LOCUS       DBNHLM010000001         2892 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1496814, whole genome shotgun sequence.
ACCESSION   DBNHLM010000001 DBNHLM010000000
VERSION     DBNHLM010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1496814"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="AC16QF_00005"
     CDS             <1..1476
                     /locus_tag="AC16QF_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6446960.1"
                     /translation="GDIVNNDGMLYLSAAERIAQGDWEAARGLYGWLFYPWLIAVISG
                     IAPLGLEASAHLVDAAMAAVMVYAFLSIVRELGGDRKVELIAAFVIVFHPYLNDSRAE
                     ILRDHGYWALYLLATFYFLKCHRNPSWPNALSWACLMALATLFRIEGSVILVALPFLL
                     LLKRDELPARRLRAFGRAYTLNALVLAAVAVWAAVEPDFTDQAGRLLDPIRKLNQLFA
                     SVGEGFQIKAQRLHDAVLTGYTDQYAVPALLAVVFLIMTDNVVRTLSPLYVVLPAFRR
                     FRARLTFPHGVPMILASLCGLHLLSALVFLVPHYYMSSRHVVAASLTLLVTIPFVLAS
                     VHDRWIQRGRGGRFQRWPYPLIAAALVFMAADGLLSVGGASKRYIREAGAWLQQNVSQ
                     DTRLYSNHRKVLFYGGRPLEFEGPPKAPLAANWISERAWRRYDYVALWERGNDGEDAQ
                     VLAEAAELELVASFANDEGDRVLIFSVGPSAAPTRAAPISP"
     gene            complement(1508..2677)
                     /locus_tag="AC16QF_00010"
     CDS             complement(1508..2677)
                     /locus_tag="AC16QF_00010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014597894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6446961.1"
                     /translation="MSIIEIISAPVKLAFCLPEYFPYGGLQNNCLRIAESLLARGHSV
                     DLYTRDWQGPVPRGCRVRVVEVRALRNHSKNVAFARKFARAMEAGRYDGVVGFMKIPS
                     LDVYYAADPCFQTRAKARRFWLYRLSGRYRALVALERSVFEPDAGTEILMLAEREIDN
                     YVRCYATPQERFHLLPPAVPRDRMAGADALEVRNALRAEFSVGPDEMLLLSVGSGFKT
                     KGVDRTIRALAALPGRLGASTSLMVVGQGDPGPFERLARRLGVGDRVRFLGGRTDVRR
                     FLFGADLLVHPAYSENTGNVLLEAMVAGLPVLTTANCGYAPRVERAGGGRVVPEPFAQ
                     ERLNAALSESLERGRLEEWSRNGIDYGRNEDLYSRPETAADIIESVIEVKAAARR"
BASE COUNT          496 a          970 c          881 g          545 t
ORIGIN      
        1 ggcgacatcg tcaacaacga cggcatgctt tacttgagcg ccgcggagcg gatcgcccag
       61 ggtgactggg aggcggcacg tgggctgtat ggctggctgt tctacccgtg gctcatcgcc
      121 gtcatcagcg ggatcgcgcc attgggcctt gaagcgtcgg cccatctcgt cgacgcggcc
      181 atggcagcag tcatggtgta tgccttcctc agcatcgtcc gggaactcgg cggcgatcgc
      241 aaagtcgagc tcatcgccgc tttcgtgatc gtcttccacc cctatctgaa cgactcgcgc
      301 gcggagattc tgcgggatca cgggtactgg gccctgtacc tgttggcaac cttctacttc
      361 ctgaaatgcc atcgcaatcc ttcctggccg aacgccctca gctgggcttg cctgatggcc
      421 ctggccacgc tcttccgcat cgagggttcc gtgatcctcg tggctttgcc gtttcttctt
      481 ctgctcaagc gcgacgagct acccgcgcgg cgcctgaggg cattcgggcg ggcgtacacc
      541 ctgaacgctc tcgtcctggc agccgtggcc gtctgggccg cggtcgagcc cgactttacc
      601 gaccaggccg gtcggctgct ggatccaatt cgcaagctga accagctgtt tgcgtccgtc
      661 ggcgagggct tccagatcaa ggcccagcgt cttcacgacg ccgtgctgac cgggtacacg
      721 gaccagtacg ctgtgcccgc tctgctcgcg gtggtgttcc tgatcatgac ggacaacgtc
      781 gttcgtacgc tgtcgccgct ttacgtcgtc ctgccggcat tccggcgctt cagggcccgc
      841 ctgacgttcc cgcacggcgt cccgatgatt ctggcttctc tgtgcgggct gcatctactg
      901 agtgccttgg tctttctggt gccccactac tacatgtcca gtcgccacgt ggtcgcggcg
      961 tcgctgacgc tgctggtgac gatccccttc gtcctcgcct cggtgcacga tcggtggatc
     1021 cagcgaggtc gtggcggaag gttccagcgc tggccctacc cgctcatcgc agcggcgctg
     1081 gtgttcatgg cggccgacgg cctgctatcc gttggcgggg cgtccaagcg gtatatccgg
     1141 gaggccggcg cctggctgca gcagaacgtt tcccaggaca cccggctgta cagcaatcat
     1201 cgaaaggtcc tgttctacgg cgggcgtccc ctggaattcg aagggccgcc gaaagcgccc
     1261 ttggcggcga actggatctc cgaacgggca tggcggcgct acgactacgt cgcgctgtgg
     1321 gagcgcggca acgatgggga ggatgcgcag gtgcttgcag aggcagcaga gctagagctc
     1381 gtcgcgagct ttgccaacga tgaaggggac cgggtgctga ttttctcggt agggccctcg
     1441 gcagccccga cccgggcggc ccctatcagt ccctgaaggt gacaagcgcg ttggtgacac
     1501 cggacgctca gcgccgcgct gcggccttga cttcgatcac cgactcgatg atgtccgcgg
     1561 cggtttccgg ccggctgtag agatcctcgt tgcgcccgta atcgataccg ttgcggctcc
     1621 actcctcgag tcttccccgc tccaggctct ctgagagcgc ggcgttgagc cgttcctggg
     1681 cgaagggttc gggaactacc cgcccgccac cggcgcgctc cacgcgaggc gcgtaaccac
     1741 agttggccgt ggtcagcact ggcagcccgg ccaccattgc ctccagaaga acgttacccg
     1801 tgttctcgct gtaagccgga tgcaccagaa gatcagctcc gaacagaaag cgccgcacgt
     1861 ccgtacgtcc acccaggaag cggacgcgat cgccaacacc cagccgccgg gcaaggcgct
     1921 cgaagggccc tggatcgccc tggccaacca ccatcagcga ggtcgacgcg cccaggcggc
     1981 cgggaagcgc ggccagcgcc cggatggtgc ggtcgacgcc cttggttttg aagcccgatc
     2041 ccacgctcag aagcagcatt tcgtctggac ccaccgagaa ctcggcacgc agcgcgttcc
     2101 gaacctccag cgcatccgcg cccgccatcc ggtcacgcgg aaccgcgggc ggcagaaggt
     2161 ggaacctctc ctgcggcgtt gcgtagcatc gtacgtagtt gtcgatctcc cgctccgcca
     2221 gcatgaggat ctcggtacct gcatcgggtt cgaataccga tcgttccagg gctaccaagg
     2281 cgcgatagcg tccgcttagc cggtacagcc agaaccgcct cgctttcgcc ctcgtctgaa
     2341 agcaaggatc cgcggcgtag tagacgtcga gactcgggat cttcatgaag cccaccacgc
     2401 cgtcgtaacg gcccgcttcc atcgcccggg cgaatttccg agcgaacgcc acattcttcg
     2461 agtggttgcg aagcgctctg acctcaacga cccgtacccg acaccctcgg ggaacgggtc
     2521 cctgccagtc acgggtatac agatccacag agtgaccccg cgccaacagg ctctccgcaa
     2581 tccgcagaca gttgttctgc agacccccat agggaaagta ctcgggaaga cagaaagcga
     2641 gtttcacggg ggcgctaatg atctcgatga tcgacatcca tgtgcacgca cgatagccgt
     2701 ttcggctctg aatcggcaat cacaggccac cctcgaagcc gcagccccgc ccatctgcta
     2761 acatcgccca acctaaatga ctggcgcacc gacgaggtag gcgaatgagg tttggctttt
     2821 tcgcaatgcc cgaacatttc ccctgggaga actggaccct cgcctacgat cgcgacatcg
     2881 accgaatggt ga
//
LOCUS       DBNHLM010000002        14215 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1616528, whole genome shotgun sequence.
ACCESSION   DBNHLM010000002 DBNHLM010000000
VERSION     DBNHLM010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 14215)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14215)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14215
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1616528"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1683)
                     /gene="leuA"
                     /locus_tag="AC16QF_00015"
     CDS             complement(37..1683)
                     /gene="leuA"
                     /locus_tag="AC16QF_00015"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017700408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR6446962.1"
                     /translation="MPFQKYRAFQAIDLPDRQWPTRTLTTAPRWCSVDLRDGNQALID
                     PMNVAEKLRMFDLLVGVGFAEIEVGFPAASQPDFDFIRRIIDEGRIPDGVAIQVLCQA
                     REELIARTVEALRGAPKVIFHLYNSTSELQRRVVFEMDRSGIIDLAVRGTRLVKAGLE
                     TLDSDITFEYSPESFTGTELDFAVDICTAVAEEWGATPDDPMIINLPSTVEMATPNIY
                     ADQIEWFVRNFSRRDSTVISLHTHNDRGTGIAATELALMAGAERVEGTLFGNGERTGN
                     CDIVTMAMNLFSQGVDPELDFRDMPRIRATVEEINKLPVHERHPYAGDLVFTAFSGSH
                     QDAIRKGMAKVDRDHWEVPYLPIDPADVGSSYKETVRVNSQSGKGGVGFLLEEHFGIA
                     LPREMLVEFSAVVQQLTESQGREIKTDEILEALKKEYVVDSGPYRLVDFDLLTGRTSD
                     QRCIARVEVNEQQVTIDGEGSGPIEAFVNGMVETLNEPLNIIDYHEHAMTTGKDADAF
                     CLVAIDDPKQGRCYGVGVSRNTITASFMAIVSALNRRWKA"
     gene            complement(1978..3165)
                     /gene="purT"
                     /locus_tag="AC16QF_00020"
     CDS             complement(1978..3165)
                     /gene="purT"
                     /locus_tag="AC16QF_00020"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010220253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HIR6446963.1"
                     /translation="MTSIGTPLAGNATRVMMLGAGELGKEVVIELQRLGVEVIAVDRY
                     ANAPGMQVAHRSHVISMLDGDALREVIALEKPDMVVPEIEAIATEVLVELESEGLTVI
                     PTARAARLTMDREGIRRLASETLGVRTSPYRFAATREEYDEAIQEIGVPCVVKPVMSS
                     SGKGQSTVRTAADAAAAWDYAQAGARGGADKVIVEGFVDFDYEITLLTVRHVGGTSFC
                     APIGHRQAGGDYQESWQPQPMAEAALAECKRIGELVTGELGGRGLFGMEFFIAGDQVY
                     FSEVSPRPHDTGMVTLLSQDLSEFALHARAILGLPIPHIQQHGPSASSVVLVEGESRA
                     VRFEGFEAALAEPGTDLRLFGKPEVAGERRMGVALARGRDVDDARKKANAAAAAIRWS
                     FDD"
     gene            complement(3162..3701)
                     /locus_tag="AC16QF_00025"
     CDS             complement(3162..3701)
                     /locus_tag="AC16QF_00025"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003178404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR6446964.1"
                     /translation="MLYQLGERRVCAEGAHFIAPGAMLTGSVTLKANVNVWFNAVIRG
                     DVEPILVGANSNIQDCAVLHTDAGCPLTIGDYVTVGHKAVLHGCDVGDNTLIGINAVV
                     LSGARIGRNCIIGANALVTEGKVIPDNSMVLGSPGKVVRELVDEQIAGLRVSAEGYVE
                     RARWYLAELSEQESVDITS"
     gene            3876..4214
                     /locus_tag="AC16QF_00030"
     CDS             3876..4214
                     /locus_tag="AC16QF_00030"
                     /inference="COORDINATES: protein motif:HMM:NF022777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3135 domain-containing protein"
                     /protein_id="HIR6446965.1"
                     /translation="MKSRLPGFDTLVDMARNDPDRLESLRRSLTDAVIAEASCDDTRR
                     RLQGLQFRVDMERRRAATPLAATIRISEMMARSLADLHRSLVAPETLEEDLTRKPPRR
                     SDNVIEFPPS"
     gene            4266..4817
                     /locus_tag="AC16QF_00035"
     CDS             4266..4817
                     /locus_tag="AC16QF_00035"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003293638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6446966.1"
                     /translation="MKYCSACGFELVLRIPEGDNRERFLCEQCDTIHYQNPRIITGCL
                     AIWEEKVLLCRRSINPRRGFWTLPAGFLENGETAQAGAARETWEEARARVVIDDLYTM
                     FSLPHISQVYLFYKARLLDGSYAAGAETAEAGLFDEEEIPWDDLAFPVVRDTLQHYFQ
                     DRIHDHFRVHTGDIIVQRDRRSD"
     gene            complement(4828..8658)
                     /gene="purL"
                     /locus_tag="AC16QF_00040"
                     /gene_synonym="purI"
     CDS             complement(4828..8658)
                     /gene="purL"
                     /locus_tag="AC16QF_00040"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HIR6446967.1"
                     /translation="MLTLEGPPARSAFRLNKLSGQLGLPEGALYAQYVHLLAVHQPLA
                     GQALERAQALLRYGPETGLPAPTGDLVATVVPRAGTISPWSSKATDIFAICGLDQVTR
                     VERGVRWFADRGLSDVQLALLYDRMTERAVGEADFPSVFANLPPKPMSTVALQAGGLA
                     ALEEANRALGLALSADEIEYLASAYLELGRDPNDVELMMFAQANSEHCRHKIFNASWS
                     IAGEPQPLSLFEMIRNTYRSINGAGILSAYSDNAAVIEGHRIERFGVDSASHEYGYDE
                     AAVHVLMKVETHNHPTAIAPYPGAATGSGGEIRDEGAVGRGSKPKAGLTGFTTSHLNI
                     PGYGQPWELETGKPEHMVSALDIMVEGPVGAAAFNNEFGRPALAGYFRTFEQTVAGDP
                     RQVRGYHKPVMIAGGVGSIRPEHVQPTAFPAATALIVLGGPAMLIGLGGGAASSMGSG
                     ESSSDLDFASVQRDNAEIERRCQEVIDRCCALGERNPILLIHDVGAGGLSNALPELVK
                     DAGVGGHFQLRDVPNADPGMTPLEIWCNEAQERYVLAIAEEHLPGFAEICHRERCPFA
                     VVGRAVADPHLTVDDAEFANSPVDLPLSVLFGKPPKMQRSFAPRALTPPGFSHNGLVL
                     AEAIDRVLRFPCVASKQFLITIGDRSITGLVAREQMVGPWQVPCADVAVTLSGYRSYA
                     GEAFAMGERSPTALIDPAASARMAVGEALTNLCAAPVGDLGRVVLSANWMAAAGADAE
                     EQALFEGVRAVGMELCPALGIAIPVGKDSLSMRTRWDDREVTSPLTLIVSAFAPVTDA
                     RRTLTPELKTTPDTRLLLVDLGQGRNRLGGSVLAQTYGQLGAEAPDVNSPDMLGAFFR
                     AIQELNEQGMLLAYHDRSDGGLIACVLEMAFAGRCGLDLRVDGEDVFGALFAEELGAV
                     VQVAEKDLEAVRAAFGEIPCAVIGCPRDDGVVVINGARAELYRSDRAALQEAWAETSY
                     QIQRLRDNPDCADEERSGIRAADPGFSSRVTFDVDEDVCAPYVGSSRPRIAVLREQGV
                     NGQIEMAAAFERAGFLPVDVHMSDVLEGRTRLLDFPALAACGGFSYGDVLGGGGGWAK
                     SILFDDRARDQFVEFFARDRLALGVCNGCQMMSALKEIVPDAGSWPRFVRNRSEQFEG
                     RTVLVRVNEVDSPWLEGMAGSVLPIAVAHGEGRAEFADAGTELDFWESRRDQVCLQYV
                     DNHHRPTELYPANPNGAGRGLAGLTGAFGRVLIMMPHPERVFRAYQNAWRDQSWQEDG
                     PWMRLFRNARVALR"
     gene            complement(8686..9132)
                     /gene="tadA"
                     /locus_tag="AC16QF_00045"
     CDS             complement(8686..9132)
                     /gene="tadA"
                     /locus_tag="AC16QF_00045"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017131735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIR6446968.1"
                     /translation="MSHVDWMRLALEEAGRAAASGEVPVGAVVVRDGELLGSGFNCPI
                     SSSDPTAHAEIVALRAAAKRSGNYRLAGAAMYVTVEPCTMCAGALVHARIATLVYGAL
                     EPRSGAVTSTATVLENPLLNHRVEVVGGVLARECAEVLRAFFAERR"
     gene            complement(9157..10734)
                     /gene="guaA"
                     /locus_tag="AC16QF_00050"
     CDS             complement(9157..10734)
                     /gene="guaA"
                     /locus_tag="AC16QF_00050"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR6446969.1"
                     /translation="MADKYDIHDQRLLIIDFGAQYTQLIARRVREAGVYCEIHPFDVP
                     EDFIREFAPRGVILSGGPESVTFADTPRIPSIVFELGVPILGICYGMQAMAEQLGGSV
                     EASDVHEFGHAEIEIVETAELLGTVRDTAGGHLPVWMSHGDRVVRTPPGFEVTASTPS
                     APVAAMEHREKRMYGVQFHPEVTHTQRGEEMLAHFARTLCRCDGLWTAANIVEDAIAH
                     ARDLVGDERVVLGLSGGVDSSVVAALLSRAIGDQLTCVFVDNGLLRKGEREEVERAFA
                     PTNALALNLVSVPAAEEFLGRLAGVVDPEAKRKVIGNTFIEVFDREAHKISGVSWLAQ
                     GTIYPDVIESAAGKYGKAHVIKSHHNVGGLPERMHLKLVEPIRELFKDEVRKIGMHLG
                     LPRELVYRHPFPGPGLGVRVLGEVTAARADVLREADAIYLEELRAAGMYDAVSQAFAV
                     LLPVKSVGVVGDARRYADVIALRAVETTDFMTARCARLPYELIERVSNRIINEIPHVS
                     RVVYDVSSKPPATIEWE"
     gene            complement(10735..12204)
                     /gene="guaB"
                     /locus_tag="AC16QF_00055"
     CDS             complement(10735..12204)
                     /gene="guaB"
                     /locus_tag="AC16QF_00055"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR6446970.1"
                     /translation="MLRIAHDALTFDDVLLLPAYSQCLPSEVSLATKLTRDIHLNIPL
                     MSAAMDTVTEARLAIAIAQEGGVGIVHKNMDIESQAREVRAVKKHESGIIRDPITIHP
                     DKTVADLIELTMEHKISGVPVVDGRRLVGIVTGRDVRFERRMDAKVRDVMTTKEHLVT
                     ADEEASFEKITDLLHKHRIEKVLLINDRFELKGMVTVKDITKAQAYPNACKDHRGQLR
                     VGASVGTSPDTDDRVAALVEAGVDVLVVDTAHGHSQRVLDRISWIKQRYPDVNVIGGN
                     VATADGAKALLSAGVDAVKVGIGPGSICTTRMVTGIGVPQITAIAEAVAVADSEGVPV
                     IADGGVRYSGDIAKAIAAGASVVMVGGLLAGTDEAPGEVELYQGRTYKSYRGMGSLGA
                     MTESNGSSDRYFQEAEGDHRKLVPEGIEGRVPYRGPVGPIVHQLMGGLRASMGYTGSN
                     DIETMRTQPRFVRVSAASMVESHVHDVAITKEAPNYPVS"
     gene            12263..13654
                     /gene="xseA"
                     /locus_tag="AC16QF_00060"
     CDS             12263..13654
                     /gene="xseA"
                     /locus_tag="AC16QF_00060"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR6446971.1"
                     /translation="MLASQADRPVYSVSELAHEARILIEERFDLVWVEGEISNFRRPA
                     SGHWYFTLKDRSAQVRCAMFAGRNRQVRFAPEDGMQVLIRGRVSLYEPRGDFQVIVEH
                     AEPAGEGALRQAFEALKTKLAEEGLFAIERKRPLPALPRHLAIVSSASGAALQDVLHV
                     IERRFPALTVTLLPVSVQGNEAEAQIRRALARVDQVGADVVLITRGGGSLEDLWAFNL
                     ESVARAVAACPIPTVVAVGHQTDFVIAEFVADLRAPTPSAAAELITPDAAELAGRVGL
                     LQQRLVRGLSADVRLRQALVGRLRAQLVDPRQMVTRSMQRADDLEERLRLALGNRLER
                     NRNRLWALQRSLRAFRPEPLLRQQAARVTATRHALGKSLQRALGGSAERLGSAARTLD
                     AVSPLHTLARGYAVLAEPAADGLGRPVTAIAQVAPGATIDAHLHDGRLRVEVVSVNEE
                     SALQAAQTHVEEH"
     gene            13655..>14215
                     /locus_tag="AC16QF_00065"
     CDS             13655..>14215
                     /locus_tag="AC16QF_00065"
                     /inference="COORDINATES: protein motif:HMM:NF037811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HIR6446972.1"
                     /translation="MLARAALATLCWLTPMLLRADAVPGGLYAYGPVPPASEVRFDDR
                     RVLVIDETALVGIPLSASPGTHSLTITYASGESVSHQFEVVAKQYPEQRLTITDRKMV
                     NPDPEDLERIRTESARMREQYLSFTEAMQRLRPFHKPVNGVTSSPFGRRRILNDQPRN
                     PHSGLDIAADHGTPVSAPAPARVSLTG"
BASE COUNT         2531 a         4875 c         4371 g         2438 t
ORIGIN      
        1 gcccgccagg gccctcgcag ccaagccagc ccgtcttcag gccttccagc gccggttcag
       61 cgccgagacg atcgccatga acgacgcggt aatcgtattc cggctcacgc cgacgccgta
      121 gcagcgcccc tgcttgggat catcgatggc gaccagacag aacgcatcgg cgtccttccc
      181 cgtcgtcatg gcgtgctcgt gatagtcaat gatgttcaaa ggctcgttga gcgtctcgac
      241 catgccgttg acgaacgcct cgatggggcc ggacccttca ccgtcgatgg tcacctgctg
      301 ctcgttgacc tctacccgcg caatacagcg ctgatcagac gtccggccgg tgagcaggtc
      361 gaagtcgacc agccgatagg gaccggaatc gaccacatac tctttcttca gggcctcgag
      421 gatctcgtcg gtcttgatct cccggccctg actctcggtc agctgctgca ccacggcgct
      481 gaactcgacg agcatttccc gcggtaaggc tatgccgaag tgctcctcga gcagaaaacc
      541 caccccgccc ttgccggact ggctgttcac ccggaccgtt tccttgtagg aggaaccgac
      601 gtcggcggga tcgatgggca ggtagggcac ttcccagtga tccctgtcca ccttggccat
      661 gcctttgcgg atcgcatcct ggtgggagcc ggagaaggcg gtgaacacca gatctcccgc
      721 atagggatgg cgctcgtgaa ccggcagctt gttgatctcc tccaccgtcg cccggatacg
      781 cggcatgtca cggaagtcga gctcggggtc cacgccctgg ctgaagagat tcatcgccat
      841 ggtgacgatg tcgcagtttc ccgtgcgctc gccgttaccg aacagcgtcc cttcaacccg
      901 ctcggcgccc gccatcagcg caagctccgt agcggcgatc ccggtgccgc gatcgttgtg
      961 ggtatgaagg ctgatcaccg tgctgtcccg cctcgagaaa ttccgtacga accactcgat
     1021 ctgatcggcg tagatgttgg gtgtggccat ctccaccgtc gacggcaggt tgatgatcat
     1081 cggatcgtcc ggggtggcgc cccactcctc ggccacggcg gtgcagatgt ccacggcaaa
     1141 gtcgagctcc gtaccggtaa agctttccgg cgagtactcg aacgtgatat cggaatccag
     1201 ggtctcgagg cccgccttga ccaggcgcgt tccccgcacc gccaggtcga tgatcccgga
     1261 gcggtccatc tcgaacacca cgcggcgctg caattccgaa gtcgagttgt ataggtgaaa
     1321 gatcaccttc ggcgcgccac gaagcgcctc cacggttctg gcaatcagct cctcgcgagc
     1381 ctggcagagc acctgaatgg cgacgccgtc cgggatcctg ccctcgtcga tgatgcggcg
     1441 aatgaaatcg aaatcgggct gcgaggcggc cggaaaaccc acttcgatct cagcaaaccc
     1501 gacaccgacc agcaggtcga acattcgcag tttctcggcc acgttcatcg ggtcgatcag
     1561 cgcctgattg ccgtcgcgca gatccacgct gcaccaccgc ggagccgtgg tcagggtccg
     1621 ggtcggccat tgccggtccg gcaggtcgat ggcctgaaag gcacggtact tttggaaagg
     1681 catggtgttc gccgcgcgac cgccggttgg ctggcgctgg gcccggaagg gggattgggt
     1741 ttgctcactc attgttcttc gcctgcgagg tctttctcgc tgctgctggt cccggaggat
     1801 cgttaaggtt tcgtatgggg tgggcggaat gcccttttgc gtaagtattg gccggctacg
     1861 ccggcagtcg cagcagcgcc agaagagaca gatttggacg gtgatgagag ttcaattcag
     1921 ctcccgaatc gtggccgcga ctatagccag ccggggagga tttgccaacc ctgctgatca
     1981 gtcgtcaaac gaccaccgaa tggctgcggc tgccgcgttg gccttcttgc gggcatcgtc
     2041 cacgtcccgc ccccgggcca gcgcgacgcc catgcgccgc tcgcccgcta cctcgggctt
     2101 tccgaacaga cgcaaatccg tgcccggctc cgcgagcgcg gcttcgaacc cctcgaacct
     2161 gacggcgcgc gattctccct ccaccagcac gacgctggat gccgagggcc cgtgctgctg
     2221 gatgtgagga atcggcaatc cgagaatggc ccgggcgtgc agcgcaaact ccgagagatc
     2281 ctggcttagg agcgtcacca ttccggtgtc gtgggggcgc gggctcacct cgctgaaata
     2341 gacctggtcg cctgcgatga aaaactccat accgaagaga ccacgacctc cgagctcacc
     2401 ggtgaccagc tcgcctatgc gcttgcattc cgccaacgcc gcttcggcca tgggctgagg
     2461 ctgccaggac tcctgatagt cgccgccggc ctgacgatgc ccgatgggcg cacagaacga
     2521 cgtgcctccg acatggcgga cggtgaggag cgtgatctcg taatcgaaat ccacgaaacc
     2581 ctccacgatc accttgtcgg caccacccct ggccccggcc tgcgcgtagt cccaggcagc
     2641 ggcagcgtcg gccgccgtgc gaaccgtgga ctggcccttg cccgaggagc tcatcacggg
     2701 cttgaccaca cagggaacgc cgatctcctg tatggcctcg tcgtactctt cgcgcgtcgc
     2761 cgcaaatcgg tagggcgagg tgcgcacgcc cagggtctcg gaagccaggc gccggatgcc
     2821 ctcgcggtcc atcgtgagcc gggctgccct ggccgtcggg atgaccgtca ggccctcgga
     2881 ttccaactcc accaggacct cggtggcgat ggcttcgatc tcggggacca ccatgtcggg
     2941 cttctccagc gcgatcacct cacgaagcgc gtcgccgtcg agcatgctga tcacgtggct
     3001 gcgatgggcg acctgcattc ccggcgcgtt ggcgtatcgg tcgacggcaa tgacctccac
     3061 gccgagcctc tgcagctcga tgaccacttc cttaccgagc tcgccggcac cgagcatcat
     3121 tactcgcgtt gcgttgcccg ccagcggcgt ccctatgctc gtcatgacgt gatgtccaca
     3181 ctttcctgtt cggataactc ggcaagatac caccgggcgc gctcgacata tccttcagcg
     3241 ctcactcgca gccccgcaat ctgctcatca accagctccc tgaccacctt gccgggcgac
     3301 cccagcacca tgctgttgtc aggaatgacc ttgccctcgg ttaccagcgc attcgccccg
     3361 atgatgcagt tgcgtccgat ccgggccccg ctcagcacga cggcgttgat gccgatgagg
     3421 gtgttgtcgc caacgtcgca accgtgcaga acggccttgt gccccacggt gacgtagtcc
     3481 ccgatcgtca gcgggcagcc cgcatcggtg tgcaaaaccg cgcagtcctg gatgttgctg
     3541 ttggcaccga ccaagatcgg ttccacgtca ccgcgaatga ccgcgttgaa ccagacgttc
     3601 acgttggctt tcaaggtcac cgatcctgtg agcatggccc ctggtgcaat gaaatgcgca
     3661 ccctcggcgc atacacggcg ttcaccgagc tgataaagca ctgctgcgtc acccaacccc
     3721 aaagcggcag agtgcagagt aatgcaccaa ccgccggttc acaaagccct tacacaaatt
     3781 acgtggcgat aacgcccacc cacaggcata gttttctgcg gggaacctcc agcacgacct
     3841 tgagccacaa cgataactag cgagtagggt cgcttatgaa atcccggctg ccgggcttcg
     3901 atacgctggt ggatatggct cgcaacgatc cagaccgact ggaatcgttg cgacgctcac
     3961 tgactgacgc cgtcattgct gaagccagct gcgacgacac cagacgccga ctacagggtc
     4021 tgcagttccg tgtggacatg gaacggcgcc gtgcggcaac acctctcgcc gcgaccatcc
     4081 gcatctcgga gatgatggct cgctcgctcg ccgacctgca cagatcgctg gtggcgccgg
     4141 aaacgctcga ggaagatctc accagaaaac cgccccgacg gagcgacaac gtcatcgagt
     4201 ttcctccgag ctgagttgcc cgaagccgcc tgcgacgggc cgcgcagtcg cgttatactc
     4261 gatgcatgaa gtactgcagc gcctgcgggt tcgagttggt cttacgcatt cccgagggag
     4321 acaaccggga aaggttcctg tgcgagcagt gcgacaccat tcactaccag aacccacgga
     4381 tcattacggg ctgccttgcc atctgggaag agaaggtcct gctgtgcaga cgctcgatca
     4441 acccgcgccg gggtttctgg acgctgccgg ccggttttct ggagaacggc gagaccgccc
     4501 aggccggggc cgcgcgtgaa acctgggagg aagcgcgggc ccgggtcgta atcgacgacc
     4561 tctacacgat gttcagcctc ccgcacatat cccaggtcta cctcttctac aaggcgcggc
     4621 ttctcgatgg atcctacgct gccggggcgg agaccgccga ggcgggcttg ttcgacgagg
     4681 aggaaatacc ctgggacgac ctggcgttcc cggtggtgcg cgacaccctt cagcattact
     4741 tccaggaccg catccacgat cactttcggg ttcacaccgg cgacatcatc gttcagcggg
     4801 atcggcgctc ggactgaacc gagcgcccta gcgcagcgcc acccgggcgt tgcgaaacaa
     4861 gcgcatccac ggcccgtctt cctgccacga ctgatcccgc caggcattct gataggcgcg
     4921 aaatacccgc tcgggatgcg gcatcatgat caataccctg ccaaaggcgc cggtcagccc
     4981 cgccaggccg cgaccggcac cattgggatt ggccggatac agctcggtcg gccgatgatg
     5041 gttgtccaca tactgcagac agacctgatc ccgccggctc tcccagaaat ccagctccgt
     5101 cccggcatcg gcaaactcgg cccggccttc gccatgggcg accgcgatgg gcagcacgct
     5161 tccggccatg ccctcgagcc atggagagtc cacctcgttg acgcgaacca gaaccgtgcg
     5221 accttcgaac tgctctgagc ggttgcggac gaagcgaggc cagcttcccg cgtccggaac
     5281 gatctccttc agggcagaca tcatctggca accgttgcag acacccagcg ccaagcggtc
     5341 gcgcgcaaag aactcgacga actggtcgcg agcccgatcg tcgaacagga tgctcttcgc
     5401 ccaaccgccc ccgccgccga gcacgtcgcc ataggaaaac cctccgcatg ccgcgagagc
     5461 cggaaagtcg agaagccggg tcctcccttc cagaacgtcg ctcatgtgca catccacagg
     5521 caggaaaccg gcccgctcga aagccgccgc catctcgatc tgcccgttca cgccctgctc
     5581 gcgcagcacg gcgatgcgcg ggcgcgagct gcccacatag ggagcgcaga cgtcctcgtc
     5641 gacgtcgaag gtcacgcggc tggagaatcc aggatccgct gcccgaatgc cgctgcgttc
     5701 ctcgtcggcg caatcgggat tgtcgcgcag gcgctgtatc tggtagctgg tttccgccca
     5761 ggcttcctgg agcgccgcgc gatccgatcg gtacagctcc gcccgggcgc cgttgatgac
     5821 gaccaccccg tcatcgcgag ggcatccgat gaccgcgcac ggtatctcgc cgaatgccgc
     5881 gcgaaccgct tccagatctt tttccgccac ctgaaccacc gccccgagct cttcggcaaa
     5941 caaggcgcca aacacgtctt cgccatcgac gcgcagatcc agcccgcagc gcccggcgaa
     6001 ggccatttcc agcacgcagg caatgagacc gccatccgaa cggtcgtgat aggccagcag
     6061 catgccctgc tcgttcagtt cttggatcgc acggaaaaag gccccaagca tatcgggcga
     6121 gttcacatcg ggagcttccg ccccgagctg accataggtc tgggcaagca cgctgccgcc
     6181 cagccggttt ctgccctgac cgagatccac cagcaggagg cgggtatcgg gggtggtctt
     6241 gagctcgggc gtgagggtac ggcgcgcatc cgtcaccggc gcaaacgcag agacgatgag
     6301 cgtcagcggc gacgtcactt ccctgtcgtc ccaacgagtg cgcatggaaa ggctgtcctt
     6361 gcccaccggt atggcaattc cgagggccgg acagagttcc atccccaccg cccgtacgcc
     6421 ttcgaacagc gcctgctctt ctgcatcggc ccccgcggcg gccatccagt ttgccgacag
     6481 caccaccctg ccaagatcgc cgacaggcgc cgcacacagg ttggtcaagg cttcgcccac
     6541 ggccattcga gcggatgcgg cgggatcgat gagcgcagtc ggtgaccgct cgcccatggc
     6601 aaacgcctcg ccggcatagc tccggtatcc ggatagcgtg accgcaacat ccgcgcacgg
     6661 cacctgccac gggcccacca tctgctcccg cgccaccagc ccggtgatgc tccgatcgcc
     6721 gatggtgatc agaaactgct tgcttgccac acacggaaag cgcaaaaccc ggtcgatggc
     6781 ctctgcgaga acgagaccat tgtggctgaa cccaggcggg gtgagcgccc gcggtgcgaa
     6841 cgaacgctgc atcttaggcg gcttgccgaa cagcacggaa agcggcaggt ccacgggcga
     6901 gttggcgaac tcggcatcat cgaccgtcag gtgcgggtcg gccacggccc ggcccacgac
     6961 ggcaaagggg caacgctcgc gatggcagat ctcggcgaac cccggcagat gctcttcggc
     7021 aatcgccagc acgtagcgtt cctgtgcctc gttgcaccag atttccagag gcgtcatgcc
     7081 cgggtccgcg ttgggcacgt cacgcagctg aaaatgcccg ccgacgcccg cgtccttgac
     7141 cagctccgga agggcattgg acaacccgcc ggcgccgaca tcgtgaatga ggagaatggg
     7201 atttcgctct cctaaagcgc agcagcgatc gatcacctcc tggcagcggc gctcgatctc
     7261 cgcgttgtcc cgctgaaccg aggcaaaatc gaggtcggac gagctctctc ccgatcccat
     7321 gctcgaggcg gcgcccccgc cgagcccgat gagcatggcc gggccaccga gcacgatcag
     7381 cgcggtcgcc gccgggaaag cggtgggttg gacgtgctcc gggcggatgc tgccgacgcc
     7441 gccggcgatc atgacgggct tgtgatagcc gcgaacctgg cgggggtcgc cggcaacggt
     7501 ctgctcgaaa gtacggaaat agccggccag agccggtctt ccgaactcgt tgttgaaggc
     7561 ggccgcaccc acggggcctt ccaccatgat gtccagcgcg ctcaccatgt gttccggttt
     7621 gccggtctcg agctcccagg gctggccata gccgggaatg ttgaggtgcg aggtcgtgaa
     7681 gcccgtaagg cccgccttcg gcttcgaccc tcggcccacg gcgccctcat cgcggatctc
     7741 gccgccggac ccggtggcgg cccccgggta cggggcgatg gccgtcgggt gattgtgggt
     7801 ctctaccttc atcaacacgt ggacggctgc ttcgtcgtag ccgtactcgt gagacgcgga
     7861 gtccacaccg aaacgctcga tgcgatgccc ctcgatgacg gctgcgttgt cggaataggc
     7921 gctgagaatc ccggccccgt tgatcgaccg gtaggtattc ctgatcatct cgaacagcga
     7981 aagcggctga ggttcgccgg cgatgctcca cgacgcgttg aatatcttgt ggcggcagtg
     8041 ctccgagttc gcctgagcga acatcatcaa ttcgacgtcg ttcgggtcac gcccgagctc
     8101 cagataggcg ctcgcgaggt attcgatctc atccgcggac agcgcaaggc ccaacgcccg
     8161 gttggcctct tccagcgccg ccaggccgcc cgcctgcaga gcaaccgtgc tcatgggctt
     8221 tggcggcagg ttcgcgaaaa ccgaggggaa gtccgcctcg ccaaccgccc gctcggtcat
     8281 gcggtcgtaa agcagcgcaa gctggacatc gctcaggccc ctgtccgcaa accaccgcac
     8341 gccccgttcg acccgggtca cctggtccag accgcagatg gcgaagatgt cggtcgcctt
     8401 gctggaccac ggcgagatcg tgccggcccg aggaaccacg gttgcgacca gatcacccgt
     8461 cggcgccggc agccccgttt ccggaccgta gcgaagcagc gcctgcgcgc gctccagcgc
     8521 ctgtcccgca agcggctgat ggacggccag gagatggacg tactgagcgt acagcgcacc
     8581 ttccggcagg cccagctgcc cgctcagctt gttcaggcga aaggcggagc gggccggcgg
     8641 cccttcgagc gtcaaaatct ccatgaaccg atcctcatcc gatgctcacc gccgctcggc
     8701 aaagaacgcg cgcaaaacct ccgcgcactc gcgcgcaagc acgccgccca ccacctcgac
     8761 ccggtgattc agcaacgggt tttccagaac ggtcgccgtg ctggtcaccg caccggatcg
     8821 cggctccaac gcgccgtaga ccagggtcgc gatccgcgcg tggacgaggg ctccggcaca
     8881 catggtgcac ggttccaccg tcacgtacat cgcagcaccc gcaagccggt agttgccgga
     8941 ccgtttagcg gcagcacgca gcgcaacgat ctccgcatgt gccgtggggt cgctgctgct
     9001 gatgggacag ttgaagccac tccccagcag ctccccgtcc cgaaccacca ccgcgcccac
     9061 gggcacctct cccgaggcgg ctgcgcgacc cgcctcttct agagccaggc gcatccagtc
     9121 aacgtggctc atcgccctcc cgtgccaacc acacggtcac tcccactcga tggtcgctgg
     9181 cggtttgctg gagacgtcgt aaaccacccg cgaaacgtgg gggatctcat tgatgatccg
     9241 gttggataca cgctcgatca gctcgtaggg caaccgtgca cagcgggcgg tcatgaagtc
     9301 cgtggtctcc accgccctca gggcgatgac gtccgcgtaa cgccgtgcgt cgcctaccac
     9361 gcccaccgac ttcaccggca gcagcacggc aaacgcctgg ctgacggcgt cgtacattcc
     9421 cgcagctcgg agctcttcga ggtagatggc gtccgcttcg cgcaggacgt cggcacgggc
     9481 ggcggtcacc tcgccgagca cccgcacgcc caggcccgga ccgggaaacg ggtgccggta
     9541 gaccagctcc cgaggcagcc ccagatgcat cccgatcttg cggacctcgt ccttgaacag
     9601 ctcgcgaatc ggctccacca gcttcagatg catccgttcg gggaggccgc cgacgttgtg
     9661 atgggacttg atgacatgag ccttgccgta cttgcccgct gccgactcga tgacgtcggg
     9721 ataaatggtg ccctgagcca gccagctcac cccggagatc ttgtgcgctt cacgatcgaa
     9781 gacctcgatg aacgtattac cgatgacctt gcgtttcgcc tcggggtcca cgacaccggc
     9841 caggcgcccc agaaactctt ccgcggccgg cacgctgacc aggttgagcg ccagagcgtt
     9901 ggtcggcgca aaagcacgct ccacctcttc ccgctcgccc ttgcgaagca ggccgttgtc
     9961 cacgaacacg cacgtcagct gatccccgat ggcccgggag agcagagcag cgaccacgct
    10021 ggaatccacg cctccggaca accccagcac gaccctttcg tctccaacca ggtcgcgggc
    10081 gtgggcgatg gcgtcctcga cgatgttggc ggccgtccac aggccatcgc agcgacacag
    10141 cgtccgcgcg aagtgggcaa gcatctcttc gccccgctgg gtgtgcgtca cctcaggatg
    10201 aaactgcacg ccgtacatgc gcttttcgcg atgctccatc gcagccacgg gcgccgacgg
    10261 ggtgctggcg gtgacctcga agcccggagg cgtcctcacc acccggtcgc cgtggctcat
    10321 ccagaccggc agatgcccgc cggcggtgtc acgcaccgtg ccgagcagct cggccgtctc
    10381 gacaatctcg atttctgcgt ggccgaactc atggacgtcg gacgcttcca cggaaccgcc
    10441 cagctgctct gccatggcct gcatgccgta acagatgccg aggatgggca cccccagctc
    10501 gaacacgatg gacgggatgc gcggcgtgtc cgcaaacgtt accgactccg ggccgccaga
    10561 gaggatcacc ccccgcggcg cgaactcgcg tatgaaatcc tccgggacgt cgaaggggtg
    10621 aatctcgcaa taaacgccgg cctcgcgcac ccggcgggca atcagctggg tgtactgggc
    10681 accgaaatcg atgatcagga ggcgctgatc atgtatgtcg tatttgtccg ccattcagct
    10741 gacgggatag ttcggcgctt ctttggtgat cgccacgtcg tgaacgtgac tctccaccat
    10801 gctggccgcg ctcactcgca cgaagcgagg ctgggttcgc atggtctcga tatcgttcga
    10861 gccggtatag cccatcgacg ccctgagccc gcccatgagc tgatgaacga tggggcccac
    10921 aggcccacgg taaggcacgc gcccttcgat cccctcggga acgagcttgc gatgatcccc
    10981 ctctgcttcc tggaagtacc gatcgctgga cccgttggac tcggtcatcg cacccagcga
    11041 gcccatgccg cgataggact tgtaggttct gccctgatag agctctacct ctccgggcgc
    11101 ttcatcggtg ccggcgagca gcccgccgac catcaccacg ctggccccgg ccgcgatggc
    11161 tttggcaatg tcgcccgagt aacggacacc tccatcggca atgacgggca ccccttccga
    11221 gtcggcaacc gccaccgctt cggcgatggc cgtgatctgc ggaacaccaa tgccggtaac
    11281 cattcgcgtg gtgcagatgg agccggggcc gatgccgacc ttgaccgcat ccacgccggc
    11341 ggaaagaagc gctttcgcgc cgtccgcggt agccacgttg ccgccgatga cgttgacgtc
    11401 cggatagcgc tgcttgatcc agctgatgcg gtccagcacc cgctgggagt gcccgtgagc
    11461 cgtatccacc accagcacgt cgacgccggc ttccacgagg gccgcgaccc ggtcgtcggt
    11521 gtcggggctg gtacccacgc tcgcaccgac ccggagctgt ccccggtggt ctttgcaggc
    11581 attgggataa gcctgggcct tggtgatgtc cttgacggtc accatgcctt tgagctcgaa
    11641 gcgatcgttg atgaggagga ctttctcgat gcgatgcttg tgaaggagat cggtgatctt
    11701 ctcgaagctt gcctcctcgt ccgcggtgac gaggtgctcc tttgtggtca tcacgtcgcg
    11761 gaccttggcg tccatgcgac gctcgaaccg cacgtcgcgt cccgtgacga tgccgaccag
    11821 ccggcgtcca tccaccaccg gcacgccgga gatcttgtgt tccatggtca gctcgatcag
    11881 atccgcgacc gttttgtccg gatggatggt gatcgggtca cggatgatgc cactttcgtg
    11941 cttcttgacc gcccggactt cacgggcctg gctttcgatg tccatgttct tgtgcacgat
    12001 gccgaccccg ccctcctgcg cgatcgcaat agcaagccga gcctccgtca ccgtgtccat
    12061 ggcagcggac atgagcggga tattgaggtg gatgtctctg gtcagcttcg tcgcgagcga
    12121 aacttctgaa ggcaagcact gggagtaggc gggaaggagc agaacgtcgt cgaacgtgag
    12181 cgcgtcatgc gcaatacgca acattggtca ctccgcaaaa gcggcattct atcggcgcac
    12241 cccgatcatg taaactcgac ccatgctcgc cagccaagca gatcgccccg tctacagcgt
    12301 cagcgaactc gcccacgagg cgagaatcct catcgaggag cgcttcgacc tggtctgggt
    12361 ggagggcgag atctccaact tccggcgtcc cgcatccggc cactggtact ttacgctcaa
    12421 ggatcgctcc gcccaggttc gctgcgcgat gttcgccggc cgcaaccgcc aggtacgctt
    12481 cgctcccgag gacggcatgc aggtcctgat caggggccgt gtcagccttt acgagccgcg
    12541 cggcgacttc caggtcatcg tcgagcacgc ggagcccgcc ggcgaaggcg cgctgcgcca
    12601 ggccttcgag gccctgaaga ccaagctcgc agaggaaggt ctcttcgcga ttgagcgtaa
    12661 gcggccgctg cccgcgctgc cccgccacct ggcaatcgtc tcgtcggcct ccggcgcggc
    12721 tctccaggac gtcctccacg tcatcgagcg gcgctttccg gcgctgaccg tcacgctgct
    12781 gccggtcagc gtgcagggga acgaggccga ggcccagatc cggcgcgcgc tggcgcgggt
    12841 cgatcaggtc ggcgccgacg tggtgctgat cacgcgcggc ggagggtctt tggaggatct
    12901 ctgggcgttc aacctcgaaa gcgtcgcccg cgcggtggca gcgtgcccga tacccacggt
    12961 ggtggccgtg ggccaccaga ccgacttcgt catcgccgag tttgtggccg acctgcgcgc
    13021 acccacgcca tccgccgccg ccgagctcat cacgccggat gccgcagagc tggccgggcg
    13081 cgttgggctg cttcaacagc gactggtccg cgggctttcc gccgacgtgc gcttgcggca
    13141 ggcgctggtg gggcggctgc gagcccagct cgtcgatccg cgccagatgg tgacccgcag
    13201 catgcagcgt gcggacgatc tggaggagcg cctgcggctg gccctcggca accggctgga
    13261 gcggaaccga aaccggctgt gggcattgca gcgcagcctc cgggccttcc gtccggagcc
    13321 cctgttgcgc cagcaagcgg ccagggtcac cgccacgcgt cacgccctgg ggaaatccct
    13381 tcaacgcgcc ctggggggct ccgcggagcg cctcggatcg gccgcgcgca cgctggatgc
    13441 cgtcagtcct ctgcacacgc tggcgcgtgg ctatgccgtg ctcgccgagc ccgcagccga
    13501 tgggctcgga cggccggtga ccgccatcgc ccaggtcgcc cctggtgcga ccatcgatgc
    13561 tcatctgcac gacggccggc tccgggtgga ggtggtgtcc gtgaacgaag aaagcgcgct
    13621 gcaggcggcg caaacccacg tggaggagca ctagatgctc gcccgcgccg cgctggccac
    13681 gctgtgctgg ctgacgccga tgctcctgcg ggccgacgcg gtgcctggcg gtttgtacgc
    13741 ctacgggccc gtgccgcccg cctcggaggt gcgtttcgac gatcgtcggg ttctggttat
    13801 cgacgagacc gctctcgtgg gcatcccgct gtctgcatca cccggcacgc actcgctgac
    13861 catcacctat gcgtccggag agtcggtctc gcaccagttc gaggtggtgg caaagcagta
    13921 cccggagcag cgcctgacta tcaccgatcg gaaaatggtc aatccggacc cggaggacct
    13981 cgagcgcata cggacagaaa gcgcgaggat gcgcgagcag tacctgagct ttaccgaagc
    14041 catgcagagg ctgcgtccgt tccataagcc ggtgaacgga gtgacctcaa gccccttcgg
    14101 ccgccggcgc atcctcaacg accagccccg caacccgcac agcggtctgg acatcgccgc
    14161 cgaccatggc acccccgtga gcgctccggc acctgcaagg gtcagtctca cgggc
//
LOCUS       DBNHLM010000003         3758 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_299706, whole genome shotgun sequence.
ACCESSION   DBNHLM010000003 DBNHLM010000000
VERSION     DBNHLM010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_299706"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..115)
                     /locus_tag="AC16QF_00070"
     CDS             complement(<1..115)
                     /locus_tag="AC16QF_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR6446973.1"
                     /translation="MSKAKFERTKPHVNVGTIGHVDHGKTTLTAALTKVCAE"
     gene            complement(182..2281)
                     /gene="fusA"
                     /locus_tag="AC16QF_00075"
     CDS             complement(182..2281)
                     /gene="fusA"
                     /locus_tag="AC16QF_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR6446974.1"
                     /translation="MARTTPISRYRNIGIVAHVDAGKTTTTERVLFYTGLSHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTCFWRGMNQQFDEHRINIIDTPGHVDFTIEVERS
                     LRVLDGAVVVFCATSGVEPQSETVWRQANRYEVPRIVFVNKMDRAGADFLRVVDQIKN
                     RLGAAPVPIQLSIGAEEQFKGVVDLIRMKAIIWNEDDQGLTYDLADIPEELMADAEHW
                     REQMVENAAEANEELMEKYLEEGELSAEDIKEGLRIRTLANEIVPALCGSAFKNKGVQ
                     AVLDAVVEYLPAPTEVKAIEGLLQDEETVESRQADDDAPFAALAFKIATDPFVGTLTF
                     FRVYSGVLSSGDQVYNTVKSKRERVGRMVQMHSNNRNEIKEVRAGDIAAAIGLKDVST
                     GDTLCAQDSLITLERMEFPEPVISVAVEPKSVPDQEKMGIALGKLAQEDPSFRVETDE
                     ESGQTIISGMGELHLDIIVDRMRREFGVEANIGKPQVAYRETIRAPIEQEGKFVRQSG
                     GRGQYGHVWLRIEPYEDEEGLATYEFVDEIVGGVVPREYIPAVDKGIREQMQNGVIAG
                     YPLIGVRAILYDGSFHEVDSSEMAFKIAGSMALKEGAQKASPVLLEPIMNVEVVTPED
                     YMGDVVGDLNRRRGIIAGMDENPAGKIIRADVPLSEMFGYSTDLRSATQGRATYTMEF
                     AKYAQVPESIAEGIINR"
     gene            complement(2325..2795)
                     /gene="rpsG"
                     /locus_tag="AC16QF_00080"
     CDS             complement(2325..2795)
                     /gene="rpsG"
                     /locus_tag="AC16QF_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR6446975.1"
                     /translation="MPRRRIVAKREILPDPKYNNQTVAKFINHVMVSGKKAVAERIVY
                     GALSRIEERDNPDPVEIFEKALEAVAPFVEVKSRRVGGATYQVPVEVRASRREALAMR
                     WLVDSARKRGEKSMAARLAGELMDAADGRGSAVRKREDTHRMAEANRAFSHYRY"
     gene            complement(2867..3238)
                     /gene="rpsL"
                     /locus_tag="AC16QF_00085"
     CDS             complement(2867..3238)
                     /gene="rpsL"
                     /locus_tag="AC16QF_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR6446976.1"
                     /translation="MATISQLVRKPRKRKVDKNIVPALQGSPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGYEVTSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGTL
                     DTSGVSDRKQRRSKYGAKRPK"
     gene            complement(3394..>3758)
                     /locus_tag="AC16QF_00090"
     CDS             complement(3394..>3758)
                     /locus_tag="AC16QF_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6446977.1"
                     /translation="RVTEVNEALEAEGKELAKFERLLLGITKASLATESFISAASFQE
                     TTRVLTEAAVTGKQDHLRGLKENVVVGRLIPAGTGLTYHSDRKRKREEELAARLSREQ
                     GVTADDIEQALSEALSSE"
BASE COUNT          637 a         1233 c         1083 g          805 t
ORIGIN      
        1 tctccgcaca caccttcgtc agcgcagccg tcagcgtcgt cttaccatgg tctacatgac
       61 caatcgtccc aacgtttacg tgtggcttag tacgctcaaa tttcgctttg gacatcgtcg
      121 ttaccctatt cttggtggcc gcgggaagcg gcttcttggt ggccgcggga agcggcgaac
      181 tttatcgatt gataatgcct tcggcaatgc tttcgggaac ctgtgcgtac ttggcgaact
      241 ccatcgtgta ggtcgcccgc ccctgggtgg ccgaacgcag gtccgtggag tacccgaaca
      301 tctcggacaa cggcacatcc gcgcggatga tcttgcccgc cgggttctcg tccatgccgg
      361 caatgatccc gcggcgccgg ttcaggtcgc ccaccacatc gcccatgtaa tcctcgggcg
      421 ttacgacctc aacgttcatg atgggttcga gcaggaccgg acttgccttc tgcgcaccct
      481 ccttcagggc catggacccg gcgatcttga acgccatttc cgacgagtcc acctcgtgga
      541 aagagccgtc gtagagtatg gccctgaccc cgatcagcgg gtaaccggcg atcacgccat
      601 tctgcatctg ttcccggata cccttgtcca cggcgggaat gtattcccgc ggcacgacgc
      661 cgcccacgat ctcatcgacg aactcgtagg tggccaagcc ctcctcgtcc tcatagggct
      721 cgatccgcaa ccagacgtga ccatactgac cgcggccacc actctgacgg acgaacttgc
      781 cttcctgctc gatgggcgct cggatggtct cgcgataagc cacctggggc ttgccgatgt
      841 ttgcttcgac cccgaactcg cgacgcatgc gatcgacgat gatgtccaga tgaagttctc
      901 ccatgccgga gatgatcgtc tggcccgact cttcatcggt ttccacgcga aacgacggat
      961 cttcctgagc cagcttgccg agcgcgatgc ccatcttctc ctggtcgggc accgatttgg
     1021 gctcgacggc cacggagatg acgggctccg gaaactccat acgctccagg gtgatcaggc
     1081 tgtcctgggc acagagcgta tcccccgtgc tgacgtcctt cagaccaatg gcagcggcaa
     1141 tgtcgcccgc gcgcacttcc ttgatctcgt tgcggttgtt cgaatgcatc tgcaccattc
     1201 ggcctacccg ctcgcgcttg ctcttgacgg tgttgtagac ctgatcgcca gaactcagca
     1261 cgcccgagta aacgcggaaa aaggtcaacg ttcccacgaa cggatccgtg gcgatcttga
     1321 aagcgagagc cgcaaacggc gcatcgtcat ccgcctgacg cgattccacc gtttcttcgt
     1381 cctggagcag accttcgatg gctttcacct cggtgggtgc gggaaggtac tccaccaccg
     1441 catccagcac cgcctggacg cccttgttct tgaacgcgga gccgcagagg gcggggacga
     1501 tctcgttggc gagcgtgcgt atgcgaagac cctctttgat gtcctcggcc gacagctccc
     1561 cttcctcgag gtacttctcc atcagctcct cgttggcttc ggcagcgttt tccaccatct
     1621 gttcgcgcca gtgttcggca tcggccatca gttcttcggg aatgtccgcc aggtcatagg
     1681 tgaggccctg gtcgtcctcg ttccagatga tggccttcat tcgaatgaga tcgaccacac
     1741 ccttgaactg ctcctccgcg ccgatggaca gctgaatggg cacaggcgcg gcgccgaggc
     1801 ggttcttgat ctgatcgacg acccggagaa agtcggcccc ggcccggtcc atcttgttga
     1861 cgaaaacgat gcgcggcacc tcgtagcggt tggcctggcg ccacaccgtc tccgattgcg
     1921 gctcgacgcc cgaagtggcg cagaacacga ccacggcccc gtcgagaact cgcaggctgc
     1981 gctccacctc gatggtgaag tccacgtgcc cgggcgtatc gatgatgttg atgcggtgct
     2041 cgtcgaactg ctggttcatg cccctccaga agcaggtggt ggccgcggag gtgatggtga
     2101 tgccccgctc ctgctcctgc tccatccagt ccatggtggc cgcgccgtcg tgaacctcgc
     2161 cgatcttgtg cgacagcccc gtatagaaga gcacccgctc cgtggtggtg gtctttcccg
     2221 catccacgtg cgcaacgatg ccgatgttcc ggtatcggga aataggggtc gtacgagcca
     2281 tcatgctctc cagaaattcg aagggaactt aaacaaggga accgctagta acgatagtgc
     2341 gagaacgccc ggttggcttc ggccatgcgg tgagtatcct cccgcttcct gaccgcggag
     2401 ccacgaccgt cggcagcatc cattagctcg ccggcgagac gggcagccat cgacttctct
     2461 ccacgcttgc gcgcactgtc caccagccag cgcatggcca gggcctcgcg tcgggacgcc
     2521 cggacctcga ccggcacctg gtaggtcgca ccccccacgc ggcgggattt cacttcgaca
     2581 aatggcgcca ccgcttcgag agctttctcg aaaatctcga ccgggtcggg gttgtcccgc
     2641 tcttcgatgc gcgacagcgc gccgtagacg atcctctccg caacggcttt cttgccgctc
     2701 accatcacgt ggttgatgaa cttggcgacc gtctggttgt tgtacttggg atcaggaagg
     2761 atttcgcgct tggcgacgat tcgacgtctg ggcattagct gctccttggc ttcaggtgtt
     2821 tccccgaggt tatccggggc cttactggaa cgaatattcc ggcgatttac ttgggccgtt
     2881 tggctccata tttagaacgg cgctgcttgc ggtccgagac acccgaggtg tcgagcgttc
     2941 cgcgaaccgt gtggtagcga acgcctggca ggtccttaac acgacctccg cgaatcagga
     3001 ccacggagtg ctcctgcagg ttgtgccctt caccgccgat gtaagaggtg acttcgtagc
     3061 cgttggtcag gcgaacccga cagacctttc gcagggccga gttcggcttc ttgggcgtgg
     3121 tcgtgtacac gcgcgtgcaa acgccccgct tctgcggtga gccctgcaaa gcggggacga
     3181 tgttcttgtc cacttttcgc tttcgaggct ttcgtaccaa ctggctgatc gtcgccattc
     3241 ttgctccgtt actgactctt ttcccgcaac ccgccgacag ggctggctcc ggggggcgcg
     3301 attgtaatca cacgtcctgc ggttagcaac ggcggcaatc cagcgattgc cgccgccccc
     3361 gcgaccacac ctggggccgc tcctgttgca gggctactcg ctgctcaagg cttccgaaag
     3421 tgcctgctcg atatcgtccg ccgtcacccc ctgctccctc gacagacggg ccgccagctc
     3481 ttcttcgcgc ttgcgcttgc ggtcgctgtg ataggtcagg cccgttccgg ccgggatcag
     3541 ccgtccgacg acgacgtttt ccttcaggcc gcgaaggtga tcctgctttc cggtcactgc
     3601 ggcctcggta agcacccggg tcgtctcctg gaaggaggca gccgagatga acgactccgt
     3661 tgccagagac gctttcgtga tgccgagcag cagccgctcg aacttcgcca gctccttgcc
     3721 ttcggcttcc agcgcctcgt tgacctccgt cacccggt
//
LOCUS       DBNHLM010000004        16005 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_898481, whole genome shotgun sequence.
ACCESSION   DBNHLM010000004 DBNHLM010000000
VERSION     DBNHLM010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 16005)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16005)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16005
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_898481"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="AC16QF_00095"
     CDS             complement(<1..688)
                     /locus_tag="AC16QF_00095"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR6446978.1"
                     /translation="MVAWRLGPAAFAALVWGLTGATSSSLAAAADQAAGAQTLAPPRV
                     VLVLSGGGARAAGHIGVLKALEELRVPVHGIVGTSMGAVVGGLYAAGHSPAGMERLVA
                     RVDWQELLIDDPPRESFRFRRKREDASLFVQAASGVDTEGLKLPKGVVHGHRLKAFLA
                     RHTLHVGDVDDFRQLSIPFASIATDISTGEAVELTGGNLAEAMFASLAIPAFVGPTEI
                     DGRLLVDGGVS"
     gene            complement(682..1101)
                     /locus_tag="AC16QF_00100"
     CDS             complement(682..1101)
                     /locus_tag="AC16QF_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6446979.1"
                     /translation="MKYLHTMVRVTDLDDSLDFYCAKLGLHEVGRHESEAGRFTLVFL
                     AAPGDEEAQVELTYNWDPQEYTGGRNFGHLAYQVEDIYALCQHLMDSGVTINRPPRDG
                     NMAFIRSPDGISVELLQKGGALAPADPWKDMPNVGEW"
     gene            complement(1511..1711)
                     /locus_tag="AC16QF_00105"
     CDS             complement(1511..1711)
                     /locus_tag="AC16QF_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6446980.1"
                     /translation="MKNFRIRPLHRQRNGRRQRVRVLDQNELADLLGVPITELRKELD
                     DRGWSYHEDSTGNLWATRPERP"
     gene            complement(1773..3164)
                     /locus_tag="AC16QF_00110"
     CDS             complement(1773..3164)
                     /locus_tag="AC16QF_00110"
                     /inference="COORDINATES: protein motif:HMM:NF023222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6446981.1"
                     /translation="MMTGDEYRASLNDGRETFFEGKQVDDLPNHPILGITVESAASGY
                     DRFYDPAPDAVGAFMKVPGSAVELREQVELHETVDLLTHVTYASIMTLLTAADRIEAQ
                     LPDNARRIREYVRDVQRKDLRITQCITDAKGDRSRPPGKQDDPDAYVRVIERRADGVV
                     IRGAKLHISAASMGHELMTIPTKSMKPGEDDYAVACMVPVNAPGVKIVNTTYAPRHED
                     KRDFPISGSHHTPEGFVIFDDVFVPDERVFLDGPPEFAAVFAHSLGLWERLGGLASMA
                     SSYDRLVGFAQLICEANGLERTGHIKEKISEMMINATLIRASLEAAIDNCSITSDGAA
                     FPNELYTNVGKYHGAANWSAMVRHLHDIAGGSILTAPGMADLENEKTGHLIRKYMGTT
                     QEVDGVYRTRLFHAIRDMTADALGGWNAVTNIQAGGGLYAQRIVSRMHYDIDKAKRMA
                     LESAHMQEYLSDA"
     gene            complement(3209..3895)
                     /locus_tag="AC16QF_00115"
     CDS             complement(3209..3895)
                     /locus_tag="AC16QF_00115"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6446982.1"
                     /translation="MTGRRLTGKRVLITQADDYMGPATAELFASHGAQIIADARDLRP
                     AGACEALVHEAGRVDVLIANLAGPNFSGTPATAIDDEAWATVFDTMVHPLHRLTRAVL
                     PQMQDRQSGKIIVYGSATALRGLKTVAAYSAARAAQVGYVQAVGVEVAPLNVQVNLIA
                     QNYVENPVYYPPELTAKESFQRSLKRQVPLGRLATAREDAQFALFLASDESDFFVGQA
                     IPFSGGWVQR"
     gene            4072..4929
                     /locus_tag="AC16QF_00120"
     CDS             4072..4929
                     /locus_tag="AC16QF_00120"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6446983.1"
                     /translation="MLISGVSQGCVYGLIALGFVLIYKATEMVNFAQGDVMMLGAFLA
                     FALINLMELPFLWGFMGALAAMAVVGVLLERLLLRPMIGEPHFAVLMLTIGLGFILRA
                     VAGAIWGNEPRALHTPYGGEVLRFGDLVVGYENIVIIVGTAVLCGLLFAFFRFTRVGV
                     AMQAASQNQLAAYYVGIPVKRIYSLVWALSATIAACAGVLIAPVTLIDPLMGSFLGIK
                     AFAAAIVGGFGSLPGAILGGLLVGITEQFVGLYFPPGFADVSAYVLLLVMLMIRPEGL
                     FATMQRKKV"
     gene            4934..6010
                     /locus_tag="AC16QF_00125"
     CDS             4934..6010
                     /locus_tag="AC16QF_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6446984.1"
                     /translation="MRFIRKTHYVQDVDLIKHPGQRFWYGLLAIVLVLAPLGLDRFYL
                     GELSLVFIYAIAGIGLMLLVGYTGLVSLGHAAFLAVGAYCHSWLLGNGVPLPLAMVAA
                     TLVTAGVGAVVGVPALRMTGIYLAVATLALAVIVEKVLEKWETVTGGLRGLPVPKAQF
                     LGFDLGAPANFYYLCLGVLALGLLAAMNLLRSPTGRAMVAVRDSETSAQSMGIHINRI
                     KGLAFALSAGFTGLAGALFAHRIGYLFPDSFTVLTSIQLLLMVVVGGLGSLHGVVFGA
                     IFIGLLPQGIALFRDVVPPAIAQIPGLEPGIFGLILVLFLIFEPLGIYGRWVKIRLFF
                     EEFPLYRRATYKRQKTYLRTERLR"
     gene            6007..6765
                     /locus_tag="AC16QF_00130"
     CDS             6007..6765
                     /locus_tag="AC16QF_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6446985.1"
                     /translation="MSLFAAEDVAVSFGGIKAVHDLSFAVEDGEVFSIVGPNGAGKTT
                     IFNLISRIYDVDQGSLAFDGTEITRLPAHAVAELGVARTFQNTELFEQETVLKNLLIG
                     CHVHRRSGLLADILFLPSVKAQELTFRKRVEDVIDLLDLQSYRDQVIGNLPYGVRKMV
                     EIARALCLGPKLLLLDEPSSGLNPEETEDLSFWLEDINNDLGITIIMVEHDMNLVSRA
                     SDRVMALADGALLTIGSPADVQQHPDVLRAYLGD"
     gene            6777..7535
                     /locus_tag="AC16QF_00135"
     CDS             6777..7535
                     /locus_tag="AC16QF_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6446986.1"
                     /translation="MLELKNVETYYGPIMAIRGVSMEVPEGKIVSLLGANGAGKTTVL
                     KTISGAMDPQKGTITYQGEPIQARDPDWVARRGIAHVPEGREVFPFLCVEDNLRMGAF
                     SRRDAEIGDDIELVYGYFPVLKELARQQAEYLSGGQQQMLAIGRALMLRPRLMLLDEP
                     SLGLSPRLVAEIAEIIVRLNREQAVTMLLVEQNAKMALDIGDYGYVMEIGRIVMEDTC
                     ERLKNATDIKEFYLGMKDEGVRGRRRWKQRKTWR"
     gene            7532..9346
                     /locus_tag="AC16QF_00140"
     CDS             7532..9346
                     /locus_tag="AC16QF_00140"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR6446987.1"
                     /translation="MSPQIIDGCATVARLFWKRATDLPDKIALREKDFGIWNEYTWGD
                     WGEQARLVGLGLKALGLKRGDVCSIATDVNKEWLFADHGVIGVGGIVNGVYPTDAPNQ
                     VEYLINDSRTRFYFAEDEEQLDKVLEVRGNTPTLEKIIIFDMEGLRTFSDEQCMSFDA
                     LLALGREYGEQHPGLWEQEIEAAEAEDLLILTYTSGTTGNPKGAMISNRNMIFMMRNL
                     QRMYGITSADEQLGFLPTAHVAAQMFYNFAVIESCSVINLVEELETTDRDQQEIAPTI
                     HFAVPRVWEKKHSLVTIKLKEGTVLGRWAYDLAMRIATPLAHARQRGETPSWISRLLY
                     RAVDPLVLKNIRVLLGIDRCRWLSTGAAPIAPELIDWYWALGRPMFEVYGQTECTGLA
                     TANHPGEVRIGSVGKPGQEIEVTLSEAGEILIRSPGVICGYWENPEKTAETFRQGWLH
                     TGDVGRIDEDGFVYVVDRMKDIIITAGGKNITPSEIENQLKFSPYITDAVVVGDRRPY
                     LTCLIMIDHENVTKYAQDHDVPFTNYTSLCHAREVQDLVEAEIEKVNARFARVETIKK
                     FRLIDQLLDEEDDELTPTMKLKRKVVHEKYGELIDTMY"
     gene            9375..10535
                     /locus_tag="AC16QF_00145"
     CDS             9375..10535
                     /locus_tag="AC16QF_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6446988.1"
                     /translation="MENVYRNGLMAILSLLLLGACGGEATQTNEVVFGVHTDLSGPTA
                     IWGVGATNGARMRFDEANAAGGVHGRSIRYVVEDAGYQVPNAIRAANKLINRDQVLAM
                     VLAVGTPMNNAIMMEQFEQGVPNLFPISGARSMVEPLHPLKFTQRGIYYYEVRAAIRY
                     FVDSQGKQSVCAIYHDTDYGHEILEAVNDQLAEMNMALVESAAHKPSESEFTAAILRL
                     RNAGCDLVLMGTVHRDTILVLDSARKMGWEDVAWVGNNAAYGQVIADSPSCEGYYAFV
                     HMAKIYADDEKTPAVQAWWDHYVALYDEEPGLAAMEGYRAADLLVLALEKAGPDLSVG
                     SLLAALEGITDYTDLFGYNVSFGPEKHSGATESVLSQVQGGRWVTLAESISY"
     gene            complement(10547..12529)
                     /locus_tag="AC16QF_00150"
     CDS             complement(10547..12529)
                     /locus_tag="AC16QF_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HIR6446989.1"
                     /translation="MQSGDKAYAELDRPVFLTSASLLAAVIFLAALFPDTMDALFSDV
                     QGAIVAGASWYYVLVVAVILVSVLVFAFSSYGQIKLGPDHSEPDYSFISWFAMMFAAG
                     MGIGLMFFGVAEPVMHFLSPPKGDPGTIAAAREAMKLTFFHWGLHGWAIYAIVALILA
                     YFSFRHGLPLTLRSALYPLIGERIYGPIGTAVDVFAIISTTFGVATSLGLGVNQINAG
                     LNYLFGLPVTQEIRVGLIIVTTAIATVSVVLGLDRGIKRLSEINIVLAVMLMITVLAI
                     GPTTLLLQAFMQNTGDYLADLVGKTFNLYAYDPTDWLGGWTIFYWGWWISWAPFVGLF
                     IARISRGRTLREFVIGVLIGPVVFTFLWMTVFGNSAIDLIMNQGVTELGSAVQANESI
                     ALFRFLEQFPATGFLSALAIVMVVVFFVTSADSGALVLNMLSSNGSDQTPVGRRIFWM
                     SAIGVTAIVLLLVGGLSALQTAVIASALPFSVAVMAGIWGFSKALYVDHAKRVSHAAA
                     TVPPILARPEISWQHRLTQLLRHPQDAEVRAFMRERVMPVMREVGDELKRHGLDVEVI
                     VDGGEGESLRLEIAHGEEIDFVYEVVNRKHPLPDEALVGTAMKDMPPSDTYHRAEVHL
                     REGSQDYDIMGWTSEQIAHDITEQYEKHRHFLHMLR"
     gene            complement(12585..14585)
                     /locus_tag="AC16QF_00155"
     CDS             complement(12585..14585)
                     /locus_tag="AC16QF_00155"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     sulfotransferase family protein"
                     /protein_id="HIR6446990.1"
                     /translation="MSVASSGPGPDHREPSLASARRALEQGQFDAAVQQAEALLGRGD
                     ADTSAARYLLAVACRYAGDCERAEAALQELLASEPRHARAWQERGYVALKRNDPNAAR
                     EYFRHAVTHNSALVASWRSLVKLHGAAGERELAEQAARRLDELSALPREILAAASHLN
                     EDRLYKAEQICRDYLQENPTHIPAMRLLAEIGVRLQVLDDAEFLLESALEFEPDHELA
                     RADYANVLIRRQKFTKALEALRPLLLQRPDHVPYRIAEAMATTGSGDHDAAITQYRQI
                     LASHPDLPQLHVSLGHAQKTIGDIDGAVASYQQAYGVRRDFGDAYWSLANTKTYRFTP
                     DEIDGMRQAEADPTCGTQDRIHLCFAAGKAFEDAGSYQESFEYYERGNRLKQGEIRYD
                     AGQIDRRVEAQMAVCTRELFARQEGHGDPAPDPIFVVGLPRAGSTLLEQILASHSMVD
                     GTMELPSLLAIAQRLRGRSSVRSDRYPRILGELKPEQLHQLGAHYLQETLPYRQGAPR
                     FVDKMPNNFFHVGLIRLLLPNARVIDARRHPMACCFSGFKQLFGEGQEFSYGLTEIGH
                     YYRRYVELMKHWDEVLPGFVLRVQHEDVVDDLEGQVRRILEFCELPFEDACVNFHRTE
                     RAIRTPSSEQVRQPIYRDGLDQWQRFEAWLDPLKAALGDALG"
     gene            complement(14602..>16005)
                     /locus_tag="AC16QF_00160"
     CDS             complement(14602..>16005)
                     /locus_tag="AC16QF_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6446991.1"
                     /translation="GANAGNVQFGPNTTFVNDFTRDQEQLAFFGEFAFDLTDAVTLSV
                     GARYYEIDFDFGGASNFSFGCKFRDGTDEFPDGPLNPDGSCNGTGYSNDVSERLEVLG
                     TGDADLIGAALSPSGQRPLFRGTNNPQLLLDALAAGTFDIDDLNSDGVVKIDDTIFRF
                     ALDWRLNDQIMLFSTFSEGFRPPASNRNAGQASANQTGPFEGFLIPAYAETDELQNYE
                     LGMKGDFLDRTLRFNATAYYSEISDLQTARFDPSNVAFLVFLENVGDAEIKGFDADFT
                     WLATDRLTVSGAFSIVDTEITRLNPQLQGIAVPEGSELPFAPNFSGNIRARYQFPLDA
                     FEANGYVQLGVSYRGESLSGITGNAFLAEQVHQEVYGVGSGLKIKSEGGTYSSPIPAT
                     GETFENGRYKQESYALVDLAVGVQKNGWTAEFFVKNLADESAQIHITTSNSADFTPKV
                     VTNRPRSFGLRVSYDHF"
BASE COUNT         2754 a         5039 c         5292 g         2920 t
ORIGIN      
        1 tggaaacgcc gccgtccacg aggagacgcc catcgatctc cgtggggccg acgaatgcgg
       61 gtatggcgag gctggcgaac atcgcttcgg cgagattgcc cccggtgagc tccacggcct
      121 ccccggtgct gatgtccgtg gcgatgctgg cgaacggtat gctcagctgg cggaagtcgt
      181 cgacgtcccc gacgtgcagg gtgtgccggg cgagaaacgc cttcaggcgg tggccgtgaa
      241 ccacgccctt gggaagcttc agaccttccg tatcgacgcc ggacgcggcc tggacgaaaa
      301 gactcgcatc ttcgcgcttg cgtcggaaac ggaaggattc ccggggcgga tcgtcgatga
      361 gcagttcctg ccaatccaca cgggcaacga gccgttccat tcccgccggc gagtgaccgg
      421 ccgcgtagag gccgccgacc acggcgccca tgctggtgcc gacgatgccg tgaacgggga
      481 cccgcaactc ctcgagcgct ttgagcacgc cgatgtgccc ggcggcccgg gcgccgccgc
      541 cgctgagcac cagcaccacc ctaggtgggg cgagcgtctg cgctcctgcg gcctggtcgg
      601 cggcagcagc cagggatgat gaggttgcgc ccgtgaggcc ccagaccagc gcagcaaagg
      661 ccgcgggccc gaggcgccac gctaccattc gccgacgttc ggcatgtcct tccagggatc
      721 ggcgggcgcc agagccccgc ccttctgcag cagctctacc gagatgccat cgggtgaccg
      781 gatgaaagcc atgttcccgt cacgtggcgg gcggttgatg gtgacgcccg agtccatcag
      841 gtgctggcac agcgcgtaga tgtcttcgac ctggtaggcg aggtgcccga agttacggcc
      901 gcccgtgtac tcctgcgggt cccagttgta ggtcagctcc acctgtgctt cctcgtcgcc
      961 tggagcggcc aggaagacga gcgtgaaacg ccccgcctcg ctttcgtgcc gaccgacctc
     1021 gtgaaggccg agcttcgcgc agtaaaagtc gagtgagtcg tccaggtcag tcacccggac
     1081 cattgtgtgc agatatttca tggaatgttt tcctcgcccg gatcagccgg ggcgcgtgca
     1141 tcataccgcc cggtttcttc agggcaaaaa aaagccccgc cgaagcgggg ctagccattt
     1201 ggtcatcaca aggtctatgg agagtgtgtt gctcttcagc tagagggtta cgaatgcagc
     1261 caggctcagc atcagggtga gcagagcgcc gaggagtagt gcgctctcac ctagggtcag
     1321 ctgcccctgg tccctgtgtg cggctatcgc tgccgcactg gacatttgcg tatggggttc
     1381 gttacgcaga tctcgcatcc gctccggccc gttcaaaaca acacgataga ggttaggcgg
     1441 taactctgca gccaggacat aaggattctg gaatgaatat ggagggttca aaagggcgga
     1501 aacgcccgtc tcatgggcgt tctgggcggg tcgcccacag gttgccggtg ctgtcctcat
     1561 ggtaggacca accccgatcg tccagttcct tgcgcaattc ggtaatcggc accccgagca
     1621 ggtcggccag ctcgttctgg tcgagcaccc gcactctttg ccggcggccg ttgcgctgac
     1681 ggtgcagcgg ccgaatcctg aaattcttca tccgctgtgc tgcgcggctc ggtcccaata
     1741 actgtatata catacagtat gcggcgcaac gatcaggcgt cgctgagata ctcctgcatg
     1801 tgggcggact ccagcgccat ccgcttggcc ttgtcgatgt cgtagtgcat gcgcgacacg
     1861 atccgttgcg cgtagaggcc gccgccggcc tggatgttgg tcacggcgtt ccacccgccc
     1921 agcgcgtcgg cggtcatgtc gcgaatggcg tggaagagcc gggtgcggta aacgccgtcc
     1981 acctcctgcg tcgtgcccat gtacttgcgg atcagatgtc ccgtcttctc gttctccagg
     2041 tcggccatgc ccggcgcggt gaggatgctg ccaccggcga tgtcgtggag atgacggacc
     2101 atggcggacc agttggcggc cccgtgatac tttcccacgt tggtgtaaag ctcgttcggg
     2161 aacgccgcgc cgtccgaggt gatgctgcag ttgtcgatgg cggcctcgag gctcgcgcgg
     2221 atgagggtgg cgttgatcat catctcggag atcttctcct tgatgtgccc cgtgcgctcc
     2281 agcccgtttg cctcgcagat gagctgcgca aatcccacca gccggtcgta gctcgacgcc
     2341 atagacgcca ggccgccgag ccgttcccac aggcccaggg agtgggcgaa cacggcggcg
     2401 aactcggggg ggccgtccag gaacacccgc tcgtcgggca cgaacacgtc gtcgaagatc
     2461 acgaagccct cgggcgtgtg gtgtgatccg gagatcggaa agtcgcgctt gtcctcgtgt
     2521 cgtggggcat aggtggtgtt cacgatctta acccccggcg cgttgacggg aaccatgcat
     2581 gccacggcgt agtcgtcctc gccgggcttc atggacttgg tcgggatggt catcagctcg
     2641 tggcccatgg aggctgccga gatgtgcagc ttggcccccc ggatgaccac gccgtcggcc
     2701 cgtcgctcga tgacgcgcac gtaggcgtcg gggtcgtcct gcttgcccgg tggccggctg
     2761 cggtcgccct tggcgtcggt gatgcactgg gtgatgcgca ggtctttgcg ctgaacgtcg
     2821 cggacgtact cgcggatgcg ccgcgcgtta tcgggcagct gcgcttcgat ccggtccgcc
     2881 gcggtcagca gcgtcatgat ggaggcgtag gtgacgtggg tcagcagatc cacggtctcg
     2941 tggagctcca cctgctcgcg cagctcgacc gcggagccgg gcaccttcat gaaggcgcca
     3001 accgcgtccg gggcgggatc gtagaagcgg tcgtagccgc tggccgctga ctccacggtg
     3061 atcccgagga tcgggtggtt gggcaggtcg tccacctgct taccctcgaa gaacgtctcg
     3121 cgcccgtcgt tcagtgaggc gcgatactcg tcgccggtca tcatctggca atccccctca
     3181 agcccaacgc gaaggatggt aaatgagcct agcgttgaac ccaaccgccg ctgaagggaa
     3241 tggcctggcc gacgaagaag tcgctctcgt cgctggccag gaacagcgcg aactgcgcat
     3301 cctcccgggc ggtggccagc ctgccgagcg gcacctggcg cttgagtgat cgctggaacg
     3361 actccttggc cgtcagctcc ggcgggtagt agacggggtt ctccacgtag ttctgggcga
     3421 tgaggttgac ctggacgttg agcggcgcaa cctccacgcc caccgcctgc acgtagccca
     3481 cctgggcggc gcgggcggcg ctgtaggcgg cgacggtctt cagcccccgc agcgccgtgg
     3541 cgctcccgta gacgatgatc ttgccggatt ggcggtcctg catctgcggc agaaccgcgc
     3601 gggtcaggcg atgcaggggg tgaaccatgg tgtcgaagac ggttgcccag gcctcgtcgt
     3661 cgatggccgt ggcgggcgtg ccgctgaagt tgggcccggc caggttggcg atcagcacgt
     3721 cgacccggcc ggcctcgtgg acaagggcct cgcacgcgcc cgccggtctg aggtcccggg
     3781 cgtcggcgat gatctgcgcg ccgtggctgg cgaacagctc ggccgtggct ggccccatgt
     3841 agtcgtcggc ctgggtgatg agcacccgtt tgcccgtgag tcgccgtccg gtcattttcc
     3901 tcgctccgct gcctgatctc cgggtgtgcc gtccattctg cccggagcgg gtcctggcgg
     3961 gaatggcgct ggcacggatc cgagatgaca aagcagccgt cgattcggac aataggcgtc
     4021 cgaggggatc aaaaaccaaa acgcgccagc caagacgtgg aagttctgca gttgctaatc
     4081 agcggagtgt cgcaggggtg cgtctatgga ttgatcgccc tggggttcgt gctgatctac
     4141 aaggccacgg agatggtcaa tttcgcccag ggcgacgtca tgatgctcgg cgcgttcctg
     4201 gccttcgccc tgatcaacct catggagctt ccctttctgt ggggcttcat gggcgcgctc
     4261 gcggccatgg ccgtggtggg ggtgctgctc gagcggctgc tgctgcgtcc gatgatcggc
     4321 gagccgcact tcgccgtgct catgctcacc atcgggctcg gtttcatcct gcgcgccgtg
     4381 gccggcgcca tctggggcaa cgagcctcgt gccctgcaca cgccgtatgg gggagaagtg
     4441 ctgcgcttcg gtgacctggt ggtcggttac gagaacatcg tgatcatcgt cggcaccgcc
     4501 gtgctgtgcg gcctgctttt cgccttcttc cgctttaccc gggtgggtgt cgccatgcag
     4561 gccgcttccc agaaccagct cgccgcttac tacgtgggca ttccggtcaa gcgcatctac
     4621 tcccttgtct gggctctgtc ggcgaccatc gccgcgtgcg cgggcgtact catcgcgccc
     4681 gtcacgctga ttgaccctct gatggggagc ttcctgggca tcaaagcgtt cgcggcggcg
     4741 atcgttggcg gtttcggcag ccttcccggc gccattctag gcggtcttct ggtgggcatc
     4801 acggagcagt tcgtcgggct ctattttccg ccgggattcg ccgatgtgag cgcctacgtg
     4861 ctgctgctcg tcatgctcat gatccggccc gaagggctct tcgcgaccat gcagcgcaag
     4921 aaggtctgag gccgtgcgtt tcatccgcaa gacccactac gttcaggacg tcgacctgat
     4981 caagcatccg gggcagcgct tctggtacgg gctgctcgcc atcgttctcg tcctcgcgcc
     5041 cctggggctc gaccggttct acctgggcga gctgtctctc gttttcatct acgccatcgc
     5101 cggcatcggg ctgatgctgc tggtggggta cacgggcctc gtgagcctgg gccatgcggc
     5161 ctttctggcc gtgggcgcgt actgccattc gtggctgctg ggcaacggcg tgcctctgcc
     5221 cctggcaatg gttgcggcga ccctggtcac cgccggcgtc ggcgcggtgg tcggcgtgcc
     5281 cgccctgcgg atgacgggca tttacctggc cgtggcgacc ctcgccctgg ccgtgatcgt
     5341 cgagaaggtg ctggagaaat gggagacggt gaccggcggc ctgcgtggcc tgcccgtgcc
     5401 caaggctcag tttctcggct tcgacctcgg cgctccggcc aacttctact atctgtgtct
     5461 tggggtgctt gcgctcggtc tgctggcggc gatgaacctg ctgcgcagcc ctacgggccg
     5521 ggccatggtc gccgtgcggg actccgaaac ctcggcccag agcatgggca ttcacatcaa
     5581 ccgcatcaag gggctggcct tcgccctgtc ggccggcttc acgggtcttg cgggcgcgct
     5641 gtttgcgcac cggattggct acctctttcc ggactcgttc accgtgctca cctccattca
     5701 gctgctgctg atggtcgtcg tgggcggcct cggctcgctg cacggggtcg tgttcggggc
     5761 catcttcatc ggcctgctgc cccaggggat cgccctgttc agggacgtgg tgccgccggc
     5821 gatcgcccag ataccggggc tggagcccgg catattcggc ctcattctcg tgctcttcct
     5881 gatattcgaa ccgctgggta tatacggccg ctgggtcaag atccgccttt tcttcgagga
     5941 gtttccgctc tatcggcggg cgacctacaa gcggcagaag acgtatctgc gcacggagcg
     6001 attgcggtga gcctttttgc cgcggaggac gtcgcggtga gcttcggcgg catcaaggcc
     6061 gtgcacgacc tttcctttgc cgtggaagac ggcgaggtgt tcagcatcgt aggccccaac
     6121 ggcgcgggca aaacgacgat cttcaacctc atcagccgaa tctacgacgt ggaccagggg
     6181 tcccttgcct tcgacggcac agagatcacc aggttgccgg cccacgccgt tgccgagctc
     6241 ggcgtcgcgc gcacgttcca gaacaccgag ctgttcgagc aggaaacggt gctgaaaaat
     6301 ctgctgatcg gctgtcacgt ccatcgacgt tccggcctgc ttgccgacat tctctttctg
     6361 ccgtccgtga aggcccagga gctgactttt cgcaagcggg tggaggacgt catcgatctg
     6421 ctggatctgc agagctaccg tgatcaggtg atcggcaatc ttccctatgg cgttcgcaag
     6481 atggtggaga tcgccagggc gctgtgcctg ggcccgaagc tgctgctgct ggacgagcct
     6541 tcgtccggtc tcaacccgga ggagaccgag gacctctcct tctggctgga ggacatcaac
     6601 aacgatctcg gcatcaccat catcatggtg gagcacgaca tgaatctcgt ctcccgcgct
     6661 tcggaccggg tcatggcgct ggcggacggg gcgctgctga ccatcgggtc tccggccgac
     6721 gtccagcagc acccggacgt cctgcgggcg tacctggggg attgaccgtg gacgcgatgc
     6781 tcgagctcaa gaacgtggag acctattacg gccccatcat ggccatccgc ggcgtcagca
     6841 tggaggttcc cgagggcaag atcgtctccc tgctcggtgc caacggcgcc ggcaagacca
     6901 ccgtgctcaa gaccatcagc ggcgccatgg atcctcagaa gggcaccatc acctaccagg
     6961 gcgagccgat ccaggctcgt gacccggact gggtggcgcg ccgcggcatc gcgcacgtcc
     7021 cggaaggccg cgaggtcttc ccctttctct gcgttgagga caacctgcgc atgggcgcgt
     7081 tctcccgccg ggacgccgag atcggcgacg acatcgagct ggtgtacggc tattttcccg
     7141 ttctcaagga gctcgcgagg cagcaggccg agtacctgtc cggcgggcag cagcagatgc
     7201 tcgccatcgg cagggcgctc atgctgcggc cgcggctgat gctgctcgat gagccgtcac
     7261 tcgggctgtc gccccggctt gtcgcggaga tcgcagagat catcgtccgg ctcaatcgcg
     7321 agcaggccgt gaccatgctg ctggtcgagc agaacgcgaa gatggccctg gacatcggcg
     7381 actacggcta cgtcatggag atcggccgca tcgtcatgga agacacctgt gagcggctca
     7441 agaacgccac ggacatcaag gagttctacc tcggcatgaa agacgagggc gtgcgtggcc
     7501 ggcgccgctg gaagcagagg aagacttggc gatgagcccg cagatcatcg acggctgcgc
     7561 gacggttgcc cggctcttct ggaagcgcgc cacggacctg cccgacaaga tcgccctgcg
     7621 cgagaaggac ttcgggatct ggaacgagta cacctggggc gactggggcg agcaggcccg
     7681 gctggtgggt ctggggttga aagccctggg cctgaagcgg ggcgacgtct gctccatcgc
     7741 gaccgacgtc aacaaggagt ggcttttcgc cgatcacggc gtgatcggcg tgggcggcat
     7801 cgtcaacggc gtctacccca ccgatgcgcc gaaccaggtc gagtatctga tcaacgacag
     7861 ccggacgcgt ttctattttg ccgaggacga ggagcagctc gacaaggtgc tcgaggtccg
     7921 cggcaacacg ccgacgctgg agaagatcat catcttcgac atggaggggc tgcgcacgtt
     7981 ctccgacgag cagtgcatga gcttcgatgc gctgctcgcg ctcggtcgcg agtacggcga
     8041 gcagcacccc gggctgtggg agcaggagat cgaggctgcc gaggcggagg acctgctcat
     8101 cctgacctac acctccggga ccacgggcaa tcccaagggc gcgatgatca gtaaccgcaa
     8161 catgatcttc atgatgcgga accttcagcg catgtacggc attacctcgg ccgacgagca
     8221 gctggggttc ctgcccacgg ctcatgtggc ggcccagatg ttctacaact tcgcggtgat
     8281 cgagtcgtgc tccgtgatca atctggtcga ggagctcgag accacggaca gggatcagca
     8341 ggagatcgcg cctaccattc atttcgcggt gccgcgcgtc tgggagaaga agcactcgct
     8401 ggtgacgatc aagctcaagg aggggaccgt gctggggcgg tgggcctacg atctcgccat
     8461 gcgcatcgca acgcccctgg cccatgcccg ccagcgtggc gaaaccccgt cttggatatc
     8521 gcgcctgttg taccgcgccg tcgatcccct ggtgctcaag aacatccgcg tgctgctcgg
     8581 catcgaccgc tgccgttggc tctccaccgg cgcagcaccc atcgcgccgg agctgatcga
     8641 ctggtactgg gcgctgggaa gacccatgtt cgaggtgtac gggcagaccg aatgcacggg
     8701 tctggctacc gccaaccacc ccggcgaggt gcgcatcggc agcgtcggca agcccgggca
     8761 ggagatcgag gtgacgttgt cggaggcggg cgagatactg atccgcagcc cgggcgtcat
     8821 ctgcgggtac tgggaaaacc ccgaaaagac cgccgagacg ttccggcagg ggtggcttca
     8881 caccggagac gtagggcgca tcgacgagga cgggttcgtc tacgtcgtcg accggatgaa
     8941 ggacatcatc atcaccgccg gcggcaagaa catcacgccg agcgagatcg agaaccagct
     9001 gaagttctcg ccctacatca cagacgcggt ggtggtggga gatcgtcgtc cttacctgac
     9061 ctgcctgatc atgatcgatc acgagaacgt gacgaagtat gctcaggacc atgacgtgcc
     9121 gttcaccaac tatacgagcc tgtgccacgc acgcgaggtt caggatctcg tggaggccga
     9181 gatagagaag gtgaacgcgc ggttcgcgcg ggtggagacc atcaagaagt tccggctcat
     9241 cgaccagctg ctcgacgagg aagacgacga gctgacgccg acgatgaagc tgaagcgcaa
     9301 ggtggtgcac gagaagtatg gcgagctcat cgacaccatg tactgacgcc cgcacgggca
     9361 cactggagag ctgaatggaa aacgtctatc gcaacggcct catggcgatc ttaagtctgc
     9421 tcctgcttgg ggcgtgcggt ggagaggcga cccaaacgaa cgaggtcgtg ttcggcgtgc
     9481 acaccgacct ctccggcccc acggcgatct ggggagtcgg tgccaccaac ggcgcacgga
     9541 tgcgcttcga cgaggcgaac gccgccgggg gcgtgcatgg ccgcagcatc cgctacgtgg
     9601 tggaggacgc cggctatcag gttcccaacg ccatacgggc cgccaacaag ctcatcaacc
     9661 gtgaccaggt gctggccatg gtgctcgcgg ttggaacgcc gatgaacaac gccatcatga
     9721 tggagcagtt cgagcagggc gtgccgaacc tctttcccat cagcggggcg cgctcgatgg
     9781 tggagccgct gcaccctttg aagttcaccc agcggggcat ctactactac gaggtgcgag
     9841 cggccattcg ttacttcgtc gacagccagg gcaagcagtc ggtctgcgcg atctatcacg
     9901 acaccgacta cgggcacgag attctcgagg cggtcaacga ccagctcgca gagatgaaca
     9961 tggccctcgt cgagtccgcg gcgcacaagc cctcggagag cgagttcacc gccgccatcc
    10021 tgcggctgcg caatgccggc tgcgacctcg tcctgatggg aaccgtgcac cgcgacacca
    10081 ttctcgtgct cgacagcgcg cgcaagatgg gctgggaaga cgtcgcctgg gtcggcaaca
    10141 acgcagccta cgggcaggtc atcgccgact cgccgagctg cgaaggctat tacgcgttcg
    10201 tccacatggc gaagatttac gccgatgacg agaaaacgcc cgccgtgcag gcgtggtggg
    10261 atcattacgt ggcgctgtac gacgaggaac cgggtctggc tgccatggaa ggctatcggg
    10321 cggcagatct gcttgtgctc gccctggaga aggcgggtcc cgatctcagc gtagggagcc
    10381 tccttgccgc gttagagggc atcacggact acacggacct gtttggctac aacgtatctt
    10441 tcggtccgga gaagcatagc ggcgccacgg agtccgtgct ttctcaggtc cagggcggtc
    10501 gctgggtcac gctcgccgag tccatttcct actaggctgc cgccgatcag cgcagcatgt
    10561 gcaggaagtg ccggtgcttc tcgtactgct cggtgatgtc gtgagcgatc tgctcgctcg
    10621 tccagcccat gatgtcgtag tcctggcttc cttctctcag gtgcacctcg gcccggtgat
    10681 aagtgtccga tggcggcatg tccttcatgg cggtgccgac cagggcctcg tccggaagcg
    10741 gatgcttccg gttcaccacc tcgtagacga agtcgatttc ctcgccgtgg gcaatctcca
    10801 ggcgtaacga ctcgccctcg ccgccatcca cgatcacctc cacatcgagc ccgtggcgct
    10861 tgagctcgtc gccgacctcg cgcatcacgg gcatgacccg ctcgcgcatg aaggccctca
    10921 cttcggcgtc ctgggggtgg cgcagcagct gtgtcagtcg atgctgccat gagatctcgg
    10981 gcctggcgag aatgggcggc acggtcgctg ccgcatggga cacgcgtttg gcgtgatcga
    11041 cgtagagcgc cttgctgaac ccccagattc cggccatgac ggccacggag aagggcaggg
    11101 cgcttgcgat gacggcggtc tgcagcgcgg acaacccgcc caccagcagc agcacgatgg
    11161 ccgtcacccc tatggcgctc atccagaaga tccgccggcc caccggtgtc tgatccgagc
    11221 cgttggatga cagcatgttc agaaccaggg cccccgagtc cgccgaggtc acgaaaaaga
    11281 ccacgaccat cacgatggcc agggcagaca gaaatccggt ggcaggaaac tgctccaaga
    11341 agcgaaacag ggcgatggac tcgttggcct gcacggccga gccgagctcc gtcacgccct
    11401 ggttcatgat cagatcgatg gcgctgttgc cgaacacggt catccacagg aaggtgaaga
    11461 cgacgggacc gatcagcacg ccgatcacga actcccgcag ggtcctgccc cgggagatcc
    11521 gcgcgatgaa gagccccacg aagggcgccc agctgatcca ccagccccag tagaagatgg
    11581 tccatccccc cagccagtcc gtggggtcgt aggcgtagag gttgaacgtc tttcccacca
    11641 ggtcggccag gtagtcgccg gtgttctgca tgaacgcctg gaggagcagg gtggtcgggc
    11701 cgatggccag cacggtgatc atcagcatga cggccaggac gatgttgatc tcggacagcc
    11761 gcttgatgcc ccggtccagg ccgagaacca cggagacggt tgcaatggcc gtggtgacga
    11821 tgatcagacc gacacgtatc tcctgggtca ccggcagacc gaaaaggtag ttcagccccg
    11881 cgttgatctg gtttacgccg agcccgaggg aggtggccac gccgaaggtg gtgctgatga
    11941 tggcgaagac gtcgacggcg gtcccgatgg gcccgtagat gcgctcgccg atcagcgggt
    12001 acagggccga gcgcagcgtg agcggcagac cgtgccggaa gctgaaatac gccaggatca
    12061 gcgccacgat cgcgtagatg gcccagccgt ggagacccca gtggaagaag gtgagcttca
    12121 tcgcctcccg ggcggctgca atggtgcccg ggtctccttt cgggggcgag agaaagtgca
    12181 tcaccggctc ggcgacgccg aagaacatca gcccgatgcc catgccggcg gcgaacatca
    12241 tggcaaacca ggagatgaag gaataatcgg gctccgagtg atcgggcccg agctttatct
    12301 ggccatagct cgagaacgcg aaaaccagca cgctcaccag aatgacggca accacgagca
    12361 cgtagtacca gctcgcgccg gccacgatgg cgccctgcac atcgctgaac agggcgtcca
    12421 tggtgtcggg aaaaagtgcc gccagaaaga tcaccgcggc gagcagcgac gcggaggtca
    12481 ggaatacggg tcggtcgagc tcggcgtaag ctttgtcacc cgattgcatt cacgtcccgc
    12541 ttgcgaccaa aggggcccca ccttcttcgt tccatctcga aaattcaacc aagcgcatcg
    12601 cccaacgccg ccttcagcgg atccagccat gcctcgaagc gttgccactg atccaggcca
    12661 tcgcggtaaa tcggttggcg tacctgctcg gagctcggcg tgcggatggc acgttcggtg
    12721 cggtggaagt tcacgcaggc atcttcgaaa ggtaactcgc agaattccag tatgcgccgg
    12781 acctggccct ccaggtcgtc cacgacgtcc tcatgctgca ccctgagcac gaagccgggc
    12841 agcacttcgt cccagtgctt catcagctcg acgtagcgcc ggtagtagtg gccgatctcc
    12901 gtcagcccgt agctgaattc ctggccctcg ccgaacagct gcttgaagcc gctgaagcag
    12961 caggccatgg gatgccggcg cgcgtcgatg accctggcat tcggcagcag cagccggatc
    13021 agcccgacgt gaaaaaagtt gttgggcatc ttgtcgacga agcgcggggc gccctgccgg
    13081 tagggcaggg tttcctggag gtagtgagcg ccgagctggt ggagctgctc tggtttcagc
    13141 tcgccgagga tccgcggata gcgatccgag cggaccgagc tgcggcctcg caggcgctgg
    13201 gcaatggcga gcagcgacgg cagctccatg gtcccgtcga ccatggagtg cgaggcgagg
    13261 atctgctcta gcagcgtcga gcccgcccgc ggcagcccga cgacgaagat cgggtccggc
    13321 gcgggatctc cgtgaccctc ctggcgggcg aacagctctc tggtgcagac cgccatctgc
    13381 gcttccaccc gtcgatcgat ctggccggcg tcgtagcgaa tctcgccctg cttcaggcgg
    13441 ttgccgcgct cgtagtactc gaagctctcc tgatagctgc ccgcgtcctc gaaggccttg
    13501 cccgcggcga agcacaggtg aatacggtcc tgcgtgccgc acgtcggatc cgcctccgcc
    13561 tgccgcatcc catcgatctc gtcgggcgtg aaccgatagg tcttcgtgtt ggccaggctc
    13621 cagtaagcat cgccgaagtc gcggcgaacc ccgtaagcct gctggtagct tgcgacggcg
    13681 ccatcaatgt cgccgatggt tttctgagca tgacccagag aaacgtggag ttgcggcagg
    13741 tcaggatggc ttgcaagtat ctgccgatac tgagtgatgg cagcgtcatg atccccgctg
    13801 ccggtcgtgg ccatggcctc ggcaatgcga tagggcacgt gatcgggccg ctggagaagc
    13861 aagggacgca gcgcttccag cgccttggtg aacttctgcc tgcgaatcag aacgtttgcg
    13921 taatccgctc gagccagctc atggtcgggc tcgaactcca gcgcgctttc taagagaaac
    13981 tcggcgtcgt cgagtacctg caggcgcacg ccgatctccg ccagcagacg catggccggg
    14041 atgtgggtcg ggttctcctg caggtagtcc cggcagatct gctcagcctt gtacagacgg
    14101 tcctcgttga gatgactggc ggccgccagt atctctctgg gaagcgcgct cagctcgtcg
    14161 aggcgtctcg cggcctgctc ggcgagctcg cgttctccgg ccgctccgtg gagtttcacc
    14221 aggctgcgcc agctggcaac cagggcggag ttgtgggtga cggcgtgacg gaaatattcg
    14281 cgcgcggcgt tcgggtcgtt gcgcttgagc gcaacgtacc cccgctcctg ccaggctcgt
    14341 gcatgccggg gctccgacgc cagcagctcc tgaagcgcgg cctccgcccg ttcgcagtct
    14401 ccagcgtagc ggcacgccac ggccagcagg taacgtgccg cggaggtgtc ggcgtcgccg
    14461 cgaccgagca gtgcttccgc ctgctggacc gcggcatcga actgcccctg ctccagcgcg
    14521 cggcgagccg atgccaggct cggctcgcgg tgatcggggc ccggcccgct cgacgcgacg
    14581 ctcacggccc gcccttgccc cctagaagtg gtcgtaggag acgcgcaggc cgaagctgcg
    14641 gggccggttg gtgaccacct tgggggtgaa gtccgccgag ttgctcgtgg tgatgtgaat
    14701 ctgggcggac tcatcagcca ggttcttcac gaaaaactcc gccgtccagc cgttcttctg
    14761 cacccccacg gccaggtcta ccagcgcgta ggattcctgc ttgtagcgac cgttctcgaa
    14821 ggtctctccc gtggcgggaa tcggcgagct gtaggttccg ccttcggact tgatcttcag
    14881 cccgctgccc acgccgtaga cctcctggtg gacctgctcg gctaagaacg cgttgcctgt
    14941 gatgcccgag aggctctcgc cccggtagct cacgccgagc tgcacgtagc cgtttgcctc
    15001 gaaggcgtcc agcgggaact ggtagcgggc gcgaatgttg ccggagaagt tcggggcgaa
    15061 cggcagctca ctgccctcgg gaacggcgat gccctgcagc tggggattca gccgggtgat
    15121 ctcggtgtcg acgatgctga acgccccgga gacggtcagc cggtcggtcg cgagccaggt
    15181 gaagtcggcg tcgaagcctt tgatctccgc gtcgcccacg ttttccagga agaccaggaa
    15241 ggccacgttg ctcgggtcga agcgcgcggt ctgcaggtcg ctgatctccg agtagtaggc
    15301 cgtggcgttg aagcgcaggg tgcggtccag gaagtcgccc ttcatgccca gctcgtagtt
    15361 ctgcaactcg tcggtctcgg cgtatgccgg tatcaggaag ccctcgaacg gaccggtctg
    15421 attggctgac gcctggccgg cgttgcggtt gctggccggg ggcctgaagc cctcggagaa
    15481 cgttgaaaag agcatgatct ggtcgttcag ccgccagtcc agcgcgaacc ggaagatggt
    15541 gtcgtcgatc ttaacgaccc cgtcactgtt cagatcgtcg atgtcgaagg ttccggcagc
    15601 cagggcgtcg aggagcagct gcgggttgtt ggtaccgcga aacaacggcc gctgaccgct
    15661 cgggctgagc gcagcaccaa tcagatctgc atcgcccgtg cccaggacct ccagtcgttc
    15721 cgacacgtcg ttggagtagc cggtgccgtt gcacgacccg tcaggattga gcggcccgtc
    15781 ggggaattcg tccgtgccgt cccggaactt gcagccaaag gagaagttgc tggcgccgcc
    15841 gaagtcgaag tcgatctcgt agtagcgggc acccacggac agcgtcaccg catccgtgag
    15901 gtcgaaggcg aactcgccga agaaggccag ctgctcctgg tcgcgagtga agtcgttgac
    15961 gaaggtggtg ttcggtccga actgaacgtt gccggcgttg gctcc
//
LOCUS       DBNHLM010000005         8022 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1257612, whole genome shotgun sequence.
ACCESSION   DBNHLM010000005 DBNHLM010000000
VERSION     DBNHLM010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8022)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1257612"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="AC16QF_00165"
     CDS             <1..468
                     /locus_tag="AC16QF_00165"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR6446992.1"
                     /translation="PEVLLDGQVGEDRVVLKDVPEHQWRRIHGDCHLGNLLWRGEVPH
                     FVDFDDCVNGPPIQDLWMLLSGEHHEREAQLSMILEAYDQFASFDLSSLRLIEPLRTL
                     RIMHHAAWIGRRWSDPAFPRAFPWFNTEKYWSEHILSLREQMAALDEPTLTVS"
     gene            complement(449..1585)
                     /locus_tag="AC16QF_00170"
     CDS             complement(449..1585)
                     /locus_tag="AC16QF_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR6446993.1"
                     /translation="MLDFEAIRPYEDPEVAGVVARLVEDAELDRAVAVYLMPGLARVW
                     PRLASVLVRAYLRRKTKGLVTVNDVQLFLGKYMARLIEETVVELTVDGLDELSKGKPY
                     LFMSNHRDIVMDSGILNYVIHRAGHQTSRIAVGDNLFARPFAADLMRLNKSFVVERKV
                     SGTRAVYAALSRTSHYIRQSLEEGESVWIAQREGRSKDGFDRTDPALLKMLGLAYRKE
                     AGSFGDLVTRIRIVPVAVSYELDPCDLRKAHELYLTEREGSYIKPPDEDFQSIVEGMM
                     GFKGRVHLHFGSPVTGYFETAEALALEIDRAIVAGLKIFPTQAQAAARLGQASCHVQL
                     AELPAVTAAFEQRLAGCPDAEKPYLLAAYANVLRNRRELSLPSA"
     gene            1684..2649
                     /locus_tag="AC16QF_00175"
     CDS             1684..2649
                     /locus_tag="AC16QF_00175"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6446994.1"
                     /translation="MGLGIRPFDKGLLDLGNGAYAWLQPDGSWGWSNAGLVVDGDASL
                     LVDTLFDKHLTREMLSAMRDAAPKATRQFDTLVNTHSNGDHCNGNELVTGAEIVASKA
                     CAEELAAENPRMMQTMMERAPEMGEVGEFFVHCFGAFDFTNINHTLPTRTFEGELGLT
                     VGDKAVLLKQVGPAHTRGDVLAYVPDEKLIYTGDILFIEGHPILWVGPVGNWIEACDY
                     MLGLDLENVVPGHGPVTDKRGVMAVRDYLIYIRDEARKRFDAGMPPDEAAVDISLTDY
                     DSWGDAERIVVNVAVLYKEFAADPEPGDIAELFALMAKVHKARRR"
     gene            2646..3137
                     /locus_tag="AC16QF_00180"
     CDS             2646..3137
                     /locus_tag="AC16QF_00180"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6446995.1"
                     /translation="MTDLRRAGEDDLPALVRIYNHYVAHSHCTFDIETYSVDERRGWW
                     RRFDAARYQCWIAEHAGRVIGYACSQPLKAKGGYETSVEVSVYVDAAHTGSGTGRSLY
                     QQLLAALAPEDVHRAYALVALPNDPSIALHQTMGFREVAHLGEVGRKFGRYWDVVWLE
                     RAL"
     gene            complement(3134..3886)
                     /locus_tag="AC16QF_00185"
     CDS             complement(3134..3886)
                     /locus_tag="AC16QF_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR6446996.1"
                     /translation="MDPWTLGIFLGGALFGSYVQSVTGFAMAMILVAVASAAGNSQVS
                     VVAAVVSLLALVNVALALRGHLERVEARLFVWLAVGQLPAIWAGIYLLEVLEGQAVRA
                     LEVVLGVFIVLGSLSMMLRPQPRPSTSPPWACVAAGVTGGLAGGLFAASGPIMGWFNY
                     RQPLPVAAIRATLLCGFAMTTSVRTVLVGVNGGLTGEVWLLTGLALPVVVLGTWAGRT
                     FEPPVSELAMKRLAFAVLLVMGIWILLRAALG"
     gene            3968..4744
                     /locus_tag="AC16QF_00190"
     CDS             3968..4744
                     /locus_tag="AC16QF_00190"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6446997.1"
                     /translation="MDLGLTGKTALVSGSYRGTGLVIARHLAAEGVRVFVHGLEPGQA
                     ERAVEEIGSGDPVTGDITCDAGAREVAEALGDSRVDILVNNYGTADRGTWQDSDTGDW
                     LDAYQKNVLSAQRLIRAYFAPMRERGWGRIINLGTVGSTRPNSRMPHYYASKGALATM
                     TISLAKEASGTGVRVNLVSPGLILTPEVEAAYLERGRAKGWGETWEEVEPNVAADIPI
                     GRIVRREEVADLVTFLASPLADAIHGQNIRVDGGALDVLS"
     gene            4741..5127
                     /locus_tag="AC16QF_00195"
     CDS             4741..5127
                     /locus_tag="AC16QF_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6446998.1"
                     /translation="MTGPGLTRPGIRLLGLAALGLCVLAYDAGSYTLLHRLVLPIAMG
                     VAAGLMVQSAAAVLLAGTLLAGVHARPADPDWVIGIAYPALAALCGVGLTWIVVLRFR
                     RRIKATREARWRGRRERSAAPEGSES"
     gene            5129..5980
                     /locus_tag="AC16QF_00200"
     CDS             5129..5980
                     /locus_tag="AC16QF_00200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6446999.1"
                     /translation="MGTRCFLVSGTTSGIGLATARALAATGGTVVTANRDPDRGESTA
                     ALLRDATSNPNVINTVCNLSSLASVRSCAARIREQFPRIDVLINNAGVWTAKPQLSED
                     GFELSFATNHLGPFLLTHMLLEPLRAAGGARIVNVASVQHRAADLDLDHLTPKTPYRG
                     LRAYSRSKLANVMTTLAFARRFDAAEISANCLHPGVVASNIVPENSPLLRRAARVVSG
                     LMRSPARGAATSIYLATSPEVTGVTGKYFDPDQRIIQPSAEARDEAAQDLLWERSLEL
                     TGLAEAG"
     gene            5982..7748
                     /gene="aceK"
                     /locus_tag="AC16QF_00205"
     CDS             5982..7748
                     /gene="aceK"
                     /locus_tag="AC16QF_00205"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HIR6447000.1"
                     /translation="MTRGKSAMASAADGIARAIAMGYESYQAEFQAITAMAQSRFEGQ
                     DWAGVQDAGARRLAIYGEHVRDVVAEVHTLTGGSEIDASLWRKSKQAFTSIISEHYNA
                     ELYETFYNSIHREMTDDSVVDDTEMFVRTLFPIPPVQPAEPVFERHAIAGEPEEFVRT
                     LLDGLAINRPWVDRDRDVRNILRSLSEERPEIQDPLRLDVDILKPIFYRNKGAYVVGR
                     LLYKDEGGAQLWPFALALLLDDDGRLYVDTLICDVDELSVMFSFTRAYFMVLTDYPHA
                     LVDFLQVLLPNKKRTELYASMGLHKHGKTVFYRDFLDHLSRTDDQFVIAPGTPGMVMT
                     VFTLPSLQTVFKVIKDEFAPQKNITAQQVRDKYYVVKSHDRVGRMADTQEFQNLTFPI
                     DRFDPELVEELRRVAPSSIEISGNEILIRHLYTERLMTPLNLFIDTADEGALREALEE
                     YGNAIKQLAAANIFPGDMLLKNFGVTRHGRVVFYDYDEICYLTDVNFRDIPEPRSPED
                     EMAAEPWYAVGPHDVFPEEFRRFLFGRRKIKQMFEEMHGELFEPSYWRGLQAAINDGQ
                     VMDVFPYRRKRRFGEHGAASRA"
     gene            7765..>8022
                     /locus_tag="AC16QF_00210"
     CDS             7765..>8022
                     /locus_tag="AC16QF_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447001.1"
                     /translation="MEQLDRRARERLEKADALIFDFDGTLAPNLDLPDMRRQVVDLTL
                     GRGVPGEIFEHLYIVEIIEAAASWLRRGDRTSAADYHEAAHR"
BASE COUNT         1488 a         2673 c         2566 g         1295 t
ORIGIN      
        1 ccagaggttc tcctcgacgg tcaggtcggg gaagaccgag ttgtcttgaa ggatgtaccc
       61 gagcaccaat ggcgccgcat ccacggcgac tgccacctgg gcaacctgct gtggcgcggc
      121 gaggttccac acttcgtcga tttcgacgac tgcgtgaacg gtccgccgat acaggacctg
      181 tggatgctgc tgtccggcga gcaccacgag cgcgaagccc agctgagcat gatcctggag
      241 gcttacgatc agtttgcgag cttcgacctg tcgagcctca ggctgatcga acccctgcgc
      301 acgctgcgaa tcatgcacca cgctgcctgg atcggccgcc gctggagcga tccggccttt
      361 ccccgtgcgt tcccgtggtt caacacggag aagtactggt cggaacacat tctcagcctg
      421 cgagagcaga tggccgctct cgacgagcct acgctgacgg taagctgagc tcccggcgat
      481 tgcgcaggac gttggcgtac gccgccagga gatagggctt ctcggcgtcg gggcacccag
      541 caagacgctg ctcgaaggcg gcggtcacgg caggaagctc ggccagctgc acatggcagc
      601 tggcctggcc gagccgggcc gccgcctgcg cctgggtggg aaagatcttc aggcccgcga
      661 cgatggcgcg atcgatctcg agggcgaggg cttccgcagt ttcgaagtag ccggtcacgg
      721 gcgagccgaa gtgcagatgc acgcgaccct tgaagcccat catgccctcc acgatgctct
      781 ggaaatcctc gtccggcggc ttgatgtagg agccttccct ttccgtcagg taaagctcgt
      841 gggctttgcg cagatcgcag ggatcgagct cgtaggagac ggctaccggg acgatgcgga
      901 ttcgcgtgac gagatccccg aagctccccg cttccttgcg gtaggcgaga cccagcatct
      961 tcagcagggc cggatcggtg cgatcgaaac cgtccttcga tcgaccctcg cgttgcgcga
     1021 tccacaccga ctcgccctcc tcgagcgact ggcggatgta gtgggacgtt cgcgaaagcg
     1081 cggcgtagac ggctcgggta ccgctgactt tgcgctcgac cacgaagctt ttgttgaggc
     1141 gcatcaggtc ggcggcgaac ggccgcgcga acaggttgtc cccgaccgcg atgcgcgacg
     1201 tctggtgccc cgctcgatga atcacgtagt tgaggatgcc cgagtccatc acgatgtccc
     1261 gatgattcga catgaacagg tagggcttgc ccttagacag ctcgtccagg ccgtccaccg
     1321 tcagctccac gacggtctcc tcgatcaggc gggccatgta ctttcccagg aacagctgaa
     1381 cgtcgttgac ggtgaccagc cccttcgtct tgcgtctgag gtaggctctg accaggacgc
     1441 tcgccaggcg gggccagacc cgggccaacc cgggcatcag gtagacggcc accgcgcggt
     1501 cgagttctgc gtcttcgacc aggcgcgcga ccacccccgc aacctccggg tcctcgtagg
     1561 gtcgaatggc ttcgaagtcc agcatgtgcg ccagttagcg ccatttgaca ggcagaaaac
     1621 aagtcgccgc ccgggcggct tttcctctaa catgaggagc ctgctcggtg ggggagacat
     1681 ctgatgggcc taggcattcg cccctttgac aagggcctgc tcgacctggg aaacggcgca
     1741 tacgcctggc tccagcccga cggcagctgg ggttggtcaa acgccggcct ggtggtcgac
     1801 ggggacgcct ccctgctggt ggacacgctc ttcgacaagc acctgacccg cgagatgctc
     1861 agcgccatgc gcgacgccgc acccaaggcc acccggcaat tcgacactct ggtcaacacg
     1921 cactccaacg gcgaccactg caacggtaac gagctggtga ccggggccga gatcgtcgcc
     1981 tcgaaagcct gcgcggaaga gctcgccgcc gagaatccaa gaatgatgca gacgatgatg
     2041 gagcgcgcgc cggaaatggg cgaagtgggc gagttcttcg ttcactgttt cggcgctttc
     2101 gacttcacca acatcaacca cactctgccc acccgtacct tcgagggaga gcttggactg
     2161 accgtcggtg acaaggcggt gctgctcaag caggtcgggc ccgcccacac ccgaggcgac
     2221 gttctggcct acgttcccga tgagaagctc atctacacag gcgacatcct cttcatcgaa
     2281 ggccacccga ttctctgggt gggccccgtg ggcaactgga tcgaagcctg cgactacatg
     2341 ctcggtctgg acctggagaa cgtggtgccc ggacacggtc ccgtcacgga caaacgcggc
     2401 gtgatggccg tgcgcgacta cctgatctac attcgtgacg aggctcgcaa acgattcgat
     2461 gccggcatgc cgccggacga ggcggctgtg gacatctcgc tgacggacta cgactcctgg
     2521 ggcgacgccg agcggatcgt ggtcaacgtg gccgtgctct acaaggagtt cgctgcagat
     2581 cccgagcccg gcgacatcgc cgagctgttc gcgctgatgg ccaaggtgca caaggcccgg
     2641 cgccgttgac cgacctgcgc cgcgccgggg aggacgatct gcctgccctg gtccggatct
     2701 acaaccacta cgtggcccac tcccactgca cgttcgacat cgaaacctac agcgtcgacg
     2761 agcggcgagg gtggtggcgg cggttcgacg ccgcccgata ccagtgctgg atcgccgagc
     2821 acgccggcag ggtcatcggc tatgcctgca gccagccgct gaaagccaag ggcggttacg
     2881 aaacctcggt tgaggtgagc gtctacgtgg acgccgccca tacgggctca ggaaccggac
     2941 gctccctcta ccagcagctg ctcgcagcgc tcgcgcccga ggacgtgcac agggcctacg
     3001 ccctggtcgc cctgcccaac gatccctcca tcgctttgca tcaaacgatg ggcttccggg
     3061 aggtggcgca cctcggcgag gtcggccgga agttcggccg ctactgggac gtggtctggc
     3121 tggaacgcgc cctttagccg agcgcggcgc gcagcaggat ccagataccc atgacgagga
     3181 gcacggcgaa cgccagccgc ttcatggcga gctcagatac cgggggctcg aaggtgcggc
     3241 ccgcccaggt tcccagcacc accacgggca aagccagccc ggtgagcagc cacacctccc
     3301 ccgtcaaccc gccattgacc cccaccagca ccgtgcggac cgacgtcgtc atggcgaagc
     3361 cgcacagcag cgtggcccga atggccgcca cgggcagcgg ttgccggtag ttgaaccatc
     3421 ccatgatcgg acccgacgcc gcgaacaggc caccggccag gccgccggtc acgcctgcgg
     3481 ccacgcaggc ccacgggggt gacgtggagg gcctcggctg cgggcgcagc atcatggaaa
     3541 ggctgccgag cacgatgaag acgccgagca cgacctccag cgcccgcacg gcctggccct
     3601 ccagcacttc cagaagatag atgccagccc atatggccgg cagctgaccg accgcgagcc
     3661 agacgaaaag acgcgcctcc acccgctcga gatgaccgcg cagcgcgagc gccacgttca
     3721 cgagagcgag caggctgacc accgccgcca ccaccgagac ctgagaattg ccggcggcgc
     3781 tggccacggc caccaggatc atggccatgg cgaagccggt cacggactgc acgtagctgc
     3841 cgaacaacgc gccgccaagg aagatgccga gagtccaggg gtccatcgcg gacggcagta
     3901 taggcgatcg cggcagatcg ggtggtatag tcccggcctc catcgggggc acgacggaaa
     3961 agcaggaatg gatctcggcc tcacaggcaa gacggcgctc gtcagcggca gctatcgggg
     4021 gaccgggctc gtcatcgccc gccatctggc agccgagggc gtgcgggtat tcgtccacgg
     4081 gctggagccc ggtcaggcgg agcgcgccgt ggaggaaatc ggcagcggcg acccggtgac
     4141 cggcgacatc acctgcgatg ctggcgcccg agaggtggcc gaggccctgg gcgacagccg
     4201 cgtcgacata cttgtcaaca actacggaac cgcggaccgg ggtacctggc aggatagcga
     4261 caccggcgac tggctggacg cctaccagaa aaacgtgctc tccgctcagc gtctgatccg
     4321 agcgtacttc gctccaatgc gggagcgggg ctggggaagg atcatcaacc tcgggaccgt
     4381 gggatccacc cgacccaaca gccggatgcc ccattactac gcgtcaaagg gcgcgttggc
     4441 caccatgacc atcagccttg ccaaggaggc atcgggcacg ggagtgcggg tgaatctggt
     4501 ttctcccggc ctgatcctca cccccgaggt ggaggccgcc tacctcgagc gcggccgagc
     4561 caagggctgg ggagaaacct gggaagaggt ggagccaaac gtggcggccg acattcccat
     4621 cgggcgcatc gtccgacgtg aggaggtggc cgacctcgtc acgttcctgg caagcccact
     4681 ggccgacgcc attcacggcc agaacatccg ggtggacggc ggggcgttgg acgtgctgtc
     4741 ttgaccggac caggcctgac cagaccgggt atccggctgc tcgggctggc cgcgctgggc
     4801 ctgtgtgtgc tcgcctacga cgcgggcagc tacaccctcc ttcaccgtct cgtcctgccc
     4861 atcgccatgg gcgtggctgc aggcctgatg gtgcagagtg cggccgccgt tctgctcgcc
     4921 ggcaccctgc tcgccggcgt tcacgcccgt cctgcggacc cggactgggt catagggatc
     4981 gcctatccgg cgctggccgc tctgtgtggc gtggggctga cctggattgt cgtgctgcgt
     5041 ttccgccggc ggatcaaagc cacgcgcgag gcacgctggc gcggccgtcg cgagcgctcg
     5101 gcagcgccgg aaggatcgga gagctgaagt gggaacacgc tgcttcctgg tcagcggcac
     5161 caccagcggc atcggtctgg ccaccgcacg cgccctcgcg gccaccggag gcaccgtcgt
     5221 cacggcaaac cgcgaccctg acaggggcga gagcacggcc gccctgctgc gggatgccac
     5281 cagcaacccc aacgtcatca acaccgtgtg caacctgtct tcgttggcat ctgtccgctc
     5341 ctgcgcagcc cgcatccggg agcagttccc gcgcatcgac gtgctgatca acaacgctgg
     5401 cgtgtggacc gccaagcctc agctttccga ggacggcttc gagctttctt tcgccacgaa
     5461 tcacctcggg ccgtttctgc tcacccacat gctcctcgag ccgctgagag cagccggcgg
     5521 cgcaaggatc gtgaacgtcg cctcggtgca gcaccgcgcg gcggacctgg acctggatca
     5581 tctgacccca aagacgccct atcggggtct gcgggcctac agccggtcaa agctcgccaa
     5641 cgtgatgacg acgctcgcct ttgccaggcg cttcgacgcc gcggagatct ccgcaaactg
     5701 cctccatcct ggcgtggttg cctccaacat cgttcccgaa aacagccccc tgctgcgcag
     5761 agctgcgcgt gtcgtgagcg gcctgatgcg cagccccgcc cgcggggccg caacttccat
     5821 ctacctggca acgtcgccag aggtgacagg ggtgacgggc aaatacttcg accccgacca
     5881 acgcatcatc caaccgtccg cggaagcccg ggacgaggcg gcacaggacc tgctctggga
     5941 acgcagcctc gagctgaccg ggctggcgga ggccggctga aatgacacga ggcaaatccg
     6001 ccatggcgtc cgcggccgac ggcatcgccc gagcgattgc catggggtac gaatcctatc
     6061 aggcggagtt tcaggccatc accgccatgg cccaaagccg tttcgaaggc caggactggg
     6121 ctggcgttca ggacgcgggg gcgcgtcgcc tggccatcta cggggagcac gtgcgcgacg
     6181 tggtcgctga ggtccatacg ctcacgggag ggtccgagat cgacgcttcg ctgtggcgca
     6241 aatccaagca ggcgttcaca tcgatcatca gcgagcacta caacgccgag ctctacgaga
     6301 cgttctacaa ctccattcat cgcgagatga ccgacgacag cgtagtcgac gacacggaga
     6361 tgttcgttcg taccctgttc cccatcccgc cggtgcagcc agccgagccg gtcttcgaaa
     6421 gacacgccat cgccggcgaa cccgaagagt tcgtgcgcac gctgctcgac ggtctggcca
     6481 tcaaccggcc atgggtcgac cgggaccgcg acgtgcggaa catactgcgc tctctttccg
     6541 aggagcgccc ggagatccag gacccgctca ggctggacgt ggacatcctg aaacccatct
     6601 tctacagaaa caagggcgca tacgtggttg gccggctgct ctacaaggac gagggcggag
     6661 cacaactgtg gccatttgcg ctcgccctgc tcctggacga cgacgggcgc ctctacgtgg
     6721 acacgcttat ctgcgatgtg gacgagctgt ccgtcatgtt cagcttcacc cgcgcctact
     6781 tcatggtcct gacggactac cctcacgcgc tggtggactt cctgcaggtc cttctgccca
     6841 acaagaagcg caccgagctc tatgcctcca tgggacttca caagcacggt aaaacggtct
     6901 tctatcgtga ctttctggat catctgagcc gcacggacga tcagttcgtg atcgcgcccg
     6961 gaacgccggg catggtgatg accgtgttca cgttgccctc cctgcagacc gtgttcaagg
     7021 tgatcaagga cgagttcgcg ccccagaaga acatcaccgc ccagcaggtc cgggacaagt
     7081 actacgtggt gaagtcccac gatcgggtcg gccgcatggc cgatacccag gagtttcaga
     7141 acctcacctt ccccatcgac cggttcgatc cggagctggt ggaggaactc aggcgcgtag
     7201 caccctccag catcgagatt tccggaaacg agattctgat ccgccacctg tacacagagc
     7261 ggctgatgac ccctttgaac ctgttcatcg acacggccga tgaaggggcc ctgcgcgaag
     7321 cgctggaaga gtacggcaac gccatcaagc agctggctgc tgccaacatc tttcccggcg
     7381 acatgctgct caagaacttc ggcgtcaccc ggcacggccg cgtggtgttc tacgactacg
     7441 acgagatctg ctacctcacc gacgtcaatt tccgggacat tcccgagcca cgctcgcccg
     7501 aagacgagat ggcggccgag ccatggtatg cagtggggcc ccacgacgtg tttcccgagg
     7561 agtttcgccg tttcctgttc ggccgacgga agatcaagca gatgttcgag gaaatgcacg
     7621 gcgagctgtt cgagccttcc tactggcgcg gcctgcaggc agccatcaac gacggtcagg
     7681 tgatggacgt ctttccgtat cggcgcaaga ggcgtttcgg cgagcacggc gccgcgagcc
     7741 gggcctgaca cccaggggag accggtggag cagctggaca ggagggcccg cgagcggctg
     7801 gagaaggccg acgccctgat tttcgacttc gacggcaccc tggctcccaa cctggatctt
     7861 cccgacatgc gccggcaggt ggtggacctg accctcggac gcggcgtgcc tggggagatc
     7921 ttcgagcatc tctacatcgt ggagatcatc gaggcggccg cgtcgtggct gcggcgaggc
     7981 gatcgcactt cggccgccga ctatcacgaa gccgcccatc gg
//
LOCUS       DBNHLM010000006         9528 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_659300, whole genome shotgun sequence.
ACCESSION   DBNHLM010000006 DBNHLM010000000
VERSION     DBNHLM010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9528)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9528)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9528
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_659300"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="AC16QF_00215"
     CDS             complement(<1..1095)
                     /locus_tag="AC16QF_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR6447002.1"
                     /translation="MSSGVDLSAGPVSRHLVNLTIPMFLGISSMILASMIDTVYVGWI
                     GTRELAAVSFSFPVVLAMATVSMGIGVGAASIIARVVGAGDMERVRRLSTNALYLVAM
                     LVLSLTLLGMLFVEPLFRVLGAGSDILPLSVRYMTVWFLGLPAFALPMVATNIMRAVG
                     SARLPGVIMAAGAGLQVLIAPPLIFGIPGVADGLGITGAAWSFVLSRLAMFGYTVRVM
                     QRMNLLSFAREPWSSRRESWGEVLKIGLPSMVGNLIGPVSMGVVVALLADYGHEVVGG
                     FGVASRIESLATMIIMALAASTGPVIGQNWGARRYERIYRAQSLGFRFSFLWSLLAFA
                     LLAGFGRPIVGFINQDPAVVDATYLYLLLLP"
     gene            complement(1113..3467)
                     /locus_tag="AC16QF_00220"
     CDS             complement(1113..3467)
                     /locus_tag="AC16QF_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like protein"
                     /protein_id="HIR6447003.1"
                     /translation="MSKKELRLAVVIYGGASLAVYMHGVTKEMLKLVRASKVFHQMGI
                     GRARYASYAEGPDNRQADTEAVYFEILKEINGRSHFRVVLDVIAGASAGAINSVMLAK
                     AIVDDASLDSQTGTWLSDADVEYMGRETSPWQKWWLYPILRLLFFWLPRDVGASPETR
                     EKLARVVRSSWFQPPLSGARLSHIFFNSLEAMLKTRLPGSSLLPAGQRLDVYTSITDL
                     VGYPRSIRLHDELMASEREHGAYCRFTHMETEAGRRLSDLDDANLPGLVWAARASSSY
                     AGAFEPFHHSELKRVLAERHLSWPLEEKFLHYNVFVRDGTPAARLFDPADRYFVDGGI
                     VNNKPFEVALEALGHRPADRLVERCIVYIEPDPSAVDVVSPERNLGYLRTIRAALSSI
                     PRNQPIVDDLQAIVSQDKRVRINRRIVESNAAEINTLVRELQDVARRQEMTADLITYL
                     RTAILERAEEDMSIAYHAYVQRRVWRLTDALVDEWAVLAENPNDERTRDGMSESISRS
                     WQGAESGDAGRRDNMQETFLDRFDVTYRIRRLQFVIRRINQHEDVTELDEASTDALDS
                     FKNDAYGFLERLHRLRRSQYLDGPLIEKLAVAARNLPLEPDEARDLLKDIFGALGLQS
                     FDREFDALICTFLDRLTDDRLRDQMLTDYVGFPVFDVLLMSPASLEGGPDPLTPISVE
                     RISPEDAPTLRSVFKGLKSRQFMGFLGFFNRAYREHDYLWGRLNAADRLVDLLVQSSR
                     DAISDPDRMRLKLFRAVVDRERQQLYRCDDELDRIDAFLKAAGV"
     gene            3513..4202
                     /locus_tag="AC16QF_00225"
     CDS             3513..4202
                     /locus_tag="AC16QF_00225"
                     /inference="COORDINATES: protein motif:HMM:NF016300.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HIR6447004.1"
                     /translation="MSRIQIIALLCCAMVLAAPTIGRPQVEGIHVSGRGEVLVTPDMA
                     RVTLRVTRDGQDAVELKKELDDVTRKVLSLTRSLKIEGEDVTAAVVNIQPRYRRGGGQ
                     SVIDGVTASRTIAVVLRDLDRYGQLMNGALEVGVNSVSGTQLDSSDRAAEEEQALERA
                     MQDARAAAARVADGFGVSVGRLLDVHVGQPPVQPMRGVMMEAAAAGDDFSPGQLVISR
                     QLQATFAIETR"
     gene            4297..5418
                     /locus_tag="AC16QF_00230"
     CDS             4297..5418
                     /locus_tag="AC16QF_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6447005.1"
                     /translation="MNHRNIVAAIGCLALVACGEAPIDDAAPTARPVKTMIVESPAGS
                     GVRNFPGRIDSANRADLAFRVPGTVARLAASEGAPVNRGQELARLDQTDYEIALRDRQ
                     ATWDRASKDYERSKDLVDKGAISRRDFDRVEANFKSADAALAQAKQNLEYTTLRAPFD
                     GNVARRHVDAFEEINAGQTVYSVIDQSVLEVKIDIPENVIMMLSSRGSEPEEQAGKIP
                     VWAAFDMAPERRFPLTVKEYATRADAQTQTFEVTFALPAPDDVRVLPGMTASVTMDLS
                     RAMDVDSVYYLPVTAVTGSEGLTPRVWAVDEDTMTVREQPIVLGRLVGSSVEVTEGLE
                     PGMRIVTAGAAYLADGMAVTLLAHSEQAEPREEDLPRPS"
     gene            5452..8499
                     /locus_tag="AC16QF_00235"
     CDS             5452..8499
                     /locus_tag="AC16QF_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6447006.1"
                     /translation="MNIGSYSVENKVISWLLVIILVAGGIYGFNEMGKLEDPNFTIKQ
                     AKIITLYPGATAQEVQDEVTYHIEEALQLMGQLKRIKMSISRPGMSDLAIEFKDEYKA
                     DDFPDIYDELRRKLQDMHGKLPPGVQGPRVIDDFGDVYGIYAALTGDGYTYRDLKDAA
                     DELKKQLVLVPGVRKIVIGGGQREVVYIEISRTRISELGIPLEQIANILQSQNMVTDA
                     GRVRVGPDYVRIHPTGEFTSVQAIGDVLIGSNERRLIFLKDIATIHRAYEEVANKLIF
                     FNGKPALTLGVSIQAGENVVAVGEAVRKRFGQLASTIPIGMDIKVIYNQPEEVQKSVS
                     GFIVSVGQAIAIVIAVLLVFMGLRTGIIIGAVLLITVSGTLLFMQLYGIELQRISLGA
                     LVIALGMLVDNAIVVAEGMLVRIQAGMNPTKAAEETVGKTMWPLLGGTIIGILAFSAI
                     GLSTDNTGEFASSLFYVILISLTLSWVTAVSTTPLLCAMFLKPDPDADPDANPYDKGF
                     FRTFRGALKVVIRLRVLAVAVVVGLFVLAGFGFGYIKNAFFPNSNTPMFFVDIWEPEG
                     TDIRVTRRDAQAISEFLRAQEGVRSTVALIGGGDARFALTYEPKESSPAYAQIIVQTE
                     TLEQISKIWPAVEAHLQEHYPHLDPIIKAFRIGPGRDGKIEARFHGPDPAVLRSLAER
                     AKAIMREDPGAKEINDDWRQPVMLVRPVFNERVARQLGVTRENVATALKSAYEGTDVG
                     VYRDGIRMLPIKLRPPAEERADVANIQDIRVWSPALQTAVPIAQVVNSFETVFENTIL
                     RGRNRIQTIIAACNPTGELATPLFERLKPRIEDIELPPGYELSWGGEYEDSANAQAGL
                     ARVLPAGFILMILTSILLFGKVRQPLIIWLTVPLAIIGITAGLLGFDGAFDFMSLLGA
                     LSLIGLLIKNAIVLIEEIDQQIADGKEHLTAILDSTVSRLRPVVMAASTTILGLIPLL
                     QDVFFVNMSITIMAGLGFATFLTLLFVPVLYSLFFKVAYREDA"
     gene            8516..9118
                     /locus_tag="AC16QF_00240"
     CDS             8516..9118
                     /locus_tag="AC16QF_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447007.1"
                     /translation="MRAILVTVGLLLVAGCATQTTVVRADFTQGEGAYVVGYSGDPVI
                     RTRMEEQLVNDLRAREMIAFASHTDIPDITASSRYELIAAARKRGVISVIVINQVSGD
                     ASDSVVQNPERISPTHPDLQAFYDYSLGRQPVVEPGQQVFAEVNLFVLDGDEASLFWS
                     GTTWSFQADGQGTAIRGISETIADQLQKVRDQARANPFTQ"
     gene            complement(9141..>9528)
                     /locus_tag="AC16QF_00245"
     CDS             complement(9141..>9528)
                     /locus_tag="AC16QF_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447008.1"
                     /translation="ALSGVPAAAAQFDGKQDLLCSAFRMFECDMANGCTAVTPAELGA
                     SSSWIVNAKKKTITGTNPNAQPNDIDRMELLDGKLFMSGIQDGVPEERDGVAWSVSIN
                     NPDGVMTIMVAGEGLGVVGLGSCVPR"
BASE COUNT         1801 a         3105 c         3060 g         1562 t
ORIGIN      
        1 tggaagcaac aacaggtaga ggtaggttgc atcgacgacg gccgggtcct ggttgatgaa
       61 gcccacgatg ggccggccga aaccggcgag gagggcgaaa gccagcagtg accacaggaa
      121 gctgaagcgg aaccccaggg attgcgcccg gtagatgcgc tcgtagcggc gagcacccca
      181 gttctggcca atcaccggtc ccgtgctggc agccagggcc atgatgatca tggtagccag
      241 ggactcgatg cgcgaggcga cgccaaaccc gcccaccacc tcgtgcccgt agtcggccag
      301 cagggccacc accacgccca tggagaccgg gccgatgagg ttgccgacca tggacggcag
      361 gccgatctta agaacttccc cccaggactc gcgacgcgaa gaccagggct cacgggcgaa
      421 cgacaacagg ttcatgcgct gcatcaccct gaccgtgtag ccgaacatgg cgagccgaga
      481 cagcacgaat gaccaagccg ccccggtgat tcccagcccg tccgccacgc cggggatacc
      541 gaagatcagc ggcggcgcga tgagcacctg aaggccggca cccgcggcca tgatgacgcc
      601 cggcaggcgc gcgctgccca ccgcgcgcat gatgttggtg gcaaccatgg gcagcgcaaa
      661 agccggcagg ccgagaaacc acaccgtcat gtagcgcacc gacagaggca ggatgtcgga
      721 ccctgcgccc agcaccctga agagcggctc gacgaaaagc atcccgagca gggtgaggct
      781 caataccagc atggcgacca ggtagagcgc gttcgtcgac aggcgccgca cccgttccat
      841 gtcgcccgcc cccaccaccc gggcgatgat ggacgccgcc ccgaccccga ttcccatgga
      901 gaccgtcgcc atggccagga ccacgggaaa ggagaagctc accgccgcca gctcccgggt
      961 gccgatccag ccgacgtaga ccgtatcgat catcgacgcg aggatcatcg acgagatccc
     1021 gaggaacatg gggatcgtca ggttgaccag gtggcgggat acggggcccg cggacaggtc
     1081 aacgcccgag ctcacgaatc cggtctcatc cgctagacgc ccgcggcttt cagaaacgca
     1141 tcaatcctgt cgagctcgtc atcgcaccgg tacagctgct gacgctcgcg gtcgacgacg
     1201 gcgcgaaaga gtttgaggcg catccgatcg gggtcgctga tggcatcacg agacgactga
     1261 acgagcagat cgaccagccg gtccgcggcg ttgagcctgc cccagaggta atcgtgctcg
     1321 cgataggccc ggttgaagaa accgagaaaa cccatgaact ggcgcgactt cagtcctttg
     1381 aagaccgacc tgagcgtcgg cgcgtcctcc gggctgatcc tctccacgct gatgggcgtg
     1441 agcgggtcgg ggccgccctc cagggacgcc ggcgacatga gcagcacgtc gaaaaccgga
     1501 aagccgacgt aatccgtgag catctggtcg cgcagccggt cgtcggtaag ccggtcgagg
     1561 aaggtgcaga tcagggcatc gaactcgcgg tcgaacgact gcagccccag cgcgccgaat
     1621 atgtccttga gtagatccct ggcctcgtcc ggctcgagcg gcaggttccg ggcggccacc
     1681 gcgagctttt cgatcagcgg gccgtcgagg tattgggatc gccgcagtcg atgcaggcgc
     1741 tcgaggaacc cgtaggcgtc gttcttgaag ctatccagcg cgtcggtgga cgcttcatcc
     1801 agctccgtga cgtcctcgtg ctgattgatc cgccggatga cgaactgcag gcgccggatg
     1861 cggtaggtga cgtcgaatcg gtcgaggaac gtttcctgca tgttgtcgcg ccggccggca
     1921 tccccggact ctgccccctg ccacgagcgc gatatggact cgctcatgcc atcccgcgtt
     1981 cgctcgtcgt tggggttctc ggcaagcacc gcccactcgt cgacgagcgc atcggtcagc
     2041 cgccagacgc gccgctgcac gtaggcgtga taggcaatgc tcatgtcctc ctcggcgcgc
     2101 tccaaaatag ccgtccgcag gtaggtgatc agatctgccg tcatctcctg gcgcctggcc
     2161 acgtcctgca gctcgcggac cagggtattg atctcggccg cattggattc gacgatgcgc
     2221 cggttgatgc gcacccgctt gtcctgggac acgatggcct ggagatcgtc tacgatgggc
     2281 tggttgcggg gaatcgagga gagcgccgcg cggatcgtgc ggaggtatcc caggttacgc
     2341 tccgggctca cgacgtccac ggcgctcgga tcgggctcga tgtagacgat gcagcgttcg
     2401 acgagccgat cggccggacg gtgaccgagg gcttccaggg ccacctcgaa aggcttgttg
     2461 ttgacgatgc cgccgtccac gaagtatcgg tccgccgggt cgaaaagacg cgccgccggc
     2521 gtgccgtccc ggacgaacac gttgtagtgc aggaatttct cctccagcgg ccagctcaag
     2581 tgccgctcgg cgagcacgcg cttgagctcg gagtgatgaa acggctcgaa agcccccgca
     2641 taggacgagc tggcccgggc cgcccagact aggcccggca ggttggcgtc gtccaagtcg
     2701 ctgagccggc ggccggcctc ggtttccatg tgggtgaatc ggcagtaggc accgtgctcc
     2761 cgctcactgg ccatcagctc gtcgtgaagg cggatgctgc ggggatagcc gaccaggtcc
     2821 gtgatgctcg tgtacacgtc caaacgctga ccggcgggca gcaggctgct ccctgggagc
     2881 cgggtcttga gcatggcctc cagagagttg aagaagatgt ggctcaaccg ggcccccgac
     2941 aacggcggct ggaaccagga ggagcgcacg acgcgggcga gcttctcgcg ggtttccggg
     3001 ctggcaccga cgtccctcgg aagccagaag aacaggagcc ggagaatcgg gtaaagccac
     3061 cacttctgcc agggcgacgt ctctctgccc atgtactcca cgtccgcatc ggacagccag
     3121 gttcccgtct gggaatcaag cgacgcatca tcgacgatgg ccttggccag catcaccgag
     3181 ttgatggcgc ccgccgacgc gccggcgatg acgtccagaa ccacccggaa gtgactgcgc
     3241 ccatttatct ccttgagaat ctcgaaatag acggcctcgg tatctgcctg gcggttgtcg
     3301 ggaccctcgg catacgaagc gtatcgggca cggccgatgc ccatctggtg aaaaaccttg
     3361 gaggcgcgga cgagcttgag catctccttg gtgaccccat gcatgtagac ggcgagggaa
     3421 gccccgccat atatcaccac cgcgagccgt agttctttct tcgacatggt tagccattat
     3481 agtggcgcga actgcccaac ggagtacgac ccatgtcccg aatccagatc atcgccctgc
     3541 tgtgctgcgc aatggtgctc gccgccccca ccatcggccg tccccaggtg gaaggaattc
     3601 atgtgagcgg tcggggcgag gtgctcgtga ccccggacat ggcccgggtc acccttcgcg
     3661 tcacccgcga cgggcaggac gccgtcgagc tcaaaaaaga gctggacgac gtgacacgca
     3721 aggtgctgtc cctgacgcgc tctctgaaga tcgagggtga ggacgtgacc gcggcggtgg
     3781 tcaacatcca acctcgctac cgccgtgggg gcggccagag cgtcatcgac ggggtcaccg
     3841 catcccgcac catcgcggtg gtattgcgcg atctcgaccg ttacggccag ctcatgaacg
     3901 gcgctctgga agtgggggtg aactcggtaa gcggtaccca gctcgactcc agcgaccggg
     3961 cggcagagga agagcaagcc ctggagcggg cgatgcagga cgcgcgcgcc gcggctgctc
     4021 gcgtcgccga cggttttggc gtctccgtgg gcaggctgct ggatgtgcac gtgggccagc
     4081 ctcccgtcca gcccatgcgc ggcgtgatga tggaggcggc ggcagccgga gacgacttca
     4141 gcccgggaca gctggtcatc tcccgccagc tgcaggccac attcgccatc gaaacccgtt
     4201 gacttctgcg cggcggccaa agactccgta tggacctctc tggagaacgg ctccgaatgc
     4261 gggcagtata gccccgggaa agtggaggac cggatcttga accacaggaa tatagtggcc
     4321 gccatcggct gcctggccct ggtcgcgtgc ggcgaagcgc ccatcgacga cgcggccccc
     4381 acagcgcggc cggtgaagac gatgatcgtc gagtctcccg ccggcagcgg cgttcgcaac
     4441 ttccccggcc gaatcgattc ggcgaacagg gcggacctgg cctttcgcgt accgggcacg
     4501 gtagccaggc tcgcggcctc ggaaggcgcg cccgtcaatc ggggacagga gctcgcgcgc
     4561 ctggaccaga ccgactacga gatcgcgctg cgtgaccgcc aggccacctg ggaccgggcc
     4621 agcaaggact acgagcgctc caaggatctg gtggacaaag gggccatctc ccggcgcgac
     4681 ttcgaccggg tggaggccaa cttcaagagc gccgatgcgg cgctggccca ggcaaagcag
     4741 aacctcgagt acaccaccct gcgcgcaccc ttcgacggca acgtggccag acggcatgtg
     4801 gatgccttcg aggagatcaa cgccggccag accgtctact ccgtgatcga ccaatccgtg
     4861 ctggaagtga aaatcgacat cccggaaaac gtcatcatga tgctgtcatc gcgcgggtcc
     4921 gagcccgagg aacaggccgg caaaatcccg gtatgggccg ccttcgacat ggccccggag
     4981 cgccgctttc ctctgacggt aaaggagtac gccacacgcg ccgacgctca gacacagacc
     5041 ttcgaggtga cgttcgccct gccggcacct gacgacgtca gggttctgcc cgggatgacg
     5101 gccagcgtca ccatggatct gtcccgggcc atggacgtgg actccgtcta ctaccttccc
     5161 gtcacggccg taaccggcag cgagggcttg accccaagag tctgggccgt ggatgaagac
     5221 accatgaccg tgcgcgagca gccgatcgtg ctggggcgcc tggtggggtc cagcgtcgag
     5281 gtcaccgagg gactggagcc cggcatgcgc atcgttaccg ccggagccgc ctacctggcg
     5341 gatggcatgg cggtaacgct gctggcccac agcgagcagg ccgagccgcg ggaagaagac
     5401 ctgccgcgcc cgagctgacg gctcgcgcgg cgctaacgga caacaacaac gatgaacatc
     5461 ggctcatact ccgtcgagaa caaggtcatc tcctggctgc tggtcatcat tctggtggcc
     5521 ggcggaatct acggcttcaa cgagatgggg aagctggagg atccgaactt caccatcaag
     5581 caggcgaaga tcatcaccct gtatcccggg gctacggctc aggaagtgca ggacgaggtg
     5641 acctaccaca tcgaggaagc gctgcagctt atgggccagc tcaagcgcat caagatgtcc
     5701 atctcccgac cgggcatgtc cgatctcgcc atcgaattca aggacgagta caaggccgac
     5761 gacttcccgg acatttacga cgagctgcgc cgcaagctgc aggacatgca cggcaagctg
     5821 ccgcccggcg tgcaggggcc acgcgtcatc gacgacttcg gcgacgtcta cggcatctac
     5881 gcggcgctga ccggcgacgg ctatacgtat cgtgacctga aggacgccgc cgacgagctc
     5941 aagaagcagc tcgtgctggt acccggcgtg aggaagatcg tcatcggcgg cggccagcgc
     6001 gaggtggtct acatcgagat ctcacgaacg cggatcagcg agctcggcat accgctggag
     6061 cagatcgcca acatcctgca gtcccagaac atggtcacgg acgcgggacg ggttcgggtc
     6121 ggacccgact acgtccgcat tcatcccacc ggcgagttca cgtccgtcca ggccattggc
     6181 gacgtgctca tcggttcgaa cgagcgtcgt ctgattttcc tcaaagacat cgcgaccatt
     6241 caccgggcct acgaggaggt tgcaaacaag ctcatcttct tcaacggtaa gccggcgctg
     6301 actctgggcg tgtccatcca ggccggcgag aacgtggtcg ccgtgggcga ggccgtgaga
     6361 aagcgattcg ggcagctggc gagcacgata cccatcggca tggatatcaa agtcatctac
     6421 aaccagcccg aggaagtgca gaagtcggta tccggcttca tcgtcagcgt cgggcaggcg
     6481 atcgccatcg tgatcgccgt gctgctggtc ttcatggggc tgcgcaccgg catcatcatc
     6541 ggcgccgtgc tgctgatcac cgtctccggc acgttgctct tcatgcagct ctacggcatc
     6601 gagctgcagc ggatttccct gggcgccctg gtgatcgcgc tgggcatgct ggtggacaac
     6661 gccatcgtgg tagcggaagg catgctcgtt cgcatccagg ccggcatgaa ccccaccaag
     6721 gccgccgagg aaacggtcgg caagaccatg tggccgctgc tcggcggcac catcatcggc
     6781 atcctcgcgt tttccgccat cggcctgtcg acagacaaca cgggcgagtt cgcaagctcc
     6841 ctgttctacg tgatcctgat ctccctgacg ctgtcctggg tgacggcggt cagcacgacg
     6901 cccctcctgt gcgccatgtt cttgaagccg gatcccgatg ccgatcctga tgcgaacccc
     6961 tatgacaagg gtttcttcag aaccttccgc ggcgcgctga aggtggtcat ccgcctgcgg
     7021 gtgctcgcgg tggccgtggt ggtcggtctg ttcgtgctgg cgggtttcgg cttcgggtac
     7081 atcaagaacg ccttcttccc gaactcgaac acgcccatgt tcttcgtcga catctgggaa
     7141 cccgagggca ccgacatacg cgtcacccga cgcgacgccc aggcaatctc ggaattcttg
     7201 agagcccagg aaggcgtgcg atccacggtc gccctgatcg gcggcggcga cgcccggttt
     7261 gcgctgacct acgagcccaa ggaatccagc cccgcctacg cccagatcat cgtgcagacc
     7321 gagaccctgg agcagatctc gaaaatctgg ccggcggtag aggcccactt gcaggagcac
     7381 tacccgcacc tggatcccat catcaaggcc tttcgaatcg gcccggggcg cgacggcaag
     7441 atcgaggcgc gattccatgg cccggatcct gcggtgttgc gctccctggc cgagcgggcg
     7501 aaagccatca tgcgcgagga tccgggggcc aaggagatca acgacgactg gcggcagccc
     7561 gtcatgctgg tgcgcccggt gttcaacgag cgggtcgcga ggcagctcgg cgtcacgcgg
     7621 gagaacgtcg ccaccgcgct caagtccgcc tacgaaggga cggacgtggg cgtctaccgg
     7681 gacggcatcc gcatgctgcc gatcaagctt cgcccgccgg cggaagaacg cgccgacgtg
     7741 gcgaacatcc aggacatccg ggtatggagc ccggcgctgc agacggcggt tcccatcgct
     7801 caggtagtga actcgttcga gaccgtgttc gagaacacga ttctgcgcgg ccgaaaccgc
     7861 atccagacca tcatcgccgc ctgcaacccc acgggggagc tggccacgcc gctgttcgag
     7921 cggctcaagc cccggatcga agacatcgag ctgccgccgg gctacgagct gtcctggggc
     7981 ggcgagtacg aggactcggc caacgcccag gccggcctcg cccgggtgct gcccgcgggc
     8041 ttcatcctca tgatcctcac cagcatcctg ctgttcggta aagtccgcca gccgctgatc
     8101 atctggctca cggtgccgct cgccatcatc ggcatcaccg cagggctcct cggtttcgac
     8161 ggcgcgttcg acttcatgtc actgctcggc gcgctgagcc tcatcgggct cctcatcaag
     8221 aacgccatcg tgctgatcga ggagatcgac cagcagatcg cagacggcaa agagcacctg
     8281 accgccatcc tggactccac ggtgagccgt ctgcgtccgg tggtgatggc ggcgtccacg
     8341 accatcctcg gcctgatccc gctcctgcag gacgttttct tcgtcaacat gtccatcacc
     8401 atcatggccg ggctcgggtt cgcgacgttc ctgacactgc tcttcgtacc cgtgctgtac
     8461 tcgctgttct tcaaggtcgc ttaccgtgaa gacgcttgag atcgctggag gaaacatgcg
     8521 cgcaattctg gtcaccgtgg ggctgcttct ggtggccggc tgcgcgaccc agaccacggt
     8581 ggttcgggcc gatttcaccc agggcgaggg cgcctacgtc gtggggtaca gcggggatcc
     8641 ggtgatccgg acgcgcatgg aagagcagct ggtcaacgat ctcagggcac gggagatgat
     8701 cgcgtttgcc agccacaccg acattccgga cattaccgcg agcagccgct acgagctcat
     8761 cgcggcagcc cgcaagcgtg gtgtgatcag cgtcatcgtg atcaaccagg tctcaggcga
     8821 cgcttccgac agcgtcgtcc agaaccccga gcggatctca cccacgcatc cggacctgca
     8881 ggcattctac gactattccc ttggccggca gccggtcgtc gagccgggcc agcaggtttt
     8941 tgccgaggtc aacctgttcg tcctggacgg ggatgaggcc agcctgttct ggagcgggac
     9001 cacctggtct ttccaggccg acggtcaggg caccgccatt cgcggcatca gcgaaaccat
     9061 cgccgatcag ctgcagaagg tgcgggatca ggcacgggca aatcccttca cccagtgagg
     9121 cggacccgtg gtctcggcgt ctaccgcggg acgcagcttc ccagtccgac cacgccgagg
     9181 ccttcacccg ccaccatgat ggtcatcacg ccatcagggt tgttgatgga gacgctccat
     9241 gccacgccgt cgcgctcctc cggcaccccg tcctgaatgc ccgacatgaa gagcttcccg
     9301 tcgagcagct ccatgcggtc gatgtcgttg ggctgggcgt tggggttggt gcccgtgatg
     9361 gttttcttct tggcattgac gatccaggaa ctcgaagcgc caagctcggc gggggtgacc
     9421 gccgtgcagc cattggccat gtcgcactcg aacatgcgaa acgccgagca gagcagatcc
     9481 tgcttgccgt cgaactgagc tgccgcagcc gggacgcctg agagggcc
//
LOCUS       DBNHLM010000007        15904 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_838923, whole genome shotgun sequence.
ACCESSION   DBNHLM010000007 DBNHLM010000000
VERSION     DBNHLM010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 15904)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15904)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15904
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_838923"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="AC16QF_00250"
     CDS             <1..380
                     /locus_tag="AC16QF_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447009.1"
                     /translation="VFRVNMTPVTRPPEFDAGDVARGETASQIQIVIGYQVLVIVPPV
                     DPRAVVDYGRSGSSATFKNSGNSNYLLTDGRQCDPDAPEVCMDLVSYRVYPGNHRQVE
                     LPFDGPFSYKIRTVAGITSQRFE"
     gene            460..2928
                     /locus_tag="AC16QF_00255"
     CDS             460..2928
                     /locus_tag="AC16QF_00255"
                     /inference="COORDINATES: protein motif:HMM:NF027306.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CS1-pili formation C-terminal domain-containing
                     protein"
                     /protein_id="HIR6447010.1"
                     /translation="MLATGAVRATEGGPANVDEQPVVAAVPTRLHPAPIPPARRDTWI
                     PPGFEDLAAPQTTLVDVFYGGDFLISTMVRFSLDEVVILHPEAVADRLPDLIDPRGFT
                     EKLARAFYPNSELLCRSKYQQDCELIETDDVDLIFDRHRLQLSLFIGSPLLRTRTTEQ
                     MKYLPASDAGASIMNDTAAYYSGFVGEDLTYNVASESVFAFAESRISARSNLTDTHGL
                     EVDELAFRREFRGRDLQLGLFRSNIGGFTFLQNQQFIGVNAESSLTTRANVDYALASD
                     VTVFLHSRSRVEIFKDGRLLQSGFYNPGNQVLDTSNLPSGAYDVEIRITDSAGRVTTQ
                     PYYFSKTPRLPPTDQALYFFQAGRETVGGANDFFPETADQGFVRAGLRKRLGASTGGG
                     AALSLNGDSVLVESSLFKQGRNLQAELSAAFESLGIAGVDLGLRYSWRGANLAASARR
                     VWHVEQQPVAQEAMLVDDPAQIGAATTQYSTTFSMPAWKGRFGGFFRYSERDGSALQN
                     YGLNWSASGWSVAGGTLAANIEVSRNDGSVFGAIRLSYRFSREAWSGGVSATLEGNKA
                     SDRAFGVDPRGRAEMRWRSDESFYNDYEFALAASSESERDVEAYASVASDMGTAETSV
                     RWIDSTSRTEIRGQLGTRFAATPQSLSFGGRGHAQSAVLVGVDAGEGSDAEFEVLVDT
                     VKRATLRAGQTRLIALGAFRTYDVEVRSVGDTFASLNKTKERTTVYPGNVLSLNWKAK
                     RIFVGMGRLVDKDGEPIRNALIKNASGLATTDEYGFFQAELDQSAEMLAVQKRGSSCL
                     VDLGEFEVVDQVSYLGTLTCTMSS"
     gene            complement(3133..3208)
                     /locus_tag="AC16QF_00260"
     tRNA            complement(3133..3208)
                     /locus_tag="AC16QF_00260"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3173..3175),aa:Phe,seq:gaa)
     gene            complement(3325..4215)
                     /locus_tag="AC16QF_00265"
     CDS             complement(3325..4215)
                     /locus_tag="AC16QF_00265"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR6447011.1"
                     /translation="MPMGRPQAERVVDEALVRRLLEDQHPDLADLPLTHLDSGWDNVI
                     YRLGDKLTVRLPRREVAAALLHHEQTWLPRIADGLPLPVPSPVAMGRPTAYYPWPWSV
                     LPWFEGESADLSPPRAEQADRFADFLIALHRPAPADAPANPVRGVPLMAREANTRERM
                     GRLNSKSDALSPALESIWESALTAPQAGEACWLHGDLHAQNVLVNGTGSLTAIIDWGD
                     ITRGDPATDLAGIWAVFPTRRARQSILQAYRPDTALLDRARGWAFVFGVVLTDSGNVN
                     SPRHAAAGRRILERLEADSG"
     gene            complement(4226..4498)
                     /locus_tag="AC16QF_00270"
     CDS             complement(4226..4498)
                     /locus_tag="AC16QF_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HIR6447012.1"
                     /translation="MSRTVTCRKYGEKLEGLDAPPMPGPKGQEIFETVSRRAWQEWQD
                     LQTMLINERALDMRDPEARKYLNAQRERFLDNEETDHAEGHVPRSS"
     gene            complement(4495..5562)
                     /gene="mutY"
                     /locus_tag="AC16QF_00275"
     CDS             complement(4495..5562)
                     /gene="mutY"
                     /locus_tag="AC16QF_00275"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIR6447013.1"
                     /translation="MRPDWFTKRVLKWFDLHGRHDLPWQEDIDPYRVWVSEIMLQQTQ
                     VTTVIPFYERFIARFPTVAALAGAELDEVLHHWTGLGYYARGRNLHKAARVIAHDLDG
                     RFPVGVEALSELPGIGRSTAGAISAIAQGVRAPILDGNVKRVLARFHAVAGYPGQTAT
                     QKSLWELAEAHTPNTRVADYTQAIMDLGATVCTRQPRCEVCPVAERCDALAQGATDDF
                     PGRKPKRSKPTRTARMFLIVAPDGACLLEQRPAKGLWGGLWIPPQRDPDCDVPAVLRE
                     FDIPESAVAESRVEPGFRHTFTHFHLDIEPVHVRLRARPLPGVRDGTDCLWYRREDDT
                     EVGLSAPAVALLDSLKEFELA"
     gene            complement(5565..7079)
                     /locus_tag="AC16QF_00280"
     CDS             complement(5565..7079)
                     /locus_tag="AC16QF_00280"
                     /inference="COORDINATES: protein motif:HMM:NF017021.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR6447014.1"
                     /translation="MVKRILMILLWIVLLGGGFVFYWIQDANRIKPELEQLIEEQAGI
                     PVTIGGDLSWRLFPPLTLQAEQIAASYEGQDWTAERLSLDIDVMTVLRTRDVNRWRVQ
                     SLTLTNTVGRDARTEIDIKSLTLREFELGKPSPLEISMVYTGEGAEPLPVEMTGLVTV
                     QSGPDRYEITNADLSTPYADGVCNLEASPASSSAPLPPATLEDLIPVATWRAFDWSGR
                     CDLSRLALDDLTFDSATVDLANTAANSATRITIPEFFGGMAILELQIDASGTPVRWIL
                     TPDLTGVDSQRLMAWLDQRLEWMAPLAYNGTLKFEGNTADALIASLSGETRFDGGRGA
                     ISIIKIKQPLLAVATMLQEPGRVSGWPDLWQYDRLEGVWRADRQHHTLDFALDNLSVI
                     ADGNYDPAADRLDMMAELTFRTLTEGKMFELNPVLMDLPIPVRCRGSLENPECRVDQK
                     AAQRIVASVLTSKEGNEAREKLEEKIDEEVPEEYRDAARGLLDLLGRSLQDKNQ"
     gene            7191..7589
                     /locus_tag="AC16QF_00285"
     CDS             7191..7589
                     /locus_tag="AC16QF_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447015.1"
                     /translation="MTVESFDPSAVDHTISDSRLARLLDAAGCLDEESFGLGTEEVRD
                     LAAVARHPESDWSERAGTLGDEEVLALIRLFTLAETRLSGWESGAASPVVPLAAALKR
                     RDAYPSDLNRWIRSNTANRFLPYGSLMDRL"
     gene            7648..8259
                     /gene="hisB"
                     /locus_tag="AC16QF_00290"
     CDS             7648..8259
                     /gene="hisB"
                     /locus_tag="AC16QF_00290"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIR6447016.1"
                     /translation="MATKTKPSGRSVTVSRDTQETRIAVSVDLDGSGASRLTTGLAFF
                     EHMLTQIARHGLIDLDIRADGDLEVDAHHTVEDVGIVLGQAIGQAVGDKAGISRYGHA
                     YVPLDEALSRAVVDFSGRPGLTYEVDFVRPRIGDFDVDLLREFFQGFVNHAQVTVHLD
                     NLRGENAHHQAETLFKAFGRALRMAVAPDPRMAGQVPSTKGSL"
     gene            8260..9003
                     /gene="hisA"
                     /locus_tag="AC16QF_00295"
     CDS             8260..9003
                     /gene="hisA"
                     /locus_tag="AC16QF_00295"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR6447017.1"
                     /translation="MLLIPAIDMKDGQCVRLRQGRMDDATVFSDDPLAMAAHWAEQGC
                     RRLHLVDLDGAFAGEPRNGALVAAIARELGEIPVQIGGGIRSLDIIERYLDAGVSQVI
                     LGTRAVQDPDFLASAAERHPGRIILGLDARNGMLATDGWDKTTQTSAVGFAMGASRLD
                     LAAIVYTDIERDGMLEGLNLAATVALAEAVETPVVASGGISCLDDLAALKAAFAGSSG
                     RLMGAITGRAIYAGTLDFRAGQALLDGSG"
     gene            complement(9013..10197)
                     /locus_tag="AC16QF_00300"
     CDS             complement(9013..10197)
                     /locus_tag="AC16QF_00300"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR6447018.1"
                     /translation="MNLRPITLVGIAISMVLGIGVGIGAYRAWLEPDVNGRHGQAFDE
                     VLRQVHEGYVDEVAKEKLLENALKGMLHGLDEHSDYLDHLSYEDLQAETSGQFGGIGI
                     ELNLVDGYFTVIRPITDTPASAAGLQPGDRLTEIDRESLKGKKMVDIISSLRGTPGTP
                     VILGIRRDGGQKMEVSLIRAVIQDPSVDGRLLEPGYGYVRISQFQVSTGSRFTSAIRE
                     LAHQNGESLRGLVLDLRDNPGGVLQSSVDVADALLEEGLIVYTEGRLPSSYLKYRATS
                     GDILDGAPVVILINGGSASAAEVVAGALQDHGRAVVLGAKSYGKGSVQSVMPLSEDEA
                     IKLTTAYYYTPQGRSIEDTGIVPDVAVDEASGDDDDSGLLLGQALDLLKAEQGGGLHA
                     RL"
     gene            complement(10220..11404)
                     /locus_tag="AC16QF_00305"
     CDS             complement(10220..11404)
                     /locus_tag="AC16QF_00305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR6447019.1"
                     /translation="MNSLPTTDWLPRSRAGRRAAGTRRLAIVVLSLLAPLSFSSEKED
                     AQRELEDVVGKLNALDVWLDEAEERRVRWLGEVKIKDREVASVSEAVDEATAALAVVI
                     RSLGKLEAEQRELQEKRERQATKIGEHLAAAYRLDGRDFVQLLLNQESPDSFDRMIRY
                     HRYFSSARLSALQEYQETLEALAANRLALEDRAAEQEARKAELAERQARLIEERDQRR
                     LLIAQLDAEAEDKAVQRQRLDTDRDRLERLLDELNRRAMELDGSAFVARKGSLPWPLK
                     GPLRSSFGRPRADGRMVWQGMLVAADEGSPVKAVFRGRLIFADWLRGFGLLAIINHGD
                     GYMTLYGHADSLTKTVGDWVEAGEVIARAGRSGGQQASGLYFEVREEGVPRDPLGWLA
                     KR"
     gene            complement(11401..12954)
                     /gene="gpmI"
                     /locus_tag="AC16QF_00310"
     CDS             complement(11401..12954)
                     /gene="gpmI"
                     /locus_tag="AC16QF_00310"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR6447020.1"
                     /translation="MGNPTLLVVLDGFGYSPSTQDNAIAQARTPNWDRFWNEKPHALI
                     SGSGADVGLPDDQMGNSEVGHMNLGAGRKVYQDLTRINRAIEQGEFARNRTLNRTLQR
                     LRKAGGRLHVMGLLSPGGVHSHEDQIFEFIRMAAAAEIQHIYLHAFLDGRDTPPRSAE
                     PSLARADALLAELGAGHVASVTGRYYAMDRDRRWDRIERAYNLLVRGEAPFHAANGRA
                     ALAAAYERDESDEFVQPTAVHAEGELPVRMLDGDAVVFMNFRADRARQITRAILEPGF
                     DGFQRRSTVALSDFVMLTRYADDIDTSCAFEPAVLSNTLGEYLANLGKTQLRIAETEK
                     YAHVTFFFSGGREDPFAGEERLLIPSPKVATYDLAPEMSARKVTDHLVEAIASKQHDL
                     IICNFANGDMVGHTGVFDAAVKAVETLDECLGRIETAIVEAGGQMLITADHGNVEQMR
                     DHGTGQAHTAHTCEPVPLVYIGPQSVKLNSGGTLSDVAPTLLELMHLEKPAEMTGHSV
                     AQLEERRSA"
     gene            13099..13515
                     /locus_tag="AC16QF_00315"
     CDS             13099..13515
                     /locus_tag="AC16QF_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR6447021.1"
                     /translation="MDQLFEFIVNHPVLVGAFVVLLVFFVRNETQRGGRSVSAQELVD
                     LVNREDAVVLDVRDKKDFDQGHIPHALNIPYASLDGRIAELNAHKEKPIVIACKMGQH
                     AGAAGTALRKQGFANVTRLKGGIAEWRNQNLPVVKA"
     gene            13479..13961
                     /gene="secB"
                     /locus_tag="AC16QF_00320"
     CDS             13479..13961
                     /gene="secB"
                     /locus_tag="AC16QF_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HIR6447022.1"
                     /translation="MAESEPAGGEGVARGQIRLERLYLKDASFESPRSPAVFGESWKP
                     EMQLDINTRTNPQEGDRFEVVLTVTLRAKLDDGRTAYIIEVQQAGLFVIQGAEDAVVH
                     RVLGTMCPNTLFPYVRETLDSLAVRGGFPAIQLAPVNFDALYEEALKQRAPPANPVQH
                     "
     gene            complement(13963..14430)
                     /gene="trmL"
                     /locus_tag="AC16QF_00325"
     CDS             complement(13963..14430)
                     /gene="trmL"
                     /locus_tag="AC16QF_00325"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HIR6447023.1"
                     /translation="MPLHVVLYQPEIPPNTGNVIRVCANTGARLHLVEPLGFEMDDSR
                     LRRAGLDYHEFACVDVHASLDDALDACGGGRCYAVSTRGSRRFSEITFTDGDVLLFGP
                     ETRGLPADVLETFPAAHVLRIPMRAGSRSLNLSNAVAVVVYEAWRQLGYAEGT"
     gene            complement(14434..15834)
                     /gene="ntrC"
                     /locus_tag="AC16QF_00330"
     CDS             complement(14434..15834)
                     /gene="ntrC"
                     /locus_tag="AC16QF_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HIR6447024.1"
                     /translation="MRNRVWVVDDDRSIRWVLERAMQQADIPIAAFASADHAMHHLQD
                     ERPVAVLTDVRMPGMDGMEFIGRIHEAYPELPVIIMTAHSDLDSAVNAYQSGAFEYLP
                     KPFDVDEAVAIVKRAMTFAQEEAPPPAPEQETAQEIIGQAPAMQEVFRAIGRLAHSNV
                     TVLIRGASGSGKELVARALHRHSPRAEGEFVALNMAAIPRELVESELFGHEKGAFTGA
                     NSRREGRFEQADGGTLFLDEIGDMPAETQTRLLRVLADGEFYRVGGHSPVKVDVRIIA
                     ATHQDLEVRVRDGSFREDLFHRLNVISVHVPTLAERRTDIPALASHFLEQAAEELGGE
                     PKIIRPDTMEYMMNLSWPGNVRQLENTCRWLTVMASGREIYLADLPPELGSDVVEQDT
                     NDSWEQALEAWADDLLREPGGLPLLDVAMPRFESVVIRAALRRTGGRKREAAELLGWG
                     RNTLTRKIAELGMTEL"
BASE COUNT         2597 a         5097 c         5265 g         2945 t
ORIGIN      
        1 aagtatttcg ggtgaacatg acgcccgtga ccaggccgcc ggaattcgat gcgggagatg
       61 ttgcacgagg cgaaaccgcc tcgcagatcc agatcgtgat tggttatcag gtcttggtga
      121 tcgttccgcc ggtagatccc cgggcagtgg ttgactacgg ccgctcggga agctccgcga
      181 ccttcaagaa ttccggaaac tccaactacc ttctcaccga cggtcgtcag tgtgatcctg
      241 acgcgccgga ggtttgcatg gaccttgttt cctatcgggt ttatccgggc aaccacaggc
      301 aggtcgagct ccccttcgac gggcccttca gctacaagat tcggacggtg gcgggcatca
      361 ccagccagcg cttcgagtag ttgcacggcc ttcgctcgct agatgtttcg caggccgccg
      421 acgtggcaag tagcctggac cctggtcgcg gcaaccctga tgctcgcgac cggcgccgtc
      481 cgggcgacgg agggcggccc tgcaaacgtc gacgagcagc cggtggtcgc cgccgtgccc
      541 acccggctgc atccggcgcc gattcccccg gctcgtcggg atacctggat tcccccgggt
      601 ttcgaagacc ttgctgcgcc gcagaccact ttggtggacg tcttctacgg cggagatttc
      661 ctcatttcca ccatggtccg cttcagcctc gacgaggtcg ttatcctgca tccggaggcg
      721 gttgccgacc gcctgcctga cctgatcgac ccacggggat ttacggaaaa gctcgctcgg
      781 gccttctatc cgaattccga gctgctgtgc agatccaagt atcagcagga ctgcgaactc
      841 atcgaaaccg acgacgtcga cctgattttc gatcgccacc gccttcagtt gagcctcttc
      901 atcggttcgc cgctgcttcg gacgcgcaca acggagcaga tgaaatacct gcccgcgtcc
      961 gatgcagggg cctctatcat gaatgacacg gccgcctact acagcgggtt cgttggcgaa
     1021 gacctgacct acaacgtcgc cagtgagagc gtcttcgctt ttgccgagag tcggatctcg
     1081 gctcgcagca acctgacgga tactcatggt ctggaggtgg acgagcttgc gtttcgtcgt
     1141 gagtttcgcg gcagggacct tcagctcgga ttgtttcggt cgaacatcgg cggttttacc
     1201 tttctgcaga atcagcagtt catcggcgtg aacgcggaga gctccctcac cacccgagcc
     1261 aatgtggact acgccctcgc gagcgacgtc accgtcttcc tgcacagccg cagccgggtg
     1321 gagatcttca aggacggccg gctgctgcag agcggttttt acaaccccgg caatcaggtc
     1381 ctggatacct cgaacctgcc gtccggtgcg tacgacgtgg agatacgcat caccgactcg
     1441 gctggccggg tcaccacgca gccctactac ttttccaaga cgccgcggct tccgcccacg
     1501 gatcaggcgt tgtacttctt tcaggccggg cgcgaaaccg tggggggagc caatgatttt
     1561 tttccggaaa ccgcggatca ggggtttgtc cgtgccgggc tgcgcaagcg actgggagcg
     1621 tccaccggtg gcggcgcggc gctttccctg aatggcgaca gcgtgctcgt cgagtccagt
     1681 ctcttcaagc agggacgcaa tctgcaggcg gagctcagtg cggcattcga aagcctgggc
     1741 attgcggggg tggacctcgg cctgagatac agctggcggg gcgccaatct cgccgcttct
     1801 gcccggcgtg tctggcacgt cgagcagcaa ccggtagctc aggaagccat gcttgtcgat
     1861 gaccccgccc agatcggcgc cgcgacgacc cagtacagca ccacgttttc tatgccggcc
     1921 tggaaagggc gcttcggcgg ctttttccga tactccgagc gcgatggctc tgccctgcag
     1981 aactacgggc tgaactggtc ggccagcggt tggagtgtcg ccggaggcac ccttgccgcc
     2041 aacatcgaag tttcccggaa cgatggctcg gtgttcggcg cgattcgatt gagctatcga
     2101 ttctccaggg aagcgtggtc cggtggcgtc tcggctacgc tcgagggtaa caaggcttcg
     2161 gacagggcgt tcggtgtgga cccgcgcggt cgggcggaaa tgcgctggcg cagtgacgag
     2221 tccttttaca acgactacga gttcgcgctg gccgcttcgt ccgagtccga gcgcgacgtc
     2281 gaagcctacg caagcgttgc ctccgacatg ggcacggcgg agacgtcggt gcgctggatc
     2341 gactcgacga gccgcaccga gattcgcggc cagctgggca cccggttcgc tgcaacccca
     2401 caatcactga gctttggcgg ccgtggccat gcgcagagcg cggtgcttgt gggggtcgat
     2461 gcgggggaag ggtccgacgc ggagttcgaa gtgctggtcg atacggtcaa gcgcgccacg
     2521 cttcgggccg gccagacccg gctgattgcg ctcggcgcct tcagaaccta cgacgtcgag
     2581 gtgcggtcgg tgggggatac ctttgcctcc ctcaacaaga cgaaagaacg cacgaccgtg
     2641 tatccgggca acgtgctgtc gctcaactgg aaggcgaagc gcatcttcgt cggcatgggc
     2701 cgactggtcg acaaagacgg cgagccgatc cgcaacgcgc tgatcaagaa cgcatcgggc
     2761 ctggcgacca cggacgaata cggctttttt caggcggagc tcgaccaaag cgccgagatg
     2821 ctggcagttc agaaaagggg ttccagctgc ttggtcgacc tcggcgagtt cgaggtcgtg
     2881 gatcaggttt cctatctcgg taccctgacc tgcacgatgt cgtcctaaca gcttctgcct
     2941 cgtctggtgg ccgctggcca ctgcgcctct accttaggtc tcgtctggtg gccaggggga
     3001 gaatcgacgt tgcgaagcag ccgaccgcgg agcgtggcgg catcagcgag agtggtgaat
     3061 cgaagtgaag tgcgcgtttg gagctcaaca atggtgtccg ctggccactg cgcctcgacc
     3121 ttaggtctcg tctggtggcc aggggcagaa tcgaactgcc gacacgcgga ttttcagtcc
     3181 gctgctctac caactgagct acctggccgg aaagcttgct ggggcgggcc ctttcactgc
     3241 gtcctgggac aggctttctt gtttggtccc cagcaaaggg gcggtattaa accgatctga
     3301 cccttaaact gcaaggcgtc gcggctagcc ggaatccgcc tccagccgct cgaggattcg
     3361 cctgccggcg gcagcgtgcc gggggctgtt gacgttcccc gaatcggtca ggacgacgcc
     3421 gaatacgaag gcccaccccc tagctcggtc gagcagtgcg gtatccggcc gatatgcctg
     3481 gagaatgctc tgccgcgcac ggcgcgtggg gaaaacggcc cagatgccgg ccagatcggt
     3541 ggccggatcg ccccgggtga tgtctcccca gtcgatgatt gccgtaaggc tgccggtgcc
     3601 gttcaccagc acgttctgag cgtgcaggtc gccgtgaagc cagcatgcct cgccggcctg
     3661 cggcgctgtg agcgccgact cccagatcga ttcgagggcg ggggagagcg cgtcgctttt
     3721 cgagttcagg cgccccatac gctcgcgggt attggcctcg cgcgccatga gcggtacccc
     3781 ccggaccgga ttagccggag cgtctgccgg agcggggcgg tgcagggcga tgagaaagtc
     3841 ggcaaagcgg tcggcctgct cggcgcgcgg cggcgacaaa tcggcgctct ccccctcgaa
     3901 ccagggcaga acgctccagg gccaggggta gtaggctgtc ggtctgccca tggcgaccgg
     3961 gctcggcacg ggaaggggaa ggccgtctgc gatgcggggc aaccaggtct gctcgtggtg
     4021 gagcagcgcc gcagccacct cccgccgggg caggcgcacc gtgagcttat cgcccagccg
     4081 gtagatcacg ttgtcccagc cggagtccag atgcgtgagc ggcagatcgg cgaggtccgg
     4141 gtgctggtcc tccagcagcc gtcgcaccaa agcctcgtcg acgacccgct ctgcctgggg
     4201 ccgccccatg ggcatggtct gctgctcagg aagatcgggg cacatggccc tcggcgtggt
     4261 cggtctcctc gttgtccaga aaccgttccc gctgggcgtt gagatacttt cttgcctcgg
     4321 ggtcgcgcat gtccagcgct cgctcgttga tcagcatcgt ctgcaggtcc tgccactcct
     4381 gccaggctct gcgcgagacg gtttcgaaga tctcctggcc cttgggtccg ggcataggcg
     4441 gcgcgtcgag accctcgagc ttttcgccgt acttgcggca ggtgaccgtg cggctcatgc
     4501 cagctcaaac tccttaaggg aatccaatag tgcaaccgcg ggagcggaca gtccaacctc
     4561 ggtgtcgtct tctctccggt accacaggca gtccgtgccg tcgcgaacgc cgggaagcgg
     4621 tctcgcgcga aggcggacgt gcacgggctc gatgtcgaga tgaaagtggg tgaacgtgtg
     4681 gcgaaaccca ggctcgaccc ggctttcggc aacagcggat tccgggatgt cgaactcccg
     4741 gagcaccgct ggaacgtcgc agtcggggtc ccgctgcggc gggatccaca atccgcccca
     4801 caggcccttg gccggtcgct gctccaacag gcacgccccg tctggggcga cgataagaaa
     4861 cattctcgcc gtgcgcgtcg gcttggaccg tttcggcttg cgcccgggaa agtcatcggt
     4921 tgcgccctgg gcgagggcat cgcagcgctc ggccacgggg cagacctcgc accggggctg
     4981 gcgagtgcac acggtagcgc cgaggtccat gatcgcctgg gtgtaatcgg cgacacgcgt
     5041 gttcggggtg tgggcctcgg cgagctccca gagcgacttc tgcgtggccg tctggccggg
     5101 gtagcctgcc acggcgtgga agcgggctag cacccgtttg acgttgccgt cgagaatcgg
     5161 cgcgcggact ccctgggcga ttgcggagat ggcgccggcg gtagaacgac cgattcccgg
     5221 cagctcgctc agcgcctcga ccccgacggg aaaccggccg tcgagatcgt gcgcgatgac
     5281 ccgggcggcc ttgtggaggt tgcggcccct tgcgtagtag ccgagccccg tccagtggtg
     5341 caggacctcg tccagctccg ccccagcgag tgctgcgacc gtcgggaaac gggcaatgaa
     5401 acgctcgtag aacgggatca cggtggtgac ctgggtctgc tgaagcatga tctcggagac
     5461 ccatacccgg tacggatcga tgtcctcctg ccacggcagg tcgtgccggc cgtgcaggtc
     5521 gaaccacttc agaacgcgtt tcgtgaacca gtcgggcctc accgttactg gttcttgtcc
     5581 tgcaatgatc tgcccaggag atccagcagg ccgcgggctg cgtcccggta ctcctcaggg
     5641 acctcttcgt cgatcttctc ctcgagcttc tctctcgcct cgttgccctc tttgctcgtc
     5701 agcacggagg ccacgatgcg ctgggcggct ttctgatcta cccgacattc ggggttttcc
     5761 aggctgccgc gacagcgaac gggaatgggc aggtccatca ggaccgggtt cagctcgaac
     5821 atctttcctt cggtcagcgt cctgaaggtc agctccgcca tcatgtcgag ccgatcggcg
     5881 gcaggatcgt agttgccgtc tgcgatgacc gacagattgt ccagagcgaa gtccagggtg
     5941 tggtgctgcc ggtccgcccg ccacacgccc tcgagacgat cgtattgcca cagatcgggc
     6001 catccggaga cccggcccgg ctcctgcagc atggtggcga cagccagaag cggctgcttg
     6061 atcttgatga tggagatggc gccccgccca ccgtcgaaac gcgtctcgcc ggaaagcgag
     6121 gcgatgagcg cgtcagccgt gttgccttcg aacttcaggg tgccgttgta ggccagcggc
     6181 gccatccact ccaggcgctg atccagccag gccatgagac gttggctgtc caccccggta
     6241 aggtcggggg tcaggatcca gcgaacgggc gtgccgctgg cgtcgatctg cagctcgagg
     6301 atggccatgc cgccgaagaa ctcgggaatg gtgatccgcg tggcgctgtt tgcagcggtg
     6361 ttggcgagat cgaccgtggc ggagtcgaag gtgaggtcgt cgagcgcgag gcgcgacagg
     6421 tcgcatcggc cggaccagtc gaaggcgcgc catgtggcga cgggaatgag atcctcgagg
     6481 gtcgccggtg ggagcggggc gctgctgctg gccgggcttg cctcgaggtt gcagacgccg
     6541 tcggcgtagg gagtggatag atccgcgtta gtgatctcgt atcggtcggg tccggactgg
     6601 acggtcacga gcccggtcat ctccacgggc agcggctcgg ctccttcccc ggtatagacc
     6661 atcgagatct ccagcggcga cggcttcccc agctcgaatt cccgcagggt gagggatttg
     6721 atgtctatct ccgtccgggc gtcgcggccg acggtgttcg tcaaagtcag cgactgcacc
     6781 cgccagcggt tcacatcccg ggtacgcagc acggtcatga cgtcgatgtc caggctgagc
     6841 ctttcagccg tccagtcctg accctcgtag ctcgcggcga tctgctctgc ctgcagtgtc
     6901 aggggcggga acagacgcca gctcagatcc ccgccgatgg tgacggggat gccggcctgc
     6961 tcttcgatga gctgctccag ctccggcttg atccggttgg cgtcctggat ccagtaaaag
     7021 acgaagccgc caccgagcag cacgatccac aggaggatca tgagaatgcg tttgaccatg
     7081 tggtgttgcc gtgagtctgg gaagtgagga cgattataag gatttcgggc tggccggcag
     7141 ctgaacggcg cgtttgctat agtctcgccc ccgcagacca tctggccgcc atgaccgtag
     7201 aatccttcga cccctcagcc gtggaccaca cgatttccga ttcgcgtctc gcgcgcctgc
     7261 tcgatgcggc cggttgcctc gacgaggaga gcttcggtct cggtaccgag gaggtgagag
     7321 atctcgcggc cgttgcccgc catccggagt cggactggtc cgagcgggcg gggacgctgg
     7381 gagatgagga agtcctggcc ctgatcaggc tgttcacgct ggcggaaacc cgcctgtcgg
     7441 gctgggagtc cggagcggca tcgcccgtgg tcccgctggc ggcggcgctc aagcgccgcg
     7501 atgcctaccc gtcagatctc aaccggtgga tcaggagcaa cacggcaaac cgctttctgc
     7561 cctacgggag cctgatggac cggctgtagt cgtcgggttc gagtccgtat acttgcgcgg
     7621 cctcactgaa gcggaaggcg gttgatcatg gccacgaaaa ccaagcccag cggtcgctcg
     7681 gttaccgtct cccgggatac ccaggagacg cggatcgccg ttagcgtgga tctggacggc
     7741 tcgggcgcgt ccaggctgac cacggggctc gcctttttcg agcacatgct cacgcagatt
     7801 gcccgtcatg ggctcatcga tctcgacatc cgcgctgacg gcgacctcga ggtggatgcc
     7861 caccacacgg tggaggacgt tggcatcgtc ctgggccagg ccattggtca ggccgtcggc
     7921 gacaaggccg gcatctcgcg ctacgggcat gcttacgttc ccctggatga agcgctctcc
     7981 cgtgccgtgg tggacttttc cggccgcccg ggcctgacct acgaggtgga tttcgtgcgg
     8041 ccccgaatcg gcgatttcga cgtcgacctc cttcgcgagt ttttccaggg attcgtcaac
     8101 cacgctcagg tgacagtgca tctggacaat ctgcgcgggg agaacgccca ccatcaggcc
     8161 gaaacgcttt tcaaggcgtt cggccgggcg ctgcgcatgg ccgtcgcccc ggatccgcgc
     8221 atggccgggc aggtgccgtc caccaaaggc agcctctaga tgctcctgat cccggccatc
     8281 gacatgaaag atggccagtg cgtgaggctt cgccagggtc ggatggacga cgccacggtg
     8341 ttctcggacg atccgctggc catggccgcg cactgggccg agcaggggtg tcgccggctg
     8401 catctcgtgg atctcgacgg cgcctttgcc ggcgagccgc gcaacggcgc tctggtggcc
     8461 gccatcgccc gtgagctcgg tgagatcccg gtgcagatcg gcggcggcat tcgcagcctg
     8521 gacatcatcg aacgatatct ggacgccggg gtctcccagg tcattctggg gacccgggcc
     8581 gttcaggatc ccgatttcct ggcgtccgca gccgaacggc accctggccg gatcatcctt
     8641 ggcctcgacg ctcgaaacgg catgctcgcc accgacggct gggacaagac gacccagacc
     8701 agtgccgtcg gctttgcgat gggcgcctcg cgactggatc ttgcggccat cgtgtatacg
     8761 gacatagagc gggacggcat gctcgagggg ctcaacctgg cggcgaccgt ggcccttgcc
     8821 gaggccgtcg agacccccgt cgtcgcctcg ggcggcatca gctgcctcga cgatctggca
     8881 gccctgaaag ccgcctttgc cgggagctcg ggaaggctga tgggagccat cacgggtcgc
     8941 gccatctatg cgggcaccct ggattttcgg gctggacaag cccttctcga cggctcgggt
     9001 tgaaggcgcg cgctacaggc gcgcgtgcag ccctccgccc tgttcggctt tgagcaggtc
     9061 gagggcctgg ccgagcagca gcccggagtc gtcatcgtcg ccagaagcct catcgacggc
     9121 aacgtcgggc acgatgccgg tgtcctcgat ggatcgcccc tgaggggtgt agtagtaggc
     9181 ggtggtcagc ttgatcgcct catcttccga cagcggcatt accgactgca ccgagccctt
     9241 accgtaactt ttcgctccca ggacgacggc cctgccgtgg tcctggagag cgcccgcgac
     9301 cacctcggct gcggatgccg agccgccgtt gatcagaatc accaccgggg cgccatcgag
     9361 gatgtcaccg ctggtcgccc ggtatttcag atagctggaa ggcaggcgac cctcggtgta
     9421 cacgatgagg ccctcctcga gcagggcatc cgcgacgtcc acggaggatt gcaggacccc
     9481 acccgggttg tcccgcaggt ccagcaccag gccgcgcagg ctttccccgt tctggtgcgc
     9541 cagctcacga atggccgagg tgaaccggct cccggtgctc acctggaact gcgagatccg
     9601 gacgtaaccg taacctggct cgagcagccg gccgtccacg ctgggatcct gtattacggc
     9661 ccggatcagg ctgacctcca tcttctggcc gccgtcgcgg cgaatgccca ggatgaccgg
     9721 cgtgccgggt gtcccgcgaa ggctggagat gatgtccacc atcttcttgc ccttgagcga
     9781 ctctctgtcg atctcggtca gcctgtcgcc gggctggagc cccgcggcgc tggcgggcgt
     9841 gtcggtgatc ggccggatta cggtgaaata gccgtcgacc aggttgagct cgatgccgat
     9901 gccgccgaac tgcccgctgg tttccgcctg cagatcctcg tagctgaggt ggtccagata
     9961 gtcggaatgc tcgtcgagac cgtggagcat tcccttgagg gcgttttcga ggagcttttc
    10021 cttggccacc tcgtccacgt agccttcgtg cacctggcgc agcacctcgt cgaaggcctg
    10081 gccgtgccgg ccattcacat cgggctccag ccaggcccgg taagcgccga tgccgacgcc
    10141 gatcccgagg accatgctga tcgcgatgcc gaccagagtt atgggacgca agttcatacc
    10201 ggggagtttg gcaccagcat cagcgtttgg ccaaccaccc gagggggtcg cggggcacgc
    10261 cttcctcgcg cacctcgaag tacaggccgc tggcctgctg gccgccgctg cggccggcgc
    10321 gggcgatgac ctcccccgcc tcgacccagt cgcccacggt tttcgtgagc gagtcggcat
    10381 ggccgtatag cgtcatgtaa ccgtcgccgt ggttgatgat ggcgagcagt ccgaagccgc
    10441 gcagccagtc ggcgaaaatc aaccggccgc gaaaaacggc ctttaccggc gagccttcgt
    10501 ccgcggccac cagcatccct tgccagacca ttctcccgtc ggcccgtggc cgtccgaacg
    10561 atgagcgcag gggacccttg agcggccagg gaagtgatcc cttgcgggca acgaaggcgg
    10621 acccgtccag ctccatggcg cgtcggttga gctcgtccag cagacgttcg agccggtcgc
    10681 ggtcggtgtc cagccgctgc cgctggacgg ctttgtcctc tgcctcggca tccagctgcg
    10741 cgatcagaag cctgcgctga tcgcgctcct cgatgagcct ggcctggcgc tccgcaagct
    10801 ccgccttgcg ggcttcctgc tcggccgcac gatcttccag ggccagcctg ttggcggcca
    10861 gcgcctcgag agtttcctgg tactcctgca gggcagacag gcgggcgctg ctgaagtagc
    10921 ggtggtaacg gatcatccga tcgaacgagt ccggggattc ctgattgagc aagagctgca
    10981 cgaagtcgcg accgtccaaa cggtacgcgg cggcgaggtg ctcgccgatc ttggttgcct
    11041 gccgctcccg cttctcctgc agctcgcgct gctccgcctc gagcttgccc agggaccgaa
    11101 tcaccacggc caacgcggcg gtcgcctcgt cgacggcctc gctcaccgag gccacctccc
    11161 ggtccttgat cttgacctct ccgagccagc gcacgcggcg ctcctcggct tcatcaagcc
    11221 atacgtccag ggcgttgagc ttgcccacga cgtcctccag ctctctctgg gcgtcttcct
    11281 tctcgctgga gaagctcaac ggcgccagga gagaaagcac cacgatcgca aggcgacgcg
    11341 tgccggccgc ccggcgcccg gcgcggctcc gcggtagcca gtcggtcgtc gggagactgt
    11401 tcaagcggag cggcgttcct ctagctgagc gacggagtgc ccggtcattt ctgccggttt
    11461 ttccaggtgc atgagctcga ggagggtggg tgctacgtcg gagagggttc ctccggagtt
    11521 cagcttgacc gattgcggtc cgatgtagac gaggggcacg ggctcacagg tgtgggcggt
    11581 gtgagcctgg ccggtgccat ggtcgcgcat ctgctccacg ttgccgtggt cggctgtgat
    11641 gagcatctgc ccgcctgcct ctacgatggc ggtttcgatc cggcccaggc actcgtccag
    11701 tgtctccacg gccttcacgg ccgcgtcgaa cacgccggtg tgaccgacca tgtcgccgtt
    11761 ggcaaagttg cagatgatca ggtcgtgctg cttcgacgct atcgcttcca ccaggtggtc
    11821 ggtgaccttg cgcgcgctca tctcgggcgc caggtcgtag gtggccactt tcggtgaagg
    11881 gatgagcagg cgttcctcgc cggcgaaggg atcctctctc ccgccggaga agaagaaagt
    11941 gacgtgagcg tacttctcgg tctcggcgat gcgcagctgg gtcttgccca ggttcgccag
    12001 gtactcgccg agcgtgttgg acagcacggc cggctcgaac gcgcacgagg tgtcgatgtc
    12061 gtcggcgtac cgggtgagca tgacgaagtc ggacagcgct accgtggagc ggcgctggaa
    12121 accgtcgaag cccggctcca gtattgcccg ggtgatctgg cgggctcggt cggcgcggaa
    12181 gttcatgaag accacggcgt ctccgtcgag catccggacc ggcagctcgc cctccgcgtg
    12241 cacggccgtg ggctgcacga actcgtcgct ttcgtcccgc tcgtacgcgg ccgccagcgc
    12301 tgcgcgcccg tttgccgcat gaaagggagc ctctccccgg accagcaggt tgtacgcgcg
    12361 ctcgatgcgg tcccagcgcc ggtctcggtc catggcgtag tagcgcccgg taacagaggc
    12421 tacgtgacct gcgccgagct cggcaagcag agcgtcagcc cgtgcgaggg acggctccgc
    12481 gctgcgcggc ggtgtgtcgc gcccgtccag aaaggcgtgc agatagatgt gctgaatttc
    12541 cgccgccgcg gccatccgga tgaactcgaa gatctgatct tcgtggctgt gcaccccgcc
    12601 cggggacaac aggcccatga cgtgcaggcg cccgccggct tttctgagac gctgcaaagt
    12661 cctgttgaga gttctgttgc gggcgaactc gccttgctcg atggcgcggt tgatccgggt
    12721 caggtcctga tagaccttgc gaccggcccc gaggttcatg tgccccactt ccgagttccc
    12781 catctggtcg tccggaaggc caacgtccgc ccccgagccc gagatcagcg catgaggttt
    12841 ctcgttccag aagcgatccc agttgggcgt gcgcgcctgg gcaatggcgt tgtcctgcgt
    12901 ggacgggctg tagccgaaac cgtccagaac caccagcagc gtcggatttc ccatgggttt
    12961 tcggccaaac ctcctcgagt tgcggggttg gggtagcggc cgggagttta acagaatcca
    13021 tggctacgag aggttgaacc ggtgtgtata atcgcgcggt ttccagccac gtcggagccc
    13081 ggagcagagg gcgcagagat ggatcagcta tttgagttca tcgttaatca tcccgttctc
    13141 gtgggggctt tcgtggtgct gctggtgttc ttcgtgcgca acgagaccca gcgcggcggc
    13201 cggtccgtct cggctcagga gctggtcgac ctggtcaacc gggaggacgc cgtggtgctc
    13261 gacgtgcggg acaagaagga tttcgaccag ggccacattc cccacgcgct caacatcccc
    13321 tacgcgtccc tggacggccg aatcgccgag ctgaacgctc acaaggaaaa gccgatcgtg
    13381 atcgcctgca agatgggtca gcatgccggt gctgcgggca cggcgctgcg caagcagggg
    13441 ttcgcgaacg tcacacggct caaaggaggc atcgccgaat ggcggaatca gaacctgccg
    13501 gtggtgaagg cgtagcccgg ggccagattc gtctggagcg cctgtacctc aaggacgctt
    13561 cgttcgaatc gccgcgctcg ccggcggtgt tcggcgaaag ctggaagccc gagatgcagc
    13621 tggacatcaa cacccgaacc aatccccagg aaggggatcg tttcgaggtg gtattgaccg
    13681 tgaccctgcg cgccaagctc gatgatgggc gcaccgccta catcatcgaa gtccagcagg
    13741 ccgggttgtt cgtgattcag ggcgctgaag acgccgtggt gcaccgggtg ctgggaacga
    13801 tgtgtccgaa cacgctgttt ccctacgtgc gggaaactct ggactcgctg gcggttcgcg
    13861 gcgggtttcc cgccattcag ctcgcgcccg tgaacttcga tgcgctttac gaggaagcgc
    13921 tcaagcagcg ggcacctccg gcaaaccccg tccagcactg atctaagttc cctcggcgta
    13981 gccgagctgt ctccacgcct cgtagaccac aacggcgacg gcgttggaga ggttcaggct
    14041 gcgcgagccc gcccgcatgg gaattcgcag cacgtgcgcc gctggaaacg tctcgagcac
    14101 gtccgccggc agcccccggg tctccggacc gaagagcagc acgtcgccgt cggtgaacgt
    14161 gatctccgag aagcgtctgg aaccgcgcgt gctcaccgcg tagcaccgtc cgcccccgca
    14221 ggcgtcgagc gcgtcgtcca ggctggcgtg aacgtcgacg caagcaaact cgtgatagtc
    14281 gagccccgcc cgccggagcc ttgagtcgtc catctcgaaa ccgagcggct cgaccaggtg
    14341 cagacgggcg ccggtgttcg cgcagacgcg gatgacgttg ccggtgtttg gcggaatctc
    14401 cggctggtac agcacgacgt gaaggggcat ggctcagagt tcggtcatcc cgagctcggc
    14461 aatcttccgg gtgagcgtgt tgcgtcccca gccgagcagc tcggccgcct cgcgcttgcg
    14521 cccgccggtg cgcctgagcg cggctcggat gaccacgctt tcgaaacgcg gcatcgccac
    14581 atccagcagc ggcagcccgc cgggctcgcg cagcaggtcg tcggcccagg cttccagcgc
    14641 ctgttcccag ctgtcgtttg tgtcctgctc gaccacatcg gagccgagct ccggcggcag
    14701 gtcggctaga tagatttcac gtccggaggc catcacggtg agccagcggc aggtgttctc
    14761 gagctggcgc acgttgcctg gccagctcag attcatcatg tactccatcg tgtcgggacg
    14821 gattatcttg ggctcgccgc ccagttcctc cgccgcctgc tccaggaaat ggctggccag
    14881 cgcgggaatg tccgttcgcc gttccgccag agtcggcacg tggacgctga tgacgttcag
    14941 ccggtgaaag agatcctccc ggaagctgcc gtccctcacc cgaacttcga ggtcctggtg
    15001 ggttgctgcg atgatgcgaa cgtccacctt gaccggggag tgtccgccca ctcgatagaa
    15061 ctccccgtcg gccagcacgc gcagcagccg ggtctgggtt tctgcgggca tgtcgccgat
    15121 ctcgtccagg aacagggtgc cgccgtcggc ctgctcgaaa cgcccttcgc gtcgcgagtt
    15181 ggcgcccgtg aaagcgccct tttcgtggcc gaacagctct gattccacca gctccctcgg
    15241 tatggccgcc atgttcaaag cgacgaattc gccctccgcg cgggggctgt ggcggtgcag
    15301 cgcacgggcg accagctcct ttcccgaccc cgatgcgccg cgtatcagga ccgtgacgtt
    15361 ggagtgtgcc agccggccga tggcgcggaa gacctcctgc atggcgggcg cctggccgat
    15421 gatttcctgg gcggtttcct gttcgggcgc cggcggcggc gcttcttcct gggcaaaggt
    15481 catggcccgt ttgacgatgg cgacggcttc gtccacgtcg aacggtttgg gcagatactc
    15541 gaacgcgccg ctctggtagg cgttgacggc gctgtcgagg tcggaatggg ccgtcatgat
    15601 gatgaccggc agctccgggt aggcctcgtg gatgcgacca atgaactcca tgccgtccat
    15661 gccgggcatt cgcacgtcgg taaggacggc gacgggccgc tcgtcctgca ggtgatgcat
    15721 ggcgtgatcg gccgaggcga aagcggcgat gggaatgtca gcctgctgca tggcccgctc
    15781 cagcacccag cgtatggacc ggtcgtcgtc gacgacccag actcgatttc tcacggctgc
    15841 gaattctcca gttgttcgat gtgctcgccg ttgctctcga cgggcaggaa taccgaaaag
    15901 cggg
//
LOCUS       DBNHLM010000008         6908 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1317944, whole genome shotgun sequence.
ACCESSION   DBNHLM010000008 DBNHLM010000000
VERSION     DBNHLM010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6908)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1317944"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="AC16QF_00335"
     CDS             complement(<1..279)
                     /locus_tag="AC16QF_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6447025.1"
                     /translation="MPTATEQTTPPLLQLDGVRVDLDGRSVLNDVSMSVAGGEVVVLL
                     GPNGAGKTTLLSLACGRRAPDAGRVRLAGHDPRTESSVRRQLGFVPQHV"
     gene            complement(333..626)
                     /locus_tag="AC16QF_00340"
     CDS             complement(333..626)
                     /locus_tag="AC16QF_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447026.1"
                     /translation="MEIVNHAREQLGPLTRQLLDAIDTESHPMEASFFAGLNVRLQHM
                     NDEGDLAELFFELSSTAFRGFAFSDHQAALIDRLLRAAEEIAFALSAPDDNAH"
     gene            900..1316
                     /locus_tag="AC16QF_00345"
     CDS             900..1316
                     /locus_tag="AC16QF_00345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6447027.1"
                     /translation="MPLRIGFQAPGGPLIAPLWFRYRDGSFWSCSPLSSALVKSLESH
                     PEVAFDVSTNEIPYKGVRGRALAECHPASGPDVLEDLLLRYLDGTDNPLAEWLLSRVD
                     GEALIRFHIEWLTSWDFSRRMSGLERIADRDATAEL"
     gene            1316..2710
                     /gene="phrB"
                     /locus_tag="AC16QF_00350"
     CDS             1316..2710
                     /gene="phrB"
                     /locus_tag="AC16QF_00350"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HIR6447028.1"
                     /translation="MRSIVWFRNDLRVTDNPALVEACARGEVTAVTCLCPAQLRAHDV
                     GPMRIAFLLRSLGVLSHDLASIGVSLKIIERPRFADLPEALVDFARQEGATHLEFNDE
                     YPLDERRRDAEVADACAAAGIVVGRHVADCTLSPGTVLTNSGSPYTVFSPFRRRWLER
                     VDRAQLTPMDAPRRRPGKGAGDPVPVTLDGVGAGLGEDLWPAGEAAAQKALKRFLSGP
                     VLDYGDTRDSPALDATSRLSPYLAIGAISVNQCLHAAVEANGGRLDGRSEGISKWITE
                     LIWREFYRHIVALFPHVSQGKSFRRELDALEWRDAPEDYEAWKAGETGYPLVDAAMRQ
                     LNETGWMHNRLRMVTAMFLTKHLLLDWRLGERYFMEKLVDGDFASNNGGWQWSASTGT
                     DAAPYFRILNPTTQGQRFDADGAFTRRMLPVLADVPDKWLFEPKRFDADLGYPDPIVE
                     HRAARARALALFKN"
     gene            complement(2729..3727)
                     /gene="pspF"
                     /locus_tag="AC16QF_00355"
     CDS             complement(2729..3727)
                     /gene="pspF"
                     /locus_tag="AC16QF_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HIR6447029.1"
                     /translation="MAAEQDRLLGESPAFLQILEEVSQAAPLEKPVLIVGERGTGKEL
                     IAARLHFLSNRWEGQFLKMNCAAISDSLLESELFGHEAGAFTGATRTHHGRFERAHGG
                     TLFLDELATTSALVQEKLLRVIEYGEFERVGGTKTLHCDVRLVAATNEDLPTRAEEGK
                     FRYDLLDRLAFDVLTLPPLREREEDILLLARHFAITMATELGREYFAGFGSAARDALM
                     THPWPGNVRELKNVVERCVYRSHPDEEVEEVVFDPFDSPYRPASQPSPPQGSTPAGNY
                     DLPLDLKQTVRELEIDLINRALENSKHNQRKAADLLKLTYHQLRGYLKKYELLKSD"
     gene            3934..4674
                     /gene="pspA"
                     /locus_tag="AC16QF_00360"
     CDS             3934..4674
                     /gene="pspA"
                     /locus_tag="AC16QF_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HIR6447030.1"
                     /translation="MSIFSRMSDIINSNINALLDKAEDPEKMVRLIIQEMEEALVEVR
                     STSARAIADKKELARRQEYLDADALEWERKAEVAVAKGRDDLAKGALVERNKAQEAAA
                     AITKDLQILDDTLAKLNDDVTALQAKIKDAKARQNAIIMRGKAAQTRLGVRRQLNDHN
                     IDDAIQRFESYERKMDDLEGQIEAYDMGRKTLADEIAELEVDETLDEELAQLKARMGN
                     ETRSSADAAGAKDAPGREQNCPEHSANG"
     gene            4683..4928
                     /gene="pspB"
                     /locus_tag="AC16QF_00365"
     CDS             4683..4928
                     /gene="pspB"
                     /locus_tag="AC16QF_00365"
                     /inference="COORDINATES: protein motif:HMM:NF006993.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HIR6447031.1"
                     /translation="MDGLAIAFFVPTVVFLTIVAPIWIFMHYRSKQRAQGALSEDERM
                     ELEALTSQAERMAERIETLESILDSETPGWRSRAQTE"
     gene            5038..5658
                     /locus_tag="AC16QF_00370"
     CDS             5038..5658
                     /locus_tag="AC16QF_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR6447032.1"
                     /translation="MEKLEAALHQLVDTARDQLSDRATAFIEETTATLEREFGRGTGP
                     DRPAAGRRRHGHGHRARDRATEDGIPSRSRRLYRDPGRAKIGGVCAGIARYYGIETWV
                     VRCAAVTGLIFVPQFVFPAYWIAYFLMGLPPREPASGSNGRARDDHSSPAPELGSKLS
                     PRRSLRNIQADLTQIELKLRRMETHVVSGQYELQKELNKIDEVGRG"
     gene            5694..5924
                     /locus_tag="AC16QF_00375"
     CDS             5694..5924
                     /locus_tag="AC16QF_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447033.1"
                     /translation="MDSVFMMVVLIVLISCGAGVANNYLRNQRKRAEADADPEVRTDL
                     DRLRERVEVLEKIVTDEKFHLAKEINRLEREP"
     gene            complement(5899..6597)
                     /locus_tag="AC16QF_00380"
     CDS             complement(5899..6597)
                     /locus_tag="AC16QF_00380"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR6447034.1"
                     /translation="MTFNRHPPLVIGHRGAAGLKPENTLASFSAALELGVDAVELDVH
                     LVEDALVVIHDSTLDRTTNGIGRLSDCTLDTLRLLDAGGGAQIPLLEEVFDLVPPDVG
                     INVELKGVGTGERLADFLTGRWRPEVLVSSFRRPELAAFRRRCPETNVAPLFSRWHPR
                     IWDVAARFTAWSVNLNIKLATRKRIADAQRRGFRVLVYTVNDLDLARSLVDAGVHGIF
                     TDHPERIRVPVLSD"
     gene            6653..>6908
                     /locus_tag="AC16QF_00385"
     CDS             6653..>6908
                     /locus_tag="AC16QF_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6447035.1"
                     /translation="MGYQVLARKFRPAGFDALVGQEHVVRALRHALDNDRLHHAYLFT
                     GTRGVGKTTIARLVAKSLNCEAGVSASPCGECSICREIAEN"
BASE COUNT         1263 a         2107 c         2300 g         1238 t
ORIGIN      
        1 gacgtgctgg ggcacgaacc cgagctgccg gcgcaccgaa ctttcggttc gcgggtcgtg
       61 gcccgccagc cgaacccggc cggcatccgg agcgcggcgg ccacaggcca gggagaggag
      121 ggtggtcttg cccgcgccgt ttggccccag cagcaccacg acctcgccgc ctgccacgct
      181 catcgaaacg tcgttcagca cgctgcgtcc gtccaagtcg acgcgtacac cgtccagctg
      241 cagaagggga ggcgtggtct gttcggtggc ggtgggcacg atgtgggcat catgccactg
      301 aacggtagca ggcgaaattg gggcccggcg ttctagtgag cgttgtcgtc cggtgcgctg
      361 agggcgaagg cgatctcttc ggcggcgcgc agcagccggt ctatcagggc ggcctggtgg
      421 tcggagaagg cgaaaccgcg aaacgccgtg ctggaaagct cgaaaaagag ctcagccaga
      481 tcaccctcgt cgttcatgtg ctgaagccgg acgttcaggc cagcgaaaaa cgaggcttcc
      541 atggggtgcg actcggtgtc gatggcgtcg agcagttgcc gcgtcagcgg cccgagttgc
      601 tcacgggcat ggttcacgat ttccatggtg cgatgctggc ggtggcgggg tgaaattgac
      661 gtcaaaacct cggtgagggc cgtggccgct ctgcgcttgc cggatgccgc cggccgcgcc
      721 ttgccggccg cgcctcgccg gccgcgcctc gccggccgcg cactttcacg gcttttttat
      781 cggaatctag ataacatcgg gttcttgcta cggagggtgg gttattgaat tttcagcctg
      841 agaaacacta tggggcctgg cgagggcgcg aggtggagcg atttttcgaa tctactcgcg
      901 tgcctctgcg cattggcttc caagcgcccg gcgggccgct gatcgcgccg ttgtggttcc
      961 gatatcggga tggttcgttc tggtcctgca gccccctgtc gtcggccctg gtcaagtcgc
     1021 tcgagagcca tccggaggtg gccttcgacg tatccacgaa cgagatcccc tataaaggag
     1081 tccgcggtcg ggcgttggcg gagtgtcacc cggctagcgg ccccgacgtc ctcgaggatc
     1141 ttctgctgag atatctcgac ggcacggaca acccgctggc ggaatggctg ctgtcgagag
     1201 tcgacgggga agcgctgatc cggtttcaca ttgaatggct gacgagctgg gacttcagca
     1261 gacgaatgag cgggctggaa cggatcgccg accgggacgc cacggccgaa ctttgatgcg
     1321 ctcgatcgtc tggtttcgca atgatcttcg cgtcacggac aacccggcgc tggtggaggc
     1381 gtgcgcgcgg ggggaggtca cggccgttac ctgtctctgt cccgcccagc tgcgggcgca
     1441 cgatgtgggc ccgatgcgca ttgccttcct gctgcggtcc ctcggcgtgc tctcgcatga
     1501 cctggcgtct atcggcgttt cgctcaaaat catcgaacgg ccccgctttg ccgacctgcc
     1561 ggaggcgctt gtagacttcg cgcggcaaga gggcgccacc cacctggagt tcaatgacga
     1621 gtatccgctg gacgagcgca gacgggacgc ggaggtcgcc gacgcgtgcg cggccgccgg
     1681 catcgtcgtt ggtcgccacg tggccgattg cacgctgtct ccaggaacgg tgctgacaaa
     1741 cagcggttcc ccctacacgg tgttcagccc cttccggcgc cgctggctgg aacgtgtgga
     1801 ccgggcccag ctgacgccga tggatgcgcc ccgccggcgc ccgggcaaag gcgccggcga
     1861 tcccgttccg gtaaccctgg acggcgttgg cgccgggttg ggtgaagacc tgtggccggc
     1921 cggggaagcg gcggctcaga aggcgctgaa gcgctttctc tccgggccgg tgctcgacta
     1981 cggagatacc cgtgattccc cggcgctcga tgccacgagc cgcctgtccc cctatctcgc
     2041 catcggggcg atatcggtca accagtgtct gcacgccgct gtcgaagcca acggggggcg
     2101 tctcgacggt aggtccgagg ggatatccaa gtggatcacc gagctcatct ggcgagagtt
     2161 ctatcgccac atcgtcgcgc tgtttcccca tgtctcccaa gggaagtcgt ttcggcgcga
     2221 gctcgacgct ctcgaatggc gggatgcccc ggaggattat gaggcctgga aagccggcga
     2281 gaccggctat ccgctcgtag acgctgccat gcgccagctc aacgaaaccg gctggatgca
     2341 caaccggttg cggatggtga ccgcgatgtt tctcaccaag cacctgctgc tggactggcg
     2401 actcggtgaa cggtacttca tggaaaagct ggtcgacggc gacttcgcct ccaacaacgg
     2461 cgggtggcag tggagtgcgt ccacgggcac ggatgccgct ccgtatttcc ggattctcaa
     2521 ccctacgacc cagggccagc ggttcgatgc tgatggcgcc ttcacccggc ggatgctgcc
     2581 ggtgctggcc gacgtgccgg acaagtggct tttcgagcca aaacgctttg acgccgacct
     2641 cggctacccg gatcccatcg tcgagcatcg cgcggcacgc gcgcgggctc tcgcgctttt
     2701 caaaaactga cccgcgccgc gcgatcagtc agtcggactt gaggagctcg tacttcttga
     2761 gatagccgcg aagctggtga tacgtgagct tgagaaggtc ggccgccttg cgctggttgt
     2821 gcttgctgtt ctccagcgcg cggttgatca ggtcgatctc cagctcccgc acggtctgtt
     2881 ttaggtccag tggcaggtcg tagtttcctg cgggcgttga gccctgcggc ggggagggct
     2941 gtgacgcggg ccggtagggt gagtcgaagg gatcgaaaac gacctcttcg acctcctcgt
     3001 cggggtggct gcggtatacg catcgttcca cgacgttctt aagctcccgg acgttgccgg
     3061 gccatggatg ggtcatcagg gcatcccgcg ctgcgctgcc aaagcccgcg aagtactcgc
     3121 ggcccagctc ggttgccatc gtgattgcga agtgcctggc aaggagcagg atgtcttcct
     3181 cgcgttcccg caggggggga agcgtgagga cgtcgaacgc cagccgatcg aggaggtcgt
     3241 agcggaactt gccttcctct gcgcgcgtgg gcagatcctc gttggtggcc gcgaccagcc
     3301 ggacgtcgca atgcagcgtc ttggtgccgc ctacccgttc gaactctccg tactcgatga
     3361 cgcgcagcag cttttcctga acgagggcgg aggtggtggc aagctcgtcg agaaacaggg
     3421 tgccgccgtg ggctcgctcg aaccggccgt gatgggtgcg ggttgctccg gtgaacgcgc
     3481 cggcctcgtg accgaaaagt tcgctttcca gcagcgagtc gctgatggcc gcacagttca
     3541 tcttgagaaa ctgtccctcc caacggttgg acagaaagtg cagccgcgcg gcgatgagtt
     3601 ccttgcccgt gccccgttct cccacgatca ggacgggttt ttccagcggc gctgcctgag
     3661 aaacctcctc gaggatctgc agaaaggccg gagattcccc gaggaggcga tcttgctctg
     3721 ctgccatgct gcgaatcttg ccaatattta gccgattctg ctacgtcctt ctagtgcccg
     3781 ggacattcgc gtcagaaata atatcaatta aaacatatag ttacgtatac gaaaggtttt
     3841 ggcacggaac ttgagaagag gtatgtgcaa cggcttcacg agattcagca cgtcgacggg
     3901 gccggcacag gaaactacag aggcagattt gagatgagca ttttttctcg catgtccgac
     3961 atcatcaatt cgaacatcaa cgccctgctc gacaaggcgg aagatcccga gaagatggtg
     4021 cggctcatca tccaggaaat ggaggaagcg ctcgtcgagg tgcgcagcac gtcggcccgg
     4081 gcaatcgcag acaagaagga gctggctcgc cgtcaggagt acctcgatgc ggatgccctc
     4141 gagtgggagc gcaaggccga ggtagcggtg gcgaagggcc gcgatgacct cgccaagggc
     4201 gcgctcgtag agcgcaacaa ggcacaggaa gcggcggcgg ccatcacaaa ggatctgcag
     4261 atcctcgacg acacgctggc aaagctgaac gacgacgtca ccgcgctgca ggcgaagatc
     4321 aaggacgcca aagctcggca gaacgcgatc atcatgcgcg gcaaggcggc gcagacgcgc
     4381 ctgggcgtgc gccgtcagct gaacgaccac aacatcgacg atgcgattca gcgattcgag
     4441 agctacgagc gtaagatgga cgatctcgag ggtcagatcg aggcctacga catgggtcga
     4501 aaaacccttg ccgatgagat cgccgagctc gaggtggacg agaccctcga tgaagagctc
     4561 gcccagctca aggcgcgcat gggaaatgaa acccgatcat ccgctgacgc cgcgggagcg
     4621 aaggacgcgc ccgggcggga acagaattgc ccggaacact ctgctaacgg ttaggagcaa
     4681 ccatggacgg cttagctatc gcgttcttcg tccccacggt cgtctttctc accatcgtcg
     4741 cgccgatctg gatcttcatg cattaccgga gcaagcagag ggcccagggg gccttgtccg
     4801 aggacgagcg catggagttg gaggctttga cgagccaggc ggagcgaatg gcggagcgta
     4861 tcgaaaccct cgaatcgatc ctcgattccg agacgcctgg gtggcggtcg cgggcgcaaa
     4921 cggaataggc tgagtggacg gagcgaaggc atgaacgatt cgaattcagg tggtggatcc
     4981 tctcgatcgc ctggcgcagg ccggcagcgc gacggagcct ctgattttca gcaggccgtg
     5041 gaaaagctgg aagcggcgtt gcaccagctg gtcgacacgg ccagggatca gctgtcagac
     5101 cgagccactg ccttcataga agaaaccacg gctacgctgg agcgagagtt cgggcgcggc
     5161 acagggcccg accggccggc ggccggaagg cgccgccacg ggcacgggca tcgggcgcgt
     5221 gatcgcgcaa cggaggacgg cattccctcg cgatcccgcc ggctgtaccg ggatccgggg
     5281 cgcgccaaga tcggcggcgt gtgcgccggc atcgcgcggt actacggcat cgagacctgg
     5341 gtggtgcgct gcgcggcggt gacggggctc atcttcgtcc cccaattcgt gttccccgcc
     5401 tactggatcg cctatttcct gatgggtctg ccgccgcgtg agcctgcgtc gggcagtaac
     5461 ggccgtgccc gcgacgatca ttcctcgccg gccccggagc ttggttcgaa gctgtcgccg
     5521 cgtcgcagcc tgcgcaacat ccaggcagat ctaacgcaga tcgagctcaa gctgcgccgc
     5581 atggaaaccc atgtggtttc cggccagtac gaactgcaga aagaactcaa caagatcgac
     5641 gaggtcggcc gtggctgacc cggccaccgc caaccctgat ccgggggcct gctatggata
     5701 gtgtcttcat gatggtggtg ctgatcgtgc tgatcagctg cggagcaggc gtggccaaca
     5761 actacctgag aaaccagcgc aagcgggcgg aggcagacgc cgaccctgag gtccgcacgg
     5821 acctagaccg tcttcgtgag cgggtcgagg tgctcgagaa gatcgtcaca gacgagaaat
     5881 ttcacctcgc caaggaaatc aatcgcttag aacgggaacc ctgatccgct cggggtgatc
     5941 ggtgaagatc ccgtggacgc ctgcgtccac caggctgcgc gcgagatcga ggtcgttgac
     6001 cgtgtagacg agcacccgaa aaccgcgtct ctgcgcgtct gcgatgcgct tgcgcgtcgc
     6061 cagcttgatg ttcaggttga ccgaccaagc tgtgaacctt gccgcgacat cccagattcg
     6121 cggatgccat cgactgaaca gcggcgccac gttcgtctca gggcaacgcc tgcgaaaggc
     6181 cgccagttcg gggcggcgga atgacgagac cagcacctcc ggtcgccacc ggccggtcag
     6241 aaagtcggcc agccgctcgc cggtccccac ccctttcagc tctacgttga taccgacgtc
     6301 tggcggcacc agatcgaaaa cttcctccag cagaggtatc tgcgcgccgc ccccggcgtc
     6361 gaggaggcgt agcgtgtcga gggtgcagtc ggagagccgg ccgataccgt tcgtggtgcg
     6421 atccagggtc gagtcgtgga tgaccaccag ggcgtcctca accagatgca cgtcgagttc
     6481 caccgcgtcc acgcccagct ccagcgcggc tgagaacgac gcaagcgtgt tttccggctt
     6541 caggccggcg gcgccgcggt gtccgatcac cagtggggga tgtcggttga aggtcacggg
     6601 cgggtccagg gcagtttggg tgtctgtggc ttacccacta caatgggcga tcatggggta
     6661 tcaggtacta gcacgcaaat tccggcctgc cggcttcgac gcgctcgtag gccaggagca
     6721 cgtcgtgcgc gccctgcgac acgccttgga caacgaccgt ctgcatcacg cctatctctt
     6781 taccgggact cgtggcgtgg gcaagacgac cattgcgcga ctggtggcca agagcctgaa
     6841 ctgcgaggcc ggcgtttccg catcaccctg cggcgagtgc tcgatctgcc gggagattgc
     6901 cgagaacc
//
LOCUS       DBNHLM010000009         4866 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_479960, whole genome shotgun sequence.
ACCESSION   DBNHLM010000009 DBNHLM010000000
VERSION     DBNHLM010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_479960"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="AC16QF_00390"
     CDS             complement(<1..735)
                     /locus_tag="AC16QF_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR6447036.1"
                     /translation="MRWLTFLRDGRATFGYVTSNGQGVVDVGARSETADLRSAIAAGA
                     LADLAGSCGDEADVPLDGLTYLPTIPNPDKILCVGLNYKAHQEETGRGGEGFPTIFVR
                     FATAQIGHEQPMIRPTESATLDYEGEIALVIGIPGRRISRSDALDHVAGVSIYNDGSV
                     REYQRQTSQFTAGKNFVGTGGFGPWMMTPDEVGDLNSMEITTRLNGEVMQNATADLLV
                     HGFAELIEYCSTFIELLPGDVIVTGTP"
     gene            complement(744..1826)
                     /locus_tag="AC16QF_00395"
     CDS             complement(744..1826)
                     /locus_tag="AC16QF_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HIR6447037.1"
                     /translation="MKAAVFREINQPMEIEEVALSKPGPREVLVRTAAAGVCHSDLHF
                     FNGTYPGLLPMVLGHESAGVVEAVGSDVHYVKPGDHVITCLSVFCGHCEFCLTGHLSL
                     CQEPETRRAPDQEPRISHDGAPVQQFANLGSFAEMMLVHEHAVAKIREDMPLDRAALI
                     GCGVTTGVGAVIHTAKVEPGSTVAVIGCGGVGLSCINGAAIAGASRIIAVDMVASKLD
                     LARKFGATDVVNAAEMDAINEVKALTGGGVHYSFEAIGLKATAEQAFGMLRNGGTATV
                     IGMIPPGDMVSLHGPDFLFEKKIQGSMMGSNRFRVDMPRFVDLYLQGKLHLDDLVSSR
                     IRLEDVNDAMAQLETGEIARNVIVFD"
     gene            complement(1898..2905)
                     /gene="pdxA"
                     /locus_tag="AC16QF_00400"
     CDS             complement(1898..2905)
                     /gene="pdxA"
                     /locus_tag="AC16QF_00400"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR6447038.1"
                     /translation="MTVGSDDDRPIAMTLGDPAGIGPEVAARLIAGGSLEGKLLLVGS
                     AWALGKGAEAAGVRLPDVEVVDDAGAIRGQVGLIDLGGEPESFRFGEVNAACGALAVR
                     AVEGAARLCLAGKVAAMVTCPINKAAVHAAGYTDDIGHQEILARLTGASWTATMLMTP
                     GLKVVHLSTHKSLIEAARFVTRRNVEEKLALLDETLGRWGLRNPRIAVAALNPHGGEQ
                     GLLGREELDEIEPAVAAARTAGIDAVGPLPADSVFNRAIDGEFDVVLAMYHDQGHIAI
                     KVHNFHESTTATLGIPFVRTSVDHGTAFDIAGSGRADERGLAAALDAARALSQGQLAR
                     L"
     gene            2979..3215
                     /locus_tag="AC16QF_00405"
     CDS             2979..3215
                     /locus_tag="AC16QF_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447039.1"
                     /translation="MLTLDDIARSFQVYAVCAPCQRTQPLPLAALIARFGGDLPVTAL
                     RARLRCETCGQRSADMRIVYVGPCGSAAGFHYRR"
     gene            complement(3227..4321)
                     /gene="ribB"
                     /locus_tag="AC16QF_00410"
     CDS             complement(3227..4321)
                     /gene="ribB"
                     /locus_tag="AC16QF_00410"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HIR6447040.1"
                     /translation="MSTQFDTIEDALAAIRSGEMVVVVDDDDRENEGDLIMAASKATS
                     EQVAFMIRHTSGILCAPILEDHAKQLHLDPMVARNDAPMSTAFTVSIDYRQGLTTGIS
                     AEERAATVQALTSSNVAAEDFVRPGHIFPLVARQGGVLVRSGHTEAGTDLAGLAGLPP
                     VGLLAELVNDDGTVQRLPELKEFAATHGLKIISIADLIAYRQMREQLVERTMEFAVHT
                     RIGEARAVAYKTKFEDAEHIALVFGEIGDEAVPVRIHREKLIDDIFGPQSNHPLGLLD
                     VALQRIQDLGGGVLIYLRSGFVGVPLEKLVDQSKEDERRAQWLEIGVGAQILRDLGLS
                     RIRLIAGREVDYVGVEGFGLVLESTELLND"
     gene            complement(4421..4636)
                     /locus_tag="AC16QF_00415"
     CDS             complement(4421..4636)
                     /locus_tag="AC16QF_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447041.1"
                     /translation="MSAYSIAREHLDVVASLAADNGVDLEAALRAVIGEAVERYRDLK
                     GADDLRAVLQYQLDNASGNEDYEFMRP"
     gene            complement(4669..>4866)
                     /locus_tag="AC16QF_00420"
     CDS             complement(4669..>4866)
                     /locus_tag="AC16QF_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447042.1"
                     /translation="GSLHWHEHPRLGPIVLFNSPLRFEGVDLPEVAEVPYVGAHNAAV
                     YSEFLGLDEDDIAELKQAEAI"
BASE COUNT          833 a         1653 c         1537 g          843 t
ORIGIN      
        1 cggcgtgccg gtgacgatca cgtccccggg cagaagctcg atgaaggtgg agcagtactc
       61 gatgagctcc gcgaacccgt gcacgagcag atcggccgtt gcgttctgca tgacctcccc
      121 gttcaggcgc gtggtgatct ccatgctgtt cagatcgccg acctcgtccg gtgtcatcat
      181 ccaggggccg aacccgcccg tcccgacaaa gttcttgcct gcggtgaact gggacgtctg
      241 acgctggtac tcgcgcacgc tgccatcatt gtagatgctg acccccgcca cgtgatcgag
      301 ggcatccgag cgggatatgc gtcgaccggg tattccgatc acgagggcga tctccccttc
      361 gtagtccagc gttgccgatt cggtcggacg gatcatcggc tgctcgtgcc cgatctgggc
      421 ggtcgcgaag cgcacgaaga tcgtggggaa tccttcgccg ccccgacccg tctcctcctg
      481 atgcgctttg tagttcagcc caacgcagag aatcttgtcc gggttcggga tcgtcggcag
      541 gtaggtcagc ccgtcgaggg ggacgtccgc ctcgtcgccg caggagccgg ccagatcggc
      601 cagggcaccg gccgcgatgg ccgatctcag atcggcggtt tcgctgcggg cgccgacatc
      661 gaccaccccc tggccgttcg aggtcacgta gccgaacgtg gcgcgcccat cgcggagaaa
      721 agtcagccag cgcatagttc cccctagtcg aagacgatca cgtttcgggc gatctcgccg
      781 gtctcgagct gggccatggc gtcgttgacg tcctccagcc ggatgcggct ggagaccaga
      841 tcgtccagat gcagcttgcc ctgcaggtag agatccacga accgcggcat gtccacccgg
      901 aaccggttgg accccatcat ggagccctgg atcttcttct cgaacagaaa atcgggccca
      961 tgcagggaga ccatgtcacc gggaggaatc atcccgatga cggtggccgt cccgccgttg
     1021 cgcagcatcc cgaatgcctg ctcggccgtt gccttcaggc cgatcgcttc aaaggagtag
     1081 tgcacgccgc ccccggtgag cgctttcacc tcgttgatgg catccatctc cgcggcgttg
     1141 accacgtcgg tcgcaccgaa cttgcgggcc agatccagct tggaggccac catgtccacc
     1201 gcgatgatcc ggctcgcgcc cgcgatggcc gcgccgttga tgcaggacaa tcccaccccg
     1261 ccgcagccga tgacggccac cgtggagccc ggctccactt tggcggtgtg aatgacggcc
     1321 ccgacaccgg tggtcacccc gcagccgatg agcgcggcac ggtccagcgg catgtcctcg
     1381 cggatcttcg ccacggcgtg ctcgtggacg agcatcatct ccgcgaacga gcccaggttg
     1441 gcgaactgct gcaccggcgc cccgtcgtgg ctgatgcgcg gttcctgatc cggagcgcgc
     1501 cgggtctcgg gctcctggca cagggacaga tgaccggtga ggcagaactc gcaatgcccg
     1561 cagaagaccg aaaggcaggt aatgacgtgg tcgccgggct tcacgtagtg aacgtccgag
     1621 cccaccgcct ccaccacacc ggcgctctcg tggcccagca ccatcggcaa gaggccggga
     1681 taggtcccgt tgaaaaagtg caggtcgctg tggcagacgc ccgccgcggc ggtgcgcacg
     1741 aggacctccc gcgggccggg tttggatagc gccacctcct cgatctccat gggctggttg
     1801 atctcgcgaa atactgccgc cttcatgctg cctcccctgc tgtgtgacgc ggacgcgacg
     1861 ccgcttcgat taagcctcgc gagtttgccc cacggcccta gagccgcgca agctgaccct
     1921 ggctcaacgc cctcgccgca tccagcgccg ccgccaggcc gcgctcgtcg gcccggcccg
     1981 agccggcaat gtcgaaggcc gttccgtgat ccaccgaggt tcgcacgaag gggatgccca
     2041 gggtcgccgt ggtgctctcg tgaaagttgt gcaccttgat cgcgatgtga ccctgatcgt
     2101 gatacatggc caggaccacg tcgaactccc cgtcgatggc ccggttgaac acggaatccg
     2161 ccggcagcgg ccccacggcg tcgataccgg cggttctggc cgccgccacg gcgggctcga
     2221 tctcgtcgag ctcctcgcgg cccagcagac cctgctcgcc gccgtgaggg ttgagcgctg
     2281 ccaccgcgat gcgcggatta cgcagccccc agcgccccag agtctcatcg agaagggcga
     2341 gcttctcctc cacgttgcgc cgggtaacga agcgggcggc ctcgatgaga gatttatggg
     2401 tggacagatg cacgaccttg agccccggcg tcatgagcat cgtggccgtc cacgatgccc
     2461 ccgtcagccg cgccagaatc tcctgatggc cgatgtcgtc cgtgtacccc gccgcgtgca
     2521 cggccgcctt gttgatcgga caggtcacca tggcggccac cttgccggcc agacagagcc
     2581 gggcggcgcc ttcgaccgcg cgcacggcaa gggcgccgca ggccgcgttg acctctccga
     2641 agcggaagga ttcgggttcg ccgccaagat cgatgagtcc gacctgaccc cggatggcgc
     2701 ccgcgtcgtc cacgacctcg acgtcgggga gcctcacgcc cgccgcttcg gcgcccttgc
     2761 caagcgccca ggcgctcccg accaggagga gctttccctc caacgacccg cccgcaatca
     2821 gcctggcggc aacctcggga ccgatacccg ccgggtcgcc gagggtcatg gcgattggcc
     2881 gatcatcatc agatcccacg gtcatggcat ccctccccgg ggaaaatact gtatatatat
     2941 acagtactca tggtcattag aacgtcgacg cccggaccgt gctcacgcta gatgacatcg
     3001 ctcgcagttt ccaggtctac gccgtgtgcg cgccgtgcca gcgcacgcag ccgctgccgt
     3061 tggcagcgct gatcgcccga ttcggcgggg acctgccggt gacggccctg cgagcgcggc
     3121 ttcgatgtga gacgtgcggg caacgaagcg cagacatgcg catcgtgtac gtcggcccct
     3181 gcgggtccgc cgccggcttc cactaccgcc gctgacgagg ggacggtcag tcgttgagaa
     3241 gctcggtgga ttcgagaacc aggccgaatc cttctacgcc cacgtagtcg acctccctgc
     3301 ccgcgatcag ccggatgcgc gacaggccca gatcacgcag gatctgcgcg cccacgccga
     3361 tctccagcca ctgagcccga cgctcgtcct ccttggattg atcgaccagc ttctcgagcg
     3421 gcaccccgac gaagcccgag cgcaggtaga tgagcacgcc gccgcccaga tcctggattc
     3481 gctgcaaagc gacgtccaga aggccgagcg ggtgattgct ctgcgggccg aagatgtcgt
     3541 cgatgagctt ttcccggtga atgcgaacgg gtacggcctc gtcgccgatc tcgccgaata
     3601 cgagcgcgat gtgctcggcg tcctcgaact tggtcttgta ggccacggcc ctggcctcgc
     3661 cgatgcgggt gtgcaccgcg aactccatcg tcctttccac cagctgctcg cgcatctgcc
     3721 ggtaggcgat gaggtcggcg atggaaatga tcttcaggcc gtgggtggcg gcgaactcct
     3781 tcagctccgg gaggcgctgc acggtgccgt cgtcgttgac cagctccgcc agcaggccca
     3841 cggggggcag gccggcgaga ccggccaggt ccgtgcccgc ctcggtatgc ccggaccgga
     3901 ccagaacgcc gccctggcga gccaccagcg ggaatatgtg gccgggacga acgaagtcct
     3961 cggccgccac gttgctcgag gtcagcgcct gaacggtggc cgcccgctcc tctgccgata
     4021 tgcccgtggt gaggccctgt cgatagtcga tggaaactgt gaaagccgtg ctcatgggcg
     4081 catcgttgcg cgccaccatg ggatccagat gcagctgctt ggcgtgatct tcgaggatgg
     4141 gcgcgcacag gataccgctg gtatggcgga tcatgaacgc gacctgctcc gacgtcgcct
     4201 tgctggcggc catgatcaga tcgccttcgt tttcccgatc gtcgtcgtcc accacaacca
     4261 ccatctcgcc cgagcggatg gcggcaaggg catcttcgat ggtgtcgaat tgcgtgctca
     4321 actgcgcttg accttaattc acaacgggtt cgaacgaccc ggggattgcc acgagcgcgc
     4381 tagattacca cgccacgggg aagggtatcg aatggcccgt tcaggggcgc atgaactcgt
     4441 agtcctcgtt gccggaagcg ttgtcgagct ggtattgcag aacggccctg agatcgtcgg
     4501 cgcctttgag gtcccggtaa cgctccaccg cctcgccgat gaccgcgcga agtgcagcct
     4561 cgagatccac gccgttgtcc gccgccagag aagctacgac atctaagtgc tcacgggcga
     4621 tgctgtaggc gctcaaacca acctccttac tttcaacgaa gctttcgatc agatcgcttc
     4681 ggcctgcttc agctcggcaa tgtcatcctc gtcgagaccg aggaattccg agtatacggc
     4741 agcgttgtga gcgcccacat aagggacttc cgcaacctcc ggcagatcca cgccttcgaa
     4801 ccgcagaggt gagttgaaaa ggacgatggg acccaggcgc ggatgttcgt gccagtgaag
     4861 ggagcc
//
LOCUS       DBNHLM010000010        10043 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1437993, whole genome shotgun sequence.
ACCESSION   DBNHLM010000010 DBNHLM010000000
VERSION     DBNHLM010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10043
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1437993"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="AC16QF_00425"
     CDS             <1..666
                     /locus_tag="AC16QF_00425"
                     /inference="COORDINATES: protein motif:HMM:NF037461.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HIR6447043.1"
                     /translation="SLQATVNRHDADYDPLFPYGFGLGAADVDTLGDELIEEDEALGL
                     AADESIGILTGRVVPPFEFFIGDSQAWKVPVRGRSSRSPTGAVSVTNVDHYSQEDARR
                     ISWDGSGGAQVYFQRSAGQDLSDHASRGARLRCKLRVDVRPQRRVELRMDSGYPQTAS
                     IDITAMLNELPLGTWEDVAFEMSAFAGGTDNLCAVDTPWLIWTDGVLGLSVSNLRIDL
                     GDG"
     gene            663..1994
                     /locus_tag="AC16QF_00430"
     CDS             663..1994
                     /locus_tag="AC16QF_00430"
                     /inference="COORDINATES: protein motif:HMM:NF014850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HIR6447044.1"
                     /translation="MTSILGRFPAVLVLLSLADAGLVPEAGAMPDEPVKVEVVRTARG
                     YALERGGRPYRIQGVGGLERLALLAESGGNSIRTWSVGTPEQSARELLDEAHRLGLTV
                     ALCLDIARERHGFDYDDDAAVAAQFERARADVLAHRNHPALLAWIIGNELNHDFKNPR
                     VYDAVNEISRMIHEIDGNHPTTTTIAGFSADMAEVISARAPDIDFLSIQSYAGVLDLQ
                     RRVRKAGYEGPYVLTEWGPPGHWEVATTSWGAPIEPDSSAKAKALRRAHATAIEPYPD
                     QLIGNYVFLWGQKQERTPTWYGLFTETGEKTESVDVMHYLWTGTWPGNRSPAVRSVRL
                     DGRRAGRDIVLVPGQTYPILARVRDPEGDALRYRWSVKSESRARQVGGDLEGAIADVD
                     GLIDEPSSSRAQLTAPEEPGAYRVFVYAYDGNGGAAHANVPFLVRSPAGAD"
     gene            2088..3314
                     /locus_tag="AC16QF_00435"
     CDS             2088..3314
                     /locus_tag="AC16QF_00435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6447045.1"
                     /translation="MIDFEIPAETKAIRAKAREFVQKECVAAEAQCTADNFDQVLSEL
                     RGKARAQGLWCPFIPEEHGGMGLKPLANALVQMELGESFLGALALNTQGPDDATMLTL
                     LEHGTDHQKEKFLKPLLDGQKRICYSMTEKAAGADATGMQTAAVLEGDNWVLNGEKWF
                     SSSADVADIAVVMAKTDPEAPRHERYSTFIVELPNPGYQILRNIDTMQPHTELGLKLG
                     HSHSEVKIENLVVPRENLLGGRGKGFAMGQHRLAYGRLRHGMHNVAMAQRALDLAAGH
                     VVNRETFGKRLADRQGVRWMLADCAAEIYKARLMLLHIAYKAERGMDLRQENGIAKVF
                     LAHMVHQVVDTALQLHGALGYSHDTPLASWYTSIRSQRLVDGPDEVHRWRTGANVIKA
                     YEEHGTTAPACGGELL"
     gene            3311..5191
                     /locus_tag="AC16QF_00440"
     CDS             3311..5191
                     /locus_tag="AC16QF_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012946.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6447046.1"
                     /translation="MSEARTEPAVRVDPATGVKRFATRAAKATDKIAGKGYSVIADEA
                     LTTLPPAPPGAVFDAEEQAKYRAFKEVRRGAADYMTMEGEFARYLEDVYSADPVPREA
                     LTDECEVLVVGAGFAGLLLWQKLSAAGFTDVRFCEKGGDVGGTWYWNRYPGIACDVES
                     YSYLPLLEEMGYIPSMKFASGFEILEYCQRMAEKFGFYDRCLFHTTVDRTEWDEAAGR
                     WTVYTNRGDAMRARFVVLANGILTTPQLARIDGMETFKGESFHTSRWNYHVDLAGKRV
                     GIIGTGATAVQVIPEIAKIVKELYVFQRTPSSIDVRDQRETTPEEYETWSHEPGWARA
                     RRARFSKISSGRTAIKANDDYLSGKVADFKERKQHERRLSAEEMINKQLETNFRIMEQ
                     IRARVDAIVEDPRTAEALKPYYPYGCKRPTFHDEYLPTFNLPHVTLVDTAPLGVTRIN
                     ERGVVHDGREYPLDVLIYATGFQWMATSTFNMIRGRGGRTLNEKWQEEGTRTFLGIHS
                     HGFPNLFIVSGPQGGGGSFNFTDAIDTHADYICWMLETMRDRGARTVDVRKEPEDEYA
                     EHCRAADVMTAPLRDCISYYNGHGDAEPGSLAYYGGGRWHKYRVRAQESLEPYDFDDI
                     PE"
     gene            5291..6787
                     /locus_tag="AC16QF_00445"
     CDS             5291..6787
                     /locus_tag="AC16QF_00445"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6447047.1"
                     /translation="MTDLVRKGWTVLALWALTMILGYFGLGGFLAETVEGIGGVPAWP
                     TALFVVLLAVSLWASVVWARGLKAKPEAGAALEERPLASGRRRFLTGTAAVTGGMLGA
                     AGATIGRVAPWATVTLPALRQPVTVTDPNPKDDWLGARVRSYRPLGDTGLMVSDIATG
                     STRIHHNSDPVGYLKSLLDRGVNYIDASPDYAPESEGIIAEAIVGRPRDQLVIASKFC
                     TRDGHLRQGSSVAAYMTAIDGTLSRMGTDYIDVAHIHSCDSTERLMDENVHEAFDRLK
                     DAGKVRHLGVSTHTPNLEEVADTAIDSGRFKVMMLAYHHGAWPKQMDIIDRAAAAGMG
                     IVAMKTLKGAKHRGLAEFRPEADSYAQAAFKWVLANPSVSNLVVSFYDNQHPDEYLFA
                     SGKSLERSDIAVLGKYDRLIAGTHCFQHCGDCLGACSSGLAINDILRHRMYFEDYGDE
                     KQAMQLYAKLDRQADVCIDCSAPCLGSCPQGVEIQERMIGAHEKLRWA"
     gene            complement(6819..8021)
                     /locus_tag="AC16QF_00450"
     CDS             complement(6819..8021)
                     /locus_tag="AC16QF_00450"
                     /inference="COORDINATES: protein motif:HMM:NF017984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR6447048.1"
                     /translation="MPTVTDISTRDARRLFLHRQGLLRREPFGRGGQAVARAVERLGW
                     VQIDTISVVERAHHHVLRSRVAGYQPRWLDDLQHRERAVFEYWSHAAAYLPMRAYRFY
                     LPMMRWSRRGDAADPKMAREVLRRVRDEGPLQSRDFEAAGHRSDGWWDWKPAKRTLEQ
                     LFHRGELMVTRREGFQKVYDLPERVLPESVDTSFPDASEWGRFVVGMMIDALGIASDK
                     EIAYQRIAAPRLYSRPILPLVQQAAADMVEAGELEQVTVEGGGSYLTRHLDWLPVRLG
                     RKRVTILSPFDNAVIMRERARELFGFDYAIECYLPAGKRRYGYFSLPILFGDELVGRM
                     DSKADRRTGTLTVNLLHLEPGADPAAIRDPLMQALNGFARDNGCTRVIMGATEPASLK
                     AELARVLG"
     gene            complement(8620..>10043)
                     /locus_tag="AC16QF_00455"
     CDS             complement(8620..>10043)
                     /locus_tag="AC16QF_00455"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006720 - isoprenoid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6447049.1"
                     /translation="APPSQEHPMAETTRPATEPTAESDYRFLSGNFAPVAEEVTAFDL
                     PVEGELPAELNGRYLRNGPNPFDDPDPGSYHWFTGAGMVHGIRLEDGRAEWYRNRYVG
                     SEQLAASRGEPDIPGANWNDNPSGPNTNVGGFAGTTWALVEAGGCPVELTYELETVAR
                     NDFFGTLPGAFTAHPKVDPQTGELHGMAYAWPQWMDHVQYVAVAPDGRVRRTVDVKLP
                     GMTMMHDFSLTQRYVVIYDQPVTVDFAMVGNTSFPMRWNPDYGNRLGLLPREGDADEI
                     IWIDAPLGYCFHPMNAYDDPDGSVIVDLCVYERMFDAYPRDPFGDSMASLQRWRVNPA
                     NRTISVDVIDASANEFPRHRGSLSTLPYRYGYCASPSSDMSAGWPTLKHDLQTGERSV
                     FDHGPGRAAGEPVFVAREGSAAEDDGYLITYVHDLAQSSAEFVVMDAQDFDRGYLARV
                     ALPQRVPFGFHGNWVSDRAVSPG"
BASE COUNT         1751 a         3263 c         3402 g         1627 t
ORIGIN      
        1 tcgctgcagg ccacggtgaa tcgccacgat gcggattacg acccgctgtt cccctacggc
       61 ttcgggctcg gtgccgcaga cgtcgatacc ctcggtgacg agctcatcga ggaagacgag
      121 gcgctcggcc ttgcggccga cgagagcatc ggcatcctca ccggccgcgt ggtgccgccg
      181 ttcgagttct tcattggcga cagccaggcg tggaaagtgc cggttcgcgg ccgctccagc
      241 cggtctccca cgggcgcggt ctcggtgacc aacgtcgacc actacagcca ggaggacgcg
      301 cgccgcatca gctgggacgg gagcggaggc gctcaggtct acttccagcg cagcgcgggc
      361 caggacctct ccgaccacgc ttcccgcggc gcccgactgc gctgcaagct gcgggtggac
      421 gtgcggcccc agcgcagggt cgagctcagg atggactccg gttatcctca gacggcgagc
      481 atcgacatca cggccatgct caacgagctg cccctgggga cctgggaaga cgtcgctttc
      541 gagatgagcg cgtttgccgg cgggacggac aacctatgcg cggtggatac cccgtggttg
      601 atctggaccg acggcgtgct gggtctttcg gtgtcgaacc ttcggatcga tctgggagac
      661 ggatgacttc aattctcggg cgctttccag ccgtgctcgt tttgctgtcc ctggccgatg
      721 ccgggctcgt gccggaagcg ggcgcgatgc cagatgagcc ggtcaaagtc gaggtggtgc
      781 gaacggcgag gggctacgcg ctcgagcgcg gtggccggcc ttaccggatc cagggcgtcg
      841 gcggtctgga gcgccttgcg ctgctcgccg agtccggcgg caactccatc cggacctggt
      901 cggttggaac tcccgagcag tccgcgcggg agctgctcga cgaagcgcac cgtctgggcc
      961 tcacggtcgc gctgtgcctg gacattgcca gggaacgtca cggcttcgac tacgacgatg
     1021 atgcggcggt ggccgcgcag ttcgagcgag cgagagcgga cgtgctggcg caccgcaatc
     1081 atccggccct gctggcctgg atcatcggca acgagctgaa ccacgacttc aagaaccccc
     1141 gggtctacga cgcggtgaac gagatttccc ggatgatcca cgagatcgac gggaatcatc
     1201 ccaccaccac gaccatcgcc ggcttcagcg cggacatggc tgaggtcatc tccgcccgcg
     1261 cgccggacat cgacttcctc agcatccagt cctacgcagg cgtcctcgac ctgcaacgac
     1321 gggttcgcaa ggccggatac gaggggccgt acgtgctcac tgagtgggga ccgcccggac
     1381 actgggaagt cgcgaccacc agctggggcg cgccgatcga gccggacagc tctgccaagg
     1441 ccaaggcgct gcgccgggcg cacgccacgg cgatcgagcc gtatccggac cagctcatcg
     1501 gcaactacgt ctttctgtgg ggccagaagc aggagcggac gccaacgtgg tacgggctgt
     1561 tcaccgaaac cggcgagaaa accgagtcgg tcgacgtcat gcactacctc tggacgggca
     1621 cctggcccgg caatcgcagc cccgcggtca gatcggttcg tctcgacggc cgcagggcgg
     1681 ggcgggacat cgtcctcgtt ccggggcaga cgtatcccat tctcgcccgg gtgcgcgatc
     1741 cggaagggga cgccctgcgc tatcgctgga gcgtcaagtc agagagccgg gccaggcagg
     1801 tcggaggaga cctcgagggg gcgatcgccg acgtggacgg gctgatcgac gaaccgtcgt
     1861 cgagccgcgc gcagctgacg gcccccgaag agcccggggc gtatcgcgtc ttcgtctacg
     1921 catacgacgg caatggcggc gcggcgcacg ccaacgtccc cttcctcgtc cgttctccgg
     1981 ctggagccga ctgaacgcac cacccctttc ggggcctgcc gtggttcccg cgcccgtgct
     2041 cctgatatag tcacgacaga cacgaacctg catagggaaa gggaccaatg atcgatttcg
     2101 agataccggc cgagaccaaa gccatccgcg ccaaggcgcg cgagttcgtg cagaaggaat
     2161 gcgtggcggc ggaggcgcaa tgcacggcgg ataatttcga ccaggtgctt tcggagctca
     2221 gaggcaaggc ccgcgcccag ggcctgtggt gtccgttcat ccccgaggag cacgggggca
     2281 tggggctgaa gccgctggcc aacgccctgg ttcagatgga gctcggcgag agctttcttg
     2341 gcgcgctcgc gctcaacacc cagggtccgg atgacgccac catgctcacg ctgctcgagc
     2401 acggcaccga ccaccagaaa gaaaagttct tgaagcccct gctggacggc cagaagcgca
     2461 tctgctactc gatgacggag aaggcggcgg gtgcggacgc cacgggcatg cagaccgcgg
     2521 ccgttctgga gggcgacaac tgggtgctca acggcgagaa gtggttcagc tcgtcggccg
     2581 acgtcgccga catcgccgtg gtgatggcca agacggaccc ggaggcgcca cgccacgagc
     2641 gctacagcac gttcatcgtc gagctgccca acccgggcta tcagatcctg cgcaacatcg
     2701 acaccatgca gccccacact gagctgggcc tcaagctcgg ccactctcac tccgaggtga
     2761 agatcgaaaa cctcgtggta ccgcgcgaga acctcctcgg cggccggggc aagggctttg
     2821 ccatgggcca gcaccggctt gcctacggcc ggctgcgtca cggcatgcac aacgtggcca
     2881 tggcgcagcg ggcgctggat ctggccgccg ggcacgtggt caatcgggag accttcggca
     2941 aacggctcgc cgataggcag ggcgttcgct ggatgctggc cgactgcgcg gcggagatct
     3001 acaaggcccg gctgatgctt ctgcacatcg cctacaaggc cgagcgaggc atggacttga
     3061 ggcaggagaa cgggatcgcc aaggtgttcc tcgcgcacat ggtgcatcag gtggtggaca
     3121 ccgcgctgca gctgcacggg gcgctgggct acagccacga cacgccgctg gcgagctggt
     3181 acacgagcat ccgcagccag cggctcgtgg acggcccgga cgaggtgcac cgctggcgca
     3241 cgggcgccaa cgtgatcaag gcctacgagg agcacggtac cacggcgccc gcctgcggcg
     3301 gcgagctgct gtgagcgagg cccggacaga gcccgccgtt cgcgtggatc ccgcgaccgg
     3361 ggtcaaacgc tttgccaccc gcgcggcaaa ggccacggac aagatcgcgg gcaagggcta
     3421 ttcggtgatt gccgacgagg cgttgacgac gctgccgccc gctccgccgg gcgcggtgtt
     3481 cgacgccgag gagcaggcga agtaccgggc cttcaaggag gtccgtcgcg gggccgccga
     3541 ctacatgacc atggaaggcg agttcgcgag gtacttagag gacgtctatt ccgcggatcc
     3601 ggtgccccgg gaagcgttga ccgacgagtg cgaggtgctc gtggtcgggg cgggtttcgc
     3661 cggtctgctc ctttggcaga agctttccgc agccggattc accgacgtgc gcttctgcga
     3721 gaagggcggc gacgtgggcg gcacctggta ctggaaccgc tacccgggca tcgcctgcga
     3781 cgtcgagtcc tacagctacc tcccgctgct ggaggagatg ggttacatcc ccagcatgaa
     3841 gttcgcctcg ggtttcgaga tcctcgagta ctgccagcgg atggcggaaa agttcggctt
     3901 ctacgatcgc tgcctgtttc acaccaccgt ggatcgcacg gagtgggatg aggcggccgg
     3961 tcgctggacg gtttacacga atcgcggcga cgccatgcgc gcgcgcttcg tggtgctggc
     4021 caacggcatc ctcaccacgc cccagctcgc gcgtatcgac ggtatggaga cgtttaaggg
     4081 cgagtccttc cacacatcac gctggaacta tcacgtggat ctggccggca aacgggtggg
     4141 cattatcggc accggcgcca cggccgttca ggtgatcccc gagattgcca agatcgtgaa
     4201 ggagctctac gtctttcagc ggacgccgtc gtccatcgac gtgcgcgatc agcgggagac
     4261 cacgccggaa gagtacgaga cctggtcgca cgaacccggc tgggcccggg cgcggcgcgc
     4321 gcgattctcg aagatctcct ccgggcggac ggccatcaag gccaacgacg actacctgtc
     4381 gggaaaggtg gccgacttca aggagcgcaa gcagcacgag cgcaggctct ccgccgagga
     4441 gatgatcaac aagcagctcg agaccaactt ccgcatcatg gagcagatcc gcgcccgcgt
     4501 ggacgccatc gtcgaggacc ccaggacggc cgaggcgctg aaaccctact acccgtacgg
     4561 gtgcaagcgg ccgacgttcc atgacgagta cctgcccacc ttcaacctgc ctcacgtcac
     4621 cctggtggac accgcgccgc tgggggtcac caggatcaac gagcgtggcg tcgtgcacga
     4681 cggccgggag taccccctgg acgtgctgat ctacgccacc ggcttccagt ggatggcgac
     4741 gtccacgttc aacatgatcc gtggacgggg tgggcgcacg ctcaacgaga agtggcagga
     4801 ggaggggacc cgcacctttc tcgggataca cagtcatggc tttccgaacc tcttcatcgt
     4861 ctcgggtccc cagggcggcg gcggcagctt caacttcacc gatgccatcg acacccacgc
     4921 cgactacatc tgctggatgc tggagaccat gcgcgaccgg ggcgcccgta cggtagacgt
     4981 gcgcaaggaa ccggaagacg agtatgccga gcactgtcgg gcggcggacg tcatgacggc
     5041 ccccctgcgc gactgcattt cctactacaa cggtcacggg gacgcggagc ctggcagcct
     5101 tgcctattac ggcggcggcc gctggcacaa gtatcgcgtt cgcgctcagg agagtctgga
     5161 accctacgat ttcgacgata ttccggagta gtggaaaccc ctggggatcg gcgagtactg
     5221 caacggacgc ggtttgatcc ccccgcggct gtcgatatag ttcagggata gccggcctgg
     5281 agaagggatg atgaccgatc tcgttcgcaa aggatggacc gttctggccc tttgggcctt
     5341 gaccatgatt ctcggctact tcggtctcgg cggctttctc gccgagacgg ttgaaggtat
     5401 cggtggggta cccgcttggc ccacggcgct attcgtggtc ctcttggccg tgagtctctg
     5461 ggccagcgtg gtgtgggcgc gcgggctgaa agcgaagccc gaagcggggg ccgcgctgga
     5521 agaaaggcca ctcgccagcg ggcgacgtcg ttttctcacg ggtaccgcgg cggtgaccgg
     5581 cggcatgctc ggggccgcgg gggcgaccat cggcagggtg gcaccctggg cgacggtgac
     5641 gcttcctgcc ctgcgccagc ccgtgaccgt cacggatccg aacccgaagg acgactggct
     5701 gggcgccagg gtgcgcagct accggccttt gggagacacc ggattgatgg tctccgacat
     5761 tgccaccggc tccacgcgca ttcaccacaa ctcggatccg gtcgggtacc tgaagagcct
     5821 tctggaccgc ggggtcaact acatcgacgc ctcacccgac tacgccccgg agagcgaggg
     5881 catcatcgcc gaggccattg tcgggcggcc ccgggaccag ctggtcatcg cctccaagtt
     5941 ctgcactcgc gacggccatc tccggcaggg ctcgagcgtc gcagcttaca tgaccgccat
     6001 cgacgggacg ctcagccgga tgggcacgga ctacatcgac gtcgcccaca tccattcctg
     6061 cgacagcacc gaaaggctca tggacgagaa cgtccacgag gctttcgatc gcctcaagga
     6121 tgccggcaag gtgcgccatc tgggagtgtc tacccacacg cccaacttag aggaggtcgc
     6181 cgacaccgcc atcgacagcg gccgtttcaa ggtgatgatg ctggcctacc accacggcgc
     6241 ctggccgaag cagatggaca tcatcgatcg cgcggccgcg gcgggcatgg gcatcgtcgc
     6301 catgaagacg ctcaagggcg ccaagcaccg cggccttgcg gaattcaggc ccgaagcgga
     6361 ctcctacgcc caggcggcct tcaagtgggt gctggccaac ccgtccgttt ccaacctggt
     6421 ggtttcgttt tacgacaacc agcatccgga cgagtacctg ttcgcctcgg gcaagtccct
     6481 cgagcggagc gacatcgcgg tgctcggcaa atacgaccgg ctcattgcgg gcacccactg
     6541 ctttcagcat tgcggtgatt gcctcggggc gtgttcgagt gggctcgcca tcaacgacat
     6601 cctgcgtcat cgcatgtact tcgaggatta cggggacgaa aagcaggcca tgcagcttta
     6661 cgccaagctg gaccggcagg cggacgtctg catcgactgc tcagcaccct gcctcggcag
     6721 ctgtccccag ggggtcgaga ttcaggagcg catgatcggc gcccacgaga agctgcgctg
     6781 ggcctgagac cttgtagccg attgggcctt cgccccggtc agccgaggac gcgagcaagc
     6841 tcggctttca gcgatgcggg ttcggttgcg cccatgatga cccgggtgca gccgttgtcc
     6901 cgggcaaacc cgttcagtgc ctgcatcaag gggtctcgga tcgcagcggg atccgcgccg
     6961 ggttcgagat ggagcaggtt caccgtcagg gtgcccgtgc gccgatccgc cttgctgtcc
     7021 atccgcccga cgagctcgtc gccaaacagg atcggcaggc tgaaatagcc atagcgccgc
     7081 ttgcccgcgg gcaggtagca ctcgatggcg tagtcgaagc cgaacagttc ccgggcacgc
     7141 tcccgcatga tcacggcgtt gtcgaacggc gagaggatcg tcacgcgttt gcggcccagc
     7201 cgcaccggca gccagtccag gtgacgcgtc aggtacgagc cgccgccctc gaccgtgacc
     7261 tgctccagct cccccgcttc caccatgtcg gcggccgcct gctgcactaa cggcaggatc
     7321 gggcgcgagt agagcctggg cgcggcaatg cgctgatagg cgatctcctt gtcgctggcg
     7381 atgcccaggg catcgatcat catgcccacc acgaagcggc cccactcgga agcatcggga
     7441 aacgacgtgt ccacgctctc gggcagcacc cgctcgggca gatcgtatac cttctgaaac
     7501 ccctcgcgcc gggtcaccat cagctcgccc cggtgaaaga gctgctcgag agtgcgcttc
     7561 gccggcttcc agtcccacca gccgtcgctg cgatgaccgg ccgcctcgaa gtcccgggac
     7621 tgcaggggac cctcgtcgcg cacgcgtcgc agcacttccc gggccatctt cggatccgcg
     7681 gcgtccccgc gccgcgacca gcgcatcatg ggcaggtaga agcggtaggc gcgcatgggc
     7741 agataggcgg ccgcgtggga ccagtactcg aacaccgccc gttctctgtg ctgcaggtcg
     7801 tcgagccacc tgggttgata cccggccacg cggctgcgca gcacgtggtg atgggcacgc
     7861 tccaccaccg agatggtgtc gatctgcacc cagcccaggc gctcgacggc gcgggccacg
     7921 gcctgacccc cgcggccgaa cggctcgcgc cgcagcagtc cctgccggtg caggaacagg
     7981 cggcgggcgt cgcgggtgga aatgtcggtg acggtcggca tgtgggctca ggcgcctgcg
     8041 ggtcttgcgt cgacgccgcg agcatagcgg ctcgctcgac ctgatggcca tggcggccgg
     8101 gtggatcgag gcgcatgttg cgctgaggac gacccgaaac gcgagagcct caccgccatc
     8161 gtgctcgggg cccgccatct cgggaatctc attcaggacg tgctggatct gtcgaggata
     8221 gaaagcgatc accgtgatcg atacggggat aggcgtccca gaggcattca gagacggcct
     8281 ctttgagcag tttgcgcggg ctgacgccac gatgaccaga cgctacggtg cgccggtccg
     8341 gggcttttca tcagccagcg gctggcttcg ctgctgggag gcagggtcga atacgcgccg
     8401 ggggagaccg gcagtgtttt ccgtctggtg ctggcgatct gcagggcgac gtccttcgcc
     8461 acaagatggg ttctgcagag atgaatccgt tcacggacat gctcccgaag cccatcgaca
     8521 ttcagcgatt gcagtcgctg ctgacgcgat tgctgagatc tcgcgacgcc gtcaaagctg
     8581 ccgatccctg accgggctcg aaatccccag gagctgccgt caaccgggcg atacggcgcg
     8641 atcgctcacc cagttgccgt gaaagccgaa gggcacccgc tgcggcagcg ccacccgggc
     8701 cagatagccg cgatcgaagt cctgggcgtc catcaccacg aactccgccg agctttgtgc
     8761 gagatcgtgg acgtaggtga tcaggtagcc gtcgtcctct gccgccgagc cctcccgggc
     8821 gacgaacacg ggctcgccgg ccgcccggcc gggcccgtga tcgaagacgc tgcgctcgcc
     8881 cgtttgcaga tcgtgcttga gcgtcggcca gccggcggac atgtcggaag acggcgatgc
     8941 gcagtagccg tagcgatagg gcagggtcga aaggctgccg cgatggcgcg gaaactcgtt
     9001 ggctgaggcg tctatgacgt cgacgctgat cgtccggttg gcgggattca cgcgccagcg
     9061 ctgaagagag gccatgctgt cgccgaaggg gtcgcgaggg tacgcgtcga acatacgctc
     9121 gtagacgcag aggtcgacga tcaccgagcc gtcggggtca tcgtaggcgt tcatcgggtg
     9181 gaagcagtag ccgagcggcg cgtcgatcca gatgatctcg tcggcgtcgc cttctctcgg
     9241 cagcaggccc aggcggttac cgtagtccgg attccagcgc atggggaagc tcgtgttgcc
     9301 caccatggcg aagtccacgg tcacgggctg gtcgtagatg acgacgtaac gctgggtgag
     9361 cgagaagtcg tgcatcatcg tcatgccggg cagcttaacg tccaccgtgc ggcggacccg
     9421 cccgtcgggc gccacggcga cgtactgcac gtggtccatc cactggggcc aggcgtaggc
     9481 catgccgtgc agctcgccgg tctgggggtc taccttcggg tgggccgtga acgcgccggg
     9541 cagggtaccg aagaagtcgt tgcgcgccac ggtttccagc tcgtaggtga gctccaccgg
     9601 gcagccgccg gcctcgacca gcgcccaggt ggtacccgcg aagccgccga cgttggtgtt
     9661 gggcccggag gggttgtcgt tccagttcgc tccggggatg tcgggctcgc cgcgagacgc
     9721 ggcaagctgc tcagagccca catagcggtt gcggtaccac tccgcgcggc cgtcctcaag
     9781 gcgaatgccg tgcaccatgc ccgcgccggt gaaccagtga tagctgcccg gatcggggtc
     9841 atcgaacggg ttcgggccgt tgcgcaggta gcggccgttg agctcggccg gcagctcccc
     9901 ctccacaggg agatcgaacg cggtgacctc ctcggccacc ggggcgaagt tcccgctgag
     9961 aaatcgatag tcgctttcgg ctgtgggttc cgttgcgggc cgggtcgttt ctgccatggg
    10021 gtgttcctgc gatggtgggg ccg
//
LOCUS       DBNHLM010000011        29878 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1617471, whole genome shotgun sequence.
ACCESSION   DBNHLM010000011 DBNHLM010000000
VERSION     DBNHLM010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 29878)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 29878)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29878
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1617471"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..446
                     /gene="yhbY"
                     /locus_tag="AC16QF_00460"
     CDS             144..446
                     /gene="yhbY"
                     /locus_tag="AC16QF_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HIR6447050.1"
                     /translation="MDAATRKRLKQIAHHLNPVVSVGGRGLSDELVAEIERALADHEL
                     IKIRIHSPDRSERRAVAEEIAGICEAEVVQTIGKVCVLFKVNPEPDKALSNLARHG"
     gene            complement(467..943)
                     /gene="greA"
                     /locus_tag="AC16QF_00465"
     CDS             complement(467..943)
                     /gene="greA"
                     /locus_tag="AC16QF_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR6447051.1"
                     /translation="MMPIPMTLEGAEALRKELSERKSVLRAQITQAIAEAREHGDLKE
                     NAEYHAAREQQGFNEGRIQEIENKLADAQVIDVSKITASGKVVFGATVTLIDTESGDE
                     VAYKIVGEDEADIKAGKLSVMSPIARAVIGKTVGDVSVVRTPGGEVEYEIDRVDLI"
     gene            complement(991..4224)
                     /gene="carB"
                     /locus_tag="AC16QF_00470"
     CDS             complement(991..4224)
                     /gene="carB"
                     /locus_tag="AC16QF_00470"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007243709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR6447052.1"
                     /translation="MPKRTDIDSILILGAGPIVIGQACEFDYSGAQACKALREEGFRV
                     ILVNSNPATIMTDPAMADATYVEPVEWATVARIIEQERPGALLPTMGGQTALNCALDL
                     VREGVLERFGVELIGASQDAIDKAEDRERFDKAMKRIGLETPRSAIAHSMEEAVQVQA
                     QLGFPCIIRPSFTLGGSGGGIAYNAEEFAEICARGLDLSPTNELLIDESLLGWKEFEM
                     EVVRDCVDNCIIVCSIENLDPMGVHTGDSITVAPAQTLTDKEYQLLRNASIAVLREIG
                     VDTGGSNVQFAIHPETGRLVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     LDNDITGVTPASFEPSIDYVVTKVPRFTFEKFDQADSRLTTQMKSVGEVMAIGRSFQE
                     SLQKALRGLETGMTGLDPLLEIGGEEAVARLRRELATPGAERIWYVADAFRFGLSLEE
                     VFELSAIDPWFLAEIEDLIAAESQLAGKRLDQIGADALYRLKRQGFSDARLGTLLGAP
                     EQQVREARHSMGIRPVYRRVDTCAAEFPSATAYMYSTYEEECEADPSERPKIMVLGGG
                     PNRIGQGIEFDYCCVHAALAMREDGFETIMVNCNPETVSTDYDTSDRLYFEPVTLEDV
                     LAIVDVEKPKGVIVQFGGQTPLKLVEALEQAGVPIIGTSAEAIDRAEDRQRFQALIRK
                     LGLKQPSNATAVDVETGLKGAREIGFPIVVRPSYVLGGRAMEIVFNEDELARYLLQAV
                     TVSNDSPVLLDRFLDQAVEVDVDAVSDGKQVVIGAIMQHIEQAGVHSGDSACALPPYQ
                     LSADLQDEIREQVRAMALELGVIGLMNVQLAVQEGEIFILEVNPRASRTVPFVSKCIG
                     VSLAKIAARCMAGKTLAEQGFTREIIPEQVNVKESVFPFTKFPGVDPILGPEMKSTGE
                     VMGVGDSFGEAFAKASLGAGVALPSGGRAFISVKDSDKPGLPALGRQLVDLGFQLVAT
                     RGTAKCFAEAGIPSEVINKVGQGRPHVADMLKNEQIDLIINTTEGHQSIRDSAVIRRL
                     ALQNKVCYTTTLTGGEAFCIAIRYSRDHQGETVRRLQDLHADMVGAS"
     gene            complement(4217..5344)
                     /gene="carA"
                     /locus_tag="AC16QF_00475"
     CDS             complement(4217..5344)
                     /gene="carA"
                     /locus_tag="AC16QF_00475"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR6447053.1"
                     /translation="MVRALLALEDGSVFKGLSIGVEGTSVGEVVFNTAMTGYQEILTD
                     PSYARQIITFTYPQIGNTGINPEDVESDRIWASGMVIRQTPKRASNWRMRQRLEDYLV
                     AEKVVAIADVDTRRLTRLIREKGAMAGCIAAGEAVSEAEAVALARDFPGLAGMDLAQV
                     VTRAEAGSWTQTDWSLESGYGEQTDARFHVVAYDFGVKRNILRLLAARGCRLTVVPAT
                     TPASSVLALEPDGVFLSNGPGDPEPCGYAIEAIGELLDRQIPIFGICLGHQLLGLASG
                     ATTVKMPHGHHGANHPVKDLRDGRVVITSQNHGFAVDADSLPDCLTATHVSLFDGTLQ
                     GIRRSDRPAFGFQGHPEASPGPQDCQYLFDEFIELMESRHA"
     gene            complement(5420..6235)
                     /gene="dapB"
                     /locus_tag="AC16QF_00480"
     CDS             complement(5420..6235)
                     /gene="dapB"
                     /locus_tag="AC16QF_00480"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR6447054.1"
                     /translation="MTRVAVTGAAGRMGKTLIQAIAEAEDLSLGAALERHDSTLLEAD
                     AGELAGVGRAGVAVGASLEDAAEAFDVLIDFSVPAATLAALEVCRERGRGMVIGTTGF
                     DADGLGRIQAAAETIPIMMAPNMSVGVNLCFKLMEIAASVLGDDVDIEVIEAHHRDKI
                     DAPSGTAVRMGEIIAVSLGRDLDEVAVYGRQGITGPRARSTIGFETIRAGDIVGEHTV
                     LFAGNGERIEITHRAQSRMNFAQGALRGVRFLAGRTSGLFDMQDVLGFKGALG"
     gene            complement(6290..7417)
                     /gene="dnaJ"
                     /locus_tag="AC16QF_00485"
     CDS             complement(6290..7417)
                     /gene="dnaJ"
                     /locus_tag="AC16QF_00485"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR6447055.1"
                     /translation="MAKRDYYEVLGVGRDASEDDIKKAYRRLAMKFHPDRNPDDASAE
                     EKFKEASEAYEVLADAEKREAYNRFGHAGVEASVGGTGGFDGGSFSDIFSDVFGDIFG
                     GGARGRSSVQRGSDLRYGLDLTLEQAVAGDNVEIRVPVLSACEVCDGSGARPGTSAST
                     CPDCGGAGQIRVAQGFFSLQQTCPRCRGAGRVIQDPCGNCGGSGREEKRKTLSVRIPA
                     GVDTGDRIRLAGEGEAGFNGGPPGDLYVQVEVRDHPIFTREGKNLYCEVPISFVDAAL
                     GGELEVPTLTGRVKLKIPPETQTGKLFRLRGKGVTPVRGGSVGDLLCRVQVETPVRLS
                     DEQKRLLQDFKATLAKGGSQHSPKETSWFQGVKDFFEGLKP"
     gene            complement(7498..9423)
                     /gene="dnaK"
                     /locus_tag="AC16QF_00490"
     CDS             complement(7498..9423)
                     /gene="dnaK"
                     /locus_tag="AC16QF_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR6447056.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGNDAKVIENAEGDRTTPSIVAYTD
                     DGEILVGQSAKRQAVTNPTNTLFAVKRLIGRKFKDDVVQKDIKMVPYKISEAPNGDAW
                     IDVKGEQLAPPQVSAETLKKMKKTAEEYLGEPVTEAVITVPAYFDDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAYGMDKKRGDTKIVVYDLGGGTFDVSIIEIAEIDGEHQFEV
                     LSTNGDTFLGGEDFDLRVIDYLADEFQKDNGVDLHRDPLALQRLKEAAEKAKIELSSS
                     QQTEINLPYITADQSGPKHLVLKLTRAKLESLVEELVTRTIDPCRTALKDANLNASDI
                     DDVILVGGQTRMPLVQQKVKDFFGKEPRKDVNPDEAVAIGAAIQAAVLSGDVTDVLLL
                     DVTPLSLGIETLGGVMSTLIEKNTTIPTKKQQVFSTADDNQTAVTIHVLQGERKQASS
                     NKSLGRFDLTDIPPAPRGLPQIEVSFDIDANGILNVSAKDKATGKEQSIIIKASSGLS
                     DDEIDQMVRDAEAHSEEDAKFEEMVTLRNQADGLIHAARKALTDAGDKASDTEKEAIG
                     SAADALEESLKNGDKADIEAKMQALSEASANLAQKMYEEQAAEAQSGAAEAGDDGAAD
                     GDTVDAEFEEVNEKDDK"
     gene            complement(9558..10157)
                     /gene="grpE"
                     /locus_tag="AC16QF_00495"
     CDS             complement(9558..10157)
                     /gene="grpE"
                     /locus_tag="AC16QF_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR6447057.1"
                     /translation="MTDDKPDAADLDGGSEESHTEVVESDPLEQEAPPPSASEAEIDD
                     PLAEAERQVAGLKDQVLRAQAEMENVRRRAARDVENAHKYALERFTSDLLPVLDSLEK
                     AVEASGESAGEAAGAIKEGVELSLKLFLTTLEKAGVIQVDPHGEPFDPQLHEAMAMVP
                     NPDTEPNSVIEVMQKGYTLNGRLVRAAKVVVSAALSQDG"
     gene            complement(10256..11305)
                     /gene="hrcA"
                     /locus_tag="AC16QF_00500"
     CDS             complement(10256..11305)
                     /gene="hrcA"
                     /locus_tag="AC16QF_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR6447058.1"
                     /translation="MARKQVEDNVDDRAQLLFKLLVERYLADGSPVASKKLAAFPEVE
                     VSPATVRNIMAELEDRGLVRSPHTSAGKVPTNLGLRFFVDSLLAVQPLGEEQIHRLEL
                     ELNPDLSPQELVESASNLLSHLTRMTCVVTLPRRDQVALRHVEFLRLSGDRVLVILVL
                     NDREVQNRVIHTDREYEDTELAQAANFINREFGGLSLVSIRDALLSSMQADKDGMDRL
                     MQTALDMAGRVFSESDQPLGKELVIAGESNLLDVSGDMATVRTLFDAFSRKGSILHLL
                     DRCLDSDGIQLFIGEESGYHILGDMSLVTAPYEVNGELAGVLGVVGPTRMAYQDVIPV
                     VDVTARVLGAAMNYG"
     gene            11402..13054
                     /gene="recN"
                     /locus_tag="AC16QF_00505"
     CDS             11402..13054
                     /gene="recN"
                     /locus_tag="AC16QF_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR6447059.1"
                     /translation="MLRELFVRDFALVESLEIGLCEGLTVITGESGAGKSILLGALGL
                     VLGDRADTDTIRPGAKRADVTAEFDLSGQPAAQAFLAEHDLEDADHPERCLVRRVVNQ
                     EGRSRAFINGAAVTRALLREFAEDLVDIHGQHEHQRLAEPATQLKLLDDYGVDAALLE
                     SCRESYRFWQRAVAEADALEAALASKQDRASLLSYQLEELAALGLEPGEFETLESEHK
                     RLSQAQDLRETVGHGLAVLEEADVLGRVSGALANLDDEHPALQAARDLLSSADSLVTD
                     AVRELRRYDDSLDVDEGQLGLLEERLTSVVDLARKHRVQPDRLVEHMALLQAELDAIS
                     TDRGSLDALREAAAGHEAEFRSTAGDVSLQRHEAAVHFASAVSGCMQNLGIGGGELRL
                     AFKEGESERGLESVEYQVMTNPKYPEAPLARIASGGERARISLAIQVVAAEKSALPCL
                     VLDEADVGIGGTTADVLGRLLRSLARHTQVICVTHAPQVAALGNHHLRVTKSSDQDTR
                     IEPLSEDTRIDEVARMLAGSGVTDKSRDYARTLLDEAHQALH"
     gene            13106..13507
                     /locus_tag="AC16QF_00510"
     CDS             13106..13507
                     /locus_tag="AC16QF_00510"
                     /inference="COORDINATES: protein motif:HMM:NF016258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990063 - Bam protein complex [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HIR6447060.1"
                     /translation="MVRLLASTLVLALLLAAGCSFRAPKFSLPRVHKITVQQGNVITQ
                     DMIDQLKPGMTRSQVAYVMGEPVFRNSFDESRWDYVYTVEVPGIFQDERRVSLYFEND
                     LLAYFTGDYAPSAAAGAAGAATDTSTAAEQG"
     gene            complement(13512..13781)
                     /locus_tag="AC16QF_00515"
     CDS             complement(13512..13781)
                     /locus_tag="AC16QF_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HIR6447061.1"
                     /translation="MKVDVVYALPDRQTQVGVSLADGATVADALGAVADTEPFSALDL
                     AAVPVGVFGESVGRDRQLRDGDRVEIYRPVLVDPKEARRRRAASA"
     gene            complement(13778..15139)
                     /locus_tag="AC16QF_00520"
     CDS             complement(13778..15139)
                     /locus_tag="AC16QF_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR6447062.1"
                     /translation="MAPATESRHGMWSSRWLFVLAAAGSAVGLGNIWKFPYIAGENGG
                     GAFVIVYLVCIALVGVPIMMAEVMMGREGRQSPINTMRTLTEAHGRSPLWVLIGWMGV
                     LAGFLILSYYVVIAGWALHYIWKMADGTFAGATADVANQTFNDFLADPWALMFWQTVF
                     MAATVWIIARGVIKGLESAIRWFMPLLFVLLMVLLGYAVTSDGFSQGWDFMFDFNWSA
                     LDANAVLIAMGQAFFTLSLGMGAIMAYGAYLPSGTSITTTVATIAGLDTLVALAAGLA
                     IFPIVFANGLEPGQGPGLMFVTIPLAFGQMPLGLVFGTVFFVLVSFAAITSAISISEP
                     ALAYLVEQYNAKRSRVAITLGVVGWLLGVGTVLSFNHWADVHVVGELTYFEFVDYASQ
                     NVLLPLGGLFIALFAAYALPRSVIGEQLGITDGWRALTWKFFCGLVAPLGVLAVFLYN
                     FLA"
     gene            15234..15710
                     /gene="smpB"
                     /locus_tag="AC16QF_00525"
     CDS             15234..15710
                     /gene="smpB"
                     /locus_tag="AC16QF_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR6447063.1"
                     /translation="MNSKTGKPAPKTIALNKRAKRDYHFEQTFEAGLVLEGWEVKSLR
                     AGKAQLVDSYVLVRDGEAWLLGANISPLPSASTHVVADPQRTRKLLLHTKEIAQIFTA
                     TRQKGYACIATALYWKGNRVKCELALGKGKKLHDKRATERERDWNRQKQRIMKQAG"
     gene            complement(15707..16192)
                     /gene="ribH"
                     /locus_tag="AC16QF_00530"
     CDS             complement(15707..16192)
                     /gene="ribH"
                     /locus_tag="AC16QF_00530"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR6447064.1"
                     /translation="MAQTAIDISQLPMIENAGRVAILKSKWYPELVDSMARCCEGVLR
                     DRGIDRVEVHTLPGSLELPLAAADLLAADSGGDIDAIVCFGIILKGETLHFEMITNEC
                     MRGLGEVMQRYRRPVIVEILPVLEMAHAESRAADDEFNKGIEAAAAAIEMIAWRRAVG
                     A"
     gene            complement(16569..16766)
                     /locus_tag="AC16QF_00535"
     CDS             complement(16569..16766)
                     /locus_tag="AC16QF_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447065.1"
                     /translation="MSTKPRLAPAIPGSEADFASLMAQAPNSTALFFELYAEFWQNGV
                     VAEEIKEMTRMRNARITDCGY"
     gene            complement(16782..17279)
                     /locus_tag="AC16QF_00540"
     CDS             complement(16782..17279)
                     /locus_tag="AC16QF_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6447066.1"
                     /translation="MTWIDEVEGATAMERVLAQRPELLARFKAFYRSFWEDGLVSRRV
                     LELCRLRIAAIHDCAQEWGIRDAEVALTQPQMEALRSGDLSNFDPPERAALELAERVP
                     YQHHQLTDEEVVHAEGLLGGPGTVSLLTALAFFDVVCRLRLTLELDEESALLASPPLA
                     DGAMV"
     gene            17343..18038
                     /locus_tag="AC16QF_00545"
     CDS             17343..18038
                     /locus_tag="AC16QF_00545"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR6447067.1"
                     /translation="MKREAGPGAIRSSLEILAILALTPVGLVLGGLSGSAALSSITGV
                     ALSVGAATLFLRASGESWRSIGFGRAMPVKRFVGFTAAALLGSYVATTFLVTPVMKAL
                     GAPPLEIGLLTRLIEGDLVSYLIFLLPIAWGSAAFGEELIARGFILHRFSNLFGDGVA
                     IVAQAALFALAHAYQGPVGMANIFALALVFGMVYVRCGRNLWPVIVAHGLVDTVGITL
                     IYLGRADLLLGAS"
     gene            complement(18051..19742)
                     /locus_tag="AC16QF_00550"
     CDS             complement(18051..19742)
                     /locus_tag="AC16QF_00550"
                     /inference="COORDINATES: protein motif:HMM:NF006203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HIR6447068.1"
                     /translation="MQSESVASRYLSLLAERGISHLFVNAGTDFAPLVEAYARSGDAQ
                     GPALPAPVLCTHENLAVGMAHGAYLVSGRPQAVMFHVSVGTANAVCAVTNAARDNVPL
                     LVTAGRTPILEDGALGARDTHIHWAQEMFDQAGMLRELVKWDYELRDPRQVHQVVDRA
                     LSMATSHPRGPVYLSLPREVLAEQMPDADAGARRTAVPVAVEPESSAIEELAGRIAAA
                     RCPVIVTSASGCEPDSVAVLADLADRFAIGIAEMMPRYLNAPMDHELHLGTQMQSIFD
                     AADLIVFLECDVPWIAARAAPRPETFIVQAGTDPLYSRYPMRTHRSDLTLAATPTALL
                     TGLADALASRAGQVDPDRRARLVELALRGKAAIAKLKAAEAEREGTITRAGLSAVLGD
                     ALDDETVIFNEYWGVPELLGRRQPGTYFYLPPAGGLGWGLPAALGAKQAAPEKTVVAA
                     VGDGAYLFANPAACHHAAAKHGLPVLTVIANNASWGAVDFATRAVYPDGSAVQSGERR
                     LSDLSPAPAFEAYCEASGGFGERVTEPSALAPAVERALKAVRDEGRQALLNVACV"
     gene            19854..21002
                     /locus_tag="AC16QF_00555"
     CDS             19854..21002
                     /locus_tag="AC16QF_00555"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6447069.1"
                     /translation="MHVQPIISEIARQADAADRSRTVDPDVIAAIKRSDVMGLSASAG
                     LGGLDAPVTEIARELGAVAPACSSTAWCLWNHLSTFHLFAGLLGPEHADFLKDIVERH
                     EWVCFPAGASTAVRAEEHDGEFSLRGTAAFGSGARYAEWSGVSFVPAEGGPPEFSMVD
                     LRSEGVRIDPTWEAMSLRASATDHVHYEGVRVPAERRVPFHFRYREVFRNPDHAMIDA
                     RYREDWVALSDLWLGAMAVGVAQACLDECCDGIRERVAILGTKMAERPTIHVNLGQAA
                     AAINAARDTVFAALSETDERLDSSVIPDEADYLRQVSASMQALQLCDESMRLMLRVLG
                     GNGLREGSNFERRYRDFQAMPLHINAHRDRITEQVGRHLLGLETENPF"
     gene            21027..22613
                     /locus_tag="AC16QF_00560"
     CDS             21027..22613
                     /locus_tag="AC16QF_00560"
                     /inference="COORDINATES: protein motif:HMM:NF015531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR6447070.1"
                     /translation="MTSNFPTTVTRRLASLLALAAIFAVLGGCGGGGGGSSPAVAVAP
                     EPEPEPEQWQPGVFEPASNFKDMCASPRIGAQFPDTQGTTTDENNWLRSWSNDLYLWY
                     EEIEDRDPARYTTPDYFDLLKTFASTPTGAPKDRFHFTYPTQEWEALSQSGQQPGYGA
                     AFVLLAAAPPRELAVAYIEPASPAEAAGLVRGARVLEIDGVSVVNGSDVDTLNAGLYP
                     SAAGEEHVFVVQEPGGGSRTVTLTSAIVTEDPVQFVKVIDSLTGPVGYLHFTRHIATA
                     ERELIDAIEFLGNAGVTDLILDVRYNGGGFLDIANELAFMIAGGGAASGRVFAEIQFN
                     DKHQTVNPVTGAVLEPEYFHTTTQGFSAEAGDPLPTLNLDRLYVLTTADTCSASESIV
                     NGLRGIDLDVVQVGSPTCGKPYGFYATDNCGTTYFSIQFKAVNAKGFGDYPDGFAPED
                     GAPAGAVTLPGCGVDDDFDNPLGDPAEALLATALEHRAGLGCPVGTATAARAEPRLAR
                     KGSGGEPLTPPRPYGMVLAR"
     gene            22615..23007
                     /locus_tag="AC16QF_00565"
     CDS             22615..23007
                     /locus_tag="AC16QF_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447071.1"
                     /translation="MRKICLIPLVLASACQSTTAEQLPAVLIGDRLEAIAEITEVIAD
                     ALRTEVSLSPSAFSESSLLTLEHAVGSDARGSAATGLLLERPEQFRLLWSDDGCSLTR
                     LRTGKTLPLDAAGCRPEAEATAGRAQSP"
     gene            23153..24127
                     /locus_tag="AC16QF_00570"
     CDS             23153..24127
                     /locus_tag="AC16QF_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6447072.1"
                     /translation="MNELPKEATDQTDRGVRDLITAVSRVSAWHDSAIEYEKVTGGIT
                     NENFKVRADGATFFVKIPGSGTEAFIDRANCHVANQIAAEVGIGPAVAHFFPDTGVEI
                     FDWLDGYRTMNWGDVYDNDVFLGTADLIRKFQTFEDLQMPLVQTAFEQTFEMIRMARE
                     MGTYLPPEMDRMEYLAHQIEAAVERGGIEYRPCHNDYYSMNILIHERTGELKLVDYEY
                     ASMNDECYDMGVYSGANYFTEEMDVMFIKRRYGGWDEERFARLKLYKLLADIKWSMWA
                     LVQDRIATVRFDYFNWFGTKMARLRGNSLDPRIDYWLNLVGRTGRLNA"
     gene            complement(24117..24407)
                     /locus_tag="AC16QF_00575"
     CDS             complement(24117..24407)
                     /locus_tag="AC16QF_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR6447073.1"
                     /translation="MTIDGVATDGAICFVDSEAGTLFLSGTESPHERSSSPPQGSAGG
                     GQPGLVLLHHMDVVPATPAAWSTNPLQAVVVDGYLHGRGVQMLIELVTAFTR"
     gene            complement(24462..26138)
                     /locus_tag="AC16QF_00580"
     CDS             complement(24462..26138)
                     /locus_tag="AC16QF_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HIR6447074.1"
                     /translation="MISDVTWMPGQKPFWWACCLIVCWLIQPAVADDDGRGRPYFKGS
                     PANPTALMRRMEADAHSAQALSAASSVGCVDGFADGFPCHNVHLQALVPLSTAGLSSA
                     SANDVWGWTDSISGREFALIGLNDGTGFVEITDPANPVVLGKLPTRNWNSTWRDIKTY
                     SDHAFIVSEADGHGMQVFDLTRLLDASPDITEAAHYDAFGSAHNIVINEDSGYAFAVG
                     TDTCSGGLHMVDISNPTNPRGAGCYSSAGYTHDAHCVVYQGPDLNYFDREICINSNED
                     TLVIVDVTDKAAPAVLSQVTYPGSGYTHQGWLTPDHSYFVFGDELDEQRFRHNTRTRV
                     FKLENLDQPAYAGSHDGTTPAIDHNQYVLGNHVYQANYRAGLRILELTDPSAAAFSEV
                     AYFDVYPSSNSASYNGAWSVYPYFPSGNVVVSGIEQGLFVLRPDLAGGPPPSGDPPAA
                     PIGLAVTDQGDGTALIEFTDESDNETAFEVDREMQHKRNGWRGFTTISLDADTTSYAD
                     VSGSGTFRYRVRAVNAFGASDPTAWVQVTVTTSGGAGDGGGTKCHPKRGC"
     gene            complement(26299..26656)
                     /gene="ssrA"
                     /locus_tag="AC16QF_00585"
     tmRNA           complement(26299..26656)
                     /gene="ssrA"
                     /locus_tag="AC16QF_00585"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            26777..28126
                     /locus_tag="AC16QF_00590"
     CDS             26777..28126
                     /locus_tag="AC16QF_00590"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6447075.1"
                     /translation="MLAAALAAALAAALAATPAAAQAPAQEPPLDSRSFIERSVTLLK
                     EEEYALARTYLAPALIDYRLTPGERSRAYYLRGFTFFAEGFYVSAGKDYARALEFNPD
                     NPVALAAMADLYFRGLGVEADYVIAYQLYSKAAADGHANAQFHTGYLELEGIGIEKNL
                     DSARGWLHKAADQDVVPAMIHMARSYRTEATDSPDPDQARAWYERAFAAGSADALVAL
                     AFMHQDGDFGAPDPEKALELFTRAAQEGSGAAQVSLAHMHITGELLPQDYAKALELLQ
                     QAARQGVPASYLGLGHIYDTGLGVKPDRATAENWYRLAAEAELLPAQLRLVRLLLRED
                     DSTSDREAMAWLARAARQDDPQAHNDYAWLLATHEEADLRDGDLAIEFAERAVARERI
                     PSYLDTLAAAYAEAGRFEDAVSTQEQAIAMLNEAEGEIRAELAEHLDAYQAGRPWRE"
     gene            28181..28426
                     /locus_tag="AC16QF_00595"
     CDS             28181..28426
                     /locus_tag="AC16QF_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447076.1"
                     /translation="MSIEQRRTKRIFRVIQEIVGAKGRDFRPGDIASVLRERGQPLGA
                     WEIRGQLSILEAEGLIRLVPESGSWEMASSENHRAVS"
     gene            28496..28756
                     /locus_tag="AC16QF_00600"
     CDS             28496..28756
                     /locus_tag="AC16QF_00600"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR6447077.1"
                     /translation="MAKTSETPDDLSDELDFESALEDLESLVESMESGEMSLEESLRA
                     FERGIKLTRHCQSALKAAELKVRMLTENDELVALDADDPPPE"
     gene            28753..29634
                     /locus_tag="AC16QF_00605"
     CDS             28753..29634
                     /locus_tag="AC16QF_00605"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR6447078.1"
                     /translation="MTPELDELRALINDALDHHLPSSSAIAQPLVDAMRYTTLGGGKR
                     IRPMLVCATCTGLGGALEDALAPACAVEFIHAYSLIHDDLPSMDDDELRHGLPSSHIK
                     FGEATAVLAGDALQALAFETLSRAPSGSDAQRLRAVQLLARAAGPQGMVGGQSYDLES
                     ERKKLSLSQLQTLHAAKTGALITAAVEIGALFGKPDLSPEKHVLISEFAARIGLAFQV
                     IDDILDVTATTEKLGKPAGSDTGAGKSTFPSLIGVESARGMAEELLAEALPMLDRVGI
                     KHSLLKDLAELIVKRAF"
     gene            complement(29643..>29878)
                     /locus_tag="AC16QF_00610"
     CDS             complement(29643..>29878)
                     /locus_tag="AC16QF_00610"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HIR6447079.1"
                     /translation="GIEFDESHQGTVQTAQGTADTHFVTLDEIVVGGITAHNVRAAII
                     EGEYPLEPLLGMSFLRQVSLQENAGVLTLTKQH"
BASE COUNT         5216 a         9966 c         9488 g         5208 t
ORIGIN      
        1 cacgccgggc atacggatcg gtccgctggc gctcaagcca acggccgctg gagccccccc
       61 gcttcttgga tccgcgcgta ctcatcgttt cactatgtca cacgttggcc ctagaatgcg
      121 cccccacccc gggggacacc gacatggacg ccgcaacgcg caagcgcctg aagcagattg
      181 ctcatcattt gaatccggtc gtcagcgttg gcgggcgcgg attgagcgac gagctcgtcg
      241 ccgagatcga gcgcgcgctg gcggatcacg agctcatcaa gatccgaatc cattcgcccg
      301 accgctccga gcggcgggcc gtggcagagg aaatcgcggg aatctgcgaa gccgaggtgg
      361 tgcagaccat cggcaaggtc tgcgtcctgt tcaaggtcaa tccggagccc gacaaggctc
      421 tgtcgaatct cgcgcgtcac ggctgatccg cacgggcgcg gcgatctcag atgaggtcaa
      481 cccggtcgat ctcgtactcg acctcgcctc ctggagtgcg aaccacgctt acgtccccga
      541 cggtcttgcc gatcaccgcc cgggcaatgg gtgacatcac ggaaagcttg cccgccttga
      601 tgtcggcttc gtcctcacca acgatcttgt aggccacctc gtcgccggac tcggtatcta
      661 tgagcgtgac cgtcgcgccg aagaccacct tgccgctggc ggtgatcttg gaaacgtcta
      721 tgacctgggc atccgccagc ttgttctcta tctcctggat tcgcccctcg ttgaagccct
      781 gctgctcgcg tgcggcgtgg tattcggcgt tttctttcag gtcgccgtgt tcgcgcgctt
      841 cggctatggc ctgcgtgatc tgcgcccgga gcaccgactt gcgctcgctg agctctttgc
      901 ggagcgcttc ggcgccctca agcgtcattg gaatcggcat gatgtccctg cccgaacaat
      961 cccgtttgtg cagcgtgtct gagcgtcggg ctaggatgcc ccgaccatgt cggcgtgcag
     1021 gtcctgcaac ctgcgaaccg tctccccctg atgatctctc gaatagcgga tggcgatgca
     1081 gaaagcctcg ccgcccgtga gggtagtggt gtagcacact ttgttctgca gtgcgagccg
     1141 acgaatgacc gccgagtcgc gaatggactg gtgcccctcc gtggtattga tgatcaggtc
     1201 gatctgctcg ttcttgagca tgtcggccac gtgagggcgg ccctggccga ccttattgat
     1261 gacctccgag ggaatgccgg cttcggcgaa gcacttcgcc gttccgcggg tggccaccag
     1321 ctggaacccg agatccacca gctggcggcc gagcgccggc aagccgggct tgtcggaatc
     1381 cttgacgctg atgaaagccc gcccgcccga gggcagggcg acgccggcac ctaaggaggc
     1441 cttggcgaac gcctcaccga agctgtcgcc aacgcccatg acctcgccgg tggatttcat
     1501 ctccgggccg agaatcgggt ccacccccgg gaacttcgta aacgggaaga cggattcctt
     1561 gacgttcacc tgttccggaa tgatctcccg ggtgaagccc tgttccgcca gcgtttttcc
     1621 cgccatgcag cgcgccgcga tcttggcaag agaaaccccg atgcacttgg agacgaaggg
     1681 gaccgtgcgc gaagcccgcg ggttgacctc gagaatgaag atctcgcctt cctggacggc
     1741 cagctgcacg ttcatcagcc cgatgacgcc cagctccagg gccatggccc gcacctgctc
     1801 gcggatctca tcctgcaggt cggcggaaag ctggtacggc ggcagcgcgc aggcggagtc
     1861 gccgctatgc acgcccgcct gctcgatgtg ctgcatgatc gcgccgatga cgacctgctt
     1921 cccgtcggag acggcatcca cgtccacctc cacggcctga tccagaaacc tgtccagcag
     1981 aacgggggaa tcgttggaca cggtgacggc ctggagcagg taacgggcca actcgtcctc
     2041 gttgaagacg atttccatgg cccggcctcc cagcacgtag gacggccgca cgacgatggg
     2101 gaacccgatt tcccgggcgc ccttgagccc cgtctcgacg tccaccgccg tggcgttgga
     2161 cggctgcttg aggccgagct tgcggatcag cgcctggaag cgctggcgat cctcggcacg
     2221 gtcgatggct tccgcgctcg tgccgatgat gggaacgccc gcctgctcaa gggcctccac
     2281 cagcttcagc ggcgtctggc cgccgaactg aacgatcacg cccttgggct tctcgacatc
     2341 aacgatggcc agcacgtcct ccagggtcac gggctcgaag tacagccggt ccgacgtgtc
     2401 gtagtccgtg gagacggtct cgggattgca attgaccatg atggtctcga agccgtcctc
     2461 gcgcatggcg agtgctgcgt gaacgcagca gtagtcgaac tcgatcccct ggccgattcg
     2521 gttcggtcct ccgccgagca ccatgatttt cggtcgctcg gacgggtccg cctcgcactc
     2581 ttcctcgtag gtcgaataca tgtaggcggt ggccgacggg aactcggccg cgcaggtatc
     2641 gacgcgccga tagacgggtc tgatgcccat gctatgccgc gcctcacgaa cctgctgctc
     2701 cggagccccc agcagcgttc ccagccgcgc gtccgaaaac ccctggcgtt tgagccgata
     2761 cagcgcgtcc gcgccgatct gatcgagccg cttgcccgca agctggcttt ccgcggcgat
     2821 gaggtcctcg atctcggcga gaaaccaggg atcgatggcc gacagctcga acacctcctc
     2881 cagcgagagc ccgaaacgga atgcgtcggc gacgtaccag atccgttctg cacccggggt
     2941 ggccagctcg cgccgcaacc gggcgacggc ttcctcaccg ccgatttcga ggagcgggtc
     3001 gagcccggtc atcccggtct ccaaaccccg cagcgccttc tgcagggact cctgaaagct
     3061 gcggccgatg gccatgacct caccgacgga cttcatctgc gtcgtgagcc gtgagtcagc
     3121 ctggtcgaac ttctcgaagg tgaaacgggg caccttggtg acgacgtaat cgatgcttgg
     3181 ctcgaaagag gcgggggtca cgcccgtgat gtcattgtcg agctcgtcca gcgtgtagcc
     3241 cactgccagc ttcgcggcga tcttggcgat ggggaagccg gtggccttgg aagccagcgc
     3301 cgacgaccgc gaaaccctcg ggttcatctc gatcaccacc aaccgaccgg tttccgggtg
     3361 gatggcgaac tgcacgttgg agccgccggt atccaccccg atttcccgca gaacggcgat
     3421 ggaggcgttg cgcagcagct gatattcctt gtccgtgagc gtctgcgccg gagccaccgt
     3481 aatgctgtcc ccggtgtgca cgcccatggg gtccagattt tcgattgagc agacgatgat
     3541 gcagttgtcc acgcaatcgc ggaccacctc catctcgaac tccttccagc ccagcaggct
     3601 ctcgtcgatg agcagctcgt tggtgggcga caggtcgagc ccccgggcgc agatctccgc
     3661 gaactcctcc gcgttgtagg cgatgccgcc gccgctgccg cccaaagtga acgacgggcg
     3721 gatgatgcac ggaaaaccga gctgggcctg gacctgaacg gcttcctcca tgctgtgcgc
     3781 gatggcagat cggggtgtct ccaggccaat gcgcttcatg gccttgtcga acctttcccg
     3841 atcctcggcc ttgtcgatgg cgtcctggct ggcaccgatc agctcgacgc cgaaacgctc
     3901 gagcacgcct tccctgacca ggtccagcgc gcaattgagc gccgtctgcc cgcccatggt
     3961 cggcagcagc gcacccggcc tctcctgctc gatgatgcgc gccacggtgg cccactccac
     4021 cggctcgacg tacgtcgcgt cggccatggc gggatccgtc atgatggtgg cgggattgga
     4081 gttgacgaga atcacccgga agccttcctc gcgcagcgct ttgcaggcct gagcgccgga
     4141 gtagtcgaac tcgcaggcct ggccgatcac gatgggcccc gcgccgagaa tgaggatgga
     4201 atcgatatcg gtacgcttag gcatggcgcg attccatgag ctcgatgaac tcgtcgaaca
     4261 gatactggca atcctggggc ccggggcttg cctccggatg cccctggaaa ccgaaggccg
     4321 gccggtcgga gcgacgaatg ccctgcagcg tcccgtcgaa tagagagaca tgggtggccg
     4381 tgaggcagtc gggcaggctg tcggcatcca ccgcgaaacc gtgattctgg ctggtgatga
     4441 cgacgcggcc atcgcggagg tccttgaccg ggtgattggc cccgtggtga ccgtggggca
     4501 tcttgacggt agtggccccg ctggccaggc ccagcagctg atgacccagg cagataccga
     4561 atatgggtat ctggcgatcg agcagctcgc cgatggcctc gatggcgtaa ccgcacggct
     4621 ccggatcgcc gggaccgttg gatagaaaga ccccgtccgg ctccagggca agaacgctgg
     4681 aggccggggt ggtggccggc acgacggtca ggcggcagcc gcgcgccgcc agcaggcgga
     4741 gaatgttccg cttgacgccg aagtcgtagg cgacgacgtg aaaacgcgcg tccgtctgct
     4801 caccgtaacc gctttcgagc gaccagtcgg tctgagtcca ggaaccggcc tcggccctgg
     4861 tcacgacctg cgccaggtcc atgccagcaa gcccgggaaa gtctcgggcc agcgccacgg
     4921 cctcggcttc gctgaccgcc tcgccggccg ctatgcatcc cgccatggcg cccttctccc
     4981 ggatgagccg ggtcagcctg cgggtgtcca cgtccgcgat ggcgaccacc ttctccgcga
     5041 ccaggtagtc ttccagacgc tgccgcatgc gccagttgct ggcccgcttc ggcgtctgcc
     5101 gaatgaccat gccgctggcc cagatccgat cggactccac gtcctccgga ttgatcccgg
     5161 tgttaccgat ctgcgggtag gtgaaggtga tgatctggcg ggcgtacgac ggatcggtga
     5221 ggatttcctg gtagcccgtc atggcggtat tgaaaacgac ttccccgacc gatgtacctt
     5281 ctacaccaat cgacagacct ttaaacacac tcccgtcctc gagagcgagc agtgcccgaa
     5341 ccaaatcgtt ttccaataac aggtcgcaaa aaggcgagat cgcccagaac cgatcccgcc
     5401 ttgaattcgg ggcggtagtc tacccaagtg cacccttgaa gcccagcaca tcctgcatgt
     5461 cgaagaggcc tgaggttcgg cctgcaagaa accggacgcc ccgcagcgcg ccctgggcaa
     5521 agttcatgcg gctctgggcg cggtgggtga tctcgattcg ctctccgttg cctgcaaaaa
     5581 gaaccgtgtg ctcgcccacg atgtcgccgg cccggatggt ctcgaaaccg atggtgctgc
     5641 gcgccctggg gcccgtgatc ccctggcgtc cgtacacggc tacctcgtcg agatcgcgcc
     5701 ccagcgacac ggcgatgatc tcccccatcc tgacggccgt gccggagggc gcgtcgatct
     5761 tgtcgcggtg gtgcgcctcg atcacctcga tgtccacatc gtcacccagc accgaggcgg
     5821 cgatctccat gagcttgaag cacagattca cccccacgct catgttgggc gccatcatga
     5881 tcgggatcgt ctccgcggcg gcctgaatcc ggccaagccc gtcggcatcg aatcccgtgg
     5941 tgccgatcac catgccgcgg ccgcgctcac gacagacctc cagcgccgcc agggtggctg
     6001 ccggaacgct gaagtcgatg agcacgtcga aggcttcggc ggcatcttca agcgaagccc
     6061 ccacggcgac gcccgcccgg cccacaccag ccagttctcc tgcatcggct tctaaaaggg
     6121 tggagtcatg tcgttccagc gcagcgccaa ggctcaggtc ttccgcctcg gcgatggcct
     6181 ggatcagtgt cttgcccatc cggcccgccg cacccgtcac ggcaacacgc gtcatcttcc
     6241 ggctccctag ttgctccggc cggtatagta ggcgatatct agggacggat cagggcttca
     6301 agccctcgaa aaaatccttt acgccctgaa accacgaagt ctctttgggc gaatgctggg
     6361 agcccccctt tgccagggtg gctttgaagt cctgcagaag ccgcttctgc tcatcggaga
     6421 ggcggacggg cgtctctacc tgcacacgac agagcaggtc gcccaccgat ccgccccgga
     6481 ccggggtcac gcccttgccg cgcagccgga acagcttgcc ggtctgggtt tcgggcggaa
     6541 ttttgagctt gacccggccg gtgagcgtcg gcacctccag ttctccgccg agggccgcgt
     6601 cgacgaagct gatcggcacc tcgcagtaca ggtttttgcc ctcgcgggtg aagatcgggt
     6661 ggtcgcggac ctcgacctgc acgtacaggt cgccaggcgg gccgccgttg aagcccgcct
     6721 caccctcgcc cgccagccgg atcctgtcac cggtatcgac cccggcgggg atccgtaccg
     6781 agagcgtctt gcgcttctcc tcccggccac tgccaccgca gttgccacag ggatcctgaa
     6841 tcacacgccc ggcaccgcgg catcgagggc acgtctgctg gagggagaag aatccctggg
     6901 ccacccgaat ctggcccgca ccgccacagt ccgggcaggt gctggcgctg gttcccggcc
     6961 gcgcgccgga gccgtcgcag acctcgcacg ccgagagcac gggcaccctg atctcgacgt
     7021 tgtcgccggc gaccgcctgt tccaacgtga gatcgaggcc gtagcggagg tctgaaccac
     7081 gctgcaccga gctgcggccg cgggcgccgc caccgaagat atcgccgaag acgtcgctga
     7141 agatgtcgct gaagctgccg ccgtcgaacc cgcccgtacc gccgacactg gcttcgacgc
     7201 ccgcatgacc gaagcggttg taggcctcgc gcttctcagc atccgcgagc acctcgtagg
     7261 cctcgctggc ctccttgaac ttctcttccg cggaggcgtc gtcagggttc cggtccgggt
     7321 gaaacttcat ggcgagtcgg cggtaggcct tcttgatgtc gtcttccgac gcatcacggc
     7381 cgacccccag aacctcgtag taatcccgtt tagccatctc gctcacacaa aaaggcgcac
     7441 gtctttcgcg tgcgccccca accgccgaaa gcagatcata atgcctgcga ccaacgttta
     7501 cttgtcgtcc ttctcattca cctcttcgaa ctcggcgtct accgtgtcgc catccgccgc
     7561 cccgtcgtcg cccgcctcgg cagcacccga ctgagcctcc gcggcctgct cctcgtacat
     7621 cttctgggcg aggttggcgg acgcctccga gagcgcctgc atcttggctt cgatgtcggc
     7681 cttgtcgccg ttcttcagag actcctccag ggcgtccgcc gcggatccga tggcctcctt
     7741 ctccgtatcc gaggccttgt ccccggcatc ggttagggcc ttgcgggcag cgtggatgag
     7801 cccgtccgcc tgattgcgca gggtgaccat ctcctcgaac ttggcgtcct cctcgctgtg
     7861 cgcctccgca tcgcggacca tctggtcgat ctcgtcatcg gaaaggccgc tggaggcctt
     7921 gatgatgatg gactgctcct tgcccgtcgc cttgtccttg gccgacacgt tcagtatgcc
     7981 gttggcgtcg atgtcgaagc tcacctcgat ctgaggcagg ccgcgtggcg cgggcgggat
     8041 gtcggtgagg tcgaaacggc cgagcgactt gttggagctc gcctgcttgc gctctccctg
     8101 caggacgtga atggtcacgg ccgtctggtt gtcgtcggcg gtggagaaca cctgctgctt
     8161 cttcgtggga atcgtggtgt ttttttcgat gagggtgctc atcaccccgc cgagggtctc
     8221 gatacccagc gacagagggg tcacgtcgag cagcagaacg tctgtgacgt cgccggagag
     8281 aacggcggcc tggatggccg cgccgatggc gaccgcctcg tccggattga cgtccttgcg
     8341 cggctcctta ccgaagaagt ccttgacctt ctgctggacc agcggcatgc gggtctggcc
     8401 gcccaccagg atgacgtcgt cgatatccga ggcgttcagg ttcgcgtcct tcagcgccgt
     8461 gcggcaggga tcgatggttc gagtcaccag ctcctctacc agggattcaa gcttggcacg
     8521 cgtcagcttt aggaccagat gcttgggacc ggactgatca gccgtgatgt aagggaggtt
     8581 gatctcggtc tgctggctcg aggagagctc gatcttggcc ttttccgccg cctctttcag
     8641 gcgctgcagc gcgagcggat ctctatgcag atccacgccg ttgtccttct ggaactcgtc
     8701 agccaggtaa tcgatcacac gcaggtcgaa gtcctcgcca cccagaaagg tgtcgccgtt
     8761 tgtggagagc acttcaaact gatgctcgcc gtcgatctcg gcaatctcga tgatggacac
     8821 gtcgaaagtt ccgccgccga ggtcgtagac cacgatcttg gtgtcgcccc gctttttgtc
     8881 catgccatag gcaagcgccg ccgcggtggg ctcgttgatg attcgcttca cgtccagacc
     8941 ggctatgcgg ccagcgtcct ttgtggcctg gcgctgtgag tcgtcgaagt aggcgggcac
     9001 ggtgatcacc gcttccgtga ccggctcccc gaggtactcc tcggccgtct tcttcatctt
     9061 cttgagcgtc tcggcggaca cctggggcgg tgcgagctgc tcgcccttga cgtcgatcca
     9121 ggcgtcaccg ttcggcgctt ctgagatctt gtagggcacc atcttgatgt ccttctggac
     9181 cacgtcgtcc ttgaacttgc gcccgatgag ccgcttgacg gcaaacagcg tgttggtggg
     9241 gttcgttacc gcctggcgct ttgcgctctg gccaacgagg atctccccat catcggtata
     9301 ggcgacgatg gacggcgtgg tacggtcgcc ctcggcattt tcgatgacct tggcatcgtt
     9361 gccttccagc accgccacgc atgagttggt ggtacccaag tcgataccga tgatcttacc
     9421 cattcttcat ttctccgatg tgtcggtccc gttgatccgg ctcgaagccg ttcacgtgac
     9481 cgtgaattca attcttcccg ctatatttgt ccgattggac ggacttcaac ccctgggtcg
     9541 agtacgggcg caatccgtca accgtcctga ctcagggccg cgctcacaac cactttggcg
     9601 gcccgaacca gcctgccgtt gagggtgtag cccttctgca tcacctcgat gaccgagttc
     9661 ggctccgtat ccggattcgg gaccatggcc atggcttcgt gcagctgcgg atcgaacggc
     9721 tcgccgtggg ggtcgacctg gatgacaccc gccttctcca gggtggtgag gaagagcttg
     9781 agagacagct cgacgccctc cttgatcgcc ccggccgcct cccccgcaga ctcccccgag
     9841 gcctcgacgg ccttctccag gctgtccagc acgggaagca gatcgctcgt gaaccgctcg
     9901 agggcgtact tgtgcgcgtt ttccacatcc cgcgccgccc ggcgcctgac gttctccatc
     9961 tcggcctggg ctcgcagcac ctgatccttg agtccggcaa cctgccgctc ggcctccgca
    10021 agcggatcat cgatctccgc ctcggacgcg ctaggcgggg gggcctcctg ttcgagcggg
    10081 tccgattcga ccacttcggt gtgcgactcc tcgctgccgc cgtccaaatc ggctgcgtcc
    10141 ggtttgtcgt ccgtcatgca aagcactcct tcgagccagc tttgtggaaa ctatcagcct
    10201 cgcggctgac gtcgcagatg tgggggcggc ggcgatctat ttcaagcccg ggcgctcacc
    10261 cgtagttcat ggcggcgccg agaacccgag cggtgacgtc caccacggga atgacatcct
    10321 ggtaggccat acgggttggg cccaccacac cgagcacccc ggcgagctcg ccgttcacct
    10381 cgtacggggc ggtgaccagg ctcatgtcgc ccaggatgtg gtaacccgac tcctcaccga
    10441 tgaagagctg tatcccgtct gaatccaggc acctgtccag caggtgcaga atgctccctt
    10501 tgcgggagaa ggcgtcaaac agcgtgcgca ccgtcgccat gtctccgctg acgtcgagca
    10561 gattgctctc tccggcaatc accagctcct tgcccaacgg ctggtcggat tccgagaaca
    10621 cgcgaccggc catgtccaga gccgtctgca tgagcctgtc catgccgtcc ttgtcggcct
    10681 gcatgctgga aagcagggcg tcgcggatac tcaccagcga caggccgccg aactcccgat
    10741 tgatgaagtt ggccgcctgg gcgagctccg tgtcctcgta ttcccggtcc gtgtggatta
    10801 cccggttctg aacctcgcgg tcgttgagaa cgaggatgac cagaacccgg tcaccagaca
    10861 ggcgcaggaa ctcgacgtgc ctgagggcaa cctgatcgcg gcggggcagc gtcaccacgc
    10921 aggtcatgcg ggtaaggtgg gagagcaggt tcgacgccga ctcgaccagc tcctggggag
    10981 aaagatccgg attgagttcc agctcgagcc ggtggatctg ctcctcgccc aaaggctgca
    11041 cggccagcag gctgtccacg aaaaagcgca accccagatt cgtgggcacc ttccccgccg
    11101 acgtgtgtgg cgaccggacc agaccgcgat cctctagctc ggccatgatg ttgcgcaccg
    11161 tagccgggct cacctccacc tcggggaagg cggcgagctt cttcgaggca accgggctgc
    11221 cgtccgccag atagcgctcc acgagcagct tgaagagaag ctgcgcacgg tcgtctacgt
    11281 tgtcttctac ttgctttctg gccattttcg ttggctaatg attacggtgc ggcgctctga
    11341 aggcccacgg tacaggcgtt ctgacagtcc actatgaacc taattcatcc cgcgtcaagc
    11401 catgcttaga gagctcttcg taagggattt tgctctggtg gagtcgctgg agatcggtct
    11461 ttgcgagggg ctgaccgtga tcaccggcga gtcgggagcg ggcaagtcca tactgctcgg
    11521 cgccctgggg ctggttctag gcgatcgggc agacacggac accatcaggc ccggcgccaa
    11581 gcgggcggat gtcaccgccg agttcgacct cagcggccag cccgccgctc aggcgtttct
    11641 tgccgagcac gacctggagg acgccgacca tcccgaaaga tgcctggtcc gtcgggtggt
    11701 caaccaggag ggtcgctcca gagccttcat caacggcgcc gcggtaaccc gcgctctgct
    11761 gcgcgagttc gccgaggacc tcgtggacat tcacggtcag cacgagcacc agcgcctggc
    11821 cgagccggcc acccagctca agctcttgga cgattacggc gtggacgccg cgctcctgga
    11881 gtcgtgccgg gagagctatc gcttctggca gcgggcagtg gcagaagcgg acgccctcga
    11941 ggccgccctg gcaagcaagc aggaccgcgc ctcactgctc tcttaccagc tcgaggagct
    12001 ggccgcgctg gggctagagc caggggagtt cgaaaccctg gaaagcgagc acaaaaggct
    12061 ctcccaggcc caggacctgc gcgagaccgt cggccacggc ctcgcggtgt tggaggaggc
    12121 cgacgtcctg ggccgcgtct cgggcgcgct ggccaacctg gatgacgagc atccggctct
    12181 tcaggctgca cgggatctgc tgtcatccgc cgacagcctc gtgaccgacg cggtacgcga
    12241 gctgcgccga tacgacgatt ccctggacgt ggatgagggc cagctcgggc tgctggaaga
    12301 gcggctgacg agcgttgtcg acctcgcccg caagcatcgg gttcagccgg accggctggt
    12361 cgagcacatg gccttactgc aggccgagct cgacgcgatc tccaccgacc gcggctccct
    12421 cgacgccctg cgcgaggcgg ccgccggaca cgaagccgag ttccgctcaa ccgccggcga
    12481 cgtgtctctc cagcggcacg aggcggccgt tcactttgcc tcggccgtca gcggttgcat
    12541 gcagaatctg ggtatcggcg gcggcgagct gcgcctggcc ttcaaagagg gcgagtccga
    12601 gcgtgggctg gagagcgtgg agtatcaggt gatgaccaac cccaaatacc cggaggcccc
    12661 gctcgcccgg atcgcctccg gcggcgagcg tgcccgcatc agcctcgcca tccaggtggt
    12721 ggccgctgag aagtcggccc tgccgtgcct ggtcctggac gaggcggacg tgggcatcgg
    12781 cggaaccacg gcggacgtgc tcggaaggct cttgagatct ctggcacggc acactcaggt
    12841 catctgcgtc acccacgccc cccaggtcgc cgccctgggc aatcatcacc tgcgggtgac
    12901 caaatccagc gatcaggaca cgcgcatcga gcccctttcc gaggacaccc gaatcgacga
    12961 ggtcgcgcgg atgctggcgg gttctggcgt caccgacaag tcgcgggact atgcccgcac
    13021 cctgctcgac gaagcccatc aggcccttca ttagcccgcg agatctgggt aacatccctg
    13081 gccttcgcgc aaacaccctg aggccatggt ccgactgctc gcttccacac tcgtcctcgc
    13141 gctgctcctt gcagccggct gcagcttccg agcgcccaag ttcagcctgc ctcgcgtgca
    13201 caagatcacc gtccagcagg gaaacgtaat cactcaggac atgatcgacc agctaaagcc
    13261 gggcatgacc cgcagccagg tcgcctacgt catgggggag ccggtcttcc gcaactcgtt
    13321 cgacgagagc cgctgggact acgtctacac ggtggaagtg cccggaatat tccaggatga
    13381 gcggcgggtg agcctgtact tcgaaaacga tctgttggcc tacttcaccg gcgactacgc
    13441 gccgtccgcc gccgccggcg cggccggagc cgcgaccgac acctccacgg cagccgagca
    13501 gggatgacca ttcaggcgga ggctgcccgc cgccgcctgg cttccttggg atcaaccagc
    13561 acaggccggt agatctcaac gcggtccccg tcccgcagct gcctgtcccg gccaacgctc
    13621 tccccgaata cccccacggg aaccgcggcc agatccagcg ccgagaaagg ctcggtatca
    13681 gcgacggccc caagggcgtc tgctaccgtg gcgccatccg ccaggctaac gcccacctgc
    13741 gtctgccggt ccggcagcgc gtagaccacg tcgaccttca tgcgaggaag ttgtagagaa
    13801 atacggccag cacccccagc ggcgccacca ggccgcagaa gaacttccac gtgagcgcgc
    13861 gccagccgtc cgtgatgccg agctgctcgc cgatgacgga cctgggcagg gcataggcgg
    13921 caaacagggc aatgaacagc ccgccgagcg gcagcaggac gttctgcgat gcgtagtcga
    13981 cgaactcgaa gtaggtcagc tcgccgacca catggacgtc tgcccaatgg ttgaaggaga
    14041 gcaccgtgcc cacgcccagc agccagccta cgacgcccag cgtgatcgcc acgcgggacc
    14101 gcttggcgtt gtactgctcc acgaggtagg caagggcggg ctcgctgatg gagatggcgg
    14161 aggtgatggc ggcaaagctc accagaacga agaacacggt accgaagacc aggcccagcg
    14221 gcatctggcc aaaggccagc gggatggtga cgaacatgag ccctgggccc tggccgggct
    14281 ccaggccgtt ggcgaacacg atgggaaaga tggccagccc ggccgccagg gcgaccaggg
    14341 tgtccaggcc cgcgatggtc gccacggtgg tggtgatgga cgtgccgctc ggcaggtacg
    14401 ccccgtaggc catgatggcg cccatcccga ggctcaaggt gaagaaggcc tggcccatgg
    14461 cgatcagcac cgcgttggcg tccagggcgg accagttgaa gtcgaacatg aagtcccagc
    14521 cctgggagaa cccgtcggag gtcaccgcgt aaccgagcag caccatcagc aggacgaaca
    14581 gcagcggcat gaaccaccgt atggccgact cgagaccttt gatcacgccg cgggcaatga
    14641 tccagaccgt ggccgccatg aagaccgtct gccagaacat gagcgcccag ggatcggcca
    14701 ggaagtcgtt gaatgtctgg ttggcaacgt cggcggtggc tccggcgaac gttccgtcgg
    14761 ccatcttcca gatgtagtgc agggcccatc cggcgatgac cacgtagtag ctgagaatga
    14821 gaaacccggc cagcacgccc atccagccga tgagcaccca gagcggcgag cggccatggg
    14881 cttcggtgag cgtgcgcatg gtgttgattg ggctctggcg gccttccctg cccatcatca
    14941 cctcggccat catgatgggc acgccgacga gggcgatgca gacgaggtag acgatgacga
    15001 acgcgccgcc gccgttctcc ccagctatgt aagggaactt ccagatgttc ccgaggccca
    15061 cggccgatcc cgctgctgcc agcacgaaca accatctgct ggaccacatg ccgtgccggg
    15121 attcggtagc cggggccatg agcacttcca aggcgatcgc gttgggccgg cattgtggcc
    15181 caacgtcttt ggggcctcaa ccgcatataa tccgaggcca tgaggtcctc gatttgaaca
    15241 gcaaaacggg caagcccgca ccaaaaacca tcgcgctgaa caagcgcgcg aagcgcgact
    15301 accacttcga gcagaccttc gaagccggcc tggtgctcga ggggtgggag gtgaaaagcc
    15361 tgcgcgcggg caaggcgcag ctggtggaca gctacgttct cgttcgcgac ggcgaggcgt
    15421 ggctcttggg cgccaacatc tcaccgctgc ccagcgcatc gacccatgtg gtcgcggatc
    15481 ctcagcgcac ccgcaagctg ctgctccaca cgaaagagat cgcgcagatc ttcacggcca
    15541 cccggcagaa gggctacgcg tgcatcgcca cagcgctgta ctggaagggc aaccgggtca
    15601 agtgcgagct agcgctgggc aagggcaaaa agctgcatga caagcgggcc accgagcgcg
    15661 agcgtgactg gaaccgccag aagcagcgga tcatgaagca ggcgggctaa gcgcctacgg
    15721 cccggcgcca ggcaatcatc tcgatggcgg ccgcagccgc ctcgatccct ttgttgaact
    15781 cgtcatcggc ggcccgtgat tccgcatggg ccatctccag tacgggcagg atctccacga
    15841 tgaccggccg ccggtatcgc tgcatgacct cgccgaggcc gcgcatgcac tcgttggtga
    15901 tcatctcgaa gtgcagggtt tcgcccttca ggatgatgcc gaaacagacg atcgcgtcga
    15961 tgtcgccgcc cgagtcggca gcaagcaggt cggcggcggc caggggcagc tccagggatc
    16021 ccggcagcgt gtgaacctcc acccggtcga tgccccggtc gcgaaggacc ccctcgcagc
    16081 agcgcgccat gctgtccacc agttcgggat accacttgga cttgagaatc gccacccgcc
    16141 cggcgttctc tatcatcggc agctggctga tgtctattgc ggtctgcgcc atgtcggcaa
    16201 gctctgtgtc agcggagggc gaagaatacc cgaatgagcc gacgcgggcc cgttcaggac
    16261 cccggtgtcg gcacgacggt caccggcatc tcctcgggct ccagcccgag catgatgagc
    16321 accttggaca tgccgagaaa gcagccgacg cccagcgcca tctcgacgat ctgcgcatcg
    16381 gagaagtgag ctttgagagc ggccttggca tccgcatcga gtgggccaga agccccgatg
    16441 atggcgtccg tcagcttcag ggccgccgtc tcggccggcg cgagcgagcg ctcgtacccg
    16501 tccgcgatct gctcgacctt cgcttcgtcc agccccgccg cacgggcgga gtcgaatcgt
    16561 acgttttttc agtagccgca gtcggtgatg cgcgcgttgc gcatgcgcgt catctccttg
    16621 atctcttcag cgacgacgcc gttctgccag aactcggcgt agagctcgaa aaaaagggcg
    16681 gtggagttgg gcgcttgagc catgagcgat gcgaaatcgg cttcactccc ggggattgcg
    16741 ggtgccagtc gaggcttggt ggacatcgga tgctcccctg atcagaccat ggcgccgtcg
    16801 gcaaggggcg ggctcgcgag caaagcgctc tcctcgtcca gctccagcgt gagcctgagc
    16861 ctgcagacga cgtcgaagaa agcgagcgcc gtcagcaggg acaccgtgcc cggcccaccc
    16921 aggagcccct ctgcatgcac aacctcttca tcagtcagct gatgatgctg ataaggcacg
    16981 cgctcggcca gttccaatgc cgcccgctca ggggggtcga agttggagag gtctccgctt
    17041 ctaagcgctt ccatctgcgg ctgcgtcagg gccacctcgg catcccggat cccccattcc
    17101 tgggcgcagt cgtgaatggc cgcgatgcgc agcctgcaca gctcgaggac ccgccgcgag
    17161 accagcccgt cttcccagaa gctccggtag aaggccttga aacgggccag cagctccggg
    17221 cgctgtgcca gcacccgctc catggccgtt gccccttcaa cctcgtcgat ccacgtcatg
    17281 ggtgcgcgcc tttccaaatg aggcaaaaat agcacttccc atgtaatcct tcgaggcgga
    17341 ccatgaagcg tgaagcaggg cccggcgcga tccgctcgtc cctcgaaatc ctcgccattc
    17401 tggcactgac ccccgtgggt ctggtgctgg gagggctgtc ggggtccgcg gcgctcagct
    17461 ccatcaccgg cgtggcgctg tccgtcgggg cagccaccct cttcctgcgc gcgagcggtg
    17521 agagctggcg cagcatcggc ttcggccgcg ccatgccggt caagcgcttc gtcggtttca
    17581 ccgccgccgc gctacttggc agctacgtgg ccaccacctt cctggtgacg ccggtcatga
    17641 aagccctcgg cgcgcccccg ctggagatcg gtctgctcac ccggctgatc gaaggcgacc
    17701 tggtgagcta tttgatcttc ctgctgccca tcgcctgggg aagcgccgcg ttcggtgagg
    17761 agctcatcgc gcggggcttc atccttcacc gcttttccaa cctgttcggc gacggggtcg
    17821 ccatcgtcgc ccaggcggcc ctgttcgccc tggcgcacgc gtatcagggg cccgtcggca
    17881 tggcgaacat ctttgccctg gcgctggtgt tcgggatggt ctacgtacgc tgcgggcgca
    17941 atctgtggcc cgtgatcgtc gctcatggcc tggtcgacac cgttggtatc accctgatct
    18001 acctgggccg ggccgacctc ctactggggg caagctgatc cgggccgccg tcagacacag
    18061 gccacgttca acagcgcctg ccgaccttcg tcccggaccg ccttcaacgc gcgctccacg
    18121 gcgggggcga gggccgaggg ctcggtgacc cgctcgccga aacccccgga ggcctcgcag
    18181 taggcttcga aggccggtgc aggggaaagg tctgaaaggc gtctttcgcc actttgcacc
    18241 gcgctgccgt ccgggtagac ggcgcgggta gcgaaatcga ccgcgcccca gctggcgttg
    18301 ttggcgatca ccgtgagaac ggggagaccg tgcttggccg cggcatggtg gcaggccgcc
    18361 gggttggcga agaggtacgc gccgtctccc accgcggcca ccaccgtctt ctccggcgcc
    18421 gcctgcttgg cgcccagcgc ggcgggcaat ccccacccca ggccgccggc gggcggcagg
    18481 taaaagtagg tacccggctg ccggcgcccg agcagctccg gaacccccca gtactcgttg
    18541 aagatcacgg tttcgtcgtc gagcgcgtcg ccgagcacgg cggaaagacc tgcccgggtg
    18601 atggtgcctt cgcgctccgc ttccgctgcc tttagcttcg cgatggccgc tttgccacgc
    18661 agggcgagct ccaccagccg ggcgcggcgg tcgggatcga cctgcccggc gcgcgaggcc
    18721 aaagcatccg cgagcccagt aagcagagcc gtaggcgtcg ccgccagggt gaggtcggag
    18781 cgatgagtgc gcatggggta acgcgagtag agaggatcgg tcccggcctg gacgatgaaa
    18841 gtttccggcc gcggggccgc gcgcgccgcg atccagggca cgtcgcactc caagaagacg
    18901 atgagatccg ccgcgtcgaa gatggattgc atctgggtgc cgaggtgaag ctcgtggtcc
    18961 atgggggcgt ttaagtaacg gggcatcatc tcggcgatgc cgatggcaaa gcggtcggcc
    19021 agatcggcaa gcacggccac gctgtcaggc tcgcagccgg acgccgaggt gacgatcacc
    19081 ggacaacgtg cggccgcgat ccggccggcc agctcctcta tagccgagga ttccggctcc
    19141 acggcaaccg gtaccgccgt ccttcgagcc ccagcatccg cgtccggcat ctgctccgcg
    19201 agcacctccc gcggcaacga gaggtagacc gggccgcgcg ggtgcgaggt cgccatgctc
    19261 aatgccctgt ccaccacctg gtgaacctgc cggggatcgc gcagctcgta gtcccacttg
    19321 accagctcgc gcagcatgcc ggcctgatcg aacatctcct gggcccagtg aatgtgggta
    19381 tctcgcgcac ccagcgcacc gtcttcgagt atgggcgtcc ggccggcggt caccagcagc
    19441 ggaacgttgt cgcgcgcggc gttggtgacg gcgcacaccg cgttggcggt gccgacgctg
    19501 acgtggaaca tcaccgcctg gggccgcccg ctgacgaggt aggccccgtg ggccatgccc
    19561 acggccaggt tctcgtgggt gcagagcacg ggtgccggca gcgcagggcc ctgggcatca
    19621 ccggagcggg cgtacgcttc gaccaggggc gcgaagtcgg ttcccgcgtt gacaaagagg
    19681 tggctgatcc cacgctcagc gaggagcgaa agataccgtg atgcgaccga ctccgactgc
    19741 atgccctttc tccgtgtgac gatcactcgc cgagcccaat tcgcggcggc cgcctaggct
    19801 atcactttac cgcagctacg cgccgggcga tcatgcccgg ctcgggagga agcgtgcacg
    19861 ttcaacccat aatttcggaa atcgccaggc aggctgatgc ggccgaccgg tcccgcaccg
    19921 tggacccgga cgtcatcgcg gccatcaagc gttcggacgt catggggctg agcgcgagcg
    19981 ccggtctcgg cgggctggac gcgcccgtga cggagatcgc ccgggaactc ggcgcggtgg
    20041 cgcccgcgtg cagctctacc gcgtggtgcc tgtggaatca tctgagcacg tttcatctct
    20101 tcgccgggtt gctcggcccg gagcatgcgg attttctcaa ggacatcgtc gagcggcacg
    20161 agtgggtctg ctttcccgcc ggcgccagca cggccgtccg ggcagaggag cacgacggcg
    20221 agttcagcct gcgcggcacc gccgcgttcg gctccggcgc ccgctatgcg gagtggagcg
    20281 gcgtgagctt cgtacccgcc gagggcgggc cgcccgagtt cagcatggtc gacctgcgca
    20341 gcgagggcgt gcgcatcgac cccacctggg aggcgatgag cctgcgcgcg tccgccaccg
    20401 accacgtgca ctacgagggg gtgcgcgtgc cggccgagcg ccgggtgccc ttccacttcc
    20461 gttaccggga agttttccgc aatcccgacc acgccatgat cgacgcgcgc taccgggagg
    20521 actgggtagc gctgtctgac ctctggctcg gcgccatggc ggtcggggtg gcccaggcct
    20581 gcctcgacga gtgctgcgac ggcatccgcg agcgggttgc catactcggg accaagatgg
    20641 ccgaacgccc gaccatccac gtgaatctgg gccaggccgc tgccgccatc aacgcggccc
    20701 gcgacacggt gttcgccgcc ctgtcggaaa ccgacgagag gctcgattcc agcgtcattc
    20761 cggacgaagc ggactatctg cgccaggtga gcgcttccat gcaggccctg cagctgtgcg
    20821 acgagtccat gcggctcatg ctgcgcgtcc tgggcggcaa cggcctccgg gagggttcga
    20881 acttcgagcg gcgctaccgc gacttccagg ccatgcccct gcacatcaac gcacacaggg
    20941 accgcatcac cgagcaggtc ggcaggcacc tgctggggct cgaaaccgag aaccccttct
    21001 gaatcgaagg gtccaacctc tgcgtcatga cgagcaattt tccgacgacg gttacccggc
    21061 gactggcctc gctcctggcc ctcgccgcga tcttcgccgt tctgggcggg tgcgggggcg
    21121 ggggcggtgg ctcctcgccc gccgtcgcgg tagccccaga gcccgagccc gagccggaac
    21181 agtggcagcc gggcgtcttc gagccggcgt ccaacttcaa ggacatgtgc gcatcgccgc
    21241 gaatcggcgc gcagtttccc gacacccagg ggacgacgac ggacgagaac aactggctgc
    21301 gctcctggag caacgatctg tatctgtggt acgaggagat cgaggaccgg gaccccgcgc
    21361 ggtacacgac ccccgactac ttcgacctgc tgaagacgtt tgcctccaca ccgacgggcg
    21421 cgcccaagga ccgcttccac ttcacgtacc ccacccagga gtgggaagcc ctgagccagt
    21481 ccggccagca accgggctac ggggctgcct tcgttctcct ggccgcagcg ccgccccggg
    21541 agctggcggt ggcgtacatc gagcccgcca gccccgcgga agcggcaggc ctcgtccggg
    21601 gagcccgggt actggagatc gacggcgtct ccgtggtcaa cggatcggac gtggacacgc
    21661 tgaacgcggg gctttacccg tcggccgccg gcgaggagca cgtcttcgtc gtgcaggaac
    21721 ccggcggcgg ctcgcgcacg gtcaccctga cttccgccat cgtcaccgag gacccggtcc
    21781 agttcgtcaa ggtgatcgac tctctgaccg gtcccgtggg ctatctgcac ttcacccgcc
    21841 acatcgccac cgccgagcga gagctgatcg atgcaatcga atttctgggg aacgccggcg
    21901 tcaccgatct gatcctggac gtccgctaca acggcggcgg tttcctggac atcgccaacg
    21961 agctggcctt catgatcgcc ggcggcggcg cggccagcgg ccgagtgttt gccgagatcc
    22021 agttcaacga caagcaccag acggtcaacc ccgtcaccgg cgcggtcctc gagccggagt
    22081 actttcacac caccacccag ggcttctccg ccgaagccgg cgaccccctg cccacgttga
    22141 acctggaccg gctctacgtg ctcaccaccg ccgacacctg ctcggcctcc gagtccatcg
    22201 tcaacgggct gcgcggcatc gacctcgacg tggtgcaggt cggatcgccc acctgcggca
    22261 agccgtacgg cttctacgcc acggacaact gcgggacgac gtatttttcc attcagttca
    22321 aagccgtcaa cgcgaagggg ttcggcgact acccggacgg cttcgccccg gaagatggcg
    22381 cccctgccgg cgccgtgacg ctgcccgggt gcggcgtcga cgacgacttc gacaacccgc
    22441 tcggagatcc ggccgaggcc ctgctggcca cggcgctgga gcaccgcgca ggcctgggct
    22501 gcccggtggg cacggccaca gccgccaggg cggagccgcg tctggcccgc aaaggctcgg
    22561 gtggcgagcc gctcacgccg ccaaggccat acggcatggt gctggcacgt tgagatgaga
    22621 aaaatctgcc tgatcccgct cgtgttggcg tccgcttgtc agagcaccac tgcggagcag
    22681 ctcccggcgg ttctgatcgg cgaccgcctc gaggccatcg cggagatcac cgaggtgatc
    22741 gccgacgcgc tgcgcacgga agtttcgctg tctccgtcgg ctttctcaga atcgagcctt
    22801 ttgacgctgg agcacgccgt gggctccgat gcccggggaa gtgctgccac gggattgctc
    22861 cttgagcggc ccgagcagtt tcggctgctg tggtcggacg acggctgctc ccttacgcgc
    22921 ctgcgcaccg gaaagaccct gccgctggat gccgccggct gccgtcccga ggcggaggcg
    22981 acagcgggcc gtgcacaatc cccttagctt tcaatcagga ttcggccagc ggcggcaagc
    23041 cgtggcgtca gcgtcctgcg ccggcgtgct ttctgtacta gactgggttt gcccgccggg
    23101 acttgcaaac ctcctggctg ccagcatcga caccttatca gggacaagcg ggatgaatga
    23161 gctgccgaag gaagcgacgg atcagacgga tcggggcgtt cgcgacctga tcacggcggt
    23221 gtcacgcgta agcgcttggc acgatagcgc catcgagtac gagaaggtga ccggtggaat
    23281 caccaacgag aacttcaaag tccgcgccga tggcgccact tttttcgtca agatccccgg
    23341 tagcgggacc gaagccttca tcgaccgggc gaactgccac gtcgccaatc agatcgccgc
    23401 cgaggtcggc atcggtccgg cggtcgccca tttcttcccg gacacgggcg tcgagatctt
    23461 cgactggctg gacggttacc ggacgatgaa ctggggcgac gtgtacgaca atgatgtctt
    23521 ccttggcacg gccgatctca tcagaaaatt ccagacgttc gaagatctgc agatgcccct
    23581 ggtccagacc gcattcgaac agacgttcga gatgatccgc atggcgcgcg agatgggcac
    23641 ctacctgccg cccgagatgg atcggatgga atacctcgcc catcagatcg aggcggccgt
    23701 cgaacgcggc ggcatcgagt accgcccctg ccacaacgac tattactcga tgaacatcct
    23761 catccatgaa cgcaccggcg agctgaagct ggtggattac gagtacgcgt ccatgaacga
    23821 cgagtgctat gacatgggcg tctattccgg ggccaactac ttcaccgaag agatggacgt
    23881 gatgttcatc aaacgtcgct atggcggttg ggacgaggag cgcttcgcgc gtctgaagct
    23941 ctacaagctg ctggccgaca tcaagtggtc catgtgggca ttggtccagg accgaatcgc
    24001 caccgtcagg tttgactact tcaactggtt cggaaccaag atggcgaggc tgcgcggcaa
    24061 ctccctcgac ccgcgcatcg actactggct gaacctcgta ggtcgcacgg gccggctcaa
    24121 cgcgtaaacg cggtcacgag ctcgatcagc atctgcacgc cgcgaccgtg cagatagcca
    24181 tcgacaacca ccgcctgcag cgggttggta ctccaggctg cgggcgtcgc cggaacgacg
    24241 tccatatgat gcagcagcac gaggccgggt tggccaccgc cagcgctccc ttgaggagga
    24301 ctcgatgagc gttcatgcgg agactcggtt cctgagagga agagcgtgcc cgcttcgctg
    24361 tcgacgaaac agatggcgcc gtctgtcgct acgccgtcga tcgtcaccga ctttgcagat
    24421 gagtgctgat gcccaaggca ccgcccgatc cgcccgcagt gctagcaccc tctcttcggg
    24481 tggcacttgg tgcccccgcc atcgcctgcg cccccggagg tggtcacggt gacctgcacc
    24541 caagcggtcg gatctgacgc gccaaaggca ttcaccgcac ggacgcggta tctgaacgtg
    24601 cccgagccgc tgacatcggc gtacgaagtc gtatcagcgt ccaggctgat cgtcgtgaaa
    24661 ccgcgccagc cgttgcgttt gtgctgcatc tcgcgatcga cctcgaaggc ggtctcgttg
    24721 tcgctttcgt cggtgaactc gatgagcgcg gtgccatcgc cctggtcggt gactgcgagc
    24781 ccgatcggcg ctgccggcgg atctcccgat ggtggtggtc cgcccgcgag gtccggcctg
    24841 agcacgaaga gtccctgctc gatgccgctg accaccacgt tgccgctggg aaagtaggga
    24901 tagacgctcc aagcgccgtt gtacgaagcc gagttgctgc tggggtagac gtcgaagtag
    24961 gcgacctcgg agaacgccgc agcggaagga tcggtgagct ccaggatgcg cagacccgcc
    25021 cggtagttgg cctggtagac gtggttcccc aggacgtact ggttgtggtc gatggcgggc
    25081 gtggtgccgt catgactgcc cgcataggcc ggctggtcga ggttctccag cttgaaaacg
    25141 cgcgtccggg tgttgtgtcg aaagcgctgc tcgtcgagct cgtcgccgaa gacgaagtag
    25201 ctatggtcag gcgtgagcca gccctgatgg gtataaccgg accctggata agtcacctgg
    25261 ctgaggacag cgggtgccgc cttgtcggtc acatcgacga tgacgagggt gtcttcgttg
    25321 ctgttgatgc agatctcccg atcgaagtaa ttcagatccg gaccctggta gacgacgcag
    25381 tgcgcgtcgt gggtataccc cgcactcgaa tagcatccag cgccccgagg gttcgtggga
    25441 ttcgagatgt ccaccatgtg cagaccgcca ctgcaggtgt cggtgccgac ggcaaacgcg
    25501 tagccgctgt cctcgttgat gacgatgttg tgggcgctgc cgaaggcgtc gtaatgggcc
    25561 gcctcggtga tgtcgggcga ggcgtcgagc agcctcgtca gatcgaacac ctgcatgcca
    25621 tgcccgtccg cctcgcttac gatgaacgcg tgatccgagt aggtcttaat atcgcgccag
    25681 gtcgaattcc agttcctggt gggaagcttt cccagaacga ccggattggc ggggtcggta
    25741 atctccacga atccggtgcc gtcgtttaga ccaatcaggg cgaactcgcg tcccgagatc
    25801 gagtcggtcc agccccagac gtcgtttgcg ctggcgctgg agagcccggc cgtactcagc
    25861 ggaacaagcg cctgtaggtg gacgttgtgg cagggaaagc cgtcggcaaa cccgtccacg
    25921 cacccaacgc tgctcgccgc cgacagcgcc tgggcgctgt gcgcatcggc ctccatgcgc
    25981 ctcatcagcg ccgtcgggtt ggccggagaa cccttgaagt acgggcgtcc gcgaccgtcg
    26041 tcgtcggcaa cggcgggctg gatcagccag caaacgatga ggcaacaggc ccaccagaac
    26101 ggcttctgcc ccggcatcca ggtgacatcg ctgatcatgc ttctcatctc caccccgacc
    26161 ttacagcgat cactcagata tataagaaac taaatttaca acatgttccg atcagccaca
    26221 atccgttcgc gagaggcaca aaaaagcccg cactgggcgg gctttgcgga cgatcctgga
    26281 cttgcccgga tcgcgtattg gtggaggcgg cgggagttga acccgcgtcc gttggcactc
    26341 ggctgttggc tctacatgct tagcttccgt ctattgattt agcctcgagc gacccgacgg
    26401 tcagggtgct gtcagcgagc ccgattggtt ttaacgcttc agccccgggc gagacgtcca
    26461 cgcgatcctg caaactttgc cgtcgacttc gccttgcagg ccaagctcgt cgacgtaagc
    26521 gggtttttta ggccgctagt gcgtagtttt cgtcgttggc aactatatta agttacagcg
    26581 attgtttaac gagagtcact accctctcgg catgcaccgc aagctccgtt acccccgtcg
    26641 aagccaaatc gcccccattt acgaggtgac gcgaattgta tcacgcgtca aatataacgt
    26701 tactgcataa taactgactg atttcacgag aattggttcc cctatcaaca tgccccgttt
    26761 tggtgttttg aaacctgtgc tggccgccgc actcgccgcc gcactcgccg ccgcactcgc
    26821 cgccacaccg gccgcagcgc aggcgccagc ccaggagccg ccgctggatt cccgcagctt
    26881 catagagcgg tcggtaacgc tgctgaaaga agaagagtac gcgctggcca gaacctatct
    26941 cgcgccggca ctcatcgact atcgcctgac accgggcgag cgctcccgcg cctactacct
    27001 gcgcggcttc accttcttcg ccgagggctt ctacgtctcc gcaggcaaag actacgcgcg
    27061 ggccctggag ttcaacccgg acaaccccgt ggccctggcc gccatggccg acctctactt
    27121 ccggggcctg ggcgtggagg cggattacgt catcgcctac cagctctact ccaaagcggc
    27181 cgccgacggc cacgcgaacg cccagttcca caccggttac ctggaactcg aaggtatcgg
    27241 catcgagaag aacctggaca gtgccagggg atggctgcac aaagccgcag atcaggacgt
    27301 ggtgcccgcc atgattcaca tggcacgcag ctaccggaca gaggccaccg acagcccgga
    27361 tccggaccag gcccgagcct ggtacgagag ggctttcgcc gcgggaagcg cagacgcgct
    27421 ggtggctctg gccttcatgc accaggacgg ggacttcggc gcgccggacc ctgaaaaggc
    27481 gctggagctc ttcacccggg ctgcccagga aggctccggg gccgcccagg tcagcctggc
    27541 gcacatgcac atcaccggcg agctgctgcc acaggactac gccaaggccc tggagctgct
    27601 tcagcaggcg gcaaggcagg gggtgccggc cagctacctg gggctcgggc acatttacga
    27661 cacgggcctg ggcgtgaagc ccgatcgcgc gacggccgag aactggtacc gactggcggc
    27721 tgaggccgag ctgctcccgg cgcagctccg cctggtcagg ctcctgctcc gggaggacga
    27781 cagcacctcc gaccgggaag ccatggcgtg gctcgccagg gccgcccgac aggacgaccc
    27841 acaggcccac aacgactatg cgtggctgct tgctacccac gaggaagccg accttcgcga
    27901 cggcgacctg gccatcgagt ttgccgagcg cgctgtcgcc cgcgagcgca taccaagcta
    27961 tctggacacc ctggcagccg cgtacgccga agccggccga ttcgaggacg ccgtcagcac
    28021 ccaggaacag gccatcgcga tgctgaacga agcggagggg gagattcgcg cggagcttgc
    28081 cgagcacctg gatgcatacc aggccggcag accctggcgg gaataagggg tttctggtac
    28141 aaaacgcgcg gctgaaaagt cagcattcga ggaccaacgg ttgagcatcg aacaacgccg
    28201 caccaagaga atcttcaggg tgatccagga gatcgtcggg gcgaagggcc gcgatttccg
    28261 tcctggcgat atcgcctcgg tgctgcgcga gcgcggccag ccgctcgggg cgtgggaaat
    28321 tcgcggccag ctgtccattt tggaggcaga ggggctcatc cggctcgtac cggaaagtgg
    28381 cagctgggag atggcgtcct ccgagaacca ccgcgcggtc agctgatcgg attcgccacc
    28441 ggcgcccgag catcgtttcc ttgcgccccc aacccgtgcc ggttaccgtt tggtgatggc
    28501 gaaaacctcc gagacccccg acgacctttc cgacgagctc gatttcgaat cggcgctcga
    28561 ggacctcgaa tccctcgtgg aaagcatgga gtccggcgag atgtccctgg aggaatcgct
    28621 gcgcgccttc gaacgcggca tcaagctgac ccggcactgc cagtccgcgc tgaaagcggc
    28681 ggagctcaag gtacgcatgc tcaccgagaa cgacgagctc gttgcgctgg acgccgacga
    28741 cccgccaccg gaatgactcc cgaactcgac gagctacgcg cgctcatcaa cgacgcgctc
    28801 gatcaccacc ttcccagttc ttccgccatc gcccaacccc tggtggacgc catgcgttac
    28861 accaccctgg gcggaggcaa acgcatccgc ccgatgctgg tgtgcgcaac ctgcaccggg
    28921 ctcggcgggg cgctggaaga cgcgctggcg ccggcgtgcg cggtggagtt catccacgcc
    28981 tactccctga tccacgacga cctgccgtca atggacgacg acgagctgcg ccacgggctg
    29041 cccagcagcc acatcaagtt cggcgaggcc acggcggtgc tcgccggcga cgccctgcag
    29101 gcgctggcgt tcgagactct gtcgagggca ccctcggggt ccgacgccca gcggctgcgc
    29161 gccgtgcagc tgctcgccag agccgccgga ccccagggga tggtgggcgg gcagagctac
    29221 gatctggaat ccgagcggaa gaagctgagc ctgagccagc tgcagaccct gcacgcggcc
    29281 aagaccggag cgctgatcac ggcggcggtg gagatcggcg cgcttttcgg caagcccgat
    29341 ctttcccccg agaaacacgt gctcatctcc gagttcgccg ccaggatcgg tctcgctttc
    29401 caggtcatcg acgacatcct ggacgtcacg gccaccacgg aaaagctcgg caaaccagcc
    29461 ggctccgaca cgggagcagg caagagcacg tttccttcgc tgatcggcgt cgagtccgcg
    29521 cgcgggatgg ccgaagagct gctggccgaa gcgctgccca tgctggatcg ggtcggtatc
    29581 aagcactcgc tgctcaagga tctcgcggag ctgatcgtca agcgggcctt ctgaaccctc
    29641 gctcagtgct gcttggtgag ggtgagcacc cccgcgtttt cctgcaggct cacctggcgc
    29701 aggaacgaca tgcccagcag cggctcgagc gggtactcgc cttcgatgat ggcggcgcgc
    29761 acgttgtggg ccgtgatgcc accgaccaca atctcatcca acgtgacgaa atgcgtgtcg
    29821 gcagtgccct gcgcggtctg caccgtgccc tggtgcgact cgtcgaactc gatgccca
//
LOCUS       DBNHLM010000012        16675 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_181001, whole genome shotgun sequence.
ACCESSION   DBNHLM010000012 DBNHLM010000000
VERSION     DBNHLM010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 16675)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16675)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16675
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_181001"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1256
                     /locus_tag="AC16QF_00615"
     CDS             93..1256
                     /locus_tag="AC16QF_00615"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6447080.1"
                     /translation="MHEREFVERIRGLVPMLEAHAGEAERDRKPVDSVMAAVEETGAY
                     RFFVPKRYGGWEFGLDTFMDVGLLLGEGCVSTAWVVTFCMEHNWLLGLFNQQAQDEIF
                     GEFPYIIAPGTLAPRGTAEPVDGGYRVSGRWQWGTGVMHANWVMVGALTPSADPEKPD
                     LCMYLIPREEVDVLDTWHVAGMVGTGSNDIEVKDVFVPGHRMQSVSHMRDGGSPGAAL
                     HGTPTYRMPMMPVLGLTAAAPAVGGARRAVALFRELLGERNVYGTTEKQGQRAIAQAR
                     LAHAQIAVENAQILLKDTARRVMEWGESGERCPDVERAHLRLKIATVVRQARDVVQQV
                     VEACGAGAHFLDSPLQRIQRDLNTLSCHTVFDMDVGGELYGRLLLGLPANAPV"
     gene            complement(1271..1723)
                     /locus_tag="AC16QF_00620"
     CDS             complement(1271..1723)
                     /locus_tag="AC16QF_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024854.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6447081.1"
                     /translation="MAPSTSDPDRSYGYQSRATHRAFDRLLQQHLRQHGLTNGYWYVL
                     RVLWEEEGMSQRELARASNLTESSAVIMLDGMQRAGLVARRKDPADGRRQRVFLTAKA
                     KRLKPKLLPVAGELNALAASGIAAEDLATYLAVAHRMRANLVAALETG"
     gene            complement(1725..2345)
                     /locus_tag="AC16QF_00625"
     CDS             complement(1725..2345)
                     /locus_tag="AC16QF_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR6447082.1"
                     /translation="MFYDPREDPQPEPLGHNPINALVAPRPIGWIASLSADGAPNLAP
                     FSYFNAFSADPPVVAFAPNEKSGAGGPKDTLANVRSVPEFTASLVSEPLARRMNESSR
                     VLPHGVSEFIEAGLTPAASRLVAPPYVGEALAALECRVFDIIELPGRPNGRRSHLVIG
                     EVVGIHIDDGVIVDGRIDSLALAQLARLGYHDYTVVRELIEIPRPD"
     gene            complement(2366..3475)
                     /locus_tag="AC16QF_00630"
     CDS             complement(2366..3475)
                     /locus_tag="AC16QF_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6447083.1"
                     /translation="MSDRAPLIVDRTGEPLTTSEPARSKPARDWPPITITREMIDEEI
                     ERLADLPQPANGRRQAMFVHPNAPDPGRGLTPGVQVTLDVLKPGEETVPVRHNSTQVS
                     FCIRGGGTAIVGDRPIRYAQYDAWNHPSYATYRHVNDSRELQARLTYSNAALLEMMRV
                     HLVEEDPQDEPVARSAQAPAATGAQTETFRIGEDGAALMPYETLINPPSVASDALHWP
                     WRTVKANLDRLEALGADYVGRRLYLLFNPRTERFNGTTPNFFATITIRPPGIVDKPHR
                     HVSSAINYYFNGSGYSRVEGKRYDWKAGDLMVSAPGWAVHNHASNEGDPVYELTIQDQ
                     PLNIYMESLLWQEDLAEPARILGSHEGFSTNRSTL"
     gene            3575..4807
                     /locus_tag="AC16QF_00635"
     CDS             3575..4807
                     /locus_tag="AC16QF_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6447084.1"
                     /translation="MDVDGATWSWPDGGVTEVPYAIYTDPGIFAREMERIFSGPSWAY
                     VGLEAEIPQPGDYLVTRIGVRSVVIARDEEGAIHGFANRCAHRGVKVCRQRHGNARFF
                     VCPYHQWSYDARGRLRGVPFINGVRQQGGMPEGFAREDHNLPPLDIATRGGVIFASFS
                     ATAPRLEDYLGDTNLSYFDRVFDGRQLKVLGYSRQRIPGNWKLMFENIKDPYHASLLH
                     VFLVTFGLFRADQPAQVRMDPSGAHGLLISRRGEQGATADTGEMSSMKEDFSLADARL
                     LDPVKEFPDDATVVMQTIWPNLIVQQQSNTLALRQIQPLDPGRFELHWTFFGYGDDDD
                     AMTLRRLRQANLMSSAGLVSADDSEVIKLAQDGVAQYPDDVGVMLMGGRGTDDEDHMV
                     TETAIRAFYAHYRRVMGL"
     gene            4804..5283
                     /locus_tag="AC16QF_00640"
     CDS             4804..5283
                     /locus_tag="AC16QF_00640"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013062.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HIR6447085.1"
                     /translation="MTLAAEERAELEEVYARYADLLDDGPLADWPALFHEECLYLLIP
                     RENHDRGLPLAIMRCESRGMLEDRVRAVQETIMHEPRYLRHHITNVRGARTDDGGYSV
                     IANYSVIEVLQDELPRILSVGRYLDVLTRSPTGALMFREKRVVFDSVMVPNTIVYPL"
     gene            5293..6672
                     /locus_tag="AC16QF_00645"
     CDS             5293..6672
                     /locus_tag="AC16QF_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HIR6447086.1"
                     /translation="MTLSDRLLDLGFLEAETPRTPMHFSALMFFDTPSDPAPFVSGLR
                     EAWTGAVPVAPFDLVPVHDLKSGLFLPRLAPAERVDVEDHLHLVTLPAGSGEQALLEL
                     AQRLHAECLDRSRPLWDVYLITGFGERCFAMFVKIHHAIFDGHGGLRLFVETLEEAPV
                     TRLPEPFWSRPSAAAPGHGRPLGPFEAVGALARSAGGLFRNAAVGARHASRWLLGSAP
                     VAPPAFSEPASILNGPSSGRRRLAHLSLPLPEIERCAADHGASVNDVLLAICGGALRR
                     YLEARSALPDRSLTALVPCSLRADAGRLPAGNRLTFIPMPLGTDIAEHPDRVSAVRAG
                     AGEAKERLEELGDPGALQYGMLINAVATLPRLARLPLPPISPANLVVTNLAGPAAPRH
                     LGSARMTQIYAHAPMSDGVGLNIALVSYAGEAFLGLTADPSLVDDVDELARLLARTAS
                     ELLGGSEAA"
     gene            complement(6673..7602)
                     /gene="nudC"
                     /locus_tag="AC16QF_00650"
     CDS             complement(6673..7602)
                     /gene="nudC"
                     /locus_tag="AC16QF_00650"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HIR6447087.1"
                     /translation="MSHVHIFAGNPLDRADALRRDPEWLDGARADPGSRYLPFARLNV
                     LLCGERPELGWLRSSEVQRLGISVPPIFLGLREGIAHFAIDVSELGDPAHELNLDDSW
                     RFEDCRAAGTVLSVPDSGIVAQSRSQIDRHRRHQFCSVCGSRSEPERGGHVRRCPSCS
                     AEHFPRTDPVAIMLITNGDECLLGQAAGRLSRLGMYSALAGFIDQGESIEEAVRREVR
                     EEAGVRVGAVRYHSSQPWPFPSSLMIGCHGTALSTDITIDPSEMADVRWFSRDSVRLA
                     LQDDNPELRVPGPIAIAHHLIRAWADGEVDLDT"
     gene            7802..8095
                     /locus_tag="AC16QF_00655"
     CDS             7802..8095
                     /locus_tag="AC16QF_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447088.1"
                     /translation="MADRMISWTFYGLACLCGIAAVLILLVGFVDWLQTERWTNLSVL
                     EFGYDAHLIRARWFINNRWSWWLHDLMHLIPLYVFLLALAPLSWVVGAWFGRR"
     gene            complement(8120..8476)
                     /locus_tag="AC16QF_00660"
     CDS             complement(8120..8476)
                     /locus_tag="AC16QF_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447089.1"
                     /translation="MSERDEQEKLEEQACALLLRSPHLSVSQVLDLMDIGDSEFREML
                     RSNPRIASLMEQRREGALPRVESKMTECPACGEWFIPYASARCCSDTCAKIDQISNRP
                     NAARRFRLRNRQTGGS"
     gene            complement(8612..10240)
                     /locus_tag="AC16QF_00665"
     CDS             complement(8612..10240)
                     /locus_tag="AC16QF_00665"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6447090.1"
                     /translation="MTYPWNYGDLLDAVEHITPGSRAALIHGDRIVSWADFSRRTNNL
                     ARALLNGGAAPGDKIAFYMRNRPEYSEGIAAGFKARLTHVNVNYRYVDHELIYLLDNS
                     DAAVVVYAAEFAHHVAAIRDQLPGVGQWIEVPDGHPGLDDALDYERLVADGDGAPLDL
                     DRSPDDMLFLYTGGTTGMPKGVMWRHQDLWCVTGAGGNPRIGVEPSPDLQSYVSRLQA
                     EPPPVNLPLPPIMHGTGLLSVVGAMAHGGTAVTLTGRSFEAEAALEAIQRHAVTSTTI
                     VGDAFARPMVEALDAAPGRHDISSLKIVSSSGVMWTRAIKAGLLRHNPDLVLVDAFSS
                     SEAIGLGTSVMTRDEEIEVAKFTLGPSCKVFGEDLREIAPGSGETGMVGVTGFLPVGY
                     YKDEEKTARTFPVVDGVRYSIPGDWVRVEADGSLTLLGRGSNCINTAGEKVYPEEVEE
                     ALKHHESVADALVVGLPDERWGQAITAVITLHPGYSLDEIGLREFARRELAGYKLPKR
                     ILAKDDLERAPNGKANYAAIREFAQAELGISSGS"
     gene            10440..11978
                     /locus_tag="AC16QF_00670"
     CDS             10440..11978
                     /locus_tag="AC16QF_00670"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6447091.1"
                     /translation="MEVRTLMARAAAHYADREAVIHGDRRLTFREAWQRSLRLGNGLR
                     GLGLTPGDRIGVLEDNSVEAADLFGGAAVANLVRVPLYPRNGRDAHRHMLGHTNCRAL
                     IVSENHAEEVAGLADELPDLEHVIIRDAGYDGWLAAQTDEDPRLPISSDDYFIIRHTG
                     GTTGLSKGVAYTHKAWLAAGRDWFYAFPPVEPGDKCLHLGPISHGSGYQYLPIWLAGG
                     CNVMMDHFDTGETLDLIEREGIAYGFMVPTMVNAVVHDNSARGRDFSSLKCLLIAAAP
                     IQDATALAAREVFGDVLWQGYGQTEVVPVSMMGPRQWFAEVEGSSPLRACGLPLPFAE
                     VQIWDEDNQPVAAGEVGEIVARADGQMTGFWNNPEASAERIVDGWVKTGDLGRLDANG
                     YLYVVDRADDMIISGGYNIWPAELENVLASHPAVIEVAAFGVPSQKWGESPYAVCVVG
                     NAEAVGEAELIDLVAEELGSYKKPVGVTITTDPLPKSPVGKIKRKDLREPFWKGHERR
                     VAGS"
     gene            complement(11997..12413)
                     /gene="arfB"
                     /locus_tag="AC16QF_00675"
     CDS             complement(11997..12413)
                     /gene="arfB"
                     /locus_tag="AC16QF_00675"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HIR6447092.1"
                     /translation="MSVSRIRQIPPEACSERFVRSPGAGGQNVNKVATTVQLRVDLDR
                     ANLPPAVRERLEKLAGRQVNQRGELVITASRFRTQARNRADAMSRLSELLEQAQRVPR
                     RRVATRTPAGERVRRKDTKRKRGTTKRLRARPPMDD"
     gene            complement(12462..13592)
                     /gene="epmB"
                     /locus_tag="AC16QF_00680"
     CDS             complement(12462..13592)
                     /gene="epmB"
                     /locus_tag="AC16QF_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HIR6447093.1"
                     /translation="MSRRPTRDRDRLRYETADDCAILVPDVNPMTQSAPEQSAARLRT
                     RTVADDPATGSWQEALRNAIRTPRELIEALELDPAWAPVMDGHDDDFPMLVPRSFAAR
                     MRKGDPHDPLLRQVLPDGAERITAPGFEADPLQEQRFARAGVVRKYSGRALLIASGAC
                     PVHCRYCFRRNFPYADQLASRQRWTGAVDALSADTQVTEVILSGGDPLTLTNEAIGEL
                     IRAMENVASVQTLRFHTRFPIIIPERVDAGLVDTLAATRLRTVFVVHCNHANEIDASV
                     RRALADLKAAGAQLLNQSVLLKGVNDNEDDLAELSQRLFDAGVLPYYLHLLDPVAGTA
                     HFDVAPARGRALVAGLRRRLPGYLVPRLAREVPGESSKTILA"
     gene            complement(13589..14893)
                     /locus_tag="AC16QF_00685"
     CDS             complement(13589..14893)
                     /locus_tag="AC16QF_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR6447094.1"
                     /translation="MKFSARTATAVIMANMIGTGVFTSLGFQLADIRSEFVLLMLWAV
                     GGLTALCGALSYAELGAALPRSGGEYNFLGRIYHPAVGFVSGWISATIGFAAPTALAA
                     ITFGIYLASVFPALSPVWLACGLVVVLSVAHATSRRSSGGVQRAFTAVKIILILGFCL
                     LAALLVDEPQGISLLPHEGDGALLTSGAFAVALIYVNYAYTGWNAATYLTGELDRPEQ
                     TLSRVLLQGTVVVMLLYVLLNATFLYVAPAEAMEGRLEIGYIAAEHAFGSNGAAIMAV
                     ILALLLISTASAMIMAGPRVLQVIGQDYPAFRFLARVSGDGVPVTAIVVQSVLALAFI
                     LTASFEAILVFAGFTLGVSTFFTVLGLFVLRIRQPDLPRPYRTTWYPLPPLVYLAITG
                     WTLGYIIAHRPKEGLWGLAIIAGGALLYLLIRRGRPTEEVSS"
     gene            complement(14937..15377)
                     /locus_tag="AC16QF_00690"
     CDS             complement(14937..15377)
                     /locus_tag="AC16QF_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447095.1"
                     /translation="MARLALTGAIIILLSGCAEPFIVFAGGALSGDEKVPPADWSFLE
                     AIDTVQIETDPNDPYSVNVWSVGIGADVYVATGEDGTNWTERIDGDPSVRLRTNGDIY
                     PLRATAVTEMAERRRVAQAYLNKYDVDDDEGWVDTGKIYRLDRR"
     gene            15507..16487
                     /locus_tag="AC16QF_00695"
     CDS             15507..16487
                     /locus_tag="AC16QF_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR6447096.1"
                     /translation="MRAMQLTEPGGDFRLVDLPKPRPAAGEVLLAVHACGVCRTDLHV
                     VDGDIVDGRYPVVPGHQIVGVVEQAPDGDLQPGQRVGVPWLGHTCGTCVFCLAGQENL
                     CDSAEFTGYHRQGGFAEYAVADARFCFPIPESYPDVQAAPLLCAGLIGYRALGMVGDA
                     RKLGFYGFGNAAHILTQIAVWEGREVSAFTRPGDAAGQRFARGLGAVWAGDASTPPPE
                     PLDAAIIFAPDGALVPAALKHVRKGGVVVCAGIHMSDIPSFPYADLWGERIIRSVANL
                     TREDGVRLLEVAPRVPVRTEVQVFPLERTADALDALREGRVEGGAVVQVR"
     gene            complement(16484..>16675)
                     /locus_tag="AC16QF_00700"
     CDS             complement(16484..>16675)
                     /locus_tag="AC16QF_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447097.1"
                     /translation="AEAAIPESYLANNSLIGPEGFVRDRLQALSESGVTALNVGFMGR
                     TSQERIRSCEQLRKLVDTL"
BASE COUNT         2668 a         5381 c         5715 g         2911 t
ORIGIN      
        1 ggcaaagact ggggcgacgc gattgccggc ggtgaagcgc tcgcggcaga gctcgacggg
       61 cacgacgtgt tggctaactg aggtaggcgg gtatgcacga acgggaattt gtcgagcgca
      121 tccgcgggct ggtgccgatg ctggaggccc atgccggcga ggccgagcgc gatcgcaagc
      181 ccgtggattc cgtgatggcg gcggtcgagg aaaccggcgc gtaccggttc ttcgtgccga
      241 aacgctacgg cggctgggag ttcgggttgg acacgttcat ggacgtcggc ctcctgcttg
      301 gcgaaggctg cgtatccacc gcctgggtgg tgaccttctg catggagcac aactggctgc
      361 tgggcctgtt caaccagcag gcccaggacg agatcttcgg tgagtttccc tacatcatcg
      421 ctcccggcac cctcgcgccg cggggaactg cagagccggt ggacggcggc tatcgtgtga
      481 gcggtcgatg gcagtggggg accggggtga tgcacgccaa ctgggtcatg gtgggagcgc
      541 tcacgccgtc ggcggatccg gaaaagcccg acctgtgcat gtacctgatt cccagggagg
      601 aagtggacgt cctcgacacc tggcacgtgg cgggaatggt aggcaccggc agcaacgaca
      661 tcgaggtgaa ggacgtcttc gtccccggcc accggatgca gagcgtgtcc cacatgcgcg
      721 acggtggcag cccgggggcg gcgctgcacg gcaccccgac gtaccgcatg cccatgatgc
      781 ccgtcctggg cctgacggcc gccgcgcccg ccgtgggcgg cgcgcgcagg gcggtggcgc
      841 tctttcgcga gctgctcggc gagcgcaacg tctacggcac gaccgaaaag cagggccagc
      901 gtgccattgc tcaagcccgg ctcgcccatg ctcagatcgc cgtcgagaac gcccagatcc
      961 tgctcaagga caccgcgcgc cgggtgatgg agtggggaga gagcggggag cgttgccccg
     1021 acgtcgagcg tgcccatctg cgcctgaaga tcgccaccgt cgtccgccag gcccgcgacg
     1081 tggttcagca ggtggtggaa gcctgcggcg ccggcgctca ctttctggac agcccgctgc
     1141 agcgaattca gcgtgacctg aacacgctgt cctgccacac ggtgttcgac atggacgtgg
     1201 gcggtgagct ctatggacgc ctgctgctcg gactgcccgc caacgctccg gtctagggcc
     1261 cgtcgctcga tcagcccgtt tcgagggcgg cgaccagatt cgcccgcatc cggtgggcca
     1321 cggccaggta tgtcgccagg tcctcggcgg cgatgccgct cgccgcaagg gcgttgagct
     1381 cgcccgctac cggcagcagc ttcggtttca gtcgtttcgc ctttgccgtc aggaagacgc
     1441 gctggcgccg accatccgca ggatcctttc tgcgcgccac caggcccgcg cgctgcatgc
     1501 cgtccagcat gatcaccgcg gaggactcgg tcaggttgga cgctcgagcc agctcccgct
     1561 gtgacatgcc ctcctcctcc cacaacacgc gcagcacgta ccagtagccg ttggtgagac
     1621 cgtgctggcg caggtgctgc tgcagcagcc ggtcgaaggc gcggtgggtg gcccgcgact
     1681 ggtaaccgta gctgcgatcc gggtcgctgg tagacggcgc catctcagtc gggcctcgga
     1741 atttcgatca gctcccgcac cacggtgtag tcgtggtaac ccaggcgggc gagctgagcg
     1801 agggcgagag agtcgatgcg cccgtcgacg atcaccccgt cgtcgatgtg gatcccaacc
     1861 acctcgccga tgaccaggtg gctgcggcgt ccgttcgggc ggcccggcag ctcgatgatg
     1921 tcgaacacgc ggcattccag cgcggccagc gcttcgccca cgtacggcgg ggccaccagg
     1981 cgcgacgccg ccggggtcaa tccggcttcg atgaactcgc tgacgccgtg gggcagcacg
     2041 cgggacgatt cgttcatccg ccgggccaga ggttcggaga cgaggctcgc ggtgaactcg
     2101 ggcacgctcc tgacgttggc cagcgtatcc ttgggacccc ccgcgccgga cttctcgttg
     2161 ggggcgaagg ccacgacggg cgggtctgcc gagaacgcgt tgaagtagga gaacggtgcc
     2221 agattcggcg cgccgtcggc ggagagggag gcgatccacc cgatgggcct tggcgcgacg
     2281 agggcgttga tcggattgtg gcccagcggc tcgggctggg gatcttctcg cggatcgtaa
     2341 aacatgtttc gggcgtcagc agcgatcaga gcgtcgaacg gttggtggag aatccctcgt
     2401 gagatccgag tattcgggca ggctcggcca ggtcctcctg ccacagcagc gactccatgt
     2461 agatgttcag cggctgatcc tgaatggtca gctcgtacac cgggtcgcct tcgttggagg
     2521 cgtggttgtg cacggcccag cccggcgcgg acaccatgag atcacctgcc ttccagtcgt
     2581 agcgtttgcc ctcgacgcgg ctgtaacccg aaccgttgaa gtaatagttg atggcggagg
     2641 acacgtggcg atggggtttg tccacgattc ccggcggtcg aatcgtgatg gtggcaaaga
     2701 agttcggcgt ggtgccgttg aacctctcgg ttcggggatt gaacagcagg tagaggcggc
     2761 gtcccacgta gtcggctccc agcgcttcca gccggtccag atttgctttc accgtccgcc
     2821 agggccagtg cagcgcgtcg gaggctaccg acggcgggtt gatgagggtc tcgtagggca
     2881 tcagcgccgc gccgtcctca ccgatgcgga aggtctccgt ttgcgccccg gtggccgcgg
     2941 gcgcctgggc gctccttgcc accggctcgt cctgcgggtc ctcctccacc agatgcacgc
     3001 gcatcatctc cagcagagcg gcgttagagt aggtcagcct tgcctggagc tcgcggctgt
     3061 cgttgacgtg ccggtaggtg gcgtaggagg ggtgattcca ggcgtcgtat tgcgcgtacc
     3121 ggatgggccg gtcgccgacg atggccgtgc cgccgccccg gatgcagaag ctcacctggg
     3181 tggaattgtg gcggacggga accgtttcct cgccgggttt cagaacgtcc agggtcacct
     3241 gaacgcccgg ggtgaggccg cggcctggat ccggcgcgtt gggatggacg aacatcgcct
     3301 ggcgtcggcc gttggccggt tgcggcagat ctgccagccg ctcgatctcc tcgtcgatca
     3361 tctcccgggt gatggtgatc ggcggccagt ccctggccgg ctttgatcgg gccggctccg
     3421 aggtggtcag cggctctccg gtgcgatcga ctatcagtgg tgcgcggtcg ctcattcttg
     3481 gcctctcgag cgggaagctg gaccggcgaa gccggcgcgt tgaaatactt tgatatcaaa
     3541 gtaatatgtg ggacgtcaac tgcggggtta gctcatggat gtcgatggcg cgacgtggtc
     3601 gtggcccgat ggtggggtga ccgaggtgcc ctacgccatc tacacggacc ctgggatttt
     3661 tgcccgggag atggaacgca tattctcggg tccgagctgg gcctacgtcg ggctggaggc
     3721 ggagatcccc cagcccgggg actatcttgt tacacgaatc ggcgttcggt ccgtggtgat
     3781 cgccagggac gaggaaggtg cgatccacgg cttcgccaat cgctgcgcgc accggggagt
     3841 gaaggtttgc cgccagcgtc acggcaacgc gcgattcttc gtctgcccct accatcagtg
     3901 gtcctacgat gcccggggcc gcctgcgcgg cgttcccttc atcaacggcg tccggcagca
     3961 gggaggaatg cccgaggggt tcgcccgtga ggatcacaac ctgccgcccc tcgacatcgc
     4021 cacccgtggc ggcgtcatct tcgcgtcctt ctccgcgacg gcgccgcggc tcgaggatta
     4081 cctcggtgac accaacctct cctacttcga ccgtgtgttc gacggccggc agctgaaagt
     4141 cctcggctac tcgcggcagc gcatccccgg gaactggaag ctcatgttcg agaacatcaa
     4201 ggatccctac catgcgtcgc tgctgcatgt ctttctcgtg accttcgggc tgttccgggc
     4261 ggatcagcct gcccaggtgc gcatggaccc tagcggcgcc catgggctgc tgatctcgcg
     4321 gcggggagag cagggagcaa cggcggacac cggggagatg tccagcatga aggaggactt
     4381 ctccctggca gacgcgcgtc tcctggatcc ggtcaaggag tttccggacg acgccacggt
     4441 ggtgatgcag accatctggc cgaatctgat cgtgcagcag caatccaata ctctggcgct
     4501 gcgccagata cagcctctgg accccggccg cttcgagctg cactggacgt tcttcggcta
     4561 cggggacgac gacgatgcga tgacgctgcg gcgtctgagg caggcgaacc tgatgagctc
     4621 ggccggtctg gtctccgcgg acgacagcga ggtgatcaag ctggctcagg acggcgtcgc
     4681 ccagtatccc gacgacgtcg gggtgatgct catgggcggc cgaggcactg acgacgagga
     4741 ccacatggtc acggaaaccg ccatcagagc gttctacgct cattaccgac gggtgatggg
     4801 gctgtgaccc tcgccgccga ggagcgcgcc gagctggagg aggtctacgc tcgctacgcc
     4861 gacctcctgg atgacggccc gctggcggac tggcccgcac tcttccacga ggagtgcctc
     4921 tacctgctta tacccagaga gaatcacgat cgaggcctgc cgctcgccat catgcgctgc
     4981 gagagccgtg ggatgctgga ggatcgggtc cgtgcggttc aggaaaccat catgcacgag
     5041 ccgcgctacc ttcggcatca catcaccaac gtccgcggtg ctcgcaccga cgacggtggt
     5101 tactcggtga ttgccaacta ctccgtcatc gaggtgctgc aggatgagtt gccgcgcatt
     5161 ctgagcgtgg gtcggtatct cgacgttttg acaagatccc caacgggcgc gctgatgttc
     5221 cgggagaagc gggtggtatt cgattcggtg atggtgccca acaccatcgt ctacccgctg
     5281 taggtcggcg cggtgaccct ctccgaccgg ctcctcgatc tcggcttcct ggaggcggag
     5341 acgcctcgca cgcccatgca cttcagcgcg ctgatgttct tcgatacgcc atccgatccg
     5401 gcgcccttcg tttccgggtt gagagaggct tggacgggcg cggttccggt ggcgcccttc
     5461 gacctggttc ccgtgcacga tctgaaaagc gggctctttc tgccgcggct ggccccggcc
     5521 gagcgcgtgg acgtggagga tcatctgcat ctggtaaccc ttccggccgg gagtggcgag
     5581 caggcgctct tggaacttgc ccagcgcctc catgccgagt gtctcgaccg gagccgcccg
     5641 ctctgggacg tgtacctgat caccggcttc ggcgagcgct gtttcgcgat gttcgtgaag
     5701 attcatcacg cgatcttcga cgggcacggc ggcctgcgcc tgttcgtgga gaccctggaa
     5761 gaagcccccg tgacgcggct gccggagccg ttctggtcca ggcccagcgc cgcggccccc
     5821 ggccatggcc gccctctggg tccgttcgag gcggtgggtg ccctggcgcg ttcggccggc
     5881 gggctcttcc ggaacgcggc cgttggcgcg cgccatgcct cccgctggct gctcggttcg
     5941 gcgccggtgg ccccgcccgc attcagcgag ccggcgagca tcctcaacgg gccctccagc
     6001 ggtcgacgcc gactggccca cctgagcctg ccgctgcctg agatcgagcg atgtgcggcc
     6061 gaccacgggg catccgtcaa cgacgttctg ctcgccatct gcggcggcgc gctgcgccgc
     6121 tatctcgaag cgcgcagcgc cctgccggac cgctcgctca ccgcgctggt gccctgctcg
     6181 ttgcgggccg acgccggccg gctgccggcc gggaaccggc tcacgttcat acccatgccg
     6241 cttggcaccg acatcgccga acatcctgac cgcgtttcgg cggtccgtgc cggggcgggc
     6301 gaagccaagg aaaggctcga ggaactgggc gaccctggcg ccctgcagta cggcatgctc
     6361 atcaatgccg tggcgaccct gccgagactc gctcgcctcc cgctgccgcc catctctcct
     6421 gccaacctcg tggtcacgaa cctggcgggc ccagctgcgc cccgtcatct gggaagtgcg
     6481 cggatgacgc agatctatgc ccatgccccc atgtccgatg gcgtaggtct caacatcgcc
     6541 ctggtttcct atgccggtga agccttcctc gggctcaccg cagatccctc gctggtggac
     6601 gacgtggacg agctggcccg actgctggct cggaccgcca gtgagcttct cggcggaagc
     6661 gaggcagcct gatcaggtgt cgaggtcgac ctcaccgtcg gcccaggctc tgatgaggtg
     6721 gtgggcgatg gcgatgggcc cgggcaccct gagctccggg ttgtcgtcct ggagcgcgag
     6781 ccggacggag tcgcgggaga accagcgcac gtctgccatc tcgctgggat cgatggtgat
     6841 gtccgtgctc agcgcggttc cgtgacagcc gatcatcagc gacgagggaa acggccacgg
     6901 ctgcgaggag tggtagcgca cggcgcccac ccggacgccg gcctcctctc gaacctcccg
     6961 gcgcacggct tcctctatgg attcgccctg atcgatgaat cccgccaggg ccgagtacat
     7021 tcccagccgc gacaggcgtc cggcagcctg cccgagcagg cactcgtcgc cgttggtgat
     7081 cagcatgatg gccaccggat cggtccgggg aaagtgctcg gcgctgcacg aggggcagcg
     7141 acggacgtgg ccgccccgct ccggctcgct gcgggaacca cacacgctgc agaactggtg
     7201 gcgtctgtgc cggtcgatct gggatcggct ctgagccacg atgccgctgt ccgggacgga
     7261 aagcacagtg cctgcggccc ggcagtcctc gaaacgccag gagtcgtcca ggttcagctc
     7321 gtgggccggg tctccgagct cggagacgtc gatcgcgaag tgggcaatgc cctcccgcaa
     7381 gcccaggaag atggggggca cgctgatgcc cagccgttgc acctcgctcg acctcagcca
     7441 gccgagctcc ggccgctccc cgcacagcag cacgttcagc cttgcaaagg gcagatagcg
     7501 cgagccagga tcggctctcg cgccgtcgag ccactcggga tcgcgccgca gcgcgtccgc
     7561 gcggtccagg ggattgccag caaagatatg aacgtggctc acggatcttc caggtaaggg
     7621 tcaggtggat gaaaggagaa gggtaaagac cttgcgcgca ttgtgaaacc gccgtcctcg
     7681 agccgttctg gaacgggttc ggcttcccgg gattcgtgaa agccgtcacg gcatcgcctt
     7741 cgcgcatccg gaactctacg cttgggtcaa tggttcatct tcagacggat cgactggcac
     7801 catggctgat cgcatgattt cctggacttt ctacgggctc gcgtgtctct gcgggatcgc
     7861 ggcggtgctg attcttctgg tcggtttcgt cgactggctg cagacagagc gctggaccaa
     7921 tctaagcgtc cttgagttcg gctacgacgc tcatctcatc agggcgcgct ggttcatcaa
     7981 caaccgctgg agctggtggc tccacgacct gatgcacctg atcccgctct atgtgtttct
     8041 tcttgctctg gcccccctgt cctgggtggt gggagcctgg ttcggtcggc gctgaacccc
     8101 cgactgggcg tggtcgacgt caggagccgc cggtctgccg gttgcgcaga cgaaagcgtc
     8161 gcgccgcgtt gggcctgttg ctgatctggt cgatcttggc gcaggtatcc gagcagcagc
     8221 gcgcagacgc gtagggaatg aaccactctc cgcaggcggg gcactccgtc atcttggact
     8281 ccaccctcgg cagagcgccc tcccgacgct gctccatgag cgaggcgatg cgcggattgc
     8341 tcctgagcat ttcgcgaaat tcggagtcgc cgatgtccat caggtcgaga acctggctga
     8401 cgctgagatg cggagaccgc agcaggaggg cgcacgcctg ctcctcgagt ttctcctgct
     8461 catccctttc gctcacgcgg ccaatactcc tgccggcaga tgactcaatc ggtgtcggcc
     8521 ggccgggtca cggccagtcg tgcgctcatg tcgccgcgca atgttgccct gggtaaatga
     8581 cgatcgccta cggcccgtcc tccagacggt attacgaacc cgatgaaatg cccagttccg
     8641 cctgagcaaa ttcccggatg gccgcatagt ttgccttgcc gttgggtgcg cgttcgaggt
     8701 cgtccttggc cagtatgcgc ttgggcaact tgtaaccggc gagttcccga cgggcgaact
     8761 cgcgcagacc gatctcgtcc agggagtagc ccggatggag cgtgatcacg gcggtgatcg
     8821 cctggcccca gcgctcgtcg gggagtccca ccaccagggc atccgccacg gactcgtgat
     8881 gcttcagcgc ctcctcgact tcctccggat ataccttctc cccggcggtg ttgatgcagt
     8941 tgctgccgcg tcccagcagc gtgagcgacc catccgcctc gacccggacc cagtcaccgg
     9001 gaatcgagta gcgcacgccg tcgacgaccg gaaaggtgcg cgcggtcttt tcctcgtcct
     9061 tgtagtagcc cacgggaagg aagccggtga cgcccaccat gccggtctct ccggatccag
     9121 gcgcgatctc ccgcaggtcc tctccgaaaa ccttgcagct cggccccagc gtgaacttgg
     9181 cgacctctat ctcctcgtcg cgggtcatga ccgaggtacc aaggccgatg gcctccgagg
     9241 aggaaaacgc gtcgacgaga acgaggtccg gattgtgacg cagcaggccg gccttgatgg
     9301 cccgcgtcca catgacgccc gaggagctca cgatcttgag cgacgagatg tcgtggcggc
     9361 cgggtgcggc gtccagcgct tccaccatgg gccgggcgaa ggcgtcgccg acgatggtcg
     9421 tggacgtcac cgcgtgccgc tggattgctt ccagcgcggc ttccgcttca aagctccggc
     9481 ccgtcagggt caccgccgtt ccgccgtgag ccatggcgcc caccacggat agcagccccg
     9541 taccgtgcat gatgggaggc agcggcaggt tgacgggcgg cggctcggcc tgcagacggg
     9601 acacgtaact ctgaagatcg ggggacggct ccacgccgat ccgcgggttg ccgccggcgc
     9661 cggtgacgca ccacagatcc tggtgacgcc acatgacgcc cttgggcatg cccgtggtac
     9721 cgccggtgta gagaaacagc atgtcgtccg gggagcggtc gagatccagg ggcgcgccgt
     9781 cgccgtcggc gacgaggcgc tcgtagtcga gagcgtcgtc gaggccgggg tggccgtcgg
     9841 gtacttcgat ccactgcccg acccccggca gctggtcgcg aatggcggca acgtggtggg
     9901 cgaactcggc ggcgtagacg accactgcag catcggagtt gtcgagcagg taaatcagct
     9961 cgtggtcgac gtagcgatag ttgacgttca cgtgggtgag ccgggccttg aagccggcgg
    10021 cgatgccctc gctgtactcg ggccggtttc gcatgtagaa ggcgatcttg tcgccgggcg
    10081 ccgctccgcc gttcagcagc gcccgggcca gattgttggt tcttcgggag aaatcggccc
    10141 aggagacgat gcgatcgccg tggatcaggg cggctcgtga ccctggcgtg atgtgctcga
    10201 ccgcgtcgag caggtcaccg tagttccacg gataggtcat cgcccgtgcc tcgctaacaa
    10261 tgacgtgaag ctcatctgtt gatcccaatg ctcggaagtg tccctgcgga cgatgaaata
    10321 cccgacattg ccatgccgat caagggtggt aggcttttgc gcttgtcctg ccgccggtct
    10381 gagcggcgcg ctggtcggtc gccctgctga ggcgatcggg cccggaagga ggggagatca
    10441 tggaggtccg tacgttgatg gctcgggcgg ccgcgcacta cgccgatcgc gaggcggtca
    10501 ttcacggcga ccgtcggctg accttccgcg aagcctggca gcgttccctg cggctcggca
    10561 acggccttcg tggcctgggg ctcaccccgg gggatcgcat cggcgtttta gaggacaact
    10621 cggtggaggc ggccgacctc ttcggcggcg ctgcggtggc gaacctagtg cgcgtgcccc
    10681 tttacccgcg caacggccgc gacgcccatc ggcacatgct cggccacacc aactgccgcg
    10741 cgctgatcgt cagcgagaat cacgcggagg aggtggccgg gctcgcagac gagctgcccg
    10801 acctcgagca cgtcatcatc cgcgacgcgg gctacgacgg ctggctcgcg gcccagaccg
    10861 acgaggaccc gcgtctgccc atctcctcgg acgactactt catcattcgc cataccggcg
    10921 gcacgacggg cctctccaag ggagtggcct acacccacaa ggcctggctc gccgcggggc
    10981 gcgactggtt ttacgcgttt cccccggtgg agccgggcga caagtgcctg catctgggcc
    11041 ccatctccca cggctcgggc tatcagtacc tgcccatctg gctcgccggc ggctgcaacg
    11101 tgatgatgga ccacttcgac accggggaaa ccctggatct aatagagcgc gaaggaatcg
    11161 cgtacgggtt catggtgccg accatggtga acgccgtcgt ccatgacaac tcggccaggg
    11221 ggcgcgattt cagcagcctg aaatgcctgc tcatcgcggc cgcgccgatc caggacgcga
    11281 ccgcactggc ggcccgtgag gtcttcggtg atgtgctctg gcagggctac ggtcagacgg
    11341 aggtggtgcc ggtgtccatg atgggcccgc gccagtggtt cgccgaggtg gagggctcca
    11401 gccctctgcg ggcatgcggc ctgccgctgc cgtttgcaga ggtgcagatc tgggacgagg
    11461 acaaccagcc ggtggctgcg ggcgaggtgg gcgagatcgt ggcccgcgcg gacgggcaga
    11521 tgacgggttt ctggaacaat cccgaggcga gtgccgaacg tatcgtggat ggctgggtca
    11581 agacgggcga tctcggcagg ctggatgcca atggctatct gtacgtggtg gaccgggcgg
    11641 acgacatgat catctcgggg ggctacaaca tctggccggc ggagctcgag aacgttctcg
    11701 ccagccatcc ggccgtcatc gaggtggcgg cattcggcgt gcccagccag aaatggggcg
    11761 agtcgccgta tgccgtttgc gtggtcggga atgccgaggc ggtcggcgag gccgagctca
    11821 tcgatctggt tgccgaggag ctaggcagct acaagaaacc cgtcggcgtg accatcacca
    11881 cggatcccct gccaaagagc ccggtcggga agatcaagcg caaggatctt cgcgagccct
    11941 tctggaaggg acacgagcgg cgcgtcgccg gcagctgacc ggttcaggcc tggccctcag
    12001 tcatccatgg gcggcctggc ccggaggcgc ttggtggtcc cccgcttgcg cttggtgtcc
    12061 ttgcgtcgta cccgctcccc ggctggcgtt ctggtggcaa cccggcgtcg gggcacgcgc
    12121 tgcgcctgtt ccagcagctc gctcaaccgg ctcatcgcgt cggcccggtt gcgcgcctgg
    12181 gtccggaagc ggctggccgt gatgacgagc tccccgcgct gattcacctg gcgcccggcc
    12241 agcttctcca gtcgttcgcg gacggccggc gggaggttgg cgcgatcgag atcgactcgg
    12301 agctgcaccg tggtggcgac cttgttgacg ttctgcccac ccgcgcccgg gctccggacg
    12361 aaacgctcgg agcaagcctc gggcgggatc tgcctgatac gtgaaacgct cattggccaa
    12421 tcatagcagc ggcgccttct tcccagacgc gggccggcac gctaggcgag gatggtcttg
    12481 ctcgattcgc cgggcacctc gcgtgccagc ctgggcacca gatagccggg cagcctgcga
    12541 cgcagaccgg ccaccagggc acgcccgcgc gctggcgcca cgtcgaagtg ggcggtgccg
    12601 gccaccggat ccagaaggtg caggtagtag ggcagcacgc cggcatcgaa cagccgttga
    12661 ctcagctcgg ccaggtcatc ctcgttgtcg ttgactccct tgaggagcac ggactggttc
    12721 agaagctggg cgcccgcggc cttgaggtcc gccaacgccc gtcgcaccga ggcgtcgatc
    12781 tcgttggcat gattgcagtg cacgacgaaa accgtccgca agcgggttgc ggccagcgta
    12841 tcgaccaggc ccgcgtcgac ccgttcggga atgatgatgg ggaagcgggt gtggaaccgc
    12901 agggtttgga cgcttgcgac gttctccatg gcgcgaatca actcgccgat cgcctcgttg
    12961 gtcagcgtga gtggatcgcc gcctgaaagt atgacttccg tcacctgcgt gtccgcgctc
    13021 agcgcgtcga cggcgcccgt ccagcgttgg cgtgacgcaa gctggtccgc gtaggggaaa
    13081 ttcctgcgga agcagtagcg gcagtggacg ggacacgcgc cggaggcgat cagcagcgcg
    13141 cggccggaat actttcgcac gacgccggct cgggcaaagc gctgctcctg cagcgggtcg
    13201 gcttcgaagc ccggcgccgt gatgcgctct gccccatcgg ggagaacctg gcgcagcagg
    13261 gggtcgtggg gatctccctt gcgcatgcgt gccgcaaagg atctcggaac cagcatggga
    13321 aagtcatcgt cgtgaccgtc catcaccggc gcccaggccg ggtcgagctc cagggcctcg
    13381 atcagctccc ggggcgttct gatggcgttg cgcaacgcct cctgccagct gccggtcgcc
    13441 ggatcgtcgg cgaccgtgcg tgtccgcaga cgggccgcgg attgctcggg tgcactttga
    13501 gtcatggggt tcacgtccgg gacgagtatc gcgcaatcgt ctgcggtctc atagcgcagc
    13561 cggtcccggt cccgggtcgg ccgtcgactc aagaagagac ctcctccgtc ggccgccccc
    13621 gccggatcag gaggtagagg agcgcgcctc ctgcgatgat ggccaggccc cacaacccct
    13681 ctttcggtcg gtgggcgatg atgtagccga gggtccatcc cgtgatggcc aggtagacga
    13741 gcggcggtag cgggtaccac gtcgtgcgat aggggcgggg caggtcgggc tggcggatgc
    13801 gcagcacgaa caggcccagc accgtgaaga acgtgctgac cccgagggtg aagccggcaa
    13861 acacgagaat ggcttcgaaa gacgctgtga gaatgaaggc cagcgccagc acggactgaa
    13921 cgacgatggc cgtcaccggt accccgtcgc cgctcacccg agccagaaaa cgaaacgcgg
    13981 ggtagtcctg gccgatcacc tgcaaaaccc ttggacccgc catgatcatg gcgctggcgg
    14041 tggatatcag aagcagagcc agaatgacgg ccatgatggc cgcgccgttc gagccgaagg
    14101 cgtgctcggc ggcgatgtag ccgatctcca gcctgccctc catggcctcc gccggcgcga
    14161 cgtacagaaa ggttgcattc aaaagcacgt acagcagcat tacgaccacg gttccctgga
    14221 gcagcacccg ggagagtgtc tgctcggggc ggtccagctc gcccgtgagg taggtggccg
    14281 cgttccagcc ggtgtaggcg tagttgacgt agatcaaagc cacggcgaac gcgccgctgg
    14341 tgagcagcgc tccgtcaccc tcgtggggca gcagcgatat cccctgaggc tcgtcgacca
    14401 gcagggcggc gagcaggcag aaaccgagaa tcaggatgat cttcacggca gtgaacgccc
    14461 gctggacgcc gccgctcgag cgtcgcgagg tggcgtgagc aacggaaagc accacgacca
    14521 gaccgcacgc cagccagacc ggcgacagcg cgggaaagac cgaggccaga tagatgccga
    14581 aggtaatggc ggcgagcgcc gtgggtgcgg caaacccgat ggtggcggag atccagccgg
    14641 acacgaagcc tacggccgga tgatagattc gtcccaggaa gttgtactcg ccacccgagc
    14701 gcggcagagc cgcgccgagc tccgcgtagg acagcgcccc gcagagcgcc gtcaaccccc
    14761 ccaccgccca gagcatcagc aggacgaact cggatctgat gtcggccagc tgaaagccca
    14821 ggctcgtgaa cacgccggta ccgatcatgt tggccatgat cacggccgtg gctgtgcgcg
    14881 cgctgaattt catgagcccg gggcctcagg cacccgtcac ggtgagccgg tcgcgactag
    14941 cgccggtcca gacgatagat cttgccggta tcgacccagc cttcgtcatc gtcgacgtcg
    15001 tacttgttca ggtaggcctg agcgacgcgc ctgcgctcgg ccatctcggt gacggcagtc
    15061 gcgcgcagcg ggtagatgtc gccgttggtc ctgagcctta cgctcgggtc accgtcgatt
    15121 cgctccgtcc agttggtgcc gtcctcgccc gtggccacgt acacgtctgc gccgatcccg
    15181 acggaccaca cgttcacgga gtaggggtcg ttcggatccg tctctatctg cacggtatcg
    15241 atggcttcca gaaaggacca gtcggccggc ggcaccttct cgtcgccgct gagcgccccc
    15301 ccggcgaaga cgatgaacgg ctctgcacag ccgctcagga ggatgatgat tgcgcctgtg
    15361 agtgcgagtc gcgccatggc ctgaccttcg agaagacggc aattagggcg aattctttgc
    15421 cgcctgtaaa tagatttctc gccgtgatcg gcccacggac cgtatgatct gagggtccgg
    15481 tcgcttgcgg atgggagggc gtggacgtgc gcgcgatgca gcttacggag cccggtggag
    15541 atttccgcct ggtggacttg ccgaagcctc gacccgcggc gggcgaggtt ctactggccg
    15601 tgcacgcctg cggcgtctgc cgcaccgatc tccacgttgt ggacggggac atcgtcgacg
    15661 gccgctatcc cgtcgtgccc gggcaccaga tcgtcggggt ggtcgagcag gcgccggacg
    15721 gcgatctgca accgggacag cgggtgggcg tcccgtggct cggccatacc tgcggcacct
    15781 gcgttttctg ccttgcgggc caggagaacc tgtgcgattc ggccgaattc accggatacc
    15841 atcgccaggg tggattcgcg gagtacgcgg tcgccgacgc ccgcttctgt ttccccatcc
    15901 cggaaagcta tccggacgtt caggcggcgc cgctgctgtg cgcggggctg atcggctacc
    15961 gggccctggg aatggttggg gatgctcgaa agctgggttt ctacgggttc gggaatgccg
    16021 cccacatcct cacccagatt gcggtatggg aggggcgcga agtgtccgcg tttacccgcc
    16081 cgggggatgc ggccggccag cgattcgcga ggggtctggg tgctgtgtgg gcgggtgatg
    16141 cgtcgacgcc ccctccggag cccctggacg ccgccatcat cttcgcgccg gacggggcgc
    16201 tggtgccggc ggccttgaag catgtgcgca agggtggggt ggtggtctgc gcgggcattc
    16261 acatgtccga catcccttcg ttcccctacg ccgatctctg gggcgagcgc atcatccgct
    16321 cggtggccaa cctgacccgc gaagacggcg tgcggttgct ggaggtggcg ccccgcgtcc
    16381 cggtccgcac cgaggtgcag gttttcccgc tcgagcggac ggcggatgcg ctcgatgctc
    16441 tgcgggaagg ccgcgtcgag ggaggcgccg tcgtccaggt gcgctagagc gtgtccacca
    16501 gcttgcgcag ctgctcgcag ctgcgaatcc gctcttgtga cgttctgccc atgaagccca
    16561 cgttcagggc ggtcactcct gattcgctca gcgcttgcag gcggtctcga acgaaaccct
    16621 cgggcccgat gagcgagttg ttggccaggt agctctcggg gatagcggcc tcggc
//
LOCUS       DBNHLM010000013        12033 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1617598, whole genome shotgun sequence.
ACCESSION   DBNHLM010000013 DBNHLM010000000
VERSION     DBNHLM010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12033)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12033)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12033
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1617598"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="AC16QF_00705"
     CDS             <1..771
                     /locus_tag="AC16QF_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR6447098.1"
                     /translation="IDIDQRPIGRTPRSNPATYTGAFTPIRDWFAGLPEAKARGYKPG
                     RFSFNVKGGRCEACQGDGVIKVEMHFLPDIYVPCDVCKGKRYNRETLDVRYKGRNIHE
                     VLEMTVEEAREFFDAVPMLARKLQTLVDVGLSYIALGQSATTLSGGEAQRVKLSRELS
                     KRDTGKTLYILDEPTTGLHFHDIAQLLDVLHRLRDQGNTVVVIEHNLDVIKTADWVVD
                     LGPEGGSGGGRVVAAGTPEHLARKRGSHTGRFLKKMLG"
     gene            complement(789..1235)
                     /gene="rplQ"
                     /locus_tag="AC16QF_00710"
     CDS             complement(789..1235)
                     /gene="rplQ"
                     /locus_tag="AC16QF_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR6447099.1"
                     /translation="MRHRKSGRHLNRTSSHRQAMFRNMAASLIEHEVIKTTLPKAKEL
                     RRVAEPLITLAKEDSVANRRLAFARTRSDAAVGKLFVELGPRYKDRPGGYTRILKCGN
                     RAGDAAPMAFIELVDRPVVDEFDEDEFDAADEQADEAEELEAEEKG"
     gene            complement(1339..2334)
                     /locus_tag="AC16QF_00715"
     CDS             complement(1339..2334)
                     /locus_tag="AC16QF_00715"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR6447100.1"
                     /translation="MAQSVTEFLTPRHIDIEASSPTRAKVTLEPLERGFGHTLGNTLR
                     RILLSSMPGCAITEVQIDGVLHEYSTLEGVQEDVINILLNLKGIAVILHSQDEAVITL
                     NKDGAGQVTAGDFQLTQDVEIANPDHLICSLNDNGAIRLEARVTRGRGYVPADSREEE
                     EDSRPIGVLSLDATFSPIRRVAYGVESARVEQRTDLDKLVLDLETNGTIEPEEAIRRA
                     ATILQQQLAVFVDLESEGEPVAEEKEDEIDPILIRPVDDLELTVRSANCLKAENIYYI
                     GDLIQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASLRGSDKVA"
     gene            complement(2382..3002)
                     /gene="rpsD"
                     /locus_tag="AC16QF_00720"
     CDS             complement(2382..3002)
                     /gene="rpsD"
                     /locus_tag="AC16QF_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR6447101.1"
                     /translation="MARYIGPKLKLSRREGTDLFLKSGVRPIDSKCKLEQPPGQHGAR
                     RGRLSDYAIQLREKQKVRRLYGVLEKQFRNYYKKAERQKGATGENLLRLLECRLDNVV
                     YRMGFGATRAESRQLVSHKAITVNGSLVNIPSYQVAADDVVGVYEKARGQLRIQSAMQ
                     LASQRAPVEWVDVDPTKLEGVYRRPPDREELPPEINENLIVELYSK"
     gene            complement(3027..3401)
                     /gene="rpsK"
                     /locus_tag="AC16QF_00725"
     CDS             complement(3027..3401)
                     /gene="rpsK"
                     /locus_tag="AC16QF_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR6447102.1"
                     /translation="MAEKKKAKRVVIDGVAHVHASFNNTIITISDRQGNALCWATAGG
                     SGFRGSRKSTPFAAQVAAERVSNTALEHGMKSVDVYIKGPGPGRESAVRALNAAGLRI
                     NSIADVTPIPHNGCRPPKRRRV"
     gene            complement(3404..3760)
                     /gene="rpsM"
                     /locus_tag="AC16QF_00730"
     CDS             complement(3404..3760)
                     /gene="rpsM"
                     /locus_tag="AC16QF_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR6447103.1"
                     /translation="MARIAGINIPDNKHAVVSLTYIFGIGRTTAVKICEAAGVNPSSK
                     IQDLDEANLDAIRAEVAKFTVEGDLRRAVSMNVKRLMDLGCYRGIRHRRGLPVRGQRT
                     RTNARTRKGPRRPIRK"
     gene            complement(3775..3891)
                     /gene="rpmJ"
                     /locus_tag="AC16QF_00735"
     CDS             complement(3775..3891)
                     /gene="rpmJ"
                     /locus_tag="AC16QF_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR6447104.1"
                     /translation="MKVRASVKRMCRNCKIIKRNGVVRVICSAEPRHKQRQG"
     gene            complement(3901..5232)
                     /gene="secY"
                     /locus_tag="AC16QF_00740"
     CDS             complement(3901..5232)
                     /gene="secY"
                     /locus_tag="AC16QF_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014869934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR6447105.1"
                     /translation="MATNAGQLTGVQAGLSELRSRLLFVLIALLVYRMGTHIPVPGIN
                     PDRLAALFDQQQGGILDLFNMFSGGALERMSILALGVMPYITSSIIMNLMTMMYPSLQ
                     QLRKEGESGRRKITQYTRYGCLLIALIQGTSLSVTLANQGLAFSANFAFFFVATTTLV
                     TGALFMMWLGEQITERGVGNGISLIIFSGIVSGFPAAIGQSFEAARQGDINIIVLLFI
                     AALAVAIVGFVVFVERGQRRITVNYAKRQQGRRVFQGQSSHLPLKINMAGVIPAIFAS
                     SLLLAPASMAQWFGNSPGMDWLQQIALVLSPGQPLYILMFAAGIIFFSFFYTAIMFDP
                     KDMADNLKRQGAYVPGIRPGQQTARYIDDVITRLTVFGALYVTMVCLLPEFMIVAFNV
                     TFQLGGTALLIVVVVAMDFMSQVQAHLMSSQYAKLMEKSNLKGYGRSGRPR"
     gene            complement(5232..5666)
                     /gene="rplO"
                     /locus_tag="AC16QF_00745"
     CDS             complement(5232..5666)
                     /gene="rplO"
                     /locus_tag="AC16QF_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR6447106.1"
                     /translation="MRPNDISPASGSKPGKRRVGRGPGSGWGKTAARGQKGQKARSGG
                     SVPAWFEGGQLPLQMRVPKFGFRSRVGMTTAEVRLGEIGKVEGDVVDLDALKAANVLS
                     RNVKRARIMQSGRVERPLTVKGLIVTKGARSAIEAAGGKVED"
     gene            complement(5669..5854)
                     /gene="rpmD"
                     /locus_tag="AC16QF_00750"
     CDS             complement(5669..5854)
                     /gene="rpmD"
                     /locus_tag="AC16QF_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR6447107.1"
                     /translation="MSGNSIRVTLVKSPAGRLKKHRACVAGLGLRRIGHSVEVEDTPA
                     VRGMINRVSYMVRVEGE"
     gene            complement(5892..6398)
                     /gene="rpsE"
                     /locus_tag="AC16QF_00755"
     CDS             complement(5892..6398)
                     /gene="rpsE"
                     /locus_tag="AC16QF_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR6447108.1"
                     /translation="MAYTDEVREEGLVEKLVQVNRVAKVVKGGRIFGFTALTVVGDGN
                     GRVGFGRGKAREVPMAIQKAMEAARRNMVDVDLNGSTIWYAVTERHGASKVYMQPASE
                     GTGVIAGGTMRALLEAAGVHNVLAKCYGSTNPVNVIRATFKALTSVHSPDAVAAKRGK
                     TLEEIGAA"
     gene            complement(6401..6751)
                     /gene="rplR"
                     /locus_tag="AC16QF_00760"
     CDS             complement(6401..6751)
                     /gene="rplR"
                     /locus_tag="AC16QF_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR6447109.1"
                     /translation="MNVKKTARLRRARRGRMNMRERAAVRLTVNRTPRHIYAQVISGS
                     GDRVLAAASTLEQSLRSGNTGNVDAAAQVGKLIAERAKSAGISQVAFDRSGYKYHGRV
                     RALAEAAREGGLEF"
     gene            complement(6785..7318)
                     /gene="rplF"
                     /locus_tag="AC16QF_00765"
     CDS             complement(6785..7318)
                     /gene="rplF"
                     /locus_tag="AC16QF_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003293037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR6447110.1"
                     /translation="MSRVANSPIAVPPGVEVKLDGQHIRVKGSKGELDMNVHQNVEIT
                     QNDGALQVAARSPEKQSRAMAGTTRALVFNMVTGVSTGFERRLQLQGVGYRAQAQGNK
                     LNLQLGFSHPVEYELPEGISVETPSQTEIVVTGADKQQVGQVSSEIRAFRPPEPYKGK
                     GVRYANERVRRKEAKKK"
     gene            complement(7330..7722)
                     /gene="rpsH"
                     /locus_tag="AC16QF_00770"
     CDS             complement(7330..7722)
                     /gene="rpsH"
                     /locus_tag="AC16QF_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR6447111.1"
                     /translation="MTMQDPIADLLTRIRNAQRASHESVEIPCSKIKAAVCEVLKEEG
                     YIGDFSVDDAAKPTLNIGLRYHEGRPVIEEIKRISRPGLRIYKACDELPKVRGGLGVA
                     IVSTNKGVMTDRQARAMGVGGEVLCTVF"
     gene            complement(7734..8039)
                     /gene="rpsN"
                     /locus_tag="AC16QF_00775"
     CDS             complement(7734..8039)
                     /gene="rpsN"
                     /locus_tag="AC16QF_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HIR6447112.1"
                     /translation="MAKVSIIEREKKRSRMVAKYAAKRAALKAIINNREASDEERWEA
                     QLKLQRLPRNASPVRLQRRCGITGRPHGVYRKFGLGRNKLREAAMRGDVPGLVKASW"
     gene            complement(8058..8597)
                     /gene="rplE"
                     /locus_tag="AC16QF_00780"
     CDS             complement(8058..8597)
                     /gene="rplE"
                     /locus_tag="AC16QF_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR6447113.1"
                     /translation="MADLYRRYMDEIRPRLQEELGISNIMRVPRLEKITLNMGVGEAV
                     GDRKIMDSAVGDLTVISGQKPIVTNARKSEAAFKIRTGWPVGCKVTLRRARMFEFLER
                     LIGIAIPRMRDFRGLNPRSFDGRGNFSMGITEQIVFPEIDYDKIDKIRGLDITVTTTA
                     KTDDEGRALLRAFDFPFRN"
     gene            complement(8603..8917)
                     /gene="rplX"
                     /locus_tag="AC16QF_00785"
     CDS             complement(8603..8917)
                     /gene="rplX"
                     /locus_tag="AC16QF_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR6447114.1"
                     /translation="MLKIKKDDEVVVISGRDKGKRGGVLRVLADGRLLVGGVNMVKKH
                     QRPNPNIGQQGGIVDQEAPIEVSNVAIWNPDEERADRVGFREEDGRKVRVFKSTGKVI
                     EE"
     gene            complement(8921..9289)
                     /gene="rplN"
                     /locus_tag="AC16QF_00790"
     CDS             complement(8921..9289)
                     /gene="rplN"
                     /locus_tag="AC16QF_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR6447115.1"
                     /translation="MIQTETMLDIADNSGARRVQCIKVLGGSHRRYAGIGDIIKVTVK
                     EAIPRGRVRKGQVMNAVVVRTRKGVRRPDGSIIRFDQNAAVLLNQQNQPVGTRIFGPV
                     TRELRTENFMRIISLAPEVL"
     gene            complement(9329..9613)
                     /gene="rpsQ"
                     /locus_tag="AC16QF_00795"
     CDS             complement(9329..9613)
                     /gene="rpsQ"
                     /locus_tag="AC16QF_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR6447116.1"
                     /translation="MADEQTAQPQESVTRSVTGTVVSDRMDKTITVLIERKVQHPVYG
                     KIVRRSSKLHAHDENNESRTGDTVMVVECRPLSKTKSWMLHSIVERAREV"
     gene            complement(9606..9797)
                     /gene="rpmC"
                     /locus_tag="AC16QF_00800"
     CDS             complement(9606..9797)
                     /gene="rpmC"
                     /locus_tag="AC16QF_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR6447117.1"
                     /translation="MSASELREKSPQELREELLRLRKEQFHLRMQKASGQLGQSHLLT
                     ETRREIARVKTVMQEKQDG"
     gene            complement(9800..10213)
                     /gene="rplP"
                     /locus_tag="AC16QF_00805"
     CDS             complement(9800..10213)
                     /gene="rplP"
                     /locus_tag="AC16QF_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR6447118.1"
                     /translation="MLQPKRTKFRKQQKGRNRGLAHRGSSVSFGEFGLKAVGRGRLTA
                     RQIEAGRRAMTRRIRRGGKIWIRVFPDKPITKKPLEVRQGKGKGAVEYWVSQIQPGRV
                     LYEMEGVPEDIAREAFALAAAKMPFATAFVKRTVM"
     gene            complement(10233..10892)
                     /gene="rpsC"
                     /locus_tag="AC16QF_00810"
     CDS             complement(10233..10892)
                     /gene="rpsC"
                     /locus_tag="AC16QF_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003176422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR6447119.1"
                     /translation="MGQKVHPTGIRLGIVKDHNSVWYADRKSYSDYLLNDLEVRDYLR
                     KRLSQASISRIEIERPSQTARVTIHTARPGIVIGKKGEDVERLRGELTVRMGVPVHIN
                     IEEIRKPELDALLVAQNVAQQLERRVQFRRAMRRVIQNAMRLGAEGIKVRVAGRLGGA
                     EIARSEVYHEGRVPLHTLRADIDYATWEAKTTYGIIGIKVWIFKGEIIGPSGESAAAQ
                     A"
     gene            complement(10900..11232)
                     /gene="rplV"
                     /locus_tag="AC16QF_00815"
     CDS             complement(10900..11232)
                     /gene="rplV"
                     /locus_tag="AC16QF_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003304070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR6447120.1"
                     /translation="MRVSAKANRLRIAPQKARLVVDQVRGKPVSDALDILGFSTQKGA
                     VLVRKVVESAIANAENNEGADVDELKISEIFVNEGLTMKRIRPRAKGRADRIFKRTSH
                     ITVTVTDE"
     gene            complement(11253..11528)
                     /gene="rpsS"
                     /locus_tag="AC16QF_00820"
     CDS             complement(11253..11528)
                     /gene="rpsS"
                     /locus_tag="AC16QF_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR6447121.1"
                     /translation="MPRSLHKGPFVDLHLMQKVETAVAKNEKRPIRTWSRRSMVVPDM
                     VGLTIAVHNGRQHVPVFINEDMVGHKLGEFAPTRTYRGHAADRKARR"
     gene            complement(11532..>12033)
                     /gene="rplB"
                     /locus_tag="AC16QF_00825"
     CDS             complement(11532..>12033)
                     /gene="rplB"
                     /locus_tag="AC16QF_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR6447122.1"
                     /translation="VSAGDELRSGTGAPIRAGNAMALRSVPVGSVVHCIELKPGKGAQ
                     MARSAGTSAQLVAREGAYATLRLRSGEMRRVLADCRATLGEVGNSEHNLRSLGKAGAK
                     RWRGVRPTVRGVAMNPVDHPHGGGEGRTSGGRHPVSPWGVPTKGHKTRSNKRTDKLIV
                     RRRGRR"
BASE COUNT         2050 a         4005 c         3503 g         2475 t
ORIGIN      
        1 atcgacatcg accagcgacc tattggtcgt acaccgcgtt cgaatccagc gacttacacc
       61 ggggccttca cccctatccg cgactggttc gcgggcctgc ccgaggccaa ggcgcgcggt
      121 tacaaacccg ggcgtttcag cttcaacgtc aagggcgggc gctgcgaggc gtgtcagggt
      181 gacggcgtca tcaaggtgga gatgcacttc cttcccgaca tatacgtgcc atgcgacgtc
      241 tgcaaaggca aacgctacaa ccgggagacg ctcgacgtcc gctacaaagg caggaacatc
      301 cacgaggtcc tggagatgac cgtggaggaa gcccgggaat ttttcgacgc ggttcccatg
      361 cttgcacgca agcttcagac cctggtggac gtggggctgt catacatcgc cctcggacag
      421 agcgcgacga cgctttcagg cggcgaggcg caacgcgtca agctgtccag ggagctttcc
      481 aagcgggaca ccggcaagac cctgtacatc ctggacgagc ccactacggg ccttcacttc
      541 cacgacatcg cccagctgct ggacgtgctg caccgtctgc gcgatcaggg aaatacggtg
      601 gtcgtgatcg agcacaacct ggacgtcatc aagacggccg actgggtggt ggacctgggg
      661 cccgagggcg gctctggcgg cggccgggtg gtggccgcag ggaccccgga gcatctcgcc
      721 agaaagcgtg gctctcacac cgggcgtttt ctcaagaaga tgctggggta ggtgccttcg
      781 cgccaggctc agcccttctc ctcggcttcc agctcttctg cttcgtccgc ctgctcgtcc
      841 gccgcatcga actcgtcttc gtcgaactcg tcgaccacgg gacgatccac cagctcgatg
      901 aacgccatgg gcgccgcatc gcccgcccga ttgccgcatt tcaggatgcg ggtgtagccg
      961 cccgggcgat ctttgtagcg cggtcccaac tctacgaaca gcttacccac cgccgcgtcg
     1021 ctgcgcgtgc gcgcgaatgc caggcgccgg ttggcgacgc tgtcttcctt cgcgagggtg
     1081 atgagaggct cggcgacgcg ccggagctct ttcgctttgg ggagcgtcgt cttgatgacc
     1141 tcgtgctcaa tgagcgacgc ggccatgtta cggaacatag cctgccggtg tgagctcgtg
     1201 cgattgagat gtcgtccact cttgcgatgt cgcatgactg cttcctaagt tactccgcgc
     1261 ctttgccgcg acgacggccc gagatcacca cctccggcct tcctcgacct cttcaggcga
     1321 cctcttcagg cgacctcttc aggcgacctt gtcgctgcct ctgagactgg ccggaggcca
     1381 gttctccagg cgcatgccaa gggacagacc gcgagacgcc aggacgtcct tgatctcggt
     1441 cagtgatttc ttaccgagat tgggggtctt gagcagctca acctcggtgc gctgaatgag
     1501 gtcgccgatg tagtagatgt tttccgcctt gagacagttg gccgagcgca ccgtgagctc
     1561 gagatcgtcc acgggtcgga tgaggatcgg gtcgatctcg tcttccttct cctccgcgac
     1621 aggctcgccc tcgctctcca ggtccacgaa caccgccagc tgctgctgga gaatggtcgc
     1681 cgcccggcgg atcgcctcct cgggctctat ggtcccgttg gtctccagat ccagcacgag
     1741 cttgtccagg tcggttcgct gctcgacccg ggcgctctcg acgccgtagg cgacccgacg
     1801 aatcgggctg aaggtagcgt cgaggctgag cacaccgatc ggccgggagt cctcttcctc
     1861 ttcacggctg tcggccggca cgtagccccg accgcgcgtg acacgggctt ccaggcgaat
     1921 ggcgccgttg tcgtttagag agcagatcaa gtgatccgga ttcgcgatct ccacgtcctg
     1981 agtcagctgg aagtcaccgg ccgtcacctg gccagcgccg tccttgttca gggtgattac
     2041 cgcctcgtcc tggctgtgga gaatcacagc gatccctttc aggttgagca ggatgttgat
     2101 gacgtcttcc tgcacgcctt cgagggtgct gtactcgtgc agcacgccgt cgatctgcac
     2161 ctcggtgatc gcgcagcccg gcatggatga cagcagaatg cgccgcagcg tgttgcccag
     2221 cgtgtgaccg aagccccgct cgagaggctc cagcgtcacc ttcgcccggg tcgggctgct
     2281 ggcctctatg tcgatgtggc gtggggtcag aaattcggtt accgactgtg ccatatgttt
     2341 cgtctcctga tatctggagt gttcagttac tcttcgggcc cttacttgga gtagagctcc
     2401 acgatgaggt tctcgttgat ctccggcggc agctcttccc gatccggcgg gcgcctgtac
     2461 acgccctcga gcttggtcgg gtccacgtcc acccattcca ccggcgcccg ctggctggcc
     2521 agctgcatag ccgactggat gcgcagctgc ccgcgtgcct tttcatacac gccgaccacg
     2581 tcatcggcgg ccacctggta ggaggggatg ttgaccagcg aaccgttcac cgtgatggcc
     2641 ttgtgcgaga ccagctgccg tgattccgct ctggtcgccc cgaagcccat gcgatacacc
     2701 acgttgtcca ggcggcattc caaaagcctg agcaggtttt cgccggtggc gcccttctgt
     2761 cgttccgcct tcttgtagta gttgcggaac tgcttctcga ggacgccgta aaggcgtctg
     2821 actttctgct tttcacgcag ctggatcgcg tagtcggaca acctgccgcg ccgagcacca
     2881 tgctgaccgg gaggctgctc gagcttgcac ttcgagtcga tggggcgtac gccgctcttg
     2941 agaaacaggt cggtgccctc gcggcgcgaa agcttgagtt ttggtcctat gtatcgagcc
     3001 atggtcgtct atctctccct tgctcgtcag acacgacgcc gcttcggcgg gcggcagccg
     3061 ttgtgaggaa tgggggtcac atcagcgatg ctgttgattc gaaggcccgc cgcgttgaga
     3121 gcgcgaacgg cggactcgcg gcccggtccc ggacccttga tgtagacatc cacgcttttc
     3181 atcccgtgct cgagcgccgt gttggatacg cgctcggccg cgacctgggc agcaaacggc
     3241 gtgctcttgc gagagccgcg gaagcccgag ccgcccgccg tcgcccagca cagcgcattg
     3301 ccctgccggt cgctgatggt gatgatggtg ttgttgaacg acgcgtgcac gtgcgccacg
     3361 ccgtcgatca ccacccgctt cgcctttttc ttctcagcca tatctatttc ctgattggac
     3421 gacggggacc tttgcgagtg cgcgcgttgg tgcgcgttct ctgaccccga accggcagcc
     3481 cgcgccggtg gcgaatcccg cggtagcaac ccagatccat caaccgcttg acgttcatgg
     3541 acacggcacg ccgcaggtcg ccttccacgg tgaacttcgc cacctctgcc ctgatggcgt
     3601 cgagattggc ctcgtccagg tcctgaatct tgctcgaggg gttcaccccg gccgcctcgc
     3661 agatcttcac cgccgtggtg cgaccgatcc cgaaaatata ggtcagcgat acgaccgcgt
     3721 gcttgttgtc cggtatgttg attccggcaa ttcgtgccat tcaaatgctc ctagtcagcc
     3781 ctgccgctgc ttgtgccgcg gctcggcgct gcagatcacc cgaaccacac cattgcgctt
     3841 gatgatcttg cagtttcgac acatccgctt aaccgatgct cttactttca taacgactcc
     3901 ctacctcggc cggcccgatc ggccatagcc cttgaggtta gatttctcca tgagcttcgc
     3961 gtactggctc gacatcagat gcgcctgcac ctgagacatg aaatccatgg cgaccaccac
     4021 cacgatgagc agggcggtac cgccaagctg aaacgtgacg ttgaaggcca cgatcatgaa
     4081 ctccggcagc agacagacca tcgtcacgta aagcgccccg aacacggtca agcgggtgat
     4141 cacgtcatca atgtagcgag ccgtctgctg gcccggacgg attccgggaa cgtaagcgcc
     4201 ctgccgcttg aggttgtccg ccatgtcctt cggatcgaac atgatcgcgg tgtagaagaa
     4261 actgaagaaa atgatgcccg ccgcgaacat caggatatac agcggctgcc ctggcgagag
     4321 cacgagggcg atctgctgga gccagtccat tcccgggctg tttccgaacc actgggccat
     4381 ggaggccggc gccagcagaa ggctcgaagc aaagatggcg gggatcacgc ccgccatgtt
     4441 gatcttcaga ggcaggtggc tgctctggcc ctggaacacc cggcgaccct gctgtcgctt
     4501 ggcgtagttc actgtgatcc ggcgctggcc gcgctcgacg aacacgacga aaccgacgat
     4561 ggccaccgcc aatgccgcga tgaacagcag cacgatgatg ttgatgtcgc cctgccgcgc
     4621 ggcttcgaaa gactgcccga tggcggccgg aaatccggag acgattcccg agaagatgat
     4681 gagagagatg ccgttaccga ccccgcgttc cgtgatctgc tcgccgagcc acatcatgaa
     4741 caacgcgccg gtgaccagcg tcgtggtcgc gacgaagaag aaggcgaagt tagcggagaa
     4801 agccaggccc tgattcgcca gagtgaccga aagagaggtg ccctgaatga gcgcgatcag
     4861 caggcagccg tagcgggtgt actgggtgat cttgcgccgt cccgactccc cctcctttcg
     4921 cagctgctgg aggctcgggt acatcatggt catcagattc atgatgatgc tggaggtgat
     4981 gtaaggcatc acacccagcg cgagtatgct catccgctcc agcgcgccgc ccgagaacat
     5041 gttgaaaaga tccagaatgc cgccctgctg ctgatcgaac agcgcagcca ggcgatccgg
     5101 attgatgccg ggaaccggga tgtgggtccc catgcgataa accaggagcg cgatcaggac
     5161 gaacagcagc cgggagcgaa gctcgctcaa gcccgcctgc acgcccgtga gttgacccgc
     5221 gttggtcgcc atcagtcttc taccttgccc ccggccgctt cgatggcgct gcgcgcgcct
     5281 ttggtcacga tcaaaccctt gacggtaagc ggtcgctcca cgcgcccgga ctgcatgatt
     5341 cgcgcccgct tgacgttccg cgacagcacg ttggccgcct tcaacgcgtc gaggtcgacc
     5401 acgtcgcctt cgactttacc tatctcgccc agtcgcacct ccgcggtggt cataccgacc
     5461 cgcgagcgga agccgaactt cggaacccgc atctggagcg gcagctgacc accttcgaac
     5521 caggccggaa cgcttccgcc ggaccgggct ttctggcctt tctggccccg cgcggccgtt
     5581 ttgccccagc cggatccggg gccacggccc acccgccttt tacccggctt gctgcccgac
     5641 gcgggagaga tgtcattcgg ccgcatcgtt actctccttc aacccggacc atgtagctga
     5701 ctcgattaat catgccgcgc accgccggtg tgtcctccac ctcgacgctg tgcccgatgc
     5761 gccgcaggcc aaggccagcg acgcaggctc ggtgcttttt cagccgaccc gcagggcttt
     5821 tgaccaacgt gactcgaatc gaattaccgc tcatgaccac aacttcctca aactgccttt
     5881 gccgccccgc ttcaggccgc gccgatctcc tcgagagtct tgccccgttt cgcggccacg
     5941 gcgtccgggg aatgaacact cgtgagcgct ttgaaggtcg ctcgaatcac gttcaccgga
     6001 ttcgtagacc cgtagcactt ggcgagcacg ttgtgcacgc cggcggcttc tagaagcgcc
     6061 ctcatggtgc cgccggcaat gacgccggtg ccttccgatg caggctgcat gtaaaccttg
     6121 gacgcgccgt ggcgctcagt gaccgcgtac cagatggtcg agccgttgag gtctacgtcc
     6181 accatgttgc ggcgcgccgc ttccatggct ttctgaatcg ccatgggcac ttcccgcgct
     6241 ttgccgcgcc cgaagcccac gcgtccattg ccgtcaccga cgaccgtcag cgcggtgaac
     6301 ccgaatatgc ggccgccttt gacaaccttg gcaacgcgat tgacctggac cagcttttcg
     6361 accaggccct cttcacgcac ttcgtccgta tacgccatag ttagaactcc aagccgcctt
     6421 cgcgcgccgc ttcagcaagc gccctcacgc ggccgtgata cttgtaaccg gagcgatcga
     6481 aggccacctg gctgatgccc gccgacttcg cgcgctccgc gatgagtttg cccacttgtg
     6541 ctgcagcgtc gacattgccc gtgttgccgc tgcgcagcga ctgctccaac gtggacgccg
     6601 ccgccagcac ccgatcgcca gagccagaga tcacctgggc ataaatgtgc ctgggcgtgc
     6661 gattgacggt cagacgcacc gctgcgcgtt cgcgcatgtt catcctgccg cggcgcgcgc
     6721 gcctcaatcg ggccgttttc ttaacgttca tgcaaactca acctgttcag aaccctgttc
     6781 cgcgctactt cttcttggct tccttgcgcc gcacccgctc gttcgcatac cgaacgccct
     6841 tgcccttgta tggctccggc ggtcggaagg cgcgaatctc ggaggaaacc tggcccacct
     6901 gctgcttatc cgcacccgtg acgacgattt ccgtctggct gggggtctcc acggagatcc
     6961 cctcgggaag ctcgtactca accggatggg agaagccgag ctgcagattc agcttgttgc
     7021 cctgagcctg cgctctgtaa cccaccccct ggagctgcag ccggcgttcg aagcccgtgg
     7081 acactccagt caccatgttg aacacgagcg cacgcgtggt gcccgccatt gcccgggact
     7141 gcttttcagg cgaccgcgcg gcgacctgga gagccccgtc gttctgggtg atctccacat
     7201 tctggtggac attcatgtcg agctcgccct tggagccctt caccctgatg tgctgcccat
     7261 ccaacttcac ctcgacacct gggggaaccg cgatgggact gttcgctact cgggacatcg
     7321 cctacctcgt caaaatacgg tgcacagcac ttcgccgccc acacccatgg cccgagcctg
     7381 ccggtcggtc atgacaccct tgttggtgct cacgatcgcc acgccaagcc cgccacgcac
     7441 cttaggcagc tcatcgcacg ccttgtagat gcgcaaaccg gggcggctga tccgcttgat
     7501 ttcctctatc acgggcctgc cttcgtgata tcgcaaaccg atattgagcg tcggcttggc
     7561 cgcatcgtcg acgctgaagt ccccgatgta gccctcttcc ttgagcacct cgcagaccgc
     7621 ggcctttatc ttggagcagg gaatttcaac cgactcgtgg ctggcacgct gggcgttgcg
     7681 aatacgcgtc aacaggtcag caatggggtc ttgcatcgtc atggtcgcac ctcttaccag
     7741 ctcgccttga ccaggcccgg aacgtcgcca cgcattgccg cctcgcgcag cttgttccgg
     7801 ccgagaccga acttgcggta cacgccatgg gggcgaccgg tgatcccgca acgacgctgc
     7861 agccgcacgg ggctggcgtt gcggggcaga cgctgcagct tcagctgcgc ttcccaacgc
     7921 tcctcgtcgg acgcctcacg gttgttgatg atcgctttca gagcggcgcg cttggcagcg
     7981 tacttggcca ccatgcggct gcgcttcttc tcacgctcaa ttatcgaaac ctttgccata
     8041 actcgagcta tcccaaatca gtttctgaac ggaaaatcaa aggctcgcag aagcgcgcga
     8101 ccttcatcgt ccgtctttgc agtagtggtg accgtgatgt cgagaccacg gattttgtcg
     8161 atcttgtcgt agtcgatctc cgggaagacg atctgctccg taatccccat ggagaagtta
     8221 ccccggccgt cgaaagaccg cgggttgagt ccccggaagt cgcgcatgcg cgggatcgcg
     8281 atcccgatca gccgctccag gaactcgaac attctggcgc gacgcagcgt caccttgcag
     8341 cccacgggcc agccggttcg aatcttgaac gccgcttcgg atttccgggc gttggtgacg
     8401 atgggcttct ggcctgaaat caccgtgaga tcgccaaccg cgctgtccat gatcttgcga
     8461 tcacccacgg cctctcccac ccccatgttc agggtgatct tttccagccg cgggacccgc
     8521 atgatgttgg agatacccag ctcctcctgc agcctcggcc ggatctcgtc catatagcgt
     8581 cgatacaagt cagccattgc ccttactctt cgatgacctt gccggttgat ttgaatacgc
     8641 gtaccttgcg gccgtcctcc tcgcgaaagc ccacccgatc agcgcgctcc tcgtccggat
     8701 tccagatcgc gacgttggat acctcgatgg gagcttcctg gtcgacgatg ccgccctgct
     8761 ggccgatgtt cgggtttggt ctctgatgct tcttgaccat gttgaccccg ccgacgagca
     8821 accgaccgtc agcgagcacg cgcagcacgc cgccccgctt tcccttgtcc cggccgctga
     8881 tgacaacgac ctcatcgtct ttcttgatct tcagcattac ctagagcacc tccggagcga
     8941 gcgagatgat ccgcatgaag ttctcggttc gcagctcccg agtgaccggc ccgaaaatcc
     9001 gggttccaac gggctgattc tgctggttta gcagcaccgc cgcgttctgg tcgaagcgga
     9061 taatcgatcc gtccgggcgc cgcacaccct tgcgggtgcg gaccaccacg gcgttcatca
     9121 cctgaccttt gcgcacccgc ccgcgcggaa tggcttcctt caccgtaacc ttgatgatgt
     9181 cgccgatccc cgcatagcgg cgatgggacc cgcccagcac cttgatgcac tggacgcggc
     9241 gcgcgccgct gttgtcagcg atatccagca tggtttcagt ctgaatcatc ggttacaccc
     9301 ttaatctcat gagctgaagg tctcagcttc aaacctcgcg cgcacgctca acgatgctgt
     9361 gcagcatcca ggatttggtt ttggacaacg gccggcactc tacgaccatc accgtgtcgc
     9421 ccgtacgtga ctcgttgttc tcgtcatggg catgcagctt ggatgatcga cgcacgatct
     9481 tgccgtacac cgggtgctgg acctttcgct cgatcagcac ggttatcgtc ttgtccatgc
     9541 ggtcgctgac cacggtcccg gtaaccgacc gggtaaccga ctcttgcggc tgagccgttt
     9601 gctcgtcagc catcttgctt ctcctgcatg accgttttca cacgtgcgat ctcgcggcgg
     9661 gtctccgtca gcagatgact ctggccgagc tggccgctgg ccttctgcat ccgcagatga
     9721 aactgctcct tgcgcagacg cagaagctcc tcgcgcagct cctgcggtga tttttctcga
     9781 agctcgctgg cactcatctc tacatcaccg tccttttaac gaatgccgtg gcgaacggca
     9841 tcttcgctgc cgcgagggca aaggcctctc tcgcgatgtc ctcgggtaca ccttccatct
     9901 cgtagagcac gcggccgggc tgaatctggg acacccagta ctcgacagcg cccttgcctt
     9961 tgccctggcg gacttccagg ggcttcttgg tgatcggctt gtcgggaaac acgcggatcc
    10021 agatcttgcc gccccggcgg atacgtcgcg tcatggcccg tcgaccggct tcgatctggc
    10081 gcgccgtcaa gcgcccgcga ccgacggctt tgaggccgaa ctcgccgaag ctcacgctgc
    10141 tgccgcgatg cgcaagtccg cgattgcgcc ctttctgctg ctttctgaat ttggttctct
    10201 ttggctgtaa cacttgctaa accttattcg cgtcaggcct gcgccgccgc ggactcgccc
    10261 gacgggccaa tgatctcgcc cttgaaaatc cagaccttga tgccgatgat cccgtaggtg
    10321 gtcttcgcct cccacgtggc gtagtcgatg tccgcacgca acgtgtgcag aggcacccga
    10381 ccttcgtgat agacctcgga gcgggcaatc tcagcgccgc ccaggcgccc ggcaaccctg
    10441 accttgattc cctcggctcc caggcgcatg gcgttctgaa tgacgcggcg catggctcgc
    10501 ctgaactgaa cccgacgctc gagctgctga gcgacgttct gagcgaccaa aagcgcatcc
    10561 agctccggct tccggatctc ctcgatgttg atgtgtaccg gcacgcccat gcgaacggtc
    10621 agctcaccac gcaggcgctc cacgtcctcg cccttcttgc cgatcacgat tccggggcgg
    10681 gccgtgtgaa tggttacgcg cgcggtctgc gacggccgtt cgatctcgat tcggctgatc
    10741 gaagcctggg agagacgctt gcgcagatag tcgcgcacct cgaggtcgtt gagcaggtaa
    10801 tccgagtagc tctttctgtc ggcataccaa acggaattgt ggtccttgac gatgccaagc
    10861 cgtatgccgg taggatgtac tttctgaccc atagctaacc tattcgtcgg tcaccgtcac
    10921 ggtgatgtga ctggtgcgtt tgaagatgcg atccgcccgc cccttggcgc gcggcctgat
    10981 ccgcttcatg gtgagccctt cgttgacgaa gatctcggag atcttcagct cgtccacgtc
    11041 ggccccctcg ttgttctcgg catttgctat ggcggactcg accaccttgc gtaccagcac
    11101 tgcgcccttc tgcgtgctga aacccaggat gtcgagagca tcgctgaccg gcttgccacg
    11161 gacctggtcc acgaccaggc gcgccttctg gggggcaatc cgcagccgat ttgctttcgc
    11221 gctgactcgc atgttccgct ctcctgacct ggctaccgcc gcgccttgcg gtccgcagca
    11281 tggccgcgat aggtgcgggt gggcgcaaat tcgccgagct tgtggcccac catgtcctcg
    11341 ttgatgaata ccggcacgtg ctgacgcccg ttgtgcaccg cgatggtcaa ccccaccatg
    11401 tccggcacga ccatggaacg ccgcgaccac gtgcgaatgg gccgcttttc gtttttggcc
    11461 accgccgttt ccaccttctg catgagatgg agatcgacga aaggtccttt gtgcagtgat
    11521 ctgggcacga cctacctcct gcctcggcga cgcacgatca gcttgtccgt ccgtttgttg
    11581 ctgcgggttt tgtggccttt tgtcggcacg ccccagggcg agacgggatg acgtccacct
    11641 gaagtgcggc cctcaccgcc accgtgcgga tggtccaccg ggttcatggc cacgccacgc
    11701 accgtggggc gcaccccgcg ccagcgtttg gcgcccgcct tgccgagcga acgtagattg
    11761 tgctcgctgt tgcctacttc tccgagcgtc gcccggcagt cggcgagcac acgccgcatc
    11821 tctccggagc ggagtcgaag cgtggcgtac gctccttccc gcgccaccag ctgcgcagac
    11881 gttcccgcgc tgcgggccat ctgtgcgcct ttgccgggct ttaactcaat gcaatgcacg
    11941 acgctgccca ccggcacgct acgcagagcc atggcattcc cggcgcgaat gggcgctccg
    12001 gtaccgctgc gaagctcgtc gccggcgctg acg
//
LOCUS       DBNHLM010000014        11256 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1438243, whole genome shotgun sequence.
ACCESSION   DBNHLM010000014 DBNHLM010000000
VERSION     DBNHLM010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11256)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11256)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11256
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1438243"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="AC16QF_00830"
     CDS             <1..612
                     /locus_tag="AC16QF_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6447123.1"
                     /translation="IATLDDLSDGRVSLGVGVAVTEDEFENLGVDFATRGRRADDALA
                     AIKALWTQEFPRHEGPFYQFSGMRFEPKPRQSPHPPILVGGGAPPALRRAARFADGWH
                     ALGQSPESLAESMKMLEESCRAVGRDVADLDISIRCVIEIVGEPWDRPVTQRRTLKGT
                     EREIAETIAAYAEAGVDEIVLDANLTDLEATRAVMHRGIRFQD"
     gene            694..1656
                     /locus_tag="AC16QF_00835"
     CDS             694..1656
                     /locus_tag="AC16QF_00835"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR6447124.1"
                     /translation="MDLPEEQMIRMLETMVRIREFDERAALLMEQARIHGSVHLYCGE
                     EAVGVGVCEALNPDDYITSTHRGHGHLIAKGGDPRLMYAELFAKSTGYNRGKGGSMHI
                     AALELGMIGANGIVGAGAPIALGVAFASAYQDNGRVTACFFGDGATNEGAFHEALNMA
                     AILKAPVVFVCENNLYGEWTPASQQTSVRDIADRSASYGIPGVIVDGMDIMAVHEAVV
                     EAVARARRGEGPTLLECKTYRFYDHVGRDFGILKRDPDEVAAWRARDPITLWRAELIA
                     QGVLDEAGADAITERAQAEMEDAIAYAEGSPDPEPEDLMRDIYS"
     gene            1674..2642
                     /locus_tag="AC16QF_00840"
     CDS             1674..2642
                     /locus_tag="AC16QF_00840"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017904614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR6447125.1"
                     /translation="MTVKTTTAAVAEAIDQEMARDDRVFVAGEDVRVGGPFGTDAGLH
                     AKYGDRRVVNTAISELALAGLGVGAAAVGLRPIIEIMIMDFMCVAMDQIVNQMAKMKY
                     MLGGKPTLPIVVTTHAGIDSSSAAQHSGSLEAWFCHVPGLKVVMPSNAYDAKGLLISA
                     IRDDSPVIFIHNKRCLQTSADVPDEAYTVPLGVADVKREGSDVTIVAIGRMVDEALEA
                     ADELQGDGVSAEVIDPRTLSPLDMETILDSVRKTTRAVVAHEAVRFCGIGAEIAATIS
                     DEAFDYLDAPVKRVAAPFTPIPFAPVLENLYLPTRESILAAVREIV"
     gene            2659..3111
                     /locus_tag="AC16QF_00845"
     CDS             2659..3111
                     /locus_tag="AC16QF_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6447126.1"
                     /translation="MTLDELLEIEAIKQMRYLYSHYYDGNMLDELISLFTEDAVCEYG
                     EDFGGDWVGKAQIRERYHDFLYGSGRAVHSQMHAVTNPWIRLKSDTQALGRWYQLNLN
                     VAEGAENPLTLFGIYDDVYRKVDGEWKIHRTRIDFLWPRRHFYGIRDA"
     gene            3104..4732
                     /locus_tag="AC16QF_00850"
     CDS             3104..4732
                     /locus_tag="AC16QF_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007944983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6447127.1"
                     /translation="MPEGPERFDAVVVGAGFAGLYMLLRLRELGMRVVVFEAGDGVGG
                     TWYWNRYPGARCDAESMGYSYSFSPELEQEWEWTERYATQPEILRYADHVAERFDLKR
                     DIRFETRVVAANYREAGNVWAVATDRGDEVEARFCIMATGCLSVPQRPDIPGLDDFQG
                     PTYVTGEWPHEGVDFTGLRVGLVGTGSSAIQAVPVIAAQAEHLTVFQRTANFSVPAKN
                     RPLDPDWVREFKERYRDYREDSRHGLVSDFGALVLPDAERKRAGGSFRSVPAAERDAR
                     YEKLWEVGGAYFMAAFEDTLLDPEANESAAEFVREKIREIVEDPETAERLCPTSHPIG
                     TKRICVDTGYYETYNRPNVTLVDISGTPIDTVTPKGLRVRNVDYEFDVLVLATGFDAM
                     TGALLGMDIRGVGGERLADHWRAGPRSYLGLCVTGFPNLVTITGPGSPSVLSNMMVSI
                     EQHVDWVADCLGHMADRGLTRLEALQQAEDGWVDHVNEVANATLFPKGGSWYLGANIP
                     GKPRVFMPYAGGVGPYRDICDGVAARGYEGFSIT"
     gene            4822..5982
                     /locus_tag="AC16QF_00855"
     CDS             4822..5982
                     /locus_tag="AC16QF_00855"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6447128.1"
                     /translation="MAESKRDTSADYGPHEEAMGAYLREGEARALALGNRGPIRFGAD
                     GRLAADILDAYSRLGFYVFTGVLSAEELDDLRSDVEDMWSRLPVSRGAAVDALGRPAL
                     GADLRAPTVQWARPLSDPLGGTDKANGRHPAKMFEPQPPADAPPFVCQLVLGSLQFSD
                     ACLRVYGHPQLLAVAAAINGDDFAPFNEAVWRKEPGLGPSVAWHQDGTTHWDSPDLDE
                     GTHGFNFMAQIYGCTAANAVWVVPGSHARGKVDIAALVARAGSDRLPDAVPLICDPGD
                     VAICNRQAVHGSFANTGGDLRVTVNFGFHRRQSLLGVEGGGIHNRREIYDERRIRERS
                     RVIGYAIDARRQRFPDEAPYVYRPFAGEEDEYRWSDAARARLKDYNLLDLGI"
     gene            complement(5988..6368)
                     /locus_tag="AC16QF_00860"
     CDS             complement(5988..6368)
                     /locus_tag="AC16QF_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6447129.1"
                     /translation="MAIRELVDSYNDAVMRFDGDAWAANWRDDATWSLPGAGPIKGKE
                     NFFPMWQQAMSAFSFVGFFASAGPIVVDGDTAHATWYQQEFLHQKDGVKRNVTGRYED
                     DYVKVDGRWYFQQRIYEVMNAEES"
     gene            complement(6496..7263)
                     /locus_tag="AC16QF_00865"
     CDS             complement(6496..7263)
                     /locus_tag="AC16QF_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6447130.1"
                     /translation="MIDVYYWPTPNGWKVTIMLEECGLPYRILPVDIGGGDQFTPDFL
                     RVSPNNRMPAIVDHEPLGGGEPLSIFESGAILEYLADKSGKFLSREPAGRYRTLQWVH
                     WQMANLGPMMGNANHFKNYGKNIAEDPQQLEYGTRRFVGEVDRLSGVMDAQLRVNRYL
                     AGSEYTIADIITWPWAMLLGRLIDEAVWETFPNLKRWVDEVGERPAVEKGRKVERDLG
                     RQERTEEQEKARRELLFNQTNEKVRVAREAAAKAAAG"
     gene            7425..8633
                     /locus_tag="AC16QF_00870"
     CDS             7425..8633
                     /locus_tag="AC16QF_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447131.1"
                     /translation="MLNVETLRVTLVAAMVLLAAQPAHADGTYGADFRAHLLPEAGTA
                     HASITITQEAGELKRLDLAAPAATYSAFSGPGSIRREEDRLVWTVPEDGGTLSYDVLI
                     DHRRNGAYDARMTPDWAIARLDDLFPRARTRSESGAQSRSSVVLAGPAGWRFETRYGP
                     SHEQVAVVDEGRRFDRPTGWLVAGRLGIRRDTIAGRTVSVAAPVGQGFRRVDVLAFLR
                     WTLPDLVAVFPGMPDRLLVVGAPEGMWRGGLSGPESLYLHVGRPLISENGTSTLLHEL
                     VHIATTAPKSAGDDWIMEGLAEYYSLETLRRSGGISEERFASALGSLAAWAQRDGGRL
                     ADPSTGAHTARAALTFHELAAELEDAGSSLDAVVGELVAGGSLDRGSLLALLEPKLTE
                     PSGILAELPR"
     gene            complement(8661..10208)
                     /locus_tag="AC16QF_00875"
     CDS             complement(8661..10208)
                     /locus_tag="AC16QF_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6447132.1"
                     /translation="MYPGKHVTEHAERPAIIMASTGEVITYKELEARTNQLAHLLRDQ
                     GLKRLDHYAIFMENNSRYVEACGAGERSGLYYTCINSYLTPEEVAYIVNNSESRVLIT
                     SREKAEVAKAALADCPRVALCLVVDGDDDARVINYESAIERFPSTPIPDESLGTAMLY
                     SSGTTGRPKGIIRPLPEAPPEQDLPLFQFINGIWRFREGMIYLSPAPLYHSAPQGSVN
                     LTIRRGGTAVIMERFDPEQFLELVERYRITHSQLVPTMFSRMLKLPEEIRARADVSSL
                     EIAIHAAAPCPVQVKEAMIDWWGPIIYEYYGATEGLGFTGCTTEEWLAHKGTVGRVLL
                     GELHILDEDGNPSPKGEPGEVWFKTASAFEYFNDPEKTAQATSADGSMTTVGDVGYVD
                     DDGYLYLTDRATFMIISGGVNVYPQECENLLITHPKVADAAVFGVPNVDLGEEVKAVV
                     QVMPDVEPGEALEAELIAFCHEHLARQKCPRSVDFEEQLPRLPTGKLYKRHLRDRYWG
                     DKKSRIV"
     gene            complement(10228..11001)
                     /locus_tag="AC16QF_00880"
     CDS             complement(10228..11001)
                     /locus_tag="AC16QF_00880"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6447133.1"
                     /translation="MSRTVQDLFDMSGHVAVVTGGGTGIGAGIARAFHESGAAVILAA
                     RRTENIERIAKEIRDDGGSAIAVTTDVTDDDAVDALARAAVEEFGRLTDWVNNAGGSP
                     MRMPLSELPREEWDRTIALNLTSIYVGCVTAARHMEHGSIINITSGAGTGPVPGSAHY
                     GASKAGTNSLTWTLAAEFAPRIRVNAVAPGAIPTEVMLKAIGRTEDELDKVLEEWNIP
                     LGRLGTPQDIGAACVYLASDAASWVSGEIIRVGGGARPR"
BASE COUNT         1811 a         3627 c         3829 g         1989 t
ORIGIN      
        1 atcgccacgc tggacgacct ctccgacggc agggtcagcc tgggggtcgg ggtggcagtc
       61 accgaggacg agttcgagaa cctcggcgtc gacttcgcta cccggggccg gcgcgcggac
      121 gacgcgctgg ccgccatcaa ggcgctctgg acccaggagt ttccccgcca cgaaggcccc
      181 ttctatcagt tctccgggat gcgtttcgag ccgaaaccac ggcaatcgcc gcacccgccc
      241 atcctggtgg gcggcggcgc gccgcccgca ctccggcgcg ccgcgcggtt cgcagacggc
      301 tggcacgcgc tcgggcagag cccggagtcc ctggcggagt cgatgaaaat gctggaggaa
      361 tcgtgccgcg cggtgggtcg ggacgtggcg gatctcgaca tcagcattcg ctgcgtcatc
      421 gagatcgtag gggaaccctg ggatcgaccg gtgactcagc gccggacgct gaaaggcacg
      481 gagagagaga tcgcggagac catcgccgcc tacgccgagg cgggcgtgga cgagatcgtt
      541 ctcgatgcga acctgaccga cctggaggct acccgcgccg tcatgcaccg gggaatccgg
      601 ttccaggact gagccggccg ctccttgcgg cgctgtttga cctggttggc gggcgtcgtt
      661 agccttcgat gtcttattct caggagcgct gccatggatc ttcccgaaga gcagatgatt
      721 cgcatgctcg agaccatggt ccggatccgc gagttcgacg agcgggctgc gctgctcatg
      781 gagcaggccc gcatccacgg ctcggtgcat ctgtattgcg gggaggaggc cgttggcgtc
      841 ggcgtctgcg aagcgctgaa cccggacgac tacatcacct cgacacaccg gggccacggg
      901 cacctcatcg ccaaaggcgg cgatccccgg ctgatgtatg cagagttgtt cgccaagtcc
      961 accggctaca accggggtaa gggcggctcc atgcacatag ccgcgctgga actgggcatg
     1021 atcggtgcca acggcatcgt cggcgcgggg gcgcccatcg cgctaggcgt cgccttcgcc
     1081 agcgcttatc aggacaacgg ccgggtcacg gcctgtttct tcggcgacgg cgcgaccaac
     1141 gagggcgctt ttcacgaagc gctcaacatg gctgccattc tcaaggcgcc ggtggtcttc
     1201 gtttgcgaga acaacctcta cggcgagtgg acgccggctt cccagcagac gtcggtgcgg
     1261 gacatcgccg atcgttcggc ctcatacggc attccggggg tcatcgtcga cggcatggac
     1321 atcatggccg ttcacgaggc ggtggtggaa gccgtggcaa gagcccgcag gggcgagggg
     1381 ccaacgctcc tggaatgcaa gacgtaccgc ttctacgacc acgtcgggcg cgacttcggc
     1441 attctcaagc gggatcccga cgaggtggcc gcatggcgcg cccgggaccc catcacgctc
     1501 tggcgggccg agctgatcgc tcagggggtg ctcgacgaag ccggcgccga cgccatcacg
     1561 gagcgcgccc aggcggagat ggaggacgcc atcgcgtatg cggagggcag cccggatccc
     1621 gagcccgagg acctgatgcg cgacatctac agctgaatat cgaagggggc agggtgacag
     1681 taaagacgac gacggccgcg gtggcagagg ccatcgacca ggaaatggcg cgggacgaca
     1741 gggtgttcgt cgccggagag gacgtccgcg tgggcgggcc gttcggcacg gacgctggtc
     1801 ttcacgccaa gtacggcgat cggcgcgtgg tgaacacggc gatatcggag ctggccctcg
     1861 cgggcctcgg ggtcggggcc gcggcggtgg gtctgcgacc catcatcgag atcatgatca
     1921 tggacttcat gtgcgtggcc atggaccaga tcgtgaacca gatggccaag atgaagtaca
     1981 tgctcggcgg aaagcccacg ctgcccatcg tggtgacgac ccacgcgggt atcgacagca
     2041 gcagcgcggc ccagcactcg ggcagcctcg aggcgtggtt ctgccacgtg cccggcctga
     2101 aggtggtcat gccttccaac gcctatgacg ccaagggcct gctgatctcc gccatccggg
     2161 acgacagccc ggtgatcttc attcacaaca agcgctgcct gcagacctcg gccgacgtgc
     2221 ccgacgaggc ctacacggtt cccctcggcg tggccgacgt caagcgggag ggcagcgacg
     2281 tcaccatcgt ggccatcggc cgcatggtcg acgaggctct ggaagcggcc gacgagctgc
     2341 agggcgatgg tgtcagcgcc gaggtgatcg acccgcggac cctgtcgccg ctggacatgg
     2401 agacgattct cgactccgtt cgcaagacga cgcgggcggt ggtggcccac gaggcggtca
     2461 ggttctgcgg catcggtgcc gagatcgcag cgaccatctc cgacgaggcg ttcgactacc
     2521 tcgacgcgcc ggttaagcgg gtggccgcgc cgttcacgcc gattcccttc gcgccggtcc
     2581 tggaaaacct ctatctgccg acccgggaat ccatcctggc ggccgttcgg gaaatagtct
     2641 gaacaggagg agccggccgt gacccttgac gagttgctcg agatcgaagc catcaagcag
     2701 atgcgttacc tgtactcgca ctactacgac ggcaacatgc tcgatgagct gatcagcctc
     2761 ttcaccgagg acgccgtatg cgagtacggc gaggatttcg gcggcgactg ggtgggcaaa
     2821 gcgcagattc gcgagcgtta ccacgacttc ctctatggca gcggacgcgc ggtgcattcc
     2881 cagatgcacg cggtgaccaa tccgtggatc cgtttgaaga gcgataccca agcgctcggc
     2941 cgctggtacc agctgaacct gaacgtcgcc gaaggcgcgg agaacccgct gaccctcttc
     3001 ggcatctacg acgacgtgta tcgcaaggtg gacggggagt ggaagatcca ccggacccgc
     3061 atcgacttcc tctggccccg gcgccacttc tacgggatcc gggatgcctg aaggcccgga
     3121 acgcttcgac gccgtcgtcg tcggcgccgg ctttgccggg ctttacatgc tgctgcggct
     3181 ccgtgagctg ggcatgcggg tggtggtttt cgaggcgggt gacggcgtcg gcggcacctg
     3241 gtactggaac cggtatccgg gcgcccgctg cgacgcggag agcatgggct actcgtactc
     3301 gttctcgccc gagctcgagc aggagtggga gtggaccgag cgctacgcca cccagcccga
     3361 gattcttcgc tacgccgacc atgtcgccga gcgcttcgac ctcaagcggg acattcgctt
     3421 cgagacgcgg gtggtggcgg ctaactatcg ggaagccggc aacgtctggg ccgtggccac
     3481 ggaccgcgga gacgaggtgg aagcccgctt ctgcatcatg gcgaccgggt gcctgtccgt
     3541 tccccagcgt cccgacatcc cgggcctgga tgactttcag ggcccgacgt acgtgaccgg
     3601 agaatggcct cacgaggggg tggatttcac cggcctgcgg gtcggccttg tgggcacggg
     3661 ctcatcggcc atccaggccg tgcccgtcat cgccgcccag gccgagcacc tgacggtatt
     3721 tcagcgcacg gcgaatttca gcgttcccgc caagaaccgt ccgctggatc ccgactgggt
     3781 gcgcgagttc aaggagcgct accgggacta tcgtgaggat tcgcgacacg ggctggtgtc
     3841 cgacttcggc gccctcgtgc tgcccgacgc cgagcgcaag cgggccggcg gatcgtttcg
     3901 ttccgtgccg gcggccgagc gcgatgcccg ctacgaaaag ctctgggagg ttggcggcgc
     3961 gtatttcatg gcggccttcg aagacaccct gctggatccc gaggccaacg agagcgcggc
     4021 ggagttcgtg cgtgagaaga tccgtgagat cgtcgaggat ccggaaaccg cagagcgcct
     4081 gtgccccacg tcgcatccca tcggaaccaa acggatctgc gtggacaccg gctattacga
     4141 gacctacaac cgccccaacg tgacgctggt ggacatctcc ggtaccccca tcgataccgt
     4201 cacgccgaaa gggttgcggg tgcgcaacgt cgactacgag ttcgacgttc tggtgctcgc
     4261 cacgggcttc gacgccatga ccggcgcgct gctcggcatg gacatccggg gtgtcggcgg
     4321 ggagcggctg gccgatcact ggcgtgcggg cccgcggagc tacctgggcc tgtgcgtcac
     4381 cgggtttccc aacctggtga ccatcaccgg cccgggcagc ccctctgttc tgagcaacat
     4441 gatggtttcc atcgagcagc acgtggactg ggtggccgac tgcctgggcc acatggccga
     4501 tcgcgggctc acccgcctgg aggctctgca gcaggctgag gacggttggg tggatcacgt
     4561 caacgaggtc gccaacgcca cgctgttccc caagggaggg tcctggtatc tcggcgcaaa
     4621 cattcccggc aagccgcgcg tgttcatgcc ctatgcgggc ggcgtgggcc cgtatcgtga
     4681 tatctgcgat ggcgtggccg cgcgcggcta cgagggcttt tcgatcacct agcggcgccg
     4741 cagcgcgggt cgacggcaag gcttgcccga ggcgatactt cgggggcgcg caccgcgatg
     4801 cggatcgatc gggagatagg tatggctgag tcgaagcggg acaccagcgc cgactacggg
     4861 ccccacgagg aggccatggg cgcctatctt cgcgaaggcg aggcgcgcgc ccttgccctg
     4921 ggtaaccggg gccccatccg cttcggcgcc gatggtcggc tcgcggcgga catcctcgac
     4981 gcctactcgc gcctgggctt ctacgtgttc accggggtgt tgtcggccga ggagctggac
     5041 gatctccgtt cggacgtcga ggacatgtgg tcgcgactgc cggtctcccg gggtgctgcg
     5101 gtggacgcgc tgggtcgtcc cgcgctgggc gccgaccttc gcgcacccac ggtgcagtgg
     5161 gcgcggcccc tcagcgatcc cctgggcggc acggacaaag ccaacggtcg gcacccggcc
     5221 aagatgttcg agccgcagcc gccggccgat gcgccgcctt tcgtctgcca gctggtgctc
     5281 gggtccctgc agttctccga tgcgtgcctc agggtttacg gccatcctca gctgctcgcg
     5341 gtggcggcag ccatcaacgg ggacgatttc gcgccgttca acgaagccgt ctggcggaag
     5401 gagccgggtc tgggcccgtc ggtcgcctgg caccaggacg gtaccaccca ctgggacagt
     5461 cccgatctcg acgagggaac ccacggattc aatttcatgg cgcagattta cgggtgcacg
     5521 gcggcgaacg ccgtctgggt cgtgccggga tcacacgctc gcggaaaagt tgacatcgcc
     5581 gcgctcgtcg cccgcgcagg ctcggatcgc cttcccgacg cggtgcccct catctgcgat
     5641 cccggagatg tggccatctg caaccgtcag gcggtacacg gatccttcgc caataccggg
     5701 ggcgatctga gggtcaccgt caacttcgga tttcaccgac gccaatcgct gcttggcgtg
     5761 gagggcggcg gcatccacaa ccgccgggag atatacgatg aacggcgtat ccgcgagcgg
     5821 tcgcgtgtga tcggttacgc catcgatgcg cgcaggcagc gctttcccga cgaggcgccc
     5881 tacgtctatc gaccgttcgc aggcgaggag gatgagtacc gctggagcga cgcggcccgc
     5941 gcgcggctca aggactacaa cctcctcgac ctggggatct aaggcgctta cgactcctcg
     6001 gcgttcatga cctcgtagat gcgctgctgg aagtaccagc gcccgtccac cttgacgtag
     6061 tcgtcctcgt aacgtcccgt gacgttgcgt ttcacgccgt ccttctggtg gagaaactcc
     6121 tgctggtacc aggtggcgtg agcggtgtcg ccatccacca cgataggccc ggccgaggcg
     6181 aagaagccga cgaaggagaa cgcgctcatg gcctgctgcc acatgggaaa gaagttctct
     6241 tttcccttga tcggtccggc cccgggcaga gaccaggtgg cgtcatcgcg ccagttggcc
     6301 gcccacgcat ctccatcgaa acgcatgacg gcatcgttgt acgagtcgac tagctcgcga
     6361 attgccatgc ggtcttccag tggtccactg ctgatcattt gcttccctcc cttcttggtc
     6421 ttgaatgcgg tctcgaagaa tcacacttaa tcacgacgcg ctgcgcattg cgagcgcgtg
     6481 gcgaggtccg gcccgtcagc cggcggccgc tttcgccgcg gcctctcgtg ccacacgcac
     6541 tttttcgttg gtctggttga acaggagctc ccgccgggct ttctcctgtt cctcggtccg
     6601 ctcctggcgg ccgagatcgc gttccacttt gcggcccttt tccacggcgg ggcgttcgcc
     6661 cacctcgtcc acccagcgct tgaggttcgg aaaggtctcc cagacggcct cgtcgatgag
     6721 gcggccgagc agcatggccc acggccaggt gatgatgtcc gcgatggtgt actcgctgcc
     6781 ggcgagatac cggttgacgc ggagctgagc gtccatcacg ccggacagac ggtccacttc
     6841 gcccacgaaa cgacgggtgc cgtattccag ctgctgcggg tcctcggcga tgttcttgcc
     6901 gtagttcttg aagtgattgg cgttgcccat catcgggccc agattggcca tctgccagtg
     6961 aacccactgc agggtgcggt atcggccggc cggctcccgg gacagaaact tccccgactt
     7021 gtcggcgaga tactcgagta tggcgccgga ttcgaagatc gacagcggct ccccgccgcc
     7081 gagaggctcg tgatcgacga tggccggcat gcggttgttg gggctcacgc gcagaaagtc
     7141 cggcgtgaac tggtcgccgc cgccgatgtc gaccggcagt atccggtagg gcaggccgca
     7201 ctcctccagc atgatggtga ccttccagcc gttcggggta ggccagtaat acacgtcgat
     7261 catcgcttcg ctcctctcag cgccagtcgc gtccgcgcgt acactatggg agattcaatt
     7321 tctcgattca aacgtcggcg cgcttccaat cccgacggcg ctctggtatc tgcgctgtgt
     7381 catccagccg ttatcgcgaa ggtgccgtgt ggagtcgtca aacgatgctg aacgttgaga
     7441 ccctgagggt caccctggtg gcggcgatgg tgctgctcgc cgctcaaccc gctcacgccg
     7501 acgggactta cggcgctgat ttcagggcgc acctgcttcc cgaagcggga acggcgcatg
     7561 cctcgatcac cattactcag gaggcgggcg agctcaagcg cctggacctg gcggccccgg
     7621 cggcgaccta ttcggccttt tcaggtcccg ggagcatccg gcgcgaggaa gatcgtctgg
     7681 tctggaccgt tcctgaggac ggcggcaccc tgagctacga cgtgttgatc gaccatcgcc
     7741 gcaacggggc ctacgatgcg cggatgacgc cggattgggc catcgcgcgg ctggatgatc
     7801 tctttccgcg cgcccggacg cgcagcgagt cgggggcgca atcccggtcg agcgtcgttc
     7861 ttgccggacc ggcgggctgg cggttcgaga cccgctacgg gccgagccac gagcaggtgg
     7921 ccgtggtgga cgaggggcgc cggttcgacc ggcccaccgg ctggctggtg gccggcaggc
     7981 tcggcatccg cagggatacc atcgccggcc ggacggtttc agtggcggcg ccggtggggc
     8041 agggctttcg ccgggtggac gtgctcgcct tcctgcgctg gacgctgccc gatctcgtcg
     8101 ccgtgtttcc cggaatgccc gaccggctgc tcgtcgtagg tgccccggag ggcatgtggc
     8161 gagggggcct ttccggcccg gaatcgctgt acctccacgt cgggcgcccg ctcatcagcg
     8221 agaacggcac cagcacgctg cttcatgagc tcgtgcacat cgccacgacg gcgccgaaat
     8281 cggctggcga cgactggatc atggaagggc ttgccgagta ctacagcctg gagacccttc
     8341 ggcgcagcgg gggtatcagc gaggagcgct tcgcgtcggc cctggggtca ctggcggcct
     8401 gggcgcaacg tgacggcggg cgcctggcgg acccatccac gggtgcgcac acggcccgcg
     8461 cggcattgac gtttcatgag ctcgctgcgg agctcgagga tgcgggcagc agcctcgacg
     8521 ccgttgtggg agagctggtc gcaggcggca gcctggatcg cggcagcctg ctggcgttgc
     8581 tggagccgaa gctcaccgag ccatcaggaa ttcttgccga actcccccgt tgaggcgggc
     8641 gcccgcctgc ctgtttcagc tcagacgatg cggctttttt tgtcgcccca gtaccggtcg
     8701 cgcaggtgac gcttataaag cttgcccgta ggcaggcggg gcagctgttc ctcgaagtcg
     8761 acggagcggg ggcacttctg gcgggcgagg tgctcgtggc agaaggcgat gagctccgct
     8821 tccagcgctt cccccggttc cacgtccggc atgacctgga ccacggcttt cacctcttcg
     8881 ccgagatcca cgttgggcac gccgaacacc gcggcgtcgg caaccttcgg atgggtgatc
     8941 agcaggttct cgcattcctg gggatagacg ttcaccccgc cggagatgat catgaacgtc
     9001 gcccggtcgg tgaggtacag gtagccgtcg tcatccacgt agccgacgtc gcccacggtg
     9061 gtcatgctgc cgtctgccga ggtggcctgc gccgtcttct cgggatcgtt gaagtactcg
     9121 aaggccgacg cggtcttgaa ccagacctcg cctggctcgc ctttcgggga agggttgcca
     9181 tcctcgtcga ggatgtgcag ctctcccagc agcacccggc ccacggtgcc cttgtgcgca
     9241 agccactcct cggtggtgca gcccgtgaag cccaggcctt ccgttgcgcc gtagtactcg
     9301 tagatgatgg ggccccacca gtcgatcatc gcttccttga cctgcaccgg gcatggcgct
     9361 gccgcgtgga tggcgatctc cagggacgag acatcggccc gggcgcggat ctcctcgggc
     9421 agcttcagca tgcggctgaa catcgtcggc acgagctggc tgtgggttat gcggtaacgt
     9481 tcgaccagtt ccaggaactg ctcggggtcg aagcgctcca tgatcacggc ggtgccgccc
     9541 cgccggatgg tgaggttgac ggatccctgg ggagcggagt ggtaaagagg cgccggcgag
     9601 aggtagatca tgccttcgcg aaagcgccag atgccgttga tgaactggaa gagcggcaga
     9661 tcctgctccg gcggtgcttc cggcaggggc cggatgatgc ccttgggacg cccggtggtg
     9721 ccggaggagt agagcatggc cgtgccgagg ctctcgtcgg gaatcggcgt cgaaggaaat
     9781 ctttcgattg cgctttcgta gttgatcacc ctggcgtcgt cgtcgccgtc gacgaccagg
     9841 cacagcgcta cccgggggca gtccgcgaga gccgccttcg ccacctccgc cttctccctg
     9901 gaagtgatga gaacgcggga ttcgctgttg ttgacgatgt aggccacttc ttccggcgtg
     9961 aggtacgagt tgatgcacgt gtagtagagg cccgatcgct cgcctgcgcc gcaggcttcc
    10021 acgtagcggc tgttgttctc catgaagatg gcgtagtggt cgagcctttt gaggccttgg
    10081 tcgcgcagga gatgggccag ctggttggta cgtgcttcga gctccttgta cgtgatgacc
    10141 tctccggtcg aggccatgat gatggcgggc ctttcggcgt gctccgtgac gtgcttgccc
    10201 gggtacatga cgaacctccc tcgacggcta gcgcgggcgg gcgccgccgc ccacgcgaat
    10261 gatctcgccg ctcacccagc tggccgcgtc cgaggcaagg tagacgcagg cggcgccgat
    10321 gtcctggggg gtacccagac gccccagcgg aatgttccac tcctccagca ccttgtcgag
    10381 ctcgtcctcg gtcctgccga tggccttgag catgacctcg gtggggatgg cccccggcgc
    10441 taccgcgttg acccggatgc ggggcgcgaa ctcggcggcc agcgtccagg tgagcgagtt
    10501 ggttccggcc ttggaggccc cgtagtgggc gctgcccggc accgggccgg ttccggcccc
    10561 agaggtgatg ttgatgatcg acccatgctc catgtgccgc gctgccgtga cgcaacccac
    10621 gtagatcgag gtgagattca aggcgatggt cctgtcccac tcctcccgcg gcagctcgct
    10681 gagcggcatg cgcatgggcg agcccccggc gttgttgacc cagtcggtga gccggccgaa
    10741 ctcctccacg gccgcgcggg ccagcgcgtc gacggcatcg tcgtcggtga cgtcggtggt
    10801 gacggcgatg gcgctgccgc cgtcgtcacg aatctccttc gcgatgcgct cgatgttctc
    10861 ggtccggcgc gcggcgagga tcacggcagc gccggattcg tggaaggccc gggcgatgcc
    10921 ggcaccaatg ccggtgccgc ccccggtgac gacggccacg tgcccgctca tgtcgaacag
    10981 gtcctgaacg gttctgctca tgcccacgct ctcctccccc ttggtgcctg ggattatcac
    11041 acaccggccg cttcaggcga tcgttgagtg cccgtgccgg tcttgctaac gttcgggaat
    11101 tcttgagctg gagcgatcag tgagcgagaa caacgccgtt gaggaaacca aggccgtgct
    11161 gggacgccgg ggccacgtca ccacgtcctg ggacgagctg gaaacgctgc agctttccac
    11221 ggggatcgaa accacgatgt tcaggatcgg gatgcc
//
LOCUS       DBNHLM010000015        11087 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_600268, whole genome shotgun sequence.
ACCESSION   DBNHLM010000015 DBNHLM010000000
VERSION     DBNHLM010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11087)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11087
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_600268"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2415)
                     /gene="pepN"
                     /locus_tag="AC16QF_00885"
     CDS             complement(<1..2415)
                     /gene="pepN"
                     /locus_tag="AC16QF_00885"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HIR6447134.1"
                     /translation="MSSNVPQPVFLRDYEPPSYRTLDTRLSFDVRDGYTRVESQLSVE
                     RRAGGTDTVLRLDGQNLELLSVALDGRLLGGNEFVVDAESLTLFDLPERCEITIVTRI
                     EPEKNTALEGLYKSGSMYCTQCEAEGFRRITYYQDRPDVLARFTTTLEADASLYPVLL
                     ANGNAISDVPAGEGRRRVTWEDPFPKPSYLFALVAGDLSVMEDEFVTASGRRVALRIF
                     SEPQNIGQCGYAMEALKRAMRWDEETFGREYDLDILMIVAVEDFNMGAMENKGLNIFN
                     TKCVLASPDTATDAAHLRVESVVAHEYFHNWSGNRVTCRDWFQLSLKEGFTVFRDEEF
                     SSDMNFRAVKRIEDVNFLRSSQFPEDAGPMAHPVRPDSYIEISNFYTTTVYEKGSEVV
                     RMLRTLLGEERFRRGSDLFFDRYDGCAVTTDDFLGAMADVTALDLKQFRRWYEQAGTP
                     RLQVEEGWHDGELNLRIRQSCPPTPGQGSKKPFHLPVLIGLLDGAGRDLVDGGLDCSS
                     DAQVDVREGDRSLLVHVRDEITEIRIPGLPGRPAVSFLRGFSAPVRVEYPRDPETLAF
                     LALHDSDGFARWDALQTLVVREIDAMRSGGEPAAELVALLGNFIDPALAGGASAEQRF
                     LLSELLHVADEGYLFQALDDFEVEAVCDARDRLVVSVAANHAERWRALYEANAASGAY
                     APEPDGMSRRALRNTALGYLARVETGSALESLLEDHYRSADNLTDRSAALRAVADVDG
                     LSDSFRSEMLDEFHRRWRAHALIVDLWFSVQASSPRSSAGTIRELESHPDFDGLNPNK
                     LRALHGA"
     gene            complement(2457..3335)
                     /locus_tag="AC16QF_00890"
     CDS             complement(2457..3335)
                     /locus_tag="AC16QF_00890"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR6447135.1"
                     /translation="MLLPLWLCFMRVIETDLDEDLSVLSRLLWERRVRHRIYEERGRQ
                     IVELADGAQAALVRDVYEALASGSLVVEAQPLPQRRPGLLAQIRRYPGLAVLVALAVA
                     LFPFTMPLAEGRVTYVASWLTIVDLIRLGSAPGTLVELVAEAAVWRWFAPMLIHFNAV
                     HLLFNCAVVIEMGRRIESRLGALWFWAIVLVVSLVANLGQYAMNANPLFGGLSGVAFG
                     LLGFVLAAGRLAPAVSQWQVPTAVAGGLLFFLVLFSTGVTEYFGLHVANAAHWFGLAA
                     GAVLGIVTGRLVGSGR"
     gene            3334..4368
                     /locus_tag="AC16QF_00895"
     CDS             3334..4368
                     /locus_tag="AC16QF_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR6447136.1"
                     /translation="MQAYQIGATDEMHAVDEEVLNQVTEWLLADRPCWLATVVATWGS
                     SPRPVGSLLACNAEGQIVGSLSGGCVEDDLLEKLTSGELAAEHAQTFEYGVTAEESEK
                     FGLPCGGSLLIVIEPVAANAAYKAHMSHIRDQLAQRRCVKRTVDLKNRSFRTLQVPQH
                     EPLRWEADSQILEHTYGPRYQLFVIGAGMVSKYLAEMAQMLDYQVTVCDPREDLLNDF
                     GIPGVKLVADMPDDAIKAHASDPASAIVALTHDPRIDDMGMMQALTTDAFYVGAMGSA
                     RTSASRRERLAALELSEEQIGRLHAPIGLPIGSKTPPEIAVAILAEITAVRKARAESA
                     CAHLAQLSAG"
     gene            4375..4962
                     /locus_tag="AC16QF_00900"
     CDS             4375..4962
                     /locus_tag="AC16QF_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR6447137.1"
                     /translation="MRGGVVLLAAGSASRFGSDKRTHSLPDGRSLLGATIAKYARAFA
                     EIIVVLGPADRALAEELRRQFDPQTVTIVHAQAARLGMGHSLAAGIRAARGWDFAFVA
                     LGDMPFVQPQTLAALGRAMSEAGHDAIVQPFHEGHKGHPVGFGRRHFSALEALTGDEG
                     AREVVKTSASDLVPLEVDDPGVLQDVDTPADLAGD"
     gene            complement(4976..5863)
                     /locus_tag="AC16QF_00905"
     CDS             complement(4976..5863)
                     /locus_tag="AC16QF_00905"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011532967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HIR6447138.1"
                     /translation="MTGSFETVGLIGRSGKPRIRDSLRAVIDCMEANGRRVLLESDTA
                     RRMGKKLRHEQVSRQALGENCDLIIVVGGDGSLLGVGRDLAHARVPVLGVNRGGLGFL
                     ADVSPDQIAEKLAEVLKGDYVIEDHFLLNARIVRAGETLGHSPALNDVVVHMGGMARM
                     MEFSLWVDGEFVYDQRSDGLIVASPTGSTAYALSAGGPIMHPNLDAIVVVPMFPHTLT
                     SRPLVVTGESEIKILAAEDAEVTCDSQVDFALRSGDEVCISKFDHPLRLVYTLGHSFY
                     ESCRSKLDWATRLGGRNND"
     gene            5962..6813
                     /gene="cysE"
                     /locus_tag="AC16QF_00910"
     CDS             5962..6813
                     /gene="cysE"
                     /locus_tag="AC16QF_00910"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - L-serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HIR6447139.1"
                     /translation="MTSALDQRYSQVLTEALSQSAHGAGEPDDLWTLMRGEAHSKAAE
                     EPILGSYFHATILNHESFGSALSFRLASKLDNPMLPTMLIRDVIQEAMADDPAILRSA
                     EIDILATYARDPACTDLSTPFLFFKGFHALQAHRIAHWLWLQGRRTLAQFFQNQISVT
                     LGVDFHPAARIGCGVMLDHATGIVVGETAVVEDDVSILHAVTLGGSGKVSGDRHPKIR
                     RGVLLAAGCKVIGNIEVGEGAKVGAGSVVLENVPPHVTVAGVPARIVGQTDEGTVPAL
                     DMDQRLV"
     gene            complement(7189..8505)
                     /locus_tag="AC16QF_00915"
     CDS             complement(7189..8505)
                     /locus_tag="AC16QF_00915"
                     /inference="COORDINATES: protein motif:HMM:NF039910.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HIR6447140.1"
                     /translation="MLTAAQNEVLTLTGPDTDMGRVFRAYWQPVLLSREIEAEGAPKR
                     VKILGEEFVAFRNGRGEVGVVEPNCPHRGAHLFFGRNELEGLRCVYHGWQFDVSGRCV
                     DVPTADAKVAESIKPRAALRALSVKESCGIVWVHFGGGGGDVPQFDFMGLPEANYFVS
                     KKLQECNWAQAAEGGIDTAHFSYLHANIEGGERAGLMPSTGANEPPELGRYRWMIEDS
                     RPRFTVLEHEAGLVMCAARKADDDQLYWRLTQFMMPNHSMAPNSFPGDNHVGNTWVPI
                     DDHSCWIYCYAYNPERPLSDRERHAFASGFGIFSEVDDEFVPVRNRGNDYLIDRNLQR
                     YGSFTGITGISEQDAAIADSQGLIADRTRELLGQTDLGVVRFRQLMFEAVDAVRQGRP
                     PRGAESPGAYRVRSGDAMSAKPANALEVVQERFGDLGGVNLSVVGD"
     gene            8661..8762
                     /locus_tag="AC16QF_00920"
                     /pseudo
     CDS             8661..8762
                     /locus_tag="AC16QF_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611773.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(8965..10962)
                     /locus_tag="AC16QF_00925"
     CDS             complement(8965..10962)
                     /locus_tag="AC16QF_00925"
                     /inference="COORDINATES: protein motif:HMM:NF015585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HIR6447141.1"
                     /translation="MDRIPLAGLLVALAIGPASAPAEVSMPSLFSDHAVLQRDMPVPV
                     WGTSAPGAKIEILWDDDENPVATTVADASGVWQASIGPLQGDLEYHSLTVLENEFPSV
                     TINNVRVGEVWLGSGQSNMNRPLSGDGEAGLGLADAPHLNLSFFNVASGTPSNALWEP
                     SNGATAESMSAVLFWFGRRLERALASDPELVGLPIGLIHSSVGATAIERWSTVAGSGG
                     LYQEQIQPLQPYAIRGVLWYQGEWDARGAQDSSKYYWQLPALIEEWRADWGQGNFPFY
                     VVQMPRMGINSIHVIRDAELQTALVGVDVEMTVNVDWHEVDVHPSEYRTFGERLANIA
                     LRNVHGQDVVAFGPVADPTLSEVVADVLYVGFRHVEQGLATSDSRAPAEWEIAGADGN
                     YKAAEAVLTGDGRVTLSHPEVPVPVFARYAYATAPANPNLQNSAGLPASPIRELEVAS
                     SPDLDPPTPANMDWEIPPHATGPTSIAMTAVPASDPSGVEYNFTCVIPGLGCIDSGWQ
                     DSRQHENSGLLPATEYDFQVTARDKSPARNETQPSGIASATTEHDPPLCEPAESFHVA
                     AISVFTVKADRGAKRAQAQVVIENDCGQPVENADVRAIFSGDYNEEQTTPTNSAGLAA
                     FQSTDSVKGGVSFMFCVIDVVVGPDAPSYSPDDNVATCATF"
BASE COUNT         2126 a         3622 c         3433 g         1906 t
ORIGIN      
        1 cgccccatgc aaagcgcgca gcttgttggg attcaggcca tcgaagtcag ggtgcgattc
       61 aagctccctg atggtgccgg cgctgctgcg cggactgctc gcctgaaccg aaaaccacag
      121 gtcgacgatg agcgcgtgtg cccgccagcg ccgatgaaac tcgtcgagca tttcactgcg
      181 aaaagaatcg ctgagaccat cgacgtcggc gacggcccgg agggcggcgc tgcggtccgt
      241 gaggttgtcg gccgaccggt aatgatcctc gagcaacgac tccagagcgg acccggtctc
      301 gacccgggca agatacccga gggccgtgtt gcgcagggcc cggcgggaca tgccgtcggg
      361 ctcgggcgcg taggcacccg aggccgcgtt ggcctcgtaa agcgcgcgcc accgctcggc
      421 gtgattggcc gccaccgaca ccacgagccg gtctcgggca tcgcaaacgg cttctacctc
      481 gaagtcgtcg agcgcctgga agagataacc ctcgtccgcg acgtgcagta actccgacag
      541 cagaaagcgc tgctcggcgc tggccccacc ggccagcgca ggatcgatga agttgcccag
      601 cagcgctacg agctcggcgg cgggttcgcc gccggagcgc atggcgtcga tctccctgac
      661 caccagcgtc tgcagcgcat cccagcgcgc aaacccatcg gagtcgtgaa gagccagaaa
      721 ggcaagcgtc tcggggtcac ggggatattc cactcgaacc ggagcggaga acccccgcag
      781 gaaactcacc gccggacgcc cgggcagacc ggggatgcga atctcggtga tctcatccct
      841 tacgtgaacg agcagcgacc ggtcgccctc gcgcacatcg acctgggcgt cgctcgagca
      901 gtccaggccc ccatcgacca ggtcgcggcc ggcaccatcg agcagcccga tcagaacggg
      961 aaggtggaag ggcttcttgc tgccctgccc gggcgtcggc gggcaggact gccggatgcg
     1021 caggttcagc tcgccatcgt gccagccctc ctccacctgc aatcgtggcg tcccggcctg
     1081 ctcgtaccag cgccggaact gtttcagatc gagggcggtc acgtccgcca tggcgcccag
     1141 aaagtcgtcg gtcgttaccg cacagccgtc gtagcgatcg aagaagaggt ccgaaccacg
     1201 gcggaaccgt tcctctccga ggagggtccg cagcattcgg accacctcgc tgcccttctc
     1261 gtagaccgtc gtggtgtaga agttggagat ctcgatgtaa gagtcgggcc gcaccgggtg
     1321 cgccatgggg ccggcgtcct cgggaaactg cgacgaacgc agaaaattca cgtcctcgat
     1381 gcgcttgacc gcccggaagt tcatgtcgga ggaaaactcc tcgtcccgga aaacggtgaa
     1441 tccttccttc aagctcagct gaaaccagtc gcgacaggtg acgcggttgc cggaccagtt
     1501 gtgaaaatat tcgtgagcca cgacgctctc gacacgcaga tgggcggcat ccgtggcggt
     1561 gtccggcgaa gccagaacgc atttggtgtt gaagatgttc aggcccttgt tctccatggc
     1621 gcccatgttg aaatcttcca ccgccacgat catgaggatg tcgagatcgt actccctgcc
     1681 gaacgtctcc tcgtcccagc gcatggcgcg cttgagcgct tccatggcgt aaccgcactg
     1741 gccgatgttc tgtggctccg agaagatccg aagcgccacg cgacgtccgg aagcggtcac
     1801 gaactcgtct tccatgaccg agaggtcgcc ggccacgagc gcaaagagat agctcggctt
     1861 gggaaacgga tcctcccagg tcacccggcg gcgcccttcg cccgccggga cgtcggagat
     1921 ggcatttccg ttggcgagca ggaccgggta gagcgatgcg tctgcttcca gggtcgtggt
     1981 gaagcgcgcg agaacgtcgg gacgatcctg atagtaagtg atccggcgga acccctcggc
     2041 ttcgcattgg gtgcagtaca tgctccccga tttgtataga ccttccaacg ccgtgttctt
     2101 ttccggctcg atacgggtca cgatcgtgat ctcgcagcgc tccggcaggt cgaagagggt
     2161 cagcgactcc gcatcgacca cgaactcgtt gccacccagc aaccggccat ccagggccac
     2221 cgagagcagc tccaggttct gaccatccag ccgtagcacc gtgtccgtgc cgcctgcgcg
     2281 gcgctccacg ctcagctggc tctcgacgcg cgtgtagcca tcgcgtacgt cgaagctcag
     2341 ccgggtatcc agggtccggt aagacggcgg ctcgtagtcc cggaggaaca cgggttgagg
     2401 gacgttgcta ctcatcggca aagctccata tgcaaccgtt gacggcctcg cgccgatcag
     2461 cgaccggagc cgaccagacg acccgtgacg atgccgagaa ctgctccggc ggcgagtccg
     2521 aaccaatggg ccgcgttggc cacgtgaaga ccgaaatact cggtcacgcc cgtgctgaac
     2581 agcaccagga agaacagcag cccacccgcg acagcggttg gcacctgcca ttgagacacg
     2641 gcgggcgcca gacggccggc cgccaggaca aagcccaaca ggccgaaggc gacgccggaa
     2701 agcccgccga acaggggatt cgcgttcatc gcgtactgcc cgagattggc cacgagcgaa
     2761 accacgagga cgattgccca gaaccagagt gccccaagcc gactttcgat ccgccggccc
     2821 atctcgatga cgacagcgca gttgaacaga agatgcacgg cgttgaagtg gatcagcatg
     2881 ggcgcgaacc agcgccagac cgcggcctcc gccaccagct cgaccagcgt cccgggagcg
     2941 gatccaagcc gaatcagatc cacgatcgtc agccagctgg caacatacgt tacgcggcct
     3001 tcggccagcg gcatggtaaa cggaaacagc gccacggcca gggcgaccag gaccgcaagc
     3061 cccgggtacc tgcggatctg cgcgagcagc cccggacggc gctgcggcag aggctgtgcc
     3121 tcgacgacca gagaacccga agccagcgcc tcgtagacgt cccgaaccaa tgcggcctgg
     3181 gcaccgtccg ccagctcgac gatctggcgc ccgcgttcct cgtagatgcg atgacgcacc
     3241 ctgcgttccc agagcaggcg ggacagcacc gagagatctt cgtccagatc cgtctcgatc
     3301 acgcgcatga agcacagcca cagtggtagt aacatgcaag cctaccaaat cggcgcaacg
     3361 gacgagatgc acgcagtaga cgaggaagtt ctcaatcagg tgaccgagtg gctgctggcc
     3421 gatcgcccct gctggctggc gaccgtcgtg gccacctggg gatcctctcc ccgccccgtc
     3481 gggtcgttgc tcgcgtgcaa tgccgaaggg caaatcgtgg gatcgctgtc gggcggttgc
     3541 gtcgaggacg accttctcga gaagctcacg agcggggagc tcgctgccga gcacgcgcag
     3601 acattcgagt acggtgtcac cgcggaggag tcggagaagt tcggcctgcc gtgtgggggc
     3661 tctctgctca tcgtgatcga gcccgtggct gcgaacgccg cctacaaagc ccacatgtcg
     3721 cacatccgtg accagctcgc tcagcgccgc tgcgtgaaac gaacggtgga tctgaaaaat
     3781 cgctccttcc ggaccttgca agtgccccag cacgagccgc tgcgctggga ggccgattcc
     3841 cagattctcg agcacaccta cggcccccgc taccagcttt tcgtcatcgg tgccggcatg
     3901 gtgtcgaagt acctcgcgga gatggcccag atgctggact accaggtcac cgtctgcgac
     3961 ccgagggaag atctgctgaa cgacttcggc atcccgggcg tgaagctggt ggccgacatg
     4021 cccgacgacg cgatcaaggc ccatgccagc gatcccgctt cggcgatcgt cgcgttgacc
     4081 cacgacccgc gaatcgacga catgggcatg atgcaggcgc tgaccaccga cgccttctac
     4141 gtgggggcaa tgggctccgc gcggacgtcg gccagccgtc gggagcgact cgccgcgctg
     4201 gagctgagcg aggagcagat cggacggctg cacgcgccca ttggcctgcc catcggcagc
     4261 aagacgccgc cggagatcgc cgtcgccatt cttgcggaga ttaccgccgt gcgtaaggcg
     4321 cgggccgaat ccgcctgcgc acacctggcc cagctgagcg ccggctgaat cgacgtgcgc
     4381 ggtggtgtgg tgctgctggc cgcgggttcc gccagccgct tcggctccga caagagaacg
     4441 cattcattgc ccgatggccg ctcgctgctc ggcgccacga tagccaaata cgccagagca
     4501 ttcgccgaga taatagtggt tctcgggccc gcggaccgcg cccttgccga agagttgcga
     4561 cggcagttcg atccgcagac cgtgaccatc gtccacgcgc aggctgcgcg tcttggcatg
     4621 ggtcattccc ttgcggccgg aatccgcgca gccaggggat gggatttcgc cttcgtggct
     4681 ctcggcgaca tgcccttcgt ccagccacag accctggcgg ccttgggccg cgccatgtcc
     4741 gaggcagggc acgacgccat cgttcagccg tttcacgaag gccacaaagg tcatccggtc
     4801 gggttcggcc gccggcactt cagcgcgctt gaagcgctca ccggcgacga aggcgcccgc
     4861 gaagtggtga agacgtcagc cagtgatctg gttcccttag aggtcgacga ccccggcgtg
     4921 ctgcaggacg tggacactcc ggccgatctc gccggggact gatcagccgg gcggttcagt
     4981 cgttgttccg gccgcccagt cgggtcgccc agtccagctt gctgcgacag gactcgtaga
     5041 agctgtggcc cagggtgtag accagccgca gagggtggtc gaacttcgat atgcatacct
     5101 cgtcgccgga gcgcagcgcg aaatccacct gtgaatcgca ggtgacctcc gcatcttcgg
     5161 ccgcgaggat cttgatctcc gactctcccg tcaccaccag gggtcgagac gtcagcgtgt
     5221 gtggaaacat gggaaccacc acgatggcgt caaggttcgg atgcatgatg ggaccaccgg
     5281 cggaaagcgc gtaggccgtc gagcccgtcg gggatgcgac gatgagcccg tcggagcgtt
     5341 gatcgtagac aaactcgccg tccacccaga gcgagaactc catcatgcgg gccataccac
     5401 ccatgtggac gaccacatcg ttgagggcgg gcgaatggcc gagcgtctcg ccagcccgga
     5461 cgatgcgggc gttgaggaga aagtgatctt cgatcacgta gtcgcctttg aggacctcgg
     5521 ccagcttctc agcgatctga tccggagaca cgtcggccag aaaacccagg ccaccacggt
     5581 tcacgcccag cacgggaaca cgagcgtggg ctagatccct gcccaccccg aggaggcttc
     5641 cgtcgccccc gaccacgatg atgaggtcac agttttctcc cagagcctga cgggacacct
     5701 gctcgtggcg cagtttcttt cccattctcc gcgcagtgtc cgattcgagc agaacgcgcc
     5761 taccgttggc ctccatgcag tcgatgacgg cacgaagact gtcgcgtatg cggggtttgc
     5821 ccgaacgacc gatcaggccg acagtctcga aagaaccggt catgacagtc cagagtgaag
     5881 gaaaaccccg acactacaca cgccgccggt tgccgacaat gcttgcaata ccgtcatgaa
     5941 tacatagtct gaacggtcat catgaccagc gcactggatc aacgttactc tcaggtcctc
     6001 accgaggctt taagccagtc ggcgcacgga gccggcgagc cggacgacct gtggactctg
     6061 atgcgcggcg aagctcactc gaaagcggcc gaggaaccca tactcggcag ctacttccac
     6121 gcgacgatcc tgaatcacga aagcttcggc tccgccctca gcttccggct cgctagcaag
     6181 ctcgacaatc ccatgcttcc gaccatgctg attcgggatg tcatacagga ggccatggca
     6241 gacgaccctg cgatcctgcg gtcagcggag atcgacatac tggcgacgta cgccagagat
     6301 ccagcctgca ccgatctgtc tacaccgttt ctcttcttca agggctttca tgccctgcag
     6361 gctcaccgca tcgctcactg gctctggctt caggggcgcc ggacgctggc ccagttcttt
     6421 cagaatcaga tcagcgtgac gctcggcgtc gacttccacc ctgcagcacg catcggctgt
     6481 ggcgtgatgc tcgatcacgc cacgggaatc gtggtcggcg agaccgccgt ggtggaggac
     6541 gacgtctcca tattgcatgc cgtcaccctc ggcggatcag gaaaggtcag cggggaccgg
     6601 cacccgaaga tcaggcgcgg cgtcctgctt gccgccggat gcaaggtgat cggcaatatc
     6661 gaggtgggtg aaggagccaa ggtcggggca ggcagcgtgg tgctcgagaa cgtgccgcct
     6721 cacgtgaccg tagccggcgt cccagcccgc atcgtcggcc agaccgacga gggcaccgtg
     6781 ccggctctgg acatggacca gcgactcgtc tgacccagag ggggacgcca tggcagagcc
     6841 ttgctgtcca acctacggtg tcgaaggcgt cggccgcgcc tacaacgtcg ttgccaagca
     6901 tgcgccagct tggcaatgac ggggccttcg cggctccagt tccagcatgc gcacctgacg
     6961 atacgccgcg gacgccaatt cggtagccaa ttcgcctaac gggcatcgtc ttcgcgagcc
     7021 agaaccgccc gagactcccg ccgtcctgcg acggctgcgt gttcgagcgt ggtggggatt
     7081 cactacagat cccagatctc gattgcgggc gaccccatat gacgctcgac tcatgattgc
     7141 atgatcgaac ctgctgcgtg gtacccagat ggatctggcc tgcgtccttc agtccccgac
     7201 caccgaaagg ttgacgccgc cgaggtcgcc gaatctttct tgcacaactt ccagcgcgtt
     7261 cgcaggcttt gccgacatgg cgtcaccgga gcgcactcga tacgctcccg gagattccgc
     7321 gccgcgtggc ggccgcccct gtcgcaccgc gtcgaccgcc tcgaacatca gttggcggaa
     7381 tcgaaccacg cccaggtcgg tttgacccag aagctcacgg gtgcggtcgg caatcaatcc
     7441 ctggctgtcg gcgatggcgg cgtcctgctc ggatatgccg gtgatgccgg tgaaactgcc
     7501 gtatcgctgc agattccggt cgatcaggta gtcgttgccg cggttgcgca ccggcacgaa
     7561 ctcatcgtcc acctcggaga aaataccgaa cccagacgcg aatgcgtgcc gctcccgatc
     7621 gctcagcgga cgctccgggt tgtaggcgta gcaatagatc caacaggagt ggtcgtcgat
     7681 tggaacccag gtgttgccga catgattgtc ccccggaaag ctgttgggtg ccatggaatg
     7741 gttgggcatc atgaactgcg tgagacgcca atagagctga tcgtcgtccg ctttgcgggc
     7801 agcacacatg accaggcccg cttcatgctc gagcacggtg aagcgaggcc ttgaatcctc
     7861 gatcatccac cggtagcggc cgagctcagg cggctcgttg gcacccgtgg acggcatcaa
     7921 accggcgcgt tcgccgcctt cgatgttggc atgcagatac gagaagtggg ccgtgtcgat
     7981 gccgccctcg gcagcctgtg cccagttgca ctcttgcagc ttcttcgaca cgaaataatt
     8041 cgcttcgggc aagcccatga aatcgaactg cgggacgtcc ccgccgccgc caccgaagtg
     8101 cacccagacg atgccacagg attccttgac cgatagcgcc cgcagcgcgg cccttggttt
     8161 gatgctttct gcaaccttgg cgtccgcggt cggtacgtcg acgcaccggc cgctcacgtc
     8221 gaactgccag ccgtgataga cgcaacgcag gccttcaagt tcgttgcgcc cgaagaagag
     8281 atgcgcgcca cgatgtggac aattgggctc gaccacaccc acctcgccgc gaccgttacg
     8341 aaatgcgaca aactcctcac cgagtatctt cacccgcttc ggcgcgcctt ccgcctcgat
     8401 ttccctggat aaaagcaccg gttgccagta ggcgcgaaat acccgtccca tatccgtatc
     8461 ggggccggtc aacgtcagca cctcgttctg cgccgcggtc agcatttcct ctctccccat
     8521 aacgcctcgc tgttcaattg tgccactatt tcgctgatct catcgacatc gatcccgagg
     8581 gctccaactg aatgacccac ccactttccg cagcggttct gcgatcgtcg acactgggcc
     8641 tgtcccgggc gtttgtgcat ctggccgcgg aatacggatg gtacggttac cgccgtatca
     8701 cggcgctctt gaaccagcag ggctggacgg tgaaccacca gcgggtggag cggatctggc
     8761 gttgagaggg gctgaaggtt ccccagaaac agccgagaag agccgtcagt gtctggcgat
     8821 ccgcacccag cgcagatccc catcgagcgc tggcgcaaac actgcaatct cgaacggcga
     8881 caaagtgact cgggtcatcg actaccggca ccgcaagcca aaacatctga aaccaaccag
     8941 cgatgctcct acgggcaccc actctcagaa ggtcgcgcag gtcgctacgt tgtcgtctgg
     9001 cgagtagctc ggggcgtcgg gacctactac cacgtcgatg acacagaaca tgaaggagac
     9061 gccacccttc acgctgtcgg tgctctgaaa ggcggcaagg ccggcactgt tggtcggcgt
     9121 ggtctgctct tcgttgtaat cgccggaaaa gatcgctctg acatccgcat tttcaactgg
     9181 ctggccgcag tcgttctcaa tcacaacctg ggcctgggcg cgttttgccc ctcggtctgc
     9241 cttcacggtg aacaccgaga tggcggccac gtgaaacgac tcggcgggtt cgcagagcgg
     9301 cgggtcgtgt tcggtcgtcg cgctggcaat ccctgagggt tgggtctcgt tccgggctgg
     9361 gctcttgtcc cgagcggtca cctggaaatc gtactctgtc gccggaagca agcccgaatt
     9421 ctcgtgctgt cgtgagtcct gccagccact gtcgatgcac ccgaggcccg ggatcacaca
     9481 agtgaaattg tactcgaccc cgcttggatc actggccggc accgcggtca tggcaatgga
     9541 ggtaggcccg gtagcatggg gcgggatctc ccaatccatg ttcgcgggcg tgggcggatc
     9601 caggtcggga gaggacgcca cctctagctc ccggatggga gaggctggca atcccgccga
     9661 gttctggagg ttggggttgg ccggggcggt ggcataggca tagcgggcga agactgggac
     9721 cgggacctct ggatgggaaa gcgtcacccg gccgtccccg gttagcaccg cctctgccgc
     9781 tttatagttc ccgtcagcgc ctgcgatttc ccattccgcc ggagctcggc tgtcgctcgt
     9841 ggcaaggcct tgctcgacgt gccggaagcc aacgtacaac acgtccgcaa ccacttcgga
     9901 caacgtcggg tcggcgacgg ggccgaaggc aacgacatcc tggccgtgga cgttccgtag
     9961 ggcgatgttc gccaggcgct ccccgaaggt ccggtattcc gagggatgga cgtcaacctc
    10021 atgccagtcg acgttcacgg tcatctccac gtccaccccc acgagggcag tctgcaactc
    10081 cgcgtcccgg atgacatgga tggagttgat tcccatgcgg ggcatctgca ccacgtagaa
    10141 ggggaaattg ccctggcccc agtccgcccg ccactcctcg atcagggccg gaagctgcca
    10201 atagtattta ctcgagtcct gagcgccgcg ggcgtcccac tccccttggt accagagaac
    10261 gcctcgaatg gcgtagggct ggagaggctg aatctgctcc tgatacaggc cgccactccc
    10321 tgccaccgtg gaccagcgct caatcgccgt agcaccgacg gaagaatgaa tgaggccgat
    10381 cggcaggccc accagctccg ggtccgaagc caacgcccgc tccaagcgtc tcccgaacca
    10441 gaagagcacc gccgacatgc tctctgcggt ggcaccgttg gacggctccc agagggcgtt
    10501 ggacggcgtg ccggaggcca cgttgaagaa agagaggttg aggtgcggtg cgtccgcgag
    10561 accgaggccc gcctcaccgt cgccggaaag cgggcggttc atgttggact gaccgctacc
    10621 cagccacacc tcgcccacac gaacgttgtt gattgtgacg ctcgggaatt cgttctccag
    10681 tacagtcagg ctgtggtact cgaggtcgcc ttgcagaggg ccgatggacg cttgccagac
    10741 accgcttgca tcggcaacgg tcgtggccac agggttttcg tcgtcgtccc agagaatctc
    10801 gatcttggcg cctggagccg aagtgcccca aaccggcacc ggcatatcgc gctgcagcac
    10861 cgcgtggtca ctgaagaggc tcggcatgct cacctcggcc ggtgccgagg ctgggcctat
    10921 cgccagggcg accaataatc ccgccagggg gattcgatcc atggctgtgt tacgcctcca
    10981 ctgcgcgatt caccgacagt acacttacgc atttttattg tctataaata cgcgggcttc
    11041 ttccggcatt cccaccaaca cgaggacatg aaagcctcca ccgaagt
//
LOCUS       DBNHLM010000016         2482 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_61603, whole genome shotgun sequence.
ACCESSION   DBNHLM010000016 DBNHLM010000000
VERSION     DBNHLM010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_61603"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            425..>2482
                     /locus_tag="AC16QF_00930"
     CDS             425..>2482
                     /locus_tag="AC16QF_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6447142.1"
                     /translation="MTATRRAETEIQTTPVAVSVVSEEEFDKLYAQDIGEIALYVPNF
                     SAATVTGFNAASFAMRGAGETDIIVYFDPKVGVLVDDFVIPHVQTQLLEPFDIEAIEV
                     LRGPQGTLFGKNTTSGAVVVRTKRPELESTFADVSLQYGRFDDVKANFAVNLPLGETA
                     AFRFAGLYQKSDGYYENGKVDNPNSFGGGVGDAADADGSPVAGDGSKTGGKDIFSGRA
                     KLLFQPNDQLTLLGQLELIKDRSDPVPVVNTTPAAAGQVATIFGFPGVENGNPLKQAG
                     IHESSIVGLDGDQEVDVFGAYLNLEYALDNHTIYGLIGYRDQESRLPSEYLGTPYESF
                     FAATRDDDRETFQLEARIASDREDRLNYVVGGFYQTNDADFCVLQQLGLLEFFGPQSA
                     IPGVLDNDNPLLLCNRQNAESWAVFADVTIDVTDRFQISLGGRYTDEEKDYIARQGLP
                     VSFIFPNGQFDEPLDGQDFDLGNFPIGGGLNSVETDKESWSEPTWRFTASYRFTDDVF
                     GYATVSRGFKSGGYNDQAGSGGFQNFPVAAYDPELATSYEVGIKTNLAEDRVRLNATY
                     FFVNYEDFQRSTVVSVPGTAFQETRTFNAADVDAQGIELELSALLAENLTLRANLGWI
                     DAEYNEFLLDRDGNPDTPPTDLSGRDVVRAPEISAGIDLTYDYSLGASGGLRFNVSFL
                     YEDE"
BASE COUNT          472 a          728 c          775 g          507 t
ORIGIN      
        1 ccgcggtaca tgaccagctc gcctacgtag ctctgcaatc cgaccatcgg tggttatcca
       61 gaaaatgacc cgaatattgt gatccgtttc tcggtttgtg gtgtgagtaa gcgtaagcgg
      121 tatgtcaatc ggatatcgaa tcgcgtctac gccgagcatt gcgagggcgc gaaattttgc
      181 gcggccagac cattggattg ctggacactc gcgcttgatc tcagctggca attgccggag
      241 catggttgcg ggataaagtc gcgggataaa ttggagagtg gatatgtttg tgcgttcagg
      301 agtcgggttg cgaagtctcc ttcaaaacct gacaagaggc tttaaattgg ggctgttggg
      361 cgccttgctt tctctgccgg ccacgtctgc gttcgctgcc gacggggttc tcgaggagat
      421 cgttgtgacc gccacacggc gtgcggagac cgagatccag accacgccgg tggccgtgtc
      481 cgtggtgagc gaagaggagt tcgacaagct gtacgcccag gacatcggcg agatcgcctt
      541 atacgttccc aacttctcgg cggccacggt gacgggcttc aacgcggcgt cctttgccat
      601 gcggggcgct ggggagacgg acatcatcgt gtacttcgac ccgaaagtcg gcgtcctcgt
      661 ggacgacttc gtgattcccc acgtgcagac ccagctgctc gagcctttcg acatcgaagc
      721 catcgaggtg ctcaggggcc ctcaggggac gctgttcggg aagaacacca cctcgggtgc
      781 cgtagtggtt cgtaccaagc gaccggagct cgaatcgacc tttgcggacg tttccctgca
      841 gtacggtcgc tttgatgacg tgaaggcgaa cttcgccgtc aacctgccgc tcggggagac
      901 tgccgccttc cggttcgctg ggctttatca gaagagcgac ggctactacg agaacggcaa
      961 ggtggacaac ccgaactcgt ttgggggagg ggttggtgac gccgccgacg cggacggctc
     1021 accggtcgcc ggtgatggtt ccaagaccgg cgggaaagac atcttctcgg gccgcgcaaa
     1081 gctgctcttt cagccgaacg accagctcac gctgctcggt cagctcgagc tgatcaagga
     1141 tcgcagcgac ccggtgcccg tggtgaatac caccccggcc gcagcgggtc aggttgcgac
     1201 gatcttcgga tttcccggtg tggaaaacgg caacccgctg aagcaggcgg gcatccacga
     1261 aagctccatc gtgggtctcg atggtgatca ggaggtggac gtgttcggtg cgtacctcaa
     1321 cctggagtac gcgctggaca atcacaccat ttacggtctc atcggctatc gggatcagga
     1381 gtcccggctg ccgagcgagt acctcggcac gccatacgag agcttcttcg ccgcgacccg
     1441 tgacgacgac cgcgaaacct tccagctcga agcccgcatc gcttcggatc gggaagaccg
     1501 acttaattat gtggtcggcg gtttctacca gaccaacgat gcggacttct gcgtcctgca
     1561 gcaactaggt ttgctggagt tcttcggccc ccaatcggcg attcctggtg ttctggacaa
     1621 cgacaacccg ctgctgctct gcaatcgtca gaatgcggag tcctgggctg tctttgcgga
     1681 tgtgaccatc gacgtgaccg accgctttca gatcagcctg ggcgggcgct acaccgatga
     1741 ggaaaaggac tacatcgcgc ggcagggact gccggtgtcg ttcatcttcc cgaatggaca
     1801 gtttgatgag cccctggacg ggcaggactt cgatctgggg aactttccca tcggtggcgg
     1861 cctgaactcc gtggagacgg acaaggagag ctggtccgag ccgacctggc gtttcacggc
     1921 ctcctatcgg ttcaccgatg acgtgttcgg ttacgccacg gtctcccggg gattcaagag
     1981 cggcggctac aacgaccagg cgggctccgg tggcttccag aactttcctg ttgcggctta
     2041 cgatccggag ctggcgacga gttacgaggt cggtatcaag accaacctgg ccgaagaccg
     2101 cgtgcgcctg aacgcgacct atttcttcgt gaactacgaa gacttccagc gcagcaccgt
     2161 ggtctccgtt cccgggacgg cctttcagga aacgcgtacg ttcaacgccg ccgacgtgga
     2221 cgcccagggc atcgagctgg agctttccgc gctgttggca gagaacctga ccctgcgcgc
     2281 caacctcggc tggatcgacg ccgagtacaa cgagtttctg ctcgatcggg acggcaatcc
     2341 ggacaccccg cccacggatc tttccggtcg ggacgtggtt cgtgctcccg agatctctgc
     2401 cgggatcgac ctgacctacg actacagtct gggcgcgagt ggaggccttc ggttcaacgt
     2461 cagcttcctc tatgaagacg ag
//
LOCUS       DBNHLM010000017         5868 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1378799, whole genome shotgun sequence.
ACCESSION   DBNHLM010000017 DBNHLM010000000
VERSION     DBNHLM010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5868)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1378799"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="AC16QF_00935"
     CDS             complement(<1..269)
                     /locus_tag="AC16QF_00935"
                     /inference="COORDINATES: protein motif:HMM:NF037688.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447143.1"
                     /translation="MDRLAELFARVADSGVCTAATLREKFLACGIQPDDFRLRRTWQG
                     LDELAERPIDLETFRQLVSPELLMVARVLNKRLIVPEWQDFRQDI"
     gene            complement(1126..1890)
                     /locus_tag="AC16QF_00940"
                     /pseudo
     CDS             complement(1126..1890)
                     /locus_tag="AC16QF_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700584.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(1894..2835)
                     /locus_tag="AC16QF_00945"
     CDS             complement(1894..2835)
                     /locus_tag="AC16QF_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6447144.1"
                     /translation="MNLLIGALTLGLILGILALGVYMSFRILHIPDITAEGSITLGAA
                     VAAALIASGTHPAVATLIAALAGFVAGTGTGLMTTKCRIQPLLAGILAMTALYSINLR
                     IMGRSNIPLINETTLATYVGRFGDALAGGGSGVNILGWTVGIRDLSILVGAFVVVVVI
                     SLFLYWFYRTQLGTAMRASGDNPQMMGALGVSVETLTILGLAMSNGLIALSGALIAQY
                     QGFADVQMGIGIIVIGLASVIIGEALVGARTVGLLLCGAVMGSVLFRLLVAIALRWGL
                     DPNDLKLVTAVFVFIALMAPTLIRRFGPSARLRSAGA"
     gene            complement(2839..4872)
                     /locus_tag="AC16QF_00950"
     CDS             complement(2839..4872)
                     /locus_tag="AC16QF_00950"
                     /inference="COORDINATES: protein motif:HMM:NF016292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HIR6447145.1"
                     /translation="MVNSTSVLGRLSFGLIAIAAAAAILLFSDLEARKDRTVSDADDA
                     AKVWRVALVQLAPVQPVDEGVRGILAGLAARGYVDGDRLEVIQYNAQGDLAVANAIAA
                     QVTSAGLDMIITASTPSLQTVANANRSRANPTVHIFGISSDPYGAGVGISREDHLDHP
                     PYMAGLGSLPPVRELFDLVRTINPAARRVGLVWNPTEANSEAATHLARAAAAEFGMEL
                     VEGNAESSTAASEVAQSVISRGIDVLWVSPDSTVYTAIDALIEAARRGGVPVVTSLPG
                     SVGRGSLLDLGARYEDIGYVQGLLAADVLEGRDPASVPVENWIPVDLHLNLTVLDGIS
                     RSWRIGPDLVASASVVIDDSGTRERGLPKPEPPEWLRWDRLAEGGQVPNVDLVMYSES
                     PVAEQSIEGVRLGLAQGGFDPDLRLNLRVHSAQGDMPTLVSIIDAIRPTSTELIMTLS
                     TPALQNALQRVRGIPIVFGMVSNPFIVGAGISDTDHVPTATGAYLNQPLESALDIVAE
                     LFPEARTLGTLFTPAEVNSQYNRDRLLEVAKARGYSVENIGIATAADVLDASIALAAT
                     RPDVWVQVVDNLISSSYPAVMEAADRERIPVITFSVTAAEFGPLVIVGRDYFDAGVEA
                     GRLAARVLRGESVAALPFRPASTTRYILNHEAARRFGVRFSDELIGLANAQGH"
     gene            5263..>5868
                     /locus_tag="AC16QF_00955"
     CDS             5263..>5868
                     /locus_tag="AC16QF_00955"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6447146.1"
                     /translation="MAHAKPLVVDMDSHVLEPPDLWQRYLEPKYRHRAIRIEQGADGI
                     ETLFVDDKALLSGRLAALGGAEHDAVRTFTDPDLTYLDGCPKASYDTQARVQLLDEWG
                     VDMGVVFPTIGILWDKEDDPELAMAYARAYNNWQWDFAKPALDRILPIAHVPLYDVDL
                     AYEELKRCLKLGFKGMFMAPEPVCGRRPSHPDFDPIWQLLID"
BASE COUNT         1081 a         1948 c         1845 g          994 t
ORIGIN      
        1 tgaatgtcct gccggaagtc ctgccattca ggcacgatca gccgcttatt cagtacacgc
       61 gccaccataa gcagctcggg gctgaccagc tgccggaagg tttcgagatc aatcggacgc
      121 tccgccagtt cgtccaggcc ctgccaggtc cggcgcagac ggaaatcgtc cggctggatc
      181 ccgcaggcca ggaatttttc tcgcagcgtt gccgccgtgc agacccccga gtctgcaacg
      241 cgggcaaaca attcggcgag acgatccatg aaagcgcctc acatctgaga aaggcagtaa
      301 aggccctttt ctgagttggc gcaaacacct tccagctcag gctgccaatg gtgtcaggat
      361 tccaaggctg aatccagcag gtaatctggc tttgcacggc gcgcgcaggc accattcagc
      421 tccagcctca cttcgattct gaaatcgcga ccgggagcag catttacgtg cgttcgggag
      481 gtggtcagct ttccgaattc ctcggcctgc cggcacccct tggatttgcc caggggccgg
      541 ggctcagagc tcgatgcgcg tggtgaagaa gcctttggcc acgtagcctt cgcacaccat
      601 gtcggcgtac ttcgccgaga agaaacgccc cacggagata accttgccgc cccgcatgaa
      661 ggtgggctcc gcttccggga ccaacgcgcg cgcctccccc acctgcgctt cgttttgagg
      721 cacgatgacc acctgatgcc tgccctggtc gctcgagctg gtgacgtagc ggatgaccat
      781 cgcacccacc tcgtccatga ccgtcgtctc ttcctcggtg accgtctcgt ctgcgtgcac
      841 gaagtgctgg agcacgtcga gcagctccga ggcctgctcg gtgggcggag agacccgcag
      901 gtatgccgct acggcggcgc gcaccgccat gatgccctcg cccgtcacgg ggggagaagt
      961 gaggtccggc aggggaacgt tccaccgctc ggcgaattcc cggaggagct ccacctcacg
     1021 ctcgtccaca cgctcgtcca cgtaaatgac cgccttgaag acttccatca gctcctcgat
     1081 gccctgtgcc tcgaggagga gtccctccct cgaccgctcg agctgctcct tgcggcgaag
     1141 gtcctcgaaa cggctcagca ggtcctcggc tctgatctgc tcccgggcgg ccccggagat
     1201 gtcgtgcagc acctggccct ggtgcatcat cacgatccgg tcgcccagat ccaccgcctg
     1261 ctgcatggag tgggtcacca tcagcgtggt gatccgttcg cgcgcgatga tctcgtgggt
     1321 gaggcggatc acctgagccg cgctcttggg gtccaacgcc gccgtatgct cgtccagaag
     1381 aagcaggtcc ggcttaagcc aggtcgccat gagcagcgtg agggcctggc gctgcccgcc
     1441 cgagagggtg ccgatggggt tgtccaggcg attctccagg cccatgttca gaacgcgaat
     1501 acggtcccgg atctcgtccc gaacgccggt cgtgagagcc ggcgccaacc ctcggctctg
     1561 cccgcgacgc gctgcgagcg ccaggttctc cgcgatcgtc atggccggcg cggtacccga
     1621 gaaggggttt tgaaacaccc gcccgaccag gcgggcgcgc cgatgctcgg gccagtcgct
     1681 gatcgtctcc cccgaaaggt ggatcgagcc cgagtccacc ggaaaggagc cggcgacggc
     1741 gttcagaatc gtcgacttcc cgctgccgtt catgccgatg atgatcagga acgacccatc
     1801 ggcgaggtcg atggaaacgt ccgtcagagc gcgcacctcg ttgctggtgc ccgcgtgaaa
     1861 cgtcttcgag atcttctgga tgctgagcat ctgtcaggcc cccgcggacc gcagtcgtgc
     1921 cgacggcccg aagcgtctga tcaacgtggg cgccatcagc gcgatgaaca cgaagaccgc
     1981 ggtgaccagc ttgaggtcgt tgggatcaag gccccaacgc agcgcgatcg ccaccagcag
     2041 cctgaagagc accgaaccca tgacggcgcc gcacagcagg aggccgaccg tgcgggcacc
     2101 cacgagcgct tcgcctatga tcacgctcgc aaggccgatc acgatgatgc cgatccccat
     2161 ctgcacgtcc gcaaagccct ggtactgggc gatcaaggcg ccgctcaggg cgatcaggcc
     2221 gttcgacatg gcgagcccga ggatggtgag cgtctccacg ctcacgccca gggcacccat
     2281 catctgagga ttgtcaccgg aggcgcgcat ggccgtaccg agctgggttc ggtagaacca
     2341 gtagagaaac aggctgatca caaccacgac gacgaaagcg cccaccagga tcgaaagatc
     2401 ccggatcccg accgtccaac cgaggatgtt cacaccggaa cccccgccag ccagcgcgtc
     2461 tccgaacctt cccacgtagg tggccagggt cgtctcgttg atcaggggaa tgttgctgcg
     2521 ccccatgatc ctcagattga tcgagtagag ggccgtcatc gccaggatcc cggccagtag
     2581 aggctggatc cggcatttcg tggtcatcag ccccgtgccc gtgcccgcga caaacccggc
     2641 cagagccgcg atgagcgtag cgaccgccgg gtgggtgccc gaggcgatca acgcggccgc
     2701 gaccgcagcg ccaagggtga tcgacccctc cgcggtgatg tcgggaatgt gcaggatgcg
     2761 gaaactcatg tacacgccaa gggcaagtat cccgaggatg agtcccaggg tgagagctcc
     2821 gatgagaagg ttcatggttc agtgcccctg tgcattggcg agcccgatca gctcgtcgga
     2881 aaagcggacc ccgaagcggc gcgcggcttc gtggttcagg atgtaccggg tcgtcgatgc
     2941 cggcctgaac ggcagagcgg ccaccgactc gccgcgaaga acgcgtgcgg cgagcctccc
     3001 ggcctcgacc ccggcgtcga aatagtctcg cccgacgatg acgagcggcc cgaactcggc
     3061 tgccgtgacg gagaatgtga tcaccggaat cctttcccgg tcagcggctt ccatgaccgc
     3121 ggggtagctc gacgagatca ggttgtccac gacctgcacc cagacatcgg gccgcgtggc
     3181 tgcgagcgcg atcgaagcat ccaggacgtc tgcggccgtg gcgataccga tgttctccac
     3241 cgagtagccc cgtgcctttg ccacttctag aagcctgtcc ctgttgtact gggagttgac
     3301 ttccgccggc gtgaacagcg ttcccagcgt ccgggcttcg gggaagagct cggcgacgat
     3361 gtccaacgcc gactcgagcg gttggttcag gtaggcgccc gtggccgtcg gcacgtgatc
     3421 cgtatcgctt atgccggctc cgacgatgaa ggggttcgac accatcccga agacgatcgg
     3481 gatcccgcgg actcgctgca gcgcgttctg gagcgccggc gtgcttagag tcattatcag
     3541 ctccgtgctc gtgggcctga tggcgtcgat gatgctgacg agggtcggca tgtccccctg
     3601 ggccgagtga acgcgcaagt tgagcctaag atcggggtcg aagcccccct gagcaaggcc
     3661 cagcctgacc ccttcaatgg attgctccgc aaccgggctc tccgagtaca tgaccaggtc
     3721 gacgttcggc acctgccctc cttcggcgag ccggtcccag cgcagccact cgggcggttc
     3781 gggcttaggt agccctcgct cccgcgtgcc ggagtcatcg atcacgacgc tcgcgctggc
     3841 cacgaggtcg ggtccgatgc gccagctgcg ggagatcccg tccagcaccg taaggttgag
     3901 gtgcaggtcg accgggatcc agttctccac cgggacgctc gcaggatccc gcccttcgag
     3961 gacgtcggcg gcgagaagac cctgcacata gccgatgtcc tcatacctgg cacccaggtc
     4021 caggagggag cctcggccga ccgaacccgg cagggaagtc acgaccggaa cgccgccgcg
     4081 acgggcagcc tcgatgagcg catcgatggc agtgtagacg gtgctgtccg ggctcaccca
     4141 gaggacgtcg atcccccgcg agatcacgga ctgcgccacc tcgctggccg ccgtcgatga
     4201 ctccgcgttc ccctccacga gctccatccc gaactcggcg gcggcggcgc gggccaagtg
     4261 ggtggccgcc tcggagttcg cctccgtcgg gttccacacg aggcccaccc ggcgcgcggc
     4321 aggattgatg gtccgcacca ggtcgaacag ctcccgcacg ggtggcaggc ttcccaggcc
     4381 ggccatgtag ggcgggtggt ccaagtggtc ctcacgactg ataccgaccc cggcgccgta
     4441 gggatcggaa gagataccga agatgtgcac ggtcggattc gcccgggacc ggttggcgtt
     4501 cgccacggtc tgcagcgagg gggtcgaggc cgtgatgatc atatccagtc ctgcgctggt
     4561 gacctgcgcg gcgatggcgt tggcaacggc caggtcgccc tgggcgttgt attggatcac
     4621 ttcgaggcgg tcaccatcca cgtaaccccg cgccgcgagc cccgccagta tcccccggac
     4681 cccttcatcc accggctgca cgggggcgag ctgcaccagc gcgacccgcc acacttttgc
     4741 ggcatcgtca gcgtcggaaa ccgtccggtc ctttcgggcc tcgaggtcgg aaaacagaag
     4801 gatcgcggcg gccgccgcga tcgcgatcaa cccgaaagag agcctgccca acacgctcgt
     4861 ggagttcacc atcgagaatc tacacttccc cccaacctgt ataagacatc ttacgttggg
     4921 aaaacaaaag caaaaaagct ctggttgcga gaagaagggc ccgcccggct accagcccag
     4981 catcgagtac atcacgcacc gggcttccgc ggaggccgtc atggccttct ttccgggcct
     5041 gacgcgcgac gggctcccag acggtgaggg atgggccgcg acgctgtacc tcctcgagcg
     5101 cggcggccgc gtcaccgcca ccgatcccca ccgaagcccc gcatcggatc gcagcgatca
     5161 gctcgacacc ctcggccctt cgaccgattc aaggcgaatt ccgccaacct cgccagatac
     5221 gggcgttata ctttcgcgaa cagccagtga tgggagaaac atgtggccca tgcaaagccg
     5281 ttggtagtgg atatggactc ccatgtgctg gaaccgccgg atctgtggca gagatacctc
     5341 gagcccaaat accggcatcg ggccatccgc atcgagcagg gagccgatgg catcgaaacg
     5401 ctcttcgtgg atgacaaggc tctgctttcc gggcggctcg cggcgctcgg cggcgcagag
     5461 cacgacgccg ttcggacctt cacagacccg gatctgacct acctcgatgg ctgtccgaaa
     5521 gcttcttacg acacccaggc gcgcgtgcag ctgctcgacg agtggggcgt ggacatgggc
     5581 gtggtgttcc ccaccatagg aattctgtgg gacaaggaag acgatcccga gctggcaatg
     5641 gcgtacgcgc gcgcctacaa caactggcag tgggattttg ccaaaccagc tttggatcgc
     5701 atcctgccga tcgctcatgt tcccctgtac gacgtggatc ttgcctacga ggaactcaag
     5761 cgctgcctga agctcggctt caagggcatg tttatggcgc cagagcccgt gtgtggcagg
     5821 cgcccttccc acccggattt cgatcccata tggcagttgc tgatcgat
//
LOCUS       DBNHLM010000018        13398 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_959519, whole genome shotgun sequence.
ACCESSION   DBNHLM010000018 DBNHLM010000000
VERSION     DBNHLM010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13398)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_959519"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="AC16QF_00960"
     CDS             <1..542
                     /locus_tag="AC16QF_00960"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6447147.1"
                     /translation="IYEVTQIKGDGEAHPLLSPNDEFADYETWDQGNLGPNLKDDDML
                     QYEYAREALKKGLAHEVRLGVNPYKFGLQGATDSHTGLATAEEDNFFGKHSQTEPTAA
                     RLATDVGHFENNVWPGWYQVASGYTGVWATENTREAIFDAMMRKEVYAAKYFGTTAPE
                     ESPMTTQERAYTSPIWYTP"
     gene            602..2536
                     /locus_tag="AC16QF_00965"
     CDS             602..2536
                     /locus_tag="AC16QF_00965"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6447148.1"
                     /translation="MAGILTLLAACDPAGTEPTGTDTDAAPQPVTVGPAPSAMGDRRL
                     LWGDTHLHTSYSPDAYLMQNRSADPDTAYRYAKGLPVVHPYHKARIQIGTPLDFLVVS
                     DHGEFMGVVPMVLQGDPRVANTETGKRYRAMAEAGREIEVFGELIAQVNKIVPPDQDL
                     YNPDINRSVWGEIMEAADRHNEPGTFTAFMGWEWSSTPQGQNLHRVIVMRGDKDNGDE
                     FVPYTSLDSDRPEDLWAWLEETAEAAGTDFLAIPHNSNISGGLMFPLVNSDKEAVDAE
                     YAKLRMRWEPVVEMTQIKGDSETHPMLSPNDEFADYETYEHLIAAEGIEQQTMFTEGF
                     LGTLSDADRAVLESQEKRIVKNGDYARTGLLRGLVLEERGRGNPYKFGMIGSTDSHTA
                     MASAEEDNFHGKMAIDSTPGTKREDIIPGTPGWDMGAAGLAAVWATENTREGIFDAMK
                     RKEVYATTGPRIELRFFGGFDFAAADAQARDLASVGYGKGVPMGGDLTASPAGNAPSF
                     LVSAVKDPVGANLDRVQIVKGWVDAEGMPQEKIFDVALADGRTVGTDGKVAPVGNTVD
                     VSTGHYTNAIGDPQLTVVWRDPEFDPGKRAFYYVRVLQIPTPRHTLYDAIALGISVEE
                     TGHPATIQERAYSSPIWYAP"
     gene            2608..3507
                     /locus_tag="AC16QF_00970"
     CDS             2608..3507
                     /locus_tag="AC16QF_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR6447149.1"
                     /translation="MNKLLKEPLLHFLVLGLALFVLFDVVSDEKETSDSKLIRVDRET
                     LLTFIQFRTRAFEPSIAAQRLDGLSDQELKLLIDDYIREEALYREALALGMDKNDYVI
                     KRRMIQSIEFITRGFVTRAVEVSDDDVKAHYEANRNDYYVEPFVTFTHVFISGENRSE
                     AEVDALAAAELTELNARQIPFSAAPGRGERFPFFVNYVERDPEFVASHFGADMANEIF
                     ALEPDELVWRGPFLSTYGAHLVLLTRKAPGRFPPLGEIEESVRQDAERAALQKANETA
                     IQAIVDTYEVERQPEPRTRAGAG"
     gene            3504..4526
                     /locus_tag="AC16QF_00975"
     CDS             3504..4526
                     /locus_tag="AC16QF_00975"
                     /inference="COORDINATES: protein motif:HMM:NF025167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HIR6447150.1"
                     /translation="MRTLRSAVLGLWACCAALPAVGHDARPNYVQITETAPNSFNVAW
                     KVPASMPGNALPHPLLPDDCKPQQPPRWHTTGAEHAAQQEFICEQGLSGRVVGIEFPV
                     INPSLSTLYRVRLANGEEHLKILKPDETVWMVPDAEDRLSVAKEYTGLGIEHIWIGLD
                     HLLFVACLLFISRTPRRLLITITGFTVAHSITLALSALDLVRIPTPPVEAVIALSVVF
                     MAWEIVRGDDDSLAYRFPVAVSSSFGLLHGFGFAAVLRDIGLPQTELPTALLFFNVGV
                     EIGQILFVMALLIAFVALRPALGRALRAEPGGAVHWSYLTTPASYVIGTLASYWLIER
                     LAGFWG"
     gene            complement(4560..5828)
                     /locus_tag="AC16QF_00980"
     CDS             complement(4560..5828)
                     /locus_tag="AC16QF_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447151.1"
                     /translation="MKGATLLFAVLGITFTCVANAQTIEQIEEYERRIDEQQRQLDAL
                     RAELDVLKKVAGMPVSPDEEEATLEVAETLPDSAPESKPFLERRSDNGTFTLGGRLHR
                     VLMQVDDGASSNGFFMDSNQGPTMLRADVFAPLNGGWRLSGALEVGIQSNRPDRVTQD
                     NPSPGTDITVREADVELHNDKVGTFSFGRGFAAAWVVPEIDLSGTVPSSLLAVGNLAP
                     GMRFVDRTNDELSDISVNQHFADTERLLLVDRLRYDSPRLGGGFQLSGTVAADSRWDA
                     ALRYYPSNDDWTIRAAATYQHKPFQDLDYRVELGLSARHNRTGLSLTTGLLFGDASGR
                     DASGYIVKAGWLTNLTSLGSTAFSADFSSGSDIRLPDDDSESVGFFVQQKLDAVGLDV
                     YGGYRRYKVDRPDFDLRPLKVVVLGAMYTF"
     gene            complement(5934..7343)
                     /locus_tag="AC16QF_00985"
     CDS             complement(5934..7343)
                     /locus_tag="AC16QF_00985"
                     /inference="COORDINATES: protein motif:HMM:NF019157.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HIR6447152.1"
                     /translation="MAPHCKVVGVIGTETNFELLLPENWNGKFVMGGGGGFVGSVQNT
                     SLLLGSLQKGYATVGTDAGHAGHPLNASWAYNNLERLVSFGHQAVHRTAVTAKALITD
                     FYDEGISRSYFTGCSRGGGQGFMEAQRYPEDFDGIVAGSPAYDFPGVAAAATQISAAM
                     YPDPSNLQEAVVGPQEQRLIESSYLAMCDAMDGLEDGILNDPRQCEFDVATLLCEGEK
                     TDSCLSEEQLAAVRVVYDGPKDSQGRSLYYGFPFGGEGAEGGWSRWLTGGLKYDDDAD
                     DFQSGIDMGPFESPVTPSAYFSFGKEIMRYLIYNDPEWSYVGYDYDNLAKDGARAAET
                     LNATDPDLSAFRERGGKMLIYNGWSDNAQTSLAFADYYEQVLAHDESAADDVRMFLMP
                     GVEHCIGGPGPSWVNFLDEIDAWVESGKAPDQVVAYWLDETMQPAGSRPVCAYPAVAQ
                     YDGQGDPRDASSFSCSGGG"
     gene            7704..8735
                     /locus_tag="AC16QF_00990"
     CDS             7704..8735
                     /locus_tag="AC16QF_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6447153.1"
                     /translation="MSETSRHCSKPQVPIIRAANLMGFIDFLEKTAGPADGCLGRAGL
                     SRELTKRPECLISFPRAIRFLELASAQIGTPHMGWYVGRDHPISALGSYGQRLDACVT
                     VHDYLRTGVSLYPRVSNRDSLKLEEVREGLRIHHDSGLPPSLGTHQSDLHFIEITLGK
                     LREALGPSWTPGRVGIAHSPSEPVPADSLLASAGVLTKSGTAFIEVSKAELRAVFPRR
                     RRPTNSRPPSVSLSSLPADFVGLARAQIEMLLPTGRIGIELLAESMGLSVRSVQRRIA
                     SAESSFSELLEDARFRLATSWLEHTSKSVTEIAFDLGYQYPANFTRAFRARTGLPPNA
                     YRATFSGSA"
     gene            8870..10387
                     /locus_tag="AC16QF_00995"
     CDS             8870..10387
                     /locus_tag="AC16QF_00995"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008030174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004065 - arylsulfatase activity [Evidence
                     IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HIR6447154.1"
                     /translation="MITRIAATTAALLLSFAAWGAEQPNILVIWGDDIGITNISAYSD
                     GIMGYRTPNIDRIASEGVRFTDYYGDQSCTAGRSTFITGQSGLRTGLTKVGLPGAELG
                     IQDRDVTLAEVLRDMGYATGQFGKNHLGDKDEHLPTNHGFDEFLGNLYHLNAEEEPED
                     PDYPKVPGFRERFGPRGVIHSFADGKVEDTGPLTRKRMETADEEFVAAAKDFVDRSVK
                     ADKPFFVWLNTTGMHFRTHPAEKHLGKSGQGPYNDVMMAHDELVGQMLNQLDALGIAE
                     NTIVFYSTDNGVHFNTWPDAGVTPFRSEKNTNWEGAYRVPAMVRWPGKIAPGQVSNEI
                     MSHLDWMPTLVAAAGDVGLKDKLLEGTRIGRKQSKLHLDGYNFLPYLTGQAENGPRRE
                     FHYLNDEGMPVGVRVGDWKIVFAENRGKTMALWAEPFVTLRMFKIYNLRRDPFERAEH
                     NSNSYWDWMIDKAPQGYLGLAATAQFLQSFQAYPPSQRPDSWSIDRLTEALLEQQ"
     gene            10384..11976
                     /locus_tag="AC16QF_01000"
     CDS             10384..11976
                     /locus_tag="AC16QF_01000"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6447155.1"
                     /translation="MNVRSAIAGGLLCICWISGAAWGEAEPIAQADLPPDAAPADPYG
                     RDTPRGSMEGFLESAGEGDFERAARYLDLRNLPNALNRYSPETLARGLNLVLERGTWL
                     DLTRLSNSPEGDLGDGLPTYRDPLDTLSTSRGDAELWLQHVPSDVHEGMLIWKVSNRS
                     VARLPELYDEFRFSPPLEYLRTTLPDVRLLGVQLFKWVWSLAVGLAVWVILFGTSRLT
                     TRGAIRRGTRWGAPLRYFLSVPVSISASLLLAGAVLRSLGLGLEAQRISEMQTGNLIL
                     IAWVFFTGINFARDLYIGKLQSQGREGTGALIRPLAAALKSVLGIIFVITWLDNAGYN
                     IGALLAGLGIGGVAVALVLQKPLEDVFAAFTLFTQQPIRIGDFGRFGELTGTIEEISL
                     RTTRIRTLENTVVAVPNAKFAAEPIENISARRRILYRPYVRLEFGTTQEQVENILQGV
                     GKLLAGHPNVLEEGRRVRLLRFGEIGIEIEGFAYIDTTVYVDYLEIAEELNLAIMNIV
                     ESAGARFAQLSGDSVKQMVATG"
     gene            12108..13085
                     /locus_tag="AC16QF_01005"
     CDS             12108..13085
                     /locus_tag="AC16QF_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6447156.1"
                     /translation="MPYEQIQALNERMQRSIIGQEAVVERLIIGLLANGNLLVEGLPG
                     LAKTRAIKSLAKNLEADFSRIQFTPDLLPSDVTGTEVYYQQEGRSEFRFEPGPIFANI
                     VLGDEINRAPAKVQAAMLEAMEERQVTVAGTTHPMPELFMVMATQNPVEQEGTYPLPE
                     AQMDRFLMHVNIEYPPVEDEVLIMDLVRAEEGATGSDGGSGSEEARIPQQAIFDARAE
                     IAAIHLSEAIKRYMADLVYATRTPQRYSDDLDRWIDVGASPRGSLALDKCGRAHAWLN
                     RRDFVDPGDVRAIVHDCLRHRLMLSYEAKGEGVTSDKVIDVLLEKVALP"
     gene            13093..>13398
                     /locus_tag="AC16QF_01010"
     CDS             13093..>13398
                     /locus_tag="AC16QF_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016974755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6447157.1"
                     /translation="MSVDPRIHVTLQHLTSMQGAAGGITFLPRQASASVLNGRHASRL
                     RGRGLNFEEMRGYLPGDDVRSIDWKATARTGQPHVRVFTEERDRPALLVVDQRMSMFF
                     "
BASE COUNT         2535 a         4491 c         4131 g         2241 t
ORIGIN      
        1 cgatctacga ggtcacccag atcaaggggg acggtgaggc gcacccgctg ctctcgccca
       61 acgacgagtt cgccgattac gagacctggg accagggcaa cctgggcccc aatctcaagg
      121 acgacgacat gctgcagtac gagtatgccc gggaggcgct caagaagggg ctggctcatg
      181 aagtccggct cggcgtcaac ccctacaagt tcggcctgca gggggcgacg gactcccaca
      241 ccggcctcgc caccgccgag gaggacaact tcttcggcaa gcactcgcag accgagccca
      301 ccgcggcgag actggccacc gacgtcggcc atttcgagaa caacgtctgg cccggctggt
      361 atcaggtggc gtcgggctac acgggagtgt gggccacgga gaacacccgg gaggcgatat
      421 tcgacgccat gatgcgcaag gaggtctacg ccgccaagta tttcggcacc acagctcccg
      481 aggaatcccc catgaccacc caggaacgtg catatacctc accgatctgg tacaccccct
      541 gaccgggaaa gtcactcgaa ggaagcggac catgaaaaca aggatgattg ggggcacttg
      601 cttggccggc attctcactc tgctggccgc ctgcgacccg gccggtaccg agcccacggg
      661 cacggacacc gacgccgcgc cccaacccgt caccgtgggc ccggccccgt cggcaatggg
      721 agacagaagg ctactctggg gggacaccca cctgcacacg tcctactcac cggacgcgta
      781 cctgatgcag aaccgctcgg cggatccgga caccgcctac cgctacgcca aggggctgcc
      841 cgtggtgcac ccctatcaca aggcgcgcat ccagatcggc acgccgctgg attttctggt
      901 ggtttccgat cacggcgagt tcatgggcgt ggtgcccatg gtgctccagg gtgatccccg
      961 ggtcgccaac acggagaccg gcaagcgcta tcgggcaatg gccgaggcgg gccgcgagat
     1021 cgaggtgttc ggcgagctca tcgcccaggt caacaagatc gtcccaccgg accaggacct
     1081 ctacaacccg gacatcaacc gctcggtttg gggcgagatc atggaagccg ccgaccgtca
     1141 caacgagccc ggcaccttca ccgcgttcat gggctgggag tggtcgtcca cgccccaggg
     1201 gcagaacctg caccgggtga tcgtcatgcg cggcgacaag gacaacggcg acgagttcgt
     1261 tccctatacc agcctggaca gcgaccggcc cgaggatctg tgggcgtggc tggaggagac
     1321 tgccgaggcg gccggcaccg actttctcgc catcccccac aactcgaaca tctccggtgg
     1381 gctcatgttc ccgctggtga acagcgacaa agaagccgtg gacgcggagt acgcgaagct
     1441 gcgcatgcgc tgggagccgg tggtggagat gacccagatc aagggcgact ccgagaccca
     1501 tcccatgctc tcaccgaatg acgagttcgc cgactacgag acctacgagc acctcatcgc
     1561 tgccgaaggc atcgagcagc agaccatgtt caccgagggc tttctcggca cgttgagcga
     1621 cgcggaccga gccgtgctcg aaagccagga gaagcgcatc gtcaagaatg gcgactacgc
     1681 ccgaacgggg ctgctccgcg ggctggtgct cgaagagcgc gggcgcggca acccctacaa
     1741 gttcggcatg attggctcga cggactctca taccgccatg gcatccgccg aagaggacaa
     1801 cttccacggc aagatggcca ttgactccac gccgggaacc aagcgcgaag acatcattcc
     1861 cggcacgccg ggctgggaca tgggcgcggc gggcttggca gcggtgtggg ccacggagaa
     1921 cacccgcgag gggatcttcg acgccatgaa gcgcaaggag gtctacgcca ccacggggcc
     1981 gcgcatcgag ctgcgcttct tcgggggttt cgacttcgcc gccgctgacg cccaggcccg
     2041 ggatctggcc tcggtggggt acgggaaagg ggtccccatg ggcggtgatc tgaccgcgtc
     2101 acccgcgggc aacgcgcctt cgtttctggt gagcgccgtg aaggatccag tgggcgccaa
     2161 tctcgatcgg gtccagatcg tcaagggctg ggtggacgcc gaggggatgc cccaggagaa
     2221 gatcttcgac gtggcgctgg cagacggccg aacggtgggc acagatggga aagtcgcccc
     2281 tgtgggcaat acggtggacg tctccaccgg ccattacacc aacgccatcg gggatcccca
     2341 gctcaccgtg gtctggaggg acccggagtt cgaccccggc aagcgcgcct tctactacgt
     2401 gcgggtgctg cagattccca ctccccgaca cacgctctac gacgccatcg cgctcgggat
     2461 atcggtggaa gagacggggc atccggcgac cattcaggaa cgggcgtact cctcgcccat
     2521 ctggtacgca ccctgacgac gtttggactc tgaccccccg ctgccgccca cccggcggca
     2581 gcgagcggcg tcattttaga gagagccatg aacaagcttc tcaaagaacc ccttctgcac
     2641 ttcctcgttc tggggctggc cctgttcgtg ctgtttgacg tggtctccga cgagaaggaa
     2701 acgtcagaca gcaagctcat cagggtggat cgcgagaccc tgctgacctt cattcagttc
     2761 cggacccgcg ccttcgagcc gagcatcgct gcccagcggc tcgacggcct ttccgatcag
     2821 gagctcaagc tgctcatcga cgactacatc cgcgaggagg ccctgtaccg cgaagcgctc
     2881 gccctgggca tggacaagaa cgactacgtg atcaagcgcc gcatgatcca gtccatcgag
     2941 ttcatcacca ggggcttcgt cacccgggcc gtggaggtgt ccgacgacga cgtgaaggcc
     3001 cattacgagg ccaatcggaa cgattactac gtggaaccgt ttgtcacgtt cacgcacgtc
     3061 ttcatatccg gtgagaaccg cagcgaagcc gaggtcgacg cgctggctgc cgccgagctc
     3121 accgagctca atgcccgcca gataccgttc agcgccgccc ccgggcgagg ggagcgtttc
     3181 ccctttttcg tcaactacgt ggagcgcgac ccggaattcg tcgccagcca cttcggggct
     3241 gacatggcga acgagatctt cgccctggaa cccgacgagc tcgtctggcg tggaccattt
     3301 ctgtcaacct acggcgccca cctggtcctg ctgacccgca aagcgcccgg ccgctttccc
     3361 ccgctcggtg agatcgaaga aagcgttcgg caggacgccg agcgagccgc gttgcagaag
     3421 gcgaacgaaa ccgccattca ggccatcgtg gacacctacg aggtggagcg gcagcccgag
     3481 ccccgcacgc gcgccggcgc aggatgagaa ctctccgctc agccgtcctg ggtctgtggg
     3541 cgtgctgcgc tgcgctgccc gccgtcggcc acgatgcgcg gccgaactac gtccagatca
     3601 cggagacggc gcccaatagc ttcaacgtgg cgtggaaggt tcctgccagc atgccgggca
     3661 acgcgctgcc ccatcctctg ctgccggacg actgcaagcc gcagcagcct cccaggtggc
     3721 acaccaccgg ggcagagcac gcggcgcaac aggaattcat ctgcgagcag ggcctctccg
     3781 gacgggtcgt gggcatcgag tttcccgtca tcaacccttc cctctccacg ctgtaccgcg
     3841 ttcggctggc aaacggcgaa gagcatctga agatcctgaa gcccgacgag acggtgtgga
     3901 tggttcccga tgcggaggac cgtctctcgg tcgcgaagga gtacacgggg ctcgggatcg
     3961 agcacatctg gatcggcctc gaccacctcc tgttcgtcgc ctgcctgctg ttcatctcgc
     4021 gcacgccccg acgcctgctc atcaccatca cgggcttcac cgtggcgcac tccatcaccc
     4081 tggcgctgtc agctctcgac ctggtgcgca tccccacgcc gccggtggaa gccgtcatcg
     4141 cgctcagcgt ggtgttcatg gcctgggaga ttgtgcgcgg cgacgacgac tctttggcct
     4201 accgttttcc ggtcgccgtt tccagctcct tcggtctcct ccacggcttc ggtttcgccg
     4261 ccgtgctgcg cgacattggc ctgccccaga cggagctgcc caccgcgctg ctgttcttca
     4321 acgtgggcgt ggagatcggc cagatactct tcgtgatggc gctgctgatc gccttcgtag
     4381 cgctgcggcc ggcccttggg cgtgcgctgc gcgctgagcc cggcggagcc gttcactggt
     4441 cgtacctgac caccccggca agctacgtca tcggcaccct ggcgagctac tggctgatcg
     4501 agcgccttgc tggcttctgg gggtgatcgc gcaccccgcg gattgatcgc tcgcccctgt
     4561 cagaacgtgt acatcgcccc cagcacgacg accttcagcg gccgcagatc gaaatcaggc
     4621 cggtccacct tatagcggcg ataacctccg tagacgtcca gcccaacggc gtccagcttc
     4681 tgctggacga aaaagccaac ggattccgaa tcgtcgtcag ggaggcgaat atcgctgccc
     4741 gatgaaaagt cggcggaaaa cgccgtcgat ccgagactcg tgaggttggt gagccagccc
     4801 gccttgacga tgtaaccgga cgcatcccgc cccgaagcgt cgccgaacaa caggcccgtg
     4861 gtcaggctca gacccgtccg gttgtgccgc gcggaaaggc ccaattcaac tcgatagtcg
     4921 aggtcttgga acggtttgtg ctgataggta gcagccgcgc gtatggtcca gtcgtcgttc
     4981 gacgggtagt agcggagcgc cgcatcccag cgcgagtcgg cggcgacggt ccccgacagc
     5041 tgaaagccgc caccaaggcg gggcgagtca taacgcagac ggtcgacgag cagcagccgc
     5101 tccgtatcgg caaagtgctg attgacgctg atgtcgctca gctcgtcgtt cgtccggtcg
     5161 acgaaccgca tgccgggtgc aaggttgccg acggcaagca gcgaggacgg gaccgtgccg
     5221 gaaagatcga tctcgggaac cacccaggca gccgcgaagc cgcgcccgaa actgaacgtg
     5281 cccactttgt cgttgtgcag ctccacgtcc gcttctctga cggtaatgtc cgtacccgga
     5341 ctcggattgt cctgggtcac gcggtcgggc cggttgctct ggattcccac ctcgagagcg
     5401 ccggacaacc gccatccgcc gttcagcggc gcaaacacgt ccgcccgcag catggtcgga
     5461 ccctgattcg aatccatgaa aaacccgttg ctcgatgcgc cgtcatcgac ctgcatgaga
     5521 acccgatgca ggcgcccgcc caaggtgaac gtaccgttgt cgctgcggcg ctcgaggaac
     5581 ggcttcgatt ccggcgcgga atccggcagc gtttcggcca cctccagcgt tgcctcctcc
     5641 tcatccggcg aaaccggcat gccagcgact tttttgagta cgtcgagctc ggcgcgcagg
     5701 gcgtcgagct gccgctgctg ctcgtcgatg cgccgctcgt actcctcaat ctgctcaatc
     5761 gtttgcgcgt tcgcgacaca ggtaaacgtg atccccagca ccgcgaacaa aagtgtcgcg
     5821 cccttcatga tgattcccct caggctgccc aggcgatgaa gcaagctcgg cgcaatggtc
     5881 aacaccccat tgcgccgacg agttccatat gacctgattt tgtcggctat ttgtcagccg
     5941 cccccggaac agctgaaact cgacgcatcc cgcggatcac cctgaccgtc gtactgcgcg
     6001 actgcagggt aggcacagac aggcctcgat ccggccggct gcatggtctc gtcgagccag
     6061 taggcgacga cctggtccgg ggccttgcct gactcgaccc aggcgtcaat ctcatcgagg
     6121 aaattgaccc aggagggccc tggtccgccg atgcaatgct ccacgcccgg catcaggaac
     6181 atacgcacgt catccgcagc cgactcatcg tgcgcaagga cctgctcata gtagtcggca
     6241 aacgcgagag atgtctgcgc attgtccgac cagccgttgt aaatcagcat tttgccgccg
     6301 cgctcgcgaa acgccgacag gtccggatcg gtggcattga gcgtctcggc agcacgggcc
     6361 ccgtccttgg caaggttgtc atagtcgtaa ccgacgtaac tccactcagg atcgttgtag
     6421 atcaaatacc gcatgatctc cttgccaaat gagaaatagg cactcggcgt cacgggcgat
     6481 tcaaatggcc ccatgtctat accggactga aagtcatcgg cgtcgtcgtc gtacttcagc
     6541 ccgccggtga gccagcggga ccaccccccc tcggcgcctt caccaccgaa cggaaatccg
     6601 tagtacaagg accgtccctg ggaatccttg ggcccgtcgt aaacgaccct gaccgccgca
     6661 agctgctcct cgctgaggca gctgtcggtc ttctcccctt cgcacagcaa agttgccaca
     6721 tcgaactcgc actgtcgggg gtcgttgagg atcccgtctt ccaaaccgtc catcgcatcg
     6781 cacatggcga ggtacgacga ctcgatcaaa cgctgctcct gcgggcccac cacagcttcc
     6841 tgcaggttgg aaggatcggg gtacattgcc gcgctgatct gggtggccgc cgcagcaacc
     6901 cccggaaaat cgtatgccgg tgagccggcc acgatgccgt cgaaatcctc cggataccgc
     6961 tgagcctcca tgaagccctg cccccctccg cgagagcatc cggtgaagta gctgcggctg
     7021 atgccctcgt cgtaaaagtc ggtgatcagc gccttcgccg ttaccgcggt gcggtggacg
     7081 gcctggtggc caaagctgac gaggcgctcc aggttgttgt aggcccagct cgcattgagc
     7141 ggatgccccg catggccggc gtcggtgccg accgttgcat agcctttttg cagggatccg
     7201 agcaaaagcg aggtgttttg cacgcttccg acgaacccac cgccgccgcc catgacgaac
     7261 ttgccgttcc agttctccgg cagcagaagc tcgaaattgg tctcggtacc gatcacgccg
     7321 accaccttgc aatgcggcgc catcgaggcc tcctgcgtga ccgagatgag ccggacgtcg
     7381 ggaagtgccg gaaacgatgc ctgagaacag accggacccg aggtcgccgg caggctctgc
     7441 tccgaggttc cgcacgccgt gagcacggct gtcagcagaa ttaacaagcc tacatctaat
     7501 ggcctttcaa acatcatttc gtccccttcc actctcgatc gcaatcagcc agatcctcag
     7561 gtaaggcaac agaaacctca acccccgagg cagtttccga ccggcactgc cgatccgacg
     7621 atgttcacaa gcatattcac ctgctccgcc attcgcttat catttgacgc caccctcgca
     7681 aaggcgctga gcaagaggga accatgagcg agaccagcag gcactgcagc aagccacagg
     7741 tgccaattat ccgtgcggcc aacctgatgg gcttcatcga ctttctggag aaaaccgccg
     7801 ggccagcgga cggctgcctg ggacgtgcgg gtctttcccg ggagctcacg aagcgtcccg
     7861 aatgtctgat ttctttccca agggcgatca gattcctgga gctggcatcg gcccaaatcg
     7921 gtaccccgca catgggttgg tacgtcgggc gggatcatcc catcagcgcg ctaggcagct
     7981 atggccagcg gctcgacgcc tgcgtgaccg tgcacgacta cctgcgcacc ggggtgtcac
     8041 tttacccgag agtaagcaac cgggactccc tcaagctcga agaggtccgc gagggcctgc
     8101 ggatacatca cgactccggc ctcccgccaa gtttaggaac ccatcagagc gatcttcact
     8161 tcatcgagat tactctcggc aagctccggg aagccctcgg accttcctgg accccaggcc
     8221 gtgtgggcat cgcgcacagc cccagcgaac ccgtgcccgc ggattcgctg ctcgcaagcg
     8281 ccggtgtcct taccaaaagc ggaacggcat tcatcgaagt cagcaaggcc gagctacgcg
     8341 ccgttttccc gcgcagacgc agaccaacca acagccgccc gcccagcgtc agcctctcga
     8401 gccttcccgc ggacttcgtc gggctggccc gagcgcagat cgaaatgctg ctgccgaccg
     8461 gacgcatcgg catcgagctg ctggcggaga gcatggggtt gtcggttcgc agcgtgcagc
     8521 gtcgcatcgc aagcgcggaa tcgagtttct ccgagctgct ggaggacgct cgatttcgac
     8581 tcgcaactag ctggctggag cacacttcca aatcggtcac cgagatcgcc ttcgacctgg
     8641 gatatcaata ccctgccaac ttcactcgag cgttccgagc gagaacgggg ttgccgccga
     8701 atgcctatcg agcaaccttc tccggaagcg cctgactttc gaagcggcat cgcccgacct
     8761 gacccccgcg cttcatcgcc ccctcgaatg gaggtagcat cgcaaacttc gttgcgggca
     8821 aagcgatttc cgcatcacgc tcgaagcggc acgcgcagag gaaaacatga tgatcacacg
     8881 gatagcggcg accaccgccg cattgctgct gagcttcgcc gcctggggcg ccgagcagcc
     8941 gaacatcctc gtgatctggg gcgacgacat cggcatcacc aacatcagcg cgtacagcga
     9001 cggcatcatg gggtaccgca cgccgaacat cgaccgtatc gccagcgagg gcgtgcgctt
     9061 caccgactac tacggcgatc agagctgcac ggcaggccgg tccactttca tcactggcca
     9121 gtcgggcctg cgaacggggc tgaccaaggt gggcctgccc ggcgccgagc tgggcattca
     9181 ggaccgagac gtgacgctgg ccgaggtgct cagggacatg ggctacgcca ccgggcagtt
     9241 cggcaagaac cacctgggcg acaaagatga gcatctgccc accaatcacg gctttgacga
     9301 gttcctgggc aacctctatc acctgaacgc cgaggaggag cctgaggatc ccgactatcc
     9361 aaaggtcccc ggcttccggg aacggttcgg gccgcgcggc gtgatccact cgttcgccga
     9421 tgggaaggta gaggataccg ggccgctcac gcgcaagcgc atggaaacgg cggacgagga
     9481 attcgttgcc gccgcgaagg atttcgtcga ccggtccgtc aaggccgaca agccgttctt
     9541 cgtctggctc aacaccacgg gcatgcactt ccgcacccac ccggcggaga agcacctggg
     9601 caagtcgggc cagggcccgt acaacgacgt catgatggcc cacgacgagc ttgtcgggca
     9661 gatgctgaac cagctggacg cgctgggcat cgccgagaac accatcgtct tctactccac
     9721 ggacaacggc gtgcatttca acacctggcc ggacgccggg gtcaccccgt tccgaagcga
     9781 aaagaacacc aactgggaag gcgcctaccg cgtgccggca atggtgcgct ggcccggcaa
     9841 gatcgctccc ggtcaggtct ccaacgagat catgtcccac ctggattgga tgccgacgct
     9901 cgtggcagca gccggggatg tcgggctcaa ggacaagctt ctcgagggca cgcggatcgg
     9961 ccgtaagcag tcgaagctgc atctggacgg ctataacttt ctgccctatc tgaccggcca
    10021 ggccgaaaac gggccgcgcc gggagttcca ctacttaaac gacgagggca tgcccgtcgg
    10081 cgtgcgcgtc ggcgactgga agatcgtctt cgccgagaac cgcggcaaga cgatggcgct
    10141 gtgggcagag ccgttcgtca cgctcagaat gttcaagatc tacaacctgc gccgtgatcc
    10201 cttcgagcgt gccgagcaca actcgaactc ctactgggac tggatgatcg acaaggcgcc
    10261 tcagggctac ctgggcctcg ccgccacggc gcagttcctg cagtcctttc aggcgtaccc
    10321 gccgagccag cggccggaca gctggtccat cgaccggctg accgaagcgc ttctcgagca
    10381 gcaatgaacg tccgcagcgc catcgccggg ggcctcctgt gcatctgctg gatcagcggc
    10441 gcggcctggg gagaagcgga accgatcgcc caggccgatc tgcccccgga cgccgcccct
    10501 gccgacccgt acgggcgcga caccccgagg ggatcgatgg aaggcttcct ggaatccgcc
    10561 ggcgagggcg acttcgagcg tgcggccaga tatctggatc tgcgaaacct gccgaatgct
    10621 ctgaatcggt actcaccgga aacgctcgcc cgagggctca atctggtgct ggagcgggga
    10681 acctggctgg acctgacccg gctcagcaac agcccggaag gcgacctcgg cgacggcctg
    10741 ccaacctatc gggatcccct ggacaccctg tcgaccagtc gcggcgacgc cgagctttgg
    10801 ctgcagcacg tcccgtcgga tgtgcacgag ggcatgctga tctggaaggt atcgaatcgc
    10861 agcgtggcca ggctgccgga gctctacgac gagttccgct tctctccgcc cctggagtac
    10921 ctgcgcacca cgctgcccga cgtgcgcctg ctgggcgtgc agctgttcaa gtgggtctgg
    10981 agcctggcgg tggggctggc cgtctgggtg atcctgttcg gcacgtcgcg cctgacgaca
    11041 cggggggcca tccggcgagg gacacgttgg ggagcgcctt tgcgctactt cctgtcggtc
    11101 cccgtctcca tatccgcaag cctgctcctg gccggggccg tgctccgctc tctcgggctg
    11161 gggctcgagg cccagcgcat ctcggaaatg cagaccggca atctcatcct gattgcctgg
    11221 gtcttcttca ccggcatcaa cttcgctcgg gacctgtaca tcggcaagct gcagagccag
    11281 ggccgcgagg gcacgggcgc gctgatccgg ccgctggccg ccgcactcaa atcggtgctg
    11341 gggatcatct tcgtcatcac ctggctcgac aacgccggct acaacatcgg cgccctcctg
    11401 gccggcctcg gcatcggcgg cgtagccgta gcactcgtgc ttcagaaacc cctggaggac
    11461 gtgttcgcag ccttcacgct gttcacccag caacccatcc ggatcggcga cttcggccgc
    11521 ttcggagagc tcaccggcac catcgaggag atcagcctgc gaaccacgcg gataaggaca
    11581 ctggagaaca ccgtggtcgc cgtgcccaat gcgaagttcg ccgcggagcc catcgagaac
    11641 atctcggctc gccgccggat cctgtaccgg ccttacgtcc ggctggagtt cggtaccacc
    11701 caggagcagg tggagaacat cctgcaggga gtcgggaagt tgctcgcggg tcatccgaac
    11761 gtgctcgagg agggccgccg ggtaaggctg ctgcgatttg gcgagatcgg catcgagatc
    11821 gaaggctttg cctacatcga caccaccgtg tacgtggact atctggagat cgccgaggag
    11881 ttgaacctcg ccatcatgaa catcgtggag agcgcgggcg cccgattcgc tcagctctcc
    11941 ggcgacagcg tcaagcagat ggtggcgacc ggctgatccg tgtgcagatg ggggtcgcag
    12001 ccgcgctgtc gacacgtccg cgccgccgtc ggcatactgc tgcctgacac caccgggatc
    12061 attcgtcgcg ccgctgaccc ggacgcggcc gggaagcacg acacaccatg ccctacgaac
    12121 agattcaagc gctcaacgag cgcatgcagc gctccatcat cggccaggaa gccgtggtgg
    12181 agcggctcat catcggcctg ctcgccaacg gcaacctgct cgtcgagggc cttcccggcc
    12241 tcgcgaagac ccgggcgatc aagtccctgg ccaagaacct ggaggccgac ttcagccgca
    12301 tccagttcac cccggacctg ctgccctcgg acgtgacggg cacggaggtc tactaccagc
    12361 aggaggggcg cagcgagttc cggttcgagc ccggcccgat tttcgccaac atcgtcctgg
    12421 gcgacgagat caaccgggcg cccgcaaagg tccaggccgc catgctggaa gcgatggagg
    12481 aacgccaggt caccgtggcg gggacgaccc atcccatgcc cgagctcttc atggtcatgg
    12541 ccacccagaa cccggtggag caggaaggaa cctacccgct gccagaggcg cagatggacc
    12601 gctttctgat gcacgtcaac atcgagtacc cgccggtgga ggacgaggtg ctcatcatgg
    12661 atctggttcg ggcggaagaa ggcgccaccg gcagcgatgg cggctccgga tcggaggagg
    12721 cgcgcatccc ccagcaggcg atcttcgacg cccgggcgga gatcgcggcg atccacctct
    12781 ccgaagccat caagcggtac atggcggatc tggtctacgc cacccgcacc ccgcagcgct
    12841 actcggacga cctcgaccgc tggatcgacg tcggtgcgag cccccggggg tccctggcgc
    12901 tggacaagtg cgggcgcgcc cacgcgtggc tgaatcgtcg ggatttcgtc gatcccggcg
    12961 acgtccgagc gatcgttcac gactgcctgc gccaccggct gatgctctcc tacgaagcca
    13021 agggcgaggg cgtcacctca gacaaggtga tcgacgtgct gctggagaag gtggccctgc
    13081 cctgactcga gcatgagcgt cgatccccgc atccatgtca ccctgcagca cctcacctcc
    13141 atgcagggag cggccggcgg catcacgttc ctgccgcgac aggcttcggc cagcgtcctc
    13201 aacggccggc acgcttcccg tctgcggggc cgcggcctga acttcgagga gatgcgcggc
    13261 taccttcccg gcgacgatgt ccgctccatc gactggaagg ccaccgcgcg caccggccag
    13321 ccccatgttc gggtctttac cgaggagcgt gatcgacccg cgctgctggt cgtggatcag
    13381 cggatgtcca tgttcttc
//
LOCUS       DBNHLM010000019         7059 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1498574, whole genome shotgun sequence.
ACCESSION   DBNHLM010000019 DBNHLM010000000
VERSION     DBNHLM010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7059
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1498574"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="AC16QF_01015"
     CDS             <1..345
                     /locus_tag="AC16QF_01015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6447158.1"
                     /translation="VRMLPHNLFKSWWKIRQWAPYDLVIVDPPTNQGGSFNAEKQYGQ
                     ILKRLRELTRPGGRVLACLNSPFLNRDFLPAQMSRWCPECQFEGYLANPDDFPDRYPD
                     RALKVGVFTRSG"
     gene            complement(337..798)
                     /locus_tag="AC16QF_01020"
     CDS             complement(337..798)
                     /locus_tag="AC16QF_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447159.1"
                     /translation="MSDINIDDFFKDAAIALVQLYGMFPRRHALFVEDVCGPDETDEF
                     GMHSDRYLACFGTLLWLGEEGYVRYEQSIRQDAIDQAVLTARCFTVLATPAPRPTPPD
                     TDCNLPESIRVEQSTTINRLRAALKARSSPQLRAEMVELMTQMGTRLNVSS"
     gene            complement(795..2081)
                     /locus_tag="AC16QF_01025"
     CDS             complement(795..2081)
                     /locus_tag="AC16QF_01025"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR6447160.1"
                     /translation="MDSAENDKVTPLPASAAVVTGEAGRRQILKSHKLDDVCYDIRGP
                     VLEEAHRLEEEGHRVLKLNIGNPAPFGFEAPEEILLDVIHNLPESQGYSDSKGIYSAR
                     KAIMQYCQQQGVPNVEIDDIFLGNGVSELVVMSLQGLLNNGDEVLIPAPDYPLWTAAT
                     HLSGGRAVHYRCDEQSDWNPDLADIKARITDRTKAIVIVNPNNPTGAVYDRSLLVELV
                     ELARANDLVIFADEIYDKILYDDAEHVPLATLAEDLLVVTMNGLSKTYRVAGFRSGWM
                     VVSGAKHRAQDYMEGLNILASMRLCANVPAQHAIQTALGGYQSIQQYLVPGGRLYEQR
                     SVAWEMLNAIDGVSCVKPKGALYLFPKLDVRKFNITSDERFVLDLLRQERILVVQGTG
                     FNWFEPDHFRIVFLPHEEDLRHALERLEHFLAGYRQ"
     gene            2192..2599
                     /gene="msrB"
                     /locus_tag="AC16QF_01030"
     CDS             2192..2599
                     /gene="msrB"
                     /locus_tag="AC16QF_01030"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR6447161.1"
                     /translation="MGKVTKSDDEWRSELDTHTYEIARKAGTERAFTGKYWDTKTPGI
                     YHCTCCGASLFDSDTKYDSGSGWPSFYEPVSHDAVSTREDNSLMMQRIEVLCAECDAH
                     LGHLFPDGPEPTGLRFCMNSASLDLQERDEDGS"
     gene            complement(2605..4116)
                     /locus_tag="AC16QF_01035"
     CDS             complement(2605..4116)
                     /locus_tag="AC16QF_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR6447162.1"
                     /translation="MASSQPLAVQAGLDAMRSGGNAVDAALATAIALTVVEPNNNGVG
                     SDAFAIVWDGSKLVGLNASGRSPAGWTPDRFAGQRTMPELGWDAVTVPGAVSAWSALS
                     ERYGRLAFPDLFDAAIRYAAEGFPVGPKTAYYWQFAERRYADFPDFANHFLPAPRAGE
                     RFRRPDLARTLQEIAATGGKAFYEGDIAERIAAAAATDGGVMTRDDLAAHRAEWVDPL
                     SQPYRSVDVHEIPPNGQGLAAQIALGIFAHFDAPPLETAESVHLQVEAMKIAIRAAAD
                     HFADADAMRLPPAELLDPANLRRAAGTIGRSASTLPPVSLPTSQDTVYLCAADASGMM
                     VSFIQSNFMGFGSGIVIPGTGISMQNRGSGFTLEPGHPNRVGPRKRPYHTIIPGFVTE
                     NGRPRLAFGVMGGHMQHQGHFQMVQRIFDHGQNPQAASDAPRWRVFPDYSLRVEPEFP
                     EKVIKELADRGHQIGVEDQEHQFGGAQLIYRNESEDAYIAGSDHRKEGMAAGF"
     gene            4259..5788
                     /locus_tag="AC16QF_01040"
     CDS             4259..5788
                     /locus_tag="AC16QF_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6447163.1"
                     /translation="MHPGIIAAANPDKVAYLMADSGETITYGELEKTSNQGAHLFRQL
                     GLKRGDHIALLLENHPRFFQICWAAQRAGLYYTAISWRLQQAEVEYIVNNCEAAVFIT
                     SAARREVVAPLADSMPGVSHRFVIDGAIEGFDDWAEAISAMPVTPVADESEGMAMLYS
                     SGTTGYPKGVKRPLPDGEYGAAAGINLLNILYGADDDSIYLSPAPLYHAAPLGFTMGC
                     LRYGIQVVVMEHFEAELALRYIQDYRITHSQWVPTMFVRMLKLPEEIRLRFDTSTLRC
                     AIHAAAPCPVPVKEQMIEWWGPVIYEYYAGTEGNGFVQLNATEWLEHKGSVGRPLNCE
                     IHICGDDGEELPAGESGTIYFGGGGEFEYHGDPQKTAGSRHPKGWSTLGDVGYLDEDG
                     YLYLTDRKHFMIISGGVNIYPQEAENVLITHDAVMDVAVFGVPNPEFGEEVKAVVQPT
                     DMALATPELAVELMDYCRARLSHIKCPRSVDFREELPRHPTGKLYKRLLKDEYWSKAG
                     G"
     gene            complement(5798..6688)
                     /locus_tag="AC16QF_01045"
     CDS             complement(5798..6688)
                     /locus_tag="AC16QF_01045"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004766 - spermidine synthase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HIR6447164.1"
                     /translation="MMRIAAGLFTFVLLLPAGDAQGRIVHRERSLYSTILIDQQGSLV
                     CLQFSIRTDQRNQSCMDQRRPNEMVFSYTRMMMTGLLLRPSPERILMIGLGGGTLPTA
                     LAELYPDTEIDVVEIDPAVVRVAGEFFGYVPSANTTVFTQDGRVFIKRAALRGDTYDL
                     IMLDAFNGDYIPEHLMTREFLQETRSLLTPGGAVVANTFSISQLYHHESTTYTAVFGR
                     FYNLKTGESANRIILAANGPLPDPTIMNQRAADLAPRVRKYGISLSDHVKGLETRPDW
                     DTSARVLTDQYAPANLLRDR"
     gene            complement(6685..>7059)
                     /locus_tag="AC16QF_01050"
     CDS             complement(6685..>7059)
                     /locus_tag="AC16QF_01050"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HIR6447165.1"
                     /translation="GAVTLYPLVYFAEPAMVWIFTRIEDPRYGSLLASSALFIVPTVI
                     LGMISPYSVRLLVASTHESGHVAGRLYFMSTLGSALGTLATSFYFVLWFEVNTILALM
                     TATLAAMGIIAVTAHRWGWQAS"
BASE COUNT         1282 a         2113 c         2356 g         1308 t
ORIGIN      
        1 gtgaggatgc tgcctcacaa cctgttcaag tcatggtgga agatccgtca gtgggcgcct
       61 tacgatctgg tgatcgtcga tccgccgacc aaccaggggg gcagcttcaa tgcggagaag
      121 cagtacggac agatcctcaa gcgtcttcgc gagctcacgc gcccgggcgg gcgcgtgctg
      181 gcctgtctca actcgccctt tctcaaccgc gattttcttc ccgcccagat gtcccggtgg
      241 tgtccggagt gccagttcga aggttatcta gccaaccctg acgacttccc cgaccgatac
      301 cctgatcgag cgttgaaggt aggcgtgttc actcgttcag gatgacacgt tcaggcgcgt
      361 gcccatctgc gtcatcagct ccaccatctc cgcacggagc tgcggcgatg agcgggcctt
      421 gagcgcggcg cgaagacgat tgatggtggt cgactgctcc acccggatgg actccggcag
      481 attgcaatcc gtatccggcg gtgtgggtcg gggtgcgggc gtggcgagga ccgtgaagca
      541 gcgggccgtc aggaccgcct ggtcgatggc gtcctggcgg atgctctgct cgtagcgaac
      601 gtagccctcc tcgccgagcc agagcagggt gccgaagcag gccagatacc ggtcgctgtg
      661 catgccgaac tcatcggttt cgtccggacc gcagacgtcc tcgacgaaca gggcatgacg
      721 ccgggggaac atgccgtaga gctgcaccag ggcgatggcg gcgtccttga aaaaatcatc
      781 gatgttgatg tcgctcactg ccgatagccg gcaaggaagt gctcgaggcg ctccagcgcg
      841 tggcgcaggt cctcctcatg gggaagaaac acgatgcgga aatggtcggg ctcgaaccag
      901 ttgaagccgg tgccctgcac gacgaggatc ctttcctgac gcagcaggtc gagcacaaag
      961 cgctcgtcac tggtgatgtt gaacttgcgg acgtccagct tcgggaacag gtaaagggcc
     1021 cccttgggct tgacgcagct caccccgtcg atggcattca acatctccca tgcgacgctg
     1081 cgctgctcgt aaaggcgtcc ccctgggacg aggtactgct ggatgctctg atagccgccc
     1141 agggcggtct ggatggcgtg ctgggcgggg acgttcgcac acaggcgcat ggatgccagg
     1201 atattcagcc cttccatgta gtcctgagcg cggtgcttgg caccgctgac gaccatccag
     1261 ccgctgcgga acccggcgac ccgataggtt ttcgacagac cgttcatggt caccacgagc
     1321 aggtcttctg ccagcgtggc cagcggaacg tgctcggcat cgtcgtagag gatcttgtcg
     1381 tagatctcgt cggcgaagat gaccagatcg ttggctcgag ccagctccac cagctcgacc
     1441 agaagacttc tgtcgtaaac ggctccggta gggttgttcg gattcacgat cacgatggcc
     1501 tttgtccggt cggtgatgcg tgccttgatg tctgccaggt ccgggttcca atcggactgc
     1561 tcgtcgcaac gatagtgcac ggcccgcccg ccgctgaggt gtgttgctgc ggtccacaac
     1621 gggtagtcgg gcgcggggat gagcacctcg tcgccgttgt tgagcaggcc ctgcagcgac
     1681 atgaccacga gctcggacac cccgttgccg agaaagatgt cgtcgatctc cacgttgggc
     1741 acgccctgct gctggcagta ctgcatgatg gccttgcgcg ccgagtagat gcctttcgag
     1801 tccgaatagc cctgggattc aggaaggttg tgaatgacgt cgagaagaat ttcctcgggc
     1861 gcctcgaagc cgaacggtgc cgggttgccg atattgagct tgaggacccg atggccttcc
     1921 tcctccagcc tgtgcgcttc ctccagcacg ggcccgcgaa tgtcgtagca gacgtcgtcg
     1981 agcttgtgcg acttgagaat ctgccggcgc cccgcttcac ccgtgacgac cgcggccgac
     2041 gccggtagcg gcgtgacttt gtcgttctct gcgctatcca tcgttgggga tccaggtttc
     2101 catcggctgc cttccaggca tagaatttcg cgcgctgcga tgttggcggc ggcgattgta
     2161 aactggtggc atggtagcag gggaaccaga tatgggtaag gtgaccaaat cagacgacga
     2221 gtggcgctcg gagctggaca cgcacacgta cgagatcgcc cggaaggccg gcaccgaacg
     2281 ggcgtttacc ggaaagtact gggataccaa gacgccgggt atctatcatt gcacctgctg
     2341 cggcgcctct ctgttcgatt ccgacaccaa gtacgactct ggctcgggtt ggcccagctt
     2401 ttacgaaccc gtctcccacg atgcggtgag cacgcgcgag gacaacagcc tgatgatgca
     2461 gcgcatcgag gtgctctgtg ccgaatgcga cgcccacctg ggtcacctat ttccggacgg
     2521 tcccgagccg accggattgc gcttctgcat gaactccgcc tcgctcgatc ttcaggaacg
     2581 cgacgaggac gggtcgtgac ctcgtcagaa gccggcggcc atgccttcct tgcgatggtc
     2641 tgaccccgca atgtaggcgt cttccgactc gtttcggtag atgagctgag caccgccgaa
     2701 ctggtgttct tgatcttcga caccgatctg atggccgcgg tcggccagct ctttgatgac
     2761 tttctccggg aattccggct ccacccgcag gctgtagtcg ggaaagaccc gccagcgggg
     2821 agcgtcgctg gcggcctggg ggttttgacc gtgatcgaat attcgctgca ccatctggaa
     2881 gtgtccctga tgctgcatgt ggccgcccat gacgccgaac gccagccggg ggcgcccgtt
     2941 ttcggtcacg aatcccggga tgatggtgtg gtacgggcgt tttcgggggc ctacacggtt
     3001 cgggtgcccg ggttccaggg tgaaccctga tccgcggttc tgcatggaaa tgccggtgcc
     3061 gggaatcacg atgccggatc cgaaccccat gaagttggat tggatgaacg agaccatcat
     3121 gccgctcgca tcggcggcgc agaggtagac ggtatcctgg ctggttggga gcgacacggg
     3181 cgggagcgtc gacgcgctcc tgccgatggt cccggcggcg cggcgcagat ttgccgggtc
     3241 cagcagttct gccggcggca ggcgcatggc gtcggcgtcg gcaaagtgat cggccgccgc
     3301 ccggatcgca atcttcatgg cttcgacctg gaggtggacg ctttcggccg tttcgagcgg
     3361 gggcgcgtcg aaatgggcaa agatgcccag ggcgatctga gcggcaaggc cctggccgtt
     3421 gggtggaatc tcgtggacgt cgacgctgcg gtaaggctgg gagagcgggt ccacccactc
     3481 ggcgcgatgc gcagccaggt catcccgggt catgacccca ccgtcggtgg cggccgcggc
     3541 cgcgattcgc tcagcgatat cgccttcgta gaaggctttc ccgcccgtgg ctgcgatttc
     3601 ctgaagcgtt ctggcgagat ccgggcgccg aaaccgctca ccggcgcgag gcgcgggcag
     3661 gaagtgattg gcgaaatccg gaaagtccgc gtagcggcgc tcggcaaact gccagtagta
     3721 ggccgttttg gggccaacgg gaaacccttc cgcggcatag cggatggcgg cgtcgaacag
     3781 atcgggaaac gcgagccggc cgtagcgctc cgacagggcc gaccaggcgc tgaccgcgcc
     3841 cgggaccgtc acggcgtccc atccgagctc gggcattgtg cgctggccgg cgaagcggtc
     3901 gggcgtccag ccggcgggag agcggcccga agcgttcagg ccgacgagtt ttgaaccgtc
     3961 ccacacgatg gcgaaggcgt cgctgcctac gccgttgttg ttgggttcca ccacggtcag
     4021 cgcgatggcg gttgccaggg ccgcgtccac cgcgttgccg ccgctgcgca tggcgtcgag
     4081 gccggcctgc acggccagcg gttgagagct ggccacgatg ttctcggcgc aaacggccga
     4141 gcgagcggcc cgatacggct gttcccaatc catcagcaga cgttactgcg ttgcgtgaat
     4201 cgggaaaagc ccctaacttt ggctgcctgc atatcgggcg caatccaagg gagacagcgt
     4261 gcatccggga atcatcgcgg cggccaaccc agacaaggtt gcctacctca tggccgactc
     4321 gggcgagacc atcacttacg gcgagctgga gaagacttcg aatcagggcg cgcacctgtt
     4381 tcgacagctc gggctgaaaa ggggcgatca catcgcgttg ctgctggaaa accacccgcg
     4441 gttctttcag atctgctggg cggcccagcg tgcgggcctt tactacacgg ccataagctg
     4501 gcgcctgcag caggcggaag tggaatacat cgtcaacaat tgcgaggcgg ccgtgttcat
     4561 cacctcggct gcacggcgcg aggtggttgc gccgctggcg gacagtatgc cgggtgtctc
     4621 ccatcggttc gtcatcgacg gagccatcga gggtttcgac gactgggccg aggcgatctc
     4681 cgccatgccg gtcacccccg ttgccgacga aagcgaaggg atggcgatgc tctactcctc
     4741 ggggacgacg ggttacccga aaggggtaaa aagacccctt ccggacggag agtatggggc
     4801 ggcggctggt atcaacctgc tgaacattct ctacggcgcg gacgatgatt ccatatatct
     4861 ttcgccggca cccctgtatc acgcggcccc gctcggcttc accatgggat gcctgcgcta
     4921 cggcattcag gtcgtcgtga tggagcattt cgaggcggaa cttgcgctcc gttacatcca
     4981 ggactaccga atcacccaca gccagtgggt gccgaccatg ttcgtgcgca tgctcaagct
     5041 gcccgaggag atccggctgc gcttcgacac gtcgacgctg cggtgcgcga tccacgccgc
     5101 cgcgccctgc ccggtgccgg tcaaggagca gatgatcgag tggtggggtc cggtcatcta
     5161 cgagtactac gccggcacgg agggtaacgg cttcgtgcag ctgaacgcta ccgagtggct
     5221 ggagcacaag ggatccgtcg gacgtccgct caactgcgag atccacattt gcggcgacga
     5281 cggcgaagag ctgcccgccg gcgagtcggg cacgatctac ttcggcggcg gcggcgagtt
     5341 cgagtaccac ggagatccgc agaagacggc cggctcccgt catcccaagg ggtggagcac
     5401 gctgggcgat gtcgggtatc tcgatgagga cggctacctt tacctcaccg atcgcaaaca
     5461 tttcatgatc atctccggcg gcgtgaacat ctacccccag gaggcggaga acgtgctcat
     5521 cacccacgat gcggtgatgg acgtcgccgt gttcggcgtg ccgaatccgg agttcggaga
     5581 ggaggtgaag gccgttgttc aacctaccga catggcgctg gcgacccctg agctcgcggt
     5641 ggagctgatg gactactgcc gtgcccggct atcccacatc aagtgcccgc gctcggtcga
     5701 cttccgggag gagttgcctc gacaccccac gggaaagctt tacaagcggc tgctcaagga
     5761 cgagtactgg agcaaggcgg gaggctgagg gcgccgctca gcggtcgcgc agcaggttgg
     5821 ccggcgcgta ctggtccgtg agcacccttg ccgacgtgtc ccagtcgggt cgggtctcca
     5881 gccctttgac gtgatcgctg agactaatgc cgtacttgcg cacccgcggt gccagatcgg
     5941 ccgcacgctg gttcattatg gtggggtccg gtaggggacc gttcgcggcg agaattatgc
     6001 ggttggcgct ctcgccggtc ttgaggttgt agaaacgtcc gaacacggcc gtgtaggtcg
     6061 tggattcgtg atgatacagc tggctgatgg aaaacgtgtt ggccacaacg gctccgccgg
     6121 gcgtcagcag ggaccttgtc tcctgcagaa actcgcgcgt catcagatgt tccggaatgt
     6181 agtcgccgtt gaacgcgtcg agcatgatga gatcgtaggt atccccgcga agcgcggccc
     6241 gcttgatgaa gacgcggccg tcctgggtaa agacggtggt gttggcggaa ggaacatagc
     6301 cgaagaactc gccggctact cgaacgacgg cggggtcgat ctcgaccacg tcgatctcgg
     6361 tgtcgggata gagttcggcc agggccgtgg gcagggtgcc gcctccgagc ccgatcatga
     6421 gtatcctttc cgggctcggc cggagcagaa gccccgtcat catcatgcgg gtataggaaa
     6481 ataccatctc gttcggccgg cgctggtcca tgcaggattg gttgcgctga tcggttctta
     6541 tcgagaactg caggcagacc agcgaaccct gctgatcgat gagaatcgtg ctgtacaggg
     6601 agcgctcccg atgcacgatg cggccctggg catcgccggc cggcaagagc agcacgaacg
     6661 tgaagaggcc ggctgcgatt cgcatcatga tgcttgccac ccccatcggt gcgcggtgac
     6721 ggcgatgatg cccatggcgg cgagggtggc ggtcatgagc gccaggatgg tgttcacttc
     6781 gaaccacagc acgaagtaga aggaagttgc cagggtgccg agggcgctgc cgagcgtcga
     6841 catgaagtag aggcggccgg ccacgtgacc cgactcgtgg gttgatgcga ccagcagacg
     6901 gaccgagtac ggtgagatca ttcccaggat gacggtggga acgatgaaca gcgcggagga
     6961 agcgagcagc gacccgtagc gcgggtcctc tatccgggtg aagatccaca ccatcgccgg
     7021 ctcggcaaag taaaccagag ggtagagcgt gactgcccc
//
LOCUS       DBNHLM010000020         3696 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1378999, whole genome shotgun sequence.
ACCESSION   DBNHLM010000020 DBNHLM010000000
VERSION     DBNHLM010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1378999"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="AC16QF_01055"
     CDS             complement(<1..572)
                     /locus_tag="AC16QF_01055"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR6447166.1"
                     /translation="MSESSTSTPYAGLTPDLILDALEATGFAPTGSLLELNSYENRVY
                     QLEVEDGTFVVTKFYRPERWSDAQILEEHAFTAELIEHELPAVGPLRRDGTTLFEHSG
                     FRFAVFPRQGGHPPNIEVEEDLKVHSRTLARLHAIGRIRPFETRPTLSCERMGRQSRE
                     FLLETNLVPLELQPAYASTTEHLLERLEPL"
     gene            complement(595..1290)
                     /locus_tag="AC16QF_01060"
     CDS             complement(595..1290)
                     /locus_tag="AC16QF_01060"
                     /inference="COORDINATES: protein motif:HMM:NF039185.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR6447167.1"
                     /translation="MIGRALRALFPPICLLCDQSCAAEADVDLCSRCLPVLPWNPQPC
                     PRCALPRENGTGRCQPCEDDPLPVARTVAPLLHAGYPRHWVHRLKFHEGMVEGRLLAH
                     LLAGAVRESYGRTDLPDMLVPVPTAPGRLAWRGHNQAATIAAFLSRRLSLPLARRGAR
                     RARSSRPQRLLSRAARLKATAGIFESRQWRGETIAVVDDVMTTGATVAELARTLTAAG
                     AGEVHVWSATRAV"
     gene            1497..3263
                     /locus_tag="AC16QF_01065"
     CDS             1497..3263
                     /locus_tag="AC16QF_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6447168.1"
                     /translation="MPASLKDVRFLIHDVFDFAGHYARLELDEPPGRDLIDAIVEEAA
                     RFADGELAPLNRVGDESGCRLEDGSVSTPAGFKEAYAKYVEGGWAGLSGSAAYGGQGL
                     PDSMSFFLEDLMCCANMAWTMYPGLSRGVVGALEEHGSAELKDAFLANLLSGEWTGTM
                     CLTEPHAGSDVGLARTRAEASADGTYRISGTKIFISAGEHDMAENIVHLVLARIPDAP
                     PGTKGISMFVVPKFDLDGTRNGVSCGSIEEKMGIHGNATCVLNFDGARGYLVGEPNHG
                     MRYMFTMMNGARLVVGLQGASQAHAACLLSLEYAQDRLQMRSLTGPKAPERPADPIIV
                     HPDVRRMLLFQRAIAEGGRALVYYAAQIIDRFRDGSPDERASAGELLDFLTPVVKGIL
                     TELGFESVNQAVQIYGGHGFIRETGVEQYVRDTRITTIYEGTTQIQALDLVGRKILQN
                     QGKGLVSFIGEMQAVAASLEDPLPRLAAPLKALADEWGELTLQLSATAMQDLDEVGAA
                     AVDYLYYSGYAALAFCWALMARSADEAIRAGSTDPFHAGKIAVADFFFSRVLPRAKAH
                     QAAIEAGKGTLMDVDEGALVAL"
     gene            3312..>3696
                     /locus_tag="AC16QF_01070"
     CDS             3312..>3696
                     /locus_tag="AC16QF_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR6447169.1"
                     /translation="MLEYQAPLRDFDFLLFDVFRAGETWGRIPAFAELSEELVRAVLA
                     EGGRVAREVMAPLNQTGDEQGCRWDDGVVTAPAGFKAAYDDMAGGGWLGISGNPEFEG
                     QGMPKMLGCLVEEMFWSANTSLFLYG"
BASE COUNT          618 a         1142 c         1290 g          646 t
ORIGIN      
        1 aacagcggct ccagccgctc gagcagatgc tcggtggtgc tggcgtaggc cggctgaagc
       61 tccaggggaa cgaggttcgt ttccagcaga aactcgcggc tctgcctgcc cattcgctcg
      121 cagctgagcg tggggcgtgt ctcgaacggg cggatccgac cgatggcatg aagccgggcc
      181 agcgtgcggg agtgaacctt cagatcttcc tcgacctcta tgttgggagg gtgcccgccc
      241 tgcctcggga acacggcgaa acggaatccg gagtgctcga acagggtggt gccgtctctc
      301 cgcagcggcc ccaccgcggg cagctcatgc tcgatgagct ctgcggtgaa cgcgtgctct
      361 tcgaggatct gcgcgtcgct ccagcgctcg ggacggtaga acttggtcac gacgaacgtc
      421 ccgtcctcaa cctccagctg atagacgcgg ttttcgtagc tgttcagctc gagcaggctt
      481 ccggttgggg cgaacccggt ggcctccaac gcgtcgagta tcaggtccgg ggtcaggccg
      541 gcatagggtg tgctggtgga cgactcgctc atgagagcca gtctaccagg gaactcagac
      601 ggcgcgggtg gccgaccaca cgtggacttc gcccgcaccc gccgccgtca gcgtgcgtgc
      661 gagctcggcg actgtagccc cggtggtcat gacgtcgtcg accacggcga tcgtctcgcc
      721 ccgccactga cgtgattcga agatgccggc agtggctttc aggcgtgcgg cgcgggacag
      781 aagtctctgg ggccgggagc tgcgggcgcg cctggctccg cgccgggcca gcggcaggct
      841 caggcggcgg ctcaggaaag cggcgatggt ggctgcctga ttgtgacccc gccaggccag
      901 ccgtcccgga gccgtgggaa cgggcaccag catgtccgga aggtcggtcc tgccgtagct
      961 ttcacggacc gccccggcga gcaggtgcgc cagcagccgg ccctcgacca tgccctcgtg
     1021 aaacttgagc cggtggaccc agtggcgcgg gtagccggcg tggagcagcg gcgccacggt
     1081 gcgggccacc ggcagagggt cgtcttcgca gggttggcat cgcccggtgc cgttctcccg
     1141 cggaagcgcg cagcggggac agggctgcgg gttccagggc aggacgggca ggcaccggct
     1201 gcacaggtct acgtctgcct cggctgcgca gctctgatcg cagagcaggc agatgggcgg
     1261 gaacagggct cgcaacgcgc gaccgatcat cggttgatcc tgcccgttgg acgtttggca
     1321 cgacagtagc gaatgtcctc gtatggcgtg cgaagcccgt cacgattcac ccgctcctcc
     1381 gggcgggagc aggccgcgat tttgcgcatc agcgcggggc gaacgcgatc tgtggagggg
     1441 gtcattgacg ggccgcgaag atttgctacc gtgccctccg cattggagga tccaagatgc
     1501 cagcatcgct caaagacgtg cggtttttga ttcacgacgt gttcgatttc gccgggcact
     1561 acgcacggct cgagctcgac gaaccaccgg gccgcgatct catcgacgcc atcgtcgagg
     1621 aagctgcgcg gtttgccgat ggcgagctgg cgccgctcaa ccgcgttggg gacgaatccg
     1681 gctgccgact ggaagacggg tcggtgagca cgccggccgg gttcaaggag gcctacgcca
     1741 agtacgtcga aggaggctgg gccggtctca gcggttccgc tgcctacggt ggccagggcc
     1801 tgccggacag catgagcttt ttcctggagg acctgatgtg ctgcgcgaac atggcctgga
     1861 ccatgtaccc gggcctttcg cggggggtcg tcggcgcttt agaggagcac gggagcgcgg
     1921 agctcaagga cgcgtttctc gccaatctcc tctcgggtga gtggacgggg accatgtgtc
     1981 tgaccgagcc ccacgccggg tcggacgtgg gcctggccag aacccgggcg gaggcctctg
     2041 cagacggaac gtaccggatc tcggggacca agatcttcat ctctgccggc gagcacgaca
     2101 tggcggagaa catcgtccac ctggttcttg cccgcattcc ggacgcgcct ccgggaacca
     2161 agggcatatc catgttcgtc gtgcccaagt tcgatctcga cggcacgcgc aatggcgtgt
     2221 cgtgtggctc catcgaggag aagatgggca tccacggcaa cgccacctgc gttctcaact
     2281 tcgacggcgc caggggatat ctggtgggcg agccgaacca cggcatgcgt tacatgttca
     2341 cgatgatgaa cggcgcgcgg ctggtggtgg gtctgcaggg cgccagccag gcccacgcgg
     2401 cctgcctgct gtcgttggaa tacgcccagg atcgcctgca gatgcgctcc ctcacggggc
     2461 ccaaggcgcc ggagcggccg gcggatccca tcatcgtgca cccggacgtg cggcgaatgc
     2521 tgctcttcca gcgcgccatc gcggaagggg gacgggcgct cgtctactac gccgcacaga
     2581 tcatcgatcg tttccgggac gggtccccgg atgagcgggc ttcggccggc gagctgctcg
     2641 attttctgac ccccgtggtc aagggtatcc tgacggagct tggcttcgag tcggtcaatc
     2701 aagccgtgca gatttacggt ggccacggct tcatccggga gaccggtgtc gagcagtacg
     2761 tgcgcgacac cagaatcacc accatctacg agggcaccac gcagatacag gccctcgatc
     2821 tcgtgggtcg gaaaattctg cagaaccagg gcaaggggct cgtcagcttc atcggcgaga
     2881 tgcaggccgt tgccgcctcc ctggaggatc cgctgccccg tctggccgct cctctcaaag
     2941 cgcttgccga cgagtggggc gagctcacgc tgcagctcag cgcgacggcc atgcaggatc
     3001 tggatgaggt aggtgctgcg gccgtggact acctctacta cagcgggtac gcggcgctcg
     3061 ctttctgctg ggctctcatg gcgcgcagcg cggacgaggc gattcgagcg gggtcgacgg
     3121 atccctttca tgcaggcaag attgcggtcg ccgacttttt cttcagtcgc gttctgccgc
     3181 gcgcgaaggc acaccaggct gccatcgaag cgggcaaagg gactctcatg gacgtcgacg
     3241 aaggtgcgct ggtcgcgctc tgaacaccgt gcgcttgaag tcatgattgc cttggacggc
     3301 agggggaacc catgctcgag tatcaggccc cgctgagaga cttcgacttt cttctgttcg
     3361 atgtgtttcg ggcgggggag acctggggcc gtattcccgc atttgctgag ctatccgaag
     3421 aactcgttcg cgccgtgctg gccgaggggg gaagggtcgc cagagaagta atggcaccgc
     3481 tcaaccaaac cggagacgag caaggctgtc gttgggacga cggcgtcgtc accgcgcccg
     3541 cgggcttcaa agccgcatac gacgacatgg ccggcggcgg ctggctgggg atctcgggca
     3601 atccggagtt cgagggtcag ggcatgccga agatgctcgg atgtctggtc gaggagatgt
     3661 tctggtccgc aaacacgagc ctgttcctgt acggca
//
LOCUS       DBNHLM010000021         2666 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1558709, whole genome shotgun sequence.
ACCESSION   DBNHLM010000021 DBNHLM010000000
VERSION     DBNHLM010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1558709"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1567)
                     /locus_tag="AC16QF_01075"
     CDS             complement(<1..1567)
                     /locus_tag="AC16QF_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447170.1"
                     /translation="MTPTDSGPATLRRFLPWLLPTALLAGHAAAADVRIGVDWNGDGL
                     IQTLPGSRVPQDAPSDSQPFVFWVNHDQDDLETGGETWPIARPDANTPAPDSSRDLED
                     FTRLHVEIDDLGSLPGDAVLTLAWASGSTAELNLFRNEDPDCSRRYLLDAEAGRRQLS
                     APHGTPIGTVGANALKIRLQTLGEPGLDGAGYCFLLEVASPGQGHLVATIADGEGVLA
                     RSEPIAAELRHIKSLYQRTTMTWPEEILAPWAYTTEAPPVPDLQWSKDPQGHEFEPGW
                     WETEDHVVWVYGWLKSGEGLREMATTQAAETIFKRLWHRGYRGRLTFFHWPTVKPRYA
                     YGLLESEYRAYKSAPALVDFVSQIPKGKRIHVTAHSLGCVLVTEAIKLGLEAEEALLQ
                     VGAIPAAAYDTRPVLTLPDMADVVTPWLPEEGGYHGYIDETPTPVYTMYNYADVTFFG
                     WNIAQKQMKPTAKRGGYRYAWEPDAPQGERARLYYKTGWFSSKYRLVDDPHEIMAFIA
                     KAKTHAIGAETRVQ"
     gene            complement(1590..2369)
                     /locus_tag="AC16QF_01080"
     CDS             complement(1590..2369)
                     /locus_tag="AC16QF_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR6447171.1"
                     /translation="MRHWPPSRTSPIGAADIIFKAMLVPAVLLAVAACTREAEPRWLL
                     RALPGEYPEIVYFVPTTSRAVAMTIDDGLDPETTPLILETLKAHGASATFFVLSDSIA
                     GREVLLQRILYEGHEIGHHMTEDEVTVSLKPAAMAEKFHRAAQTLDAFAPITWFRPGS
                     GRYNDAVLELTRARGYRIALASVAPLDTVIHNSARMAGFVERMIEPGSVVVLHDVGER
                     GRRTAETLKLLLPKLKERGYAVVSLSRLDALAGMDTSATEE"
     gene            2465..>2666
                     /locus_tag="AC16QF_01085"
     CDS             2465..>2666
                     /locus_tag="AC16QF_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447172.1"
                     /translation="MNFGSLLVRPDRPTGKLAIPRPGARLCGFAKRWKSAAGSALVRI
                     QEPAEEEQPDAQAFTDSNCARIW"
BASE COUNT          420 a          848 c          900 g          498 t
ORIGIN      
        1 cctgaacgcg ggtttcggcg ccgatcgcgt gggttttggc cttggcgatg aaggccatga
       61 tctcgtgggg gtcgtcgacc aaccgatatt tggagctgaa ccaccccgtc ttgtagtaga
      121 gccttgcgcg ctcgccctgc ggtgcgtcgg gttcccaggc gtagcgatac ccgccccgct
      181 tggccgtcgg cttcatctgc ttctgcgcga tgttccagcc gaagaaggtg acgtccgcgt
      241 agttgtacat cgtgtacacc ggggtcgggg tctcgtcgat gtaaccgtgg tagccgccct
      301 cctccggcag ccacggcgtc accacgtccg ccatgtcggg cagggtgaga accgggcggg
      361 tgtcgtaggc ggcggccggt atggcgccga cctgcaggag cgcctcctcg gcttccagac
      421 ccagcttgat tgcttcggtg acgagcacgc agccgaggct gtgggcggtg acgtggatgc
      481 gtttgccttt cgggatctgg ctgacaaagt cgaccagggc gggggcggac ttgtaggcgc
      541 ggtattcgct ctcgaggagc ccgtaggcgt agcgcggctt caccgtgggc cagtgaaaga
      601 acgtaagccg cccccggtag ccgcggtgcc agagccgctt gaaaatcgtc tcggcggcct
      661 gcgtggtggc catctcccgc aatccctcgc cgctcttgag ccagccgtac acccagacca
      721 cgtgatcttc cgtctcccac cagccaggct cgaactcgtg gccctggggg tccttgctcc
      781 attggagatc gggtaccgga ggcgcctcag tggtgtacgc ccatggcgcc aggatctctt
      841 ccggccaggt catggtggtc cgctggtaca gggacttgat gtggcgaagc tccgcggcga
      901 tgggctcgga acgggccagg acgccttctc catcggcgat ggtggcgacc aggtgcccct
      961 ggccgggcga cgcgacctcc agcagaaagc agtatccggc gccgtccaaa cccggctcgc
     1021 cgagggtttg cagccggatt ttcagagcgt tggcccccac ggtcccgatg ggcgtgccgt
     1081 ggggtgcgct cagctgccgg cggccggcct cggcgtcgag caggtagcgt cgtgagcagt
     1141 cggggtcttc gttgcggaag aggttcaatt cggcggtgga gccgctcgcc caggcgagtg
     1201 tcagaacggc atcgccgggc agcgatccga gatcgtcgat ctccacatgc aggcgcgtga
     1261 agtcttccag gtcccggctc gaatcgggag ccggggtgtt ggcgtcgggc ctggcaatgg
     1321 gccaggtttc tccaccggtc tccagatcgt cctgatcgtg gttgacccag aacacgaaag
     1381 gctgcgaatc cgagggggcg tcctgtggca cgcggctgcc gggcagggtc tggatcaagc
     1441 cgtcgccgtt ccagtcgacg ccgatacgga catcggccgc cgcggcgtgc ccggcaagca
     1501 aagccgtggg gagcagccag ggcaggaatc gtctcaacgt cgccggtcca gagtcggtcg
     1561 gtgtcacggt ttcggtcctg cttttcgcgt cattcctcgg tggcactggt atccatcccg
     1621 gcgagcgcat ccagccggct caggctgacc accgcgtacc cccgttcctt cagtttcggt
     1681 agcagcagct ttaaggtttc tgcggttctc ctgccccgct cgcccacgtc gtgaagcacc
     1741 accacgctgc cgggctctat catccgctcg acgaagcccg ccatgcgggc cgaattatga
     1801 atcacggtgt ccagcggtgc caccgaggcc agcgcgatcc ggtagccccg cgcgcgcgtc
     1861 agctcgagca ccgcgtcgtt gtaacggccg gagcccggcc gaaaccaggt gatgggcgca
     1921 aacgcgtcga gcgtctgcgc ggcgcgatgg aatttctctg ccatcgcagc cggtttaagg
     1981 gagaccgtga cctcgtcctc ggtcatgtgg tggccgatct cgtggccctc gtagaggatt
     2041 ctctgcagca gaacctcgcg gccggcaatg ctgtcgctca ggacgaagaa cgtggctgag
     2101 gctccgtgag ctttgagcgt ctcgaggatc agaggcgtgg tctcgggatc cagaccgtcg
     2161 tcgatagtca tggccaccgc ccgggaggtc gtcggcacga agtagacgat ctcgggatac
     2221 tccccaggca gcgcgcggag cagccaccgt ggctccgctt cccgcgtgca ggcggcaacg
     2281 gccagcagca cggccggcac cagcatcgct ttgaagatga tgtctgctgc gccgatgggt
     2341 gacgtgcggc tcggcggcca gtgtctcatc aggattgcgt ccggacttcc tgggagtggc
     2401 gagtgtgatt cgacggctct ctatcgtgtc agagtcgccg tcttcatgaa accggccccc
     2461 cgtcatgaac ttcggctccc tgctggttcg gcctgaccgt cccaccggca aacttgcaat
     2521 cccgagaccg ggcgctaggc tgtgcggttt tgccaagcgt tggaaaagcg cagcgggatc
     2581 ggccctggtg cgcattcagg aacccgcgga agaagaacaa ccagacgctc aggccttcac
     2641 cgactctaac tgcgcacgga tttgga
//
LOCUS       DBNHLM010000022        13198 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1199704, whole genome shotgun sequence.
ACCESSION   DBNHLM010000022 DBNHLM010000000
VERSION     DBNHLM010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13198)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13198)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13198
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1199704"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="AC16QF_01090"
     CDS             complement(<1..661)
                     /locus_tag="AC16QF_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6447173.1"
                     /translation="MAGTQATLYETRKGAAWITLNRPANRNALSAPLVNELYDHLLVA
                     NDDAAVRSIVITGTGPAFCAGADLKEPPGQAIDGGRAVPYADVLSAILDSPKPVIAAV
                     NGAAFAGGLGLIGAADIVVTAEDVQFSFSEVRIGVIPAVISVVCLPKLGVHHGMKLIL
                     TGERFSGRRAVEIGFAHRAVPADELVDAVQEEIDMINLGGPNAMVECKKLVRRVPRLT
                     VE"
     gene            complement(677..2650)
                     /locus_tag="AC16QF_01095"
     CDS             complement(677..2650)
                     /locus_tag="AC16QF_01095"
                     /EC_number="6.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HIR6447174.1"
                     /translation="MADVSRPISRILVANRGEIARRIIRTAHELGIGTVAVYADADAD
                     APYVREADTAIALGGRSSTETYLDIPKVIEACRQSGADAVHPGYGFLSENERFAQAVV
                     DEGVRWIGPAPRVIGLMGDKLSAKRLMVEAGVPTLPAVEITEATDLAEAASQIGYPVL
                     VKASAGGGGRGMRVVEDEGGLADAVLGAKREAASSFGDDTVFFEKWLESSRHVEVQIL
                     GDGHGNVLHCFERECSIQRRHQKIIEEAPSPAVTPEIRDRMGAAAVAAAKKLGYTSAG
                     TVEFLLSGDEFYFLEVNARLQVEHPVTEAVIGWDLVREQIRVAEGERLSFTQEELAIS
                     GHAIQARLYAEDPGRDFLPSPGTVVAWAPSRAGSARFDSGVESGSVIGTEFDPMIAKV
                     IVHAPTRREAATRLARVLETTRIQGLTTNRDFLVATLRTPEFLAGDTTTDFIERVRPA
                     VVRCIGRDELVNAAIAAAIEGRDARRKSARVMGTIPGGWRNSTMPMETVGYSAGAGEV
                     QLRYRARRDGMFRFVGDESEKLVRVHGAGGAETDLEINGQRLRFLVGRLGDTWMVHGS
                     SGNVELVELPRYPLAGGGDLGSGMLAPMPGRVLETQVAEGTEVVKGQLLLVLEAMKME
                     HRITAPRDGVVAKVHVNAGDQVENGQLLVTLAE"
     gene            complement(2638..4269)
                     /locus_tag="AC16QF_01100"
     CDS             complement(2638..4269)
                     /locus_tag="AC16QF_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR6447175.1"
                     /translation="MPVLESSLDTRAEAFVRNKADMQQALEEFRALLDEAAEGGGAEA
                     AARLASRGKMPIRERISFVLDRDSPFLEISPLAAWRSAYAVGSGFVVGIGVIEGVECV
                     ILGHDPSVRAGAFNPFNSKKLMRGLEIARINRMPYVQFVESAGADLRGETGEDPEAAI
                     RREVDHFAESGRLFYEITELSKMRIPTISVVFGASTAGGAYQPGMSDYNIFIKNQSRV
                     FLGGPPLVKMATGEDADEESLGGADMHTQVSGLGDYLALDELDGIRMCREVVKHLNWR
                     KLGPEPRADFAEPLHDPEDLLGMVSKDLRSPFDMREVIARIVDASYFEEFKPLYGPTL
                     VCGWASIHGYPVGILGNNGALFSESSEKAAQFIQLCNQIDVPLLFLHNITGFIVGTDF
                     EQGGITKNGSKMINAVANSTVPHITVIVGASYGAGNYGMSGRAFGPRFTFIWPFAKIA
                     VMGPKQMAGVMSIVGRAAAARRGIEFDEEADASRAAVAEHWAEERSKGLYATSRVSDD
                     GMIDPRDTRTVVAIALSACFNNRVEGTKEFGTWRM"
     gene            4384..5583
                     /locus_tag="AC16QF_01105"
     CDS             4384..5583
                     /locus_tag="AC16QF_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6447176.1"
                     /translation="MTTDQQLADFEHQVDGWFAENKPADPGFLLPETFMEVGTDQQFE
                     FLRDWQRKVYEAGYVGMAWPKAYGGGGLPQIYQDIATRAMARHRVPFLPNTIGLNWAG
                     PLILHMGSEEDKQRYIKGILSGDDIWCQGFSEPDHGSDLGNAQCRATRDGDEYVINGS
                     KIWTSLGTYAKYMILLARTATGGSSKYAGLSFFLCPMDAPGIEPRPIQKLTGEYGFCQ
                     TFFDEARIPATCLMGQEGDGWRIAMITLTFERGATGGQAGGLSAMDLNINDVVELARR
                     ARRDGGPALADPLIQDELVQLMIESRGNALMADRTTIPALASDWPEAIAMSGKLRATE
                     LKRRMCRFALAMQGGNGARYVSADAVDGGMWQRSYLNAFSGTIGGGTSEIQRNILGER
                     VLGLPKD"
     gene            5592..6716
                     /locus_tag="AC16QF_01110"
     CDS             5592..6716
                     /locus_tag="AC16QF_01110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6447177.1"
                     /translation="MASIEFSDEQSLLLETAASFCRDRSPIERVRAGLGDENGCDAAV
                     WQEMVDLGWLGIAAPLEHGGSGLGLAGVVPVVECMGRHLMGSPYVATIQAIEALLGSA
                     SDEQLAQWLPTILAGAPATVALMEPDGSWDVRAATASGESTGNAIRLRGTKTFVLDAA
                     AAELIVTSIDLDGETRLVALRRDQLPDGALNRETVIDETRRSYGLVLDGIRVPRSQIL
                     PGSDFERIERAALLLVCAEIAGGLCGVLHTIVGYLTTRRQFDRLIGSYQALKHPTAEI
                     LLAAERSKSHLYHAATTIGRGGREAEVALRMAKAQGSEAFAWAGDRAVQFHGGFGFTY
                     ECDAQLFLRRALWCQYQLGDERYHRRKLAPLLLAATDTEH"
     gene            6721..7722
                     /locus_tag="AC16QF_01115"
     CDS             6721..7722
                     /locus_tag="AC16QF_01115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6447178.1"
                     /translation="MKAITSITGIDLNEVGPLTRDLEARGFSGVSTQENRFNPFLPLA
                     VAATQSSTLELRTSVAIAFARSPMVSANLGWDLQAASRGRFTLGLGSQVKGHNVRRFS
                     VPWSPPAPRLREYVRALRAIWDCWQNGTPLDFAGEHYQFNLMTPNFTPPPLATAPPRV
                     QLAGVGPATMKVAAEECDGVMLHGFCTRRYVEEAVMPRLETGLAAAGRAREDFEISGG
                     GFVATGKDDAAVAEMFEWVRTRVGFYGSTPSYWPVFEVHGLEDLGHKLNDMSKLGQWD
                     AMAGEISDDVVRLFAAVGRFDEIAGAIEERFGGAVDVVALPASAPPDLLEDIARIAC"
     gene            complement(7751..8485)
                     /locus_tag="AC16QF_01120"
     CDS             complement(7751..8485)
                     /locus_tag="AC16QF_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HIR6447179.1"
                     /translation="MAHEIDYRICGESMQYLEVELDPGESVVAEAGTMMYLSEGIAYE
                     ARLGDGSASGVVGKLFGAAKRLLGSESLFMTHFTNEADGPRQMGFAAPYPGQVVAVDL
                     AQAGGEIVCQKDAFLCAEMGTSVSVAFSKRLGAGFFGGEGFILQRLAGDGTAFIHAGG
                     TVLEKRLEGEKLRVDTGCIVGFEPGIDYDIQLAGGLKSMMFGGEGLFLATLEGHGRVW
                     LQSLPFARLADRVLENAVHLERKGES"
     gene            8642..9877
                     /locus_tag="AC16QF_01125"
     CDS             8642..9877
                     /locus_tag="AC16QF_01125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6447180.1"
                     /translation="MKTKIFDRFLTNGTLAIVDADGSSRQFGCGAPFATMRLNRRGTL
                     TRILRNPELNLGETYLAGEWDVIEGSLHDLLTILRTNLERHAGRSRLARALALMLVPW
                     NHVAASHRNVRHHYDLDETLFRACLDTNMHYSCAYYREANMSLEEAQQAKCDHIAAKL
                     CLRPGMRVLDVGCGWGSLAMELAERHDVTVMGVTLSPEQLRVARSTARLRGLDNHVRF
                     ELQDYRQVEESFDRIVSVGMFEHVGRRHYDEFFGKVEALLADDGVALLHTIGASSPPS
                     VVNPWIERHIFPGGYIPSLSDVAPAVERSGLITADVEVLRHHYALTLQEWNRRFQQRR
                     AEFAQSKGEAFCRMWEFYLLACQTAFEVSDLVVHHWQLAKRNLSVPTTRNYLYETQPY
                     VKIPGETAAEEITLRQHQG"
     gene            complement(9868..10815)
                     /gene="glk"
                     /locus_tag="AC16QF_01130"
     CDS             complement(9868..10815)
                     /gene="glk"
                     /locus_tag="AC16QF_01130"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HIR6447181.1"
                     /translation="MADGAYLVADIGGTNARLALTDGTRLTSPVLISQTASLRNVAGL
                     AEAARSAFDGQALDGCCIAMAGPVHANQGSLTNVSLAVSGDALGAELGCPARVVNDFY
                     AVARGIPFLRHLRQIGPGEPDEGVRALLGPGSGLGMAVLAPTREGPPLVLPSEGGHAD
                     LAPGSPLEQEVLQVLAARLPHVSWESVLSGPGLVNLYCALCAIWGVPEEALAPEEISR
                     QGLVVEDPICHQVLEMFFGFLGAAAGNLALTVCAEGGVFIGGGIVPKLVEFAESSPLR
                     RRFEERGVLADYAARLPLYVIEDEFPGLLGAVACLADQP"
     gene            complement(10815..11888)
                     /locus_tag="AC16QF_01135"
     CDS             complement(10815..11888)
                     /locus_tag="AC16QF_01135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6447182.1"
                     /translation="MEDRRYLVVGARGLVGRAVLSALEAEDRAEVIGLSRRQPDFPTR
                     ARWIPCDLTDRDACHAALGAAQTQGVTHIVYAALQEMPDLISGWRDPVQIETNARMLA
                     NLLEFVQPREHFTLLQGTKAYGAHLAPMALPGKESAPRHPGENFYWEQEDLMRSRQAG
                     SSWAFSIVRPQIVCGVALGSPMNMVAAIGVYAAVMRQLGKPLCFPGGGGFVTEATDAD
                     LLARAIIWAGREPACANEILNVTNGDVLDWVSLWPAFGEIFQMPTGEADPMRLAISMP
                     ELSGVWDEVVGRHGLKSMAMAELVGDSWQFADAVFGSGGGARSTLLSTIKIRQLGFHE
                     CIDTEQMFRNQLAALQDLKVLPR"
     gene            11983..12909
                     /locus_tag="AC16QF_01140"
     CDS             11983..12909
                     /locus_tag="AC16QF_01140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6447183.1"
                     /translation="MTVSAGLGIANFPFQDGPDFWRWVDLCDAGGVDSIWQSDRIISR
                     DPQLECMSVMAALAGGSRRLKFGMNVASLALRDPVLTAKACATIDVLSDGRLLPAFGV
                     GSALSRDFTATGTPTRGRGKRTAEGLEIMARLWSEESVSFHGEFYQLDDASISPRPVQ
                     QPLPLWVGGSAKQAIERTARWGTGWQAGIESAEEVAPVIAAIKAEAAQLGRSIDEDHF
                     GAGFGFRFGSPDEPICTRYLELLRKRLGREPKGFLAVGGVPEIMGLLHEFRDAGVHKF
                     ILRPVATDGEDFVDQTRRFIEHLLPEVSALNG"
BASE COUNT         2301 a         4522 c         4207 g         2168 t
ORIGIN      
        1 cttccacggt cagccggggc acgcggcgca cgagcttctt gcactcgacc atcgcgttcg
       61 gaccgccgag attgatcatg tcgatctctt cctgcaccgc gtcgaccagc tcgtctgccg
      121 ggaccgctcg atgggcgaag ccgatctcca ccgcgcggcg ccccgagaac cgctcgccgg
      181 tgagtatcag cttcatcccg tggtgcacgc cgagcttggg caggcagacc acggagatga
      241 ctgccgggat gacgccgatg cggacctcgc tgaagctgaa ctgaacgtct tccgcggtca
      301 ccacgatatc ggcggccccg atgagaccga ggcccccggc aaacgccgcg ccgttcacgg
      361 cggcaatgac gggcttcggg ctgtcgagga tggcgctgag aacgtccgca tacggcaccg
      421 cgcgaccgcc atcgatggcc tgaccgggcg gctccttcag atccgctcca gcacagaagg
      481 ccgggccggt cccggtaatg acaatcgaac gcacggcggc gtcgtcattg gcgaccagaa
      541 ggtgatcgta gagctcgttc accaggggag ccgagagcgc gttgcggttc gcgggccggt
      601 tcagggtgat ccaggccgcc cccttgcgcg tctcgtaaag tgttgcttgg gtgccggcca
      661 ctgggttcag ccctccttat tccgccaggg tcacgagcag ctgcccgttt tccacctgat
      721 cgcccgcgtt aacgtgaacc ttcgccacga ccccatcccg cggcgccgtg atgcgatgtt
      781 ccatcttcat tgcttccagg accagcagga gctgtccttt gaccacttcc gtcccttcgg
      841 ctacctgggt ctccagcacg cgcccgggca tcggcgcgag catgcccgag cccagatcac
      901 cgccgccggc tagcggatag cgcgggagct ccaccagctc cacgtttccc gagctgccgt
      961 gaaccatcca ggtatctccg agccgcccaa ccaggaatct caggcgctgg ccgtttatct
     1021 ccagatccgt ttctgcgccg ccggctccgt gaacgcgaac cagcttctcc gattcgtcgc
     1081 ccacgaaccg gaacatcccg tcacgacgcg cccggtagcg tagctgcact tccccggctc
     1141 ctgccgagta gcccaccgtc tccatgggca tggtggagtt ccgccagccg ccgggtatgg
     1201 ttcccatgac ccgcgcgctc ttccggcggg cgtctcgccc ttcgatggcg gcggcaatgg
     1261 ccgcgttcac cagctcatcc cggccgatgc atctgaccac tgccggccga acgcgctcga
     1321 tgaaatcggt ggtggtgtcg ccggccagaa actccggcgt ccgcagcgtc gccaccagga
     1381 aatcccggtt ggtggtgagc ccctggatgc gcgtcgtctc cagcactcgc gccagccggg
     1441 tcgccgcttc ccggcgtgtc ggcgcgtgca cgatgacctt tgcgatcatg ggatcgaact
     1501 cggtgccgat gaccgagccg gactccaccc cggaatcgaa acgggcgctg cccgcccgtg
     1561 acggcgccca ggcgacgacg gtgcccggtg acggcagaaa gtctcggccg gggtcttccg
     1621 cgtaaagccg cgcctggatg gcatggccgg agatggcgag ctcttcctgg gtgaaggaaa
     1681 gccgctcccc ctcggcaaca cgaatctgct cccgcaccag atcccaaccg atcacggcct
     1741 ccgtgaccgg gtgctccacc tgaagccgcg cgttcacctc cagaaagtag aactcgtcgc
     1801 cgctcagcag aaactccacc gttccggcag acgtgtagcc gagcttcttt gcggccgcca
     1861 ccgcggccgc acccatgcgg tctcgaattt ccggagtgac cgccggcgag ggcgcttcct
     1921 cgatgatctt ctggtggcgc cgctggatgg agcactcgcg ctcgaagcag tgcagcacgt
     1981 ttccgtgacc atcaccgaga atctgcacct ctacgtgtcg cgagctctcc agccacttct
     2041 cgaaaaacac cgtatcgtca ccgaaggagg acgcggcttc gcgcttcgcg cccagcaccg
     2101 cgtcggccag cccgccctcg tcctcgacga ccctcatgcc ccgcccgccg ccgcccgcgg
     2161 aagccttcac cagcaccggg tagccaatct gcgatgcggc ttccgcaaga tccgtcgcct
     2221 cggtgatctc caccgcaggc agggtaggca cgccggcttc taccatgagc cgctttgccg
     2281 acagcttgtc ccccatgagc ccgatcacgc gaggggccgg cccgatccac cgcaccccct
     2341 cgtccaccac ggcctgggcg aaacgctcgt tctccgagag aaatccatac ccggggtgca
     2401 cggcgtcagc gccgctctgg cgacaggctt cgatgacctt tgggatgtcg agataggtct
     2461 cggtactgga acggcccccg agcgcaatgg ccgtatccgc ttcgcgcacg tagggcgcat
     2521 ccgcgtccgc atccgcgtac acggcaacgg tgccgattcc gagctcgtgg gccgtgcgga
     2581 tgatgcgacg ggcgatctcg ccgcgattag cgacgaggat ccgcgagatc ggcctgctca
     2641 catccgccac gtgccgaact ccttggtccc ttccacccga ttgttgaagc aggcagacag
     2701 ggcaatcgcg accacggtgc gagtgtcccg ggggtcgatc atgccgtcgt ccgagacccg
     2761 ggaggttgcg tacagcccct tggagcgctc ttcggcccaa tgctcggcga cggccgcccg
     2821 gctcgcatcg gcctcctcgt cgaattcgat gccacgccgg gccgccgccg cgcggccaac
     2881 gatgctcatc acgcccgcca tctgcttcgg gcccatgacg gcgatcttgg cgaacggcca
     2941 gatgaaggtg aaacggggcc cgaacgcgcg cccgctcatg ccgtagttgc ccgctccgta
     3001 cgaggcgcca acgatgacgg tgatgtgcgg aaccgtggag ttggcgacgg cattgatcat
     3061 cttcgaaccg ttcttggtga tgccgccctg ctcgaaatcc gtgcccacga tgaagccggt
     3121 gatgttgtgg aggaacagca gcggcacgtc gatctggttg cacagctgaa tgaactgggc
     3181 cgccttctcc gaagattccg agaagagcgc gccgttgttg cccaggatgc cgacggggta
     3241 gccatgaatg ctggcccagc cacacaccag ggtgggcccg tacaagggct tgaactcctc
     3301 gaagtaggag gcgtcgacga tgcgggcgat gacttcgcgc atgtcgaagg gcgagcgcag
     3361 atccttggag accatgccca gcagatcctc gggatcgtga aggggctcgg cgaagtcggc
     3421 tctcggctca gggccgagct tgcgccaatt gagatgtttg accacttccc ggcacatacg
     3481 gatgccgtcc agctcgtcga gcgccaggta atcgcccaac ccggatacct gggtgtgcat
     3541 gtcagcgccg ccgaggctct cctcgtccgc atcctcgccg gtggccatct tcaccagggg
     3601 cggtccgccg agaaagaccc gcgactggtt cttgatgaag atgttgtagt cactcattcc
     3661 cggctggtaa gcgcccccgg ccgtggacgc gccaaagacc accgatatgg tggggatgcg
     3721 catcttggaa agctcagtga tctcgtagaa cagccgcccg gactccgcga agtgatccac
     3781 ctcccggcgg atggccgcct ccgggtcctc cccggtctcg ccgcgcaggt cggcacccgc
     3841 ggactccacg aactgcacat agggcatgcg attgatccgc gcgatctcca gcccgcgcat
     3901 gagcttcttc gagttgaacg ggttgaaggc accggcgcgc acggaagggt cgtggccgag
     3961 aatgacgcac tccacgccct cgatgacgcc gatgcccacc acgaaaccgg acccgacggc
     4021 gtacgcggag cgccaggccg ccagcgggct gatctccaga aacggcgagt cacgatcgag
     4081 aacgaatgaa atccgctcgc gaatcggcat ctttccgcgt gaggccagcc gagccgcagc
     4141 ttccgctccg ccgccctcgg cggcttcgtc cagcagagcc cggaactctt cgagagcctg
     4201 ctgcatgtcg gccttgttgc gcacgaacgc ctcggccctg gtgtccaggc tcgattccaa
     4261 taccggcatg cggtcccccc gatccgtcga ggccacttta tgccagaact ttctcgtcag
     4321 aacaaaacgt gccgggagag gcgtttcgtt gtgctagatt gagcccgcga cgccggagga
     4381 gagatgacga cggaccagca actcgccgat ttcgagcacc aggtggacgg ctggtttgcc
     4441 gagaacaagc ccgccgaccc gggctttctg ctcccagaga ccttcatgga ggtcggcacc
     4501 gatcagcagt tcgagtttct ccgcgactgg cagcgaaaag tctacgaggc cggatacgtg
     4561 gggatggcct ggcctaaggc ctacggcggg ggcggcctcc cgcagatcta ccaggacatc
     4621 gcgacccggg ccatggcccg ccaccgggtt cccttcctgc cgaacaccat cggcctgaac
     4681 tgggcagggc cgctgatcct tcacatggga tcggaagagg acaagcagcg ctacatcaaa
     4741 ggcattctca gcggggacga catctggtgc caggggttct cggaacccga ccacggctcg
     4801 gatctgggca acgcccagtg tcgggcgacg cgcgacggcg atgagtacgt gatcaacggc
     4861 tccaagatct ggaccagtct cggcacctat gccaaataca tgatcctgct cgcccgaacg
     4921 gccaccggcg gaagcagcaa gtacgcgggc ctgagctttt tcctttgccc gatggatgcg
     4981 cccggcatcg agccgagacc catacagaaa ctcaccggcg agtacggttt ctgccagacg
     5041 ttcttcgacg aggctcgaat acccgccacc tgcctgatgg ggcaggaagg cgatggctgg
     5101 cgcatcgcca tgatcacgct cacgttcgag cgcggcgcga cgggcggcca ggccggcggg
     5161 ctgtccgcca tggatctcaa catcaacgac gtggtggagc tggccaggcg cgctcgtcgc
     5221 gacggcggtc cggcgctggc ggatccgctg attcaggacg agctggtgca gctgatgatc
     5281 gagtcccggg ggaatgccct gatggcagac cgcacaacca tccccgcgct cgcctccgac
     5341 tggcccgaag ccattgccat gtcgggcaag ctgcgcgcca ccgagctcaa acgccgcatg
     5401 tgccgattcg cgctggccat gcagggcggc aacggcgccc gctacgtgag cgcggatgcc
     5461 gtggatggcg gcatgtggca gcgctcctac ctgaacgcgt tctcgggcac catcggcggg
     5521 ggcacgtctg aaattcagcg caacatcctc ggagagcggg tactcggcct gccgaaggat
     5581 tgagcaccac cgtggcgtcc atcgaattct cagacgagca gtcgctgctc ctggaaaccg
     5641 ccgcctcgtt ctgtcgcgat cgatccccga tagagcgggt gcgcgccggc ctcggcgacg
     5701 aaaacggctg cgacgccgcc gtgtggcagg agatggtaga cctcggctgg ctggggatcg
     5761 ctgccccgct ggagcacggc ggttcagggc tcggtctggc aggcgtcgtt ccggtggttg
     5821 agtgcatggg ccgccacctg atggggtcac cctacgtcgc caccatccag gccattgaag
     5881 cgctgctggg ctcggccagc gacgagcagc tggcgcagtg gctgccgacg atactggccg
     5941 gggcgccggc aaccgtggcg ctgatggagc ctgacggcag ctgggacgtc cgcgcggcca
     6001 ctgccagcgg agagtcgacg ggaaacgcca ttcgcctgcg cggcaccaag accttcgtgc
     6061 tggacgcggc ggccgccgag ctgatcgtga cgtccatcga cctcgacggc gaaacccggc
     6121 tggtggccct tcgccgcgat cagctgcccg atggggcgct caaccgcgag acggtgatcg
     6181 acgagacccg ccgcagctat ggcctggtcc tggacggcat cagggtaccg cgatcccaga
     6241 ttctgccggg cagcgatttc gaacggatcg agcgggcggc cctgctcctc gtctgcgcgg
     6301 agatcgccgg cgggctttgc ggcgtgctgc acaccatcgt cggctacctg accacgcggc
     6361 gccagttcga ccgcctcatt ggcagctacc aggcgctcaa gcaccccacg gccgagatcc
     6421 tgctcgcggc cgagcgcagc aagtcacacc tctaccacgc agccaccacg atcggccggg
     6481 gcgggcgcga agccgaggtc gccctgcgga tggcgaaagc acagggaagc gaggccttcg
     6541 cgtgggccgg tgaccgggcg gtgcagtttc acgggggctt cggcttcacc tacgagtgcg
     6601 acgcccagct gtttctgcgc cgggcgctat ggtgccagta tcaacttggt gacgaacgct
     6661 atcatcggcg caagctcgcg ccgctactcc tagcagcgac agacacggag cattgagctc
     6721 atgaaggcca ttacctccat caccggcatc gacctcaacg aggtcgggcc cctgacccgg
     6781 gacctcgaag cgcgcgggtt cagcggcgtg tccacccagg aaaaccgttt caaccccttt
     6841 ctcccgctgg ccgtggcggc cacccagtcg tcgaccctgg agctgagaac cagcgtggcg
     6901 attgccttcg cgcgcagccc catggtttcc gccaatctcg gctgggacct gcaggcggct
     6961 tcccgcggcc gattcacgct cggcctcggc agtcaggtca aagggcacaa cgttcgccgc
     7021 ttcagcgttc cctggagccc gccggcgcca aggctgcgcg agtacgtccg cgctcttcgg
     7081 gccatctggg actgctggca gaacgggacc ccgctcgatt tcgccggcga gcactaccag
     7141 ttcaacctga tgacgccgaa cttcacccca ccgccgctgg ccaccgcccc gccccgggtg
     7201 cagctggccg gcgtcgggcc cgccacgatg aaggttgcag ccgaggagtg tgacggggtc
     7261 atgctgcatg ggttctgcac gcgccgatac gtggaggaag cggtgatgcc gcgcctcgag
     7321 acgggcctgg ccgccgccgg gcgcgcgcgc gaggacttcg agatctccgg cggtgggttc
     7381 gtcgccacgg gcaaagacga tgctgccgtt gcggagatgt tcgagtgggt ccgaacccgc
     7441 gtgggctttt acggttccac cccctcctac tggcccgtat tcgaggtgca cgggctcgag
     7501 gatctcggcc acaagctgaa cgacatgtcc aagctcggcc agtgggatgc catggccgga
     7561 gaaatatctg acgacgtggt ccggctcttc gccgcggtgg gccgtttcga cgagatcgct
     7621 ggtgccatcg aggagcgctt tggcggcgcc gtggacgtgg ttgccctgcc cgcctcagcc
     7681 ccgcccgacc tgctcgagga catcgcccgg atcgcttgct gagtccccct caggagccgc
     7741 cggatccggt tcaggactcg cctttgcgtt ccaggtgcac ggcgttctcc aggaccctgt
     7801 cggccagccg cgcgaacggc agggattgca gccatacccg tccatgcccc tccagagtgg
     7861 caagaaacag gccctcgcca ccgaacatca tggatttcag gccgccggca agctggatgt
     7921 cgtagtcgat gcccggctcg aagccgacga tgcagccggt gtccacgcga agcttctcgc
     7981 cctcgagacg tttttctagc acggttcccc cggcgtgaat gaacgccgtg ccgtcaccgg
     8041 ccagacgctg aaggataaag ccctcgccac cgaagaagcc cgcaccaaga cgcttgctga
     8101 acgccacgga cacggaggtg cccatctccg cgcagagaaa agcgtccttc tgacagacga
     8161 tctcgccacc tgcctgtgcc agatccaccg cgaccacctg cccgggatac ggagccgcaa
     8221 aacccatctg ccgcgggccg tcggcttcgt tggtgaagtg cgtcatgaac agcgactcgc
     8281 tgccgagcag ccgtttggcc gcaccgaaga gtttacccac caccccgctg gccgacccgt
     8341 ccccgagccg tgcctcgtag gcaatgccct cgctcagata catcatggtg cccgcttcgg
     8401 cgaccacaga ctccccggga tcgagttcta cttcgaggta ctgcatggat tcaccgcaaa
     8461 tccggtagtc gatctcgtgc gccatcgcgc ggtcctcctc gttctcaagc cccgacctgc
     8521 accttctggc ccccggcgac gccaaaaggt ctagttgaaa taacacccgc agcgcgataa
     8581 ttattgccat gtgaggggat ggctggcaaa gaaccggccg ccgagcaggg ggcatgacgt
     8641 cgtgaaaaca aagatcttcg atcggtttct caccaacggg acgctggcca tcgtcgacgc
     8701 cgatggttca agccgtcagt tcggctgcgg cgctccgttc gccacgatgc gcttgaaccg
     8761 gcgcgggacg ttgacccgga ttctgcgcaa tcccgagctc aacctgggcg agacctacct
     8821 ggcaggcgag tgggacgtca tcgagggctc tctgcacgat ctgctcacca ttttgcgcac
     8881 gaatctcgag cgtcacgccg gtcgatcgcg gcttgcccgg gcgctcgcgc tcatgctcgt
     8941 cccctggaac cacgtggccg ccagccaccg caacgtccgc catcactacg atctggacga
     9001 gacgctcttt cgcgcgtgcc tggacaccaa catgcactac tcgtgcgcgt actaccgcga
     9061 agcgaacatg agcctggagg aggcacagca ggccaagtgc gaccacatcg cggccaagct
     9121 ctgcctgcgc cccggcatgc gcgttctcga cgttggctgc ggctggggca gcctggcgat
     9181 ggaactcgcc gagcggcacg acgtaacggt gatgggggtt accctctcgc cggagcagct
     9241 gcgtgtggcc cggagcacgg cccgcctgcg gggcctcgac aatcacgtgc gcttcgagct
     9301 tcaggattac cggcaggtag aggagagctt tgacaggatc gtctccgtcg gcatgttcga
     9361 gcacgtcggc cgtcgccact acgacgagtt cttcggcaag gtcgaagcgc tgctcgcaga
     9421 cgacggcgtt gcgctgctgc acaccatcgg ggcgtccagc ccgcccagcg tggtgaaccc
     9481 gtggatcgag cgccacattt ttcccggcgg ctacatccct tcactctccg acgtggcgcc
     9541 agccgtggag cgcagcgggc tgatcaccgc cgacgtggag gtccttcgtc atcactacgc
     9601 gctgacgctc caggagtgga accgccggtt ccagcaaagg cgcgccgagt tcgctcagag
     9661 caaaggcgag gcgttctgcc gcatgtggga gttttacctt ctcgcgtgcc agaccgcctt
     9721 cgaggtgtcg gatctggtgg ttcaccactg gcagctggcc aagcgcaact tgagcgtgcc
     9781 caccacgcgc aactatctgt acgagacgca gccgtacgtg aagatccccg gggaaaccgc
     9841 ggccgaagag atcaccctca ggcagcatca gggctgatcg gccaggcagg caacggcgcc
     9901 aagcagaccc ggaaactcat cctcgatgac gtagagcggc agccgcgctg cgtagtcggc
     9961 caacacgccg cgttcctcga agcgcctgcg cagggggctg gactccgcga actcgacgag
    10021 cttgggcacg atgccgccgc cgatgaatac gccgccttcc gcacagacgg tgagcgcgag
    10081 gttccccgcc gcggcgccga gaaagccgaa gaacatttcc agcacctgat ggcagatcgg
    10141 gtcctccacg accagcccct gcctgctgat ctcctcggga gccagcgctt cctccggcac
    10201 tccccagatg gcgcatagcg cgcagtagag gttcaccagg ccgggtcctg acagcacgct
    10261 ctcccagctg acgtggggga gccttgcggc aagcacctgc agcacctcct gctccagggg
    10321 agagccgggc gcgaggtcgg cgtgcccgcc ctcggacgga agcaccaggg gcggaccttc
    10381 ccgggtcggc gccagcacgg ccatccccaa cccgctgccg ggtccaagca gcgccctgac
    10441 gccctcgtcg ggctcgcccg gtccgatctg acgcaggtgg cgcaggaacg ggatgccccg
    10501 agccacggcg tagaaatcgt tgaccacacg ggcgggacag ccgagctcgg cacccagcgc
    10561 gtcccccgaa acggccagcg acacgttcgt caggctgccc tgattggcgt gaacggggcc
    10621 cgccatggcg atgcagcacc cgtccaacgc ctggccgtcg aaagccgaac gcgcggcttc
    10681 ggcgagaccg gccacgttcc tgagcgaggc cgtctgcgaa atcagcacgg gggacgtgag
    10741 ccgcgttccg tcggttaacg ccaggcgggc attggtgcca ccgatgtccg cgaccagata
    10801 cgctccgtcc gccatcagcg aggcagcacc ttgagatcct gcagggcggc aagctggttg
    10861 cggaacatct gctcggtgtc tatgcattcg tggaagccga gctggcgaat cttgattgtg
    10921 ctcagcagcg tgctccgcgc cccgccgcca gagccgaata ccgcgtcggc gaactgccat
    10981 gaatcgccca ccagctcggc catggccatg gacttaagcc cgtgccgtcc gaccacctcg
    11041 tcccagacac cggagagttc cggcatgctg atcgcgagcc gcatggggtc cgcttcgccg
    11101 gtgggcattt gaaaaatctc accgaacgcg ggccacaggg agacccaatc gagcacgtcg
    11161 ccgttggtga cgttgaggat ctcgttggcg caggcgggtt cgcgcccggc ccagatgatc
    11221 gcgcgggcca gcaggtcggc gtccgtggcc tcggtaacga aaccgccgcc acccggaaag
    11281 caaagcggct tgccgagctg gcgcatcacg gcggcgtaaa caccgatggc cgccaccatg
    11341 ttcatggggc tgccgagcgc tacgccgcag acgatctgtg ggcggacgat gctgaaggcc
    11401 caggacgatc cggcctggcg gctgcgcatc aggtcttcct gctcccagta gaagttctcg
    11461 cccgggtgcc ggggcgccga ctccttcccc ggcaacgcca tgggcgcgag atgagcgccg
    11521 taggctttgg tcccctgcag cagcgtgaag tgctcccgtg gctgcacgaa ctccagcagg
    11581 ttggcgagca tccgcgcgtt ggtttcgatc tggacggggt cccgccagcc cgatatcaga
    11641 tccggcattt cctgaagagc ggcgtagacg atgtgggtaa cgccttgcgt ctgcgcggcg
    11701 cccaacgccg cgtggcacgc atcgcggtcc gtcagatcgc aggggatcca gcgcgcccgg
    11761 gtgggaaagt ccggctgccg acgggaaagc ccgatgacct ctgctcgatc ttcagcctcc
    11821 agagccgaca gcacggcgcg gccgacgagc ccccgggccc ccacgaccag atatctgcga
    11881 tcctccatgc cgccattgtc cgcatgccac ccatccggtt caattgccag gccaccaact
    11941 cggcccgcta taatccggtt cccccgaagc caggattaat tcatgacggt cagcgccgga
    12001 ctcggcatcg cgaattttcc gttccaggac gggccagact tctggcgctg ggtagatctg
    12061 tgcgacgccg ggggggtgga ttcaatctgg cagagcgatc ggatcatcag tcgcgacccg
    12121 cagctggaat gcatgagcgt catggccgcg ctggccggcg gcagccggcg cctgaagttc
    12181 ggcatgaacg tagcgagcct ggcgctgcgc gaccccgtgc tcaccgccaa ggcctgcgcc
    12241 accatcgacg tgctcagcga cgggcggctc ctccccgcgt tcggcgtggg cagcgctctg
    12301 tccagagatt tcaccgccac gggcacaccc acccgcggtc gcgggaaacg cacggcggag
    12361 ggtctggaaa tcatggccag gctatggtcc gaggaaagcg tgagcttcca tggggagttc
    12421 taccagctgg acgacgccag catctcgccc cggcccgttc agcagccgct gccgctctgg
    12481 gtggggggct ccgcgaaaca ggccattgaa cgaaccgccc gctggggcac cggctggcag
    12541 gccggtatcg agtccgcgga ggaggtcgcg cccgtcatcg ccgccatcaa ggcagaggcg
    12601 gcgcagctcg gccggtccat cgacgaggat cacttcggcg cgggtttcgg ttttcgtttc
    12661 ggctcaccgg acgagccgat ctgcacgcgg tatctggagc tgctgcgcaa gcgcctggga
    12721 cgcgaaccca agggctttct cgcggtgggc ggggtcccgg agatcatggg gctgctccac
    12781 gagtttcggg acgccggcgt gcacaaattc atcctgcgcc cggtagccac ggatggtgag
    12841 gacttcgtcg accagaccag gcgattcatc gagcacctgc tgccggaagt ctcggcgcta
    12901 aacggttagg tcggtttcct cgcccaccgc aatcggccgc gccgcgcccg ccttccgagc
    12961 ctagaaaatc ccagtggata acccaggtaa agcccgccac aatgagccgg cggggggaaa
    13021 tcaatggctg gaatactggc aggtctcggc agcaccaagg caagtggcgg ggccgggttc
    13081 tgcttaccgc cgagtgctct ccccatcaac caatcgggag atcaagaatg aaaaagatac
    13141 taggtgcaat gatggttgcc ctgtgcgctg cttccggagc gtatgcccag gagcagca
//
LOCUS       DBNHLM010000023         5337 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_421624, whole genome shotgun sequence.
ACCESSION   DBNHLM010000023 DBNHLM010000000
VERSION     DBNHLM010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_421624"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="AC16QF_01145"
     CDS             <1..226
                     /locus_tag="AC16QF_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HIR6447184.1"
                     /translation="VLTEAIDMGGTTLRDFVNQDGNPGYFKQSLNVYGRLGLPCHRCA
                     TPLKGTRTGQRATVFCPNCQGAQSFSGRNR"
     gene            289..600
                     /locus_tag="AC16QF_01150"
     CDS             289..600
                     /locus_tag="AC16QF_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447185.1"
                     /translation="MKRMKRAILAFTLALFMATPALAAPEDSPSAFTMTGDLVVARPL
                     GVAMTAIGAAVFVVSLPFSALGGNVGESADALVMGPVNETFVRCLGCPSAGRRQAPPA
                     N"
     gene            complement(649..1122)
                     /gene="coaD"
                     /locus_tag="AC16QF_01155"
     CDS             complement(649..1122)
                     /gene="coaD"
                     /locus_tag="AC16QF_01155"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR6447186.1"
                     /translation="MRTVVYPGSFNPITNGHTDLVKRALNLFDHVIVAIGTSAQKDPS
                     VALKNRIDLCRLVLAEFGDKVTVEGFNSLLVNYVRERNARFILRGLRTVTDFDYEFQM
                     AEMNQSLAPEIEYVFLPTAQNCSFISSTLVREIASLGGDISQFVHPAVVEAYHRD"
     gene            complement(1165..1752)
                     /gene="rsmD"
                     /locus_tag="AC16QF_01160"
     CDS             complement(1165..1752)
                     /gene="rsmD"
                     /locus_tag="AC16QF_01160"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR6447187.1"
                     /translation="MSDWHGSLTILRMASNQVRIIGGKWRGRKLAFPDAATLRPTLGR
                     VRETLFNWLAGRIAGACCLDLYAGTGALGFEALSRGAAEADMVERARTAVAALRRNAQ
                     LLKAPARVHGIPAERFLQATDRTWDVIFLDPPFADDALPRALEVIAERGLLRPGGCVY
                     FEQAERGGEEPAGPGWRLLKKSRAGASEFGLLVRD"
     gene            1793..2746
                     /gene="ftsY"
                     /locus_tag="AC16QF_01165"
     CDS             1793..2746
                     /gene="ftsY"
                     /locus_tag="AC16QF_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR6447188.1"
                     /translation="MSEQDQSSAEGGGLWSRLKRGLGQTRNQLAEGVGNLLLGEKEID
                     DEVLSELETALLLTDVGIDTTREIMDKLAVRVRRKELNDTVALHQALGSLLKELLLPV
                     EKPFTIAQDKRPFVIFMVGVNGAGKTTTIGKLAKRLRDDGLNVVLAAGDTFRAAAVEQ
                     LQTWGERIGVPVIAQGQGSDSASVVYDAVAAARARGHDVVLADTAGRLQAKANLMEEL
                     AKVKRVVNRMDPDAPHEVLLVLDAGVGQNALSQVREFDAAVGVTGLIFTKLDGTAKAG
                     VLFGIARQVMKPVYFVGVGEGADDLRPFDAKDFVDALLAPE"
     gene            2761..3453
                     /gene="ftsE"
                     /locus_tag="AC16QF_01170"
     CDS             2761..3453
                     /gene="ftsE"
                     /locus_tag="AC16QF_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR6447189.1"
                     /translation="MGQIEFHHVTKRFDDGFEALKDVSVDFPEGSMTFLTGHSGAGKS
                     TFLKLLLCADRPSAGQIIVNGVNVRHISARRLAHYRQHIGVVFQDHHLLHHRDVYENV
                     ALPLRVAGMRERAVARRVRASLTRVGLLAKERMFPRYLSTGEQQRVGIARAVATRPRI
                     LLADEPTGNLDPELSRDIMHVFEQVNQVGTTVIIASHDRDLIESMGLRIIELAHGNIV
                     RDTDREADIGFE"
     gene            3440..4456
                     /locus_tag="AC16QF_01175"
     CDS             3440..4456
                     /locus_tag="AC16QF_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HIR6447190.1"
                     /translation="MASNNEAQGGRPAPATGGGRGRTKKGYPWPHRRVGLVAWWQDHL
                     RTGRETLHFISARLGTSLLVWLLVGIALALPAGLILLQLNLAGMTADWDGRPGLSVYF
                     ELNTDAGKVAAIAQRLSERAEIEGVRVTSPEEALAEFQSHADVGDALDLLTENPLPAS
                     LRATLVPGSSLLDLELVAGLVEGAPGVSEVVVEKTWLERVIDISRVVSRLGIILGALF
                     GVGAVLVTATSVRLAIEARLEELRVLKLVGATDRQIRRPFMYFGALYGLGGGLVAAML
                     ISLCLVAIETPVSNLLGSYGRDLETPGFDPTFLSGLLLVGAFLGVSGALLAARQRLKD
                     LEIL"
     gene            4547..>5337
                     /gene="rpoH"
                     /locus_tag="AC16QF_01180"
     CDS             4547..>5337
                     /gene="rpoH"
                     /locus_tag="AC16QF_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009315054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HIR6447191.1"
                     /translation="MSTDVLSMKSLAPGRDLDAYVQTVSAFPVLSAEQEKDLAERFYY
                     DNDLEAARELVLCHLRFVVHLARSYRGYGLPQADLIQEGNVGLMKAVKRFNPEVGVRL
                     VSFAVHWIKAEMHEFILRNWRIVKVATTKAQRKLFFNLRSSKKRLAWLTQEETRAMAR
                     ELGVSEEEVARMEGRMAASDLAFDGYASDDDSSPLAAPVHYLEHEGSNPEDLVAEEDW
                     HDEASARLGAALAELDERSRAIVEARWLAEKKATLHELGDTYGVSA"
BASE COUNT          951 a         1618 c         1800 g          968 t
ORIGIN      
        1 ggtgctgacg gaagccatcg acatgggcgg caccacattg agagattttg tcaaccagga
       61 tggcaatccg ggttatttca agcagtcgct caacgtctat ggccggctcg gattgccctg
      121 ccatcgctgc gcgacgccgc tcaaagggac ccgcaccggg cagcgagcta ccgttttttg
      181 tccaaattgc caaggcgccc aaagttttag cggacgcaat cgctaaagta ggcgcggtgc
      241 gctcggcacc aggaaaaggg tcgagggcgg ttgactagca aaaaggacat gaagcgaatg
      301 aaacgcgcaa ttctggcatt caccctggcc ctgttcatgg ccactccggc acttgctgcg
      361 ccggaggact caccctctgc attcacgatg acgggcgatc ttgtcgtcgc caggcccctt
      421 ggcgtcgcca tgacggcaat cggcgccgca gtattcgtcg tcagcttgcc gttctcggca
      481 cttggcggaa acgtcggcga gtccgcggac gcgctggtca tgggccccgt caacgaaacg
      541 tttgtgcgtt gcctcggttg ccccagcgcc ggtcgccgcc aggcgccgcc cgccaactga
      601 tttccccctg atgccggggc tcttcgatag gggccccggt gctggtggct agtcccggtg
      661 gtaggcctcg accaccgcgg gatggacgaa ttgggagatg tctccgccca gggaggcgat
      721 ctcgcgcacc agcgtggacg aaatgaaaga gcagttctgc gctgtgggca gaaacacgta
      781 ctcgatctcc ggtgctagcg actggttcat ctccgccatc tgaaactcgt agtcgaagtc
      841 ggtcaccgtg cgcaggccgc gcagaatgaa ccgcgcgttg cgttcccgca cgtagttcac
      901 cagcagggaa ttgaatcctt ccacggtgac tttgtcgccg aactccgcca gcaccaagcg
      961 gcagagatcg atccggttct tcagcgccac tgaaggatct ttctgtgccg acgtgccgat
     1021 ggccacgatg acatggtcga agaggttgag cgcccgcttg accagatccg tgtgtccgtt
     1081 agtgatcggg ttgaacgaac ccgggtatac gaccgtgcgc attgtcccca ccccactgag
     1141 aagcccgcga tggtatcggg gcggtcaatc cctgacaagc aggccgaact cgctggcgcc
     1201 ggcgcggctc ttcttcagca ggcgccagcc agggccggcg ggctcctcgc cccccctttc
     1261 ggcctgctcg aagtaaacgc agccgccggg ccgaagcagg ccgcgctcgg cgatcacctc
     1321 tagggcgcgg ggcagcgcat cgtcggcgaa tggcggatcc aagaagatca cgtcccaggt
     1381 gcggtccgtg gcttgcagga aacgttcggc tggaatgccg tgaacgcggg ctggcgcctt
     1441 cagcagctgc gcgttgcgtc gcagcgcggc cacggcggta cgcgccctct ccaccatgtc
     1501 tgcctccgcg gcgccgcggg acagcgcttc gaaacccagc gcgccggtgc cggcgtagag
     1561 atcgaggcag caggcgccgg cgatccggcc cgcaagccag ttgaacagag tttcgcggac
     1621 gcgacccagg gtgggcctga gcgtggcggc atccgggaag gcgagctttc gtcccctcca
     1681 cttaccgccg ataatgcgca cctggttgct ggccatccga aggatggtga gcgaaccgtg
     1741 ccagtcagac aagccgtaga ccggcgagcg catcgccggg aaaaggatca tcgtgagcga
     1801 gcaggatcag tcgagtgcgg agggaggagg gctgtggagc cggctgaagc gggggctcgg
     1861 ccagacgcgc aaccagctgg ccgaaggcgt cggcaatctg cttctcggcg agaaggagat
     1921 cgacgacgaa gttctctcgg agctggagac cgcgttgctg ctcaccgacg tcggcatcga
     1981 caccacccgg gagatcatgg acaagctcgc cgtgcgcgtc cgccgcaagg agctcaacga
     2041 caccgtcgcc cttcaccagg cattggggag tctgctcaag gagcttttac tgcccgtcga
     2101 gaagcccttc accattgcac aggacaaacg tccgtttgtg attttcatgg tgggcgtcaa
     2161 cggcgcggga aagacgacga ccatcggcaa gctggccaaa cgacttcgcg acgacggcct
     2221 gaacgtggtg ctggccgcgg gcgacacctt tcgcgctgcg gccgtggagc agcttcagac
     2281 ctggggcgag cggattggcg tgccggtcat cgcccagggt caggggtccg acagcgcctc
     2341 ggtggtctat gacgccgtgg cggcggcccg ggctcgcggc cacgacgtcg tgctggcgga
     2401 cactgcgggg cgcctgcagg cgaaggccaa tctcatggag gaactcgcca aggtgaagcg
     2461 ggtcgtgaat cgcatggacc cggacgctcc ccacgaggtc ctgctcgtgc tcgatgccgg
     2521 cgtggggcag aacgccctca gccaggtccg cgagttcgac gcggccgttg gggtgacggg
     2581 gctgatcttc acgaagctgg acggaacggc caaggccggg gtgctgttcg ggatcgcgcg
     2641 tcaggtcatg aagcccgtct acttcgtcgg cgtgggcgag ggtgcggacg acctcaggcc
     2701 cttcgatgcc aaagatttcg tcgacgccct tctcgcgccc gagtaagcaa agcgggcacc
     2761 atgggacaga tcgagtttca tcacgtcacc aaacgattcg acgacggctt cgaagcgctc
     2821 aaggacgtca gcgtcgactt tcccgaaggc tccatgacct ttctgaccgg gcactccggg
     2881 gccggcaaga gcacgtttct gaagcttttg ctgtgtgccg atcgcccgtc ggctgggcag
     2941 atcatagtca acggagtgaa cgtccggcac atcagcgcca ggcgtctcgc tcactaccga
     3001 cagcacatcg gcgtggtttt ccaggatcat cacctgctgc atcatcgtga cgtgtacgag
     3061 aacgtagcgc tgccgttgag ggtcgccgga atgcgcgaga gggcagtggc tcggcgggtc
     3121 agggcgtcgc tgacccgggt cgggctgctg gcgaaggagc ggatgtttcc gcgttacctt
     3181 tccaccggtg agcagcagcg ggttggcatt gcccgggcgg tggccacacg gccgaggatc
     3241 cttctggcgg acgagcccac gggcaatctg gatcccgagc tctcccggga catcatgcac
     3301 gtcttcgagc aggtcaatca ggtcggcacc acggtgatca tcgcgagcca cgatcgagac
     3361 ctgatcgagt ccatggggct gcgcatcatt gagcttgccc acggcaacat cgtcagagat
     3421 acggacagag aagcggacat tggcttcgaa taatgaggcg caagggggtc ggcccgcgcc
     3481 ggccacgggc ggcgggcgag gccgcacgaa gaagggatat ccctggcccc accgccgcgt
     3541 ggggctggtg gcctggtggc aggaccatct gcgcacggga cgggagacgc tgcatttcat
     3601 cagcgcccgt ctgggaacga gcctgctggt ctggttgctg gtggggatcg cgctggctct
     3661 gcccgcgggg ctgattctgc ttcagctcaa cctcgccggc atgacggccg attgggatgg
     3721 gcgaccgggg ctgtctgtgt atttcgagct gaacaccgac gcaggaaagg ttgcggcgat
     3781 tgcccaacgg ctgtccgaac gtgccgagat cgagggtgtc agggtgacct cgcccgagga
     3841 agcgctggct gaatttcaaa gtcacgcgga cgttggagac gctctcgacc tgctgacgga
     3901 gaacccgttg ccggcctcgc tgcgcgccac cctggtgccg gggtcttcgc tgctcgatct
     3961 ggagctggtc gcggggcttg tcgagggagc gccgggagta tcggaggtgg tggtcgagaa
     4021 gacctggctg gagcgggtca tcgacatttc ccgggtggtc agccgcctgg ggatcattct
     4081 tggggcgctt ttcggtgtcg gcgcggtgct ggtcaccgca acttccgtgc ggctggccat
     4141 cgaggcgcgg ctggaggagc tcagagtgct gaaactggtt ggggccaccg accgacagat
     4201 ccgtcggccc tttatgtact tcggcgcgct ttacgggctt ggtggcggtc tcgtggccgc
     4261 catgctgatc agcctttgcc tggttgccat agagacaccg gtctcgaatc tcctcggaag
     4321 ctacggccgt gaccttgaaa cgccggggtt cgacccgacc ttcctttccg ggctgctgct
     4381 ggtcggcgct ttcctgggag tgtccggggc gcttctggct gcgcggcagc gtctgaagga
     4441 tctcgaaatc ctttagtttt cggcgggtta gggaaccatt cgcggggtta gcagtctcac
     4501 caccagagtg ctaaactgct gaaattagtg ttaccggagg tatcacatga gcaccgacgt
     4561 gctttctatg aaaagtctcg ccccgggacg cgatctggat gcttacgttc agacggtcag
     4621 cgcgtttccg gttctttctg cagaacagga gaaggatctg gcggagcggt tttactatga
     4681 caacgacctg gaagccgccc gggaactggt gctctgccat ctgcgcttcg tcgtccacct
     4741 ggcccgttcg tacagaggct acgggctgcc tcaggcggac ctgatccagg agggcaacgt
     4801 tggcctgatg aaggccgtca agcggttcaa tcccgaggtg ggcgttcgcc tggtctcttt
     4861 tgccgtgcat tggatcaagg cggaaatgca cgagttcatc ctgcgtaact ggcggatcgt
     4921 caaggttgct accaccaagg cccagcgaaa gctcttcttc aacctgcgca gctccaagaa
     4981 gcggctcgcc tggctcaccc aggaagagac ccgtgccatg gcccgggagc tgggcgtttc
     5041 cgaggaagag gtcgcgagga tggagggccg gatggcagcg tccgacctgg ccttcgacgg
     5101 ctatgcttcg gacgacgact cctcgcccct ggccgccccc gtccactatc tggaacacga
     5161 aggttcgaat cccgaggacc tggtggcaga ggaagactgg cacgacgaag ccagtgcgcg
     5221 tctgggcgcc gcgctggccg agctggatga gcgaagccgg gcaatcgtgg aagcccgatg
     5281 gctcgccgag aagaaggcga cgctccacga gttgggcgac acatacggcg tgtcggc
//
LOCUS       DBNHLM010000024         2107 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1139501, whole genome shotgun sequence.
ACCESSION   DBNHLM010000024 DBNHLM010000000
VERSION     DBNHLM010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1139501"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..365
                     /locus_tag="AC16QF_01185"
     CDS             84..365
                     /locus_tag="AC16QF_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6447192.1"
                     /translation="MSTIEEQTKELKDRQAIYDCLMRYCRGIDRLDVGILKTAYHPDG
                     TDDHGIFSGNAWEFAEFIGPFDAELGVRQQTHRVDNVLIEFTGPDTAFT"
     gene            365..490
                     /locus_tag="AC16QF_01190"
     CDS             365..490
                     /locus_tag="AC16QF_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447193.1"
                     /translation="MDWNINGASTAEWQHGLFGRLPHGRIDMQDTSYDLLGDFRG"
     gene            483..1187
                     /locus_tag="AC16QF_01195"
     CDS             483..1187
                     /locus_tag="AC16QF_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009401404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6447194.1"
                     /translation="MAEQKVCVVTGVGPGTGAAMVRRFHEGGYRVAMIARTAERLEAL
                     AGEMPNAHAYPCDMADPAQIESTLARVADELGPPSIAIHNAVGGAFGDFLSIEPAQLE
                     ANFQVNVMGLLHLGRAVAPAMIEAGEGAIIVTGNTSALRGKAGFAGFAPTKAAQRILA
                     ESMARSLGPQHVHVAYIVIDAVIDLAWTRRRFADQPDGFFIQPASIAENAWYLAHQDP
                     SAWTFLSELRPHGENW"
     gene            complement(1203..>2107)
                     /locus_tag="AC16QF_01200"
     CDS             complement(1203..>2107)
                     /locus_tag="AC16QF_01200"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6447195.1"
                     /translation="VSPTLRWIRDCSGYYHEDRSGRLDRHPDPDEEPTMSEAVLKIFS
                     YLPNPRVWKAQIAAELCGVAVDVVGDRPSALQNWLWDFEARPLDEAERTADSPHARES
                     RRGFSGLLFKTDAFLTAHPFGTVPAAFAPGGEPGIFESNSILRAVARAGAANRQLYGG
                     DGYQASRIDGFLDANLVFAREAQVYLLGMDQPQAHVHERMAAAYQFYLAGVESALARS
                     AFLAGDELSIADISFVCDFAQFLREGHYEDALEKAGLSLISGNGPEDYPRSFHHLLTL
                     SAEPAFAQHMETYLDWYRRRAASA"
BASE COUNT          355 a          684 c          726 g          342 t
ORIGIN      
        1 gcggagtggg aggaaggcat gtacggcgag ctgcgcgccc ggggcgtgcg gggcaaggaa
       61 gacccggtct acacgttcct gtcatgagca ccatcgaaga acagacaaag gagctcaagg
      121 accggcaggc catttacgac tgcctgatgc gctactgccg gggcatcgac cggctcgacg
      181 tgggcattct caagaccgcc taccatcccg acggcacgga cgaccacggt atcttctcgg
      241 gcaacgcctg ggagttcgcc gagttcatcg gcccattcga tgccgagctc ggcgtcaggc
      301 agcagaccca tcgggtggac aacgtgctga tcgagttcac ggggccggac accgccttta
      361 cgtgatggac tggaacatca acggtgcgtc caccgccgag tggcagcacg ggctcttcgg
      421 ccggctgcct cacgggcgca tcgacatgca ggatacgtcc tacgatctgc tgggagattt
      481 ccgtggctga gcagaaggtc tgcgtcgtca ccggggtggg gccgggcacc ggtgccgcca
      541 tggtgcggcg gtttcacgag ggcggctacc gggtggccat gatagcccgc acggccgagc
      601 gcctggaggc gctggcaggc gagatgccga acgctcacgc ctatccctgc gacatggcgg
      661 atcccgcgca gatcgaatcg actctcgccc gcgtcgcgga cgagctcggc ccgccttcga
      721 tcgccattca caacgccgtg ggcggcgctt tcggcgactt cctgagcatc gagccggctc
      781 agctcgaggc caacttccag gtgaacgtca tgggtctgct tcacctcggc cgggcggtgg
      841 cgcccgccat gatcgaggcg ggagaggggg ccatcatcgt cacgggcaac acgtccgccc
      901 tgcgcggcaa ggcgggcttc gccgggtttg cccccaccaa ggcggcccag cggatcctgg
      961 cggaatccat ggcccgctcc ctgggccccc agcacgtgca cgtggcctac atcgtcatcg
     1021 acgcggtcat cgatctggcc tggacccggc ggcggttcgc cgaccagccc gacggctttt
     1081 tcatccagcc cgcctccatc gccgagaacg cctggtacct ggcgcaccag gacccgtcgg
     1141 cgtggacgtt cctgtcagag ctcaggccac atggcgagaa ctggtagcgg cgcgaacggc
     1201 ccttacgcgc tcgcagcgcg ccttcgatac cagtccagat aggtttccat gtgctgcgcg
     1261 aacgccggct cggcgctgag cgtgagcagg tggtggaagc tgcggggata gtcttccggg
     1321 ccgttgccgc tgatcaacga aaggccggct ttttccagag catcctcgta gtgcccttct
     1381 ctcaagaact gggcgaagtc gcagacgaag gagatgtcgg caatgctcag ctcgtccccg
     1441 gccaggaagg cgctgcgcgc cagcgcgctc tccacgccgg cgagatagaa ctggtaagcg
     1501 gccgccatgc gctcgtggac gtgagcctgg ggctggtcca tgccgagcag gtaaacctgg
     1561 gcttccctgg cgaagaccag gttggcgtct aagaagccgt cgatgcgcga tgcttggtac
     1621 ccatcgccgc cgtacagctg ccggttggcg gcacccgccc gggccaccgc gcgcaggatg
     1681 ctgttggact cgaagatgcc gggctcgcct cccggcgcga acgcggcggg gacggtgccg
     1741 aagggatggg cggtgaggaa ggcgtcggtc ttgaagagaa ggccggagaa accccggcgg
     1801 ctttctctgg cgtgggggct gtccgccgtg cgctccgctt cgtccagcgg ccgtgcttcg
     1861 aagtcccaca gccagttctg cagagcactg ggccggtcgc ccaccacgtc cacggccacg
     1921 ccgcagagct cggcggcgat ctgggccttc cacacccggg gattgggcag gtaggagaag
     1981 atcttcagga cggcttcgga catcgtgggc tcctcgtcag ggtctgggtg ccgatcgagc
     2041 cggcctgacc ggtcctcatg gtagtatccg gagcaatcac ggatccagcg aagggtaggg
     2101 gagacag
//
LOCUS       DBNHLM010000025         3120 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1080184, whole genome shotgun sequence.
ACCESSION   DBNHLM010000025 DBNHLM010000000
VERSION     DBNHLM010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1080184"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="AC16QF_01205"
     CDS             complement(<1..786)
                     /locus_tag="AC16QF_01205"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR6447196.1"
                     /translation="MAQTVTLTRRWPAQVEAHLQSLYDLSVDTTDRPRTRDELAAALA
                     SADALCPTVTDRIDADLLGSAEVRTRIIASFGVGHEHIDLDAARARGIVVTNTPGVLT
                     DCTADLTLALLLGIARRIGEGEREVRGGSWTGWRPTHMMGTRVTGKTLGIVGLGRIGV
                     AVARRARLGFGMRVLFHNPTPPPPERIAAAGAEPCSTLVELLQRSDFVSLHCPATADT
                     HHLINAERLAQMPAHAYLINTARGTVVDSEALATALRERRIRGA"
     gene            866..1477
                     /locus_tag="AC16QF_01210"
     CDS             866..1477
                     /locus_tag="AC16QF_01210"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6447197.1"
                     /translation="MRLHESPSPNARRVHVFLKEKGIEAPPTVRVDIRGGENLSDAYR
                     KLNPFGRVPVLELDDGTCLAESVAICRYFEGLHPEPSLFGREPLEMAIIEMWNRRAEI
                     NFMLQATGAFRNLTGFFKDREKVSKEWGAIALGNAADALGRFDTALGTSRFLAGETFS
                     IADITFAVVLQFAQMVKLDLPFDLPNIERYLAEVGSRPSFQES"
     gene            1542..2318
                     /locus_tag="AC16QF_01215"
     CDS             1542..2318
                     /locus_tag="AC16QF_01215"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6447198.1"
                     /translation="MKLEGKVAAITGGTRGIGRGIAEAFLAEGANVVVNGRTPEKGQR
                     ALEEMGVGDAAHFIAGDVRVQADVEAFVDGTIERYGKIDILVNNAGGSSGFAPISELS
                     DEAWNEALDWMLNATFWGTRRALPDMLSRKWGRIINISSVEGLLINKTTVGHYIVNKH
                     AINALTKVVAYENGPDGITSNAILPGAVETDLMTITGAEAAKSLGMTYEQYIDGYAQE
                     AAIKRINTVEEVAAVALLLASDLGGGITGALMEVHGGTAL"
     gene            2351..>3120
                     /locus_tag="AC16QF_01220"
     CDS             2351..>3120
                     /locus_tag="AC16QF_01220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6447199.1"
                     /translation="MAAARSLPYPNRAYAWFVLAVLVVAALIAFIDRQVVAIVVDPMK
                     ADLGVGDAQIGWLYGVFALFYAVAALPIAWLADRKSRKHIIAAGIFLWSLMTIACGLS
                     RSYWNVFLARIGVGIGEATLTPATTSMVGDYFPRDQIPLALSIFQTGAIMGSGIAFII
                     GGVVLNIVEQAEPMVLPLVGELRPWQQTFVYVGAPGMILALLLLLLREPVRRSAGGTG
                     TQQEATFAEIVAFYRRNARTLACHHIGFLSFALMGYAFV"
BASE COUNT          519 a          985 c         1061 g          555 t
ORIGIN      
        1 ggcgccgcgg attcggcgct cgcgcagcgc cgtcgccagg gcttcggaat ccaccaccgt
       61 gcctcgggcc gtgttgatga gataggcgtg ggccggcatc tgtgcaagcc gctcagcgtt
      121 gatcagatgg tgggtgtcgg cggtggctgg gcagtgcagc gacacgaagt cggagcgttg
      181 cagcaactcg acaagggtgg agcatggctc ggcgccggca gccgcgattc gttccggtgg
      241 tggcggcgtc gggttgtgga agagcacgcg catcccgaaa cccagccggg cgcgccgggc
      301 cacggctacg ccgattctgc cgagcccgac gatgcccagc gtcttgcccg tcacccgcgt
      361 gcccatcatg tgtgtcggac gccagcccgt ccaggacccg cctcgaacct cccgttcgcc
      421 ctcgccgata cggcgggcga taccgagcag cagcgccagg gtgagatcgg ccgtgcagtc
      481 ggtcagcact ccgggcgtgt tggtgaccac gatcccccgg gcccgggcgg catccaggtc
      541 tatgtgctcg tgaccgacgc cgaagctcgc gatgattctc gttcgcacct cagctgagcc
      601 cagcaggtcg gcatcgatcc ggtccgtcac ggtggggcag agcgcgtccg cgctagcaag
      661 cgcggcagcc agctcgtcgc gggtgcgcgg tcggtcggta gtgtctaccg agaggtcgta
      721 aagcgactgg agatgggcct ccacctgcgc aggccagcgc cgggtgagcg tgactgtctg
      781 ggccatatgc tttgtcctcg gaagcgaatc atggtattgc acatcgcgta caattgtccg
      841 catcgctgaa gcggggagag ttctgatgag gctgcacgag tcgccatcgc caaatgcgcg
      901 ccgggtccac gttttcctga aagagaaggg catcgaagcg ccgcccacgg tgagggtgga
      961 catccggggg ggcgaaaatc tcagcgacgc ctaccgcaag ctcaacccct tcggccgggt
     1021 tcccgtgctg gagctggatg acggcacctg cctggcggaa tccgttgcca tctgtcgcta
     1081 cttcgagggg ttgcatccgg agccctcgct gttcggccgg gagcctctgg agatggcgat
     1141 catcgagatg tggaaccgcc gggccgagat caacttcatg cttcaggcaa ccggcgcgtt
     1201 ccgaaacctc accggcttct tcaaggaccg tgagaaggtc tccaaggaat ggggcgccat
     1261 cgcgctgggc aacgccgcag atgcgctcgg gcgcttcgac accgcgctcg gcacttcccg
     1321 gttcctggcc ggcgagacct tcagcatcgc cgacatcacc tttgcggtgg tgctgcagtt
     1381 tgcccagatg gtcaagctgg acctgccttt tgatctgccg aacatcgagc gatatctggc
     1441 agaggtggga tcgcgaccca gcttccagga gagctgatct cagcgcgtcg ccctagcggc
     1501 ggccagaggg gtatggtcaa acgacgggag gaaaccgacg gatgaaactg gaaggcaaag
     1561 tggccgccat cactggcggg acgaggggca tcggccgcgg catcgcggag gcctttctgg
     1621 cggagggggc gaatgtcgtc gtgaatgggc gaacgcccga aaagggccag cgtgccctgg
     1681 aggagatggg agtcggagac gccgcgcact tcatcgccgg cgacgttcgc gttcaggccg
     1741 atgtggaagc cttcgtggac ggcaccatcg agcgctacgg caagatcgac attctggtga
     1801 acaacgccgg cggctcatcc ggctttgcac cgatttccga gctcagcgac gaggcgtgga
     1861 acgaagcgct ggactggatg ctcaacgcga ctttctgggg cacgcgacgg gcgctgcccg
     1921 acatgctgtc ccgcaagtgg ggccggatca tcaacatctc ctccgtggag gggctgctca
     1981 tcaacaagac gacggtgggc cactacatcg tcaacaagca cgccatcaac gcgctcacca
     2041 aggtggtggc ctacgagaac ggtcccgacg ggatcacctc caacgccatc ctgccgggcg
     2101 ctgtggaaac cgacctgatg accatcaccg gtgccgaagc cgccaagtcc ctcggcatga
     2161 cctacgagca gtacatcgac ggctacgccc aggaggcggc catcaagcgg atcaataccg
     2221 tggaggaagt cgcggcggtg gcccttctgc tggcctccga cctgggcggc ggcatcaccg
     2281 gtgcgctcat ggaagtgcat gggggcaccg cgctctgagc acgccctcgg gagccgggga
     2341 acgcggcgca ttggccgccg cgcgttctct gccgtatccg aaccgggcct acgcgtggtt
     2401 cgtgctggcc gttctggtgg tcgccgcgct cattgccttc atcgaccggc aggtggtcgc
     2461 catcgtggtg gatcccatga aggcggacct gggcgtggga gacgcccaga tcggttggct
     2521 gtacggcgtt ttcgcgctct tctacgcggt tgccgcgctc cccatcgcct ggctggccga
     2581 ccgcaagagc cgcaagcaca tcatcgcggc cggcatcttt ctttggtccc tgatgaccat
     2641 tgcctgcggg ctgtcgcgca gctactggaa cgtgttcctg gcccgcatcg gggtgggcat
     2701 cggcgaggcg acactcactc cggccaccac ctcgatggtc ggcgactact ttccgcggga
     2761 tcagattccc ctggcgctca gcatctttca gacaggggcg atcatgggat cggggattgc
     2821 cttcatcatc ggcggcgtgg tcctgaacat cgtcgagcag gcggagccca tggtgctccc
     2881 tttggtcgga gagctgcgcc cttggcagca gacgttcgtt tacgtggggg cgccgggcat
     2941 gatcctggct ttgctgttgc tgctgctcag ggagccggtg cgccgttcgg cgggaggcac
     3001 cgggacccag caggaagcga cctttgccga gatcgtcgcc ttctaccggc gcaatgctcg
     3061 cacgctcgcg tgtcatcaca tcgggttcct gtcgttcgcg ctgatgggct acgcgttcgt
//
LOCUS       DBNHLM010000026         7485 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_2797, whole genome shotgun sequence.
ACCESSION   DBNHLM010000026 DBNHLM010000000
VERSION     DBNHLM010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7485)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7485
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_2797"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="AC16QF_01225"
     CDS             <1..497
                     /locus_tag="AC16QF_01225"
                     /inference="COORDINATES: protein motif:HMM:NF016236.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HIR6447200.1"
                     /translation="ELDSALNGYLQLKPGSGTTDLARVALNTTVGIGGFFDVAATGGL
                     IYQEEDLGQTLAVAGITRTRYVYVPLAGPTTIRDAPYSFIRAWWPRLLLGSAYGWWMG
                     IIDLLNTRAEVLAVTEARDASALDPYAFTREAYYQRRKFLIYDGDPPMDDFFDDFEDE
                     DEAF"
     gene            499..3063
                     /locus_tag="AC16QF_01230"
     CDS             499..3063
                     /locus_tag="AC16QF_01230"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR6447201.1"
                     /translation="MAGAWLATPLSRWVGGVSRRPWTVLVCIAVATAALGWVALDRFR
                     MNSNLSDLIRQEATWRVDFDRFKEQFPDLVETAVVVVSGTSYRDVEAMTGRLEAALLG
                     RDDLFRAVYAPENDPFFRRHALLYLDEDDLDDMADRLAEAQPMLTAVAEDPSLRGVLE
                     LVGDGIENEPAAGFDTVVRLLAESGEAVLAGGDPTVSWTDELFETDGTIHRLIFLKGQ
                     ADFQESLANARIMKELRALIAQLPAIDGVDVRITGEVALAHEEIEAAYAGVQIAGWVA
                     LALLMIILIVGVRSAKIIIATVLLLFVGVTWTSAFAMLTVGEYNTLSIVFLVMFFGLG
                     VDFALHFSLRYQEEINSGEGDALRSLVSATNSVGGAIAICTITTALGFLSFYPTPYKG
                     LADLGVISAGGMLVAGFLTFTLLPALYAVLGDIRPHIVDLPTGDRLVAYLSRHRGPVL
                     AALGLGAALALFLASRAHFDYSVLALKDPRAESVVTLRVLQADDAVTDYALTIMSARQ
                     APEAALEELPTVETVLSPADYVPDGQDDKLFVLEDLQELLWSALEPLRTADPPTGTEL
                     REAAEALTATVAAAQAAAPSSPDVDRLAAVLTGLLERPPQALLIWQRGVITNLLEELG
                     WLREALYVDALAFEDLPEKLRARLVSEEGQRVTVVTPSEDVTAVEALSDFVSSVREVA
                     PTATGRPVIEWGVGDIVVGSFKQALGFATFAILAVLFVMFRNLRDSVLILVPLALAAL
                     FTLAASVVMDMPLNMANILVVPLIFGLGVDNGIHVVDRFHRAGDVGHLMHSSTPRAVM
                     LSTLTTIGTFAALMLSPHQGTASIGLLLTLAVGLLLLLTIFVLPVLLSLLVEPAET"
     gene            3060..4853
                     /locus_tag="AC16QF_01235"
     CDS             3060..4853
                     /locus_tag="AC16QF_01235"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIR6447202.1"
                     /translation="MSGVLLRWACLVVLLGFGVRVAIAVPLWSDPYVPGTEILLAVLV
                     GVYQDLIALVLLGGPLALAFLAGVRVGRAVLLLLVAVVLVVAFAELFFWWEFESRLNR
                     LVFHYLLFPREVVTFLEDQFYVSLYAIPALLIIAAAYLVVRGRSADQRAPSRWFLVGS
                     VLACVVAVLVASPLEFSSSRRINQMASNGYLGVLHAAVTDVSAWHGHYWNPAQPAPTR
                     GEAPGALGVVEPVWQPLPVKHVVLIVEESFSGEVWDDLALRGTYMPRFSSLADSGVLF
                     ENVYSTGTRTTRGLEALLNGFPPLPGIAVNQRGGFERLPSLARALSLAGFHNVFVYGG
                     WPGFSNFFPYWRALGYEELTSRDDFPEPRFETSWGVADEHLFEAIREHMSRLTQNHER
                     VFLTSLTVSHHRPFDFPAGRVPFPAEERRSEYALAYADWALGQFIEDASGEPWFDDTL
                     FVIAADHGPRISGDAPIPVAGFRVPLLLYAPAHLNPRRVDHLGSLMSFGVTLLELLGL
                     PNDEGLYGDNLLLTEQGLVPMEHEYQVGLLTGDGLTVLHLGGEVSGWRQEDDRLVLDA
                     PDWEVAARAARLFHGAHQWFYGSASAAADGR"
     gene            complement(4828..>7485)
                     /locus_tag="AC16QF_01240"
     CDS             complement(4828..>7485)
                     /locus_tag="AC16QF_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HIR6447203.1"
                     /translation="VELNDRERFITALKNSRKKGEVAELSGLHLLRPDRDSDFPVAAR
                     IAGLRTPDGVGGAIVTCRLMDDQIASERELRSSEARFSRIFHSSPDAILIVRNADSTI
                     LDFNAGFIRLLGYTREDAIGQAESDMQLWVHEAERERILNNLQTDQEVTAQETKLRRK
                     NGAVVYVELSLRYIEIEGELCILWIGRDVSERAIAEIARRQSEEKFVEVFNRSPDGIA
                     IVRQEDMLICDINEAFVNSSGHQREDLIGESLFELDVVSESALADAALRLEEDDHFSN
                     LEMTLHTKSGEEIPALVSATTLEVEEEPCFLIIAKDLRDLRATEEQLRASEQRFRGAF
                     ENAPIGILLVDMDGKVFQANRFAAELLSYRNASMRNLHISRLVPDDDRDKYNDLMQRL
                     ARGHQATLRTEQRMLCADGAEIWTNMHVVLLRSQDGEPAYGIVQIADITEMKHSQQRM
                     ERMAFYDILTDLANRRLFYDRLSQAIEYTTRNNRLAALLYLDLDQFKRVNDTLGHEAG
                     DTLLRTVAERLSHNVRQEDTVARPGGDEFTVLLYNIGKPEDAGHVAEKILNELRRPIT
                     VAGEQLVMTTSIGISIIPSDGTDPKQLMSNADLAMYRAKEQGRNQFQFYSKEMNTNAA
                     HRLKTENELRAALERQEFELYYQPKVRLSDLQIVGVECLLRWHHPERGLLLPGEFIEV
                     AEETGTIVDLGKWVIETACSAATQLAHEAGEPICTAVNISPRQFRDVELVSTVQRCLA
                     QTGLDPDNLEIEITETMLMDDVEAASATVCDLHDLGVRLAIDDFGTGYSSLNYLKKFP
                     IDTIKVDRSFVADIPESQDDMAITAAVIAMARRLHLAVVAEGVQTREQLEFLQQHECE
                     FAQGFLFSQAVPLEALKQLLVPNVRLLRR"
BASE COUNT         1105 a         2413 c         2459 g         1508 t
ORIGIN      
        1 gggagctgga cagcgcgctg aacggctatc ttcagctcaa gcccggatcg ggcaccaccg
       61 atctggccag ggtggctctg aataccaccg tcggcatcgg cgggttcttc gacgtcgccg
      121 cgacaggcgg cctgatctat caggaggaag atctggggca gacgctggcc gttgccggca
      181 tcacccgaac acgttacgtc tacgtgcctc tcgccgggcc gaccacgatt cgggacgcgc
      241 cttattcctt catccgggcc tggtggccgc gcctgctgct cggcagcgcc tacggctggt
      301 ggatgggcat catcgacctg ctcaataccc gtgcggaggt gctggccgtg accgaagcga
      361 gagacgcctc ggcgctcgat ccctacgcct ttacccgtga agcctactat cagcgccgca
      421 agttcctgat ttacgacggc gatccgccca tggacgactt cttcgacgac ttcgaagacg
      481 aagacgaagc cttctagaat ggccggcgcc tggttggcaa cgccgctgtc gcgctgggtg
      541 ggcggggttt cgcgccggcc ctggacggtg ctggtctgca tcgctgtggc taccgccgcg
      601 cttggctggg ttgccctcga tcgattccgg atgaactcga acctgagcga ccttattcgc
      661 caggaagcca cgtggcgggt ggatttcgac cgcttcaagg agcagttccc cgacctcgtc
      721 gaaaccgccg ttgtggtggt ttcgggaacc tcgtatcggg acgtcgaggc gatgaccggg
      781 cggctggagg ctgcactgct cggccgggac gatctttttc gcgccgtgta cgcgccggag
      841 aacgatccgt tcttccgccg ccacgcgctg ttgtacctcg acgaggacga tctcgacgac
      901 atggccgacc ggctggcgga ggctcagccg atgctcaccg ccgtggccga ggatccgagc
      961 ctgcggggcg tgctcgagct cgtcggcgac gggatcgaga acgagcccgc ggccgggttc
     1021 gacacggtgg tccgtctgct cgccgaatcg ggcgaggcgg tcctcgcggg aggggatccg
     1081 acggtctcct ggacggacga gctcttcgag accgacggga cgatccaccg gctgatcttt
     1141 ctcaaaggtc aggcggattt tcaagagtct ctggccaacg cccgtatcat gaaggagctc
     1201 agagccctca ttgcccagct gccggccatc gacggggtcg acgtgcggat caccggcgag
     1261 gtggctcttg ctcacgagga gatcgaggcc gcctacgccg gcgtccagat cgccgggtgg
     1321 gtggcgctgg ccctgctgat gatcattctg atcgtcggcg tgcgctcggc gaagatcatc
     1381 attgccacgg tgctcctgct cttcgtcggc gtgacctgga cctcggcgtt cgcaatgctc
     1441 acggtgggcg aatacaacac gctctccatc gtcttcctcg tcatgttctt cggcctgggc
     1501 gtcgacttcg cgctgcattt ctcgctgcgt taccaggaag aaatcaattc gggagagggc
     1561 gatgccctgc gctcgctggt gtcggccacg aacagcgtcg gtggagccat cgccatctgc
     1621 accatcacca ccgcgctggg gttcttgagc ttctatccca cgccgtacaa gggcctggcc
     1681 gacctgggcg tgatctccgc gggcggcatg ctcgtggccg ggtttcttac cttcaccctg
     1741 ctgccggcac tttacgccgt gctcggggac atcaggcccc atatcgtcga cctgcccacg
     1801 ggtgaccgcc tggtcgccta cctctcgcga catcgaggac ccgtgctcgc ggcgctcggc
     1861 ttgggcgcag ccctcgctct ctttctcgcg tcccgcgcgc acttcgacta cagcgttctc
     1921 gcgctgaagg accccagggc ggagtccgtc gtgacgctgc gcgtcctgca ggcggacgac
     1981 gctgtgacgg actacgcgct caccatcatg tccgcgcggc aggcgcccga ggcggcgctg
     2041 gaagaactgc cgacggtgga gaccgtgctg tcaccggcgg attacgttcc cgacgggcag
     2101 gacgacaagc tgttcgtcct ggaggatctg caggagttgc tatggagcgc gctcgagccg
     2161 cttagaaccg ccgatccgcc cacgggaact gaactgcgtg aggccgcgga ggccctgacc
     2221 gcgacggtgg ccgcagccca ggccgccgcg ccgtcctcgc cggacgtgga ccggttggcc
     2281 gccgtgctga ccgggctcct cgaacggccg ccccaggcgc tgctgatctg gcagcggggc
     2341 gtcattacca accttttgga agagcttggt tggcttcgtg aagccctgta cgtggatgcg
     2401 ctcgccttcg aggacctgcc ggagaagctg cgcgcccgcc tggtcagcga ggagggacag
     2461 cgggtcaccg tggtgacgcc ttcggaggat gtcaccgccg tcgaggcgct gagcgacttc
     2521 gtctccagcg tgcgcgaggt cgcgcctacc gccaccggac ggcccgtcat cgagtggggc
     2581 gtcggcgaca tcgtggtcgg ctcgttcaag caggcgctcg gctttgccac cttcgccatc
     2641 ctggccgtgc tgttcgtcat gttccgcaac ctgcgcgaca gcgtgctcat cctcgtgccc
     2701 ctggcgcttg ccgcgctgtt taccctggcg gcaagcgtcg tgatggacat gcccctgaac
     2761 atggccaaca ttctggtggt gccgctcatc ttcgggcttg gcgtggacaa cggcatacac
     2821 gtggtcgacc gatttcatcg cgcgggcgat gtcggccacc tgatgcactc gagcacgccc
     2881 cgggccgtca tgctttcgac gctgaccacc atcgggactt tcgcggccct catgctctcg
     2941 ccgcaccagg gaacggcatc catcgggctg ctgctgaccc tcgcggtcgg ccttcttctg
     3001 cttctgacca tattcgttct gccggtgctt ctgtctctgc tcgtcgagcc cgccgagaca
     3061 tgagtggagt gcttctgcgc tgggcctgcc tggtggtcct gctcggtttc ggcgtcaggg
     3121 tggccattgc ggtcccgctg tggagcgatc cctacgtgcc gggcaccgag atacttctcg
     3181 ccgttctggt cggcgtttac caggatctga tcgcgctggt tctgctcggc ggccctctgg
     3241 cgctagcctt tctggcgggc gtccgcgtgg ggcgtgcggt tctcctgctc ctcgtggccg
     3301 tggtgctcgt ggttgcgttt gccgagttgt ttttctggtg ggagttcgaa agccggctca
     3361 atcgcctcgt cttccactat ctgctgtttc cccgggaggt ggtgaccttt ctagaggatc
     3421 agttctacgt gagcctgtac gccattcctg cgctgctcat catcgcagcg gcctacctcg
     3481 tcgtgcgcgg gcgttcggcg gaccagcgcg cgccttcccg ctggtttctc gtcggctcgg
     3541 tgctcgcctg cgtggtggcg gtgctggtgg cctcgccgct ggagttcagc tcgtcacggc
     3601 gcatcaacca gatggcgagc aatggttacc tgggcgtgct ccacgcagcc gtgaccgacg
     3661 tttctgcctg gcacggtcac tactggaacc cggcgcagcc cgccccgaca cgtggggaag
     3721 cacctggcgc tctgggggtg gtggagccgg tctggcagcc gctcccggtt aagcatgtag
     3781 tgctgatcgt ggaggaatcc ttctccggcg aggtttggga cgatttggcg cttcgtggga
     3841 cctacatgcc acgtttctct tcactcgccg attccggcgt gcttttcgag aacgtctact
     3901 cgacgggaac ccgcacgacc cgggggttgg aggccctgct gaacggcttt ccgcccctgc
     3961 cgggcatcgc cgtcaatcag cgcggcgggt tcgaacggct gccctccttg gcgcgggcat
     4021 tgagccttgc cgggtttcac aacgtcttcg tttacggcgg atggcccgga ttctcgaact
     4081 ttttccccta ctggcgtgcc ctgggatacg aagagctgac cagccgcgac gacttcccgg
     4141 agccgcgttt cgaaaccagc tggggcgtgg ccgacgagca tctgttcgaa gccatccgtg
     4201 agcacatgag ccgtcttacc cagaaccacg agcgggtgtt cctgacgtcg ctgacggtca
     4261 gccatcatcg cccgttcgac tttccggcag ggcgggtccc gtttccggcc gaggaacgcc
     4321 gctcggagta cgcgctggcc tatgcggact gggccctggg ccagttcatt gaagatgcga
     4381 gcggggaacc ctggttcgac gacaccctgt tcgtcatcgc tgccgaccac ggcccccgca
     4441 tcagcggcga cgcgcccatt ccggtcgcgg gttttcgcgt tccgctgctg ctgtacgcgc
     4501 cagctcacct gaacccgcgt cgggtcgacc atctggggtc gctgatgagt ttcggcgtca
     4561 cgctgctcga gctgctgggg cttccgaacg acgaaggcct ctacggagac aatcttctgc
     4621 tcaccgaaca gggcctcgtg cccatggagc atgaatacca ggtgggtctg ctcaccgggg
     4681 atggcctgac cgtgttgcac cttggcggtg aggtctcggg gtggcggcag gaagacgacc
     4741 ggctcgtgct cgatgcgcct gattgggaag tggcggcacg ggcggcgcgc ctcttccacg
     4801 gcgcccatca gtggttctac ggtagcgcta gcgccgcagc agacggacgt taggcaccag
     4861 cagctgtttc aaagcttcca gcgggacggc ctggctgaaa agaaacccct gggcgaattc
     4921 gcactcgtgc tgctgcagga actcgagctg ctcgcgggtc tgcacgcctt cggcgaccac
     4981 ggccaggtgc agccgtcgcg ccatggcgat cacggccgcg gtgatggcca tgtcatcctg
     5041 gctctcgggg atgtcggcca cgaagctgcg atcgaccttg atggtgtcga tgggaaattt
     5101 cttcaggtag ttcagggaag aatacccggt accgaaatcg tcgatggcca gccgcacgcc
     5161 caggtcgtgg aggtcgcaca ccgtggccga tgccgcctcc acgtcgtcca tcagcatggt
     5221 ttccgtgatc tcgatctcca gattgtcggg gtccaggcct gtctgcgcca ggcagcgctg
     5281 cacggtgctc accagctcga cgtcccggaa ctggcgcggc gagatgttga ccgccgtgca
     5341 gatcggctcg cctgcctcat gagccagctg ggtggcggcg gagcaggccg tctcgatgac
     5401 ccacttgccg agatcgacga tcgtgccggt ttcctcggcg acctcgatga actctcccgg
     5461 cagcagcagg ccgcgctcgg ggtgatgcca gcgcagcagg cactccacgc ccacgatctg
     5521 cagatcggac aggcgcacct tgggctggta gtagagctcg aactcctgcc gctcgagagc
     5581 ggcccgcagc tcgttctcgg tcttcagacg gtgggcggcg ttggtgttca tctccttgct
     5641 gtagaactgg aactgattgc gcccctgttc ctttgcccgg tacatcgcga ggtcggcgtt
     5701 ggacatgagc tgcttgggat ccgtgccgtc gctggggatg atcgatatgc cgatgctggt
     5761 ggtcatcacc agctgctccc cggccaccgt gatcggtcgt ctcagctcgt tgagaatctt
     5821 ctcggcgacg tgccccgcgt cttccggctt gccgatgttg tagagcagaa cggtgaactc
     5881 atcgcccccg ggcctggcga ccgtgtcctc ctggcgcacg ttgtggctca gccgctcggc
     5941 caccgttcgc aggagggtgt ctcccgcctc gtggccgagg gtgtcgttca cccgcttgaa
     6001 ctggtcgagg tccaggtaca gcagagcggc aaggcggttg ttgcgcgttg tgtactcgat
     6061 ggcctgggag agccggtcat agaacagacg ccggttggcg aggtcggtga ggatgtcgta
     6121 gaaggccatg cgctccatgc gctgctggct gtgcttcatc tccgttatgt cggcaatctg
     6181 cacgatgccg taagccggct ccccgtcctg agatcgcagg agcaccacgt gcatgttggt
     6241 ccagatctct gcgccgtccg cacagagcat gcgctgctcg gtgcgcagcg tggcctgatg
     6301 ccctctggcg agccgctgca tcaggtcgtt gtacttgtcg cgatcatcat cgggaaccag
     6361 acgcgagatg tgcaggttcc tcatggaggc gttgcgatac gacaggagct cagccgcgaa
     6421 ccggttcgcc tgaaagacct tgccgtccat gtcgaccagc agaatgccga tgggcgcgtt
     6481 ctcgaaagca ccgcggaagc gctgctcact ggccctcagc tgttcctcgg tagcgcgcag
     6541 atcgcgaagg tccttggcaa tgatgaggaa gcagggttcc tcttcgacct cgagcgtggt
     6601 ggccgacacc agggcgggaa tctcctcgcc ggacttggtg tgaagggtca tctccaggtt
     6661 ggagaagtgg tcgtcctcct ccaggcgcag ggcggcatct gcgagggccg actcgctcac
     6721 cacgtccagc tcgaaaaggg actccccgat cagatcctcg cgctggtggc ctgaggaatt
     6781 cacgaacgct tcgttgatgt cgcagatcag catgtcttcc tggcggacga tggcgatgcc
     6841 gtccgggctg cggttgaaga cctcgacgaa tttctcctcg ctctgccgtc gggcgatctc
     6901 ggcgatggcc cgctcggaca cgtcccgacc gatccacagg atgcacagct ccccctcgat
     6961 ctcgatgtag cgcagggaca gctccacgta caccaccgct ccgttcttgc ggcgcagctt
     7021 cgtctcctgg gcggttactt cctggtcggt ctggaggttg ttgagaatgc gttcccgctc
     7081 cgcctcgtgc acccacagct gcatgtccga ttccgcctgg ccgatggcgt cttcgcgcgt
     7141 gtagccgagc aggcggatga agcccgcgtt gaaatcgagg atggtggagt ccgcgttgcg
     7201 gacgatgagg atggcgtccg ggctcgagtg aaagattcgg gagaaccggg cttcggagct
     7261 gcgcagctcg cgctccgatg cgatctggtc gtccatcagc cggcaggtca cgatggctcc
     7321 gccaacgccg tcgggcgtgc gcaggccggc gatccgcgcc gccaccggga agtcgctgtc
     7381 gcggtcgggg cgcagcaggt gaagaccgga gagctctgcc acctcgccct tcttgcggga
     7441 gttcttcagg gcggtgatga atcgctcgcg gtcgttcagc tcgac
//
LOCUS       DBNHLM010000027        11087 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_840941, whole genome shotgun sequence.
ACCESSION   DBNHLM010000027 DBNHLM010000000
VERSION     DBNHLM010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11087)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11087
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_840941"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="AC16QF_01245"
     CDS             complement(<1..393)
                     /locus_tag="AC16QF_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR6447204.1"
                     /translation="MTENHKILVLPGDGIGAEIVPVAQAVMGAAALKFGVSVAFEEGL
                     VGGIAIDETGSPLPDETLRQAREARAVLLGAVGGPRWDDLPTLQRPERGLLGLRAGLE
                     LFANLRPAIQFGPLAEASSLRAELVAGLD"
     gene            complement(413..1063)
                     /gene="leuD"
                     /locus_tag="AC16QF_01250"
     CDS             complement(413..1063)
                     /gene="leuD"
                     /locus_tag="AC16QF_01250"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010794947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR6447205.1"
                     /translation="MKAFDKVTGIVVPLDRANVDTDQIIPKQFLKSIHRTGFGDNLFD
                     AWRFEDEGDIGITPNERRINRAFVLNDPRYQGGSILLARANFGCGSSREHAVWALLEY
                     GFRCVIAPSFADIFLSNCFKNGLLPIVLEEEIIEELFVRSAGEEPLELTVDLPGQEVI
                     AGMGERYEFAIDANRKRALVEGLDDIGMTLANVDAIRAFEERHRRAMPWLFESHPT"
     gene            complement(1060..2397)
                     /gene="leuC"
                     /locus_tag="AC16QF_01255"
     CDS             complement(1060..2397)
                     /gene="leuC"
                     /locus_tag="AC16QF_01255"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR6447206.1"
                     /translation="MAAKTLYDKIWDAHVVGVREDGQTMIYIDRHLLHEVTSPQAFEG
                     LALAGRKPWRLDANLATPDHNVPTDRRELGIEGITDRRQGIVHVVGPEQGATLPGMTI
                     VCGDSHTSTHGAFAALAQGIGTSDVEHVLATQCLVQPKALNFRVAAEGELGVGVYAKD
                     LVLAIIGRIGTAGATGHTIEYQGSAIRSLTMEGRMTLCNMSIEAGARAGLVAVDDRTL
                     EYLRERPFAPVGAAWDAAEAYWRGLVTDSQAHFDRDLELNASEIEPQVTWGTSPEMVC
                     GISASTPNPDSVDDPQRSDGLRRALEYMGLGSDQPMTEIAVDRVFIGSCTNSRIEDLR
                     AAARVVKGRRVAGSLKQALVVPGSGLVKAQAEAEGLADVFLAAGFEWRAPGCSMCLAM
                     NPDRLLPGEHCASTSNRNFEGRQGHGGRTHLVSPAMAAAAGIAGRFVDVREYL"
     gene            2501..3421
                     /locus_tag="AC16QF_01260"
     CDS             2501..3421
                     /locus_tag="AC16QF_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008056358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6447207.1"
                     /translation="MNWNELRAFVSVARHASFSKAAEELHITQPAVTKRVQALEGSLS
                     ARLFDRVGKRVHLTDAGTRLLPRAEHLLREMSDTQRLLRNLKHEVSGVLSLATSHHVG
                     LHRLAPILREFTQRYPDVRLDIRFEDSEAAHDLVREAESELAVVTLDPLGDTELAYEH
                     LWHDPLSFVVAAEHALAGSSKLTLTELARHPVIMPGLGTYTGRIVAEVFSAAGVPLTP
                     AMSTNYLETIGMLVSTGLGWSVLPASMMSADLVSIRTEAPSLHRSLGLVTHPRRTLSN
                     AAQAFTAVALEHRDAISASAPAPDSGLGGA"
     gene            complement(3381..4451)
                     /gene="aroC"
                     /locus_tag="AC16QF_01265"
     CDS             complement(3381..4451)
                     /gene="aroC"
                     /locus_tag="AC16QF_01265"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007159018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR6447208.1"
                     /translation="MAGNTFGQLFTVTTFGESHGPALGAVVDGCPPGLALTEADLQAD
                     LDRRAPGRSRYTTQRREPDQVKILSGVFEGVTTGTPIGLVIENVDQRSADYGAIKEVF
                     RPAHADLTYQEKYGVRDYRGGGRASARETAMRVAAGAIAKKYLKERLGLQVRGRLSQI
                     GDVRVGFKSWDVVEDNPFFCADPEAVDAIAALIDGLRREGDSIGAEVTVVASLVPVGL
                     GEPVFGKLDAEIAGALMSINAVKAVGIGDGFDVVGQRGTAHRDEMTAEGFLSNHAGGV
                     LGGISSGQDIIARIALKPTSSLVTPGRSVDKAGNEVEVVTKGRHDPCVGIRATPIAEA
                     MVALVLMDHVMRHRAQNPERGH"
     gene            complement(4481..5332)
                     /gene="prmB"
                     /locus_tag="AC16QF_01270"
     CDS             complement(4481..5332)
                     /gene="prmB"
                     /locus_tag="AC16QF_01270"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: protein motif:HMM:TIGR03533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR6447209.1"
                     /translation="MTSLVGTIEEVADQFAASDIVYGHGTTNPWDEAVALVLGVTGLP
                     DDQAAADFEIDAESAAEVARLARRRIEDRVPIGYLVGSVNFYGQRFEVQPGIVIPRSP
                     IAQLIEGRLRPWLTRDPRSVLDMCTGSGCLGILCALAFPDAAVTLVDADPAAIALARR
                     NVAAHGLESRVEAVASDLFADLEPARFDLIVSNPPYVDEAEMAALPPEYRHEPAKGLA
                     GGADGLDLVDRLLESLPERLAPGGVFVCEVGASSPALLARYPEIPFLWPDLPDGGEGV
                     FILEGIV"
     gene            complement(5329..6219)
                     /locus_tag="AC16QF_01275"
     CDS             complement(5329..6219)
                     /locus_tag="AC16QF_01275"
                     /inference="COORDINATES: protein motif:HMM:NF027559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4892 domain-containing protein"
                     /protein_id="HIR6447210.1"
                     /translation="MRGILFTVLGFLSAAAWSEPDVPGSRDPLGLARFPHAWIVAYER
                     DTEMLPREFVVSRVDKTRRDVRAERKVRAEADLESVTYRIPPGTPAAEVAAHYRRLLG
                     ADVLFSCGGRDCGRSNHWANYIFEQPILYGPDQNQSYLAVQHAGHLLGLYVIERGNKR
                     VYAHLQVFRPGQPVAVAVNQVLTERLAGNGYVVVEGVTPLPNGTLPPSSRDVLAGLAP
                     DLGIFAGHRVYVVCHLYGSQSTEQLMEASSACSSTAAELLARPGGPALVGFGAGPLLP
                     RGPTSTRRVELVLPHRLHHQ"
     gene            complement(6263..8143)
                     /gene="htpG"
                     /locus_tag="AC16QF_01280"
     CDS             complement(6263..8143)
                     /gene="htpG"
                     /locus_tag="AC16QF_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HIR6447211.1"
                     /translation="MSEQPAQETFGFQTEAKQLLKLMIHSLYSNREIFLRELISNASD
                     AIDKLRFRSISDPSLAGDDAEFAITVSFDEDAGTVTVADNGIGMTRDEVIEHLGTIAK
                     SGTAEFFAQLSGDQKKDAALIGQFGVGFYSAFIVADEVEVLTRGAGTEQGTRWVSKGE
                     EDFSVADAPDVPRGTRVTLHLKEDAKEFADFFRLQSVVRRYSDHIGVPVRMPKVGDGE
                     DAGPEYETVNSAKALWTRSRSEVEDDEYQEFYKHVSHDFDDALGWSHNRVEGKLEYTS
                     LLYIPKHAPFDLWNREAPRGLKLYVQRVFIMDEAEQFLPLYLRFVKGVVDSNDLPLNV
                     SREILQQDDRVTTIRNALTKRVLNVLDAMAANEPEKYQSFWDEFGEVLKEGAGEDFAN
                     RESLCKLLRFASTAEGGATKSVSLDAYLERKVEGQDKIYFLVADSYEAARNSPHLEIF
                     KQRGIEVLLLFDRIDEWLMSALPRYGEVEFQDITRGGLELPGDASDPEAAEKPEATDD
                     LAQRIKAVLGDRVESVRSSKRLTESPACIVLDEHAMGPQMRRIIAASGQQMPEDKPHF
                     EYNASHPLVLKLLEEQDEDRFSDLVWILFDQASLAEGSVPKDSGAYVSRLNRLLLDLL
                     NG"
     gene            complement(8201..9076)
                     /gene="sucD"
                     /locus_tag="AC16QF_01285"
     CDS             complement(8201..9076)
                     /gene="sucD"
                     /locus_tag="AC16QF_01285"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR6447212.1"
                     /translation="MSILVDSSTRVICQGFTGAQGTLHSEQAIAYGTQVVGGVTPGKG
                     GSEHLGRPVFNTVADAVEATGATASVIYVPAPFCKDSILEALDAGIELVVCITEGIPT
                     LDMLAVKAKLDTTSSRVIGPNCPGVITPGECKIGIMPGEIHLPGRVGIVSRSGTLTYE
                     AVKQTTDRGFGQSSCVGIGGDPIPGSHFIDILALFEADPATEAIVMVGEIGGTAEEEA
                     AEFIRSNVSKPVVSYIAGVTAPPGKRMGHAGAIIAGGKGTAAEKFAALEAAGVRTVRS
                     LAEIGDAIAEVTGWS"
     gene            complement(9073..10242)
                     /gene="sucC"
                     /locus_tag="AC16QF_01290"
     CDS             complement(9073..10242)
                     /gene="sucC"
                     /locus_tag="AC16QF_01290"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIR6447213.1"
                     /translation="MNLHEYQAKGLFADYGLPVSQGYAVDTADEAVAAAERIGGGRWV
                     VKVQVHAGGRGKAGGVKLADSLDEVRAFADQWIGKNLVTFQTDEHGQPVSKIYVESCT
                     DIDRELYLGAVVDRGTRRVVFMASTEGGVEIEKVAEETPEKILRVAIDPFVGPQPYQG
                     RQLAFRLGLEGAQIREFTNLFMGLSRLFQELDCSLLEINPLVITTAGNIHCLDAKVNI
                     DGNAMFRQPALAAMRDVSQEDEREAEAAEYDLNYVALDGNIGCMVNGAGLAMGTMDLV
                     KIHGGNPANFLDVGGGATKEAVSEAFKIILSDENVKAVLINIFGGIVSCAIIAEGIVG
                     AVQEVGVEVPVVVRFEGNNAPRGREVLAASGLNIIAAESLADAARKAVAAAGGRP"
     gene            complement(10255..>11087)
                     /locus_tag="AC16QF_01295"
     CDS             complement(10255..>11087)
                     /locus_tag="AC16QF_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6447214.1"
                     /translation="GSEVVVLEALDEFLPMTDERIARDALKIFRNQGLDIRLGTRVMG
                     AEVTDGEVTVTYQDQDGDQSLKVDKLIVAVGRRPFTEGLLAPDCGVNLDERGFLYVND
                     LCATDAPHVYAVGDVVRGPMLAHKGMEEGVMVAERIAGFKPLVNYECVPAVIYTHPEV
                     AWVGKTEQELKASGDEYNVGSFPFTASGRAMAANDVQGQVKVLADAETDRILGVHVLG
                     PQASELIAQAVIAMEFDACAEDLGLTMFAHPTLSEAVHEAALSVAGHAIHTVNRKKQK
                     "
BASE COUNT         2024 a         3727 c         3350 g         1986 t
ORIGIN      
        1 gtccaggccg gcaacgagct cggcccgcag cgacgacgcc tcggcgagag ggccgaactg
       61 tatggccggg cgcaggttgg cgaaaagctc gagcccggcg cgcaggccga gcagccccct
      121 ttccggccgc tgcaaggtag gcagatcgtc ccacctgggc ccaccgacag cccccagcag
      181 cacggccctg gcctcgcggg cctggcgcag cgtctcgtca ggcagcggag aaccggtctc
      241 gtcgatagcg atgcccccga ccagcccttc ctcgaaagcg acgctcacgc cgaacttcaa
      301 agcggccgcg cccatcactg cctgagcaac cgggacgatc tctgcgccga tcccgtcgcc
      361 gggcagaaca agaattttat gattttcagt cacttgtgag atctttgcaa aatcaggttg
      421 gatgactctc gaagagccag ggcatggccc gacgatggcg ctcctcgaag gcccgaatcg
      481 cgtccacgtt ggccagggtc atgccgatgt cgtctagccc ctccaccaag gcgcgtttgc
      541 gattcgcgtc gatggcaaac tcgtatcgct cccccatccc ggcgatcacc tcctggcccg
      601 gcaggtccac ggtgagctca agcggctctt ctccagcaga gcgaacgaac aactcttcaa
      661 taatttcttc ttctaaaaca ataggaagta aaccattttt aaagcaatta cttagaaaaa
      721 tatcagcaaa gctgggggcg atcacgcagc ggaacccgta ctccagcagc gcccataccg
      781 cgtgctcacg actcgaaccg cagccgaagt tggcccgggc cagcaggatt gagccaccct
      841 gatatcgggg gtcgttgagc acgaaagcac ggttgatgcg gcgctcgttc ggcgtgatgc
      901 cgatgtcgcc ctcgtcttcg aaacgccagg cgtcgaagag gttatccccg aagcccgtcc
      961 ggtggatgga tttgaggaac tgcttgggaa tgatctggtc ggtgtccacg ttggcccggt
     1021 ccagcggaac cacgatgccc gttaccttat cgaacgcctt cacaggtact ccctcacgtc
     1081 aacgaatctg ccggcgatgc cggccgcggc ggccatggcc gggctcacca gatgggtgcg
     1141 gcctccgtgc ccctggcgcc cctcgaagtt gcgattcgaa gtggaagcgc agtgctcccc
     1201 gggcagcagc cgatccggat tcatggcgag acacatcgag caccctggcg cgcgccactc
     1261 gaagccggcg gccaggaaga cgtcggccaa accttccgct tcggcctggg ctttgacgag
     1321 accggaacca ggcaccacca gcgcctgctt gagactcccg gcaacccgcc tgcccttcac
     1381 cacccgcgcc gccgcgcgca ggtcctcgat gcgggagttg gtgcaggagc cgatgaaaac
     1441 ccggtcgacg gcgatctctg tcatgggctg atcactgcca agacccatgt attcgagcgc
     1501 gcggcgcaac ccgtcggatc gttgcgggtc gtccacgctg tctggattcg gggtgctggc
     1561 ggaaatgccg cacaccatct cgggcgaggt tccccaggtc acctggggct cgatctccga
     1621 ggcgttcagc tccagatccc gatcgaaatg cgcttgcgag tcggttacta gccctcgcca
     1681 gtatgcctcg gccgcatccc aggccgcccc aacgggcgcg aacggtcgct cgcgcaggta
     1741 ttccagcgtg cgatcgtcca cggccaccag gcccgccctg gcacccgcct cgatggacat
     1801 gttgcacagc gtcatgcgcc cttccatggt cagggagcgg attgcggacc cctggtactc
     1861 gatggtgtgt cccgtcgctc ccgcggtccc gatgcggccg atgatggcca gaacgaggtc
     1921 tttggcatat acgccaacgc caagctcccc ttcagctgcc accctgaaat taagggcctt
     1981 gggctggacg aggcactggg tcgccagaac gtgctcaaca tcggaagtcc cgatcccctg
     2041 agccagcgcg gcaaaggcgc catgggtgga ggtgtgggag tctccgcaaa cgatggtcat
     2101 tcccggcagg gtggcgccct gctccgggcc caccacgtgg acgataccct ggcgccggtc
     2161 ggtgatgccc tcgatgccca gctcacgcct gtcggtgggg acgttgtgat ccggggtcgc
     2221 gaggttggcg tccaatcgcc agggcttacg gcccgcgaga gccagacctt cgaaagcctg
     2281 aggcgaggtc acttcgtgca gcaggtgacg gtcgatgtag atcatggtct gaccgtcttc
     2341 gcgcacgccc accacatgag cgtcccatat cttgtcgtaa agggtttttg cggccacgtc
     2401 gctacggcta aaaagtagat ttgcacatcg tagaagccgg cttagaataa aacaaattca
     2461 taatttttat acttttcata ataatatgga atgatttgca gtgaactgga atgaattgag
     2521 ggccttcgtt tccgtggcgc ggcacgcgag cttctccaag gcagcggagg agctgcacat
     2581 cacccagccc gccgtcacga aacgggtgca ggccctggag ggcagcctct cagcgaggct
     2641 gttcgatcgc gttggcaagc gcgttcacct cacagacgcc ggcacccggc ttctgcctag
     2701 ggccgagcac ctgctgcggg agatgagcga tactcaacgg ctcctgcgca acctcaagca
     2761 cgaagtctcc ggcgtgctga gcctcgccac gtcgcaccac gtgggtctgc atcggctcgc
     2821 ccccattctg cgcgagttta cccagcgcta cccggacgtt cgcctggaca tccgcttcga
     2881 ggactcggaa gccgcccacg atctggtgcg ggaggcggaa tccgagctgg cggtggtcac
     2941 gctggatccc ttgggtgaca ccgagctggc ttacgagcat ttatggcacg acccgctgag
     3001 cttcgtggtc gccgccgagc acgcgctggc cggctcgagc aagctcacgc ttaccgagtt
     3061 ggcgcgccat ccggtgatca tgcccggcct gggaacgtac acggggcgaa tcgtcgcaga
     3121 ggtattctca gcggccgggg tgcccctgac gccggccatg tcgacaaact atctcgaaac
     3181 catcgggatg ctggtcagca ccggcctggg ctggagcgtg ctgcctgcaa gcatgatgag
     3241 cgcagatctg gtctccatcc gtaccgaggc gccgtcgctg catcggtcgc tgggcctggt
     3301 aacgcatccg cgacgcacgc tatccaacgc ggctcaggca tttaccgcgg tcgccctgga
     3361 gcaccgcgat gcgatttccg ctagtgcccc cgctccggat tctgggctcg gtggcgcatg
     3421 acgtggtcca tcagcaccag ggcaaccatg gcttccgcaa tgggcgtggc gcgtatcccg
     3481 acgcacgggt cgtgacgccc tttggttacc acctccacct cgttgccggc tttgtccact
     3541 gaccgtccgg gggtgaccag gctggacgtc ggcttgagcg cgatccgcgc gatgatgtcc
     3601 tgacccgagg agataccgcc cagcacgcct ccggcgtggt tgctcaggaa accctcggcc
     3661 gtcatctcgt cgcgatgcgc cgtgcctcgc tggccaacca catcgaagcc gtcgcctatc
     3721 cccaccgcct tgaccgcgtt gatgctcatc agcgcccccg cgatctcggc atcgagctta
     3781 ccgaaaaccg gctcgccgag gcccaccggc accaagctcg ccacgaccgt cacctccgca
     3841 ccgatggaat caccctccct gcgaaggcca tcgatgagcg cggcgatggc atcgacggct
     3901 tccggatcag cgcagaagaa aggattgtcc tccaccacgt cccagctttt gaagccgacc
     3961 cgaacgtcac cgatctgcga gagccgaccg cgcacctgca gcccgagccg ttccttgaga
     4021 tacttcttcg cgatggcgcc cgccgcgacg cgcatggccg tctcccgggc cgatgcccgg
     4081 ccgccacccc gatagtcgcg aacgccgtac ttttcctggt aggtcaaatc cgcgtgggcc
     4141 ggacggaaca cctccttgat cgcgccgtag tcggcgctgc gctggtcgac gttttcgatc
     4201 acgagcccga tgggcgtgcc cgtggtgacg ccttcgaaaa cgccggaaag gatcttcacc
     4261 tgatcgggct cccgacgctg ggtcgtgtac ctggaacggc cgggggcgcg gcggtcgaga
     4321 tccgcctgca ggtcggcttc cgtcagggcg aggccgggag ggcaaccgtc caccacagcc
     4381 cccagcgctg gcccgtggct ttcgccaaag gtcgtcaccg tgaaaagctg tccaaacgtg
     4441 ttcccggcca tcagtcaccg ctcctcgata gcgaggcgaa ttagacaata ccctcgagaa
     4501 taaacacacc ctccccgcca tccggcagat cgggccagag gaagggaatt tccgggtagc
     4561 gggccagcag cgccggcgag ctcgcaccca cctcgcacac gaagacgccg cccggagcga
     4621 ggcgctccgg caacgactcc agcagccgat cgaccaggtc gagcccatcc gcaccgccag
     4681 cgagaccctt cgccggctcg tgccggtatt ccgggggaag cgccgccatc tccgcctcgt
     4741 ctacgtaggg cggattgctc acgatcaggt cgaagcgggc cggctccaga tcagcgaaga
     4801 gatcggaggc cactgcctct acccgagact cgagcccgtg agcggcaacg ttgcgtcgcg
     4861 cgagcgcgat ggcagccgga tccgcatcca ccagggtcac cgccgcgtcg ggaaacgcaa
     4921 gggcgcacag gatgcccagg cacccggaac ccgtgcacat gtccaggacg ctgcgcggat
     4981 cacgggtcag ccacggacgc agtcgaccct cgatgagctg ggcaatgggc gagcgcggaa
     5041 tgacgatgcc gggctgcacc tcgaagcgtt gcccgtagaa attcacggag cccaccaggt
     5101 aaccgatcgg cacgcgatcc tcgatacgac gccgggcaag gcgcgccacc tccgccgccg
     5161 actccgcgtc gatctcgaaa tcggctgctg cctgatcatc cggaaggccg gtcactccca
     5221 gcacgagagc gacggcctca tcccagggat tcgtggtccc gtggccgtaa acgatatcgc
     5281 tggccgcaaa ctgatcagca acctcctcga tcgtgccgac cagactggtc attggtgatg
     5341 caggcggtgc ggaaggacga gctcgacccg ccgggtcgac gtgggcccgc gcggcagcag
     5401 aggacccgcc ccgaacccca cgagcgccgg ccccccgggt ctggcaagca gctcggcggc
     5461 ggtgctactg caggcgctgg acgcctccat gagctgctcg gtcgactgcg atccgtacag
     5521 gtggcagacc acgtaaacac gatgcccggc aaaaatcccc agatccgggg cgagcccggc
     5581 aagcacgtct ctggacgacg ggggaagcgt gccgttcggc agcggcgtca ccccttcgac
     5641 aacgacgtag ccgttgcctg ccagccgctc ggtgaggacc tgattgacgg ccaccgctac
     5701 cggctggcca ggccgaaaga cctgcagatg ggcatagacc cgcttgttgc cgcgctcgat
     5761 cacgtacagc ccgagcaggt gcccggcatg ctgcacggcg agataagact ggttctggtc
     5821 cgggccatac aggatcggct gctcgaaaat gtagttggcc cagtggttgc tccgcccaca
     5881 gtcacggccc ccacaggaga agagcacgtc cgctccgaga agccgccgat agtgggccgc
     5941 aacttccgcc gcgggcgtgc ccggggggat tcggtaggtg acgctctcaa gatccgcctc
     6001 ggctcggacc ttgcgttcgg cgcggacgtc tcgccgcgtc ttgtccacac ggctgacgac
     6061 gaattctctc gggagcatct cggtgtcgcg ctcgtaggcg acgatccagg cgtggggaaa
     6121 ccgggccagg cccagcggat cccgagagcc aggcacgtcc ggctccgacc atgccgctgc
     6181 ggaaagaaag cccagaactg tgaagagtat gccgcgcatg atcatcgacc cggacgtgca
     6241 gcgccttcgg gcgctgccta tcctagccgt tcagaagatc cagcaacaat cgattcagcc
     6301 ggctcacgta ggcgccggag tccttcggca ccgatccctc ggccagagac gcctgatcga
     6361 agagtatcca gacgagatcg gagaaacggt cctcgtcctg ctcttctagg agcttcagca
     6421 cgaggggatg agacgcgttg tactcgaagt gaggcttgtc ctccggcatc tgctgaccgg
     6481 aggcggcgat gatccgacgc atctgcgggc ccatggcgtg ctcgtctaga acgatgcagg
     6541 ccggcgactc cgtcagccgc ttggaagacc taacgctctc cacacgatcg cccagaacgg
     6601 ccttgatccg ctgagccagg tcgtccgtcg cctcgggctt ctccgcggct tcagggtcgc
     6661 tcgcgtcgcc gggaagctcg aggccgcccc gggtgatgtc ctggaattcc acctcaccat
     6721 atcgcggcaa tgcggacatc agccactcat cgatgcgatc gaagagcagc agcacctcga
     6781 tgccacgctg cttgaatatt tccaggtgag ggctgttacg ggcagcctcg taggagtcgg
     6841 ccaccaggaa gtagatcttg tcctggcctt ccaccttgcg ctcgaggtag gcatcgagcg
     6901 acaccgactt cgttgcgccg ccctcggccg tgctcgcaaa tctgagcagc ttgcagaggc
     6961 tttcccggtt ggcgaaatcc tcgccggcgc cctccttgag cacctcgcca aactcgtccc
     7021 agaacgattg gtatttctcg ggctcgttgg ccgccattgc gtccagcacg ttcagcacgc
     7081 gcttggtcag ggcattgcga atcgtcgtga cgcgatcgtc ctgctgaaga atttcccgcg
     7141 acacgttcag gggtaggtcg ttggagtcca ccacgccctt gacgaagcgc aggtagagcg
     7201 gaaggaactg ctcggcctcg tccatgatga acacccgctg cacgtagagc ttgagccccc
     7261 gcggcgcttc ccgattccaa agatcgaacg gggcgtgctt gggtatgtag agcaggctgg
     7321 tgtactcgag cttgccctcc acccggttgt gactccagcc aagggcatca tcgaagtcgt
     7381 gagaaacgtg cttgtagaac tcctggtact cgtcgtcctc gacctcgctg cgcgaccggg
     7441 tccacagcgc cttggccgag ttgacggttt cgtactccgg gccggcgtcc tctccatcac
     7501 cgaccttggg catccggacg ggaaccccga tatgatccga gtaccggcgg acgacgctct
     7561 gcagccggaa gaaatcggcg aactccttgg catcttcctt gaggtgaagg gtcacgcggg
     7621 tccctctcgg cacgtcaggc gcatccgcga cggagaagtc ctcctcgcct ttggagaccc
     7681 agcgggttcc ctgctcggtg ccggcgcctc gggtcagcac ctcgacctcg tcggcaacga
     7741 tgaaagcgct gtagaaacca acgccgaact gcccgatcag ggccgcatcc ttcttctgat
     7801 ctccgctgag ctgagcaaag aactccgccg taccggactt ggcaatggtg ccgaggtgct
     7861 cgatcacctc gtcgcgcgtc atcccgatgc cgttgtcggc aaccgtcacg gtgcccgcgt
     7921 cctcatcaaa agacacggta atggcaaact cggcgtcgtc acctgccagg ctggggtccg
     7981 aaatggaccg gaagcgaagc ttgtcgatgg cgtccgaggc gttggatatg agctcgcgca
     8041 gaaagatttc cctgttcgag tacagagagt gaatcatcag cttgagcagt tgcttggctt
     8101 ccgtctggaa gccgaaggtc tcctgggctg gttgctcaga catggctacc cgtcacgtga
     8161 tccccgcgtt ggcatctata tggctgctaa agccggaatt tcaagaccag ccggtcacct
     8221 cggcaatggc gtcgccaatt tctgccaggc ttcgcaccgt gcgcacgccc gcagcctcaa
     8281 gagccgcaaa tttctcggcc gccgtgccct tgcctccggc gatgatcgcc ccggcatgac
     8341 ccatacgctt gcctggcggg gcggtaaccc cggcgatgta ggaaaccacg ggtttggaga
     8401 cattcgatcg aatgaactcg gccgcttcct cctccgccgt tccaccgatc tcccccacca
     8461 tcacgatggc ttcggtcgcc gggtctgctt cgaacagcgc caggatgtcg atgaagtggc
     8521 tcccaggaat gggatcaccg ccgatgccga cgcagctgct ctggccgaat ccgcggtccg
     8581 tagtctgctt taccgcttcg taagtgaggg ttcccgagcg ggacacgatg cctacgcggc
     8641 ccggcagatg gatctcgccc ggcatgatgc cgatcttgca ctcgcccggc gtgatgactc
     8701 cagggcagtt gggcccgatg acgcggctgc tcgtcgtgtc gagcttggct ttcaccgcga
     8761 gcatgtccag ggtcggtatg ccttcggtga tgcagaccac gagctcgatg ccggcgtcca
     8821 gcgcctcgag gatggagtcc ttgcagaacg gagcgggcac gtagatcacc gacgcggtgg
     8881 cgccggtggc ttccacggca tccgccaccg tattgaacac cggtcgtcca aggtgctcgg
     8941 aaccaccttt gccgggcgtg acgccgccga ctacctgagt gccgtaggca atggcctgct
     9001 cgctgtgcag ggtgccctga gcgcccgtga agccctgaca gatgaccctt gtgctgctgt
     9061 cgaccagaat gctcatggcc gtcctcctgc cgctgccacg gctttccggg cggcgtcggc
     9121 cagactctcc gcagcgatga tgttcaaacc gctggcggcg agcacctcgc gtccccgcgg
     9181 ggcgttgttg ccctcgaaac gcaccaccac gggcacctcg acccccactt cctggacggc
     9241 tcccacgatc ccttcggcga tgatggcgca cgagacgatg ccgccgaata tgttgatcag
     9301 gacggccttc acattctcgt ccgagagaat gatcttgaag gcctccgaaa ccgcctcctt
     9361 ggtcgcgccg ccgccaacgt cgaggaagtt ggccgggttg ccgccatgaa tcttcaccag
     9421 atccatggtg cccatggcca ggcccgcgcc gttcaccatg caaccgatgt tgccgtcgag
     9481 cgcgacgtag ttcagatcgt actcggcagc ttcggcttcc cgttcatcct cctgggagac
     9541 gtcgcgcatg gccgcaaggg ctggctggcg aaacatcgcg ttgccgtcga tgttgacctt
     9601 cgcatcaagg caatgaatat tccccgccgt ggtaatcacc agcgggttga tctccagaag
     9661 cgagcaatcc agttcctgaa atagcctgga taagcccatg aaaagatttg taaattccct
     9721 tatctgagcg ccctctagac caagccggaa ggccagctga cggccctgat agggttgtgg
     9781 ccctacaaag ggatcgatgg caacgcgaag gatcttctcc ggcgtctcct ctgccacctt
     9841 ctcgatctcg acgccaccct cggtggacgc catgaaaacc acccgccggg tgccccggtc
     9901 gacgacggcg cccaggtaga gttcccggtc gatgtccgtg cagctttcca cgtagatctt
     9961 gctgacgggt tggccgtgct catcggtctg gaaagtcacc aggttcttgc cgatccactg
    10021 atcggcgaag gcgcggacct cgtcgaggct gtctgccagc ttgacgccac ccgccttgcc
    10081 gcgaccgcct gcgtgcacct gcaccttgac cacccaacgc cccccgccga tccgctcggc
    10141 ggcggccacg gcttcgtccg ccgtgtctac cgcatacccc tgcgacaccg gcagcccata
    10201 gtcggcaaac agccccttgg cctgatactc atgtagattc ataagacttc cgttctattt
    10261 ctgtttctta cgattgaccg tatgtatggc atgtccggcg acgctcagcg cggcttcgtg
    10321 gacggcttcg ctcaatgtcg gatgagcgaa catcgtaagc cccagatcct ccgcgcacgc
    10381 gtcgaactcc atggcgatga ctgcctgggc aatgagctcc gaagcctgag gccccagcac
    10441 gtgcacgccc agaatccggt cagtctcggc gtcagcaagc accttgacct ggccctgcac
    10501 gtcgttggct gccattgccc ggccactggc cgtgaacggg aaactgccca cgttgtactc
    10561 atccccgctg gctttgagct cctgctcggt cttgcccacc caggcaacct cggggtgtgt
    10621 gtagatcacc gccggtacgc actcgtagtt gacgaggggt ttgaagccgg cgatgcgctc
    10681 ggcgaccatg acgccctcct ccatgccctt gtgcgcgagc atcgggccac gaaccacgtc
    10741 gcccacggcg tagacgtggg gggcatcggt agcgcagaga tcgttgacgt aaaggaagcc
    10801 gcgctcgtcc agattgaccc cgcaatcggg cgcgaggagc ccttcggtga agggccgcct
    10861 gcccaccgcc acgatgagct tgtccacctt gagcgactga tccccatcct gatcctgata
    10921 cgtcacggtt acctcaccat cggtgacctc cgcccccatt acccgggtcc ccaggcggat
    10981 atcgagcccc tgattgcgaa agatcttcag cgcatcccgg gcaattcgct cgtcggtcat
    11041 gggaagaaac tcatccagcg cttccagaac caccacctcg gagccga
//
LOCUS       DBNHLM010000028         3368 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_960562, whole genome shotgun sequence.
ACCESSION   DBNHLM010000028 DBNHLM010000000
VERSION     DBNHLM010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_960562"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2273
                     /locus_tag="AC16QF_01300"
     CDS             <1..2273
                     /locus_tag="AC16QF_01300"
                     /inference="COORDINATES: protein motif:HMM:NF037144.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR6447215.1"
                     /translation="GQPANVSELPPGMLKERLESLPPTARQSALRWLQDFSFPAADAA
                     HMSADDAGGIYYVDEAPDGVEEAEGAAAEPPTSGALSAAETFALHSKPGASKVLFLDF
                     DGHVIEGTAWNANSSRPDTYYARPFDTDGSPSSFGSAELETIHEVWHRVAEDFAAFDV
                     DVTTEDPGSFGPSVGHVLVTKDDDELGNAMPSAGAGGVAYVGVFGRSNYTYYQPALVY
                     YDNLGNGHPPYVAEASSHEFGHNLSLSHDGTSTTSYFQGLGSTSSFVSWAPIMGVGYY
                     RQVTQWSNGEYADANNTQDDLSIITGHLGPRGDDHGDDILSASLLVTDGAGTISHSNP
                     QSDPGNFNPENKGIIDQQGDVDVFGLQVGTGTVDITVTPAWDAFTRSMRRGANLDIQA
                     RLLDDTGTVIATSDPGDDTDASIQVQLDAGYYYLAVTGVGNSSVPYSDYASQGMYFIS
                     GQAVTGEPNGAPTANVDTAGTAEDFAVDVAVLANDSDPDGDALSILSVSSPANGTTDI
                     SGTMVIYTPNANFAGNDAFTYSVGDGRGGSDSATVTVTVTGMNDAPSAVNDAAATASD
                     TPVSVAVLSNDTDVDGDALSVVGVGAAGNGTVSHDGSAVQYTPAAGFSGTDTFGYTVS
                     DGQATASATVTVTVSAPAPATPGSFAATDGANGTAALTWTDSANETGYEVERESLHRK
                     RGVWVGTSLVATPGQDATSHTDASGTGNYRYRIRAVNGAGASSWTPWQEVAVTETSGS
                     GGGGGGTKGGGNGRKK"
     gene            complement(2280..>3368)
                     /locus_tag="AC16QF_01305"
     CDS             complement(2280..>3368)
                     /locus_tag="AC16QF_01305"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6447216.1"
                     /translation="AAVLLIPLVFVYLCGPNLGAPQWNSLIGDLLPEARRGRFFALRT
                     RLSSVASLAALVIAGLVLEAFDKSGDAYWGFVVIFLLAGAARCVSAYHLNAMTEPTRN
                     VAGLLAPDDLSVWRRVRHSSLLRFSLFFAAMQFAVAISGPYFTLYMLRDLALSYVEFM
                     VLSFVSVTIQFLTLNRWGRLADLFGNRLILITTGSIITIIPSLWLFSTHYGWLLVVQA
                     VSGLAWSGFTLSATAFVFDLTPSEHRATLFAVHNVLAAVAVFLGAGAGALIAAHMPSS
                     LSGFGMEVPLLTPLYGVFFVSCLMRLTVAALFLRTINEVRRVRPMSPGGLIFRVTRMH
                     PISGLIYEIVGRSRPRSAAPTSRDPAER"
BASE COUNT          677 a         1171 c         1088 g          432 t
ORIGIN      
        1 aaggacaacc ggccaacgtc tccgagctgc cccccggcat gctcaaggag cgtctcgaga
       61 gcttgccccc cacagcccgc cagagcgcgt tgagatggct ccaggacttc agcttccccg
      121 cggccgacgc cgctcacatg agcgccgatg acgccggcgg catctactac gttgacgaag
      181 cgcccgacgg cgttgaggaa gccgaaggcg cggccgccga gccgccgacc agcggggctt
      241 tgagcgcggc tgagactttc gccctgcaca gcaagcccgg cgcctccaag gtgctctttc
      301 tcgacttcga cggccacgtc atcgaaggca cggcctggaa cgccaacagc agccggcccg
      361 acacctacta tgcccgcccg ttcgacaccg acggttcgcc gtcgagcttc ggctcggcgg
      421 agctggaaac cattcacgag gtctggcacc gggtggcgga ggacttcgcc gccttcgacg
      481 tggacgtaac caccgaggat ccgggcagct tcggcccgtc cgtcgggcac gtgctcgtca
      541 ccaaggacga cgacgagctc ggcaacgcca tgcccagcgc gggcgccggc ggggtcgcct
      601 acgtgggcgt gttcggccgc tcgaactaca cctactacca gcccgcgctc gtctactacg
      661 acaacctggg caacgggcat cccccgtacg tggcggaagc ctcctctcac gagttcggcc
      721 acaacttgag cctctcccac gacgggacga gcaccaccag ctacttccag ggactcggaa
      781 gcacctcgag cttcgtctcg tgggcaccca tcatgggggt gggctactac cgccaggtga
      841 cccagtggag caacggcgag tacgcggacg ccaacaacac ccaggatgac ctgtccatca
      901 tcaccggcca tctcggcccc cgtggtgatg accacggcga cgacatcctg tcggccagcc
      961 ttctcgtcac cgatggcgcg ggcaccattt ctcacagcaa cccgcaatcg gatccgggca
     1021 acttcaatcc ggaaaacaaa ggcatcatcg accagcaggg cgacgtcgac gtcttcgggc
     1081 tccaggtcgg taccggcacg gtcgacatca cggtgacgcc cgcatgggac gccttcaccc
     1141 gcagcatgcg ccggggcgcg aacctggaca tccaggcccg gctgctggac gacaccggca
     1201 ccgtcatcgc cacgtccgat cccggcgacg acaccgatgc gagcattcag gttcagctgg
     1261 acgccggcta ctactacctc gccgttaccg gcgtcgggaa cagctcggtc ccctactccg
     1321 actacgcgag ccagggcatg tacttcatat cggggcaggc ggtcaccggc gagcccaacg
     1381 gcgcgcccac cgccaacgtc gacacggccg gaacggccga ggacttcgcc gtggacgtcg
     1441 cggtgctggc caacgacagc gatccggacg gcgatgcgct ctcgatcctg tccgtcagct
     1501 cgccagcaaa cggcaccacc gacatcagcg gcaccatggt catctacacg cccaacgcaa
     1561 acttcgccgg caacgacgcc tttacctact cggtcggcga cggccggggc ggctcggact
     1621 ccgcaacggt gaccgtgacc gtgaccggga tgaacgacgc gccgagcgcg gtgaacgacg
     1681 ctgccgccac ggcgtccgac accccggtat ccgtcgcggt gctgagcaac gacacggacg
     1741 tcgacggcga cgccttgagc gtggtcgggg tcggcgcggc cggcaacggc acggtcagcc
     1801 acgacggctc cgccgtgcag tacacgcccg cagccgggtt ctccggaacg gatacgttcg
     1861 gctacaccgt cagcgacggt caggcaaccg catccgccac ggtgacggtc accgtctccg
     1921 ccccggcacc ggcaacgccg ggaagcttcg ccgccacgga cggcgcgaac ggcacggcag
     1981 cgctgacctg gaccgacagc gcgaacgaga ccggctacga ggtggaacgc gaatcgctgc
     2041 acaggaagcg cggcgtttgg gtcggcacct ccctggtggc aacgccgggg caggacgcca
     2101 ccagccacac ggacgcctcg ggaaccggaa actaccgcta ccggatacgg gcggttaacg
     2161 gcgccggcgc ttcctcctgg accccatggc aggaggtcgc cgttacggag accagcggca
     2221 gtggtggcgg aggtggcggc accaagggcg gcggcaacgg ccgcaagaag tagccaccgt
     2281 cagcgctcgg cggggtccct ggacgtgggc gccgccgagc gcggccggga tcggccgaca
     2341 atctcgtaga tcaaccccga gatcggatgc atgcgggtga cccggaagat gagccccccg
     2401 ggggacatcg gcctcacccg ccgaacctcg ttgatggttc gcagaaacag cgcggcaacc
     2461 gtgaggcgca tcaggcacga tacgaagaag acgccgtaaa gcggcgttag cagaggcacc
     2521 tccataccga aaccgctcag ggaagaaggc atgtgcgcgg cgatcagggc gccggctcct
     2581 gccccgagga acaccgccac cgcagcaagg acgttgtgca ccgcgaacag ggtcgcgcgg
     2641 tgttcggatg gggtcaggtc gaacacgaaa gcggtggcgc tcagcgtgaa ccccgaccag
     2701 gcgagcccgc tgaccgcctg aacaaccaga agccacccgt agtgggtaga aaacaaccac
     2761 agcgacggga tgatggtgat gatgctgccg gtggtaatca ggatcagccg gttgccgaaa
     2821 aggtccgcca gccgccccca acggttcagg gtgaggaact ggatcgtgac cgacacgaag
     2881 ctcagcacca tgaactcgac gtagctcagc gccagatccc tgagcatgta gagcgtgaag
     2941 tacggccctg atatcgccac cgcgaactgc atcgcggcga agaacagcga gaaccgcagc
     3001 agactagagt gccggacccg ccgccacacc gaaaggtcgt ccggcgccag aagccccgcc
     3061 acgttgcgcg tcggctctgt catggcattg agatggtagg ccgagacgca gcgcgcagcg
     3121 cccgcgagca ggaagatcac cacgaacccc cagtaggcgt ctccggactt gtcgaacgcc
     3181 tcgaggacga gaccggcgat caccagcgcg gccagggagg caacgctgga caaccgggtg
     3241 cgcagggcaa agaaacggcc gcggcgggcc tccggaagca gatcaccgat gaggctgttc
     3301 cattggggtg cgccgaggtt tggtccacac aggtagacga acaccagagg aatcagcagc
     3361 accgcggc
//
LOCUS       DBNHLM010000029        32175 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1379874, whole genome shotgun sequence.
ACCESSION   DBNHLM010000029 DBNHLM010000000
VERSION     DBNHLM010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 32175)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 32175)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32175
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1379874"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /gene="atpB"
                     /locus_tag="AC16QF_01310"
     CDS             <1..781
                     /gene="atpB"
                     /locus_tag="AC16QF_01310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR6447217.1"
                     /translation="HPDGTWGFAQSAQEAAAMGFNAINVDSMGWSIGLGLVFVAVFYT
                     AARKATSGVPGGLQNFVEIIIEFIDEITHSIFTHKNDMIAPMALTVFVWVFLMNLMDL
                     IPVDWIPGLAAAMGISHMKIVPTTDVNITMAMAGSVFGLIIYYSIKCKGVGGFLGELA
                     FHPLPKAAAPANLFLEGVTLIAKPLSLGLRLFGNMYAGEMIFILIALLFPAGIVYGLL
                     GGVLQWGWAVFHVLIITLQAFIFAVLTVVYLAQAHDVMEEH"
     gene            839..1075
                     /gene="atpE"
                     /locus_tag="AC16QF_01315"
     CDS             839..1075
                     /gene="atpE"
                     /locus_tag="AC16QF_01315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HIR6447218.1"
                     /translation="MELEVLLYVAGGLMMGLGAIGAAIGVGVLGGKFLEGVARQPELL
                     PMLRTQLFIVLALVDAVPMIAVGIGLYTLFAVAG"
     gene            1145..1615
                     /locus_tag="AC16QF_01320"
     CDS             1145..1615
                     /locus_tag="AC16QF_01320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019694394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR6447219.1"
                     /translation="MNINWTIVGQSLTFFVFVWFCWKFIWPPIVKAMRERQEAIAEGL
                     ASSERAAKDLELAQERATDQLREAKDEAHKIIEQARGRANQMIDEAKNEAREEVERIR
                     EGARAEIDQEINRAKEALRGQVAALAVSGAERVLGSAIDAERHRELLDQLATDL"
     gene            1627..2166
                     /locus_tag="AC16QF_01325"
     CDS             1627..2166
                     /locus_tag="AC16QF_01325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIR6447220.1"
                     /translation="MAELATLARPYANAVFATARDEGNLERWSRMLGFLAAAVREPEV
                     RRLIGSPDVSDEQKAFRLADVCGDELNDKARKLVQVLAGNKRLGLIDEILEQFEHKRA
                     QDEQTLDVEVVTAYPLSDDQAARLKQALGEKYAKEVNLSSVVNEDLIGGAIIRAGDTV
                     IDGSVRGRLIKLAESLQRI"
     gene            2231..3775
                     /gene="atpA"
                     /locus_tag="AC16QF_01330"
     CDS             2231..3775
                     /gene="atpA"
                     /locus_tag="AC16QF_01330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR6447221.1"
                     /translation="MQQLNPSEISDIIRQRIEGLDVRAQAQNEGTIVGVSDGIVRIHG
                     LADAQYGEMIEFDGGLYGMALNLERDSVGVVILGDYEGLSEGMTARCTGRILEVPVGR
                     ELLGRVVDSLGNPIDGKGPLNASETAPIEKVAPGVIARQSVDQPVQIGLKCIDSMVPI
                     GRGQRELIIGDRQIGKTAIAIDAIINQKDSGIRCVYVAIGQKMSTIANIVRTLEEHGA
                     MENTIVVAASASDPAPMQFIAPYSGCSMGEYFRDNGEDALIIYDDLTKQAWAYRQISL
                     LLRRPPGREAYPGDVFYLHSRLLERAARVNADYVEQYTGGKVKGRTGSLTALPIIETQ
                     AGDVSAFVPTNVISITDGQIFLETDNFNSGLRPAMSPGISVSRVGGSAQVPFIKKIAG
                     GIKLALAQYRELAAFSQFASDLDDATRRQLEHGRRVTELMKQKQYAPMSVAEMGVSLY
                     AANEGYLVDIAVDQVLEFEQGLLAYMNSEKADFMRRINETGDYDDGIVAEMRQGIEAY
                     KAQANF"
     gene            3786..4646
                     /gene="atpG"
                     /locus_tag="AC16QF_01335"
     CDS             3786..4646
                     /gene="atpG"
                     /locus_tag="AC16QF_01335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIR6447222.1"
                     /translation="MAVGKEIKKKIGSVENTKKITSAMQMVAASKMRRTQERMREGKP
                     YSEKIRAVIGHLANSNPEYRHVYMEPREISRIGYLVVSTDKGLCGGLNINLFRTLIRD
                     MAEWHAKGKEIDLGLIGNKAAAFFRSVGGNIVAAMNNVGEDPALADLIGGIKVMLDAY
                     EEGKIDRLFIASNEFVNTMTQRPVVRQLLPLDPETDDSMRHRWDYIYEPDARQLIEGL
                     VTRFMESQVYQAVVENGACEQAAKMVAMKSATENAEKLIDELTLIYNNARQAAITQEI
                     AEIVGGAAAV"
     gene            4678..6060
                     /gene="atpD"
                     /locus_tag="AC16QF_01340"
     CDS             4678..6060
                     /gene="atpD"
                     /locus_tag="AC16QF_01340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR6447223.1"
                     /translation="MSSGRIVQIIGAVIDVEFSREDVPKVYDALKVSSTGLTLEVQQQ
                     LGDGVVRAIAMGPSDGLSRGLEVTNTQQPISIPVGEETLGRIMNVLGEPIDEKGPVNA
                     QQKMPIHRKPPSYEDQKGGNEILETGVKVIDLICPFAKGGKVGLFGGAGVGKTVTMLE
                     LIRNIATEHSGLSVFAGVGERTREGNDFYHEMEESGVLDKISLVFGQMNEPPGNRLRV
                     ALTGLTLAEQFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPNLAEEMGV
                     LQERITTTKTGSITSVQAVYVPADDLTDPSPATTFAHLDSTVTLSRAITDLGIYPAVD
                     PLDSTSRQLDPLVVGEEHYQVARGVQQVLQRYQELRDIIAILGMDELSEEDKQLVYRA
                     RKMQQFFSQPMFVAEPFTGQPGVYVSREDTVRSFKGILEGEYDHLPEQAFYMVGTIEA
                     AIEKAETLSA"
     gene            6072..6494
                     /locus_tag="AC16QF_01345"
     CDS             6072..6494
                     /locus_tag="AC16QF_01345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014873131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HIR6447224.1"
                     /translation="MAMTFHCDIVSAEREIFSGRVTMLSAIGTIGELGILPGHAPLLT
                     GIRPGPVRLRMDNGEEEVFFASGGYLEVQPGVVTVLADTALRAEDIDEAAAAAAQQDA
                     ERALREQTADIEFSAAAAMLAEATARQRTLAELRKRRR"
     gene            6665..8056
                     /gene="glmU"
                     /locus_tag="AC16QF_01350"
     CDS             6665..8056
                     /gene="glmU"
                     /locus_tag="AC16QF_01350"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR6447225.1"
                     /translation="MSSSPRLEVIVLAAGQGKRMNSRLPKVLHPLAGRPLLAHVLETV
                     DELDPAGIHVVIGHGADLVREAIGAAALRWVVQTEQLGTGHAVQQALPDIDDSAVVLV
                     LCGDVPMVTAQTLRRCVTGAGNGAMAVITAELSDPAQLGRILRGTDGAVRGIVEYKDA
                     SAAEKAISEINSGIIAAPAGILRDLLAEVRPDNAQGEYYLTDIVALAVSRGIEVLGLK
                     AERPEEVLGVNDRNQLAVLERRYQLERAGELMAAGATLADPSRLDVRGAVTVGADCFI
                     DVNVVFEGTVVMGREVSIGPGSVIRDSQLGDGVRVEAHTLVDGARVEAGCSLGPFARI
                     RPGTQLGEGVKIGNFVETKKAVLGAGSKASHLTYLGDAILGANCNVGAGTVTCNYDGV
                     DKHRTTIGDGVFVGTNSTLVAPVTLGDDAFVAAGSTITGDVEKGELAVGRGRQRNVQG
                     WVRPGRRDRADED"
     gene            8072..9904
                     /gene="glmS"
                     /locus_tag="AC16QF_01355"
     CDS             8072..9904
                     /gene="glmS"
                     /locus_tag="AC16QF_01355"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020190289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR6447226.1"
                     /translation="MCGIVGAVADRNVAPILLEGLQRLEYRGYDSAGLAVIDGAGEMD
                     CRRRVGKVKELAGDLSARRCDGHMGIAHTRWATHGVPSENNAHPHVSNGRIALVHNGI
                     IENFEELREELQSEGYAFASETDSEVIVHLVDSYFDKGLSLLEAVRSAVKRLEGAYAI
                     GVIALADPDRIVAARMGSPLVVGKGLGENFIASDPLALRPVTDRFVFLEEGDLVEITR
                     DEISIWNVENESVIRASVRVELGHDDVDRGNYRHFMQKEIYQQPEVLAATLEGRIGRT
                     RVLEPAFGVQAGQIFDKVQAVTIVACGTSYYAGSIARYWIEELARIPCQVEIASEFRY
                     RQVAVTPGNLFVTISQSGETADTLAALRVAKELGYVSTLTICNVATSTLVRESDIALM
                     TQAGTEVGVASTKAFTTQLADLLLLTILIARRHGMDAAREQELVEALAGLPNAVEQVL
                     GLDGRIRVLAERFMDRSNALFLGRGPMFPVAMEGALKLKEISYIHAEGYPAGELKHGP
                     LALVDENMPVVAVAPNDELLEKVQSNLQEVRARGGALYVFADERTGFKEEPGVTVIPL
                     PPVHDTLAPIVYVVPLQLLAYHVAVLKGTDVDQPRNLAKSVTVE"
     gene            9979..12162
                     /gene="uvrD"
                     /locus_tag="AC16QF_01360"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             9979..12162
                     /gene="uvrD"
                     /locus_tag="AC16QF_01360"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016971704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HIR6447227.1"
                     /translation="MDVTNILEHLNKDQREAVSAPLGNALVVAGAGSGKTRVLVHRIA
                     WLIEAERVSPHGILAVTFTNKAAAEMRARIETLLDLSVRTMWVGTFHGIAHRLLRMHW
                     QEAGLKQNFQILDADDQLRLVKRVMRALDIDEQKWPARQAVWFINAQKDEGRRAKDVA
                     AGDDLFQITHKKIYESYESLCESGGLVDFAELLLRSHELWLNRSDLLAHYQERFQNIL
                     VDEFQDTNTIQYAWLRVLAGSSANIMAVGDDDQSIYGWRGARIENIHQFSQDFAGASL
                     IRLEQNYRSTATILKAANALIRQNVDRLGKELWTEGGDGEPIRVYSAYNDLDEARFVA
                     ERTAEALASGASADDVAVLYRSNAQSRVLEEAFLRAQIPYRIYGGVRFFERAEIKNAL
                     AYMRLTVDRHSDTAFERVVNTPARGIGDKTVEAIRAQARARNVSLWQACKEALAENVL
                     ASRTAAKIADFLGLIDGLAEQTAELDLHEVADACIQSSGLLEYHMRERGERGLARKEN
                     LEELVSACRQFSGEQVFPLLADAAEQTSILEEFLDQVALDAGDRQAEAGPAVQMMTLH
                     SAKGLEFPMVFLAGMEEGLFPHRMSAEEPGRMEEERRLCYVGITRAKQTLYLTHAETR
                     RLHGTDTYNRPSRFLLEIPTELVHEVRMGGAVQRPYGRPAEAGHAVGAEGGLRMGQRV
                     LHAKFGEGVVLQAEGSGERARVQVNFAGAGAKWLMLGYANLQALD"
     gene            12180..13265
                     /locus_tag="AC16QF_01365"
     CDS             12180..13265
                     /locus_tag="AC16QF_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR6447228.1"
                     /translation="MMVMRILALVLMFGLAGCGAPQQTAPEAVPDPDKVYRWKLITTW
                     PKNLPALGMAPERLAENLRVMSRGRLDIRVYGAGELVGAFEVFDTVRQGTAEMGHGAA
                     YYWRGKLPVAAMFATVPFGMMAQEMNGWLRYGGGMELWREAYEPFGLVPLAAGNSGVQ
                     MAGWFNKEINSLEDLQGLKMRIPGLGGEVLKRAGGVPVSMAGSEIYTAMQTGVIDATE
                     WVGPYNDLAMGLQDVARYYYYPGWHEPGPTLEAIVNKAAWDSLPEDLQRMVEVAAAAA
                     NEDLLSEFTARNASALKTLLSKHNVELRRLPDDVLAKLKVESEAVVVEATGDSDLARR
                     IRESYGRFLSDVREYHAISEQAYINAR"
     gene            13299..14138
                     /locus_tag="AC16QF_01370"
     CDS             13299..14138
                     /locus_tag="AC16QF_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6447229.1"
                     /translation="MELKATEYSVASGIATVRLNRPHRLNAWTGRMHSEYRWCIDRAE
                     RDPAVRAIVVTGNGRGFCVGGDAEALEGHARKGGYDPGTPARLAAPGYGRAPEFDASF
                     AYHYGLSKPVIAAVNGPAAGVGLALACFADLRFAAAGVKLTTAHGKLNLPAEYGLSWL
                     LPRMIGLTRANDLLLSSRVFLAEEALAMGLVNAVYPQAELMDEVYAYTRELIRTVSPA
                     SLAQTRWQIYRDLHRSVAESVRASEQLLDEMMRQEDYREGVAAFLEKRAPSWSGSAGE
                     TGE"
     gene            complement(14179..15363)
                     /locus_tag="AC16QF_01375"
     CDS             complement(14179..15363)
                     /locus_tag="AC16QF_01375"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6447230.1"
                     /translation="MSERPTTITVSRVWDGEALCDADAVRIENGRIAAVGDARELSKG
                     AVAVHCPGATALPGLIDAHVHLELNPDNHKPPESTSPDEVPRMLERAAAMVRAGITTA
                     RDLGGGAWLELGIRDKTAAGQAIGPRLLCSGQPITCPGGHCHFWGGEAGSAEEALSVL
                     ERQVTQGVDLIKVMATGGRMTNGSDPLAAQFDLETLSEITAAARAHGLPVAAHCHGTT
                     GIELAARAGVSTIEHCSWAGAEGWASDFQEPVANVILERGVWVSPTVNRGWQRMLDSG
                     EGRVLGRVRAAYRAMLELGIPFIASTDAGIPGVFHHHLPLALKVFALIAELSPQAALR
                     TATSSAARALGVHGMTGRLVPGLAADILVVDGNPLDDLDALANPVGVWARGRRVVDPH
                     GA"
     gene            complement(15360..16763)
                     /locus_tag="AC16QF_01380"
     CDS             complement(15360..16763)
                     /locus_tag="AC16QF_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6447231.1"
                     /translation="MVELIPIFMFLAVFAALLAGYPVALTLAGVALLFALGGMAGGTF
                     DGRDLGFLPGRLFGIITNPTLIAVPLFVFMGVMMEKTRIAENLLSSLSRLMAGTPGGL
                     AFAVIVVGMLMAASTGIVGASVVTLGLMSLPTMLRDRYDPAFASGTISATGTLGQIIP
                     PSIALVLLGEVLSNAYQQAQLNQGIFAPDTVSVGDLFAGAVVPGLILVGLYLAYTMLV
                     AFLRPGTAPAASSVPDAVPDAVPDAVPDGSGWQLLGGLLPPMLLIVAVLGSILAGYAT
                     PTEAAGVGAAGATILAAAYRALDVGVLRQVCRSTFDTTAMVFFILVGASLFSLVFRGY
                     GGDDLVHSWFNDMPGGTWGALAIVMLVIFLLGFMLDFIEITFVVVPIVGPVLLAMGVD
                     PIWLGVLIAVNLQTSFLTPPFGFALFYLRGVAPPTVSTADIYRGVAPFVLLQILLLCL
                     LFIWPSRATWLPERIYR"
     gene            complement(16768..17229)
                     /locus_tag="AC16QF_01385"
     CDS             complement(16768..17229)
                     /locus_tag="AC16QF_01385"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR6447232.1"
                     /translation="MAWLTALMVVLMVTVVVLRYAFDQGAIPIQEAIVYLHATVIMLG
                     IPCALRDGVHVRVDILHSRLSERARGIIDVAGHLLFLVPTCLLILYFSIPYARNAWLV
                     LEGSPEVGGIPAVFLLKTLIPVMAVALLLQGLAETVRRLPIILPSHFGSER"
     gene            complement(17283..18191)
                     /gene="ubiA"
                     /locus_tag="AC16QF_01390"
     CDS             complement(17283..18191)
                     /gene="ubiA"
                     /locus_tag="AC16QF_01390"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007160213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HIR6447233.1"
                     /translation="MEKTRQPPLSAVIAYARLMRLDRPVGTLLLLWPTLAALWVAAEG
                     LPPVDLIVVFAVGTFLMRAAGCVVNDYADRNLDPGVARTANRPLATGAVSTNAALGAF
                     FVLAFLALTLLLFLNTLTRWFAVAGLAIAVAYPFMKRWTYLPQVVLGAAFSWGIVMAF
                     TAVRHVVPEPGWLLFLASVLWIVAYDTMYAMVDREDDLRVGIKSTAILFGEADRLMIG
                     LLQTGTILTLVLVGQRLGYHSAYFWGIAVAAGLFCYQQYLIRDRSREGCFRAFANNVW
                     VGFSLFVGVAWETMTRGAFQPGGGLS"
     gene            18351..18518
                     /locus_tag="AC16QF_01395"
     CDS             18351..18518
                     /locus_tag="AC16QF_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0043448 - alkane catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HIR6447234.1"
                     /translation="MRKWQCIVCGWIYDEAEGYEEEGIAPGTAWEDVPEDFVCPECGV
                     SKEDFEMIEVG"
     gene            18535..19803
                     /locus_tag="AC16QF_01400"
     CDS             18535..19803
                     /locus_tag="AC16QF_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012593.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR6447235.1"
                     /translation="MNLTIRILLGMGLGLVLGSSIQAAEFDPSHWVHVYLVGGLIDAG
                     GQIFVRSLQLMVVPLVFVSLVCGAASLSATDSIGRVAGKTVALYLFTTAVAISIALLL
                     ATIVQPGAGVAPASLGTDFQPAEPTSIKDTLVSIIPTNPVSAMAEGRMLQIILFALLL
                     GLALSRSGQAGRRLAGVFDDLNEALMKLITLLIKLAPIGVFCLMAKLFATLGWSEITR
                     LLQYFLTVVGALLIHVTLVYPTLLMVLGRVSPIRFFGKMREPLLVAFSTASSGATLPV
                     TLRTVEERVGVKNEVASFVVPLGATINMDGTAIMQGVATVFIAQLYGIELGMSQFLII
                     ILTATMASVGTAGVPGVGMIMLVMVLEQVGLPLDGIVFILAVDRLLDMLRTAVNVAGD
                     GMVATVVARSENELDLDTFEDPDAGRVIGS"
     gene            complement(19825..21837)
                     /gene="recG"
                     /locus_tag="AC16QF_01405"
     CDS             complement(19825..21837)
                     /gene="recG"
                     /locus_tag="AC16QF_01405"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019694287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR6447236.1"
                     /translation="MIAEQRVESLKGVGPSLKAKLERLGIFRVGDLLLHLPIRYQDRS
                     RVVPLRDVHPQQECLVQGQVVDSSLGYGRRRSWLVTIEDGTGYLGLRFFHFSKRQQSG
                     LQPGTYVRCFGEIRFGPRGGGPGLEMAHPEYRAFTSEPPDPDPELTPVYPTTKGLGQN
                     RLRGLIDQALSLEWPSDSGAPYQTLAYLHRPPADAPAADIERAQERVALDELTAYYLV
                     MKQRQRDRAKSRALPLPQSQQLGRELLRRLGFRLTHAQRRVITEVLEDLSSETPMLRL
                     LQGDVGSGKTVVAAFAAIRAAEHGRQTALMAPTEILAEQHYVNFSAWLEPLGIPVVLL
                     TGSQSAKERAARQQALEEGEALVAVGTHALFQDKVNFNALALTIVDEQHRFGVHQRMA
                     LRSKGAAPHQLVMTATPIPRTLTMALYADMDVSVLDELPPGRTPIDTRVISDARKEEV
                     IRSAADAMARGEQVYWVCPLIEDSEQLDAAAAEPTAELLAERLEPPIGLLHGRMTGEQ
                     KAKAMMDFKEGRTRLLVATTVIEVGVDVPNATLMVIENPERMGLAQLHQLRGRVGRGE
                     KASRCLLVYKTPLSAAARQRLSVMRESQDGFHIAEKDLELRGPGELLGARQTGEQQFR
                     VADLARHSHLIAEVLARGERLLQTDPTTARELLNAWAPEDNGHIAV"
     gene            complement(21867..22259)
                     /locus_tag="AC16QF_01410"
     CDS             complement(21867..22259)
                     /locus_tag="AC16QF_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR6447237.1"
                     /translation="MTKQTISTENAPAAIGPYSQAVRTGSLVFLSGQIPLVPATMDMV
                     AGDVIEQAHQVFRNLRAVASAAGGSLDDLVKVTVYLTDLNDFQSVNEVMTAYFSEPYP
                     ARAAIGVAALPRGAAVEVEGVMAHPGDG"
     gene            complement(22302..24584)
                     /locus_tag="AC16QF_01415"
     CDS             complement(22302..24584)
                     /locus_tag="AC16QF_01415"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR6447238.1"
                     /translation="MAKPARSDHQGSKHPGSRVVRKKTRAGEKPKLPQPESYFVGLLA
                     DESRRDDIAAPVSIESLTTLLSTYLEPDQVRRVERAHAYAINAHEGQFRRTGHPYISH
                     PLAVANILATMRMDHETIMAALLHDVIEDTDADRNALAQRFGKSVAEIVDGVSKLSKI
                     FRSRAEAQAENFQKMALAMAKDIRVIMVKLADRLHNMRTIGVMSREARQRIARETLDF
                     YAPIANRLGIHTMKVEFEELGFRALYPLRADRIGRAVRAARGNRKELVEELRKSLQDA
                     LDQESVTAEVIGREKHLYSIYRKMKAQHKPFAEIMDVFGFRVIVDEVEDCYRALGVVH
                     NTYKPVAGRFKDYIAIPKVNGYQSLHTTLFGMHGVPIEVQIRTRDMAAVADNGIAGHW
                     LYKSEGEDFQSSQQRARRWVKDLLDLQQRAGNPMEFVESLKIDLFPDEVYVFTPQGDI
                     LELPRGACAVDFAYAVHTDIGNRCVACRIDRNLAPLSQQLQSGQSVEIITSEGARPNP
                     DWLTFVVSSKARSGIRQALKAQQATDSIAFGRRLLNRSLAHANKSINDLDFRRLRRVF
                     TEFGVRRLDEVLARIGSGELMAYLVAQRLLAADDPDFQGVPVETGGPVAIGGGEGLVI
                     TYGRCCGPVPGDPIVGHMTPGKGFVVHMASCPNMQEVRRRRGNRELIPAYWANSPDNE
                     FLTTLRIQVNRKKGIIAELAAAVADADAGVENISVEERSAEVTSVIVNVSVTDRDTLA
                     RVMRKMRAIPSVLSIGRVSQ"
     gene            complement(24562..24834)
                     /gene="rpoZ"
                     /locus_tag="AC16QF_01420"
     CDS             complement(24562..24834)
                     /gene="rpoZ"
                     /locus_tag="AC16QF_01420"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003195508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR6447239.1"
                     /translation="MARITVEDCLENVENRFELVMLATRRARQMRRFGAEPLVPEEND
                     KPTVIALREIAEGLISNEMLDAQEASTEDEELEVSFGIGDGETGEI"
     gene            complement(24934..25566)
                     /gene="gmk"
                     /locus_tag="AC16QF_01425"
     CDS             complement(24934..25566)
                     /gene="gmk"
                     /locus_tag="AC16QF_01425"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR6447240.1"
                     /translation="MSQASRQGLLFIVSAASGTGKTSLVRALIAQDGNLAVSISHTTR
                     PRRPGDQEGVDYHFVEPKLFREMAQRGDFLEDADVFGNRYGTSKRTVDEQLRTGVDVI
                     LEIDWQGARQVRAAYPDATSIFILPPSRAALVQRLEGRGLDAANVIEARSDKAREEIS
                     HYGEYDYLVVNDDFATALSELTQIIAASRLGLQPQRERLGALLKDLLRQE"
     gene            complement(25563..26417)
                     /locus_tag="AC16QF_01430"
     CDS             complement(25563..26417)
                     /locus_tag="AC16QF_01430"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR6447241.1"
                     /translation="MTAFARVQGSAKGLSLIWEMRSVNHRFLETQFRLPEQMRPLEHG
                     LRETLRRHVKRGKFDCSLKIDRSTGGDGIELNRPLLLQLLAAVEQIRRDAPEIGPGNA
                     MDVLRWPGIIGDASTLEDEPLVQAATDLFEEALVQLIDNRQREGAQLASVISERLDEI
                     DSIAREVKALTATMSHEVRSRLTQRLNDLKVQADEGRLDQEVALLAQRADVAEELDRL
                     VIHVEEARSNLRGPGPHGRRLDFLTQELNREANTLGAKSVLAQTSQKAVDLKVAIEQI
                     REQVQNVE"
     gene            26572..27291
                     /gene="rph"
                     /locus_tag="AC16QF_01435"
     CDS             26572..27291
                     /gene="rph"
                     /locus_tag="AC16QF_01435"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HIR6447242.1"
                     /translation="MTETQVQRPSGRAPSEMREVRFTRGFTKHAAGSVLVEFGDTKVI
                     CTASVENSVPGFLRGKGVGWVTAEYGMLPGATHVRTDREAARGKQGGRTQEIQRLVGR
                     ALRASVDMQALGERTIRLDCDVIQADGGTRTASITGSALALADAIASAGCNGALKELV
                     ASVSVGLWRGQPVLDLDYAEDSTAETDMNVVMLESGGFIEIQGTAEAGAFSDSELMSM
                     LALARSGTAELLRRQRQALSS"
     gene            27288..27956
                     /gene="pyrE"
                     /locus_tag="AC16QF_01440"
     CDS             27288..27956
                     /gene="pyrE"
                     /locus_tag="AC16QF_01440"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR6447243.1"
                     /translation="MSASRNDRSLVAFLIEQGVLAFGDFTLKSGRRSPYFFNLGALNT
                     GSALARLGAAYAERIRRLDVPFDVVFGPAYKGIPIAVSTAEALAALGGDVGWAFNRKE
                     AKSHGEGGLFVGADVSGRVMLVDDVLTAGTAVREAAALIGSAGAEIAGVVIALDRQER
                     VAPNSGETAVSALELELGAPVVSILKLQDVIEYLDLDVDRDKHRAELIREMKRYRDEH
                     CVLN"
     gene            28052..28657
                     /locus_tag="AC16QF_01445"
     CDS             28052..28657
                     /locus_tag="AC16QF_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447244.1"
                     /translation="MVAILLMPSWASANRLYRYLDASGHIAISHTIPSDRVKFGYEIV
                     DAGSGRVIEVVEAQKSPEEIARLEQEAAARKACEDALERVYSVYQSELDIENAEEQML
                     ESLEQRIANAEFNLERAQSQKDGFEATAARLERSGKELDTSLIANIERSQVQVRSLET
                     EIVQRRHEQAKAKERFARDRALFRQEICVEQTAISAVTETN"
     gene            complement(28682..29266)
                     /gene="slmA"
                     /locus_tag="AC16QF_01450"
     CDS             complement(28682..29266)
                     /gene="slmA"
                     /locus_tag="AC16QF_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HIR6447245.1"
                     /translation="MPPRSGRRDEILSAFARMLESNPGTRITTAALAGQIGVSEAALY
                     RHFPSKAKMIEGLIEFIEESLFSRINRILAEEASAASRCRAVLWLLLSFAERNPGLAR
                     LLVGDALQGETQRLRARMRQVFERLETELRSIIRDWSAARVPAPTLGATTAANLLLAS
                     AEGRISQFVRSEFRALPTSGWEEQWKALEGAVFG"
     gene            complement(29256..30176)
                     /gene="argB"
                     /locus_tag="AC16QF_01455"
     CDS             complement(29256..30176)
                     /gene="argB"
                     /locus_tag="AC16QF_01455"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR6447246.1"
                     /translation="MPISESQARNIASVLTEALPYIQRFHGGTVVVKYGGNAMVDETL
                     KTTFARDVVLLKLVGLNPIIVHGGGPQIGELLDRLNIPTRFVKGMRVTDAETMDVVEM
                     VLGGLVNQDIVSLINKNGGRAVGITGKDGDLIRAHRLALSADAAELAPSEIIDIGHVG
                     EVESVNPAVLDALVRSNFIPVIAPIGVGPDGESYNINADLVAGRLAEELRAEKLILLT
                     NTAGILDANGQTLTSLSVAEINELIAAGTIGSGMLPKAQCAVSAAERGVGSTQIIDGT
                     IPHALLLEIFTDAGIGTQITTAEASGSDAS"
     gene            complement(30181..>32175)
                     /locus_tag="AC16QF_01460"
     CDS             complement(30181..>32175)
                     /locus_tag="AC16QF_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HIR6447247.1"
                     /translation="MVGPEGASINQRGYVYMAAQIGEPGSRAGVLFAVVSPDFFLSGL
                     AWLDERIGRVRIEQLSESVDPIEIFGWGGQSDPAAAEIVTLENQKWNLVFEPGSAILQ
                     PVTKMVDLALPFGVALLLVIAGGYLSTTRVTKRLRQAAAAMPVQAQPPEEAPAEETAV
                     EEEAEPVGEEAEPGAGAASTLPSRGFTDDAKDNFGIEVSEEVSLGEMGIDLDPQIFRA
                     YDIRGIVTDNLTEDVVYWIGRAFAAEALAQEQTRAAVGRDGRHSSEALRTALIKGLTD
                     GGLDVADVGEVPTPLLYFATHALDLGTGIMITGSHNPPEYNGLKMMIAGDTLAEERIQ
                     RLKERLENNELSEGEGDVEEVDIVRYYIDSALDDIVVAQQLKVVVDCGNGVAGAVAPV
                     LLEEAGCEVVPLYCDVNGDFPNHHPDPADPKNLEDLITVVQAESADLGLAFDGDGDRL
                     GVVTSSGQIIWPDRLLMLFAQDIVSRNPGADIVYDVKCSRHLNAIIGDHGGRPIMWKT
                     GHSHIKAKLKETGALLGGEFSGHICFNDRWYGFDDALYSAARLLEILGAEGGTVDEVF
                     EQFPTGQSTPELKIQTTEDAKFGIIEELSRSADFGDGTVTSIDGVRVDYPDGWGLIRA
                     SNTSPVLTLRFEADDAEALARIQSDFAVQIAKVDPSLKIS"
BASE COUNT         5696 a        10101 c        10757 g         5621 t
ORIGIN      
        1 ccatcctgac ggcacctggg ggtttgccca aagcgcccag gaggcggcgg ccatggggtt
       61 caatgccatc aacgtggatt ccatgggatg gtccatcggg ctcggcctgg ttttcgtggc
      121 cgttttctac accgcggcgc gcaaggctac gtccggcgtc ccgggcggtc tgcagaactt
      181 cgtcgagatc atcatcgagt tcatcgacga gataacccac agcatcttca cccacaagaa
      241 cgacatgatc gcgcccatgg cgctcaccgt cttcgtctgg gtgtttctca tgaacctgat
      301 ggacctgatc ccggtggatt ggatcccggg gctggccgca gccatgggga tcagccacat
      361 gaagatcgtt ccgaccacgg acgtgaacat caccatggcc atggccgggt cggtgttcgg
      421 cctcatcatc tactacagca tcaaatgcaa aggtgtcggg ggtttcctcg gcgagctggc
      481 cttccacccc ttgcccaagg cggccgcgcc ggcgaacctg tttctagagg gggtgaccct
      541 gattgccaaa ccgctttctc tgggcctgcg actgttcggc aacatgtatg cgggggagat
      601 gatcttcatc ctcatcgcgc tgctgtttcc ggcgggcatc gtctacgggc tgctgggagg
      661 cgtgctgcag tggggatggg cggtcttcca cgtcctcatc atcaccctgc aggctttcat
      721 ctttgcggtc ctgacggtgg tttacctggc acaggcccac gacgtgatgg aagagcacta
      781 agctctcctg atgggaaatt ccgagagcgg aaacaacaac tcgtaactgg gagtgatcat
      841 ggaactagaa gttctcttat atgtagccgg tggactcatg atgggtctgg gcgccattgg
      901 ggccgccatc ggcgtgggtg ttctcggcgg caagtttctg gaaggcgtcg cgcgccagcc
      961 cgaactgctt ccgatgctcc ggacccagct cttcatcgtg ctggcgctgg ttgacgccgt
     1021 gcccatgatc gcggtgggta tcggtctgta caccctcttt gcggtggcag gttagccacc
     1081 cgacggaact gccggccacg gtgtgccgtg acggtctgtc gaaacgaatc gaagaggtga
     1141 gttcgtgaac atcaactgga ccatcgtcgg gcagagcctg acgtttttcg ttttcgtctg
     1201 gttctgctgg aaattcatct ggccgcccat cgtcaaagcc atgcgcgagc gtcaggaagc
     1261 cattgccgag ggactggcat cgtccgagcg tgcggccaag gacctggagc ttgcccagga
     1321 gcgcgccacg gatcagctgc gcgaggccaa ggacgaagcc cacaagatca tcgagcaggc
     1381 ccggggccgg gccaaccaga tgatcgacga ggccaagaac gaggcgcgcg aagaggtcga
     1441 acgcatcagg gaaggcgccc gggcggagat cgatcaggag atcaaccgcg cgaaagaagc
     1501 gctccggggt caggttgccg cgctggcggt ttccggcgcc gagcgggtgc tgggttccgc
     1561 catcgatgcc gagcggcacc gcgagctgct cgaccagctt gccaccgacc tgtgacggtc
     1621 tgaccaatgg cagaactagc aaccctcgcc cgcccttatg ccaacgcggt atttgccacg
     1681 gctcgcgacg aaggcaacct ggagcgctgg tcccggatgc tcggctttct cgccgcggct
     1741 gttcgggagc ctgaggttcg tcggctgatc ggctcaccgg acgtgagcga cgagcagaag
     1801 gcgttccggc ttgccgatgt ttgcggcgac gagctgaacg acaaggcgcg caagctcgtc
     1861 caggtgctgg ccgggaacaa gcggctcggg ctcatcgacg agatactcga gcagttcgag
     1921 cacaagcgcg cccaggacga gcagaccctc gatgttgagg tggtgaccgc ctaccccttg
     1981 agcgacgatc aggccgccag gctgaagcag gcgctggggg aaaagtacgc caaggaagtg
     2041 aacctctcca gcgtcgtgaa cgaagatctg attggcggcg ccatcatcag ggccggggat
     2101 accgtcatcg acggctccgt gcgcgggcgt ttgatcaagc tggcagagtc gctgcagcgt
     2161 atctgaatac agaggccttc ggcgggaacg acaccgtcga ggctccaaga aagagactga
     2221 ggaattcgaa atgcaacaac tgaatccttc cgagatcagc gacatcatca ggcagcggat
     2281 tgaaggattg gacgtacgcg cgcaggcgca gaacgaaggc accattgtgg gcgtgtcaga
     2341 tggcatcgtc cgcatccacg gccttgccga cgcccagtat ggcgagatga tcgagttcga
     2401 cgggggcctc tacggaatgg cgctcaacct cgagcgcgac tctgtcggcg tggtgatcct
     2461 cggcgactac gagggtctct ccgaggggat gacggctcga tgcaccgggc ggattctcga
     2521 agtcccggtg ggccgcgagc tgctcggccg ggtggtggat tcccttggca accccattga
     2581 cggcaaaggg ccgctgaacg cctcggaaac cgcgcccatc gaaaaggttg cccccggcgt
     2641 gattgcgcgg cagtccgtgg accagcccgt tcagatcggg ctgaagtgta tcgactccat
     2701 ggtgcccatc ggccggggtc agcgcgagct catcatcggc gacaggcaga ttggcaagac
     2761 ggccatcgcc atcgacgcca tcatcaacca gaaggacagc ggcattcgtt gtgtctacgt
     2821 ggccatcggg cagaagatgt cgacgattgc caacatcgtc cggaccctcg aagagcacgg
     2881 cgccatggag aacaccatcg tcgtggctgc tagcgcatcc gatccggcgc ccatgcagtt
     2941 catcgcgcct tactccggct gcagcatggg cgaatacttc cgggataacg gtgaagacgc
     3001 cctgatcatt tacgacgacc tcacgaaaca ggcctgggcc taccggcaga tctcgctcct
     3061 gctgcgccgg ccgccgggcc gcgaggctta tcccggcgac gtcttctatc tgcactcgcg
     3121 tttgctcgag cgtgcggcaa gggtgaacgc cgattacgtg gagcagtaca cggggggtaa
     3181 ggtcaagggc cggaccgggt ccctgacggc gctgccgatc atcgaaactc aggcggggga
     3241 cgtatccgcg ttcgtgccga cgaacgtcat ctccatcacc gatgggcaga tcttcttaga
     3301 gacggacaac ttcaactcgg ggctgcgccc tgccatgagc ccgggcatct cggtatcccg
     3361 ggtgggggga tccgcccagg tgcccttcat aaagaagatt gccggtggca ttaagctcgc
     3421 ccttgcccag taccgggaac tcgcagcgtt ctctcagttt gcttcagacc ttgacgacgc
     3481 gacgcggcgc cagctcgagc acggccggcg agtcacggag ctcatgaagc agaagcagta
     3541 cgcgccgatg agcgtagcgg aaatgggcgt gagcctgtat gcggcgaacg agggctacct
     3601 ggtggacatc gcggtcgatc aggtgctcga gttcgagcag ggcctgcttg cctacatgaa
     3661 ctccgagaaa gccgacttca tgcggcgcat caacgagacc ggcgactacg acgacggcat
     3721 cgttgcggag atgcgccagg gcatcgaagc ctacaaagca caagcgaact tctaaggcag
     3781 cggatatggc ggtcggcaaa gagatcaaga aaaagatcgg cagcgtcgag aacaccaaga
     3841 agattacgag cgcgatgcag atggttgcgg cgagcaagat gcgccgcacc caggagcgga
     3901 tgcgggaagg caagccctac tcggaaaaga tccgcgcggt catcggccat ctggcgaact
     3961 ccaacccgga gtaccggcac gtctacatgg agcctcgcga gatcagccgg atcggctacc
     4021 tcgtggtctc cacggacaag ggactctgcg gcggcctcaa catcaacctg ttccgcaccc
     4081 tgatccggga catggcggag tggcatgcca agggcaagga gatcgacctt ggcctgatcg
     4141 gcaacaaggc ggcggcgttc ttccgctcgg ttggtggaaa catcgtcgcg gccatgaaca
     4201 acgtcggcga ggatcctgcg ctggccgacc tcatcggcgg gatcaaggtc atgctcgacg
     4261 cttacgagga aggcaagatc gaccggctgt tcatcgccag caacgagttc gtcaatacga
     4321 tgacccagcg cccggtggtc cggcagctcc tgcccctgga cccggaaacc gacgacagca
     4381 tgaggcatcg ctgggactac atctacgagc cggacgcgcg ccagctcatc gagggcctgg
     4441 ttacccgctt catggaatcc caggtctacc aggcggtggt ggagaacggt gcctgcgagc
     4501 aggcggctaa gatggtcgcc atgaagagcg ccacggaaaa cgcggagaag ctcatcgacg
     4561 agctgaccct gatttacaac aacgctcggc aggcggcgat cactcaggag attgcggaga
     4621 tcgtcggcgg ggccgcggcc gtttagatag acagattaga ttgaagaggc acaaagcatg
     4681 agcagcggac gaatcgtaca gattatcggc gcggtgattg acgtcgaatt ctcgcgggag
     4741 gatgtcccaa aggtctacga cgcccttaag gtttcctcaa cggggctcac cctcgaggtg
     4801 cagcagcagc tcggtgacgg ggtggttcgt gccattgcca tggggccctc ggacggtctc
     4861 tcgcggggtc tggaggtgac caatacccag cagcccatct ccatccccgt gggtgaagaa
     4921 acccttggcc ggatcatgaa cgtgctcggc gagcccatcg acgagaaggg gccagttaac
     4981 gcgcagcaga agatgcccat tcaccgcaag ccgccctcat acgaggacca gaaaggcggc
     5041 aacgagattc tcgagacggg cgtcaaggtc atcgacctca tctgcccgtt cgccaagggc
     5101 ggcaaggtgg gcctgttcgg cggtgccggc gtcggcaaga cggtcaccat gctggagctg
     5161 atccgcaaca tcgccaccga gcactccggc ctgtcggtct tcgccggtgt gggcgagcgg
     5221 acccgggaag gaaacgactt ctaccacgag atggaggagt cgggcgtgct cgacaagatc
     5281 tcgctcgttt tcgggcagat gaacgagccg ccgggtaacc gtctgcgggt agcgctcacc
     5341 ggcctgaccc tggccgagca gttccgcgac gaaggtcgcg acgtcctcct ctttgtggac
     5401 aacatctacc gctacacgct ggcgggaacc gaggtgtccg cgctgctcgg ccggatgccg
     5461 tccgcggtag gctaccagcc aaacctggcc gaggagatgg gcgtgctgca ggagcggatc
     5521 accaccacca agaccggatc gatcacgtcc gtgcaggccg tttacgtgcc cgccgatgac
     5581 ctcaccgacc cgtctccggc caccaccttt gcgcacctgg attccacggt gacgctctcc
     5641 agggccatca cggacctcgg catctacccc gccgtggatc cgctggattc cacgagccgt
     5701 cagctcgacc cgctggtggt cggtgaggag cactatcagg tggcccgggg cgttcagcag
     5761 gtgcttcagc gctaccagga gctccgcgac atcatcgcca tccttgggat ggacgagctt
     5821 tccgaggagg ataagcagct ggtctatcgc gcgcgcaaga tgcagcagtt cttctcgcag
     5881 cccatgttcg tcgccgagcc ctttaccggc cagccgggcg tgtacgtctc ccgggaggat
     5941 acggtgcgca gcttcaaggg cattctcgag ggagaatacg accaccttcc ggagcaggcg
     6001 ttttacatgg tgggcaccat cgaagcggcg atcgagaaag ccgagacgct tagcgcctaa
     6061 ggagagcggc catggccatg accttccatt gcgatatcgt cagcgcggag cgcgagatct
     6121 tctccggccg ggtgaccatg ctgagcgcca tcgggaccat cggtgagctg ggcatcctgc
     6181 ccggccacgc cccgctgctg acgggcatcc gtccggggcc ggtccggctg cgcatggaca
     6241 atggcgagga agaggtcttc ttcgcctccg gagggtatct cgaggtccag ccaggggtgg
     6301 tcaccgttct tgccgacacg gcgctgcgcg cggaagacat cgacgaggcc gccgctgctg
     6361 ccgctcagca ggatgcggag cgggcattgc gggagcagac ggcggatatc gaattttccg
     6421 ctgccgctgc catgcttgcc gaggcgaccg cgcggcagag gacgctggca gagcttcgca
     6481 agcgccggcg ctgacgcgcc gggcgaatct gcccaaggtt cttgtccaat gcaggggtga
     6541 tctgtgtcac cctttattcg ttccccggtc ctataatcgt cagagcatag aggttcggcc
     6601 gcccgtgacg cgggtgcggt tcggcccggc ctgaaaccag ggactgtttc tgctcaacat
     6661 ggttatgtct tccagccctc gccttgaagt catcgtgctg gccgccggcc agggtaagcg
     6721 catgaactcg cggttaccga aagtgctgca cccgctggcc gggcgtcccc tgctcgccca
     6781 cgtcctggag accgttgacg agctagatcc cgccggcatt cacgtggtca tcggtcatgg
     6841 cgccgatctg gtgcgtgagg ccattggcgc ggcggcgctg cggtgggtgg tgcagaccga
     6901 gcagctgggc accggccacg ccgtgcagca ggccctgccg gacatcgacg attcagccgt
     6961 ggttctggtt ctgtgcggcg acgtgccgat ggtgacggcc cagacgctca ggcgctgcgt
     7021 caccggcgcc ggcaacggcg caatggccgt catcaccgcg gagctgagcg atccggcgca
     7081 gctggggagg atcctgcgag gtaccgacgg cgccgtgcgc ggcatcgtgg agtacaaaga
     7141 tgccagcgct gccgagaagg ccatctcgga gatcaattcg ggaatcatcg ccgcgcctgc
     7201 gggcattttg cgggacctgc ttgccgaggt ccgcccggac aacgctcagg gagagtacta
     7261 cctcaccgac atcgtggccc tcgcggtcag ccgcggtatc gaggtgctgg gtctgaaggc
     7321 ggagcggccc gaagaggtgc tgggcgtgaa tgaccgaaac cagctggccg tcctcgagcg
     7381 gcgctaccag ctcgagcggg ccggcgagct gatggccgcc ggggccaccc tggcggatcc
     7441 gtcgcgactg gacgtgcgcg gcgcggtcac cgtgggcgcc gactgcttca tcgacgtcaa
     7501 cgtcgttttc gagggcaccg tggtcatggg ccgggaagtg agcatcggtc ccggctcggt
     7561 cattcgggat tctcagctgg gcgacggtgt gcgcgtcgaa gcccacaccc tggtggacgg
     7621 tgccagggtc gaagccggtt gcagcctggg gccctttgcc cggatccgac cgggcacgca
     7681 gctgggcgaa ggcgtgaaga tcggcaactt cgtcgagacc aagaaggccg tgctgggtgc
     7741 aggaagcaag gccagtcacc tgacctatct tggtgatgcc atcctcgggg ccaactgcaa
     7801 cgtgggcgcc ggcacggtga cctgcaacta cgacggtgtc gacaagcacc gaacgaccat
     7861 tggcgacggc gtcttcgtag gcaccaacag cacgctggtc gccccggtca cgctgggcga
     7921 cgatgctttc gtggcggcgg ggtccacgat caccggcgat gtcgagaagg gcgagctggc
     7981 cgtggggcgg ggccgacagc gtaacgttca gggctgggta cgacctggcc ggcgcgaccg
     8041 cgcggacgag gattagcatg aggagcctgg catgtgcggg atcgtagggg ccgtcgccga
     8101 ccgcaacgtg gcgcccatcc tcttggaagg acttcagcgg ctggagtatc gcgggtacga
     8161 ctccgctggc cttgccgtga tcgatggggc gggtgagatg gattgccggc gtcgcgtcgg
     8221 caaggtcaag gagctcgccg gagacctgag cgcccggcga tgcgatggtc acatgggaat
     8281 tgcccacacg cgttgggcaa cccacggcgt tcccagcgag aacaacgccc acccccacgt
     8341 ctccaacggc cgcatcgcgc tggtgcacaa cggcatcatc gaaaacttcg aggagctgcg
     8401 cgaagagctt caaagcgagg gttatgcgtt tgcgtccgag accgactcgg aggtgatcgt
     8461 tcacctggtg gattcatatt tcgacaaggg attgagcctg ctcgaggccg tcaggtccgc
     8521 ggtcaagcgg ctggaaggcg cttacgccat cggcgtcatc gcgctcgccg accctgatcg
     8581 gatcgtcgcc gcgcgcatgg gcagcccgct ggtggttggc aaggggctgg gagaaaactt
     8641 catcgcttcc gacccccttg ccttgagacc ggtgaccgac cgattcgtgt tcctggagga
     8701 aggggatctc gtcgagatta cccgagatga gatctccatc tggaacgtgg agaacgagtc
     8761 ggtgatccgc gccagcgtcc gggtagagct cggtcacgac gacgtggacc ggggcaatta
     8821 ccggcacttc atgcagaagg agatctacca gcagccggaa gtgctcgcag cgactctgga
     8881 agggcgcata ggccgtaccc gggtgctcga gcccgcattc ggggtgcagg ccgggcagat
     8941 cttcgacaaa gtccaggcgg tgaccatcgt ggcctgcggg acgagctact acgcgggctc
     9001 catcgcgcgt tactggatcg aggagctcgc gcgtatcccc tgccaggtgg agatcgccag
     9061 cgaattccgc taccggcagg tggcggttac cccgggcaac ctgttcgtga ccatctccca
     9121 gtcgggtgag accgcggata ccctggcagc gctgcgcgtg gccaaagagc tcggctacgt
     9181 ctcgacgctc accatctgca acgtcgccac cagcacgctg gtgcgcgagt ccgacatcgc
     9241 gctgatgacc caggccggga cagaggtggg cgtggcgtca accaaggcgt tcaccacgca
     9301 gctggccgac ctgttgctgc tgaccattct cattgcccgc cggcacggga tggacgcggc
     9361 cagagagcag gagctggtgg aggcgctcgc cgggcttccc aatgccgttg agcaggtgct
     9421 cggcctcgac gggcgcatcc gcgttctggc ggaacgcttc atggaccgga gcaacgcgct
     9481 gtttctcgga cgcgggccca tgttcccggt ggcaatggaa ggcgcgctga agctgaagga
     9541 aatctcctac atccatgccg agggctatcc cgccggggag ctgaagcacg gccctctggc
     9601 gcttgtcgac gagaacatgc cggtcgttgc cgtggcgccg aacgacgagc tgctcgagaa
     9661 ggtgcagtcc aacctgcagg aagtgcgcgc gcgcggcggt gccctctatg tctttgccga
     9721 tgagcgcacc ggcttcaaag aagagcccgg ggtcacggtg atcccgctgc cccccgttca
     9781 cgacacgctg gctcccatcg tctacgtcgt gccattacaa ttgctcgcct atcacgtcgc
     9841 ggtgctcaaa ggcacggacg tggaccagcc aaggaatctg gctaagtcgg tcacggtgga
     9901 gtagtccgtt ccggccctaa gccgcgccgg gcggttccct gaacccccgt cccttggaca
     9961 ggcttagggc gggtagcagt ggacgtcacg aacattctcg agcatctgaa caaggaccag
    10021 cgggaggcgg tctcggcgcc tctgggcaat gccctggtgg tggctggcgc cggcagcggc
    10081 aagacgcggg tgctggttca tcggattgcc tggctgatcg aggccgagcg ggtatccccc
    10141 cacggcatcc tggccgtcac cttcaccaac aaagcggcgg cggagatgcg ggctaggatc
    10201 gaaacgctcc tggatctctc cgttcgcacc atgtgggtcg gcacgtttca cggcatcgcc
    10261 catcggctgc ttcgcatgca ctggcaggaa gcgggcctca aacagaactt tcagattctc
    10321 gacgccgacg atcagctgcg cctggtcaag cgcgtcatgc gggcgctgga catcgacgag
    10381 cagaaatggc ccgcccgcca ggcggtctgg ttcatcaacg cccagaagga tgaggggcgg
    10441 cgcgcaaagg acgtcgccgc gggtgacgat ctgtttcaga tcacccacaa gaagatctac
    10501 gagagctacg aaagcctttg cgaaagcggc ggcctagtgg attttgccga gctgcttctg
    10561 cgcagccatg agctgtggct caaccgctcg gatctcctcg cccactatca ggagcggttt
    10621 cagaacattc tcgtcgacga gtttcaggac accaacacga tacagtacgc gtggctgcgg
    10681 gtgctggcag gaagctcggc gaacatcatg gccgtgggcg acgacgatca gtcaatctat
    10741 ggctggcgag gcgcgcggat cgagaacatc catcagttct cccaggactt tgctggggca
    10801 tcgctgatcc ggctggagca gaactaccgc tcgacggcca ccatcctcaa agcggcgaac
    10861 gcgctcatac gtcagaacgt cgaccggctc ggcaaggaac tctggaccga aggcggggac
    10921 ggagaaccca ttcgcgtcta ttccgcctac aacgacctcg acgaagcgcg cttcgtggcc
    10981 gaacgcaccg ccgaagcgct ggcctcgggg gccagcgccg atgacgtcgc cgttctgtac
    11041 cgctcgaacg cccaatcccg ggtgctggaa gaagcattct tgagggcgca gattccttat
    11101 cgcatctacg gtggcgtgcg gttcttcgaa agggcggaga tcaagaacgc gcttgcctac
    11161 atgcgtctga ccgtggaccg gcattcggac acggccttcg agcgggttgt gaacaccccg
    11221 gctcgcggta tcggggacaa gaccgtggag gcgatccggg cccaggcgcg cgcccgcaac
    11281 gtgtccctgt ggcaggcgtg caaggaagcc cttgccgaga atgtgcttgc cagccgaacg
    11341 gcggccaaga tcgccgattt cctcgggctc atagacgggc tggcggagca gacggccgag
    11401 ctggatctgc acgaggtcgc cgacgcctgc attcagtcca gcggccttct ggagtaccac
    11461 atgcgcgagc ggggggagcg tggcctggct cgcaaggaga acctggagga gctggtcagc
    11521 gcctgccgcc agttcagcgg cgagcaggta ttccctctcc tggccgacgc cgccgagcag
    11581 acgtcgatac tcgaggagtt tctcgaccag gtagctctgg acgcgggtga tcgccaggcg
    11641 gaagcgggtc ctgcggtgca gatgatgacc ctgcactcgg ccaaggggct ggagttcccc
    11701 atggtatttc tcgcggggat ggaggaaggc ctcttccccc accgcatgtc cgccgaagag
    11761 cccgggcgca tggaagagga aaggcggctg tgctacgtag gcatcacccg cgccaagcag
    11821 acgctctacc tcacccatgc ggagactcgg cgcctgcacg gaacggatac ttacaaccgg
    11881 ccgtcgcgct tcctgctgga gattccgacg gagctcgttc acgaggtgcg catgggcggc
    11941 gcggtgcagc gaccctacgg ccggcctgca gaagccgggc acgcagtcgg ggctgagggc
    12001 ggcctgcgaa tgggtcagcg ggtgctacac gccaagttcg gcgagggcgt ggtgctccag
    12061 gccgagggtt ccggtgagcg cgcccgggtg caggtcaact tcgccggcgc cggcgcgaaa
    12121 tggttgatgc tgggctatgc aaacctgcag gccctcgatt gaaactggag aagagcgaca
    12181 tgatggtgat gcggatcctg gccctggtgc tcatgtttgg cctcgccggc tgcggcgcgc
    12241 cgcagcagac ggcgcccgaa gcggttcccg atcccgataa ggtctatcgc tggaagctca
    12301 ttacgacctg gccgaaaaac cttccggcgc tcggcatggc gccggagcgc ctggccgaaa
    12361 acctgcgggt catgagtcgg ggtcggctag acatccgggt ctacggcgcc ggggagctgg
    12421 tcggcgcgtt cgaggtgttc gatacggtac gccagggcac ggcggagatg ggccatggag
    12481 ccgcctacta ctggcgcggc aagctcccgg tagccgccat gttcgccacg gttcctttcg
    12541 gcatgatggc ccaggagatg aacggctggc tgcgctacgg cggcggcatg gagctgtggc
    12601 gcgaggccta cgaaccgttt ggcctcgtgc ccctcgccgc cggtaacagc ggcgtgcaga
    12661 tggcgggctg gttcaacaag gaaatcaact ccctggagga tcttcaggga ttgaagatgc
    12721 gcatcccggg gctcggcggc gaggtgctca agcgtgccgg gggcgtgccg gtgtccatgg
    12781 cggggtcgga gatttacacg gccatgcaga cgggcgtcat cgacgccacg gaatgggtgg
    12841 gcccctacaa cgacctggcc atggggcttc aggacgtggc acgctactac tactacccgg
    12901 gctggcacga gcccggcccc accctggaag ccatcgtcaa caaagcggcg tgggattcgt
    12961 tgcccgagga tctgcagaga atggtggagg tcgccgcggc tgccgccaac gaagatctcc
    13021 tgagcgagtt caccgcccgc aatgccagcg ccctcaaaac gcttctttcg aagcacaacg
    13081 tggagctcag gcgcttgccg gacgacgtgc tggcaaagct taaagtcgag tccgaggcgg
    13141 tggtggtcga ggctaccggt gactccgatc ttgcccgtcg tattcgtgaa tcctacgggc
    13201 ggtttctcag cgacgtgcgc gagtaccacg ccatcagcga gcaggcctac atcaatgccc
    13261 gctgagcctc tgcgcgaccg gccggcgggg aggtcgcgtt ggagctgaag gcgacggagt
    13321 actcggtcgc gtccggcatc gccaccgtgc gcctgaaccg gcctcatcgc ctcaacgcgt
    13381 ggacgggtcg aatgcacagc gagtaccgct ggtgcatcga tcgcgcggag cgtgacccag
    13441 cggtccgcgc gattgtcgtc acgggcaacg ggaggggttt ctgcgtcggg ggagacgccg
    13501 aagcgctgga agggcacgcg cgcaagggcg gctacgatcc gggaacgcct gcccggctcg
    13561 cagcgccggg atacggtcgg gcgcccgaat tcgacgccag ctttgcctat cactacgggc
    13621 tttccaaacc cgtgatcgca gccgtgaacg gcccggccgc gggggtcggt ctggcgctcg
    13681 cctgcttcgc agacctgcgt ttcgccgctg cgggcgtcaa gctcaccacc gcccacggca
    13741 agctgaacct ccccgcggag tacgggctgt cctggctgtt gccacggatg atcggcctca
    13801 cccgggcgaa cgacctgctg ctgtcgagcc gtgtcttcct cgccgaggaa gccctcgcca
    13861 tggggctcgt caacgccgtg tacccccagg cggagctcat ggacgaggtc tacgcttaca
    13921 ccagggagct catccgcacg gtgtccccgg cgtccctggc ccagacccgc tggcagatat
    13981 accgggacct gcatcgaagc gttgccgagt ccgtcagagc ctctgagcag ctgctcgatg
    14041 agatgatgcg gcaggaagac tatcgcgaag gggtggcggc gtttctggag aaacgcgccc
    14101 ccagctggtc cggatcagct ggagagactg gcgagtaatc gtttggctcg gggccgttcg
    14161 acccaggcgc cccgttgctc aggcgccatg cgggtcgaca accctccggc cgcgggccca
    14221 gacccccacc gggtttgcga gggcgtcgag gtcgtcgaga ggatttccgt ccaccaccag
    14281 aatgtcggcg gcaagacccg gcacgaggcg ccccgtcatc ccgtgaacgc ccagggctcg
    14341 agctgcggat gacgtcgcgg ttcgcaaagc ggcctggggg ctgagctcgg caatcagggc
    14401 gaagaccttc aatgccagtg gcaggtggtg atgaaacacc ccgggaattc cggcgtccgt
    14461 agaagcgatg aagggaattc ccagctccag catcgcccgg taggcggcac gaacgcggcc
    14521 gagcacccgc ccttcgccgc tgtccagcat gcgctgccag ccacgattga cggtgggcga
    14581 cacccagacg ccgcgctcga ggatcacgtt tgccaccggc tcctggaagt ccgatgccca
    14641 accttccgca ccggcccagg agcagtgctc gatggtggac acgccggcgc gggcggccaa
    14701 ctcgatgccc gtggtgccgt ggcagtgggc ggcgacgggc agcccgtgcg ccctggcggc
    14761 cgccgttatc tcgctcagcg tctccaggtc gaactgggcg gccagcggat cggatccgtt
    14821 ggtcatccgg ccgcccgtgg ccatgacttt gatcaggtcc acgccctggg tcacctgacg
    14881 ctccagcacg gacagggctt cttcggcgga gcccgcttcc ccgccccaga agtggcagtg
    14941 accgccggga cacgtgatgg gttgcccgga gcagagcagt cggggcccga tagcctggcc
    15001 ggccgccgtt ttgtcccgga tgccgagctc gagccaggca ccgccgccga ggtcgcgcgc
    15061 ggtggtaatt cctgcccgga ccatggccgc ggcccgttcc aacattctcg gcacctcgtc
    15121 cgggctcgtc gactcgggag gcttgtgatt gtcggggttg agctccaggt ggacgtgggc
    15181 gtcgatgagc ccagggagcg cggtggcccc ggggcagtgg acggccacgg cgcctttgct
    15241 cagctcccgg gcgtcgccga ccgccgcgat gcgtccattt tcgatgcgaa cggcatcggc
    15301 gtcgcaaaga gcctccccgt cccatacgcg gctcaccgta atggtggttg gccgctcgct
    15361 caccggtaga tgcgctccgg taaccaggtc gcacggctgg gccagatgaa gagcaggcac
    15421 aggagcagga tctgcagcaa cacgaacggg gccacgccgc ggtagatgtc ggctgtggaa
    15481 accgtggggg gagccacgcc gcgcagatag aagagcgcga agccgaacgg tggcgtcagg
    15541 aacgaggtct ggagattgac cgcgatcagc acccccagcc agatcgggtc tacacccatg
    15601 gccagaagca cggggccgac gatgggcacc acgacgaagg tgatttcgat gaagtcgagc
    15661 atgaagccga gcaggaagat caccagcatc acgatggcga gcgctcccca ggtgccgccc
    15721 ggcatatcgt tgaaccagct gtgaactagg tcgtcgccgc cgtatcccct gaagaccaga
    15781 gaaaacaagg aggcgccgac caggatgaag aaaaccatgg ccgtggtgtc aaacgtcgac
    15841 cggcacacct ggcgcaagac gccgacgtcc agcgcgcgat aagccgccgc caggatggtc
    15901 gctccggctg cgccgacacc ggcggcctcc gtcggggttg cgtagcccgc gaggatggac
    15961 cccagcacgg caacgatcag cagcatggga ggaagcagcc cgcccaagag ctgccaccct
    16021 gagccgtccg ggaccgcgtc cggaaccgcg tccgggaccg cgtccgggac cgagctggcg
    16081 gcgggcgcgg tgcccggccg caggaaggcg accagcatgg tgtaggccag atagagcccg
    16141 accaggatga gtccgggcac caccgcgcct gcaaacaggt ccccgaccga caccgtgtcc
    16201 ggggcgaaga tgccctgatt caactgggct tgctgatagg cgttcgagag aacctcgccc
    16261 agcagcacca gggcaatgga cggaggaatg atctggccca gcgttcccgt cgcgctgatg
    16321 gtgccgctag cgaaggccgg gtcgtagcga tcgcgaagca tggtgggtag agacatcagc
    16381 ccgagggtga ccaccgaggc gccgacgata ccggtgctcg cagccatgag catgccgacc
    16441 acgatgacgg caaaagccag ccccccgggc gttcccgcca tcagccgcga aaggctgctg
    16501 agcaggttct cggcgatccg cgtcttctcc atcatcacgc ccatgaagac gaacagcggc
    16561 accgcgatga gcgtggggtt cgtgatgatg ccgaacagcc ggccgggcag gaaacccaga
    16621 tcacggccgt cgaaggttcc gccggccatc ccgcccaggg cgaacagcag ggcaaccccg
    16681 gccagggtca gcgctacggg gtatccggcc agcagggcgg cgaagacggc caggaacatg
    16741 aagatcggta tgagctcgac cacgttgtca gcgctcgcta ccgaaatggg agggcagaat
    16801 gatgggcagc cggcgcacgg tctccgcgag gccctgcagc agcagggcaa ccgccatgac
    16861 cggaatgagc gttttcagga gaaagacggc cggaatgccg ccgacctcgg gtgatccctc
    16921 gagcacgagc cacgcgttgc gggcataggg aatgctgaaa tagaggatta gcaggcaggt
    16981 aggcacgagg aacagcaggt gcccggcaac gtcgatgatc ccccgggcgc gttcgctcaa
    17041 gcgcgagtga aggatgtcca ccctgacgtg gacgccgtct ctcaaggcgc agggaatgcc
    17101 gagcatgatc accgtggcgt gcagatagac gatggcttcc tgaatcggta tcgcgccctg
    17161 gtcgaacgcg tagcgcagaa cgacgacggt aaccatcagc acgaccatga gggcggttag
    17221 ccaggccacg ctgcgggccg tcgcctcggt gacgcgatcg gcgtgctcga gcagccgtgt
    17281 gttcagctca gccccccacc tggctggaag gcgccgcggg tcatggtttc ccatgcgacg
    17341 ccgacgaaca gcgaaaaacc gacccacacg ttgttggcga aggcgcggaa gcaaccctcc
    17401 cggcttcggt cccggatcag gtattgctga tagcagaaca agccggccgc gacggcgatg
    17461 ccccagaagt aggcgctgtg gtagccgagc cgctggccga ccaggaccag ggtgaggatc
    17521 gtgccggtct gcagaagccc gatcatcaga cggtcggcct cgccaaacag tatggccgtg
    17581 gacttgatcc ccactcgcag atcgtcctcc cggtccacca tggcgtacat ggtgtcgtag
    17641 gcgacgatcc agagaacgct tgccaggaaa agcagccagc cgggctccgg caccacgtgc
    17701 cgtaccgcgg taaacgccat gacgatgccc cagctgaacg cggcgcccag caccacctgc
    17761 ggaaggtagg tccatcgctt catgaaggga taggccaccg cgatggccag gcctgcgacg
    17821 gcgaaccatc gggtcagcgt gttgaggaag agcagcaggg tcagggcgag gaaggccagc
    17881 acgaagaagg cgcccagcgc ggcgttggtg ctgacggctc cggtcgcaag gggtcgatta
    17941 gcggttctgg cgacgccggg atcgaggtta cgatcggcgt agtcattcac cacgcagccg
    18001 gcggcccgca tcagaaacgt gccgacggca aagaccacga tgagatccac gggcggcagc
    18061 ccctcggcgg ctacccagag agcggccagg gtcggccaca ggagcagcag ggtgcccacg
    18121 ggccggtcga ggcgcatcag gcgcgcgtac gcgatcacgg cggagagcgg cggctgacgg
    18181 gttttctcca tgttcgcgcg ggcgggcttg agcttggcgg cggagtgtag cgtttcgcca
    18241 ccaggcaggc caggcggccg ccggccgcac gattgatttt tccgcggggg atgagtagtt
    18301 tatcgcgcct ttgcgccggc gccggcttga acaaagggaa gcgaccaaga atgcgaaaat
    18361 ggcaatgcat cgtctgcggc tggatctacg acgaagccga gggttatgag gaagagggca
    18421 ttgcgccggg tacggcttgg gaggatgtgc ccgaggattt cgtttgcccg gagtgcggcg
    18481 tcagcaaaga agacttcgag atgatcgagg tcggctgagg ccgattagaa cgtcgtgaac
    18541 cttacgatcc gcattctact cggcatgggc ttggggctcg tcctcggctc ttcgatccag
    18601 gcggcggaat tcgatccttc ccattgggtc cacgtctacc tcgtcggcgg cctgatagac
    18661 gctggcggcc agatattcgt tcgcagcctc cagctcatgg tggttcccct ggtattcgtg
    18721 tcgttggtgt gcggtgctgc cagcctgtcg gcgacggaca gcatcggtcg ggtggccgga
    18781 aagaccgttg ccctttatct gttcaccacg gccgtggcca tttccatcgc cctgctgctt
    18841 gcaacgatcg ttcagcctgg tgccggcgtc gcgccggcct ccctgggaac cgacttccag
    18901 ccggcggagc cgacgagcat caaggacacg ctggtctcca tcatcccgac gaacccggtt
    18961 agcgccatgg cggaaggcag gatgctgcag atcatcctgt tcgctcttct gctcggcctg
    19021 gccctgagcc ggtcggggca ggccggccgg cggctggccg gcgttttcga cgatctgaac
    19081 gaagcgttga tgaagctgat cacgctgctg atcaagctcg cgcccatcgg ggtcttctgc
    19141 ctgatggcga agctgttcgc caccctgggc tggtcggaga tcacgcggct cctgcagtat
    19201 ttcctgacgg tggtgggcgc tttgctcatt cacgtcaccc tggtctaccc gacgctgcta
    19261 atggtgctgg gcagggtcag cccgatccgt ttcttcggca agatgcgcga gccgctgctg
    19321 gtggccttca gcaccgcctc gagcggggcg accctgccgg tcaccctgcg caccgtggag
    19381 gagcgggttg gcgtgaaaaa cgaagtggca agcttcgtcg tgcccctggg cgcaaccatc
    19441 aacatggatg gaaccgccat catgcagggg gtggcgacgg tgttcatcgc gcagctgtac
    19501 ggcatcgagc tgggaatgtc gcagttcctc atcatcatcc tcacggcgac catggcctcc
    19561 gtcggcacgg cgggggtccc cggcgtcggc atgatcatgc tggtcatggt gctggagcag
    19621 gtcgggctgc ctctcgacgg catcgtcttc atccttgccg tggaccggct gctggacatg
    19681 ttgaggacgg cggtcaatgt ggccggtgac ggcatggtgg cgacagtggt ggcccggtcc
    19741 gaaaacgagc tggatctcga cacgttcgag gaccccgatg ccggccgtgt gatcggctca
    19801 taagagctgc ccggaagggc cctcctacac ggcgatgtgc ccgttgtcct cgggtgccca
    19861 ggcattgaga agctcccggg ccgttgtcgg gtccgtctgc aggaggcgtt cgccgcgtgc
    19921 cagcacctcg gcaatcagat ggctgtggcg ggcgagatcg gcaactcgga actgctgctc
    19981 gcccgtctgg cgcgcgccca gcagctcgcc cggtccgcgc agctccaggt ccttctcggc
    20041 gatgtgaaag ccgtcctggc tctcccgcat gaccgaaaga cgctggcgtg ccgccgcgct
    20101 cagcggtgtc ttgtagacca gcaggcatcg cgatgctttc tctccccgcc cgaccctgcc
    20161 gcgcagctga tgcagctgtg ccaagcccat gcgctccggg ttttcgatga ccatcagcgt
    20221 ggcgttcggc acgtctacgc cgacctcgat caccgtggtt gcgaccagca gccgcgtgcg
    20281 gccctccttg aagtccatca tggccttggc tttctgctcg ccggtcatcc gaccatgaag
    20341 caggcctatc gggggctcga ggcgctcggc gagcagctcg gcagtgggtt ccgcagcggc
    20401 agcgtcgagc tgctcggaat cctcgatgag ggggcatacc cagtagacct gctcaccccg
    20461 agccatcgca tcggctgcgc ttcgaatcac ttcctccttg cgggcgtcgc tgatcacacg
    20521 ggtgtcgatg ggcgtccgcc ccggcggaag ctcgtcgaga acggagacgt ccatgtccgc
    20581 gtacagggcc atggtcaacg tgcgcggaat cggcgtggcg gtcatgacca gctgatgggg
    20641 cgcggccccc ttgctgcgca gcgccatgcg ctggtgcacg ccgaaccgat gctgctcgtc
    20701 gacgatggtc agcgccaggg cgttgaagtt caccttgtcc tggaacaggg catgggtgcc
    20761 caccgccacc agcgcttcgc cctcctctag cgcctgctgc cttgcggcac gttctttcgc
    20821 cgactgggaa cccgtgagca acacgacggg gatcccgagc ggttcgagcc aggcggagaa
    20881 gttcacgtag tgctgctccg caaggatttc ggtcggcgcc atcaatgccg tctggcggcc
    20941 gtgctcggct gccctaatgg ctgcgaaagc ggcgaccacg gtcttgccgg agccgacgtc
    21001 gccttgcaga agccgcagca tgggggtttc gctggacagg tcctccagca cctccgtaat
    21061 cacccttcgt tgggcatggg tgagcctgaa tccgagcctg cgcaagagct cccggccgag
    21121 ctgctgggat tggggcagcg gcagcgcgcg ggatttggcg cgatcgcgct ggcgctgctt
    21181 catgaccagg tagtaggcgg tgagctcgtc cagggcgacc ctctcctggg ctcgttcgat
    21241 gtcggcggcg ggcgcgtccg ccggcggccg atgcagatag gcgagggtct ggtatggcgc
    21301 gccggagtcg ctgggccatt ccaaggaaag ggcctgatcg atcagcccgc gcaggcggtt
    21361 ctgccctaga cccttggtcg tggggtagac gggcgttagc tccggatctg gatccggggg
    21421 ctcggaggta aaggcgcggt actccgggtg cgccatctcc agcccgggac cgccgccccg
    21481 ggggccgaaa cggatctcgc cgaagcaacg cacgtacgtc cccggctgca gcccggactg
    21541 ttgacgcttt gagaagtgaa agaatctcag gcccaggtag ccggtgccgt cctcgatggt
    21601 caccagccag ctgcgacggc gaccgtagcc gagggatgaa tccaccacct gcccctgcac
    21661 caggcactcc tgttgtgggt ggacgtcgcg aagggggacc acgcggctgc gatcctggta
    21721 gcgaatgggc aggtgcagca gcaggtcgcc gacccggaaa atgcccaggc gctcgagctt
    21781 cgccttcaag ctcggtccga cacctttcag ggattccacg cgctgttcag cgatcacggc
    21841 gcctgccggc tcagggcttt gacgaatcag ccgtcaccgg ggtgagccat gacgccttcc
    21901 acctccacgg ccgcgccacg tggcagcgcg gccacgccga tcgcggcgcg cgcgggatac
    21961 ggctcgctga aataggctgt cattacctca ttgaccgact gaaagtcgtt gagatccgtg
    22021 aggtagacgg tgaccttcac cagatcgtcc agggacccgc ctgcagcgct ggcaacagcg
    22081 cgcagattcc tgaatacctg atgcgcctgc tcgatcacgt cgcccgcaac catgtccatg
    22141 gtcgctggaa ccagcggaat ctgaccggag agaaaaacga gactgcccgt tctcaccgcc
    22201 tgtgagtatg ggccgatggc ggctggggcg ttttccgtgc tgatggtttg ctttgtcatg
    22261 cggtttccgt tgtcacgcag gatgttggcc gaagacgggg gttattggct caccctgcct
    22321 atggagagca cgctgggaat ggcacgcatt tttcgcatga cccgggccag ggtgtcgcgg
    22381 tcggtgacgg acacgttgac gatcacgctg gtgacctcgg cgctccgctc ctccacggaa
    22441 atgttctcca cgccggcatc ggcgtcggct accgcggcag ccagctccgc gatgatgccc
    22501 ttcttgcggt tgacctgaat tcgcagggtg gtcagaaact cgttgtcggg tgagttggcc
    22561 cagtacgcgg gaatgagctc cctgttgccg cgtcgacggc gcacctcctg catgttgggg
    22621 caggaggcca tgtgaactac gaaccccttc cccggtgtca tgtgcccgac gatgggatct
    22681 ccgggcacgg ggccgcagca gcggccgtag gtgatgacca ggccctcccc gccgccgatg
    22741 gcaaccgggc cgccggtctc taccggcact ccctgaaagt caggatcgtc cgcggcgaga
    22801 aggcgctggg cgacgaggta ggccatgagc tcgccgctgc cgatcctcgc cagcacctcg
    22861 tcgagccgac gcacgccgaa ttccgtgaaa acgcgacgca ggcgccggaa gtcgaggtcg
    22921 ttgatgctct tgttggcatg ggcgagcgag cggttgagca ggcgtcggcc gaacgcgatg
    22981 ctgtccgttg cctgctgggc cttgagcgcc tgacggatac cgctgcgggc cttgctggac
    23041 accacgaagg ttagccagtc ggggttgggt cgcgcgcctt cggaagtgat gatctccacg
    23101 ctctggccgc tctgcagctg ctgtgagaga ggcgccagat tgcggtcgat gcgacaggcc
    23161 acgcagcggt tgccgatgtc cgtgtgaacc gcgtaggcga aatccacggc gcaggcgccg
    23221 cggggcagct cgagaatgtc gccctggggc gtgaacacgt acacctcgtc agggaacagg
    23281 tcgatcttga ggctttccac gaattccatc gggttgcccg cacgctgctg cagatcgagc
    23341 agatccttga cccagcgccg cgcccgctgc tggctgctct ggaaatcctc cccttcggac
    23401 ttgtacagcc agtggccggc gatgccgttg tcggcgacgg ccgccatgtc ccgcgtgcga
    23461 atctgcacct cgatgggaac gccgtgcatg ccgaagagcg tggtgtgcag cgactggtaa
    23521 ccgttcacct tcgggatcgc gatgtagtcc ttgaagcggc cggcaaccgg cttgtaggtg
    23581 ttgtgcacca ccccgagggc ccgatagcag tcctctacct cgtccacgat cacgcggaac
    23641 ccgaacacgt ccatgatctc ggcgaacggc ttgtgctgcg ccttcatctt ccggtagatg
    23701 gaatagaggt gcttctcgcg accgattacc tccgctgtga ccgattcctg gtccagcgcg
    23761 tcctggaggg atttacgcag ctcttcgacc agctctttcc ggttcccccg ggcggcacga
    23821 acagcccggc cgattcggtc ggcgcgcagc gggtacaggg cccggaaacc aagctcctcg
    23881 aactccacct tcatggtgtg tatcccgagc cggttggcga tgggcgcgta gaaatccaaa
    23941 gtctcccgcg cgatgcgctg acgcgcctcc cgggacatga cgccgatggt gcgcatgttg
    24001 tgcagcctgt cggccagctt caccatgatc acccggatgt ccttggccat ggccagagcc
    24061 atcttctgaa agttttccgc ctgggcctcg gctcgggagc ggaatatctt tgacagcttt
    24121 gacaccccgt cgacgatctc cgccaccgac ttcccgaaac gctgggccag ggcgttgcgg
    24181 tccgcgtcgg tgtcctcgat gacgtcgtgg agcagagcgg ccatgatcgt ctcgtgatcc
    24241 atgcgcatcg tggcgaggat gttcgctacc gccagggggt gcgagatgta agggtgcccc
    24301 gtgcggcgga actgcccttc gtgggcgttg atggcgtagg cgtgggcgcg ctcgacgcgg
    24361 cgaacctgat ctggctccag ataagtggac agcagggtcg tcagggattc gatcgatacc
    24421 ggcgcggcga tgtcgtcgcg cctcgactcg tcggcgagca ggccgacgaa gtaggattcg
    24481 ggttggggga gcttcggttt ttcgccagcg cgtgttttct tgcggacaac gcgagacccg
    24541 gggtgcttgg agccctggtg gtcagatctc gccggtttcg ccatctccta taccgaaaga
    24601 aacctcaagt tcctcatcct ccgtggaggc ttcttgtgcg tcaagcattt catttgatat
    24661 gagcccttcg gcaatttcgc gcagagcgat cacggtgggc ttgtcatttt cttccggaac
    24721 caggggctcc gcgccgaagc ggcgcatctg gcgtgcccgc cgcgttgcga gcatcacgag
    24781 ctcgaagcgg ttttctacgt tttccaggca atcctcgacg gtgatgcggg ccatggcgaa
    24841 cctctgatgc gatcttctaa agtggctccg agagagccgg tgtgcagcga gcgcgcgagt
    24901 gtagccgccc ttccggaaga ttgtaaagtg ccgtcactcc tgccggagga ggtctttgag
    24961 cagcgcgccc agccgctccc gctggggctg cagcccgagc cggctcgccg cgatgatctg
    25021 cgtcagctcg ctcagggctg tggcgaagtc gtcgttcacc accaggtagt cgtactcccc
    25081 gtagtgcgaa atctcctccc gggctttgtc gctgcgcgcc tcgatgacgt tggcggcatc
    25141 caggccccgc ccctccagcc gctgcaccag ggcggcgcgg ctgggcggca ggatgaaaat
    25201 gctggtcgcg tccgggtaag cggcgcgaac ctgccgggca ccctgccagt cgatctcgag
    25261 aatgacgtcg acgcctgtgc gcagctgctc gtcgacggtc cttttcgacg ttccgtagcg
    25321 gtttccgaag acgtcggcgt cctccaggaa atcccctcgt tgcgccatct cccggaacaa
    25381 tttcggttcg acgaagtggt aatccacgcc ttcctgatcg cccggcctgc gcggacgcgt
    25441 ggtgtgggag atcgaaacgg ccaggttgcc gtcctgagcg atgagcgcac gaacgaggct
    25501 cgtcttgcct gttccagagg ctgcggaaac gatgaacagc aggccctggc gagacgcttg
    25561 gctcattcga cgttctgaac ctgctcgcgg atctgctcga tggcgacctt caggtccacc
    25621 gccttctgcg acgtctgggc cagcaccgac ttcgcgccca gcgtattggc ttcccggtta
    25681 agctcctggg tgagaaagtc gagccggcgc ccgtggggcc ccgggccgcg caggttgctg
    25741 cgggcctcct ccacgtgaat gaccagccgg tcgagctcct cggcgacgtc ggcccgctgg
    25801 gccagcaggg cgacttcctg gtccaaccgg ccttcgtcgg cctgaacctt cagatcgttg
    25861 agtcgttggg tcagcctgga gcgcacctcg tgactcatcg tcgcggtcag cgctttgacc
    25921 tcgcgagcga tgctgtcgat ctcgtccagc cgctcggaga tgacggaggc gagctgcgct
    25981 ccctcccgtt gccgattgtc gatgagctgg acgagcgctt cctcgaacag atcagtagcg
    26041 gcctggacga ggggttcgtc ctccagagtt gacgcatcgc caatgatgcc cggccagcgc
    26101 aggacgtcca tggcgttgcc gggccctatc tccggagcgt cccggcggat ctgctcgacc
    26161 gcggcgagca gttggaggag cagcgggcgg ttgagctcga taccgtcgcc gccggtgctt
    26221 cggtcgatct tcagcgagca gtcgaacttg ccgcgcttga cgtgacgccg cagggtttca
    26281 cgcaggccgt gctccaacgg gcgcatctgc tcgggaagcc ggaactgagt ttccagaaaa
    26341 cggtgattca ctgatcgcat ctcccagatc agcgagagcc ccttggcgct gccctggacg
    26401 cgggcgaatg cggtcatgct gtggatcatc ggtaggtcag cggcacgtaa aatggcgagc
    26461 gcgcattcta cccgggcggg ggccctgcca tccaaccgcg ccgcgcgatc gcgttgctgc
    26521 cggccgttgc ctttataatg cccgcccact tctgccgtcc cgaggatttt gatgaccgaa
    26581 acacaggttc agcgtcccag tggccgggcg ccctcagaga tgcgggaagt ccgcttcacc
    26641 cgcggattca ccaagcatgc cgcgggctcc gtcctcgtgg agttcggtga taccaaggtg
    26701 atctgtacgg ccagcgtgga gaactcggtg cccggcttcc tgcgaggcaa gggcgtgggc
    26761 tgggtgactg ccgagtacgg catgctcccc ggcgccaccc atgtgcgaac cgatcgagag
    26821 gcagcgcgtg gcaagcaggg cggtcgcacg caggaaatac agcggctcgt cgggcgcgcc
    26881 ttgcgggcgt ccgtggacat gcaggcgctc ggcgagcgca ccatccggct ggattgcgac
    26941 gtgatccagg cagacggcgg cacgcggacg gcatccataa cgggctccgc cctggcgctg
    27001 gccgacgcca tcgcctcggc cggttgcaac ggggcgctca aggagctggt tgcctcggtg
    27061 tcggttggtc tgtggcgcgg tcaacccgtg ctggatctgg attacgccga ggactccacg
    27121 gcggagaccg acatgaatgt ggtgatgctg gaatccggtg ggttcatcga gattcaggga
    27181 acggccgagg cgggggcgtt cagcgacagc gagctgatga gcatgctggc cctggctcgg
    27241 tcgggcacgg cggagctgct gcgccgccaa cgacaggcgc tgagctcttg agcgcctccc
    27301 ggaacgatcg ctccctggtg gcgtttctca tcgaacaggg cgttctcgcg ttcggggatt
    27361 tcacgctgaa atccggccgc agaagcccct acttcttcaa cctgggggct ctgaatacgg
    27421 gttcagccct ggcgcggctg ggcgccgcct acgccgagcg cattcgccgg ctggacgtgc
    27481 cgttcgacgt ggtgttcggt cccgcttaca agggcattcc cattgcggtt tccactgcgg
    27541 aggcgcttgc tgctcttggt ggcgacgttg gttgggcatt caacaggaag gaagccaagt
    27601 cccatgggga gggcggcctc ttcgtaggcg cagacgtctc gggcagggtc atgctcgtgg
    27661 acgacgtgct taccgcgggt accgccgtgc gggaagcggc cgcgctcatt ggatctgcag
    27721 gcgcggagat tgccggcgtt gtcattgcgc ttgacagaca ggagcgcgta gccccgaact
    27781 caggggaaac ggcggtttct gccctggagc tggagcttgg cgcaccggta gtcagcattc
    27841 tcaagctcca agacgtgatc gagtaccttg atctggacgt cgatcgggac aaacatcgag
    27901 ccgaactaat ccgcgaaatg aagcggtacc gcgacgagca ttgcgttctg aactgaaccg
    27961 gctagtcgct cataactcga cgattgagcg attgatggga acacgatacc gaggcaattt
    28021 ccgatgtcgc ccgcgggcgg cggtcttgat catggtggcg atcctcctga tgccgtcctg
    28081 ggcttcggcc aatcgtctgt accggtacct cgatgccagc ggtcacatag cgatctctca
    28141 caccatcccc agcgaccggg tcaagttcgg ctacgagatc gtcgacgccg gcagcgggcg
    28201 ggtgatcgag gtcgtggagg cccagaagag ccctgaggaa atcgccaggc tggaacagga
    28261 agcggccgcc cgcaaggcgt gcgaagacgc gctcgagcgc gtctattcgg tgtaccagtc
    28321 tgagctcgat atcgagaacg cagaagagca gatgctggaa tctctagagc agcgcattgc
    28381 gaacgccgag ttcaacctcg agcgcgcgca aagccagaaa gatggcttcg aggccaccgc
    28441 tgcccgactc gagcgctccg gcaaggaact ggacacctcc ctcatcgcca atatcgagag
    28501 gtcccaggtt caggtgagga gcctggagac ggagatcgtt cagcgccgcc atgagcaggc
    28561 gaaagccaag gaacgtttcg cccgtgatcg ggctcttttc cgccaggaaa tctgtgtcga
    28621 gcagacggcc atcagcgcgg ttacggaaac gaactgaggg cgtttgccgt tccttggctg
    28681 gctagccgaa gacggcccct tccagagcct tccactgttc ctcccagccg gaggtcggca
    28741 gcgcgcgaaa ttcgcttcga acgaactggc tgatgcgacc ctccgcactg gccaggagca
    28801 ggttggcggc cgtcgtggca ccgagcgttg gtgccggcac ccgggcggcc gaccagtccc
    28861 ggatgatcga ccgaagctcg gtttccagcc gctcgaaaac ctggcgcatg cgcgcccgaa
    28921 gccgttgggt ctcgccctgc agggcgtccc cgaccagcaa gcgcgccagt cccggattcc
    28981 gctcggcgaa ggaaagcagc agccacagca cggcccggca gcgggacgcg gcgctggcct
    29041 cctctgcgag aatgcgattg atgcggctga agaggctctc ctcgatgaac tcgatgagcc
    29101 cctcgatcat ttttgccttg ctgggaaagt gccggtagag ggcggcctcg gataccccga
    29161 tctggccggc cagggcggcg gtggttatcc gcgttcccgg gttgctttcc agcattcgcg
    29221 cgaacgcgct gagaatttcg tcgcggcggc ccgatctagg aggcatcgga cccgctcgcc
    29281 tcggcggtgg tgatctgcgt accgattccg gcatcggtga agatttccag caggagagcg
    29341 tgaggtatgg ttccgtcgat gatctgtgtg ctccctacgc cgcgctcggc agcggacacg
    29401 gcgcactgcg ccttgggcag catgcccgag ccgatggtgc ccgcggcgat gagctcgttg
    29461 atctccgcga cgctcagcga ggtgagtgtc tggccattgg cgtccaggat gccggcggtg
    29521 ttggtcagca gtatcaactt ttctgcgcgc agctcctccg ccaggcggcc tgcgacgagg
    29581 tcggcattga tgttgtagga ctcgccatcg ggccccacgc caatgggcgc gatcaccggg
    29641 atgaagttgc tgcgcacgag ggcgtccagg acggccgggt tgacgctttc gacctcgccg
    29701 acgtggccga tgtcgatgat ctccgacggg gcgagctccg ccgcgtcggc gctcagcgcc
    29761 agccggtgcg ccctgatcag atcgccgtct ttacccgtta tccccacggc gcgaccgccg
    29821 ttcttgttga tcagggatac gatgtcctga ttcaccagcc cgccgagaac catctccacc
    29881 acgtccatgg tctcggcgtc cgtgacccgc atgcctttga cgaagcgggt gggaatgttg
    29941 agccgatcca ggagctcgcc gatctgcggc ccgccgccat ggacgatgat gggattcaga
    30001 ccgaccagct tcagaagcac gacgtcgcgc gcgaaggtcg ttttgagcgt ctcgtccacc
    30061 atggcgttgc cgccgtactt gaccaccacg gtgccgccgt ggaatcgctg tatataagga
    30121 agcgcctcgg tcagtacgct cgcgatgttg cgggcttggg attcggagat cggcatggct
    30181 tcaggagatc ttcagcgagg ggtcgacctt tgcgatctgc accgcgaaat ccgactgaat
    30241 gcgcgcgagc gcctcggcgt cgtcggcctc gaaacgtagc gtgagcacgg ggctcgtgtt
    30301 cgaggctcgg atcagccccc agccgtcggg gtagtcgacg cgcaccccgt caatgctggt
    30361 gaccgtgccg tcgccgaaat cggccgacct gctgagctcc tcgatgatcc cgaacttcgc
    30421 atcctccgtg gtctggatct tgagctcggg cgtgctttgc cccgtgggga actgctcgaa
    30481 cacctcgtcc acggtgccgc cctcggcgcc gaggatttcc agcagccgtg ccgcggagta
    30541 gagggcgtcg tcgaacccgt accaccgatc gttgaagcag atgtgaccgc tgaactctcc
    30601 gcccagcagc gctccggtct ccttgagctt cgccttgatg tgggagtgtc cggtcttcca
    30661 catgatgggc cgcccgccgt ggtctccgat gatggcgttc agatgccggc tgcacttcac
    30721 gtcgtagacg atgtcggcac ccggattccg gctcacgata tcctgggcga acagcatcag
    30781 cagcctgtcc ggccagatga tctggccgga agaggtcaca actcccaggc gatcgccgtc
    30841 gccgtcgaag gcgagtccca ggtcggcgga ttcggcctgg accacggtga tcaggtcttc
    30901 caggttcttt gggtccgccg gatccggatg gtggttgggg aaatcgccgt tgacgtcgca
    30961 gtaaagcggg acgacctcgc agccggcttc ctccagaagc acgggcgcga cggcacccgc
    31021 cacgccgttg ccgcagtcga cgacgacctt gagctgctgg gcgaccacga tgtcgtccag
    31081 cgcgctgtct atgtagtagc ggacgatgtc gacttcttcc acgtcgccct cgccctcaga
    31141 cagctcgttg ttctccagcc gctccttgag ccgctggatg cgttcttccg cgagggtgtc
    31201 cccagcgatc atcatcttca ggccgttgta ctcgggtggg ttgtggctgc ccgtgatcat
    31261 gatgcccgtg cccaggtcca gagcgtgggt ggcaaagtag agcagcgggg tcggcacctc
    31321 gccgacatcg gcgacgtcga gaccgccatc ggtcaggccc ttgatgagcg cggtgcgcag
    31381 cgcctcgctc gagtgtcggc catcgcggcc tacggccgcc cgggtctgct cctgggccag
    31441 cgcctcggcg gcaaacgccc gcccgatcca gtacacgacg tcctcggtca gattatcggt
    31501 gacgatgccg cgaatgtcgt aggcccggaa tatctgcggg tccagatcga tccccatctc
    31561 gcccagagac acttcctcgc tgacctcgat gccgaagttg tccttggcgt cgtcggtaaa
    31621 gccgcggctg ggcagcgtgc ttgccgctcc cgctcccggc tcggcctcct cgccgacggg
    31681 ctcggcttcc tcctcgacgg ccgtctcctc cgctggcgct tcctcgggcg gttgggcctg
    31741 caccggcatg gcggcggcgg cctggcgcag acgcttggta acgcgcgtgg tggagaggta
    31801 tccgccggcg attaccagca gaagggcgac cccgaatggc agtgccaggt cgaccatctt
    31861 ggtcaccggc tgcagaatcg ccgaaccggg ctcaaagacg agattccatt tctggttctc
    31921 cagcgtgacg atctccgcgg cggcggggtc ggattggccg ccccagccga aaatctcgat
    31981 ggggtcgacg ctctcggaga gctgctcgat gcgcacgcgc ccgatgcgct catccagcca
    32041 ggccagcccg ctcaggaaaa agtccggaga gaccacggcg aacaacaccc cggcgcggga
    32101 gcccggctct ccaatctgcg cagccatgta gacataaccg cgctgattga tcgatgcgcc
    32161 ctcgggcccc accat
//
LOCUS       DBNHLM010000030         5417 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_900703, whole genome shotgun sequence.
ACCESSION   DBNHLM010000030 DBNHLM010000000
VERSION     DBNHLM010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_900703"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3488)
                     /locus_tag="AC16QF_01465"
     CDS             complement(<1..3488)
                     /locus_tag="AC16QF_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HIR6447248.1"
                     /translation="MVTLLLLSGFLTACGGGGGSRSSESSTQTPQTSEPPPSPALSLA
                     ASKSVVVAGSAVTLSWSSEDADQCNASGDWSGTKSLSGSESVGPVNDDVTFVLDCSGE
                     GGAVEKAVSVNVAPPTVALNVSEPVVTMGAAVTLTWSSTGATSCEASGGWSGTKDASG
                     SETVGPVEADSEYALSCTGPGGGIDASASVTVAPPTLELNQSESLVMKNAVVTLTWST
                     TSATECEASGDWTGTKAANGSETVGPVQTDSQYLLNCTGPGGMIEETGLVSVAVPPKV
                     ELNASSTFVDPGSPVTLSWNAANADACSASGDWTGQRAVSGSEVVGPLEDTERFSISC
                     TGPFGTSSADVSVVVTGKTLVWQAPSENLDGTPLQDLAGFKVHWGERSRQYTGSRLID
                     SPTTTSWEPDIEPGTYYFAVTAIDEDGTSSWYSNEVVKGVRSAGIPEISLELSDTVVD
                     AGAFVTLSWTARQVESCDASGAWVGAREAAGVEVVGPLVADQDFTLTCTGPAGTVTRR
                     VEVDVTMEEGSPALAYAAVPGVALSLEASRHLVAEGDRVSVSWVASNADACTASGGWA
                     GERPPAGAFESEPLAASTVFSLSCSGPGGNVVREVSVDVVPAPSASLTVSETLVVAGE
                     STTLTWSSENAASCEAFGDWSGVRSVSGSADTGPLLEDSSFGLICSGPLDRAYASVEV
                     RVDRAARAHWTFDEGAGDVLADSRGSSDGQIVGAVWAQGVSGGALDFNGDGDHVAVDF
                     AAALDVGGTEITLAAWILPRDRSLGRSSAIIARPAALGDSHVYGLLARDGKLVFRLDG
                     EDMISGSTLELGQWQHVIVVYNGAVKRIFVNGVAEPTLQEKTDAIDSNGEGAAIGGDA
                     DGTSGFNGLIDEVVLFDEAIGADQAQRLYASFAPPLILSVFEDVSVDAGFTGPGANGQ
                     AAAFADANADGLVDLYLTLGSDPEQAVLQDRYFHNYDGTFVEEALTRAIEDADGGSYG
                     AVWADLDNDGDYDLVNASAYTDLGEAGGVAANNNVYENDGTGNFLDVTALGAVADAAN
                     ARRSRGVTAHDADGDHDLDLFSIPLAADAGSNEAYLNDGSMVFSANLNAVLTTTPASR
                     GLIDTDFDGDGDVDILAASSPGAFAILRNDGAGTFVSVAPSDPTLGIEDDAEDGISAA
                     DVDVDGDLDLLLVSDGS"
     gene            3913..4668
                     /locus_tag="AC16QF_01470"
     CDS             3913..4668
                     /locus_tag="AC16QF_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIR6447249.1"
                     /translation="MSNRTVTACVLIIGNEILSGRTQDTNLNHLATTLGGWGIQVREA
                     RVIPDIESVIVDAVNTARAAFDYVFTTGGIGPTHDDITAESIAKAFGVGLVTSPEIAA
                     IIEQRPAPPDVMAARMRMARIPDGAKLVDNLTGGPQGFCMDNVYVMAGIPLVMQAMLK
                     SLEGKLRCGPVVRSVSVTAYLGESQIATELGEIQARHPDVDIGSYPFFRNDRYGTSLV
                     IRGTDERELEDVLAKVEAAIVSEGETPQDVHRA"
     gene            4784..>5417
                     /locus_tag="AC16QF_01475"
     CDS             4784..>5417
                     /locus_tag="AC16QF_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6447250.1"
                     /translation="MNPSGKKAVVLGGTSGIGLATARQLMEAGAHVVVVSRSDSNAAG
                     ARETLGDSADIRQLDVLDRKRMAGLFEQLAPFDILVNAATGGERATGPFLQMDLDGFQ
                     GSFRKLWGYVNSVRLGAEHMAEDGCITLVSGSPARKCPPGFAAISSVGNAVEGFVRAV
                     AAEIAPRRINSVSPGFIDTPMFPVEGEAREKFLAQATAGNLIKRPGTAEEV"
BASE COUNT          988 a         1865 c         1694 g          870 t
ORIGIN      
        1 gacccatcgg atacgagcag cagatccaga tcaccgtcga cgtccacgtc ggcggccgag
       61 atgccgtcct ccgcatcgtc ctctattccg agggtcgggt cggacggcgc cacgctgacg
      121 aacgtaccgg caccgtcgtt gcggaggatg gcgaacgcgc ccggggaact cgccgccaag
      181 atgtcgacgt ctccgtcccc gtcgaaatcc gtgtcgatca acccgcggct ggccggcgtg
      241 gtcgtcagta ccgcgttcag gttcgccgaa aaaaccatgg aaccgtcgtt caggtaggcc
      301 tcgttgcttc ccgcatccgc cgccaggggg atgctgaaca gatccaggtc atggtctccg
      361 tccgcgtcgt gggcggtgac gccgcgcgag cgtctggcgt tcgcggcgtc ggcgacggca
      421 cccaaggcgg tgacgtccag gaaattcccg gtgccgtcgt tctcatagac gttgttgttg
      481 gcggcaaccc cgccggcctc accgagatcc gtgtacgcgc tcgcattgac aagatcgtaa
      541 tcgccgtcgt tgtcgagatc tgcccagacc gccccgtagc tgccaccgtc ggcgtcctcg
      601 atggcgcggg tgagagcctc ctcgacgaac gtaccgtcat agttgtggaa gtaccggtcc
      661 tgtagaaccg cctgctcggg atccgagcct aacgtcaggt acaggtccac caggccatcc
      721 gcattggcat cggcaaacgc tgcggcctgg ccgttggccc cggggcccgt gaagcccgca
      781 tccaccgaca catcctcgaa gaccgagagg atcaacgggg gtgcgaagga agcgtatagc
      841 cgttgggcct ggtccgcgcc gatggcctcg tcgaagagca ccacctcgtc gatcaggcca
      901 ttgaagccgg acgtgccatc cgcatccccg ccgatggcgg caccttcgcc gttggagtcg
      961 atggcgtccg ttttctcctg caacgtgggc tccgccacgc cgttgacgaa aatgcgcttg
     1021 acggccccgt tgtagacgac gatgacgtgc tgccactgcc ccagttcgag ggtgcttccc
     1081 gagatcatgt cctcgccatc taggcggaag accagcttgc cgtcccgagc cagcaggccg
     1141 tagacgtgcg agtcacccag ggcagcgggt ctcgcgatga tcgcggagct gcggcccaga
     1201 gagcggtcgc gcggcaggat ccaggcagcc agggtgatct cggtaccccc tacatccaag
     1261 gccgccgcga agtcgacggc aacatgatca ccgtcgccgt tgaaatcaag ggcaccgccg
     1321 ctaaccccct gcgcccagac ggcgccaacg atctgcccgt cgctgctgcc cctggaatcg
     1381 gcgagcacgt cacccgcgcc ttcgtcgaac gtccagtggg cccgagcggc ccggtcaacg
     1441 cggacctcca cgctggcata ggcgcggtca agaggcccgg agcagatcag gccaaagctt
     1501 gagtcttcga gcagcgggcc cgtgtccgcc gatccggata cggagcgtac ccccgaccag
     1561 tcgccgaacg cttcgcagct tgcagcattt tccgacgacc acgtcagggt ggtggactcc
     1621 ccggcgacga ccagggtttc agagacggtc agcgaagcgg acggagcggg aaccacatcc
     1681 accgagactt cccgcacgac gttgccccct ggtcccgagc agctcaggga gaagacggtc
     1741 gacgccgcaa gcggctcgga ctcgaaagcg ccggccggcg gtcgttcacc cgcccaccct
     1801 cctgacgccg tgcaggcatc ggcattggat gccacccagg agacgctgac ccgatcaccc
     1861 tccgcgacga gatggcggga cgcctccaga gacagggcga cgccggggac cgccgcgtac
     1921 gccagggccg gtgagccttc ttccatggtg acgtccacct cgacgcgacg ggtcacggtg
     1981 ccggccggcc ccgtgcaggt cagggtaaag tcctgatcgg cgaccagggg tcccacgacc
     2041 tctacgcccg ccgcctcgcg ggcaccgacc caggcgcccg acgcgtcaca agactccacc
     2101 tgccgggccg tccagctgag cgtgacgaac gcacccgcat cgacgacggt atcggaaagc
     2161 tcgagagaga tttccggaat tcccgcggag cggacgccct tgaccacctc gttggaatac
     2221 cagctcgaag taccgtcctc atcgatcgcg gtgaccgcga aatagtaggt gcccggctcg
     2281 atgtcaggct cccaggaggt cgtggtcggc gagtcgatca gccgagaacc cgtgtactgc
     2341 cggctgcgtt ccccccagtg aactttgaag cctgccaggt cctgaagggg ggtgccgtcg
     2401 aggttttccg aaggcgcctg ccatacgagc gttttgcccg tcaccaccac gcttacgtcc
     2461 gcgctggacg tgccaaaggg cccggtgcag gaaattgaaa atcgttcagt gtcttccagc
     2521 ggtcccacca cctcgctgcc cgagacggcg cgctgcccag tccagtcacc cgacgcggag
     2581 caggcgtctg cattcgcggc gttccagctt agggtgaccg ggctgcccgg atcgacgaac
     2641 gtgctcgatg cgttgagctc caccttcggc ggcacggcta cgctcacgag ccctgtttcc
     2701 tcgatcatcc cgccggggcc cgtgcagttg agcaggtact gcgaatccgt ctgcacgggg
     2761 cccaccgtct ctgaaccatt cgccgccttg gtccccgtcc agtcacccga cgcctcgcac
     2821 tcggtggcgg acgtggtcga ccacgtcaac gtcaccaccg cgttcttcat gacgagactt
     2881 tccgactggt tcagctccag cgtcggcggc gccaccgtca ccgaagccga tgcatcgata
     2941 ccacccccgg gacccgtgca gctcagcgcg tactccgagt ccgcctcaac cggacccacc
     3001 gtctcggatc cggacgcgtc cttcgtcccc gaccagccac ccgatgcctc acagctcgtc
     3061 gcccccgtgc tgctccaggt cagggttacc gcggcgccca tcgtcaccac cggctccgat
     3121 acgttcagcg ccaccgtcgg cggcgcgaca ttgacggaca ccgccttctc gacggcaccg
     3181 ccctcgccgc tgcagtccag gacgaaggtg acgtcgtcgt tgaccggacc caccgactcg
     3241 ctgcccgaga ggctcttggt gccggaccag tcgccggaag cgttgcactg atcggcgtct
     3301 tccgatgacc aggacagcgt cacggcgctg cccgccacaa ccacggactt ggatgccgcc
     3361 agagaaaggg cgggggaagg cggcggttcg ctggtctggg gcgtctgggt gctgctttcg
     3421 gacgaacggg aaccccctcc cccaccacag gcggtgagga agcccgacag caaaagcaat
     3481 gtgaccatcc gcatcgaaat catgggatgg ctagaccgat cgcatccagt gcccccttgg
     3541 atgtgagcca aatgtgtccc gaggtaatca gtatatgtgg accagcacag gaattcggcc
     3601 cttccgacac gcccgagccg tcatctatgt aaatgataag taaggaaaaa gtttgctctc
     3661 agcgtgtcgc tccccaggta gacgctcggg gattcacaac taatttcaat ccattcaagg
     3721 ggttatatat gtcatatagg ctgacattaa aactatctca tatcgacttt acaagcgggc
     3781 ccttccctcg gtgccccgcc gctcgaggca acctgtctga ccaaagggcg gcggactgcc
     3841 ggaaagtgtt aggatgcgct ccgcccgggc ggggtgcccg gagaaccgaa cagctgaaac
     3901 ggaacccctc ccatgagcaa ccgtacggtc acggcctgtg tgctgatcat cggcaacgag
     3961 atcctctccg gacgcactca ggacaccaat ctgaatcacc tggcaacgac cttggggggt
     4021 tggggcattc aggtacgcga ggcccgcgtc attccggaca tcgagtcggt catcgtggac
     4081 gccgtgaaca cggcgcgagc ggccttcgac tacgttttca ccaccggcgg catcgggccc
     4141 acccacgacg acatcacggc cgaatccatc gccaaggcat tcggcgtggg cctcgtcacg
     4201 tcaccggaga tcgccgccat catcgagcag cgccccgcgc cgccggacgt gatggccgcc
     4261 cggatgcgca tggcacgcat ccccgacggc gccaagctgg tggacaacct gaccggcggg
     4321 ccccaggggt tctgcatgga caacgtctac gtcatggccg gcatacccct ggtcatgcag
     4381 gcgatgctga agagcctgga aggcaagctg cgctgcgggc ccgtggtgcg ctccgtctcc
     4441 gtgaccgcct atctgggcga gagccagatc gccacggagc tgggcgagat tcaggcgcgc
     4501 caccccgacg tggacatcgg cagctacccg ttcttccgaa acgaccggta cggcacctcg
     4561 ctggtgatcc gcggaaccga cgagcgtgag ctggaggacg ttctcgcgaa ggtcgaggcc
     4621 gccatcgtct cggaaggcga gacgcctcag gacgtgcacc gggcttgagc ccgcccgctc
     4681 cttccgcgcc aggacgcctg cggccaggtg ccggcgccag agcgacttcg tccgcggtat
     4741 cgcgcgcgaa aacaggatta gaaacacaag gagacagttg aagatgaacc cgtcaggaaa
     4801 gaaagccgtc gtgctcggcg gcacgtcggg catcgggctc gcgacagctc gccagctgat
     4861 ggaagccggc gcccacgtgg tggtcgtcag ccgatctgac agcaacgccg ccggcgcccg
     4921 ggaaaccctg ggggactcgg ccgacatccg tcagctggac gtcctggacc ggaagcgcat
     4981 ggccgggctg ttcgagcaac tcgcgccgtt cgacattctc gtcaacgcgg caaccggcgg
     5041 cgaacgcgcc accgggccgt tcctgcagat ggacctggac gggttccagg gctccttccg
     5101 caagctgtgg ggctacgtca actccgttcg gctcggcgcc gagcacatgg ccgaggacgg
     5161 ctgcattacc ctggtgagcg gctcgccggc acgaaagtgc ccccccggct ttgccgcgat
     5221 atccagcgtc ggcaacgccg tcgaggggtt cgtacgagcc gtcgccgccg agatcgctcc
     5281 gcgacggatc aactcggtgt cacccggctt cattgacacg cccatgtttc cggtagaagg
     5341 cgaggcgcgg gagaaattcc tcgctcaggc gaccgccggc aacctgatca aacggccggg
     5401 caccgcagaa gaggtcg
//
LOCUS       DBNHLM010000031         5600 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_601587, whole genome shotgun sequence.
ACCESSION   DBNHLM010000031 DBNHLM010000000
VERSION     DBNHLM010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_601587"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..385)
                     /locus_tag="AC16QF_01480"
     CDS             complement(5..385)
                     /locus_tag="AC16QF_01480"
                     /inference="COORDINATES: protein motif:HMM:NF027463.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HIR6447251.1"
                     /translation="MLKRHINMRKQDGAVSLGIVIIIGAVIGAVWLSLRLIPHYIDFE
                     TMKSVMEDLPGPQVHEMDKRAIRDSLEKRFKINNLRTFKVRDVVKIERTSKSTVIDVA
                     YEVREPLMFNIDLVLSFEDQYTYQ"
     gene            complement(409..1224)
                     /gene="lepB"
                     /locus_tag="AC16QF_01485"
     CDS             complement(409..1224)
                     /gene="lepB"
                     /locus_tag="AC16QF_01485"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR6447252.1"
                     /translation="MDIDFPLVLTWAVLISGAVWLFDLIALKPRREKAAEALTAGGIS
                     EVDAEETLKEPLIVEYARSFFPVLLLVLVLRSFLAEPYQIPSESMVPTLQVGDFILVN
                     KYAYGIRLPVLGTKIVDVGAPERGDVMVFVPPHDSRYFIKRVIGLPGDRIRYEDKALY
                     INGERANYEFIQQFRESVPFSTGYIAIREYSESFGDGRHATYRYAFNEQPKEWVVPEN
                     SYFMMGDNRGKSEDSRVWGFVPEANIVGKAVAIWVHKDPGLHWPTFSRNQWLD"
     gene            complement(1237..3030)
                     /gene="lepA"
                     /locus_tag="AC16QF_01490"
     CDS             complement(1237..3030)
                     /gene="lepA"
                     /locus_tag="AC16QF_01490"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017701262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR6447253.1"
                     /translation="MSSVASIRNFSIIAHIDHGKSTLADRLIQLCGGLADREMEEQVL
                     DSMDLERERGITIKAHSVTLSYKALDGIDYKLNFIDTPGHVDFSYEVSRSLAACEGAL
                     LVVDAAQGVEAQSVANCYTAIDQGLEVLPVLNKIDLPQAEPERVAAEIEDIIGLSAHD
                     VLCVSAKTGSGVPELLEQLISRIPPPEGDPEAPLQALIVDSWFDNYLGVVSLVRVVHG
                     RITKGDRIVAKSVGKAHIVDEVGCFTPKRSGRESLGAGEVGYVVAGIREIQGAPVGDT
                     IVNAKHQEVPQLPGFSRIKPQVYAGLFPINSEDFESFREALEKLKLNDAALFYEPESS
                     DALGFGFRCGFLGMLHMEIVQERLEREYDLDLISSAPTVVYEVVGADGQLLHIDNPSR
                     LPANYQEIREPIAEVHILVPQDYVGSVMSLCIERRGTQKKMHYSSGQVQMTWEMPLSE
                     VVMDFFDRLKSVSRGFASLDYGFDRFEASNLVRLDILINGERVDALALIAHRDQAHSR
                     GRLLAEKMKELSPRQLFDVAVQAAVGGQIVARQTVKALRKNVTAKCYGGDVTRKKKLL
                     EKQKAGKRRMKQVGRVEIPQEAFLAALKVER"
     gene            complement(3109..4497)
                     /locus_tag="AC16QF_01495"
     CDS             complement(3109..4497)
                     /locus_tag="AC16QF_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HIR6447254.1"
                     /translation="MRNMLRFGYLILLLAWVEAGFGAQLPDFVDMVAKNSPAVVNIST
                     VQSPEAKSGRPRSRNDEFEEFFRRFFGPDGSRAPYPRPRSLGSGFVISKDGFVLTNNH
                     VVEGADEILVRFNDRREMNATLVGADPRSDLALLKVEAENLPTVKLGKSKDLDVGEWV
                     LAIGSPFGFDHSATAGIVSAKGRSLPTEDNENYVPFIQTDVAINPGNSGGPLFNLDGE
                     VVGINSQIYSNSGGFMGVSFAIPIDVAMEVVEQLKESGRVARGYLGVVIQEVSRELAE
                     SFGLERPHGALVTRVLEGSPAEGGGIREGDVIVAFNGEPIDRSAELPHLVGRAKVGAE
                     AALELVREGKSMRIELVIGELADRGPGSMTSGRSGSGGSERLGLYVEPVPEAAMERLG
                     ISGGVRVVEVSGAAEEAGLRPGDIITRMNNREVADPASLREIADELPSGRSVPVLVIR
                     GQVPTFLPLRVP"
     gene            complement(4525..4944)
                     /locus_tag="AC16QF_01500"
     CDS             complement(4525..4944)
                     /locus_tag="AC16QF_01500"
                     /inference="COORDINATES: protein motif:HMM:NF016160.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HIR6447255.1"
                     /translation="MTTDLLTCRATVVASGPDGVEVGVMSPCGGCKAGCGSASRTGAR
                     MTLSSRAAVSGAPAAGTPVELAVSRSGLTKACALVFGIPIAGWLAATALVDAYWGEAV
                     ASAAGLGMLAWLLFAVRAFRPALRRWANIELIPLSRE"
     gene            complement(4948..>5600)
                     /locus_tag="AC16QF_01505"
     CDS             complement(4948..>5600)
                     /locus_tag="AC16QF_01505"
                     /inference="COORDINATES: protein motif:HMM:NF028497.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HIR6447256.1"
                     /translation="AFVRRYDRISSSYGLSLYGEDRIAGRQAVRMAVSPLDDDRFGYR
                     LWLDKETRLLLRSELIDADGNRLEIFQFNQIVFGDAVSLDALEPAGPDGSLVSHLTLA
                     RDDKTPVPESRSAWQAGWLPAGFTMAAADIRRTPSSLKSIDTMMYSDGLAAFSVFIED
                     MPPAGAASMVSRKGATVAVTHLVANAEDEHHLVTLVGELPTDTAQRIARSITFRQD"
BASE COUNT         1087 a         1829 c         1639 g         1045 t
ORIGIN      
        1 atccctactg gtaggtatac tggtcttcga agctgagcac cagatcaatg ttgaacatga
       61 ggggctcacg tacctcgtag gcgacgtcga tgacggtact cttgctggtc cgctcgatct
      121 tcacgacgtc acggacctta aaggtccgca ggttattgat cttgaaacgc ttctccaagg
      181 aatcccggat cgcccgcttg tccatttcat gaacctgcgg acctggcagg tcctccatca
      241 cggacttcat cgtctcgaag tcgatatagt gaggaatcag cctgaggctg agccagacgg
      301 cccctatcac cgcgccgatg atgatgacga ttcccaacga cacggcgcca tcctgcttgc
      361 gcatgttgat gtgacgtttg agcatcttgc acctctgcta tgctttgttt agtccagcca
      421 ttggttgcga ctgaacgtcg gccaatggag tccagggtcc ttgtgcaccc agatcgccac
      481 cgccttgccg acgatgttcg cttccggaac gaagccccac acgcggctgt cttcgctctt
      541 gccgcgattg tcccccatca tgaaatagga attctcgggc acaacccact ctttcggctg
      601 ttcgttgaac gcgtatcggt atgtagcgtg acgcccgtca ccaaaactct cggaatattc
      661 gcgaatggcg atgtaaccgg tagagaaagg aaccgattcg cgaaactgct ggatgaactc
      721 gtaattggct cgctcgccat ttatgtaaag cgctttgtct tcgtaacgga tcctgtcccc
      781 gggcaggccg atcacccgct tgatgaaata tctcgagtcg tggggcggca cgaaaaccat
      841 cacgtcaccc cgctccggag cgccaacgtc gacgattttg gtacccagca ccggcagccg
      901 gataccgtac gcgtacttgt tgaccaggat aaagtcaccg acctgcagcg tgggcaccat
      961 ggactctgac ggaatctgat acggttcggc gagaaaactg cgcagcacca gaaccagcaa
     1021 cagcaccgga aagaacgacc gggcgtactc gacgattagg ggttccttga gcgtctcttc
     1081 ggcgtcaacc tcggagattc cgccggcggt gagagcttca gccgccttct cacgtctcgg
     1141 cttgagggct atcaggtcga acagccatac cgcgcctgag atcagcaccg cccaggtcag
     1201 aaccagaggg aaatcaatgt ccattcgtct ttcgccctag cgttccactt tgagagcggc
     1261 aagaaacgct tcctggggta tttccaccct gccgacctgt ttcatgcgcc tcttaccggc
     1321 cttctgcttc tcgaggagct ttttcttgcg ggttacgtca ccaccgtagc acttggccgt
     1381 cacgtttttg cgtaacgcct tcacagtttg cctggcgacg atctgccccc ccacggccgc
     1441 ctggacggcc acatcgaaca gctgccgggg actcagttcc ttcatctttt ccgcaagcag
     1501 ccgtccgcga gaatgggcct ggtcacgatg tgcgatcagc gccagggcgt cgacccgctc
     1561 gccgttgatc aggatgtcga gacgcacgag gttgctcgcc tcgaaccgat cgaaaccgta
     1621 gtccagggag gcaaagcccc tgctgacgga tttgagcctg tcgaaaaagt ccattacgac
     1681 ttcgctcaac ggcatttccc aggtcatctg cacctgaccg gaggagtaat gcatcttctt
     1741 ctgcgttccg cgacgctcga tgcacaggct catcacgctg cccacgtaat cctgaggcac
     1801 cagtatgtgc acctcggcta tgggctcgcg tatttcctgg tagttggccg gcagccgcga
     1861 ggggttgtcg atgtgcagca gctgaccgtc tgcgccaacc acctcgtaca ccacggtagg
     1921 tgcggagctg atcaggtcga ggtcgtactc gcgctccagc cgctcctgca cgatctccat
     1981 gtggagcatc ccgagaaagc cgcagcgaaa gccgaagccc agggcgtctg acgattcggg
     2041 ctcgtagaac agggccgcgt cgttgagctt gagcttttcg agcgcctcgc gaaagctctc
     2101 gaagtcttcg gagttgatcg ggaaaagccc cgcgtaaacc tgaggcttga tccgactgaa
     2161 ccccggcagc tggggaacct cctgatgctt ggcattgacg atggtgtccc cgacgggcgc
     2221 cccctgaatc tctctgatac ctgccaccac gtagcccacc tcgccagccc cgagggattc
     2281 ccgaccggac cgtttcgggg tgaagcagcc cacctcgtcc acaatgtgtg ccttgcctac
     2341 ggacttggcg acgatccggt cgcccttggt gatgcggcca tgaaccacgc gcaccagaga
     2401 cacgacgccc agataattgt cgaaccacga atccacgatg agggcctgca gcggcgcttc
     2461 ggggtcgccc tccggcggcg ggatcctcga gatcagctgc tcgagcagtt cgggcactcc
     2521 cgagccggtc ttggcgctga cacagaggac gtcgtgcgca gataacccga tgatgtcctc
     2581 gatctccgca gcaaccctct cgggctctgc ctgggggagg tcaatcttgt tgagtaccgg
     2641 caggacctcc aggccctggt cgatcgccgt gtagcagttg gcaaccgact gcgcttccac
     2701 cccctgggcg gcatcgacga cgagcagcgc gccttcgcag gctgcaagcg atcgggaaac
     2761 ttcgtagctg aagtccacgt ggccgggcgt gtcgatgaag ttcagcttgt agtcgatgcc
     2821 atccagagcc ttgtaggaca gcgtgacgct gtgtgccttg atggtgatcc cgcgctcgcg
     2881 ttccaaatcc atggagtcga gcacctgctc ttccatttcc cggtcggcaa gaccgccgca
     2941 cagctggatc aggcgatcgg ccagggtcga cttgccgtgg tcgatgtgcg cgatgatcga
     3001 aaaattacgg atggaagcga cgctgctcaa accctgtcga accaggtgcc cgatgaaggc
     3061 gcgcgagtgt agcttatcgg gagatgccgg gccaatcggc tctcgccttc atggcacccg
     3121 cagaggcagg aatgtcggca cctgtcccct gatgacgagc accggcacgg acctccccga
     3181 gggcagctcg tcggcgatct cgcgtaggct tgccgggtct gcgacctccc gattgttcat
     3241 acgggtgatg atgtccccag gccgcaggcc ggcctcttcc gcggcaccgc tgacctccac
     3301 gacgcgaacg ccaccgctga tgccaagccg ctccatggcc gcttcaggca ccggctccac
     3361 gtaaagtcca agacgttccg atccgccgct gccgctcctg cccgaggtca tggagcccgg
     3421 accccgatcg gccagctcgc caatgaccag ctcgattctc atggatttcc cttcccggac
     3481 cagttctagc gctgcttcgg caccaacctt ggcgcgcccg acgagatgcg gcaactccgc
     3541 cgagcggtcg ataggctcgc cattgaaagc cacgatgacg tctccttcac gaatcccgcc
     3601 accctctgcg gggctgcctt cgagaacacg ggtaaccagc gcaccgtggg gacgctcgag
     3661 cccgaagctc tcggccagct cgcggctgac ctcctgaatg accacgccca ggtagccgcg
     3721 cgcaacgcgc ccggactcct tcagctgctc gaccacctcc atggcaacgt cgatgggaat
     3781 ggcgaaggag acgcccatga acccgccgga gttgctgtag atctgggagt tgatcccgac
     3841 gacctcgccg tccagattga acagcggacc accggaattg cccgggttga tggccacatc
     3901 ggtctggatg aagggcacgt agttctcgtt gtcctccgtg gggagactgc gccccttggc
     3961 gctgacgatg ccggcggttg ccgagtggtc gaagccgaac ggcgacccga tggcgagcac
     4021 ccactcaccc acgtccagat ccttcgactt gccgagctta accgtcggca ggttttccgc
     4081 ttcgaccttg agcagggcaa ggtccgaacg agggtctgcc cccacgaggg tggcgttcat
     4141 ttctcgccgg tcattgaagc gcacgagaat ctcgtcggcg ccttccacga cgtggttgtt
     4201 cgtcagcacg aagccatcct tggagatgac gaagccggaa cccaaagaac ggggcctcgg
     4261 gtagggcgcc ctgctgccat ccgggccgaa gaatcgccgg aagaactctt cgaactcatc
     4321 gtttcgcgag cggggacgcc cggatttggc ctcagggctc tggacggtgc ttatgttcac
     4381 aacggccggc gagttcttgg cgaccatgtc cacgaaatca ggcagctgcg ccccaaagcc
     4441 cgcctctacc caggcaagga gcagaatcag gtaaccaaat ctaagcatat ttctcataca
     4501 accatcggtc gcctcgcggc gccattactc gcgtgaaagc gggattaact caatattggc
     4561 ccagcgccgc aacgcaggac gaaacgcccg aaccgcaaac agcaaccagg cgagcatgcc
     4621 aagccccgcc gccgaggcca cggcttcgcc ccaataagcg tcgaccagcg ccgtggcggc
     4681 cagccagccc gcgatcggga taccgaaaac cagggcacag gccttcgtca gaccgctgcg
     4741 ggacacggca agctcgaccg gcgtgccggc cgccggggca ccgctcacag cggcccggga
     4801 agacagggtc atcctcgcgc cggtcctgga cgcgctcccg cagccggctt tacagccacc
     4861 gcagggcgac atcaccccca cctcgacccc atccgggccg ctcgccacca cggtggcacg
     4921 gcaggtgagc aggtccgtcg tcatcaatca gtcctgccgg aacgtgatgg accgggcaat
     4981 tctctgcgcg gtgtccgtgg gcaactcacc caccagcgtc accaggtgat gttcgtcttc
     5041 cgcattcgcc accagatgcg tgaccgccac cgtggcgccc ttacgggaaa ccatgctggc
     5101 ggcgcccgct ggcggcatgt cctcgatgaa tacggagaat gccgccaacc cgtccgaata
     5161 catcatggtg tcgatagatt tcagagaact gggcgtgcga cggatgtccg ccgcggccat
     5221 ggtgaacccg gctggcagcc accctgcctg ccaggcactg cgggactccg gcaccggcgt
     5281 cttgtcgtcc cgcgcaaggg tcaggtggga aaccagggag ccgtccggtc cagcaggctc
     5341 gagggcgtcc aggctgaccg cgtcgccgaa gacgatctgg ttgaactgaa agatctccag
     5401 gcggttgccg tcggcgtcga tcagctcgga acgcagcagc agccgggtct ccttgtccag
     5461 ccacaggcga tagccgaagc gatcgtcgtc tagcggggaa acggccatgc gaaccgcctg
     5521 acgccccgcg atgcggtcct cgccgtagag cgagagccca taggaactcg aaatgcggtc
     5581 gtagcgccgg acgaaggcgc
//
LOCUS       DBNHLM010000032         4101 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1200761, whole genome shotgun sequence.
ACCESSION   DBNHLM010000032 DBNHLM010000000
VERSION     DBNHLM010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1200761"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1399..1845)
                     /locus_tag="AC16QF_01510"
     CDS             complement(1399..1845)
                     /locus_tag="AC16QF_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6447257.1"
                     /translation="MPYKHVLVAVDLSEESTQVLDKAREAADGNGAKLSLLSVVKPLT
                     QVYGGLDMAPIASGAVSFEEEALQRAEAQLKDLGSRYGVADDDVHVLLGAPAHEIREA
                     AKAYAADLIIMGTHGRHGLGLLLGSTANAVLHGLECDVLVVRVHFE"
     gene            2026..2355
                     /locus_tag="AC16QF_01515"
     CDS             2026..2355
                     /locus_tag="AC16QF_01515"
                     /inference="COORDINATES: protein motif:HMM:NF045308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR6447258.1"
                     /translation="MNTQEAKSRLEARRAELTDRQERVARHTRHRDEPLSQDFAEQAV
                     ELENGETLVALDREISQELVQIDHALKRIDADTYLICEGCGEPVGEERLKALPYASRC
                     IDCASER"
     gene            2374..2808
                     /locus_tag="AC16QF_01520"
     CDS             2374..2808
                     /locus_tag="AC16QF_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6447259.1"
                     /translation="MKEPQLKTAMTPFPYAVELSAPIAQARKLMLEHHVHHLPVTEDH
                     ELKGIITDRDIKLLLGPELGSPDPKELTVEDAYIDDCYAVDMGTPLRQVLTTMAARHI
                     GAAVVTAHGRLAGIFTATDACRVFAELLNAQFGSGSEPPDAA"
     gene            complement(2814..>4101)
                     /locus_tag="AC16QF_01525"
     CDS             complement(2814..>4101)
                     /locus_tag="AC16QF_01525"
                     /inference="COORDINATES: protein motif:HMM:NF025052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside
                     phosphotransferase/ATP-binding protein"
                     /protein_id="HIR6447260.1"
                     /translation="GRIRVGGTGEVVEHAVKMRQFPQSSALDRLLVAGVLEPGGLSRF
                     GLELATLHAGLPRLEAPDDELDRRILGPVLDNFATLRATRAGGDRSDILQRLESASKD
                     AFDGKRTTFSRRLAKGFVRECHGDLHLRNLVMIDGRVRAFDCLEFNPDLRWIDIASDA
                     AFLIMDCAVRDRVDLAYAFQDGYLAGCGDYVGATLSPFYQAYRSVVRAKVAALEIEGS
                     PKDGKLGAARERLDRHLNWAWERLHGPRGRMVLMCGLSGSGKSYVAERLVPRLPAVRI
                     RSDVLRKVAAGLEPLQRSGSSVDAGLYTPSRSQSVYAEMQAIALALAAAGETVIVDAT
                     FLERAARKAFRGAAAAAGVPCVVLHCQASEPTMRSRVAAREREGRDASEAGIAVLEAQ
                     QSRFEPPTADEETVVVDTETTAVMAQIEGALRAAFA"
BASE COUNT          800 a         1411 c         1161 g          729 t
ORIGIN      
        1 gtcgggtgta cgaaccccaa cgtgcccttc ttgcgcccga cggccgatct cgccgcatcg
       61 acaattacca cttctctcat ggtctgcttt cctcgcttaa tcaatcaaca atcaagtttc
      121 ccttcaccgg aacggcatct tcaacccgcc ccgcaagcga cgtcggcaac atcgcgccca
      181 acgccaacga tccgccagcg ccgctccgga taatgacgtc aaaacgggga catggacccg
      241 acccgcctcg gccaggagat cgtgcaggtt atctggaagt gtctcccttt ggaaccaatc
      301 ctgagcctct ggctccagcc ccgacagatc ctccaccgca ccgacccacg cgcacacgtc
      361 gagcaatgct cgactaccga actagccgcc gatctgcccc tcacgccaaa cgcgctgcag
      421 atacgagcga ttgccgattc gtgcctgaga accttccggt aacttgttga ttctaggtgg
      481 gcgaactgtt agatttccca acgatttcgc cctgccaggt cgatgaggac gctgcagatt
      541 cgtgggggga ggggaaatcg gacaatcgga tcccggacaa caacaaaaat ctagggatat
      601 ttaggccgcc tccgggcggc tttgtttttt ccgcctctta tcgctcaccg caccttcgct
      661 tttatccatg gaccgattga ccatcggcgg cataacgcgg taagcgaaaa aagaaaaccc
      721 cgccgaagcg gggttccctg aacgttgccc gaccgaagcc tggcaacccg gtgagcgtga
      781 tgttcgcgcc ttgcggccgg ttcccacctc tcatccggag gcccctttac tctcctgccg
      841 ccatcgacgg cactcgaggt ccacggccac gactcgagag tcgttattta acgacgcctt
      901 gctacgtcgc tccgatccgt tgcgatcact ccggatcgaa gggcggaagc aagcccctca
      961 actgccccac gtttccggcg taagcttccc ctaagggacg ccctccttcc agcgagccag
     1021 ccttcgcacg agcaagccat cggccaacgg ctctcccatg ctcgggactc tcccttgagc
     1081 tgccggcgcc tccggcaacc ccgaccctgg cgggggtctt ccggtcttgc gtttcgcggc
     1141 cccctttcag gggctttgac cgcagaccgt ggacacagtg ttacccaact ggtcggagct
     1201 gaccaatgaa acatccgcaa ggttgtgtgg atatgttgtg gatcagaccg tcgcaatcct
     1261 gtggaaaagt tcacgcaagc ttgcccgcgt ttgagctcgc gctgccaagc aactgaaagc
     1321 tcgacttttt ccgcacatgc atctcgttca atacgcaagg cctcgagcgc aacgtcaatt
     1381 ggcgcggtcg cgtccgcttc actcgaagtg cacgcgcacg accagcacgt cgcattccag
     1441 cccgtgcaga acggcgtttg ccgtggagcc gagcaggagc cccaggccgt gccggccatg
     1501 agtgcccatg atgatgagat cggcggcgta agccttggcc gcctcacgga tctcgtgcgc
     1561 gggcgcgccg agcagcacat ggacgtcatc gtccgccacg ccgtaacggc tgcccaggtc
     1621 cttcaactgg gcttccgctc gctgcagcgc ttcctcctcg aaggaaaccg cgccgctggc
     1681 aatgggggcc atgtccagac caccgtagac ttgagtcagc ggctttacca cgctcaagag
     1741 agacagttta gccccgttgc cgtccgcggc ttcccgagcc ttgtcgagta cctgcgtcga
     1801 ctcttcggac agatctaccg ccaccagcac gtgtttgtac ggcatcgggc cacctcccgt
     1861 gatcatttct ccttgagatt agcccaccat cgcagcgaga gccatccttg tcggtaccgg
     1921 gaacttcggc gatcctctat tcggaaactg cgtaaggacg gcgggcagcg agaggcggta
     1981 tgatctaccc gaacacgttc aagccggacg aactcggagc atacagtgaa cacacaagaa
     2041 gccaaatcga ggctggaggc gcgccgggcg gaactcacgg accggcagga gcgcgttgcc
     2101 cgccacacca gacaccggga cgagccgctg tcgcaggatt tcgccgagca ggcggtggag
     2161 ctggaaaacg gagagaccct cgtcgctctg gacagggaga tcagccagga gctcgttcag
     2221 atcgatcacg ccctgaaacg aattgatgca gacacctatc tgatttgcga gggatgcggg
     2281 gagcccgtcg gtgaggagcg actgaaggct ctgccctatg ccagccgctg catcgactgc
     2341 gccagcgagc gctgaaccgg atccggcagc ctggtgaagg aaccgcagct caaaacggcc
     2401 atgacgcctt tcccctacgc cgtcgagctc tcggctccga tcgcccaggc ccgaaagctc
     2461 atgctcgaac accatgtcca tcatcttccg gtcacggaag atcacgagct caaaggcatc
     2521 attaccgacc gggacatcaa gcttctgctc ggccccgaac tcggctcccc ggatcccaag
     2581 gagcttaccg tagaggatgc ctacatcgac gactgctacg ccgtcgacat ggggacacct
     2641 ctgcggcagg tgctgactac catggctgcg cggcacatcg gcgcggccgt ggtcaccgct
     2701 cacgggcgac ttgccggaat cttcaccgcc accgatgcgt gccgcgtttt cgcggagctt
     2761 ctcaacgccc agttcgggtc cggcagcgaa ccaccggacg ctgcctgata ggctcaggca
     2821 aacgccgccc gaagggcccc ctcgatttgc gccatcacgg ctgtggtttc cgtgtcgacc
     2881 acgaccgttt cctcatccgc tgtgggcggc tcaaatcgcg actgctgcgc ctctagcacc
     2941 gcgatgccgg cctcggaggc atccctgccc tcccgctccc gggctgccac ccgtgacctc
     3001 atcgtgggtt cggatgcctg gcaatgcagg acaacgcagg gcacgcccgc cgccgccgcg
     3061 gcgccgcgga aagcctttct tgccgcgcgc tcgagaaaag tggcgtccac gatgaccgtt
     3121 tcgcctgcag cagctaaagc cagcgcgatt gcctgcatct cggcgtaaac cgattggctc
     3181 cgtgaaggcg tgtacagccc cgcatccacc gatgatccgg atcgttgcag cggttccagg
     3241 ccggctgcca ccttgcggag cacgtcagat cggatacgca cagcgggcaa tcgcggcacg
     3301 agccgctccg ctacatacga ttttccagag ccggatagac cgcacatcaa aaccatcctg
     3361 ccccggggcc catgcagacg ctcccaggcc cagttcaggt gccggtcgag acgttcccgg
     3421 gcagcgccca gcttgccgtc cttcggcgac ccttcgatct ccagggcagc caccttggct
     3481 cggacgacgg atcggtaggc ctgataaaaa ggggacaacg tcgccccgac gtaatctccg
     3541 cagccggcca ggtagccgtc ctgaaaagcg taggccaggt caacgcgatc tcgcaccgca
     3601 cagtccatga tcaggaaagc ggcgtcgctt gcgatgtcga tccagcgcag gtcggggttg
     3661 aactcgagac agtcgaacgc cctcacccgc ccgtcgatca tgacgagatt acgcaggtgc
     3721 agatcgccgt gacactctcg cacgaagccc ttggccaggc ggcgcgagaa cgttgttcgc
     3781 ttgccgtcaa aagcgtcctt cgaggccgac tcgagccttt ggaggatgtc cgagcgatcc
     3841 ccgccggccc gggttgccct gagcgtcgcg aagttgtcca gcaccggccc caggatccgc
     3901 cggtccagct cgtcgtccgg ggcctccaga cgtggcagcc cagcatgcag agtggccagc
     3961 tccagtccga atcgactcaa cccaccgggc tcgagaacgc ctgcgaccag aagccgatcc
     4021 agcgcgctgc tctgaggaaa ctgccgcatc ttcactgcat gctcaacgac ctcgccagtc
     4081 ccgccaaccc ggatccggcc c
//
LOCUS       DBNHLM010000033         9918 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1200932, whole genome shotgun sequence.
ACCESSION   DBNHLM010000033 DBNHLM010000000
VERSION     DBNHLM010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9918
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1200932"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="AC16QF_01530"
     CDS             complement(<1..773)
                     /locus_tag="AC16QF_01530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6447261.1"
                     /translation="MQARPPKPFKARDLTADRATAMRFTAVLGLQMGQIFPGSFIALF
                     LPVIYREQGLSLEMYWVFTLPAIPTWIRPLWAPFVDRTGSRIIGMRKTWFIPCTVFGA
                     LAYLSLTQFAPTLENLAVIITILVIKTTFMTTQDIAIDGYMVENIRDEERPVGAAVLD
                     IGRNVARFIAWAGIAWVYTEHGWSAAVTVASALLILFSAPGIIRREPPRPSQFDHVRP
                     SLKNLLRRRDSYYVYPLCFGIALMGALVQSLYPTFLSDLG"
     gene            980..1918
                     /locus_tag="AC16QF_01535"
     CDS             980..1918
                     /locus_tag="AC16QF_01535"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR6447262.1"
                     /translation="MQFKNRVTEMLGVEIPVVQAPMGWIARSQLASAVSNAGAMGIIE
                     TSSGELDAVREEIRRMRELTDKPFGVNIAQAFVRDPNIVQFVIDQGVKFVTTSAGNPE
                     RFTDELKSAGLTVFHVVPSLSAALKAVACGVDGLVVEGGEGGGFKNPREVSTMVLLPL
                     VRSKVDVPIIAAGGFVDGASMAAAFALGAEGVQLGTRMVSALESPVHQNWKDAIVNAG
                     ETDTVFLNRKHSPALRALRTEKTTRLENEPDTNAMSEFGSALDLYFGGDMEASIALSG
                     QVCGRIDAVRPVAEIIAELKDEFFGTIARLDEQYLS"
     gene            complement(1921..3249)
                     /locus_tag="AC16QF_01540"
     CDS             complement(1921..3249)
                     /locus_tag="AC16QF_01540"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019580362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR6447263.1"
                     /translation="MTNSYLISNARIVNEGTITEGDLLVENGRISGIGKPAPAGTDVF
                     DANGAWLLPGMIDDQVHFREPGFEHKGTIATESRAAIAGGITSYMEMPNCNPLTVTHE
                     ALADKHVRAKDHSAANFAFYFGATNDNLEAIKTVDPALTCGIKVFMGASTGNMLVDDA
                     DTLEGIFAGTPLIVATHCEDTPTILANEERAREAFGDAIPFSEHPRIRSEEACWLSSS
                     MAVGLARRHGTRLHVLHLTTAREMALFEPGDLMGKQITAEACVHHLFFNESWYPDKGA
                     DIKCNPAIKGAADQEALLRAVAEDRIDVIATDHAPHTREEKDREYLRAPAGLPLVQHA
                     LSSLVEQVHRGVFDIETVVRKTAHAPALLFGVKERGFIREGYHADLTVIDGDALVDGG
                     EIHSKCGWSPFSGIDLHGRVRATWVNGVLRYRDGRILDGPSGIALEFERP"
     gene            3384..4838
                     /locus_tag="AC16QF_01545"
     CDS             3384..4838
                     /locus_tag="AC16QF_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014590532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR6447264.1"
                     /translation="MNHASALRQLRAALPEGAVLTDRHQLKPYETDGLTALRQMPWAV
                     ALPQTEAEVREVLRICAEHEVPLVPRGAGTGLSGGARPVSDGLVLGVSRLDRILEIDV
                     ENGVARVEPGVRNLAISEAASAYGLYYAPDPSSQIACSIGGNVAENSGGVHCLKYGLT
                     VHNVLGLKVLTVEGDELQIGGKRLDSPGYDLLALMSGSEGMLAVITEITVRLLPIPPE
                     KRVLLGAFRTVAAAAEAVNQVISEGLVPAGLEMMDRLAIEAAEDFVHAGYPREAEAIL
                     LCEVDGVTEEVDNDVDLVRRVFETLGAFDVRIATDPAEQANLWKGRKAAFPAVGRISP
                     DYYCMDGTIPRYELARVLAEIASLATEFGLRVANVFHAGDGNLHPLILYDAGREGELA
                     KAEALGGKILELCVEVGGTVTGEHGVGVEKLDQMCAQFSPEELAQFFGVKACFDPGQT
                     LNPGKAIPTLARCSEVGGMHVHGGRLPYGNLERF"
     gene            4839..5897
                     /gene="glcE"
                     /locus_tag="AC16QF_01550"
     CDS             4839..5897
                     /gene="glcE"
                     /locus_tag="AC16QF_01550"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: protein motif:HMM:NF008439.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HIR6447265.1"
                     /translation="MAGLDRDDSERLVEAVRDAYARNQPLAIVGSGSKSFLMPADDGA
                     RLLSVAEHAGVLEYRPDELVVTARAGTPLKDLQQLLSQHRQMLPFEPPLFGGGGTLGG
                     ALAAGLSGPGRPWRGSARDALLGVELINGRGERLRFGGKVLKNVAGYDLSRLQVGAFG
                     TLGVLLAASVRVVPAPQAERTCLLDMPAAPALQTVRALCARPVPLTATCFAEGSLRLR
                     FSGSEAAVLSASRDAGGELVDGDRYWQDLRDHRLPVMRPDVPLWRVSLPPGADPALQD
                     GVVEWGGALRWLVAVDPGPGELAAGLGGWAARFGAGYARAVCDALPPPQMKYQRRLKR
                     NFDPENLFNPGLVGTDAD"
     gene            5887..7086
                     /gene="glcF"
                     /locus_tag="AC16QF_01555"
     CDS             5887..7086
                     /gene="glcF"
                     /locus_tag="AC16QF_01555"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HIR6447266.1"
                     /translation="MQIELHDDFAETADGQRAQDILRACVHCGFCNATCPTYQVTGDE
                     LDGPRGRIYLIKEMLEEGSANRVAERHLDRCLTCRACETTCPSGVAYGELLEVGRELV
                     ERDRRRDPIASFLRAWLLAVVPDPGRFRRWARLGGWFRWLLPGRLGAALPKVGPEQPV
                     SRTDASRSVLVLQGCVQRTATPGVNAALIDLLNARDYDVVYARNEGCCGSLNLHLAEK
                     ERALADARRLLDVVAEDLEEVEAVISTASGCGVTLKDYPRLLADDPAYADRARALAGK
                     LVDAAEFFASRELELERAFDGLRVAWQAPCTLQHGQKITGMVEKVLGAAGYELVSVVD
                     PHLCCGSAGTYSILQPEMAGELRRRKLATLCAQTPDVIATANVGCQTHLAAESPVPVR
                     HWLELLR"
     gene            7168..7569
                     /locus_tag="AC16QF_01560"
     CDS             7168..7569
                     /locus_tag="AC16QF_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6447267.1"
                     /translation="MKITRINHAAVNVTGKVEEAHDFYVKLLGLPQVPIQLPGAPPVM
                     ESALGFWVEQDGVQLHVIGREPFGGTPDPTQNHVSWFVEDLDVAVGELEQNGIEMRVM
                     GEGSGRIIWVLDPGGNIVEFQQDPDLAPPPA"
     gene            7591..8742
                     /locus_tag="AC16QF_01565"
     CDS             7591..8742
                     /locus_tag="AC16QF_01565"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6447268.1"
                     /translation="MRFESRRESFAELESARGLVPGIRTSQCEIDKQQALPADLAARL
                     AADGMFRLLLPVCIGGSGLSLPRFVRVVEVIAEADGSTGWCVSQAAVFASVADLLHAE
                     TAAEIWGTNRDAAVATGIPFGAEARAVDGGYLLDGRWRFASGCTHADWFAPLASVVGD
                     VGSEPSRTFLVPREEVRLGEGWNVRGMQGTGSREYRLHALHVPSRRSVADAYTSHPGA
                     STGLHVMLLFACGFAAVGLGVARRAVDEFIALAAGKTPVFANRKLAEDDLVQSGLGEA
                     EALLGATRAFLLDAAEECEHRHATTGAVDFELRSRMRLAATHAMRRSADVVDRVYAMA
                     GTDCIFDDHPLQRCFQDIHALTQQVQGRASHYRSVGRVLLGRQKETFAF"
     gene            complement(8759..9220)
                     /locus_tag="AC16QF_01570"
     CDS             complement(8759..9220)
                     /locus_tag="AC16QF_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6447269.1"
                     /translation="MTAGCATSSVLPPVVDTNGIEDAEPAPQQEVPIPPASTPEARQP
                     DTAGATLALLEQSERAASAGDADAAVAYVERAIRLNPRSADLWLQLARLQLANEDPEA
                     AVQFANKAISLAGDRLDWVRDAWLLIADARAAQGDGDGAREIRERWRGYRG"
     gene            complement(9244..>9918)
                     /locus_tag="AC16QF_01575"
     CDS             complement(9244..>9918)
                     /locus_tag="AC16QF_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR6447270.1"
                     /translation="AARFETLIGRPPANTYPSLLLGAEAMTPVEVSALYGIFASGGFY
                     QPPKAVISVLDESRSPTLRRPLKLEQRIDTKTARALDRALQVVMARGTGRSSRFARVG
                     VAGKTGTSDDYRDSWFAGYDDAALTVVWVGRDDNAPTGMTGASGALKVWDRIMADRDV
                     IAIPPDPDSRKVDVEFATGLVANPLCADVVTIPVPEGAALQEKPGCGIKPPSLGERIR
                     NWLRND"
BASE COUNT         1540 a         3120 c         3461 g         1797 t
ORIGIN      
        1 cccagatcgg aaagaaacgt tgggtacagc gactgcacca gcgcgcccat cagagcgatt
       61 ccaaagcaga gagggtagac gtagtagctg tcgcgacggc gaagcaggtt cttgaggctc
      121 ggccggacat ggtcgaactg ggacggcctc ggcggttccc tgcgaatgat tcctggtgcg
      181 gagaacagga tcagcagagc cgaggccacg gtgaccgcgg ccgaccagcc atgctccgtg
      241 tagacccagg cgatgccggc ccacgcgatg aacctggcca cgttgcggcc gatgtccagc
      301 acggcggcgc ccaccggccg ctcctcgtcc cggatgttct cgaccatgta gccgtctatg
      361 gcgatgtcct gggtggtcat gaacgtggtc ttgatcacca gaatcgtgat gatcacggcg
      421 agattttcga gggtgggcgc gaactgggtc aggctgagat acgcaagagc gccgaagacc
      481 gtgcagggaa tgaaccaggt ttttcgcatg ccgatgatgc ggctgcccgt ccggtcgacg
      541 aacggcgccc agagcggccg tatccaggtc gggatggcgg ggagcgtgaa gacccagtac
      601 atctccagcg acagcccctg ctcgcggtag atcaccggca gaaacagggc gatgaacgag
      661 cccggaaaga tctgacccat ctgaaggccg agcaccgcgg tgaagcgcat ggccgtcgcc
      721 cgatcggcgg tcaggtcgcg cgctttgaac ggcttgggtg ggcgggcctg catccggcca
      781 ctttagcggc ggcgcccgga tggcacacgg gttgtttctg cgatcgacgc tccagtagaa
      841 tccgcggccc tttccggcca cccgcaccct gctgggttcc tctatttcgg gctggccgtc
      901 ttcctctgac gattcggcct gcgccactac acgtccaccg gcagctgagg taggctcccc
      961 gatttccccg gggggcgtca tgcagttcaa gaaccgagta accgagatgc tgggggtcga
     1021 gatacccgtc gtgcaggctc cgatgggatg gattgcccgc tcgcagctcg cctccgccgt
     1081 gtcgaacgcg ggcgccatgg gcatcattga aacgtcctcc ggggagctcg acgccgttcg
     1141 ggaggaaatc cgcaggatgc gcgagctcac cgacaagccg ttcggtgtca acatcgccca
     1201 ggcgttcgta cgcgacccca acatcgttca gttcgtcatc gaccagggcg tcaagttcgt
     1261 gacgacgtct gccggcaatc cggagcggtt cacggatgag ctcaagagcg ccgggctcac
     1321 cgtctttcac gtggttccca gcctcagcgc tgcgttgaag gccgtggcct gcggcgtgga
     1381 tggactggtg gtggaaggcg gcgagggcgg cggcttcaag aacccacgcg aggtctccac
     1441 catggttctg ctgcccctgg ttcgctcgaa ggtggatgtt cccatcatcg ccgcgggcgg
     1501 gttcgtggac ggcgcgtcca tggcggcagc gttcgcgctc ggcgcggagg gcgttcagct
     1561 cggcacgcgg atggtgtccg cgctggagtc acccgtgcat cagaactgga aagacgccat
     1621 cgtgaatgcc ggcgaaacgg acacggtgtt tctcaaccgc aagcactcgc ccgcgctgag
     1681 agcgctgcgc acggagaaga cgacgcggct ggagaacgaa ccggacacca acgccatgag
     1741 cgagttcggc tccgcgctgg atctctattt cggtggcgac atggaagcga gcatcgctct
     1801 gtcgggtcag gtttgcggcc gcatcgacgc cgttcgcccg gtggcggaga tcatcgccga
     1861 gctgaaagac gagttcttcg ggaccatcgc gcgcctggac gagcagtatc tgagctgagt
     1921 tcagggccgc tcgaactcca acgcgatgcc gctcgggccg tcgaggatcc ggccgtcgcg
     1981 gtaacggagc acgccgttca cccaggttgc ccggaccctg ccgtgcagat cgatgccgct
     2041 gaacgggctc cagccgcact tggagtggat ctccccgcca tccaccaggg catcgccgtc
     2101 gatcacggtc aggtcggcgt ggtatccctc gcggatgaag cctctttcct tgacgccgaa
     2161 gagcagcgcc ggtgcgtggg ccgttttgcg gaccacggtc tcgatgtcga acacgccgcg
     2221 gtggacctgc tccaccagcg atgaaagggc gtgctgcacc agcggcagcc cggccggggc
     2281 gcgcaggtac tcgcgatcct tctcctcccg ggtgtggggg gcgtgatcgg tggcgatcac
     2341 gtcgatgcgg tcttccgcca ccgcgcgcag gagggcttcc tggtcagcgg cgcccttgat
     2401 ggcagggttg cacttgatgt cggcaccctt gtcggggtac cagctctcgt tgaaaaagag
     2461 gtggtgcacg caggcttcgg ccgtgatctg cttgcccatc aggtctcccg gctcgaaaag
     2521 cgccatctcg cgcgccgtgg tcaggtgcag cacgtggagc cgggtgccgt gtctgcgggc
     2581 gagaccgacg gccatggacg atgacagcca gcaggcttcc tcggacctta ttcgagggtg
     2641 ctccgagaac gggatggcgt cgccgaacgc ttccctggcg cgttcctcgt tggccaggat
     2701 ggtgggcgtg tcctcgcagt gggtggccac gatcagcggc gtgccggcga aaatgccctc
     2761 taaggtgtcg gcgtcgtcca ccagcatgtt gccggtgctc gcccccatga agaccttgat
     2821 tccgcaggtg agggcgggat ccacggtctt gatggcctcc aggttgtcgt tggtggcgcc
     2881 aaagtagaag gcgaaattcg cggcggaatg gtcctttgcg cgaacgtgct tgtcggccag
     2941 cgcctcatga gtaaccgtca gcgggttgca gttcggcatc tccatgtagc tcgttatgcc
     3001 tccggcaatg gccgcgcggg attccgtggc gatggttccc ttgtgctcga aaccgggctc
     3061 tcgaaagtgc acctggtcgt cgatcatccc gggcaggagc caggcgccgt tggcgtcgaa
     3121 aacgtcggtg cctgccggtg ccggtttgcc gatgccggag attcggccgt tctcgacgag
     3181 gagatcacct tccgtgatgg tcccctcatt gacgatccgg gcgttgctga tgagatagct
     3241 gttggtcatg aagtctggtc cgttcgccgc gcggtgcttc ccgtgggcgg tttctgcgtt
     3301 ggggttactt tagccgattt ggcgggcagg atgcgctcgc cgacgtgaaa gacgccgtct
     3361 tccatggcgt atactcctcc accatgaacc atgccagcgc tctccgacag ctgcgtgccg
     3421 ccctgcccga gggagccgtt ctcaccgatc ggcaccagct caagccctac gaaacggacg
     3481 ggctcaccgc gctcaggcag atgccctggg ccgtggcgct tccacagacc gaggcagagg
     3541 tgcgggaggt gctgcgaatc tgcgccgagc acgaggtgcc tttggttccg cggggggccg
     3601 ggaccgggct ctccggcggg gcgaggcccg tcagcgacgg cctggtgctg ggggtttccc
     3661 ggttggatcg aattctcgaa atcgacgtgg aaaacggtgt ggcccgggtc gagccaggcg
     3721 tccgcaacct ggccatcagc gaggcagcat ccgcttacgg gctttactac gcgcccgacc
     3781 cttcctccca gatcgcctgc agcatcggcg gcaacgtggc ggaaaactcc ggcggcgttc
     3841 attgcctgaa gtacggcctc accgttcaca acgttttggg gctgaaggtg ctcaccgtcg
     3901 aaggcgacga gctgcagatc ggcggtaagc gcctagactc tcccggttac gatctgctcg
     3961 cgctgatgtc gggctcggaa ggcatgctcg cggtgatcac cgagattacc gtgcggctcc
     4021 tgccgatacc gccggaaaag cgggtgctgc tcggggcgtt ccgcacggtc gcagcggcgg
     4081 cggaggccgt gaatcaggtt atctcagagg gtctggtgcc ggcaggattg gagatgatgg
     4141 accggctcgc catcgaggcg gccgaggact tcgtgcacgc cggttacccg cgggaggcgg
     4201 aagccattct gctgtgcgag gtcgacggcg tgaccgagga ggtggacaac gacgtcgacc
     4261 tggttcgcag ggtgttcgaa acgcttggtg ctttcgacgt gcgcatcgcc actgatccgg
     4321 ccgagcaggc caatctgtgg aagggccgca aagccgcctt tcccgcggtg gggcgtatct
     4381 ctccggacta ctactgcatg gacggaacca taccccgcta tgagctcgcg cgcgtgctgg
     4441 ctgagatcgc ttcgctggca acggagttcg ggcttcgagt cgccaacgtc ttccatgccg
     4501 gcgacggcaa tctgcaccca ttgattcttt acgacgcggg gcgcgaaggt gagctcgcga
     4561 aagccgaggc ccttggcggc aagatcctcg agctttgcgt cgaggtgggc ggcacggtga
     4621 ccggagagca cggtgttggc gtcgagaagc tcgatcagat gtgcgcgcag ttctcgcccg
     4681 aggaactcgc ccagttcttt ggggtgaagg cctgcttcga tcccggccag accctcaatc
     4741 cgggcaaggc gatcccgact ttggcgcgct gctcagaagt cgggggcatg cacgtccacg
     4801 gcggtcgtct cccgtacggc aatctggagc ggttctagtt ggccggcctc gaccgggatg
     4861 acagcgaacg tctggtggag gcggtgcgcg acgcgtatgc caggaaccag ccgctcgcca
     4921 tcgtcggttc cggcagcaag tcgtttctga tgcccgcgga cgatggcgcc cggctgctct
     4981 ccgttgcgga gcatgcgggc gtgctggagt accggccgga cgagctggtc gtcaccgccc
     5041 gggcaggcac cccgctcaag gatcttcagc agctgttgtc gcagcaccgg cagatgctgc
     5101 cgttcgaacc tccgctgttt ggtgggggcg gcacgctggg cggtgctctg gccgccggtc
     5161 tttccggccc cggacggccg tggcgggggt ccgcccggga cgcgctgctc ggcgtcgagc
     5221 tgatcaacgg gcgcggcgaa aggctgcggt ttggtggaaa ggttctcaag aacgtcgctg
     5281 gatacgacct ttcgcgcctg caggttggcg ccttcggcac gctcggcgtg ctgctcgccg
     5341 cgagcgtgcg ggtggtgccg gcgccccaag cggagcgaac gtgccttctg gacatgccgg
     5401 ccgcgccagc gttgcaaacg gtgcgcgcgc tgtgcgcccg cccggtaccg ctgaccgcca
     5461 cctgtttcgc cgagggcagc ctgcggctgc gcttctccgg gtccgaggcc gccgtgctga
     5521 gcgcttctcg cgatgccggc ggagagctgg tcgacggaga ccgctactgg caggatctgc
     5581 gggaccaccg actgcccgtc atgcgccccg acgtcccctt gtggcgcgtg agccttccgc
     5641 ccggagcaga cccggcgctg caggacggcg tggtggagtg gggcggcgcg ctgcgctggc
     5701 tcgtcgccgt cgatccgggt ccgggggagc tggccgccgg ccttggaggc tgggcggcga
     5761 gattcggtgc cggatacgcc cgtgccgtct gtgacgccct gcccccgccg cagatgaaat
     5821 atcagcggcg gctgaagcgg aacttcgatc cggaaaacct gttcaacccg ggattggtgg
     5881 gcacagatgc agattgagct gcacgacgac ttcgcggaaa cggccgacgg gcagagggcg
     5941 caggacatcc tgcgcgcctg cgttcactgt ggtttctgca acgcgacgtg cccgacgtat
     6001 caggtcacgg gggacgagct ggacggtccg cgcggccgta tttacctgat caaggagatg
     6061 ctggaggagg gctcggccaa ccgcgtcgcc gagcgtcatc tggatcgctg tctgacctgc
     6121 agagcctgcg agaccacgtg tcccagcggc gtcgcttacg gcgagctgct ggaggtgggt
     6181 cgcgagctcg tggagcgtga ccgccgccgc gatcccatcg cttcgttcct gcgcgcctgg
     6241 ctgcttgccg tggtcccaga ccccgggcgc tttcgccgct gggcgcgtct gggcggctgg
     6301 ttccgctggt tgcttcccgg acgtctgggt gccgcgctac cgaaggtggg cccggaacag
     6361 cccgtttcca ggacagacgc gtcgcgcagc gtgctcgtcc tgcagggctg cgttcagcgc
     6421 acggccacgc cgggcgtcaa cgcggcattg atcgatctgc tcaatgccag ggactacgac
     6481 gtcgtctacg cccgcaacga aggctgctgc ggcagcctga atctgcatct tgcggaaaag
     6541 gaacgggccc tggcggacgc ccgccgcctg ctcgatgtcg ttgcagagga ccttgaagag
     6601 gtggaagcgg tcatatccac ggccagcggc tgcggcgtga cgctcaaaga ctacccccgg
     6661 ttgctggcgg acgaccccgc ttacgctgat cgcgcgcggg ctctggcggg aaagctcgtc
     6721 gacgccgccg agttctttgc ctcccgggag ctggagctcg aacgtgcctt cgatggcttg
     6781 cgagtggcat ggcaggcgcc gtgcaccctg cagcacgggc agaagatcac ggggatggtg
     6841 gagaaggtcc tcggcgcggc cggctacgaa ctcgtctccg ttgtggaccc ccacttgtgc
     6901 tgcggctcgg ccgggaccta ctccattctg cagccggaga tggccggcga gcttcgccgg
     6961 cgcaagctcg ccacgctgtg cgcgcagacg cccgacgtga tcgcgacggc caacgtgggt
     7021 tgccagaccc acctggccgc ggagagcccc gtgcccgttc gccactggct ggagctcctg
     7081 cgctagggcc cgtccatggg agcggggatt gcggtggtag gcgcagtcca cttaaatgaa
     7141 acgcgaggtg gcataaaggg gagattcatg aagattacgc gtatcaacca tgcggcggtg
     7201 aacgtcaccg gcaaggtgga agaggctcac gatttttacg tcaagctgct cgggttgccc
     7261 caggtgccca ttcagctgcc gggcgcgccg ccggtcatgg agtctgcgct cggtttctgg
     7321 gtggagcagg atggcgtgca gctgcacgtc atcggccgcg agcccttcgg tgggacgccc
     7381 gatccgaccc agaaccacgt ttcctggttc gtggaggatc tggatgtggc ggttggcgag
     7441 ctcgagcaga acggcatcga gatgcgggtg atgggcgagg gttcaggtcg gatcatctgg
     7501 gttctcgacc ccggcgggaa catcgtggag ttccagcagg acccggacct tgctcctccc
     7561 ccggcctgat cgtactggcg ggaagtcctc gtgaggttcg aaagccgccg ggagtcgttt
     7621 gccgagctcg agagcgccag gggattggtg cccggcatac ggacctcgca gtgcgagatc
     7681 gacaagcagc aggcgctgcc ggctgacctc gccgctcggc ttgccgctga cggcatgttc
     7741 cgactgcttc tgccggtctg tatcggcggt tccgggttgt cgttgccgcg gttcgtgcgc
     7801 gtggtggaag tgattgccga ggcggacggc agcacgggct ggtgcgtttc tcaggcggcg
     7861 gtgtttgcca gcgttgccga tctgctgcat gcggaaacgg ccgctgagat ctgggggacc
     7921 aaccgcgacg ctgccgttgc cacaggcatt ccgttcggtg ccgaggcccg agcggtcgat
     7981 ggcggctact tgctcgatgg ccgctggcgt tttgccagtg gctgcaccca tgccgactgg
     8041 tttgcacccc tggcctccgt ggttggcgat gtcgggagcg agccgtcgcg cacttttctc
     8101 gtcccccgcg aggaggttcg tctcggcgaa ggatggaacg tgcgcgggat gcaaggaacg
     8161 ggcagtcgtg agtaccggtt gcatgcgctg cacgtgccga gccggcgatc cgttgcggat
     8221 gcctacacca gtcatcccgg tgcctccacc gggctgcacg tcatgctcct gttcgcctgc
     8281 gggttcgcgg cggtggggct gggcgttgct cgacgggcag tggatgagtt catcgccctg
     8341 gccgccggaa agacgcccgt gtttgcgaac cggaagctgg ccgaggacga cctcgtgcag
     8401 agcgggctcg gcgaggcgga agcgctgctc ggcgccaccc gggctttcct gctggatgcc
     8461 gccgaggagt gcgagcatcg ccatgcaacc accggagccg tcgatttcga actcagatcc
     8521 cggatgcgcc ttgccgcgac ccacgccatg cgtcgctcgg cggacgtggt cgatcgggtt
     8581 tacgccatgg caggcacgga ctgcatcttc gacgaccacc cgctgcagcg ctgctttcag
     8641 gacattcacg cgctgaccca gcaggttcag ggcagggctt ctcactatcg ttcggtgggt
     8701 cgtgtgctgc ttggcaggca gaaagagaca ttcgctttct gaggacccag ggccctgctc
     8761 aaccgcgata gcctctccag cgctcgcgga tctccctggc gccatcccca tcgccctgag
     8821 ccgctcgggc gtctgcgatc agcagccagg catcgcgtac ccagtcgagg cgatcgccgg
     8881 ccaggctgat ggctttgttg gcgaactgca cggccgcttc cggatcctcg ttggcgagct
     8941 gcaagcgggc gagctgcagc cagaggtcgg cgctgcgggg attcaggcga atggccctct
     9001 ccacgtaggc gacggcggca tccgcgtcgc cggccgatgc ggcgcgctcg ctctgctcga
     9061 gcagcgccag ggtggccccg gccgtgtccg gctgccgggc ctcgggggtg ctcgccggtg
     9121 ggatcgggac ttcctgctgg ggcgccggct ccgcgtcctc gatgccgttg gtgtccacca
     9181 cgggaggcag cacggaggac gtggcgcaac ctgcagtcac gatcagcgtt gccaggcctg
     9241 ggatcagtcg tttcgtagcc aattgcgaat gcgctcaccc aaagacggcg gcttgatacc
     9301 acagcccggt ttttcctgta aagcagctcc ctcgggaacg ggtatggtga ccacgtccgc
     9361 gcagagcggg ttggccacga ggccggttgc gaattccacg tccaccttcc gcgaatccgg
     9421 atccggcgga atggcgatca cgtcgcgatc cgccatgatc cggtcccaga ccttgagcgc
     9481 gccggacgcg ccggtcatgc cggtcggcgc gttgtcgtcg cggcccaccc ataccactgt
     9541 cagcgcggcg tcatcgtatc cggcgaacca gctgtcacga tagtcgtctg aggtgcccgt
     9601 tttgcccgct acgccgaccc tggcgaagcg tgagctacgc cctgttccgc gcgccatgac
     9661 gacttgcagc gcccggtcga gggctcgcgc cgttttcgtg tcgatgcgct gctcgagctt
     9721 gagggggcgc ctcaacgtgg ggcttcggga ttcgtcgagc acgctgatca cggctttggg
     9781 cggctggtag aagccgccgc tggcgaagat gccgtagagc gccgatacct ccaccggcgt
     9841 cattgcctcc gcaccgagca gaagcgaagg atacgtgttt gcgggtgggc ggccgatgag
     9901 agtctcgaag cgggcggc
//
LOCUS       DBNHLM010000034         8810 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_362783, whole genome shotgun sequence.
ACCESSION   DBNHLM010000034 DBNHLM010000000
VERSION     DBNHLM010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8810)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8810
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_362783"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1268)
                     /locus_tag="AC16QF_01580"
     CDS             complement(138..1268)
                     /locus_tag="AC16QF_01580"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR6447271.1"
                     /translation="MQVTDLQYFISFHDDEFPLEDSLTIGRHLDNDVIVAGEDVLDYH
                     LRLEPTPRGPKVHPLGEASLILNGHHYSESRGLVAGDELVVGQNVLGVGVRRLHPAEA
                     ESWSLHGDAAHGVHEMVGDLSVGRGEDSGLVVADDHVSREHALFTLADDVVWVRDLDS
                     ANGTFVNGERVSGGCRLYHGDMIAFDQFEFQLIGQGADLTPVRAREGMQEGAMIKESG
                     PARSDTTEIAVVANADAVAELAPPAREKGTFLLGAAEPVLGITYRLRMGKTSLGRDDA
                     CDVVVKDRTVSSRHAEVVLRPEGATITNLMATNGTRVNGREIQSAQLADGDIIGLGRV
                     RFVYRSVTEGRRAARLRPLHRWLIAGAAIVAAVLAGLLIDTL"
     gene            complement(1255..3519)
                     /locus_tag="AC16QF_01585"
     CDS             complement(1255..3519)
                     /locus_tag="AC16QF_01585"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6447272.1"
                     /translation="MTLELPGFQVQAEIGHGATSRVYLAMHKQLSRLVALKILAEEYG
                     NDPAFRVRFLNESRIGSSLSHPNIVQVYDTGAHDNTLYLVLEYVRGGNLLQRVDRGMH
                     IRDLVRVVQNIGKALDYAHARGLVHRDVKPENILFREDGSAVLTDFGIAGFVDPDGPP
                     AKGGTVPGTPQYMSPEQAAGKALDGRSDLYSLGIVFFRVLTGDVPYKADTAVSLGIKH
                     LQEPIPRLPNHLAIFQDVVNRSLAKKPDQRFQSGQEFADALEALAASGQVPSLMIKAR
                     AVAPEEIRSLGSNVFARARDSGRGERQSAPERRPLTLGRAALAASLVAAVLAFGIVVV
                     EQPQWFTRMLAVTGIAEDPEVEDAWSNAQSLHQDPNQSLATIVAGYRRVVNLDPDHEG
                     ARAAIAGLASQWKSSIEDSLAAENLEQAGTKLEESLTVFPEDPSLVELTEQLTNRQHA
                     ESLLASTQALLRSHGLSDIPSATAAIQAYQEVLRLAPDHAVATEELNSLAQHYASLAA
                     EAADTGEVDAAISFLDRASAANSELRELDLVRSKIQQATTLQAAIIELLQQASAYRAA
                     GALINPPGENAAELYHRVLATYPENVIATQGLDEVVSQLRGTANQLLAAGDLGALQEL
                     VDRSSAVGLDPVAVNEMKSSLETEIARRATVAERLDQARGLLAEGFITEPQGRNVVAL
                     LREVERLDPGNEPAKRMLKESAERLASVAQEAYEVGMVDDAKHYLELALTVTPDVAAW
                     RELRATWEKSVASN"
     gene            complement(3519..4511)
                     /locus_tag="AC16QF_01590"
     CDS             complement(3519..4511)
                     /locus_tag="AC16QF_01590"
                     /inference="COORDINATES: protein motif:HMM:NF014543.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HIR6447273.1"
                     /translation="MQVAHHSTAARRLAFTLLFPALVLGSQALARTETIELANGDLYE
                     GEVVDDLRTGQGIYVWADGHRYEGQFEANRMHGQGVYFWPDGRTYTGSFVTDRREGRG
                     VLRWPNGDVYEGEFRDNKMHGTGRFVWANQDAYVGDFRDGSRSGQGQFEWSTGERYTG
                     GFDDGVRVGRGIFEWPDGRRYEGGFAGDAKSGKGVLEWPNGNRFVGDFAEDARSGLGI
                     FYWRDGTVYQGQYAQDKMHGYGIKEQPDGAMELQRWQEGALQFGKPLLADARCSLTID
                     SQPWMFYSTQCINGLAHGQGLAVSLDGRLIVEEGRFVLGNLIEGEIRVLRLGES"
     gene            4552..5355
                     /locus_tag="AC16QF_01595"
     CDS             4552..5355
                     /locus_tag="AC16QF_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR6447274.1"
                     /translation="MNHLFFFGSCVAVGALSGFLGGLLGIGGGIVIVPALIILFDTME
                     LFPQSIATPVAVGTSLAVIIFTSLAAARTQIRAGMVDWDVVRRWAPFLIIGSFLAGPI
                     AVALPVATFRTLIGAFLLFVSCVMLTNWKPDPHRKFPGAVAIAGIGMLGGLISGIAGV
                     GGGNVMVPTLVYFNKPVHRATATSSTLGVPIATAGTLGFVYTGWNAELGPALLGFVYL
                     PAFVAIVLATVVTAPMGVRVAYRVAPLPLRRGFGLLLVVIAARTLYSAF"
     gene            complement(5382..6785)
                     /gene="pyk"
                     /locus_tag="AC16QF_01600"
     CDS             complement(5382..6785)
                     /gene="pyk"
                     /locus_tag="AC16QF_01600"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR6447275.1"
                     /translation="MRRRRTKIICTIGPATANYPTLERLQAAGMNIVRLNMSHADHAS
                     AKKIINWIRTLNRKVQYPVAILLDTQGPEIRTGEINEPMDLSTGDVVTISVLPEDVER
                     SSIHVNYRELVDILQVGNRMTVDNGLLNFEVLEKKGQQLVCRVIDGGVLRSKSHVNLP
                     GVRVNLPSITPKDRRDVEFGIENDVDFIALSFVRSAEDVTELRGILGAKAKNIKIIAK
                     IEDQEGVTHVHDIVRAADGVMVARGDLGIETNIADLPNVQRRIVHSAAKWGKRSIVAT
                     HLLESMIENPIPTRAEVTDVANAIYEGVDAIMLSGETSVGKHPVRCVEQLDDIARSSE
                     RWPGLGYEKELVANTDKQHIANNAVALAEDIHAKGIVVITRRGITADLVTSARPRNVP
                     IYAFTNHSQTRRRMSLNRAVFGHRMQFSSDPETTLQRALKILRDREGFEPGDKVVVIS
                     DVLAEKNSDAIQLRHLD"
     gene            complement(6785..>8810)
                     /locus_tag="AC16QF_01605"
     CDS             complement(6785..>8810)
                     /locus_tag="AC16QF_01605"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR6447276.1"
                     /translation="AHWAQVKLYGGMLVEELDGASDSQWTLRLVYCHPDTLATREFDQ
                     QLSGHELATFLDETLDSYAGWVAMQLAHERNRNEAIARRDFPFPRYRPHQQAVARRVY
                     RALRDREQLLLEAPTGSGKSMALLFPAAKALAATDHQKIFYLTSRSTGSLAALDACER
                     LDPDGDYLRRVHIVARERACPVEGVPCDPALCQYANGYYDRIHGALAELLERPVMNAP
                     RVAEVAEAHRVCPHELSLDAAVWADVLVGDYNYVFDPMVRLQRFAEDPGIALLVDEAH
                     QLSTRARDMLSLEVTRSAVQQARVEQPPAAILRRVRSVERKLVEIASAQRRSGETSVP
                     RPDPLIRAMARLVDEVAGSDVDIAPYPATRSLLMTCYRWVRGESWYDEACCAHLGDAP
                     DGDYRLRRVHLDPAAYLKGVFDGFGGHVRFSGSVSPLPLYQRLHGTTGAPAERAGSPF
                     DPEQLEVLVVDDVPTYLAARARSLERLAALVETVVSANRGHYLVALPSFAYLDDLAWA
                     LAGRASLPDVAAQTPGMPPEERDRFLERFGSGSPACIGLVVMGGVFAESVDFAGRLAG
                     VICVGVGLPPPEPERARLQAHFAGVGEDGNAVAYQQPAMIKVLQMAGRLLRDPEDRGI
                     LCLVDDRFKDSAYGRFFPAHWRPQAVRAAQVAGRLEKFWQGAAGSPRLRA"
BASE COUNT         1457 a         2949 c         2810 g         1594 t
ORIGIN      
        1 gagggtgaac gggctgggat tctcggcgat gacgcggcgg attcccggcg cgatctcggc
       61 gacttcaccg taggtgaact ccagatcgcg tcgatacggt atgtgtacgc ccatgaggcg
      121 aagcataatc catcgcgcta gagggtgtcg atgagaagac ctgccaggac ggccgcaacg
      181 atggcggcgc ccgctatcag ccaacggtgc agcggacgca ggcgcgcagc gcgcctgccc
      241 tccgtgacgc tgcgatagac gaaacgcacc ctcccgagcc cgatgatgtc gccgtcggcg
      301 agctgggcgc tttggatctc gcgcccgttc acccgggttc cgttggtcgc catcaggttg
      361 gtgatggtgg cgccctccgg acgcaggacg acctccgcgt ggcgcgacga gaccgtgcga
      421 tccttgacca cgacatcgca ggcatcgtca cgcccgagcg acgtcttgcc catgcgcaga
      481 cggtaggtga tcccaaggac gggctctgcc gcgcccagca gaaacgtccc cttttcacgg
      541 gcgggcggcg ccagctcggc tacggcatcg gcgttggcaa cgacggcaat ctcggtggta
      601 tccgagcgag ccggcccgga ttccttgatc atggcccctt cctgcatgcc ttcgcgcgca
      661 cgcacggggg tcagatccgc cccctgcccg atgagctgaa actcgaactg atcgaacgcg
      721 atcatgtcgc cgtgatacag acggcagcct cccgagacgc gctcgccgtt gacgaaggtg
      781 ccgttggccg aatcgagatc ccgaacccaa accacgtcat cggccagcgt gaagagcgca
      841 tgctcgcggg agacgtggtc gtcggcgacg accaggccgc tgtcctcgcc gcgtcccacg
      901 ctcaggtctc cgaccatctc gtgaacgccg tgtgcggcat caccgtggag cgaccagctc
      961 tcggcttccg ccggatgcag gcgcctaacc cctaccccga gcacgttctg gccgacgacc
     1021 agctcgtcgc cggccaccag gccccgagat tcggagtaat gatgaccgtt gagaatcagg
     1081 cttgcctccc ccagcgggtg gactttcggc ccccgtgggg tgggttccag gcgcaggtgg
     1141 tagtcgagca cgtcctcacc ggcaacgatg acgtcgttgt cgagatgacg ccctatggtc
     1201 aggctgtctt cgagcggaaa ttcgtcgtca tgaaaagaaa taaaatattg cagatcagtt
     1261 acttgcaaca ctcttttccc acgtcgctcg aagctctcgc caggccgcga cgtccggcgt
     1321 caccgtcagc gccagctcga gatagtgctt ggcgtcgtcc accatgccca cttcgtaagc
     1381 ttcctgcgcc acgctggcaa gacgctcggc ggactccttg agcatgcgtt tggccggctc
     1441 gttgcccggg tcgagacgct cgacttcgcg cagcagagcg accacgttgc gtccctgggg
     1501 ctccgtgatg aatccctccg ccagcagccc gcgcgcttga tcgagccgct cggcgacggt
     1561 cgcgcgccga gcgatctccg tttcgaggct gctcttcatc tcgttgacgg ctaccgggtc
     1621 gaggcctacg gcgctggaac ggtcgacgag ctcctgcagc gcccccaggt cacccgcggc
     1681 aagcagctga ttggccgtcc cgcgcagctg ggaaaccacc tcgtcgagcc cctgtgtggc
     1741 gatgacgttc tcggggtacg ttgcgagcac ccgatgatag agctcggccg cgttctcgcc
     1801 cggggggttg atgagcgcgc cggccgcccg ataggcgctg gcctgctgca gcagctcgat
     1861 gatggctgcc tggagcgtcg tcgcctgctg aatcttcgat cgaaccaggt ccagctcacg
     1921 cagctccgag ttggcggctg acgcccgatc gaggaagctg atcgccgcat ccacctcgcc
     1981 cgtatccgcc gcttccgcgg ccagcgaagc atagtgctgt gcaagggagt tgagctcctc
     2041 cgtggcaacg gcgtggtcgg gcgcgagccg cagaacctcc tgataggcct ggatggcagc
     2101 cgttgcagag ggaatgtcgg agagcccgtg actgcgcagc agagcctggg tgctggcgag
     2161 caggctttcc gcgtgctgcc ggttcgtcag ctgctcggtg agctcgacga gcgaaggatc
     2221 ctcgggaaaa accgtcagcg actcttcgag cttcgtaccc gcctgctcga gattctccgc
     2281 cgcaagagaa tcctcgatgc tgctcttcca ctggctggcc agcccggcaa tggcggcgcg
     2341 agcgccctcg tggtcgggat ccagattcac cacccgccgg taaccggcaa cgatggtcgc
     2401 caggctctga ttcggatcct ggtgcagaga ctgggcgttg ctccaggcat cttccacctc
     2461 gggatcctcg gcgatgcccg tgacggcaag catgcgggtg aaccactgag gctgctcgac
     2521 gacgacgatg ccaaaggcga ggaccgccgc taccagcgac gccgccagag ccgctctgcc
     2581 tagggtgagg gggcgccgct ccggcgcgga ttgacgctcg ccccgcccgg agtcccgggc
     2641 ccgggcaaag acgttcgatc ccagggagcg gatctcttcc ggcgccacgg cacgggcctt
     2701 gatcatgagg ctgggcacct gtcccgacgc cgccagggct tcgagggcgt cggcaaactc
     2761 ctgaccgctc tggaagcgct ggtcgggctt tttcgccagg ctgcggttga cgacgtcctg
     2821 gaagatggcc aggtggttgg gaagccgcgg gatcggttcc tgcagatgct tgattccaag
     2881 actcaccgcg gtatcggcct tgtagggaac gtccccggtc agaacacgga aaaaaacgat
     2941 tcccaggctg tacagatccg accggccatc cagcgccttg ccggcggcct gctcggggct
     3001 catgtactgc ggcgtccccg gtacggtgcc gcccttggcc ggcgggccgt ccggatcgac
     3061 gaacccggcg atgccgaagt cggtgagcac ggcgctgccg tcctcgcgga aaagaatgtt
     3121 ctccggcttc acgtcccggt ggacgagccc tcgggcatgg gcgtaatcca gcgctttgcc
     3181 gatgttctgc accacgcgaa ccagatcacg gatgtgcatg ccccggtcca cgcgctgcag
     3241 aagattcccg ccccgaacgt attcgaggac caggtagaga gtgttgtcgt gggcgcccgt
     3301 atcgtaaacc tggacgatgt tcggatggga caggctggat ccgatgcgcg actcgttcag
     3361 aaagcgaacg cggaaggcgg gatcgttacc gtattcctcc gcgaggatct tgagcgccac
     3421 caggcggctc aactgcttgt gcatggcaag atagactcgg gaggtggccc cgtgaccgat
     3481 ctccgcctgc acctgaaatc cgggaagctc caacgtcact acgactctcc cagtctgagg
     3541 acccggatct ccccctcgat caggttgccg agcacgaacc tgccctcctc gacgatcagc
     3601 cgcccgtcca ggctgaccgc cagaccctgg ccgtgagcga gcccgttgat gcactgcgtg
     3661 ctgtagaaca tccatggttg cgagtcgatg gtgagcgagc aacgcgcatc ggccagcaga
     3721 ggcttgccga actgaagcgc gccctcctgc cagcgctgca gttccatggc gccgtcaggc
     3781 tgctccttga tgccgtagcc gtgcatcttg tcctgggcgt actggccctg gtagacggtg
     3841 ccgtcacgcc agtagaagat ccccagtccg gaacgggcat cttccgcgaa atctccgacg
     3901 aaccggtttc cgttcggcca ctccaatacg cccttgccgg acttcgcatc accagcaaag
     3961 ccgccctcgt atcgacgccc gtcgggccac tcgaagatcc cacggcctac ccggacgcca
     4021 tcgtcgaagc cgcccgtgta tcgctcgccc gtgctccact cgaactggcc ttgccccgag
     4081 cgggacccgt cgcggaagtc gccgacgtag gcgtcctggt tggcccacac gaaccggccc
     4141 gtgccgtgca tcttgttgtc gcggaactcg ccctcgtaga cgtcgccgtt aggccagcgc
     4201 agcacgcctc ggccctcccg acggtctgtt acgaaggatc ccgtgtaggt gcgtccatcc
     4261 ggccagaagt agacgccctg accatgcatg cggttggctt cgaactgccc ttcgtagcgg
     4321 tgcccgtcgg cccagacgta gatcccctga cccgtgcgca gatcgtcgac cacctctcct
     4381 tcgtaaagat caccgttggc gagctcgatg gtttcggtac gggcaagcgc ctggctgccc
     4441 agaaccagcg ccgggaaaag cagggtgaac gcgagccgtc gtgcggcggt ggaatggtga
     4501 gctacctgca agttcagtag actcccgaga tcgtcgagta cgttacttta attgaatcat
     4561 cttttcttct ttggttcctg cgtcgcagtt ggcgccttga gcggctttct cggcggtctc
     4621 ctgggcatcg gcggcggcat cgtcatcgtc cccgccctca tcatcctgtt cgataccatg
     4681 gagctctttc ctcagagcat tgccacgcct gtggccgtgg gaacctccct cgccgtgatc
     4741 atcttcacgt ccctcgcagc ggcgcgaacc cagatacggg ctggcatggt ggactgggac
     4801 gtggtccggc gatgggcgcc ctttctgatc atcggcagct tccttgcggg acccatcgcg
     4861 gtcgcccttc cggtggccac cttccgcacc ctgatcgggg catttctcct cttcgtatcc
     4921 tgcgtgatgc tcacgaactg gaagccggat ccgcaccgga aatttcccgg ggccgtggcg
     4981 atcgcgggca tcggcatgct gggcggcctc atctccggga ttgccggtgt cggcgggggg
     5041 aacgtgatgg tgcccacgct cgtctacttc aacaaacccg tgcacagggc caccgcgacg
     5101 tcgagcaccc tcggcgtgcc catcgccacc gccggcaccc tgggtttcgt ctacaccggc
     5161 tggaatgccg agcttggccc cgccctgctc ggcttcgtgt atctgccggc gttcgtggcc
     5221 atcgtgctcg ccaccgtcgt gacggcgcca atgggcgtgc gcgtcgccta tcgggtcgcg
     5281 ccactgccat tgcgcagagg attcggcctg cttctggtgg tcatcgcggc gcggacgctc
     5341 tactcggcgt tctgacacct gccagcggcg gccgtctgga ttcagtccag gtgccgcagc
     5401 tgaatggcgt cggagttctt ctctgccagc acgtccgaga tgacgacgac cttgtcgcca
     5461 ggctcgaacc cctcacgatc ccgcaggatt ttcagcgccc tctgcagcgt ggtctcggga
     5521 tcgctggaga actgcatgcg atggccgaag acggcccgat tcagcgacat gcggcgccgc
     5581 gtctggctgt ggttggtgaa cgcgtagatg ggcacgtttc gcggccgcgc gctcgtgacg
     5641 agatcggcgg tgatgccgcg ccgggtaatg accacgatgc ctttggcatg aatgtcctcg
     5701 gccagcgcta ccgcgttgtt ggcgatgtgc tgcttgtcgg tgttggccac gagttccttt
     5761 tcgtatccga gcccgggcca acgctctgat gaacgggcga tgtcgtcgag ctgctccacg
     5821 cagcgcaccg ggtgcttgcc cacgctggtc tcgccggaga gcatgatggc atcgacgccc
     5881 tcgtagatgg cgttggcgac gtcggtcacc tcggcccggg tgggaatggg gttttcgatc
     5941 atcgactcca gcaggtgcgt cgccacgatg gagcgtttgc cccactttgc cgccgagtgc
     6001 acgatgcgcc gctgcacgtt cggcagatcg gcgatgttcg tctcgatgcc gagatcgccc
     6061 ctggccacca tcaccccatc ggccgcgcgc acgatgtcgt gcacatgggt caccccttcc
     6121 tgatcttcga tcttcgcgat gatctttatg ttcttcgcct tcgcgcccag gatgccgcgc
     6181 agctcggtga cgtcctccgc cgagcgcacg aaggacagcg cgatgaagtc cacgtcgttc
     6241 tcgatgccga actccacgtc ccggcgatcc ttgggcgtga tcgatggcag gttcacacgc
     6301 acccccggca ggttgacgtg agatttgctc cgcagaacgc ccccgtctat cacccggcag
     6361 acgagctgct gacctttctt ctccagcacc tcgaagttga gcagaccgtt gtcgacggtc
     6421 atccggttgc ccacctgcaa aatgtccacg agttcccgat agttgacgtg aatggagctg
     6481 cgctcgacgt cctcgggcag cacgctgatg gtcacgacgt cgccggtgct cagatccatg
     6541 ggttcgttga tctcgccggt gcggatctcg gggccctggg tatcgagcag gatggcaacc
     6601 ggatactgca ccttgcggtt gagggtgcgg atccagttga tgatcttctt tgccgaggcg
     6661 tggtccgcat gggacatgtt cagacggacg atgttcattc ccgccgcctg cagcctttcc
     6721 agcgtggggt agttggccgt ggctggaccg atggtgcaga tgatcttggt acgtcgtcgt
     6781 ctcatcacgc gcgcagtcta ggggagccgg ccgccccctg ccagaacttc tcgaggcggc
     6841 cggcgacctg ggcggccctc accgcttggg ggcgccagtg agcgggaaag aatcggccgt
     6901 aggccgaatc tttgaagcgg tcgtctacca ggcagagaat gccccggtct tccggatcac
     6961 gcagcaggcg tccggccatc tgcaggacct tgatcatcgc cggctgctga taggccacgg
     7021 cattgccgtc ctcaccgacg ccggcgaaat gcgcctgaag ccgagcccgc tcgggctcgg
     7081 gcggaggcag ccccacgccc acgcagatga ctccggccag gcgtccggca aagtccacgg
     7141 actcggcgaa aacgcctccc atcacgacga ggccgatgca ggcgggcgaa ccggatccga
     7201 acctctccag aaatcggtct cgctcctccg gtggcattcc cggcgtctgc gctgcgacgt
     7261 cgggaagcga tgcccgaccg gccagggccc aggcaagatc gtccaggtac gcgaacgacg
     7321 gcagcgcgac cagatagtgg ccgcgattcg cggaaaccac cgtttccacg agcgctgcca
     7381 ggcgctcgag gctccgtgcc ctcgccgcca agtaagtcgg cacgtcgtcc accactagca
     7441 cctcgagctg ctcgggatcg aaggggctgc cggcacgttc cgccggcgcc ccggtggtcc
     7501 cgtgaaggcg ctggtagagc ggcagaggag acacgcttcc cgagaagcgg acgtggcctc
     7561 cgaaaccgtc gaaaacgcct ttcaggtagg cggccggatc caggtgaacc cggcgcaggc
     7621 ggtagtcccc gtccggcgcg tctcccaggt gcgcgcagca cgcctcgtcg taccaggatt
     7681 caccacgcac ccatcgataa cacgtcatca gcagagaccg agtggccgga tacggcgcga
     7741 tgtcgacgtc tgatcccgcc acctcgtcga cgaggcgcgc catggcgcga atcaacgggt
     7801 cgggacgggg aacggacgtt tcgcccgagc gtcgttgggc gctcgcgatc tcgacaagct
     7861 tgcgctccac ggaccgcacg cggcgcagga tggcggccgg cggctgctcg acccgcgcct
     7921 gctgcacggc gctgcgcgtc acctccaatg acagcatgtc ccgggcacgc gtgctcagct
     7981 gatgggcctc gtccaccagc agagcgatgc cgggatcctc ggcgaaccgc tgcagacgca
     8041 ccatggggtc gaagacgtag ttgtagtcac cgaccagcac gtccgcccag acggccgcat
     8101 ccagtgacag ctcgtgggga cacacccgat gggcttcggc cacctccgcc accctcggtg
     8161 cgttcatgac gggccgctcc agcaactcgg cgagcgctcc gtggattctg tcgtaatagc
     8221 cgttcgcgta ctggcacagg gccggatcac agggcacgcc ctccaccgga caggcgcgtt
     8281 cccgcgcgac gatgtgcacg cggcgcaggt aatctccgtc cgggtcgagc ctctcacaag
     8341 catccagtgc ggccaaggac ccggtagagc ggctggtgag atagaagatc ttctggtgat
     8401 cggtagcggc aagcgctttg gctgcgggaa acaggagcgc catgctcttg ccgctccccg
     8461 taggcgcttc caggaggagc tgttcacggt cgcgcaacgc ccgatacacc cgccgggcca
     8521 ccgcctgctg atgggggcga tagcggggga acgggaagtc gcgacgcgcg atcgcctcgt
     8581 tccggttccg ctcgtgggcg agctgcatcg ccacccatcc ggcgtagctg tcgagggtct
     8641 catcgaggaa cgtggcgagc tcatgcccgg acaattgctg atcgaactcg cgggtggcga
     8701 gcgtgtcggg atggcagtac accagccgca acgtccattg gctgtccgat gcgccatcga
     8761 gttcctccac cagcatcccg ccgtaaagct tgacctgcgc ccaatgggcg
//
LOCUS       DBNHLM010000035         4821 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_4414, whole genome shotgun sequence.
ACCESSION   DBNHLM010000035 DBNHLM010000000
VERSION     DBNHLM010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_4414"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="AC16QF_01610"
     CDS             complement(<1..872)
                     /locus_tag="AC16QF_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR6447277.1"
                     /translation="MYSTGTTPAAAITLGITLICVVWWVFEPIPIPATSVLPLAMLPL
                     LGVLTPQQVAQAYGHPLILLMLGGFILSTALARNGAHRRIALTMVRAFGGSSSRRLVF
                     GFMAASAVLSMWISNTATTLMLLPVALAVLEKAEDPKLATPLLLGIAYAASIGGIGTP
                     IGTPPNVVFMGVYNDLGVGQIGFADWMSFAMPVVLVLLPIAAVWLTRRLGHQGTVDVP
                     EVGAWQAGEVRVLMVFALTAVAWITRSEPFGGWTALLGLAQTNDAMVALLAVVIMFVV
                     PDGKGERLLDWETAT"
     gene            969..2390
                     /gene="sbcB"
                     /locus_tag="AC16QF_01615"
     CDS             969..2390
                     /gene="sbcB"
                     /locus_tag="AC16QF_01615"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HIR6447278.1"
                     /translation="MAASYYWYDLETSGIEPRWDRVIQFAGLRTDLSLNPVGDEYCTY
                     VELPDDVLPSPEATLVTGITPDVTRREGVSEWQAMTRINELFSAPQTCVAGYNSLRFD
                     DEFIRFGFYRMLIDPYAREWQNGNSRWDIIDLVRATGALRRDGIRWPTDDEGLPVYRL
                     ELLTKANDLEHGQAHDAMSDVRATVALARLIRDCQPKLFDYYFNGRSKKQVKQLLEPF
                     GARVCLHVSGMYPRSRYCAAPVVSLCRHPKNSNSIIVADLSEDVEPLIGWTDEQIRAE
                     LFKEGSTSRPPLKEIRINRCPFVAGIEVMTGENWERIGFDRREIKERQRRLARPGLAQ
                     KIMRVYGDRPPPAAPDVEAALYDAFLQDADRGRCASLQEALGQGKWLDLDYSDRRLVS
                     LSTRLKSRSFAESLSEEERQDWHEFVRDKLNAEDAPWLTLKEFDARLDRIRQDSAGED
                     ARRERLLGKLEAYAGGLKARYGG"
     gene            complement(2470..3462)
                     /locus_tag="AC16QF_01620"
     CDS             complement(2470..3462)
                     /locus_tag="AC16QF_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6447279.1"
                     /translation="MSLSPYHHPTTICLVDDNQSFLDSMILEMPSMWACRAFTDPHAA
                     AEFLNQPIGLPPLAERCLSRETSTGSDAVIHIDLGLIEQEVNHPDRFRRISVLVADYA
                     MPSMNGLELCAAARDPHLRKAMLTGVADEKLAVEAFNEGLIHRFIPKHGTSATAIARE
                     YIAELQAEYFEQYTALLQGSLTVGKPGFLTDPDVSQWVEALMRTERLVEYYLVDDPPG
                     LLMLGSDGAMFRAMILDPVELSHQLEIARRYGAPDHIVKGIAERRLAVLLAGDSPADY
                     YGAESFPWEENVCAVSPVGDDGKWFVGVVRDAPVDIDFDPRLSSYDAYLATLER"
     gene            complement(3486..3782)
                     /locus_tag="AC16QF_01625"
     CDS             complement(3486..3782)
                     /locus_tag="AC16QF_01625"
                     /inference="COORDINATES: protein motif:HMM:NF015807.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HIR6447280.1"
                     /translation="MKEKYYKVLVRLPPEMREMLAESARHYRRSRNSDIVARLQESFR
                     GIADSARGRGLAPPMHDQFEQLFRADLSPQEQSLVTAFRRLSSDQQTALLALLN"
     gene            3871..4626
                     /locus_tag="AC16QF_01630"
     CDS             3871..4626
                     /locus_tag="AC16QF_01630"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6447281.1"
                     /translation="MQPVRKDNIRLAATILLVRDVGGGPEVFMVQRPGGVDFPDLHVF
                     PGGKVDAADFVPELVTDLDDRSASQRLGVEAGGLRYWVAAIRECFEECGVLLADRDGE
                     LFTPRTDAEAERFAGHRHALISGELDFAAMCRQEGLAIRAGRAHYFSHWITPEVAPRR
                     FDTRFFIAHMPTGQVTAGHAWETAAEAWVAPGRALELFETGRWQMIAPTLTTLQTIAR
                     YSGVAHMLADVAAEAHLDELTADLRHQGMYPLR"
     gene            4637..>4821
                     /locus_tag="AC16QF_01635"
     CDS             4637..>4821
                     /locus_tag="AC16QF_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6447282.1"
                     /translation="MNPVLSVAGLAKRYVDGSAERVVLAEVSLEVGPGEILAVCGPSG
                     SGKSTLLSIVAGLVLPDA"
BASE COUNT          843 a         1529 c         1634 g          815 t
ORIGIN      
        1 gtagcggtct cccagtcgag caaccgctcg cccttcccgt caggcacgac gaacatgatc
       61 accacggcga gcagggcgac catggcgtcg ttggtctggg ccagccccag cagtgccgtc
      121 cagcctccga acggctcgct gcgggttatc caggcgacgg cagtcagggc gaagaccatc
      181 agcacccgaa cctcaccggc ctgccacgcg cccacctcgg ggacgtccac ggtgccctgg
      241 tgtcccagtc gacgggtcag ccacacggcg gcgatgggca ggagcaccag caccacgggc
      301 atcgcaaacg acatccagtc cgcaaacccg atctgaccca cgccgagatc gttgtacacg
      361 cccatgaaca cgacgttggg cggggttccg atgggcgtgc cgatgccgcc gatgctggcg
      421 gcgtaagcga tgcccagcag gagcggcgtg gcaagcttcg gatcctcggc cttttccagc
      481 accgcgagcg ccacgggcag aagcatcagc gtggtcgcgg tgttggagat ccacatgctc
      541 agcaccgcgc tggcggccat gaacccgaaa accagtcgcc ggctgctgct gccgccaaaa
      601 gcgcggacca tggtcagcgc gatgcgccgg tgggccccgt tgcgggccag ggctgtggag
      661 aggatgaacc cgccgagcat gagcaggatg agcggatgac cgtaagcctg ggcgacctgc
      721 tgcggcgtca gcacccccag caggggcagc atggccaggg gaagcaccga cgttgccggg
      781 atggggatag gctcgaacac ccaccacacc acgcagatca acgttatgcc caaggttatg
      841 gccgccgccg gggtcgtccc ggtagagtac atgaggtatc cgacgaacaa tgcgagaatg
      901 gggccagcgg ggagcgcgat gcgctttgcg ttggaagccg acatgggaag gcaaggttat
      961 aggaagcgat ggcagccagc tactattggt acgaccttga aacctcgggt atagagccgc
     1021 gctgggatcg cgtgatccag ttcgcagggc tgcgcaccga tctgtcgctc aacccggtcg
     1081 gggacgagta ctgcacctac gtggagctcc ccgacgacgt gctgccgagt cccgaggcga
     1141 cgctggtgac gggcattacc cctgatgtca cccgccggga aggcgtcagc gagtggcagg
     1201 cgatgacgcg catcaacgag cttttctcgg cgccccagac ctgcgtggcc ggctacaaca
     1261 gccttcgctt cgatgacgag ttcattcgct tcggcttcta ccggatgctc atcgacccgt
     1321 acgcccggga gtggcagaac ggcaactcgc gctgggacat catcgatctc gtccgggcga
     1381 cgggcgcgct gcggcgcgac gggatccgct ggcccaccga cgacgaaggt cttcccgtct
     1441 accggctcga gctgctcacc aaggccaatg acctcgagca cggccaggcc cacgatgcca
     1501 tgtcggacgt gcgcgctacg gttgcgcttg cgcggttgat tcgcgactgc cagccgaagc
     1561 tgttcgacta ctatttcaac ggccggtcga agaagcaggt caagcagctg ttggaaccgt
     1621 tcggagcgcg cgtatgcctg catgtctcgg gcatgtatcc gcgcagccgc tactgcgccg
     1681 cgccggtggt gtcgctgtgc cgccacccca agaacagcaa ctccatcatc gtcgccgacc
     1741 tcagtgaaga cgtggagccg ctgatcggct ggaccgacga gcagatacgt gcggagctgt
     1801 tcaaggaggg ttccaccagt cgccccccgc tgaaggagat ccgcatcaat cgctgcccgt
     1861 tcgtggcggg catcgaggtc atgaccggcg agaactggga gcgcatcggg ttcgaccgtc
     1921 gagagatcaa ggaacgccag cggcgtctcg cccggcccgg actggctcag aagatcatgc
     1981 gcgtatacgg cgatcgcccc ccaccggcag cacccgatgt ggaagcggcg ctttacgacg
     2041 cctttctcca ggatgcggat cgcggtcgct gcgccagcct tcaggaggcg cttggccagg
     2101 ggaagtggtt ggacctcgac tattcggacc gccggctggt ttcgctctcg acccggctga
     2161 agtcccgcag ctttgcggag tcgctctccg aggaggagcg gcaggactgg cacgagttcg
     2221 tgcgcgacaa gctcaatgct gaggatgcgc cgtggctcac gctgaaggag ttcgacgcgc
     2281 ggctggatcg gatccggcag gactcggccg gagaggacgc ccgacgcgag aggctgctcg
     2341 ggaagctcga agcctacgcc ggggggctga aagcacgcta cggcggctga cacggtccaa
     2401 ggccgtcccg gcggcccgga ccgaggtgcc agccggtcgg gtagcgccgg ccccagtcgc
     2461 gatgccgctt cagcgctcca gggttgccag gtaggcgtcg taggaggaaa gcctcgggtc
     2521 gaaatcgatg tctaccggcg cgtctcgcac cacgccgaca aaccacttcc cgtcatcgcc
     2581 cacggggctg accgcgcaga cgttctcttc ccacggaaag ctctcggcgc cgtaatagtc
     2641 ggccggggag tcgccggcga gcagcacggc cagccggcgc tccgcgatgc ccttcacgat
     2701 gtggtcgggc gcgccatacc ggcgcgcgat ctcgagctga tggctcagtt caacggggtc
     2761 caggatcatc gctctgaaca tcgcgccgtc gctgccgagc atcagcaggc ccggcgggtc
     2821 atccaccagg tagtactcga ccagccgctc ggtgcgcatg agtgcctcga cccattgcga
     2881 gacgtcggga tcggtcagaa agccgggctt gcccacggtc aggctgccct gaagcagggc
     2941 cgtgtactgt tcgaaatatt cggcctgcag ctcggcgatg tattcccggg cgatggcggt
     3001 cgccgacgtc ccgtgtttgg gaatgaaccg gtggatgaga ccctcgttga aggcctcgac
     3061 tgccagcttt tcatccgcga cgccggtgag catggccttg cgcagatggg gatccctggc
     3121 tgcggcgcaa agctccagcc cgttcatgga tggcatggcg taatcggcca ccagcaccga
     3181 tatgcgcctg aaccggtccg gatgattgac ctcctgctcg atgaggccga ggtcgatgtg
     3241 aatgaccgcg tcggagcccg ttgaggtctc ccgggaaagg cagcgctcag ccagcggcgg
     3301 cagcccgatg ggttggttca ggaactctgc agctgcgtgg gggtccgtga acgcccggca
     3361 agcccacatg ctgggcatct ccaggatcat gctgtccaga aaggattgat tgtcgtccac
     3421 caggcagatc gtggtggggt ggtgataggg tgaaagtgac atcaggggtg tcccggtccg
     3481 cagcgtcagt tgagcagggc gagcagcgcc gtctgctggt cactgctcag ccggcgaaag
     3541 gccgtgacga ggctctgttc ctgcgggctg aggtcggccc tgaacagctg ctcgaactgg
     3601 tcgtgcatgg gtggcgcaag tccccgtccc cgggcggaat ctgctatgcc tctgaaggac
     3661 tcctgcagac gcgctacaat gtcggagttc ctgctgcgcc ggtagtgtcg cgccgactct
     3721 gcgagcatct ctcgcatttc cggcggaaga cgcacgagga ccttgtaata tttttctttc
     3781 attttgaggt ctcggccgcg tggtcggtct cagactagcg cggcatgccg agcccgacaa
     3841 gagatggcaa tttgatatca gttcctgcaa atgcagcccg ttagaaaaga caacatcagg
     3901 ctcgccgcga ccatcctgct cgtccgcgac gttggcggcg gccccgaggt gttcatggtt
     3961 cagcgtcccg gcggcgtcga cttcccggac ctgcacgtct ttccgggggg caaggtggac
     4021 gcggccgact tcgtgcccga gcttgtcacc gacctggacg accggtcggc gtcgcagcgg
     4081 ttgggcgtgg aggcgggcgg cctgcgatac tgggtggcgg ccattcgcga gtgcttcgag
     4141 gagtgcggcg tgcttctggc ggatcgcgac ggtgagctct tcaccccgcg aacggatgcc
     4201 gaagcggagc gctttgccgg acaccgtcac gcgctcatct ccggcgagct ggatttcgcg
     4261 gccatgtgcc ggcaggaagg gctcgccatc cgggccggcc gcgcgcacta cttcagtcac
     4321 tggatcacgc ctgaggtggc gccgcggcgc ttcgacacgc ggttcttcat cgcccatatg
     4381 ccaacgggcc aggtcaccgc tgggcacgct tgggaaacgg cggccgaggc ctgggtagcg
     4441 ccaggcaggg ccctggagct tttcgagacg gggcgatggc agatgatcgc gcccacgctg
     4501 acgaccctgc agaccatcgc gcgttattca ggcgttgcgc acatgctggc cgatgtggcc
     4561 gcggaggcgc acctcgacga gctgacggcc gatctgcgcc atcaggggat gtatccactg
     4621 cgatgagggc agaatcatga acccggtcct gtccgttgcc ggccttgcca aacgctacgt
     4681 tgacggaagc gccgagcggg ttgtgctcgc ggaggtctcc ttagaggtgg gtccgggaga
     4741 gattctggcc gtttgcgggc ccagcggctc cggcaagtcc actctgctga gcatcgtcgc
     4801 cggactggtg ctgccggatg c
//
LOCUS       DBNHLM010000036        17904 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_662798, whole genome shotgun sequence.
ACCESSION   DBNHLM010000036 DBNHLM010000000
VERSION     DBNHLM010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 17904)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17904)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17904
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_662798"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="AC16QF_01640"
     CDS             <1..325
                     /locus_tag="AC16QF_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HIR6447283.1"
                     /translation="MVTPVFETMGVAATLNLAANLICLRILFPFRNDDLNMTSVWECS
                     RNDIFDGLAVLTATVAVWVSDSAWPDLLIAIALLIVFLRSAARILSRSWRELHAAPTP
                     GPGTG"
     gene            complement(359..1462)
                     /locus_tag="AC16QF_01645"
     CDS             complement(359..1462)
                     /locus_tag="AC16QF_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6447284.1"
                     /translation="MAGIPETPEDFTTSWLCEVLGVPESEVLDVDVRTSEADSGFVSL
                     ACFLELETRPGSGLPRQLVGKLAPTFEGARKLSREFGLFAREKQFYSEIAPLVAIRSP
                     GAYHCDYDAELGTGVLLLEDCSHMRAPVQTDEQPTTLAELELIVDAAADMAISSWNAD
                     WLATKDELMGPDHPAFVGYFSGIQEGYKDFVDSEFMSWAPDGFEGLAVRLGENMRTIA
                     AAQPETNLSLCHMDLRLANVFLDEESDDPVVLYDWQSMYPGRTAQDLGYLITSGYTPA
                     FRREHERNLVSRYHQRLVDAGIPGYSFDEAWFDYRHGIIIGLRLLPMALGDLDVSTEG
                     GQAIFRKIIRNFPEAALDHGGVELVDEILARRA"
     gene            1612..2391
                     /locus_tag="AC16QF_01650"
     CDS             1612..2391
                     /locus_tag="AC16QF_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447285.1"
                     /translation="MKTINRTLGRSLLVLIPFLALNGMFAYADSHGPALPSGVLEVFQ
                     CNYNSGKDRDDLNALRDFYLKQAKKVNYTPPDAFLWTMTNGSWPTTLTWVNVHEDLSA
                     YSRSYDTIAASTDLAAVNERAETVMTCVPALGSYAHAFIRDPDAQGPWEATVASYACD
                     LRPEQDMSDLEDLMEHVATVNQSMGDDAMDELLHFIPMTGLNNQPDVLVFAASRRGAS
                     SWSSHLALLEQSGAGRAVLAHFDSVLDCRLSLWDVEQMVGG"
     gene            complement(2412..5390)
                     /locus_tag="AC16QF_01655"
     CDS             complement(2412..5390)
                     /locus_tag="AC16QF_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6447286.1"
                     /translation="MARGIPISAMVLMMPCALQAQERLVIEEVIVTAQRVEESAQKVP
                     IALNAFDETGIEDRQIIGIGDLSLFVPNLSYNTNNVGDTHVTIRGVGSLVSRRDSESG
                     VSLHVNQVPLPSGQPPFEIYDLARIEVLRGPQGTLYGRNATGGVINLITNRPSFDGTS
                     GYLDVEAGNHDLLRLRGALNAAVADRLALRLAGLSLDRDGYTDNLAGGQVPGVASEID
                     GRDLYSVRASATWRVTEATEIWGLYERFDEDDDRMLTHNRRCKTALRPTTRTGCEPGE
                     WGLEPNNPNQARFQTSITNGLRGVIPLGARDAETGLTFAYPRPEITNLRDVHIDFAPV
                     YEFEQNLWQLGASHDFDWATLSFIGSYQDWRLRTLQDNDWSVGHELAPIPENPSGLYP
                     VSALPEGKNPLDGPECNVNEAQFGVLGGCVLDPNLTRQFWYGERREWRDFYSAELRLR
                     SDLDGRVNFLIGGNYQFSDDHAVSGGGTNDYWEMWTRFGRAQAPATGPNPFALNLNAL
                     DRLMVLAGGEDKNEFESFSALSEIYVDLTDHLKLTVAARYNRDEKRYRARGIGAPFDI
                     NTGLGGVFGRDVWVRPQAALYIFGISDDSGLLDFYGLTEEVDAALATGGFPGLLAANL
                     IASQIPILTAWNEGAIIAGVPTDFEWDAVSGRAVLDWQITPDMLAYASFARGYKPGGL
                     NGFFSTNPTYDREDVNSFELGFKSLLADDSLMLNVAAFFNDYEGLQLTNLAAEQFTRN
                     IENTNVDADMYGAEIEARWRPPFAPRAELEIGYSWLNAELKNEPPRIDALWLTNGDPE
                     YVELLSFATNAGSPNGRYVARVEDVLPWVNHAIAIGAAIGPDEAPATIYPNGIPAWFD
                     GAFLSAVGVELLPGIPVPVSGNSVPDTPEHTVHLGVSYTWDVPAGALTARWDYYWQSK
                     SYLTIFNRSAETIDAWDQHNLSLIYETGDGRWSARAWVRNIENDLHILGGYRQHSQQD
                     FSVSEPRAFGVSLRFNFAPG"
     gene            complement(5554..5847)
                     /locus_tag="AC16QF_01660"
     CDS             complement(5554..5847)
                     /locus_tag="AC16QF_01660"
                     /inference="COORDINATES: protein motif:HMM:NF020873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TehB family protein"
                     /protein_id="HIR6447287.1"
                     /translation="MAFTLHMRALPENVLPYRRTATFHQDSIPPGLLKAHSTKPGTWG
                     RIVVVTGELLYRILEPDPAEFLLSPHIMGVVEPTVLHEVAPVGEVEFYIEFLR"
     gene            complement(5934..6701)
                     /locus_tag="AC16QF_01665"
     CDS             complement(5934..6701)
                     /locus_tag="AC16QF_01665"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF048554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6447288.1"
                     /translation="MDLGLNGAKAVISGGTQGIGLAVAKQLMVEGVAVSIAARRQEGV
                     DAALEELSALGTVVGQVCDVADYDATVAWMQNAADALGGIDIVVHNATASGQPGPGPQ
                     SWINNFSVDVLGMVGMSEGALPFLEQSERAALVQIASITGIEHHDVPISPSYGAMKAA
                     SIRHMAQLAQQWGPKGIRANTVAPGPIFIENGVWNGIKDNAPALYERDRDYHPTKRMG
                     TGEEVARVVAFLASPAASWVNGTTVRVDGAFTRGVNF"
     gene            complement(6828..7355)
                     /locus_tag="AC16QF_01670"
     CDS             complement(6828..7355)
                     /locus_tag="AC16QF_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6447289.1"
                     /translation="MSKEFNYDDVSTYTLDKADELALIAAQNECTFMWSTKDGWPVGV
                     IMTYVYAEDCFWLSVSSLRVRVKAVQRDPRASISITSKGTPLRSGQSLTYKGTCEVFS
                     DQATVDWFLPELAKALRIGDERARRVFVELNNTENRRVIKFTPTKAIGFDGRKMGKAT
                     MEATGGDPVVTKRSS"
     gene            7549..8898
                     /locus_tag="AC16QF_01675"
     CDS             7549..8898
                     /locus_tag="AC16QF_01675"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006720 - isoprenoid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6447290.1"
                     /translation="MAAVIEEIPSHLRGNGRPITEERTLTDLKVTGAIPPELDGRYVR
                     NGPNPLSGTSAHPFFGDGMLHGVHLRDGKAQWYHNRYVQTPFISNPSVDILDASVMLD
                     MRASKANTHVVGHAGRILALEEGHFPYEVDADLNTVGPVDFDGVLTGSFTAHPKICPV
                     TGEMLAFGYSAFEPYLRYLRVSAEGKLVQTENITVGGPTMMHDFNITKNFVIFMDLPA
                     VFDMSLAMSGDMPIRWDDDYPARLGVMPRTGTDAQVTWYDINPCYVFHPMNSYEDGDT
                     IVLDVARFDHIWRESAMDFAPPQLWRWTIDTATGRVREEQIDDRPAEFPRVADSLIGL
                     RHRYGYMMAMATGDQGDPASQSGAILKYDRETGSRSEIELGRGHTCGEPVFAPAADAK
                     AEDDGYLMTYVYDAETDASRFVIMDAATMDDTPVASIELPRIPNGFHGSWIPSSVAG"
     gene            complement(8988..9740)
                     /locus_tag="AC16QF_01680"
     CDS             complement(8988..9740)
                     /locus_tag="AC16QF_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6447291.1"
                     /translation="MNKVKDRIALVTAAADGIGASTATLLAEEGAVVVVTDINGEGAV
                     KLADEFGGMGLEHDAGSEADWKRVINEVTDRYGRLDILVNNAGIGAGIGDIEHQTLDD
                     YRAVMRVCVDSVFIGSKLAIELMKRDGGAIVNVSSVHGIRAAAHEAAYSAGKGAVRLL
                     TKSIALYCAQCGYRIRANSVHPGYILTTMMKEWMERSGNSADIMAGLVQQHPIGFLGE
                     PRDVANGILYLASDDARFVTGSELVIDGGFML"
     gene            9868..10398
                     /locus_tag="AC16QF_01685"
     CDS             9868..10398
                     /locus_tag="AC16QF_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6447292.1"
                     /translation="MNQEDQMRPDAESRYHALPGGINGKEHAVNLLEKGSLRWKRFTG
                     GDKFDYPIDYSAALLNAREDGHVDLLYRWEPNCYCHFHRHTAETTSTVLEGELHVIDI
                     DLETGKEVGRRVRRAGDYAHKAPGDVHMERAGPEGALVLFSLYAPDGLLAESLAEDGT
                     VLGVSTMDRILKRRAS"
     gene            10446..11312
                     /locus_tag="AC16QF_01690"
     CDS             10446..11312
                     /locus_tag="AC16QF_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015016 - heparan sulfate
                     N-sulfotransferase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HIR6447293.1"
                     /translation="MRVYKDAVMDNARWEGFEPRPGDIFVCTPSKCGTTWMQMIVANL
                     LWPDGEFPGSIVNGICPWIEAKFTPAEKMHAMLRAQTHRRAMKSHTPADGIPWFDDAR
                     YITVVRDGRDAFMSWCNHVSRMMVTDMLNEKASEDGLPAINTFDGVDYRGYFHEWLER
                     NNFFDVVASYWAVRDRPNLLFVHFNDLKADLTGQMRRIAPFLDIELDEGQWPAVVKRC
                     TFEGMRKADKKVGDFEGFFEGGLEGFVYKGTNGRWRDVLNDDDIAAYRRKVAETLPPD
                     AARWVEFGARAT"
     gene            11417..11623
                     /locus_tag="AC16QF_01695"
     CDS             11417..11623
                     /locus_tag="AC16QF_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447294.1"
                     /translation="MSIRARGRLLIAALSGLLLPAAYAVTIDIAEVRDEMAFVKGSDA
                     GRKQQILWEGAPATTANGKGNFSF"
     gene            11801..12034
                     /locus_tag="AC16QF_01700"
     CDS             11801..12034
                     /locus_tag="AC16QF_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447295.1"
                     /translation="MGFVDDGLTIKVVSGGADTNLRYWTNELHELEIGHDLPNIIYDL
                     EVSNDYSAVVTGEGGWNGSAGASTTSPFLRKAT"
     gene            11977..12102
                     /locus_tag="AC16QF_01705"
     CDS             11977..12102
                     /locus_tag="AC16QF_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HIR6447296.1"
                     /translation="MERKRGRVNDVAFSPEGDLIVSGGADGTLKLWAYPDPPPPD"
     gene            12272..13273
                     /locus_tag="AC16QF_01710"
     CDS             12272..13273
                     /locus_tag="AC16QF_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012377.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HIR6447297.1"
                     /translation="MGWLVGCNFTPSTAGNQLEMWQSESFDPETIDRELGWASDLGMN
                     VVRVYLHDLLFEADGNAFLARIDEVLAIAHRHGIGMMPVLFDGVWHPKPKLGAQPNPV
                     PRLHNSIWVQSPGNAILQDRSQWPALKPYVTGVLSRFAADERIIAWDLFNEPDQIDQI
                     TIAGGSRDEKAAAATELLVCVFDWAREVDPSQPLTAGLWEYGPDHRPVDNPINAVMLE
                     RSDVITFHCYEPRAALEAVIQALSAHGRPLVCTEWLARTAGSSVDLLEVFAEHQVGAI
                     NWGLVDGRTQTRFPWRSWFEAVDDDEPWFHELLHADGSPYDPEETATFRRVTGNERA"
     gene            complement(13322..14032)
                     /locus_tag="AC16QF_01715"
     CDS             complement(13322..14032)
                     /locus_tag="AC16QF_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015016 - heparan sulfate
                     N-sulfotransferase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HIR6447298.1"
                     /translation="MDGGASAIALGSIHRSRHRYTIEEYLEMVSGQDHRRFLKTHSPL
                     DGLPYYPECHYLVVFRDPRDMFFSMLKHRDNFTDEELAFASIPSGEDAFRQWLDRGFD
                     PKNFDFQSLEWLCHFLQTYWPYRDLPNIHLFHYSDMKNDLPGHVRKVAGILEVELTEA
                     QLDEMTAAASFEAMRAKGDQFAPNAGLGAWKSEKDFFAVGTNAQWKGKLTPEDLAAFE
                     SRIRDFLSEEQIAWLVRG"
     gene            14149..15108
                     /locus_tag="AC16QF_01720"
     CDS             14149..15108
                     /locus_tag="AC16QF_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6447299.1"
                     /translation="MSFGFESTTDQVLAGVDLAGKRAIVTGASGGLGEETARALAAKG
                     AAVTITARDLAKGEAAAQKIRESTGNAAVDVRGLELADLASVRAFAAGYLADHDKLDL
                     LINNAGVMACPLTRTPAGWEMQFATNHVGHFLLTCLLAPALKAAAPSRVVNLSSAGHH
                     MSAVDLDDPFFNDREYDKWVSYGQSKTANILFSVGLEKRLAGAGVHAYAVHPGGIMTE
                     LGRHLTDEDIAAMMERNNISDPEAFQAGFKTVPQGAATSVWAATSPELEGRGGLYCED
                     CHVAGPGAGGIRDGGYAPWALDPDAAERLWALSEELVDQRFEL"
     gene            15208..15564
                     /locus_tag="AC16QF_01725"
     CDS             15208..15564
                     /locus_tag="AC16QF_01725"
                     /inference="COORDINATES: protein motif:HMM:NF018777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HIR6447300.1"
                     /translation="MIKVVMPMKRRPGMTIPEFREYYETKHRVIGEKYLKGYATKYMR
                     RFTNPTVDRDGELRDPEYDVFLEIWYPDQETMEACGRVLSAPDAQQEIREDEEKLFDT
                     RFMRSYTVDESVSDLD"
     gene            15808..17034
                     /locus_tag="AC16QF_01730"
                     /pseudo
     CDS             15808..17034
                     /locus_tag="AC16QF_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
     gene            17091..17234
                     /locus_tag="AC16QF_01735"
     CDS             17091..17234
                     /locus_tag="AC16QF_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447301.1"
                     /translation="MTDPHEEREPARTGSLEQTDMGESLRQLWWTIGATVVGLAVIAY
                     FTL"
     gene            complement(17224..17685)
                     /locus_tag="AC16QF_01740"
     CDS             complement(17224..17685)
                     /locus_tag="AC16QF_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447302.1"
                     /translation="MNWDALDFAVFGAMLLGAGIVYAFARRKTRNSAYRAAVGVALGS
                     AFLLVWINGAVGILGDEDDVANMMFFGVLGIGAVGAAVCRFEARGMMLALAATACAQL
                     AVAVVALAAGLGSTSPSWPQHVLVLTALFVVMWLGSAWLFRSAAREQSFTK"
BASE COUNT         3477 a         5739 c         5491 g         3197 t
ORIGIN      
        1 catggtgacg ccggtgttcg aaaccatggg ggtggcggcc accctcaacc tggccgcgaa
       61 cctgatctgc ctgcgcatcc tgtttccctt ccggaacgac gacctgaaca tgacgtccgt
      121 ctgggagtgc tcgcgcaacg acatcttcga cggccttgca gtgctgaccg ccaccgtggc
      181 cgtgtgggtt tccgactccg catggccgga cctcttgatc gcgatcgctc tgctgatcgt
      241 tttcctgcga tccgctgccc gcattctcag ccgttcctgg cgggagctgc acgccgcacc
      301 cacgccgggg ccggggaccg ggtaggaaaa cggcgaccgg atgcagcgtc aggcagatct
      361 atgctcgccg ggccagaatc tcatccacca gctcgacgcc gccgtggtcc agggctgcct
      421 cggggaagtt ccgaatgatc ttcctgaaga tggcctggcc gccctcggtg gagacgtcca
      481 gatcgcctaa cgccatcggc aacagccgca ggccgatgat gatgccgtgc cggtagtcga
      541 accaggcttc atcgaagctg taacccggga tccccgcgtc caccagccgc tggtggtaac
      601 gactaaccag gttacgttca tgctcgcgac gaaacgccgg cgtgtacccc gaggtgatca
      661 gataccccag atcctgggcc gttctgccgg gatacattga ctgccaatcg tagagaacga
      721 ccggatcgtc ggattcctca tcgaggaaca cgttagccag ccgtaaatcc atgtggcaga
      781 gagacagatt ggtttccggt tgcgcagcag cgatcgtccg catgttctcg ccgagccgaa
      841 cggccagacc ctcgaacccg tcgggcgccc aagacatgaa ctcgctgtcg acgaagtcct
      901 tgtagccctc ctggatcccc gagaaatagc ctacaaaggc ggggtgatcg ggccccatca
      961 gctcatcctt tgtcgcgagc caatccgcat tccaggaaga aatcgccatg tcagcggcag
     1021 catcgacgat cagctccagc tccgccaagg tagtcggctg ctcgtcggtc tgcaccggcg
     1081 cgcgcatgtg cgagcaatcc tcgaggagca gaacaccggt tcccagctcg gcatcgtaat
     1141 cgcagtgata ggctcccggt gagcggatgg ctacgagcgg agcgatttcc gagtaaaact
     1201 gcttttcgcg cgcgaagagc ccgaactcgc ggcttagttt cctcgctccc tcgaatgtcg
     1261 gcgccagctt gcctacgagc tggcggggca acccgctccc gggccgtgtc tccagctcca
     1321 gaaagcacgc gaggctcacg aagcccgagt ccgcttcgga ggtcctcaca tcgacatcga
     1381 ggacttcgct ctcaggcacg ccaagcacct cgcacagcca gctcgtggtg aagtcctctg
     1441 gagtttcagg tatgcctgcc atctcgctgg ttctcctctc tatcccgatt tctcatccca
     1501 ccgacgccac gaccttgcca tggcgtcggg aattaggaaa gtcgccccgg cgcagccact
     1561 tgccgtaaca ttcgtgccgg accgcgatga ccagagttta aggagacaac gatgaaaaca
     1621 atcaaccgaa cgttgggtcg atccctgctg gtgctgattc ccttccttgc cctgaacggc
     1681 atgttcgcct acgccgattc ccacgggccg gctctcccat cgggcgtgct cgaagttttt
     1741 cagtgcaact acaacagcgg caaggatcgg gacgacctga acgccctgcg ggacttctac
     1801 ctgaagcagg cgaaaaaggt gaactacacg ccgcctgacg cgtttctctg gacgatgacc
     1861 aatggcagct ggcccaccac ccttacgtgg gtcaacgtcc acgaagacct gagcgcctat
     1921 tccagatctt acgataccat tgcggcctcg accgacctgg ccgcggtcaa cgagcgcgcc
     1981 gagacggtaa tgacctgcgt gcccgccctt ggaagctacg cccacgcgtt catacgcgac
     2041 cccgacgccc aaggaccctg ggaggcgacg gtggcttcct acgcctgcga tctgcgcccc
     2101 gagcaggaca tgagcgacct ggaagatcta atggaacacg tggcaacggt caatcaatcc
     2161 atgggcgacg atgccatgga tgaactgctg cacttcattc ccatgacggg tctaaacaac
     2221 cagccggatg tgttggtatt cgctgcgagc cggcgcggcg cttcgtcctg gtcgagccat
     2281 ctcgcgctcc tcgagcaatc gggtgccggg cgggcagttc tcgcgcactt tgattccgtg
     2341 ctcgattgcc ggttgagcct ttgggacgtg gaacagatgg tggggggata accgcgggca
     2401 cgccgcccgg ctcagcccgg ggcgaaattg aatcgcaacg acacgccgaa cgcccgcggc
     2461 tcgctcacgg aaaaatcctg ctgcgaatgc tgacggtacc cacctaggat gtgcaagtcg
     2521 ttttcgatgt tgcgcaccca ggcgcgcgcc gaccagcgtc cgtcgccggt ctcgtagatc
     2581 aggctcaaat tgtgctgatc ccaggcgtcg atggtctccg cgctgcgatt gaaaatcgtc
     2641 aggtagctct tgctctgcca gtagtagtcc caccgggcag tgagcgcccc ggcgggcacg
     2701 tcccacgtgt agctgacacc caggtgcacc gtgtgctcgg gcgtgtcggg gacggagttc
     2761 cccgacaccg gcacgggtat cccgggtaac agctccacgc ccacggcaga gagaaatgcc
     2821 ccgtcgaacc aggccgggat gccgttggga tagatggttg cgggcgcttc atccggcccg
     2881 atggccgctc ctatggcaat cgcgtggttc acccagggca gcacgtcctc aacgcgggca
     2941 acgtagcggc cattgggaga tccggcgttg gtggcgaaag acagcagctc cacatattcg
     3001 gggtcgccgt tggtcagcca cagggcgtcg atgcggggtg gttcattctt gagctcggca
     3061 ttgagccagg agtagccaat ctccagctcg gctctcggcg cgaagggcgg tcgccacctg
     3121 gcctcgatct ccgcgccgta catgtccgcg tccacgttgg tgttctcgat gttgcgggta
     3181 aactgctccg cggccaggtt ggtgagctgc agcccctcat agtcgttgaa gaatgcggcc
     3241 acgttcagca ttaggctgtc gtctgccagc agcgacttga agccgagctc gaaagagttg
     3301 acgtcctcgc ggtcgtaggt cgggttggtg ctgaagaagc cgttcaggcc gccgggcttg
     3361 tagccccggg cgaaggaagc gtaggccagc atgtcgggcg tgatctgcca gtcgaggaca
     3421 gcgcgtccac tgacggcgtc ccactcgaaa tccgtcggca cgccggcaat gatcgcgccc
     3481 tcgttccagg cagtcagaat cgggatctgc gatgcgatca ggttggcggc cagaaggccg
     3541 ggaaacccgc cggtcgccag ggcggcatcc acctcctcgg tcagaccgta aaagtcgagc
     3601 agacccgagt cgtcggagat tccaaatatg taaagggccg cttggggacg cacccacacg
     3661 tcgcggccga aaacgccgcc aaggccggtg ttgatgtcga aaggggcacc gatgccgcga
     3721 gctctgtagc gtttctcatc gcggttgtag cgcgcggcca ccgtgagttt caggtggtcg
     3781 gtcagatcca cgtagatctc cgacaaggcc gagaagctct cgaactcgtt tttgtcttcg
     3841 ccgccggcta aaaccatcag acgatcgaga gcgttcagat taagcgcgaa cggattcgga
     3901 cccgttgccg gggcctgtgc cctgccaaac cgggtccaca tctcccagta gtcgttggtc
     3961 ccgccacccg agacagcgtg gtcgtccgaa aactgatagt tgccgccgat cagaaagttg
     4021 acgcgaccgt ccagatccga gcgcagtcgc agctccgcgg agtagaagtc acgccactcg
     4081 cgtcgttccc cgtaccagaa ctggcgcgtc aagttggggt ccagcacgca gccgccaagc
     4141 acgccaaatt gcgcttcgtt gacgttgcat tccggcccgt cgagcgggtt cttgccttca
     4201 ggcagggcgg agaccggata caggccgctc ggattttccg gaatcggcgc caactcatgg
     4261 ccgacgctcc aatcgttatc ctgcaacgtg cgcaatcgcc agtcctgata gctgccgatg
     4321 aagctcaacg tcgcccaatc gaaatcgtgg ctggccccga gctgccagag gttctgctcg
     4381 aactcgtaca cgggggcgaa gtcgatgtgc acgtccctga gattggtgat ctcggggcgc
     4441 gggtaggcaa acgtgaggcc ggtttcagcg tcgcgcgccc caaggggaat gacgcctcgc
     4501 aggccgttgg tgatcgaggt ctgaaagcgg gcctggttcg ggttgttcgg ctccaggccc
     4561 cactcgccgg gctcgcagcc agtgcgcgtc gtgggccgca gcgccgtttt gcaacgacgg
     4621 ttgtgggtca gcatgcggtc gtcgtcttca tcgaagcgct cgtacaggcc ccagatctcc
     4681 gtcgcctccg tgacgcgcca ggtggcgctc gcccggacgc tgtagagatc tcgtccgtcg
     4741 atttcgctcg ccaccccggg cacctggccg ccggccaggt tgtccgtgta gccatcccgg
     4801 tccagagaga gtcccgctag gcgaagcgcg agccggtcgg cgaccgcggc gttcagcgcg
     4861 ccgcgcagcc gcaggaggtc gtggttaccg gcctccacgt ccaggtaccc gcttgtaccg
     4921 tcaaagctcg gcctgttggt aatgaggttg atgacaccgc ccgtggcgtt gcggccgtac
     4981 agcgtgccct ggggtccccg cagcacttca atccgggcga gatcgtagat ctcgaacggc
     5041 ggctggccgg atggcaaggg cacctggttg acgtgcaggg acacgcccga ctcgctgtcc
     5101 cggcgggaca ccaacgaccc gacaccgcga atggtgacgt gggtgtcccc cacgttgttg
     5161 gtgttgtagc tcaggttggg aacgaacagg gaaagatcgc cgatgccaat gatctggcga
     5221 tcctcgatgc ccgtctcgtc aaaggcgttg agggcgatgg gcaccttctg ggcgctctcc
     5281 tccacccgtt gagccgtgac gatcacctct tcgatgacca agcgctcctg agcctgcagg
     5341 gcgcacggca tcatcaggac catcgcgctg atcgggattc ctcgggccat gccgcgtctc
     5401 cctcttctgc cgagctaact tataacgcca cgagagccgc ctgaccaccc atggccgtct
     5461 aggccgttcg ccgaccgcgg ggatcgacga tggcatcgga cattcgagcc gctcgcgccg
     5521 ggcgtgcgcc acgaaaatcg gcctcgcgga gcgctaacgc agaaactcga tgtagaattc
     5581 gacctcgccg acgggagcga cctcgtggag caccgtcggc tcgacaacgc ccatgatgtg
     5641 tggggagagc agaaactctg ccgggtctgg ctcgaggatc cgataaagga gctcacccgt
     5701 cacgacgacg atccttcccc aggtgccggg cttggtgctg tgcgccttca gcaatccggg
     5761 cggaatcgag tcctggtgaa aagtcgccgt acgcctgtag ggaagtacgt tctctggcaa
     5821 tgctcgcatg tggagagtaa acgccatccg tttaatcgcc gccacctcgc cgcaccaatc
     5881 gaggatgccc ggtgtcggag atcccgacga gttcctgcgc caggcgtaac cgcctagaag
     5941 ttaaccccgc gggtaaatgc gccgtcgacg cgcacggtgg tgccgttcac ccaactcgcg
     6001 gcggggctgg cgagaaaggc gacaacgcgt gcgacctctt cccccgtgcc catgcgtttg
     6061 gtgggatggt agtcacggtc gcgctcatac agggctggcg cgttgtcctt gatgccgttc
     6121 caaacgccgt tctcgatgaa gatcggcccc ggcgcgacgg tgttcgcgcg aatgcctttg
     6181 ggtccccact gctgggccaa ttgcgccatg tgacgaatcg acgcggcttt catggcaccg
     6241 taactcgggc tgatggggac gtcgtggtgc tcaatgccgg tgatcgacgc gatctgtacc
     6301 agcgctgcac gctcggattg ctcgagaaag ggcagcgcgc cttcgctcat gcccaccatg
     6361 ccgagcacat cgacgctgaa gttgttgatc caggattggg gtcccggacc cggttggccg
     6421 ctggccgtgg cgttgtgcac aacgatgtcg ataccgccga gggcgtccgc tgcgttctgc
     6481 atccaagcca ccgtggcgtc gtagtctgca acgtcgcaca cctggccaac gacggtgccc
     6541 agcgcggaca gctcctcgag tgccgcgtcc acgccttctt gccggcgcgc cgcgatgctc
     6601 accgccaccc cctcgaccat gagctgtttg gcaaccgcca ggccgatacc ttgcgtgcca
     6661 ccggagatga cggcttttgc cccattaagt ccgagatcca tgtgctccct cccaggttct
     6721 gtatcgccta atagtcgcag aaccggcatc acggtaacaa caggcgcccc gcctgcacgg
     6781 tgggcggcat cgccggaatc cggacgcgcc cgttggctcc ggcatgttta agaggatcgc
     6841 ttagtgacca ctgggtcccc gccggttgcc tccatggtgg ctttacccat cttgcggccg
     6901 tcgaaaccga tggccttggt cggcgtgaac ttgatgacac ggcggttttc ggtgttgttc
     6961 agctcaacaa agacgcgccg tgctcgctcg tctcctatcc gtaaggcttt tgccagctcc
     7021 ggcaggaacc aatcgacggt ggcttggtct gagaacactt cgcaggtgcc cttgtaggtg
     7081 aggctctgtc cgctgcgcag cggtgtgccc ttgcttgtta tcgagatcga cgcgcgggga
     7141 tcgcgctgca ccgccttcac gcgaacgcgt aaagacgaca cgctgagcca gaagcaatcc
     7201 tccgcgtaga catacgtcat gatcaccccg accggccagc cgtctttggt ggaccacatg
     7261 aacgtgcact cgttctgcgc ggcgatgagg gcaagctcgt cggccttgtc cagggtgtag
     7321 gtgctgacgt cgtcgtagtt gaattctttg gacatcttac tcgggtcttc cagcagcaat
     7381 cgcgtgtgag agcgtatcat gcgccgtcgt tccgatccgg tcaccgctca actccacttt
     7441 tagggggagc gatgtcagcg cagaggcgga tcccgggtgg gttcgcctgg acccctcccc
     7501 gtcggcgaac tactatgagg caaccaatca ataagggaaa aacagatcat ggcagccgtc
     7561 atcgaagaaa tcccctccca cctgcgcggc aacggccgac cgatcaccga ggagcgcacg
     7621 ctcacggatc tgaaggtgac cggagcaatt cctcccgagc tggacgggcg ctacgttcgc
     7681 aacgggccga atccgctcag cggtaccagc gcgcatccat tcttcggcga cggcatgctg
     7741 cacggcgtgc atctgcgcga cggaaaggct cagtggtatc acaaccgcta cgtccagacg
     7801 cccttcatct caaacccttc cgtggacatt ctcgatgcgt ccgtcatgct ggacatgcgg
     7861 gcgtcgaagg cgaacaccca cgtcgtgggc catgccggca ggatcctcgc cctcgaggaa
     7921 ggtcatttcc cctatgaggt ggacgcggat ctgaatacgg tgggacccgt cgatttcgac
     7981 ggcgtgctca ccggatcgtt taccgcacac ccgaagatct gcccggtcac cggcgagatg
     8041 ctggcgttcg gctactcggc cttcgagccg tacctgcgct atctgcgcgt gtccgctgaa
     8101 ggcaagctgg ttcagacaga gaacatcacc gtgggcggcc cgacgatgat gcacgacttc
     8161 aacatcacga agaatttcgt catcttcatg gatctgcccg ccgtcttcga catgagcctg
     8221 gccatgagcg gcgacatgcc gatccggtgg gacgacgact acccggcgcg gctgggcgtt
     8281 atgccgcgca ccggcaccga tgcccaggtg acgtggtacg acatcaatcc gtgctacgtc
     8341 ttccatccca tgaattccta cgaggacggc gacaccatcg tcctggacgt tgcccggttc
     8401 gatcacatct ggcgcgaatc ggcaatggac ttcgcgccgc cgcagctctg gcgctggacg
     8461 atcgacactg cgaccgggcg ggttcgcgag gagcagatcg acgaccgacc ggcagagttt
     8521 ccccgcgttg cggacagcct gatcggcctg cgacaccgat acgggtacat gatggcgatg
     8581 gccaccgggg accaggggga tccggcgagc cagtcgggcg cgatcctgaa gtacgaccgg
     8641 gagaccggca gccgctcgga gatcgagctc ggtcgcggcc acacctgcgg ggagcccgtt
     8701 ttcgcgccgg cagccgatgc gaaggccgag gacgacgggt atctgatgac ctatgtctac
     8761 gatgccgaaa cggacgccag ccgattcgtc atcatggacg cggccacaat ggacgacacc
     8821 ccggttgcgt cgatcgagct gccacgaatt cccaacggtt ttcacggcag ctggattcca
     8881 agctccgtgg ccggctgagg gttcatcggc accggaaacc accacaggcg ccggatacga
     8941 cgcaccggcg cttccacccg cgcttgcagc cgcgcttgca gccgcgctca gagcatgaat
     9001 ccgccgtcga tgacgagctc ggaaccggtg acgaaccgcg cgtcgtcgct ggccaggtaa
     9061 agaatcccgt tggcaacgtc gcggggttca ccgaggaagc cgatggggtg ttgctggacg
     9121 agccccgcca ttatgtccgc gctgtttccc gagcgctcca tccactcttt catcatcgtg
     9181 gtcaggatgt agcccgggtg caccgagttc gcccggatgc gatagccgca ctgggcacag
     9241 tagagggcga tcgatttggt cagcagcctg accgccccct tgcccgccga gtaggccgcc
     9301 tcgtgcgcgg ccgcccttat tccgtgaacc gacgacacgt tgacgatggc gccgccatcc
     9361 cgcttcatca gctcgatggc gagcttgctg ccgatgaaca cactgtcgac gcacacccgc
     9421 atcaccgcac ggtagtcgtc gagagtctga tgctcgatgt cgccgatgcc cgcaccgata
     9481 cccgcattgt tgacgagaat atcgagccga ccgtaccgat cggtcacttc attaatcacg
     9541 cgcttccagt cggcttccga gccggcatca tgttccaggc ccatgccgcc gaattcgtca
     9601 gcgagcttca cggctccctc accattgatg tccgtgacga ccaccacggc accttcctca
     9661 gcgagcaggg tggccgttga tgcgccgatg ccatcggctg cagcggtaac gagcgcgatc
     9721 cgatccttta ccttgttcat ggctggttga cctccccccg gaaccagaat gagcggactg
     9781 acatgaaggc tgattatact gccgccggcg cgggatagcc ggttgccgaa ggacccgccg
     9841 cccaagaggt gaaggagtag cggcaccatg aaccaagaag accagatgcg accggacgcg
     9901 gaaagcaggt accatgctct tcctggcggc atcaatggca aggagcatgc ggtgaacctt
     9961 ctcgagaaag gcagtttacg gtggaagcgc ttcaccggag gcgacaagtt cgactatccc
    10021 atcgactatt cggcagcgct gctcaacgcg cgggaagacg gccacgtgga cctgctctac
    10081 cgctgggagc ccaactgcta ctgccacttt caccgccata cggcggagac gacgtccacc
    10141 gtcctcgaag gcgaacttca cgtcatcgac atcgatctcg agaccggcaa ggaagtgggc
    10201 cgcagggtga ggcgtgcggg agactacgcc cacaaagccc ccggggacgt gcacatggag
    10261 cgcgccgggc cggagggtgc cctggtgctc ttcagcctgt atgccccgga tggcctgctg
    10321 gcggaatccc tcgccgaaga tggcacggtg cttggcgtct cgaccatgga tcgcatcctc
    10381 aagaggcgag catcgtaacc gccccggatg cccgtacccc cgaatcccga aggaaggtca
    10441 ggtcgatgcg tgtctacaaa gatgccgtga tggacaacgc ccgctgggaa ggcttcgagc
    10501 cccgtcccgg cgacatcttc gtgtgcacgc cctccaagtg cggcacgacg tggatgcaga
    10561 tgatcgtcgc caacctgctc tggccggacg gcgagtttcc gggctcgatc gtcaacggca
    10621 tatgcccctg gatcgaggcc aaattcacgc cggcggaaaa gatgcacgcc atgctgcgtg
    10681 cccagactca ccgccgtgcc atgaagagcc atacccctgc cgacggcata ccctggttcg
    10741 acgacgcccg gtacatcacc gtggttcgcg acggccggga cgcattcatg tcctggtgca
    10801 accacgtcag ccgcatgatg gtcaccgaca tgctcaacga gaaggcttcc gaggacggcc
    10861 tgccagcgat caacaccttc gacggcgtcg actaccgcgg ctacttccac gagtggctgg
    10921 agcggaacaa cttcttcgac gtggtcgcaa gctattgggc cgtccgggac cggccaaacc
    10981 ttctcttcgt gcacttcaac gatctgaagg cagacctgac ggggcagatg cggcgcatcg
    11041 cgccatttct cgacatcgag ctcgacgagg gccaatggcc cgcggtggtg aagcgctgca
    11101 cgttcgaggg catgcgcaaa gcggacaaga aggtcgggga cttcgagggg ttcttcgaag
    11161 gcgggctcga aggattcgtc tacaaaggca cgaacggacg ctggcgcgat gtgctgaacg
    11221 acgacgacat tgccgcctac cgccgcaagg tcgccgaaac cctcccaccg gacgcggctc
    11281 gatgggtcga gttcggcgcc cgagccacct gatcccgccg agtactcgat cgcttcgccc
    11341 ggctaacaat cgcgccatat tcgccagcga actgaccggg tatcgtgaga tgggcaatcg
    11401 attgagcgca acgagtatga gcatccgggc acgcggccgt cttctcatcg cagcgttgag
    11461 tgggttgctc cttcccgccg cgtacgcggt caccatcgac atagcggagg tccgggacga
    11521 aatggccttc gtcaaaggca gcgatgcggg ccgaaagcag caaatactgt gggaaggcgc
    11581 acccgccaca accgcgaacg gcaaggggaa tttcagcttt tagggccccc tgcccaatcc
    11641 ctgcgtaccg gaagattgca gcgggacgct gactgccggc gaccatgtag agccggtaac
    11701 actgaccttt cccccggcgg agccgcctgt ccagccggac gaggcgctcg ttcaggtcgc
    11761 cgcccaagcc gacgcgcaca acggcgaagc gctccggacg gtgggcttcg tggacgacgg
    11821 tttaaccatc aaggtggtct ccgggggcgc cgataccaac ctgcgctact ggaccaatga
    11881 gttgcatgaa ctggaaatcg gccacgatct gcccaacatc atctacgact tggaagtctc
    11941 taacgactac tccgctgtcg taacaggcga aggcgggtgg aacggaagcg cgggcgcgtc
    12001 aacgacgtcg ccttttctcc ggaaggcgac ctgatcgtct ccggtggcgc cgacgggacg
    12061 ctcaagctgt gggcttatcc cgaccctccc cctccggatt agggttcttt tcttccctga
    12121 tccgctattc ttgcctggca gtccacctgg aacgcgtcgg gcgacgcaca ctgcgcaatg
    12181 gccgatggtt ttcgtctgaa cggagtgacc ggctaaggaa atgacagcac aaagatggtc
    12241 gaccgaacgc gccgcgcgtt ggcaaaccga cgtgggctgg ctcgtgggct gcaacttcac
    12301 gccctcgacg gcgggcaacc agctggagat gtggcagagc gaatccttcg atcccgagac
    12361 catcgaccgg gaacttggat gggcgtcgga cctgggcatg aacgtggtcc gcgtctacct
    12421 gcacgatctt ctgttcgagg ccgatgggaa cgctttcctc gcgcgcatcg acgaagtgct
    12481 ggccatcgct caccgccacg gcatcggcat gatgcccgtg ctcttcgacg gcgtctggca
    12541 cccgaagccg aagctcggcg ctcagccgaa ccccgtgccg cgtcttcaca actcgatctg
    12601 ggttcagagc cctggcaacg ccatcctgca ggaccgatcg cagtggccgg ccctcaaacc
    12661 gtacgtaacc ggggtgcttt cgcgattcgc agccgacgag cgcatcatcg cgtgggacct
    12721 gttcaacgag cccgatcaga tcgaccaaat aaccatcgcg ggcggatcac gcgacgagaa
    12781 agcggctgcc gccaccgagc tgctggtctg cgtcttcgac tgggctcgcg aggtcgaccc
    12841 gtcgcaaccg ctgacggccg gcctctggga gtacggaccc gaccaccgcc ccgtcgacaa
    12901 ccccatcaac gccgtcatgc tggagcgcag cgacgtcatc acgttccatt gctacgagcc
    12961 gcgcgctgcg ctcgaagcgg tcatccaggc gttgtctgcc catggtcgcc cgctggtgtg
    13021 caccgaatgg ctggcacgga ccgcgggatc cagcgtagat ctgctcgagg tgttcgcaga
    13081 acaccaggtg ggcgcgatca actggggcct cgtcgacgga aggacgcaga cacgctttcc
    13141 ctggcggtcg tggttcgagg cagtggatga cgacgagccc tggttccacg agcttcttca
    13201 cgcggacggc tccccgtacg atcccgaaga gaccgcaacg ttccggcgcg tcaccggaaa
    13261 tgagcgtgcc tgaccgagcc cctgagcagc tcccgtcagg aggatccgag aaacctggca
    13321 gctaacctcg aaccagccac gcgatctgct cttcagagag aaaatctctg atcctgctct
    13381 caaacgccgc aagatcctct ggcgtgagct ttcccttcca ctgcgcgttc gtgccgaccg
    13441 caaaaaaatc cttctcgctc ttccacgcac ccagacctgc attcggtgcg aactgatctc
    13501 ccttcgcgcg catggcttcg aacgaggcgg ccgcggtcat ctcgtcgagc tgggcttccg
    13561 tcagctctac ttcgaggata ccggcaactt tgcggacgtg gcccggcagg tcgttcttca
    13621 tgtccgagta atggaaaaga tggatgttcg gaagatccct gtagggccag taggtctgca
    13681 gaaagtggca cagccactca agcgactgaa agtcgaagtt cttcgggtcg aagccacgat
    13741 ccagccattg ccgaaaagcg tcctcaccgc tcggtatgga ggcaaatgca agctcctcgt
    13801 cggtgaagtt gtcgcggtgc ttgagcatgg aaaagaacat gtctcgggga tctcgaaaca
    13861 ccaccagata atgacactcc ggatagtagg gcagcccgtc cagcggcgag tgcgtcttga
    13921 ggaaacgccg gtgatcctgc cccgagacca tctccaggta ctcctcgata gtgtatcggt
    13981 gacgcgaccg atgtatcgag ccgagcgcga tggcagaggc tcccccatcg atttctcctc
    14041 ctctgactga gcgccctcac ggagaataac gggacattcg ggcgaaagac acgtatccga
    14101 ccatattggc gttatcatcc ggcgtatcaa gaaattgagg gacacgaaat gagctttggc
    14161 tttgaaagca ccacggacca agtgctggcg ggcgtcgacc ttgccggcaa acgcgccatc
    14221 gtgacgggcg cttctggcgg cctgggagag gaaaccgctc gggccctggc cgcaaaaggc
    14281 gcggccgtca cgatcacggc ccgggacctg gccaagggcg aggccgccgc gcagaaaatc
    14341 cgcgagagca ccggtaacgc agccgtagac gtccgcgggc tggagctcgc cgatctcgcc
    14401 agcgtgcggg ctttcgccgc ggggtatctg gccgaccacg acaagctgga tctgctgatc
    14461 aacaacgccg gtgtcatggc gtgcccgctc acgcgtaccc cagcgggatg ggagatgcag
    14521 tttgccacca atcacgtcgg ccacttcctg ctgacctgcc tgcttgcccc ggcgctgaag
    14581 gcggcggcac cgtcgcgcgt cgtcaacctg agctccgccg ggcatcacat gagcgcggtg
    14641 gatctggacg acccgttctt caacgaccgt gagtacgaca agtgggtgtc ctacgggcag
    14701 tcgaagaccg cgaacatcct gttctctgtc gggctggaaa agcgcctggc cggcgcgggc
    14761 gttcacgcct acgcggtaca tcccggcggc atcatgaccg agctcggccg gcatctgacg
    14821 gacgaggaca tcgccgccat gatggagcgc aacaacataa gcgatcccga agcctttcag
    14881 gccggcttca agacggtccc ccagggagcg gcgaccagcg tgtgggccgc cacctcaccc
    14941 gagctggaag gccgcggcgg cctctactgc gaggactgcc acgtggccgg acccggcgcc
    15001 ggtggcatcc gcgacggcgg ttacgcgccc tgggcactcg atcccgacgc ggccgaacgg
    15061 ctgtgggcct tgtctgagga actggtggat cagcggttcg agctctgaag gatcagccgc
    15121 gtcggctctc ggaaagcggc acaacctctg tgccgctgcg ctgtgcatgt gtagtcttta
    15181 ccacctgaca agttgcggag ggagccaatg atcaaggtag tgatgcccat gaaacgccgg
    15241 ccgggaatga cgattccgga gtttcgcgaa tactacgaga cgaaacatcg cgtcatcggc
    15301 gaaaaatacc tcaagggata cgccaccaaa tatatgcggc gctttacgaa tccaaccgtg
    15361 gaccgggacg gcgagctgcg cgaccccgag tacgacgtgt ttctggaaat ctggtatccg
    15421 gaccaggaaa ccatggaagc gtgcgggagg gtcttgagcg cgccggacgc tcagcaggag
    15481 attcgcgagg acgaggagaa gctgttcgac acgcgcttca tgcgttccta caccgtcgac
    15541 gaaagcgtgt ccgatctgga ctgagcgcgc ggcgggtagc aagctgcagc tttctccggc
    15601 cgcgatcggc acgagccgtc ccgcggtttt gacatttccg tcccccgtca gtctaatttc
    15661 cgaagcgcaa acgaggtcct acctatgagc gacattctca gacatccgtt ggccatggac
    15721 gggaaagatc cggctaatct tcgtggagac cccatctccc cggagcgcta ctacagcaag
    15781 gagtggatgg cgaaagagtg ggagcttatg tggaccaaga tctggcacat cgccggtcgc
    15841 gaacagcagc ttcaggaccc gggcgactat atcgtgcaca acttcatgca cgagtctgtg
    15901 atcatcgcca agcagaagga cgggtccctg aaaggctttt acaacgcctg tggccaccgt
    15961 gcccagcggt tgctctggag cgagcacggc tcccaggaac aaatgcattg cccctaccac
    16021 ggctgggtct ggggcatgga cggctctctg ctggatagct gtgaccggga cgacttcccg
    16081 gaagacccgg tgggtaagat caggctcatc gaagtgcgcg tggacacctg ggcagggctg
    16141 gtctggtaca cgatggacga cgaggcaccg gacctgatga cgtacctcgc ccccaacccc
    16201 gagctctaca agggccacca gttcgagaaa acggtgcgcg tcaactggtg gcgcgtcgcg
    16261 ctcaatacca actggaagtt ctggtccgat aacttcaacg agtcgtatca cacccgcacg
    16321 gtccaccctc aggtaccccc gatcatcgac caggaccatt tcacctcgcg ctacgaaatg
    16381 ttccccatgg gccacaatcg catcgtccag atgggccggc cctctctgcg ggatcgtcta
    16441 cccaaaggcg cgccggaccc gttcgaagcg acgctgcgcg cgtgggacat cgatcccgag
    16501 cagtacccgg atttcgagac caaggcgctc cagggctgga aggacctcaa ggcagcgaag
    16561 cgaaggctct ggagggagag aggctttctc cactacgaga acctgaacga cgaggatctg
    16621 acggaaagcc cctttggcgt gctgtttccc aacgtcgcca tcgcgcccgg ttccgattct
    16681 ttcctggtat ggcgctggga accgcatccg acggatcccg agaagtgcta cttcgatcaa
    16741 tggaccatgg cttagcccat cgagggtcag gaagaattcg tcaaccgcac ggccgagaac
    16801 ccgctcgagc tgaaagaggc agagctggat ttctgtgact atgacgacgg gcgcggtgtt
    16861 caggattact ccgatcaggt ggtgtatcag gacatgcatc tgaccgccgg tcagaccagc
    16921 ggttggcgct ccagggggta tcaggaaccc tatatggcgg cccaggaaac ccgagtccgg
    16981 cgcttccacg aggtgctaca cgactacctg gcggggaatc cgccggggcg ctgaatgttt
    17041 ctcgcatcga tccgagctca ggcggcaaga cgcgcgacag gaaacaccag atgactgacc
    17101 cacacgaaga aagggaacca gccagaaccg gaagcctcga gcagaccgac atgggagagt
    17161 ctcttcggca gctgtggtgg accatcggcg caaccgtcgt cgggctggcc gtcattgcat
    17221 actttacttt gtgaacgact gctcacgcgc ggcgcttcga aagagccacg cagagcccag
    17281 ccacataacg acgaataacg cggtgaggac aagcacgtgt tgcggccagc tgggcgacgt
    17341 ggaacccagg ccggcggcga gggcgaccac cgccacggcc agctgagcgc acgccgtcgc
    17401 cgccaacgcg agcatcattc cccgcgcctc gaagcggcag acggctgcgc caacggcgcc
    17461 aatgccaagc acaccgaaaa acatcatgtt ggcgacgtca tcctcatcgc ccaggatgcc
    17521 gacggcaccg ttgatccaga ccaggaggaa cgcagagcca agggcaacgc caacggcggc
    17581 ccgatatgcc gagttccgcg tcttccgacg cgcgaacgcg tagacgatcc ccgcacccag
    17641 caacatcgcc ccgaaaacgg cgaagtccaa ggcatcccaa ttcacctgct cctccttcgc
    17701 ttcacggccg aactcgctgg gggcaactca gagcacggcg aacagcttga gctcgctgcc
    17761 cacctctttg gcgagataca acgcgcggcc ctcatgaatc agctggtcgt cggccccgta
    17821 ctgctcatat cgcatagtga gcgtatgcac gccaggcctg aggcgcgcgt agtccagacc
    17881 caggacctcc gtgcgcgcag cctt
//
LOCUS       DBNHLM010000037         5771 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_543421, whole genome shotgun sequence.
ACCESSION   DBNHLM010000037 DBNHLM010000000
VERSION     DBNHLM010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_543421"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /gene="ppsA"
                     /locus_tag="AC16QF_01745"
     CDS             <1..1403
                     /gene="ppsA"
                     /locus_tag="AC16QF_01745"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017845789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HIR6447303.1"
                     /translation="VASRANPNRLVHYRLSGSAPELARGRAVGSAVATGTARVIRDPR
                     EIRDFVEGDVLVADTTAPDWGAVIKKASAIVTNRGGRTCHAAIVARELGIPAVVGSGT
                     ATEQIPSGDVVTVSCAEGEVGRVLAGTVAFETETMDLESLPRTRTQMMLNVGNPDVAF
                     KMAMLPNDGVGLARLEFIITEYVRAHPMALLHPERIQDPGIERELRELCRGYADPAEF
                     FVARLAEGVGTIAAAFYPKPVVVRLSDFKSNEYADLLGGRAFEPVEENPMLGFRGASR
                     YAHPAYEAGFALECAALKRVRETMGLHNVRVMVPFCRRVEEAARVLATMAAHGLARGE
                     NGLEVYMMCEIPNNVLLIDEFSAYFDGFSIGSNDLTQLTLGVDRDSEMVAFEFDERDP
                     GVLRMLELAVAGAKRNGKHSGICGQAPSDYPEIAEFLVRRGIDSLSLNPDSVVAATEL
                     VAQVEAKIAAEGADGA"
     gene            complement(1465..3123)
                     /locus_tag="AC16QF_01750"
     CDS             complement(1465..3123)
                     /locus_tag="AC16QF_01750"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6447304.1"
                     /translation="MAHDLVIRGGTIADGSGADPVPGDVAVNGTTITEVGNVEGAGRR
                     EIDARGLLVTPGFVDTHTHLDAQIGWDPMLTPVSWHGVTTALLGNCGVTFAPCHASDR
                     DFLAGMMETVEDIPKDAILGGLPWSWEDYGGYLDALQDLNPAINVAGLVGHCAVRYYV
                     MGERSIDDNSTAEERQRMAEVVADAVRAGAVGFSTSRFLGHYIPDGRHVPGTHADHDE
                     LVQIAAEVGKLGGLMQNVLNLGGDFEGEISLLAAEARASGQRVLFSNTAGKTQAWGDK
                     LNARISALRAEGLDINGIAIPRGSGFVTGLQAMLVWQGPTWGQLAKQDLSGRLQAIAD
                     PEFRARLIAEAEENPLRFDTRQMFWLGDGERPDYTCDGRNSLQALADANDELPVETFL
                     RLSRETAGKALFTIRFFNQNVDALAELISTDWCLPGLGDAGAHVSQIMDSGWATFVLS
                     HWARDAGLYTVGEAVRRITSAPARIIGLSDRGTVAPGMRADLNVIDFDGLHEQMPEMV
                     HDFPGGAPRFIQRASGYRATLCNGQVILENDEHTGARSGEVIRR"
     gene            complement(3202..4071)
                     /locus_tag="AC16QF_01755"
     CDS             complement(3202..4071)
                     /locus_tag="AC16QF_01755"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6447305.1"
                     /translation="MQLEGQVAVVTGGASGMGRDTVLRFLAEGARVVIADFNARTGQA
                     VLEEARAAGFGESTRFVRTDVAREADVEAMCGLAVEAFGRLDVVFNNAGVGGAIGPLT
                     ETTVESWDYTMDVLAKGVFLGIKHAARIMREQGEGGAIINTASIAGLSGDAGPMIYSA
                     AKAAVISLTKSAAVELAPDRIRVNAICPGFIATPLADGGRRDETRERFATSQPWPEHG
                     DGTHIAGAAVFLAGADSRFVTGEALIVDGGLTAAGPELSKRFPRASGRHARVSGVTKG
                     STGEAPELKRLEE"
     gene            4179..4598
                     /locus_tag="AC16QF_01760"
     CDS             4179..4598
                     /locus_tag="AC16QF_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR6447306.1"
                     /translation="MTYDPDNVFAKIIRGQAPAHRVYEDAWTLAFMDLMPQTDGHTLV
                     IPKSEAVSLLDVDSEALARTIQTTQKIAAAVKAAFGAHGVMVAQLSGAAAGQTVFHLH
                     FHVLPRLHGADFEIHAREVAPAEVLAEHAERIRRALG"
     gene            complement(4595..>5771)
                     /locus_tag="AC16QF_01765"
     CDS             complement(4595..>5771)
                     /locus_tag="AC16QF_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6447307.1"
                     /translation="TALHLSAQGHCVVIVDNLSRRNIDNELEVQSLTPIRPLGERIGT
                     WYELTGREIQFVRMDVATEYDRLLELIRSHRPDAIVHFAEQRAAPYSMKSSWHKRYTV
                     NNNLNATNNVLAAIVDSRQDIHLVHLGTMGVYGYSSVGVKLPEGYLNVKMTNDDGEEV
                     EQEILYPANPGSIYHMTKTQDQLFFAFYNKNDQVRVTDLHQGIVWGTQTSETRLDERL
                     INRFDYDGDYGTVLNRFLMQAAIDYPLTVHGSGQQTRAFIHLQDTVRCVELAISNPPA
                     AGERVKILNQLTETHRLIDLARLVSEMTGVPIEHVPNPRREADANDLAASNEGLVGLG
                     LQPTTLKGGLLEEVTDIARRYAERCDRTRIPCMSHWTTDIAGDIEVIVPSGEASTAG"
BASE COUNT          913 a         1888 c         1942 g         1028 t
ORIGIN      
        1 ccgtggcttc gcgcgcgaac ccgaaccggc ttgtgcatta ccggttgtcg ggctctgcgc
       61 cggagctggc gcgcgggcgg gccgttggca gcgcggtggc aaccggcacc gcccgcgtga
      121 tccgggatcc acgggagata cgcgactttg tcgagggcga cgtgctcgtc gcggacacca
      181 ccgcgccgga ttggggagcg gtgatcaaga aagcctcggc catcgtcacc aatcgtggcg
      241 ggcgaacgtg tcatgcggcc atcgttgccc gggagctggg catccccgcc gtggtggggt
      301 cgggaacggc taccgagcag atcccatccg gcgacgtggt gaccgtttcc tgcgcggaag
      361 gcgaggtggg acgtgttctg gcaggtacgg tcgccttcga gaccgagacc atggacctgg
      421 aatccctgcc caggacgaga acccagatga tgctcaacgt cggaaatccc gacgtcgcct
      481 tcaagatggc catgctgccg aacgacggcg tcggtcttgc ccggctggag ttcatcatca
      541 ccgagtacgt ccgcgcgcac cccatggcgc tgctgcaccc ggaacgcatc caagatccgg
      601 gtatcgagcg ggagctcaga gagctgtgcc gcggttacgc cgaccccgcc gagtttttcg
      661 tggctcgcct ggcggagggg gtcggaacca tcgccgccgc tttctatccg aaaccggtcg
      721 tggtgcgcct gtccgacttc aagagcaacg agtacgccga tctgctgggt ggccgggcct
      781 tcgaacccgt cgaggaaaac ccgatgctgg gtttccgtgg cgcttcccgg tatgcccatc
      841 ccgcctacga ggcggggttt gcgctggagt gcgctgcgct gaaacgggtc cgcgagacca
      901 tgggtcttca caacgtccgc gtgatggtgc cgttctgccg gcgcgtcgag gaggcagcgc
      961 gggtgctcgc caccatggcc gcccacggcc tggcacgggg cgaaaacggt ctggaggtct
     1021 acatgatgtg cgagatccca aacaacgtcc tgctgatcga cgagttctcc gcgtatttcg
     1081 acggcttttc catcggcagc aacgacctga ctcagcttac ccttggcgtc gaccgggact
     1141 cggagatggt ggctttcgag ttcgacgagc gggatcccgg tgtgctcagg atgctggaac
     1201 tcgcggttgc cggtgccaag cgtaatggca agcactcggg catctgcggg caggcgccct
     1261 cagactatcc cgagattgcc gagttcctcg tgcgccgggg catcgattcg ctgagcctga
     1321 atcccgattc ggtggtagcg gccaccgagc tggtcgccca ggtggaggcg aagattgccg
     1381 ccgagggggc cgacggcgcc tgagcgcgtc gaccccggag cgggcgatgc cctgggcggc
     1441 gagcgtaaag gttgcccgcc cggctcagcg gcggatcacc tcgccgctgc gcgcgccggt
     1501 atgctcgtcg ttctccagga tcacctgacc gttgcagagc gttgcgcggt atccgctggc
     1561 gcgctggatg aaccgcggcg cgcccccggg gaagtcgtgc accatctccg gcatctgctc
     1621 gtgaagaccg tcgaaatcga tgacgttgag atcggcccgc atgccggggg cgacggtgcc
     1681 gcggtcgctc agcccgatga ttctcgccgg cgcggaagtg atccggcgca cggcttcacc
     1741 caccgtgtac aggccagcgt cacgcgccca gtgggagagc acgaaggtag cccagccgga
     1801 gtccatgatc tggctcacgt gggcgcccgc gtcgcccagg ccgggcaggc accagtccgt
     1861 tgagatcagc tcggccagcg cgtcgacgtt ctgattgaag aagcggatgg tgaaaagggc
     1921 cttgcccgca gtctcccggc tcaggcgcag gaacgtctcc acgggcagct cgtcgttggc
     1981 gtccgccagg gcctgcaggc tgttgcgacc gtcacaggtg tagtcgggac gttctccgtc
     2041 gcccagccag aacatctggc gcgtgtcgaa tcgcaagggg ttttcctcgg cttccgcgat
     2101 cagccgggcg cgaaactcgg ggtcggcgat ggcctgcagt cggcctgaga ggtcctgctt
     2161 ggcgagttgc ccccaggtgg gcccctgcca gacgagcatg gcctgaagcc cggtaacgaa
     2221 gccggacccg cggggtatgg cgatgccgtt gatgtccagc ccctcggcgc gcagcgcgct
     2281 gatgcgggca ttgagcttgt cgccccaggc ctgggtcttg cccgccgtgt tgctgaacag
     2341 cacccgttgc ccgctcgccc gcgcctcggc ggccagcagg gaaatctctc cctcgaaatc
     2401 accgccgagg ttcagcacgt tctgcatgag cccgcccagc ttgccgacct ctgccgcaat
     2461 ctgcacgagc tcgtcgtggt cggcgtgggt gcccgggacg tggcggccgt ccgggatgta
     2521 gtgcccgagg aaccgggacg tggagaagcc gacggctccc gcccgcaccg cgtcggccac
     2581 cacttcggcc atccgctgcc tctcctcggc ggtggagtta tcgtcgatgg atctctcgcc
     2641 catcacgtag tagcgcacgg cgcagtggcc gacgaggccg gccacgttga tggccgggtt
     2701 cagatcctgc agggcgtcca gataaccgcc gtaatcttcc cacgaccagg gcagcccgcc
     2761 gaggatggca tccttgggga tgtcttccac ggtctccatc atcccggcca ggaagtcgcg
     2821 gtccgatgcg tggcagggag cgaaggtgac gccgcagttg ccgagaaggg cggtggtgac
     2881 gccgtgccag cttaccggag tgagcatggg atcccagccg atctgggcat ccaggtgagt
     2941 gtgggtgtct acgaaaccgg gcgtcacgag aagccccctg gcgtcgatct cgcggcggcc
     3001 ggcgccctcc acgttgccaa cctcggtgat cgttgtgccg ttcaccgcca cgtcaccggg
     3061 gaccgggtcc gctccgctgc cgtcggctat ggtgccgccg cgtattacca ggtcgtgcgc
     3121 catgggttgc ctccgttatc gtcaaacgca aaccatcgat gctagatcaa ccggcttgct
     3181 gcgggcaagg atggccgagc tctactcctc cagacgcttg agctccggtg cttctcccgt
     3241 gctgcccttg gtgacgccgc tcacccgggc gtggcggccg gaggcccttg gaaagcgctt
     3301 ggagagctcg ggtcccgcgg ctgtcagccc gccatcgacg atgagcgctt caccggtgac
     3361 gaatcgcgaa tcagccccgg ccaggaacac ggccgccccg gcaatgtggg tgccatcgcc
     3421 gtgctcgggc cagggctgtg acgtggcaaa gcgctccctg gtttcatccc gccggccgcc
     3481 gtcggccagc ggcgtggcga tgaagcccgg gcagatggcg ttcacgcgaa tccggtccgg
     3541 tgccagctcc accgcggcgg acttggtgag gctgatgacg gcggccttgg ccgcagagta
     3601 gatcatcggg ccggcatcgc cgctcaaccc tgcaatggac gcggtgttga tgatggcacc
     3661 gccttcgccc tgctcacgca tgatccgtgc ggcgtgcttg atccccagga agacgccttt
     3721 ggcaagcacg tccatggtgt aatcccatga ctctaccgtg gtctcggtca acgggccgat
     3781 cgcgccgccg accccggcgt tgttgaacac gacgtcgagc cggccgaagg cttccacagc
     3841 cagaccgcac atggcctcca cgtccgcctc ccgcgccacg tcggtgcgaa cgaatcgcgt
     3901 cgactcgccg aatccggcgg cgcgcgcctc ttcgagaacg gcctggcccg tccgggcgtt
     3961 gaagtccgcg atgaccacgc gtgcgccctc ggcaagaaaa cgcagtaccg tgtcgcggcc
     4021 catgccgctg gcgccgcccg tcaccacggc gacctggcct tctagctgca tcgcattccc
     4081 cccgcgcaaa acgacagatt accaccggtg cccgatgacg cgccttttcg aatatgctcg
     4141 acgtctgaac cggagaccgg agtaacaccg gagcaacgat gacttacgat cccgacaatg
     4201 tcttcgccaa gatcatccgc ggccaggcgc ccgcccaccg ggtttacgag gacgcctgga
     4261 cgctggcgtt catggacctc atgccgcaga cggacggcca caccctcgtc attcccaaga
     4321 gcgaggcggt gagcctgctg gatgtggact ccgaggcgct tgcccggacg atccagacga
     4381 cccagaagat cgccgcggcg gtgaaggcgg catttggcgc ccatggggtg atggtggctc
     4441 agctgagcgg ggcggccgcc gggcagacgg tctttcacct tcactttcac gtgctgccca
     4501 ggctgcatgg cgccgacttc gaaattcacg ccagggaggt ggcgcctgcc gaggtgctcg
     4561 ccgagcacgc agagcgtatt cgccgcgcgc tcggctagcc ggcggtcgaa gcctcgccgc
     4621 tggggacgat cacctcgatg tcccccgcaa tgtccgtcgt ccagtgggac atgcagggga
     4681 tgcgcgtccg gtcgcagcgt tcggcatagc gccgggcgat gtcggtcacc tcttcgagca
     4741 ggccgccttt cagcgtcgtc ggctgcaggc cgagccccac cagcccttcg ttgctcgcgg
     4801 ccagatcgtt tgcgtcggcc tcccgacgcg gattcggcac gtgctcgatg ggcacgcccg
     4861 tcatctccga gaccagccgg gcgagatcga tcagccggtg ggtttccgtc agctggttca
     4921 gaatcttcac ccgctcgccc gcggccggcg ggttggatat ggcgagctcg acgcagcgca
     4981 ccgtgtcctg aaggtggatg aaggctcgcg tctgctgccc gctgccgtga acggtgaggg
     5041 ggtagtcgat ggcggcctgc atcaggaaac ggttgaggac cgtgccgtaa tcgccgtcgt
     5101 agtcaaagcg gttgatcagc cgctcgtcca ggcgggtctc gctcgtctgg gtaccccaga
     5161 caatgccttg gtgcaggtcg gtgacgcgca cctggtcgtt cttgttgtag aaggcaaaga
     5221 acagctgatc ctgggttttg gtcatgtggt agatgctgcc cgggttcgcc ggatagagga
     5281 tctcctgctc cacctcctcg ccgtcgtcgt tggtcatctt cacgttcaga tagccttccg
     5341 gcagcttcac gcccacgctg gagtagccgt agacgcccat ggtgcccagg tgcaccaggt
     5401 ggatgtcctg gcggctgtcg acgatggcgg ccagaacgtt gttggtggcg ttgaggttgt
     5461 tgttgaccgt gtagcgtttg tgccaggaag acttcatgga gtacggtgcg gcacgctgct
     5521 cggcaaagtg cacgatggcg tccgggcgat ggctgcggat cagctccagc aaccggtcgt
     5581 actccgtcgc cacatccata cgcacgaact ggatttcccg tcccgtcagc tcgtaccagg
     5641 tgccgatgcg ctcgcccagc gggcggattg gggtaaggct ctgaacttcg agctcgttgt
     5701 cgatgttacg cctggagagg ttgtcgacga tgaccacgca atgaccctgt gccgacagat
     5761 gcagggcggt a
//
LOCUS       DBNHLM010000038        12976 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1262177, whole genome shotgun sequence.
ACCESSION   DBNHLM010000038 DBNHLM010000000
VERSION     DBNHLM010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12976)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12976)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12976
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1262177"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="AC16QF_01770"
     CDS             complement(<1..490)
                     /locus_tag="AC16QF_01770"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR6447308.1"
                     /translation="MATTYLKRAEPAAAPDEEGIAVRVAEMLAKIRSEGEPAVAGYAR
                     DLDGFDGDFVLSREARDRLIAQLPSETRDDITFAWRQIKRFAEAQRASLHEFEIQTEP
                     GVTLGQRIVPVQVAGCYVPGGRYAHAASALMSVTTAKVAGVPTVVACSPPRGGAIHPA
                     VAF"
     gene            complement(490..768)
                     /locus_tag="AC16QF_01775"
     CDS             complement(490..768)
                     /locus_tag="AC16QF_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447309.1"
                     /translation="MANGVIRIPALWLIASAVQAAEDFTLELSGGRVMDADITVFDPL
                     TVEDQATYQNPYQPSAGIRHVIVNGVFVVRDEQFVENTFPGRRISSSL"
     gene            829..1350
                     /gene="thpR"
                     /locus_tag="AC16QF_01780"
     CDS             829..1350
                     /gene="thpR"
                     /locus_tag="AC16QF_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HIR6447310.1"
                     /translation="MRCFVACFLSAESAHRLGTRLEAAWTPPARARLVPRANYHVTLR
                     FFGEVCSAEVSGLLAAVAELGGHDLSCTTGPVTGLPRPARARVIAVGVEPSSPLTDWA
                     EALSGIDPEPEGRAFLPHVTLARIRGTMRVPQIGGLAGVELALHAPSLYETVPVDGGV
                     RYRPVEPERFGSG"
     gene            complement(1342..2361)
                     /gene="mtnA"
                     /locus_tag="AC16QF_01785"
     CDS             complement(1342..2361)
                     /gene="mtnA"
                     /locus_tag="AC16QF_01785"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIR6447311.1"
                     /translation="MNDPGFPPSAISWQDGRLRLLDQRRLPEEVAYLEIRTVDAAHEA
                     IRTLAVRGAPAIGIAAAYTLVVAMVADAPTDAAGFRARLAEHARHLTSARPTAVNLAW
                     ACARLLSAAERSADLTTIQAEAEAIHEEDRLICRAIGSHGQPLIPDNAGVLTHCNAGA
                     LAVSELGTATAPMYLSHAAGKPFRVFVDETRPLLQGARLTAWELAAAGMDVTLICDSA
                     AAAFMAAGEIDLAIVGTDRVTANGDVVNKIGTLGVAVLCRHFGIPFYVALPSSTFDPH
                     TATGAQVPIEERDPAEVLGDRAADVPARNPAFDVTPANLVTAFITDRGVLRPPFAESL
                     SSLSA"
     gene            complement(2363..2992)
                     /locus_tag="AC16QF_01790"
     CDS             complement(2363..2992)
                     /locus_tag="AC16QF_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012804.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR6447312.1"
                     /translation="MAEQEGVIRFAYDLQSPDGVVTEESLRETLAAWRTIFRRLGVLG
                     HDPDRYGGLGFGNLSVRDREHPEEFVVTASQTSGRSGLRDRDLVRITGCNLERFWVDA
                     VGHKPPSSETLTHAMIYAADPRIQWVFHVHSPEIWRHAEQLAIPCTAPEVAYGSADMV
                     RAVAELLEKHQSRPLLFATLGHEDGIFACGPTARDAGGLLVSYLAKALA"
     gene            3075..3926
                     /locus_tag="AC16QF_01795"
     CDS             3075..3926
                     /locus_tag="AC16QF_01795"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6447313.1"
                     /translation="MSGGSRRSWRAMTGALGAIAPPGDALDLPAAEIVQALNAHQVVA
                     FRNQSLDPAELVAFASRLGELDVYPLAEPLPESPFVVAVLKNPEDTSNFGGAWHTDTS
                     YVREPPKFTVLYALEIPGSGGDTLFADMYQAFAGLSPGLQRCLERVQAVYTADLVHAG
                     SGEYTAVAGQSVPAKESERATTVEHPVVRVHPETGRKALYTSLIHTERFVGMTREESL
                     PLLEYLQSVSVAAENCTRLHWQAGTLAIWDNRCVQHYPLNDYPGERRLMHRVIVRGEE
                     PVGVEPL"
     gene            4010..4540
                     /locus_tag="AC16QF_01800"
     CDS             4010..4540
                     /locus_tag="AC16QF_01800"
                     /inference="COORDINATES: protein motif:HMM:NF049802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:4295..4297,aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HIR6447314.1"
                     /translation="MVDYHDPRGEVATPVDAYLLSVDLESSNDPRVAFLANGFPDSDV
                     FLEQLAEVMSEKLTSLRAEHYNKGNASIAAPQDMLEEIREGCVAAVAAYGHUGSCTTG
                     TVRDGINMARLGIPAVALVTEDFWPQGDFIAGSLGMPDIPRVRLPHPVAGTGVANLRK
                     IAQEIAPEVFAALSRN"
     gene            4550..5611
                     /locus_tag="AC16QF_01805"
     CDS             4550..5611
                     /locus_tag="AC16QF_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447315.1"
                     /translation="MSGLFTARSEAAAVEHLHELGCTDGLPVIVPTPDRVERMVLASG
                     QDPAAVLGEMGPGKGIATIEKVAVAAVMAGCLPDYMPLVVAAVRAVIDPVFDLTEMQG
                     TTHCTAPLIIVNGPARHWCGPIASGFGALGPGHRANASIGRALRLTMINIGGARPGVS
                     DMALLGHPGKFTYCLAEDEEESPFTPLHVSRGFAAEDSVVTVIGAEAPHSVLYSVDAD
                     DPEDHEKLLGILAVGLGNLATNNAQLRNGSAVVVLNPDHAAVLANAGLSREDVAARLQ
                     ELAVHSRADIERYGKAFMNWSRDEEVFHCFQDPAQILVVMAGGSGLYSMVMPSWCAGG
                     HRNSPVSVRVELDQACEIP"
     gene            5693..6856
                     /locus_tag="AC16QF_01810"
     CDS             5693..6856
                     /locus_tag="AC16QF_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR6447316.1"
                     /translation="MNWPKDRYGFETRAIHAGQAPDPATGAVVTPVYLTSTFQHSEPG
                     EFAGFDYSRAGNPTRKAFEDCVANLEGARFGFAFASGCIGAATVVHLLEQGDHVVACD
                     DMYGGTHRLFEQVFRPQGIDFSYLDLSSADRLRGALRPETRMVWIESPTNPLMKLVDI
                     AAVATIAREHGAMLMVDNTFMTPYLQRPLELGADMVLHSTTKYINGHSDLIGGLVVTD
                     DEQLAERLDFLNKTMGGIQGAFDSYLCLRSVKTLAVRMQAHERNARAVAEMLASHPKV
                     EWVGYPGLSSHPQQELARRQASGFGGMVAFRVTGGLAAARRLLGAVEVFTLAESLGGV
                     ESLIEHPGLMTHASLPADRRAALGIDDGLIRVSVGIETTGDLLADLEAALAAA"
     gene            6856..7284
                     /locus_tag="AC16QF_01815"
     CDS             6856..7284
                     /locus_tag="AC16QF_01815"
                     /inference="COORDINATES: protein motif:HMM:NF051508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7832 domain-containing protein"
                     /protein_id="HIR6447317.1"
                     /translation="MIYDDLDRHLPAAGAIERAVVPMGMYLAWCVNLQLTGSALEEEA
                     GTLLLRVRYREKTGSELLLAACGGVLDGRWLNDAGRRFTDGYYGGYLDDFRSVFGSDV
                     YGVEDDWDHYDRLAPLLTRRYLGESAKTDRGARRWWKFWQ"
     gene            7303..7716
                     /locus_tag="AC16QF_01820"
     CDS             7303..7716
                     /locus_tag="AC16QF_01820"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6447318.1"
                     /translation="MSGRSNDGGGREFRVTDVRVYRPYPDEVPWDLLDRQAPADPAGD
                     GVQMRIAKLGDHVVGAYVIEPVDPLTFRIVDLVVAEGWRRRGIGRWLLGHAIGLAESR
                     GAREVRVTNPSPRRFFASIGFLDADAGMRFPITPE"
     gene            complement(7703..8863)
                     /locus_tag="AC16QF_01825"
     CDS             complement(7703..8863)
                     /locus_tag="AC16QF_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR6447319.1"
                     /translation="MVADIIAALRCGPFANDKGANDMSSDTLPKGLEGVEVDETGISL
                     VDGERGVLSYRGIDVDELVDKPFDEVAALVATGRLDPDFGARLAASAELSEREAGLVR
                     SLPRDVHPMHVVQGLTPLLDRDGAFADFGDAAQGFAVAAKLPALIATHMLGTAVAGYP
                     TLGHAERFLAQIAAPQTDIARRAFNVTQILQIEHGFNASTFAARVAASTLAPVENALS
                     AGFGTLHGRLHGGADQAALEVAEQVGTAAAAAAFVDRCIADGTRVMGMGHREYRVLDP
                     RARHIKELARSLAAGTPHEKTFETLAAIEDRFRERMAEKGKALHANVEFYKGVVYRVL
                     GMTPAFFTTGFAMARVFGYLAHFIESREDNRLIRPKARYTGPPVGAVRLTPA"
     gene            8962..9843
                     /locus_tag="AC16QF_01830"
     CDS             8962..9843
                     /locus_tag="AC16QF_01830"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6447320.1"
                     /translation="MQIERHEIRIFSAVVEEGGFSRAAERLNITQSAVSQAVANLEHK
                     LDARLLERRGKPELTEAGKRLFAFTQAVIKEETNALDDIAQIRAGALSTLNLGMSSMF
                     NRLFGRELLLEFCERNPLTRLKLDVAPSRELIYGVDEDRWELGIGPFQTLMPGHFETK
                     RFFEEERVLVAHEKHPRFDALMSQPARELAQLTLLTSYLDDATKRPGSERLRNQFASV
                     WEISSLELRLTLAEAGMGVTYVSDRLVGDLEGFHPIDHMEISTIPRVVGIFHKKHVAL
                     SEGAKRFLAICQRRFAG"
     gene            complement(9854..10462)
                     /locus_tag="AC16QF_01835"
     CDS             complement(9854..10462)
                     /locus_tag="AC16QF_01835"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HIR6447321.1"
                     /translation="MNIQERMRAVLQAEAEAIAAVRIDETFEHAVRALLECRGKVITT
                     GIGKAGHIARKFAATLCSGATPADFIHPAEAAHGDLGLVGPDDVMIAFSTSGKSREVL
                     EILELARHLGLAMVIGITSHPDSELRDLSDLVLDMGEIEEPCPVGLTPSASTAVMLAI
                     SDAIALALMEAKGITKEDWGLRHHGGYLGRAARADNVPHDGG"
     gene            10596..12611
                     /locus_tag="AC16QF_01840"
     CDS             10596..12611
                     /locus_tag="AC16QF_01840"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012931.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004563 - beta-N-acetylhexosaminidase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HIR6447322.1"
                     /translation="MPYIAGPEGALLPLPRRFAPGPGGPGAVGVVDARFADASSARLV
                     RAVERLNDRLGRRNGEAGLKMALEISCAIPCPEHPQLGQPEAYQLNVTDQGARLVAET
                     EWGVLHGLETLFQLATAPGGLGCVAVDDAPRFPWRGLLLDVARHYLGMDALLRTLDAM
                     AQVKLNVLHLHLTDDQAFRFPSERFPELASPRHYSRRELAELINHAADRGIRVVPEID
                     VPGHTTCWLVARPDWGTGVTTSTRRFGVHEACLDPTSEAVYAALAELLGEVAEVFPDR
                     HLHLGGDEVHPAWWSASDRVQTFMSERGMTGLDDMQAHFNSRVARTVSGLGKRPMGWD
                     EVVHDDLPDDVVVQSWRGATARDRALGSGHDCVVSSGYYLDLFYPADVHYRFDPQAPE
                     SELVGLEDALLDDPRFEHVAEGMRWTEHWREPAGAAGAAPGSVLGAEACLWGELVTEE
                     LLDVRLWSRLPALAERFWSPRSVRDLGSLYQRLGSCFEQLAAGSGVDVRAASRALMAR
                     AGADEDVVELLELLEPVKWYGRLLGEEALAARIAGTEMPQARPYDADTPLDRVVDALF
                     PESLPARRVARLCQRHFEGDTGAVAGILDCALAWQDLGPRPDCPADVAPALELLCRVG
                     EAVVDVLEGCMAPSAALSLLETAARPQGEYLIAVVPPLRSWLAGLGG"
     gene            12608..>12976
                     /locus_tag="AC16QF_01845"
     CDS             12608..>12976
                     /locus_tag="AC16QF_01845"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6447323.1"
                     /translation="MIDPLQVARSGWRVGKEVDRLGAGHINDTYLVTDGVDRWVLQRI
                     NDQVFTDPALLMRNLGRVVEHVCARAPGFVPGLLSCRDGSNVFVDEEGGSWRLTEYVS
                     GTESHRRLESVDQATSAARAF"
BASE COUNT         2008 a         4265 c         4520 g         2183 t
ORIGIN      
        1 cgaaggcgac ggcgggatga atcgcgcccc cccgcggggg cgagcaggcg accaccgtcg
       61 gcacgcctgc caccttcgcg gtggttacgc tcatcagcgc agacgctgcg tgggcgtagc
      121 gcccaccggg cacgtagcat ccggcgacct gaaccgggac gatgcgctgg ccgagggtga
      181 ccccgggctc ggtctggatt tcgaactcgt gcaggctcgc ccgctgggcc tcggcgaagc
      241 gcttgatctg gcgccaggcg aaggtgatgt cgtcgcgggt ttctgagggc agctgggcga
      301 tcagccgatc gcgcgcctcc cgggaaagga cgaagtcccc gtcgaacccg tccaggtccc
      361 gggcgtatcc ggcaacggca ggctccccct cactgcgtat cttcgccagc atctccgcga
      421 cgcgtaccgc gatgccctcc tcgtccggcg ccgcggccgg ctcggcccgc ttgagatagg
      481 tcgtcgccat cacaggctgc tgctgatgcg gcggcccggg aacgtgttct ccacgaactg
      541 ctcgtcgcgg accacgaaca cgccgttgac gatgacgtgt cggatgccag ccgagggctg
      601 atacggattc tgataggtgg cctggtcctc gacggtgagc gggtcgaaga cggtaatgtc
      661 cgcatccatg acgcggccgc cgctcagctc caacgtgaag tcctcggcgg cctggaccgc
      721 gctggcaatc agccagaggg ccggtatgcg gataactccg ttcgccatgc cgcgtctccc
      781 gcgcgccgat catgaaggcg caagatagaa cggtagaatg gggatgtcat gcgctgtttc
      841 gtagcctgct ttctgtctgc cgaatccgct caccggctcg ggacgcggct cgaggcggcg
      901 tggacaccgc ccgcccgggc gcgtctggtc ccgcgagcga actatcacgt cacgctgagg
      961 ttcttcgggg aggtctgctc tgccgaggta tccgggctgc tggctgcggt ggccgaactc
     1021 ggcggtcatg acctttcgtg cacgacgggg ccggtgacgg gtcttccgcg tccggcgcgt
     1081 gcccgcgtca tcgccgtggg cgtggagccc tcgtcgccac tgacggattg ggcggaagcg
     1141 ctgtcgggta tcgatccgga acccgaaggc cgggcgttcc tgccccacgt caccctggcc
     1201 cggattcgcg ggaccatgag ggtgccgcag atcggtggcc tcgcgggggt ggagctcgcg
     1261 cttcacgcgc cttcgctgta cgagaccgtt cccgtggatg gcggtgtgcg ctaccggccc
     1321 gtagagccgg agcggtttgg gtcaggctga caagctgctc aacgattccg caaacggcgg
     1381 tcgcaatacg ccgcggtcgg tgatgaacgc ggtcaccaaa ttcgctgggg tcacgtcgaa
     1441 agcgggattg cgggcaggca cgtccgcggc ccgatcgccc agcacctccg ccggatcgcg
     1501 ttcctcgatg ggtacctgcg cgcccgtcgc ggtgtgcgga tcgaacgtgc tcgagggcag
     1561 cgccacgtag aagggaatgc cgaagtgccg gcagagcacg gcgacgccca aggtgccgat
     1621 cttgttcacc acgtcgccgt tggcggtgac gcggtctgtc cccacgatgg ccaggtcgat
     1681 ctcccccgcg gccatgaagg cggctgctgc gctgtcgcag atgagcgtga cgtccatgcc
     1741 cgcggcggca agctcccagg ccgtcagacg cgcgccctga agcagcggcc tggtttcgtc
     1801 gacgaacacc cggaacggct tgccggcggc atggctgaga tacatgggag ccgtggcggt
     1861 gcccaactcg gaaaccgcca gagcaccagc gttgcagtgg gtgagcactc ccgcgttgtc
     1921 gggaatcaga ggctggccgt ggctgccgat ggctcgacag atgagacggt cctcctcgtg
     1981 gatcgcctcc gcctccgcct ggatggttgt gagatcggcc gagcgctcgg cggcgctcag
     2041 aagccgagcg catgcccacg cgaggtttac cgccgtgggg cgtgcggagg tgaggtggcg
     2101 agcgtgttcg gcgagccggg cgcggaagcc ggcggcatcc gtcggcgcgt cggcgaccat
     2161 ggcgactacc aaggtgtagg cggcggcgat gccgatggcg ggggcgcccc tcacggccag
     2221 ggtgcggatg gcttcgtgcg ccgcatccac cgtgcggatc tcgagatagg cgacctcctc
     2281 gggaagccgt cgctgatcga gcagccgcag gcggccgtcc tgccaggaga ttgcgctcgg
     2341 tggaaaccca gggtcgttca tgtcaggcca gcgctttggc caggtagctc accagcagcc
     2401 cgcccgcgtc gcgcgccgtg gggccgcagg caaagatccc gtcctcgtgg cccagggtgg
     2461 cgaatagcag cgggcgtgac tggtgctttt cgagcagctc ggccacggcg cgaaccatgt
     2521 ccgccgagcc gtaggccacc tcaggcgccg tgcaggggat tgcgagctgc tcggcgtgcc
     2581 gccatatctc cgggctgtga acgtggaaga cccactgaat ccgaggatcg gcggcgtaga
     2641 tcatggcgtg ggtgagcgtc tcagacgacg gcggcttgtg ccccaccgcg tctacccaga
     2701 aacgctccag attgcatccg gtgatccgca ccaggtcccg gtccctgagc ccgctgcggc
     2761 cgctggtctg gctggcggtg acgacgaatt cctccggatg ctcccgatcc cggacgctca
     2821 ggtttccgaa acccaaaccg ccgtagcggt ccggatcgtg gccgagaacg cccaggcgac
     2881 ggaagatggt ccgccacgcc gcgagggtct ctctcagcga ctcctcggtt acgacgccat
     2941 cgggcgactg aagatcgtag gcaaagcgga tgacgccttc ttgctcggcc actcgtgtaa
     3001 tatccggcga tgttcggtca cgcaaagcat gccacgatga tgcgggaaaa agcagcacat
     3061 gccgggagct ctgcgtgagc ggcggatccc ggcgtagctg gcgcgccatg acgggcgcgc
     3121 tcggcgcgat cgcgccgcca ggggatgccc ttgatctgcc ggccgccgag atcgtccagg
     3181 cactcaacgc ccatcaggtg gtcgcgttca ggaatcagtc tctcgacccg gcggagctgg
     3241 tggcgttcgc ttcccggctg ggtgagctcg acgtttaccc gctcgcagag ccccttcccg
     3301 aatcgccttt cgtggtcgcc gtgctcaaga acccggagga tacgagcaac ttcggcgggg
     3361 catggcatac ggacacctcc tacgtccgcg agccgcccaa gttcaccgtg ctctacgcct
     3421 tggaaattcc cggcagtggc ggtgacacgc tgttcgcgga catgtaccag gcgtttgccg
     3481 gcctgtcccc cgggctgcag aggtgccttg aacgcgtgca ggccgtgtat acggccgacc
     3541 tggtgcacgc gggcagcggg gagtacacgg cggtggcggg gcagagcgtt ccggcgaagg
     3601 agagcgagcg ggccaccacg gtggagcacc cggtggtgcg cgttcatccg gaaaccgggc
     3661 gcaaagcgct ctacaccagc ctgatccaca ccgagcggtt cgtcggcatg acgcgcgagg
     3721 aaagccttcc cctgctggag tacctgcaga gcgtttcggt ggccgctgaa aactgcaccc
     3781 ggctccactg gcaggccggc acgctggcca tctgggacaa tcgctgcgtg cagcactatc
     3841 cgctgaacga ctatcccgga gagcggcggc tcatgcaccg ggtaatcgtc aggggagaag
     3901 agcctgtggg cgtggaaccg ctctgaggcc cattgctgag ctggagttcg ctgcccattc
     3961 ccgttttaat cggtgtcatc gatcagcacc ctgcatcgga ggtgggtata tggttgacta
     4021 tcacgacccc cggggagagg tcgcaacgcc ggtggatgcc tatctgctga gcgtcgatct
     4081 cgagtccagc aacgacccgc gtgtggcgtt cctcgccaac gggttcccgg attcagacgt
     4141 ttttctcgag cagctcgccg aagtcatgag cgaaaagctg acgagccttc gcgccgagca
     4201 ctacaacaag ggcaacgcgt ccatcgccgc gccccaggac atgctcgagg aaattcgcga
     4261 gggctgcgtg gcggcggtgg ccgcctacgg ccactgaggc agttgcacta ccggcaccgt
     4321 gcgtgacggc atcaacatgg caaggctcgg catcccggcg gtggcgctgg tgaccgagga
     4381 cttctggcct cagggcgact tcatcgccgg ctcgctcggc atgcccgaca ttcccagggt
     4441 tcggctgccc cacccggtgg cggggaccgg tgtcgccaac ctgcgcaaga ttgcgcagga
     4501 gatagcgcct gaggttttcg cggcgctgtc ccggaactag ggcgaggctt tgagcggatt
     4561 gttcacggcc cggagcgagg cggccgcggt cgagcacctt cacgagctcg gctgcaccga
     4621 cggcctgccc gtcatcgtgc ccacgccgga cagggtggaa cgcatggtcc tggcctccgg
     4681 ccaggatccc gccgccgtcc tcggtgagat gggtccgggc aagggcatcg ccaccatcga
     4741 gaaggtggcg gtggcagcgg tgatggccgg ctgcctcccg gactacatgc cgttggtggt
     4801 ggccgccgtg cgggcggtca tcgatcccgt cttcgacctc acggagatgc aggggaccac
     4861 ccactgcacg gcgcccctga tcatcgtcaa cgggcccgcg cggcactggt gcggtcccat
     4921 cgccagcggc ttcggcgccc tgggaccggg gcatcgggcc aacgcctcca tcgggcgagc
     4981 gctgcggctc acgatgatca acatcggcgg cgcccgcccc ggcgtctccg acatggccct
     5041 gctcggccat cccggaaagt tcacctactg tcttgccgag gacgaggagg agagcccgtt
     5101 tacccccctt cacgtttccc ggggcttcgc ggccgaagac agcgtggtga ccgtcatcgg
     5161 cgcggaggcg ccgcactccg tgctttacag cgtcgatgcc gacgatcccg aggatcacga
     5221 gaagctgctc ggcatcctcg ccgtgggcct gggcaacctg gctacgaaca acgcccaact
     5281 gcgcaacggc tccgccgtgg tggtgctaaa cccggatcat gctgccgtgc tcgccaatgc
     5341 gggcttgagt cgcgaggacg tggccgcccg cctgcaggag ctggccgtgc actcccgtgc
     5401 cgatatcgag cgctacggca aggcgttcat gaactggtcc agggacgaag aagtatttca
     5461 ctgctttcag gaccccgcgc agatcctcgt ggtgatggcc ggtggcagcg ggctgtattc
     5521 catggtcatg ccctcctggt gcgcgggagg ccacaggaac tctccggtca gcgtcagggt
     5581 cgaactcgac caggcctgcg agatcccctg acctgccgcg agccatgccg tcggaggtgc
     5641 tccggccggg ggcgatgggt cgccggaggc aaaggtttca gggagctgca gcatgaactg
     5701 gcccaaagac agatatggat tcgagacccg ggcgattcac gcggggcagg cccccgaccc
     5761 cgcgacgggc gccgtggtga cccccgttta cctgacctcg acctttcagc actccgagcc
     5821 aggcgagttt gccggcttcg attactcccg ggccggcaac ccgacccgca aggccttcga
     5881 ggactgcgtt gccaacctgg aaggggcgcg gttcggcttc gcctttgcgt ctggctgcat
     5941 cggcgcggcg acggtcgttc acctgctcga gcagggcgat cacgtggtgg cctgtgacga
     6001 catgtatggc ggcacccacc gcctgttcga gcaggtattc cggccccagg ggatcgactt
     6061 cagctacctc gatctaagct cggcggatcg gcttcgcggg gcgcttcgac cggagacccg
     6121 catggtctgg atcgagtcgc ccaccaatcc tctgatgaag ctcgtggaca ttgccgccgt
     6181 tgcgacgatt gcccgcgaac acggcgcgat gctgatggtg gacaacacct tcatgacccc
     6241 ttatctccag cgcccgctgg agctcggggc cgacatggtg ctgcactcca ccacgaagta
     6301 catcaacggt cacagcgacc tgatcggcgg gctggtggtc accgacgacg agcagcttgc
     6361 cgagcgcctc gactttctca acaagaccat gggcggcatc cagggcgcgt tcgacagcta
     6421 cctttgcctg cgcagcgtga agaccctggc cgttcgcatg caggcacatg agcgaaacgc
     6481 gcgggcggtt gcggagatgc tggcgtcgca cccgaaggtg gaatgggtgg gctaccctgg
     6541 gctgtcgagc catccgcagc aggagctggc tcggcggcag gcctcgggct tcggcgggat
     6601 ggtcgccttc cgcgtcacag gtggcctggc tgctgcccgg cgcctgctcg gcgcggtgga
     6661 ggtgttcacc ctggccgaga gcctgggtgg cgtcgagtcc ctcatcgagc atccgggcct
     6721 gatgacccac gcgagcctgc cggccgatcg ccgagccgcg ctcgggatcg acgacgggct
     6781 catccgcgtc agcgtgggga tcgagacgac gggcgatctc ctggccgatc tcgaagctgc
     6841 gctggctgcc gcctagtgat ttacgacgat ctggaccggc atctgcccgc tgccggcgcc
     6901 attgagcgcg ccgtggtgcc catgggcatg tacctggcat ggtgcgtcaa tcttcagctg
     6961 acgggcagcg cgctggagga ggaggccggg acccttttgc tgcgggttcg ctaccgggag
     7021 aaaaccggta gcgagctgct gctcgccgcc tgcggcggcg tgctggacgg ccgctggctg
     7081 aacgacgcgg gccgtcgctt cacagacggc tactacggtg gatatctgga cgacttccgc
     7141 tcggttttcg gatccgacgt ctacggcgta gaggacgact gggatcacta cgatcgtctt
     7201 gcgcccctgc tgacgcgccg gtacctcgga gagagcgcta agacggatcg cggcgcccgg
     7261 cgatggtgga agttctggca atgatttggc gatgagctgg caatgagcgg taggtctaat
     7321 gacggtggcg gacgggaatt ccgggtaacc gacgtgcgcg tctaccggcc ttatccagac
     7381 gaggttccct gggacctgct ggatcggcag gcaccggcgg atcccgcggg agacggggtg
     7441 cagatgcgca tcgcgaagct cggcgaccac gtggtgggcg cttacgtcat cgaacccgtc
     7501 gatccgctga ccttcaggat cgtggacctg gtggtggccg aaggctggcg ccggcgcggg
     7561 atcggcagat ggcttctggg tcacgccatc gggctggcgg agtcccgtgg ggctcgcgag
     7621 gttcgggtga ccaatccatc ccctcggcgg ttcttcgcca gcatcggttt cttggacgcc
     7681 gatgcaggga tgcgatttcc catcacgccg gagtgagcct cactgcgccc acgggcgggc
     7741 ccgtgtaccg agctttcggg cggatcaggc ggttgtcctc gcggctctcg atgaagtggg
     7801 ccaggtagcc gaacacccgg gccatggcaa agcccgtggt gaagaacgcg ggcgtcatgc
     7861 caagcacccg gtagacgacg cccttgtaga actccacgtt ggcgtggagc gctttgccct
     7921 tctcggccat ccgctcgcga aaccgatcct cgatggccgc taaggtctcg aaggtcttct
     7981 cgtggggcgt gccggcggcg aggctgcgcg cgagttcctt gatgtggcgc gcgcgtgggt
     8041 ccagcacgcg gtattcccga tgccccatgc ccatcacgcg ggtcccgtcg gcgatgcatc
     8101 ggtccacgaa cgccgccgca gccgccgctg tgcccacctg ctcggcgacc tccaacgccg
     8161 cctggtccgc gccgccgtgc agacgcccgt ggagggtccc gaagccggcg gacagggcat
     8221 tttccaccgg tgcgagggtg ctcgccgcga cccgggcggc aaaggtgctg gcgttgaagc
     8281 cgtgctcgat ctgcaggatc tgggtgacgt tgaaggcgcg ccgggcgatg tcggtctgcg
     8341 gggccgcgat ctgggccagg aaccgctccg cgtgccccaa tgtcggatag cccgccaccg
     8401 cggtgccgag catgtgcgtc gcgatcagcg caggaagctt ggccgccacg gcaaatccct
     8461 gagcggcgtc gccgaagtcg gcaaaggccc cgtcgcggtc aagaagcggc gtgagtccct
     8521 gcactacgtg catgggatga acgtcgcgcg gcagcgagcg gaccagcccc gcctcgcgct
     8581 cggacagctc cgccgaggcc gccaggcgcg cgccgaaatc gggatcgagc cggcccgtcg
     8641 ccacgagcgc cgcgacctcg tcgaaaggct tgtcgaccag ctcgtccacg tcgatgcctc
     8701 ggtagctcag gaccccgcgc tcaccgtcca ccagcgagat cccggtctcg tccacctcca
     8761 cgccttcgag ccctttgggc agggtgtcgc tcgacatgtc gttggctcct ttgtcattcg
     8821 cgaacggccc gcagcgtagc gcggcgatga tgtcggcgac catccgcttt cacttctccg
     8881 gagatcaggc cggcttatcc attagcccag ataatggctc tataagtgga gcgcccccgc
     8941 aggccctggt agcctccgga catgcagatc gagcgccacg aaatcaggat cttcagcgcc
     9001 gtggtggagg aaggcggctt cagcagggcc gccgagcgtc ttaacatcac ccagtcggcg
     9061 gtcagccagg cggtggcgaa tctcgagcac aagctcgatg cccggctgct cgaacgccgc
     9121 ggcaagccgg agctcacgga ggcgggcaag cggctgttcg cgttcaccca ggccgtgatc
     9181 aaagaggaaa ccaacgcgct ggacgacatc gcgcagattc gcgccggcgc gctttcgacc
     9241 ctcaatctgg ggatgagcag catgttcaac cggctgttcg gccgcgagct gctgctcgag
     9301 ttctgtgagc gcaacccgct gacccggcta aagctcgacg tggcgccgag ccgcgagctc
     9361 atctacggcg tcgacgagga tcgctgggag ctcggcatcg ggccgttcca gaccctgatg
     9421 cccggacact tcgagaccaa acgattcttc gaggaggagc gcgtgctggt ggcgcacgag
     9481 aagcacccgc gcttcgacgc gctcatgagt caacccgccc gcgagctggc tcagctgacc
     9541 ctgctcacct cgtatctgga cgacgccacc aagcgcccgg gcagcgagcg ccttagaaac
     9601 cagtttgcga gcgtctggga gatcagcagt ctggagctgc gcctgactct ggcggaggcg
     9661 ggcatgggcg tgacctacgt ttccgaccgg ctggtcggcg acctggaggg gttccatccc
     9721 atagatcaca tggaaatctc gaccattccc cgagtggtgg gcatcttcca caagaaacac
     9781 gttgcccttt cggaaggcgc caagcgcttt ctggccatct gccagcggcg gtttgccggc
     9841 tgattcgagc gggtcagccg ccgtcgtggg gcacgttgtc ggcgcgggcc gccctgccca
     9901 gatagccgcc gtgatggcgc aagccccagt cttccttggt gatgcccttg gcctccatga
     9961 gcgccagcgc aatggcgtcg ctgatggcga gcatgacggc cgtgctggcg ctgggggtca
    10021 gccccaccgg gcaaggttct tcgatctcgc ccatgtccag caccaggtcg gaaaggtcgc
    10081 gtagctcgga gtcggggtgg gaggtgatgc cgatgaccat ggccaggccc aggtgcctgg
    10141 ccagctcgag tatttccagc acctcgcggc tcttgccgct ggtcgagaag gcgatcatca
    10201 cgtcgtcggg tcccaccagc cccaggtccc cgtgagcggc ttcggcaggg tggatgaagt
    10261 ctgccggcgt cgcgccggag cagagcgtgg cggcaaactt gcgggcgatg tgacccgcct
    10321 tgccgatgcc cgtggtgatc accttgccgc ggcactcgag cagggcgcgg acggcgtgct
    10381 caaacgtctc gtcgatccga accgccgcaa tggcctcggc ctcggcctgc agcacggccc
    10441 gcatacgttc ctgaatgttc attgccgaca cggtccgcga ttgcccggcg caatggtgtc
    10501 acgcgtatcc ggcgtcctca aggtgacgcc gtgcattgac agcgtctggc gcctcttaca
    10561 gagtccgggt ctcttcgacg ttcgaggcac gacgcttgcc gtacatagcg ggaccggaag
    10621 gggcgctgtt acccctgccg cgccggtttg ccccggggcc tggtgggccg ggggcggtgg
    10681 gcgtcgtgga tgcccgcttt gccgacgcca gctcggcgcg gctggtacgc gcggtggaac
    10741 ggctgaatga ccgtctgggc cggcgcaacg gggaggcggg tctgaaaatg gccctcgaga
    10801 tctcctgcgc gatcccgtgt ccggagcacc ctcagctggg ccagccggag gcttaccagc
    10861 tcaacgtgac tgaccagggc gcccggctcg tggcggagac cgagtggggg gtcctgcacg
    10921 ggctggaaac cctgttccag ctggcgacgg cgccgggcgg cctcggctgc gtggctgtcg
    10981 acgatgcgcc cagatttccc tggcgcggtc tgctcctgga cgtggcccgg cactatctcg
    11041 gcatggacgc gctcctgcgt acgctcgacg ccatggctca ggtgaagctg aacgtcctgc
    11101 atctgcacct gacggacgac caggcgttcc ggtttcccag cgagcgattc cccgagctcg
    11161 ccagcccacg acactattcc cggcgagagc tcgccgagct catcaaccac gcggccgacc
    11221 gcggcattcg cgtggtgccc gaaatcgacg tccccgggca cacgacctgc tggctcgtgg
    11281 cccgccccga ctgggggacc ggcgtgacga cgtcgaccag gcggttcggt gtccacgagg
    11341 cctgcctgga tcccaccagc gaagccgtct acgccgctct ggccgagctg ctgggcgagg
    11401 tggccgaggt gtttccggat cgccatctgc atctgggggg cgatgaggtg catccggctt
    11461 ggtggtcggc aagcgaccgc gttcagacct tcatgtcgga gcgcggcatg accgggctcg
    11521 acgacatgca ggcccacttc aactcccggg tggcccgcac ggtgagtggg cttggcaagc
    11581 ggcccatggg ttgggacgag gtggtgcacg acgatctgcc cgacgacgtg gtcgttcaga
    11641 gctggcgggg cgcgaccgcg cgggaccggg ctctgggtag cggccacgac tgcgtcgttt
    11701 cgtcgggcta ctacctggac ctcttctatc cggccgacgt gcactaccgc ttcgatccgc
    11761 aagccccgga gtccgagctc gtcgggctcg aggatgcgct cctcgacgac ccccggttcg
    11821 agcacgtggc cgagggcatg cgctggaccg agcattggcg cgagcctgcc ggcgcagccg
    11881 gcgctgcccc cggttcggtt ctgggcgccg aggcgtgcct gtggggcgag ctggtcaccg
    11941 aggagctgct cgacgtgcgc ctgtggtccc ggctgccggc cctggccgag cgattctggt
    12001 cgccccgctc ggtgcgcgat ctggggagcc tttaccagcg gctgggaagc tgctttgagc
    12061 agctcgccgc gggctcgggg gtggacgtcc gggcggcgag ccgtgccctc atggcgcggg
    12121 cgggagcgga cgaggacgtc gtcgagctgc tcgagctgct ggaaccggtc aagtggtacg
    12181 gacggctcct gggcgaggaa gcgctcgccg cccgcatcgc gggaaccgag atgccccagg
    12241 cccggcccta cgatgcggac acgccgctgg acagggttgt cgatgcgctg tttccggaga
    12301 gcctgcccgc gcgccgtgtg gccaggctgt gccaacgtca cttcgaaggc gacaccggcg
    12361 ctgtggcggg tatcctggat tgcgcgctgg catggcagga ccttggcccg cggccggact
    12421 gcccggccga cgtcgcgccc gccctcgagc tcctctgccg cgtgggcgag gcggtggtgg
    12481 acgtgctcga aggttgcatg gcgccttccg ctgcgctgtc gctgctggag acggctgcca
    12541 ggccccaggg tgagtatctc atcgccgtgg tgccgcccct gcggtcgtgg ctcgcggggc
    12601 tgggcggatg atcgatccgt tgcaggtggc ccgctccggc tggcgagtcg gcaaggaggt
    12661 cgatcgcctg ggtgccggac acatcaacga cacgtacctg gtgaccgacg gcgtcgaccg
    12721 ctgggtcctg cagagaatca acgaccaggt gtttacggac ccggccctcc tgatgcgcaa
    12781 cctgggccgg gtagtggaac acgtttgcgc tcgcgcgccg ggattcgtgc ccgggctgct
    12841 gtcgtgccgc gatggctcga acgtcttcgt ggacgaggaa ggcggcagct ggcgcctgac
    12901 ggagtacgtg agcggaaccg aaagccaccg tcgtctcgaa agcgtcgacc aggcaacgtc
    12961 agcggcgcgt gcgttc
//
LOCUS       DBNHLM010000039         4290 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1621920, whole genome shotgun sequence.
ACCESSION   DBNHLM010000039 DBNHLM010000000
VERSION     DBNHLM010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1621920"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="AC16QF_01850"
     CDS             complement(<1..832)
                     /locus_tag="AC16QF_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HIR6447324.1"
                     /translation="MVSITLPDGSQRQFDGSVTGTELARDISKSLAKAAVAVRVDGEL
                     KDIYIPIEQDAAVEVITRDSQAGVDLLRHDAAHVLAEAVKELYPETQVTIGPTIENGF
                     YYDFSRAEPFTPEDLETIEARMREIVARNEEIRREVWDRDEAVAYFQSIGESYKAEII
                     AGIPEGEEVGLYRQGEFLDLCRGPHLESTGRLGGAFKLMSVAGAYWRGDSRNEMLQRI
                     YGTAWATDKDLNAYLVRLEEAEKRDHRRLGREMDLFHLQEEGPGAVFWHPKGWTMFQR
                     M"
     gene            complement(903..2489)
                     /locus_tag="AC16QF_01855"
     CDS             complement(903..2489)
                     /locus_tag="AC16QF_01855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR6447325.1"
                     /translation="MDAYDYAIVGAGSAGCVLANRLSADPETRVLLIEAGPGNGNPLL
                     HIPAGWATNFRNPKVDWGFSTEPEPALNGRSIYWPRGKVLGGSSAINGMIYIRGVPLD
                     FDRWAQSGARGWSYEEVLPYFRKAERQQRVADEFHGAEGPLHVQDVRDRRAAMDAFID
                     GMTELGIPRNDDFNGQEQAGCGYYQFNQLRGRRWSTATAYLGPARRRPNLDVMTGVLV
                     RRVCFDGKAATGLEIERARSPATRVAAREVILASGAINSPQLLELSGVGDGERLRGLG
                     IEVVGDLPEVGENLQDHLLTKTVYGTHPEQSINREVQGWRVGVAALKWLMLRRGPLTT
                     GSAPVGGFWYTRDGLDAPDVQVHFASGATLYNEEGRIRAMPVPAMTAVVNQSRPESVG
                     SIHITSADPRQPPAIRPNYLDSELDRDTLVRGLRMIIDIFESTAMRPYVTERLLPEPG
                     VTSDEALLEYIRGDAATTYHPTSTCRIGSVVDENLKVLGVSGLRVVDASVMPFVVSGN
                     TNAATIMIAEKAADLIRAGG"
     gene            complement(2528..3031)
                     /locus_tag="AC16QF_01860"
     CDS             complement(2528..3031)
                     /locus_tag="AC16QF_01860"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6447326.1"
                     /translation="MTLQATFNTSKGAIQVSLMPERAPVTVANFANLARRGFYDGLKF
                     HRVIADFMVQGGCPLGTGTGGPGYRFEDEFDSEVRHDRPGRLSMANAGPGTNGSQFFI
                     THVPTPWLDGNHTIFGEVQGPEDQAVVDSIAQGDVIESVVIEGDLDALLEAHADRIEE
                     WNRALDR"
     gene            3220..4257
                     /locus_tag="AC16QF_01865"
     CDS             3220..4257
                     /locus_tag="AC16QF_01865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6447327.1"
                     /translation="MRLLLVTDAWFPQVNGVVRTLSTTMENLARLGHETEVIAPARFK
                     SVPCPTYPEIRLALCTPGRVGNMIAEFAPDAVHVATEGPLGLAARWWLKRRGIPFTTS
                     FHTMFPQYVRLRFGLPEAWTFAAMRRFHGAATQTMYSTPRLRDMLRGQGFANLVGWVR
                     GVDTELFKPGPAEPLDLPRPLQMYVGRVAVEKSLEDFLSLDTPGTKVIIGDGPQRGEL
                     AAQYPQARFLGPRYGDDLVRCYRAADVFVFPSRTDTLGLVMLEAMACGVPVAAFPVQG
                     PLDVVADSGAGVLHDDLGTAIAQAIGIDPEICRARAMEHSWDQSVREFHANLVPIGQT
                     AYHFRGEGETA"
BASE COUNT          731 a         1444 c         1372 g          743 t
ORIGIN      
        1 tcatgcgctg gaacatggtc cagcccttgg ggtgccagaa cacggctccg ggaccctctt
       61 cctggaggtg gaacagatcc atttcccggc cgagccgccg gtgatcgcgc ttctcggctt
      121 cctccagccg caccagatat gcgttaagat ccttgtcagt cgcccatgcc gtcccgtaaa
      181 tacgctgaag catctcgttg cgggagtcgc cccgccagta agcgccggcc accgacatga
      241 gcttgaaagc accgccaagc cggcccgtgg actccaggtg cgggccccgg cagagatcca
      301 ggaattcgcc ctggcggtac agaccgacct cctcgccctc gggaatgccg gcaatgatct
      361 ccgctttgta ggactcgccg atggactgga agtaagccac ggcttcgtcc cggtcccaga
      421 cctcgcggcg gatctcctcg ttgcgcgcga cgatctcccg catgcgggcc tcgatggtct
      481 ccagatcctc tggcgtgaac ggctccgccc gggaaaagtc gtagtaaaag ccgttttcga
      541 tggtgggccc gatggtgacc tgggtctccg ggtagagttc cttgaccgcc tccgccagca
      601 cgtgggccgc gtcgtgacgc agaaggtcca caccggcctg ggagtctcga gtgatgacct
      661 ccaccgccgc gtcctgctcg atggggatgt agatgtcctt gagctcaccg tccacgcgaa
      721 cggccaccgc cgccttggcc agcgacttgg agatatccct ggccagctcc gtgccggtca
      781 ccgacccgtc gaactggcgc tgggaaccgt cgggcagggt gatggatacc attagaaaag
      841 acctggtggc cgaaaaacgg cgattttgcc aaacggcccc gccgcggtcg acgtgtaatt
      901 gatcagccgc ctgcccgaat gaggtctgcg gccttctccg cgatcatgat ggtcgccgca
      961 ttggtattgc ccgaaaccac gaaaggcatg acggacgcat ccacgacccg aagcccggag
     1021 acccccagaa ccttcaggtt ctcatccacc acggatccga tgcggcacgt gctcgtggga
     1081 tggtaggtgg tggcagcgtc gccgcggatg tactcgagca gcgcctcgtc agaggtgacg
     1141 ccgggttcgg gcaggagacg ctcagttacg tacggccgca tcgccgtgct ctcgaagatg
     1201 tcgataatca ttcgcagacc cctgacgagg gtatcccggt cgagctcgct gtcgagatag
     1261 ttgggccgga tggccggcgg ttgcctcggg tcggcgctgg tgatgtggat cgagcccacg
     1321 gactcaggcc gactctggtt gaccaccgcg gtcatggcgg gcaccggcat ggcccggata
     1381 cgcccttcct cgttgtaaag cgttgcgccg gaagcgaaat gaacctgcac gtcgggggcg
     1441 tcgaggccgt cacgcgtgta ccagaagccg ccgacaggag ccgatccggt ggtcaacggt
     1501 ccacgcctga gcatgagcca tttcaaggcg gcaacgccga cccgccaccc ctgcacctcc
     1561 cggttgatcg attgctcggg atgagtcccg taaaccgtct tggtcagaag gtgatcctgg
     1621 agattctcgc cgacctcggg caggtcaccg acgacctcga tgccgagccc ccgcagccgc
     1681 tcgccgtccc cgactccgga cagctcgagg agctgcggcg agttgatcgc gccgctggcc
     1741 aggatgactt ccctggcagc tacccgcgtg gcgggggatc gggcccgctc gatctccagg
     1801 cccgtggccg ccttcccgtc gaagcatacc cgcctgacga ggacgcccgt catcacgtcg
     1861 aggttgggcc ggcgtctggc gggaccgagg taggcggttg ccgtgctcca gcgccgaccc
     1921 cggagctgat tgaactggta gtaaccgcaa ccggcctgct cctgcccgtt gaaatcgtcg
     1981 ttgcgcggaa tgcccagctc cgtcatgccg tcgatgaacg catccatggc cgctcgcctg
     2041 tcgcgcacgt cctggacgtg caggggtccc tccgcgccgt gaaactcgtc ggccacccgc
     2101 tgctggcgct cggccttacg gaaatacgga agcacctcct cgtagctcca gccccgagcc
     2161 cccgactggg cccagcggtc gaagtccagg ggaacgccgc gaatgtagat catcccgttg
     2221 atggcgctgg agccacccag aaccttgccc cgaggccagt agatggagcg cccgttcagg
     2281 gccggctcgg gctcagtgga aaaaccccag tccaccttcg ggttgcggaa gttggtcgcc
     2341 caccccgcag gaatgtgcag cagcgggttc ccgttgcccg gccccgcctc gatcaggagc
     2401 acgcgcgtct ccgggtccgc cgacaaccgg ttggccagaa cgcagccggc cgagccggct
     2461 cccacgatgg cgtagtcgta ggcgtccatg accggtttcg cgcttcaggg gctcgtgacg
     2521 gggcggatca acggtccagg gcccggttcc attcctctat gcgatccgcg tgggcttcta
     2581 gcagagcgtc caggtccccc tcgatcacca cgctttcaat gacgtcgccc tgggcgatgc
     2641 tgtccaccac ggcctgatct tccggcccct gaacctcgcc gaagatggtg tggttgccgt
     2701 cgagccaggg agtgggtacg tgggtgatga agaactgcga gccgttggtg ccggggccgg
     2761 cgttggccat ggatagccga ccggggcgat cgtggcggac ctccgagtcg aactcgtcct
     2821 cgaagcggta gccgggaccg ccggtccccg tacccagcgg acagcctccc tgaaccatga
     2881 agtcggcgat cacgcgatgg aatttcagac catcgtaaaa accccgccgg gcaaggttcg
     2941 cgaagttcgc tacggttacc ggagcccgct cgggcatcag ggaaacctga atggctcctt
     3001 tggaagtgtt gaaggtggct tgcagagtca cgttagctcc tgtggggtaa cttgaagagg
     3061 ctcgagagcc gtatcggcgg ccagtgaaac acaacgggca ggcgttttcg agcggcgcca
     3121 cctgcccagg cggccaaacc cccagcgggt tgtagccgcc ctcccagtcc cgctactatc
     3181 ctcgtcaagc gaccgcaccg cggcgattag agagataacg tgcggctact gttagtcaca
     3241 gatgcctggt tcccacaggt gaacggcgtg gttcgaacgc tgtccaccac catggaaaat
     3301 ctcgcgcggc tgggccacga gaccgaggtg atcgcccctg cgcgcttcaa aagcgtcccc
     3361 tgtccgacct acccggagat ccggctcgcg ctgtgcactc cgggccgggt cgggaacatg
     3421 atcgccgagt tcgcgccgga cgcggtgcac gtcgccacgg aagggccact ggggctggcc
     3481 gcccgctggt ggctcaagcg ccgcggcata ccgttcacca cctcgttcca caccatgttc
     3541 ccgcagtacg tcaggctccg gttcgggctg cctgaagcat ggacgtttgc ggccatgcgc
     3601 cgcttccacg gggccgcgac tcagaccatg tactccacgc cccggctgcg cgacatgctc
     3661 cgcggccagg gcttcgccaa cctcgtcggc tgggtgcgcg gcgtggacac ggagctgttc
     3721 aagccgggtc cggcggaacc cctcgatctg ccgcggcctt tgcagatgta cgtcggccgg
     3781 gttgccgtgg agaagagcct cgaggacttt ctgtccctgg acacccccgg caccaaggtg
     3841 atcatcggcg acgggcccca gcggggagag cttgccgcac aatatcccca agcacgtttc
     3901 ctgggccccc gctacggcga cgacctggtc cgctgctacc gggcagccga cgtgttcgtg
     3961 ttcccatcgc gcaccgacac cctgggactg gtgatgctgg aagcgatggc ctgcggcgtg
     4021 ccggtcgctg cgtttccggt gcagggccca ttggacgtgg tcgccgacag cggcgcagga
     4081 gtgctgcacg acgacctcgg caccgccatc gctcaggcca tcggcatcga ccccgaaatc
     4141 tgccgagccc gggccatgga gcactcgtgg gatcagtccg tgcgcgaatt ccacgcaaac
     4201 ctggtaccca tcggacagac cgcctaccat ttccgcggcg aaggggagac cgcatgaaaa
     4261 ttctcattct cggcggggac ggcttctgcg
//
LOCUS       DBNHLM010000040         5240 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_843856, whole genome shotgun sequence.
ACCESSION   DBNHLM010000040 DBNHLM010000000
VERSION     DBNHLM010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_843856"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="AC16QF_01870"
     CDS             <1..463
                     /locus_tag="AC16QF_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR6447328.1"
                     /translation="SDRLECLIFGMGDYSASQQMQLGHIGSSGGYPPDVWHYPRYKMT
                     IACHANGIDPVDGPYANFRNSEAYGKEAERAFVLGMVGKWAIHPSQIELANAVFTPDP
                     AALADARKQKAAYNEALSQGLGAINVDGVMVDAASIRIVQNLLDRAELIGM"
     gene            467..1651
                     /locus_tag="AC16QF_01875"
     CDS             467..1651
                     /locus_tag="AC16QF_01875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6447329.1"
                     /translation="MTETYLTDERRMIQEVARDFTRREVLPVANELDPIQGDIPMDLR
                     DKLGEMGYFGIRMAEEHGGLGLGVFEYCLISEELARGWMSVASIIARATSLMNVGGWP
                     LEKREELTQKAARGEYLAAISLSEPDVGSDLAAAKCRAVLDGDEWVITGSKYWCTFAD
                     GADYIQLLARVEAPDSDDAQHKGLKNFIIEKPRGELPEGCSGSPIPKIGYFGWKTWEL
                     AFDGCRIPKENIITGGGGDGFKNAMHFLNAARAHTAARSIGLARGALEDAMAYSHERV
                     QFGVPIADFQETRFKIARMASEIEAARQLMYHAATLLDAGETAERESAMVKWYASEMA
                     ERVTSDALQILGGAGYTKLHAVERHWRDARLTKIFEGTSEIQLRIISDRLLGKPSHRA
                     TA"
     gene            1748..3157
                     /locus_tag="AC16QF_01880"
     CDS             1748..3157
                     /locus_tag="AC16QF_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR6447330.1"
                     /translation="MGDKDPGDLPTTFGELKSSGYQVLPVKEELRRNLISRLRSRETL
                     FPGVMGYDDTVVPQLTNAILGSQDIILLGERGQAKSRMVRSLTSLLDEWTPVLAGTEI
                     PENPFQPITAQGRQILEAQGDSAPIRWLHRDERYAEKLATPDITIADLIGEVDPIKVA
                     EGRYLSDELTLHYGLVPRVNRGIFAINELPDLSERIQVGLLNLLEERDVQIRGHKVRL
                     PLDVFIVATANPEDYTNRGRIITPLKDRYGAQIRTHYPADVDQEVAIMEQEAAVADMG
                     DYRLEIPAFMKEIVAEITHHARRSPDINQRSGVSVRTSIADYEAMLANAFRRALRLGE
                     DEVVPRISDLAFVLPSFQGKVEFEAMEEGQEDKITQRIMQAAIKSVFDRYYDVNDLES
                     IALAFNDGLTVDTGEATPAADYNAIGERIPGLMDVFPDREGVSPGVRAAIIEFILEGL
                     HLHKLLNKFDNRGAATYSG"
     gene            3188..>5240
                     /locus_tag="AC16QF_01885"
     CDS             3188..>5240
                     /locus_tag="AC16QF_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6447331.1"
                     /translation="MRHFPSGYRFSRWDGSQKLPLDADEIMAALADDLMEYGDLRWAM
                     RNLMSRGMQIPQGGYMQGLRDMLRQLRERKREQLERFDLSSVFEDFRERLDEILAMER
                     KRIDEWLDPKPATDSEAPAGNGPSGEPEQSTSADQPAAQGDGPDFSGDVLKQIAKRNR
                     DELDGLPPDLAGQVRTLEKYEFLNPDAQRKFLELLNELRRAMTNTFFNDIENMVNQLS
                     DGDVERMKEMIKALNEMLVKRIAGEDPGFEDFMAQYGDMFGDDPPQSLDDLLERMAQQ
                     MAAAQSLLNSMSPEQQQQLQSLLSDRFGDPELNSELRKLAKEMDFLNPDGGKYRFSGD
                     ERLDLETAMKLMSEMQDLDELISQVQEAERGGGTDRIDRDLLSDLLGDAAADDLEQLQ
                     QMLKALEEAGYIRPTDDDGWEITPRGSRMIGQKALGEIYARLKRQNLGNHAVPEEGRF
                     GERLEQTKAYEFGDPFHLHMPRTLRNAIDREGPQTPVSLKPEDFEIYRSELITSTATA
                     MLVDLSWSMALRGSFQAAKKVALALHNLITSQYPKDTFYIIGFAAYAKELKPKDLPYL
                     QWDEYVLGTNMQHALLLAERLLAKHQAGSKQIIMISDGEPTAHLENGQAQFAYPPTPE
                     TIRATFRAVKHCTARGVAINTFMLDSSYYLKAFMDEIAKINGGRVFYTTPEQLGEYIL
                     VD"
BASE COUNT         1046 a         1727 c         1661 g          806 t
ORIGIN      
        1 ctccgaccgg ctcgaatgcc tcatcttcgg gatgggcgac tactccgcca gccagcagat
       61 gcagctcggc cacatcggaa gctcgggagg ctacccgcca gacgtctggc actacccgcg
      121 ctacaagatg accatcgcct gccacgccaa cggtatcgac ccggtggacg gcccttacgc
      181 caactttcgc aactccgagg cctacggcaa ggaggccgag cgcgctttcg tgctcggcat
      241 ggtcggcaag tgggccatcc atccgagcca gatcgagctc gccaacgcgg ttttcacccc
      301 ggaccccgca gccctcgccg acgcccgcaa gcagaaagcg gcttacaacg aggccttgag
      361 ccaggggctc ggagccatca acgtggacgg cgtgatggtg gacgccgcca gtatccgaat
      421 cgtgcagaac ctgctcgatc gggcagaact gattgggatg tagccaatga ccgagactta
      481 cctcaccgac gagcgacgca tgattcagga ggtcgcccgg gacttcaccc ggcgagaagt
      541 gctgcccgtg gccaacgagc tcgaccccat ccagggggac atccccatgg acctgcgcga
      601 caagctcggc gagatgggct atttcggcat ccgcatggcc gaggagcacg gcggcctggg
      661 gctcggcgtg ttcgagtact gcctgatcag cgaggagctg gcgcggggct ggatgagcgt
      721 ggcgagcatc atcgcccggg ccacgtcgct catgaacgtg ggcggctggc cccttgagaa
      781 gcgcgaggag ctgacgcaga aggccgcgcg gggcgagtat ctggccgcga tctccctttc
      841 ggagccggac gtcggctccg atctggctgc ggccaagtgc cgggccgtgc tggatgggga
      901 cgagtgggtc atcaccggca gcaagtactg gtgcacgttc gccgacggtg cggactacat
      961 ccagcttctc gcccgggtcg aagcccccga ctcggacgat gcccagcaca aggggctgaa
     1021 gaacttcatc atcgagaaac cccgggggga gctgcccgag ggctgctccg gatcacccat
     1081 acccaagatc ggctacttcg ggtggaagac gtgggagctg gccttcgacg gctgccggat
     1141 ccccaaggaa aacatcatca ccggcggtgg cggtgacggt ttcaagaacg ccatgcactt
     1201 tctgaacgcc gcccgggccc acaccgccgc ccgctccatc gggctcgcgc gaggggcgct
     1261 cgaggacgcc atggcctact cccacgagcg ggtgcagttc ggcgtgccca tcgccgactt
     1321 tcaggaaacc cgcttcaaga tcgctcgcat ggccagcgag atcgaggcgg cccggcagct
     1381 gatgtaccac gctgccacgc tgctagatgc cggcgagacc gcggagcggg aatcggccat
     1441 ggtgaagtgg tacgccagcg agatggcaga gcgggtcacc tccgacgccc tgcagatcct
     1501 gggcggagcc ggctacacaa agctgcacgc ggtggagcgt cactggcgcg acgcgcgtct
     1561 cacgaagatc ttcgagggca ccagcgagat ccagctcagg atcatttccg atcggctgct
     1621 gggcaagccc agccaccgcg caaccgcctg agcgccccag cgggcggcgc ggcgccggcg
     1681 tgattgccaa gcgccagacg ggctgcgaga ctagatgcga cagacttgac gcggggcgct
     1741 gcaacgaatg ggggacaaag atcccggaga ccttccaacg acgttcggcg agctgaagtc
     1801 ctcgggctat caggtgctgc cggtaaagga agagctccgc cgtaacctga tcagccgcct
     1861 gcgcagccgc gagaccctgt ttccaggggt catggggtac gacgacacgg tcgtgcccca
     1921 gctcaccaac gccattctcg gcagccagga catcatcctg ctcggcgagc gcggccaggc
     1981 caaatcgcgc atggtgcgca gcctcaccag tcttctggac gagtggacgc ccgtgctcgc
     2041 gggaacggaa attccggaga acccctttca gcccatcacc gcccagggtc ggcagatcct
     2101 ggaagcccag ggggacagcg cccccattcg ctggctgcac cgggacgagc gatacgccga
     2161 gaagctcgcc actccggaca tcaccatcgc ggatctcatc ggcgaggtcg accccatcaa
     2221 ggtggccgaa ggccgctatc tctccgatga gctcaccctg cactacgggc tcgttccgcg
     2281 cgtcaaccgg ggcatattcg ccatcaacga gctgcccgac ctgtccgagc gcattcaggt
     2341 gggacttctg aatctcctcg aagagcgcga cgtgcagatc aggggtcaca aggtccggct
     2401 gccgctggac gtcttcatcg tggccaccgc gaaccccgag gactacacca accgcggccg
     2461 catcatcacc ccgctgaagg accgctacgg cgctcagatc cggacccact acccggcgga
     2521 cgtcgatcag gaagtggcca tcatggagca ggaagcagcc gtggccgaca tgggcgatta
     2581 ccggctcgaa atccccgcct tcatgaagga gatcgtcgcg gagatcaccc atcacgcccg
     2641 gcgctcgccc gacatcaacc agcgatcggg cgtttccgtg cgaacgtcca tcgccgatta
     2701 cgaagcgatg ctggcgaacg ccttccggcg ggcgctgcgc ctcggcgagg acgaggtggt
     2761 gccgcgcatc tccgatctgg cgttcgtgct cccgtcgttt cagggcaagg tcgagttcga
     2821 ggccatggag gaaggccagg aggacaagat cacccagcgg atcatgcagg ccgccatcaa
     2881 gtcggttttc gatcgctact acgacgtgaa cgatctggag tccattgccc tggctttcaa
     2941 cgacgggctc acggtggata ccggcgaggc cacacccgca gcggactaca acgccatcgg
     3001 cgagcgcata cccggcctga tggacgtgtt tcccgacagg gagggcgtaa gccccggcgt
     3061 gcgggcggcc atcatcgagt tcatcctgga ggggctgcac ctgcacaagc ttctcaacaa
     3121 gttcgacaac aggggagctg ccacctactc ggggtgagcg gcaacccaca ccacagctga
     3181 ggccgtcatg cgtcactttc cctcaggcta ccgattctcc cgctgggacg gctcccagaa
     3241 gctgccgctg gacgccgacg agatcatggc cgcgctggcc gacgacctga tggaatacgg
     3301 cgaccttcgc tgggccatgc gcaacctcat gtcacgcggc atgcagatac ctcagggcgg
     3361 ctacatgcag ggcttgcggg acatgctccg gcagctgagg gaacgcaagc gggagcagct
     3421 cgagcgcttc gacctgtcga gcgtgttcga ggacttccgg gagcggctcg acgagatcct
     3481 cgccatggag cgaaagcgca tcgacgagtg gctcgatccg aagcccgcaa cggattccga
     3541 agctcccgct ggcaacgggc cgagcggcga acccgagcag agcacgtctg ccgaccagcc
     3601 tgccgcccag ggagacggtc ccgatttctc cggcgacgtg ctcaagcaga tcgccaagcg
     3661 caaccgggac gagctcgacg ggctgccgcc ggatctcgcc gggcaggtcc gaaccctgga
     3721 gaagtacgag tttctgaacc ctgacgccca gcgaaagttc ctggagctgc tcaacgagct
     3781 gcgccgggcc atgacgaaca cgttcttcaa cgacatcgag aacatggtca accagctctc
     3841 cgacggcgac gtcgagcgca tgaaggagat gatcaaagca ttgaacgaga tgctggtgaa
     3901 gcggatcgcc ggggaggatc cgggattcga ggatttcatg gcccagtacg gggacatgtt
     3961 cggtgacgat ccgccccagt cccttgacga tcttctggag cgcatggccc agcagatggc
     4021 ggccgctcaa tccctgctga actccatgtc gcccgagcag cagcaacagc tgcagtccct
     4081 gctcagtgat cgcttcggcg atccggagct gaactctgag cttcgcaagc tcgccaagga
     4141 gatggacttc ctcaacccgg acggcggcaa gtaccggttc tccggagacg agcggctcga
     4201 tctcgaaacg gccatgaagc tgatgagcga gatgcaggac ctcgatgagc tgatctccca
     4261 ggtccaggaa gcagagcgcg gtgggggcac ggaccgtatc gatcgggacc tgttgagcga
     4321 cctgctcggg gacgcagcgg ccgacgacct cgagcagctg cagcagatgc tcaaggccct
     4381 ggaggaagcg ggctacatcc gtcccaccga cgacgacggc tgggagatca cgccgagggg
     4441 ttcccgcatg atcggccaga aggcccttgg cgagatctac gccaggttga agcggcagaa
     4501 cctcggcaat catgccgtgc ccgaggaggg gcgtttcggc gagcggctgg agcagaccaa
     4561 agcctacgag ttcggcgacc cgttccatct gcacatgccc cgaaccctgc gcaacgccat
     4621 cgatcgggag ggcccgcaga cgccggtgag cctcaagccc gaggacttcg agatctaccg
     4681 cagcgagctc atcacgtcca cggccacggc catgctggtg gacctgtcct ggtccatggc
     4741 gctcaggggc tctttccagg cggcaaaaaa ggtggcgctg gcgctgcaca acctcatcac
     4801 ctcgcaatat cccaaggaca ccttctacat catcggcttt gccgcttacg ccaaggagct
     4861 gaagccgaag gatctaccct accttcagtg ggacgagtac gtgctgggga ccaacatgca
     4921 gcacgccctg ctgctcgcgg agcgtctgtt ggccaagcac caggcaggca gcaagcagat
     4981 catcatgatt tctgacgggg aacccaccgc ccatctggag aacggccagg cccagttcgc
     5041 ctacccgccc acacccgaga ccattcgcgc cacctttcgc gccgtgaagc actgcaccgc
     5101 gcggggcgtg gcgatcaaca cgttcatgct ggattcgagc tactacctca aagccttcat
     5161 ggacgagatc gccaagatca acggcggccg cgttttctac acgacgcccg agcagctcgg
     5221 cgagtacatc ctcgtcgact
//
LOCUS       DBNHLM010000041        35441 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_245109, whole genome shotgun sequence.
ACCESSION   DBNHLM010000041 DBNHLM010000000
VERSION     DBNHLM010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 35441)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 35441)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35441
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_245109"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="AC16QF_01890"
     CDS             complement(<1..261)
                     /locus_tag="AC16QF_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447332.1"
                     /translation="MTSRPSSRRRAGCCLLLAGLLTGAAAAEDAAPVTGVAERYWTPR
                     DELPERVAARLPSYCDGAYLQPAFPYPRSVDDEDYPLHAEARL"
     gene            325..1332
                     /locus_tag="AC16QF_01895"
     CDS             325..1332
                     /locus_tag="AC16QF_01895"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR6447333.1"
                     /translation="MESRSIPTELAQWLAGELARLDLDPTFTSQALLVEASYRCFYRI
                     RSRHRGGEALSLVAMESPPERENNQQFVTLAKVFAAHGVGVPRILAADLTDGFLLMTD
                     LGDRHFADAYQSGERDRALAAGIDTLIRLQTVDDPAVPLYTARRFRDELGIFREWFVE
                     RWLGEPFPAEDLAGIPERLVACVEQQLQVCVHRDFHSRNLLLAADGSVGVVDFQDALI
                     GPVSYDLASLLRDCYYRFPEDEIARWRDYYLERSPHALDPEQFARDLDLTAAQRQLKA
                     VGIFARLDLRDGKTSHLRHIPPVLDHLAALSARHDTLTPLGPHLAVWQKAAERRLQAR
                     R"
     gene            1329..2000
                     /locus_tag="AC16QF_01900"
     CDS             1329..2000
                     /locus_tag="AC16QF_01900"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR6447334.1"
                     /translation="MRAMILAAGRGERLKPLTDAVPKPMLTVAGEPLIARQLRALGEA
                     GITRVVINLHHLGEQIASFCGSGKDYGVAIDYSREDELLDTGGGIVKALPLLGDAPFL
                     ILNGDILTDFPFQTLATALPDWADLHLVLTSRPAHRAHGDFDWEAGRVTGRGESYVYC
                     GIAVLRPELFDGRIIEPFSLQQILFDAVAGGRVSAEIWTGTWIDIGSHDQLRAANEHL
                     KSEPG"
     gene            2012..2704
                     /gene="rpe"
                     /locus_tag="AC16QF_01905"
     CDS             2012..2704
                     /gene="rpe"
                     /locus_tag="AC16QF_01905"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR6447335.1"
                     /translation="MSYWIAPSILSADFSRLGAEIEAVLGAGADIIHFDVMDNHYVPN
                     LTIGPLVLQSLRKAGIDCTMDVHLMVKPVDRLVGDFIEAGADIISVHPEATEHLHRTL
                     SLIRDGGARAGLVFNPATPLGVLEEVLDLVDLVLVMSVNPGFGGQAFIASSLDKLAAA
                     RRLIDGSDRAIRLEIDGGIKADNIALAAQAGADTFVAGSAIFGSPDYAATIASMRAAL
                     EGVQPGTPRGAA"
     gene            2701..3345
                     /gene="gph"
                     /locus_tag="AC16QF_01910"
     CDS             2701..3345
                     /gene="gph"
                     /locus_tag="AC16QF_01910"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01449.2"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HIR6447336.1"
                     /translation="MKPYTGYLFDLDGTLIDTAPDINAALNHALTQCGAPTVNEALTR
                     HWVGHGARVLVEQALAYHELPEEDLAPIHEAFLAYYGDHAAEHSRVYPGVVDTLTALK
                     ERGARLAVLTNKLTRFSLPILSTLGIDGYFDATICGDTAAAPKPAADPALLACRELRT
                     TPGDSLVVGDSQTDVLCARAAGCPVVCVRDGYNHGVPAEQLGADAVIESFLDLL"
     gene            3356..4840
                     /gene="trpE"
                     /locus_tag="AC16QF_01915"
     CDS             3356..4840
                     /gene="trpE"
                     /locus_tag="AC16QF_01915"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIR6447337.1"
                     /translation="MNQAQFAELAEAGYNRIPVVFETLADLDTPLSTYLKLARGPYSY
                     LLESAHGGEKWGRYSIIGLPCQTVLKVQGNQITVETDGELVETHESPDPLSFIEEFQA
                     RYRVAALPELPRFYGGLVGYFGYDTVRYAERRLADGAPPDPLGTPDIVLMVSEDLVVF
                     DNLRGRMQLISLVDPAEPDVYQRTMERLRDRALALSEPVPPIPESATNQPVLESDFAS
                     EFGEEPFKEAVEKIREYIRAGDVMQVVLAQRMSLPFGADAIDLYRALRSLNPSPYMYF
                     MDLEEFQIVSSSPEILARLEDGKIINRPLAGTRRRGHTPEEDLALEQELLSDPKELAE
                     HLMLIDLGRNDVGRVSEIGSVEVTEQFVIERYSHVMHISSNVVGRLAEGKTAIDVLRA
                     TLPVGTLSGAPKIRAMEIIDELEPVKRGIYGGAVGYLAWNGNMDTAIAIRTAVIKDGT
                     LYIEAGGGVVADSVPRLEWKESMNKGRAIFRAAAMAENRFRDLG"
     gene            complement(4932..7370)
                     /locus_tag="AC16QF_01920"
     CDS             complement(4932..7370)
                     /locus_tag="AC16QF_01920"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6447338.1"
                     /translation="MTTYLRALNYPRTLLTLVALLLLVAGVCGTRFSFDASSDTLVVE
                     GDPDLATYLRIAETFGGDEFLLLTFTPRDGDALDPANLATLRALQQEIAALEGVGSVF
                     SILDAPLLKSPPVPLAELARGFRTLESADVDRPSAREELKESPVFGGLLITADARTTA
                     MRVDISLDRELLVLDEERARLRSQAERSAAEVKRLAELEVARETLREAYVNGRERLIG
                     QIRDVRDRYADRGVLHLGGVPMIAADMIAFVKSDLVVFGGSVVVIVMVTLYGFFRRLR
                     WVLLPVVTSAVTIGFTVGVLGLAQWPATVISSNFVSLLAIITISLTIHLVVRYRELAH
                     LHPDMAADELVRQTMVSKFAPCLYNALTTIAAFGSLTASQIVPVEDFGWMMCIGIAIG
                     FFTSFTLFPALLLLLGRVAGPSLLSRELGVTRVFSHLARWRAPLVVVISAVWAAFAAL
                     GVSMVSMDNRFLEYFRENTDINQGMAFVDAQLGGTVPFDVVLEFPPFENLVEEEDDFF
                     SEEETYPERYWFTRDKLDKLMLVHRYVEARPEVGKVLSLTSLEDLARQFTEGDALSNL
                     EVAAVLDVLPEHLRDELIRPYASPSTGELRINARVIESGRSFDRQALAADIRDFAVTQ
                     GAVPADAVQVTGMMVLFDSMLKQLLSSQLDTLVYVLLATFAMFVILLRSFLYALLGLI
                     PNVIAAASVIALMGYVGIPLDMMTITIAAISIGIGVDDAIHYLHRFDEERRRREDVRM
                     AVAWSHATIGRAMYFTSVTVIIGFSVLVFSNFVPTIFFGLLTAAAMALALIANLTLLP
                     SLLVLVIGRREASA"
     gene            7442..7966
                     /locus_tag="AC16QF_01925"
     CDS             7442..7966
                     /locus_tag="AC16QF_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIR6447339.1"
                     /translation="MVENIIQLIEEVAEALLENRSRLITAESCTGGMVAAELTSRPGS
                     SEWFEGALVTYRLSAKQRLLGVPAAMLDRYGPVSEPVARAMAEGALRVSDADVSVAIT
                     GIAGPDGGDMLAPVGTVWFAWARRDEPGAEPVCLQATQHRFSGSRDEVRTQAVALALE
                     GVLASLSGINPSRS"
     gene            complement(7947..8423)
                     /locus_tag="AC16QF_01930"
     CDS             complement(7947..8423)
                     /locus_tag="AC16QF_01930"
                     /inference="COORDINATES: protein motif:HMM:NF037143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR6447340.1"
                     /translation="MDLNQIKADVGQTWKWLADGWRSLSDKAVNALTYFTPGRNEPAD
                     PDEIRWGLLAADVSESGEHVTVELEAPGLEKDEIDVSVDERQVTITGQKRVESERREG
                     AMVVRERAFGRFQRTIPMPVAVTADGAEASYRRGVLRIRVPKVASPGGRSIKIEKG"
     gene            complement(8433..8846)
                     /locus_tag="AC16QF_01935"
     CDS             complement(8433..8846)
                     /locus_tag="AC16QF_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR6447341.1"
                     /translation="MSEPVRSGDPFDLFSRMMGGTDREPHAGSRDWLPAVDIREEQEG
                     YLLVMDVPGLGAEDLDVDLHEGVLTVRGARSAEETKQGEHYVRVERRHGKFARQFRVP
                     PSARPEDLRANVKDGVLTIHVPKGGAPETRRVPVS"
     gene            complement(8930..9424)
                     /locus_tag="AC16QF_01940"
     CDS             complement(8930..9424)
                     /locus_tag="AC16QF_01940"
                     /inference="COORDINATES: protein motif:HMM:NF039797.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HIR6447342.1"
                     /translation="MELTMNGMAKGLAAALTAGALLTGCASDPAETAAAQQEVSEQID
                     TILTQPLGEEDYGGGERCISTNLYDSVDVLDTRHVLFKGRGNRLWVNTLRSTCVGLRK
                     RDVLQFEIRGSQICNLDTFTAVSNSFGWQPTSATCSLGDFSPVTAEQVAMIEAALDTK
                     KKKK"
     gene            9572..10147
                     /locus_tag="AC16QF_01945"
     CDS             9572..10147
                     /locus_tag="AC16QF_01945"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019472944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR6447343.1"
                     /translation="MLLMIDNYDSFTYNVVQYLSELGAEVAVYRNDEITLTEIEELAP
                     EKIVISPGPCTPDEAGVSMEVVTRFGPTIPILGICLGHQSIGQAMGGTVRRAREVMHG
                     KTSLIHHDDRGVFHGLPRPFEATRYHSLVVAHEDLPDCLEVTAWTERDGELDEIMGLK
                     HREYPIEGVQFHPESIRTPVGHDLLRNFLEG"
     gene            10153..11175
                     /gene="trpD"
                     /locus_tag="AC16QF_01950"
     CDS             10153..11175
                     /gene="trpD"
                     /locus_tag="AC16QF_01950"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR6447344.1"
                     /translation="MDIQGALAALFEKRDLSLEDARAVFVEVMSGHATPGQIGALLAA
                     LRVKGETPQEIAGAAEAMRSLSTKVVVDVPYLVDTCGTGGSGAKLFNVSTAATFVAAA
                     AGAHVAKHGNRKMTSFSGSADVLEAAGVNISLAPEQIAHCIREVGVGFLFAQAHHSAM
                     RHAAPVRQELGARTIMNVLGPLTNPAGARNQVLGVFSAAWQTTLAEVLKLLGASHVLV
                     VHSNGLDELALNGPSFVVELNAGTITEYEIEPADFGLKPSSHDELRADSPETSLALLK
                     RSLTEPDSSAADIVSLNAGAAIYAAGVATSFKNGVVMAQDAIAAGLASERLAELVRVS
                     SMMAEP"
     gene            11223..12008
                     /gene="trpC"
                     /locus_tag="AC16QF_01955"
     CDS             11223..12008
                     /gene="trpC"
                     /locus_tag="AC16QF_01955"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR6447345.1"
                     /translation="MTILDEILAHKRTEVAERQRVRSLDSLRALAQACPPARGFADAL
                     ESKLLAGASGVIAEIKRASPSKGVIREDFDPVSIARSYAKGGAACLSVLTDERYFQGH
                     DEYLQAARAAVDLPVLRKDFVVDAYQLFEARAMGADCVLLIAAALDAGQLKALHETAI
                     ALGLDVLVEVHDGEELESALEIEPRLVGINNRNLKTFETRLETTYGLLPGIPDSVTVV
                     TESGIANREDVAAMRQHGVHCFLVGEAFMRAPDPGTALAEMFA"
     gene            complement(12153..12812)
                     /gene="crp"
                     /locus_tag="AC16QF_01960"
     CDS             complement(12153..12812)
                     /gene="crp"
                     /locus_tag="AC16QF_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HIR6447346.1"
                     /translation="MGKPLTAKGFNPNAGQEDTHLDAFLAAAHRRKYPAKSTLIYAGD
                     KSDSIYYILKGSVTVLVEDESGREIIVAYLNKGDFFGEMPLFTESAPRSAWIKAKTEC
                     EVAELSYGKFRELAQRHSDLLYAVGRQMADRLGRTTQKVSDLAFLDVTGRVARTLIEL
                     TKQPDAMTHPDGMQIKITRQEIGRIVGCSREMVGRVLKTLVEQGLVNVKGKTMVVYGT
                     R"
     gene            13012..13431
                     /locus_tag="AC16QF_01965"
     CDS             13012..13431
                     /locus_tag="AC16QF_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014872825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR6447347.1"
                     /translation="MQAKITLIENVHFEAVADSGHTVLIDGPPDAGGENRGTRPMELV
                     LMGLGGCASFDVVSILRKSRQPVVGCVTRLTARRADTVPQVFEAIHLHFTISGQGLDE
                     AKVARAVALSAEKYCSASIMLERGGVAISHDYEILEA"
     gene            13451..13864
                     /locus_tag="AC16QF_01970"
     CDS             13451..13864
                     /locus_tag="AC16QF_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012312660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6447348.1"
                     /translation="MTKPPKPHAGLRHVALFTTTLEASVRFYTELLGMQVEWNPDPDN
                     FYLTSGTDNLALHRATEGRSVRGQTLDHIGFIIDDEEDVDAWHDHLAANGVNILERPR
                     THRDGARSFYCEDPDGNVVQLIYHPPISRPGGQTR"
     gene            complement(13852..14490)
                     /gene="coq7"
                     /locus_tag="AC16QF_01975"
     CDS             complement(13852..14490)
                     /gene="coq7"
                     /locus_tag="AC16QF_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HIR6447349.1"
                     /translation="MAGPGSLADALVAQVDKALRTLTGAAASVRENPAADVPEGEMSP
                     GEREHAAGLMRVNHTGEICAQALYEGQALTASDPRTRTALLAAASEESDHLAWCAGRL
                     AELGSSPSVLNPAFYALSYALGAVTGVLGDKVSLGFVEATEDQVCRHLESHLQSLPAG
                     DSRSRQIVRAMREDEARHGAEALGAGGAEFPQPVKRAMSLASRLMTETTYRV"
     gene            complement(14492..14836)
                     /locus_tag="AC16QF_01980"
     CDS             complement(14492..14836)
                     /locus_tag="AC16QF_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR6447350.1"
                     /translation="MSSDCLFCRIASGEIPGDIVYEDDELLAFNDISPQAPHHVLVIP
                     KIHVATVNDLAAEHADLVGRLVLCARAIAAERNIQESGYRLILNCNGHGGQTVFHLHL
                     HLLGGRQLMALG"
     gene            complement(14911..15708)
                     /locus_tag="AC16QF_01985"
     CDS             complement(14911..15708)
                     /locus_tag="AC16QF_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR6447351.1"
                     /translation="MREPDQRVAAGSPRSRWPIYPGAQGVLTLSPVTLVRVSEDAAAA
                     TAHAREVQPPLSRPQWLTDGWECRFLSSGAMGRVSLDHCQRVMRAMRPVDLLRGEMVV
                     RTGEPGDTVFVLAAGRAEVSRGRRSLALLEPGDHFGEDAAISGACRNACVTMVSDGRV
                     MTLSGELFRDFVVGRVLLDDGDGPAQATIDIGGRSVSAGLGIPLLLLRERLGLLTPEL
                     RYRLAGGSLRQRVLAAFILLHRGFRVALAESGLDAEVALHPEPRAHL"
     gene            complement(15951..17231)
                     /gene="purD"
                     /locus_tag="AC16QF_01990"
     CDS             complement(15951..17231)
                     /gene="purD"
                     /locus_tag="AC16QF_01990"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009041976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR6447352.1"
                     /translation="MNVLVIGSGGREHALAWKAAQSDGVETVFVAPGNAGTATEAGCE
                     NVDIAVDDFPALAEFAAANGVGLTIVGPEVPLVEGVTDYFEGRGLACFGPSKMAAQLE
                     GSKAFTKAFLDRHRIPTARYQAFTDLAAAQAYVREQGVPIVIKADGLAAGKGVVVAET
                     IEQALQALDDMLSGSAFGEAGSRVVIEEFLAGEEASFICISDGTNVVPFATSQDHKAR
                     DDGDKGPNTGGMGAYSPAPVVTAGVYDRIMKEVVNPTVRGMAEDGVPYVGFLYAGLMI
                     MPDGAPRVIEFNCRFGDPEAQPVMMRLRSDLVGLCRQALDGELAGVELDWDPRVALGV
                     VMAAGGYPEAYDKGDAITGIDTADADPRVKTFHAGTRLEDGVLVTNGGRVLCVVGLGD
                     DVAGARAAAYGAVEHIDWRNRYYRRDIGHRALDR"
     gene            complement(17268..18824)
                     /gene="purH"
                     /locus_tag="AC16QF_01995"
     CDS             complement(17268..18824)
                     /gene="purH"
                     /locus_tag="AC16QF_01995"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR6447353.1"
                     /translation="MSNADARSVARALLSVSDKTGLEEFARGLSALGVEILSTGGTYR
                     ALSEAGIRVTEVSAHTGFPEMMDGRVKTLHPKIHGGILGRRDVDGATMTEHDIEPIDL
                     VVVNLYPFEATIERADCTDELAIENIDIGGPAMVRSAAKNHRDVLVVVDPGDYGHLLE
                     TLQRDGATTLESRRALAAKAFRHTARYDALVAGYLTAEQEFPESLTLSYRHVETMRYG
                     ENPHQQAAFYRESGARPGTVAALRQLQGKSLSYNNVADTDAALECVKVFERPACVIVK
                     HANPCGVAEAADVQAAYERAFATDPTSAFGGIIAFNRPLDEPTLTLILERQFVEVIVA
                     PEAAPEAVAAAAARKNVRLLVTGPLQAGSRGLELKRVGGGLLVQTPDETCLGEEGLRV
                     VSRRAPSASELDDLLFAWKVAWFVKSNAIVYARDGQTIGVGAGQMSRVVSARIAGFKA
                     AEEGLEVAGSVMASDAFFPFRDGIDAAAEAGIAAVVQPGGSVRDDEVIAAADEHGMAM
                     VFTGIRHFRH"
     gene            complement(18821..18919)
                     /locus_tag="AC16QF_02000"
     CDS             complement(18821..18919)
                     /locus_tag="AC16QF_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6447354.1"
                     /translation="MARIGGNKSRAATMLGMNRGTLRKKLEGYGII"
     gene            complement(18919..19908)
                     /gene="dusB"
                     /locus_tag="AC16QF_02005"
     CDS             complement(18919..19908)
                     /gene="dusB"
                     /locus_tag="AC16QF_02005"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR6447355.1"
                     /translation="MTVTAIGPFKLRNDVLLAPMAGITDAPFRALAWRFGAGYVVGEM
                     VGSREALWHTPKSRLRRCRVDGVEPFAVQIAGADPRDVAESAARHVEEGAQVIDINFG
                     CPVKKVCNKAAGSALLKEPDLVVAIVRAAVAAVPVPISVKMRTGWSPDSRNAVELAQR
                     MEDEGVAAIAVHGRTRACRFNGEAEHGTVAAIKSRVSIPVFANGDIDSPGSARRILEE
                     TGVDGVMIGRAALGRPWLLGEVADGVPRVASLGEKLAVVAEHVAALHEFYGEPGTRIA
                     RKHVQWYLQHLSQLGPREVRSRLGKAFNRLEQAQAQLDFLAELPASAIGRRAA"
     gene            complement(19939..20856)
                     /locus_tag="AC16QF_02010"
     CDS             complement(19939..20856)
                     /locus_tag="AC16QF_02010"
                     /inference="COORDINATES: protein motif:HMM:NF023334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HIR6447356.1"
                     /translation="MIEQKLESMITECPHCQTRFRVTDVQLEAAAGRVRCGSCLNVFQ
                     GARHMVPARVPEPAPRRTATVRHLYGGHEAQPPSAEPDESSQVTGARDAAGPPPPPEA
                     DHAARAAEPESADTPSAAGQGAALEVPRSLKKLSAAEAQGLALYAQPPRRRWWAPLAG
                     AAAVLLVATQVLWLQFDTWARDPQVRPLYAMVCGVVGCELPVMRDVESLLAKNLVVRR
                     DPERTDALLVNAIIVNQAAFAQPFPVLELSFTALNGSPVKEERFEPGTYLAGEMAGAT
                     DMPPRTPVHIELTVAHPGDMAVNYSLSFR"
     gene            complement(20905..21768)
                     /gene="prmA"
                     /locus_tag="AC16QF_02015"
     CDS             complement(20905..21768)
                     /gene="prmA"
                     /locus_tag="AC16QF_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR6447357.1"
                     /translation="MSWLKFELDVEIDAADALSDRLLELGAVSVSLAPLSDELLLEPA
                     PGETPLWRRSRLSALFSADADVEALREFLAAHGDVDEGFVEDQDWVDAWRSSAVAECF
                     GGRLWVVPRDSEARNGTVLRLDPGLAFGTGGHPTTRLCLGWLASHDLAGQRLVDYGCG
                     SGILGIAAALLGAAQVLAVDHDPQALLATEENARANAVDARRLRAVGPDEVPGRGDYD
                     VLVANILANPLLELASRFTDLIRPGGMIVLSGLLARQASEVVAGYPSFRFADPQPDGD
                     WVLLSGRKRDR"
     gene            complement(21773..22171)
                     /locus_tag="AC16QF_02020"
     CDS             complement(21773..22171)
                     /locus_tag="AC16QF_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010795424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR6447358.1"
                     /translation="MDLRKIKKLIELVEESGIAELEVRDGEEAIRIARPVAGTVPAPV
                     AQVRAAQPGAVAGTITAPMAGTFYAASEPGAEPYLKVGQQVEVGDVVCIIESMKMMNE
                     IVADQSGTCVSVAVGNGQAVASGQVLFNIA"
     gene            complement(22184..22615)
                     /gene="aroQ"
                     /locus_tag="AC16QF_02025"
     CDS             complement(22184..22615)
                     /gene="aroQ"
                     /locus_tag="AC16QF_02025"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR6447359.1"
                     /translation="MARFLVLHGPNLNLLGTREPEIYGTDTLEDINSRLEALAREAGN
                     EATCLQSNAEHELVDAVHGAKTQGVEGIVINPGAFTHTSVALRDALLGVGIPFIEVHL
                     SNVHAREEFRRQSYLSDVARGVITGLGAKGYEFALLAAMAR"
     gene            complement(22703..24613)
                     /gene="dsbD"
                     /locus_tag="AC16QF_02030"
     CDS             complement(22703..24613)
                     /gene="dsbD"
                     /locus_tag="AC16QF_02030"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     [NAD(P)H] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HIR6447360.1"
                     /translation="MRRFWPGLGAAAIGFTLLLAAPGAAASALDDISTGAAGWLSEQP
                     TFLPVDEAFAMSAEVGPDGAILVRWDIADGYYLYRHRFTFEAREPAGGGAIAVLGAPE
                     IPPGKAKVDEYFGEVEVYYGRATARLPVQGGATVEVGIGYQGCADLGLCYPPETRWVT
                     LTMPGAPGAISGGDAGSAPGSPAATVQTEERALAGLLAEGSLLTALLLFFVAGVGLTF
                     TPCVLPMVPILSSIIVGDSEKLTRSRGITLSLAYVLGMAFTYAIIGTLMGMFGASLNM
                     QAALQSPPVLVGFALVFAALSLSMFGLYELRLPASWQSAIDAVGSRTGGGKHLSVAVM
                     GSLSSLVVSPCVSAPLAGALIYLSATGDAVLGGGALLALGLGMGVPLLLIGASGGHML
                     PRAGEWMNAVKILFGVLLLAVAVWLLERVVPGPVNLALWAALAIGAGGYLGLRAWSPR
                     GMVSGAVRAAGLLGVVYGALLLVGAAGGGNDPLRPLAGLTAWDPAHQPGEALEWQPVD
                     DVAGLRAAVLAASREGKPVMLDLYADWCISCKVLERSVFPKPEVAARLSRFNLLRADV
                     TANDDHHKALLDELGVFGPPSMVFFAEDGRKLAEVTVQGEIGADALAAHLGAVLERVG
                     SNNFSEIAVNFR"
     gene            24827..25447
                     /locus_tag="AC16QF_02035"
     CDS             24827..25447
                     /locus_tag="AC16QF_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447361.1"
                     /translation="MPEGARADPLTEPAPAPAPHAAEIDALLLQANDAMERGQYINPA
                     RGSALGLYDRVLALNPGNAEARRGLELIAQHYLDLASAAVRRRQLTRARSMVDWARIV
                     DAEHPGIAAAEAQIRLLAEARRVRLPVAGSQLRDRSDALARSLAELGSRARNGNCLAI
                     IRSPSDASGRWIYQQMSRAPGTARIRADIEIGSPPMVELLCFENDA"
     gene            25447..25905
                     /locus_tag="AC16QF_02040"
     CDS             25447..25905
                     /locus_tag="AC16QF_02040"
                     /inference="COORDINATES: protein motif:HMM:NF016221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HIR6447362.1"
                     /translation="MPDAAILAAALAAALAAAPVAIPASSAPALAGSELVVTDARVRA
                     LLPGQRHTVAYFAIENRGPASVTLVSAESAAAGAMEIHESRRDGDMYRMRRRAEVEIP
                     ARTTVRFDPGGLHLMLFDVQALGEQMEIELRTSDGRRLKAIFNRIAAGAE"
     gene            25902..26948
                     /locus_tag="AC16QF_02045"
     CDS             25902..26948
                     /locus_tag="AC16QF_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009041985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2333 family protein"
                     /protein_id="HIR6447363.1"
                     /translation="MKINWRFWASSNDADVTDGRLATAGRIGLGAVAAYLLVCVLLGW
                     WWDKEPDVFAVRENASSRAELGNEPLVTGYITTATLIRLGETLLDKRGGYLSNDVFPP
                     GVWMDNIPNWEFGVLTHIRDATRTYRNELSRSQSQSAEDPDIVRAEGKFFIDNASWFI
                     PQAESEYRDGIKALSSYLQRLADPTQPDAQFYARADNLQQWLQIVEARLGSLSQRLSA
                     SVGKRQLDVSLAGDAAASQATESPREQEVKTPWLEIDDVFYEARGQTWALLHLLRAME
                     QDFGDVLDKKNARVSLQQIIRELEPTQDTVWSPIILNGDGLGLLANHSLVMASYVSRA
                     NAAIIDLRALLSQG"
     gene            27131..29158
                     /locus_tag="AC16QF_02050"
     CDS             27131..29158
                     /locus_tag="AC16QF_02050"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIR6447364.1"
                     /translation="MNNIALIPPIFGIVGLIVAFFIFSLVKRYDEGTEEVKRIGDAIH
                     EGAMVFMRREYTMLAGFAAALIVILYIALGTETTIAFIVGALASATAGWIGMYTATKA
                     NVRTTTAAHTAGAAQALSVAFFGGSIMGLTVASMGLLGLGILYYVFGGDPETAHHIHG
                     FGMGASTVALFSRVGGGIYTKSADVGADLVGKVEAGIPEDDPRNPGVIADNVGDNVGD
                     VAGMGSDIFESYCGSMIATIAIASTLALAALTDLAPGMDEGSARAALMGLPLILASTG
                     LICSLVGIALVRLMSDKEPAVALRFGTLGAPVIFIIAAWFVIGQVGLESAVWWSVVSG
                     SVGGIIIGLVTEYYTSSKPIIRIAEAGETGAATVMITGLAVGMQSVVVPILAICGIIF
                     VSTHLAGLYGVGIAALGMLGTVGITMAIDAYGPVADNAGGIAEMSGLGKETRAITDSL
                     DELGNTTAAIGKGFAIGAAALAALAIIAAYVETISYQDPSFTLHIGDPEVLIGLFIGG
                     LIPFLIASITMTAVGDAAMEMIQEIRRQFREIPGLLEGKADPDNAKCIDIATQAALKR
                     MLLPGVIAVAVPPVVGFGLGPAALGGTLGGGLLGCVLLALTMANAGGAWDNAKKYVEK
                     GNLGGKGSDVHAATVVGDTVGDPFKDTSGPSMNILINVMAIVSLVIAPLLV"
     gene            complement(29210..30472)
                     /locus_tag="AC16QF_02055"
     CDS             complement(29210..30472)
                     /locus_tag="AC16QF_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR6447365.1"
                     /translation="MKRANAFYAQSGGVTAVINASACGVIQTARKHRDRIGKVLAGRN
                     GIIGALAEELIDTGRESAKDIAGLRTTPSGAFGSCRYKLKSLEENEREYRRLIDVFRA
                     HDIGYFFYNGGGDSQDTAHKVSQLGGRLGYPITCIGIPKTVDNDLPFTDCCPGFGSVA
                     KYVAVSIREAAMDVASMCETSTKVFILEVMGRHAGWIAAAGALAQEGPRDAPHIILLP
                     EVPFDRKRFLAKVRETVDGCGYCVIVVSEGAQYPDGTFLADAGSKDAFGHTQLGGVAP
                     TLAAMIKDELGYKYHWAVADYLQRAARHVASATDVEQAYAMGRAAVELALAGENAVMP
                     IVVRKSDSPYRWTVGSVKLSRVANVERKMPKSYISKDGFGITARGRRYLAPLIAGEDY
                     PAYRNGLPQYVRLKNELVAKKLEPFQVS"
     gene            30557..31045
                     /locus_tag="AC16QF_02060"
     CDS             30557..31045
                     /locus_tag="AC16QF_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447366.1"
                     /translation="MQLFWQICRLKQSPEYVPTQTWFVLLVIAANLICSAAVSLSAEG
                     APGLLTTINSIVVGQTTTAALVWVALNLRDMGGRFLATITALFGCDLIITAVFALILP
                     LTLLAPTMAPVLFLLFVAWSVSVAGFILHRALQVPLALGIVVALGMSVLSLALSQLAI
                     GA"
     gene            31042..32445
                     /gene="mpl"
                     /locus_tag="AC16QF_02065"
     CDS             31042..32445
                     /gene="mpl"
                     /locus_tag="AC16QF_02065"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HIR6447367.1"
                     /translation="MNAERRIYFLGIGGTLMGSLAQLAREMGYRVSGSDVAIYPPMSD
                     QLAAADIEVFEGFDPAHLDPEPELVVIGNAGLPRGHPGVEHIIENGLRYVSGAEWLGS
                     QVMPGRWVLAVTGTHGKTTTASMLAWILDQAGLKPGYLIGGVPKNFPRSSRLGDDPFF
                     VVEADEYDCSYFDRRSKFVHYHPRTLIINNLEYDHADIFPNLAAIQTQFNHLLRTVPG
                     HGLVIAPSNDDNVNETLQQGCWTPIARVGAPGVKQPLERDNGERWSASRGDGTGAEFD
                     VCLGEENLGTVRWPIFGDFNVRNALAAIAAARHAGVPPEVAIDALGSFEGVKRRLELI
                     AEIGATLIYDDFAHHPTAIRATLQGLRNKVGSEEIIAVIEPRSHTMSLGTLRNELATC
                     CSPADEVYWFRGENIQWDLSELLKSCVIPAHINFELDKLIEALAQLPQKRRHIVIMSN
                     GSFGGIYQKLPTRLRSG"
     gene            32490..33425
                     /locus_tag="AC16QF_02070"
     CDS             32490..33425
                     /locus_tag="AC16QF_02070"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR6447368.1"
                     /translation="MQSRPACFVAALLLAFVPAANAADWYPSRYGADDTLGAINNLSP
                     EKVVQAARLVTEGKTYPLGVETGPDSPAYPPRSYSLTVLQPGDGTGSLMGTNRASGND
                     DLLSFWMGIGSQIDGLGHMGIEHVYYNGNRAQDFVSATGLSRLSIDRLPPIVGRGVLI
                     DMAAHMGKAILPAGTAFNSREIKAAAKAQGVTIGTGDVVLFHTGWLNVADDKARFMAG
                     EPGLGVDGARYLAGLGVVAVGSDTWGLEVLPSEVEGEAFPVHPELLAKNGVYILENMD
                     TRALAADGVHEFLFVLGQPRFVGAVQAVINPVAIK"
     gene            33425..34108
                     /locus_tag="AC16QF_02075"
     CDS             33425..34108
                     /locus_tag="AC16QF_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447369.1"
                     /translation="MKAPRRPTAQAAIRAAAVAGLLLAGCDTGSGAEDTAQAQARTGA
                     EPASPELLMARPPEGWKVWSATQDPGLRVAQYVPADTDADNWEQMLRLESMTGDPLPD
                     PIEFLTALGRDQAAGCGQSSDLNIHSGQENNYATSVRLFSCRNDAAPDLDQVTLIKAI
                     RGNENFYVIALTRRTTSGESTRPPVSDAEMAVWAAYMRTVTVCDPTRPEHPCPTSGEA
                     PEQPAATAG"
     gene            complement(34105..>35441)
                     /locus_tag="AC16QF_02080"
     CDS             complement(34105..>35441)
                     /locus_tag="AC16QF_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015502313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6447370.1"
                     /translation="SICIDTHGSAAQKSLYLPKLYSGRWSGAMDLTEPHAGSDLGMMR
                     TRAESAGDGTYRITGTKIFITSGEHDLSENIVHLVLAKVPGAPAGSRGISLFIVPKFL
                     PDADGEPGERNALASGSIEHKMGIRGSATSVMNFDGAVGFLVGEENQGLAAMFTMMNY
                     ERLSVGLQGLGAGELAYQQAARYATERIQGRSPEGPQKPEAAADPLIVHPDVRRMLLT
                     IRCLTEAGRAFGLFAGMQLDLGKHLGDPDALALGDLLTPIAKAFLSDRGLDCAVLGQQ
                     VFGGHGYVREWGMEQIVRDARIAQIYEGTNGIQAQDLIGRKILRDGGAVLRRLATIVE
                     ADARDSAYRADLLAAVARLVKVTDSLVQRSRADAALPGAVAVDYLDLAGYTLYAWLWA
                     RMADAAPGDDFGRSKRACAEFYFKRLLPRTLGLAAAIEAESEAVMALAADAF"
BASE COUNT         6164 a        12193 c        11376 g         5708 t
ORIGIN      
        1 gaggcgggcc tcggcatgca ggggatagtc ctcgtcatca acggagcggg gatagggaaa
       61 ggccggctgc agatatgcgc cgtcgcagta agaaggcaat cgggccgcga cgcgctccgg
      121 cagctcgtcg cggggcgtcc agtaccgttc cgccaccccc gtcaccggtg cggcgtcctc
      181 tgcagccgcc gccccggtga gcagtccggc caggagcagg cagcatccgg cacggcgtcg
      241 tgaggaaggg cggctcgtca tggccgcgga tgataaaaga tttacctccc cgggaataac
      301 ggcggccgcg ctacaattgc ccgggtggaa tcccgcagca taccaaccga actcgcgcag
      361 tggctcgccg gtgagctggc tcgcctggac ctcgacccga cgtttaccag ccaggcgctc
      421 ctggtcgagg cgagctaccg ctgcttctat cggatccgct cccggcatcg cggcggggag
      481 gcgctgagcc tggtggccat ggaatcgcct cccgagcgcg agaacaacca gcagttcgtc
      541 accctggcca aggtcttcgc cgcccatggc gtgggggttc cgcgcattct cgccgccgat
      601 ctcacggacg gtttcctgct gatgaccgac ttaggcgatc ggcacttcgc agacgcctac
      661 cagagcggcg agcgcgatcg ggccctggcc gccggcatcg acacgctcat ccgccttcag
      721 accgttgacg accccgccgt gccgctctac acggctcggc ggttccggga cgagctcggc
      781 atcttccggg agtggttcgt ggagcgctgg ctcggcgagc cgtttcctgc cgaggacctg
      841 gccggcattc cggagaggct cgtggcgtgc gtcgagcagc agctgcaggt ctgcgtccac
      901 cgggacttcc acagccgcaa cctgctcctg gcagccgatg gcagcgtggg cgtcgtcgac
      961 ttccaggacg ctctgatcgg gcccgtgagc tacgacctgg cctcgctgct gcgggactgc
     1021 tactaccgtt ttccggagga cgagatcgcg cggtggcggg attactatct ggaacggagc
     1081 ccccacgcgc tggaccccga gcagttcgct cgcgacctgg acctcaccgc cgcccagcgg
     1141 cagctgaaag cggtggggat cttcgcccgt ctcgacctca gggacggcaa gacgtcgcat
     1201 cttcgacaca tcccgcccgt gctcgaccat ctcgctgcgc tgtcggcccg gcacgacacg
     1261 ctcacgcccc tcggtcccca tctggccgtc tggcagaagg ccgctgaacg ccgcctgcag
     1321 gcccgccgat gagagcgatg atcctggccg ccggccgggg cgagcggctc aagccgctca
     1381 cggacgccgt gccgaaaccc atgctgacgg tggccggcga gccgctcatc gcccggcagc
     1441 tgcgcgccct gggagaggcc ggaattaccc gggtggtgat caacctgcac cacctaggcg
     1501 agcagatcgc gtcgttctgc ggttccggga aagactacgg ggtggccatc gactacagcc
     1561 gcgaggacga gctgctggac accggcggcg gcatcgtcaa ggcgctgccc ctgctgggcg
     1621 atgcgccctt cctgatactc aacggcgaca tcctcaccga cttccccttc cagacgctcg
     1681 ccaccgccct gcccgactgg gccgacctgc atctggtgct cacgtcccga ccggcccatc
     1741 gtgcccacgg cgatttcgat tgggaggcgg gcagggtcac cgggcgcggc gagtcctacg
     1801 tctactgcgg catcgccgtc ttgaggcccg agctcttcga cggccgcatc atcgagcctt
     1861 tctcgctgca gcagattctc ttcgacgccg tggccggcgg gcgggtctcg gccgagatct
     1921 ggaccggaac gtggatcgac atcggcagcc acgaccagct gcgcgcggcc aacgagcatc
     1981 tcaaatcaga accggggtaa aatgcgcccc catgagctac tggatcgcac cctccattct
     2041 ttccgccgac ttctcgcggc tcggcgcgga gatcgaagcc gtgctcggcg ccggcgccga
     2101 catcattcac ttcgacgtca tggacaacca ctacgtgccg aacctcacca tcggccccct
     2161 ggtgctgcaa tcgctgcgca aggccggcat cgactgcacc atggacgtgc acctgatggt
     2221 gaaacccgtg gaccggcttg tcggcgactt catcgaggcc ggggccgaca tcatcagcgt
     2281 gcatccggaa gccaccgagc atctgcaccg cacgctgtcg ctgattcgag acgggggggc
     2341 cagggccggc ctcgtcttca acccggccac gccgctgggc gttctcgagg aggtcctcga
     2401 tctggtcgac ctggtgctcg tcatgtccgt gaacccgggg ttcggcgggc aggcgttcat
     2461 cgcttccagc ctggacaagc tcgccgcagc ccgtaggctg atcgacggca gcgatcgtgc
     2521 catccgcctg gaaatagacg gcggcatcaa ggcggacaac atcgcgctgg ctgcccaggc
     2581 gggagcagac accttcgtgg ccggctcggc catcttcggc agcccggact acgccgccac
     2641 catcgcttcc atgcgcgcag cgctcgaggg cgtccaaccc gggaccccgc ggggagcagc
     2701 ttgaagccct acaccggcta tctattcgat ctggacggca ccctgatcga cacggcgccg
     2761 gacatcaacg ccgcgctgaa ccatgctctg acgcaatgtg gtgcgcccac ggtgaacgag
     2821 gccctgacgc gccactgggt cggccacggc gcccgggtgc tggtggagca ggcgctggcc
     2881 tatcacgagc tgcccgagga agacctcgcg cccatccacg aggcttttct tgcctactac
     2941 ggcgaccacg cagcggagca cagccgggtc tatccgggcg tggtcgacac cctgacggcg
     3001 ctcaaggagc gcggcgcgag gctcgccgtg ctgaccaaca agctgacccg gttcagcctg
     3061 cccatcctct cgaccctggg catcgacggc tacttcgacg ccacgatctg cggagacacc
     3121 gccgcggcgc ccaagcccgc cgcggacccg gccctgctcg cctgccgcga gctgcgcacc
     3181 acccccgggg acagcctggt ggtcggcgac tcgcagaccg acgtgctctg cgccagggcg
     3241 gccggatgcc ctgtggtctg cgtgcgcgac ggctacaatc acggcgtccc ggctgagcag
     3301 ctgggcgccg acgcggtgat cgagtcgttc ctggacctcc tttgatgggc agaccgtgaa
     3361 ccaggcccaa ttcgccgagc tggccgaggc cggctacaac cggattccag tggtcttcga
     3421 gaccctggcc gatctggaca cgccgctgtc gacctatctg aagctggcgc gcggcccgta
     3481 ttcctacctg ctggaatccg cccacggcgg cgagaagtgg ggccgctact ccatcatcgg
     3541 cctgccgtgc cagaccgtgc tcaaggtgca gggaaaccag atcaccgtgg agacggacgg
     3601 cgagctggta gaaacccacg aaagccccga cccgctgtcc ttcatcgagg aattccaggc
     3661 ccgctaccgg gtcgccgctc tgccggagct gccgcgtttc tacggcggcc tggtgggcta
     3721 tttcggctac gacaccgtgc gctacgcaga gcggaggctg gccgacggcg cgccccccga
     3781 ccctctcggc accccggaca tcgtgctcat ggtctccgag gacctggtgg tgttcgacaa
     3841 tcttcgtggc cggatgcagc tgatcagcct cgtcgacccg gccgagcccg acgtctacca
     3901 gcgcaccatg gagcggctgc gcgaccgggc cctggcattg agcgagcccg ttccgcccat
     3961 tcccgaaagc gcgaccaacc agccggtgct ggagagcgac ttcgcatccg agttcggcga
     4021 ggagcctttc aaggaggcgg tggagaagat tcgcgagtac atccgggccg gggacgtcat
     4081 gcaggtggtg ctcgcccagc gcatgtcgct gcctttcggc gccgacgcca tcgacctgta
     4141 ccgggcgctg cgcagcctca acccgtcgcc ctacatgtac ttcatggacc tcgaggaatt
     4201 ccagatcgtc agcagctcac cggagatcct ggcccggctc gaggacggca agatcatcaa
     4261 ccgccccctg gccggcaccc ggcggcgcgg gcacacgccc gaggaggacc tggccctgga
     4321 gcaggagctg ctgtccgacc ccaaggagct cgccgagcac ctgatgctca tcgacctcgg
     4381 ccgcaacgac gtcggccggg tctcggagat cggctccgtg gaggtcaccg agcagttcgt
     4441 catcgagcgc tattcccacg tcatgcacat ttccagcaac gtggtgggac ggctggcgga
     4501 aggcaagacg gccatcgacg tgctgcgcgc gacgctgccg gtgggcacgc tgtccggcgc
     4561 cccgaagatc cgggccatgg agatcatcga cgagctggag cccgtgaagc ggggcatcta
     4621 cggcggcgcc gttggttacc tggcatggaa cggcaacatg gatacggcga tcgcgattcg
     4681 caccgcggtg atcaaggacg gcacgctgta catcgaagcg ggcggcggcg tggtggccga
     4741 ctcggtgcca cgcctggaat ggaaggaaag catgaacaag gggcgtgcca tcttccgggc
     4801 ggccgccatg gccgagaatc gcttccggga tctgggctag aaagacgtcg tcgtgaatcc
     4861 gttcgttacc gcgaagccga accatctcaa ggaagcaaac gccactcgac ccaccgttgg
     4921 cgagtgaagc gtcacgcgga ggcttcgcgt cgcccgatca ccagcacgag caggctcggc
     4981 agaagcgtca ggttcgcgat cagagccagg gccatggcgg ccgcggtcag caacccgaag
     5041 aagatggtcg gaacgaagtt cgagaacacg agaacggaga agccgatgat caccgtcacg
     5101 ctggtgaagt acatggcgcg gccaatggtg gcgtgactcc acgccaccgc catgcgaacg
     5161 tcttcccgcc tgcggcgctc ctcgtcgaag cggtgcagat agtggatggc gtcgtccacg
     5221 ccgatcccga tgctgatggc ggcgatggtg atggtcatca tgtccagggg tatgcccacg
     5281 taacccatga gcgcgatgac gctggccgcc gcaatcacgt tgggaatcag tccgagcaac
     5341 gcgtagagaa aggagcgcag aaggatgacg aacatggcga aggtggccag gagcacgtag
     5401 acgagcgtat ccagctgaga gctgagcagc tgcttgagca tgctgtcgaa tagaaccatc
     5461 atgcccgtca cctgaacggc atcggctggc accgcgccct gggtcacggc gaaatcgcgg
     5521 atgtcggcgg ccagggcctg acggtcgaac gaccgcccgc tctcgatgac gcgcgcgttg
     5581 atccgcaact cgcccgtgga cggagacgcg tagggccgga tgagctcgtc cctcaagtgc
     5641 tcgggcagca cgtccagaac cgcggcgacc tcgaggttgg aaagcgcatc gccctcggtg
     5701 aactgcctgg ccagatcctc cagcgacgtc agcgagagaa cctttcccac ctccgggcgg
     5761 gcctcgacgt accggtggac gagcatcagc ttgtccagct tgtcccgggt gaaccagtac
     5821 cgctcgggat aggtctcttc ctccgagaag aagtcgtcct cttcctcgac caggttttcg
     5881 aacggcggga actcgaggac cacgtcgaag ggcaccgtgc cgcccagctg ggcatcgacg
     5941 aaggccatcc cctggttgat gtccgtgttc tctcgaaagt actcgaggaa gcggttgtcc
     6001 atggaaacca tgctcacccc gagggcggca aacgccgccc acacggcgga gatgaccacc
     6061 accagcggtg cgcgccagcg ggccaggtgg ctgaagacac gggtaacccc cagctcccga
     6121 ctcagcaggg acggccccgc gacccggccg agcagaagca gcagcgccgg gaagagggtg
     6181 aagctcgtga agaaaccgat ggcgatgccg atgcacatca tccagccgaa atcctccacc
     6241 gggacgatct ggctggcggt cagcgatccg aaggcggcaa tggtggtcaa cgcgttgtac
     6301 aggcacggcg caaacttgct gaccatggtt tgccggacca gctcgtccgc cgccatgtcg
     6361 ggatgcaggt gggcaagctc gcgatagcgg accacgagat gaatggtcag cgagatggtg
     6421 atgatcgcca gcagggagac gaagttggaa gagatgacgg tagccggcca ttgggccagt
     6481 ccgaggacgc ccacggtgaa gccgatggtg acggcgctgg ttacgaccgg aagcaggacc
     6541 cagcgcagcc gtcggaaaaa cccgtagagc gtgaccatca cgatcaccac gacgctgccg
     6601 ccaaaaacca cgaggtcgct cttcacgaag gcgatcatgt ctgccgcgat catcggcacg
     6661 ccgcccaggt ggagaacgcc tcgatccgcg tagcggtcgc gcacatcgcg tatctggccg
     6721 atcagacgtt cccggccgtt gacgtaggct tccctcaacg tctcccgggc aacctcgagc
     6781 tccgccagcc gcttgacctc cgccgcggac ctctcggcct ggcttcgcag ccgggcccgt
     6841 tcctcgtcga gcacgagaag ctcccgatcc agcgagatgt ccacccgcat ggccgtggtg
     6901 cgagcatctg ccgtgatgag gagcccgccg aacaccgggc tctccttgag ctcctccctg
     6961 gccgagggcc ggtccacgtc cgccgactcc agcgtgcgaa aaccccgggc cagctcggcc
     7021 agcggcacgg gaggactctt cagcaacggc gcatcgagaa tggagaagac cgagccgacg
     7081 ccctccagag cggctatctc ctgctgcaag gcgcgcaggg tggcgagatt ggccggatcc
     7141 agcgcgtcac cgtccctggg cgtgaacgtg agcagaagaa actcgtcgcc gccgaaagtc
     7201 tcggcgattc tcaggtaggt ggcgaggtcc ggatcgccct ccaccaccag cgtgtccgag
     7261 gaggcatcga agctgaatcg cgtcccgcaa acgcccgcga ctaacagcag cagcgccacc
     7321 agggtgagca gcgtgcgcgg atagttcagc gcccgcaggt aggtcgtcac cagcggttgc
     7381 aggtgtttgt gcagatcgga gatacgcgtg ctcgcggcaa ggaattttcg gctattgttc
     7441 ggtggtggaa aacattattc aactcataga ggaagtggcc gaggcccttc tcgagaaccg
     7501 ttcgcgcctg atcaccgccg agtcctgcac cgggggcatg gtggcagcgg agctcaccag
     7561 ccgacccggc agctcggagt ggttcgaggg ggcgctggtg acgtatcgtc tgagcgccaa
     7621 gcagcgcctg ctcggcgtgc cggcggcaat gctcgaccgc tacggcccgg tcagcgagcc
     7681 cgtcgcccgg gcgatggcag agggcgcgct gcgcgtcagc gatgcggacg tgagcgtcgc
     7741 gataacgggc attgccggtc ccgacggtgg cgacatgctg gccccagtgg gcacggtttg
     7801 gtttgcctgg gccaggcgcg acgagcccgg cgccgagccc gtctgcctgc aggcgactca
     7861 gcatcgcttc tcgggatctc gagacgaggt tcgaactcag gccgtcgccc tggccctgga
     7921 gggcgtgctc gcgagcctga gcgggatcaa cccttctcga tcttgatgct tcggccgccc
     7981 gggcttgcca ccttgggcac ccggatcctg agcacaccgc gccggtagct cgcctcggcg
     8041 ccatcggcgg tgactgccac tggcatgggg atcgtccgct gaaatcggcc gaacgcgcgc
     8101 tcccgaacca ccatggctcc ttcacgccgc tcggattcga ctctcttctg acccgtgatc
     8161 gtcacctgcc gctcgtccac ggacacgtcg atctcgtcct tctcaagccc cggcgcttcg
     8221 agctcgacgg tcacgtgctc gccgctttcc gacacatcgg cggccaggag cccccagcgg
     8281 atctcatcgg ggtcggccgg ctcgttgcgc cccggcgtaa agtatgtgag cgcgttgacg
     8341 gccttgtcgc tcaggctgcg ccaaccgtct gccagccact tccaggtctg ccccacatcg
     8401 gccttgatct gattgaggtc catggcgggc tcctagctga cgggcacgcg ccgggtctcc
     8461 ggtgcgccgc ctttgggaac gtgaatggtg agcacaccgt ccttgacgtt cgcgcgcaga
     8521 tcctccggcc tcgcgctcgg cggaacgcgg aactggcggg cgaacttacc atgacggcgc
     8581 tctacgcgaa cgtagtgctc gccctgcttg gtctcctcgg ccgaccgggc gccccgcacc
     8641 gtgaggacgc cctcgtgcag gtcgacgtcc aggtcctcgg ccccaaggcc gggaacatcc
     8701 atgaccagaa gatatccctc ctgctcctcc cggatatcca cggccggcag ccaatcccgg
     8761 ctccccgcgt gaggttcacg gtccgtcccg cccatcatcc gactgaacag gtcgaaggga
     8821 tctcccgatc gtacgggctc gctcatggct gctggctcca agttggtcta gagccgtaat
     8881 ggtgccccca gcgggccgaa tttcactaag tacttcccgc agcagccggt cactttttct
     8941 ttttcttggt atccagagcg gcttcgatca tggcgacctg ctcagcggtc acgggagaga
     9001 agtcacccag agagcaggta gcgctcgttg gctgccatcc gaaggaattg ctcaccgccg
     9061 tgaacgtgtc gaggttgcag atctggctgc cgcggatttc gaactgcagc acgtcgcgct
     9121 tgcgcaggcc gacgcacgtg ctgcgcagcg tgttgaccca caggcggttg ccgcgaccct
     9181 tgaagagcac gtgccgggta tccaggacat ctaccgagtc gtagaggttc gtggagatgc
     9241 agcgttctcc accgccatag tcttcctcgc cgagcggctg ggtcaggatc gtgtcgatct
     9301 gctcggaaac ctcctgctgc gccgccgccg tctccgccgg atcgctggcg cacccggtaa
     9361 gaagcgcgcc agcggtcagg gccgcggcca atcctttcgc catgccgttc atcgtcagtt
     9421 ccatgcttgc cttcctcaat cgcaaccggt gtgaccgtct gggcgtcgag tttatactag
     9481 ccggcttcca gtcaacgagc agccgcagcg cagcgggcac ggcaagcctg agggcagcga
     9541 acccgaacgt cccggcccac cggatactct tatgctcctg atgatcgaca actacgactc
     9601 gttcacctac aacgtggtgc agtatctcag cgagctggga gccgaggtgg ccgtgtaccg
     9661 caacgacgag atcaccctga ccgagatcga ggagctcgcc ccggagaaga tcgtcatctc
     9721 accggggccg tgcacgcccg acgaagccgg cgtttccatg gaggtcgtca cccggttcgg
     9781 cccgacgatt cccatcctgg gcatctgcct ggggcatcag agcatcggcc aggccatggg
     9841 cggcacggtg cggcgcgcca gggaggtcat gcacggcaag acgtcgctca tccaccacga
     9901 tgaccggggc gtgttccacg gccttccccg gccgttcgag gcgacccgtt accactctct
     9961 cgtcgtcgcg cacgaggacc tgcccgattg cctggaggtc acggcctgga cggagcgcga
    10021 cggcgagctc gacgagatca tgggattgaa acatcgcgag taccccatcg agggggtgca
    10081 gtttcatcct gaatccatcc gcacgccggt gggtcacgac ctgctgagaa actttctgga
    10141 ggggtgaagg acatggacat acaaggtgcg ctggccgctc tgttcgaaaa acgggatcta
    10201 tccctggagg acgccagggc ggtattcgtc gaggtcatgt ccggccacgc caccccggga
    10261 cagatcggcg cgctgctcgc cgcgctgcgc gtgaagggag agacgccgca agagatcgcc
    10321 ggggccgccg aggccatgcg gtccctgtcc accaaggtcg tcgtggacgt gccctacctc
    10381 gtggacacct gcggtaccgg ggggagcggc gccaagctgt tcaacgtctc caccgccgcc
    10441 acgttcgttg ccgccgccgc cggcgcccac gtcgccaagc atggcaatcg caagatgacg
    10501 agcttcagcg gcagcgccga cgttctcgag gccgccggcg tgaacatctc cctcgcgccg
    10561 gagcagatcg cgcactgcat ccgcgaggtt ggggtgggct tcctgttcgc gcaggctcat
    10621 cacagcgcca tgcgtcacgc cgcgcccgtt cgccaggagc ttggagcacg gacgatcatg
    10681 aacgtcctgg ggccgctgac caacccggcc ggcgcgcgca accaggtgct gggcgtcttc
    10741 agcgcggcct ggcagaccac cctggccgag gtcctcaagc tgctcggcgc gagccatgtg
    10801 ctggtggtgc acagcaacgg cctggacgag ctcgccctga acgggccgag cttcgtggtg
    10861 gagctcaacg ccggcaccat caccgagtac gagattgagc ccgccgactt cggcctgaag
    10921 ccttcctccc acgacgagct gcgagcggat tctccggaga ccagccttgc cctgctgaaa
    10981 cgctccctga ccgagcccga ctcatcggcc gccgacatcg tcagcctcaa tgcgggggcg
    11041 gcgatctacg cggccggcgt cgccacgagc ttcaagaacg gcgtggtcat ggcccaggac
    11101 gccatcgccg cagggctcgc cagcgaacgc cttgccgagc tcgttcgggt ctcttccatg
    11161 atggcggagc cgtgacggtg gaagagccgt gacggtagaa gagccgtgat ggtggcggcg
    11221 ccgtgaccat tctcgacgag attctcgccc acaaacgcac cgaggtggcc gagcgccagc
    11281 gggtgcgaag cctggattcc ctgcgcgccc tggcccaggc gtgcccgccc gcccgcggct
    11341 ttgcagatgc gctggaaagc aagcttttgg ccggcgcttc aggcgtgatc gcagagatca
    11401 agagagcatc gcccagcaaa ggcgtcatac gggaagattt cgaccccgtg tccattgccc
    11461 ggagctacgc caaggggggc gccgcctgcc tctccgttct taccgacgag cgctacttcc
    11521 agggccacga cgagtacctg caggccgccc gagcggccgt ggacctgccc gtgctgcgca
    11581 aggatttcgt ggtggacgca tatcagctgt tcgaagcacg cgcaatgggc gcggactgcg
    11641 tgcttctcat agcggccgcc ctcgacgcgg gacagctcaa ggccctgcac gaaacggcca
    11701 tcgcgctcgg tctggacgtg ctcgtggagg tccacgacgg ggaggagctc gagtcggccc
    11761 tggagatcga gcctcgcctg gtcggcatca acaaccgcaa cctgaagacc ttcgagacgc
    11821 ggcttgaaac cacctacgga ctcctgccgg gcattcccga cagcgtgacg gtggtgacgg
    11881 agagcggcat cgcaaaccgc gaggatgtcg cggccatgcg gcagcacggc gtgcactgct
    11941 tcctcgtcgg cgaagcgttc atgcgcgcgc cggatccggg gacggcgctc gccgagatgt
    12001 tcgcttagga ccgccgggct cgagccgcgg ccgcgtccct gcagcagaaa cggaaaacgg
    12061 gctctccgcc ggatctggca gagagcccgc tgttccacgt cctgtgtgag caaccgggcg
    12121 cgccgtttcc tttcggcgcg cctgctgggg tcttagcggg tgccgtaaac caccatcgtc
    12181 ttgcctttga cgttcaccag accctgctcg accagcgtct tcagaacacg ccccaccatc
    12241 tcgcgggagc atccgacgat tcggccgatc tcctggcggg tgatcttgat ctgcatgcca
    12301 tcgggatggg tcatggcatc gggctgcttg gtgagctcga tcagcgtacg cgcgacgcgg
    12361 ccggtcacgt ccaggaacgc cagatcgctg accttctggg tagtgcggcc gagccgatcg
    12421 gccatctgcc ggcccacggc gtagagcaga tcgctgtggc gctgggcgag ttccctgaac
    12481 ttgccgtagc tgagttcggc aacttcgcac tcggtcttgg ctttgatcca ggccgaacgc
    12541 ggcgcgcttt ccgtgaaaag cggcatctcg ccgaagaaat cgcccttgtt gagataggcg
    12601 acgatgattt cacgtccgga ttcgtcctcc acgagcacgg ttaccgagcc cttgaggatg
    12661 tagtaaatcg aatcgctctt gtcaccggcg tagatgagcg tgctcttggc cggatacttg
    12721 cggcgatggg ccgctgccag aaaggcatcc agatgcgtat cttcctgccc ggcgttcggg
    12781 ttgaacccct tggcggtcag tggttttccc atccctgaga actgtgaatt cttgttggca
    12841 agcatttccg ctccttgtga accttgcgga tgaatacttg catatcgttt acaaccacaa
    12901 cgagaactgg tcgacaaacg tatccatttc acagtatgag tggtccccca tcactactca
    12961 gacgctccca gcctcacgta tcgggcatcc agatatcgga aactgccggc catgcaagca
    13021 aaaatcacgc tgatcgagaa cgttcatttc gaagccgtgg cagactccgg gcacacggtg
    13081 ctcatcgacg gtccgccgga cgcgggcggc gagaacagag gtacccggcc gatggagctc
    13141 gtgctcatgg gtctgggcgg ctgcgcgtcg ttcgacgtgg tcagcattct caggaagtcg
    13201 cggcagcccg tcgtcggctg tgtgaccagg ctcacagccc ggcgcgccga cacggtaccc
    13261 caggtgttcg aggccattca tctgcacttc accatcagcg ggcagggcct ggacgaggcc
    13321 aaggtcgcaa gagccgtggc gctgtcggcg gagaagtact gctccgcttc gatcatgctc
    13381 gagcgcggcg gtgttgcaat cagccacgac tacgaaatac tggaagcatg aagctccgga
    13441 acctccgcag atgaccaaac cacccaagcc gcatgcaggg ctgcggcacg tggcgctgtt
    13501 caccaccacg ctggaagcct ccgtgcgctt ctacaccgag ctcctcggca tgcaggtgga
    13561 gtggaatccg gatcccgaca acttctacct cacgtccgga acggacaacc tggcgctgca
    13621 ccgggcaacc gagggccgct ccgtgcgcgg ccagaccctg gaccacatcg gcttcatcat
    13681 cgacgacgag gaagatgtgg acgcctggca cgaccacctg gccgccaacg gcgtgaacat
    13741 cctcgagaga cccaggaccc atcgcgacgg tgcgcgatcg ttctactgcg aggaccccga
    13801 cggcaacgtg gtgcagctca tctatcatcc gccgatttcc cgaccgggtg gtcagacgcg
    13861 gtaggtagtc tcggtcatca gcctggacgc cagcgacatc gcccgtttca ccggctgggg
    13921 aaactcggcc ccgccggctc ccagggcctc ggccccgtgg cgcgcttcgt cttcccgcat
    13981 ggcgcgaacg atctgacggc tgcgggaatc gccggccggc agggactgca ggtgcgactc
    14041 gaggtgtcgg cagacctgat cctccgtcgc ctccacgaat cccagactga ccttgtcacc
    14101 gagaacgccc gtcaccgccc ccagggcata ggaaagcgcg tagaaggccg ggttgagcac
    14161 gctcgggctg ctgcccagct cggcgagccg gccggcacac caggcgaggt ggtccgactc
    14221 ttcggaggct gccgcgagca gcgctgtccg ggtgcggggg tccgacgccg tcagcgcctg
    14281 gccctcgtag agcgcctggg cgcatatctc gccggtgtga ttcaccctca tcaggccggc
    14341 agcgtgctcc cgctcgccag ggctcatctc tccttcaggc acatcggccg cgggattctc
    14401 acggaccgaa gctgcagcgc ccgtcagcgt gcgcagcgct ttgtctacct gcgccaccag
    14461 ggcatcagcc aaagagccag gaccagccac gtcagcccag cgccatcagc tgccggccgc
    14521 ccagcaggtg gaggtgaagg tggaagaccg tctggccgcc gtgcccgttg cagttgagga
    14581 tcaggcggta gcccgactcc tgaatgttgc gctcggcggc gatggcccgg gcacagagca
    14641 ccagacgacc gacaagatcg gcgtgttcgg ccgcaaggtc gttcacggtg gcgacgtgaa
    14701 tcttcgggat gacgagcacg tgatgcggcg cctgcgggct gatgtcgttg aaggcgagca
    14761 gctcatcgtc ctcgtagacg atgtcgccgg gtatctcgcc ggacgcgatt ctgcagaaca
    14821 ggcaatcgga actcattcgc ttctcgcgct tcggtcaaca tgccgggaga ggtgactgta
    14881 aaccggcaca acagcccccg caacgcgggc ttaaagatga gcgcgcggct caggatggag
    14941 cgcgacttcg gcgtccagcc cgctctcggc aagcgccacg cgaaagccgc gatgaaggag
    15001 aatgaaggca gccagcacgc gctgccggag actgccaccc gccagacggt agcgcagctc
    15061 gggcgtcagc aacccgagcc gctccctgag cagcaggagg ggtataccca ggcccgccga
    15121 cacggaccgt ccgcctatgt cgatggtcgc ctgggcgggg ccatcgccgt cgtcgagaag
    15181 cacccgcccg accacgaagt cgcgaaacag ctcaccggag agcgtcatca cgcgaccgtc
    15241 ggagaccatg gtcacgcaag cgttgcggca cgcaccgctg atggccgcat cctccccgaa
    15301 atggtcaccc ggctcgagca gcgcaagcga acgccgccct ctgctgacct cggctcggcc
    15361 ggccgcaagc acgaagacgg tatcgccggg ctccccggtt cgcaccacca tctcgccccg
    15421 gaggaggtcc acggggcgca tggcccgcat caccctctga cagtggtcga ggctgacccg
    15481 acccatggct ccggagctga gaaagcggca ctcccacccg tcggtcaacc actggggccg
    15541 ggagagcggc ggctggactt cccgggcgtg ggccgtggcg gccgccgcat cttcgctcac
    15601 gcgcacgagg gtgaccggag atagggtgag cacgccctga gcgcccgggt agatgggcca
    15661 ccgggatcgc ggcgacccgg cagcaacccg ttggtcaggt tcccgcaaac gaaccgctcc
    15721 tcgctgcaga tagtagtgcc cgggcagcga ccggcctggc ctgaccagcc aacggcccgc
    15781 gggcaagatc aaggtgctgg cgtgctcggc caccggaatg agcgccgcgc tcgggagtcg
    15841 agcaaatggt tcgaagcggc gcagcgaccg gacaatcatg gcgccatttt cccgcggcgc
    15901 ggagctgcgg cgccgccgcc ttcagattgt tggcgtgacg gaaattgccg ttatctgtcg
    15961 agcgctcgat gcccgatgtc gcgccggtag tagcggttcc gccagtcgat gtgctcgacg
    16021 gctccgtacg cggccgcacg ggcgccggca acgtcgtccc caagccccac gacgcagaga
    16081 acgcggccac cgttggtcac cagcacgccg tcttcgagac gggtgccggc atgaaaggtc
    16141 ttcacgcgcg gatctgcgtc tgcggtatcg atgccggtaa tggcgtcacc cttgtcgtac
    16201 gcctcggggt acccccccgc agccatcacg acgccgagcg ccacccgcgg gtcccagtcg
    16261 agctcgacgc cggcgagttc cccatccagc gcctgccggc agagaccgac cagatcggag
    16321 cgcaggcgca tcatcacggg ctgggcttcc ggatcgccga accggcagtt gaactcgatg
    16381 accctgggcg caccgtcggg catgatcatg agcccggcgt agaggaagcc gacgtaggga
    16441 acgccgtcct ccgccatgcc acgcaccgtg gggttgacca cctccttcat gatgcggtca
    16501 tagacgccgg cagttaccac gggggcaggc gagtaggcgc ccatgccgcc cgtgttgggc
    16561 cccttatccc catcgtcccg ggctttgtga tcctgagacg tggcgaacgg caccacgttg
    16621 gtgccgtccg atatgcagat gaagctcgcc tcctcgccag ccaggaactc ctcgatcacc
    16681 acccgggatc cggcctcgcc aaaagccgat ccgctgagca tgtcgtccag cgcctgaagg
    16741 gcctgctcga tggtttccgc gaccaccacc ccctttccgg ccgccaggcc atccgccttg
    16801 atgacgatgg gcacgccctg ctcgcgaacg taggcctgag cggccgccag atcggtgaag
    16861 gcttggtagc gcgcggtcgg gatccggtga cggtccagaa acgccttggt gaaagccttg
    16921 gagccttcca gctgggccgc catcttgctc ggcccgaagc aggcgagccc gcggccctcg
    16981 aagtaatcgg taacgccttc cacgaggggg acttccggcc cgacgatggt gagccctacg
    17041 ccgttcgcgg cggcaaactc tgccagggcg gggaaatcgt ccaccgcgat gtcgacgttc
    17101 tcgcacccgg cctccgtcgc cgttcccgcg tttcccggtg ccacgaagac cgtctccacg
    17161 ccgtcgctct gagcggcctt ccaggccagg gcgtgctccc tgccgccgct gccaatgaca
    17221 agcacattca tctaggcatc ctctaaggtt cgatggcccc gcaaaggcta atgccggaag
    17281 tgacgtatgc cggtgaacac catggccatg ccgtgctcgt cggccgccgc gatgacctcg
    17341 tcgtcgcgaa cggacccgcc cggctgaacc accgccgcga tcccggcctc ggcagccgcg
    17401 tcgatgccgt cccggaaggg aaagaaggca tcggaagcca tcacggagcc ggctacctcc
    17461 agcccctcct cggctgcctt gaatcccgcg attcgggcgc tcaccaccct gctcatctga
    17521 cccgcgccaa cgccgatggt ctggccgtcg cgggcgtaga cgatggcgtt ggacttgacg
    17581 aaccatgcca ccttccaggc gaacagcagg tcgtcgagct cgctggcaga cggcgcgcgc
    17641 cgcgacacca cccgcagccc ctcttcaccc aggcaggtct cgtcgggcgt ctggacgagc
    17701 aacccgccac ccacgcgctt cagctcgagc ccgcgggagc ctgcctgcaa agggccggtg
    17761 acaagaagac gaacgttttt ccgggccgcg gcagcggcca ccgcctccgg cgccgcctcg
    17821 ggcgcgacga tgacctccac gaactgccgc tcgagaatca gcgtcaacgt gggctcgtcg
    17881 agcggtctgt tgaaggcgat gatgccaccg aacgccgagg tcggatccgt cgcgaaggca
    17941 cgttcgtatg cggcctgtac gtcggcggcc tccgcaacgc cgcacgggtt tgcatgcttg
    18001 acgatgacgc aggccggccg ctcgaacacc ttgacgcact ccagcgcggc atcggtatcg
    18061 gccacgttgt tgtaggacag gctcttgcct tgcagctggc gcagggcggc cacggtgccc
    18121 ggtcgcgccc ccgactcgcg gtaaaaggcc gcctgctgat gaggattctc cccgtaccgc
    18181 atggtctcca cgtggcgata gctgagcgtg agcgactccg ggaactcctg ctccgcggtg
    18241 aggtaaccgg ccacgagcgc atcgtagcgg gccgtatggc ggaaggcctt cgcggccagc
    18301 gcgcgccggc tctccaacgt ggtcgcgccg tcgcgctgca gggtctcgag cagatggccg
    18361 taatccccgg gatctaccac cacgagaacg tcgcgatggt tcttcgctgc cgagcggacc
    18421 atcgcgggcc cgccgatgtc gatgttctcg atggccagct cgtcggtgca gtcggcgcgc
    18481 tcgatggtgg cctcgaacgg gtagaggttg acgaccacga gatcgatggg ctcgatgtcg
    18541 tgctcggtca tggtcgcgcc atccacgtcg cgccggccga ggatgccgcc gtgtatcttc
    18601 gggtgcagcg tcttgacgcg gccatccatc atttccggga aaccggtgtg ggcggagacc
    18661 tccgtgaccc tgatgcccgc ttccgagagc gcccggtagg taccccccgt tgagaggatc
    18721 tccacgccca gagccgagag cccgcgggcg aactcctcga gccccgtctt gtccgacaca
    18781 ctcagcaggg cgcgggccac actccgcgcg tcagcgttgc tcatatgatt ccgtaaccct
    18841 cgagtttctt gcgcagcgtg ccccgattca taccgagcat ggtcgccgcg cgacttttat
    18901 tgccgccgat gcgcgccatc acgccgctct ccgaccgatg gcagaggccg gcagttcagc
    18961 caggaaatcc agctgggcct gcgcttgctc cagtcgattg aacgcctttc cgagacggga
    19021 gcggacctcc ctcggcccca gctggctcag gtgctggagg taccactgaa cgtgctttcg
    19081 cgcaatgcgg gtgccgggct caccgtagaa ctcgtgcagg gcggcgacat gctccgccac
    19141 gacggcgagc ttctcaccca gcgaagccac cctgggcacc ccgtccgcta cctcgccgag
    19201 caaccatggt ctgcccaggg cagcacggcc gatcatgacg ccgtcgacgc cggtctcctc
    19261 gagtatccgg cgcgccgacc caggggagtc tatgtcgccg ttggcaaata ccgggatgga
    19321 caccctggac ttgatggcgg ccacggtgcc gtgctccgcc tcgccgttga agcggcaggc
    19381 gcgcgtgcgg ccgtgcacgg cgatggctgc gacgccctcg tcctccatcc gctgcgcgag
    19441 ctccacggcg ttacgggaat ccggcgacca acccgtgcgc atcttcaccg agatcggaac
    19501 cggcacggcg gcaaccgcag cgcgaacgat ggcaacaacc agatccggct ccttgagcag
    19561 agccgatcct gcagccttgt tgcagacctt cttgaccgga cagccgaagt tgatgtcgat
    19621 cacctgggca ccttcttcga cgtgccgcgc ggcactctcg gcaacgtccc gggggtcggc
    19681 gcccgcgatc tggaccgcga agggctccac gccgtccacc cggcagcggc gcaggcggga
    19741 tttgggcgtg tgccagagcg cttcacggga gccgaccatc tcgccgacca catagccggc
    19801 cccgaatcgc caggccagcg cgcgaaaggg cgcgtcagtg atccccgcca tgggcgccag
    19861 cagtacgtcg ttgcgcagct tgaatggccc gatggcggtc acggtcaccc cttgaagaaa
    19921 aggggcgaca tcataacgtc accggaacga aagagaatag ttaacggcca tgtcgccggg
    19981 gtgcgcgacg gtcagctcga tgtgaacggg cgtccgcggg ggcatgtcgg tggccccggc
    20041 catctcccct gccagataag ttcccggctc gaatcgctcc tccttgaccg gcgagccgtt
    20101 gagcgcggta aagctcagct cgagcacggg aaagggctgc gcgaaggcgg cctggttgac
    20161 gatgatggcg ttgacgagca gggcatcggt tcgctccggg tcgcgccgca ccaccagatt
    20221 cttggcgagc aggctctcga cgtcgcgcat caccggcagc tcgcagccca cgacgccgca
    20281 taccatggca tacagcggcc tcacctgggg gtcgcgcgcc caggtgtcga actgaagcca
    20341 gagaacctgg gtcgccacca gcagcaccgc ggccgccccg gccagcggcg cccaccagcg
    20401 tctgcgcggc ggctgcgcat acagggctag cccctgggcc tccgccgcgg agagcttctt
    20461 gagggatctg gggacctcca gggcagcgcc ttgcccggct gcgctagggg tatcggcgct
    20521 ttcgggctct gccgcccgcg ctgcatggtc ggcttcgggg ggcgggggcg ggcccgccgc
    20581 atcccgggcg cccgtgactt gcgaggattc gtccggctcc gccgaaggcg gctgcgcctc
    20641 gtggcctccg tagaggtggc gcacggtagc ggttcgacgc ggggccggct ccgggacgcg
    20701 ggcaggcacc atgtggcgcg cgccctggaa gacgttgagg caggacccgc aacgcactct
    20761 ccctgcagcc gcctcgagct gcacgtccgt cacccggaat cgagtctggc agtgcgggca
    20821 ctccgtgatc atcgattcca gcttctgctc aatcatgact ttagacttcg tccgtccctc
    20881 gaccctgcct gcggcttcga tcagtcaccg gtcacgcttg cgccctgaga gcaacaccca
    20941 gtcgccgtca ggctgggggt cggcgaagcg gaatgaagga taccctgcta cgacctcgct
    21001 tgcctgcctg gcaagcagcc cggagagcac gatcatgcca cccggtcgga taagatccgt
    21061 gaagcggctc gcaagctcca gcagaggatt ggcaagaata ttagccacca gcacgtcgta
    21121 gtcaccgcgc ccgggtacct cgtccggtcc gaccgccctc agccgccggg catccacggc
    21181 attcgcccga gcgttttcct cggttgccag caacgcctgg ggatcgtgat ccaccgcgag
    21241 cacctgcgcc gcgccgagca gcgccgcggc aatgcccagg atgccggaac cgcatccgta
    21301 atccaccagc cgctggccgg caaggtcgtg agacgcaagc catcccagac acagccgcgt
    21361 ggtcggatgt ccccccgtgc cgaacgccag ccccggatcc agacgcagca cggttccgtt
    21421 gcgcgcctca gaatcccgcg ggaccaccca caaacggccg ccgaaacatt cagccaccgc
    21481 gctcgagcgc caggcatcca cccaatcctg atcctcgacg aagccctcgt cgacgtcgcc
    21541 atgggccgcc aggaactcac gaagcgcctc cacgtcggca tccgcggaga acagggccga
    21601 caggcggcta cgacgccaca gaggggtttc accgggggca ggctcgagga gcagctcatc
    21661 ggacaggggc gccaggctca cggacaccgc gcccagctcc agcaggcgat cgctcagggc
    21721 atcggcggcg tcgatctcga catcgagctc gaactttagc cagctcaccg ggtcaggcga
    21781 tgttgaacag cacctggccc gatgccacgg cctgtccgtt gccgaccgcg accgacacgc
    21841 aggtaccgga ctggtcggca acgatctcgt tcatcatctt catgctctcg atgatgcaga
    21901 ccacgtctcc gacctcgacc tgctggccca cttttagata aggctcagcg ccgggttcgc
    21961 tggcggcata gaaggtcccg gccatcggcg ccgtgatggt ccccgcaacg gccccgggct
    22021 gagcggcccg cacctgggca accggcgccg gcacggtgcc cgcgaccggc cgggcgatgc
    22081 ggatggcctc ttccccgtcg cgcacctcga gctccgcgat gcccgactcc tccaccagct
    22141 ctatgagctt cttgatcttc ctgagatcca cgatcagcct ctcctatcgc gccatggcag
    22201 ccaggagggc gaactcgtat cccttcgccc cgagcccggt gatcacgccg cgcgcaacgt
    22261 ccgacaggta ggattgacgt cgaaactcct ctcgggcatg cacgttggaa aggtgcacct
    22321 cgatgaaggg aatgcccacc ccgagcaggg cgtcccgcaa ggccacgctg gtatgggtga
    22381 aggcgccggg attgatgacg ataccctcca cgccctgggt cttggccccg tgcacggcgt
    22441 caacgagctc gtgctcggcg ttgctctgca ggcacgtggc ctcgttgccc gcctcccggg
    22501 ccagggcctc cagcctcgaa ttgatgtcct cgagggtgtc ggtgccgtag atctcgggtt
    22561 cccgggtgcc aagcaggttc aggttggggc catgaagcac gaggaatcgg gccatcgcag
    22621 gactccagac gtcacagggc gccgagtttg cagaaaccca cgcggaaaga caatacggat
    22681 aaaatgtgaa tttggacgtt aattaacgga agttaaccgc tatttcagag aagttatttg
    22741 atccaacccg ttccaggacc gcccccagat gggccgccag cgcgtccgcg ccgatttcgc
    22801 cctgcacggt aacttcggct aatttgcgac catcttcagc gaaaaagacc atgctgggag
    22861 gcccgaagac gccaagctcg tccaggagcg ctttgtggtg atcgtcattc gccgtcacgt
    22921 ccgcccgcag caggttgaac cgcgacaacc tggctgccac ctccggcttc gggaaaacgc
    22981 tgcgctccag cactttgcag gagatgcacc agtcggcgta gagatcgagc atcaccggct
    23041 tgccctcgcg gctggcagcc agcacggcgg cgcgcagccc cgccacgtca tccacgggct
    23101 gccactcgag cgcctcgcca ggctgatggg ccggatccca tgcggtgagg cccgccaggg
    23161 gcctgagcgg gtcgttgccg ccgccagccg ccccgacgag cagcaaagcg ccgtagacga
    23221 cgcccagcag ccccgcggcc ctgacggcgc ccgacaccat gccccggggg ctccaggcac
    23281 gcaggcccag gtagccgccg gcgccaatgg ccagcgccgc ccacagcgcg aggttcaccg
    23341 ggcccggcac cactcgctcc agcagccata cggccacggc cagcagcagc acgccgaaca
    23401 ggattttcac agcgttcatc cactcgccgg cacgaggcag catgtgaccc ccgctcgcgc
    23461 cgatcagaag cagcggaacc cccatgccga gccccagggc caggagggcg ccgcctccga
    23521 gcaccgcatc tccggtcgcg ctcaggtaaa tgagggcacc ggccagcggg gccgatacgc
    23581 agggcgagac caccaggctg gacagagagc ccatgacggc cacgctgagg tgtttgccgc
    23641 cgcccgtccg cgatcccacg gcgtcgatgg ccgactgcca gcttgccggg agccgcagct
    23701 cgtagaggcc gaacatcgac agagacaacg cggcgaaaac cagggcgaac cccaccagca
    23761 cggggggcga ctgcagcgcg gcctgcatgt tcaggctggc accgaacatt cccatcagag
    23821 tgccgatgat cgcgtaggtg aaggccatcc cgagcacgta tgccagcgag agcgtgatgc
    23881 cacggcttcg cgtgagcttc tcggaatcgc cgacgatgat gctggaaaga atcggcacca
    23941 tgggaaggac gcagggagtg aaagtgagcc ccacccccgc cacgaaaaag agcagaagcg
    24001 cggtcagcag gctgccctcg gcgagcaggc cggcaagggc ccgttcctcc gtctgaaccg
    24061 tcgcagcggg gctgccaggc gcgctgccgg catctccgcc ggatatcgcg cccggggcgc
    24121 ccggcattgt cagggtcacc caccgggtct ccgggggata gcaaagaccc agatccgcgc
    24181 atccctgata gccgatgccc acctccacgg ttgcgccgcc ctgaacgggc agccgggcag
    24241 tggctctgcc gtagtacacc tccacctcgc cgaagtactc gtcgaccttg gccttgcctg
    24301 gcgggatctc cggggcgccg agaacggcaa tcgccccacc acccgccggc tcgcgggcct
    24361 cgaaggtgaa tcggtgccgg tagaggtagt agccgtccgc gatgtcccag cgaacgagta
    24421 tcgcgccgtc gggcccgacc tcggcgctca tggcaaacgc ctcgtccacc gggagaaacg
    24481 tcggctgctc ggatagccac ccggcggccc ccgtggagat gtcgtccagg gccgacgcgg
    24541 ccgcaccggg cgcggccagc agcaaagtaa aaccaatcgc cgcggcgcca agccccggcc
    24601 agaatcgcct cattgttcga cggactctca tcacctcaat tctatccgac agctcgaccg
    24661 cattggagtt cgcccgcaga cccggcaata tagccgttcc cttggcccgc agaagtgaac
    24721 tggtcggttg cttcccagtg aacgaacact cccagcgctc tggctcgccc tgatgctctg
    24781 ggtggtggac gccgctggac tctcggcgca ggccggacgt gattccttgc ccgaaggagc
    24841 gcgggccgac cctctcaccg agcccgcgcc cgccccggcc cctcacgcgg ccgagataga
    24901 cgcgctgctg ctgcaggcca acgacgcaat ggaacgggga cagtacatca accccgcccg
    24961 cggaagcgcc ctgggcctct acgacagggt actggcgctc aatccgggca atgccgaggc
    25021 gcgcaggggc ctggagctca tcgctcagca ttatctcgac ctcgccagcg ccgcagtaag
    25081 gcggcgtcag ctgacccgcg cccggtccat ggtcgactgg gccagaatcg tggacgccga
    25141 acatcccggc atcgccgcgg ccgaggcgca gatccgcctg ctcgcagagg cgaggcgcgt
    25201 gcgcctgccg gtggcaggca gccagctgcg cgatcgcagc gacgcgctgg ctcggtcgtt
    25261 ggcggagctc ggatcacgcg cgcgcaacgg aaactgcctg gcgatcatcc ggtcgcccag
    25321 cgacgcctcg ggacgctgga tataccagca gatgagccgc gctccgggaa ctgcgcgcat
    25381 ccgggccgac atcgagatcg gctcaccgcc catggtggaa ctgctttgct tcgaaaacga
    25441 cgcctgatgc ccgatgccgc catattggcc gccgcgcttg ccgccgcgct ggccgccgcg
    25501 ccggttgcca tcccggccag cagcgcgccc gccctggcgg gttccgagct ggtcgtgacc
    25561 gatgcgcggg tccgggcgct gctgcccggc cagcgccata cggttgccta cttcgcgatc
    25621 gaaaaccgcg gccccgcaag cgttaccctg gtgagcgccg agagcgcggc cgccggggcc
    25681 atggagattc acgagtctcg ccgcgacggc gacatgtacc ggatgcggcg ccgagcagag
    25741 gttgagattc cagcccggac caccgtacgc ttcgacccgg gcgggctgca tctgatgctg
    25801 ttcgacgtcc aagcgctcgg cgagcagatg gaaatcgagc tgcgcacgag cgacgggcgc
    25861 cgcctgaagg caatctttaa tcgcatagca gcgggagcgg aatgaagatc aactggcgat
    25921 tctgggcttc ttcgaacgat gcggacgtca ccgacgggag gctcgcaact gccggtcgca
    25981 tcgggctggg cgccgttgcc gcctacctgc tcgtctgcgt gctgctgggc tggtggtggg
    26041 acaaggaacc cgacgtgttt gccgttcgcg agaacgcatc gagccgcgcg gagctcggca
    26101 acgagccgct ggtcaccggt tacatcacca ccgccaccct gatccgcctg ggcgaaaccc
    26161 tgctcgacaa acgcggtggc tatctttcca acgacgtctt tccgccgggc gtgtggatgg
    26221 acaacattcc gaactgggaa ttcggggtgc tcacccacat acgggacgcc acccgcacct
    26281 accgaaacga gctttcgcgc tcccagagcc agtcggcaga agaccccgac atcgtcagag
    26341 cggagggcaa gttcttcatc gacaacgcga gctggttcat ccctcaagcg gagtcggaat
    26401 atcgcgacgg catcaaagcg ctctcctctt acctgcagcg cctggccgac cccacccagc
    26461 ccgatgccca gttctacgcg cgggcagaca acctccagca gtggctgcag atcgtcgaag
    26521 cccggctcgg cagcctgtcc cagcgcttgt ccgccagcgt gggcaaacgg cagctcgacg
    26581 tctccctggc cggagatgct gcggccagcc aggccactga gtccccgcgg gagcaggagg
    26641 tcaagacgcc gtggctggaa atcgacgacg tcttctacga ggcccgaggc cagacctggg
    26701 cgctgctcca cctgctccgg gccatggagc aggactttgg cgacgtgctg gacaagaaga
    26761 acgcccgagt cagcctgcag cagatcatcc gggagctcga gcccacccag gacacggtct
    26821 ggagccccat catcctcaac ggcgacgggc tcgggttgct ggccaaccac tcgctggtca
    26881 tggcgagcta cgtctcccgg gcgaatgccg ccatcatcga cctgcgggcg cttctctccc
    26941 agggctgacc ggcggcgggt cccatacaaa ttcagacaaa tcccgccgcg cgctaggttc
    27001 ttattcgcag accgctccgt tagaattcgc gccgaaccga gcgctgcctc cgcgcgtatc
    27061 gtgcagcaac aagcacgggg tggagcagat cgcccgcgtt ccacacatgg tcatcgagtt
    27121 ggggaatcca atgaacaata ttgcgctgat accgccaata ttcggtatcg tcggcctgat
    27181 cgtagccttc ttcatcttca gcctcgtgaa gcggtacgac gagggcaccg aggaagtcaa
    27241 gcggatcggg gacgccattc acgagggggc catggtcttc atgcgccggg agtacacgat
    27301 gctggcgggg ttcgcggccg cgctgatcgt catcctctac atcgccctcg gaacagaaac
    27361 caccatcgcc ttcatcgtcg gcgcgctggc ctccgcgacg gcaggctgga tcggcatgta
    27421 cacggcgacc aaagccaacg tcagaacgac tacggccgcc cacaccgccg gcgcggccca
    27481 ggcgctgtcc gtggccttct tcgggggatc cataatgggc ctcaccgtcg cgtccatggg
    27541 cctgctcggg ctcggcatcc tctactacgt gttcggcggc gacccggaga ccgcgcacca
    27601 cattcacggg ttcggcatgg gcgcttccac ggtcgcgctc ttctcccggg tcgggggcgg
    27661 catctacacc aagagcgccg acgtcggcgc cgacctggtg ggtaaggtcg aagcgggaat
    27721 ccccgaggac gacccgcgaa accccggcgt gatcgccgac aacgtgggcg acaacgtggg
    27781 cgacgtcgcc ggcatgggct cggacatctt cgagagctat tgcggctcca tgatcgccac
    27841 catcgccatc gcctcgaccc tcgcgctggc agcactgacg gatctggcgc cgggcatgga
    27901 cgagggctcc gcccgggcgg ccctcatggg cctccccctc attctcgctt ccacgggcct
    27961 gatctgctcg ctggtcggca tcgcgctggt gaggctgatg tccgacaaag agcctgccgt
    28021 ggccctgcgt ttcggcaccc tgggcgcgcc ggtcatattc atcatcgccg cctggttcgt
    28081 gatcggccag gtgggtctgg aatccgcagt gtggtggtcg gtggtctccg gctccgttgg
    28141 cggcatcatc atcggcctgg tcaccgagta ctacacctcc tccaagccga tcatccggat
    28201 cgccgaggcc ggcgagacgg gcgcggccac ggtgatgatc acgggcctcg ccgtgggcat
    28261 gcagtccgtg gtggttccca tcctcgcgat ctgcggcatc atcttcgtgt cgacgcacct
    28321 cgccggcctt tacggcgtcg ggatcgccgc gctgggcatg ctcgggaccg tgggaatcac
    28381 catggccatc gacgcctacg gcccggtagc cgacaacgcc ggaggcattg ctgagatgag
    28441 cggtctgggc aaggaaaccc gggccatcac cgattccctg gacgagctcg gcaacaccac
    28501 cgccgccatc ggcaaaggtt tcgccatcgg cgcagcggcc ctggcggcac ttgccatcat
    28561 cgccgcttac gtagagacca tctcttacca ggacccgagc ttcaccctgc acatcggcga
    28621 ccccgaggtc ctgatcgggc tgttcatcgg ggggctgatc ccattcctca tcgcttcgat
    28681 cacgatgacg gccgtgggcg acgccgccat ggaaatgatc caggaaatcc gccgccagtt
    28741 ccgtgagata ccggggctgt tggagggcaa agcggatccg gacaacgcca agtgcatcga
    28801 catcgccacc caggcggcac tcaagcggat gctgctgccc ggcgtcatcg ccgtggcggt
    28861 gccgcccgtc gtcggcttcg gcctgggccc ggcggctctc ggcggaaccc tgggcggcgg
    28921 gctgctcggc tgcgtgctgc ttgccctgac catggctaac gctggtggcg cctgggacaa
    28981 cgccaagaag tacgtcgaga agggcaacct gggcggcaag ggctctgacg ttcacgccgc
    29041 cacggtggtc ggcgacaccg taggcgatcc cttcaaggac acatccgggc ccagcatgaa
    29101 catcctgatc aacgtgatgg ccatcgtcag cctggtcatc gcgccgctgc tggtctgatg
    29161 aacctgccgg ccccgctgca cgcggggccg gcccctcttc tctccggctt cagctcacct
    29221 ggaagggttc gagctttttc gccacgagct cgttcttcag ccgcacgtac tgtgggaggc
    29281 cgtttcgata ggcagggtaa tcctctccgg cgatgagcgg cgccaggtac cgcctgcctc
    29341 gcgccgtgat gccgaacccg tcctttgaga tgtagctctt cggcatcttc cgctcgacgt
    29401 tggccaccct ggaaagcttg acgctgccca ccgtccaccg atagggcgaa tcgctcttgc
    29461 gcaccacgat gggcatgacg gcgttttctc cggccagggc cagctccacc gccgcccggc
    29521 ccatggcgta agcctgctcc acatcggtgg ccgaggccac gtgcctggcc gcccgctgca
    29581 ggtagtcggc caccgcccaa tggtatttgt agccgagctc gtctttgatc atggccgcca
    29641 gcgtcggcgc cactccaccc agctgggtgt ggccgaaggc atccttgctt ccggcatcgg
    29701 ccaggaaggt gccgtcgggg tactgagctc cttcggagac gacgattacg cagtaaccgc
    29761 acccgtccac ggtctcccga accttggcga gaaaacgttt ccgatcgaag ggcacctccg
    29821 gcagcaggat gatgtgaggc gcatcgcgcg gcccttcctg ggccagggct cccgccgccg
    29881 cgatccaacc cgcgtgacgc cccatgacct ccagaatgaa gaccttggtg gaggtctcgc
    29941 acatcgacgc gacgtccatg gccgcttcgc ggatggatac cgcgacgtac ttggcaacgg
    30001 atccgaagcc gggacagcag tccgtgaacg gaagatcgtt gtcgaccgtc ttgggaatcc
    30061 cgatgcaggt gatcggatat ccgagccgcc cgcccagctg ggacaccttg tgggcagtgt
    30121 cctgggaatc cccgccgccg ttgtagaaaa agtaaccaat gtcgtgggct cgaaagacgt
    30181 cgatcaggcg ccggtactcg cgctcgttct cctctaagga cttcaacttg taccggcagg
    30241 atccgaaagc cccgctcggc gtcgtgcgca gcccggcaat gtccttggcg ctctcccggc
    30301 ccgtgtcgat cagctcctcg gcgagcgcgc cgatgatccc gttgcgtccg gcgaggacct
    30361 taccgatgcg atcgcgatgc ttgcgcgcgg tctggatgac cccgcatgcg gatgcgttga
    30421 tgacggccgt gaccccgccc gactgagcgt agaacgcatt ggctctcttc atccctcgac
    30481 gtctcgtccg gaaaacgagc ggtcattgtc cgtgaagaga aagttttgtg cgacaatttc
    30541 gccgatgaaa gccgtcttgc agcttttctg gcagatatgc cggctgaagc agagtcccga
    30601 gtacgtaccc acccagacgt ggttcgtgct gctggtcatc gccgcaaacc tgatctgcag
    30661 cgctgccgtt tctctgtccg ccgaaggcgc gcccggcctg ctgaccacca tcaacagcat
    30721 cgtcgtgggc cagacgacga cggcggccct ggtgtgggtg gcgttgaacc tgcgcgacat
    30781 gggtgggcgg ttccttgcaa ccatcacggc gctgttcggc tgcgacctca tcatcacggc
    30841 agtcttcgcc ctcatcctgc cgctcaccct gctcgcaccg accatggcgc ctgtgctgtt
    30901 cctgctcttc gtcgcctgga gcgtgagcgt tgccggcttc attctgcatc gcgcgttgca
    30961 ggtcccgctt gcgctcggga tcgtcgtggc cctgggcatg tcggttctca gcctggcatt
    31021 gagccagctg gccatcggcg catgaacgca gaacgccgaa tctattttct gggcatcggc
    31081 ggcaccctga tggggagcct ggcccagctg gccagggaga tgggataccg ggtctccggg
    31141 tcggacgtgg ccatctaccc gcccatgagc gatcagctgg cggccgccga catcgaggtg
    31201 ttcgaaggct tcgatcccgc ccacctggat cccgagccgg agctggtggt gatcggcaac
    31261 gcgggcctgc ccaggggtca tccgggcgtc gagcacatca tcgagaacgg gctgcgctac
    31321 gtgtccggtg ccgaatggct cggcagccaa gtgatgcccg gccgctgggt gctggccgtc
    31381 acgggcaccc acggcaagac cacgacggcc agcatgctgg cctggattct cgaccaggcg
    31441 gggctgaagc ccggctacct gatcggcggc gtgcccaaga actttccgcg ctcgtcccgg
    31501 ctgggcgacg accccttttt cgtggtggag gccgacgagt acgactgctc gtacttcgac
    31561 cgcagatcca agttcgttca ctaccatccg cgcaccctga tcatcaacaa tctggagtac
    31621 gaccacgcgg acatcttccc caacctcgcc gccatccaga cccagttcaa tcacctgctg
    31681 cgcaccgtgc ccggccacgg gctcgtcatc gcgccgtcca acgacgacaa cgtcaacgag
    31741 accctccagc agggttgctg gacgcccatc gcccgcgtgg gcgcacccgg ggtgaagcaa
    31801 ccgctggaac gcgacaacgg tgagcggtgg agcgccagca ggggagacgg cacaggtgct
    31861 gagttcgacg tctgcctggg cgaggaaaat ctgggcacgg tgcgctggcc catcttcggc
    31921 gacttcaacg tccgcaacgc cctggccgcc atagctgccg cccggcacgc cggcgtgccg
    31981 ccggaggtcg ccatagacgc cctgggcagc ttcgaaggcg tcaagaggcg cctggagctc
    32041 attgccgaga tcggcgcaac gttgatctac gacgactttg ctcaccaccc caccgccatc
    32101 cgcgcaacgc tgcagggctt gcgcaacaag gtaggcagcg aggagatcat cgccgtcatc
    32161 gagccccgct cccacaccat gtcactgggg accctgcgta acgagctcgc cacgtgctgc
    32221 tcgccggcgg acgaggtcta ctggtttcgc ggggagaaca ttcagtggga cctctcggag
    32281 ctgctgaaga gctgcgtgat ccctgcgcac atcaatttcg agctcgacaa gctcatcgag
    32341 gccctggccc agctgcccca gaagcgccgc cacatcgtga tcatgagcaa cggcagcttc
    32401 ggcgggatct accaaaagct cccaacccgt ctgcgcagcg gctaaactcg ggcaccactt
    32461 ccagcccact ttcgccagga gaccccgcca tgcaatccag accggcatgc ttcgtcgcag
    32521 cgctgctgct cgcattcgtc cctgctgcca acgcagcgga ttggtacccg tcgcgctacg
    32581 gcgccgacga caccctgggg gccatcaaca acctgtctcc tgagaaggtc gttcaggcgg
    32641 cccgtctggt cacggaaggc aagacctacc cgctgggggt ggagaccggc cccgactcgc
    32701 ccgcctaccc gccgcgaagc tattcgctca ccgtgctgca gcccggcgac ggcaccggat
    32761 ccctgatggg taccaacagg gcgtccggca acgacgatct gctgagtttc tggatgggca
    32821 tcggcagcca gatcgacggg ctcggccaca tgggcatcga gcacgtgtac tacaacggca
    32881 accgggccca ggatttcgtg agcgcaaccg gcctgtcgag gctgagcatc gaccgcctgc
    32941 cgcccatcgt cggccgcggc gtgctgatcg acatggccgc ccacatgggc aaggccattc
    33001 tccccgccgg caccgccttc aacagccgag agatcaaggc cgcagccaag gcccagggcg
    33061 taaccatcgg cacgggagac gtggtgcttt tccacaccgg ctggctgaac gtcgccgatg
    33121 acaaagcgcg cttcatggcc ggcgagcccg gtctgggcgt agacggggca cgctatctgg
    33181 ccggtctcgg cgtcgtggcg gtggggtcgg acacctgggg gctcgaggtg ctgccttccg
    33241 aggtcgaagg cgaagccttt ccggttcatc ccgagctgct cgccaagaac ggcgtctaca
    33301 tcctggagaa catggacacc cgggccctgg ccgcggacgg cgtgcacgag ttcctcttcg
    33361 tgctcggaca gcctcgcttc gtgggcgccg tccaggcggt gatcaatccg gtggcgatca
    33421 agtgatgaag gccccgaggc gcccgaccgc ccaggccgcg atccgggcgg ccgccgtggc
    33481 cggcctgctg cttgccggat gcgacaccgg ctccggcgcc gaggacaccg cgcaggcaca
    33541 ggcccgaacc ggcgccgagc cggcttcccc ggagctgctc atggcccgac caccggaggg
    33601 ctggaaggta tggtctgcca cccaggatcc ggggctgcga gtggcccaat acgtcccggc
    33661 cgacaccgac gccgacaact gggagcagat gcttcgcctg gaatccatga cgggggatcc
    33721 gctgccggat cccatcgaat ttctgacggc gctgggcagg gatcaggcgg ccgggtgcgg
    33781 acagtcatcg gatctgaaca ttcattccgg ccaggagaac aactacgcca cgtccgtccg
    33841 gctcttctcc tgtcgcaacg acgcggctcc ggacctggac caggtgaccc tgatcaaagc
    33901 catccgggga aacgagaatt tctacgtcat cgcgctgacc agacggacaa cctccgggga
    33961 gagcacgcga ccgcccgtca gcgatgcgga gatggcggtg tgggcggcct acatgcgcac
    34021 ggtgaccgtc tgcgacccca cccgtcccga gcacccctgc ccgacgtcgg gcgaggcgcc
    34081 cgagcagccc gccgccacgg ccggctagaa cgcgtcggcg gctagcgcca tgaccgcctc
    34141 ggattccgcc tcgatggcag cggcgaggcc cagcgtccgc ggcaaaagcc gcttgaagta
    34201 aaactcggca caggcccgct tgctgcggcc aaagtcgtca ccgggggccg cgtcggccat
    34261 tcgggcccac agccaggcgt acagggtata accggcgaga tcaaggtagt cgacggccac
    34321 cgctcccggc agagcggcat cggcgcgcga acgctgcacc agagagtccg tgaccttcac
    34381 cagccgcgcg acggcagcca gcagatccgc gcggtacgcc gagtcgcggg catcagcctc
    34441 gacgatggtg gcgagccggc gcagcaccgc accaccgtcg cgcagaatct tccgcccgat
    34501 caggtcctgg gcctgaatgc cgttggtgcc ctcgtagatc tgcgcgatgc gggcatcccg
    34561 gacgatctgc tccattcccc actcacgaac gtatccgtgc ccgccgaaga cctgttgtcc
    34621 caggacggcg caatccaggc cgcgatcgct cagaaacgct ttggcaatcg gggtgagcag
    34681 gtcgccaagg gccagggcgt ccggatcacc caggtgcttg cccagatcga gctgcatgcc
    34741 ggcgaagagc ccgaacgccc gacctgcttc agtgagacat cggatcgtga gcagcatgcg
    34801 gcgcacgtcc ggatgaacga tcagcgggtc cgcggcagcc tcgggttttt gcggtccctc
    34861 cggggagcga ccctgaattc gctcggtggc ataacgcgcc gcctgctggt aggcgagctc
    34921 ccctgcgccg aggccctgca ggcccaccga gagccgctcg taattcatca tggtgaacat
    34981 cgcggcgagg ccctggttct cctcgccgac gagaaagccg accgcgccgt cgaagttcat
    35041 gaccgacgtg gcgctgccac gaatgcccat cttgtgctcg atggatccgc tggccagcgc
    35101 gttgcgctcg ccgggctcgc catcggcgtc gggcaggaac ttcggcacga tgaacagcga
    35161 tatgccccgg ctgcccgccg gcgcccccgg aacctttgcc agcaccagat gaacgatgtt
    35221 ctcggacagg tcgtgctcgc cgctggtgat gaagatcttc gtgcccgtga tccggtaggt
    35281 gccgtcgccc gccgactccg cccgcgtgcg catcatgccg agatccgagc ctgcgtgggg
    35341 ctcggtgagg tccatggcgc cgctccagcg gccgctgtag agctttggca ggtagagcga
    35401 tttctgcgca gccgagccat gggtgtcgat gcagatcgag g
//
LOCUS       DBNHLM010000042        41263 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_5730, whole genome shotgun sequence.
ACCESSION   DBNHLM010000042 DBNHLM010000000
VERSION     DBNHLM010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 41263)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 41263)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41263
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_5730"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="AC16QF_02085"
     CDS             complement(<1..559)
                     /locus_tag="AC16QF_02085"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6447371.1"
                     /translation="MSVRRALVTGAAHGIGAAIATRLGSAGYRVGVLDMDGTAAAEVA
                     AALPDALPLTADVTDADSIDRALDAFGEPPNLLVNNAGIVHFGPVVEESVADFVKVIS
                     VNLIGCYVASRQCAQRMSGGDHIISITSINDRTPAPGSGAYPAAKAGVRQLMRQLAVE
                     LAERGIRANSVCPGFIDAGMSAPFYA"
     gene            complement(556..1758)
                     /locus_tag="AC16QF_02090"
     CDS             complement(556..1758)
                     /locus_tag="AC16QF_02090"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6447372.1"
                     /translation="MDLRITDYRHAHQALCSPHLKQSLYDAGGVIMEDTLLTLHGQRH
                     TRRRHLALRVFRRDYARHYEREVFPKALRDALEPLRGQPSIDVVAFGHAVSMNLTADF
                     AGIDRSEDPEATAALQRLTKKFGEGATLVHSTRAHDQVRAEVREALAEYRGRFLEPSM
                     ARRERLISAWRGSEIPEESLPRDMLTVLLTDRGGLDDDGLAREIAFYLQAGAHSTSNA
                     FAHALWEVLTWLGSDLARRAALAGDALATQRAVHEALRLHPASPVAWRRALEPIDFSW
                     GLHVEPEDHLVVDIAAANRDPEVFGPEADAFQPGRAVLDRRANPFGLTFGIGVHNCLG
                     RDIDAGLPQDSDTEAGSHQYGTVTLLVHHFLSELDAMFDPADLPVPDEDTSRPNWGRF
                     PVRVRWQT"
     gene            complement(1760..2956)
                     /locus_tag="AC16QF_02095"
     CDS             complement(1760..2956)
                     /locus_tag="AC16QF_02095"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6447373.1"
                     /translation="MSLVTVDSYQAANAALKQKDLRQALYDEGAVIMREVLVNLHGDE
                     HRTRRNVETQVFRRDFFRYYEHEVFPRTLEETIAPFLAAGKMDIVDFGYRVMVNLTAD
                     FTGIDRPKKSPEETEQLIKMLRLFGRTATLAHSTEDREPIRRAALDALADFQRDYVDP
                     SVARRQALIASVNRGDLSEEDLPRDILTVLLKNQDTVQLADDVRVREMAFFALAGAHT
                     SIHTLGHVMHEIFTWCASHPEDWSRFETDPLFVQRAVHESIRLHPSSPIAARRALCPV
                     HVAGKDVQADTRVNVDLQKANRDPAAFGADAERFNPDRPRGAKVPPYGLSFGLGMHAC
                     LGLNLAAGALPKPDTDPDDHHYGTVAMIVRTLLAHRARPEPGSPPRKDTTTARDIWAT
                     YPVTLG"
     gene            complement(2953..4257)
                     /locus_tag="AC16QF_02100"
     CDS             complement(2953..4257)
                     /locus_tag="AC16QF_02100"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase family protein"
                     /protein_id="HIR6447374.1"
                     /translation="MKTLLKNANIIDGLGNAPFAGDVLIDEELILAVGAADGEGATVI
                     DVAGKTVMPGLIDAHCHVTLDETHSNDEMFFHRREGLGALVAAYNVQKLLRAGVTGFL
                     DADCIFDIGVDLRDAIEAGIVPGPRMSTGGNALLTSVGGTAGNLIPAEGRVGYAKVVR
                     SRDEIVTEVRRQIKLGVDWIKVHVTGLVPRQKHRGEIQAWSFDELKTVCDTAHELGIP
                     VVGHCRNAKSIADSVRAGMDMILHATFMDDAALEAVIERKVPLVPTFTFQANLADFGD
                     LVGADPNLREIFRREIAESADMIRRAYEAGVPVLCGTETGFSLTPYGEWHYREMEVFV
                     RDMGFTPLEAIRAATSECGRAIGLQDRVGAVEAGRLADLLVVDGDPSKDVTVLGDKTK
                     IERVFLNGAQLDLPPLPARKPIPGWRVSAYSSSQLFWADAHK"
     gene            complement(4254..6677)
                     /locus_tag="AC16QF_02105"
     CDS             complement(4254..6677)
                     /locus_tag="AC16QF_02105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6447375.1"
                     /translation="MSTAKEPHGPAGGVSRPDEQAVPSALPRAFGASVKRKEDRRLIT
                     GRGNYTDDLLLPDQAFAAFVRSPHAHAAINSIDAEAAREMPGVVAVLTGEEFAASGLG
                     PLMCGWMIHSRDGEPMKVGEHPALASDKVRYVGDAVALVVADSLQAARDAAECVYVDY
                     DDLPAVADVVSAGSVDAPELHSGIAGNEAFDWEIGDADAVAAAFARAAHVTRLRLINN
                     RLVPNAMEPRSLNAQFDVGTGQHTLHITSQNPHGLRMTLAAVIGFAPEHKIRVISEDV
                     GGGFGSKAFNYAEEVACLWAARETGRPVKWAADRSEAFLTDAHGRDHVTTAELALDAD
                     GRFLALKVHTQANVGAYLSTFGSLIPTYVYAPLLSGQYEIPAIHANVIARYTNTAPVD
                     AYRGAGRPEAGYVVERLVEEAARELGIDSAELRRRNFVRSFPYVTPVEMEYDTGDFQA
                     TLDQALQLIDRDGFPARRAASEEQGKLRGLGIGAYIEAAGIGPSAKLGKLGSGAGLWE
                     SAEVRVNPTGSVEVLTGCHSHGQSHETTYAQLVADRFGIAVEDVDIVHGDTDKVQFGM
                     GTFGSRSGPVGLSAIAISCDKVVAKAKLIAAQVLDVPTQSVSFADGEFSSPQSNQRLS
                     FAEVALNAYTAHHFDTTQIEPGLKEGCYFDPPNFNFPAGCHVCEVEIDRETGVVEIVD
                     FVAVDDFGTLVNPMIVEGQVHGGVAQGIGQALLENTVYDDGGQLLSGSFMDYCMPRAD
                     DLPDIRVGFTNTPSPSNPLGIKGCGEAGAIGAPPAVINAITDALGVRELDMPATSEKI
                     WRVIKEQAL"
     gene            complement(6811..7908)
                     /locus_tag="AC16QF_02110"
     CDS             complement(6811..7908)
                     /locus_tag="AC16QF_02110"
                     /inference="COORDINATES: protein motif:HMM:NF053593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3169 family protein"
                     /protein_id="HIR6447376.1"
                     /translation="MLTKADDYPIHQTPEPIAYSGTDRNFYDRYFFNGYQREGELFFA
                     AALGVYPHLNVMDAAFSILVGGRQYNVQASKVLHMERLNTQVGPIEVEVIEPLNVLRV
                     SCADPANGVAADVCFRARVPAVEEPRFTRRQGSLTFMDYTRLTQNGSYEGWLEVDGQR
                     YELTHDETWGTRDRSWGIRSVGARDPQPNPYAGEPQFYWLWAPLNFPSTNNYYHLNAD
                     REGRPWNTMGRLADPERSEEMAAVASSIRHRSGTRLAESATLEYVRENGEEATIELQP
                     TQTFYMRGLGYMHPEWGHGLFHGELATNYEVYETAEIADADPGYFHVQAICDAQLTDA
                     RGSERGKGVLEQLVIGAYEPYGFRELFDVAP"
     gene            7945..8280
                     /locus_tag="AC16QF_02115"
     CDS             7945..8280
                     /locus_tag="AC16QF_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447377.1"
                     /translation="MDWERYKQLCDQPNVFSRWMLNQTIELIGESPVADTLAGVLGSF
                     PLEKPDDHRGGPATDMFVLELESGVRRAVVELVLLAREAGAATEATSHRGLGGFVEAW
                     REYLNWTRG"
     gene            8359..8745
                     /locus_tag="AC16QF_02120"
     CDS             8359..8745
                     /locus_tag="AC16QF_02120"
                     /inference="COORDINATES: protein motif:HMM:NF019473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HIR6447378.1"
                     /translation="MSDDKAVVLELIDGFNSIDLDRIVACFTEDAVYDNIPMQAVQGT
                     EAIRASLEGFMGAASEVQWDLLNVVGEGGVVLTERIDKFKVNGTWIELPVMGTFEVAD
                     GKIKAWRDYFDLAQFQTQFAKATGAQ"
     gene            8762..10129
                     /locus_tag="AC16QF_02125"
     CDS             8762..10129
                     /locus_tag="AC16QF_02125"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR6447379.1"
                     /translation="MFRRLLIANRGAIARRVVRACNDLGIESVAVYSEADAHAPYLSE
                     ASRAVGLPGVSAGETYLNTAALLEAVRASGADAVHPGYGFLAENADFARAVAAAGATF
                     VGPDPKWLEVMGDKVAARRLLADHGFPVFPGSGGLSSLDEALSAAAELSYPVLLKPAA
                     GGGGIGMRLVRDEAELARAFASAQRLAEQAFGQGGIYLERAIDKPRHVEFQILADGAG
                     GALHLYERECSIQRRHQKIIEEAPAVGLPREELDALAARAEAVMAEIGYDNVGTLETL
                     CTQQGEFGFLEMNARIQVEHAVTEAVTGLDLVGLQIRLAAGAALPERPGLCGHAVEAR
                     IYAEDPRTLLPDTGVLRVFRAPRMHRVRVETGYQEGQAVTPFYDAMLAKVIGWGETRE
                     LAIGRTLVGLKGFEIRGVKTNIPLLLDVLQDDGFLAGLIHTGFLDGLVLGSDLRSGSG
                     VGRNA"
     gene            10139..10363
                     /locus_tag="AC16QF_02130"
     CDS             10139..10363
                     /locus_tag="AC16QF_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HIR6447380.1"
                     /translation="MARYEVESEVQGNVWKIEVGEGDRVSAGDVLIILESMKMEIPVE
                     APVSGVVAELRVREEEAVEEEQILLVLEAD"
     gene            10420..11223
                     /locus_tag="AC16QF_02135"
     CDS             10420..11223
                     /locus_tag="AC16QF_02135"
                     /inference="COORDINATES: protein motif:HMM:NF005699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIR6447381.1"
                     /translation="MSELVTIDVSGGVADVRLNRPEKYNALSPQMFDAIVAAGERLAQ
                     AKDVRAVVLSGNGRGFCAGLDMASFQGMTETSPRSSAGSLLQQDDRPENRAQRPALVW
                     KRLPVPVIAAIHGVAYGGGCQIALGADIRFAAPDAKMSIMEIKWGLIPDMSITQTLRD
                     VLPLDLAKELTFTGRVLSGAEASQLGLVTHVVEDPLAAALELAREIAGKSPHAVRAGK
                     KLFEESWHADARTGLELEAALQTGLIGSPNQVEAIKANFENRKPDFRDD"
     gene            11236..12789
                     /locus_tag="AC16QF_02140"
     CDS             11236..12789
                     /locus_tag="AC16QF_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR6447382.1"
                     /translation="MSWETEVGEIERRRHLARQQGGPEGIARQHAKGRLTIRERIGEL
                     LDEGSFREQGKATAIPDYDENDELTGFVPANYVVGFGQVEGRRVVVGGEDFTLKGGSP
                     NAAGLRKSVYAEHLASEYRVPLIRLLEGGGGSVKGSGRKGGTVGEPVYADPRFRIIAD
                     TMTEVPVASGAMGAVAGFPAGRLVASHFSVMTRHTAQVLIGGPALVERALGVRLSKEE
                     LGGVQVHALSGVVDNIAEDEHDAFRQIRRFLSYLPGNVWQRAPRAPSDDDPTRMEQEL
                     LSAVPRDSNAPFDMRVIVEMIVDLGSFFEIGATFGPSQICGLARLDGQPVGVLANDNR
                     HYAGAMTAQAAQKYRRFVEMCDTFHLPIVNFVDQPGFMIGPEAERAGTIRYGMAAVAA
                     AVQATVPWASIQVHKGFGVATAAHYGKGAYVLAWPSVETGALPLEGGVAVAFHREIAA
                     SDDPDAKRAELESGMRAARSPFPRAESFAVHELIDPRETRPMLCEWVDWIQPRLDNLT
                     GPVRFGIRP"
     gene            12865..13737
                     /locus_tag="AC16QF_02145"
     CDS             12865..13737
                     /locus_tag="AC16QF_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6447383.1"
                     /translation="MTYSFEGKVAIVTGAGGGLGRCHALELARRGAKVVVNDLGGAMD
                     GTGGSSAAADEVVAEIKAGGGEALANGGSVSDRQGAKSMVVDAMNAWGRVDVLINNAG
                     ILRDRSFGKMELDDFQIVVDVHLMGAVNVTREVWPIMKEQNYGRIVMTTSPTGLYGNF
                     GQTNYGAAKLGQVGFMNSLKIEGAKNNIYTNTIAPVAATRMTEGLIPEGSQVALGPEL
                     VTPAVIFLCSEEAPNGVIVQAQGGNYSVACIVENEGVNLGLDATAEDVGANFEKISDL
                     TGAKPRGMLQLSSM"
     gene            13777..13965
                     /locus_tag="AC16QF_02150"
     CDS             13777..13965
                     /locus_tag="AC16QF_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447384.1"
                     /translation="MAGVILTLILAFVAGGALWIGIGPRLKLAEDAERNGLLNLLAYV
                     AIALPVAFAVVFYLLELI"
     gene            complement(13984..14115)
                     /locus_tag="AC16QF_02155"
     CDS             complement(13984..14115)
                     /locus_tag="AC16QF_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447385.1"
                     /translation="MHDENEREPVEELFAAEAPFAFPDQLYNEDDAEDNLSYVRGYN"
     gene            14238..14852
                     /locus_tag="AC16QF_02160"
     CDS             14238..14852
                     /locus_tag="AC16QF_02160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6447386.1"
                     /translation="MKLPLDPDEIKGFLHPEEGARLYALASVASGLGPCLEVGSYCGK
                     STLYLGTACRDQGQVLYAVDHHRGSEEHQPGEAYHDPELFDAEIGSMESLMAFRRTMY
                     RAGLEGCVIPVVAASALAGRHWATPLALVFIDGGHSVEAAQTDYRTWAPHVVPGGFLA
                     IHDLFPDPLEGGQAPIDVYRLASASGLFEPLETTRTLGVLRRVG"
     gene            complement(14784..15851)
                     /locus_tag="AC16QF_02165"
     CDS             complement(14784..15851)
                     /locus_tag="AC16QF_02165"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HIR6447387.1"
                     /translation="MTRPESLHRTARYIASVQQNDGAIPWFAGGITDPWDHVEAAMGL
                     SIGGHHAQAAAAYRWLARMQRADGSWLAAYSGARVADGTRAETNFVAYVATGTWHHYL
                     ISEDGKFLRSMWPTVKRALDFVTGLQAPTGEIYWAQDTRTGINKDALVTGCSSIYKSL
                     ECGIFMAAELNEDPGAWPACRARLGDAIILRPDRFDRTWESKSRYSMDWFYPVLAGLF
                     SQADARERLDERWETFVAPSLGCRCVSDQAWVTVAESCELAIACVASGEPDKAEELCD
                     DLARFQAEDGSWWTGYAFEDDALWPDERPTWTAGAVLLAADAMHGLTPASALFTDNAL
                     PDAPEHSERAGRFQRLEQARG"
     gene            complement(15848..16609)
                     /locus_tag="AC16QF_02170"
     CDS             complement(15848..16609)
                     /locus_tag="AC16QF_02170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6447388.1"
                     /translation="MSLETVDFARLKLAPGDNVLDLGCGEGRHAITAYLREAVTVVGM
                     DLSLGDLTTARQRYGEFRDGDDASRRVDFILATGHNLPFADATFDKVICAEVLEHIHD
                     YENVLGEIHRVLKPGGTLAVSVPRFGPEWLCWRLSDDYHQVEGGHVRIFRSRELRRSI
                     EACAPEPAGARPVAGKTQLSFQSRHFAHGLHSPYWWLRCLFWRRGESVWPVRLYHRFL
                     VWDLMRKPLLTRVLERALNPLIGKSVVMYFHRGAV"
     gene            complement(16606..17880)
                     /locus_tag="AC16QF_02175"
     CDS             complement(16606..17880)
                     /locus_tag="AC16QF_02175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6447389.1"
                     /translation="MSELATYEFGQEAPSGRRPLRIALLGYRSNPFSGGQGVYLKFLS
                     RALVDAGHEVDVISGEPYPELDGRVGLIKLPGLNLFDVPNHVTALRPKHLLSATDFFE
                     WLSMLSGGFPEPYTFGRRLTRYFSRIDDRYDVVHDNQSLCYGLLNVARRTPVICTIHH
                     PITWDRDIAIANAATRGERLLIRRWHSFLAMQTRVAKRLPNIVTVSERSKRDICAAFE
                     IPQGRVTVVRNGIDTEAFRPDPTVTRKPWQLVTTASADQPLKGAQHLLPAFAQLCRRF
                     PELRLVFIGAPRPGGKTDRLVDALGVRHRMDFIHGISTADIRRLYAESTVAVVPSEYE
                     GFGLPAGEAMACGTPLVSTDGGALPEVVGQAGRIVPSRDPDALAEAIAGLLVDPKERE
                     ALSRAGRARMVEHFAWAEAAERLTALYEQVLA"
     gene            complement(17960..18871)
                     /locus_tag="AC16QF_02180"
     CDS             complement(17960..18871)
                     /locus_tag="AC16QF_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447390.1"
                     /translation="MFNPNPIVVEAFAAHCTNAYVEAFANTLAQPREDHLETLREAVS
                     TALGLLARCDCPYHNVEHTIMVADAGLAILKGQLALEDPISPDDWLHAVVAMLFHDVG
                     YLRDLLPEDGPEGCLVEHGGRRVSPPADATDAWMMPYHVTRGDLFVRQHYADHPLIDV
                     DRVAAYIEMTRFPVPDRPPYQQLETLGALVRAADLIGQMGDPNYLAKLSSLFVEFKEN
                     GEADRLGFASVAELRADSQAFFSQQISPYLPKALKLLRATEEGRAWVVSLYENIERRA
                     QAGGSAAQPDPSKQENSRAGRIHAGNG"
     gene            19034..19960
                     /locus_tag="AC16QF_02185"
     CDS             19034..19960
                     /locus_tag="AC16QF_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6447391.1"
                     /translation="MSHLELRHLRTLVALRDTGSLVEAAERVFLTQSALSHQLKDLES
                     RLGVTLFIRKTRPVRFTTAGRRLLKLADQVLPLVHGAEHDLERLAGGESGRIYMAIEC
                     HSCFEWLLPAINAYREEWPDVELDIASGFHFAPLPALARGDLDLVITADPIEELGLVY
                     LPLFSYEAQLAVARDHELADKPWVEPADLSREVLICYPVDRSRLDIFTSFLDPANVEP
                     ARVRNAELTTMMIQLVASGRGVACLPNWALHEYRQRDYVQVKSLGEDGVWPTLYAAVR
                     ADQADAAFIRGFIDVAKQTCFQNLVGIVTADV"
     gene            complement(19993..20568)
                     /locus_tag="AC16QF_02190"
     CDS             complement(19993..20568)
                     /locus_tag="AC16QF_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6447392.1"
                     /translation="MDPLAQLTRDRAEARRLEDPCANLCTVASVDSAGNPQARTLVLR
                     DVNERLAIFANETSPKWREISLNERISVVVWLPVLNLQYRLRCGTERVPTHLVHDRWH
                     QRPDPPKRLDWFYTNHLPQSSPLPDRDTLLERLAQIDLPEPLAPPQTAAGLYLSPTTV
                     ERLDLNQSNGIHDRRHFELSEDGWVQSVLVP"
     gene            complement(20618..20809)
                     /locus_tag="AC16QF_02195"
     CDS             complement(20618..20809)
                     /locus_tag="AC16QF_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447393.1"
                     /translation="MVFLCFIVLLGIAYLVWRVTDQMPDLIFRLSEVQRDVAEIRRRL
                     TEESDAPAGAAQGGGDAEA"
     gene            complement(21047..21541)
                     /locus_tag="AC16QF_02200"
     CDS             complement(21047..21541)
                     /locus_tag="AC16QF_02200"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SixA phosphatase family protein"
                     /protein_id="HIR6447394.1"
                     /translation="MKIWIIRHAKSSWAQPGLSDFDRPLNDRGRADGPRMAAWLASQD
                     APAEWIWTSDAARARATAAFVERGFSAARPLVVEEHALYHASARGALDVLRGTPEAVA
                     SVALVAHNPGLTDLVNLLAGNLVTENLPTFGVARFDVAAPWHSVGEQRADLEILTAPK
                     EIPS"
     gene            complement(21553..21915)
                     /locus_tag="AC16QF_02205"
     CDS             complement(21553..21915)
                     /locus_tag="AC16QF_02205"
                     /inference="COORDINATES: protein motif:HMM:NF025178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR6447395.1"
                     /translation="MSEAKQINLGPLSDLEEGDKRSFDAEGTQVLVCRVRGELYAIED
                     LCSHANKPLCRGRLSGYMITCPVHSARFDIRDGAHQGPPAFTGIRTFPIEEGADGAIV
                     TVTPKPLPAGFGGPLMTR"
     gene            complement(21924..23258)
                     /locus_tag="AC16QF_02210"
     CDS             complement(21924..23258)
                     /locus_tag="AC16QF_02210"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6447396.1"
                     /translation="MSSIRQMRGIDRTKATEPELGSNPINGDRYRSREFAELEWEHVW
                     TRVWLIGGLVHQIPEPGDFFTTDIGRESILFVRGDDGSIRAFYNVCQHRGNQLVQAEQ
                     GSLSGGEFQCAYHGWRFDAQGVLTWVYCEEDFPQGNPCGKRNLIEIPCATWAGFIWYN
                     MDPDCKPLADFLDPVARQLDNYRMEDMRRTHWVTLEGNFNWKCVQDNFNESYHLPYVH
                     PQSCISLDEHYSSCQFDLYPSGHCRMFMPGGGPNSNYQGSPEAVFERLGQDLAFWEID
                     PEPFRDDLAGLRTALQQQKRKLAQKKGYDFSRYSDEQLTDNYHYTLFPNLSFSMKPDG
                     CIFLRGNPHPTDPEKCLFDMWYLTLFPEGAKEYYSNSMRDWVSVETPAAHERGKVGEV
                     SCGPGIDQDVAIWSTQQKGLTSRGFRGEYMPTQERRIRFFHENIDRYINGQL"
     gene            complement(23361..24269)
                     /locus_tag="AC16QF_02215"
     CDS             complement(23361..24269)
                     /locus_tag="AC16QF_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6447397.1"
                     /translation="MSDRRPAVSLAAVPGRRNTIVELAAEIERRGFAGIYCPSMGDGL
                     ALCEAIAYNTRQIEFGTSITPIYTRHANDFASTAAFIHEISNGRFRFGLGVSHAPAME
                     RLGVRQGKPLSDMRQFVSDLRAAPRVGELPPVVLATLRDRMIGLAAEVAEGMVFANAA
                     RGHMQHSLNVLGDERRADPGFFIGNMIPTCISDDVEAAKAVNRRTLTGYAMLPNYRNY
                     WKEAGFVEEMGAVEKALEAGEPDRIPDCLSDAWLAETTLFGPANAVRDGIEAWFDAGV
                     RTPIIVPSSAVGNQLKAFEEFFEIWS"
     gene            complement(24378..25139)
                     /locus_tag="AC16QF_02220"
     CDS             complement(24378..25139)
                     /locus_tag="AC16QF_02220"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR6447398.1"
                     /translation="MFPLRDENPRVHPPYATVAIIILNVLVWVAVQGFGNHYALAESL
                     CLYGLIPGDLLGNAVPGTSIPLGSNLVCQLDGTANPASLFTSMFMHGGWFHIIGNMWF
                     LWVFGDNVEDVMGPFRFVVFYVVCGLAAAFAQIATNTSSLVPMVGASGAIGGVMGAYA
                     LMFPRAHVHTLIFLGFYVTTVAVPAVFMLGYWFLLQLLQGLPAMGSSQGGVAFWAHIG
                     GFLAGVALIHLFRRPNHLAQHLAQPERRSSRHRWF"
     gene            complement(25259..26560)
                     /locus_tag="AC16QF_02225"
     CDS             complement(25259..26560)
                     /locus_tag="AC16QF_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR6447399.1"
                     /translation="MTPKAGSYFPSQAASTLYAMAGDDCSLHFDLNTLTEKQLADHVS
                     NIELTAMLQVALLYVGKPVDHVALFDEFQRPPWRETLDLYRRAFTEMGVPPVPESNCH
                     YYPRDGFYEAVAADRVDADLTTLYLVSGSNAVVHRSDAALSVSQRVNSKMQLAADAAQ
                     WGIPVPETLVCRKRDLDGPEVSGFFDTHHNQIMLKLKGLAGARNVTSVAGVPECMDYV
                     AEYPNDMEIVLQRKLSADEFTEMTVDLIVRDDSIEIANVRRILFADGLWVGNLIGGGV
                     TVDPAHERALLRVGEYVRDLGYRSPEGFNCGIDYFVSADELLVTEINARWTGGLFPAE
                     MIRRLDLAGRGAVAFFDMVSRDGLPRYLDFSEQTLYRSQDEPFASVPLGFSPFPMPVD
                     GRDMHPVWQMVVGDIEAFKAAKREALEPGDLPTADKISLTF"
     gene            complement(26655..27743)
                     /gene="hisC"
                     /locus_tag="AC16QF_02230"
     CDS             complement(26655..27743)
                     /gene="hisC"
                     /locus_tag="AC16QF_02230"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR6447400.1"
                     /translation="MTHERQNIRAMHGYTWGEQPERADVIKLNTNENPYPPSPAVDRA
                     LADLSGEALRAYPQPTADRLRDQIAELHNLKRENIVVTNGGDEGLRLAFTTFLEPGGT
                     FGMADPSYSLYPVLAAIQDTRVLSIALDENWDLPRDFAHQLNDAGAGLACLVNPHAPS
                     GSLLDTSRISQLANDFNGVLLIDEAYVDFVDPALRYDAVRLTEAFDNLLILRTFSKGY
                     SLAGMRLGYLVGCASLIDPVIGKTRDSYNVDYVSQRVGEAALSDPAYAEETWANVRAG
                     RRTLREGLLEHGFRVGASQTNFLLTEVPAASPCSAKQLYEALKTRGVLVRYFDTPSLR
                     DTLRITVGTPEQNQTLLTWVGDILSRGE"
     gene            complement(27740..28786)
                     /locus_tag="AC16QF_02235"
     CDS             complement(27740..28786)
                     /locus_tag="AC16QF_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR6447401.1"
                     /translation="MGSTSKTLIVVATLIVIAAGIKAAEALMVPFLLAAFIATIAATP
                     VFWLERRRVPAGIAIALVMLGIIVMITGVGAVVAQSAGAFTNRLPFYQDRLTELVENA
                     VTYVEMAGVNISEEALLSYFDPGTALVMAGNTLRGLGGALSNGFLILLTVIFILAEAS
                     SFPRKLQEVLDDPDSDLPHFVRFAENVNRYIGIKTTISIITGVLVGLMLAVLGVDFPA
                     LWGLLAFLLNYVPTIGSLIAAIPAVLLALIQLGPLTAAVAGAGFFVINLLMGNVVEPR
                     YMGRGLGLSTLVVFVSLVFWGWMLGPVGMLLSVPLTMTAKIAMEANPSSQWLAVLLGP
                     AERAADPTPAEDQT"
     gene            complement(28813..29658)
                     /locus_tag="AC16QF_02240"
     CDS             complement(28813..29658)
                     /locus_tag="AC16QF_02240"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR6447402.1"
                     /translation="MNQGHRLTRVGVVQLCATPDVTANMIAVERLVPQACDAGAEVVL
                     LPEAFAFLGPEKAKRGILERLPDAPSAPGGPILEQCRSMAARLGCHLILGGFHEDAGD
                     PDKSYNTCVHLSPGGELLARYRKVHLFDVALADGTQLRESARTLAGHDLVTTDMPFGK
                     LGLSICYDLRFPYLYQSLVDAGAVALTVPSAFTKTTGAAHWHVLLRARAIECQSYVIA
                     PAQHGHNWGKRFSYGHSLIVDPWGDIVAECRDGDGVAVADIDPERVRRVREELPSLTH
                     RRLLE"
     gene            complement(29701..30891)
                     /locus_tag="AC16QF_02245"
     CDS             complement(29701..30891)
                     /locus_tag="AC16QF_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009867821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6447403.1"
                     /translation="MSKWRIEDFPVAPTEGKDRFHDFALPDPLMHAIADLEFEYCTPI
                     QSAVLPWSLADYDVTGQAQTGTGKTAAFLVTIIARLWEYPPAAAPKIGSPRSLVLAPT
                     RELALQIESDAKDLTKYIDLGVECVVGGMDFQKQLDRIKRRPVDVLIATPGRLLDFIS
                     RRHLSLRDVEVLVIDEADRMLDMGFVPDVRRIVYQTPHKRNRQTLFFSATFNDDVMRL
                     ANSWTIDPEHVAITPESVATDTVTQKFWLVAADKKASLLAEFLTRHAPERALIFTNRR
                     DQTHQLHAFLNKRNIRCEALAGDVPQRKRLSTLSRFKDGDIPYVVATDVAGRGIHVEA
                     ISHVINFDLPEDPEDYVHRIGRTGRAGAEGVSISFVSEDDAFNLPSIEEYLGEAVVCT
                     QPSF"
     gene            complement(30884..32203)
                     /locus_tag="AC16QF_02250"
     CDS             complement(30884..32203)
                     /locus_tag="AC16QF_02250"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF024301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004069 - L-aspartate:2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase type Ic"
                     /protein_id="HIR6447404.1"
                     /translation="MTTARAIAALSDPELAALKAELSRELELQAGNRLSLDLTRGKPA
                     ADQLDLSAGLDDAIAGNYIASDGTDSRNYGALTGLGEARELGAEIMEADPGDIICWGN
                     SSLQLMHICVDLALNQGLWGDGRRWRRDANPKVLTPVPGYDRHFTICEAAGIDMVNVP
                     MTDTGPDMDAVRRATEDASVKGIWCVPKYSNPSGCIYSPECVDALARLPADAAADDFL
                     ILWDNAYAVHDFEFPRAPLASLLDLARKHGTADHVIMFASTSKITYPSAGLAFLAADA
                     KVRKTVEKRLGTASIGPDKVNQLRHARFLGGRVEAHMAGHAALVKPKFDLVESKLREG
                     LAGLEIASWTTPAGGYFVSLDTRPGLAKEVGELAAQAGLTITKPGATFPYGRDPEDRN
                     LRIAPTFASLADLNVAMELLVVCVKLAAVGAEIHQRTDTGGAQKAHE"
     gene            complement(32200..33147)
                     /gene="argC"
                     /locus_tag="AC16QF_02255"
     CDS             complement(32200..33147)
                     /gene="argC"
                     /locus_tag="AC16QF_02255"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR6447405.1"
                     /translation="MIRVFIDGQAGTTGLEIVQRLAARDDVELLEIDPAERKSPAARQ
                     RLFDSADVAVLCLPDDAAREAAVLADGRCRIIDASTAHRIQEGWCYGLPELGSNQRQA
                     LAAAERVANPGCYPQGFILMIRPLIGAGLLDPEQPLAVQALSGYSGGGRTLIEARRAF
                     GSQEAERRNTEPYALSLKHKHVPEMQAYSGTKIAPLFTPSVGQFYQGMLVGVPLFCSQ
                     LRKGADYDGIYHILEEAYALEPFVDVLEPGAETALTDGFLNATACNGTNRLEIMLFGH
                     SEQLLLMARYDNLGKGAAGAAVQNLNLMMGIDETTGLTS"
     gene            complement(33299..34075)
                     /locus_tag="AC16QF_02260"
     CDS             complement(33299..34075)
                     /locus_tag="AC16QF_02260"
                     /inference="COORDINATES: protein motif:HMM:NF018649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIR6447406.1"
                     /translation="MGLFELYGVAALTVWVMVTTLWAVSVPTKDASIIDMFWGPLFVA
                     IAWVLLPVYGGGVQAKPYLVMLLVTLWGLRLAYHLIGRNLAAGEDVRYARWRASGGPK
                     WWLRTYHRIFLLQGFLALLVATPILASFYAPRDLTLLNLAGVLIWAAGFVFELVADVQ
                     LSRFKADPANRGQLLNTGLWGLSRHPNYFGDALQWWGLSIVALSGAAWWALLGPVVMT
                     MIFLVLTADILEQGMSRRHPDYEEYVSNTPKFVPRLPGRD"
     gene            complement(34173..34826)
                     /locus_tag="AC16QF_02265"
     CDS             complement(34173..34826)
                     /locus_tag="AC16QF_02265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6447407.1"
                     /translation="MTDLLDCVEIDPGGTAGSVIWLHGLGADAHDFEPVVPLLPLNRP
                     LRFVFPNAPVRPVTINAGMEMRAWYDIDPGNPLSGDSDIQSSAKAIAGLVEREHDRGV
                     PYDGITLAGFSQGGVIALELGLSFPHRLAGIMALSTYVPDHEHLVDRIGFANVDAPIF
                     MAHGIGDPMIPITRAITSREALLAMNYEVEWHEYGMGHQVCPEEIADIGTWLNTIYR"
     gene            34960..36789
                     /locus_tag="AC16QF_02270"
     CDS             34960..36789
                     /locus_tag="AC16QF_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009686258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR6447408.1"
                     /translation="MEDGLDRQASRELTRLLPALGFLRPYVRQVVFASIALVVTAGVT
                     LSIGQGMRLVIDEGLGSGSAAVLVQSISIFAVLMVVLTAGTFLRFYFVSWVGERVSAD
                     IRQAVFAHLIRLHPGFFETNLPTEIQSRITTDTTVLQTVIGSSVSIALRNVLMFVGGL
                     VLLFVTNPKLSLIVVASVPFVVAPVILFGRRVRALSRDSQDTLAEVGSFAGESLRNIK
                     IVQSFNHEGLDIEAFRQRVERAFQVAVRRILQRSWLVALVMMLVLGAIATMLWVGGRD
                     VLAGQTSPGELAAFIFYAFIVAGSVGAISEVYSDLQRAAGAMERLVELLNSPSLLTDP
                     ESPVLPAAREPSVAVSPGGRLTVRNLGFAYPSRPDAPVLRGLSFAVAPGEMVALVGPS
                     GVGKSTLFDLLQRFYDPDAGQILLDGVDLRELRLADVRERVGLVPQDPVLFAGTVESN
                     LIYGAPDAGDDEKSRALELAHASAFIEALPEGLATVVGEGGVGLSGGQKQRLAIARAL
                     LKRPEILLLDEATSALDAESEQHIRASITELKGQCSIVVIAHRLSTVREADRILVLSE
                     GRLQASGRHEELLKSSELYARFSRIQFAGEDEADGSGARTTAG"
     gene            36857..37990
                     /locus_tag="AC16QF_02275"
     CDS             36857..37990
                     /locus_tag="AC16QF_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6447409.1"
                     /translation="MGPLHGFRIVELAGIGPGPFCGMMLSDMGAEVIRVDRISGGANR
                     PKDVLARNRRSIAVDLKSPAGAETVLRIVEKSDALFEGFRPGVTERLGLGPEDCWARN
                     EKLVYGRMTGWGQEGPLAQAAGHDINYIGLSGALHAIGHAGDRPVPPLNLVGDFGGGG
                     MLLAYGLVCGLLEAQKSGKGQVVDAAMVDGAAALMAMFFSMGAAGVFKDQRGTNLLDS
                     GSHFYDTYETKDGKYVCIGSIEPQFYALLVEKAGLDAERFAAQMDASRWPDYKRELTE
                     VFKSKTRDEWCEIMEGSDVCFAPVLSIFEAPDHPHSQARNAFIELDGVVQPAPAPRFS
                     RTEVAASHGARVPGEDSVSVLEDCGFTGDEIAGLKNDGVIGGN"
     gene            38006..38542
                     /locus_tag="AC16QF_02280"
     CDS             38006..38542
                     /locus_tag="AC16QF_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447410.1"
                     /translation="MAVELLDTERFHGFRVRRQIAGKTYQEYFSLKDDGKRIKGPQRA
                     TVKALAEARDAELANLQKAARERADRTVRVDARGQVRGILCRLKTEKSGTLTPVFQIG
                     VMSRLEDKIVNTTVSISRYGETEAWRRAIDFYALHKGISKRSRAYRDLLAAQPSGAKI
                     KSLLKASGRAAPRRSARR"
     gene            38539..40425
                     /locus_tag="AC16QF_02285"
     CDS             38539..40425
                     /locus_tag="AC16QF_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016562180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6447411.1"
                     /translation="MSYASEYARSLSDPEGFWREKAAELPWLEFPQTILDRAPSGAWR
                     WFHGGKTNTCWLALDRHVADGRGAQVALIYDSPATGSVQRFTFAELTERVAACAGGLA
                     ALGVSRGDRVIVYMPMIPEAAIAMLACARIGAIHSVVFGGFAAPELATRIDDAEPKVV
                     LSASCGIEFANVIPYQPLVDEAISLAVHKPERVVMLQREQQEADLSGERYLDWASWEA
                     SAQGVGCVPLDATDPLYVLYTSGTTGKPKGVVRDNGGHAVALNYSMEAIYGMDAGDVF
                     WAASDVGWVVGHSYIVYGPLIRGCTSVLFEGKPVRTPDAGVFWRVIQDHRVKCFFVAP
                     TAFRAIRKEDPDCALKDRYDVSSLKYQFVAGERLDPPTYHWLSEHLHVPVIDHWWQTE
                     TGWAMCANMAGLELKDAKPGSPTLPVPGYDLRILDEDGVEVPAGVEGAVVVREPLPPG
                     AFPTVWGDHQRYVDSYWSRYPGFYLTGDEGYRDDEGYVYIMGRMDDVINVAGHRLSTG
                     QMEETVAEHPAVAECAVVGIADADKGQVPVGLVLVKDGVNVDPQALEAELVQMVRASV
                     GAFANFRRAVVVERLPKTRSGKILRQVLRKMIDGNPYSVPSTIDDPAIIPEMASTLKK
                     AGVM"
     gene            40457..40792
                     /locus_tag="AC16QF_02290"
     CDS             40457..40792
                     /locus_tag="AC16QF_02290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6447412.1"
                     /translation="MIIVHGTIPIRPDQRERALELARRMSEATQAELGCISYDFYVGL
                     SDPNTLMLFQEWETMEALMAHFQTEHMDDFLRELPQVVAGEITTRRYAVQSVDEDTEE
                     TPETPPIVH"
     gene            complement(40786..41136)
                     /locus_tag="AC16QF_02295"
     CDS             complement(40786..41136)
                     /locus_tag="AC16QF_02295"
                     /inference="COORDINATES: protein motif:HMM:NF021369.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HIR6447413.1"
                     /translation="MKGLQLACLLMMGSLFAINGLRQFFVEPLATPHVNLIWFLIQVL
                     PLLAVLPGTLRNRYRSYVFAALVSTLYFIHGVLLAATADLRALGLWETGFAVGLLIAA
                     SYAARRTVGQAASQ"
BASE COUNT         6971 a        12979 c        14038 g         7275 t
ORIGIN      
        1 ccgcatagaa gggtgccgac atgccggcat cgatgaagcc gggacagacc gaattggcgc
       61 gaatgccccg ctcggcgagc tccacggcca gctggcgcat cagctggcgg acccctgcct
      121 tggccgcggg ataagctcca gagccggggg ccggcgtccg gtcgttgatg gaggtgatgc
      181 tgatgatgtg atccccgccg ctcatccgtt gggcgcactg ccggctggcc acgtagcagc
      241 cgatcaggtt gacggatatc accttgacga agtcggccac gctttcctcg accaccggcc
      301 cgaagtgcac gatgccggcg ttgttcacca gaaggttggg cggctcgccg aaggcgtcca
      361 gcgcccggtc gatgctgtcg gcgtcggtca cgtcggcggt cagcggcagc gcatcgggca
      421 gcgcggccgc cacctccgcg gccgccgtgc cgtccatgtc gagcacgccg acccggtatc
      481 cggcgcttcc cagccgggtg gcaatggcgg cgccgatgcc gtgggcagcg ccggtcacca
      541 gcgctcggcg cacgctcatg tttgccaccg cacgcggacg ggaaagcggc cccagttggg
      601 acgcgaggtg tcttcgtcgg gcaccggcag gtcggcagga tcgaacatgg cgtcgagctc
      661 ggatagaaag tgatgaacca ggagcgtcac cgtgccgtac tgatggctgc cggcttcggt
      721 atcggaatct tgcggcaggc cggcgtctat gtctcggccg aggcagttgt gcacgccgat
      781 cccgaaggtc agaccgaagg ggttggcgcg ccggtccagc accgcgcgcc cgggctggaa
      841 cgcgtccgcc tcgggcccga aaacctccgg gtcccgattg gccgcggcga tgtccaccac
      901 caggtgatcc tccggctcca cgtgcaggcc ccagctgaaa tcgatgggct cgagcgcccg
      961 gcgccaggcc acggggctgg cggggtgaag ccgcagcgcc tcgtgcacgg cccgctgggt
     1021 ggcgagcgca tcgccggcca gggccgccct gcgggcgagg tcgctgccga gccaggtcag
     1081 cacctcccag agcgcgtggg cgaaggcgtt ggacgtgctg tgggcgccgg cctgaaggta
     1141 gaaggcgatc tcccgcgcga gaccgtcgtc gtcgaggccg ccccggtccg tcagcagcac
     1201 cgtgagcatg tcgcgaggca gggattcctc gggtatctcg ctcccacgcc acgccgagat
     1261 tagccgctct cgccgcgcca tggacggctc caggaagcgg ccgcggtact cggccagcgc
     1321 ttcgcgtacc tctgccctga cctgatcgtg ggcgcgcgtg gaatgcacca gggtcgctcc
     1381 ctcgccgaat ttcttcgtca accgctgcaa cgcggccgtg gcttccgggt cctcggaccg
     1441 gtcgatgccg gcgaaatcgg ctgtcaggtt catggatacg gcgtggccga acgccacgac
     1501 gtcgatgctg ggctggcccc gcagcggctc cagggcgtcg cgcagcgctt ttggaaaaac
     1561 ctcgcgctcg tagtgacggg cgtagtctct gcggaagacc ctcagggcga ggtgccgtcg
     1621 gcgggtgtgg cgctggccgt gcagggtcag cagcgtgtct tccatgatca ccccgccggc
     1681 gtcgtaaagg gactgtttca gatgcgggct gcacaacgcc tggtgagcgt ggcggtagtc
     1741 ggtgatgcgc aggtccatct cagcccaggg tgacagggta ggtcgcccag atgtcgcgcg
     1801 cggtggtggt gtccttgcgc ggcggcgagc cgggctcagg ccgtgcccga tgggccagca
     1861 gggtgcgcac gatcatcgcg acggtgccgt agtggtgatc gtcgggatcg gtgtccggct
     1921 tcggcagcgc gccggcggcc agattgagcc caaggcaggc gtgcatgccc aatccgaagg
     1981 aaagtccgta ggggggcacc tttgccccgc ggggtcgatc agggttgaac cgctccgcat
     2041 ccgccccgaa tgctgccggg tcgcggtttg ccttctgcag gtcgacgttc acccgggtgt
     2101 ccgcctgaac gtccttgcct gccacatgga ccgggcagag cgcgcggcgg gcggcaatgg
     2161 ggctcgaggg atgcaggcgg atggattcgt ggacggcgcg ctgcacgaac agcgggtcgg
     2221 tctcgaagcg cgaccagtct tccgggtggc tggcgcacca cgtgaagatc tcgtgcatga
     2281 cgtggcctag ggtgtgaatc gaggtatggg cgcctgccag cgcgaagaaa gccatctccc
     2341 ttacccgcac gtcgtcggcc agctgcacgg tgtcctggtt cttcaggagc accgtgagga
     2401 tgtcccgggg caggtcctcc tcggacagat cgcctcggtt cacgctggcg atgagcgcct
     2461 ggcgacgagc caccgaaggg tcaacgtagt cgcgctgaaa atccgcgagc gcgtccagcg
     2521 cggcacggcg aatcggttcg cgatcctcgg tagagtgggc gagggtggcc gtgcggccga
     2581 acaacctcag catctttatg agctgctcgg tttcctccgg cgacttcttc ggccggtcga
     2641 tgccggtgaa gtccgcggtg aggttgacca tgacgcggta gccgaagtcc acgatgtcca
     2701 tctttccggc ggcgaggaag ggggcgatgg tctcctcgag ggtgcgcgga aaaacctcgt
     2761 gctcgtagta gcggaagaag tcgcgacgga agacctgggt ttccacgttg cgccgggtcc
     2821 tgtgctcgtc gccgtgcagg ttgacgagca cctcgcgcat gatcacggcg ccctcgtcgt
     2881 agagcgcctg acgcagatct ttctgtttca gcgccgcgtt ggcggcttgg tacgagtcga
     2941 cggtgaccag gctcatttat gtgcgtccgc ccagaaaagc tggcttgagg agtaggcgga
     3001 gacccgccag ccgggaatgg gcttgcgtgc gggcaggggg ggcaggtcca gctgcgcacc
     3061 gttcaagaac acccgttcga ttttcgtctt gtcgccgagg accgtcacgt ccttggacgg
     3121 gtcgccgtcg actaccagga ggtcggccag ccgcccggcc tccaccgcgc cgacgcgatc
     3181 ctgcaggccg atggcgcgac cgcactccga cgtggccgcg cgtatcgcct cgagcggggt
     3241 gaaacccatg tcgcggacga acacctccat ttcccggtag tgccactcgc cgtaaggcgt
     3301 cagcgagaaa ccggtttccg tgccgcacag cacgggcacg ccggcctcgt aggcgcggcg
     3361 gatcatgtcg gcgctctcgg cgatctcccg ccggaagatc tctcgcaggt tggggtccgc
     3421 ccccaccaga tcgccgaaat cagcgaggtt ggcctggaac gtgaaagtcg gcaccagcgg
     3481 gaccttccgc tcgatgaccg cctcgagcgc ggcgtcgtcc atgaacgtgg cgtgcaggat
     3541 catgtccatg cccgcccgga cggagtccgc gatgctcttc gcgttgcggc agtggccgac
     3601 cacgggtatg ccgagctcgt gggcggtgtc gcagacggtc ttcagctcgt cgaaggacca
     3661 ggcctgaatc tcaccgcggt gcttctgccg cggaaccagc cccgtcacgt gaaccttgat
     3721 ccagtccacg ccgagcttta tctggcggcg cacctcggtg acgatctcgt cgcggctgcg
     3781 cactaccttg gcgtagccga cgcggccctc cgccggaatc agattaccgg cggtgccgcc
     3841 cacggacgtc agcagagcgt tcccgccggt cgacatccgc ggcccgggca cgatgcccgc
     3901 ctcgatggca tccctcaggt ccacgccgat atcgaagatg cagtccgcgt cgaggaaacc
     3961 ggtgaccccg gcacggagca gcttctgcac gttgtacgcg gccaccaggg cgccgaggcc
     4021 ttcccggcgg tggaagaaca tctcgtcgtt cgaatgggtc tcgtccagcg tcacgtggca
     4081 gtgcgcgtcg atgaggcctg gcatgacggt cttgcccgcg acgtcgatga cggtcgcccc
     4141 ttccccgtcg gctgcgccca ccgccaagat gagctcttcg tcgatgagca cgtcgccggc
     4201 aaacggcgcg ttgccgagcc cgtcgatgat gttggcgttc ttcagcagtg tcttcacagc
     4261 gcctgctcct tgatcacccg ccagatcttc tcggacgtgg caggcatgtc aagctccctt
     4321 actccgagag cgtcggtgat tgcgttgatg acggccggtg gcgctccgat ggcgccggcc
     4381 tcgccgcagc ccttgatgcc gagcggattc gaaggcgagg gtgtattcgt gaacccgacc
     4441 cggatatccg gcaggtcgtc cgcgcgcggc atgcaatagt ccatgaagga cccggacagc
     4501 aactggccgc cgtcgtcgta gacggtgttc tccagcagcg cctggccgat tccctgagcg
     4561 accccgccgt gcacctggcc ttccacgatc atcgggttta ccagcgtgcc gaagtcgtcc
     4621 acggcgacga agtccacgat ctccaccacg ccggtctcac gatcgatctc cacctcgcag
     4681 acgtgacaac cggcgggaaa gttgaagttg ggcgggtcga agtagcaacc ctctttcagc
     4741 cccggctcga tctgtgtggt gtcgaagtga tgggccgtat acgcgttgag ggcgacttcc
     4801 gcgaaggaaa ggcgctgatt cgactggggg ctcgagaact ctccgtcagc gaagctcacg
     4861 ctctgcgttg gaacatccag gacctgggcg gcgatgagct tggctttggc aaccacctta
     4921 tcgcaggaga tggcgatggc ggacaggccc accggacccg aacgcgagcc gaaggtcccc
     4981 atcccgaact gcaccttgtc cgtatcgccg tggacgatgt ccacgtcctc tacggcgatg
     5041 ccgaaccgat cggccacgag ttgcgcatag gtcgtctcgt ggctctggcc gtggctgtgg
     5101 cagcccgtca gcacctcgac gctgccggtg ggattcaccc tcacctccgc cgactcccac
     5161 agccccgcgc ccgatcccag cttgcccagc ttggccgagg gaccgatccc ggctgcctcg
     5221 atgtaggcgc cgatgcccag gccgcgcagc ttgccctgct cttcggatgc agcccgccgg
     5281 gcaggaaaac catcacggtc gatgagctgc agcgcctggt ccagggtcgc ctggaagtcg
     5341 ccggtgtcgt actccatctc caccggggtg acgtaaggaa acgaccgcac gaagttgcgg
     5401 cgccgcagct cggcagaatc gatgccgagt tcccgtgccg cctcctccac cagccgctcg
     5461 accacgtagc ccgcctccgg gcggccggcg ccgcggtagg cgtccacggg tgccgtattc
     5521 gtgtagcggg cgatgacgtt ggcgtgaatt gccgggatct cgtactgccc cgacaacaag
     5581 ggggcgtaga cgtaggtcgg gatcagcgag ccgaacgtgg acaggtaggc gccgacattg
     5641 gcctgagtgt gcaccttcaa ggccagaaaa cggccgtcgg catccagggc gagctcggcg
     5701 gtggtgacgt gatcgcggcc gtgggcgtcg gtcagaaacg cctctgaccg atcggccgcc
     5761 cacttcacgg gcctgccggt ctcgcgggcc gcccaaaggc acgccacttc ttccgcgtag
     5821 ttgaatgctt tggatccgaa gccgccgccc acgtcctcgg agatgaccct gatcttgtgc
     5881 tcgggggcga agccgatcac ggctgccagc gtcattctca ggccgtgggg gttctggctg
     5941 gtaatgtgca gcgtgtgctg gccggttcca acatcgaact gcgcgttgag ggagcgcggc
     6001 tccatggcgt tcggcaccag gcggttgttt atcagccgca gccgggtgac gtgggcagcc
     6061 ctggcgaaag cggctgctac cgcgtcggca tcgcctattt cccagtcgaa cgcttcgttg
     6121 cctgcaatcc ccgaatgaag ctccggcgca tcgacggacc ctgccgaaac cacgtcggcg
     6181 accgccggca ggtcgtcgta gtcgacgtac acgcactcag cggcgtcccg tgcggcttga
     6241 agcgagtcgg caaccaccag ggcgaccgcg tcgccgacgt agcggacctt gtccgacgcc
     6301 agcgccgggt gctcgcctac cttcatgggc tcgccgtcgc gggagtggat catccagccg
     6361 cacatcagcg ggccgagacc gctggccgcg aattcctcgc ccgtcagcac ggcgaccacg
     6421 ccgggcatct ccctggccgc ttccgcgtcg atggaattga tagcagcgtg agcgtggggg
     6481 gatcgcacga aggcggcgaa ggcctgatcg ggcaggagca ggtcgtcggt gtagttcccg
     6541 cgcccggtga tcaggcgccg gtcttccttg cgcttcaccg acgcgccgaa cgcccgcgga
     6601 agggcgctcg gaacggcttg ctcatcgggt cgcgataccc cgcccgccgg gccgtgcggc
     6661 tccttcgcgg tgctcatctt tggctctccc cacccgcaaa tcctggtatg ccgcggcgac
     6721 gccgcccacg gaccgggccg ccggcaagct taccgcatcg tgccaatcgt gggtaaaggg
     6781 ccagggcgct tggcaggggg cagccgcagg tcacggcgcc acgtcgaaga gctcgcggaa
     6841 gccgtagggt tcgtaggcgc cgatgacgag ttgctccagc acgcctttgc cgcgctccga
     6901 gccgcgggca tccgtgagct gcgcatcgca gattgcctgg acgtgaaaat agccgggatc
     6961 tgcatcggct atctcggcgg tctcgtagac ctcgtaattc gtcgccagct cgccgtggaa
     7021 cagcccgtgg ccccactccg gatgcatgta gcccagcccg cgcatgtaga aggtctgggt
     7081 cggctgcagc tcaatggtcg cctcctcgcc gttttcccgc acgtactcga gggtggcgct
     7141 ctcggccagc cgggtgccgg accggtgccg gatgctgctc gctacggccg ccatctcctc
     7201 gctgcgctcc ggatcggcca ggcggcccat ggtgttccag ggtcgacctt cgcgatccgc
     7261 gttgaggtgg taatagttgt tggtgctcgg gaagttcagg ggcgcccaca gccagtagaa
     7321 ctggggctca cccgcgtacg ggttgggctg ggggtcgcgt gcgcccaccg agcggatgcc
     7381 ccaggaccgg tcccgggttc cccaggtctc gtcgtgggtg agctcgtaac gctggccgtc
     7441 gacctcgagc cagccctcat agctgccgtt ctgggtgagc cgggtgtagt ccatgaaggt
     7501 gaggctaccc tgacggcggg tgaagcgcgg ttcttccacg gcggggaccc gggcacgaaa
     7561 acagacgtcc gccgccacgc cgttggcggg atccgcgcag ctcaccctca ggacgttgag
     7621 cggctcgatg acctcgacct cgatgggacc cacctgggtg ttcagccgct ccatgtggag
     7681 cactttggac gcctgcacgt tgtattgcct gccgccgacc agaatgctga aggcggcatc
     7741 catgacgttc aggtgcggat agacgccaag cgccgcagcg aagaacagct caccctcgcg
     7801 ctggtacccg ttgaagaaat agcgatcgta gaagttgcga tccgtcccgg aataagcgat
     7861 gggctccggg gtctggtgga tgggatagtc gtccgctttt gttaacatcg acctcttcca
     7921 ggggggcgga aaatcaggtt actcgtggac tgggagcgct acaagcagct gtgcgatcag
     7981 cccaacgtct tttcccgatg gatgctcaac cagaccatcg agctgatcgg agagagtccg
     8041 gtcgccgata ccctggcagg ggtgttgggc tcgtttcccc tggagaagcc ggacgatcac
     8101 cgcggcggac ccgcgacgga catgttcgtt ctggaactcg aatcaggtgt ccggcgtgcc
     8161 gtggtggagc tggtcctcct ggctcgggaa gcgggcgccg caaccgaagc gacctcgcat
     8221 cgcgggttgg gcggtttcgt cgaggcctgg agggagtatc tgaactggac gcggggctga
     8281 ccgccagttg tgctgcctcg cgcccgacgg tgtgccgctc cggcgagatc ggtaaaagag
     8341 ataaacaggg agaacgacgt gagtgatgac aaggcagtag tgctcgagct gatcgatggt
     8401 ttcaacagca ttgatctgga ccgcatcgtg gcgtgcttca ccgaagatgc tgtgtacgac
     8461 aacattccca tgcaggccgt gcagggcacc gaggcaattc gggcgtcgct ggaggggttc
     8521 atgggcgcgg ccagcgaggt tcagtgggac ctgctcaacg tggtcggtga gggtggcgtg
     8581 gtgctcaccg agcggatcga caaattcaag gtcaacggca cctggatcga gctcccggtg
     8641 atgggcacct tcgaagtcgc cgacggcaag atcaaagcct ggcgcgacta cttcgatctg
     8701 gctcagttcc agacccagtt cgccaaggcg acgggcgctc agtagccgga cgagccgcgc
     8761 cgtgttccgg cgattgctga ttgccaaccg gggggccatc gcccggcggg tggtgcgtgc
     8821 ctgcaacgat ctggggatcg agagcgttgc cgtttactcc gaagcggacg cccatgcgcc
     8881 gtacctgtcc gaggcttctc gggcggtggg cctgccaggg gtgagcgccg gggaaacgta
     8941 cctcaacacg gccgccctcc tggaagccgt gcgcgcaagc ggcgcggacg ccgtgcatcc
     9001 cgggtacggc tttctggcgg agaatgctga ctttgcccgc gcggtggcgg ctgcgggagc
     9061 cacgttcgtc gggccggatc ctaagtggct cgaggtgatg ggagacaagg tggcggcccg
     9121 tcgcctgctc gccgaccacg gttttcccgt cttcccgggc agtggcggtc tcagcagtct
     9181 cgacgaggct ctgagcgccg ccgccgagct gtcatacccc gtgctgctca aaccggccgc
     9241 cggaggcggc ggcatcggca tgcggctggt tcgcgacgag gcggagctgg ctagggcttt
     9301 cgcatctgca caacggctgg ccgagcaggc gttcgggcag ggaggcatct acctcgagcg
     9361 ggccatcgac aagccgcgtc acgtggagtt tcagattctc gccgatggtg ctggtggggc
     9421 gttgcacctc tacgaacgcg aatgttccat tcaacggcga caccagaaga tcatcgagga
     9481 agcgcccgcc gtcggcctgc cgcgcgagga gctggatgcg ctcgccgcgc gcgcggaagc
     9541 ggtgatggca gagatcggct acgacaacgt cgggaccctg gagacgctgt gcacgcagca
     9601 gggcgagttc ggcttcctcg agatgaacgc acgcatccag gtggagcatg cggtgaccga
     9661 ggcggttacg gggctcgatc tggtggggct gcagatccgc ctggccgccg gcgcagcgct
     9721 tcccgagcgc cccgggctct gcggccatgc cgtcgaagcg agaatttacg cggaagatcc
     9781 gcgcacgctc ctgccggaca cgggcgttct gcgcgttttc cgggcgccgc ggatgcaccg
     9841 ggtacgggtg gagacgggtt accaggaggg tcaggccgtt acgccgttct acgatgccat
     9901 gctggcaaag gtcatcggct ggggcgagac ccgcgagctt gccatcggcc gaacgctggt
     9961 tgggctcaag ggattcgaga tccggggggt caagacaaac ataccgctgc tgctcgacgt
    10021 gctgcaggac gacgggtttc ttgccgggct tatccacacc ggatttctcg atgggcttgt
    10081 gcttggctcc gatctgcgga gcgggagcgg agtcggccgg aacgcatgag gaggtaacgt
    10141 ggctcgctac gaggtcgaaa gtgaggttca gggtaacgtc tggaagatcg aggtcggcga
    10201 aggcgaccgg gtgtcggcgg gagacgtgct gatcattctg gagtccatga aaatggagat
    10261 tccggtggag gcgcctgtct ccggcgtcgt cgcggagctg cgcgtgcgcg aggaggaggc
    10321 tgttgaagag gaacagattc tgctggtgct cgaggcggac tagggcggac gagcaattgt
    10381 gttaagttag agggccgtct tctgggttgg ggggaaacta tgtcggaact ggtcaccatc
    10441 gacgtatcgg gcggcgtggc cgacgtgcgt ctcaaccggc cggaaaagta caacgcgctg
    10501 agcccgcaga tgttcgatgc catcgtcgca gccggagagc ggctggcaca ggctaaggac
    10561 gtgcgcgcgg tggttctgtc cggcaacggc cgcggtttct gtgccggtct cgacatggcc
    10621 agcttccagg gcatgaccga gaccagcccc cggtcatccg cgggcagcct gctgcagcaa
    10681 gacgaccgac ccgaaaaccg ggcccagcgg ccggctctcg tctggaagcg tctgccggtg
    10741 cccgtcatcg ccgccataca cggcgtcgcc tacggcggcg gatgtcagat cgcgctgggt
    10801 gctgacattc gcttcgcggc tcctgatgca aagatgtcca tcatggagat caaatggggc
    10861 ctcattccgg acatgagcat tacccagacc ctgcgtgacg ttctgcctct agatctcgcc
    10921 aaggagctga cctttaccgg gcgtgtgctc agcggagcgg aagcctcgca gctcgggctg
    10981 gtgactcacg tcgtggagga ccccctggcc gccgccctgg agctcgcccg tgagatcgcc
    11041 gggaagtcgc cccatgccgt tcgagccggg aagaagctgt tcgaggagag ctggcacgcg
    11101 gatgcgcgca cggggctgga gctggaggcg gccctgcaga cgggtctgat cggctcgccc
    11161 aaccaggtcg aagccatcaa ggcgaacttc gagaacagga agcccgattt cagggacgat
    11221 tgacggaacc gcacgatgag ctgggaaacc gaggttggtg agatcgaacg ccgccgccat
    11281 cttgcgcgcc agcagggcgg gccggagggc attgcacgtc agcacgccaa gggccggctc
    11341 accattcgtg aacggatcgg cgagcttctg gacgagggca gttttcgcga gcagggcaag
    11401 gcgacggcca tacccgacta cgacgagaac gacgagctga ccgggttcgt tccggccaac
    11461 tacgtggtgg gtttcggcca ggtcgaggga cgccgcgttg tggtgggcgg cgaggacttc
    11521 accctcaagg gcggctctcc gaacgcggcc ggattgcgca agagcgtcta cgccgagcac
    11581 cttgccagcg agtaccgcgt gccgctgatc cggctcctcg agggtggcgg gggcagcgtg
    11641 aagggatcgg gtcgtaaagg aggaaccgtc ggtgaacccg tgtacgccga cccgcgcttc
    11701 agaatcatcg ccgacacgat gaccgaggtt ccggtggcat cgggggccat gggggcggtc
    11761 gccggatttc cggccggccg gctggtggcg tcccacttct cggtgatgac ccggcacacg
    11821 gctcaggtgc tcatcggcgg tccggccctg gtcgagcggg cgctgggcgt gcgcctgtcc
    11881 aaggaagagc tcggcggcgt gcaggtccat gcgctgagcg gcgtggtgga caacatcgcg
    11941 gaggacgagc acgacgcctt tcgccagatc cgccgctttc tgagttacct gcccggtaat
    12001 gtctggcagc gcgctccgcg cgcgccgagc gacgacgatc cgacccgcat ggagcaggag
    12061 ctgctttccg ccgtgccgcg ggattccaac gcgcccttcg acatgcgggt catcgtcgag
    12121 atgatcgtgg acctgggcag ttttttcgag atcggggcga ccttcgggcc aagtcagatc
    12181 tgcgggctgg ccaggctgga cggccaaccc gtgggggtgc tggccaacga caatcggcac
    12241 tatgccggcg cgatgacggc ccaggcggcg cagaagtatc gtcgctttgt cgagatgtgc
    12301 gacacctttc atctgcccat cgtgaacttc gtggaccagc cgggcttcat gatcggacct
    12361 gaggccgagc gagcgggcac gatccgttac ggcatggcgg cggtggccgc cgccgttcag
    12421 gccaccgtgc cctgggcgtc gattcaggtg cacaagggtt tcggagtggc cacggccgcc
    12481 cattacggca agggtgctta cgttttggcc tggccgtcgg tggaaaccgg cgctttgccg
    12541 ctcgaagggg gcgttgccgt tgccttccac cgggagatcg ccgcttccga cgacccggac
    12601 gcgaagcggg cggagctcga gtccggaatg cgtgctgccc gttcgccctt tccccgggcg
    12661 gagtcgtttg ccgtgcacga gctcatcgat ccccgggaga cgcgtccgat gctgtgcgag
    12721 tgggtcgatt ggatccagcc gcgtctggac aacctcaccg gccccgttcg tttcggtatc
    12781 cggccctgat cacccggcgc ccagattgca ttagaatgcg cggctctctc gtttgcttag
    12841 ctgactacag ccctggagaa gaatatgact tattcgtttg aaggtaaggt tgccatcgtg
    12901 accggagccg gcggcggtct tggccgatgc cacgccctcg agctggcccg gcgaggggcg
    12961 aaggtcgtgg tcaacgacct cggtggcgcc atggacggaa ccggggggtc gtctgcggcg
    13021 gcagacgagg tcgtcgccga gatcaaggcc gggggcggcg aggcccttgc caatggcggg
    13081 tccgtatcgg accggcaggg tgcgaagagc atggtcgtcg atgccatgaa cgcatggggt
    13141 cgcgtggacg ttctgatcaa caacgcgggc attctgcgcg accgctcctt cggcaagatg
    13201 gagctggacg acttccagat tgtcgtcgac gtgcacctga tgggtgccgt caacgtcacc
    13261 cgcgaagtgt ggccgatcat gaaggaacag aactacgggc gaatcgtcat gacaacctcg
    13321 cccacgggtc tttacggaaa cttcgggcag accaactacg gcgcagccaa gcttggtcag
    13381 gtgggtttca tgaactcgct gaagatcgag ggcgccaaga acaacattta caccaacacc
    13441 atcgcgccgg tggcggccac gcggatgacg gaaggactca ttcccgaggg ctcgcaggtt
    13501 gcgctcggtc cggagctcgt gaccccggcc gttatcttcc tttgcagcga agaagcgccc
    13561 aacggcgtga tcgttcaggc tcagggcggg aactactcgg tggcctgcat cgtcgagaac
    13621 gaaggcgtca acctgggatt ggacgccact gcagaggatg tgggagcaaa cttcgagaag
    13681 atctcagatc tcaccggggc aaagccacgg ggcatgctgc agctcagcag catgtaggct
    13741 ggcggcagga tctttgcagc gcgccgacgg gcacatatgg caggggtaat actcacgctc
    13801 attctggcat tcgttgccgg cggcgcgcta tggattggca tcggcccgag gctgaagctc
    13861 gccgaggacg ccgagcgcaa cgggctgctc aacctgctcg catacgtcgc catcgctttg
    13921 ccggtggcgt tcgcggtggt gttctacctg ctggagctga tctgagctgg cgcgagggct
    13981 gaatcagttg tagccgcgga cgtagctgag attgtcctct gcgtcgtcct cgttgtagag
    14041 ctggtccggg aaggcaaagg gtgcctcggc cgcgaacagc tcctcgacgg gctctcgctc
    14101 gttttcgtcg tgcatcttgt tgaaccggca gggtcgtgtg aggggagtct agtgtcagat
    14161 gttgaaattg caatcccgtt ctgcgaggtt gccgtcggat ttgcgctgaa ttgcgacgaa
    14221 aagctcggtt tttcgacatg aagcttccac tcgatcccga cgagatcaaa gggttccttc
    14281 acccggagga aggcgcacgg ctctacgcgc tcgccagcgt tgcgtcgggc ctagggccct
    14341 gcctcgaggt gggcagctac tgcggcaagt ccacgcttta tctcggtacg gcctgccggg
    14401 accagggcca ggtgctctac gcggtcgatc accatcgggg atcggaggag caccagccgg
    14461 gtgaggcgta tcatgatccg gaactcttcg atgccgagat cggcagcatg gaatcgctga
    14521 tggcgtttcg cagaaccatg taccgcgcgg gtctggaagg atgcgtgatc ccggttgttg
    14581 cggcgtctgc gctggcgggc cgccactggg cgacgccgct tgcgctcgtg ttcattgacg
    14641 gtgggcacag cgtcgaagct gctcagaccg attaccggac ctgggctccc cacgtcgtgc
    14701 ccggcggttt cctggccatc cacgatctgt ttccggatcc tctcgaaggc ggccaggcgc
    14761 ctatagacgt ctaccgcctg gcgtcagcct ctggcctgtt cgagcctctg gaaacgaccc
    14821 gcacgctcgg agtgctccgg cgcgtcgggt agcgcattgt ccgtgaacag ggccgatgcc
    14881 ggggtcagcc cgtgcatggc gtcggcggcc agcagcacgg ccccggcggt ccaggtgggc
    14941 cgctcgtcgg gccacagggc gtcgtcctcg aacgcgtatc ccgtccacca agaaccgtcc
    15001 tcggcctgga acctcgccag gtcgtcgcag agctcctcgg ctttgtccgg ctcgccggaa
    15061 gcgacgcagg cgatggcaag ctcgcaggac tcggcaaccg tgacccaggc ctggtcggag
    15121 acgcagcggc agcccagcga aggggcgacg aaggtttccc atcgctcgtc cagccgttcg
    15181 cgtgcgtcgg cttgtgaaaa gagccccgcc agcaccggat agaaccagtc catggagtag
    15241 cgggacttgc tttcccacgt gcggtcgaag cgatcggggc gcaggatgat ggcatcgccg
    15301 agtcgcgccc ggcatgcggg ccaggcgccc gggtcctcat tgagctccgc ggccatgaag
    15361 atgccgcact cgaggctctt gtagatggag ctgcaaccgg tgaccagggc gtccttgttg
    15421 atgcccgtgc gggtatcctg ggcccagtag atctcgcccg tgggagcctg caggccggtg
    15481 acgaaatcaa gcgcgcgctt cactgtcggc cacatggagc gcaggaactt gccgtcctcg
    15541 gagatcagat aatgatgcca ggtgcctgtt gccacgtagg cgacgaaatt ggtctccgct
    15601 cgggtgccgt ccgccacgcg cgcaccgctg taggcagcca gccaggaacc atcggcccgc
    15661 tgcatccggg caagccagcg gtaagcggcc gcggcctgag cgtgatgccc gccgatgctc
    15721 agccccatgg cggcctccac atggtcccag ggatccgtga tccctcccgc aaaccagggg
    15781 atcgcgccgt cgttttgctg gacggaggcg atgtatcggg cggtgcggtg cagagattcc
    15841 ggccgcgtca tacggcaccc cggtgaaagt acatgaccac gcttttgccg atcagcgggt
    15901 tcagggcgcg ttccaggaca cgggtgagca ggggtttgcg catgagatcc cagaccagga
    15961 agcggtgata gaggcggacg ggccagaccg actcgccccg gcgccagaac aggcagcgca
    16021 gccaccagta cggggaatgc aggccgtggg cgaaatgccg gctctggaac gacagttgcg
    16081 tctttccggc caccggccgc gctccggccg gttctggggc gcacgcctcg atggaccttc
    16141 tcagctcccg ggacctgaag atgcgaacgt ggccgccctc cacctggtgg tagtcgtcgg
    16201 aaagtcgcca gcagagccac tccggaccga atcggggcac gctgaccgca agcgttccgc
    16261 cgggtttgag cactcgatgg atctcaccga gaacgttctc gtagtcgtgt atgtgctcga
    16321 gaacttccgc gcagatcacc ttgtcgaagg tggcgtcggc gaacggcagg ttgtgcccgg
    16381 tggcgaggat gaagtccacc cgccgagagg cgtcgtcgcc atcgcggaac tcgccgtagc
    16441 gttgccgcgc ggttgtcaga tcgccgagcg acagatccat gccgacgaca gtgacggcct
    16501 ccctgaggta ggcggtgatg gcatggcggc cctctccgca gccaagatcc aggacgttgt
    16561 ccccgggggc gagtttcagg cgcgcgaaat cgacggtctc cagactcatg cgagcacctg
    16621 ctcgtagaga gcggtgagcc gctctgcggc ttctgcccag gcaaagtgtt cgaccatgcg
    16681 ggcccggccg gcgcggctga gcgcttcgcg ctccttcggg tcgaccagaa gcccagcgat
    16741 ggcctctgcc agcgcgtccg ggtcgcggct cggcacgatc cggcccgcct gaccgacaac
    16801 ctctggaagc gcgccgccgt ccgtgctcac cagtggcgta ccgcaggcca tcgcctcgcc
    16861 ggccggtagt ccgaagccct catattccga cgggacgacg gccacggtgg attcggcata
    16921 gagcctccgg atgtctgccg tgctgatccc gtgaatgaaa tccatgcgat ggcgaacgcc
    16981 gagcgcgtct accaggcgat ccgttttgcc cccgggacgc ggggcgccta tgaagactag
    17041 gcgcagctcg ggaaagcgcc ggcacagctg ggcgaacgcg ggtagcaggt gctgggcgcc
    17101 cttgagtggc tgatcggcac tcgccgtggt gacgagttgc cagggtttgc gcgtcacggt
    17161 cggatccggg cggaacgcct cggtgtcgat gccgttcctg acgacggtca cccggccctg
    17221 ggggatctcg aaagccgcgc agatgtcgcg cttggaccgc tcgctgacgg tgacgatgtt
    17281 gggcagacgc tttgccacgc gggtctgcat ggcaagaaag ctgtgccagc gtcggatcag
    17341 cagccgctcg ccccgggttg cggcgttggc gatggcgata tcgcggtccc aggtgatggg
    17401 atggtgaatg gtgcagatca ccggcgttcg gcgcgcgacg ttcagcaggc cgtagcacag
    17461 gctctggttg tcgtgtacga cgtcgtagcg atcgtcgatt cgggagaagt atctcgtcag
    17521 gcgtcgcccg aaggtatacg gctccgggaa tccgccgctc agcatggaca accattcgaa
    17581 gaagtcggtt gccgaaagga gatgcttcgg gcgcagcgcg gtcacgtgat tgggcacgtc
    17641 gaacaggttc aggcctggca gcttgatcag cccgacgcgc ccgtccagtt ccgggtaggg
    17701 ctcgccggat atcacgtcca cctcgtgccc ggcatccacc aaggcgcggg agaggaactt
    17761 cagatacacg ccctgcccgc cactgaacgg gttgctgcga taccccagca gcgcgattcg
    17821 cagtggccgc cgaccagaag gcgcttcctg gccgaactcg taggtcgcga gctccgacat
    17881 gcctgccccg agcaaagcgg acaagtctaa tcagcggcgc gaaggagtgc caggcgttgg
    17941 acggcggatg agggtagggt cagccgttgc ccgcgtggat acggccagcg cgggaattct
    18001 cctgctttga tgggtcaggc tgggccgcag agccccccgc ctgggcccgc cgctcgatgt
    18061 tctcgtacag gctgacgacc caggcccggc cctcttcggt tgcgcgaagc agcttcagcg
    18121 ctttaggcag gtagggtgaa atctgctggc tgaaaaaagc ctgggaatcc gcgcgaagct
    18181 cggccacgct cgcgaacccg agccggtcag cctcgccgtt ttccttgaac tccacgaaca
    18241 ggctggacag ttttgccaga tagttcgggt cgcccatctg gccgatcaga tcggctgccc
    18301 gcacgagcgc gcccagggtt tccagctgct ggtagggtgg ccggtcggga accgggaagc
    18361 gcgtcatttc gatgtaggcg gccacgcgat cgacgtcgat cagcggatgg tctgcatagt
    18421 gttggcgaac gaagagatca ccgcgggtga cgtggtacgg catcatccag gcatctgtgg
    18481 cgtctgcggg gggcgatacc cgcctgccgc catgttccac caggcagccc tccggcccgt
    18541 cctcgggcag gaggtcccgc agatagccga cgtcgtgaaa gagcatggcg accaccgcat
    18601 gaagccagtc gtctggcgag atgggatctt ccagggcgag ctgccctttc aggatcgcca
    18661 gcccggcgtc cgccaccatg atggtgtgct cgacgttgtg gtaggggcag tcgcagcgtg
    18721 cgagcagtcc cagggccgtg gagacggcct cccgcagggt ttcgagatga tcttcgcggg
    18781 gctgcgcgag cgtgttcgcg aacgcctcga cataagcgtt cgtgcaatgt gcggcaaaag
    18841 cttcaacgac gattggattc gggttgaaca tcaagcagtc cggacaattc gaggcagttt
    18901 aaggcacggt ccccccagcc tgcgtgcctc agtcctcatt ctatgccata tcgggtaccg
    18961 ggagattgag attgaggcct cgtcgtttta tgaatattct gcacttaagg aaagaatttc
    19021 gtgagggaaa ttcatgtccc atctggagct caggcacctg aggaccctcg tagcgctgcg
    19081 ggacacgggc agcctcgtcg aggcggcgga acgggttttc ctgacccagt ccgcgctttc
    19141 ccaccagctc aaggacctgg agtcccgact cggcgtcacg ctgttcatcc gcaaaacgcg
    19201 acccgttcgc ttcaccaccg ccggccgccg actgctcaag ctcgccgacc aggtgctgcc
    19261 cctcgtccac ggcgccgagc acgacctgga gaggctggcc ggcggcgagt cgggaaggat
    19321 atacatggcc atcgagtgtc acagctgctt cgagtggctg ctgcccgcca tcaatgccta
    19381 tcgcgaggag tggccggatg tcgagctgga cattgccagc ggctttcact tcgctccgct
    19441 ccccgcgctc gctcgcggcg atctcgatct ggtgatcacc gcggacccca ttgaggagct
    19501 cgggctcgtc tacctgccgc tgttcagcta cgaagcgcag ctcgcggttg cccgtgatca
    19561 cgagctggcg gacaaaccct gggtggagcc cgccgatctg tcgcgcgagg tgctcatctg
    19621 ttatccggtg gaccgcagcc gcctcgacat cttcaccagc ttcctcgatc cggccaacgt
    19681 cgagccggct cgtgtgcgga acgcggagct gacgaccatg atgatccagc tggtggccag
    19741 cgggcgcggc gtggcatgcc tgcccaactg ggctctgcat gagtatcgcc agcgcgacta
    19801 cgttcaggtg aagagcctgg gagaggacgg cgtctggccc acgctctacg cggcggtccg
    19861 ggcggatcag gcggacgccg ccttcattcg aggcttcatc gacgttgcga agcagacgtg
    19921 cttccagaac ctggtcggta tcgtcaccgc ggacgtctga caggatcgac gacctggtcg
    19981 cggtggcggt gatcagggaa ccagcaccga ctgcacccag ccgtcttccg acagctcgaa
    20041 gtgccggcgg tcgtgaatgc cgttgctctg attgagatcc aggcgttcga ccgtcgtcgg
    20101 cgacaggtag agtccggccg ccgtctgcgg aggtgccagc ggctcgggaa gatcgatctg
    20161 ggccaggcgc tccagcagcg tgtcccggtc gggcagtggg gaactctgcg gaaggtggtt
    20221 ggtgtagaac cagtccagcc gcttgggcgg atccgggcgc tggtgccagc ggtcatgcac
    20281 gaggtgcgtc ggcacgcgct cggtgccgca ccgcaggcgg tattgcaggt tcaggaccgg
    20341 cagccagacc accaccgaaa tccgctcgtt taagctgatt tcccgccatt tggggctcgt
    20401 ctcgtttgcg aaaatcgcca ggcgttcgtt gacgtcgcgc agcaccagcg tccgtgcctg
    20461 gggatttcct gcggagtcga cggaagccac cgtgcagagg ttggcacagg ggtcttccag
    20521 gcgcctcgcc tccgcgcggt cgcgggtgag ttgggcaagc gggtccacta aaagcccggt
    20581 ggtgcttcgt tcgatggcgg gcgccggctt gccgacgtca ggcctcggcg tcgcctccgc
    20641 cctgagcggc cccggccggt gcgtcgctct cctcggtcag gcggcggcgg atctccgcga
    20701 cgtcgcgctg cacttcgctc aggcggaaaa tcaggtctgg catctgatcg gtgacgcgcc
    20761 acacgaggta ggcgatgccc agcagcacga taaagcaaag gaagaccatg cacatctccg
    20821 gttggttggg ggactcgcga ttaggtatac cgactccatc gccgggagtc cagcggatga
    20881 acgtgaacgc ggctgccctt tgggtttcca gcgcaaccgt ttcaccgctc gtatgcagcg
    20941 tgggctgatt gcctccgcgt cgcgggtcat gaaatctgat gcgctatcga gtcgttcccg
    21001 tccagctctg caacggttgc ttcctgttca ggatcgacaa accaggctag gacggaattt
    21061 cctttggcgc cgtcaggatt tccagatctg cgcgctgctc ccctacgcta tgccatgggg
    21121 ccgcgacgtc gaagcgcgcc acgccgaagg tcggcagatt ctccgtcacc aggttgccgg
    21181 ccagcaggtt gaccaggtcg gtgagaccag ggttgtgggc caccagcgcc acgctggcaa
    21241 ccgcttccgg agtgcctctc agcacgtcca aggcgccgcg cgcgctggcg tgatacaggg
    21301 cgtgctcctc tacgaccagc gggcgtgcgg ccgagaagcc ccgctcgacg aaagctgcgg
    21361 tggctcgggc ccttgcggcg tcgctggtcc agatccactc tgcgggcgcg tcctggcttg
    21421 cgagccaggc ggccatgcgg ggcccgtcgg cccggccgcg gtcattgagt ggtcgatcga
    21481 agtcggacag acctggctgc gcccatgatg acttggcgtg acggatgatc cagatcttca
    21541 cggggccggc gttcagcggg tcatcaaggg gccgccgaag ccggcaggaa gcggtttcgg
    21601 cgtcaccgtc acgatcgcgc cgtccgctcc ctcctcgatg ggaaacgtgc gaatgccggt
    21661 gaacgccggt ggcccctgat gggcgccatc tctgatgtcg aaacgggcgc tgtggacggg
    21721 gcaggtgatc atgtagcccg aaagcctgcc ccggcagagc ggcttgttgg cgtggctgca
    21781 gagatcctcg atggcgtaga gctcgcctcg gacgcggcag acgaggacct gcgtaccctc
    21841 cgcgtcgaag gagcgcttgt cgccttcttc caggtcgctc agaggaccca ggttgatttg
    21901 ctttgcttcg ctcatcgcct cgctcacagc tgcccgttga tgtaacggtc gatattctcg
    21961 tggaaaaagc gtatgcggcg ttcctgggtg ggcatgtact cgccgcggaa cccgcgagac
    22021 gtcaggcctt tctgctgagt cgaccagatg gcaacgtcct gatcgattcc gggcccgcag
    22081 gacacctcgc cgaccttgcc gcgctcatgg gcggcggggg tctcgacgga cacccagtcg
    22141 cgcatggagt tggagtagta ctccttggcg ccctccggaa acaaggtcag gtaccacatg
    22201 tcgaacaggc atttctccgg atcggtgggg tgtgggttgc cgcgcaggaa gatgcacccg
    22261 tcgggcttca tgctgaacga gaggttcgga aacagcgtgt agtggtagtt gtccgtgagc
    22321 tgctcgtccg aatatcgcga gaagtcgtag cccttcttct gggccagctt gcgcttctgc
    22381 tgctgcagcg cggtccgcaa accggcgagg tcgtctcgga agggttccgg gtcgatctcc
    22441 cagaacgcca ggtcctgacc gaggcgctcg aacacggcct ccggggagcc ctgatagttg
    22501 ctgttgggcc cgccaccggg catgaacatg cggcagtgac cgctggggta gagatcgaac
    22561 tggcagctcg agtagtgctc gtccaggctg atacacgatt gcgggtgcac gtatggcagg
    22621 tgatagctct cgttgaaatt gtcctggacg cacttccagt tgaagttgcc ctcgagggtc
    22681 acccagtggg ttcggcgcat gtcttccata cgatagttgt cgagctgtcg ggcaaccggg
    22741 tcgagaaaat cggcgagagg tttgcagtcg gggtccatgt tgtaccagat gaaccccgcc
    22801 cacgtagcgc acgggatctc gatcagattg cgcttgccgc aggggttgcc ctgggggaag
    22861 tcctcctcgc agtagaccca tgtgaggaca ccctgagcat cgaagcgcca gccatggtag
    22921 gcgcactgga actcgccgcc tgacagggat ccctgctccg cctgaaccag ctgattgccg
    22981 cgatgctggc agacgttgta gaacgcgcgg atgctgccgt cgtcgccgcg cacgaacagg
    23041 atggattcgc ggccgatgtc cgtggtgaag aagtcgcccg gctccgggat ctgatggacc
    23101 aggccgccga tcagccagac gcgcgtccac acgtgctccc actcgagctc ggcgaactcc
    23161 ctggaccggt aacggtcgcc gttgatcggg ttgctcccaa gctcgggctc cgtggccttg
    23221 gtccgatcaa ttcctcgcat ctgccttatg ctgctcatgg catgctcctg tctggttgac
    23281 cgcacggcgg gtgcgcacgg acggcttatt ctgcccgctt gggggtggca ttcaagcccg
    23341 gtgcgcgggc cggcgaacgc tcagctccag atctcgaaaa actcctcgaa cgccttgagc
    23401 tggttgccca cggcggacga gggcacgatg atgggcgtgc ggacgccggc gtcgaaccag
    23461 gcctcgatgc cgtcgcggac cgcgttcgcg ggaccgaaca aagtggtctc ggcgagccac
    23521 gcatcggaca ggcaatcggg gatcctgtcg ggctcccctg cctcgagcgc cttctccacg
    23581 gcacccatct cctccacgaa gccggcttct ttccagtagt tgcggtagtt cgggagcatg
    23641 gcgtaacccg tcagggtccg gcggttgacg gcttttgccg cctccacgtc gtcgctgatg
    23701 caggtgggga tcatgttgcc gatgaagaaa ccggggtcgg cgcgccgctc gtcaccgagc
    23761 acgttcagcg agtgctgcat atggccgcgc gcggcgttgg cgaacaccat gccctcggcg
    23821 acctccgcgg caagcccgat catcctgtcg cgcagcgtgg ccagcaccac gggcggcagc
    23881 tcacctaccc gcggcgcggc gcgcaggtcg ctcacgaact ggcgcatgtc cgacaggggc
    23941 ttgccctgcc ggacgcccag acgctccatg gccggtgcgt ggctgacccc cagcccgaag
    24001 cggaaccggc cattggagat ctcgtgaatg aatgccgcgg tggaagcgaa gtcgttcgcg
    24061 tggcgggtgt agatcggcgt gatgctggtc ccgaactcga tctgtcgggt gttgtaggca
    24121 atggcctcgc acagcgccag gccgtctccc atgctcgggc agtagatgcc cgcaaaaccg
    24181 cggcgctcga tctctgcggc gagctcgacg atggtgttac ggcggcccgg gaccgcggcg
    24241 aggcttactg cgggtcgtcg atcgctcatg ctgagttctc cctgggaagt atgcggaaag
    24301 cgacacagag tggcggaacc tcgtgcgctt cgcaaccgcg tttgccgcca tgttttcggc
    24361 tgcggcggcc gcgcgtctca gaaccagcgg tgtcgcgagg agcgcctctc gggctgggcg
    24421 agatgctgtg cgaggtgatt cggccggcgg aacaggtgta tcagagccac cccggccaga
    24481 aacccgccga tgtgagccca gaaggcgacg cccccctggc tcgagcccat tgccggcaaa
    24541 ccctgaagca gctgcaggag aaaccagtag cccagcatga aaaccgcggg aacggccacg
    24601 gtggtcacgt agaagccgag aaagatcagc gtgtgcacgt gggcgcgtgg gaacatgagg
    24661 gcgtaggcac ccatgacgcc gccgatggcg cccgatgcgc cgaccatcgg caccaggctg
    24721 gacgtgttcg tggcgatctg ggcgaaggcg gcggccaggc cgcagacgac gtagaagacg
    24781 acgaaccgga acggtcccat gacgtcctcc acgttgtctc cgaaaaccca cagaaaccac
    24841 atgttcccga tgatgtgaaa ccagccgcca tgcatgaaca tggacgtgaa gagggaggca
    24901 ggattggcgg tcccgtcaag ctggcagacg aggttgctgc ccagcggaat gctcgtgccg
    24961 ggaacggcgt tgcccagcag gtcgcccggt atcagcccgt acagacacag ggactcggcc
    25021 agcgcgtagt ggttgccaaa tccctggacg gcgacccaga cgaggacgtt tagaatgatg
    25081 atggcaacgg tggcgtacgg cgggtgaacc cggggatttt cgtcgcgcag tggaaacatg
    25141 atcggctcgt atggacctca ggcacggtgt tggctcggcg cgtcggcaga tgggcgcatg
    25201 aaaatctagg gtgccgggat gtcggcgccg ccgcaagacc gggcatggtc ttgaaggctc
    25261 agaacgtcag gctgatcttg tccgccgtgg gcaggtcgcc cggctcgagc gcctcgcgct
    25321 tggcggcctt gaacgcttcg atatcgccaa cgaccatctg ccagaccggg tgcatgtccc
    25381 gcccgtccac cggcatggga aaggggctga agccaagggg cacgctggcg aacggctcgt
    25441 cttggcttcg gtagagagtc tgctcggaaa aatccagata cctgggcaga ccatcgcgcg
    25501 agaccatgtc gaagaaagcg acggcacccc gaccggccag gtccagacgc cggatcatct
    25561 ctgcgggaaa caggccgccc gtccagcggg cgttgatctc ggtgaccagg agctcatcgg
    25621 cactgacgaa gtagtcgatc ccgcagttga atccttccgg ggagcggtag ccgaggtctc
    25681 tcacgtactc cccgacgcga aggagcgcgc gctcgtgggc ggggtccacg gtcacgccgc
    25741 cgccgatcag atttcccacc cagagcccgt cggcgaacag gatgcgccgg acgttggcga
    25801 tctctatgct gtcgtcccgc acgatcaggt ccacggtcat ctcggtgaac tcgtccgcgc
    25861 tcagcttccg ttgcagcacg atctccatgt cgttcgggta ctcggcgacg tagtccatgc
    25921 actccggcac gccagccacg ctggtgacgt tgcgcgcgcc ggccagaccc ttcagcttca
    25981 gcatgatctg gttgtggtgg gtgtcgaaaa agccggatac ctcggggccg tcgaggtcgc
    26041 gcttccggca gaccagggtt tcgggcaccg gtatgcccca ttgcgcagcg tccgccgcca
    26101 gctgcatctt cgagttcacg cgttggctga cggaaagcgc ggcgtcgctg cggtgcacca
    26161 cggcgttgga tccgctcacc aggtagaggg tggtcagatc cgcgtcgacc cggtccgccg
    26221 ccaccgcctc gtagaacccg tcccgtggat agtagtgaca gttggactcg ggcacgggcg
    26281 gcacgcccat ctcggtgaac gcgcgacgat acaagtccag cgtctcgcgc cacggcggtc
    26341 gctgaaactc gtcgaacaag gcgacgtgat ccacgggctt gccgacgtag agcagagcca
    26401 cctgaagcat ggccgtgagc tctatgttcg atacgtgatc ggccagctgc ttctcggtca
    26461 gcgtgttcag gtcgaagtgc aaagagcagt cgtcgccggc catggcgtag agcgtggatg
    26521 ccgcctggct ggggaaatac gaacccgcct ttggtgtcat gatctgactc tccgtcgggg
    26581 ttgcgatgtg ttggccgaag ctgcggacga tagcgggcgc gcagcggtga ttgcgaagcc
    26641 gtgcatacta atccctactc tccgcggctc aggatgtccc ccacccaggt cagcagggtc
    26701 tggttctgct cgggcgtgcc cacggtgatg cgcagggtgt cccgcaagct gggggtgtcg
    26761 aagtaccgca ccagaactcc ccgggtcttg agcgcctcgt agagctgctt tgcgctgcac
    26821 ggagacgccg cgggcacctc ggtcagcaga aaattcgtct gcgaagcgcc aacgcggaaa
    26881 ccgtgttcga gcaggccctc gcgaagcgtg cggcggccgg cgcgtacgtt tgcccaggtc
    26941 tcttcggcat aggccggatc ggacagcgcg gcctcgccga cccgctggct cacgtagtca
    27001 acgttgtagc tgtcgcgcgt tttgccgatg acggggtcga tcagcgaggc gcagccgacg
    27061 aggtagccga gacgcattcc ggccaggctg taccccttgc tgaacgtgcg caggatgagc
    27121 agattgtcga acgcctcggt gaggcgcacc gcatcgtagc gaagggccgg gtcgacgaaa
    27181 tcgacataag cctcgtcgat cagcagaacg ccgttgaaat cattggccag ctggctgatc
    27241 cgcgacgtgt cgagcaggga gccggatggg gcatggggat tgaccaggca ggcgagtccc
    27301 gcccccgcgt cgttgagctg gtgggcgaag tccctcggca ggtcccagtt ctcgtccagc
    27361 gcgatgctca ggacccgggt gtcctgaatg gccgccagaa cgggatagag cgagtagctg
    27421 ggatcggcca taccgaaggt gccgcccggc tccaggaacg tggtgaaggc cagcctcaag
    27481 ccttcgtcgc cgccgttggt gaccacgatg ttctcgcgct tcaggttgtg cagctcggcg
    27541 atctgatcgc gcaggcgatc ggccgtgggc tgggggtagg cgcgcagggc ctcgccgctg
    27601 agatcggcga gcgcccggtc cacggccggg ctcggcgggt aggggttctc gttggtgttg
    27661 agcttgatga cgtcggctcg ttccggctgc tcgccccagg tgtagccgtg catggcgcgt
    27721 atgttttgac gttcgtgggt catgtctggt cctcggccgg cgtgggatct gcggcccgtt
    27781 cggcaggtcc gagcagcacc gccagccact ggctcgacgg gttcgcttcc atggcgatct
    27841 tggcggtcat cgtcagcggc accgacagca gcatacccac gggtccgagc atccagcccc
    27901 agaacaccag ggagacgaac accaccagcg tggaaagtcc cagcccccgg cccatgtagc
    27961 ggggctcgac cacgttcccc atcagcaggt tgatgacgaa gaacccggcc ccggccaccg
    28021 cggccgtcag cgggccgagc tggatcagcg cgagcagcac cgcggggatc gccgcgatca
    28081 gcgagccgat ggtgggcacg tagttcagga gaaaggcgag caggccccaa agcgcgggga
    28141 agtccacgcc gagcacggcc agcatcagcc cgacgagaac gccggtgatg atgctgatgg
    28201 tggtcttgat gccgatgtag cgattgacgt tttccgcgaa gcgaacgaag tggggcaggt
    28261 cgctgtccgg atcgtctagc acctcctgaa gcttgcgggg aaagctcgag gcctcggcca
    28321 ggatgaagat gacggtgagc aggatcagga aaccgttgct gagcgcgcct cccagcccgc
    28381 gcagggtatt gccggccatg accagggcgg tgcccggatc gaagtaggac agcagggcct
    28441 cctcggagat gttcaccccc gccatctcca cgtaggtcac cgcgttctcg acgagctccg
    28501 tgagccggtc ctggtagaag ggcaaccggt tcgtgaaagc gcccgccgac tgggccacca
    28561 ccgcgccgac cccggtgatc atcacgatga tgccgagcat cacgagagcg atggcgattc
    28621 ccgccgggac ccgacggcgc tcgagccaga aaacgggggt agccgcgatg gtggcgatga
    28681 aggccgccag caggaaaggc accatcagcg cctctgccgc cttgatgcct gcagcgatga
    28741 cgatgagggt tgccacgacg atgagcgtct tgctcgttga acccaatgca gccgtccgtg
    28801 agggcctgtg ggtcattcta ggagacgtct gtgtgtgagg ctaggcagct cctccctgac
    28861 ccgccgcacc ctttcggggt ctatgtctgc caccgccacg ccatcgccgt cccggcattc
    28921 ggcaacgatg tcgccccacg gatcgacgat cagggagtga ccgtaggaga agcgtttgcc
    28981 ccagttgtgg ccgtgctgtg ccggggcgat gacgtagctc tggcactcga tggcccgcgc
    29041 ccgcagcagc acgtgccagt gagcggcgcc ggtggtcttg gtgaacgccg atggaaccgt
    29101 cagcgcaacg gcgcccgcgt ccaccaggga ctgataaaga taggggaacc ggaggtcgta
    29161 gcagatgctc aagcccagct tgccgaaggg catgtccgtg gtgaccaggt catggccggc
    29221 cagcgtgcgg gccgactcgc gcagctgggt gccgtctgcc agcgccacgt cgaacaggtg
    29281 cacttttcgg tatcgagcca gcaactcgcc gccgggagaa aggtgaacac aggtgttgta
    29341 gctcttgtcg ggatcgcctg catcctcgtg aaagccgccg agtatcaggt ggcagcccag
    29401 gcgcgcggcc attgaccggc actgttccag gatcggaccg cccggcgcgc tcggggcgtc
    29461 ggggagccgc tccaggatgc cgcgctttgc cttttccggt ccgagaaatg cgaaggcctc
    29521 gggcagcagc acgacctcgg cgcccgcatc gcaggcctgg gggacgagac gctcgacggc
    29581 aatcatgttg gccgtgacgt ccggcgtcgc gcatagctgg acgacgccta ccctggttag
    29641 acggtggcct tggttcacgg caccggggtc ccctgcggct tgggcggtac cgttgcccgc
    29701 tcagaagctc ggctgggtgc agaccacggc ctcaccaaga tattcctcga tgctgggcag
    29761 gttgaacgcg tcgtcttccg agacgaagct gatgctcaca ccctctgccc cggcgcgacc
    29821 tgtacggccg atgcgatgca cgtagtcctc gggatcttcc gggaggtcga agttgatgac
    29881 gtggcttatc gcctcgacgt ggatgcccct gccggccacg tccgttgcca cgacgtaggg
    29941 gatgtcgccg tccttgaacc gcgacagcgt cgaaaggcgt tttctctggg gcacgtcgcc
    30001 tgccagggcc tcgcagcgga tattgcgctt gttgaggaac gcgtggagct ggtgggtctg
    30061 atcacggcgg ttcgtgaata tcagagcccg ctccggcgca tgcctggtga ggaactccgc
    30121 gagcagcgaa gcctttttgt cggcggcgac cagccagaac ttctgggtga ccgtatccgt
    30181 cgccacggat tccggggtga tggcgacgtg ctctggatcg atggtccagg aattggccag
    30241 ccgcatcacg tcgtcattga aggttgcact gaagaacagc gtctgccggt tccgtttgtg
    30301 gggcgtctga tagacgatgc ggcggacgtc gggaacgaag cccatgtcga gcattcggtc
    30361 ggcctcgtcg atgaccagga cctccacgtc ccgcaggctc aggtgacggc ggctgatgaa
    30421 gtcgagcagc cggccggggg tggcgatcag cacgtcgacg ggacgccgct tgatgcggtc
    30481 cagctgcttc tgaaagtcca tgccgccgac cacgcactcc acaccgaggt cgatgtactt
    30541 ggtcaggtcc ttggcatcgc tttcgatctg cagagcgagt tcccgggtcg gtgccagcac
    30601 cagcgaacga ggcgaaccga tcttgggcgc cgcggcggga gggtactccc acaacctggc
    30661 aatgatcgtg accaggaacg ccgccgtctt tccggttccg gtctgggcct ggccggtgac
    30721 gtcgtagtcg gcgaggctcc acggcagcac cgcgctctga atgggcgtgc agtactcgaa
    30781 ctcgaggtct gcgatggcgt gcatcagagg atctggcagg gcgaaatcgt gaaagcgatc
    30841 cttgccctcg gttggggcta ctgggaaatc ctcgatgcgc cacttactca tgagcttttt
    30901 gtgctcctcc tgtgtcggtc cgttgatgga tctccgcgcc gacggcggcg agcttgacgc
    30961 aaacgacgag caactccatc gcgacgttga gatccgcgag gcttgcaaac gtgggcgcga
    31021 tccgcaggtt gcggtcctcg ggatcccggc cgtagggaaa cgttgcgccg ggcttggtga
    31081 tcgtcaatcc ggcctgggct gccagctcgc ccacctcttt cgccaggccg ggtcgtgtgt
    31141 ccagagacac gaaatatccg cccgccggcg tggtccagct tgcgatttcc aagcccgcca
    31201 gaccttcgcg caacttgctt tcgaccaggt cgaatttcgg cttcaccagc gccgcgtgcc
    31261 cggccatgtg ggcctccacc cggccgccga ggaaccgcgc gtggcgcagc tgattgacct
    31321 tgtccgggcc tatgctcgcc gtgccgaggc gcttctccac cgtctttcgc accttggcgt
    31381 cggcagccag gaaggccaga cccgcgctag ggtaggtgat cttggatgtg gaggcgaaca
    31441 tgatcacgtg atcggccgtg ccatgcttgc gcgcgagatc gagcagggag gccagaggcg
    31501 cccgcggaaa ttcgaagtcg tgcacggcgt aagcattgtc ccacaggatc agaaaatcgt
    31561 ccgccgccgc atccgccggc agccgggcca gcgcgtccac gcactccggt gagtagatgc
    31621 accccgaggg attggaatac ttaggcacgc accagatgcc cttcacggac gcatcctcgg
    31681 ttgcccttcg aacggcgtcc atgtccggac ccgtatccgt catgggcacg ttgaccatgt
    31741 cgataccggc cgcttcgcag atggtgaagt ggcggtcgta gccgggaacc ggggtcagca
    31801 ccttgggatt tgcgtcgcgc cgccatcgcc tcccgtcgcc ccacaggccc tgattcagag
    31861 ccagatcgac gcagatgtgc atgagctgca ggctcgagtt cccccagcag atgatgtccc
    31921 cggggtcggc ctccatgatc tctgcgccga gttccctggc ttcacccagg ccagtgagag
    31981 cgccgtagtt gcgcgagtcc gttccgtccg aagcgatgta gttgcccgcg atggcatcgt
    32041 ccaggcccgc gctcaggtcg agctgatccg cggccggctt cccgcgcgtg agatccagcg
    32101 acagacggtt cccggcctgg agttccagct cccgggacag ctcggccttc agcgctgcga
    32161 gctcggggtc ggaaagcgcg gcgatggctc gtgccgtcgt catgatgtca ggcccgtggt
    32221 ctcgtcgatc cccatcatga ggttcaggtt ctgaaccgcg gccccggcgg cccctttgcc
    32281 gagattgtcg taccgcgcca tcagcagcag ctgctccgag tgcccgaaca gcatgatctc
    32341 gagccggttg gtgccgttgc aggcggtggc gttgagaaaa ccgtccgtca gggcggtctc
    32401 cgcaccgggc tcaagcacgt cgacgaaagg ttccaaggcg taggcttcct cgaggatgtg
    32461 ataaatgccg tcatagtctg cgcccttgcg gagttggctg caaaacaacg ggaccccaac
    32521 cagcatgccc tggtagaact ggcccaccga cggcgtaaac agcggggcga tcttcgttcc
    32581 cgagtaagcc tgcatctcgg ggacgtgctt gtgcttgagc gacaaagcat agggctcggt
    32641 attgcggcgc tcggcctctt gggaaccgaa ggccctgcga gcctcgatga gcgtgcgccc
    32701 tcccccggaa tagccggaaa gcgcctgcac ggccaggggc tgctcagggt ccagcaggcc
    32761 tgcgccgatg agggggcgga tcatgaggat gaagccctgg ggatagcaac cgggattggc
    32821 gaccctttct gccgcggcaa gggcctggcg ttggttggat cctagctcag gcagaccgta
    32881 gcaccatccc tcctgaatcc ggtgggccgt gctggcgtcg atgatgcggc agcggccgtc
    32941 cgccagaact gcggcctcgc gcgccgcgtc gtcgggcagg cacaggacgg caacgtcggc
    33001 agagtcgaac agccgctgtc gggccgcggg tgatttgcgc tcggcgggat cgatctccag
    33061 caactcgacg tcgtcgcgcg ccgccaggcg ctggacgatc tcgaggcccg tggtgccggc
    33121 ctgtccgtcg atgaataccc tgatcatggt gccgaggtcc ttccccgcgg ggttgtttct
    33181 taggggcgcg cgattttagg caatatcggc tcgatgtcac agcggcgcga cggaaaagat
    33241 ggcccctcgg ctgttccggc acccgtttgc ccgtctgtgc cccccgcgga tccccgggtc
    33301 aatcgcggcc ggggagccgg ggaacgaact tgggcgtgtt ggatacgtac tcctcgtaat
    33361 cgggatgccg gcgagacatc ccttgctcga gaatgtcggc ggtgagcacg agaaatatca
    33421 tggtcatgac cacagggccg agcagcgccc accacgcggc gcccgacagc gcgacgatgg
    33481 acagacccca ccactgcaac gcgtcgccga agtagttggg gtggcgggac aggccccaca
    33541 ggcccgtgtt gagcagctgg ccccggttgg caggatctgc cttgaatcgg ctgagctgaa
    33601 cgtcggcgac gagctcgaag acgaaaccgg cggcccagat caggacgccg gcgaggttca
    33661 gcagcgtcag gtcccggggt gcgtagaagc tggcgaggat gggcgtggcg acgagcaggg
    33721 cgagaaatcc ctgcaggagg aagattcggt ggtaggttct cagccaccac ttcggtccgc
    33781 ctgacgctcg ccatcgggca tagcggacgt cctcgccggc ggcgagattg cgaccgatca
    33841 ggtgataggc cagccgaagg ccccacaggg tgacgagcag catgaccagg taaggcttgg
    33901 cctgcacacc gccaccgtag acgggcagca gcacccaggc tatggccacg aacagcggcc
    33961 cccagaacat gtcgatgatg cttgcgtcct tggtcggcac gctgacggcc cacagtgtcg
    34021 tgaccatcac ccatacggtc agtgcggcga ccccgtacag ctcgaacagt cccatgtcta
    34081 tctccactct atctccgctc gatcctcgcg gccgttactg aattcgcggg tgcgccttag
    34141 ggccacccga gttctcgctc tttcccgacg catcagcggt agatggtgtt cagccacgtg
    34201 ccgatgtcgg cgatctcctc cgggcacacc tggtggccca tgccgtactc gtgccactcg
    34261 acctcatagt tcatcgccag cagcgcctcc cgcgaggtga tcgcacgggt gatggggatc
    34321 atcggatctc caatgccgtg tgccatgaag atgggggcgt ccacgttggc gaacccgatc
    34381 cgatccacca ggtgctcgtg atcgggcacg taggtggaga gtgccatgat cccggccagc
    34441 ctgtggggga acgacaggcc cagctccagc gcgatgacgc ccccctggga aaaccccgcg
    34501 agcgtgatgc catcgtatgg cacgccgcgg tcgtgctcgc gctctaccag ccccgcgatc
    34561 gccttggccg aggattgaat gtccgaatcg ccggacagcg ggttcccggg atcgatgtcg
    34621 taccaggcgc gcatttccat gccggcattg atggtgaccg ggcgaacggg cgcgttgggg
    34681 aacacgaagc gcagcggccg gttcagcggc agcaggggca ccactggttc gaagtcgtgc
    34741 gcgtcggcgc ccagcccgtg cagccatatc accgaaccgg cggttccgcc cgggtcgatc
    34801 tcgacgcagt ccagcagatc agtcaccgta tttcccgacg ggcgagctcc gcggcagcta
    34861 aacaagatct cggggggaaa gcaatctccg tttctctcgc cagggtcggt caacgcggcg
    34921 gccctacgtt cgcggccctg caggtgagat actacggcca tggaggacgg actcgacaga
    34981 caggcatcac gggagctgac gcggctgctg cccgctctgg gctttctgcg cccgtatgtt
    35041 cgccaggtgg tattcgcgag cattgccctc gtcgtgacgg cgggcgtgac gctttccata
    35101 ggccagggga tgcgcctggt catcgacgaa ggcctgggca gcggatcggc cgccgtgctc
    35161 gtgcagagca tatcgatttt cgccgttctc atggtggttc tcacggccgg gacgtttctg
    35221 cgtttctact tcgtctcctg ggtgggagag cgcgtcagcg cggacatccg ccaggcggtc
    35281 ttcgctcatc tcatccgcct tcatccaggt ttcttcgaga cgaatctgcc caccgagatc
    35341 cagtcgcgga tcaccaccga cacgaccgtg ctgcagaccg tcatcggatc gtccgtctcc
    35401 atcgccctgc gcaatgtcct gatgttcgtc ggcgggctgg tgctcctgtt cgtgaccaat
    35461 ccgaagctct cgctgatcgt ggtggccagc gtgcccttcg tggtggcccc ggtgatcctg
    35521 ttcggccggc gcgtgcgggc gctgtcgcgc gacagccagg acacccttgc cgaggtggga
    35581 agctttgccg gcgagtcgct gcgcaacatc aagatcgttc agtccttcaa tcacgaaggg
    35641 ctggacatcg aagcgttccg gcagcgggtg gagcgggctt ttcaggttgc cgtccgccgt
    35701 atcctgcagc gctcgtggct ggtcgccctg gtcatgatgc tggtgctcgg ggccatcgcg
    35761 accatgctct gggtgggggg gcgggacgtg ctcgccggcc agacctcgcc cggagagctt
    35821 gccgccttca tcttctatgc cttcatcgtc gcaggctcgg tcggtgccat cagcgaggtc
    35881 tacagcgacc tgcagcgagc tgccggtgcc atggagcgcc tcgtggagct gctgaactcg
    35941 ccatcgctcc tgaccgatcc cgagtcgccc gtcctgccgg ctgcccggga gccgtcggtg
    36001 gcggtttctc cgggtggccg cctgacggtc cggaacttgg ggtttgcata tccgagccgg
    36061 ccggatgcgc ccgtgctgcg cggtctgagc tttgctgtgg ccccgggaga gatggtcgcc
    36121 ctggtggggc cgtcgggcgt gggaaagagc acgctgttcg atctgctgca gcgtttttac
    36181 gatccggacg ccgggcagat tctgctcgac ggcgtcgacc tgagggagtt acggcttgcc
    36241 gacgttcgtg aacgggtggg tttggtgccc caggatcctg tgctctttgc tggtaccgta
    36301 gagagcaacc tgatctacgg cgcgcccgac gcgggcgatg acgaaaaatc ccgcgcgctc
    36361 gagctcgccc atgccagcgc cttcatcgag gctctgcccg agggcctggc cacggtggtg
    36421 ggcgagggtg gcgtcgggct ctccggaggg cagaagcagc ggctggccat cgcccgcgcc
    36481 cttctgaaac gaccggagat cctgctgctc gatgaggcga ccagcgcgct tgatgccgag
    36541 agcgagcagc acatccgggc gagcatcacc gaactcaagg gccaatgcag catcgtcgtc
    36601 atcgcccacc gtctgtccac ggtccgcgag gcggatcgga tactcgtgct gagcgaggga
    36661 cggctccagg ccagcgggcg ccacgaggag cttctcaaaa gcagtgagct gtatgcccgt
    36721 ttctcgcgta ttcagttcgc cggcgaggac gaagcggacg gctcgggtgc tagaaccacc
    36781 gcgggatgat gcccacgcaa aagatcgcta aactcctcgg ctgatagtct gatttcttac
    36841 cggggggaag gtgagcatgg ggccgttaca cgggtttcgt attgttgagc tggctgggat
    36901 cggtcccgga cctttctgcg ggatgatgct gtccgacatg ggcgccgagg tgatccgggt
    36961 ggaccggatc agcggcgggg ccaaccgccc gaaggacgtg cttgcccgca atcgacgctc
    37021 catcgccgtg gacctgaaga gcccggcggg cgcggaaacc gtgctgcgca tcgtcgagaa
    37081 atcggatgcg ctgttcgagg gtttccgccc gggcgtgacc gagcggctcg gccttggtcc
    37141 cgaggattgc tgggcgcgca acgagaagct cgtttacggg cgcatgacgg gctggggcca
    37201 ggaagggccg ctggctcagg cggccgggca cgacatcaac tacatcggtc tgtcaggggc
    37261 tcttcacgcg atcggtcacg cgggggaccg cccggtgccg ccgctgaacc tcgtcggcga
    37321 tttcggaggc ggcgggatgc tgctcgccta cggcctggtc tgcggcctgc tcgaagccca
    37381 gaaatccggc aaggggcagg tggtagatgc ggccatggtg gacggggcgg cggccctgat
    37441 ggccatgttc ttctccatgg gcgctgccgg ggtgttcaag gaccagcgcg gcaccaacct
    37501 gctcgacagc ggctcgcact tctacgacac ctacgagacg aaggacggga agtacgtctg
    37561 catcggctcc atcgagcccc agttctacgc cctgctggtc gagaaagcag gcctggacgc
    37621 ggaacgcttt gccgcccaga tggacgcctc cagatggccg gactacaagc gtgagctgac
    37681 cgaggtgttc aaaagcaaga cgcgcgacga gtggtgcgaa atcatggaag gcagcgatgt
    37741 ctgcttcgct cccgtgctgt ccattttcga agcccccgac catcctcaca gccaggcccg
    37801 caacgccttc atcgagctgg acggcgtcgt ccagcccgcg ccggcccctc gcttctcccg
    37861 aaccgaggtg gcggccagcc acggtgcccg ggtacccggc gaggactcgg tatcggtgct
    37921 ggaggactgc ggatttacag gagacgagat cgccgggctc aagaatgacg gcgtgatcgg
    37981 cggcaactga ccggaggatc ggcagatggc ggtagagttg ctggacacgg agcgttttca
    38041 cggattccgt gtgcgcaggc agatcgctgg gaagacctat caggagtatt tctctctcaa
    38101 ggacgacggc aagcgcatca aaggtccgca gcgggccacg gtcaaagccc tggccgaagc
    38161 ccgcgatgcc gaactcgcga atctgcagaa agcggcgcgg gaaagagcgg acaggacggt
    38221 gcgcgtagat gccagaggcc aggttcgcgg aatcctgtgt cggctcaaga ccgagaagag
    38281 cggtaccctg acaccggtat ttcagatcgg cgtcatgtcg cgcctggaag acaagatagt
    38341 caacacgacc gtttcgatca gccgctacgg cgagacggag gcctggcgcc gcgccatcga
    38401 cttctacgcg ctgcacaaag ggatcagcaa acgatcccgc gcctatcgtg acctgctggc
    38461 agcgcaaccg agcggcgcga agatcaagag cctgctcaag gcgtccgggc gcgccgcacc
    38521 gcggcgatcc gcccgaagat gagctacgcg tcagagtacg cgcgatcgct ctctgatcca
    38581 gaagggttct ggcgggaaaa agcggcagag cttccctggc tcgagtttcc gcagaccata
    38641 ctggaccgcg cgccgtcggg cgcctggcgc tggtttcacg gcggcaagac gaacacctgc
    38701 tggctggccc tggacaggca cgtggcggac ggccgcgggg ctcaggtcgc gctcatctac
    38761 gattcgcccg cgaccggcag tgtccagcgg ttcactttcg cagagctcac ggagcgggtg
    38821 gccgcctgcg cgggtgggct tgcggccctc ggcgtgtcca ggggcgatcg tgtcatcgtc
    38881 tacatgccca tgattcccga ggcggcgatt gcgatgctgg cctgtgcgcg gatcggtgcc
    38941 attcactctg tggttttcgg cgggtttgcc gcgcctgagc tcgctacccg gatcgatgat
    39001 gccgagccta aggtggttct gagcgcgtcg tgcgggatcg agttcgccaa cgtcatcccg
    39061 tatcaacctc tcgtagacga agccatttcc cttgccgtcc acaaaccgga acgcgtggtg
    39121 atgctgcagc gcgagcagca agaagcggat ctttccggcg agcgttatct ggactgggcc
    39181 agctgggaag cctcggccca gggtgtcggc tgcgtgccgc tcgatgccac cgatccgctc
    39241 tacgtccttt acacgtcggg caccaccgga aagcccaagg gtgtggtgcg ggacaacggc
    39301 ggccacgcgg tggcgctgaa ctacagcatg gaagccatct acggcatgga cgcgggcgac
    39361 gttttctggg ccgcgtcgga cgtcggctgg gtggtgggcc attcctacat cgtctacgga
    39421 cccctcatcc gtggctgcac ctcggtcctg ttcgaaggga agcccgtgcg gacccccgac
    39481 gcgggcgttt tctggcgcgt gattcaggat caccgggtga agtgcttctt cgtcgcgcca
    39541 acggcctttc gcgcgattcg caaggaggac cccgactgcg cgctgaagga tcgttacgac
    39601 gtctcctccc tgaagtacca gttcgtcgcc ggcgagcgcc tcgacccgcc cacctatcac
    39661 tggctgagcg agcacctgca cgtccctgtg atcgaccact ggtggcagac ggaaacgggc
    39721 tgggcaatgt gtgcgaacat ggccggtctg gagctcaagg acgcgaaacc gggctcgccg
    39781 accctccccg ttccggggta cgacctgcgc attctcgatg aagacggcgt cgaggtgcca
    39841 gccggcgtgg aaggggcggt cgtggtcagg gagcccctgc cgcccggcgc tttcccaacg
    39901 gtgtggggcg atcatcagcg ctacgtcgat tcctactggt cccgctatcc cggcttctac
    39961 ctgaccggcg acgagggcta tcgcgacgac gaaggttacg tctacatcat gggccgcatg
    40021 gacgacgtca tcaacgtagc cggccaccgg ctctccaccg ggcagatgga ggagacggtc
    40081 gccgagcatc ccgcggtggc cgagtgcgcc gtggtcggca ttgcggatgc ggacaaaggc
    40141 caggttcccg tcggtctggt gctggtcaag gacggtgtca acgtcgatcc tcaagcgctt
    40201 gaagccgagc tcgtgcagat ggttcgggca tcggtgggcg cttttgccaa cttccggcgg
    40261 gcggtggtcg tggagcgtct gcccaagacg cgctcgggaa agatcctgcg gcaggtgctg
    40321 cgaaagatga tcgatggcaa tccctacagc gttccctcca ccatcgacga tcctgcgatc
    40381 atcccggaga tggcctcgac tttgaagaaa gcgggggtga tgtgaccggg ctgctgctaa
    40441 aatcggcaac aggcagatga tcatcgttca cggcaccatc ccaatccgtc cggatcagcg
    40501 cgaacgcgct ctggagctcg cccgccggat gagcgaggca acccaggcag agctcggctg
    40561 catctcctac gacttctacg tggggctgtc cgatcccaac acgctgatgc tttttcagga
    40621 atgggagacc atggaagcgc tgatggctca cttccagacc gagcacatgg acgacttcct
    40681 ccgagaactt ccgcaagtgg tggcggggga gatcacgacc cggcgctatg ccgtgcagag
    40741 cgttgacgaa gacaccgagg agacgccaga gacgccgccc atcgttcact gagacgcagc
    40801 ttgccctacc gtgcgccggg cggcgtagct cgccgcgatg agcaggccga cggcaaagcc
    40861 cgtttcccac aggcccagcg ccctgaggtc ggccgtggcg gccagcagca cgccgtgtat
    40921 gaagtaaagg gtgctcacca gcgcggcgaa gacgtagctt cggtagcggt tcctcagcgt
    40981 gcctggaagc acggccagca ggggcagcac ctggatcaga aaccagatca ggttcacatg
    41041 gggcgtggcc agcggctcca cgaagaactg gcgcagcccg ttgatcgcga agaggctgcc
    41101 catcatcagc aggcacgcga gctgcagtcc tttcacgcgc cgagcctacg ggcgagctcg
    41161 gccaacctgc gccccagggc catggcgatt gcctgctcgt ccgccgttgg ctcggtcccc
    41221 gcctcgccac cgctgacgtg gctggggccg tatggcccac cgc
//
LOCUS       DBNHLM010000043        10369 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1383109, whole genome shotgun sequence.
ACCESSION   DBNHLM010000043 DBNHLM010000000
VERSION     DBNHLM010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10369)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10369)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10369
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1383109"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="AC16QF_02300"
     CDS             complement(<1..929)
                     /locus_tag="AC16QF_02300"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR6447414.1"
                     /translation="MNFFRAQDDAKRKTWRLALLFAAAVGSLIVLTNLLLMGVYRWTT
                     DVGVATSPDATAAFTNVPTEQWLIVTAAVVAVVGIASLYKYLVIRDGGKTIAEALGGR
                     RINANTTDPAERRLLNVVEEMAIASGTAVPPVYLIPEHSINAFAAGFTPDDAVIGINQ
                     GTLDQLNRDELQGVVGHEFSHILNGDSRINLRLIAMLHGILFIGIIGYGILRGMAHGG
                     NRRSGGNAAVPVMALGLGLLIIGYSGTFFGNLIKAAVSRQREYLADAAAVQFTRNPSG
                     LANALKRIGGLPVGSTMSGSNAREISHMFFGQCS"
     gene            complement(946..1539)
                     /locus_tag="AC16QF_02305"
     CDS             complement(946..1539)
                     /locus_tag="AC16QF_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009043301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR6447415.1"
                     /translation="METIIVLVIIAALAFWVIAIYNRLVTLKNRFKNAFSQIEVQLKR
                     RYDLIPNLVETAKAYMSHERETLEAVIAARNQALAGLQTAAQDPGNADAIRQLASAEG
                     ALGGALGRLNVVMEAYPDLKANQNMMQVSEELTSTENRVAFARQAYNDQVTDYNTYKQ
                     SFPAMLLAGPFGHAGDAALLEYEDSEAMQEAPKVSFS"
     gene            complement(1582..2775)
                     /locus_tag="AC16QF_02310"
     CDS             complement(1582..2775)
                     /locus_tag="AC16QF_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6447416.1"
                     /translation="MTDLNEFRKEVRNWLEENCPPSMRTSTPEGETVWGGRREKFANP
                     DSRIWLDRMAERGWTAPTWPREYGGGGLTADEDKVLQQELRSINARPALQSFGIWMLG
                     PALLEFASEEQKLEHIPQIVRGEIRWCQGYSEPGYGSDLAGLQTKAEDQGDHFLVNGS
                     KIWTSYADVADWIFCLVRTDNDAPKHEGISFLLFDMDSEGVTTSPIELISGASPFCQT
                     FFDNVRVPKANLVGGLNKGWSIAKRLLQHERQMVAGIGGMSALGGTGRRLEDVAKAYV
                     GEAQGRIGDPAVRSSVIEHRMNDRAFQLTLERSGDEAKAGQADGNLASMFKYYGTEQN
                     KRKYELMLQAMGTQSMGWEGEAFSAKELGTTRQWLRSKANSIEGGTSEVQLNVIAKRV
                     LGLPD"
     gene            2898..3383
                     /locus_tag="AC16QF_02315"
     CDS             2898..3383
                     /locus_tag="AC16QF_02315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR6447417.1"
                     /translation="MSEIDQRVFRDTMGQFCTGVVIATGCREGEPAGFAAQSFSSVSL
                     DPPLVVLCPAKTSTSWPKLRDSGSFCINILGEDQKAVCDVFAQSGVEKFASLDWSPGA
                     TGSPVLADVIAYVECDLHAEHDAGDHTLAVGLVREVSIVNPDKPPLLFFRGGYGQFEG
                     L"
     gene            3383..4504
                     /locus_tag="AC16QF_02320"
     CDS             3383..4504
                     /locus_tag="AC16QF_02320"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR6447418.1"
                     /translation="MNLKTRVCEVLEIEHPIVLAGMGGASCPALAAAVSNAGGLGVLG
                     AAGCGPELLRRWIRETRELTDRPFGVDTLLPASVRRANYDETEGPTPMDLLPETQAYA
                     AEFMEREGLEPVASESPRPPRTSDEPAVFTKDFFEAQMRVVIEERVPVYASGLGNPGP
                     WMDELRANGTKVMAVVGAVRHAIQVKSSGLDIIVAQGHDGGGHNSPIGTMALIPQVVD
                     AVGDTPVLGAGGIVDGRGIAAAMMLGAEGVWIGSAFLASQEANIFDFQKQAIVDATEE
                     GTSVSRSVTGKPARIIRSKWTDYWARGERDPLPMPFQGLVSQPVLAAANRDGRGDINP
                     GFAGQGIGMIKAVRPAREIFDELVYGAEQALARTSNLLR"
     gene            4613..5092
                     /locus_tag="AC16QF_02325"
     CDS             4613..5092
                     /locus_tag="AC16QF_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6447419.1"
                     /translation="MSDTETNMNNALEILRQRLDQPNPSSDWFEITQDRVNDYADVSM
                     DHQWIHVDVERAKQGPFGAPIAHGNLTIAVMGHLPRSETASQPPLEGHKLSINYGFDR
                     IRFPAPVRVGDRIRATSTPRRAEIKGGMIETMNEIVVEIEGQEKPALVAESLGRLVF"
     gene            complement(5145..6242)
                     /locus_tag="AC16QF_02330"
     CDS             complement(5145..6242)
                     /locus_tag="AC16QF_02330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024151.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6447420.1"
                     /translation="MTSYDPFLRGPHPVGVRTFHWTDKTRQRTLPVEVWYPADDEHAG
                     QDLDPDTQDHFQPMSLSPPVPQAAVRDAAPAEGEPRPLLIFSHGFGGTGRQTTHLCTH
                     WASHGYVVAAMDHVGNTTEDVMAAAAQPVDPGTLLERLKSFAADRPVDVSFVIDRMVA
                     GDAGLTVDPDRVGVSGHSFGGWTTLASAGMDPRIRAALPLAPAGGQSSQWQSSIADRL
                     ADALAMSWEREVPTLFLVAEHDVVLPLDSMHDLIERTPHPRRAVNLLNADHFHFCDRV
                     EQTHDMVRMMGQMIFGTRSEADQEAPSALAAMKPSSELCPGDHAYALLQGLGLAHMDA
                     HVRELDRAHALLAGDLASLMAERGVAVKVMA"
     gene            6517..8259
                     /locus_tag="AC16QF_02335"
     CDS             6517..8259
                     /locus_tag="AC16QF_02335"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6447421.1"
                     /translation="MVNRRRFVEAGALTAAGLSLWGCGADTRPRSAGAGDGQVAGPGR
                     AVVFRSGVVLPVDAGFSEQRAIAIRGNRILAVGDDDDVLAAAGKDPQVVDLDGRVLLP
                     GFIEPHMHFALMAGLGHWPDIGPFKHESTEDALAALRLIARETPADEWIVARQFDPSL
                     QTGPEMLTTRELDTVSTDRPVFVLNASGHLAYGNSRLLEFAGIDRDTPDPPGGEYFRD
                     PDGRPNGAMSQVAYQPVMMRNEALIAVLGSGFVEAGLRVGEEAAALGITTLCDQATGG
                     IAGMGDIDFYRQMFASSRMSARIRASLYSETAAEWDDNGVAFGDGDALMRIVGWKIVT
                     DGSNQGFTGRQREPYLDSDNRGMYYVAPEALNEMVLSRGRRGWPLVLHGNGDAAIDSI
                     LDAVQAAADAGVDVGKLRCRIEHASILHDGQIQRIRELGMGPSFLINHVHYWGHAMRD
                     KVFGPEKVQLLDRCRSVEDAGITWTMHSDAPVSPLGTLHKIRVAVARDLWKEPDTTLA
                     PHERVSVEAAIRAVTINAAWQCHSETEVGSLAEGKLADLVVLEDDPRKVEPKAISEIG
                     VSQTWMDGNQVFGA"
     gene            complement(8294..8977)
                     /locus_tag="AC16QF_02340"
     CDS             complement(8294..8977)
                     /locus_tag="AC16QF_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6447422.1"
                     /translation="MSSDYTGNVVVVTGAAGALGRAVVEHFAASGATVAQLDVVAIDN
                     AHFSDTCDLTDPDSCRQAVTRIIETLGRIDVLANIAGGFTMGEAVHETSEETWDFMMG
                     LNARSVLNMARAVVPIMLQQGAGKIVNVGAGAGLAGVANMGAYSASKSVVIRLTEAMS
                     AELKERRINVNCVLPSIIDTERNRADMPDADFHRWVTPAAMAGVIGFLASPAADPIHG
                     AAVPVSGLS"
     gene            complement(8974..9846)
                     /locus_tag="AC16QF_02345"
     CDS             complement(8974..9846)
                     /locus_tag="AC16QF_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6447423.1"
                     /translation="MKLGKLGVWYFLDGLPADAAAAAAQRIEELGYSALWIPEATGRN
                     PMVHASWLLANTKQLIVATGIANIYHREPGVTVSAQKALAEQSGDRFLLGIGVSHKPF
                     VEGVRGLTYGPPVASMRDYLDKMAAAPYTSVPPAEDPPTVIAALGPKMLALAAEKTAG
                     AHPYFVSPDHTAMASEVLGPDKWLCVEQKVILETDPSRARELARPAAQMYLALPNYRN
                     NWLRMGFTEADLGNGGSDRFIDATFAWGSAAQVEARIREHFDAGATHVCIQPVNPNGQ
                     FGDLHWEALEALAP"
     gene            complement(9848..>10369)
                     /locus_tag="AC16QF_02350"
     CDS             complement(9848..>10369)
                     /locus_tag="AC16QF_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6447424.1"
                     /translation="REAARLFGSKKPVIGAIQGAAIGGGLGLSLVPDFRVACPEARFG
                     ANFVKLGIHQGFGISVSLPRIIGQQAASMLLLTGRRIGGEEAFKLGLVDVLTDAESLR
                     DEAMKLAQEIAENAPLAVLSVRGTMRAGLAEAVIEATEHELKEQQWLRATEDAQEGIR
                     SVAERRPGNFQGR"
BASE COUNT         1649 a         3370 c         3527 g         1823 t
ORIGIN      
        1 gagcactggc cgaagaacat gtggctgatc tcacgggcat tcgatcccga catggtcgac
       61 cccacgggca ggccgccgat gcgcttgagc gcattggcca ggccgctggg gtttcgcgtg
      121 aactgcaccg ccgcagcatc tgccagatac tcgcgctgtc ggctcaccgc ggccttgatg
      181 agattgccga agaaggtgcc cgagtagccg atgatcagca gccccaggcc gagggccatc
      241 accggcaccg ccgcgttgcc cccggatcgg cggttgccgc cgtgggccat gccccggagg
      301 atgccgtaac cgatgatgcc gatgaagagg atgccgtgca gcatcgcgat caggcgcagg
      361 ttgatgcggc tgtcgccgtt caagatgtgg ctgaactcgt ggccgaccac cccctgaagc
      421 tcgtccctgt tcagctggtc aagggtgccc tggttgatcc cgatcacggc gtcatcgggc
      481 gtgaatccgg ccgcgaaggc gttgatgctg tgctccggaa tgagatagac ggggggcacg
      541 gcggtgccgg aggcgatggc catctcctcg accacgttga gcaggcgccg ttcggccgga
      601 tcggtggtgt tggcgttgat ccggcggcct ccgagcgcct ctgcaatggt cttccctccg
      661 tcgcgaatga ccaggtactt gtacaggctc gctatcccca ccacggcgac cacggctgcg
      721 gtgacgatga gccactgctc ggtgggcacg ttggtgaagg ccgcggtagc gtccggcgag
      781 gtggccactc cgacgtccgt ggtccagcgg tagacgccca tcagcaggag gttcgtgagc
      841 acgatcagcg agcccacggc ggccgcgaac agcagcgcca ggcgccaggt cttgcgcttc
      901 gcgtcgtcct gggcgcgaaa gaagttcatc cgaccccggg gacgatcagc tgaaggaaac
      961 cttcggggcc tcctgcattg cctcgctgtc ctcgtactcc agcagggccg cgtcgccagc
     1021 gtgaccgaag ggcccggcga gcagcatcgc aggaaagctc tgcttgtagg tgttgtagtc
     1081 ggtcacctgg tcgttgtacg cctgccgggc gaacgccacg cgattctcgg tgctcgtcag
     1141 ctcctcggaa acctgcatca tgttctggtt cgccttgagg tcggggtagg cctccatcac
     1201 cacgttcagc cgccccagcg cgccgcccag cgccccttcg gcgcttgcca gctgacgaat
     1261 cgcatcggcg ttgccagggt cctgagcggc cgtctgcagg ccggccaggg cctggttgcg
     1321 ggcggcgatc accgcttcga gggtctcccg ctcgtgtgac atgtacgcct tggcggtctc
     1381 gaccaggttc gggatgaggt cgtagcgccg cttgagctgc acctcgatct gcgagaaggc
     1441 gttcttgaag cggttcttca gggtcaccag ccggttgtag atggcgatca cccagaaagc
     1501 gagcgccgct atgatgacga gcacgatgat ggtttccacg atctcctcct tggccggatg
     1561 tcggtccggg aactcccgcg ttcagtccgg cagccccagt actcgctttg cgatcacgtt
     1621 gagctgcacc tcgctggttc ccccctcgat ggagttcgcc ttggagcgca gccattgccg
     1681 cgtggtgccg agctctttgg ctgagaaagc ctcgccctcc caacccatgc tctgggtgcc
     1741 catggcctgc agcatcagct cgtactttcg tttgttctgc tcggtgccat agtacttgaa
     1801 catgctggcc agattgccgt ccgcctgccc cgccttggct tcgtccccgc tgcgctccag
     1861 agtcagctga aaggctcggt cgttcatgcg gtgctcgatg acgctgctgc ggacggccgg
     1921 gtccccgatg cgcccctgtg cctcgccgac gtacgccttg gccacgtcct cgagccggcg
     1981 gccggtgcct ccgagggcgc tcatcccgcc gatcccggcc accatctggc gctcgtgctg
     2041 cagcagccgc ttggcgatgc tccagccctt gttgagcccg cccaccaggt ttgccttggg
     2101 caccctcacg ttgtcgaaga acgtctggca gaagggcgag gcgccgctga tcagctcgat
     2161 gggggaggtg gtcacgccct cggagtccat gtcgaagagg agaaagctga tgccctcgtg
     2221 cttcggcgcg tcgttgtcgg tgcggaccag gcagaagatc cagtccgcaa cgtccgcgta
     2281 ggaggtccag atcttggaac cgttgaccag gaagtggtcg ccctggtcct ccgccttggt
     2341 ctgcagcccg gccaggtccg agccgtagcc cggctccgag tagccctggc accagcggat
     2401 ctcgccgcga acgatctgcg gtatgtgctc gagtttctgc tcctcgctgg cgaactccag
     2461 cagggccggc cccagcatcc agatgccgaa gctctgcagc gccggcctgg cattgatgct
     2521 gcgcagttcc tgctggagca ccttgtcctc gtcggccgta aggccgccgc cgccgtactc
     2581 ccggggccac gtgggtgccg tccacccgcg ctcggccatg cggtccagcc agatcctgga
     2641 gtccggattc gcgaacttct cccggcggcc gccccagacg gtctcgccct cgggcgtgga
     2701 cgtgcgcatg ctgggcgggc agttctcctc tagccagttt ctgacctctt tgcgaaactc
     2761 gttaagatct gtcaccgctg tctgaacctc tgctgcgccc tatctgccgg actcggaatt
     2821 ctaaaggaaa cgggctcgtt ggccagggca gcagcggaag gttatagtcc gcggctcttc
     2881 gatcccaggg gagcgggatg tcggaaatcg accaacgggt gtttcgcgac accatgggac
     2941 agttctgcac gggggtggtg attgcgacgg gctgccgaga gggcgagccg gccggtttcg
     3001 ccgcccagtc cttcagctcg gtttcgctgg acccgccgct ggtcgtgctg tgccccgcca
     3061 agacgagcac cagctggccg aagctcaggg actcgggcag cttctgcatc aacattctcg
     3121 gcgaggatca gaaggccgtc tgcgacgttt tcgcgcaatc gggggtcgaa aagtttgcca
     3181 gcctcgactg gtcgccgggg gcaacgggat cccccgttct ggccgacgtg atcgcctacg
     3241 tcgaatgcga cctgcatgcc gagcacgatg cgggcgacca tacgcttgcc gtcgggctgg
     3301 tgcgtgaggt cagcatcgtc aaccccgaca agccgccgct cctgttcttc cgcggcggct
     3361 atggtcaatt cgagggactg tgatgaatct gaagacgcgc gtctgcgaag tgctagaaat
     3421 cgagcacccc atcgttctcg caggaatggg cggggcgagc tgcccggccc tggccgcggc
     3481 ggtatccaac gccggcgggc tcggtgtgct gggagccgcg ggatgtggtc cggagctcct
     3541 gcggcgctgg atccgcgaga cgcgcgagct caccgacaga cccttcggtg tcgacacgct
     3601 gctgcctgcg tcggttcggc gcgccaacta cgacgagacc gaggggccga cgcccatgga
     3661 tctgctgcct gagacccagg cctacgcggc tgagttcatg gaacgcgaag gcctggagcc
     3721 ggtggcgagc gagtcgccgc ggccgccgcg aacgtccgac gagcccgccg tgttcacgaa
     3781 agatttcttc gaggcgcaga tgcgcgtggt catcgaggag cgggtccccg tttacgcgtc
     3841 cggtctgggc aaccccggac cgtggatgga cgagctgcgc gccaacggca ccaaggtgat
     3901 ggccgtggtg ggcgccgtcc ggcacgcgat tcaggtgaag tcctccgggc tcgacatcat
     3961 cgtggcccag ggtcacgacg gcggcggtca caattcgccc atcggcacca tggcgctgat
     4021 accccaggtg gtggacgcgg tgggcgatac gccggtgctg ggcgccggtg gcatcgtcga
     4081 cggccgaggc attgctgcag ccatgatgct cggagccgag ggggtctgga tcggctccgc
     4141 gtttctcgcg tcccaggaag ccaacatctt cgattttcag aagcaggcca tcgtcgatgc
     4201 caccgaggag ggaacctccg tgtcccggtc cgtgacgggt aagccggcac ggatcattcg
     4261 cagcaaatgg accgattact gggcgcgggg cgagcgggat cccctgccga tgcccttcca
     4321 gggcttggtc tcccagccgg tgctggccgc ggcgaaccgc gatggccggg gcgacatcaa
     4381 cccgggtttc gccggccagg gcatcggcat gataaaggcc gtccgcccgg cccgggaaat
     4441 attcgacgag ctggtttacg gggccgagca ggcgctcgct cgaacgtcga acctgcttcg
     4501 ctgagcggcg ccggcccgat cgtggacctc atcggctccc tcgatgcgaa cgacgatgcc
     4561 agccggtggg ccccgcccgt ttgatttttg acgattaccg gagaacgaga tcatgtccga
     4621 caccgaaacc aacatgaaca acgccctgga gattctgcga cagcgtctcg accagcctaa
     4681 cccctcctca gactggttcg agatcactca ggatcgggtc aacgactatg ccgacgtcag
     4741 catggatcac cagtggatcc acgtcgacgt cgagcgggcc aagcaggggc cgttcggtgc
     4801 gcccatcgcc cacggcaacc tgaccatcgc cgtgatgggc cacctgccgc gatctgagac
     4861 ggcctcccag ccaccgctgg agggccacaa gctcagcatc aactacggct tcgaccgtat
     4921 ccgttttccc gcgcccgtgc gggtgggcga caggatccgc gccaccagca cgccgcgccg
     4981 cgccgagatc aagggcggca tgatcgagac catgaacgag atcgtggtcg agatcgaggg
     5041 tcaggaaaag ccggcgctgg tggccgaaag cctgggtcgc ctcgtcttct gatctgccgg
     5101 gccgtcaccc ggacggggga acggggggtg ccggcgtgcg ctgctcaggc catgaccttg
     5161 accgccacgc cccgttcggc catcagcgac gccaggtcgc ccgccagcag cgcgtgggcc
     5221 cggtcgagct ccctcacgtg ggcgtccata tgggccaggc ccagcccctg aagcagcgcg
     5281 taggcgtgat cgccagggca cagctcgctg ctcggcttca tggccgccag cgcggagggc
     5341 gcttcctggt ccgcttcgct gcgcgtgccg aagatcatct gtcccatcat cctcaccatg
     5401 tcgtgggtct gctccacccg atcgcagaag tggaagtgat cggcgttcag tagattcacg
     5461 gcgcggcgcg ggtgcggcgt tcgctcgatg aggtcgtgca tgctgtccag cggcaggacc
     5521 acgtcgtgct cggcgaccag aaacagcgtg ggtacctcgc gctcccagct catggcgagc
     5581 gcgtctgcca agcggtcggc gatgctcgac tgccactggc tcgattggcc cccggccggt
     5641 gccaggggca aggcggcgcg gatgcgtgga tccattccgg ccgatgccag ggtggtccag
     5701 ccgccgaagc tgtgcccgga gacgccaacg cggtcgggat cgacggtcag gccggcatcg
     5761 ccggccacca tccggtcgat cacgaagctg acgtccactg gccggtctgc ggcaaacgat
     5821 ttcaggcgct cgagcagggt ccctggatcc actggctggg cggccgccgc catcacgtcc
     5881 tccgtggtgt tgccgacgtg gtccatggcg gcgaccacgt agccgtgact ggcccaatgg
     5941 gtgcacaggt gtgtcgtctg gcggccggtc ccgccgaagc catgagagaa gatcagcagg
     6001 ggccggggct caccctcggc cggtgccgcg tcgcgaacgg ctgcctgggg taccggtggc
     6061 gacaggctca tgggctggaa gtgatcctgg gtatccgggt cgagatcctg cccggcgtgc
     6121 tcgtcgtcgg ccgggtacca cacttccacg ggcaacgttc gttgccttgt tttgtcggtc
     6181 cagtgaaagg tgcgcacgcc gaccggatgc gggccacgca ggaacggatc atacgatgtc
     6241 atcgtctacc tctgtgtcag aacggttgag cgtcgggctc gcagtctggc agcgtggtgc
     6301 tgcccgaatc gaggctggct cgactctcga gatctcgacg cgccgcgaag gataccgaga
     6361 ggattccatt gccgccagca tgagccgtgc atgtatcggg ttgcttcgtt tcggctggct
     6421 cgccgtgtac gctgacgcag caaaggagac cgtggcccgt tggcggcagg actcaccgga
     6481 agtcgaggga agaacagatt ttggaggccc gccgggatgg ttaacagaag acgattcgta
     6541 gaggccggcg ccctgacggc tgccggcttg tcgctgtggg gttgcggtgc tgatacccgt
     6601 ccgaggtctg ccggggccgg cgacgggcag gttgcgggtc ccggccgcgc cgtcgttttc
     6661 cgcagcggcg tggtgttgcc ggtggatgcg ggattctccg agcagagggc gattgccatc
     6721 cgcggaaacc gcatccttgc cgtcggggat gacgacgacg tgctggcggc ggcggggaaa
     6781 gacccccagg tggtcgatct cgatggccgg gtcctcctgc cgggattcat cgaaccccac
     6841 atgcacttcg cgctgatggc cgggctgggc cactggcctg acatcggccc gttcaaacac
     6901 gaaagcaccg aggacgcgct cgccgctctg cgcctcattg ccagggagac gccggctgat
     6961 gagtggatcg ttgcccgtca gttcgatccc agccttcaga cgggtcccga gatgctgacg
     7021 acccgggagc tagataccgt gtcaacagac cggcccgtct ttgtgctgaa cgcctcgggc
     7081 catctcgcct acggtaactc ccgcctgctg gagttcgctg gcatcgacag ggacacgccg
     7141 gatccgcccg gcggggagta tttccgtgac cccgatgggc ggcccaacgg cgccatgtcc
     7201 caggtcgcct accagccggt gatgatgcgg aacgaggcgc tcatcgcggt tcttggctcc
     7261 gggttcgttg aggccgggtt gcgggtcggc gaagaggcgg ccgcgctcgg gatcaccacc
     7321 ctctgtgacc aggccaccgg gggcattgcc ggcatgggcg atatcgactt ctaccggcag
     7381 atgtttgcct cgagccggat gagcgcgcgg attcgcgcct cgctctattc cgagacggcc
     7441 gcggagtggg acgacaacgg cgttgccttc ggtgacgggg acgccctgat gcgcattgtg
     7501 ggctggaaga tcgtgaccga tgggtccaac cagggtttca cgggccggca gcgtgagccg
     7561 tatctagact cggacaatcg gggcatgtac tacgtcgcgc ccgaagcgct gaatgaaatg
     7621 gtgctcagcc gtggccgtcg aggctggccg ctggtgctgc acggcaacgg cgacgccgcg
     7681 atcgacagca ttctggacgc ggtgcaggcg gcggcggatg cgggcgtgga cgtgggaaag
     7741 ctgcgctgcc gcatcgagca cgcgtcgatc ctccacgatg ggcagatcca gcgcattcgc
     7801 gagctgggca tgggccccag ctttctcatc aaccacgtgc actattgggg tcacgccatg
     7861 cgcgacaagg tgttcggccc ggagaaagtg cagctgctcg atcgctgccg gagcgtcgaa
     7921 gatgccggca tcacttggac gatgcacagc gacgcgccgg tgtcaccgct tggcaccctg
     7981 cacaagatcc gggttgcggt ggcccgcgac ctctggaagg agcccgacac cacacttgcg
     8041 ccccacgagc gcgtttccgt ggaggcggcg ataagggccg tcaccatcaa cgcggcatgg
     8101 cagtgtcact cggagaccga ggtcggcagc ctagcggaag gcaagctggc cgatctggtc
     8161 gtgctggagg acgacccgag gaaggtcgag cccaaggcca tctccgagat cggggtgagc
     8221 cagacgtgga tggacgggaa ccaggtattt ggagcctgat ccgcagcgtc gcgggccggt
     8281 cggcccgcgc ccctcagctc agcccgctga cgggcacggc ggcgccgtga atggggtccg
     8341 ctgcaggtga cgccagaaag ccgatgaccc cggccatggc cgcgggcgtc acccaacggt
     8401 ggaaatcggc gtcgggcatg tcggcccggt tgcgctcggt atcgatgatg gacggcagca
     8461 cgcagttgac gttgatgcgt cgctccttga gctccgcgga catggcttcc gtcaatcgga
     8521 tgaccacgct cttagacgcg ctgtaggcac ccatgtttgc gactccggcg agccctgcgc
     8581 cggcgccgac gttgacgatc ttgccggccc cctgctgaag catgatgggg accacggccc
     8641 gggccatgtt cagcaccgag cgcgcgttca ggcccatcat gaaatcccag gtctcctcgg
     8701 aggtttcgtg aacggcctct cccatggtga agccgccggc gatgttggcc agcacgtcga
     8761 tacgccccag cgtctcgatt atccgggtga ccgcctgcct gcagctgtcc gggtcggtca
     8821 gatcacaggt gtcggagaag tgggcgttgt cgatggccac cacgtctagc tgcgccacgg
     8881 tggcgccgct tgcggcgaag tgctcgacca cggcacggcc cagggcgccg gccgcgcccg
     8941 tgacgacgac tacgttgcct gtatagtcgc tgctcatggc gcgagcgcct ccagggcttc
     9001 ccagtgtaga tcgccaaact gaccgttggg attgacgggc tggatgcaga cgtgggtcgc
     9061 gccggcatca aaatgctcgc gaatgcgggc ttcaacctgg gccgcgctgc cccaggcaaa
     9121 ggtggcgtcg atgaaccgat cggaaccgcc gttgccgagg tcggcttcgg tgaagcccat
     9181 gcgcaaccag ttgtttcggt agttgggcag ggccaggtac atctgcgccg ccgggcgggc
     9241 gagctcccgg gcccgggaag gatccgtctc cagaatgact ttctgctcta cgcacagcca
     9301 cttgtccgga ccgaggactt cgctggccat ggcggtgtgg tcgggcgaaa cgaagtaggg
     9361 gtgggcgcct gcagtcttct cggcggccag ggccagcatc ttcgggccca gcgcggcgat
     9421 caccgtgggc gggtcttcgg ccggcggaac gctcgtgtag ggcgcagcgg ccatcttgtc
     9481 cagatagtcc cgcatgctcg ccacgggcgg gccgtaagtc aggccgcgca cgccctccac
     9541 gaagggcttg tgagaaacac caattccgag caggaaccgg tcgcccgact gctcggccag
     9601 ggccttttgc gccgagaccg tgacgccggg ttcccggtgg tagatgttgg cgattcccgt
     9661 ggccacgatc agctgcttgg tgttggccag cagccacgag gcgtgaacca tggggttgcg
     9721 ccccgtcgcc tccggtatcc acagagcgct gtaacccagt tcctcgatgc gctgagcggc
     9781 ggcggccgcg gcatccgccg gcaggccgtc gagaaagtac cagacgccga gcttgccgag
     9841 cttcacctca acggccctgg aagttgccgg gccgacgctc ggccacggag cggatgcctt
     9901 cctgggcgtc ctcggtggcg cgcagccatt gctgctcctt gagctcgtgc tcggtggcct
     9961 cgatgacggc ctcagcgagc ccggcgcgca tggtgcccct gactgacagg acggccagcg
    10021 gggcgttctc ggcgatttcc tgcgccagct tcatcgcctc gtcgcgcagg ctctcggcgt
    10081 cggtcagtac gtccaccagg ccgagcttga acgcctcttc gccgccgatg cgccggcctg
    10141 tcagcagcag catgcttgca gcctgctgac cgatgatgcg gggcaggctg acggagatgc
    10201 cgaagccctg gtgaatgccg agcttgacga agttggctcc gaatcgcgcc tccggacagg
    10261 cgacgcgaaa atccgggacc agggacagac ccaggcctcc gccgatggcg gcgccttgga
    10321 tcgcgccgat taccggtttc ttgctgccga acagccgcgc ggcctcgcg
//
LOCUS       DBNHLM010000044         3271 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_425358, whole genome shotgun sequence.
ACCESSION   DBNHLM010000044 DBNHLM010000000
VERSION     DBNHLM010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_425358"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="AC16QF_02355"
     CDS             complement(<1..485)
                     /locus_tag="AC16QF_02355"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6447425.1"
                     /translation="MLRRVAASLRRPVASLCAILFVSGPALAAEIEEIVVTARGVEQS
                     VRDIPVAISVVSEERMANLNLKSLEDIAEVTPQLNIVRASSGSGTSISIRGIGSSPST
                     IGIEQSVSTIVDGVYYPQGRVINEAMFDVRQVAILKGPQALYFGKNATAGVISVETNN
                     PG"
     gene            complement(643..1170)
                     /locus_tag="AC16QF_02360"
     CDS             complement(643..1170)
                     /locus_tag="AC16QF_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6447426.1"
                     /translation="MSDRVSSYRFFQEAYLVNDLDQAIRGWSDLYGAGPFRTVMHHKT
                     DRFHYRGTDQEADVSYAFGYLGDMQIQFIEQHDDAPSIYRDMYPAGEEGFHHVAILVH
                     DFEAEYERLVGKGFTDACRLFADGVDAAYFDTRSVNGCFTEIHGDPPRNLGRWAQMRR
                     AHELWRPGDDVYMTL"
     gene            1272..2372
                     /locus_tag="AC16QF_02365"
     CDS             1272..2372
                     /locus_tag="AC16QF_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6447427.1"
                     /translation="MAGTSVADSALRQWLDIQSIRNVLAIHSRGIDRADGDLLRSAYH
                     PDAEVAYGFFDGPAAELCEIMGNPSEQITMHRTSNMWIRLLDDDHAISESYAFVYSES
                     ADDAGYGQALIGGRYLDRHEKRGGEWRISHRTYVLEWNANWPGTGTVLPDYESPINRR
                     GSKTPDDLGTRLLNESQQILTSGDATMTIPEDLEAAVADALARQDIHDLIMAQARATD
                     RRDRALLESVFHEGATMDAGGLFDGPAPEYCDFILEMTAPLKKMYHSVANEWVRVEGD
                     SGVAESYVIAFTTAADEAGGDVDNLSGGRYLDRFERRDGVWKFTHRAYVMDWTMHQPT
                     TDQSDDPDSMYASLTNRGGHWPDDPVYGLWGK"
     gene            2376..>3271
                     /locus_tag="AC16QF_02370"
     CDS             2376..>3271
                     /locus_tag="AC16QF_02370"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6447428.1"
                     /translation="MSETSDRPAPGEARMPGPSYRDFILRDRAAPPAAFLEESYEFQG
                     DEDIPYSNYTSHDHAQAEFEKMWPRVWQWACREEHIPNPGDFHVYDVGDLSAIVVRTP
                     SGAVKAYFNACMHRGTQLKPPGMTGSSEDLRCPFHGWTWSLDGDLVDLPQAWDFPHVT
                     EDSHRLPEVKVGLWGGFVFVNFDPDAAPLEDYLGVLPRHFADFHIEDRYIELHIRKRL
                     PANWKASAEAFLEAYHVRETHAGGRPNTEVATQYDVFGDNVTRFIHTVGAPCPLVDPP
                     PSEQQLLEMLWVRGREDEPVPQV"
BASE COUNT          583 a         1064 c         1074 g          550 t
ORIGIN      
        1 ccggggttgt tggtctccac ggagatgacg ccggccgtgg cgttcttgcc gaagtacagc
       61 gcctgggggc ctttcaggat ggccacctga cggacgtcga acatggcctc gttgatcacc
      121 cggccctggg ggtagtaaac gccgtcgacg atggtggaca cggactgctc gatgccgatg
      181 gtgctcggcg aggagccgat gccgcggatg ctgatgctcg tgccgctgcc ggagctggcg
      241 cggacgatgt tgagctgggg cgtcacctcg gcgatgtcct ccaggctctt cagattgaga
      301 ttggccatgc gctcctcgct gaccacggaa atggccaccg ggatgtccct gacgctctgc
      361 tcgacacccc gggcggtgac gacgatctcc tcgatttcgg ccgccagagc ggggccggac
      421 acaaacaaga tggcgcaaag tgacgccacc gggcgtcgca gagacgccgc aactcttctc
      481 agcattttaa tctccccaag aacggatcgg attccgaacg agcggggttt taacccgaac
      541 cgggtttcag cgcaaccggc cgttccggtt tcaggcgttc atgcgaacgt cgcccacaaa
      601 ggcggggccg tccggccggt tcgaaaacgc cgccccgccg cgctacagcg tcatgtagac
      661 gtcgtcaccc ggccgccaga gctcgtgggc gcggcgcatc tgcgcccagc ggccaagatt
      721 gcggggcggg tcgccatgaa tctccgtgaa gcagccgttg accgaccggg tatcgaagta
      781 ggcggcgtcc acgccgtcgg caaagagccg gcacgcgtcg gtgaacccct tcccgaccag
      841 gcgttcgtac tcggcctcga agtcgtggac gaggatggca acgtggtgaa agccttcctc
      901 gccggccggg tacatgtcgc ggtagatgga gggggcgtcg tcgtgctgct cgatgaactg
      961 aatctgcatg tcgcccaggt agccgaacgc gtaggagacg tcggcttcct gatccgtgcc
     1021 gcggtagtga aagcggtcgg tcttgtggtg catgaccgtc cggaaggggc cggcgccgta
     1081 caggtcgctc caacccctga tggcctgatc cagatcgttg acgaggtagg cctcctgaaa
     1141 gaagcggtag ctgctcacgc ggtcggacat gtggtttccc cttccctcgg accctggctt
     1201 cctgcgacat tagcgcacgc cgggctgtta ggatagattt ggcgattgga tcaggccccg
     1261 ggggagggac catggccgga acaagcgtcg cagactcggc tctgcgccag tggctcgaca
     1321 ttcaatccat ccgcaacgtg ctggcgatcc acagccgcgg catcgatcgc gccgacggcg
     1381 atctgctgcg ttccgcctac catccggacg ccgaggtcgc ctacgggttc ttcgacggcc
     1441 cggccgccga gctctgcgag atcatgggca atcccagcga gcagatcacc atgcaccgca
     1501 ccagcaacat gtggatccgg ctgctggacg acgatcacgc catctccgag agctacgcgt
     1561 tcgtctactc ggaatcggcc gacgatgccg gctacgggca ggcgctcatc ggcggccgtt
     1621 atctcgaccg gcacgagaag cggggcgggg agtggcggat cagccaccgg acctacgtgc
     1681 tggaatggaa cgccaactgg ccgggcaccg gtaccgtgct gcccgactac gaaagcccca
     1741 tcaaccgccg cggcagcaag acgcccgacg atctcggcac ccgtctgctc aacgagtcac
     1801 agcaaattct cacttccgga gatgccacca tgaccattcc cgaggacctg gaagccgccg
     1861 ttgcggatgc gctcgcaaga caggacattc acgatctgat catggcccag gcgcgagcca
     1921 ccgaccgccg ggaccgggct ctgctcgagt cggttttcca cgaaggggcg acgatggatg
     1981 cgggcgggct gttcgacggc ccggcccccg agtattgcga tttcattctc gagatgacgg
     2041 cgcccctgaa gaagatgtac cactcggttg ccaacgagtg ggttcgggtg gaaggcgaca
     2101 gcggcgtggc ggagagctac gtgatcgcct tcacgacggc cgccgacgag gccggcggcg
     2161 acgtggacaa cctgtcgggt gggcgttatc tggaccgttt cgagcgccgc gacggggtct
     2221 ggaagttcac ccaccgtgcc tacgtgatgg actggaccat gcatcagccc accacggacc
     2281 agagcgacga tccggacagc atgtacgcga gcttaaccaa tcgcggcggt cactggccgg
     2341 acgacccggt ctacggcctg tgggggaagt gaaagatgag tgagacctct gacagacccg
     2401 ctcccggcga agcccgcatg ccggggccga gctaccggga cttcatcctg cgcgatcgcg
     2461 cggccccgcc cgctgccttt ctcgaggagt catacgagtt ccagggcgac gaggacattc
     2521 cttacagcaa ttacacctcc cacgatcatg cgcaggccga gttcgagaag atgtggccgc
     2581 gggtatggca gtgggcgtgc cgggaggagc acatccccaa tcccggcgac tttcacgtct
     2641 acgacgtcgg tgatctctcg gctatcgtgg tgcgcacgcc aagcggcgcc gtcaaagcct
     2701 atttcaacgc ctgcatgcat cgaggcactc agctgaagcc gccgggcatg accggatcgt
     2761 ccgaggatct gcgctgcccg ttccacggct ggacgtggtc gctggacggg gatctggtgg
     2821 acctgcccca ggcctgggac ttccctcacg tgaccgagga cagccatcgg ctgccggagg
     2881 tcaaggtcgg cctgtggggc gggttcgttt tcgtcaactt cgacccggac gcagcgccgc
     2941 tggaggacta tcttggcgtg ctgccgcggc acttcgccga cttccacatc gaggaccgct
     3001 acatcgagct gcacatccgc aagcgcctgc cggccaactg gaaggcgtcg gccgaggcgt
     3061 tcctggaggc ctaccacgtt cgcgagaccc acgccggcgg ccggccgaac acggaggtcg
     3121 ccacccagta cgacgtcttc ggcgacaacg tcacccggtt cattcacacc gtgggcgcgc
     3181 cgtgcccgct cgtcgatccg cccccgtccg agcagcagct gctggaaatg ctctgggtcc
     3241 gcggccggga agacgagccc gtgccccagg t
//
LOCUS       DBNHLM010000045         8782 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1323105, whole genome shotgun sequence.
ACCESSION   DBNHLM010000045 DBNHLM010000000
VERSION     DBNHLM010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8782)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8782)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8782
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1323105"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="AC16QF_02375"
     CDS             <1..840
                     /locus_tag="AC16QF_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6447429.1"
                     /translation="IDGFADRNRCEYVADFAAKLPGLILFRHILPVPVQDLAELFADI
                     DTYSFGPLEECAPAFGRVYAYLEAFLDTRQHQGPQGDLVDVLLAGVERDGAPCPWEDK
                     VHTALDVVFGGLATTTHAMSGAMYEMARRPEVRAAVMGDPERLGTAVEETVRLYAPVV
                     MVARSVRSDVNVGGVEIASGERVALNYAAASRDPEVCSNPREFDVNRHEVVHTAFGIG
                     PHRCIGEHLARLEIRVAVEEFLKRIPDVSLESDGAPTYESGQLRTMKELRLCWSAASA
                     SSF"
     gene            861..1049
                     /locus_tag="AC16QF_02380"
     CDS             861..1049
                     /locus_tag="AC16QF_02380"
                     /inference="COORDINATES: protein motif:HMM:NF028481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR6447430.1"
                     /translation="MAGIEFQTRSLGIAFAETAPKGRLPYIEHDGAQIAGSSISRPAH
                     TRRCAGQVEHRGVVRGRV"
     gene            complement(1219..2088)
                     /locus_tag="AC16QF_02385"
     CDS             complement(1219..2088)
                     /locus_tag="AC16QF_02385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6447431.1"
                     /translation="MSKRLGVSQTAIEIINGRWTMHIFGMRNDPATAELMSVLPNTSV
                     VGLWLTLFPLWVQFKLSGQLSFYPRIPKEGNETLADLVPSRTLYFDRLIRRALQNVEQ
                     FVLMGAGYDTRGYGMFADAGVTIFELDQPAVQSHKREMLQKTTINSNHVNFVTIDFSE
                     DDLFAKLADAGFDPSKKTLLLWEGVTLYLSLTEVQRTMRLVKRRAAPGSILLADLYAE
                     RMIELYGKSRVGAKLLELTDEGLDFGLSFAANWEQALNEFVEAQSMKVGEAHFLGSNN
                     EKGPYAVVAEMKC"
     gene            2297..2827
                     /locus_tag="AC16QF_02390"
     CDS             2297..2827
                     /locus_tag="AC16QF_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447432.1"
                     /translation="MDINWEAVGAIGEIVGAVAVVLTLGYLAVQIRQNTNASRAQSYQ
                     AVVDGHVGHHRTFTSDPKLIEIFGRGLQDIDTLDDLERGLFHSLVAPIVLQYQKYLYL
                     YREGMLPPDTFDMFERDVIATLITPGGRAWWLSSKGKWPEISNHLDQRIEDLQSEVVP
                     SHEDFGGHLSRIRRES"
     gene            complement(2912..3328)
                     /locus_tag="AC16QF_02395"
     CDS             complement(2912..3328)
                     /locus_tag="AC16QF_02395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6447433.1"
                     /translation="MIDYTSDLGRVDWEELRKDLIADDFHNGRTTAQLRLSFANSACV
                     AMAFDGQRCIGNGRLLSDGVGNAYVIDVWTQSDYRRQGVASRIMGMLIDGVPGQHIYL
                     QTDNAIGFYKSLGFREQPTGMGMIAGDYLRNSSRDE"
     gene            3706..3921
                     /locus_tag="AC16QF_02400"
     CDS             3706..3921
                     /locus_tag="AC16QF_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR6447434.1"
                     /translation="MEANMATGTVKWFNPTKGFGFIQPESGDKDVFVHISAVEKAGMA
                     ALTEGQRVSYELITEKGRSSAGNLQAT"
     gene            4309..5670
                     /locus_tag="AC16QF_02405"
     CDS             4309..5670
                     /locus_tag="AC16QF_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HIR6447435.1"
                     /translation="MSDYTKILLEEHEMPTRWYNIIPDLPEPPPPPLHPGTQQPATAE
                     DFAPLFPQALIAQEMSRERYIDIPEPVLEVYRLWRPSPLFRARRLERFLETPARIFYK
                     YEGVSPAGSHKPNTAVPQVWYNAQEGITKLTTETGAGQWGSSLAFACAQFGLECEVWQ
                     VAASYHAKPYRRTMMEIWGGKVHPSPSEVTEFGRSLLAENPDHPGSLGIAISEAVSQA
                     VADPQTRYALGSVLNHVLLHQTIIGEEALLQLAKVGETPDVLVGCTGGGSNFGGLSFP
                     FLREKLAGRINPTIRCVEPTACPTLTKGQYRYDFGDTAGLTPLMKMHTLGHSFIPEPI
                     HAGGLRYHGMAPLVSHLYELGMVEAIAIPQTECFEGALQFARTEGIVPAPEPTHAIAA
                     AIREALACRESGEEKVILTALCGHGHLDLASYDKYLHGEMVDLDLSDEVIEAAMKDVP
                     VLA"
     gene            5756..8170
                     /locus_tag="AC16QF_02410"
     CDS             5756..8170
                     /locus_tag="AC16QF_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6447436.1"
                     /translation="MNSTRSGLGRRLSVPAAFLGLACAFSGVAQSAHSAASSVIEEIV
                     VTARMREEGLQSAPIAISAFTEETLDYRGVERLDEIAKFVPSLTLENNPSFGGASNSA
                     AIYIRGIGQKEFLPTTEPGVGLYVDGVYVARSVGAILDIIDVERLEVLRGPQGTLFGR
                     NTIGGAISITTVKPEPGGEFEAMVSGALGTDDLVHLRGSAHLPLSDTVAARLSVASFQ
                     QDGYVDRADGIDLGEDDTLTGRLAIAWEPNERFRADLSFEATRDRENGPPMELIGIDF
                     TDLSQLQGVVLAPPPPMAFIHNVTAAAAGPGQPCAVTDVNGNGITYNPSIPNCYDSRY
                     IDPESGENQGTAPAFSKTDLFGVSANLRWDLSDTLTLKSITSWRDLDSEFARDGDHSP
                     QRISQFFDDLEQQQFTQEFQLLGAHERLNWILGAYYFTEDGDNVNILDFTVSNFRSGG
                     EFDNEAWAVFAQATFDITDRLHLTAGGRYTEEEKSFLPDQIIFQNYYAGISELVPEGN
                     PLAALDAPFLQAGERILPYLEKRIDIDEFTPMVNVSFDATDDVMLYATYSEGFKSGGF
                     TQRVFPPIVPGFTAPPGTPDIDLIPTYEPEFVKSYEIGAKATGWDGRLRLNGAVFRSD
                     YKDLQVQVFNSVAPVTQNIGKASINGLELEVTLVPADDWLIEGSLSWLDAEYDEIDTG
                     ITLIGEDNEFERVPEFTASAGISKEFQLDDAGSLVVRADWSYRSETFNDAYNTPQLET
                     DGYHLIDASVRWRNAGEDWTVALSGRNLTDEKYLVSGVYGTAFQSYEGMFDRGSQWRL
                     EVRKEF"
     gene            8204..8782
                     /locus_tag="AC16QF_02415"
     CDS             8204..8782
                     /locus_tag="AC16QF_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6447437.1"
                     /translation="MARVKLAKPESVDDPAVAGIFEWVTQMEGSVPNHFYVEMNFPEF
                     FTAKLGATKVLWEAGELTMPEIQHIGILVSKDNGCRYCTAAFCTILNHGLEAAEDYVA
                     ELVSVGTDAVSDLRLRTMLEYALQVNRDAAGVTDGDVARLRQIGLSDKGIVQLTHIVS
                     DFASYNRLNLALQTDYDYRDLWRQLAFGDGAG"
BASE COUNT         1802 a         2714 c         2574 g         1692 t
ORIGIN      
        1 atcgatggct ttgccgatcg taatcgatgc gagtacgtcg cggactttgc tgcgaagctt
       61 cccggattga tcctgttccg acacatcctg cccgtcccgg ttcaggacct tgcggaactg
      121 ttcgccgaca tcgacaccta ctcgttcggc ccgttggaag agtgtgcgcc ggcctttggc
      181 cgtgtctacg cctatctaga agcatttctc gacacgcgac agcaccaggg gccccaggga
      241 gatctggtcg acgtgctgct tgctggggtt gagcgtgacg gcgcgccatg tccgtgggag
      301 gacaaggtgc acacagcgct tgatgttgtg ttcggcggac tcgcaacgac cacccacgcg
      361 atgtcgggcg cgatgtatga gatggcccga cggccggagg tgcgggcggc cgtgatgggc
      421 gatccggaac gtttgggcac tgcggtagaa gagaccgtac gactttatgc ccccgtagtc
      481 atggtggcac gcagcgttcg ctccgacgtc aacgtagggg gtgtggaaat tgcgtcagga
      541 gagcgtgttg cactgaacta cgcggccgcg tcgcgggacc ccgaagtttg cagcaatccc
      601 cgcgagttcg acgtgaatcg tcacgaggtg gttcacactg cattcggaat cggcccgcat
      661 cgttgcattg gagaacacct ggctcggctc gaaattcggg tggcggtgga ggaattcctg
      721 aaacgtattc ccgacgtgtc gctggaatcc gatggagcac ccacttacga gagcggtcaa
      781 ctgcggacga tgaaagagct tcggctttgc tggtcggctg catcagccag ttcgttctga
      841 aagtcgagac ctgcctgcgc gtggcaggca tcgaattcca gaccaggagc ctcggaatcg
      901 ccttcgctga aacggctccc aagggcaggc ttccatacat cgagcacgac ggcgctcaga
      961 tcgccggttc cagcatctca cgacccgcac acacccgacg atgcgccgga caggtggagc
     1021 atcgaggtgt ggtgcgtggt cgtgtatgag gcttgcggcc tgctgcgcct gctgacaaag
     1081 atcctgctcg aacctgagcg ctccgcgctg gtgtcacctc gatagacgct tgaaggtatc
     1141 gccccctatc cccggagcct ggacgccgcc tgggaggtct cctacgcgtg atcagcagcc
     1201 atagttagag caatccgatc agcacttcat ttccgcgact accgcataag ggcctttttc
     1261 gttgttgctc cccaggaagt gagcttcgcc gaccttcatg gattgagcct ccacaaattc
     1321 gttcagcgct tgctcccagt tggcagcgaa ggaaagaccg aaatcgaggc cctcgtcggt
     1381 cagttccagc aacttcgctc caacgcgtga ctttccatac agctcaatca tccgctctgc
     1441 atacaggtcg gccaacagga tgctgccggg tgcagcccgt cgtttcacca accgcatggt
     1501 tcgttgaact tccgtgaggg acagatagag agtcacacct tcccagagaa gcagcgtctt
     1561 ctttgaagga tcaaacccag cgtccgctaa ctttgcgaac aggtcgtctt cgctgaaatc
     1621 gatggtgacg aaattgacgt gattgctgtt gatggtcgtc ttttgcagca tttcccgctt
     1681 gtgcgactga acagcgggct ggtcgagctc gaaaatagtc acgccagcat cggcgaacat
     1741 accgtaaccg cgggtgtcat agcctgcacc cattagcaca aactgctcca cattctgtaa
     1801 cgcacgtcta atgaggcgat caaaataaag ggtgcgtgaa ggcaccaggt ccgcgagcgt
     1861 ttcattcccc tccttcggaa ttcgcggata gaaggaaagc tgacccgaca gcttgaactg
     1921 cacccacagt gggaacaacg tcagccacaa ccctactact gacgtgttag gcaacaccga
     1981 catgagctcc gcagtggcgg gatcattgcg cattccaaat atgtgcatgg tccatcggcc
     2041 gtttatgatt tcaatcgcag tctgagaaac gcccagacgc ttgctcaccc ataactgtct
     2101 atagcctgtc agagcaatac caatcaccgc gagcggaaag taccggcatt gagtcggaag
     2161 ggaatgtttc ggttagtgat cgccaagttc tgctatcggg caggagcgga ctttttgata
     2221 tggtaagcgg catggccggt tacattttca gttcccaatt aagactaaaa cggatagccg
     2281 ttagcactcg cgaaagatgg atataaactg ggaagcagtt ggagcaatcg gagagatcgt
     2341 cggtgctgtc gcggttgttc taacccttgg gtatctggcg gttcagattc gtcagaatac
     2401 caatgcttcg cgggcacaga gctaccaagc cgtcgtcgac ggccacgtgg gccatcatcg
     2461 caccttcact tccgatccga agctgatcga gatctttggc agaggactgc aggacatcga
     2521 taccctggat gatctggagc ggggactttt tcattctctg gttgctccaa tcgtattgca
     2581 gtatcagaag tatctctatt tatatagaga gggcatgctg ccgcccgata cctttgacat
     2641 gttcgagcgt gatgtgatcg cgacgctcat cacgcccggc ggccgggcat ggtggctgag
     2701 ctcaaagggg aaatggccgg agatatcaaa ccacctggac caacgaatcg aagacctgca
     2761 aagtgaggtc gttccaagcc acgaagactt tggcggccat ttgtcgcgaa tcagacgcga
     2821 atcgtaatgg cgtcggtaga gaacgcggac ttcttggttg gtgcctgcta acttcctctt
     2881 tcggccatac gcggtcggcg gccattaccc atcattcgtc ccgagagcta ttgcgaaggt
     2941 aatctccggc aatcatgccc ataccggtag gttgttctcg aaagccgagc gacttgtaga
     3001 agccaatggc gttgtccgtc tgaaggtaga tgtgctgacc cgggacgccg tcaatcagca
     3061 ttcccatgat cctcgaggca acaccctgac ggcggtagtc gctttgtgtc cacacgtcga
     3121 tgacataagc gttcccaaca ccgtctgaca acagccgccc gttgccgata catcgctggc
     3181 cgtcgaatgc cattgcgaca caggcactgt tcgcaaacga caggcgtagc tgcgcagttg
     3241 tcctgccgtt gtgaaagtcg tcagcgatga ggtcttttcg caactcctcc cagtccaccc
     3301 ttcccagatc agatgtgtag tcgatcaagc caatctcctc ccgggtatcc tcaagcgctt
     3361 cgtatgttta cgttcgccga aaagcaatga ctccttttgg agtcggaaac agactaccgc
     3421 accgcagact ggaaacgtcc ctttaaggca ggaaaccgac gtcaggattc agccgcaaga
     3481 agtccgccgt cggccggaag caggccaact cccagcttgc ctgacacttg aagattgatg
     3541 cgcaacgcaa aggaacttct tgtgctgcag ccttaattcg cgtaccgttc gcacgcgatc
     3601 tacattttgg cctgccttct gcacggccca gacatgggct ttcgtttcaa gacacccact
     3661 atcgagattg ttcgtcaccg cgcccgattt gcgcgcggca ttgctatgga ggccaatatg
     3721 gctacaggaa ctgtaaagtg gtttaaccct accaaagggt ttggattcat tcagccggaa
     3781 tccggcgaca aagatgtgtt cgtgcacatc agcgcagtag agaaggcggg gatggcagca
     3841 ctgactgaag gtcagcgcgt tagctatgaa ctgatcaccg agaaaggtcg ctcctcagcg
     3901 ggcaacctgc aggctacctg atactgctgt ccttcagccc gtgcaggtca cctgcacggg
     3961 ctgctttcga gctctccccc tccagccagg cgcatcgggc gccacccgat cttcagtttc
     4021 tcgaagatcg ctagtaccac gcctgcccaa tgacaacgac tcagtaaagc ttcgctgacg
     4081 gacagggaga accggaagaa cgcgatcacg ccaggcaatt gcgatcgcag ctcttcttcg
     4141 cccaaccagc cattcagcgg cacgcgagcg tggtaacccc ccgatcgcgc tggtcaccac
     4201 cacttacggc tctggctgga gatctttcgt tttagccgaa cccgccctat gatcgggcgt
     4261 tttgcttgcc ggacctccag cttcgcaggc gcagacggac actgaagtat gagcgactac
     4321 accaagatcc tgctcgaaga gcacgagatg ccgacgcggt ggtacaacat cattcccgac
     4381 ctgccggaac cgcccccgcc gcccctgcat ccgggtaccc agcagccggc caccgcagaa
     4441 gacttcgcgc ccctgtttcc ccaggcgctg atagcccagg agatgagccg ggaacgctac
     4501 atcgacattc cggaacccgt tctggaggtc taccggctgt ggcggccaag cccgttgttc
     4561 cgagcacggc gcctggaaag gttcctggag acgcctgcca gaatattcta caaatatgag
     4621 ggcgtgagcc cggcgggttc ccacaagccg aacaccgccg ttcctcaggt ctggtacaac
     4681 gcccaggaag gcatcaccaa gctcaccacc gagaccggcg ccggccagtg gggcagctcg
     4741 ctcgcctttg cctgcgctca gttcggcctg gagtgcgagg tctggcaggt ggcggcttcc
     4801 tatcatgcca agccttaccg gcggacgatg atggaaatct ggggcggcaa ggtgcacccg
     4861 agcccctctg aagtcacgga gttcggccgt tccctgcttg ccgagaaccc cgaccatccc
     4921 ggatcgctgg ggatcgcaat ctctgaagcg gtgagccaag cggtcgccga tccgcagaca
     4981 cgttacgcgc tcggcagcgt gctcaatcac gtgctgctcc accagacgat catcggagag
     5041 gaagcactgc tgcagcttgc gaaagtcggc gaaaccccgg acgtgctggt gggttgcacc
     5101 ggcggcggat cgaatttcgg cgggctgtct tttccgttcc tgcgcgagaa actcgccggc
     5161 cgcatcaacc ctaccattcg ctgcgtggaa cccactgcct gcccgactct gaccaaaggt
     5221 cagtaccgct acgactttgg cgacaccgcg ggcctgacgc cgctgatgaa gatgcacacg
     5281 ctcggccact ctttcattcc ggagccgatc cacgcaggcg gcctgcgcta ccatggcatg
     5341 gcgccgctgg tctcgcatct ctacgagctc ggcatggtgg aggcgatcgc aattccacag
     5401 acggagtgtt tcgagggcgc gctgcagttc gcccgtaccg aaggcatcgt gccggccccc
     5461 gagccgactc atgccatcgc ggccgccatt cgcgaggcac tcgcctgccg ggaatccggt
     5521 gaggaaaagg tcatcctgac cgccttgtgt ggccacggcc acctggatct ggcgtcctat
     5581 gacaagtatt tgcacggaga aatggtcgat ctcgatctct ccgacgaagt catcgaggcg
     5641 gccatgaagg acgtgcccgt cctcgcatga gcgggcctag acgagcgaac acccgctttt
     5701 aaacgagcac caccgaacga acgacgacag aacaagcaat caggggagaa ccatcatgaa
     5761 ctctactcgg agcggcctgg gccgccgtct gtccgtgccg gccgcgtttc tcggcctggc
     5821 ctgtgctttt tcgggagtcg ctcagtcggc gcacagcgct gcgtccagcg tgatcgagga
     5881 aatcgtggtc actgcccgca tgcgtgaaga ggggctgcag agcgcgccca tcgccatttc
     5941 ggcattcacc gaggagacgc tcgattaccg gggggtggaa cgtctggacg agatcgcgaa
     6001 attcgtgccc agcctgacgc tggagaacaa cccgagtttc ggtggtgcct caaactctgc
     6061 cgccatctat atccgcggga tcggccagaa agagtttctg cccaccacgg aacctggcgt
     6121 gggtctgtac gtggacggcg tgtacgtggc tcgctcggtt ggcgcgatcc tggacatcat
     6181 cgacgtggaa cgcctggaag tcctgcgcgg cccccagggc accctgtttg gccggaacac
     6241 catcggcggc gccatatcca tcaccacggt gaaacccgag ccaggcggcg agtttgaagc
     6301 catggtatcc ggcgctctgg gcaccgacga tctggtgcat ctgcgcggct cggcccacct
     6361 gccgctgagc gatacggtgg ccgcccggct gtccgtcgca tccttccagc aggacgggta
     6421 cgtggaccgt gccgacggca tcgatctggg cgaggacgac acgttgaccg ggcgtttggc
     6481 aatcgcgtgg gagcccaacg aacgcttccg cgctgatctg agcttcgagg ccacacggga
     6541 ccgcgaaaac ggtccgccca tggagctgat cggcatcgac ttcaccgacc tcagccagct
     6601 gcagggtgtg gttctggcac cgcctccgcc catggcattc attcacaacg tcaccgccgc
     6661 cgcggccggg ccgggccagc cctgcgccgt caccgacgtc aacggcaacg gcatcaccta
     6721 caacccgagc atcccgaact gctacgacag ccgctacatc gatccggaaa gcggcgaaaa
     6781 tcagggcacc gcgcccgcgt tctccaagac ggacctcttc ggcgtgtccg caaaccttcg
     6841 ctgggacctc tccgatacgc tgacgctgaa gtccatcacg agctggcggg atctcgactc
     6901 cgagttcgcc cgggacgggg atcactcgcc ccagcgcatc tcgcagttct tcgacgatct
     6961 cgagcagcag cagttcaccc aggaattcca gctccttggt gctcacgaac ggctgaactg
     7021 gatcctgggc gcctactact tcaccgagga cggcgacaac gtgaacatcc tggatttcac
     7081 cgtctccaac ttccgctccg gcggcgagtt cgacaacgaa gcgtgggccg tcttcgccca
     7141 ggcgaccttc gacatcaccg accggctgca cctgaccgcg ggcggccgct acaccgagga
     7201 agagaagtcc ttcctgccgg atcagatcat cttccagaac tactatgcgg gcatcagcga
     7261 gctggtaccc gagggcaacc ccctggccgc gctggatgcg cccttcctcc aggcgggcga
     7321 gcggatcctg ccgtacctcg agaaacgcat cgacatcgac gagttcaccc ccatggtgaa
     7381 cgtctcgttc gatgcgaccg acgacgtcat gctctacgcc acttactccg aagggttcaa
     7441 gtcggggggc ttcacccagc gcgtcttccc gcccatcgtt cccggattta ccgcgccgcc
     7501 ggggacgccc gacatcgacc tgatccccac gtacgaacct gaattcgtga agtcctacga
     7561 gatcggcgcc aaagccacgg gttgggacgg ccggctgcgt ctgaacggcg ccgtgttccg
     7621 aagcgactac aaggatctgc aggtacaggt gttcaacagc gtcgcgcccg tcacccagaa
     7681 catcggcaag gcgtccatca atggcctgga gctggaggtg accctggtgc cggcggacga
     7741 ctggctgata gaaggcagcc tgtcgtggct ggacgccgag tacgacgaga tcgacaccgg
     7801 catcacgctg atcggcgagg acaacgagtt cgagcgggtg ccggagttca cggccagcgc
     7861 cggcatctcc aaggagttcc agctcgacga tgcgggctcg ctggtagtgc gcgccgactg
     7921 gagctaccgc tcggaaacct tcaacgatgc ctacaacacg ccgcagctgg agacggacgg
     7981 ctaccacctg atcgacgcca gcgtccgctg gcgcaacgcg ggcgaggatt ggaccgttgc
     8041 cctgtcaggc cgcaacctga ccgacgagaa gtatctcgtc agcggcgtct acggcacggc
     8101 cttccagtcc tacgaaggga tgttcgatcg tggcagccag tggcggctgg aagtccgcaa
     8161 ggagttctag cccgattcac aggggacgac gagaggccga aacatggcaa gagtgaaact
     8221 cgccaagcct gagtccgttg acgatccggc agtagccggc atcttcgagt gggtgaccca
     8281 gatggagggc agcgtgccca accatttcta cgtggagatg aatttcccgg agttcttcac
     8341 ggcgaagctc ggcgccacaa aggtgctctg ggaggccggt gagctgacca tgcccgagat
     8401 ccagcacatc ggcatcctgg tctcaaaaga caacggctgc cgctactgca cggcggcatt
     8461 ctgcaccatc ctcaaccacg gcctggaagc ggcggaggac tatgtggccg aactggtgag
     8521 cgtcggaacg gacgccgttt cggacctgcg gctgaggacg atgctggagt acgcactgca
     8581 ggttaatcgg gatgcggccg gcgtcaccga cggcgacgtc gcccgcctgc ggcaaatcgg
     8641 actcagcgac aagggcatcg tccagctgac gcacatcgtc agcgacttcg cttcctacaa
     8701 ccgtctgaac ctggcgttgc agaccgacta cgactatcgg gacctctggc gccagctggc
     8761 ttttggcgac ggcgccggtt ag
//
LOCUS       DBNHLM010000046         2185 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_724632, whole genome shotgun sequence.
ACCESSION   DBNHLM010000046 DBNHLM010000000
VERSION     DBNHLM010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_724632"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..976)
                     /gene="folD"
                     /locus_tag="AC16QF_02420"
     CDS             complement(125..976)
                     /gene="folD"
                     /locus_tag="AC16QF_02420"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010166832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIR6447438.1"
                     /translation="MTASILDGKSLAKSLRADLAAAVQERLESGGRPPGLAVILVGND
                     PASEVYVRHKSRDCEEVGFHSVVQHLSENVTQQDLEERIVALNANPSIDGILVQLPLP
                     AHIDSHRIIDRIDPLKDVDGFHPYNIGRLALRRPVLRPGTPKGIMQLLEVTGVPIRGL
                     DATILGASNHVGRPMGLELLLAGCTVTTAHKFSRDARASARSADILVSAVGKPGLVGG
                     DWIKPGAIVIDVGITRGEDGKLRGDVVFDEAMEVASWVTPVPGGVGPMTRVAMLQNTL
                     AAADGSD"
     gene            1149..1225
                     /locus_tag="AC16QF_02425"
     tRNA            1149..1225
                     /locus_tag="AC16QF_02425"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1183..1185,aa:Pro,seq:tgg)
     gene            1242..1318
                     /locus_tag="AC16QF_02430"
     tRNA            1242..1318
                     /locus_tag="AC16QF_02430"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1276..1278,aa:Arg,seq:tct)
     gene            1392..1467
                     /locus_tag="AC16QF_02435"
     tRNA            1392..1467
                     /locus_tag="AC16QF_02435"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1425..1427,aa:His,seq:gtg)
     gene            1517..>2185
                     /gene="tig"
                     /locus_tag="AC16QF_02440"
     CDS             1517..>2185
                     /gene="tig"
                     /locus_tag="AC16QF_02440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR6447439.1"
                     /translation="MHISVETMSGLERRLTISVPSQTFEGQITERLNRAAQQVRLPGF
                     RAGKVPMKEVRRRFGPAVRAEVAGELMQSSFVEAVRQEELAPAGSPNLEVVKMSPGID
                     FEFTATFEVFPKIELADLSGVHINRPNAQITELDVDEMVERLREQRKEWVAVDRGAED
                     GDRVTIDFAGRIDDEAFEGGSGEDVTFIVGAGQMIEDFDHGVRGMQAGTDGEFQATFP
                     DDYRA"
BASE COUNT          399 a          646 c          740 g          400 t
ORIGIN      
        1 gccaagaatg aaaatggcgt cggcgcgagg cgcctcgtgc cgcagcagag ccgcaagcgt
       61 tgcgaactgc ggctcgcttt catccgaaag atgaaggtcc gagatgtaga tccggtgcaa
      121 gggttcagtc cgatccgtcg gctgcggcga gggtgttctg aagcatggcc acccgcgtca
      181 tgggccccac gccgccgggc acgggcgtga cccagctggc gacttccatc gcctcgtcga
      241 acaccacgtc tccccgaagc ttgccgtcct caccccgggt aatgccgacg tcgatcacga
      301 tggcgccggg tttgatccag tcgcccccga cgagcccggg cttgcccacg gcagacacca
      361 ggatgtcggc cgaccgggct gaggcccggg cgtcgcggga gaatttgtgc gcggtcgtca
      421 ccgtgcatcc ggccagcagg agctccaggc ccatcggtcg gccgacgtga ttggaggcgc
      481 cgagaatggt tgcgtccagg ccgcgaatcg ggacaccggt gacctccagg agctgcatga
      541 tgccttttgg cgtgccaggt cgcagcacgg gtctgcgcag ggcgagacgg ccgatgttgt
      601 agggatgaaa gccgtccacg tccttgagag ggtcgatccg gtcgatgatc cggtgactgt
      661 cgatgtgggc gggaagcggt agctggacca gtatgccatc gatggacggg tttgcgttga
      721 gggcaacgat gcgttcctcg aggtcctgct gggtgacgtt ctcggacaga tgctgcacca
      781 cggagtggaa cccgacttcc tcgcagtccc gactcttgtg acgcacgtag acctccgagg
      841 ctggatcgtt tccgaccaga atgaccgcaa ggcccggtgg tctcccgccg gattcgagcc
      901 gctcctgcac cgccgccgcc aggtctgctc tgagcgattt cgccagactt ttgccgtcga
      961 ggatggaggc tgtcacgtca cccggattcc gcttgcctgt aggcccccat attacaacga
     1021 accggcctca gaccggccaa ccccgaccgc cgatcgaccc gttcagggcc cggcgtctgc
     1081 gtttgacggc tcagaacacc accagtatca tcgcgccgct tcgggcgcgg gttgtgcaac
     1141 agccacttcg gggtatagcg cagtctggta gcgcgcctgc tttgggagca ggatgtcggg
     1201 agttcaaatc tctctacccc gaccaatttc tcatcgagaa tgcgcccgta gctcaactgg
     1261 atagagcatc ggccttctaa gccgagggtt gaaggttcga gtccttccgg gcgtaccaga
     1321 ctcgtatcga gcggctcgat ggttcggtta gaatggcgcc gcccagcggt cgggtgaaag
     1381 accaggctac ggtggacgta gctcagttgg tagagctccg ggttgtgatc ccggcggtcg
     1441 tgggttcgag ccccatcgtc caccccattt caaccccagg gcggcagtta aaggtagcgg
     1501 caagcacggc agacagatgc atatttccgt tgagaccatg agcggcctcg agcgccggtt
     1561 gaccatctcg gttccgtcgc agacattcga aggtcagatc accgaaaggc tgaaccgagc
     1621 cgcacagcag gtgcgtctgc cgggtttcag agccggaaag gtgcccatga aggaggtgcg
     1681 ccgccgtttc gggccggcgg taagggcgga ggtggctggt gagctgatgc agtcgagctt
     1741 cgtggaagcc gtccgtcagg aggaactggc gccggccggg tcgcccaacc tcgaagtcgt
     1801 gaaaatgtcg ccaggtatcg acttcgagtt caccgctact ttcgaagtgt ttccgaagat
     1861 cgagctggcg gatttgagcg gcgtgcacat caacaggccc aacgcccaaa tcacggagct
     1921 cgacgtggac gagatggtgg agcgccttcg ggagcagcgt aaggagtggg tggccgtgga
     1981 tcgcggtgct gaggacggcg accgggtcac gatcgatttt gcggggcgca tcgacgacga
     2041 ggcattcgag ggcggctcgg gagaagacgt gaccttcatc gtgggcgcgg ggcagatgat
     2101 cgaggatttc gatcacggcg tgcgcggcat gcaggcggga accgatggtg agtttcaggc
     2161 aacgtttcct gacgactacc gggcc
//
LOCUS       DBNHLM010000047         8954 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1383295, whole genome shotgun sequence.
ACCESSION   DBNHLM010000047 DBNHLM010000000
VERSION     DBNHLM010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8954)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8954)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8954
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1383295"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="AC16QF_02445"
     CDS             complement(<1..740)
                     /locus_tag="AC16QF_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation SMC family protein"
                     /protein_id="HIR6447440.1"
                     /translation="MRLKQIKLAGFKSFVDPTTVILPSNRCAVVGPNGCGKSNIIDAV
                     RWVMGESSAKQLRGENLTDVIFNGSNSRKPTAIASIELIFSNEDGRIGGEYAAYAEIS
                     VRRQVTREAQSTYFLNGNKCRRRDIMDIFLGTGFGPRSYSIIEQGMISQLVEARPEDL
                     RVYLEEAAGISKYKERRRETENRIRHTRENLARINDIREELEKQLKHLDRQAKAAERY
                     RVLKEEERRLTAGLYTLRYDALEKELSAR"
     gene            complement(826..1509)
                     /locus_tag="AC16QF_02450"
     CDS             complement(826..1509)
                     /locus_tag="AC16QF_02450"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR6447441.1"
                     /translation="MATRANNNAEQQGCAPAISLTEQIAEHLAREIITGRLGANARIQ
                     ELRVADDLGVSRGSVREALLILESRHLIDLLPRRGAIVRDLTGEELVSFSELFTDLAA
                     AFFGKLARVPYLDLGLPAQAVEEMAMALGRNDLEGSIAARKAFVDGCLAELNDVYLVS
                     VLRALLPSALRLAHRAARHTGYEMRDTIRFHHALLEALSRGDDNRVGELVRAFGRREG
                     QLAAECRSS"
     gene            complement(1615..2808)
                     /gene="ccmI"
                     /locus_tag="AC16QF_02455"
     CDS             complement(1615..2808)
                     /gene="ccmI"
                     /locus_tag="AC16QF_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HIR6447442.1"
                     /translation="MSFWLMAGLMMLAGVLIVVVPLLRGGLQGGRTDRSVAVRSLYQE
                     RLAELDVDVAAGQLSPASRDAVAEELGSALLGDFEQTASGQGVDSRPVSPAVVLTLAV
                     LLPLVVLIVYLEVGDPGAQQIAGAEALLAMDPRTQRAEVAGWRDRLDSRVERRSDDAS
                     SWYLLGLANLQLGSFGRAAQAFAMSHALSGESPVVDAYWLQARYLSSGGEIDRETRVI
                     GDRLLKAQPNQPLVLEIYAIDAYRRGDYREAVALFSRALSGNLETEQRAVLAAGLQQA
                     RARLGDLVPSLDVSVTADGASPAGWTLFVIARPPGGGMPYAVVRRPSTSLPLDLRLDD
                     AVAMNADRPLSQAGEVELVVRLSRSGAPAAQAGDWEWRSQLLDLSRLTAPRRVEAVLR
                     EPDPS"
     gene            complement(2805..3269)
                     /locus_tag="AC16QF_02460"
     CDS             complement(2805..3269)
                     /locus_tag="AC16QF_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HIR6447443.1"
                     /translation="MRMADRRRAAIFGLLVAAGAALGAGPVDVHDFPDPATEARYRAL
                     IAEFRCPKCLNTNLAGSDAPIAKDLRRTVHRLVVVEGMSDAEVREFLQSRYGDFVLYD
                     PPFRFDTSILWLAPGLLLLVGLGVLYRVLRQPPPPKLSETDARRLREILEES"
     gene            complement(3256..3795)
                     /locus_tag="AC16QF_02465"
     CDS             complement(3256..3795)
                     /locus_tag="AC16QF_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HIR6447444.1"
                     /translation="MRRLRLFVPLLVFVIIVGFGYAGFKLNDPRELPSALLDRPFPEF
                     SASLLGGAGTVSRDALLGSPVLVNVWATWCPTCRAEHDELMRIRAETGLRLVGVNYKD
                     DPVKALRWLADFGDPYEFTLQDADGSLGVELGVYGAPESFLLDARGVIVYKRVGEVNE
                     RVWKEEILPALERLHDENG"
     gene            complement(3792..5747)
                     /locus_tag="AC16QF_02470"
     CDS             complement(3792..5747)
                     /locus_tag="AC16QF_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HIR6447445.1"
                     /translation="MIVELGHLALVIALCLSGLLAVFGLGGLNLAGGRLLRANLSLVT
                     GQAVFCALAFGVLTQAFLADDFSVAYVANNSNTLLPWYYKFSAVWGAHEGSFLLWTLI
                     MSGWTLAVAVLGRTLPLEFHSRVLGVMGFLNLGFLSFLIFTSNPFERLVPLSPAEGSD
                     LNPVLQDFGLIVHPPMLYMGYVGFSVAFAFAVAALLSGRLDAAWARWSRPWSNVAWAF
                     LTVGIALGSWWAYYELGWGGWWFWDPVENASFMPWLVGTALIHSLAVTEKRGTFKSWT
                     VLLAITTFSLSLLGAFIVRSGVLTSVHAFAVDPERGLFILGFLILIVGGSLLLYGVRA
                     GVVASRARFEPLSREAFILLNNVLLVVSMAVVLLGTLYPLAYEAVSGGDKISVGVPYF
                     NAGFVPLMLLLAAALATAPLLRWKRTERREILRGLLPALGVSLLLGVGAPLVLAGGVS
                     WQVALSVALACAILATHSRDLWTRARRGVARVPLAYWGMFAAHAGFAVAILGIALTSQ
                     WSVEKDLRMSPGEVVVLDGRAIEFLGVNPHRGSNYVADRGSFSVQEGDARIVLQPEKR
                     RYLAGGNVMTEAAIDAGFTRDLYISLGEPLQGGDWAVRIQVKPFVRWIWLGGLLMAFG
                     GCLAVFDSRYRRLKEKLLSAQVAEAAR"
     gene            complement(5744..6172)
                     /gene="ccmE"
                     /locus_tag="AC16QF_02475"
     CDS             complement(5744..6172)
                     /gene="ccmE"
                     /locus_tag="AC16QF_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017849438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HIR6447446.1"
                     /translation="MNPKRRNRLIVVSFLLLGTMATAFFVLVALNENLNLFYPPDQIV
                     AGEAPLGTQIRAGGMVLEGSVARTGNELDVEFVLTDYQGAAFTVLYSGILPDLFREGQ
                     GILVRGELDPSGVFRAEEVLAKHDENYMPPELDGMHAGPP"
     gene            complement(6169..6360)
                     /gene="ccmD"
                     /locus_tag="AC16QF_02480"
     CDS             complement(6169..6360)
                     /gene="ccmD"
                     /locus_tag="AC16QF_02480"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HIR6447447.1"
                     /translation="MSFETFGDFLTMGGHGPYVWLSYGLTLAVVLVNVLAPITGRRRY
                     LREQADAERRRQAAEGVRT"
     gene            complement(6357..7100)
                     /locus_tag="AC16QF_02485"
     CDS             complement(6357..7100)
                     /locus_tag="AC16QF_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HIR6447448.1"
                     /translation="MKEFWHRLGSPRWFYGISGPWLKILAGLAALLLGTGCIWGLAFA
                     PPDYQQGNSFRIIYVHVPTAIVAQTAYLVMGCAGVVLLVWRMKLADMVLATVAPFGMW
                     MTVLALMTGAIWGKPTWGAWWVWDARTTSMLVLLFLYFGIYALRQAIPRQEAAGRACA
                     VLAVIGVVNIPIIKYSVDWWLTLHQPATFKLTAAPSMPVEMWLPLAVNVVGIYCLFGA
                     NLLSRLRLEILQREAGTQWVRDMVLEEAR"
     gene            complement(7225..7896)
                     /gene="ccmB"
                     /locus_tag="AC16QF_02490"
     CDS             complement(7225..7896)
                     /gene="ccmB"
                     /locus_tag="AC16QF_02490"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005740276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HIR6447449.1"
                     /translation="MAGLFRAQLRRELQTTARSVGVAVNPLVFLFLAMTLFALGAGGG
                     PDDLREHAPGIIWVLVLLASMLSLEALFRSDYDDGTLEQMLLLAEPLFVPLLAKITAQ
                     WCVSGLAMTLLAPLAALILSLPAASVPTLAATLLLGTPALSLLGAVGAALTVGLRRGG
                     VLLGLLILPLYVPVLIFGAGAVSADMAGTGAAGQLYWLAAITMLALTTAPFGVQAALK
                     ISVEQ"
     gene            complement(7920..8483)
                     /gene="ccmA"
                     /locus_tag="AC16QF_02495"
     CDS             complement(7920..8483)
                     /gene="ccmA"
                     /locus_tag="AC16QF_02495"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009621121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HIR6447450.1"
                     /translation="MLRVVDAGCRRGGRQLFTELSLELGAGECVELHGPNGSGKSTLL
                     RCIAGLYPDFEGVVETAEFLYAGHRLGLNPLLSAEQNLAWYAALRGTADAVLPALERV
                     GMAGYERTACQHLSAGQQRRVALARLLVCRSPLWLLDEPLTALDGPGQELAVTLVTAH
                     MDGGGAVVAATHQPLGLERSRLLSLGR"
     gene            complement(8550..>8954)
                     /locus_tag="AC16QF_02500"
     CDS             complement(8550..>8954)
                     /locus_tag="AC16QF_02500"
                     /inference="COORDINATES: protein motif:HMM:NF023320.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleotide monophosphate
                     nucleosidase domain-containing protein"
                     /protein_id="HIR6447451.1"
                     /translation="DAEEVGTRIGRRVRRVRRHRRQVGDAYYFNWLLNVPLAHQQPFK
                     VTHENVAALRLSADLPAHELVVNLRRAFSAIVTGNVKDDGIRMIESHGPFELHSDRHL
                     IGALEQLLRSFSENGRMKLIGPYEPCYRVAAG"
BASE COUNT         1732 a         3097 c         2821 g         1304 t
ORIGIN      
        1 cgggcgctga gctccttctc gagggcgtcg tatcggagcg tgtacagccc cgcagtgagc
       61 cgccgctctt cttccttcag cacgcgatag cgttctgccg ccttcgcctg acggtccaga
      121 tgcttgagct gcttctcgag ctcttcgcgg atgtcgttga tccgggccag gttctcccgg
      181 gtgtgcctga tccggttctc ggtctcccgg cgacgctcct tgtacttgct gatcccggcc
      241 gcttcctcga gatacacccg gagatcctct ggtctggcct ccaccagctg gctgatcatg
      301 ccctgctcga tgatggagta gctgcgcgga ccgaatcccg tgccgaggaa gatgtccatg
      361 atgtcccggc gccggcattt gttgccgttg aggaagtagg tggactgggc ttcccgggtg
      421 acctggcgcc gaaccgagat ctcggcgtat gccgcgtact cgccgccgat ccggccgtcc
      481 tcgttgctga agatcagctc gatgctggcg atcgccgtgg gcttgcgcga attggaaccg
      541 ttgaagatga cgtcggtgag gttttcaccg cgcaactgct tcgccgaact ttcacccatg
      601 acccatcgaa ccgcgtcgat gatgttggac ttgccgcaac cgttagggcc aacgacggcg
      661 caccgattgc tggggaggat gacggttgtc gggtcgacga aagacttgaa gccagcgagc
      721 ttgatctgct ttagccgcat ggagaccgag agatcaccct gtcttaagac cgcggtagtc
      781 taaagcaatt gcctaccgag cgtaacaccc aaacgaccct gaaaatcagg aacttcggca
      841 ctccgctgcc agctgaccct cgcgccggcc gaatgcccga accaactccc ccacacgatt
      901 gtcgtcgccg cgtgacagcg cttcgaggag agcatggtga aagcggatcg tatcgcgcat
      961 ctcgtagccc gtgtgtcggg ccgcccgatg ggccagacgc agggccgatg gcaggagcgc
     1021 acgcagcacg gagaccaggt agacatcgtt gagctcggca agacagccgt cgacgaacgc
     1081 cttcctcgcc gcgatcgatc cttccagatc gttccggccg agggccatcg ccatttcctc
     1141 gaccgcctgg gccggcaggc cgagatcgag gtagggaacg cgggcaagct ttccgaaaaa
     1201 ggcagccgcc aggtccgtga acagctcgct gaagctgacc agctcctctc ccgtgagatc
     1261 cctgacgatg gcgccccggc ggggcaggag atcgatgagg tggcggcttt ccagtatcag
     1321 cagcgcttcc cggaccgacc ctctggacac tcccagatcg tccgccaccc gaagttcctg
     1381 tatccgggca ttggcgccaa gtcggcctgt gatgatctcg cgggccagat gctccgcgat
     1441 ctgttcggtg aggctgattg ccggagcaca gccctgttgc tccgcattgt tgttggctct
     1501 ggtcgccatg gcgatccttc gttgtacgga accggatgag agctatgacc gccccgcttt
     1561 cgattggttc gccgtcgccc aaaccggccc gagcaccccc tgccggccga agcgctagga
     1621 gggatccggt tcccgcagaa cggcttcgac cctgcgcggg gccgtcaatc gcgaaagatc
     1681 cagcaactgg gagcgccact cccagtcgcc cgcctgggcc gcgggagcgc ctgagcggct
     1741 cagccgcacc accagctcca cttccccggc ctgggagagc ggccggtcag cgttcatcgc
     1801 caccgcgtcg tccaaccgca ggtccagggg cagagatgtc gatggtctgc ggaccaccgc
     1861 gtacggcatc ccgcctcccg gcgggcgggc aatgacgaac agcgtccagc ccgcgggcga
     1921 ggcgccgtcc gcggtcacgg acacgtccag gctcggaacc aggtctccga ggcgggctcg
     1981 cgcctgctgc agcccggctg cgagcacggc gcgctgttcg gtctccaggt ttcccgagag
     2041 agcccgcgag aacagagcca ctgcctcccg atagtcgccg cgccgatagg cgtcgatggc
     2101 gtaaatctcg agcaccagcg gctggttggg ctgcgctttc agcagacgat cgccgatgac
     2161 cctggtttcc cggtcaattt cgcccccgga gctcaggtac cgagcctgga gccagtaggc
     2221 gtcgaccacc gggctttcgc ccgacagcgc gtgggacatg gcaaacgcct gcgccgcccg
     2281 cccgaagctg cccagctgaa gattcgcaag gcccaggaga taccagctgc tggcatcgtc
     2341 cgagcgccgc tcaacgcggc tatcgagccg gtctcgccaa ccggcgacct cggcgcgctg
     2401 ggtgcgcggg tccatggcga gaagcgcttc cgcgcccgct atctgctgcg cgcccggatc
     2461 tccgacctcg agatagacga tcagcaccac caggggcaac agcaccgcga gcgtcagcac
     2521 gacggcgggc gacaccggtc tggaatcgac gccctgcccg gacgccgtct gctcgaaatc
     2581 gccgagcaga gcagagccca gctcctcggc caccgcgtcg cgggatgctg gcgagagctg
     2641 accggccgcc acgtcgacgt ccagctcggc gagccgctcc tggtagaggg atcggaccgc
     2701 aacgctcctg tccgtccggc cgccctgaag ccctccgcgc agcagcggga caaccacgat
     2761 gagcacacct gcgagcatca tcagccccgc catcagccag aagctcatga ttcttccagg
     2821 atctcacgca gccgccgggc gtctgtctcg ctgagcttcg ggggcggcgg ctgccggagg
     2881 acgcgataca gaaccccaag gcctacgagc aacaggaggc cgggagccag ccagaggatc
     2941 gacgtgtcga accgaaaggg cgggtcgtaa agcacgaaat ccccgtaacg cgactggaga
     3001 aactcgcgaa cttcggcgtc gctcatgccc tcgacgacga cgagccgatg caccgtgcgc
     3061 cgcaggtcct tggcaatggg cgcatcggag ccggcgaggt tggtgttcag acacttcgga
     3121 cagcgaaact ccgcaatcag cgcacgatag cgcgcttccg tagccgggtc gggaaagtcg
     3181 tggacgtcca ccgggccggc ccccagggcc gcacccgcag caacgagcag gccgaaaatg
     3241 gccgcgcgac gacgatcagc cattctcatc gtgcaggcgc tccagcgcgg gcaggatttc
     3301 ttctttccag acgcgctcgt tcacctcccc gacccgcttg tagacgatca cccccctggc
     3361 atcgaggaga aagctttccg gagccccgta gacgccgagc tccaccccca gggatccgtc
     3421 cgcgtcctga agcgtaaatt cgtatgggtc cccgaaatcg gcgagccagc gcagcgcctt
     3481 gaccgggtcg tccttgtagt tcacccccac gaggcggagc cccgtctcgg cgcgaatgcg
     3541 catcagctcg tcgtgctcgg cccggcaggt gggacaccag gttgcccata cgttgaccag
     3601 caccggggag cccagcagag catcccggct tacggtgccc gctcccccga gaagcgaagc
     3661 cgagaactct ggaaacggac gatcgagcag cgccgagggg agctcgcgag gatcattgag
     3721 cttgaagccc gcatagccga aaccgacgat gatgacgaac accagcagcg gcacgaacag
     3781 gcgcagccgt ctcatcgcgc cgcctccgcg acctgagccg acaggagctt ctccttgagc
     3841 cgccggtagc gcgaatcgaa aaccgccagg cagccgccga aagccatcag aagcccgccc
     3901 agccagatcc agcgcacgaa cggcttcacc tgaatgcgaa cggcccaatc acctccttga
     3961 aggggttcgc ccagggagat gtaaaggtcg cgcgtgaacc cagcatcgat ggcggcctcg
     4021 gtcatgacgt tgccgcccgc caggtagcga cgcttctcgg gctgcagcac gatgcgggca
     4081 tcgccctcct gcacgctgaa gctgccccgg tcggccacgt agttggaacc acgatgggga
     4141 ttgacgccga gaaactcgat ggcccgacca tcgagcacga cgacctcgcc cggggacatg
     4201 cgcaggtcct tttccacgga ccactgcgag gtcagcgcga tgcccaggat ggccactgcg
     4261 aagccggcgt gcgcggcgaa catgccccaa taggccaacg gcacgcgggc gacgccgcgc
     4321 cgcgcccggg tccagaggtc tcgtgagtgc gtggccagga tcgcgcaggc caacgccacg
     4381 gaaagggcga cctgccagga tacccctccg gcaagcacca gcggcgcgcc cacaccgagc
     4441 agcaggctca ccccgagcgc gggcaacagc ccgcgcagaa tctcgcgtcg ctccgtgcgc
     4501 ttccagcgca gcaggggcgc ggtagccagc gccgctgcga gcagaagcat gagggggacg
     4561 aatcccgcat tgaaatacgg cacgccgacg gaaatcttgt ctccgcccga caccgcctcg
     4621 taggccagcg ggtagagcgt gcccagcaga accaccgcca tgctgaccac cagcagcacg
     4681 ttgttgagca gaatgaaggc ttcccggctg agcggctcga aacgggcccg ggacgcgacg
     4741 acgccggccc gcacgccgta gagcagcagg gagccgccca cgatgaggat gaggaagccc
     4801 agaatgaaca gccctctctc aggatccacc gcgaaggcgt gaaccgaggt gagaacaccg
     4861 gaccgcacga tgaacgcgcc cagcaggctc agcgaaaagg tagtgatggc caggagcacc
     4921 gtccagcttt tgaacgttcc gcgtttctcg gtcacggcaa gggagtgaat cagcgccgtg
     4981 cccaccagcc agggcatgaa ggatgcgttc tccactgggt cccagaacca ccagccaccc
     5041 cagccgagct cgtagtaggc ccaccagctg cccagcgcga tgcccacggt cagaaaagcc
     5101 catgccacgt tgctccaggg tcgcgaccaa cgcgcccagg cggcatccag ccgaccggac
     5161 agcagcgcgg ccacggcaaa ggcgaaggcc acggagaaac ccacgtagcc catgtagagc
     5221 atcggcgggt gaacgattag cccgaagtcc tggaggaccg gattcagatc agagccttcc
     5281 gcgggagaga gcggaacgag ccgctcgaag gggttgctcg tgaagatgag gaacgaaaga
     5341 aagcccagat tgaggaagcc catcaccccc agcacccggc tgtgaaactc cagcggcagg
     5401 gtccgtccca gcacggccac ggcaagcgtc cagccggaca tgatcagcgt ccagagaaga
     5461 aaagaccctt cgtgagcacc ccagacggcg ctgaacttgt agtaccaggg cagcagcgtg
     5521 ttggagttgt tcgccacgta ggcgacggaa aagtcgtcgg caagaaaagc ttgggtgagc
     5581 acgccgaagg ccaacgcgca gaacactgcc tggccggtga ccagcgacag gttcgcccgg
     5641 agcagcctgc cccccgccag attcagaccg ccgaggccga acacggcaag cagcccgctc
     5701 aggcagagcg cgatgaccag tgccagatgt cccagctcta cgatcacggc gggccggcgt
     5761 gcatgccgtc cagctccgga ggcatgtagt tctcgtcgtg cttcgccaac acctcctcgg
     5821 cccggaagac gccgctcggg tcgagctcgc cgcggacgag gattccctga ccttcgcgga
     5881 acagatcggg caggatgccg ctgtagagca ccgtgaaggc tgcgccctgg taatcggtga
     5941 gcacgaactc cacgtcgagc tcattgcccg tgcgcgccac gctgccctcg agaaccatgc
     6001 cacctgcccg gatctgcgtg cccagcggcg cttcgccggc cacgatctga tccggcgggt
     6061 agaagagatt cagattttcg ttgagcgcca ccagcacgaa gaacgccgtc gccatggtcc
     6121 cgaggagcag gaaggacaca acgatcaacc ggttacgccg tttcggattc acgtccgcac
     6181 accctccgcc gcctgccttc gtctttccgc atccgcctgc tcacgcaggt agcgccggcg
     6241 gccggtgatc ggcgccaaca cgttgaccag caccacggcc agcgtcagcc cgtagctcaa
     6301 ccacacgtac gggccgtgcc cacccatcgt cagaaagtca ccgaacgttt cgaagctcac
     6361 cgggcctcct cgagcaccat gtcgcgcacc cactgcgtgc ccgcttcccg ctgcaggatc
     6421 tccagtcgaa gacgactcaa caggttggca ccgaacaggc agtagatgcc gacgacgttc
     6481 accgccagag gcagccacat ctccaccggc atgctcggcg ccgcggtgag cttgaaggta
     6541 gcaggctgat gcagggtcaa ccaccagtcg acggagtact tgatgatggg gatgttcacc
     6601 accccgatga cggccagcac ggcgcaggcg cgcccggcgg cctcctgccg gggtatggcc
     6661 tgacgcagcg cgtagatccc gaagtacaaa aagagcagca ccagcatgga agtcgtgcgc
     6721 gcatcccaca cccaccaggc gccccaagtg ggcttgcccc agatcgcacc cgtcatcagc
     6781 gccagcaccg tcatccacat cccgaacggc gcaaccgttg ccagcaccat gtctgcaagc
     6841 ttcatgcgcc aaaccagcag gaccacgccg gcgcacccca tcacgagata agcggtctgg
     6901 gccacgatgg cggtgggcac gtggacgtaa atgatccgga aactgttgcc ctgctggtag
     6961 tcgggcgggg cgaaggcgag cccccagata catcccgtgc ccagcagcag cgccgccagc
     7021 cctgcgagga tcttcagcca gggaccgctg atgccgtaga accagcgcgg ggatcccagc
     7081 cggtgccaga attccttcag cgtcgccaag ggccgccctc acgtcgccgt cgtaacgttg
     7141 tcgtttgccg agcaatcata tgcatccgcg atatgcatcc gcgtagtttg acgcccagga
     7201 ggggccgggg ttcgcctttc atcgtcactg ctccacgctg atcttgagcg ctgcctgaac
     7261 gccgaagggc gccgtggtca gcgccagcat cgtgatggcg gcaagccagt agagctgacc
     7321 tgccgccccc gtccccgcca tgtccgcgct gaccgcgccg gcgccaaata ttaaaaccgg
     7381 cacatacaaa ggcaaaatca gaagccccag aagcaccccg cccctgcgca gcccgacggt
     7441 gagcgcagcg cccacggcgc ccaaaaggct cagggccggc gtgcccagca gcagggtcgc
     7501 cgcaagcgtc ggcacgctgg cggccggcag agacaggatc aacgccgcca gcggggcgag
     7561 cagggtcatg gccaggccgc tgacgcacca ctgcgcggta atcttcgcca gcaggggcac
     7621 gaagagcggc tcggccagca acagcatctg ctccagcgtc ccgtcatcgt aatcgctgcg
     7681 aaacaacgct tccagcgaca acatgctggc gagcagcacc agcacccaga tgatgcccgg
     7741 ggcgtgctcg cgcaggtcgt cgggcccgcc cccggcgcca agggcaaaga gcgtcatcgc
     7801 gaggaaaagg aacacgagcg ggttcaccgc cacacccacc gatcgtgccg tcgtctgcag
     7861 ctcgcgccgc agctgagccc ggaacaggcc ggccaccgtg ttctccttcg cggagccgct
     7921 catcgaccca ggctcagcag gcgcgaacgc tcgagcccga ggggctgatg ggtcgctgcc
     7981 accacggccc cgccgccgtc catatgcgcg gtgacgagcg tcacggcgag ctcctgcccc
     8041 gggccgtcca gcgcggtcag cggctcgtcg agcaaccaca gcggcgagcg gcagacgagc
     8101 agccgggcca gcgccacccg acgctgctgg ccggcggaca ggtgctggca ggcggtacgc
     8161 tcgtaaccgg ccatgcccac acgctccagc gccggcagca cggcgtctgc ggttccccgc
     8221 aaagcggcgt accaagccag gttctgctca gccgacagca gcgggttcag acccagccga
     8281 tgtcccgcat agagaaactc ggcagtctcg acgacgcctt caaagtctgg atacaggccg
     8341 gcaatgcagc gcagaagcgt cgactttccg ctgccgttgg ggccatgcag ctccacgcac
     8401 tcaccagccc cgagttccag ggagagctcc gtgaacagct gccgaccgcc tcggcggcag
     8461 ccggcgtcga cgacccggag caacggcgca gctgctgctc caacctggtc tggttccaaa
     8521 ggcgctggca aggcgatgac cgtccagacc tacccggcgg caacccggta gcagggctcg
     8581 taggggccga tgagcttcat gcggccgttc tcggagaagc ttcgcagcag ctgctcgagc
     8641 gcgccgatga ggtgccggtc gctgtgcagc tcgaacggcc cgtggctttc gatcatgcga
     8701 atgccgtcgt ccttcacgtt acccgtgacg atggccgaga aggcgcgccg cagattgacc
     8761 accagctcat gggccggcag gtcggcgctc aggcgcaggg cggcgacgtt ttcatgggtg
     8821 accttgaacg gttgctggtg agcgagggga acgttgagca accagttgaa atagtaggcg
     8881 tcacccacct ggcgccggtg ccggcgcacc cgacggaccc ggcggccgat ccgggtgccc
     8941 acctcctccg cgtc
//
LOCUS       DBNHLM010000048         6726 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_664620, whole genome shotgun sequence.
ACCESSION   DBNHLM010000048 DBNHLM010000000
VERSION     DBNHLM010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_664620"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /gene="folK"
                     /locus_tag="AC16QF_02505"
     CDS             <1..447
                     /gene="folK"
                     /locus_tag="AC16QF_02505"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR6447452.1"
                     /translation="AMGRLRGFADGDTRCSDLWRTSPVDCPPGSEDFINAAVSFRAAP
                     GLTPESLLADLKALEAEYGRTGQRLRNAPRELDLDLLLFDAETRDTADFVLPHPRAAD
                     RLFVLAPAAQVAPDVVWPEIGLTIRELLDELDSDERVVRLEAHGCL"
     gene            434..1300
                     /locus_tag="AC16QF_02510"
     CDS             434..1300
                     /locus_tag="AC16QF_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447453.1"
                     /translation="MVAFRRCLVIALWCLSAHALGYDGRIHQDLTFLAAKHFNRCTAG
                     TEVPPLTPLQIRYLAKTNVGLADRNAFARMFNWRYYDRAAEAEHSVMWAIDTRFHEHF
                     NEILARIDKADSMGKLYSEVGRIINYLQLVSSPPHTVPVFTSRFWRFSFSDRFDGFPV
                     DVEAVGQAIGEDCSFLAAEEEYAGVLRSIAADTIAAVRAPIEGMPATWEAFWEIADDP
                     EKFGDYGPAGNSFGRKTEFGCAQQRCVLLRADPLYAAFAAQRHAAAVKATLAAMLIVQ
                     RSQIATEVAGAQ"
     gene            1290..3665
                     /locus_tag="AC16QF_02515"
     CDS             1290..3665
                     /locus_tag="AC16QF_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HIR6447454.1"
                     /translation="MLSDLRVLDLSTDLGLMCGQLLADMGAEVQQWVLPDDAARLDTC
                     HWQAYTLGKGVQLIDWQADPEAVRRQVGCVDVIIESAPPGLMARLGLGYEELARINPE
                     VVHVSITGFGSTGPKADYEFSDLISAAAAGLVYVTGSEGQAPVRTTCPQAHGHASSDA
                     AVGVLIALFERARTGRGQHVDVSAQQSTTLALLNRALDGPVGQAKAERSAYGMTINGV
                     HVQNQFQARDGWVVCLPGVLPPIAAFMERLMAWVFEEGLCREEDLRWDWGGVALQIMQ
                     GQVTPEQWAPVTAGMEALIAGRTKAELMSEAISRRLLIAPVFSVADLLDSDHLAQRGF
                     LVEGGADLRLGPFARLSESPLPLTESGPRFWQERSAFDARPLAASPSGRDESGGDTEG
                     GGGPLAGLKVLDLFWVVAGPGATRMLADYGATVVHVESTRRQDMVRNVPPYIDGVPDP
                     ERAACHHSTNTNKLNLTLDISREEGRAVLADLIRWADVFTESFAPGAVARMGFDYATV
                     RTLNPNIIMISSCLMGQTGEWSGYAGFGNNGSAVSGFHALTGHPGQPPTGVFGPYTDF
                     TSVRFNALAILGAVMHGRRTGNGQYIDMSQGEAALHFLAPECLRYLRDGRVRGAEGNR
                     DEHFAPQGVYPTAGFDRWLALSVRSDAEWRALCALAGFDDLSNMSVEERAAAHDELDR
                     RLETWMETRPGEELEAELQSAGIAAHRTLDTHELGDDPQLVHRGHFIPVAHREFSNAV
                     VESSRLTLSASRAARPGTAPWFGIDNERVLSEILGYDGDRVRELASADVLS"
     gene            3683..4618
                     /locus_tag="AC16QF_02520"
     CDS             3683..4618
                     /locus_tag="AC16QF_02520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6447455.1"
                     /translation="MQMRLGITPWNLADRTASGLTEQARHAERLGYDSFWLPENHFNP
                     GAIPDPLMLLAAVAAGTQKIRLATTSYLLPLRNPLLAAEQVAVLDQLSGGRVTLGIGR
                     GYAPVMLRAFDVRPSEKRELFERSLALMRQAWRGEPVVLDDGETTVTLDPLPVQRPHP
                     PLWVAAFGPKALAQAGRLGLPYLASPVETLDTLEANYRRHGEAAAEAGHGPRDTIPVM
                     RTVFVSSNPRETRELLRRMNAEAEKSGRLAPGAEAHEWTIVGDDQFVRDRIVEYRERL
                     GVNHLIVTRLRLGGVDEGAVQASVGRVADIVGGLP"
     gene            complement(4661..5467)
                     /locus_tag="AC16QF_02525"
     CDS             complement(4661..5467)
                     /locus_tag="AC16QF_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014631.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160254 - tRNA queuosine(34) biosynthetic
                     process from salvaged queuosine or its precursors
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HIR6447456.1"
                     /translation="MQGGVRTAGGNEETSGNRLSEQDLNERRERVFIVLAGFFLCAMT
                     LLNVVGITRFIQLGPMALAVGVLPYPLTFLCTDLISELYGRKRANFLVWVGFTLNFFI
                     LGMLWLGSSIPAVDPATQPPWQVIELAEEIALPNGDVLSGSVELFQLLYACTSGAVFA
                     SMMAYIAAQFCDVQLFHFFKRLTKGRHLWLRNNFSTLTSQMVDSFMVISVTFGAAYFA
                     GSITLAAMLVLMGSNYLFKMTVALADTLPFYYLTGRLKRYLQIDNVHVLE"
     gene            complement(5467..6162)
                     /locus_tag="AC16QF_02530"
     CDS             complement(5467..6162)
                     /locus_tag="AC16QF_02530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6447457.1"
                     /translation="MKILLITGASAGIGLHTAQRFVAEGFAVVNLSRRRCPIDAVSQI
                     NCDLSVPGFIDNISNQLSGMLQEADRVVLVHNAARMGNDTAVETPSVALREALEVNVV
                     APNTLNYFTIPYMKPGSAIIYVGSTLAEKAVPGTYTYVVSKHALIGMMRSTCQDLAGQ
                     SIHTACVCPGFTDTEMLRQHVPAEAMASIRAMSAYGRLIDPDEIAETIFWAASNPVIN
                     GAVLHANLGQRES"
     gene            complement(6166..6678)
                     /locus_tag="AC16QF_02535"
     CDS             complement(6166..6678)
                     /locus_tag="AC16QF_02535"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl trahydropterin synthase family
                     protein"
                     /protein_id="HIR6447458.1"
                     /translation="MSRPSLTSIQISKEYLHFNSAHFTLFSDREREDLHGHTFYVAGA
                     FDAEVGPDGLAFDYNLVKTKLKQLCDELDEKTLLPERSPHLRLSEENGYLVAEFAGER
                     MPFLPRDVLTLPVRNVTVEELAPFLLERLGSDPEIRTLELQRIELRVSSGPGQWAAAT
                     WTAGQEIREV"
BASE COUNT         1105 a         2169 c         2285 g         1167 t
ORIGIN      
        1 gccatgggca ggcttcgtgg gtttgccgac ggcgacacgc gctgctccga tctgtggcgc
       61 acgtcgccgg tggattgccc accggggtcg gaagacttca tcaacgcggc ggtctccttt
      121 cgtgccgccc ccgggttgac gccggagagc ctgcttgctg atctcaaagc gctcgaggcc
      181 gaatatggcc ggaccgggca gcgcttgcgt aacgcccccc gggagctgga tctcgacctg
      241 ctgctgttcg acgccgagac gcgggacacg gccgacttcg tcctgccgca cccgcgcgcc
      301 gccgatcgcc tcttcgtgct cgcgccggcg gcgcaggtgg caccggacgt ggtctggccg
      361 gaaatcgggt tgacgatccg ggagctgctc gacgagctcg acagcgacga gcgcgtggtg
      421 cgtctggaag cgcatggttg cctttaggcg ttgcctggta atcgcgctct ggtgcctgtc
      481 ggcccatgcg ctcgggtacg atggccggat tcatcaggac ctgacctttc tggcggcaaa
      541 gcacttcaac cgctgcaccg cgggcaccga ggtcccgccg ctgacgccgc tgcagatccg
      601 ctacctcgcc aagacgaacg tgggcctcgc ggatcggaac gcgttcgccc gcatgttcaa
      661 ctggcgctac tacgaccggg ccgccgaggc ggagcactcg gtgatgtggg cgatcgacac
      721 gcgctttcac gagcatttca acgagattct tgcgcgcatc gacaaggccg acagcatggg
      781 gaagctgtac agcgaggtcg gccggatcat caactacctg cagctggtgt cgtccccgcc
      841 tcacaccgtg ccggtattca cctcgcggtt ctggcggttc agcttcagcg accgcttcga
      901 tggattcccg gtggacgtgg aagccgtggg gcaggccatc ggcgaggact gttccttcct
      961 cgccgccgag gaggaatacg cgggggttct ccgctccatc gccgcggata ccatcgcggc
     1021 ggtacgcgcg cccatcgaag gcatgccggc cacctgggag gccttctggg aaatcgccga
     1081 cgatccggag aagtttggtg actacggtcc ggccggcaac agctttggcc gcaagaccga
     1141 gttcggctgc gcgcagcagc gctgcgtgct cctgcgcgcc gacccgctct acgccgcgtt
     1201 tgccgcccaa cggcacgcgg cggcggtgaa agctaccctg gcagccatgc tcatcgtgca
     1261 gcgcagccag attgcaacgg aggttgccgg tgctcagtga tcttcgcgtg ctcgatctct
     1321 ccacggatct cgggttgatg tgcgggcagc tgctcgccga catgggggcg gaagttcagc
     1381 aatgggtcct gcccgacgat gcggcccgac tcgacacctg ccactggcag gcctacacgc
     1441 tcggcaaagg cgttcagctg atcgactggc aggccgatcc cgaggccgtt cgccggcagg
     1501 tcggctgcgt cgacgtgatc atcgagtccg ccccgcccgg gctcatggcg cgcctgggcc
     1561 tcggctacga ggagctcgcg cgcatcaacc cggaggtcgt tcatgtctcc atcaccgggt
     1621 tcggaagcac cggcccgaag gcggattacg agttctcgga tctgatttcc gcggctgcag
     1681 cggggctggt ctacgtgacg ggcagcgaag ggcaggcgcc cgttcgcacg acgtgtccgc
     1741 aggcccacgg gcacgcctcg agcgatgcgg cggtgggcgt gctcattgcg ctcttcgagc
     1801 gagcgcgaac gggccgcggt cagcacgtcg atgtgtctgc ccagcagtcg accacgctgg
     1861 cgcttctcaa tcgggcgctt gacggtcccg ttggccaggc caaggcggag cgcagcgctt
     1921 acggcatgac gatcaacggc gtgcacgttc agaaccagtt tcaggcccgt gacggctggg
     1981 tggtatgcct gccgggtgtg cttccaccca ttgccgcttt catggagcgg ctgatggcgt
     2041 gggtgttcga agaaggcctt tgccgcgagg aagacctcag gtgggactgg ggcggcgtgg
     2101 cccttcagat catgcagggg caagttaccc cggagcaatg ggccccggtg actgccggca
     2161 tggaagcgct gatcgctggg cgcaccaagg cagagctgat gtccgaggcc atcagccggc
     2221 ggctgctcat cgctccggtg ttctctgtag cggaccttct ggacagcgat cacctcgctc
     2281 agcgcggctt cctggtggag ggcggagcgg accttcggct cgggccgttt gcccgtttgt
     2341 cagagtcgcc gctgccgctg accgaaagcg ggccgcgatt ctggcaggaa aggtctgctt
     2401 tcgacgccag acccctggct gcctccccgt ccggccggga tgagtcgggc ggggacaccg
     2461 aagggggcgg cgggccgctg gccggattga aggtcctcga ccttttctgg gtggtggccg
     2521 gccccggcgc cacgcgcatg ctcgccgact acggggccac cgtggtgcac gtggagtcca
     2581 ctcggcggca ggacatggtc aggaacgtgc cgccctacat cgacggggtc ccggacccgg
     2641 agcgcgccgc ctgccatcac agcaccaaca ccaacaagct caacctgacg ctggacatca
     2701 gtcgggaaga gggtcgtgcg gttcttgccg acctgatccg ctgggccgac gtcttcaccg
     2761 aatccttcgc ccccggcgcg gtggcccgga tgggcttcga ctacgccacg gtgaggacgc
     2821 tcaacccgaa catcatcatg atctcgagct gcctgatggg ccagacgggg gagtggagcg
     2881 gctacgcggg gttcggcaac aacggctcgg cagtgtcggg ctttcacgcg ctcaccggcc
     2941 atccgggcca gccgcccacg ggcgtcttcg gcccctatac cgatttcacc tcagtgcgct
     3001 tcaacgccct ggccattctc ggcgccgtga tgcacggccg tcggaccggc aacggccagt
     3061 acatcgacat gtcccagggg gaggcggcgc tgcactttct cgcgcccgag tgcctgcgtt
     3121 acctgcgcga tggtcgggtc aggggtgccg agggcaaccg cgatgagcac ttcgcccccc
     3181 agggcgtcta cccgaccgcc gggttcgatc gctggctggc gctttcggtg cgcagcgacg
     3241 ccgagtggcg cgcgttgtgt gccctggcgg gattcgacga cctctcgaac atgtcggtcg
     3301 aggaacgggc ggccgctcac gacgagctcg accggcgcct ggaaacgtgg atggagaccc
     3361 gccccgggga ggagctggaa gcggagctgc aatctgccgg catcgccgcc caccggaccc
     3421 tggataccca cgagctcggc gacgatcccc agctggttca ccggggtcac ttcattcccg
     3481 ttgcgcaccg ggagttctcg aacgccgtgg tggaaagctc acggctgacc ctgtcggcaa
     3541 gccgcgccgc ccgacccggc acggcgccgt ggttcggcat cgacaacgag cgcgtgctaa
     3601 gcgagattct cggctacgac ggcgaccggg tccgcgagct ggcatctgcg gacgtgctgt
     3661 cgtgacggcg tggtgaggtg gcatgcagat gcggcttggc ataacgccct ggaatctcgc
     3721 tgatcgcacc gccagcgggc tgaccgagca ggcccggcat gccgagcggc tcgggtacga
     3781 ttcgttctgg cttccggaaa accacttcaa ccccggcgcc ataccggatc cgctcatgct
     3841 gctggccgcg gtggcggccg gcacgcagaa gatccgtctt gccaccacgt cgtacctgct
     3901 gccgctgcga aatccgctgc tggctgccga gcaggttgcc gtgctcgatc agctctccgg
     3961 cggtcgggtc acgctcggga tcggtcgggg gtacgcgccg gtcatgcttc gagcgttcga
     4021 cgtccggccc agcgagaagc gcgagctctt cgagcgctcc ttagccctca tgcggcaggc
     4081 gtggcgcggt gagccggtcg tgctggatga cggcgagacc accgtcaccc tggatccgct
     4141 gccggttcag cgcccgcacc cgccgctgtg ggtcgccgcc ttcgggccga aggcactcgc
     4201 ccaggcgggc cgtctcgggc tgccttacct cgcgtcgccc gtggagaccc tcgatacgct
     4261 cgaagccaat tatcggcgtc acggcgaggc ggcagcggag gccggccacg ggcctcgcga
     4321 tacgatcccg gtgatgcgca cggtattcgt ttcgtccaac ccgagagaga cacgggagct
     4381 gctccgccgg atgaatgcgg aagcggaaaa atccggtcgc ctggcgcccg gcgcggaagc
     4441 tcacgagtgg accatcgtcg gagacgacca attcgtcagg gaccggatcg tcgagtaccg
     4501 cgagcggctg ggggtcaatc acctgatcgt gacgcgactc aggctcggcg gggtggacga
     4561 aggtgccgtg caggcttccg tcgggcgggt ggcggacatc gtcggcggtc tgccctgact
     4621 gcctccaccg gatcacgccg gagcgatccg atcgggccgg tcactccagg acgtgaacgt
     4681 tgtcgatctg caggtagcgc ttgaggcgac ccgtgaggta gtagaagggc agggtgtccg
     4741 ccagcgccac cgtcatcttg aagaggtagt tgctgcccat cagaacgagc atggccgcca
     4801 gcgtgatgct gccggcgaag taggctgcgc cgaaagtgac gctgatgacc atgaaggagt
     4861 ccaccatctg gctcgtcagc gtggagaagt tgttgcgaag ccacaggtgt cgacccttgg
     4921 tcagccgctt gaagaagtga aaaagctgca cgtcgcagaa ctgcgcggcg atgtaggcca
     4981 tcatcgaagc gaacacggcg cccgaggtgc aggcatagag cagctgaaac agctccacgc
     5041 tgccgctgag gacgtcgccg ttgggcagcg caatctcctc ggccagctcg atgacctgcc
     5101 agggcggctg cgtggcggga tctacggccg gtatcgagct gccgagccag agcatgccga
     5161 gaatgaagaa gttcagggtg aagcccaccc agaccaggaa gttggcgcgt ttgcggccgt
     5221 acagctcgct gatgagatcc gtgcacagga aggtgagcgg gtagggcagc acgcccaccg
     5281 ccagggccat ggggcccagc tgtatgaaac gggtgatgcc gacgacgttt agcagcgtca
     5341 tggcgcagag gaaaaagccg gccagaacga tgaacacccg ctcgcgtcgc tcgttcaggt
     5401 cctgctcgct gaggcggttt cccgacgttt cctcgttgcc ccccgctgtt cttacgcctc
     5461 cctgcatcag ctttcccgct gacccagatt ggcgtgcaga acggccccgt tgataaccgg
     5521 gttgctggcg gcccagaaga tggtttcggc gatctcgtcc gggtcgatca gccgtccgta
     5581 ggccgacatg gcgcggatgc tcgccatggc ttcggccgga acgtgctgac gcagcatctc
     5641 ggtatccgtg aagcccgggc aaacgcacgc ggtatggatg ctctgccccg ccagatcctg
     5701 gcaggtcgag cgcatcattc cgatcagggc gtgcttgctc accacatagg tatacgtgcc
     5761 gggtaccgcc ttctccgcca gggtggagcc cacgtagatg atggccgagc ccggtttcat
     5821 gtacgggatg gtgaagtagt tgagcgtgtt cggtgcgacc acgttcacct ccagcgcctc
     5881 gcgcagggcc acgctcgggg tctccaccgc cgtgtcgttg cccatccggg cggcgttgtg
     5941 cacgagcacc acccggtcgg cctcctgcag cattccgctg agctgattgc tgatgttgtc
     6001 gatgaacccg ggaaccgaca ggtcgcagtt gatctggctg accgcgtcga tgggacagcg
     6061 gcgccgcgag aggttcacca cggcgaatcc ttcggcaacg aagcgttggg cagtgtgcag
     6121 cccgatcccc gcgctggctc cggtaatgag cagtatcttc atggcttaca cctcccgtat
     6181 ctcttgcccg gcggtccagg tcgccgcagc ccactggccg ggtcctgaag agacgcgcag
     6241 ctcaatcctc tgcagctcga gcgtgcggat ctcgggatcc gagccgagac gctccagcag
     6301 aaacggcgcc agttcctcta ccgtgacgtt gcgcacggga agggtgagga cgtcccgtgg
     6361 cagaaagggc atgcgctcgc cggcgaactc ggcgacgaga tagccgtttt cctcgctcag
     6421 gcgcaggtgg ggagaacgtt cgggcagaag ggttttctcg tccagctcgt cgcacagctg
     6481 tttcagcttg gtcttgacca ggttgtaatc gaacgccagc ccgtccggac ccacctcggc
     6541 atcgaaagca cccgccacgt aaaaggtgtg gccgtgcagg tcctcccgtt cgcgatcgga
     6601 gaacagggtg aagtgagcgg agttgaaatg aaggtattcc ttgctgatct gaatgctggt
     6661 gagagacggt cgcgacatcg ggcgatccta tcacagcgaa aacgtgcgaa cgggtgaatt
     6721 ccgcat
//
LOCUS       DBNHLM010000049        18115 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_724671, whole genome shotgun sequence.
ACCESSION   DBNHLM010000049 DBNHLM010000000
VERSION     DBNHLM010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 18115)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18115)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18115
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_724671"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1726)
                     /locus_tag="AC16QF_02540"
     CDS             complement(<1..1726)
                     /locus_tag="AC16QF_02540"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR6447459.1"
                     /translation="MYHLFPRPGKRLGVLAAAAVLLVTSAAASTASTPAVPAYVDDEL
                     LVAFEPGTPASEIAAAHRRNGAQIQQSIDSIGVQVLSIPSGSVPSLLQVYRNNPNVKY
                     AEPNYLRPLILPGEGSFGNNIDVISEQWSLHNQGQTLHTLVDPVTGVSPYPYTAPGAD
                     ISMDEAWVIEKGDPDVLVAVPDSGVDCQHGDLFMKCAHNEDYVTPTVDPYGTPIPELV
                     DRIGHGTHVAGTIGMDTDNDVGGAGIGWNVRVGSFKVCYQVEYVGIVIGSACQDADIA
                     AAIARAIELGYHVINMSFGQAAPSAVLKAALDDAVDAGLILVAAAGNNGNWQQFYPAA
                     YPEVISVGATTSWDDRAGFSTFSRDEDDWVDILAPGDSILATVPNVFCSSTATQCFGW
                     KRGTSMASPHAAGVAALVLSYLQRTDAANANRLEVRRRMQDCADKTGAMGQDMLVWSR
                     YGRLNAAAALTCGDTGGGGGGGGGGGGSDPTGSHIADLTSSSTSAGGNAWSSTVTVRV
                     HDASHGSLSGVTVNFVTDRSTDPTRSCTTGTDGSCTTEPVLQHKKNNSVTYTISSLSA
                     PDYTPGDNH"
     gene            complement(2003..2212)
                     /locus_tag="AC16QF_02545"
     CDS             complement(2003..2212)
                     /locus_tag="AC16QF_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447460.1"
                     /translation="MRTRVQIEDGDVSITFEPESEVERLCITELGDEISVSRSHKNIV
                     LRRRRDNVRKISDLEPVGQLAEESL"
     gene            2443..3792
                     /locus_tag="AC16QF_02550"
     CDS             2443..3792
                     /locus_tag="AC16QF_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR6447461.1"
                     /translation="MLYRAFTSRPGSLAALAAMMAALLAPSSHAADNGLVVYLSAYLS
                     GDYYYAPVPDLLPNESQQEHIQPIQLSLPRDFRRSVELGNHDISADGQIIVFAARTKR
                     RQDWDIYTGTPDYDQAEITNVQRIIHESGVREEDPRFDWTRDSSGLHRVVYKCDGDIC
                     IYDGAITKVVAEADCELWAPSFNSEGTAVSYVRRCDGRDSDRIWYAYLNAPYDREEVP
                     RPGNAGGPDRFSHFLDSDSLVYSHIMPDTGAAALWRYDGSSGSLTQLTFNTNTNSDDD
                     AYPDKSNPGRIAYIGWQEGTGYNLFIYENQQSYKLSNGVPMLAPAIFRPAGSTEPPPE
                     PPTEPPGTSAVHVGDLDVATGAGKRHWWSRVTVALHDGDENPAAGVTVALNTDSDSVS
                     CVTDEAGRCTTKRLNQNNSVASVEYTVVSVSAPEYNAAANHDPDGDSDGTSITAHQP"
     gene            complement(3897..5309)
                     /locus_tag="AC16QF_02555"
     CDS             complement(3897..5309)
                     /locus_tag="AC16QF_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HIR6447462.1"
                     /translation="MLYEPGRNIPATHHIDTTSFYSTASAARYAGAATVDVVGPDLQL
                     RKTVMAAGGTCGVDDATSIEVIYGDSVEYCFAMENAGDVPLTQVTLDDAKIELTIQPN
                     GGIMAAAETTVFKSDPVAMYVTDTNTAVANALEPLTGTKIGPEQSSATVNVLAADVSV
                     DKRIDTTTIVVCDAGDINPYCSEVNAQGLYDGVYSIVVSNAGPTDAWDVTVTDTLPDG
                     VKVISADSRCDISGAPLISCYLGDMKSATSETLYLSVEIDPGVFEFPWGTITNTACAN
                     TNPAQLDPDAKGKLKSSMITAEVPANINAYVDEHAISPIDIMTRGLLGANVSSWSDGT
                     PRSALGQARTKTSRQLIAAWCNEQVLGSKFAKHFLSWEKIRAIMVGEAYLSAGVVVGC
                     DGPCETRVRDVIDSITELNRVADEFNNSGLDLEDGLPQEPANTKAPKDDPTDPGVESP
                     ISTATSLDASTNKGKGNGRK"
     gene            complement(5445..7253)
                     /locus_tag="AC16QF_02560"
     CDS             complement(5445..7253)
                     /locus_tag="AC16QF_02560"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR6447463.1"
                     /translation="MSISTNHSLASNAALLKSKASKYAYYGVVIAVVAIVVATGLVSV
                     YNTGGISLDSLIEAQRENKALWLLDLMPFAFAFWGQYVSSMIAYEAGALVVDQTNELR
                     AQTTAIEQQAMHDLTHDKLTDLPNRALLRDRLEQAVSAAAHERSQLAILVLDLDRFKE
                     INDTLGHYNGDRLLKQVALRLEGAVKETDTVARLGGDEFAVLLHRISSPDDATDLGRK
                     IGKALQAPFGLEGLKLDVQASIGIAVFPDHGPDADTLLQRADVAMYIAKQQKSGCVSY
                     DPKLDKHSPRRLTLMGELRHAIDADELVLHYQPKIDIRSGTIVEAEALVRWQHPQYGM
                     LGPDEFIPLAERTGLIKPLTIWVLGRALRQQESWRGQGIDIGVAVNMSAHGLFDLELP
                     DLVAGLLAARGTAPDRLMLEITETSIMVDQQRALDILSRLADMGVRLSIDDFGTGYSS
                     LAYLSKLPVDEIKIDRSFVMDMIENAKHAMIVRATVDLAHNLDLEVVGEGTCEPEILE
                     HLRELGCDAAQGNYISPAVDVASFSQWLQNSQWKPRRTNPRQSTSETSGWPGRAKPRA
                     VAHTDLESIESAIQDLEEFVRQVDPKPDGSKAAGAS"
     gene            7591..9465
                     /locus_tag="AC16QF_02565"
     CDS             7591..9465
                     /locus_tag="AC16QF_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6447464.1"
                     /translation="MQQRIGKRDLILYAFMVAVLISIWLAMVQIDRQWQFIALTQEKL
                     DDQTRDIADLRRQFRRGISVSTETAGAGDPNALPDSWRGFKRAVAATEMPDYARGDWL
                     VYSFSSQVPSLTPYMSGDAYASSIQQWIIDGLVTRDPVTLEWLPHVAESWNISEDGLT
                     ISFVVRDGVVFSDGEPLTAEDVAFSWRFVMDEKIAAPRARAYLSRIDDVTVDGRTVTF
                     RFTEPYFGSFSLAGNLGILPRHFYSKYLESVAAAEEYNTSTGLLLGSGPYRLPSPTAW
                     KPGDLIELVRNERYWGWVPAPFDRRIWKTINSDAAQLTEFKNGGIDVYAALPLEYRDL
                     TQNEEIMKRTRSYEYYNPRGGYSYIAWNNLRDGKPTAFADRRVREAMTYLTDRERVID
                     EVLLGYAQPANGPFNPLGKQADPSLSVRGYDLDKAKALLAEAGYLDRDGDGVLESEAG
                     EPLAFKLTYPSGSDNYKRIMLLLKDLYVQAGVLMEPDPVDWPVLINTLNTKNFDAVSL
                     AWTSGFEIDIYQMLHSSQTEPGGDNFISYINPGLDELIETARQEMDEEARMAIWHQVH
                     REIWAEQPYTYLFRTKSLIFADRRIRNVQVVRAGLNEGGLWNMPMEWYVPTDAQKYSD
                     "
     gene            9499..10572
                     /locus_tag="AC16QF_02570"
     CDS             9499..10572
                     /locus_tag="AC16QF_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6447465.1"
                     /translation="MVNYVIRRLLLIFPTLLGITILVFAVMAAAPGGVGASLLSRSGD
                     LRPEERAAMERYINERYGLDKPMPMQYLRWLNNVSPIGFRKDENNEDAGFGFKVPDLG
                     MSFAKNRPVTQLVAQALPITLLLNALSVPLVFILSIVMGVYTARFRGQALDVGIGTAN
                     LALWSIPTIWMGVLLIGLLASEEYARIFPTSGLESLRADRWTFLPVVTEQGWQRGYLL
                     DVVWHLVLPIVCLTYTGFAVLTKLTRSAVLENLQADYARTARAKGASERTVLWYHVFR
                     NSLLPLLTVAASILPGMLVGSVIVESIFSIDGMGRLVVEAVQLRDREVVLSITLITSL
                     LTLLSYLIVDICYAAADPRVSFE"
     gene            10569..11756
                     /locus_tag="AC16QF_02575"
     CDS             10569..11756
                     /locus_tag="AC16QF_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6447466.1"
                     /translation="MTETAITRAVEKVGEESFSRRVTRDTLRNTGAKIGIAWVATVAV
                     LAVFAPFIANSHPYLMRVEGMWSSPLLAHLSWADVTMLVTFLAVVAAWRLRRRFGPGQ
                     LLVGVGVISLVTLLAAQQLVQVPRAVIYDQYRVGLAEGGIETAYFAPVPFSPTDRSRD
                     NPAPLHEPDSVHWFGTDEDGADVLSRMIHACRIALGIGLVATGIALALGIVYGGIMGF
                     FSGWLDIAMMRFLEIFESIPSLFLLLTIVAFFGRNIYLIMVVIGLTSWPGYARFLRAE
                     FLKLRNQDYVQAAEACGLPLASILFRHMLPNGVAPLLVAASFGIASAILAEATLSFLG
                     LGLIDEPSWGAMLNQAVGPTGTFALWLATYPGLAIFFTVFAYNLIGEALRDAIDPHLE
                     QRP"
     gene            11753..13378
                     /locus_tag="AC16QF_02580"
     CDS             11753..13378
                     /locus_tag="AC16QF_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6447467.1"
                     /translation="MSALLKVDHLVTRFESGGEATDAVRDVSFEIKPGETVCLVGESG
                     SGKSVTGLSILGLLPDSAVHPNGTITLSTSGRPEVSLLEAKEETLQKVRGARISMIFQ
                     EPMTCLNPVLTIGEQLVEPLELHLGLMGEAARNRAVDLLELVEMPEPERRLDEYPHRL
                     SGGQRQRVMIAMALACEPDLLIADEPTTALDVTIQAQILKLMADLQRKRNTALLFITH
                     DLSVVSEIADRVLVMQAGRIVESGTRDQVLFEPQHPYTKELLAALPANLERLAPPRER
                     TSEPLIRVQNLCVYFPVRKGVLQRHVDDFKAVDHVSLDINKGRVLAVVGESGSGKTTL
                     GRAILGLIKPTAGSVLFAGVDLAGLSRRALKPYRRELQIVFQDPQSSLNPRLSIASIL
                     TEPMAAHGIGGNQRERLERAADLMQKVDLDPMMLGRFPHEFSGGQRQRIGIARALALE
                     PKFIVCDEVTSALDVSVQARILKLLDQLKQELDLTYLFITHNIDVVRYFADDIAVMYQ
                     GTVVEQGLANEVCENPRHPYTKRLLAAVPRFER"
     gene            complement(13459..15459)
                     /locus_tag="AC16QF_02585"
     CDS             complement(13459..15459)
                     /locus_tag="AC16QF_02585"
                     /inference="COORDINATES: protein motif:HMM:NF048350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7507 domain-containing protein"
                     /protein_id="HIR6447468.1"
                     /translation="MQDIAGFGLNCTANDVQISKATVLEIIDECEYPGDKTTFVADFE
                     VVVTAKARYDIGLYFATDGDLNNDGAISQGPFPVNSGITSGCSVSTIGPENADFVDLD
                     DFPTTGDSCGDITKGDNPLHAPITITAVCNDPDGDGFLNLPNCTSWRQSGANEMCGTP
                     TDAFPGAPSKCRCDGDFQVDVPVPPAKLDVKKTAIPATINEPGAEVTFQVDVTNAGID
                     PNNGVTLNSIMDDIYGELMVPASGEIITNSCHSSMGATMAGGASSSCMFKAMVQGNAG
                     DKHVNTVYATGLDQRVPPNKIEGDPALATVTIVNVNPTLEVTKTASTDSVSENGEEVT
                     FTVTVKNTSVSSDPVTIGSLGDSVYGDLTAMGCADPGTIQPGDTYTCEFTVWLDAGVC
                     TSGAPCSETNTVTASGSDDDGTPVSDSASATVTITNDPSSIDVSKTASRASVPESGAN
                     VTFTFTVTNTSFSDTVTINSISDDVFGDLNGQGDCVTPGDLAPRGGSWSCSVTEWIGG
                     APDDEHTNVFTASGVDDDGENVQGNASATVNYDDVPPAATVALKVSALVTYDVEVRND
                     SAADELTIGSIVDDHYKDLTDVNNLMIQSSTCASGIVLAKKGEAGDTYQCSFTAEPGC
                     SPLANSATASVTDDDSAVAVTATSGTVSVSVSGLGACEAPTQ"
     gene            complement(15851..16339)
                     /locus_tag="AC16QF_02590"
     CDS             complement(15851..16339)
                     /locus_tag="AC16QF_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447469.1"
                     /translation="MGVSIVIGVLLAFAGAIFATKWAQEKIDFDLTGVRVALFALLMS
                     PFGVVAMGIFICTALAILGSGVILLQQAFAWLDTAVWNPHSIVDVLEGASYEAAKTWA
                     EDPQRWLGVHWIFSFLHPLVGGIMLVPIAVFTSSLMTLFCRAVQRLTKIGSAPHQAPA
                     VG"
     gene            16556..>18115
                     /locus_tag="AC16QF_02595"
     CDS             16556..>18115
                     /locus_tag="AC16QF_02595"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR6447470.1"
                     /translation="MDFATELLVRVLRASIWGTLLGGFAALIILAGITPAHAASPAFV
                     EGVVLVDYHPSASATDRAAARLAVGAVRATRLSPLAGRAEKIILPAGKGVERALKALR
                     RHPAVRVAEPDYVLTHNAAANDPLYLDGSLWGMYGNASTPSNTYGSQAAEAWGAGHTG
                     SAGVYIGVIDEGLQYTHEDLDANVWTNPGEIPDNNSDDDGNGFADDVHGWDFYNNDAS
                     VYDGTGDDHGTHVAGTIGGEGGNAVGVAGVTWDVTLISAKFLGPSGGSTSGAIAAVDY
                     ITMLKTTKGLNIVATSNSWGGGGFNQLLLDAINRGGDQGILFVAAAGNSSRDTDAGNY
                     YPSNYECTTASRSWDCVISVASITSSGTMSSFSNWGATTVDIGAPGSAIESTLPNNGY
                     GSYSGTSMATPHVSGAVALCSSIDTNMTAYDLRNAVLGTAAATASLAGKTTTGGRLDV
                     GTMVSVCTPPTSGVSGAPSSLSATATSTSSIALSWTDGVSGEDRWEVRRGAGNCTGME
                     TVGLAPPDSTTF"
BASE COUNT         3254 a         5942 c         5672 g         3247 t
ORIGIN      
        1 cgtgattgtc accgggggtg taatccggcg cgctcagcga gctgatggtg tacgtcacgc
       61 tgttgttctt cttgtgctgg agcaccggct cagtcgtgca ggacccgtct gtgcctgtgg
      121 tacacgaccg cgtggggtcg gtgctcctgt ccgtcacgaa gttcaccgtc accccggaca
      181 acgatccgtg gctggcatcg tgaacgcgca cggtgacggt ggatgaccag gcattgccgc
      241 ccgccgacgt gctggaggaa gtcaggtccg caatgtggct tcccgtcgga tccgagccac
      301 cgccgccacc accgccgcca ccgccgcctc cagtatcccc gcaggtgagc gcggccgccg
      361 cgttgagccg gccgtagcgg ctccagacga gcatgtcctg ccccatggcg ccggttttat
      421 ccgcgcagtc ctgcatgcgg cgccggacct ccaggcggtt ggcgttggca gcgtcggttc
      481 tctggagata gctcagcacc agagcggcaa ctccggccgc gtgaggcgac gccatggaag
      541 tcccgcgctt ccagccgaag cactgcgtgg ccgtggacga gcagaacacg ttgggaaccg
      601 tggcgaggat ggaatcaccg ggcgcaagga tgtcgaccca gtcatcttca tctctgctga
      661 acgtggaaaa cccggcccgg tcgtcccatg acgtggtcgc gcccacggag atcacttccg
      721 gataggcggc cggatagaac tgctgccagt tgccgttgtt gcccgcggca gccaccagga
      781 tgaggccagc gtccacggcg tcgtccagcg cggctttgag aaccgccgaa ggggcggcct
      841 gaccgaagct catgttgatg acgtgataac cgagctcaat tgcccgggcg atggcagcgg
      901 cgatatccgc gtcctgacag gcggacccga tcacgatgcc cacgtactct acctgatagc
      961 agaccttgaa cgagccgacg cgaacgttcc agccgatgcc tgcaccgccg acgtcgttgt
     1021 cggtatccat cccgatggtg cccgccacgt gggtgccgtg accgatgcgg tcaaccagct
     1081 ccggaatcgg cgtgccgtaa ggatcaacgg tcggcgtcac gtagtcttca ttgtgggcgc
     1141 acttcatgaa cagatcgcca tgctgacagt ccacgccgct gtccggcacg gcgacgagca
     1201 cgtcgggatc gcccttctcg atgacccatg cctcgtccat gctgatgtcg gcgccgggag
     1261 ccgtgtaggg gtaaggggac acacccgtta ccggatcgac gagcgtgtgc aacgtctggc
     1321 cctggttgtg caggctccat tgctcactga ttacgtcgat gttgttgccg aacgaacctt
     1381 cccccggaag tatcagcgga cgcagatagt ttggctcagc gtatttcacg ttcggattat
     1441 tgcggtagac ctggagcaag ctcggcacgc tgcccgacgg aatggataaa acctggacgc
     1501 cgatggagtc gatggattgc tgaatctgcg ccccgttacg gcgatgggca gcggcgatct
     1561 cggacgcggg cgttcccggt tcaaatgcca ccagcagctc atcatccaca taggccggta
     1621 ccgccggcgt cgacgcagtc gaagccgccg cgctcgtgac caggaggacg gccgctgcag
     1681 ccagaacacc gagtcgcttg cctggccttg gaaaaagatg gtacatcgta agtgtcgctc
     1741 cccagcatga gggcgggaac tacggggcaa acgcacccgt cgcgtctcgc cattgcaaag
     1801 taattcgatc acccgatgtt agaggtcggt gggtgctgag gaggtgaccg gtttctcagc
     1861 ggtttgtaaa cccggggcta caactcgcgt atccggtcga gcccgtgccc tggtgttgct
     1921 tctctgcaga agcgcggccg ccgggcggta acgggcagaa caacggggcg cttccatgca
     1981 ccgccttgtt ccgggttggc tgctaaagcg actcctcagc cagctgccct accggctcga
     2041 gatcgctgat cttgcggacg ttgtcgcgac ggcgccgcag cacgatgttc ttgtggctgc
     2101 gggagacgct gatctcgtca ccgagctcgg tgatgcacag gcgttccacc tcggactccg
     2161 gctcgaaggt gatgctgacg tcgccgtcct cgatctgcac tctggttctc atgtctttgt
     2221 cctctggggt tgtggatcgg acagatgcat tatccgaacg ctccccatgc cctggtatgg
     2281 gcgacatgtc agcggggttt gctggaattt ttggcgagaa cgcgttttga gacccgcgtc
     2341 atggaaaatt acggcgtaga gatagccctt gaattcgtca agataatttg cataatggcc
     2401 ggatggtagt tgatcggccg ggggggccga cggggggaca ccatgttgta ccgcgctttc
     2461 acatcccggc ctggttcact cgcggctctg gctgccatga tggctgcgct gctggcgccg
     2521 tcgtcacacg cggccgataa cggcctcgta gtctacctga gcgcttacct gtcgggtgac
     2581 tactactacg cgccggtacc cgatctcctg ccgaacgagt cccaacagga acacatccag
     2641 ccgatacagc tcagcctgcc ccgggatttc cgccgttccg tggagctcgg caatcacgac
     2701 atctccgccg acggccagat catcgtgttc gccgcgcgca cgaagcgaag acaggactgg
     2761 gacatctaca cgggcacgcc cgactacgac caggctgaaa tcaccaacgt tcagcggatc
     2821 atccacgagt ccggcgtgcg cgaggaggac ccgcgcttcg actggacgcg cgactccagc
     2881 ggcctgcacc gtgtcgttta caaatgcgat ggcgacatct gcatttacga cggtgccatc
     2941 accaaagtcg tggcggaagc cgattgcgaa ctctgggccc catctttcaa ctcggagggc
     3001 accgccgttt cctacgtgag gcgttgcgat gggcgggata gcgaccgcat ctggtacgcc
     3061 tacctgaacg ccccgtacga ccgggaggaa gtaccccgcc caggcaatgc gggcggcccg
     3121 gaccggtttt cccacttcct ggacagcgac agcctcgtct actcacacat catgcctgat
     3181 accggcgctg cggcactgtg gcgctatgac ggttccagcg gctccctgac gcagctcact
     3241 ttcaacacga acaccaactc cgacgacgac gcctatccgg acaagtccaa ccccggccgc
     3301 atcgcctaca tcggctggca ggagggcacg ggctacaacc tgttcatcta tgagaaccag
     3361 cagtcataca agctctcgaa cggcgtgccc atgctggcgc cggcgatttt ccgacccgcc
     3421 ggctccacgg aaccgccgcc cgaaccgccg acggagccac ctgggacctc tgcagtccac
     3481 gtgggcgatc tcgatgttgc caccggggcc ggcaagcggc actggtggtc gcgggtcacg
     3541 gtagcgctgc acgacgggga cgagaatccc gcggccggcg tcaccgtcgc gttgaatacg
     3601 gactccgaca gcgtcagctg cgtcaccgat gaggcgggga gatgcacgac caagcgtctg
     3661 aaccagaaca actcggtggc atccgtggaa tacacggtgg tttcggtttc cgccccggaa
     3721 tacaacgcgg cggcgaacca cgacccggac ggcgatagcg acggaacgag catcaccgcg
     3781 catcaaccct gaccggccag cccgggcggg gctcgctccc caccgacacc cgtcaacccg
     3841 gcaaaaaaaa gaccccgccg aagcggggtc tttggtttgg aggcagagcc ggtcgcttac
     3901 ttgcggccgt tgcccttgcc tttattggtt gaggcgtcga ggctcgtcgc ggtggagatc
     3961 ggcgactcga cacccggatc cgtcgggtca tcctttggcg ctttcgtgtt cgccggctcc
     4021 tgaggcaatc catcctccag gtcgagtccg gaattgttga actcgtcggc cacccggttc
     4081 agctcggtga tcgagtcgat cacgtccctg acccgggtct cgcaaggtcc atcgcaaccg
     4141 acgaccactc ccgcgctcag gtaggcttcg ccgaccatga tcgcgcggat cttctcccag
     4201 ctgaggaagt gcttggcaaa cttgctgccg agaacctgct cgttacacca ggctgcaatg
     4261 agctgacggg aggtcttggt ccgagcctga ccgagggcag accggggggt gccgtcgctc
     4321 caggaggaaa cgttggcgcc gagcaggccc cgcgtcatga tgtcgatggg agaaatggca
     4381 tgctcgtcca catatgcgtt gatgttcgcc gggacctccg ccgttatcat gctcgacttg
     4441 agcttgccct tcgcgtccgg atcgagctgc gccgggttcg tgttggcaca ggccgtattg
     4501 gtgatggttc cccaggggaa ctcaaacacg ccgggatcga tctccacgga caggtagagc
     4561 gtttccgaag tagccgactt catgtcaccc aggtagcagc tgatcagcgg ggcaccgctg
     4621 atatcgcagc gggagtcggc gctgatcacc ttgacgccgt cgggcagcgt gtcggtgacg
     4681 gtcacgtccc aggcgtccgt cgggccagcg ttgctgacaa cgatcgagta gacgccgtcg
     4741 tagagaccct gggcattgac ctcgctgcag tagggattga tgtcaccggc atcgcagacc
     4801 acgatcgtcg tcgtatctat ccgcttgtcg acagacacgt ccgctgccag cacgttgacg
     4861 gtggccgagc tctgctcggg gccgatcttg gtacccgtga gcggctctaa cgcgttcgca
     4921 accgcggtat tggtatccgt tacgtacatg gcaaccggat cggacttgaa gaccgtagtt
     4981 tctgccgcgg ccatgatgcc gccgttcggt tggatggtga gctcgatctt cgcgtcatcc
     5041 agcgtgacct gggtcagcgg gacgtcgcct gcattctcca tggcaaagca gtactccacg
     5101 gaatcgccgt agatgacctc gatgctggtg gcgtcgtcaa caccgcaggt gccgccggcc
     5161 gccatcaccg tcttgcgaag ctgcaggtcc gggccgacca catcgacggt ggctgctcca
     5221 gcgtagcgag cggcagaggc cgtgctgtaa aagctcgtgg tgtcgatgtg atgcgttgcc
     5281 gggatattgc gacctggttc gtacaacaac cggaaactgg cgcacaacgt gcaggggggt
     5341 agttaacagc tgcgccgaga accggcgcag aggcggacaa atcccttaca tcgcggaaaa
     5401 catatagaca aacggactat cggccaggcc gatgacggtg cgtgtcagct ggcgcctgcc
     5461 gccttggagc cgtcaggctt gggatcgacc tggcgcacga attcctccag gtcctggatg
     5521 gcggattcga tggattcgag gtccgtatgg gccaccgctc tgggcttggc acggcccggc
     5581 caaccggacg tctccgacgt cgattgacgg ggattggtcc ggcggggctt ccattgggag
     5641 ttctggagcc actgggagaa gctcgcgacg tccacggccg ggctgatgta gttgccttgg
     5701 gcagcatcac accccagctc gcgcagatgc tccagtatct ccggctcgca ggtgccctcg
     5761 cccaccacct cgaggtcaag attatgagca agatcgacgg tcgcccgcac gatcatggcg
     5821 tgcttggcat tctcgatcat gtccatcaca aaggatcggt cgatcttgat ctcgtccacg
     5881 gggagcttgc tgaggtacgc cagggaggag taaccggtgc cgaagtcgtc gatggacagg
     5941 cgcaccccca tgtcggccag acgcgagagg atgtccagag cccgttgctg gtccaccatg
     6001 atggaggttt cggtgatctc gagcatcagg cggtcgggcg cagtgccgcg agccgccagg
     6061 aggccggcca cgaggtccgg cagctcgagg tcgaacagcc cgtgggccga catgttgacg
     6121 gcgaccccga tgtcgatacc ctgcccgcgc caggattcct gctgccgcag cgctctgccc
     6181 agcacccaga tggtcaacgg cttgatcaga cccgtgcgct cggccagcgg tatgaactcg
     6241 tcgggcccca gcatgccgta ctgaggatgc tgccagcgca ccagcgcttc cgcctccacg
     6301 atcgtaccgg agcggatgtc gatcttcggc tggtagtgaa gcaccagctc gtccgcatcg
     6361 atcgcatgac gcagctcgcc catgagggta aggcgccggg ggctgtgctt gtccagcttg
     6421 ggatcgtagg acacgcaccc gctcttctgc tgcttggcga tgtacatggc cacgtcggcc
     6481 cgctgcagaa gggtgtcggc atccggtccg tgatcgggga aaacggcaat gccgatgctc
     6541 gcctgaacat ccagcttgag cccttcgagc ccgaacggcg cctgcagcgc cttgccgatc
     6601 ttgcggccca gatcggtggc atcatccgga gacgagatcc ggtgcagaag caccgcgaac
     6661 tcgtcaccgc cgagcctggc cacggtgtcc gtctctttca cggcgccctc gagacgaaga
     6721 gcgacctgct tgagcaggcg atcgccgttg tagtgcccga gcgtgtcgtt gatttccttg
     6781 aaccggtcca ggtccagaac caggatcgct agctgcgacc gctcgtgggc agccgcgctg
     6841 acggcctgct ccaggcgatc acgcagcaac gcccggttgg gcagatcggt gagcttgtcg
     6901 tgggtgaggt cgtgcatcgc ctgctgctcg atggcagtgg tctgagcgcg cagctcgttg
     6961 gtctgatcga ccaccaacgc gccagcctcg taggcgatca tggagctgac gtactggccc
     7021 cagaacgcga acgcaaaggg catgagatcg agaagccaaa gggccttgtt ttcccgctgg
     7081 gcctcgatga gcgaatcgag cgagatcccg ccggtgttgt agacggagac cagccccgtg
     7141 gcgaccacga tggcgaccac agcaatgacg acgccgtagt aggcgtactt gctcgctttg
     7201 ctcttcagca gtgccgcgtt gctcgcaagg ctgtggttgg tgcttatcga cacgccaact
     7261 cctacgtttg tggcggacgc cccactggaa cgcaaccgct aactttcccg gatgttagac
     7321 caaccggccg gtactcaagg tgacgacctt caaatatcca ggcaacgaat ctgctttgta
     7381 ggctcgtgac cgggcgccca agggctcggg caagtcgacc catgtccata ttgtatacaa
     7441 aattgcctat ttagccatcc gcgcccgggc ttgcgtaaaa acacagtcac cgggggctca
     7501 aaaggcctcg cgccggttgc ggcccgggac acgcgggtta gacttggcgc ttcgccgaca
     7561 ccggccccca ctcagcggaa atcgacccct ttgcagcagc gcatcggcaa acgcgacctg
     7621 atcctctatg ccttcatggt ggcggtgcta atcagcatct ggctggccat ggtccagatc
     7681 gaccgacagt ggcagttcat cgcgctcacc caggagaagc tcgacgatca gacccgcgac
     7741 atcgcagacc tcagacgcca gttcaggcgg ggcatttccg tctctacgga aacggccggt
     7801 gcaggagacc cgaatgccct gcccgactcc tggcggggtt tcaagcgggc cgtagcggcc
     7861 accgagatgc ccgactatgc ccggggcgac tggctcgtgt actcgttttc gagccaggtt
     7921 ccgagcctga caccctatat gtccggtgac gcctacgcgt cgagcattca gcagtggata
     7981 atcgacggcc tggtgacccg ggacccggtc accctggagt ggctgcccca cgtggccgaa
     8041 tcgtggaaca tcagcgagga cgggctgacg atcagcttcg tcgtccgcga cggcgtggtc
     8101 ttctccgacg gcgagcccct caccgcggag gacgtggctt tcagctggcg cttcgtcatg
     8161 gacgagaaga tcgccgcgcc gcgggctagg gcctacttat cccgcataga cgacgtcacc
     8221 gtcgacggcc gcacggtgac cttccgcttc acggagccct acttcggttc cttctctctg
     8281 gccgggaacc tgggcattct gcccaggcac ttctactcaa agtatctgga gagcgtggcc
     8341 gcagccgagg agtacaacac ctccaccggg ctgctgctcg gctcggggcc gtaccggctg
     8401 ccctcgccaa ccgcctggaa gccgggcgat ctcatcgagc tggtgcgcaa cgagcgctac
     8461 tggggctggg tacccgcgcc cttcgaccgg cgtatctgga agaccatcaa ctccgacgcg
     8521 gcccagctca ccgagttcaa gaacggcggc atcgacgttt acgccgcgct gcccctggaa
     8581 tatcgagatc tcacacagaa cgaagagatc atgaagcgaa ccaggtcgta cgagtactac
     8641 aacccgcgcg gcggttactc ctacatcgcc tggaacaacc tgcgcgacgg caaacccacc
     8701 gcgttcgccg atcgcagggt ccgagaagcc atgacctacc tgacggaccg ggagcgcgtc
     8761 atcgacgagg tcctgctcgg ctacgcccag cccgccaacg gcccgttcaa ccccctgggc
     8821 aagcaggccg acccgagcct gtccgttcgc ggctacgatc tggacaaggc gaaggccctg
     8881 ctcgccgaag ccggctacct ggaccgcgac ggcgatgggg tcctcgagtc cgaggccggc
     8941 gagccgctgg ccttcaagct cacctacccc tccggcagcg acaactacaa gcgcatcatg
     9001 ctgctgctca aagaccttta cgttcaggcc ggcgtgctga tggagcccga cccggtggac
     9061 tggcctgtac tgatcaacac gctgaacacc aagaactttg acgcggtgag cctcgcgtgg
     9121 acatccggat tcgagatcga catctaccag atgctgcatt cttcgcagac agagcccggc
     9181 ggggacaact tcatcagcta catcaacccg gggctggacg agctcatcga aaccgcccgc
     9241 caggagatgg acgaagaggc ccgcatggcc atctggcatc aggtgcaccg cgagatctgg
     9301 gccgagcagc cttacaccta tctgttccgg accaagtcgc tgatcttcgc ggatcggcgc
     9361 atccgcaacg tgcaggtggt tcgcgccggg ctcaacgagg gcggactgtg gaacatgccc
     9421 atggaatggt acgtgcccac cgacgcgcag aagtacagcg actgacggca ggctgagaag
     9481 gaagcagagc gcaacacgat ggtcaactac gtcatccggc gcctgctgct gatattcccg
     9541 accctgctgg gcatcaccat cctcgtcttc gccgtgatgg cggctgcccc cggtggcgtg
     9601 ggcgccagcc tgctctcccg ctccggagat ctgcgacccg aggagcgcgc cgccatggag
     9661 cgctacatca acgagcgtta cggcctggac aagccgatgc ccatgcagta cctgcgctgg
     9721 ctcaacaacg tctcgcccat cggcttccgc aaggacgaga acaacgagga cgccgggttc
     9781 ggcttcaagg tgcccgacct cggcatgagc ttcgccaaga accgtccggt gactcagctg
     9841 gtagcccagg cgctgcccat cacgctcctg ctgaacgcgc tttccgtgcc cctggtgttc
     9901 atcctgtcca tcgtgatggg cgtctacacg gcacgtttcc gggggcaagc cctcgacgtg
     9961 ggcatcggca ccgccaacct ggcgctgtgg tccattccca ccatctggat gggcgtgctg
    10021 ctcatcggcc tgctggccag cgaggagtac gcgcgcatct ttcccacatc cggcctcgag
    10081 agtctgcgcg ccgaccgctg gacgttcctg ccggtggtca ccgagcaggg ctggcagcgc
    10141 ggctacctgc tggacgttgt ctggcacctg gtgctgccga tcgtctgcct gacctacacc
    10201 ggcttcgccg tgctcaccaa gctgacccgc tcggcggtgc tggagaacct gcaggcagac
    10261 tacgcgcgca ccgcccgggc caagggcgcc tcggagagaa cggtgctctg gtatcacgtc
    10321 ttccgcaaca gcctgctgcc gctgctgacc gtagccgcct ccatcctgcc gggcatgctg
    10381 gtggggtccg tcatcgtgga gagcatcttc tccatagacg gcatgggccg tctcgtggtc
    10441 gaggccgtgc agctgcggga ccgggaagta gtgctgtcga tcaccctcat caccagcctg
    10501 ctcacgctgc tgtcctacct catcgtcgac atctgctacg ccgcggcgga tccgagggtc
    10561 agcttcgaat gaccgagacc gccatcacca gagctgttga gaaggtggga gaggaatcgt
    10621 tctcccgccg cgtgacccgg gacaccctgc gcaacacggg cgcgaagata gggatcgcct
    10681 gggtcgccac ggtggccgtt ctggcggtat tcgcgccgtt catcgccaac tcgcacccct
    10741 atctgatgcg cgtggaaggc atgtggtcca gccccctgct cgcccacctg agctgggcag
    10801 acgtcaccat gctcgtcacc ttcttggccg tcgtcgccgc ctggcgcctg cgccgccgat
    10861 tcgggcccgg ccagctgctc gtcggcgtcg gggtgatcag cctggtgacg ctgctggctg
    10921 cccagcagct cgtccaggtg ccccgtgcgg tgatctacga ccagtaccgg gtcggcctgg
    10981 ccgaaggcgg gatcgaaacc gcctacttcg cacccgtgcc gttctcgcca accgaccggt
    11041 cccgggacaa ccccgccccc ctgcacgaac cggacagcgt gcactggttc ggcaccgacg
    11101 aggacggcgc cgacgtgctc agccggatga ttcacgcctg ccgcatcgcc cttggcatcg
    11161 gcctggtggc caccggcatc gcgctcgccc tcggcatcgt ttacggcggc atcatgggct
    11221 tcttcagcgg ctggctggac atcgccatga tgcgctttct cgaaatattc gagtccatcc
    11281 cgtcactgtt cctgctgctt accatcgtgg cgttcttcgg ccgcaacatc tatctgatca
    11341 tggtggtcat cggcctgacg tcgtggcccg gctacgcccg cttcctgcgt gccgagttcc
    11401 tgaagctgcg caaccaggac tacgtgcagg cggcggaagc ctgcgggttg ccgctggctt
    11461 cgatcctgtt ccgccacatg ctgcccaacg gcgtggcgcc gctgctggtt gccgccagct
    11521 tcggcatcgc ctcggccatc ctggccgaag ccaccttgag ctttctcggt ctcgggctca
    11581 tcgacgagcc gagctggggc gccatgctca accaggccgt cgggcccacc ggcacctttg
    11641 cgctgtggct cgccacctat cccgggctcg ccatcttctt caccgtgttc gcctacaacc
    11701 tgatcggcga agccctgcgc gacgccatcg acccccatct cgagcagcgg ccgtgagcgc
    11761 gctgctcaag gtcgaccatc tggtcacgcg cttcgagtcg gggggcgagg ccaccgacgc
    11821 ggttcgcgac gtctcgttcg agatcaagcc gggggagacg gtctgcctgg tgggggaatc
    11881 cggctccggc aagagcgtca ccggcctctc catcctgggc ctgctgccgg attccgccgt
    11941 gcacccgaac ggcaccatca cgctcagcac cagcggcagg cccgaggtct cgctgctgga
    12001 ggccaaggag gagacgttgc agaaggtgcg cggcgcgcgc atctccatga tctttcagga
    12061 gcccatgacg tgcctgaacc cggtgctcac catcggcgag cagctggtgg aacccctgga
    12121 gctgcatctc ggcctcatgg gcgaggcggc ccgcaaccgg gccgtggatc tgctggagct
    12181 ggtggaaatg cccgagcccg agcggcgcct ggacgagtac ccgcaccggc tctccggcgg
    12241 tcagcgccag cgcgtgatga ttgccatggc gctggcctgc gagccggatc tgctcatcgc
    12301 cgacgagccc accacggctc tggacgtgac catccaggcc cagatcctga agctcatggc
    12361 cgacctgcag cgcaaacgca acaccgcgct gctcttcatc acccacgatc tcagcgtggt
    12421 ctcggagatc gccgaccgcg tgctggtcat gcaggcgggc cgcatcgtgg agtcaggcac
    12481 tcgggatcag gtgcttttcg agccgcaaca tccctacacc aaggagctgc tggcggccct
    12541 gcccgcaaac ctcgagcggc tcgcaccgcc ccgggaacgc acgtccgagc cgctcatccg
    12601 ggtgcagaac ctctgcgtgt acttcccggt ccgtaaaggc gtgctgcagc gccacgtcga
    12661 cgacttcaag gccgtggacc acgtcagcct ggacatcaac aaaggccggg tgctcgccgt
    12721 ggtgggcgag tcgggctcgg gcaagaccac cctgggcaga gccatcctcg ggctcatcaa
    12781 gcccacagcg gggtccgtac tcttcgccgg cgtcgatctg gccggtctct cgcgccgggc
    12841 gctcaagccc taccgccggg agctgcagat cgtcttccag gacccacagt cgtcgctgaa
    12901 cccgcgcctg tccatcgcct ccatcctcac cgagcccatg gccgcccacg gcataggggg
    12961 caaccagagg gagcggctgg aacgcgcagc agatctcatg cagaaggtgg acctggatcc
    13021 catgatgctc ggccgcttcc cccacgagtt ctccggcggc cagcgccagc ggatcggcat
    13081 tgcgcgggcg ctggcgctgg aaccgaagtt catcgtctgc gacgaggtca ccagcgcgct
    13141 ggacgtcagc gtccaggcgc gcatcctgaa gctgctggac cagctcaagc aggagctgga
    13201 cctcacctat ctgttcatca cccacaacat cgacgtggtg cgctacttcg ccgacgacat
    13261 cgccgtgatg taccagggca ccgtggtgga gcagggcctt gcgaacgagg tctgtgaaaa
    13321 tccccggcat ccctacacca agcggctgct ggcggccgtt cccagattcg agcgatagtt
    13381 cctacaccgg gccaaaaaaa agggagcgga ttctcatccg ctcccaaaag caaagggagg
    13441 tgctgtcccc tttggtggtt actgggtcgg ggcctcgcag gcgcccaggc cggagaccga
    13501 caccgagacc gtgccgctgg tggcggtaac cgccacggcg ctgtcgtcgt cggtgacgct
    13561 tgccgtcgcc gagttcgcca gcggcgagca gcccggctcg gccgtgaacg agcactggta
    13621 ggtgtcgccg gcttcgccct tcttcgctag cacgatgccg gaggcgcaag tgctgctctg
    13681 gatcatcagg ttgttcacgt ccgtcagatc cttgtagtga tcgtccacga tggagccgat
    13741 ggtcagctca tctgccgcgg agtcgtttcg gacctcgacg tcgtaggtga cgagggcgga
    13801 gactttcaag gcaaccgtcg ctgccggcgg gacgtcgtcg tagttgaccg tcgccgaggc
    13861 gttgccctga acgttttcgc cgtcgtcatc aacaccgctg gcggtgaaga cgttggtgtg
    13921 ctcgtcgtcg ggcgctccac cgatccactc agtcacggag caggaccagg atccgccgcg
    13981 cggcgcgagg tcaccggggg tcacgcagtc gccttggcca ttgaggtcgc cgaagacgtc
    14041 gtcggagatc gagttgatcg tcaccgtgtc cgagaaggac gtgttggtca ccgtgaacgt
    14101 gaaagtcaca ttggcgccgc tctccggaac cgatgcgcgg cttgccgtct tgctcacgtc
    14161 gatggacgac gggtcgttgg tgatcgtgac cgtggccgac gcgctgtccg agaccggcgt
    14221 gccgtcatcg tcgctgccgg aggcggtcac cgtgttcgtc tcggagcagg gcgcgccgct
    14281 ggtgcagacg cccgcgtcca gccagacggt gaactcgcag gtgtaggtat cgcccggctg
    14341 gatcgtgccg ggatcggcgc aacccatggc ggtcagatcg ccgtaaacgc tgtccccaag
    14401 cgatccgatg gtcaccgggt cgctcgaaac gctggtgttc ttcaccgtta cggtgaacgt
    14461 gacttcctcg ccattctcgc tgacgctgtc cgtggacgcc gtcttcgtca cctcgagggt
    14521 cggattgacg ttgacaatgg tcaccgtcgc cagggcggga tccccttcga tcttattcgg
    14581 cgggacacgc tgatccagac cggtcgcgta aaccgtgttg acgtgcttgt cgcccgcgtt
    14641 tccctgcacc atggccttga acatgcagct gctggaggct ccgccagcca tggtcgcgcc
    14701 catgctgcta tggcagctgt tcgtgatgat ctcgccgctt gcgggaacca tgagttcccc
    14761 atagatgtcg tccatgatgg agttgagggt tacgccgttg ttagggtcga tacccgcgtt
    14821 ggtgacatca acctggaaag tcacctcagc cccaggctcg ttgatcgtcg ccgggattgc
    14881 cgtcttcttc acgtcaagct tggctggcgg aacgggcacg tcaacctgga agtcgccgtc
    14941 gcagcggcac ttcgacggtg cgcccgggaa ggcgtccgtg ggggttccgc acatctcgtt
    15001 cgccccagac tgccgccagc tggtgcagtt gggcaggttc aggaagccat cgccgtccgg
    15061 gtcgttacac acggccgtaa tggtgatggg cgcgtgcagc ggattgtcac ctttcgtgat
    15121 gtcgccgcag gaatcccccg tggtcgggaa atcgtccaga tcgacgaagt cagcgttctc
    15181 cggtccgatg gtggataccg agcacccgct tgtgatgccc gagttaaccg gaaacggacc
    15241 ctgagaaatc gcaccatcgt tgttcagatc gccgtcggta gcgaaataaa gcccgatgtc
    15301 gtagcgcgcc ttggcagtga ccaccacctc gaagtcggcg acgaacgtcg tcttgtcccc
    15361 cggatactca cactcatcaa tgatctcgag caccgtggcc ttcgagatct gcacgtcgtt
    15421 ggccgtacag ttgagcccga agccggcgat gtcctgcata catccggctt ggaccgtttc
    15481 cgccgccgcg ggcgatgcgt gtccgagcag gacggcggtg aggaatgcag cgcccaccgc
    15541 gaacggtcca cgccacgaag atgaaaaaaa cctcatgaaa gtaacccctg tgtaacctgt
    15601 cctctacgct acgaaggggg ttccgctaaa gctgtggcag ctatgtggct gggtgtcagc
    15661 aggatgtctt ggcctgcagc ggggatatgg ggaggtgtga gggaaatgtc ttggacgtaa
    15721 gctcgatcag agcatatccg attgcaattc ctacatgccg ttgcttaaca agtcaccgga
    15781 cacagatcgt aatctaagcc ttcgcggcga gacccgcaag ctgaaccggg tcaagaaaac
    15841 cgcagcgggt ttaaccgacg gccggcgcct gatgcggggc gctgccgatc ttggtcaacc
    15901 tttgaacggc ccggcagaag agcgtcatca gagaggatgt gaagacggcg atgggcacca
    15961 gcatgatgcc gccgacgagc gggtgaagga agctgaagat ccagtgcacg cccagccacc
    16021 gctgcggatc ctccgcccag gtcttggccg cctcgtagct ggctccctcc agcacgtcca
    16081 cgatggagtg ggggttccac acggcagtat ccagccaagc gaaagcctgc tgcaaaagga
    16141 ttactccgga accgagaatc gccagggccg tgcagatgaa gatgcccatg gcgacgacgc
    16201 cgaacgggga catcaggaga gcaaaaagcg caacgcggac gcccgtcagg tcgaagtcga
    16261 tcttctcctg cgcccacttg gtcgcgaata tcgctcccgc aaaagccagc agaacgccaa
    16321 tgactatgga tacacccact acgcccggct ccttccgaga cactcagcca aacgagaggg
    16381 tatcggagga cgacgatggg gtacgtgacc ggcctcacaa cggaggtgtt acgagctctc
    16441 ggaaggtcca gctggcaccc cgatcccttc ctggcaatgc caatgccaca gcactagcaa
    16501 ttttctttcc tttgtatgac aatattcttg tccttatgcc aaccggggga tagctgtgga
    16561 cttcgcaacc gaactgctgg tcagggtctt acgtgccagc atctggggaa cgctgctcgg
    16621 ggggttcgcg gcgctcatca ttctggccgg catcacgccg gcccacgccg catcgcccgc
    16681 cttcgtggag ggcgtggtgc tcgtggacta tcaccctagc gccagcgcga ccgaccgcgc
    16741 agcggcacgc ctcgccgtcg gcgccgttcg ggccacgcgc ctgtcgccgc tggccgggcg
    16801 ggcggagaag atcatcctgc ccgcaggcaa aggcgtggag cgtgcgctca aggcgctgcg
    16861 ccgacatccg gcggtacgtg tggcggagcc cgactacgtg ctcacccaca acgccgcagc
    16921 caacgacccg ctctacctgg acggtagcct ctggggcatg tacggcaacg catccactcc
    16981 ttcgaacacc tatggcagcc aggctgcgga ggcctggggc gcgggccaca cgggctcggc
    17041 cggcgtctac atcggcgtga tcgacgaggg cctgcagtac acccatgagg atctcgacgc
    17101 caacgtctgg accaatccag gcgagatccc cgacaacaat agcgacgacg acggcaacgg
    17161 cttcgccgac gacgttcacg gctgggattt ctacaacaac gacgccagcg tctacgacgg
    17221 caccggcgac gaccacggca cccacgtcgc cggcaccatc ggcggcgagg gcggcaacgc
    17281 agtgggcgtg gccggcgtga cctgggacgt gacgctcatt tcggccaagt tcctgggccc
    17341 cagcggcggt tccacctccg gcgccatcgc cgcggtggac tacatcacca tgctcaagac
    17401 gaccaaaggc ctcaacatcg tcgccaccag caactcctgg ggcggcggcg gcttcaacca
    17461 gctgctgctg gacgccatca acaggggcgg cgaccagggc atcctgttcg tcgctgccgc
    17521 cggcaacagc agccgggaca cggacgccgg caactactac ccgtcgaact acgaatgcac
    17581 gacggcttcc cgcagctggg actgcgtgat ctcggtggca tccatcacct ccagcggcac
    17641 catgtcgtcc ttctccaact ggggcgcgac cacggtggac atcggcgcgc ccggctccgc
    17701 catcgaatcc acgctgccca acaacggtta cggcagctac agcggcacgt ccatggccac
    17761 gccacacgtg agcggcgcgg tggctctatg cagctccata gataccaaca tgacggccta
    17821 cgatcttcgc aacgccgtgc tgggcaccgc ggcggccacg gcgtccctcg ccggcaagac
    17881 caccaccggc ggccggctgg acgtcggcac catggtgagc gtgtgcacgc cgccgacgag
    17941 cggcgtctcc ggcgcgccca gcagcctcag cgcgacagcc accagcacat cgtcaattgc
    18001 gctcagctgg accgatggag tcagcggcga ggatcgctgg gaagtgcggc gcggcgcggg
    18061 caactgcacc ggcatggaaa ccgtaggcct cgcgccgccg gattcgacca cattc
//
LOCUS       DBNHLM010000050        10205 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_66588, whole genome shotgun sequence.
ACCESSION   DBNHLM010000050 DBNHLM010000000
VERSION     DBNHLM010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10205)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10205
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_66588"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /gene="selB"
                     /locus_tag="AC16QF_02600"
     CDS             complement(<1..919)
                     /gene="selB"
                     /locus_tag="AC16QF_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HIR6447471.1"
                     /translation="MIVTLAGHVDHGKTSLVRCLTGQDTDRLEEEKRRGLTIDLGFAY
                     VSGDHEVDPIGFVDVPGHHRFIHNMVAGVASMQYALMVVAADDGPMPQSREHLQILEL
                     LGISQGAIALTKCDRVDQARLREAEDEIRDLTADSFLADAPIFRTSATSNEGISELLS
                     HLQKVNGEFRVDQPPRQFRLAVDRAFTVRGSGLIATGTVHAGSIVNDDELAIFPAAVR
                     ARVRGIRTQSREAKEARAGDRCALNLAGVSLDQVGRGSWLSASSFEGSRDLTLELRVV
                     ADFPRPVKHWTPVHVYHATSHATGRIALLD"
     gene            complement(916..2283)
                     /gene="selA"
                     /locus_tag="AC16QF_02605"
     CDS             complement(916..2283)
                     /gene="selA"
                     /locus_tag="AC16QF_02605"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HIR6447472.1"
                     /translation="MPRLRDLPSIDRLLRSPKLAFLVHAHGAETIKQSLRSIQAAWRA
                     TGEVPEWATDPEEYARRLEDQLAGASYRPVFNLTGTMIHTNLGRALLDPEMFRSIEPL
                     VTRPVNLEFDLASGRRGDRDRVVESRLQVLTGAPAATVVNNNAAALMLCLNTAALGRE
                     VIVSRGELIEIGGSFRLPELMERAGCRLVEVGTTNRTHLRDYQAAIGERTGMLLKVHP
                     SNYHISGFTHDVGVAELAALAREHRLPLCVDLGSGTLVDLRKWGLPHEPMPQEILAQG
                     ADLVTFSGDKLLGSVQAGIIVGDSENVGAIKRNPMKRALRPDKLTLAFLDRTLRLYEN
                     PERLAERLPLLRSLTRTLGELENQGEVLARSLETVLGDFDVRLVPSEVQIGSGSLPNQ
                     RIASRAVRITHDDPSEVRRLGERLRRLATPVIGRLHQEAVWLDVRGAEPLDELIEVLH
                     GLAAP"
     gene            complement(2337..3629)
                     /gene="gltA"
                     /locus_tag="AC16QF_02610"
     CDS             complement(2337..3629)
                     /gene="gltA"
                     /locus_tag="AC16QF_02610"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017518059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0036440 - citrate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR6447473.1"
                     /translation="MADKSGRTAQLTIDGRTIELPMLEGTLGPEVIDVGKLTAQGYFT
                     YDPGFVSTASCESAITYIDGDEGILLHRGYPIEQLAEQSDYLELCYLLLNGELPSQAE
                     KADFVHTICHHTMVHEQLNQFFRGFRRDAHPMAIMCGVTGALSAFYHDALDINNHQHR
                     KITAHRLIAKMPTLAAMSYKYAHGEPFVYPRNDLAYAENFLHMMFATPCEDYEVNPII
                     ARAMDRIFLLHADHEQNASTSTVRLAGSTGANPFACIAAGIAALWGPAHGGANEAVLN
                     MLDEIGDEKHIDKYVKRAKDKDDPFRIMGFGHRVYKNYDPRAKVMQQTCHEVLDELGV
                     ENDPVLRMAQRLEKIALEDEYFVERKLYPNVDFYSGIILKAIGIPTEMFTVIFATGRT
                     PGWIAHWNEMIGAPYKIGRPRQLYQGATPRDYVPLAER"
     gene            3942..4316
                     /gene="sdhC"
                     /locus_tag="AC16QF_02615"
     CDS             3942..4316
                     /gene="sdhC"
                     /locus_tag="AC16QF_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013307.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HIR6447474.1"
                     /translation="MKTERPVYLSLTQFGWPVTAIASITHRITGVLLFVGISYLLWLL
                     SLALNSPEGFAAATDVLGAPLPKLLLIAVIAAVLYHLFAGIKHMFMDFHVGDSFEAAR
                     ANSLVVFVLTAVATVLVGVRLW"
     gene            4310..4681
                     /gene="sdhD"
                     /locus_tag="AC16QF_02620"
     CDS             4310..4681
                     /gene="sdhD"
                     /locus_tag="AC16QF_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HIR6447475.1"
                     /translation="MVTNVTSLTRSGLSDFVVQRVSAVIIGLYSLCVLGFFLIYPDVT
                     RDQLLAYLGGMPMKVFSTLTVLATAAHAWIGMWTVGTDYIREHYFGKHATSFRLVYQA
                     GVLAILFAYVAWALQLFWSVG"
     gene            4678..6450
                     /gene="sdhA"
                     /locus_tag="AC16QF_02625"
     CDS             4678..6450
                     /gene="sdhA"
                     /locus_tag="AC16QF_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HIR6447476.1"
                     /translation="MTRMRKMEFDGVIVGGGGAGMRASLQLARSGLKTAVISKVFPTR
                     SHTVSAQGGITCAIASDDPNDDWRWHMYDTVKGSDYIGDQDAIEYMCSQGPQAVFELE
                     HMGLPFSRTEQGRIYQRPFGGQSKDFGKGGQAARTCAAADRTGHTLLHTLYQANLRAN
                     TTFLNEWFAVDLVKNQDGAVVGIIAIEMETGETVFVSSKATVLATGGAGRIYASTTNA
                     HMCTGDGAGMALRAGFPMQDMEMWQFHPTGIAGAGTLVTEGCRGEGGYLINKDGERFM
                     ERYAPTAKDLAGRDVVARSMILEILDGRGCGPDGDHVMLKLDHLGEEVLNSRLPGILE
                     LSRTFAHVDPVKEPIPVVPTCHYMMGGIPTGVLGQALTQDAQGDDREIPGLYAAGEAA
                     CVSVHGANRLGGNSLLDLIVFGRAAGLHIEEVMRQGVSYRDPSDSDLEQAQARLNRWD
                     ESGPGETVADLKKELQTTMQNSFGVFRTGTHMEEGIKKIAELRDRISRAHLGDKSKVF
                     NTARLEALELDNLLEVAEATAIAANARTESRGAHAREDFQVRDDENWLCHSLFFSDER
                     RVTKRAVNFSPETVDTFQPMERKY"
     gene            6459..7166
                     /locus_tag="AC16QF_02630"
     CDS             6459..7166
                     /locus_tag="AC16QF_02630"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010166250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HIR6447477.1"
                     /translation="MMQVSVYRYNPETDEVPAMREMSIELPGDRDLMVLDVLEMLKAE
                     DPTLSYRRSCREGVCGSDGINMNGRNGLACITPVSSVAKRGKLVLRPLPGLPVIRDLV
                     VDMTQFYRQYEKIKPYLINDKPPPAQERLQSPEERAELDGLYECILCACCSTACPSFW
                     WNPDKFIGPAGLLQAYRFLADSRDTATAERLSELDDPFSVFRCRGIMNCVSVCPKGLN
                     PTRAIGKIRTMLLQQGT"
     gene            7255..10092
                     /locus_tag="AC16QF_02635"
     CDS             7255..10092
                     /locus_tag="AC16QF_02635"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018925108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HIR6447478.1"
                     /translation="MADSFRERMERSSHLAGANAAYIEAMYERYLDEPNEVPPEWREY
                     FDTLPRVEGAVGADVPHSTIVDQFERLGRNRLKARPERESHFAQNVHEQKQMRVQDLI
                     SAYRHRGHKKANIDPLGMMERPAMPILDLAYHHLSLADLDETFNVGSFHYGSPEAKLR
                     DLVDALERTYCTSIGFEYMHIVDQSEQLWVQQRLESVRARPVLTEDHKRVILERLTAA
                     EGLEKYLHGRYPGTKRFGLEGGESLIPMLHELLQRLGSHGVLEAVIAMAHRGRLNVLV
                     NILGKDPRLLFDEFDGKLPVNEDDMGDVKYHQGFSSNVMTPGGEMHLALAFNPSHLEI
                     VGPVVEGSVRARQDRRRDHVGTRVVPILVHGDASFAGQGVVMETLQMSQTRGFKTGGT
                     IHIIINNQIGFTTHRQDDARSTEYCSEVAKLVQAPIFHVNGDDPEAVLFASQLAADYR
                     MEFKKDVVIDLVCYRRRGHNEAEEPMKTQPLMYKKIRQHPTTRAIYVERLIGEGLVED
                     SEADGLAEAYRNTLDQGGHVALSLVKEPNTELFVDWTPYLGHDTVMPADTRVDLSVIQ
                     ALARRLEELPDGFVLQRQVQKIMEDRHKMSFGAMPINWGFAETMAYATLLDEGYGVRL
                     TGQDVGVGTFSHRHAALHNQKDGKRYIPLNSLSETVNFDLYDSLLSEEAVLAFEYGYA
                     ATAPDKLVIWEAQFGDFANGAQVVIDQFISSGEVKWARLCGLTMLLPHGYEGAGPEHS
                     SARLERFLQLCAQHNMQVCLPTTPAQVFHMLRRQVVWPARKPLVALTPKSLLRHKLAV
                     STLEDLANGTFHTVLGEVDEIDPDGVERVVLCSGKVFYELLEARREANASQVAIIRLE
                     QLYPFPEAELERIMAPYGSVRDVVWCQEEPMNQGAWYASQHHMRRVIMKHNENIYLAY
                     AGRPAFAAPASGHAHLHGVRQQRLVEEALFG"
BASE COUNT         1840 a         3160 c         3319 g         1886 t
ORIGIN      
        1 gatcgaggag ggcaattctg cccgtggcgt gtgaggtggc atgatagacg tgcacgggag
       61 tccagtgctt gaccgggcgc ggaaagtcgg cgacgacccg cagctcgagc gtgaggtcgc
      121 ggctcccttc gaagctgctt gctgagagcc agctaccgcg acccacctgg tccagggaga
      181 cgcctgccag gttcagcgcg cagcggtccc cggcccgggc ctctttggct tcgcggctct
      241 gggtacggat ccctctcacc cgagcgcgca cggcggcagg aaagatcgcc agctcatcgt
      301 cgttgacgat ggatccggcg tgaacggtgc cagttgcgat caggccggaa cccctgacgg
      361 tgaacgcgcg gtcgacggcg agccggaatt ggcgcggggg ctgatccacg cggaactcgc
      421 cgttgacctt ctggaggtgg ctgagaagct cggagatgcc ctcgttggac gtggctgacg
      481 ttcgaaatat gggggcgtct gccaggaagg agtcagcggt cagatcgcga atctcatctt
      541 cggcttcgcg gagcctggcc tgatcgaccc gatcgcattt ggtcagggca atggctccct
      601 gggagattcc cagcagctcc agaatctgca ggtgctcgcg gctctggggc atgggcccgt
      661 cgtcggccgc gacgaccatc agggcgtact gcatggacgc tacccctgcc accatgttgt
      721 ggatgaaccg gtggtggccg ggcacgtcga cgaagccgat gggatccacc tcgtggtcgc
      781 cgctgacgta ggcaaagccc aggtcgatcg tgagcccgcg gcgcttttct tcttcgagcc
      841 ggtccgtgtc ctgccccgtc aagcagcgga ccaggctggt tttgccgtgg tcgacgtgcc
      901 cggcaagggt aacgatcacg gcgcggcgag cccgtgcagc acctcgatga gctcgtcgag
      961 cggctcggcg cctcgaacgt ccagccagac ggcctcctga tgaagcctgc cgatcaccgg
     1021 cgtggctagc ctacgcagac gttcgccgag cctgcggacc tcggaggggt cgtcatgggt
     1081 gatgcgcact gcccggctcg cgatgcgttg gttgggcagg cttccgctac cgatctgcac
     1141 ttcgctgggc accaggcgca cgtcgaagtc gcccagaacg gtctccagtg atcgagccag
     1201 cacttcgccc tggttttcca gctcgcccag ggtgcgggtg agcgagcgca ggaggggaag
     1261 cctttcggcc agacgctcgg ggttttcgta gagcctgaga gtccggtcca gaaaggccag
     1321 ggtcaacttg tccggacgca gagcgcgctt catggggttt cgctttatcg cgccgacgtt
     1381 ttccgagtcg cccacgatga tgcccgcctg aacggagccc agcagcttgt ctccgctgaa
     1441 cgtcaccaga tctgcgccct gggcgagaat ctcttgaggc atgggttcgt gaggcagccc
     1501 ccacttccgg aggtcgacga gcgtgccgct gccgaggtcc acacacaggg gcaaccggtg
     1561 ctcccgtgcc agagccgcca gctccgcgac gccgacatcg tgggtgaaac cgctgatgtg
     1621 atagttggat ggatgcactt tcagcagcat tcccgttcgc tcgccgatcg cagcctggta
     1681 gtcgcgaagg tgggtgcggt tggtggttcc cacctccacc aggcgacacc cggcgcgctc
     1741 catcagctcg ggaagcctga agctgccgcc gatctcgatg agctcgccac gggaaacgat
     1801 cacctcccgc ccgagcgcgg ccgtgttcag gcacaacatc agggcggccg cgttgttgtt
     1861 gaccacggtg gcggccggag caccagtgag cacctgcagc cgggactcga ccacccgatc
     1921 ccggtcgcct cgccggccgc tggcaagatc gaactccagg ttcacgggcc gggtcaccag
     1981 aggctcgatg gaccggaaca tctccggatc gagcagagcg cggcctaggt tggtgtggat
     2041 catcgtcccg gtgaggttga ataccgggcg ataggaggcg ccggcgagct gatcttccag
     2101 ccgccgcgcg tactcctcgg gatccgttgc ccactccggg acctcgccgg tggcccgcca
     2161 ggccgcctgt attgatctca atgactgctt gatggtttcc gcgccgtggg cgtggaccag
     2221 gaaggcgagc ttcggggagc gaagcagccg gtcgatgctg ggcaggtcac gcagtcgtgg
     2281 catcgtcgat ctctcggcgg agagtccccg gcgcggaaaa cgggccggga cctttgtcag
     2341 cgctcggcga gggggacgta gtcccggggc gtcgcgccct ggtagagctg tcgcggtcgt
     2401 ccgatcttgt aaggcgcgcc gatcatctcg ttccaatggg caatccagcc aggggtgcgg
     2461 ccggtggcga agataacggt gaacatctcg gtgggaatgc cgatggcctt gaggatgatc
     2521 cccgagtaga agtcgacgtt ggggtacagc ttgcgctcga cgaagtactc gtcctccagc
     2581 gcgatcttct ccaggcgctg agccatgcgc agcaccggat cgttctccac gccgagctcg
     2641 tcgagcacct catgacacgt ctgctgcatg accttggcac gcggatcgta gttcttgtac
     2701 acccggtggc cgaaacccat gatccggaac gggtcgtcct tgtccttggc gcgtttgacg
     2761 tacttgtcga tgtgcttctc gtcgccgatc tcatcgagca tgttgagcac ggcttcgttg
     2821 gcccctccgt gcgcggggcc ccacagtgcg gcaatgccgg cagcgatgca cgcgaagggg
     2881 ttggcgcccg tggagccggc cagcctgacg gtggacgtcg aggcattctg ctcatggtct
     2941 gcgtgcagga ggaaaatacg atccatcgcg cgggcgatga tcgggttgac ctcgtaatcc
     3001 tcgcacggcg tggcaaacat catgtgcagg aaattctcgg cgtaggcgag gtcgttcctg
     3061 gggtagacga agggttctcc gtgagcgtac ttgtagctca tggcggccag ggtcggcatc
     3121 ttggcgatga gccggtgcgc cgtgatcttc cggtgctggt ggttattgat gtcgagggcg
     3181 tcgtggtaga aggccgaaag cgcgccggtg accccgcaca tgatcgccat cggatgggca
     3241 tcgcgacgga acccgcggaa aaactggttg agctgctcgt ggaccatggt gtggtggcag
     3301 atggtgtgca cgaaatcggc tttctccgcc tggctgggca gctcgccgtt gagcagcagg
     3361 tagcagagct ccagatagtc ggactgctcg gccagctgct cgatggggta tccccggtgc
     3421 agcaggatgc cctcgtcgcc gtcgatgtac gtgatggccg attcgcagga ggccgtcgag
     3481 acgaagcccg gatcgtatgt gaagtatccc tgtgccgtga gcttgccgac gtcgatgacc
     3541 tcggggccca gcgtgccctc tagcattggc agttcgatgg tcctcccgtc aatcgtcagc
     3601 tgcgccgtac ggccactctt gtccgccatt gagacctccc taagaaactg ctacgtgccg
     3661 ggctctgtgg cccggtttaa cgtcgaatcg gcaggtgagc gtattgacgg caccagccgt
     3721 tgtcaattcg ctcggaaccc cagatgtgat tggggtccag gcattcgcct ggcccctgcc
     3781 cgaatggtct caaatagccc cgatttcttg gcttttaccc ccacacttga ctaaaatgcg
     3841 gccgcgaatt cggccactgc gcgtggcctg cggctcctcc ccgcttaaat tccgaagacc
     3901 gtcttgacaa cttagcaacc gaagcggatg tggaaaggga tatgaagacc gagcgtccgg
     3961 tatatttgtc gctgacccag ttcggctggc ccgtaacggc cattgcctcc atcactcatc
     4021 ggatcaccgg cgtgctgctg ttcgtcggta tctcttacct tctatggcta ctgagcctgg
     4081 cgctcaactc gccggagggc tttgctgcgg cgaccgacgt gctcggcgcc ccgcttccca
     4141 agctgttgct gatcgcggtg atcgccgccg tcctctacca cctgtttgcc ggaatcaagc
     4201 acatgttcat ggactttcac gtgggcgatt ccttcgaagc cgcccgggcg aactccctgg
     4261 tcgtgttcgt tctcaccgcc gttgcgacgg tgctggtggg ggtgcggcta tggtaacgaa
     4321 cgtaaccagc ctcactcgga gcggtctttc agatttcgtc gtccagcggg tgtccgctgt
     4381 gatcatcggt ctttacagcc tgtgcgtgct gggcttcttc ctgatctatc cggacgtgac
     4441 gcgggaccag ctgctggctt acctgggtgg gatgccgatg aaggtattca gcaccttgac
     4501 cgtcctcgcc accgccgcgc acgcctggat aggtatgtgg acagtgggga cggattacat
     4561 tcgggagcat tacttcggta agcacgcgac cagctttcga ctggtttacc aggcaggcgt
     4621 gctggcgatt ctgttcgcct atgtcgcctg ggccctgcaa ctgttctgga gcgtgggatg
     4681 acgagaatgc gcaagatgga gtttgacggc gtcatcgtcg gtggcggcgg cgcgggcatg
     4741 cgggcgtctc ttcagctggc gcggtcgggc ctgaaaacgg ccgtgatctc caaggtattt
     4801 ccgacgcgct cccacacggt ttccgcccag ggtgggatca cgtgcgccat cgccagtgac
     4861 gatccgaacg atgactggcg ctggcacatg tacgacacgg tgaaggggtc tgactacatc
     4921 ggtgatcagg atgccatcga gtacatgtgc agccagggac cccaggcggt tttcgagctc
     4981 gagcacatgg ggctgccgtt ttcccgaacc gagcagggcc gcatctatca gcggcccttt
     5041 ggcgggcagt cgaaggattt cggcaagggc ggccaggcgg cccgaacctg cgcggccgct
     5101 gacaggacgg gtcataccct tctgcatacg ctctaccagg ccaacctgcg ggcaaacacc
     5161 acatttctga acgaatggtt cgccgtggat ctggtcaaga accaggacgg cgccgtcgtc
     5221 ggcatcatcg ccatcgagat ggagaccgga gaaacggtct tcgtcagctc caaggccacc
     5281 gtgctggcca cgggaggcgc gggccggatc tacgcgagca ccaccaacgc tcacatgtgc
     5341 accggcgatg gagccggaat ggcgctcagg gccggtttcc ccatgcagga catggagatg
     5401 tggcagttcc atcccaccgg aatcgccggg gccggtaccc tggtgaccga gggttgccgc
     5461 ggcgaaggcg gctacctcat caacaaggac ggcgaacgct tcatggagcg ctacgcgcct
     5521 actgcgaagg atctcgcggg ccgcgacgtg gtggcccggt caatgatcct ggagattctc
     5581 gacggtcgcg gttgtggccc ggacggggac cacgtgatgc tcaagctcga tcatctggga
     5641 gaagaggtgc tcaacagccg cctgccggga atcctcgagc tgtcccgcac atttgctcat
     5701 gtggatcccg tcaaggagcc gatccccgta gttcccacct gccattacat gatgggcggt
     5761 attcccaccg gcgtgctcgg ccaggcgctt acccaggacg cccaaggcga cgaccgcgaa
     5821 atccccggtc tttatgctgc tggagaggcc gcatgcgtct ccgtgcatgg cgccaaccga
     5881 ctcggcggca actcgctgct cgacctcatc gtgttcggtc gggccgcggg gctgcacata
     5941 gaggaagtga tgcgtcaggg cgtttcctat cgggatccca gcgacagtga tctcgagcag
     6001 gctcaggccc gtctgaaccg ctgggacgaa agtggtccgg gcgaaacggt cgccgacctc
     6061 aagaaggagc tgcagacgac gatgcagaac agcttcggcg tgttccggac cgggactcac
     6121 atggaagagg gcatcaagaa gattgcggag ctgcgcgatc gcatctctcg agcccatctc
     6181 ggcgacaaga gcaaggtctt caacacggcg cggctagaag cccttgagct ggacaacctg
     6241 ctcgaggtgg cagaggccac cgccattgcc gccaacgccc gcacggagag ccgaggcgct
     6301 cacgcccggg aggacttcca ggtgcgcgac gacgagaact ggctttgcca ctcgctgttt
     6361 ttctcggacg aaagacgggt taccaagcga gccgtgaact tcagccctga gaccgtggac
     6421 accttccagc ccatggaacg gaagtactag agaggatggt gatgcaggtt agcgtttatc
     6481 gatacaaccc ggagaccgac gaggtcccgg ccatgcgcga gatgagcatc gagctccccg
     6541 gcgaccggga tctgatggtt ctcgacgttc tcgagatgct gaaggccgag gatcctaccc
     6601 tgtcctaccg gcgctcctgc cgggagggtg tttgcggttc ggacggcatc aacatgaacg
     6661 ggcgaaacgg gctcgcctgc atcacgccgg tttcctctgt tgcaaagcgg ggcaagctcg
     6721 ttctgcggcc gcttcccggg ctgccggtga tccgggacct ggtggtggac atgactcagt
     6781 tctatcgcca gtacgaaaag atcaagccgt acctcattaa cgacaagccg ccccctgccc
     6841 aggagcgcct gcagagtccg gaggagcgtg ccgagctcga cgggttgtac gagtgcatcc
     6901 tgtgtgcctg ctgctccacc gcctgcccct cgttctggtg gaaccctgac aagttcatcg
     6961 gtccggcggg tctgttgcag gcctatcggt tcctggccga cagccgggac acggcgacag
     7021 ccgagcggct ctcggagctg gacgacccct tcagcgtctt ccgatgccgc gggatcatga
     7081 actgcgtgag cgtttgcccc aaggggctca accccacgcg ggccatcggc aagatccgca
     7141 ccatgcttct gcagcagggg acgtagacga accccttgcg cggctatact gctgcttcaa
     7201 cggacaaaag gctgacggct ctcacggtga gctcctatcc cacgcaggct gacaatggct
     7261 gactcgtttc gcgagcgcat ggagcgtagt tcgcacctgg ccggtgccaa cgctgcctac
     7321 atcgaggcca tgtacgagcg ctatctcgac gaaccgaacg aggtgccccc tgagtggcgc
     7381 gagtatttcg acacgctgcc gcgcgtggaa ggggctgtcg gtgccgatgt gccccacagc
     7441 accatcgtcg atcagttcga gcggctggga cgcaaccgcc tgaaagcgag accggagcgc
     7501 gagagccact ttgcgcagaa cgtgcacgag cagaagcaga tgcgcgttca ggatctcatc
     7561 tcggcctatc ggcaccgggg ccacaagaag gcgaacatcg acccgctggg catgatggaa
     7621 cgacccgcca tgcccatcct cgatctggcc tatcaccatc tctccctcgc ggatctggac
     7681 gagacgttca acgttggatc ttttcactac ggatcaccag aggcgaagct gcgcgatctc
     7741 gtggatgccc tcgagcggac ctactgcacg tccatcggtt tcgagtacat gcacatcgtc
     7801 gatcagagcg agcagctctg ggttcagcag cgcctcgaaa gcgtgcgtgc gcggccggta
     7861 ctgaccgagg atcacaagcg ggtgatcctg gaacgcctga ccgctgcaga aggcctggaa
     7921 aagtacctgc acggccgtta tcccggtacc aagcgtttcg gcctggaagg cggggagagc
     7981 ctcatcccca tgcttcacga gctgctgcag cggctgggtt cccatggcgt cctcgaggcc
     8041 gtcatcgcca tggcgcatcg gggccggctc aacgtgctgg tcaacatcct gggaaaagac
     8101 ccccggctcc ttttcgatga gttcgacggc aagctgccgg tgaacgaaga cgacatggga
     8161 gacgtcaagt accaccaggg attttccagc aacgtgatga cgccgggcgg cgagatgcac
     8221 ctggcgctgg cgttcaatcc ttcccacctg gagatcgtcg ggcccgtggt ggagggatcc
     8281 gtgcgggcga ggcaggatcg ccgccgcgac catgtgggaa cccgggtcgt gcccatcctc
     8341 gttcacggcg acgcgtcctt cgccgggcag ggagtggtca tggagaccct gcagatgtct
     8401 cagacccgag ggttcaagac gggcgggacg atccatatca tcatcaacaa ccagatcggc
     8461 ttcaccaccc atcgtcagga cgacgcgcgc tccaccgagt actgctcgga ggtggccaag
     8521 ctggttcagg cccccatctt ccacgtcaac ggcgacgatc cggaggcggt gctgtttgct
     8581 tcgcaacttg ccgccgatta ccggatggag ttcaaaaagg acgtggtgat cgatctcgtc
     8641 tgctatcgcc gcaggggtca caacgaggcc gaagagccca tgaagaccca gccgctgatg
     8701 tacaagaaga tccgtcagca cccgaccacc cgggcgatat acgtggaacg cctgataggg
     8761 gaagggctcg tggaggacag cgaggccgat ggccttgccg aggcgtaccg gaacaccctg
     8821 gatcagggag ggcacgtggc cctgagcctg gtgaaggaac cgaacaccga gctgttcgtg
     8881 gactggacgc cctatctggg ccacgacacc gtcatgcccg cggatacccg cgtcgatctc
     8941 agcgtgatac aggcgctcgc caggcggctc gaggagctcc ccgatggttt cgtgcttcag
     9001 cgccaggtcc agaagatcat ggaagatcga cacaagatga gcttcggggc gatgcccatc
     9061 aactggggat ttgccgagac catggcgtac gcaacgctgc tggacgaggg ttacggagtc
     9121 cgcctgacgg gccaggacgt gggcgtcggg accttttctc accggcatgc cgctctccac
     9181 aaccagaagg acggcaagcg ctacatcccc ctgaacagcc tctctgagac cgtcaacttc
     9241 gacctctacg acagcctgct ttcggaagag gcggtgctcg ccttcgagta cggttacgcc
     9301 gcgaccgcgc cggacaagct tgtcatctgg gaggcccagt tcggtgactt tgccaatggt
     9361 gcccaggtgg tcatcgacca gttcatctcc agcggcgagg tgaaatgggc ccggctgtgc
     9421 ggtttgacca tgctgctgcc ccacggctac gagggcgccg gacccgagca ctcgtcggcg
     9481 aggctcgagc ggtttctcca gctctgcgcc caacacaaca tgcaggtctg ccttcccacc
     9541 acgccggctc aggtgtttca catgttgcgt cgtcaggtgg tctggcccgc gcgcaagccg
     9601 ctggtggcgc tcacgccaaa gagcctgctc aggcacaagc tcgccgtgtc gacgctcgag
     9661 gacctggcga acgggacgtt tcacacggtg ctgggcgaag tcgacgagat agatccggac
     9721 ggcgtcgagc gcgttgtgct ctgcagcggg aaggttttct acgagctgct ggaagcccgt
     9781 cgggaggcga acgcgagtca ggtcgccatc atcaggctcg agcagctcta cccgtttccc
     9841 gaggcggagc tcgagcgcat catggcgccg tacggctcgg tacgggacgt ggtctggtgt
     9901 caggaggaac ccatgaacca gggtgcctgg tacgcctccc agcaccatat gcgccgggtc
     9961 atcatgaagc acaatgaaaa catctacctg gcctacgccg ggcgcccggc tttcgccgcc
    10021 ccggccagcg ggcacgcgca tctgcacggc gtgcggcagc agcgcctggt ggaggaggcc
    10081 ctgttcggct aacgggctat gaaggaccgc cgatgaccat ccaaatcaaa gcgccaacgt
    10141 ttccggaatc catcgccgac gggaccgtag ccgcctggca ccggaacgtg ggcgatcagg
    10201 tccag
//
LOCUS       DBNHLM010000051        25336 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1143615, whole genome shotgun sequence.
ACCESSION   DBNHLM010000051 DBNHLM010000000
VERSION     DBNHLM010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 25336)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 25336)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25336
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1143615"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="AC16QF_02640"
     CDS             <1..1012
                     /locus_tag="AC16QF_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447479.1"
                     /translation="RPFFSGLMSTHPPLEDRIRAIEPRWDGRLLERGQPSPTRPAGAA
                     GFAGTGAAGIQAPGGISVGVPAESIADEVGRLDQTSLDNAVGIIASIDPKLREAAHDP
                     WSGRGLIYATLTHADGYARDRQLAYLEENAEPGVPEHVFRLVPAVRHLDEPRKLALVE
                     MAVPALKELSDPQFRRFIDNVVAMIRSDRQIDLFEWVMHRLLIKELRPHFDGPERIRA
                     RYRSPDQVADAALTVLSALARSGHGDVGAQAKALATGAELLDLAPRMDTEDDPNFVRL
                     GQALWQLRHLKPLVKPRVLKACAAVALADGQVSGREGALLQGIAATLDCPLPPSIYAP
                     KS"
     gene            1087..2613
                     /locus_tag="AC16QF_02645"
     CDS             1087..2613
                     /locus_tag="AC16QF_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR6447480.1"
                     /translation="MTEATLNYGDVTQLAALDLGSNSFHLIVAQETNGRLQIIDKIKE
                     MVRLANGLDEKNELTGPATDTALACLERFGQRLNSLQRRNVRVVGTNTLRRARNAAAF
                     IERAEQALGHPVEIISGREEARLIYLGVSHSIEDNYERRLVIDIGGGSTELILGRHFE
                     AEKTESLHMGCVGISRQFFGDGRIRAGQFRAAVNHARKELVPVAGTYGRKSRDTVIGA
                     SGTILAAQAVLAEMDGSRDISLDGLERVREALIDAGHVDKLALPGLPPERAPVFPGGL
                     AVLIAAFKQLDIASMEATSGALREGLLYDLLGRVHHQDVRENSVADLCMRYHVDQTHA
                     RRVREMALALLAQVALEWDLTEPRHQLLLGWAADLHEIGMDIAHSQYHRHSGYLLANM
                     DLPGFSKGDQLELAALVRAHRRKFQQEDGDYPPHALRLAVLLRLAVLLHRNRANTALP
                     HVALRADGDALLLKFPRGWLKRHPLTRLDLDQEADYLSAVGVGLRIDENENAARSVAG
                     "
     gene            complement(2599..4686)
                     /gene="ppk1"
                     /locus_tag="AC16QF_02650"
     CDS             complement(2599..4686)
                     /gene="ppk1"
                     /locus_tag="AC16QF_02650"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR6447481.1"
                     /translation="MSANPSTDFAGLANPELYINRELSLLEFHRRVLAQAQEERVPLL
                     ERLRYLCISSSVLDEFFEIRVAGLKQQEAYRATQRGPDNLSPSEQLRRIFETVRELVE
                     EQYRVLNEDLLPSLDDQGIRILDPDEWSSKQRQWLKRYFKRELAPIMSPIALDPAHPF
                     PQPLNKSLTFIVTLEGEDAFGRRSGKAIVQAPRALSRVVRLPESCAATPNEFVLLSSI
                     IEVHVGDLFPGMKSTGCFQFRVTRNSDLFVDMEEVDDLLRALEGELSSRRFGDAVRLE
                     VARDCPDPLEAFLTGQFHLKPEDVYRCSGPVNLMRLATVPDLVDRPELKFPGFTPGIP
                     DRLRNANDMFEVIRQGDVLLHHPFESFAPFIDFLRQAAADPQVLSIRQTLYRTGTESA
                     VVEALRDAATNGKEVLVVIELRARFDEEANIELANYLQRAGAQVVYGVVGHKTHAKMS
                     MVIRREGRPLRRYVHLGTGNYHTRTTRLYTDYGLFTCDPDIGDDVQQLFQQLTSMGKT
                     SGLKKLLQAPFTMHSSMLVFIEREAENARAGRPARILAKMNSLIEPQVIQALYAASQA
                     GVKIDLVVRGVCGLRPGIEGVSENVTVRSIIGRFLEHTRVFYFENEGDPRVYLSSADW
                     MNRNFFDRVEACFPIEDASLRDRIYRELGMYLGDNCQSWMLAADGKYRRLVPPEGERR
                     SAQEGLLEELAGD"
     gene            4779..5507
                     /gene="cmoA"
                     /locus_tag="AC16QF_02655"
     CDS             4779..5507
                     /gene="cmoA"
                     /locus_tag="AC16QF_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003124239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HIR6447482.1"
                     /translation="MSSRDRVYRESKRQIVDFAFDEAVADVFPDMIRRSVPGYETVIP
                     ITGMLAARHLEHMPDERRLAYDLGCSLGATTTAVLDQLDERPARVIAVDNAAAMIARA
                     VDAIRDPRARFEVADVTMLEFEPAGVVLMNFVLQFIDPTQRVPLLKRIRQALEPDGLL
                     IVSEKLRFEDPYTQALFERAHLDYKRANGYSELEISQKRSALEKVMIVDTEAEHRSRF
                     EAAGFATTNRWFQCLNWVSYLVAP"
     gene            5504..6439
                     /gene="cmoB"
                     /locus_tag="AC16QF_02660"
     CDS             5504..6439
                     /gene="cmoB"
                     /locus_tag="AC16QF_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HIR6447483.1"
                     /translation="MIFEPVLDLIPEWRRLGPDLNARLSTKGHGDYPRWEAALAALPD
                     LQAEQAALSDRVTVSGNASEDQRRDLRESLMALHPWRKGPFSLFEVDIDCEWRSDWKW
                     LRVLPHLDSLSGRRVLDVGCGNGYYGWRMLEAGATAVFGVDPTLVFCMQHLAVSRYLS
                     GAANWVLPLRFEQLPPHPFDTVFSMGVIYHRRDPAEHARRLAEFTRPGGQVVVESLVV
                     EHGRSLKPVGRYARMRNVHVVPTTKRLARWLKDAGFVDPELVDLTPTTVAEQRATTWM
                     RFESLEDALDPKMRNRTIEGHPAPVRAVMVARKPV"
     gene            6461..6892
                     /locus_tag="AC16QF_02665"
     CDS             6461..6892
                     /locus_tag="AC16QF_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447484.1"
                     /translation="MRFETAYGLKTAKPQNTTMMLGIIPISFRFRGKIMRLEDMYHCF
                     REHPGTGLHPTQISRYTGLGLAEVNARLEATPELFVRLPKRPDGITRYRLTSATTAKA
                     PEDVEKFLRQAARRESLMLYAVGSMVLLILLIVVILVGPAV"
     gene            6893..8002
                     /locus_tag="AC16QF_02670"
     CDS             6893..8002
                     /locus_tag="AC16QF_02670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6447485.1"
                     /translation="MPELLDDKQRALREHAAAFAREQLEPRRSMDNKQAREEIVRAAK
                     AAGFYAMTQPAEFGGNPASVLELTIVRDELAASNAPHADAVFGPGPGVLAGVGEPLAS
                     DYLGPVLAGTKQGAFGFTEPDDAPGPTSGRLESDELIVSGQKSYVTGGEQADFINTLV
                     QIEGHGPALVVIDTHAAGVIRERTFSSLDGSRHAAFRFEEVRVPRSRVIGKPGEGLPR
                     AMRQIGDTRLAIAAQCVGAMRFVLELLTTHLKAPDRSGRARGSKEGVRLRYADLRIKA
                     YAARSMVYRTARLADSGSNVINEGIACKVFATETAGEIVDTGIQLVGGSALVESHPLA
                     ELYRRLRALRLAEGGSDVLRLNLARGRLDLDKGRI"
     gene            complement(8027..8629)
                     /gene="smrA"
                     /locus_tag="AC16QF_02675"
     CDS             complement(8027..8629)
                     /gene="smrA"
                     /locus_tag="AC16QF_02675"
                     /inference="COORDINATES: protein motif:HMM:NF033154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HIR6447486.1"
                     /translation="MGDEDVFREAMSDVAPLQRKQRAQPERGPVEATSAQLERREFAL
                     GNRDREAADPNYLTLGEVPQRQPREIVEWKKDGVQNAVFARLKAGKYPVEGSVDLHRQ
                     TVREARAAVFRFLNLAMAKGWRMVLIAHGRGEQSETPARIKSYVAAWLAQVPEVIAYH
                     SAQNNQGGTGACYVLLKKSPQARENNREQYGGKGEAEEPG"
     gene            complement(8637..9410)
                     /locus_tag="AC16QF_02680"
     CDS             complement(8637..9410)
                     /locus_tag="AC16QF_02680"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6447487.1"
                     /translation="MESGSSEIDRLEPRAFERDGFVVVRGCCDPGLVRRIRDRASSYL
                     QPLLGPAEFEADVGYPGSPASRASDGGDTCRRLLHAYARDSVFRDWATGSSVGAVLRV
                     LFGGADVMMSQCHHNCVMTKHPGYSSATLWHQDIRYWSFDRPELVSVWLALGEETEEN
                     GALKVIPGSHAMNIDRGRLDRDLFLRPELAENAELVEHAQTVPLEAGDVLFFHCRLFH
                     AAGKNLTDEVKLSVVTTYHTADNRPIPDTRSAQYPGVPL"
     gene            complement(9418..10851)
                     /locus_tag="AC16QF_02685"
     CDS             complement(9418..10851)
                     /locus_tag="AC16QF_02685"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020305637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006081 - aldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HIR6447488.1"
                     /translation="MQDMTLEQMLALLERQREDYLGEGTVSLAKRVDRLERAVGLLKT
                     HEKRLVEAMTADFGHRSEHQSMLTDVAGSIGPLRHAQKHLARWMRPERRKVGPFPLNV
                     LGAKARVEYQPLGVVGVISPWNFPVNLTFTPLAGILGAGNRCMIKPSEYTPATSEVMA
                     EIFPTAFDELEIAVVTGGPQVGADFSGLPFDHLLFTGATSIARHVMRAASENLVPVTL
                     ELGGKSPVVVGRSADIQKTTDAIMTGKMMNAGQICLAPDYVFVPEERVDEFVESSARS
                     VEKMYPTLLDNPDYTSVINQRHYDRLQGYVEEAREKGAKVVEVNPANEDFRQQPHHKI
                     PPTLIVDPDESLQVMQDEIFGPILPVKGYANVADTIDYINHHPRPLGLYYFGDDREEE
                     GKVLSHTTSGGVTVNDVVMHVAQEDLPFGGVGPSGMGAYHGHDGFKTFSHAKSIFTQS
                     KVNVAELAGLRPPYGEKMLKAIRAQMR"
     gene            complement(10964..11224)
                     /locus_tag="AC16QF_02690"
     CDS             complement(10964..11224)
                     /locus_tag="AC16QF_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447489.1"
                     /translation="MPDVVALSHRGKEFEIRLADDGALELHVDGCLRKRREPTGREPQ
                     YVWTNVELQWEEHHYIEARYWAGNRRLKVTVNGDGIYDRSLP"
     gene            11317..12687
                     /locus_tag="AC16QF_02695"
     CDS             11317..12687
                     /locus_tag="AC16QF_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR6447490.1"
                     /translation="MPASPDQPRFARVLKSREVITLALGAMIGWSWVLMTGLWVQTAG
                     SLGTLIAFAAGGLAIALIGLTYSELASALPRAGGEHVYTHRALGHRWSFVCTWALLFA
                     YVNVCLFESIALPTAVEYLLPGIRMGTLWSVLGADVDVGFVIVGAGSALLVTVVNVLG
                     IKAAARLQSAAIGLILLAGVILVSGAVVFGETDNARPWIATPATGILSVLIMVPAMLV
                     GFDVIPQSAEEINLQPARIGKLLIVSVLLAVAWYGLIAFAVATALSKPELATSSMATG
                     DAATALWGDPIAGRLLVLGGVAGILTSWNAFVIGGSRVMFALAESGSIPAIFARLHPR
                     YRTPYVGVITIGVMCCFAPLFGRTVLVWLIDAGSFAVVVAYLFVPLAFLALRRQEPDL
                     PRPFKVSHPRLVGVGAFVLALGLLTAYLPGSPSALIWPYEWAIVLAWTIVGAVLFLAY
                     GAKHPR"
     gene            complement(12652..14220)
                     /locus_tag="AC16QF_02700"
     CDS             complement(12652..14220)
                     /locus_tag="AC16QF_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR6447491.1"
                     /translation="MDLIGWSWLFLVLYLALMLAFGFIGRRRIKSADDFATARESYGP
                     VFLAFAFAATTASGATFVGFPGIAYDAGLPAVWSVFLYPAGVYLGVLICLKVVSNTGH
                     EFGSRSIPEYLGTRYQSDWMRVLVSLFSLLLFFYLAGQLVSGIVMFEIMLGVSPDWAL
                     AITAAVLMVYVVLGGAHADILTDGVQGLIMVAIAVGLVFLFVSGFGVDGGFTGMIDSL
                     ERQDANLVGWLNPDYVLYHSWWSVAAVLLAHIPLGMLPHIGNKLWALEQPSQRRRFIR
                     LAFTFGLALGMLGLGGLLARAILGDALLQPGESSNTALSMLFVELFPGWLAALLGIGI
                     LAAVMSTADGLVVSSSQIIANDLYRCTIVPRFARELPERTVDRQVLIISRIATVAVMA
                     ICTAMAWALADRNVALIVWIGTGGMMAAFAGPLVVGAVWRGVTKAGAFAGLVGGVTVF
                     SIAHGGLVDPGWFEPGALRDAAAWLLREAPNPWSCAAMGEIVSIALTWGVSKLTQPLP
                     SDHLTRMFGPVGQE"
     gene            complement(14253..15371)
                     /locus_tag="AC16QF_02705"
     CDS             complement(14253..15371)
                     /locus_tag="AC16QF_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HIR6447492.1"
                     /translation="MAERNRAQNPPPPSRSDNGWLKGRGATKNEASRYLARHSVPVDD
                     GWSANEVDEPDGSRVATELYPDRTVTLITRNASPDIPFDRSINPYKGCEHGCVYCFAR
                     PTHAYLDLSPGLDFETKIFFKTNVRERLVDELGKRRYQCRTIAMGTNTDPYQPAERHR
                     RITRSVLETLLEARHPVSIVTKSQLILRDLDLLQELARQQLATVAVSLTTLSNDLKTR
                     LEPRTAGPGARLRVIRELRGAGVPVGVMVAPVIPFVNDHEIEDIVAAATDAGARTANY
                     ILLRLPLEVSGLFEAWLGVHFPDRAERVMNAIRSARGGKTYDATWGKRMRGEGEVAEL
                     IAARFKVALRRNGILGATQPPLRTDLFRPPGGSQLSLI"
     gene            15521..16303
                     /locus_tag="AC16QF_02710"
     CDS             15521..16303
                     /locus_tag="AC16QF_02710"
                     /inference="COORDINATES: protein motif:HMM:NF017434.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease family protein"
                     /protein_id="HIR6447493.1"
                     /translation="MAHRAGPGRALLRAPLLALLMSGCAVTTGSQDNPNAALVSRVQA
                     LEYQVLATQVAVRELAEDQQSGRADLSRQMGDLNTELGRVPEAVGAICASAETAAAAA
                     CDSDQPIQTVVMSGDKMVVGEMERVWLEPPGTTLIARVDTGAHSSSLHAENLVEFERD
                     GEEWVRFDLILDDEIKTLERRVVRYVRVYQQADPEGTRRPVVTMRLRIGDVHDSFEFT
                     LADRSHLDFQLLLGRNFLADMALVDVGKQFVQPPYQPPSTSR"
     gene            16300..17790
                     /locus_tag="AC16QF_02715"
     CDS             16300..17790
                     /locus_tag="AC16QF_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inactive transglutaminase family protein"
                     /protein_id="HIR6447494.1"
                     /translation="MSQKGPFLFLVALLCIAGVGTAAFRHAEYRIPWLPGEQQNVWEI
                     EARIEYMAQGVPTQAFLTLPPNQDGFRVVNETGASSGFGFEVETQRRQRRAHWSKREA
                     NGPQTLFFKLELVRDAEYRVDGPPPAAPPASALDEPYQTAADQIVQAALPVSANARTL
                     AQQLINRMLLVPADQTVSLLRDRFDEATLLANLLVTAGANARTVQVLELEDGRRRQSL
                     KDYVQVWDDGRWYLFNPTVGPVTDLDNLLLWQTDTPSVLEVVGGTRSRVSFSMISQPR
                     SMLALAEPSTGTGFSLHSLPIAEQGMFKLIMLLPVGALMVVLMRIIVGIRTAGTFMPV
                     LIALAFLQTELLPGIVSFVLVIAIGLVIRSYLSHLNLLLVARIATLVILVIGMISVFS
                     IVSYRLGLIGGLTITFFPMIILAWTIERMSIIWEEEGPREVFIQGGGSLLVAVLAFLF
                     MDLTAVRHMAFNFPELHLAVLGLVMLVGRYTGYRLLELRRFIGFKQ"
     gene            17787..18773
                     /locus_tag="AC16QF_02720"
     CDS             17787..18773
                     /locus_tag="AC16QF_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase-like protein"
                     /protein_id="HIR6447495.1"
                     /translation="MIDWITPWELSRRGILGMNRRNIEFIGRYNRREQYPLVDDKLKT
                     KLIAQHAGVQVPDLIDVVRTQHEIETIDDKLADLTEFVIKPAQGSGGKGILVIERHEA
                     GVFTKSSGEQIGILDVKRHLSNVISGLHSLGGRADVAILESLVRVSPLFRNVSYEGVP
                     DIRLIVFQGYPVMGMLRLATLASDGKANLHQGAVGVGLDIGTGKSVAAVQHNRIVRNH
                     PDTGAELTELSVPNWRQLLELAARCFEVTQLGYLGCDIVIDELRGPLLLELNARPGLS
                     IQLANDAGLVPRLRHIENLESFAHNVEDRVDYAMETFTRERRTEQTKLWEGQ"
     gene            18770..19072
                     /locus_tag="AC16QF_02725"
     CDS             18770..19072
                     /locus_tag="AC16QF_02725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6447496.1"
                     /translation="MIIVNAIIESDENSIAAMREGITTMVTATLEEPGCDDYCFSVEL
                     DDPTRIRITERWHSIEALAAHFQTPHMAAFQQVMAANPPKNVTAHCYDAEEIDLPR"
     gene            19079..19714
                     /locus_tag="AC16QF_02730"
     CDS             19079..19714
                     /locus_tag="AC16QF_02730"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6447497.1"
                     /translation="MLTLWGGATSRTLRAHWMLHELGLDYEPRLIGSRTGATQTDAFK
                     QLNPKEKIPVLVDDEFTLTESVAIVTYLGDTYGSGSGLVPEPFTRNRARYNEWASFIQ
                     MELDAHTLYVLRKHRDLAGIYGEAPAAVATAIGGFAKQVAVADEALRGSAHLIGSKFS
                     AADLLLVSCLDWAAAYGLELTPGLDEYRLRIRKRPAYRAAARLNFSISAGA"
     gene            complement(19719..20819)
                     /locus_tag="AC16QF_02735"
     CDS             complement(19719..20819)
                     /locus_tag="AC16QF_02735"
                     /inference="COORDINATES: protein motif:HMM:NF014305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447498.1"
                     /translation="MGQDTDVLLEATMALVPPLLTALDALAHAGRHLHPQSLAHLVPQ
                     IDAFARPLRQGARKFEEIPWPPHLERFRDHISASAGEALAAMDAMSACLEQANPVLAA
                     YQAMGAATRATERLYPVSFMLPPVSRFFLTEAGRDDGALARRLERADPGRDDVGVMHA
                     HNGPGERGGFSLYVPEYYDAGEAWPVVVALHGGSGHGRSFLWNWLKDARSRGVLLVAP
                     TSRDRTWSLAGSDADSASLDAIVERVCEHWRVDERRILLTGMSDGGTFSYLSGLRSDT
                     PFTHLSPISASFHPFILEGVDRDRLTGLPIYLVHGTLDWMFPVDVARTARDALTAAGA
                     ALTYREVDDLSHTYPREENARILDWLMGPSAP"
     gene            20930..21787
                     /locus_tag="AC16QF_02740"
     CDS             20930..21787
                     /locus_tag="AC16QF_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6447499.1"
                     /translation="MPEYDRSAEDVGNVQMLEHVNVTVPDQGLAALFYVTGLGFTRDP
                     YIDFGTYNMWVNLGEQQFHLPQSDAQILRGEIAVVVPNLEDLAKRLKFVSKALKDTRF
                     SWEEADQRVVLSCPWGNRIVAYPAGSFPAMDLGMPYVDMSVPPGTAPGIARFYESVMG
                     CPVSVEPGRVKVAMGYNQKLIYTETEAPLAPYDGHHIAIYVSDFSGPHRFLRERGLIS
                     QESDQHQYRFQKIVDPADDRGEAEPLFELEHEVRSLGHPMYRRPLVNRNPAVNFFTYR
                     RGNEAFTPG"
     gene            21784..22623
                     /locus_tag="AC16QF_02745"
     CDS             21784..22623
                     /locus_tag="AC16QF_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6447500.1"
                     /translation="MSEAAKLARLAEEPYYLPLADEVAVFRSAYEARLPVLLKGPTGC
                     GKTRFVEHMAWRLYRDTQNPGAELEVPLVTISCHEDLTATDMVGRFLLQGDSTIWSDG
                     PLTRAVRTGAICYLDEIVEARKDTTVLIHSLTDHRRLLPIEKTGELVPAHPGFLLVIS
                     YNPGYQSVLKDLKTSTRQRFVSLTFDYPDREREAEIIAHESDVEPAVAERLAALGEKV
                     RNLREHGFEEGVSTRLLIYAAQLIERGIPSRRAVDAAVVQAVSDDATVQQAIADIVDA
                     VLP"
     gene            22620..24608
                     /locus_tag="AC16QF_02750"
     CDS             22620..24608
                     /locus_tag="AC16QF_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HIR6447501.1"
                     /translation="MTALVDVEEPLALFAEGITGRYYHIKAVGEYSGNRMVFDPARGA
                     LTGDALLLPETFEMDDPACYRVLALEQLSIVEFGTLKFSMAEARLRIPALEPPGEPPI
                     TDRASDLERFFNHVSRPHLLSRLFALCERVRLNAQVRDQYPGIRRHQDALHAVLRQSA
                     PRGDTSSVAAILDGLGYRVLGGRNAALESHPLAGALLELMLGVARLDADVFDSARAAH
                     ACYRLCMASAATAEAVAVLEEDLDDAASWLQRDVRLEDWEEGLKQMNGLILAAETAEG
                     EEAVAADVEGLSDGDIRPADVDLRALSNERDVLARRLDMERATIRDAVGRPRPDARSY
                     RYDEWDYLNRSYRTRWCRLFEERLAPDTQQDLDALRHVIRRHRRAVQSQLEHIKPQGY
                     QRVTRVADGDELDFNAVIEARGDIRAGLTPDERVYSRRERVHRDVCAAFLVDLSASTD
                     DPVDPPEAVAAGAVEELANLRDPYEFPVAHDEPAEEPRRIIDVQRESMLVMSAALEQL
                     ADSYGIYGFSGYGRECVEFFVAKEPDEPFTRGTLSAIAGMKPKRSTRMGPAIRHATSK
                     LLRSGHALKVLIILSDGFPQDSDYGPERGDHEYGVQDTAKALREAEEKGVETFCVTVD
                     RSGHDYLKRMCPNARYMVIEETRDLPGALSKVYEALTG"
     gene            complement(24628..25299)
                     /locus_tag="AC16QF_02755"
     CDS             complement(24628..25299)
                     /locus_tag="AC16QF_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HIR6447502.1"
                     /translation="MANYEVSSVRAAAAPAIEINKVLRNTYMLLGLTLAFSAVVATAA
                     MIADAPYLGFLPTLVGFFGLLFIVHKTANSPWGLFWTFAFTGFLGYGLGPILNAYLTL
                     ANGDVLIASALGTTALAFVGLSAFALVTRKDFSFLSGFLVVGFFVLMGAVLLAWLFDL
                     SGFHLAISCGFVLFACAAILWQTSAIIHGGETNYILATITLYVQIYNLFISLLHIFGA
                     FGSDD"
BASE COUNT         4515 a         8426 c         8141 g         4254 t
ORIGIN      
        1 tcggccgttt ttcagcggtc tgatgtctac ccatcccccc ctcgaagacc ggatccgcgc
       61 catcgaaccg cgctgggacg gtcggctcct ggaacgtggc caaccgtccc cgacgcgccc
      121 tgccggcgcc gcgggatttg cgggtaccgg cgcagcgggc attcaagcac cggggggcat
      181 ctcggtgggg gtccccgcgg agagcatcgc ggacgaggtc ggccgcctgg accagacgag
      241 cctggacaac gcggtgggca tcatcgcgag catcgatccg aagctgcgcg aagctgctca
      301 cgacccctgg tccggtcgcg ggctgatcta cgcgaccctc acccacgccg atggctatgc
      361 gcgcgaccga cagctcgcct atctcgagga aaacgcggaa cccggcgtgc ccgaacacgt
      421 ctttcgcctg gttcccgcgg tcaggcatct cgacgagccc cgcaagctcg cgctggtgga
      481 aatggcggtg ccggcattga aagagctgtc cgacccccag ttcaggcggt tcatcgacaa
      541 tgtggttgcg atgatcaggt ccgatcgcca aatcgacctg ttcgagtggg tcatgcacag
      601 gctgctgatc aaggagctgc gtccgcactt cgacggcccg gagcggatca gggcccgcta
      661 ccgctccccc gaccaggtgg ccgatgcagc gctgacggtg ctttccgcgc tggcacgttc
      721 cggtcatggg gatgtcgggg ctcaggccaa ggccctggcg accggcgccg agctgctcga
      781 ccttgcgcct cgaatggaca cagaagacga ccccaacttc gtccgtctcg gtcaggccct
      841 ttggcagctt cgccatctaa agccgctggt gaagccgcgc gtgctgaaag cctgcgctgc
      901 cgtagcgctt gccgacggcc aggtaagcgg ccgagagggc gccctgctgc agggcatcgc
      961 agcgacgctg gattgcccgt taccgccctc gatctacgct ccgaagtcct gagagggagc
     1021 aagcgccccc gcttgcgcgt ccgaccttgc tctatagaat gacgctttcg atccgcactg
     1081 ggccccatga ccgaggccac tctcaactac ggcgacgtca cccagctcgc ggcactggac
     1141 ctgggctcaa acagctttca tctcatcgtc gctcaggaaa ccaacgggcg gctgcagatc
     1201 atcgacaaga tcaaggagat ggtcaggctc gcaaacggcc ttgacgagaa gaacgagctg
     1261 accgggcccg ccacggatac agcgctcgcc tgcctcgagc ggttcggcca gcggctgaac
     1321 agcctgcagc gccgcaacgt tcgcgtcgtg gggaccaata ccttgaggcg cgcgcgcaac
     1381 gcggccgcct tcatcgaacg ggccgagcag gctctaggcc acccggtgga gatcatctcc
     1441 gggcgcgagg aggcccgcct gatctatctg ggcgtatctc actccatcga ggacaactac
     1501 gaacgccgcc tggtcatcga catcggcggc ggcagcacgg agctcatcct cggccgccat
     1561 ttcgaggcgg agaaaaccga gagcctgcac atgggatgcg tggggatctc gcgacagttc
     1621 ttcggcgacg gacgtatccg ggccggacag tttcgagcgg ccgtcaatca cgccagaaaa
     1681 gagctcgttc cggtggccgg aacctacggc agaaagtccc gggacacggt gatcggcgcc
     1741 tcgggtacca tcctggccgc tcaggccgtg cttgcagaga tggacgggag cagggacatt
     1801 tcgctcgatg gtctggaacg cgtgcgcgag gccctgatcg atgccggcca cgtggacaag
     1861 ctggctttgc ccggtctgcc ccccgagcgg gcgccagtat tcccgggagg ccttgccgtg
     1921 ctcatcgccg ccttcaaaca gctcgacatc gcgagcatgg aggccaccag cggcgctctc
     1981 cgcgaagggc tgctctacga tctgctgggt cgggtgcacc accaggacgt gcgggagaac
     2041 agcgtcgcgg acctgtgcat gcgctatcac gtcgaccaga cacacgcgcg ccgggtgagg
     2101 gagatggcgc tggccctcct ggcccaggta gccctcgaat gggatctcac cgagccgaga
     2161 catcagctcc tgctggggtg ggcggccgat ctgcatgaga tcggcatgga catcgcgcac
     2221 agccagtacc acaggcacag cggctacctg ctcgccaaca tggaccttcc gggcttctcc
     2281 aaaggcgacc agctggagct ggccgcgctg gtccgagccc accggcgcaa gttccagcag
     2341 gaagacggcg attatccccc ccatgccctg aggctagcgg tgctgctgag actggccgtg
     2401 ctcctgcacc gcaaccgcgc caacacggcg ctccctcacg tcgcgctgcg agcagacgga
     2461 gacgcgctgc tgctgaagtt tccccgcggc tggctcaaac gccacccgct cacccgcctg
     2521 gatctggacc aggaagccga ctacctgtcg gcggtcggag tcggtctgcg catcgacgag
     2581 aacgaaaacg ccgcgcgatc agtcgccggc taactcttcg agcagccctt cctgagcgct
     2641 tcgacgctcg ccctccggag ggacaagccg ccggtacttc ccgtccgctg cgagcatcca
     2701 agactggcag ttgtcgccga gatacatccc gagctccctg tagatccggt cacgcaggga
     2761 ggcgtcctca atgggaaagc aggcctcaac ccggtcgaag aagttacggt tcatccagtc
     2821 cgcgctggac agatagaccc tcggatcgcc ttcgttctca aagtagaaca ccctggtgtg
     2881 ctctagaaac cgcccgatga tcgagcgaac ggtgacgttc tcggacacgc cctcgatacc
     2941 cgggcgcagt ccgcatacgc cgcggaccac gaggtcgatc ttcaccccgg cctgactcgc
     3001 ggcgtacagc gcctgtatga cctgcggctc gatgagcgag ttcatcttgg ccagtatccg
     3061 cgccggtcgc cccgcgcggg cgttctcggc ttcgcgctcg atgaagacga gcatggacga
     3121 atgcatcgtg aacggtgcct gcagcagctt cttcagcccg ctggtcttgc ccatggacgt
     3181 gagctgctga aagagctgct gaacgtcgtc gccgatgtcg gggtcgcagg tgaacaggcc
     3241 gtagtcggtg taaagccgcg tggtgcgcgt gtggtagttg ccggtgccca gatgcacata
     3301 gcggcgcagc ggacggcctt cccgacggat gaccatgctc atcttcgcgt gggtcttgtg
     3361 ccccaccacg ccgtaaacca cctgtgcgcc cgcgcgctga aggtagttgg cgagctcgat
     3421 gttcgcctcc tcgtcgaaac gcgcacgcaa ctcgatgacc accagcactt ccttgccgtt
     3481 ggtcgccgcg tcccgcagcg cttccaccac cgccgactcc gtccccgtgc gatagagcgt
     3541 ctgacggatc gaaagcacct ggggatcggc ggccgcctgt cgcagaaagt cgatgaaagg
     3601 cgcgaacgac tcgaaagggt gatgcagcag cacgtctccc tgacggatga cctcgaacat
     3661 gtcgttggcg ttacgcagcc gatcgggaat acccggcgtg aaccccggaa acttcagctc
     3721 cgggcgatcg acgaggtccg gaacggtcgc caggcgcatc aggttgacgg ggccactgca
     3781 gcgatagacg tcttcgggct tgaggtggaa ctggccggtg agaaaagctt ccagaggatc
     3841 gggacagtcc cgggcgactt cgaggcgaac ggcatccccg aaccggcgcg aggaaagctc
     3901 gccttcgagc gcccgcaaca ggtcgtcgac ttcttccatg tcgacgaaaa gatcgctgtt
     3961 gcgcgtcacg cgaaactgaa aacagcccgt gctcttcatg cccgggaaca ggtcaccgac
     4021 gtgcacctcg atgatcgacg agagcagaac gaactcgttc ggcgtggcgg cgcaggactc
     4081 cggcaggcgc accaccctgg agagcgcgcg aggtgcctga acgatggctt tgccgctgcg
     4141 gcggccgaag gcgtcttcgc cttcgagggt gacgatgaag gtgaggctct tgttcagcgg
     4201 ctgggggaag gggtgcgcgg ggtcgagcgc gatggggctc atgatcggcg cgagctcccg
     4261 cttgaagtac cgcttgagcc actgcctttg cttgctgctc cactcgtccg gatccagaat
     4321 tcggatgccc tgatcgtcga gactcggaag cagatcttcg ttgagaaccc ggtactgttc
     4381 ttcaacaagc tctcggacgg tctcgaagat gcgccgcagc tgctcgctgg gagacaggtt
     4441 gtccggtccc cgctgtgtgg cgcgatacgc ctcctgctgc ttgagaccgg cgacccggat
     4501 ctcgaaaaac tcgtcgagca ccgagctcga tatgcacaga tagcgcaagc gttccaagag
     4561 cggcacgcgc tcttcctggg cctgggcgag cacgcggcga tgaaactcta gcagcgagag
     4621 ctcacgattg atgtagagct ccggattcgc gagcccggcg aagtccgtgg aagggtttgc
     4681 gctcaactga gactctgact aacaattgac accgcatttt accatgcgcg ccggaggcca
     4741 atgttaaagc ttcgtgttgc cgaagggggg ccgcctcctt gagttcccgc gaccgcgtct
     4801 accgcgaatc aaaaagacag atcgtcgact tcgccttcga cgaagccgtg gccgacgtgt
     4861 ttcccgacat gattcgccgc tccgtccccg gctacgaaac cgtgatcccg atcacgggaa
     4921 tgctggcggc caggcatctc gagcacatgc ccgacgagcg ccgtctggct tacgacctgg
     4981 gttgctcgct gggcgccacc accaccgccg tcctggacca actggacgaa cggcccgctc
     5041 gcgtcattgc cgtggacaac gctgcggcca tgatcgcccg agcggtcgac gccatccgtg
     5101 acccgcgggc gcgcttcgag gttgccgacg ttaccatgct ggagttcgaa cctgccggcg
     5161 tcgtgctgat gaacttcgtg ctccagttca tcgaccccac gcaacgggtg ccactcctga
     5221 aacgaatcag gcaagcgctc gaaccagacg gcttgctcat cgtctccgag aagctgcgct
     5281 tcgaagatcc ttacacccag gccctgttcg agcgggccca cctcgactac aagcgtgcca
     5341 acggatactc tgagctcgag atcagccaga aacggtcagc actggaaaag gtgatgattg
     5401 tcgacaccga agcagagcat cgcagccgct tcgaagccgc cggattcgca accaccaaca
     5461 gatggttcca gtgcctgaac tgggtttcct acctcgttgc cccatgatct ttgagcccgt
     5521 cctggatctc atacccgaat ggcgccggct cggcccggac ttaaacgcgc gcctgtcgac
     5581 gaaggggcac ggcgactacc cgcgctggga agccgcgctc gcagcgctgc ccgacctcca
     5641 ggcagagcag gccgccctca gcgatcgggt aacggtgagc gggaacgcct ccgaagacca
     5701 gcggcgcgac ctgcgggaat cgctgatggc cctgcatccg tggcgcaagg gacccttcag
     5761 cctgttcgaa gtggacatcg actgcgagtg gcgctcggac tggaagtggc tgcgcgtgct
     5821 cccccacctg gattcccttt ccgggcgccg cgtcctcgac gtgggatgcg gcaacggcta
     5881 ctacggctgg cgcatgctgg aagccggcgc caccgcggtt ttcggcgtgg atcccacgct
     5941 ggttttctgc atgcagcatt tggcagtcag ccgctacctg tccggcgcgg ccaactgggt
     6001 gctgcccctg aggttcgagc agcttcctcc gcatccgttc gataccgttt tcagcatggg
     6061 cgtgatttat caccgacggg atcccgccga gcacgcccgc cggcttgcgg agttcacgag
     6121 gcccggtggc caggtcgtgg tggaatcact cgtggtcgag cacggccgat ctttgaagcc
     6181 cgtaggccgc tacgcgcgaa tgcgcaacgt tcacgtggtg cccacgacga aacggctcgc
     6241 gcgctggctc aaggacgcgg gcttcgtcga tccggagctc gtcgacctga cacctaccac
     6301 ggtcgccgag cagcgcgcga cgacgtggat gcgattcgaa tccctcgagg acgctctaga
     6361 tcccaagatg cggaaccgaa ccatcgaagg tcatcccgcc cccgtccggg ccgtcatggt
     6421 cgcgcgcaag ccggtctgat tcgcgccggc gggcgtggaa atgcgctttg aaaccgcgta
     6481 cggtctcaag acggcaaagc cgcagaacac aaccatgatg ctcgggatca ttcccatcag
     6541 ttttcgcttc cgcggcaaga tcatgcggct ggaggacatg tatcactgct ttcgggagca
     6601 tcccggaacc gggctgcatc cgacgcagat atcccgctat acgggcctgg gtctggccga
     6661 ggtgaacgcc agactggaag cgactcccga gctcttcgtg cgccttccga aacgccccga
     6721 cggcataacg cgctaccggc tgaccagtgc caccacggca aaggcgcccg aggacgttga
     6781 gaagttcctc cgccaggcgg ccaggcgcga aagcctgatg ctttacgccg tgggaagcat
     6841 ggtgctgttg attctgctca tcgtcgtcat cctcgtcggg cctgccgtct agatgcccga
     6901 actgctcgac gataagcagc gcgcgcttcg cgagcacgcc gcggccttcg cccgtgagca
     6961 gctggaaccc agacgcagca tggacaacaa gcaggcgcgt gaggaaatcg tccgcgctgc
     7021 caaagccgcc ggtttctacg ccatgaccca gcccgcggag ttcggcggca acccggcgtc
     7081 ggttctggaa ctcacgatcg tcagagacga gttggcggcg agcaacgcgc cccacgccga
     7141 tgcagtgttc gggccgggtc cgggggtgct ggccggagtg ggagagcccc tggctagcga
     7201 ctaccttggg cccgtgctgg cgggcaccaa gcaaggcgcg ttcggtttca ccgagcccga
     7261 tgacgccccc ggaccaacca gcgggcggct ggaatcagac gagctgatcg tcagcgggca
     7321 gaagtcgtac gtcaccggcg gcgagcaggc cgacttcatc aacaccctcg tgcagatcga
     7381 gggccacggc cccgcgctgg tcgtgatcga tacccatgcc gccggggtga tccgcgagcg
     7441 aaccttctcg tcgctagacg gcagccgtca cgccgccttt cgattcgaag aagtgcgcgt
     7501 gccccgctcg cgcgtgatcg gcaagcccgg cgaggggctg ccgcgagcca tgcgccagat
     7561 cggcgatacg cgcctggcca tcgccgccca gtgcgtgggg gccatgcgat tcgttctcga
     7621 actgctcacg acgcacctca aggcgcccga ccgctccggc cgggcgcgcg gcagcaagga
     7681 aggcgtgcgg cttcgctacg ccgatctgcg catcaaagcg tatgccgcgc gcagcatggt
     7741 ttaccggacg gccaggctcg cggactccgg gtccaacgtg atcaacgagg ggatcgcctg
     7801 caaagtgttt gcaacggaaa ccgccggcga gatcgtcgac accgggatcc agctggttgg
     7861 cgggagcgcg ctggtggagt cccacccgct ggccgagctc taccgacggc tgcgcgcgct
     7921 gcggctggca gagggtggca gcgacgtgct gcgactgaac ctggcgcgag ggcgcctgga
     7981 cctggacaaa ggccggatct gaaaatgccc gcccgcgggc acagcttcag ccgggttcct
     8041 cggcttcccc cttgccgcca tattgctcgc gattattttc acgggcctga ggggacttct
     8101 tcagcagcac gtagcaggcg ccggtaccgc cctggttgtt ctgggcgctg tgataggcga
     8161 tcacctcggg cacctgagca agccaggcgg ccacgtagct tttgatacgc gccggggtct
     8221 cgctctgctc gccgcggccg tgggcgatga gcaccatgcg ccagcccttc gccatggcga
     8281 gattgagaaa ccggaacacg gcggctcggg cttccctcac cgtttgccgg tgcagatcca
     8341 cagaaccctc caccggatac tttcccgcct tcagccgcgc gaagacggcg ttctgcacac
     8401 cgtccttctt ccactcgaca atctcccgcg gctgccgctg gggcacctcg ccaagggtca
     8461 ggtaattcgg gtccgcggcc tcgcggtccc ggttgccgag ggcgaattcg cggcgttcga
     8521 gctgcgcgga ggtcgcctcg accggccctc gctcgggttg cgcgcgctgc ttgcgctgca
     8581 gcggcgcaac gtcgctcatg gcctcgcgaa agacgtcctc gtcgcccatg attcggctac
     8641 agcggcactc ccggatactg cgcagaccgg gtatccggga tcggccggtt gtcggcggtg
     8701 tggtacgtgg tcaccacgga aagcttcacc tcgtccgtga gattcttgcc tgccgcgtgg
     8761 aacaaccggc agtgaaaaaa cagcacgtcg cctgcttcaa gcgggaccgt ctgggcatgc
     8821 tcaacgagct cggcgttctc ggcgagctcg gggcgcagga aaagatcccg gtcgagccgg
     8881 ccccgatcga tgttcatggc gtgactgccc ggaatgacct tgagcgcgcc gttttcctcc
     8941 gtctcttcgc ccagcgcgag ccagacggag acgagctcgg ggcggtcgaa cgaccagtac
     9001 cggatgtcct gatgccacag cgtcgcgctg ctgtagccgg gatgcttggt catcacgcag
     9061 ttgtgatgac attgcgacat catcacgtcc gcgcccccga aaagaacgcg gagaacggcc
     9121 cccacggagc ttcctgtggc ccagtccctg aaaacagaat ctcgagcgta ggcatgcagg
     9181 agacggcggc acgtatcgcc cccatccgaa gcgcggctcg caggggatcc gggatacccc
     9241 acgtcagctt cgaactctgc gggccccagc aggggctgga gataggaaga ggcgcgatcg
     9301 cgaatccgcc tcaccagccc cgggtcgcag cagccgcgca ccacgacgaa gccgtcgcgc
     9361 tcaaaagcgc gcggctcgag gcggtcgatc tcgctgctgc cggattccaa ggtacgctca
     9421 acgcatctgg gcgcggatgg ccttgagcat cttctcgccg tagggcggac gcaggccggc
     9481 cagctcggcc acgttgacct tgctctgggt gaagatcgac ttggcgtggc tgaatgtctt
     9541 gaagccgtcg tgcccatggt aggcgcccat gcccgacggc cccaccccgc cgaacggcag
     9601 atcctcctga gccacgtgca taaccacgtc gttcaccgtc accccgcccg aggtggtgtg
     9661 cgaaaggacc tttccttcct cttcgcggtc gtcgccgaag tagtacagtc ccagcggccg
     9721 gggatggtgg ttgatgtagt cgatggtgtc ggcaacgttg gcgtaccctt tgaccggaag
     9781 aatcggcccg aagatctcgt cctgcatgac ctgcagcgac tcgtccgggt cgacgatcag
     9841 cgtcggcggg atcttgtgat gcggttgctg acggaagtcc tcgttggccg ggttgacctc
     9901 tactaccttg gcgcctttct cccgagcctc ctccacgtag ccctggaggc ggtcgtagtg
     9961 acgctgattg atcaccgacg tgtagtccgg attgtccagc agcgtcgggt acatcttctc
    10021 gacggaacgc gccgaggact cgacgaactc atcgacccgc tcttcgggca cgaagacgta
    10081 atccggagcg aggcagatct gtcccgcgtt catcatcttg ccggtcatga tcgcatccgt
    10141 ggttttctgg atgtcggcag agcgcccgac caccaccggc gatttgcccc cgagctccag
    10201 ggtcacgggc accaggtttt ccgacgccgc ccgcatcacg tgcctggcga tggatgtggc
    10261 tcccgtgaac agaagatgat cgaacggcag ccccgagaaa tccgcgccca cctgagggcc
    10321 accggtgacc accgctatct ccagctcgtc gaaggccgtc gggaagatct ctgccataac
    10381 ctccgaggtg gccggcgtgt actcggacgg cttgatcatg caccggttgc cggcgccgag
    10441 gatgccggcc aggggcgtaa acgtcaggtt gaccggaaaa ttccacgggc tgatgacgcc
    10501 caccacgccg aggggctggt actccaccct cgccttcgcg ccgagcacgt tcagcgggaa
    10561 cggtccaacc ttgcggcgct ccgggcgcat ccagcgggcc aggtgcttct gagcatgccg
    10621 cagcggtccg atggagccgg caacgtcggt aagcatcgac tgatgctcgc tgcgatggcc
    10681 gaagtccgcc gtcatggcct ctaccagccg cttctcgtgc gtcttgagga gacccacagc
    10741 ccgctcgagg cgatccacgc gcttggccaa cgaaacggtt ccttccccaa ggtaatcctc
    10801 tcgctggcgc tcgagaagcg ccagcatctg ctcaagggtc atgtcctgca tgtctgctcc
    10861 cagttcgtaa tctcggcgtt tcgaggaccg gccatcatac tcgccttcaa tctcgatctc
    10921 gacgcgcctc atcggcccgt ctgcagtagg cggcgctcat ccctcaaggc agcgatcggt
    10981 cgtaaatccc gtcaccattg accgtcacct tcaggcgtcg gttccctgcc cagtaacggg
    11041 cctctatgta atggtgctcc tcccactgga gctcgacgtt ggtccagacg tactgaggct
    11101 cccggcccgt cggctcgcga cgcttgcgca ggcacccgtc gacatggagt tccagggcgc
    11161 cgtcgtccgc caggcggatc tcgaactcct tgccgcgatg gctcaatgcg acgacgtctg
    11221 gcaaaccagg ctccaggtgt tgaaatcgaa aggtcgaggg aggcggcaat taccgctaag
    11281 cttgcgggct cgaattctgc ccgcaggcgc cacgcgatgc cagcaagccc tgaccaaccc
    11341 cgctttgccc gagtgctgaa gagccgggaa gtcatcaccc tggcgctggg cgccatgatc
    11401 ggctggagct gggttctgat gaccgggctc tgggtgcaga cagccggcag cctgggcacg
    11461 ctcatcgcct tcgccgcggg cggtctggcc atcgcgctga tcgggctgac ctacagcgag
    11521 ctcgcctccg ccttgccgcg cgcgggcggc gagcacgtct acacccatcg cgcgctggga
    11581 caccgctggt cgttcgtttg cacctgggcc ctcctgtttg cctacgtgaa cgtgtgcctg
    11641 ttcgaatcca tcgctttgcc cacggcggtg gagtatctgc tgcccggtat tcgcatgggt
    11701 accctctgga gcgttctcgg tgcggacgtt gacgtcggct tcgtgatcgt gggcgcgggc
    11761 agcgcgcttc tggtcaccgt ggtcaacgtg ctgggcatca aggccgccgc gcggctgcag
    11821 agcgcggcca tcgggctgat cctgctggcc ggcgtgattc tcgtcagcgg tgcggtggtc
    11881 ttcggggaga ccgacaatgc caggccttgg atcgccacgc cggccaccgg gattctcagc
    11941 gtgctgatca tggtgcccgc catgctggtg ggctttgacg tgattcccca gtccgccgag
    12001 gagatcaacc tccaacccgc ccgcatcggc aagctgctca tcgtatccgt gctcctcgca
    12061 gtagcctggt acgggctgat cgccttcgcc gtcgccacgg cgctaagcaa acccgagctg
    12121 gccacgagct ccatggccac gggcgacgcc gccaccgctc tctggggtga ccccatcgcc
    12181 gggcgcctcc tcgttctcgg cggcgtggcc ggcatcctca ccagctggaa cgccttcgtg
    12241 atcgggggca gccgcgtgat gttcgcgctg gcggagtcag gctccatccc ggccatattc
    12301 gcccggctgc atccccgcta ccggacgccc tacgtcgggg tcatcaccat cggcgtgatg
    12361 tgctgctttg cgcctctatt cggacgcacc gtgctggtct ggctcatcga tgcggggagc
    12421 ttcgccgtgg tggtggctta cctgttcgtg cccctggcct ttctggccct gcgacgccag
    12481 gagcccgacc tgccccggcc gttcaaggtc tcccatccgc gcctggtggg tgtcggcgcc
    12541 ttcgtgctcg ccctcggcct gctcaccgcc tacctgcccg gcagcccgtc tgcgctcatc
    12601 tggccctacg agtgggccat cgtgctcgcc tggaccattg tcggcgcggt gctattcctg
    12661 gcctacgggg ccaaacatcc gcgttagatg atctgacggc aggggttggg tgagcttgga
    12721 cacgccccag gtcagcgcga tggacacgat ctcccccatg gccgcgcagg accaggggtt
    12781 gggcgcttcc cggagaagcc aggcggcggc atcgcgcagc gccccgggct cgaaccagcc
    12841 ggggtcgacg aggccgccgt gggcaatgga gaaaacggtt accccgccca ccagaccggc
    12901 gaacgcgccc gccttggtca cgccgcgcca gacggcccca accaccagcg ggcccgcgaa
    12961 tgccgccatc atgccccccg ttccgatcca cacgatcagg gcaacgtttc tgtcggccag
    13021 ggcccaggcc atggcggtgc agatggccat taccgccacg gtcgctatgc ggctgatgat
    13081 gagaacctgc ctgtccacag tgcgctcggg cagctcgcgc gcaaaccggg gaacgatggt
    13141 gcagcggtaa aggtcgttgg cgatgatctg cgacgacgaa accaccaggc cgtcggccgt
    13201 ggacatcacc gccgcgagga tgccgatacc cagcagggcg gcgagccatc ccggaaaaag
    13261 ctccacgaag agcatggaca gcgccgtgtt gctcgactcg ccgggctgca gcagggcgtc
    13321 tcccagaatt gcccgcgcga gcaggccgcc cagccccagc atccccagcg cgaggccgaa
    13381 ggtgaatgcc agccggatga atcggcggcg ctggctgggc tgctccagcg cccacagctt
    13441 gttgccgata tgcggcagca tgcccaaggg gatgtgggcg agcagcaccg cagcgacaga
    13501 ccaccaggag tgatagagga cgtagtccgg gttcagccag cccaccaggt tcgcatcctg
    13561 gcgctcgagg ctgtcaatca tgcccgtgaa gccgccgtcg actccgaagc ccgagacgaa
    13621 gaggaagacg agccccaccg cgatggccac catgatcagt ccctgcaccc cgtccgtgag
    13681 gatgtcggca tgggccccgc ccagcacgac gtagaccatc agcaccgccg cggtgatcgc
    13741 cagcgcccag tccggggaga cgccgagcat gatctcgaac atcacgatcc cggagaccag
    13801 ctgacctgcc aggtagaaga agagcagcag ggaaaataga gagaccagaa cccgcatcca
    13861 gtccgactgg tatcgggtgc cgaggtactc cggaatgctt ctgctgccga actcgtgccc
    13921 ggtgttgctg acgaccttga ggcagatcag gaccccgagg tagacgcctg cggggtaaag
    13981 aaagacggac catacggcag gcagccccgc gtcataggcg atacccggga agccgacgaa
    14041 ggtcgcgccg cttgccgtgg tggccgcaaa ggcgaaagcg agaaacacgg gcccgtagga
    14101 ttcccgcgcg gtcgcgaagt cgtcggcgct tttgatccgc cttctgccga tgaagccgaa
    14161 ggcgagcatc agcgccaggt agagcaccag aaataaccat gaccagccga tcagatccat
    14221 gctcaccctc cccgccgagc ggttaaagct tatcagatca ggctcagctg gctgcctccg
    14281 gggggtcgga agagatctgt gcgaagaggc ggctgggtgg cgccgagaat gccattgcgc
    14341 ctgagcgcga ccttgaagcg cgcggcgatg agctcggcaa cttctccctc gccccgcatt
    14401 cgcttgcccc acgtggcgtc gtaggtcttg ccgcctcggg ccgagcgtat cgcgttcatg
    14461 acacgctcgg cgcgatccgg aaagtgcacg cccagccagg cttcgaagag gccgctcacc
    14521 tccagcggca ggcgcagcag gatgtaattc gccgtgcgag caccggcatc ggtagccgcg
    14581 gccacgatgt cctcgatctc gtgatcgtta acaaagggga tgacgggcgc caccatgacc
    14641 ccgacgggaa cgcctgcgcc ccgcagctcc ctgatgaccc tgagcctggc cccaggtccc
    14701 gccgtgcgcg gctcaaggcg ggtcttcaga tcgttgctca gcgtcgtgag gctcaccgcc
    14761 acggttgcca gctgttggcg agccagttcc tgaaggaggt cgaggtcgcg cagtatgagc
    14821 tggctcttgg tcacgatgct caccggatgc cgggcttcca gcagcgtctc cagcacgctc
    14881 cgggtgatcc gccggtgccg ctcggcgggc tggtacggat ccgtgttggt ccccatggcg
    14941 atcgtgcggc actggtagcg gcgctttccc agctcgtcga cgagccgctc gcggacgttc
    15001 gtcttgaaga agatcttcgt ctcgaaatcc agccccggcg agagatccag gtaggcgtga
    15061 gtgggccggg cgaagcaata gacgcagccg tgctcacagc ccttgtaggg attgatggaa
    15121 cggtcgaagg ggatgtccgg cgaggcgttg cgggtgatga gcgttactgt tctgtccgga
    15181 tagagctccg tggccaccct ggacccgtct ggctcatcga cttcgttggc cgaccagccg
    15241 tcgtccacgg gcacgctgtg ccgggcgaga tagcggctcg cctcgttttt ggtagcgccg
    15301 cggcccttca accagccgtt gtccgaccgg ctgggcggcg gcggattctg cgcgcgattt
    15361 ctctctgcca ttgaagggcc ttccaacaga caggcagaat aactgtatat atgtacagta
    15421 tcaagcgtcg gataccgaca gtagacttcg acgccgaagc ccaaactgac tgcagatgaa
    15481 ccgaccgaga acggcctttg aagacgttcg gcgcggcgct atggctcacc gcgccggtcc
    15541 gggccgcgcc ctgctgcggg cgccgctgct ggcgctgctg atgagcggat gcgcagtcac
    15601 caccggcagc caggataatc ccaacgcggc gctggtcagc cgcgtgcagg cgctggagta
    15661 ccaggtgctg gcgacccagg tcgcggtcag ggagctggcc gaggatcagc agtcgggccg
    15721 ggccgatctc tccaggcaga tgggcgacct caacactgag cttggccgcg tgcccgaggc
    15781 cgtcggcgcg atctgcgcca gcgcggagac cgccgcggcg gcggcctgcg attccgacca
    15841 gcccatccag accgtggtca tgagcggcga caagatggtg gtcggcgaga tggagcgggt
    15901 ttggctggag ccgcccggca cgaccctcat cgcccgtgtc gacaccggtg cccattccag
    15961 ctccctgcat gcagagaacc tggtggaatt cgagcgcgac ggcgaggaat gggtgcgctt
    16021 cgacctgatt ctggacgacg agatcaagac tctggaacgc cgggtggtcc gctacgttcg
    16081 cgtctatcag caggccgatc ccgaaggcac ccgccgaccg gtggtcacca tgcgcttgcg
    16141 tatcggcgac gtgcacgact ccttcgagtt caccctggcg gaccggtcgc acctagattt
    16201 tcagctgctg ctgggacgta acttcctcgc cgacatggcg ctggtggacg tcggcaagca
    16261 gttcgtccag ccgccctacc agcccccgtc aacctccaga tgagccaaaa ggggccattt
    16321 ctcttcctgg tggccctcct gtgcatcgcc ggcgtcggca cggccgcgtt ccggcacgcc
    16381 gagtaccgga taccctggct gcctggcgag cagcagaacg tctgggagat tgaggcgcgc
    16441 atcgagtaca tggcccaggg cgtcccgacc caggcctttc tgacattgcc ccccaatcag
    16501 gacggcttca gggtggtcaa cgaaaccggc gcatcgtcag gcttcggctt cgaggtagag
    16561 acccagcgcc ggcagcgcag ggcacactgg tccaagcggg aggcgaacgg gccgcaaacc
    16621 cttttcttca agctggagct cgtgcgcgat gcggaatacc gggtggacgg ccccccgccg
    16681 gcagcgccgc cggccagcgc cctggacgag ccgtaccaga cggcggccga tcagatcgtc
    16741 caggccgccc tgccagtcag cgccaacgcg cgcaccctgg cacaacagct gatcaaccgc
    16801 atgctgctgg tgcccgccga ccagaccgtc agccttctgc gcgaccgctt cgacgaggcg
    16861 accctgctgg ccaacctgct ggtcaccgcc ggcgcgaacg cgcggacggt gcaggtgctg
    16921 gagctcgagg atggacgacg ccgacagagc ctcaaagact acgttcaggt atgggacgac
    16981 ggccgctggt acctcttcaa ccccacggta gggccggtga cggacctgga caatctcctg
    17041 ctatggcaga ccgacacgcc ctcggtgctg gaggtggttg gcgggacgcg aagccgcgtg
    17101 tctttctcca tgatcagcca gccccgctcc atgctggccc tggccgagcc ttccacaggg
    17161 accggcttct cgctgcacag cctgcccatc gccgagcagg gcatgttcaa gctgatcatg
    17221 ctgctgccgg tgggcgccct gatggttgtg ctcatgcgga tcatcgtcgg catccgcacg
    17281 gcgggcacct tcatgccggt gctgatcgcc ctggctttcc tgcagacgga gctgctgccg
    17341 ggcatcgtca gcttcgttct ggtcatcgcc atcggcctgg tcatccgatc ctacctgtcc
    17401 catttgaacc tgctgctggt ggcgcgcatc gccacgctgg tgattctggt gatcggcatg
    17461 atcagcgtgt tcagcatcgt cagttaccgc ctcggtctca tcggcgggct gaccatcacc
    17521 ttcttcccca tgatcatcct ggcctggacc atcgaacgga tgtccatcat ctgggaggaa
    17581 gaggggccgc gggaggtgtt cattcagggc ggtgggagcc tcctggtggc cgtgctcgcc
    17641 ttcctgttca tggatctcac cgccgtccgg cacatggcct tcaatttccc cgaactgcac
    17701 ctggccgtgc tcgggctcgt catgctggtc ggccgctaca cgggttaccg cctgctcgag
    17761 ctgcgcaggt tcatcggttt caagcagtga ttgactggat cacaccctgg gagctgtcgc
    17821 gacgcggcat cctgggcatg aaccgacgca acatcgagtt catcgggcgc tacaaccgcc
    17881 gcgagcagta cccactggtc gacgacaagc tcaagaccaa gctcatcgcc cagcacgcgg
    17941 gcgtccaggt gcccgatctc atcgacgtgg tccggaccca gcacgagatc gaaaccatcg
    18001 acgacaagct cgcagacctg acggagttcg tgatcaagcc ggcccagggc agcggcggca
    18061 agggcattct cgtgatagag cgccacgagg ccggggtctt caccaagagc agcggcgagc
    18121 agatcggcat tctggacgtc aaacgccacc tctccaacgt catcagcggg ctgcactccc
    18181 tgggcgggcg cgccgatgtg gctattctgg aatctctggt gcgcgtcagc ccgctgttcc
    18241 ggaacgtgag ctacgagggt gttccagaca tccggctgat cgtctttcag ggctatccgg
    18301 tcatgggcat gctgcgcctg gccactctgg cctccgacgg caaggcgaat ctgcatcagg
    18361 gtgcggtagg ggtcgggctc gacatcggca cgggcaagag cgtggccgcc gtgcagcaca
    18421 accggatcgt tcgcaatcac cccgatacag gggccgagct gaccgagctg agcgttccga
    18481 actggcgcca gcttctggag ctggctgccc gatgcttcga ggtgacacag ctgggctacc
    18541 taggctgcga catcgtcatc gacgagcttc gcgggccgct gctgctggag ctgaacgctc
    18601 ggccggggct gagcatccag ctggccaacg acgcggggct ggtgccgcgc ctgcgacaca
    18661 tagagaatct cgagtctttc gcgcacaacg tcgaggatcg cgtcgactac gccatggaga
    18721 ccttcactcg agagcgcaga acggaacaga cgaaactctg ggagggacaa tgatcatcgt
    18781 caacgccatc atcgagtcgg acgaaaacag catcgccgcc atgcgcgaag gcatcaccac
    18841 catggtgacg gccacgctcg aggagcccgg atgcgacgac tattgcttca gcgtggagct
    18901 cgacgatccg accaggatcc gaatcaccga gcgctggcac agcatcgaag cgctggccgc
    18961 ccattttcag acgccccaca tggcggcgtt tcagcaggtg atggccgcca acccgccgaa
    19021 gaacgtcacc gcccactgct acgacgccga agaaatcgac ctgccgcgct gagccgtcgt
    19081 gctgacgctt tgggggggcg cgaccagccg cacgctgcgg gcgcactgga tgctgcacga
    19141 gctggggctc gactacgagc cccgcctgat cggctcgcgc acgggcgcca cccagacaga
    19201 cgccttcaag cagctcaacc ccaaggagaa gatacccgtg ctggtggacg acgaattcac
    19261 gctcaccgag agcgtggcca tcgtcacgta cctgggcgac acctacggct ccggcagcgg
    19321 tctcgtacct gagcctttca cccggaaccg ggcgcgctac aacgaatggg ccagcttcat
    19381 ccagatggag ctcgacgcgc acacgctgta cgtcctgcgc aaacaccggg acctggcagg
    19441 catctacggc gaagcccccg ccgcggtggc taccgccatt ggcgggtttg ccaagcaggt
    19501 ggccgtggca gacgaagcgc tcagggggtc ggctcatctg atcggcagca agttcagcgc
    19561 agcagacctg ctgctggtca gctgcctgga ctgggccgcc gcctacgggc tcgagctcac
    19621 gcccggcctc gacgagtacc ggttgcggat ccgcaagcgg cccgcttacc gggctgcggc
    19681 ccggctcaac ttctctatat ctgccggagc ctgagccctc agggagccga aggccccatc
    19741 agccagtcca ggatgcgggc gttctcctcc cggggatagg tgtgggatag atcgtcgacc
    19801 tcgcggtagg tgagcgcggc tccagctgcg gtcaaggcat cgcgcgccgt gcgcgccacg
    19861 tcgacgggga acatccagtc cagcgtcccg tgcacgaggt atatgggcag gccggtcagg
    19921 cgatcccgat ccacgccttc cagaatgaag ggatgaaagc tggccgagat gggcgagaga
    19981 tgggtgaacg gggtatccga gcgcaggccg ctcagatagc tgaaggttcc gccgtcggac
    20041 atgcccgtga gcagtatgcg gcgctcgtcc acgcgccaat gctcgcagac ccgttcgacg
    20101 atggcgtcca ggctcgcgct gtccgcatcg gagcctgcca gcgaccacgt tcggtcgcgg
    20161 gaggtgggcg cgaccaggag cacgccccgt gagcgggcat ccttcaacca gttccagaga
    20221 aagctgcgcc cgtgaccgct gccgccgtgc agggcgacca ccacaggcca cgcctcgccg
    20281 gcgtcgtagt actccggaac gtagagggaa aaaccgcccc gctcaccagg tccgttgtgc
    20341 gcatgcatca cccccacgtc gtcccggcca ggatccgccc gctccaggcg tctcgccaac
    20401 gcgccgtcgt cgcggccggc ctcggtcaga aaaaagcggc tgaccggggg gagcatgaag
    20461 gagaccgggt acaggcgttc ggtagcgcgg gtcgcggcac ccatggcctg gtaggcggcg
    20521 agcaccgggt tcgcctgctc taggcacgcg ctcatcgcgt ccatggccgc gagcgcttca
    20581 cccgcagaag cgctgatgtg atcgcgaaac cgctccaggt ggggcggcca cgggatctct
    20641 tcgaacttgc gcgcgccctg ccggagcggc cgggcgaacg cgtcgatctg aggaaccaga
    20701 tgcgccaggg actgaggatg caggtgcctg cccgcatggg cgagggcatc cagcgcggtg
    20761 agcagcggtg gaaccagcgc catggttgct tcgagcagga cgtcggtgtc ttggcccatc
    20821 agcgcactat accctgccga tggaccggct gagcgccggc gcccgccgcg ttggatcggc
    20881 gtcggcagat tgattaaagt catgggttgc gccactcgaa gatcgtcaat tgcccgagta
    20941 cgaccgcagc gccgaggacg tcggcaacgt gcagatgctg gagcacgtca acgtgaccgt
    21001 tcccgatcag gggctggccg cgctgttcta cgtgaccggt ctcggcttca cccgcgaccc
    21061 gtacatcgac ttcggcacgt acaacatgtg ggtcaatctc ggtgagcagc agtttcatct
    21121 cccccagtcg gacgcccaga tccttcgcgg cgagatcgca gtggtcgttc caaacctcga
    21181 agatctcgcg aaaagactga agttcgtctc caaggcgttg aaggacaccc gcttctcgtg
    21241 ggaagaggca gaccaaaggg tggtgctcag ctgtccctgg ggcaatcgca tcgtcgccta
    21301 ccccgccgga tcttttcccg ccatggacct gggaatgccc tacgtggaca tgtccgtccc
    21361 gcccggaacc gcccctggca tcgcacggtt ctacgaatcg gtgatgggct gcccggtatc
    21421 cgtggagccc ggccgcgtca aggtggccat gggctacaac cagaagctga tctacacgga
    21481 gaccgaagcg cctctcgccc cctacgacgg tcaccacatc gccatctacg tcagcgattt
    21541 ttcaggtccc caccgctttc tgcgcgaacg cggcctgatc agccaggaat cggatcagca
    21601 ccagtaccgc tttcagaaaa tcgtcgatcc cgccgacgat cgcggcgagg ccgagccgct
    21661 gttcgagctg gagcacgagg tccgcagcct tggccatccc atgtaccggc gaccgctggt
    21721 gaaccggaat cccgcggtga acttcttcac ctatcgcagg ggcaacgagg ccttcactcc
    21781 cggatgagcg aagcagccaa actcgcccgg ctcgcagaag agccctacta ccttccgctc
    21841 gccgacgagg tggccgtctt ccgctccgcc tacgaggctc gtctgcccgt gctgctcaaa
    21901 ggcccgaccg gctgcggcaa gacgcgcttc gtggagcaca tggcctggcg cctgtaccgg
    21961 gatacccaga accccggtgc cgagctcgaa gtgccgctgg taaccatctc ctgtcacgag
    22021 gacctgaccg ctacggacat ggtgggtcgc ttcctcctgc agggagactc gacgatctgg
    22081 agcgacgggc ccctgacccg ggccgttcgg accggcgcca tctgctactt agacgagatc
    22141 gtcgaggcgc gcaaggacac cacggtgctg atccactcgc tgaccgatca ccgccgcctg
    22201 ctccccatcg agaaaaccgg cgagctggtc cccgcgcacc cgggcttcct gctcgtgatc
    22261 tcctacaacc cgggttacca gagcgtgctg aaagacctga agacaagcac acggcagcgg
    22321 ttcgtgagcc ttacgttcga ttacccggac cgggagcgcg aagccgagat catcgcccac
    22381 gaatccgacg tcgagcccgc cgtggcggaa cgtctggcgg ccctgggcga aaaggtccgc
    22441 aacctgcgcg agcacggctt cgaggaaggg gtgagcaccc gccttctcat ctacgccgcc
    22501 cagctcatcg agcggggaat cccttcccgc cgggcggtgg atgccgcggt ggtccaggcg
    22561 gtatccgacg atgccacggt gcagcaggcc atagccgata tcgtcgacgc cgttctgcca
    22621 tgacggccct ggtcgatgtg gaagagccgc tggcgctctt cgccgagggc ataaccgggc
    22681 gctactacca catcaaggcc gttggcgagt attcgggcaa ccggatggtc ttcgatccgg
    22741 cgcgcggcgc gttgacgggc gacgcgctgc tgctgcccga aaccttcgaa atggacgacc
    22801 cggcctgcta ccgggtgctg gctttggagc agctgagcat cgtggagttc ggcacgctga
    22861 aattctccat ggccgaagca agacttcgca tccccgcttt agagcccccg ggggagccgc
    22921 ccatcacgga cagagcgagc gacctcgagc gcttcttcaa tcacgtctcc cgaccccacc
    22981 tgctgagccg gcttttcgcc ctctgcgagc gggtccggct caacgcccaa gtaagagacc
    23041 agtaccccgg gatcaggcgg catcaggacg cgctgcacgc cgtgctgagg cagagcgcgc
    23101 cgcggggcga cacgtcgtcc gtcgccgcca ttctcgacgg tctgggttac cgggtcctgg
    23161 gtgggcggaa cgcggccttg gaatcccatc cgctggcagg cgccctgctc gaattgatgc
    23221 tcggcgtggc tcgtctggac gccgacgtct tcgactcggc acgcgctgcc cacgcctgct
    23281 accggctgtg catggcatcc gccgccaccg cggaggccgt ggcggtgctc gaggaagacc
    23341 tggacgacgc ggccagctgg cttcagcggg acgtccggct cgaggactgg gaggagggcc
    23401 tcaagcagat gaacggcctg atcctggcgg ccgagacggc agaaggcgag gaggccgtcg
    23461 ctgcggacgt ggagggcctc tccgacggcg acatccgccc tgcggacgtt gacctgcggg
    23521 cgctgagcaa cgagcgcgac gtccttgccc gacggctcga catggagcgg gcaacgattc
    23581 gcgacgccgt gggcaggcct cgaccggatg ctcgcagcta ccgctacgac gagtgggact
    23641 atctgaaccg ctcgtaccgc acgcgatggt gccggctgtt cgaggagcgc ctcgccccgg
    23701 acacacagca ggacctcgac gcgctgcggc atgtgatccg ccgccaccgc cgggcagtgc
    23761 agagccagct ggagcacatc aagccccagg gatatcagcg ggtcacccgg gtggcggacg
    23821 gtgacgagct ggacttcaac gcggtgatcg aggcccgcgg cgacatccgc gcagggctta
    23881 ccccggacga gcgcgtctac agccgccggg aacgcgtgca ccgggacgtc tgcgctgcct
    23941 ttctcgtcga tctgtccgcg tccacggacg atccggtgga tccgcccgaa gccgtcgcgg
    24001 ccggcgcggt cgaggagctc gccaacctgc gcgatcccta cgaatttccg gtcgcccacg
    24061 acgagccggc cgaggagccc cgtcgcatca tcgacgtcca gagggaatcc atgctcgtca
    24121 tgtcggccgc cctggagcag ctcgcagaca gctacggcat ctacggcttt tccggatatg
    24181 gccgcgagtg cgtcgagttc ttcgtcgcca aggagccgga cgagccgttc acgcgtggca
    24241 ccctgtccgc cattgccggg atgaagccca agcgcagcac ccgcatgggc ccggccatac
    24301 gccacgcaac gtccaagctg ctgcgctcag gtcacgccct gaaggtgctg atcatcctga
    24361 gcgacggctt tccccaggac tccgactacg ggccggagcg gggcgatcat gaatacggcg
    24421 tgcaggacac cgccaaagcg ctccgggagg ccgaggaaaa gggcgtggag acgttctgcg
    24481 tcacggtgga ccgctcgggg cacgactacc tcaagcgcat gtgccctaac gcgcgctaca
    24541 tggtcatcga ggaaacccgg gatctgccgg gagcgctcag caaggtctac gaagcgctca
    24601 ccggctgatc aggatctgtc gcggcgttca gtcgtcgctg ccgaacgctc cgaaaatgtg
    24661 cagcaacgag atgaacaggt tgtagatctg cacgtagagg gtgatggtgg cgagtatgta
    24721 gttggtctcg ccgccgtgga tgatggcgct cgtctgccag agaatggccg cgcaggcaaa
    24781 cagcacgaag ccgcaggata tggccaggtg gaagccggag aggtcaaaaa gccaggccag
    24841 cagcaccgcg cccatcagaa cgaagaagcc gaccacgagg aagccgctca aaaagctgaa
    24901 gtccttgcgg gtgaccagcg cgaacgcgga gagcccgacg aaggcgagcg ccgtggtgcc
    24961 cagagccgag gcgatcagga cgtcaccgtt ggcgagcgtc agatacgcgt tgagaatcgg
    25021 accgagcccg tagcccagaa agcccgtgaa ggcgaaggtc cagaagaggc cccaggggct
    25081 attggccgtc ttgtgcacga tgaacagaag accgaagaag ccgaccaggg ttgggagaaa
    25141 accgaggtag ggggcatctg cgatcatcgc cgcggtggcg accaccgcgc tgaacgccag
    25201 cgtgaggccc agcagcatgt aggtattgcg cagaaccttg ttgatctcga tggccggagc
    25261 ggcggccgcc ctgaccgaag acacttcgta atttgccatt ttctttactg aatccttact
    25321 gctaatcgtt cccagg
//
LOCUS       DBNHLM010000052         2939 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_665061, whole genome shotgun sequence.
ACCESSION   DBNHLM010000052 DBNHLM010000000
VERSION     DBNHLM010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_665061"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="AC16QF_02760"
     CDS             <1..230
                     /locus_tag="AC16QF_02760"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR6447503.1"
                     /translation="NGKTIFLDHGQGLVTMYCHLSEISVEPGEAVERGATIGLVGATG
                     RATGPHLHWSVSLNGNRVDPELVISALAVGD"
     gene            complement(268..1674)
                     /gene="der"
                     /locus_tag="AC16QF_02765"
     CDS             complement(268..1674)
                     /gene="der"
                     /locus_tag="AC16QF_02765"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR6447504.1"
                     /translation="MVPTVALVGRPNVGKSTLFNKLTRRRDALVADVPGLTRDRRYGR
                     MPAGAGECMLIDTGGLFGEPGALQSALEQQAELALDEADAVLFVTDARDGLVPADEEI
                     AQRLRRLSKPVILVVNKMDGVQADVAMADFAALGFDAVAYVSAVHERGLGTVRELVEE
                     RCPHGPTGEEAADESDAVRVAVIGRPNVGKSTLVNRMLGQERQVVFDEPGTTRDAIEV
                     PFEKDGKRYVLVDTAGVRRKGRVEGVAEKFSVVKTLQAIDLAHVVILVLDAREGVVDQ
                     DLHLLSHAAEAGSGLVVAMNKWDGLSVEERTNARRVLDRKLSFAPWIPVMQISALHGT
                     GVGHLLEEVDRVYKAGELDVSTSVLTRLLLGMTGAHPPPAVRGRSIKLRYAHKAGSHP
                     PRIVIHGNQTEAVPDSYRRYLENGFRKALALVGNPVHIELRTGDNPYAGQRNELTPRQ
                     KARRKRMIQHRKRKAKKR"
     gene            complement(1682..>2939)
                     /gene="bamB"
                     /locus_tag="AC16QF_02770"
                     /gene_synonym="yfgL"
     CDS             complement(1682..>2939)
                     /gene="bamB"
                     /locus_tag="AC16QF_02770"
                     /gene_synonym="yfgL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HIR6447505.1"
                     /translation="NPPGRLQKRPMRKILLLLLTFSLTGCSWFSWLPWVGDKDDEKKK
                     LEPAKLVDFDAEVNVDRVWRASIGEGLGKKYLRLDPLVLADRVYAADGYGIVEALDRF
                     SGKRVWRSRIGRVEEGLLGSINFFDREDPSFVSGGVGGGAGYVFMGTTGGDVVALSAA
                     DGSEAWRSHVGSEVLAPPVGERDLVFVQTQDGRLLALESETGDIRWSFDNQVPVLTLR
                     GTGTPVVAGNVVFAGFSNGMLAALRAESGEPVWEQRIMLPEGRSELERMVDVDGTPLL
                     LGPILYVVAYQGKLKALRRTDGAMLWEMDVSSFLDLAEGYGQIYVVDDGDVVTAVDQQ
                     SAEIVWQQPGLARRKLTSPVAFSNYVAVGDDQGYLHILAQSDGRFLGRRKLDGDGLRS
                     SMAVVDGVLYVLGNSGSLHALDIEVK"
BASE COUNT          520 a         1024 c          892 g          503 t
ORIGIN      
        1 tcaacggcaa gacgatcttc ctcgatcacg gacagggact ggtgaccatg tactgccacc
       61 tgagcgaaat cagcgtggag cccggtgagg cggtggaaag gggcgccacc ataggtctgg
      121 tgggcgccac cgggcgggcc acgggacccc acctgcactg gtcggtcagc ctaaacggta
      181 accgggtcga tcccgagctg gtaatctctg ccctggccgt cggggactag cccaccttcg
      241 gcaccaccgg gcgggagagg cctccggtca gcgttttttt gctttgcgct tgcgatgctg
      301 aatcatccgt ttgcgacggg ctttctgacg gggcgtgagc tcgttacgct ggccggcata
      361 aggattgtct ccggtgcgca gctcgatgtg tacggggttg cccaccaggg cgagagcctt
      421 ccggaagccg ttttccaggt atcttcgata gctgtccgga accgcttcgg tctggttgcc
      481 gtggatcacg atccgggggg gatgcgatcc ggccttgtgg gcgtagcgca gcttgatgga
      541 acggccacgg actgcgggtg gcgggtgggc gcccgtcatc ccgaggagca gccgggtcag
      601 cacagacgtg ctgacgtcga gctctcccgc tttgtagacc cggtcgacct cttcgagaag
      661 gtggcccacg ccggtgccgt gcagggccga tatctgcatc accggaatcc agggtgcaaa
      721 agacagcttc cggtccagaa cccgtctggc attcgtcctt tcctcgaccg agagcccgtc
      781 ccacttgttc atggccacca ccagccccga gcccgcctcg gcggcatggc tgagcaggtg
      841 cagatcctga tccaccaccc cttcccgggc gtcgaggacg agtatgacca cgtgggccag
      901 atcgatggcc tgcagggtct tcaccaccga gaacttctcg gccaccccct cgacccgccc
      961 tttgcgccgc acgccggcgg tatccaccag aacgtagcgc ttgccgtcct tctcgaaagg
     1021 cacctcgatg gcgtcacgcg tggtgccggg ttcatcgaac accacctggc gctcctggcc
     1081 gagcatgcgg ttcaccagcg tggatttccc cacgttgggg cggccaatca ccgcgacgcg
     1141 cacggcatcc gattcgtcgg ctgcttcctc cccagtcggg ccgtgggggc accgctcctc
     1201 caccagctcg cgtacggtgc ccaatcccct ctcgtgcacc gcagagacat aggccacggc
     1261 atcgaagccg agggcggcaa agtcggccat cgcaacgtcc gcctgcacgc catccatctt
     1321 gttgaccacc agaatcaccg gtttggaaag acgcctcagg cgttgggcga tctcctcgtc
     1381 agcgggcacc aggccgtccc gggcatcggt cacgaacagc accgcgtcgg cttcgtcgag
     1441 tgccaactcg gcctgctgct cgagggccga ctgcagcgcg cccggttccc cgaagagccc
     1501 cccggtgtcg atgagcatgc actcaccggc ccctgccggc atgcgcccgt agcgccgatc
     1561 ccgggtcagg ccgggaacgt cggccaccag agcgtcccgg cgcctggtca gcttgttgaa
     1621 gagtgtggac ttaccgacgt tgggccggcc aacgagggcg acggtgggaa ccatcccggg
     1681 cctatttgac ctcaatgtcg agcgcatgga gggaaccgga gtttccgagc acgtaaagca
     1741 cgccgtccac gaccgccatc gacgaccgca gcccgtcccc gtcgagcttg cggcgcccga
     1801 ggaagcgccc gtcactctgc gcaaggatgt gcagatatcc ctgatcgtcg cccaccgcga
     1861 catagttgct gaaagcaacg gggctcgtga gcttacgacg cgcaaggccc ggctgctgcc
     1921 agacgatctc cgccgactgc tggtcgacgg ccgtcaccac gtccccgtcg tccaccacgt
     1981 agatctggcc gtatccttcg gcgagatcca ggaagctgga cacgtccatc tcccacagca
     2041 tggctccgtc ggttcgtcgc agcgccttga gcttaccctg gtaagccacg acgtagagga
     2101 tcggacccag gagcaggggc gtgccgtcca cgtccaccat gcgttccagc tcggaccttc
     2161 cttccggaag catgatccgc tgctcccaga cgggctcgcc gctttcggcg cgcagagcgg
     2221 cgagcatccc gttggaaaag cccgcaaaca ccacgttacc ggccaccacc ggggtgccgg
     2281 tgcctctcaa ggtcagcacc ggaacctggt tgtcgaagct ccagcgaatg tccccggtct
     2341 cggattccag cgccagcagc ctgccgtcct gagtctgcac gaacaccaga tcgcgctcgc
     2401 ccaccggtgg cgcgagcacc tcgctgccca cgtgggaccg ccaggcttcc gaaccgtccg
     2461 cagcggacag cgccaccaca tcaccgcccg tcgtgcccat gaaaacgtag cctgcaccac
     2521 cgccgacccc gccactcacg aaactcggat cttcacgatc gaagaagttg attgagccca
     2581 ggagaccttc ttcgacgcga cctatgcgcg accgccagac ccgcttgccg gaaaaacggt
     2641 cgagcgcttc cacgatgcca tagccgtccg cggcgtacac ccggtcggcg aggaccaggg
     2701 gatcaagtcg cagatacttc ttgccgagac cctcaccgat ggaggcccgc cagacccggt
     2761 cgacgttcac ctcggcatcg aagtccacca gcttggccgg ctcgagcttt ttcttctcgt
     2821 cgtccttgtc gcccacccac ggcaaccagg aaaaccagct gcagccggta agggagaaag
     2881 tcagcaggag cagtagaatt ttacgcatcg ggcgtttctg cagtctcccc gggggattc
//
LOCUS       DBNHLM010000053         5167 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_784479, whole genome shotgun sequence.
ACCESSION   DBNHLM010000053 DBNHLM010000000
VERSION     DBNHLM010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_784479"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1081)
                     /locus_tag="AC16QF_02775"
     CDS             complement(50..1081)
                     /locus_tag="AC16QF_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6447506.1"
                     /translation="MRSQKSSQKVTIVDVAKRAGVSIKTVSRVLNSEPNVRADTREKV
                     LKAAKALHYQPNPSARGLAGNRSFSIGLLYENPQEFGYVKDALNGVIGTCEAEGYSLL
                     MRPCGEEVDSEEVRKFVAQTRVDGAVLTAPVCDEPKVTDLLKELGVPFAQLAPKAPRS
                     EWISVQCNDEEASYAMTDYLISLGHRRVGFIKGHPDHGATEKRLAGYRRCLADHDMPF
                     DKTLVMQGYFDFESGQRCARKLLELPNPPTAIFASNDDMAAGVIFTARERGIGVPDEL
                     SVAGFDDSPAASHTWPPLTTVKQPIVEMAARATQRLIGQLRGVGEEGDEEIFTCELIV
                     RRSTAKRGD"
     gene            complement(1146..2633)
                     /locus_tag="AC16QF_02780"
     CDS             complement(1146..2633)
                     /locus_tag="AC16QF_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6447507.1"
                     /translation="MSSAEHYATAPEDRISFAHKVVYGFGAFVNNLLAAAIGGMAIVL
                     NLGLGMNPALVGLLGALPRLTDALTDPLMGYISDHTNTRWGRRRPYIFAGAIAAGLCF
                     ALLWQLPPGRSESFYFYYFLFGSLIFYLAYTVFATPWVALGYELTPDYHERTRLMGVQ
                     NFIGQLAYLISPWFLWMMQYEPVFDDMVEGAAGLSVAIAVVVMCVGVLPALLLRERFR
                     DVAHAEIAEKQLARGEPRHPLLTNVKSFFAGFAATLRFKPFVRLCAATFLVFNGFMLV
                     SSFQFYVIIYYVFSGDQVTGAEYAGWSGTLSAVSTFVVIGIVTWLATKIGKRRAFFAA
                     TGISIVGYGLKWFCYDPAVPWLLLVPAPLIAFGLGGLFTLMGSMIADVCDLDELASHE
                     RREGMFGSIFWWVVKLGMALALAGGGFLLNATGFDVALGGAQPASAILMMRAFDVIVP
                     MLSSAAAIWIIAGFPITEERAHQVRARLEERRGALNGDPGTAAAV"
     gene            complement(2676..3305)
                     /gene="eda"
                     /locus_tag="AC16QF_02785"
     CDS             complement(2676..3305)
                     /gene="eda"
                     /locus_tag="AC16QF_02785"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - (R,S)-4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIR6447508.1"
                     /translation="MDACDLLDGVGIVAVVALEDADAAVPLARVLAGSGVSCVEVTLR
                     TGCAVDAIGRIAREVPEVLVGAGSIRRPEQLLEVRDAGARFAVSPGSSDGLLAASRRL
                     KMPFVPGAVTATEMIGLLEHGYFLQKFFPAEASGGARALRSLAAPLPEVRFFPTGGIT
                     QELAPGYLQMGSVACIGGSWFVPADSLRAGDFSAIGRLAGAAAELTTLS"
     gene            complement(3307..5112)
                     /gene="edd"
                     /locus_tag="AC16QF_02790"
     CDS             complement(3307..5112)
                     /gene="edd"
                     /locus_tag="AC16QF_02790"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HIR6447509.1"
                     /translation="MNPLVERVTERIRERSASSRSDYLRRIEEAAASGVTRARLSCSN
                     LAHGMAACPAGSKTRLSGEAAANVGIVSAYNDMLSAHQPYERYPELIRRAAEDAGAVA
                     QVAGGVPAMCDGITQGRPGMELSLFSRDVIAMSTALALSHDMFDAVVCLGVCDKIVPG
                     LLMGALAFGHLPAVFVPAGPMPSGLPNKEKARVREAYAAGEAGRVELIAAESAAYHSP
                     GTCTFYGTANSNQMLLEFMGLQLPGGSFVNPGSELRDLLTAAAVRQVLELTRLGNRHM
                     PIGRIVDESSVVNAVVGLLASGGSTNHTMHLVAVAAAAGIRLDWSDFADLSRVVPLMA
                     RVYPNGEADVNHFHDAGGLGFMIGELLDHGLLHDEVETILGRGLSRFVVCPERADGAL
                     RWRPAKKSSADPGVLRGVAEPFDAEGGLRVVQGDVGRAVVKVSAVRAERRRISAPARV
                     FVSQDAFAEAFEAEELEGDMVAVLPWQGPRANGMPELHRLSPYLGVLERRGYRVALLT
                     DGRMSGASGAVLAAIQVTPEAASGGLIGRIRDGDLIHIDADAGVVRVEADLEGRTGAA
                     AEAPDGTHGTGRELFAAFRAQVGPAESGASIFGPL"
BASE COUNT          956 a         1770 c         1673 g          768 t
ORIGIN      
        1 ggcccgcggc ggatctcggc caggcgtctc ggcccgccgt cagcccgctt cagtcgccgc
       61 gcttggcggt ggagcgtcgc acgatgagct cgcaggtgaa gatttcctcg tcgccctctt
      121 cacccacacc ccgcagctgg ccaatgagcc gctgggtcgc tcgtgcagcc atctccacga
      181 tgggctgctt gacggtcgtc agcggcggcc aggtgtgcga cgccgccgga gagtcgtcga
      241 atccggccac ggagagctcg tcgggaacgc cgatgccgcg ctcacgcgcg gtgaaaatga
      301 cgcccgccgc catgtcgtcg ttgctggcaa agatggccgt tggcgggttc ggcagctcta
      361 ggagcttccg ggcgcagcgc tgaccggact cgaaatcgaa gtagccctgc attaccaggg
      421 ttttgtcgaa gggcatgtcg tgatccgcga ggcagcgcct gtaacccgcc agccgcttct
      481 ccgtcgcgcc gtgatcggga tgccccttga tgaaaccgac gcggcggtga ccgagagaaa
      541 tcaggtaatc ggtcatggcg tagctggctt cctcgtcgtt gcactgcacc gagatccact
      601 cggatcgggg cgctttcggg gcgagctgag caaacggcac gccgagctcc ttcagcagat
      661 cggtgacctt gggctcgtcg cacacgggtg ccgtcaacac ggcgccgtca acgcgcgtct
      721 gcgccacgaa cttgcgaacc tcctccgaat ccacctcctc gccgcaaggc cgcatgagga
      781 gcgagtaacc ctccgcctcg caggtaccga tgacgccgtt cagagcgtcc ttgacgtaac
      841 cgaattcctg gggattttcg taaagcaaac cgatcgagaa cgaacggttg ccggcaagcc
      901 cgcgcgccga ggggttcggc tggtaatgca gggccttggc ggccttgagc accttttccc
      961 gggtgtccgc acgcacgttg ggctcgctgt tgagcacccg ggacacggtc ttgatggaca
     1021 cgcccgctcg cttggccaca tcgacgatcg tgaccttctg gctgctcttc tgcgatctca
     1081 tgcgtactcc gtttccaggc tcagcggtaa aaacccatca gcacactttc gaaacgccgc
     1141 tcattctata cggccgcagc cgtccctgga tcgccgttca gggcaccccg gcgttcctcg
     1201 aggcgcgctc gtacctgatg ggcacgttct tccgtaatcg ggaagccggc gataatccag
     1261 atagccgccg cggagctcag catgggtacg atcacgtcga acgcgcgcat catcaggatc
     1321 gcgctggccg gctgagcgcc gccgagcgcc acgtcgaagc cggtggcgtt caagagaaac
     1381 ccgccgccgg cgagcgccag cgccatgccg agcttcacga cccaccagaa aatcgacccg
     1441 aacatgcctt cgcgccgttc atggcttgcg agctcgtcca ggtcgcagac gtcggcgatc
     1501 atggagccca tcagggtgaa cagaccgccc aggccgaacg cgatcagcgg cgccggcacg
     1561 agcagcagcc acggcaccgc cgggtcgtag cagaaccact tcagcccgta ccccacaatg
     1621 gagatccccg tggctgcaaa gaaagcccgc cgcttgccga tcttggtggc aagccaggtg
     1681 acgatgccga tgacgacgaa cgtggagacg gccgacagcg tgccggacca gcccgcgtac
     1741 tccgcgccgg tcacctgatc accggaaaag acgtagtaga tgatcacgta gaactggaag
     1801 gaggagacca gcatgaagcc gttgaagacg agaaacgtgg cggcgcacag cctgacaaac
     1861 ggcttgaacc tgagcgtggc ggcgaagccc gcaaagaagg acttcacgtt ggtcagcaag
     1921 ggatgccggg gttccccacg ggccagctgc ttttccgcga tctcggcatg agcgacgtcc
     1981 cgaaaccgct cgcgaagcag caacgccggc agaacgccca cgcacatcac caccacggcg
     2041 atggctaccg acaaccccgc ggcgccctcc accatgtcat cgaagaccgg ctcgtactgc
     2101 atcatccaca ggaaccaggg agagatcaga taggccagct ggccgatgaa gttctgaacc
     2161 cccatgagcc gggtgcgctc gtgatagtcg ggcgtcagct cgtatcccag cgcgacccag
     2221 ggcgtggcga aaaccgtgta ggccaggtag aagatcagcg aaccgaagag gaagtagtag
     2281 aagtagaagc tttcgctgcg acctggcggc aactgccaca gaagggcgaa gcacaacccg
     2341 gcggcgatgg cgcctgcgaa gatgtaaggc cgccggcgcc cccaccgcgt gttggtgtgg
     2401 tccgagatgt atcccatcag ggggtctgtg agcgcatcgg tgagtcgcgg caacgctccg
     2461 agcagcccca ccagagccgg gttcatgccg agacccaggt tcagcacgat ggccatgccg
     2521 ccaatggcgg ctgccagcag gttgttgacg aaggccccga acccgtagac gaccttgtgc
     2581 gcaaagctga tccggtcttc cggtgcggtg gcgtagtgct cggcggaact catcggtagg
     2641 tgcgacctgt gggcttccga ccgattaggc ccgctctatg acagcgttgt caattcagcc
     2701 gcggccccgg ccaggcggcc aatcgcagag aagtcgccgg cccgcaggct atcggcagga
     2761 acgaaccagg atccaccgat gcaggccacc gagcccatct gcagataccc gggggccagt
     2821 tcctgggtta tcccgccggt gggaaagaag cgcacctccg gcagcggcgc ggccagcgag
     2881 cgaagcgctc tggcgccgcc gctcgcctct gcggggaaga atttctgcag aaagtaaccg
     2941 tgctccaaaa gcccgatcat ctccgtcgcc gtgacggcgc cgggcacgaa gggcattttc
     3001 aaccggcgcg aagccgccag aagcccgtcg ctcgagcccg ggctcacggc aaagcgggcg
     3061 ccggcatcgc gcacctcgag gagctgctcc gggcggcgga tgctgcccgc cccgaccagc
     3121 acctcgggaa cttcccgggc gatccgcccg atggcgtcca cagcgcagcc cgtgcgcagg
     3181 gtgacctcaa cgcagctgac cccggaaccg gccagaactc gcgccagcgg cacggcggca
     3241 tcggcgtcct ccagcgccac caccgcgacg atcccgacgc catcgagcag atcgcaagcg
     3301 tccatctcaa aggggcccga agatgctggc gccggactcg gccgggccga cctgggcgcg
     3361 aaaggcggcg aatagctcac gaccggtgcc gtgggttccg tcgggcgctt ctgcagccgc
     3421 ccccgttcgg ccctccagat ccgcctcgac gcgcacgacg cccgcatcgg cgtcgatgtg
     3481 gatcagatcg ccgtcgcgaa tccgaccgat cagccccccg ctggcagcct ccggcgtcac
     3541 ctgaatggct gcgagcacgg cgccggaggc gccagacatg cggccgtcgg tcagcagagc
     3601 gacccggtat ccccgacgct ccagcacgcc taagtagggg gagagccgat gcagctccgg
     3661 catgccgttg gcgcgcggac cctgccaggg cagcacggcg accatgtcgc cctcgagctc
     3721 ttcggcctcg aatgcctcgg caaaggcgtc ctgggagacg aacacccggg cgggggccga
     3781 gatgcgccga cgctccgccc ggaccgcgga taccttgacc accgcgcgcc ccacatcgcc
     3841 ctgaaccacc cggagccccc cttcggcgtc gaacggctcc gcgaccccgc gcagcacgcc
     3901 gggatcggcg gacgattttt tcgcgggccg ccacctcaag gcgccgtccg cccgctcggg
     3961 gcatacgacg aaccggctga ggccgcggcc caggatggtt tccacctcgt cgtgcagcag
     4021 gccgtgatcg agcagctcgc cgatcatgaa gccgagcccg ccggcgtcgt ggaagtgatt
     4081 gacgtcggcc tccccgttcg gatagacgcg cgccatcagc ggcacgacgc gggacagatc
     4141 ggcgaagtcg gaccagtcca gccggatgcc ggcggcggcg gcaacggcca caaggtgcat
     4201 ggtgtgattg gtggagcctc cggaggccag caggcccacc accgcgttga ccaccgaaga
     4261 ctcgtccacg atgcggccga tgggcatatg gcggttgccc agacgggtga gctccagcac
     4321 ctgtcggacc gcagcagcag tgagcagatc gcgcagttcc gagcccgggt tgacgaaaga
     4381 tccccctgga agctgcaggc ccatgaactc caagagcatc tgattgctgt tggccgtacc
     4441 gtagaaggtg caggtgccgg ggctgtgata ggcggcagac tcggccgcga tgagctcgac
     4501 cctgcccgcc tccccggcag cataggcttc tcgaacgcga gccttctctt tgttcggcag
     4561 gcccgacggc atggggccgg cggggacgaa caccgcgggc aggtggccga aggccaacgc
     4621 gcccatgagc agaccgggaa cgatcttgtc gcagaccccg agacagacca ccgcatcgaa
     4681 catgtcgtga gagagcgcca gcgccgtgga catggcgatg acgtcgcggc tgaagagcga
     4741 cagctccatg cccggacggc cctgggtgat gccgtcgcac atggcgggca ccccgccggc
     4801 cacctgggca acggctcccg catcctcggc ggcccttcga atcagctccg gatagcgctc
     4861 gtacggctgg tgagcgctga gcatgtcgtt gtaggccgac acgatgccga cgttggcggc
     4921 ggcttccccg gagaggcgcg tcttggaccc ggcagggcag gcggccatcc cgtgggccag
     4981 gttgctgcag gacagccgcg cccgggtcac tccggaggcg gcggcctctt cgatccggcg
     5041 cagatagtcg ctacggctcg acgcgctgcg ctcgcgtatc cgctccgtca cacgttcaac
     5101 caaaggattc attcactttt cctcgagcca tcgatttcca gcccacttcc ccacttcacg
     5161 gggccca
//
LOCUS       DBNHLM010000054         4562 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1024314, whole genome shotgun sequence.
ACCESSION   DBNHLM010000054 DBNHLM010000000
VERSION     DBNHLM010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1024314"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..890)
                     /locus_tag="AC16QF_02795"
     CDS             complement(66..890)
                     /locus_tag="AC16QF_02795"
                     /inference="COORDINATES: protein motif:HMM:NF025007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HIR6447510.1"
                     /translation="MAEFRSILHGDRPAVIDGLLNLSPDLARWTPEFLAERYGERSVR
                     VYDASFGEPGSNYMGSISSMSFAEFLNAIFGAGRDLRMFLHNVRAGFPEMLEDVVFPE
                     AGLRFSRNFVYSFFGCRGATTPLHYDIDMGCVLHTVLHGRRRIRLFALDQSTRLYRHP
                     FTVRSYVDLDRPDFQAHRALSAAQGAEVILEPGQTLFMPAGCWHEFHYLEPGFGLSLR
                     ASSPRLKDRAAGALNLLLLSPTDRLMNKLSPQAWFKWKQSIASRRAAAHLETSGQA"
     gene            1187..1969
                     /locus_tag="AC16QF_02800"
     CDS             1187..1969
                     /locus_tag="AC16QF_02800"
                     /inference="COORDINATES: protein motif:HMM:NF016974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HIR6447511.1"
                     /translation="METTPGQRRSVRDALGYLFRRAVFWFGFYIRPPIHWFIGRFSKV
                     GNPPVFDPDVFAWTPELARNWEAIRDEALAVLENPADVPPLRTVSPDHSRIATDEKWK
                     VFFLWGYGYEIEGNTVRCPVTSRLVRAVPGLISAFFSIHTPGTHLPRHYGPTNGMITC
                     HLGLQVPEDSHGCRISIDDKDYNWEPGRFLVFDDTYYHEVWNDTDEDRVVLLMHVERP
                     LRQPGKAVADGFLWLATKSPFIQATLKALDEWTATQEKDPAA"
     gene            2164..2937
                     /locus_tag="AC16QF_02805"
     CDS             2164..2937
                     /locus_tag="AC16QF_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447512.1"
                     /translation="MKVMIFTLSMLMASVAFADGHGESWSGLNLSEGSAMQMQLCSLQ
                     PRKTMADYDKVFNAYIEWSKENDVQTYAMRAMPLFGGPDPAAGLDFQWIDMLVSPFAV
                     SGNGWDKWMTTESGQKLDKQWRETANCRVAIHTVASMFLDREALTHDSRIMSLNWCTR
                     HDGVTWDNLMARHATMAAGRPDGAPVKAWTLLFPGLGVRNAPGEFAHLLSFADTAGLM
                     AWENQLANGGGWRQREDYQSSYASCTGSNVYRTTILHRP"
     gene            complement(3017..3093)
                     /locus_tag="AC16QF_02810"
     tRNA            complement(3017..3093)
                     /locus_tag="AC16QF_02810"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3057..3059),aa:Arg,seq:cct)
     gene            complement(3168..4301)
                     /locus_tag="AC16QF_02815"
     CDS             complement(3168..4301)
                     /locus_tag="AC16QF_02815"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HIR6447513.1"
                     /translation="MDLLSQIWEGSGLNQLTWGQAIMIGVGLVLLYLAIARQFEPLLL
                     VTIGFGGILANIPGAGIAEGDGLLHLAYVVGIESGAFPLIIFMGVGALTDFGPLLANP
                     KTLFLGAAAQFGIFATLLGALGLSELGVFDFGLREAAAIGIIGGADGPTAIYVAGRLA
                     PELLGAIAVAAYSYMALVPLIQPPIMRALTTEAERKIEMVQLREVSRLERIVFPLLLL
                     LLVALLLPSAAPLLGMFCFGNLMRECGVVDRLSDTTQNALINIVTIFLGLAVGSKLSA
                     EQFLVPSTLGILALGMAAFALGTASGVLMGKLLNLVTREHINPLIGAAGVSAVPMAAR
                     VANRVGLEANRHNYLLMHAMGPNVAGVIGSAVAAGIMIMLVGD"
     gene            complement(4304..>4562)
                     /locus_tag="AC16QF_02820"
     CDS             complement(4304..>4562)
                     /locus_tag="AC16QF_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR6447514.1"
                     /translation="VKPAEPAFKAPVRAGAGTIIAALAGNVFKVLVQPGDVVTEGQTI
                     LVVEAMKMETAVAASQDGTVAEVHVREGDAVAVGDPLVTLA"
BASE COUNT          931 a         1471 c         1400 g          760 t
ORIGIN      
        1 gctgtcccca taccggcggt aaggcacctc gaagcgtcca atcttaagcg agcctttgac
       61 tgtcctcatg cttgacctga cgtttccaga tgggcggccg cccgcctgct tgcgatagat
      121 tgtttccact tgaaccatgc ttgcgggctg agcttgttca tcagccggtc cgtgggcgag
      181 agaagaagaa ggttaagcgc acccgccgcg cgatctttca gccgcgggct gcttgcccga
      241 agcgacaggc cgaaacccgg ctcgaggtag tgaaactcgt gccagcagcc ggccggcatg
      301 aacagtgtct ggcctggctc caggatgacc tccgctcctt gcgccgcgct cagtgcgcga
      361 tgcgcttgga aatcgggccg gtcgagatcg acgtagctgc gcacggtgaa gggatgacga
      421 tacaggcggg tagactggtc cagggcaaaa aggcgaatgc ggcgcctgcc gtgaagcacc
      481 gtgtgcagaa cgcaacccat gtcgatgtcg taatgcagcg gcgtggtggc tccccggcag
      541 ccgaagaagc tgtacacgaa gttacgtgag aaccgcaggc ctgcctccgg gaaaaccaca
      601 tcctcgagca tttcgggaaa gcccgcccga acattgtgaa gaaacatgcg aagatcgcgc
      661 cccgccccga aaatcgcgtt cagaaactcc gcaaaactca ttgacgagat actgcccatg
      721 taatttgaac ccggctcgcc aaaagaagcg tcataaaccc gcacggaacg ctcgccatag
      781 cgttcggcaa gaaattcggg tgtccagcga gccagatcgg gtgacaggtt caatagcccg
      841 tcgatgaccg ccggccggtc cccatggaga atggaccgga actctgccat ggcctctggc
      901 tcgaacgaaa ctctgaccac ctcgccaccc aacagatggg caggcgacga ggtcatcgag
      961 gatgtgtggt aggcggtccg gcaggctgaa tgtggagcgc ttcccgcgtt gataggaggc
     1021 aatttggctc gggccctgga gagcaggcgc ggagtttagc cgcttttgcc cccggacccg
     1081 actcggcgtc tgttacaaat tgtttcaggc cgcatgcaag gggcgacctt tggccctcac
     1141 gctggtgtaa ttaccggctc gaatccacga gacaggacca gaatccatgg agaccacccc
     1201 cgggcagcgc cgcagtgtcc gcgatgcgct gggctacctg ttccgccgtg cggttttctg
     1261 gttcgggttc tacatccgac cgcccattca ctggttcatc ggacgattct caaaggtagg
     1321 caacccacct gtcttcgatc ccgatgtctt cgcgtggacc cccgagctgg cgcgcaactg
     1381 ggaggccatc cgggacgaag cgctcgccgt gctggagaac ccggccgacg tgccgccgtt
     1441 gcgaaccgta tcacccgatc acagccgaat cgcaacggac gagaagtgga aggtcttctt
     1501 cctgtggggg tacgggtacg agatcgaagg caacacggtg cgctgccccg tgactagccg
     1561 cctggtccgg gcggttcccg ggctcatatc cgccttcttc tcgattcaca cgccgggcac
     1621 ccacctcccg cgccactatg gcccgaccaa cggcatgatc acgtgccatc tcggattgca
     1681 ggtcccggaa gattctcacg gttgccgtat ttccattgac gacaaggact acaactggga
     1741 accgggccgt tttctcgtct tcgacgacac ctactaccac gaggtctgga acgatactga
     1801 cgaggaccgg gtggttctgc tcatgcacgt ggagcggccc ctgcgacagc cgggcaaagc
     1861 ggtcgcagac gggtttctct ggctggccac caagagcccc ttcattcagg ccaccctaaa
     1921 ggccttagac gagtggacgg ctacccagga aaaagatcca gccgcctgac ctggctacta
     1981 tcgagccccg ccgtcgaccg gaagctctac catatgccgg cccgagcgac ccgctttaaa
     2041 tttcctgcgg aggctgcaat gatgcggacg tcatcgcggc aggccgacct tcgagcaacg
     2101 cgcggtacct ggggaggaac cggcggacgc gtagacccat cactcaatcg gggagaaaga
     2161 aaaatgaaag taatgatttt cacgctgtcg atgctcatgg cttcagtggc ctttgcagac
     2221 ggccatggcg aaagctggtc tggtttgaac ctgtctgagg gcagcgccat gcagatgcag
     2281 ctctgctcgc ttcagccgag aaagaccatg gcggactacg acaaagtctt caacgcctac
     2341 atcgaatggt cgaaggagaa cgacgttcaa acctacgcca tgcgcgcaat gcccctgttc
     2401 ggcggcccgg accctgcggc cggattggat ttccagtgga tcgacatgct ggtgtcgccc
     2461 ttcgcagtca gcggaaacgg ctgggacaag tggatgacca cggagtcggg tcaaaagctc
     2521 gacaagcagt ggcgggagac ggccaactgt cgggtggcca ttcacacggt ggccagcatg
     2581 tttctggacc gcgaggccct gacccacgac agccgcatca tgtcgctcaa ctggtgcacg
     2641 cgacatgacg gggtcacctg ggacaacctc atggcccggc acgccaccat ggcggccggc
     2701 cgtccagacg gcgcgccggt caaggcctgg accttgctct tccccggcct cggcgttcgc
     2761 aacgcgcccg gcgagttcgc ccacctgctg agctttgcgg ataccgcggg gctgatggcc
     2821 tgggaaaatc agttagccaa tggcggtggc tggcgccagc gcgaggacta ccagagctcg
     2881 tacgccagct gcacgggatc caacgtctac cggaccacaa ttctccacag accgtaaccc
     2941 caaccctaac gtcacgtcgc cacgctccgt ggcgacgtga cgatggccgc ggcccgttcg
     3001 ggaaagcacg aattactggc gccccgagaa ggattcgaac cttcgacctg ccccttagga
     3061 gggggccgcg ctatccagct gtgccatcgg ggcggatgct ccgctcggcg aagcccagca
     3121 aagataccgc accagctgct gcgaacgcta cgggctcgac taggcgttca gtcacccacc
     3181 agcatgatca tgattccagc ggccacggcc gatccgatca cgcccgccac gttcgggccc
     3241 atcgcgtgca tgagcagata attgtggcga ttggcttcga gcccgacccg gttggcgacc
     3301 cgcgccgcca tggggaccgc cgagacgccg gcagcgccga tcagcgggtt gatgtgctcg
     3361 cgggtcacca ggttcagcag cttgcccatc agaacgccgg acgcggttcc cagcgcaaac
     3421 gcggccatac cgagtgccag gatgcccagc gtgctgggca ccaggaactg ctctgcgctt
     3481 agcttggagc ccacggcaag gccgaggaag atggtcacga tgttgatcag ggcgttctgg
     3541 gtggtatccg acaaccgatc caccacgccg cattcgcgca tcaggttacc gaagcagaac
     3601 atccccagca gaggcgcggc agatggcaac agcagcgcca ccagcagcag cagcagcagc
     3661 ggaaaaacga tgcgctcgag cctggagacc tcgcgcagct gtaccatttc gatcttgcgc
     3721 tccgcctcgg tggtcagggc gcgcatgatg ggcggctgga tcagcggcac cagggccatg
     3781 taggagtaag cggccacggc gattgctccg agcagctccg gggcgagcct gcccgcgacg
     3841 tatatcgccg taggcccgtc ggcgccgccg atgatcccga tggcggcggc ctcgcgcagc
     3901 ccgaagtcga agacgccgag ctccgagagg cccaacgcgc ccaggagggt ggcaaaaatg
     3961 ccgaactggg ccgcggcgcc gagaaagagc gttttcgggt tggccaggag cgggccgaag
     4021 tcggtgagcg ccccgacgcc catgaagatg atcagcggaa aagcgcccga ctcgatcccc
     4081 accacgtagg ccaggtgcag cagaccgtcg ccttcggcga ttcccgcgcc gggaatgttg
     4141 gccagaatcc caccgaatcc gatggtcacg agcagcagag gctcgaactg acgggcaatg
     4201 gccaggtaaa gcagcacgag gcccacgccg atcatgatgg cctgacccca ggtgagctgg
     4261 tttaagcccg aaccctccca gatctgactc agcaggtcca cgatcaggcc agcgtcacca
     4321 acggatcacc caccgcaacc gcatctccct cgcggacgtg aacttcagcc acggtgccgt
     4381 cctgagaggc cgctacggcc gtctccatct tcatggcctc caccaccagg atcgtctgcc
     4441 cctcggtgac cacgtccccc ggctgcacga gcaccttgaa gacgttgcct gcaagcgctg
     4501 cgatgatcgt accggcacct gcccgaacgg gcgccttgaa ggccggttca gccggcttga
     4561 cg
//
LOCUS       DBNHLM010000055         3106 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_665631, whole genome shotgun sequence.
ACCESSION   DBNHLM010000055 DBNHLM010000000
VERSION     DBNHLM010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_665631"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2766
                     /locus_tag="AC16QF_02825"
     CDS             <1..2766
                     /locus_tag="AC16QF_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6447515.1"
                     /translation="LRKAVKEMADLNEIAIIVNSTLDLDKVKDSIYQGLQNLFAFEQM
                     GVFLADYDEEKLYLRLQAGAPFDPELSRILVEEGLPLDSSHSFAARSVVKNRSVFLGA
                     ITDEGLAMAGVGDRLIYEHNPMKSILMCPLEIQNRAIGCIYFSASREAFELHQNDIES
                     IERYVTQLGTALRNAQLFQEAEEARAEAEAANETKGTFLANMSHEIRTPMNAIIGLTG
                     LCLETELDDKQEDYLRKVDGAANALRSIIDDILDFSKLEAGKFEFEDIPFSLNDVLDN
                     LATICMVRCQDKHLELVFQRDPGLPDALSGDPTRLGQILINLAGNAIKFTEEGQIVIE
                     ARETGRVGDRVSIRFDVRDSGIGMNEEQLSRLFQSFSQADATISRQYGGTGLGLAISQ
                     QLSEGLGGHIEVTSEPGVGSSFHFTLEFAVVEAREEGAEREDAPRNLNVLVVDDNEPS
                     RDILREYLESFGYTVTLAESGEEGLALMKPAHDFDLVLLDWMMPGMTGLDVALAIREQ
                     PSPPKVILLSSWNMPSTEHQSMVDAFMAKPVKPSTLLDTIMLAYGKQVVRRKRGLGRG
                     MGHEDLAPVRGARVLVVDDSDINLQIACELLQKVPFVLDTASDGAQAVDKVLAHHYDC
                     VLMDIQMPGMDGYTATGIIRERFSFDELPILAMTANVMAEDRARTRDAGMNGHVAKPV
                     DPAELFQALAEAIPVADYSANLPEAEDATVVEAAAAPQNEPLPDSLPGLELKQGLARL
                     GGNEDLLLTLLRNLLDEYADCPSDLQRLLETGDEEEARKVAHKLRGIANNLGATGIGK
                     CAEDIERCVLDGRRVDPVHVADLQEAQQVLATQLAELLASRTTGGGVGNAAEDVDVGA
                     VLSALKSAIAAFDPGAVELVDQLLAGCDPQSNVVQLLEASRQHLDNFNFTDAEPLVAE
                     AEAALLA"
     gene            2799..>3106
                     /locus_tag="AC16QF_02830"
     CDS             2799..>3106
                     /locus_tag="AC16QF_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447516.1"
                     /translation="MLVLLSAGNGLAASLEFDDLGEPRSITGEWLYMTGDDPGWADPG
                     LEDGGWLPVTVPGPGIGDHPDAQGMVWYRVHVRFGPPIAADLDARAALGVAIGSVESA
                     "
BASE COUNT          588 a          962 c          997 g          559 t
ORIGIN      
        1 ttgcggaagg cggtcaagga aatggctgat ctcaacgaga tcgcgatcat cgtcaactcg
       61 accctcgacc tggacaaggt caaagacagc atctaccagg gcctgcaaaa cctgtttgcc
      121 ttcgagcaga tgggcgtttt cctggcggac tacgatgagg aaaagctgta cctgcgcttg
      181 caggcgggtg ccccgtttga tcccgagctg tctcggatcc ttgtcgagga aggcctgccg
      241 ctcgattcga gtcacagctt tgcagcccga tccgtggtga agaacaggtc ggtctttctc
      301 ggcgcgataa ctgacgaggg cctggccatg gctggtgttg gggatcggct catctacgag
      361 cacaacccca tgaagtccat cctgatgtgt cccctcgaga tccagaaccg ggccatcggc
      421 tgtatctact tctccgccag cagagaagcg ttcgagctgc accagaacga catcgagtcc
      481 atcgagcgct acgtgaccca gctgggcacg gctctgcgca acgcccagct ctttcaggaa
      541 gccgaagaag cccgcgctga ggccgaggcc gccaacgaga ccaaggggac attcctggcc
      601 aacatgagcc acgaaatccg cacgcccatg aacgccatca tcggcctgac cggtctctgc
      661 ctggaaaccg agctcgacga caagcaggag gactacctga gaaaggtcga cggtgcggca
      721 aacgccctgc gcagcatcat cgacgacatt ctcgacttct ccaagctgga agcgggcaag
      781 ttcgagttcg aggacattcc cttctcgctc aacgacgtgc tcgacaacct cgccaccatc
      841 tgcatggtgc gctgccagga caagcatctg gagctcgtct tccagcgcga tcccgggttg
      901 cccgacgctt tgtcgggtga tccgacccgt ctcggtcaga ttctcatcaa cctggccggc
      961 aatgccatca agttcaccga ggagggccag atcgtcatcg aggcccgcga gaccggccgc
     1021 gttggcgaca gggtgagcat tcgcttcgac gtgcgtgaca gcggcatcgg catgaacgag
     1081 gagcagctga gcaggctgtt ccagtctttt tcacaggccg atgccactat cagtcgtcag
     1141 tatggcggca ctggccttgg ccttgccatc tcgcagcagc tcagcgaagg ccttggcggt
     1201 cacatcgagg tcacgagcga gcccggtgtc ggcagcagct ttcatttcac tctcgagttc
     1261 gccgtcgtcg aggcgcgcga agaaggggcc gagcgggagg atgcgcccag gaacctcaac
     1321 gtgctggtgg tggatgacaa cgagccgtcc cgggacattc taagagagta tctggaatcg
     1381 ttcggctaca cggtgacctt ggcggagagc ggagaagagg gcctcgccct catgaaaccc
     1441 gcgcacgact tcgatctggt gttgctggac tggatgatgc ctggcatgac gggcctggac
     1501 gtggccctcg cgatccgcga gcagccgagc ccgccaaagg tcatcctgct gtcgtcctgg
     1561 aacatgccga gtaccgagca tcagtccatg gttgatgcgt tcatggcgaa accggtgaaa
     1621 ccgtcgaccc tgctcgacac catcatgctc gcctacggca agcaggtggt aaggcgcaaa
     1681 cgcggcctgg gtcgggggat gggccatgaa gacctggctc ccgttcgcgg cgcacgggtg
     1741 ctcgtggtgg atgattccga catcaacctc cagattgcct gcgaactgct gcagaaggtg
     1801 cccttcgtgc tcgataccgc cagcgatggc gcgcaggcgg tggacaaggt gctggcccac
     1861 cactacgact gcgtgctcat ggatatccag atgcccggaa tggacggcta caccgccacg
     1921 ggcatcattc gcgagcgttt ctcgttcgac gagctgccga tcctcgccat gaccgccaac
     1981 gtgatggccg aggaccgggc gcgcacccgc gatgccggca tgaatggcca tgtggccaag
     2041 ccagtggacc ctgccgagtt gttccaggcg ctggccgagg ccatcccggt agccgattac
     2101 tccgccaacc ttcccgaggc agaggacgcc actgtcgtgg aggcggcggc tgctccgcag
     2161 aacgagccgc tgccggattc cctgcccgga ctcgagctca agcaaggact tgcccggctt
     2221 ggaggcaacg aagacctgct gctgacgctc ctgcggaacc tgctggatga gtacgccgat
     2281 tgcccttccg acctgcagcg tttgctggaa accggagatg aagaagaggc gcgcaaggtc
     2341 gcacataagc tcaggggcat agcaaataac ctcggggcca cgggcatcgg gaaatgcgcc
     2401 gaggacatcg agcgctgtgt cctcgacggg cggcgggtgg atccggtcca tgtggcggac
     2461 ctccaggaag cccagcaggt cctggcgacg caactcgctg agctgctggc gtcgcgaacg
     2521 acgggcggtg gcgtggggaa cgcagccgaa gatgtcgacg ttggggccgt gctgtccgcc
     2581 ctgaagtccg caattgccgc tttcgacccc ggtgctgtag agctcgtcga tcagctgctc
     2641 gcgggatgcg acccgcagag taacgtcgtg cagctgctcg aagcgtcccg tcagcacctc
     2701 gacaacttca acttcactga cgcggagccg ctggtggcgg aagcggaggc ggccctgttg
     2761 gcctgatcat gaagctgcct cgacggtgct gcgccctcgt gcttgttttg ctctccgccg
     2821 gcaacggtct ggccgcttcg ctggaattcg acgatctcgg tgagccgcgc tcgataaccg
     2881 gtgagtggct ttacatgacg ggcgatgacc ctggttgggc tgatccgggc ctggaggacg
     2941 gcgggtggct gccggtgacc gtgccaggtc ctggaatcgg cgaccacccc gacgcgcagg
     3001 gaatggtctg gtaccgggtg cacgtccgtt tcggtccgcc gattgccgcc gatctcgacg
     3061 cccgcgcggc gctcggggtc gccattggca gcgtggagag cgccta
//
LOCUS       DBNHLM010000056         3928 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1084673, whole genome shotgun sequence.
ACCESSION   DBNHLM010000056 DBNHLM010000000
VERSION     DBNHLM010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1084673"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..2130)
                     /locus_tag="AC16QF_02835"
     CDS             complement(28..2130)
                     /locus_tag="AC16QF_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR6447517.1"
                     /translation="MSRRDATYRLAVDIGGTFTDVVYEPDAAGAQRFTVKVLTTYDDP
                     ANGVMQGIEAVLERAGAEAGDAGLVLHGTTLATNALIERRGARTALLTTEGHRDALEM
                     ALENRFEQYDVNIDRPRPLVPRRLRLPVRERLDAAGRVLVPMDSKSLDAAVAALDGHG
                     VESVAIGFLHAYANPDHEHAAAERVREKLPDVSITLASQVCPEIREYERLSTACANAY
                     VKPLMARYLGSLQRKLTDRGMVCPVLLMTSGGGLTTLETAAEFPIRLVESGPAGGALL
                     AARIAAEGGHERVLSFDMGGTTAKICLIDGAAPLLSRSFEVDRTYRFKKGSGLPVRIP
                     VIEMVEIGAGGGSVARVDKLSRITVGPDSAGSEPGPACYGRGGRDATVTDGDVVMGRL
                     DPKSFAGGRLALDTAAAERAVTEAIGDPLGLNLHTAAFGISEVVDENMAAAARAHAVE
                     WGEDTQGRTLVAFGGAAPLHAAQLAVKLKLDRVIVPAHAGVGSAIGFLIAPVSYEVVR
                     SRYMRVSGFDADLVNAVMAEMRAEALEVVSSAAETEGLLETRRAYMRYVGQGYEIAVP
                     VPGGTIAEDAGEVLRAAFDHEYRRLYRRTIPNLDVEVLSWSLALAAPQPEFSADSAAP
                     RTDPAEAVRRVSLFEPLTGEMADGAFYRRVDIRPGHWLEGPAVISEEQTTTVVPAGFS
                     AGVTVRGHLVLERMETET"
     gene            complement(2132..3322)
                     /locus_tag="AC16QF_02840"
     CDS             complement(2132..3322)
                     /locus_tag="AC16QF_02840"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6447518.1"
                     /translation="MHHYLLGTASWFTAFGIQGVMFAWLVTMVLRESPEMVGLAQMTL
                     LLPGTLLMLIGGTYADRFGGRRIVIGAQSVAACAPLLLLVLIWSDMLTYASMLVYAVF
                     MGCATAFVTPARDGLLNEIAEGRVQRAVMLASMIQFGGQMIGFTLAAGADLVGPLVVL
                     GAQSLCLALGVLAFVLIPRPHEGRHVRTGRLLASLAEGARTVFSSASMRLIVVQNVAM
                     AIFFMGSYVVAMPLLVREVFNGTARDLAFTNGSNSLGLVLSIVLMLRLGDVTRQGRAL
                     VLSQLAGGLMLIAAALMTSFALYVLMLFIWGACGGIAMTMARTIMQEQAPDAQRSRVM
                     SFYALSFMGAGPLGALFTGYMVELLGPQQALVLSGSLMAVVACILAVGSNLWSLRAYQ
                     VASA"
     gene            3559..>3928
                     /locus_tag="AC16QF_02845"
     CDS             3559..>3928
                     /locus_tag="AC16QF_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR6447519.1"
                     /translation="MQDIIEELQARRAAADLGGGEHRIEAQHGKGKLTARERISLLLD
                     EGSFEEWDKFVEHRCTDFSMEEQKIPGDGVVTGYGTVNGRLVFVFSQDFTVFGGALSE
                     AHAEKICKVMDQAMKVGAPLI"
BASE COUNT          739 a         1374 c         1254 g          561 t
ORIGIN      
        1 gggtccgcag ttcactcacg ttcgcactca cgtctcggtc tccatccgtt ccagcaccag
       61 gtgacccctg accgtgaccc ctgccgagaa cccggcgggc accacggtgg tcgtctgctc
      121 ttcggagatc acggcgggtc cctccagcca gtggccgggc cggatgtcga cccgtcgata
      181 gaaggcgccg tcggccattt cccccgtcag cggttcgaac agggagaccc tgcgcacggc
      241 ctctgcaggg tccgttcgcg gggccgcgga gtcggccgaa aactccggct ggggcgcggc
      301 gagagcaagc gaccagctca ggacctcgac atccagattg ggaatggtgc gccggtacag
      361 gcgccggtat tcgtggtcga aggccgctcg gaggacctcg cccgcgtcct cggcgatcgt
      421 cccgcccggt acgggcacag cgatctcgta gccctggccc acgtagcgca tgtaagcgcg
      481 acgggtttcc agcagccctt ccgtctctgc tgcggaggac accacctcca gggcttcggc
      541 acgcatctcc gccatgacgg cgttgaccag gtccgcatcg aaacccgata cgcgcatgta
      601 acggctgcgc accacctcgt agctgaccgg agcgatcagg aaaccgatcg ccgaccccac
      661 acccgcgtgg gccggcacga tcacccgatc cagcttcagc ttgacggcga gctgggcggc
      721 atggagcggt gccgctccgc caaaggcgac aagggtccgt ccctgggtat cttcgcccca
      781 ctccaccgca tgggcccggg ccgcagccgc catgttctcg tcgaccacct cgctgattcc
      841 gaacgcggcc gtgtgcaggt tgagcccgag cgggtcgccg atcgcttccg tcacggcgcg
      901 ctcggccgcg gcggtatcca gggcgaggcg cccgccggcg aaggacttcg gatccagacg
      961 gcccatgacg acgtcgccgt cggtgaccgt cgcgtcgcgc ccgccacgcc cgtagcaggc
     1021 gggtccaggc tcggaccccg cgctgtccgg gcccaccgtg atgcgcgaca gcttgtctac
     1081 ccgggcaacc gagccgccgc cggcgccgat ctcgaccatc tcgatgacgg gaattcgaac
     1141 cggcagcccg cttcccttct tgaagcggta agtccgatcc acttcgaagg accgggaaag
     1201 cagcggcgcg gcgccgtcga tcagacagat cttggccgta gttccgccca tatcgaagga
     1261 cagcacccgt tcatggccac cctcagccgc gattcgggcg gcgagcagcg ccccgcctgc
     1321 gggtcccgac tccaccaggc ggatgggaaa ctccgccgcc gtctccaggg tggtgagccc
     1381 accgcccgac gtcatcagga ggacggggca gaccatcccg cgatcagtga gcttacgctg
     1441 cagactgccc agatagcggg ccatgagcgg cttgacgtag gcgttggcgc acgccgtcga
     1501 caacctctcg tactcgcgga tctcgggaca gacctgagag gccagcgtga tcgagacgtc
     1561 gggcagcttc tcgcgaacgc gctcggccgc ggcgtgctcg tgatcagggt tcgcgtaggc
     1621 gtgcaggaaa ccgatcgcca cgctctcgac cccgtgcccg tccagggcgg ccaccgcagc
     1681 gtccagagac tttgaatcca tcggtaccag caccctgcca gcggcgtcca gacgttcccg
     1741 gacgggcagg cgcaagcgcc ggggaaccag gggccgcggc cggtcgatgt tcacgtcgta
     1801 ctgctcgaaa cgattctcca gcgccatctc cagggcatcg cgatgaccct cggtggtcag
     1861 aagcgccgtt cgcgcgcctc ggcgctcgat cagcgcgttg gtcgccagcg tggtgccatg
     1921 aagaacgagc cccgcgtcgc ccgcctcggc gccggcgcgt tccagcacgg cttcaatccc
     1981 ctgcatcacg ccgttggcag gatcgtcgta ggtcgtcagc accttgacgg tgaagcgctg
     2041 cgcacccgcc gcatcgggct cgtaaaccac gtcggtgaac gtaccaccga tatccacggc
     2101 caaccggtac gttgcgtccc ttctgctcat gtcaggcaga cgccacctga taagctctca
     2161 gagaccagag atttgagccc acggcgagaa tgcaggccac cacggccatc aggctgcccg
     2221 acaggacgag cgcctgctgg gggccgagca gctccaccat gtagcccgtg aagagcgctc
     2281 ccaggggccc ggcccccatg aacgagagcg cgtaaaagct catcacccgg ctgcgctggg
     2341 cgtcaggcgc ctgctcctgc atgatggttc ttgccatggt catcgctatt ccgccacagg
     2401 cgccccagat gaacaacatg agcacgtaaa gagcaaagct cgtcatcagc gccgcggcga
     2461 tcagcatgag gccacctgcc agctgcgaca gcaccagcgc ccggccctga cgggtcacat
     2521 cgccaagccg gagcatgagg acgatgctga gcaccagccc gagagagttc gagccgttgg
     2581 taaaggccag gtcccgggcg gtgccgttga acacctcccg gaccagcaac ggcatggcca
     2641 ccacgtaaga ccccatgaag aagatggcca tcgccacgtt ctgcaccacg atcaaccgca
     2701 tcgacgcaga cgagaagacg gtccgggcac cctcggccag ggaagccaac aaccggcccg
     2761 tgcgcacgtg ccgcccctcg tggggacgcg gaatcagcac gaatgccagc acgccgagcg
     2821 ccaggcagag gctctgagcg ccgagcacca ccaggggacc caccaggtcg gcgcccgcag
     2881 cgagcgtgaa gccgatcatc tgcccgccga actggatcat gctggccagc atgaccgcgc
     2941 gctggaccct gccctccgcg atttcgttga gcagaccgtc gcgggccggg gtgacgaaag
     3001 cggtggcgca acccatgaac accgcgtaga ccagcatgct ggcgtaagtg agcatatcgc
     3061 tccagatcag gacgagcaga agcagcggcg cgcaggccgc cacggattgc gcgccgatga
     3121 cgatgcgccg gcctccgaag cggtccgcat aagtgccccc gatcagcatc aggagggtcc
     3181 ctgggagcag cagggtcatc tgagccagac caaccatctc cggagattcg cgcagcacca
     3241 tcgtgaccag ccacgcgaac atgacgccct ggataccgaa cgcggtaaac cagctcgcgg
     3301 tcccgagcag gtaatgatgc atgggcttca agatatcgtt gcggcgaaaa ccgctccgag
     3361 caggaaacga cgccgcagta taccgcctcg aggggctggc cggaccgccc ctgaagcgcc
     3421 cggcacggcg cgcgcaagat gtggtcaccg cgaacgcggc aggatatcct cgcggtttcg
     3481 tctcggacaa caacaaccat atccctccgg gagcatccgc acccggtccg ggggggcatc
     3541 ccacggcagg agagccagat gcaggacatc atcgaggagc tgcaggcgcg acgcgcggca
     3601 gccgatctcg gcggcggcga gcaccgcatc gaagcacagc acggcaaggg caagctcaca
     3661 gcccgcgaac gcatttccct cctgctcgac gagggcagct tcgaggaatg ggacaagttc
     3721 gtggagcacc gctgcaccga cttctccatg gaggagcaga agatccccgg agacggcgtc
     3781 gtcaccggct acggcacggt gaacgggcgg ctggtgttcg tcttcagcca ggacttcaca
     3841 gtgttcggcg gcgccctgtc cgaagcccat gccgagaaga tctgcaaggt gatggaccag
     3901 gccatgaagg tcggcgcgcc tctgattg
//
LOCUS       DBNHLM010000057        11721 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_665652, whole genome shotgun sequence.
ACCESSION   DBNHLM010000057 DBNHLM010000000
VERSION     DBNHLM010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11721)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11721)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11721
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_665652"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /gene="phoR"
                     /locus_tag="AC16QF_02850"
     CDS             <1..1323
                     /gene="phoR"
                     /locus_tag="AC16QF_02850"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HIR6447520.1"
                     /translation="ISRQEWILGATLFVLAALGGWAFGNFGWAFLIALGIHAAVHRRE
                     RLAFFRWAERPLRRPENRIESWESRVSRVFRTIRRERGRHRAALRQLRAIRTIADALP
                     DAVVVIDRSGDIEMFNDAARDILQLHRRDRGSNLSALVRQPDFVALITGKLADDIVEF
                     ASPFAGEHRLEARRISLVDARSLVLVRDVSQLNRLLSMRQDFIANVSHELRTPLTVIV
                     GYLETLAGEQLDESTTRELIGKLGSPTKRMQALVNDLLLLTRLEASPSPSDDELHAVD
                     MGLIIEGIAIDARALSDGHHRISVRLDCDLRVLGIESELHSACANLLNNAVRYSPEGG
                     DIQVTWENIGPAARFSVNDHGLGIPPEHLSRLTERFYRVDLARSSVRGGTGLGLAIVK
                     HVLKRHGSELQVKSELGEGSTFYCDFPATRLADPLVSNQSGSSSLEKS"
     gene            1320..2282
                     /locus_tag="AC16QF_02855"
     CDS             1320..2282
                     /locus_tag="AC16QF_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR6447521.1"
                     /translation="MRKLLISALVALVLCNWAIASENLDPNLPEYQRVGGVSGNLSST
                     GSDTLANLMTLWTESFKREYPNVNIQVQAAGSSTAPPALTEGTSNFGPMSRLMKDKEI
                     ESFESRFGYKPMAIRVAIDALAVYVHKDNPIETMTISEVDAVFSATRRCGGPQDIGRW
                     GDLGLSRNWVDRPLQLYGRNSVSGTYGYFKQVALCSGDFKNTVNEQPGSASVVQAVAS
                     SLNGIGYSGIGYITSGVKALALSEGEGAEHVAATAENAISGTYPLSRYLYVYVNKKPG
                     TSLAPLELEFLKMVLSKSGQEIVAKDGYIPVSASVVSRELRKLQ"
     gene            2313..4499
                     /locus_tag="AC16QF_02860"
     CDS             2313..4499
                     /locus_tag="AC16QF_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR6447522.1"
                     /translation="MALNADLSHLPTRRRLTNAVTRYVVALGGAAVIGAIALIFFYLL
                     WVAIPIMAGAEIEPGQRLSLPVGTPLLLEANETGEIGLRIDSSGQAIFFSLNDGSIVD
                     RVTLASSVERVKRVLPHSSMYAVQSPQSGIVIYESRHPVTFDNDVRQIRPELNAVFPD
                     TWIDVDPGADFDVHFADFEVLVVAVTGTNLQIQHFRDAEAGFELYEPRRANLSLERVY
                     ERVIIGPRGAWVYLVADDGSVAVLDLQRPTSPRQIFVGKLVAEGSSLNALEPLLGRYS
                     LLAGNDKGQVTQWFMVPGEFGYTLERARTFKLDRAITRLIAEQRRKGFGAVDAEGRFH
                     LLYTTSSRVLADRRLDSNRFSVASIAPRANMLLTGDESGDVTTYRIHNEHPEISWSAL
                     WSKIWYEGYPEPVYSWQSSSADNDFEAKFSLTPLLFGTLKAAFYAMVFAVPIAIMGAI
                     YTAYFMAPAMRRIVKPGVEIMAALPTVILGFLAGLWLAPLIEANLAGVIGSILALPVG
                     ILLTAVLWGRLPEKVTGRFDGWYGIFILPTIILTVVAGFGLGPWIESVFFGGNTRGWF
                     LNELGLAYDQRNALVVGLAMGLAVIPTIFSITEDAIYGVPTHLINGSLALGATPWQTL
                     VRVVILTASPGIFSAVMIGLGRAVGETMIVLMATGNTPVMDMNLFEGMRTFAANIAVE
                     LPESEVGSTHYRILFLTALVLFMITFVFNTAAEVVRQRLRSRYGNL"
     gene            4502..6196
                     /gene="pstA"
                     /locus_tag="AC16QF_02865"
     CDS             4502..6196
                     /gene="pstA"
                     /locus_tag="AC16QF_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR6447523.1"
                     /translation="MIHSDHDSLRAWFRSGELWIWANAAAVGISIIAIVGILILLAAR
                     GFSHFWPADIQLMTYRDDGDRTAVALGEIADSETLSVQQFVESVGGSGRAVPEGLDEV
                     ERLLVKTGNRRYDPPDFTWIFTHKIDAVSFPDDATVFERMEWGNAYGYLRAVGGPQET
                     VSDPALLWDTFGNLMERAAGLRASIEDIERGVLNEINFGMEKVRLERRSIELGGLDVD
                     QAATAMQELDERDLDLEKRYESAEQELRSLYRELQRDYLRVELATGQVIEMRLAEIVR
                     AWRPNQMSALDKLWHYLESFGLFLSEQPREANTEGGIFPAIFGTVLMVLLMSVLVTPF
                     GVIAAVYLREYARQGAFVRLIRIAVNNLAGVPSIVYGVFGLGFFVYFLGGNLDQLFFP
                     EALPAPTFGTPGLMWASLTLALLTVPVVVVSTEEGLTRIPRAIREGSLALGATKAETL
                     FKVVLPMASPSILTGVILAVARAAGEVAPLMLVGVVKLAPTLPLDGNFPYLHLERKFM
                     HLGFHIYDVGFQSPNVEAARPLVYATALLLVLIIIILNLAAIMLRSRLEDRYRGVE"
     gene            6278..7066
                     /gene="pstB"
                     /locus_tag="AC16QF_02870"
     CDS             6278..7066
                     /gene="pstB"
                     /locus_tag="AC16QF_02870"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR6447524.1"
                     /translation="MTDTKQNASGSNGTSLLVDRLSLFYSGQKALHEISLEIPRKQAT
                     AFIGPSGCGKSTLLRCFNRMNDLIDGVETTGRILLDGTDINSKEVDVAQLRRLVGMVF
                     QRPNPFPKTIYENVAYGLRLQDTLPKTEMDERVEWALRSAALWDEAKDRLKESALSLS
                     GGQQQRLVIARAIAIKPSVLLLDEPASALDPISTLKIEELIHQLKTEYTIVIVTHNMQ
                     QAARVSDYTAFLYLGELIEFGKTDTLFTNPKERQTEDYITGRYG"
     gene            7101..7808
                     /gene="phoU"
                     /locus_tag="AC16QF_02875"
     CDS             7101..7808
                     /gene="phoU"
                     /locus_tag="AC16QF_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR6447525.1"
                     /translation="MTPHPHISEQFDHELDQARNRLMEMGGKVEQQLKNACKALTNHD
                     LELAETIKTGDRSINQLEVDLDELCVQIIARRQPAASDLRTLVSVMKASTDLERIGDE
                     AKRIAKMAIAISRLTYPRDQYTDIRKLVALVMAQVSATLDAFARLDVDKAIGLIRYDA
                     EIDDAYDAIVRDRAVQMKEMANNPEGIEQTLHVLWAARALERIGDHAKNISEVVVFLV
                     RGEDIRDRALAAKLTGG"
     gene            complement(7818..8603)
                     /locus_tag="AC16QF_02880"
     CDS             complement(7818..8603)
                     /locus_tag="AC16QF_02880"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6447526.1"
                     /translation="MDLQEGVAIVTGSSSGVGAATARQLAERGAHVVINYASNEDGAR
                     ATQEACEAFGVETAVVRANVADDADCRRLAATAEERWGRIDALVNNAGTTKFCAHHDL
                     EGLDSDDFMNIYGVNLVGPYQMTRAVAPAMKRGGRGSIVNVASIAGVTGIGSSIAYAA
                     SKGALVTMGLSLARVLGPEIRVNTVCPGFIQGDWLEQGMGAEIYARTKKFLEESTPLR
                     LTTTPDTIAEAIVYFLSGADVVTGETLILDGGHHLNQMPFARR"
     gene            8723..9373
                     /locus_tag="AC16QF_02885"
     CDS             8723..9373
                     /locus_tag="AC16QF_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR6447527.1"
                     /translation="MDQMTISAILWDFGGVLTTSPFEAFNRFEAEHGIPADFIRRINA
                     TNPDGNAWAQFESSAIGLNEFDEAFARESAAQGHRIPGRKVLELLSGELRPRMVEALH
                     SCKVSYQVACITNNVKSGQGPAMASNRARAAAMEGVMALFDLVVESSIEGIRKPDPRI
                     YELTCERLGVRPEQAVFLDDLGVNLKPARALGMHTIKVVEESQALEELGSLTGLAL"
     gene            complement(9383..9625)
                     /locus_tag="AC16QF_02890"
     CDS             complement(9383..9625)
                     /locus_tag="AC16QF_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin family protein"
                     /protein_id="HIR6447528.1"
                     /translation="MARVVFPEHLLQHTGGEREIEVPAADFRELLINLERRWPEAGAV
                     LEKCAVAIDGQIYQDAFLEPLEPDSEVFFMQRIEGG"
     gene            complement(9679..>11721)
                     /locus_tag="AC16QF_02895"
     CDS             complement(9679..>11721)
                     /locus_tag="AC16QF_02895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6447529.1"
                     /translation="VSGADFPEAKHGEIGGEGGGDVADLAKNVMARDKVLYHGHAVAA
                     VAAKSLSVADAALEAIEVEYEVLTPVLDLLEAMKEDAPLLDDHCFTKNLPETPDKPSN
                     IAAVMQLARGDVDEGFAQADVIVEGEYNIPMAHQGYIEPHACTATVNEGGQATIWCST
                     QGHFEFRSMTAKMLNMEISDLKFIASEIGGGFGGKTVVYLEPLAVLLARKSRRPVKMM
                     MTREEVFRATGPTSGTHCRIKVGAKRDGTITSATAWLAYEAGAFAGAPMGPGAMSIFA
                     PYDLENFKIEAFDVLLNKPKVAAYRAPGAPQSMHAMEVALDDLALELGMDPIDLRMKN
                     AAGEGTQAPYGPKFPQIGFKECLEAAKAHPNYTKPLPEGAGRGVAAGFWFNVGMQSSA
                     EVHVNEDGTVTVIEGNPDIGGSRASMCMMASETLGVPYEKIRALVGDTESTGFSNVTG
                     GSRTTFATGMAVVQACEDIITQCKARAAATWGMDADQVDWKDGQAVPAPGVNADVTPL
                     SLADIAAKAGRTGGPLLGRASLNAQGAGASFSVNICDLQVDPETGKVDVLGFTAIQDA
                     GKAIHPSYVEGQMQGGAAQGIGWALNEEYIFDENGVMENAGFLDYRIPVASDLPMIDC
                     HIVEVPNPSHPFGVRGVGETPIVAPLAATANAFRAATGVRVNDLPLSPPRVLSALD"
BASE COUNT         2204 a         3829 c         3489 g         2199 t
ORIGIN      
        1 atctcccgcc aggaatggat cctcggcgcc acgcttttcg tcctggccgc cctcggcgga
       61 tgggcgttcg ggaacttcgg ctgggccttc ctgatcgcgc tcgggattca tgccgcggtc
      121 caccgccgcg agcgccttgc cttctttcga tgggccgagc ggcccctgcg acgccccgag
      181 aaccgaatcg agtcatggga gtcgagagtc tcccgggtct ttcggacgat tcgacgggag
      241 cggggtcgtc atcgagccgc cttgcgccag ctgagagcca ttcgcaccat cgccgatgcc
      301 ctgccggacg ccgtggtcgt gatcgaccga tccggcgaca tcgagatgtt caacgacgcc
      361 gccagggaca tcctgcagct gcaccgacgc gaccgcggct caaacctctc cgccctcgtg
      421 cggcaacccg acttcgtagc cctcatcacc gggaagcttg ccgacgatat cgtggagttc
      481 gcgtccccgt tcgccggcga gcaccgtttg gaggctcgcc ggatatcgct ggtggacgcg
      541 cgctccctag tgctggtcag agacgtctcg cagctcaacc ggctgctgag catgcgccag
      601 gacttcatcg cgaacgtttc ccacgagttg cgaacaccgc tgacggtgat cgtcggctac
      661 ctcgagacgc tggccggcga gcagttggac gaatcaacca cccgggagct gatcggcaag
      721 ctcggctccc cgacgaaacg catgcaggcc ctggtcaacg atctcctgct gctcacccgg
      781 ctggaggcta gcccgtcccc gtcggacgac gagcttcacg ccgtggacat gggcctcatc
      841 atcgaaggga ttgcgatcga cgcgcgagca ctgagcgatg ggcatcaccg gatttccgtc
      901 cggctggact gcgacctgcg cgtcctgggc atcgaaagcg agctgcacag tgcctgcgcg
      961 aacctcctaa acaacgccgt tcgctacagc cccgaaggcg gcgacatcca ggtcacctgg
     1021 gagaacatcg gacccgccgc gaggttctcg gtcaacgatc acgggctcgg cattcctcca
     1081 gagcacctgt cccggctcac cgagcgtttc tatcgggtcg atctcgcccg atcaagtgtg
     1141 cgcggcggca ccggcttggg gctagccatc gtcaagcacg tcctcaaacg ccacggcagc
     1201 gagctgcagg taaagagcga actcggcgaa ggaagcacgt tctactgcga ctttcccgcc
     1261 actcgattgg ccgacccgct cgtttccaat caatccggat catcctcctt ggagaaatca
     1321 tgagaaagct actgatctcg gctctggtcg ccctggtgct ctgcaactgg gccatcgcga
     1381 gcgaaaatct cgatccgaac ctgccggaat accagcgggt aggtggcgtc tcgggcaacc
     1441 tgtcgagcac cggctcggac accctcgcaa acctgatgac cctttggacg gaatccttca
     1501 aacgcgagta ccccaacgtc aacatccagg tacaggcagc cgggtcgtct accgctcctc
     1561 ccgccctgac cgaaggcacc tccaacttcg ggccgatgag ccggctcatg aaggacaagg
     1621 agatcgagtc gttcgaatcc cgctttggtt acaagccaat ggccatccgg gtcgccattg
     1681 acgcgctggc cgtctacgtc cacaaggaca atcccatcga gaccatgacc atcagcgagg
     1741 tagacgccgt tttctccgcc acgcgccgat gtggtggacc tcaggacatc ggccggtggg
     1801 gcgacctcgg cttgagccgg aattgggttg accggcccct gcagctgtat ggccgcaatt
     1861 cggtgtccgg cacctacggc tacttcaaac aagtcgcgct gtgctcggga gacttcaaga
     1921 acacggtcaa cgagcagcca ggatccgcgt ctgtcgtcca ggccgtggcg tcgtcgctga
     1981 acggcatcgg ctattccggg atcggctaca tcacctcagg cgtcaaagcg ctggccctca
     2041 gcgagggtga gggagccgag cacgtcgccg cgactgcgga aaacgccatc agcgggacct
     2101 atccgctgtc acgttacctg tatgtgtacg tgaacaagaa gcccggcacg tctttggctc
     2161 cgctcgagct cgaattcctc aagatggtgc tgtcgaaatc gggtcaggaa atcgttgcca
     2221 aagacggcta tattccggtc tcggcgagcg tcgtcagccg cgagctcaga aaacttcaat
     2281 aagcgcccac cgagcgaatc gaagaacccg caatggcctt gaacgccgat ctgtcgcacc
     2341 tgcctacccg caggcgtctg accaatgccg tcacgagata cgtggtggcc cttggcggcg
     2401 cggcggtcat cggcgccatt gcgctgatct ttttttacct gttgtgggtg gccatcccga
     2461 tcatggcagg cgcggagatc gagcctggac agcggctgtc tctccccgtc ggcacgccgc
     2521 tactgctcga ggccaacgag acgggcgaga tcggtctgcg catcgactcg tcgggacagg
     2581 cgatcttctt ttcgctcaac gatggcagca tcgtcgaccg ggtcaccctg gccagttccg
     2641 tggaacgggt gaaaagggtc ctgccccact cctccatgta cgccgtgcag agcccgcaga
     2701 gcggtatcgt catctacgaa tccagacatc cggtgacgtt cgacaatgac gtgaggcaga
     2761 tccggcccga gctcaacgcg gtatttccgg atacctggat cgacgtcgac cccggcgcag
     2821 atttcgacgt ccacttcgcc gacttcgaag ttctcgtggt cgccgtgacc ggaacgaacc
     2881 ttcagattca gcactttcgg gacgcggagg cgggtttcga gctctacgag ccccgacggg
     2941 ccaacctctc cctcgagcgg gtctacgaac gcgtcatcat cgggccgcgc ggcgcgtggg
     3001 tttatctggt cgccgatgac ggatccgtcg ccgtgctcga tctccaacgc ccaacctcgc
     3061 cgcggcagat cttcgtgggc aagctggtcg ccgaaggctc gtcgctgaat gcgctcgaac
     3121 ccctgctcgg acgctattct ctcctggcgg ggaacgacaa gggccaggtg acccagtggt
     3181 tcatggttcc cggcgagttc ggttacaccc tggagcgcgc tcgaaccttc aaactcgacc
     3241 gcgcgatcac gaggctgata gccgagcagc gccgcaaagg cttcggagcg gtggacgccg
     3301 aggggcgctt ccacctcctt tacacaacct catctcgcgt gctggcggat cgtcggctcg
     3361 actcgaaccg cttctccgtc gcctccatcg cgccccgtgc gaatatgctg ctcaccggtg
     3421 acgaaagcgg cgacgtcacg acctaccgga ttcacaacga acacccggag atctcctgga
     3481 gcgcgctgtg gtcgaagatc tggtacgagg gatatccgga acccgtttac agctggcaat
     3541 catcatcggc cgacaatgac ttcgaggcga agttctccct gacgccgctg cttttcggga
     3601 cgctgaaggc ggccttttac gccatggtgt tcgccgtgcc gatcgccatc atgggggcga
     3661 tctacaccgc atacttcatg gcacctgcca tgcgccgtat cgtcaaaccc ggcgtagaga
     3721 tcatggccgc gttacccacc gtgatcctcg gatttctggc cggcctatgg cttgcgccac
     3781 tcatcgaggc caacctcgcc ggcgtcatcg gcagcatcct ggcgctgccg gtggggatcc
     3841 tgctgacagc cgtcctctgg ggccgtctgc cagagaaggt gaccggacgg tttgatggct
     3901 ggtacggaat tttcatcctg ccaacgatca tcctcaccgt ggtcgcgggc tttggcctcg
     3961 gcccgtggat cgagagcgtc tttttcggcg gcaacaccag aggctggttt ctgaacgagc
     4021 tgggcctcgc ctacgaccag cgcaatgccc tggtcgtcgg cctggcgatg ggactcgccg
     4081 tgatacccac gattttctcg attaccgaag atgccattta cggtgtgccc acccacctga
     4141 tcaacggatc actcgctctg ggagccaccc catggcagac cctggtccgc gtggtcatcc
     4201 tcactgcgag tccggggatt ttctccgcgg tgatgatcgg gctcggccgc gccgtcggcg
     4261 aaaccatgat cgttctcatg gccaccggaa acacgccggt tatggacatg aacctcttcg
     4321 aaggtatgcg gacttttgca gcaaacatcg ccgtggagct gccggaatcg gaggtcggca
     4381 gcactcacta tcggatcctc ttcctaaccg cgctggtcct gttcatgatc accttcgtgt
     4441 tcaacacggc tgccgaggtc gtccgtcagc gtctgcgatc acgctacggg aacctgtgac
     4501 gatgattcac tcggaccacg acagccttcg cgcctggttc cgttcaggcg agctatggat
     4561 ctgggcgaat gccgcagcgg ttggtatcag catcattgcc atcgtcggca tactgatact
     4621 cctggcggca cggggcttct cgcacttctg gcccgccgac attcagctga tgacctatcg
     4681 ggacgacggc gaccgcacgg cggtggcact gggggagatc gcggactccg aaaccctgtc
     4741 cgttcagcag ttcgtagagt ccgtgggcgg atccggacga gccgtccccg aaggtctcga
     4801 cgaggtagag cggctcctcg tgaagaccgg caaccgtcga tacgaccctc cggacttcac
     4861 ctggatcttc acccacaaga tcgacgccgt cagctttccg gatgacgcca ccgtcttcga
     4921 gcgcatggag tggggaaacg catacggata tctgagagcc gtcggcggcc cacaggagac
     4981 cgtctcggat ccggcgctgc tctgggacac gttcggcaac ctgatggagc gcgccgctgg
     5041 tctgcgcgca tccatcgagg acatcgagcg gggcgtgctc aacgagatca atttcggcat
     5101 ggagaaggtt cgcctggaac gccggagtat cgagctgggc ggcctggacg tcgaccaagc
     5161 ggccaccgcc atgcaggaac tcgacgagcg cgatcttgat ttagagaagc gttacgagag
     5221 cgcagagcag gaactacgtt cgctctaccg ggaactccag cgcgattacc tccgggttga
     5281 gctggcaacc ggacaggtca tcgagatgag gctggccgag atcgtccgcg cctggcggcc
     5341 gaaccagatg tctgcgctgg acaaactttg gcactacctg gaaagcttcg gcctctttct
     5401 atctgagcag cctcgcgaag ccaacaccga gggcggcatc tttcccgcca ttttcggtac
     5461 cgttctgatg gtgctgctca tgtccgtcct ggtcacgcct ttcggcgtca tcgcggccgt
     5521 ctacctcaga gagtacgccc ggcagggcgc attcgtccgg ctcatccgga ttgccgtcaa
     5581 caaccttgcc ggggtgcctt cgatcgttta cggcgtgttc ggcctgggat tcttcgtcta
     5641 ctttctcggc ggcaacctcg accagctgtt ctttcccgaa gcgctcccgg cgcccacctt
     5701 cggaacaccg ggcctgatgt gggcttcgct cacgctcgcc ctgctgaccg taccggtggt
     5761 cgtcgtatcc accgaggagg ggttgaccag aatcccgcgc gccatccgtg aaggcagcct
     5821 ggccctgggc gctaccaaag ccgagacgct gttcaaggtc gtgctgccca tggcgagccc
     5881 gtcgatcctg accggcgtca tcctggcggt ggcacgagcc gccggcgaag ttgctccgtt
     5941 gatgctcgtc ggggtcgtga agctggcgcc cacgctgccg cttgacggga actttcccta
     6001 cctgcatctg gagcgaaagt tcatgcacct cggcttccac atctacgacg ttggtttcca
     6061 gagccccaac gtggaagccg cccgcccgct ggtctacgcg accgctctgc tgctggtgct
     6121 gatcatcatc attctgaacc tcgccgccat catgctgcgc agccgtctgg aagaccggta
     6181 ccggggggtg gaatgaccgt caaacgaaaa gaacgagcct cttctaagca gcgcgagcgc
     6241 gctgatcacc gcctccttta cggatgaacc cctagagatg accgacacga aacagaacgc
     6301 gtcaggatcg aacggcacga gcctgctggt ggaccgtctt tcccttttct acagcgggca
     6361 gaaagcattg cacgaaatct ccctcgagat tccgcgcaag caggcaacgg ctttcatcgg
     6421 cccctcgggt tgcggcaagt ccacgctgct gcgttgcttc aaccggatga acgatctgat
     6481 cgacggcgtg gaaaccaccg gcaggatcct gctcgacggc accgacatca attccaagga
     6541 agtcgatgtt gcccagctaa gaaggctggt cggcatggtc ttccagcgtc cgaacccgtt
     6601 tccgaaaacc atctacgaga acgtcgccta cggcctgcgg cttcaggaca cgctgccgaa
     6661 aaccgaaatg gacgagcgcg tcgaatgggc cctgcgaagc gccgcgttgt gggacgaggc
     6721 gaaggatcgt ttgaaagagt ctgcgctcag cctgtccggc gggcagcagc aacgactcgt
     6781 catagcacgc gccattgcca tcaagccgtc ggtgttgctc ctcgacgaac ctgcctccgc
     6841 cctggatccc atttcaacgc tcaaaattga ggaactgata catcaactga aaaccgagta
     6901 cactatagta atcgtaactc acaacatgca gcaggcagcc agggtatccg actacaccgc
     6961 gtttctttac ctgggggaac tgatcgaatt cggcaagacg gacactcttt tcacaaatcc
     7021 caaagagcgc cagaccgaag actacataac ggggcgctac ggctagacag aaacgcgaaa
     7081 ggacgcgaca ggaatacgaa atgacccccc atcctcacat atccgaacag ttcgatcacg
     7141 aactcgacca ggcacgcaac cgtctcatgg agatgggagg caaggtcgaa cagcagctga
     7201 agaacgcctg caaagccttg accaatcacg acctggaact cgccgagacg atcaagaccg
     7261 gcgatcgcag catcaaccag ctggaagtcg atctggacga gctctgcgtg cagataatcg
     7321 cccgccgcca acctgctgca tcagacctgc gcaccctcgt cagcgtgatg aaagccagca
     7381 ccgatctcga acgtattggc gacgaggcga aacggatcgc caagatggcc atcgcgatat
     7441 cacgtctgac ctatccgcgg gatcagtaca cggacatccg aaagctcgtc gcgttggtga
     7501 tggcccaggt ttcagcaacg ctggatgcgt ttgcccgact ggacgtcgac aaggcaatcg
     7561 gcttgatccg gtacgacgca gagatcgatg acgcctatga cgccatcgtc cgggaccgag
     7621 cggtacagat gaaggaaatg gccaataacc cggagggcat agaacagacc ctccacgttc
     7681 tctgggccgc cagggctctg gaacggatcg gcgatcatgc caagaacatc agcgaggtcg
     7741 tggtgttcct cgtcagaggc gaagatatcc gcgaccgcgc actggcggcc aagctgaccg
     7801 gcggctgagt taccttctca gcgacgcgcg aagggcatct gattcaaatg gtgcccgcca
     7861 tccagtatga gcgtctctcc cgtgaccacg tctgcaccgc tcagaaaata aacgatggcc
     7921 tcggcaatgg tgtccggcgt ggtggtcagg cgcagaggcg tggactcctc gaggaacttc
     7981 ttggtccttg cgtagatctc cgcgcccatg ccctgctcca gccaatcgcc ctgaatgaac
     8041 ccgggacaga ccgtgttcac gcgaatctcg gggccgagga ctctggcgag cgacaggccc
     8101 atggtaacga gcgctccctt ggaggccgcg taggcgatgg agcttccgat tccagtcacg
     8161 ccggcgatgg aggcgacgtt gacgatgctg cctctgcccc cccgtttcat ggccggcgcg
     8221 accgcccgcg tcatctggta ggggcccacc aggttcacgc cgtaaatgtt catgaagtcg
     8281 tccgaatcga ggccttcgag atcatgatga gcacagaact tggtcgtccc cgcattgttg
     8341 accaacgcgt ctatcctgcc ccaccgctct tcggccgtcg cagcaaggcg ccgacaatcg
     8401 gcgtcgtcgg ccacgttggc gcggacgacg gcagtttcca ccccgaacgc ttcgcaggct
     8461 tcctgggttg ctctggcgcc atcctcgttg ctcgcatagt tgatgaccac gtgggcgccc
     8521 cgctcggcca gctgacgagc tgtggccgca cccacacccg aagacgatcc ggtgacgatt
     8581 gccacacctt cctgaaggtc catccgcctg ctcccactct gcgcgaccgg gagaacctag
     8641 cagactcctc ctgcgcccac cagccgttgc ggtggaggct ccccggtcat tgatttagaa
     8701 tccgccgcct cctgagcaac ccatggacca aatgaccatc agcgcgattc tctgggactt
     8761 cggcggggtt ctgaccacaa gcccattcga ggccttcaat cggttcgaag cggagcacgg
     8821 gatccccgcc gacttcatcc gccgcatcaa tgccacgaac ccggatggca acgcatgggc
     8881 ccagttcgaa tccagcgcca tcgggctgaa cgagttcgac gaggcgtttg cccgggagtc
     8941 cgccgcgcag gggcaccgca ttcccggaag aaaggtcttg gagttgctct cgggcgagct
     9001 gcgtcccagg atggtcgaag ccctgcacag ctgcaaggtg tcgtatcagg tggcgtgcat
     9061 caccaacaac gtcaagtccg gccagggacc ggccatggca agcaaccggg cgcgagccgc
     9121 ggccatggaa ggggtcatgg cgctgttcga cctcgtggtg gagtccagca tcgaggggat
     9181 ccgtaagccc gacccgcgga tttatgagct cacgtgcgag cggctcggcg tccgacccga
     9241 gcaggccgtg ttcctcgacg acctcggcgt caacctcaaa cccgcgcgtg ccctgggcat
     9301 gcacaccatc aaagtggtcg aggaaagtca ggcgcttgaa gaactcggct ccctcactgg
     9361 gcttgcgctc tgagagggac gctcagccgc cctcgattcg ctgcatgaag aagacctcgc
     9421 tgtctggctc gaggggttcc agaaacgcgt cctgatagat ctggccatcg atggccaccg
     9481 cacacttctc cagcaccgcc cccgcctcgg gccaacgcct ttcgagattg atcaggagct
     9541 cgcgaaaatc ggccgcgggc acctcgatct cccgctctcc gcccgtgtgc tgaagcaggt
     9601 gctcgggaaa cacgactctg gccatgggtg ctggcgctcc ggggagaacc ggggacttcc
     9661 gtccccggtt gattcccgtc aatccagagc tgagagcacg cgcggcggcg acagcggcag
     9721 gtcgttcaca cgaacgcccg ttgccgctct gaacgcattc gccgtggccg ccagaggcgc
     9781 cacgatgggc gtctcgccca ccccccgcac gccaaacgga tggctcgggt tgggcacctc
     9841 gacgatgtga cagtcgatca tgggcagatc tgatgccacc ggaatccggt agtccaggaa
     9901 cccggcgttc tccatgaccc cgttctcgtc gaagatgtat tcctcattga gagcccagcc
     9961 gataccctgg gcagccccac cctgcatctg gccttcgacg tacgaaggat ggatggcctt
    10021 gcccgcatcc tggatggcgg tgaagcccaa gacgtcgact ttgcccgttt ctgggtccac
    10081 ctgcagatcg cagatgttca ccgagaacga tgcgccggcg ccctgggcat tgagcgatgc
    10141 gcgtcccagc agcggcccgc cggtcctgcc ggctttggcc gcgatgtccg cgaggctcaa
    10201 gggcgtgacg tcggcattca caccgggtgc cggcaccgcc tgaccgtctt tccaatccac
    10261 ctggtcggcg tccattcccc aggttgcggc cgctcgggct ttgcactggg tgatgatgtc
    10321 ctcgcaggcc tggaccacgg ccattcccgt cgcaaacgtc gtccggcttc cgcccgtcac
    10381 gtttgagaat ccggtgcttt cggtgtcgcc caccagggcc ctgatcttct cgtagggaac
    10441 accgagtgtt tcggacgcca tcatgcacat ggacgccctg gaaccgccga tgtcgggatt
    10501 gccctcgatg acggtcacgg tgccgtcctc gttgacgtgg acctcggcgc tcgactgcat
    10561 gccgacgttg aaccagaagc cggccgcaac acccctgcca gcaccctcag gcaacggctt
    10621 cgtgtagttc ggatgggctt ttgcggcctc caggcattct ttgaaaccga tctggggaaa
    10681 cttgggcccg tacggagcct gcgtcccctc gcccgccgcg ttcttcatcc tcagatcgat
    10741 gggatccatt ccgagctcga gcgccagatc gtcgagcgcg acttccatgg cgtgcatgga
    10801 ctgcggcgct cccggcgccc gataggcagc caccttgggt ttgttgagga gcacgtcaaa
    10861 ggcttctatt ttgaagttct ccaggtcgta gggcgcgaag atcgacatgg cacctgggcc
    10921 catgggagcc ccggcaaagg caccggcttc gtacgccagc caggctgttg ccgaggtgat
    10981 ggtcccgtcg cgcttggcac ccaccttgat ccggcaatgg gtgcccgagg tgggtccggt
    11041 ggcccgaaag acttcctccc gggtcatcat catcttcacc ggacgacggg acttcctggc
    11101 aagcagcaca gccagcggct cgaggtagac caccgtcttc ccgccaaagc cgccgccgat
    11161 ctccgaggca atgaacttga gatcggagat ttccatgttg agcattttgg ccgtcatcga
    11221 tcggaactcg aagtgaccct gagtggagca ccagatggtg gcctgccccc cttcgttgac
    11281 cgtcgccgtg caggcgtggg gctcgatgta gccctgatgg gccatgggaa tgttgtactc
    11341 gccctcgacg atgacatccg cctgagcgaa gccctcgtcc acgtcgcccc gggccagctg
    11401 catgacggca gcgatgttgc tcggtttgtc cggcgtctcg ggcaggtttt tggtgaagca
    11461 gtgatcgtcg agaagcggcg cgtcttcctt catggcctcc aggagatcga gaacgggcgt
    11521 gaggacctcg tactcgactt caatggcctc cagcgccgcg tcggccaccg acagcgactt
    11581 tgccgcgacc gccgccacgg cgtggccgtg ataaaggacc ttgtcgcggg ccatgacgtt
    11641 cttcgccaga tcggcgacgt cgcccccgcc ctcaccacca atctcaccgt gctttgcctc
    11701 cgggaagtcg gccccggaaa c
//
LOCUS       DBNHLM010000058         5259 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1084837, whole genome shotgun sequence.
ACCESSION   DBNHLM010000058 DBNHLM010000000
VERSION     DBNHLM010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1084837"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1745)
                     /locus_tag="AC16QF_02900"
     CDS             complement(183..1745)
                     /locus_tag="AC16QF_02900"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HIR6447530.1"
                     /translation="MAVQPLADITREHAKDRPDRRAMRYDEDGREWTYAELDADANRV
                     ANALLGDGVEPHGRIAYLDKNAPEYFLYLFGGAKINAVSVAVNWRLAAREMEYILNNS
                     EAGVLVIGEEFLGHLAQMDLPAVTRVVVLGDPGDTGYITFDQWIAGQPDDDPALPAAP
                     GDTCYQLYTSGTTGLPKGVELTHANFIHQVGSALAPLGIRSESVSLVCMPLFHIAGSG
                     FGVLGMYWGADMVLLRDVDLAAIVRVVPEQRVTHAMFVPAVLQFLLAQPGVRDADLSS
                     FETILYGASPITEAVLVEAMDVFGCDFLQGYGLTETTGGVSLLAAEDHDPGGPRAHLL
                     RSCGKVIVNHEVRIVDPESRREMPDGEVGEIWVRGPQVMKGYWKNPEASAESLVEDGW
                     FRTGDAGYLLDGYVFIHDRVKDMIISGGENIYPAEIENVLMAHEGIADAAVIGVPSER
                     WGETVKALVTRSDADLTEQQIIEYCRSQLAGYKCPTSVDWIDEIPRNPSGKILKVELR
                     KPYWEDRERFVS"
     gene            complement(1789..2574)
                     /locus_tag="AC16QF_02905"
     CDS             complement(1789..2574)
                     /locus_tag="AC16QF_02905"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6447531.1"
                     /translation="MTDGRLAGRTALVTGASRGIGRAIALAYGREGANVFLTATRLDN
                     LSDTRDRLADMGVEVACHAADVGKPDELETLFAAAAEWRDGVDVLVNNAGIYIGKPFE
                     AYSLEEFDRLMKINVYSVFQLMQLAIRHMTARGRGKIVNIASTAGKWESPNQAAYNTS
                     KHAVVGMTRCAALENASRGLNINAICPGLVETDMLGEFKTHADAQGISFDEFKRAVAA
                     QRIPMGRFLAPEEVAHIAVYLGSGESDGMTGQTITISGGMRMG"
     gene            complement(2597..4135)
                     /locus_tag="AC16QF_02910"
     CDS             complement(2597..4135)
                     /locus_tag="AC16QF_02910"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase family protein"
                     /protein_id="HIR6447532.1"
                     /translation="MRVLLLRALIASLCAGLAACQDPASGVGPTTGDLEPGRSDRPPN
                     VIILFADDLGYGDLGSYGHPTIRTPRLDALAAAGQRWTDFYAAAPVCSPSRGALLTGR
                     LPNRTGLYGRRLNVLFPNDPGGLPTAERTLAEALGERGYATAVVGKWHLGDRPEAYPT
                     RHGFDYWFGLPYSNDMDWVDAMSFEEVLALAAAGRMPEVEEEIARRQALYLDPRNEYW
                     NVPLVRSQRLATGITDEVVERPALQASLTSRYTDEAVAFIRRHQAQPFFLYLPYTMPH
                     TPLFPGDAFAGGSRGGRYGDVVEELDWSAGAIADALQELGLAENTLVVFTSDNGPWLA
                     MDHHGGSSGPLRDGKGTTFEGGVRVPAIFSWPGRIKPGVTVGIGSILDLYATALALAG
                     ADAGAAGDIDGLDLSGTLLEGRPSPRSSIAYYRSGELMAFRQGEYKLHLVTQGAYGRP
                     PERVVHEQPLLYHLGEDPGERFDAAGRHPDVVKRLQRAVAAHRASLVEQPPLFDRRLQ
                     PVHD"
     gene            complement(4139..4921)
                     /locus_tag="AC16QF_02915"
     CDS             complement(4139..4921)
                     /locus_tag="AC16QF_02915"
                     /inference="COORDINATES: protein motif:HMM:NF025668.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HIR6447533.1"
                     /translation="MDDLRYEQGGRLEVSVDVTNVGDRPGAETAQLYVAALTSGAYRP
                     FHELKGFAKTRLEPGETRRVTFVLDDGAFAVYDPEAKAWIVEAGEFELRVGASSRDIR
                     QTRIITVVSDQTLSPATEAVGGPNAGSPNAGSPNAGDPGSADAGLAVADAAFAAMLGR
                     PVPDPEPVRPYHLNSSVNEIAESWLGRLVKARVVAGFQQRMGARSSDETVQKMFEEMA
                     NNMPLRALPLFSRGAISFAQLEILVALLNGRVLRALRLWLSG"
     gene            complement(4934..>5259)
                     /locus_tag="AC16QF_02920"
     CDS             complement(4934..>5259)
                     /locus_tag="AC16QF_02920"
                     /inference="COORDINATES: protein motif:HMM:NF014026.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIR6447534.1"
                     /translation="VVVLSNGAPVEMPWVDRPAAVLEAYLGGQAGGSAAADLLFGLAN
                     PCGKLAETFPMRQADVPSDAWFPGSDRQVQYREGLYVGYRYFDTFDRGVLFPFGHGLS
                     YTRFE"
BASE COUNT          880 a         1846 c         1682 g          851 t
ORIGIN      
        1 cctacctcaa gctcaccttc gaagcttacc tcacggaaac ggtcgggtca cacccgccag
       61 aaaacagggg gttgacggag cgccccagca cgccacccag gtggaaacgc cgcccgcacg
      121 caccgaaacg gtgcgcgccc gcctcacgct ggggccgggc ccgaggaaac ctgggcgcgg
      181 gcttaagaaa cgaaacgttc ccggtcttcc cagtacggct tgcgaagctc gaccttgagg
      241 atcttgcccg acgggtttcg gggaatctcg tcgatccagt ccaccgaggt ggggcatttg
      301 taaccggcca gctgggaacg gcagtactcg atgatctgct gctcggtgag gtcggcatcg
      361 ctgcgcgtca ccagcgcttt gaccgtctct ccccaccgct cgctgggcac gccgatcacg
      421 gccgcatcgg caatcccctc gtgggccatc agcacgttct cgatctccgc agggtagatg
      481 ttctcaccgc ccgagatgat catgtccttc acgcggtcat gaatgaagac gtacccgtcg
      541 agcagatagc cggcatcccc cgtcctgaac caaccgtcct cgaccagact ctcggcgctc
      601 gcctcggggt tcttccagta gcccttcatc acctgggggc cgcgcaccca gatctcgccc
      661 acctcgccat cgggcatctc ccttctgctc tcgggatcga cgatgcgtac ctcgtgattg
      721 acgatgacct ttccgcagga gcgcagcaga tgcgccctcg ggccacccgg atcgtggtct
      781 tcagcagcaa gcaggctcac gccgccggtg gtttcggtca ggccgtagcc ctgcaagaag
      841 tcgcagccga acacgtccat ggcctccacc agcacggctt ccgtgatggg cgaagcgccg
      901 tacagaatgg tttcgaacga cgacagatca gcatcgcgta ccccgggttg ggcgagcagg
      961 aactgcagca ccgcggggac aaacatcgcg tgggtaacac gttgctcggg gaccacccga
     1021 acgatggcgg ccagatcgac gtcccgcagg agcaccatgt cggctcccca gtacatgccc
     1081 agcacgccga acccgctgcc ggcgatgtgg aacaagggca tgcagaccag gctgacggac
     1141 tcggatcgaa tgcccagggg cgcaagggcg ctgccgacct ggtgaatgaa gttggcatgg
     1201 gtcagctcca cgcccttggg cagccccgtg gttcccgagg tgtagagctg gtagcaggtg
     1261 tccccgggag cggcaggcag cgcggggtca tcatctggct gaccggcgat ccactgatcg
     1321 aacgtgatgt aaccggtatc gccggggtcg cccagcacca ccacccgggt caccgccggc
     1381 aggtccatct gggcgagatg gcccaggaac tcctcaccga tgaccagcac gcccgcttcg
     1441 ctgttgttga gtatgtactc catctcccgg gccgccaggc gccagttgac ggctacggag
     1501 acggcgttga tcttggcccc gccgaacagg tagagaaagt actcgggggc gttcttgtcc
     1561 aggtaggcga tgcggccgtg aggctccacg ccgtcaccga ggagcgcgtt cgccacgcgg
     1621 ttcgcgtcgg cgtcgagctc cgcgtaggtc cactcgcgcc cgtcctcgtc gtatcgcatc
     1681 gctctgcgat cgggcctgtc tttcgcgtgc tcccgcgtga tgtcggccaa cggctgaact
     1741 gccatgctcc ctcctcttga tccggttgat ccggctgaac gcgacggctc agcccatgcg
     1801 catgccgccg gatatggtga tggtctgccc cgtcatgccg tctgactccc cgctgccgag
     1861 atagacggca atgtgagcca cctcctccgg ggcgaggaag cgtcccatgg gaatccgctg
     1921 ggcggctacc gcgcgcttga actcatcgaa gctgatgccc tgggcgtccg cgtgggtttt
     1981 gaactcaccc agcatgtccg tctccaccag cccggggcag atggcgttga tgttgagccc
     2041 cctggaggcg ttctccagcg ccgcgcagcg cgtcattcct accaccgcgt gcttggaagt
     2101 gttgtaggcg gcctggttgg gactctccca cttgccggcc gtggacgcga tgttgacgat
     2161 cttgccgcgc cccctggccg tcatgtgccg gatggccagc tgcatgagct ggaacaccga
     2221 gtagacgttg atcttcatga gccggtcgaa ctcttccagg ctgtaggcct cgaagggctt
     2281 gccgatgtag atgcccgcgt tgttgacaag aacgtccacg ccgtcccgcc actcggcggc
     2341 ggcggcaaag agcgtttcca actcgtcggg cttgcccacg tcggcggcgt ggcaggcaac
     2401 ttccacgccc atgtccgcca ggcgatcccg ggtgtcagag agattgtcga gacgggtggc
     2461 ggtgagaaag acgttggcgc cttcccggcc ataagcgagc gcaatcgcgc ggccgatacc
     2521 acgactggcg ccggtgacga gcgccgttct gcctgcgagc cgaccgtcgg tcatctgatt
     2581 cctccccttg ctttgctcaa tcgtgcaccg gctgaagccg ccgatcaaac agcggcggct
     2641 gctcaaccag gcttgcccgg tgggcggcca cggcgcgctg aagtcgcttc accacgtccg
     2701 gatgtcgacc cgccgcatcg aaccgctcgc ccggatcctc ccccaggtgg taaagcaggg
     2761 gctgctcgtg gaccacgcgc tcgggcggcc ggccgtaggc gccctgggta accagatgca
     2821 gcttgtactc gccctggcgg aaggccatga gctcgccgct gcgatagtag gcgatactcg
     2881 agcgcgggct cggccggcct tccagcagcg tcccgctgag atccaggccg tcgatgtcgc
     2941 cggcggcgcc ggcgtcagca ccagccaggg ccagtgccgt cgcgtagaga tccaggatcg
     3001 agccgatacc gacggtcacc cccggcttta tgcggcccgg ccacgagaag atcgccggca
     3061 cccgcacacc gccctcgaag gtggtgccct tgccgtcccg cagcggaccc gaagacccgc
     3121 cgtgatgatc catggcaagc cacggcccgt tgtcgctggt gaacaccacc agggtgtttt
     3181 ctgcaagacc cagctcttgc agagcatctg cgatcgcgcc cgcgctccag tccagctcct
     3241 ccaccacgtc gccgtaacga ccgccccggc tcccgccggc aaaagcgtcg cccgggaaca
     3301 gaggcgtgtg gggcatcgtg tacggcaggt agaggaagaa cggctgcgcc tgatgacgcc
     3361 ggatgaaggc cacggcttcg tccgtgtagc ggcttgtcaa cgacgcctgc agcgccggcc
     3421 gctcgacgac ctcgtcggtg atgcccgtcg ccaatcgttg gctgcgcacc agcggcacgt
     3481 tccagtactc gttgcgtgga tcgagataga gcgcctgccg gcgcgcgatc tcctcttcga
     3541 cctcgggcat ccgacccgcc gcggccagcg cgagcacttc ctcgaaggac atggcgtcga
     3601 cccagtccat gtcgttggag tacggcagcc cgaaccagta atcgaacccg tggcgggtgg
     3661 ggtaagcctc gggccggtct ccgaggtgcc acttgccgac cacggccgtc gcgtagcccc
     3721 gctcacccag ggcttccgcg agggtgcgct ccgctgtcgg caacccgccg gggtcgttcg
     3781 gaaacagcac gttgagccgg cgcccgtaaa gccccgtccg gttgggcagc ctgccggtca
     3841 gcagcgcgcc ccggctgggc gagcagacgg gggcggccgc gtaaaaatcc gtccagcgct
     3901 gcccggcagc ggccagcgcg tccagccggg gcgtccgtat ggtcgggtga ccgtagctgc
     3961 cgagatctcc gtagccgagg tcgtcggcaa agagaatgat cacgttcggg ggccggtcgc
     4021 tccggccggg ctcgagatca ccggtcgtgg gccccacccc gctcgccgga tcctgacagg
     4081 ctgccaggcc cgcacacagc gacgcgatca gggcacgcaa gagcaggacg cgcacgcctc
     4141 agccgctcaa ccacagccgc agcgcgcgca gcacccgccc gttcagcagc gccaccagaa
     4201 tctccagctg cgcaaagctg atcgcccctc gcgagaacag cggcagggcc ctaagcggca
     4261 tgttgttggc catctcctcg aacatcttct gcacggtctc gtcgctgctg cgcgcgccca
     4321 tgcgctgctg gaagccggcc accacccgcg ccttcaccag acggcccaac cagctctcgg
     4381 caatctcgtt caccgacgag ttcaggtggt aggggcgcac cggctcggga tccggaaccg
     4441 gtcgcccgag catggcggca aacgccgcgt ccgccaccgc caggcctgcg tcggcgctgc
     4501 ccgggtcccc ggcgttcggg ctaccggcgt tcgggctacc ggcgttcggg cccccgacgg
     4561 cttcggtcgc cggcgacagg gtctgatcgg aaaccactgt gattatgcgg gtctggcgga
     4621 tgtcgcgact cgatgcgccc acgcgcagct cgaactcacc cgcttccacg atccaggctt
     4681 tggcctcggg atcgtagacg gcgaaggcgc catcatcgag aacgaacgtc acccgccggg
     4741 tctcgcccgg ctccagccgc gtcttggcaa accccttcag ctcatggaac ggccggtagg
     4801 cgcccgaggt cagagcggcg acgtagagct gcgcggtctc ggcgccgggg cggtcgccga
     4861 cgttcgtcac gtcgacgctc acctctagcc tgccgccctg ctcgtagcgc agatcgtcca
     4921 tggcgatctc gccctactcg aagcgggtgt agctgagccc gtggccgaac gggaagagca
     4981 ccccccggtc gaaggtgtcg aagtagcggt aaccgacgta tagaccctca cgatactgca
     5041 cctggcgatc agagcccgga aaccacgcat cggagggcac gtcggcctgg cgcatcggga
     5101 aggtctccgc cagcttgccg cagggattcg ccagcccgaa cagcaggtcg gccgcggcgg
     5161 aaccgcccgc ctgaccgccg agatacgctt cgagcaccgc cgccgggcga tcgacccagg
     5221 gcatctcaac gggcgcgccg ttgctcagca ccaccaccg
//
LOCUS       DBNHLM010000059         5775 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_486521, whole genome shotgun sequence.
ACCESSION   DBNHLM010000059 DBNHLM010000000
VERSION     DBNHLM010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_486521"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="AC16QF_02925"
     CDS             complement(<1..376)
                     /locus_tag="AC16QF_02925"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HIR6447535.1"
                     /translation="MGSRPPFIEQLRHAWRENASLLCVGLDPDPGRLPSGISTLEFCC
                     AIVDATAPFVCAFKPQAAYFAAQGREADLADLIAYIHDRHPGIPVILDAKRGDIGSTA
                     RLYAVEAYERFGADAVTVNPYLG"
     gene            667..1089
                     /locus_tag="AC16QF_02930"
     CDS             667..1089
                     /locus_tag="AC16QF_02930"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR6447536.1"
                     /translation="MKSNIIEFHAARAKAMSVEEQRLHARLRSDDRLFTQVVLSSQEP
                     EMVGATLSCTAVNLSIGGIQFRTNSPVPSGALLDLWVDISSRPGKFFLAGEVRWSRPT
                     GSINDRGEPEWFIGVQLKSGAATDIIDWRDFHANPTVR"
     gene            1272..3482
                     /locus_tag="AC16QF_02935"
     CDS             1272..3482
                     /locus_tag="AC16QF_02935"
                     /inference="COORDINATES: protein motif:HMM:NF019410.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 domain-containing protein"
                     /protein_id="HIR6447537.1"
                     /translation="MTPPTVVSLDSPERKLAIPPILQNVRSAAKRRLNDLLQELLNNA
                     DDALFEMANRSRNDTDENMFFEAMREIRLQRKKISKHFVDEFHRSFDDIFAAEEPTPR
                     GPIDESPDELSLLRNDELEVAVAVSGMVSKITSQFSLPIMQLTKRLDHACESQTITER
                     VNPLGPERISRAFVRAIEAINLDIKLRIIILKLFERFVMEQLGPTYEEANKLLAEAGV
                     LRDLSAMRGRASGGPGHGQSGAAATDHAGAHGDAGSSPVGGGGGSANFGIIQTLLSRF
                     TGDRSGVPIAATSGKTIETSQLLSMLSDVQGDRTVHSIDPASVPESLDLQAVLAARSA
                     QVLGHVNASPARDDGDAMNFVAMLFDYILNDRNLAIPMKALIARLQIPIIRLAIIDKS
                     FFEKSNHPARQLLNELSSAGIGWSAASELKRDALYNFIESIVLRVLNDFADNPDLFET
                     LVEELREFLKKDLKRRLLMEKRVKEIETGKAKTKAAKETVQNLINEMASGQRVPPEIG
                     KFISETWSKALIFACVTAGTESDLWNHHVRVMADLLKIIQPLQSEDDIAARDAVAPDL
                     IDTIEAALVDLKIADRERNDLITMLTEQLRQLSADDRAHVDEPESAAPVLAFETMRNV
                     TLTAPTEAEEQDPDDPPPPQYVDEVNRLEEGAWLEFVDEHGDNVRVKLAGIIEPGARY
                     VFTNRRGIRVFKKSKMQLANDLQNQTVMVLDESQVFDRALQAVISKLRTEEAEE"
     gene            complement(3532..4176)
                     /locus_tag="AC16QF_02940"
     CDS             complement(3532..4176)
                     /locus_tag="AC16QF_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase subunit alpha"
                     /protein_id="HIR6447538.1"
                     /translation="MFTGIVQGLCPVTGVDDKPGLRCIAIKLGKLAGGIQTGASVAIN
                     GTCLTVTSAAKGVVSFDVIRETLENTNLGDLQRGSLVNVERSFRAGDEIGGHILSGHV
                     IGKVRVAAVEEDENERNVRFDVRAEWIKYLPHKGFVALDGASLTIARVDATNDQITVC
                     LIPETIARTTLGSAEPGSWVNLEIDAQTQAVVDTVERVLSSPEWQRRLRGEPSS"
     gene            complement(4248..5585)
                     /locus_tag="AC16QF_02945"
     CDS             complement(4248..5585)
                     /locus_tag="AC16QF_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6447539.1"
                     /translation="MKFCSHIVRLALSVTLCFAAIPFAGEFGLSGSQAFAEEGEGQQQ
                     KTRRVPSMSEATFKKLAEAQELIDAKDYQGAVTSLNEMLTSRRRSLNGNEVGQVHNML
                     GFVYFSMEDYPKAIEMYKVVVDQEEDIPEGLEVTTLYTLAQLSFVAERYRDALKYMEI
                     WLSKAQNPGPEPHIFMGQVYYQMQDFRRSIVQIEKGIGVAQERGTPVKENWWSLLNYL
                     YFEEENWPKVIEILEILVRDFPKREYWIRLAGIHGQEGNEREQIGSMEAAHVAGFLTR
                     ERDLTNYAGLLMQAEVPIRAAKVLDAGVEAGVIEKTATNLRSLGQAWQLAQETKKAIP
                     VFEEAGKLADDGAIYERLAQLYLDNDEFSKCVRAAGRAIDKGGLRKVQTTYVVRGMCE
                     YNQDRLPQARSSFVSCRNESRRAKDETNRRICQQWITYLDRETTRREQLEKAI"
     gene            complement(5616..5765)
                     /locus_tag="AC16QF_02950"
     CDS             complement(5616..5765)
                     /locus_tag="AC16QF_02950"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6447540.1"
                     /translation="MIIESKPPGIFDRAALQAALKFKYKPKVVNGEPIDVAGVRNRIT
                     FELQD"
BASE COUNT         1187 a         1820 c         1672 g         1096 t
ORIGIN      
        1 tgccgagata gggattgacc gtgactgcat cagcgccgaa acgctcgtac gcctctacgg
       61 catacagcct cgccgttgag ccgatatcgc cgcgtttggc gtccagaatg acggggatac
      121 ccgggtggcg atcgtgaatg taggcgatca ggtcagcgag gtccgcctcc ctgccctggg
      181 cggcgaagta ggctgcctgg ggcttgaacg cacacacgaa gggcgccgtg gcatcgacaa
      241 tcgcgcagca gaactccagg gtggagatcc cggaaggcaa ccgcccggga tcagggtcca
      301 gaccaacgca aagcagggaa gcgttctcgc gccatgcgtg gcgcagctgc tcgatgaaag
      361 gcggacggga acccacggcc ccgagtatat gcgtaactga tcttccgcgt cacaaggatg
      421 accgccaggg tcatttgtaa agacgcctta tgacctaagg cgaccactaa aaacgttcca
      481 tatctgtttg tttttattat attatcgcga tgtcactggg gggtggcacg ccgatggcac
      541 gattctcgca acgctacgat cggtaacgat cggtgcaggg aactcgtgat ttgcacgtcg
      601 acgataagag ttcttacgag ccgaacgcac aaagccaata aaggtttagg cgggtagaga
      661 acgaaaatga agtcgaacat catcgaattt cacgcggcgc gcgccaaggc aatgagcgtc
      721 gaagagcagc ggctccacgc caggcttcgc agcgatgacc ggctgttcac tcaggtcgtt
      781 ctgtcctccc aggagcccga gatggtcggc gccacgcttt cctgcaccgc agtaaacctc
      841 tccattggcg ggatccagtt ccgcaccaac tcccccgtgc cttcgggcgc gctcctggac
      901 ctctgggtgg atatctcgag ccggccgggc aaattttttc tggccggtga agtccgctgg
      961 agccggccca ccggatccat caacgaccgg ggcgagcccg aatggttcat cggcgtgcag
     1021 ctcaagtcgg gagcggccac cgacatcatc gactggcggg actttcacgc caaccccacg
     1081 gtccgctgaa ggccgaacgg cccttgcgtc ctggaatcga agctctgaga gcttcacaga
     1141 ctcgacggtt cccaacgaac actcaattcg tgaacccgtt caccttttcg ccgtaatccg
     1201 catcccgtcc ccggaccaac gaatctgctg taatcgttct accttaaggt ctttgtggga
     1261 gcaggcgctt cgtgacccca ccaacagtgg tttcactcga ctcgccggaa aggaaactgg
     1321 ccattccgcc gattctgcag aacgttcgga gcgcggcaaa acgccggctc aacgatctgc
     1381 tgcaggaact cttaaacaac gccgacgatg cgctgttcga gatggccaat cgctcccgca
     1441 acgacaccga cgagaacatg ttcttcgagg cgatgcgcga gatccgcctg cagagaaaaa
     1501 aaatctccaa gcacttcgtg gacgagttcc accgctcctt cgacgacata ttcgctgcag
     1561 aggagcccac accccgcggg ccgatcgacg aaagcccgga cgagttatcg ctgttgcgca
     1621 acgacgagct tgaggtggcc gtggcggtga gcgggatggt ctccaagatc accagccagt
     1681 tttccttgcc catcatgcag ctcaccaagc gcctggacca cgcctgcgag agccagacga
     1741 tcaccgagcg cgtaaatccc ctcggtccgg agcggatcag ccgcgcgttc gtgcgggcca
     1801 tcgaagccat aaacctcgac atcaagcttc gcatcatcat cctgaagctc ttcgagcgct
     1861 tcgtcatgga gcagctcggg ccgacttacg aagaagccaa caagctgctg gccgaggccg
     1921 gcgtgctgcg tgatctgagc gccatgcggg gccgcgcgag cggcggcccg gggcatggac
     1981 aatcgggtgc ggccgcaacc gatcatgccg gggctcacgg tgatgccggc agctcccccg
     2041 ttggcggcgg aggaggttcc gccaatttcg ggatcatcca gacactgctt tcaagattca
     2101 ccggtgaccg atctggcgtt cccattgcgg ccaccagcgg caagaccatc gagacgtccc
     2161 aactcctctc gatgctctcc gacgtccagg gagaccgcac ggttcattcc attgatcccg
     2221 ccagcgtgcc ggaatcgctg gatctgcagg ccgtcctcgc ggccaggtcg gcgcaggtgc
     2281 tcggtcacgt aaacgcaagc cctgctcggg acgacggcga cgcgatgaac ttcgtggcca
     2341 tgctgttcga ctacatcctc aacgatcgga acctcgccat tccgatgaag gccctcatcg
     2401 cccggctgca gattccgatc atacgcctgg cgatcatcga caagtcgttc ttcgagaagt
     2461 ccaatcatcc tgcccgccag ctcctcaacg agctctccag cgcgggcatc ggctggagcg
     2521 cggcctccga gctcaagcgg gacgcgttgt acaacttcat cgaatcaatc gtgctgcggg
     2581 tcctgaacga ctttgccgac aacccggatc tgtttgaaac tctggtcgag gaacttcggg
     2641 agttcctcaa gaaggatctc aaacggcgtt tgctcatgga gaagcgcgtc aaggagatcg
     2701 agaccggcaa ggccaagacg aaagccgcca aagagaccgt tcagaacctg atcaacgaga
     2761 tggccagcgg gcagcgcgtg ccccctgaga tcggcaagtt catcagcgag acctggagca
     2821 aggcgctgat cttcgcctgc gtgacggcag gtaccgaatc agacctttgg aaccaccacg
     2881 tgcgcgtgat ggccgacctg ctcaagatca tccagccctt gcagagcgaa gatgacattg
     2941 cggccagaga cgcggtggcc cctgatctca tcgacaccat cgaggcggct ctggtagatc
     3001 tgaagatcgc cgatcgcgaa cgcaacgacc tgatcaccat gctcaccgag caattgcgcc
     3061 agctgtcggc ggacgatcgg gcccatgtcg acgagccgga aagcgcggcg cccgttctcg
     3121 ctttcgagac catgcgcaac gtgacgctga ccgctcccac cgaagcagag gagcaggatc
     3181 ccgatgatcc gccgcccccg cagtacgtgg acgaggtcaa tcgtctggaa gaaggagcct
     3241 ggctcgagtt cgtcgacgaa cacggcgaca acgttcgggt caagctagcc ggcatcatcg
     3301 agccgggagc gcgctacgtg ttcaccaacc gccgggggat acgggtcttc aagaagagca
     3361 agatgcagct tgccaacgac ctgcagaacc agaccgtgat ggttctcgac gagtcccagg
     3421 tgttcgaccg cgccctgcag gcggtcattt ccaagctgag aaccgaggaa gccgaagaat
     3481 agatcgggcg caccccgatg ccgattggcc tcgaagggct gagagaaacg gtcaggacga
     3541 cggttcgccc cgcagacgcc tttgccactc cggcgagctc agcaccctct caaccgtgtc
     3601 caccacggcc tgggtctggg cgtcgatctc cagattgacc cacgatcccg gctcagcact
     3661 tcccagcgtc gtgcgggcaa tggtctcggg gatcagacat accgtgatct ggtcattggt
     3721 tgcatcgacc ctggcaatcg tcaggctcgc tccgtccagc gccacgaagc ctttgtgggg
     3781 cagatacttg atccattcag cacgcacgtc gaagcgcacg ttccgctcgt tctcatcctc
     3841 ttcgacggcc gcgacgcgaa ccttgccgat gacgtgaccc gacaggatgt gaccccctat
     3901 ctcgtccccc gcacggaatg accgttcgac gttgaccaag gagccgcgtt gcaggtcgcc
     3961 gaggttcgtg ttctcaagcg tctcgcgaat gacgtcgaaa gacaccacgc cttttgcagc
     4021 agacgtcacg gtcagacagg ttccgttgat ggcgacggac gcaccggtct ggataccacc
     4081 cgccagcttg cccagcttga tggcgatgca tcgaaggccc ggtttgtcgt ctacgccagt
     4141 gaccgggcag aggccttgga cgataccggt gaacattgga gccctttacg cagagggccg
     4201 gcaatgccgg cccggacgtg ggacgtcagt cgggagcagg ttcggcttca gatggccttt
     4261 tccaactgct cgcgccgcgt ggtttcgcga tccagatagg tgatccattg ctggcagatt
     4321 cgccggttgg tctcgtcttt ggcgcgccgc gactcgttac gacaggagac gaacgatgac
     4381 cttgcctggg gaagccggtc ctgattgtac tcacacatgc ctcgcaccac gtaggtggtc
     4441 tgaactttgc gcagcccacc cttatcgatg gcccttccgg ccgcacgaac gcacttgctg
     4501 aactcgtcgt tgtcgagata gagctgggcc aaccgttcgt agatggcccc atcgtccgcg
     4561 agtttgcccg cctcctcgaa aacgggaatc gccttcttgg tttcctgggc cagctgccat
     4621 gcctgcccaa ggctccgaag attcgtggcc gtcttctcga tcacgccggc ctcgacgccg
     4681 gcgtcgagga ccttggccgc tcgtataggc acctcggcct gcatcagcag cccggcgtaa
     4741 ttggtcagat cccgctcgcg ggtgaggaag cccgccacgt gcgcggcctc catggatccg
     4801 atctgctccc gctcgttgcc ctcctggcca tggatgccgg caaggcggat ccagtactcg
     4861 cgcttaggga agtcgcgaac gagaatctcg agtatctcga tcactttggg ccagttctcc
     4921 tcctcgaaat acagatagtt gagcagcgac caccagttct ccttgacggg cgttcccctt
     4981 tcctgggcga cgcctatccc cttctctatc tggacgatcg agcggcgaaa gtcctgcatc
     5041 tggtaataga cctggcccat gaaaatgtgg ggctcgggcc ccgggttctg agctttggag
     5101 agccagattt ccatgtactt gagcgcgtcc cgatagcgct cagcgacgaa gctcagctgc
     5161 gccagggtgt atagcgtcgt aacctcgagg ccctcgggaa tatcttcctc ttgatcgacg
     5221 acgaccttgt acatctcaat cgccttgggg taatcctcca tggagaagta gacgaaaccg
     5281 agcatgttgt gcacctgccc gacctcgttg ccgttaagcg accgacggcg actcgtcaac
     5341 atttcgttca gggacgtgac cgcgccctga taatctttgg cgtcaatcag ctcctgcgcc
     5401 tctgcaagct ttttgaaagt cgcctcgctc atggaaggca ctcgacgcgt cttctgttgc
     5461 tggccctcgc cttcctcggc gaaggcctgg gaaccgctca ggccgaattc acccgcaaac
     5521 ggtattgccg cgaagcacaa cgtcacggac agcgccaggc gaacgatgtg agaacaaaac
     5581 ttcaaatcta cctcccggct tagccccaag ccccgtcaat cctgaagttc gaacgtaata
     5641 cggttacgca caccagcaac gtcgatgggc tcgccattga ctactttggg cttgtacttg
     5701 aatttcaaag cggcctggag cgcagcccga tcgaagattc caggcggctt ggattcgata
     5761 atcacgggat cccgg
//
LOCUS       DBNHLM010000060         8250 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_128173, whole genome shotgun sequence.
ACCESSION   DBNHLM010000060 DBNHLM010000000
VERSION     DBNHLM010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8250
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_128173"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1581
                     /locus_tag="AC16QF_02955"
     CDS             262..1581
                     /locus_tag="AC16QF_02955"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6447541.1"
                     /translation="MTVYRKIERFADPQTSRTPPQKEPDPDLGYDIIPKERYTSPEFM
                     QLEWDRMWTKVWLMGAWIGDLQNPGDYVVTEIGKESIVITRDENGDYNAFYNVCSHRG
                     NQVAYGKGGNTKTFRCSYHLWEYNLRGEIVNVPDQETFPQGVPCEKLAIVKLPCAAWC
                     AWVWFSLNPDVEPLEDFLGMIPEHLDPYHFEKMALVNDVTVEWDVNWKASVDAFNETY
                     HVAGTHPQLMWMLEDMDVQIDCYEKHNRYLIPFGCVSTHIEDGTPIAEALRVFMESYG
                     MDPDNFDGDGLEVRRGIQKWVRANAEVQGFDYSELNDDQLTDDYHYMIFPNITFNTHC
                     NSVMVFRQRPHPTDPNKMFYDVQNYSMMPAGTEWPERPPHRQWKHGEDTLGEVLDQDA
                     SNLPMVQRGMNSTGFRGLWISSQELRIRHFHKTLDDYLHRQPIKWMT"
     gene            1583..1927
                     /locus_tag="AC16QF_02960"
     CDS             1583..1927
                     /locus_tag="AC16QF_02960"
                     /inference="COORDINATES: protein motif:HMM:NF025178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR6447542.1"
                     /translation="MTPVAPQNALDDGEMMACNVNGVDVLLCRVDGQYYAVSNRCSHA
                     RQVLSTGKLRGHEINCPLHGARFDIRNGHCLAAPASQPIKTFPVTLEGGKVHVTVTAD
                     DLPARPRFGPLN"
     gene            1973..2731
                     /locus_tag="AC16QF_02965"
     CDS             1973..2731
                     /locus_tag="AC16QF_02965"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF048554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6447543.1"
                     /translation="MDLQLKGSKALITGGTKGIGRAIANALADEGCNVAICARNAEEV
                     EAAVADLSAKGVTAFGQAVDVGDGDAFKAWIAASAEALGGIDCIVSNVSGGGAQGEDA
                     WRRHLEYDMLGAVRMVEGAMPYLEKSENASVVLISTTAALEKFMSAGPYNALKAALLN
                     YNGALSQDLAPKGIRVNAVSPGPIFIEGGAWDNIKQHMTAFYDATVADIPIGRMGTAE
                     EVAAQVAFLASPRAGFTTGTNIVIDGGLTKRIQF"
     gene            2858..3604
                     /locus_tag="AC16QF_02970"
     CDS             2858..3604
                     /locus_tag="AC16QF_02970"
                     /inference="COORDINATES: protein motif:HMM:NF018038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR6447544.1"
                     /translation="MAERIIDVYMVANGLYHDIDYARSELLKLLGEHENVRVKVAANY
                     SDIEGIAASDFLITYCCHLIPSEEEQVALRDYVNSGKRWIALHGTNAILEFVEGGVDC
                     PETAPIFMETLGSQFMAHPPIQPFPVKVSDPEHELVAGIGEFETDDELYICKYHGEHH
                     ALLHTNFTGSCDGFINRDWPDDEPRVMYYLRKLGDGEVLYNCLGHCRGHYDMRPVTDY
                     YPAIERGSWDKPEYYELLRRSIKYCIDRVV"
     gene            3631..4596
                     /locus_tag="AC16QF_02975"
     CDS             3631..4596
                     /locus_tag="AC16QF_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6447545.1"
                     /translation="MAKPFRFGFQTFNAESGQSWADKARKAEDLGYSSFHLADHLLGP
                     GPALTAANHPIQNVAAIPAMAFAAAVTSTINIGCRVFCIDYHLPVVLIKSAMTIDMLS
                     NGRLELGLGAGWVKSEYEAVGIDFDEPRVRIDRLADVIAGVKAFRGEDLVDVHNDTLS
                     WKDFEGLPKPVGRAPIMVGGGSPRVLRLAGREADIVSLNFNNRMGMIGPDGVQMSSEQ
                     ETRKKIGWIREGAGDRFDDLEIEIGAYFTVVTDDAKPVVAGMAQMFGMSEDEMLRHPH
                     GLFGSVDAICDELQRRREEFGISYFTVGEDNLEAFAPVVARLSGK"
     gene            4593..5954
                     /locus_tag="AC16QF_02980"
     CDS             4593..5954
                     /locus_tag="AC16QF_02980"
                     /EC_number="5.4.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR6447546.1"
                     /translation="MTTVAEARAELAARTAGSQEISEARAEILNSGLAANLEMPHPIF
                     IESGEGPFVFDADGNRYIDTSVGFGLHILGHRHPLIEQAIKDRTEKGWMFGIHSTSQM
                     KLAELLHEASPCAERVVFCNTGSEATMYAIRAARGYSGKEKIALFDGFYHGAHDYGMW
                     LTDPTSPREAPRKLPMGRGIPRALADLTLLLPYRHEAAFDLIRRHADELALVMVEGVQ
                     SSNPQPGAGEFLCELEAVCREAGVLFAVDEVITGFRVAYGGAQERFGVRPDLATYGKI
                     LGGGLPVGAITGRADVMEVFTGLGASNGVFSGGTFSGNPLTMEAGAAAVGYLRDHPDV
                     YRGLEQAGERLAGAVNGFCQERQIPAQMKSVGSMFHMFFQREPIRSMRDVTGASVAAE
                     KAFYLHALNRGVLVPGTQRAFLSAAHSDAEIDTLIDVFQTSLADVQAEGLFSARTDKG
                     EMR"
     gene            5951..6541
                     /locus_tag="AC16QF_02985"
     CDS             5951..6541
                     /locus_tag="AC16QF_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6447547.1"
                     /translation="MSRLQKIEPEDWAPELKAAINPENLTPLEQGLTRFFAHRPELTL
                     GLMQFGGALKTKRTLPDKLIELVRLRVAFFNQCRSCMAIRYTDALNDGVTEDLVCSLE
                     KPAEAGNLTDAEKAAIRFGELMATDHLAIDDAVYDELRKHFSESEIVELGMHVAFFVG
                     FGRLAATWHMVEELPAAFQDDSRALSPWNEESIVVR"
     gene            6689..7891
                     /locus_tag="AC16QF_02990"
     CDS             6689..7891
                     /locus_tag="AC16QF_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6447548.1"
                     /translation="MDFEFSKEDQAFREEVRAFLEEKVTPDMRQAVRKTTTVFADKEL
                     AMRWQAILVEKGWAVPAWPAEHGGTGWSVTQKYIWGEECARASAPGLIPLGLRMLAPV
                     IFKYGTEEQKNYYLPRMLSGEHYWCQGYSEPGSGSDLSSLKTSAVKDGDDYIVNGTKI
                     WTTHAQFADHIFCLVRTDTGVRPQAGITFLLIPMDTPGIKVEPIITMAGDHEVNQVFF
                     DDVRVPQANRVGPENDGWNVAKYLLEFERGGGGAAVRLKESLKELRNIASSELADGEP
                     LMDDPGFRNRISELEIRTSALEFAELASLARMSRGESPGTASSLAKNTSVEIDQGILT
                     LKLEAVQHYAMPHANKISLDGHNEPWPGPDHAETVTPRYLNVRAASVYGGSKEVQKNI
                     IAKAVLGL"
     gene            7976..>8250
                     /locus_tag="AC16QF_02995"
     CDS             7976..>8250
                     /locus_tag="AC16QF_02995"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914905.1"
                     /note="catalyzes the formation of malate from glyoxylate
                     and acetyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HIR6447549.1"
                     /translation="MSATKRVSRGKLSVAAELDDLVSQRIAPGTGVDAEAFWAGFESI
                     VDDLAPRNRALLARRDELQATIDEWHEDRRGRPLDAQEYRAFLEQIG"
BASE COUNT         1589 a         2588 c         2681 g         1392 t
ORIGIN      
        1 ggcaacagcc gcatcgtcta cctgcagtgc ggagacgacc cggtggccta cgccaacccc
       61 cattttcaga agctgatccg caacgcgatc ttctgggttt cggaggcgtc ggctcggggc
      121 cgcgccggga gcggcggccg cacgggtgcc tgaacgggca tgcgggggtg caaaccggcg
      181 gcggtggtgg tacaaatcgc ctgttaaaac ctgcacagtt gactgttttc agctggcagc
      241 caagagagga ggattaacgg catgacggtt tatcgcaaga tcgagcggtt cgcagacccg
      301 cagacttcga ggacgccccc tcagaaggaa cctgatcctg atctgggcta cgacattatt
      361 ccgaaagagc ggtacacgtc ccccgagttc atgcagctcg agtgggatcg gatgtggacc
      421 aaggtctggc tcatgggtgc ctggatcggc gatctgcaga atccgggcga ctacgtcgtc
      481 accgagatag gcaaggagag catcgtcatc acccgggacg agaacggcga ttacaacgcg
      541 ttctacaacg tctgctctca tcgcggtaac caggtggcgt acggtaaggg cggcaacacc
      601 aagacgttca ggtgctccta ccacctctgg gagtacaacc tgcggggcga gatcgtcaac
      661 gttcccgacc aggagacctt ccctcagggc gttccctgcg agaagctcgc catcgtcaag
      721 ctcccctgcg ccgcgtggtg cgcctgggta tggttcagcc tcaacccgga cgtggagccg
      781 ctggaagatt ttctcggcat gatccccgag cacctggatc cctaccactt cgagaagatg
      841 gcgctggtca atgacgtcac ggtggagtgg gacgtcaact ggaaagcttc ggtggatgcg
      901 ttcaacgaaa cctatcacgt ggccggaacc catccgcagc tcatgtggat gctcgaagac
      961 atggacgtgc agatcgactg ttacgagaag cacaaccggt acctgatccc cttcggctgc
     1021 gtgagcacgc acatcgagga cggcacgccg atcgcggagg cgctccgggt cttcatggaa
     1081 agctacggaa tggatccgga caacttcgac ggcgacggtc tcgaggtgcg gcgcggcatc
     1141 cagaaatggg tgcgtgcgaa tgccgaggtc cagggcttcg attactcgga actcaacgac
     1201 gatcagctga ccgacgacta tcactacatg atcttcccga acatcacgtt caacacccat
     1261 tgcaacagcg tgatggtgtt caggcagcgt ccgcatccca cggacccgaa caagatgttc
     1321 tacgacgtgc agaactactc gatgatgccg gccggcacgg agtggcccga gcggccgcca
     1381 catcgtcagt ggaagcacgg cgaggacacc ctgggagagg tgctcgatca ggacgccagc
     1441 aacctgccca tggttcagcg cggcatgaac agcacgggat tccgcggtct gtggatctcc
     1501 agccaggagc ttcgcattcg tcatttccac aagacgctcg acgactatct ccacagacag
     1561 ccgatcaagt ggatgaccta aaatgacgcc ggtggcccct cagaacgccc tcgatgatgg
     1621 cgagatgatg gcttgcaacg tcaacggcgt tgacgtgcta ttgtgccggg tggacgggca
     1681 gtattacgct gtgtccaacc gctgcagtca cgccaggcag gtgctctcga cgggcaagct
     1741 gcgcgggcac gagatcaact gcccgctgca tggtgcccgt ttcgacatcc gcaacgggca
     1801 ctgccttgcg gcgccggctt cgcagccgat caagacattt ccggtgactt tggaaggggg
     1861 caaagttcac gtaacggtta ccgctgacga tctgccggcc cgtccccgat tcgggccgct
     1921 caactaacgc agctccagac agctgggaaa cgaaaaagag aggacactga cgatggattt
     1981 acaactcaaa ggatcaaaag cgctgatcac cggtgggacc aagggtattg gccgggcgat
     2041 cgcgaacgcc ctggccgacg agggctgcaa cgtggccatc tgcgcacgca acgccgaaga
     2101 ggtcgaggct gccgtggccg acctgagcgc caagggcgtc accgctttcg gccaggccgt
     2161 cgacgtgggt gacggagacg cgttcaaggc ctggatcgcc gccagcgccg aagcgctcgg
     2221 cggcatcgac tgcatcgtct ccaacgtcag tggcggtggt gcgcagggag aggatgcctg
     2281 gcgcaggcac ctcgagtacg acatgctcgg cgcggtccgg atggtcgagg gcgccatgcc
     2341 ctatctggaa aaatccgaga acgccagcgt ggttctcatc tcgaccactg cggccctcga
     2401 gaaattcatg agcgcgggtc cttacaacgc tctgaaagcg gcgcttctga actacaacgg
     2461 tgctctgtcg caggatctgg cgcccaaggg cattcgcgtc aacgccgtga gccccggccc
     2521 gatcttcatc gagggcggtg cgtgggacaa catcaagcag cacatgaccg cgttttacga
     2581 tgccaccgtc gccgacatcc ccattggacg catgggcacg gcggaggaag tcgcggcgca
     2641 ggtcgcgttt cttgccagcc cgcgggcggg attcaccacc ggtaccaaca tcgtcataga
     2701 cggcggcctg acgaaacgca tccagttctg aggtgcgcgc ggtcgtctga agggataccg
     2761 cgggccggcg ttgccgaccc gcggcgcctt tcgccgtctg atatctgata ggtagttaaa
     2821 ggacgctaca gcaacctgat ctctggggga gaacaccatg gcagagcgca tcatcgacgt
     2881 gtacatggtc gccaacggtc tgtaccacga catcgactac gcccgctcgg agctgctcaa
     2941 gctcctcggg gagcatgaga acgtccgggt gaaggtcgcg gccaactact cggacatcga
     3001 aggcatcgcc gccagcgatt ttctgatcac ctactgctgc cacctgattc ccagcgaaga
     3061 agagcaggtg gccctgcggg actacgtcaa ctcgggcaag cgctggatcg cgctgcacgg
     3121 caccaacgcg atcctcgagt tcgtcgaggg aggcgtggac tgtccggaga ccgcgcccat
     3181 cttcatggag accctgggct cgcagttcat ggcgcacccg cccatccagc ccttccccgt
     3241 gaaagtcagc gaccccgagc acgagctcgt ggccgggatc ggcgagttcg aaaccgacga
     3301 cgagctgtac atctgcaagt atcacggcga gcaccacgcg ctgctgcaca ccaacttcac
     3361 cggatcctgc gacggcttca tcaaccggga ctggccggac gacgagccgc gggtgatgta
     3421 ctacctgcgc aagctgggcg acggcgaagt gctgtacaac tgtctcggac actgccgggg
     3481 ccactacgac atgcgcccgg tcaccgacta ttaccccgcc atcgagcgcg gcagctggga
     3541 caagcccgag tattacgagc tgctgcggcg gtccatcaag tactgcatcg accgcgtggt
     3601 ctagcgggca gctgccaggg agagccgagc atggcaaagc cgtttcgttt cgggttccag
     3661 acgttcaacg cggagtcggg ccagagctgg gcagacaagg ctcgcaaggc cgaggatctc
     3721 ggctactcgt cgttccatct ggccgatcat cttctggggc cggggccggc gctgaccgca
     3781 gccaaccacc cgattcagaa cgtcgccgcc atccccgcca tggcgtttgc agcggcggta
     3841 acgagcacca tcaacatcgg ttgccgggtg ttctgcatcg actatcacct gcccgtggtg
     3901 ctgatcaagt ccgccatgac catcgacatg ctgtccaacg gccggctcga gctgggtctg
     3961 ggcgccggct gggtgaagag cgagtacgaa gcggtgggta tcgacttcga cgagccccgc
     4021 gtgcgcatcg accggcttgc cgacgtcatt gccggggtga aggcgttccg cggcgaggac
     4081 ctggtcgatg tccacaacga cacgctgtcc tggaaggact tcgaaggtct gcccaagccc
     4141 gtgggacgcg ctcccatcat ggtcggcggc ggcagccccc gggtgctgcg cctggccgga
     4201 cgggaggccg acatcgtcag cctgaacttc aacaaccgca tgggcatgat cgggcccgac
     4261 ggcgtgcaga tgtccagcga gcaggagacc cgcaagaaga tcggctggat ccgcgaaggc
     4321 gccggcgatc gcttcgacga tctggagatc gagatcggcg cctacttcac cgtggtgacc
     4381 gacgacgcca agcccgtggt cgcgggcatg gcgcagatgt tcggcatgtc cgaggacgaa
     4441 atgctccgcc acccccacgg tctcttcggc agcgttgatg ccatctgcga cgagctgcag
     4501 cgtcgccgcg aggagttcgg catctcctac ttcaccgtcg gagaggacaa tctggaggcc
     4561 tttgcgccgg tggtcgcgcg cctttccggc aaatgaccac ggtcgctgaa gcgcgcgccg
     4621 agctggccgc cagaacggcc ggatcccagg agatctccga ggcccgcgcc gagattctca
     4681 actcggggct cgctgccaac ctggagatgc cccatccgat cttcatcgag agcggcgagg
     4741 ggccgttcgt cttcgatgcg gacggcaacc gctacatcga cacctccgtg ggcttcggcc
     4801 tgcacatcct cggccatcgg cacccgctga tcgagcaggc catcaaggac cgcacggaga
     4861 aagggtggat gttcggcatt cactccacca gccagatgaa gctggccgag ctcctgcacg
     4921 aggccagccc ctgcgccgag cgggtggttt tctgcaacac gggttccgag gcgaccatgt
     4981 acgcgatccg tgctgcccgt gggtactctg gcaaggagaa gatcgctctg ttcgacggct
     5041 tctatcacgg tgcccacgac tacgggatgt ggctgaccga cccgacaagc ccccgggaag
     5101 cgccgcgcaa gctgccaatg gggcgcggca tcccgcgggc gctggcagac ctgacgctgc
     5161 tgctgccgta tcgtcacgag gccgccttcg atctgatccg ccgtcacgcg gacgagcttg
     5221 cgctggtcat ggtagagggt gttcagagct cgaatcccca gcccggggcc ggcgagtttc
     5281 tatgcgagct cgaagccgtc tgccgtgaag cgggcgttct gtttgccgtt gacgaggtca
     5341 tcacgggttt ccgcgtcgcc tacggcggtg ctcaggaaag attcggcgtg aggccggacc
     5401 tcgccaccta cgggaagata ctcggcggcg gcttgcccgt gggggcaatc accggccgtg
     5461 cggacgtgat ggaggtgttc accgggctcg gggcgagcaa cggggtgttc tccggcggta
     5521 cgttctcggg caacccgctc accatggagg cgggagcggc cgcggtgggc taccttcgcg
     5581 accaccccga cgtgtaccgt ggtctcgagc aggctggcga gcggctggcg ggggcggtga
     5641 acggcttctg ccaggaacgg cagatcccgg cccagatgaa gtccgtgggg agcatgtttc
     5701 acatgttctt tcagcgggag cccatccgca gcatgcgcga cgtcaccggc gcgagcgtcg
     5761 ccgcggagaa ggcgttctat ctgcacgcgc tgaaccgggg cgttctggtg cccgggaccc
     5821 agcgcgcgtt cctttccgct gctcattccg acgcggaaat tgatactctt atcgatgtgt
     5881 ttcagacatc gcttgccgac gtgcaggcgg agggcctttt ctcggcccga acagacaaag
     5941 gggaaatgcg atgagtcgac tacagaaaat cgagcctgaa gactgggcgc ctgagctcaa
     6001 ggccgcgatc aatcccgaga acctgacccc gctggagcag ggcctgaccc ggttcttcgc
     6061 tcatcggccc gagcttacgc tgggcctgat gcagtttggc ggcgcgctca agaccaagcg
     6121 gacgctccct gacaagctca tcgagctcgt gcggctgcgg gtcgcgttct tcaaccagtg
     6181 ccgcagctgc atggcgatcc gttacacgga cgctctcaac gacggcgtga ccgaggatct
     6241 ggtctgctcg ctggagaaac cggccgaagc cgggaacctc acagatgccg agaaagcggc
     6301 catccggttc ggggagctca tggcgaccga tcatctggcc atcgacgatg ccgtctacga
     6361 cgagctgcgc aagcacttct cggaaagcga gatcgtggag ctggggatgc acgtggcgtt
     6421 cttcgtgggg ttcggcaggc tggcggccac ctggcacatg gtggaagagc tgccggcagc
     6481 gtttcaggac gacagccgtg ccttgagtcc ctggaacgaa gaatccatcg tcgtgcgttg
     6541 agaccggcgc tgccggcccg ctccgggcgg cggcgcgttg cgtccttatc ttcacgcgcc
     6601 cgtcacgggc gcggaaagat catcatggag ccgaaggccg gaaagggtcg gcgtctaggg
     6661 cagtcgtaga cagtaaggag aagtagccgt ggatttcgag ttcagcaagg aagatcaggc
     6721 gttccgggaa gaggtcaggg ccttcctgga agagaaggtc acgccggaca tgcgccaggc
     6781 ggtgcgcaag acgacgacgg tgttcgctga caaggaactc gccatgcgct ggcaggcgat
     6841 tctcgtggag aaagggtggg ccgtgccggc gtggcccgcc gagcacggcg gcaccggatg
     6901 gagcgtcacc cagaagtaca tctggggcga agagtgcgcg cgggcgtccg cccccggcct
     6961 catccctctc gggcttcgca tgctggcgcc cgtgatcttc aagtacggga ccgaggagca
     7021 gaagaactac tacctgcctc gcatgctgtc cggcgagcac tactggtgtc agggctactc
     7081 ggagcccggg tccggatccg atctttccag cctgaaaact tccgcagtca aggacggcga
     7141 cgactacatc gtcaacggca ccaagatctg gacgacccac gcccagtttg ccgaccacat
     7201 cttctgcctg gtgcgcacag acacgggcgt gcgtccccag gcaggcatca ccttcctgct
     7261 gatacccatg gacacgccgg gaatcaaggt ggagccgatc atcaccatgg ccggcgacca
     7321 cgaggtcaac caggtgttct tcgacgacgt gcgagtgccc caggcgaatc gggtagggcc
     7381 ggagaacgac ggctggaacg tggccaagta tctgctcgag ttcgagcgcg gcggcggtgg
     7441 tgcggccgtc cggctcaaag agtcgctcaa ggagctgaga aacatcgcct ccagcgagct
     7501 ggccgacggc gagccgctga tggatgatcc cggcttccgc aaccggatca gcgagctgga
     7561 gatccgcacc tcggcgctcg agttcgcgga gctggcttcc cttgcccgga tgtcccgggg
     7621 cgagtcgccc ggcaccgctt cctccctggc gaagaacacg tccgtggaaa tcgaccaggg
     7681 catccttacc ctgaagctcg aggcggtaca gcattacgcc atgccccacg cgaacaagat
     7741 ctcgctggac ggccacaacg agccgtggcc gggacccgac cacgccgaga cggtcacgcc
     7801 ccgctacctg aacgtgcgcg cggcgtcggt ttacggcggg tccaaggaag tccagaagaa
     7861 catcatcgcc aaggcagtcc tggggctgta accccgcgcc cggcggcggc tatactggct
     7921 cgcttcgccc ggggcccatt cctccgggcc gcttctgggc caaaaggggg cgacgatgag
     7981 cgcaacgaag cgggtgtccc gcggcaaact ctcggtggcc gccgagcttg atgatctggt
     8041 ttctcaacgg atcgcgccgg gcaccggcgt ggatgcggag gcgttctggg caggcttcga
     8101 gtccatcgtc gacgacctcg ctccccgcaa ccgggcgctg ctcgcccggc gggacgagct
     8161 ccaggccacc atcgatgaat ggcacgaaga ccgccgaggc cgtcccctgg acgcgcagga
     8221 gtatcgggcc tttctggagc agatcggcta
//
LOCUS       DBNHLM010000061         2558 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_367109, whole genome shotgun sequence.
ACCESSION   DBNHLM010000061 DBNHLM010000000
VERSION     DBNHLM010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_367109"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="AC16QF_03000"
     CDS             complement(<1..1245)
                     /locus_tag="AC16QF_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR6447550.1"
                     /translation="MKARAFVAAILVLTGASAGARENVAWPAYGGGPGGGHYSQADQI
                     TPDNVAGLEVAWQHRSGDYREGTIRGVEGFSGDAVAASAFIGTPIVVNDTLYYCTPFN
                     RVFALDPQTGAERWMFDPGVKHELEHLTNCRGVSSWTDPLASPDALCAHRIIAPTLDG
                     RIFAVDGKTGKRCEGFGRNGEIDLTEGLTEHGAQEYGITSAPAILNDILVTGSYVTDS
                     IRIDIPSGVVRAYDLRTGAFRWGWNPVPPGTAERDEAGNYRAGTTNVWSTISVDAERN
                     LVIVPTGNSSPDYYGGHRDGDLDYYSSSVVALDGDTGEVVWHYQTVHHDIWDYDLPAQ
                     PTLLDVTIDGKTRPAVVQVTKMGMTFVLDRETGQPLFPVEERPVPQEGAAPGEYLAPT
                     QPFPLKPEPLHKLGISPYDAWGF"
     gene            1389..>2558
                     /locus_tag="AC16QF_03005"
     CDS             1389..>2558
                     /locus_tag="AC16QF_03005"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6447551.1"
                     /translation="MASDQASGASARPDAAHSGPARPLSRRTLFYYGLADMPIQMAGI
                     PVAAFIPNYYGQDLGVSLGAVGTIWLLTRLFDAVSDPLIGWLSDRTDTRWGRRRVWMV
                     AAVPILMLSVYKLFMPDPAAVSAGYLLGWLVALWVGWTMLFIPYYAWAAELSPDYNER
                     TRITGWRSWIGMAANVVSKVVPVIAIYCCAFGGTEETLFLIGTMTLILLPVTVGLTVM
                     KVPEGRDYLPTRIPLARGLKLMWQNAPFKRLVLAFFINQLGSSISTALVVFYIRVVLQ
                     EDAMPIVFLLTYYLFNLSGIPLWVWFSGKVGKHRAWCVGLALFGTAQCGYLLLGPGDF
                     LYMLPIGALTGFLGGSFWVLPNSMTAGVIDVDTLNTGEDRAAWYFAVWSFTTKVGQ"
BASE COUNT          389 a          846 c          836 g          487 t
ORIGIN      
        1 gaagccccac gcatcgtagg gggagatgcc cagcttgtgc agcggctcgg gcttgagggg
       61 gaagggctgg gtaggcgcaa gatactcgcc gggcgccgca ccctcctgag gcaccggccg
      121 ctcctccacg ggaaagagcg gctgcccggt ctcccgatcg agcacgaacg tcatacccat
      181 cttggtcacc tgcaccacgg ccggacgcgt ctttccgtcg atggtgacat ccagcagggt
      241 cggctgcgcg ggcaggtcgt aatcccagat gtcgtgatgc accgtctggt agtgccagac
      301 gacctcgccg gtgtcgccgt ccagcgccac cacggagctc gagtagtagt ccaggtcgcc
      361 gtcccggtgg ccgccgtagt agtcgggcga cgagttgccc gtcggcacga tcaccaggtt
      421 gcgttcggcg tccacgctga tggtggacca gacgttggtg gtgcccgcgc gatagttccc
      481 cgcctcgtcg cgctcggcgg tgcccggcgg tacggggttc caaccccagc ggaacgctcc
      541 ggtgcgcagg tcataggcac gcacgacgcc cgagggtatg tcgatgcgga tgctgtccgt
      601 gacgtaggag cccgtgacga gaatgtcgtt gagtatggct ggcgcgctgg taatgccgta
      661 ttcctgggcg ccatgctcgg tcaacccctc ggtgagatcg atctcgccgt tgcggccgaa
      721 gccttcgcaa cgcttacccg tttttccgtc cacggcgaag atacggccgt ccagggtcgg
      781 ggcgatgatg cggtgggcgc aaagggcgtc gggagacgcc agggggtccg tccaggagct
      841 caccccgcgg cagttggtga ggtgctccag ctcgtgcttc acgcccgggt cgaacatcca
      901 acgctcggcg cccgtctgag gatccagggc aaaaacccga ttgaacggcg tgcagtaata
      961 cagcgtgtcg ttcacgacga tgggcgtgcc gatgaaggcc gaggcggcca ccgcgtcgcc
     1021 gctgaagccc tccacgccgc ggatggtgcc ctctcgatag tcgcccgagc gatgctgcca
     1081 ggcgacctcc aggcccgcca cgttgtccgg cgtgatctgg tcggcctggg agtagtgacc
     1141 gcctccgggt ccgccaccgt aggccggcca ggcgacgttc tcccgagcac ccgcggatgc
     1201 gcccgtaagc accagaatgg cagcgacgaa agctctggct ttcatggtga atcccctccc
     1261 gatcatcgcg cggctccagc ctgcgctgag cctcattcaa ccacccgctc cgccgcagat
     1321 caaaaggaaa acacgttata ctcggcggga cgtggtgcat ccgcttttca gggtgagggg
     1381 agatccattt ggccagcgac caggcgtccg gcgcatcggc acggcctgat gccgcgcact
     1441 ccggcccggc gcggccgctg tcgcgcagaa ccctgttcta ctacgggctc gccgacatgc
     1501 ccatccagat ggcgggcatt cccgtggcgg ccttcatccc caactactac ggccaggacc
     1561 tcggcgtcag cctcggggcg gtcgggacca tctggcttct cacgcgcctg ttcgatgcgg
     1621 tatcggatcc gctgatcggc tggctgtccg accgcacgga tacgcggtgg gggcgccgac
     1681 gcgtttggat ggtggcagca gtgcccatac tcatgctgtc ggtctacaag ctcttcatgc
     1741 cggatcctgc tgcggtgagc gcaggctatc tgctgggctg gctggtggcg ctctgggtgg
     1801 gctggaccat gctgttcatc ccctactacg cctgggcggc cgagctgtct ccggactaca
     1861 acgagcgcac ccgcatcacg ggctggcgtt cctggatcgg catggcggcc aacgtggtca
     1921 gcaaggtggt tcccgtcatc gccatctact gctgcgcgtt cggcggcacc gaggagacgc
     1981 tgtttctcat cggcaccatg accctgatcc tgctgcccgt caccgtgggc ctgaccgtga
     2041 tgaaggtccc cgaaggtcgc gactacctgc ccacccgtat tcccctggcg cgggggctca
     2101 agctcatgtg gcagaacgct ccgttcaagc gcctggtgct ggcgttcttc atcaaccagc
     2161 tgggctcgtc catctccacg gcgctggtgg tgttctacat tcgcgtggtg ttgcaggagg
     2221 acgccatgcc aatcgtgttt ctgctcacct actacctgtt caacttgagc ggcattccgc
     2281 tgtgggtctg gttctcgggc aaggtgggca agcatcgcgc ctggtgcgtc ggcctggccc
     2341 tgttcggcac tgcccaatgc ggctatcttc tgctgggtcc cggcgacttc ctctacatgc
     2401 ttcccatcgg cgcgctcacc gggtttctcg gtggctcttt ctgggtgctc ccgaactcca
     2461 tgacggcggg cgtgatcgac gtggacacgc tcaacaccgg cgaggaccgc gcggcgtggt
     2521 acttcgccgt ctggtccttt accaccaagg tcgggcaa
//
LOCUS       DBNHLM010000062        10223 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_906541, whole genome shotgun sequence.
ACCESSION   DBNHLM010000062 DBNHLM010000000
VERSION     DBNHLM010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10223)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10223)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10223
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_906541"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="AC16QF_03010"
     CDS             <1..596
                     /locus_tag="AC16QF_03010"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: protein motif:HMM:NF006599.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HIR6447552.1"
                     /translation="ARHGVPHAVAPHLINYRANLHLLSELGVEEIVALNTVGGIAKHA
                     RNGRIVLPDQIIDYTWGRAHSFAAEGDLKHVDFTEPYDAALRAELLAAARGQGLDVHD
                     GGVYGCTQGPRFESPAEIDRMDRDGCTVVGMTGMPEAGLARELGIRYASLCLVVNPAA
                     GRGDAIDLDHIAVIAREGMEDVARLLGGFFQGIERGD"
     gene            complement(555..1499)
                     /locus_tag="AC16QF_03015"
     CDS             complement(555..1499)
                     /locus_tag="AC16QF_03015"
                     /inference="COORDINATES: protein motif:HMM:NF022425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsiV family protein"
                     /protein_id="HIR6447553.1"
                     /translation="MSAMSPRRAAATAGLAAALLGQASDAMEAEEPVDLLLKDWYVTE
                     IIVVQRPPVMDFNSAERLLRDRPPALPLAMRTFATDPSQLGKDLDLDLSTRLSLVHPT
                     LALLPPAQDQADVDEGSGAMVAPAIEPVLEPDPLLDVLAMAAALEEQLEARSFQLIPA
                     EELTMSASADRLARRSGFQVLYHGAWLQPVPPRDAPEYLLLQTGARVADRHQLEGVVA
                     VTLGRYLHFQASFSYTEPGMGLEPMTLVLNPDGSGTPARWPVETRPVMIMNQSRRMRS
                     EEVHYLDHPKLGIIVRIDPVPVPEELVAAFDALEEPTE"
     gene            complement(1496..4957)
                     /gene="mfd"
                     /locus_tag="AC16QF_03020"
     CDS             complement(1496..4957)
                     /gene="mfd"
                     /locus_tag="AC16QF_03020"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR6447554.1"
                     /translation="MSPFQPPTPPAVGSRIAWNELHDGAAGLAVGRAAAAHEGLTLMV
                     AESTRQAQRWLDELRFFAPDVHLLHFPDGETLAYDAFSPHQDIISERLSTLRDLPSQR
                     TEREPTLLVVPVQTLLQRVAPRTYLDGHSLSLRTGQHFDLDAQRGRLNAAGYNAAETV
                     SAHGEYAVRGSLMDIYPMGADLPIRIDLFDDEVESLRTFDPDTQRTVEKIDAIRLLPA
                     KEFPLDGNAIARFRSNWHNTFNVDVRRCSVYQDISEGISPNGIEYYLAFFFEELATLF
                     DYLPANTVVIREAGVREAAERFLHDVGVRYESLRHDVERPILPPSDVYLRREELNAAL
                     KAWPQIVINDASARHAVDFATQACGDLEANPRLRDPARGLCEFLDGITQPVLFVADSA
                     GRREVFLEFLNAAGIRPRETADLPAFRDSRLDCAIAVAPVEQGLWTDELVIVTESQVF
                     GHQLEERRARSARVLDPEQIIRNLTELHVGDPVVHIEHGIGRYLGLTTLEIDGAPSEF
                     LAVAYAEDAKLYVPVTSLHLISRYAGAEHAPLHRLGSDAWDKAKRKAAERAHDVAAEL
                     LNIYARRASHSSYRFNDPSADYRRFAAQFPFELTPDQGRAIDEVIADLCSERATDRLI
                     CGDVGFGKTEVAMRAAFLAVQSNKQVAVLVPTTLLAQQHFETFRDRFADWPVQIEALS
                     RLRSESEVEAIAERMASGRIDVIIGTHKLLNPALSFNDLGLVIIDEEHRFGVRQKERL
                     KALRAEVDVLTLTATPIPRTLNMAMSGMRDLSIIATPPAKRLSIKTFVQEKRQHVIKE
                     AVTRELMRGGQVFYVHNEVRTIERAAAELAELVPEARIGVGHGQMPKRQLERVMNDFY
                     HRQLNVLVCTTIIETGIDIPNANTIIMERADKLGLAQMHQLRGRVGRSNRQAYAYLLT
                     PHPKAITKDAVKRLEAIEAAGELGVGFTLATHDMEIRGAGELLGEEQSGQIESVGYSL
                     YMDLLDRAVKAIQDGKTPNLNAPLEPIAQEVNLHTPTLIPEHYLPDVHARLILYKRIA
                     NAADPTELDDLQAEMIDRFGLLPEPLKSLFAVTALKLKTQPLGVVRVDLGESGGRIEF
                     SPDTEVDPLAIVNLVQSHPGTYRLDGATMLRINRTMPDFQARITFAGELLDRLAAHTP
                     NRLVANA"
     gene            5309..7093
                     /locus_tag="AC16QF_03025"
     CDS             5309..7093
                     /locus_tag="AC16QF_03025"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6447555.1"
                     /translation="MKPTNILDPNYFHKVVDCQWACPAHTPVPEYIRLIAQQRYTEAY
                     MLNWDSNVFPGILGRTCDRPCEPACRRVRTEEKPVAICRLKRVAADHKGDISAYLPKV
                     PERKNGKRIALLGAGPASLAVARDLLPFGYEVHLFEKEPMGGGMMRSQIPAFRLPVGV
                     LEEEVDVILDMGVECHFGHEVSSMKEMLDEDFDAYFIGTGAPRGRDLELPGRAEVAEH
                     ISIGIDWLSSVAFGHTHSISGKVIVMGGGNTAMDCCRTAKRLGAESVSVVVRSAFEVM
                     KASEWEKEDAMSEGIPIINNRPPKEFVHENGKLKGVVFECVKPVKGDDGRLRYEPSGE
                     PDVFMEADHILMAIGQENHCPWIERDIGIQFDKWDCPMLDEETLQSTHPKVFFGGDSA
                     FGPENIIWASAHAHKAAISIHLMCQGKDLTADRPPEGVNLISQKMGVHEWSYDSEHDP
                     ARRYLVPHADKRLALADIKMEVELGFDEELGAKEAQRCLNCDVQTVFVDQLCIECDAC
                     VDICPMDCITFTENAEEPDLRRRLTAPAGNVEQDLYVSDELNTGRVMVKDEDVCVHCG
                     LCAERCPTNAWDMQKSIIHIPQLGSYAE"
     gene            7090..8919
                     /locus_tag="AC16QF_03030"
     CDS             7090..8919
                     /locus_tag="AC16QF_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR6447556.1"
                     /translation="MTKRTIVNDFVIRFANVNGSGSASANGMFAKALFRMGIPVATRN
                     IFPSNIQGLPTWFEVRVSERGYLGRREGVDIMVAMNAETFEEDVASLNPGGYLVYDNT
                     KALPASLRRSDVHEVGIPISSMLLSEFSDPKQRSLFKNITYLGALTALLNIEFDVITG
                     MVSTQYKGKDALIGPNIHALELGRDYARDYLECPLPFQVKRADLNGDKIMVDGNTAAA
                     LGALYGGATVCAWYPITPSTSVAEEFERFANRMRIDGQSGQRKFAIIQAEDELSAIGI
                     VIGAAWNGARSFTATSGPGISLMTEFLGLAYFAEIPAVVFDIQRSGPSTGMPTRTQQG
                     DLISSAYASHGDTKHVLLFPSTPRECFDHAALALDLADRLQTPVIVMSDLELGMNDSI
                     SEPFEWDDERRYDRGKVLDREQLDSLERFGRYLDVDGDGIGYRTLPGTHPDKGAFFTR
                     GTSRDEFAAYTESPDVYVANMERLSAKWRTAARLVPSAEIESNGERAGIIYYGTSVAP
                     MREALDMLEEEGIRLDTMRITAFPFGAEVYDFIAAHDTVFVVDQNRDAQMKVLLVTEG
                     ELDPAGLVSVLYYGGLSISADTIQKQVSDYFTEQRLPRLKEVK"
     gene            8919..9974
                     /locus_tag="AC16QF_03035"
     CDS             8919..9974
                     /locus_tag="AC16QF_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIR6447557.1"
                     /translation="MTFVVKPRVHHPKLPVNEIGLTRRDYEGPVSTLCAGCGHDSVSA
                     AVVQACAELSLPPHKFAKVSGIGCSSKTPSYFLSKSHGFNSVHGRMPSVATGANLANR
                     ELICIGISGDGDSASIGLGQFAHIVRRRINMVYIVDNNGTYGLTKGQFSATNDKGSTN
                     KKGVPNLYEPIDLVSMALQLGASFVARSFSGDKRQLVPLIKAALTHNGFAFLDVISPC
                     VAFNNHEGSTKSYDYVRGHSENINVLDFIPEGVEITADYEPGTTEDVRMPGGSILRLR
                     KLDGAYDPHDRVEALHYVQQQQAEGEVVTGLLYVDPDASDCHEIMDTVQRPLNELNED
                     DLCPGDDALEAVNESFR"
     gene            10084..>10223
                     /locus_tag="AC16QF_03040"
     CDS             10084..>10223
                     /locus_tag="AC16QF_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR6447558.1"
                     /translation="MPSLDDYFPDWKEREALAEAMIPIIGRLYRNNVVTYCYGRALYN
                     QSV"
BASE COUNT         1839 a         3144 c         3372 g         1868 t
ORIGIN      
        1 tggcgcgaca cggggttccg cacgcggtcg ccccgcacct gatcaattat cgggccaatc
       61 tgcacctgct ctccgagctg ggcgtcgagg agatcgtcgc gctgaatacc gtcggcggga
      121 tcgccaagca cgcgcgcaac gggcgcatcg tgctgcccga tcagatcatc gactacacgt
      181 ggggccgtgc ccacagcttc gctgcagagg gcgacctcaa gcacgtggat ttcacggagc
      241 catatgatgc tgcgctgaga gccgagctgc tggctgctgc ccgggggcag gggctggacg
      301 tgcacgatgg cggcgtctat gggtgcactc agggcccgcg tttcgagtcg ccggctgaga
      361 tcgatcggat ggaccgggac ggttgcacgg tggtcggcat gacgggaatg cccgaagccg
      421 ggctcgcccg ggagctggga atccgttacg cgagcttgtg cctggtggtg aacccggctg
      481 ccggtcgggg agatgccatc gacctggacc acatcgcggt cattgcgcgt gagggcatgg
      541 aggacgtggc gcgcctactc ggtgggttct tccagggcat cgaacgcggc gactagttct
      601 tccggaacgg gcaccggatc gatgcgcacg atgatgccca gcttcggatg atccaggtaa
      661 tgaacctcct cgctgcgcat gcgtcgactc tggttcatga tcatcaccgg gcgagtttca
      721 acgggccatc gcgccggcgt gcccgaaccg tccgggttga gcaccaaagt catcggctcg
      781 agccccatgc cgggctcggt gtaagagaag ctggcctgaa agtgcaggta ccgcccgagg
      841 gtgacggcca cgaccccttc gagctggtgg cggtcggcca ctctggcgcc ggtctgcagc
      901 agcaggtatt cgggcgcatc gcgcggcgga accggttgca gccaggcgcc gtgatagagg
      961 acctggaagc cgcttcgccg ggcgagccgg tcggcgctgg cggacatggt cagctcctcg
     1021 gccgggatga gctgaaagct gcgggcctcg agttgctcct cgagcgcggc ggccatggcc
     1081 agcacatcga ggagggggtc gggttcgagc accggttcga tggctggagc aaccatcgcc
     1141 ccggatccct cgtcgacgtc tgcttggtcc tgcgcagggg gtagcaaagc cagggtcgga
     1201 tgcacgaggc tcaggcgggt cgacagatcc agatccaggt cttttccgag ctgggaggga
     1261 tcggtggcga acgtgcgcat agccaggggc aacgccggcg gacggtcccg caggagccgt
     1321 tcggcggagt tgaaatccat gaccgggggg cgctgcacga cgatgatctc cgtgacgtac
     1381 cagtctttga gcagaagatc gacaggttct tcggcctcca tggcgtcgga tgcctgcccg
     1441 aggagcgcgg cagcaagacc cgccgtggcg gccgctcttc gggggctcat ggcgctcatg
     1501 cgttggccac cagccggttc ggtgtgtgtg ccgccaggcg atctagaagc tcgcctgcga
     1561 acgtgattcg cgcctgaaag tccggcattg tccggttgat cctcagcatc gtcgcgccgt
     1621 ccagccggta cgtcccggga tggctctgta cgaggttcac gatggccagc ggatcgacct
     1681 cggtgtcggg gctgaactcg atccgcccgc cgctttcgcc gagatccacg cgaacgacgc
     1741 ccagcggctg ggttttcagc ttcaaggccg tgacggcaaa cagggatttc agcggctcgg
     1801 gcaggagccc gaagcgatcg atcatctccg cctgcagatc gtctagctcc gtcgggtcgg
     1861 cggcgttggc gatgcgcttg tagagaatca gccgggcgtg cacgtcgggc aggtaatgct
     1921 ccgggatgag cgtcggcgtg tgcaggttga cctcctgagc gatgggttcc agcggggcat
     1981 tgaggttcgg cgtcttgcca tcctgaatgg cttttacggc ccggtccaga aggtccatgt
     2041 agagggaata tcccacagac tcgatctgtc ccgactgctc ctctccaagc agttcgcctg
     2101 cccctcggat ttccatgtcg tgggtggcca gagtgaaacc gacgccgagc tcgccggccg
     2161 cctcgatggc ttccaggcgt ttgacggcgt ccttggtgat cgccttggga tgcggcgtca
     2221 gcaggtaggc gtacgcctgc cgattcgatc ggccgacccg gccgcgaagc tgatgcatct
     2281 gcgccagacc cagcttgtcg gcacgctcca tgatgatggt gttggcattc gggatgtcga
     2341 tgcccgtctc gatgatggtc gtgcagacga gcacgttcag ctggcgatgg tagaagtcgt
     2401 tcatgacgcg ctcgagctgg cgcttgggca tctggccatg accgacgcca attctggcct
     2461 cggggaccag ctccgccagc tctgcggccg ccctttcgat ggttcgcact tcgttgtgca
     2521 cgtagaagac ctggccgccg cgcatcagct cgcgcgtcac ggcttccttg atgacgtgct
     2581 ggcgcttttc ctgcacgaac gttttgattg aaagccgttt ggccggcggc gtggcgatga
     2641 tggagagatc gcgcatgccg ctcatggcca tgttcagcgt gcggggaatg ggggtggccg
     2701 tcagggtcaa tacgtcgacc tctgctcgca aagccttgag ccgctccttc tgacggacgc
     2761 cgaagcggtg ctcctcgtcg atgatcacca gacccagatc gttgaaacta agcgcgggat
     2821 tgagcagctt gtgggtgccg atgatcacgt cgatccggcc cgacgccatt cgctcggcga
     2881 tggcttcgac ctcgctttcc gagcgaagcc gtgacagcgc ttcgatctgc acaggccagt
     2941 cggcgaagcg gtcgcggaag gtctcgaaat gctgctgggc cagaagcgtc gtgggcacta
     3001 gcacggccac ctgcttgttg ctctgcaccg ccagaaaggc ggcgcgcatg gccacctcgg
     3061 tcttgccgaa gccgacgtcg ccgcagatga ggcggtcggt cgcccgctcg gagcataggt
     3121 cggcgatgac ctcgtcgatg gcgcggccct gatcgggcgt cagctcgaag ggaaactgtg
     3181 ccgcgaagcg gcggtaatcg gcgctcgggt cgttgaagcg gtagctggag tggctggccc
     3241 ggcgggcgta gatgttcagg agctcggcgg cgacatcgtg ggcacgctct gctgccttgc
     3301 gcttggcctt gtcccaggca tccgatccca ggcgatgcag cggcgcgtgc tctgcgccag
     3361 cgtagcggct gatcaggtgc agcgatgtga ccggcacgta aagcttcgcg tcttcggcgt
     3421 aggccacggc gagaaactcg ctcggcgcgc cgtcgatctc cagggtggtc aggccgagat
     3481 agcgaccgat gccatgctcg atgtggacca cggggtcgcc aacgtgcagc tcggttaggt
     3541 tcctgatgat ctgttccggg tccagcacgc gagcgctccg ggcgcgtctc tcctcgagct
     3601 gatgcccgaa aacctggctc tcggtaacga tcaccagctc gtcggtccac agaccctgct
     3661 ccacgggggc gacggcgatt gcgcagtcga gcctcgaatc tcggaacgcg ggcaggtcgg
     3721 cggtctctcg cggacgtatg ccggccgcgt tcaggaattc caggaacacc tcgcggcgcc
     3781 cggccgagtc ggccacgaaa agcaccggct gggtgatgcc gtcgagaaac tcgcacaggc
     3841 cgcgcgcggg gtcccgcagc cggggattgg cttccagatc tccgcaggcc tgagttgcga
     3901 agtcgaccgc gtgacgggcg ctggcgtcgt tgatgacgat ctgcggccag gccttcaacg
     3961 cggcgttgag ctcctctctg cgcaggtaga cgtcggacgg gggaagaatg ggtcgctcca
     4021 cgtcgtgacg caggctctcg tagcgaacgc ccacgtcgtg aagaaaccgc tcggcggctt
     4081 cccgaacgcc ggcctcccgg atgaccacgg tgttggctgg caggtagtcg aagagcgtag
     4141 ccaactcttc gaagaagaag gccaggtagt actcgatgcc gttcggggag atgccctcgc
     4201 tgatgtcctg gtagacgctg catcgccgca cgtcgacgtt gaacgtgttg tgccagttgc
     4261 tccggaatcg tgcgatggcg ttgccatcca agggaaattc ctttgcgggc agcagccgaa
     4321 tcgcgtctat cttctcgacg gtgcgctggg tgtcggggtc gaacgtccgc aggctttcca
     4381 cctcgtcgtc gaacaggtcg atacggatgg gcagatccgc tcccatgggg tagatgtcca
     4441 taagcgaacc gcgcacggcg tactccccgt gagcgcttac cgtctctgcc gcgttgtacc
     4501 ccgccgcgtt gagccggccg cgttgcgcat ccaggtcgaa gtgctggccc gtgcgaagtg
     4561 aaaggctgtg gccgtcgagg taggtccgcg gggctacccg ctggagaagg gtctggacag
     4621 gcaccacgag cagggtgggt tcccgttcgg tgcgctggga cggcaggtcg cgcagcgtgg
     4681 acagacgttc ggagatgatg tcctggtgcg gcgagaaggc atcgtaagcc agggtctcgc
     4741 cgtcgggaaa atgcagcagg tggacgtccg gggcgaagaa tcgaagctcg tccagccagc
     4801 gctgggcctg ccgcgtgctc tcggcgacca tcagcgtcag cccctcgtgg gcggcggcag
     4861 cgcggcctac ggcaagcccc gcagcaccgt cgtgaagttc gttccaggcg atgcgggaac
     4921 ccacggctgg cggcgttggc ggttggaatg gcgacagctc gttggccatc agagttctgg
     4981 gcggttgacc atagcggcgc gtattctact cgggaattgg cggttccggt tggtttcccc
     5041 cttccgacgc ctcgaggtcg tcgctgacgc cggcgctggt cgctacgcgg cctcagacgt
     5101 gctgggcaac gtgtcacacc aggcggccaa tttggttggc aagccacgaa aaataaggtt
     5161 agagttcgcg actgtccagg ctcggcctgg ctatatggat ggcccagcag tcggccggat
     5221 gaacggtttg ccctgtcgcc gcaaattagc gtaaccaaag gagctgggag gcgtagccgt
     5281 gcatgcctgc cggcgagtcg tcgtacgcat gaaacctaca aacatcctcg accccaacta
     5341 tttccacaag gtagttgact gtcagtgggc ttgcccggca cacacgccgg tgccggagta
     5401 catccggctg atcgcgcagc agcgatacac cgaagcctac atgctcaact gggactccaa
     5461 cgtgttccca ggaattcttg gccgcacctg cgaccgtccc tgcgaaccgg cgtgccggcg
     5521 ggtgcgcacc gaggaaaaac ctgtcgccat atgccgcctc aagcgcgtcg ccgcggacca
     5581 caagggcgac atcagcgcct atctgccgaa ggtcccggag cgcaaaaacg gaaagcgtat
     5641 tgccctgctc ggcgcgggcc ctgcttccct ggcggtggcc agagatctgt tgcctttcgg
     5701 ctacgaggtg catctgttcg agaaagagcc catgggcggg ggcatgatgc gcagccagat
     5761 acccgcgttt cgcctcccgg tgggcgtgct cgaagaagag gtcgatgtga ttctcgacat
     5821 gggcgtcgag tgccacttcg gtcacgaggt ctcgagcatg aaagagatgc tcgatgagga
     5881 tttcgacgcc tacttcattg gtaccggcgc gcctcggggt cgagatctcg agctgccggg
     5941 cagagcggaa gtagccgagc atatctccat cggcatcgat tggctctcca gcgtcgcgtt
     6001 cgggcacacc cactcgattt ccggcaaggt catcgtcatg ggcggcggca acaccgccat
     6061 ggattgctgc cgcacggcca agcgcctggg ggccgagtcg gtctcggtag tggtgcgttc
     6121 cgcgttcgag gtgatgaagg cctcggagtg ggaaaaagaa gacgccatga gcgagggcat
     6181 accgatcatc aacaatcggc cgcccaagga gttcgttcac gaaaacggca agctcaaggg
     6241 cgtggtgttc gagtgcgtca agccggttaa gggtgacgac ggtcgcctgc gctacgagcc
     6301 cagcggggag cccgacgtct tcatggaggc ggaccatatc ctcatggcca tcggccagga
     6361 gaaccactgt ccctggatcg agcgggacat cggtatccag ttcgacaaat gggattgccc
     6421 catgctggac gaggaaacgc tgcagtccac tcaccccaag gtcttctttg gcggcgactc
     6481 ggcgttcggg ccggagaaca tcatctgggc ctctgcccat gctcacaagg cggcgatctc
     6541 catccatctc atgtgccagg gcaaggatct cacggccgac cgtccgccgg aaggcgtaaa
     6601 cctcatcagc cagaaaatgg gcgttcacga gtggagctac gacagcgagc acgatccggc
     6661 ccggcgctac ctggtgcccc atgcggacaa gcgtctggcg ctggccgaca tcaagatgga
     6721 ggtcgagctc gggttcgacg aggagctcgg cgcaaaggaa gcgcaacgct gcctcaactg
     6781 cgacgtccag accgtgtttg tcgaccagct gtgcatcgag tgcgacgcgt gcgtggacat
     6841 ctgcccgatg gactgcatca ccttcacgga aaacgccgag gagcccgatc tgcgccgccg
     6901 gctgaccgcg ccggccggca acgtcgagca ggacctctac gtatctgatg agctcaacac
     6961 cggtcgtgtc atggtgaaag acgaggacgt ctgcgtgcac tgcgggctct gtgcagaacg
     7021 ctgccccacg aacgcctggg acatgcagaa gtccatcatc cacattccgc agctgggctc
     7081 gtacgcagaa tgaccaaacg caccatagtc aacgacttcg tcattcgttt cgccaacgtc
     7141 aacgggtcgg gctcggccag cgccaacggc atgtttgcaa aggccctgtt ccggatgggc
     7201 atcccggttg ccacgcggaa catatttccg tcgaacattc agggcctgcc gacctggttc
     7261 gaggtcaggg tgagcgagcg tggctacctg ggtcgccgcg aaggtgtgga catcatggtc
     7321 gccatgaatg ccgagacctt cgaagaagac gttgcgagcc tgaaccccgg cggttacctg
     7381 gtgtacgaca ataccaaggc gctgccggca tcgcttcggc gcagcgacgt gcacgaggtg
     7441 gggattccca tatccagcat gctgctcagc gagttcagcg acccgaagca gcgcagcttg
     7501 ttcaagaaca tcacctacct gggggcgctg acggcgctcc tgaacatcga gttcgacgtg
     7561 atcaccggca tggtgtccac ccagtacaag ggcaaagacg cgctcatcgg cccgaacatc
     7621 cacgcgctcg agctcggccg ggactatgcc cgggactatc tggagtgtcc gttgccgttc
     7681 caggtcaaac gcgctgactt gaacggcgac aagatcatgg tggacggcaa cacggccgcc
     7741 gcgctcggag ccctctacgg cggcgcgacg gtgtgcgcct ggtacccgat cacgccatcg
     7801 acctcggtgg cggaggagtt cgagcgcttt gccaaccgga tgcgtatcga cggccaatcc
     7861 ggccagcgca agttcgcgat cattcaggca gaggacgagc tgtcggccat cggcatcgtc
     7921 atcggtgcgg cgtggaacgg cgcccgttcc ttcaccgcga ccagcggccc gggcatctcg
     7981 ctgatgacgg agtttctcgg gcttgcctac tttgccgaga ttcccgccgt cgtgttcgat
     8041 atccagcgtt ccggcccgtc cacgggcatg cccacccgca cgcagcaggg ggatctgatt
     8101 tcctcggcct acgcttctca cggggacacc aagcacgtgc tgctgtttcc ctcgacgccg
     8161 agggagtgct tcgaccatgc cgcgctggcg ctcgacctgg ccgaccggct ccagacgccc
     8221 gttatcgtca tgagtgatct ggagctcggg atgaacgaca gcatctccga gcctttcgag
     8281 tgggatgacg aacgtcgcta cgaccggggc aaggtgctgg atcgggaaca gctggattcg
     8341 ctggagcggt tcggccgtta cctggatgtg gacggggacg gcatcgggta ccggacgctt
     8401 cccggaaccc atccggacaa aggagcattc ttcaccaggg gcacgtcgcg tgacgagttc
     8461 gccgcgtaca cggaatcccc ggatgtctac gtcgccaaca tggagcggct gagcgcaaag
     8521 tggcgaacgg cggccagact ggtgccttcg gcggagatcg agtcgaacgg cgaaagggcg
     8581 ggcattatct actacggaac ctccgtggcc cccatgcgcg aggccctgga catgctggag
     8641 gaggagggaa tccgcctgga taccatgcgc atcaccgcgt tccccttcgg tgcggaggtg
     8701 tacgatttca tcgcggccca cgatacggtt ttcgtcgtgg atcagaatcg cgatgcgcag
     8761 atgaaggttc tgctggtgac cgagggtgag ctcgatcccg ccggcctcgt ctcggtcctt
     8821 tactacggcg gtctttccat atcggccgac acgatccaga agcaggtttc cgattacttc
     8881 acggagcagc ggctgccgcg cctcaaggag gtcaagtaat gactttcgtt gtcaagccga
     8941 gggttcatca cccgaaactg cccgtcaacg agatcgggtt gacccgacgc gactacgaag
     9001 gcccggtttc gacgctctgc gccggctgcg gccacgactc cgtgagcgct gccgtcgtgc
     9061 aggcctgcgc ggagctgtcc cttccgcccc acaagttcgc caaggtttcc gggattggct
     9121 gctcctccaa gaccccgagc tacttcctca gcaaatccca cggtttcaat tccgtgcacg
     9181 ggcgcatgcc gtcagtggca acaggcgcca acctggccaa ccgggagctc atctgcatcg
     9241 gcatctcggg cgacggggat tcggcctcca tcgggctcgg gcagtttgcc cacatcgtca
     9301 ggcgtcggat caacatggtc tacatcgtcg ataacaacgg cacttatggt ctgaccaagg
     9361 gacagttctc ggcgacgaac gacaaggggt ccacgaacaa gaagggcgtc ccgaatctct
     9421 acgagcccat cgatctcgtc agcatggccc ttcaactggg cgcgagcttc gttgcccgaa
     9481 gcttttcggg cgacaagcgc cagctggtgc cgctgatcaa ggcggcgctc actcacaacg
     9541 ggttcgcctt tcttgacgtg atctcgccgt gcgtggcgtt caacaatcac gagggatcca
     9601 cgaagagcta cgattacgtg cgcgggcaca gcgagaacat caacgtcctg gatttcatac
     9661 ccgagggtgt ggaaatcacg gccgactacg agcccggcac gaccgaggac gtccgcatgc
     9721 ccggaggctc gatcctccgg ctgcgcaagc tggatggcgc ttacgatccc catgatcgtg
     9781 tcgaagccct gcactacgtc cagcagcagc aggccgaagg ggaagtggtc acgggccttc
     9841 tttacgtgga ccccgacgct tccgactgtc acgagatcat ggatacggtg cagcgccccc
     9901 tgaacgagct caacgaagac gatctctgcc cgggcgacga tgcgctcgag gccgtgaacg
     9961 aatccttccg gtaagccctg gtttccttgc gccccagggg gcaaattttg ataatacgcg
    10021 cggctccaag ccgggcccta accccggctg aaaatcgtta acagggaggg agaacctgtg
    10081 gcattgccga gtctcgacga ctacttcccg gactggaagg agcgcgaagc gcttgctgag
    10141 gcgatgatcc ccatcatcgg gcgtctgtat cgcaacaacg tggtcaccta ctgttacggc
    10201 cgggcgctct acaaccagag cgt
//
LOCUS       DBNHLM010000063         7214 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_547578, whole genome shotgun sequence.
ACCESSION   DBNHLM010000063 DBNHLM010000000
VERSION     DBNHLM010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_547578"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="AC16QF_03045"
     CDS             <1..388
                     /locus_tag="AC16QF_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6447559.1"
                     /translation="ADINGDGTVTDNSDIPLQRAPEWTIGAGLSYDAQLGNMGTAVFT
                     ADFSHVAKQNNLASGVPKGEIGSYELLDMSVTWRAPSERYYATFYVKNVTDEVYQASI
                     TAVGALFDTNLISAPRRWGLTVGFDL"
     gene            complement(476..1651)
                     /locus_tag="AC16QF_03050"
     CDS             complement(476..1651)
                     /locus_tag="AC16QF_03050"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6447560.1"
                     /translation="MSREQLIEMTRHAIDHAHAGTIDQEPDVMKIPAANYYDPDRFKV
                     EVKQIFRRMPLVLAPTAEIKNPGDYKAMDAAGMPVLLTRGQDGQVRAFINSCSHRGTA
                     LVIDETGNRRKFTCPYHGWTFDQQGTLVGIASPDDFGEIDRSAYGLTPLPVLEKAGLI
                     WCTLDPESTLDIETFLSGYDDMLANFGFESWHLFDKRTIRGPNWKIAYDGYMDLYHLP
                     VLHRETFGADRSNKALYFPWGPHQRVISPNRYGPLENLPEDKWPTEMMMVGVWTIFPH
                     ISIASFDGGGRSVMLSQLFPGSSPGESYTTQYYLMEHEPNEEQAKEAEAQFKLLEYVV
                     EVEDYGTGLRQQKALEAGGRDHVLFGRNEGGGHRFHGWVDKLIAAKDDELNRMFASV"
     gene            complement(1692..2606)
                     /locus_tag="AC16QF_03055"
     CDS             complement(1692..2606)
                     /locus_tag="AC16QF_03055"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6447561.1"
                     /translation="MSASPLRNVTTEEIESFHRDGAALIKGVLADEWVSLIARGLDYA
                     DAHPDGMSEGVEQPLRIDQFPAARSPDLRRAIDASPIAEIVGVTLDSPVRFYMDQMFI
                     KPAGELVPTPWHQDTCYYNVEGHDLIRAWVCVDPVPREASLEVVRGSHLWNVTYRTVA
                     GRSEAEDPERAERAARAYAAGEAFIGPESHERWSYGDAYLDESLPPIPDVEGHRDSFE
                     VLGWDYEPGDVLLFHGNILHGARGGVTLTHPRRSHASMWAGRDVRYIHRRGQVVPDPA
                     ALYALEPRDGQLLGDFPDVFPLAWSPKH"
     gene            complement(2606..3241)
                     /locus_tag="AC16QF_03060"
     CDS             complement(2606..3241)
                     /locus_tag="AC16QF_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6447562.1"
                     /translation="MNTVPATAASVALDPLTQTLIDAAEACFVRHGVAKTTMSHVAEE
                     AGVSRTTLYKRFAVMEEVLQAVFIREFDRFEARLAGNLEPIDDPGERLVEIVVAIAEN
                     VPENAGLARLLDGSRTRTENMALAAGRRALNDRVIDMIGDPLDGLARAGVLRGDVERG
                     ELIEWIRRVVNSLAVFPQPRSRSSAERRRFVARFLLPSITAAHDLPPMEVC"
     gene            complement(3238..4968)
                     /locus_tag="AC16QF_03065"
     CDS             complement(3238..4968)
                     /locus_tag="AC16QF_03065"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6447563.1"
                     /translation="MHLDLVIRNGMLIDGRRTPSYRADVGIRDGKVAVIGRIAPGSGD
                     RELDADGLIVAPGVVDLHTHYDSQIFWDPWCRISGQHGVTSVVVGNCGFGFAPVRPEH
                     RDRAMLTMARNEAVPLECMQQGMPWDWTTYPEFLDSLDRTPKGVNVLSYVGLNPLMVW
                     LTGSVEEAKGRPLTRAEKAEAERLLAEAMDAGACGWSAQFIGADTVQRDHDGTPMITD
                     LMSREDIEFFAAALGRLGRGGIQMIGPPKMADVLAAASGRPVIYNVLAARSDQHGSPA
                     ESYKDVIAWLARANAEGNRIIAHAVTCAIDYQFTMEDWNLFDSSPLWRELTIGTVPER
                     KAKMMDAQRRAALHEEWAAGKAPIAGGGTEQREERIGVKFQDLVLAYVTEEGEHLRSW
                     EGHTVAEIAAGMGVHPIDAFLDVSIACDLKAGWQSEPRTIDPQVMRDIANCPYAVPGL
                     SDGGAHTKFLTTGTYPTEFLATWVRDNGVMDLEEAHWRLSAYSAQTAGLTDRGFIAEG
                     MPADIVVYDLQALELLPSERVADWPGGAWHLDRRANGWRWTIVNGAVTFADGVCTGAT
                     PGRLLRHGHA"
     gene            5142..5873
                     /locus_tag="AC16QF_03070"
     CDS             5142..5873
                     /locus_tag="AC16QF_03070"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6447564.1"
                     /translation="MQEFHVNVATPDGEMECFAAHPDGEGPFAAVILYMDAPGIREEL
                     RDFCRRIAGQGYFCLLPDLYYREGRVRFDLRKGEEELKRMFAVGSRLTVEAVMRDTGG
                     MLAYLEDNPLARSPTGCIGYCMSGQFVVAAAGTFPGEIAAAASLYGTRIVTDKPDSPH
                     KLAERMKGELYLGFAEHDHWVEDFVIPKLIEELDQHGVKYELEVHPGTEHGFCFPERP
                     SYVEAAAEKVWDTVFELFDRRLNAR"
     gene            5896..6546
                     /locus_tag="AC16QF_03075"
     CDS             5896..6546
                     /locus_tag="AC16QF_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6447565.1"
                     /translation="MKGLTERQLKRRERILAAARKLITDRGYDGLTMRDLAAESGVAP
                     KTLYHQFHSKENLLRLAVEERYRYLYQIIDDEQIEHGIDRLFFIIDTVASTTENNLAY
                     AKALRPLLTSETSSTFAAIRMNTYRKAIDQIEAEGEVVDWIDVDILAAVVYRQVNPIY
                     LAPWYKETPWATVARIAKLDICLILAAVTTGYTQTTAEKTIRDLQKQLKRSGSRYI"
     gene            complement(6553..>7214)
                     /locus_tag="AC16QF_03080"
     CDS             complement(6553..>7214)
                     /locus_tag="AC16QF_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="membrane-bound PQQ-dependent dehydrogenase,
                     glucose/quinate/shikimate family"
                     /protein_id="HIR6447566.1"
                     /translation="DALEAMTTGPIYTPISVNKETPLYPSVIGGQNWGAPAVDPERQI
                     MVANTHHVAMILNLIPRDQCDGTENFPQEGSPYCATLRPFLSRFGAPCTKPPWGTLSA
                     VNLSTGDIEWQVPLGNLKYQAFWPFTMMKGGVQMGGPTITSAGLIFIAASADRHVRAF
                     DLDTGEELWADEMPTTGNAVPMTYVSGGRQYVVIAAGGHFTSPSPPADYLIAYALPEK
                     D"
BASE COUNT         1290 a         2419 c         2249 g         1256 t
ORIGIN      
        1 cgccgacatc aacggcgacg gcaccgtcac cgacaactcc gacatccccc tgcagcgcgc
       61 cccggaatgg accatcggcg cggggctgtc ctacgacgca cagctgggca acatgggtac
      121 ggccgtcttc accgcggact tcagccacgt ggccaagcag aacaaccttg cctcgggtgt
      181 gccgaaaggc gagatcggct catacgagct cctggacatg agcgtcacct ggcgcgcgcc
      241 cagcgagcgg tattacgcca cgttctacgt gaagaacgtc acggacgagg tttaccaggc
      301 gtcgattacc gccgtcggcg cgctgttcga cacgaacctc atcagcgccc cgcgccgctg
      361 gggactgacc gtgggtttcg acctgtaaga ctgggtcgca actcgtaaga aaaagggcac
      421 ctcatgaggt gccctttctt tacccgcgct gtgattgcgc cagtcgaagg ctcgttcaga
      481 ccgacgcgaa catcctgttc agctcgtcgt ccttggcagc gatcagctta tccacccagc
      541 cgtgaaagcg gtggccgccc ccttcgttgc gaccgaacag aacgtggtcg cggccgcccg
      601 cttccagcgc cttctgctgc cgcaggccgg tgccgtagtc ctccacctcc accacgtact
      661 cgagaagctt gaactgcgcc tcggcctctt tagcctgctc ctcgttcggc tcgtgctcca
      721 tgaggtaata ctgggtggtg taggactccc ccggggacga tcccgggaag agctgggaga
      781 gcatgacgct gcggccgccg ccgtcgaagg acgcgatgga aatgtggggg aagatggtcc
      841 acacgcccac catcatcatc tccgttggcc acttgtcctc gggcaggttt tccaaaggcc
      901 cgtaccggtt cggggagatc acccgctggt gcgggcccca cggaaaatag agggccttgt
      961 tggagcggtc ggccccgaag gtctcccgat gcagcacggg caggtggtag aggtccatgt
     1021 agccgtcgta ggcgatcttc cagttgggac cgcggatggt gcgcttgtcg aacaggtgcc
     1081 acgactcgaa gccgaagttg gcgagcatgt cgtcgtagcc ggagagaaac gtctcgatgt
     1141 ccagcgtgct ctcggggtcc agcgtgcacc agatgagccc cgccttctcc agcacgggca
     1201 gcggggtaag cccataggcg gatcggtcga tctcgccgaa atcgtcaggg gatgcgatgc
     1261 ccaccagcgt gccctgctga tcgaacgtcc agccgtgata gggacaggtg aatttccggc
     1321 ggttgcccgt ctcgtcgatc accaaggccg tgccccggtg actgcagctg ttgatgaatg
     1381 cgcgcacctg gccgtcttgc ccgcgggtga gcagaacggg catgccggcc gcgtccatgg
     1441 ccttgtagtc gccgggattt ttgatctccg ccgtgggcgc gagcacgaga ggcatgcggc
     1501 ggaatatctg cttcacctcg accttgaagc ggtccgggtc gtaataattg gctgccggaa
     1561 tcttcatcac gtccggttcc tgatcgatcg tcccggcgtg ggcgtgatcg atggcgtgcc
     1621 gcgtcatctc gatcaactgc tctctggaca tctgcttacc tccctcgctt tcgaaatctt
     1681 accctattga actagtgctt cgggctccac gccagcggaa acacgtccgg aaaatcgcct
     1741 aacagttgcc cgtccctcgg ctccagcgcg tagagcgcgg ccggatcggg caccacctgc
     1801 cctcgacggt gaatatagcg cacatcgcgt cccgcccaca tggacgcatg agaacggcgc
     1861 gggtgcgtca gcgtcacccc acctcgggca ccgtgcagga tgttgccgtg aaacagcagg
     1921 acgtcgcccg gctcgtaatc ccagccgagc acctcgaacg agtcccgatg cccctccacg
     1981 tcgggaatcg gtggcaggct ctcgtcgagg tacgcatcgc cgtaggacca ccgctcgtgg
     2041 ctctccggcc cgatgaaggc ctcccccgcg gcgtaggcgc gggccgcacg ctcggcacgc
     2101 tcggggtctt cggcctcgct gcgtccggcc acggtccggt aggtgacgtt ccacaggtgc
     2161 gatccacgca ccacttccag gcttgcctcc cgcggcaccg ggtcgacgca cacccaggcg
     2221 cgaatcagat cgtggccctc gacgttgtag tagcaggtat cctgatgcca cggggtaggc
     2281 accagctctc ccgccggctt gatgaacatc tgatccatgt agaaccgcac cggcgagtcc
     2341 agcgtcacgc cgacgatctc ggcaatgggc gaggcgtcga tggcgcggcg caggtcagga
     2401 gagcgtgctg ccggaaactg gtcgatccgc aggggctgct cgacgccttc cgacatgccg
     2461 tccgggtgcg cgtccgcgta gtcgagcccg cgcgcgatca gcgataccca ctcatcggca
     2521 agcacccctt tgatgagagc ggcgccgtcg cggtgaaagg attcgatttc ttccgtcgtc
     2581 acgtttctga gcggcgaagc ggacatcagc aaacctccat gggtggcaga tcgtgagcgg
     2641 cagtgatgga aggaagcaga aagcgggcga cgaaccgccg gcgctccgcc gacgacctgc
     2701 tcctgggctg gggaaatacc gccaaagagt tgaccacacg ccggatccac tcgatcagct
     2761 cgccccgttc cacgtcccca cgcagcaccc cggcgcgcgc aagcccatcg aggggatcgc
     2821 cgatcatatc gatcacccga tcgttgagcg cacgccgccc ggcggcaagc gccatgttct
     2881 ccgttctcgt cctcgagccg tcgagcaagc gggccaaccc cgcgttttcg ggcacgttct
     2941 cagcaatcgc cacgacgatc tccaccagcc tctcaccggg atcatcaatc ggttcgaggt
     3001 tgcctgcaag ccgcgcctcg aaccgatcga actcgcgaat gaagaccgcc tgcagcacct
     3061 cctccatgac ggcaaaccgc ttgtagagcg tggtgcgcga aacgcccgcc tcctcggcca
     3121 cgtgcgacat ggtcgtcttc gccactccgt ggcgcacgaa gcaggcctcc gctgcatcga
     3181 tcagggtctg cgtgagcggg tccagcgcga ccgaagccgc cgtggccggc acggtgttca
     3241 tgcgtgaccg tgcctgagca ggcgcccggg cgtcgcgccg gtgcacacgc cgtctgcaaa
     3301 ggtgaccgcg ccgttgacaa tcgtccagcg ccagccgttc gcgcgccggt cgaggtgcca
     3361 cgcgccgccg ggccaatccg caacgcgctc ggacggcaac agctccagcg cctgcagatc
     3421 gtagaccacg atgtccgcgg gcatgccctc cgcgatgaac ccccggtccg tcagccccgc
     3481 cgtctgcgcg gagtaggcgg agaggcgcca gtgcgcttcc tccagatcca tcaccccgtt
     3541 gtcgcgcacc cacgtggcga gaaactccgt cgggtaggtt ccggtggtca ggaacttggt
     3601 gtgcgccccg ccgtcggaca gcccgggcac ggcatacgga cagttggcga tgtcgcgcat
     3661 gacctgggga tcgatggtcc tcggctcgct ctgccagcca gccttgaggt cgcaggcgat
     3721 ggatacgtcc aggaacgcgt cgatgggatg aacgcccatc cccgccgcta tctcggcaac
     3781 ggtatggccc tcccagctcc gcaggtgctc gccttcttcg gtgacgtagg cgagtaccag
     3841 gtcctgaaac ttgacgccga tccgctcctc tcgctgctcg gttccaccgc ccgcgatggg
     3901 cgcctttccc gccgcccatt cctcatggag cgcagcacga cgttgggcgt ccatcatctt
     3961 cgcctttctt tcaggcacgg tgccgatggt gagctcccgc cacagcgggg acgagtcgaa
     4021 caggttccag tcctccatgg tgaactggta gtcgatggcg caggtcaccg catgagcgat
     4081 gatgcggttt ccctcggcat tggcccgggc gagccacgca attacgtcct tgtaagactc
     4141 cgcgggcgag ccgtgctgat cgctgcgagc ggcgagcacg ttgtagatca ccggacgccc
     4201 ggacgccgcc gcaagcacgt ccgccatctt cggcggaccg atcatctgaa tgccgccgcg
     4261 gcccagacga cccagcgccg cggcgaagaa ctcgatgtcc tcgcggctca tgaggtcggt
     4321 gatcatgggc gtgccgtcgt gatcgcgctg gacggtgtcg gcgccgatga actgagccga
     4381 ccagccacag gcgccggcgt ccatggcttc ggcaagcagg cgctccgcct cggccttttc
     4441 tgcccgggtc agcggccggc ctttcgcctc ttccaccgag cccgtgagcc acaccatcag
     4501 cgggttgagc cccacgtagg agagcacgtt cacccctttc ggggtgcgat cgaggctgtc
     4561 gagaaattcc ggataggtgg tccagtccca aggcatgccc tgctgcatgc actccagcgg
     4621 cacggcttcg ttacgcgcca tggtgagcat ggccctgtcg cgatgctcgg gccgaaccgg
     4681 cgcgaagccg aacccgcagt tacccaccac cacgctggtg actccgtgct gcccggaaat
     4741 ccggcaccag ggatcccaga agatctggct gtcgtaatgg gtatgaagat cgacgacgcc
     4801 gggcgccacg atgagcccgt cggcatcgag ctcgcgatct ccgctcccgg gcgctatccg
     4861 cccgatgacg gcgaccttcc catcgcggat gccgacgtcg gcgcgatagc tgggagtgcg
     4921 gcgcccatcg atgagcatac cgttacggat gacgagatcg agatgcattg gttgatccct
     4981 gcagcggccg aatctgaaca aattatcaaa tctgttcact tgccgtcaat gcggcgggcc
     5041 gggtttcttg gtctggaatt accacgcgtt accgccattg tgaggttccg acccacgttt
     5101 tatgctcgtt gccctcatcc cgcgaaaaaa acgagaacac gatgcaggaa tttcacgtca
     5161 atgtggccac tccggatggc gagatggagt gcttcgccgc gcaccccgat ggcgaaggcc
     5221 cgtttgccgc cgtcatcctt tacatggacg cgccgggtat tcgcgaagag ctgcgcgact
     5281 tctgccggcg catcgcgggc caaggctact tctgtctgct tcccgatctc tactatcggg
     5341 agggccgcgt ccgcttcgac ctgcgcaagg gcgaggaaga gctcaagcgc atgtttgccg
     5401 tcggctcgcg gctcaccgtg gaggcggtca tgcgtgatac cggcgggatg ctcgcctacc
     5461 tggaagacaa ccctctggcg cgatcgccga cgggctgcat cggctactgc atgagcggcc
     5521 agttcgtcgt ggcggcggcg ggcacctttc ccggagagat cgccgctgcc gcgtcgcttt
     5581 acggtacccg catcgtcacc gacaagcccg actcacccca caagctcgcc gagcgcatga
     5641 agggtgagct gtacctcggc ttcgccgagc acgaccactg ggtcgaggac ttcgtcattc
     5701 ccaagctcat cgaggagctc gaccagcatg gggtgaagta cgagctcgaa gtgcatcccg
     5761 gcacggagca cggcttctgc ttcccggagc gcccatccta cgtggaggcc gcggcggaga
     5821 aagtctggga taccgtcttc gagctgttcg accgccggct gaatgccaga tgaggcgacg
     5881 ggtgcggaac gctgcatgaa gggtcttacc gagcgccagc tcaaacggcg tgagcggatc
     5941 ctggccgctg cccgcaagct catcaccgat cggggctacg acggactgac catgcgcgat
     6001 ctggccgctg agagcggggt cgcgccgaaa acgctctacc accagttcca cagcaaagag
     6061 aacctgctgc ggttggcggt ggaggagcgc taccggtatc tgtatcagat catcgacgac
     6121 gagcagatcg agcacggcat cgaccggctg ttcttcatca tcgacacggt ggcgagcacc
     6181 accgagaaca acctcgccta cgcgaaggcg ctcaggccac tgcttacgtc cgagacctcc
     6241 agcacgtttg cggccatccg catgaacacg taccgcaaag ccatcgacca gatcgaagcc
     6301 gagggggaag tcgtcgactg gatcgacgtc gacatcctcg cggcggtggt ctaccggcag
     6361 gtgaacccca tttacctcgc cccatggtac aaggagacac cctgggccac cgtcgccaga
     6421 atcgccaagc tggacatctg cctgatcctg gccgcggtga ccaccggcta cacgcagacg
     6481 acggccgaaa agaccattcg cgatctgcag aagcagctca aacgatccgg ttcccgctac
     6541 atctgagccc cctcagtcct tctccgggag cgcgtaggcg atgagatagt cggcgggcgg
     6601 cgacggcgag gtgaagtgcc cgcccgccgc gatgaccacg tactgtcggc ccccggagac
     6661 gtacgtcatg ggaacggcgt tgcccgtcgt cggcatctca tcggcccaaa gctcctcgcc
     6721 ggtatcgaga tcgaaagcac ggacgtgccg gtcggccgac gcggcgatga agatcagccc
     6781 ggccgaggtg atcgtcgggc cgcccatctg caccccgccc ttcatcatgg tgaaaggcca
     6841 gaacgcctgg tacttcaggt tgcccagcgg cacctgccac tcgatgtcgc ccgtggacag
     6901 gttcacggct gacaaggtcc cccacggcgg cttggtgcac ggcgcgccaa agcgtgacag
     6961 aaaaggccgc agcgtcgcgc agtagggcga gccctcctga ggaaagttct ccgtgccgtc
     7021 gcattgatcc cgcgggatca gattgagaat catggccaca tggtgggtat tggccaccat
     7081 gatctgacgc tccggatcca ccgccggcgc gccccagttc tgaccgccga tcaccgacgg
     7141 gtacagcggg gtttccttgt tcacggagat cggcgtgtag atcggcccgg tggtcatggc
     7201 ctcgagcgca tccc
//
LOCUS       DBNHLM010000064        12620 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_607456, whole genome shotgun sequence.
ACCESSION   DBNHLM010000064 DBNHLM010000000
VERSION     DBNHLM010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12620)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12620)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12620
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_607456"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            388..672
                     /locus_tag="AC16QF_03085"
     CDS             388..672
                     /locus_tag="AC16QF_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447567.1"
                     /translation="MKSQIDQFPKYFPGNSRSLLSNFDQHDIDSDSVRTGLRFLPSDR
                     LEINLTANYSTVDQAGPTRKTAGLYYTPDAALLPNYENAIHDNLVEDPGF"
     gene            682..2577
                     /locus_tag="AC16QF_03090"
     CDS             682..2577
                     /locus_tag="AC16QF_03090"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6447568.1"
                     /translation="MWGVTGRIDWDFNDTMTFTSLTSFREVDSELNWVIATEDVARLR
                     LSTGTVPLAVYGSNDYVDDSETFTQEFRLTSSGSGPFQWVGGLYYLNEETYRDETVPA
                     GLLVPDGDGGALVLVPAADAGDKQDNTTDSYAIFGQVTWDITDKLAITAGGRQDWEEK
                     KISRLRTPNGLAPARIFDFSTSEDWSAFTAKGGIEFQATDDLFFYATVCKGFKSGGYQ
                     GSAATELAAITPFEPEGSPIGTPGQWVIGMGYDDSLIEEQRHPERSDLDAISSEHPIA
                     LIHVSGHLTTVNSMALDVSGITAETPDPPGGAIRRQPGSNEPNGVMEETATAPLRHIY
                     SGGYGGGGGAPGADLRKALAMYAGNGVTTVQDGGASWEIHEMVREASIAGDVNLDVVL
                     YPVGMDPTFSVPEGVTLGEYQDRVKAGGIKLLLDGSPQGKTAYLSKPYEVAPHGQPDD
                     YRGYPMIPQAMADERVAHFIGRGIPMLVHCNGDAAAEMLLDALDKANETMDLGDHRTV
                     MIHAQTVREDQLDRMAALGVVPPDMMRLLWATVNRITRSGQTLGEAQRISALEAIRAV
                     TVHAAYQNFEEDLKGSLTPGKQADLVILSANPFTVDAQQILDIEVVETISRGVTVYQE
                     PSLSASL"
     gene            2626..3129
                     /gene="greB"
                     /locus_tag="AC16QF_03095"
     CDS             2626..3129
                     /gene="greB"
                     /locus_tag="AC16QF_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HIR6447569.1"
                     /translation="MSRYRPPRPRSSPYITPAGQERLAAELKELWKVKRPEVTQKVAE
                     AAALGDRSENAEYIYGKRQLREIDARIRFLAKRLDELTVVDRPPRDQGKVFFGAWVRL
                     ADETGEEARYRLVGSDEFDRAPEFISIDSPVARLLLGKSVGDEVDLPGAGGERTWEVV
                     AIGYEPA"
     gene            3183..3785
                     /locus_tag="AC16QF_03100"
     CDS             3183..3785
                     /locus_tag="AC16QF_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024890.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HIR6447570.1"
                     /translation="MTMKTTITLALLALTGLATGTSWSHHHDRDFPISIAEAEARAAE
                     AFGEIDANGDGKITSEEFANAERPMPKGRHHAHSGTHRAERGAHHRPGHHGDGPPPDQ
                     AALDAALFDALDADGDGKLSRGEFSHEKMHENHQRLRREETFARLDSNDDGVLTNDEL
                     LDRRLERLKKVDADGDGNVTRDEMKAAMREHHGGDRGPGG"
     gene            3829..4338
                     /locus_tag="AC16QF_03105"
     CDS             3829..4338
                     /locus_tag="AC16QF_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6447571.1"
                     /translation="MVLIAKQDHEAFSHLLTRHLDSIHRYLVRLAGSTADADELAQET
                     FLRVWLNARTFEAGRVAVTTWMHRIAHNVCVDELRRRRRTTSLDEAPEPGVDERGVHQ
                     ATERLEHLQAALGSLPEAQRSAIVLSRIQGFSNQDAARIMNVSVHALESLLARARRNL
                     RLRLADLSE"
     gene            4364..4780
                     /locus_tag="AC16QF_03110"
     CDS             4364..4780
                     /locus_tag="AC16QF_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447572.1"
                     /translation="MNIDDFEQGLDRWGSDLDSWPDPERDAASMLVERSERARALMVQ
                     GQALDGWLDEGRTHRAPMQLKQKILDQLPRPDIWQRAADWFASAFWPPALTGACALLL
                     GFATGTLLPAANEEALIDDVAGLAFTETYLEFEDAP"
     gene            4770..5258
                     /locus_tag="AC16QF_03115"
     CDS             4770..5258
                     /locus_tag="AC16QF_03115"
                     /inference="COORDINATES: protein motif:HMM:NF025173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HIR6447573.1"
                     /translation="MPRSKWLVLLLVLSLGANLAMAGFLAGRATGDGGGPRLLDPNLR
                     VTRMLLQQLPEERREALRPLVRDQVRSIRPSVGGIREAQRDIEAALVAEPFHPEKLEE
                     ALGAFRTHLSRSQETSHAAFVGLARSLSPEERRLLGKALAGSGRHRPHGHPPPGMPAV
                     RP"
     gene            5297..5887
                     /locus_tag="AC16QF_03120"
     CDS             5297..5887
                     /locus_tag="AC16QF_03120"
                     /inference="COORDINATES: protein motif:HMM:NF018996.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein Syd"
                     /protein_id="HIR6447574.1"
                     /translation="MSVQTALSQFVSHYEKSYPELTEIFDPEWRSPCETSEPYQDDDG
                     IVRVPWSPLARGLKVPEEHDFAGLENALETEIHPDIKAYYGSFWSGGLEAEAPQGHVS
                     LILLWNAEDASRLIENLIGHALAKRRARAPLSVFFACTDQDSELFLSVENTTGEVLIE
                     KPGHKPVDVVAGSLASFIDSLVPAPPELHPERSLLR"
     gene            5934..7211
                     /gene="aroA"
                     /locus_tag="AC16QF_03125"
     CDS             5934..7211
                     /gene="aroA"
                     /locus_tag="AC16QF_03125"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR6447575.1"
                     /translation="MGSLTIQPIQALEGDITLPGSKSISNRALLLAAEAQGQTRLVNL
                     LDSDDTAYMLRALGQLGISITENGGDHLVTGVAGPLVNDERHESLDLGLAGTALRPLT
                     AALTLGRGVFELDGTARMRERPVGHLVEGLRQLGASIRYTGAEGFPPIEVTGTGLLGG
                     RVTMQGNVSSQFLTSLLLAAPLAAGPVEVVVEGEQVSKPYLDITVDMMRRFGATVGHE
                     DHRLFRVEPGGYQSPGRYLIEGDASAASYFLAAGAIRGRGVRVHGIGAGSVQGDMAFV
                     EVLKAMGARVTVDAEWVRVEPGALTGVDLDLNHIPDAAMTLAVLALFAEGSTRIRNIY
                     NWRVKETDRLAAMATELKKLGAEVDECEDSLTVNPPKYMNAASIDTYDDHRMAMCFSL
                     AALGGVAVTINDPDCVSKTFPRYFAEFERLARR"
     gene            complement(7214..7798)
                     /locus_tag="AC16QF_03130"
     CDS             complement(7214..7798)
                     /locus_tag="AC16QF_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HIR6447576.1"
                     /translation="MDTNGLRRDPEGARRRILDSAAEHFSRHGPDGARVDAIAAAARV
                     NKRMLYHYFGDKQALFRAVLDDRAARPGGTGNTSGWDTTTARLLVWAAGLDPVGATPG
                     NLEQWTALARHLGDEQDRGRLRNDIRAGTAAMLYLALAALPALLPAHVRAILGSNDAA
                     MLQGDLRKLLNPPAELHGGSGRPRVRMQPQVRPR"
     gene            8029..8574
                     /locus_tag="AC16QF_03135"
     CDS             8029..8574
                     /locus_tag="AC16QF_03135"
                     /inference="COORDINATES: protein motif:HMM:NF016269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HIR6447577.1"
                     /translation="MRSFLRFLTLCAVCLAPAGAAWSASKVEIDARISAALESLYEIE
                     PAARELAAKASGVLVMPRVYKLGFGLGGEYGEGALLINNQAVQYYRVTAASFGFQLGG
                     QAKSEVLLFMSSEALDGFRNSDGWEAGVDGAITFIEFGVGKDFDTHTLQDPIIGFVYG
                     NKGLMYDLSLEGSKFWRIDKN"
     gene            complement(8584..9177)
                     /locus_tag="AC16QF_03140"
     CDS             complement(8584..9177)
                     /locus_tag="AC16QF_03140"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR6447578.1"
                     /translation="MTHNDGEGHMYYTYMDGPVGRLLLAGDGKRLKLIGFSTGARARG
                     AQQDWERYDEPFRPAKRQLDEYFDGRRRYFDIELAPDVTPFQGKVLEALLEIPYGETR
                     SYGEIARAIGQPRAVRAVGGANGSNPIPIVIPCHRVIGSNGSLTGFGGGLDTKQYLLD
                     LERSHSGLFEGLVGDSQQAPQRPVRAGKGQVVFTVTR"
     gene            complement(9174..10598)
                     /locus_tag="AC16QF_03145"
     CDS             complement(9174..10598)
                     /locus_tag="AC16QF_03145"
                     /inference="COORDINATES: protein motif:HMM:NF017809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HIR6447579.1"
                     /translation="MLPAPEICERARLARDPRFDGRFLTGVVTTGIYCRPICPARPAG
                     RDNVRYFPTAAAAEAQGYRACRRCRPEVAPGVPEWAISSDRVIRGLRLIDGGFLADQS
                     TAALAARLGVSTRHLNRLFHQELGATPSSLARSRRLHMALRLIVETDLGFCDVALQAG
                     YGSVRRFNAEVSGTFGRSPRALRGVRDEELTRELTLKLPVREPFDAPWMFRFLEQRAL
                     PGLEEVHGLEYRRRVGSGESWITVSQRGGALMLKVPAGTSASLSSILRRVRRLFDLQA
                     DSAVIDAHLARDPGLAPLVAASPGLRVPGAWDGFETAVRAILGQQVSVERARRLAETL
                     IHRCGEGEFPKPRALARFVPAAIGIPGKRWLAIAELSRAVADGVIDLDEGAARERLTD
                     RLLALPGVGPWTAGYIGMRVAKDPDAFVDGDWAVLKALQATPGAARRLAEPWRPWRAY
                     AVMHLWSAPVPSVDAVRLQSVSGT"
     gene            10668..11885
                     /locus_tag="AC16QF_03150"
     CDS             10668..11885
                     /locus_tag="AC16QF_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447580.1"
                     /translation="MSKAPEQPAIDYPDSDAGLDTALAAWLQDPGGADAYRQDISFRP
                     PGGAHAAPGAVEQIAAQYRFARGEHGVSVTASLDGRSLSLDAPVRLEPVSIPKPWGRE
                     LWYTGMEVRGESRVVAGEASLPLSRYLALAPARLCRRRPIVLLKILDPAATPVAGDLY
                     FEVHEHKREVYVVTHVDPTAWPDGTGAIRLGMSQSRRAAFAEDDAFRNAYLAAVKAYE
                     TVRRRIDGGERLPPETETQLREAMDGFTAFAPLRVGDVVTVHPWQPHSLQHGVRVVEF
                     QTPTYERYILSFGQRVLTQNHWDTEAVLDRIDLGPPRPESLGPVAAGIGQIADFDDFS
                     AFRVEIPPGVSRPLEIELPYAVCMGVEGRVSVNGLALDAEEACFVPASALPVTLANAA
                     ATPAVALVAAAKL"
     gene            11889..>12620
                     /locus_tag="AC16QF_03155"
     CDS             11889..>12620
                     /locus_tag="AC16QF_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6447581.1"
                     /translation="MEATRKIRVRLWRRIAPIVVVSAFYYGVLVYPVLRILGLAWPDW
                     QPGTWQLLAIMVGPLAGRLAYEAIPCSATRWLSALALTWLGICFVAFTLVLGWEVANL
                     LLPLPGRESGWALLGLTGFACLVGYVNAQRVAVRNIDVPAPQIGKRLRVAQISDVHVG
                     SRSGRFLRRVVKRTNAQDPDLVFITGDLVDFRDISQQELGSLSDLEAPAYFVIGNHER
                     YVDCEAICDRLRNLGVHVIRNDSVTI"
BASE COUNT         2231 a         4296 c         4058 g         2035 t
ORIGIN      
        1 aggcgccttc gccccgcacc accagacgcg ggtcgtcctt gaacgtcttg ttgtccgtga
       61 acggcagcca gtggttctcg agatcggctc tcgacaacgc cgaacctggc cgagcaccgg
      121 ccccggactg attcagtaat tcgctcactt atcgcttccc ccggaaactc accagccagg
      181 cccatcgaac cgcgaaatat aatgaccctt gcgctggtct gtacagcgga acctgccgct
      241 tcaaggaccg caacagggca actggacgaa cggggtcaag cccggcagag tgcgccgcat
      301 cgacaggagc cggaggaccg gctcgctaaa aacaacgttc aatgttcgat agcagcaacc
      361 ttctccagcc gcaaacgtga cggctacatg aagtcgcaga tcgaccagtt cccgaagtac
      421 tttccaggca acagccgatc gctgctgagc aatttcgatc agcacgacat tgactccgac
      481 agcgtccgca ccggtctgcg ctttctccct agcgaccgcc tggagatcaa cctgaccgcc
      541 aactacagca cggtggacca ggccggcccg acccgcaaga ccgccgggct ttactacact
      601 cccgacgctg cgctgctgcc gaactacgag aacgcgatcc acgacaacct ggtggaggac
      661 cccggcttct gaaagagcga catctggggc gtcacggggc gcatcgactg ggatttcaac
      721 gacaccatga cgttcacctc gctgacctcc ttccgcgagg tggattcgga gctgaattgg
      781 gtcatcgcca ccgaggacgt cgcccggctg cgactctcca ccgggacggt gccgcttgcc
      841 gtctacggca gcaacgacta cgttgacgac tcagaaacct tcacgcagga gttcaggctc
      901 acctcgtccg gcagcgggcc tttccaatgg gtgggcgggc tctactacct gaacgaagaa
      961 acctaccgcg acgagaccgt acccgccggc ctgctggtgc ccgacggcga tgggggcgcc
     1021 ctcgttctcg tgccggccgc ggatgcgggc gacaagcagg acaacaccac cgacagctac
     1081 gccatattcg gtcaagtcac ctgggacatc acggacaagc tggcgatcac cgccggtggc
     1141 cgtcaagact gggaagaaaa gaagatcagc cggttgcgca cgccgaacgg cctggcgccg
     1201 gcgcggatct tcgatttcag caccagcgag gactggagcg ccttcacggc taagggcggc
     1261 atcgagtttc aggcgaccga cgacctgttc ttctacgcaa ccgtctgcaa agggttcaag
     1321 tcaggcggct accagggtag cgcggccacg gagctcgccg ccattacgcc cttcgagccc
     1381 gagggctcgc ctatcggcac gccgggtcag tgggtgatcg gcatgggcta cgacgattcg
     1441 ctcatcgagg agcagcgcca tccggagcgt tccgacctgg acgcgatctc aagcgagcat
     1501 cccatcgcgc tcatccacgt ttccggtcat ctgaccaccg tgaactccat ggcgctggac
     1561 gtaagcggca tcacggccga gacgcccgat cccccgggcg gcgcgatccg ccgccagccg
     1621 ggcagcaacg agcccaacgg cgttatggag gagacggcta ccgcgccgtt gcgccacatc
     1681 tattctggcg gctatggcgg cggcgggggg gcgcccggtg cggacctgcg caaggccctt
     1741 gcgatgtacg ccgggaacgg ggtcaccacg gtccaggacg ggggcgcttc ctgggagatc
     1801 cacgagatgg tccgggaagc atccatcgcc ggggacgtca atctcgacgt ggtgctgtac
     1861 cccgtgggta tggacccgac gttttccgtt ccggaaggcg tcacgctggg cgagtaccag
     1921 gaccgggtga aggccggtgg catcaagctg cttctggatg gttcgcccca gggcaagacg
     1981 gcctacctga gcaaacccta cgaggtggcg ccccacggcc agccggacga ttaccgcggc
     2041 tatcccatga ttccgcaggc catggcagac gagcgggtgg cccacttcat cggccgcggc
     2101 atccccatgc tggtgcactg caacggcgac gctgccgccg agatgctgct cgacgccctg
     2161 gacaaggcga atgagaccat ggatctgggc gatcaccgaa cggtgatgat ccacgcccag
     2221 acggttcggg aagatcagct cgaccgcatg gcggcactcg gcgtggtgcc gcccgacatg
     2281 atgcgtctgc tgtgggccac ggtgaatcgc atcacccgca gcggccagac cctgggcgag
     2341 gctcagcgca tcagcgccct ggaggcgatc cgcgcggtga ccgtccacgc cgcctaccag
     2401 aatttcgagg aagacctcaa agggtccctg acaccgggca agcaggcgga tctggtgatc
     2461 ctctcggcca acccgttcac cgtggacgcc cagcagattc tggacatcga ggtggtggaa
     2521 acgatctccc ggggtgttac cgtctaccag gaaccctcgc tctccgcgag cctttaggcc
     2581 ggacccaatg tctgctggca aaaaaacctt aacgcacggg cgccgatgag ccgctaccgc
     2641 ccgccccggc ccagatcctc cccctacatc acgccggccg gccaggagcg tctggctgcc
     2701 gaactcaagg agctgtggaa ggtcaaacgc cccgaggtga cccaaaaggt cgctgaagca
     2761 gccgcgctgg gcgaccgttc cgagaatgcc gagtacatct acggcaagcg gcagctcaga
     2821 gagatcgacg cccgcatccg gtttctcgcc aagcggctgg acgagctgac ggtggtggac
     2881 cgccccccga gggaccaggg caaggtcttt ttcggcgcgt gggtgaggct tgcggacgag
     2941 accggcgaag aggcccgcta ccggctggtg ggctcggacg agttcgaccg ggcgcctgag
     3001 ttcatcagca tcgactcccc cgtcgcccgg ttgctgctgg gcaagtcggt gggggacgag
     3061 gtcgacctgc ccggagccgg cggcgagaga acgtgggagg tcgtggccat cggctacgag
     3121 ccagcctgac gagcgacggg tttttcgact ccgtgcgtat aaccgccagg aaaccaacag
     3181 gaatgaccat gaagacgacc atcacccttg ctctgctcgc cctgaccggt ctcgccaccg
     3241 ggacgagctg gagccatcac cacgaccggg acttcccgat atccatcgcg gaagcagagg
     3301 cccgcgcggc cgaggcgttc ggagaaatcg acgcgaacgg cgacggaaag atcaccagcg
     3361 aggaattcgc caacgcggag cggccgatgc cgaaagggcg ccatcacgct cactccggta
     3421 cccatcgcgc cgaacgggga gcgcatcatc gaccgggcca tcacggcgat gggccgccac
     3481 ccgaccaggc cgccctggac gccgcgttgt tcgacgccct cgacgccgat ggcgacggca
     3541 agctgtcccg gggcgagttc agccacgaga agatgcacga gaaccatcag cgtctgcgca
     3601 gggaagagac gttcgcgcgt ctggacagca acgacgacgg cgtgctcacg aacgatgagt
     3661 tgctggaccg tcgtctcgag cgtttgaaga aagtggacgc cgacggtgat gggaacgtca
     3721 cccgcgacga gatgaaagcc gcgatgcgcg agcatcacgg cggcgaccgc gggcccggcg
     3781 gctgacccga cacgctcgag ggtgggagag ccgacggacg aagcgctgat ggtgctgata
     3841 gccaagcagg atcacgaggc gttcagccat ctgctgactc gtcatctcga ttccattcac
     3901 cgctacctcg tccgtctcgc cggctcgacc gccgacgccg acgaactcgc ccaggagacg
     3961 ttcctgcgcg tatggctcaa tgcccgcacc ttcgaggccg gccgggtggc ggtaaccacc
     4021 tggatgcacc gcatcgccca caacgtctgc gtcgacgagt tgcgacgccg ccgccggacg
     4081 acgagcctag acgaagcgcc cgaacccggg gtcgacgagc gtggcgtgca ccaggccacc
     4141 gagcggctcg agcatctgca ggccgcgctc ggctcgctgc ccgaggcgca gcgctcggcc
     4201 atcgtgctga gccggattca gggtttttcg aaccaggacg cggcccggat aatgaatgtc
     4261 agcgtccacg ccctggagtc gctgctggcg cgagcccggc gcaacctcag attacggttg
     4321 gccgacctca gcgaatgagc gggttaacaa ggggagccac gcgttgaaca tcgacgactt
     4381 cgaacagggc ctggaccgct ggggatccga tctcgactcc tggccggatc cggaacgcga
     4441 cgccgcgtcg atgctggtgg aacgttcgga gcgggcccgg gcgctcatgg ttcaggggca
     4501 ggctctggat ggctggctcg acgagggccg aactcatcgc gcgccgatgc agctcaaaca
     4561 gaagattctc gaccagctgc cgcgcccgga catctggcag cgcgcggccg actggttcgc
     4621 ctccgcgttc tggccaccgg cgctgaccgg cgcttgcgcg ctgctgctgg gcttcgccac
     4681 gggcacgctg ctgccggcgg caaacgaaga agccctgatc gatgacgtcg cagggcttgc
     4741 gtttaccgag acctatctgg agttcgaaga tgccccgtag caaatggctc gttcttctgc
     4801 tggtcctgtc gctgggcgca aacctcgcca tggccggatt cctggcggga cgcgcaaccg
     4861 gtgatggggg cggacctcgt ctgctcgatc ccaatctgag ggtgacccgg atgcttctgc
     4921 aacagcttcc ggaggagcgc cgcgaggcgc tgcgcccgct cgtgcgcgac caggtcaggt
     4981 cgatccggcc aagcgtcggc gggatccgtg aggctcagcg ggatatcgaa gccgcgctgg
     5041 tggccgagcc gttccacccc gaaaagctcg aggaggcgct tggggcgttc cgtacccatc
     5101 tgagccgcag ccaggaaacc agtcacgccg ccttcgtcgg gcttgcgcga tctctctcac
     5161 ccgaggagcg tcgcctgctc gggaaggcgc tggcgggctc aggccgccat cggcctcacg
     5221 ggcacccgcc ccccggcatg cctgccgtgc gcccgtagca ggtaaaatac cgcctcgatt
     5281 ccaagcgaga tcttcgttgt ccgtccagac cgcgctttcc caattcgtct cccactacga
     5341 gaagtcctac cccgagctta cggagatatt cgatcccgaa tggcgctcgc cctgcgagac
     5401 gagcgagccc taccaggacg acgacggcat cgtccgggta ccctggagcc cgcttgcccg
     5461 cgggctgaaa gttcccgagg agcacgactt cgccggcctg gagaacgccc tggagactga
     5521 aatacacccg gacatcaagg cttactacgg cagcttctgg tcgggagggc tcgaggcaga
     5581 ggcgccccag ggtcacgtaa gcctcattct tctgtggaac gcagaggatg cttcccggct
     5641 gatcgagaac ctcatcgggc acgcgctggc caaacggcga gccagggcgc ctctcagcgt
     5701 cttcttcgcc tgcaccgatc aggactcgga gctctttctc tccgtggaga acaccaccgg
     5761 tgaggtgctg atcgagaagc ccggtcacaa gccggtggac gtggtggccg gaagcctcgc
     5821 cagcttcatc gactcgctgg tgcccgcacc tccggagctg caccctgagc gcagtctgct
     5881 gcggtaacgc gcaggttcgc gcccgcaccc ggtttgtcgt ttaatcccac gccatgggat
     5941 ctctgaccat tcaacccatc caggctctgg aaggcgacat cacgctgccg ggctccaaga
     6001 gcatttcgaa ccgcgccctg ctcctggccg ctgaagccca aggccagacc cgcctggtga
     6061 acctgctcga cagcgacgac acggcctaca tgcttcgcgc cctggggcag ctgggaatat
     6121 ccataaccga gaacggcggc gaccatctcg tgaccggcgt ggccgggccc ctggtcaacg
     6181 acgagcgcca cgagtctctg gatctcggcc tggccggcac cgctctccgg ccactcaccg
     6241 cagcactgac cctgggccga ggcgtattcg agctggacgg caccgcgaga atgcgcgaac
     6301 ggcccgtcgg gcacctggtg gaagggctgc gccagctcgg cgcgagcatc cgctacaccg
     6361 gcgcggaggg cttcccgccc atcgaggtga ccggcaccgg ccttctcggc gggcgcgtga
     6421 ccatgcaggg caacgtgtcc agccagttcc tgacctccct gctgctcgcc gcccccctgg
     6481 ccgcgggacc ggtggaggtc gtcgtggagg gcgagcaggt gtccaagccc tatctcgaca
     6541 tcaccgtaga catgatgcgc cgcttcggcg cgaccgtcgg ccacgaggat catcgcctgt
     6601 ttcgcgtcga gcccggcggc taccaatcgc ccgggcgcta cctcatcgaa ggcgacgcat
     6661 ccgccgcttc ctatttcctc gccgcgggcg ccatccgtgg ccggggcgtc cgcgtgcacg
     6721 gcatcggagc aggttccgtc cagggcgaca tggccttcgt cgaggtccta aaggccatgg
     6781 gggcgcgggt gaccgtagac gccgaatggg tgcgggtcga gccgggagca ctcacgggcg
     6841 tcgatctgga cctgaaccac atccccgatg ccgccatgac gctggccgtg ctcgcgctct
     6901 tcgccgaggg ttccacacga atccggaaca tatacaactg gcgggtcaag gagacggacc
     6961 gccttgccgc catggcgacg gagcttaaga agctgggtgc cgaggtagat gaatgtgagg
     7021 actcacttac agtcaatcct ccaaaataca tgaacgcagc ctctatcgac acctacgacg
     7081 accaccgcat ggccatgtgt ttctcgctgg ccgcgttggg cggcgtggcg gtcacgatca
     7141 acgacccgga ctgcgtcagc aagacgtttc cgcgctactt cgcggagttc gagcgtctcg
     7201 cacgccgctg acctcagcgc ggacgaacct ggggctgcat gcgtaccctg ggacgcccgc
     7261 tgccgccgtg gagctcggcg ggcggattca gaagctttct aaggtccccc tgcagcatcg
     7321 cggcatcgtt ggagcctaag atggcccgca cgtgagccgg caacagcgcg ggcaaggcgg
     7381 ccaaggcgag ataaagcatc gccgcggtac cggcccggat atcgttgcgc aaccggcccc
     7441 tgtcctgctc atcacccagg tgcctggcca gcgccgtcca ctgctccagg tttcccggcg
     7501 tcgccccgac cggatccaga ccggctgccc agaccagcag gcgcgccgtc gtggtatccc
     7561 agccggacgt gttccctgtg ccgccaggcc gcgccgcgcg atcgtccagg accgcccgga
     7621 aaagcgcctg cttgtcacca aagtagtgat agagcatccg cttgttgaca cgggcggcgg
     7681 ccgcgatggc gtccacccgg gcaccgtccg ggccatggcg cgagaagtgc tcggcagccg
     7741 aatccagaat tcgccggcga gctccctcgg ggtcacgcct gaggccgttt gtatccattt
     7801 ggttactcaa tactgccagt cagcggctga aaagatcata tgtatccatt tagttactac
     7861 tttcgcctgg gctccgtcag agaatgtccg gccggtatct ggaccattcg cttgccggcc
     7921 ctccgcgaca ggtatagtaa acaattggtt acataatttc tgccctcgac caaggctccg
     7981 cccggtggta taaaacgccc cggtctcaac cgagcaggat gatgcccgat gcgttcgttc
     8041 cttcgattcc tcaccctttg cgccgtctgc cttgcacctg ccggggccgc atggtctgcc
     8101 tccaaggtcg agatcgacgc ccgcatctcc gccgcccttg aatcgctcta cgagatcgag
     8161 cccgccgcgc gggaactggc ggcaaaagcc tccggcgtgc tggtgatgcc gcgcgtctac
     8221 aagctcgggt tcgggctggg cggcgagtac ggcgagggcg cgctgctgat caacaatcag
     8281 gcggtgcagt actaccgggt gacggccgcg tcgttcggtt tccagctcgg cggccaggcc
     8341 aaatccgagg tcttgctgtt catgtcatcc gaagcgctgg acggcttccg caacagtgac
     8401 ggctgggagg ccggcgtgga cggggccatc accttcatcg agttcggcgt cggcaaggat
     8461 ttcgacaccc acaccctgca ggacccgatc atcggcttcg tctacggcaa caagggcctg
     8521 atgtacgatc tttcgctgga aggctccaag ttctggagga tcgacaagaa ctgagcgggc
     8581 cgtctagcgg gtgaccgtga acaccacctg ccctttgccc gcacgcacgg gtcgttgggg
     8641 cgcctgctgg ctgtcaccga cgagaccctc gaagagtccg gagtggctgc gctccaggtc
     8701 caggaggtac tgcttggtgt ccaggccacc gccaaagccc gtgagcgagc cgttgctgcc
     8761 gatgaccctg tggcagggga tcacgatagg gatcggattg ctgccgttgg ccccgccgac
     8821 ggcccgcacc gcgcgaggct gaccgatggc ccgcgcaatc tcgccgtaag aacgtgtctc
     8881 cccatacggg atctcgagca gcgcctccag caccttgccc tgaaacgggg tcacgtcggg
     8941 cgcaagctcg atgtcgaagt agcggcgccg gccgtcgaag tactcgtcga gctggcgttt
     9001 cgccgggcga aacggctcgt cgtagcgttc ccagtcctgc tgcgcaccgc gcgcccgggc
     9061 gcccgtggag aatccgatca gcttcaggcg cttgccgtca ccggcgagca gcagtcggcc
     9121 caccgggccg tccatatagg tgtagtacat gtgcccttcc ccgtcgttgt gggtcacgtc
     9181 cccgacaccg attgtaaccg gaccgcatcg accgacggca cgggagcaga ccagagatgc
     9241 atgaccgcgt acgcccgcca gggtcgccac ggctcggcca agcggcgcgc agctcccggt
     9301 gtggcctgca acgccttaag caccgcccag tcaccgtcaa cgaacgcatc gggatccttc
     9361 gcgacccgca tgccgatgta gccggccgtc cacggaccca ccccgggtag agccagcagt
     9421 cggtccgtca gccgttcacg agctgcaccc tcgtcgagat cgatgacacc atccgccacc
     9481 gcccgggaaa gctcggcaat ggcaagccaa cgcttgcccg gaattccaat ggcggccggc
     9541 acgaagcgcg ccagggcgcg gggcttcggg aactcccctt ccccgcagcg gtggatcagg
     9601 gtctcggcaa ggcgtctggc ccgttccacg ctgacctgtt gtcccagtat ggcgcgaacg
     9661 gcggtttcga agccgtccca ggcacccggc accctcaatc ccggcgacgc ggcaacgagg
     9721 ggcgcaaggc ccggatcccg cgcgaggtgc gcgtcgatga cggcagagtc cgcctggagg
     9781 tcgaacagcc gcctgacgcg ccgaagtatc gaggacagcg acgccgaagt gcccgcgggc
     9841 actttcagca tcaacgcgcc cccgcgctgg gataccgtga tccagctttc cccggatccg
     9901 acccgccggc gatactcgag cccgtgcacc tcctcgagac cgggcaacgc gcgttgctcg
     9961 agaaagcgga acatccacgg cgcatcgaaa ggctcacgga ccggcagctt aagggtcagc
    10021 tcccgggtca gttcttcgtc acgcaccccg cgcagagccc gaggcgaacg tccgaacgtc
    10081 ccgctcacct cggcgttgaa gcgccggacg ctgccgtagc ccgcctgcag ggcaacatcg
    10141 cagaagccca ggtcggtctc gacgatgagc cggagagcca tgtgcagccg ccgggacctc
    10201 gccaggctgc tgggcgtggc cccgagctcc tggtgaaaga gcctgttgag gtgccgggtg
    10261 ctcacgccga gcctggctgc gagcgcggcc gtgctctgat cggccaggaa tcccccgtca
    10321 atcagccgca gccccctgat caccctgtcg ctggagatcg cccactccgg cacccccggc
    10381 gcgacctccg gccgacagcg gcgacatgcc cgataccctt gggcttccgc cgccgcagcc
    10441 gtaggaaagt agcgcacgtt gtcccggcca gcgggccggg ccgggcagat gggcctgcaa
    10501 tagatcccgg tggtgacgac gccggtgagg aaccggccgt cgaagcgggg gtctctggcc
    10561 aggcgggcgc gctcgcagat ctctggggca ggtaacatgc tggcctcttc agcagcttcc
    10621 ccgactataa tcccgacctc gcgaaaaaac tcgccggaat cggacatttg tccaaagcgc
    10681 ccgaacagcc cgccattgac tatcccgaca gcgacgccgg gctggacacg gccctggccg
    10741 cttggctgca ggatcccggt ggggcggacg cctatcgtca ggacatttcc ttccgaccgc
    10801 ctgggggcgc tcacgctgct ccgggcgctg tcgagcagat tgccgcgcaa taccgattcg
    10861 cccgaggcga gcacggcgtg agcgtgaccg cgtcgctgga tggacgaagc ctgtccctcg
    10921 atgcccccgt ccgcctggag ccggtcagca taccgaaacc ctggggccgg gaactgtggt
    10981 acacgggtat ggaggtacgc ggcgaaagtc gcgtggttgc cggcgaggcc agcctgcccc
    11041 tgtcgcgcta tcttgcgctc gcaccggcgc gcctgtgccg gcgacgtccg atcgtccttt
    11101 tgaaaattct ggatcctgcc gccacgcccg tggcgggcga cctctatttc gaggtgcacg
    11161 agcacaagcg cgaggtgtac gtcgtcaccc acgtggaccc gaccgcatgg cctgacggca
    11221 cgggggcgat ccggctcggg atgagccagt cgcgacgcgc agcttttgcc gaggacgacg
    11281 cgttccgaaa cgcctacctc gcggcggtga aggcctacga gaccgttcgc cgccgcattg
    11341 acgggggcga acggttgccg ccggaaaccg agacgcagct gcgcgaagcc atggacggct
    11401 tcacggcctt tgcgccgctg cgggtcggtg acgtggtgac cgtccacccc tggcagccgc
    11461 attcgctgca gcatggtgtg cgcgtcgtcg agttccagac cccaacctac gagcgctaca
    11521 ttctgtcgtt cggccaacgg gtgctcaccc agaaccactg ggacaccgag gccgtcctcg
    11581 accggatcga tcttggccct ccccggccgg agagtttggg gccggtcgcc gccggcatcg
    11641 ggcagatcgc ggacttcgac gatttctccg cctttcgggt ggagatccct cccggggtct
    11701 cccgcccgct ggaaatcgag ctgccttacg ccgtgtgcat gggggtagaa ggccgcgtat
    11761 cggtgaacgg gctcgccctg gatgcagaag aggcctgctt cgtgcccgcc tcggcactcc
    11821 ccgtgaccct ggcaaacgct gccgccaccc cggccgttgc tctggtggcg gcggccaagc
    11881 tctgagccat ggaggcgacg cgcaagatcc gggtcaggct gtggcggcgc atcgcaccca
    11941 tcgtcgtcgt ttcggccttc tactacggcg tcctggtcta tcccgttctg cgcattctcg
    12001 gcctggcatg gcccgactgg cagccgggca catggcagct gctcgcaatc atggtgggtc
    12061 ccctcgcggg acgcctggcc tacgaggcca taccctgcag cgcgacccgc tggctgtcgg
    12121 cgctggcgct gacctggctg gggatctgct tcgtcgcgtt caccctggtg ctcggctggg
    12181 aggtggccaa cctgctgctg ccgctgccgg gtcgcgaatc cggctgggcg cttctgggac
    12241 tgaccgggtt tgcctgcctg gtgggctacg tcaacgcgca gcgcgtggcg gtcaggaaca
    12301 tcgacgttcc cgcaccacag atcggcaagc gcctgcgcgt agcgcaaatc agcgacgtgc
    12361 acgtcggctc ccgcagcggc cggtttctcc gccgggtggt gaaacgcacg aacgcccagg
    12421 atccggacct ggttttcatc accggcgact tggtggactt cagggacatc tcccagcagg
    12481 agctgggctc cctgagcgac ctcgaagcgc ccgcctactt cgtcatcggc aaccatgagc
    12541 gctacgtcga ctgcgaggcc atctgcgatc gcctgaggaa tctcggggtg cacgtgatac
    12601 gcaacgacag cgtgacgatc
//
LOCUS       DBNHLM010000065         3394 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1565861, whole genome shotgun sequence.
ACCESSION   DBNHLM010000065 DBNHLM010000000
VERSION     DBNHLM010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1565861"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="AC16QF_03160"
     CDS             <1..401
                     /locus_tag="AC16QF_03160"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR6447582.1"
                     /translation="TCISSFRQLFALDFSYYNYHYDLQDTARYYALFRQLMGHWQACG
                     HGRIHEVSYEALTADPESEVRHLLSYLELPWHPACLEFHRNRGAVATASTVQVRQPLY
                     RSSVSRWKRYEAHLQDAIAVLEQNGIEWRD"
     gene            459..1436
                     /locus_tag="AC16QF_03165"
     CDS             459..1436
                     /locus_tag="AC16QF_03165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6447583.1"
                     /translation="MKFGVLQFFSWPGRRGELHQVYERGLERVRIMDANGYDAVWIAE
                     HHFSTYSVCPSIHMMGAHFAANTKRLRIGTGVSLAAFYNPLRLAEEVALLDVLSGGRV
                     NWGAGSGFDPTEFRAFGIERGDRYPQFRENVEIVLKAWRSDRLTYHGRFHCYEDIEVL
                     PKPLQDPIPVWMAASSVDSVRWAAQEGFSILMDPHSSMPDIAAKQQIYHETLIAHGHD
                     PEGRDLPVARLLAIAPTDAQAREVAEAGAQWTTGSYAKQPLALKKKDNEQDPVQRYID
                     EIILWGSPERVADRLLEQQEDGHLNYLLCAPLSNQSFRLFTDEVLPRIA"
     gene            complement(1476..2327)
                     /locus_tag="AC16QF_03170"
     CDS             complement(1476..2327)
                     /locus_tag="AC16QF_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447584.1"
                     /translation="MLKVFGFVRRHPNLTHDEYRAGHVGYHNSFGRRLNNIRGYLLNV
                     RANATIEETLGTDLVQSITLDEPPGFDDLWDGWGQLLFDSLDDYLAARSPARDMAGPD
                     GLQMDPGVAGVGGDFEYLYGGSPFQFHLAENVVVPVVRPERKLFKLVQFVKRPDGLEP
                     ELFRAYLSGRYARAAATMGGLCGLVVNFRTGVDVMTHFFAPDAEGFTPEGAAIRERFL
                     SYWDAMIEYWLESPADFVAGRATGSAAENMAALEKTLMSRSFFREVDETVAVLPNRAP
                     PPPFYHR"
     gene            complement(2338..3210)
                     /locus_tag="AC16QF_03175"
     CDS             complement(2338..3210)
                     /locus_tag="AC16QF_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447585.1"
                     /translation="MYKVFAFLKRNPNLTHDEYRAGHVGYHCGQSRRLKNIRGYLVNV
                     WDNQPLAEKIGDLAGEIAFNQPDGFLDYWDGFPQVYFDDREAWVNARTVEPNRATADG
                     LVVDPDWSLGDSPHLFMPVPGEPTQFQAHHVHMEEHVVVAVRRPEFRLTKLIQFFQKR
                     EGLTDAEFADRLLGEYAPLAGSMPGLWGYVANLRDADPEAAMRGFFAPDSWVFGEEGR
                     ALHGRFCSLWRGANELHFDSVEAFAAARRDDRVRDRLLELERELFSSMWHVEVDENLI
                     VIPNRGPAPDFYFR"
BASE COUNT          612 a         1152 c         1089 g          541 t
ORIGIN      
        1 acacctgcat cagcagcttc cggcagcttt tcgccctgga tttctcctac tacaactacc
       61 actacgatct gcaggacacg gcccgctact acgcgctgtt ccgacagctg atggggcact
      121 ggcaggcctg cggccacgga cgcatccacg aggtctccta cgaggccctg actgccgatc
      181 ccgaatccga ggtgcggcat ttgctcagct acctcgagct tccctggcac ccggcatgcc
      241 tggagtttca tcgcaatcgc ggcgcggtag ccaccgccag cacggtgcag gtgaggcagc
      301 ccctgtaccg gtcctcggta tcgcgatgga aacgctacga agcgcatctg caggacgcga
      361 ttgcggtttt agagcagaac ggcatcgagt ggcgcgactg agagatctcg gttctgcgtg
      421 ataggcttca ccgaaacatc gcaggtgtcg gcgcacgaat gaaattcgga gttctgcagt
      481 tcttcagctg gcccggccgc cgcggcgagc tgcaccaggt ctacgagcgc ggccttgagc
      541 gcgtgcgcat catggacgcc aacggctacg acgccgtgtg gatcgccgag caccacttca
      601 gcacctacag cgtctgcccg tccattcaca tgatgggcgc ccacttcgcg gccaacacca
      661 aacgcctgcg catcggcacc ggcgtttccc tggccgcctt ctacaacccg ctgcggctgg
      721 ccgaggaggt ggcgctgctg gacgtgctct ccggcggccg ggtcaactgg ggagcaggca
      781 gcggcttcga tcccacggag tttcgcgcct tcggaatcga gcggggcgac cgctaccccc
      841 agttccggga gaacgtcgag atcgttctca aggcctggcg ctcggacagg ctcacctacc
      901 acggccgctt ccactgctac gaggacatcg aggtgctgcc caagccgctc caggacccga
      961 tcccggtctg gatggcggct tcttccgtcg acagcgtacg ctgggccgcg caggaggggt
     1021 tttccattct catggatccg cactcgtcca tgcccgacat cgccgccaag cagcagatct
     1081 accacgagac gctgatcgcc cacggccacg atccggaggg ccgcgacctg ccggtggcgc
     1141 ggctgctcgc catcgcgccg acggacgccc aggcccgcga ggtggccgag gcaggcgctc
     1201 agtggaccac gggcagctac gccaagcagc cgctggccct gaagaaaaag gacaacgagc
     1261 aggacccggt gcagcgctac atcgacgaga tcatcctctg gggcagcccc gagcgggtgg
     1321 ccgatcggct gctggagcag caagaggacg ggcacctgaa ctatctgctc tgcgcgccgc
     1381 tgagcaacca gtcgttccgg ctcttcaccg acgaggtcct gccccgcatc gcctgatcgg
     1441 caggcgcgcg ctcggcaagc gcacggagcg acgaatcagc gatggtagaa gggcggcggc
     1501 ggtgcccgat tcggcagcac ggccacggtt tcgtccactt cccggaaaaa gctgcggctc
     1561 atcagcgtct tttcgagcgc ggccatgttc tccgccgcgc tgcccgttgc gcggccggca
     1621 acgaagtcgg ccgggctctc cagccagtac tcgatcatgg cgtcccagta agaaaggaaa
     1681 cgctcgcgga tggccgcgcc ctcgggggtg aacccttcgg catcgggcgc gaagaaatgg
     1741 gtcatcacgt cgacgcccgt ccggaagttc acgacgagcc cgcagaggcc gcccatggtc
     1801 gccgccgccc gcgcgtagcg ccccgaaagg taggcgcgaa acagctcggg ctccagcccg
     1861 tcggggcgct ttacgaactg cacgagcttg aacagcttgc gctcgggcct gaccaccggg
     1921 accaccacgt tctcggccag gtgaaactga aacggcgagc cgccgtagag atactcgaag
     1981 tccccgccga cgccggccac acccgggtcc atctgcaggc cgtccgggcc ggccatgtcc
     2041 cgggcgggag aacgggcggc caggtagtcg tcgagggagt cgaacagcag ctggccccag
     2101 ccgtcccaca gatcgtcgaa gccgggcggc tcgtccaggg tgatgctttg caccaggtcg
     2161 gtgccgagcg tctcctcgat ggtggcgttg gcgcgcacgt tcaggaggta gccgcggatg
     2221 ttgttgagcc ggcggccgaa ggagttgtgg taaccgacgt gtcccgcacg gtactcgtcg
     2281 tgggtgaggt tggggtggcg ccggacaaat ccaaaaacct tgagcattcg atccccctca
     2341 gcggaaataa aaatcggggg cgggcccgcg attcggtatg acgatgaggt tctcgtccac
     2401 ctccacgtgc cacattgaag agaaaagctc acgctcgagc tcgagcagac ggtctcgcac
     2461 ccgatcgtcg cggcgggcgg cggcaaaggc ttcaacggag tcgaagtgca gctcgttcgc
     2521 gccgcgccac agggaacaga aacggccgtg cagtgccctg ccctcctcgc cgaacaccca
     2581 gctgtccgga gcgaagaagc cgcgcatcgc agcctcgggg tccgcatcgc gcaggttcgc
     2641 cacgtagccc cacaagcccg gcatgctgcc ggccaggggc gcatactcgc cgagaagcct
     2701 gtcggcaaac tcggcatcgg tgagcccttc gcgtttctga aagaactgga tcagcttggt
     2761 gagccggaac tccggccgcc gcacagcaac cacgacgtgc tcctccatgt gcacgtgatg
     2821 ggcctggaac tgcgtgggct cgcccggcac gggcatgaac agatgcgggc tgtcgcccag
     2881 gctccagtcc gggtccacga ccagcccatc ggccgtggct cggttgggct ccacggtgcg
     2941 ggcgttcacc cacgcttccc ggtcgtcgaa gtagacctgg gggaacccgt cccagtaatc
     3001 gagaaaaccg tccggctggt tgaaggcgat ctccccggcc agatcaccga tcttctccgc
     3061 caggggctgg ttgtcccaca cgttcaccag gtagccgcgg atgttcttca gccgccgcga
     3121 ctgaccgcag tgatagccca cgtgcccggc gcggtactcg tcgtgagtca ggttgggatt
     3181 gcgtttcaag aacgcgaaga ctttgtacat ggatccggca gctccccggc aaccgctcgc
     3241 tcaacccgga gcatacacga agctcagccg gcttcattgc caacggggca ggagaagcgc
     3301 cggcaccgtg tcccgcgggc cggcgggacg cgctatgatt cggcctgacg ccgcaccggt
     3361 cttccaacgt ccgcggcccc accatcgcag gaac
//
LOCUS       DBNHLM010000066         2186 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_607894, whole genome shotgun sequence.
ACCESSION   DBNHLM010000066 DBNHLM010000000
VERSION     DBNHLM010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_607894"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="AC16QF_03180"
     CDS             complement(<1..520)
                     /locus_tag="AC16QF_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR6447586.1"
                     /translation="MSKAKFERTKPHVNVGTIGHVDHGKTTLTAAITKVLAEAGGADF
                     KAFDEIDKAPEEKARGITIATAHVEYQTETRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHVLLARQVNVPHIVVFLNKVDQVDDEELLELVELEVRELLSK
                     YEFPGDDIPIVKG"
     gene            complement(600..675)
                     /locus_tag="AC16QF_03185"
     tRNA            complement(600..675)
                     /locus_tag="AC16QF_03185"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(640..642),aa:Thr,seq:ggt)
     gene            complement(706..779)
                     /locus_tag="AC16QF_03190"
     tRNA            complement(706..779)
                     /locus_tag="AC16QF_03190"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(745..747),aa:Gly,seq:tcc)
     gene            complement(831..915)
                     /locus_tag="AC16QF_03195"
     tRNA            complement(831..915)
                     /locus_tag="AC16QF_03195"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(879..881),aa:Tyr,seq:gta)
     gene            1147..>2186
                     /locus_tag="AC16QF_03200"
     CDS             1147..>2186
                     /locus_tag="AC16QF_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIR6447587.1"
                     /translation="MRGQRLAIAGLAALMFLPGVAAATNGYFAHGYGTANKGLAGAGV
                     ALPQNALTIATNPAGLTRLGHRVDLGAALFSPWRDHSISGFPTDPSTLPPGSPPTFPL
                     ETGSYESGSEYFLIPHFGYNRPLDERSAIGIAAYGNGGMNTDWPGHANPIRSQGGQGT
                     DPSNQSTFNPCAQPSPFFGGTGTYCNGGAGVDLMQLFITPTYAREVSDSVSLGVTAVL
                     AYQRFEATGVASFAGVSNDPTKLSNNGHEDSYGAGIRIGLLASLSPTVDVGLAYQSKI
                     WMSEFDDYAGLFAGDGDFDVPSNFTAGIAWKTSDRSTLVFDIQHIRYSEVDAVGNPML
                     PAFMTCAMRQTPT"
BASE COUNT          392 a          795 c          611 g          388 t
ORIGIN      
        1 agcccttgac gatcggaatg tcgtcgcccg ggaactcgta cttcgagagc agctcccgaa
       61 cctcgagctc caccagctcc agcagctcct cgtcgtccac ctggtccacc ttgttcagga
      121 agaccacgat gtgcggaacg ttcacctgac gcgccagcag cacgtgctcg cgcgtctgcg
      181 gcatcggacc gtccgccgca ctcaccacca ggatcgcgcc gtccatctgc gccgcgcccg
      241 tgatcatgtt cttcacgtag tcggcgtggc ccggacagtc cacgtgcgcg tagtggcgcg
      301 tctccgtctg gtattccacg tgcgccgtcg caatcgtgat gccccgcgcc ttttcttccg
      361 gcgccttgtc aatctcatcg aacgccttga aatccgcacc gccagcttcc gccaagacct
      421 tcgtgatcgc cgccgtcagc gtcgtcttac catggtccac gtgaccaatc gtccccacgt
      481 tcacgtgcgg cttcgtacgc tcgaattttg ccttggacat tccgttctct cccggatacg
      541 cgtcttgctg tggtttgcgt caactgcccg acacggtaac gaccgcgtca cgtctcaagt
      601 ggagcccata tccggatttg aaccggagac ctcttcctta ccaaggaagt gctctaccga
      661 ctgagctata tgggccgcta accgtttcca ccccggccgt ccaactggag cgggtgatgg
      721 gaatcgaacc cacatcatca gcttggaagg ctgaggttct accattgaac tacacccgct
      781 gactttccct gcaacgggga ccggcactct ccgccgcggt cccgagtcat tggtggaggg
      841 gggaggattc gaacctccga aggctgagcc agcagattta cagtctgccc cctttgaccg
      901 ctcgggaacc cctccggcag aacggaagct gcgtattgtg ggcgaccggc ctgggtgtgt
      961 caacacgatc ccaggccggt aacggcaccc gcgccacggg aacccagcac ccccttcccc
     1021 cctcccaaaa agagcgcgtt cccacccctt tgggggggtt gttgccccgc aagcgcgaag
     1081 ttatgttcgg ccgactaccg acacacatat atatccccca accaaaggca cacgaaggga
     1141 ggtcccatga gaggacagcg tctggctatc gcaggactcg cggcactgat gttcctgccc
     1201 ggggtcgccg cggcgaccaa cggctacttc gcccacggct acggcaccgc caacaagggg
     1261 ctcgccgggg ctggcgtggc gctgccgcag aacgccctga ccatcgccac caacccggcc
     1321 ggcctgaccc ggctcggcca ccgcgtcgat ctcggcgcgg cgctgttcag tccgtggcgt
     1381 gaccactcga ttagcggctt tccgaccgat cccagcaccc tgccacccgg cagcccaccg
     1441 accttcccgt tggagacggg ctcctacgag agcggcagcg agtacttcct gatcccgcac
     1501 ttcggctaca accgcccgct cgacgagcgc agcgcgatcg gcatcgcggc ctacggtaac
     1561 ggcggcatga acaccgactg gccaggccac gccaatccaa tccgcagcca gggaggccag
     1621 ggtacagatc ccagcaatca atcgacgttc aatccttgcg cccagccaag tccgtttttt
     1681 ggcggaaccg ggacctactg caacggcggc gccggcgtcg acctgatgca gctgttcatc
     1741 accccgacct acgcccgcga ggtcagcgac agcgtcagcc tcggcgtcac cgcggtcctc
     1801 gcctaccagc gcttcgaggc gaccggtgtc gcctccttcg ccggtgtctc caacgacccg
     1861 acgaagctca gcaacaacgg ccacgaggat tcctacggcg cgggcatccg catcggcctg
     1921 ctcgcctcgc tgtcgcctac cgtcgacgtc ggcctcgcct accagagcaa gatctggatg
     1981 agcgagttcg acgactacgc cgggttgttc gccggggacg gcgacttcga cgtcccgtcg
     2041 aacttcaccg ccgggatcgc ctggaagacc agcgaccgct cgacgctggt gttcgacatc
     2101 cagcacatcc gctacagcga ggtcgacgca gtcggcaacc cgatgctgcc cgctttcatg
     2161 acctgcgcca tgcgccaaac gccgac
//
LOCUS       DBNHLM010000067         3497 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_129716, whole genome shotgun sequence.
ACCESSION   DBNHLM010000067 DBNHLM010000000
VERSION     DBNHLM010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_129716"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="AC16QF_03205"
     CDS             <1..459
                     /locus_tag="AC16QF_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6447588.1"
                     /translation="EVTYAGVEVEANAMLTDNFTAYLGFGYVDSEIDEAANTDHEGNQ
                     APLVSEYTLNVGLQYRQPLGAMNGRLELVGRVDYQRIGDTYWEPANITKRSPVDLVDL
                     RLGVEVPGDWALTAWAKNAFDKKYNAEYSPGGFLFKAPPARWGVDFTKRF"
     gene            482..1024
                     /locus_tag="AC16QF_03210"
     CDS             482..1024
                     /locus_tag="AC16QF_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6447589.1"
                     /translation="MSNAPPVNSRLHHNAYVTENMEAVRRFYEEVIGFPLIATWCEQA
                     ELFGRERTYMHCFFDVGGGESLAFFQFADPADAAEFGPELPSSPFRHIAMKVDQETQD
                     AIRDRLAAAGYKEPDTYVLNHGYCYSLYVVDPSKLIIEFTVDHEDVDQINADQRAKAH
                     AELARWLGGDHSPNNEAYHR"
     gene            1052..2086
                     /locus_tag="AC16QF_03215"
     CDS             1052..2086
                     /locus_tag="AC16QF_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen
                     decarboxylase/cobalamine-independent methonine synthase
                     family protein"
                     /protein_id="HIR6447590.1"
                     /translation="MAALESGITGSLPKPKWLSDPSAQLRADWMVADDALGEAHDDAV
                     RLAIADQVSAGLDIVTDGEQRRRHYIWGFLEALEGVDAHKLGKKQTRGARYTTELQNV
                     ARVVGDVRRSAPIFVDALKFAKAHSDRPVKVTLPGPMTIVDSVVDEHYGDALPDLAMR
                     FADILNAEARALADAGADIVQFDEPAFNVDTDAVAEWGIATLERAARGVATQRAVHIC
                     YGYGVPQVIAWKNQNQDWGHYGHTLPLLNESSIDQVSVECAASGVDVSVIQHLSNKRV
                     MLGVISVSTDEVETPELVAGRIRAGLEFCDAERLMPCTDCGLVPRPRAVAQAKMAALA
                     AGTALVRDAL"
     gene            2130..3011
                     /locus_tag="AC16QF_03220"
     CDS             2130..3011
                     /locus_tag="AC16QF_03220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6447591.1"
                     /translation="MCDEQTVRDNEAYLHTSAGMSRRQFAGAAAGAAIAMLLPRSGNA
                     LTVSGRDVRITTPDGEADAYFVHPAEGKHPGVIVWPDIRGLRPAFRQMADRLAGSGYA
                     VLVVNPFYRDARAPLVPEGASFQDEATRAVLMPLYNSITSTTTDVDAKALVDFLDAQT
                     EVDTTGGIGTMGYCMGGPMTLHSAAAVPDRVAAAASFHGSRLVTDAEDSPHLRVPGMS
                     ASFLIAIAENDDARDPQAKDVLELVFDDAGLAAEIEVYEGALHGWCPPDSSVYHEGQA
                     ERAWERLLVLFESALKS"
     gene            3083..>3497
                     /locus_tag="AC16QF_03225"
     CDS             3083..>3497
                     /locus_tag="AC16QF_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR6447592.1"
                     /translation="MKYLARAILRLGGWRIEGAKPDLEKYVLVAAPHTSNWDFLWMIC
                     MAQALEIPVSWLGKDALFHPLIGWFFQLLGGIPVDRSAPSDLVSRLASRFASSSDLVL
                     LVPVEGTRGHGLHWKSGFYHIARAAQVPVVLGFLDY"
BASE COUNT          600 a         1110 c         1175 g          612 t
ORIGIN      
        1 gaggtgacct acgccggggt cgaggtcgag gccaacgcca tgctcaccga caacttcacc
       61 gcctacctgg gcttcgggta cgtggacagc gagatcgacg aggcggcaaa taccgaccac
      121 gagggcaacc aggcaccgct ggtatccgag tacacgctca acgtcggcct gcagtaccgc
      181 cagcccctgg gcgccatgaa cggtcggctg gagctggtgg gccgggtgga ctaccagcgc
      241 attggcgata cttactggga gccggccaac atcaccaagc gcagccccgt ggatctggtg
      301 gacctgcggc tcggcgtcga ggtgcccgga gactgggcgc tcacggcctg ggccaagaac
      361 gcgttcgata agaagtacaa cgcggagtac tctccgggcg gtttcctgtt caaagccccg
      421 ccggcgcgct ggggcgtgga tttcaccaag cggttctgag ctgggcaata gcgaggacat
      481 catgagcaac gcaccaccgg tcaattcacg tctgcaccac aacgcctacg tcaccgagaa
      541 catggaagcg gtgcgcaggt tctacgagga ggtgatcggc tttccgctga tcgccacctg
      601 gtgtgagcag gccgagctct tcggcaggga gcgcacctac atgcactgct tcttcgacgt
      661 gggcggcggt gagagtctcg cgttcttcca gttcgcggat ccggcggatg ccgccgagtt
      721 cgggcctgag ctgccttcct cgccgttccg gcacatcgcc atgaaggtgg atcaggaaac
      781 ccaggacgcc attcgcgacc gcctggcggc ggccggctac aaggagccgg acacctacgt
      841 cctcaatcac ggctactgct actcgctcta cgtcgtcgac cccagcaagc tcatcatcga
      901 gttcacggtg gatcacgagg acgtggatca gatcaacgcg gaccagcgcg ccaaggctca
      961 tgcggagctc gcccgttggc tcggcggcga tcactcgccg aacaacgagg cctaccaccg
     1021 ctaggatcac gaagttcccg gagaaaggcc catggcggct ctagaaagtg gcattaccgg
     1081 cagcctgccg aaaccgaagt ggctgagcga cccttcggcg cagctgcgcg cggactggat
     1141 ggtcgcggac gacgctctcg gcgaggcaca cgacgatgct gtccggctcg ccatcgccga
     1201 tcaggtaagc gcgggcctgg atatcgtcac ggacggcgag cagcgccggc gccactacat
     1261 ctgggggttc ctggaggcgc tggagggcgt ggacgcgcat aagctgggca agaagcagac
     1321 gcgcggggcg cgttacacca ccgagctgca gaacgtcgcg cgcgtcgttg gggacgtgcg
     1381 gcggtccgcc cccattttcg tggacgctct gaagttcgcc aaggcccact ccgaccggcc
     1441 ggtaaaggtc accctgcccg gacccatgac catcgtggat tcggtggtgg acgagcacta
     1501 tggcgatgcg ctgcccgatc tcgccatgcg gtttgctgac atcctcaacg cggaggccag
     1561 agcgctcgcc gacgccggag cagacatcgt ccagttcgac gagcccgcct tcaacgtgga
     1621 caccgatgcg gtggccgagt ggggcatcgc cacgctggag cgcgcggcgc ggggcgtggc
     1681 gacgcagcgc gccgtgcaca tctgttacgg ctacggcgtg ccccaggtga tcgcctggaa
     1741 aaatcagaat caggattggg ggcactacgg gcacacgctg ccgctgctga acgagtccag
     1801 catcgatcag gtgtccgtgg agtgcgcggc gtccggggtc gacgtctcgg tgatccagca
     1861 tctgtccaac aagcgcgtga tgctgggcgt gatcagcgtg agcaccgacg aggtggaaac
     1921 gccggagctg gtggccgggc gcattcgggc cggcctcgag ttctgcgacg ccgaacgcct
     1981 catgccttgc acggactgcg gtctggtgcc gcgcccccgg gcggtggccc aggcgaagat
     2041 ggcggctctg gctgcaggca ccgcgctggt gcgggacgcc ctctgatcga ttggctcggc
     2101 cgtcgccggc cggccaggga ggcttgacca tgtgcgacga gcaaaccgtt cgtgacaacg
     2161 aagcgtatct gcatacgtct gccggcatgt cacggcgcca gttcgcgggg gccgctgcag
     2221 gtgcggcgat tgcgatgctg ctgccccgct ctggcaacgc cctgacggtt tcgggccgcg
     2281 acgttcggat caccacgccg gacggcgagg ccgatgcgta cttcgtgcat cctgccgaag
     2341 ggaagcaccc gggggtaatc gtgtggccgg acatccgggg actgcggccg gcgttccggc
     2401 agatggccga ccgcctggct ggttcgggct acgcggtgct ggtggtcaat cccttctatc
     2461 gggatgcgcg cgcgcccttg gttcccgaag gtgccagctt tcaggacgag gccacccgtg
     2521 ccgtgctgat gccgctgtac aactccatca cgtcgaccac caccgacgtg gacgccaagg
     2581 cgctcgtcga ctttctcgat gcccaaaccg aggtggacac gacgggaggg atcgggacca
     2641 tggggtactg catgggcggc ccgatgaccc tgcactcggc agctgcggtc ccggatcgag
     2701 ttgcggcggc ggcttccttt catggcagca ggctcgtcac agacgccgag gacagcccgc
     2761 acctgagagt gcccggcatg tcggcgagct ttctcatcgc cattgccgag aacgacgatg
     2821 cccgagaccc tcaggccaag gacgtcctcg agctcgtatt cgacgatgcg ggcctggccg
     2881 ccgagatcga agtctacgaa ggagcgctgc acggctggtg cccgcccgac tcgtccgtct
     2941 accatgaagg gcaggccgag cgggcctggg agcggttgct ggtcttgttc gagtcagcgc
     3001 tgaaaagctg atccgtcgcg ccatttggtg cagtgaaggg actcaggtcc tatactgccg
     3061 ggtcctcccc tcggaatctc tagtgaaata tctggctcgc gccattctgc ggctgggcgg
     3121 ttggcgcatc gagggtgcca agcccgatct tgaaaaatac gttctggtgg cggcgccgca
     3181 cacctcgaac tgggattttc tgtggatgat ctgcatggct caggcgctgg agattcctgt
     3241 gtcctggctt ggcaaagacg cgctcttcca tccccttatt ggctggtttt ttcaactgct
     3301 cggtggcatt cccgtggacc gctcggctcc ctctgatctg gtgagccggc tcgcatcccg
     3361 cttcgcgagt tcgagcgatc tcgtgctgct ggttccggtg gaaggcactc gcggacacgg
     3421 cctccactgg aaatcgggct tctaccacat tgcgcgagcc gcgcaggtgc ccgtggtgct
     3481 gggatttctc gactacg
//
LOCUS       DBNHLM010000068         5225 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_967221, whole genome shotgun sequence.
ACCESSION   DBNHLM010000068 DBNHLM010000000
VERSION     DBNHLM010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_967221"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..2147)
                     /locus_tag="AC16QF_03230"
     CDS             complement(63..2147)
                     /locus_tag="AC16QF_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR6447593.1"
                     /translation="MTYKLGVDVGGTFTDVLLIDSESGAVHTAKVPSTPEDSSVGVLN
                     GIARACANAGVDPAAITQVTHGTTVATNAVLTGRGALVGLVTTRGYRQVLQIARSYVP
                     GGLGGWVIYNKSDPMAPLECTVEVPERIGADGSVVEALDEDAARSALRSLAARGIEAL
                     TVSLINSFTNPVHEQRLAEIAAEELPRVPVSLSSEVIPEMQEYERTITTVANSYVRPE
                     VSRYIDNLQRELDERMNSPKLSILRSDGGLSTAPAAAKYPVNLLMSGPAGGVAGALWV
                     GRQAGFDNLLTFDMGGTSTDVSLIEQGKAVVRRETTVGDVNVRASSMDVRTVGAGGGS
                     IAYVPELTGALRVGPDSAGAVPGPAAYDLGGERPTVTDANVVLGYLPAEQKLGGDMTI
                     SRDKAAAAVQQIADAMGLSLEAAAEGIVKIVNENMFGALRLVSVEKGYDPRNFSLIAF
                     GGAGPLHANALGKLTQAWPVIVPPGPGVLCAYGDATTRLRDEASRTLISKLDDIEPAA
                     IADELKQLEALARQAMLAEGVGDAAAQVTDADITAQFQVDVRYSGQGLVLTVDTSEED
                     LLSDGFAKVSEAFDRLHDQLFTFALDADKEFVNLRVVVEGPEAHVSASELEPGAADPA
                     AASLGEHQIYADGQTHAGTLYDRARLHPGNVVPGPAVIMEMDSTTLILPGHHAEVDRI
                     GNLLIRPDGHDD"
     gene            complement(2150..3055)
                     /locus_tag="AC16QF_03235"
     CDS             complement(2150..3055)
                     /locus_tag="AC16QF_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HIR6447594.1"
                     /translation="MTETVIINDVGPRDGLQNDAADVTPEDRALLINRLIDAGVPAVE
                     VASFVSPRAVPKMAGADRVVAGIDLSRADCSALVPNHKGYELARDAGIRSVAVVLSAT
                     ETMNLKNINMDLETTVRVCEEVVGRAVQDGIHARAYVSVAVECPYEGLVSEETVASLA
                     GRMFDAGAAEVIVADTIGAANPAQVKSLFEKLIGAFGAERLSAHFHDTRALALANAWQ
                     ALECGIRKFDSSIGGLGGCPFAPGAAGNLATEDLASMLEQAGFHTGIDMDSLLSVVAL
                     ARELVGHDLGGRSLAWQLGRKTEAA"
     gene            complement(3088..4290)
                     /locus_tag="AC16QF_03240"
     CDS             complement(3088..4290)
                     /locus_tag="AC16QF_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6447595.1"
                     /translation="MSKPLEGIRLLDLTHMLSGPYAGMILADLGCDTVKVEPPGTGEG
                     TRRLLETDPKNSFKGMGAYFFTLNRNKRSVAIDLKSETGRRYFYELVKVADIVLDNFS
                     ADVTKRLLIDHDSLAAVNPRIVTCSVSGFGEDGPHYRRPAFDQIVQGLGGGMSITGSG
                     PGEAVRAGIPIGDLGGGMFAVMGVQAALLARERTGVGQHVDISMLDVQISLLNYMATM
                     YTLSGEVPGPIGNSHFVHTPYNTFETADDTIIIAVIGDTFWPALCDLLEIDELRKPEF
                     ATAPKRLEHKAYIEGLIGERLLTRPAAEWLEALEAARIPCARVNNIAQALDDPQILHR
                     HMVVEVEHPDGGRVKVPGNPIKLSGTPAETFSPPPLLGAHTREVFAEWAGMAEARIAE
                     GMEAGAIA"
     gene            complement(4321..>5225)
                     /locus_tag="AC16QF_03245"
     CDS             complement(4321..>5225)
                     /locus_tag="AC16QF_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6447596.1"
                     /translation="TEDFTIFGTWGKGFKAGGFNNQGSQATVDGFINGLINGDVLGGP
                     AFADILGVPLPQINDDFEEETSSAFEVGFKSQLWDGRLSLEGAAYYVEVDDMQFFEFF
                     VGTFGLLRVVSNLDEVEIYGFELAATAQLHDYVSVYAGANIIESEIKDNSARPDTVGN
                     DAPYTPDYTFNVGAELNYPLSGDLNLFARVDAQFVGDTWFHTVQSGQRPTIFSPLFEI
                     SIFGEGSGPLGFADFENSKRDAYVTVDVRAGIQGENWSVLGFAKNIGDEKYLEEVIPA
                     PEFGGAFDHPGARRRYGVEVSYRF"
BASE COUNT          903 a         1761 c         1639 g          922 t
ORIGIN      
        1 gtccagccgc gtgaacggag cgtcgttggt ctgtagtatt cgtcctgtca tgtgagcgcc
       61 cctcagtcgt cgtgcccgtc cgggcggatg agcagattgc ctatacggtc gacctcggcg
      121 tgatgccccg gaaggataag agtggtcgag tccatctcca tgatcaccgc cggccccggc
      181 accacgttac ccgggtggag tcgcgcccgg tcgtaaagcg ttcccgcgtg ggtctgcccg
      241 tcggcatata tctggtgctc ccccagggag gcggccgcgg gatcggccgc gcccggctcc
      301 aactcggacg cggaaacgtg cgcctccggc ccttccacca ccacacgcag attgacgaac
      361 tccttgtccg catccagcgc aaaggtgaag agctggtcgt ggagccggtc gaacgcctcc
      421 gacaccttcg cgaacccatc tgagagcaga tcctcttcgc tggtatccac ggtgagcacg
      481 aggccctggc cgctgtagcg aacgtcgacc tggaactgcg ccgtgatgtc ggcatcggtg
      541 acctgagccg cagcgtcccc gacgccttcg gccagcatgg cctgccgggc gagcgcttcg
      601 agctgcttga gctcgtcggc aatggccgcc ggctcgatgt cgtccagctt ggagatcagc
      661 gtccgggaag cttcgtcgcg cagcctggtg gtcgcgtcgc cataggcaca cagcacgccg
      721 ggtcctggcg ggacgatcac gggccaggcc tgggtcagct tgcccagcgc gttggcgtgc
      781 agcggccccg cgccgccgaa cgcgatgagc gagaagttgc gtggatcgta gcccttctcc
      841 accgagacca gccgcagggc gccaaacatg ttctcgttga cgatcttgac gatgccttcc
      901 gccgctgcct cgagggagag ccccatggcg tcagcgatct gctgcacggc ggccgccgct
      961 ttgtcccggc tgatggtcat gtcgccgccg agcttctgct ccgcgggcag gtagccgaga
     1021 accacgttgg catcggtgac cgtcgggcgc tcgccgccca gatcataggc ggccggcccg
     1081 ggaaccgccc cggccgaatc cggacctacg cgcagcgcgc cggtcagctc cggcacgtag
     1141 gcgatggacc cgccgccggc acccactgtg cgcacgtcca tggagcttgc ccggacgttg
     1201 acgtcaccaa cggtggtttc cctgcgcacc acggctttgc cctgctcgat gagggacaca
     1261 tccgtggacg tacctcccat gtcgaaggtc aggaggttgt cgaaacccgc ctgccggccg
     1321 acccaaagag cgccggcaac cccacctgcc ggcccgctca tgagcaggtt cacggggtat
     1381 ttggccgcag ccggagccgt ggagaggcca ccgtccgatc tcagaatcga tagcttcggc
     1441 gagttcatgc gctcgtcgag ttcccgctgc agattgtcga tgtaacgcga cacttcgggc
     1501 ctgacgtagg agttagccac ggtcgtgatg gtgcgttcgt actcctgcat ctccggaatg
     1561 acctcggatg acagggatac cggcacccgg gggagctctt ccgcggcgat ctcggcaagc
     1621 cgctgctcgt ggaccgggtt ggtgaagctg ttgatgagcg acacggtcaa ggcctcgatg
     1681 ccgcgcgcag ccagggaacg cagcgccgat ctggcggcgt cttcgtcgag cgcttcgact
     1741 accgagccgt ccgcgccgat ccgctcggga acctccaccg tgcactccag cggcgccatg
     1801 ggatccgact tgttatagat cacccagccg ccgagcccac cggggacgta ggagcgggcg
     1861 atctgcagga cctgccgata cccgcgcgtc gtgaccagac caaccagggc gccacggccg
     1921 gtgaggaccg cattggttgc cacggtggtg ccgtgggtga cctgggtgat cgccgccgga
     1981 tccacgcccg cgttggcgca cgccctggca atgccgttga gcacgcccac agatgagtcc
     2041 tccggcgtcg aaggaacctt ggccgtatgc acggcgccgc tctcggaatc gatcagcaac
     2101 acgtccgtga acgttccacc gacatctacg cccagcttgt atgtcatggt caggccgcct
     2161 ccgtctttct gcccagctgc caggcaagcg agcggccacc caggtcatga cccaccagct
     2221 cgcgcgccag cgcgaccacc gagagcaacg agtccatgtc gattcccgta tggaagccgg
     2281 cctgctcgag catggaggcc agatcttccg tggccagatt gccagcagcg cccggcgcga
     2341 aagggcagcc ccccagcccg cctatgctgg aatcgaactt gcggatcccg cattccagcg
     2401 cctgccaggc gtttgccaat gccagcgctc gggtgtcgtg aaagtgggcc gagagccgtt
     2461 cagcaccgaa ggcgccgatg agcttttcaa aaagagactt cacctgggcc ggattcgccg
     2521 cgccgatggt gtccgccacg atcacttcgg ccgcccccgc gtcgaacatg cgcccggcaa
     2581 gggacgctac tgtttcttct gagacgagac cctcgtaagg gcactccact gccaccgata
     2641 cgtaagcacg agcgtggata ccgtcctgaa cagcccggcc gaccacctcc tcgcagacgc
     2701 gcaccgtggt ctccagatcc atgttgatgt tcttcagatt catggtctcg gtggccgaga
     2761 gcaccacggc aacggagcgg atcccggcgt cacgggccag ctcgtagccc ttgtgattag
     2821 gcaccagcgc gctgcaatcg gcgcgagaga gatcaatgcc ggcaacgacc cgatccgcgc
     2881 cggccatctt cggcacggcc cgcgggctga cgaagctcgc cacctccacc gcgggcacgc
     2941 cggcgtcgat cagtcggttg atgagcaacg ctcggtcctc gggcgttacg tccgcagcgt
     3001 cgttctgcag accgtcccgg ggcccgacgt cgttgatgat tacggtttcg gtcatctttc
     3061 caatcctcgt ccagcccgct gcgcgggtca ggcaatcgcg ccggcttcca ttccttcggc
     3121 gatccgggct tcggccatcc ccgcccactc ggcaaagact tcccgggtgt gggctcccag
     3181 cagcggtggc ggcgagaacg tctccgccgg cgtgccggag agcttgatgg gattgccggg
     3241 cactttgacg cgcccgccat ccggatgctc cacctccacg accatgtgcc gatggaggat
     3301 ctgggggtca tccagggcct gcgcgatgtt gtttacccgc gcgcagggga tgcgcgccgc
     3361 ttccagggct tccagccact ccgccgccgg acgggtaagg agcctttcac cgatgagacc
     3421 ctcgatgtac gccttgtgct cgagccgctt gggcgccgtg gcgaactcag gcttgcgcag
     3481 ctcatcgatc tccagcaggt cgcacagggc cggccagaag gtgtcaccga tcaccgcgat
     3541 gatgatcgtg tcgtccgcgg tttcgaaggt gttgtacggg gtgtgcacga agtggctgtt
     3601 gccgatgggc ccgggcacct cgccggacag ggtgtacatg gtcgccatgt agttgagcag
     3661 gctgatctgc acatcgagca tggagatgtc cacgtgctgg ccaacgcccg tgcgctctcg
     3721 cgccagaagc gcagcctgca cgcccatgac cgcgaacatc ccaccgccga ggtcgccgat
     3781 ggggatgccg gcacggaccg cctcaccggg gccgctgcca gtgatggaca tgcccccgcc
     3841 gagcccctgg acgatctgat cgaaagccgg ccgccggtag tgggggccgt cttcgccgaa
     3901 cccggaaacc gagcaggtca cgatgcgggg attcaccgcc gccaggctgt catgatcaat
     3961 gagcagtcgc ttggtgacat cggcggagaa gttgtccaga acgatgtcag cgaccttgac
     4021 cagctcgtag aagtaccggc gacccgtttc cgatttgagg tcgatggcca cgcttcgctt
     4081 gttgcggttg agcgtgaaga agtacgcgcc catccctttg aacgagttct tgggatcggt
     4141 ttcgagcagg cgccgggtgc cctcgccggt acccggcggc tccaccttca cggtgtcgca
     4201 gccgagatcg gcaaggatca tgcctgcata tggacccgac agcatgtgtg tcaggtccag
     4261 caggcgaatg ccctcgagag gcttcgacat cttcgatcca gccctccccg aggttctacc
     4321 ctagaagcgg tagctcacct cgaccccgta acggcgcctg gcacccggat gatcgaacgc
     4381 gccgccgaac tccggcgccg ggatcacttc ctccagatac ttctcgtcgc cgatgttctt
     4441 ggcgaaaccc agaaccgacc agttctcgcc ctgaatgccg gcacggacgt ccaccgtcac
     4501 gtaggcgtcg cgcttggaat tctcgaagtc cgcgaagccg agaggccccg acccttcccc
     4561 gaaaatcgat atctcgaata gcggggagaa aatcgtcgga cgctgaccgc tctgaaccgt
     4621 gtggaaccag gtatcaccca cgaactgggc gtccacccgg gcaaagagat tcaggtcgcc
     4681 gctcaacggg taattcagct ccgctccgac gttgaaggtg tagtcgggtg tgtacggcgc
     4741 gtcgttccca acggtgtcgg gccgcgccga gttgtccttg atctcgctct ctatgatgtt
     4801 cgcacccgcg tagacgctga cgtagtcgtg cagctgggcc gtggcggcga gctcgaatcc
     4861 gtagatctcc acctcgtcga ggtttgagac cacccgcagg agcccgaagg tacccacgaa
     4921 gaattcgaag aactgcatgt cgtcaacctc gacgtaatag gccgcgccct cgaggctcaa
     4981 tcggccatcc cacagctggg acttgaagcc cacctcgaag gcgctggagg tctcctcctc
     5041 gaaatcatcg ttgatctgcg gcaacggcac gccaagaatg tcggcaaatg caggccctcc
     5101 gagcacgtct ccgttgatca gcccgttgat gaagccgtcg acggtggcct gactgccctg
     5161 gttgttgaac ccgccggcct tgaagccctt gccccaggtg ccgaagatcg tgaagtcctc
     5221 ggtca
//
LOCUS       DBNHLM010000069        11518 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_249055, whole genome shotgun sequence.
ACCESSION   DBNHLM010000069 DBNHLM010000000
VERSION     DBNHLM010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11518)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11518)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11518
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_249055"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="AC16QF_03250"
     CDS             complement(<1..258)
                     /locus_tag="AC16QF_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6447597.1"
                     /translation="MSALEGKVAIVSGAAQGLGEAFARALSEQGAIVTAFDVQETIGD
                     VAAAIEGATGNRVLGLVADVSKRDDVERVVAATVETFGGLDL"
     gene            complement(245..1144)
                     /locus_tag="AC16QF_03255"
     CDS             complement(245..1144)
                     /locus_tag="AC16QF_03255"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6447598.1"
                     /translation="MSVSLRGKNAIVTGAAVGIGPAYAEALAAEGVNVAVCDIREEIL
                     ELPARLEAHGVRSVGWKADVSVPEDVRKVVDGTVEAFGGIDILISNAGKCWVSVADDD
                     LDKTIADYEGMVGTNLKGEYLMGRAVIAEMLKQGRGGEIVNVATDHMVTCGSPFEICP
                     KLETCPWADSPRPTGGGDVMDVYDASKWALNGFLFAWAKALRPHGIRVNGMCMGATDS
                     WMIRDFYGFPQTPGEETEEQMEEVSTWMAKGDTAQVVMNLLKEGPEGRTAQNINLCVG
                     RPTMLEPPLPNVYITEESLNVSA"
     gene            complement(1254..2267)
                     /locus_tag="AC16QF_03260"
     CDS             complement(1254..2267)
                     /locus_tag="AC16QF_03260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6447599.1"
                     /translation="MGGKTINRQWLINGNPRGRAVALSDFVENEAELAPLGEGDVRVK
                     VEYLSFDPSQKGQLENIAGYSRGGAMGEVMYARGIGEVVESRSPGVAEGTKVAGFVGW
                     QEYASLPARSLEIVPDDDLLTARLGPLGGTGLTAYFGLLRVGRPEPGDMVVVSGAAGA
                     VGSMVGQIAEIAGCHTVGIAGGPEKCAWLVDEVGYDAAIDYKNENIKARLKELCPDGI
                     NVFFDNVGGEALNDALARIAPHARVVICGGISRYEQETLPPGPANYFNVVFRQANIEG
                     FLLSGYEAEYDVARERITEWIRSGAIVYKEDVQHGFENIPATLLRLFSGKNFGKQLLR
                     MGD"
     gene            complement(2352..4034)
                     /locus_tag="AC16QF_03265"
     CDS             complement(2352..4034)
                     /locus_tag="AC16QF_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6447600.1"
                     /translation="MTQLDEADWDLTVDLVVVGSGAAGMACALTAKLEGLDVLMVEKT
                     DRYGGTTALSGGVLWVPNNSLMKQAGVEDSAERAVTYLKHNIGNRVSAARLEAFVENA
                     PKMLDFLRARDCLEVAVFEGFPDYRPEDPGGCQGGRSVEPKVFAGRRLGDALGDLRAR
                     AAMAPAGIVGTMTELRRLAAVRSNPLELLKAWKVFPRNLWNRLSGAHHLANGRALIAW
                     LRHAMERTDVPLWLNAPLARIVTDDDGAVRGVLVRRGEEPVRVEARRGVVITAGGFEH
                     NGEMRSEYFGENTAEYSSGSPGNTGDAIRAGLDAGAAVDLMDDAWWAPTFMPPGQGPQ
                     IVIFERGKPGNIIVNAAGQRFANEADPYNEFVKHMQEADRSGASAIPSFLIFDDRYRS
                     KYPLGGMLPGVTPRRYLENGFVVRADTLVELAEIMGIDPAGLEDTVARFNDMASKGSD
                     DDYHRGDSAFDRFAGDPGVRPNPCLAALDEPPFYGMRIYPGDLGTKGGLLTNEHAQVL
                     RGDGTVVRGLYAAGNSSASAMGNFYPGAGGTIGPAMTFGYIAARHAAAEGSQ"
     gene            complement(4012..5490)
                     /locus_tag="AC16QF_03270"
     CDS             complement(4012..5490)
                     /locus_tag="AC16QF_03270"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6447601.1"
                     /translation="MGRGLTRPMFARHRSLVDASGRIPSWRLGIFASIAFPSVGMTLP
                     LAIFLPPFYTKTLGLGLAEVGLVFMLVRLFDIVTDPVMGIIGDRFDSRWGRRRHWLVI
                     ALPFMMIGVYMAFMPTGSPSIWYLGFWLVVLYAGTTMKTISHTAWAAELSPDYDQRSR
                     IAGFNSFAAYMGSLLILGPLAFLEFRGTPPAGHEALTFFGTMALVFAPICVFAAVTLV
                     GERPTAPAPRIGLIRGLRMVLRNPHMRRLLLADAMAAIPGSVMSGLFIFYQAELLGNS
                     QFNSLALIGFFVAHIIGVPLWVRLSFRVGKHRAFGISSLCFCFTTSLFFFPGEGDVAL
                     FVAFLFFTGLTHSGLQFLLRSMGADVVDYDNVQTGGQRTGLYFALLSITAKAGGALAI
                     GITYPLLAVVGFDAQGGNSEDAKFAFRMIYLVVPVMAMILAYVFIRGFKLDEAEQRAL
                     QSRIAMRDGSADGEAGAAGSADVDGDGQRAIEGGSDDATRRG"
     gene            complement(5475..6752)
                     /locus_tag="AC16QF_03275"
     CDS             complement(5475..6752)
                     /locus_tag="AC16QF_03275"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6447602.1"
                     /translation="MSDPRTCPVEGVMEVDFNSEPFLTDFKQTYAAMHAGGCPYAHST
                     AGDHYAVASHAQIVAMLKRYDLWKSKHGPGLNYQERAGVLVSVDPPEHTFEVKIVASA
                     FSVPYFESLIPEMQAFVDEQIDGFHADGRVDLHQALSIALPLFVIFRMFGIPLRDEAG
                     DKTEFVREMIIGSVGLMLTPGHQVQAELAAGSVDEARMRAIAGTQQMFVDHLADCKRK
                     LESGEYHDDSNIVCRFLTTPGPDGTYLSDAKILGFCGFLLTAGSATTTIMLSNVVYRL
                     LRQPEELAKLKANPELVPLAIEESLRLDAPVQGLFRTNDEETDLGPLHLEKDTKVMLL
                     WAGANLDPTVFDDPLRFSLDRDLAKVRRHLAFGYGTHFCRGAALARIEGEIFLRTVLE
                     RLPNLRLDGEVVMEKRMPVLQGIRSLPLAWDVD"
     gene            complement(6839..7507)
                     /locus_tag="AC16QF_03280"
     CDS             complement(6839..7507)
                     /locus_tag="AC16QF_03280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6447603.1"
                     /translation="MARGYPKIDLPVGCEPTDNIWEAMFTQRAIRYWQDKPVPRELLE
                     KVVAAGSKAPSGSNTQPWVFVVVDEPDKRAELGAALRGIFDAAEPLQQLISAGEQSDD
                     KTERLMLKGARAFFSHLEKAPAMIIPCLYGLSSPTQDATSLLAGSSIYLAVQNVLLAA
                     RALGLGTVMTTVHGMLDADLRRILSLPEDATPVALLPIGYPDANFGPTTRKGLDEILR
                     WNGW"
     gene            complement(7571..8818)
                     /locus_tag="AC16QF_03285"
     CDS             complement(7571..8818)
                     /locus_tag="AC16QF_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6447604.1"
                     /translation="MQLPTDEEWARACAADGEFGLAARHWTGGLTLAVGEAELSLRLT
                     DGAVSPGRAESAEGLLTYAGPADVWEQVLAAQPPRFHNDIMANLTVGGGLNRQVDPVV
                     HAQYYPAVMRAVELLRPAEPPFMPMRDEARPPGAFDAPVGRYVHLELEGHDHRIYFEE
                     AGQGIPLLMQHTAGCHGSQWRHLFEIPEITSRFRLIAYDLPYHGKSVPPVSREWWAER
                     YELKGDFLRSVPVKLAEALGLDRPVFMGCSVGGLLALDLALRHAEAFRAVISVEGALN
                     IEGDLGELEELWHPQVSNEYKARLMDGIMAPASPKAYRKETSFVYACGWPPVFLGDLY
                     YYINDFDIRQAAGQIDTSRVGVHILSGEYDYSGTSELGRQAHELIPGSTWTEMKGVGH
                     FPMTENPVRFVEYLEPILASISA"
     gene            complement(8911..9612)
                     /locus_tag="AC16QF_03290"
     CDS             complement(8911..9612)
                     /locus_tag="AC16QF_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR6447605.1"
                     /translation="MKEFLSARAVEFVSINVLEDREARERLARGGIRTLPVVSRDDAF
                     VPGMDLARVAEFLGLDYQGGNILPADVLVSRYHRVLDAAVRYVGQIPPARLHDTLPHR
                     DRSYLALGNHLVQIAVDFLSVTRGAALTGRLAESVPETELEPPALMAAAAQAKRGLAD
                     WLAAADSAELASPVVTYFGSQTLHQVLERAVWHSAQHGRQLMMVLQRLGISPDGPLTD
                     EDFAGLPMPEEVWDG"
     gene            complement(9662..10117)
                     /locus_tag="AC16QF_03295"
     CDS             complement(9662..10117)
                     /locus_tag="AC16QF_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447606.1"
                     /translation="MPKVVKASVWVVGLLVVAAGGYYAYLQLVGNPRVMTEIRAEPDG
                     ERARRVMLIDLPDGRTLPVNYLREDDMVYMGVDGLWWRQFRQGDVPVELLIRGEDLQG
                     HARAVLDDPDRTRRVFARLRPTAPDWLPGRMGGVLVEIRLQAATLDEED"
     gene            10292..11056
                     /locus_tag="AC16QF_03300"
     CDS             10292..11056
                     /locus_tag="AC16QF_03300"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6447607.1"
                     /translation="MSGRFDGKVVMITGATSGLGKHAAVAFAREGARVVASGRRQAEG
                     EETLAEIEAAGGTAVFVQADVSVSEDVARIVAAARDRFGGLDWAYNVAGVPGDAFAKA
                     AEYSEDAWDETMEINVKGVFLCVKHAIPAMLERGGGAIVNMSSIAGIGGSPGGVAYVA
                     SKHAIIGLTKAAALDYAEQNIRINALAPGVIYTDMLAWGITQTPGLEADLISQHPIGR
                     LGQLEEVTNAAMWLCSDEAGFVTGTTLTVDGGHLAR"
     gene            11106..>11518
                     /locus_tag="AC16QF_03305"
     CDS             11106..>11518
                     /locus_tag="AC16QF_03305"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6447608.1"
                     /translation="MNQKEIDELRQLMEYEAARSEPPEGFPSLPDLPGGRYTDQRFYD
                     LEQQHIWRKSWLLAGHIDEIPEPGCFRLWETAGQPVVLVHADSGAVNAFYNTCSHRGA
                     PVVTEPEGKRPRLVCKYHGWTYSHDGELLAIRDPE"
BASE COUNT         2035 a         3965 c         3689 g         1829 t
ORIGIN      
        1 caggtccagg ccgccgaagg tctcgacggt ggcggctacc acccgttcca cgtcgtcgcg
       61 tttcgagacg tcggccacca atccgagtac ccggttgccc gtcgctccct cgatggcggc
      121 cgccacgtcg ccgatggtct cctgcacgtc gaaggcggtg acgatggccc cctgctcgct
      181 cagcgcccgg gcaaaggctt cgccgagccc ctgggcggcg ccgctgacga tggcgacttt
      241 tccctcaagc gctgacattg agactctcct cggtgatgta gacgttgggc aacggcggct
      301 cgagcatggt cggccggccc acgcagaggt tgatgttctg ggccgtgcgg ccctctgggc
      361 cttccttgag caggttcatc accacctggg ccgtatcccc cttggccatc caggtggaca
      421 cctcctccat ctgctcctcg gtctcctccc ccggcgtctg cgggaagccg tagaagtccc
      481 ggatcatcca ggagtccgtc gcgcccatgc acatgccgtt gacgcggata ccgtggggac
      541 gaagcgcctt ggcccaggcg aatagaaatc cgttcagcgc ccacttggac gcatcgtaga
      601 cgtccatcac atcacccccg cccgtgggcc ggggcgaatc tgcccaggga caggtctcga
      661 gcttcgggca gatctcgaaa ggcgagccgc aggtgaccat gtggtcggtg gccacgttga
      721 cgatctcgcc gccgcggccc tgcttgagca tctcggcgat caccgctctg cccatgaggt
      781 attcgccttt gaggttggtc cccaccatcc cctcgtagtc ggcgatcgtc ttgtccaggt
      841 cgtcgtcggc cacgctgacc cagcacttgc cggcgttgct gatcaggatg tcgatgccgc
      901 cgaaggcttc gaccgtcccg tccaccacct tgcgcacgtc ctcgggcacg gacacgtcgg
      961 ccttccagcc tacggaccgg acgccatggg cctccaggcg ggcgggcaac tcgagaatct
     1021 cttcacgaat gtcgcaaacg gcgacgttga ccccttcagc ggcaagcgct tccgcatagg
     1081 ccggaccaat gccgacggcg gcgcccgtaa cgatggcgtt cttgccacgc agtgaaactg
     1141 acatcatccc ccctcatcga gatgtgattg aactcccgcc acgagaatac acgaaggctt
     1201 cagtctgcgt cagcgtgccc caaccgggga tcgatccggc cggagacggc acctcagtcg
     1261 cccatcctga gcagctgctt gccgaagttc ttgccggaga acagccgcag cagcgtcgcc
     1321 ggaatgttct cgaagccgtg ctggacgtct tccttgtata cgatggcccc ggaacggatc
     1381 cattcggtga tgcgttccct ggcgacgtcg tactcggctt cgtagccgct cagcagaaag
     1441 ccttcgatgt tggcctgcct gaacacgacg ttgaaatagt tggcaggacc cggcggcagg
     1501 gtttcctgct cgtaccggga tatgccgccg cagatgacga ctctggcgtg gggcgcgatc
     1561 cgcgccagcg catcgttgag cgcctccccg ccgacgttgt cgaagaaaac gttgatcccg
     1621 tccgggcaca gctccttcag gcgggctttg atgttctcgt tcttgtagtc gatggcggcg
     1681 tcgtagccga cctcgtccac gagccatgcg cacttttccg ggccgcccgc gatcccaacg
     1741 gtgtggcagc cggcaatttc ggctatctga ccgaccatcg aacccacggc cccggccgca
     1801 cccgacacca ccaccatgtc gccgggttcc ggccggccca cccgaagcag cccgaagtag
     1861 gcggtgaggc cggtgccgcc cagcggaccg agccgggcgg tgagcagatc atcgtcgggc
     1921 acgatctcca ggctgcgagc cggcagcgac gcgtactcct gccagcctac gaacccggca
     1981 accttcgtgc cctccgccac gcccgggctg cgcgactcca ccacctcgcc aatgcccctg
     2041 gcatacatca cctcgcccat ggcgcctccc cgggaatagc cggcgatgtt ctccagctgc
     2101 cccttctggg acgggtcgaa gctcaggtac tccaccttta cccggacgtc gccttctccc
     2161 aggggcgcaa gctccgcctc gttctccacg aagtcgctca gcgccacggc tctgccgcgc
     2221 gggtttccat tgatgagcca ctgtctgttg atggtcttcc cacccatttg ctttcccctg
     2281 acatcaaatc cgtctgcgcg acgggcccca cccgcagcgc ccccgcgccg tcacgatggt
     2341 actttaaccg cctactggct accctcggcg gcggcgtgcc gggcggcgat gtaaccaaag
     2401 gtcatcgccg gcccgatggt cccgccggca ccggggtaga agttacccat ggccgaggcc
     2461 gagctgttcc ccgcggcata caggccgcga accacggtgc cgtcgccacg cagaacctga
     2521 gcgtgctcgt tggtgagcaa cccgcctttg gtaccgagat cgcccgggta aatccgcatg
     2581 ccgtagaagg gcggctcgtc cagggcagcc aggcaggggt tgggtcgtac cccgggatca
     2641 ccggcgaagc ggtcgaacgc gctatcgccc cggtgatagt cgtcgtcgct gcccttcgag
     2701 gccatgtcgt tgaacctggc caccgtatct tcgagaccgg ccgggtcgat ccccatgatt
     2761 tcggccagct cgaccagggt gtccgcccgc acgacgaacc cgttttccag atatcgccga
     2821 ggcgtgacgc cagggagcat gccgcccagg ggatacttgc tccggtaacg gtcgtcgaag
     2881 atgagaaacg acgggatggc ggaagccccg ctgcgatccg cctcctgcat gtgcttgacg
     2941 aactcgttgt agggatcggc ctcgttggca aagcgctggc ccgccgcgtt cacgatgatg
     3001 ttacccggct tgccgcgctc gaaaatgacg atctgcggtc cctggccagg cggcatgaag
     3061 gtgggcgccc accaggcgtc gtccatgagg tctaccgcgg cgccggcgtc gagacctgcg
     3121 cggatggcgt ctcccgtgtt gccaggcgag ccggatgagt attcggccgt gttctcgccg
     3181 aagtactccg agcgcatctc gccgttgtgc tcgaaccctc ccgccgtgat caccacgccg
     3241 cgcctggctt ccacccgcac gggctcttcg ccacgcctga ccagcacgcc tcgaacggca
     3301 ccgtcatcgt cggtgacgat gcgcgcaagc ggcgcgttca gccacagcgg aacgtccgtg
     3361 cgttccatgg cgtggcgaag ccatgcgatg agggcgcgcc cgttggcaag gtgatgggct
     3421 ccggagagac ggttccacag gttccgcggg aagactttcc aggccttgag cagctccagc
     3481 gggttggagc gcacggcggc gaggcgcctc agctccgtca tggtgccgac gatgcctgcg
     3541 ggcgccatgg cggcgcgggc gcgcaggtcg cccagcgcat cgcccagcct gcggccggcg
     3601 aagaccttcg gctctaccga ccggccgccc tgacagccgc ccggatcttc cggccggtaa
     3661 tcgggaaaac cttcgaaaac ggccacttcc agacaatcgc gcgcgcggag aaagtcgagc
     3721 atcttcggcg cgttctcaac gaacgcctcg agcctggccg cgctgacccg gttgccgatg
     3781 ttgtgcttca gatacgtcac ggcacgctcg gccgagtcct cgacgcccgc ctgcttcatc
     3841 agcgagttgt tgggcaccca cagcacgcca ccggaaagcg ccgtggtgcc gccgtaacgg
     3901 tccgtcttct cgaccatcag aacatccagg ccctcgagct ttgccgtcaa ggcacaggcc
     3961 atccccgccg ccccggaccc caccaccacg agatcgacgg tgaggtccca gtcagcctcg
     4021 tcgagttgcg tcatcgctgc ctccctctat cgcccgctgg ccgtccccgt cgacgtcagc
     4081 cgatccggcc gcccctgctt ccccgtccgc cgatccgtca cgcatcgcga tccgggactg
     4141 cagcgcccgc tgctccgcct cgtcgagctt gaatccccga atgaagacgt aggcgaggat
     4201 catggccatg acgggcacca cgaggtagat catacggaaa gcgaacttcg cgtcctccga
     4261 atttccaccc tgggcatcga aacccaccac cgcaagcagc ggataggtaa tgccgatggc
     4321 gagcgccccg cccgccttgg cggtaatgga gagcagcgcg aagtaaaggc ccgttcgctg
     4381 cccgcccgtc tgcacgttgt cgtagtccac cacgtccgcc cccatggacc gcagcagaaa
     4441 ctgcaggccc gaatgggtca aacccgtgaa gaacagaaac gccacgaaca gcgcgacgtc
     4501 gccctcgccc gggaagaaga acagcgacgt ggtgaagcag aagcacagcg agctgatgcc
     4561 gaacgcgcgg tgcttgccga ctctgaacga caggcgtacc cagagcggca cgccgatgat
     4621 gtgagccacg aagaagccga tgagcgcgag cgagttgaac tgggagttcc ccagcagctc
     4681 cgcctgatag aagatgaaca gccccgacat caccgaaccg ggaatggctg ccatggcatc
     4741 ggcaagcagc aggcggcgca tgtgggggtt ccgcagaacc atgcgcaggc cgcgaatcag
     4801 cccgatgcga ggcgccggcg ccgtggggcg ctcgccaacc agggtgaccg ccgcaaacac
     4861 gcagattggc gcgaacacca gcgccatggt gccgaagaac gtcaacgcct cgtgccctgc
     4921 cggcggcgtt ccccgaaact cgagaaacgc cagggggccc aggatgagca gcgagcccat
     4981 gtaggccgca aacgagttga agccggcgat gcgcgatcgc tggtcgtaat cgggggacag
     5041 ctccgccgcc cacgccgtgt gggagatggt cttcatggtc gtgcctgcat agagcaccac
     5101 cagccagaag cccagatacc agatcgaggg cgacccggtc ggcatgaacg ccatgtagac
     5161 gccgatcatc atgaagggca gcgcgatcac cagccagtgc cggcgccggc cccagcggct
     5221 gtcgaagcgg tcgccgatga tgcccatcac cgggtcggtg acgatgtcga acaggcgcac
     5281 gagcatgaac acgagaccca cctcggcaag gcccaggccc agggtctttg tgtagaacgg
     5341 cggcaggaag atggccagcg gcagcgtcat gccgacgctg gggaacgcga tggaggcgaa
     5401 gatgcccagc cgccaggagg ggatgcgccc ggaagcgtcg acgagagacc gatggcgggc
     5461 gaacatgggc cgcgtcagtc cacgtcccac gccagcggca gggagcggat tccctgcagc
     5521 accggcatac gcttctccat gaccacctcc ccgtccagcc tcagattggg cagccgctcc
     5581 agcaccgtgc gcaggaatat ctcaccctcg atccgggcaa gcgccgcgcc gcggcagaag
     5641 tgggttccgt acccgaaggc cagatggcgg cggaccttgg cgagatcccg gtccaggctg
     5701 aaacgcagcg ggtcgtcgaa gaccgtcggg tcgaggttgg cgcccgccca cagcagcatg
     5761 accttggtgt ccttctccag gtgcagaggt cccaagtccg tttcctcgtc gttggtgcga
     5821 aacaggccct gcacgggcgc gtcgaggcgc agggactctt cgatggcaag cggcacgagc
     5881 tcggggttgg ccttcagctt ggcgagctcc tcgggttgcc tgagcaggcg gtagacgacg
     5941 ttggacagca tgatcgtggt cgtcgcgctc cccgccgtca gcagaaagcc gcagaaaccg
     6001 aggatcttcg catccgacag gtaggtgccg tccggacccg gcgtggtcag aaaacggcag
     6061 acgatgttgc tgtcatcgtg gtactcgccg gattcgagct tgcgtttgca gtccgccaga
     6121 tggtctacga acatctgctg ggtaccggca atggctctca ttcgggcctc gtccacggac
     6181 ccggcggcga gctcggcctg cacctggtgg ccgggcgtca gcatcagccc gacggagccg
     6241 atgatcatct cgcgcacgaa ctccgtcttg tcgccggcct cgtcgcgcag cgggatgccg
     6301 aacatccgga agatcacgaa cagcggcagc gcgatggaca gcgcctgatg cagatccacc
     6361 cgcccatcgg catggaaacc gtcgatctgc tcatccacga acgcctgcat ctcgggtatg
     6421 agggactcga agtagggcac cgagaaagcg ctggcgacga tcttcacctc gaaggtgtgc
     6481 tccggcggat ccacgctgac gagcacgccc gcgcgctcct gatagttgag accggggccg
     6541 tgcttgctct tccaaagatc gtagcgcttg agcatggcga cgatctgggc atgggacgcc
     6601 acggcgtagt gatctcccgc ggtggaatgc gcgtaagggc agccgcccgc gtgcatggcg
     6661 gcgtaggtct gcttgaagtc ggtgaggaag ggctcggagt tgaaatccac ctccatgacc
     6721 ccttccaccg gacaggtcct gggatcgctc atggggtgcc cccggcgcgc accgcggcgc
     6781 cgcggtcacg gggccggcgc agccgcagcc gccgacgggt agccaggggc ctcacgatct
     6841 accagccgtt ccagcgcagg atctcgtcga gccccttgcg ggtcgtgggt ccgaagttgg
     6901 cgtcggggta gccgatgggg agcagcgcca cgggcgttgc gtcctcgggc agcgacagga
     6961 tccgccgcag atcggcgtcg agcatcccgt ggacggtggt catcacggtg ccgagcccaa
     7021 gcgcccgagc cgccagcagc acgttctgca cggccaggta gatcgagctt ccggccagca
     7081 gcgacgtggc gtcctgagtg ggcgaggaca agccgtagag gcagggaatg atcatggcgg
     7141 gcgccttctc cagatgggag aaaaacgctc tggcgccctt gagcatcagc cgctcggtct
     7201 tgtcgtcgga ttgctccccg gcggagatca gctgctgcaa gggctccgct gcgtcgaaaa
     7261 tcccccgcag tgctgccccg agctcggccc gtttgtccgg ttcgtcgacc accacgaaca
     7321 cccacggctg ggtattcgag ccgctcggcg ccttggaccc ggcggccacg actttctcca
     7381 gcaattctct gggaacgggc ttgtcctgcc agtagcggat ggcccgctgg gtgaacatgg
     7441 cctcccagat gttgtcggtc ggctcgcaac ccaccggcag atcgatctta ggatatcctc
     7501 tcgccatgct ccccccttcc ctgctcggcc ggatggttca gagtagagca gttttccagc
     7561 gggaatgaaa ctaagccgag atcgaggcga gtatcggttc caggtactcg acgaaccgca
     7621 cgggattctc ggtcatgggg aagtggccga cccccttcat ctccgtccag gtggaacccg
     7681 ggatcagctc gtgggcctgc cggcccagct cggaggtgcc gctgtagtcg tactcgccgg
     7741 agaggatgtg cacgcccacc cggctggtgt cgatctgccc cgctgcttgc cggatgtcga
     7801 agtcgttgat gtagtagtac agatcgccca gaaaaaccgg cggccacccg caggcgtaaa
     7861 cgaagctcgt ttccttgcgg taagccttcg gcgaggcggg cgccatgatc ccgtccatga
     7921 ggcgggcctt gtactcgttg ctcacctgag ggtgccagag ctcctccagc tcgcccagat
     7981 cgccctcgat gttgagcgcg ccttccacgg agatcacggc ccggaacgcc tcggcgtgtc
     8041 gcagcgcgag gtccagagcc agcagcccgc ccacggagca tcccatgaaa acgggccggt
     8101 ccaaaccgag cgcctcggcc agcttgaccg gcaccgagcg caggaaatct cccttgagct
     8161 cgtagcgctc cgcccaccac tcgcggctga cgggggggac cgacttgccg tgatacggca
     8221 ggtcgtaggc gatgaggcgg aagcggctgg tgatctcggg aatctcgaag aggtggcgcc
     8281 actggctgcc gtggcagccg gccgtgtgct gcatcagcag ggggatgccc tgccccgcct
     8341 cctcgaagta gatccggtga tcgtggccct ccagctccag atggacatag cgccccacgg
     8401 gggcgtcgaa ggccccgggc ggtcgggcct cgtcgcgcat gggcatgaac ggcggctccg
     8461 ccggccgaag cagctccacg gcgcgcatca ctgccgggta gtactgagcg tgaaccaccg
     8521 ggtcgacctg gcggttgagc ccgcccccca cggtcaggtt ggccatgatg tcgttgtgaa
     8581 accgcggcgg ctgggccgcg agcacttgct cccatacgtc ggcagggccc gcgtaggtca
     8641 gcagaccttc ggccgattcg gccctgcccg gagacacggc gccgtccgtc aggcgaaggc
     8701 tgagctccgc ctccccgacg gcgagggtga gaccccccgt ccagtgccgg gccgccagac
     8761 cgaactcgcc atccgccgcg cacgcgcggg cccattcctc gtcggttgga agctgcattc
     8821 ccccgttctc ctccccgttc gactcgccgc tgggcgaccg tcaccggacg cccgccatca
     8881 tcataacccc acgcaccacc gccgcggcgg tcagccgtcc cagacctcct ccggcatggg
     8941 cagccctgcg aaatcctcgt ccgtcagcgg cccgtccggc gagatcccaa gccgttgcag
     9001 aaccatcatc agctgacgcc catgctgggc cgagtgccac accgcccgtt ccagcacctg
     9061 atggagcgtc tgggaaccga agtacgtcac cacaggtgaa gcgagctcgg cactgtccgc
     9121 tgccgccagc cagtccgcca ggccgcgctt cgcctgggcg gcggcggcca ttagtgctgg
     9181 cggctccagc tcagtctccg gcaccgactc ggcaagccgg ccggtgagcg cagcaccccg
     9241 ggtcaccgac aggaaatcca ccgcgatctg gacgagatga ttgcccaggg caaggtagga
     9301 acggtcccga tggggcagcg tgtcgtgcag gcgggcgggc ggtatctgcc ccacgtatcg
     9361 caccgcggca tcgagcaccc ggtgataccg gctgacgagc acgtctgccg gcaggatgtt
     9421 gccgccctga tagtccaggc ccaggaattc ggcgacccgc gcgagatcca tcccgggcac
     9481 gaaagcgtcg tcgcgggaaa ccacaggcag ggtccggata cccccacggg caaggcgctc
     9541 acgcgcttcc cggtcctcca gcacgttgat ggaaacgaac tcgaccgcac gggccgatag
     9601 aaactccttc acccggagac agctggttca cccgggctgc cagtagacgc aggtccccac
     9661 gtcagtcctc ctcatcgaga gtcgccgcct gcagccggat ctcgaccagc acgccgccca
     9721 tccggcccgg aagccagtcc ggcgcggtgg gtcggagccg ggcgaagacc ctgcgggtcc
     9781 ggtcgggatc gtcgagcacg gcacgggcgt ggccctgcag atcttcacca cggatgagca
     9841 gctcaacggg cacatcgccc tggcgaaact gccgccacca caacccgtcc acccccatgt
     9901 agaccatgtc gtcctcacgc aggtagttga ccggcagggt ccggccgtcg ggcaggtcga
     9961 tgagcatgac gcgccgtgcc cgctcgccgt ccggctcggc ccggatttcg gtcatcaccc
    10021 ggggattgcc gacgagctgc aggtacgcat agtagcctcc ggccgcaacg accagcagcc
    10081 cgacgaccca caccgacgct ttgaccactt tcggcaagtc cgacccgccc cgggaactca
    10141 gagcacgtag cctgacacag ggcagcgcaa agctgaaccg ccgggcgctc cggcagactc
    10201 tcgcccgggg ccgtggaatc cggccttgat gagatagcgg gtacgcaggg cctcgggcac
    10261 aattggcgcc agagtgcttg gggagcaaac gatgagcgga cgcttcgacg gcaaggtcgt
    10321 catgatcacc ggcgccacgt ccggtctggg taagcacgcg gccgtcgcct tcgcccggga
    10381 aggggccagg gtcgtagcgt ccggacgacg ccaggcggaa ggcgaagaga ccctcgcgga
    10441 gatcgaagcc gccggcggca cggccgtgtt cgtccaggcc gacgtcagcg tgagcgagga
    10501 cgttgcgcgc atcgtggccg ctgcccggga ccgattcggc gggctcgact gggcctacaa
    10561 cgtggccggc gttcccggcg acgcctttgc caaggcggca gagtattccg aggacgcctg
    10621 ggacgagacc atggagatca acgtcaaagg cgtttttctc tgcgtaaagc acgccatccc
    10681 ggccatgctc gagcgcgggg gcggcgccat cgtcaacatg tcgtccatcg cgggaatcgg
    10741 cggctcgccg ggcggcgtgg cttacgtggc gtccaagcac gccatcatcg ggctcaccaa
    10801 ggccgcagcg ctggactacg ccgagcagaa catcaggatc aacgccctgg cgccgggcgt
    10861 gatctacacc gacatgctcg cctggggaat cacccagacg cccggcctgg aggccgatct
    10921 gatctcacag catccgatcg ggcggctcgg acagctagag gaagtgacaa acgcggccat
    10981 gtggctgtgc tcggacgagg ccggcttcgt cacgggcacc accctgaccg tggacggcgg
    11041 gcacctggcc cgctaaaacg aagcatcgta gaaccatcga tggcacgacg agagaggctg
    11101 aatccatgaa ccagaaagaa atcgacgagc tgcgccagct catggagtac gaggcggcac
    11161 gaagcgaacc gcccgaaggt tttccgtcac tgcccgacct gccgggcggt cgttacaccg
    11221 atcagcgctt ctacgacctg gagcagcagc acatctggcg aaagtcgtgg ctgctcgccg
    11281 gtcacatcga cgagattccg gaacccggct gtttccgcct ctgggagacc gcgggccagc
    11341 ccgtggtgct cgttcatgcg gacagcggcg cggtgaacgc cttctacaac acgtgcagcc
    11401 accgcggcgc gcccgtcgtc accgaaccgg aaggcaaacg cccgcgtctg gtgtgcaaat
    11461 atcacggctg gacctacagc cacgacggcg agcttctggc catccgcgat cccgagga
//
LOCUS       DBNHLM010000070         7982 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1027404, whole genome shotgun sequence.
ACCESSION   DBNHLM010000070 DBNHLM010000000
VERSION     DBNHLM010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7982)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7982
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1027404"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="AC16QF_03310"
     CDS             <1..1028
                     /locus_tag="AC16QF_03310"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HIR6447609.1"
                     /translation="YFTTCGWMMWNWLVSGLATGATLVLYDGSPFHPGPEALLDMAEQ
                     EDVTVFGVSARYIAALQKTSLKPMETHDLSPLRAILSTGSPLTHEGFRYVYEHVKRDV
                     LLASISGGTDIISCFVLGNPTLPVWEGEIQCRGLGMAVEVVDDGGRPVTGEKGELVCT
                     RPFPSCPLGFWNDTDGAKFRAAYFQRFPGIWAHGDFAEVTEHDGFVIHGRSDAVLNPG
                     GVRIGTAEIYRQVEHVDAVLESLCVGQEWEGDTRVVLFVVMRPGRELDEALAQQIRQR
                     IRQHASPRHVPARIVQVADIPRTLSGKIVELAVRETIHGRPVQNTSALANPEALDLYS
                     NLPELES"
     gene            complement(1082..1930)
                     /locus_tag="AC16QF_03315"
     CDS             complement(1082..1930)
                     /locus_tag="AC16QF_03315"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6447610.1"
                     /translation="MSEQGREGLQIKPVGGALGAEVTGVDLCQRLPDEHFETIRNAFA
                     EHGVLFFRDQKLAPDDQVRFAERWGPININRFFRPVDGYPMIAEVRKEPDQRVNIGGG
                     WHTDHSYDDVPAMGSLLYAIDVPEQGGDTQFASMYAAYDALSDGLKEMLGRLRAVHSS
                     RHVFGSAHPADPSGRIGNPELAVQDAVHPVIITHPLSGRRALYVNPGFTVRFEDWTEE
                     ESKPLLLYLYQHASRAEFTYRFRWQPGSLALWDNRATWHYAVNDYHGKRRLLHRITIE
                     GEPLGP"
     gene            complement(1976..2506)
                     /locus_tag="AC16QF_03320"
     CDS             complement(1976..2506)
                     /locus_tag="AC16QF_03320"
                     /inference="COORDINATES: protein motif:HMM:NF049802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2228..2230),aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HIR6447611.1"
                     /translation="MTIIMDPTDERVPVARQLTPRSGAVAGSVALLDISKPRGSVLLD
                     RLEELLKERLPGIVVNRYAKPTFTKPAPETLRREIFASNDFVVEGLADUGSCTTCSLH
                     DTVWFEIQGKPAVSIASSEFGDAADTQARALGMEDACCVFVPHPIQDATDDEMRAKAD
                     GVVEAVIEALTVGGSG"
     gene            complement(2507..4090)
                     /locus_tag="AC16QF_03325"
     CDS             complement(2507..4090)
                     /locus_tag="AC16QF_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR6447612.1"
                     /translation="MSALPAFLLQDEEGNAHTFPGSRGALVCFVKEDCPTCNEVMPVL
                     DALNRSVDGALDMLVAGQTREGNRRLKEQHGPGFPILDDSTLKVSFAFDIDTVPTLFL
                     CDAQGVPERTLVGFVRDEWRELAELLADRAGASAPSIDWDALPEWRPGCGSLSVDPVI
                     ADRLRAEAENGPLRARRIEIASQDDEFEFMFDQGFSDGLPLIPPTPERVVRMLSGTSR
                     DPQEVVGVVAPNLGEATVEKVAINAVMAGCRPEYMPVVLAAMEAVCTDEYNIHGVMAT
                     TMGASPVMVVNGPVRHRLGMNMGLGALGQGSRANATIGRAVRLAIRNIGGARPGGTER
                     STLGNPMKFTMCFAEWEERNPWSPLHVERGFAPEDSVVTVFTMTSGPTLMVDQDSRSA
                     AQLAGSLGLCLEAAFHPKAHYATEVLVVLCPEHIDTLLRDGFDKAAIRSRIQEVTARP
                     IAELAEDPVSAVGFKKAAVDAMTPEQRARLLPKFRRDEDIHLVVAGSDAGKFSGAFHG
                     WVTGEIGSISVSRKIEEAG"
     gene            4222..4563
                     /locus_tag="AC16QF_03330"
     CDS             4222..4563
                     /locus_tag="AC16QF_03330"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR6447613.1"
                     /translation="MLLRSLRVARQAFEEGTGWKLTSEGACKGEVCIPLSKPEGTVVD
                     VAELAETLNMPLVGEPERNLWALGPEAIGGRALTTALAPELTLPDLDGNEFTLSSLRG
                     QKILVYAWAPY"
     gene            4576..5319
                     /locus_tag="AC16QF_03335"
                     /pseudo
     CDS             4576..5319
                     /locus_tag="AC16QF_03335"
                     /inference="COORDINATES: protein motif:HMM:NF041054.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ResA-like WAxxUGC motif-containing protein"
     gene            5351..6529
                     /locus_tag="AC16QF_03340"
     CDS             5351..6529
                     /locus_tag="AC16QF_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6447614.1"
                     /translation="MSELSEFRSDVAAWLNENCPPGARGRGQIPTGSSKVELEPDVKL
                     WLERCAERGWTAPTWPTEYGGGGHSAGETKIIYEEMAEIGARAPLMGMGLSMIGPTLL
                     EFGTPDQKERHVPSITRGEVHWCQGYSEPNAGSDLAALQTRAEDRGDHFVINGQKIWT
                     SGATLADWMFALVRTDFDAPKHEGISFVLLTMDQPGVTIKPIRLISGNSPFCETFFDD
                     AVASKADLVGELNKGWSVGKRLLQFERSGIGGLGGANARANPGSGLAEMARRFAGGAP
                     GRIQDPDVRQAVTRFNMNRAAFRLTTQRASEENRSGTPGAATSIFKLYGAALQQDAAE
                     LRRVFLGTQGLGADEEAFSDDELETTREWLATRATTIYGGTNEVQANIIAKRVLGLPD
                     "
     gene            complement(6571..7569)
                     /locus_tag="AC16QF_03345"
     CDS             complement(6571..7569)
                     /locus_tag="AC16QF_03345"
                     /inference="COORDINATES: protein motif:HMM:NF048461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR6447615.1"
                     /translation="MPFNEFHSLPPGLLECEARELEGALGLPSLIHLPGGREPALFVS
                     VLLHGNEPSGWNGLRRLLADHPELPRSMSIFVGNVQAARAGMRALPGQQDFNRVWRGA
                     GGVEGEMARGVAGRLAERRFLAAVDLHNNTGHNPHYSVLSDLSVENLGMAYLFCDKAV
                     LIEEPDTVITHLFAGRCPAVTLELGPVSDPECEIRAYDYLLRCLRLDAVPAGHAEDFS
                     LYRSEARVHVPLGVSFHFADEGGAAPLVLTGGVEGVNFHELPAGFRFGSSEGRLEDVL
                     RVLDGAHQDVTERYFALQDGAILLKRPVVPAMYTTDPYVVHQDCLCYLMQRLEHAP"
     gene            complement(7573..>7982)
                     /locus_tag="AC16QF_03350"
     CDS             complement(7573..>7982)
                     /locus_tag="AC16QF_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447616.1"
                     /translation="AFYFGLIRGFGLQDEPVESRLPFEVARDNFYNCARYGLNGRVRW
                     IRDGREEELLVRSLIMEDLLPLARRGLQSQDIPGEEIEAYLDVVAARVDSGQNGAAWQ
                     RRWVSLNGPDLHGMVGAYRQQQDAGEPVHTWSL"
BASE COUNT         1466 a         2688 c         2529 g         1299 t
ORIGIN      
        1 tctacttcac cacctgcggc tggatgatgt ggaactggct ggtcagcggg ctcgccaccg
       61 gtgccacgct ggtactctac gacggctcgc cctttcatcc gggtcccgaa gccctgctgg
      121 acatggccga gcaggaagac gtcacggtgt tcggcgtcag tgcccgctac atcgccgccc
      181 ttcagaagac ttcgctcaag cccatggaga ctcacgatct ttcgccgctg cgagccattc
      241 tgtcgaccgg ctcgccactg actcacgaag gcttccgcta cgtctacgag cacgtcaagc
      301 gggacgtgct gctcgcttcg atttccggcg gcaccgacat catttcctgc ttcgttctgg
      361 gcaaccccac gctgccggtt tgggaaggcg agattcaatg ccgggggctc ggcatggcgg
      421 tcgaggtggt ggacgacggc ggccgaccgg tcaccgggga aaagggcgag ctcgtctgca
      481 cccggccgtt tccctcctgc cccctcggct tctggaacga cacggacggc gcgaagttcc
      541 gtgccgctta cttccagcgt ttccccggca tctgggctca cggcgatttc gccgaggtca
      601 ccgagcacga cggcttcgtc attcacgggc gcagcgacgc cgtgctgaat ccgggaggcg
      661 tcaggatcgg cacggccgag atctaccgtc aggtagagca cgtcgacgcg gtgctcgaaa
      721 gcctgtgcgt cggccaggag tgggagggag acacccgcgt cgtgctcttc gtcgtgatgc
      781 ggcccggccg cgagctggac gaagcgctgg ctcagcagat acgccagcgc attcgtcagc
      841 atgcatcgcc ccgtcacgtt cccgcgcgca tcgtgcaggt ggccgatatc ccccgaacgc
      901 tgagcggcaa gatcgtagag ctggcggttc gagagaccat ccacgggcgc cccgtgcaga
      961 acacgtcggc tctcgcgaac ccggaggccc tagatctcta tagcaacctg ccagaactcg
     1021 aaagctgagg ccgcccacgc ccgccagccc gatctttgcg ccccctcttg gttccgtccc
     1081 ctcaagggcc cagcggctct ccttcgatgg tgatccgatg cagcaggcgt cgcttgccgt
     1141 ggtagtcgtt gacggcgtaa tgccaggttg cacggttatc ccagagggcc aacgaaccag
     1201 gctgccaacg gaaccggtac gtgaactcag cacgcgacgc atgctggtac aggtagagca
     1261 gcagcggctt cgactcctcc tcggtccagt cttcgaagcg aacggtgaat cccggattga
     1321 cgtaaagggc ccgacggcct gacaggggat gcgtgatgat gaccgggtgc acggcatcct
     1381 gaaccgcgag ctccggattg ccgatgcgac cgctcggatc cgccgggtgc gcgctgccaa
     1441 acacgtgacg gctggagtga acggcccgca gccggccaag catctccttg agcccgtccg
     1501 acagcgcgtc gtaggccgcg tacatgctgg caaactgggt gtcaccgccc tgttccggaa
     1561 cgtcgatggc atacagcagc gaccccatgg ccggcacgtc gtcgtatgag tgatccgtgt
     1621 gccagccacc gccgatgttg acgcgctgat ccggctcctt gcgaacctcg gcgatcatcg
     1681 ggtacccgtc caccggccgg aagaatcggt tgatgttgat cggcccccag cgttcggcga
     1741 agcgaacctg gtcatcgggc gcaagcttct ggtcgcgaaa gaacagcacc ccgtgttccg
     1801 caaacgcgtt gcggatcgtc tcgaagtgct cgtcgggcag tcgctggcag aggtccacgc
     1861 cggtgacctc ggcgccaagc gcgccgccaa cgggctttat ctgcaatcct tcacgccctt
     1921 gctcgctcat cgcgcgttcc tgcccccctt tcgcccgggg cgattcgaat ctcaactaac
     1981 ccgagccgcc caccgtcagg gcctcgatga cggcctccac gacgccgtcg gcctttgcgc
     2041 gcatctcgtc gtcggtcgcg tcctggatcg ggtgcggcac gaagacgcag caggcgtcct
     2101 ccatcccgag cgcgcgcgcc tgggtgtccg ctgcgtcacc gaactcgctg gacgcaatgg
     2161 aaaccgcggg ctttccctga atctcgaacc ataccgtgtc atgcaaactg cacgtggtac
     2221 aggatcctca gtcggcaagc ccttcgacga cgaaatcgtt gctcgcgaat atctcacggc
     2281 gcagcgtttc aggcgccggc ttggtgaacg tcggcttcgc gtagcggttg accacgatgc
     2341 ccggcagacg ctctttcaac agctcctcca accgatcgag cagcacgctg ccacgcggct
     2401 tggagatgtc cagcagcgcc acgctgcccg caaccgcgcc gcttctcggc gtcagctgcc
     2461 gggcgacggg cacgcgctcg tcggtgggat ccatgatgat ggtcattcaa cctgcctcct
     2521 ctattttccg ggatacggaa atggaaccaa tctctccagt aacccagcca tgaaaggccc
     2581 cggagaactt accggcatca gagccggcga cgacgagatg aatgtcctca tcccgcctga
     2641 atttcggcag caacctcgcc ctctgctccg gcgtcatggc gtcgacggcc gctttcttga
     2701 acccaaccgc ggaaaccgga tcttccgcca gctccgcgat gggccgggcg gtcacttcct
     2761 gaatccggct gcggatcgcc gccttgtcga agccgtcgcg cagcagcgta tcgatgtgtt
     2821 ccgggcagag caccaccagc acctcggtgg cgtagtgggc tttggggtga aaggcggcct
     2881 ccagacaaag gcccaggctg cccgcaagct gagccgcgct gcggctgtcc tgatcgacca
     2941 tgagcgtggg accgctggtc attgtgaaca ccgtcaccac ggagtcttcg ggagcgaatc
     3001 cccgctccac gtgaagcggc gaccagggat tgcgctcctc ccattcggcg aagcacatgg
     3061 tgaacttcat ggggttgccg agcgtcgacc gttccgtgcc tccgggcctc gctccgccga
     3121 tgttgcggat agccaggcgc accgcccggc cgatggtggc gttggcccgc gagccctgcc
     3181 ccagggcacc gagccccatg ttcattccca ggcggtgccg gacgggcccg ttgaccacca
     3241 tcacagggct cgcacccatg gtcgtggcca tgacgccgtg aatgttgtac tcgtcggtgc
     3301 agacggcctc catggctgcc agcaccacgg gcatgtactc ggggcggcag cccgccatga
     3361 ccgcgttgat ggccaccttt tccaccgtag cctcgccgag gttgggcgcc accacaccca
     3421 ccacctcctg cggatcacgg gaggtgccgg aaagcattcg aacgactcgc tccggcgtcg
     3481 gtggtatcag gggcaggcca tcgctgaacc cctggtcgaa catgaattcg aactcgtcat
     3541 cctggcttgc gatctctatg cgccgcgctc tcaaggggcc gttctccgcc tcggcgcgaa
     3601 gacgatcggc aatcaccggg tccaccgaaa gcgaaccgca gcccggcctc cattccggca
     3661 gcgcatccca gtcgatgctc ggcgccgagg ccccggcccg atcggcaagc agctcggcga
     3721 gttcccgcca ctcgtcccgc acgaaaccca ccagcgtgcg ctcgggaacc ccctgggcgt
     3781 cacacaggaa gagcgtgggc accgtatcga tatcgaaggc aaaagagact ttgagggtcg
     3841 agtcatcgag gatgggaaag ccgggcccgt gctgctcctt caatcggcgg ttgccctctc
     3901 gggtctgacc tgccacgagc atgtcgagcg cgccgtccac cgaccggttg agcgcgtcca
     3961 gcacgggcat cacctcgttg caggtcgggc agtcctcttt gacgaagcag accagagcac
     4021 cccgcgaccc gggaaacgtg tgggcattgc cctcttcgtc ctgcagcaga aaggcgggaa
     4081 gcgcgctcat tgctcgctct tggcgtgatc ggagccgaga agttaccgca gatcgatcat
     4141 tcgagaaagc cgttcgccgc gttcttgcgc caccgcccgg cagacggcac catcggatca
     4201 ccgaaaagga ggacgacatc cgtgctgctg agatcattgc gcgttgcgcg ccaggcgttt
     4261 gaggaaggca ccggctggaa gctaacgtca gagggggcct gcaaagggga ggtctgcata
     4321 cccctgtcga aaccggaggg caccgttgtc gatgtggccg agctcgccga gaccctgaac
     4381 atgcccctgg tgggggaacc ggagcggaat ctgtgggcgc tcggacctga ggcgatcgga
     4441 ggccgagcgc tgaccacggc gctggcaccc gagctgaccc tgcccgacct cgacggcaac
     4501 gagttcacgc tgtcgtcgct gcgtggccag aaaatcctcg tctacgcctg ggccccctac
     4561 tgaggctgct ctgcggacct gcccgtgtgg cgggtactgc gcgatgagct gcatccgcaa
     4621 ggtctggagc tcgtgaccgt agggctggac accctgggcg gcgagggttg ccgggccttc
     4681 atcgaagctg ccgaaccaac ccacccggca ctcatcgacc ggcatcatcg gctggccgat
     4741 ctgttcggcg tcatcaacat tccgagcagc atctggatcg acgaggaagg ctggatcgtc
     4801 cggcctgccg agacggcccc cgcgccgccg accccggggg aagcggcttc gcgagaggta
     4861 cccggcgaaa tgccgccacg ctttgtcgag atgatggggg aagccgtgaa aattcagtct
     4921 gatccggtcg cgtaccatgc cgcgctgcgc gactgggtcg agaaaggcag ggagagcgac
     4981 tacgccctgc ctgccgagga ggtggtcgcg cggtcacgta cccgcaatcc cgagattgcc
     5041 cgcgggcacg cccacttcga gctggccacc caactcgaaa tcgacggcca gcacgacgcc
     5101 gcgataaggc acttccggga ggcccaccgg ctggtgcccg attcctggac ctttcgacgc
     5161 caggcctggt ctctggagcc cgtgggcgaa gggcctctgg caagattctg gcaggggccc
     5221 gatcccgagg atccccaggc gtggccctac gagggcgact ggcttaccga catccgagcg
     5281 acgggtgccc agaactacaa cgatccgtgg cgaccatgac gccccaccct ctcccactgg
     5341 agcaagcaac atgtcggaac tgagcgagtt tcggagcgac gtcgctgcct ggctgaacga
     5401 aaactgtcca cccggcgctc gcgggcgcgg gcagatcccc acgggcagca gcaaggtgga
     5461 gctcgaaccg gacgtgaagc tgtggctgga gcgctgcgcc gagcgcggct ggacggcgcc
     5521 gacctggccg accgagtacg gcggcggggg ccactctgcg ggcgagacga agatcatcta
     5581 cgaggaaatg gccgagatcg gtgcgcgggc accgctgatg ggcatggggc tgtccatgat
     5641 cgggccgacg ctgctggagt tcggaacccc ggatcagaaa gaaagacacg ttccatcgat
     5701 cacgcgcggc gaggtgcact ggtgtcaggg gtactctgag ccgaatgccg gctccgacct
     5761 ggccgccctg cagacccggg cagaagaccg aggggatcat ttcgtgatca acgggcagaa
     5821 gatctggact tccggagcaa cgctggccga ctggatgttt gccctggtgc gcaccgactt
     5881 cgatgcccct aagcacgaag gtatcagctt cgttctcctg accatggatc agcccggcgt
     5941 gaccataaag cccatccgcc tgatctccgg gaactcgcca ttctgcgaga ccttcttcga
     6001 cgacgccgtg gccagcaagg cggatctggt cggcgaactg aacaaaggct ggtcggtggg
     6061 caaaaggctg ctccagttcg agcgatccgg catcggcggc cttggcggcg ccaatgcccg
     6121 ggccaacccc ggcagcggcc tggcagagat ggctcgacgt ttcgccggcg gcgcgcccgg
     6181 caggatccag gacccggatg tgcgccaggc tgtcacccgt ttcaacatga accgggccgc
     6241 gttccggctc accacccagc gcgcctcgga ggagaacaga tccggcacgc ccggcgcggc
     6301 tacatccatc ttcaagctgt acggggccgc gctgcagcag gacgcggcgg agctgcgccg
     6361 cgtgttcctc ggcacccagg gtctgggagc cgacgaggaa gcattctcag acgacgagct
     6421 ggagacgacg cgcgaatggc tggccaccag ggcaacgacg atctacggcg ggaccaacga
     6481 ggtgcaggcc aacatcatcg caaaacgcgt gctggggctc ccggactgac gagcagcccg
     6541 ccgtttgccg gcaatggcgg ctgaagcccg tcacggcgcg tgctctaggc gttgcatcag
     6601 gtagcaaagg caatcctgat gcacgacgta aggatcggtc gtgtacatgg cagggaccac
     6661 cggccgcttc aacaagatgg cgccgtcttg cagagcgaaa taacgctccg tcacgtcctg
     6721 gtgagcgccg tccaggacac gcagcacgtc ctctaggcgg ccttcggagc tgccaaaccg
     6781 aaatcccgcg ggaagctcgt ggaaattgac cccctccacg cctccggtca gaaccagcgg
     6841 cgcggcgccg ccttcgtcag cgaaatgaaa gctcacacct aacgggacgt gaacgcgggc
     6901 ttcgctccga tagaggctga agtcctcggc gtgcccggcc ggcaccgcat ccaggcgcag
     6961 gcagcgcagc agataatcgt aggcgcgaat ctcgcactcc ggatcggaca ccgggccgag
     7021 ctccagcgtg accgccggac accggccggc aaaaaggtgg gtaatgacgg tatccggttc
     7081 ctcgatcagc acggccttgt cgcagaagag ataggccatg cccaggttct ccaccgacag
     7141 atcggagaga acagagtaat gcgggttgtg gccagtgttg ttgtgcagat cgacggcggc
     7201 cagaaaccgg cgctcggcca accggccagc cacgccccgc gccatctcac cctcaacacc
     7261 acctgccccc cgccagacgc gattgaaatc ctgctgaccc ggcaacgccc gcatccccgc
     7321 ccgggcggct tgcacgttgc ccacgaaaat cgacatggac cggggcagtt cggggtggtc
     7381 ggcaagaagc cgacgcagac cgttccagcc gctcggctcg ttgccgtgca acagcacgga
     7441 cacgaacagt gccggctccc gcccgccggg cagatgaatc aggctgggca aaccgagggc
     7501 cccttccagc tcccgggctt cacactccag cagacccggc ggcagcgagt ggaactcgtt
     7561 gaaaggcacc gactagagag accaggtgtg gacgggttcg ccggcatcct gctgctggcg
     7621 gtaagcaccg accatgccgt gcaggtccgg gccgttgagg gaaacccagc gacgctgcca
     7681 ggcggctccg ttctgccccg agtcgacccg agccgccacc acgtccagat aggcttcgat
     7741 ctcctccccc gggatgtcct gtgactgcag gccacggcgc gccaagggga ggaggtcttc
     7801 catgatcagg cttcgaacca gcagctcctc ctcacgtcca tcccgaatcc agcgcactct
     7861 gccgttgagc ccatagcgag cgcaattgta gaagttgtct ctggcaacct cgaacggcag
     7921 ccgcgactcc accggctcat cctgcaggcc aaagccgcga atcaggccga aatagaatgc
     7981 gc
//
LOCUS       DBNHLM010000071         4494 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_908199, whole genome shotgun sequence.
ACCESSION   DBNHLM010000071 DBNHLM010000000
VERSION     DBNHLM010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_908199"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="AC16QF_03355"
     CDS             complement(<1..687)
                     /locus_tag="AC16QF_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR6447617.1"
                     /translation="MPHIVDTLIEERARGLMQHPLLWRLVTGLLYPVLGYRAAVRMAD
                     TVADMGGVEIFEMLSAQLKLDIDLGGTEHIPETGSAVVTANHPTGIADGVAVFDVLRT
                     VRSDITFFANRDAIRFAPGLADVIVPVEWVEDKRTHARNRETVSHMVRAFRSERLIVI
                     FPSGRLARPTLRGLVERGWTPTAVNLAQKYRCPIVPMHIRGRNSWLYYLLYAINTELK
                     DMTLFRELLNK"
     gene            complement(675..1451)
                     /locus_tag="AC16QF_03360"
     CDS             complement(675..1451)
                     /locus_tag="AC16QF_03360"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6447618.1"
                     /translation="MHLDWQIHDGKGPHLLLVHGFLCSRAQWLWNLTVLAEVCRPVTV
                     ELWGHGRSPAPSDPECYAPDRIVEEFESIRTALGAEQWFVLGYSLGAGLTIRYALTHP
                     HRVMGHVFTNSTSGLADAEQIRRWQDEADETAQRIIAAGQAALERIPVHPRHATKLPD
                     PLHQALMADAARLDPTGVANVLRHTNPNVSVRADVHRNTRPALLVCGSRERRFQPHRA
                     FAQATMPCLEIVDIPAGHGVNMEAHEPFNAAVSSFVRCHT"
     gene            1567..3258
                     /locus_tag="AC16QF_03365"
     CDS             1567..3258
                     /locus_tag="AC16QF_03365"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6447619.1"
                     /translation="MAYDILIRNGTVVDGSGGAPYPADVGIRNGRIASIGRIREKART
                     TIDAEGHVVSPGFVDGHTHMDAQVFWDPIGSSSCYHGVTSVVMGNCGFTLAPCRRDEA
                     DLVFRNLERAEDLSRDAMLTGIDWQWETFPQFLDAVDGLPKGINYAGYIGHSALRTYV
                     MGERAFTDPATDGDLAAMCRQVQEAVRAGAIGFSTSRTFNHITADDRPVASRLAEWDE
                     VRAIVNAMGETGKGIFEIAGEAPGRDPARIRDYHERLRDLAVESGVTQTWGMFSVRIA
                     PDFWRPYFDLLDETAAAGGRMYAQVHSRALNVLLSFKTSTPFDKWDVWNDVRSLPLDE
                     QKAKLRDPALRARLVEAANREYQGPRVVGAEARPPNWEWVYPMADMSYDQPSMAELAA
                     ERGVDPVELMIDMSLAHDFDIFFRQPIANENQDDVLEMMKHPRAMVTFSDSGAHVSQI
                     MDSSLQTHLLSYWVREKQAFTLPEAVRRITYDTATMWGLHDRGLVRAGMAADLVIFDP
                     ETIGPRMPTVEHDLPAGARRLKQTADGIMHTIVNGEVLLTRNEYGGATPGRLLRA"
     gene            complement(3306..3382)
                     /locus_tag="AC16QF_03370"
     tRNA            complement(3306..3382)
                     /locus_tag="AC16QF_03370"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3346..3348),aa:Arg,seq:tcg)
     gene            3507..>4494
                     /locus_tag="AC16QF_03375"
     CDS             3507..>4494
                     /locus_tag="AC16QF_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6447620.1"
                     /translation="MNELPLVGVTVLDFGQVYNGPYCGFLLAQAGARVIKVESPRGET
                     LRSRGETTGASYPFAMLNGNKECISLNFRSARGQALLRDLVMHVDVVLENFAPGTLER
                     YGVGARTLRGLNPELIYASSTGYGRSGAHRDYRGMDVTIQAMTGVMSITGEGDGPPLK
                     AGPALADFLGGVHLYGGIVTALYRRSQTGEGAVIDISMQDCVFPSLTTALGAYFVAGG
                     QQGPRTGNRHQGLSVAPYNVYAARDGYVAIICIREGHWRGLTRAMGRPELAEDAQFAD
                     MRARAQNMDAVDALVEGWTRTLDREEIFARAQAEGVICAPVQTLEDVINDPHL"
BASE COUNT          755 a         1425 c         1567 g          747 t
ORIGIN      
        1 tttgttgagc agctcgcgaa acagcgtcat gtccttcagc tcggtgttga tggcgtacag
       61 cagatagtag agccaggagt tcctgccgcg gatgtgcatg gggacgatgg ggcatcggta
      121 cttctgcgcc aggttcaccg ccgtaggcgt ccacccgcgc tccaccaggc ctcggagagt
      181 gggccgggcc aggcgcccgg aaggaaatat gacgatcagc ctctcggacc gaaacgcgcg
      241 gaccatgtgg ctgacggtct cccggttccg tgcgtgcgtt cgcttgtctt ccacccactc
      301 gacgggcacg atcacgtcgg cgaggcccgg cgcgaagcga atggcatccc gattggcgaa
      361 gaacgtgatg tccgacctca ccgtgcgcag cacgtcgaac acggcgacgc cgtcggcgat
      421 gccggtgggg tgatttgctg tgaccaccgc gctgcccgtt tcggggatgt gctcggtgcc
      481 gccaaggtcg atgtcgagct tcagctgcgc gctgagcatc tcgaagatct ccaccccgcc
      541 catgtcggcc accgtatcgg ccatgcggac cgccgcgcga taccccagca cgggatacag
      601 caggccggtc accagccgcc acagcagggg gtgctgcatc agtccccggg cccgttcctc
      661 gatgagcgtg tcgactatgt gtggcatcgg acgaacgagc tgaccgccgc gttgaacggt
      721 tcgtgggctt ccatgttgac gccgtggccg gctggaatgt cgacgatctc gaggcagggc
      781 atggtcgcct gggcgaacgc gcgatggggc tgaaagcgcc gttctctcga gccgcagacg
      841 agcagagcgg gacgggtgtt gcgatgcacg tccgcgcgca cggacacgtt cgggttggtg
      901 tggcgtagaa cgttcgcgac gcccgtcggg tcgagacgtg cggcgtccgc catgagggcc
      961 tggtgcagag gatcagggag cttggtcgcg tgacggggat gtacggggat gcgttccagg
     1021 gcggcctgac ctgcggcgat gatccgctgc gcggtctcgt ccgcctcgtc ctgccagcgc
     1081 cgtatctgct cggcgtccgc caggccggag gtcgagttgg tgaagacgtg tcccatgacc
     1141 cgatggggat gggtcagcgc gtagcggatg gtcaggccgg caccgaggga gtagccgagc
     1201 acgaaccatt gctcggcgcc gagggccgtg cggatggact cgaactcttc cacgatccga
     1261 tccggcgcgt agcactccgg atcggacggt gctggcgatc gaccgtggcc ccagagctcc
     1321 acggtgacgg ggcggcagac ctcggccagg acggtcaggt tccacagcca ttgggcgcgg
     1381 ctgcacagaa agccgtggac cagcagcagg tgcggtccct tgccatcgtg tatctgccag
     1441 tcgagatgca tgtaaacggc tgcccttggg atgacgcaaa ataagacatt aaccgatggc
     1501 ccgccatacg gacggtgtcg ctccgtcgcg gacgccggtg atagactgcc acgcaaggag
     1561 ggcggcatgg cttacgatat tctgatcaga aacggaacgg tggtggatgg ttccggtggg
     1621 gcgccttacc cggccgacgt gggcattcgc aacgggcgta tcgcgtccat cggcaggatc
     1681 cgggaaaagg cccggactac catcgatgcc gaggggcacg tggtcagccc gggattcgtg
     1741 gacggccata cgcacatgga cgcccaggtg ttctgggatc ccatcgggtc cagctcctgc
     1801 tatcacggcg tgacgagcgt ggtcatgggc aactgcggct tcaccctggc accgtgccgg
     1861 cgggacgagg cggacctggt cttccgcaat ctcgaacgtg ccgaagatct ctcccgggac
     1921 gccatgctca cggggatcga ctggcagtgg gagacctttc cgcagtttct cgatgcggtg
     1981 gacggcctgc ccaaggggat caactacgcc ggctacatcg gtcattcggc ccttcggacc
     2041 tacgtcatgg gcgagcgcgc tttcaccgac ccggccaccg acggggatct ggcggccatg
     2101 tgccggcagg tgcaggaagc cgtgcgcgcg ggcgccatcg gcttctcgac gtcgcgcacg
     2161 ttcaaccaca tcaccgccga cgaccgtccc gtggccagcc gtctggcaga gtgggacgag
     2221 gtgcgcgcca tcgtcaacgc catgggcgag acgggcaaag gcatcttcga gatcgccggc
     2281 gaagcgccgg gccgtgatcc ggctcgaatt cgcgactacc acgaacggct tcgcgacctg
     2341 gccgtggagt ccggggtgac ccagacctgg ggcatgttca gcgtgcgcat cgcgcccgat
     2401 ttctggcgac cctatttcga tctcttggac gagaccgcgg ctgcgggagg gcgaatgtac
     2461 gcccaggtgc acagccgggc gctcaacgtg ctgctgtcgt tcaagacctc gacgccgttc
     2521 gacaagtggg acgtctggaa tgacgtccgc tcgctccccc tggacgagca gaaggcgaag
     2581 cttcgcgatc ccgccctgcg cgcccggctg gtcgaggccg ccaatcgcga gtaccagggc
     2641 cctcgtgtgg tgggcgccga agccaggccg ccgaactggg agtgggtcta ccccatggcg
     2701 gacatgagct acgatcagcc cagcatggcc gagctggccg ccgagcgcgg cgtggatccc
     2761 gtggagctca tgatcgacat gtcgctggcg cacgacttcg acatcttctt ccgtcagccc
     2821 atcgccaacg aaaatcagga cgacgtgctg gaaatgatga agcatccgcg ggcgatggtg
     2881 accttctccg attccggcgc ccacgtctct cagatcatgg acagctccct gcagacccac
     2941 ctcttgagct actgggtgcg ggagaagcag gcgttcaccc tgcccgaggc ggtccggagg
     3001 atcacctacg atacggccac catgtggggc ctgcacgatc gcggtctggt tcgggcaggc
     3061 atggcggcgg acctggtgat tttcgacccc gaaaccatcg gcccgcggat gcccaccgtg
     3121 gagcacgacc tgccggcggg cgctcgccgg ctcaagcaga cggccgacgg gatcatgcac
     3181 accatcgtca acggcgaggt gctgctgacc cgcaacgagt acggcggagc gaccccgggc
     3241 aggctgctgc gcgcctgagc tcgcacgggc aggcggccgc tcggggctgc caggaagaag
     3301 ggatctggcg cggctgggag gactcgaacc cccgacacct gggttcgaag cccagtgctc
     3361 tatccagctg agctacagcc gctctggaag agtcggtcag tataaccgta tccacctggg
     3421 cgggcgctgc cttttcccgt gagcagtggt ggctggccag caaaccggct accgttcgtg
     3481 cagagacgat gctggtgggg tgcgcgatga acgagctgcc gctggtcggt gtgaccgtgc
     3541 tggatttcgg ccaggtctac aacggaccgt actgcgggtt cctgcttgcc caggcgggcg
     3601 cgcgggtcat caaggtcgag tcgccgcggg gcgagaccct gcgcagccgg ggcgagacga
     3661 ccggcgcgag ctacccgttc gccatgctca acgggaacaa ggaatgcatc tcgctgaact
     3721 tccgctccgc tcgcggccag gcgctgctgc gggacctggt catgcacgtc gacgtggtgc
     3781 tggagaattt cgcgcccggt acgctggagc gctacggggt gggggccaga accctgcgtg
     3841 gcctcaaccc cgagctcatc tacgcgtcga gcacgggcta cggccggtcg ggcgcgcacc
     3901 gcgactaccg cggcatggac gtcaccattc aggcgatgac gggggtcatg agcatcaccg
     3961 gggagggcga cggacctccc ctgaaggcgg ggcctgccct ggcggacttc ctgggcggcg
     4021 tgcacctcta cggcgggatc gtcaccgcgc tttatcgtcg cagccagacc ggtgagggtg
     4081 cggtcatcga catctccatg caggactgcg tctttccttc cctgaccacc gcgctgggcg
     4141 cctacttcgt ggcgggcggg cagcagggtc cgcgcacggg gaaccggcac cagggcctga
     4201 gcgtcgctcc ctacaacgtt tacgctgccc gggatggcta tgtcgccatc atctgcatac
     4261 gcgagggcca ctggcgtggg ctgacgcggg caatgggtcg cccggagctg gccgaggatg
     4321 cgcagtttgc ggacatgcgt gccagggcgc agaacatgga tgcggtggac gcgctggtgg
     4381 agggctggac ccggaccctc gaccgggaag agatctttgc ccgggcccag gccgaaggcg
     4441 tgatctgcgc gccggttcag acgctggaag acgtgatcaa cgatcctcac ctcc
//
LOCUS       DBNHLM010000072         2905 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_788094, whole genome shotgun sequence.
ACCESSION   DBNHLM010000072 DBNHLM010000000
VERSION     DBNHLM010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_788094"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..943)
                     /locus_tag="AC16QF_03380"
     CDS             complement(95..943)
                     /locus_tag="AC16QF_03380"
                     /inference="COORDINATES: protein motif:HMM:NF018677.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HIR6447621.1"
                     /translation="MPERVYCSEFNGDEPMLGTADVVDVWLMLEYRPAWKARALEESA
                     LAAPTRRWLAAGIDALAAAGLRARPQLVRQPELDRDTVSLLVAFGDRLLEFQGTGYDF
                     LNGVDLAGLARGHLPEGARVVSEPRYFVCTNGQRDLCCARFGLPVYAAMRERLGSRVW
                     QVSHLGGHRFAPNVLVLPQATLYGRVQPADVPAFIERVESGEVSLAHLRGRTEYAKPV
                     QAAAGYCGGGELDLVDARGDDAAATVTFRRGSEEITVAVERSDPVDVLKSCGKASEPA
                     YPYRRR"
     gene            1109..2449
                     /locus_tag="AC16QF_03385"
     CDS             1109..2449
                     /locus_tag="AC16QF_03385"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR6447622.1"
                     /translation="MTPTRALAERIAGTQYADIPAPARTVAKQALLDFIGVTMAGIDE
                     PLARMLRDQVNEEGGHPQAQLIGTDERASTVQAALVNGSAGHAHDYDDVHTAMTGHPT
                     VPVAPAVLALAERLGRSGEEAIAAFCAGVDTECILGHYAGPGHYAHGWHATGTLGSFG
                     AAAAAARIMGLDEDATSRALGIAGTQAAGLKSQFGTMCKPLHAGHAAATGVQSASLAQ
                     RGFDSRRDILEVEQGFMATQASGACEEGFARAMERDAYTQDICFKYHAACYLTHSSIE
                     AVNNLCRRNAFDPNDIESIEIRVDAGHFRVCNILKPRSGLEAKFSLRFTAAMALAGVD
                     TASIEIFTDALTQQPELVAFRDKVRVVAHETPNPDSIVTITTRDGSTHREAVNVAIPE
                     RDLDAQWEKLEHKFHALVDDRVGRAHADELAGLVRTLDSQDDLGDFFSLLRVHR"
     gene            2446..>2905
                     /locus_tag="AC16QF_03390"
     CDS             2446..>2905
                     /locus_tag="AC16QF_03390"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039588.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6447623.1"
                     /translation="MSETNDFRDRAVLLSKGNRYEDMTEGRVFDHHWGRTITDGDNAA
                     FTTQTLSFCPLYFNEPYARSLGHPGTVVNPLLVFNTVFGLTVEDLSEGGGPFLGVDDC
                     EFLADVYVGDTLTARSTVVTARDSKGQPAFGIVTWHTEGFNQHGEKVLAYH"
BASE COUNT          504 a         1037 c          933 g          431 t
ORIGIN      
        1 gccgagccgt tcgaccccgt gggcgatgcg aggatgttga ccatcgcgct gcgtgagttc
       61 atcgccgacg agatgcccgg cggcgaacgg cgattcagcg tctccgatac ggatacgctg
      121 gctccgacgc ctttccgcag ctcttgagca cgtccacggg gtcggatctt tccaccgcca
      181 cggtgatctc ctcggacccg cgccggaacg tcaccgtggc ggcggcatcg tcgccccggg
      241 cgtcgaccag atccagctcc ccgcccccgc aataccccgc ggccgcctgc acgggcttcg
      301 cgtactccgt gcgcccccgc aggtgcgcaa gcgacacctc gccggactcg acgcgctcga
      361 tgaaggcggg cacgtcagca ggctgaacgc ggccgtacag ggtcgcctgg ggcagaacga
      421 gcacgttcgg cgcgaagcgg tgaccgccga gatggctcac ctgccacacc cggctgccca
      481 gccgctcccg catcgcggcg taaacgggaa ggccgaagcg cgcgcagcac agatcccgct
      541 ggccgttggt gcagacgaag tagcgcggtt ccgagaccac gcgagcgcct tcgggcagat
      601 ggccccgcgc cagcccggcg agatccacgc cgttcagaaa gtcgtagcct gtcccctgga
      661 attcgagcaa ccggtcgccg aaggcgacca gcagcgaaac cgtgtctctg tcgagctctg
      721 gttggcggac gagctggggg cgagcccgca gccccgcggc cgccagggca tcgatgccgg
      781 cggccagcca gcgtctcgtc ggcgccgcga gcgcgctctc ctccagggcc ctggctttcc
      841 aggccggccg gtattcgagc atcagccaga cgtccaccac gtccgccgtg ccgagcatgg
      901 gctcgtcgcc attgaactcg ctgcaataga cgcgctcggg catggggtcg cctcgcctcg
      961 ccgggagacg gccattgaag cagaaatcgt tccgagcctg tacccggcgg cgctaaaaca
     1021 tggcctcagg cggctttgca aagccatggc aatcccgcta gcttagggct caacagtctc
     1081 cgtccggagg ctttcgggag gaccacggat gacaccgacc cgcgcgttgg ccgagcggat
     1141 cgccggcact caatacgccg acatcccggc gccggcacgc acggtggcga agcaggcgct
     1201 gctcgacttc atcggcgtga ccatggccgg catcgacgag cccctcgccc gcatgctgcg
     1261 cgatcaggtc aacgaggaag gcgggcaccc ccaggcacag ctcatcggca ccgacgagcg
     1321 ggcgtccacc gtccaggccg ccctggtcaa cggctcagcc ggccacgctc acgactatga
     1381 cgacgtgcat acggccatga ccggacatcc caccgtgccc gtcgcgcccg ccgtgctcgc
     1441 tctcgccgag cggctgggtc ggtccggaga agaggccatc gccgcgttct gcgcgggcgt
     1501 ggataccgag tgcatcctcg gccactacgc cgggcccggt cactacgccc atggctggca
     1561 cgccaccgga accctgggca gcttcggagc cgcggccgcg gcagcccgca tcatgggtct
     1621 ggacgaggat gccaccagcc gcgccctcgg catcgccggc actcaggccg cggggttgaa
     1681 atctcagttc gggaccatgt gcaaaccgct gcacgccggc cacgctgccg ccaccggcgt
     1741 tcaatcagcc agcctggcgc agcggggctt cgacagccga cgggacatcc tcgaggtgga
     1801 gcagggcttc atggcgaccc aggccagcgg cgcctgcgag gagggctttg cccgggccat
     1861 ggagcgggac gcctacaccc aggacatctg cttcaagtac cacgcggcct gctacctgac
     1921 ccactcctcc atcgaggcgg tcaacaatct ctgccggcgc aacgccttcg acccgaacga
     1981 catcgaaagc atcgagatcc gggtggatgc cggtcatttt cgggtgtgca acatcctcaa
     2041 gccccgatcc ggcctggagg ccaagttcag cctgcgcttc acggcggcca tggcccttgc
     2101 cggcgtcgac acggcgagca tcgagatctt caccgacgcg ctgactcagc aacccgagct
     2161 ggtggccttc cgggacaagg tccgcgtggt cgcccacgag acgcccaacc cggacagtat
     2221 cgtcaccatc accacccggg acgggagcac ccaccgagag gccgtcaacg tggcgatccc
     2281 tgagcgagac ctggatgccc aatgggagaa gttagagcac aaattccacg ccctggtgga
     2341 cgaccgcgtc ggccgtgccc atgcggacga gctcgccggc ctggtcagga ccctggactc
     2401 ccaggacgat ctgggcgact tcttcagcct gctgcgggtt catcgatgag cgaaacgaac
     2461 gacttccgcg atcgggccgt gctgcttagc aaaggtaacc gctacgagga catgaccgag
     2521 ggccgggtgt tcgaccacca ctgggggcgc acgatcaccg atggggacaa cgcggcgttt
     2581 accacccaga ccctttcctt ctgcccgctc tacttcaacg agccctatgc ccgatccctc
     2641 ggccacccgg ggaccgtggt caatcccctg ctggtcttca acaccgtttt cggtctcacg
     2701 gtggaggatt tgagcgaggg gggcggtccg ttcctgggcg tggacgactg cgaatttctg
     2761 gcggacgtgt acgtgggcga cacgctcacc gcgcgcagca cggtggtgac cgcgcgcgat
     2821 tcgaagggcc aaccggcttt cggcatcgtc acctggcaca ccgaaggctt caaccagcac
     2881 ggggagaaag tgctggccta tcacc
//
LOCUS       DBNHLM010000073         2770 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_489160, whole genome shotgun sequence.
ACCESSION   DBNHLM010000073 DBNHLM010000000
VERSION     DBNHLM010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_489160"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..1328
                     /locus_tag="AC16QF_03395"
     CDS             246..1328
                     /locus_tag="AC16QF_03395"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family RNA-guided transposase"
                     /protein_id="HIR6447624.1"
                     /translation="MKKDTRDGSGLTVGVDLGDRWSRYCVLDAQGEILEEDTIASTGK
                     AFRKLFGERPAARVVIEVGSHSPWVQRLLQELGHETITANPRYVRLIYGSRNKNDQLD
                     AERLARLARVDTKLLHPIQHRGGTAQADRALLRSRNALVECRTKLVNHARGMVKSIGG
                     RLPTCSTSSFHKRVRQDLPNELKPALTPVLDTIAALTKKIQCLETRIERLASKRYPET
                     GLLRQVSGVGPLTAFSYVLTVEDPQRFKSSRAVGPYLGLRPRQSQSGDQDPELRITKA
                     GDTDLRRLLVGSAHYILGPFGPDTDLRRWGLALAARGKKNAKKRAVVAVARKLAVLLH
                     RLWVTAEVYEPLRSQRSAIAAQAVSA"
     gene            1967..>2770
                     /locus_tag="AC16QF_03400"
     CDS             1967..>2770
                     /locus_tag="AC16QF_03400"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6447625.1"
                     /translation="MIGSKLGHYEITAKLGEGGMGQVYRARDTNLRRDVALKFLPAEF
                     VRDAERIARFEREARALAALQHPNIASVYGFEEKDAQRFLIMELVEGEDLAARIARGP
                     LSQEDALSIAVQISRGLEAAHEQGIVHRDLKPANVRVTSEGVAKILDFGLAKALAPDP
                     SDESQHLSHSPTVTSARTRAGVILGTAAYMSPEQSRGHAVDRRADIWAFGCVLYEMLV
                     GRAPFHGDTVTDLLAEIVKSEPDLDALPPGTPASVRRLVRRCLDKDPSRR"
BASE COUNT          533 a          851 c          920 g          466 t
ORIGIN      
        1 cccgtcgttg cagcagccat gagtattttt tggattcgca tgatgctagt tcctagccac
       61 cttcaagcga gtgcgctacc gacggtacac cggcgaaagc gtcgatggtg ccggaggcag
      121 ggctcgaacc tgcacggcct tgcggccaga tgggatgaga aggtcgacct ccgtcgcggg
      181 gtatccgcat aggatcccca agacaacatg tttcgggctc agcaggccca tggcggaggt
      241 cgaccatgaa gaaggatacc agggatggat cggggctgac ggtgggtgtg gatctgggag
      301 atcgatggag ccggtactgc gtcctggacg cacagggcga gatcctcgag gaggacacga
      361 tcgcctcgac cggcaaggcg tttcgcaagc tgttcggtga gcgacctgcg gcgcgggtcg
      421 tgatcgaagt cgggagccac tcgccgtggg tccagcgtct actgcaggag ctcgggcacg
      481 agacgatcac ggccaatccc aggtacgttc gtttgatcta cggcagcagg aacaagaacg
      541 accagctgga tgcggagcga ctggctcgcc tggcccgggt cgataccaag ctgctgcacc
      601 cgatccaaca tcgtggtggt accgctcagg ccgaccgtgc cctgctccgc agtcgcaatg
      661 ccctggtcga atgtcgcacc aagctggtca atcacgctcg cggcatggtg aagtcgatcg
      721 gcgggcgatt gccgacctgc tcgacgtcgt ccttccacaa gcgcgtacgc caagacctac
      781 ccaacgaatt gaagccggca ttgacgccgg tactcgacac gatcgcggca ctgacgaaga
      841 agatccaatg cctcgagact cgaatcgaac gcttggcatc gaagcggtat ccggaaaccg
      901 ggctgctgcg ccaggtctcg ggagtcggcc cgctgacggc attcagctac gttctgaccg
      961 tggaggatcc gcagcgtttc aagagcagcc gtgccgtggg accgtatctg ggcctgcgcc
     1021 caagacaaag ccagtccggg gatcaagacc ccgagctacg aatcaccaag gccggcgaca
     1081 cggatttgcg ccggctactg gttggctcgg cgcactacat ccttggaccg ttcggacctg
     1141 acaccgatct gcgtcgttgg ggacttgcac tggcggcgcg tgggaagaag aacgcgaaga
     1201 agcgcgctgt cgttgccgtg gcgcgaaagc tggcagtgtt gctgcaccgg ctgtgggtca
     1261 cggccgaggt ctacgaacct ctccgtagtc agcggtccgc gatcgctgca caagcggttt
     1321 cggcatgaag tattgaagac acgaaccagg aaggatcgtt aaggcagcag tgatgcgtcg
     1381 ctcaactctc caacattctg atacgcccag tccggacgac tgcgcaaaat ccgcaggccc
     1441 tcgaggccgg ggctcagatt gcagcgtcct gactaagaac ggaccccaga atgcaccgag
     1501 ctccccgaac agagggctca acaagagtgc gaatggaagg gtggcggaac agggtgctag
     1561 ggcgagcggc ggccaccacg ttggccttga caagaggtta ccttctcatg gaagcgaatt
     1621 atgagggcct cggatggggg cggcgtttca agcctttttg aaacgtcgcc ccattttgtt
     1681 gtccagggtt tccaccagtg tccaggattt ccacgctgtt gcgtagagaa tgcgtagagc
     1741 cgccgagcgc agctcgagca tggtgaagtc tccgaggtct tccagcaatc ctttgcgcag
     1801 ccagcggtgc gctgcgagct acgccgcggc gtcgtcagac aagaggcggc gccgggtgtc
     1861 cggtggcgcc gtgccggact cgtcggaccg acaactggcc ccctcggata caattgcggc
     1921 gaggcgctcg acaaggaaga tgcctccctg gggcggagac ccgaagttga tcggaagcaa
     1981 gctcggacat tacgagatca ccgccaagct gggcgagggc gggatgggcc aggtctaccg
     2041 ggcgcgggac accaacctgc ggcgggacgt ggccctgaag ttcctgccgg ccgaattcgt
     2101 ccgggacgcc gagaggatcg cccgcttcga gcgcgaggct cgcgcgctgg ccgcattgca
     2161 gcatcccaac atcgcctcgg tctacggctt cgaagaaaag gacgcgcagc gcttcctgat
     2221 catggagctg gtcgagggcg aggatctcgc agcccggatc gcccggggcc ccttgagcca
     2281 ggaagacgcg ctctcgatcg ccgtacagat ctcccggggc ctggaagcgg cccacgagca
     2341 gggcatcgtg caccgtgacc tgaagccggc caacgttcgc gtcacgagcg agggggtcgc
     2401 caagatcctc gacttcggcc tggccaaggc cctggccccg gatccgtcgg acgagtcgca
     2461 gcacctctcg cattccccca cggtgacttc ggccagaacc cgtgccgggg tgattctcgg
     2521 cactgcggcc tacatgagcc ccgagcagtc gcgcggacac gcggtcgacc ggcgcgcaga
     2581 catctgggcc ttcggctgcg tgctgtacga gatgctggtg gggcgtgcgc cgttccacgg
     2641 ggacacggtg accgatctgc tggccgagat cgtcaagagc gagccggacc tcgacgccct
     2701 tccgcccggg actccggcga gtgtccggcg tctcgtccgt cgctgtctcg acaaggaccc
     2761 gtcgcgtcga
//
LOCUS       DBNHLM010000074        16471 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_369601, whole genome shotgun sequence.
ACCESSION   DBNHLM010000074 DBNHLM010000000
VERSION     DBNHLM010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 16471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16471
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_369601"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="AC16QF_03405"
     CDS             <1..251
                     /locus_tag="AC16QF_03405"
                     /inference="COORDINATES: protein motif:HMM:NF019032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HIR6447626.1"
                     /translation="RALAVVDDMGFELVNADPAAGIIEATDTTFWFGFKDDVAIRVRS
                     AEAGTVVDVRSVSRVGLSDLGANAKRIGDILAALGAGA"
     gene            complement(259..744)
                     /locus_tag="AC16QF_03410"
     CDS             complement(259..744)
                     /locus_tag="AC16QF_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HIR6447627.1"
                     /translation="MKIGVVSDTHNNLRNVGRIVELFNEYGVERVIHTGDITQAKTLD
                     VFARLTAPLWGVYGNNDQERDSLEQAVAAHGFSFTEPPLHLTWHGRKIVVVHDPLEFD
                     GVLNDGHELALHGHTHLYRYEERASQVIFNPGECAGHMAGFNAVGVVDLSDLSAELLR
                     F"
     gene            complement(835..1980)
                     /locus_tag="AC16QF_03415"
     CDS             complement(835..1980)
                     /locus_tag="AC16QF_03415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012336.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6447628.1"
                     /translation="MTDAYVVGVDMIKFGRFPEKTVPEIGAESALMALDDCGLTIQDM
                     EALYCGNLGQASGMVGQRILQQIGQTGIPVVNVSNACATGATAFREAWASVKAGLYDC
                     VLAVGVEQMGKGLLGGAGGGTGIPKEGLLGSGTMPAVFAEAGMEHTRKYGTTFEQFAK
                     VSVKNHHHSTMNPKAMYQIETPLETVMNAEMISYPNTKLMCSVNVDGSAAAVICSEAK
                     AKELGLMKRAVKVRASVLTSDPWQERDLVMPDVNSCTRKAAAAAYEMAGLGPDDIDLI
                     ELHDCFATAEILHYENLGLCGDGEAGRLIDDGEVALGGRIPVNVSGGLLSKGHPLGAT
                     GIANIYEVCTHLRGEAGQRQVEGARIGLTHVIGLGSACGIHILEKTA"
     gene            complement(2005..2421)
                     /locus_tag="AC16QF_03420"
     CDS             complement(2005..2421)
                     /locus_tag="AC16QF_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6447629.1"
                     /translation="MSDTPLPVVEYLKIPEDGDPYLEGYKCGSCGATFLGERSVCSKC
                     GARDQMSAVTLPNHGTLYSYSIVHRSFPGIDVPYVSAVVDLDDGTAIKGNLINVEPDP
                     DKIEFGMPVDVVFADALGRKDRDGNSYLSFFFQPSS"
     gene            2564..3004
                     /locus_tag="AC16QF_03425"
     CDS             2564..3004
                     /locus_tag="AC16QF_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6447630.1"
                     /translation="MKSVKVADYMSTRLITFNRTDSVVEAMAVLLKKKISGAPVVDDE
                     GNLIGILSEKDLMAVVIQDSYYDEGVGIVGDYMREEVDTVDPDSDIYSLADRFHKEGG
                     RRFPVVKNGRLVGQISRRDVLRAAEEFLMHADGLSERKRRKLLT"
     gene            complement(3001..4461)
                     /gene="gatB"
                     /locus_tag="AC16QF_03430"
     CDS             complement(3001..4461)
                     /gene="gatB"
                     /locus_tag="AC16QF_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR6447631.1"
                     /translation="MGFRIPQGWEAVIGLEVHVQLATRSKIFSGASTRFGAEPNTQAC
                     PVDLGLPGVLPVLNAEAVRMAVTFGLAVGAEIRPYSVFDRKNYFYPDLPKGYQISQLP
                     HPIVGRGAITIEASGEAPRTIGITRAHLEEDAGKSIHDAYAGLTGIDLNRAGTPLLEV
                     VSEPELRTPEEAGAYFRKLHSLVRHLKICDGNLAEGSMRCDANVSVRPAGETALGQRT
                     EIKNVNSFRFLERAIEFEIERQIDVLEAGGAIEQQTLLYDPDSHETRPMRTKEYSEDY
                     RYFPDPDLLPVHVSEAFIESVRSTMPELPDARRARYAAEFQLGDYDAAWLTQDPEVAD
                     YFEAAAASAGDAKLAANWVMGDLSAALNREELGIARSPISPSQLGDLIGRIRDETISS
                     KIAKTVFEGLWAGEGEVDEIIAARGLTQVSDAGEIEAIIAGIVAENPDQVAQFRAGKE
                     KVLGFFVGQVMKATQGKANPKQVNELLRKALAADTG"
     gene            complement(4463..5920)
                     /gene="gatA"
                     /locus_tag="AC16QF_03435"
     CDS             complement(4463..5920)
                     /gene="gatA"
                     /locus_tag="AC16QF_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR6447632.1"
                     /translation="MDPFASIGQHAARLRDGSYSSVELTRFFVDRAREHADLNAFICV
                     DEEGALAAAEAADARLRAGTATALTGIPLAHKDIFCTRGTITSCASKMLENFVAPYDA
                     TVVERLNAAGAVTVGKANMDEFAMGSSNENSRFGPVRNPWDPGRVPGGSSGGSAAAVA
                     AGLVPVASGTDTGGSIRQPSAFCGVSGIKPTYGRVSRYGMVAFASSLDQGGFLGRSAE
                     DLALSLAFCAGADPRDSTCSEHVDEWLDGVAENGVGNLEGGLRIGLPREYFADLSTGS
                     ELLDAVAGFFESIGCSLEEVSLPHTSAAIPAYYIIAGAEASTNLSRYDGVRFGYRCEN
                     PGSLEDLYRRSRSEGFGAEVKRRILTGTYALSVGYYDAYYVKAQRLRRLIQRDFLEAF
                     ETVDMILAPTAPGVAFPLGELTHDPVAMYQQDIFTIPASLAGLPAMSLPCGFHQGLPI
                     GVQLIGPHFAEKTLLQAGAAYQRETDWHTRHPEAG"
     gene            complement(5922..6203)
                     /gene="gatC"
                     /locus_tag="AC16QF_03440"
     CDS             complement(5922..6203)
                     /gene="gatC"
                     /locus_tag="AC16QF_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003120927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR6447633.1"
                     /translation="MASIEIAHLCRLAQLALTESEQAAALADLQRIIAMIDEMQAMDT
                     DGVEPLAHPIESDQRLRPDTVSETVDRDHYQRGAPATRDGLYLVPRVVE"
     gene            6373..7413
                     /locus_tag="AC16QF_03445"
     CDS             6373..7413
                     /locus_tag="AC16QF_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR6447634.1"
                     /translation="MFKNIRGLFSRDLSIDLGTANTLIYVRDQGIVLNEPSVVAIRVQ
                     GNQKSVAAVGLDAKRMLGRTPGNITAIRPLKDGVIADFLVTEKMLKYFIAKVHENSFI
                     RPSPRVLVCVPCKSTEVERRAIRESALSAGARDVRLLEEPMAAAIGAGLPVQEAVGTM
                     VVDIGGGTTEIAIISLNGVVYSDTVRVGGDRFDESIVAYVRRTQGSLIGEATAERIKQ
                     EIGAAYPQKEVREIDVRGRNLAEGVPRSFTLNSNEILEALQEPLSMIVQALKSALEQC
                     PPELASDIAETGLVLTGGGALLRDLDTLLAEETGLPVIVAEDPLTCVARGGGKALELM
                     DKAGNADLLSTE"
     gene            7466..8269
                     /gene="mreC"
                     /locus_tag="AC16QF_03450"
     CDS             7466..8269
                     /gene="mreC"
                     /locus_tag="AC16QF_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR6447635.1"
                     /translation="MLTGLVLVSLILVTLDLTTSFMRGTRQVFGTMLAPLHYVAETPY
                     LVGEELGNVFATRSALLDENQMLKRQVLELSKISLQYRNLLKENENLRELLGSRARLP
                     DEVLIAEIIGIVPTVGAHQVVIDKGSDAGVQIGQAVIDSEGLFGQVVEVDVYSSRVLL
                     ITDRDHAVPVQINRNGIRSIAGGTGRMDQLELENVTITADVVKGDLVETSGLGGRFPE
                     GYPVGEVESVVVDPAASLSRVLVRPSALLDRSRHVLVVFAPETRDEVLP"
     gene            8266..8760
                     /gene="mreD"
                     /locus_tag="AC16QF_03455"
     CDS             8266..8760
                     /gene="mreD"
                     /locus_tag="AC16QF_03455"
                     /inference="COORDINATES: protein motif:HMM:NF016019.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR6447636.1"
                     /translation="MNPAQGGWLILASLVVALVLSIFHLPETWPQWLGWLRPAWVPLF
                     VFFWVMALPHRLGLVSAWIFGIFVDVLFGDPIGLNGAVLAGITYIGWRFYERLRMYSV
                     VQQCGVVFLLILGGDLVRMLVHDLSADRGWSWAVLVPALTSMLVWPFVSLFLQRLRLQ
                     FGVE"
     gene            8835..9407
                     /locus_tag="AC16QF_03460"
     CDS             8835..9407
                     /locus_tag="AC16QF_03460"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007998187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR6447637.1"
                     /translation="MVLASGSPRRAELLRQIGLPFVVRPPDVDESLRPGESPERYVER
                     LAIGKARAVVAEDGPALGADTAVVVDEEILGKPADRASAIDMILRLSGRTHRVLTGVA
                     VTDGRAVRSCVVAADVTFRAITRDEAAAYWETGEPRDKAGGYGIQGIGGIFAQSINGS
                     YSAVVGLPLAETEALLHAIGVNTWELRASV"
     gene            9400..10893
                     /locus_tag="AC16QF_03465"
     CDS             9400..10893
                     /locus_tag="AC16QF_03465"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR6447638.1"
                     /translation="MSEELLINVNDFESRVALVSGSVVEEVQVARAGADSLTGNIYLG
                     RVQRIIPGMQAAFVDCGLERPGFLHVSDIAGPRLMLGEEPDGAVDIRELLHDGQQLMV
                     QVAKDPIASKGARLTTQLTIASRYLVLMPFSDHIGISQRVDEEDERERLRLLVDEVRG
                     GQDAGVGFIVRTAADGVDREPLEADVRVLLHIWDRIMARRAECGCPGIVYEELPLHTR
                     VIRDMATAELECIRIDHPPTLERVRKFVAEFLPELTDKVVLYDEPRPIFERFGVDDEL
                     TRALGSRVELKSGGHLVIEQTEAMTTVDVNTGAYLGKHNLEETVFRTNLEAAQVLPRQ
                     LRLRNLGGIIVVDFIDMEDEEHRRQVLRALEKACEGDPARIRIEGFSSLGLVQMSRKR
                     TRESLVQQLCEPCSHCHGAGVVKTAQSTCIEIFRSIMQDAESGCGAHSQGDYVIRAAD
                     NVVDRLLDEDADRLAAMAARVGRRVRIELEPSYGAGEFDIVLVGDTQ"
     gene            11032..14811
                     /locus_tag="AC16QF_03470"
     CDS             11032..14811
                     /locus_tag="AC16QF_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family phospholipid transporter"
                     /protein_id="HIR6447639.1"
                     /translation="MHRIVLRPFLVVVTLTMLFVALMQGTGRLGFMFLPDLELVANQW
                     LSGQRVRVSGLEGSWRYLNPVVRAARVDLPAGHLREVVVELDWLESLVRNRFVARRVL
                     VGDGAVRLEKTPAGKWRLAGTSGDGEFDASGIVYHADELRFSGVIAFGLAGEEEAQSV
                     QVDYLATNRGGLHRHRLHLDNQDADCAAGCGLHLEYQARDGRWPFWDERIQLSLDGGG
                     FVIPAAVLDSPLELMSIGIDWHLRGDESGGRLHAHARGLRLPGGVELAGLVRADVRGF
                     EDVHDAVVHDLAFGLVAHLDDSEEQVRTQRALRMPAVHVRKDAEVVQFWAQRVDMGGV
                     SEFLERALRGSERARRWLSALKLQAEALNVRGYYRLPTGESGFAATLAGLDVDGYRGA
                     PFVRGAAGELLGHARGLELKLKSEDVLLKFPQTFTDTWQMPYLQGAFQAWFGNGYLGL
                     RGVNLRAETLFSRAAGSFALTRPPGERYEERLSLLIGVDALDTDQAFTFVPYTIDEGL
                     EDWLRGGLKAGLLTRIRFGYHGQQHLKVNELARRIELSARISDGRIAYAPDWPDVHEL
                     DADVVVAGPMVRVTVDKGASEGVRLGGSQVVLRNNASYADVELVSRMETDLALDFVRT
                     TPLAESMGFVAPEWVGDGRLEVLGNLHIPIKLPEDVDASEQLQVDVNVRMLDAGLRMP
                     DFRLALGDLEGDLRYRYPNGLRSSRISGTLFGNDVLIDADSNSQEMILSFAGRATHED
                     VLAVLDMEDPGVVTGSADFLASLHVGVDPDHVTRLEVVSDLQGMALDLPADLRKPAEV
                     ARPVEVAVQFLDERQIISFRHGTAQGWLHVADAPERGAVGFGQSPPVLDGRADYLLLT
                     GDLEGFALEEVVPDEEVGGSGFMPLKLSGLRVGQIRLDEFVIFDAVLDGDITAEGFAI
                     TIDSEAVVGRVSMDGDAPLVLGLDRVVLPAGDGEGDPLSPQMAPELPEAVVEVGEFRV
                     GEADYGRWKFNVHPEPDGVRLADLEAVVRGVTVSSPEGVYWSTVDGESRFKGELAAEN
                     LFDVLPQWGFAPSMETESAGMTGELSWAGSPANVDLDLLVGTASVRAETGSFVDVESG
                     GGAVRMFSLINFDTIAKRMRGDFSDLTGKGLSFDKLKATVSFDRGHLAFVEPLKVKGS
                     GSEFEVAGNVDLVTGELDNEMIVTLPVSKSLPWYAAYIALANPLAGAGILVGERVLRK
                     PLEQFSSAKYEIGGTLDEPQVNLVSVFESEMVEAELESLAGAGDEDTFTELEPQE"
     gene            14821..16263
                     /gene="tldD"
                     /locus_tag="AC16QF_03475"
     CDS             14821..16263
                     /gene="tldD"
                     /locus_tag="AC16QF_03475"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017130658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HIR6447640.1"
                     /translation="MTDLLEEAQGQLWAPAGLEQSDLDRLMGRLSSRSIDVGELFFQH
                     QRSESWALEDGIVKDGSFSVDRGVGVRAISGEKTGFAYAEDIILPALTSATEAARSIA
                     RHGEDRSVPAWQVPRVPARYPSVDPISTLDGSEKVALLRRVDEAARQADPRVKEVNVR
                     LSASHESILVMASDGTLCADVRPLVRLDVSVIVEQDGRRERGSAGAGGRGDLGLVSDS
                     EFTDGLAREAVRTALVNLEAVDAPAGPMQVVLGHGWPGVLLHEAVGHGLEGDFNRKGS
                     SAFANRVGEKVASELCTVVDDGTLDGRRGSLTVDDEGSPSQETVLIENGVLRGYMQDK
                     LNARLMKTSSTGNGRRQSFAHLPMPRMTNTFMKAGEHDPEEIIASVDRGIYAVNFGGG
                     QVDITSGRFVFSATEAYLIEKGRVTHPVRGATLIGNGPEVMTRISMVGNDLELDRGIG
                     VCGKDGQSVPVGVGQPTLKIDEVTVGGTSA"
     gene            complement(16310..>16471)
                     /locus_tag="AC16QF_03480"
     CDS             complement(16310..>16471)
                     /locus_tag="AC16QF_03480"
                     /inference="COORDINATES: protein motif:HMM:NF016626.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dual-action ribosomal maturation protein DarP"
                     /protein_id="HIR6447641.1"
                     /translation="PAALTDYLAEHPHSERQTLRHLIERARTAGNEQQQKTASRALFR
                     FLREDVPTG"
BASE COUNT         2683 a         5099 c         5764 g         2925 t
ORIGIN      
        1 cccgggctct ggccgtggtg gacgacatgg gcttcgagct cgtgaacgcc gatcctgccg
       61 cgggcatcat cgaggcgacg gacaccacct tctggttcgg cttcaaggac gacgtcgcca
      121 tccgcgtgcg atcggccgag gcgggcaccg tggtggacgt tcgcagcgta tcccgggtgg
      181 gattgagcga tctgggcgca aacgccaagc ggatcggaga catcctcgca gccctggggg
      241 ccggggcgta aaccggggct agaagcgcag cagctcggcg ctcagatccg acaggtcaac
      301 cacgcccacg gcgttgaacc cggccatgtg gcccgcgcac tcgccgggat tgaagatgac
      361 ctggctggcg cgctcctcgt agcggtacag gtgggtgtgg ccgtgcagcg ccagctcgtg
      421 cccgtcgttc agaacgccgt cgaactccag ggggtcgtgc accaccacga tcttccggcc
      481 gtgccaggtg agatgtagcg gcggctccgt gaatgagaag ccgtgggccg ccacggcctg
      541 ctcgagggag tcccgttcct gatcgttgtt tccgtagacg ccccacaagg gcgcggtgag
      601 ccgcgcgaag acgtccaggg tcttggcctg ggtgatgtcg ccggtgtgga tcacgcgctc
      661 gacgccgtac tcgttgaaga gctccacgat tcgacccacg ttgcgcaggt tgttgtgggt
      721 gtcgctgacg acgccgattt tcatgggctt ggtggttgga ggcgaagggc cggccttcag
      781 ggcggccccg gcctgacggc cggggcggcc acttgggacg gcgatggttt tggtttaggc
      841 cgtcttctcg aggatgtgaa tgccgcaggc gctgcccagc ccgatcacgt gggtgagccc
      901 gatgcgcgcg ccctcgacct gccgttgtcc ggcctcgcca cgcaggtggg tgcagacctc
      961 gtagatgttg gcgatcccgg tcgcgcccag ggggtggccc ttggagagca gccctccgga
     1021 gacgttcacc gggatgcggc cgccgagcgc cacctcgccg tcgtcgatca gccggcccgc
     1081 ttcaccgtca ccgcaaagac ccagattctc gtagtggagg atctcggcgg tggcgaagca
     1141 gtcgtgcagc tcgatgagat cgatgtcgtc cgggccgagc ccggccatct cgtaggctgc
     1201 cgcggccgcc ttgcgggtgc aggagttgac gtccggcatg accaggtcgc gctcctgcca
     1261 tggatccgag gtgagcacgg aggcgcgaac cttcacggcc cgcttcatca gacccagctc
     1321 cttggccttc gcctcggagc agatcaccgc agcggcggag ccgtccacgt tcaccgagca
     1381 catcagcttg gtgttggggt aggagatcat ctcggcgttc atcaccgtct ccaggggcgt
     1441 ctcgatctgg tacatggcct tcgggttcat ggtggagtgg tgatggttct tcaccgacac
     1501 cttggcgaac tgctcgaagg tggtgccgta cttgcgcgtg tgctccatgc cggcttccgc
     1561 gaacacggcc ggcatggtgc cggagcccaa gaggccctcc ttgggaatgc cggtgccgcc
     1621 gccggccccg cccagcagac ccttgcccat ctgctcgacg cctacggcga gaacgcagtc
     1681 gtaaagcccg gccttgacgg atgcccaggc ttccctgaag gcagtggcgc cggtggcgca
     1741 ggcgttggag acgttgacga cgggtatgcc cgtctggccg atctgctgca ggatgcgctg
     1801 gccgaccatg ccgctggcct gacccaggtt gccgcagtaa agcgcctcca tgtcctggat
     1861 ggtgagcccg caatcgtcta aggccatcag cgcggattcg gcgccgatct ctgggaccgt
     1921 cttctcgggg aagcggccga acttgatcat gtcgacaccg actacgtatg cgtctgtcat
     1981 ggagatcctc ctattcgaat cgggtcagga actgggttga aaaaagaaag acaggtacga
     2041 gttgccgtcc ctgtccttgc gccctagcgc gtcggcgaag acgacgtcca ccggcatgcc
     2101 gaactcgatc ttgtccgggt cgggctcgac gttgatcagg ttgcctttga tcgcggtccc
     2161 gtcgtctaag tcgaccaccg cgctcacgta gggcacgtcg atgcccggga acgagcggtg
     2221 gacgatggag taggaataca gggtgccgtg gttcggcagc gtcacggccg acatctggtc
     2281 acgggccccg cacttggagc acacgctgcg ctcgcccaag aacgtggcgc cgcagctgcc
     2341 gcacttgtaa ccctccaggt agggatcgcc gtcctccgga atcttcagat attccacgac
     2401 gggcagtggt gtgtcgctca aagtgtcccc cataaactct tgtggcccag ccccgggagg
     2461 atactattga cggccccctg gcgtcacctg gcgtgggaag gggttttgca gcaatgtact
     2521 gaagagccgt ttcagcggcc agcggatcaa ctggggagag ccggtgaagt cagtcaaagt
     2581 cgcggattac atgtccacgc gtctcatcac gttcaaccgc accgacagcg tggtggaggc
     2641 catggccgtc ctgctgaaga agaagatttc cggcgcgccg gtggtggacg acgagggcaa
     2701 cctcatcggc atcctgtctg agaaagacct gatggcggtg gtcatccagg acagctacta
     2761 cgacgagggc gtgggcatcg tgggcgacta catgcgcgag gaggtggata ccgtcgaccc
     2821 ggattcggac atctacagcc ttgcggaccg gtttcacaaa gagggcgggc ggcgcttccc
     2881 cgtggtcaag aacggccgcc tggtcggcca gatcagccgc cgggacgtgc tgcgggcggc
     2941 ggaggaattc ctcatgcatg ccgacgggct gagcgagcgg aagcgaagga agcttctgac
     3001 ctagccggtg tcggctgcca gggccttgcg gaggagctcg ttcacctgct tggggttcgc
     3061 cttgccctgg gtcgccttca tgacctgccc gacgaagaaa cccagcacct tctctttgcc
     3121 cgcgcggaac tgggcgacct gatccgggtt ctccgccacg atcccggcga tgatggcctc
     3181 gatctcgccg gcatcgctca cctgggtgag gccgcgggca gcaatgatct cgtccacctc
     3241 gccttctccg gcccacagcc cctcgaacac cgtcttggcg atcttgctgg agatggtctc
     3301 gtcccggatg cggccgatga ggtcgccgag ctgtgaaggc gagatgggcg atcgggcaat
     3361 gccgagctct tcccggttga gcgccgcgct gagatcgccc attacccagt tggcggcgag
     3421 cttggcgtcg ccggcggagg cggcggccgc ctcgaagtag tcggcgactt ccggatcctg
     3481 ggtgagccag gccgcgtcgt agtcgccgag ctgaaactcg gcggcgtagc gggcgcgtcg
     3541 ggcatcgggc agttccggca tggtcgagcg caccgactcg atgaacgctt cgctgacgtg
     3601 cacgggcagc aggtcggggt ccggaaagta gcggtagtcc tcggaatatt ccttggtgcg
     3661 cattggcctg gtctcgtggc tgtccggatc gtagagaagc gtctgctgct cgatggcgcc
     3721 gccggcttcg agaacgtcga tctgccgctc gatctcgaac tcgatggcgc gctcgagaaa
     3781 acggaaagag ttgacgttct tgatctccgt gcgctggccc agagctgtct cgccggcggg
     3841 gcgcaccgag acgttggcgt cgcagcgcat gctgccttcg gccaggttgc cgtcacagat
     3901 tttcaggtgg cgcaccaggc tgtgcagctt tctgaagtat gcgcccgcct cctccggcgt
     3961 gcgcagctcg ggttccgaga ccacctcgag aagcggggtg ccggcccggt tcagatcgat
     4021 gccggtcaaa ccggcgtacg cgtcgtgaat ggacttaccg gcgtcctctt ccaggtgggc
     4081 ccgcgtgatg ccgatggtcc ggggcgcttc gccggaggct tcgatggtga tcgcgccccg
     4141 gccgacgatg ggatgaggca gctggctgat ctgatagccc ttgggaaggt ccgggtagaa
     4201 gtagttcttc cggtcgaaca ccgagtaggg tcggatctcc gccccgacgg cgaggccgaa
     4261 cgtcacggcc atgcgcacgg cttccgcgtt cagaacggga agcaccccgg gcaggcccag
     4321 atccacaggg caggcctggg tgttcggctc cgcgccgaac cgggtcgagg ccccggagaa
     4381 tatcttgctt ctcgtggcca gctggacgtg cacctcgagc ccgatgacgg cctcccaacc
     4441 ctggggaatc cggaagccca tctcagccgg cctccggatg ccgggtgtgc cagtcggtct
     4501 cgcgctgata ggccgcgccc gcctgcagga gggtcttttc cgcgaagtgc ggcccgatga
     4561 gctgcacgcc gatgggaagc ccctggtgaa aaccgcacgg cagggacatg gccggcaggc
     4621 cggcaaggct cgccggtatc gtgaagatgt cctgctggta catggcgacg ggatcgtggg
     4681 tcagctcgcc gagcgggaag gccacgccgg gggcggtggg cgcgaggatc atgtccacgg
     4741 tctcgaacgc ctcgaggaag tccctctgga tcagccgccg cagccgctga gccttcacgt
     4801 agtaggcgtc gtagtagccc acggaaaggg cataggtgcc ggtgagtatg cgtcgcttca
     4861 cctccgcgcc gaacccctcg gagcgcgagc gcctgtatag gtcctccagt gagcccggat
     4921 tctcgcagcg atagccgaaa cgcacgccgt cgtagcgcga caggttggtg gacgcctccg
     4981 cgccggcaat gatgtagtag gcgggaatcg ccgcgctggt atggggcagc gagacttcct
     5041 cgagcgagca gccgatgctt tcgaagaagc cggccacagc gtccagcagc tccgagcccg
     5101 tggaaagatc cgcgaagtat tcccgcggca gcccgatccg cagaccgcct tccagatttc
     5161 cgacgccgtt ctcggcgacc ccgtcgagcc actcgtcaac gtgctcgctg caggtggaat
     5221 cccgcgggtc cgcgccggcg cagaaggcca gggacagggc aagatcctcg gcgcttctgc
     5281 ccaggaaacc tccctggtcc aggctcgacg cgaacgccac catgccgtag cgggacacgc
     5341 gcccgtaggt gggcttgatg ccggaaacgc cgcagaaggc ggacggctga cggatggagc
     5401 cgccggtgtc cgtgccggag gccacgggca cgaggccggc ggcgactgcg gcggccgaac
     5461 cgccggagga tccgcccggt actcggcctg ggtcccaggg attgcgcacc ggcccgaagc
     5521 ggctgttctc gttggacgag cccatggcga actcgtccat gttggccttg ccgacggtga
     5581 ccgccccggc cgcgttcagg cgttccacca ccgtcgcgtc gtagggtgct acgaagttct
     5641 cgagcatctt cgaggcgcag gacgtgatcg tgccccgggt gcagaagatg tccttgtgcg
     5701 ccagggggat gccggtgagg gccgtggccg tgcccgctct cagccgcgca tcggcagctt
     5761 cggccgcggc gagcgcgccc tcctcgtcga cgcagatgaa ggcgttcagg tcggcatgtt
     5821 cccgggcgcg gtcgacaaag aaacgggtga gctcaacgct cgagtagctg ccgtctcgca
     5881 gccgggccgc gtgctgcccg atagaagcaa acgggtccac gttactcgac gactcggggt
     5941 acgaggtaca ggccgtcccg ggtggccggc gcgccgcgct ggtagtgatc gcggtcgacg
     6001 gtctcggaga cggtgtcggg tctcagacgc tgatccgact cgatcggatg ggccaacggc
     6061 tcgacgccgt cggtgtccat ggcctgcatc tcgtctatca tggcgatgat gcgctgcaga
     6121 tcggcgagcg cggcggcctg ttccgactcc gtaagggcca gctgggcaag gcggcagagg
     6181 tgggcgattt cgatgctggc catggcgcga agcgtaatgg aaagcggtca ctgaagccat
     6241 tttccggtgc ccgggagggt gtgccgggca gttcccgttg ccgttgcaaa ttgatagagt
     6301 accgtccgcc gagatccccc ctcttgtcag gggatcaagt gttcagtcaa ttaacccgag
     6361 tccttattcc agatgtttaa aaatattcgc gggctgttct cccgggacct gtccatcgac
     6421 ctgggcaccg ccaacacgct catctacgtc agggatcagg gcatcgtgct gaacgaacct
     6481 tccgtcgtcg ccatccgggt gcagggcaac cagaagagcg tggccgcggt agggctggat
     6541 gcgaagcgca tgctgggacg cacaccgggc aacatcaccg ccatccggcc cctgaaggac
     6601 ggcgtcatcg cagactttct cgtcaccgag aagatgctca agtacttcat tgccaaggtc
     6661 cacgagaact cgttcatccg tcccagtccc cgggtgctgg tctgcgtgcc ctgtaaatcc
     6721 accgaggtcg agcggcgcgc catccgcgaa agcgcgcttt ccgccggcgc gcgggacgtg
     6781 cgtcttctcg aggagcccat ggcggccgcc atcggcgcag gcctgccggt gcaggaggcg
     6841 gtggggacca tggtggtgga catcggcggc ggcacgacgg agatcgccat catctcgctg
     6901 aacggcgtgg tgtattcgga taccgtgcgg gtcggtggag accgcttcga tgaatccatc
     6961 gtggcctacg tgcgccgtac ccagggcagc ctcatcggcg aagccacggc ggagcgcatc
     7021 aagcaggaga tcggggccgc ctacccgcag aaagaggtgc gcgagatcga cgttcgcggc
     7081 cgcaacttag cggaaggggt gccgcgcagc ttcacgctga acagcaacga gatcttggaa
     7141 gcgctgcagg aacccctgtc gatgatcgtg caggctctga agagcgccct ggagcagtgt
     7201 ccgcccgagc ttgcctcgga catcgcggaa accggcctgg tgctgacggg cggcggcgcg
     7261 ctgctccgtg accttgacac cctcctggca gaggagaccg gcttgccggt gatcgtcgcc
     7321 gaggatccgc tgacctgcgt cgcccgcggc ggcggcaagg ccctcgagct catggacaag
     7381 gctggcaacg cggacctgct gtcgaccgaa tagggagact caagccatca aagcgctgtt
     7441 tgttcgagaa tctggcgctg gcttcctgct caccgggctc gtcctcgtat ccctgatact
     7501 ggtcaccctg gacctcacca cctctttcat gcgcggcacc cgccaggtgt tcggcaccat
     7561 gctcgctccc cttcactacg tggcggaaac gccgtacctg gtgggagagg aactcggcaa
     7621 cgtgttcgcg acccgctccg cgctgctcga tgaaaaccag atgctcaagc gtcaggttct
     7681 ggagctttcg aagatctcgc tgcagtaccg gaaccttctc aaggagaacg agaatctcag
     7741 ggagctgctc ggcagccggg ccaggctccc ggacgaggtg ctcattgccg agatcatcgg
     7801 gatcgtgccg acggtaggcg cccatcaggt ggtgatcgac aagggctcgg acgccggcgt
     7861 tcagattggc caggccgtca tcgactccga agggctgttc ggtcaggtgg tggaggtgga
     7921 cgtctattcc agccgggtgc tgctcatcac ggaccgggat cacgcggtgc ccgtgcagat
     7981 caaccgtaac ggcattcgca gcatcgccgg cggcacgggc cggatggacc agctggagct
     8041 ggagaacgtg accatcacgg cggacgtcgt gaagggtgat ctggtggaaa cctcgggact
     8101 cggaggccgt tttcccgagg ggtacccggt gggggaggtg gagtcggtcg tggtggatcc
     8161 ggcggcgagc ctgtcccggg tgctggtgcg gccgtcggcg ctgctggatc gatcgcgaca
     8221 cgttctggtg gtgttcgcgc ccgagacccg tgacgaggtg ttgccgtgaa ccctgctcag
     8281 ggcgggtggc tcatcctcgc aagcctggtg gttgccctgg tgctttccat ctttcatctg
     8341 ccggagacgt ggccgcagtg gctgggctgg ctccggccgg cttgggtacc gctgttcgtc
     8401 ttcttctggg tgatggcgct gccccatcgg ctggggctgg tatccgcgtg gatcttcggg
     8461 atcttcgtcg acgtgctgtt cggggatccc atcggcctca acggcgcggt gctggcgggc
     8521 atcacctaca tcggctggcg cttctacgag cgcctgcgaa tgtactccgt ggttcagcag
     8581 tgcggggtgg tcttcctgct gatcctcggc ggtgatctgg tgcgcatgct ggtgcacgac
     8641 ctttccgccg atcgcggctg gagctgggcc gtgctggtcc ccgcgctcac gagcatgctg
     8701 gtctggccgt tcgtctccct gtttctgcag cgcctgcggc tgcaattcgg cgtcgagtga
     8761 gtccggcgtc ggtgccagcc ggccgttcgg cgagccattc ggcaagccat tcggcgagcc
     8821 attcggcgag ccacatcgtg ctggcttccg ggtcgccgcg ccgcgccgag ctgcttcgtc
     8881 agatcggcct tcccttcgtc gtccgcccgc cggacgtgga cgagtcgctt cgcccgggtg
     8941 agtcccccga gcgatacgtg gagcgcctcg cgatcggcaa ggcgcgggcg gtggtggcag
     9001 aggacgggcc ggccctcggg gccgataccg ccgtggtcgt ggatgaggag atcctcggca
     9061 agccggcgga tcgggcatcg gccatcgaca tgatcctgcg cctctccgga cgcacccatc
     9121 gggtgctcac gggggtggcg gtgaccgacg ggcgggccgt gcggtcctgc gtggtggccg
     9181 ctgacgtgac gtttcgggcc atcacccgcg atgaggctgc cgcgtactgg gagacgggcg
     9241 agccgcgcga caaggcgggc ggttacggta ttcaggggat cgggggtata tttgcgcaaa
     9301 gcatcaacgg cagctacagc gcggtggtcg gcttgcccct ggcggagacc gaagcgctgc
     9361 ttcacgccat tggcgtgaac acgtgggagc tgcgggcaag tgtctgaaga actgctcatc
     9421 aacgtcaacg acttcgagtc ccgggttgcg ctggtgtcgg ggtccgtcgt cgaggaggtg
     9481 caggtcgccc gggccggcgc agacagcctg accggcaaca tctatctggg tcgcgtccag
     9541 cgcatcattc cggggatgca ggccgcgttc gtcgactgtg gcctggaacg ccccggcttc
     9601 ctgcacgtca gcgacatcgc aggcccccgg ctgatgctcg gcgaagagcc ggacggggcg
     9661 gtcgacattc gcgagctgct gcacgacggc cagcagctga tggtccaggt ggcgaaagat
     9721 cccatcgcca gcaagggtgc ccgcctgacc acccagctca ccatcgcatc gcgctatctg
     9781 gtgctcatgc ccttcagcga ccacatcggg atttcccagc gagtcgacga ggaagacgaa
     9841 cgtgagcggt tgcgcttgct cgtcgacgag gtgcgcggcg ggcaagacgc cggcgtgggc
     9901 ttcatcgtgc gcaccgccgc ggatggcgtc gaccgggaac cgctggaagc cgacgtgcgc
     9961 gtcctcctgc atatctggga ccggatcatg gccaggcgag ccgagtgcgg ctgccccggg
    10021 atcgtttacg aggagttgcc gctgcacacc cgggtgattc gcgacatggc gacggcggag
    10081 ctggaatgca tacgaatcga tcacccgccg acgttggagc gggtgcggaa attcgtcgcc
    10141 gagttcctgc cggagttgac ggacaaggta gtgctctacg acgagccgag acccattttc
    10201 gagcgcttcg gcgttgacga cgagctgacc cgagcgctgg gcagccgcgt ggaactcaaa
    10261 tccggcggtc acctggtgat cgagcagacg gaagcgatga cgacggtgga cgtcaacacg
    10321 ggcgcctatc tcggcaagca caacctagag gaaaccgttt tccgcacgaa tctggaagca
    10381 gcccaggtgc tcccgcgaca gcttcgcctg cgcaacctcg gcggcatcat cgtcgtggac
    10441 ttcatcgaca tggaagacga agagcatcgc cgtcaggtgc tgcgcgcgct ggagaaggcc
    10501 tgcgagggcg acccggcgcg gattcgcatc gaggggttct catcgctcgg gctggtgcag
    10561 atgagccgga agcgcaccag ggagagcctg gttcagcagt tgtgcgagcc gtgctcgcac
    10621 tgtcacggcg ccggggtggt gaagacggcc cagagcacgt gcatcgagat tttccgctcg
    10681 atcatgcagg atgcggaaag tgggtgcggc gctcactcgc agggcgacta cgtcatccgg
    10741 gcagcggaca atgttgtaga caggttactg gacgaagacg ccgatcggct ggccgcgatg
    10801 gcggcgcgcg tgggtcgccg ggtgcgtatc gaattggaac caagctacgg cgcgggcgag
    10861 ttcgatatcg tcctcgtcgg cgatacgcag tgaaaatgtt gatctgaccg gacgaacggc
    10921 acggaaattt aaggtgaaat gcggggtcga aaggtagtag gggctgaacc gggccttgag
    10981 gccgattccg ggccgacttc cgtaaactaa tctcacatag gaccccatta cttgcaccga
    11041 atcgtactca ggccgttcct ggtcgtggtc acgctgacga tgctcttcgt cgcgctgatg
    11101 cagggcaccg ggcggttggg gttcatgttc ctgccggatc tcgaactggt cgcgaaccag
    11161 tggttgtccg gccagcgcgt tcgcgtctcg ggtctggaag gttcgtggcg ctacctcaat
    11221 ccggtggttc gagcggcgcg cgtggacctc cccgccggtc atctccggga agtcgtcgtg
    11281 gagctcgact ggttggaaag cctggtccgc aaccgcttcg tggcgcggcg ggtgctcgtc
    11341 ggggatggtg ccgtgcggct cgagaagacg ccggccggga aatggcgcct ggcggggaca
    11401 tcgggggatg gcgagttcga cgcgagcggc atcgtctatc acgccgacga gctgcgcttc
    11461 tccggcgtca tcgcttttgg ccttgccggt gaggaagagg cacagtcggt gcaggtggac
    11521 tacctggcca cgaaccgcgg tggcctgcat cgccatcgac tgcatctcga caaccaggac
    11581 gccgactgcg ccgcgggctg cgggctccac ctggagtacc aggcccgcga cggccgctgg
    11641 ccgttctggg acgagcggat tcagctttcg ttggacggtg gcggctttgt cattccggct
    11701 gccgtgctgg acagccctct ggagttgatg agtatcggaa tcgactggca cctgcgcggt
    11761 gacgaatccg gcgggcggct gcacgcccac gcgcgaggac tgcggctgcc cgggggtgtc
    11821 gagttggccg ggctcgtacg cgcggacgtc agaggcttcg aagacgttca cgacgccgtg
    11881 gtgcacgacc tggcctttgg gctggttgcc catctggacg attccgagga gcaggtgcgc
    11941 acgcagcggg cattgcgcat gcccgccgtg cacgtccgca aagatgcgga agtggttcag
    12001 ttctgggctc aacgggtgga catgggtggc gtatccgagt ttctcgagcg agcgctcagg
    12061 ggttcggagc gcgcgcgccg ctggttgagc gctctcaagc tgcaggccga agcgctcaac
    12121 gtccgaggtt actatcgact gcccacggga gagagcgggt ttgccgccac gctggccggc
    12181 ctggacgttg acggctaccg cggtgccccg ttcgttcgcg gggcggcggg agagcttttg
    12241 ggccacgccc gggggctgga gctgaagctg aagagcgagg acgtgctcct caagtttccc
    12301 cagaccttca cggatacctg gcagatgccc tacctgcagg gagcgtttca ggcctggttc
    12361 ggcaacggtt atctcggcct tcgaggcgtc aacctgcggg ccgagaccct gttcagcagg
    12421 gcggcgggca gctttgccct cacgcggccg cccggcgagc gctacgagga gcggctttct
    12481 cttctcatcg gcgtggacgc tctggacacc gatcaggcgt tcacgttcgt gccgtacacc
    12541 atcgacgagg gtctcgagga ctggctgcgt ggcgggctga aagccggcct gctgacgcgc
    12601 attcggttcg gctatcacgg ccagcagcac ctcaaggtga acgagctggc ccgccggatc
    12661 gagctgtcag cccgcatcag cgatggccgc attgcctacg cgccagactg gccggacgtc
    12721 cacgagctcg acgccgacgt cgtggtcgcc ggtccgatgg tgcgtgtcac cgtcgacaag
    12781 ggcgcaagcg aaggcgtccg gctgggcggc tcccaggtcg tgctgcgcaa caacgcctcc
    12841 tacgcggacg tggagctcgt atctcgaatg gagaccgacc tggcgctgga cttcgtgcga
    12901 accacgcccc ttgcggagtc catgggattt gtggcgccgg agtgggtcgg ggatggccgg
    12961 ctcgaggtgc tcggcaacct gcacattccc atcaagctcc ctgaggacgt ggacgcctcc
    13021 gagcagctgc aggtggacgt caacgtcaga atgttggacg ccgggctccg gatgcccgat
    13081 ttccgtctgg ccctggggga tctggaggga gatctgcggt accggtatcc gaacgggctc
    13141 aggtcctccc ggatcagcgg taccctgttc ggcaacgacg ttctcatcga tgccgacagc
    13201 aattcgcagg agatgatcct cagcttcgct gggcgagcca cccacgagga cgtgctggcc
    13261 gtcctcgaca tggaagatcc tggcgtggtg accggcagcg ccgacttcct ggcctcgctg
    13321 cacgtgggcg tggatccgga tcacgtcacc cggctggagg tcgtatccga tcttcagggc
    13381 atggcgctgg atcttccggc cgatctgcgc aagcccgcgg aggtcgcccg accggttgaa
    13441 gttgccgtgc agtttctgga cgagcggcag atcatcagct tccgccacgg taccgcccag
    13501 ggttggctgc acgtcgcgga cgcgcctgag cgcggcgcgg tgggctttgg tcagtcgccg
    13561 ccggttctgg atggccgagc cgactatctg ctgctcaccg gggatctcga gggtttcgcg
    13621 ctcgaagaag tggtccctga cgaggaggtt ggcggatcgg gtttcatgcc gctcaagctg
    13681 agcggccttc gggtgggcca gatcaggctc gacgagttcg tcatcttcga tgccgtgctc
    13741 gacggcgaca tcaccgcgga aggtttcgcc atcacgatcg acagcgaagc cgtggtcggt
    13801 cgcgtgtcga tggacggcga cgcgccgctc gtgcttggct tagaccgggt ggtcctgccc
    13861 gccggtgacg gcgaggggga tccgctcagc ccgcagatgg ccccggagct tccggaggcg
    13921 gtggtcgaag tcggcgagtt ccgggtgggc gaagcggatt acggccgctg gaaattcaac
    13981 gttcaccccg aaccggacgg cgtccggctt gccgatctcg aggccgtcgt gcggggcgtc
    14041 acggtgtcct ctccggaggg cgtgtactgg tccacggtcg acggcgagtc ccggttcaag
    14101 ggcgagcttg ctgccgagaa cctcttcgac gtgctgcccc agtggggctt tgcgcccagc
    14161 atggaaaccg aatccgccgg gatgacgggc gagctcagct gggccggctc gccggccaac
    14221 gtggatctcg acctgctggt cggaaccgcc tcggttcggg ccgagaccgg cagcttcgtc
    14281 gacgtcgagt cggggggcgg cgcggtgcgc atgttcagcc tcatcaactt cgacaccatc
    14341 gccaagcgca tgcgcggcga cttctcggac ctcaccggca agggcctgag ctttgacaag
    14401 ctcaaggcca ccgtcagttt cgatcgcggc cacctggcat tcgtcgagcc cctcaaggtg
    14461 aagggctctg gatccgagtt cgaggttgcg gggaacgtgg atctcgtcac cggagagctg
    14521 gacaacgaga tgatcgtgac gcttccggtc agcaagagcc tgccgtggta cgcggcctac
    14581 atcgctctcg ccaacccgct ggccggagcg ggcatactgg ttggcgaaag ggtgctgcgc
    14641 aaacccctgg agcagttcag cagtgccaag tacgagatcg gtggtacgct ggacgagccc
    14701 caggtcaatc tcgtcagcgt tttcgaaagc gagatggtcg aggccgaact ggagtcgctg
    14761 gcaggcgccg gcgacgagga cactttcacg gaactggaac cacaggagta atgcggcgtc
    14821 gtgactgatc tgctcgaaga ggctcaaggc cagctctggg cgccggcggg cctggaacag
    14881 tcagacctcg acaggttgat gggccggctc agcagccgct ccatcgatgt gggcgagctg
    14941 ttctttcagc atcagcgctc cgagtcctgg gcgctggaag acggtatcgt caaagacggg
    15001 agtttcagcg tcgaccgtgg cgtgggcgtg cgcgccatca gcggcgagaa gacgggtttt
    15061 gcttacgccg aagacatcat tctgcccgcg ctgacgagcg caacggaagc tgcgcgatcc
    15121 attgcccggc acggagaaga ccggagcgtg cccgcctggc aggtaccgcg cgtgcccgcc
    15181 cgctatccca gcgtcgatcc catctcgacg ctggatggct ccgagaaggt cgcgctgctg
    15241 cggcgcgtcg acgaggcggc ccggcaggca gacccgcgcg tcaaagaggt gaatgttcgt
    15301 ctctcggcga gccatgagtc gatcctggtg atggcctccg acggcacgct ctgtgccgat
    15361 gtcaggccgc tggtgcgctt ggacgtctcg gtgatcgtcg agcaggacgg ccgtcgggag
    15421 cggggctccg ccggagccgg aggccgcggg gacctcggcc tggtcagcga ttctgaattc
    15481 acggacggcc tggcccggga ggcggtgcgt acggcgctgg tgaatctgga agccgtcgac
    15541 gcgccggcag gacccatgca ggtcgtgctc ggccacggct ggcccggggt gctgctgcac
    15601 gaggcggtgg ggcacggtct ggaaggggac ttcaaccgca agggcagctc ggcgtttgcc
    15661 aaccgcgttg gcgagaaagt cgcgagcgag ctgtgcacgg tggtggacga cggaaccctc
    15721 gacgggcgcc gtgggtcgct gaccgtagac gacgagggct cgcccagcca ggaaacggtg
    15781 ctcatcgaga acggcgtgct gcggggctac atgcaggaca agctcaatgc caggctgatg
    15841 aagacatcga gcacgggtaa cgggcgccgc cagtcttttg cgcacctgcc catgccgcgg
    15901 atgaccaata ccttcatgaa ggccggggag cacgatcccg aggagatcat cgcgagcgtc
    15961 gaccgcggca tctatgccgt caactttggt ggcggtcagg tggacatcac ctcgggcagg
    16021 ttcgttttct cagccaccga ggcctatctc atcgagaagg gtcgcgtgac ccatcctgtc
    16081 cgtggtgcca ccctcatcgg caacgggccc gaggtcatga ccaggatatc catggttggt
    16141 aacgacctcg agctcgatcg cggcatcggc gtttgcggca aggacgggca gtcggttccg
    16201 gtaggcgtgg gccagcccac gttgaagatc gacgaggtca ccgtcggcgg aacgagcgcg
    16261 tgaacgcaat ggcgattggg gccgcgcgcg cgtcgcccgc cgagcgtgcc tatccggtgg
    16321 gcacgtcttc ccgcagaaag cggaacaggg ctcgggatgc cgtcttctgc tgctgctcgt
    16381 tgcctgccgt gcgggcccgc tcgatcaggt gccgcagcgt ctggcgctcg gagtgcggat
    16441 gttcggcgag gtagtcggtg agcgcggcgg g
//
LOCUS       DBNHLM010000075         5440 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1327738, whole genome shotgun sequence.
ACCESSION   DBNHLM010000075 DBNHLM010000000
VERSION     DBNHLM010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1327738"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="AC16QF_03485"
     CDS             complement(<1..663)
                     /locus_tag="AC16QF_03485"
                     /inference="COORDINATES: protein motif:HMM:NF039714.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HIR6447642.1"
                     /translation="MIERMVVRLFALLAVLVLGSGCSVQFAYNNLDRLVRWGVNDYVN
                     MNDAQRNLFNEEFARLHYWHRTEHMPLYADFFEAVPTTLSDGVDAQELLALENTMMGW
                     AKEIVETGAPLAVAIMQTMTDEQVAGLPARLEESNREIEKTELGKSVEASQARWREEV
                     EDIFTRFAGRLTPAQQEYLVAQSVRYIPERVLWAEYRRRWQRDLMKLLQARSEPAFAD
                     RFA"
     gene            complement(656..1357)
                     /locus_tag="AC16QF_03490"
     CDS             complement(656..1357)
                     /locus_tag="AC16QF_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase-related protein"
                     /protein_id="HIR6447643.1"
                     /translation="MRIGILQTGAVLEDIQPRFGDYPAMFVDLLSGDAYRRPSFSTYR
                     VREGELPEPAACDAYVITGSRHSVYEDHPWIVELAEFVGNTMAGGQKIVGICFGHQLI
                     AHYFGGRTEPASVGWGVGVHGVEIVSRERWMDPHQGSVSMLSSHKDQVVALPRGARRV
                     ASSAFCPNAAFAIGDVVMTWQGHPEFEKDYAETLMRRREALLGPETLAAGLASLDNEI
                     DRALLARWILNFIYD"
     gene            complement(1375..2751)
                     /locus_tag="AC16QF_03495"
     CDS             complement(1375..2751)
                     /locus_tag="AC16QF_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR6447644.1"
                     /translation="MNDGMELLDQKLARYRERGVSRVKLGLTDVDGVIRGKYVSLSKF
                     ASLMEKGGGFCDCVFGWDVHDQLYDAGTYTGWHTGFPDAQYRLVADSERWLSEEGCPF
                     FLGEFVAGDGGPHPLCPRTLLKTVLGRLGDRGLTLKSGFEYEFFVFAETPHSVRDKGY
                     RNLRPLTPGNFGYSLLRASGEADKFSGLMDFSQALDCDLEGLHCETGPGVWEAALQTA
                     LGEEAADRANIFKTFAKVYFNRLDCMASFMAKWSMDYPGQSGHFHLSLLDADGRNVFF
                     DDGQPSGMSDLQRQAVAGLERYLPEYLALIAPTINSYTRLVKGAWAPTASTWGIDNRT
                     VAIRVINGSESHQHIECRVPGADSNPYLVAAAVCAAALGGIEQALLPGDPVVGNSYDV
                     EDSLREELRFPRDLAAAADRLEASDHARAMLGEAFVDHYVMSRRWECEEYRKNLNSWQ
                     LERYFEII"
     gene            complement(3158..3409)
                     /locus_tag="AC16QF_03500"
     CDS             complement(3158..3409)
                     /locus_tag="AC16QF_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447645.1"
                     /translation="MRVFLIALMACLVTLSFVGHGHAADAAAAAPPLVSTGAEAAHQV
                     AAEEGRVDWHLDTAIFTDISPIDTPEVAIKLPEDQQEEG"
     gene            complement(3429..5345)
                     /locus_tag="AC16QF_03505"
     CDS             complement(3429..5345)
                     /locus_tag="AC16QF_03505"
                     /inference="COORDINATES: protein motif:HMM:NF015803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HIR6447646.1"
                     /translation="MKFSPETVRLRRVAKAYAAGEFSRSEYRATRREVIANFRADASS
                     DDDTQRRDAVTVGSGAAVDPDQPRGRSATWLVMVALTMMVLGLVALEARAGTVIGAVS
                     ERSSDPMSSPRLAVRSLRVRAFQPYPGVLESDLNALIDDALDEIRRRNAEGDHGFTRT
                     ELDEVGRFLSALGAHRDGGALSATDAEDLMALIEQQKARRGISVLELEEIAGRVQAYY
                     RDAGYFLAVAYLPAQQVEHGIVEVGVLPGVLGDVVVTGVDQKELASRFEEVVGEPVTE
                     ESITRRVYLLNQVPGLKAQAAFEPGSEVGETRLNVNVVEQRKWSARVIADNHGDDATG
                     KERLSVVGGWFNPRGVGDVLEVGLLQTVNPTNQTYGFIDYRTPIGGVRQAHVRASTNS
                     FTASDGVELDGSGALFDVGTELALRRSRTSSLTARLGVSHHRFSWATAQDQAATFLSA
                     GFDGHRLWDDRRVAAGFGAVADAGRFGDGTFAGQDATFWRLGLNAYAWRALNVPGLPG
                     EQKLAARLLGQLSGSQLPSSRRLSLGGFGRVRGFERDAFLADRGALLSLELRTPVPLG
                     EMALFADTSYGEGLNDLTPAWGHLTSLGVGWDADLLPRLTSRVSLALPVAAKGSGDMD
                     DKGFRIFWQLQYEH"
BASE COUNT         1039 a         1854 c         1629 g          918 t
ORIGIN      
        1 cgcgaagcga tccgcaaagg ccggttcgga gcgggcctgg agcagcttca tgaggtcacg
       61 ctgccagcgc cgccgatact ccgcccagag aacccgctcg ggaatgtagc gaaccgactg
      121 agccaccaga tactcctgct gcgccggggt caggcgaccg gcaaagcggg tgaagatgtc
      181 ctccacctct tcgcgccagc gggcctggga cgcttcgacg ctcttgccga gctcggtctt
      241 ctcgatctcc cggttgctct cctccagccg tgccgggagg ccggccacct gctcatcggt
      301 catggtctgc atgatggcga ccgcgagggg cgcgcccgtc tcgacgatct ctttcgccca
      361 gcccatcatg gtgttttcca gcgcgagcag ttcctgagca tcgacgccgt ccgaaagcgt
      421 cgtcggcacg gcctcgaaga agtccgcata gagcggcata tgctccgtgc gatgccagta
      481 atgaagccgg gcgaattcct cgttgaacag gttccgctga gcgtcgttca tgttcacgta
      541 atcgttcacg ccccaacgca ccagccggtc gaggttgttg taggcaaact gaacgctgca
      601 gccggaacca agcaccagca ccgcgagcag cgcaaacaga cggacgacca tgcgctcaat
      661 catagatgaa gttcagaatc cagcgcgcca gcagcgcgcg gtcgatctcg ttgtcgagcg
      721 aggccaaacc ggccgcaagg gtctcgggcc ccagaagcgc ttcgcggcgg cgcatcaacg
      781 tttccgcata gtccttctcg aactccgggt ggccctgcca cgtcatgacc acatcgccga
      841 tggcaaacgc cgcgttcggg cagaaggcgc tggaggccac gcggcgcgcg ccgcgcggca
      901 gggccaccac ctgatccttg tgactcgaca gcatgctcac cgatccctgg tgcgggtcca
      961 tccaacgttc cctgctcacg atctccaccc catggacgcc tacaccccaa cccaccgagg
     1021 ccggctcggt gcggccgccg aaataatggg caatgagctg gtggccaaag cagatcccca
     1081 cgatcttttg tccgccggcc atggtgttac cgacgaactc cgccagctcg acgatccagg
     1141 gatgatcctc gtaaacgctg tgtcggctgc ccgtgatgac gtaggcgtcg caggccgccg
     1201 gctcaggaag ctcgccttcc cgcacgcggt aggtcgagaa actcgggcgc cgatacgcat
     1261 ctccgctgag cagatccacg aacatggccg ggtagtcgcc aaaccggggc tgaatatcct
     1321 ccagaacggc gccggtctgc agaatgccga tgcgcatcag gcaatgtttc cccgtcagat
     1381 gatctcgaag tagcgttcca gctgccagct gttgaggttc ttgcggtact cttcgcactc
     1441 ccagcgccgg gacatgacgt agtggtcgac gaaggcctcg ccgagcatcg ccctggcatg
     1501 gtcggaagcc tccagacgat cggccgcggc cgccaggtcg cggggaaacc taagctcctc
     1561 gcgcagagag tcctccacgt cgtaggaatt tccgaccacg ggatctccgg gcaggagggc
     1621 ctgctcgata ccccccagcg ccgcggcgca cacggcggca gcgacgagat aggggttaga
     1681 gtcggcgccc gggacacggc actcgatgtg ctggtgcgat tcagatccat tgatgacgcg
     1741 aatggccacg gtgcggttat cgatgcccca ggtcgaagcg gtgggcgccc aggctccttt
     1801 cacgagtcgg gtgtagctgt ttatggtggg agcgatcaac gcgaggtact ccggcagata
     1861 ccgctctaag ccggccaccg cctgacgctg cagatctgac atgcccgacg gctggccgtc
     1921 gtcgaagaaa acgtttcgcc cgtcggcatc gagcaagctc aaatgaaagt gaccgctctg
     1981 gcccggatag tccatggacc acttcgccat gaagctcgcc atgcagtcca gccggttgaa
     2041 gtagactttc gcaaaggtct tgaagatatt ggcacgatcg gccgcttcct cgccgagcgc
     2101 ggtctgcaat gccgcttccc acaccccggg acccgtttcg cagtgaaggc cttccagatc
     2161 gcagtccagc gcctgggaaa agtccatgag accggagaac ttgtcggctt cgccgctggc
     2221 cctcaacagg gagtaaccga agttgcccgg ggtcagcggg cgcaggttcc gatatccctt
     2281 gtcccgcacc gaatgcggcg tctcggcaaa gacgaaaaac tcgtactcga agccagactt
     2341 gagggtgagc ccacggtcac cgagcctgcc cagcaccgtc ttgagaagcg ttcgcgggca
     2401 caggggatgg ggaccaccgt caccggccac gaactcgccc aggaaaaacg ggcacccctc
     2461 ctccgagagc cagcgctcgc tgtcggcaac cagccgatac tgggcatcag gaaaacccgt
     2521 atgccacccc gtataggtac cggcgtcgta gagctggtcg tgcacgtccc atccgaagac
     2581 gcagtcacag aagcccccac ccttctccat cagcgaggcg aacttggaca aactgacgta
     2641 cttcccgcgg atcacgccat cgacgtcggt cagccccagc ttgacacggc tgacaccccg
     2701 ttcacgatac cgcgcgagct tctgatccag cagttccata ccgtcgttca agtaagatct
     2761 cctcgtttgg cctcaacatg tggagcagac tttacctcag gcgaagattt ccacgaattc
     2821 tgtggataac tctgaggata agtcgggcac aagttggcag agccggcgtt ccacggcaac
     2881 caaagaatat tggtcacttt ttgttcaaat tcaaaaaaga tcatcaggat cagatggtta
     2941 gatccccaaa ctaaccgacc atatgaaaaa tgctcgccat ctccccatcc caagttgcca
     3001 ttcttcgcca gtgtgcataa cacggccgtc gcgcttgtta gtgttcgcta acaagccgtg
     3061 ctccgcggca cttaatccgg cagcggaaac gcccgcccca gatgccgctc agtacaaccg
     3121 ttagaccgca gtggccgccg ccggcacgat ccccgggtca gccctcttcc tgctggtcct
     3181 cgggcagctt gatcgccact tctggggtgt caatcggcga gatgtcggtg aagatcgcgg
     3241 tgtccaggtg ccagtccacg cgaccctcct cggcggccac ctggtgtgct gcttcggcac
     3301 cggtgctcac cagcggtggc gcggcagcag cggcatccgc cgcgtggccg tgaccgacga
     3361 agctcaaggt caccagacac gccatcaggg caatgaggaa tacacgcata tccaagtctt
     3421 ctttagggtc aatgttcata ttgcagttgc cagaaaattc gaaatccttt gtcgtccata
     3481 tcaccggagc cctttgcggc caccggcaac gccaggctga cgcggctcgt cagccgaggc
     3541 agcagatccg catcccaacc cacgcccagg ctggtcaggt gaccccaggc gggcgtgaga
     3601 tcgttcaacc cctcgccgta cgacgtatcg gcaaagagcg ccatttcgcc cagagggacc
     3661 ggcgtgcgca gctccaggga aagcagggca ccgcgatcgg cgaggaacgc gtccctctcg
     3721 aagccccgca cgcggccaaa gccaccgagc gacaagcgcc tggagctcgg cagctgggat
     3781 ccgctgagct gtccgagcag cctggccgcc agcttctgct ccccgggaag accgggaacg
     3841 ttgagcgccc gccacgcata cgcgttcaat cccagccgcc agaaggtcgc gtcctgaccg
     3901 gcgaaggtcc cgtccccgaa ccgaccggcg tccgcgacgg cgccgaagcc ggcagcaacg
     3961 cgtcggtcgt cccacaaccg atggccgtca aagccggcgc tgaggaaggt ggccgcctga
     4021 tcctgcgcgg tggcccagct gaagcggtgg tggctgactc ccagccgtgc cgtgaggctc
     4081 gacgtacggc tgcggcgcag ggccagctcc gtgccgacgt cgaagagcgc cccgctgccg
     4141 tccagctcga ctccgtcgct tgcggtaaag ctgttcgtgg aggcgcgcac gtgggcctga
     4201 cggacaccgc cgatgggcgt ccggtagtcg atgaacccgt aagtctggtt cgtcgggttg
     4261 acggtctgca gcagacccac ttccagaacg tcgccgacgc cccgcgggtt gaaccagccg
     4321 cccaccacgc tcagcctctc cttgccggtg gcgtcgtcgc cgtgattgtc ggcaatcacg
     4381 cgagccgacc atttgcgttg ctccaccacg ttgacgttca aacgggtctc accgacttcg
     4441 ctgccgggct cgaacgcggc ctgggcctta agccccggaa cctgattgag caggtagacg
     4501 cgccgcgtga tggattcttc cgtgaccggc tcccccacga cttcctcgaa tcgtgaggcg
     4561 agctccttct ggtcaacccc ggtcaccacc acgtcgccaa gaacgccagg cagcacgccg
     4621 acctccacga tcccgtgctc cacctgctgc gcaggcaggt aggcaacggc gagaaagtac
     4681 ccggcgtccc ggtagtaggc ctgcacccgg ccggcaatct cctcgagctc cagcaccgag
     4741 atgccacggc gggccttctg ctgctcgatg agggccatca gatcttcggc gtccgtggcc
     4801 gaaagagccc cgccatcgcg gtgggcgccg agcgcgctga gaaatcgccc cacctcgtcc
     4861 agctcggttc gggtgaaacc gtggtcaccc tccgcgttgc gccgacgaat ctcgtcgagc
     4921 gcgtcgtcga tcagcgcgtt cagatcgctt tccagcacgc cgggatacgg ctgaaaggca
     4981 cgaacccgca gggaacggac tgccagcctc gggctgctca tgggatcaga gctgcgctcg
     5041 ctcacggccc cgatgaccgt gccggcccgg gcctcgaggg caaccaagcc gagaaccatc
     5101 atcgtcaacg cgaccatcac cagccaggtc gccgaacgcc cgcgcggttg atccggatcc
     5161 acagccgccc cggagccgac ggtcacggcg tcacggcgct gggtgtcgtc gtccgaactc
     5221 gcatctgccc ggaagttggc gatcacctcg cgccgggtcg cccgatactc cgaacggctg
     5281 aactcgccgg cggcgtatgc ctttgcgacc cgccgcaggc gcacggtttc cgggctgaac
     5341 ttcatcagcg tcacgagatt tccctcacca gccggaatcc ggtgaccgca tctgcgcggc
     5401 cctggtgatc ccgcacggtc gtcggcacgc agtcgccgat
//
LOCUS       DBNHLM010000076         4693 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_968450, whole genome shotgun sequence.
ACCESSION   DBNHLM010000076 DBNHLM010000000
VERSION     DBNHLM010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_968450"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="AC16QF_03510"
     CDS             complement(<1..663)
                     /locus_tag="AC16QF_03510"
                     /inference="COORDINATES: protein motif:HMM:NF019850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR6447647.1"
                     /translation="MDASPPLWTWPELCRALSLEVCEGPGVSGIVIDSRKAGPGDLFI
                     ALTGDPGPRFNPSSRSQRDGHDFIDAAVAAGAVGVLAHDGAARDVPQLQVADTLDGLW
                     ALGAAGRARLRCPVVAITGSSGKTTTKGMLAAALGAFSTEGSLNNHLGVPLSLALAPA
                     NASAAVLEIGTNHPGEIAPLSALVRPHVAVVLNVHPAHAENFRDLDELRIEKLSIYSG
                     LKT"
     gene            complement(671..2296)
                     /locus_tag="AC16QF_03515"
     CDS             complement(671..2296)
                     /locus_tag="AC16QF_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6447648.1"
                     /translation="MANLYENDLDANAANFAALTPLTFLARARDIYPHRTAVIYGERR
                     YTWSEHYERCVRVSDALRRRGVGSGDTVAILAGNIPEMLEAHFAVPMAGAVLNAINVR
                     LDAPTVRFILEHGQAAVLLVDEAFGELASHALEGMASAPLVVGIEDSSLENGRLIGAA
                     TWNELVAEGSSEATWALPGDEWAAIALNYTSGTTGNPKGVVYHHRGAYLNAVSNLLTW
                     NMGDHPTYLWTLPMFHCNGGCFPWTLAVNAGTSVCVRQVRADEIFDAIARYGVSHFCG
                     APVVLNTLLSADDGLKAKVTHQVNVMTAGAAPPAAVIEGMEAMGMSVTHVYGLTETYG
                     PVTLCAWQGERWDDLSPSERAALKARQGVRGHMLEGLMVADPETLVPVPMDGATLGEV
                     MMRGNNVMKGYLKNPDATAQSFEGGWFHSGDLAVWHPDGYIQIKDRSKDIIISGGENI
                     SSIEIEDTLYRHPKVREAAVVAKSDARWGETPCAFVELKPDVAASAEEIIDFCRENMA
                     RFKAPKHVVFGELPKTSTGKVQKFVLRERAEQI"
     gene            2404..4212
                     /gene="sppA"
                     /locus_tag="AC16QF_03520"
     CDS             2404..4212
                     /gene="sppA"
                     /locus_tag="AC16QF_03520"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIR6447649.1"
                     /translation="MSEPGLMSRIGSWLTRARNFTANLIFVLLVVLFVTVILTSGERI
                     RVPEGAALVLNPTGIVVDKETLGDAIAELLAPESTRRETELAGLLTALETSAGDDRIK
                     TLVLDLTELDFVSTAHAATLGEAIRTFSDAGKEVIAYAHYFTQPQYLVASYADAVYMH
                     PDGQILPIGYEVNRLFFSEMLNKLDINVHIFRVGKYKEFVEPYTRSDMSDEAREANQK
                     LVDGLWRHYSETVTANREIEAQSFESYAEQYSPLLKAAAGDMGRLALEFHLVDELLTP
                     DQTRARIAATVGYAREGEFNGIGYRDYLTARDSDPARSGGANVGVITTQGPIVMGSDG
                     RGVIAADSTVDLIRRARMDDSVSALVVRVNSPGGSAFASELIRQELELTQLAGKPVVV
                     SMSSVAASGGYWIASTANRIFAHPTTITGSIGIFAVIPTFEESLARIGVTSDGVGSSA
                     LSGSLDLSSGIAEPMAAVLQASVERGYEQFLNLVARGRDMTPEAVDEIAQGRVWIGST
                     ALELGLVDELGGVGEAVEAAAGLAGLEAYGVKQIEEPLSARAMLLKQLGESVEIDLFP
                     EPPQVFALLERGASLIRLLDDPSHRYALCEACTVSW"
     gene            complement(4220..>4693)
                     /locus_tag="AC16QF_03525"
     CDS             complement(4220..>4693)
                     /locus_tag="AC16QF_03525"
                     /inference="COORDINATES: protein motif:HMM:NF018508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HIR6447650.1"
                     /translation="SEADIASKAEEMIAEVRDGSVPSFVVERVNKVQSLVDMLRDVEW
                     DLAAQERKKVVAALAYFADPHDLIPDNVPVLGYIDDAIMIELVVSELKHEIDSFLDFC
                     KYRKEEKARNRNPNLSREDYLGIKRRELHDRMRRRRRSKTSPVRGGTRTRLRLLG"
BASE COUNT          835 a         1574 c         1457 g          827 t
ORIGIN      
        1 tgtctttaac ccactataaa tgctcagctt ttcaatcctc agctcgtcca gatcccggaa
       61 attttccgcg tgggccgggt gaacgttgag cacgacggca acgtgaggcc gcaccagagc
      121 agaaagcggc gcgatctctc cgggatggtt cgtcccgatc tcgagcacgg ccgcgctggc
      181 attcgcggga gccaaggcaa gggacagcgg cacgcccagg tgattgttga gactgccctc
      241 ggtggagaat gctcccagag cggccgccag catgcccttg gtagtggtct tgccgctgct
      301 gccggtgatg gcgaccacgg ggcatctcag gcgcgctcgt ccagccgcac ccagagccca
      361 caggccgtcc agcgtgtccg ccacctgcag ctgcggcaca tcgcgcgctg cgccgtcatg
      421 ggccaggacg cccaccgcgc cggcggcgac ggccgcatcg atgaagtcgt ggccatcacg
      481 ctgcgagcgg ctgctgggat tgaatcgagg tccgggatcc ccggtgagcg cgatgaaaag
      541 atcccccgga cccgccttgc gcgaatcgat cacgatgccc gacacgccgg gaccttcgca
      601 aacctccagg ctcagggccc tgcacagctc cggccaggtc caaagcgggg gcgatgcgtc
      661 cacgcccacc tcagatctgc tccgcccgtt ctctgagcac gaatttctgg accttgcccg
      721 tggacgtctt gggcagctcg ccaaacacca cgtgcttggg ggccttgaac cgcgccatgt
      781 tctcccggca gaaatcgatg atctcctcgg cgcttgctgc cacatcgggc ttgagctcca
      841 cgaacgcgca cggcgtctcg ccccacctgg cgtcactctt tgccaccacc gccgcctcgc
      901 gcaccttcgg gtggcggtaa agcgtgtcct caatctcgat gctggagatg ttctcgcccc
      961 cggagatgat gatgtccttc gagcggtctt tgatctggat gtagccgtcc gggtgccaga
     1021 cggccaggtc accggagtga aaccaacctc cctcgaagga ttgagcggtg gcatcggggt
     1081 tcttcaggta acccttcatg acgttgttgc cccgcatcat cacctcgccc agggtcgcgc
     1141 cgtccatagg caccggcacc agggtttccg gatctgccac catcagccct tccagcatgt
     1201 gcccgcgcac gccctgccgg gctttcaggg ccgcccgctc ggatggggac aggtcgtccc
     1261 agcgctcgcc ctgccaggca caaagggtga cgggcccgta ggtctcggtg aggccgtaca
     1321 cgtgggtcac cgacatgccc atggcctcca tgccttcgat taccgcggcc ggaggcgccg
     1381 ccccggcggt catgacattg acctggtgcg tgaccttcgc tttgagcccg tcatcggcgc
     1441 tcaacaacgt gttcagcacc accggcgcgc cgcagaagtg cgagaccccg tagcgggcaa
     1501 tggcgtcgaa tatctcatcc gcgcgtacct gtctcacgca gaccgaggtg ccggcattga
     1561 ccgccagggt ccaggggaaa cacccgccgt tgcaatgaaa catcggcagc gtccacagat
     1621 aggtcggatg gtcccccatg ttccacgtga gcaaattgga aacggcgttc aggtatgcgc
     1681 cgcggtggtg atacaccacc cctttgggat tgcccgtggt gccggacgtg tagttgaggg
     1741 cgatggccgc ccactcgtca ccaggcagcg cccaggtcgc ctcagacgaa ccctcggcca
     1801 cgagctcgtt ccacgtcgct gcaccaatga gccggccgtt ctcgaggctc gagtcttcaa
     1861 tgcccaccac caggggcgcg ctcgccatgc cctccagcgc gtgggaagcc agctcgccga
     1921 acgcctcgtc caccagcagc acggcggcct ggccgtgctc gaggatgaag cgcaccgtcg
     1981 gcgcatcgag cctgacgttg atggcattga gcaccgcgcc cgccatggga accgcgaagt
     2041 gcgcctccag catctcggga atgttgcccg ccaggatcgc gaccgtatcg ccggagccca
     2101 cgccacgccg tctcagggcg tctgacaccc gcacgcagcg ctcgtagtgc tcggaccagg
     2161 tgtaacgccg ctcgccgtag atgacggccg tccggtgggg atagatatcg cgtgcccgtg
     2221 ccaggaacgt cagcggggtg agcgccgcaa agttggccgc attggcatcc agatcgtttt
     2281 cgtagagatt ggccatgtgc gtgggcgccg gcgggaaatt ctcacttcag cacagggcac
     2341 cgcctagctc aagcacgtcc agcatgagat gcttgaaaga tctaagcaat ccggagccac
     2401 cccatgagcg aaccgggcct gatgtcccgc atcggcagct ggttgacccg cgcgcgcaac
     2461 ttcactgcca acctgatatt cgtgctcctc gtcgttctct ttgtgacggt gatcctcacc
     2521 tccggagagc gcatccgcgt accggaaggg gccgccctgg tgctcaatcc caccggcatc
     2581 gtcgtggaca aggagacgct gggcgatgcg attgccgagc tgctcgcacc cgaatcgacg
     2641 cgccgggaaa cggagctcgc cggcctgctg accgccctgg aaacaagcgc cggggacgac
     2701 cggatcaaaa ccctggtgct ggatctcacc gagctcgact tcgtctccac ggcccacgcc
     2761 gcgaccctgg gtgaggcgat caggacgttc agcgacgctg gcaaggaagt gatcgcctac
     2821 gcgcactact tcacccagcc ccagtacctg gtcgccagct acgccgatgc ggtttacatg
     2881 catcccgatg gtcagatact cccaattggc tatgaagtaa accgcctgtt tttcagtgag
     2941 atgctgaaca aacttgatat caatgttcat atattccgcg ttggaaagta taaagagttc
     3001 gtggagccgt atacccgcag cgacatgtcc gacgaagccc gcgaagccaa ccagaaactg
     3061 gtggacggtt tgtggcgcca ctactcggaa acggtgaccg ccaaccggga gatcgaggct
     3121 cagagcttcg agagctacgc tgagcaatac agccccctgc tgaaggcggc tgccggtgac
     3181 atgggacgac tggcgctgga gttccatctc gtcgatgagc tcctcactcc ggatcagacc
     3241 cgtgcccgta tcgcggccac cgtcggctat gcccgcgaag gcgagttcaa cggcatcggg
     3301 tatcgggact atctgacggc ccgggacagc gaccccgcca gatccggcgg ggccaacgta
     3361 ggcgtgatca cgacccaggg ccccatcgtg atggggtcgg acggccgcgg cgtcatcgcc
     3421 gccgacagca ccgtggacct gattcgtcgg gccaggatgg acgacagcgt gtctgcgctg
     3481 gttgtccgcg tgaactcgcc cggcggatcg gccttcgcct cagagctcat ccgccaggag
     3541 ctcgagctta cccagcttgc cggcaagccg gtggtggtct ccatgagcag cgtggcggca
     3601 tcgggcggct actggatcgc ctccaccgcc aaccgcatct tcgctcaccc caccaccatt
     3661 accggatcca tcggcatatt cgccgtgatc ccaaccttcg aggaaagcct ggcgcgcatc
     3721 ggcgtgacgt ccgacggcgt gggcagctcg gctctgtcgg gctcgctcga cctatcgagc
     3781 ggcatcgcag aacccatggc cgccgtgctc caggcgagcg tggagcgcgg atacgagcag
     3841 tttctcaatc tggtggccag aggcagagac atgacgccgg aggcggtgga tgaaattgcc
     3901 cagggacggg tatggatcgg cagcacggcg ctggagctcg gtctggtcga cgagctgggc
     3961 ggcgtcggcg aagccgtcga ggccgcggcc ggtctcgccg ggctggaagc ctacggggta
     4021 aagcagatcg aggagccgct gagcgcacga gctatgctgc tcaagcaatt gggtgaaagc
     4081 gtcgagatcg acctgtttcc ggagccgccc caggtgttcg ccctgctcga gcgaggcgct
     4141 tcgctgattc gactactcga cgatccgtcc caccgctacg cgctttgcga ggcctgcacc
     4201 gtttcctggt agccgacgat cagcccagca gccggagccg ggtccgggta ccgccgcgga
     4261 cgggactcgt cttcgagcgc cggcgccggc gcatgcggtc gtgcagctcg cgccgtttga
     4321 tgcccaggta gtcctcgcga ctcagattcg ggttgcggtt ccgcgccttt tcctctttgc
     4381 ggtacttgca gaagtcgagg aacgagtcga tctcgtgctt cagctcgctg accaccagct
     4441 cgatcatgat cgcgtcgtcg atgtagccga gcacgggcac gttgtccgga atcagatcgt
     4501 gggggtcggc gaagtacgcc agcgccgcca ccaccttctt gcgttcctgg gccgccagat
     4561 cccactccac gtcgcgaagc atgtcgacca gggactgcac cttattgaca cgctccacca
     4621 cgaacgacgg gaccgagccg tcccgaacct cggcgatcat ctcctcggcc ttgctcgcaa
     4681 tgtctgcctc gct
//
LOCUS       DBNHLM010000077         4923 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1328106, whole genome shotgun sequence.
ACCESSION   DBNHLM010000077 DBNHLM010000000
VERSION     DBNHLM010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1328106"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1569)
                     /locus_tag="AC16QF_03530"
     CDS             complement(22..1569)
                     /locus_tag="AC16QF_03530"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6447651.1"
                     /translation="MVTAKDRAGAPDGNHVDVLIVGAGISGVGSAYHLQQQCPEKSYV
                     ILEMKDTFGGTWETHKYPGIRSDSDLYTFGYRFKPWIGAPIASAAEILKYMGEVIEEN
                     GIEQHIRYGHRINRCSWSSADNLWTVEATRLSDGAVFTFTCNFLWMCQGYYDHENPFT
                     PDWPGMDRYQGQFIHAQLWDPEIDYTGKRILVIGSGATAATVVPAFAEKAEHVTMLQR
                     SPTYFYCRENRNELADRLREIGIDEPTVHRVVRAQIIHDQNEMTKRCLAEPDAVFEDL
                     KALIRLYAGEDFEFEPHFVPKYRVWQQRLAFCPDGDVFRAAAAGKLTVVTDTIDTFTE
                     KGVRTASGEEIEADIIFAATGFRLSVMGDIPFDVDGRPVDWHETVNYRGMMFTGVPNL
                     LWVFGYFRASWTLRVDMLGDFVCRLLRHMDAIGAKRVDVELREEDADMELLPWIEEEN
                     FNPGYLMRGLDELPRRGAKPEWRHNQDYWTEREQIPAIDLDGEEFVYDGARRSGATVR
                     GKAASAS"
     gene            1802..2296
                     /locus_tag="AC16QF_03535"
     CDS             1802..2296
                     /locus_tag="AC16QF_03535"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6447652.1"
                     /translation="MIEVEHRRDIDLPADTVWEEIRHFDRVLKWVPGGEESTIDVQGE
                     GVGAVRDIRLVTQGYVQHRLVAFDDEARMFSYELTAGKPIGMQDYVVVATVTPIDDDH
                     CTIRWAGRMTADASLNEAETGRALEVALGNMTSGIIARLKGEEPAFARQPNEDWQLTS
                     DRGP"
     gene            2397..4022
                     /locus_tag="AC16QF_03540"
     CDS             2397..4022
                     /locus_tag="AC16QF_03540"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6447653.1"
                     /translation="MTSQNQPDYDVIGIGAGFAGLALIHYMREAGLSVRVFDKASDIG
                     GTWTWNRYPGAASDSECYYYCLTFSKELLQEWQWSVRYPGWEENLSYMHFVADKCDMW
                     PHIQLNTHVASADYQEDTGHWLITTADGDQYTCKYFVSAMGMISEPVIPNVKGMDTFK
                     GPCFHSARWPEEGLDYAGKRVGIIGAGATTVQMLPVMAETAASVTVFQRTPNFIMPAV
                     QKTMTPDWEKEIKEHYDEIIAKCRNHVFGMAFDSPVGRNLADTPPDEVQRIFEEHWPR
                     GSFRFVFETFDDLLTNPESNRIAGEFVISKMKEKVKDPEVAETLTPKGYPFFAKRPPL
                     DHGYYEAYNRDNVKLVDIKDREPIVEITETGIRTTENEYELDIIVLATGFQAYTGAQE
                     ALAIRGRNGQLLRDKWEDVSASIMGVFVADFPNMFLITGPQAPFANLPTSIEQNVLYI
                     VDCIKKMESEGFDVCEPQKQAEDEWVEHTAEIHAQTLMAQGDKVHSWMMGANLEDRKP
                     RVLIYFGGANVYYDKMRESADAGFPEVEFERIA"
     gene            4036..4854
                     /locus_tag="AC16QF_03545"
     CDS             4036..4854
                     /locus_tag="AC16QF_03545"
                     /inference="COORDINATES: protein motif:HMM:NF016658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HIR6447654.1"
                     /translation="MRSPTALVWALILALAAQNLCAAPKAQLWEFWTASDSKSTLQVD
                     HGAWQEFLDRYLDPGADGINRIRYARVTAEDRALLQGYLGVLGRTDPRELHRTEQLAY
                     WINLYNALTVEVVLRHPEKDSILRMGKGLFSMGPWNEKLIELAGEPVTLNDVEHRILR
                     PIWRDHRIHYVLNCASLGCPNLSGDVYTRSSLEDQLREAETAYINHERAVTFNDGGGL
                     RLSSIYKWYRDDFAPDSKGLLSYLADHHQSLADRLRAHDRDVEFDYDWSLNRAD"
BASE COUNT         1018 a         1539 c         1495 g          871 t
ORIGIN      
        1 ggctgctcat gtcctctgct ctcaggaagc ggaggccgcc ttgccgcgga ccgtcgcccc
       61 gctccgacgc gccccgtcgt agacgaactc ctcaccatcg aggtcgatgg ccgggatctg
      121 ttcgcgctcg gtccagtagt cctgattgtg ccgccattcg ggcttggcgc cgcgccgcgg
      181 gagctcatcc agaccccgca tgaggtagcc cggattgaag ttctcctcct cgatccaggg
      241 cagaagctcc atatccgcat cctcttcacg caactcgacg tccacccgct tagcgcctat
      301 ggcatccata tgcctcaaga gcctgcagac gaaatcaccg agcatgtcca cgcgcagggt
      361 ccagctggca cggaagtatc cgaacaccca caacaggttg ggcacgcccg tgaacatcat
      421 cccgcggtag ttgaccgtct cgtgccagtc gacgggccgc ccatcgacgt cgaaaggtat
      481 gtcccccata accgacaggc gaaaacccgt cgcggcaaag atgatgtccg cttcgatctc
      541 ctcacccgag gcggtgcgaa cgcctttctc ggtaaaggtg tcgatggtgt cggtcacgac
      601 ggtgagcttg ccggcagcgg ccgcccggaa cacgtcgcca tccgggcaga aggcaagccg
      661 ctgctgccac acccggtact ttggaacgaa gtgcggctcg aactcgaagt cctctccggc
      721 atacagtcga atcagagctt tgagatcttc gaacaccgca tccggctcgg ccaggcagcg
      781 cttcgtcatc tcgttctgat cgtgaatgat ctgagcacga accacgcgat gcaccgttgg
      841 ctcgtcgatg ccgatctccc gcaggcggtc agcgagctcg ttcctgttct cccggcagta
      901 gaagtacgtc ggcgatcgct gcagcatggt cacgtgctcg gccttctcgg caaaggccgg
      961 gaccacggtc gccgccgtgg cacccgaccc gatcaccaga atccgcttgc ccgtgtaatc
     1021 gatctcggga tcccagagct gggcgtggat gaactgcccc tggtagcggt ccatgccagg
     1081 ccagtcgggc gtgaaaggat tctcgtgatc gtagtagccc tgacacatcc acaggaagtt
     1141 gcaggtgaac gtgaagacgg cgccgtccga aagccgggtt gcctccaccg tccataggtt
     1201 gtccgcgctc gaccagctgc agcggttgat tcgatggccg taacggatgt gttgctcgat
     1261 cccgttttcc tcgatgacct cgcccatgta cttcaggatc tcggcggcgc tcgcgatagg
     1321 cgcgccgatc cagggcttga agcgatatcc gaacgtgtag aggtccgagt cggagcggat
     1381 tcccggatac ttgtgggttt cccaggttcc tccgaaggtg tccttcatct cgagaatcac
     1441 atagcttttc tcggggcact gctgctgcag gtggtacgcc gagccgactc cggatatgcc
     1501 cgccccaacg atcaggacgt ccacatggtt tccgtccggc gcgcccgccc gatctttcgc
     1561 ggtgaccacg gcaatcctcc ttcttccttc gtcggccggc ccgggaacag cagaccgcct
     1621 gagagcctag ctgaacttcg cagcgtgcgc cgtaggtact ttcccggaga ccccgcgcaa
     1681 cccggccaac cggccgttca gcgccgtgtc gaccggaacc gggccgctgg ctccacgaaa
     1741 agcggtcagg cgagaggcgt gataaggttc cgccggagca aagcgtggaa cgggggacag
     1801 cgtgattgaa gtcgagcatc gccgcgacat cgaccttcca gccgacacgg tctgggagga
     1861 aatacgccac ttcgaccgtg tgctgaagtg ggttccaggc ggagaagaaa gtacgattga
     1921 cgtgcagggc gaaggggttg gcgccgttcg cgacatacgc ctggtcactc agggctacgt
     1981 ccagcaccgg ctggtggcct tcgacgacga ggcacggatg ttttcctacg agctgacggc
     2041 cggcaaacca atcggcatgc aggactacgt ggtcgtagcc acggtgaccc cgatcgatga
     2101 cgaccactgc accattcgct gggccggcag gatgacggcc gacgcgagcc tgaacgaggc
     2161 cgaaaccggc cgcgccctgg aggtcgccct gggaaacatg acatccggca tcatcgcgcg
     2221 gctcaagggc gaagagccag ccttcgcccg gcagcccaac gaggattggc aattgacgag
     2281 cgatcgcggg ccttaattcc ggaaggccac ccagccagcc aaaggcgtcg ctgccccgag
     2341 cttggcctac ctgtggattg acgtataagc tatcgatatt gagataggag accgcgatga
     2401 catcgcagaa ccaacccgac tacgacgtga ttggaatcgg cgccggcttc gccgggctgg
     2461 ctctgatcca ctacatgcgc gaggccggct tgtcggttcg ggtgtttgac aaggcatctg
     2521 atatcggcgg cacgtggacc tggaaccgct atccgggcgc tgcgagcgac agcgagtgct
     2581 actactactg ccttaccttc tcgaaagaac tccttcagga gtggcagtgg tcggtacgct
     2641 acccgggctg ggaggaaaat ctcagctaca tgcacttcgt tgcagacaag tgcgacatgt
     2701 ggccgcacat ccagctgaat acgcacgttg ccagcgcgga ctaccaggag gacaccggtc
     2761 actggttgat cacgactgcc gacggagatc agtacacctg caagtacttc gtcagcgcga
     2821 tgggcatgat ttccgagccg gtcataccga acgtcaaagg catggatact ttcaagggcc
     2881 cgtgcttcca ctccgcccgc tggcccgagg aaggtctgga ctacgccggt aagcgggtcg
     2941 gcatcatcgg ggcgggcgcg accaccgttc agatgcttcc ggtgatggcc gagaccgcgg
     3001 ccagcgtgac cgtcttccag cgtactccga atttcatcat gccggccgtt cagaagacca
     3061 tgacgccgga ctgggaaaag gaaatcaaag agcactacga cgagatcatc gccaaatgcc
     3121 gcaaccacgt cttcggcatg gctttcgaca gcccggtagg tcgaaacctg gccgatacgc
     3181 cgccggacga ggtccagaga atattcgagg agcattggcc acgcggcagc ttccggttcg
     3241 ttttcgagac cttcgacgac ctgctcacca acccggaatc caaccgcatc gccggagagt
     3301 tcgtcatcag caagatgaag gaaaaggtga aagatcccga ggttgccgaa acgcttacgc
     3361 cgaaggggta ccccttcttt gccaagcggc caccgctgga tcacggatac tacgaggcgt
     3421 acaaccgcga caacgtcaag ctggtcgata tcaaggatcg ggaacccatc gtcgagatca
     3481 ccgagaccgg gatccgcacc accgaaaacg agtacgaact cgacatcatc gtgctggcaa
     3541 ccgggttcca ggcatatacc ggggcccagg aagcgctggc aattcggggc cgcaacggcc
     3601 agctgctcag agacaagtgg gaagacgtgt ctgcctccat catgggcgtg ttcgtggccg
     3661 atttcccgaa catgttcctg atcaccggac ctcaggcgcc cttcgcaaac ctgcccacct
     3721 ccatcgagca gaacgtcctg tacatcgtcg actgcatcaa aaagatggag agcgaagggt
     3781 tcgatgtctg tgaaccccag aagcaggcgg aggacgagtg ggtcgagcat acggccgaga
     3841 ttcacgcgca gaccctcatg gcacagggcg acaaggtgca ctcatggatg atgggcgcca
     3901 acctggaaga cagaaaaccg cgcgtgctga tctacttcgg cggcgccaac gtgtactacg
     3961 acaagatgcg tgaatccgcc gatgccggat ttccggaagt ggagttcgag cgaatagcct
     4021 gatgcggatc cgggcgtgcg ctcgccaacc gcattagtct gggcgctgat tctggcgctg
     4081 gccgcgcaga acctttgcgc ggccccgaaa gcccagctct gggagttctg gacggcaagc
     4141 gactcgaaga gcacccttca ggtggatcac ggggcctggc aggaatttct cgaccgctat
     4201 ctcgatcctg gggccgacgg tatcaaccgg attcgctatg cgcgggttac cgcagaggac
     4261 cgcgccctgc tgcaaggcta cctcggcgtc ctcggcagaa cggaccccag ggagctccac
     4321 aggacggagc agctggccta ctggatcaat ctgtacaacg cgctcaccgt agaggtggtg
     4381 ctcaggcatc cggagaaaga cagcatcctg cggatgggaa agggactgtt cagcatgggt
     4441 ccctggaacg agaagctgat cgagcttgcc ggggaaccgg taacgctgaa cgacgtagag
     4501 catcgcattc tccgccccat ctggcgcgac caccggatcc actacgtcct gaactgcgca
     4561 agcctgggct gccccaacct gagcggcgac gtctacacgc gctcgagcct ggaggaccag
     4621 ctgcgcgaag cggagacggc ttacatcaac cacgagcgcg cggtgacctt caacgatgga
     4681 ggaggactgc gtctttcgag catctacaag tggtaccggg acgacttcgc ccctgacagc
     4741 aagggcctgc tgagctacct cgcagaccac catcagagtc tggccgatcg tcttcgcgcc
     4801 catgaccggg acgtggagtt cgactacgac tggagtctga atcgggcgga ctgaagtcgg
     4861 tggacgagca ttgcacggca gcgatgcgaa aggccggctc tcctgcccgt tgcgccccta
     4921 tct
//
LOCUS       DBNHLM010000078        12790 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_310599, whole genome shotgun sequence.
ACCESSION   DBNHLM010000078 DBNHLM010000000
VERSION     DBNHLM010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12790)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12790)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12790
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_310599"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..2110)
                     /gene="rpoD"
                     /locus_tag="AC16QF_03550"
     CDS             complement(275..2110)
                     /gene="rpoD"
                     /locus_tag="AC16QF_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIR6447655.1"
                     /translation="MSTQIEQQQSRLKELIAKGKEQGYITYSEVNDHLPDDISDPDQI
                     EDIIRMINDMGITVYETAPDADELLSTEENTADELAAEEAAAALAAVETEAGRTTDPV
                     RMYMREMGTVELLTREGEIAIAKRIEEGIRDMLSAMAYFPGTVEYLLEVYSEANAEGR
                     LSDMLVGYLDPTDNVPQAAQVQPGAQNQKDDDDEEEDKGPDPVEAKRRFTALKRQYNK
                     TYKVIAEKGRNSKEAQAQLEKLGVAFSPFKLVPRHFDELVDMPRMALAIVRRHERIIM
                     NACVRQARMPRAVFRKEYVGSETSVAWVNKHIKAGKDYSKGLDAQKDEIARAQKKLKA
                     LVKETGLTVGEIKDINRRVSLGEAKARRAKKDMVEANLRLVISIAKKYTNRGLQFLDL
                     IQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINK
                     LNRVSRQMLQEMGREPTPEELGDRMDMPEDKVRRVLKIAKEPISMETPIGDDEDSHLG
                     DFIEDSTIGSPVDLATGEGLKEATREVLGGLTAREAKVLRMRFGIDMNTDHTLEEVGK
                     QFDVTRERIRQIEAKALRKLRHPSRSEHLRSFLDEWMESGGGGKR"
     gene            complement(2311..4035)
                     /gene="dnaG"
                     /locus_tag="AC16QF_03555"
     CDS             complement(2311..4035)
                     /gene="dnaG"
                     /locus_tag="AC16QF_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR6447656.1"
                     /translation="MAGRIPQSFINDLLNRVDIVDVIDARLQLRKAGRDYQALCPFHN
                     EKTPSFTVSPEKQFYHCFGCGESGTALTFLMEHDRMEFVEAVEALAQLVGVEVPREQG
                     GRPARDNTDLYDVLAAADRYFRKALRQSPEAIEYLKARGVTGTIARDFGIGYAPDEWQ
                     GAASALAGMGEAKLLEAGLLTRGEKGRAYDRFRGRIMFPIRDTRGRIIGFGGRVMADG
                     GGPKYLNSPETPVFHKGRELYGLYEARQALRRIDRLIVVEGYMDVVALAQAGISNAVA
                     SLGTAATEEHFQSLYRYTGEVICCFDGDAAGRQAAWRALESALPALSDGRGLKFMFLP
                     DGEDPDSLVRARGKAGYLELAARAVPAVEYLFGRLEEGLDLGSVDDKARFASLAMPHI
                     DRLPAGILKELMASRLEGLTGFRPSAQARPSRGPARRTAADVSLTPLARRLVGYLLRE
                     PSVVSALSEEARRALRPLAGNDLLAELVTYLDEHPDAETPELLGRWTGHDLHDELTGL
                     ARQPSAIDIAAVRGDFTEGVARYLEMQQASARRQLISEMHQDPSAERLKDYWSLKQGP
                     RDKVSGTD"
     gene            4140..4382
                     /locus_tag="AC16QF_03560"
     CDS             4140..4382
                     /locus_tag="AC16QF_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447657.1"
                     /translation="MRTTVRLPGELLTKAKEFAHERGTTLTALIEEGLIMVLERAPKA
                     GKMKPVRVPVSKSTGGLQPGVDLSDSAALLDAMEQG"
     gene            4379..4801
                     /locus_tag="AC16QF_03565"
     CDS             4379..4801
                     /locus_tag="AC16QF_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HIR6447658.1"
                     /translation="MKLADVNVLVGAFRTDSSHHERCRSWLKSTVNAPASFGLAAQVL
                     SGVIRVTTHPKVFVNPSPIDESIRFANFLLGRPNAIVVSPGERHWTIFTDLCRSGGIQ
                     GNLVPDAWFAALAIESGCEWVTLNGNFARFEGLSWSAP"
     gene            complement(4808..6232)
                     /locus_tag="AC16QF_03570"
     CDS             complement(4808..6232)
                     /locus_tag="AC16QF_03570"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HIR6447659.1"
                     /translation="MEKVIPVLLAGGTGSRLWPLSRELFPKQFHALLGDDSLLQSTLK
                     RADRTTGEAPIIVCNEEHRFMVAEQCRAIGQSWRRIILEPEGRNTAPAIALAALAACE
                     EAGPDSAPLLLVLPSDHLITDLDGFASAAELAARAAGEGALVTFGVVPTSPETGYGYI
                     EAPGAELGSGVVRVRSFVEKPDLATANEYLAAGDYFWNSGMFLLNARAYLDELGRHSP
                     LMLEKASLAFERGAEDLDFFRPGPEFLDCPADSIDYAVMEKTDRAMVVPAGFDWSDVG
                     SWSAIWEASDRDGNGNRLEGDVISVDTQNSFVSARDRLVGTIGVSDLVVVETSDAVLV
                     ADKDRVQDVRAVVEQLKAAGRDEYVHHREVFRPWGSYESVGAGERYQVKRIKVKPGAS
                     LSLQMHHHRAEHWIVVRGTAEVTRGDETITLAENQSTYIPLGVTHRLANPGKLTLELI
                     EVQVGSYLGEDDIVRFDDIYGREG"
     gene            complement(6347..6814)
                     /locus_tag="AC16QF_03575"
     CDS             complement(6347..6814)
                     /locus_tag="AC16QF_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR6447660.1"
                     /translation="MSDLPISELRQAITAATTAAMKARDKQRLAVLRLVNADIKRAEI
                     DERRAASTDAEVLAVLNRMLKQRQDALEQYTQAGRKDLADQESFEIDLIGEFMPEQLS
                     EADLASAIDQAIAEANAESMRDMGKVMGLLKDRLQGRADMGAVSGRVKALLAG"
     gene            complement(6837..7052)
                     /gene="rpsU"
                     /locus_tag="AC16QF_03580"
     CDS             complement(6837..7052)
                     /gene="rpsU"
                     /locus_tag="AC16QF_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR6447661.1"
                     /translation="MPSVKVKENEPFDVALRRFKRSCEKAGVLSEVRRREYYEKPTSV
                     RKRKAAAAVKRHLKKLQREARRFEKSF"
     gene            7160..8182
                     /gene="tsaD"
                     /locus_tag="AC16QF_03585"
     CDS             7160..8182
                     /gene="tsaD"
                     /locus_tag="AC16QF_03585"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR6447662.1"
                     /translation="MLVLGIETSCDETGVALYDGVEGLLADALHSQVEVHAAYGGVVP
                     ELASRDHIRKLVPLVRKTLDDSGRSLADVDGVAYTSGPGLMGALLVGAGFARSLAWSL
                     GKPAVAVHHMEAHLLAPLMNPDTAPPMPFIALLVSGGHTQLVLVNAIGDYELLGESVD
                     DAAGEAFDKAAKMLDLGYPGGPNLARAAEGGDPHRFTFPRPMTDRPGLDFSFSGLKTY
                     TLNTTRDLAPMSDADRADVARAFEEAVVDTLYIKCRRAVEQTGITHLVMAGGVAANAR
                     LRRKLSEDLRAAVYYPPARLCTDNGAMIAYAGCLRLQAGERAPLAIETRARWPIVELG
                     RPGPPA"
     gene            8186..8539
                     /gene="folB"
                     /locus_tag="AC16QF_03590"
     CDS             8186..8539
                     /gene="folB"
                     /locus_tag="AC16QF_03590"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF014233.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR6447663.1"
                     /translation="MDSVFVRNLTVSTIVGVYPEERTRPQRVIVSFELFADTRAAAQS
                     DDLEDALDYGEAASRVAEFVRGSRFELIEALADGIAALLLEGYPAARVVVEVLKPGAV
                     TDAETVGVKIERSRA"
     gene            complement(8591..9664)
                     /locus_tag="AC16QF_03595"
     CDS             complement(8591..9664)
                     /locus_tag="AC16QF_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA nucleotidyltransferase/poly(A)
                     polymerase-related protein"
                     /protein_id="HIR6447664.1"
                     /translation="MDVYLVGGAVRDELLGRPVRERDWVVVGASPEAMLKRGFRQVGR
                     DFPVFLHPDTSEEYALARTERKTGPGHKGFECHAGPDVTLEQDLERRDLTVNAIARAS
                     DGSLIDPFGGRSDLEAKVLRHVSVAFSEDPLRVFRVARFAAQLDDFTVAEETRALMAE
                     LAGSGALAELSAERVWQELVKALGSKAPLRFFTVLASVGGLSTWFAELAEVEITFPEA
                     IEDALGRFAALGWHLAPEAVDTLCERLRAPRRHRRAMGHIASHGVVLAAWETAEAGSL
                     CAALKSVGAFKAGDDPEMALRVSAACAGRNPEPLLELAASIRQEVTTASVGGSLTGAA
                     LGEAIDQARTQAIRQAQRSNVGR"
     gene            complement(9682..10890)
                     /locus_tag="AC16QF_03600"
     CDS             complement(9682..10890)
                     /locus_tag="AC16QF_03600"
                     /inference="COORDINATES: protein motif:HMM:NF017591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HIR6447665.1"
                     /translation="MDRRSFLTIAGIGAAYLLRPSLARACPTQGGSSSANWADRLGPG
                     SAMGRLGELLAPDHNGIRLPAGFSSRIVAESGNRPVSSSSYAWHAAPDGGATFAHPEG
                     GWVYVSNCEMPLGFGGASALRFDESGTLVDAYPILGRTSGNCAGGATPWGTWLSCEEV
                     RRGRVYECDPLGLRQPVALPALGRFQHEAAVVDPLTGTVYMSEDEPDGRLYRFRPTLP
                     RHLERPDLSAGVLEVASLAPDGSGSVVWLRVPDPEAGRKPTRWQVPESTGFDGGEGMS
                     YCDGVVYLATKGDNRVWALDLGEDRLEVIYDQATAATPILGGVDNVETNASGDVLVAE
                     DGDDMQIVVLPREGEPVALLQVTRQGGSEITGLAFTPDMSRLYFSSQRGFSGRVTGGI
                     TYEVTGPFVG"
     gene            complement(10910..12442)
                     /locus_tag="AC16QF_03605"
     CDS             complement(10910..12442)
                     /locus_tag="AC16QF_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HIR6447666.1"
                     /translation="MEILLPGRGPREPISDGGDWTFELIGRYDREIGAIAEQFGLDTY
                     PNQIEIIRSDQMMDAYASIGMPIGYHHWSFGKHFVDVERSYQRGFMGLAYEIVINSNP
                     CISYLMEENSMTMQALVIAHACYGHNSFFKGNYLFQTWTDASAIVDYLLFARNYIASC
                     EERYGVQEVEMILDSCHALMNYGVDRYKRPYPISAEEEKRRQAERAEYVQRTLNDLWR
                     TLPQARQLDSSDEEVFPREPQENLLYFIEKHAPLLEPWQREIVRIVRKVAQYFYPQRQ
                     TQVMNEGWASFWHYTLLNELYDRGLVTDGFMMEFLASHTAVLFQPSFDHPAYRGINPY
                     ALGFAIYSDLRRVCEHPTDEDREWFPDIAGGNWLEVLPAAMRDFKDESFVLQFLSPRV
                     MREMRLVGVVDDDAEDEVLISAIHDRGGYREVRSLLAGQYNIGDREPNIQVVRVDVRG
                     DRSLTLRHAQHARQPMGQSTDEVMRHLHRLWGFPVRLESVWHDEVVATTACPAEEAQS
                     AA"
     gene            complement(12442..>12790)
                     /locus_tag="AC16QF_03610"
     CDS             complement(12442..>12790)
                     /locus_tag="AC16QF_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HIR6447667.1"
                     /translation="ETGGTVVSSALKLMLEVLEDRYSPNEWNIYGAQASDGDNWNDDS
                     PLCRQLLEKKILPLVQYFSYVEITKRSHQALWREYEHLAERFPESFAQRQITDVSDIF
                     PVFHDLFQKKDAA"
BASE COUNT         2190 a         4295 c         4027 g         2278 t
ORIGIN      
        1 gcttggctca gggttgccat cgccacataa gttcgcgcgt taaggtttcc ctgaattcat
       61 cccgttcaac tctgatctct cgatacagag cgcacctttc gatgagtcca ctgctctaac
      121 caactgagct ataggcccta cggcgaagtt taccactccc cgaaatcgca cgttatcccc
      181 ccccgatcag caaaacggat ccgccccacg aaaaaggccc tctctcgagg gcctttcttt
      241 ttccggccgg ttcgccgatg gcgagcctgg gaacctaccg cttcccgccc ccgccggact
      301 ccatccactc gtccaggaag ctcctcaggt gctccgagcg gctcgggtgg cgcagctttc
      361 taagagcctt ggcctcgatc tggcgaatgc gctcgcgggt gacgtcgaac tgcttgccca
      421 cttcctcgag ggtgtggtcg gtgttcatgt cgatgccgaa gcgcatgcgc agcaccttgg
      481 cctcgcgcgc ggtgaggccg ccgagaactt cccgggtggc ttccttgagc ccctcgccgg
      541 tggcgaggtc cacgggcgaa ccgatggtgc tgtcctcaat gaagtcgccc agatgcgaat
      601 cctcgtcgtc gccgatgggc gtttccatgg agatcggctc cttggcgatc ttcagcaccc
      661 gccgcacctt gtcctcgggc atgtccatgc ggtcaccgag ctcttccggc gtcggctccc
      721 ggcccatctc ctggagcatc tgccgcgaga cgcggttcag cttgttgatg gtctcgatca
      781 tgtgcaccgg gatacggatg gtccgggcct gatccgcaat ggagcgggtg atggcctggc
      841 ggatccacca ggtggcgtaa gtagagaact tgtagccgcg gcgatactcg aacttgtcca
      901 cggccttcat caggccgatg ttgccttcct gaatcaggtc caggaactgc aggccgcggt
      961 tggtgtactt cttggcgatg gagatcacca gacgcaggtt cgcctccacc atgtccttct
     1021 tggcgcggcg ggctttcgcc tcacccagcg acacccgccg gttgatgtcc ttgatctcgc
     1081 caacggtgag gcccgtttcc ttcaccagcg ctttcagctt cttctgagcc cgggcgatct
     1141 cgtccttctg cgcgtccaga cccttcgagt aatccttgcc ggccttgatg tgcttgttca
     1201 cccaggcaac gctggtctcc gagcccacgt actccttccg gaagacggcg cgaggcatcc
     1261 gcgcctgacg cacacaggcg ttcatgatga tgcgctcatg acgccggacg atggcgagcg
     1321 ccatccgcgg catgtccacc agctcatcga agtggcgcgg aaccagcttg aagggcgaaa
     1381 acgccacgcc cagcttctcc agctgagcct gggcttcctt gctgttgcgg cctttctccg
     1441 caatgacctt gtacgtcttg ttgtactggc gcttcaacgc ggtaaagcgg cgctttgcct
     1501 ccaccggatc cggacccttg tcctcttcct cgtcgtcatc gtccttctgg ttctgagcgc
     1561 ccggctgcac ctgagccgcc tgaggaacgt tgtcggtggg gtcgagatag ccaacgagca
     1621 tgtcgctcaa gcgcccttcc gcattggcct cggagtaaac ctccaggagg tactccacgg
     1681 tgccggggaa ataggccatg gcggagagca tgtcgcggat gccctcctcg atgcgcttgg
     1741 caatggcgat ctcgccttcg cgggtaagaa gctccaccgt gcccatctcg cgcatgtaca
     1801 tgcgcaccgg gtcggtggta cggcccgctt ccgtctccac cgcggcgagc gcggcggccg
     1861 cttcctcggc agcgagctcg tctgccgtat tctcctccgt ggagagaagc tcgtcggcat
     1921 cgggcgcggt ctcgtacacc gtgatgccca tgtcgttgat catgcggatg atgtcttcga
     1981 tctgatcagg atcggagata tcgtcaggaa gatggtcgtt cacctctgaa taggtgatat
     2041 agccctgctc cttgcccttg gcaatgagct ccttcagccg ggattgctgc tgttcaatct
     2101 gcgtgctcat gacacctcaa atagtgaaat gcgttggggc agagaggttc cccgcattac
     2161 agcatccctg acttaatgct gtggtcgtgg gttcccgccc ggattcgcgg actgccgaaa
     2221 atgagttaac agccgaatat accatatata ggggcgctta ctgaaagtta aacaattgtc
     2281 cgacaaaaaa gtcgcttgcg caccgcctgg ctaatcggtg ccgctgacct tgtctcgagg
     2341 gccctgcttt aaagaccaat agtccttcag gcgctccgcc gacggatcct ggtgcatctc
     2401 ggagatcagc tgccgcctgg cagacgcctg ctgcatctcc agatatcggg ccacgccctc
     2461 ggtgaaatcg ccacgcaccg ccgcgatgtc gatggccgac ggctgccggg caagacccgt
     2521 cagctcgtcg tgaagatcgt gccccgtcca gcgcccgagc agctccggcg tctcggcatc
     2581 cgggtgctcg tccagatagg tcacgagttc ggccagtaga tcgttccccg ccagcggccg
     2641 cagggcccgg cgggcctctt ccgaaagggc tgagaccacg gacggttcgc gcagcagata
     2701 cccgaccagg cgtctcgcca ggggcgtcag gctgacatcg gcagccgtcc ggcgcgccgg
     2761 ccccctcgat ggtcgcgcct gggcgctcgg ccggaagccc gtcagcccct ccagccggga
     2821 ggccatgagc tcctttagga tgcccgcggg caaccggtcg atgtgcggca tggccaggct
     2881 cgcgaacctg gccttgtcat ccacgctgcc gagatcgagg ccttcctcga gccggccgaa
     2941 aagatactcc actgccggca ccgcccgggc ggccagctcg agatatccgg cctttccgcg
     3001 ggcgcgaacg agagagtccg gatcctcgcc gtccggcaga aacatgaact tcagcccacg
     3061 gccgtcgctc aaggccggca gggcgctctc cagcgcccgc cacgccgcct ggcgcccggc
     3121 cgcatcgccg tcgaagcagc agatcacctc ccccgtgtag cggtaaaggc tctgaaaatg
     3181 ctcctcggta gccgccgtgc cgaggctggc caccgcgttg gatatgcccg cctgggccag
     3241 cgccaccacg tccatgtagc cttccaccac gatcaggcgg tcgatccggc gcagggcctg
     3301 gcgcgcctcg tagagaccgt agagctcccg gcccttgtgg aagacggggg tctcggggct
     3361 gttgagatac ttcggccctc cgccatcggc catcacccgc ccgccgaagc cgatgatgcg
     3421 gccccgcgtg tcgcggatgg gaaacatgat gcggccacga aaccggtcat aggcccgacc
     3481 cttctcgccc cgggtaagga gcccggcttc caggagcttg gcttccccca tgccggcaag
     3541 cgcgctcgcg gccccctgcc actcgtccgg cgcgtacccg atcccgaagt cccgcgcgat
     3601 ggtgccggtg accccccggg ccttgaggta ctcgatggcc tcaggcgact ggcgcagcgc
     3661 cttgcggaag taacgatccg cggcggcgag cacgtcgtag agatcggtgt tatcccgcgc
     3721 cggccgcccc ccttgctccc gcggaacctc gacgccaacc agctgggcca gcgcttccac
     3781 cgcttccacg aactccatgc ggtcgtgctc catgaggaac gtcagcgccg tgccgctctc
     3841 gccgcagccg aagcagtgat agaactgctt ctccgggctc acggtgaagg agggcgtttt
     3901 ctcgttgtgg aacgggcaga gcgcctgata gtcgcgcccg gcctttctca gctgcaggcg
     3961 cgcgtcgatc acatccacga tgtccacccg gttcaggagg tcattgatga aggattgagg
     4021 tatgcgaccg gccatcgcag tcagattagc aacgaattct tcgggagtct gcaatcggct
     4081 gcctgacgac gcgctttaaa tgtgatatgt ctagacatac gaatcgcagg ggaccagtca
     4141 tgagaaccac cgtgagactg ccgggagagc tgctgaccaa agccaaagag ttcgcacatg
     4201 aacgcgggac gacgctcacg gcgctgatcg aggagggcct cataatggtt cttgaacgcg
     4261 cgccgaaggc gggaaagatg aaacctgttc gagtacccgt ctccaagtcg actggcggcc
     4321 tgcagccagg agtggatctg agcgattcgg ccgctctgct ggatgccatg gagcaaggtt
     4381 gaagctcgcc gacgtcaacg tcctggtggg cgcgttccgg accgactcat cgcatcacga
     4441 gagatgccga agctggctga agagcacggt caacgcgccc gccagcttcg ggctcgccgc
     4501 gcaggtgctg tccggcgtca tccgcgttac gacgcacccg aaagtcttcg taaacccgag
     4561 cccgatcgac gaatcaattc gtttcgcgaa cttcttactc gggcgaccga acgccatcgt
     4621 cgtcagccca ggcgagcgtc actggacgat cttcacggac ctgtgccgat ctggcggtat
     4681 tcaaggaaat ctggtgcccg atgcctggtt cgcggctctt gccatagagt cggggtgcga
     4741 gtgggtgacc ctgaacggga acttcgcccg tttcgagggc ctcagctgga gcgcgccctg
     4801 agcgccctca gccctcccgg ccgtagatgt cgtcgaaacg gacgatgtcg tcctcgccga
     4861 ggtaggagcc tacctggacc tcgatgagct ctaaggtgag cttgcccgga ttggccagcc
     4921 ggtgggtcac cccgagcggg atgtaagtgc tttggttttc cgccagggtg atcgtctcgt
     4981 cgccgcgggt gacctcggcg gtcccccgca ccacgatcca gtgctcggcg cgatgatggt
     5041 gcatctgcag cgaaaggctc gcgcccggct tcaccttgat ccgcttgacc tggtaacgct
     5101 cgccggcacc cacggactcg tagctgcccc agggccggaa cacctcgcgg tgatggacgt
     5161 actcgtcgcg gccggccgcc ttgagctgct cgaccaccgc gcgcacgtcc tggacccggt
     5221 ccttgtccgc cactaaaacg gcgtctgagg tttccaccac caccaggtcc gagacgccaa
     5281 tggtgccaac cagccgatcg cgggccgaga cgaaagagtt ctgcgtatcg acgctgatga
     5341 cgtcgccctc caggcggttg ccgtttccgt cccgatccga cgcctcccag atggcgctcc
     5401 aggaacccac gtcgctccag tcgaagccgg ccgggaccac catggctcgg tcggtcttct
     5461 ccatcaccgc atagtcgatg gagtccgcgg ggcaatcgag gaactcgggc cccggtcgga
     5521 aaaagtcgag atcttcggcc ccgcgctcga aggccagcga cgccttctcc agcatcaaag
     5581 ggctgtgccg gccgagctca tccagatacg cccgggcatt gagcaggaac atgccgctgt
     5641 tccagaaata gtcgcccgcg gcgaggtact cgttcgccgt ggcgagatcg ggcttctcca
     5701 cgaaggatcg gacccgcacg acgccggaac ccaactcggc gccgggtgcc tcgatgtagc
     5761 cgtagccggt ctccgggctg gtcggcacca cgccgaacgt gaccagtgcg ccttcacccg
     5821 cggcccgggc ggcgagctcg gcagcagacg cgaacccgtc caggtcggtg atcagatgat
     5881 ccgacggcag cacgagcagc aacggtgccg aatcgggccc cgcctcctcg caggcggcaa
     5941 gggcagccag cgcgatggcc ggcgccgtgt tgcgcccctc gggctccaag atgatgcgcc
     6001 gccaggactg gccgatggcc cggcactgct cggcgaccat gaagcggtgt tcttcgttgc
     6061 acacgatgat gggcgcttcg cccgtggtac ggtctgcccg cttgagcgtc gactgcagaa
     6121 gggagtcgtc gccgagaagg gcgtggaact gcttggggaa aagctctctg gagagcggcc
     6181 agagccggct gccggttcct ccggccagaa gtacgggaat cactttttcc atcgctcgct
     6241 cgtcactccg cacgacggca ttgccgcgcc aagataccag atcctcggat tatcaccgtg
     6301 ggcggatttg taagtgaggc cccggcgctg ccaactaacc tgctgactaa cccgctaaaa
     6361 gcgctttgac gcgaccgctg accgcgccca tgtctgcgcg gccctgaagc cggtctttca
     6421 gcaggcccat gaccttgccc atgtcgcgca tggattccgc attggcctcg gcgatggcct
     6481 ggtcgatggc gctcgcgaga tccgcctcgg agagctgctc cggcatgaac tcgccgatca
     6541 ggtcgatctc gaagctctcc tggtcggcca gatccttgcg cccggcctgg gtgtactgct
     6601 ccagggcatc ctgacgctgt ttgagcatgc ggttgagcac ggccagcact tcggcgtcgg
     6661 tggaggccgc acggcgctcg tcgatctccg ctcgcttgat gtccgcattg accaggcgca
     6721 gcactgccag gcgctgcttg tcgcgcgcct tcatggcggc cgtggtggcc gcggtgatgg
     6781 cttgccttaa ctcggagatg gggagatcgg acacggcgtt cccctgtcgt gcggtgtcag
     6841 aaggactttt cgaagcgccg ggcttcccgc tgcagcttct tcagatggcg cttgactgcc
     6901 gccgccgcct tgcgctttcg aaccgacgtc ggcttctcgt aatactctcg tctccgcacc
     6961 tcggaaagca cgccggcctt ctcgcaggaa cgcttgaatc gcctgagagc gacatcgaac
     7021 ggctcattct ctttgacttt tacgctgggc atgttgtgcg cgaaatcccc tcgaacctga
     7081 cggttgttgc tgaaaagggc gcggatttta ctggcgccgg acggtttttg caaaacgccg
     7141 gaatcgttac catccgccca tgctggtcct cggaatcgaa acttcctgcg atgagacggg
     7201 cgtcgcgctc tacgacggtg tcgaggggct gctggcggac gctctgcaca gccaggtgga
     7261 agtgcacgcc gcttacggcg gcgtggtgcc cgagctggcc tccagagacc acatccgcaa
     7321 gctggttcct cttgtccgca agacgctgga cgattccgga cgctccctcg cggacgttga
     7381 cggcgtcgcc tacacgtccg gccccgggct catgggcgcg ttgctggtgg gcgcgggttt
     7441 tgcgaggagc ctggcctggt cgctgggcaa accggccgtc gcggtgcatc acatggaggc
     7501 gcacctgctg gccccgctga tgaatccgga cacggccccg cctatgccgt tcatcgccct
     7561 gctcgtttcc ggaggacaca cccagctggt gctggtcaac gcgatcggcg attacgagct
     7621 cctcggtgag tccgtggacg acgccgccgg ggaggccttc gacaaggcgg cgaagatgct
     7681 ggatctcggc tatcccggag gtcccaacct ggcgcgcgcc gcggaagggg gcgatccgca
     7741 ccgattcacg ttcccgagac ccatgaccga ccgccccggg ctcgacttca gcttcagcgg
     7801 attgaagacc tacaccctga acacgacccg cgacctggcc cccatgagcg acgcagaccg
     7861 cgccgacgtc gcgcgcgcgt tcgaggaggc cgtggtggat accctctaca tcaagtgccg
     7921 ccgggcggtg gagcagacgg gcatcaccca tctggtaatg gccggcggcg tggcggccaa
     7981 cgcccggctg cgcagaaagc tcagcgaaga cttgcgggcc gccgtctact accccccggc
     8041 gcggctgtgc accgacaacg gagcgatgat cgcctacgcc ggctgcctgc gccttcaggc
     8101 gggcgagcga gcgccccttg ccatcgagac ccgtgcgcgc tggcccatcg tcgagctcgg
     8161 ccgccccggc ccgccggcct gacccatgga cagcgtcttc gtcaggaacc tgaccgtctc
     8221 caccatcgtg ggcgtctacc ccgaagaacg cacccggcct caaagggtga tcgtctcctt
     8281 cgagctgttt gccgataccc gggcagccgc ccaaagcgat gacctggaag acgccctcga
     8341 ctacggcgaa gcggcgagcc gggtcgcgga gttcgtccgc ggcagccgct tcgagctcat
     8401 cgaagcgctc gccgacggca tcgcggcgct gctgctggaa ggctatcccg ccgcgcgcgt
     8461 ggtggtggaa gtgctcaagc ccggggcggt cacagacgcg gagacggtgg gcgtgaaaat
     8521 cgagcgttcg cgggcctgac ctgtcgccca acgaatcaac acatcggcca gaggcacgcc
     8581 tcgacccggc ctacctgccg acgttcgaac gctgggcctg gcgaatggcc tgcgtgcgcg
     8641 cctggtcgat ggcctcgccc agagcggctc cggtcaggga gcctccaacg ctggccgtcg
     8701 tcacctcctg acgaatcgag gccgccagct cgagcagcgg ctcgggattg cgccccgcgc
     8761 aggcggccga aacgcgcagc gccatctctg gatcgtcacc cgccttgaat gcccccacgg
     8821 acttcagcgc cgcgcacagg cttcccgcct cggcggtctc ccaggcagca agtaccaccc
     8881 cgtgagaggc gatgtggccc atggcccggc ggtgtcggcg cggcgcccga agccgttcac
     8941 atagcgtatc taccgcctcc ggggcgagat gccagccaag ggcggcgaac cgaccgagcg
     9001 cgtcttcgat tgcttccggg aaggtgatct cgacttcggc gagctcggcg aaccacgtcg
     9061 acaagccgcc cacggatgcc agaacggtga aaaagcgcag gggcgccttc gatcccagtg
     9121 ccttgaccag ctcctgccag acccgctccg cggagagctc cgccagcgcg ccgctgccgg
     9181 cgagctcggc catgagagcc cgggtctctt cggcgacggt gaaatcatcg agctgggcgg
     9241 cgaaccgggc cacccgaaat acccggagcg gatcctcgga gaaagcgacc gagacgtgcc
     9301 tgagcacttt cgcttccagg tccgatcgac cgccgaaggg atcgatcagg gagccgtccg
     9361 acgcccgcgc gatggcgttc acggtgaggt cgcggcgctc caggtcctgc tccagagtga
     9421 cgtcgggccc ggcgtgacac tcgaaacctt tgtgccccgg ccccgtcttg cgctcggtgc
     9481 gggccagcgc gtactcctcg ctggtgtccg ggtgcagaaa gaccgggaaa tcacggccca
     9541 cctgccgaaa gccgcgtttc agcatcgcct cgggggaggc gcccaccacc acccagtcgc
     9601 gctcgcgcac cgggcggccg agcagctcat cgcggaccgc gccgccgacc agatatacgt
     9661 ccatggctca ttctgagaaa gtcagcccac gaaggggccg gtgacctcgt aagtgatgcc
     9721 gcccgtcacc cgaccggaaa agccgcgctg ggagctgaag taaaggcgtg acatgtcggg
     9781 cgtgaacgcc aggccggtga tttccgatcc gccctggcga gtgacctgca gcagggcaac
     9841 cggctcgccc tcccggggga gaacgacgat ctgcatgtca tcgccgtctt cggccaccag
     9901 cacgtcgccg gatgcattgg tttccacgtt gtcgacgccg ccaagtatgg gcgttgcggc
     9961 cgtggcctga tcgtagatca cctccaggcg atcctcgccg agatcgagag cccagacccg
    10021 gttgtcgccc ttggtcgcga gatagaccac accgtcgcag tagctcatcc cctcgccgcc
    10081 gtcgaagccg gtgctttcag gcacctgcca gcgggtgggc ttgcggcccg cttcggggtc
    10141 cggaacgcgc agccacacca ccgagccgga gccgtccgga gcgagtgaag cgacctcgag
    10201 gacgccagcg ctcagatcgg gccgctcgag atgcctgggc aacgtgggcc ggaagcggta
    10261 gaggcgcccg tcgggctcat cctcgctcat gtagacggta ccggtgagcg gatcgaccac
    10321 ggcagcctcg tgctggaatc gtcccagcgc cggcagcgcc accggttgcc gcaatcccaa
    10381 cgggtcgcat tcatataccc ggccgcggcg aacttcctcg cagctcaacc aggttcccca
    10441 cggcgtcgcc ccgccagcgc agttgccgct cgttcggccg aggatcgggt aggcgtctac
    10501 cagggttccc gactcatcga accgcagggc gctggcaccg ccgaacccca gcggcatctc
    10561 gcagttgctg acgtagaccc aacctccttc gggatgcgca aacgtagcac ctccatccgg
    10621 ggccgcgtgc caggcgtagg acgaggacga cacggggcga ttgccggact cggcaacgat
    10681 cctcgagctg aacccggcag gcagccggat accgttgtga tccggggcaa gcagctcgcc
    10741 caggcggccc atcgcggacc cagggcccag tctgtccgcc caattggcag acgacgaccc
    10801 accttgggta gggcaggcgc gagcgagcga gggcctcagc agatacgcgg cgccgatgcc
    10861 ggcaatggtc aggaaagatc gtctgtccat ctcggcattg taacaggggc tacgccgccg
    10921 actgagcctc ctccgcgggg caggcggtgg tggcaaccac ctcgtcgtgc cagacggact
    10981 ccaggcgaac gggaaatccc cagagccgat gcaggtgccg catgacctcg tcggtgcttt
    11041 gacccatggg ctggcgcgca tgctgcgcgt gccgcagcgt gaggctccgg tcacccctga
    11101 cgtcgacccg caccacctga atgttcggct cccggtcgcc aatgttgtac tgcccggcca
    11161 gaaggctgcg cacctcgcgg tagccgcccc gatcgtgaat ggccgagatg agcacctcgt
    11221 cctcggcgtc gtcgtcgacg acgccgacca gacgcatctc gcgcatgacc ctggggctta
    11281 agaactgcag cacgaagctc tcgtccttga agtcccgcat ggcggccggc agcacctcga
    11341 gccagttgcc cccggcgatg tcgggaaacc actcgcgatc ctcgtccgtc ggatgctcgc
    11401 acacgcgccg cagatccgag tagatggcaa agcccagcgc gtagggattg atgccccggt
    11461 acgcggggtg atcaaaactc ggctggaaca gaaccgctgt gtgcgaggcg agaaactcca
    11521 tcatgaagcc gtcggtgacc aggccgcgat cgtagagctc gttcaaaagg gtgtaatgcc
    11581 agaacgacgc ccacccctcg ttcatcacct gggtctgccg ctgagggtag aagtactgcg
    11641 ccaccttccg gacgatgcgc acgatctcgc gctgccaggg ctccaagagc ggcgcgtgct
    11701 tctcgatgaa gtagaggaga ttttcctgag gctcccgcgg gaagacttcc tcgtcgctcg
    11761 agtcgagctg ccgggcctgg ggaagcgtcc gccacagatc gttgagcgtg cgctggacgt
    11821 actcggcgcg ttcggcctgg cgccgcttct cttcctctgc cgagatcggg tacggccgct
    11881 tgtagcggtc gaccccgtag ttcatcaggg cgtgacagga gtcgaggatc atctccactt
    11941 cctgcacgcc gtagcgctcc tcgcagctgg caatgtagtt acgggcgaag agcaggtagt
    12001 cgacgatggc gctggcgtcc gtccaggtct ggaacaggta gttccccttg aagaaagagt
    12061 tgtggccata gcaggcatgg gcgatgacca gggcctgcat ggtcatgctg ttctcttcca
    12121 tgaggtagga aatgcaggga ttcgagttga tcacgatctc gtaggccagg cccatgaatc
    12181 cgcgctggta gctgcgctcg acgtcgacga agtgcttgcc gaaggaccag tggtgatagc
    12241 cgatgggcat gccgatggag gcataggcat ccatcatctg gtccgaacgg atgatctcga
    12301 tctgattggg gtacgtgtcg agaccgaact gctcggcaat cgcgccgatc tcgcggtcgt
    12361 accgaccgat gagctcgaac gtccagtccc cgccgtcgga gatgggctcg cgcggccccc
    12421 tgcccggcaa gaggatttcc atcacgcggc gtccttcttc tggaagaggt cgtgaaacac
    12481 gggaaagatg tccgacacgt cggtgatctg gcgctgggca aacgattccg gaaaccgctc
    12541 ggccaggtgc tcgtactccc gccacagcgc ctgatggctg cgcttggtga tctccacgta
    12601 ggagaagtac tgcaccagag gcaggatctt cttctccaga agctgccggc agaggggcga
    12661 gtcgtcgttc cagttgtccc cgtccgaggc ctgggccccg taaatgttcc actcgttggg
    12721 cgagtaccga tcttccagca cctcgagcat cagcttgagc gcgctggaga ccacggtccc
    12781 gcccgtttcc
//
LOCUS       DBNHLM010000079        57790 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_788935, whole genome shotgun sequence.
ACCESSION   DBNHLM010000079 DBNHLM010000000
VERSION     DBNHLM010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 57790)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 57790)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57790
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_788935"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="AC16QF_03615"
     CDS             <1..752
                     /locus_tag="AC16QF_03615"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6447668.1"
                     /translation="GARRKIPAFVYMPDGPGPHPVVIHLHGGPHSQYRPKFSASAQYY
                     VKELGLAVVAPNFRGSSGYGKNYLKLDDGYLREDAVRDVGALLDWIAAQPRLDADRVA
                     VSGGSYGGYMALASLIRYGDRLVAGVSRVGISNFVTFLENTRAYRRDLRRVEYGDERD
                     PQMRAFLEGISPLGQADRIDSPLLVIHGFNDPRVPASESEQIADAVSAAGTPVWYVLA
                     MDEGHGFRKKANSDYAAAVTALFLQRYLLDD"
     gene            775..1785
                     /locus_tag="AC16QF_03620"
     CDS             775..1785
                     /locus_tag="AC16QF_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013663.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR6447669.1"
                     /translation="MAICVNPMSGRDVRRLAARASNMTHEAKRDMVARVAAGAQAMGA
                     SDIYVTREPFRIASLALEHMGLAAAVHVLQTPLTNGPADTEAAVAAFLAAGCRVIVSL
                     GGDGTNRAIARALGDAGAAGAGVDLIPLSTGTNNVFPVLAEPTAAGMVAGLIASGRLP
                     DDDLRQTAKVIHLSGTDARGRAVRDLALVDAALLRDDHVGNLLPFAAQRLARIVLTRA
                     EPDAVGMSPIGGMLDVVEAGDDEGLLVEMGPGREFSAPLSPGAFQAVSVRSASRLPLD
                     VTVPLAGPGVIALDGDRDYKIAPREALTARIRRDGPRVVDIAAAMRRAVARGIMAPAR
                     NG"
     gene            1790..2983
                     /gene="fabV"
                     /locus_tag="AC16QF_03625"
     CDS             1790..2983
                     /gene="fabV"
                     /locus_tag="AC16QF_03625"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019820659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabV"
                     /protein_id="HIR6447670.1"
                     /translation="MIITPRIRGFICTTAHPAGCAAHVKEQIDYVSGQAALVGGPVVR
                     NALVIGASGGYGLASRIVAAFGCGAATLGVSLEKMPSETRTASAGWYNNVAFEEAAAA
                     AGLYAKTLDGDAFSDEMKGRVIDTLKADMGPIDLLIYSLASPVRQHPRTGELHRSVIK
                     PLGQALHMKSVHVDRGEVVEVDLEPADPEETAATVAVMGGEDWEFWIDALKDAGLLAP
                     GFRTLAFTYIGTELTWPIYWEGTLGKAKEDLDRAAGAISERLEDLEGNARVAVLKAVV
                     TQAMSAIPVVPLYASLLFKVMKERGVHEDPIQHIHRMFATQLCTGGALRLDDAGRIRM
                     DDLEMTDAVQEDVRDRWERVTTENLSELGDLDGFREEFLKIFGFGIEGVDYDADLDPT
                     LGRAP"
     gene            3080..4327
                     /locus_tag="AC16QF_03630"
     CDS             3080..4327
                     /locus_tag="AC16QF_03630"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR6447671.1"
                     /translation="MALRNLHLRAIPFRYAGYSGLALTILALQGTPRPAWALGVGAAG
                     CLIWTIAAHGAGRLAQTRGTSTVPFALGLHLAEALLAGALIGWLSLPWLPLLVAITVL
                     LASNAAQAGWWLAWRAGIAVAVGIGAGRLLAVAPVMSSTPAADLTASVLLLGYTVGLG
                     LTSFAKAQRLHAAQQDLARRALVTERLNSRLGRYLPPPVRARVRRRPDERCGLERRWL
                     TIAYVDVVGFTELAGRLAPEELAVVVNDYFAASGRLFASSGGTLAGLQGDGLLAYFGN
                     ATAATRGRAAVDCVESCLRVSRLLRELSECWQRRGYVTTLTTRCGVASGYCTVGDWGE
                     ERLDFTVIGAPVNLASRLQAHAGHDGILICEPTAALVGQVFPLGAERELVLRGIGKVK
                     ARRLESALVDPPPASAKVPRPEV"
     gene            4333..5373
                     /gene="hemE"
                     /locus_tag="AC16QF_03635"
     CDS             4333..5373
                     /gene="hemE"
                     /locus_tag="AC16QF_03635"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HIR6447672.1"
                     /translation="MTKTFLQACRGEATPHTPIWLNRQAGRYMPEYHQVKGDMPSLDF
                     FKNPERAARATLDAQRILGVDAAILFADLLPILEPMGLRLDYQPGVGPVFDNPIRSTA
                     DVDGLVSLPAEEATPYIAETVTNILEELPGEVALIGFAGAPFTLASYAIEGRGSRNYV
                     FVKKLMYEHEAVWHRLLDKLVEQVASYLHLQIRAGVDAVQLFDTWVGCLSVADFRRYV
                     LPHTRRLIQAIKEGSGGRVPVIYFGTGNSHLLADVVGAGPDVLALDWRVPLGETWKAL
                     GCTSIQGNLDPIVLCADRETVAEQAQAVLDQAAGRNGHIFNLGHGIIPETPVDNVKFL
                     VDFVHERSARSA"
     gene            5348..5776
                     /locus_tag="AC16QF_03640"
     CDS             5348..5776
                     /locus_tag="AC16QF_03640"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR6447673.1"
                     /translation="MSDRRVAPERIAAGLLLAAFWLPLAVCTYLALHPSPPDAVFRLS
                     DVLLHGFAFTYLTLALALAHGHRHRLLPGLWMLGYGVLIELLQSFETSRSAEVGDVLV
                     DVAGIGLGLLAYGWAGAFLRALAVRIASLVVGAGASNADS"
     gene            complement(5822..6271)
                     /locus_tag="AC16QF_03645"
     CDS             complement(5822..6271)
                     /locus_tag="AC16QF_03645"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR6447674.1"
                     /translation="MLDKGDTAPDFELPDQAGEPIKLATLLDSGPVVLYFYPADFTAV
                     CTREACAFRDRHEDLSNLDVRIVGISPQNQASHRRFAETYGLPFPLLCDTSKKVIRAY
                     GVDGPLGLGVRRVTYLIGRDRIIANRAVSDIFLGSHLALIEEVLGQE"
     gene            complement(6284..6862)
                     /locus_tag="AC16QF_03650"
     CDS             complement(6284..6862)
                     /locus_tag="AC16QF_03650"
                     /inference="COORDINATES: protein motif:HMM:NF047247.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HIR6447675.1"
                     /translation="MRLPYLLLLALITAMSFPGVGAEPPPAATEFPVPVVEAAGDFNG
                     VLARTAQVYIGGQPDASALTRMRALGVDTIVNLRTAAEMTNRQQVPFDEKAKAESLGM
                     RYVHIPAGGPKSPYSPSAVDQLAAAIAGTEGKVLLHCASGRRASHLWVAYLVRHEGLT
                     LEEAMSHGRAVNFGELPLEGFLGEPISFELAR"
     gene            complement(6862..7164)
                     /locus_tag="AC16QF_03655"
     CDS             complement(6862..7164)
                     /locus_tag="AC16QF_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447676.1"
                     /translation="MEIELKNGRVIHARPDATGSCAILTGPAPSEPRRWRDLTTDLPF
                     AFGRDAVISLAEPIEGEQTIVGLRVVFADGAELVYDVSGGRPRLTTSTDEISGARP"
     gene            complement(7263..7574)
                     /locus_tag="AC16QF_03660"
     CDS             complement(7263..7574)
                     /locus_tag="AC16QF_03660"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6447677.1"
                     /translation="MMDLQSWREDIDGELNPDNMRAKLAVGGYAVAMYTYPPGTRFGN
                     HTHAGDKKDGVLSGQFLMEMLGERVTLKAGDILHVPAGVVHCASVLGEEPVVSLDATK
                     L"
     gene            complement(7601..7972)
                     /locus_tag="AC16QF_03665"
     CDS             complement(7601..7972)
                     /locus_tag="AC16QF_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HIR6447678.1"
                     /translation="MSALYALLADLLVVLHLLFILFVVLGGFLTIRWPRLAWAHLPAV
                     AWAVVVEWAGWLCPLTPWEARLRSLAGEAGYQGDFVAHYLLPLLYPQAMTRTTQVLLG
                     ALALAVNAAIYGGWWYLRRRR"
     gene            complement(7981..8352)
                     /locus_tag="AC16QF_03670"
     CDS             complement(7981..8352)
                     /locus_tag="AC16QF_03670"
                     /inference="COORDINATES: protein motif:HMM:NF016701.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6447679.1"
                     /translation="MIDGGCFCGEVRYRIEDGDYPAAHCHCTMCRRTSGAPFVSWLVV
                     PKEKFSFTAGTPKQLQSSDRGRRFFCSSCGTPMTCIVDDHPDNVDVTLGSLDEPERFV
                     PRFEIHTDTRLGWVQTDLKQA"
     gene            complement(8362..8622)
                     /locus_tag="AC16QF_03675"
     CDS             complement(8362..8622)
                     /locus_tag="AC16QF_03675"
                     /inference="COORDINATES: protein motif:HMM:NF025715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HIR6447680.1"
                     /translation="MSDEDIRRALQALHTELENADPIDPELVSLLKEVDSDIHRVLEA
                     STPALEGLGDRLEWLAAKLAVSHPRVEGFLRELVDALGKLGI"
     gene            8860..9321
                     /locus_tag="AC16QF_03680"
     CDS             8860..9321
                     /locus_tag="AC16QF_03680"
                     /inference="COORDINATES: protein motif:HMM:NF021525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HIR6447681.1"
                     /translation="MLRFGGVCWLLAIALLLGACDPQDRRPGLWLTGEVADVAPGDWA
                     FSDEYAEIALEVATPYLIPHSVTIWCAQVDGTLYVAAAAPEEKNWPGWVSDDPDVRLK
                     IGDTVYAATLAELEDEAEIAAVRSAYSGKYEVANLGSGASLRYWRVGPRGA"
     gene            complement(9344..9790)
                     /locus_tag="AC16QF_03685"
     CDS             complement(9344..9790)
                     /locus_tag="AC16QF_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447682.1"
                     /translation="MSFLPAGFHVPELLVTPTFSARPLTVDDVVRDYDAVMSSRERLV
                     GTFGSESSWPAADLSLKQNLIDLGWHQKEFQKRRSFAYTLVDPDDTLTIGCFYVYPEA
                     PDGFDASVYLWVRTSHSDMDEAVFSAVKQWLEEAWPFDRLACPGRN"
     gene            complement(9795..10118)
                     /locus_tag="AC16QF_03690"
     CDS             complement(9795..10118)
                     /locus_tag="AC16QF_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447683.1"
                     /translation="MRAVAIIIAAALLAVSPVVTGDRSAAGIRVSESQRYCQAARQCT
                     KVQTRCGGCDCGTAINESFAAEHRDRLAALCGSHEQVECERSCPETRPICVIGVCALE
                     PLRGS"
     gene            complement(10126..10329)
                     /locus_tag="AC16QF_03695"
     CDS             complement(10126..10329)
                     /locus_tag="AC16QF_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447684.1"
                     /translation="MNEIHLLLGLVLSAIGIGYLVYGRRQQAPVFFIAGLGLVSIPFF
                     VGSTSTLLVAGAVVGGAPFLIRL"
     gene            complement(10412..10813)
                     /locus_tag="AC16QF_03700"
     CDS             complement(10412..10813)
                     /locus_tag="AC16QF_03700"
                     /inference="COORDINATES: protein motif:HMM:NF016152.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HIR6447685.1"
                     /translation="MTAEHPPMDGEAARAHLLARPEAREDFPFGPDVYVYKIGPKMFA
                     TLSYVNGVAQMNLKCEPNEAMALRDIFEAVRPGYHMNKAHWNTVTLDGSIPAGELERM
                     MDRSYALVVRGLRKAERTGLEIRHGKEAVYR"
     gene            complement(10810..10992)
                     /locus_tag="AC16QF_03705"
     CDS             complement(10810..10992)
                     /locus_tag="AC16QF_03705"
                     /inference="COORDINATES: protein motif:HMM:NF022662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3012 domain-containing protein"
                     /protein_id="HIR6447686.1"
                     /translation="MHIQTKLSIIVLAAALAACSPEVGSDAWCEDMAEKDKGDWTANE
                     AAEFARSCLFKSYDDE"
     gene            11324..11578
                     /locus_tag="AC16QF_03710"
     CDS             11324..11578
                     /locus_tag="AC16QF_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR6447687.1"
                     /translation="MKLFVGNLPWSVTDQDLEEMFAPFGAVGSAKVITDRDTGRSRGF
                     GFVEMSDDSARNAIADLNESEISGRKLTVNEARDRDRGRR"
     gene            complement(11706..11996)
                     /locus_tag="AC16QF_03715"
     CDS             complement(11706..11996)
                     /locus_tag="AC16QF_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447688.1"
                     /translation="MRAIRGRALIVSAAIGLSACASDPRVEDLGDGRISVDCSGGTPS
                     WTRCQALATEACAGRGFDVVSQVSNEGSANVGRNDWSTEGSQVTRRMIVACR"
     gene            complement(11993..12295)
                     /locus_tag="AC16QF_03720"
     CDS             complement(11993..12295)
                     /locus_tag="AC16QF_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447689.1"
                     /translation="MIDAFNYFVFVLVPIFLLGFQAVLLAVLFFLKARKWLSVYAFAS
                     GGFVAGLGFGYLFATLSGSTQTLLVVCLSVGAFGILSGIFWWFLLVKKASQAQEQA"
     gene            complement(12344..12688)
                     /locus_tag="AC16QF_03725"
     CDS             complement(12344..12688)
                     /locus_tag="AC16QF_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447690.1"
                     /translation="MNLDLTLKAAAILLLLISGMEYLVSSVIHLGPVLFANVMFVTSI
                     FSRTQPRRIAVIALAFAVVIPIGAFRAYVRDDSALWVALLNLAIFCYVAFVAVQTLTA
                     PSGRNIGNGQGS"
     gene            complement(12804..13343)
                     /locus_tag="AC16QF_03730"
     CDS             complement(12804..13343)
                     /locus_tag="AC16QF_03730"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR6447691.1"
                     /translation="MRQISSIPFDIFSVRFKARLALLALGPLLGSAPAALADLKIDRL
                     VIEKSQRVLILEHDGAPVRSYSIALGGAPRGHKQREGDQRTPEGQYTIDARNEDSAFH
                     RSLRISYPNEADRRRAAMEGVNPGGQIMIHGIGEADPEMRKLHPYIDWTAGCVAVTDS
                     EIEEIWALVEIGTPIVIRP"
     gene            complement(13425..13898)
                     /locus_tag="AC16QF_03735"
     CDS             complement(13425..13898)
                     /locus_tag="AC16QF_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447692.1"
                     /translation="MPQHPREPDIASWHRYFAIECNNAAWDLAGRARTAPEDEQMLQA
                     AHAAAFHWDQVGEELNRMRATMLLAKVHALLGKNPSALRYAEEMQTFFLDRDTPDWEI
                     AFAHAIYAHAAYVAGDYTAHARAYRDALQALEVIADPEDHMLVRITFDQVPPPEP"
     gene            complement(13938..15617)
                     /gene="ilvD"
                     /locus_tag="AC16QF_03740"
     CDS             complement(13938..15617)
                     /gene="ilvD"
                     /locus_tag="AC16QF_03740"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017338119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR6447693.1"
                     /translation="MTDQKRPYSSIVVDGVQQAPSRAMLYPVGFTEADFAKPQIGIAS
                     TWSMVTPCNMHIDALAEAAALGADEAGGKSVVFNTITVSDGISMGTPGMRYSLVSREV
                     IADSIETVVGAQGFDGFVAIGGCDKNMPACAMAMARLNRPSVFVYGGTIQPGAERRDI
                     VSVFEAVGAHAAGRISDIELKEVEATAIPGPGSCGGMYTANTMASAIEAMGLSLANSS
                     AQEAISNAKRQDSYQAGAAVFNLIERGIKPSDILCREAFENAITVAIALEGSTNAVLH
                     LLAIAHAAGVPLELDDFSRVGERVPVLADMRPAGQYAMSELIAIGGIQPLMKTLLEEG
                     LLHGDCLTVTGKTLAENLADVAPYPDGQNVIRPMSDPIKKDSHLVVLYGNLAPTGAVA
                     KITGHEGLSFTGSAKCFHGEEAAMAAIMDGAINKGDVVVVRYEGPKGGPGMREMLSPT
                     SAINGRGLSQDVALITDGRFSGGSHGFVIGHITPEAYEGGPIALIEDGDLITVDAEKR
                     EINVQLSDADLAARRARWQKPAPYATKGILAKFARLVSSASEGAVTDKNLD"
     gene            complement(15650..16981)
                     /gene="argA"
                     /locus_tag="AC16QF_03745"
     CDS             complement(15650..16981)
                     /gene="argA"
                     /locus_tag="AC16QF_03745"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003641.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-CoA as donor [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HIR6447694.1"
                     /translation="MSDRPAKAEAENGYARWFRASTPYISAHRNRTFVILLGGDAVAH
                     GNLPNIVHDLALLHVLGARVVVVHGARPQIDAALGESPFHEDRRITDAAAMETIIGIL
                     GQQRARLEALFSTGLPTSPLHNTNISVVGGNFITARPVGIHDGVDHLFTGRPRRVHGG
                     RIQKALDTGALVLLSPLGYSPSGQAFNLAADELAADVAMALDADKLIAFGSDRYVSNE
                     RGEPQPNLTPAQLDTLIDVPALDDTTTRGHLRALLRACRGGVPRCHLVSYAHDGALLE
                     ELFTAGGGGTQISEDHSNLVRQARLEDVAGIVELIRPMEEAGVLVRRPRDRLEQEIDR
                     FFVAEVDGIVMGCCALYPFEDCGELACVAVHESHRSKPGETRLGVALLRATEAAAGAM
                     GLRKLFVLTTQTRDWFVDNGFEDSTLDALPAPKQAMYNYQRNSKVMIKQLR"
     gene            17300..19069
                     /gene="tfpB"
                     /locus_tag="AC16QF_03750"
     CDS             17300..19069
                     /gene="tfpB"
                     /locus_tag="AC16QF_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly ATPase TfpB"
                     /protein_id="HIR6447695.1"
                     /translation="MPLAQEQLIDLPVLLDGLVKEGYISQRQAEDILITPRTKKELSQ
                     HPMEIVADRDFDNRNKPGHKLDLETMTVWLAHQSGQPYLRIDPLDINVGAVTDVMSYA
                     FAQRHNILAVDVTDDEVVIASAQPYMYQWETMLTQTLRGRRIRRVVTNPADLSRYMLE
                     FYTMARSVAKAQDAGMEMSGLANFEQMLELGQMKSPEANDSHIVNIVDWLLQYAFDQR
                     ASDIHIEPRRDKGLIRFRIDGVLHHVYELPQQVNAAVTSRLKILGRMDVAEKRRPQDG
                     RVKTLSPDGDEIELRLSTLPTAFGEKMVLRIFDPDVLLRSFKELGLSGDDYERWHGMT
                     HSAHGIVLVTGPTGSGKTTTLYSTLKQLATSEVNVCTIEDPIEMVEPAFNQMQVQSNI
                     NVTFAAGVRALLRQDPDIIMIGEIRDLETAEMAIQAALTGHLVISTLHTNDAPTAITR
                     LLDLGVPPYMIKATVLGIMAQRLVRTLCPHCKVEEETDAEAWRLLTKPFRAKQPERVF
                     APKGCLECRDTGYMGRVGIYELLPMSEKVQTLVQADTDMDVLRKQAFKDGMLTLRLSG
                     AQKVGSGLTTISEVLRVAPASQD"
     gene            19074..21818
                     /gene="glnE"
                     /locus_tag="AC16QF_03755"
     CDS             19074..21818
                     /gene="glnE"
                     /locus_tag="AC16QF_03755"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HIR6447696.1"
                     /translation="MSSSSVPPELLSKAERTAARLPYSLPADGLRVLALSDFAANVMA
                     REAQWIQDALEADRFSAPLASGEVRATLAAVSQGAEDLGALQQSLRQWRNRYQLWVVW
                     RHLLRMAPLEETCATLSMLADTMLDLALERLYQWATDRQGVPMGESSRSPQRLVVLAL
                     GKLGARELNLSSDIDLVFAYPEPGATTSGTTNQQFFLRLGQQLIQALDAVTADGFAFR
                     VDMRLRPFGASGPLVMHFEGMEDYFAAHGRDWERYAWIKARACAGDVASGEALLQSLR
                     PFVYRRYLDFGAVDALRDMKRRLYSERNDPNDVKLGPGGIRDVEFVVQLQQMVWGGRQ
                     PELQEANLLRVLPRLASQGHLSDADAAALAEGYRFLRDTEHSIQAEGDLQTQSLPASE
                     DSRQRLASSRGFADYGAFESALNGHRIAVEAVFRNAVGELPEEDTPGQRLWSSPDDAA
                     LLGELGFARAEAAGKLLTSLMEARDRPSQSAESRARLDRLMPGLLDRVSLTEDPDLSL
                     ARVVPVLRSVLRRSAYLSLLRENPHTLAHFVDLTAKSLWLAEQLARFPAFFDALLDER
                     QLAALPDKAALQAELHQQVEAAPADDFERRLDVLREFKEHHVFNVSLAEVRGTLPLMK
                     ASDYLTFLAEAVLGESLDLAWNENADRFPEFAEPRPFIIVGYGKLGGIELGPGSDLDL
                     VFIHDLPVGASQFLHRMVRRLLHILTVPTYSGTLYEIDMRLRPSGSSGTMVSSLEAFA
                     EYQRSQAWMWEHQALVRARAVAGDPALAQRFEAVRRELLCEPRDRGPLREEVLKMRAR
                     MAEHHGDAEDLKRGSGGIVDIEFMVQYLVLAWAHEHPALAEFSDNVRILETAERLDLL
                     PANMARNLREAYVGLRSEWHRSVLDLPDSERAARTLMAHRSDVRDAWREVFGIDPENE
                     "
     gene            21829..22770
                     /locus_tag="AC16QF_03760"
     CDS             21829..22770
                     /locus_tag="AC16QF_03760"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR6447697.1"
                     /translation="MTEPTFSDRDGWIWHNGELVPWRDAKVHVLTHTLHYGVGVFEGV
                     RAYQTPSGPAIFRLHEHTRRLFNSAHILGLKIPFSVDEVNAAQVDVFRANALNEGYLR
                     PMVYLGSEAMGLRAKDLSVNLCVAAWAWPDYMDPDSREKGINVRTSSFTRHHVNITMC
                     KAKANGNYINSILALHEALDSGCDEALLLDNEGYVAEGSGENVFIVKDGEVHTPELTS
                     CLDGITRNTIFHLAAEMGIAIRERRITRDEVYIADEAFFTGTAAEVLPIRELDGRMIG
                     SGKRGPVTERFQSAYFDQVRGKRSAFPEWHTPVSGAQ"
     gene            22760..23764
                     /gene="waaF"
                     /locus_tag="AC16QF_03765"
     CDS             22760..23764
                     /gene="waaF"
                     /locus_tag="AC16QF_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017336199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HIR6447698.1"
                     /translation="MPNSGTRILIVAPAWVGDMVMAHSLVQVLIARHGDPGIDMLAPR
                     ATAGLGARMPGVRNVHVLDVEHGELALGARRSSGLTLKDQYDQAIVLPNSFKSALAPA
                     WARIPVRTGWSGEARVGVLNDRRRLNPERYPRMVDRFAALGLDPDAPLPDDIPGPVLE
                     PDAAAAERLLASRRLSVDGGVTALCPGAEFGPAKRWPSVHFAQVASYAAAQGHQVWLV
                     GSPKDAGVCSEVEAQSPAGVVNLAGRTTLLEAVDLLSRADRVVSNDSGLMHVASALGR
                     PVVGVFGSTSTTFTPPLGERATTVSLGIECSPCFQRECPLGHLRCLNDLPPQRVIELL
                     "
     gene            23764..24819
                     /gene="waaC"
                     /locus_tag="AC16QF_03770"
     CDS             23764..24819
                     /gene="waaC"
                     /locus_tag="AC16QF_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008016956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HIR6447699.1"
                     /translation="MHVLLIKMSSMGDVVHALPAVTDAASHGVTFDWVVEEAFADIPA
                     LHPAVGQVMPIAWRRWRSSLRSSGGELARFWEQLRRRRYDLVLDSQGLVKSGVVAALA
                     RGRTKAGFSHASARERAAALAYRRGVRVARNQHAIDRQRELFAAVFGYCVPAGLDYGI
                     ARPAHQASKTNHCVLLHGTTWESKHYPEHLWREVARRARAAGLDVALPWGSEAERARA
                     EAIATTNADVLPALNLAELTTRIAAARLVVGVDSGLAHLSAALDVPTVVIYGSTDSAL
                     TGCRGRRVSNLQAQFPCAPCLRKACGYHGPEEILHQAPVRPACYSRVTPDAVWDAAAR
                     AMADGGRETAPNPGSDL"
     gene            24816..26231
                     /locus_tag="AC16QF_03775"
     CDS             24816..26231
                     /locus_tag="AC16QF_03775"
                     /inference="COORDINATES: protein motif:HMM:NF018047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HIR6447700.1"
                     /translation="MTATGRPLSLAELQAQGRDLPTPFILEGSLNPAPDDGPIECLEV
                     LRVLPGRRIVMRARLGARQCALKLFVGDRAALSARRERAGLELLAASGVDTPELIADL
                     HLPGGGEGILVDWLAGSRPVDEEDVQGLADVAAVLARLHANGAIQHDAHLNNYLRDAD
                     GMLHAIDGDGVRRPARLRRRQELANLALLLAQLPPPDATRLPVVAEAYWRARWGSDPG
                     PDELAALQRRVHAQRAGRMRRYLRKTLRDCSEFHGERKNGRFVVCARNAWTAQMAEFV
                     DRPEDHLVAGTVLKAGNSATVMRLRIGETAYVVKRYNVKGFFHGLRRALRPTPRYRVA
                     WLSGHRLLRLGIATPRPVALLEARQGLATVAYLVMEDLGAARDLAQAVAEEGYTAGVG
                     RAVAGLFRGLRDAGLVHGDTKASNFLVRDASVYLIDLDAMKPGSQTRSRDVARFLANW
                     DDAPQVCESFRGALQEAGVPL"
     gene            26256..28001
                     /locus_tag="AC16QF_03780"
     CDS             26256..28001
                     /locus_tag="AC16QF_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007939655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase family protein"
                     /protein_id="HIR6447701.1"
                     /translation="MPLIILGLSGALNHDPSAALLVDGEIVAAVEEERFLRDKHAKNR
                     MPLESARYCLRAGGISPRDVDVVAFPYAPIALSSAARWHYARRHWYAPDRALDALFNG
                     NRRYRRNRRRVLELGDQLGIDWSRTRFEPVEHHLAHASSAYHLSGFTEKTAIMGVDGK
                     GEYATTFFGYGENGRIHRIKEFYDPDSLSGVYGAMTEYLGFEMLDGEYKVMGMAPYGD
                     PDLYDLGRLIRRARDGFEVDVNYVNTVGLRRHKESGVGFYFSDKLINWLGPRRQGEVA
                     DEPYIHYAASMQKLFEEWCLELMERYLGDVLRETGRLAFAGGGALNVKLNQRIVALDH
                     VRELFVQPAAGDSGTSLGAATYVAASEGQRLSPMTHVFLGPRYDNDDCLQALRARRES
                     LHWRRLDDVPARAAELLAQGHPLAWFQGRMEFGPRALGARSILGCPSTPGIADRINEQ
                     IKFRERWRPFCPSVLDRVAAEIIQTEHPAPFMTITFDVAQEWKSRIPEVVHEDGTARV
                     QIVDPDSNPRYYALLETLETLTGNAVVLNTSLNRRGEPMVCSPEDALNMFCGSDLQFL
                     VLEDFLVSKTDEEAQ"
     gene            complement(27995..28912)
                     /gene="lpxL"
                     /locus_tag="AC16QF_03785"
     CDS             complement(27995..28912)
                     /gene="lpxL"
                     /locus_tag="AC16QF_03785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HIR6447702.1"
                     /translation="MSRRRKSRPRMYSPRVWGTWLIVAIGWLLARLPISAIGGLGRWL
                     GRIAYRLARSRRHIAEVNLRLCFPDLGEAARREMIRGVFQHVAVGALETMVVWLDPDR
                     DLGDRVTIEGLEHLRAAQAEGKGVLLLGGHFTSLDVMANAVSRAVDLDVIYRANKNPV
                     WEWLQVRGRSRYCDGVIERSDVRQTIARLKAGRTIWYAPDQDYGPKHSVFAPFFGAPA
                     ASITATSRLARISGSPVIFASHFRDWNTMTWHVTFSEPLDGFPTSDEVADAARVNQLI
                     ENAIRAHPDQYLWIHRRFKTRPPGEGKLY"
     gene            28976..30400
                     /gene="hldE"
                     /locus_tag="AC16QF_03790"
     CDS             28976..30400
                     /gene="hldE"
                     /locus_tag="AC16QF_03790"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HIR6447703.1"
                     /translation="MNLPSLQGVHVLVVGDLMLDRYWIGPAERVSQEAPVPVVRVERV
                     EEHPGGAANVALNVASLGARCTLIGYVGKDDAAEALVDRLTAAGVACDLVVVDGWSTV
                     VKLRVVSQNQQLIRTDFESALIPGEDHRALLREKVIARLLDASAMVLQDYDKGVIEDP
                     ETLIGSAIAAGVPVVVDPKRKPFARYAGAAILKPNAKELEAALGSWDDDTDLVSLSAA
                     ACRTHRLGAMVITRGAGGMTVVAADGQHRHIPALPADVYDVTGAGDTAAAALAVTTSL
                     GWDADRGAQVANVASGLVVAKAGTAAITGPELALALAGRHRVDRGALRRAQLEDAVAQ
                     ARAAGERIVFTNGCFDILHAGHVAYLEEARALGDRLVVAVNDDDSVARLKGPGRPVNS
                     LERRLRVLAGLASVDWVVGFSEDTPEALLELLKPDVLVKGGDYGPSEVVGADQVIAQG
                     GEVRVLGLVEDCSTTAIVDRIRGA"
     gene            complement(30437..31195)
                     /locus_tag="AC16QF_03795"
     CDS             complement(30437..31195)
                     /locus_tag="AC16QF_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6447704.1"
                     /translation="MQYRPLGNTGIEVPLIGLGTVKLGRDQGVRYPRPFTIPDARAAA
                     ALLKKARELGVNLLDTAPAYGTSEERLGALIGAERDHWIICTKVGEEFSNGVSRFDFS
                     PEHTRRSVERSLERLRTDRLDIVLIHSSGADVEILENLGTLDALRELQASGRVRAIGI
                     SHKTPDGGRLALAAGCDVIMTVLNPADREQAPIVAEAGRIGCGVLVKKALASGFAGPE
                     SLEWVAAQPGVSCIVTGTIDPEHLAANVAAVCPA"
     gene            complement(31197..32366)
                     /locus_tag="AC16QF_03800"
     CDS             complement(31197..32366)
                     /locus_tag="AC16QF_03800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6447705.1"
                     /translation="MSGRDWQTDAIIVGGGIAGLWLANLLVRQGYQVVVLEKDRLGAG
                     QTLASQGMIHGGLKYALTGRLTRASQAIADMPERWRSCLDGTGDLDLSALAPLAERYY
                     LFAESSSLGRLTGFFASRSLRGRIEKVEPHDYPCVFANPAFHGVVYALRDFVLDTGAL
                     VSQLNDTLRGCVFQHAVAPEDLLVEADRVVLRLPDASLTARHLIICAGAGSQPLLDGL
                     GLRRPRMQLRPLHQVVVRHEHPHPMYAHCLTGIRRAEPRLTITSHPDGDHWLWYLGGQ
                     LATDGVKLDAPALIDRARRELEACVPWLSWDDARFETLRIDRAEPEQTRGGRPDEAFA
                     AAVGSCVVCWPTKLSLVPDLGDKVLGLLDPPSAPHPGSPDLGLPTARVGRSPWET"
     gene            32452..33753
                     /gene="waaA"
                     /locus_tag="AC16QF_03805"
     CDS             32452..33753
                     /gene="waaA"
                     /locus_tag="AC16QF_03805"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HIR6447706.1"
                     /translation="MWRILYQVLLPLAYPLVRLRLLWRARREPEYGRRIPERLGRVPA
                     SVPRGVIWFHTVSAGETIAAAPIIDRLLSEFPESDFLVTTMTPTGSQQVIARLGGRVA
                     HCYAPYDFRGGVERFLDAVAPRLLVIFETELWPNLLEAATRRGIPVDLVNARLSERSA
                     RGYARIGALTRRMLGQLQHIACQYPAHAQRFVALGAQPGRVSVLGSVKFDLRLPDDHR
                     ARCAELARRWRLEGRPVWIAGSTHPGEDAPVLAAHDAVRSACPGACLLLVPRHPSRAA
                     DLEKMIQEAGFSVVRQSVAAGAEAGVTVDVVIGDVMGQLLYLYGLSQAAFIGGSLVPV
                     GGHNPIEPALCGQPLLTGPHTFNFPDVVAAFEDSAALLRVGDAAGLAAEVARCLGDAE
                     LRERMAASARRVVAANTGATERLLALLREHVGRVSGGGPLS"
     gene            complement(33746..35134)
                     /locus_tag="AC16QF_03810"
     CDS             complement(33746..35134)
                     /locus_tag="AC16QF_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HIR6447707.1"
                     /translation="MNKPRLIIALLGLAWASAASATDILDAYRDAQSNDPVLGAARAG
                     LDATQELIPQSRSALLPRVAIGGQTSWNERSFPSPVFIEDIGTRVRIPDQEFNEHGWQ
                     AELRQPLFDLEAWFNFRSSQAGVEGAEWGFVSVEQDLIVRTVVAYLNVLRAQDLLDSA
                     KAAEDAVKRQLEQVQQRFDVGLVAITDVLEAQAVYDSAQVVRIQAAGDHDIFFETLRT
                     LTGQPYDSIARISEALPVVDPTPGNEEEWVNTALTGNPTILAANSQLDAAERTLKARR
                     SGHMPTVDATVSTNHFVTGGASFLGGKIDQDVYALSITLPIYQGGLTSSRAREARALA
                     EQARQELLDRQLTVSRDTRNLFRAVATDVVRVRARKKAIESSKSALEATQTGYEVGTR
                     NIVDVLDAQNRLYASEFDYADSRYNYIIDLMQLRQTAGTLNGEDVEQLNVYADSNDPV
                     ERLNSLKARVIN"
     gene            35416..35820
                     /locus_tag="AC16QF_03815"
     CDS             35416..35820
                     /locus_tag="AC16QF_03815"
                     /inference="COORDINATES: protein motif:HMM:NF018552.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HIR6447708.1"
                     /translation="MAECDANYLRLMKLFPSLREEDVSDFAVCIGDMHYEVCIEVQDR
                     GPYTTLVRLSQLPEAPWSTRPALTVRVYHDARSAEVVEYQRGGHFQAVYDYPNRDMRQ
                     PDEKAQVNRFLGEFLAMCLTHGVSVREPEFAS"
     gene            35897..36676
                     /locus_tag="AC16QF_03820"
     CDS             35897..36676
                     /locus_tag="AC16QF_03820"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HIR6447709.1"
                     /translation="MTDLHLQPRESDRLLGVDTADSLRAVLARALAEGRPDALLVTGD
                     VSHEGDEASYRRFAAIVADHYEGPVLCLPGNHDKSAAMREVLGETWALDLQEWSLIGL
                     DSHVDGQVGAGLSETDMAALEGRLAASRGRHVLVATHHPPVDVGCPWLDKDRIQNGAE
                     LLEWLSEHAGVKGMVFGHAHQVIESAYENLALLGTPATCFQFAPRTERFAVDERKPGY
                     RWLRLDAQGGLQTEVGRVDDYPLTLDLSERSRSAGRAPKPN"
     gene            36694..38586
                     /gene="parE"
                     /locus_tag="AC16QF_03825"
     CDS             36694..38586
                     /gene="parE"
                     /locus_tag="AC16QF_03825"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HIR6447710.1"
                     /translation="MADASKYSAESLQVLSGLEPVRRRPGMYTDTSRPNHLVQEVVDN
                     SVDEALAGYAREIEVTLFKDGSVEVVDDGRGMPVDVHPKHKVSGVELILTRLHAGGKF
                     DNENYSFSGGLHGVGVSVVNALSEWLEVEVKREGKVFRQRYKGGKPTSKLKPEGTVGQ
                     RNTGTRVTFKPDAGYFDSVNVAVGRLKHLLRAKAVLCPGLRVSLNVEGKESESWHYEN
                     GLEGYLVNALAGAEVIPEKPFMHAATGNQEGVDWAVQWVLDGGELITESYVNLIPTAQ
                     GGTHVNGLRSGLIDALKEFCEFRDLLPRGVKLTGDDLWDQCSYVLSAKLKDPQFSGQT
                     KEKLSSRQSAVFIGGVAKDAFSLWLNEHPQDGERLAELAISNAQKRVQAAKKVARKKV
                     TAGPALPGKLADCSGQDAERAELFLVEGDSAGGSAKQARDREFQAVMPLRGKILNTWE
                     VDVSQVLDSQEVHDIALAIGVDPGSPDLSGLRYAKVCILADADSDGLHIATLLCALFL
                     RHFRALVDAGHVFVAMPPLYRIDVGKEVFYALDEAEKEGVLDRIAAEKKRGQIQVQRF
                     KGLGEMNPMQLRETTMNPDTRRLVRLELDSPTKTDEVMDMLLAKKRSPDRRAWLEKKG
                     DQASLA"
     gene            38626..40878
                     /gene="parC"
                     /locus_tag="AC16QF_03830"
     CDS             38626..40878
                     /gene="parC"
                     /locus_tag="AC16QF_03830"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HIR6447711.1"
                     /translation="MESMETENPGIETIPLRAYTERAYLDYAMYVINDRALPHIADGL
                     KPVQRRIIFAMSELGLSNQAKFTKSARTVGDVLGKFHPHGDSACYEAMVLMAQPFSFR
                     YPLVDGQGNWGAPDDPKSFAAMRYTEARLSAYAELMLSESKQGTVDFIANFDGTLTEP
                     RLLPARAPFVLLNGSTGIAVGMATDIPPHNMNEVISACIHLLDSPKATVADLMTHIKA
                     PDFPSEAIIITPPAEIREAYETGRGSVKARAVWKKEAGDIVVTALPHQASPAKVLEQI
                     AQQMNAKKLPMLADLRDESDHENPTRLVLVPRSNRVDAERLMSHLFATTDLEKSFRIN
                     LNIIGLDQRPQVKSLQAILKEWLTYRLETVRRRLTFRLDRINERLHILDGLLKAYLNL
                     DEVIRIIREDDHPKASLMKRFTLSEIQANAILDLRLRQLAKLEEDKLKAEKSELESEK
                     ADIEKTLKSKARMKALLKSELVEIAEGYGDARRSPLGAVEDAAAYSEEDLISNEPITV
                     VLSEKGWVRSAKGHETNPEELSYRSGDGYGHAARGRTNDILVFVDSTGRCYTVAAHSL
                     PSARGQGEPLTGRLKPPAGSRFVGVALGPGESKVLLASDAGYGFVGRLSGLVAKNKSG
                     KACLSVPKGGLALPPLLLADQPDLQVVAVTDQGRMLVFPLTEVPELTRGKGNKLLNIP
                     SAAFKSGEERLVAVTVIRPDDELLVLAGQRHLRMKHRDLENYVGERARRGRKLPRGFQ
                     KVDSLAVAGR"
     gene            complement(40842..41963)
                     /locus_tag="AC16QF_03835"
     CDS             complement(40842..41963)
                     /locus_tag="AC16QF_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447712.1"
                     /translation="MKLTSWLGLVKPPPLSLVGACLGSLVSVAVMLSYWALNDPDQLR
                     HDAYGRSMSTGLAQLAIEPLLTHDRITLGVIANQAADIPGVSGVEIVTVNEQALASSG
                     SLSNARVYQSPVTFNDQIMGAVRVALSEPPSHLGDAVAVAAVTLIAAPFLALALLSIR
                     LQPPLPRQPARIEVELHTPRERHFLVAVNLYDQLGMEPTQRVAELNYARIAAGKVAGL
                     YGGDVENLPGTGLLLDFNDGGDADRPWQVLSAAAVLSELLDEEAGDYRVAIHVGDAPV
                     GHRVLLDEQAVGDAALLSALAKPATLAASSVFSACLDRGGELVERTVIEHPLLEELAT
                     VGPDSVLITALSAPQQDLVQHQAAHLAGHRPATASESTF"
     gene            41989..43227
                     /gene="serB"
                     /locus_tag="AC16QF_03840"
     CDS             41989..43227
                     /gene="serB"
                     /locus_tag="AC16QF_03840"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HIR6447713.1"
                     /translation="MSSEIVLVNVSGEDQPGLMAMLTSTLARYQVRVLDVGQAVIHDE
                     LNLGMLLQIISDGNGVEAAEVLSAIEANVLDQGMTARFTPISAGDYADWVKSQGKPRY
                     IVTLLTAGATGEQLAAVTSIVRHHGLNIDAIRRLSGRLPLTSDAGGQRICIEMSLRGV
                     LADAGALKAGLLEAAERLAFDFSVQQDTVYRRNRRLVAFDMDSTLIDAEVIDELAAAH
                     GVGEQVARITERAMRGELKFADSFRERAMLLKGLSEDALREVADGVALNDGARRLIRT
                     LKHFGYKTAIISGGFQYVGERLRDELGIDYVYANSLEIRDGLMSGEVEGEIIDAGRKA
                     ELLKEIARREGISGQQTIAIGDGANDLPMLNAAGLGVAYHAKPMVKETASHAISTFGL
                     DSVLYLMGFSDRDIDEALTG"
     gene            complement(43262..45364)
                     /locus_tag="AC16QF_03845"
     CDS             complement(43262..45364)
                     /locus_tag="AC16QF_03845"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR6447714.1"
                     /translation="MTAQHNPSAPVRLLIAENSENAAQEFDSLLRDAGIPTRSEIIDL
                     PMAVDAMHEADLLLANSSLPELEQIIPRLSDQAPNVPIVLVNYDDATITTTRGMQLGA
                     ADVVPKSEPEHLVLVVKRELEHVLQYQSLSQTRRALQETEQRCQLLLRSSKAAIAYVH
                     DGMHIYANEGYLKLFGFEDVEDLIGLPHMDLLDADSAVALKVAMKQFQRDGEEKEIEF
                     VGQNTAEETIAGTMTLAAAEYEGEHCMQVTVRTGRATDAPATAQAAADDASTANGEGS
                     APADVRLHAFFDAVAGNPRTRSEDAFSAIFVAEIDDYAEVQRSHGLAGALEVCRHVER
                     CLGELIASDALHRLADHQFAFAVTADNEDEVRAEADQLRGTIEASLLEVREKTVRPTI
                     TLAGMEIATEEDQGVRERLEDCLNEAYDVLRTSMEQNEGNCVELKSQGAGDEPESESA
                     RLLRLINEAIDDQRFVLLFQPMISLHGDSDEHYEVLLRMLDDEGKQMLPGAFLRTAIE
                     NNLAGRIDRWVILQAIKSLSAHRAKGNLTRLTINLTSNSVNDPEFLEWLGVAFKAVRL
                     PTDAVVLQITEQDANTYVRQTREFFEQLKQMHCVASLSRFGRLGNSFELLEQIPAEYV
                     KVDGSLIEKIASSSEAREALTEMIHGLQGAGKLTVVPMVESANILSTLWQAGANYVAG
                     HYLQEPSRSMDYDFSADD"
     gene            45624..46190
                     /gene="efp"
                     /locus_tag="AC16QF_03850"
     CDS             45624..46190
                     /gene="efp"
                     /locus_tag="AC16QF_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR6447715.1"
                     /translation="MATVSTNEFRSGLKVLLEGEPCSILENEFVKPGKGQAFNRVKFR
                     NLITGRVWERTFKSGDSLEAADVMELDMEYLYSDGDFWHFMRTDGSYEQSAAPPSAVG
                     DAKDWIKEQEVCQVTLWNDEPISVTPPNFVELQVTETDPGLKGDTAQGGTKPATLSTG
                     ATIKVPLFINVGDVLRVDTRKGEYVSRA"
     gene            46187..47062
                     /locus_tag="AC16QF_03855"
     CDS             46187..47062
                     /locus_tag="AC16QF_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase GenX"
                     /protein_id="HIR6447716.1"
                     /translation="MNAPPPAWRPACSIEALGARAAMLRTIRDFFFERGVIEVQSACL
                     GAHTVTDPAVDSLAVPGIGYLQTSPEYQLKRLLAAGAPSLYQLGPVFRGGEAGRLHNP
                     EFTMLEWYRLGYTDAELMAEVAELIDSVLGAADYERLSYERVVALGGGAESGAERAEL
                     DLAFADGIAALAGRRVFVVDYPADQAILARLRPGRPNVAARFELCVDGVELANGYWEL
                     GDRAAIERRFAEDLAARRQAGSELPEPDGAFLDAMDAGLPECAGVALGVDRLLMIKLG
                     AGSLAEVMAFPADRA"
     gene            complement(47063..47890)
                     /gene="asd"
                     /locus_tag="AC16QF_03860"
     CDS             complement(47063..47890)
                     /gene="asd"
                     /locus_tag="AC16QF_03860"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937125.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HIR6447717.1"
                     /translation="MSAWFAALQRLLPQHELSRFVGALAKSEAPWIRKPFIHGFARAY
                     GVSLDEAVCQELDDFRCFNDFFTRALRQDARPLARDPAAVLCPADGTVSQTGLIRDGQ
                     LLQAKGHRYSLRSLTSEADRAFEGGTFATVYLAPSDYHRVHLPADGTLLATTAIPGAL
                     FSVNAQTETTVEGLFARNERLVCRFRTGHGQMLVVLVGALIVASIETVWAGPESPYQS
                     VTTTSYDDAFARGAEIGRFVLGSTVIVCFEPGRIELLPHIKAGATVRMGEAIGAFCD"
     gene            complement(47887..48630)
                     /locus_tag="AC16QF_03865"
     CDS             complement(47887..48630)
                     /locus_tag="AC16QF_03865"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR6447718.1"
                     /translation="MVHVGDAAGYLQGHVPGAVLVEPGELIGGAPPATGRLPSRERLE
                     ALFSRIGFDPDQEVIVYDDEGGGWAGRMAWTLDVVGHTRWTYLNGGIHSWLAAGLPVE
                     TGPGLPVPPANVTIDIQGEPIAELEDVMAAIEDDDQVVWDVRSREEYLGLRSGSRRAG
                     HVPGAVNVDWMHLKDPERDLRLVDDVRSLLESRGIVPGKRIITHCQTHHRSGLSYMVA
                     RLLGYPRICAYHGSWAEWGNRDDTPVSRS"
     gene            48816..49361
                     /gene="orn"
                     /locus_tag="AC16QF_03870"
     CDS             48816..49361
                     /gene="orn"
                     /locus_tag="AC16QF_03870"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HIR6447719.1"
                     /translation="MAMPTNLVWMDLEMTGLDPETDVIIEMATVITDSDLNILAEGPV
                     LAVWQPQAKLDAMDEWNQTHHGESGLIDRVRASAVSEREAEARTLAFIEGHVERKQAP
                     LCGNTIWQDRRFLTRYMPTLEGYLHYRNIDVSSLKELVARWRPELLKGINKANRHTAL
                     SDIRESIEELRYYRKHFIALS"
     gene            complement(49366..50454)
                     /gene="queG"
                     /locus_tag="AC16QF_03875"
     CDS             complement(49366..50454)
                     /gene="queG"
                     /locus_tag="AC16QF_03875"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIR6447720.1"
                     /translation="MADLAALRKDIETWAGELGFQQIGVTDVDLSAHEPRVRAWLERG
                     FQGTMSYLERNLDKRFHPEKLETETCRVIVARMDYLTDPTSPRAALENPDRAYVSRYA
                     LGRDYHKLIRGRLATLARRIDSAAAGMQGRYRAFTDSAPVLEKALAEKAGLGWIGKNT
                     LLLNSKGGSWFFLGEIYTNLPLPLDEPGEPGEDGAGCGNCNACMTVCPTDAIVAPGQL
                     DARRCISYLTIELKEAIPEPLRAPIGNRIFGCDDCQIYCPWNRFAGVTNEPDFQPRHG
                     LEAATLLDLFGWSPETFERNTRGSAIRRISWDQWRRNLAVALGNGSPSAAALGALREA
                     RAGASAMVCEHIDWAIKRLTRRSGGSEP"
     gene            50522..52021
                     /locus_tag="AC16QF_03880"
     CDS             50522..52021
                     /locus_tag="AC16QF_03880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR6447721.1"
                     /translation="MEDRALNRVYTGRECRELDRIAIEEFGIPGFELMMRAGRAAFSE
                     LLARWPDASCLTILCGKGNNAGDGYVIGGLAREIGMAVQLVQLGDAADLAGDAARARD
                     WAVERGVLIEPGPERALRGDVLVDALLGTGIKGELKPAYREQVEAVNGSGISVLAVDV
                     PTGVDADTGAACEPSVRADVTVTFIARKLGLVTGPGASFCGNLVLAGLGVPDQVYDSV
                     AGTALLLHENLEALPARDANAYKQALGHLVVVGGDHTMGGAALMAGEAALRVGAGLVS
                     VVTRPAHRPAILARRPELMVVDADDDQSRREVLARATTIVLGPGLGRSAWGERLSREA
                     LALGKPAVIDADGLYWLATGALEPTAPVIITPHSGEAAMLLDSTSAAIQADRVTAVEH
                     LAEKVAGVAVLKGAGTLIAEHLGSTSRLAGVCGHGNPGMASAGMGDVLSGIIGGLLAQ
                     HDVLLSAAVRGVCLHSAAADVVARQVGQRSMLATDLLPEVVSMLAAAEQ"
     gene            52033..52452
                     /gene="tsaE"
                     /locus_tag="AC16QF_03885"
     CDS             52033..52452
                     /gene="tsaE"
                     /locus_tag="AC16QF_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR6447722.1"
                     /translation="MESVFLPDAEATEAFGRDLAVRLEPGDLVLLHGDLGAGKTTLVR
                     GILAGLGHSGSVKSPTYTLLEPYELTSCVVLHFDFYRIGDSRELEFIGFDELMDADAI
                     KLVEWPERAADRLPPPDLEITLRVEDEGRRIEVEAAR"
     gene            52430..53740
                     /locus_tag="AC16QF_03890"
     CDS             52430..53740
                     /locus_tag="AC16QF_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014819042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR6447723.1"
                     /translation="MKSKLLVSAFLLLCYAPPSSAGKLDRVRLHEAPDHTRVVFDVAA
                     PVEFSYFTLRNPDRVVIDLKDTTAGNDFDPSRTGTKSSRVKNIRAAPRGPDYRVVLDV
                     SAPLEPKAFALEPVAAYGHRLVVDLFGAAPKPAAPAAPKADDRRDVIVAIDAGHGGED
                     PGALGPGKVREKDVVLRIARKLEARLNATNGYKAFMVRDGDYYIALRKRVDLARRSRA
                     DLFVSIHADSFRSPEVSGASVYTLSSQGATSETARWMAEKENRSDLIGGVGGVSLDHD
                     DDLLNHVLLDLSTSAKQAASHEAAKSVLGQMARVTKLHKSRVEQAGFAVLKSPDVPSL
                     LVETGYISNPAEANRLASARFQDKIASALFEGVRSYMTRSAPPGTLLAWQRESGPVRY
                     TIERGDTLSDIAVRYGTSTRRLREANGIAGDRIRVGQVIVIPAS"
     gene            53764..55539
                     /gene="mutL"
                     /locus_tag="AC16QF_03895"
     CDS             53764..55539
                     /gene="mutL"
                     /locus_tag="AC16QF_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR6447724.1"
                     /translation="MAGRIQVLEPFVANQIAAGEVVERPASLVKELVENSLDAGARSV
                     RLETELGGMKRVLVSDDGEGIDAADLPLAVARHATSKISGPDDLVGIGSLGFRGEALA
                     SVASVARLTITSATAEAAHGTRLEVDGPGDVHIGPAAHPRGTTVEVRDLFFNTPARRK
                     FLKTERTEQSQVDQVLRRISLCRFDVAFELRHGGTRSVLSLPAGDPELRLARVIGEAF
                     VKRSVALDETRGELRLWGWVGLPTYSRSQADQQYFYVNGRVVRDKLVAHAVRQAYRDV
                     LFGGRQPVFSLYLELPADAVDVNVHPTKHEVRFREARSVHDFIFGTLARALREVRPDA
                     VAAPVAGEAAATGSPGSWTQGRQAGLGLPHAVQDRPFPPAPRSLQEAFAEHGAPITVT
                     ESDQPIPPLGYAVAQLHGVYILAQNADGLVVVDVHAAHERVTYEKLKRQMDTEGVPQQ
                     RLLVPVPVSVSARDADLLEQFEDVVSATGLSLDRSGTQSLMVRAVPALLAGANAEELV
                     RDVLADLAELGSSEQVRNRQHDLLATMACHGSVRANRELTLAEMNALLREMEVTDNAG
                     QCNHGRPTYMVQSMAELDRLFLRGQ"
     gene            55553..56527
                     /gene="miaA"
                     /locus_tag="AC16QF_03900"
     CDS             55553..56527
                     /gene="miaA"
                     /locus_tag="AC16QF_03900"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014860414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR6447725.1"
                     /translation="MTVPASGSVALVITGPTAVGKTELAIEIARRVPASLISMDSALV
                     YRGMDVGTAKPSPESLIEHPQALIDVRDPKEPYSAAEFVADADSLVQEAWAAGRVPVV
                     VGGTMMYLRAFREGLADLPQADPQLRRAIAAEAESRGWEALHEQLRAVDPAAAQVIHP
                     NNPQRLQRALEVHRLTGRPISDFWSEQRNADFAARLGGRMETIALMPDRRDRLHARIA
                     RRFAGMLERGLVEEVRQLRARGDLHADLPSMRAVGYRQVWQHLEGELDAAEMRERACA
                     ATRQLAKRQLTWLRGWSWVREVEWGDPVALAEQALLRVDQPHGSARRA"
     gene            56639..56887
                     /gene="hfq"
                     /locus_tag="AC16QF_03905"
     CDS             56639..56887
                     /gene="hfq"
                     /locus_tag="AC16QF_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HIR6447726.1"
                     /translation="MSKGHSLQDPYLNTLRKERIPVSVYLVNGIKLQGQIESFDQFVV
                     LLRNSVSQMVYKHAISTVVPSRNVRLSGPGGSEEAAEE"
     gene            56984..>57790
                     /gene="hflX"
                     /locus_tag="AC16QF_03910"
     CDS             56984..>57790
                     /gene="hflX"
                     /locus_tag="AC16QF_03910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR6447727.1"
                     /translation="MQVHLKSAAAPDAAVLAESELEAIELARSAGVDVCQVVSARRDN
                     PHPKWFVGPGKVAELAEVLASQEADLLIVNNELNAGQQRDLERDLLCRVLTRTELILH
                     IFADRARTYEGQLQVELAQLRHAQTRLVRGWTHLDRQKGGIGLRGAGETQIEMDQRAL
                     GEHIKRVQRKLEQVARRRDQNRRRRRRTRTRTVSLVGYTNAGKSTLFNALTRAGVLVE
                     DKLFATLDPTMRRLTVPGAGDVVLSDTVGFINQLPHDLVDAFKATLEEVSN"
BASE COUNT         9683 a        18528 c        19482 g        10097 t
ORIGIN      
        1 gaggcgcgcg gcggaagatt cccgccttcg tctacatgcc cgacgggccg gggccccatc
       61 cggtcgtcat ccacctgcat ggtggtccgc acagccagta ccggccgaag ttttccgcat
      121 cggcccagta ctacgtaaag gagcttggcc tggcggtggt tgcgcccaat ttccgggggt
      181 cgtcgggtta cgggaagaac tacctcaagc tggacgacgg ttatcttcgc gaggacgcgg
      241 tccgcgacgt aggtgctctg ctggactgga tcgcggccca gccgcgtctg gatgcggacc
      301 gcgtcgccgt cagcggcggc tcctacgggg gctacatggc actggcctcc ctgattcgat
      361 acggtgaccg gctggtcgcc ggcgtctccc gggtgggcat cagcaacttc gtcacgttcc
      421 tggagaacac gcgcgcctac cgtcgcgacc ttcggcgggt ggagtacgga gacgagcggg
      481 atccgcagat gcgcgcgttt ctcgaaggga tctctccgct cggccaggct gaccggatcg
      541 acagccccct gctcgtgatc cacggattca acgacccgcg agtgcccgca tcggagagcg
      601 agcagatcgc cgacgccgtg agcgccgcgg gtacgccggt ctggtatgtc ctcgccatgg
      661 acgaagggca cggattccgc aagaaggcca acagcgatta cgcggccgcg gttacggccc
      721 ttttcctgca gcgctacctg ctcgatgact gaagggccgg gctcacgagc tggggtggcc
      781 atctgcgtca atcccatgtc cggtcgagac gtcaggcgtc tggccgcgcg cgcaagcaac
      841 atgactcacg aggccaaacg cgacatggtg gcgcgggtgg cagccggcgc ccaggcgatg
      901 ggcgccagtg acatctacgt gacccgggag ccgtttcgaa tcgcgtcgtt ggctttggag
      961 cacatggggc tcgccgcggc cgtacacgtg cttcagacgc cgctgaccaa tggacctgcc
     1021 gacaccgaag cggcggtggc ggcttttctt gccgccggct gccgggtaat cgtctcgctg
     1081 ggtggggacg ggacgaaccg ggccatcgcc cgcgcccttg gggatgcggg cgcagcgggt
     1141 gcgggcgttg acctcatacc gctttccacg ggaaccaaca acgtctttcc cgttctggcg
     1201 gagcccactg ccgcgggtat ggtggcgggg ctgatagcct ctgggcggct gcccgacgac
     1261 gatctccggc agacggccaa ggtaattcat ttgtcgggca ccgatgcccg gggacgagcc
     1321 gttcgggacc ttgcgctggt ggacgcggcg ctgctgcgtg atgaccacgt gggcaacctc
     1381 ctgccgttcg ccgcgcagcg gctagctcga atcgtgctga cccgggccga gccggatgcc
     1441 gtgggcatgt cgcccatcgg ggggatgctc gatgtggtcg aggcggggga cgacgaaggc
     1501 ctcctggtgg agatgggtcc gggcagggag ttctcagcgc cgctctcgcc cggcgctttc
     1561 caggcggttt cggttcgctc ggcctcgcgt ctgccccttg atgtgacggt ccccctggca
     1621 gggcccggtg tgatcgcgct ggacggtgac cgggactaca agattgcccc ccgggaggcg
     1681 ctcacggccc gtatccgtcg ggatggcccc agggtcgtcg acatagccgc ggcaatgcgt
     1741 cgtgccgtcg ctcggggtat catggcgccc gcccgcaacg gttagaccca tgatcatcac
     1801 gccccgcatt cgtggattca tctgcaccac agcgcacccg gccggctgcg ccgcgcacgt
     1861 caaagagcag atcgattacg tctccgggca ggcagccctg gtcggtgggc ccgtcgtgcg
     1921 caatgccctg gtcatcggcg cctctggcgg atatggcctg gcctcgcgaa tcgtcgccgc
     1981 gttcggctgc ggcgccgcta cccttggcgt ttctctcgag aaaatgccga gcgagacgcg
     2041 caccgccagc gccggctggt acaacaacgt cgcgttcgag gaggcagcgg ctgcggccgg
     2101 gctctatgca aagaccctgg acggcgatgc gttttcggac gagatgaaag gccgcgtcat
     2161 cgacaccctc aaggccgaca tgggccccat cgacctgctc atctacagcc tcgcatcccc
     2221 cgttcggcag catccgcgta ccggcgagtt gcaccggtcg gtgatcaagc cgctgggcca
     2281 ggcgctgcac atgaagtccg ttcacgtgga ccgtggcgag gtggtcgagg tggacctgga
     2341 gcccgcggac ccggaggaga cggcggcgac ggttgcggtg atgggcggcg aggactggga
     2401 attctggatc gatgcgctga aggacgccgg gcttctggct ccggggttcc gcacgctggc
     2461 attcacctac atcggtacgg agctgacctg gccgatctac tgggaaggca ccctgggcaa
     2521 ggccaaagaa gatctggacc gagcggcggg cgccatctcc gaacgactgg aggatctcga
     2581 ggggaacgcc agggtggcgg tgctaaaggc cgtggtgacc caggccatga gcgcgattcc
     2641 ggtcgttccg ctctatgcgt ccctgctctt caaggtgatg aaggagcgcg gtgtgcacga
     2701 agacccgata cagcacattc accgtatgtt cgcgacccag ctctgcacgg gcggcgccct
     2761 gcgcctcgac gatgcggggc gcatacgcat ggatgacctg gagatgaccg atgcggtgca
     2821 ggaggacgtg cgcgaccgct gggaacgggt caccacagag aatctgtccg agcttggcga
     2881 tctggacggc tttcgcgagg agtttctgaa gattttcggg ttcggcatcg agggcgtgga
     2941 ttacgatgcc gacctcgacc ccaccctggg tcgcgccccc tgatacgcag cgggtcctcc
     3001 cgccacctgc caggaaactg acagcgcagc tggtcgatgt cgttggcgcg gagaatgcgg
     3061 tcgcgccaaa gtcgcgacca tggccctgcg aaaccttcac ctgcgcgcga ttcctttccg
     3121 ctacgcgggg tattcgggtc tcgcgctgac cattttggcc cttcagggca cgccgaggcc
     3181 ggcctgggcc ctcggggtcg gcgctgctgg ctgcctgatc tggaccatcg ccgcacatgg
     3241 ggcgggccgg ctcgcccaga ccaggggcac gtccactgtt ccattcgctc tgggcctgca
     3301 tctcgcagaa gcgctgctgg cgggcgcgct gatcggctgg ctgtccctgc cctggcttcc
     3361 cctgctggtg gcgatcaccg tgctgcttgc cagcaacgcc gctcaggccg gctggtggct
     3421 tgcctggcga gccggcatcg ccgtggcggt gggcataggc gcgggccgtc tcctggccgt
     3481 tgccccggtc atgagcagta cgccagcggc cgatctgaca gcctccgtcc tgctgcttgg
     3541 ctacaccgtc gggttgggtc tcaccagctt tgccaaagct cagcgcctgc acgccgctca
     3601 gcaggatctg gcaaggcggg ctctggtcac cgaacggctc aacagcaggc ttggccgtta
     3661 tctgccgccg ccggtgcgcg cccgggtgcg ccgccggccg gacgagcgct gcggcctcga
     3721 acgtcgctgg ctgaccattg cctacgtgga cgtcgtcggg ttcaccgagc tcgctggccg
     3781 gcttgcgccc gaggagctgg ccgtggtggt caacgactac ttcgcggcca gcggacggct
     3841 gttcgcctcg tccgggggca ccctggctgg tctgcagggg gacggtctgc tcgcgtactt
     3901 cggcaacgca accgcagcca cccggggtcg cgccgccgtg gattgcgtgg agtcctgctt
     3961 gcgggtgtcg cgcctcctgc gggagctgag cgagtgctgg cagcgtcgcg gctacgtcac
     4021 gaccctgacg acccgctgtg gggtggcaag cggctactgc acggtgggag actggggcga
     4081 agagcggctt gatttcaccg tcatcggcgc cccggtgaac ctggcaagca ggctgcaggc
     4141 ccacgccggc cacgacggca ttctgatctg cgagcccacc gctgcgctcg taggccaggt
     4201 cttccccctg ggcgcggagc gcgagctggt gctgaggggc atcgggaagg taaaggcgag
     4261 gaggctcgaa agcgcgctgg ttgacccccc gccggcgtct gctaaggtgc cgcgtccgga
     4321 agtgtgagca ccatgaccaa gacctttctt caggcctgca ggggcgaagc gacgcctcat
     4381 accccaatct ggttgaaccg ccaggcgggc cggtacatgc ccgagtacca tcaggtcaag
     4441 ggtgacatgc cgagcctgga ctttttcaag aatcctgaac gcgccgcccg ggcgacgctc
     4501 gatgctcagc gcatcctcgg cgtggacgcc gccattctgt ttgccgatct gctgcccatc
     4561 ctggagccca tgggattgcg tctggactat cagccgggcg tcggccccgt cttcgataac
     4621 ccgattcgat ccaccgccga cgtcgacggg ctcgtgagcc tgccggcgga agaggccacg
     4681 ccctacatcg cggagaccgt gacgaacatt ctcgaggagc tgccgggcga agtcgccctc
     4741 atcggcttcg cgggcgcgcc tttcacactc gcctcttacg ccatcgaggg ccgtggctcg
     4801 cgcaactacg tgttcgtcaa gaagctcatg tatgagcacg aggcggtctg gcatcgcctg
     4861 ctcgacaagc tggtcgagca ggtggcgagt tatctgcacc tgcagatccg tgctggcgtg
     4921 gacgcggtgc agctgttcga tacctgggtg gggtgtcttt ccgtggcgga ttttcgccgc
     4981 tacgtgctac cccacacccg gcggctgatc caggcgatca aggaaggatc gggtggccgg
     5041 gtccccgtga tctacttcgg aaccggcaat tcgcacctgc ttgccgacgt ggttggagcc
     5101 gggcccgatg tgctcgccct cgactggcgt gtccccctgg gggaaacctg gaaagcgctc
     5161 ggctgcactt cgatccaggg taacctcgat cccatcgtgc tctgcgcgga ccgcgagacg
     5221 gttgccgagc aggctcaggc cgttctcgac caagcggcgg ggcgcaacgg gcacatcttc
     5281 aacctcgggc acgggatcat tccggaaacg cccgtggaca acgtgaagtt tctggtggat
     5341 ttcgtgcatg agcgatcggc gcgtagcgcc tgagcgaatt gcggcggggc tgctgctggc
     5401 cgccttctgg ctgccgctgg cggtctgcac ctacctggcg ctgcacccga gcccgcccga
     5461 cgccgtgttc cgattgagcg acgtgctcct gcacggcttt gcgtttacct atctgaccct
     5521 tgccctcgcg ctggctcatg ggcatcggca ccgtcttttg cccgggctct ggatgctggg
     5581 ctacggcgtg ctgatcgagt tgctgcagag cttcgagacg tcccgttccg ccgaggtcgg
     5641 tgatgtgctc gtggacgtcg cgggaatcgg cctggggctt ctcgcatacg gatgggccgg
     5701 tgctttcctg cgcgcgctgg cggtgcgtat cgcgagcttg gtggtcggcg cgggcgcgtc
     5761 gaatgcagac tcctgacgcc agcgctcgtt cttggacgtc cgcgggagcc ggtctggcca
     5821 gctactcttg cccgagcacc tcttcgatga gcgccaggtg gctgccgagg aaaatgtccg
     5881 aaacggcccg gttggcgatg atgcggtccc ggccgatgag ataggtcact cggcgtactc
     5941 cgagcccgag ggggccgtcg acgccgtacg cgcggatgac cttcttgctg gtgtcgcaca
     6001 gcagagggaa tggcaggccg taggtttccg cgaagcgccg atgactcgcc tgattctgag
     6061 ggctgatccc cacgatcctg acgtccagat tgctcaggtc ctcgtggcgg tcacgaaacg
     6121 cgcaggcttc gcgggtgcag accgcagtga agtcggccgg atagaagtag agcaccacgg
     6181 gaccgctatc gagcagcgtg gcgagcttga tgggctcgcc ggcctggtcg gggagctcga
     6241 aatccggagc cgtatcacct ttgtcgagca tggggacccc tcgtcagcgt gccagctcga
     6301 aagagatcgg ctctcccagg aacccttcca aaggcagctc cccgaagttc acggccctgc
     6361 cgtggctcat ggcctcttcc agcgtgaggc cctcgtgcct caccagatag gctacccaga
     6421 ggtggctcgc ccgtcgccca gaagcacagt gcaggaggac cttgccctcg gtgccggcga
     6481 tcgcggcggc cagctggtcc actgctgagg gcgagtaggg gcttttaggc ccgcccgccg
     6541 ggatgtggac gtaacgcata cccagcgatt ctgcctttgc tttctcgtcg aatggtacct
     6601 gctgccggtt ggtcatctcg gccgcggtcc gcaggttgac gatggtgtcg acgccgagcg
     6661 ccctcatgcg ggtgagtgcc gaggcgtccg gctggccacc gatgtagacc tgcgccgtgc
     6721 gggccagcac gccgttgaag tcgccggcgg cctcgacgac ggggacaggg aactcggttg
     6781 cagccggcgg gggttcggca ccgacgccgg ggaagctcat cgcggtgatc agggcgagga
     6841 gcaggaggta tggcagtcgc attacggacg cgctcctgaa atttcgtcag ttgatgtcgt
     6901 cagtcttggg cggccgccgc tcacgtcgta cacgagctcg gcgccgtcag cgaatactac
     6961 cctcagaccg acgatcgtct gctccccttc gatgggctcg gccagcgaaa tcacggcgtc
     7021 gcggccaaag gcgaagggaa ggtccgtagt caggtcgcgc cagcggcgcg gctcggaagg
     7081 tgccggcccc gtcagaatcg cgcaacttcc cgttgcatca gggcgggcgt ggatcacgcg
     7141 cccgttcttg agctcgatct ccaaagaacc caacgatgag ggtgcggagg ggacggggac
     7201 gtcgcgaatg gaggccacct tgcccccgat cagagccgtg caggcgctgc ctgacgcctg
     7261 gctcaaagtt ttgtggcgtc caggctgacc accggctctt cccccagaac gcttgcacaa
     7321 tgcaccacgc cggccggcac gtgaaggatg tcgccggctt tgagcgtcac acgctcgccg
     7381 agcatctcca tgagaaactg tccggaaagc accccgtctt tcttgtcgcc ggcgtgtgtg
     7441 tgattgccga aacgggttcc cggaggatag gtgtacatgg ccacggcgta accgccgaca
     7501 gcgagcttgg cccgcatgtt gtcggggttc agctcaccgt cgatgtcctc ccgccagctc
     7561 tgcagatcca tcattgtcgc tcctgtcagg ggattatccg tcaacgccgc cggcggagat
     7621 accaccagcc cccgtagatc gcggcgttca cggccagcgc aagggcgccc agcagaacct
     7681 gggtcgtgcg ggtcatcgcc tgaggataaa gaagcggcag gaggtagtgg gcgacgaagt
     7741 caccttggta gccggcttcg ccggccaggg accgcagccg ggcttcccac ggagtcaggg
     7801 ggcacagcca gcccgcccac tcaaccacaa cggcccatgc aactgcgggc aggtgtgccc
     7861 acgcgagccg aggccagcgg atggtcagga agccaccgag aacgacgaac aggatgaaca
     7921 gcagatgcag cacgaccaac aggtcggcca acagcgcgta cagcgcagac aagatagcgc
     7981 tcaggcctgc ttcaggtcgg tctgaaccca gccgagccgg gtgtcggtat gtatctcgaa
     8041 cctgggtacg aacctctccg gctcgtccag gctgccgagc gtgacgtcca cgttgtccgg
     8101 gtggtcgtcg acgatgcagg tcatgggcgt gccgcagctc gagcagaaga accgccgccc
     8161 gcgatcggaa gattgcagct gtttcggtgt gcccgccgtg aatgagaact tttccttggg
     8221 caccacgagc cagctgacga acggcgcgcc gctggtgcga cgacacatgg tgcagtggca
     8281 gtgagccgcg gggtaatcac cgtcctcgat gcggtagcga acctcgccgc agaaacatcc
     8341 gccgtcgatc atcttcgcct cctagattcc cagcttgcca agagcgtcca ccagctcgcg
     8401 gaggaacccc tccacccggg gatgagagac cgccagcttc gcggccagcc actccagcct
     8461 gtcgcccagc ccctccagcg cgggcgtgga cgcctccagc acccgatgga tgtccgagtc
     8521 cacttccttg agcagcgaca cgagctcggg atcgatgggg tctgcgttct cgagctcggt
     8581 gtgaagcgcc tgcagcgctc tgcggatatc ctcgtcgctc atttgctgtc tcctcccgtt
     8641 gggttcgttc ggcggttgcc gggttggccg ctgaccccgt tcctgcgggc tggccgctgc
     8701 ggcaacgtcg gcccgttgcc atctcaagag ccaatctgcc aatagatcgc cctgaaatgc
     8761 aaacgcgctc cgccggcccg tcgcgccttt cgagtcgtgg tgacaagccg cgctttcgct
     8821 cctagtatcg tcgcgttgtc tgctgtttgg ggagcgaaaa tgttgcgctt cggaggagtc
     8881 tgttggctgc ttgccatcgc gttgctgctg ggcgcgtgcg accctcagga ccgccggccg
     8941 gggctgtggc tcaccggcga ggtggcagac gttgcgcccg gtgactgggc attttcggat
     9001 gagtatgccg agatagcgct ggaagtggcg acaccgtatc tgatcccaca ttcggtgacg
     9061 atctggtgtg cccaggtgga cggtaccctg tacgtcgccg cagcggcccc cgaggagaag
     9121 aactggccgg gctgggtatc cgatgaccct gacgtcaggc tgaagatcgg cgacaccgtc
     9181 tacgccgcga cccttgccga gctcgaggat gaggcggaga tcgcagccgt tcgcagcgcc
     9241 tattccggaa agtacgaagt ggcgaacctg ggctcgggcg cgtcgctgcg ttattggcgg
     9301 gttggcccgc gaggcgcgtg accggggcaa ctgctcgagc gggtcagttg cgccccggac
     9361 aggccagccg atcaaagggc caggcctcct ccagccactg cttcacagca gagaaaaccg
     9421 cctcgtccat gtctgagtgg ctggtgcgca cccacaggta gacggaggca tcgaatccgt
     9481 ccggcgcctc gggataaacg tagaagcagc cgatggtcag ggtgtcgtcc gggtcgacga
     9541 gcgtgtaggc gaacgagcgg cgcttctgga actccttctg gtgccagccg aggtcgatga
     9601 gattctgctt gagggaaaga tcggcggcgg gccaactcga ttccgagccg aacgtgccga
     9661 ccagccgttc acggctgctc atcaccgcgt cgtagtcgcg aaccacgtcg tccacggtca
     9721 gcggtctggc gctgaaggtg ggcgtgacca gaagttccgg aacgtgaaaa cccgccggca
     9781 gaaagctcac gagactatga tcccctcaga ggctccaggg cgcacacccc gataacacag
     9841 atcggccggg tttcagggca cgaccgctcg cattctacct gctcatgact gccgcacagc
     9901 gcggccagcc gatcgcggtg ctcggcggca aacgactcgt tgatggcggt gccgcaatcg
     9961 cagccgccgc agcgggtctg gaccttggtg cattggcgtg ccgcctggca gtagcgttgg
    10021 gattcgctga cgcgaatgcc ggcagccgat cggtcaccgg tcaccacggg tgagaccgcg
    10081 agcagcgcag cggcgatgat gattgcaacg gccctcatgg cgggctcaca gcctgatcag
    10141 gaaaggcgcc ccaccgacga cggccccggc gaccagaagc gttgacgtgg agccgacgaa
    10201 aaatgggatc gaaacgagcc ccagaccggc aatgaaaaaa acgggcgcct gctggcggcg
    10261 tccgtatacc agatagccta tgccgatggc gctgagcacc aggcccaaga gaaggtgaat
    10321 ttcgttcatc cgctcctcag cgattgctgg cgggaggact ataacagcgt gctgaacacc
    10381 tttgcagacg ccccgggatc gatgcctgaa gctaacggta gacggcctct ttgccgtggc
    10441 gtatctccag ccccgttctc tctgcttttc tgaggcctcg gacgaccaga gcgtatgatc
    10501 ggtccatcat gcgctccagc tcgcccgcag gaatggatcc gtcgagcgtg accgtgttcc
    10561 agtgggcctt gttcatgtga taaccgggcc gaacggcctc gaagatgtcc cgcagggcca
    10621 ttgcctcgtt gggttcgcat ttcaggttca tctgggcgac gccgttcacg tagctcaggg
    10681 ttgcgaacat cttagggccg atcttgtaga cgtagacatc cggtccgaac gggaagtctt
    10741 cccgcgcctc gggccttgcc aggaggtggg cgcgcgcggc ttccccgtcc atgggcggat
    10801 gctctgcggt cactcgtcgt cgtaggattt gaacaggcag ctgcgggcga attccgcggc
    10861 ctcgttggca gtccaatcgc ccttgtcctt ctcggccatg tcttcgcacc acgcgtcact
    10921 tcccacctcg ggcgagcaag ctgcgagcgc tgcggcgaga acgatgatcg atagcttcgt
    10981 ttgaatgtgc attgtgctct ccggcccagc gggcgctcgg ggtttcaagg atcgttcagc
    11041 tggctcgtgg ggcgccgttg ctagggacgc tgcggcgacc gtggcaagcg gcccgcgagg
    11101 cccccgcaaa tgaccacctg gcgggtttcc tgcgccccgg cagcctgttg tatatgcagg
    11161 aaaaacgagt tactttctcg tcaccggtcg ggcctcgagt ttaacgcctc tctgagctgc
    11221 cttcgataag tctaattcag caagcggtgc gggtgcctga tagtgcagat tgcgcccgcc
    11281 ggtctggggg atgagaacgt accgcttgcg cactaaggtg aatatgaagc tatttgttgg
    11341 aaatctgccc tggagcgtaa cagaccagga tctcgaagaa atgttcgcgc cgttcggtgc
    11401 cgttggctct gccaaggtga tcaccgaccg cgacactggt cgatcgcgtg gtttcggttt
    11461 cgtcgagatg agtgacgatt cggcgcgcaa cgccatcgca gatctgaacg agtccgagat
    11521 atcgggccgc aagctcacgg tcaacgaagc ccgcgatcgc gatcgcgggc gccggtagcc
    11581 gcaccggaac acgtcagcct gtcagtcaaa cgcgcgacgg gtccctttag ggccgtcgcc
    11641 gttggcggtg ggctggcttc agttgacagg cacgtcggcc gcccgatggc cgtggcggag
    11701 gctcgtcatc ggcacgcgac gatcatacga cgagtgacct gcgagccttc ggtcgaccaa
    11761 tcgttgcggc cgacgttcgc gctcccctcg ttgctcacct gggagaccac gtcgaagccg
    11821 cgcccggcgc aggcctcggt ggccagcgcc tggcagcgcg tccacgacgg ggtgccgccg
    11881 gagcaatcca ccgagatgcg cccgtcgccg agatcttcca cgcgggggtc cgacgcgcag
    11941 gcgctgagcc cgatcgcggc cgagacgatc agggcgcgac cacggatggc cctcatgcct
    12001 gttcctgagc ctgcgaagcc ttcttgacca gaagaaacca ccaaaagatg cctgacaaga
    12061 ttccaaacgc gcctacggac aggcacacga ccagcagcgt ctgcgtcgat ccgctcagcg
    12121 tggcgaagag gtagccgaac cccaggccgg cgacgaagcc gccgctggcg aacgcgtaaa
    12181 ccgagagcca ctttcttgcc ttgagaaaga agaggacggc gagcagcacg gcctggaaac
    12241 ccagcagaaa tatgggtacc agaacgaata cgaaatagtt gaacgcgtcg atcacgtccg
    12301 gtctccagtc tttgcttgtc ggaaggctac acgggccggg cccctacgaa ccttgcccgt
    12361 tcccgatgtt cctgcccgaa ggcgccgtca gcgtttgcac ggcgacgaag gcaacgtaac
    12421 agaaaatcgc caggttgagc agggcgaccc acagggcgga gtcgtccctg acgtaggccc
    12481 ggaacgcgcc gataggaatg acgacggcaa acgcgagagc gatgacggcg attcgccgag
    12541 gttgggttcg gctgaaaatg ctggtgacga acatcacgtt ggcaaacagc acgggaccga
    12601 ggtggatgac cgacgagacc aggtactcca tgccggagat cagcagcagc agtatggcgg
    12661 cggctttcag ggtgagatcg agattcaaga tgaaggatcc tcgaggccgt cgatcgggcg
    12721 ccgtccgggt tgaggggcga gctcggctga tgacgtggcg gcgcggttca tctgcagtgc
    12781 gtcgagtccg gcggggcgcg tcgtcagggc cgaatgacga tgggggtacc gatctcgacc
    12841 agggcccaga tctcctctat ctcgctgtcg gtcaccgcga cgcagccggc ggtccagtcg
    12901 atgtaggggt gcagtttgcg catttccgga tcggcctcgc cgatgccgtg gatcatgatc
    12961 tgaccaccgg gattgactcc ctccatcgct gctcgccgcc ggtcggcctc gttcgggtag
    13021 gagatccgca gagagcggtg gaatgcgctg tcctcgttgc gggcatcgat ggtgtattgc
    13081 ccctccggcg tgcgctggtc gccctcgcgc tgcttgtgcc cccgcggcgc cccgcccagc
    13141 gcgatcgagt aggatcgcac gggtgccccg tcgtgctcga ggatcagcac tcgctgagac
    13201 ttctcgatca ccagccggtc aatcttgaga tccgcaagcg ctgccggtgc cgacccgagc
    13261 agcgggccca gcgccagaag cgccagtcgg gctttaaagc gaacggaaaa gatgtcgaaa
    13321 gggatcgagg aaatttgcct catgcccgta ccatgaccgc gtgacctctc gagcccgaag
    13381 atagcacgtg aattctcacc tgcaacggtc agtttgtatc cgggttacgg ctcgggtggc
    13441 ggcacctgat cgaaggttat ccgaaccagc atgtggtctt cagggtcggc gatgacctcg
    13501 agcgcctgca aggcatcccg ataggctcgc gcgtgggcag tgtaatcccc ggctacgtaa
    13561 gcagcgtggg cgtagatcgc gtgggcgaag gcgatctccc aatcgggcgt gtcccggtcc
    13621 agaaagaagg tctgcatttc ctcggcgtac cgcagcgcgg aggggttctt gcccagaagc
    13681 gcgtgcacct tggcgagcag cattgtggcc cgcatcctgt tgagctcctc gccgacttga
    13741 tcccagtgaa aggccgcggc atgcgcggct tgcagcatct gctcatcctc aggcgccgtg
    13801 cgggcgcggc cggccaggtc ccaagccgcg ttgttgcact cgatggcaaa gtatcgatgc
    13861 catgatgcga tgtcgggctc ccgcggatgt tgcggcacgt ctgcttatcc ccgtttcacg
    13921 gcatgctgcc gatgggctca atccaggttc ttgtcggtaa cggcgccttc actcgccgag
    13981 ctgaccagcc gggcgaactt cgccagaatg cctttcgtgg cgtacggcgc cggtttctgc
    14041 cagcgggcgc ggcgcgcggc caggtccgcg tcgctcaact ggacgttgat ctcacgcttc
    14101 tcggcatcga cggtgatcag atcgccgtcc tcgatcagcg cgatgggccc tccctcgtaa
    14161 gcctcgggcg tgatgtgacc gatgacgaag ccgtgggagc cgccggagaa acgcccgtcg
    14221 gtgatcagcg ccacgtcctg actaagaccg cggccattga ttgcgctggt gggcgagagc
    14281 atctcgcgca tccccgggcc gcccttcggc ccttcgtagc gtacgaccac cacgtcgccc
    14341 ttgttgatgg ccccgtccat gatggcggcc atggcggcct cctcgccgtg aaagcacttc
    14401 gcgctgcccg taaaggacaa cccctcgtgt ccggtgatct tcgccacggc cccggtgggg
    14461 gccaggttgc cgtagagaac gaccaggtgg gaatccttct tgatcgggtc gctcatggga
    14521 cggatgacgt tctggccatc cggatacggg gccacgtcag cgaggttctc ggcgagggtc
    14581 tttccggtga ccgtgaggca gtcgccgtgc agcaggccct cttcgagcag cgttttcatg
    14641 agcggctgga tgccgccgat ggcaatgagc tcactcatgg cgtactggcc ggccggacgc
    14701 atgtcggcca gcacgggaac cctctcgccg acgcgggaga aatcgtccag ctccaggggc
    14761 acgccggctg cgtgggcgat ggcgagaagg tgcagaacgg cgttggtcga tccttccagg
    14821 gcgatggcga cggtgatggc gttctcgaac gcctcgcggc agagaatgtc gctgggcttg
    14881 atcccgcgct cgatcaggtt gaacaccgcc gcgccagcct ggtagctgtc ctggcgcttc
    14941 gcattcgaga tcgcttcctg ggctgagctg ttcgccagcg acaaacccat agcctcgatg
    15001 gcggatgcca tggtgttcgc cgtgtacatg ccgccgcagg aaccgggccc cgggatcgcc
    15061 gtggcctcga cttccttgag ctcaatatcg gagatccgtc ccgccgcgtg agcgcccacg
    15121 gcctcgaata ccgagacgat gtcgcgtctt tcggcgccgg gctggatggt cccgccgtag
    15181 acgaacacgc tcggacggtt caggcgggcc atggccatcg cgcaggcggg catgttcttg
    15241 tcacaaccgc cgatggcgac gaacccgtcg aatccctggg cgccgacgac ggtctcgatg
    15301 gagtcggcga tcacctcccg cgaaaccagc gagtaacgca tacccggcgt gcccatgctg
    15361 atgccgtcgg aaacggtgat cgtgttgaac accacgctct ttccgccggc ttcgtcggcg
    15421 ccgagcgctg ccgcctcggc cagggcgtcg atgtgcatgt tgcacggagt gaccatgctc
    15481 cacgtggagg cgatcccgat ctgtggcttt gcgaagtccg cctccgtgaa acccacgggg
    15541 tagagcatcg cgcggctcgg agcctgctgc acgccgtcca cgacgatgga cgagtagggt
    15601 cttttctggt cagtcatgga gttcccctgg ggcccggcct cactgccggt tacctgagtt
    15661 gcttgatcat caccttcgag ttgcgctggt agttatacat agcctgcttg ggcgctggaa
    15721 gcgcgtcgag agtggaatcc tcgaagccgt tgtccacgaa ccagtctcgg gtctgggtgg
    15781 tcagcacgaa gagcttcctc aggcccatcg cgcccgctgc ggcttccgtc gcccttaaga
    15841 gcgcaacccc gaggcgggtc tctccaggct tgctgcgatg ggattcgtgg acggccacgc
    15901 acgcgagctc cccgcagtcc tcgaagggat acagagcgca gcaacccatc acgatgccgt
    15961 ccacctcggc aacgaagaaa cgatcgatct cctgctccag cctgtcacgc ggacgcctta
    16021 ccagcacgcc ggcctcctcc atgggcctga ttagctcgac gatgccggcc acgtcttcga
    16081 gccgcgcctg ccgcacgagg ttcgagtgat cttcgctgat ctgggtgccg cctccgccgg
    16141 ccgtgaagag ctcctccagc agcgcgccgt catgcgcgta gctcaccagg tggcaacgcg
    16201 gcactccgcc tcgacaggcg cgcagaagcg ctcgcagatg accgcgggtg gtcgtgtcat
    16261 ccagggccgg aacgtcgatc agggtatcga gctgggcggg tgtcaggttg ggttgcggct
    16321 cgccgcgttc gttgctgacg tagcgatcgc tgccaaaggc gatcagcttg tcggcatcca
    16381 gggccatggc cacgtctgcc gcgagctcgt ccgcggccag gttgaacgcc tgcccggaag
    16441 gcgagtagcc caggggagac aacagcacga gcgcgccggt atccagggct ttctggattc
    16501 gccccccgtg gactcgtcgc ggccggccgg tgaacaggtg gtccacgccg tcatggatgc
    16561 cgacgggtcg cgccgtgatg aagttgccac cgaccacgga gatgttcgtg ttgtgcagtg
    16621 ggctggtggg cagcccggtg gagaagaggg cctccagtcg ggcgcgctgc tgtcccagga
    16681 tgccgatgat cgtctccatg gcggctgcgt cggtgatgcg gcgatcctcg tgaaagggcg
    16741 attccccgag ggccgcatcg atctgcggcc gtgcgccgtg caccactacc acccgtgcac
    16801 ccaggacgtg cagcagcgcg agatcgtgaa cgatgttcgg caggtttcca tgggcgacgg
    16861 cgtcgccccc gagaaggatg acgaacgttc ggttgcggtg cgcgctgatg tagggggtcg
    16921 acgccctgaa ccatcgtgcg tatccgttct ctgcttctgc cttggccggt ctgtcgctca
    16981 tgaattgctg ccctgccgcc gcccccctcg ggaagcgcgt cgtcaaaaaa gggggcgcat
    17041 tttacaccag cgacctcgaa tggcatggca aacgtgacgc cggtctaatt ccgaaaatgc
    17101 catcgccggc tctgggcgct agccggttgc aaaagccgca tccttgtcat taactgaaac
    17161 ttcttggcta atatcaggga cttgcgtggc caaccccctc attgattctc cggcgggcag
    17221 ggatccgcgt ccgctgtcgg agggagctgc tccgttggca gccgggtcgg agccgaaggt
    17281 cgcctccctt gccagccgcc tgccgttggc gcaggagcag ctcatcgacc tgccggtgct
    17341 tctcgacggg ctcgtcaagg aaggctacat cagccagcgt caggcggaag atatcctgat
    17401 cacgccgcgc accaagaaag agctcagcca gcatcccatg gagatcgttg cggatcgcga
    17461 tttcgacaac cgtaacaagc ctggccacaa actcgacctc gagaccatga cggtctggct
    17521 ggctcatcaa agcggtcagc cctatctgcg catagatccg ttggacatca atgtcggcgc
    17581 ggtcacggac gtgatgtcgt acgcctttgc gcaacggcac aacatcctcg ccgtggacgt
    17641 gaccgacgac gaagtcgtca tagccagcgc ccagccctac atgtaccagt gggagaccat
    17701 gctcacccag accctgcggg gccggcgcat cagacgggtg gtgactaacc ccgcggactt
    17761 gagccgctac atgctcgagt tctacaccat ggcccgctcg gtggcgaaag ctcaggatgc
    17821 ggggatggag atgagcggcc tggcgaactt cgagcagatg ctcgagctcg gccagatgaa
    17881 gtctccggaa gccaatgatt cgcacatcgt caacatcgtc gactggcttc ttcagtacgc
    17941 gttcgaccag cgggccagcg acattcacat cgagccgcgt cgcgacaagg gactgatccg
    18001 ttttcgcatc gacggcgttc tgcaccacgt ctacgagttg ccgcagcagg tgaacgcggc
    18061 agtcaccagc cggttgaaga ttctgggccg aatggacgtg gctgagaaac ggcgccctca
    18121 ggacggtcgc gtaaagacct tgagcccgga tggcgacgag atcgagctgc gcctgtcgac
    18181 cctgcccacg gcgttcggcg aaaagatggt gctccgtatc tttgatccgg acgtgctgct
    18241 gcgcagcttc aaggagctcg ggctgtccgg cgacgattac gagcggtggc acggcatgac
    18301 ccactctgct cacggcatcg tcctggtcac gggcccgacc ggctcgggga agacgactac
    18361 cctgtactcg acgttgaagc agctggccac ttccgaggtc aacgtctgca ccatcgagga
    18421 ccccatcgag atggtcgagc ccgccttcaa ccagatgcag gtgcagtcga acatcaacgt
    18481 caccttcgcc gccggcgtgc gcgcccttct ccggcaggac ccggacatca tcatgatcgg
    18541 tgagattcgt gatctcgaga cggcagagat ggcgattcag gcggctctca ccggccacct
    18601 cgtcatatcc acccttcaca ccaacgacgc cccaaccgcg atcacccgtc tgctcgatct
    18661 cggggttccc ccttacatga tcaaggccac cgttctcggt atcatggccc agcgtctggt
    18721 gcgtacgctc tgtcctcact gcaaggtcga agaagagacc gatgcggagg cttggcggct
    18781 tctgaccaag ccgttccgcg caaagcagcc ggagcgggtg ttcgcaccca aggggtgtct
    18841 ggagtgccgg gacacggggt acatgggacg ggtgggcatc tatgagctgt tgccgatgag
    18901 cgagaaggtc cagaccctgg ttcaggcaga cacggatatg gatgttctgc gcaaacaggc
    18961 tttcaaggac ggcatgttga cgctccgcct gtcgggtgcg cagaaggtcg gctctggcct
    19021 gaccacgatt tcggaggtcc tgcgggtggc tccggcctcc caagactgaa catttgtcct
    19081 cttcttcggt acccccagaa ctgctgagca aggccgagcg aacggctgcc cgacttccgt
    19141 attcccttcc tgccgacggg ctgcgggtgc tcgcgttgag cgacttcgcc gccaacgtga
    19201 tggcgcggga ggcccaatgg attcaggacg cgctcgaggc cgaccgcttc tccgcccccc
    19261 tcgcgtcggg ggaagtccgc gcgacgctcg ccgccgtttc ccaaggggcc gaggacctag
    19321 gcgccctgca gcagagcctg cggcagtggc gcaaccgcta tcagctctgg gtggtctggc
    19381 gccacctcct gcggatggcg ccgctggaag aaacctgcgc caccctctcc atgctggcag
    19441 acaccatgct ggatctcgcc ctggagcgtc tttaccagtg ggccacggac aggcaaggcg
    19501 tgccgatggg ggagtcgagc cgatcgcccc agcgcctggt ggtgctggcg ttggggaaac
    19561 tcggtgcccg tgagctgaat ctctcgtcgg atatcgatct ggtattcgcc tacccggagc
    19621 ccggcgccac gacgagcggg accaccaacc agcagttctt cctccgcctg ggtcagcagc
    19681 tcattcaggc gctggatgcg gtgacggcgg acggctttgc ctttcgcgtc gacatgaggc
    19741 tccggccatt tggcgccagc gggcccctcg tcatgcactt cgagggcatg gaggactact
    19801 tcgccgctca tggcagggac tgggagcgct atgcctggat caaggcgcga gcctgcgcag
    19861 gggacgtagc gtcgggagag gccctgcttc agtccctgcg gcccttcgtg tacaggcggt
    19921 atctcgattt cggggcagta gacgcgctgc gcgacatgaa gcgcaggctc tactccgagc
    19981 gtaacgatcc caatgacgtc aagctcgggc ccggcgggat cagggatgtg gagttcgtcg
    20041 ttcagctgca gcagatggtc tggggcggga ggcagccgga gctgcaggaa gccaacctgc
    20101 tgcgtgtcct gccgcggctc gcctcccagg gccatctgag cgacgcggac gcggccgcgc
    20161 tggccgaagg ctatcgattc ctgcgtgaca ccgagcacag catccaggcc gagggcgacc
    20221 ttcagaccca gtcgctgccg gcgtccgaag acagccgcca gcggcttgcc agctccaggg
    20281 gctttgccga ctacggcgcg ttcgaaagcg cgctgaacgg gcaccggatc gcggttgaag
    20341 ccgtcttcag gaacgcggtg ggggagctgc cggaggaaga cacgcccggc cagcgcctct
    20401 ggtcaagccc ggatgacgcc gcgctgctcg gcgaattggg tttcgcgcga gccgaggcgg
    20461 cgggcaagct gctgaccagc ctgatggaag cgcgcgatcg gccctcccag agcgccgaaa
    20521 gcagagcccg gctggaccgg ctcatgcccg gccttctcga ccgggtgagc cttacggagg
    20581 atccggatct ctcgcttgcc cgcgtggtgc ccgtgttgcg atccgtgctg cggcggtcgg
    20641 cctatctgtc gctgctccgc gaaaaccccc acacccttgc tcacttcgtc gacctgacgg
    20701 ccaaaagctt gtggttggcc gagcagctcg cccgctttcc agcgttcttc gacgcgctgc
    20761 tggacgaacg ccagctcgct gcgcttccgg acaaggccgc ccttcaggcc gagctgcacc
    20821 agcaggtcga ggcggcgccg gcggatgact tcgaacgtcg gctcgacgtg ctcagggagt
    20881 tcaaggagca tcacgtgttc aatgtctccc tggcggaggt tcgtggaacg ctgcccctga
    20941 tgaaggcgag cgactacctc acgtttctgg ccgaggccgt gctcggcgag tcgctggacc
    21001 tggcctggaa cgaaaacgcc gaccgctttc ccgagttcgc cgagcctcga cctttcatca
    21061 tcgtcggtta cggcaagctc ggcggtatcg agctcgggcc gggaagcgac ctggacctgg
    21121 tcttcattca cgacctgccc gtcggcgcct cgcagttcct gcaccggatg gtccgccgcc
    21181 tgcttcacat cctcaccgtt cccacctatt ccgggacgct ttacgagatc gacatgcgcc
    21241 tgcgcccttc gggcagttcg ggaaccatgg tttcgagcct ggaggctttc gccgagtacc
    21301 agcgatccca ggcctggatg tgggagcatc aggcgctggt tcgggcgcgc gcagtggctg
    21361 gcgatccggc tctcgcccag cgcttcgagg cggttcgacg ggaacttctg tgcgagcccc
    21421 gggaccgcgg gccgctgcgc gaggaagtgc tgaagatgcg agcccgcatg gccgagcacc
    21481 atggtgacgc ggaagacctc aagcgaggct ccggcggtat cgtcgatatc gaatttatgg
    21541 tgcaatacct ggtcctggcc tgggcgcacg aacaccctgc gctcgctgaa ttctcagaca
    21601 acgtgcgcat tctagagacg gctgagcggc tggatctgct tcccgcaaac atggcccgga
    21661 acctccggga ggcttacgtg ggtctgcgct ctgagtggca ccggtcggtg cttgacctgc
    21721 cggacagcga gcgggctgcc cggaccctga tggcccacag gtccgacgtg agggatgcgt
    21781 ggcgcgaggt gtttggtatc gacccggaaa acgagtaagc gcggaatcat gacagaaccg
    21841 acattttcgg accgagacgg ctggatctgg cacaacgggg aactggtgcc ctggcgtgac
    21901 gcgaaagtcc acgtgctgac tcacacgctg cactacggcg tgggagtgtt cgaaggcgtg
    21961 cgggcgtatc agaccccctc gggccccgct attttcaggc ttcacgagca tactcgccgg
    22021 cttttcaact cggcgcacat cctcggcctc aaaatcccct tcagcgtcga cgaggtcaac
    22081 gcagcccagg tcgacgtttt ccgtgccaac gctctgaacg aaggctacct gcggcccatg
    22141 gtgtacctgg gttcagaggc catgggcctg cgggccaagg atctttcggt aaatctctgt
    22201 gtcgccgcct gggcgtggcc ggattacatg gaccccgaca gccgcgagaa ggggatcaac
    22261 gtgcggacgt cgtccttcac ccgccaccac gtgaacatca ccatgtgcaa ggcgaaagcc
    22321 aacggcaact acatcaactc cattctcgct cttcatgagg cgctggacag cggatgcgac
    22381 gaggcgctgc tgctcgacaa cgagggatac gtcgccgagg gcagcggcga gaacgtcttc
    22441 atcgtcaagg atggcgaggt gcacacgccg gagcttacct cctgtctcga cggcatcacg
    22501 cgaaatacga tcttccatct ggccgcggag atgggcatcg ccatcaggga gcggcgcatc
    22561 accagagacg aggtgtacat cgccgacgag gcgttcttca cggggacggc cgccgaggtg
    22621 ctgccgattc gcgagctcga tggtcgaatg atcggctcgg gcaaacgcgg gcccgtcacg
    22681 gagcggttcc agtcggccta cttcgaccag gtgcggggca aacgcagcgc cttcccggag
    22741 tggcacacgc ccgtaagcgg tgcccaatag cggcacccgc atcctgatcg tcgcccccgc
    22801 ttgggttggc gacatggtca tggcgcacag cctcgtgcag gtcctcatcg cgcggcacgg
    22861 cgaccccggg atcgatatgc tggcccctcg cgccacggcc gggctcggag cccgcatgcc
    22921 gggcgtgagg aacgttcacg tgctcgacgt ggagcacggt gagctcgccc tgggcgccag
    22981 gcgcagctcg gggctgaccc tcaaggatca gtacgaccag gccatcgtgc tgccgaacag
    23041 cttcaagtcg gcgctcgcac ccgcgtgggc caggattccc gttcgcaccg gctggagcgg
    23101 cgaagcacgg gtcggggtgc tcaacgaccg gcgccgcctg aacccggagc gctacccgag
    23161 aatggtcgac cggttcgcgg ctctgggact cgatcccgac gcgccgctgc cggacgatat
    23221 tcccggtccc gtcctcgagc ccgatgccgc tgccgcggag cgcctgctcg cctcccgccg
    23281 gctctcggtg gacgggggcg tgaccgccct gtgcccgggg gccgagttcg gcccggccaa
    23341 gcgctggccg tccgttcact ttgcccaggt cgccagctac gccgccgctc aaggacatca
    23401 ggtatggctc gtggggtcgc cgaaagatgc cggggtatgc tcggaggtgg aggcgcagtc
    23461 gccggccggc gtggtcaacc tcgcggggcg caccacgctg ctggaagccg ttgacctgct
    23521 ctccagggcc gaccgggtcg tcagcaacga ttcgggtctc atgcacgttg cctcggcgct
    23581 cggccggcct gtggttggcg tcttcggatc aacctccacg accttcacgc cgccgctcgg
    23641 cgagcgagcg acgacggtga gcctgggtat cgagtgcagc ccgtgctttc aacgcgaatg
    23701 cccgctgggg catctgcgct gcctcaacga cctgccgcct caacgagtca tcgagctgct
    23761 ctagtgcacg tcctgctgat caagatgtcg tcgatggggg acgtggtcca tgctctgccc
    23821 gcggtgacgg acgccgcgag ccatggggtg actttcgact gggtagttga ggaggcgttt
    23881 gcggacatac ccgcgctgca cccggcagtc ggccaggtga tgcccattgc ctggcggcga
    23941 tggcgctcct cgctgcgctc gtccgggggg gagctggccc ggttctggga gcagctccgt
    24001 cgccggcgct acgacctcgt cctcgattct caggggctcg tcaaaagcgg cgtggtggcg
    24061 gcgctggcaa gaggaagaac caaggccggg ttctctcacg cctcggctcg ggaaagggcc
    24121 gccgccctgg cttatcggcg cggggttcgg gtggcgcgca accagcatgc catcgaccgc
    24181 caacgcgagc tcttcgccgc ggtgttcggc tattgcgtcc ctgccgggct cgactacgga
    24241 atcgcgcggc cggcgcatca ggccagcaag accaaccatt gcgtgctgct gcacggaacc
    24301 acctgggaga gcaagcacta cccggagcat ctttggcggg aagtcgcccg gcgcgctcgc
    24361 gcggccgggc tggacgtagc tctgccctgg ggctccgagg cggagcgtgc gcgggcggaa
    24421 gcgatcgcga cgacgaacgc cgacgtcctg ccggccctga accttgccga gctgaccacg
    24481 cgaatcgccg cggctcggct cgtcgtgggc gtggactcgg gactcgctca cctcagcgcg
    24541 gccctcgacg tgcctacggt ggtgatctac ggcagcacgg acagcgcact cacgggatgc
    24601 cgcggccgca gggtgagcaa tttgcaggcg cagtttccgt gcgcgccctg cctgcgcaaa
    24661 gcttgcggct atcacggccc ggaggaaatt ctgcatcagg cgccggtgcg gcccgcgtgc
    24721 tacagccgcg ttacgcccga tgcggtgtgg gatgccgcag cccgagccat ggcggatggg
    24781 ggtcgggaga ccgccccaaa cccggggtcc gacctgtgac ggccacgggc cggccgcttt
    24841 ctttggcgga gctgcaggcg cagggacgcg atctgccgac gccgttcatt ctggaaggat
    24901 ccctcaatcc ggcccctgac gatgggccga tcgagtgtct cgaggtcctt cgcgtgctcc
    24961 cgggccggcg aatcgtcatg cgagcgcggc tgggggctcg tcaatgcgcg ctcaagctct
    25021 ttgttgggga ccgggctgcg ctttccgcca ggcgggagcg agccggcctg gaattgctgg
    25081 cggccagcgg ggtggacacg ccggagctga ttgccgacct ccatctcccc ggcggcgggg
    25141 agggcatcct ggtggattgg ctggcgggca gccggccggt ggacgaggaa gatgtccaag
    25201 gcctggcgga tgttgcggcc gtgcttgcac gcctgcacgc caacggtgcc atccagcacg
    25261 acgctcatct gaacaactac ctccgcgatg cagacggcat gctgcatgcc atcgacggcg
    25321 acggcgttcg ccggcctgca cgtcttcggc gcagacagga actcgccaac ctcgccctgc
    25381 tgctggccca gctgccgccg ccggatgcga ctcgcctccc ggtggtggcg gaagcttact
    25441 ggcgtgcgcg ctggggatcg gaccccggcc cggacgagct ggctgcgctg cagcgacggg
    25501 tgcacgctca gcgagccggc aggatgcgcc gttacctccg gaagacgctg cgggattgca
    25561 gcgagtttca cggcgagcgg aaaaatggcc gcttcgtggt ctgtgcccga aatgcctgga
    25621 ccgcgcaaat ggccgagttc gtcgaccgtc cggaagacca cctggtggcc ggcaccgtgc
    25681 tcaaggcagg caacagcgcc acggtgatgc gcctgcgaat cggcgaaacg gcgtacgtgg
    25741 tcaagcgcta caacgtcaaa ggtttctttc acgggctgcg gcgggcgctg cgccccacgc
    25801 cgcgctatcg cgtggcgtgg ttgagcggtc atcgcctgct tcgcctgggt attgccacgc
    25861 cccggccggt cgctctgctg gaagcgcgcc aggggcttgc taccgtggcc tatcttgtca
    25921 tggaggatct cggtgcggcg cgggatctgg cgcaggccgt cgccgaggaa ggatacaccg
    25981 cgggtgtcgg acgtgcggtt gccgggttgt tccgcgggct gcgcgacgcg ggcctggtgc
    26041 acggggatac gaaagccagc aacttcctgg tgcgcgacgc aagcgtctac ctcatcgacc
    26101 tggatgccat gaagccgggc agccagacgc ggtcccgcga cgtcgcccgt ttcctggcga
    26161 actgggacga tgcgccacag gtgtgcgagt cttttcgcgg cgccttgcag gaggccggcg
    26221 tgccgctata gtacgcgcac tttccgatcc caggtttgcc gttgatcatc ctcggccttt
    26281 ccggagcgct caaccacgac ccttcggccg cgttgcttgt cgacggggag atcgtcgcgg
    26341 cggttgaaga ggaacggttt ctgcgcgaca agcacgcaaa gaaccgcatg ccgttggaat
    26401 cggcccgcta ctgtctccgc gccggtggga tttcgccaag ggacgtggac gtggtcgcgt
    26461 ttccctacgc gccgatcgcg ctgtccagcg ctgcgcgctg gcactacgcc agacgccact
    26521 ggtacgcgcc cgatcgggcg ctcgatgcgc ttttcaacgg caaccgtcgg tatcgccgaa
    26581 accggcggcg cgttttagag ctcggcgacc agctcggcat cgactggagc cggacccgat
    26641 tcgagcccgt ggagcatcat cttgcccacg cctccagcgc ctaccacctg agcggcttta
    26701 cggagaagac ggccatcatg ggggtggatg gcaagggtga gtacgccacc acgttcttcg
    26761 gctacggcga gaacggccgt attcaccgga tcaaggaatt ctacgacccg gactccctga
    26821 gcggcgtcta cggcgcgatg accgaatacc tcggcttcga gatgctcgac ggtgagtaca
    26881 aggtgatggg catggcgccc tacggagatc ccgacctata cgatctcggg cgcctgatcc
    26941 gccgagcacg cgacggcttc gaggtggacg tgaactacgt gaacacggtg ggcctgcgac
    27001 gtcacaagga gtcgggcgtt ggcttctact tcagtgacaa gctcatcaac tggctcggac
    27061 cacgccggca aggggaggtg gctgacgagc cctacatcca ctacgccgca tccatgcaga
    27121 agctcttcga ggagtggtgc ctggaactca tggagagata cctcggggac gtgctgcggg
    27181 agaccgggcg gctggctttt gcaggcggcg gagccctcaa cgtgaagctg aaccagcgaa
    27241 tcgttgccct ggatcacgtt cgtgagctgt tcgtccagcc ggcggccgga gactcgggta
    27301 cttcgctggg ggccgccacc tacgtggcag ccagcgaagg tcaacgccta tcccccatga
    27361 cccacgtctt tctcggtccg cgttacgaca acgacgattg ccttcaggcc cttcgagccc
    27421 gtcgcgagtc ccttcactgg cggcgcctgg acgacgtgcc cgctcgggcg gcggaacttc
    27481 ttgcccaggg gcatccgctg gcgtggttcc agggccgcat ggagttcggc ccccgggccc
    27541 tgggcgcccg cagcattctg ggttgtccca gcacgcccgg gattgccgat cgcatcaacg
    27601 agcagatcaa gttccgcgag aggtggcggc ctttctgccc cagcgtgctc gatcgggtcg
    27661 cagcggaaat catccagacc gagcatcccg cgcctttcat gaccattacc ttcgatgtgg
    27721 cgcaggaatg gaaatcgcgg atcccggaag tcgttcacga ggacgggacc gcccgtgtcc
    27781 agatcgtgga tccggacagc aatccccggt actacgcgtt gctcgagacc ctggaaacgc
    27841 tcacgggcaa cgcggtggtg ctcaacacgt ctctcaaccg gcggggagaa cccatggtct
    27901 gctcgccgga agacgcgctg aacatgttct gcgggtccga tcttcagttt ctcgtgctcg
    27961 aggattttct cgtcagcaag acggacgagg aagctcagta gagctttccc tcgcccgggg
    28021 gacgcgtctt gaaccggcga tgaatccaga ggtactgatc cgggtgcgcg cggatcgcgt
    28081 tctcgatcaa ttggttgacc cgtgccgcgt cggccacctc gtcgctggtc gggaacccat
    28141 cgagcggctc gctgaacgtg acgtgccagg tcatcgtgtt ccagtcgcga aaatggctgg
    28201 caaagatcac gggcgagccg gagatccgcg cgaggcgaga cgtggcggtg atggaggcgg
    28261 cgggcgcgcc gaagaaaggc gcaaagacgg agtgcttggg cccgtagtcc tggtccggcg
    28321 cgtaccagat cgtgcgcccc gccttgagac gcgcgatcgt ctggcggacg tcggagcgct
    28381 cgatgacgcc gtcgcagtag cggctccgcc ctcgaacctg cagccactcc cagacgggat
    28441 tcttgttggc gcggtatatg acatcgaggt ctacggcgcg ggaaaccgca ttggccatca
    28501 cgtccaggga cgtgaagtgg ccgcccagaa gcagcacgcc cttcccttcg gcctgcgcgg
    28561 cccgcagatg ctcgaggcct tcgatggtca ctcggtcgcc gaggtcgcgg tcagggtcca
    28621 gccagaccac catggtctcc agggcgccca ccgcaacgtg ctgaaacacg ccccggatca
    28681 tctcccgtct cgcggcttct cccagatcgg gaaaacacag cctgaggttg acctcggcga
    28741 tgtgtcgtcg tgaccgggcg aggcggtagg cgatacggcc cagccatcgc ccgagccccc
    28801 cgatcgcaga tatgggcaga cgcgcgagca gccagccgat ggcaacgatg agccaggtcc
    28861 cccagacgcg aggagagtac attcggggtc ggctctttct gcgcctgctc atgaggttcc
    28921 accggcgggg taggcgaatt gtaacgggga ggccctgtga taccatccac gccccatgaa
    28981 tctgccgtcg ctgcaagggg ttcacgtctt ggtggtcggc gatctcatgc tggaccgcta
    29041 ctggattggt ccggccgagc gggtgtctca ggaagccccg gtcccggtgg tcagggtgga
    29101 gcgggtagag gagcatcccg gaggcgcggc caacgtggcc ctcaacgtcg ccagtctcgg
    29161 cgcgcggtgc accctcattg gttacgtagg taaagacgac gcggccgaag ccctcgtcga
    29221 caggctcacg gcagccgggg tcgcttgcga cctggtcgtg gtagacggtt ggtccaccgt
    29281 ggtcaagctg cgggtggtca gccagaacca gcagctcatc cgcaccgatt tcgaaagcgc
    29341 tctgatcccg ggcgaggacc atcgcgcgct gctgcgtgaa aaggtcattg cccggctgtt
    29401 ggatgcttcg gccatggtgc ttcaggatta cgacaagggc gtgatcgagg accccgaaac
    29461 gctgatcggc tccgccatcg cggcgggcgt gccggtggtg gtggacccta aacgcaagcc
    29521 ctttgcccgc tatgcgggag ccgccatcct caagcccaat gcaaaggagc tcgaggcggc
    29581 gctcggctcg tgggacgacg acacggatct cgtctcattg tcggcggcgg cctgcagaac
    29641 ccatcgcctg ggtgccatgg tcatcacccg gggtgccggc gggatgacgg tcgttgcggc
    29701 cgacgggcag caccggcaca tcccggcctt gccggcggac gtttacgacg tgacgggcgc
    29761 gggggatacc gctgctgccg cgctcgccgt gaccaccagc ctgggctggg atgcggaccg
    29821 cggtgcccag gtggcgaacg tggcgagcgg gctggtggtg gccaaggcgg gaacggcggc
    29881 gatcacgggg cccgagcttg cgcttgcgct ggccgggcgt catcgggtgg accgcggtgc
    29941 tctgcgcagg gcgcagctgg aggacgcggt cgctcaggcg cgagcggccg gcgagcggat
    30001 cgtcttcacc aacggctgct tcgacatcct tcacgcgggc cacgtcgcgt atctggaaga
    30061 agcccgggcg ctgggcgatc gcctggtggt ggccgtcaac gacgatgact cggtggcgcg
    30121 tctgaaaggc ccaggtcggc cggtgaattc tctcgaacga cgcttgcgtg tgctggccgg
    30181 gctcgcgtcg gtcgactggg tcgtcgggtt ctcggaggat acgcccgaag ccctgctgga
    30241 gctgttgaaa ccggacgtcc tggtcaaggg gggcgactac gggcccagcg aggtggttgg
    30301 cgcggatcag gtcatcgccc agggcggcga ggtgcgcgtg ctcggtctgg tggaggattg
    30361 ctcaaccact gccatcgtcg atcggatccg gggcgcttag gcgccgagcc cgggatgtgc
    30421 cggcgcgggg ccggcttcaa gccgggcaga cggcggccac gttggcggcc aggtgctccg
    30481 gatctatggt tccggtgacg atgcaggaca cgccgggctg tgccgccacc cactccagtg
    30541 attcgggccc cgcgaagccg cttgccaggg ctttctttac gagcacgccg cagccgatgc
    30601 gtccggcctc cgccacgatg ggggcctgct cgcggtcggc cgggttcagc accgtcatga
    30661 tcacgtcgca gccggcggcc agggcaagcc ggccgccgtc gggggttttg tgggagatcc
    30721 cgatggctcg cacgcggccc gaagcctgca gctcccggag cgcatccagc gtgcccaggt
    30781 tctccaggat ctccacatcc gctccgctgg agtggatgag gacgatgtcc aggcggtccg
    30841 tgcgcaggcg ctcgagagac ctctcgacgc tgcgccgtgt gtgctcgggc gagaagtcga
    30901 aacgggacac gccgttcgag aactcctcgc cgaccttcgt gcagatgatc cagtggtccc
    30961 gctcggcacc gatcagcgcg ccaagccgtt cctcgctcgt gccgtaggcg ggggcggtgt
    31021 cgaggaggtt cacgcccagt tccctggctt tcttgagcag ggcggcggcg gcccgggcgt
    31081 cgggaatggt gaacgggcgc ggatacctga cgccctggtc gcggcccagc ttgaccgtgc
    31141 cgagaccgat gagcggcacc tcgatgcccg tgttgccgag gggacggtac tgcatgctag
    31201 gtttcccagg gtgagcggcc gacccgggcc gtgggcaggc ccaggtccgg cgagccgggg
    31261 tggggagccg atggcggatc cagcagcccg agcaccttgt cgccgagatc gggtaccagg
    31321 ctcagcttgg tgggccagca caccacgcag cttccgacgg ccgctgcgaa cgcctcgtcg
    31381 ggcctacccc ctcgggtctg ctcgggctct gcccggtcga tgcgcagggt ctcgaatcgc
    31441 gcatcgtccc acgaaagcca ggggacacag gcttcgagct cgcgccgggc ccggtcgatc
    31501 agggcggggg catctagctt cacgccgtcc gttgccagct gcccgccgag ataccacagc
    31561 cagtgatcgc cgtcggggtg gctggtgatg gtcaggcgcg gctccgcgcg gcggatcccg
    31621 gtcaggcagt gagcgtacat gggatgcgga tgctcatgtc ggaccaccac ctgatgcagc
    31681 gggcgcagct gcatgcgcgg tctccgcagc cccaggccgt cgagcagcgg ctgggagccg
    31741 gcgccagcgc agatgatgag gtgtctcgcc gtcaacgacg cgtccgggag ccggagcacc
    31801 acccgatccg cctcgacgag cagatcctcc ggggcgacgg cgtgctgaaa gacgcatccc
    31861 cgcagcgtgt cgttcagctg cgagacgagg gcgccggtgt cgagcacgaa gtcgcgcagc
    31921 gcgtagacga cgccatggaa ggcggggttt gcgaaaacgc aggggtaatc gtgcggctcg
    31981 accttctcga tccgtccgcg cagggagcgg ctggcaaaga accccgtcaa cctgcccagg
    32041 ctcgacgact cggcaaacag ataataccgc tcggccaagg gagcaagggc ggaaagatcc
    32101 aggtcgcccg tcccgtccag gcagcttcgc cagcgctcgg gcatatcggc aatggcctgc
    32161 gaggcccggg tgagccggcc ggtgagcgca tacttcagcc cgccgtgaat catgccctgg
    32221 ctcgccagcg tctggccggc gcccagccgg tccttttcga gtactaccac ctgatatccc
    32281 tgccgcacca ggaggttggc cagccacagg ccggcgatgc cgcccccgac gatgatcgcg
    32341 tccgtctgcc agtctcgccc gctcatggga gcgcgagcct acggtttcgt ccgcagggcc
    32401 gcaatcccgg gcattcttga cggggaccgc gcgcctgcgc aggcttgcgc catgtggcgg
    32461 atcctttatc aggtgctcct ccccctggcc tatccgctgg tccggttgcg gctgctgtgg
    32521 cgcgcgcgcc gtgagcccga gtacgggcga cgtatccccg agcggttggg gcgggtgccg
    32581 gcatcagtgc ccaggggcgt catctggttc cacaccgtct cggccggcga gaccatcgct
    32641 gctgcgccca tcatcgatcg tctgttaagc gagtttcccg agagcgactt tctggtcacc
    32701 accatgacgc caaccgggtc ccagcaggtc atcgcccgcc taggcggacg ggtcgctcac
    32761 tgctacgcgc cctatgactt ccgcggcggc gtggagcgct tcctcgacgc cgtcgccccc
    32821 cggctgctgg tgatcttcga aaccgagctg tggcccaacc tcctggaggc agctacccgg
    32881 cggggtattc ccgttgatct ggtcaacgcc aggctgtctg aacgatccgc gcgcggctat
    32941 gcccgaatcg gggccctgac gcgcagaatg ctcgggcagc tgcagcacat cgcctgccag
    33001 tacccggccc acgcccagcg attcgtcgcc cttggcgccc agcccgggcg ggtgtccgtg
    33061 ctcggcagcg tcaagtttga cctgcgcctc cccgacgacc accgcgcccg atgcgccgag
    33121 ctcgcccgtc gatggcggct ggaaggccgt ccggtctgga tcgcggggag cactcatccc
    33181 ggtgaggatg cgccggtcct ggcggcgcac gatgcggttc gcagcgcttg tcctggtgcg
    33241 tgcctgctgc tggtgccgcg gcacccgagc cgggcggcgg acctggagaa gatgattcag
    33301 gaggcgggat tttccgtggt caggcagagc gtcgctgccg gcgcggaagc aggggtgacg
    33361 gtggacgtgg tgatcggcga cgtcatgggg cagctgctct acctgtacgg tttgtcccag
    33421 gccgctttca tcggcggcag cctggtgcct gtggggggac acaaccccat tgagccggcc
    33481 ctgtgcggtc agcccctgct gacggggccc cacacgttca atttccctga cgtggtggcc
    33541 gcgttcgagg actcggcagc tctcttgcgg gtcggcgacg cggccggcct tgccgctgag
    33601 gtcgcgcgct gcctgggcga tgccgagttg cgggagagga tggccgcgtc cgcgcgccgg
    33661 gtcgtcgccg cgaataccgg agcgaccgag cggttgctcg cgcttctgcg cgagcacgtc
    33721 ggccgcgtta gcggaggcgg tccgctcagt tgatgactcg ggctttcagc gagttgagcc
    33781 gctccacggg gtcattggag tccgcgtaca cgttcagctg ctcgacgtcc tccccgttga
    33841 gggtgccggc cgtttgcctg agctgcatga gatcgatgat gtagttgtag cgggagtcgg
    33901 cgtagtcgaa ctcggatgcg tagagtcggt tctgcgcatc cagcacgtcg acgatgttgc
    33961 gggtgcctac ctcgtagccg gtctgggtgg cttcgagggc cgacttgctg gactcgatgg
    34021 ccttcttgcg cgcccggacc cggaccacgt cggtggcgac cgcgcggaac aggttgcggg
    34081 tatcccggct cacggtcagc tgccggtcca gcagctcctg acgcgcctgc tcggccagag
    34141 cccgagcctc ccgcgcacgc gacgaggtca ggccgccctg gtagatgggc agggtgatgg
    34201 acagcgcgta gacgtcctga tcgattttgc ccccgagaaa ggaagcaccg ccggtgacga
    34261 aatggttggt gctcaccgtg gcatccaccg tgggcatgtg ccccgagcgc cgcgccttga
    34321 gggttcgctc cgcggcgtcg agctgggagt tggccgcgag gatggtcggg ttgccggtca
    34381 atgcggtgtt cacccactcc tcctcgttgc ccggcgtcgg gtcgaccacg ggcagcgctt
    34441 ccgagatgcg cgcgatgctg tcgtaaggct gtccggtgag ggtgcgcagg gtttcgaaga
    34501 agatgtcgtg atcgcccgcg gcctggatcc ggaccacctg ggcgctgtcg tagacggcct
    34561 gtgcttccag cacgtctgtg atggccacca ggcccacgtc gaagcgctgt tgaacctgct
    34621 ccagctggcg cttcaccgcg tcctctgccg ctttcgcgga gtccagcaga tcctgggccc
    34681 gcaggacgtt gaggtaggcg accacggtgc gcacgatcag atcctgctcc accgacacga
    34741 agccccattc ggcgccctcg acgccggcct gagagctgcg gaagttgaac caggcttcca
    34801 ggtcgaaaag gggctggcgc agctcggcct gccagccgtg ctcgttgaat tcctgatcgg
    34861 gaatccgtac ccgggtgcca atgtcctcta taaagacggg tgacgggaag ctccgctcgt
    34921 tccaggatgt ctgcccgccg atggcgactc tgggcaggag cgccgaacgg ctctggggta
    34981 tcagttcctg ggttgcatcc aggccggcac gggcagcgcc cagaacgggg tcgttgctct
    35041 gtgcgtcccg gtacgcgtcc aggatatcgg tagcgctcgc ggcagaggcc cacgccaggc
    35101 ccagcagggc gatgatcaat ctgggtttgt tcaaggcaat gtccgtcttt gctgttagga
    35161 agagagcgcg catattagca aacgcaccct gcaaagcacc gcggagctga tcgtcggacg
    35221 gttgggcggg gcgttgaact ggttcccatc cctgagccat ctgcccctgg aattgtgggc
    35281 cgttgcctta aattagccgt cgcagcgctg tcaacggtga cgcctccgcg cggcgtcggt
    35341 atattggcag gtgaaatggc cggcttttcg tggacagtgc ccgacaaacg ttacagcatt
    35401 gatttgcccg cacacatggc tgaatgcgat gccaactatc tgcggctgat gaagctcttc
    35461 ccctccctgc gcgaggaaga cgtgagcgac tttgccgtct gcatcggcga catgcactac
    35521 gaggtttgca tcgaggtaca ggatcgaggc ccgtacacca cgctcgtgcg gctcagccag
    35581 ctacccgaag cgccctggag cacgcggccg gcgctcaccg ttcgtgtcta tcacgatgcc
    35641 cgcagcgcgg aggtggtgga gtatcagcgc ggcggccact ttcaggcggt ctacgattat
    35701 ccgaacaggg acatgcgcca gcccgacgag aaggcgcagg tgaatcgatt cctgggtgag
    35761 ttcctcgcca tgtgcctgac tcacggggtt tccgtgcgcg aaccggagtt cgccagctga
    35821 gcggctccgg aagtcgcacc cccccggagc ccgacgtcgg ccgcgcggcc atcgaaacct
    35881 gaaaaccgtt ctccacatta ccgacctgca tctgcagccc cgggaaagcg accggttgct
    35941 gggcgtggac accgcggaca gccttcgtgc cgtcctggca agagccctgg cggagggccg
    36001 cccggacgct ctgctggtca cgggcgacgt cagccacgaa ggggatgagg ccagctaccg
    36061 gcgcttcgcg gcgatcgtcg ccgatcacta cgagggcccg gtgctctgcc tgcccggtaa
    36121 ccatgacaaa agcgctgcga tgcgggaggt gctgggcgag acgtgggcgc tcgatctgca
    36181 ggagtggtcg ctcatcgggc tggacagtca tgtggacgga caggtgggcg cggggctttc
    36241 agagacggac atggcggcgc tcgagggccg gcttgcggca tcgcgagggc gccacgtgct
    36301 cgtcgccacg caccatcctc cggtggacgt gggatgtccc tggctcgata aagaccgcat
    36361 ccaaaacggc gccgagctgc tagaatggct gtccgagcac gccggtgtaa aaggcatggt
    36421 gttcggccac gcacatcagg tcatcgagtc tgcctacgaa aacctcgctc tgctgggcac
    36481 gccggccaca tgctttcagt ttgctccgcg cacggagagg ttcgccgtgg acgagcgcaa
    36541 gccgggatac cgctggctgc gcctggatgc acagggaggc ctgcagacgg aggtgggtcg
    36601 ggtcgacgat tacccgctca cactcgacct gtccgagcgc agccgaagcg ctggccgggc
    36661 ccccaaaccc aactgaagag gtccaatccg ttcatggctg acgccagcaa gtattccgct
    36721 gaatcgctgc aggtgctctc cgggctcgag cccgtgcgcc gccgcccggg gatgtacacc
    36781 gatacctccc ggccgaacca tctggtccag gaagtggtgg acaacagcgt ggacgaggcg
    36841 ctcgccggat atgcgcggga aatcgaggtc acgctgttca aggatggctc cgtggaggtg
    36901 gtcgacgatg gccgcggcat gcccgtggac gtccatccga agcacaaggt ttccggggtg
    36961 gagctgatcc tcacccgcct tcacgccggc ggcaaattcg acaacgagaa ctacagcttc
    37021 tccggcggcc tgcatggcgt gggcgtttcc gtggtgaacg ctctctccga atggctggag
    37081 gtggaagtaa agcgcgaagg caaggtcttt cgccagcgct acaagggcgg caagcccacc
    37141 agcaagctga aacccgaggg gaccgtgggt cagcgcaaca cggggacccg ggtcacgttc
    37201 aagcccgatg ccggatactt cgactccgtc aatgtcgccg ttggcaggct caagcatctg
    37261 ctgcgggcca aggccgtgct ctgtccgggt ctgcgcgtga gcctaaacgt ggaaggcaag
    37321 gaatccgagt cctggcacta cgagaacggt ctggaaggtt acctggttaa cgcgctcgcc
    37381 ggtgccgagg taattcccga gaagccgttc atgcacgccg ccactggcaa ccaggaaggt
    37441 gtggactggg ccgtgcagtg ggtgctcgat ggcggcgagc tgattaccga gtcctacgtc
    37501 aacctgatcc ccacggctca gggagggacg cacgtcaacg gtctgcgttc gggcctcatc
    37561 gatgcgctca aggaattctg cgagttccgg gatcttcttc ctcggggggt caaactcacc
    37621 ggcgacgatc tctgggatca gtgcagctac gtgctctccg ccaagctcaa agacccgcag
    37681 ttttccgggc agaccaagga aaagctctct tcccgccagt cggccgtttt cattggcggc
    37741 gtggccaagg acgccttcag tctctggctc aacgagcacc cgcaggacgg cgagcggctg
    37801 gcggagctgg ccatctccaa cgcgcagaag cgggtccagg cggcaaagaa ggtcgcgcgc
    37861 aagaaggtca ctgcgggtcc cgctctgccc gggaagctgg cggactgctc cggtcaggac
    37921 gccgagcggg ccgagctctt tcttgtcgaa ggagattcgg ccggcggctc cgccaagcag
    37981 gcgcgggatc gagagttcca ggcggtcatg ccgctgcgcg gcaagatact caatacctgg
    38041 gaagtggatg tcagtcaggt cctcgactcc caggaggtgc acgacatcgc cctggccatc
    38101 ggggtggatc cgggctcgcc ggacctgagc ggcctgcgct acgcgaaggt ctgcatcctc
    38161 gcggacgcgg actcggacgg cctgcacatt gctacgcttc tctgcgcgct gtttctgcgc
    38221 cacttccgcg cgctggtcga cgccggacac gtgttcgtcg ccatgccgcc gctttaccgg
    38281 atcgacgtgg gcaaggaagt cttctacgcc ctggacgagg cggagaaaga gggcgtgctg
    38341 gaccggatcg cggcggagaa gaaacgcggg cagattcagg tgcagcgatt caaggggctt
    38401 ggcgagatga acccgatgca gctgcgcgag acgacgatga atcccgacac ccgtcgattg
    38461 gtccggctcg aactcgactc accgacgaag accgacgagg tcatggacat gcttctggcc
    38521 aagaagcgtt cgcccgatcg ccgcgcctgg ctggagaaga agggcgacca ggcgagcctc
    38581 gcgtagcctt cgaacaaccg caatttggag gcgccagatg gcgccatgga aagcatggaa
    38641 acggaaaatc ccggcataga aaccataccc ctgcgggcct atacggagcg ggcgtatctc
    38701 gactacgcca tgtatgtcat caacgatcgc gcgttgcccc acattgccga cgggctcaag
    38761 cccgtccagc gccggatcat ctttgccatg agcgagctgg gcctttcgaa tcaggccaag
    38821 ttcaccaaat cggcccggac ggtcggcgac gtgctgggca aattccatcc ccacggcgac
    38881 agcgcgtgtt acgaagccat ggtgctgatg gcccagccgt tttccttccg ctacccgctc
    38941 gttgacggcc agggcaactg gggcgcgccc gacgacccca agtctttcgc ggccatgcgc
    39001 tacacggaag cgcgcctgtc tgcctacgcc gagctcatgc tttcggaatc caagcagggc
    39061 acggtggact tcattgccaa cttcgacggc acgctcaccg agccccggct tctgccggcc
    39121 cgggcgccct tcgtgctgct caacggcagc acgggcatcg cggtgggcat ggccaccgac
    39181 atcccgccgc acaacatgaa cgaggtcatc agcgcctgca ttcacctgct cgactcgccg
    39241 aaggccaccg ttgccgacct catgacgcac atcaaggctc cggacttccc gtccgaagcc
    39301 atcatcatca ccccgccggc agagatccgc gaggcctacg agaccgggcg cggatcggtg
    39361 aaggcgcgcg cggtgtggaa aaaggaggcc ggggacatcg tcgtcacggc gctgccccat
    39421 caggcgtcgc cggctaaggt gctagagcag atcgcccagc agatgaacgc gaagaagctg
    39481 ccgatgctgg cggatctgcg tgacgagtcg gaccacgaga acccgacccg gctggtcctg
    39541 gtgcctcgtt ccaatcgggt ggatgccgag cgtctgatga gccacctgtt tgccaccacg
    39601 gatctggaga aaagtttccg gatcaacctg aatatcatcg gtctggacca gcgccctcag
    39661 gtgaaatcct tgcaggccat tctcaaggag tggttgacct atcgcctgga aaccgtgcgc
    39721 cggcggctca cgtttcgtct cgaccggatc aacgagcgac tgcacatcct cgacggcctg
    39781 ctcaaggcct atctgaacct cgacgaggtg atccggatca tccgggaaga cgatcacccg
    39841 aaggcttccc tgatgaagcg gttcacgctg tcggaaatcc aggccaacgc catcctggac
    39901 cttcgcctgc ggcaacttgc caagctggag gaggacaagc tcaaggccga gaagtcggaa
    39961 ctcgagtcgg aaaaagcgga catcgagaag acgctcaaat cgaaagcccg catgaaggcc
    40021 ctgttgaagt ccgaactggt cgagatcgcc gagggttacg gcgacgctcg ccgatcgcct
    40081 ctgggtgcgg tggaagatgc ggcggcctac agcgaggaag atctgatcag caacgagccg
    40141 atcacggtgg tgctctcgga gaagggatgg gtccggtccg ccaaaggcca cgaaacaaac
    40201 cccgaggagc tgagctaccg gagcggcgac ggctacgggc acgcggcccg gggccgaacc
    40261 aacgacattc tggtctttgt ggacagcacg ggccgctgct ataccgtggc ggctcacagc
    40321 ctcccctctg cccggggaca gggcgagccg ctcaccgggc ggctcaaacc tcccgcggga
    40381 tcgaggttcg tcggcgtcgc cctcggaccg ggcgagtcga aggtgctgct ggcaagcgac
    40441 gctggctacg gtttcgtcgg ccggctgtcg gggctcgtcg ccaagaacaa gtcaggtaaa
    40501 gcttgtctca gcgtgcccaa ggggggcctc gccctgcccc ccctgctgct cgccgatcag
    40561 ccggacctgc aggtggtggc cgtcaccgac cagggtcgga tgctcgtctt cccgctgacg
    40621 gaggtgcccg aactgacccg cggcaaggga aacaagcttc tcaacattcc gtccgcggct
    40681 ttcaaatccg gcgaagagcg gctggttgcg gtgacggtca ttcgtcccga cgacgagctg
    40741 ctcgtgctgg cggggcagcg tcatctccgc atgaagcaca gggatctcga gaactacgtt
    40801 ggtgagaggg cacggcgcgg gcgcaagctt ccgcgcggat ttcagaaagt agactcgctg
    40861 gcagttgcgg gacggtgacc ggcgaggtgg gcggcctggt gctgcaccag atcctgctgc
    40921 ggggctgaca gcgctgtaat caggaccgag tcgggaccca ccgttgccag ctcctcgagc
    40981 agggggtgct cgatgaccgt gcgctccacc agctcgccgc cgcggtcgag gcaggcggag
    41041 aaaacgctgc tcgcggccag ggtggccggc ttggcgagcg cggacaggag cgcggcatcg
    41101 ccgaccgcct gctcgtccaa cagcacgcga tggccgaccg gcgcgtcgcc gacgtgaatg
    41161 gccactcgat agtcacccgc ctcctcgtcg agaagctccg agagcaccgc cgctgcgctc
    41221 agcacctgcc agggacggtc cgcatcgccc ccgtcgttga agtccagcag caatccggta
    41281 ccgggcagat tttccacatc gccgccgtac aggccggcga ccttgcccgc ggcgatgcgc
    41341 gcgtagttca gctcggcgac gcgttgcgtg ggctccatgc cgagctggtc gtacaggttc
    41401 acggcgacga ggaagtgcct ttcccgcggc gtgtgaagct ccacctctat acgcgcgggc
    41461 tgcctcggca ggggcggttg aagccggatg ctgagcaggg ccagcgccag gaagggcgca
    41521 gcaatcaagg tgacagcggc caccgccacg gcatccccga ggtggcttgg aggctcgctc
    41581 agggcgaccc gcacggcgcc catgatctgg tcgttgaagg tcaccgggct ctggtagacg
    41641 cgcgcgttcg aaagggagcc gctcgaggcg agcgcctgct cgttgaccgt tacgatttcg
    41701 accccgctga cgccgggaat gtccgccgcc tgattggcga tcacgcccag cgtgatgcga
    41761 tcgtgggtca gcagcggctc gatggccagc tgggcgagcc ctgtggacat gctcctgcca
    41821 taggcgtcgt gacggagctg atccggatcg ttgagagccc agtaggacag catcacggcc
    41881 acggaaacca gcgagcccag gcaagcgccg accagcgaaa gcggcggtgg tttgacgagc
    41941 cccagccaag acgttagctt caaactactt cctattccgc gcggcgatgt gagcagcgag
    42001 attgtattgg tgaacgtgtc cggagaggac cagcctggcc tgatggccat gttgacatcc
    42061 actctggcac gttatcaagt gcgggtgctc gacgtggggc aggcggtgat ccacgacgag
    42121 ctcaacctcg gcatgttgct gcagatcatc agtgacggta acggcgtgga ggccgccgag
    42181 gtgctgtccg ccatcgaggc gaacgttctc gatcagggca tgacggcgcg cttcactccc
    42241 atctcggcgg gcgactacgc cgactgggta aagagccagg gcaagccccg ctatatcgtc
    42301 accctcctca cggccggcgc caccggcgag cagctcgccg cggtgacctc catcgttcgt
    42361 caccacggcc tcaacatcga cgccatacga cggctgtcgg ggcgcttgcc cttgacgtcc
    42421 gacgccggtg gtcagcggat ctgcatcgag atgagcctgc gcggcgtcct tgccgatgcc
    42481 ggcgccctga aagcgggcct tctcgaagcc gccgagcgcc tggcgttcga tttcagcgtg
    42541 cagcaggaca ccgtgtaccg gcgcaaccgg cggctggtgg cgttcgacat ggactctacg
    42601 ctcatcgatg ccgaggtcat cgacgagctg gcggccgcgc acggggttgg cgagcaggta
    42661 gctcggatca ccgagcgagc catgcgcggt gagctgaagt tcgccgacag ctttcgagag
    42721 cgggccatgc tgctcaaggg tctttccgag gacgccctgc gtgaggtggc agacggcgtt
    42781 gcgctgaacg acggtgcccg gcgcctgatc cgcacgctca agcatttcgg ctacaagacg
    42841 gccatcatct caggcggctt tcagtacgtt ggtgagcggc tgcgtgacga gctgggcatc
    42901 gattacgttt atgccaactc gctggagatt cgcgacgggc tgatgtcggg cgaggtcgaa
    42961 ggggagatca tagacgccgg ccgcaaggcc gagctcttga aggagatcgc ccgccgtgag
    43021 gggatatccg gccagcagac catcgccatc ggcgacggcg cgaatgacct gcccatgctc
    43081 aatgccgccg gtcttggggt ggcataccac gcgaaaccca tggtcaagga gacggctagc
    43141 cacgccatct ccaccttcgg cctggacagc gttctgtacc tgatgggctt cagcgaccgc
    43201 gacatcgacg aagcgcttac gggctgaagc cgccgccccg gcgatatccg gtcagcgggg
    43261 atcagtcgtc ggcgctgaag tcgtaatcca tacttctgga gggctcctgc aggtagtgcc
    43321 cggccacgta gttggcaccg gcctgccata gggtcgacag gatgttggcg ctttccacca
    43381 tggggaccac cgtgagcttg ccggcgccct ggaggccgtg aatcatctcg gtcagcgcct
    43441 cacgcgcctc cgagctgctg gcgatcttct cgatcagcga accgtcgacc ttgacgtatt
    43501 ccgccgggat ttgttcgagc agttcgaaag agttgccgag gcgcccaaat cgcgacagcg
    43561 aggctacgca gtgcatctgc ttcaactgct cgaagaactc gcgggtctgg cgaacgtagg
    43621 tgttggcgtc ctgctccgtg atctgcagga ccaccgcatc ggtaggcagc cgaacggcct
    43681 tgaaggcgac gccaagccac tccaggaact ccgggtcgtt gacgctgttg ctggtcaggt
    43741 tgatggtcag ccgggtcagg ttgcccttgg cacggtgcgc agacagcgac ttgatcgcct
    43801 ggagaatgac ccatcggtcg atccgtccgg cgaggttgtt ctcgatggcc gtgcgcagga
    43861 aagcgcccgg gagcatctgt ttgccttcgt cgtcaagcat gcgcagcagc acttcgtagt
    43921 gctcgtcgga gtcgccgtgc aggctgatca tcggctggaa cagcagaacg aatcgctggt
    43981 cgtcgatggc ctcgttgatc aggcgcagca gccttgctga ttcggactcc ggctcgtcgc
    44041 ctgctccctg ggacttcagc tcgacgcagt ttccttcgtt ctgttccatg gatgtgcgca
    44101 ggacatcgta ggcctcgttc agacagtcct ccaaccgctc acggacgccc tgatcttcct
    44161 ccgtggcgat ctccatgccg gcgagggtaa tcgtgggccg tacggtcttc tccctgacct
    44221 cgagcagcga tgcctcgatg gtcccgcgaa gctgatcggc ctctgctcgg acctcgtcct
    44281 cgttgtcggc ggttaccgca aaggcgaact gatgatccgc cagtcgatgc agcgcgtccg
    44341 atgcgatcag ctcgccgaga caccgctcga catgacggca gacctcgagc gctccagcca
    44401 gaccgtgaga tcgctgcacc tcggcgtaat cgtcgatctc ggcgacgaaa atcgcggaaa
    44461 atgcgtcctc ggagcgggtc ctggggttgc cggccaccgc gtcgaagaag gcgtgaagcc
    44521 tcacatcggc tggcgcgctg ccttcgccgt tggcagtcga agcatcgtcg gctgcggcct
    44581 gcgcggtcgc aggggcgtcg gttgcccggc cggttcgcac ggtgacctgc atgcagtgct
    44641 cgccctcgta ctcggcggcg gcgagggtca tggtgcccgc aatggtctct tcggccgtgt
    44701 tctgaccgac gaactcgatc tctttttcct cgccgtcgcg ctggaactgc ttcatcgcga
    44761 ccttcaaggc cacggcgctg tcggcgtcga gcagatccat gtgcggcagc ccgatgaggt
    44821 cctccacgtc ttcaaagccg aagagcttca ggtaaccctc gttggcgtag atgtgcatgc
    44881 cgtcgtggac gtaggcgatg gcggctttgg agcttctgag cagcagctga cagcgctgct
    44941 cggtttcctg gagtgcccgg cgtgtctggc tcaggctctg gtactggaga acgtgctcga
    45001 gctcccgctt gaccaccagg acgaggtgct ccggctcgga tttcgggacc acgtcggcgg
    45061 cgccgagctg catgccgcgc gtggtcgtga tcgtcgcgtc atcgtaattg acgagaacga
    45121 tcggaacgtt gggcgcctgg tcgctgagcc gcgggatgat ctgctctagc tcgggcagag
    45181 aagagttcgc cagcagcaga tccgcttcgt gcatggcgtc gacggccatg ggaagatcga
    45241 tgatctccga ccgggtggga atgcctgcgt cgcgcaggag cgagtcgaac tcctgcgccg
    45301 cattctcgga attctccgca attagcaggc gcacgggcgc gctgggattg tgctgagccg
    45361 tcatatcctt tttagccaac ctttcccttt tttcgaatca cccggacgtc gggcattgga
    45421 cgtccgcgga ggaactcatg gtgcggccga aacgtggcca atccaagtaa ccatccaaca
    45481 gacgaaatgt gaaccgggtc gcgctttgac cagagagcgt gaatccgagc aagtccggaa
    45541 gccccggcgc cgctccgaag ccggctatcc cctcgacggc caagcggata tgatgccgcc
    45601 gattccacac tggtccgctg cacatggcga ctgtttctac caacgagttc cgctcgggac
    45661 tgaaggtcct tctggagggc gagccctgct cgatcctcga gaacgagttc gtgaagccgg
    45721 ggaagggcca ggccttcaat cgggtcaagt ttcgcaatct gatcaccggt cgggtttggg
    45781 agcggacgtt caagtcgggc gacagcctgg aagccgcgga cgtcatggag ctcgacatgg
    45841 agtacctgta ctcggacggc gacttctggc acttcatgcg caccgacggc agctacgagc
    45901 agtcggcggc gccgccgagc gcggtgggtg acgccaagga ctggatcaag gagcaggagg
    45961 tctgccaggt gacgctctgg aacgacgagc cgatctccgt cacgccgccg aacttcgtcg
    46021 agcttcaggt gaccgaaacc gacccggggc tcaagggtga taccgcccag ggcggcacca
    46081 agccggccac gctgtctacc ggcgcgacca tcaaggtgcc cttgttcatc aacgttggag
    46141 acgtgctgcg cgtcgatacc cgcaaaggcg agtacgtcag ccgcgcttga acgcgccacc
    46201 ccccgcctgg cgcccggcgt gctcgattga agcgctcggc gcgcgcgccg ccatgctgcg
    46261 gacgatccgg gatttctttt tcgagcgcgg ggtcattgag gtgcagtctg cctgcctcgg
    46321 cgcgcacacc gtcacggacc cggccgtcga ctcgctggcg gtgcccggga tcggctatct
    46381 gcagacatct cccgagtatc agctcaagcg cctgctcgca gccggggcac cttcccttta
    46441 ccagctcggg cccgtatttc gcggcgggga ggcgggacgg ttgcacaacc ccgaattcac
    46501 aatgctggag tggtaccggc ttggctatac cgacgcggag ctcatggccg aggttgccga
    46561 gctgatcgac tcggtgctgg gggccgccga ctacgagagg ctcagctacg aacgggtcgt
    46621 tgccctgggc ggtggcgccg agagtggggc ggagcgtgca gagctggacc tcgcgtttgc
    46681 ggatgggatc gccgccctcg ccggccgccg ggtgttcgtg gtggactatc cggcagatca
    46741 ggcaatcctc gcgcggctgc gccccgggcg gccgaatgtt gccgcgcgtt tcgagctgtg
    46801 cgtcgacggc gtggagcttg caaacggcta ttgggagctc ggcgatcgtg ccgccatcga
    46861 gcgccgcttc gccgaggatc tcgccgcgcg caggcaagcg ggttccgagc tgcctgagcc
    46921 ggacggcgcg ttcctggacg ctatggacgc ggggctgccc gaatgcgccg gcgtggcgct
    46981 gggcgttgat cggttgctga tgatcaagct gggtgcgggg agcctggcag aggtcatggc
    47041 ctttcccgcc gaccgggcct gatcagtcgc agaaagcgcc tatcgcttcg cccatgcgaa
    47101 cggtcgcgcc ggccttgatg tgtggcagca gctcgatcct gccaggctcg aagcagacga
    47161 tcaccgtgga gcccagcaca aagcggccga tctccgcgcc ccgtgcgaag gcgtcatcgt
    47221 aggaggtcgt cgtgacgctc tggtagggcg actcggggcc cgcccacacc gtctcgatgc
    47281 ttgcaacgat gagcgcgccc accagaacga cgagcatctg gccgtggccc gtgcggaatc
    47341 ggcagaccag ccgctcgttg cgcgcgaaca ggccctcgac ggtggtctcc gtctgcgcgt
    47401 tgacggaaaa gagcgccccc ggaatcgccg tggtggcgag cagggtcccg tccgccggca
    47461 ggtggacgcg gtgatagtcg cttggtgcaa gatagacggt tgcgaacgtt ccgccttcga
    47521 atgcccgatc agcctcgctc gtcagcgagc gaagcgagta ccggtgccct ttcgcctgca
    47581 gcagctgacc gtcccggatc aggcccgtct ggctcacggt cccgtcagcc gggcagagca
    47641 cggcggccgg atcccgggcc agcggccggg cgtcttgacg tagcgcgcgg gtgaagaagt
    47701 cgttgaagca gcggaaatcg tccagctcct gacataccgc ctcgtcgagc gacaccccgt
    47761 aggctcgtgc gaacccgtga atgaagggtt tgcggatcca gggcgcttct gatttcgcca
    47821 gggcgccgac gaaccgggac agctcgtgct ggggaagcag cctctgcagc gcggcgaacc
    47881 aggcgctcac gaccggctca ccggggtgtc gtcgcgattg ccccactcgg cccaggatcc
    47941 gtggtaggcg cagattcggg ggtagccgag cagccgcgcg accatgtagg agaggccgga
    48001 gcggtgatga gtctggcagt gggtgatgat gcgcttgccc ggcacgattc ccctggattc
    48061 gagcagggag cgaacgtcgt ccaccaggcg caggtcgcgc tcggggtcct tgaggtgcat
    48121 ccagtcgacg ttcaccgcgc cgggaacgtg gccggcccga cgggaaccgc tgcgcagccc
    48181 cagatactcc tcgcgagagc ggacgtccca gaccacctga tcgtcgtcct cgatggcagc
    48241 catgacgtcc tcgagctccg cgatgggttc gccttgaatg tcgatcgtga cgtttgctgg
    48301 cggcacgggc agccccggtc cggtctcaac cggcagcccg gcggccagcc agctgtggat
    48361 gccgccgttc aagtaggtcc agcgggtgtg gcccaccaca tccagcgtcc acgccatccg
    48421 gccggcccag ccgccgccct cgtcgtcgta gacgatgacc tcctggtccg gatcgaaacc
    48481 gattcgcgag aaaagcgctt ccagcctctc cctgctcggc agccggccgg tagccggcgg
    48541 cgcgccgcca atcagctccc caggctcgac gagaacggcg ccgggaacgt gcccctgcag
    48601 gtagccggcc gcatcgccca catgcaccac gagccggccc tccgggtcgg cgccggccag
    48661 ctcctcgggt tcgatgattc tcggtggcgc gatgacggct tcctgtccgg ggcaaaaggg
    48721 ccgcctatct tagccgagaa ctgaatcgcc gtggcagatc ccaggcttgt ggcagaatgg
    48781 ccggcggcgg gcgggcgacc cgcatccgtt taggagtggc aatgccgaca aacctggtgt
    48841 ggatggatct cgagatgacg ggtctcgacc ctgaaaccga cgtcatcatc gaaatggcca
    48901 cggtgatcac cgacagcgat ctcaacattc tcgccgaggg cccggtgctc gccgtttggc
    48961 aaccccaggc aaagctggac gccatggacg agtggaacca gacccaccac ggcgagtcgg
    49021 ggctcattga tcgcgtgcgg gcctcagcgg tctccgagcg ggaggcagag gcgcggaccc
    49081 ttgccttcat cgagggccac gtcgagcgaa agcaggcgcc cctgtgcggg aacaccatct
    49141 ggcaggacag gcggtttctc acccgctaca tgcccaccct tgagggctac ctgcattacc
    49201 gcaacatcga cgtgagcagc ctcaaggagc tggtggcacg ctggcggccg gagcttctga
    49261 aagggatcaa caaagccaat cggcacaccg cgctgtccga cattcgggaa tccatcgagg
    49321 agctgcgtta ctaccggaag cacttcatcg cgttgtcctg acggctcacg gctcagatcc
    49381 gcccgatcgt cgggtcaatc ttttgatcgc ccagtcgatg tgttcgcaaa ccatggccga
    49441 tgcgcccgcc cgcgcttccc gcagggcccc cagagcggcc gcgctgggag agccgttgcc
    49501 cagcgccacg gcgaggtttc ggcgccactg atcccagctg atgcgccgaa tggccgaacc
    49561 gcgcgtgttc ctctcgaagg tctccgggga ccagccgaac aggtccagca gcgttgccgc
    49621 ttcgagccca tggcgtggct ggaagtccgg ctcgttggtg acgccggcaa agcggttcca
    49681 cgggcagtag atctggcagt cgtcgcagcc gaagatgcgg tttcctatgg gcgcgcgaag
    49741 cggctcggga atcgcctcct tgagctctat ggtgagatag gagatgcacc ggcgtgcatc
    49801 cagctgcccg ggcgcgacga tggcatccgt cgggcacacg gtcatgcagg cgttgcagtt
    49861 gccgcagccg gcgccgtcct cgcccggctc gccgggttca tcgagcggca acggcaggtt
    49921 cgtgtagatc tcgccgagaa aaaaccacga tccgcctttc gagttgagga gcagggtgtt
    49981 cttgccgatc cagcccagcc cggccttttc cgccagtgcc ttctcgagca cgggggctga
    50041 gtcggtgaag gcccggtacc gcccttgcat acccgcggcc gcagagtcga ttctccgggc
    50101 aagggttgcc aaccggccgc ggatcagctt gtggtagtcg cggcccagcg cgtagcgcga
    50161 gacgtaggct cggtcgggat tctccaaagc cgccctgggg ctggtagggt cggtcaggta
    50221 atccatcctc gcgacgatga cccgacaggt ttcggtctcg agcttttccg ggtgaaagcg
    50281 cttgtcgaga ttccgctcga ggtacgacat ggtgccctgg aagccccttt ccagccaggc
    50341 tcgaacgcgg ggctcgtggg ccgacagatc gacgtcggtg acgccgatct gctgaaagcc
    50401 gagctcgccg gcccacgtct cgatgtcctt gcgcagcgct gctaggtcgg ccatccgctc
    50461 tgaatccacg tatcggcggg gaagccaccc agtataatgc ctggtcttca tcgacggtga
    50521 tgtggaggat cgagccctga atcgcgttta caccggccgc gagtgccgcg agctcgaccg
    50581 catcgccatc gaggagttcg ggattcccgg cttcgagctc atgatgcgtg ccgggcgcgc
    50641 cgccttctcc gagctgctgg cccgctggcc cgatgcctcg tgcctcacga tcctgtgcgg
    50701 caagggcaac aacgcggggg acgggtatgt catcggcggg ctcgcccggg aaatcggcat
    50761 ggccgtgcag ctggtccagc tgggcgatgc cgcggacctc gcgggcgacg ccgcgcgggc
    50821 gagagactgg gccgtcgagc gtggcgtcct catcgagccg ggaccggaaa gagcgctgcg
    50881 tggcgatgta ctggtcgacg ctctgctggg cacgggcatc aagggagagc tgaagccggc
    50941 ctatcgcgag caggtcgagg ccgtcaacgg ctcggggatc agcgtgctcg cggtggacgt
    51001 acctaccggc gtggatgcgg acaccggcgc ggcatgtgag ccttcggtca gggccgacgt
    51061 cacggtgacc ttcatcgctc gcaagctggg gctggtcacc gggccgggtg catcgttctg
    51121 cggcaacctg gtgcttgccg gcctcggcgt gcctgatcag gtgtacgact cggtagccgg
    51181 cactgccctc ctgctgcacg aaaacctgga agcgctgccc gctcgggatg ccaatgccta
    51241 caagcaggcg ctcggccacc tcgtggtcgt tggcggcgat cacaccatgg gcggcgccgc
    51301 gctgatggcc ggggaagccg cgctgcgggt tggcgccggg ctggtgagcg tcgtgacccg
    51361 cccagcccac cggccggcca tactcgcccg gcgcccggag ctgatggtcg tggatgccga
    51421 cgacgaccag agcaggcgcg aggtcctcgc ccgggcaacg accatcgtgc tgggacccgg
    51481 cctcggccga agcgcgtggg gcgagcggct gtcgcgggaa gcgctggccc tcggcaaacc
    51541 ggccgtgatc gatgctgatg gtctttactg gctcgcgaca ggcgcgctgg agccgacggc
    51601 gcccgtcatc atcaccccgc attcgggaga agcggcgatg ctgctcgaca gcacctctgc
    51661 cgcgatccag gcagaccggg tgacggcggt cgagcatctg gcggagaagg ttgccggcgt
    51721 cgccgtgctc aagggcgccg gaacgcttat tgccgaacat ctcggctcga cgagccgcct
    51781 ggcaggcgtt tgcggtcacg gcaatcccgg gatggccagc gcaggcatgg gcgacgtgct
    51841 gagcgggatc atcggcgggc tgctcgccca gcacgatgtg ctcctcagtg ctgccgtcag
    51901 gggcgtatgt ctgcacagcg ccgccgccga tgtggtggca agacaagtcg gtcaacgcag
    51961 catgctagca acggatcttc tgccggaagt ggtgtccatg ctggcggccg ccgagcagtg
    52021 agaccggtcg cggtggaatc cgtgtttctg ccggacgcgg aagccacgga ggcgttcggg
    52081 cgcgacctgg cggtccggct cgagcccggc gatctggtgc tgctgcatgg ggacctcggt
    52141 gctggaaaga caaccctggt caggggcata ctcgcgggtt taggtcattc cggttcggtc
    52201 aagagtccca cgtatactct gctcgagcct tatgaattga cgtcgtgtgt cgtcctgcat
    52261 ttcgattttt atcgaatagg ggacagccgg gaactcgagt tcatcggatt tgacgagctg
    52321 atggacgcag atgccatcaa gctcgtggag tggccggagc gagcggccga tcgcctgcct
    52381 ccgcctgatc tcgagataac cctgcgcgtg gaagacgaag gtcgtcgtat tgaagtcgaa
    52441 gctgctcgtt agcgcatttc tgctcctttg ttacgcgccg ccatcctcgg ctggcaagct
    52501 ggaccgagtg cgcctgcacg aggcccccga tcacacccgc gtggttttcg acgtggccgc
    52561 acctgtcgag ttctcttatt tcacgttgcg caacccggac cgggtggtca tcgatctcaa
    52621 ggacaccacc gcgggaaacg acttcgatcc ttcgcggacc gggactaaat cgagccgtgt
    52681 gaagaacatt cgcgcagcgc cgaggggacc cgactatcgg gtggtgctgg acgtttccgc
    52741 ccccctcgag ccgaaggctt tcgcgctcga accggttgcc gcgtacggcc accgtctggt
    52801 ggtcgatctt ttcggcgccg caccgaaacc cgccgcgccg gctgcgccga aggccgatga
    52861 ccggcgtgac gtgatcgtcg ccatcgatgc cggtcacggg ggggaggatc cgggcgcgct
    52921 cgggccaggg aaggtgcgtg agaaggacgt ggtcctgagg atcgcgcgca agctcgaagc
    52981 gaggctcaac gcaaccaacg gctacaaggc cttcatggtg cgggacggcg actactacat
    53041 cgcccttcgc aaacgggtgg acctggcacg ccggtcccgg gccgacctgt tcgtgtcgat
    53101 tcacgcggat tcgttccgca gccccgaagt ctcgggcgct tccgtctaca ccctctcctc
    53161 ccagggggcg accagcgaga ccgcgcgctg gatggcggaa aaggagaacc ggtccgacct
    53221 catcggcggc gtggggggcg tcagcctgga tcacgatgac gatctgctga accacgtgct
    53281 gctcgatctg tccaccagcg ccaagcaggc ggccagccac gaggcggcca agtcggttct
    53341 cggccagatg gcccgcgtca ccaagctcca caagagccgg gtggagcagg cgggcttcgc
    53401 ggtgctcaaa tcccccgacg tgccatcgct gctcgtggag acgggctaca tctcgaaccc
    53461 ggcggaggcc aaccgcctgg ccagcgcccg gtttcaggac aagatcgcca gcgcgttgtt
    53521 cgagggcgtt cgaagctaca tgacgcgctc cgctccgccg ggaacgctgc ttgcctggca
    53581 gcgggaaagc gggccggttc gttacaccat agagcggggc gataccctct ccgacattgc
    53641 ggtcaggtac ggcaccagca cgcggcgcct gagggaagcc aacgggatcg cgggagaccg
    53701 gatccgggtg gggcaggtga tcgtcattcc agcatcgtga gccttctgcg agaatgacgg
    53761 gccatggccg gtcgaattca agtactcgag cccttcgttg ccaaccagat cgcggcaggc
    53821 gaggtggtcg agcgcccggc ttctttagtc aaggaactcg tcgagaacag ccttgacgcc
    53881 ggcgccaggt cggttcggct ggagaccgag ctgggcggca tgaagcgcgt cctcgtcagc
    53941 gacgacggcg agggcatcga tgcggccgac ctgccgctgg ccgtcgccag acacgccacc
    54001 agcaagatct cgggacccga cgacctggtc ggcatcggca gcctcggttt ccgcggggag
    54061 gcgctggcca gcgtggcgtc ggtggccagg ctgacgatca cctcggcaac ggccgaggcc
    54121 gctcacggaa cccgcctgga agtggatggc ccgggagacg ttcacatcgg tcccgccgcc
    54181 cacccaaggg ggacgacggt ggaggtgcgc gatctctttt tcaacactcc ggcccgacgc
    54241 aagttcctga agaccgagcg caccgagcag tcacaagtag atcaggtgct ccggcgcatc
    54301 agcctgtgcc ggttcgacgt cgccttcgag ctgcgccatg gcggcacccg gtcggttctg
    54361 tcgctgccgg caggagaccc cgagctgcga cttgcccggg taattggcga ggccttcgtg
    54421 aagcgctcgg tggccctgga cgagacgcgc ggggagctca ggttgtgggg gtgggtcggg
    54481 ttgcccacct attcccgcag ccaggcggat cagcagtatt tctatgtgaa cgggcgcgtg
    54541 gtgcgagaca agctcgtcgc ccacgccgtt cgccaggctt accgggacgt ccttttcggt
    54601 ggccggcagc cggtcttttc cctttacctg gagctgccgg cggacgccgt tgacgtgaac
    54661 gtgcacccga cgaagcacga ggtccggttt cgcgaggcgc ggtcggttca cgatttcatc
    54721 ttcggtactc tggccagagc gcttcgcgag gtgcggccgg atgccgtggc cgcgcctgtt
    54781 gcgggcgaag cggcggcgac cggttccccg gggtcgtgga ctcagggtcg ccaggccggg
    54841 ctgggtctgc ctcacgcggt ccaggatcgg ccttttccgc cggccccgcg gagcctgcag
    54901 gaagcgttcg ccgagcacgg cgcgccgatc acggtcacag aatccgacca gccgattccg
    54961 ccgctcggct atgccgtcgc tcagctgcac ggtgtctaca tcctggctca gaacgcggac
    55021 gggctggtcg ttgtggacgt gcacgcggct cacgagcgcg tgacctacga aaagctcaag
    55081 cgacagatgg atacggaagg cgtgccgcag caacgtctgc tggttcccgt gccggtgagc
    55141 gtcagcgcgc gcgatgcgga tctgctcgag cagttcgagg acgtcgtctc ggccactggt
    55201 ctttccttag acagatccgg gacgcagagc ctgatggtgc gcgcggtccc cgcgcttctg
    55261 gccggcgcca atgctgagga gctcgttcgc gacgtcctgg cggacctggc cgagctcggt
    55321 tcaagcgagc aggtccggaa ccggcagcat gatctgctgg ctaccatggc ctgccacggg
    55381 tccgtgcgtg cgaaccgtga gctcactctg gctgagatga acgcgctgct gcgggagatg
    55441 gaagtcacgg acaacgccgg gcagtgcaat cacggcaggc ccacgtacat ggttcagtcc
    55501 atggcggagc tggaccggct attccttcgc gggcagtaga cccatcgatc ccatgacagt
    55561 accggcatcg ggttccgtgg cgctcgtgat caccgggcca acggcggtgg gcaagaccga
    55621 gcttgccatc gagatcgccc ggcgagtgcc ggccagcctg atcagcatgg attccgccct
    55681 cgtttaccgt ggcatggacg tcggcacggc caagccttca ccggagagct tgatcgagca
    55741 cccccaggct ctgatcgacg tccgtgaccc gaaagagcca tactcggctg cggagttcgt
    55801 cgccgatgcc gacagcctcg ttcaggaggc ctgggcggcg ggtcgcgtgc ccgtcgtggt
    55861 gggcggcacc atgatgtatc tgcgagcatt tcgggaaggt ctcgccgatc tgccccaggc
    55921 cgatccgcag ctgcgcaggg ccatcgctgc cgaggcggag tcccggggat gggaggctct
    55981 tcacgagcag ctgcgcgccg tggatccggc cgcggcgcag gtcattcatc cgaacaaccc
    56041 ccaacgcctg cagcgggcgc tggaggttca ccgcctcacc ggccggccca tatccgactt
    56101 ctggtcggag cagcgcaacg ccgattttgc ggcgcgcctt ggcgggagga tggagaccat
    56161 tgccctgatg cccgaccgtc gtgaccggct tcacgctcgg atcgcccgtc gatttgcagg
    56221 gatgctggag cgggggctgg tggaagaggt gcggcaattg cgcgctcgcg gcgatcttca
    56281 cgcggacctt ccgtccatgc gggcagtggg ttatcgccag gtctggcagc atctcgaggg
    56341 cgagctcgac gcagccgaga tgcgcgagcg tgcgtgcgcg gcgacgcggc agctcgccaa
    56401 gcgtcagctc acgtggctgc gggggtggtc gtgggtcagg gaggtcgagt ggggcgaccc
    56461 ggttgcgctg gcggagcaag ccctgctgcg cgtggaccag ccccacggaa gtgctcgacg
    56521 cgcttgaaat atgggcaggc tgtagccata gtgaatgtgg cggtaaaagg acgagcgctg
    56581 cgtccccgaa aagtcctgca aagccgtcga ttggggggcg aacttgaagg agctgcaaat
    56641 gtcaaaaggg cattcgttac aagaccctta tctcaacacc ctgcgcaagg agcgcattcc
    56701 cgtatcagtt tatctcgtca acggtatcaa gctgcagggc cagatcgagt cgttcgatca
    56761 gttcgtggtc ctgctgcgga actctgtgag ccagatggtc tacaagcacg ccatctcgac
    56821 cgttgtgcct tcgcgcaacg ttcgcctgtc gggccccggc ggctccgaag aggctgcgga
    56881 ggaatagacc ggttcaggcc ggctcttccc ttggcggaag gcgggcctga aaggagcaca
    56941 cttgtttttt gaacgacccg aagcaggccg tagatcggtc ctgttgcagg ttcacctgaa
    57001 gagcgctgcg gcgcccgatg cagccgtcct tgccgaatcg gagctcgaag ccatcgagct
    57061 tgcccgttcc gctggagtcg acgtctgtca ggtggtctcg gcgcggcggg ataaccctca
    57121 cccgaagtgg ttcgtcggcc ccggcaaagt cgccgagctc gccgaggtgc ttgcctcgca
    57181 ggaagccgat ctccttatcg tcaacaacga gctgaacgcc ggccagcagc gcgatctcga
    57241 gcgcgatctg ctctgccggg tgctcacgcg taccgagctt atcctgcaca tctttgcgga
    57301 tcgtgcccga acctacgagg gtcagctgca ggtggagctg gcgcagctca gacatgccca
    57361 gacccgcctc gtccggggtt ggacgcatct ggatcgtcag aagggtggca tcggcctgcg
    57421 gggtgcgggc gaaacccaga tcgagatgga ccagcgggcg ctgggggagc acatcaagcg
    57481 ggttcagcgc aagctggagc aggtggcacg gcgtcgcgat cagaaccggc gccgtcggcg
    57541 ccgcacccgc acccgcacgg tctcgctggt gggatacacg aacgccggca agtcgaccct
    57601 gttcaacgcg ctcaccagag ccggggtgct cgtcgaggac aagctgttcg ccactctgga
    57661 tccaaccatg cggcgactga cggttcccgg cgcgggcgac gtcgtgctga gcgacaccgt
    57721 cggcttcatc aatcagctcc ctcacgacct ggtcgatgcc ttcaaggcga cgctcgaaga
    57781 ggtcagcaat
//
LOCUS       DBNHLM010000080         5800 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1029012, whole genome shotgun sequence.
ACCESSION   DBNHLM010000080 DBNHLM010000000
VERSION     DBNHLM010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1029012"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="AC16QF_03915"
     CDS             complement(<1..1273)
                     /locus_tag="AC16QF_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HIR6447728.1"
                     /translation="MLTTLRRIVHGVEESSSFKEALSLLVGEVREALGTEVCSAYLLN
                     PEGDSYVLVANEGLNTDAVDKLSLGLNQGLVTLVAERAEPINLSDATHHPRFYLVPEI
                     GEEAFNAFLGVPVIANRRVLGVLVVQERDARRFDESEEAFLVTLAAQLATVIAHHEAT
                     GEIQQLLEDSSVEPIAREDALYKGVPGAPGISLGTAVVMHPTASLEAVPERIAEDVRT
                     ELMVLDRAVNAVRDDIRRISEQFAQSLPPEELALFDAYLHMLDDNALAGDVRDRINRG
                     QWAQGALKQVIREHVRRFEAMEDPYLRERGADVRDLGMRVLAYLQDIRSRKTYFPDDT
                     ILVGHEITPSMLANIPPEKLKGIVSVRGSSNSHVAILARALGIPAVMGAMDLPIFSME
                     NISVVVDGFYGEVFTNPSLALAGQYLQLIAEE"
     gene            complement(1267..1764)
                     /locus_tag="AC16QF_03920"
     CDS             complement(1267..1764)
                     /locus_tag="AC16QF_03920"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HIR6447729.1"
                     /translation="MVDADGFRPNVGIVLSNSIGQVLWARRVGGQDAWQFPQGGIREN
                     ESVQAALFRELFEEVGLVADDVEVLASTRGWLRYRLPRRMRRYNSSPGFQGQKQKWFL
                     LRMLGDDARVQMDGSAKPEFDSWRWVSYWYPLDQVVSFKRDVYRAALKELAPHLPVED
                     VASPC"
     gene            1906..3981
                     /locus_tag="AC16QF_03925"
     CDS             1906..3981
                     /locus_tag="AC16QF_03925"
                     /inference="COORDINATES: protein motif:HMM:NF015168.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR6447730.1"
                     /translation="MSNRLADETSPYLRQHADNPVDWHPWDEAALALASEQQKPILLS
                     IGYSACHWCHVMAHESFEDPATARVMNEHFVNIKVDREERPDLDKVYQLAHQLLTQQG
                     GGWPLTMFLDPDTRLPFFGGTYFPKTPRYQLPGFVDLLLRIAETFASQRDELRDHGTR
                     VADVLGRLAGGDSQAGELGDAELLAAAREQLGAQADEDEGGFGGAPKFPMPETVTYLL
                     RHWAYAQRSGEADRQGLEFVMTTLTRMARGGIYDHLGGGFCRYATDRRWMIPHFEKML
                     YDNGQLLALYSDALAIGPDELFSQAVSDTADWLLREMRHPQGGFYAALDADSEGEEGK
                     FYLWRRDQVKRLLDEDEYLVVETLYGLDKPANFEGKWNLHRYDAWRSVVERLSMDRAA
                     ANDLLASARRKMLAARELRVRPGLDDKVLTAWNGLAIKGLARAGSRLGQSRWLDAAAQ
                     AADFARARLWRDGMLYATWQGAPRYPAYLDDYANLLDGLIALLGARWREEDAAFACKL
                     ADGALERFYDADRGGFFFTAHQHETLIHRPKPTMDDALPPGNGVMVQALTALGHLFGE
                     TRYLEAAAATLAWARAAMEQYPAGHCCLLSGLQDEIMPPELVVVRGAAATFPDWRRAA
                     VSGYTPWRAVYCIPFEGVSTLPAYLPGPVSDDPHSQTVAYVCSGLSCSLPIGKPEDLG
                     EALRSRPRV"
     gene            complement(3978..5441)
                     /gene="gcvPB"
                     /locus_tag="AC16QF_03930"
     CDS             complement(3978..5441)
                     /gene="gcvPB"
                     /locus_tag="AC16QF_03930"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HIR6447731.1"
                     /translation="MSDVRTIFELSAQNRGAAAQHLSERPGAMDDIPASLRRERAPGL
                     PEVSELQVVRHYTNLSRKNFSIDTNFYPLGSCTMKYNPRGAHRAASLPGFLNRHPLAP
                     DSASQGFMACLHELQEILKDVTGMQGVSLTPMAGAQGEFAGVAMIRAYHEARGDHARN
                     EILVPSAAHGTNPATAAMCGYRVAEVPVNASGDVDLEALKLAVGPQTAGLMMTNPSTC
                     GVFERGIESIAAVVHDAGGLLYYDGANLNAILGKVKPGSMGFDVLHMNLHKTFATPHG
                     GGGPGAGPVGVGERLLPYLPVPFVAQDAKGAYRWVTDAELPDSIGRLSAYAGNAGILL
                     RALAYALMLGREGMHRVGEYSTLNANYLARRLEDAGFELAYPDRRATHEFILTFSESA
                     RSRDVTAMDFAKRLLDYGIHSPTTYFPLLIPECFLIEPTETETKAELDAFVDALVAIQ
                     REAEADPEKVKGAPYDLPVRRLDDVKAARELDLAWRG"
     gene            complement(5438..>5800)
                     /locus_tag="AC16QF_03935"
     CDS             complement(5438..>5800)
                     /locus_tag="AC16QF_03935"
                     /inference="COORDINATES: protein motif:HMM:NF014408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447732.1"
                     /translation="NQGLLVTAATIHMSLLGPVGLARIAEHCHANTRALVEALTAIDG
                     VEARHQGPFFHERVLQLPRPADEVVAGLLERGILGGVALGAYHESMNHDLLVCATEKR
                     TDDEIGRYAAALREVIAR"
BASE COUNT          984 a         1935 c         1888 g          993 t
ORIGIN      
        1 gctcttccgc gatgagctgg agatactggc cggccagggc cagcgaaggg ttggtgaaaa
       61 cctcgccgta gaaaccgtct acgacgacgg aaatgttctc catgctgaag atcggcagat
      121 ccatggcccc catgaccgcc gggatgccca gggcccgcgc cagaatcgcc acgtgggagt
      181 tgctcgagcc tcgcacggat acgatgccct tgagcttttc cggtgggatg ttggccagca
      241 tgctgggggt gatttcgtgg ccgaccagga tggtgtcgtc ggggaaatag gtcttccgcg
      301 agcggatgtc ctgaagatag gcgagcaccc gcatacccag atccctgaca tcggctccgc
      361 gctcgcgcag ataagggtcc tccatggctt cgaagcgccg gacgtgctcg cgaatcacct
      421 gcttgagggc gccttgtgcc cactgtccac gattgatgcg atcgcgcacg tcaccggcga
      481 gcgcgttgtc gtcgagcatg tggaggtagg catcgaagag cgcaagctcc tcgggaggca
      541 ggctctgagc gaactgctcg cttatcctcc ggatgtcgtc gcgcaccgcg ttgacggctc
      601 ggtcgagcac catgagctcg gtgcgaacat cctcggcaat gcgttcgggc accgcttcca
      661 ggctcgccgt cgggtgcatg acgaccgcgg tgcctagact gattcccgga gcaccaggga
      721 cgcccttgta aagagcgtcc tcccgggcga tgggctcgac cgagctgtcc tccaggagct
      781 gctgaatctc gccggtggcc tcgtggtgag cgatcaccgt cgccagctgg gcggcgagcg
      841 tgaccagaaa ggcctcctcg ctctcgtcga accggcgcgc atcgcgctcc tggaccacca
      901 gcaccccgag cacccggcgg ttggcaatga cgggcacgcc gaggaacgcg ttgaacgcct
      961 cctcgccgat ctccggcacc aggtaaaagc ggggatggtg ggtggcgtcg ctgaggttga
     1021 tgggttccgc ccgttccgcg accagcgtca ccaatccctg gttcaggccg agggaaagct
     1081 tgtcgaccgc gtcggtattg agcccctcgt tggccaccag cacgtagctg tcgccttcgg
     1141 ggttgagcag gtaagcggag cagacctcgg tgccgagcgc ctctcgaact tcgcccacca
     1201 gcaggctcag cgcctccttg aacgagctgg attcctcgac tccatggacg atccgccgca
     1261 gcgtggtcag catgggctgg ccacatcctc aacgggcaga tgcggcgcaa gctccttgag
     1321 cgcggcgcgg tagacgtcac gcttgaagct gacgacctga tccaacgggt accagtagct
     1381 cacccaacgc caggaatcga actcgggctt ggcgctcccg tccatctgca cgcgcgcgtc
     1441 gtcgcccagc attcgaagca gaaaccactt ctgcttctgc ccctggaaac cggggctgga
     1501 gttgtaacga cgcatcctgc gcggcagtcg gtagcggagc cagccgcggg tgcttgccag
     1561 cacctcgacg tcgtccgcca cgaggcccac ttcctcgaac agctcgcgaa aaagcgcggc
     1621 ttgtactgac tcgttctcac ggatgccgcc ttgcggaaac tgccaggcgt cctggccgcc
     1681 aacccggcgg gcccaaagca cctggccaat gctgttcgac agaacgattc cgacgtttgg
     1741 gcggaaacca tccgcatcaa ccatcggcat tcctctgaat ttccctcgat tgaacctcaa
     1801 tatgggccga attaccaact gcggccccga cgggccgacc gagttgaccc ggtcgtgttc
     1861 cgtgtcagga tgctgaccct gcacagccga aaagagcctc gaaccttgag caaccgcctg
     1921 gccgacgaga caagccccta cctgcggcaa cacgccgata acccggtaga ttggcacccc
     1981 tgggatgaag ccgcgctcgc gttggccagc gagcagcaga aacccatcct gctctccatc
     2041 ggctactcgg cctgtcactg gtgccacgtg atggcccacg aatccttcga ggacccggcc
     2101 actgcccggg tcatgaacga gcacttcgtc aacatcaagg tggaccggga ggagaggccc
     2161 gatctggaca aggtctacca gctcgcccat cagctcctca cccaacaggg tggcggctgg
     2221 ccgctgacca tgtttctgga ccccgacacg aggctgccat tcttcggcgg cacctatttc
     2281 ccgaaaacgc cccgctatca gctgcccgga ttcgtcgatc ttctgctgag gatcgccgag
     2341 acgtttgcca gccagcgcga cgagctgaga gatcacggca cgcgggtcgc cgacgtgctc
     2401 ggccggctgg caggcggaga ttcacaggcc ggcgagctcg gtgacgccga gctgctggcg
     2461 gctgccagag agcagctcgg cgcgcaggcc gacgaggatg agggcgggtt cggcggggca
     2521 cccaaatttc ccatgccgga gaccgtgacc tacctgctgc gccactgggc ctacgcccag
     2581 aggtcgggcg aggcggaccg gcaggggcta gagttcgtca tgaccaccct gacgcgcatg
     2641 gcgcgaggcg ggatatacga ccacctgggc ggcggcttct gccgctacgc cacggaccgt
     2701 cgctggatga ttcctcactt cgagaagatg ctttacgaca atggccaact cctggccctc
     2761 tacagcgacg cgcttgccat tggtcccgac gagctcttct cccaggcggt gagcgatacc
     2821 gccgactggc tcctgcgcga gatgcgtcac ccccagggcg gcttctacgc cgcgctggac
     2881 gcggactccg agggcgagga ggggaagttc tacctgtggc ggcgcgacca ggtgaaacgg
     2941 ctgctcgacg aggacgagta tctggtcgtc gaaacgctct acggcctgga caaaccggcc
     3001 aacttcgagg gcaagtggaa tctgcaccga tacgatgcct ggcgctcggt ggtcgagcga
     3061 ctgtccatgg atcgcgccgc tgcgaacgac ctgctggcca gcgcgcgccg caagatgctg
     3121 gcagcccggg agctgcgggt tcgccccggc ctcgacgaca aggtgctcac cgcctggaac
     3181 ggcctggcca tcaagggcct ggccagggcc gggtcacgcc tggggcagtc gcgatggctc
     3241 gatgcggccg ctcaggcggc cgatttcgcg cgcgcccggc tctggcggga cggcatgctc
     3301 tacgcgacct ggcagggggc gccccgctat ccggcctacc tggacgacta cgccaacctg
     3361 ctcgacggcc tgattgccct tcttggggcg cggtggcgcg aagaggacgc cgccttcgcc
     3421 tgcaagctgg ctgacggtgc cctggagcgg ttctacgatg ccgacagggg cggctttttc
     3481 tttaccgccc accagcacga gaccctgatt catcggccaa agcccaccat ggacgacgcg
     3541 ctgccgccgg gcaacggcgt gatggttcag gcgctcacgg cgctgggcca ccttttcgga
     3601 gagacgcgct acctcgaggc tgcggcggcc actctggcgt gggcgcgcgc ggccatggag
     3661 cagtatccgg ccggccactg ctgtctgctc tcgggcctgc aagacgagat catgcccccg
     3721 gagctcgtcg tcgtccgcgg tgccgccgcg acgtttcccg actggcgccg ggccgcggtg
     3781 tcgggataca cgccatggcg ggccgtctac tgcataccct ttgagggcgt cagcacgctg
     3841 cccgcctacc ttcccggacc cgtgagcgac gatccgcaca gccagacggt ggcatacgtg
     3901 tgcagcgggc tgtcctgctc gctgcccatc ggcaagccgg aggatctcgg cgaagcgttg
     3961 agatcccggc cccgtgtcta gccgcgccag gccaggtcca gttcgcgagc ggccttgacg
     4021 tcatccaacc gcctgacggg cagatcgtaa ggcgcgcctt tgactttttc gggatcggct
     4081 tcggcttcgc gctgaatcgc caccagggca tccacgaacg cgtccagctc cgccttggtc
     4141 tccgtctcgg tgggttcgat cagaaagcac tcgggtatca gcagcgggaa ataggtggtc
     4201 ggagagtgaa tgccgtaatc gaggagtcgc ttggcgaaat ccatggccgt cacgtcccgg
     4261 ctgcgtgccg actccgaaaa ggtcaagatg aactcatggg tcgcccgccg gtccggatag
     4321 gccagctcga agcctgcatc ttcaagacgt cgcgccaggt agttggcatt gagcgtggag
     4381 tactcgccca cccggtgcat gccttcccgc ccgagcatca gcgcgtaagc gagggcgcgc
     4441 agcagaattc cagcattgcc ggcgtaggcc gacagacggc cgatgctgtc gggcagctcg
     4501 gcgtcggtta cccaccggta tgcgcctttt gcatcctggg cgacgaacgg caccggcagg
     4561 tagggcagca gccgttcgcc gacgcccacc gggcccgcgc ccggcccgcc gccgccgtgg
     4621 ggggtcgcga acgtcttgtg caggttcatg tgaagcacgt cgaagcccat ggaccccggc
     4681 ttcaccttgc cgagaatggc gttcaggttg gctccgtcgt agtagagcag gccgccggca
     4741 tcgtgcacca ccgccgcgat gctctcgatg ccacgctcaa agaccccgca ggtggacggg
     4801 ttggtcatca tcagccccgc cgtctggggt cctaccgcaa gcttgagggc ttcgaggtcg
     4861 acgtcgcccg aggcgttgac gggcacttcc gctacccggt agccgcacat cgccgccgtc
     4921 gccgggttgg tgccgtgggc ggcgctgggc accaggattt cgtttcttgc atgatcgccc
     4981 cgggcctcgt gataggcgcg gatcatggcc acgccggcga attcgccttg tgccccggcc
     5041 atgggggtga gggacactcc ctgcatgccc gtgacgtcct tgaggatctc ttgaagctcg
     5101 tgcaggcagg ccatgaaccc ctgactggcc gagtccgggg caagcgggtg ccggttcagg
     5161 aaacccggca ggctggcagc gcgatgcgcg ccccgcgggt tgtacttcat ggtgcatgag
     5221 ccgagcggat agaaattggt atcgatggag aaattcttgc gcgacaggtt ggtgtagtgc
     5281 cgcaccacct gaagctccga tacttcgggc aggccgggag cccgctcacg acgcaggctc
     5341 gcgggaatgt cgtccatggc tcccggccgc tcgctcaagt gctgagcggc ggcaccccgg
     5401 ttctgcgcgc tgagctcgaa tatcgttcgc acatcgctca tcgggcgatc acctccctca
     5461 gggcggccgc atagcggccg atctcgtcgt ccgtccgctt ttctgtggcg cagacgagca
     5521 ggtcgtgatt catgctctcg tgatacgcgc cgagcgccac gccgccgagt atcccgcgct
     5581 cgagcaggcc ggctacgacc tcgtcggccg gcctgggcag ctgcaacacc cgctcgtgaa
     5641 agaacggccc ctgatgccgc gcctccaccc cgtcgatggc ggtcagggcc tccaccagag
     5701 cgcgggtatt ggcgtggcaa tgctcggcaa tcctggcgag cccgaccggc cccaagaggc
     5761 tcatgtgaat ggtggccgcc gtgaccagca agccctggtt
//
LOCUS       DBNHLM010000081         9529 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1148468, whole genome shotgun sequence.
ACCESSION   DBNHLM010000081 DBNHLM010000000
VERSION     DBNHLM010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9529)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9529
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1148468"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="AC16QF_03940"
     CDS             <1..1334
                     /locus_tag="AC16QF_03940"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR6447733.1"
                     /translation="QLVRSAFAPDGGLADDVVIAGGAQESVQQPQWLGGDALIFLSDR
                     NGYSNLYRYDPSGLYCTLEDGADYGVPQWVFGIRQFAAVSDRHVVAARQGTETELVMV
                     DTEGGFATPLTDAGGAASLGSVVKWQDSVCCLAEFPDRLAAIVRCPLDGSPPTAIHQP
                     GTAPLGAELISRAERVQYPTRDGNRAFAYYYPPRNPERPVTGAEGELPPLLVMSHGGP
                     TAAASRALNLRIQYYTTRGWAVVDVDYRGSSGYGRAYREALNGRWGVLDVTDCEDAVR
                     HLVDQGLADPDRIAIRGGSAGGFTTLAALTRSSTFKAGASHYGIGDLNALARDTHKFE
                     SRYLHTLIGSEAALDERSPISHADALSAPVIFFQGSDDRVVPPAQSRQMADALRVRGI
                     PVAYVEFAGEGHGFRRAENIARSLENEHQFFCRVFGLIGDDDVKDLEIENL"
     gene            1331..2290
                     /locus_tag="AC16QF_03945"
     CDS             1331..2290
                     /locus_tag="AC16QF_03945"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR6447734.1"
                     /translation="MSHFELHVAVLMGGPSAEAEVSRVSAAEVAKGLDQAGHRATLLE
                     LDTGAAQALADLTPDVVFPALHGPPGEDGTVQGFLEILNLPYVGSGVHGSAVAMDKSL
                     AKAVFRRAGLPVADDLVVSVGADLAAAEREILDTLGERVVIKPLNQGSAIGVIRVPNG
                     GDLQAALQTALAFGHGVLVEPYVHGREITVGVLDVTGDEPEPFPVIEIITASDEWYDY
                     TNRYTVGKSEHVVPADVPDPVADALREHAVRAHAELGLRDLSRADYILTDANEIVLLE
                     VNTLPGMTPTSLFPDGARATGRDFPTLVDLLVRRAHQRGADGA"
     gene            complement(2339..3616)
                     /locus_tag="AC16QF_03950"
     CDS             complement(2339..3616)
                     /locus_tag="AC16QF_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007942367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR6447735.1"
                     /translation="MKKLMLGLAVLCVSAPAFADDLSQGNTAWILTSTALVLFMTIPG
                     LSLFYCGLVRTKNVLSVLMQCFVITCFVSILWLLVGYSLAFDADGGNAIIGGLGKVLF
                     AGVSESSMSGDIPETAFALFQLTFAIITPALIVGAFAERMRFSAMLMFSGAWLLLVYA
                     PICHWVWGGGWLGDLGLLDFAGGTVVHITAGVAAVVAALTLGSRRGFPQQAMPPHNMT
                     LTIAGAGMLWVGWFGFNGGSALAANGDAAMAMLVTHLSASAGAFTWMCAEWVRYGKPS
                     ALGAVTGMVAGLGTITPASGFVGPAGALVIGILAGIFCFAATNYLKRSLKIDDSLDVF
                     PVHGVGGILGTLLTAVFASSALGVFSGQEDIVILDQLGVQAIGVVATMAWTGIATFVI
                     LKVTDALVGNRVSDEEEVEGLDLVLHNERGYDL"
     gene            3863..4843
                     /locus_tag="AC16QF_03955"
     CDS             3863..4843
                     /locus_tag="AC16QF_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6447736.1"
                     /translation="MQNTEAAVSVEQEKVTTVTDWLSKRIIGQETLLQRLMTALLCGG
                     HLLVEGAPGLAKTRAIKELASAVQGDFHRIQFTPDLLPSDITGTEVYRPEDGSFKFQH
                     GPVFHNLLLADEINRAPAKVQSALLEAMAEHQVSVGRQTFPLPELFLVMATQNPIEQE
                     GTYPLPEAQLDRFLMHVTVSYPSESAETSILHMVRKETGHRDEAATPPFRLTEADIFD
                     ARRAILGLHMAEPVERYIVALIMASREARGELGQWLEFGASPRGTIALDQCARALAWL
                     ERRDYVTPDDVRAIAHDVLRHRLILTFDADAQGVTTDQVIDQLLAEVPVP"
     gene            4906..5784
                     /locus_tag="AC16QF_03960"
     CDS             4906..5784
                     /locus_tag="AC16QF_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6447737.1"
                     /translation="MDLGDLLSIRYRPAHSKTRPARRVSGLAGNRLSKLRGRGIDFSE
                     VRAYQPGDDVRTIDWRVTARKNKPHTKVFREERERLTLVFVDQSQGMFFGSRARLKSV
                     AAAEVAATAAWQALAHNDRVGGIVLGNRDAALHKPRRSVKNVARLLSDLATYNQALSP
                     ATERADPENLRWALTRLRRLAHTGYRIFIISDFEPMGDHWRDALKGLSRHNEVVAVRI
                     FDPLERDLPPPGRYTVTDTEQRWQFHSGNRLLRKTYRERFADHEIELKTICEGSGVRY
                     RSLATDARVGPMAEWA"
     gene            5784..6260
                     /locus_tag="AC16QF_03965"
     CDS             5784..6260
                     /locus_tag="AC16QF_03965"
                     /inference="COORDINATES: protein motif:HMM:NF025674.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HIR6447738.1"
                     /translation="MQPNPLEQLRDIHLPPDPGWWPPAIGWWLLALVIAFVCAAGIRA
                     IYEYRRRRRPVRQARRLYERLSEHLRSGSLSGEAFTHECNELLKRLLVHGFGERDARA
                     ATDETWLAMLDERYGERAFTTGPGRLLGNERFRPDPEFATDELDALMQRFLKRVRP"
     gene            6257..7258
                     /locus_tag="AC16QF_03970"
     CDS             6257..7258
                     /locus_tag="AC16QF_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012075094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6447739.1"
                     /translation="MIEWLWPWAFLLAPLPLAMRLLVSPVARQQAALTVPSVAAFRAT
                     DDSAGTASGRFPWRAVALWLIWLALVTATARPQWTGDPVTLPVTGRDLMLAVDISGSM
                     GTEDLELGGRMVNRLTVVKNVVSQFVDARRGDRVGLILFGTNAYLQAPLTFDLDSVNR
                     LLVEAPVGIAGGKTAIGDAIGLAVKRLRRRPEGERVLILLTDGASNVGEVAPIKAAEL
                     ARQEEIRIHTIGVGADEMRMPSLFGVFGSRVINPSAELDEETLQTIADATGGRYFRAQ
                     NTQRLQEIYTIIDALEPVEQDAETYRPIAALYYWPLGAAWFMSMLLIAGNWRGWGRD"
     gene            7251..8966
                     /locus_tag="AC16QF_03975"
     CDS             7251..8966
                     /locus_tag="AC16QF_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024909.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR6447740.1"
                     /translation="MIESLQFIRPWWLLGLIPAIGLAALWARNRAAGSHWESIIAPEL
                     LNVLLEPTGKGGMRRTAVLAALALAAATVGLAGPTWERLPQPVEQRSDALVVVLDLSL
                     SMYAQDVPPSRIVRARQKITDVLRARDEGFTGLVAYAGDAHSVAPLTDDTETVQNLLA
                     ALSPGMMPVLGSNARSAIDLARQLLDNAGFAQGRILLVTDGVARASDLTDQAHPSYPI
                     SILGVGTASGGTIPLDFVDQPGDVLRTESGEIVRARLDAQYLAALADACHGRYRSMSL
                     GDEDIAFLTATPLPQDAEMLEVEREFDTWADMGYWVCIALIPLALLGFRRGLLAAVCI
                     GILPLPAEANLWDDLWQRRDQQAVEMLEQGDPAAAANLFEDPDWRAAARYRAGDYPGA
                     AEAFAAQLSATGIYNLGNALAHQGDLQTAIAAYSEVLAQQPGHEDAAFNKALLEELLE
                     QQQSQQQQDQNQQDDGQGSEDDQSTPSGSGEQQDQTAEQDPGEQSDRPSGQSASGDEP
                     SEPAPGSETELADARDEQQDALEQWLRRVPDDPGGLLRRKFRYETNQRLRRGEYQSNP
                     SEQIW"
     gene            8963..>9529
                     /locus_tag="AC16QF_03980"
     CDS             8963..>9529
                     /locus_tag="AC16QF_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024973.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HIR6447741.1"
                     /translation="MSRRLIVLLTCLFAPWAWSAIQTSVEPRLLDEMDTLRLTLRSTG
                     QSRADTLDLSPLDEDFEVLGTNTQSQYRSVNGRIDSWVEHQIDLRPRRTGELLVPAIS
                     IGGEQSEAIRIVVRPMDPGVRRAIDRMVFFETELSSNPVYVQGETVFTRRLYYSSGVQ
                     IYSDLPAAPEVPDAVVIPVGETRSSSTLV"
BASE COUNT         1686 a         3178 c         3107 g         1558 t
ORIGIN      
        1 gccagctggt gcgatcggcg ttcgctcccg atggcggcct cgccgacgac gtcgtcatcg
       61 ccggcggtgc ccaggagtcg gtgcagcagc ctcagtggct tggtggcgat gcgctgatct
      121 tcttgagcga ccgcaacggc tactcgaatc tctatcgata cgaccccagc gggctttact
      181 gcacgctgga ggacggtgcg gactacggcg tgccccagtg ggtgttcggc attcgtcagt
      241 tcgccgcggt gagcgaccgg catgtcgtcg ccgcccgcca gggcaccgag acggaactcg
      301 tcatggtcga caccgagggc gggttcgcca cgccgttgac ggacgccggc ggtgctgcga
      361 gcctgggctc ggtggtgaaa tggcaagaca gcgtctgctg cctcgccgag tttcccgacc
      421 ggcttgccgc gatcgtgcgc tgcccgctcg acggctcgcc cccgacggcc attcaccagc
      481 cgggcacggc gccgttgggc gccgagctga tctcccgcgc cgagcgggtt cagtacccga
      541 cccgcgacgg caacagggcc ttcgcctatt actatccacc tcgaaatccc gaacgcccgg
      601 ttacgggcgc cgagggggaa cttccgccgc ttctcgtcat gagccacggt ggccccacgg
      661 cagcggcgtc ccgagcgttg aacctgagaa ttcagtacta cacaacccgc ggctgggcgg
      721 tggtcgacgt ggactaccgc ggcagctccg gatacgggcg agcctaccga gaagcgctca
      781 acggtcgctg gggcgtgctg gacgtcaccg actgcgaaga cgcggtccgc catctggtcg
      841 accaggggct cgcagacccg gaccggatcg ccattcgcgg aggcagcgct ggggggttca
      901 ccacgctggc ggcgctgact cgctcgagca ctttcaaggc cggcgccagc cattacggga
      961 tcggcgatct taacgcgctg gccagggata cgcacaagtt cgaatcacgc tacctgcaca
     1021 ccctcatcgg cagcgaggca gctctggatg aacgctcgcc catctcccat gcggatgcgc
     1081 tgtcggctcc ggtcatcttc tttcagggca gcgacgaccg ggtggtcccg cccgcacagt
     1141 ccaggcaaat ggccgacgcg ctgcgcgtgc ggggcattcc ggtggcctac gtcgagtttg
     1201 ccggcgaagg ccacggcttc cgacgggccg aaaacatcgc caggtcgctc gagaacgagc
     1261 accagttctt ctgccgcgta ttcggtctca ttggtgatga cgacgtaaag gatttggaga
     1321 tagagaacct gtgagccatt tcgaactgca cgttgccgtt ctcatgggcg gaccatctgc
     1381 cgaagccgaa gtttccaggg tatccgccgc ggaggtcgca aagggcttgg atcaggccgg
     1441 tcaccgggca acccttcttg aactggatac cggagcggcg caggccctcg ccgacctgac
     1501 tccggacgtg gtgtttcccg ctcttcacgg cccgccgggg gaagatggca ccgttcaggg
     1561 cttcctggaa attctcaatc tgccctacgt cggcagcggc gttcacggca gcgccgtggc
     1621 catggacaaa agcctggcca aggccgtgtt tcgccgtgcg ggcctgccgg tggccgacga
     1681 cctcgtcgtg agcgtcgggg ccgatctggc ggccgccgag cgggagattc tggacacgct
     1741 cggggagcgc gtagtgataa agcctctgaa tcagggctcc gccatcggcg tgatccgcgt
     1801 gccgaacggc ggcgatctgc aagccgcgct gcaaacggcc ctggcgttcg ggcatggggt
     1861 cctcgtcgag ccctacgtcc atggccgcga gatcacggtc ggcgtgctgg acgtcaccgg
     1921 ggacgagccc gagccgtttc ccgtgatcga gatcatcacc gccagcgacg agtggtacga
     1981 ctacaccaac cgctacaccg tgggaaagag cgagcacgtg gttccagccg acgtgccgga
     2041 cccggtggcc gacgctctga gagagcatgc cgttcgcgcc cacgcggagc ttggcttgag
     2101 ggacctgtcg agagccgatt acatcctcac ggacgccaac gagatcgtcc tcctggaggt
     2161 gaacacgctg cctgggatga cgccgaccag ccttttcccg gacggcgccc gggccaccgg
     2221 aagggatttc cccacgctgg tcgacctcct ggtccgcagg gcccaccagc ggggcgcaga
     2281 cggagcctga ccgccccctt gggaaatgac cctgaaaact tagatagttt tcagggtgtt
     2341 acaggtcata tccacgttcg ttgtgaagga cgagatcgag cccttccacc tcttcctcgt
     2401 cggagacgcg gttcccgacc agcgcgtcgg tcaccttgag aatcacgaac gtcgctatgc
     2461 ccgtccacgc catggtggcg accacgccga tggcctgaac gccgagctgg tcgaggatca
     2521 cgatgtcctc ctgcccgctg aagacgccca gggcgctgct ggcgaaaacg gccgtgagca
     2581 gcgttcccag aatcccgccg accccgtgga cggggaacac gtccagcgag tcgtcgatct
     2641 tcaatgaccg cttgaggtag ttggttgcag cgaagcagaa tatcccggcg agaatgccga
     2701 tgacgagcgc gccggcggga ccgacgaagc ccgatgccgg cgtgatggtc ccgagcccgg
     2761 cgaccatacc cgtcacggcg cccagcgcgc tgggcttgcc gtatctgacc cactcggcgc
     2821 acatccaggt gaacgcgccg gcggacgcgg acagatgggt gaccagcatg gccatcgcag
     2881 cgtcgccgtt ggctgccagg gcagacccgc cgttgaatcc gaaccacccc acccagagca
     2941 tcccggcccc cgcaatggtg agcgtcatgt tgtgcggcgg catggcctgc tgcgggaaac
     3001 cgcggcggga cccgagcgtg agcgctgcga ccaccgcggc aacgccggcg gtgatgtgga
     3061 ccacggtgcc gccggcaaaa tccaggagcc cgaggtcgcc gagccagccg ccgccccaga
     3121 cccagtgaca tatcggagcg tatacgagca gcagccaagc accgctgaac atcagcatcg
     3181 cagagaagcg catccgctcg gcgaaggcgc ccacgatgag cgcgggggtg atgattgcaa
     3241 aggtaagctg gaagagcgcg aaggccgtct ccgggatgtc tcccgacatg gaactctcag
     3301 acaccccggc gaacaggacc ttgcccagcc cgccgatgat ggcgttgccg ccgtcggcgt
     3361 cgaacgccag gctatatccc accagcaacc agaggatgga cacgaagcac gtgatgacga
     3421 agcactgcat gagcacgctg agcacgttct tggtccggac gagcccgcag taaaagaggg
     3481 aaagaccagg aatggtcatg aacagaacca gcgccgtcga cgtgaggatc caggccgtgt
     3541 tgccctggct caggtcgtca gcgaacgcgg gcgccgacac acacagcacg gccaacccca
     3601 acatcagttt tttcatcgat agtcccctct tatgtcgcca cccgcccggc tttctgtgtc
     3661 tccggttccc ggatgcccgc acaccgcttg atttcatcaa cgaatttact tcgatttcgc
     3721 caggggtgcc agaggcgatc cgcgtgctgc tcgattgacg cggaccggaa cgccggcgtc
     3781 gaggatgtat ttcgttgccg gaaggctaca atacccggcc cggacgcccc ggcgggcttg
     3841 cccgggcgcg catgaggacg atatgcagaa cacggaagca gcagtttcgg tcgaacagga
     3901 gaaggtcact acggtgaccg actggctatc taagcgcatc atcgggcagg agaccctgct
     3961 ccagcgtctt atgaccgcgc tcctttgcgg cggccatctg ctggtagaag gcgcgccggg
     4021 gctggccaag acccgcgcca tcaaggaact tgcaagcgcc gttcagggcg attttcatcg
     4081 catccagttc acgcccgatc tgctgcccag cgacatcacc ggaaccgagg tgtaccgccc
     4141 ggaggacggc tcgttcaagt ttcagcacgg tcccgttttc cacaatctgc tgcttgccga
     4201 cgagatcaac cgtgccccgg cgaaggttca atcggcgttg ctggaggcga tggccgagca
     4261 tcaggtcagc gttgggcgtc agacgttccc cctgcctgaa ctttttctcg tcatggcgac
     4321 ccagaacccc atcgagcagg agggcaccta cccgctgccc gaggcccagc tcgaccgctt
     4381 cctgatgcac gtgacggtca gctacccctc ggaatccgcg gagacgtcca ttctccacat
     4441 ggtccgaaag gaaaccggac acagggacga ggcggcaaca ccgcccttcc ggctgaccga
     4501 agccgacatc ttcgacgcgc gccgcgcgat actcggtctg cacatggccg aacccgtgga
     4561 gcgctacatc gttgcgctga tcatggcgtc gcgggaagcg cgcggagagc tgggccaatg
     4621 gctggaattc ggcgcaagcc cacggggcac catcgcgctg gaccagtgtg cccgggcact
     4681 ggcctggctg gaacgcagag actacgtcac acccgacgac gtgcgcgcga tcgctcacga
     4741 cgttctgcgg catcggttga tcctcacctt cgacgcggac gcccagggcg tcacgacgga
     4801 ccaggtcatc gaccagttgc tcgccgaggt gccggttccc tgagctcgcc gactgctagc
     4861 acgatcctcg gctcgggtcg ggaagaaatc ctgaccggcg cctacgtgga cctcggcgac
     4921 cttctgtcca tacgctaccg gccggcccac tcgaaaacgc gaccggcgcg gcgggtcagc
     4981 gggctggcgg gcaaccgcct gtccaagctg cggggccggg gcatcgactt ctccgaggtg
     5041 cgtgcctacc agcctggcga cgatgtacgc accatcgact ggcgggtcac tgcgcgcaaa
     5101 aacaagcctc acaccaaggt atttcgagag gaacgcgaaa ggctcaccct ggttttcgtc
     5161 gatcagagtc agggcatgtt cttcggcagc cgcgcccggc tcaaatccgt cgctgccgcc
     5221 gaggttgccg ccaccgccgc ctggcaggcg ctcgctcaca acgatcgcgt cggcggcatc
     5281 gtgctgggca accgggacgc cgccctccac aaaccccggc gaagcgtcaa gaacgtggcc
     5341 cgcctgctgt ccgatctggc cacctataat caggcgctct cccccgcgac ggagcgcgca
     5401 gaccctgaaa atctgcgctg ggcgctgacg cgcctgcggc gcctggccca cacgggttat
     5461 cggatattca tcatcagcga cttcgaaccc atgggcgatc actggcgcga tgcgctcaag
     5521 gggctgtcgc gccacaacga ggtggtcgcc gtccggatat tcgacccgct ggagcgagac
     5581 ctgccgccac caggccgcta tacggtgacc gataccgagc agcgctggca gtttcacagc
     5641 ggcaaccggc tgcttcgcaa gacctatcgg gaacgcttcg cggatcacga gatcgaactg
     5701 aagaccatct gcgagggctc cggcgtccgc taccgttcgc tggcgaccga cgcgcgggtg
     5761 gggccaatgg ccgagtgggc gtgatgcagc cgaatcccct ggagcagctc agagacatcc
     5821 atctgccccc ggaccccggg tggtggccgc cggccatcgg ttggtggctg ctggcattgg
     5881 tgatcgcttt tgtctgcgcg gccggcatcc gggccatcta cgagtatcgg cgccggcgcc
     5941 gtcccgtccg gcaggcccgg cgtctctacg agcgtctcag cgagcacctc aggtcggggt
     6001 ccctttccgg cgaggccttc acccacgagt gcaacgagct tttgaagcgg ttgctcgttc
     6061 acggcttcgg cgagcgtgac gccagagcgg ccacggacga aacctggctg gcgatgctgg
     6121 acgaacgtta cggcgaacgg gcgttcacca ccggtccggg ccggcttctc ggcaacgagc
     6181 gctttcgccc cgacccggag tttgccaccg acgaactcga cgcgctgatg cagcgcttcc
     6241 tgaagcgggt gcggccatga tcgagtggct gtggccgtgg gccttcctct tggcgccact
     6301 gcccctggca atgcggctgc tcgtcagccc cgtggcgcgt cagcaggcag ctctaacggt
     6361 gccaagcgtc gcagctttcc gcgccacgga cgatagcgcc ggcacggcca gcggacgctt
     6421 cccctggcgc gcggtcgcgc tgtggctgat ctggttggcg ctggtcacgg ccaccgcaag
     6481 gccgcaatgg acgggcgatc cggtcacgct tccggtaacc ggaagggacc tcatgctggc
     6541 cgtggacata tccggaagca tgggcaccga ggatctggag ctgggcgggc gaatggtcaa
     6601 tcgcctgacg gtggtcaaga acgtcgtcag ccagttcgtg gacgcccggc gcggtgaccg
     6661 cgtcgggctg atcctgttcg gcaccaatgc ctacctccag gcgccgctca cgttcgacct
     6721 ggacagcgtc aaccgcctgc tggtggaagc tcccgtgggc atcgccggcg gcaagacggc
     6781 gatcggtgac gccatcggtc tggccgtcaa acggctgcgc agaagacccg agggcgaacg
     6841 cgtgctcatc cttcttaccg atggggcgag caacgtcggc gaggtcgcgc cgataaaggc
     6901 ggcagagctc gctcgccagg aggagatccg catccacacc atcggcgtgg gcgcggacga
     6961 gatgcggatg ccgagccttt tcggcgtctt tgggtccagg gtgatcaacc cctcggcgga
     7021 gctcgacgag gaaaccctgc agaccatcgc cgacgccacc ggagggcgct acttccgcgc
     7081 ccagaacacc cagcgccttc aggaaatcta caccatcatc gacgcgctcg agcccgtgga
     7141 gcaggatgcc gagacctacc ggcccatcgc cgcgctctac tactggccgc tgggcgccgc
     7201 ctggttcatg tcgatgctgc tgatcgccgg caactggcga ggctggggcc gtgattgagt
     7261 cattgcaatt catccgcccg tggtggctgc ttggcctgat tcccgccatt ggcctggctg
     7321 cgctgtgggc ccgaaaccgc gccgcggggt cgcactggga gtccatcatc gccccggagc
     7381 ttctcaacgt tctgctcgag ccaaccggca aggggggcat gcggcgaacg gccgtcctcg
     7441 cggcattggc gctggcggca gcaaccgtcg gtctggcggg gccgacgtgg gaacgcctgc
     7501 ctcaacctgt ggagcagaga agcgacgctc tggtggtcgt gctcgatctc tcgctgtcca
     7561 tgtacgcgca ggatgtgccg ccctcgcgca tcgttcgcgc tcgccagaag atcacagacg
     7621 tccttcgggc ccgggacgag ggtttcaccg gcctggtcgc ctacgcggga gacgctcact
     7681 cggttgcacc gctgaccgat gacaccgaga ccgtccagaa cctgctggca gctttgagcc
     7741 cgggcatgat gcccgtcctc ggcagcaacg cccggtcggc catcgacctt gcgcggcagc
     7801 tcctggacaa tgccggtttt gcgcagggac ggatcctgct ggtcaccgac ggcgtcgcgc
     7861 gcgcctcgga tctcaccgac caagctcacc ccagctatcc catctccata ctcggcgtgg
     7921 gcaccgcctc gggcggcacg atccccctgg atttcgttga tcagccggga gacgtgctgc
     7981 gaaccgaatc gggagagatc gtccgcgccc gcctggatgc ccagtatctt gccgcccttg
     8041 ccgacgcctg ccacggccgt tatcggtcca tgagtttggg cgacgaggac attgcctttc
     8101 tcacggcgac cccgctgccc caggacgccg aaatgcttga ggtggaacgg gagttcgaca
     8161 cgtgggcgga tatgggttac tgggtctgca tcgcgctcat cccgctcgcg ctgctcggtt
     8221 tccggcgcgg tctgctagcc gccgtgtgca ttgggatcct cccgctgccc gccgaggcaa
     8281 acctctggga cgatttgtgg caacggcggg atcagcaggc cgtggagatg ctcgagcagg
     8341 gcgacccggc ggccgctgcg aacctgttcg aggatcccga ctggcgcgcc gccgcccgat
     8401 accgggcagg cgactaccca ggggcggcgg aggcgtttgc cgcccagctg tcggcaacgg
     8461 gcatctacaa cctgggcaac gccctggcgc accaggggga tctgcagacc gccatcgccg
     8521 cctacagcga ggtgctggcg cagcagcccg gccatgaaga tgcggccttc aataaagccc
     8581 tcctcgagga gttgctggag cagcagcaat cgcaacagca gcaggaccag aaccagcaag
     8641 acgacgggca aggatcagaa gatgaccagt ccacgccctc cggttccggt gagcagcagg
     8701 atcagaccgc agagcaggat cctggggagc agagcgatcg gccttcgggg caatcggcgt
     8761 ccggcgacga gcccagcgag ccggctccgg ggtcggagac ggaactggct gacgctcgcg
     8821 atgagcagca ggatgcgctg gagcagtggc tgcgccgggt gcccgacgat ccgggcggcc
     8881 ttctgcgccg gaagttccgc tacgaaacca atcagcgact gcgcagaggc gagtaccaaa
     8941 gcaacccctc ggagcagata tggtgagccg gcgactcatc gtcctgctca cctgcctgtt
     9001 cgcaccttgg gcatggtcag ccatccagac ttccgtggag cctcggctgc tcgacgagat
     9061 ggataccctt cggctgactt tgcgctccac aggccagagc cgggcggaca cattggacct
     9121 gtcgccgctc gatgaggatt tcgaggtgct cggcaccaat acccagagcc agtaccgctc
     9181 cgtaaacgga cgcatagaca gctgggtcga gcaccagatc gatctacggc cacgtcgaac
     9241 gggcgagttg ctggtgccgg cgatctccat cggcggcgag cagagcgagg cgatccgaat
     9301 cgtggtgcgt cccatggacc cgggcgtgag gcgtgccatc gatcgcatgg tcttcttcga
     9361 gaccgagctg tccagcaacc cggtatacgt tcagggcgag accgtgttca cgcgcaggct
     9421 ctactactcg agcggcgtgc agatatacag cgacctgccc gctgccccgg aagtgccgga
     9481 cgcggtggtc ataccggtgg gcgagacgcg atcgagcagc actctcgtg
//
LOCUS       DBNHLM010000082        10901 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1148994, whole genome shotgun sequence.
ACCESSION   DBNHLM010000082 DBNHLM010000000
VERSION     DBNHLM010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10901)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10901)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10901
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1148994"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="AC16QF_03985"
     CDS             complement(<1..857)
                     /locus_tag="AC16QF_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR6447742.1"
                     /translation="MYHVLLVDSGNRRETVRGLLENAGITVVGPESIEALRQIDARQY
                     RCLLAAVGPLFGTTEDAIDLAGRLPVVLLDPVGDARRAVAAMKRGAADYLVCPFTEEE
                     LLSAVERCRPTGAPQPRDPLNMVGASQPMKDLYNRIAQVAPTDSTVLIEGESGSGKEL
                     VARAIHAASRRNDAPLITLNCATVPAELIESELFGRPAEGQAASRHGLLEAANGGTLF
                     LDEIAELPPNVQARLLRVLQDGCLGLSSDGTPFTVDVRIIAATHQNLRQLIEADQFRE
                     DLYYRLNVV"
     gene            complement(913..1770)
                     /gene="gluQRS"
                     /locus_tag="AC16QF_03990"
     CDS             complement(913..1770)
                     /gene="gluQRS"
                     /locus_tag="AC16QF_03990"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009050893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HIR6447743.1"
                     /translation="MAGRFAPSPTGPLHLGSLLAATASYLDARAAGETWRLRFDDLDV
                     PRNDPGAIGSITATLRAHGLIWDGPIVHQSDNLTAYEEALNRLDRMGLLFYCTCSRRD
                     LRAEAVYPGTCRAFREPRRDSATRVLVDQVAIEFADRIQGPQRCRLDQTVGDFIVRRR
                     DGLVAYQLATAVDDGGEEVTSVVRGADLLDNTPRQIYLMQRLGLKPPRYAHIPVLLKP
                     DGSKLSKQTMAPPVDAHRAPENLLTAYGLLGMRPPSDAQRWEVERILSWGISHWDQEK
                     VPRRRSVEP"
     gene            complement(1806..2267)
                     /gene="dksA"
                     /locus_tag="AC16QF_03995"
     CDS             complement(1806..2267)
                     /gene="dksA"
                     /locus_tag="AC16QF_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HIR6447744.1"
                     /translation="MAARAKNRSTKAKAVDSPFRNFTPYKPRKSEAYMSDGQVEHFRN
                     ILSKWRQELMEEVDRTVHHMQDEAANFPDPADRATQEEEFSIELRTRDRERKLIRKID
                     QTLDRLDQGDFGYCDTCGVEIGLRRLEARPTANQCVDCKSLDEIKERQLHG"
     gene            2431..3603
                     /locus_tag="AC16QF_04000"
     CDS             2431..3603
                     /locus_tag="AC16QF_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007159703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6447745.1"
                     /translation="MYAQRLDRIAPFRVMQLMERAKAIEASGHRVVHFEVGEPDFVTA
                     DPIVRAGIDALEAGETKYTQALGIPELRERIAQHYCQELGVSLSADRVVVTSGASGGL
                     LLLSGLLLNPGDRLLLTDPGYPCNEVFAGLVNAEPERIPLSADRGFQLHADDLEAHWG
                     PRARGLLLASPANPTGAVISRAHLAELVAALARRGGWLILDEIYQGLIYGDGPGYRTG
                     LEITDEAFILNSFSKYYGMTGWRLGWMVVPEAALEPVARLAQNLFISPSSIAQRAALS
                     AFGPEARAIHEQRRAAFEGRRDALREGLTELGLRVPVVPGGAFYLYVDVSPIGIDSAE
                     FCWRLMDDYHVAVTPGIDFGDHLADQYVRFAYTTDEDSIALGLERIETALKDMGAL"
     gene            3600..4301
                     /gene="sfsA"
                     /locus_tag="AC16QF_04005"
     CDS             3600..4301
                     /gene="sfsA"
                     /locus_tag="AC16QF_04005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HIR6447746.1"
                     /translation="MNFHPPLEVARLERRYKRFLADLLTDQGPLTVHCPNTGAMLGCS
                     DPGSEVWYSDSGNERRKYRHTLEVVCTPTGRVGINTARANRLVEEAIARGGVAELDGY
                     GQIRREAAIPESKGRFDFLLEGARGRCFVEVKSMTHNLGRGRGAFPDTVSERALRHVE
                     ALIGRKRAGDRAVLLFCVQHTGIDLATTADAIYPEYGEAVRRAVSEGVEVIAYGCRID
                     RDALAVGEALPVELS"
     gene            complement(4294..5289)
                     /locus_tag="AC16QF_04010"
     CDS             complement(4294..5289)
                     /locus_tag="AC16QF_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HIR6447747.1"
                     /translation="MYDIYGLGNALVDMEYRVDDGFLRRHAITKGHMTLVEEDRLDAL
                     IADLDDYDPERMSGGSAANTVIAAQCFGVSTFYSCKVAGDATGHYFLRDMGTAGVNTN
                     ANANDGPGKSGRCLVLVTPDAERSMNTFLGVSSQLSTAEIDEAALAKAGFFYVEGYLS
                     SSPGSLEAAVECRQLAEDSGVKTAVSLSDPSMVEFFRDGMEQILGNGVDHLFCNEEEA
                     LAWSRTDRLDIAIRELAEVARAVNITLGARGSLAVNGREQRRAPGYPVTAVDTNGAGD
                     IYAGACLFGWAAGMSSEQAAAFGNFAAANLVQRYGARFRHAGDYQSVLGDFRKVS"
     gene            complement(5304..6584)
                     /gene="hemL"
                     /locus_tag="AC16QF_04015"
     CDS             complement(5304..6584)
                     /gene="hemL"
                     /locus_tag="AC16QF_04015"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HIR6447748.1"
                     /translation="MHTDRSRELMRRAALSIPGGVNSPVRAFKGVGGSPIFFERGEGA
                     FLTDVDGNRYIDYVGSWGPMLLGHGNPEVVAAVRAQAEKALGFGAPTELEIELAEQVV
                     DMVPSIEKIRMVNSGTEATMSAIRLARGFTGRDLIVKFDGCYHGHSDSLLVKAGSGVL
                     TLGIPNSPGVPAAIAEQTLTLPFNDLDAVAEAFEQYAGQIAAVILEPVAGNMGCVPPE
                     AGYLEGLRELTGRHGSLLIFDEVMTGFRVSRGGAQQRYGVEPDLTTLGKIIGGGLPVG
                     AFGGRADVMAHIAPEGTIYQAGTLSGNPLAMVAGLTTLRALDDPLYEELEQRTAYFCE
                     ALEASANRHGVSVTINYVCGMFSVFFTNGPVRNFADVARSDTASFNRFFHAMLDGGVY
                     LAPSAFEAGFLSVAHTEDVLDATIQAADQAFATL"
     gene            complement(6612..7046)
                     /gene="hemJ"
                     /locus_tag="AC16QF_04020"
     CDS             complement(6612..7046)
                     /gene="hemJ"
                     /locus_tag="AC16QF_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007251893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HIR6447749.1"
                     /translation="MSAGYLWLKALHIIFVVTWFAALFYLPRLFVYHSSSTDAISRDR
                     FKVMESKLYRVIMRPSMLLATGFGLWLVFLGWNAFAGSGWLWVKLTAVVALLGYHHYC
                     ARLVRAFAADADPHSETFYRWFNELPALVLIVVVIMVVVKPF"
     gene            complement(7055..8836)
                     /locus_tag="AC16QF_04025"
     CDS             complement(7055..8836)
                     /locus_tag="AC16QF_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIR6447750.1"
                     /translation="MIRFTSSSSAPSAEIGALRLPNKVSPEFFRNRLADIEALPQLAL
                     LAVLAGLITGCVILLFRAAVELILGGWLLPEGSESFEALSILERLALPIAGAGLIGLA
                     LNRLSAAERRVGVVHVMERLSRHQGYLPWRNAAVQFFGGIAALASGQSGGREGPAIHL
                     GAASSSLIGQIFRLPNNSLRTLVACGSAAAIASSFNTPIAGVIFAMEVVMMEYTIGSF
                     IPVIIAAVTSTLLTHYFIGDAPAFTVAPLQMQSLSEVPFVVLAGIVIGCTAAGFTALV
                     QLFARLSRWPFWSRALLAGALTGLAATMVPAVMGIGYDTVNAAMLGQLSLTALLLVAL
                     FKSISSAAAVGLGLPVGLIGPTLVIGAALGGCIGMIGNLLEPADAISEGFYVMLGMAA
                     MMAAVLQAPLAALMAVMELTLNPNLILPAMLMIVVATLVSSVAFRQRSVFISTLKTLG
                     LEYPPSPVTAHMQRAGVAAIMCEEVAVLPRDCSLDAARRALEQRPLWIAIASDDGETR
                     WVLKSSDLAAHLESEQIDTADVDLLNIPGMRRDATSVDVRCTVDEAQMALQGSPAEAL
                     CVRRLGVTAAGSLLGIVTQEDIDTYRG"
     gene            8877..9635
                     /locus_tag="AC16QF_04030"
     CDS             8877..9635
                     /locus_tag="AC16QF_04030"
                     /inference="COORDINATES: protein motif:HMM:NF042896.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HIR6447751.1"
                     /translation="MARDPLKGIIKGEGQVRVVAHSPRRRLLTSLTVALVCAAGAAGG
                     FYLGESKAGLDAGYIVALEKMDRANAVEIAELRSRLIDANLARSVESQAARELRDDIR
                     ILRDEVAGLQEEVTFYKSIMAPDSIERGLQIAEFQLVPGEAVNEFTYHLLLTQVEARR
                     DWIQGTVTLDVVGRRTPDPSRKDVELVLSLTEIGQTDTYPLSFRFRYFQDLTGSMALP
                     DGFLPEAVVVTARKRGARSEQLQRKFAWAMGEAE"
     gene            9697..10146
                     /locus_tag="AC16QF_04035"
     CDS             9697..10146
                     /locus_tag="AC16QF_04035"
                     /inference="COORDINATES: protein motif:HMM:NF016408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR6447752.1"
                     /translation="MARTKVRDGVTLIAANTEVTGNVKFNGQLYVSGRVNGNILAAEG
                     CEATVVVSEEGVVSGEIRVPNVVINGTVEGDISASERIELACKARVKGSVYYALVEMQ
                     LGAQVDGQLVHEHPQTAASEDAHKAGAKRRNPLGIIKQLPKAGATAS"
     gene            10261..10605
                     /gene="erpA"
                     /locus_tag="AC16QF_04040"
     CDS             10261..10605
                     /gene="erpA"
                     /locus_tag="AC16QF_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009408529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HIR6447753.1"
                     /translation="MTGQLDIDISFTERAAAKVRALIADEENADLKLRVFITGGGCSG
                     FSYGFTFDEEVAEDDAVVEREGVSMLVDSLSYQYLLGAEVDYKEGLQGAQFVVQNPNA
                     SATCGCGNSFAI"
     gene            complement(10624..>10901)
                     /locus_tag="AC16QF_04045"
     CDS             complement(10624..>10901)
                     /locus_tag="AC16QF_04045"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HIR6447754.1"
                     /translation="SITDAVDRWGGRAERWLVCGGGRHNRLLMERLAQLTPSPIETTD
                     AHGVDGDALEAGAFAWLAHQTMASLPGNAPGVTGASGERILGGIYRP"
BASE COUNT         1915 a         3439 c         3637 g         1910 t
ORIGIN      
        1 ttcacgacgt tcaggcgata gtagagatcc tcccggaact ggtctgcctc gatgagctgt
       61 ctcaggttct ggtgggtcgc ggcgatgatg cgcacgtcca cggtgaaggg ggtaccgtcg
      121 ctggacaggc ccaggcagcc gtcctgaagc actcgcagca gacgcgcctg aacgttcgga
      181 ggcagctcgg cgatctcgtc cagaaacagg gtcccgccgt tggccgcttc caaaagcccg
      241 tggcggctgg ccgcttgccc ctcggctggt ctgccgaaca gctcggactc gatcagctca
      301 gccggcacgg tggcgcagtt cagcgtgatc agcggcgcat cgttgcggcg gcttgccgcg
      361 tggatcgcgc gcgctacgag ctcctttccg gagccggact ctccctcgat gagcaccgtt
      421 gagtcggtgg gcgcgacctg ggcgatccgg ttgtacagat ccttcatcgg ctggctcgcg
      481 cccaccatgt tcagcgggtc acgcggctgg ggggcgcctg tggggcggca gcgctctacg
      541 gcgctcagga gctcctcttc cgtgaagggg cagaccaggt agtcggcggc gccgcgcttc
      601 atggctgcca ccgcccggcg agcgtcgcct acgggatcca ggagcaccac ggggagcctg
      661 ccggccaggt cgatggcgtc ttcggtcgtt ccgaacagag ggcccacagc ggcgagcagg
      721 caccggtact ggcgggcatc gatctggcgg agcgcttcga tgctctccgg ccctaccacc
      781 gtgatgccgg cgttctcgag aaggcctcga acggtctcgc gacgattgcc cgaatccacc
      841 agaagtacgt gatacatggc gcgcgatggt aacaaaaagg tcagggtgcg cgcgcgaccc
      901 tgcagtgggg cgtcaaggct cgacggaccg ccgacgtggg accttctcct ggtcccagtg
      961 gctgatgccc caggagagaa tgcgctcgac ttcccaccgt tgcgcatcgg aaggcgggcg
     1021 cataccgagc aggccgtacg ccgtcagcag gttctcgggc gcccgatggg cgtccacggg
     1081 cggggccatg gtctgcttgg acagcttgct gccgtcgggt ttcaggagga cgggaatgtg
     1141 cgcgtagcgc ggcggcttca gaccgagccg ctgcatcagg tagatctggc ggggcgtgtt
     1201 gtccagcagg tcggcgcccc tgaccaccga agtaacctct tcgcccccgt catccaccgc
     1261 ggtggccagc tggtaggcca ccagaccgtc gcgtcggcgc acgatgaagt cgcccaccgt
     1321 ctggtcgaga cgacagcgtt gcgggccttg tatgcggtcg gcgaactcga tggccacctg
     1381 gtccaccagc accctggtgg cgctgtctcg tcggggctcg cgaaaggcgc ggcacgtgcc
     1441 ggggtaaacc gcctcggcgc gcaggtcgcg cctggaacag gtgcagtaga acagcagccc
     1501 catgcggtcg agacggttca gcgcctcttc gtatgccgtc aggttgtcgc tctggtggac
     1561 gatgggcccg tcccagatca ggccgtgagc cctgagcgtg gcagtgatgc tcccaatggc
     1621 gccgggatcg tttctgggaa cgtccagatc gtcgaagcgc agccgccagg tttcgccggc
     1681 ggcacgggca tccaggtagc tggcggtcgc ggcgagaagg gatccgaggt gcagcggacc
     1741 ggttggtgac ggcgcgaacc gaccggccat gccttgcgaa gatgcgcggg agacggagcg
     1801 tcccgtcagc cgtgcagctg cctctccttg atctcgtcga gggacttgca gtcgacgcac
     1861 tggttggcgg tgggtcgtgc ctccaagcgg cgcagaccga tctcgacccc gcaggtgtcg
     1921 cagtagccga agtcaccctg gtccaggcga tcgagggtct ggtcgatctt cctgattagc
     1981 ttgcgctccc tgtctcgggt ccggagctct atgctgaatt cctcttcctg ggtcgcgcgg
     2041 tccgccggat ccgggaaatt ggccgcttcg tcctgcatgt ggtggacggt tcgatcgacc
     2101 tcttccatca gctcctgtcg ccacttggag aggatgttgc ggaagtgctc gacctggccg
     2161 tcgctcatgt acgcttccga cttcctgggt ttgtagggcg tgaagttgcg aaatggagaa
     2221 tcgactgcct tggctttcgt agatcgattt tttgcgcggg ccgccatatg tcgcctctga
     2281 tcgtcctttc caatggaaaa tcggctgatt tttcgagccg gatttcgcga agggcgcgta
     2341 agctaccaga agaaatcggc gttgaccata taatccgtcc aggtttttcg tgatttctga
     2401 cgcacggctc aaagggagaa ttcagggcac atgtacgcgc agcggctgga ccgaatagcc
     2461 ccgtttcggg tcatgcaatt gatggaacgg gcgaaggcca tcgaggcttc ggggcaccgc
     2521 gtcgtgcact tcgaggtagg cgagccggac ttcgtgaccg ccgaccccat cgttcgggcg
     2581 ggcatcgatg ccctggaggc aggggaaaca aagtacaccc aggcgctggg catcccggag
     2641 ctgcgtgagc gcatcgccca gcattactgt caggaacttg gtgtttctct ctccgccgac
     2701 cgggtcgtgg tcacctcggg cgccagcggt ggtctgctcc tgctgagcgg gttgctgttg
     2761 aacccgggtg accgcctgct gctcaccgac cccggatatc cgtgcaacga ggtgttcgcc
     2821 gggctcgtca acgccgagcc cgagcgaatt ccgctgtccg ctgaccgtgg ctttcagctg
     2881 cacgctgacg atctggaggc gcattggggg cccagagcgc ggggtttgct gctggcttcg
     2941 ccggcaaacc ccacgggggc ggtgatctcc cgggcgcatc tcgcggagct cgttgctgcc
     3001 ctggctcgta gaggcggctg gctgatcctc gacgagatct accagggcct gatctacggt
     3061 gacgggcccg gataccgcac cggtcttgag atcaccgacg aggcattcat tctcaacagc
     3121 ttttccaagt actacggaat gaccggctgg cggctcgggt ggatggtggt tccggaggcc
     3181 gcgctggagc ccgttgccag gctcgcccag aatctcttca tctcgccgtc gtccatcgcc
     3241 cagcgggcgg cattgtcggc cttcgggccg gaggcgcggg cgattcacga acaacgacgt
     3301 gctgccttcg aggggcgcag agacgcgctg cgcgagggcc tgacggagct cggtctgcga
     3361 gtccccgtgg tgccgggcgg ggcgttctat ctctacgtgg atgtctcgcc catcggcatc
     3421 gactcggccg agttctgctg gcgcctgatg gacgattacc acgtagcggt tacgccgggg
     3481 atcgattttg gtgaccatct ggccgatcag tacgtgcggt tcgcgtacac caccgacgaa
     3541 gactccatcg ccctcgggct cgagcggatt gagacggcgc tcaaggacat gggcgccctg
     3601 tgaatttcca cccgccgctg gaagttgctc gcctggagcg tcgctacaaa agatttctcg
     3661 ccgacctgtt gaccgaccag ggtccgctga ccgtgcactg ccccaacacc ggcgccatgc
     3721 ttggatgctc agatcccggc agcgaggtct ggtattcaga ctccgggaac gagcgccgca
     3781 agtatcgtca cacgctcgag gtggtgtgca cgccgaccgg ccgggtgggg atcaacacgg
     3841 caagagccaa tcggctggtc gaagaagcca ttgcccgggg cggcgtggcc gagcttgacg
     3901 ggtacgggca gatcaggcgc gaagcggcca tccctgaaag caagggtcgc ttcgatttcc
     3961 tcctcgaggg cgccaggggg cgctgcttcg tcgaggtcaa gagcatgacc cacaatctcg
     4021 gccgggggcg cggcgccttc ccggatacgg tgagcgaacg ggcgttgcgc cacgtcgaag
     4081 cgctgatcgg caggaagcgg gccggtgacc gcgccgtgct gctgttctgc gtccagcaca
     4141 ccggcatcga cctggcgacc acggcggatg cgatctatcc cgagtacggt gaagcggtgc
     4201 gtcgggccgt ctccgagggt gtcgaggtga tcgcctacgg gtgtcggatc gatcgggatg
     4261 cccttgcggt gggcgaggcg ctccccgtgg agctcagctg accttccgga agtcgccaag
     4321 cacgctctgg tagtcgcctg cgtgcctgaa tcgggcaccg taccgctgta ccaggttagc
     4381 cgccgcgaag tttccgaagg cggccgcctg ctccgaggac atgccggccg cccagccgaa
     4441 caggcaggca ccggcgtaga tgtctccggc accgttggtg tcgacggccg tcaccgggta
     4501 gcccggcgcg cggcgctgct cacggccgtt cacggccagg ctgccccggg cgcccagggt
     4561 gatgttcact gcccgggcca cctcggcgag ctcgcggatg gcgatgtcca gccggtccgt
     4621 tcgcgaccac gccagtgctt cttcctcgtt gcagaacaaa tggtccacgc cgttgcccag
     4681 aatctgctcc atgccgtccc tgaagaactc gaccatgctg ggatcggaga ggcttaccgc
     4741 ggttttcacg cccgagtcct cggcaagctg ccggcactcc acggccgcct ccaggcttcc
     4801 cggactggag ctcaggtagc cctcgacgta gaagaagcct gctttggcca gtgccgcctc
     4861 gtcgatctcg gctgtgctga gctggctcga cacgccgaga aaggtgttca tggatcgctc
     4921 cgcgtcgggt gtgaccagca ccaggcaacg ccccgacttg cccggtccgt cgttggcatt
     4981 ggcgttggtg ttcacgcccg ccgtgcccat gtcccgcaga aagtagtggc cggtggcgtc
     5041 gccggcgacc ttgcaggagt agaaggtgct tacgccgaag cactgggctg cgatgaccgt
     5101 gtttgccgcg gatccaccgc tcatgcgttc ggggtcgtag tcgtccaggt cggcgatcag
     5161 agcatcgagc cggtcttcct ccaccagcgt catgtgtcct ttggtgatgg catgacggcg
     5221 cagaaaaccg tcatccacgc gatactccat gtccaccagg gcatttccca ggccgtagat
     5281 gtcatacatg aacggggctc cccttacagc gttgcgaacg cctgatctgc ggcctggatc
     5341 gtggcgtcca gcacgtcctc cgtgtgggcg accgacagga agccggcctc gaaagccgag
     5401 ggtgccaggt agacgccgcc gtcgagcatg gcgtgaaaga accggttgaa agatgccgtg
     5461 tcggaacggg ccacgtccgc gaagttcctg accgggccgt tggtgaagaa aacgctgaac
     5521 atgccgcaca cgtaattgat ggttacgctc acgccgtggc gatttgccga tgcttccagg
     5581 gcttcgcaga aataggccgt gcgctgctcc agttcctcgt agaggggatc gtcgagcgcg
     5641 cgcaaggtcg tcaggcccgc caccattgcc agcgggttgc ccgagagcgt tcccgcctga
     5701 tagatggtgc cttcaggcgc aatgtgcgcc atgacgtcgg cgcgcccgcc gaacgcgccc
     5761 accggcagcc ccccgccgat gatctttccg agtgtggtca gatccggctc cacgccatag
     5821 cgctgctggg cgccgccgcg cgacacgcga aatccggtca tgacctcgtc gaagatgagc
     5881 aggctgccgt ggcggcccgt cagctcgcgg agcccttcca ggtagcctgc ctccggcggt
     5941 acgcatccca tgtttcccgc caccggctcg agaatgaccg cagcgatctg cccagcgtat
     6001 tgttcgaacg cttcggctac tgcgtccaga tcgttgaatg gcagcgtcag cgtctgctcg
     6061 gcgatggccg cgggtacccc gggtgagttc gggatgccca gcgtcagaac gcccgagccc
     6121 gccttgacca gcagggagtc ggaatgcccg tgataacagc cgtcgaactt aacgatgagg
     6181 tcgcgcccgg tgaagcctct ggcgagccgg attgccgaca tggtggcctc ggtgcccgag
     6241 ttcaccatcc gtatcttctc gatgctcggc accatgtcca cgacctgctc cgcgagctcg
     6301 atttcgagct cggtgggcgc gccgaagccc agagctttct cggcctgggc cctcacggcg
     6361 gcgacgactt cggggttgcc atgccccagc aacatcgggc cccaggagcc gacgtagtcg
     6421 atgtaacggt tgccgtccac gtctgtgaga aacgcgccct caccccgctc gaagaaaatg
     6481 ggcgagccgc cgacgccctt gaacgcgcgg acgggggagt tgacgccgcc gggaatggac
     6541 agggccgccc gccgcatcag ttccctcgac ctgtctgtgt gcatcgctcg acccctatcg
     6601 cgtgacggga gctagaaggg tttgaccacc accatgatga ccaccacgat cagcacgagt
     6661 gcgggcagct cattgaacca ccggtagaac gtctcgctgt gggggtcggc atctgccgca
     6721 aaggctctga cgagccgcgc gcagtaatga tgataaccga gcagtgcgac caccgcagtc
     6781 agtttcaccc acagccagcc cgagccggcg aacgcgttcc agccgaggaa gacaagccag
     6841 aggccgaagc cggttgccag cagcatggag ggccgcatga tgacgcggta gagcttgctc
     6901 tccatcacct tgaatcggtc gcggctgatc gcatcggtgc tgctggagtg gtagacgaac
     6961 agcctcggca ggtagaagag ggccgcgaac caggtcacga cgaagatgat gtgaagggcc
     7021 ttcagccaca ggtagccggc gctcatcgtt tggatcaacc tctgtaggtg tcgatgtctt
     7081 cctgggtgac gatgcccagc aggctcccgg ccgccgtcac gcccagccgg cgcacgcaca
     7141 gcgcttcggc agggcttccc tgcagggcca tctgggcttc gtcaacggtg cagcgcacgt
     7201 cgacgctggt cgcgtcccta cgcatgccgg gaatattgag caggtcgacg tcggcggtgt
     7261 caatctgctc ggattctagg tgggcggcca ggtcgctcga cttgaggacc cagcgggtct
     7321 cgccgtcgtc cgatgcgatc gcaatccata acggcctttg ctcgagtgcc cttcgcgcgg
     7381 cgtcgagaga gcagtcacgg ggaagcaccg ccacttcttc gcacatgatg gccgccacgc
     7441 cggcgcgctg catgtgcgcc gtgaccgggc ttggcgggta ctcgagcccc agggtcttca
     7501 gggtggagat gaacaccgat cgctgcctga acgccacgct gctcaccagc gtcgccacca
     7561 cgatcatcag catggcgggc agtattagat tcgggttcag ggtgagctcc atgacggcca
     7621 tgagcgcggc gagcggagcc tgaagcacgg cggccatcat ggccgccatg ccgagcatga
     7681 cgtagaaccc ctcggagatc gcatcggccg gctcgagcag gttgccgatc atgccgatgc
     7741 agccgccgag agccgcgccg atgaccaacg tgggaccgat gaggccaacg ggcaggccca
     7801 ggcccaccgc cgccgcgctg ctgatcgact tgaacagagc gacgagcagc agcgccgtaa
     7861 ggctcagctg tcccagcatt gccgcgttga ccgtgtcgta gccgattccc atcaccgccg
     7921 gcaccatggt agcggcaagc ccggtcaggg ctccggcaag cagggctcgg ctccagaagg
     7981 gccagcggga gagacgcgcg aaaagctgaa ctagcgcggt gaatcctgcc gccgtgcagc
     8041 cgatgacgat cccggccagc acgacgaagg gcacttccga cagggactgc atctgcagtg
     8101 gagccacagt gaatgcgggc gcgtcgccaa tgaaatagtg ggtcagcagg gtggaagtga
     8161 cggcggcgat gatgaccggg atgaagctgc cgatggtgta ctccatcatg acgacctcca
     8221 tcgcgaagat gacccccgcg atgggcgtat taaaggagct ggcgatggcg gccgcgctgc
     8281 cgcaggcgac cagggtgcgc aggctgttgt tgggtagccg gaagatctgg ccgatcaggc
     8341 tcgagcttgc ggcgccaaga tgaatcgcgg gtccttccct tcccccagac tgaccggacg
     8401 ccagggccgc gatgccgccg aaaaactgca ccgcggcgtt gcgccacggc aggtagccct
     8461 ggtgccggga gagccgctcc atgacgtgga cgacgcccac ccgccgctcc gccgcggaca
     8521 ggcggttgag cgccagcccg atcaggccgg cgccggcgat gggcagggcc aggcgctcca
     8581 ggatgctcag cgcttcgaag gattcactgc cttcgggaag cagccagccc ccgaggatga
     8641 gctcgacggc cgcccggaag agcaggatga cgcagccggt gatcagtccg gccagaacgg
     8701 ccagtagcgc cagctgtgga agcgcctcga tgtccgccag ccgattgcgg aagaactccg
     8761 gggagacttt atttggtaat ctcagggccc caatctctgc cgacggtgca gagctcgaac
     8821 tggtgaatcg gatcactagt atagtctctg gaatggcccg aggcagatca tcccgtatgg
     8881 cacgtgaccc attgaaaggc atcatcaaag gcgagggaca ggtgcgcgtg gtggcgcact
     8941 cgccgcgcag gcgcctgctc acgtcattga ccgtcgccct ggtctgcgcg gccggtgctg
     9001 cgggtgggtt ctaccttggc gaatccaagg cggggctgga tgcaggctac atcgttgccc
     9061 tggagaagat ggaccgtgcg aacgcggttg agatcgccga gctccgcagc aggctcatcg
     9121 acgccaatct cgcccgaagc gtggagtcac aggcggcgcg cgagctgcgc gacgacatcc
     9181 ggatcctcag ggacgaggtg gccggtctcc aggaagaggt gactttctat aagagcatca
     9241 tggctccgga cagcatcgag cggggcctgc agattgcgga atttcagctc gttcccggcg
     9301 aagcagtcaa cgaatttacc tatcacctgc tgctgaccca ggtggaggcc cgccgggatt
     9361 ggattcaggg caccgtgacc ctcgatgtgg tgggccgaag aacgccggat ccgtcacgga
     9421 aagatgtaga actggtgctc tccctcacag aaatcgggca aaccgacaca tatcctctga
     9481 gtttccggtt ccggtacttc caggacctca ccgggagcat ggcgttaccc gatggatttc
     9541 tgccggaagc ggtggtggta accgcccgga aacgcggtgc gcgctccgag cagttgcagc
     9601 gaaagtttgc ctgggccatg ggcgaagccg aatgagcgcc gtttcctaaa cggacggcga
     9661 gagcgcgcca gggcccccca ggaacgaagg gtcatcatgg cgagaaccaa ggtccgagac
     9721 ggcgtaaccc tgatcgcagc gaataccgaa gtcaccggga acgtgaagtt caacgggcag
     9781 ctctacgtca gcggtcgggt gaacggcaac attctcgccg ccgaaggctg cgaagccacc
     9841 gtggtggtga gcgaggaggg tgtggtttcc ggcgagatcc gcgttcccaa cgtggtcatc
     9901 aacggcaccg tcgaaggcga catctctgct tccgagcgga tagaacttgc ctgcaaagcc
     9961 agagtcaaag gaagcgtgta ttatgcgctg gtagagatgc agctcggggc ccaggtcgac
    10021 ggccagctgg ttcacgaaca tccccagacg gccgcgtctg aggatgctca taaggccgga
    10081 gcaaagcgca ggaatcccct ggggatcatc aagcaactgc ctaaggcggg cgcgaccgcg
    10141 tcctgaaggg cacccttgac ccggtctggg ggtacttcga taattttccg ccctcgccgc
    10201 ccctcgggcc tcgttcatct tgcggccggg gaaccagtga gagcgtcacc gggtaactca
    10261 atgactggac agctcgacat cgacatcagc ttcaccgagc gcgcggccgc gaaagtgcgg
    10321 gcactcatcg cggatgagga gaatgcggat ctcaaactcc gggtcttcat taccggaggt
    10381 ggatgcagcg gcttttccta cggcttcaca ttcgacgagg aagtagccga agacgatgcc
    10441 gtcgtcgagc gcgaaggcgt tagcatgctc gtcgacagcc tcagctatca gtacctgctg
    10501 ggcgcggagg tcgactacaa ggaaggtctg cagggcgcgc agttcgtggt gcagaatccc
    10561 aacgcgtccg ctacctgcgg atgcggcaac tccttcgcca tatgagaagg atccgggtag
    10621 ccgtcacggc cggtagatac cgccgagaat gcgctctccc gaggcacccg tgacgccggg
    10681 cgcgttgccc ggaaggcttg ccatggtctg gtgagccagc caggcgaaag caccggcttc
    10741 cagagcatcg ccgtccacgc cgtgggcatc ggtcgtctcg atcggggagg gcgtcagctg
    10801 ggccagcctt tccatgagca gccggttgtg gcgtccgccg ccgcagacga gccatctctc
    10861 cgcccgcccc ccccagcgat ccaccgcgtc ggtgatgctg c
//
LOCUS       DBNHLM010000083         2348 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_72157, whole genome shotgun sequence.
ACCESSION   DBNHLM010000083 DBNHLM010000000
VERSION     DBNHLM010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_72157"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..900
                     /locus_tag="AC16QF_04050"
     CDS             28..900
                     /locus_tag="AC16QF_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447755.1"
                     /translation="MMRIVLILSLAFSLVALWVATIRPPVEQRPEPAAGDAGRVASRA
                     PAVTAQPPVDERAEMIRQTAAPDEKNQADPGFDPMLVTGLGDFNAEEIERYNRLHLLP
                     FNPAIGRDCEDRPDPQLPGRSYTSCTTVRERPAHPYSELGDEVLLSLAEHDAAAALML
                     GRRVAGEDERLYWYLRAAALSEKSGPLMALAEQRYRDSMDLRLVRGELRRVPRPDRIA
                     VRVALDTVAGRMGDPRANPDRWRGVLRSLAADRAPTYLAQADALVAEFLGAMARTQRE
                     ITGSVQIQEIIDNA"
     gene            893..1480
                     /locus_tag="AC16QF_04055"
     CDS             893..1480
                     /locus_tag="AC16QF_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447756.1"
                     /translation="MRRRLLMAAALVFPGMAVAEALRTECAGCETLRDFGNYGAAQLY
                     RAVGPMGPAVLNDRIWVVNPDTGRQVFVDLDTPTTLVYFFGAPIPVPDLLRTEINATW
                     GDGSVMETWILPNEVIGSMAEALISVDSAGTPPVNAELLDRLPGFGYGGGWYHAGSVG
                     SMTSTHYGAWSFLVRYDSYGRAVVTVYECAYASVC"
     gene            complement(1500..>2348)
                     /locus_tag="AC16QF_04060"
     CDS             complement(1500..>2348)
                     /locus_tag="AC16QF_04060"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6447757.1"
                     /translation="EVEFISEFARPLPQMVMANVLGFPEEDIPQLAEWGNAQVTPFVY
                     GKGHRNILTDEQIKEQFELLDGFKEYVYDKVQEKRKNPQDDMISFLTQVEYKALGRKL
                     TDMEVNGVVYAMVIGGLETTQYALEEQAQLLCEREGIFDAIRQDREKLRQFTEEAMRL
                     RSPTQGLSTRITSQDEVFQGVKVPKGSMLHLRWAAANIDEQEFECPYELQLDRKAASR
                     HLTFSAGPRVCPGAGISRLEQIIAWNRLLDRIDSLEYAPDNTFLHQPGIMLGTLELHL
                     RFTKAQ"
BASE COUNT          386 a          722 c          771 g          469 t
ORIGIN      
        1 tggacacggt aacgcaagag gtcccgcatg atgcgaatcg tcctgatctt gagtctggct
       61 ttcagtctcg tcgcgctatg ggtggcgacc atccgtccgc ctgtcgagca acgtcctgaa
      121 cccgccgccg gcgatgccgg gcgggtggcg tcccgggcgc cggcggtgac ggcccagcct
      181 cctgtcgatg aacgagcgga aatgattcgg cagacggctg ctcccgatga gaagaaccag
      241 gccgatccgg gtttcgatcc gatgctggtg acgggtctcg gtgacttcaa cgccgaagag
      301 atcgaacggt ataaccgcct tcacctgctc ccattcaatc cagccatcgg gcgtgattgc
      361 gaggatcgtc ccgacccgca gttgcccggg cgctcctaca ccagttgcac aaccgtccgc
      421 gaacgacccg ctcaccctta cagcgagctg ggcgacgagg tgctgcttag cctcgccgag
      481 cacgatgctg ccgcagccct gatgcttggc cgccgcgtcg ccggagaaga cgagcgcctt
      541 tactggtacc tgcgtgctgc agcgctgtca gaaaagagcg ggccgctcat ggccctggcc
      601 gagcagcgtt accgtgactc gatggacctt cggctcgtgc gaggggagtt gcgccgcgtt
      661 ccccgacccg accgcatagc cgttcgcgtt gccctggaca cggtggctgg gcgcatggga
      721 gaccccaggg cgaacccgga ccggtggcga ggggtgctgc gctcccttgc cgccgataga
      781 gcgccaacct atctcgccca ggccgatgcc ctggtggccg agtttctcgg cgccatggcg
      841 cgaacccagc gcgagatcac gggcagcgtg cagatccagg agatcatcga caatgcgtag
      901 acgtctcctg atggccgctg cgcttgtgtt ccctggaatg gctgtcgccg aggccctgcg
      961 caccgaatgc gcgggatgcg aaacgctgcg cgacttcggt aactacggcg cggcgcagct
     1021 gtatcgcgcc gtaggcccca tgggcccggc cgttctgaac gatcggatct gggtggtgaa
     1081 tcctgacact ggcaggcagg tttttgtgga cctggatacg cccactacgc tcgtttactt
     1141 ctttggtgca cccatccccg tgccggatct cctgcgcacg gagatcaacg caacctgggg
     1201 cgacggctcc gtgatggaga cctggatcct gcccaacgag gtgatcggtt ccatggccga
     1261 agcgctgatt tcggtagact ccgcggggac gccgccggtc aatgcggaac tgctggaccg
     1321 cctgccaggc ttcgggtacg ggggagggtg gtatcacgcg ggcagcgtcg ggagcatgac
     1381 gagtactcat tacggcgcgt ggtctttctt ggtgcgctac gacagttatg gacgggccgt
     1441 ggtgaccgtc tacgagtgtg cctacgcgtc cgtttgctga gagagcgcgt cctctttgcc
     1501 tactgggcct tggtgaatct gaggtgcagt tcgagggtgc ccagcatgat gcccggctgg
     1561 tgcaggaacg tgttgtcagg cgcgtactcc aggctgtcga tacgatccag cagacggttc
     1621 caggcgatga tctgctccag ccgggagatg cccgcgccgg ggcagacgcg tgggcctgcc
     1681 gagaaggtga ggtgccggct cgccgccttg cggtccagct gcagctcgta ggggcactcg
     1741 aactcttgct cgtcgatgtt ggcggcagcc caacgaaggt gaagcattga gccttttggt
     1801 accttgacgc cctgaaagac ttcgtcctgg ctggtaatgc gtgtggaaag gccctgggtg
     1861 ggagagcgca ggcgcatggc ttcctcggtg aactgacgca gcttttcccg gtcctgcctg
     1921 atggcgtcga agatgccctc gcgctcgcac agcagctggg cctgctcttc caacgcgtac
     1981 tgggtggtct ccagcccgcc gatcaccatg gcgtaaacga ccccgttgac ctccatgtcg
     2041 gtaagcttgc ggccgagcgc tttgtactcc acctgggtga ggaaggagat catgtcgtcc
     2101 tgggggttct ttcgtttctc ctgaaccttg tcgtagacgt attccttgaa accgtccagc
     2161 agctcgaact gctctttgat ctgctcgtcc gtcaggatgt tgcggtggcc tttcccgtag
     2221 acgaatgggg tgacctgagc gttaccccat tcggccagct gggggatgtc ctcctcggga
     2281 aagccgagca cgttggccat caccatttgg gggagggggc gtgcgaactc cgatatgaac
     2341 tccacctc
//
LOCUS       DBNHLM010000084        10544 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_72260, whole genome shotgun sequence.
ACCESSION   DBNHLM010000084 DBNHLM010000000
VERSION     DBNHLM010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10544)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10544)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10544
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_72260"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="AC16QF_04065"
     CDS             <1..336
                     /locus_tag="AC16QF_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HIR6447758.1"
                     /translation="VDRTTAGYRVSQGGTELDVRVATPTAASLMTHMLEKQAPDLSKF
                     LLSPMPGLLVRLRVAEGEEAKAGEELAVVEAMKMENSLRAEQDVKIAKVLAAEGDSLE
                     VDQPILEFE"
     gene            450..641
                     /locus_tag="AC16QF_04070"
     CDS             450..641
                     /locus_tag="AC16QF_04070"
                     /inference="COORDINATES: protein motif:HMM:NF018058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HIR6447759.1"
                     /translation="MEKFRLGLSAAIAILLIILILQNMVTVEVRLLWWTVAMPRAILL
                     LLTAMAGFAVGILVRIRRR"
     gene            complement(655..3840)
                     /locus_tag="AC16QF_04075"
     CDS             complement(655..3840)
                     /locus_tag="AC16QF_04075"
                     /inference="COORDINATES: protein motif:HMM:NF016260.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HIR6447760.1"
                     /translation="MKRLVAGIVGVLLATVFLVIAAVAVLMLSEPGSRFLVERATRLL
                     PLEVEGVTGSLVGEMRFQRLRYELEGQIVEAQGLEVAFDPMAFLLKDEVRFRYVRAVR
                     LRVETRGGEATSAGLGRFELDAPPITLVIDSLAVQVAEILDLPPSSMTLAGRWGAREL
                     SLAQVAVASEVLELRLSGSLANGREPALDLVGSWSLPGTSWSGSGTLSGPLAALNVRH
                     RLEGPVTLEAQGAVDLSSLPDPRFELEVAMPEFSSEGFLLSDVLATVSGGRERVEGVV
                     EGLVSTPWLTEPVPARLSGSADLPPGEVRIAVDGFQAEVLGRPVEGRLVARQLAAGWR
                     LEDVELDLSGSRVSGSAELDESGARASVHISAPDLASLDLGVAGDASGSVDFAAEAGS
                     LSVSVSVSGSELSSGTARLLDYSVSGESRGREINVVVRGDRAAQAGIEARMPSLQVDG
                     PIDAPAWQLEWQGGAANGIATREGEGLNLRVEALRFVALESSWRLSAPVKASADAAGV
                     RVSAFCIEGPGSSGCVDELEMSGGKLVTAGRLVELPADLVSQWLPLPLVEGAVLEGQW
                     RVVRDDGAWSGSAGLAARRLAYAVAGPGSVPIELPDVTVAAVFDDQVLRYTAEASDTD
                     FELALDGQLSPIAANGALAGTLRLQADDVGPLRAFYQHVRELTGRLEGSVEFGGTLEE
                     PRGAGSISLRDGSLSLERPDLELRELQALLTLTEAGSVEVTGSASQADGRIELRGSGQ
                     DLFAAARAFDVELTGSDLAVEHPDWTVRVSPDLRFTYTAGRGHLAGLIELPQAEIKVE
                     TLPAGAPRVSDDVVVVGRDEQADTRRTRVTVDVTLVLGDQVALNAAGIAAQLEGRLNA
                     RVDAEGRQSLHGALQIEGGAVLTQGQTLTIESGNVVYNGPVDNPYIDLRATRTIDTES
                     PSVTVGVHIRGSADNLTSTIFSDPAMSETRALSYLILGRDIGDPSDTDTSQLAAAAIN
                     LGLRSSSSIVADLQRIAGLDELSAVAETQKSFAIVAGKRIGPSLYVRYTYNTLSTIGA
                     LIVRYDLTRRWRVEALSSEYSAMDLFYRIGR"
     gene            complement(3837..5573)
                     /locus_tag="AC16QF_04080"
     CDS             complement(3837..5573)
                     /locus_tag="AC16QF_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0097347 - TAM protein secretion complex
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HIR6447761.1"
                     /translation="MSVGAFSRSMMRVAAGLLALLVWPAAALGDVSIEGVDEEVRTGI
                     LAFIALDELPCDAPRWWVQRGYRDAVEGSLRALETYGYYRATVQADLVWGEECWTASI
                     DVVPGEPVRLGAVVIEVSLPLADEPDMAALLGNPGLASGERFSHASYETAKGRVLDLA
                     RDLGYLDAAFNRNRVLVDAEAGLADVELALESGLRYRFGPIEIEQDDLRPKLFDRFLT
                     VSEGDDFDGRELRDTQRRLMESDYYDRVLVAPVLDRREDGRVPISVQADASSRRNILL
                     GAGFATDTGPRVRADARYRRVNRKGHRARGGLLGSKVLSEANVEYGLPYGDPTHQWLI
                     LKGSVAYEETETARSFRQTVGVARTHRRGDHWTETNYVEYRFEDFDVGDQEGDSDLLL
                     IGTSWSYTSTVDAPRALSGRTITFDVHGASKALASDTDFLKLVVHGKQIVPLGERFRL
                     IGRISSGWTFKSDFDELPPSERFFAGGDSSVRGYEYESLGPEENDAVVGGTRLLTGSL
                     ELDMLIRPKWSVALFVDSGSAFNDSPDFSTGVGAGLRWYSPVGPIRLDLAHPLDDPDN
                     DFRIHISFGPDL"
     gene            complement(5586..6566)
                     /locus_tag="AC16QF_04085"
     CDS             complement(5586..6566)
                     /locus_tag="AC16QF_04085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HIR6447762.1"
                     /translation="MTVFALIVGIALLLGGGTLLIRGASGVAANAGISPMIVGLTIVA
                     YGTSAPELVVNVVGALENETQIAFGNVIGSNVANLGLILGGTALILPIAIEGRLVRRE
                     LPLLLLGTAVLLVLVLDGPLRAERAVIDRADALVLLLLFSTFIFMTVDQVVRGRQDPL
                     LVGAGQVGAASERGNGGSRNWAEVLLGVAALAGGGHLTIQSGADLAEILGWEKVLVGM
                     VIVAIGTSLPELVTSVIAAVRREPDLCVGNLVGSNIFNGLLVLPISALLRPIDVPAHG
                     VTDVLASFFVAAALIPIFIMGRSRLGRPVGSLLILAYAGYIGYRLATGTA"
     gene            complement(6563..7648)
                     /locus_tag="AC16QF_04090"
     CDS             complement(6563..7648)
                     /locus_tag="AC16QF_04090"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HIR6447763.1"
                     /translation="MGVGKHLNAAFKIAGHTIWPGERVRFDLPGAQLYTHTPLDMPVE
                     VVNGRRAGPTLLICAAIHGDEINGVEIIRRLSALKALSRLHGTLILVPVVNLFGFIHQ
                     SRYLPDRRDLNRCFPGSGGGSLGSRVAHLFFEEVVVRSTHIVDLHTGAIHRDNLPQIR
                     ATLDDPVVREMALGFSIPVIVNAGLIDKSLRSEAGKRGIPVITYEGGEALRFDESCIV
                     AGVRGVTNVMRTLGMLPAKHIRRVRAEPYVSRSTVWVRAPIDGIFRTSVRLGARVGVG
                     DALGSVTSPFATSESTLTSGVGGIIICVNRLPLVNEGEALIHIARFAQAARVEDEIAS
                     HGSEIEEDPLYEPGVLEDVDLGPEPGR"
     gene            complement(7635..8540)
                     /gene="rimK"
                     /locus_tag="AC16QF_04095"
     CDS             complement(7635..8540)
                     /gene="rimK"
                     /locus_tag="AC16QF_04095"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HIR6447764.1"
                     /translation="MKIGILSRNRKLYSTRRLVEAAKARGHEAVVVDTLRCYMNMATR
                     RPSIHYQGKELTGLDAVIPRIGASISFYGAAVVRQFEMMGVYAVNESVAITRARDKLR
                     SMQLLARRDIGMPVTGFAHSPDDIPDLIDMVGGAPLIVKLLEGTQGIGVVLCDNRKSA
                     ESVIEAFLGINVNIMVQEFVQESAGSDIRCFVVGDKVVAAMSRHAQPGEFRSNLHRGG
                     SAKATKITPTERRTAVSAARAMGLNVAGVDLIRSNHGPLVLEVNSSPGLEGIESATGM
                     DVAGRIVEFIEQNGRPGRTRTRGRG"
     gene            complement(8537..8983)
                     /locus_tag="AC16QF_04100"
     CDS             complement(8537..8983)
                     /locus_tag="AC16QF_04100"
                     /inference="COORDINATES: protein motif:HMM:NF017434.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease family protein"
                     /protein_id="HIR6447765.1"
                     /translation="MTKNILSREIIGWREWVSLPGLRIDRVKAKIDTGARTSALHAFE
                     LDTVNRRDGLWVTFAVHPNQRDEESVVRSQAKVTDRRIVRDSGGHEEERFVITTDVEM
                     GGERWPIELTLTARDNMGFRMLLGRTAMRGRYMVDPARSYTTGRRM"
     gene            9118..10080
                     /locus_tag="AC16QF_04105"
     CDS             9118..10080
                     /locus_tag="AC16QF_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HIR6447766.1"
                     /translation="MQTDEDGLVFAMALDGNGGADPVGLSQAGDARPTWLHFDYSSSA
                     TEQSLIHIGLSASITESLTRPDARPRTVAMEGGVLVLLRGVNTNPGANPEDMVSVRIW
                     LEPNRMISVRQRRLLSVQDLKAELEQGRGPRSVPELAVELIERLADRIAIFVDGIEDR
                     AALCEADADEASPTELRATVAELRRQTAAVRRFLAPLRDALESLFRNSKGILDEDRSF
                     RIREQSDRITRYVEDLDLVRERAMVIHEELLNRVAQEQNARVYLLSIVAAIFLPITFI
                     TGLFGMNVAGLPGVDDPRSFWLVAGIMTAVSGGVIVWLRRRRWL"
     gene            complement(10088..10474)
                     /locus_tag="AC16QF_04110"
     CDS             complement(10088..10474)
                     /locus_tag="AC16QF_04110"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6447767.1"
                     /translation="MPNSHLLENTVVNWTLVDRLARTTVRVGVAYGSPTAQVRELISQ
                     AVIEQDGVLEDPKPNVVFEDFGDNSLIFDAYFWANAGGDRDLRTIRSDIRFRIDELFR
                     EHDIVIAFPQRDIHVDGSLVLTRKET"
BASE COUNT         1966 a         3576 c         3261 g         1741 t
ORIGIN      
        1 gtggaccgga cgacggctgg ctatcgggtt tcccaggggg gcacggagct ggacgtccgc
       61 gtggccacgc caaccgcggc aagcctgatg acgcacatgc tcgaaaaaca ggcgcctgat
      121 ctgagcaagt ttctgctctc tcccatgccg ggtctcctgg tccgactcag ggtcgccgag
      181 ggcgaagagg caaaggccgg ggaagagctc gcggtggtcg aagccatgaa gatggaaaac
      241 agcctgcgcg cagagcagga cgtcaagatc gccaaggtgc tcgcggcgga gggggacagc
      301 ctggaagtcg accaaccgat tctcgagttc gagtaggccg gctcacgccc atccgggatt
      361 accgaaaacc gtccacagca gatcgcagca ctgcccaatg ttgaagctcg cccttagaat
      421 gcgccctgac tccaccttcc ggtaagacca tggaaaaatt caggttgggc ttgagtgccg
      481 ccatagcaat tctgctgatc atcctgattc tgcagaacat ggtgaccgtt gaggtccgtc
      541 tgctctggtg gacggtggcc atgccaaggg ccattctgct gttgctgacc gccatggccg
      601 ggttcgcggt cggcattctc gttcggatac gcagaagatg agaagctcac ggctctagcg
      661 gccgatcctg tagaaaagat ccatggcgga gtactcgctg gaaagcgcct ccacgcgcca
      721 gcgcctggtg agatcgtaac ggacgatgag cgcgccgatg gtcgaaaggg tgttgtacgt
      781 atagcggacg tacaggctgg gcccgatccg ttttccggca acgatggcaa agctcttctg
      841 ggtctcggca accgccgaca gctcatcaag gcccgcgatg cgctgcaggt cggcgacgat
      901 ggacgacgag gaccggagcc cgaggttgat cgctgcggcc gcaagctggc tggtatccgt
      961 atccgacggg tcgccgatgt cgcggcccag gatcagatag gaaagcgccc gcgtctcgga
     1021 catcgcgggg tccgagaata tcgttgaagt cagattgtcg gcgctgccgc ggatgtgcac
     1081 gccaaccgtg acagacggac tctcggtgtc gatggtccgg gtagcgcgca ggtcgatgta
     1141 gggattgtcc accgggccgt tatagacgac gttgccggac tcgatggtca gcgtctgacc
     1201 ctgggtaagc acggccccgc cctcgatctg cagggcaccg tgcaacgatt gccgcccctc
     1261 ggcatccacc cgagcgttga gacggccttc cagctgagcg gcgatccccg ctgcgttgag
     1321 cgctacctgg tctcccagga cgagcgtcac gtccaccgtc acgcgggtgc ggcgcgtgtc
     1381 agcctgctcg tcccgaccaa cgaccaccac gtcgtcggaa acacgcggcg cgccggcggg
     1441 aagggtctct accttgatct cggcctgggg cagctcgatg agccccgcaa gatgtccgcg
     1501 gccggccgtg taggtgaacc gcaggtcggg cgacacccgc accgtccagt cgggatgctc
     1561 cacggcgagg tctgaccccg tcagctcgac gtcgaacgcc cgggcggcag cgaagaggtc
     1621 ctgccccgag cctcgcagct cgattcggcc atcggcctgg gaggcgctgc ccgtcacctc
     1681 gacggatccg gcctcggtca gcgtcaacaa cgcttgcagt tccctgagct cgaggtccgg
     1741 acgctccagg ctgagacttc catcgcgcaa agagatcgag cctgcacccc gaggttcttc
     1801 cagcgttccg ccgaactcta ccgagccttc aagtcgcccg gtgagctcgc ggacatgctg
     1861 gtagaaggcc cgcaaaggcc cgacgtcgtc ggcctgcagg cgcagggtgc ccgccaacgc
     1921 gccgtttgcc gcgatgggcg acagctgacc atccaaggcc agctcgaagt ccgtatccga
     1981 ggcctcggcg gtgtaacgca ggacctgatc atcgaagacc gccgcgaccg tcacgtcggg
     2041 cagctcgatg ggcacgctgc ccgggccggc aaccgcatac gccagccgcc gggctgccag
     2101 gccggcactg cccgaccagg ccccatcatc ccggacgacc cgccactggc cctccaggac
     2161 cgcgccttcc accagcggca ggggcagcca ttgagacacg agatccgcag gcagctccac
     2221 caggcggccg gccgtgacga gtttgccgcc gctcatctcg agctcgtcga cgcagccact
     2281 cgagcccggc ccttcgatgc agaaggcaga cacccgcacc ccggccgcgt ccgctgaggc
     2341 cttaaccggc gcgctcagcc gccagctcga ctccagagcg acgaaccgca acgcctcaac
     2401 gcgcagattc agaccctctc cctcacgggt ggcgatcccg ttggctgcgc cgccctgcca
     2461 ctcgagctgc caggctggcg cgtcgatcgg gccgtcgacc tgcaggctcg gcatgcgggc
     2521 ctcgatcccc gcctgcgccg cgcgatcgcc acgaacgacg acgttaatct cgcggccgcg
     2581 actctcccca gacaccgagt agtccagcag cctggccgta ccgctggaga gctcgcttcc
     2641 cgagacactc acggacaccg agagcgaccc cgcctccgcg gcaaaatcga cggagccgga
     2701 cgcgtcaccg gcgaccccga gatccagaga ggccagatcc ggtgcgctga tgtgcaccga
     2761 ggcacgcgcg ccggactcgt cgagctccgc agacccggac acacggctgc cgctcaagtc
     2821 cagctccacg tcctcgagcc gccatcccgc tgccagctgc cgcgccacca gccgaccctc
     2881 cacgggcctg cctagcacct cggcctggaa accgtccacg gcaatgcgaa cctcgccggg
     2941 cggaaggtcc gcactgccgg acaggcgagc aggcacgggc tccgtgagcc acggtgtcga
     3001 gacgagcccc tcgaccacgc cttccacccg ttcgcggccg ccgctcacgg tggccaggac
     3061 gtccgacagc agaaagcctt cactcgaaaa ttcgggcatc gcgacctcga gctcgaaacg
     3121 gggatccggg aggctcgata ggtctaccgc accctgcgct tccagcgtga ccgggccttc
     3181 caggcgatgt cgaacattca gggcggcaag cgggccgctc agcgtgccag agccactcca
     3241 ggatgtgccg ggcagcgacc acgaaccgac caggtcgagc gccggttcgc ggccgtttgc
     3301 cagtgatccg gaaagtcgga gctccagcac ttccgaggcc accgccacct gagcgaggct
     3361 cagctctctg gcgccccagc gcccggccag cgtcatgctc gacggcggca gatccagaat
     3421 ttccgccacc tgcacggcca gcgagtcaat caccagggta atcggcggcg cgtcgagctc
     3481 gaaccggcca aggcctgccg atgtcgcttc tcccccacgg gtttcaacgc gcaagcgcac
     3541 ggcccgcacg tatcggaaac gcacttcgtc tttgagcagg aaggccatcg gatcgaaggc
     3601 gacctcgagt ccctgggcct cgacgatctg cccttcgagc tcgtagcgca gacgctggaa
     3661 gcgcatctcg ccaaccaggc ttcccgtaac gccttctacc tcgagcggaa gcaggcgggt
     3721 tgcgcgctcg accagaaacc ggctccccgg ctcgctcagc atcagcacgg cgacggcggc
     3781 aatcactagg aaaacggttg ccaggagaac gccgacaata cctgcaacaa gacgtttcac
     3841 agatctggcc cgaagctgat gtgaatgcgg aaatcgttgt ccggatcgtc gaggggatgg
     3901 gccaggtcca gccggatcgg gccaaccggc gagtaccagc gcagacccgc gcccacgccc
     3961 gtgctgaaat cgggactgtc gttgaacgcc gagcccgagt cgacgaataa ggcaaccgac
     4021 cacttgggcc ggatcagcat atccagctcc agggagcccg tcagcagccg ggtgccccca
     4081 acgacggcat cgttttcctc aggaccgagg gattcgtact cgtagccccg aacgctgctg
     4141 tcgccgccag caaagaaccg ttcgctgggc ggcagctcgt cgaagtcgct cttgaacgtc
     4201 caccccgacg agatccgccc gatcaggcga aaccgctcgc ccagcggcac gatctgcttg
     4261 ccgtgcacga cgagcttcag gaaatccgtg tccgaggcga gcgccttgct tgcgccatgc
     4321 acgtcgaagg tgatcgtcct gcccgaaaga gcacgtggcg catccacggt gctcgtgtaa
     4381 gaccagctcg tgcctatcag cagcagatcg gaatcgccct cctgatcccc gacgtcgaag
     4441 tcctcgaaac ggtactcgac gtagttcgtt tcggtccagt gatcgccccg ccggtgggtg
     4501 cgcgcgacgc ccacggtctg ccggaagctg cgggccgtct cggtctcctc gtaggcgacg
     4561 ctgcctttga ggatcagcca ctgatgagtc ggatctccat agggcagtcc gtattccacg
     4621 ttggcctcgc tgagcacctt cgagcccagc aggccccctc gagcgcgatg gcccttgcga
     4681 ttgacccggc gatagcgtgc atctgcgcgc actcgcggcc ctgtgtccgt ggcaaagccc
     4741 gcaccgagga ggatgttgcg gcgcgacgag gcatccgcct gcacgctgat gggcacccgt
     4801 ccatcctcgc gccgatccag cacgggggcc accagcaccc gatcgtagta gtcgctttcc
     4861 atgagacggc gttgggtatc ccgcagctcg cgcccgtcga agtcgtcgcc ttcggagacc
     4921 gtcagaaagc gatcgaaaag cttcggacgc agatcgtcct gctcgatctc gatcggtccg
     4981 aatcggtagc gaaggcccga ttcgagggca agctcgacgt ccgccaggcc cgcttccgca
     5041 tcgaccaaca cccgattacg gttgaaggca gcgtcgagat agccgaggtc ccgggccagg
     5101 tccagaacgc gtcccttggc ggtctcgtag gaggcgtggc tgaaacgttc gcccgaggcc
     5161 aggccgggat tgcccagcag cgccgccatg tccggctcgt cggcgagcgg caggctcacc
     5221 tcgatgacca ctgcgccaag gcggaccggc tcaccgggca cgacgtcgat ggacgccgtc
     5281 cagcattctt ctccccagac gagatctgcc tgcaccgtgg cccgatagta cccgtaggtt
     5341 tccagggccc tgagggatcc ctccacggcg tcccgataac ctcgctgaac ccaccagcgg
     5401 ggagcatcgc agggcagctc gtccagggcg atgaaggcaa gaatgcccgt tcggacttcc
     5461 tcgtcgaccc cttcgatgga cacgtcccca agggcggccg cgggccatac gagcaacgcg
     5521 agcaggcccg cggcaacgcg catcatgctg cgggagaatg cacccacgga cattgcggcc
     5581 gcgacctaag ccgttccggt ggcgagtcgg taaccgatgt aacccgcata ggccagtatc
     5641 agcagggacc caacgggtct accgagtcgg cttctcccca tgatgaagat cggaatcagc
     5701 gcggcggcaa caaaaaaaga tgcgagcaca tccgtcacgc cgtgggcagg cacgtcgatt
     5761 ggcctcaaca aggcgctgat cggcagaacc agcagcccgt tgaagatgtt cgatccaacc
     5821 agattgccca cgcagaggtc cggttcgcgc cgcacggccg caatcaccga cgtgacgagc
     5881 tcaggcaggc tggtcccgat cgccacgatg accattccca caagcacctt ttcccagccg
     5941 agtatctccg ccaggtccgc gccgctttga atggtgagat ggccaccacc agccagagcg
     6001 gccacgccga gcagcacttc cgcccagtta cggctcccgc cgttgccccg ctcggaggca
     6061 gcgccgacct ggccggcgcc gaccagcagc gggtcctggc ggccgcgaac gacctgatcg
     6121 acggtcatga agatgaacgt cgagaagagc agaagcaaaa ccagggcatc cgcccgatcg
     6181 atcacagccc tctccgcccg caacggccca tccagcacca gtaccagaag aacggcagta
     6241 cccagcagca gcaacggcag ctcgcggcga accaggcgtc cttcgatggc aatgggcagg
     6301 atcagcgccg ttcctcccag aatcagcccc aggttcgcga cgttggagcc gatgacgttg
     6361 ccgaaggcga tctgcgtctc gttctcgagg gccccgacga cgttgaccac cagttccggc
     6421 gcgctggtcc cataggcgac gatggtgagg ccgacgatca tcggagaaat accggcgttc
     6481 gccgcaacgc ccgatgctcc cctgatgagc agcgtgcccc caccgagcag cagcgcaatg
     6541 ccgacgatca gggcgaagac ggtcaacgac ccggctccgg gccgaggtcg acatcctcga
     6601 gcaccccggg ctcgtagagg ggatcttcct cgatttcgga gccgtgggag gcgatctcgt
     6661 cctccacgcg agcggcttgg gcaaaccgag caatatgaat cagcgcttca ccctcgttca
     6721 cgaggggcag gcggttgacg cagatgatga tgccgcccac tccggaggtc agcgtcgact
     6781 cgctggttgc gaaaggcgaa gtcacggagc ccagcgcgtc gccaacgccg accctcgcgc
     6841 cgagccttac cgaagtgcgg aatatcccgt cgatgggcgc ccgaacccat accgtggaac
     6901 gggatacgta aggctcggcc cgcacccgtc gaatgtgttt tgccggcagc atgcccaggg
     6961 tgcgcatgac gttggtcaca ccccgcacac ctgcaacgat gcagctctca tcgaaacgaa
     7021 gcgcctcgcc tccctcatag gtaatgacgg gaattccacg cttgcccgcc tcgctgcgca
     7081 ggcttttgtc gatgaggccg gcattgacga tcacgggtat ggaaaagccc aacgccattt
     7141 cccgaaccac cggatcatcc agcgtcgccc tgatctgcgg caggttgtcg cggtggatgg
     7201 cgccggtgtg aaggtcgacg atgtgggtgc tgcggacgac cacctcttcg aaaaagagat
     7261 gcgccactct ggagcctagc gagccgccac cactccccgg gaagcagcga ttgagatcgc
     7321 gccggtcggg aaggtagcgg gactggtgaa tgaaaccgaa gagattcacc accgggacca
     7381 gaatcagcgt tccgtgcagc cggctcaatg ccttgagcgc cgaaagccgc cggatgatct
     7441 ccacgccgtt gatctcgtcc ccgtggatgg cagcgcagat cagaagcgtc gggcccgcgc
     7501 gcctgccgtt gacgacctcc acgggcatgt ccagcggggt gtgggtgtaa agctgagcgc
     7561 ccggaagatc gaagcgcacg cgctcgcccg gccagatggt gtgaccggca atcttgaacg
     7621 ccgcgttgag gtgcttaccc acgcccacgc gttctggtcc tgcccggcct gccgttctgc
     7681 tcgatgaact ccacgattcg gccggcgacg tccatccccg tggcgctctc tatgccttcg
     7741 aggccgggcg agctgttcac ttccagcacc agcggcccat ggttggagcg aatcagatcc
     7801 acgcccgcga cgttgagccc catggcgcgc gccgccgaca ccgccgtgcg ccgctcggta
     7861 ggtgtgatct tggtggcttt agccgacccg ccccggtgca ggttagagcg gaactcgcct
     7921 ggctgcgcgt gccggctcat ggcggccacc accttgtcgc caaccacgaa gcagcgaatg
     7981 tccgacccgg cggactcctg gacgaactcc tgcaccatga tgttcacgtt gatgccgagg
     8041 aaagcttcga tgacgctctc cgcggacttt cgattgtcgc agagaaccac cccgatgccc
     8101 tgggtgcctt ccagcagctt gacgatcagc ggcgcgccac caaccatgtc gatgaggtcc
     8161 ggaatgtcgt ccggcgagtg ggcgaatccc gtcaccggca tcccgatgtc tcttcgcgcc
     8221 agcagctgca tggaacgcag cttgtcgcgg gcccgggtga tggccacgga ctcgttgacc
     8281 gcgtagacgc ccatcatctc gaactggcgg acgacggcgg ccccataaaa tgatatggac
     8341 gcaccgatcc tgggaatcac cgcatcgagt ccggtcaact ccttaccctg gtaatgaatg
     8401 gaaggacgtc gcgtggccat gttcatgtag caccgcagcg tgtctacaac gacggcctcg
     8461 tggccgcgag ccttcgccgc ttctacgagc cgccgcgtcg agtacagctt cctgtttctg
     8521 gagagaattc cgattttcac attcgccttc ccgttgtgta cgagcgcgcc ggatcgacca
     8581 tgtaacgtcc tcgcatggcc gtacggccca gcagcatgcg aaaccccatg ttgtctctcg
     8641 cggtcagggt gagctcgatg ggccatcttt cgccacccat ctccacgtcg gtggtaatga
     8701 cgaaacgctc ttcctcatgc ccgcccgagt cacgcactat acgccggtcg gtcaccttgg
     8761 cctgtgaccg gaccacagat tcctcatcgc gctggttggg gtgcacggcg aaagtgaccc
     8821 agagaccgtc acggcgattc acggtgtcca attcgaaggc gtgaagcgct gaggttctgg
     8881 cgcccgtgtc gatcttggcc ttgacgcgat cgatccgaag ccctggcagc gatacccact
     8941 cgcgccagcc gatgatctcc ctgctgagga tgttcttggt gatgaagaac ccccgtcacg
     9001 cggaagccgc gctttctcac gatcgcacca caaggcattg ccttcgggca gtagttctga
     9061 cgagcctttt accctagtct tctcccttta gcgacacttg ccttcattaa ggacatgatg
     9121 cagacggacg aggacggact ggttttcgct atggccctgg atggtaacgg cggcgccgat
     9181 cccgtcgggc tgtcacaggc cggtgatgcg cggcccacct ggcttcactt cgactattcg
     9241 tccagcgcca ccgagcagag cctcatccac atcgggctga gcgcctccat caccgaaagc
     9301 ctgaccaggc cggatgcccg accccgcacc gtggccatgg agggcggagt gctcgtgctg
     9361 ctgcgcggcg taaacacgaa tcccggcgca aaccctgaag acatggtctc cgtgcggatc
     9421 tggctggagc ccaaccggat gatttccgtt cggcagcgcc gtctgctttc tgttcaggat
     9481 ctcaaggcgg agctcgagca gggccgcggt ccccggtcgg ttccggagct ggcagtggag
     9541 ctgatcgagc gcctggcgga ccgcatcgcg atcttcgtcg acggcatcga ggatcgggcc
     9601 gccctctgcg aggcggacgc cgacgaagcc agccccacgg aactgcgggc caccgtcgca
     9661 gaactgcgcc ggcagacggc cgcagtcagg cgctttctgg cacccctgcg cgacgcgttg
     9721 gaatccctgt tccgcaactc caagggcatc ctggacgaag accggtcgtt ccggatccgg
     9781 gagcaatccg atcggataac gcgttatgtc gaggatctcg atctcgtccg cgaacgcgcc
     9841 atggtaattc acgaggaact gctcaaccgc gtcgctcagg aacagaatgc cagggtctat
     9901 ctgctttcca tcgtggccgc gatttttctg cccatcacct tcatcaccgg tctgttcggc
     9961 atgaacgtgg caggactgcc aggcgtcgac gacccccgct cgttctggct ggtcgccggc
    10021 atcatgacgg ccgtatcggg cggcgtcatt gtctggttac gccgacgccg ctggctgtag
    10081 ggccgctcta cgtttctttg cgggtgagca cgagggagcc gtccacgtga atgtccctct
    10141 gcggaaacgc gatgacgatg tcgtgttccc ggaacagctc gtcgatgcgg aaacgaatgt
    10201 cgctgcggat ggttcgcagg tccctgtccc cgcccgcgtt ggcccagaag taggcgtcga
    10261 aaatcaggga gttgtcgcca aaatcctcga aaacgacgtt gggcttcggg tcttcaagca
    10321 ccccgtcctg ctcgatgacc gcctggctga tgagctcgcg gacctgggcc gtcggcgacc
    10381 cgtaggcgac ccccacccgc acggtcgtgc gggcaagccg gtcaaccagg gtccagttca
    10441 ccaccgtatt ctccagcagg tggctgttag gcacgaggag atgcacgccg tctatacggg
    10501 tttaccgatg ctctgcagta caatatcagc tgtttgcggc cagg
//
LOCUS       DBNHLM010000085        11232 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_670645, whole genome shotgun sequence.
ACCESSION   DBNHLM010000085 DBNHLM010000000
VERSION     DBNHLM010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11232
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_670645"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..986
                     /locus_tag="AC16QF_04115"
     CDS             261..986
                     /locus_tag="AC16QF_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447768.1"
                     /translation="MAHSSRMLMMDEAYRLVFRGEFLEGQHPAVARKRLVQALQLNDA
                     QAEKLFSGHPVVVKKRADAATAARYQDLFKQAGARLRVMPLASEAPAAEETTATDAGA
                     SSGASALEVMPSGSDVLRADERSTFEELEIDLSHLAVLDWAPEVPDSAAPEIVAPDYS
                     VADLGVDLAERISREHPGIKADFDLAEPGSLIPNLAVDTAPVVDVAAIEFDVAEVGAD
                     IGDPSDDADVTSPDVSHLSMAVE"
     gene            complement(988..3237)
                     /locus_tag="AC16QF_04120"
     CDS             complement(988..3237)
                     /locus_tag="AC16QF_04120"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HIR6447769.1"
                     /translation="MASSSPAESGSRIPNFFRMSVAERIAALHARGLLSSEDVHRLTA
                     GDHTLRLPVADKMIENVVGVFGLPMGVALNFLVNGRDYVVPLVVEEPSIVAGLSGAAR
                     LARLGGGFRASSTEPILIGQVQVVNVDDPDKAMADLLANRDEILALANSLHPKMVARG
                     GGALDIEVFHHHAPEDGREMVVLHLLVDTRDAMGANLVNTMCEGIASMVETITDGKVF
                     LRILSNLTDRAVATASVRIPAQNLEGKGFSGEDVRDGIILAADLATVDPYRAATHNKG
                     VMNGVDAVAIATGNDWRAIEAAAHAYAARDGQYRSLTRWYKNEDGDLVGEIQIPMKVG
                     TVGGSLETNPSVRINHRLLGTPGATELAGIMAAVGLAQNFAALRALSTDGIQQNHMTL
                     HARSVASTANVPEELFDTVVEALIESGEIKVWKAQEIVKNLTRKIAMPADVERSSACG
                     KVILLGEHAVVYGRPALAVPIPLAVEASVRRGGGDGINLVIPRWGLEQKVRASNAQGL
                     SGILYHVLEQLGLDQENMTIEVIPHVPRAMGLGGSSANAVAILRALDHTFRLRLSDGR
                     LNELAYECETGAHGTASGIDNTIATYGAPLVFERGEDGPRFEEIRVAEPLPLVIGITG
                     KECLTANTVALVRRAWEAHQARYDGIFDQIATLTRAGVDAMRDAHFAELGELMNLCQG
                     YLNALQLSTPELEELIHTARANGALGAKLTGGGGGGSMVALCPDSQDQVARAMEAAGY
                     QALKVTIQP"
     gene            complement(3215..4186)
                     /locus_tag="AC16QF_04125"
     CDS             complement(3215..4186)
                     /locus_tag="AC16QF_04125"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase family protein"
                     /protein_id="HIR6447770.1"
                     /translation="MIHASAPGKVVLWGEYAVLTGAPGLVLAVDRRASCTLTPGGDAW
                     HFETRGLPEPAVDLDVADLTGAHPPGPSGGALAWHVLRHLDSEVPAGATLTTDTASFQ
                     DGGHKLGIGSSAAVCTAVYGAFCELLGIGPSLNDALAVHRSLQGGSGSGIDVAAAYLG
                     GSLRYQLRGGARPPAAEPFDLPDGLILRFIWTGTPAQTTRHVARFEAWRAENQTSTLT
                     ELAQASAALFESGHLVDDLAEYAERLYAMDRAAGLGIYTDAHLQLSQVAKECKVVYKP
                     CGAGGGDIGVAASNNEYRVERFVSAALGLGFQIVEMEKTEHGIQLAS"
     gene            complement(4183..5127)
                     /gene="mvaD"
                     /locus_tag="AC16QF_04130"
     CDS             complement(4183..5127)
                     /gene="mvaD"
                     /locus_tag="AC16QF_04130"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HIR6447771.1"
                     /translation="MGTTARAHPNIALIKYWGKAPGQENIPATPSLSITLDSLVTETH
                     VATGPSRDELYFDDEPVADRKVLDWLEVLRQRYDIPPLEIRTANNFPTAAGLASSASG
                     FAALAVALDAQCGLQLDAATLSDLARRASGSAARSIFGGYVAVQGPRWVAQPVLSPEA
                     WPLKTVIAVTTTARKSVSSSEGMAISAATSPYYPGWVESTRADFTDALAAVRTRDFQA
                     LAELAEFSCLKMHGLMLGSRPGLLYWKAATVSCLHAVRALRQDGVDVFFTVDAGPQVK
                     AICKPSAESDVRAALAAVPGVEQIVVTGLGSGARVIDP"
     gene            complement(5140..6594)
                     /locus_tag="AC16QF_04135"
     CDS             complement(5140..6594)
                     /locus_tag="AC16QF_04135"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6447772.1"
                     /translation="MSDIAFLSATELARRIRDRELSSEELLLHYLQRVDRYNGALNAI
                     VVDIREEALMAARAADAAVARGVDVPPFHGVPMTVKESYNVAGTPTTWGNPKWRGNVA
                     FEDAESVKRLKDAGVIIFGKTNVPLALADFQSFNAIYGTTNNPFDHSRAPGGSSGGSA
                     AALAAGLTGLEAGSDIGGSIRNPAHFCGVFGHKPTWNLLWMRGHAPPGDVRASSDISV
                     IGPLGRSAADLETAVRTMAGPDDIMARGYTLSLPTLKRGGLGGLRIAIWEDDPVCPVS
                     AETKARVLAVASACRDAGAEIIEGARPDFSSEHSHETYQNLLQPTMASRMPDEEYESL
                     LEYVANLDPRDTSRTARVLRAQVARFRDWKRSHELREHLRWKWHLFFQDYDLMLTPMM
                     PTSAFPHDHRPISQRTIEVDGTSMPYMDQIFWAGLTGVAYLPSTVIPTGLDDSGLPIG
                     VQLVGPEYGDLITIGVARELETAGFRFVAPPAYA"
     gene            6673..7695
                     /locus_tag="AC16QF_04140"
     CDS             6673..7695
                     /locus_tag="AC16QF_04140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019471932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6447773.1"
                     /translation="MMNVNRQWVLAKRPYGMVTKDNFEYREETIPEPGDGEVLIRNLY
                     LSFDPTQRGWMEDRESYLPPVQIGEVMRAGSVGQVVESRSDDFSAGDLVQTTGGWQDF
                     TVMSAGMGPMGLRKLPEGVTPRLALGALGVTGLTAYWGMLDLGQPKAGETVLVSGAAG
                     ATGSVAGQIARIKGCRVVGIAGGPEKCAWLKDEARFDDVIDYKNEDVDRRIGELCPNR
                     VDVFFDNVGGGILEAALNHINMRARVVLCGGISAYNATEPQPGPVNLMNLVIMRARME
                     GFIVIDYMDRSHIAVPELLGWINSGEIVHQEDVQEGFENIPDTLNRLFTGRNLGKQLL
                     KIADPS"
     gene            complement(7692..8861)
                     /locus_tag="AC16QF_04145"
     CDS             complement(7692..8861)
                     /locus_tag="AC16QF_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013624.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I family protein"
                     /protein_id="HIR6447774.1"
                     /translation="MYTPFAAATERVPNFAASGLIIRSQGPSAPDPETQRLPRAIASL
                     PGVADRHLNLAKQLERQLARFTDAGQADATVAESARTLWRAAVSDVQNGLDDDRPLYW
                     GRLALRRSVYRSALREKDSLLRSIEHYSRGMGDLRLGDDVLNVLITGFDPFHLETRLD
                     QSNPSGLAALALNGSEFGSGGGRARVCASIFPVRFEDFDDGIVERFFQQILAEREPAM
                     VVTLSMGRDAFDLERFPGRRRSAQDPDNHNVLTGASPDKPLPPGKTGSHLEGPEFLEF
                     SLPVHAMRAADGHWPVRDNRWVTTIERGEFEAGSLADLQSQLAVRGSGGGYLSNEIAY
                     RSRLLHGQLGCSCPIGHVHTPALQGHNAALERLMVGQIRQMLAQAAASLTPRPPS"
     gene            8927..9988
                     /locus_tag="AC16QF_04150"
     CDS             8927..9988
                     /locus_tag="AC16QF_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR6447775.1"
                     /translation="MIFRQLFDADSSTYTYLLGCADTRDAVIIDPVFEQHGRDCALIR
                     ELGLKVRYVLDTHVHADHVTSAWLMHSSLDAEIVLSRRYGIEGVDVAVDHGDVLAFGN
                     CSLTVRATPGHTSGCVTYVTNGQDMAFTGDCLMIRGAGRTDFQAGDVHEMWRSIREQI
                     FTLPDECLIYPGHDYAGRTVSTVGEEKRFNPRIGGDAQEEDFVGYMDNLTLPHPRLID
                     IAVPANMRLGRPENDALPEDVSWGPVVTTFAGVPEIRPDWVARHMAELYVLDVREPGE
                     FEGELGHLEGAHLIPLNQLNERISEVPNDRPVVTVCQSGKRSARAAEILIKAGYDEVA
                     NVSGGLIQWSRLSLPFLEH"
     gene            complement(10156..>11232)
                     /locus_tag="AC16QF_04155"
     CDS             complement(10156..>11232)
                     /locus_tag="AC16QF_04155"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6447776.1"
                     /translation="VIKVEHPSGEVIRVLPPYLPGSTLTVPNETVNRNKENVSLDLHS
                     TEGREIFLKLCERADIVIENFRPGTLASWGVGYEDVAAVKPDIVYVSISGFGQFGLLS
                     HRVGYDPIAQAFSGWASLNGEPEGEPTKAPTFLGDDTAGLHGALAAMAALRHREQTGE
                     GQHVDVSLVDGLLYQSNGYPTAGALGLPLQRWANQFGIAAPANVYECTDGSVFAGVLL
                     DTHWRVLCELLEREDLADLNTMERISRREEVDPILADWCASHTAREVEEIFADLGLPA
                     TRVNTYEDVATNEHVASREMLQTTRLVDGNEIPLTAPPAKFSRTPTRIRSAAPALGQH
                     NASVYAEIGLDEQTLVNLKERGII"
BASE COUNT         1895 a         3562 c         3781 g         1994 t
ORIGIN      
        1 gggtgcgctg gatactcgaa tttcccgatg ttgatgctca tctggagtcc aatgtgcctt
       61 tgcgcgcgcg gcgcccgctt gcgccgttcg agatacctta accagcgcca tgagggacgt
      121 cgagtaacaa acgcacgggt gcccacggct caacgccgat atctgggacg cttttaccaa
      181 tcactggcgc gacttgtgcg cccgtgccca ttttccgctt gcgagaagtg tgaacatgcg
      241 gtcattgcgc cgaagtagat gtggcgcaca gttctagaat gctcatgatg gacgaagctt
      301 accgattggt gttccgcggc gagttcctgg aaggacagca ccccgcggtg gccaggaaac
      361 gcctggtgca ggcgctccag ctgaacgacg cgcaggcgga gaagctcttc agcggtcacc
      421 cggtggtggt caagaagcgc gcggacgcgg ctactgcagc acgctatcag gacctgttca
      481 aacaggccgg cgccagattg cgcgtgatgc cgctggcctc ggaagcaccg gcggccgaag
      541 aaaccacggc gacggatgcg ggcgcaagct ccggtgcgtc cgcgctggag gtcatgccga
      601 gcggcagcga cgttctgcgt gccgacgaac gctccacgtt cgaggagctc gagatcgatc
      661 tcagccacct ggcggtgctc gactgggcgc ccgaggtgcc cgattcggcc gcgccggaaa
      721 tcgtcgcccc ggattactcc gtggcagatc tcggcgtcga cctggcagag cgcatttccc
      781 gtgagcatcc cggcatcaaa gcggatttcg accttgccga gcctggaagc ctgataccga
      841 atcttgcggt ggacaccgcg cccgtggtcg atgtggccgc catcgagttc gacgttgccg
      901 aggtcggtgc ggacatcggc gatccgtcgg acgacgccga cgtcacttcg ccggacgtct
      961 cacacctgag tatggcggtc gagtgaacta cggctgaatc gtcaccttga gcgcctggta
     1021 gccggcagcc tccatggcgc gcgcgacctg atcctggcta tccgggcaca gggcgaccat
     1081 ggacccgccg ccaccacctc ccgtcagctt ggctcccaac gcgccattgg cgcgggccgt
     1141 atggatcagc tcctccagct cgggggtcga aagctgcagg gcgttcagat agccctggca
     1201 aaggttcatc agctcgccga gctcggcgaa atgagcgtcc cgcatggcgt ctacgccggc
     1261 gcgggtcagc gtggcgatct ggtcgaagat cccgtcgtag cgcgcctggt gggcttccca
     1321 cgcccgcctg accagggcga cggtgtttgc ggtgaggcac tcctttccgg tgatgccgat
     1381 gaccagcggc agcggctcgg ccacgcggat ctcttcgaac ctgggaccgt cctcgccccg
     1441 ctcgaagacc agcggcgcgc cgtaggtggc gatggtgttg tcgattccgg aggccgtgcc
     1501 gtgagcgccc gtctcgcatt cgtaggcgag ctcgttgagg cgcccgtcgc tcaacctgag
     1561 ccggaaggtg tggtccagcg cgcgcaggat cgccaccgcg ttggccgatg agccacccag
     1621 ccccatggcg cggggcacgt gaggaatgac ctcgatggtc atgttctcct ggtccagccc
     1681 cagctgctcg agaacgtgat acaagatgcc gctgagtccc tgggcgttgc tggcgcggac
     1741 cttctgctcc agtccccagc gcggtatgac caggttgatg ccgtccccgc cgccgcgacg
     1801 cacggaggcc tctaccgcca gggggattgg caccgccagg gcgggtcttc cgtaaaccac
     1861 ggcgtgctca cccagcagta tcaccttgcc acacgccgag cttcgctcca cgtctgcggg
     1921 catggcgatc ttccgggtca ggttctttac gatttcttga gccttccaga ctttgatctc
     1981 gcctgactcg atgagcgctt cgaccaccgt gtcgaacagc tcctcgggaa cgttggccgt
     2041 gctcgccacg ctgcgggcgt gcagggtcat gtgattctgc tgtatcccat ctgtcgacag
     2101 cgcgcgcaga gcggcaaagt tctgcgcgag ccctactgca gccatgatgc cggcgagctc
     2161 cgtcgccccc ggggtgccca ggagccggtg attgatgcgg acgctggggt tggtttccag
     2221 cgagccgccc acggtcccga ccttcatggg gatttggatt tccccgacga ggtcgccgtc
     2281 ctcgttcttg taccagcgtg tcagcgagcg gtactggcca tcgcgggccg cgtacgcgtg
     2341 agcggcggct tcgatggcgc gccagtcgtt gcctgtggcg atggccacgg catccacccc
     2401 gttcatgaca cccttgttgt gcgtggcggc gcggtacgga tccaccgtgg ccaggtccgc
     2461 ggcgaggatg atgccgtccc ggacgtcttc gccgctgaat cctttcccct ccaggttctg
     2521 cgctggtatg cgcacggagg ccgtggccac ggcgcggtcg gtcaggttgg aaaggatcct
     2581 gaggaaaacc ttgccgtcgg tgatcgtctc gaccatcgag gcgataccct cgcacatggt
     2641 gttgaccagg ttggcgccca tggcgtcgcg ggtgtccacc agcaggtgca gaacgaccat
     2701 ctcgcgcccg tcttcgggcg cgtgatgatg gaatacctcg atgtccaggg cacccccacc
     2761 gcgcgcgacc atcttcggat gcaggctgtt ggccagggcg agaatttcat cccgattggc
     2821 gagcaggtcg gccatggcct tatcggggtc gtcgacgttg accacctgga cctgcccgat
     2881 gaggatcggc tccgtgctgc tggctcggaa cccgccgccg agccgggcca gccgtgcggc
     2941 cccggaaagc ccggcgacga tggaaggctc ttcgacgacc agggggacga cgtagtcgcg
     3001 gccgttgacc agaaagttga gcgcgacgcc catgggtagc ccgaaaacgc cgacgacgtt
     3061 ctcgatcatc ttgtccgcca cgggcagtcg cagggtgtgg tcaccggcgg tcagccgatg
     3121 aacgtcttcc gaactcagga gcccccgggc atgaagcgcg gcgatgcgct cagccacgct
     3181 cattcggaaa aaattgggta tccgcgatcc actttcagct ggcgagctgg atgccatgtt
     3241 cggttttctc catttctacg atctggaagc ccaacccgag ggcggccgaa acgaatcgct
     3301 cgacccgata ctcgttgtta ctggctgcga cgccgatgtc gcccccaccg gcgccgcagg
     3361 gtttgtagac tactttgcat tctttcgcaa cttgcgacag ctgcagatgc gcgtcggtgt
     3421 agatgcccaa tccggcggcg cggtccattg cgtagagtcg ctcggcgtat tcggccaggt
     3481 cgtcgacgag atgcccgctt tcgaacaggg cggcagatgc ctgggcgagc tcggtcaggg
     3541 tgctcgtctg gttttccgcc cgccatgcct cgaaccgcgc gacgtgccgg gtggtttgtg
     3601 ccggggttcc cgtccagatg aagcgcagga tcaacccgtc aggcagatcg aacggctctg
     3661 ccgccggagg ccgggccccg ccgcgcagct ggtagcgcaa cgacccgccg agatacgccg
     3721 cggcgacgtc gatgccgctg ccgcttccac cctgcaggct tcggtgcacg gcgagggcgt
     3781 cgttcaggct cgggccgatt ccgagcagct cacagaatgc cccatagacg gcggtgcaaa
     3841 ccgctgcgct cgacccgatg ccaagcttgt ggccgccgtc ctggaagctg gcggtgtcgg
     3901 tcgtcagggt cgcgccggcc gggacctcgc tgtccagatg ccgcagaacg tgccatgcca
     3961 gcgcgccgcc cgacgggccg ggcgggtgcg ctccggtcag atcggcaacg tcgagatcca
     4021 cggctggctc gggcagtccc cgggtttcga agtgccaggc atcgcccccc ggcgtcagcg
     4081 tgcagctggc ccggcgatcg acggccagca ccagtccggg ggcgccggtg agcaccgcgt
     4141 actcgcccca gagcacgacc ttacccgggg cgctcgcgtg gatcatggat cgatgacccg
     4201 cgcgccggaa cccagacccg tgacgacgat ctgctccaca cccggcaccg ctgccagggc
     4261 cgctctcacg tcgctctcgg cgctcggttt gcagatcgcc ttgacctggg ggcccgcatc
     4321 caccgtaaag aagacgtcca cgccgtcctg tctcagcgcg cgaaccgcgt ggaggcagga
     4381 gacggtggcg gccttccagt acaaaagccc gggccgcgag cccagcatga ggccgtgcat
     4441 tttcaggcag ctgaactccg caagctcagc cagcgcctga aaatcgcgcg ttctgacggc
     4501 ggccagggcg tcggtgaagt cggccctggt gctctccacc catcccgggt agtagggtga
     4561 ggtggcagcg gaaatggcca ttccctcgga ggacgagacg gattttcggg ccgtggtcgt
     4621 caccgcgatg accgtcttca ggggccaggc ttccgggctg agaacgggct gggcgaccca
     4681 gcgcggcccc tgcacggcga cgtagccgcc gaatatcgaa cgcgcggcgg atcccgacgc
     4741 gcggcgggcg agatccgaga gcgtggccgc atccagctgc aggccgcatt gggcatccaa
     4801 agccacggcc agcgcggcga agccggacgc ggacgacgcc agcccggcgg cggttgggaa
     4861 attgttggcc gtgcggattt ccagcggtgg tatgtcgtag cgttgacgca gcacctcgag
     4921 ccagtcgagc accttgcggt ccgccaccgg ctcgtcgtcg aagtagagct cgtcccggga
     4981 tggcccggtg gcaacgtggg tttcggtaac cagggaatcc agggtgatgg acagtgaggg
     5041 cgtggccggg atgttttcct gccccggcgc ttttccccag tacttgatca gcgcaatgtt
     5101 cggatgcgcg cgagccgttg tgcccatggc tgcaacccgt caggcgtaag ccggtggcgc
     5161 aacgaagcgg aagccggcgg tctccagctc cctggccacg ccgatggtga tgaggtcccc
     5221 gtactccggc ccaaccagct gcacgccgat gggcaggccc gagtcgtcca ggccggtcgg
     5281 tatgaccgtg gacggcaggt aggccacccc ggtcagcccg gcccagaaga tctgatccat
     5341 atacggcatg ctggtcccgt cgacttctat ggtgcgctgg ctgatggggc ggtgatcgtg
     5401 gggaaacgcc gaggtcggca tcatcggggt cagcatcaga tcgtagtcct ggaagaacag
     5461 gtgccacttc cagcgcaggt gctcgcgcag ctcgtggctg cgtttccaat cccggaacct
     5521 ggcgacctgg gctctaagca cccgcgccgt ccggctggtg tcccgcggat cgagattggc
     5581 cacatattcc agaagactct cgtactcctc gtccggcatg cgtgatgcca tggtgggctg
     5641 gagaagattc tggtaggtct cgtggctgtg ctctgagctg aagtcgggcc gcgcgccctc
     5701 gatgatctcg gcaccggcgt ccctgcatgc gctcgccacg gcgagcacgc gggccttcgt
     5761 ctcggcgctc accggacaca cggggtcgtc ctcccatatg gcgatcctga gccctccgag
     5821 cccccctctc ttcagggtcg gcaggctcag cgtgtacccc cgcgccatga tgtcgtcggg
     5881 cccggccatg gtacgaaccg cggtttccag gtctgcggca gatcgaccca gcggcccgat
     5941 gacggagatg tctgagctcg ctcgcacatc gcctggcggt gcgtgtccgc gcatccacag
     6001 gaggttccat gtcggcttgt gaccgaagac cccgcagaag tgtgcgggat tgcgaataga
     6061 acctccgatg tcggagccgg cctcgagacc cgtgagcccg gccgccagcg ccgccgccga
     6121 cccccccgac gagccgcccg gcgcgcgcga gtgatcgaat gggttgttgg tggtgccgta
     6181 gatggcgttg aagctctgga agtcggccag cgcgagagga acgttggtct tcccgaagat
     6241 gatcacgccg gcgtccttca gacgcttcac cgattcggcg tcctcgaacg cgacgttgcc
     6301 cctccatttg gggttgcccc aggtcgtggg ggtgccggcg acgttgtatg actccttaac
     6361 cgtcatgggc acaccgtgaa aggggggcac gtcgacgcct cgggctaccg ccgcatcggc
     6421 ggcgcgcgcc gccatcaatg cctcctcccg gatgtccacc acgatggcgt tgagggcgcc
     6481 gttgtagcgg tcgaccctct gcaagtaatg cagcagcagc tcttcgctgg aaagctcgcg
     6541 gtcgcggatc ctgcgggcga gctcggtggc cgagaggaac gcgatgtcgc tcattgtccc
     6601 ctatcagatt cgcagaccgc ggtgactctg acctttgccg atgatttaat caagcgctcg
     6661 catggaggac cgctgatgaa cgtcaacaga caatgggtac tggccaaacg gccgtatgga
     6721 atggtcacca aagacaactt cgagtatcgg gaagaaacca ttccggagcc cggagatggc
     6781 gaagtgctca tccgcaatct ttacctctct ttcgatccga ctcagcgcgg atggatggag
     6841 gatcgtgaaa gctacctgcc gccagtccag atcggcgagg tgatgcgcgc ggggtccgtg
     6901 ggtcaggtcg ttgagagcag aagcgacgac ttcagcgccg gtgatctcgt tcagaccacg
     6961 gggggctggc aggacttcac ggtgatgtcg gcgggcatgg ggcccatggg gctgcgcaag
     7021 ctgccggagg gggtaacacc gcgtcttgcc ctgggcgcgc tcggggtgac cgggctgacc
     7081 gcctattggg gcatgctgga tcttggccag ccgaaggccg gggagacggt gctcgtatcg
     7141 ggggccgcgg gcgcgacggg atcggtagcc ggccagatcg cgcgtatcaa aggatgtcgg
     7201 gtggtcggga tcgccggtgg cccggagaag tgcgcgtggc tcaaggacga ggcgcgcttc
     7261 gacgacgtga tcgattacaa gaacgaagac gtcgaccgcc ggatcggcga gttgtgcccg
     7321 aaccgggtgg acgtcttttt cgacaacgtt ggcggcggaa tcctcgaggc cgcgctgaat
     7381 cacatcaaca tgcgcgcgcg cgtcgtcctc tgcgggggca tatcggcgta caacgccacg
     7441 gagccgcagc cggggccggt gaacctcatg aacctggtca tcatgcgggc gcgcatggag
     7501 ggcttcatcg tcatcgatta catggaccgt tcgcacatcg cggtccccga gctgctcggt
     7561 tggatcaaca gcggcgagat cgttcatcag gaagacgttc aggaaggctt cgagaacatt
     7621 ccggacaccc tgaacaggct gttcaccggt cgtaacctcg gcaagcagct gctgaagatc
     7681 gcggatccga gctaggacgg cggccggggc gtcaggctgg cggcagcctg cgccagcatc
     7741 tgccggatct gtcccaccat gagtcgctcc agcgctgcgt tgtggccctg gagagcgggg
     7801 gtgtggacgt ggccgatggg gcagctgcag ccgagttgcc catggagcag acggcttcgg
     7861 tacgcgatct cgttggacag gtaaccccct ccagaacctc gcacggccag ctgggattgc
     7921 aggtccgcca gcgatcccgc ctcgaactcc ccacgctcga tggtggtgac ccatcggttg
     7981 tcgcggaccg gccagtggcc gtccgccgcc cgcatggcat gcaccgggag agagaactcc
     8041 aggaactcgg ggccctccag gtgcgacccg gttttgcccg ggggcaaagg tttgtccggg
     8101 ctcgcgccgg ttagcacgtt gtgattgtcc ggatcctgcg cggagcgccg ccgtcccggg
     8161 aaacgttcca ggtcgaaggc gtcgcggccc atgctcagcg tcaccaccat ggcgggctcg
     8221 cgctcagcaa ggatctgctg gaaaaagcgc tcgacgatgc cgtcgtcgaa atcttcgaac
     8281 ctgacgggaa atatgctcgc gcagactctg gcccgcccac cgccggaacc gaactcggat
     8341 ccgttcagcg ccagcgctgc caacccggac gggttgctct gatcgagccg ggtctccagg
     8401 tgaaatgggt cgaatcccgt gatcagcacg ttgaggacat catcgccgag ccgaaggtca
     8461 cccatgcctc gggagtagtg ctcgatggac cgaagcagcg agtccttttc acggagggcg
     8521 ctgcggtaga cggatcgccg aagtgccagt cgcccccagt agagcgggcg atcgtcgtcc
     8581 aggccattct ggacatcgct caccgcggcg cgccagagcg tccgggcgga ttcggcgact
     8641 gtcgcatcgg cctgaccggc gtcagtgaag cgcgcgagtt gacgctcgag ctgcttggcg
     8701 agattcaggt gccggtcggc aacgcctggg agcgaggcaa tcgcccgggg cagacgttga
     8761 gtttcggggt ctggagcaga agggccctgg ctacgaatga tgagtccgga cgccgcgaaa
     8821 ttcggcaccc gctccgtggc ggcggcaaag ggagtataca cgtcaggatg cgaggtatcg
     8881 gctcgcgggg tagactttga gcgcaccggt agggtgaggg aacgtcatga tattcagaca
     8941 gcttttcgat gcggactcgt ccacgtatac ctacctgctc gggtgcgcgg atactcgcga
     9001 tgcggtcatc atcgaccccg tcttcgagca gcacggccgc gattgcgcgc tcatcagaga
     9061 gctcggtttg aaagtcaggt acgtgctcga cacccacgtc catgcggatc atgtgaccag
     9121 tgcttggctg atgcacagtt ccctcgatgc cgagattgtg ctctcgcgac gttacgggat
     9181 cgaaggcgtc gacgtcgccg tggaccacgg cgacgtcctt gcattcggca attgctccct
     9241 gacggtgagg gcaacgcccg gtcataccag cggctgtgtg acctacgtga ccaatggcca
     9301 ggacatggcc ttcacgggcg attgcctgat gatccggggc gcaggccgca ccgattttca
     9361 ggcaggtgac gtccacgaga tgtggcgcag catacgcgag cagatcttta ccctgcccga
     9421 tgagtgcctc atctatccgg gacacgatta tgcaggcaga accgtctcca cggtcggcga
     9481 ggaaaagcgc ttcaatccgc ggatcggagg agacgcgcag gaagaggact tcgttggcta
     9541 catggacaac ctgaccctgc cccacccgcg actgatcgac atcgcggtcc cggccaacat
     9601 gcgcctcggc cgcccggaga acgatgcgct gcccgaggac gtgtcctggg ggccggtcgt
     9661 gacgacgttt gccggggtac cggagatccg tcccgactgg gtggcgcggc acatggcgga
     9721 gctgtacgtg ctcgatgtgc gggagcccgg cgagttcgaa ggtgagcttg gccatctgga
     9781 aggcgcgcac ctgatacccc tcaatcagct gaacgaaagg atttccgagg tccccaacga
     9841 ccggccggtg gtaaccgtct gccagtcggg caagaggtcc gcgcgggccg cggagatcct
     9901 cattaaagcg ggatacgacg aggtggcgaa cgtgagcggg ggtctgatcc agtggtcgcg
     9961 gctgtccctg ccgtttctcg aacactagtc acgcgggacg gtctcgctgg ctcgcccatc
    10021 tgggagccgc cctgagccag ggcggggccc gaccaatcaa tcccccctag cgtttaccac
    10081 cgcgccagcg gcgaacgcgg cagtaccgac cgcggctcga ggaactgagg tggttctctg
    10141 cgcgtcggga tcgcgtcaga tgatgcccct ctccttcaga ttcacgagcg tttgctcgtc
    10201 caggccgatc tcggcgtaca cggaggcgtt gtgttggccc agcgccggag cggcgctgcg
    10261 gatccgggtg ggggtgcgtg aaaatttggc cgggggcgcc gtcaggggaa tctcgttgcc
    10321 gtccaccagg cgagtggtct ggagcatctc tctcgaggcg acgtgctcgt tcgtcgccac
    10381 gtcctcgtag gtgttgaccc gagtggcggg aaggcctaga tcggcaaaga tttcctctac
    10441 ttcgcgcgcc gtgtgggacg cgcaccagtc ggccaggatg ggatcgactt cctcacggcg
    10501 tgagatgcgt tccatggtgt tcaagtcggc cagatcctcc cgctccagaa gctcgcagag
    10561 aacgcgccag tgggtgtcga gcagaacgcc tgcaaagacg gagccgtcgg tgcactcgta
    10621 gacgttggct ggcgcggcga tgccgaactg gttggcccac cgctggagcg gaagcccaag
    10681 cgcccccgct gtcgggtagc cgttgctctg gtagagcagg ccatccacga gggagacgtc
    10741 cacgtgctgc ccttctccgg tctgctcccg gtggcgcagc gccgccatgg cggcaagggc
    10801 cccgtgcaga ccggccgtgt cgtcgccgag gaaagtgggc gctttcgtcg gctcgccttc
    10861 cggctcgccg ttgagggacg cccatccgga aaaagcctgt gcgatgggat cgtatccgac
    10921 ccgatggcta aggaggccga actgcccgaa gccgctgatg gagacgtaga cgatgtcggg
    10981 tttgacggcg gccacgtcct catagccgac gccccaggaa gccaaggtcc ctggacggaa
    11041 attctcgatg acaatgtcgg cgcgctcgca tagtttcagg aagatctcgc gtccctcggt
    11101 cgagtgcagg tcgagggaga cgttttcctt gtttctatta accgtctcgt tcggaacggt
    11161 gagcgtggag cctggcaggt acgggggcag aacgcggatc acctctccgg aggggtgctc
    11221 caccttgatc ac
//
LOCUS       DBNHLM010000086         9720 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1509714, whole genome shotgun sequence.
ACCESSION   DBNHLM010000086 DBNHLM010000000
VERSION     DBNHLM010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9720)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9720)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9720
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1509714"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="AC16QF_04160"
     CDS             complement(<1..585)
                     /locus_tag="AC16QF_04160"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6447777.1"
                     /translation="MTVTLSASGLTKVYQTGDVVVHALRGAELELREGELVVMLGPSG
                     SGKSTLLNILGGLDVPTAGTVWFRNRQLTASDEASLTEYRRRHVGFVFQFYNLIPSLT
                     ARENVALVTEISSNPMDAAEALALVGLAERLDHFPAQLSGGEQQRVAIARAIAKRPEI
                     LLCDEPTGALDSETGILVLAAIDQVNRELGTTTAV"
     gene            762..4145
                     /locus_tag="AC16QF_04165"
     CDS             762..4145
                     /locus_tag="AC16QF_04165"
                     /EC_number="6.3.4.14"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase family protein"
                     /protein_id="HIR6447778.1"
                     /translation="MEKLLIANRGEIAVRIARAAAELGLVTVAVYAEDDAASLHRSRA
                     DESEALTGSGPAAYLDIGQMVEVACRSGCDAVHPGYGFLSENPDFAAAVEAAGLTFVG
                     PTPETLALLGDKVSARRLAGTCGVPLLAGSQAPVTLEEARAFLAGLPDGVDMVIKAVA
                     GGGGRGMRVVGSAADLEEAFARCCSEARLAFGDDRVYVEEFLPKARHLEVQVLGDGTG
                     AVSHVWERECSIQRRHQKIVEVAPSPTIGPQLRESLLRAAVDIGEAARYRGLGTIEFV
                     VDGSGERYAFIEANARLQVEHTVTEEVTGLDLVRAQLEVAAGRTLAELSLEQRDIPVP
                     RGYAAQARINMETMLADGTTRPGGGTLTSFEVPTGPGLRTDTFGYVGYTTSPAYDSLL
                     AKLIVHSPAGSFADCARKGYRALCETRIEGVATNLDFLRNLFAQPAFQASEVHTGYVD
                     EHVDELVKEAGHRVYHGGRAAAAPATPVLAGVKVDAADPLAVLDHGKSGGASVAAAPR
                     AIALVDEELPAGVSVIRAPMQGTVVSFEVAPGDEVYVGRQVLVMDAMKMEHVVKAEVS
                     GIVQRLGVNEGDTVFEGHPLVYLQEADIAAGEEAGQEAFDLDYVRPDLAEVFERKAAG
                     HDENRPEAVAKRHGRGHRTARENVADLCDPGSFVEYGSVVVAGQRRRRSMEDLIKNTT
                     GDGMVCGLGRVNGDLFGPERSRAMVMSYDYMVLAGTQGIKNHAKKDRLFEIAEAQRLP
                     TVLFAEGGGGRPGDTDGSGVAGLDCWAFTYFARLSGLVPIVGITNGRCFAGNAVLLGC
                     CDVIIATQGSNIGIGGPAMIEGGGLGIFKPEEVGPMDVQVPNGVVDIAVEDEAQAVAV
                     AKKYLSYFQGPVEDWDAPDERKLRFVVPENRLRYYDMREVIAGIADVGSVLELRQGWG
                     PGIITAFIRVEGMPLGVIANNPGHLSGAIDAEGADKGTRFMQLCDAFDIPILSLCDCP
                     GIMVGPESEKTALVRHAARMFVVGANIDVPLMTIVTRKGYGLGAQAMTGGSFKAPLFT
                     VTWPTGEFGGMGLEGAVKLGYRKELQAIDDPAERKDAYEKMVADMYQRGKAVNMASHF
                     ELDEVIDPAESRRWIVAGLRSVPPAPRRSGKKRPNVDTW"
     gene            4166..4723
                     /locus_tag="AC16QF_04170"
     CDS             4166..4723
                     /locus_tag="AC16QF_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013053.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIR6447779.1"
                     /translation="MQRLDPKSTAVIVIDVQERLAPAMPEAHLAELLRASRILLRGAA
                     LFGSPVLATEQYPRGLGATVPELGEQLGELNVAPREKLSFSACDDEAFMAELEASGAR
                     AAVVVGMESHVCVYQTVRDLVGAGLDVHVPIDGVCSRRDDHRQAGIDLCVRAGAVRTT
                     TESVAFDWLRVAGSPEFKELSGLIR"
     gene            complement(4733..5953)
                     /locus_tag="AC16QF_04175"
     CDS             complement(4733..5953)
                     /locus_tag="AC16QF_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6447780.1"
                     /translation="MRVVVIGAGIGGSALALALDRLGIEHVVLEQAPEFTDVGAGIQL
                     SPNGVRILESLGLRDALKGFCTTPDFHKYSVWDTGETILRTALMPRVREAYGFAYYHA
                     WRPDLIAALTNNLSAGVNMNRKVVRVGQDDGKAWAECEDGERVEGDVLIGADGIHSLV
                     REQVFRPDSPRASGYVTWRGVVESETVADLDIPVSAYVDMGPRLSFVYYYVASGRLLN
                     WLALGQANDDKRESWSQTASKSEVLAAFDGWYERPRRLIEATEQPFVTALHDRNPLES
                     WVSGRIAVMGDAAHAMLPYHAQGAVQSIEDAWVLARTLELHGDDPHGALTRFESLRYQ
                     RANLLVQQSRNAERWYHLDDPVEVAARNERFRRNNDRYGDDFSPQQHWLYSYDAEAAA
                     RGTDEEWRALPTWS"
     gene            complement(5950..6954)
                     /locus_tag="AC16QF_04180"
     CDS             complement(5950..6954)
                     /locus_tag="AC16QF_04180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6447781.1"
                     /translation="MPELRVGVCYDFRNPPDSGVSDARLYAEVMEQVRWLDGIGADLV
                     WFTEHHFVDDGYLPSWTPVAGAMAAVTENVRFGTDICLMPFNHPVRLAEDLAVLDNLS
                     GGRVELGIGMGYAPHEFRGFGLPVSRRVSLMNEGIEVLQRCFTGERFSYHGKRYQLDD
                     VRITPGYVQPGGPPLWIAAMSEGGAARAARYEANFLPQGNRARVYDTWVDALESSGRS
                     PADHRIGIIRSILVTDDRETDWPVIRAAERYRMALYQRFFEESGEGFGDPREAIPQTW
                     IVGNVDQCVAELQQFISDFRITDIVTMAVPPGLRAEQMAPSLERLFTEVVPRLKGGRP
                     "
     gene            complement(7043..7456)
                     /locus_tag="AC16QF_04185"
     CDS             complement(7043..7456)
                     /locus_tag="AC16QF_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HIR6447782.1"
                     /translation="MLDPTTITIFGLVLIKLLLVASIGMHRLYIASTSDKAPNTFLPT
                     GEDVGRFAVRLARAHANCYEFLPFALAVLVYAAATESSALTDGLAYYFLIARIGQSAT
                     HLVSTSTTAVFIRFAFFLAQVVILLVWCLGFLGAL"
     gene            complement(7514..8035)
                     /locus_tag="AC16QF_04190"
     CDS             complement(7514..8035)
                     /locus_tag="AC16QF_04190"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6447783.1"
                     /translation="MALEGEYLGMPVLVDDLDQENLAFFGHCGRGELRLQQCKACGLK
                     RYPPTTACPWCASPDATWEPVAGKGTVYSYGEIHHAIQPAFRQNSPYLLLLVELDEQR
                     DLPAPHDGLRMTGNLATEDGDLAPAELVRSVGIGSRVRVVFKQLGDGIALPLWCLDES
                     AEQPQTPWRYPQE"
     gene            complement(8038..9294)
                     /locus_tag="AC16QF_04195"
     CDS             complement(8038..9294)
                     /locus_tag="AC16QF_04195"
                     /inference="COORDINATES: protein motif:HMM:NF047238.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR6447784.1"
                     /translation="MQADIDATAGRHLRGRYAVVGVGETGYVRGSGCTTRALGTRAVR
                     AALADAGLEPGDVDGMLSYSYNDSTFSPFIAGDLGIRLNFYMDVYGGGSSIEALIGIA
                     MGVIEAGMCRTVVIFRAMNGYSQVRIGGTGGRGAVAPLGGDALFNRAFGQMSAGQMFC
                     QSFMRHMYDYGTTPEQVAMVKVVHSEHASNNPKAYYRQRVSVDDVVNSRMIVKPLHLL
                     DCCVESDTGTAVVVTGIDRARDCRHTPAVIQSVVGRCSKRRSDMHYQSGPISTVAGHY
                     AKDILWPNAGVGPEDIDVTGSYDAFTFTTMLQLEDYGFCKKGEGGDYVSSGITRLGGA
                     RPNNTSGGHLCEGYTHGINMVIENVRQLRHDVDDFCPIGSDGRRVHTYDYTEGGCRQV
                     KDCELTANLGWATPGTGSAMVMARGG"
     gene            9430..>9720
                     /locus_tag="AC16QF_04200"
     CDS             9430..>9720
                     /locus_tag="AC16QF_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6447785.1"
                     /translation="MRLGFSIPNNQGVERVSDLVRLAVEAEELGYASVWVSEHLWHAG
                     YVARRLGEKPYHEPLTVLTAIAAATRQVRLGTSVLVLPWHHPVRLAKTIATLD"
BASE COUNT         1592 a         3174 c         3353 g         1601 t
ORIGIN      
        1 cacggccgtg gtggtgccga gctcacggtt gacctggtcg atggcggcca gcacgaggat
       61 gccggtttca ctgtccaggg ccccggtcgg ctcgtcgcag agcaggatct cggggcgctt
      121 cgcgatcgcc cgagcgatgg ccacacgctg ctgctcgcct ccggagagct gggccgggaa
      181 gtgatccaga cgctcggcga ggccgaccag cgcgagggcc tccgcagcgt ccatggggtt
      241 cgaggagatc tcggtgacca gggcgacgtt ttcccgggcg gtcaggctgg gaatcaggtt
      301 gtagaactgg aagacgaagc ccacgtgccg gcgccggtac tctgtgagag aggcctcgtc
      361 ggaggccgtc agctggcgat tgcggaacca gaccgtcccg gccgtgggca cgtccagacc
      421 gccgaggatg ttcagcagcg tggacttccc cgaccccgaa gggcccagca tcaccaccag
      481 ctcaccttcg cgcagctcga gctcggcccc gcgcagcgcg tgcacgacga cgtccccggt
      541 ctggtagacc ttggtgagac cgcttgcgct caaggtgaca gtcatggttt cgccttcgtc
      601 gggaagccca gattagatga ttctgcgggc agaaccacca tccgcacttt cacgaggttg
      661 cccgttggac ttcgactgat caacgtcaga caatcgccga tcgtttttct gcctttcggg
      721 gagctggccg cccatctcac gggaacggtc gaggagggag cttggagaaa ctgctgattg
      781 ccaatcgggg cgagatagcc gttcgtatcg cccgtgcggc tgccgagctc ggcctggtca
      841 ccgttgccgt ttatgccgag gacgatgctg cctccctgca ccgctcccgg gcggacgaat
      901 ccgaagcgct cacgggcagc ggccccgcgg cctacctgga cattggccag atggtggagg
      961 tggcgtgccg gtcgggatgc gacgccgtcc atcctggcta cggctttttg agcgagaacc
     1021 ccgattttgc cgcagccgtg gaggcggccg gtctcacctt cgtcggcccg actccggaaa
     1081 ccctcgcgct gctcggtgac aaggtgagcg cgcgccggct tgccggcacc tgcggcgtgc
     1141 cgctgctggc gggcagccaa gcgccggtta cgctcgagga ggcccgcgcc ttccttgccg
     1201 gcctcccgga cggcgtggac atggtgatca aggccgtcgc cggcggcggt ggccggggca
     1261 tgcgggtggt cggcagcgct gccgatctgg aagaggcttt cgcgcgctgc tgctcggaag
     1321 ccaggctcgc ctttggtgac gatcgcgtct acgtagagga attccttccc aaagcccgcc
     1381 acctggaggt gcaggtgctg ggcgacggca ccggcgccgt gagccacgtc tgggagcgcg
     1441 aatgcagcat acagcggcgc catcagaaga tcgtggaggt ggcgcccagc ccgacgatcg
     1501 gtcctcagct gcgcgagtcg ctgctgcgcg ccgccgtgga catcggggag gccgcacgtt
     1561 atcggggcct cggcaccatc gagttcgtcg ttgacggttc cggtgagcgc tacgccttca
     1621 ttgaagccaa cgcgcggctg caggtcgagc acacggtcac cgaggaagtg accgggttgg
     1681 acctcgttcg cgcccagctg gaagttgccg ccggccgcac cctggccgag ctctccctcg
     1741 agcagcgcga catcccagtg cccaggggct acgcggccca ggcgcggatc aacatggaga
     1801 ccatgctagc ggacggcacc accaggcccg gtggcggcac gctgacgagc ttcgaggtgc
     1861 ctaccggccc cggtctgcgt acggacacct tcggttatgt cggctacacc acgagcccgg
     1921 cctatgactc gctcctggcc aagctcatcg ttcactcccc cgcaggcagc tttgccgact
     1981 gtgcccgcaa aggctatcgg gccctctgcg agacccgcat cgagggggtg gcgaccaatc
     2041 tggacttcct ccgcaatctc ttcgcccagc ccgccttcca ggccagcgag gtgcacacgg
     2101 gctacgtgga cgagcacgtg gacgagcttg tcaaagaagc gggccacagg gtctatcatg
     2161 gaggacgcgc tgccgctgcg ccggccacgc ccgtgctggc cggcgtaaag gtggacgccg
     2221 cggatccgct cgccgttctc gatcacggta agtctggcgg ggcgagcgtc gcagcggccc
     2281 cgcgggccat cgcgctggtg gacgaagagc tgcccgcggg ggtttcggtc atccgcgcgc
     2341 ccatgcaggg aaccgtcgtc agcttcgagg tggcacccgg cgatgaggtc tacgtcggca
     2401 ggcaggtgtt ggtcatggac gccatgaaga tggagcacgt ggtcaaggcg gaagtcagcg
     2461 gcatcgtcca gcgcctgggc gtgaatgaag gcgacacggt cttcgagggc caccccctcg
     2521 tctatctgca ggaagcagac atcgccgcgg gcgaggaggc cggccaggaa gcgttcgatc
     2581 tggactacgt tcgcccggac ctcgcggagg tgttcgaacg gaaggcggcc gggcacgacg
     2641 agaaccgccc ggaggctgtg gccaaacgcc acggcagggg acaccgaacg gcgcgggaga
     2701 acgtcgccga tctgtgcgat ccgggttcgt tcgtggagta cggatccgtg gtggtcgccg
     2761 gacagcgtcg ccgccggagc atggaggacc tgatcaaaaa caccacgggc gacgggatgg
     2821 tctgcgggct cggccgggtc aacggcgacc tgttcggccc tgaacggtcc cgcgccatgg
     2881 tgatgtccta cgactacatg gtgcttgccg ggacccaggg catcaagaat cacgcgaaga
     2941 aagaccgtct gttcgagatc gccgaagccc agcgcctgcc caccgtgctg ttcgccgaag
     3001 gcggtggtgg ccgcccgggc gacaccgacg ggtccggcgt tgcgggcctg gattgctggg
     3061 ccttcactta tttcgcccgc ctctcgggcc tggtgcccat cgtcggcatc accaacgggc
     3121 gctgcttcgc cggcaacgcc gtgcttctag gctgttgcga cgtgatcatc gccacgcagg
     3181 gttcgaacat cggcatcggc gggccggcga tgatcgaggg gggcgggctc ggcattttca
     3241 agcccgaaga ggtcgggccc atggacgtgc aggtgcccaa cggggtggtc gacatcgcgg
     3301 tggaggacga ggcacaagcc gtggcggttg ccaagaagta cctgtcgtac tttcagggac
     3361 cggttgaaga ctgggacgcg ccggacgaga gaaagcttcg attcgtcgtc cccgagaacc
     3421 ggctgcgcta ctacgacatg cgcgaggtga tagccggcat cgccgacgtc ggctcggtgc
     3481 tggagctgcg ccagggttgg gggcccggca tcatcaccgc gttcattcgc gtggagggga
     3541 tgccgctcgg cgtgattgcc aacaatcccg gacatctgtc cggcgccatc gacgccgagg
     3601 gcgcggacaa gggcacccgc ttcatgcagc tgtgcgacgc cttcgacatc cccatcctga
     3661 gcctgtgcga ttgcccgggc atcatggtgg ggccggagag cgagaagacg gccctcgtcc
     3721 gccatgcggc ccgcatgttc gtggtgggcg cgaacatcga cgttccgctg atgaccatcg
     3781 tcacccgcaa ggggtacggg ctcggtgctc aggccatgac cggcggcagc ttcaaggcgc
     3841 ccctgttcac ggtgacctgg cccacgggcg agttcggcgg catgggtctc gaaggggccg
     3901 tcaagctcgg ataccgcaaa gagctgcagg ccatcgacga cccggccgag cgcaaagacg
     3961 cctacgagaa aatggtggcc gacatgtacc agcgcggcaa ggcggtgaac atggcctcgc
     4021 acttcgagct cgacgaggtc atcgacccgg cggaatctcg caggtggatc gtcgcaggac
     4081 ttcgctcggt gcctccggca ccgcggcggt ccgggaagaa acggccgaac gtagacacct
     4141 ggtagcccaa gcaaggggag agcatatgca gcgtctggac ccgaaatcca ctgccgtcat
     4201 cgtcatcgac gttcaggagc gcctcgcgcc cgccatgccg gaggcgcacc tggcggaact
     4261 cctgcgcgcc agccgcatcc tgcttcgcgg cgcggcgctg ttcggctctc ccgtgctggc
     4321 caccgagcag tatcctcgcg ggctgggggc caccgtcccg gagcttggcg agcagctcgg
     4381 cgagctcaac gtggcgccgc gcgagaagct cagcttcagc gcctgcgacg acgaggcttt
     4441 catggcggag ctcgaagcca gcggcgctcg cgccgcggtg gtggtcggca tggaatcgca
     4501 cgtctgcgtc taccagaccg ttcgagacct tgttggcgcc ggtctcgacg ttcacgtgcc
     4561 catagacggc gtgtgctcgc gccgcgacga tcatcggcag gcggggatcg atctgtgcgt
     4621 gcgcgcggga gccgttagaa cgaccaccga gtcggtcgcc ttcgactggc tgcgcgtggc
     4681 gggaagccca gagttcaagg agctgtccgg gctgattcgg taggcggccc ggtcaggacc
     4741 aagtcggcag cgcgcgccac tcctcgtcgg ttccgcgcgc agcggcctcg gcatcgtagc
     4801 tgtacaacca gtgctgttgc ggtgagaagt cgtcaccgta gcgatcgttg ttccggcgga
     4861 aacgctcgtt gcgtgcggcc acctcgacgg gatcgtccag gtgataccag cgctcggcgt
     4921 tgcgcgactg ctgcaccagc aggttggcgc gctggtagcg cagcgattcg aaccgcgtaa
     4981 gcgccccgtg ggggtcgtcg ccgtgaagct cgagggttcg ggccagcacc caggcatcct
     5041 cgatggactg caccgcgccc tgggcatggt aggggagcat ggcgtgggcg gcatcgccca
     5101 tgacggcgat gcgcccggac acccagctct ccagcgggtt gcggtcgtgc agcgcggtaa
     5161 cgaaaggctg ctcggtagcc tctatcaggc gcctgggccg ctcgtaccag ccgtcgaaag
     5221 cggccagcac ctcggatttg ctggcggtct gagaccagga ctcgcgcttg tcgtcgttgg
     5281 cctgtcccag ggcgagccag ttcagcagcc ggccagaggc gacgtagtag tagacgaagg
     5341 acagccgcgg tcccatgtcc acgtaggcgg acacgggaat gtccagatcg gccaccgtct
     5401 cggactcgac gaccccgcgc caggtcacgt atccggacgc tctcggcgag tccggccgga
     5461 agacctgctc ccggacgagg gagtgaatgc cgtctgcgcc gatgagcacg tcgccctcga
     5521 cgcgctcgcc gtcctcgcac tccgcccagg ccttgccgtc gtcctgaccc acgcggacga
     5581 ccttccggtt catgttcacc ccggccgaaa ggttgttggt cagcgcggcg atgaggtccg
     5641 ggcgccaggc gtgatagtag gcaaagccgt acgcttcccg tacccggggc atcagcgccg
     5701 tccgcagaat ggtctcgccg gtgtcccaga cgctgtactt gtggaaatcc ggagtcgtgc
     5761 agaagccctt gagcgcatcg cgcaggccga ggctttccag gatccgaacg ccgttggggc
     5821 tgagctggat gcccgcgccc acgtcggtga actcgggcgc ctgctcgaga accacgtgct
     5881 cgataccgag ccgatccagt gccagcgcca gcgcggagcc gccgatgccc gcgccgatga
     5941 cgacgaccct catgggcgac ctcccttcaa acgaggaacc acctcggtga acagacgctc
     6001 caggctcggc gccatctgct cggcgcgtag cccgggcggg actgccatgg taacgatgtc
     6061 ggtgatccgg aaatcgctga tgaactgctg cagctcggcg acgcactgat ccacgttgcc
     6121 gacgatccag gtctggggga tggcttcccg cgggtcgccg aaaccctcgc cgctttcctc
     6181 gaagaaccgc tggtacaggg ccatgcgata gcgttcggcg gcacggatca cgggccagtc
     6241 cgtttccctg tcgtcggtca cgagaatgct gcggatgatg ccgatccgat gatcggccgg
     6301 gctgcggccg gagctttcca gagcatcgac ccacgtgtcg tagacgcgcg ccctgttccc
     6361 ctggggcagg aaattggctt cgtaccgggc cgcccgcgcc gcacctcctt cggacatggc
     6421 ggcgatccac aacggcgggc cgccgggctg cacgtacccc ggcgtgatac ggacgtcgtc
     6481 gagctggtag cgcttgccgt gatagctgaa acgctcgccg gtgaagcagc gttgcagcac
     6541 ctctatgccc tcgttcatca gggagacccg gcgtgatacc ggcaggccga agccgcggaa
     6601 ctcgtgggga gcgtagccca tgcctatgcc gagctcgacc cgcccccccg acaggttgtc
     6661 gagcacggcc agatcctcgg ccaggcggac gggatggttg aagggcatca ggcagatgtc
     6721 ggtgccgaag cgcacgttct ccgtgactgc agccatggcg ccggccaccg gcgtccagct
     6781 gggcaggtag ccgtcgtcga cgaagtggtg ctcggtgaac cagaccaggt cggcgccgat
     6841 accgtccagc cagcgaacct gctccatgac ctcggcgtac agccgcgcgt cgctcactcc
     6901 ggaatcaggc ggattgcgga agtcgtagca gacgccgact cgcagttcag gcatgggaat
     6961 atccggctgg ggcatgggcc cgagcatagc atgcgtgcca tgccctcaac gctgcgcccg
     7021 gccggcgacg ccttcgccga gctcagagcg cgccgagaaa gcccaggcac cagacgagca
     7081 gaatcaccac ctgcgccagg aaaaacgcga agcggatgaa cacggccgtc gtgctcgtcg
     7141 aaaccaggtg cgtggccgat tgtccgatgc gtgcgatcag aaagtagtag gcgagcccgt
     7201 cggtgagcgc gctgctttcc gtggccgccg cgtagaccag gacggccaga gcgaagggca
     7261 gaaactcgta gcagttggcg tgggcccggg ccagccggac ggcaaaccgg ccgacgtcct
     7321 cgccggtggg cagaaaggtg ttgggcgcct tgtcagacgt cgatgcgatg tacaggcggt
     7381 gcatgccgat gctggcgacg agcagcagct tgatcagaac cagaccgaag atggtgatgg
     7441 tggtggggtc gagcattgaa ttctctcctt gccttgttgt ttcgtgtggg aggcgtcggg
     7501 cagatgtcgg cgatcactcc tgggggtaac gccagggggt ctgtggctgc tcggcggact
     7561 cgtccaggca ccaaagcggc agcgcgatgc cgtcgcccag ctgtttgaac actacccgca
     7621 cgcggctgcc gatacctacg ctgcgaacga gctcggcggg cgccaggtcg ccgtcctcgg
     7681 ttgccaggtt gcccgtcatg cgcagcccgt cgtgtggggc cggcaggtcg cgctgctcgt
     7741 cgagctccac caggagcagc aggtacgggg agttctggcg gaatgcgggc tggatggcgt
     7801 ggtggatctc gccgtaggag tacacggtgc ccttgccggc caccggttcc caggtggcgt
     7861 ccgggctcgc gcaccacggg cacgccgtgg tgggtgggta acgcttcagc ccgcaggcct
     7921 tgcactgctg caggcgcagc tcgccccggc cgcaatggcc gaagaacgcc aggttttcct
     7981 ggtccaggtc gtcgaccagc acgggcatgc cgagatactc gccttcgagc gccattctca
     8041 gcctcctctc gccatgacca tggccgagcc ggtaccgggc gtcgcccagc cgaggttggc
     8101 cgtcagctcg cagtccttca cctggcggca gccgccctcg gtgtagtcgt aggtgtgcac
     8161 gcgacgcccg tcggaaccga tcgggcagaa gtcgtcaacg tcgtggcgca gctgccggac
     8221 gttctcgatc accatgttga tgccgtgggt atagccctcg cacaggtggc cgccgctcgt
     8281 gttgttcggc cgggccccac ccaggcgcgt gatcccggag ctcacgtagt cgccgccctc
     8341 gcccttcttg cagaagccgt agtcctcgag ctgcagcatg gtggtgaagg tgaaagcatc
     8401 gtaggaaccg gtgacgtcga tgtcctctgg gcccacgccc gcgttgggcc agaggatgtc
     8461 cttcgcgtag tggccggcca cggtggaaat cggccccgac tggtagtgca tgtcggaccg
     8521 cctcttgctg cagcgcccca ccaccgactg gatcaccgcc ggcgtgtgcc ggcagtccct
     8581 ggcgcggtcg atgccggtga ccacgacggc cgtgccggtg tccgactcca cgcagcagtc
     8641 cagcaggtgc aggggcttga cgatcatgcg gctgttgacg acgtcgtcca cgctcacccg
     8701 ctgcctgtag taggccttgg ggttgttgga ggcatgctcc gagtgaacga ccttgaccat
     8761 ggcgacctgc tccggcgtgg tgccgtagtc gtacatgtgg cgcatgaacg actggcagaa
     8821 catctgcccc gcgctcatct ggccgaacgc ccggttgaac agcgcgtcac cgccgagtgg
     8881 cgccaccgcg ccgcggccgc cggtgccacc gatacgtacc tggctgtacc cgttcatggc
     8941 ccggaagatg accaccgtgc gacacatgcc cgcctcgatg acgcccatgg cgatcccgat
     9001 cagcgcctcg atgctcgagc cgccgccgta gacgtccatg taaaagttca gacggatccc
     9061 caggtcgccg gcaatgaagg gagagaaggt cgagtcgttg tagctgtagg acagcatgcc
     9121 gtcgacgtcg cctggctcaa gccctgcatc ggcgagggcg gcccggacgg cgcgggtgcc
     9181 cagggcgcgg gtcgtgcagc cggacccgcg aacgtaaccg gtctcgccca cgcccaccac
     9241 ggcgtacctg ccgcgcaggt gccgtccggc ggttgcgtcg atgtcagcct gcatgggctg
     9301 ctcctctccc aaagctgcct gccgtttctt ttagcactaa tcgaggtgcg ggagtaccgg
     9361 cgatcgtgaa atactcgccg aaccaatccg ggcaggaccg gaggcagggc tgggggcccg
     9421 tttcgagcta tgcggcttgg attcagcatt ccgaacaacc agggcgtgga gcgcgtctcc
     9481 gacctggtgc ggctggcggt tgaggcggag gagctcggtt acgcgtcggt gtgggtgagc
     9541 gagcatctct ggcacgcagg ctacgtggcc aggcgcctgg gcgagaagcc ctatcatgaa
     9601 cccctgacgg tgctcaccgc cattgccgcc gccacccggc aggtccgcct gggcacgtcc
     9661 gtgctcgtgc tgccctggca tcatccggtt cggctggcga agaccatcgc cacgctggac
//
LOCUS       DBNHLM010000087         2200 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1149625, whole genome shotgun sequence.
ACCESSION   DBNHLM010000087 DBNHLM010000000
VERSION     DBNHLM010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1149625"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="AC16QF_04205"
     CDS             complement(<1..998)
                     /locus_tag="AC16QF_04205"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR6447786.1"
                     /translation="MLRRVLATVFLCTALPLCHAAMTAADLGRLLFFDANLSLQRTQS
                     CASCHDESRAFSDGRDNGVRGAASLGDDGRSLGDRNAPALTYVALTPPFRIRGEGQPA
                     GGFFLDGRVATLQEQAAEPFVNPLEMQMPDPTAVVARVRENPTYERSLRRLFGEAVFE
                     DTDTAYRAVGESLAAFEQADELASFDSRYDRHLRGEYQMSEQEALGRDLFFSQLTNCS
                     LCHALSPHATNDARETFTDYGFRNIGVPANAHLRSANGTKPGHTDRGLLENPAVERAE
                     HAGKFKVPTLRNVAVTAPYMHNGVFRELRTAIHFYNRYLVRNPASMTNPETGEPWAP"
     gene            complement(1028..>2200)
                     /locus_tag="AC16QF_04210"
     CDS             complement(1028..>2200)
                     /locus_tag="AC16QF_04210"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006720 - isoprenoid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6447787.1"
                     /translation="WVRTVKWTREHEAGGALFSAFNPMDSDESVRGMETDGLANTNVV
                     WHGGKLLALEEAHAPFELDPYTLESKGAWRYDGKLEGPMTAHPKIDCETGEMLMFGYN
                     AKGLISEDMAFHVVDGDGNLTRSEWFKAPYASMVHDFMITRDHVIFPIMPLTGSMERA
                     MQGGPVYAWEPDKGVHIGIMPRNGSVDELRWFKGDPSFVFHPMNAHSDGNMVTCDVCE
                     FEEAPLFPAPDGTPGDPDKAVPRLTRWTFDLGSNTDDYSSERLDDLACEFPRLDERFT
                     GLGYRHGYFACDLKPEFQVGAFNGIGHVDHATGKIDVYAVGQACATNEPIFVPRTEDS
                     AEGDGFLLANVYDAERKASHLVILDAQNVSAGPLAKAYLDHRVPFGFHGNWMPGQA"
BASE COUNT          334 a          698 c          770 g          398 t
ORIGIN      
        1 ggcgcccacg gttcgccggt ctccggattg gtcatgctcg cggggttgcg caccagatag
       61 cggttgtaga aatgaatggc cgtgcggagc tcgcgaaaca ccccgttgtg catgtacggg
      121 gccgtcaccg ccacattgcg tagagtgggc accttgaact tgccggcgtg ctccgcgcgt
      181 tcgacggccg ggttttcgag caggccgcgg tccgtgtgtc cgggtttcgt gccgttcgcg
      241 ctgcgcaggt gggcgttggc cggcacgccg atgttgcgga acccgtagtc cgtgaacgtc
      301 tcccgcgcgt cgttggtggc gtgggggctg agcgcgtggc agaggctgca gttggttagc
      361 tgagagaaga acagatcccg gccgagggcc tcctgctcgc tcatctgata ctcgccccgc
      421 agatgccggt cgtagcggga atcgaagctg gcgagctcgt ccgcctgctc gaaggcggcg
      481 aggctttcgc caacggcgcg gtaggccgtg tcggtgtcct cgaataccgc ctctccgaac
      541 agccgccgca aggaacgctc gtaggtcggg ttttcccgca cccgcgccac cacggcggtg
      601 ggatcgggca tctgcatctc cagcgggttc acgaacggct ccgcggcctg ttcctgcagg
      661 gtggcgacgc gcccgtcgag gaagaagccg cccgctggtt ggccctcgcc gcggatccgg
      721 aacggcggcg tcaaggcgac gtaggtcagt gctggggcgt tccggtcgcc caaagaccgc
      781 ccgtcgtctc ccagggaggc cgcgcctctc acgccgttgt cgcgaccgtc ggaaaacgcc
      841 cgggactcgt catggcagct ggcgcaggac tgggtccgct gcagtgagag gttggcgtcg
      901 aagaacagca gtcggccgag atcggcggcc gtcatcgccg cgtggcagag cggcaacgct
      961 gtgcacagga aaaccgtcgc cagcacgcgc ctgagcatcg agccttccgg gtcgggccga
     1021 gccgtgctca ggcctggccc ggcatccagt tgccgtggaa gccgaagggt acccggtgat
     1081 ccaggtaggc cttggccagc ggcccggcgc tcacgttctg ggcatcgaga atcacgaggt
     1141 ggctggcttt gcgctcagcg tcgtagacgt tggcgagcag aaagccgtca ccctccgccg
     1201 agtcctcggt gcggggcacg aagatcggct cgttggtcgc gcaggcctgc cccacggcgt
     1261 agacgtcgat cttgcccgtg gcgtggtcca catggccgat accgttgaac gcgccaacct
     1321 ggaactcggg cttcaggtcg caggcgaagt agccgtgccg gtagccgagc ccggtgaagc
     1381 gctcatccag tcgcgggaac tcgcaggcga gatcgtcgag ccgctcgctt gagtagtcgt
     1441 cggtgttgct gcccaggtcg aacgtccatc gggtgagccg ggggactgcc ttgtcggggt
     1501 cgcccggggt gccgtcgggt gccgggaaga gaggggcctc ctcgaactcg cagacgtcgc
     1561 aggtcaccat gttgccgtcg ctgtgcgcgt tcatggggtg aaagacgaac gagggatccc
     1621 ccttgaacca gcgcagctca tccacgctgc cgttgcgcgg catgatgccg atgtggacgc
     1681 ccttgtctgg ctcccaggcg tagacgggtc ccccctgcat ggcccgctcc atggatccgg
     1741 tcaggggcat gatggggaag atcacgtgat cccgggtgat catgaagtcg tgaaccatgg
     1801 acgcgtaggg cgctttgaac cactcggagc gggtgaggtt tccgtcgccg tccaccacgt
     1861 ggaaggccat gtcctcggag atgaggccct tggcgttgta gccgaacatg agcatctcac
     1921 cggtctcgca gtcgatcttg gggtgcgccg tcatcgggcc ttccagcttg ccgtcgtagc
     1981 gccaggctcc cttcgattcc agggtgtaag gatccagctc gaacggagcg tgtgcctcct
     2041 cgagggccag cagcttgccg ccatgccaca ccacgttggt gtttgccagg ccgtcggtct
     2101 ccatgccgcg tacgctctcg tcggagtcca tggggttgaa cgcgctgaag agcgccccgc
     2161 cggcttcgtg ttccctggtc cacttgacgg tacgcaccca
//
LOCUS       DBNHLM010000088         6982 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_911146, whole genome shotgun sequence.
ACCESSION   DBNHLM010000088 DBNHLM010000000
VERSION     DBNHLM010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6982)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6982
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_911146"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="AC16QF_04215"
     CDS             complement(<1..404)
                     /locus_tag="AC16QF_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6447788.1"
                     /translation="MRFTEDDLAFQAEVRSFIDRHWPADVRLARATVPAHGLDRPAEE
                     QAWFDALVARGWSVPNWPLEHGGTDWTPTQRFIWDRETVRAETPQMSVFGVTMLGPIL
                     CRWGSVDQQEKFLPPIREARVQWCQGYSEPGAG"
     gene            603..1226
                     /locus_tag="AC16QF_04220"
     CDS             603..1226
                     /locus_tag="AC16QF_04220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6447789.1"
                     /translation="MQDDALPPQLFARADESPDSAFYREPRFVTHIDDATIDCLTAFY
                     RTFIPENAAVLDLMSSWVSHLPPECSYARVAGLGMNEAELAANPRLDDHVVHDLNAAP
                     ELPYPDSAFDRVILAVSIQYLTRPVEVLREALRTLREDGEICIAMSHRLFPTKAILAF
                     HRLGPADRVRLVSTYLELAGFLDVRFVDRSPDGADPLWLVVGRRAGR"
     gene            complement(1228..1890)
                     /locus_tag="AC16QF_04225"
     CDS             complement(1228..1890)
                     /locus_tag="AC16QF_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447790.1"
                     /translation="MAETEAYTFTAALTVCLDITQPESYLAVPGVRELAADLGMDVRW
                     LPYSVPPWPARSEPGPEADRGTWHRWHRARYRERDLRRYARLLSIDLGDLHRQIKGEL
                     AGLALLWVQDQPAAVGGALLDGLLEGHWSGALNIEEPASVTAMLVQAQARVAGWDEYR
                     SGRGPVELAALRSALSAAGVFDVPSLVVAHAVPEPEVFLGRAHLPMVRWLLTDRQGAP
                     PI"
     gene            complement(1883..2599)
                     /locus_tag="AC16QF_04230"
     CDS             complement(1883..2599)
                     /locus_tag="AC16QF_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR6447791.1"
                     /translation="MPSTSSFDPLGSSSGLIVYVDFKSPYAYLAKDPTFQLADDLGID
                     LDWRPLTLDINSYLGSARLNASGAVAESRRTPEQWHRVRYAYRDARRYANLVGLTVRG
                     TTKIWDSSLASIGLQWAKGQGAGPLRAYIDLTYERFWKRELDIEDASVVAGVLAEAGA
                     DPDGFVDFARGEGRAEHDVMQQRIFAAGIFGVPSYVLDGELYFGREHLPRIRWLLTGR
                     PGPCPDVAYRHFDAQETIRG"
     gene            2724..3581
                     /locus_tag="AC16QF_04235"
     CDS             2724..3581
                     /locus_tag="AC16QF_04235"
                     /inference="COORDINATES: protein motif:HMM:NF006109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIR6447792.1"
                     /translation="MAYNTILYDVEDGVLTLTLNRPERLNAFNHEMLSEMIDAFDRAD
                     ADDEVSSIIVTGAGRAFCAGADLGAGGDTFNADGREDRASGLHPDGGGRLTLRIYELK
                     KPIIAAVNGPAVGVGVTMTLAMDIRLAADVAKFGFVFARRGIVPEACSSYFLPRIVGI
                     SQALEWCYSGRVFPAEEALAGGLVRSLHSKEELMPAARALACEIADNTSAISVTLVRQ
                     MMWRMLGADHPMEAHKIDSRGVYFTGKSPDAREGVEAFLEKRPARFPGKVSSDLPEFF
                     PWWEERTFS"
     gene            3592..3831
                     /locus_tag="AC16QF_04240"
     CDS             3592..3831
                     /locus_tag="AC16QF_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HIR6447793.1"
                     /translation="MSSPTIAQKAPYAVDVQAGRKYFWCACGRSAKQPFCDGSHEGTA
                     LTPLPYTAEKDATLYFCGCKHTKNVPLCDGSHNDL"
     gene            3847..6636
                     /locus_tag="AC16QF_04245"
     CDS             3847..6636
                     /locus_tag="AC16QF_04245"
                     /inference="COORDINATES: protein motif:HMM:NF015926.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HIR6447794.1"
                     /translation="MLSRIFGNKDPLSQPDPAARRRAVEGLSEGKAGELQERLLELAR
                     SDADPGVRQACVSRLQGTSDLAQLLDDGLMANRAAERIAELLGPDASSELTRHPLVLR
                     ARLFRLAPEDAARLIDSIEDEDTLVDLTAGSREPLRGRILAGIRSARALAALEQQSRG
                     RDKGLNRFAREGMERIKQLRREAQATLDRLNELADALERASRQAPGIAARQRYESLLR
                     EFDQAYERFAGHARALEGCNEPPRDASPIRARVAALVAPAEPVAISRQEETPASGGEA
                     FADLVPQLEALEGLMEAGHDFQTVSETRSRLTEAWLRAADHALPGDAEHRVFERVSHA
                     YQNLADAAGRLTAAGWQTHRDPLPSDLPDDPGEAAGFWQAAEERRRLLKRAEGLVRAV
                     AWPDWASPTAELRDLLDDVERLREEVARSDALGSSRHEDLKMLISTAASAIDEGAVRA
                     ATEALGRARAILQALPARGREAQARALQQESARLSELRDWQTFATTPKREALCEAVQN
                     LADQPLDPPDQAERIKALRGEWNGLGPVSGREDRALADRFNRLAEQAFEPCRAYFAEQ
                     ASVRASNLVERGKICDQLENYLESTDWKAADIKAAERILRAARDEWRRFQPVDRNPGK
                     PVEERFEVLQERLHKLIKAEWDANLQLKRQIVEEAEALAGSEADPHDRAQEAKALQRR
                     WREIGITPRGPDRKLWQQFRGACDAIFDALGAAREASHQAVSEALDAADNLLTSFESD
                     LGNTSATEAEAEILTGFRRRFSELPPLPEREGRQISRRFEELTRSYRSLLHQKSRYQE
                     RAELDRLRALDEAVTHTEIAPGLTAEPTQDAIADPVVSERLQKSGEPIPMDALRALTV
                     RAEILAGAESPAQDREIRLKLQVEEMNVGMGSQRRAQDPLEMAREWCARGPKDEACSA
                     LRERFFDALARALED"
     gene            complement(6633..>6982)
                     /locus_tag="AC16QF_04250"
     CDS             complement(6633..>6982)
                     /locus_tag="AC16QF_04250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6447795.1"
                     /translation="AEALPENLVVRGDERDLMEMLGNVLENAFKYTTARIRIGAERNS
                     VVSVWVEDDGPGIAPELRQEVLNRGSRADEMRPGQGIGLSVVAELVALYQGELRIETS
                     ALGGARICLDLPA"
BASE COUNT         1247 a         2351 c         2337 g         1047 t
ORIGIN      
        1 cccgctccgg gctccgagta gccctggcac cattggacgc gggcttcccg aatgggcggg
       61 agaaactttt cctgctgatc gacgcttccc caacggcaca ggatgggtcc cagcatcgtt
      121 acgccgaaca cgctcatctg gggcgtttcc gcccgcacgg tttcgcgatc ccagatgaac
      181 cgctgggtgg gcgtccagtc ggtaccgccg tgctccaagg gccagttcgg aacgctccaa
      241 ccgcgcgcca cgagcgcgtc gaaccaggcc tgctcctcgg cggggcgatc gaggccgtgg
      301 gcgggcacgg tggcccgggc caggcgaacg tcggcaggcc agtgccgatc gatgaacgaa
      361 cgcacctcgg cctgaaaggc aaggtcgtct tcggtgaatc tcatgtccat ggtggtcccg
      421 cgaaagtccc cgagatgcga aaccgcggaa tcttatcagc tcctatctgc gcggtcttgg
      481 ggcccggatc atcgggtaac ggaggccggg ctcgcctccg aggacagggc aggccgcggg
      541 caacatgcca ttggccgcat tcgctagaat gacggcccaa gcagcaacgg ttcggatcca
      601 ccttgcagga cgacgccctt cccccgcagc ttttcgcccg ggccgacgag tcaccggatt
      661 cggccttcta tcgcgaaccg cgcttcgtga cgcacatcga cgatgccacc atcgactgcc
      721 tcacggcctt ctaccgcacg ttcatccccg agaacgccgc ggtcctggac ctgatgtcgt
      781 cctgggtatc ccacctgccg ccggagtgca gctacgcgcg cgttgccggt ctcggcatga
      841 acgaggcgga gctggcagcc aacccgaggc tcgacgacca tgtggtgcac gatctcaacg
      901 ccgcaccgga gctgccgtac ccggacagcg cgttcgaccg cgtcatcctg gcggtttcga
      961 tccagtacct gacacgccct gtggaggtgc tgcgcgaagc cctgcgcacc ctcagagagg
     1021 atggcgagat ctgcatcgcc atgtcccacc gcctgtttcc aaccaaggcg atcctcgcct
     1081 tccacaggct cggccccgcc gaccgcgtgc ggctggtatc cacctatctg gagctcgccg
     1141 gttttctgga cgtgcgcttc gtcgaccgat cccccgacgg cgcagatccc ctgtggctgg
     1201 tcgtcggacg tcgcgccggg cgctgaatca gatgggcggc gcgccctgcc gatccgtgag
     1261 caaccagcgg accatcggca gatgcgcgcg accgaggaag acttcgggct cgggcaccgc
     1321 gtgggccacg acgagcgaag ggacatcgaa aacccccgct gcgctgagcg cggagcgcag
     1381 ggctgccagc tcgacggggc cgcgcccgct gcgatactcg tcccagccgg caacgcgggc
     1441 ctgagcctgg acgagcatcg cggtcaccga tgccggctcc tcaatgttca gcgccccgct
     1501 ccagtgaccc tccagcaggc cgtcgagcaa agcgccgccc acggcggccg gctgatcctg
     1561 cacccagagc agcgcgagcc cggcaagctc gcccttaatc tgccggtgca ggtcccccag
     1621 atcgatgctg agaagacggg cgtagcggcg caggtcccgc tcccgatatc gcgcacgatg
     1681 ccagcggtgc caggttcccc gatccgcttc ggggcccggc tcggaccgtg caggccaagg
     1741 cggcaccgaa tagggcaacc atctcacgtc catgcccaga tcggcggcga gctcgcgcac
     1801 gcccggaacg gccagatagg actccggctg ggtgatgtcc aggcagacgg tgagcgcggc
     1861 ggtgaacgtg taagcctcgg tctcagccac ggatcgtttc ttgcgcatcg aagtgacgat
     1921 aggccacgtc cggacagggg cccggtcggc ctgtaagcaa ccagcgtatt ctcggcaggt
     1981 gttccctgcc gaagtagagc tcgccgtcga gcacatagct cggcacgccg aagatgccgg
     2041 cggcgaagat acgctgctgc atgacatcgt gctcggcacg cccctcgccg cgggcgaagt
     2101 cgacgaaccc gtcggggtcc gctcccgcct ctgcgagcac gccggcaaca acggatgcgt
     2161 cctcgatgtc gagctcgcgc ttccagaacc gctcgtaggt cagatcgata taggcccgca
     2221 gcgggccggc tccctgccct ttggcccact gcagaccaat tgatgcaagg gacgaatccc
     2281 agatcttcgt ggtgcccctg acggtaaggc ccaccaggtt cgcgtaacgc cgggcatcgc
     2341 gataggcgta cctgacccgg tgccattgct cgggcgtacg gcgactctcg gcaaccgcac
     2401 ccgaggcgtt caaccgcgca gagcctaagt agctgttgat gtcgagcgtg agcggccgcc
     2461 agtccaagtc gatgccgaga tcgtcggcca gctggaatgt ggggtccttg gccaggtagg
     2521 cataagggct cttgaagtcg acgtagacga tgagccccga tgagctgccg agcgggtcga
     2581 aagaggatgt ggacggcatt cgggcgattg tagtaggggt tgcgcggcgc gccaaaccag
     2641 gtcgcatgcc agtccgcatg gaaaatccgc aggaatctag gcaacatgcg ccactgtcta
     2701 ctcatctggg gacgcccccg cacatggcct acaacacgat cctttacgac gtcgaagacg
     2761 gcgttctgac gctgacccta aatcggccgg aacgtctgaa cgcgttcaac cacgagatgc
     2821 tcagcgagat gatcgacgcc ttcgaccggg cagatgcaga cgacgaggtg agcagcatca
     2881 tcgtcacagg ggccggtcgg gcattctgtg ccggcgcgga cctcggcgcc ggcggcgaca
     2941 ccttcaatgc ggacggtcgc gaggaccgag ccagcggcct tcacccggac ggcggcggcc
     3001 gcctgaccct gcggatctac gagctcaaga agccgatcat cgccgcggtg aacgggcccg
     3061 ccgtcggcgt gggcgtgacc atgacgctgg ccatggacat ccgcctggcc gcagacgtgg
     3121 ccaagttcgg cttcgtcttt gcacgccggg gcatcgtccc cgaagcctgc tcgagctact
     3181 tcctgccacg catcgtgggc atcagccagg cactggaatg gtgctacagc ggccgcgtgt
     3241 tccccgccga ggaggctctg gctggcgggc tggtgcgcag tctgcactcg aaggaggagc
     3301 tgatgccggc agcaagagcc ctcgcctgcg agatcgccga caatacttcc gcgatctcgg
     3361 tgacgctggt gcgccaaatg atgtggcgaa tgctgggcgc ggatcatccc atggaagccc
     3421 acaagatcga ctcccgtggg gtgtacttca ccggcaagtc tccggacgct cgggagggcg
     3481 tcgaagcgtt cctggaaaaa cggcccgccc ggttcccggg caaggtcagc agcgatctgc
     3541 cggaattctt cccctggtgg gaggagagaa cgttttcatg aggacaacga catgagctca
     3601 ccgaccattg cccagaaagc accctacgcg gtagacgtgc aggccggccg caagtatttc
     3661 tggtgcgcct gcggacgcag cgccaagcaa cccttctgtg acggttccca cgagggcacg
     3721 gcgctgaccc cgctgccgta cacggcggaa aaggacgcta cgctctactt ctgcggttgc
     3781 aagcacacca agaacgttcc cctctgcgac ggctcccaca acgacttgtg atcgtgaagg
     3841 atcgccatgc tgtcgcgaat cttcggaaac aaggaccctc tgagccagcc ggatccggcg
     3901 gcccggcggc gcgcggtcga ggggctgtcg gaaggtaaag cgggcgagct gcaggagcgg
     3961 ttgctcgagc ttgcgcgcag cgacgcagat cctggcgttc gccaggcctg cgtgagccgg
     4021 ctgcagggaa catcggacct cgcgcagctg ctcgacgacg ggctgatggc gaaccgtgca
     4081 gctgagcgga tcgccgagct gctcggcccc gacgcctcca gcgagctgac cagacacccg
     4141 ctcgtccttc gcgcccgcct gttccgcctg gcgcccgagg acgcagcccg cctgatcgat
     4201 tccatagagg acgaggacac gctggtggat ctgacggccg gctcgcggga gccgctgcgc
     4261 ggaaggattc tggcgggaat ccgatcggcc agggcgctgg cggctctgga gcagcagagc
     4321 cgcggtcggg acaaggggct caatcgcttc gcccgggaag gcatggagcg catcaagcag
     4381 ctcaggcgcg aggcgcaggc cacgctggac cgcctgaacg agcttgccga cgccctcgag
     4441 cgcgcatcgc ggcaggcgcc cggcatcgcc gcacgacaac gctacgagtc actgcttcgg
     4501 gagttcgatc aggcttacga gcgttttgcc gggcacgccc gggcgctgga gggttgcaac
     4561 gaaccgcctc gggacgcgtc acccatccga gcccgcgtgg ccgctctcgt cgcccccgcg
     4621 gaaccggtgg cgatcagccg gcaggaggaa acacccgcga gcggtgggga ggcgtttgcc
     4681 gacctcgtgc cgcagctgga agcgctggag gggctgatgg aagcggggca cgacttccag
     4741 acggtatccg agacccggtc ccgtctgacc gaagcctggc tgcgcgccgc cgaccacgcg
     4801 ctgccgggcg atgcagagca tcgggtgttc gagcgcgtct cccacgccta tcagaacctg
     4861 gccgatgccg ccgggcggct gacggcggcc ggctggcaga cgcatcgtga tcccctgccc
     4921 agtgacctgc ccgatgatcc gggggaagca gccggcttct ggcaggcggc ggaggagcgg
     4981 cgcaggttgc tcaagcgcgc ggaggggctc gttcgagccg ttgcctggcc ggattgggcg
     5041 agccccacgg ctgagcttcg agacctgctc gacgacgtgg aacgactgcg ggaggaggtg
     5101 gcgcggtcag acgccctcgg cagcagtcgg cacgaggacc tcaagatgct gatcagcacc
     5161 gcggcgagcg ccatcgatga gggcgccgtt cgagcggcca cggaggccct cggccgcgcc
     5221 cgggcaattc tgcaggccct tcccgcgcgt gggagggaag cccaggccag agcccttcag
     5281 caggagtcgg ccaggctgtc cgagctgcgt gactggcaga ctttcgcgac cacgccgaag
     5341 cgggaggccc tttgcgaagc cgttcagaac ctcgccgatc agcccctgga tccgccggat
     5401 caggccgagc gaatcaaagc gctgcgcggc gagtggaacg gtctcgggcc cgtgtccgga
     5461 cgcgaagatc gcgccctcgc cgatcgtttc aaccgcctcg ccgagcaggc attcgagccg
     5521 tgccgagcct acttcgccga gcaggccagc gtacgcgcaa gcaacctggt cgagcgcggg
     5581 aagatctgcg atcagctgga aaactacctg gaaagcaccg actggaaagc ggcggacatc
     5641 aaggcggccg agagaattct gcgcgccgcc cgggatgagt ggcgcagatt ccaacccgtc
     5701 gaccggaacc cgggcaaacc cgtcgaggag cggttcgagg tgctccagga acggctccac
     5761 aagctcatca aagccgagtg ggatgccaac ctgcagctga aacggcagat cgtcgaggag
     5821 gcggaagcac ttgccggcag cgaagccgac ccccacgacc gggcccagga ggcaaaagcg
     5881 ctgcaacggc gctggcggga gataggcatc acgcccagag gccccgaccg aaagctgtgg
     5941 cagcagtttc gcggcgcgtg tgatgcgatc ttcgatgcgc tgggagccgc ccgggaggca
     6001 tcccaccagg cggtcagcga ggccctcgac gctgctgata acctgctgac ctcctttgag
     6061 agcgaccttg ggaatacgtc agcaaccgag gccgaggcag agatcctcac cggcttccga
     6121 cgccgcttca gcgagctgcc gccactgccg gagcgggagg ggcggcagat ttcccggcgc
     6181 ttcgaggagt tgacccgcag ctaccgatcc cttctgcatc agaagtcccg ctaccaggag
     6241 cgggccgagc tcgatcgcct cagagccctg gacgaggcgg tgacccacac ggagatcgcg
     6301 ccgggcctga cggcggagcc gacgcaagat gctatcgcgg atcccgtcgt ctccgagcgt
     6361 ctgcagaaaa gcggcgaacc gatccccatg gacgcccttc gcgcgctcac cgtgcgggcg
     6421 gagatcctcg ccggcgcgga atccccggcc caggaccggg agattcgctt gaagcttcag
     6481 gtcgaggaga tgaacgtcgg gatgggcagc cagcggcgtg ctcaagaccc tctggagatg
     6541 gcccgcgagt ggtgcgcccg cggtcccaaa gacgaggcgt gctcggcgct gcgcgagcgg
     6601 ttcttcgacg cgctcgcacg ggcgctggag gactaggcgg gcaggtccag gcagatgcgg
     6661 gcgcccccca gggcgctggt ctcgatacgc aactctccct gatagagcgc cacgagctcc
     6721 gcgacgacgg acaggccgat cccctgtccg gggcgcatct cgtcagcgcg gctgccacga
     6781 ttgagcacct cctgacgcag ctccggggcg ataccgggcc cgtcgtcttc cacccacacc
     6841 gacaccaccg agttgcgctc tgcgccgatg cgtatccgcg ccgtggtgta tttgaaggcg
     6901 ttctccagca cgttgccgag catctccatc agatcacgct catcgccccg aaccacgagg
     6961 ttctcgggaa gcgcttcggc aa
//
LOCUS       DBNHLM010000089         6180 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_671292, whole genome shotgun sequence.
ACCESSION   DBNHLM010000089 DBNHLM010000000
VERSION     DBNHLM010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6180)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6180
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_671292"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="AC16QF_04255"
     CDS             <1..1078
                     /locus_tag="AC16QF_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR6447796.1"
                     /translation="LVAVPVISFLVIPAALIGALLMELVPFLGEAALGMAAVVVAQLM
                     GVLEASASLPPLVAAPRLLPVFVALATGLVMLACGGSGRHLLLCLAWAVWALPPDHGL
                     PRGEFRVLAMDVGQGSAVLVDTKRHRLLFDAGPRYPSGFDLGAQVVLPTLVATGSPRL
                     DALVISHADIDHSGGKGAVVGAVPVGEVYESRPDSESSRCSAGQSWRWDGVDFRFLHP
                     PPLARARGLGSNDASCVLLIDNGRERALLPGDIGAAVEGQLLRALDPVDVLLAAHHGS
                     ASSSSRAFVRVLEPDVVFISAGRRNRYGHPHPDVVGRFAEVGARVHSTAEHGALVWDS
                     RTPGSVRRWRRDTPRYWVQGSQAD"
     gene            complement(1104..1862)
                     /locus_tag="AC16QF_04260"
     CDS             complement(1104..1862)
                     /locus_tag="AC16QF_04260"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR6447797.1"
                     /translation="MEQRKHPRVQLPLLVELHHPSLPQGKLRCVARDVSKGGVYVHFD
                     QPPTLIPGAKLRLTLLNPSQVESQPTPTVEMMVARTEPTGLGLAFVNATSQHLWASVE
                     RLRDELAIGRDYFQVFQGAVVLNEQDEVLLVQQHGKWGFPGEYLVVGEDAETALKAML
                     TRRFALADLTIDGVLKVGSGATIDLPEAAVFRVYHRVTTSTAEISASAEEAYRKVRWV
                     ENRRSVAQMAFVSKELRELANAALLWAAGRRRAG"
     gene            1929..4496
                     /gene="clpB"
                     /locus_tag="AC16QF_04265"
     CDS             1929..4496
                     /gene="clpB"
                     /locus_tag="AC16QF_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIR6447798.1"
                     /translation="MRPDKMTSRLQNALADAQSMALGKDHAAIEPLHLLAVLVEQKAG
                     SVLPQLVRAGARANELQTRLNERLGQLPTLKSPTGEMSFAPAFVKVLNLADRLAQKAG
                     DDFISTEQVLLALFEDPTVESVLRESGVDKAALEAIVSQARSGETVSDPNAEEHREAL
                     EKYTVDLTAQAEEGKLDPVIGRDDEIRRTVQVLQRRTKNNPVLIGEPGVGKTAIVEGL
                     AQRILNGEVPEGLKNKRILALDMGSLIAGAKFRGEFEERLKAVLNELSKQEGNVILFI
                     DELHTMVGAGKAEGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQK
                     VLVDEPSVEDTIAILRGLKERYELHHAVDITDPAVVAAARLSHRYITDRQLPDKAIDL
                     IDEAASRIRMEMDSKPEAMDRLERRLIQLKMEAEALKQESDDASRKRLEDLQSSIAEL
                     EREYADLEEIWNAEKASLRGAQDLKEDLDAARGEFEVARRAGDLARMSELQYGTIPEL
                     EKQLAAASNGEAEPRQLLRNSVTEEEIGEVVSKWTGIPITKLLEGERDKLLRLEEALH
                     ERVVGQDEAVRIVADAVRRSRAGLSDPDRPNGSFMFLGPTGVGKTELCKALAQVLFDS
                     DEALVRVDMSEFMEKHSVARLIGAPPGYVGYEEGGFLTERIRRRPYSLILLDEIEKAH
                     PDVFNILLQVLDDGRLTDGHGRTVDFRNTVLVMTSNLGSEAIQSLTEAGNADRIKDTV
                     LGVVSQHFRPEFLNRVDDIVVFHPLGREHVAEIAKLQFDILRRRLEEQDLTIDIAPAA
                     VEALVEEGYDPVYGARPLKRVIQRRIENPLASRILGGDFLPGSTIRVGYADGDYSFD"
     gene            4563..4835
                     /locus_tag="AC16QF_04270"
     CDS             4563..4835
                     /locus_tag="AC16QF_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447799.1"
                     /translation="MNDRFESLPLFPDEAPPAKRRQVDAGRSEPGRGRASAAARSLGS
                     RDPHLIAANRLARAIRRLPDHSPEQTRTGQAICRHLLALLEEAERR"
     gene            4970..5806
                     /gene="galU"
                     /locus_tag="AC16QF_04275"
     CDS             4970..5806
                     /gene="galU"
                     /locus_tag="AC16QF_04275"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HIR6447800.1"
                     /translation="MIRKCLFPVAGYGTRFLPATKAMAKEILPVLNKPLVQYAVEEAL
                     EAGLTDVGFVTGRGKRAIEDHFDTSYELEHQIAGTGKEKALDGIRRIMEECTFSYTRQ
                     VNMRGLGDAILKGRTLVGENPFGVVLADDLCFGNGHREGGVLKQMVKLFKQFRCSIVA
                     IEEVPEDRVSSYGVIAGEALSDRLVRITDMVEKPSPENAPSNFGIIGRYILTPDIFEI
                     LENTEPGKNGEVQITDALLQQAKDGCVIAYRFQGKRFDCGSVAGFVDATNYCYEHLYT
                     ET"
     gene            5815..>6180
                     /locus_tag="AC16QF_04280"
     CDS             5815..>6180
                     /locus_tag="AC16QF_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447801.1"
                     /translation="MSFDVALKLLNERSWKPVPLAEPIPVSSLPTPALLIDESALDRN
                     IKRMADFVAARGKNARPHGKTHKCPIIAARQIEAGAVGICAAKVSEAAVFVNAGVEPV
                     LITSPVTTAAKAELIVALAR"
BASE COUNT         1099 a         1923 c         2052 g         1106 t
ORIGIN      
        1 cctcgtcgcg gtgcccgtca tcagctttct ggtcattccg gcggcgctga tcggcgcgct
       61 gctgatggag ctggtcccgt tcctcggcga ggctgcgctc gggatggctg ccgtggtcgt
      121 cgcgcagctg atgggcgttc tcgaagcgtc ggcgtcgctg cccccgctcg tggccgctcc
      181 acgactgttg ccggtgttcg tggccctggc taccgggctc gtcatgctgg cgtgtggcgg
      241 ttcgggcagg caccttttgt tgtgcctcgc ctgggcggtc tgggcgctac cccctgatca
      301 tggcttgccc aggggtgagt tcagagtcct cgcgatggac gtggggcagg gcagcgcagt
      361 gctggtggac acgaagaggc atcggctgct gttcgatgcg ggcccgcgct atcccagcgg
      421 cttcgacctg ggggcccagg tcgtcctacc caccctcgtt gccaccggct ccccgcggct
      481 ggacgccctg gtgatcagtc atgcggatat cgatcacagc ggcggcaagg gcgccgtggt
      541 gggcgccgtg cccgtaggtg aggtatacga atcccggccc gactcagaat cctcacgctg
      601 ctcggctggc cagtcctggc gttgggacgg cgttgatttc cgcttcctgc acccgccgcc
      661 gctggcacgc gctcgcggcc tggggagcaa tgacgcatcc tgcgtgctgc tcatcgacaa
      721 cggccgggaa cgcgcgctgc tgcctggaga catcggtgcc gccgtagaag ggcaactgct
      781 gcgagcactg gatccggtgg acgtccttct ggccgcccac cacggaagcg cttcctcgtc
      841 gtccagggct ttcgtgcgcg tgctggagcc ggacgtggtc ttcatcagcg ccggccgacg
      901 taaccgctac ggccatcctc atcccgatgt ggtcggacgc tttgcggagg tcggcgcgcg
      961 cgtccactca accgcggagc acggggcgtt ggtatgggac agccggacgc ctggctcggt
     1021 gcggcggtgg cggcgggata cgccgcgcta ctgggtgcag ggatcgcaag cggactgacg
     1081 cgccggtcgc ccccttggcc tgttcagccg gcgcgccggc gcccggctgc ccagagcaac
     1141 gcggcgttgg cgagctcgcg cagctctttg ctgacgaatg ccatctgggc aacgctgcgt
     1201 cggttttcca cccagcggac cttgcgatac gcttcctctg cgctggctga gatctccgcc
     1261 gtcgaggtgg tcacccggtg atagaccctg aagacggctg cctcgggcag gtcgatcgta
     1321 gccccgctgc caaccttgag gaccccgtcg atcgtcaggt cagccagagc gaaacggcgg
     1381 gtcaacatcg ccttcagcgc cgtttccgcg tcctcgccga cgaccaggta ctcgccggga
     1441 aacccccact ttccgtgctg ctgtacgagc aacacctcat cctgctcgtt gagtacgacc
     1501 gcgccctgga acacctggaa gtagtcccgg ccgatcgcca gctcgtcacg cagacgctct
     1561 acgctagccc acaggtgctg actggtggca ttgacgaagg caaggcccag acccgtcggc
     1621 tcagtccgcg ctaccatcat ctccacggta ggcgtgggtt ggctctccac ctggctgggg
     1681 ttcagcaggg tcagtcgcag cttggcgccg ggtatgaggg tgggaggctg gtcgaaatgg
     1741 acgtaaacgc cccctttgga gacgtcccgg gcaacgcagc ggagcttgcc ctggggcagc
     1801 gacgggtggt gcagctccac caggagggga agctggaccc tcggatgctt tctctgctcc
     1861 atggggccat tgaaatctcg aaaatcggac gcattgtagc agcaggtttt cgttgaggta
     1921 gcgttgggat gcgcccggac aaaatgacaa gcagactgca aaatgcccta gcggacgcgc
     1981 agtccatggc tctaggtaag gaccacgcag cgatcgagcc gctgcacctg cttgccgttc
     2041 tcgtcgagca gaaagcgggc tccgtcctgc cgcagctggt gcgagccggc gctcgcgcga
     2101 acgagctgca gacgcgtttg aacgaacggc ttggccagct gccgacgctc aagagcccca
     2161 ccggcgaaat gtcttttgcc ccggcgttcg tgaaggtcct gaacctggcc gatcgcctgg
     2221 cccagaaagc gggcgacgat ttcatctcca cggagcaggt gctccttgcg ctgttcgagg
     2281 acccgacggt cgaatcggtt ctgcgcgaat cgggcgtgga caaggcggcg ctcgaggcga
     2341 tcgtgtctca agcccgcagc ggggagacgg tctccgatcc caatgccgag gagcatcgcg
     2401 aggcgctgga gaagtacacg gtggacctca cggcccaggc cgaggagggt aagctcgacc
     2461 cggtgatcgg gcgtgacgac gagattcgcc gcacggtgca ggtgctgcag cgtcgaacga
     2521 agaacaaccc ggtgctcatc ggcgagcccg gcgtgggcaa aacggccatc gtcgagggac
     2581 tggcccagcg gatcctcaat ggtgaggtgc cggaagggct caagaacaag cgcatcctcg
     2641 ccctggacat gggttctctc atcgctggag cgaagtttcg gggtgagttc gaggagcggc
     2701 tcaaggcggt tctcaatgag ctctccaagc aggagggcaa cgtcatcctc ttcattgacg
     2761 agctccatac catggtcggg gcaggtaagg ccgagggcgc gatggatgcg ggcaacatgc
     2821 tcaagccggc cctggctcgc ggcgagctcc actgcgtggg cgcgacgacc ctggacgagt
     2881 atcgccagta catcgaaaag gatgccgcgt tagagagacg ctttcagaaa gtgctcgtcg
     2941 acgagcccag cgtggaggac accatcgcca ttctcagggg cttgaaggaa cgttacgagc
     3001 ttcaccacgc ggtcgacatc actgacccgg ccgtcgttgc ggcggccagg ttgtctcatc
     3061 ggtacatcac cgaccgtcag ctgccggaca aggccatcga tctgatcgac gaggcggcga
     3121 gccgcatccg catggaaatg gattccaagc cggaagccat ggaccggctg gagcgccggc
     3181 tcatccagct gaagatggag gccgaggcgc tcaagcagga aagcgacgat gcgagccgaa
     3241 agcgcctgga ggacctgcag agctccattg ccgagctcga gagagagtac gcggatctag
     3301 aagagatctg gaacgcggaa aaggcgtcct tgaggggcgc tcaggatctc aaggaggatc
     3361 tggacgcggc acgaggcgag ttcgaggtgg cgcgccgcgc cggcgatctt gccaggatgt
     3421 cggagctgca gtacggcacg atcccggagc tcgagaagca gctggcggct gccagcaacg
     3481 gcgaggccga gcctcgccag ctgctgcgca acagcgttac cgaagaagag atcggagagg
     3541 tcgtgtcgaa gtggacgggc atcccgatca ccaagctgtt ggaaggtgag cgcgacaagc
     3601 tcctgcgtct ggaagaggcc ctgcacgaac gcgtcgtggg ccaggacgag gcggtgcgca
     3661 tcgttgccga tgccgtccgc cgctcccgtg ccgggctgtc ggatcccgac cgccccaacg
     3721 gctcgttcat gttcctcggc cccaccggcg tgggcaagac cgagctgtgc aaagcgttgg
     3781 cacaggtgct cttcgacagc gacgaagcgc tcgtgcgcgt ggacatgtcc gagttcatgg
     3841 agaaacactc cgttgcgcgg ctcatcggcg cccctccggg atacgtaggc tacgaggagg
     3901 gcgggtttct caccgagcgg atacgccgcc ggccctactc gctgatcctg ctcgatgaga
     3961 tcgaaaaggc gcaccccgac gtgttcaaca ttctgctgca ggtgttagac gatgggcgtc
     4021 tcacggacgg gcacgggaga acggtcgact ttcggaacac ggttctggtc atgacgtcga
     4081 acctcggctc cgaagccatt cagtcgctca cggaggcggg caatgccgac cgaatcaagg
     4141 atacggtcct gggcgtggtc tcgcagcact tccgccccga gttcctgaat cgggtggacg
     4201 acatcgtcgt gtttcacccg ctcgggcgcg agcacgttgc cgagattgcc aagctccagt
     4261 tcgacattct gcgccggcgg ttggaagaac aggacctcac gatcgacatc gctccggccg
     4321 cggtggaagc gctggtggag gagggctacg acccggttta cggtgcccgg ccactcaagc
     4381 gcgtgattca aagacgcatc gagaaccccc tggccagccg gattctgggg ggcgacttcc
     4441 tcccggggtc caccatccgg gtcggttatg cggacggtga ctactcattc gactaataac
     4501 ctgtcgaaaa acgatgtgcc gagtattgta tgtttataca gtattcggta catttccacg
     4561 tcatgaatga ccgctttgaa tccctgccgc tgttcccgga cgaggcgccg ccggcgaagc
     4621 gtcgccaggt ggacgcaggc cggtctgaac cggggcgagg ccgcgcgagc gcagccgccc
     4681 ggagcctggg ttcgcgggat cctcatctga ttgcggccaa ccggctcgcc cgagccattc
     4741 gccggctccc cgaccactca ccggagcaga cgcggaccgg ccaggcgatc tgtcggcacc
     4801 ttctggccct cctggaggaa gccgagcgtc gctgatcctc ccgcattcac cgtcgggtca
     4861 ggttgtcgct ccgccgtcgt ttgttgttca atctgtgcgg ttcgtccatg gtagtggacg
     4921 ctaaccgtcc taccatcgtc atttgtactg attctttgag atggatttca tgatcaggaa
     4981 gtgtttgttt cccgtcgccg gttacgggac ccgttttctg ccggcgacca aggcgatggc
     5041 caaggaaatc cttcccgtcc tcaacaagcc tctcgttcag tacgcagtgg aggaagccct
     5101 ggaggccggt ctcaccgacg tcggcttcgt gacggggcgg ggcaagcgcg ctatcgagga
     5161 ccacttcgac acgagctatg agctggaaca tcagatcgcc gggaccggca aggaaaaggc
     5221 cctggacggt atccgccgga tcatggagga gtgtacgttc tcgtacacgc ggcaggtcaa
     5281 catgcggggc ctgggggatg cgattctgaa aggacgcacg ctggtcggcg agaacccctt
     5341 cggcgtggtg ctggccgacg atctgtgttt cggcaacggc caccgcgagg gcggggtcct
     5401 caagcagatg gtgaaactct tcaagcagtt tcgctgcagc atcgtggcga tcgaggaggt
     5461 gcccgaggac cgggtgagca gctatggcgt catcgccggg gaagcgctta gcgaccgcct
     5521 ggtacgcatc acggacatgg tggagaagcc gagtcctgag aatgcgccca gcaacttcgg
     5581 catcatcgga cgctacatac tcacccctga cattttcgag attctggaga acacggagcc
     5641 cggcaagaac ggtgaggtcc agatcacgga cgcgcttctc cagcaggcta aggacggctg
     5701 cgtcatcgcc taccggttcc aggggaagcg cttcgactgc ggttcggtgg ccgggttcgt
     5761 cgacgcgacc aactactgct acgagcatct ctatacggaa acctagccag gcgcatgtcg
     5821 tttgacgttg ctttaaagct gctcaacgag cgatcctgga agccggtccc gcttgccgag
     5881 ccgatcccgg tcagctccct gccgacgccc gcgttgctga tcgacgagag cgcgctggat
     5941 cgcaacatca agcgcatggc ggacttcgtc gccgccaggg gcaaaaacgc ccgcccacac
     6001 ggcaaaaccc acaagtgtcc gatcatcgcg gcgcggcaga tcgaagccgg tgccgtgggg
     6061 atctgtgcgg cgaaggtttc cgaagccgcc gtcttcgtca atgccggcgt ggaacctgtt
     6121 ctgatcacct ctcctgtcac taccgcagcg aaggccgagc tcatcgttgc gcttgcccgg
//
LOCUS       DBNHLM010000090         5972 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1330168, whole genome shotgun sequence.
ACCESSION   DBNHLM010000090 DBNHLM010000000
VERSION     DBNHLM010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5972)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1330168"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1691
                     /locus_tag="AC16QF_04285"
     CDS             <1..1691
                     /locus_tag="AC16QF_04285"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6447802.1"
                     /translation="RVPRYKADLWIKDGKVARIGVGRKATADRVIDAGDNIVAPGYID
                     IHTHYDAQILWDPYCTISGWHGVTSVVLGNCGFGFAPVPPHLRERAMLMMTRNEAIGF
                     ETMKEGMEWDRYGWETMPEWFEHLKRNVRLGVNATALVPLNPLYAYVMGGIDEAKERR
                     PTAAEKEEMIRLMYEALDHGACGFSYQRCGVPSVQPDWDGSPMITDMLPDAEIIEFGR
                     ALGDYGRGFIEMFDAAPSDHDTVEAFMTSLARASGRPIVRNILLANDEYPKAHRAFLE
                     WLNEAHEEGLQVFGMGFTVRSPTVLTFEDWSLWDGFPGWNRVMNGPKEQRAELMRNES
                     MRVQLRKEMDTGRVGGLGTSGKPAKLIINDTAGYGELDHYLGRMVSEIAETENKHIVD
                     VILDAVLASDFKVEFRGNAYDTSVETTMEVLNSPYVVPGISDGGAHTHFSVQGAYPTD
                     LLEWLVREEGVMTAERAHYGLSRLPAHMCDLGDRGIIREGAPADLVIYNPETIRRTPS
                     WDTLDKLRDLPANEWRLVQRAEGLNYTLVNGVVTFEGLECTGATPGELMLHGEV"
     gene            1695..2240
                     /locus_tag="AC16QF_04290"
     CDS             1695..2240
                     /locus_tag="AC16QF_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6447803.1"
                     /translation="MATDAGHGGAAMEAQADEIGRLREEVARLGARLQHLEAVQAVRE
                     TLLDYAHYHDRGLSGELAELFAEDAVLDISGFGDDLDVSISGRGAIRDMYASLDARSD
                     GPPPYKHAITNLRIEIDGEQALASSYLLDWGGPATDLGPGGSVYRQRLERQSDGRWLI
                     THKRIICTAELTVEAASSLPM"
     gene            2274..3242
                     /locus_tag="AC16QF_04295"
     CDS             2274..3242
                     /locus_tag="AC16QF_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6447804.1"
                     /translation="MAGKLEGRVAVVTGAGRGVGRAVALLFAREGAKVVVADNGSNVD
                     GSGASTGPAHEVVEEIQAAGGIAMADTGDVSDWDQAEALINRPLEAWGALDILVNIAG
                     NFRVDNVTNVTRDNWDALRRVHMDGMMFTSRFAARHWAGGSGYGRLLNMTSDSAMSGV
                     PDTFAYAAAKSAVVGMTRAVANALVSYGVTANCLTQGSLTRMGDSYFGPDAEGRNRSE
                     LATPDQLPEQVAPLVTYLASPAAGHISGRIFGAYGFRYVRWSEPHHERALETEGAWDL
                     DDLFERFPETLGEGLSLERDLLWPMESLDAPSGETLDKGPALGETR"
     gene            3297..4181
                     /locus_tag="AC16QF_04300"
     CDS             3297..4181
                     /locus_tag="AC16QF_04300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6447805.1"
                     /translation="MKFGISMFPTDFSVGPAELARAIEHHGFESFWVSEHSHMPLTTQ
                     FPLADSVPRDYASMLDPFVALAAAAAVTSDILLGTAICLVPQRDPINCAKEVASLDHV
                     SGGRVVFGIGAGWNGPEMRNHGTDPSRRFRVMRERVEAMKALWTSEEAEYHGEMEDFD
                     PVWQWPKPVQRPHPPILVAGAHPNVLKRTVAYGDGWLPVVVPALNEQTRGRMTSLAEL
                     TEWVPRLRALADEAGKPPPIVTTLGMVPDRETLATYERLGIDRVVLGLTSAPMEDALK
                     QLEQHVVSVEAAGGRLGN"
     gene            4321..5847
                     /locus_tag="AC16QF_04305"
     CDS             4321..5847
                     /locus_tag="AC16QF_04305"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016837 - carbon-oxygen lyase activity,
                     acting on polysaccharides [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltF family protein"
                     /protein_id="HIR6447806.1"
                     /translation="MAGKRLLAALLFLWMTACSDDAQVVEEPAPSEEESSAPEDRPVV
                     QADLESVEVDAQPLLPSVLGAELSEARYDLEGLKQRGLLRVLVGYSHTHYFVDGLTIR
                     GISAENLRQFEPFLQKRLGSPRPRIHVLPIPVARDEMVAFLDQGFADMAVGNITITEE
                     RAELVDFSRPLQSDVSELVVHHKDVLNVSSLDDLAGQAVHVRLSSSFVQSVEALNEDL
                     GRKGLEAIRIVAMDEHLQTEDILELVNAGVIPLTVADSHMAKFWAGVHDDIVVRDDLA
                     LRSGRKIAWAIRKDLTGLKPIVDDFIRRHREGTLVGNVLLQRYLDRNRYVQNAAASAD
                     RKRFEEVAELFQRYSKDYDFDWLLLAAQAYQESRLIQSLKSPAGAVGVMQLLPATALA
                     PPVQISNIEDLEANIHAGHKYLRHIADHYFDDPEIDELNLHLLSFAAYNAGPTRISRL
                     RRETARRNLDPNVWFGNVERVVSEKVGREPVTYVRNIFKYYVTYKLMRDQGRSLGEGT
                     "
BASE COUNT         1108 a         1818 c         1990 g         1056 t
ORIGIN      
        1 cgcgggttcc ccgctacaag gccgacctct ggatcaagga cggcaaggtc gccaggatcg
       61 gcgtcggccg caaggccact gcagaccggg tgatcgacgc cggcgacaac atcgttgctc
      121 cgggatacat cgacatccac acccactacg acgcccagat attgtgggat ccgtactgca
      181 cgatctccgg ctggcacggg gtcacctcgg tggttctggg aaactgcgga ttcggcttcg
      241 caccggtccc gccgcacctg cgcgagcgcg ccatgctgat gatgactcgc aacgaagcca
      301 tcggcttcga gaccatgaaa gaggggatgg agtgggacag gtacggctgg gaaaccatgc
      361 cggagtggtt cgagcatctc aagcgcaatg tgcgcctcgg cgtcaacgcc accgccctgg
      421 tgcccctcaa cccgctttac gcctacgtga tgggcggaat cgacgaggcc aaggagcgcc
      481 ggcccacggc ggcagagaag gaggagatga tccgcctcat gtacgaagcc ctcgatcacg
      541 gtgcgtgcgg cttttcgtac cagcgatgcg gggtgccctc ggttcagccc gactgggacg
      601 gttcgccgat gatcacggac atgctgccgg acgccgagat catcgagttc ggcagggcgc
      661 tgggcgatta cggccgcggg ttcatcgaga tgttcgatgc ggcgccctcg gaccacgata
      721 ccgtcgaggc cttcatgacc tccctggccc gcgccagcgg acgtcccatc gttcgcaaca
      781 tcctgcttgc caacgacgag tatccaaagg cgcaccgggc ctttctggaa tggctgaacg
      841 aggcccacga ggaagggctt caggtgttcg gcatgggttt caccgtgcgc tcgcccaccg
      901 tgctgacgtt cgaggactgg agcctctggg acggcttccc tggctggaac agggtgatga
      961 acggccccaa agagcagcgg gccgagctca tgcgtaacga gagcatgcgg gtgcagctcc
     1021 gcaaggagat ggacaccggc cgcgtcggcg ggctcgggac cagcggcaaa ccggcgaagc
     1081 tgatcatcaa cgacacggcc gggtacgggg agctcgatca ctatctgggc cgaatggtgt
     1141 ccgagatcgc ggagaccgag aacaagcaca tcgtcgacgt gatcctcgac gcggtgctgg
     1201 catccgactt caaggtggag tttcgcggca acgcttacga cacctcggtg gagaccacca
     1261 tggaggtact caacagcccc tacgtcgtgc ccggcatctc cgacggcggc gcccacaccc
     1321 acttctccgt gcagggggcg tatcccaccg atctgctgga gtggctcgtg cgagaggagg
     1381 gcgtgatgac cgctgagcgt gcccactacg ggctgagccg cctgccggcg cacatgtgcg
     1441 atttgggcga tcgcggcatc attcgcgagg gcgcgcctgc ggatctggtg atctacaacc
     1501 cggaaaccat tcgccgcacg ccctcctggg acacgctcga caagctgcgc gacctgccgg
     1561 ccaacgagtg gcgcctggtg cagcgcgcgg agggcctgaa ctacaccctc gtgaacggtg
     1621 tggtcacctt cgagggcctg gagtgcacgg gcgcgacccc cggtgagctg atgctgcacg
     1681 gggaggtctg agagttggcg acggacgccg gccacggggg agcggcgatg gaggcgcagg
     1741 cagacgaaat cggtcggctg cgtgaggagg tggcccggct gggcgcgcgg ctgcagcacc
     1801 tggaagcggt tcaagcggtg cgggagacgt tgctggacta cgctcactac cacgatcgag
     1861 gcctttccgg cgagctggca gagctttttg ccgaagatgc cgtgctggac atctccggat
     1921 ttggcgatga tctggacgtc tcgatttccg ggcgtggcgc cattcgtgac atgtacgcga
     1981 gcctggatgc gcgctcggat ggcccgccgc cctacaagca tgcgataacc aacctgcgca
     2041 tcgagatcga cggtgagcag gctttggcgt cgagctacct actggattgg ggtggccccg
     2101 ccaccgatct cggaccggga ggcagcgtct accgccagcg cctcgagcga cagagcgacg
     2161 ggcgctggct catcacacac aaacgcatca tctgcaccgc ggagctcacc gtggaagcag
     2221 cgtcgtcgtt gccaatgtag cgcagcaatc cgccaccaga acgggagact tctatggcag
     2281 gcaagctcga gggcagggtc gcggtggtga ccggtgcggg acgcggcgtc ggtcgcgctg
     2341 tcgcgctgct gttcgctcgc gaaggcgcga aggtggtcgt tgcggacaac ggcagcaacg
     2401 tcgacggtag cggcgcgagc acggggcccg cgcatgaggt ggttgaggag attcaggcgg
     2461 cgggcggcat cgccatggcc gacaccgggg acgtctcgga ctgggatcag gccgaggcgc
     2521 tcatcaacag gcctctcgag gcctggggtg cgctggacat cctcgtgaac atcgccggca
     2581 atttccgtgt cgacaacgtc accaacgtca cgcgcgataa ctgggatgcg ctgcgtagag
     2641 tgcacatgga cggcatgatg ttcaccagcc ggttcgctgc ccggcattgg gccggcggga
     2701 gcggctacgg ccgcctgctg aacatgacgt ctgattcggc catgtcgggc gtgcccgata
     2761 cctttgctta cgccgccgcc aagtcagcgg tggtaggcat gacgcgcgcg gtcgccaacg
     2821 cgctggtgag ctacggggtt acggcaaact gcctgaccca gggttcgctg acgcgcatgg
     2881 gagacagtta cttcggtccc gacgccgagg gtcgcaatcg cagcgagctg gcgactccgg
     2941 atcagcttcc cgagcaggtc gctccccttg taacgtatct tgcttctcca gcagcaggcc
     3001 acatcagcgg tcggatattc ggtgcctacg gcttcaggta cgttcgttgg agcgagcccc
     3061 atcacgaacg ggcattggag accgaaggcg cttgggatct ggacgatctt ttcgaacgct
     3121 ttccggagac tctgggcgaa ggattgagcc tcgaacgcga tctgttgtgg cccatggagt
     3181 cgctcgacgc gccttcgggc gagaccctgg acaaaggtcc ggcgctcggt gagacccgct
     3241 gagtcgccgc taacatttgg tcagtcgaaa catcaggcat tgaggaggat tcggcagtga
     3301 agttcggcat cagcatgttc cccacggatt tcagcgtcgg gccggcggag ctggcgcggg
     3361 ccatagagca tcacggcttc gaatcgttct gggtctccga gcactcgcac atgccgctga
     3421 ccacccagtt tccgctggcc gacagcgtgc cgcgcgacta cgccagcatg ctggacccgt
     3481 tcgtggcgct ggctgccgcg gcggcggtca ccagcgacat cctgctgggc accgcaatct
     3541 gcctcgtccc tcagcgggat cccatcaact gcgccaagga ggttgccagc ctggatcacg
     3601 tctccggcgg acgcgtggtg ttcggcatcg gtgccggatg gaacgggcca gagatgcgca
     3661 accacggcac cgatccgtcc aggcggtttc gcgtgatgcg cgagcgggtg gaagcgatga
     3721 aggcgctctg gacgagcgaa gaagccgagt accatggcga gatggaggac ttcgatccgg
     3781 tctggcagtg gccgaagccc gtccagcgac cgcatccgcc gatcctcgtc gcgggtgcgc
     3841 atccgaacgt gctcaaacga acggttgcct atggcgacgg ctggcttccc gtggtggttc
     3901 cggcgctcaa cgagcagacc cgtggccgca tgacgtcgct cgccgagctg acggaatggg
     3961 tccccagact gcgcgcgctc gccgacgaag cgggcaagcc gccacccatc gttacgaccc
     4021 tgggtatggt ccccgatcgg gagacccttg ccacctacga gcgcctgggg atcgaccggg
     4081 tggtgctggg gctcacctcg gcgccgatgg aggacgctct taagcagctc gaacagcacg
     4141 tcgtcagcgt ggaggcggct ggtgggcgcc tgggcaactg atcgccccga ggagggcggc
     4201 cccgacgagg ttcggatcgt tctttgatca cctccccccg gtggatatcc gtcagccgtc
     4261 gggcaatcat tagcgggcat tctgacttcg ggtgcgacga ctcaggtccg ggagggctcg
     4321 atggctggga agaggctgct ggccgcattg ctgtttctct ggatgacggc ttgcagcgat
     4381 gacgcgcagg tggtggagga gccggcgccg tcagaagagg aatcctcggc acctgaggac
     4441 cgtccggtcg ttcaggccga cctggagtcc gttgaggtcg acgcgcaacc gctgttgccc
     4501 agcgtgctgg gcgcggagtt gagcgaggcc cgctacgatc tggaaggcct gaaacagcgc
     4561 ggtcttctcc gcgtgctggt gggttactcg catacccact atttcgtgga tggcctgacg
     4621 atccgcggta tcagtgccga gaacctcagg caattcgagc cgtttctgca gaaacggctc
     4681 ggatcaccaa gacccaggat ccacgtgctg ccgatacccg tcgctcgaga tgaaatggtc
     4741 gccttcctcg accagggatt cgccgacatg gcggtgggca acatcacgat caccgaagaa
     4801 cgggctgagc tcgtggactt ctcgcgacct ctgcagagtg acgtctcgga gctggtcgtt
     4861 caccacaagg atgtcctgaa cgtctcctcg ctcgacgacc tggccgggca agcggtacat
     4921 gtgcgtcttt cgagcagctt cgtgcagagc gtcgaagcat tgaacgaaga cctcggaagg
     4981 aaaggcctcg aagcaattcg catcgtggcg atggatgagc atctgcagac ggaggacatt
     5041 ctcgagctgg tcaatgcggg agtcataccg ctgaccgtgg cagacagcca catggccaag
     5101 ttctgggccg gcgtccacga cgatatcgta gttcgagacg atctggcgct gcggagtggt
     5161 cggaagatcg cgtgggccat ccgcaaggac ctgaccggat taaaacccat cgtggacgac
     5221 ttcattcgca ggcatcggga aggcacgctg gtcggcaacg tgttgctcca gcggtatctg
     5281 gaccggaaca ggtatgtgca aaatgccgcg gccagcgcgg accgcaaacg ctttgaggaa
     5341 gtggcagaac tctttcagcg ctactcgaag gactacgact tcgactggct gctgcttgca
     5401 gctcaggctt atcaggagtc gcggctgatt cagagtctca agagcccggc tggtgcggtc
     5461 ggagtgatgc aactgctccc cgccacggct ttggcgccgc ccgtccagat ctccaacata
     5521 gaagatctcg aggcgaatat tcacgcggga cacaaatacc tgcgtcacat cgccgatcac
     5581 tacttcgatg atccggagat cgacgagctg aatctccacc tcctctcttt tgccgcctat
     5641 aacgccggcc cgacgcgaat cagccggttg cggcgcgaga cggcaaggcg gaatctggac
     5701 cctaacgtct ggttcggtaa cgtcgagcgc gttgtttcgg aaaaggtggg ccgcgagccg
     5761 gttacctacg tccgtaacat cttcaaatac tacgtgacgt acaagctcat gagggatcag
     5821 ggacgttcgc tgggtgaggg gacgtagatc cccaagttca gcgcagacgt cgatggcggc
     5881 ctgggctgag tctgccttcg cctcagagcc ttcatttcgc aaacatagtt cgagctatcc
     5941 tcgttcggta cgcgtatcat ggcttcctga tt
//
LOCUS       DBNHLM010000091        10083 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1629535, whole genome shotgun sequence.
ACCESSION   DBNHLM010000091 DBNHLM010000000
VERSION     DBNHLM010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10083)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10083)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10083
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1629535"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="AC16QF_04310"
     CDS             complement(<1..623)
                     /locus_tag="AC16QF_04310"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6447807.1"
                     /translation="MNIRSCGGTAAFVLAGLAAGLLESRPVSAAEEESALVHEEVIVT
                     ARRREESLQTVPVAVSVLTGEELVEQGGMKIDAIGKLVPNVHFEAAGGTSGVKSPIVF
                     IRGMGQNDFIPVEDPAVGIYLDGVYMGRNIGSVFDLVDIERVEVLRGPQGTLFGRNTI
                     GGAVNIISKKPGDEFGGSLKVAGGDEGYLEAQGSVNLPLGDRAAARL"
     gene            complement(836..2797)
                     /locus_tag="AC16QF_04315"
     CDS             complement(836..2797)
                     /locus_tag="AC16QF_04315"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR6447808.1"
                     /translation="MRRTLCVALCFAAALLSGCSEQGPESASQAATPAAATEEAEQLQ
                     PGERGAQDYADEIFARESMPGKAVYAQHCAACHDGAVKKAPHKLMLGLMTPEAVLETL
                     STGLMKEQGALLDDEDKRLVSEYLAGVPMGTQAATAVEVCSGAAAEFDFNDPPRTTNW
                     GLQRTNTRSISAADAGLDKASLADLRLKWAFAFPGANRARSQPTLAAGAIYVGSHNGL
                     VYALDRSSGCVRWTYESAAEVRTGIVVDSWNAGDSEAKPRAYFGDVLGNVYGIDAVNG
                     TEIWRMRADEHPNATITGTPSLHDGRLFVPVSSLEVSLAADPAYECCTGRGSVVAVDA
                     ASGEMLWQTYTIEEEPTVQGQNRVETDMYGPSGATVWNSPSIDAKRNQLYVGTGENMS
                     SPATLTSDAIFAMDLETGKVKWVFQATPNDAWNSACDTDRPDSCPVEDGPDFDFGAAT
                     MLLATEKAGDLVIGGQKSGLVHALDPETGKLVWQSRVGRGGIQGGIHFGMASDGKRLY
                     VPISDMRDGRTYPDPERPGLHALDPSTGQELWSFENPDLCTGRDFCHPGISQAITSAG
                     ALVLAGGMDGVMRVHDAATGALLWERDTTETIATLSGARAFGGSFGGGSAPVVRDGLV
                     VLSSGYGIYGHMPGNLLMVLEAEEAPAAD"
     gene            2901..3776
                     /locus_tag="AC16QF_04320"
     CDS             2901..3776
                     /locus_tag="AC16QF_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6447809.1"
                     /translation="MDSVRDKVAFITGAASGIGFGLASVFSREGMRVVMADVEEKALE
                     LAAGALAEGGAEVVPVQLDVTDRDAMARARDRALDAYGAVHLLCNNAGVNAAGPLYRV
                     TYEDWDWVLGVNLGGVVNGVTAFLPELMRHGRDAHVVNTASVGGLVGMPGLGIYNAAK
                     FGVVGLSEALHADLMESGVGVSVLCPGVVRTALTTSDRNRPAHLTSGQATATGESDEP
                     SANEASAMGTEPLDLAEQVLEGVLENRFFLCTHPEFREIVAERNAVLNASFTGTADPA
                     LVEAMRGLVRPIGRG"
     gene            complement(3773..4279)
                     /locus_tag="AC16QF_04325"
     CDS             complement(3773..4279)
                     /locus_tag="AC16QF_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6447810.1"
                     /translation="MEEQLQELLDKKACEEVLLRYGRTQDWLDTPGQASCFWPDAEVD
                     FGFFTGNGEDWVKTVMPHEQEALRRWHMSTSIMIQVDGDRATAECYGIAAGTAEIDGE
                     LMDTVYGGRYMDELKKRGGEWRISKRTYVLDWSHRFPNALEANTGEDFALHVLKIDAP
                     GHPLYREL"
     gene            complement(4316..4768)
                     /locus_tag="AC16QF_04330"
     CDS             complement(4316..4768)
                     /locus_tag="AC16QF_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6447811.1"
                     /translation="MTTIAELLELEEIKNLRHMYSHYFDGKQVDKLADLFTDDAICEF
                     GPDYGGDWVGKEQIRANYATYTEAEGDEHEVMHAVTNPWIRFIDETTANGRWYLLDLR
                     TAEGAENPLILFGIYDDVYKKVDGQWLIHRTRIDFLWPKRHFHGYRDL"
     gene            4918..5745
                     /locus_tag="AC16QF_04335"
     CDS             4918..5745
                     /locus_tag="AC16QF_04335"
                     /inference="COORDINATES: protein motif:HMM:NF018038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR6447812.1"
                     /translation="MEPVRVLLVTKGHAFDHDAFLAMFEGMDELDVTMVEQPAAQVIL
                     RPENVGDYGAVLFYDMSGIPGIGLMHDGAGDTGIPPDDYRRSIEALIDGGTGIVMLNH
                     GTVSWPLWPLWRSISGSSFMLTEGELNGQRVPGSGYRGGHGPYPNPTISLTPVDPSHP
                     VAAGLGNGFTVTDEIYLKTATLGPKVTPLFESDYEFVAANFTPPPLAPPEEQAAWSHP
                     EGSNVVVWANAAGNSPVVAADIGDSPEAFGNADFRRLLRNALQWVASPEARQWVANR"
     gene            complement(5804..6835)
                     /locus_tag="AC16QF_04340"
     CDS             complement(5804..6835)
                     /locus_tag="AC16QF_04340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6447813.1"
                     /translation="MAEHASNEGVNLQLHLKRVPEGLAGPGDFEVRETEIPRPGDGEV
                     LVRAHYFGLDAALRLIVRDSKDFLFRVEPGDLVRGSMAGEVIESNNPDYSVGDLVLAS
                     SGVQNYAISDGSDLERCDTSQAPLGSWLGGFGVSGLTAYFAITEECKPQPGQTVVVNG
                     AAGAVGSIAGQIARLAGARTIGITSSDEKCRWLTEELKYDHAINYKAGNLYDQLAEAA
                     PERIDVIFDNVGGDILDESLRLIGMRGVVLLCGSTSQYAAEHMTGPDNYIWLGTMRAR
                     MQGFVVFDYADRYEEARRRMARWIKDGQLKLPEYIVDGDIGDYPEAFLQLYQGANRGK
                     MLLRLPAAR"
     gene            6916..7149
                     /locus_tag="AC16QF_04345"
     CDS             6916..7149
                     /locus_tag="AC16QF_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447814.1"
                     /translation="MTLADWLIVLGLGGIWHGVQIIWVGGLPRQVRRGETPTAPKGTP
                     EAFGLFWIDQYGYIGLTLALGGAAAVVAGLLGS"
     gene            7146..7529
                     /locus_tag="AC16QF_04350"
     CDS             7146..7529
                     /locus_tag="AC16QF_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HIR6447815.1"
                     /translation="MMPVTLLYAGVMGLMSVFLANLVLRARVRPGQPEWRGDATLRVQ
                     ANFVENVPLAVVLMYLVELSGVGAVAMHAFGVSLVALRVLHVWGLDRAPGANVNYPRL
                     IGAQGTFVLLSVLGVTAVYQFLRAT"
     gene            7539..9092
                     /locus_tag="AC16QF_04355"
     CDS             7539..9092
                     /locus_tag="AC16QF_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6447816.1"
                     /translation="MNIGNITAKWAALDPSRPAIVDIPTGRRVSFGELDERVRQLANG
                     FCAQGFEKGARIGVLARNSIEYFEVYYACARAGLIAQPLNWRLGVDELARILEDGGPS
                     AIVTSAEFRGERDALQGLVDVPRWLEFGASSDGSFEDLVRASSAEEPQRSDSTGDDDP
                     VLILYTGGTTGLSKGALHSHRSLFMGMVNQTVAERVVPTDVYMLTGQMFHIPVGLAMN
                     YMAHGCPVVLMNFEARQALEIIQAERVSAFLGITTMLNWMMAVDDFASYDLSSLRNIQ
                     YGGGPMPGTVVKAALESFPCTLIQGYGQTEGMTMTFLSQEDHADAVAGKHPERLNSCG
                     REGFVTRVQVVNEQGEPVPKDGATAGEIVVRSDANMLGYWGRPDLTAQTIRNGWMWTG
                     DVATWDEDGYVFIVDRAKDMIISGGENIYSTQVEAAIHKHPAVLEAAVIGVPDDEWGE
                     AVKAVVVLKPGETATEQEIIDTARENLASYQKPKSVDFVDSLPKAPTGKILKRELRDP
                     YWKDQDRNV"
     gene            complement(9089..>10083)
                     /locus_tag="AC16QF_04360"
     CDS             complement(9089..>10083)
                     /locus_tag="AC16QF_04360"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6447817.1"
                     /translation="SGKRSIALDLQHEDGKSVLWRLLETADVVMQGFRPGVIGRLGFD
                     SETVRDRLPAIVYCSSSGFGPHGPYAQLPVYDPVIQALSGWAGAQRYDEGPRLVRGMV
                     ADKVAALTSAQAVTAALLQRSMTGRGLHVELSMLEANIAFNWSDVMMHASLLDADATH
                     RPNLLSSYRLFPCRDGWVTVTAGTDAQWRAFSQALERPELAEDARFVTASARAGHVAD
                     WYEAMGEAVAAFGLQEVLDRLRTADVPVAPVLDPTEVAEDPQVLAAGLLREVDHPVAG
                     RLRQPRPSAEIFGSSQDLIPAPTHGQHTAQVLAEIGYDEGAIGRLAADGVVKVD"
BASE COUNT         1657 a         3209 c         3340 g         1877 t
ORIGIN      
        1 ctcagccggg ctgcggcccg atcgcccagg ggcaggttca cgctgccctg ggcttcgagg
       61 taaccctcgt ctccgccggc caccttcaac gagccgccga actcgtcgcc cggcttcttg
      121 gagatgatgt tgaccgcgcc gccgatggtg ttgcggccga acagggtgcc ctgggggccc
      181 ctgagcactt ctacccgctc gatgtctacg aggtcgaata cgctgccgat gttgcggccc
      241 atgtacacgc cgtcgagata gatgcctacg gcaggatctt cgaccgggat gaagtcattc
      301 tggcccatgc cgcggatgaa gacgatgggc gatttgacgc cgctggtccc gccggccgcc
      361 tcgaaatgca cgttgggcac gagcttgccg atggcgtcga tcttcatgcc gccctgctcg
      421 acgagttcct ctcccgtcag caccgaaacg gccacaggca ccgtctgcag gctctcttcg
      481 cgacgccggg cggtgacgat gacttcctca tgcaccagcg cggactcttc ctcggcggct
      541 gagaccggtc gactctcgag cagtccggct gccaggcccg ccaatacgaa tgcagcggtt
      601 ccgccgcaag atctgatatt cataaccccc tccccaggaa gctttgttgt ctatcgcctc
      661 agcaccccgg tgttcgttcg cgagggtgct caccgtcttc catgccaaga ccgggacgcg
      721 caaattcctt cccttggccg gatcgagggt caatgatgtt gactattgag cctgccgtca
      781 accgatggtc cgatccggga agtgccagcc catcgacttc ggtgcccggg ccgggtcagt
      841 cggctgccgg cgcttcctct gcttccagca ccatcaacag attgcccggc atgtgcccgt
      901 agattccgta gccggagctc aggaccacca gcccatccct gaccacaggg gccgagccgc
      961 cgccgaacga tccaccgaag gcgcgggcgc ccgacagggt cgcgatggtt tccgtggtat
     1021 cgcgctccca gagcagagcc cccgtggcgg cgtcgtgaac tctcatgaca ccgtccatgc
     1081 cgccggccag caccagggcg ccggctgacg tgatggcctg agatatgccg ggatggcaga
     1141 aatcgcgccc ggtgcacagg tcggggtttt cgaaggacca caattcttgg cccgtgctcg
     1201 gatccagcgc gtgcaggccg ggcctttccg gatccggata cgttctgccg tctctcatgt
     1261 cgctgatggg cacgtagagc cgcttgccat ccgacgccat gccgaaatga atcccgccct
     1321 gaatgccgcc gcgacccaca cggctctgcc agacgagttt gcccgtctcc gggtccaacg
     1381 cgtggaccag acccgacttc tggccgccga tgacgagatc gccggctttt tccgtggcca
     1441 gcagcatcgt cgcggcgccg aagtcgaagt ccgggccgtc ttccacgggg cagctgtcgg
     1501 gccggtcggt gtcgcatgcc gagttccagg cgtcgttcgg cgtcgcctgg aatacccact
     1561 tcacctttcc ggtttcgagg tccatggcga agatcgcgtc gctggtgagg gtggcaggcg
     1621 aggacatgtt ctctccggtg ccgacgtaga gctgattgcg ctttgcgtcg atggacgggc
     1681 tgttccagac cgtggccccg gacggtccgt acatgtccgt ctcgacccgg ttctggccct
     1741 ggaccgtggg ctcttcctcg atggtgtagg tctgccagag catctcgccg ctggcggcat
     1801 ccaccgccac caccgagccc ctgccggtgc agcactcgta cgccggatcg gccgccaggc
     1861 tgacctccag ggacgaaacc ggaacgaaga gccgtccgtc atgcagcgac ggtgtgccgg
     1921 tgatggtggc gttggggtgc tcgtcggccc gcatgcgcca gatctccgtg ccgttgacgg
     1981 cgtcgatgcc gtagacgttg cccagcacgt cgccgaagta ggctctgggc ttcgcctcgg
     2041 aatcgcctgc gttccagctg tcgaccacga tgccggtgcg tacctcggcg gcggactcgt
     2101 acgtccagcg aacgcagccg ctcgagcgat cgagcgcgta gaccagaccg ttgtggctgc
     2161 ccacatagat cgcgccagcg gccagggtgg gctgggatcg ggcccggttg gcgcctggaa
     2221 acgcgaaggc ccacttgagc ctgaggtcgg caagactggc tttgtcgagg ccggcatccg
     2281 cggcgctgat ggaacgcgtg ttggtgcgct gcagccccca gttggtcgtc ctcgggggat
     2341 cgttgaagtc gaattcggcg gccgcaccgg agcagacttc caccgcggtc gccgcctggg
     2401 ttcccatcgg cacgccggcc aggtactcgg acaccaggcg cttgtcctca tcgtccagaa
     2461 gggcgccttg ttccttcatc aacccggtgc tgagcgtctc cagaaccgcc tcgggcgtca
     2521 tcagcccgag catgagcttg tgaggcgcct tcttgactgc tccgtcgtga caggcagcgc
     2581 agtgctgggc gtaaacggct tttccgggca tggattcgcg ggcgaagatc tcgtcggcat
     2641 aatcctgcgc gccccgctcg ccgggctgga gctgctctgc ctcctccgtg gcggcggccg
     2701 gcgttgccgc ctgagatgct gattcgggcc cctgttccga acaccccgac agcaaggcgg
     2761 ccgcgaagca gagcgcgacg caaagcgtac gtctcacgta agttctctcc ccatggattc
     2821 ccaatcgaat attggtatca cagtggcatg gacaatgata gtttccagcc aggaatgagc
     2881 gtggaagagg gagacaagcg atggactccg ttcgagacaa agtggctttc attacgggtg
     2941 cggcaagcgg catcggcttc gggctcgcca gcgtgttttc gcgggagggg atgcgtgtgg
     3001 tgatggccga cgtggaggag aaggccctcg agcttgctgc gggcgcgctg gcggaaggcg
     3061 gggcggaggt cgtccccgtt cagctggacg tgaccgaccg cgacgccatg gcccgggcgc
     3121 gagatcgtgc tttagacgcc tacggcgccg tgcacctgct ctgcaacaac gccggggtga
     3181 acgcggcggg cccgctctac cgggtgacgt acgaagactg ggattgggtg ctcggcgtca
     3241 atctcggtgg cgtggtgaat ggcgtgactg cctttcttcc ggaactcatg cggcacgggc
     3301 gggacgccca cgtcgtcaac accgcttccg tgggcggcct ggtgggaatg ccgggcctgg
     3361 ggatctacaa cgcggccaag ttcggcgtcg tcggtctgtc agaagcgctc cacgcggacc
     3421 tcatggaaag cggcgtcggg gtctcggtgc tttgcccggg cgtggtccgc accgcgctga
     3481 cgacgagcga ccgcaatcgc ccggctcacc tcaccagcgg ccaggcgacc gcgaccgggg
     3541 agagcgacga gccgagcgcc aacgaggctt ccgccatggg aaccgaaccc ctggacctgg
     3601 cagagcaggt gctcgagggg gttctggaga accgcttttt cctctgcacc catcccgagt
     3661 tccgtgagat cgtcgcggag cgaaacgccg tgctgaatgc gtcgtttacc ggaaccgccg
     3721 accccgcgtt ggtggaagcc atgcgaggtc tggttcgtcc gatcggtcga ggctaaagct
     3781 cccggtacag ggggtggccg ggcgcgtcga tcttcagaac gtgtagcgcg aaatcctcgc
     3841 cggtgttggc ctccagcgcg ttggggaatc gatggctcca gtccaggacg tacgtccgtt
     3901 ttgagatgcg ccattcgccc ccgcgcttct tcagctcgtc catgtaccgg ccgccgtaga
     3961 cggtgtccat gagctcgccg tcgatctccg cggtgccggc agcgatcccg tagcattctg
     4021 cggtggcgcg atcgccgtcg acctggatca tgatgctggt gctcatgtgc catctccgca
     4081 gggcttcctg ctcgtgcggc atcacggtct tcacccagtc ctcgccgttg ccggtgaaga
     4141 agccgaagtc cacctccgcg tcgggccaga agcagcttgc ctgtcccggc gtatccagcc
     4201 agtcctgggt gcgcccgtag cgcagcagca cctcctcgca cgccttcttg tccagcagtt
     4261 cctgaagctg ttcttccatg ctcgcgtcca tcgtttcgtc cttctgtgtt tcagatcaga
     4321 gatcccggta accgtgaaag tggcgcttcg gccagagaaa gtcgatgcgc gttcggtgga
     4381 tgagccactg cccgtccacc ttcttgtaaa catcgtcgta gatgccgaac agaatgagcg
     4441 ggttctcggc accctcggcg gtgcgcagat caagcaggta ccagcggccg ttggccgtcg
     4501 tctcgtcgat gaaacgaatc cacgggttgg tgaccgcgtg catgacctcg tgctcgtccc
     4561 cctctgcctc cgtgtacgtt gcgtagttgg ctcggatctg ctctttgcct acccagtctc
     4621 caccgtagtc gggtccaaat tcgcagatcg catcatccgt gaagagatcg gccagcttgt
     4681 ccacctgttt gccgtcgaag tagtggctgt acatatggcg caggttcttg atttcctcga
     4741 gctcgagcag ctccgcgatc gtcgtcatgg ttccctccca gggcctgtct accttgacgt
     4801 agattggtgc gtatcgcagg cgggcgtcaa gccgttgcgt cgatcccgcg ccggggttag
     4861 tttctggcag ccgcgtccgc agcggggttt cgactaacgg gaagagagga cgaatgaatg
     4921 gagccggtga gggtacttct ggtcacgaaa ggccacgcgt ttgatcacga tgcttttctg
     4981 gcgatgttcg aggggatgga cgaactcgac gtgacgatgg tcgagcagcc ggcggcgcag
     5041 gtcatcctgc gcccggagaa cgtcggggac tacggcgccg tgctcttcta cgacatgtcg
     5101 ggtattcccg gcatcggtct catgcacgac ggggcagggg acacgggcat tccgccggac
     5161 gactaccgac gcagcatcga ggcactgatc gatggcggaa cgggcatcgt gatgctcaat
     5221 cacgggaccg tctcatggcc gctgtggccc ttgtggcgat ccatatcggg gtcttctttc
     5281 atgctgaccg aaggtgagct gaacggccag cgcgtgccgg ggtccgggta ccggggcggt
     5341 catggtccgt atcccaaccc gaccatcagc ctgacgcccg tcgatccttc tcatcccgtg
     5401 gctgcgggcc tgggaaacgg cttcaccgtg acggacgaga tttacctcaa gacggctacg
     5461 cttggtccga aggtgacgcc gctgttcgaa agcgactacg agttcgtcgc ggctaacttc
     5521 acgccgccgc ccctcgcccc gccagaggag caggctgcgt ggtcgcaccc ggaagggagt
     5581 aacgtcgtgg tctgggcaaa cgcggccgga aacagcccgg tggtggcggc cgacatcggt
     5641 gacagcccgg aggctttcgg caacgccgat tttcgtcgtc tgctgcgcaa cgccctgcag
     5701 tgggtggcgt cgccggaagc ccggcagtgg gtggcgaacc ggtaggagat caaaaccgga
     5761 tcgtcgcccg gggcatgttc gggcccggag ttgggggcga aagtcagcgg gcggccggca
     5821 gccgcagaag cattttgccc cggttcgcgc cctgatagag ctgcagaaag gcttccggat
     5881 agtcgccgat gtccccgtcc acgatatatt ccggcagctt cagctggcca tctttgatcc
     5941 agcgcgccat cctgcggcgg gcttcctcgt agcggtccgc gtagtcgaaa accacgaatc
     6001 cctgcattcg cgcccgcatg gtacccagcc atatgtaatt gtccgggccg gtcatgtgct
     6061 cggcggcgta ctgagaggtg gagccacaca ggagcaccac gccccgcatg ccgatcagcc
     6121 gcaggctctc gtcgaggata tcgccgccca cgttgtcgaa gatcacgtcg atccgttcgg
     6181 gtgcagcctc ggccagctgg tcgtagaggt taccggcctt gtagttgatg gcgtgatcgt
     6241 atttcagttc ttcggttagc cagcggcact tctcgtcgct gctcgtgata ccgatggtcc
     6301 gcgcgccggc tagccgcgcg atctgcccgg cgatggagcc aacggcgccg gccgccccgt
     6361 tcacgaccac cgtctggcct ggctggggtt tgcactcttc ggtgatcgcg aagtaagcgg
     6421 tcaggccgga gacgccaaac ccgccaagcc atgatccgag cggcgcctgg ctcgtgtcgc
     6481 atctctccag gtcgctgccg tcgctgatgg cgtagttctg gacgccggag gacgccagga
     6541 ccagatcgcc gacggagtag tcagggttgt tcgactcgat tacttctccg gccatggagc
     6601 cacgcacgag gtcacccggc tcgacccgga acaggaaatc cttgctgtcg cggacaatga
     6661 gtcgcagcgc ggcatcgagc ccgaagtagt gagccctgac cagcacctca ccgtccccgg
     6721 gccggggaat ttcggtttcg cggacttcga agtcccccgg accagcgaga ccctccggga
     6781 cccgcttcag atggagttga agattgacgc cttcgttgct ggcatgctcc gccatggtga
     6841 accctccccg aaaaactgta tcctacttca gttcgggttg ggggtgaaaa gcttggaaaa
     6901 gccgagggga tcgcgatgac gttggctgat tggttgatcg tcctgggact gggcggaatc
     6961 tggcacggcg tgcagatcat ctgggtgggc gggctgccca ggcaggtgcg gcgcggcgag
     7021 acaccgaccg ctcccaaagg tacgcccgag gcgttcgggt tgttctggat cgatcagtac
     7081 gggtacatcg ggctcacgct tgcgctcggc ggcgcggcgg ccgtcgtggc tggacttctc
     7141 ggctcatgat gcccgtcacg ctgctttatg ccggggtgat ggggctgatg agcgtgtttc
     7201 tcgccaacct ggtgcttcgt gcccgtgttc gaccgggaca gccggagtgg cggggcgacg
     7261 ccacgcttcg cgtgcaggcc aacttcgtcg agaacgttcc gctggctgtg gtgctgatgt
     7321 acctcgtcga gctgagcggc gtcggcgcgg tcgccatgca cgcgtttggc gtctcgctgg
     7381 tggctctgcg ggttctgcac gtctggggcc tggaccgggc tcccggtgcc aacgtcaact
     7441 acccccggct cattggagct cagggcactt tcgtgctcct gtccgttttg ggcgttacgg
     7501 ccgtttatca gttcctgcgt gcaacctagg aggagtacgt gaacatcggc aacatcacag
     7561 ccaagtgggc cgcgctggac ccgtctcgcc cagcgatcgt cgacatcccc acggggcgcc
     7621 gggtctcgtt tggcgagctc gatgagcggg tgcgccagct cgccaacggg ttctgtgccc
     7681 aggggttcga aaagggcgcg cgaattggcg tgctggccag gaacagcatc gagtacttcg
     7741 aggtttatta cgcctgcgcg cgtgcgggat tgattgccca gccgctcaac tggcgcctgg
     7801 gggtggacga gctggctcgc atcctcgagg acggcggccc aagcgccatc gtcacgtccg
     7861 ccgagttccg cggcgagcgg gatgcgctgc aggggctggt ggacgtgccg cggtggctcg
     7921 agttcggcgc ttcgagcgac gggagcttcg aggatctcgt gcgcgcgtcg agcgccgagg
     7981 agccgcagcg gtcggactcg accggggacg acgacccggt attgatcctg tacacgggag
     8041 ggaccacggg gctgtccaaa ggagccctgc actcgcacag atcgctcttc atgggcatgg
     8101 ttaaccagac ggtggccgag cgcgtggtgc ctaccgacgt atacatgttg acgggccaga
     8161 tgtttcatat cccggtgggg ctggcgatga actacatggc ccacgggtgc cctgtggtgc
     8221 tgatgaactt cgaggccagg caagcgctgg aaatcattca ggcagaacgg gtgtccgcct
     8281 ttctcggcat caccaccatg ctcaactgga tgatggcggt ggacgatttc gcgagctacg
     8341 acctttcgag cctccgcaac atacagtacg ggggcgggcc gatgccgggt acggtcgtga
     8401 aggccgcgct cgagtccttc ccctgcacgc tgatacaggg atacgggcag acagagggga
     8461 tgaccatgac gttcctttcc caggaggacc atgccgacgc ggttgccggc aagcaccctg
     8521 agcgcctcaa ttcctgcggt cgcgagggct tcgtgacgcg ggtccaggtg gtgaacgagc
     8581 agggcgagcc ggtcccgaaa gacggcgcca cggccgggga gatcgtggtg cgctcggacg
     8641 ccaacatgct cggctactgg ggacgcccgg atctcaccgc ccagaccatc cgcaacggat
     8701 ggatgtggac gggcgacgtc gccacctggg acgaggatgg atacgtcttc atcgtcgacc
     8761 gcgccaagga catgatcatc tccggagggg agaacattta ctcgacccag gtcgaagcgg
     8821 ccattcacaa acatccggcc gtgctggagg cggcggtgat cggtgttccc gacgacgagt
     8881 ggggggaagc cgtgaaggcg gtggtggtgc tcaaacccgg agagaccgct accgagcagg
     8941 agatcatcga cacggcccgg gagaacctgg cctcttacca gaaaccgaaa tccgtcgact
     9001 tcgtcgacag cctgccgaag gctcccacgg gcaagattct caagcgtgag ctgcgggatc
     9061 cctactggaa ggaccaggac cggaacgtct agtcgacctt gacgacgcca tcggccgcca
     9121 gcctgccgat ggcgccctcg tcgtagccga tctcggccaa cacctgtgcc gtatgctgcc
     9181 cgtgggtcgg cgcggggatc aggtcctgag agctcccgaa aatttcggcg ctcggtcggg
     9241 gctggcgcag ccttcctgca accggatgat cgacctcgcg cagcagcccg gccgccagca
     9301 cctgtggatc ctcggcgact tcggtcggat cgagcacggg tgccacgggc acgtcggccg
     9361 ttctcaggcg gtcgagcact tcctggagcc cgaaggcggc cacggcctcg cccatggcct
     9421 cgtaccagtc cgccacgtgc ccggcacgcg ccgacgcggt gacgaaccgg gcgtcctccg
     9481 ccagctccgg tctttccagc gcctggctga aggcccgcca ctgggcgtca gtgccagcgg
     9541 tcaccgtgac ccatccgtcg cgacagggga acagccgata ggaggaaagc aggttgggcc
     9601 tgtgggtcgc atccgcatcg aggagggaag cgtgcatcat gacgtcggac cagttgaagg
     9661 cgatgttggc ctccagcatg ctgagctcga cgtgtagccc gcgacccgtc atcgacctct
     9721 gcagcagagc cgcggtcacg gcctgggcgg acgtcagagc ggccaccttg tcggccacca
     9781 tgccgcgcac caggcgcggc ccctcgtcgt agcgctgtgc tccggcccag ccggacagcg
     9841 cctgaatgac ggggtcatag accgggagct gggcgtaggg gccgtggggg ccgaagcccg
     9901 atgacgagca gtagacgatg gcaggtaggc ggtctctgac cgtttcgctg tcgaagccga
     9961 gtcttccgat cacgccaggc cgaaaaccct gcatcaccac gtcggcggtc tccaggagtc
    10021 tccagagcac gctcttgccg tcctcatgct gaaggtccag ggcgatcgag cgcttgccgg
    10081 agt
//
LOCUS       DBNHLM010000092         5510 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1390275, whole genome shotgun sequence.
ACCESSION   DBNHLM010000092 DBNHLM010000000
VERSION     DBNHLM010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1390275"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="AC16QF_04365"
     CDS             <1..146
                     /locus_tag="AC16QF_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447818.1"
                     /translation="RVPDELAHSSLRFTVGRYTTEEEVDFAAQRVAEVVAALRGEQSA
                     ASQ"
     gene            251..676
                     /gene="ndk"
                     /locus_tag="AC16QF_04370"
     CDS             251..676
                     /gene="ndk"
                     /locus_tag="AC16QF_04370"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIR6447819.1"
                     /translation="MAVERTLSIVKPDAVARDLIGEIYTRFEKNGLKVVAAKMIRMTQ
                     AQAQGFYAEHDGKPFFADLVAYMTSGPVMVQVLEGDDAVAKNRRLMGATNPKEAEPGT
                     IRADFAESLEANAVHGSDSTTSAAREVAYFFSDEEICVR"
     gene            685..1785
                     /gene="rlmN"
                     /locus_tag="AC16QF_04375"
     CDS             685..1785
                     /gene="rlmN"
                     /locus_tag="AC16QF_04375"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR6447820.1"
                     /translation="MEAGLNNLFGYSRAQLEAFVGELDGRPYRARQIMKWAYHQGVVD
                     FSAMTDLSKSFRAALAERSGFWAPEVVARRQSRDGTVKWLVRAENGNCVETVLIPDRG
                     RYTLCVSSQVGCMLDCSFCSTGKQGFNGNLSAADIVGQVWLADRELAQAGQSVTNVVL
                     MGMGEPLLNFDAVVAATSLMMDDLAFGISKRRVTVSTAGVVPGIYELAKVSDVSLAIS
                     LHAPNDELRDVLVPINRKYPIAELLDACRHYLSGLGERRSLTVEYTLLKGVNDQMTHA
                     RQLAMLLQGLKCKINLIPFNPFPASGYERPDARAVRAFQTHLMNSGYATMLRTTRGED
                     INAACGQLVGQVSDRTKRQERYLARVRAQEVA"
     gene            1782..2531
                     /gene="pilW"
                     /locus_tag="AC16QF_04380"
     CDS             1782..2531
                     /gene="pilW"
                     /locus_tag="AC16QF_04380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HIR6447821.1"
                     /translation="MRLGALAILWSVLLSGCVTETTGGLPEPAVAEKRVQAQLDLARG
                     YLENRDYSRARAPLNRALEIDPRSVEGHVLMAVLLQGEGEMALAEQHYKLALRHEPND
                     SQALNNYASFLYRQQRYDEALGLLRELVKDTEYPARSQAYENLGLAELIAGDREAARQ
                     AFIRALNLNFAQPRSSLELARLSYSDGNFELAHEYYEAFRVRARQSPRSLCLGMKISE
                     ALGNSDQTASYGMALQNLYPESAEADECEVP"
     gene            2557..3540
                     /locus_tag="AC16QF_04385"
     CDS             2557..3540
                     /locus_tag="AC16QF_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024855.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HIR6447822.1"
                     /translation="MSEEEREPKGPGATLREARERMQISTRQVAEALNLPVHTIEAIE
                     AEDPERLPAPVFARGYTRAYARLLELDADAIVAQYPQGEDNVEQVHLIREPRSSASVI
                     RFGLAALLWILGLGVVAVVVWVVWQWSGKDTELAEPVTEARGLLEEVADQPVPSDVGT
                     DPSFSGVPQQEDAALTGREAAEIAGFSAEPEPEPESVAQREVITPRRPGTRPETEPTP
                     SDAGAMATQAAPVEDLAALREAGRRITSAGDDVLRMEFFEDCWVEVMSSTGSLLYGDL
                     NRSGRVLVLVGEAPFRVLLGYAPGVRMSFNGEAVVLTPHTRNAVADLVLGQ"
     gene            3543..4811
                     /gene="hisS"
                     /locus_tag="AC16QF_04390"
     CDS             3543..4811
                     /gene="hisS"
                     /locus_tag="AC16QF_04390"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR6447823.1"
                     /translation="MVQSIRGMRDVLPEEGRRWRVVEDAIRAVLTTHAYEEVHLPLLE
                     ATELFSRGVGEATDIVEKEMYTLADRDGDSISLRPEGTAVCARALLQHGLLHNQTQRV
                     FYAGPMFRYERPQKGRYRQFYQIGAEAFGLAGPDVDAELILMCQLMWKALGISGETRL
                     EINTLGSGAARAAYRAALVGFLTPLAAGLDADSQRRLGSNPLRILDSKVEQTQDLLAG
                     APSLEDFIDDEGKAHFSGLCELLEQLDVPFEINPKLVRGLDYYTHTVFEWKTDALGAQ
                     DTICAGGRYDGLVELLGGRPTPGAGFALGVERVLLLHEQRFQGAGFAAAADVYCCVLD
                     DRRQSEVFAIAQKLRDALPGLRIRTHAGGGKLKSQMRRADASGARLAVLVGEDEIAKG
                     VVSLKYLRESREQESVPAADLETVLAEYFA"
     gene            4843..>5510
                     /locus_tag="AC16QF_04395"
     CDS             4843..>5510
                     /locus_tag="AC16QF_04395"
                     /inference="COORDINATES: protein motif:HMM:NF021492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0044877 - protein-containing complex
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HIR6447824.1"
                     /translation="MSDYLTDEEQVAKLKSWWDENGVSVIVTLVLVVAGVVGWRWYES
                     YHETRVARVSDLYEEFLVSEGDARDASAASIAEEAPDSAYQTLVMLRRSQESMESGDY
                     EAAEAFLVEAVEAAPEPVLADVARVRLARVQQQLDRLDDALETLSGVLGEGYRSQVAE
                     MKGDIHLARGERALAYEAYASALAAVGDNQSRPVLEMKAADTADAVTGAQPAAAPEAG
                     QGLES"
BASE COUNT          987 a         1672 c         1871 g          980 t
ORIGIN      
        1 tgcgggtgcc agacgaactc gcccactcct cgctgcgatt cacggttggc cggtatacga
       61 ccgaagagga agtggacttc gccgcccagc gggtcgccga ggtggtggct gccttgaggg
      121 gcgaacaatc cgcggcgtcg cagtagtaac tgcgcagggg gcgcgtataa tacgcgcccg
      181 ctgaggcgct tcatttcttc gttttttcaa ttacctaggc gcttttcgaa gattctaagg
      241 ggactccttg atggcagttg agcgtacttt atccatcgtc aagcccgacg cggtggcgcg
      301 agatctcatt ggcgagatct acacccgctt cgaaaagaac gggctgaaag tggtggcggc
      361 caagatgatc cgcatgacgc aggcgcaggc tcagggcttt tatgccgagc acgatggcaa
      421 gccgtttttc gccgacctcg tcgcctacat gacctccggc cccgtaatgg tccaggtgct
      481 tgaaggtgac gatgccgtgg cgaagaatcg ccggctcatg ggcgcgacga acccaaagga
      541 ggcggaaccc ggcactatcc gcgcggattt cgccgagtcc ctggaagcca acgccgtcca
      601 cggatcggat tcgaccacct ccgcagcccg tgaggtggca tacttcttca gcgacgagga
      661 aatctgcgtg agatagcgct ggacatggaa gcggggctga acaacctttt cggatacagt
      721 cgcgcccaac tcgaggcctt cgtgggagag ttggacggca ggccttaccg cgcccgtcag
      781 ataatgaagt gggcctatca tcagggcgtg gtcgactttt cagccatgac cgacctgtcg
      841 aagtcgtttc gcgcggcgct tgccgaacgc agcggcttct gggcgcccga ggtggttgcc
      901 aggcggcaat cccgtgacgg caccgtcaag tggctggttc gtgccgagaa cggcaactgc
      961 gtggagacgg ttctgattcc ggaccgcggc cgctacacgc tgtgcgtgtc gtcccaggtc
     1021 ggctgcatgc tcgactgcag cttctgctct acgggtaagc agggtttcaa cgggaatctg
     1081 tccgcggccg acatcgtcgg acaggtctgg cttgcagacc gcgagctcgc tcaggccggg
     1141 caaagcgtca ccaacgtggt gctgatgggg atgggcgagc cactgctcaa cttcgacgcc
     1201 gtggtcgccg ccaccagtct gatgatggac gacctcgctt tcggaatatc caagcgccgg
     1261 gtgacggtca gcacggccgg ggtcgttccc ggcatctacg agctcgcgaa ggtctcggat
     1321 gtgtccctgg cgatctctct gcacgcgccg aacgacgagc tgcgcgacgt gctcgtgccg
     1381 atcaaccgga aatatcccat tgcggagctg ctcgacgcct gtcgccacta tctcagcggg
     1441 ttgggcgagc ggcgctctct gacagtggag tacacgctgc tcaagggggt caacgatcag
     1501 atgacccacg cccgccagct cgctatgctg ctccaagggc tgaagtgcaa gatcaatctg
     1561 attccattca acccgtttcc cgcttcggga tatgaacgtc ccgacgctcg cgccgtgcgg
     1621 gcatttcaga ctcatctgat gaattcgggc tatgcgacga tgctcaggac cactcgcggc
     1681 gaggatatca acgccgcgtg cggacagctg gttggtcagg tcagcgaccg gaccaaacgt
     1741 caggagcgtt atctggcccg cgtgcgtgca caggaggtgg catgagacta ggggcgttgg
     1801 cgatcttgtg gtcggtgctg ctcagcggct gcgtgacgga aaccacgggt gggctgccag
     1861 aaccggcggt tgccgaaaaa cgcgttcagg cgcagctcga cctggctcgt ggctacctgg
     1921 aaaaccgcga ttacagccga gcccgggcac cgctgaatcg cgcgctggag atcgacccgc
     1981 gatcggtgga aggtcatgtc ctgatggccg tgctcttgca gggtgagggc gagatggcgc
     2041 tggccgagca gcattataag ctcgctctgc gccacgagcc gaacgactct caggccctga
     2101 acaactatgc gagctttctc taccgtcagc agcgttacga tgaagcgctc gggctgcttc
     2161 gcgagctcgt caaagacaca gagtatccgg cgcgctctca ggcctacgaa aacctgggcc
     2221 tcgccgagtt gatagccgga gaccgtgagg cggcgcggca ggcgttcatc cgcgcgctga
     2281 acctgaattt cgctcagccc cgctcgagcc tggagcttgc ccggttgtcc tattccgatg
     2341 ggaacttcga gcttgcccac gaatactacg aggccttccg ggtgcgtgcc cgccagtcgc
     2401 cgcgcagcct gtgtctgggc atgaagatca gcgaggcgct aggcaacagc gaccagacgg
     2461 cgagctatgg gatggccctg cagaacctct atcccgaatc tgccgaagcg gacgagtgcg
     2521 aggtgccctg atgatgggcc gagccggggt gacgacttga gcgaggaaga gcgcgagccg
     2581 aaggggcccg gcgcgacgct tcgcgaagcc cgcgagcgca tgcagatctc cacccggcag
     2641 gtggcggaag ccctgaacct gcccgtacac accatcgagg cgatcgaggc cgaagatccg
     2701 gagcgattgc cggcgccggt ctttgcccgc ggctacacgc gtgcctacgc gcggcttctc
     2761 gagctcgatg cggacgccat cgtggcgcag tatccccagg gcgaagacaa cgtcgagcag
     2821 gttcacttga ttcgcgagcc tcggagcagc gcatcggtaa tccgattcgg cctcgcagcg
     2881 ctgctctgga ttctgggact gggcgtcgtc gcggtcgtcg tctgggtcgt ctggcagtgg
     2941 agcggtaaag atacggagct tgcggaaccc gtgaccgaag cgaggggcct gctcgaagag
     3001 gtcgcggacc agccggtacc gtccgacgtc ggcaccgacc cctcattctc aggcgtcccg
     3061 cagcaggaag acgccgctct tacgggcagg gaagcggcgg aaatcgccgg gttttcagcg
     3121 gagcccgaac cggagccgga gtctgtggcg cagcgagagg tgatcacgcc gcggcggccg
     3181 gggacgaggc cggaaaccga accgacgccg tcagatgccg gcgccatggc gactcaggcg
     3241 gccccggtcg aagacctggc tgcgctccgg gaggctggtc ggcggatcac gtccgcaggt
     3301 gacgacgtgc ttcgcatgga gtttttcgag gattgctggg tggaagtcat gtcttccact
     3361 ggaagtctcc tctacggcga tctcaatcga tccggacgcg tcctcgtgct ggtgggagaa
     3421 gcgcctttcc gcgtgctgct cgggtacgcg cccggcgtgc gcatgagttt caacggcgag
     3481 gccgtggtgc tcacgcccca cacccgcaac gccgttgcgg acctcgtgct tggtcagtag
     3541 cgatggtcca gtcgatcaga ggcatgcgcg acgtgctgcc cgaagagggc aggcgctggc
     3601 gggtcgtgga agacgccatc cgcgcggtgt tgaccacgca cgcctacgaa gaggttcacc
     3661 tgcccctgct cgaggccacg gagctgttca gccgtggcgt gggcgaggcc acagacatcg
     3721 tcgagaagga gatgtacacc cttgccgacc gggacggaga cagcatctct ctgcgccccg
     3781 agggcacggc cgtctgcgcc cgcgcgttgc tccagcacgg tctgctccac aaccagaccc
     3841 agcgcgtttt ctatgcaggc cccatgtttc gctacgaacg tccgcagaag ggccgctatc
     3901 ggcagttcta ccagattggc gccgaggctt tcgggttggc ggggccggac gtggatgcgg
     3961 agctgatctt gatgtgccag ctcatgtgga aggctctggg aatctccggc gaaacccgcc
     4021 tggagatcaa taccctcgga tcgggggctg cgcgggccgc ttatcgggca gctctggtgg
     4081 gctttctaac gccgctggcg gccggtctgg atgctgacag ccagcgccgg ctcggctcga
     4141 acccgcttcg cattctggac agcaaggtcg agcagaccca ggacctgctg gccggggcgc
     4201 cctccctgga ggacttcatc gacgacgaag gaaaggcgca cttctccggt ctgtgcgagc
     4261 tgctcgagca gctcgatgtt ccgtttgaaa tcaatccgaa gctggtccgt ggcctggatt
     4321 actacaccca caccgtgttc gagtggaaaa ccgacgcgct cggagcccag gacacgatct
     4381 gtgcgggtgg ccgttacgat gggctggtcg agctgctcgg cgggcggccg acgccgggcg
     4441 cgggctttgc cctgggggtg gagcgcgttc tgctccttca cgagcagcgg ttccaggggg
     4501 ccggcttcgc ggctgcggcc gacgtttact gctgcgtgct ggacgaccgg cgccaatcgg
     4561 aggtattcgc catcgcgcag aaattgcgtg atgcgctccc aggtctcaga atccgcaccc
     4621 acgccggtgg cggcaagctg aagagccaga tgcgacgcgc cgatgccagc ggcgcgcgcc
     4681 tcgcggttct cgtcggcgag gacgagatcg ccaagggcgt ggtgtcgctg aaataccttc
     4741 gcgagagccg ggagcaggaa agcgtcccgg ccgctgatct ggaaacggtg ctggccgagt
     4801 acttcgcctg aacctggcga gcgaatcgaa aaggagattg gggtgtcgga ttacctgacc
     4861 gacgaagagc aggtcgcaaa gctcaaatca tggtgggacg agaacggcgt gtccgtcatc
     4921 gtgacgctcg ttctggtcgt cgccggggtg gtcggttggc gatggtacga aagctaccat
     4981 gagacgcggg tcgcgcgagt ttcggatctc tacgaggagt ttctggtcag cgaaggggac
     5041 gccagggacg caagcgccgc gtccatcgcc gaagaggcac ccgacagtgc ctatcagacg
     5101 ctcgtcatgc tgcgccgctc gcaagagtcc atggagagcg gcgactacga agccgccgag
     5161 gcgttcctcg tcgaggcggt ggaagctgct ccggaacccg ttctggcgga cgttgcccgg
     5221 gtgcggctcg cgcgggtgca gcagcagctg gaccgtctcg atgatgcgct cgaaacgctt
     5281 tccggggtgc ttggcgaagg atatcggtca caggttgccg agatgaaggg ggatatccat
     5341 ctggcccgcg gggagcgcgc ccttgcttac gaagcatacg cttcggcgct tgcggcggtg
     5401 ggtgacaacc agtcccgtcc ggtgctggag atgaaagccg ccgataccgc cgatgccgtg
     5461 accggagcgc agcccgctgc agcgccggaa gccggacagg gcctcgaatc
//
LOCUS       DBNHLM010000093         6189 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1091232, whole genome shotgun sequence.
ACCESSION   DBNHLM010000093 DBNHLM010000000
VERSION     DBNHLM010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1091232"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /gene="aceE"
                     /locus_tag="AC16QF_04400"
     CDS             complement(<1..380)
                     /gene="aceE"
                     /locus_tag="AC16QF_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702974.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HIR6447825.1"
                     /translation="MAYPRDLRDEDAAETREWLDSLEYVLENAGVDRANFLLERLSAR
                     MTRTGARLPYTITTPYRNTIPSSREAVMPGDLFMERRIRSLIRWNAMAMVVQANRIST
                     ELGGHIASFASAATLFDVGFNHFFR"
     gene            complement(415..1389)
                     /locus_tag="AC16QF_04405"
     CDS             complement(415..1389)
                     /locus_tag="AC16QF_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR6447826.1"
                     /translation="MKRIGIDLGGTKIKGIVLAEDGRVLSTRRAATPSQSYAGTVAAI
                     VRMVAELDGDSGEARDLPVGIGTPGIWMAQRSAMKNCNSTWLNGQPLLLDLEHALGAR
                     VRIANDADCFALSEAMAGTGRGYGSVFGVILGTGVGGGFVTGGRLMSGPNGLAGEWGH
                     TPLPYLGLHEALEISVGSELDRRHEQANALESRLAVRTCYCGRPGCVETYLSGPGWRQ
                     THRELWSEDVPTETIAKRRDERSEQTFFLYTRMLARSLAQIVNIVDPAVIVLGGGLSN
                     VQALYQPVNEMLARYVFSGECDTQVKAPQLGAESGVLGAAQLWSDNGG"
     gene            complement(1386..1937)
                     /gene="ampD"
                     /locus_tag="AC16QF_04410"
     CDS             complement(1386..1937)
                     /gene="ampD"
                     /locus_tag="AC16QF_04410"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HIR6447827.1"
                     /translation="MRVRKDHCLVGVPFRASPNCDERPDPEDVSLIVIHGISLPPGRF
                     GGDLVDGLFTNSLPDELDPGLAELRDVRVSAHLLIDRAGRITQYVPFHRRAWHAGSSG
                     WCGRPSCNDYAIGIELEGTDELDYADAQYRALIAVSRALFDRYPRLSVDAVVGHQEIA
                     PERKTDPGPAFDWCRYLGALAGP"
     gene            2024..2881
                     /gene="nadC"
                     /locus_tag="AC16QF_04415"
     CDS             2024..2881
                     /gene="nadC"
                     /locus_tag="AC16QF_04415"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR6447828.1"
                     /translation="MAPDGFPHADELADAVGESVRAALAEDVGTGDITAELIAPDARA
                     TAEVITRDEGIFCGAPWVLETCAQVDAAIEVTLHCADGDRVSPGQTLFALSGPARGLL
                     TAERTMLNFVQLLSGTATATRRYVDLIAGTAARLLDTRKTVPGLRMAQKYAVHCGGGH
                     NHRIGLFDAFLIKENHLAAAGGIAPAVARARELYPDRPVEVEVESLDELDQALAGGAD
                     IALIDNFSLEDTSAAVTLAAGRIELEASGGIDEATITEIARAGVDYISLGVITKRVIP
                     LDLSMRFTS"
     gene            2887..3624
                     /locus_tag="AC16QF_04420"
     CDS             2887..3624
                     /locus_tag="AC16QF_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447829.1"
                     /translation="MTRISAFLIHLGISFAIFLGLAYLVVFIWYPDFFFSTDGGWQGI
                     RIIVAVDLVLGPALTLIVFKPGKPGLKFDLTLIGLFQAACLIAGVYVVHSERPLAIVY
                     VDGQFFSMSGDDYSGVGLPIPDLRAVPGKSPKWVTVEIPHDPWISQEIRMSAMRKGTP
                     LRTLADRYAPFSVEDVDLEREAYPLSELVDRDQLTQQIPLWLDEHGGTLEDYAFVRLA
                     TRYRYVFLGIDRRDGAIAGVLGTPAPL"
     gene            complement(3631..5493)
                     /locus_tag="AC16QF_04425"
     CDS             complement(3631..5493)
                     /locus_tag="AC16QF_04425"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR6447830.1"
                     /translation="MFYGSDVNVVVFFGAIFVLMSGLSLVAPRLATEVHASPVTFALP
                     ALAIVGIVVSYQFSLSKDSSFVPAWVVAVTPLAFLLARGAGEAAFALHKAVSLTVLAF
                     AAISVVLLFSAGVRARLPLTDPNNYATLLCLILLPWLHRYFVLRTGSQGMSLRVEGKD
                     LSACFILALALFATGSRAGLLVIAGAFIIWAGLAIARRLRWRPVAAAAALTAAAYLLA
                     GASLTDQSVALTAQAAAADRVAGADIEVRMALIGSALAMYAEYPATGIGLFVFPLLYR
                     FQRPVVDQDSAGLYVHNDYLQLLLEGGPLLFCAMVTFAVVVVIVFFRRLAALPGDEAF
                     ARLGLTMGVGAALAHALVNFVVYSPALGFVLGLTVAASFRDSKGSGSVDSTQARPIRV
                     GVTLAVLFGWTAWLYLGLDTATAGVLQNQRYVPFTEEVRKSPERMLRFAGMARSLNAD
                     RGVPLLAEAVLLDNKLRQNPESRYLGEETLNAFRRAGRADPWNTLTLLLMADFIMRNP
                     SVLPLLENGEAPEELLLRTLVMDPQFVPGIDALMDKLGDEGRHAEAEALLRLRIYPWL
                     RLLARQNKPAAARYAEALLGMARARGDSEFVSELEARQQEVENTQPVIAQRWFF"
     gene            complement(5601..5676)
                     /locus_tag="AC16QF_04430"
     tRNA            complement(5601..5676)
                     /locus_tag="AC16QF_04430"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5641..5643),aa:Thr,seq:cgt)
     gene            complement(5755..>6189)
                     /locus_tag="AC16QF_04435"
     CDS             complement(5755..>6189)
                     /locus_tag="AC16QF_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007160926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR6447831.1"
                     /translation="GSLVLLPAGTGKPDQGSMAMIKARTSHGFSLIELMIAVAIIAII
                     AAVALPLYQGYIQTSREGVLVNNISTIEIFQEDRMLRTGSYLLTAANTAAITAGIGWT
                     PRDDGELSYSIADGGGGSYDVTATDDTGVTVCMRFPQKVRCP"
BASE COUNT         1207 a         2030 c         1932 g         1020 t
ORIGIN      
        1 cgaaagaaat ggttgaaacc gacgtcgaac agcgtggcgg ccgaggcaaa actcgctata
       61 tgcccgccga gttcggtcga gatacgattg gcctgaacca ccatggccat agcgttccag
      121 cggatcagcg agcggatgcg ccgttccatg aagagatcgc ccggcatcac ggcttcgcgg
      181 ctcgagggaa tggtgttccg gtaaggcgtg gtgatggtgt agggcaaacg cgcgccggtg
      241 cgcgtcatcc gggcagacaa acgctccagc agaaagttgg cacggtccac gcccgcgttc
      301 tcgagaacgt actcgagaga atccagccac tcccgggtct ccgccgcgtc ctcgtcgcga
      361 agatctcttg gatacgccat atcagttccc gctggccggc tagccggccg gacctcaacc
      421 accgttatcg ctccacagct gtgcagcccc gagcaccccg ctctccgcgc ccagctgagg
      481 cgctttcacc tgcgtgtcgc actcgcccga aaagacgtag cgcgcgagca tctcgttgac
      541 cggctgatac agcgcctgca cgttcgacaa cccaccgccc agcacgatca ccgccgggtc
      601 aacgatgttc acgatctgag ccaggctgcg cgcgagcatt cgggtgtaca ggaaaaacgt
      661 ctgctccgag cgctcgtcgc gacgcttggc aatcgtctcc gtgggcacgt cctcgctcca
      721 aagctcgcgg tgcgtctgcc gccacccggg gccgctgagg taggtttcca cgcagcccgg
      781 ccgtccgcag tagcaggttc tgaccgccag ccgagattct agcgcattcg cctgctcgtg
      841 ccggcgatcc agttcgcttc ccaccgagat ttccagcgct tcgtgcaggc ccagataagg
      901 cagcggcgtg tgcccccact cgccggccag cccattcggc ccgctcatca gccgtccccc
      961 ggtcacgaag ccaccaccca cgccggtgcc caggatgacg ccgaagacgc tcccatatcc
     1021 ccggcccgtt ccggccatgg cttccgagag agcaaaacag tcggcgtcgt tggcgatgcg
     1081 tacgcgggcc ccgagcgcat gctccagatc gagcagcagc ggttgcccgt tgagccaggt
     1141 ggagttgcag ttcttcatcg cgctccgctg cgccatccag atgccggggg tgccgatgcc
     1201 cacgggcaga tcacgcgcct cgccggagtc gccgtcgagc tcggcaacca tgcgcacgat
     1261 ggccgcaacg gtgccggcgt agctttgcga cggcgtcgcc gcccgtcgcg tcgacagcac
     1321 ccggccgtcc tccgccagca cgatgccctt gatcttggtg ccgcccaggt cgatgccgat
     1381 gcgtttcacg ggcccgccag cgcgccgagg taacggcacc agtcgaaggc ggggcccgga
     1441 tccgtcttgc gctccggcgc gatttcctgg tgcccaacaa cggcgtccac ggacagccgc
     1501 gggtagcgat cgaacagggc ccggctcacc gcaataagcg cccggtactg cgcgtcggca
     1561 tagtccagct catcggtccc ttcgagctcg atgccgatgg cgtagtcgtt gcacgagggc
     1621 cggccgcacc aaccggatga tcccgcgtgc catgcgcgcc ggtggaaggg aacgtactgg
     1681 gtgattcggc cggcgcgatc gatgagcagg tgggccgaaa cgcgcacgtc acgaagctct
     1741 gccagcccgg ggtccagctc atcgggcagg gagttggtga agaggccgtc gacgagatcg
     1801 ccgccgaaac gacccggcgg cagggatatc ccgtggatca cgatcagcga gacgtcctcg
     1861 ggatcgggac gctcgtcgca gttgggcgag gcccggaacg gaaccccgac gaggcaatgg
     1921 tctttcctga cgcgcaaaac ctgtgatccg tgaccggcac tgaagcagat tctattgaag
     1981 tagaatggcc cgcgcacaac tatcggatat cggaggcagc ccgatggcgc ccgacgggtt
     2041 cccacacgcc gacgagctgg cagacgccgt gggcgaaagc gtgcgcgcgg ctcttgccga
     2101 ggacgtgggc accggggaca tcaccgcgga gctgatcgcg cctgacgccc gggcaaccgc
     2161 agaggttatc acccgggacg agggcatttt ctgcggggcg ccctgggtgc tcgaaacctg
     2221 cgcccaggtg gacgccgcca tcgaggtgac ccttcactgc gcggacggcg atcgcgtctc
     2281 gccggggcag acgctctttg ctctgagcgg ccccgcgagg ggtctgctca cggccgaacg
     2341 gaccatgctg aatttcgttc agctgttgtc agggacggcc acggcgacgc ggcgctacgt
     2401 cgatctgatc gcgggcacgg cggctcggct gctcgacacc cgcaagacgg ttcccgggct
     2461 gcgcatggcg cagaagtacg ccgtgcactg cggcggcggg cacaatcacc ggatcgggct
     2521 cttcgacgct tttctgatca aagagaatca tctggctgcc gccggcggta tcgcgcccgc
     2581 cgtggccaga gcccgcgagc tctacccgga ccggcccgtg gaagtggagg tggagtccct
     2641 ggacgagctc gaccaggccc tcgccggcgg ggcggacatc gcgctcatcg acaatttttc
     2701 gctcgaggat acgtctgccg ccgttacgct ggctgcagga cgcatagagc tcgaggcttc
     2761 cggcggtatc gatgaggcca cgatcacgga aatcgcccgc gccggcgtgg actatatctc
     2821 cctcggcgtt atcaccaaga gagtcattcc gctcgacctg tccatgcgct tcacgagctg
     2881 acgatcatga cccgtatcag cgcctttctg atccacctcg gaatcagctt cgcgatcttt
     2941 ttagggcttg cctatctggt cgttttcatc tggtaccccg acttcttctt cagcaccgac
     3001 ggcggctggc agggaattcg catcatcgtc gccgtggatc tggtgctcgg ccccgcgctg
     3061 acgctgatcg tcttcaagcc cggcaagccg ggactgaaat tcgatctgac cctgatcgga
     3121 ctgtttcagg cagcgtgcct gatcgccggt gtgtacgtgg tccactccga gagaccgctg
     3181 gccatcgtct atgtcgacgg gcagttcttc agcatgagcg gcgacgacta ctccggggtc
     3241 ggattgccca tacccgacct gcgggccgtt ccggggaagt cgcccaagtg ggtcacggtg
     3301 gaaattccac acgacccgtg gatctcgcag gaaatacgca tgagcgccat gaggaaggga
     3361 acacctctgc gcacgcttgc cgatcggtac gcaccgtttt ccgtagagga tgtcgacctg
     3421 gagagggaag cgtatcccct gagcgaactc gtcgaccgcg atcagctgac tcagcagatc
     3481 cccctctggc ttgacgagca cggcggcacc ctcgaggact acgcgttcgt tcgattggcg
     3541 acccgatacc ggtacgtttt cctcgggatc gacaggcggg acggggcgat cgccggcgtg
     3601 ttgggaacgc ccgctccgct gtgagccgac tcaaaaaaac cagcgctgtg cgatcacggg
     3661 ctgggtgttt tccacttcct gctgacgcgc ctcgagctcg ctgacaaact cactatcgcc
     3721 ccgtgcccgg gccatgccga gcaacgcctc ggcgtaacga gcggccgccg gcttgttttg
     3781 acgagccagc aaacgaagcc agggatatat gcgcaggcgc agcagggcct cggcttcggc
     3841 atgacgcccc tcatcgccga gcttgtccat gagcgcgtcg atacccggca cgaactgagg
     3901 gtccatgacc agcgtcctca gcagcagctc ctctggcgcc tcgccgtttt caagcagggg
     3961 caataccgac gggttgcgca tgatgaagtc ggccatcaaa agcagcgtca gcgtgttcca
     4021 cgggtcagcc cgccccgccc ggcgaaaagc attcagcgtc tcctcgccca gatacctgga
     4081 ctccgggttc tgtcgcagtt tgttgtccag cagcacggct tccgccagca ggggcacccc
     4141 ccgatctgcg ttgagggacc gggccatacc cgcaaagcga agcatgcgct ctggactctt
     4201 gcgcacctcc tccgtgaagg gcacgtagcg ctggttctgc agtacgccgg ctgtggcggt
     4261 atccagaccg agatagagcc aggccgtcca tccaaacaga accgccaacg taaccccaac
     4321 acgaatcggt cgcgcctgag tcgagtcaac ggacccggat cctttcgaat cacgaaacga
     4381 cgctgccaca gtaagaccca gcacgaagcc gagagcgggt gagtagacga cgaagttcac
     4441 cagggcgtgc gcgagcgcgg caccaacgcc catggtcagc ccgagcctgg cgaaagcctc
     4501 atctccaggc aaggcggcaa ggcggcgaaa aaacacgatg accacgacga ccgcgaaggt
     4561 caccatggcg cagaacagta acgggccgcc ttccagcagc agctgcagat aatcgttgtg
     4621 aacatatagt ccggccgaat cctggtctac gaccgggcgc tggaaccgat agagcagggg
     4681 aaagacgaac aacccgatgc cggtcgcagg atactcggca tacatggcca gcgccgagcc
     4741 aatcaaagcc attcggacct caatatcggc acctgcaaca cggtccgccg cggctgcctg
     4801 ggcagtcagg gccacggact gatccgtcag agaggctccc gccagcagat acgccgcggc
     4861 ggtcaacgcg gccgcagcag ccacaggccg ccatctcaat cgtctggcta tcgcgagccc
     4921 ggcccaaata atgaaggccc ccgcgatgac gagcaggcct gcgcgggatc ccgtggcaaa
     4981 aagcgcgagc gccaggatga agcatgccga cagatccttc ccttctaccc tcaacgacat
     5041 gccttgggac ccggtcctca gcacaaagta acgatgcagc catggcagga gaatcaggca
     5101 caggagggtc gcatagttgt tggggtccgt caggggcaga cgtgcacgca cgcctgcgct
     5161 gaacagcaat acgacagaga tcgccgcaaa ggccagcacc gtcagggata cggccttgtg
     5221 caacgcaaac gcagcctccc ctgcgccccg ggcaaggaga aaggcgagcg gcgtgacggc
     5281 aaccacccac gccggaacga agctggagtc cttggaaagg ctgaactggt agctgacgac
     5341 gatgccgaca atggccaagg ccggcagcgc gaaagtcaca ggcgaggcgt ggacctctgt
     5401 tgcaaggcgc ggcgccacca acgaaagccc gctcatcaga acgaagatcg cgccaaagaa
     5461 gacgacgaca ttgacgtcgc tgccgtaaaa cacgcagagg agaaggaaac aaacccatcc
     5521 ggcggctaca gccgcctggc acgccagatc tcctcgggga gcgctgtccg gtagcgagat
     5581 catagtggtt ccatgagctg tggtgccgga acgcggaatc gaaccgcgga cctactgatt
     5641 acgaatcagt cgctctacca actgagctat tccggcattt gcctggcccc agagggcgcg
     5701 cagaatagcc ggttttcaga cgggacggaa ggtcaactcg gctcggtggg gagctcaggg
     5761 gcagcggact ttttgaggaa agcgcatgca gaccgtcacg ccggtgtcat ccgtcgccgt
     5821 cacgtcgtag ctcccgccgc cgccgtcggc gatggagtag gaaagctcgc cgtcatctct
     5881 cggcgtccag ccgatgccgg cggtgatcgc cgccgtgttc gccgcggtca gcagatagct
     5941 gcccgttcgc agcatgcggt cttcctgaaa gatctcgatg gtcgagatgt tgttgaccag
     6001 aaccccctct cgcgaggttt gtatgtagcc ctggtacaac ggcagcgcta ctgcagcaat
     6061 gatcgcaatg atcgcgacgg cgatcatcag ttcgatcagg gagaatccgt gactcgttcg
     6121 tgctttgatc attgccatgc tcccctgatc gggtttaccg gttccagccg gcaacaagac
     6181 gagggaccc
//
LOCUS       DBNHLM010000094         9103 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1150589, whole genome shotgun sequence.
ACCESSION   DBNHLM010000094 DBNHLM010000000
VERSION     DBNHLM010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9103)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9103)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9103
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1150589"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="AC16QF_04440"
     CDS             <1..237
                     /locus_tag="AC16QF_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014619.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HIR6447832.1"
                     /translation="DVAEAVRQSGPEAEAYLRPGGRADKYQLDLAGLAVRQLGDVGIG
                     CVERSSACTWSDARFYSYRRDGETGRQATLVWIR"
     gene            complement(276..755)
                     /locus_tag="AC16QF_04445"
     CDS             complement(276..755)
                     /locus_tag="AC16QF_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR6447833.1"
                     /translation="MMRKFISLAFAALCIPVLATADVYRWVGPDGVVNYTQMKPEGVQ
                     AERVQAQGGRRSTAAPAPSEPADQSATQSADTSQLSPKQQEMLNDLQSAEAARKQEVA
                     RIRESNCERSRRVLDRLSATARIRVRDDSGEERIMGEDERQNRIAEAQQGVAENCLS"
     gene            1084..2853
                     /locus_tag="AC16QF_04450"
     CDS             1084..2853
                     /locus_tag="AC16QF_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020296510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HIR6447834.1"
                     /translation="MGEELSGGDILIRALQDAGVDYIFGYPGGSALHIYDAIFRQQKV
                     EHILVRHEQAATHMADAYARATGKPGVVLVTSGPGATNAITGIATAHMDSIPMIVISG
                     QVPNPLIGTDAFQETDMVGISRPVVKHSFLVKDCSEIASVVKKAFHIATTGRPGPVVI
                     DVPKDTTDPDITAPYAFPQSVSLRSYSPAVRGDGRQIRKAVDVLLEAKRPIIYSGGGV
                     IQGDGSELLTRIARRLGFPVTNTLMGLGGFPGDDEQFLGMLGMHGTYEANMAMHNADL
                     ILAIGARFDDRVTNNPSKFSPAATVVQVDVDPASISKIIPADIPVVGQVASVLEEMWA
                     ILESELERRPHLPDAEALKAWWGEIDGWRQAHGLDHSLQPEPGKPILPQLVVQTLYRV
                     TQGEAYVTSDVGQHQMFAAQYYKFSQPRRWINSGGLGTMGFGLPAAMGVQFAFPDARV
                     VCVTGEGSFMMNMQELSTCLQYGLPIKIVNLNNQALGMVKQWQDMQYGGRHSQSTYSD
                     SLPDFKTLVEAFGHVGFKVDRAEDLEPVMIEAFSPPLRERLVFVDVWVDPHEHVYPMA
                     IKGGAMCDMYLDKITTSQQGDYI"
     gene            2853..3344
                     /gene="ilvN"
                     /locus_tag="AC16QF_04455"
     CDS             2853..3344
                     /gene="ilvN"
                     /locus_tag="AC16QF_04455"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR6447835.1"
                     /translation="MRRIIAVLLENEAGALSRVVGLFAQRNYNIDSLTVAPTEDPSLS
                     RLTLTTVGDDRKIEQIIKHLYKVIEVIKVVDLSETDHVERELMLVKVRASGADRSEVK
                     RTCDIFRGQIIDVAPDAYTIQLSGPSEKLDAFIRAIDRALVMEVARTGVSGVSRGDQV
                     LSV"
     gene            3428..4447
                     /gene="ilvC"
                     /locus_tag="AC16QF_04460"
     CDS             3428..4447
                     /gene="ilvC"
                     /locus_tag="AC16QF_04460"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR6447836.1"
                     /translation="MQVYYDKDADLSIVQKMNVLVVGYGSQGHAHANNLRDSGVANVT
                     IGLRSDSGSARKATNAGFQVADVADAAKDADLIMILTPDEHQPQIYRDQIAPNVKQGA
                     TLAFAHGFNIHFELIEPRADLDVIMIAPKGPGHTVRSTYVEGGGVPSLIAIFQDASGQ
                     AKNTALSYASANGGGRAGIIETTFQEETETDLFGEQAVLCGGAAALVQAGFETLVEAG
                     YAPEMAYFECLHELKLIVDFFYEGGITNMWYTVSNTAEYGGLTRGPRLVNEETKQEMR
                     RILEEIQTGRFAQEFVAENQAGAPRIKAMRRISQAHEIEQVGERLRAMMPWIQENRLV
                     DKDKN"
     gene            4532..5335
                     /gene="pssA"
                     /locus_tag="AC16QF_04465"
     CDS             4532..5335
                     /gene="pssA"
                     /locus_tag="AC16QF_04465"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HIR6447837.1"
                     /translation="MSDSEGNQERPPLHCVEEEAGSGSGAASANRMRGIYLLPNLITT
                     GALFAGFYAIVAGMNEHLTAAALAIFAAMALDTADGRVARRTRTESQFGAEYDSLSDM
                     VAFGVAPALVAFSWGLGDALGRIGWVVTFVYMACAALRLARFNVKSDVSSFTGLASPS
                     AAAIIACSVWAWSDNIGGDPGRAAAAGMGAITVVLGLLMVSNFTYYSPKALNMKGRVP
                     FVTMVLVALIFAVTLADPPLVLLFLFSLYALSGPGQWAWGRLTRRGSKG"
     gene            5405..6331
                     /gene="msrP"
                     /locus_tag="AC16QF_04470"
     CDS             5405..6331
                     /gene="msrP"
                     /locus_tag="AC16QF_04470"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HIR6447838.1"
                     /translation="MLIRRPDDVRPSEITPEQVYLNRRKFAKALGAGAAMLAAPWSAN
                     AGDSSGNDLEPTPEDIVTSYNNFYEFGTGKEDPKKHAHALTTSPWSVEVSGAVGKPGT
                     YDLDDLLKGLAPEERIYRFRCVEAWSMVVPWLGIPLKSILDRLEPLGSAKYVEFKTLY
                     RPSEMRGQRSPFSSIDWPYVEGLRLDEANHPLTIMAVGLYGKPLPNQNGAPWRLVVPW
                     KYGFKSIKSIVSIRFTEREPENTWKLLQPQEYGFYANVNPAVDHPRWSQASERRLPSS
                     LFSQNRMPTLPFNGYADEVAGLYAGMDLTRYY"
     gene            6336..6935
                     /locus_tag="AC16QF_04475"
     CDS             6336..6935
                     /locus_tag="AC16QF_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013918.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016679 - oxidoreductase activity, acting
                     on diphenols and related substances as donors [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HIR6447839.1"
                     /translation="MRRRLLFTALCLPLAWLGYAIAGELNQPGSVLGADPGEAVVLFL
                     GEWGLRLLLLTLAVTPLRRLTGWAELLGYRRMIGLFSFTYLALHLLAYVALLAEFDWR
                     LVLEDFSDRAYITAGLIGFGLLLPLAVTSTRGWQRRLGRRWRSLHRLVYPAVFLGLLH
                     LVWLTKDGYGEVAAYGVLLSLLMVERIRHGAWWGRKAAG"
     gene            6937..7272
                     /locus_tag="AC16QF_04480"
     CDS             6937..7272
                     /locus_tag="AC16QF_04480"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR6447840.1"
                     /translation="MRCEPCRGDSPAVAPDAREAMLAELTGWRVDVVDEVPRLSRTYQ
                     TADFAAALGLANTIGEMADSEDHHPQLVVEWGRLQVSWWTHAIGGLHMNDFVLAARCD
                     ELAAKAASD"
     gene            complement(7269..8354)
                     /locus_tag="AC16QF_04485"
     CDS             complement(7269..8354)
                     /locus_tag="AC16QF_04485"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR6447841.1"
                     /translation="MKYLTDDVRIIGMEEVTAPETLLSELPITDSVSRLVFSNRKQIS
                     EILHGSDPRLLVVVGPCSIHDPEAALDYAKRLAPVAKDLSDRLHVIMRVYFEKPRTTV
                     GWKGLINDPHLDNSFDINNGLRIARGVLRDIAECGLGAGTEYLDPITPQYVGDLVAWG
                     AIGARTTESQIHRQLASGLSCPVGFKNSTDGSIQVAVDAIKSAANSHIFLSVTKSGHS
                     AIFSTAGNDDCHLILRGGGGKPNYDNPSVHYASRLLEQAGLTSRLMIDMSHANSGKDH
                     NQQIRVCQDICKQVREGESRIFGVMIESNLVPGNQKIGPRESMTYGQSVTDACIGWED
                     TVTCLTALADAGGARMEGSAAAAAGSG"
     gene            8464..>9103
                     /locus_tag="AC16QF_04490"
     CDS             8464..>9103
                     /locus_tag="AC16QF_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447842.1"
                     /translation="MTEAVRLDARRRGMLADMGIDVWVIRGNAAGVVEVPQSQTIVVP
                     EPVPPAETKAPEKGPDVARLNDSAPSAAQQAKMPVPRRRPLEHPEADVATEIAVTCLT
                     RPGALLLAYGREWTQAGRLGRDLLAAATGSWGSDPKRISFGWREPGDPSDGWRAFKAF
                     ADKQLADAEASVIFVNEALVDHLPERASSGTLVVLPNLAEFDTDAKRGLWTRI"
BASE COUNT         1615 a         2773 c         3029 g         1686 t
ORIGIN      
        1 gacgtggccg aggcggtgcg gcaatcgggc cccgaggcgg aagcctacct gagaccgggc
       61 ggacgggcgg acaaatatca gcttgacctg gccgggctgg ccgttcggca gcttggggac
      121 gtggggatcg gatgtgtcga gcgatcctcg gcatgcacct ggtccgacgc caggttttat
      181 tcgtatcggc gcgatggcga gaccggtcgc caggcaacgc tggtctggat acggtgagct
      241 ggcagtgggc ccgcacggct gcgggccccg ggtcctcagg acaagcagtt ctctgcaacg
      301 ccctgctggg cttcagcgat gcgattctgg cgctcatcct cgcccatgat tctttcctcg
      361 ccggaatcgt cacgaacgcg tatgcgggcg gtggcggaca ggcggtcgag cacccgtcgt
      421 gaccgctcgc agttggattc ccggatccgg gcgacttcct gcttgcgggc ggcctcagcc
      481 gactgcagat cgttcagcat ttcctgctgc ttaggggaga gctgtgacgt gtccgcgctc
      541 tgggtggcgg actggtcggc gggctcgctt ggggctgggg cagcagtcga acgtcttccg
      601 ccctgggcct ggacgcgctc ggcctggaca ccctcaggtt tcatctgagt gtagttgacg
      661 accccgtcgg gaccgaccca gcggtagacg tcggcggtcg ccagtaccgg tatgcagaga
      721 gcagcgaagg cgagactaat gaacttgcgc atcatccaga tactcctgat atatgccacg
      781 gccgccggcg gtttttttcg tgaccggcgc ccgggcgtag caatccaatc cgggatttta
      841 gagcgaccgg ggcgtccgtc ccttgcggca gttaccccgg atatgtgaag cgattcgcaa
      901 tttggcgcat tggaccgttc gttgacatgc cgtgggcgaa ccccaagaat acgcggctcc
      961 ggcggtccct cctttcaaac cccgtgaaaa atcaaacgtt tatgggtggc gggacatcag
     1021 ccggtgaggt atctggttga ctaacgggca cgaatcacag tgggcacaga caagagtcag
     1081 agcattggcg aagagctctc cggcggcgac atcctgatac gggcgctgca ggacgccggc
     1141 gtggattaca tcttcgggta cccgggcggc tccgccctgc acatctatga cgccatattc
     1201 cgccagcaga aggtggagca catcctggtg cgtcacgagc aggccgcgac gcacatggcg
     1261 gatgcctacg cgcgcgccac ggggaagcct ggcgtcgtgc tggtgacctc gggcccgggc
     1321 gctaccaacg ccatcaccgg aatcgccacg gcccacatgg attccattcc catgatcgtg
     1381 atctcgggac aggtccccaa cccgctgatc ggaacggatg cgtttcagga aacggacatg
     1441 gtcggtatct cgcggccggt ggtgaagcat tcgttcctgg tcaaggattg cagcgagatc
     1501 gcttcggtgg tcaagaaggc gttccacatc gccaccaccg ggcgtcccgg tccggtggtc
     1561 atcgacgttc caaaggacac cacggacccc gacatcaccg cgccttacgc cttcccgcag
     1621 tcggtgagct tgcgttccta cagtccggcg gtcaggggtg acgggcggca gatccgcaag
     1681 gccgtggacg tgctgctgga agccaagcga ccgatcatct actcgggtgg aggcgtgata
     1741 cagggcgacg ggtcggaact gctcacgcgc attgcccggc gcctgggctt cccggtgacc
     1801 aatacgctga tgggtctggg cggatttccc ggcgacgacg agcagttcct cggcatgctc
     1861 ggcatgcacg ggacctacga agcaaacatg gccatgcaca acgcggatct gatcctggcc
     1921 atcggcgcgc gctttgacga ccgtgtcacc aacaacccga gcaagttctc gcccgcagcc
     1981 accgtggtgc aagtcgatgt ggatccggct tccatctcca agatcatccc ggcggacata
     2041 cccgtcgtcg ggcaggtggc gagcgtgctc gaagagatgt gggcaatcct ggaaagcgag
     2101 ctcgagcggc gtccgcatct gccggacgcc gaagcgctca aggcatggtg gggcgagatc
     2161 gacggatggc ggcaagcgca cgggctcgat cactctcttc agccggagcc gggcaagccg
     2221 atcctcccgc agctggtggt gcagactctc tatcgcgtca cccagggcga agcctacgtg
     2281 accagcgatg tgggccagca ccagatgttt gcggcgcagt attacaagtt ctctcagccg
     2341 cggcgctgga tcaactccgg cggcctcggc accatgggct tcgggctgcc cgctgccatg
     2401 ggcgtgcagt ttgcctttcc ggatgcgcgg gtggtctgcg tcaccggcga gggaagcttc
     2461 atgatgaaca tgcaggagct gtccacctgc cttcagtacg gtctgccgat caagatcgtc
     2521 aacctcaaca accaggccct gggaatggtc aagcagtggc aggacatgca gtacggcgga
     2581 cgtcactctc agagcaccta cagtgactcg ctgccggact tcaagaccct ggtggaagcg
     2641 ttcggccatg tgggcttcaa ggtggaccgg gccgaggatc tggagccggt aatgatcgaa
     2701 gctttctcgc cgccgctgcg tgagcgcctc gtgttcgtgg atgtctgggt cgatccccac
     2761 gagcacgtct acccgatggc catcaagggt ggcgccatgt gcgacatgta tttggacaag
     2821 atcaccacca gccagcaggg agactacatc tgatgcggcg catcatcgcg gtgctgctcg
     2881 aaaacgaggc gggagcgctg tcccgggtgg tggggctctt cgcccagcgc aattacaaca
     2941 ttgacagcct caccgttgcg cccacggaag atccctccct gtcgcggctg acgctcacca
     3001 ccgtgggcga cgaccgcaag atcgagcaga tcatcaagca cctgtacaag gtgatcgagg
     3061 tcatcaaggt ggtcgatctt tccgagactg accacgtgga gcgcgagctc atgctcgtaa
     3121 aggtgcgcgc atctggtgcc gaccgctcgg aggtgaagcg tacgtgcgac atattccgcg
     3181 ggcagatcat cgacgtcgcg cccgatgcgt acacgattca gctctcgggg ccgagcgaaa
     3241 agctcgatgc cttcattcgg gccatcgacc gcgcgctcgt catggaggtg gcccgcacag
     3301 gcgtctccgg cgtcagccgt ggcgaccagg tgctgagcgt ctagggcggg ccatgaacgt
     3361 ctgcggatcg ctgatgttcg tggcggacaa gaatttgcaa tccggatctg gaaaaggggg
     3421 aagcgacatg caggtttact acgacaagga cgcggatctg tcgatcgtcc agaagatgaa
     3481 cgtcctggtg gtgggctacg gctcccaggg ccacgctcat gccaacaatc tgcgcgactc
     3541 gggggttgcc aacgtgacca tcggcctgcg cagcgattcg gggtctgcgc gcaaggcgac
     3601 caacgcggga tttcaggtgg cggacgtggc cgacgccgcc aaggacgccg acctgatcat
     3661 gatactcacg cccgacgagc accagccgca gatctaccgg gaccagatcg cgcccaacgt
     3721 caagcaaggc gccactctgg ctttcgccca cggcttcaac attcacttcg agctcatcga
     3781 gccgcgcgcc gatctcgacg tcatcatgat cgcgccgaaa ggacccggcc acacggtgcg
     3841 ctccacttac gtcgagggcg gcggggtgcc gagcctcatc gcgattttcc aagacgcgag
     3901 cggccaggcc aagaacacgg cgttgtccta cgcgtcagcc aacggcggcg ggcgtgctgg
     3961 aatcatcgag accacgtttc aggaagaaac ggagaccgac ctctttggcg agcaggccgt
     4021 gctctgcgga ggcgctgcgg ccctggtcca ggccggtttc gaaaccctgg tggaggccgg
     4081 ctacgcaccc gagatggcct atttcgaatg cctccacgag ctgaagctca tcgtcgattt
     4141 cttctacgaa ggcggtatca ccaacatgtg gtacaccgtg tccaacacgg ccgagtacgg
     4201 cggcctgacc cgtggtccgc ggttggtcaa cgaggaaacg aagcaggaga tgcgcaggat
     4261 tctcgaggag atccagacgg gcagattcgc tcaggagttc gtggcggaaa accaggcggg
     4321 agcgccccgc atcaaggcca tgcggcgtat cagccaggcc cacgagatcg agcaggtggg
     4381 tgagcggctg cgtgcaatga tgccctggat ccaggagaat cgtctggtgg acaaggataa
     4441 aaactgacct gcgctgcaac gacgcggtgc tccaagcgca gcgctcgcca cgggattgtc
     4501 ggcaaacgct gcccgcccgg aagggggtaa tttgagcgat agcgaaggta atcaggagcg
     4561 cccgccgctt cactgcgtgg aggaggaagc agggtcgggc tccggggccg cctctgccaa
     4621 caggatgcgc ggtatctacc tgctgccgaa tctaatcacc accggcgctc tctttgccgg
     4681 gttctacgcc atcgtggcgg ggatgaatga acatctcact gcagccgccc tcgccatatt
     4741 tgccgccatg gcgctggata cagccgacgg ccgggtagcc cgacgtaccc gtacggagag
     4801 tcagttcggc gccgagtacg acagcctttc cgacatggtg gctttcggcg tggcgccggc
     4861 gctggtggcg ttctcatggg ggctcggaga cgcgctgggc cgcatcggct gggtggtgac
     4921 gttcgtgtac atggcgtgtg ccgcgctgcg cctggcgcgt ttcaacgtca agagcgacgt
     4981 cagttccttt accggcctgg ccagcccatc tgctgcggcc atcatcgcct gctcggtatg
     5041 ggcctggagc gacaacatcg gaggtgatcc gggccgcgcg gcggcagccg gcatgggagc
     5101 catcacggtg gttttggggc tgctgatggt ttcgaatttc acctactact cgccgaaggc
     5161 gttgaacatg aaaggccggg tgccgtttgt caccatggtg ctggttgcgc tgatattcgc
     5221 cgtcaccctc gcggacccgc cgctggtctt gctgttcctc ttctcgctct atgccctttc
     5281 gggtcctggg cagtgggcct ggggtcggct gacccgccgc gggtcgaaag gctgagaacg
     5341 gttgccggga accgggacgt ccggtggggt ctaacgttgg gaatctggct gttctggaaa
     5401 tgacgtgctg atccgcagac ccgacgacgt ccgaccctcg gagatcacac ctgagcaggt
     5461 ctatctgaac cggcgcaagt ttgccaaagc gctcggtgcg ggggcggcca tgctggcggc
     5521 gccctggagt gcgaacgctg gagactcgag cgggaacgac ctcgagccga cgcccgaaga
     5581 tatcgttacc agctacaaca acttctatga gtttggcacc ggaaaggaag accccaagaa
     5641 gcacgcccac gcgctgacca catcgccctg gtcagtggaa gtttcggggg cggtgggcaa
     5701 accgggcacc tacgatctgg atgatcttct caaagggctg gcgccggagg aacgcatcta
     5761 tcggtttcgc tgcgttgaag cctggtcgat ggtcgttcca tggcttggca ttcctctcaa
     5821 gtccattctc gaccgccttg agcccctcgg cagcgcgaag tacgtcgagt tcaagacgct
     5881 gtaccgcccc agcgagatgc gtggccagcg ctcgccgttc agcagcatcg actggccgta
     5941 cgtggagggc ttgcggctgg acgaggccaa tcaccccctc acgatcatgg ccgttggcct
     6001 ctacggcaag ccgctgccga accagaacgg ggcgccgtgg cgcctcgtcg tgccgtggaa
     6061 gtacggcttc aagagcataa agtccatcgt ctccattcgc ttcaccgagc gtgagcctga
     6121 gaacacctgg aaattgcttc agccgcagga atacggcttc tacgccaacg tcaatccggc
     6181 ggttgaccac ccgcgctgga gccaggcatc ggaacgacgg ctgccgagct ccctgttctc
     6241 gcagaaccgg atgccgacgt tgcccttcaa cggctatgcc gatgaggtgg cgggtctgta
     6301 tgcggggatg gatctcaccc gctattactg agcccgtgcg cagacgcctg ctgtttacgg
     6361 ccctgtgcct gccgctcgcg tggctgggtt acgcgatcgc cggcgagctg aatcagcccg
     6421 ggagcgtgct tggggcggat ccgggagagg ctgtggtgct ctttctcggc gagtggggct
     6481 tgcgcctgct tcttctcaca ctggcggtca cgcccttacg gcgcctgacc gggtgggcgg
     6541 agctgctcgg ttatcgccgg atgatcggtc tgttcagctt cacctacctg gctttgcacc
     6601 tgcttgccta cgtcgcgctg cttgccgagt tcgactggcg tctggtgctc gaggacttct
     6661 ctgatcgagc ctacatcacg gccgggctca tcgggttcgg gctgctgttg ccactggctg
     6721 tcacgtcgac ccgcggctgg cagcgccgcc tgggtcgccg ctggcgcagc ctgcaccggc
     6781 tggtctaccc ggccgtgttc ctcgggctgc tccatctggt ctggctcacc aaggacgggt
     6841 acggcgaagt tgcggcatac ggcgtactat tgagtctatt gatggtagag aggatcagac
     6901 atggcgcatg gtggggacgc aaagcggctg gctgagatgc ggtgcgagcc ttgtcggggc
     6961 gattcgccgg cagtggcgcc ggatgcgcgg gaggctatgc tggccgagct caccggctgg
     7021 cgggtcgacg tggtggacga ggtacccagg ctcagccgga cctatcaaac cgccgacttc
     7081 gccgcggcgc tggggcttgc gaacaccatc ggcgagatgg ccgacagtga ggatcaccat
     7141 ccgcagctgg tggtggagtg gggccggctg caggtcagct ggtggacgca cgccatcggc
     7201 ggcctgcaca tgaacgactt cgttctcgcg gcccgatgcg acgagctggc tgccaaggcg
     7261 gcttcggact agcccgatcc tgccgctgca gcggccgagc cttccatccg cgccccgccc
     7321 gcgtcggcca gcgcggtcag gcaggtgact gtgtcctccc acccgataca cgcatcagtg
     7381 acgctctggc cgtacgtcat ggattcacgt gggccgatct tctggttgcc cgggaccagg
     7441 ttgctctcga tcatcacgcc gaaaatccgt gactcgcctt cgcggacctg cttgcagata
     7501 tcttggcaga cccggatctg ctggttgtga tccttgccgc tgttggcgtg gctcatgtcg
     7561 atcatcaggc ggctcgttag ccccgcctgc tcgagcagac ggctggcata gtggaccgaa
     7621 gggttgtcgt agttcggctt gccgcccccg cctcgaagaa tgagatggca atcgtcgttg
     7681 ccggccgtgg aaaagatggc cgagtgcccg gacttggtga cggagagaaa tatgtgggaa
     7741 ttggcggccg acttgatggc gtccacggcg acctggatgg agccgtcggt gctgttcttg
     7801 aaacccacgg ggcaggacag cccggaagcg agctggcggt gaatctggct ctcggtggtg
     7861 cgggctccga tagcgcccca ggctaccagg tcgccgacat actgcggcgt gatcggatcc
     7921 aggtactccg tgcccgcgcc cagtccgcat tcggctatgt ctctgagcac gcccctggcg
     7981 atcctcagac cattgttgat gtcgaaggag ttgtcgagat gggggtcgtt gatcaggccc
     8041 ttccacccca cggtcgtgcg cggtttttcg aagtagaccc gcatgatgac gtgaagacgg
     8101 tcggagaggt ctttcgccac cggggccagc cgctttgcat agtccagggc agcttccggg
     8161 tcatgaatcg agcagggccc gacgaccacc agcaatcggg ggtcgctgcc gtggagaatc
     8221 tcgctgatct gcttgcggtt ggagaagacc agtcgcgaaa cggaatcggt gatcggcagc
     8281 tcggaaagga gggtttccgg cgcggtgacc tcttccatgc cgatgatgcg tacgtcgtct
     8341 gtcagatact tcattgggaa ccatcacggt gcgtgagctc ggaagctaga gtgtacaatg
     8401 accgcagagg cagggttacc ggcaatcgcg gcatgtatac caaagttctg aacgggtgcg
     8461 ggcatgactg aagccgttcg tctggatgcg cggcgtcggg ggatgctcgc ggacatgggc
     8521 atagacgtct gggttatcag gggtaacgcg gccggggtcg tcgaggtccc gcagtcgcag
     8581 accatcgtcg tgcccgagcc cgtgccgccg gccgagacga aggcgccgga gaagggcccc
     8641 gacgttgcca ggctgaacga ctcggcgcct tcggcggccc agcaggcgaa gatgccggtc
     8701 ccgcgccgga gacccttgga acatccggaa gcggatgtcg cgacggaaat cgcggtcacc
     8761 tgtctgacgc gccccggcgc tttgctgctg gcttacggcc gggaatggac ccaagccggc
     8821 aggctcggcc gcgacctcct cgctgccgcg accggcagct ggggctcgga tccaaagcga
     8881 atctcattcg gctggcggga gccgggcgat ccttcggacg gctggcgggc cttcaaggca
     8941 tttgcggaca agcagctggc ggacgccgag gcttcggtga ttttcgtaaa cgaagcgctg
     9001 gtcgatcacc ttccggagcg ggcgagctcc gggacgctgg tcgtgctgcc gaacttagcc
     9061 gagttcgaca ctgacgccaa acgaggccta tggaccagga tcc
//
LOCUS       DBNHLM010000095         7160 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1269958, whole genome shotgun sequence.
ACCESSION   DBNHLM010000095 DBNHLM010000000
VERSION     DBNHLM010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7160)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7160)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7160
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1269958"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..712)
                     /locus_tag="AC16QF_04495"
     CDS             complement(224..712)
                     /locus_tag="AC16QF_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HIR6447843.1"
                     /translation="MFYDGRFAAVATTMQIWVDADACPGPIKEILFRAADKRGVQLTL
                     VANHPMRVQKSAFIRLLQVEQGFDKADAFIADRVEAGDLVITADIPLAADVVEGGGAA
                     LNPRGTLYTEENIREHLARRNFMEELRSTGQISGGPAALGKPDVQKFANSLDRYLTRA
                     VG"
     gene            complement(745..1425)
                     /locus_tag="AC16QF_04500"
     CDS             complement(745..1425)
                     /locus_tag="AC16QF_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447844.1"
                     /translation="MRPWRGTCGWCIGLVGVIAAAAFPAGAVQAAAISIAEARAAEPG
                     TLVTVEGSVTVPSGRFSASTFDQGFAIQDGSAGIYVSVADDPKLNNHRRVRVTGVVGD
                     DGYGQLVLRPGVDADVEILQGAARVAARQVATGDVGEATEGLLVEVTGSVVRGPTDDL
                     PYGYSVYIDDGSGETQIFIPASTGINPFRIGYLDEGVAIRVTGLSSQFQNNLEVLPRT
                     GGDIQRGR"
     gene            complement(1422..2480)
                     /locus_tag="AC16QF_04505"
     CDS             complement(1422..2480)
                     /locus_tag="AC16QF_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6447845.1"
                     /translation="MDYGIALATTMDSWRAARRAEELGFSHAWFYDTQLLNPDVFMGM
                     TLAAVNTSRIRLGTGVLIPSNRIEPVTANALVSLNKLAPGRIDFGVGTGFTGRRTMGL
                     KAIPLERMKTYIERVQALVKGETVTWDFEGETHDIAFLNPELGLINVDDPIALHVSAF
                     GPRSRDLTAELGAGWLNFGVSEPVAFGSLAKMRESWARAGRDAGDLYSTLFSLGCVLK
                     PGEAFDSPRAKAQAGTAVAVFLHDMAETSEPGSLEPVMGKAVSHVVEAYREIYLGYPE
                     GQRHLLNHRGHLMFLKPEEQDLITAELIQAITFTGERAALRERLDAFEAAGYGQFTVQ
                     LIEGHEDAIEDWAEVFFG"
     gene            complement(2494..3699)
                     /locus_tag="AC16QF_04510"
     CDS             complement(2494..3699)
                     /locus_tag="AC16QF_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR6447846.1"
                     /translation="MNTLLIVGAVFLIGLAADVAGRLTPLPRVTLLLLAGVLVGPSVF
                     ALIPSGFIDQWFPTLTHMALAMVGFLMGHQLSVAELRKRGKVVSLLALGKVVGAALVV
                     FVAVWALTGDLALALVLGGVATATAPAATFDVVHEAHARGAFADNLVSIVAIDDAWGL
                     VLFSVLLALAASMANGDALDASIVGAGVMEVLGSLVLGVVIGVPMAYLTGRLTFGERQ
                     GEPIQAESFGFVLACAGGAVLLDLSPILASMAMGSVVASLARHHARPFHAIEGVEWPF
                     MILFFVLAGASLEVEGVSLVGWLALAYLVARALGTHLGIYLTAGIIGTDGPTRRWMGL
                     ALMPQAGVALGMALIASQRLPGHAETILTLVLSTTILLELVSPIITRRILERVGAGGE
                     LPDATDTTN"
     gene            complement(3696..4976)
                     /locus_tag="AC16QF_04515"
     CDS             complement(3696..4976)
                     /locus_tag="AC16QF_04515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6447847.1"
                     /translation="MQTALQLSDSAGVRYATGMAMYFAQGIPKGLLHIAMPAWLASRG
                     VAPGEIATYLAVIVLPWAFKLVTGPLMDRYEFLPMGRRRPWVMFAQLGMVLSLLGLMI
                     VEDPVEQMGTLILLGLVVNSFTATQDVAVDGMSIDFVPAREQGRLNAFMSFGKAIGWA
                     LTSAVSGVMLVTWGLGVTAVAAAAGASVFFVALMFVREQPGERLLPWTAGQAASVHVE
                     GKTFAAVFAGVNRVLWQRGSLIVLAIMFFHGLWNGYGDALMPIAAINLFGYTTPQWSQ
                     LVAVMGLVGAGLALAAGPLIDRFGAKRMLVLTISLVAVHAFLVAQTQHLWQDTTYVRV
                     MLSIWVTMDPVIMVCVIALAMAICSRENSATQFAIYMSAANLGASLGAKAYGLVAEQS
                     TYVENYTLLGVFILVMLFTVVFHRHPLNRQSEPA"
     gene            complement(5066..>7160)
                     /locus_tag="AC16QF_04520"
     CDS             complement(5066..>7160)
                     /locus_tag="AC16QF_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HIR6447848.1"
                     /translation="DQLTVINYAFVVPAPNAAGDVVCQMDDPVAAYQQLYTAAMSVDG
                     IPDVDDAGQPLRGHFNQFKKLKAEHPHLKVLIAIGGWLGSVWWSDAAATPKSRETFVA
                     SCIDLFIRGNLPEAGNAGGVGVAAGVFDGFDIDWEYPITGGAGGTHHSADDDVNLTAT
                     LAEFRRQLDALPRAAGTDPYLLTMATPGSAFRGDNYQINADQAHVDWFNLMTYDFHGT
                     WENKTGHQTNILTSVNDPSSDAFKLSIDNAVRLYRDTYGVSVEKLVIGAAFYGRGWSG
                     VSTTDDGLYQSGRTAPGIYEDGYNYYRDLAPLMLGDYTLHWDPLALASWLYSPVERTF
                     WTLDEPQSIALKRRYADAFGLGGVMAWEVSGDDEDGTLVSAMRTGDPGAPVQSSSSAL
                     DGAVEVTQPASCAISVEGFNQVLGAETTVSAPAKVDQVEFFGNGVSLGFDNFPPWSWA
                     WFNLPAGVHEITAVASDWAGSSTSSTSVLLNVYSEADMALWQVGASYQPGDEVFYDGC
                     IYQAKRNHVGSRVRLPTGGRYWELVTCSDCGGGGGGGGEGGNQPPVIESITASPSTSI
                     RANENVTLTVVAGDPEGWIASVVFAVGAGVESGVYSEFDTWTWTWSNVPAGTHEVTVT
                     AHDNSGATASDRITIVAASGSGCTAPAWDATLTYDKGDRVQHNDILWQAKRTVAEVEP
                     GTSPSKWTNLGPCTS"
BASE COUNT         1287 a         2409 c         2346 g         1118 t
ORIGIN      
        1 gctctgttgg ccccgagact gttactgtcg ataatgtcac ggtttcgaac accgtctcaa
       61 acgggttcgc cgttgcgatt gccgccggcg cctttgcgga cctctgcctc taaccacctc
      121 ttcggcatgg tctccggcag catccgctgt cggggaccaa gttttccgtc cgctacacac
      181 gtctgaacct caacccgtca accatttggt ggcttacgcc gacctaaccc accgccctgg
      241 tcagataccg atccagcgag ttcgcgaact tctgcacgtc gggcttgccc agcgcagccg
      301 ggccgcctga gatctgcccg gtggagcgca gctcctccat gaagttgcgc cgggcgaggt
      361 gctcgcggat gttttcctcg gtgtacaggg tgccgcgggg gttcagcgcc gcgccgccgc
      421 cttcaaccac atcggccgcc agggggatgt ccgcggtgat cacgaggtcg cccgcttcga
      481 cccggtcggc gatgaaggcg tccgccttgt cgaacccctg ctccacctgc agcagccgga
      541 tgaaggccga cttctgcacc cgcatggggt ggttggccac cagggtgagc tgcaccccgc
      601 gcttgtccgc agcgcggaag aggatctcct tgatgggccc ggggcaggcg tcggcatcca
      661 cccagatctg catggtcgtg gcaacggcgg caaagcggcc atcatagaac atccatagaa
      721 catcgaggac cggtggcgtt ggccttatct gccccgttga atatctccac cggtgcgcgg
      781 cagcacttcc agattgttct gaaactggct gctcagcccc gtgacgcgaa ttgccacgcc
      841 ctcatccagg tagccgatcc tgaacgggtt tatccccgtg ctggcgggta tgaagatctg
      901 cgtctccccg gagccatcgt cgatgtaaac gctgtagccg tagggcagat cgtccgtcgg
      961 cccccgcacg acggatcccg tcacctccac cagcagaccc tccgtggcct cgcccacgtc
     1021 ccccgttgcc acctgccggg cggccacgcg ggcagcgccc tggaggatct ccacatccgc
     1081 atcgacaccc ggccgcagca ccagctgccc gtagccgtcg tccccgacga cgccggtgac
     1141 ccgcacgcgg cgatgattgt tcagcttggg gtcatcggca acggagacgt agatccccgc
     1201 cgagccgtcc tggatggcga aaccctgatc gaacgtagac gcggaaaacc ggccggacgg
     1261 gaccgtcacc gagccctcca cggtcaccag ggtgcccggc tccgcggccc gcgcctcggc
     1321 gatggagatc gcggcggcct gaacggcgcc tgcggggaaa gcggcagccg caatgacgcc
     1381 gacaagcccg atgcaccagc cgcaggtgcc ccgccacggc ctcatccgaa gaacacctcc
     1441 gcccagtcct cgatggcgtc ctcgtggcct tcgatgagct ggacggtgaa ctggccgtag
     1501 ccggcggcct caaacgcgtc gagccgctcg cgcagggccg cccgctcgcc ggtgaaggtg
     1561 atggcctgga tgagctcggc ggtgatgagg tcctgctcct cgggcttcaa gaacatcagg
     1621 tgacccctgt ggttcagaag atgacgctgg ccctccgggt agccgagata gatctcccga
     1681 taagcctcca ccacgtggct cacggccttg cccatgacgg gctctaaaga acccggctcg
     1741 gaggtctccg ccatgtcgtg gaggaacacg gccaccgcgg tgcccgcctg ggccttggcc
     1801 cttggcgagt cgaacgcctc gccgggcttc agcacgcagc ccagcgaaaa cagcgtgctg
     1861 tagagatcgc cggcatcccg ccccgcgcgg gcccaggatt cgcgcatctt cgccagcgac
     1921 ccgaacgcca ccggctcgct cacgccgaag ttcagccacc cggcgccaag ctctgccgtc
     1981 aggtcacggg agcgcgggcc gaaggctgag acgtgcagcg cgatgggatc gtccacgttg
     2041 atgaggccga gctcgggatt gagaaaggcg atgtcgtggg tttcgccctc gaagtcccag
     2101 gtcaccgtct cgcctttgac aagcgcctga acgcgctcga tgtacgtctt catccgctcg
     2161 agggggatcg ccttcaggcc catggtgcgc cggccggtga agccggtgcc aacgccaaag
     2221 tcgatgcgcc cgggggcaag cttgttgagg ctcacgagcg cgttggcggt gaccggctcg
     2281 atgcggttgc tgggtatcag caccccggtg ccgaggcgga tgcgcgaggt gttcaccgcc
     2341 gccagcgtca tgcccatgaa gacgtccggg ttcaagagct gggtgtcgta gaaccaggcg
     2401 tggctgaagc cgagctcctc cgcgcgccgg gccgcccgcc aggagtccat ggtggtcgct
     2461 agagcaattc cgtagtccat gtttgcgtcc gtttcagttt gtcgtatcgg ttgcgtccgg
     2521 cagctcgcca cccgcaccga cgcgttccag gatccggcgc gtgatgatcg gcgagaccag
     2581 ctccagcagg atggtggtgc tcagcaccag ggtgagaatg gtctcggcat ggccgggaag
     2641 gcgctgagag gcgatgagcg ccatgccgag cgccaccccg gcctggggca tgagggccag
     2701 acccatccag cggcgggtcg ggccgtccgt gccgatgatg cctgcggtaa ggtagatacc
     2761 gaggtgcgtg cccaacgcgc gggccaccag gtacgcgagg gcgagccagc caaccaggct
     2821 gacgccttcc acctccagcg aggcgccggc cagcacgaaa aaaaggatca tgaaaggcca
     2881 ttccaccccc tcgatggcgt gaaaagggcg cgcgtggtgc ctggcgaggc tcgccaccac
     2941 gctgcccatg gccatggagg caaggatggg cgaaaggtcg agcagcaccg cgccccccgc
     3001 gcaggccagc acgaacccga aagattccgc ctgaatgggc tcgccctgcc gctcgccgaa
     3061 agtcagccgc ccggtgaggt aggccatggg cacgccgatg acgacgccga ggaccaggct
     3121 ccccagcacc tccatcaccc cggcgccaac gatggaggca tccagcgcgt cgccattggc
     3181 catgctggcc gccagcgcca gcagcacgga gaacaggacg aggccccagg catcgtcgat
     3241 ggcgacgatg ctcacgaggt tgtcggcaaa cgctcctctg gcatgagcct cgtgcaccac
     3301 gtcgaaggtg gcggcaggcg cggtggccgt cgccaccccg ccgagcacca gggctagggc
     3361 aaggtctccg gtcagggccc acacggcgac gaacaccacc agcgcggccc ccaccacctt
     3421 gcccagcgcc agcagcgaga ccaccttgcc gcgcttgcgc agctctgcga cggatagctg
     3481 atggcccatg agaaagccca ccatggccag ggccatgtgg gtgagcgtgg gaaaccactg
     3541 atctatgaag cccgatggga tcagagcaaa tacgctgggt ccgacgagaa cgcccgccag
     3601 cagcagcagc gtcacccgcg gcagcggcgt caggcgcccg gccacgtcgg ccgccagccc
     3661 gatcaggaac acggcgccga cgatgagcag ggtgttcacg cgggctcgct ctgccggttc
     3721 agcgggtgcc gatggaacac cacggtgaag agcatgacca ggatgaacac gccaaggagc
     3781 gtgtagttct ccacgtaggt ggactgctcc gccaccaggc cgtaggcctt ggcacccagc
     3841 gaggcgccga ggttggccgc ggacatgtag atggcgaact gggtggccga gttctcccgg
     3901 gagcagatgg ccatggcgag cgcgatcacg cagaccatga tgaccgggtc catggtcacc
     3961 cagatggaga gcatcacgcg cacgtaggtg gtgtcctgcc acaggtgctg ggtctgcgcc
     4021 acgaggaagg cgtgaacggc caccagcgag atggtcagca ccagcatgcg cttggcgccg
     4081 aagcggtcga tgaggggccc ggccgccagc gccaaacccg cacccaccag gcccatgacg
     4141 gccacgagct gggaccactg gggcgtggtg tagccaaaga ggttgatggc cgcgatgggc
     4201 atgagcgcat cgccgtagcc gttccagagc ccgtgaaaga acatgatggc cagaacgatg
     4261 aggctccccc gctgccatag cacccggttc acaccagcaa agacggcagc gaacgtcttg
     4321 ccctccacgt gcacggaggc cgcctgaccg gccgtccagg gaagcagacg ctcgccgggc
     4381 tgctcccgga cgaacatcag ggcgacgaag aagaccgacg cgccggccgc ggccgcgacc
     4441 gcggtcaccc cgaggcccca ggtcaccagc attaccccgg aaacggccga ggtcagcgcc
     4501 cagccgatgg ctttgccgaa gctcatgaac gcgttcagtc gcccctgttc ccgggcggga
     4561 acgaagtcga tggacatgcc gtccacggcc acgtcctggg tcgccgtgaa cgagttgacg
     4621 accaggccga gcaggatgag cgtgcccatc tgctcaacgg gatcctcgac gatcatgagc
     4681 ccgagcaggc tcagcaccat gccgagctgc gcaaacatca cccagggccg gcggcggccc
     4741 atgggcagaa actcgtagcg atccatgaga gggccggtga ccagcttgaa cgcccagggc
     4801 agcacgatca cggcaaggta ggtggcgatc tcgccggggg cgacgccacg gctggcgagc
     4861 caggccggca tggcgatgtg cagcagcccc ttgggaatgc cctgggcaaa atacatggcc
     4921 atgccggtgg cgtaacgcac accggcgctg tcagacagct gcagagcagt ttgcacgatg
     4981 cttttgagcc ctcaccccga agacgcgatg ataccccgtc cgccgctatt ttagagcgct
     5041 ttgagcctat ctcctgcgcg cccgatcagg acgtgcaggg ccccagattc gtccacttgg
     5101 acgggctggt gcccggctcg acctcggcaa cggtgcgctt ggcttgccag aggatgtcgt
     5161 tgtgctgaac ccggtcgccc ttgtcgtaag tcagcgttgc gtcccatgcc ggcgccgtgc
     5221 agccggagcc gctcgccgcg acgatggtga ttctgtcgct ggcggtggcg cctgagttgt
     5281 cgtgggcggt gacggtgacc tcgtgagtgc cggcggggac gttggaccag gtccaggtcc
     5341 aggtatcgaa ctcggaatag acaccagact cgaccccagc cccaacggcg aagacgacgc
     5401 tggcaatcca gccttcggga tcgcccgcca caaccgtgag cgtcacgttc tcgttcgccc
     5461 tgatcgacgt ggatggagaa gccgtgatgc tctcgatcac gggaggctga ttgccgcctt
     5521 caccgccgcc accgccgccc ccgccgcagt cgctgcaggt cacgagctcc caatagcgcc
     5581 cgccggtcgg caggcgcacc cgggagccca cgtgattgcg cttggcctgg tagatgcagc
     5641 cgtcgtagaa gacttcgtcg cccggctggt agctggcgcc cacctgccac agcgccatgt
     5701 ctgcttcgga gtagacgttc agtaataccg aggtcgaact ggtcgagcta cccgcccagt
     5761 ccgaggcgac cgccgtaatc tcgtgaaccc cggcgggcag gttgaaccag gcccagctcc
     5821 agggcggaaa attgtcaaaa cccagggaaa ccccgttgcc gaagaactcc acctgatcca
     5881 ccttggcggg cgccgacacg gtcgtctcag cgccgaggac ctgattgaac ccctccacgg
     5941 agatggcgca actcgccggc tgcgtcactt cgacggcccc gtccaaggca gaactcgaag
     6001 attggactgg agccccgggg tcaccggttc gcatcgcgct taccagggtc ccatcttcgt
     6061 catcgccgct cacttcccag gccatcaccc caccaagccc gaaggcatcc gcgtagcgcc
     6121 gcttgagcgc aatcgactga ggctcgtcca gggtccagaa ggtccgctcc acggggctgt
     6181 aaagccagga ggccagcgcc agcggatccc agtgcagggt gtagtcaccg agcatcagtg
     6241 gtgccaggtc gcggtagtag ttgtagccgt cctcgtagat gccgggcgcc gtccgtccgc
     6301 tctggtaaag gccgtcgtcc gtcgtgctta ccccgctcca gccgcggccg tagaaggcag
     6361 cgccgatcac gagcttctcc acggaaacgc cgtaggtgtc gcggtaaagg cgcacggcgt
     6421 tgtcaatcga cagcttgaac gcatccgagg aggggtcgtt cacggaggtc aggatattgg
     6481 tctggtgacc cgtcttgttc tcccaggtgc cgtgaaagtc gtaggtcatc aggttgaacc
     6541 agtccacgtg cgcctgatcg gcgttgatct ggtagttgtc gccgcggaac gcgctgcccg
     6601 gggtggccat ggtgagcagg tatgggtccg tgccagcagc gcgaggcaaa gcatcgagct
     6661 gccggcggaa ctcggcaagg gtcgcggtga ggttcacgtc gtcgtcggcg ctgtggtggg
     6721 tcccgcccgc cccgccggtg atggggtact cccagtcgat gtcgaagccg tcgaagacgc
     6781 cggccgccac gccgacaccg cccgcgttgc cggcctcggg caggttgccg cggatgaaga
     6841 ggtcgatgca cgacgccacg aacgtctcgc gggacttggg cgttgccgcc gcatccgacc
     6901 accacaccga gccgagccac ccgccgatgg cgatcagcac cttaagatgc ggatgctcgg
     6961 ccttgagctt tttgaactgg ttgaagtggc cgcgcaacgg ttggccggca tcgtcaacat
     7021 cggggatgcc gtcgacgctc atcgccgcgg tgtagagctg ctgataagcc gccacgggat
     7081 cgtccatctg gcagacgacg tcgccggccg cgttgggtgc cggcacgacg aaggcgtagt
     7141 tgatgacggt gagctgatcg
//
LOCUS       DBNHLM010000096        15566 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_731798, whole genome shotgun sequence.
ACCESSION   DBNHLM010000096 DBNHLM010000000
VERSION     DBNHLM010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 15566)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15566)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15566
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_731798"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="AC16QF_04525"
     CDS             <1..706
                     /locus_tag="AC16QF_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6447849.1"
                     /translation="ARGHGDSGTRPIPEGYQWDFFASDLTQVARQLLEETGQPSVEFG
                     IGSSMGGIVTAASEARNPGLFQRIAMLDPPLHPLPELRARLGLGVAAPNADLVAMTRK
                     RKTIWPSRAIARQAWQDKPLFREWVPAAFDAYLEEGLGDRPDGTVALKCDPGVEAAIF
                     ETTGSMDTFELAPRVSAPVLLVRALQGRAPGAAFEHLAGLLPRCTYREVDAGHLLPME
                     SPELTLALLAEFTEGG"
     gene            complement(737..2890)
                     /locus_tag="AC16QF_04530"
     CDS             complement(737..2890)
                     /locus_tag="AC16QF_04530"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HIR6447850.1"
                     /translation="MNTSASDAAARSLAVIEETRQHGSIGHLPVWENITAAHVEAGVR
                     ALLDESEEAFTALETDVEASWAGLMEPLERLQTRIGQVIGAVTHLISVKYSDELQAAY
                     DAIRPIYVSLLNRMSQSLGVYGGLVALRDGPDWPALSQARQRIVTESIRAMERSGVHL
                     EGPPKERFKEIQERLSELGNDFSVNLVKEEKASRVRVQAPEEVAGVPAAVLGLAAAKA
                     REDGEDGATDASGPWHFVVNGVNFVAVMEHALDRGLRERFYRAFRARGTSRDFDNRCV
                     LTEILRLRQEQSALVGFECYADRSLDAKMAPDTDAVWQLFNELEAAARPAAEREFETL
                     KAYMRDEGGLDEGEQPAPWDLAFWSEKMRESLYAYDGERLREYFQLPRVLDGLFSLVG
                     RLYEVEVRRVAAGAVPVWDPAVEFFEVIGAGEVIAGFYVDPYARPGEKRGGAWMNTVV
                     SRSRVLAAPGKSSSLPVALFVMNARPPAEGKVALLSLDEVRTLFHEFGHATQHMFTTI
                     EDGGASGMNLVEWDAVELASQFNEYWMEHKPFLRDLTAHFDTGEPLDDETLDRIVDSR
                     NFMVGTATLRQLFFGKVDMTLHQRFGVAGSNDGASPFEIERDIADSTLVTPRLDDEST
                     LPAFGHLFAGGYAAGYYSYKWAEVLAADAFAAFKEAGLDDGAAMKEIAGRFRDTVLGL
                     GGSLPAGDVYRRFRGRDASVRALLADQGLVAAEAG"
     gene            complement(3018..3896)
                     /locus_tag="AC16QF_04535"
     CDS             complement(3018..3896)
                     /locus_tag="AC16QF_04535"
                     /inference="COORDINATES: protein motif:HMM:NF014045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR6447851.1"
                     /translation="MARENEDRNLALFCDFENVALGVRDAKYARFDIQLVLERLLVKG
                     SIVMKKAYCDWDRYKEFKPVMHEAAFELIEIPHTRQSGKNSADIRMVVDALDLCYTKE
                     HIDTFVIISGDSDFSPLVSKLRENAKTVIGIGVKNSTSDLLIGNCDEFIFYDDLVRHQ
                     KATRKPRKKPAAKKAPEKKDATDATDAMDGKDAKEAKRAEPARSEEGNTDEGIALVLE
                     TAEALYGERGEREKLWGSMVKQTLKRRRPGFNESFYGFRSFSDLLEEAESRGLLELEM
                     DERSGGYLIRRLTREE"
     gene            4192..5733
                     /locus_tag="AC16QF_04540"
     CDS             4192..5733
                     /locus_tag="AC16QF_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HIR6447852.1"
                     /translation="MLEDLLSKLPLEPVPVIVCAGIAVGCLLLGWICKAIGANRKLGQ
                     LRKEALEAKRAVPQLESSVHNRDLQIARFKLEAEDLTARNEELSTQLSTTNKELRSAR
                     REARNLNTELKAIKGHQSSLDNEVIAGFEEEDPLASADDQLKARMGRVESLYERVKEA
                     VIEREDRIQKLEEQLAQARNGNAAGGALLDDSDRDWTESTQELTEKVSSQEGLIESLR
                     AQIRKMQTEKEMLADMAKRRSDSNRALKEASAEAEAHVPVLRARLEERDRAIAENEAA
                     ISVLRDEVDMLRAQNAALEFESSGNVAADQPPVEDIFAIRKLLEDEFEAREEAFEKRL
                     REAEQAKEIAEADLALALAEQSKRVEEATSRIESLEAELGERRESTDVFKSVAEDREL
                     KLATLTNRVRVLLAAVNGDTATEHERLVELIRQLDPGHVQGPVPESSGTSDANGPARA
                     VKQAPPLPMRRAPRRPLRVIETRPESPPAPRGSRRAEVGRVLSALRPARSSVVPMRRR
                     GRMRR"
     gene            complement(5730..6227)
                     /locus_tag="AC16QF_04545"
     CDS             complement(5730..6227)
                     /locus_tag="AC16QF_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6447853.1"
                     /translation="MTVEVNGIAHIQLTVRDPARCVPFWERLCHFLEMSTLIRAEEIV
                     YCIGSRTGVLVRGAPEDKRDRAFDQDRPGLHHLCFRARSREDVDAIYEFVAGELDAHV
                     VHPPEDGSHFAPGYYSVLFEDPDGIRVEVNFVPGRGHFGSEGRLGPGGRGPASRYGEE
                     GLGDG"
     gene            complement(6255..7196)
                     /locus_tag="AC16QF_04550"
     CDS             complement(6255..7196)
                     /locus_tag="AC16QF_04550"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR6447854.1"
                     /translation="MMNNTRLLVALAWLSLAPAGWAEDECTPSRWGSDDEIGNVNLIT
                     TQSVLAASRLIKTGKTYSLGIVVDGNTPAFPPRSLSLQIVQPGQQEGNRPLSRMTYND
                     DVFQGWFGIGSQLDGLGHAGDEGMYYNCNHARDFSDIAGLTKLGIEKVPPIVARGIVL
                     DMTAHFGVEHLAGGQTFSVDDVRAVEKKQGTPVREGDVVLFHTGWTDAMLETDPAGWS
                     ASAPGISKAVAGYLAGRNVVAVGSDTWGLDVLPPEREDEPFISHLILLQQNGIYILET
                     MNTGALVRDEAHEFLFVLGQAKVRGAVQMFINPIAIR"
     gene            complement(7376..8065)
                     /locus_tag="AC16QF_04555"
     CDS             complement(7376..8065)
                     /locus_tag="AC16QF_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HIR6447855.1"
                     /translation="MPEFQGFPPRTLKFLKALERNNDRDWFRENKALYERDVLDPSLD
                     FIATMGPKLAAISDHFVAIPKRTGGSLMRVYRDTRFSKDKTPYKTNIGIQFRHELGKD
                     VHAPGYYLHIDTAGCFVAAGMWRPDAPSLARVRELIAERPGDWQKARDARPFKAAFEL
                     GGESLKRPPKGFAPDAPHLQDLRRKDFIASANVATGELGQPQLVDVVAELYARATPFM
                     RFLCRAIEVEF"
     gene            complement(8128..9405)
                     /locus_tag="AC16QF_04560"
     CDS             complement(8128..9405)
                     /locus_tag="AC16QF_04560"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6447856.1"
                     /translation="MAGVWQELDRIVRPQGQVFYGWWIVLASSGVQWLAAVLWMQSYG
                     AYMVLLQQDFGWSKAMVAGAFALTRIESGILGPLQGWLVDRFGPRAILTIGTVLFGVG
                     FILFGQVESLLTFYLTFALIALGASLGGFATLMVAIVNWFSRHRSKAIAVSQLGFSLG
                     GLSVPLIIWALEAFGWRATAVVSGLLVLVVGLPLVQVVRHRPEAQGELADGHAASGGG
                     PEGRFSLGGDFTARQAMRTRSFWLISFGHALALLTVSAMMVHLVPHLTEGMGYSLSSA
                     GMVVAVLTAFQLLGQLSGGYLGDLFNKRLICGVCMFAHAGGLLLVTYADSIVGVLAFT
                     LLHGLAWGTRGPLMVSLRADYFGPTSFGTIMGFSSLIVMLGMSGGPIIAGIMADVSGD
                     YHSGFTLLASLSFMGSLCFFAATPPSPPARASA"
     gene            complement(9476..10150)
                     /locus_tag="AC16QF_04565"
     CDS             complement(9476..10150)
                     /locus_tag="AC16QF_04565"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR6447857.1"
                     /translation="MAGGSSERGDERDALVALIESEVADTADYTGRSKLRPAVLEAMR
                     AVPREEFVPPEEAYAAYANVPLPIGHFQTISQPYVVALMTDLLDPEPGDVMLEVGTGS
                     GYQAAVLSTLVSRVYSLEVVPQLAASAAERLARLGYDNVVTREGDGNLGWPDHAPYDG
                     IVVTAGAPEVPPALIDQLKPGAALVIPVDAGFNQMLRRITRDPDGDVHTEDVLPVAFV
                     PLVKAT"
     gene            10342..11169
                     /locus_tag="AC16QF_04570"
     CDS             10342..11169
                     /locus_tag="AC16QF_04570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6447858.1"
                     /translation="MPKDNADQIEFWNGPTASNWVAAEERMDELLAPITTAILQAAAV
                     SPGEAVLDVGCGCGATSRAMAAVGARVTGVDVSAPMLARARQRADDESLSFIEADAQS
                     HPFDPGFDLLFSRFGVMFFADPVAAFANLRGALNPGGRLCFICWRAPGENPWLSIGMA
                     AAQPFLPPAETPDPRAPGPFAFADPDYLASVLADAGFEDVAITPHNPEMRLGADLTEA
                     MEFVTGVGPLSNVLKELAADLREAALAAVREALAPFAGPSGVLLGGACWIAQGRRGA"
     gene            complement(11195..12385)
                     /locus_tag="AC16QF_04575"
     CDS             complement(11195..12385)
                     /locus_tag="AC16QF_04575"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR6447859.1"
                     /translation="MQTFVTHLECSLTGEHYPADQVHGLSAAGRPLLVRYDLTGLSRA
                     VGKADIAGRQGGFWRYREFLPVRRTENVVSLGEVVTPMIPIAGPAGGSVWVKDEGRLP
                     TGSFKARGLALAVAMARELGLSHLAMPTNGNAGAALAAYAARAGLRATVLCPSDTPEI
                     NVREIAMQGAEVWRVDGLINDCGRIVGEAAGRTGWFDLSTLKEPYRIEGKKTMGIELA
                     EQLGWRLPKAIFYPTGGGTGLIGMWKAFAELEAIGWIGPERPQMFAVQAEGCAPIVRA
                     FERGDEHAELWENAHTAAAGIRVPAAVGDFLILRAVRESGGAALAVSDADLLAAQRRI
                     AAQEGLLLCPEGAATYVAWRQALEAGLVGAEDEVVLFNCATGLKYPMPDESRTFTRLD
                     DLIG"
     gene            complement(12439..12777)
                     /locus_tag="AC16QF_04580"
     CDS             complement(12439..12777)
                     /locus_tag="AC16QF_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family probable chaperone protein"
                     /protein_id="HIR6447860.1"
                     /translation="MNMKPIFRQIVFMLALVFSAVAVAAMGLDAAKAQGLVGEQHDGY
                     LGIVSSATPEVRALVSDVNAKRRAEYQRIAQTNGIDVKDVELLAGQKAIQRTAPGHYV
                     KLEGQSWRPK"
     gene            complement(12787..12969)
                     /locus_tag="AC16QF_04585"
     CDS             complement(12787..12969)
                     /locus_tag="AC16QF_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HIR6447861.1"
                     /translation="MLLALALALAVLAAAGCTPTVKVATDEPITINLNVNIKHEILVR
                     VDKELNDLFGEDSDLF"
     gene            complement(13114..15270)
                     /locus_tag="AC16QF_04590"
     CDS             complement(13114..15270)
                     /locus_tag="AC16QF_04590"
                     /inference="COORDINATES: protein motif:HMM:NF023167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane phospholipid transport protein
                     YdbH family protein"
                     /protein_id="HIR6447862.1"
                     /translation="MKWLLAIVILLAGLLAGAYASLPWLAETLAVREAAAVGIEVREL
                     DVRFPRWGEIAVDRLVASMQGNELRAEQLTVGYNWSGVRERRLAFVGAALLTVYVEDS
                     SGDAEPGALGEQIVVLPGLFLDAWQELPVESVQVDEVRLRTGELVFAGTFGFTPAAAS
                     LAGEVSGVEPLGALRLSMRADAAGSVGLAIQQLERPANSLAVSADARRLAGEAVIAWN
                     LELPVEDGELAVTLEGRGPIELSENGWRLGSGLQARVDGGAWRGGFAVAEAEGPWPED
                     EATELVRVSGAWQLEADLPHDVAARGTLSAGLDRSGAKLSIDPGMVVTGAAVSGASWS
                     LEGIRLDTLDGLALEVGIEPMTLTLGSPVRAALAADAARWENFALAPYVDLRIDSLAM
                     DPGSARGAFAATVPALQLSASGQIRADAESSTMVVELSARQRLRAPLLATFLAGWDQP
                     YDVDAGNVAVTGSLTLGDGALSGDVVVTLEGVQSHYGEVVAAGIAGEARVKLREDGWQ
                     ATADTLSVAVADPGFPLRDLRFAAELTPATALLSAVRGRALGGELFIDRLAYDMNDAT
                     AAFLVIATGIELADVLALEGESVTGSGILDGELPVELLEGGTRITNGRFLARAPGGVL
                     RYPDAGKAAEALGQQVGGFALAPLVDFRFEVLDAHVDLQPSGDLLLGVRLEGRSPEFE
                     RPYHYNLNISENIPALLKSLRISEEFQRRLEEVLQR"
     gene            complement(15271..>15566)
                     /locus_tag="AC16QF_04595"
     CDS             complement(15271..>15566)
                     /locus_tag="AC16QF_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447863.1"
                     /translation="LYLLLLPATYGLLGVGMVASSTFIALGKPMPTLVLSLLRMIVLY
                     IPMALIMETLFGYAGIFAAAAVCNCLFGGLAYGWVRRMLHKEISRHGRYEGAG"
BASE COUNT         2850 a         5310 c         4958 g         2448 t
ORIGIN      
        1 tgcccggggc cacggcgact cgggaacacg ccccatcccc gaaggatacc agtgggactt
       61 cttcgcctcg gatctcaccc aggtggccag gcaactgctc gaggaaacag ggcagccatc
      121 cgtcgagttc ggcatcggca gctccatggg cggcatcgtc accgccgcct cggaagcacg
      181 caacccgggg ctcttccagc gcatagccat gctggacccg ccgctacatc cgctacccga
      241 gctgcgcgcc aggctcgggc tcggcgtcgc ggcgccaaac gcggacctgg tggcaatgac
      301 gagaaagaga aagacgatct ggccgtcccg cgcgatcgcc cggcaggcgt ggcaggacaa
      361 gccgcttttc cgcgaatggg taccggccgc attcgacgcc tacctcgaag aggggctcgg
      421 cgaccgcccg gatgggacgg tggcgctgaa gtgcgacccc ggcgtggaag ccgctatctt
      481 cgaaaccacg ggttctatgg acaccttcga gctcgccccg cgtgtgagcg cgcccgtgct
      541 gctcgtccgc gccctgcagg gccgggcccc cggagccgcc ttcgagcacc tcgcaggcct
      601 tctgccgcgc tgcacctaca gggaggtgga cgccggacac ctgctgccca tggaatctcc
      661 cgagctgaca ctggcgctgc ttgccgagtt caccgagggc ggctgagccg tcggcaaccg
      721 cttaggctcg ggcgcgtcag ccagcctcgg ccgcgaccag gccctgatcg gccagcagcg
      781 cacgtaccga ggcgtcccgc ccccggaaac gccggtagac gtcccccgcc gggaggcttc
      841 caccaagacc gaggacggtg tcccgaaagc gcccggcaat ttctttcatc gccgcgccgt
      901 catcgagccc cgcctccttg aaggccgcaa aggcatccgc cgcaaggacc tcggcccact
      961 tataggagta gtagccggcc gcgtagccac cggcgaagag atgaccgaag gcaggcagcg
     1021 tactctcgtc gtcgagcctc ggcgtgacga gggtgctgtc ggcgatgtcc cgctcgatct
     1081 cgaaggggct cgcgccgtcg ttgctgcccg cgacgccgaa tctctggtgc agggtcatat
     1141 cgaccttgcc gaaaaagagc tgccgcaggg tggccgtccc caccatgaag ttgcggctgt
     1201 cgacgatccg atcgagcgtc tcgtcgtcca gcggctcccc ggtatcgaag tgggcggtga
     1261 gatcgcgaag aaagggtttg tgctccatcc agtactcgtt gaactggctg gcgagctcca
     1321 cggcgtccca ctcgacgagg ttcatgcccg atgcgccgcc atcctcgatg gtcgtgaaca
     1381 tgtgctgggt ggcgtggccg aactcgtgaa agagcgtgcg cacctcgtcc agcgacagca
     1441 acgccacctt gccctctgcc ggggggcgcg cgttcatgac aaacagggcc acgggcagcg
     1501 acgacgactt accaggcgcg gcgagcaccc ggctgcgcga caccacggtg ttcatccacg
     1561 cgccgccgcg cttctcgcca ggccgggcgt agggatccac gtaaaagcca gcgatgacct
     1621 cgccggcccc gatcacctcg aagaactcga ccgcgggatc ccagacgggc accgcaccgg
     1681 ccgccacgcg acgcacctcg acctcgtata gccgaccaac cagggaaaac aggccgtcga
     1741 gaacgcgggg aagctgaaaa tactcgcgca ggcgctcacc gtcgtaagcg tagagcgact
     1801 cgcgcatctt ctcggaccag aatgccagat cccagggcgc gggctgctcc ccttcgtcga
     1861 gaccgccctc atcgcgcatg tacgccttga gcgtttcgaa ctcgcgctcc gcagcgggcc
     1921 gcgcggctgc ctccagctcg ttgaaaagct gccagacggc gtccgtgtcc ggtgccatct
     1981 tggcgtccag actgcggtcg gcatagcact cgaaccccac gagggcggac tgctcctggc
     2041 gaagccggag gatttccgtg agcacgcacc ggttgtcgaa gtccctggat gtgccacggg
     2101 cgcgaaacgc ccggtagaac cgctcacgca gcccccgatc cagcgcgtgc tccatgacgg
     2161 cgacgaagtt cacgccgttg acgacgaagt gccacggacc ggaggcgtcc gtagcgccgt
     2221 cctccccgtc ttcccgggcc ttggcggctg ccagcccgag gacggccgcg ggcactccgg
     2281 caacctcttc aggcgcctgc actcggaccc gcgacgcttt ctcctctttg accaggttca
     2341 cggagaaatc gttgccgagc tcggacaggc gctcctggat ctccttgaag cgctccttgg
     2401 ggggcccttc cagatgcacg cccgaacgtt ccatggccct gatggactcc gtgacgattc
     2461 gctgccgagc ctggctcagt gccggccagt cggggccgtc ccgcaacgcg accagacccc
     2521 cgtatacgcc caggctctgg ctcatccgat tgagcagcga cacgtagatg gggcgaatcg
     2581 cgtcgtaggc cgcctggagc tcgtcggagt acttgacgct gatcaggtgg gtcaccgcgc
     2641 cgatcacctg accgatgcgc gtctgcaggc gttccagggg ctccatgagc ccggcccagg
     2701 acgcctcgac gtcggtttcc aatgcggtga aagcttcttc gctctcatcg agcagggcgc
     2761 gaacgccggc ctccacatga gccgccgtga tgttctccca tacgggcagg tgcccgatgg
     2821 atccgtgctg ccgggtttcc tcgatgacgg ccaggctgcg ggcagccgcg tcagatgccg
     2881 atgtgttcac gatgatctcc ttggcaagcc tctcggggcc ggcgtgcttt gagcagcgag
     2941 gtttgtccac acgatgaggc ggtttctccc ctcgtccgtc cggcgggtcc cggcgagggg
     3001 aagcgcattc taaggcgtca ttcttcccgg gtcagccggc ggatcaggta tccgccggag
     3061 cgttcgtcca tttccagttc cagcaggccc cggctttccg cttcctcgag gagatcgctg
     3121 aacgagcgga agccgtagaa actctcgttg aaccccgggc gccgccgctt gagcgtctgc
     3181 ttcaccatgg agccccagag tttttcccgc tcgccacgct cgccgtacag cgcttcagcg
     3241 gtctccagca ccagcgcgat cccctcatcg gtgttgccct cttccgagcg ggcgggctcg
     3301 gccctcttcg cctctttggc gtccttgccg tccatggcgt ccgtggcgtc cgtggcgtcc
     3361 tttttctcag gcgccttctt tgcggccggc ttcttgcgtg gcttgcgggt cgccttctga
     3421 tggcgaacca gatcgtcgta aaagatgaac tcgtcgcagt tgccgatcag gaggtccgac
     3481 gtcgagttct tcacgccgat cccgatgacg gtcttcgcat tctccctgag cttgctgacc
     3541 agcggcgaga aatcggagtc gccgctgatg atcacgaacg tatcgatgtg ttccttggtg
     3601 tagcagagat ccagggcgtc cacgaccatt ctgatgtccg ccgagttttt acccgactgg
     3661 cgcgtgtggg gaatctcgat cagctcgaac gccgcctcgt gcatgacggg cttgaactcc
     3721 ttgtagcgat cccagtcgca ataggctttc ttcatcacga tgctgccctt gaccagcagg
     3781 cgctcgagca ccagctggat gtcgaacctg gcgtacttcg catcacgcac gccgagggca
     3841 acgttctcga agtcacagaa gagcgcaagg tttcggtctt cgttttcgcg ggccatgagg
     3901 tatgccgcag ctgaggttgc gcctacgtta acggggccgc gtgcggccgt ccacaagcaa
     3961 acggtcacgc aaggcggcga ccaggggatc gcagggctca gatcgacgca agcggaagtg
     4021 acagcgcctc cccgacccga cggggccgcc cccactgggc gtgatcgggt tcacgatttc
     4081 ggggcacaag cgtgcggatc ggcgagggcg ccggtgcctg ctgagacaca ggtaactgaa
     4141 tggcccaacc attgcgggaa ccattacgca ggaccatgtt tggctgatca catgctcgaa
     4201 gatcttttgt ccaagctgcc tcttgagccc gtgccggtca tcgtctgcgc gggcatcgcc
     4261 gttggctgcc tgctgctcgg atggatctgc aaagccatcg gcgccaatcg caagctgggt
     4321 caactgcgca aggaggccct cgaggccaag cgtgccgtgc cccagctcga gtcttccgta
     4381 cacaatcgcg atctgcagat tgcccggttc aagcttgaag ccgaggatct gaccgctcgc
     4441 aacgaggagc tgtccactca gctctcaaca accaacaagg agctgcgcag cgctcgccga
     4501 gaagcccgaa acctgaatac cgagctcaag gcgatcaagg gccatcagag cagcctggac
     4561 aatgaagtca tcgccggctt cgaggaagag gatccgctcg ccagcgccga cgaccagctc
     4621 aaggcccgaa tgggccgcgt cgagtcgctg tacgagcgcg tcaaggaagc ggtgatcgaa
     4681 cgcgaagatc gcattcagaa gctcgaggag cagctggctc aagcgcgcaa cggaaacgcg
     4741 gcaggcggcg ccttgctgga tgactccgat cgggattgga cagaatcgac ccaggaacta
     4801 accgagaagg tcagctccca ggaagggctg atcgaatccc tgcgagccca gatccgcaaa
     4861 atgcagaccg agaaggaaat gctcgctgac atggcaaagc gccgcagcga tagcaaccgg
     4921 gccctgaaag aggccagcgc cgaggccgaa gcccatgtgc ccgtgctgcg cgcccgcctc
     4981 gaagagcgcg acagagccat cgcggagaac gaggcggcca tcagcgtgct gcgcgacgag
     5041 gtggacatgc tgcgcgccca aaacgcggcc ctggagttcg agagttccgg aaacgtcgca
     5101 gcagaccagc ctccggtgga ggacatattc gccattcgca agctgctcga ggacgagttc
     5161 gaagcccgcg aggaggcctt cgagaaaagg ctgcgcgagg cggagcaggc caaagagatc
     5221 gccgaggcag atcttgccct cgcgctcgcc gagcagagca agcgggttga ggaggcgact
     5281 tcccgcatcg agagcctgga agccgagctc ggcgagcgcc gcgaatccac ggacgtgttc
     5341 aagagcgtgg ccgaagacag agagctcaag cttgccacgc tgaccaacag ggtgcgcgtg
     5401 ctgctcgccg ccgtgaacgg cgataccgca accgagcacg agcggctggt ggagctcatc
     5461 aggcagctcg atcccggcca cgtgcaggga ccggtaccgg aaagctccgg cacttccgat
     5521 gccaacggac ctgcccgggc tgtgaagcag gcgcccccgc tgcccatgcg ccgagcgccc
     5581 aggcgaccgc tgcgggtgat cgaaacccgt cccgagagcc ccccggcacc gcgcggctcc
     5641 cgccgagccg aggttggccg ggtgctgagc gcgttgcgtc cggctcggtc gagcgtcgtg
     5701 ccgatgcggc gacgtggccg catgcgccgc tagccgtctc ccagaccctc ttccccatag
     5761 cggctcgccg gtccccggcc gccgggaccc agacgcccct ctgacccgaa gtgcccgcgg
     5821 cctggcacga agttgacctc cacccgaata ccgtcgggat cctcgaacaa cacggagtag
     5881 tatccgggag caaagtgaga gccgtcctcg ggaggatgca cgacgtgcgc gtccagctcg
     5941 ccggcaacga actcgtagat ggcgtcaacg tcctcccggg agcgggcccg gaaacacaga
     6001 tgatgaaggc ccgggcgatc ctggtcgaat gcccggtcgc gcttgtcctc gggggcgccc
     6061 ctcacgagga cgcccgtgcg gctgccgatg cagtagacga tctcttccgc ccggatgagc
     6121 gtgctcatct ccagaaagtg acacaaccgc tcccagaacg gcacgcagcg cgctggatcc
     6181 cgaaccgtca gctggatgtg cgcgatcccg ttgacctcca ccgtcatcaa gctcctcctt
     6241 cgcgaccgcc gtcgtcagcg aatggcgatg gggttgatga acatctggac cgcaccccgg
     6301 accttcgcct ggccgagcac aaagagaaac tcgtgggctt cgtcgcggac gagagcgccc
     6361 gtgttcatgg tctcgagaat gtagatcccg ttctgctgca gaaggatcag gtggctgatg
     6421 aagggttcgt cctcgcgctc gggcggcaga acgtcgagcc cccacgtgtc agacccgacg
     6481 gcaaccacgt tcctgccggc gaggtagccg gcaaccgctt tggaaattcc cggggcggag
     6541 gccgaccagc cggctgggtc cgtctcgagc atggcgtccg tccaccccgt atgaaacagc
     6601 acgacgtcac cctcgcgaac gggtgtgccc tgcttcttct ccaccgcgcg gacgtcgtcc
     6661 acgctgaacg tctgtccgcc agccagatgc tcgacgccga aatgggccgt catgtccagc
     6721 acgatgcccc gggcaacgat gggcggaacc ttctcgattc ccaacttggt caacccggcg
     6781 atgtcggaga agtcccgggc atggttgcag ttgtagtaca taccctcgtc accggcgtgg
     6841 cccaggccgt cgagctggga accgataccg aaccagccct ggaacacgtc gtcgttgtag
     6901 gtcatgcgcg acagaggccg gttaccctcc tgctgcccgg gctgaacgat ctgcagcgac
     6961 aacgaccgcg gcgggaacgc cggcgtgttg ccgtccacca cgatgccgag gctgtaggtc
     7021 ttccccgtct tgatgagccg ggacgccgcc agcaccgact gggtcgtgat gagattgacg
     7081 ttgccgatct cgtcatcgct cccccagcgc gagggcgtgc attcgtcttc cgcccacccc
     7141 gccggagcca ggctcagcca tgccagcgcc accagcaatc gcgtgttgtt catcatcccc
     7201 cctttcgttg accggcctga aatcccacgc tgcgcaccgg ctcggctgac accgccaccg
     7261 ccccggagca tcggccacct tagcatccgg taaagatccc ggcgatcgat tccgcagagc
     7321 ggatcgggag agcccggcgg cgaccgccgg gccgccaagc catcgggcgt aggcgtcaga
     7381 actcgacctc tatggcccgg cacaggaagc gcatgaaggg cgtcgccctc gcgtaaagct
     7441 ccgccacgac atcgacaagc tgcggctggc caagctcgcc ggttgcaacg ttcgcgctgg
     7501 caatgaagtc cttgcgcctg agatcctgca gatggggcgc gtccggcgcg aagcctttcg
     7561 gcggccgctt cagcgactcg ccccctagct cgaatgccgc cttgaagggg cgggcgtcac
     7621 gcgccttctg ccagtccccg ggccgctcgg cgatgagctc ccgcacgcgg gcaagcgacg
     7681 gcgcatcagg acgccacatg cccgcggcaa cgaagcatcc ggcggtgtcg atgtgcaggt
     7741 agtaaccggg cgcgtgaacg tctttgccga gctcgtgacg gaactggatc ccgatgttgg
     7801 tcttatacgg cgtcttgtct ttagagaagc gggtatcccg atagacgcgc atgagcgacc
     7861 cgcccgtccg cttggggatc gccacgaaat ggtcggatat ggcggccagc ttggggccca
     7921 tggtggcgat gaaatccagc gacgggtcaa gaacgtccct ctcgtagagc gccttgttct
     7981 cccggaacca gtcccggtcg ttgttgcgtt ccagggcctt cagaaacttc aacgtccggg
     8041 gcggaaaacc ctgaaactca ggcattggcc cgctcctcac caatcatcga gaaaggatgg
     8101 gagcataccc agcttccggc ccgagcgtca tgccgatgcg cgtgcaggcg ggctgggagg
     8161 cgtggcggca aagaagcaca gcgaacccat gaacgacagg ctggccagca gggtgaaccc
     8221 gctgtgatag tcgccggaaa cgtcggccat gatgcccgcg atgatcggcc cgcccgacat
     8281 tcccagcatc acgatcagag acgagaagcc catgatggtg ccgaaagagg tgggaccgaa
     8341 ataatcggcc cgcagcgaga ccatgagggg ccctcgggtg ccccaggcaa ggccgtggag
     8401 cagcgtgaac gccagcacgc ccactatgga gtccgcgtag gtgacgagca ggagcccgcc
     8461 ggcatgagcg aacatgcaga ccccacagat caggcgcttg ttgaacagat cgccgagata
     8521 gccgccggag agctgaccga gaagctgaaa ggcggtgagc acggctacca ccatgcccgc
     8581 ggaggacagc gagtagccca tgccctcggt cagatgaggc accaggtgaa ccatcatggc
     8641 cgagaccgtg agcagggcca gcgcgtgacc gaaggagatc agccagaacg agcgcgtccg
     8701 catggcttgt ctggccgtga agtcgccgcc cagggagaag cgtccctccg gaccgccgcc
     8761 ggaggccgcg tgcccgtctg ccagctcccc ctgagcctcc ggtcgatggc gcaccacctg
     8821 taccagtgga aggccgacga cgagcaccag caggccggac accacggcgg tggcgcgcca
     8881 gccgaacgcc tccagcgccc agatgatcag cggcaccgag agcccgccga gggagaagcc
     8941 gagctgggat accgcgatgg ccttcgaccg gtggcgactg aaccagttga cgatggccac
     9001 catcaacgtg gcgaaacctc ccaggctcgc gccgagcgcg atgagcgcga acgtcagata
     9061 gaaggtcagc agggactcca cctggccgaa caggatgaac ccgaccccga agagcaccgt
     9121 gccgatggtg aggatcgcgc ggggaccgaa tcggtccacc agccagccct ggagcggtcc
     9181 gaggatgccg ctctcgatcc gggtcagggc aaacgccccc gcaaccatgg ctttgctcca
     9241 cccgaaatcc tgctgaagga gcaccatgta agccccgtag gactgcatcc aaagcacggc
     9301 ggccagccat tggacaccgc tgctggccag cacgatccac cagccataga aaacctgtcc
     9361 ctgaggccgc acgatgcggt caagttcctg ccagacgcct gccatgggaa aagatgatac
     9421 gccattggtc ggccatccat gacgtcgcag gaagctaagg ttgcgcggac ccctcctagg
     9481 tcgctttcac gagcggcacg aatgccaccg gcaggacgtc ctccgtgtgg acgtcgccgt
     9541 cgggatcgcg ggtaatccgc ctgagcatct ggttgaaccc ggcgtccacc ggaatcacga
     9601 gggccgcgcc gggcttgagc tgatcgatca atgccggcgg aacttccgga gcaccggcgg
     9661 tcaccacgat gccgtcgtag ggcgcgtggt ccggccagcc cagattgcca tcgccctccc
     9721 gggtgaccac gttgtcgtat cccagccggg ccagccgctc ggcggcgctg gcggcgagct
     9781 ggggaaccac ctccagggag tagacgcggc tgaccagggt gctcagcacc gccgcctggt
     9841 atccgctccc cgtgccgact tccagcatca cgtcgccggg ctccggatcc agcagatcgg
     9901 tcatgagggc gacgacgtat ggctgcgaga tcgtctggaa gtgtccgatg ggcagcggca
     9961 cgttggcgta ggcggcgtag gcttcctccg gaggcacgaa ctcttcccgc ggcacggcgc
    10021 gcatggcttc gagcacggcg ggtcgcagct tcgagcggcc cgtgtaatcg gcggtatccg
    10081 ccacttcact ctcgatcagc gcaacgagcg cgtcgcgctc gtccccacgt tcggaagatc
    10141 cccctgccat cgcttctgat caccccgccg gcatcgccct tgcctcgagt ttacccgagc
    10201 ccgcgcaccg ggccattgag ggactccgaa aaaaggcggg catccctggg cagggtgacc
    10261 gggcggcggc gtgcttctag aatctctctt cgccgtgcgg tttagccccg cacccaccgg
    10321 aaacacactg acgaaataga catgccaaaa gacaacgccg atcagatcga attctggaac
    10381 gggcccacag ccagcaactg ggtggccgca gaggaacgca tggacgagct gctggccccc
    10441 attaccaccg ccatactcca ggccgcggcc gtgagccccg gcgaggccgt cctggacgtc
    10501 gggtgcggtt gcggcgccac gtcgagggcc atggcagcgg tcggagctcg ggtgaccggt
    10561 gtggacgtat ccgcgcccat gctcgcccgg gcacgacaac gcgccgacga cgagtccttg
    10621 agcttcatcg aagcggatgc ccagagccac cccttcgatc ccggtttcga tctcctgttc
    10681 tcccggttcg gggtgatgtt cttcgccgat ccggtagcgg ctttcgccaa cctgcggggc
    10741 gcgctgaacc ccggggggcg cctgtgtttc atctgctggc gggcccctgg ggagaatccc
    10801 tggctgagca tcggcatggc cgccgcgcag cccttcctgc cgccggcgga gacgcccgac
    10861 ccccgagcgc cggggccgtt tgccttcgcc gatccggatt atctggcttc ggtgctggcc
    10921 gatgccgggt tcgaagacgt ggccatcaca ccccacaatc ccgagatgcg cctgggtgca
    10981 gacctgacag aggccatgga gttcgtgaca ggggtcggac cgttgagcaa cgtcctcaag
    11041 gagctggctg cggatctgcg ggaggccgcg ctggccgccg tgcgggaggc tttggcgccc
    11101 ttcgccggcc cctcaggcgt cctgctgggg ggcgcgtgct ggatcgccca agggcgtcgg
    11161 ggcgcctgaa cggcgggcgg gtcgcccgcc gggatcagcc gatgaggtcg tcgagcctgg
    11221 tgaaggtccg gctctcgtcg ggcatgggat acttcagccc cgtggcgcag ttgaagagaa
    11281 cgacctcgtc ctcggcgccg accaggcccg cctccagggc ctggcgccag gcgacgtagg
    11341 ttgcagcgcc ctcggggcag agcagcagac cctcctgcgc cgcaatgcgc cgctgggcag
    11401 cgaggaggtc tgcatcgctc accgcaaggg cagcgccccc ggactcgcgc acggcgcgca
    11461 gaatgagaaa gtcgcccacg gcggcgggca cccggatacc ggctgccgcc gtgtgcgcgt
    11521 tctcccacag ctcggcatgc tcgtcgccgc gctcgaacgc gcgaacgatg ggggcacagc
    11581 cttcggcctg cacggcaaac atctgcggtc gctccggccc gatccagcca atagcctcga
    11641 gctcggcgaa cgctttccac atgccgatca gaccggttcc accgccggtc gggtagaaga
    11701 tggccttggg cagacgccag ccgagctgct cggcgagctc tatgcccatc gtcttcttac
    11761 cctcgatccg gtagggctct ttcagggtgg aaagatcgaa ccaaccggta cgtccggccg
    11821 cctcgcccac gatgcgcccg cagtcgttga tgagcccgtc cacgcgccat acctccgcgc
    11881 cctgcatcgc gatctcgcgc acgttgatct ccggggtatc cgagggacac agcacggtgg
    11941 cccggaggcc ggccctcgcc gcataggccg caagtgccgc cccggcgtta ccgttggtgg
    12001 gcatggccag gtgggaaaga cccagctccc tggccatggc cacggccaga gcgaggcctc
    12061 ttgccttgaa cgaaccggtg ggcagtcggc cctcatcctt gacccagacg ctgccgccgg
    12121 cagggccggc aatcgggatc atgggggtga cgacttctcc cagggagacc acgttctccg
    12181 tgcgacgcac gggcagaaac tcccggtaac gccagaagcc gccctggcgg ccggcgatgt
    12241 ccgccttgcc caccgctctc gaaaggcccg tcaggtcgta tctgacgagc agcggtcgcc
    12301 ccgcagcaga gaggccgtgg acctgatcgg ccgggtagtg ctccccggta agcgagcact
    12361 cgagatgggt aacgaaggtc tgcaaagcgg tttaggactg gggttgggcg agcccgctct
    12421 cgcagccagg ctcgccggct atttcggccg ccagctctga ccttcgagct tgacgtagtg
    12481 accgggggcg gtgcgctgaa tggccttctg gccggcaagc agctccacgt ccttgacgtc
    12541 gatgccgttg gtttgagcga tgcgctgata ctccgcgcgc cgcttggcat tgacatcgga
    12601 gacgagggcc ctgacttcag gcgtggcgga cgagacgata cccaggtaac cgtcgtgctg
    12661 ctctccgacc agaccctgtg cttttgccgc atccaggccc atggccgcga cggccactgc
    12721 cgagaagacc agcgcgagca tgaaaacgat ttgccgaaag attggtttca tattcatcgc
    12781 gtcgccttaa aagagatcac tgtcttcgcc gaacaggtca ttcagctctt tgtccactct
    12841 cacgagaatc tcgtgcttga tgttgacgtt aaggttgatg gtgatgggct catccgttgc
    12901 gaccttcacc gtcggggtgc atcccgcagc ggcgagaacg gcgagagcaa gggcgagcgc
    12961 gagcagcatc cctgaggcgg ccgtacgcgg cctgaccggt gggatccggc ggcaggaaac
    13021 cggcaattcg aacgagttac tcatcatcaa agagtacctg gcgcactgtg aaccggagct
    13081 gaacattacc cagcgccgac accccccgca agctcacctt tgcagaacct cttccaggcg
    13141 tcgctggaac tcttcggaaa tgcgcaggga tttgagcagg gccggaatgt tctccgagat
    13201 gttcaggttg tagtgatagg gccgctcgaa ttccggactg cgcccttcca accgaacccc
    13261 gagcaggagg tcgccgctcg gctggagatc gacatgcgca tcgagcacct cgaatcggaa
    13321 atccaccagc ggcgcgagag cgaacccgcc gacctgctgg ccgagcgctt cggccgcctt
    13381 ccccgcatcc ggatagcgca gaacgccccc gggcgcccga gcgaggaaac gtccgttcgt
    13441 gatcctggtg ccgccctcga ggagctcgac aggcagctcg ccatcgagaa tgcccgatcc
    13501 ggtcaccgac tctccttcca gagccagcac gtcggctagc tcgatcccgg ttgcgatcac
    13561 cagaaaagcg gcggtggcgt cgttcatgtc gtaggccagt cggtcgatga agagctcccc
    13621 gccaagcgct ctgccccgaa ccgcgctgag cagcgccgta gccggcgtca gctccgcagc
    13681 aaaccgcaga tcacgcagag gaaagccggg gtctgcaacc gccacgctca gcgtatcggc
    13741 cgtagcctgc cagccgtctt cgcgcagctt cacccgggcc tcaccggcaa tgcctgccgc
    13801 caccacttcg ccgtagtggg attgaacgcc ttcgagggtc acgaccacgt cgcccgacag
    13861 cgccccgtca ccgagggtca ggctgcccgt cactgcgacg tttcccgcgt cgacgtcgta
    13921 aggctgatcc cagccagcaa ggaacgtggc cagcagcggc gctctcagcc gctggcgagc
    13981 cgatagctcg acgaccatgg tcgacgactc ggcgtccgca cgaatctgcc cagaggcgga
    14041 gagctggagg gcggggaccg ttgccgcaaa cgcgccccgc gcgctgccgg ggtccatggc
    14101 gagggaatcg atgcgaaggt cgacgtaggg agcaagtgcg aagttctccc agcgcgcggc
    14161 atccgcagcc agcgcggcgc gaaccgggct ccccagggtg agcgtcattg gttcgatccc
    14221 gacttcgagc gcaaggccgt cgagggtatc cagacggata ccctcgagac tccaagacgc
    14281 acccgaaacg gcggcaccgg tcaccaccat gcccgggtcg atggacagct tcgcgccgga
    14341 tctgtccaac ccggccgaca gggttccacg cgccgcaacg tcatggggca ggtcagcttc
    14401 gagctgccag gcgccggaga cacggacgag ctcggtagcc tcgtcttcag gccaggggcc
    14461 ctccgcttcg gccaccgcga agcctccgcg ccaagccccg ccgtccacgc gcgcctgcag
    14521 cccggatccc aaccgccagc cattctcgct aagctcgatg gggccccggc cttccagggt
    14581 gacggcgagt tcgccatcct caacgggcag ctccagattc caggcaataa ccgcctcgcc
    14641 cgccagacgc cgagcatcgg ccgagacagc cagggagttg gccggtcgct ctagctgctg
    14701 gatggccagg ccaaccgagc ccgcggcatc tgcgcgcatg gaaagccgga gcgcgccaag
    14761 gggctcgacg ccgctcacct cgcctgccag gctggctgcc gctggcgtga atccaaacgt
    14821 accggcaaag acgagctctc ccgtgcgcaa acggacctca tccacctgaa cgctctccac
    14881 cggcagttcc tgccaggcgt ccaggaacag gcccggcagc acaacgatct gctctccaag
    14941 cgccccgggt tccgcatcgc cgctggagtc ctcgacgtac accgtcagca gcgcagcgcc
    15001 cacgaacgcc agccttcgct cccggacgcc cgaccagtta taaccaacgg tcagttgctc
    15061 ggcccgcagc tcattgccct gcatgctcgc gacgagccga tccacggcaa tttcacccca
    15121 gcgcggaaat cgcacgtcca gctcccgcac ctcgatgccg acagcggccg cctcgcgcac
    15181 cgccagcgtc tcggccagcc agggcagcga cgcgtaagcg cctgccagca ggccggccag
    15241 aagaatcacg attgccagga gccacttcac ctacccggcc ccttcgtagc gcccatgacg
    15301 ggaaatctcc ttgtgcagca tccggcgcac ccaaccatag gccaggccgc cgaacaagca
    15361 gttgcagacg gccgccgcgg caaagattcc cgcatagccg aagagcgttt ccatgatcaa
    15421 agccattggt atgtagagaa cgatcattcg caacagcgaa agcaccaggg tgggcatggg
    15481 cttgcccaac gcgatgaagg tcgagctcgc caccatgccc acaccgagaa ggccgtaggt
    15541 agctggaagc aacaacaggt agaggt
//
LOCUS       DBNHLM010000097         2069 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1630395, whole genome shotgun sequence.
ACCESSION   DBNHLM010000097 DBNHLM010000000
VERSION     DBNHLM010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1630395"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="AC16QF_04600"
     CDS             complement(<1..346)
                     /locus_tag="AC16QF_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447864.1"
                     /translation="MRPGGTNPLDSRGLRRCSQAPYPYDAYPLRAELLYLHPPLQPGQ
                     SAFAQLRVQNVRGGESESFALTPGSTKQLSDGSLLAAEGLGPRPAILLRHRRAPGKPL
                     ALVSSILLGLGLL"
     gene            398..1465
                     /locus_tag="AC16QF_04605"
     CDS             398..1465
                     /locus_tag="AC16QF_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013698.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIR6447865.1"
                     /translation="MERYIGMDVHAASCTLAVISEKGRKLRDFPVETNGQALVEAVRM
                     IPGHKHLVMEEGLQSAWLYETLSPHVDELVVAGITQSRGHKSDKRDAYGLAEKLRVGN
                     LDKPIFKSPRQFTRLRELSRIHMTLVGDVVRGQSRIKSLYRSRGIFVSGVNVYGVRHR
                     EQWQAKLSSSAQTRATRLYDHLDFLLEQKKQAEADLLREAKKHPIVGVLETAPGFGPI
                     RAARLVPIVVTPHRFRTKRQFWSYCGLGIVTRSSSDWIQAADGGWIKARVPQTRGLSC
                     QHNHFLKSLFKGAATTVITQVNKDPIHARYERLLDGGTKPTLAKLSLARMIAATVLRM
                     WKDEKEYDSRRGDPLTATREG"
BASE COUNT          443 a          603 c          672 g          351 t
ORIGIN      
        1 ggagcagtcc gagcccgagc aggatgctgg ataccagcgc cagcggtttg cccggcgcgc
       61 gtcgatgccg cagcaggatg gcgggccgcg gcccgagacc ctcggcggcg agcagcgagc
      121 cgtcggacaa ctgcttcgtc gaacccggcg tcagcgcaaa cgattccgac tcgcccccgc
      181 gcacattctg cacgcgcagc tgagcgaagg cgctctggcc gggttgcagc ggcgggtgca
      241 gatagagcag ctcggcgcgc agcggataag catcataagg gtatggcgcc tgcgagcagc
      301 gacgtaatcc cctggaatcg agcgggttgg tgcctcccgg tctcatcgga catcctcgag
      361 ttggcgattc aacgatcccc aacgagagga ggcaccggtg gaacgataca tcgggatgga
      421 cgtacacgcc gcgagctgca cgctggcggt gatttccgag aagggccgga agcttcgaga
      481 ttttccggtc gagaccaatg gccaggcgct tgtcgaggct gttcggatga ttcccgggca
      541 caagcatctg gttatggagg aagggcttca gagcgcttgg ttgtacgaga cactgagtcc
      601 tcatgtggac gaactcgtgg ttgcggggat cacgcagagc cgagggcaca agagcgacaa
      661 gcgcgatgcg tatggattgg ccgagaagct tcgtgtggga aacctcgaca agccgatttt
      721 caaatcaccg cgtcagttca ctcgcctgcg cgagctctca cggattcaca tgacgctggt
      781 tggggacgtt gtgcgtggcc agtctcggat caagagcttg tatcgctcgc gcggcatctt
      841 cgtttccggc gtgaacgtct acggtgtccg tcaccgggag cagtggcagg cgaaactgtc
      901 ctccagtgcg cagacgagag cgacaaggct ttacgatcat ctcgacttct tgttggaaca
      961 gaagaagcag gcagaggccg atctacttcg ggaggcgaag aaacatccca tcgtcggggt
     1021 tctggagacg gcgccgggct tcgggccgat ccgcgccgca cggctggttc cgatcgtcgt
     1081 tacgcctcac cgctttcgca cgaagcggca gttctggagc tactgcgggc tcgggatcgt
     1141 gacgcgatcg agttcggact ggatccaagc ggccgacggc ggctggatca aggcacgcgt
     1201 tccacagacc cgaggactct cgtgccaaca caaccacttc ttgaagagcc tcttcaaagg
     1261 cgcggcgacg acggtgatca cacaggtcaa caaagatccc atccatgcgc gctacgagcg
     1321 acttctcgat ggaggcacga agccgacact cgcgaagctg agcctagcgc gcatgatcgc
     1381 agcaacggtg ttgcggatgt ggaaggacga gaaggagtac gactccaggc gaggagatcc
     1441 attgacagcc acgcgcgaag gctaaacgat ggctcggcca ggggagtcga cgagaaagcg
     1501 gcccttcaag tacgcagccg cgaagagtgt tcacatgcca cgctttgcgt ggccgcggag
     1561 gagagcatcc atgaatttcc tggcctacgg cccacagcct ggaggtccta cggataagct
     1621 atgcccctcc gaagaaccca acgaagcaat ggccgaccga ggcccagatg gaaggatggt
     1681 tcccacgttc atgtggagaa cacacatgcc aataaatcgc gctgcaatga ggtcgcccac
     1741 gaaccgaatc gtcggaccga gccactcgta acggttccgc agctgctcaa gcggagcgac
     1801 gagaacaacg tctccactcg agaccgtcta gccagaacgg gagcagacgc ccggatcaaa
     1861 gaatctgcgg cgatggggga agtgcgtctt gcgctccgac cctatggaag gcaattcaag
     1921 cggggttttc ggcgaggttc gataacgcgg gattctgttg agttgacagt caggcttgcg
     1981 gggcgccgtt ggcctcctct cacgccaacc gagcaactac cctcgcactc aaacccggcc
     2041 gggtgggcga ctccgaacgg ggtcgttcg
//
LOCUS       DBNHLM010000098         7456 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_373773, whole genome shotgun sequence.
ACCESSION   DBNHLM010000098 DBNHLM010000000
VERSION     DBNHLM010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_373773"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1369)
                     /locus_tag="AC16QF_04610"
     CDS             complement(113..1369)
                     /locus_tag="AC16QF_04610"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6447866.1"
                     /translation="MKLKTGADVDATPLEEIDVSRPELYLNDTWQPWFARLRREAPVH
                     FLADSVNGPFWSVSSHQLIKEVDRNHQVFSSEKGGIAIVDPYVMEEGQVQGRSFIALD
                     EPKHMPQRMAVAPTVAPKNLAELEPLIRERAIDILDHLPVGETFNWVQEVSIELTARM
                     LATLFGFPYEDRRKLVYWSDLATAVPEVTGDDSIDLSKRYEELMGAAAAFYQLWAERA
                     QEPPKFDLISMLIHNEQTAKMNEDPEQFLGNILLLIVGGNDTTRNSISGGVIALNRYP
                     GEYQKLRDNPGLIPNMVSEIVRWQTPVIHMRRTALQDCELGGKQIREGDKVVMWYLSG
                     NRDETVFDDPDRLIIDRPNARAHVAFGFGVHRCMGNRLAELQLRILWEEIMKRFHTVE
                     VVGDVERLSNNFIRGIKDVPVRLHPT"
     gene            complement(1474..1911)
                     /locus_tag="AC16QF_04615"
     CDS             complement(1474..1911)
                     /locus_tag="AC16QF_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HIR6447867.1"
                     /translation="MSDEPSYGTGDATFRAAGGEAGIRRLVDAFYDIMERDPRYRRIW
                     DWHPPDNEVSRDKLARFLCAWMGGPRLFSEKYGRISIPSAHAHLGVTAVERDLWLDCM
                     ARALAQQDYPESLVAYLLKELAVPAERIRQVTEGGAKSETGDR"
     gene            complement(1999..3261)
                     /locus_tag="AC16QF_04620"
     CDS             complement(1999..3261)
                     /locus_tag="AC16QF_04620"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6447868.1"
                     /translation="MDWQQYELEHEQRLDNARAHAATLALEEIDVAHWDLMRFDTIWP
                     YFERLRREAPVYHHENSIVGPYWSVTSYELVKAVDTDARRFSSEPTIGLNVPPEQDEA
                     SSFIAMDDPKHAEQRKAVAPVAGPRNLAALAPTIRQRVADILDGLPVGEPFNWVERVS
                     IELTTQMLATLFDFPFEERYKLTRWSDIAISPRKAGLWDTLEERERELDECLSRMTEL
                     RDERIRQPGGHDLLSMLAHSESTRNMPPRQFLGNLLLLIVGGNDTTRNSISGGVLAMN
                     RWPEEFEKLKADRSKIPNAVAEIIRWQTPLAFMRRTTTEDVEIGGQTIPAGERVLMWY
                     ASGNRDENVFENADLFEVDRGNARQHLSFGFGVHRCMGNRVAELQLKILWEELLERYS
                     RIEVAGAPVRAISNFVRGYTHLPVKLHT"
     gene            3498..3986
                     /locus_tag="AC16QF_04625"
     CDS             3498..3986
                     /locus_tag="AC16QF_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447869.1"
                     /translation="MDIATLGAVGEFVGGIAVLITLIYLAYQTRLSARMHQQSVSDLE
                     SQIISQNADGWVQVFLQTAKDERLSAIYEKLVSARPLDPSEFASAQLFLSAYFLRLEN
                     IEFQRRNLNWEAMGIDDLLRKQVGTFSASPDFQSWWSKASSEGFSDWFVDAVNSILEA
                     RS"
     gene            4018..5577
                     /locus_tag="AC16QF_04630"
     CDS             4018..5577
                     /locus_tag="AC16QF_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6447870.1"
                     /translation="MNEQTQGKVAGFRRHRLFRIATIYVVVGWLSIQAADIVLEAFES
                     PAWIMRAFLILVLAGLPLTLIATWLLDRTRATRPSNPIVPLLTIGLALLISLGAYVYF
                     SKEVFEGGGDSDPVLQVRASNPVLAVLPFANMSDEESNQHLADGLTEDIITLLAQSPG
                     VEVIARNSTFKYKGRNPDIRDVGRDLGADYVIEGSIRPMGGRVRVTVQVIDAGSGAHM
                     WADKYDRPLGDFFEIQDEVTLGVAAAVGDAVFKEEFSAIAHSRTDDLTAWALVSQSEM
                     AFSHEITGAENGVEYARQAVKLDPEYALAHAVLGRALALNAMYYNDPEIAAEAVAEAR
                     LAAQLDPDDPKVNAYLAFTLLTTGQAADALSYAERVPKISPSYAEGLAYYADILTHNG
                     RSRESLPYFDKAMRLAPNAPQLPWFYWLHGEALIHHGNFTEAEASLLTATRLYQTVHP
                     ILLYLLAGAQLQLGKAEEARASLERGAAAEAQPLGESIARFQTLFPDGGGEYYEMMWT
                     DLTALSAAGPT"
     gene            5599..6012
                     /locus_tag="AC16QF_04635"
     CDS             5599..6012
                     /locus_tag="AC16QF_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447871.1"
                     /translation="MSVQVRQYQRSSIAEGRSKGHEIWRNTIVQNTDAAALAKANSGE
                     SLSERERVQLGFFADELFISSAASDQFSRHSGTFYDDEAYWRYLADILQRNPGLLPEW
                     DRIQKSVMDLNFPEYRAQVNAALADLRPGVAKADS"
     gene            complement(6179..7309)
                     /locus_tag="AC16QF_04640"
     CDS             complement(6179..7309)
                     /locus_tag="AC16QF_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005230 - extracellular ligand-gated
                     monoatomic ion channel activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligand-gated ion channel"
                     /protein_id="HIR6447872.1"
                     /translation="MSPTQRISTLLTVLLLLLLVGWRGLAAVYQASGAVTYEHNPGEL
                     TESLSPQIPIAEATAGQHVIFMGMHIERIYELSLQSRTFTADGYVWIEWSRRTQQFME
                     AQGLRASELIHLVNRIERWDSSFEPETEEPAILSAGRRYQRFHFSSRFHDDQINFERD
                     PFDVLILPIMVEIAPDVLSDKYAQALLYPHQQQDGLLGESGSLSGYRLQGASLQALLH
                     RYPSRFGSWYQPTKSLVRLEIQYRADYWSAFVRWVLPILVVMSIVILTPTVVGSHSDI
                     RLAVPTTALLALVFMHQAYRSGLPPLPYLTLLDAMFSWGYLTCVGLFALFTWGTNVYA
                     SAPEHKKLAAATQIDRVDVVFQVLSLGTFVVLILVLWLSTGS"
BASE COUNT         1493 a         2296 c         2226 g         1441 t
ORIGIN      
        1 tgctgtggcg atgatctgcg tggatgcgat cgccaaggtt tccgcgctca cgacggaggg
       61 tgccttaggg ctggaggaag gcaacgtcga gagcgccgcc tgaacgacga gctcaagttg
      121 ggtggagcct tacggggacg tccttgattc ctctgatgaa gttgttggac aaacgctcga
      181 cgtcgcccac cacttcaacg gtgtgaaagc gcttcatgat ctcttcccag agaatgcgca
      241 gctgaagctc cgccagccgg ttacccatgc agcggtgcac cccgaagccg aacgcgacgt
      301 gggccctggc gttcgggcga tcgatgatca gccggtccgg atcatcgaaa acagtctcgt
      361 ctcgattacc ggagaggtac cacatcacca ccttgtcgcc ctccctgatt tgcttgccac
      421 cgagctcgca atcctgcagg gccgttcggc gcatgtgaat gaccggtgtc tgccaacgaa
      481 ctatttcaga aaccatgttt gggatcaggc cgggattgtc ccgcagtttc tgatactcac
      541 ccggatagcg gttcagagcg atcacgcctc cgctgatgct gttccgggtg gtgtcgtttc
      601 cgcccacgat caacagcagt atgttgccga gaaactgctc agggtcctcg ttcatcttcg
      661 cggtctgctc gttgtggatc agcatggaga tcagatcgaa ttttggcggt tcctgggccc
      721 gttccgccca caactgatag aacgccgccg ccgcgcccat cagctcctcg tagcgcttgc
      781 tcagatcgat gctgtcgtct ccggtcacct cgggcaccgc ggtagccagg tccgaccagt
      841 acactagctt gcggcggtcc tcgtacggga aaccaaacag cgtggccagc atccgggccg
      901 tcagctcgat ggatacttcc tgcacccagt tgaaggtttc gccaaccggc aggtggtcga
      961 ggatgtcgat ggctctctca cgaatcagcg gctccagctc cgccaggttc ttcggagcga
     1021 ccgtgggagc gacggccatt ctctgcggca tgtgtttcgg ctcgtccagc gcgatgaagc
     1081 tgcggccctg tacctggcct tcctccatca cgtaagggtc gacgattgcg atcccgcctt
     1141 tctccgacga gaacacctgg tggtttctgt cgacctcctt gatcagttga tgactggaga
     1201 ccgaccagaa agggccgttc acgctgtccg caagaaagtg cacgggcgct tcccttctga
     1261 ggcgggcgaa ccacggctgc caggtgtcgt tcaggtaaag ttccggacga ctgacgtcga
     1321 tctcctcgag gggggtggca tcgacatccg cacctgtctt caacttcatc cgagcctcct
     1381 gatcaagccg taaagtgaac gatttcagaa gcatatcacc gaccggaaat ccgatcgcga
     1441 ccgggcgcgc ttcgaggcta ctgctgagag ctgctaacgg tctccagttt cagattttgc
     1501 tccgccctcg gttacctgtc gaatacgctc ggcgggtacg gcgagctcct tgaggaggta
     1561 tgcgaccagc gactcggggt aatcctgctg cgcaagcgct cgggccatgc agtcgagcca
     1621 gagatcgcgc tcgacggcgg tgacgccaag gtgcgcgtgg gcggacggaa tgctgatgcg
     1681 accgtacttc tcgctgaaca accgcggacc gcccatccag gcgcagagaa agcgcgcgag
     1741 cttgtcgcgt gatacttcgt tgtccggcgg gtgccagtcc cagatacgcc ggtatctggg
     1801 gtcccgctcc atgatgtcgt aaaaagcgtc taccaaccgt cggatgccgg cttccccacc
     1861 ggcggctctg aaggtggcat ctcccgtgcc gtagctgggc tcgtcgctca cgaccgctgc
     1921 gccccaggag agacgtgttc gccgccggta acgtcgtgaa aggtcacctc gagaggccca
     1981 ttgcccgatg acccacggtc aggtgtgcaa cttcacgggc aggtgagtgt agccgcgaac
     2041 gaagttggag atggccctca ccggagcgcc tgccacttca atacggctgt aacgctccag
     2101 cagctcctcc cagaggatct tgagctgcag ctcggcgacg cgattgccca tgcagcggtg
     2161 cacaccgaag ccgaaggaga ggtgctggcg agcgttcccg cgatcgacct caaagaggtc
     2221 ggcattctcg aaaacgttct cgtcccgatt cccggacgcg taccacatca ataccctttc
     2281 tcccgcggga atggtctggc caccaatctc aacatcctcc gtcgtcgtgc gacgcatgaa
     2341 cgccagcggc gtctgccagc ggatgatctc ggccacggcg ttcggaatct tcgagcggtc
     2401 ggctttgagc ttctcgaact cttccggcca ccgattcatg gcaagcacac cgcccgagat
     2461 ggaatttctc gtggtgtcgt tgccaccaac gatgagcaga agcaggttgc ccaggaactg
     2521 gcgcggcggc atgttgcggg tactttcgct gtgcgcaagc atcgagagca ggtcgtgacc
     2581 gcccggttgg cggatccgct cgtcgcgcag ctcggtcatg cgcgacaggc actcgtcgag
     2641 ctcccgctcg cgctcttcca gcgtgtccca gaggccggct ttgcgtggag aaatcgcgat
     2701 gtccgaccag cgcgtcagct tgtagcgttc ctcgaaaggg aaatcgaaca gggtcgcgag
     2761 catctgcgtg gtgagctcga tggatacgcg ctccacccag ttgaagggct cacccacagg
     2821 aagaccgtcg agaatgtcag ccacgcgttg cctgatcgtc ggcgccagcg cagcgaggtt
     2881 acggggacct gccacaggcg cgaccgcctt gcgctgctcc gcgtgcttcg ggtcgtccat
     2941 ggcaatgaag ctcgacgctt cgtcctgctc cggcggcacg ttcagcccga tggtcggctc
     3001 ggaagaaaaa cgacgcgcgt cggtatcgac ggccttgacc agctcgtagc tcgtcacgga
     3061 ccagtaggga cccacgatgc tgttttcatg atgatagacg ggcgcttcgc ggcgcaggcg
     3121 ctcgaagtac ggccagatcg tgtcgaatcg catgaggtcc cagtgagcaa cgtcaatctc
     3181 ttcgagggcc agcgttgccg cgtgggcacg cgcattgtcc agtcgctgct cgtgctccag
     3241 ctcgtattgc tgccagtcca tcgtcgaccc caatcaagtc cagcgcagat catactccga
     3301 agcgcccgtg ctgctcgaca cgccctgcca agcccccgga gagctccgcg gagggcccga
     3361 gccggtcatg aagcgagcat ctccgcccga tgcgccttct ctacaagact cttttctcgt
     3421 agattggcaa ggagcgggag tggagaagaa cgatgcaggt gagtcagacc gcgtcgcttt
     3481 acgggaagtc ctagatcatg gatatcgcca cgttgggtgc cgtcggggag ttcgtcggcg
     3541 gaatagccgt tctgatcacg ctgatttacc tcgcttatca aacccggctc agcgcgcgga
     3601 tgcatcaaca gagcgtgagt gatctggagt ctcagatcat aagccagaac gccgatggtt
     3661 gggttcaggt gttcctgcaa acagccaagg acgagcgttt gtcggccatc tacgaaaagc
     3721 tcgtgagcgc acgacctctg gatccatcgg aatttgccag cgcacagctt ttcctgtcgg
     3781 catacttcct gcgactggaa aacatcgaat ttcagcgtcg gaatctaaac tgggaagcga
     3841 tgggaataga tgacctgttg agaaaacagg tcggcacgtt ctccgcctca ccggactttc
     3901 agagctggtg gtcgaaggcg tcgtcggaag gattctccga ttggtttgtc gacgccgtca
     3961 attcgatcct tgaggcccgc agctgacgcc agcctcagtg ctaaaatcat ggagcacatg
     4021 aacgaacaaa cccagggaaa ggtcgccggg ttccggcgcc atcgtctgtt ccggatcgcc
     4081 accatctacg tggtggttgg ctggctgtcc attcaggccg ctgacatcgt cctcgaggcg
     4141 ttcgagtccc ccgcgtggat catgagagcg tttttgatcc tggtgctcgc gggccttccg
     4201 ctgacactga tcgcaacctg gctgctggac cggacgcggg cgacccggcc gtcgaatccc
     4261 atcgtgcctc tcctgaccat tggccttgct ctcctgatct cgctgggtgc ttacgtgtac
     4321 ttctccaaag aggtatttga aggtggcggc gactccgatc cggtgctgca ggtccgggct
     4381 tccaaccctg ttctggccgt gctgccattc gcgaacatgt ccgatgaaga gagcaaccag
     4441 catctcgccg atggcctgac cgaggacatc attaccctcc tcgcgcagtc gccgggcgtc
     4501 gaagtgatcg cgcgaaactc cactttcaag tacaagggcc ggaatccgga tattagagac
     4561 gtcggccgcg acctcggagc ggactacgtg atcgagggca gcatccgtcc gatgggcgga
     4621 cgcgtccgcg taaccgtaca ggtcattgac gccggctccg gcgcacacat gtgggccgac
     4681 aagtacgaca ggcccttggg cgatttcttc gagatccagg acgaggtcac tctgggcgtc
     4741 gcagcagcgg tgggcgatgc cgtgttcaaa gaggagttct ccgcaattgc ccactcccgc
     4801 accgacgacc tcacggcctg ggcgttggtc tcgcaatccg aaatggcctt cagccacgag
     4861 atcaccggtg ccgagaacgg cgtggagtat gctcggcagg cggtgaagct cgacccggag
     4921 tatgccctgg cccatgcggt tctagggcga gccctggccc tgaacgccat gtattacaac
     4981 gaccccgaga ttgccgcaga agccgtggct gaggctcgtc tggccgcaca gctcgacccg
     5041 gacgacccca aggtcaacgc ctaccttgcc ttcactctgc tgacgacggg ccaggcggcg
     5101 gacgcgctct cctatgccga gcgggttccg aagatcagcc cgagctatgc cgaggggctg
     5161 gcctactatg cggacatcct cacgcacaac ggacgttcgc gagaatcgct cccctacttc
     5221 gataaggcca tgcggctcgc gccgaacgcc ccccagctac cctggttcta ctggttgcat
     5281 ggggaagcgt tgattcatca cggaaacttc acggaagcgg aagcatccct gctgaccgcc
     5341 acccgcttgt accagaccgt gcacccaatc cttctctatc tgctcgccgg cgcgcagctg
     5401 cagctcggca aagccgagga ggcacgggca agcctcgagc gtggggcagc ggctgaggcc
     5461 cagccgctag gtgagagcat cgcaaggttt cagaccctgt ttcccgacgg cggcggcgag
     5521 tactacgaga tgatgtggac cgacctgact gctctttcag cggcaggtcc aacctagcga
     5581 aggcaacggt tgggattgat gagtgttcaa gtacgtcaat accaaagaag ctccatagcc
     5641 gaaggccgca gcaagggaca cgagatttgg agaaacacga tcgtccagaa taccgatgct
     5701 gctgcgctgg ccaaggcaaa cagcggagag agtctctcgg aacgagaacg agtccagctg
     5761 ggattttttg cagacgaact attcatctct tcagccgcat ctgatcaatt cagccgccac
     5821 tcaggcactt tctatgacga cgaggcttac tggaggtatc tggcagatat ccttcagagg
     5881 aatccaggac tcctgcctga gtgggacaga atccagaaga gcgttatgga tctgaatttc
     5941 ccggagtatc gggcgcaggt caatgcggct ctcgcagacc tcagacccgg cgtagcaaag
     6001 gccgattcct gatttgacga acgttcacga gacatcagga ccggcctcgc tcattcgcac
     6061 cggacccagt caagatccct gatcagccag gacggaacta cgaagaccgc gcgtgcccgg
     6121 agcctaccac ctccgttcga gcgatccggc cggtatggtc cctattcact tgatcccatc
     6181 acgagccggt gctcaaccac agcaccagta ttaggaccac gaaagtgcct aacgacagca
     6241 cctggaagac cacgtcgacg cgatcgatct gagtcgcagc ggccagtttt ttgtgctcag
     6301 gagcactggc gtacacgttg gtgccccacg tgaacagagc gaacagccca acacaagtca
     6361 ggtatcccca gctgaacatg gcgtccaaca gcgtcaggta cggaagcggg ggaaggccgg
     6421 accgatacgc ctggtgcatg aagaccaatg ccagcagggc ggtcgtcggc acggccagcc
     6481 ggatatcaga gtgggacccg acgaccgtcg gcgtcaagat tacgatggac atgaccacca
     6541 ggataggcag gacccagcgc acaaaggcgc tccagtagtc agcccggtat tggatttcca
     6601 gccggaccaa gcttttcgtg ggttgatacc aagacccaaa gcgcgacgga tagcggtgca
     6661 gcagcgcctg cagtgaagca ccctgtagcc ggtagccact caggctgccc gactcaccga
     6721 gcaacccgtc ctgctgttgg tgggggtaga gcagggcctg ggcgtacttg tcggacagaa
     6781 catctggcgc gatctcgacc atgatgggaa ggatcaggac atcgaaggga tcgcgctcaa
     6841 agtttatctg atcgtcgtgg aaacgacttg agaagtggaa tcgctgatac cggcgccccg
     6901 ccgagagtat cgcgggctcc tccgtttccg gctcgaaact cgagtcccac cgttcaatcc
     6961 ggttgaccag atgaatgagc tccgacgccc gcagtccctg ggcctccatg aactgttgcg
     7021 tacgcctcga ccattcgatc cagacatagc catccgccgt gaacgtccgc gactgcaggg
     7081 agagttcgta gatcctctct atgtgcatgc ccataaaaat cacgtgctga cccgccgtcg
     7141 cctcagcgat cggaatctgg gggctcaacg actcggtgag ttcgcccggg ttatgttcat
     7201 acgtcacggc ccccgatgcc tggtacacag ccgcgagccc tcgccaacca accagcaaga
     7261 gaagcagcag caccgtcagg agagtgctga tccgctgcgt cgggctcatg ggtcgtctcc
     7321 ggattcgccc gagcggaacc ggctcgcatc gggccgctcc tggctgaagt cacacacaaa
     7381 cccctcgttc tcgggaagcc acggcgacca cgctgtgggg ataatcggtt cttcggcatg
     7441 caggatcttg aactgg
//
LOCUS       DBNHLM010000099         2702 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_433569, whole genome shotgun sequence.
ACCESSION   DBNHLM010000099 DBNHLM010000000
VERSION     DBNHLM010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_433569"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..991)
                     /locus_tag="AC16QF_04645"
     CDS             complement(200..991)
                     /locus_tag="AC16QF_04645"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR6447873.1"
                     /translation="MRENALLAAWREGRQTNGCWLSMGNAYSAEALGQLGFDYVCIDL
                     QHGLVDYQDLTYMLPAVSRGTSTPLVRVPWNEPYEIMKALDAGAYGVIVPMVNNAEDA
                     ARAVDACRYPPLGSRSFGPIRGALYGGRGYAAQANEQMACIVMVETREGLDKLEEIVT
                     TPGVDGVYIGPADLALALGLPALGDTDVPEHVAEVTRILEVCHRHELPAGIHTSSLDY
                     CQKRLAAGFDFVTLGSDAGFMTRTAAQELAAARGTAEQEREKTGY"
     gene            1109..2161
                     /locus_tag="AC16QF_04650"
     CDS             1109..2161
                     /locus_tag="AC16QF_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0003714 - transcription corepressor
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_function: GO:0140297 - DNA-binding transcription factor
                     binding [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HIR6447874.1"
                     /translation="MDRKKVVVQLPAISKAKAVEDRYALELEALAPLADIVEVDAQTP
                     AEFGEGASDADAIITSWGVKIDREVIGRLERCVVIGVGSVGVDMVDVDAATKAGIVVT
                     NVPDVFIEEVADHAMMLLLAAVRRTRLMGDLVRSGDWLQARPMLSKVPRLMGRTLGLF
                     SFGNVARCTARRARAFGLHVIAFDPYVSELAISDAGVEPVSFAELLERSDYLSVHAPL
                     NDETRHVLNGDAFARMKPGAVIVNTARGPIIKEDDLIKALENGQIGAAGLDVLEREPP
                     DPANPLLDMANVIITPHVASASTRMRPETRRRVGREVALVLRGKWPMSCVNPTVLPRA
                     SLERWQPYPTQRGPNR"
     gene            2195..>2702
                     /locus_tag="AC16QF_04655"
     CDS             2195..>2702
                     /locus_tag="AC16QF_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447875.1"
                     /translation="MWQPSFSKDLPEVAQRSFRNEQLTELTLPVAFTLLEGGIVGVIA
                     AKIFHVPPIVLAVITAAPMFGNISSFIWNRLATAKLKVRFANVVQILTMACVLAIALA
                     PVNQIGAVLLVAGMIMSRLLITGIITVRSVIWSLNYNRALRARTAGRLQIITSLVTVL
                     ISSLVGPLL"
BASE COUNT          450 a          917 c          860 g          475 t
ORIGIN      
        1 ctcccggctc atctggtagg tccacgcgtt cagtcgctcc ggccggttca gcgtgagaac
       61 ggcgatggaa tcctgcgcct cgaataggat ctgctcgtag ccggtcacct tggtctcctc
      121 tgcctcattc gggcctaagt atgaggcaat cgggccgtga agccacccgg ggcgatcggc
      181 tggtctggcg agacaggact cagtagcccg tcttctcccg ctcctgctcc gcggtgcccc
      241 gcgcggccgc aagctcctgg gcggcggtgc gcgtcatgaa accggcgtcg ctgcccaggg
      301 tgacgaagtc gaacccggca gccaggcgtt tctggcagta gtccaggctt gaggtgtgaa
      361 tgcccgccgg cagctcatgg cgatgacaga cctccagaat gcgggtgact tccgccacgt
      421 gctcgggcac gtccgtgtcg ccaagcgccg gaaggcccag cgcaagcgcc agatcggccg
      481 gtccgatgta gaccccgtcc acgcccggcg tggtcacgat ctcctcgagc ttgtccaggc
      541 cctcgcgggt ctccaccatc acgatgcagg ccatctgctc gttggcctgg gccgcatagc
      601 cacgcccgcc gtaaagcgcc ccgcgaatgg gcccgaaaga gcggctgccc agcggcgggt
      661 agcgacaggc gtcgacggcc cgcgccgcgt cttcggcgtt gttgaccatg ggcacgatga
      721 cgccgtaagc gccggcgtcc agcgccttca tgatctcgta gggctcgttc cagggtacgc
      781 gcaccagcgg cgtcgatgtg cctcgggaca cggcgggcag catgtaggtc agatcctggt
      841 agtcgaccag gccgtgctgc agatcgatgc acacgtagtc gaagccgagc tgtcccagcg
      901 cctctgcgga gtacgcgttt cccatggaga gccagcagcc attggtctgc cggccttccc
      961 gccacgctgc cagcaatgcg ttttcgcgca cgttcttccc tccccggatc tacccccaaa
     1021 ccttatcaca ggtaaggaat gctgctgaca ctcgaaacgc cggcgtgcga aaatcggcgt
     1081 tctcatttgc acccggcgga gctcgacgat ggacaggaaa aaggtggtcg ttcagcttcc
     1141 cgccatctcg aaggcaaagg cggttgaaga ccgctacgcg ctcgagctcg aggccctggc
     1201 acctctggcg gacatcgtcg aggtggacgc gcaaacgccc gccgagttcg gcgaaggagc
     1261 gagcgatgcg gatgccatca tcacctcgtg gggcgtgaag atcgaccggg aggtgatcgg
     1321 ccgtctcgag cgctgcgtgg tgatcggggt ggggagcgtg ggcgtcgaca tggtcgacgt
     1381 ggacgctgcc acgaaggccg gcatcgtggt gaccaacgtt cccgacgtgt tcatcgagga
     1441 ggtggccgac catgccatga tgctccttct cgccgcggtg cggcgcacgc gcctgatggg
     1501 cgacctggta cgaagcggcg actggcttca ggcacgcccg atgctgagca aggtgcctcg
     1561 gctcatgggc cggacgctgg gcctgttctc tttcggcaac gttgcccgtt gcacggcgcg
     1621 aagagcccgg gcgttcggcc tccacgtcat cgccttcgac ccgtacgtga gcgagctggc
     1681 aatcagcgac gcgggcgtgg agcccgtctc tttcgccgag ctgctggaac gctccgacta
     1741 cctgtccgtt cacgcgccgc tgaacgacga gacgcgccac gttctcaacg gcgatgcgtt
     1801 tgctcgaatg aagcccggcg cggtgatcgt gaacaccgcc cgaggcccca tcataaagga
     1861 agacgatctg atcaaagccc tcgagaacgg gcagatcgga gccgccggtc tggacgtgct
     1921 cgaacgcgag ccgccagatc ccgccaaccc gcttctggac atggccaacg tcatcatcac
     1981 tcctcacgtc gcctccgctt ctacccggat gcgtccggag acccgccgcc gtgtgggcag
     2041 ggaggtggcc ctggtgctgc gaggaaagtg gcccatgagc tgcgtcaacc ccacggtgct
     2101 gccccgggcg tctctcgagc gatggcaacc ctaccccacc cagcggggcc ccaatcggtg
     2161 aggcggcgat caacccggac ggctggctga ccccgtgtgg cagccgtctt tctcgaagga
     2221 cctgccggag gtcgctcagc gcagcttccg caatgagcag ctcaccgagc tgaccctgcc
     2281 cgtcgccttt accctgctcg aaggcggcat cgtcggcgtc atcgcggcca agatctttca
     2341 cgtcccgccc atcgtcctgg ccgtcatcac ggccgccccg atgttcggca acatctccag
     2401 cttcatctgg aacaggctgg ccaccgcgaa gctcaaggta cggttcgcca acgtcgttca
     2461 gatactgacc atggcctgcg tgttggccat tgcccttgct cccgtcaacc agattggcgc
     2521 cgttctgctg gtggcgggga tgatcatgag caggctcctc atcacgggaa tcatcacggt
     2581 ccgcagcgtc atctggagcc tcaactacaa tcgcgcgttg cgggcgcgca ccgccgggcg
     2641 cctgcagatc atcaccagcc tcgtcacggt cctgatcagc agcctcgtgg gccccctgct
     2701 gg
//
LOCUS       DBNHLM010000100         7327 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_14709, whole genome shotgun sequence.
ACCESSION   DBNHLM010000100 DBNHLM010000000
VERSION     DBNHLM010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_14709"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1223)
                     /locus_tag="AC16QF_04660"
     CDS             complement(90..1223)
                     /locus_tag="AC16QF_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011332951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR6447876.1"
                     /translation="MSKPVVISGAGLWKPDHVITNAELVAAYNAYADRFNAENDAAIG
                     AGDVAPMEHSSEEFIEKASGIKQRYAYVKDGILEVGRMRPRIPHREEDELSHQAEIAL
                     HAARGAMAAAGKAASDIDMVIVSCAYTERSYPAIAIEVQHALGIDGFAFDMLVACSAA
                     TFALQRAHDAIAAGSARCVLVVNPELTSPQVNYRDRDGHFIFGDVSTAVVLESAETCT
                     AAHAYEILGSKALTSYSNNIRSNFGYLAYAEDVDPFCAANLFHQEGRRVFKEVCPMAA
                     EHLKSQVEAVGLDVADVKRWWLHQANLNMNLLISKRLLGRDASAKEAPIVLDEYANTA
                     SAGSIIAFCLHHEDLAAGDHGVICSFGAGYSIGSLVLRKRAAA"
     gene            complement(1224..2174)
                     /gene="folE2"
                     /locus_tag="AC16QF_04665"
     CDS             complement(1224..2174)
                     /gene="folE2"
                     /locus_tag="AC16QF_04665"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HIR6447877.1"
                     /translation="MNKGELRSEKELPDVASHELHQPHVTLDWVGMADIHQPLLVRDG
                     NQTRHVQSRVQIYVDLGDELAKGIHMSRLYLILDEHAETRPLSAAGLKLLLRSVLESH
                     RELSSRAYVQFDFDYYLRRQALVSDNSGWNSYPVSIRGTLIDGEITLEVALNVQYSST
                     CPCSAALARQLIQDQFDEDFGTSGSVAVGDVRAWLGTEQGIVATPHSQRSDARIRVKL
                     REGLIDFPITDLIDEVEATLKTPVQTAVKREDEQEFARLNGQNLMFIEDAGRKLKDQL
                     DADDRLADFWVRVEHHESLHAHDAVGVFTKGVGDGYQAVP"
     gene            complement(2310..2501)
                     /locus_tag="AC16QF_04670"
     CDS             complement(2310..2501)
                     /locus_tag="AC16QF_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008375064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HIR6447878.1"
                     /translation="MDSKLLELLVCPVTKASLTYNKDKDELWCRASGLAYPIRDGIPV
                     MLEEEARELSAEERESLRE"
     gene            complement(2529..3824)
                     /locus_tag="AC16QF_04675"
     CDS             complement(2529..3824)
                     /locus_tag="AC16QF_04675"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR6447879.1"
                     /translation="MGRNVVVMGTQWGDEGKGKIVDLLTDQVAAVVRFQGGHNAGHTL
                     VIEGEKTVLHLIPSGILRREVQCFIGNGVVLEPNALLSEVSGLEARGVPVRERLRISP
                     ACPLILPYHVKLDLAREEARGNQKIGTTGRGIGPAYEDKAARRGVRLGDLFYWQEFAA
                     KLAEVMEYHNFMLERYYGSETVDYAQTLDECAAVAEQIRPMVADIIPLLHEIRESGAN
                     LLFEGAQGALLDIDLGTYPFVTSSNTTAGGTAVGSGFGPRYLDYILGITKAYSTRVGS
                     GPFPTELFDEVGERLATRGNEFGSTTGRPRRCGWFDAVALRQAVQINSISGLCLTKLD
                     VLDGLESICICVGYEGPDGTSGTPRFASEYYSEIRPVYEEVDGWSESTLGIRRLDDLP
                     ANARAYLARIEETVGAPIDIISTGPDRTETIVLKDPFTA"
     gene            complement(3826..5040)
                     /locus_tag="AC16QF_04680"
     CDS             complement(3826..5040)
                     /locus_tag="AC16QF_04680"
                     /inference="COORDINATES: protein motif:HMM:NF009086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR6447880.1"
                     /translation="MVNRKWRLPDGVDELLPPEAWALEQVRRQVLDVFNSWGFDYVEP
                     PVVEYLDALLVGAGPDGGLGHQDDLDLQTLKVVDQRSGRLMGVRADMTAQAVRIDAHS
                     RPSEGVQRLCYAGPVVLANPAAALDSRVPLKAGAEVFGAPGLAADVEVICLLLEILER
                     TGVEAPVLVLGHMGIYLSLVAPLGLDAFTERQLFAAVQSKSEADIGELIGERSDGALL
                     ERLPTLMGGREILREARAALAGGPDGAVAAVDALEELAEAVLVRNPAVEVRYDLAELA
                     GYGYHNGPVFSAYQADQGRAIARGGRYDGIGAPFGRSRPATGFDVNLRQLIGLAGAGT
                     VGQAVWAPWTGSLGGDVAAALAAEVRRLRAAGERVVVALDDEDGAPSGCDRQLSYVSG
                     VWKVERLGRNEG"
     gene            complement(5092..5958)
                     /gene="hflC"
                     /locus_tag="AC16QF_04685"
     CDS             complement(5092..5958)
                     /gene="hflC"
                     /locus_tag="AC16QF_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HIR6447881.1"
                     /translation="MNLKNLIIVVIAIIAVLLLMNSVYTVQETERAILLRFGAVSQAD
                     VPPGLHFKMPIAEEVKKADGRVLTLDSVPETYYTLEKKPLIVDSFAKWRIVDVQRYYT
                     GTSFDERRGQRVLQERVNEGLRNQISRRDMHEVVSGERDQLMQELLGDLNAVMSGAVG
                     IEVIDVRVKRIDLPPDVSSSVYDRMNSERQIEARQYRAQGRELALGIRADAERQTVII
                     EAEAYRSSEQIRGDGDARSANIYASAYNRDPEFYQFYRSINAYAKVFKDKSDILVLDP
                     TSEFFKYLANDS"
     gene            complement(5967..7103)
                     /gene="hflK"
                     /locus_tag="AC16QF_04690"
     CDS             complement(5967..7103)
                     /gene="hflK"
                     /locus_tag="AC16QF_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003380712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HIR6447882.1"
                     /translation="MAWNEPGGGDKDPWGSRGDQGPPDLDEAFKKLQAQLASIFGGGR
                     GGSGGDGSGLNAGMLGIVLVVAAVAYGLFGLYQLDEQERGVVFRFGAAKEDIVLPGLH
                     WNPPIIDVVRKVNVTRVNSKNHQALMLTEDENIVDVSLTVQYVVNDPLKFLVMVRDPE
                     NSLDHAVESALRHVVGSSLMDDVITAGRAAIAAEVQDRLQTYLDTYGTGILVSKVNID
                     ESAPPNQVRDAFNDVQKAKEDEQRVINEANAYAESIIPEARGEAQKQIEQANAYRDQV
                     IARAEGEAERFEKLLTEYSLAKEVTRDRLYIDAMESVLSRSSKIMIDVEGGNNLLYLP
                     LDRLGQTGSPVAGLATDESVRSIADAILREVNERIASGRIRESR"
     gene            complement(7125..>7327)
                     /locus_tag="AC16QF_04695"
     CDS             complement(7125..>7327)
                     /locus_tag="AC16QF_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447883.1"
                     /translation="VLPADDGKTRAWLYRMGAVLGEEITEDGRVALRVRADADLLERL
                     GRTPRVLLQVNDAVPRILPLPN"
BASE COUNT         1264 a         2498 c         2219 g         1346 t
ORIGIN      
        1 gaggttcaag tcctctcctc ggcaccacat tttagagcgt gcccggcgac cccgaaacag
       61 gaattctctt tgagcggcgc gccctcacat cacgccgccg cccgctttct cagcaccagg
      121 ctgccgatcg agtaacccgc accaaacgag cagatcacgc catggtctcc ggcggcgagg
      181 tcctcgtgat gcagacagaa cgcgatgatc gagcccgccg acgccgtgtt ggcatactcg
      241 tccagcacga tgggcgcttc cttcgccgag gcatcgcggc caagcagccg cttgctgatc
      301 agcaggttca tgttcagatt ggcctgatgc aaccaccagc gtttcacatc ggccacgtcc
      361 agtcccaccg cctccacctg ggatttcagg tgctcggccg ccatggggca gacctccttg
      421 aacacacgac ggccttcctg gtgaaagagg ttggcggcgc agaaggggtc cacgtcctcg
      481 gcatatgcca ggtagccgaa attggaccgg atgttgttcg agtagctggt cagcgctttg
      541 ctgccgagaa tttcgtacgc gtgcgcggcg gtgcaggttt ctgcggactc gagcacaacc
      601 gccgtgctca cgtcaccgaa gatgaagtgg ccgtccctgt cgcgatagtt cacctgaggc
      661 gaggtgagtt ccgggttcac caccaatacg catcgcgcgg acccggccgc tatggcgtca
      721 tgagcacgct gcagcgcaaa cgtcgccgct gagcacgcga cgagcatatc gaaggcaaag
      781 ccgtcaatcc ccagggcgtg ctgaacctcg atggcgatag cagggtagga gcgctcagtg
      841 taggcgcagg agacgatgac catgtcgatg tccgacgccg ccttacctgc cgcagccatg
      901 gcgccccggg cggcgtggag agcgatttcg gcctggtggg aaagctcgtc ctcctcgcgg
      961 tgaggaatgc gcggacgcat tcgccccacc tccaggatcc cgtccttcac gtaggcgtaa
     1021 cgctgcttga taccagaggc cttctcgatg aactcctcgc tcgagtgctc catcggcgcg
     1081 acgtcgccag ccccgatggc agcgtcgttc tccgcattga atctgtccgc gtacgcgttg
     1141 taggcggcga cgagttctgc attggtgatg acgtggtcgg gcttccacag cccggcgcca
     1201 ctgatgacga cgggtttcga cacctaggga actgcctggt atccgtcccc gacacccttc
     1261 gtgaacaccc ccacggcgtc gtgggcatgg aggctctcgt ggtgctccac gcgcacccag
     1321 aagtccgcga gacggtcgtc cgcatccagc tgatccttga gcttgcgtcc ggcatcctcg
     1381 atgaacatga gattctggcc gttgagcctg gcgaactcct gctcgtcctc ccgcttgacg
     1441 gcggtctgca ccggcgtctt cagcgtggct tccacctcgt cgatcagatc ggttatggga
     1501 aagtcgatga ggccttcgcg cagcttgacg cgaattcgcg cgtcgctgcg ctggctgtgc
     1561 ggcgtcgcca cgatgccctg ctcggtgccc agccaggcac gaacatcgcc cacggccacg
     1621 ctcccggagg tgccgaagtc ctcgtcgaac tgatcctgaa tcagctgacg cgccagggcc
     1681 gcggagcacg ggcacgtcga cgagtactgc acgttgaggg cgacctccag ggtaatctct
     1741 ccgtcgatca gcgtgcccct gatggacacc ggatacgaat tccagccgga gttgtcgctc
     1801 accagagcct gccggcgcag gtagtaatcg aaatcgaact gcacgtatgc ccgggagctc
     1861 agctcgcgat gggactcgag cacgctgcgc agcagcagct tcaagcccgc cgcgctcaag
     1921 ggccgggtct cggcatgctc gtcgaggatc aggtagaggc gcgacatgtg aatgcccttg
     1981 gccagctcgt cacccaggtc cacgtagatc tgaacacggg actggacgtg cctggtctgg
     2041 tttccatcac gcacgagaag aggctgatgg atgtcggcca tgccgaccca atccagggtc
     2101 acgtgcggct gatgcagctc gtgactggcc acatcgggca gttccttttc gctgcgaagc
     2161 tcgcccttgt tcatgggtct acctgcatgt gttcttcctc cagctacccc ccccgtgaaa
     2221 cgggcggccc cggattgtac ccaatcggcc tgcaacggca cagaaggtgc cgccggccgg
     2281 cgcggagccc ggttccgggg gccggagcat cactccctca ggctttcccg ctcttcggcg
     2341 ctgagctcgc gggcttcctc ctcgagcatc acggggatgc cgtctcggat ggggtaggcg
     2401 aggccgctgg cgcggcacca tagctcgtcc ttgtctttat tgtaggtcag cgacgccttg
     2461 gtgacggggc agaccagcag ttcgagcagc ttcgagtcaa tcgacatgac gttgcgcctc
     2521 caccgatctc aggcagtgaa cggatccttc agaacaatgg tttcggtccg gtccggaccc
     2581 gtcgagatga tgtcgatggg agcacccacc gtctcctcga tgcgggccag atacgcccgc
     2641 gcattggcag gcaggtcatc caggcgccgg atgcccaggg tggactccga ccagccgtcc
     2701 acctcttcgt acacgggcct gatttccgag taatactcgc tggcaaaccg cggcgtgccg
     2761 ctcgtgccgt ccgggccctc gtaacccacg cagatgcaga tactctccag accgtcgagc
     2821 acgtccagct tggtcaggca gagcccggag atgctgttga tctgcacggc ctgccgcagg
     2881 gccacggcat cgaaccagcc gcaccggcgc ggcctgcccg tggtggaccc gaactcgttg
     2941 ccgcgggtcg caagccgctc gccaacctca tcgaagagtt ccgtcggaaa cggtcccgag
     3001 cccacgcggg tggaatatgc cttcgtgatg cccaggatgt aatccagata tcgaggtccg
     3061 aagccactgc ccaccgcggt cccgcccgcg gtcgtgttgg aggaggtcac gaacggatac
     3121 gtgcccagat caatgtcgag gagcgcgccc tgggctccct cgaacagcag attggctccg
     3181 ctttcgcgaa tctcgtgcag cagcggaatg atgtccgcca ccatgggacg aatctgctcg
     3241 gcaaccgccg cgcactcatc cagggtctgc gcgtagtcca cggtctccga gccgtagtac
     3301 cgctccagca tgaagttgtg atactccatg acctcggcca gcttggccgc gaactcctgc
     3361 cagtagaaaa gatcgccaag acgaacgccg cgtcgcgccg ctttgtcctc gtaggcaggg
     3421 cctatgccgc gcccggtcgt gccgatcttc tgattacccc tggcttcctc ccgggcgaga
     3481 tcgagcttga cgtggtacgg caggatgagc gggcaggcgg ggcttatccg gagccgctcg
     3541 cgcacgggca cgccacgggc ctcgagaccg gacacctcgc tgagcagcgc gttcggctcc
     3601 aggacgacgc cgttaccgat gaagcactgc acctcgcgac gcagaatgcc cgagggaatc
     3661 aggtgcagga ccgtcttctc gccctctatg accagggtgt ggccggcgtt gtgaccaccc
     3721 tggaaacgga ccacggccgc tacctgatcg gtgaggagat cgacgatctt gcctttgccc
     3781 tcgtcccccc actgggtgcc catgacgact acgttgcggc ccatatcagc cctcgttccg
     3841 cccgagcctt tccactttcc ataccccaga gacgtacgaa agctgacgat cgcagcccga
     3901 aggcgccccg tcttcgtcgt ccagcgccac caccaccctc tctccagccg cacgcaggcg
     3961 ccggacctcc gcagccagcg cggcagcgac atcgccgccg aggctgccgg tccagggcgc
     4021 ccagacggcc tggccgacgg tccctgcccc ggcaagcccg atgagctggc gcaggttcac
     4081 gtcgaagccg gtcgcgggcc ggctgcggcc gaacggcgcg ccgattccgt cgtagcgtcc
     4141 gcccctggcg atcgcgcggc cctgatccgc ctggtaggcg gagaacaccg gcccgttgtg
     4201 ataaccgtac ccggcgagct ccgccagatc gtagcgcact tccaccgccg ggttgcggac
     4261 cagcaccgcc tccgccagct cctccagagc atccaccgcg gccaccgcgc catccggccc
     4321 gccagccaac gccgcgcgcg cctcccggag tatctcgcgg ccgcccatga gggtcggcag
     4381 ccgctctaaa agcgccccgt ccgagcgctc gccgatcagc tcaccgatgt ctgcctcgga
     4441 cttgctctgc acggcagcga acagctgccg ctcggtgaac gcatccagac ccagcggtgc
     4501 gaccagactc aggtagatcc ccatgtgtcc gagcacgagc accggggctt cgacgcccgt
     4561 gcgctcgaga atttccagca gcaggcagat cacttccacg tcggccgcga gcccgggcgc
     4621 gccgaacacc tcggcgcctg ccttgagcgg aacccgggaa tccagcgccg ccgccgggtt
     4681 ggcgagaacc acggggcccg cgtagcaaag cctctgcacc ccctcgctcg gacggctgtg
     4741 ggcgtcgatc cgaaccgcct gggccgtcat gtctgcgcga acgcccatca ggcgtccgct
     4801 gcgctggtcg accaccttca gcgtctgcag atcgagatcg tcctggtgac cgagcccccc
     4861 atccggacct gcgccgacga gaagggcatc gaggtactcg accaccggag gctcgacgta
     4921 gtcgaagccc cagctgttga acacgtcgag cacctgacgg cgcacctgct ccagcgccca
     4981 cgcctcaggc ggcagtagct cgtccacccc gtcgggcaag cgccattttc ggttgaccat
     5041 ggatctgacg tccaatcagc ggaccatttc agccggaacg tttagcccgg cttaggaatc
     5101 gttggcgaga tacttgaaga actcgctcgt gggatccagc accaggatgt cgctcttgtc
     5161 cttgaacacc ttcgcgtagg cattgatgct gcgatagaac tggtagaact cgggatcccg
     5221 attgtacgca ctggcatata tgttagccga gcgcgcgtcc ccgtcacccc gtatctgctc
     5281 cgaactccgg taggcctcgg cttcgatgat caccgtctga cgctcagcgt cagcacgaat
     5341 gccgagcgcg agctcgcgac cctgagcgcg atactggcgc gcctcgatct gccgttccga
     5401 gttcattcga tcgtacacgg agctcgagac gtccgggggg aggtcgatgc gcttgacccg
     5461 cacgtcgatc acctcgatgc ccacggctcc gctcatgacc gcattgaggt cccccagcag
     5521 ctcctgcatg agctggtcgc gctcgccgga gaccacctcg tgcatgtcgc gacggctgat
     5581 ctggtttcgc agcccttcgt tgacccgctc ctggagcact cgctggccgc gccgctcgtc
     5641 gaacgaggtg cccgtgtagt aacgctgcac gtcgacgatg cgccacttgg cgaaggagtc
     5701 gacgatgagg ggcttcttct ccagcgtgta gtaggtttct ggcacggagt ccagcgtcag
     5761 cacgcgtccg tcggcctttt tgacctcctc ggcgatgggc atcttgaagt gcagcccggg
     5821 cgggacatcc gcctggctga ccgcgccgaa gcggagcagg atggcgcgtt cggtctcctg
     5881 caccgtgtag acgctgttca tcagcagcag cacggcgatg atggctatga cgacgataat
     5941 gagattcttg agattcatgt cggatcctat cggctttccc gaatccgccc gctggcgatg
     6001 cgttcattga cttcacgaag aatggcgtct gcgatgcttc tgaccgattc gtccgtggcc
     6061 aggccggcca ccgggctccc ggtctgcccg agcctgtcga gaggcagata caggagattg
     6121 ttgcctccct cgacgtcgat catgatcttg ctcgatcggc tgagcaccga ctccatggca
     6181 tcaatgtaga gccggtcgcg ggtcacttcc ttggcgaggc tgtactcggt gagcagcttc
     6241 tcgaaccgct ccgcctcacc ctcggcgcga gcaatcacct gatcgcggta ggcgttggcc
     6301 tgctcgatct gcttctgcgc ttcacccctg gcctcgggaa tgatgctttc cgcgtaagcg
     6361 ttggcttcgt tgatgacgcg ctgctcgtcc tccttggcct tctggacgtc gttgaacgcg
     6421 tcgcgcacct ggttgggcgg cgcgctctcg tcgatgttca ccttgctcac gagaatgccc
     6481 gtgccgtagg tatccagata ggtctgaagc ctgtcctgga cctcggccgc gatggccgcc
     6541 cggcccgcgg tgatgacgtc gtccatcaag gagcttccca cgacgtggcg cagcgcgctt
     6601 tccacggcat gatcgaggct gttctcgggg tcgcggacca tgaccagaaa tttcagcggg
     6661 tcgttgacca cgtactgaac ggtcaggctg acgtcgacga tgttctcgtc ctcggtcagc
     6721 atcagggcct gatgattctt cgagttcacc ctggtgacgt tgaccttgcg gaccacgtcg
     6781 atgatggggg gattccagtg cagtcccggg aggacgatgt cctccttggc cgccccgaac
     6841 cggaacacca cccctcgctc ctgctcgtcg agctgataca ggccgaacag accgtaggct
     6901 acggccgcga cgacgagcac aatgcccagc atccccgcat tcaaaccgct gccatcgcca
     6961 ccactgccgc cgcgcccacc cccaaaaatg gaggcaagct gggcctgcag cttcttgaaa
     7021 gcttcatcca gatcaggtgg gccctggtct ccgcggcttc cccacgggtc cttgtcacct
     7081 ccacccggct cgttccaggc catctcgtgg tgctccgtta aagattaatt gggcaggggc
     7141 aagattctag ggacggcgtc attgacctgc aacagaaccc tgggcgtgcg gcccaggcgc
     7201 tccagcagat cggcgtccgc acgaacccgc agcgcaaccc gtccgtcctc ggtgatctct
     7261 tcgccaagca cggcgcccat tcgatacagc caggcgcgcg tcttgccatc atcggcgggc
     7321 agaacca
//
LOCUS       DBNHLM010000101        13521 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_254010, whole genome shotgun sequence.
ACCESSION   DBNHLM010000101 DBNHLM010000000
VERSION     DBNHLM010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13521
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_254010"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /gene="glsA"
                     /locus_tag="AC16QF_04700"
     CDS             <1..1488
                     /gene="glsA"
                     /locus_tag="AC16QF_04700"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HIR6447884.1"
                     /translation="IQYIYEQVASDRSGANADYIPILRDADPERWGVAVCTVDGQRLA
                     IGDVDVYHSVQSVSKPLTYAYALAREGEEFTHQFVGVEPSGRPFNALDLLPDMRPYNP
                     CVNAGAIMVAGVVASGAPDLDAREITTQLMELWAGLCGRCAPVRFSEETMLSERASAY
                     NNFAIAYLLQGRRGLPRDVDLHKMLDVYFSCCSMEMTARMLSVAAATLANGGVCPING
                     RQVLSTDIVKRTISVMQAAGMYDNAGVFTLEVGLPAKSGVAGSVMVVVPNLLGFATFS
                     PRLDSYGNSVRGVSFCRQLVERYSVHMYDNLSGGRSGCKRDPRVSRQQRKQRDLSDLR
                     WGIANGDHTALRVRDMILHCMVDISLADGELQAAELAIMESVHSELIGEPALSGALEA
                     LARSRADTAAAWRDGSQNGGDVGPARSPFDRLLVELGEQSSLIDDQAREIILETAFQV
                     ACADGIIEMEEQEKLRAVAGALGIHEAVLELEINAFRLSQSAQPV"
     gene            complement(1547..2221)
                     /locus_tag="AC16QF_04705"
     CDS             complement(1547..2221)
                     /locus_tag="AC16QF_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447885.1"
                     /translation="MKRTMFALGAALLLSGSLAQAAALNVTIWQAIPGKSAAMMESAM
                     AAKAIQEKLGASVTIALDNTGRLHYGVGGFENWGAWAKWVEKLQDSEDWAAWQQRSGA
                     DPAAIQEENFILNTVPGTDSPVGRDDLGSVYQVFIWDPSGTNVAPLVERALQAAAIHA
                     KAGIAVGVSVDQHLRMHYVMNFKDWADWAKLQDNPIEEFEQFMLRNAANPVGDLVEVY
                     TVNRLP"
     gene            2436..4385
                     /locus_tag="AC16QF_04710"
     CDS             2436..4385
                     /locus_tag="AC16QF_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR6447886.1"
                     /translation="MTSRWWLLAGGILMLLLVACGQRSQPDAPADAAAEPAGEARGGD
                     FDMADTSSVGGRVYLESCATCHEGGVDRAPQRAMLLLMSPESIHRALTKGVMRPQAAA
                     LSEADKVAVAEFLSRSTMGDTQALAAPPGCAGESAGFDYAEPPVFSGWGLDPASTHAI
                     PAETAGLTPANVGKLKLKWAFGFPGALRARSAPALAGGAIFVGSHGGTVYALDRETGC
                     ARWTFDASAEVRTGIVISPWAAGDTSAEPLAFFGDLIGNLYALNAVTGGLVWRARPDE
                     HPNTTLTGTPTVHEGTLYVPVSSLEVVPAREPDYECCSFRGSVVAYDARTGTEKWQTF
                     TVAEEPRPQGVNAAGTQNFGPSGAPIWNSPAIDVRRGQLTVGTGENYSSPADGGSDAI
                     FAMDLVTGKVNWVFQATAGDAWNVACGSGDRTNCPVEDGPDFDFGAGTLLALDVAGRD
                     LVIAGQKSGVVHALDADTGELLWQTKVGRGGVHAGVYFGMAAHGERLFVPISDTDDGR
                     EYAEPPRPGMYALDMRTGEFVWRVPAVNVCREDQELCQPGYAQAISATPELVIAGSID
                     GHVRIFSAATGAVVWDFDTATDFAAVGGGTARGGGFAGAAAPIAYQGQLIISSGYGFA
                     GKMPGNALLVFEVAAARAGGSLPIE"
     gene            4759..6111
                     /locus_tag="AC16QF_04715"
     CDS             4759..6111
                     /locus_tag="AC16QF_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6447887.1"
                     /translation="MAAQGSRIPRGKLLSYSAAEFPMSMASTPVSLFIPAFYTQDLGL
                     GMAAVGGILMLSRFWDVVTDPVIGYLTDRTRSPIGRRKPWIIASVPIVMISIYNLFFP
                     PEDADETFLLIWIMALWFGWTLFNIPYFAWGAELSTSYTERTRVTGWRTTAGLLGTLI
                     AIAIPAASQALFGWGGRSGEMIYMIGIGVLLLTPLLVVPAVAFVPERSDFVPAQMNVV
                     EGLRIMWSNGPFRRLLFAFVFGSLAVALSAPLFVIFISHVIEDPTAAPRVVLGYYAGN
                     LLGIPFWVWLARKTDKHITWLFSISLMGLMFPQFLLLGPGDTWLAAFLLFMVGLGGGN
                     MAVVPTSMKADVIDLDSLESSEDRAGLFFAAWSTATKMVGALGVGISLPALAYLGFDP
                     TVVNGPDELFSLRAWFSLVPVLFYLASAALIVGYPITRARHAQIRSELEARKLSGDTG
                     "
     gene            complement(6130..6336)
                     /locus_tag="AC16QF_04720"
     CDS             complement(6130..6336)
                     /locus_tag="AC16QF_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447888.1"
                     /translation="MVSASGGSLTGQHALVTGGGSGIGLVDTEIAQGLFGTEVVLEDY
                     LACMPLGRTGVVEDVTLGRFDSLK"
     gene            complement(6394..9465)
                     /locus_tag="AC16QF_04725"
     CDS             complement(6394..9465)
                     /locus_tag="AC16QF_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6447889.1"
                     /translation="MRFTDVFIQRPVVAIVLGAVMLLLGLQASTQLSLRQFPEVEKSV
                     IFVRTVYPGASARTVQGFVTAPLQRTIAAAKGVEYVTSESNPGSSEIEVHVRLGENST
                     EVLSEVITKVNEARFELPRDVEDPVVTNRTGGDAMMYLALLSDQMSVQQVADYGLRTI
                     QPVLNTVEGVGEARLLGGGLFAMRIWLNPARMAAFGVTATDINDAIRRDNYVSAAGMT
                     HGELVRASVDAQTDVQQPEKFAEIVVRQDEDRRVRLGDVATVELASETYDFAVYSSGK
                     ETVFIAINQAPGSNPLEVAARVKEKVAELEAGLPADLTIFMDSDLSKYIGEALKEVAA
                     TLIEASVIVVLVILLFLGSLRVVAIPIVAIPLSLTTVLFLIWAMGFSINLLTLLAMVI
                     AIGLVVDDAIVVVENVHRHIEDGETPYDAAIVGARQVALPVIAMTLTLVAVYLPIGFL
                     GGLTGVLFSEFALTLAGAVLVSGVVALVLSPMMCARLHKDHAHQGRAADWLDARFDRL
                     HRGYERLLSYCLDNRGAVMLFAGAIFLSLPALYLLAQKELAPEEDSGSIFAVAVPPDY
                     GNLEYTTLFLDQMVQAWRTVPEVTHSWQVNEPGSVFGGLELKPWSERERTLEEVRQEV
                     QEKYARISGLEIFTFAGGGLPGADSGLPVQFVISSNADYRELDRVSEEVLQLARSSGM
                     FAFVNKDLRYSRPEVNVRINRELAARLGISMEEIGETLQVMLGEAETNRFGVEGRSYK
                     VIPQADRGFRLTKEWLERYYLRTSNGELVPLSTVISLYQSVEPNALKQFQQLNAVMIQ
                     GFVLPPNSLGDALAYLEGALKEVAPTGYRVGYQGESRRYVQETQGFLRLFATSLIFIY
                     LVLAAQFNSFRDPFIVLISVPLSIFGAVVPLALGFVTLNIYTQVGLLTLIGLISKHGI
                     LIVDFANGRIAEGVDRRQAVIEAAGLRLRPILMTTFATVLGVVPLLNAFGAGANSRFA
                     IGLMIAAGMSVGTLFTLFVLPTFCLLVGRQEGEQPTHHPKSARYVLSEQRR"
     gene            complement(9468..10538)
                     /locus_tag="AC16QF_04730"
     CDS             complement(9468..10538)
                     /locus_tag="AC16QF_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6447890.1"
                     /translation="MKAYAIVAALLIAIFGAIGGYLYQKFSALASMDFSPPPVTIATS
                     VAQRETWDERLNAVGTVQAVRGVDLTSETSGEIIRINFDSGDAVEAGQLLVVLNDEIE
                     QASRRNQIASLELAEILFERDRALIAQKSIPQSQFDRSRADLERARAQLAETEARLAN
                     KRIEAPFGGTMGIRRVDLGDYLSPGAVIAALQDHSELEIDFTVPARYAPKLRAGLKVA
                     VVVDAFPDREFSAEVVAVDSRIDPSTRNVLLRARLDGAGGLLPGMFATLTVDLGERVD
                     LVTIPETAMTYSLQGNTVYVIEDTEDGGKTAVARVVMSGPVRGGRVAVRSGLQPGEQV
                     VTVGQNKLFRGVRVVIDDAVRF"
     gene            11006..11746
                     /locus_tag="AC16QF_04735"
     CDS             11006..11746
                     /locus_tag="AC16QF_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447891.1"
                     /translation="MFVLLAVPGFNASADSHASPPVQPALEAFFCNYNPGKDRDDLDS
                     ATNFYMKQAKKAGFTTPNAFLWTHNKGTAPADIVWMNVHESLVAYGASDDAAAASSEM
                     ADVNDRYDSVATCQAGLGIGTPIITPDPAAAGLEMSTLAAYACTFRHGVGPDALVDLR
                     NHIRETNEGMGDAGLAAAFQIEPMTGGANASDVVFVAVAESTEAWAANLAMLNSTPAG
                     QSLVRHFNMVVDCDMNLWWSEQVVGGEG"
     gene            complement(12060..>13521)
                     /gene="rpoD"
                     /locus_tag="AC16QF_04740"
     CDS             complement(12060..>13521)
                     /gene="rpoD"
                     /locus_tag="AC16QF_04740"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIR6447892.1"
                     /translation="LLTREGEVAIAKRIEEGMREVLAGAARFPEGVQHLLDQYDRAVA
                     EGKLSDVLVGYLDPVEGIPLPPKFDPANADKNDQDVYKGPDPVEADRRFAALRGAYEK
                     AQSSEAGKRRTSPKAQSDLEQVSKALSSLKLAPRHYQALVDGSRELIDEVRVHERAIM
                     SCCVRNAGMPREVFLKEFPGNETSVAWLNRHIKDQHRYSKTLSAEMPAIQHTQKRLKA
                     LTKRTGLSIGETREISRRMALGEAKMSRAKKEMVEANLRLVISIAKKYANRGLPFLDL
                     IQEGNTGLMRAVDKFEYRRGYKFSTYATWWIRQAISRAIADQARTIRVPVHMIELISK
                     FHRATRQLLQELGREPSPSEIGALMELSEEQVDRVVKISKHALSLEIPVGDDGDQHLG
                     DLIEDADTASPADLAIDERLKEATQAILGDLTVREAKILRMRFGIDMNTDHTLDEVGK
                     QFNVTRERIRQIEAKALRKLRHPKFSEQLLGFLRDD"
BASE COUNT         2349 a         4321 c         4239 g         2612 t
ORIGIN      
        1 attcagtaca tctacgaaca ggtggccagc gaccgcagcg gtgccaatgc cgactacatc
       61 ccgatcctgc gggatgccga tccggaacgc tggggcgtgg cggtctgcac ggtcgacggt
      121 cagcgcctgg cgatcggtga tgtggacgtc taccactccg tccagtccgt ctccaagccg
      181 ctcacctacg cctacgcgct ggcccgggag ggagaggagt tcacccacca gttcgtcggc
      241 gtcgaaccgt ccggaaggcc cttcaatgcc ctggatctct tgccggacat gcggccttac
      301 aacccctgcg tcaatgctgg cgcgatcatg gtggctggcg tggtggcgtc cggcgcgccg
      361 gatctggatg cccgtgagat caccacgcaa ctgatggagc tctgggccgg cctctgtggc
      421 cggtgcgcgc cggtacgctt cagcgaagag accatgcttt ccgagcgcgc cagcgcttac
      481 aacaatttcg ccatcgccta tttgctgcag ggccgtcggg gcctgccccg cgatgtagat
      541 ctgcacaaga tgctggatgt ctacttttct tgctgcagca tggagatgac cgcacgcatg
      601 ctgtccgtag cggcggctac gctggcgaac gggggcgtat gtcccatcaa cggacgccag
      661 gtgctctcca ccgacatcgt caagaggacg atctcggtga tgcaggcggc aggcatgtac
      721 gacaatgccg gcgtgttcac gttggaggtg ggcctgccgg ccaaatccgg cgtcgccgga
      781 tctgtcatgg tcgtggtgcc caacctgctg ggattcgcca ccttctcacc gcggttggat
      841 tcctacggga attcggtgcg gggcgtcagt ttctgccggc agctggtcga acggtacagt
      901 gtccacatgt acgacaatct gtccggcggc cgttcgggct gcaagcgtga tccgcgcgtg
      961 tccagacaac agcgcaagca gcgggatctc tccgatctgc gctggggcat cgccaatggt
     1021 gatcacaccg ccctgcgggt gcgtgacatg atcctgcact gcatggtgga tatcagcctg
     1081 gcggacggcg agctgcaggc agccgaactg gccatcatgg agtccgttca tagcgaactg
     1141 atcggtgagc cagcgctgtc gggtgccttg gaagcgcttg cccgttcgcg ggcagacaca
     1201 gctgcggcct ggcgcgatgg ttcgcagaac ggcggtgatg tcggcccggc gcgttcgccg
     1261 ttcgatcggc tgctcgtcga gctcggcgag caatccagcc tgatcgatga ccaggcgcgc
     1321 gagatcattc tcgaaaccgc ttttcaggtg gcctgcgccg acggcatcat cgaaatggaa
     1381 gagcaggaga agctacgagc cgtcgccggc gccctgggca tccacgaagc ggtgctggaa
     1441 ctggagatca acgcgttccg gctctcccag agcgctcagc ccgtttgacg ccgggcggat
     1501 ggcccgctgg atgtccgccc gacgggcgga catccgagcg aaccgactac ggcaggcgat
     1561 tgaccgtgta cacctcgacc aggtcgccga ccggattggc cgcgttgcgg agcatgaact
     1621 gctcgaactc ctcgatgggg ttgtcctgca gcttggccca gtctgcccag tccttgaagt
     1681 tcatcacgta gtgcatccgc aggtgctgat cgacgctgac gcccaccgcg atgccggcct
     1741 ttgcatgaat cgcggccgcc tgaagtgccc gctcaaccag cggcgcgacg ttcgtgccgg
     1801 acgggtccca gatgaatacc tggtatacgc tccccagatc gtcccggccg accggactgt
     1861 cggtgccggg caccgtgttg aggatgaagt tctcttcctg tatggcggca ggatctgcgc
     1921 ccgatctctg ctgccaggct gcccagtcct cactgtcctg gagcttttcg acccacttcg
     1981 cccaggcgcc ccagttctcg aagccgccca ctccgtagtg caggcggccg gtgttgtcca
     2041 gcgcgatcgt gacgctggcc cccagcttct cctggatggc cttcgcagcc atggccgatt
     2101 ccatcatggc tgcagacttc ccgggaatcg cctgccagat cgtcacgttc agggcggctg
     2161 cctgagcgag cgaaccggac agcaacagcg ccgcgccgag cgcaaacatc gtgcgtttca
     2221 ttcgttttgt ccttttggtc gtttgactag ccggtcaggt accggcacac cgaggtacgt
     2281 cgaacttctc cccgcccaga atctagcaca ctcgcccgcc actcagcgcg tcgtttcgtt
     2341 ctcgccatgt tcccggtctc gctctaggat acccgggtct atccatggcg ccctggtgcg
     2401 gctcgctggc cgcgccctca tccctgggag gaagcgtgac ttcaagatgg tggctgctgg
     2461 ccggcgggat cctgatgctg ctgctcgtgg cctgcggaca gcgttcgcaa ccggacgccc
     2521 cggccgatgc cgccgcggag ccggccggcg aggcgcgcgg cggcgacttc gacatggccg
     2581 atacctccag cgtgggcggg cgggtttacc tcgagtcgtg cgcgacgtgt cacgaagggg
     2641 gcgtcgaccg ggcgccgcag cgggccatgc tgctgctcat gtcgcccgaa tcgatccacc
     2701 gcgctctgac caagggcgtg atgcgccctc aggcagccgc cctgtccgag gcggataagg
     2761 ttgccgttgc cgagtttctc tcgcgcagca ccatgggtga cacccaggcc ctggcagcgc
     2821 caccaggctg cgcgggcgag agcgccgggt tcgactacgc cgagccgccc gtcttctcgg
     2881 gttggggact ggatccggca agcacccacg ccatcccggc ggaaaccgcc gggctgacgc
     2941 cagccaacgt cggtaagttg aagctcaagt gggcattcgg ctttccgggc gccctgcgtg
     3001 cgcgctcggc gccggcgctg gctggcggtg cgatcttcgt gggcagccat ggcggcaccg
     3061 tctacgcgct cgaccgagag actggctgcg cgcgctggac gttcgatgcc tccgcggaag
     3121 ttcgcaccgg catcgtcatc agcccctggg ccgccggaga tacgtcggcc gagccgctgg
     3181 ccttcttcgg tgatctcatc ggcaacctct atgcgctgaa cgcggtgacc ggcggcctgg
     3241 tctggcgcgc gcggcccgac gaacacccga acaccaccct gaccggcacg cccaccgtgc
     3301 acgaaggaac gctttatgtg cccgtctcct cgctggaggt ggtgccggcc cgggagccgg
     3361 actacgagtg ctgctccttc cgcgggtccg tggtggccta cgacgcccgg accgggaccg
     3421 agaagtggca gacgttcacc gtggccgagg agcccaggcc tcagggcgtc aacgcggcgg
     3481 gaactcagaa cttcggcccc tccggcgcgc ccatctggaa cagcccggcc atcgatgtgc
     3541 ggcgcggaca gctcacggtg ggcaccggcg agaactactc gtctcccgcc gatggcggca
     3601 gcgacgcgat cttcgccatg gaccttgtga ccgggaaggt gaactgggtc tttcaggcca
     3661 ccgccgggga cgcctggaac gtggcctgcg ggtcggggga ccgaacgaac tgcccggtcg
     3721 aggacgggcc cgacttcgac tttggcgcag gcaccctgct ggccctggac gtcgccggtc
     3781 gcgacctggt catagccggt cagaagtcgg gcgtcgtcca tgcgctcgat gcggataccg
     3841 gcgagctgct ctggcagacg aaggtgggcc ggggcggcgt gcacgcgggc gtctacttcg
     3901 gcatggccgc gcacggggag cgtctgttcg tgccgatcag cgataccgat gacggccgcg
     3961 agtatgcgga gcccccgcgg ccggggatgt atgccctgga catgcgcacg ggtgagttcg
     4021 tgtggcgggt gccggcggtc aacgtctgcc gggaggatca ggaactctgt cagcccggtt
     4081 acgcccaggc gatctcggcg actccggagc tggtcattgc cggcagcatc gacggccacg
     4141 ttcgcatctt ctctgcggcg acgggcgccg tggtctggga cttcgacacg gctacggatt
     4201 tcgccgccgt cggcggcggc accgcccgtg gaggcgggtt cgccggcgcc gcggcgccga
     4261 tcgcctatca gggccagctc atcatcagct ccggctacgg attcgccggc aagatgccgg
     4321 gtaacgcgct gcttgtcttc gaggtggcgg ctgcccgggc gggcggaagt ctccccatcg
     4381 agtgacgtct ttccaggttg ggagatcacc ccctcgacgg cattgaactc ggcgaggtgc
     4441 tgaactaacg tcgggacccg ggcagccgcg ggggcacagg gaggagagat ggaattttgg
     4501 accactgcgg tgggcgtgcc ggatgcggtt gcccgggagg cccggggcgc cgaagcgcag
     4561 ggttgggacg gcttggcgta ccttgcgcgc cgaccatgac ccccgatccc tacgtctgcc
     4621 tgacggtggc ggcagaggcg agggggccga attgcgctcc gatcgctcgc tgatcgatcc
     4681 ggggcatccc gtgcggttgt gctctttcgc gaatccggaa ggcaacgtga tagagccggt
     4741 ggaagttggc tagctagggt ggctgcgcag ggatcgcgaa tcccccgggg caagctgctc
     4801 tcctacagcg cggccgagtt tcccatgtcc atggcaagca cgccggtgtc gctgttcatt
     4861 ccggctttct acacccagga cctgggtctg ggcatggccg ccgttggcgg catcctcatg
     4921 ctctcccggt tctgggacgt ggtgaccgat cccgtcatcg gctacctcac ggaccggacc
     4981 aggagcccca tcggccggcg caagccctgg atcattgcgt ccgtgcccat cgtgatgatc
     5041 tccatctaca acctgttctt tcctccggaa gatgcagacg agacctttct gctgatctgg
     5101 atcatggcgc tttggttcgg ctggaccctg ttcaacattc cctacttcgc ctggggcgcc
     5161 gagctctcca cgtcctacac cgagcgtacc cgggtgacgg gttggcgtac cacggccggg
     5221 ttgctgggca cgctgatcgc gatcgccatt ccggccgcaa gccaggcgtt gttcggttgg
     5281 ggcggacgga gcggcgagat gatctacatg attggtatcg gcgtgctgct gctcaccccg
     5341 ttgcttgtgg tgccggcagt ggccttcgtg ccggagagga gcgatttcgt ccctgcgcag
     5401 atgaatgtcg tcgaggggct gaggatcatg tggagcaacg gtcctttccg ccgcctgctg
     5461 ttcgccttcg tcttcggctc gcttgcggtg gcgttgtccg caccgctgtt cgtgatcttc
     5521 atttctcatg tgatcgaaga tccaacagca gcgcccaggg tggtgttggg gtattacgcg
     5581 ggaaacctgc tcggcatccc gttctgggtg tggctggcgc gaaaaacgga caaacacata
     5641 acctggctgt tctcgatctc gttgatggga ttgatgtttc cccagttcct cctgctgggt
     5701 cccggcgata cttggttggc cgcgttcctg ctgttcatgg tcggtttggg cggcggcaac
     5761 atggccgtgg tgcctacctc catgaaagcc gacgtgatcg atctcgacag cctggagagc
     5821 agcgaggaca gggcggggct gtttttcgcc gcctggtcca ccgccaccaa gatggtgggg
     5881 gctcttggtg tcgggatctc gctaccggcg cttgcctatc tcggctttga tccgaccgtg
     5941 gtgaatgggc ctgacgagct attttcgctg cgtgcctggt tttctctggt tccggtgctg
     6001 ttctatcttg cctctgccgc gctgatcgtc ggctatccca tcacgcgtgc gcgccatgcg
     6061 cagatccgct cggaactcga agcgcgaaag ctgtctgggg ataccggcta ggctgactca
     6121 gactcgtcgt tatttcagag agtcaaagcg gcctagtgtg acgtcctcca ccaccccggt
     6181 gcgcccgaga ggcatgcagg ccagatagtc ttccagcacg acctcggtgc cgaacagccc
     6241 ctgggcgatc tccgtgtcca ccagcccgat tccgctgccg ccaccggtaa ccagcgcatg
     6301 ctggccggtc agggatccgc ctgaagctga gaccatggct gtccttcgta gattcccgaa
     6361 tcctcagctc tagcaagagc gtcgaagcct gggctaccgt cgctgctcgc tgagaacata
     6421 tcgagcagat ttcgggtggt gtgttggttg ttcgccctcc tgcctgccaa cgagaaggca
     6481 gaacgtcggc agcacgaaca acgtgaaaag cgttccaacc gacatgcccg cagcaatcat
     6541 caggccaatc gcgaagcgag agtttgctcc ggcaccgaac gcgttgagca gcggcaccac
     6601 ccccagcacc gtggcgaagg tggtcatcag aatcgggcga agcctcaagc ccgccgcctc
     6661 gatcaccgcc tgcctcctgt cgacgccttc cgcaatgcgg ccgttggcga agtcgacgat
     6721 caggatcccg tgcttgctga tcaacccgat cagggtcagc agccccacct gggtgtagat
     6781 gttcagcgtg acgaatccca gcgccagcgg caccaccgcg ccgaagatcg atagcggcac
     6841 gctgatcagc acgatgaagg ggtcccggaa gctgttgaac tgggccgcca gcaccagata
     6901 gatgaagatc agggaagtgg cgaacagccg gagaaatccc tgagtctcct gaacgtatcg
     6961 gcgcgactct ccctggtagc cgacccgata tccggtgggc gccacctctt tgagcgcgcc
     7021 ctccagatac gccagcgcgt cgcccaagct gttgggcggc agaacgaatc cctgaatcat
     7081 cacggcgttg agctgctgga actgtttcag ggcgttgggc tccacggatt ggtacagcga
     7141 gatcaccgtg ctcagcggaa ccaactcgcc gttggacgtg cgcaggtagt aacgctcgag
     7201 ccattccttg gtcagccgga atccccggtc ggcctggggg atcaccttgt agctgcgtcc
     7261 ctccacgcca aaacgattgg tttcagcctc ccccagcatc acctggaggg tctcgccgat
     7321 ttcctccatg gagatcccca gccgtgcggc cagctcgcgg ttgatcctga cgttgacctc
     7381 gggtctggaa tagcgcagat ccttgttcac gaaggcgaac atgccggagc tccttgccag
     7441 ctgcagcact tcctctgaaa cccggtcgag ctcccggtaa tcggcgttgg aagaaatcac
     7501 gaactgcacg ggcaatccgc tgtccgcgcc gggcagacca ccaccggcaa aggtaaaaat
     7561 ctccaggccg ctgatcctgg cgtacttctc ctgtacctcc tgccgcactt cctccagggt
     7621 tctctccctt tccgaccagg gcttgagctc gagacctccg aacaccgagc caggctcgtt
     7681 cacctgccac gaatgggtta cctcgggaac ggttcgccat gcctgcacca tctgatccag
     7741 aaagagggtg gtgtactcca ggttgccgta gtcgggtgga acggccacgg caaagatgct
     7801 tccgctgtct tcctccggtg ccagctcctt ctgggcaagc agatagagcg ccggcaggct
     7861 gagaaagatc gcgccggcaa acagcatgac ggcgccccgg ttatccaggc agtacgacaa
     7921 caggcgctcg tagccccgat gcaggcggtc gaatcgggca tcgagccagt cagccgctct
     7981 gccctggtgc gcgtgatcct tgtgcagccg cgcgcacatc atcgggctca gcacgagcgc
     8041 aacgacgccc gacaccagca cggcgccggc cagagtcagc gcaaactcgc tgaacagcac
     8101 ccccgtcagg ccgcccagga aaccgatcgg caggtaaacc gcaaccagcg tgagcgtcat
     8161 ggcgatgacc ggcaaggcaa cctgacgtgc gccgacgatg gccgcgtcgt acggcgtctc
     8221 gccatcttca atgtgccgat gcacgttctc caccacgacg atggcatcgt ccaccaccag
     8281 cccgatggct atgaccatgg ccagcagcgt cagcaggttg atggagaagc ccattgccca
     8341 gatcaggaac agcacggtgg tcagcgaaag gggaatcgca acgatgggaa tcgccacgac
     8401 gcgaagcgat cccaagaaca gcagaatcac caggacgacg atgacggagg cttcgatgag
     8461 ggtcgcggcc acctccttca gcgcttcccc gatgtacttg gacagatcgg aatccatgaa
     8521 gatcgtcagg tctgcgggca gaccggcttc cagctcggcg actttctcct tgacgcgtgc
     8581 agccacctcc agaggattgg agcccggcgc ttggttgatc gcgatgaaga ccgtttcctt
     8641 gccgctggag taaacggcga agtcgtaggt ttcgctggcg agctccacgg tagccacgtc
     8701 accgagccgc acgcgcctat cctcgtcctg gcggaccacg atctcggcaa acttctccgg
     8761 ctgctgcaca tcggtctgcg catctacgct ggcccgaacc agctcgccgt gggtcatgcc
     8821 tgcagcggaa acgtagttgt cgcgccgaat cgcatcgttg atgtccgttg ccgtgacgcc
     8881 gaaggccgcc atccgggccg ggtttagcca gatccgcatg gcgaacaggc cgccgccgag
     8941 caggcgcgcc tcaccgacac cctccacggt gttcaggacc ggctgaatcg tgcgaaggcc
     9001 gtaatcggcc acctgctgga ccgacatctg atcgctcagc aatgccagat acatcatggc
     9061 gtccccgccg gtccggttcg tgaccaccgg gtcctccacg tcccggggaa gctcgaagcg
     9121 cgcttcgttg actttcgtga tcacctcgga gagcacttcc gtggagtttt cgccgaggcg
     9181 gacgtgcacc tcgatctcgg acgagcccgg gttgctttcc gacgtcacgt actccacgcc
     9241 tttcgcggca gcgatggttc gttgcagggg agccgtcacg aacccctgca ccgtgcgtgc
     9301 gctggcgccg gggtagacgg tgcgaacgaa gatgacgctt ttctcgacct cgggaaactg
     9361 ccgcagagac aattgcgtgc tcgcctgaag cccgagcaga agcatgaccg cccccaacac
     9421 gatcgcgacc acgggccgct ggatgaacac gtcggtgaag cgcatggcta gaatcgcacc
     9481 gcgtcgtcga tgacgacccg aacaccccga aacagcttgt tctgccccac ggtgacgacc
     9541 tgctcgcccg gctgcagacc ggaccgaacg gctaccctgc cgcctctgac cggtcccgac
     9601 atcacgacgc gggcaaccgc cgtcttgccg ccatcctcgg tatcctcgat gacgtacacc
     9661 gtgttgccct gcagcgagta agtcatggcg gtctccggga tcgtcaccag atcgacccgt
     9721 tcgccaaggt ctaccgtgag cgtggcgaac atgccgggca gaaggccccc ggcgccatcg
     9781 agccgggccc ggagcagcac gttgcgggtc gacgggtcga tcctcgaatc aaccgcgacc
     9841 acctcagcgc tgaattctct gtccggaaac gcgtccacga ccaccgcgac cttcaggcca
     9901 gctctcagct tgggtgcgta tctcgcggga accgtgaagt cgatttcgag ctcgctgtga
     9961 tcctgcagcg ccgcgatgac ggcgccggga gagaggtagt cgccgaggtc gaccctgcgg
    10021 atacccatgg tgccaccgaa gggcgcctct atacgcttgt tggcaagcct tgcctccgtt
    10081 tccgcgagct gcgcgcgagc acgctcgagg tcggcgcgtg atctgtcgaa ctgagactga
    10141 ggtatcgact tctgcgcgat gagcgcccga tcccgttcga agagaatctc tgcgagttcc
    10201 aggctggcaa tctgattgcg gcggcttgcc tgctctatct cgtcgttgag cacgacgagc
    10261 agctgtccgg cttcaacggc gtcgccggaa tcgaagttga ttcgaatgat ctcgccgctc
    10321 gtctcggacg tcagatcgac accccgaacg gcctgcaccg taccgacggc gttcaggcgc
    10381 tcatcccacg tttcgcgctg cgccaccgag gtcgcaatgg tgacgggcgg aggggagaag
    10441 tccatggacg ccaaagcgct gaacttctga tacagatatc ccccgatcgc cccgaagatg
    10501 gcaatcagca aggcagcgac gattgcatag gctttcaaag gagatctgcc cccaaccaga
    10561 cgtccatgga cattgtgcat cgcaattctc tgctttcaaa ccgaccgtgc ggcgcagcct
    10621 ccacaaacca gccgctgcct gctcgtcttt gtgcctcgag caggctgacg ctgtcgccgt
    10681 ggtcacgatc gaagatcaca ccgatatcag cagtgccgta tcgtcaccgt agttcagtga
    10741 atcgcacgtg caagctgatc aacgatcctc ctaggttccg cgggcgttgt ttacatcatc
    10801 agcttaacgc cgcttggcgc gcttcggctc ggtggccttt tgcggcaaat tgctggagac
    10861 tatcgtgggg ggaagctgat gagtggcagg tgcttcgcac ttgctctgat tgttgtgacc
    10921 ctcatgagcg aaaacagacc agaataagag gaacgcaaca gtgttaaaaa atatgactca
    10981 attgcgccga gggctcgtgc ctggcgtgtt cgtgctgctc gccgtccccg gcttcaacgc
    11041 cagcgcagat tctcacgctt cgccgcctgt ccagcccgcc ctggaagcat ttttctgcaa
    11101 ctacaacccg ggaaaagatc gcgacgatct cgactcggcc accaacttct acatgaaaca
    11161 ggcaaagaaa gccggtttca cgacgcccaa cgcgtttttg tggacccata acaaaggaac
    11221 ggctcctgcg gacatcgtat ggatgaacgt gcatgaaagc cttgttgcct acggtgcgtc
    11281 agacgatgcg gccgcggcgt ccagcgagat ggccgatgtc aatgatcggt acgattcggt
    11341 ggcgacctgc caggccggcc tgggaattgg cactccgatc atcacgcccg atcccgcggc
    11401 ggccggcctg gagatgtcca cgctcgccgc gtacgcgtgt actttccgcc atggggtcgg
    11461 acctgacgcc ctggtggatc ttcgcaacca tatccgggag acgaacgaag gaatgggtga
    11521 tgcgggcttg gccgcggcgt tccagatcga gccgatgacc gggggcgcga acgcgtccga
    11581 tgtggttttc gtggctgtcg cagaaagcac cgaagcgtgg gccgccaacc tggcgatgct
    11641 caacagcacg cccgcagggc aatcgctggt acgtcatttc aacatggtcg tcgactgcga
    11701 catgaacctg tggtggtccg agcaggtcgt aggcggagag ggctgaagca ccgccggatc
    11761 atcgtccatc agtaccggcc tcctcggggg ccggtcgtct tgacggacag cgctgcaaga
    11821 gccctgtgtt cgctcctgaa cgggggagcg tcggagctat tgatatctct ttcgaccagc
    11881 gggaagacgc ctattcccac gagcacttcg ccgtgcacga tcacgccatt ttgggtttcc
    11941 tcaacacgcc cggcggcggc ttgagaacca gaagaacgga agcccagcgt tacgctgctt
    12001 gacgggtcgc cagcaccgga tgtgtgagga gacgtcgtca gaccgctccc tgaagtattc
    12061 tagtcgtccc ggaggaagcc gagcagctgc tcggagaatt tcgggtggcg gagcttgcgg
    12121 agcgctttgg cctcgatctg acgaatgcgc tcacgggtca cgttgaactg tttcccaacc
    12181 tcgtcgagcg tgtggtcggt gttcatatcg atgccgaatc gcatgcgtag tattttggct
    12241 tcgcgtacgg tcaggtcgcc aaggattgct tgggtcgctt ccttcagccg ctcgtcgatc
    12301 gccaggtctg caggtgatgc ggtgtctgcg tcttcgatca gatcccccag atgctgatcg
    12361 ccgtcgtccc cgaccgggat ttccagagat agcgcgtgct tcgagatctt cacgacgcga
    12421 tcgacctgct cttcggacag ctccatcaga gcaccgatct cgcttggcga tggctcacgc
    12481 ccgagctcct ggagcagttg acgcgtggcg cggtggaatt tggagatgag ctcgatcatg
    12541 tgcacgggaa ctctgatggt gcgtgcctga tcggcgatcg ctcggctgat agcctgccga
    12601 atccaccagg tcgcataggt cgagaacttg tagccgcgcc ggtactcgaa cttgtccacc
    12661 gcccgcatta gccccgtgtt gccctcctga atgagatcga ggaatggcag tccgcggttc
    12721 gcgtacttct tggcgatgga aatgaccagg cggagattcg cctcgaccat ctctttcttc
    12781 gctctgctca tcttcgcttc accgagtgcc atgcggcggc tgatttcccg tgtctccccg
    12841 atcgaaagac cggtcctctt ggtgagagcc ttgagccttt tctgcgtgtg ttggatcgcg
    12901 ggcatctccg cgctgagtgt cttgctgtaa cggtgctggt ctttgatgtg acgattgagc
    12961 caggcaaccg atgtctcgtt gcccggaaat tccttcagga agacctcgcg cggcattcca
    13021 gcattccgca cgcagcagct catgatcgcg cgctcatgta cacgcacctc atcaatgagc
    13081 tcgcggctgc catcgaccag agcttggtaa tggcgtggcg cgagcttcag cgaggataga
    13141 gccttgctga cctgttcgag atcgctttgc gctttcggtg atgtccgccg cttgcccgcc
    13201 tctgaacttt gggccttctc ataggccccg cgaagcgctg caaatcgacg atctgcttcc
    13261 accggatccg gacctttgta tacgtcctga tcgttcttgt cagcgtttgc agggtcgaac
    13321 ttggggggca gtgggattcc ttcgaccgga tcgagataac cgactagaac gtctgatagt
    13381 ttgccctctg caacagctcg atcgtattgg tctagaagat gctgcacgcc ttccgggaat
    13441 cgtgcagcgc cggccagcac ttctcgcatg ccctcctcga tacgtttggc gatggccacc
    13501 tcaccctcac gtgtcaggag c
//
LOCUS       DBNHLM010000102         4484 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_15007, whole genome shotgun sequence.
ACCESSION   DBNHLM010000102 DBNHLM010000000
VERSION     DBNHLM010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_15007"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2397
                     /locus_tag="AC16QF_04745"
     CDS             <1..2397
                     /locus_tag="AC16QF_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6447893.1"
                     /translation="IDTPVAVSVMSEEDIERYNTRDLAQLTQRIPGLSIVNGQGGGAG
                     GNITIRGIGKPPGTSDYGIDAPVSLVIDGMAFSRNHMIKTGFFDSEAVEVLKGPQALY
                     FGKNSPAGVIAIRSKSPEVGGEMELNVRAAYEFVTEDPSIEAGISLPIGEHLAVRFAA
                     RGQDMDGGWLKNTAQPLDVTGLYWEQDGEPFFTGGPSYKEFPAQSQTVWRMTTVWEPT
                     DTFDATLKLFRSYTKRNDAGQVVQWMCADGEGANPAYLGFFPDPYQVCPDRKVKLKGT
                     GSLPPPAIADAAPFIDRDSRFHDKLDQYMHTLQLNWEVGNFTLSSVTGYWDYRHREYT
                     NYDYTTAAVVVSKQGESGEAFTQELRLASSFDGPINFTLGAFYENAERELNAPVQILP
                     SVLFVDADNPDERPLVPYQAGSNPANSNDAAYDGTYINYHQIWNNDIDSWSVFASVDW
                     EVTDKLEISGGLRYTEEDRKATGGNVFENSGFLGFGPGDVVYSPKDKSDNVSPEITVS
                     YHINDDMMVYGSFKTGFQSAGISNPGTVPNLSSLPVDVQNDTLIFDETTIEGFEAGLK
                     GTFFEGRMRADLAAFWYESKDLQVGIFNSNTTTFTLQNAAVAHNWGFEGNMIYQANDR
                     LQFRLAGQFNHLEFDEWEDAGCHPVDGALGDDLPTQGPGCHIGPDGVAIQDLSGVRYG
                     GPPFQANVGFTYDMPLMRDWRLTIDWDTIHHNKGKRTLNQPFTEIPSRTVTNIGATLS
                     QDSGNWEAGVICTNCFNDLYVYAISNRPLAKIIPGERGDLYGQVAPPRLVSLYLTYRM
                     "
     gene            complement(2461..3786)
                     /locus_tag="AC16QF_04750"
     CDS             complement(2461..3786)
                     /locus_tag="AC16QF_04750"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6447894.1"
                     /translation="MAEARVTTNSTQPPRTPITAERYISREYMAREWEKIWSKMWLVA
                     GVEQDVAEPGDYFVFNLEPESIIVSRTSDGELAAFYNVCQHRGAKVVLSDLGALEQYT
                     CPYHGWTYSNDGKLVHVPDEDRFSQGVPKAELSLKPLRVEAWAGVIWVCMDPDAPTLD
                     EFLGPIKAMVEPFRTREMRLIEDQSVRLACNWKAVFDNFGELYHVEHIHPQHETIFDC
                     PASTAELFDKGHTRVLIDGFTVNTRLPIPDEVPMTQWAQMEALGMDKDDYKGRVLDVR
                     RDIQIKKREMAPVLGYNYDTLTDEQLSDIVQYNVFPNAILVIQPEELWILRSRPRGSD
                     PDKCYWDKLALRMFPDRQAQKRANISFNLDQEGVPTGNARPEHDEFEQEDIIAGRKTM
                     TITLDQDIHLIRDVQKGMHSRGFGEAWLNDDESRVQHFHAWLDRYMAGG"
     gene            complement(3861..>4484)
                     /locus_tag="AC16QF_04755"
     CDS             complement(3861..>4484)
                     /locus_tag="AC16QF_04755"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6447895.1"
                     /translation="GGEATFVAADMASDADIQRMVNAAVEAYGSLDYAFNNAGWFGAE
                     PALHEYDDAQWAEVIGVNLTGVFRCMKHQIAAMLACNERQPAHRAIVNNASTVGHRGS
                     DRSGPAYTTAKHGVIGLTRQAALTYVNQGVRVNAVSPGPSVSAVSEHLQQQQAEVVQA
                     MLASLNPTGQLVPAEEIARTVAFLCSDQALMINGHAIPLDGGQLAKL"
BASE COUNT          788 a         1418 c         1430 g          848 t
ORIGIN      
        1 atcgacaccc ctgtcgccgt gtcggtgatg agcgaggaag acattgagcg ttacaacacc
       61 cgcgaccttg cccagctcac ccagcgcatt ccgggtcttt ccatcgtcaa cggccagggt
      121 ggcggcgccg gtggcaacat caccatccgc ggtattggta aaccccccgg aacgagtgac
      181 tatggcatcg acgcacccgt gtcactggtc atcgatggga tggcgttttc ccgcaaccac
      241 atgatcaaga ccggcttctt cgacagcgaa gcggttgagg ttctcaaagg tccccaggca
      301 ctctacttcg gcaagaacag ccccgccggc gtgatcgcca ttcgcagcaa gagccccgag
      361 gtgggcggcg agatggagct gaacgtccgg gccgcctacg agttcgtcac ggaggatcca
      421 tccatcgagg cgggcatcag cttgcccatc ggtgagcacc tggcggtccg gttcgcggcc
      481 cgtgggcagg acatggacgg cggctggctg aagaacactg cccagccgct ggatgtcacg
      541 ggcctgtatt gggagcagga cggcgagcct ttcttcacgg gcgggccttc ctacaaggag
      601 tttcctgctc agagccagac ggtctggcgc atgaccaccg tctgggagcc gacggacacg
      661 ttcgatgcca ccctgaagct gttccggtct tacaccaaac gcaacgacgc ggggcaggtc
      721 gttcagtgga tgtgcgccga cggtgaaggc gcgaacccgg cctatctcgg cttcttccca
      781 gatccgtacc aggtctgtcc ggaccggaag gtcaagctga agggcacggg ctccctgccg
      841 ccgccggcca tcgccgacgc agcacctttc atcgaccggg actcccgctt ccacgacaag
      901 ctcgatcagt acatgcacac ccttcagctc aactgggagg tgggcaattt cacgttgtct
      961 tcggtgaccg gttactggga ttaccgccat cgggagtaca ccaattacga ctacaccact
     1021 gccgcagtgg tggtgtctaa gcagggcgag tccggcgagg ccttcaccca ggaactgcgg
     1081 ctggcctcca gcttcgacgg acccatcaac tttacgttgg gtgccttcta cgagaacgcc
     1141 gagcgtgagc tgaacgcccc ggtgcagatc ttgccctccg tgttgttcgt tgacgcggat
     1201 aatcctgatg aaaggccgtt ggttccctat caggcgggat caaatccggc aaactccaac
     1261 gatgcggcct atgacggcac gtacatcaac taccaccaga tctggaacaa cgacatcgac
     1321 agctggtcgg tgttcgccag cgtggactgg gaggtcaccg acaagctgga gatctccggc
     1381 ggcctgcgtt atacggagga ggatcgcaag gcgacgggcg gcaacgtctt tgagaacagc
     1441 ggcttcctcg gattcggtcc tggggacgtc gtctacagcc cgaaggacaa gtcggacaac
     1501 gtttcccccg agatcaccgt cagctatcac atcaacgacg acatgatggt gtacgggtcg
     1561 ttcaagaccg gcttccagtc ggctggtatt tccaatccgg gcacggtgcc caacctgtcc
     1621 agcctgccgg tcgacgtgca gaacgacacg ctgattttcg acgagaccac catcgagggc
     1681 ttcgaggcgg gactgaaggg caccttcttc gaaggccgca tgcgggccga cctggcggcg
     1741 ttctggtacg agtcaaagga cctgcaggtg ggcatcttca actccaacac cacgacgttc
     1801 accctgcaga acgcggccgt ggcccacaac tggggcttcg agggcaacat gatctaccag
     1861 gctaacgacc ggctgcagtt ccgcctggcc ggacagttca accacctgga gttcgacgag
     1921 tgggaagatg cgggatgtca tcccgtggac ggtgcgcttg gcgatgacct gcccacccaa
     1981 ggcccgggat gccacatcgg tccggacggc gtggcgattc aggacctctc cggcgtccgt
     2041 tacggtggcc cgccgttcca ggccaacgtc ggcttcacgt acgacatgcc gctgatgcgt
     2101 gattggcgcc tgaccatcga ctgggacacc attcaccaca acaagggcaa gcgtacgctg
     2161 aaccaaccct tcacggagat cccgagccgt acggtgacca acatcggagc cacgttgagt
     2221 caggatagcg ggaattggga agcgggcgtg atctgcacga actgcttcaa cgatctgtac
     2281 gtttacgcca tttccaaccg cccgctggcg aagatcattc ccggcgagcg cggcgacctc
     2341 tacggccagg tggcgccgcc gcgtctggtg agcctgtacc tgacctatcg catgtagcga
     2401 caggtttgcg actgaaaagc gggccgaatg gcccgctttt ttttttgcct cgttctggcc
     2461 tcaacccccc gccatgtagc ggtcgagcca cgcgtggaag tgctgcacgc ggctctcgtc
     2521 gtcgttcagc caagcttcgc cgaagccgcg ggagtgcatg cctttctgga cgtcgcggat
     2581 caggtggatg tcctgatcca gggtgatggt catggtcttg cgcccggcga tgatgtcctc
     2641 ctgctcgaac tcgtcgtgct cggggcgcgc attgcccgtg ggcacccctt cctggtccag
     2701 gttgaacgag atgttggccc gcttctgcgc ctggcggtcc ggaaacatgc gcagggccag
     2761 cttgtcccag tagcacttgt cgggatcgct gccccgcggg cgcgagcgca ggatccacag
     2821 ctcctcgggc tggataacca ggatggcgtt gggaaagacg ttgtactgca cgatgtcgga
     2881 gagctgctcg tcggtgaggg tgtcgtagtt gtagcccagc accggggcca tctcgcgttt
     2941 cttgatctgg atgtcgcgcc tgacgtccag cacccggccc ttgtagtcgt ccttgtccat
     3001 gcccagggcc tccatctggg cccactgggt catgggcacc tcgtcgggga tgggcaggcg
     3061 ggtgttgacc gtgaagccgt cgatgagcac ccgcgtatgg cccttatcga acagctcggc
     3121 cgtggaggcc gggcagtcga agatggtctc gtgctggggg tgaatgtgct ccacgtggta
     3181 gagctcgccg aagttgtcga acacggcctt ccagttgcac gccaggcgca ccgactggtc
     3241 ctctatcagg cgcatctccc gggtgcggaa cggctccacc atggccttga tggggccgag
     3301 gaactcgtcc agcgtgggcg cgtccgggtc catgcagacc cagatgacgc cggcccaggc
     3361 ttccaccctg agcggcttca gcgaaagctc ggccttggga accccctgac tgaagcggtc
     3421 ctcgtccggc acgtggacga gctttccgtc gttgctgtac gtccagccgt ggtaggggca
     3481 cgtgtactgc tccagcgcgc cgagatccga gagcaccacc ttcgcaccgc ggtgctggca
     3541 gacgttgtaa aaggcggcga gctcaccgtc gctggtccgg gagacgatga tggactcggg
     3601 ctcgaggttg aagacgaagt aatctccggg ctcggccacg tcctgctcca cgccggccac
     3661 cagccacatc ttcgaccaga tcttctccca ctcccgggcc atgtactcgc gcgagatgta
     3721 gcgctcggcg gtgatgggcg tacgtggcgg ctgcgtgctg ttggtggtaa ctcttgcctc
     3781 tgccatggcg ttgctccctt tgtttccctg cggcctgtgt aacaccttgg gagagtgtcc
     3841 gcaacgccgg gcggcggcaa tcagagcttg gccagctgcc cgccgtccag ggggatggcg
     3901 tgcccgttga tcatcagcgc ctgatccgag cagagaaacg ccacggtccg ggcgatctcc
     3961 tcggcgggca ccagctgccc ggtggggttc agagaggcaa gcatggcttg taccacctcg
     4021 gcctgctgct gctgcaggtg ctcgctcaca gcgctcacgc tgggccccgg cgagacggcg
     4081 ttcacccgga cgccctgatt cacgtacgtc agggccgcct ggcgggtcag cccgatgacg
     4141 ccgtgcttgg cggtggtgta ggcgggcccg gagcggtcac tgccgcggtg accgacggtg
     4201 gaggcgttgt tgacgatggc tcggtgcgcc ggctgcctct cgttgcacgc cagcatggcc
     4261 gcgatctggt gcttcatgca gcggaacacg ccggtgagat tcacgccgat gacctcggcc
     4321 cattgggcgt cgtcgtactc gtgcagcgct ggctcggcgc cgaaccagcc ggcgttgttg
     4381 aaggcgtaat cgaggctgcc gtaggcctcc accgcggcat tcaccatgcg ttggatgtcc
     4441 gcgtcggaag ccatgtccgc ggcgacgaac gtggcttcac cgcc
//
LOCUS       DBNHLM010000103        10619 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1032715, whole genome shotgun sequence.
ACCESSION   DBNHLM010000103 DBNHLM010000000
VERSION     DBNHLM010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10619)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10619)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10619
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1032715"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1687
                     /locus_tag="AC16QF_04760"
     CDS             <1..1687
                     /locus_tag="AC16QF_04760"
                     /inference="COORDINATES: protein motif:HMM:NF016251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HIR6447896.1"
                     /translation="SPSSPEALLQQADLANGREAARLYLSATESFIQANRLDEAARSF
                     QRIDQALLSGARTADYRYAAAEIALHAGEPDRAAALLGAGEPGGGSARRRGLWAATLP
                     GRICHAAGDDQCAIRELTGLEVPGDQRQELHDVIWRYLGFAAGFSAPAQAGRTSGREQ
                     GWWALKATVLQGISVEDQRQRVDDWRGAWPDHPAASVLPTALRAFDRDAPKVRRVGLL
                     LPLSGPLAVAGRAVRDGYIAAYLHESAQSEQEIIVYDTAAAPLPSLYEKALLDEVDVL
                     IGPLTKEEASALSGLAPSVPTLVLNYLEDGQRAPSNVYQLGLAIEDEADTMVSRLLED
                     GAERLLVVHNDQDWSRRAARRLTSSWPYPVTREFFPDVKTITESVGRAMQIDASLERR
                     DELASLLGTDLEFLPRARGDLDAVVALVDTVEANALVPALRFHFAQDLPVYASSQTVR
                     GATAQELGELAGFRVSELPWILFSDPIYLAMADVFLLQGNRFAALYALGVDAYRVSGR
                     LTLLASGGVHQLLGSTGLLTVDGSGRFRRELAWGLIRRGRLEPLPVMAGQSAL"
     gene            1684..2049
                     /locus_tag="AC16QF_04765"
     CDS             1684..2049
                     /locus_tag="AC16QF_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR6447897.1"
                     /translation="MTPQARAGAGAESAALDHLRARGLELVARNFRCRLGELDIVMVD
                     GNELVFVEVRLRNHARFGDGAASITAAKRQKLARAARAFLQRHPRYQSCPCRFDVVAI
                     KGEGQRMEMRWLKNAFAVG"
     gene            complement(2102..2494)
                     /locus_tag="AC16QF_04770"
     CDS             complement(2102..2494)
                     /locus_tag="AC16QF_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HIR6447898.1"
                     /translation="MKAKAKRPYLLRALYDWLVDSSLTPYVLVSAEDEAAMVPADYVN
                     DGKIVLNISPSAVRGLVIDDAGLSFDGRFGGAPYPVSAPISSVLAIYAKETGEGMIFD
                     PEEFPSRTDDSQEAPKDRPSGPELKVIK"
     gene            complement(2513..3145)
                     /locus_tag="AC16QF_04775"
     CDS             complement(2513..3145)
                     /locus_tag="AC16QF_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6447899.1"
                     /translation="MSVVTKRSSMTFFSDPRDHYCHRVRMVLAEKGVTVDIVDVDPSR
                     KPEDLAEINPYNTLPTLLDRDLVLYEANVIMEYLDERFPHPPLLPVYPVQRALSRLWI
                     TRVEKEWSGRLDILMAGKGRETTITKARKELRESIISIAPIFSEKPFFMNDEFTLVDC
                     CVAPILWRLKYVGINLPERSTRPLQRYMQVMFERSSFRESLSEAELEMRD"
     gene            complement(3200..3922)
                     /locus_tag="AC16QF_04780"
     CDS             complement(3200..3922)
                     /locus_tag="AC16QF_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003094157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HIR6447900.1"
                     /translation="MKSRSSFLWALTAATLTFSAGAFPAGSGWPLDSIDPDLEDLPSL
                     QNGFKLYVNYCIGCHSVKYQRYERTADDLGIPHDIAMDNLVLTGQKIGELMTTSMDPE
                     LAKNWFGAPPPDLTMVARVRGTDWLYTYLRTFYQDEARPFGVNNLVFENVGMPNVLAG
                     LQGEQVLDDSGSPELVAGTGQLSPEEFDQVIYDIVNFLYYTGDPSRLERHRTGIYVLL
                     FLVVLYVFTYLLGREYHKEIVD"
     gene            complement(3926..5230)
                     /locus_tag="AC16QF_04785"
     CDS             complement(3926..5230)
                     /locus_tag="AC16QF_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017132656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR6447901.1"
                     /translation="MEQSQPGRIARGWSNFMEWVDERFPLTETFEYHMSRYYAPKNFN
                     VWYLFGVLSMVVLVIQLLTGIWLTMSYVPSGDGAFASVEYIMRDVEYGWLLRYLHTTG
                     ASAFFLVVYLHMFRAMMYGSYRKPRELLWLIGMAIFACLIVEGFLGYLLPWGNMSYWA
                     AQVIISLVGAIPVVGPDLMEWVRGDFLVSDITLNRFFALHVVALPLVLVMLVFVHIVA
                     LHHVGSNNPDGIEIKQNKDENGIPLDGVPFHPHYTIGHDLPAIVLFLFFFCIVVFYVP
                     EMFGYFIEKPNFEEANSLKTPEHIAPVWYYTPFYAMLRAATFPLFGIPAKFWGFVVMA
                     GAIVLPAVLPWLDRSPVKSIRYKGWLSKGMLALFVISFFILGYLGTIAPSKPATALAQ
                     ICTVVYFAYFVLMPWYTRVEKTKPVPDRVTTKATRAAKAEGA"
     gene            complement(5230..5880)
                     /gene="petA"
                     /locus_tag="AC16QF_04790"
     CDS             complement(5230..5880)
                     /gene="petA"
                     /locus_tag="AC16QF_04790"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HIR6447902.1"
                     /translation="MSADKAATDSAAVPKSLDPSKVNKERRYFLIGASAVVGGIGIVG
                     AAVPFVASWQPSAKARALGAPVTVDISKLRPGEILGPTPAWRGNPVFVVYRDEDALRR
                     LEQENDRLADPDSDNAEQQPDYAKNPWRSKRPEIGVYLGLCTHLGCSPKYHGEVKPEP
                     FAADWEGGFFCPCHGSLFDLAGRVVKGVPAPDNLPVPPYQFVSDTVVVIGVDEETA"
     gene            complement(6011..6403)
                     /gene="rpsI"
                     /locus_tag="AC16QF_04795"
     CDS             complement(6011..6403)
                     /gene="rpsI"
                     /locus_tag="AC16QF_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR6447903.1"
                     /translation="MSANQFYGTGRRKTSAARVFISSGTGNIFVNNQSLDSYFGRETS
                     RMIVRQALFVADVADKVDIRVTVRGGGNSGQAGAIRHGIARALVAHDESLRGALRKAG
                     FLTRDAREVERKKVGLRKARKRPQFSKR"
     gene            complement(6438..6866)
                     /gene="rplM"
                     /locus_tag="AC16QF_04800"
     CDS             complement(6438..6866)
                     /gene="rplM"
                     /locus_tag="AC16QF_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010797309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR6447904.1"
                     /translation="MKTVSARAQDVQRSWLLVDAEDKTLGRLATEVARRLRGKHKPEY
                     TPHVDTGDYVVVINADKVRVTGNKTTDKIYYRHSGYPGGIKGISFEHLQQKHPERIIE
                     KAVKGMLPRNTLGRAMFRKLKVYAGPEHPHSAQQPEPINL"
     gene            complement(6949..8049)
                     /gene="zapE"
                     /locus_tag="AC16QF_04805"
     CDS             complement(6949..8049)
                     /gene="zapE"
                     /locus_tag="AC16QF_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HIR6447905.1"
                     /translation="MSPLQKYEEQLTRSGFFDDPAQREVAGLLDDLYRAFMARERLHA
                     SRLRRALRVLGIGAEPEPVRGLYLWGTVGRGKTLLMDLFYECLPGDVRLRMHFHRFMQ
                     RVHHQLTSLAGTPNPLQQVAERFASEASVLCFDEFAVSDIGDAMILAELLAELFRRGV
                     TLVATSNVEPDNLYENGLQRRRFLPAIELIRQHTVIHQVGGDVDYRLRVLERAEIYHV
                     PLDAGAERSLARSFDALAPEAPRDDVDLEIEGRMIRARRVAEDVAWFNFSDLCEGPRS
                     QNDYIELSRIFHAVLISGVPRFNTRSEDAARRFISLVDEFYDHGVKLIVSAEAEPASL
                     YGGERLRFEFERTVSRLLEMQSHDYLARSHRA"
     gene            8160..9212
                     /locus_tag="AC16QF_04810"
     CDS             8160..9212
                     /locus_tag="AC16QF_04810"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003115246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR6447906.1"
                     /translation="MRELVRYILVPALVGGAVGLGVMLTSQVNGTPTKPGYAAAVKRA
                     APGVVNIYSSKLVQHPMCEMPRFRDWCERFAGADRGQMQNSLGSGVIVREDGYILTNN
                     HVIEGADEIIVAFSDGQATTATLVASDPETDLAVIRVQASGLTPVPRDPSSVVEVGDV
                     ALAIGNPFGIGQTVSSGIISAKGRAGISRSPYEDFIQTDAAINPGNSGGALIDANGNL
                     IGINTLIFSRSGGSEGIGFAIPAQLAMAVLEEIIETGRVVRGYLGVELSSTPPAGAGV
                     GLEVTRLMSPSPAASAGLRPGDLILSINEQPAVSAAGVTRQIAHVEPGSELEIEVLRG
                     NRQISLTARAGERPQP"
     gene            complement(9230..10558)
                     /gene="hisD"
                     /locus_tag="AC16QF_04815"
     CDS             complement(9230..10558)
                     /gene="hisD"
                     /locus_tag="AC16QF_04815"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR6447907.1"
                     /translation="MTEPALEIARLATGDADFPDRLARLLYWDTGLEGEVTRAAAEVI
                     EAVRAGGDDALLALTARYDRYECPSAAALEIAASELEAAYDALGSADREALNLAAQRI
                     REYHNHQLAGDWEYTDSDGNLLGQRVMALERVGLYVPGGQAAYPSTVLMTAVPARVAG
                     VDEIIMTVPTPDGVRSPLVLAAAHVAGVDRVFTVGGAQAIAALAYGTRTIPKVDKIVG
                     PGGAYVAAAKRLVFGTVGIDVIAGPSEVLVIADDTAPPEWLALDLFSQAEHDASAQAI
                     LVSPDPDVLDAVHDAMASLIGERSRATTIRASLAARGALIRAADLADAVALANRIAPE
                     HLELAVADPDALLPDVRNAGAIFIGAHSPEVMGDYVAGPSHVLPTFGTARFSSPLGVY
                     DFVKRSSVIRLSPGGSEKLARASATLARGEGLEAHARAAELRITGLDHNR"
BASE COUNT         1889 a         3475 c         3416 g         1839 t
ORIGIN      
        1 ctccccctca tctcccgaag ctctcctgca acaggcggat ctggccaacg ggcgggaagc
       61 agcccggctg tacctgtcgg ccaccgagag cttcattcag gccaaccggc tcgacgaggc
      121 tgcccgatct ttccagagga tcgaccaggc gttgctctcc ggcgcccgga cggccgacta
      181 tcgatatgcc gccgccgaga tcgcgctgca cgccggcgag ccggatcggg cggccgcgct
      241 gcttggcgcc ggggagccgg gcggcggctc ggcgcgacgg cgcggtctgt gggcggccac
      301 cctgcccggc cgcatctgcc acgccgccgg cgatgaccaa tgcgcgatcc gggagctgac
      361 cggactcgag gtacccggcg accagcgcca ggagctgcac gacgtcatct ggcgctatct
      421 gggttttgca gccggtttct ccgcgcccgc tcaggccggc cgcacctccg ggcgcgagca
      481 gggttggtgg gctctcaagg ccacggtgct gcagggcatc tccgtggaag accagcgtca
      541 gcgtgtggac gattggcgcg gcgcctggcc cgatcacccg gcagcttccg tcttgcccac
      601 ggctctccgc gccttcgacc gggacgcccc gaaggtgcga cgggtcgggt tgctgctgcc
      661 gctcagcggc cccctcgccg ttgccgggcg cgccgttcgg gacggctaca tcgccgccta
      721 tctgcacgag tctgcccaga gcgagcagga gatcatcgtc tacgacacgg ccgccgcacc
      781 gctgccgagc ttgtacgaga aggccctcct ggacgaggtg gacgtgctca tagggcccct
      841 gacgaaggaa gaggcgtccg ccctcagcgg gctcgccccg tccgttccca ccctggtgct
      901 caactatctc gaagacggcc agagggcgcc ctcgaacgtc taccagctcg ggcttgccat
      961 cgaggacgag gccgacacca tggtgagcag gctcctggag gatggcgcag agcgcttgct
     1021 ggtcgtccac aacgatcagg attggtcgcg tcgagcggcg cggcgactta cttccagctg
     1081 gccctatccg gtcacgcgcg agttctttcc ggacgtcaaa accatcaccg aatccgtcgg
     1141 acgggccatg cagatcgacg cgagcctcga gcgccgcgac gagctggcca gtctcctggg
     1201 gaccgacctg gagtttctgc cacgcgccag gggggacctc gacgccgtgg tcgcgctcgt
     1261 cgataccgtc gaggccaatg ccctcgtgcc tgcgttgcgc tttcattttg cccaggatct
     1321 gcccgtgtac gcgagctcgc agacggtgcg tggagcaacg gcacaggaac tcggagagct
     1381 tgccgggttt cgcgtcagcg agctgccctg gatattgttc tccgacccca tctacctggc
     1441 catggccgac gtgttcctcc tgcagggcaa tcgcttcgcc gccttgtacg cgcttggcgt
     1501 ggacgcttat cgcgtgagcg ggcgcctgac gctgctggcc tccggcgggg tccaccagct
     1561 gctgggcagc acgggcctgc tcacggtgga tggctccgga cggttccgtc gggagctggc
     1621 gtggggactg atccgcaggg gccgcctcga accgctcccg gtgatggccg ggcaatcggc
     1681 cctctgacgc cccaggcacg ggcaggtgcc ggggcagaat ccgccgcgct ggaccatctc
     1741 cgcgcgcggg gcctcgagct ggtagcccgc aatttccgct gccgtctggg cgagctggac
     1801 atcgtcatgg tggacggcaa cgagctggtt ttcgtcgaag tccgtctgcg caaccacgct
     1861 cgcttcggcg acggcgcggc gagcatcacc gccgcaaagc ggcagaagct ggcaagggcc
     1921 gcccgggcat ttctccaacg ccacccccga taccagagct gcccttgccg gttcgacgtg
     1981 gtggccatca aaggagaggg tcaacgcatg gagatgcgat ggctaaaaaa cgcgttcgcg
     2041 gtcgggtaat gattcgatcc tgccgccagc tggcgcggtg aagcaatgag aaatgcagcc
     2101 gctacttgat gaccttcagc tcggggccgg acgggcggtc cttcggcgct tcctgggaat
     2161 catcggtacg ggaaggaaac tcctccgggt cgaagatcat gccctcgccg gtctccttgg
     2221 cgtagatggc cagaacactc gagatgggcg cggataccgg atagggcgct ccaccgaacc
     2281 gcccgtcgaa gctgagcccg gcgtcatcga tgacaagtcc tcgcacggcc gacggcgata
     2341 tgttgagaac gatcttgccg tcgttgacgt agtcggccgg caccatcgcc gcttcgtcct
     2401 ccgcggagac gagcacgtac ggggtgagcg aagagtcgac gagccagtcg taaagggctc
     2461 gaagcaggta aggacgtttc gccttggctt tcacgccaga ccgcccccgg ggtcagtccc
     2521 gcatctcgag ctcggcctcg gagaggcttt ctcggaacga gctgcgctcg aacatgacct
     2581 gcatgtagcg ctgcagggga cgcgtcgagc gttccggcag gttgatgccg acgtacttca
     2641 atcgccacag gatgggcgcc acgcagcagt cgacaagcgt gaactcgtcg ttcatgaaaa
     2701 aaggcttctc gctgaaaatc ggcgcgatgc tgatgatgct ttcgcgcagt tccttgcgcg
     2761 ccttggtgat ggtcgtctcg cgacctttgc cggccatcag aatatccagc cggccgctcc
     2821 actccttctc cacacgcgtg atccaaagcc gcgacagagc gcgctggacg gggtagacgg
     2881 gcagaagcgg cgggtgcgga aaccgctcgt ccagatattc catgatcacg ttggcctcgt
     2941 agagcacgag gtcgcggtcg agcagcgtcg gcaaggtgtt gtacggattg atctcggcca
     3001 ggtcctccgg cttgcgcgac ggatccacgt ccacgatatc cacggtcacg cctttctcgg
     3061 cgagcaccat ccgcacccgg tggcagtagt ggtcccgggg gtcggagaag aacgtcattg
     3121 aggaccgctt tgtgactacg ctcatgattt acctttcgtc tgaaattcgc gttacaagaa
     3181 cctgccggcc cccaacgatc tagtcgacga tttccttgtg atattcgcgg ccgaggagat
     3241 acgtgaacac gtaaagcact accagaaaca gcagcacgta aattcccgta cggtgacgtt
     3301 ccagacggct cggatccccc gtgtagtaga ggaagttcac gatgtcgtag atcacctgat
     3361 cgaactcctc gggcgagagt tgcccggtgc ctgcaaccag ctcgggtgaa ccggaatcat
     3421 ccagcacctg ctctccctgg aggccggcca gaacgttggg catacccacg ttctcgaaca
     3481 ccaggttgtt gactccgaag ggccgcgcct cgtcctggta gaacgttcgc aggtacgtgt
     3541 acagccagtc cgtgccgcgc acccgcgcga ccatggtcag atccggggga ggcgcgccga
     3601 accagttctt ggcgagctcc ggatccatgc tggtggtcat cagctctccg atcttctggc
     3661 cggtcaggac caggttgtcc atcgcgatgt cgtgaggaat ccccagatca tccgccgtcc
     3721 gctcgtagcg ctgatacttc accgagtggc agccaataca gtagttgacg taaagcttga
     3781 agccgttctg cagggaaggc aggtcctcca gatcaggatc gatgctgtcc agcggccagc
     3841 ccgagccggc aggaaacgcg cctgcagaga atgtcagcgt cgcggcggtc agggcccaga
     3901 gaaacgagct gcgtgatttc atgccttacg ccccctcggc ctttgccgct cgcgtcgcct
     3961 tggtagtcac cctgtccggc accggtttcg tcttctccac ccgcgtgtac cagggcatga
     4021 gcacgaagta cgcgaagtag acgacggtgc agatctgggc gagagccgtt gctggcttcg
     4081 acggcgcgat ggttcccagg tatccgagta tgaagaagct gatcacgaac agcgccagca
     4141 tccccttgga cagccacccc ttgtagcgga tggatttcac cgggctgcgg tcgagccacg
     4201 gaaggactgc gggcagcacg atggcgccgg ccatgactac gaatccccag aatttggcgg
     4261 gaatcccgaa gagagggaac gtggccgcgc gcagcatcgc gtagaacggc gtgtagtacc
     4321 acaccggcgc gatgtgctcg ggtgtcttca gcgagttggc ctcctcgaag ttcggctttt
     4381 cgatgaagta gccgaacatc tctggcacgt agaaaaccac gatgcagaag aagaagagaa
     4441 agagcacgat ggccggaaga tcgtgtccga tggtgtagtg cggatggaag ggaaccccgt
     4501 ccaggggaat cccgttctcg tctttgttct gcttgatttc gataccgtcg gggttgttgg
     4561 agccgacgtg atgcagagcg acgatgtgca cgaagacgag catcaccagc acgagcggca
     4621 gggccaccac gtgaagggcg aaaaagcgat tcagcgtgat gtccgagacg agaaagtcgc
     4681 cgcggaccca ctccatcaga tcaggcccga ccacgggaat cgcgccgacc agcgagatga
     4741 tcacctgggc ggcccagtag gacatgttgc cccagggcag caggtagccg agaaaccctt
     4801 ccacgatcag gcaggcaaat atcgccatgc cgatgagcca gagcagttcc cgcggcttgc
     4861 gataggagcc gtacatcatg gcccggaaca tgtgcaggta aacgaccagg aagaaggccg
     4921 aggccccggt ggtgtgcaga tagcgcagga gccagccgta ctcgacgtcg cgcatgatgt
     4981 actcgacgga agcgaaagcg ccgtcgccgc tgggcacgta gctcatggtc agccagatgc
     5041 cggtcagcag ctggatcacc agaacgacca tcgagagaac gccgaacaga taccagacat
     5101 tgaagttctt cggcgcgtag taccgggaca tgtggtactc gaaggtctcg gtgagcggaa
     5161 acctctcgtc gacccattcc atgaagttgg accagccgcg cgcaatcctt cccggttgcg
     5221 attgctccat cacgctgtct cctcgtccac gccgatcacc accaccgtgt cgctcacgaa
     5281 ctgatacggg ggcactggca ggttgtccgg cgccgggacc cctttgacga cccgtcccgc
     5341 cagatcgaaa agggatccgt gacacggaca gaagaagccg ccttcccagt cagcggcaaa
     5401 gggttccggc tttacctcac catggtattt cggcgagcaa cccaagtgcg tgcacagccc
     5461 caggtagacg ccgatctccg gacgcttcga ccgccacggg ttctttgcgt aatctggctg
     5521 ctgctcggcg ttgtcggaat ccgggtcggc gagccggtcg ttctcctgct cgagcctgcg
     5581 caaggcgtcc tcgtcgcggt agaccacgaa caccgggtta ccccgccatg ccggggtggg
     5641 tcccagtatt tcaccgggcc gaagcttgct gatatccacg gtcaccggag caccgagcgc
     5701 acgcgctttc gcgctgggtt gccacgacgc cacgaagggc accgctgcgc ccacgatgcc
     5761 tataccacct accacggcgg aggctccgat cagaaagtag cggcgttcct tgttgacctt
     5821 ggaagggtcg agactcttag gaacggcggc agaatccgtt gcagctttat cagcgctcaa
     5881 gggtcgctcc cagcatattc aaacagcccc gcattctagc acacgcgggt gcacatcctt
     5941 gcccgcagga ggctcgcgca gcccgcgcgg aggtccttgt ggacggggcc cgaacggcgc
     6001 gccgacgcct ctatcgcttg gagaactgcg ggcgtttacg cgccttgcgc agaccgacct
     6061 tcttgcgctc cacctcgcgc gcgtcgcggg tcaggaaccc ggccttgcgc aacgcacctc
     6121 gaaggctttc gtcgtgagca acgagcgccc gggcgatgcc gtgcctgatc gcgccagcct
     6181 ggcccgagtt gcctccaccg cgaacggtca cgcgaatgtc caccttgtct gcgacgtcgg
     6241 cgacaaagag cgcctggcga acgatcatgc gggacgtctc gcgcccgaaa tagctgtcga
     6301 ggctctgatt gttgacgaag atgttgccgg tgcccgaaga gatgaaaacg cgggctgcgg
     6361 aagtcttgcg tcgccccgtt ccgtaaaatt ggtttgctga catgctgtgt gcgtcctggg
     6421 cctgatcggc taactggcta taggttgatc ggttcgggtt gctgggcgct gtgcggatgt
     6481 tccgggccgg cgtagacctt gagcttgcgg aacatggccc ggccgagcgt gttgcgaggc
     6541 agcatcccct tgacggcttt ctcgatgatg cgctccgggt gcttctgctg cagatgctcg
     6601 aagctgatcc ccttgatgcc gccgggatag ccgctgtggc ggtagtagat cttgtcggtg
     6661 gttttgttcc ccgtcacccg caccttgtcg gcgttgatga ccacgacgta atcccccgtg
     6721 tccacgtgag gagtgtattc cggcttgtgc tttccccgga gacggcgagc cacctcggtt
     6781 gccaggcgac caagcgtctt atcttctgcg tccaccaata gccacgaacg ctgcacgtcc
     6841 tgcgcccgcg cgcttaccgt cttcatgagc ccttcaagat gccgggattt tgggagcgcg
     6901 aatgttagcg gagggcctga ccctttacaa ccagcccgac gggccttctc aggccctgtg
     6961 agagcgggcc agatagtcgt gggactgcat ctccagcagc cggctcaccg tgcgctcgaa
     7021 ctcgaaccgc aggcgctctc ccccatagag cgaggcgggc tcggcctcag cggagacgat
     7081 cagcttgacg ccgtgatcat agaactcgtc caccaggctg atgaaacgcc tcgcggcgtc
     7141 ttccgatcgg gtgttgaagc gaggcacccc gctgatcagc acggcgtgga agatccgcga
     7201 gagctcgatg tagtcgttct ggctgcgggg accttcacag agatcggaaa agttgaacca
     7261 ggccacgtcc tcggcaaccc gccgcgccct gatcatccga ccttcgatct cgagatccac
     7321 gtcgtctcgg ggcgcttccg gcgccagggc atcgaagctc cgggcaaggc tccgctccgc
     7381 cccggcgtcc agcggaacgt gatagatctc cgcccgctcc agaaccctca agcgataatc
     7441 cacgtctccc ccgacctgat ggatcacggt gtgctgcctg atgagctcga tggcgggcag
     7501 aaaacgacgg cgctgcaggc cgttctcgta aaggttgtcg ggctccacgt tggacgtcgc
     7561 caccagcgtc acgccacgcc tgaacagctc ggccagcagt tccgccagaa tcatggcgtc
     7621 gccgatgtcc gaaacggcaa actcgtcgaa gcagaggacg cttgcctcgc tggcaaaccg
     7681 ctctgcaact tgctgcagag ggttcggcgt gcccgcaagg ctcgtcagct gatggtgaac
     7741 gcgctgcatg aaccggtgga agtgcatgcg cagacgaacg tccccgggaa ggcactcgta
     7801 gaagaggtcc atgagcaggg tcttacctcg accgaccgta ccccacagat acaggcccct
     7861 cacaggctcg ggctcagccc cgatgcccag cacgcgcagg gcgcgccgca gccggctggc
     7921 gtgcagcctt tcccgggcca tgaacgcccg gtagagatcg tccagcagcc cggcgacctc
     7981 ccgctgcgcc gggtcgtcaa agaagccgct acgcgtgagc tgttcttcgt atttctgcaa
     8041 cggcgacatc ggcggaactt tacttgctcg gtcaggaacg gcgcaatcgg cgccacccga
     8101 cgggcgaggc ttgccgccgc ggggcgcggc aggagttttt ctgttaacgt ccgccgccta
     8161 tgcgcgaact ggttcgctac atcctggtcc ctgcactggt cgggggtgcc gtcggtctcg
     8221 gggtcatgct caccagccag gtgaacggaa cgccgaccaa gcccgggtac gcagccgcgg
     8281 taaagcgcgc cgcgcccggc gtcgtcaata tctactcgtc caagctggta cagcatccca
     8341 tgtgcgagat gccccggttt cgagactggt gcgagcgctt cgcgggggcc gatcgcgggc
     8401 agatgcagaa ctcgctggga tccggcgtca tcgtccggga ggacggctac attctcacca
     8461 acaatcatgt gatcgagggg gccgacgaga tcatcgtggc gttctctgac ggtcaggcca
     8521 ccacggcgac cctggtcgcc agcgaccccg agaccgatct cgccgtcatc cgcgtgcagg
     8581 cctcgggcct gacgccggtg ccccgtgatc cgagcagcgt cgtcgaggtg ggcgacgtcg
     8641 cgctggccat cggcaacccg ttcggcattg gccagacggt ctcgagcggc atcatcagcg
     8701 caaagggccg ggcgggcatc agccgcagcc cctacgagga cttcatccag accgacgcag
     8761 ccatcaaccc cggaaactcc gggggcgcgc tcatcgatgc caacggcaac ctgatcggca
     8821 taaacacgct gatcttttcc cgctccggcg gctccgaggg catcgggttc gccatccccg
     8881 cacagcttgc catggcggtg ctggaggaga tcatcgagac gggccgcgtg gtccgcggct
     8941 atctcggcgt cgagctgtcc agcacgccgc ctgccggggc aggcgtcggc ctggaagtca
     9001 ccagactgat gtcacccagc cccgccgctt cggccggtct gcgcccgggc gatctgatcc
     9061 tttccatcaa cgagcaaccg gccgtgagcg cggcaggcgt cacgcggcag atcgctcacg
     9121 tcgagccggg cagcgaactg gagatagagg ttctgcgtgg gaatcgccag atctccctca
     9181 cggcgcgcgc cggggagcgc cctcagccct aggagcctca cgggtccggc taacggttgt
     9241 ggtccagccc ggtgatcctc aattcagcag ccctggcgtg ggcttcgagc ccctcgcccc
     9301 gagcgagggt cgccgaggcg cgggcaagct tttcggaccc gcctggactc agccggatca
     9361 cggagctgcg tttgacgaaa tcgtagaccc ccagcggtga cgagaacctg gccgtaccga
     9421 atgtcggcag cacgtggctc ggacccgcca cgtaatcgcc catcacttcc ggagagtgcg
     9481 cgccgatgaa gatcgcgccg gcgttgcgaa cgtcgggcag gagcgcgtcc ggatccgcca
     9541 cggcgagctc gagatgctcg ggcgcgattc ggttggccag cgccacggcg tcggcgaggt
     9601 ccgccgcccg gatcaaggca cctcgcgcgg cgagcgaggc tcggatggtc gttgctctgg
     9661 accgctcgcc gatgagcgag gccatggcgt cgtggacggc atcgagcacg tccgggtccg
     9721 ggctgaccag aatggcctgg gcggaagcat cgtgctccgc ctgggaaaac aggtccaggg
     9781 cgagccactc gggcggcgcc gtgtcgtccg caatcacaag cacctcactg gggccggcaa
     9841 tgacgtcgat accgacggtg ccgaaaacca ggcgcttggc ggcagcgacg tatgccccgc
     9901 cgggaccgac gatcttgtcc accttgggaa tcgtccgcgt gccgtaggcc agcgcggcga
     9961 tggcctgggc gccgccaacg gtaaataccc ggtcgacgcc cgctacatgg gctgcggcga
    10021 gcaccagcgg cgaacgcacg ccatccggcg tggggacggt catgatgatc tcgtccacac
    10081 ccgcgacccg ggcgggcacc gccgtcatca gcaccgttga cgggtacgcc gcctggccgc
    10141 caggcacgta gaggcccacc cgctccagcg ccatcacccg ctggccgagg aggttgccgt
    10201 cactgtcggt atattcccag tctccggcca gctggtggtt gtgatattcg cgaatccgct
    10261 gggcggccag gttcaacgcc tcgcggtctg ctgagcccag ggcgtcgtag gctgcctcga
    10321 gctcggaagc cgcgatctcc agcgccgcgg cgctcgggca ctcgtaacga tcgtacctgg
    10381 cggtgagcgc cagcagggcg tcgtcaccac cggcccgcac cgcttctatg acctcggccg
    10441 ccgctcgcgt cacctcgcct tccaggcccg tgtcccagta aaggagcctg gcgagacgat
    10501 ccgggaagtc cgcatcgccc gtcgcgagcc gggcaatctc gagcgccggt tccgtcatcc
    10561 cgacacgtcc cgcaggctgc aggtgaggct ctgcacggcg tcgaagcggg tcttcatgg
//
LOCUS       DBNHLM010000104        13822 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_494070, whole genome shotgun sequence.
ACCESSION   DBNHLM010000104 DBNHLM010000000
VERSION     DBNHLM010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13822)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13822)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13822
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_494070"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3887
                     /locus_tag="AC16QF_04820"
     CDS             <1..3887
                     /locus_tag="AC16QF_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6447908.1"
                     /translation="YTQALARALGDVADKRNPRLRILGYTRNERLDRRTASVYGDDIG
                     LSAARARRAMETLSAEMGLAPEQAEFEGRGYVHSADVVNAGFVQDERSYVAVQVVYDD
                     LAVVDDTEGVEVTPLTRELETKNPYALNLMRITVDGEPIDDPNRSSADVQRCTDVAMK
                     GADIRFGFDNLSSKPRLSVSASTAILGVTEAAEDSLSASGLKFSMYANYSHFIDRSEI
                     RIFDRDASTQTEPMAVIAVGPDGQATWEPVLDSFAGPVRELKYLLRAYDAQDRFDETT
                     TQPLWLVRETGSAAAAEGPVAEAEEGEPTDPLQPELLSGYGENALAMHNIRLSSGTVR
                     VSGSEVPEGHRVYVAGREVPVDAKGNFVSEEILPEGAHTVEVAVLDDQGSGESYLRDL
                     AFEPSDWFYVGMADLTFSHNETNGPMELLMGDDAPYDYDSSADGRLAFFVSGKFGNKW
                     RLTASADTREDSLDKLFTNFLSKTPDSLFRRMEPDDHYPTFGDDGTVEELAPTLGKFY
                     VKLSQRENHVMWGNFKVGYMDNELAQIDRGLYGANFHYQAADTTSFGEQKFSVDAFAA
                     EPGTVASRQEFRGTGGSLYFLRHQDLMVGSERVRVEVRDRSSGLVTGVVNLNPGSDYD
                     VDYLQGSIMLAEPLASTVGDDLLVRSGALGGDEAYLVVRYEYTPGFDEIDALSVGGQM
                     HYWINDHIKVGLTANANEEGGTDSGLHGADLTLRKSADSWVKLQVSQSEGLVTDTLRS
                     SDGGFEFAGYDPSSFSDASAGGYRADVSVGLDDFSSRLDGRITLYAQDLDAGYSGPGL
                     TTVGGARNFGGTFTMPVTRKLEVSAKSDLRKQRQGVETTAHELDLVYDLTQRWDISAG
                     VRHDELTDRSPVVAPTQDQGQRIDAVMQVGYDSLGRWKAYGFVQDTVSSDGDRPDNAR
                     LGLGGQVRVTEKMTIDSEFSTGDLGTGGKLGTTYMHSERTSLYLNYAMDNERTEPMLR
                     APRGSEGNLVSGIKTRLSDATSVYVESRYRHGDTANGLTYASGVNFTASNSFNIGANT
                     DIGTLKDLSTGAETDRKAAGVRVAFGTERLQLSSGIEYRSDDTEQLDDTTSNRKTWLF
                     RNTLKYQLNESSRLVGKLNHADSTSSLGNFFAGGYTEAVFGYAYRPVRHDRLNTLMKY
                     TYFYNVPTTDQVSLTQTAADFVQKSHIASVDVTYDLTSRWSIGGKYAYRLGEISYDRE
                     NPEFFSNTASLYVVRLDWEFRENWEIMAETRLLDMADFQEQRQGYLVAVSRNFGDHFK
                     VGAGYNFTDFSSDLTDLSFDHQGMFLNLTGAF"
     gene            complement(3913..4170)
                     /locus_tag="AC16QF_04825"
     CDS             complement(3913..4170)
                     /locus_tag="AC16QF_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447909.1"
                     /translation="MTEIFDEDDEEGLSPHILEQVRRLQARIDRRESLRREHVARSRG
                     LRRRLEDWEEQRALRSSVDYLEMEFAERDGDRRTGRAALPR"
     gene            4291..5133
                     /locus_tag="AC16QF_04830"
     CDS             4291..5133
                     /locus_tag="AC16QF_04830"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6447910.1"
                     /translation="METELLRTFLEVNRTRHFARAASNLHLTQAAVSARVRHLEEIVG
                     ERLFTRTRNNIQLTVAGHRLLGHAEAIVGSWNRALLETGAPASTRALVALGCLPSIWE
                     TYLTDWLSRTYEDLSPILLQIELLNTEALVRRLRENSITIGLLYEPPQADDLIAEQVT
                     EVSVRLVSSSQGLSVSDSLPGYVYVDWGTSFAIAHDTRLGISTPPALRVDTPAIAYRF
                     LTEHGGTAYLPSAMVAGALSAGLLFPVAGGKTFTRAAFIARSATASAVDSIKAVTASL
                     RALV"
     gene            complement(5096..7420)
                     /gene="ptsP"
                     /locus_tag="AC16QF_04835"
     CDS             complement(5096..7420)
                     /gene="ptsP"
                     /locus_tag="AC16QF_04835"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF008283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR6447911.1"
                     /translation="MTMPTMGGKRPENRVSLQLLQRILVDASSAHTVEEQVERLVSQV
                     RDAMAAGACSLYQRFDDTLRMVANCGFAPESVGRIALPMGEGLVGRIARDRMSLNLAD
                     ASAHPDFRLFPQSGEEAFPAFLGVPIIHLGAVNGVLIVQDDRPSAFSEDEVSFLVTVA
                     AQLGPILLPLRAPASTSGRRLRGRSGSPGKAVGRVHLVLSEQTVQLVEEPQSAGTEEE
                     IGVLETAVSRTREKIGRAKSQLEAHVAPDVLELFDLYNAMLSGDRLVTAAAGRVREGH
                     SAFSAVRATVDETIAAFEAIEDDYLSARAEDVRHVGNKLLVEILGSAMPSPEHAEGVV
                     LLGGSVGIADVGAFRPEQLAGIVCLQGSSYSHTAVLARALGIPAVVGIGPVDHIVEGM
                     PVIVDGDGGEVIFEPPGVLLRAYEELVASERQLQNELLAQKDLPGVTTDGCRITLLAN
                     TGLMADVRPGKLRGAEGIGLYRSEIPFLMSPVLPTEAEQYATYRELLEIYHPLPVTIR
                     TVDLGGDKQLPYLPTQEENPALGWRGIRFALDNPAILVSQLRAMLRADHGLGNLQIML
                     PMISDVSEVQEARRLANTVAAALTAEGLDIRMPPLGVMVEIPAVVDVLPDLAPEIDFA
                     CVGSNDLAQYLLAVDRGNPRVVGRYDYLHPGVLGCLDVIARRVEDLGIRLSICGEMAA
                     DPVGVVVLLGLGYRALSMNAYSLPRIRALIRGISIREAQALARSALACRNPGSSRVAI
                     AAALAGLGLDHLLAHGGAFESASTPALAGSPSRP"
     gene            7646..8203
                     /locus_tag="AC16QF_04840"
     CDS             7646..8203
                     /locus_tag="AC16QF_04840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6447912.1"
                     /translation="MSHESQRRVSLADLSDEALAASARHQLPADTSAYDELVRRHYES
                     VRRLATAQLGAPDEADALAQDVMLRVFSGLKNFRGDAKFSTWLFRIVENLARTRVRQQ
                     IREQDKREALGMAQHEREEDAPDQSDFQRLLAVLSPQERALLALRFVEDMELHEIAAV
                     LGLSLSATKMRYYRTLDKLKDHIPG"
     gene            8394..9974
                     /gene="groL"
                     /locus_tag="AC16QF_04845"
     CDS             8394..9974
                     /gene="groL"
                     /locus_tag="AC16QF_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155568.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR6447913.1"
                     /translation="MSAKQIVFAHDARARLNRGVDTLARAVNATLGPRGRNVLLSKTW
                     GAPTVTKDGVTVAKDIELKDRFEDMGARIVRQAATRTADEAGDGTTTATVIAHSLFHW
                     GRRAIAAGFSPVEIKRGIDLGVRLMTDALDAMAVECEAGDDIRKVATISANGDEAIGE
                     LIAQAMEQVGREGVITVKDGTGIDDQLEVSEGMQFDQGYLSPYFVTSTETYAAELDRP
                     VILLRDKKISSSRELLPLLEAVARANRSLLIVAETVEADALATLVVNRLRGRLKVAAV
                     KAPGFGDRRKAMLQDLAILTGGKVISSDAGFSLEKVALEDLGEAQRVLLTKGDTTLIG
                     GRGGTDAIAGRISQIRREIEESKSEYDREKLQERLAKLAGGVAVINVGGATEVAMKER
                     KMRVDDALAATRAAVEEGVVPGGGVALLRASAELAKAAPRNQDQASGVDALRRAAEAP
                     LRQIVANAGLQPGVVLRKIQSEQGHFGFNAQTETFGDMLEMGILDPCKVVKSALHNAA
                     SVAGMMMSTEALVADAPR"
     gene            complement(10009..10548)
                     /locus_tag="AC16QF_04850"
     CDS             complement(10009..10548)
                     /locus_tag="AC16QF_04850"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6447914.1"
                     /translation="MTSPCKEHPPGDAWVHLREPAARDRGPVASVLVSTGFFSDAEVA
                     VALEVFDAALQGKDSGYEHLVAESAGRVIGYVCWGGPIEMTLSSFDLYWIAVDAAARG
                     RGVGHRLLAAAESLAAASGCAQLFAETSGRADYAPTHSFYESCGYLRAAELADFYAPG
                     DAKVFYVKRLTGGNQAPPE"
     gene            complement(10551..11537)
                     /locus_tag="AC16QF_04855"
     CDS             complement(10551..11537)
                     /locus_tag="AC16QF_04855"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR6447915.1"
                     /translation="MRVAIAYNKPGDAAGSAEADVLEQVEAVETALVGAGHQVCALPC
                     TLDLAGFDGALAKQRPDVVFNLVEALGGTDRLAVLVTLLLDARGVPYTGAGSRALELS
                     ADKVGAKERMRRLGLPTPDWYVCEKTQVDFPGAGCYIVKARFEHASLGLDDQSVVQVA
                     DAGQLTRRIRARGKHSGAQQFAERYVAGREFNVALLASETGPAQVLPPAEIDFSAFAP
                     GKPRIVGHDAKWSSRAFEYHATPRRFDFPAADARLLDLLGTLGMQVWECFAMRGCARV
                     DFRVDEDGRPWILEINPNPCLSPDAGFAAALDAAGIPAEEMVGRLVRTAATD"
     gene            complement(11528..12541)
                     /locus_tag="AC16QF_04860"
     CDS             complement(11528..12541)
                     /locus_tag="AC16QF_04860"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR6447916.1"
                     /translation="MRIGITYDLRSAYLAAGYSEEETAEFDRDDTVDAIEAALRQLGH
                     ETVRIGCARELIEHLAAGQRWDLVFNICEGLSGPGRESQVPAILEVYGIPVTFADTAV
                     MALSLNKAWAKTVVADAGVATPQHAVIARAADVATVALEPPLFVKPIGEGTGKGTSAA
                     SVVMSAAELERQSLALLERYRQPVLVETYLPGREFTVGLLGTGPNARVLGTLEIELLA
                     TAEANVYSYLNKEECESRVLYRLVDGRADPVVARAEAAALGAWRALGARDGGRIDLRA
                     DSAGEPNFIEANPLAGLHPAHSDLPMLATAVGMPYAELIRAIVDSASERLAMDATEMN
                     RCA"
     gene            complement(12544..>13822)
                     /locus_tag="AC16QF_04865"
     CDS             complement(12544..>13822)
                     /locus_tag="AC16QF_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HIR6447917.1"
                     /translation="TAPADDARVIKVIPAKSTDSTSVASKGVESVQRRAKVVRLRTAG
                     RGNPRTREFRIRHFPDVSDAEWNDWRWQSRHRFKNLAQIERVLELSDDERAALTVGGM
                     MLPVGITPYYMSLLDPVDPQHALRRTVIPSQNEFLRLPGEADDPLGEDAHSPVPGLVH
                     RYPDRVLMLPIDFCSTYCRYCTRSRVVGHGELSPQTSRLEAMFDYLRGATQVRDVLVS
                     GGDALALGEDNLDYILSNLRAIEHIEFVRIGTKMPAVLPQRITRDLTRVLRKYHPLWM
                     SLHFLHPEECTPESTQACERLADAGIPLGSQTVLLKGVNDDVGVMTRLVHGLLKMRVR
                     PYYLYQCDPISGSAHFRTTVEKGLEIIEGLRGHTTGYAVPTYVIDAPGGGGKIPVQPN
                     YVLGRDVDELKLRNFEGRVFSYPDPQQPGAGTN"
BASE COUNT         2495 a         4647 c         4492 g         2188 t
ORIGIN      
        1 gctacaccca ggccctggcc cgcgccctcg gcgacgtggc cgacaagcgc aacccccgcc
       61 tgcgcatcct cggctacacg cgcaacgagc gcctggaccg ccgcaccgcc tcggtgtacg
      121 gcgacgacat cggcctgtcc gctgccagag cgcgacgggc catggaaacg ctttcagcgg
      181 agatgggcct ggctccagag caggccgagt tcgaaggccg cggctacgtg cactcggcag
      241 acgtggtcaa cgccgggttc gtccaggacg aacgctccta cgttgccgtc caggtcgtct
      301 acgacgatct cgccgtggtc gacgacaccg agggagtgga ggtcacgccg ctcacccggg
      361 agctggaaac gaagaacccc tatgcgctca atctcatgcg catcaccgtg gacggcgagc
      421 ccatcgacga cccgaaccga agctccgccg acgtccagcg ctgcacggac gttgccatga
      481 aaggcgccga catccgcttc ggcttcgaca acttgagctc gaagccgcgg ctgagcgtgt
      541 ccgccagcac cgcgatcctg ggggtcacgg aagctgccga agattccctc tccgccagcg
      601 ggctgaagtt cagcatgtac gcgaactact cccacttcat cgaccgctcg gagatccgca
      661 tcttcgaccg ggacgcctcc acccagaccg agcccatggc cgtgatcgcc gtgggtccgg
      721 acggccaggc gacctgggag cccgtgttgg attcgtttgc cggcccggtg cgggagctga
      781 agtacctgct gcgcgcctac gatgcgcagg accgcttcga cgagaccacc acgcagccct
      841 tgtggctggt gcgtgaaacc ggcagcgcgg cggctgccga aggcccggtc gcagaggcag
      901 aggaaggcga gcccaccgac ccgctccagc cggagttgct ctccggctac ggcgagaacg
      961 ctctcgccat gcacaacatc cgtctttcga gcggcacggt tcgggtcagc ggcagcgagg
     1021 ttcccgaagg ccaccgcgtt tacgtcgcgg gtcgggaagt gcccgtggac gccaaaggca
     1081 acttcgtcag cgaggaaatc ctgccggagg gcgcccacac cgtggaggtg gccgttctcg
     1141 acgatcaggg cagcggggag tcctatctgc gggatctggc gttcgagccc agcgactggt
     1201 tctacgtggg catggcggat ctgacgttct cccacaacga gaccaacggc cccatggaac
     1261 tgctcatggg ggacgatgca ccgtacgact acgactcgtc cgccgacggc cggctggcct
     1321 tcttcgtcag cgggaagttc ggcaacaagt ggcgcctcac ggccagcgcc gacacccgtg
     1381 aggattccct ggacaagctc ttcacgaact tcctgagcaa gacgcccgac tccctcttcc
     1441 gccgcatgga gccggacgat cactatccca ccttcgggga cgacggcacg gtggaggagc
     1501 tcgccccgac cctcggcaag ttctacgtga agctcagcca gcgcgagaac cacgtcatgt
     1561 ggggcaattt caaggtcggc tacatggaca acgagctcgc gcagatcgac cgggggctgt
     1621 acggcgcgaa cttccactac caggccgccg acaccaccag cttcggcgag cagaagttca
     1681 gcgtggatgc tttcgccgcc gagcccggca cggtggcgag ccgccaggag ttccgcggca
     1741 ccggcgggtc cctctacttc ctgcgccatc aggatctgat ggtgggctcc gagcgggttc
     1801 gggtcgaggt gcgggaccga tcctccgggc tcgtcaccgg cgtggtgaac ctcaaccccg
     1861 gctccgacta cgacgtcgac tacctccagg gcagcatcat gctggccgag cccctggctt
     1921 ccacggtggg cgacgacctg ctcgtgcgca gcggcgcgct gggcggcgac gaggcctatc
     1981 tggtggttcg ctacgagtac acgcccggct tcgacgagat cgacgccctg tccgtgggcg
     2041 gccagatgca ctactggatc aacgaccaca tcaaggtggg gctcaccgca aacgccaacg
     2101 aggaaggcgg cacggacagc gggctgcacg gtgccgacct caccctgcgc aagagcgcgg
     2161 actcctgggt caagctgcag gtgtcgcaga gcgaggggct cgtcaccgac accctgcgct
     2221 cgagcgacgg cggcttcgag ttcgccggct acgacccctc gtcgttctcc gatgccagcg
     2281 ccggcggcta ccgggccgac gtgagcgttg gcctcgacga cttctcgtcc cgcctggacg
     2341 gccgaatcac cctgtacgcc caggatctgg atgccggcta ctccggcccc ggtctgacca
     2401 ccgttggcgg cgcccgcaac ttcggcggca ccttcaccat gcccgtgacg cgcaagctgg
     2461 aagtcagcgc caagagcgat ctgagaaagc agcgtcaggg cgtggagacg acggcccacg
     2521 agctcgacct ggtatacgac ctgacgcagc gatgggacat cagcgccggc gtgcgacacg
     2581 acgagctcac cgaccgctct cccgtggtgg cgcccaccca ggatcagggc cagcgcatcg
     2641 acgccgtgat gcaggtgggc tacgactccc tcggccgctg gaaggcctac ggtttcgttc
     2701 aggatacggt ttcctctgac ggcgacaggc cagacaacgc acgcctaggc ctcggcggcc
     2761 aggtgcgcgt cacggagaag atgaccatcg actccgagtt ttccaccggc gatctcggca
     2821 cgggcggaaa gctcggcacg acgtacatgc attcggagcg gaccagcctt tacctgaact
     2881 acgccatgga caacgagcga accgagccca tgctgcgcgc gccccggggc agcgagggca
     2941 acctggtttc agggatcaag acgcggctgt ccgacgccac gagcgtgtac gtggagagcc
     3001 gctaccggca cggcgatacg gccaacggcc tcacctacgc ctccggcgtg aacttcaccg
     3061 cgtccaacag cttcaacatc ggcgcgaaca cggacatcgg cacgctgaag gatctgtcca
     3121 cgggagcgga gaccgaccgc aaggccgccg gcgtccgggt cgctttcggc accgagcgcc
     3181 tgcagctctc cagcggcatc gagtaccgga gcgacgacac cgagcagctg gacgacacca
     3241 cctccaaccg caagacctgg ctgttcagga acacgctgaa gtaccagctg aacgaaagct
     3301 cccgtctcgt gggcaagctg aaccacgccg acagcacgag ctcgctgggc aacttcttcg
     3361 ccggcggcta caccgaggcc gtgttcggct atgcgtaccg accggtgcgt cacgacaggc
     3421 tcaacacgct gatgaagtac acgtacttct acaacgtccc gaccacggat caggtctcgc
     3481 tcacccagac cgcggcggac ttcgtccaga agagccacat cgcgtccgtg gacgtcacct
     3541 acgatctcac ctcacgctgg tccatcggcg gcaagtacgc ataccgcctc ggcgagatca
     3601 gctacgatcg ggaaaacccc gagttcttca gcaacaccgc cagcctctac gtggtccggc
     3661 tggactggga gttccgcgag aactgggaaa tcatggccga gacgcgcctg ctggacatgg
     3721 ccgattttca agagcagcgc cagggctatc tcgttgccgt ctcgcgcaac ttcggcgatc
     3781 acttcaaggt cggcgccggc tacaacttca cggacttctc gtccgacctg acggatctga
     3841 gcttcgatca tcagggcatg ttcctcaacc tcaccggcgc gttctagccg tgaagtcgtg
     3901 cgggccagcc ggtcagcgtg gcaaggcggc tcggccggtg cggcgatctc cgtcgcgctc
     3961 ggcgaattcc atttccaggt agtcgacgct cgagcgaagg gcccgctgct cttcccagtc
     4021 ttccagccgg cgccgcagtc cccggctgcg cgccacgtgc tcgcgcctca agctttcccg
     4081 ccggtcgatg cgggcctgca gccgtcgcac ctgctcgaga atgtgaggcg ataacccttc
     4141 ctcgtcgtct tcgtcgaaaa tttcagtcat gtcgctctcg atgcggggcc gcaacgcccc
     4201 ggataacgtc ggcgtatata tacgcccggc tatttggcaa atacatcaaa tatttctgta
     4261 ctttttgata acagatgttt atcaaaaggc atggaaaccg agctgctcag aactttcctg
     4321 gaagtcaatc gcacccgcca cttcgctcgc gccgccagca acctgcacct gactcaggcg
     4381 gccgtgagcg cgcgggtgcg ccatctcgag gaaatcgtcg gcgagcggct gttcacccgc
     4441 accagaaaca acatccagct gaccgtcgcc gggcaccggc tgctcggaca tgccgaggcg
     4501 atcgtcgggt cctggaaccg ggccctcctc gaaaccggcg ccccggcgag cacgcgggcg
     4561 ctggtggcgc tgggttgtct gcccagcatc tgggaaacgt atctgaccga ctggctgagc
     4621 cgcacctacg aggacctgtc gccgatcttg ttgcagatcg aactgctcaa caccgaggcg
     4681 ctcgtcaggc gcctgcggga gaactccatc accatcgggc ttctctacga accgccccaa
     4741 gcggacgatc tgattgccga gcaggtcacc gaggtcagcg taaggctggt ctcgtccagc
     4801 caggggctca gcgtctcgga ctctctgccc ggttacgttt acgtcgactg gggaacgtct
     4861 ttcgccatcg cgcacgatac gcgcctgggg atttcaaccc cgcccgcgct gcgggtcgac
     4921 acgccggcta tcgcctaccg ctttctcacc gagcacggcg gaacggcgta tctgccgtcg
     4981 gccatggtcg ccggtgccct gtccgcaggg ctgctgtttc ccgtcgcagg agggaagacg
     5041 ttcacgaggg ccgcgttcat cgcgaggagc gcgaccgcca gcgcggtcga ttcgatcaag
     5101 gccgtgacgg cgagcctgcg agcgctggtg tagacgccga ctcaaacgcc cccccatggg
     5161 ccagcagatg atccagcccg agcccggcca gcgccgcggc gatggccacg cggctcgacc
     5221 cgggattacg gcaggccaac gcactgcggg ccaacgcctg tgcctcacgg atcgatatgc
     5281 ctcgaatcag cgccctgata cgcggaaggc tgtatgcgtt catgctcagc gcccgatagc
     5341 cgagcccaag cagcacgacc accccgacgg ggtcggccgc catctccccg cagatcgaca
     5401 agcggatgcc gagatcttcg acgcgccgcg cgatcacgtc gaggcagccg agcacgccgg
     5461 gatgaaggta gtcgtagcgg ccgaccaccc gggggttgcc gcggtcgacg gcgagcaggt
     5521 actgagccag atcgttggaa ccgacacagg cgaagtcgat ctccggggcc aggtccggca
     5581 gcacgtcgac caccgcagga atttcgacca tgacgcccag cggaggcatg cggatgtcga
     5641 ggccttccgc cgtgagcgct gccgcgaccg tgtttgccag gcgtctcgcc tcttgcactt
     5701 cgcttacgtc gctgatcatc gggagcatga tctgcagatt gccaaggcca tgatcggcgc
     5761 ggagcatggc gcgcagttgg gagaccaaga ttgccggatt gtcgagggcg aagcgaatgc
     5821 cccgccatcc cagcgcaggg ttctcctcct gggtcggcag gtaaggcagc tgcttgtcac
     5881 caccgaggtc cacggtgcga atggtcaccg gcaacggatg ataaatctcg agcagctcgc
     5941 gataggtcgc atactgctca gcctcggtag gcaggacggg gctcatcagg aacgggattt
     6001 ccgagcgata caggccgatg ccttccgcgc cccgcagctt gcccggccgg acatcggcca
     6061 tgagccccgt attcgccagc agagtgattc ggcacccgtc cgtcgtgacg cccgggagat
     6121 ccttctgcgc cagcagttcg ttctgcagct gacgttcgga ggcgacgagc tcctcatagg
     6181 cccgcagcag cacgccagga ggctcgaaga tcacctcgcc gccatcgccg tcgacgatca
     6241 cgggcatgcc ctcgacgatg tggtcaaccg gaccgatccc gacgacggcg gggatgccca
     6301 gcgcccgggc aagcacggcc gtgtgagagt acgaagagcc ctgcaggcag acgattccgg
     6361 ccagctgctc gggcctgaac gcgcccacgt cggctatccc caccgagccg ccgagcagca
     6421 cgacgccctc ggcatgctcg ggcgacggca tggcgctgcc caggatctcc acgagcagct
     6481 tgtttccaac atggcgcacg tcctcggcac gggcgctgag atagtcgtct tcgatggctt
     6541 cgaatgcggc aatggtctca tcgaccgtcg cgcgaaccgc cgagaacgcg ctgtgccctt
     6601 cacgcacccg ccccgccgcg gcggtcacca gccggtctcc ggacagcatg gcgttgtaca
     6661 gatcgaagag ctccagcacg tcgggggcca catgggcctc gagctgggac ttggcgcgcc
     6721 cgatcttctc tcgggtccgc gacacggcag tttccaggac gccgatctcc tcttcggtgc
     6781 cggcagattg cggttcctcg accagctgca cggtctgctc gctgagcacc aggtgaacgc
     6841 gccccacagc cttgccgggc gaccccgacc gcccgcgcag cctgcgcccg gacgtcgacg
     6901 ccggcgcgcg cagcggcagc agaatgggtc caagctgggc ggctacggtc accaggaaag
     6961 agacctcgtc ctcgctgaac gccgagggcc gatcgtcctg cacgatgagc acgccgttga
     7021 cggcgcccag atggatgatc ggcaccccga ggaaggcggg aaacgcctct tcaccggact
     7081 gcgggaacag acgaaagtcg ggatgggccg aagcatcggc caggttgagc gacatccggt
     7141 cccgggcgat cctgccaacg agcccctcac ccatcggcag cgcgatacgc ccgacggact
     7201 cgggagcgaa cccgcagttg gctaccatcc tgagagtgtc gtcaaagcgc tgatacaggc
     7261 tgcaggcgcc cgctgccatg gcgtcacgga cctgcgaaac cagccgctcg acctgctcct
     7321 ccacggtgtg cgccgagctg gcatcgacga gaatccgctg caggagctgc agggaaacac
     7381 ggttctcggg ccgcttaccc cccatcgtcg gcatcgtcat cgccggctcg cggcggtccg
     7441 cctgccggtt gtcgtcggca ttgctcggac ctcttggcgg cagcgcccag caatcgcccg
     7501 aggcgcactc tgagcgcacg cgccgcgaac ccatgagatt ccggcatggc ctgctcgtaa
     7561 agcgttctca ggccggcttc gagtttcgaa cgatctttca cgggcgccta tttgtccacg
     7621 gctccataga cattagactg cgcgcgtgag ccacgagtca cagcgccgcg tttcccttgc
     7681 cgacctctcc gacgaggcac tggcggcgag tgcccgtcac caattgcccg ccgacacttc
     7741 ggcctatgac gaactggtca ggcggcacta cgaatcggtg cgcaggctgg ccacggcgca
     7801 gctcggcgct cccgacgagg ccgatgccct tgcccaggac gtcatgctgc gggtgttctc
     7861 cggcctgaag aacttccgag gcgacgcgaa gttttccacc tggctgttca ggatcgtgga
     7921 gaacctggcc cgcacccggg tccgacagca gatacgggaa caggacaagc gggaagcgct
     7981 gggcatggcg cagcacgagc gggaggaaga cgctcccgac cagagcgact ttcagcgcct
     8041 gctggccgtg ctttctccgc aagagcgcgc gctgctcgcg ctgcgctttg tggaggacat
     8101 ggagctgcac gagatcgcgg ccgtgctagg actcagcttg agcgcgacga agatgcgcta
     8161 ctaccgcacc ctggataagc tcaaggacca cattccgggc tgattttcat cgcctgctgt
     8221 gactttttcg actggccgcg agtctgatct gaagattttt tgcacggccg gatccgatcg
     8281 atgattaccc gtgaggagtc cctcctcata gcacaggaac tgcgcaaccg acaggcggcg
     8341 gcgaagtgcg ggcgctcggt tgccggccgc aaccggcacc cgaggaaaac cgcatgagcg
     8401 ccaagcagat cgtttttgcc catgacgcaa gagcccggct caaccggggc gtcgacacct
     8461 tggcccgggc cgtcaacgcc accttgggac ctcggggacg taacgtgctg ctgagcaaga
     8521 cctggggcgc accgacggtg accaaggatg gcgtcaccgt cgccaaggac atcgagctga
     8581 aggaccgatt cgaggacatg ggcgcgcgaa tcgtccgcca ggcggcaacg cggaccgcgg
     8641 acgaggccgg ggacggcacc acaacggcca cggtgatcgc tcactctctc tttcactggg
     8701 gccgtcgcgc cattgcggca ggattcagcc ccgttgaaat caagcgcggc atcgacctgg
     8761 gcgtgcgcct gatgaccgat gccctggacg cgatggccgt cgagtgcgag gccggggacg
     8821 acatacgcaa ggtggccacg atttctgcca acggcgacga ggccatcggc gagctcatcg
     8881 cgcaggccat ggagcaggtg ggtcgcgagg gtgtgattac cgtcaaggac ggcaccggca
     8941 tcgacgacca gctcgaagta tctgagggca tgcagttcga tcagggttac ctctcaccct
     9001 acttcgtcac cagcaccgag acctatgctg ccgagctcga tcggccggtg atcctgctgc
     9061 gggacaagaa gatctccagc tcccgcgaac tgctgccact gctggaggcg gtcgcccggg
     9121 ccaaccgctc gctgctcatc gtcgccgaga ccgtcgaggc tgacgcgctg gcgaccctgg
     9181 tcgtcaacag gctgcgcggc cgcctgaagg ttgccgcagt caaagcgccg ggcttcggtg
     9241 accggcgcaa ggccatgctg caggacctgg cgatcctcac cggcggaaag gtcatctcca
     9301 gcgacgcggg attcagcctg gaaaaggtgg cgctggagga tctcggcgag gcgcaacggg
     9361 tattgctgac caaaggggac acgacgctca tcgggggtcg cggcgggacc gacgctatcg
     9421 ccgggcgcat ctcgcagatc cgtcgcgaga tcgaggagtc caagtccgag tacgaccgcg
     9481 aaaagctgca ggagcgtctt gccaaactgg caggcggcgt ggcggtgatc aacgtcggcg
     9541 gcgccacgga agtcgccatg aaggagagga agatgcgcgt ggacgacgcg ctcgctgcta
     9601 ctcgcgctgc cgtggaagag ggcgtcgtgc cgggaggcgg cgtggcactg ctgcgcgcct
     9661 cagccgagct cgcaaaggcc gcgccccgaa accaggacca ggcaagcggc gtggacgcgc
     9721 ttcggcgggc tgccgaggcg cccctgcgcc agatcgtcgc caacgccggt ctgcagccag
     9781 gcgtggtgct gagaaagatt cagtcggagc agggacactt cgggttcaac gcgcagaccg
     9841 aaacgttcgg cgacatgctg gaaatgggca tactggaccc ctgcaaggtc gtgaagtcgg
     9901 cgctgcacaa cgcagcgtcc gtggccggca tgatgatgtc gacggaggcg ctggtcgcag
     9961 acgcgccccg ctagagaggg ccgcgctcgg aacacggtcg gaacgccgtc attccggtgg
    10021 ggcctgattg ccgccggtca gccgtttgac gtagaaaact ttggcgtcac ctggcgcgta
    10081 gaagtccgcg agctcggccg cgcgcaggta gccgcaggat tcgtagaagc tgtgcgtggg
    10141 cgcgtaatcg gcacgtccgg aggtctccgc aaaaagctgg gcacagcccg acgccgccgc
    10201 caggctctcg gccgcggcca gcagccgatg accgaccccg cggccccggg ccgccgcatc
    10261 cacggcgatc cagtaaaggt cgaagctcga gagcgtcatc tctatgggcc cgccccagca
    10321 gacgtacccg atcacccttc cggcggattc ggccacgaga tgctcgtagc cggagtcctt
    10381 accctgcaat gccgcatcga agacttccaa cgccacagcg acttcggcat cactgaaaaa
    10441 tcccgtcgag accagcaccg aggccacggg tccacggtcg cgtgccgccg gctcccgcag
    10501 atgaacccag gcgtccccgg ggggatgctc tttgcagggt gaggtcatgc tcagtccgta
    10561 gcggccgtcc tgaccagtcg cccgaccatc tcctccgccg ggatcccggc cgcatcgagc
    10621 gcggcagcga agccggcatc gggcgagaga cacggattcg ggttgatctc cagaatccag
    10681 ggtcggccgt cctcgtcgac ccggaagtcg acgcgggcgc agccgcgcat cgcgaagcac
    10741 tcccagacct gcatcccgag agttccaagc aggtcgagca accgggcatc ggctgcgggg
    10801 aagtcgaaac gccgcggggt cgcatgatac tcgaaggcgc ggctcgacca cttggcgtca
    10861 tgcccgacga tgcggggttt gcccggcgca aacgccgaga agtcgatctc cgccggaggc
    10921 agcacctggg cggggccggt ttccgaagcc agaagcgcca cgttgaactc acgcccggca
    10981 acgtagcgtt cggcgaactg ctgcgcgcca ctgtgctttc cgcgtgcgcg gatgcgcctg
    11041 gtgagctgcc cggcatcagc aacctgaacg accgactggt cgtccaggcc aagggaggcg
    11101 tgctcaaagc gtgccttgac gatgtagcac cctgccccag ggaaatccac ctgggtcttc
    11161 tcacagacgt accagtccgg cgtcggcagg ccgagcctgc gcattcgttc cttggcgccg
    11221 accttatcgg cgctgagctc cagcgcccgc gatcccgctc cggtgtaggg gacaccgcgg
    11281 gcgtcgagga gcagcgtcac cagcacggcc aggcggtcgg ttccgcccag ggcctcgacc
    11341 agattgaaga cgacgtcagg ccgctgcttt gccagggcac catcgaatcc ggcgagatcc
    11401 agcgtgcagg gcaacgcgca gacctggtga ccggcgccca cgagggcagt ctcaaccgct
    11461 tcgacctgct cgagcacgtc ggcttccgcg ctgcccgcag cgtcgcccgg cttgttgtaa
    11521 gcgatggcta cgcgcaacgg ttcatttccg tagcgtccat ggcaagacgt tccgaagccg
    11581 agtcgacgat ggcacgaatg agctctgcat aaggcattcc caccgcggta gccagcatgg
    11641 gcagatcgga atgagcggga tgcagccctg ccagggggtt tgcctcgatg aaattgggtt
    11701 cccccgccga gtccgcgcga aggtcgatgc gccctccgtc ccgggccccg agggcccgcc
    11761 aggcgccgag cgccgcggcc tcggcccggg caacgacggg atcggcacga ccatcgacga
    11821 gtcggtacag cacacgggac tcgcactcct ccttgttgag gtacgagtag acgttcgcct
    11881 cggcagttgc caggagctcg atctcgagag tcccgagcac cctggcattc gggccagtgc
    11941 ccagaaggcc gacggtgaac tctctgccag ggaggtacgt ctcgaccagc acgggctgcc
    12001 ggtagcgctc gagcagggcc aggctctgcc gttcaagctc ggcggcgctc atgacgaccg
    12061 aagcggcgct tgtgcccttg ccggtgccct ccccgatcgg cttgacgaac aggggcggct
    12121 cgagcgcgac cgtggcaacg tcagcggccc gggcgatgac ggcatgctgc ggggtcgcca
    12181 caccggcgtc ggccaccacc gtcttggccc aggccttgtt caggctcaga gccatgacgg
    12241 ctgtatcggc aaacgtcacg ggtatgccgt agacctccag aatggctggc acctggctct
    12301 cccgcccggg accgctgagc ccctcgcaga tgttgaacac cagatcccaa cgctgtcccg
    12361 cggcaaggtg ctcgatgagc tcgcgggcgc agccgatgcg caccgtctcg tgcccgagct
    12421 ggcgcaacgc cgcctcgatg gcgtcgacgg tgtcgtcgcg gtcgaactcc gccgtttcct
    12481 cctccgagta gccggcggcg aggtacgcgg aacgcagatc gtaggtgata ccgatgcgca
    12541 tgttcagttg gtcccggcgc caggttgctg gggatccgga tagctgaaga cccgtccctc
    12601 gaaattgcgg agtttgagct cgtcgacgtc tctgccgagc acgtagttcg gctgaaccgg
    12661 aatcttgccc ccaccgccgg gcgcatcgat gacgtaggtc ggcacggcgt aacccgtggt
    12721 gtggccgcgc agcccttcga tgatctccag gcccttctca acggtcgtgc ggaagtgggc
    12781 cgaaccactg atcgggtcgc actggtacag gtagtagggc cgcacccgca tcttcaggag
    12841 gccgtgcacg aggcgcgtca tcacgcccac gtcgtcgttc acgcccttga gcagaacggt
    12901 ctgggagccg agcggtatac cggcatcggc cagccgctcg caggcctggg tggactccgg
    12961 cgtgcattcc tccgggtgca ggaagtgcag gctcatccag agcgggtggt acttgcgaag
    13021 caccctggtc agatcccggg tgattcgttg cgggagcacg gccggcatct tggtgccgat
    13081 gcggacgaat tcgatgtgct ctatggcccg caggttggaa aggatgtagt cgaggttgtc
    13141 ctcgccgagg gcaagcgcgt cgccgccaga gacgagcacg tcgcgaacct gggtcgcgcc
    13201 tcgcaggtaa tcgaacatgg cttccaggcg cgatgtctgg ggggagagct ccccatggcc
    13261 tactacccgc gagcgagtgc agtagcggca gtaggtggaa cagaaatcga ttggcagcat
    13321 cagcacccgg tcggggtaac ggtgcacgag ccccggcacc ggactgtggg cgtcctcccc
    13381 gagcgggtcg tcggcttccc ccggcaggcg aagaaattcg ttctgcgagg gtatgaccgt
    13441 ccgccgcagg gcatgctgcg gatcaacggg gtcgagcaga ctcatgtagt agggcgtgat
    13501 ccccacgggc agcatcatgc cgccaacggt gagcgctgcg cgctcatcgt cggacagttc
    13561 gagcacgcgc tcgatctggg cgagattctt gaagcggtgc cgggactgcc agcgccagtc
    13621 gttccactcc gcatcggaaa cgtccgggaa atggcggatc cgaaactcgc gtgtgcgggg
    13681 gttgccgcgc cctgcggtcc gcagccggac taccttcgcc cgacgctgca cggactcgac
    13741 gcccttcgat gcaacggatg tcgagtcggt ggactttgca gggatgactt tgatgacacg
    13801 cgcgtcgtcg gctggcgcgg ta
//
LOCUS       DBNHLM010000105         2282 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_374374, whole genome shotgun sequence.
ACCESSION   DBNHLM010000105 DBNHLM010000000
VERSION     DBNHLM010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_374374"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..497
                     /locus_tag="AC16QF_04870"
     CDS             165..497
                     /locus_tag="AC16QF_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447918.1"
                     /translation="MNRLLKLPLYFCLSALPAATSVAQEGDKAMSGMSILGNNDAPKA
                     LVIVPWKSSEIGDGIDVANLLDGRARAVDKVVFARELGYYELRVDAPEKSLSIREGAQ
                     MVNPVANQ"
     gene            635..1297
                     /locus_tag="AC16QF_04875"
     CDS             635..1297
                     /locus_tag="AC16QF_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6447919.1"
                     /translation="MDFVYSIVEFFATGGAFMYPILLVFAVGAAIAIERFVTLTRVTT
                     KNQTAWSDIQPVLSQGDFERARKMTGQDQSPISRLLSMGLALQGAVRRREDVEIAMEE
                     GMMEIIPQLEKRTPYVALAASIATLLGLLGTIMGLIQAFTAVANANPAEKADLLSASI
                     SVAMNTTAFGLMVAIPLMVVHAFLSTKTNEIVDGMEMASVKVLTAISMRARRGEVTAK
                     AA"
     gene            1339..1860
                     /locus_tag="AC16QF_04880"
     CDS             1339..1860
                     /locus_tag="AC16QF_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR6447920.1"
                     /translation="MARTHHYRRRVTEPTELDVTTFLNLMVVLVPFLLITAVFSRITI
                     VELDLPTSASSPSPNDPTFRVEVIVRDSGLEITNGRMVIAAIPKVEEEYDLQTLGGHM
                     LALKHDHPDIDEASVLLEPEIPYDYLIQVMDVVRSAEIQPEASEGNDGDGEVEQQPTQ
                     LALFTQIAIGDAP"
     gene            1857..>2282
                     /locus_tag="AC16QF_04885"
     CDS             1857..>2282
                     /locus_tag="AC16QF_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR6447921.1"
                     /translation="MKSSRRIKRMDKTRKRSKFVTKMNLTSLMDVFTILVFFLLVNSG
                     AADPLESPKDVKLPESRAESKPKETVVIYISPETVVVQGQPVAMVADILAASGEDVVA
                     IRDRLAAIKGQIIGPNTQQVAGSQEVTIMADKSIPFSVVK"
BASE COUNT          484 a          687 c          706 g          405 t
ORIGIN      
        1 cgacggaacc tcgccgtgct ctgcgacatc tacctggccg acctcgcctg cgctctcgag
       61 cactacgagc gatacagcca ggacattccc gacgatcggg aagtcgagat ctgggtcggc
      121 gacgtccgca accggatgcc cgtgacggac gaggagtgac caagatgaac cgtctcctga
      181 aactgcccct gtatttctgt ctgagcgcgc ttcccgcggc gacctctgtg gcccaggaag
      241 gcgacaaagc catgtcgggc atgtcgatcc tcggcaacaa cgatgctccc aaggcgctgg
      301 tcatcgtgcc ctggaagagc tcggagatag gcgacggcat cgacgttgcc aacctgctcg
      361 acggccgggc aagagcagtg gacaaagtcg tgttcgcaag agagctcggc tactacgagc
      421 ttcgcgtcga cgcgcccgag aaatcgcttt ccattcggga aggcgcccaa atggtgaacc
      481 cggtcgccaa ccagtgaacg gccggcgtcg aaacgtgagg ctcatcaact ggcagaacaa
      541 ccgaacggcg aagggtgcgc cccatcacgt tcacaatcat ctcaacactt taagttgcgt
      601 ctgattcaag gcgttttgcg gagaggttta cgcaatggat ttcgtgtact caatcgttga
      661 attctttgcc actggcggtg ctttcatgta cccgatcctg ctggtctttg cggtaggcgc
      721 ggcgatcgcc atcgagcggt tcgtgacgct gacccgggtg accaccaaga accagacggc
      781 atggagcgac attcagccgg tgctgtccca gggtgacttc gagagggcac gcaagatgac
      841 gggccaggac cagagcccca tctcgcgtct gctgtccatg ggtctggctc ttcagggggc
      901 ggtgcgacgc cgagaggacg tcgagatcgc catggaagag ggcatgatgg aaatcattcc
      961 tcagctggaa aagcgcacgc cctacgtcgc cctagccgcg agcatcgcaa cgctgctcgg
     1021 actgctgggc acgatcatgg gcctgatcca ggcattcacg gcagtggcca acgccaaccc
     1081 ggcggagaaa gccgacctgc tgtctgccag catctcggtc gccatgaaca cgacggcgtt
     1141 cgggctgatg gtcgccatcc ccctgatggt ggtgcacgcc ttcctgtcga ccaagacgaa
     1201 cgagatcgtc gacggcatgg aaatggcgtc ggtcaaagtg ctcaccgcca tctcgatgcg
     1261 cgcgcgtcgc ggcgaggtaa ccgcgaaagc agcctgaagc gacgcattcc aacggggtag
     1321 acggaggcta agggacagat ggccagaacc catcactatc ggcggcgcgt gacggagcct
     1381 acggagctcg acgtgaccac ctttctcaac ctgatggtcg tgctggtgcc gtttctgctc
     1441 atcacggcgg tgttctcgag gatcaccatc gtagagctgg atctgcccac ctcggccagc
     1501 tcgccgtcgc cgaacgatcc gaccttccgg gtggaggtca tcgtgcgtga cagcgggctg
     1561 gagatcacca acgggcggat ggtcattgcg gccataccca aggtggaaga ggagtatgac
     1621 cttcagaccc ttggcgggca catgctcgcg ctgaagcacg atcaccctga catcgacgag
     1681 gcctccgttc tgctggaacc ggaaattccc tacgactatc tgattcaggt gatggacgtg
     1741 gtgcgcagcg ccgagatcca gccggaagct agcgaaggta atgacggtga cggcgaagtc
     1801 gagcagcagc cgacccagct cgcgctgttc acccagatcg ccattggaga cgcgccatga
     1861 agagctcccg acgcatcaag cgtatggaca agacgcgaaa gcgctccaag ttcgtaacga
     1921 agatgaacct gacgtcgctc atggacgtct tcaccatcct ggttttcttc ctgctggtga
     1981 actcgggcgc agccgacccg ctggagtctc cgaaagacgt caagctgccg gagtcgcgcg
     2041 ccgagtccaa gcccaaggag accgtggtca tctacataag cccggaaacc gtggtggtgc
     2101 agggacagcc cgtggccatg gttgccgaca ttcttgcagc atccggcgag gacgtcgttg
     2161 ctatcaggga caggctggcg gccatcaagg gccagatcat cggtccgaac acgcagcagg
     2221 tagcgggcag ccaggaagtg acgatcatgg ccgacaagtc cattccgttc agcgtggtga
     2281 aa
//
LOCUS       DBNHLM010000106         3827 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_973007, whole genome shotgun sequence.
ACCESSION   DBNHLM010000106 DBNHLM010000000
VERSION     DBNHLM010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_973007"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="AC16QF_04890"
     CDS             complement(<1..527)
                     /locus_tag="AC16QF_04890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6447922.1"
                     /translation="MDLDLSEEQNLLIGTVRRFVREEILPLEQSLDPDGDELEPDDHE
                     RLTQLTREMGLYGLDVPPEFGGPDVDLVTRTLLAMEMSQHRAGLYAPCYGVFGGAGLA
                     QLFEATEDQKDRYLFPLLRGEKKGFFGLTEPSGGSDPARAIQTRAVRDGDEWVVNGSK
                     IFISGADRADFGLVFA"
     gene            complement(537..860)
                     /locus_tag="AC16QF_04895"
     CDS             complement(537..860)
                     /locus_tag="AC16QF_04895"
                     /inference="COORDINATES: protein motif:HMM:NF049702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7508 domain-containing protein"
                     /protein_id="HIR6447923.1"
                     /translation="MSIRLTKPWSPASAAGKLPGQMGVYQLGDEAGNVITIGYAGGRS
                     TFGLRGEVETALQRHPDAVSYRVEVNSAYLSRYRELLMVHLADFGTLPPGNEALPGLG
                     RLSPA"
     gene            complement(860..1978)
                     /locus_tag="AC16QF_04900"
     CDS             complement(860..1978)
                     /locus_tag="AC16QF_04900"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6447924.1"
                     /translation="MSYPIISADSHITEHPDTYSAHIDPAWRDRAPKMIDGGERGDLF
                     VIDGMDTPIAMGLVAAAGKPAEELTMAGVRFEELHRSGWDPAARMADQDRDGVAAEII
                     YPTVGMLLCNHRDFDYKKACFDAYNRWIAEYCSTNPARLLGCGQTAVRSPAETVSDLE
                     KIRELGLRGVMMPGDPLEEDYDSQIYNPVWEAAIDLGLPLSFHILTTRQQAPVRGPRI
                     NSFLSIIRGNQDIMGTLIFGGVFDRYPELKVVCVEADAGWVPHYMYRMDHAYKRHRNW
                     LAKGVSLARLPSEYFSEHVYTTFQDDWVAFRLVNDMNWRRLLWANDFPHSDSTWPWSQ
                     QMLAEQTANLTEEQKRGILCDNVAELYRIDVDALAQAA"
     gene            complement(2114..3643)
                     /locus_tag="AC16QF_04905"
     CDS             complement(2114..3643)
                     /locus_tag="AC16QF_04905"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR6447925.1"
                     /translation="MPDRRGRSRILSGACWCLLILCVSGCVYLAQPEDAVVSQAQAPD
                     PQSRIGQLLGGGRGVPARGLLVEASDLALTARIDMIDAADATIDAQYFIWQNDPTGVL
                     VIQKLLDAADRGVRVRALLDDVQLTGFVDRLNALDDHPNIEIRIFNPFSVRFRYPLGF
                     IRIAEFAIDGARLNHRMHNKLMVADNQLAILGGRNIGDDYFGRNPKRNFVDTDILLSG
                     DIVPELSRGFDSYWNSRWAYPVSALMEFSLLPVDLETIRQRIRDRLEEWPDLESLGRE
                     IAFEDTLADLRDAAALDWSTTVVDDPEVGWFERPDDIALDLTELALRAEREVLVVTPY
                     LIPTPNAFKIGKELVDKGVRVRIVTNSLGTNDVVAAQAAYGRYRKRILDTGVELYELR
                     ADAALPERGLSEEVCLHSKYIIIDDEIVFVGSLNLDPRSLYLNTEVGVVLESRPLAAT
                     LRESFDLLAHPDSSWRVTTHENGFRWESSAGTLDRQPAKNGWQRFRYWFLSLIPVSSQ
                     L"
     gene            complement(3709..3813)
                     /locus_tag="AC16QF_04910"
     CDS             complement(3709..3813)
                     /locus_tag="AC16QF_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447926.1"
                     /translation="MGVARNPPVFMDEGDVVEVEVKPVGVLRHRVVVG"
BASE COUNT          679 a         1286 c         1227 g          635 t
ORIGIN      
        1 gcaaacacga ggccgaaatc cgcccggtca gcaccgctga tgaagatctt gctgccattg
       61 acgacccact cgtcgccgtc gcgaacggcc ctggtctgaa tcgctcgtgc gggatcgctg
      121 ccgcccgaag gttcagtgag gccgaagaaa cccttcttct ccccgcgcag cagggggaag
      181 aggtaccgat ccttctgatc ctccgtcgct tcgaacagct gagcgagccc cgcaccgcca
      241 aagaccccgt agcacggcgc gtagagaccg gcgcgatgct ggctcatctc catggcgagc
      301 agcgtccggg tcaccagatc gacgtccggg ccgccgaact ccggcgggac gtccaggccg
      361 tagagcccca tttcgcgggt cagctgcgtc aggcgttcgt gatcatcggg ctcgagctcg
      421 tccccgtccg gatccagaga ctgctccagg ggcaggatct cctcccgaac gaagcgccgc
      481 accgtgccga tcagcaggtt ctgctcctcg ctcagatcga gatccatggc tacccctcaa
      541 gccgggctga gccggccgag ccccgggagg gcctcgttgc cgggcggcag cgtcccgaaa
      601 tccgccaggt gcaccatgag cagctcacga tatcgcgaca ggtaggccga gttgacctca
      661 acgcggtagc tcacggcatc agggtgacgc tgaagagcag tctcgacctc gccgcgcagg
      721 ccgaacgtgc tgcgcccgcc ggcgtagccg atggtgatga cgttgcccgc ctcgtcgccc
      781 agctgataga cgcccatctg gcccgggagc ttcccagcgg cgctggcggg cgaccagggc
      841 ttggtcagcc gtatgctcat caggcggcct gggcgagggc gtccacatcg atgcggtaga
      901 gttccgccac gttgtcgcag aggatccccc gtttctgctc ctcggtcagg ttcgccgtct
      961 gctctgcgag catctgctgg gaccacggcc aggtcgagtc gctgtgggga aaatcgttgg
     1021 cccagagcag tcgccgccag ttcatgtcgt tgaccagcct gaacgccacc cagtcgtcct
     1081 gaaaggtggt gtagacgtgc tcgctgaagt actcggacgg aagcctggcc aggctgacgc
     1141 ccttggccag ccagttgcgg tgacgcttgt aggcgtggtc catccggtac atgtagtggg
     1201 gcacccagcc ggcgtccgcc tccacgcaga ccaccttgag ctccggatag cggtcgaaga
     1261 cgccgccgaa gatgagggtg cccatgatgt cctggtttcc ccggatgatc gaaagaaagc
     1321 tgttgatgcg cggacccctg accggagcct gctggcgggt cgtgaggatg tgaaacgaaa
     1381 gcggcagacc gaggtcgatg gcagcttccc agaccgggtt gtagatctgc gagtcgtagt
     1441 cttcctccag cgggtctccg ggcatcatca cgccgcgcag gccaagctcg cgaatcttct
     1501 ccagatcgga caccgtctcc gccggcgagc gcacggccgt ctggccgcag cccaggaggc
     1561 gggccgggtt cgtcgagcag tattcggcta tccagcggtt gtaagcgtcg aagcaggcct
     1621 tcttgtagtc gaaatcccga tggttgcaca gaagcatgcc aacggtcggg tagatgatct
     1681 ccgcggctac cccgtcccgg tcctgatccg ccatgcgggc tgccggatcc cagccgctgc
     1741 ggtgcagctc ctcgaagcgc acacccgcca tggtgagttc ctccgcaggc tttccagccg
     1801 cagccaccag ccccatggca atcggggtgt ccatcccgtc gatgacgaag aggtcgcccc
     1861 gctcaccgcc gtcgatcatt ttcggagcgc gatcccgcca ggccggatcg atgtgcgccg
     1921 agtaggtgtc cggatgctcg gtgatgtggg aatcggccga gatgatcgga tagctcatcg
     1981 tctctcccct ttgatgacga tcacgccacc gactatcccg gccgacggcg ctgcctgtca
     2041 aggcgccgcg gacggatcag cgggccgccc gcgccccttc gccgcgcgcg gcgaaagccc
     2101 cgaaccccag gcctcagagc tggctggaga ccgggatgag gctcaagaac cagtaacgga
     2161 aacgctgcca gccgttcttt gcaggctggc gatcgagcgt tccggccgac gactcccagc
     2221 ggaatccgtt ctcgtgggtc gtgacccgcc atgagctgtc gggatgggcg agcagatcga
     2281 aggactctcg cagcgtcgcc gccagaggtc gcgactccag caccaccccg acctccgtgt
     2341 tcaggtagag ggacctggga tccaagttca gcgagcccac gaagacgatc tcgtcatcga
     2401 tgatgatgta cttggagtgc aggcagacct cctcggagag cccacgctcc ggcaacgccg
     2461 cgtctgcccg cagctcgtaa agctcgacgc ccgtatccag gatgcgtttg cggtagcgcc
     2521 cgtaggccgc ctgggccgcg accacgtcgt tggttcccag ggagttggtg acgatcctca
     2581 ccctcacccc cttgtcgacg agctcctttc cgatcttgaa ggcgttgggc gtggggatca
     2641 ggtaaggagt aaccaccagc acctcgcgct cggcacgcag cgcgagctcc gtaagatcca
     2701 gggcaatgtc gtccgggcgt tcgaaccagc cgacctcggg gtcgtcgacc accgtggtcg
     2761 accagtccag cgccgcagcg tcgcgcagat cggcaagggt gtcctcgaac gcgatctcgc
     2821 ggccgagcga ttccagatcg ggccactcct cgaggcggtc acggatccgc tgacgtatcg
     2881 tctccagatc caccggcagc agggagaact ccatcagcgc gctcacggga taagcccagc
     2941 ggctgttcca gtaggaatcg aatccccggg acagctccgg aacgatgtcg ccggacaaca
     3001 ggatgtccgt gtcgacgaag ttgcgcttcg ggttcctgcc aaaatagtcg tcgccgatgt
     3061 tgcgaccgcc gaggatggcc agctggttgt cggcgaccat gagcttgttg tgcatccgat
     3121 gattcagccg cgcgccgtcg atggcgaact cggcgatgcg gatgaaaccc agcgggtagc
     3181 gaaagcgcac gctgaaggga ttgaagatcc ggatctcgat gttcggatgg tcgtccagcg
     3241 cgttcagccg gtcgacgaac ccggtgagct gcacgtcgtc tagcagcgcg cgtacccgaa
     3301 ccccccggtc cgccgcgtcc agcagcttct ggattaccag aacgcccgtc gggtcgttct
     3361 gccagatgaa gtactgcgca tcgatggtcg cgtccgccgc gtcgatcatg tcgatgcggg
     3421 cggtcagcgc caggtcggag gcctctacca gcagtccccg agcgggcacc ccgcgcccgc
     3481 cgccgagaag ctgaccgatc cgggattgcg gatcgggcgc ctgggcctga ctgaccacgg
     3541 catcctccgg ctgcgccagg tagacgcacc cggatacgca gaggatgagc aggcaccagc
     3601 aggcgccgga gagaattctc gagcggccgc gacgatcagg cacgggtccg gccagcagtc
     3661 agaatcacgg atggaccgcg gccactcgcc ggtgcggtgg cgaccggctc agcccacaac
     3721 cacccgatgc cgcagcacgc caacgggttt gacctccacc tcgaccacgt cgccctcgtc
     3781 catgaaaacc ggcgggttgc gggcaacacc caccccgccc ggcgtgc
//
LOCUS       DBNHLM010000107         4664 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1332241, whole genome shotgun sequence.
ACCESSION   DBNHLM010000107 DBNHLM010000000
VERSION     DBNHLM010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1332241"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="AC16QF_04915"
     CDS             complement(<1..1106)
                     /locus_tag="AC16QF_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447927.1"
                     /translation="MQSQRVADGSELNRKSIERKGSFADGGLEAASETGDEIVWGALQ
                     MLICALVLVTMLAGSSSWAADPGKEPAGPDPLFASNDLLEITLSGPFQSIARDNDDKQ
                     QQRPGTLTFSDATGEHTVPVRLRARGKSRRVRDVCSFPPIRLNLAKKDVKDTLFEKQD
                     KLKLVAYCKTSASYEQLVLKEYLAYRILNLLTDTSFRVRLMRVNYVDTDRKDKVTTKP
                     SFVIEHEDRVARRLGLEPLSVAKVAPSAHEPAAAALAELYQYLISNTDFSFLAGPVGE
                     ACCHNSVLFSNAAGEVIPIPYDFDVSGLVDAPYAVPPDGLGQRNVRDRKYRGFCRGTE
                     YLDAAVETIQSKRQAIYALVDEVEGFSDRTRRDLS"
     gene            1213..2448
                     /locus_tag="AC16QF_04920"
     CDS             1213..2448
                     /locus_tag="AC16QF_04920"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6447928.1"
                     /translation="MQRSEANDGRWARRAFLRSGALTGTMAGLPFARGAAQAPENGRV
                     RHYAKLGRTGLQISDISFGASRLRRGETDVVRHAVDRGVNYFDTADSYTGGQSEMVIG
                     EALSGMRDRHYIATKTMAGAYDSADRIMQALEGSLKRLKTDYVDVYFNHAVNDVQRLE
                     NDAWFEFAERAKAQGKIRFTGISGHGGRLNECLQHALDEDLVDVILVAHNFGQDPAFY
                     EGLTRSWDLVATQPDLPATLARAKRDDIGVIAMKTLVGARLNDMRPYERDGSTFAQAA
                     FRWTLSNPHVDGLIISMTSTDRIDEFLAASGTGAPGPKDLALLRRYAQLNSVTYCRQA
                     CSACESSCPYDVPIPDVLRMRMYATDYQDVDFARREYAALTTDAAACLSCSGEPCASA
                     CIYGLDVSALCAPTHRMLA"
     gene            2454..3656
                     /gene="larC"
                     /locus_tag="AC16QF_04925"
     CDS             2454..3656
                     /gene="larC"
                     /locus_tag="AC16QF_04925"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HIR6447929.1"
                     /translation="MRTLYYDCFSGISGDMHIGAMVDVGVPVDYLATELDKLCLADEF
                     RLQIEPASKMGIAGTKATVVIDPARPQPPARHLADVRSIIESAGYEEPVATTALGIFQ
                     RIADAEALVHGTTPDKVHFHEVGATDSIVDIVAAAVCIHQLAVDRVMCGPIEVGSGVV
                     RCAHGIMPVPAPATALLLEGVPCHFGRVDGEATTPTGAAILREIVDDFQAPRNFRVAK
                     LGYGIGQKDFSVPNVLRVMLGEEEAAAAAEPYEVESNLEVECNIDDMSAEAFQPLVQD
                     LLDAGAKDVFLTPVIMKKSRPGTKVTVLVGEERFDATLEALFAGSTTIGARVHRVEKR
                     MLPREARRMTTSLGEVRIKLVTRPDGRRTWKVEHDDLVALAERHAMSYLDVRHMIEVE
                     ARRELGDS"
     gene            3653..4405
                     /gene="larB"
                     /locus_tag="AC16QF_04930"
     CDS             3653..4405
                     /gene="larB"
                     /locus_tag="AC16QF_04930"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HIR6447930.1"
                     /translation="MSAQHDELAALLEAYREGTVALDEALSGIRAAFFQDLQHSTIDH
                     DRRRRTGAAEVIYGQHKTPQQIADTFAALAETGGDVLATRVSAEAYELLEARVPHARY
                     HRDAELVMLKQSEPDPAPTKIAVVTAGTSDYRVAEEAALTAEFYDSPVIRISDVGVAG
                     LHRLLSRIDAIRAARVVVVVAGMEGALPSVIGGLVDKPVIAVPTSVGYGAAFDGVAAL
                     LGMLTSCASGVGVVNIDNGFGAGFLANMINRL"
     gene            4416..>4664
                     /locus_tag="AC16QF_04935"
     CDS             4416..>4664
                     /locus_tag="AC16QF_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HIR6447931.1"
                     /translation="MDAKLGAKYEKLKRRIAAFDSAIVAFSGGVDSSLVAYVTAEVLG
                     PRALAVTSGSKSLKRSDLHLAVELADKWGMQHRVIVTDE"
BASE COUNT          906 a         1505 c         1436 g          817 t
ORIGIN      
        1 gacaggtcgc ggcgggtgcg atccgaaaat ccctctacct catcaaccag cgcgtaaatg
       61 gcctgacgct tggactgaat cgtctccacc gcggcatcga gatactcggt tccgcgacag
      121 aatcccctgt acttgcgatc tcttacgtta cgctggccga gcccatccgg cggcacagcg
      181 tacggcgcat cgaccagccc cgacacgtca aaatcataag ggatgggaat gacctctcca
      241 gcggcattgg agaacagcac cgagttgtgg cagcaggctt cgccaacagg tcctgcaaga
      301 aaagaaaagt cggtgttgct gatcagatac tggtacaact ccgcgagcgc ggcagcagcc
      361 ggctcatggg cggatggagc aaccttcgcc accgacaacg gctccaaccc cagacggcgg
      421 gcaacgcggt cttcatgctc gatgacgaag cttggcttgg tcgtcacctt atccttgcga
      481 tcggtatcca cgtagttgac acgcatcagg cgcacccgaa aagacgtatc cgtgagcagg
      541 tttaggattc gatacgccag gtattcctta aggaccaact gctcgtagga ggccgatgtc
      601 ttgcagtagg ccacgagctt gagcttgtcc tgcttctcga acagggtatc tttgacgtct
      661 ttctttgcga ggttgaggcg aatgggcggg aaagaacaca catccctcac acgcctcgac
      721 ttgccacggg cacgcaaacg caccggcacc gtgtgctcac ctgttgcgtc gctgaacgtc
      781 agcgtgcccg gtcgctgctg ctgcttgtcg tcattgtccc gagcgatgga ctgaaacgga
      841 ccggacaacg tgatctctag caggtcgttg ctcgcaaaca gcggatccgg acccgcgggc
      901 tccttaccgg ggtccgcagc ccagctggag gatcccgcca gcatggttac gagcaccaac
      961 gcacagatca acatctgtaa tgccccccaa acaatctcat cgcctgtttc ggacgccgct
     1021 tcgagcccgc catcagcgaa ggagcctttc cgctcaatcg actttctgtt gagctccgat
     1081 ccatctgcta ccctttgaga ctgcatgaat cgttgacaag ggtcaattga acagccgacc
     1141 gtcgcgaaca gtgtctgatc gacaggcgta gcgacctggc gttaacctag cggcagcttt
     1201 acggagaacg acatgcagcg cagcgaagcc aacgatggcc ggtgggcacg aagggccttt
     1261 ctccgatcgg gggccttgac gggaaccatg gccggcctgc catttgcccg aggggcggca
     1321 caagcaccgg aaaacggtcg cgttcgccac tacgcgaaac tcggacgcac cgggttgcag
     1381 atatccgaca tttctttcgg cgccagccgc ctgcgtcgcg gggagaccga cgtggtccgg
     1441 cacgccgtgg accgcggcgt caactacttc gatacggcgg acagctatac gggcggacag
     1501 tccgaaatgg tcatcggcga agccctcagc gggatgcgcg accgtcacta catcgccacc
     1561 aagaccatgg ccggggccta tgactccgct gaccggatca tgcaggcact cgaaggcagc
     1621 ctgaagcgcc tgaagacgga ctacgtcgac gtctacttca atcacgcggt caacgatgtt
     1681 cagcgcctcg agaacgacgc atggttcgag ttcgcagagc gagcaaaagc ccaggggaaa
     1741 atccgcttca ccggcatttc cggacacggg gggaggctca acgagtgcct tcagcatgcc
     1801 ctggacgaag acctggttga cgtgatcctc gtcgcccaca atttcggcca ggacccggcg
     1861 ttctacgaag ggttgacgcg atcgtgggat ctggtggcta cccagcccga cctgcctgcg
     1921 acgcttgctc gagccaagag ggacgacatc ggcgtcatcg cgatgaagac gctcgtgggc
     1981 gcaaggctca atgacatgcg cccctacgag cgggacggat ccactttcgc ccaggccgcc
     2041 ttccgctgga cgctcagcaa tccccacgtg gacggtctca tcatcagcat gacgtccact
     2101 gaccgcatag acgagttcct ggcggcgtca gggaccggag cccccgggcc gaaagacctt
     2161 gccctgctgc gtcgttacgc gcagctaaac agcgttacct actgtcggca ggcgtgcagc
     2221 gcgtgcgaga gctcgtgccc ctacgacgtg ccgatccccg acgtgcttcg gatgcgaatg
     2281 tacgcaaccg actaccagga cgtcgacttc gcgcggcgcg agtacgcggc tctcaccacg
     2341 gacgccgccg cctgcttgtc ctgctcgggt gagccatgcg cgagcgcctg catctatggg
     2401 ctcgacgtca gcgccctgtg tgcgcccacg caccgcatgc tggcctgatt tcaatgagga
     2461 cgctctacta cgactgcttc agcggcatca gcggagatat gcacatcggt gccatggtgg
     2521 atgtgggcgt gccggtcgac tacctggcaa ccgagctcga caagctctgc ctagctgacg
     2581 agtttcgact gcagatcgaa cctgccagca agatgggtat cgcaggaacg aaggctaccg
     2641 tggtcatcga ccccgccaga ccgcaacctc cggcccggca cctcgcagac gttcgctcca
     2701 tcatcgagag cgcaggctac gaggagcccg tggccaccac ggccctcggt atctttcagc
     2761 gaatcgcaga cgccgaggct ctggtccacg gcaccacgcc agacaaggtg cacttccacg
     2821 aggtcggggc gaccgactcc atcgtcgaca tcgtcgctgc ggctgtgtgc attcaccaac
     2881 ttgccgtcga ccgggtgatg tgcggcccca tcgaggtggg cagcggcgtc gttcgctgcg
     2941 cgcacggcat catgcccgtc cctgcgcccg ccaccgcgct tctgctcgaa ggcgtgccct
     3001 gccacttcgg gcgtgtggac ggagaggcta ccaccccgac cggggcagcc atcctcaggg
     3061 agatcgtcga cgacttccaa gcgccacgca attttcgagt cgcaaaactc ggctatggca
     3121 tcggccagaa ggacttctcc gtgcccaacg tcctgcgcgt catgctcggt gaagaggaag
     3181 ccgctgcggc ggccgagccg tacgaggtcg agtcgaatct cgaggtggag tgcaacatag
     3241 acgacatgag cgccgaagcc tttcagccac tcgttcagga cctgctcgac gctggcgcta
     3301 aggacgtctt cctcacgccg gtcatcatga agaagtcccg tcccgggacc aaagtcaccg
     3361 tgctggtcgg cgaggagcgc ttcgacgcca cgttggaagc cctgttcgcc ggctctacca
     3421 ccatcggtgc ccgcgttcac cgcgtcgaga agcggatgct cccccgggag gcccggcgga
     3481 tgaccacctc tctgggcgag gtccgcatca agctcgtcac ccgccccgac ggacgtcgga
     3541 cctggaaagt cgagcacgac gatctggttg ccctggcgga acgccacgcc atgagctacc
     3601 tggacgtcag gcatatgata gaggtcgaag cccggcggga gctcggtgac tcatgagcgc
     3661 gcagcacgac gagcttgcgg ctctgctgga ggcatatcgc gagggcacgg ttgcgctgga
     3721 tgaagcgttg tcgggcattc gcgcggcgtt ctttcaggac ctgcagcaca gcaccatcga
     3781 ccatgaccgt cggcgtagaa ccggcgcggc agaggtcata tacggccagc acaagacccc
     3841 gcagcagatt gccgacacct tcgccgccct ggccgagacg ggcggtgacg ttctcgccac
     3901 ccgggtgagc gccgaggctt acgagctcct cgaggcccga gtgccgcacg cacgctacca
     3961 ccgtgatgcg gagctggtga tgctgaagca gagcgagccc gaccccgcgc ccacgaagat
     4021 cgccgtcgtc accgccggca cgtccgacta ccgggtggcc gaggaagccg cgctgaccgc
     4081 cgagttctac gacagcccgg ttatccgcat cagcgacgtc ggcgtggccg gcctgcacag
     4141 gttgctgagc cggatcgacg ccatccgcgc cgctcgggtg gtcgttgtgg ttgcggggat
     4201 ggagggcgct ctgcccagcg tgatcggagg gctggtggat aaacccgtga tcgccgtgcc
     4261 cacgagcgtc ggttacggcg ccgcgttcga cggggttgcc gccctgctcg gaatgctgac
     4321 gagctgtgca tccggcgtcg gcgtcgttaa catcgacaac ggctttggcg cgggtttcct
     4381 cgccaacatg atcaaccgcc tctgagagac tgcacgtgga cgccaaactt ggtgcgaaat
     4441 acgagaagct gaaacgtcga atcgcggcgt ttgatagcgc gatcgtcgcg ttctcgggcg
     4501 gcgtcgacag ctcgctcgtc gcgtacgtta ccgctgaagt tctggggccc agggcgctgg
     4561 cggtaacttc cggctcaaag agcctgaagc ggagcgacct tcatcttgcc gtcgagctgg
     4621 ccgacaaatg gggtatgcag catcgcgtca tcgtcaccga tgag
//
LOCUS       DBNHLM010000108        16725 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1093146, whole genome shotgun sequence.
ACCESSION   DBNHLM010000108 DBNHLM010000000
VERSION     DBNHLM010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 16725)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16725)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16725
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1093146"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="AC16QF_04940"
     CDS             <1..989
                     /locus_tag="AC16QF_04940"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6447932.1"
                     /translation="AIDGSVGWCTQISSEINALIIRRMEPEFAERIFDDWDALVCSGH
                     GPANGPNPGRLARREGDGWRLNYQGSFASGCHNANWNYLMGAMTVDEATGKPAQASFM
                     VPLGEFEIIDTWHTAGMRGSGSHDVRMADCYVPPENLLPFRSLAPSETWQNPTYRNPT
                     HAIYNKAAVALGVCRGAIDSFIELAMEKTPWGLSSTLKDQAQVQYRIGEAQATLLAAR
                     SFVMETQDKLEEHLGPLPEDGGRLEPEWEYFWPALLACAHAAQSCRKVVDMINNTAGT
                     TGSRMDSPLERQLRDAHQAANHALISYRHYEQLGRTFVGYEPPPGYVELSRA"
     gene            complement(1016..1375)
                     /locus_tag="AC16QF_04945"
     CDS             complement(1016..1375)
                     /locus_tag="AC16QF_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447933.1"
                     /translation="MLLRASGQKENRDYDLTAVTHGERRSGVEQEDWLKRLTEEAIRG
                     EWTALARTLDAASHAIGRETAVDCLVVASAFNGITRVADATGIPLDENTEAATKQMRA
                     AVGLDAFAYAAKSARFD"
     gene            complement(1391..2080)
                     /locus_tag="AC16QF_04950"
     CDS             complement(1391..2080)
                     /locus_tag="AC16QF_04950"
                     /inference="COORDINATES: protein motif:HMM:NF041238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylhydroperoxidase-related (seleno)protein"
                     /protein_id="HIR6447934.1"
                     /translation="MYPDDLPLSEALRNTHQKTLAKIVSPGPHWSGSERRAMAESARA
                     ADDCGLCKTRKTALSPLGVEGHHDGATTLAPTLLDAIHRIRTDPGRLTKRWFEAVAAE
                     IGEGPYVEMLSVVNSLVIVDTLHRCLGQSPPALPDPVAGGPTGEPAGDVVEAGAWVPI
                     ARAPRDMADTGLPVVPNIVRAMGLVPKAVELFFGTFRPHYALKDVKLSLSQAQAEYVA
                     SRVSAMNECFY"
     gene            complement(2202..2969)
                     /locus_tag="AC16QF_04955"
     CDS             complement(2202..2969)
                     /locus_tag="AC16QF_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR6447935.1"
                     /translation="MSARYKPPRRRNLYEPGSAKPFKISRSKIDRFVECPRCFYIDRR
                     LGTDRPPGFPFNINSAVDTLLKTEFDVHRSEGSQHPLQAAYGIDAIPAAHANIDVWRE
                     NFKGVQHLHEPTNLIITGAIDDLWIDGDGSYIVVDYKATAKKEPVTSMEAAWMDGYKR
                     QMEIYQWLLRRNGLDVSDTGYFVYCTGRPDAEAFDAKIDFDVHLIPYQGNDDWVEPTI
                     VALHACLNQPEIPQASWRCDYCAFVGAVNDVTGRSET"
     gene            complement(2979..3977)
                     /locus_tag="AC16QF_04960"
     CDS             complement(2979..3977)
                     /locus_tag="AC16QF_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HIR6447936.1"
                     /translation="MDAVRTPDDRFENLADYPFAPDYVNVDDGQGGALRMHYVDEGEG
                     EVVLCLHGQPSWSYLYRKMIPLLVDAGYRAVAPDLIGFGKSDKPTSRANYTYAAHVHW
                     LNSFVEALDLKQVTVIGQDWGSLIGLRVVAENPDRFARIVLANGALPDAKGIPEAMAP
                     QLKAMLADTPALPIEEMFAKLSGPMEDRPPFMYWVRYCDENPDFHPADVMKGSLAHCS
                     EEEYRAWAAPFPSEEFIQGARQFPSLVPIIPDNPAIPANRAAWSFYEQCDKPFLTAFS
                     DSDPVTKGQEQRFIDSVPGAQGQKHTIIRGGGHFLQDDVGEELAAVVIEFMQDNPL"
     gene            complement(4007..5668)
                     /locus_tag="AC16QF_04965"
     CDS             complement(4007..5668)
                     /locus_tag="AC16QF_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR6447937.1"
                     /translation="MRKLLVLTVVVIFVAVLGWQKRVELVVWGAPKLLALLQPVGENV
                     PVEWAKGPEFPVATPGDRPPNVILILTDDMGFNDISLYNGGAGDGSVMTPNIDALARQ
                     GVTFTNGYAANAVCAPSRASIMTGRYSTRFGFEFTPFPRIGAMLFRWSRDVEESALPS
                     FIDMKAFDALPGDSIADLGMPPSEVTVAEVLQQAGYYTAMVGKWHLGNVGGMQPHEQG
                     FDEALELAGMLYLPEDHPDAVNAKRMDEPIERMVWASARYAARFNGGERFEPDGYLTD
                     YYTAEALQVIESNRHRPFFLYLAHWGIHNPLQATRADYDAYSHIEDHHLRVYSAMIRA
                     VDRSVGLISGKLDELGLADNTLILFTSDNGGASYIQLPEVNKPYRGWKLTHFEGGTHV
                     PFMARWPARIAPGSSFDAPVHHVDLFHTIVAAAGAAIPRDRKLDGVDLVPYVTGQAAG
                     VPHETLFWREGHHQSVQHRGWKLIRSARPDKRWLFDLANDPTEQVDLAGARPDKVEEL
                     VALLDAHNAEQSDPMWPSVLDSPMLVDKHGGQPYEEGDEYIYWPN"
     gene            5779..6819
                     /locus_tag="AC16QF_04970"
     CDS             5779..6819
                     /locus_tag="AC16QF_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6447938.1"
                     /translation="MATREPLLLKGAPGSPYTRKMLAYLRYRHIPYELLIGDQTARLK
                     LPEPKVSLLPTFYLPDEKGELQAVVDSTPLIRRFEIEFPDRQAIPADPVLAFLNYLIE
                     DYADEWLTKAMFHYRWHYDADIEMAGTILPRWTNIQASEAALEKFKAFVSDRQISRLY
                     VVGSNDVTAPVIEASYLRFLEIMNELIERQKFVLGKRPSSADFGIYAQLTQLAKFDPT
                     PARICLDRAPRVYAWTDLVDDLSGHPAEDDGWMDRADASDTLSDLLTEIGRTYAPALL
                     ANAQALRNADEHMEAVIDGQRWEQPTFPYQGKCLQWINAEYRKLDPSARADVDRILQG
                     TGCEAIVLEPDT"
     gene            6823..7866
                     /locus_tag="AC16QF_04975"
     CDS             6823..7866
                     /locus_tag="AC16QF_04975"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6447939.1"
                     /translation="MRKLLYAVLIIVVLLATAAAVVYNTPGGQDALFERAAKAVMGQT
                     PQPLDGMRVVVCGSASPLGNDPNRAQACIAVVTPEHFFLFDVGARSPVRMAQARLPMG
                     RIDGVFLTHFHSDHIAALPDVNLASWVQSRPAALTVYGPPGVGAVVDGFNTAYRLDRG
                     YRTAHHGAELLPPERGPMLAKTFVPGDVVWRDDLMTVTSFEVEHPPIEPAVGYRVDYR
                     GRSVVISGDTNAADTLFTVAKDADLLLHDALSRTLLDPMIATATELGVPVLPTIMTDV
                     IDYHADVTSLPARAAEAGIRQLALYHMVPVPPNALAAKMFLRGIPDDVIVTRDLHTFD
                     LPANSTDIVISEP"
     gene            7870..9156
                     /locus_tag="AC16QF_04980"
     CDS             7870..9156
                     /locus_tag="AC16QF_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR6447940.1"
                     /translation="MKLKTRLRSAAMRQLSSAAYLEKQRAKARKLRQRKGEPPVVHYF
                     HQVDDPYSHLAVQKLDELDDRYDLAFQPHLASKPGEDYRGSAAHFDHWARKDAASVAA
                     DYGTRFAPAINAPAGDAVDAANRVLSAHLDRDDFARAAVSIGEALWSDQRIAEPADAH
                     DGLRAVAEGNDLRRALGHYQGAMFYFEGEWFWGVDRIRLLERRLIDEGFGSSETLCVP
                     EPTPTETSGLDTSSVLLEYFPSLRSPYTAIGHARVLDLIARSGVCVRVRPVMPMMMRG
                     IPAPRAKQRYIITDAGREGRAHGAPFGPIVDPFGDPVKRAFALLPGAAAAGREMEYVT
                     AYLNAAWFEGVDITTQKGLRAVATNAGMDWAALEQASRQENWEAILEENLNTMLKEDL
                     WGVPSFRISGGNSDEAFACWGQDRIWRVENEIASRT"
     gene            9217..9855
                     /locus_tag="AC16QF_04985"
     CDS             9217..9855
                     /locus_tag="AC16QF_04985"
                     /inference="COORDINATES: protein motif:HMM:NF037008.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HIR6447941.1"
                     /translation="MRAAEKLFAERGIENVSIRDIVGEAGQKNESALQYHFKSLTGLI
                     SAIHAERSAETQARRGELLEAMLAQTPDPPLRQLCILMVRPAFDLARGDVGFRRYIKA
                     FGQELALTETSALARAVAHGGGGTSGQQLAGLLKRALPHLDEEAYQRRMEAAVRLCSA
                     SVFHQARQRHAFRGQQADLFFHSLIDALVGLLSAPVSQETRAVAESLGRPEA"
     gene            9900..10598
                     /locus_tag="AC16QF_04990"
     CDS             9900..10598
                     /locus_tag="AC16QF_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447942.1"
                     /translation="MTRIVSRAVLTLVVVTLGAFCFAFFSPRPALTIDPATLAGDGSA
                     IDYCDLPLLDGSGKAAADIPKGNTPGCGYDHFPLPILASCTEQLTEGAADIRGLWVGV
                     EGGFVGHVERVEQCGSRTVVTSSGIIHDSGPNSTAGLNTNDTEGAVLFIIGEKEYCPR
                     TSASMIWNEGVLEFHAFGWGPVVVRRYLEGEQLVWEYLDGSVTRMERICRLPEGQKVP
                     NPRGPRFSIFASKE"
     gene            complement(10629..11006)
                     /locus_tag="AC16QF_04995"
     CDS             complement(10629..11006)
                     /locus_tag="AC16QF_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HIR6447943.1"
                     /translation="MTRWIAVLLMLLWCRPALGGDIGGTWKNDADPVWIEVVHDDGVA
                     TGTVVRNDRNPAAAGRLLLKDLKPGDEANVWRGQVFAVRLGEFRDAQVRLTGDDRMEI
                     LVKVGFMSRTVTWTRADQAVGAE"
     gene            complement(11048..11665)
                     /locus_tag="AC16QF_05000"
     CDS             complement(11048..11665)
                     /locus_tag="AC16QF_05000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6447944.1"
                     /translation="MSEDFSLREAIYNCRAMRRIRPDPVPEELLVELIDAANQAPSGS
                     NSQRARWIVVRDPAVKRQLADQNRRHAEPYIAADVSNPATPKLKRMLDAVVWQMEHMH
                     EIPALIVACFDYPEKVEGMGVYRSAGSVWPGIQNLLLTARALGLGAAPTTLALRDQDA
                     VRKILKLPETFAALCLIPVGYPMGKFGPVTRKPVSEVMRFDAWND"
     gene            complement(11725..12729)
                     /locus_tag="AC16QF_05005"
     CDS             complement(11725..12729)
                     /locus_tag="AC16QF_05005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6447945.1"
                     /translation="MSTSNTRVLLAKRPVGQATPDCFSVETEQLPALEPGQVRVAVEY
                     ISVDAGTRTMLRGEGFHHQVGLGQTILAGGVGHIVDSAADGWELGQAVRGGLGAQTIA
                     TVTPELIERVDESVAPLSAYLGVLGGSTGVTAWIGMRRVAKPQPGDVFVVSAAAGAVG
                     SIAGQIAKRDGARVVGIAGGPRKVSYLLERLGFDAAIDYKNEDVNARLRELAPDGVNV
                     FFDNVGGPILDAVLDNIAMRARVVICGAISQYDDLDNVTGPSLYLRLAERQSTMEGFA
                     FFHFPESIGPATEELARWLADGALVFDEEILEGIERYPEALGFMFEGGNIGKLLVKAR
                     "
     gene            12898..13503
                     /locus_tag="AC16QF_05010"
     CDS             12898..13503
                     /locus_tag="AC16QF_05010"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001886.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HIR6447946.1"
                     /translation="MTYLEWRPHPWHGLEPGDLDSGVVNAFIEITPFDTVKYEVDKES
                     GYLRVDRPSRTSSLPPTLYGFVPRTYCGDRVKVLMEGADSGDFDPLDICVISDRLVNR
                     AEVIIPARVVGGLPMLDGGEADDKIIAVHANDEIWRDTNDIGDLPEQYVARLRHYFAS
                     YKTLPGESSTVHVGEPYGRERALRVLHASISDYQEQFPANP"
     gene            complement(13534..14433)
                     /locus_tag="AC16QF_05015"
     CDS             complement(13534..14433)
                     /locus_tag="AC16QF_05015"
                     /inference="COORDINATES: protein motif:HMM:NF006128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIR6447947.1"
                     /translation="MSQKSYENIKYEVDNGRARISLNRPEKRNALSIELVEELRDALW
                     EADDDTAVHCVILKGEGPSFCAGYDLTAGRSKVDDGVKRRSGASFDDDAWRIERFQRS
                     LRTLAEMHKPSIAQIHGNCLAGGTDLALYCDILICADNANIGYSALRNMGAAPNQMWL
                     YHCGPQWTKRLLLTGDTLSGEDAARIGLVLKSVPEAYLESEVEGLADRLSWIDPEMLS
                     TNKRIINVGMELMGAQVLQRLAAENDARAHTTQAARNVFKQIATEGLRSALRDRDAPF
                     GDSRARVIGPETRDEQGRLVPWE"
     gene            14575..15396
                     /locus_tag="AC16QF_05020"
     CDS             14575..15396
                     /locus_tag="AC16QF_05020"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6447948.1"
                     /translation="MELIVFDLDGTLLNKSAEISDYTRDTLSMLADRGILYTVATGRT
                     LHASVEVLEGHGFRLPQAYKNGVMIWNPTLGDYTHSNFLTLDEIQHVLEAVMRQDITP
                     FMFTLEPGNVHAIYHPPLKTEIEERLAADFRSRSGVGVYPAAQMPPEAEITNISALGI
                     PQAIDAVDLMIADEPHLVAYAGTAIEGSELKWIDIHHVEASKGNAIEILRKELGATKV
                     LCFGDNNNDLSMFEVADESYAPSNAQPEILEAATAVIGHHDEDGIARFLRERFSL"
     gene            15437..16426
                     /locus_tag="AC16QF_05025"
     CDS             15437..16426
                     /locus_tag="AC16QF_05025"
                     /inference="COORDINATES: protein motif:HMM:NF021778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vgb family protein"
                     /protein_id="HIR6447949.1"
                     /translation="MTRSFHGLILVLIAAVSSAIEAAAEPTGTLLVVNKGGDSVTMLD
                     VSDGRELAELPTGRGPHELVVSPDAKLAVVTDYAGGNSLTVIDIAGRRVVRTVDLEDY
                     PRPHGIAFMPDGDTVVVTSEATDQIVFVRISDGAVVGSIETGGAGSHMLAITANGSRV
                     FTSNIQSDTVSELDVASGKQVRAYPTPKQPEAIAVTADGSEIWVGSNAEGSLSVIRTD
                     DATIETIAEGFGWPYRILISRDGRLVLVPDLKRNVLRMFDRTNRVELASVELADAAPQ
                     GLVFHPEERWLFVSLNGQNQVAVIDLSRSAIVRHIDTGPRPDGIGYAATKGQP"
     gene            16423..>16725
                     /locus_tag="AC16QF_05030"
     CDS             16423..>16725
                     /locus_tag="AC16QF_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447950.1"
                     /translation="MSDDCCANGIDASGLETRHRRALASVLAINVAAFLVMIVGAVLS
                     GSSSLLSGTLDNLGDALTYALSLAVIGSTASAKARVAFFKGLLIFAAALGVAAQIGW"
BASE COUNT         3028 a         5521 c         5246 g         2930 t
ORIGIN      
        1 cggccatcga cgggtcggtc ggttggtgca cgcagatatc gtcagagatc aacgcgctga
       61 tcattcgccg catggagccg gagtttgccg aacgaatctt cgacgactgg gatgctctgg
      121 tgtgctccgg tcacggcccc gcgaacgggc ccaatcccgg ccggctggcc cgaagggagg
      181 gcgatgggtg gcggttgaac taccagggct catttgcaag cggctgccac aacgcgaact
      241 ggaactacct gatgggcgcg atgaccgtgg acgaggccac aggcaagccg gcccaagcct
      301 ccttcatggt gcccctgggc gagttcgaga tcatcgatac gtggcacacc gccggcatgc
      361 gcggctccgg tagccacgac gtgcgcatgg ccgactgcta cgtgccgccc gagaatctgc
      421 tgccgttccg gtctctcgca ccgagcgaaa cctggcagaa ccccacctac cggaacccga
      481 cccacgccat ctacaacaag gcggccgtcg ccctcggcgt ctgccgcggc gccatcgact
      541 cgttcatcga gctggccatg gagaaaacgc cctggggctt gagctccacg ctcaaggacc
      601 aggctcaggt ccagtaccgg atcggcgagg cccaggcgac gttgctggcc gcacggtctt
      661 tcgtcatgga aacccaggac aagctggagg aacacctggg gccgcttcct gaagacggcg
      721 ggcgcctgga gccggagtgg gagtacttct ggcccgccct gctcgcgtgc gcccacgccg
      781 cgcagtcctg ccgcaaagtg gtggacatga tcaacaacac cgcgggcacc acgggcagcc
      841 ggatggacag tcccctcgag cggcagctac gggacgccca tcaggcggcc aaccacgcgc
      901 tcatctcgta ccggcactac gaacagctgg gcaggacgtt cgtcggctac gagccgccac
      961 cgggatacgt ggagctttcc cgcgcctgag gcggcgttcg gcgtaggctc gagagctaat
     1021 cgaaccgggc gctcttggcg gcgtaggcga acgcgtccag gccgaccgcc gcccgcatct
     1081 gcttcgtcgc cgcttccgtg ttctcatcca gcgggattcc ggtggcgtcc gccacccgag
     1141 tgatgccgtt gaaggcgctg gccaccacca ggcagtccac ggccgtctcg cgcccgatgg
     1201 cgtggctggc agcgtcgagc gtccgcgcaa gagccgtcca ctcgcctcgg atagcttctt
     1261 cggtcagccg tttcagccag tcctcctgct cgaccccgga tctgcgttca ccgtgggtga
     1321 cggcggtgag atcgtagtcc ctgttctcct tctggccgct cgcccggagc agcatcgtgt
     1381 gggaggtggt tcagtagaag cactcgttca tggccgacac tcgggacgcc acgtactccg
     1441 cctgtgcctg agagagagaa agcttgacgt ccttcagagc gtaatgcggc cggaacgtgc
     1501 caaagaagag ctctaccgct ttgggcacca gccccatggc tcggacgatg ttcggcacca
     1561 cgggcaggcc cgtatccgcc atgtccctcg gcgcgcgggc gatcggtacc caggcgccgg
     1621 cttccacaac gtccccggcg ggctcccccg tgggcccacc ggccaccggg tccggcaaag
     1681 cgggcggtga ttgaccaaga caccggtgca gtgtgtcgac gatcaccagg ctattgacca
     1741 cgctgagcat ctccacgtag ggaccctcgc cgatctccgc cgcgaccgcc tcgaaccagc
     1801 gtttggtcag ccgcccggga tccgtgcgga tacgatggat ggcatccagg agcgtcggcg
     1861 cgagagtcgt ggcgccatcg tggtggcctt cgacccccaa aggagagagg gccgtcttgc
     1921 gcgtcttgca caacccacag tcgtcggcgg ctctggcgga ttccgccatg gcacgccgtt
     1981 cactgcccga ccagtgaggg ccggggctga cgatcttggc gagcgtcttc tggtgcgtgt
     2041 ttctcagcgc ttcgctcagc ggcaggtcgt ctggatacat ggcatcactc tcccccggat
     2101 tcgagacctt ccgacaattg gaacggccgg ccgcagacag gccagagcgt aacggctcgg
     2161 caaggagacc accagctgag cgaagcgggc cggtcgtcgc ctcaagtctc tgaccgcccg
     2221 gtgacgtcgt tgacggcacc gacgaaagcg cagtaatcgc accgccagct cgcttgcggt
     2281 atctccggct ggttcaggca ggcgtgcagc gccacgatgg tgggctccac ccaatcgtcg
     2341 ttgccctggt agggaatcag gtgaacgtcg aagtcgatct ttgcatcaaa cgcctctgcg
     2401 tccgggcggc cggtgcagta gacgaagtag ccggtatcgg acacatcgag cccgttacgc
     2461 cgcaggagcc actggtagat ctccatctga cgcttgtagc catccatcca ggcggcttcc
     2521 atggaggtga cgggctcttt cttggccgtc gccttgtagt ccaccacgat gtaggagcca
     2581 tcgccgtcaa tccacagatc gtctatggcg ccggtgatga tcaggttggt gggctcgtgg
     2641 aggtgctgaa cccccttgaa gttctcgcgc cagacgtcga tgttggcgtg cgcggcggga
     2701 atcgcgtcga taccgtaggc cgcctgcagc ggatgctgac taccctctga ccggtgaacg
     2761 tcgaactcgg ttttcagcag cgtgtccacg gcgctgttga tgttgaaagg gaagccggga
     2821 ggacggtcgg tgcccaggcg cctgtcgatg taaaagcaac gcgggcactc aacgaaacgg
     2881 tcgatcttcg accgggagat cttgaacggc ttcgcgcttc ccggctcgta gagattgcgg
     2941 cgtcgcggcg gcttgtatct cgcgctcatc gcaacgggtc agagcgggtt gtcttgcatg
     3001 aactcgatca ccaccgcggc aagctcctcg ccaacgtcgt cctgcaggaa gtggccaccg
     3061 cctctgatga tcgtgtgctt ctggccctgc gcgcccggca cggagtcgat gaagcgttgc
     3121 tcctggcctt tggtcacggg atccgaatcg ctgaaggcgg tcaggaacgg tttgtcgcac
     3181 tgctcgtaga acgaccaggc cgcacggttg gcgggaatcg ccgggttgtc gggaatgatg
     3241 ggcacgagcg acgggaactg ccgtgcgccc tgtatgaact cttccgaggg gaacggcgca
     3301 gcccaggccc ggtattcctc ctccgagcag tgggccagcg aacccttcat gacgtcagcc
     3361 gggtgaaagt ccggattctc gtcgcagtat ctcacccagt acatgaacgg cgggcggtct
     3421 tccatgggcc cggagagctt cgcaaacatc tcctcgatcg gcagcgcggg cgtgtcggcc
     3481 agcatggcct tcaactgagg cgccatggcc tccgggatcc ccttggcgtc gggcagcgcg
     3541 ccgttcgcca gcacgatgcg ggcaaaacgg tcggggtttt cagccacgac ccgcaacccg
     3601 atgaggctgc cccagtcctg gccgatgacc gtgacctgct tcaggtccag ggcctcgacg
     3661 aagctattca accagtgcac gtgcgctgcg taggtgtagt tcgcgcggct cgtgggcttg
     3721 tcggatttgc cgaagccgat cagatcgggc gcaacggccc gatatccggc atcgacgagc
     3781 agcgggatca tcttgcggta cagataggac cagctcggct ggccatgcag gcacaggacg
     3841 acctctcctt caccttcgtc aacgtagtgc atgcgcaacg ccccgccttg gccgtcgtcg
     3901 acgttcacgt agtcaggggc aaaggggtag tcggcgagat tttcaaatcg atcgtctggg
     3961 gttctgacgg cgtccatcgt tcgtattcct gatctgtttg cgggtttcag ttgggccagt
     4021 agatgtactc gtcgccttcc tcgtaaggct ggccgccatg cttgtcgacg agcatcggcg
     4081 aatccagcac cgatggccac atggggtcgg actgctcggc attgtgggca tccagcagcg
     4141 ccacgagctc ctcgaccttg tccggccgtg ccccggccag atcgacctgc tccgtaggat
     4201 cgttggccag atcgaacagc cagcgcttgt cgggccgggc cgagcggatc agcttccagc
     4261 cccgatgctg aaccgactgg tggtgccctt cccgccagaa cagggtttcg tggggcacgc
     4321 cggctgcctg accggtgacg tagggaacga ggtccacgcc gtcgagcttt cggtccctcg
     4381 gaatggccgc gcccgccgcg gcgacgatgg tgtggaacag gtccacgtga tggacgggcg
     4441 catcgaaact cgatcccggc gcgatgcgag ccggccatcg agccatgaag ggcacgtggg
     4501 tgcccccttc gaaatgggtc agcttccagc cgcgataggg cttgttgact tcgggcagct
     4561 gtatgtagga agcaccgccg ttgtcgctgg tgaacaggat cagcgtgtta tcggcaagcc
     4621 cgagctcgtc cagctttccg gatatcagac ccacgctgcg gtccacggcc cggatcatcg
     4681 ccgagtagac gcgcagatga tggtcttcga tgtgcgagta cgcatcgtag tctgctctgg
     4741 tcgcctgcag cgggttgtgg attccccagt gggcgagata gagaaagaac ggtcggtgtc
     4801 ggttgctctc gatgacctgg agtgcttcgg cagtatagta gtcggtcagg tacccgtcgg
     4861 gctcgaagcg ctcgccgcca ttgaagcgcg cggcatatct cgcactcgcc cagaccatcc
     4921 gctctatcgg ctcgtccata cgcttggcgt ttaccgcatc gggatgatct tccggcaggt
     4981 aaagcatccc ggcaagctcc agagcctcgt caaagccctg ctcgtggggc tgcatgccgc
     5041 cgacgttgcc caggtgccac ttgcccacca tggccgtgta gtagccggcc tgctgcagca
     5101 cctcggcgac ggtgacctcg ctgggcggca tgcccaggtc cgcgatgctg tccccgggca
     5161 gcgcatcgaa agccttcatg tcgatgaagc tcggcaatgc ggactcctcg acatcccggc
     5221 tccacctgaa aagcatggcg ccgatccgcg ggaacggcgt gaactcgaat ccgaagcggg
     5281 tcgagtaccg gccggtcatg atgctggccc gggacggggc acagaccgcg ttcgccgcgt
     5341 agccgttggt gaaggtgact ccctggcggg caagcgcgtc gatgttcggt gtcattaccg
     5401 agccgtctcc ggcaccgccg ttgtacagcg agatgtcgtt gaagcccatg tcgtcggtca
     5461 ggatgagaat cacgttcggg ggccgatcgc cgggcgtcgc gacggggaac tcggggcctt
     5521 tggcccactc tacgggaacg ttctccccca ccggctgcag cagcgcgagc agcttgggcg
     5581 cgccccagac aaccagctct acccgcttct gccagccaag cacggccaca aagatcacca
     5641 cgaccgtcag aacaaggagc tttctcatcg tgtcaggacc accgggcatc ggacgtggga
     5701 acgaatgata cacaccaaat aatgtttgtc attatatttg tgaggcgcta tcctgactca
     5761 tcttcagaga gaagactcat ggccacacgc gaaccgctcc tgctcaaagg ggcacccggc
     5821 tccccctaca cccgcaagat gctggcttac ctgcgctatc ggcatatccc ctacgaactg
     5881 ctcattggcg atcagacggc ccggctgaag ctgcccgagc cgaaggtgag cctgctgccg
     5941 accttctatc tgccggacga gaagggcgag ctccaggcag tggtggactc cacgccgctc
     6001 atccgccgct tcgagatcga atttcccgat cgccaggcga tacccgcgga tccggtgctg
     6061 gcctttctga actatctcat cgaggactat gcggacgagt ggctcaccaa agccatgttc
     6121 cactaccgct ggcactacga tgcggacatc gagatggcgg gaaccatcct tccccgctgg
     6181 accaacattc aggcttcgga ggcggcgctc gagaagttca aggcgttcgt ctccgatcgg
     6241 cagatctcga gactttacgt ggtcggctcg aacgacgtca ccgccccggt gatcgaagca
     6301 agctacctgc gattcctgga gatcatgaac gagctgatcg agcggcagaa gttcgtgctc
     6361 ggcaaacgtc cttcctccgc ggatttcggg atctacgccc agctcacgca gctggccaag
     6421 ttcgacccga cgcccgctcg aatctgcctg gatcgggcgc ccagagtcta tgcctggacg
     6481 gatctcgtcg acgatctcag cggccacccg gcggaagacg acgggtggat ggatcgcgca
     6541 gacgcctcgg atacgctctc agacctgctg accgagatcg gtcgcaccta cgcgccggcc
     6601 ctgctggcga acgcccaggc gctgcgcaac gcagacgagc acatggaagc cgtcatcgac
     6661 gggcaacgct gggagcagcc gaccttcccc tatcagggca agtgcctgca atggatcaat
     6721 gccgagtacc ggaaactcga tccgtcggca cgagccgacg tcgaccgcat actgcagggc
     6781 accggctgcg aagccatcgt cctcgaaccg gatacgtaga tcatgagaaa actactgtac
     6841 gcagttctga tcatcgtcgt gctcctcgcc acggcggccg ccgtcgtcta caacaccccc
     6901 ggcggccagg acgcgttgtt cgagcgcgcc gccaaggccg ttatgggcca gaccccacag
     6961 cctctggacg gcatgcgcgt ggtcgtgtgc ggcagcgcgt cgccgctcgg caacgatccg
     7021 aatcgggctc aggcatgcat tgccgtcgtt accccggagc actttttcct gttcgacgtg
     7081 ggcgcgcgct cgccggtcag gatggctcag gcgcgattgc ccatgggccg gatcgacggg
     7141 gtgtttctga cgcacttcca ttccgatcac atcgccgcgc tgcccgacgt gaacctggcc
     7201 tcctgggtgc agagcagacc ggccgcgctg acggtgtacg gcccgccggg agtcggggcg
     7261 gtggtggacg gattcaatac cgcttaccgg ctagaccgcg gctaccgcac ggctcaccac
     7321 ggcgccgagc tgttgccgcc cgaacgcggc ccgatgctgg cgaaaacgtt cgtccccggc
     7381 gacgtcgtct ggcgcgacga cctgatgacg gtgacctcgt tcgaagtgga gcatccgccc
     7441 atcgagcccg cggtgggcta ccgcgtcgac tatcggggac gctcggtggt gatctccgga
     7501 gataccaatg cggcggacac cctgttcacc gttgccaaag acgcagacct gctgctgcac
     7561 gacgcgctgt cgcgtacgct gctggacccg atgattgcca ccgccaccga gttgggcgtt
     7621 ccggtccttc cgaccatcat gaccgacgtc atcgactatc acgccgacgt caccagcctg
     7681 cctgcgcgcg cggcggaagc cggcatcagg cagctggccc tgtaccacat ggtgcccgtc
     7741 ccacccaacg cgctggcggc aaagatgttc ctcaggggca ttccggacga cgtcatcgtc
     7801 acgcgggatc tgcatacctt cgatttgccc gcgaactcca cggacatcgt cattagcgag
     7861 ccttgagcca tgaaactcaa aacccgactg cgctcggctg ccatgcgcca gctctcctcg
     7921 gccgcctatc tggagaaaca gagggcaaag gcgcgaaagc tgcggcagag gaagggcgag
     7981 ccgccggtcg tgcactactt ccaccaggtg gacgatccct acagtcacct ggcggtgcag
     8041 aagctcgacg agctagacga ccgctacgat ctcgcttttc aaccccacct ggcttccaag
     8101 ccaggcgaag actaccgggg cagcgccgct cacttcgatc actgggcacg caaggacgcc
     8161 gcaagcgtgg ccgcggatta cggcacccgg ttcgcgcccg ccatcaacgc gcccgccggc
     8221 gatgccgtgg atgcggcaaa ccgggtgctg tcagctcacc tggatcgcga cgatttcgcc
     8281 cgcgctgccg tcagcattgg cgaggcgctg tggagcgatc agagaatcgc cgagccggcc
     8341 gacgctcacg acggcctccg ggccgttgcc gaaggaaacg acctgcgccg cgcgctcgga
     8401 cattaccagg gagccatgtt ctacttcgaa ggggaatggt tctggggtgt cgaccgaatt
     8461 cgtctgctgg agcgaaggct gatcgacgag ggcttcggca gcagcgagac cctttgcgtg
     8521 cccgagccga ccccaacgga aaccagcggc ctcgacacca gttctgtgct gctggagtac
     8581 ttcccgtctc tgcgcagccc ctacacggcc atcggtcacg cccgcgtgct cgacctcatc
     8641 gcacgcagcg gggtttgcgt tcgagtgcgg ccggtgatgc cgatgatgat gcggggcatt
     8701 cccgcgccgc gcgcgaagca gcgctacatc atcacggacg ccggccgcga agggagagca
     8761 catggcgcgc cgttcggacc catcgtcgat ccgttcgggg atccggtgaa gcgtgccttt
     8821 gctctgctcc ccggtgcggc cgcagccggc cgggagatgg agtacgtcac cgcatacctc
     8881 aacgccgcct ggttcgaggg cgtggacatc acaacgcaga aaggcctgag agcggtggcg
     8941 acaaacgcgg gaatggattg ggccgcgctc gagcaggcca gccggcagga gaactgggaa
     9001 gccatactgg aggaaaacct caacacgatg ctcaaagaag acctttgggg cgtgccgagt
     9061 tttcgaatct cgggcggaaa cagtgacgag gccttcgcgt gctggggaca ggaccgcatc
     9121 tggcgggtcg agaacgaaat cgcgagccga acgtagatgc cggcgcggag ccaagcgggt
     9181 agacactcga gaagcagagc cacccaggac gcgctgatgc gcgccgcgga aaaactgttc
     9241 gcggaacggg gcatcgagaa cgtgtcgatt cgcgacatcg tgggcgaagc gggtcagaag
     9301 aacgagtccg cgctgcagta ccatttcaag agcctcaccg gcctcatctc tgccattcac
     9361 gccgagcgat cggcggaaac ccaggcccgg cgcggagagc tgctcgaagc gatgctggct
     9421 cagacacccg accccccgct acgccagctc tgcattctga tggtcaggcc tgcattcgac
     9481 ttggcccgcg gtgacgtggg tttccgtcgc tacatcaaag cgttcgggca ggagctggct
     9541 ctcacggaga cctcggctct ggctagagcg gtcgcccatg gcggaggtgg aaccagcgga
     9601 cagcagctgg ccgggctgct gaaacgggcg ctgcctcacc tggatgagga ggcctatcaa
     9661 cgccggatgg aggcagcggt gcgcctgtgt tccgcttccg tgttccatca ggccagacag
     9721 cggcacgcct ttcgcggcca acaggcagat ctgttcttcc acagcctcat cgacgcgttg
     9781 gtggggctac tgagcgcgcc cgtttcccag gagacccggg cagtcgccga atccctcggg
     9841 cggcccgagg cgtgaggttt gggtcgcgca atgcagcggc ccggcgctga caggctgtcg
     9901 tgacgaggat cgtctcccgc gctgtcctta ccctcgtcgt cgttaccctg ggcgcgttct
     9961 gcttcgcgtt cttctccccc cgcccagccc tgaccatcga cccggcaacc ctggccgggg
    10021 acggcagcgc catcgactac tgcgatctgc cgctcctcga cggcagcggg aaggcagccg
    10081 ccgacattcc caaaggcaac acgcccggct gcggctacga tcactttccc ctgccgatac
    10141 tggcaagctg taccgagcaa ctgacggagg gtgcagccga cattcgaggc ctgtgggtcg
    10201 gggtggaagg cgggttcgtc gggcacgtgg aacgcgtgga gcagtgcggc tcccgcacgg
    10261 tcgtgaccag cagcggcatc attcacgact ccggaccgaa cagcacggca ggcctgaaca
    10321 ccaacgacac cgagggcgcc gtgctcttca tcatcggtga aaaggaatac tgcccgcgca
    10381 cctcggccag catgatctgg aacgaggggg tactcgagtt ccacgccttc ggctggggac
    10441 ccgtcgtggt gcgtcgctac ctggagggcg agcagctggt ctgggaatac ctggacggca
    10501 gcgtgaccag aatggaaaga atctgccgat taccggaagg ccagaaagtg ccgaacccgc
    10561 gaggcccgcg gttctctatc ttcgccagca aggagtaatg ggcccggcga cccgccccca
    10621 aaggctactt actccgcgcc cacggcctgg tcggcccgcg tccaggtcac tgtacggctc
    10681 atgaaaccga ccttgaccag gatttccatg cggtcatcgc cggtcaagcg cacctgggcg
    10741 tccctgaact cgcccagccg caccgcgaag acctgacccc gccagacgtt cgcttcgtcg
    10801 cccggcttca gatctttgag cagcagccgg ccggccgccg cgggattgcg gtcgttgcgg
    10861 accacggtgc cggtggccac gccgtcgtca tgaacgacct ctatccacac gggatcggcg
    10921 tcgtttttcc aggttccgcc gatgtcacca ccgagtgccg gccggcacca cagcagcatc
    10981 agcagaacag caatccatct cgtcacgggt cggtttctcc tccttccggc gggcaaaata
    11041 ccggccgcta gtcgttccag gcgtcgaacc gcatgacctc agaaacgggc tttcgggtga
    11101 cgggcccgaa cttccccatc ggatagccga ccgggatcag gcagagcgcg gcaaaggtct
    11161 caggcagctt cagaattttc cgaacggcat cctgatcgcg gagcgcgagg gtggtgggcg
    11221 ccgcgccaag gcccagagcg cgagctgtca gcagcaggtt ctggatgcca ggccatacgg
    11281 acccggcgct gcggtacacg cccatgccct ccaccttctc ggggtaatcg aaacaggcga
    11341 cgatcagcgc cggtatctcg tgcatgtgct ccatctgcca tacgacggca tcgagcatcc
    11401 tcttcagctt cggcgtggcc gggttagaga cgtcggcagc aatgtaaggc tctgcgtgcc
    11461 tgcggttctg gtccgccagc tgacgcttga ccgcggggtc tctgacgacg atccatcgcg
    11521 ccctctggga gttcgatccc gacggcgcct ggttcgccgc atcgatcagc tcgacgagga
    11581 gttcctccgg aacggggtcg ggcctgatcc ggcgcatggc ccggcagttg tagatcgcct
    11641 cgcgaagtga gaagtcttca gacaccttgg cttcctgtga gtttacgaat caaaagaggg
    11701 tgcaaagtgc ggcgaccccg ttggtcaacg cgccttcacg agcagcttgc ctatgttgcc
    11761 gccttcgaac atgaacccaa gcgcctcggg gtagcgctct ataccttcca ggatctcctc
    11821 gtcgaaaacg agcgcgccgt cggccagcca ccgcgcaagc tcctcggtcg ccggtccaat
    11881 gctctcaggg aagtgaaaga acgcaaaccc ttccatggtg gactggcgct cggccagccg
    11941 aaggtagagc gaggggccgg tgacgttgtc cagatcgtcg tattgcgaga tggctccaca
    12001 gatgacgacc ctcgcccgca tcgcgatgtt gtccagcacg gcatccagga tggggccgcc
    12061 cacgttgtcg aagaagacgt tcaccccgtc gggcgccagc tcgcgcaggc gggcattgac
    12121 gtcctcgttc ttgtagtcga tcgcagcgtc gaaccctagg cgttcgagaa ggtaggagac
    12181 ctttcgggga ccgccggcga ttccgacgac gcgcgcgccg tcgcgtttgg caatctgacc
    12241 ggcaatggat cccaccgctc ctgccgccgc ggacaccacg aaaacgtccc ccggctgagg
    12301 tttggcgacg cgccgcatgc caatccaggc ggtcacgccc gtggatcccc ccagcacgcc
    12361 cagatacgcg ctcaggggtg ctacggattc gtcaacgcgc tcgatcaatt ccggcgtgac
    12421 cgtcgcgatc gtctgcgcgc caagcccgcc gcgaactgcc tgacccaatt cccagccatc
    12481 tgcggccgag tcgacgatgt ggccaacccc gcccgcgagt atcgtctgcc cgagtcccac
    12541 ctgatgatga aacccctctc cgcgcagcat ggtgcgcgtt ccggcatcca ccgagatgta
    12601 ctcaacggcg acgcgcacct ggccaggctc gagggccggc agctgctcgg tctccacgga
    12661 gaaacagtca ggcgttgcct gtccgacggg ccgctttgcc agcaggacac gggtgttcga
    12721 agtagacatc gcgcgagcct agcacagccc gcccaccttc gccccgtccc gcatcccgcc
    12781 gtccctagaa gccgtcttaa gccgcgaaaa agcagccatc gaaaggtgac gccaaatctg
    12841 ccaattaccg gcgggcacag atatagtcca ccttcaccaa ccaacgcaga acgaccagtg
    12901 acctacctag agtggcgccc ccacccgtgg cacgggctcg agcccggcga cctcgactcc
    12961 ggtgtcgtca acgccttcat cgagatcacg cctttcgaca cggtgaaata cgaggtcgac
    13021 aaggagtcgg gttatctgcg ggtggaccgg ccgagccgca cctcctcgct gccaccgacg
    13081 ctgtacggtt tcgtaccgcg cacgtattgc ggagaccgtg tgaaagtgct gatggaaggg
    13141 gcggactcgg gagatttcga ccctctggac atctgcgtga tcagtgaccg gctggtcaac
    13201 cgcgccgagg tcatcatccc ggcacgggtg gttggcgggc taccgatgct cgatggcggg
    13261 gaggccgacg acaagatcat cgcggttcat gcgaacgacg agatctggcg cgacaccaac
    13321 gacattggcg acctgcccga gcaatacgtt gcccgcctgc ggcactactt cgcctcgtac
    13381 aagacccttc ccggggaatc gtcgaccgtc cacgtgggcg agccgtacgg gcgcgagcgg
    13441 gcgctgcggg ttctgcatgc gtcgatttcc gactaccagg aacagtttcc cgcaaacccc
    13501 tgagcccacc gggtgcggct gcgggccctc tgatcactcc cagggcacca gccttccctg
    13561 ctcgtccctg gtttccggcc cgatgaccct ggcacggctg tcgccgaacg gcgcatcccg
    13621 gtcccgaagc gcggagcgca gcccttcggt ggctatctgc ttgaacacgt ttcgcgccgc
    13681 ctgcgtggtg tgagcccggg catcgttctc cgccgccagc cgctgcagca cctgggcacc
    13741 catgagctcc atgcccacat tgatgatccg cttgttggtg gagagcattt ccggatcgat
    13801 ccagctcaac cggtccgcaa gcccttccac ttcgctctcc aggtacgctt cgggcacgga
    13861 cttcagcacg agccctattc tggccgcatc ctcgccgctc agcgtgtccc ctgtcagcag
    13921 aagacgcttg gtccattgcg ggccgcagtg atacagccac atctggttcg gcgccgcgcc
    13981 catgttgcgc agggccgagt aaccaatgtt cgcgttgtcg gcgcagatca gaatatcgca
    14041 gtacagcgcg aggtccgtgc cgcctgccag acagtttccg tgtatctggg caatagacgg
    14101 tttgtgcatc tcggcaagcg ttcgcagact gcgctgaaac cgttctatcc tccacgcatc
    14161 gtcgtcgaag ctggcgccag aacggcgctt gaccccgtca tccaccttcg accgaccggc
    14221 tgtcaggtcg tagcccgcgc agaacgaggg gccctcccct ttcagaatga cgcagtgaac
    14281 ggccgtatcg tcgtcggctt cccacaacgc gtctctgagc tcctcgacca gctcgatgga
    14341 cagcgcgttg cgcttctccg gccgattcag agagatccgg gctcgtccgt tgtcgacctc
    14401 gtacttgatg ttctcgtatg acttctggct catgggatct cctgtggatg cgcggtagcg
    14461 gcagttccgg atattgcctg cgccccacgg gtagcagcaa ccgttggaac ctcccggagc
    14521 acggcgaatc ccgaaaggtt tgacccagcc gctcagtctg caacactgcg ccggatggaa
    14581 ctgatcgttt tcgacctcga cggcaccctg ctgaataaat ccgcggagat ctccgattac
    14641 acccgcgaca cgctttcgat gctggcggac cgcggcatcc tgtacacggt cgccaccggc
    14701 cgaacgctgc acgcgtccgt cgaggtcctc gagggccacg gcttccgcct gccccaggcg
    14761 tacaagaacg gtgtgatgat ctggaacccg acgcttgggg actacaccca cagcaacttt
    14821 ctgaccctcg acgagatcca gcacgttctg gaggcggtga tgcgccagga catcacgccc
    14881 ttcatgttca cgctggagcc cggcaacgtc catgcgatct accatccgcc cctgaagacc
    14941 gagatcgagg aacgcctggc cgccgatttc cgttcacggt ccggcgtggg cgtgtatccc
    15001 gccgctcaga tgccccccga agccgagatc accaacatca gcgcgctcgg gataccccag
    15061 gccatcgacg ccgtcgatct gatgattgcc gacgagccgc atctggtggc gtatgcgggt
    15121 acggcaatcg aaggctcgga gctgaagtgg atcgacattc accacgtgga ggcgagcaag
    15181 ggcaacgcca tcgagatcct gcgcaaggag ctcggcgcga ccaaggtgtt gtgcttcggc
    15241 gacaacaaca acgacctcag catgttcgag gtcgcagacg aaagctacgc gcccagcaac
    15301 gcccagcccg agattctgga ggcggcgacc gccgtgatcg gccatcacga cgaggacggc
    15361 atcgcgcgat ttctccgcga gcggttctcg ctgtagcaga ggacggacgc cgcaccccga
    15421 tgagcaggga gacaggatga ccagaagttt ccacggcctg atcctcgtgc tcatcgccgc
    15481 ggtgtcttca gccatcgagg cggcggccga gccgacggga acgctcctcg tcgtcaacaa
    15541 aggaggcgac agcgtaacga tgctggatgt gtccgatggc cgcgagctcg cggagcttcc
    15601 cacaggccgg ggaccgcacg agctcgtggt ttcgccggac gcgaagctgg ctgtggtgac
    15661 ggactacgcc ggcggcaact cgctcaccgt catcgacatc gctgggcgac gcgtcgtccg
    15721 cacggtggat ctcgaggact acccgcggcc ccacggtatc gccttcatgc cggacggtga
    15781 cacggtggtc gtgacgtccg aagccaccga tcagatcgtg ttcgtgcgca tttctgacgg
    15841 ggccgtggtg ggatccatcg aaaccggcgg cgccggatcc cacatgctcg ccatcaccgc
    15901 gaacggatcg cgcgtcttca cgagcaacat ccagagcgac acggtctccg agctcgacgt
    15961 ggcctccggg aaacaggtgc gcgcctaccc aaccccgaaa caacccgaag ccatcgcagt
    16021 gaccgctgac ggctcggaaa tctgggtcgg cagcaacgct gaaggctcgc tgagcgtgat
    16081 ccgaaccgat gacgccacca tcgagacgat tgccgaaggc ttcggttggc cgtaccgtat
    16141 cctgatctcc agggacggca ggctggtgct ggtcccggac ctcaagagga acgtgctgcg
    16201 catgttcgat cgcaccaatc gggtagagct agccagcgtg gagttggcgg acgcggcgcc
    16261 ccagggactc gtcttccatc cggaagaacg ctggcttttc gtctcgctca atggtcagaa
    16321 tcaggtggcc gtcatcgatc tgagcagatc cgccatagtc cggcacattg acacgggccc
    16381 acggcctgac ggcattggct acgccgcgac gaaggggcag ccatgagcga cgattgctgc
    16441 gccaacggaa tcgacgcgag cggtctcgaa acgcggcacc ggcgcgcgct ggcgtccgtt
    16501 ctcgccataa acgtcgccgc attcctcgtg atgatcgtgg gcgccgtgct cagcggctca
    16561 tcctctctgc tctccggcac cctggacaac ctcggcgacg cgctgaccta cgcccttagc
    16621 ctggcagtca tcggttccac ggcatccgca aaggcccggg tcgccttttt caagggcttg
    16681 ctgatctttg cagccgccct cggggtggcc gcgcagatcg gctgg
//
LOCUS       DBNHLM010000109         7135 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1392478, whole genome shotgun sequence.
ACCESSION   DBNHLM010000109 DBNHLM010000000
VERSION     DBNHLM010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1392478"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="AC16QF_05035"
     CDS             complement(<1..381)
                     /locus_tag="AC16QF_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447951.1"
                     /translation="MGSDFDHLHGDFNIEDSAFAEQFEDVLEHLVSKCPVARSQVGPG
                     YRVFNRYRDVRQIGQDWRTFSSADGWMLDPPEGNIPILPEDCDPPYHTAWRQALNPFF
                     NAKEVAKLEDHARGYARELIDGFAD"
     gene            595..2109
                     /locus_tag="AC16QF_05040"
     CDS             595..2109
                     /locus_tag="AC16QF_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014756484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6447952.1"
                     /translation="MPIKRPSHGELRSVAQRLNLTLSENDVETFRDLMGASIDAFNQV
                     EAMDDELPPVRYPRDSGTRPSAADNPLNAWYYRTSIQGADGGALAGRTVALKDNIMLA
                     GVPMMCGSATLEGYVPEIDATVVERILDAGGEIAGKAHCEFFCLSGGSHTNSTGPVHN
                     PHKRGYSAGGSSSGSAALVAAGDVDMAIGGDQGGSIRIPASFCGVYGMKPTHGLVPYT
                     GVMPLEVFVDHIGPMTANVEDNATLLEAIAGPDAYDPRQRGVETHRYTEALGQDIAGL
                     KIAVLKEGFGHPNSESDVDDCVRAAAAHFESLGARVDEVSIPIHLDAQAIAMPILNYG
                     IVQTMLEGDGFGGGRNDLYVTSLMEFHRGWRGRIDEFSDNAKLWALIGGYVTERVGMH
                     HYGKAVNLSRKVRAAYDAVLEDCDLILAPTLPIKATPLPAADTGRAEKVARAMEMFAN
                     TVAADITHHPSMSVPCGLREGLPVGMMLTAKHFAETVIYRAAHAFERSKDWRTM"
     gene            2365..2550
                     /locus_tag="AC16QF_05045"
     CDS             2365..2550
                     /locus_tag="AC16QF_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447953.1"
                     /translation="MRFWAVAGTRLWWSVGEKVYYINLDLCWYYLSQHFQYGAGAIDD
                     VEWAQSLGCLEPDCLWG"
     gene            2594..3130
                     /locus_tag="AC16QF_05050"
     CDS             2594..3130
                     /locus_tag="AC16QF_05050"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIR6447954.1"
                     /translation="MSQKPLTDYIRTIADFPIPGIQFRDVTTLFGDAEGFRRAVDELA
                     EPYEGDGIDAVVAMDARGFILGGAVAYKLGLPFVPIRKKGKLPGKTIVEAYSLEYGDA
                     VVEIHDDALHAGARVLLLDDLLATGGTAVAGARLIERLGGHIVAASFVVNLPDLGGQK
                     RLEEMGLEVHVLCEFEGD"
     gene            3185..3346
                     /locus_tag="AC16QF_05055"
     CDS             3185..3346
                     /locus_tag="AC16QF_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447955.1"
                     /translation="MLNPQLGGVQSSEDDAAVLKADRMGQVIADRSAAAVDFDDDDRV
                     RMKLTLKAQ"
     gene            complement(3333..4079)
                     /locus_tag="AC16QF_05060"
     CDS             complement(3333..4079)
                     /locus_tag="AC16QF_05060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6447956.1"
                     /translation="MATCILFLAAVLLIGAAPGVQADQSFYTSKPASRDGIGKLYMGR
                     EIAHVMGHLGAGWLERPEREREERTDLLVARLPVEPDFVVADIGAGTGYFSFPIAERL
                     TDGEVLAVDIQPQMLAMIERQKAKRNIDNVSTVLGNEMDPGLPESGVDLIFIVDAYHE
                     FSHPREMGEAMFAALVPGGYLVLIEYRAEDASVPIKRLHKMSQAQTKKEMRAIGLEWL
                     RTEDYLPQQHVLVFQKPAVGSLAEPPVTAP"
     gene            complement(4266..4661)
                     /locus_tag="AC16QF_05065"
     CDS             complement(4266..4661)
                     /locus_tag="AC16QF_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447957.1"
                     /translation="MSDVASLWLLFGLPPAAPIVAALIAFAARDTGRWRWPLALSYVA
                     LVFCGAIATTNMAYHVREGYKNLPDVNWAVWAGLVWAGALTLPSAIALALRYKSMATA
                     LLTGLGVGVLSAITLISTSFGLMMMLSFE"
     gene            4831..5340
                     /locus_tag="AC16QF_05070"
     CDS             4831..5340
                     /locus_tag="AC16QF_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HIR6447958.1"
                     /translation="MQTPMENAMARMLATMAVGLGLWFATAGFTAAEEGAAMHLDVYK
                     SPTCGCCVKWMDHVAESGYTSTGHHPQDLGELKRELGVPARYGSCHTGVSQDGYVFEG
                     HVPARFITQFLADPPAGALGLTVPAMPVGSPGMEYKDQFMPYEVLLLNKDGSVEVFAS
                     VESYEQQFE"
     gene            5462..6172
                     /locus_tag="AC16QF_05075"
     CDS             5462..6172
                     /locus_tag="AC16QF_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447959.1"
                     /translation="MSEANASSPSTRVGTITCRCGEVTIALPNPVPKFRCGCCCTDCL
                     QRAYIGANGEPTAAIKNLEEAVDLLYVDSQIMRPGRETLARLSVFRLNKEDALNINLR
                     AECCGSVLCTENEKFHVPHSMATFNNLRPSVYCDFAAVPEPNCYVWTGDWPEERAEAL
                     ARREKSTKGEALPQIVDVMAALTEKPIIEIASALQLPAPTKAAGAISFAELCAGMEVK
                     IEHAYFQESRAHKVARKA"
     gene            6280..6696
                     /locus_tag="AC16QF_05080"
     CDS             6280..6696
                     /locus_tag="AC16QF_05080"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6447960.1"
                     /translation="MSRNLDQARLFFDACETGKGWEGCKEYCTPRATFSCQSGALADI
                     DSLEGYCEWMKNLLIPIPDGRYELKFFAEDEERACVAAFAVFHGTNTGPGGPGEPTGR
                     KAAADYVYAMDFDGDRIRHMTKIWNDGITLQELGWA"
     gene            6693..>7135
                     /locus_tag="AC16QF_05085"
     CDS             6693..>7135
                     /locus_tag="AC16QF_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR6447961.1"
                     /translation="MTASRQTPAKLDPGSEVRAKRSHEMAPGGRYSAMESRLQDGEIV
                     LLDGGTGTELEKRGAVMDPAAWCGPATLDNAEVLKDVHLDYISSGADIITTNTYASSR
                     LMLEPAGCGDRFEEINRAAVTAAHEARDLSGRADVLVAGSLSHMCP"
BASE COUNT         1384 a         2089 c         2278 g         1384 t
ORIGIN      
        1 atcggcaaag ccatcgatca gttctctggc atagccgcgc gcgtgatctt cgagcttagc
       61 cacttccttg gcattgaaaa aaggattcag cgcctgtctc caggcggtgt gatacggagg
      121 gtcgcagtct tccggcaaga tgggaatatt gccttccggc ggatcgagca tccagccatc
      181 cgcgctgctg aaagtgcgcc agtcctggcc tatctggcgt acatcgcgat agcggttgaa
      241 cacgcgatac ccgggaccca cctggctgcg tgcgaccgga cacttactaa ccagatgttc
      301 cagcacgtcc tcaaattgct ctgcgaacgc gctgtcttct atattgaaat cgccgtgcaa
      361 gtggtcgaag tcagacccca tctcgctccc cgctcatgac agtccttcag cccttcttcc
      421 tagtgcactt ctgctgcggg cgcaagccct tcagatggca agactcatgc tgcaagggag
      481 tggctcgacc agcttcggcg cgacgcgtaa tctgggtcca gcgcggggac cattggcgat
      541 tgctccgcca ggcttggcaa ccgtgtgctc ggaagcgaat caaaggaggt gctcatgccg
      601 attaaaagac ccagtcatgg cgaactgcga tcagttgctc aacgtctgaa cctcaccttg
      661 tccgaaaacg atgttgagac gtttcgggac ctgatggggg cgtcgatcga cgcgttcaat
      721 caggttgagg cgatggatga cgagctgcca ccggtgcggt acccgcggga ctccgggacg
      781 cggccgagtg cggctgacaa cccactcaac gcctggtact accgtaccag catccaaggt
      841 gcggatggtg gggcgctcgc gggacgaacc gtggcgctca aggacaacat catgctcgcc
      901 ggcgtcccga tgatgtgcgg ctcggccacc ctggaaggct acgtgcccga aatcgacgcg
      961 acggtggtgg aacgcattct ggacgcgggc ggcgagatcg ccggcaaagc gcactgcgag
     1021 ttcttctgtt tgtccggcgg cagccacacc aactcaaccg gtccggtgca caatccgcac
     1081 aaacgtggtt actcggcggg tggctcgtcc tccggcagcg cagccctggt ggcggcgggc
     1141 gatgtggaca tggcgatagg cggcgatcag ggcggttcga ttcgcattcc cgcgtctttc
     1201 tgtggtgtgt acggcatgaa gcctacgcac ggtctggtgc cctatacggg cgtgatgccc
     1261 ttggaggtct ttgtcgacca catcggtccg atgaccgcga acgtagagga caacgcgacg
     1321 ctcctcgagg cgatcgcggg tcccgacgcg tatgacccgc gtcagagagg cgttgaaacg
     1381 caccgttaca ccgaagccct ggggcaggac atcgcggggc tgaagatcgc cgtgctgaag
     1441 gaagggtttg ggcaccccaa ctccgaatcg gacgtggatg actgtgttcg cgcggccgcg
     1501 gcgcacttcg agtcccttgg cgcgcgggtg gacgaggtct cgattcccat ccatctcgac
     1561 gcgcaggcga tagcgatgcc cattctcaac tacggcatcg tgcagaccat gctcgaaggc
     1621 gatgggtttg ggggcggtcg caacgacctc tacgtgacca gcctcatgga gttccatcgc
     1681 ggctggcgag gacggataga cgagttctcc gacaacgcca aactttgggc gctgattggc
     1741 ggctacgtta ccgagcgtgt cggcatgcat cactacggca aggcggtcaa cctcagtcgc
     1801 aaggtgcggg cggcctacga cgcggttctg gaagactgcg acctgatact ggcgccgacg
     1861 ctgccaatca aggccacacc ccttccggct gcggataccg gtcgggcaga gaaggtggcg
     1921 cgcgcgatgg agatgtttgc caacacggtt gccgcagaca tcacgcacca cccatcgatg
     1981 agcgtcccgt gcggattgcg cgaaggcctt ccggtgggaa tgatgctcac ggcaaagcac
     2041 ttcgcagaaa ccgtaattta ccgggcggcc catgcctttg agcgttcgaa ggactggcgg
     2101 acgatgtagg ggaccccaag cgctccatga acgtcgagat aattgataag gcagcgtgtg
     2161 gccccccatg ggaccttgat tgttctgaag cggtcatttc catggtgcat ggcagatgtt
     2221 cgctcgctga ctggtaacct gtcagtcgtc cgatcactgt ggcgcgttcc ggacggattg
     2281 gcagacccga tgcgcggcag aacctgcagc aatctgcaat gcacgcagcg tggacggcat
     2341 ttttttcgat actagaatgc gggtttgaga ttttgggcag tagcgggcac ccgcttgtgg
     2401 tggtctgttg gggagaaggt ctactacatc aatctcgatc tctgctggta ctacctctct
     2461 cagcacttcc agtacggagc cggcgcgatt gatgatgtgg aatgggctca atcgctggga
     2521 tgtctcgagc ccgattgtct ttggggctga aaactctaag atcacgctca ggccgcaatc
     2581 acgagcaacg ggcatgagcc agaagccgct taccgactat atccgaacga tcgccgactt
     2641 ccccatcccc ggaattcagt tccgcgacgt cacgacgctt tttggcgacg ccgaagggtt
     2701 caggcgggcg gtggacgagc ttgccgaacc atatgagggc gatggcatcg atgcagtggt
     2761 ggcaatggac gcacgcggct tcattcttgg cggtgcggtg gcttacaagc tcggcctgcc
     2821 attcgttccc attcgcaaga aaggcaagct gcccggaaag accatcgtcg aggcctactc
     2881 cctggaatat ggtgacgcag tggtggagat tcatgacgac gccctgcatg ccggcgcgcg
     2941 cgtgctcctg ctggatgacc tgctggccac tggcggcacc gccgtcgccg gggccaggct
     3001 gatcgaacgg ctgggcggcc atatcgtcgc cgcctcgttc gtggtgaacc ttccggatct
     3061 gggcggccag aaacgtctgg aggaaatggg attggaggtg cacgtccttt gcgagttcga
     3121 gggagattga cgttaaagcc gcctgcgccc catacgtgag ccgtccactc tcagagcccg
     3181 ctgcatgctg aatccccaac ttggtggtgt tcagtcgtcc gaggatgatg cggcggtgtt
     3241 gaaggctgac aggatgggcc aggtgattgc ggatcgcagc gccgcggcgg tcgatttcga
     3301 tgacgacgac cgggtccgca tgaagctcac gctcaaggcg cagtgaccgg aggctcggcc
     3361 aacgacccga ctgcgggctt ctgaaacacc agcacgtgct gctgtggcag gtagtcctcc
     3421 gtgcgcaacc actcgaggcc gattgcccgc atctctttct tcgtctgtgc ctgcgacatc
     3481 ttgtgcagcc gcttgatcgg gacggaggcg tcttcggccc ggtactcgat cagcaccaga
     3541 tacccacccg gtaccagagc ggcgaacatg gcctcgccca tctcccgcgg gtgggaaaat
     3601 tcgtgataag cgtcgacgat gaagatgagg tcgacgcccg attcgggcag ccccgggtcc
     3661 atctcgttgc cgaggacggt tgagacgttg tcgatgttgc gcttcgcctt ctgccgctcg
     3721 atcattgcga gcatctgagg ctgaatgtcc acagcaagca cctcaccgtc tgtcaaccgc
     3781 tccgcgatgg ggaacgagaa gtagccggtg ccggcgccga tgtcggccac cacgaaatcg
     3841 ggttccaccg ggagtcttgc caccagcaga tccgttcgct cctcccgctc acgctcgggt
     3901 cgttccagcc aacccgcgcc caggtgcccc atcacgtggg caatctcgcg gcccatgtag
     3961 agcttgccga ttccgtcgcg gctggctggt tttgaggtgt agaagctctg atcggcctgc
     4021 acgccggggg cagcgccgat gagcagcact gccgcgagaa acaggatgca ggtagccaag
     4081 agggtttgcg tagttcgcat ctggtagggg cctggcagaa gtttggctgg ctagcttaaa
     4141 aaggcgaaag tgatcgttac gtgaatttag ccggtggctg cccgggtgcg ggtgcgtctg
     4201 atgccgatcc tgacgtccgc ctcttgcacg aaagcagcgc tgggcgttgg ggcggtgatc
     4261 gaaggctact cgaaagaaag catcatcatt agaccgaaac tggttgagat cagagtgata
     4321 gccgacagaa cgcctacacc caaaccggta agcagggccg tcgccatcga cttgtagcgt
     4381 agcgcaagcg cgatggcgct cggcagcgta agtgctcctg cccagacaag acctgcccac
     4441 acggcccagt tcacgtccgg cagattcttg tagccctctc tgacgtgata cgccatgttc
     4501 gttgttgcga tggcgccaca gaacaccaat gcaacatagc tcagcgccag cggccaacgc
     4561 cagcgccccg tgtctcgagc agcaaaggca atcagagcag cgacgatggg agctgcggga
     4621 ggcaggccaa agagcagcca aagactagcg acatctgaca tctgagcact cgtgttgcaa
     4681 gagctgatac tagcgggatc gcgcaggccg ggatgcgcgg cgttcgtcaa tctgagaacc
     4741 tgttgggatc ctgcggaggc gagtctcgaa ccccatctgc atgcttcctg ttgggcttgg
     4801 acgggcttcc ggcgttatga tcggctcctc atgcagacac cgatggagaa cgccatggct
     4861 cggatgctcg ctacgatggc cgttgggttg gggctgtggt tcgcaacggc cggattcacg
     4921 gcggcggaag agggcgccgc catgcatctg gacgtctaca agtcgcccac ctgcggctgt
     4981 tgcgtgaagt ggatggacca cgtggccgag agtggttaca ccagcaccgg tcatcacccg
     5041 caagacttag gtgagctgaa acgcgagctc ggcgtgccgg cccgctacgg gtcttgccac
     5101 acgggcgtga gccaggatgg ctacgtcttc gaaggccacg tgccggcacg gtttatcacg
     5161 caattcctgg ctgacccgcc tgcgggcgct ctgggcctca ccgtgccggc catgccggtt
     5221 ggcagcccgg gcatggagta caaggatcag ttcatgccgt acgaggtgct gctgctcaac
     5281 aaagacggct cggtggaagt gtttgcctcg gtggaatcct acgagcagca gttcgagtga
     5341 gttcgagcaa cctccggctt gagggatgct cgtcttcgat ctggcgcgtc cggtcctcgt
     5401 gaggtccacg atcgagacgg ttatagttcc gtccaggctt tccgcataca ggcgctgaga
     5461 gatgtcggaa gcgaacgcaa gcagcccgtc gaccagggtg gggacgatca cctgtcgctg
     5521 cggcgaggtc acgatcgcgt tgcccaaccc cgttcccaaa ttccgttgtg gctgctgctg
     5581 cacggactgc cttcagcgtg cctacatcgg cgccaacgga gaaccgaccg ctgcaatcaa
     5641 gaacctggaa gaggccgtgg atctgctgta cgtcgactcg cagatcatgc ggccgggccg
     5701 ggaaacgctc gccaggcttt ccgtgttcag gttgaacaag gaagatgcgc tcaacatcaa
     5761 cttgcgcgcg gaatgctgcg ggtcagtcct gtgcaccgag aacgagaaat ttcacgttcc
     5821 tcactcgatg gcgaccttca acaatctgcg tccttcggtg tactgcgact ttgccgccgt
     5881 gcccgaaccc aattgctacg tgtggaccgg agactggccg gaggagaggg cagaagctct
     5941 ggctcgaaga gagaagtcga cgaagggcga ggcgttaccc cagatcgtcg acgtcatggc
     6001 ggcgctcacg gaaaagccga tcatcgagat cgcttcggcc ctgcagctgc ccgccccgac
     6061 caaggcggca ggcgctatct cctttgctga gctgtgtgcc ggtatggaag tgaagatcga
     6121 gcatgcttac ttccaggaat cccgtgctca taaggttgcc cggaaggcgt gaccccatcg
     6181 ccgccccctc gggcggcggc gaccggagtc ccgtgcttta gaggtagggc gccgtcggtt
     6241 ttcgtggcaa gattgggcgg actgcaaggg gagcctgtca tgagtcgaaa tctcgaccaa
     6301 gcaagactgt tctttgatgc ctgcgaaacc ggcaagggtt gggagggttg caaagagtac
     6361 tgcactcctc gagccacgtt ctcgtgccag tccggggcgt tggccgacat cgattcgctg
     6421 gaaggatact gcgaatggat gaagaacctg ctgatcccga ttcccgacgg ccgttacgag
     6481 ctcaagttct tcgcggaaga tgaggaacgg gcctgcgttg cggccttcgc cgtgttccac
     6541 ggaaccaaca ccggccctgg cgggccgggt gaaccgacgg gccgcaaggc agctgcggat
     6601 tacgtctacg cgatggattt cgatggggat cgtattcggc acatgacgaa gatctggaat
     6661 gacggcataa ctctccaaga actggggtgg gcttgacggc ctcccgccaa acgcctgcga
     6721 agctcgatcc cgggtcggaa gtgcgtgcca agcgcagtca tgagatggcg cctggtgggc
     6781 gctactcggc catggagagt cgccttcaag atggagaaat cgttctcctg gatggcggca
     6841 ccgggaccga gctggagaag cgtggagccg tcatggatcc cgcggcgtgg tgtggaccgg
     6901 caactttgga caatgcagaa gtcctcaagg atgttcatct cgattacatc tcgtctggcg
     6961 ccgacatcat caccacgaac acgtacgcgt cctcacgatt gatgctggag cccgccgggt
     7021 gcggcgatcg tttcgaggag attaatcgtg ccgcggtgac cgccgctcat gaggcacggg
     7081 atcttagcgg ccgcgcagac gtgctcgtgg ccggttcgtt gtcgcacatg tgccc
//
LOCUS       DBNHLM010000110         5516 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1271768, whole genome shotgun sequence.
ACCESSION   DBNHLM010000110 DBNHLM010000000
VERSION     DBNHLM010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1271768"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="AC16QF_05090"
     CDS             <1..661
                     /locus_tag="AC16QF_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6447962.1"
                     /translation="DQRMSMFFGSRRNMKSVTAAEAAAIAAFCILDAGDRVGGVVFGD
                     DEHVEFVPRRSQRTVYGLLEAVTEMNQGLHADKTVDARPGSLNDILKSVARIAHHDHL
                     VLIFSDFDGIDEYTHRHLNGIAQHNDIIAMLVYDPMAEGLEPGQRAVIGDSRMQAEVD
                     LGSEATRTAVSGFSRQRLERIYQWQTEINLSVLPLSAGEPTLPQIRRLTGGMAPRRKV
                     R"
     gene            658..1140
                     /locus_tag="AC16QF_05095"
     CDS             658..1140
                     /locus_tag="AC16QF_05095"
                     /inference="COORDINATES: protein motif:HMM:NF025674.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HIR6447963.1"
                     /translation="MSAYDGLNLPQLLELMHELHLPEPISMVPQTPGWWVLAGWLAAI
                     ALMTAAHLAGRRRRNRYRREAQSSLDGIAARAADDPSGSAQEIAALLKRTALAAYPRA
                     TVASLYGADWAAFLCTTAGNDPEIAAAADGLAGSAYRADADGRALVSPAGRWIRLHRA
                     "
     gene            1133..2119
                     /locus_tag="AC16QF_05100"
     CDS             1133..2119
                     /locus_tag="AC16QF_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6447964.1"
                     /translation="MLDLASPWALLLTPLPLVIWWLLPPQREAVSALRVPFFDRIVAS
                     AGSQARAGAVIMRRRPLQMLVAATVWLLVVFGLARPEWVGEPIVRTEAARDIMLAIDL
                     SGSMDYRDFPDGDGRNIGRFEAVQKVVDRFIANRESDRVGMIVFGTKAYLQIPFTRDL
                     ESARALVKLMEVGMAGPKTALGDAIGLAIRTFESSEVDDRLLILLTDGNDTGSAMTPV
                     NAAEIAKLNDVEVYTIGIGDTEASGEDRVDFEALTEIAERTGGAFFNAEDASALTAIY
                     QRIDEAAVADVRTQSWRPRASLVHWPAGAAALLIILAYAALLIGARRRNVPA"
     gene            2116..3102
                     /locus_tag="AC16QF_05105"
     CDS             2116..3102
                     /locus_tag="AC16QF_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024909.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR6447965.1"
                     /translation="MMELLHFIRPLWLAALPAIVCVWWLVRRRDALQASAGRFLAPHL
                     SEALTINRNARRGLRAVDGVAVTAILLALATAGPTWSRQASPWYAETAPVVVAVEVSD
                     SMRANDLQPTRLDRARFKILDLIAARTGSRTALIAYAGSAHTVMPPTTDAEVLRPFLE
                     SLDPAIMPTGGANAAAVLPLARSLLGEAAATGTLLFVNDGFDPVDVPALAAFARDPGN
                     PPMAALVLGTEAGGVALMPDGSPVQSEGGDRLDTRVDASLLRRAESEGGVTIVRTGPG
                     DADVRRLLRVVESNLRQADDPDATWRDQGWWLLWPAALLSLLWFRRGWTMQW"
     gene            3096..3716
                     /locus_tag="AC16QF_05110"
     CDS             3096..3716
                     /locus_tag="AC16QF_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6447966.1"
                     /translation="MVARLVIITLAAAGILGVLTWQRGDFTGLWLTPDQQALKAYERR
                     EFRDAFDAFEDPAWKGVAAYRSGLYGESADAFGRIDSARGFYNRGNAFMKGREYGKAI
                     TAYEQAVADDPDWLEARENLALARHVLDYIERVREQSDTGDDTELGADDYVFDNTRKK
                     GVEIEITQESTIELESAEKWMRSVDTQTRDFLRSRFLLEAVRRGDL"
     gene            3716..4978
                     /locus_tag="AC16QF_05115"
     CDS             3716..4978
                     /locus_tag="AC16QF_05115"
                     /inference="COORDINATES: protein motif:HMM:NF052063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HIR6447967.1"
                     /translation="MLRLPLLVMLLGCTVLAAAAPAQPVVRVEVNPESVRVGESAELK
                     VIVLVPTWFARPPVYPTFELANAITRLPPDSSYPTSERVGRETWSGIVRNYRFYPLLG
                     ANYRLSGETITVTYANPGSTPLTVEVAVPEISIRGVVPAGAEGLNPYLAGRRLTLSRA
                     VESQAEPLRVGDAVVVTYAAELDGLPAIFLPPLLPELDVDGVSGYADVPKLGDGPPAR
                     REEKLTLVFDAGGTFTLPGTKLSWWNSESGRIETTSLEDLSFEVTGPLPSASVQTYDE
                     RALLGLAIAVTLVLVLGLIAWKAAPALAHTLQRAAEARRRSEPYAFRQLRRSIRAGNR
                     SAIHADLLAWLKKLNVEHDARAFARVYGNERLVAAIDSLSAHLYGRSQPDADPAELRR
                     GLVAARRRCQRSQGGADRAAGRLPALNP"
     gene            complement(5010..5087)
                     /locus_tag="AC16QF_05120"
                     /pseudo
     CDS             complement(5010..5087)
                     /locus_tag="AC16QF_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024851.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            5283..>5516
                     /locus_tag="AC16QF_05125"
     CDS             5283..>5516
                     /locus_tag="AC16QF_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HIR6447968.1"
                     /translation="MRMAIGFATLCIVLTLPSEANSAFGKKKSPEERRENITEMREDV
                     LNRLYDERASAKAQVQDSVGYAVFSNTGVNLFLL"
BASE COUNT          880 a         1907 c         1847 g          882 t
ORIGIN      
        1 ggatcagcgg atgtccatgt tcttcggctc ccggcgcaac atgaaatccg tcaccgccgc
       61 cgaagccgcc gccatcgccg ccttctgcat actggatgcc ggcgaccggg tcggcggcgt
      121 ggtgttcggc gacgacgagc acgtggagtt cgtgcccagg cgcagccagc gcacggtcta
      181 cgggctgctg gaagccgtca ccgagatgaa ccagggtctg cacgccgaca agaccgtgga
      241 cgcccgaccc ggcagcctga acgacatcct caagagcgtg gctcgcatcg cccaccacga
      301 tcatctggtt ctcatcttca gcgacttcga cggcatcgac gagtacaccc accggcactt
      361 gaacggtatc gcgcagcaca acgacatcat cgcgatgctc gtgtacgacc ccatggccga
      421 ggggctcgag ccgggccagc gggccgtcat cggggacagc cgcatgcagg cggaggtgga
      481 cctgggatcg gaggcgacgc gcaccgccgt cagcgggttc agccgccagc gcctcgagcg
      541 catctaccag tggcagaccg agatcaacct ttcggtgctc ccgttgagcg cgggtgagcc
      601 cacgctgccc cagatccgcc gactgacggg cggcatggct ccgaggcgca aagtacgatg
      661 agcgcttacg acggcttgaa cctgccccag ctcctggagc tcatgcacga gctgcacttg
      721 cccgagccga tctccatggt gccccagacc cccggctggt gggtgctcgc cgggtggctc
      781 gcggccattg cgctgatgac cgccgctcac cttgccgggc gccgccggcg caaccgctat
      841 cggcgcgagg ctcaatcctc gctggacggc atcgccgccc gggcggcgga cgatccgtcc
      901 ggaagcgccc aggagatcgc cgccctgctg aaacgaacgg cgctggccgc ttacccgcgg
      961 gcgacggtcg catcccttta cggagccgat tgggcggcct ttctctgcac aacggccggc
     1021 aacgatccgg agatcgccgc tgccgcagac gggctcgccg gcagcgccta ccgcgctgac
     1081 gcggacggcc gtgcgctcgt cagcccggcc ggtcgctgga tcaggctgca tcgtgcttga
     1141 cctggcctcc ccctgggcgc tcctgctcac accgctgccc ctggtgatct ggtggctgct
     1201 gccgccccag cgggaagcgg tgagcgcgct gcgcgtgccc ttcttcgatc ggatcgtagc
     1261 cagcgccggt tcccaggcgc gggccggcgc ggtgatcatg cgccgccggc ctctgcagat
     1321 gctcgtggcg gccaccgtgt ggctgctggt cgttttcggc ctcgcgcggc cggagtgggt
     1381 gggcgagccc atcgtgcgca ccgaggcggc gagagacatc atgctggcca tcgacctgtc
     1441 cggttccatg gactaccggg actttcccga cggcgacggg cgcaacatcg gccggttcga
     1501 ggccgtgcag aaggtggtgg accgcttcat cgccaaccgc gagagcgatc gggtcggcat
     1561 gatcgtgttc ggcaccaagg cctatctgca gataccgttc acgcgcgacc tggaatcggc
     1621 ccgcgccctc gtgaagctca tggaggtcgg catggccggt cccaagacgg ccctgggaga
     1681 cgccatcggg ctggccatcc gcaccttcga gtcgagcgag gtcgacgacc gtctgctcat
     1741 actgctgacc gacggcaacg acaccggcag cgccatgacg cccgtcaacg ccgcggagat
     1801 cgcgaagctg aacgacgtgg aggtctacac catcggcatc ggggacaccg aagcatcagg
     1861 cgaggaccgg gtggacttcg aggcgttgac agagatcgcc gagcgcacgg gcggcgcctt
     1921 cttcaacgcg gaggacgcct ctgcgctgac cgccatttac cagcgcatcg acgaagccgc
     1981 ggtggccgac gttcgcaccc agtcctggcg gccccgggca tccctcgtgc actggccggc
     2041 gggcgcggcg gcgctgctga tcatccttgc ctacgctgcg ctcctgatcg gtgcccggcg
     2101 aaggaacgtg cccgcatgat ggagctcctg cacttcatcc ggcccctgtg gctcgctgct
     2161 ctgccagcca tcgtgtgcgt gtggtggctg gttcggcggc gcgatgcgct gcaggcgagc
     2221 gccggccggt tcttggcgcc gcacttgagc gaggcgctga ccatcaaccg caatgccagg
     2281 cgcggcttac gcgccgtgga cggcgtcgcg gtcacggcca tcctgctcgc cctggccacg
     2341 gcggggccca cctggagccg acaggccagc ccctggtatg cggagaccgc gccggtggtg
     2401 gtggccgtgg aggtgtccga cagcatgcgc gccaacgacc tgcagccaac ccgcctggat
     2461 cgggcccggt tcaagattct cgacctgatc gccgcgcgta cgggatcgcg gacggcgctg
     2521 atcgcctatg ccggcagcgc gcacacggtc atgccgccca ccaccgatgc ggaagtgctc
     2581 aggccgtttc tggagagcct ggaccccgcc atcatgccca ccggcggggc caacgccgcc
     2641 gccgtgctgc ccctggcccg atcgctgctc ggcgaggcgg cggcaaccgg aaccctgctg
     2701 ttcgtcaacg acggcttcga ccccgtggac gttccggccc tggccgcctt cgcccgggat
     2761 cctggcaacc cccccatggc ggctctggtc ctcggcacgg aagccggcgg cgtagcgctc
     2821 atgcccgacg gatcgcccgt tcagagcgag ggcggcgatc gcctggacac ccgggtggac
     2881 gcatcgctgt tgcgtcgcgc cgagtccgag ggcggcgtca ccatcgtccg caccggtccg
     2941 ggggacgccg acgtccgccg gttgctccgc gtggtggagt cgaacctgcg ccaggcggac
     3001 gacccggacg ccacctggcg cgatcagggc tggtggctct tgtggccggc cgcgctgctg
     3061 tcgctgctgt ggttccgccg cggatggacc atgcagtggt agcccgactg gtaatcatta
     3121 ccctggcagc cgcgggcatc ctcggggtgc taacgtggca acgaggcgac ttcaccgggc
     3181 tgtggctcac tccggaccag caggccttga aagcctacga acggcgggaa tttcgcgacg
     3241 ccttcgatgc gttcgaggac ccggcgtgga agggcgttgc cgcctatcgc agcggcctct
     3301 acggcgagtc cgccgacgcc ttcggccgca tcgacagcgc ccggggcttc tacaatcgcg
     3361 gcaacgcctt catgaaaggc cgggagtacg gcaaggccat caccgcctac gagcaggccg
     3421 tggccgacga tccggactgg ctggaagcgc gggagaacct cgctctggcc cgccacgtgc
     3481 tcgactacat cgaacgggtc cgcgagcagt cggacaccgg cgacgacact gagctcggcg
     3541 ccgacgacta cgtcttcgac aacacccgca aaaaaggggt ggaaatcgag atcacccagg
     3601 agagcaccat cgagctcgag tccgccgaga agtggatgcg ctcggtggac acccagacgc
     3661 gggactttct gcgttcacgg tttctgctcg aagccgtccg gcggggtgat ctgtgatgct
     3721 gcgcctgccg ctcctggtga tgcttctggg ctgcacggtg ctggccgctg cggctccagc
     3781 gcagccggtg gtgcgcgttg aggtaaatcc agaaagcgtt cgggtcggcg aatcggcgga
     3841 gctcaaggtg atcgtgctgg ttcccacctg gttcgcccgg ccgcccgtct accccacgtt
     3901 cgagctcgcc aacgccatca cgcgcctgcc gccggacagc tcctacccca ccagcgagcg
     3961 ggtgggcaga gagacctggt ccggcatcgt ccgcaactac cgcttctatc cgctgcttgg
     4021 cgccaactat cgtttgtcgg gcgaaacgat caccgtgacg tacgccaatc cgggcagcac
     4081 gccgctcacg gtcgaggtgg ccgtcccgga gatatcgatc cgcggcgtcg ttcccgcggg
     4141 cgccgagggg ttgaacccct acctcgccgg caggcggctc accctttcgc gggccgtcga
     4201 gagccaggcc gagccgctcc gggtcggtga cgcggtagtg gtgacatacg cggccgagct
     4261 ggatggcctg cccgcgatct ttctgccgcc tctgctgccc gaactcgacg tcgacggggt
     4321 aagcggctat gcggacgtgc cgaagctggg cgatgggcct ccggcgcggc gcgaggaaaa
     4381 gctgaccctg gttttcgacg ccgggggcac gttcaccttg cccggcacga agctgtcctg
     4441 gtggaacagc gagtcggggc ggatcgagac taccagcctc gaggatctga gtttcgaggt
     4501 gaccggaccg ctgccgtcgg ccagcgtgca gacctacgac gaacgggcac tgcttggcct
     4561 ggcgatcgcg gtcacgctgg tcctcgtcct cgggctgatc gcgtggaagg ccgcaccggc
     4621 tctcgcccac accctgcaaa gggccgccga agcgcgtcgg cgctcggagc cctacgcctt
     4681 tcgccagctg cgccgaagca tccgcgccgg caaccgctcg gccattcacg ccgatctgct
     4741 cgcctggttg aagaagctga acgtggaaca cgacgcgcgc gcctttgccc gcgtttacgg
     4801 caacgaaagg ctcgtggccg cgatcgattc gctgagcgcc catctctatg ggcgctctca
     4861 gcccgatgcc gatccggcag agcttcggcg aggactggtg gccgcgcgcc gccgctgtca
     4921 gcgaagtcag ggcggggcgg accgcgccgc cggccggcta ccggcactga acccctgagc
     4981 cgatcgttcg agatccgaag cgagggccct caatactcga tgacgatggc gcccaccgga
     5041 cacgcgttgg cggcagactg cgcgtcgacc tcgagttcct cggggacttc tgcacgccga
     5101 cggcggagag gttcacttct tcaggatcca cgaccagacc tccccggctt tctcgaagac
     5161 gaccaaaaac gatgaaggct gcagaggccg ctgagaattg gcgtttccac ttacggctga
     5221 gcgcttatac ttggcacccc gtctgcgtga cgggattctt ttgtatcaga ggggaaccaa
     5281 ccatgcgcat ggccatcgga tttgcaacgc tctgcatcgt cctgaccctg ccgtcggagg
     5341 ccaactccgc cttcggcaag aagaagagcc ccgaggagcg tcgggaaaac atcaccgaga
     5401 tgcgcgaaga cgtcctgaac aggctctacg acgaaagagc atcggccaag gcacaggttc
     5461 aggattcggt gggttacgcc gtgttctcca acacgggtgt caatctgttc ctgctc
//
LOCUS       DBNHLM010000111         4702 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_15647, whole genome shotgun sequence.
ACCESSION   DBNHLM010000111 DBNHLM010000000
VERSION     DBNHLM010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_15647"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1301)
                     /locus_tag="AC16QF_05130"
     CDS             complement(72..1301)
                     /locus_tag="AC16QF_05130"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012076215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR6447969.1"
                     /translation="MREAWIIDAVRSPRGRGKPGVGSLSNIHPQRCMAQVLNGLRDKV
                     GFDASDIEDVVAGCGTGSGDHAHDIARMSVLDAGWPLTTTGVTLHRFCGSGQQAVTFA
                     AMGVMSGHQEAVIGGGVEFMSRYAPIHAMGFHANNEHLAALHPQVPQGISADLIATIE
                     GFSREDVDGYAVESQTRAARSIAEGRFEKALIPIFNDDGSPALDRDEHPRPGTTLESL
                     ADLKPSFAAMGSQQVEGFPKPFDEMARDRYPQVEEINHVHHAGNSSGVVDGASAVLVV
                     SPDFAKAHGLRPRARIRMTATAGTEPLIMLTAPAPAAQRCLQKAGMTRDDIDLWEINE
                     AFASVVLKFMKEMGVDHDVVNIDGGAIALGHPIGATGGMLIGTAVDQLEREDKETALV
                     AMCTGGGMGTATILERI"
     gene            complement(1396..2025)
                     /locus_tag="AC16QF_05135"
     CDS             complement(1396..2025)
                     /locus_tag="AC16QF_05135"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009394438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6447970.1"
                     /translation="MIDLYTWPTPNGRKISIALEEFGEPYRVHAVDIGAGDQHGSAFR
                     RISPNGKIPAIVEDSSGRSLMESGAILLYLAEKHDRFRGDDWWQTVEWLMLQMASVGP
                     MLGQVHHFARFNPGKAPYAEARYLDEARRLYGVLDRRLADREFLSGSYSIADMASWPW
                     ISRYEWQGIDLADYAHVRRWYVSIAGREAVQRGYHVPHFVNPVPMPDGA"
     gene            complement(2027..2794)
                     /locus_tag="AC16QF_05140"
     CDS             complement(2027..2794)
                     /locus_tag="AC16QF_05140"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6447971.1"
                     /translation="MIARRNSQRPNSAELLEFGEYVKVLEASGEREDVVAFFDLDRTI
                     IAGYSITALMIERLRSGMSPRHLVAQVSRFISYGFGRAGYQELLEGTVAELVGLPEQE
                     LVELGERAYQRRLQRLIYPEARRLIGAHKELGHEVVMVTSATRYQAQPVARELAVDQI
                     CCTELEIRQGRITGRVSPCFGASKRAAAVHLGSARACSLADAYFYTDSCDDLPLLEAV
                     GRPVAANPKASLSEVATSRGWPQLAFRRQGEAGSVAA"
     gene            complement(2906..3874)
                     /locus_tag="AC16QF_05145"
     CDS             complement(2906..3874)
                     /locus_tag="AC16QF_05145"
                     /inference="COORDINATES: protein motif:HMM:NF025598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HIR6447972.1"
                     /translation="MSARIGWLAALLAVQLLLTGALLLLEGRGADSSPAAFVEVAPDS
                     VDRVVVGGEETELEFTRAGEQWSLGSGAPADAEKIGEVLEKLTDIEPGWPVAATEASR
                     ERFRVTGDDHERRVALFAGEGKLAEIYLGTSPGFRRIHARRADSEEVYSIDFAVYELP
                     LSDSDWLDKDLLGADGEIASIIREEHWSLERGQEGWLLNLVPGMQDAEAGPGIGGADQ
                     DAAGRLVERVAELRVTGFAPLGPAYEPAAVFTVTDASGSYGLRIFKDPENDEYAVESD
                     RLSGRFGLAAYVAEQLVVEHADLRAAEPQDEAPAPGSEGFSDAGPG"
     gene            complement(3871..>4702)
                     /locus_tag="AC16QF_05150"
     CDS             complement(3871..>4702)
                     /locus_tag="AC16QF_05150"
                     /inference="COORDINATES: protein motif:HMM:NF021351.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HIR6447973.1"
                     /translation="FPAPVTRQVGGFSFQELVMLDYPYFVDVRGEGMNAESPIVAGLP
                     QVTLTWASPIEIDAEQQEGRTVTELLTSSPRSWLSADTNVMPRLDEQGLSGFAPEGAQ
                     APRLLGALIEGRFESFFAGQTSPLLEPADGDDDNDAGADGDDETDPAATVGVVSSVIA
                     RSADSARLFLFSSDGFLTDQNLRMLGSAEGTIYTNTVQMMVNVVDWSLEDQSLLSIRS
                     RGHFNRTLPPLVDEEQLFWEYLNYGLALLGIGVVFLVHRGRKRRSDRMYASWLAGGAS
                     "
BASE COUNT          810 a         1624 c         1486 g          782 t
ORIGIN      
        1 ctagcccggt gtggatcggc gggcacgcgc cgcgctgagc ggcgccttgg gataccccgc
       61 cggagccgac ctcagatccg ctcgaggatc gtagccgtac ccattcctcc gcctgtgcac
      121 atcgcaacca gcgcggtttc cttgtcctcg cgctcgagct gatccaccgc cgtgccgatg
      181 agcatgccgc ccgtcgcgcc gatgggatga cccaacgcga tggcgccacc gtctatgttg
      241 accacgtcgt gatcgacccc catctccttc atgaacttga gcacgacgga agcaaacgcc
      301 tcgttgattt cccacagatc gatgtcgtcc cgcgtcatgc ccgccttctg caggcaacgc
      361 tgggccgcag gagccggtgc ggtcaacatg atgagcggct cggttccggc ggtggccgtc
      421 atgcggatgc gggctcgggg tcgcagaccg tgagcctttg caaaatccgg gctcacgacg
      481 agcacggcgc tggcgccgtc aaccacgccc gaggagttcc cggcgtggtg cacgtggttg
      541 atctcctcca cctgagggta gcggtcgcgg gccatctcgt cgaacggctt gggaaatcct
      601 tccacctgct gagagcccat ggcggcaaac gagggcttca gatcagccag gctctccagc
      661 gtcgtcccgg gccggggatg ctcgtcccgg tccagcgcgg gcgaaccgtc gtcgttgaag
      721 atggggatca gggccttctc gaatcgaccc tcggcaatgg agcgagcggc acgggtctgg
      781 ctctccaccg cgtagccgtc cacgtcttcc cggctgaacc cctcgatggt ggcgatcaga
      841 tccgcggaga tcccctgagg aacctgggga tgcagggcag ccaggtgttc gttgttggca
      901 tgaaagccca tcgcgtggat gggcgcgtaa cgggacatga actccacccc gccgccaatc
      961 acggcttcct ggtgaccgga catcacgccc atggcggcaa aggtaacggc ctgctgtccg
     1021 ctgccacaga atcggtgcag ggttaccccg gtggtcgtca ggggccagcc ggcatccagc
     1081 accgacatgc gggcgatgtc gtgggcgtga tcgccgctgc cggtcccgca gccggcgacg
     1141 acgtcctcga tgtcggacgc atcgaaaccg accttgtccc tcagcccgtt gagcacctgc
     1201 gccatgcagc gctgagggtg aatgttgctc aggctgccga cccccggctt accccgcccc
     1261 cgcggtgacc gcaccgcgtc gatgatccag gcttcccgca tggcgcctct ccgttgaatg
     1321 tcagtggagc cgcgattcta caccgggcca accgaggctt caccggcgcc cgcggccacg
     1381 ccggcggcgt gacgctcagg cgccgtccgg catggggacg ggattgacga agtgcggcac
     1441 gtgataaccg cgctggacgg cttcgcggcc ggcgatcgag acgtaccagc ggcgcacgtg
     1501 ggcgtagtcc gcaaggtcga ttccctgcca ttcgtaacgc gatatccacg gccagctcgc
     1561 catgtccgcg atagagtagc tgcccgacag gaattcccga tccgccaggc gccgatcgag
     1621 cacgccgtat aggcgccgcg cttcgtcgag gtagcgcgct tcggcgtagg gggctttacc
     1681 gggattgaat ctggcgaagt gatgaacctg cccgagcatg gggccgacac ttgccatctg
     1741 cagcatcaac cactccaccg tctgccacca gtcgtcgccc cggaagcggt cgtgcttctc
     1801 agccaggtag agcaggatgg ccccggactc catcaggctg cgcccggacg aatcctcgac
     1861 gatggccggg atttttccgt tcgggctgat tcgtcggaag gccgagccgt gctgatcgcc
     1921 cgcgccgatg tccacagcgt ggacccggta gggctcgccg aactcttcca gagcaatcga
     1981 gatcttgcgg ccgttgggtg tcggccaggt gtaaaggtcg atcacctcag gcggccactg
     2041 agcccgcctc gccctgcctg cggaacgcga gctgcggcca gccgcgcgac gttgcgacct
     2101 cggaaagcga agccttgggg ttggcagcca cgggccgtcc gacggcttcg agaagcggca
     2161 ggtcgtcaca ggaatcggtg tagaaatagg cgtccgccag gctgcaggcc cgagcggacc
     2221 ccaagtgaac ggctgccgcc cgtttgctcg caccgaaaca cgggctgacc ctgccggtga
     2281 tcctgccctg gcggatttcc agctccgtgc agcagatctg gtcgacggcc agctcccggg
     2341 cgacgggctg cgcctgatac cgggtagccg aagtgaccat gacgacttcg tgacccagct
     2401 ccttgtgcgc gccgatcagg cggcgcgcct ccgggtagat cagcctctgc agacggcgct
     2461 ggtaggcccg ctcccccagc tccaccagct cctgctccgg cagccctacg agctcggcca
     2521 cggttccttc gagcagttcc tggtaaccgg cccggccaaa gccataggag atgaagcgac
     2581 tgacctgagc gacgagatgc cgtggcgaca tgccgcttcg aagccgctcg atcatgagcg
     2641 cggtgatgga atagccggcg atgatggttc gatccagatc gaaaaacgcg accacgtcct
     2701 cgcgctcgcc agacgcctcc agaactttga cgtactcgcc gaactccagc agctccgcgg
     2761 agttcggcct ttgactgttt cgccgggcaa tcatggcagg ccctggtcgc cgttggtcgg
     2821 tttagctctc acgcgccgcg ccagcgcggt gcgctccgca cgtcggcgac gcctgaacaa
     2881 tgagaccatc gaaacacgag tcgattcaac cggggcccgc atcggaaaac ccttcggacc
     2941 cgggcgccgg cgcttcgtcc tgcggttcgg cggcacgcag gtcggcatgc tctacaacca
     3001 gctgctcggc tacgtaagcg gccagaccga agcggccgga cagacgatcg gattccacgg
     3061 cgtactcgtc gttctccgga tccttgaaaa tccgcagccc gtaagacccg gatgcgtccg
     3121 tgacggtgaa gaccgcagca ggctcgtacg cggggcccaa cggcgcaaat ccggtcaccc
     3181 gaagctcggc gacccgctcg acgagccggc ccgcggcgtc ctgatcggcg ccgccgattc
     3241 cggggccggc ttcggcatcc tgcatccccg gcaccaggtt caggagccag ccttcctgac
     3301 cacgctccag ggaccagtgt tcctcccgga tgatcgaggc gatctcccca tctgccccca
     3361 gcaggtcctt gtcgagccaa tccgaatcgc tcaacggcag ctcgtagacg gcgaaatcga
     3421 tgctgtatac ctcctcggaa tccgcccgtc gggcatgaat gcggcggaat cccggcgacg
     3481 ttcccagata gatctcggcc agctttcctt cccccgcgaa gagggccacc cgtcgctcgt
     3541 gatcatcgcc ggtaacgcga aaccgctccc gcgacgcttc ggttgccgcc acgggccagc
     3601 cgggctcgat gtcggtgagt ttttccagaa cctcgccgat cttctccgcg tcggcagggg
     3661 cgccggagcc caacgaccat tgttcccccg ctcgggtgaa ctccagctcg gtttcctcac
     3721 cgccgacgac cacccggtcc acgctgtccg gggcgacctc cacaaacgcc gccggcgagc
     3781 tgtccgcgcc acgaccttcg aggagcagga gggcgccggt cagcaggagc tgaacggcga
     3841 gcagcgcggc aagccagccg atacgcgcgc tcatgaggca cctcccgcca gccagctcgc
     3901 gtacatccgg tcggaccggc gtttcctgcc ccggtggacg agaaagacca cgccgatgcc
     3961 cagcagcgca agcccgtagt tgaggtactc ccaaaacagc tgttcctcgt cgacgagcgg
     4021 cggcagcgtc cggttgaagt gtccgcgtga gcggatgctc aacaggctct gatcttctaa
     4081 ggaccagtcc acgacgttga ccatcatctg caccgtgttg gtatagatgg ttccctccgc
     4141 agagcccagc atgcgaaggt tctggtcggt caggaagccg tcggaggaga acaagaacag
     4201 tcgggcagag tccgccgagc gggctatgac gctgctcacc accccgaccg tggccgccgg
     4261 gtcggtctcg tcgtcaccgt ccgcccccgc gtcgttgtca tcgtccccgt cagcaggttc
     4321 gagaaggggc gatgtctggc cggcaaagaa agactcgaag cgcccttcta tcaacgcgcc
     4381 gagcagccgc ggcgcctggg caccttccgg ggcaaacccg gagagcccct gctcgtcgag
     4441 tctcggcatg acgttggtgt ctgccgacag ccagctgcgc ggtgagctgg tgagcagctc
     4501 ggtgaccgtt cgcccctcct gctgctcggc atcaatctcg atgggcgagg cccaggtgag
     4561 cgtaacctga ggaagcccgg ccacgatggg cgactcggca ttcattcctt caccgcgcac
     4621 gtccacgaag tacgggtagt cgagcatcac cagctcctga aagctgaacc cgccaacctg
     4681 tcgcgtcacc ggcgccggga ac
//
LOCUS       DBNHLM010000112         2866 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1512875, whole genome shotgun sequence.
ACCESSION   DBNHLM010000112 DBNHLM010000000
VERSION     DBNHLM010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1512875"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="AC16QF_05155"
     CDS             <1..304
                     /locus_tag="AC16QF_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447974.1"
                     /translation="SRRRFGAGTISGRRPMFVWSALDGGNGTPAQAVEYELAIAADRQ
                     ANPVSFRTHKMRYVPPDSLEACRTYSWKVRAHYLSFGEPTTSQWSPEYRFKTPCRK"
     gene            complement(358..1587)
                     /locus_tag="AC16QF_05160"
     CDS             complement(358..1587)
                     /locus_tag="AC16QF_05160"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6447975.1"
                     /translation="MSRDSSEVRRNWQLLVSACTGIICSSIVLPYYSIGALVVPITEE
                     FGWQRSQFQMAILFSSGLGALTAPVVGWLIDRYGARRIALPGILGLSVGFVLATFMDG
                     RLWMLYLSYGAMALLGAGTIPVTWTRAITTNFFRQRGLALGLTLSGTGLCAVIVPQYA
                     VWMVEAYGWRAAYLGLAAMPLLIAGPVVYFGFRPREGIGGEALETGEAQWGMTLGEAF
                     RTTKYWVLLISIFVVYMAVSGIGPNLIPALVDDGLTPAQAATALSTFGGAIIAGRIIV
                     GYLVDRFWAPGVAALSLSLPVAGCLMLVGAPGFETAVAAALLIGIAAGAELDLMSFLA
                     AKYFGLRHYAKIYAVLYATLAVCSGTAPMLFARVYDVTASYDVSFWISTVLFALGAVL
                     VLAMGAYPRMGDSRELP"
     gene            complement(1618..2496)
                     /locus_tag="AC16QF_05165"
     CDS             complement(1618..2496)
                     /locus_tag="AC16QF_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR6447976.1"
                     /translation="MILSTDGGGSRGLIAANVLYHMEKKAEIPIRETFDFSAGVSTGA
                     MVAAYLARNAGTMEEVAKKAYSSENLSKIMNKSIWDRILGRLQNQPKYDGQNERAYVS
                     SVTQDSRITDITDKFLLILAYDFINRELVTFRNNRGHDGWYNPSLTEVCDAASAAPTL
                     REIWVVSLGTGRPMRELSQDERDRIGEASRDWGIVGWVANGLLDHMMSASSCVSTHRC
                     EQLLGDHYVRVGGELPRKLMQLDNTSETRIADLQSYAFLWFEEHIGRVQQFLDDCDLA
                     RRRWGEEAKADGEYAV"
     gene            2600..>2866
                     /locus_tag="AC16QF_05170"
     CDS             2600..>2866
                     /locus_tag="AC16QF_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447977.1"
                     /translation="MTTFRAGALFLTMILVAVPLDAAARGVSSLGRIEPHDGVFQLVG
                     PSEIAVVAELLVDEGDLVKRGDVLARLDTYAIKAAEVKRVQVALD"
BASE COUNT          556 a          952 c          891 g          467 t
ORIGIN      
        1 ctcgcgccga cgattcggcg ctggcacgat ctccgggcgg cggcccatgt tcgtctggtc
       61 ggctctggac ggcgggaacg gcacacccgc ccaagctgtc gagtacgagc tcgccatcgc
      121 ggccgaccga caggccaatc cggtgagctt tcgaacccac aaaatgcgct acgtgccccc
      181 ggactccctg gaggcctgtc gaacctacag ctggaaggtt cgcgcccact acctgagctt
      241 tggcgagccg accacgagcc aatggagccc ggagtatcgg ttcaagacac cgtgcagaaa
      301 gtagatgtcg ccaaccctcg ccctgacacc acgctacgcg ccctgcatcg gagcggatca
      361 gggcagttcc cgtgaatccc ccatccgcgg atacgcgccc atggctagga cgagcacggc
      421 gcccagcgcg aacagaaccg tggagatcca gaagctcacg tcgtagctgg ccgtcacgtc
      481 gtacacccgg gcaaacagca tgggagccgt gcccgagcag acggccaggg tggcatatag
      541 caccgcgtaa atcttggcgt agtgacgcag accgaagtat ttcgccgcca gaaacgacat
      601 cagatcaagc tctgcacccg ccgcgatccc gatcagcagc gcggcggcca cggcggtctc
      661 gaagccgggg gcgcccacca gcatcaggca gccggccacg ggaaggctaa gggacagggc
      721 tgccactccc ggcgcccaga accggtccac gaggtaaccc acgatgatcc gcccggcaat
      781 gatcgcgccg ccgaacgtgc tgagcgccgt ggccgcctgg gcaggcgtca gcccgtcgtc
      841 caccagcgcc gggatgaggt tggggccgat gccggaaacc gccatgtaaa ccacgaagat
      901 ggagatcagc agcacccagt acttggtagt ccggaaagct tctcccaggg tcatacccca
      961 ctgagcctcg ccggtctcca gggcctctcc gccaatcccc tcccgggggc gaaagccgaa
     1021 gtacaccacg ggcccggcga tcagcagcgg catggccgcg agcccgaggt acgcggcgcg
     1081 ccagccgtaa gcttccacca tccagacggc atactgagga acgatcaccg cgcacagccc
     1141 cgtgccgctg agcgtaagcc ccagggcaag gccccgttgt cgaaagaagt tggtggtaat
     1201 ggcacgggtc caggtgaccg gaatcgtccc cgcgccgagc agggccatgg cgccataaga
     1261 cagatagagc atccacaggc gcccgtccat gaacgtcgcc agaacgaagc cgaccgacag
     1321 cccgaggatc cccggcaacg cgatgcgccg agccccgtag cgatcgatga gccagccaac
     1381 cacgggcgcg gtgagcgcgc ccaggccgga gctgaagagg atggccatct gaaactggga
     1441 gcgctgccag ccgaactcct ccgtaatggg caccaccagg gcgccgatgg agtagtacgg
     1501 tagaacgatg gagctgcaga tgatgcccgt gcacgcgctc accagcagct gccagttgcg
     1561 ccgaacctcg ctcgaatccc tgctcatgcc gatcggcgcc gcctgggcga acccggctca
     1621 gaccgcatat tcgccgtcgg ctttcgcctc ctcgccccag cggcgccggg ctaggtcgca
     1681 atcgtcgaga aactgctgaa cccgaccgat gtgctcctcg aaccacagaa aggcgtagga
     1741 ctggagatcg gcaatccggg tttccgacgt gttgtccagc tgcatcagct tgcgcggcag
     1801 ctccccgccg acgcgcacgt agtggtcgcc aagcagctgt tcgcaccgat gggtgctgac
     1861 gcacgagctg gcgctcatca tgtgatcgag tagaccgttg gccacccaac cgacgattcc
     1921 ccaatcccgc gacgcctcgc cgatgcggtc gcgttcgtcc tggctgagct cgcgcatcgg
     1981 cctgccggtg cccagcgaca ccacccaaat ctctctgagg gtgggcgccg cggacgccgc
     2041 atcgcaaacc tccgtcaggg acgggttgta ccagccgtcg tggccgcggt tgttcctgaa
     2101 ggtgaccagc tcccggttga tgaagtcgta ggcaaggatc agcagaaact tgtcggtgat
     2161 gtcggtgatc cgggaatcct gggtcaccga cgagacgtat gccctctcgt tctgcccgtc
     2221 gtacttcggc tggttctgaa gcctgccaag aatccggtcc cagatggact tgttcataat
     2281 cttggacagg ttctcggagg agtaggcttt cttggccacc tcttccatgg tgcccgcgtt
     2341 gcgtgcgagg taggcagcca ccatggcacc agtgctcacg ccagcggaga agtcgaacgt
     2401 ttcacgtatc gggatctcgg ccttcttttc catgtggtag agcacgttcg ccgcgatcag
     2461 accccgggaa ccgccaccgt cggtggagag aatcaagatt gctcctttgc tttgcatacc
     2521 gcccctcttg atcccattgt agccattagc cgctaacgta ccgtacaacg aacgccggga
     2581 aaatgccgca gggggaagca tgacgacgtt tcgagccggt gcgctctttc tgaccatgat
     2641 tttggtggct gtgccgttgg atgcagccgc ccgcggggtc tcgagcctgg gccggataga
     2701 gccccacgac ggcgtctttc agctcgtcgg gccttccgag attgccgtcg tggccgagct
     2761 tctggtcgac gagggcgacc tggtcaaacg gggcgatgtg ctggctcgcc tggataccta
     2821 tgcgatcaag gctgccgagg tgaaacgcgt tcaggtggcc ctcgat
//
LOCUS       DBNHLM010000113         7592 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_793265, whole genome shotgun sequence.
ACCESSION   DBNHLM010000113 DBNHLM010000000
VERSION     DBNHLM010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7592)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7592)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7592
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_793265"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="AC16QF_05175"
     CDS             <1..1123
                     /locus_tag="AC16QF_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HIR6447978.1"
                     /translation="DDVYHDQRVFADGVLPREYIEKSREFRGECVGIEPPHRIWSHVC
                     GSDLVRDSDGRVYVLEDNLRVPSGVSYMLENREVMKRVFPELFEDYVIHPVDDYPARL
                     ASCLRTLTSVKSPQIAVLTPGIYNSAYFEHAYLANAMAAQLVEGSDLVVGKDDYVYMK
                     TIDGLVRVDVIYRRIDDLFLDPDVFKPDSMLGVPGIVRAWRAGNVALANAPGCGVADD
                     KVIYTYVPDLVRYYLAEEPLLPNVPSYRCVDKEQRDYVLDNLDELVVKPANESGGYGM
                     LIGPKASRAERNRFARLIRANPRNYMAQPMLKLSTAPTLVDEHAEPRHLDLRPFILSG
                     ASSYVTMGGLTRVALRKGSTVVNSSQGGGSKDTWVVDLE"
     gene            1120..2124
                     /locus_tag="AC16QF_05180"
     CDS             1120..2124
                     /locus_tag="AC16QF_05180"
                     /inference="COORDINATES: protein motif:HMM:NF016086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HIR6447979.1"
                     /translation="MSGHTLSSTAKRTYWLGRYLERTESTARLIAENGNLMIDLPARL
                     PHSWSPLISITGSGATFATLYEEPTERNVTRFLIGDLRNHGSILSSLGWARENVRTLR
                     GIIPRQAVEYIIELHLYARDHLSGALSRSRRAEGLTVVPALIQQIEGFMSANMLHDAH
                     WNFWRLGNFVERADMTTRIIDVGTDNPLQTADAPGPFTDLQWRSVLRSLNATQSYLTV
                     RQEPIHQSAVVGFLIGNRSLPRSLTYSLESLRNGLRTLPRSDRALRAVNRLRRFLQSS
                     EIENLEGPELHQFLDGCQVQLAEIHQQIDRTYFEFHPRRIPQRADRSADTQPTRSDST
                     "
     gene            2121..2432
                     /locus_tag="AC16QF_05185"
     CDS             2121..2432
                     /locus_tag="AC16QF_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6447980.1"
                     /translation="MIRLYGMGRSNYFSIAKACLVERGMAFEEVATQPSAENAYLAKS
                     PMGKIPCIETERGFLSEAYAIVERIFGKDLLDSIPRARDLLALVGQQPSIQRVEAEKA
                     G"
     gene            complement(2442..3881)
                     /locus_tag="AC16QF_05190"
     CDS             complement(2442..3881)
                     /locus_tag="AC16QF_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR6447981.1"
                     /translation="MAWVADHWVAAVLFAVYTALLFYNANVGRRRSHDLTDYYVGGRG
                     MGGVVVGISFFATFASTNSYIGHAGKGYEYGLPWLTFAAMILIFTLISWTSVAPRLRR
                     FTLHWDAITLPDYLARRYGADNAALRVAAAVVILISSLLYLVAIFKGGGHLFQQFLGI
                     PYEGAVGLTLVIVMLYTSIGGFVSVVRTDVVQGILMVIGSVTIFYFVTRAAGGVGVIG
                     QLAERPGSEHVFTWSGGIPFAVLLGISLSGSLKLIVEPRQVSRFFALKDQRSVRVGTW
                     VALGGLTVIMACLFPVGFYAHFLLDGVQDTDMIVPILVSDPNVFPGWVADFLIVAMMA
                     AAMSSMDSVLLVSASVLYKDVVEVVHPAAHPLTWTRIGVVGLALLAAGVALRPPGGIV
                     EITIFSGSLYAVCFFPAILFGLHWRGGSAQAVLASMVSGVLVLVGWLLAGGREFAHEV
                     FPAMLVSCLSYAWVANVTRPALEHWPPEV"
     gene            3984..4190
                     /locus_tag="AC16QF_05195"
     CDS             3984..4190
                     /locus_tag="AC16QF_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447982.1"
                     /translation="MRYLVQLIIPVLIIVTVMFVVARNRRRRDEAGEQSDSEKGMFIF
                     ILVIGALVTVAIVVALQSFWGAGA"
     gene            4187..5032
                     /locus_tag="AC16QF_05200"
     CDS             4187..5032
                     /locus_tag="AC16QF_05200"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019579154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6447983.1"
                     /translation="MSFAVHPKPRGFGAAVTGIRLSDPISASLADELRAAWLAHQVLF
                     FPDQPMSHEDLERFTLCFGAFGNDPYVKAIPGHPHILEVRREPGENVAPFGASWHSDW
                     SFQAQPPAATILHAKIVPPVGGETWYADGYAAYEALPATLRDEISELTAIHSARRPYS
                     HQGFLAGRGPERSMTILPSDEALQTQDHPLVRTHPETGRNALWVNGVYTIGIRNMAEE
                     DAKNLLKRLFDHAIRDEFVYRHRWAPDMLTMWDNRSVQHCARGGYDGYRRVMHRTTVA
                     GDSPV"
     gene            5115..>7592
                     /locus_tag="AC16QF_05205"
     CDS             5115..>7592
                     /locus_tag="AC16QF_05205"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6447984.1"
                     /translation="MRAFSSFTAPLLMLLALSAFAQPTTDYLTPDLRARVERLKADVQ
                     QVPTNTTNVAERSRVLWNWANALSLDGGVLPVNLTQAVSFVFAYPDLMADRHDVIDGF
                     IQELTLLDEEPGALGTLRADSGPFQAASFVTLRQTFTVGSKAIQAGGGILVARHFQPN
                     YGAWQTGDPAADNYLSITTSAADASFTTETAPMIGMHGGFRRPADALVFRVASGTLRP
                     GDTVTLTYGDRSQGSRGFLMADFSSDMMPLPLYVAFTGSGRFFSLPIQPVAVIGGPAA
                     GVHAFAPSIVKAGEPFTLAVRAEDRFYNRASDGHPDWLVSLNGEPFREVPASEEPITL
                     LRNIEIAEPGVYRFTIASRDGQISGRGNPILVKQTAEERIFWGDTHGHSGFAEGIGTP
                     DFFMRWARDDARLDFVTHSEHDIWLDDGEWEQLRNNARSYTREGEFVAFLGYEWTVRN
                     THGGHHNVLFRTPAGRNRLAAPFYPSLSKLYAGLRGDAQPEDVLVIPHAHQAGDYRTS
                     DPELEHLVEVNSQHGNFEWFGQAYLRHGHQVGFTAASDNHLSQPGYGAPKNGTLAQRG
                     GLGAVIARARTTDALFDAMKQARAYATSGERIILEFSVNGTPMGQRGPFSETRTIEGR
                     VIGTAPIDDISLLRNGEEIWRRELLTDAADNTSSPGAFLLTFASDSDPRQPGDNPRGW
                     RHWRGTIEVRDATLIDASGMDFNNQLLQQIEADAGDPDLLRFSTITRGDTSSIRLDLE
                     DVSRATQVRVTLEPTQETGGAPATYGRHEQLPAAEVKLEIRDFENGETRAQLPAGPRE
                     DTITLRRIITQGSMDEAFTIDDVSGRQG"
BASE COUNT         1476 a         2597 c         2319 g         1200 t
ORIGIN      
        1 cgacgacgtc taccacgacc agcgcgtgtt cgccgacggc gtgcttcctc gagaatacat
       61 cgagaagtcc cgggaatttc gaggggaatg cgtcggcatc gagcccccgc accgcatatg
      121 gtcacacgtc tgcggctccg atctggtccg cgactcggac ggccgcgtct acgtgctcga
      181 ggacaacctc cgtgtgcctt ccggcgtttc ctacatgctg gaaaaccggg aagtcatgaa
      241 gcgcgtcttt ccggagctgt tcgaagacta cgtgatccac cccgtggacg actatccagc
      301 caggctcgcg agctgtctgc gcacgcttac ctcggtgaaa tccccgcaga tcgccgtgct
      361 cacgcccggc atctacaact cggcctactt cgagcacgcc tacctcgcca acgccatggc
      421 cgcgcagctc gtggagggct ccgacctcgt cgtcggcaag gacgactacg tgtacatgaa
      481 gaccatcgac ggcctggtcc gcgtcgacgt catctaccgt cgcatcgacg atctctttct
      541 ggatccggac gtcttcaaac cggattccat gctcggcgtc cccggcatcg tgcgcgcctg
      601 gcgcgcgggc aacgtggcgc ttgccaacgc gcccggctgc ggcgtggcag acgacaaggt
      661 gatctacacc tacgtccccg acctcgtccg ctactacctg gccgaggagc ctttgctgcc
      721 caacgtgcct tcctaccggt gcgtggacaa ggagcagcgt gactacgtgc tcgacaatct
      781 ggacgagctg gtggtgaagc ccgccaacga gtccggaggc tacggcatgc tgatcgggcc
      841 caaggcgtca cgggcggagc ggaaccggtt cgccaggctt attcgcgcga acccgcgcaa
      901 ctacatggcc cagcccatgc tcaagctctc caccgcgccc acgctggtcg acgagcacgc
      961 cgaaccccgg catctggact tgagaccctt cattctctcc ggcgccagca gctacgtgac
     1021 catgggcgga ttgacccggg tcgcgctgcg caagggctcg accgtggtca actcgtctca
     1081 gggcggcggc agtaaagaca cctgggtggt ggacctggaa tgagcggcca tacgctgtcg
     1141 agcacggcaa agcgcaccta ctggctggga cgctacctgg agcgcaccga gagcaccgca
     1201 aggctgattg cggagaatgg caacctgatg atcgatctgc ccgcacggct cccccacagc
     1261 tggtcgccgc tgatcagcat caccggaagc ggagcgacgt ttgccacgct ctacgaagag
     1321 cccacggagc gcaacgtcac ccgcttcctg atcggcgacc tgcgcaatca cggcagcatc
     1381 ctgtcatcgc tgggctgggc cagggaaaac gtgaggaccc tgcgcgggat catcccgcgg
     1441 caggccgtgg agtacatcat cgagctgcat ctgtacgcgc gcgaccactt gagcggcgcc
     1501 ctgtcacgct cccgtcgagc cgaagggcta accgtggtcc ccgcgctgat ccagcagatc
     1561 gaaggcttca tgtccgcgaa catgctgcac gatgcccact ggaatttctg gcggctcggc
     1621 aacttcgtgg agcgagccga catgaccacc cgcatcatcg acgtgggcac cgacaaccct
     1681 ttgcagaccg cggacgcgcc ggggccgttc accgacctgc aatggcgcag cgtgctgcgc
     1741 tctctgaacg ccacgcaaag ctacctgacc gtacgccagg agcccatcca tcagagcgcc
     1801 gtggtcggat ttctcatcgg caatcgctcg ctgccgcggt ccctgaccta ctcgctggag
     1861 agcctgcgca acggtctgcg cacgctgcct cgcagcgacc gcgccctgag ggccgtcaac
     1921 aggctgcgca ggttcctgca gagcagcgag atcgagaacc tcgagggccc tgaacttcac
     1981 cagtttctgg acggctgcca ggtacagctg gcagagatac accagcagat cgaccggacc
     2041 tacttcgagt tccatccacg gcgcataccc caacgggcgg atcgttccgc agacactcaa
     2101 ccaaccagga gcgacagcac atgatcaggc tctacggcat gggacgcagc aactatttca
     2161 gcatcgccaa ggcgtgtctg gtggagaggg gcatggcgtt cgaggaagtc gccactcagc
     2221 cgtctgcgga gaacgcctac ctcgccaaga gccccatggg caagatcccc tgcatcgaga
     2281 cggaacgggg cttcctttcc gaggcatacg ccatcgtcga aagaatcttc ggcaaggatc
     2341 tgctcgactc gataccgcgg gcccgtgacc tgctggcgct cgttggccag caacccagca
     2401 ttcagcgcgt agaggccgag aaagcgggct gagcgacgac gtcagacctc ggggggccag
     2461 tgctcgaggg ccgggcgggt cacgttggcg acccacgcat agctgagaca cgagaccagc
     2521 atggcgggga acacctcgtg agcgaactcc cttccgccgg cgagcagcca gcccaccagc
     2581 acgagcacgc cgctgaccat ggacgcaagc accgcctggg ctgaacctcc gcgccagtgc
     2641 agaccaaaca gtatggccgg gaagaagcag acggcgtaga gactgcccga aaatatcgtg
     2701 atctcgacga tgccgcccgg cggccgcagc gctaccccgg cagccagcag cgcaaggccg
     2761 accacaccga tgcgcgtcca ggtcagcgga tgggcagccg gatggacgac ctcgaccaca
     2821 tccttgtaga gaaccgaggc ggaaaccagc agaacgctgt ccatggagga catggcagcc
     2881 gccatcatgg cgacgatgag aaagtccgcg acccacccag gaaacacatt cgggtcgctg
     2941 accaggatcg gaacgatcat gtccgtgtcc tgcaccccgt cgagcagaaa gtgggcgtag
     3001 aaacccacgg gaaacagaca ggccatgatg acggtcaggc cgcccagcgc gacccacgtc
     3061 cccacccgca cgctgcgctg atctttcagc gcgaagaatc gcgaaacctg gcgcggctcg
     3121 acgatcagct tcagagaacc cgagagggaa atgcctaaca gaacggcgaa cgggataccg
     3181 ccgctccagg taaaaacgtg ctcactcccc ggccgctcgg ccagctggcc aatcaccccc
     3241 acacccccgg cagccctggt gacgaagtag aagatggtca ccgacccgat gaccatcaga
     3301 attccctgaa ccacgtcggt gcgcaccacc gagacgaagc cgccgatgga ggtgtagagc
     3361 atgacgatga ccagcgtcag accgacggct ccttcgtagg ggatgccgag gaactgctga
     3421 aacaggtgcc cgcccccttt gaagatggcc accagataga gaagactcga gatcagtatc
     3481 accacggccg ccgcgacccg caacgcggcg ttgtccgctc cgtagcgacg ggcaaggtag
     3541 tcgggcagcg tgatcgcatc ccagtgaagg gtgaagcgtc gcagccgggg cgccacggag
     3601 gtccacgaaa tcagggtgaa gatcagaatc atggccgcga acgtcagcca cggcagaccg
     3661 tactcgtagc ccttgcccgc atgaccgatg tagctgttgg tgctggcaaa ggtcgcgaag
     3721 aaggaaatgc ccaccacgac gccgcccata ccgcgaccgc cgacgtagta atccgtcagg
     3781 tcgtgacttc tcctgcgccc cacgttggcg ttgtagaaca gcagcgccgt gtagacggca
     3841 aaaagcacgg cagcaaccca atggtcggcg acccacgcca tgcgaacact acccttcccg
     3901 gcctgacccg gccaaagcgt accattgatc gctggcggac cgtctcgtta tgctcgaacg
     3961 ccacaagccg cgggaatccg tccatgcgtt acctggtaca gctcatcatc ccggttctga
     4021 tcatcgtgac ggtgatgttc gtggtcgccc gcaaccgccg ccgccgcgat gaggcagggg
     4081 agcaatcaga cagcgagaag gggatgttca tcttcatcct ggtcatcggt gccctggtga
     4141 cggtggccat cgtcgtcgcc ttgcaatcct tctggggggc cggggcatga gcttcgccgt
     4201 gcatcctaag ccgcgggggt ttggcgccgc cgttaccggt atccggcttt ccgacccgat
     4261 cagcgcctcc ctggccgatg agctcagagc ggcctggctg gcccatcagg tgctcttctt
     4321 ccccgaccag cccatgagcc acgaggacct ggaacgattt actctgtgct tcggcgcttt
     4381 cggaaacgac ccctacgtga aagccatccc cggtcatccg cacatcctgg aagtcaggcg
     4441 cgagcctggg gagaacgtcg cccctttcgg ggcgtcctgg cactccgact ggtccttcca
     4501 ggcccagccg ccggccgcaa ccattctgca cgccaagatc gtccccccgg tgggcggcga
     4561 gacctggtac gcggatggct acgcggctta cgaggccctg ccggcaaccc tgcgcgacga
     4621 gatctccgag ctcaccgcga tccacagcgc ccgccgcccc tacagccacc agggattcct
     4681 ggccgggaga ggcccggagc ggagcatgac catcctgccc agcgacgaag cactgcagac
     4741 ccaggaccat ccgctcgttc gcacgcaccc cgaaaccggc cgcaacgccc tgtgggtgaa
     4801 cggcgtctac accatcggca ttcggaacat ggcggaggaa gacgcaaaga atctgctgaa
     4861 gcggttgttc gatcacgcca ttagggacga gttcgtctac cgtcaccgct gggcgcccga
     4921 catgctgacc atgtgggaca accgctcggt tcagcattgc gccaggggcg gatacgacgg
     4981 gtatcgccgg gtcatgcacc gcaccaccgt ggccggcgac tcgccggtct gaggcccggc
     5041 cggcgattgc gctaccctcc cacgccgcca tgtgagaagc tcgccccggt ccagtgacag
     5101 tggagccctg acgcttgcgc gcctttagtt cgttcaccgc gcccctgctc atgctgctcg
     5161 ccctgtcggc tttcgcgcag cccaccacgg attacctgac gcccgatctg cgcgcgcgcg
     5221 tcgaacggct gaaggccgac gtccagcagg taccgaccaa caccaccaac gtcgcggagc
     5281 ggtcccgcgt gctttggaac tgggccaatg ccttgtctct cgacggtggt gtgctgccgg
     5341 tcaacctgac ccaggccgtc tctttcgtgt tcgcctatcc ggacctgatg gccgatcggc
     5401 acgacgtcat cgacggcttc atccaggagt tgaccctgct cgacgaggag cccggggcgc
     5461 ttggaaccct tcgagccgat tcgggaccct tccaggcagc gtccttcgtc acgctgaggc
     5521 agacgttcac cgtcggttcc aaggcgatac aggcgggcgg aggcattctg gtggccaggc
     5581 atttccagcc caactacggc gcgtggcaga cgggcgatcc agccgcggac aactacctgt
     5641 caataaccac cagcgctgcc gacgcgagtt tcaccaccga aaccgccccg atgatcggca
     5701 tgcacggcgg tttccgccgt ccggctgacg cgctcgtctt tcgcgttgcc tccgggacgc
     5761 tgcgcccggg cgataccgtg accctgacct acggtgaccg cagccagggg agccgcggat
     5821 tcctgatggc cgatttttcc tccgacatga tgccgttgcc cctgtacgtg gcgttcaccg
     5881 gatccggacg cttcttctcg ctacccattc aacccgtggc ggtgatcggc ggcccggcgg
     5941 ccggcgtgca cgccttcgcg ccgtctatcg tgaaggccgg agagcccttc accctcgcgg
     6001 tccgggccga ggaccgcttc tacaaccgcg cctcggacgg acatccggac tggctggttt
     6061 ccctcaatgg cgaaccgttc cgggaggtgc cggccagcga ggagccgatc accctgttgc
     6121 gcaacatcga gatagccgaa ccaggcgtat atcggttcac catcgcgagc cgggacggcc
     6181 agatcagcgg acgtggaaat ccaatcctgg tcaagcagac ggccgaggag cggatattct
     6241 ggggcgatac ccacggccac tcgggattcg cggagggtat cggcacgccc gacttcttca
     6301 tgcgctgggc gcgcgacgat gcgcgcctgg acttcgtcac ccattcggag cacgacatct
     6361 ggctggacga tggcgagtgg gagcagctgc gcaacaacgc ccgcagctac acccgggaag
     6421 gcgagttcgt cgccttcctc ggctacgagt ggacggtccg gaatacccac ggcggacacc
     6481 acaacgtgct gttccgcacg cccgccggcc gcaatcggct ggcagcgccc ttctacccgt
     6541 ccctgtcaaa gctttacgca ggcctgcgcg gtgatgccca gccagaggac gtgctcgtca
     6601 tcccccacgc gcaccaggcc ggcgactacc gcaccagcga cccggaactc gagcacctcg
     6661 tggaggtcaa ctcccagcac ggcaacttcg aatggttcgg tcaggcctat ctgcgccacg
     6721 ggcaccaggt gggcttcacc gccgcgtcag acaaccatct cagccagccg ggatacggtg
     6781 ccccaaagaa cgggacactt gcccagcgag gcggtctggg cgccgtcatt gcccgggcca
     6841 ggaccaccga tgcgctgttc gacgccatga agcaggctcg cgcctatgcg acttccggcg
     6901 agcgcatcat cctggagttc tcggtgaacg ggacgcccat ggggcagcgc ggacccttct
     6961 ccgaaacccg cacgatcgaa ggtcgggtga tcggcacggc gccaatcgac gacatctcgt
     7021 tgctgcgaaa cggcgaggag atctggcgcc gggaactgct gaccgacgct gccgacaata
     7081 cgagctctcc gggagctttc ctgctgacct tcgcctccga ctccgatccg cgacagccgg
     7141 gagacaatcc gcgagggtgg cgccactggc gtggaaccat cgaggtgagg gacgcgacgc
     7201 tcatcgatgc cagcggcatg gatttcaaca atcagcttct ccagcagatc gaagcggacg
     7261 caggggatcc ggacctgctt cgtttctcga ccatcacccg cggggacacg agcagcattc
     7321 ggctcgacct ggaggacgta tccagagcca cgcaggtccg cgttaccctg gaacccaccc
     7381 aggaaaccgg cggcgcgccg gccacgtacg gccgccatga gcagctgccg gccgcggagg
     7441 tgaagctgga gataagagat ttcgagaacg gcgagacccg ggcgcagctg cccgccggtc
     7501 cacgggagga caccatcacc cttcgccgga tcatcaccca gggaagcatg gacgaagctt
     7561 tcaccatcga cgacgtcagc gggcgccagg gt
//
LOCUS       DBNHLM010000114        36996 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1632255, whole genome shotgun sequence.
ACCESSION   DBNHLM010000114 DBNHLM010000000
VERSION     DBNHLM010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 36996)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 36996)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36996
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1632255"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="AC16QF_05210"
     CDS             <1..674
                     /locus_tag="AC16QF_05210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6447985.1"
                     /translation="HPVVLAEQIATLASIAPGRFIMQCGLGDDRQGKAMGVDMSRRVG
                     MFRASLEVMRALWRGESVDEERYWHLCGARIAPLPPEPIEVWIGALVQPAIRRAAQLG
                     EGWLASPNLTPAQTADAANVYRQACAEFGRSPAAVALRRDIFVGATSQAARATVTPYV
                     EAGYRGMSEDALLFGSPGEVADQLRSFADQGYTDVIVRNLSARQDESLATIERLAEVK
                     ALLTG"
     gene            712..1401
                     /locus_tag="AC16QF_05215"
     CDS             712..1401
                     /locus_tag="AC16QF_05215"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6447986.1"
                     /translation="MYRLHGATSSPFVRKTWIFMKEKGLEFEHCELDPLDKSQRFLAM
                     NPLGRIPVLEEPDGNFIADSSVICDYLERCHPGPALYPSNNRDRARALWLEEYADTAL
                     NQVCARIFWMHIIIPFRTGRPADQGEIDAFKEEDYPGVFDYLDRAAPETGDGMVGGRF
                     GIADMALAAPVRLLDLAGDPLDATRWPRFDAHYQRVVARPSAQSIVAKDLATTEVWRT
                     TGNAPASTQSG"
     gene            complement(1398..2222)
                     /locus_tag="AC16QF_05220"
     CDS             complement(1398..2222)
                     /locus_tag="AC16QF_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447987.1"
                     /translation="MLGLVLLNGIGVCSMAQADEKPVPRDAGLEFVYDDGFTESERRK
                     LEAWIAEVAGALEVLVGPLPMDVRFRFARHEGAGPVPWANTLRGRRQGVRLYVDPTQP
                     VEAFRNDWTAPHELSHLVLPFVGREHAWFSEGFASFMQFRVMRIMDVLDDEAMAARYS
                     ERLDTAAAAYSQPNRRFVEAVPQLRAEGLYPTIYWGGASYFLEMEERLKRKGASLVGV
                     LAAYLACCRRNEDDLEDLVARLDDLAGSTTFSDGLTGFRRVVGFPPHRHLQALAPR"
     gene            complement(2246..3892)
                     /gene="betA"
                     /locus_tag="AC16QF_05225"
     CDS             complement(2246..3892)
                     /gene="betA"
                     /locus_tag="AC16QF_05225"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF002550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HIR6447988.1"
                     /translation="MEVFDYIIAGAGSAGCVLANRLSEDGARSVLLLEAGGSDRHLFV
                     RMPSAFSLPIGRPRFDWCYQAEAEPALDGRSLACPRGRVLGGSSSINGMVYVRGHPRD
                     FERWESLGATGWGYADVLPYFKRAEHVLGAAEGDPYRGRAGPLTVSRGRRANPLYETF
                     LQAAEQAGHALSDDLNGYRQEGFGDLEMTVRDGVRCSAALAYLAPAVSRSNLTVRSGS
                     PVQRLNVESGRCEGLTYRHRGATHTAVAREEVVLAAGAIGSPQLLMLSGIGHGEALEP
                     HGIPVRHELPGVGANLMDHLEIYFQQACTQPVSLNRWLNPLGKGVIGARWLLTASGPG
                     ASNHFETGGFVRSAADVPWPDVQFHFLPAAVSYDGRSVADADGFQVHVGPMLSPSRGR
                     LTLRSADPDVAPVISFNYMSCEEDWQVFRRAIRLSRELFAQRAFDPYRGVEIAPGSGA
                     DSDGAIDAFVRRSTESAYHPCGTCRMGNDPMAVVDSECRVRGIQGLRVVDASIFPHIT
                     NGNLNAPTIMTAEKAADAILGRRLAAEALGYFDAPASPTR"
     gene            4022..5125
                     /locus_tag="AC16QF_05230"
     CDS             4022..5125
                     /locus_tag="AC16QF_05230"
                     /inference="COORDINATES: protein motif:HMM:NF013394.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylethanolamine N-methyltransferase
                     family domain-containing protein"
                     /protein_id="HIR6447989.1"
                     /translation="MVSGSLLAGWFAPLAVYLAILILHLALPARRVDGYALDPGTGQP
                     LTYRLNGLVVFALVTSVWLAAGWKGWISWDWLYVNRWSAMGGACLVGLAYSLITVLGA
                     PPTGRSLLADLYLGRPENPQHFNKRVDAKMFLYLAGAVLLGLNTLSATAFHAGALGSA
                     SNPGVYLHAALLTFFVLDYLVFERVHLYTYDLFAERVGLKLGWGCLAFYPFFYPVGLW
                     GTAHLGKPSVIADLGWIWLALSALVFLSGWVLARGANLQKYVFKRFPERAFLGRFPPR
                     VIHGGNLALLCGGFWGVARHVNYLGEILMATGLAMSLGHLDSPWPWLYPLYYLALLVP
                     RERDDDRRCAEKYGTLWDEYRRRVPYRIVPRIY"
     gene            5149..5643
                     /locus_tag="AC16QF_05235"
     CDS             5149..5643
                     /locus_tag="AC16QF_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013981.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HIR6447990.1"
                     /translation="MNLDLILALKVLALALSANGAPVIGKRLFGARWAIPLDGGLTFL
                     DGRPLLGSSKTVRGLVLSLATTTLVATALGYGWQLGVIYSALTMSGDALSSFVKRRRG
                     IPPSGRFRGLDQIPETALPMWVCSSALGLTGWDIAVLVALFFAGDFVLSKVLYRIGIR
                     DEPY"
     gene            complement(5637..6602)
                     /locus_tag="AC16QF_05240"
     CDS             complement(5637..6602)
                     /locus_tag="AC16QF_05240"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6447991.1"
                     /translation="MRDSEILQRLEKRLGTAHARERLGVETAHESRLFSHGIRFFHPE
                     NWYSVHSIIRNSLKVSGLYARASRNALDHRVVENHVVLDALPAGLDGLRVLHLSDLHV
                     DVSDELLHAIIERVRELTYDVCVLTGDYRFRTYGAIDDALRGMARLRSNLDGSVYAVF
                     GNHDSVTMLPALEDMGIRMLMNESHAHRHGDDHLYLAGIDDAHFFGVHDIEKALAGVP
                     DTVPRILLSHTPEVYRQAARAGVQLLLCGHTHGGQICLPGGYPVTLDSRVPRRLGRGC
                     WDYKGMRGYTSPGAGASMVAVRLNCPPELTVHVFHSAGPGAAQLQ"
     gene            complement(6647..7447)
                     /locus_tag="AC16QF_05245"
     CDS             complement(6647..7447)
                     /locus_tag="AC16QF_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6447992.1"
                     /translation="MSDSEIVLCEIDDGTATLILNRPEVLNAMNDELMGGICRAMERV
                     VEDESVRVVILTGAGRGFCAGADLARAAEGPADPDLQDAPDSGDLFNGAVSALMSCPV
                     PTIARVNGPAAGGGLGLALACDVAIAAESAIFVATFGPNLGIVPDLGSTWSIPMRVGR
                     ARALGMTLLGDRITANQAEAWGLIWRSVPDEELDAEVGRVAMVLKRSSPSACTRIREC
                     VDAAIHHTFGDQLGLEMAHQGVLIPRNMQEGARAFMEKRPPEFGGDRG"
     gene            complement(7569..8309)
                     /locus_tag="AC16QF_05250"
     CDS             complement(7569..8309)
                     /locus_tag="AC16QF_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447993.1"
                     /translation="MGRHSVIFLMAALLMLGSSGLRAEQGDETWWSITPYIWAMDTKL
                     DLSWRDSPVGGTEIDFKDALGNLDAAFQVAVEGGRGRWSAFADVTFVKISDATGEDAL
                     RLKTKNKQVFLDAGVAFWPGGAGSPLSIYGGARYTGFDNTYRLQFQGDDVARRDSSAD
                     YFDALLGVRYRFDLSDRWSILTLGDVSFGESEGSWQAQALAAYTVGKRRMNRIMFGYR
                     YRDTEFDDAELTTEYGFHGPLVGFNFRF"
     gene            8492..9838
                     /locus_tag="AC16QF_05255"
     CDS             8492..9838
                     /locus_tag="AC16QF_05255"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017773.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6447994.1"
                     /translation="MITERANPSLRLFAAVAMLAALTACAAKGPYVLELMPAPTVYQD
                     EDFSPFADDFSPESLPYRGMLYVTDRRPAGAGKPPDPADPHYVGKRGEGLRAGVASIQ
                     LGEGGFTWEEAIRVSMASDRGEGYRLSVTGVEELGFLEPSIEPFIEAPDFPLADEAFA
                     ALVNDKLAQSEQKDVYIYVHGYRVRFEYPVLVATELWHFLGYEGAFIAFSWPSTPSYL
                     AYFADAEVAVVSARQLRILLEFLAQSTDVRRIHILGYSQGTRTVMQALHMLALKYEED
                     DRESLLERLRIGNVALVGSDIDRETMGMYVADGLMRVTERMTVYASAKDKALRISRRV
                     LRRDRIGQLIPSDEITDELRGFLITTPEFEIINVTDAEDATTENGHRYFRSSPWVSSD
                     LLTSFRYGMTPGQRGLEILPETPIWQFPADYPTRLRSALNDVVDDQASVLPATSGQ"
     gene            9930..10006
                     /locus_tag="AC16QF_05260"
     tRNA            9930..10006
                     /locus_tag="AC16QF_05260"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9964..9966,aa:Arg,seq:ccg)
     gene            complement(10033..10530)
                     /locus_tag="AC16QF_05265"
     CDS             complement(10033..10530)
                     /locus_tag="AC16QF_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6447995.1"
                     /translation="MSIVTRHLERRQRGRVGSPMKTILIALAAVVTLTGCDFIPFSGG
                     KLEGMLAEVPGDWTTIAQPDVIQLETQPSDPYSVKLWIVGKGPLLYVHAGANRAAWVE
                     HIEANANVRVLIDGNLYELVAERVTDQGEFDAFGDLYEAKYGNRPRNEDVDEAYLFRL
                     GARSG"
     gene            10676..11308
                     /locus_tag="AC16QF_05270"
     CDS             10676..11308
                     /locus_tag="AC16QF_05270"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6447996.1"
                     /translation="MQLYTVPLAPNPTKVMLYIAEREDLGTEMGIEQVVVNPVKGQQK
                     APEHLARNPFGTLPVLELDDGSYLVESLAIVQYLEDKFPHGALLTGTPEEKGKARDVE
                     RIVDLRVAGPMAGYVHATKSPLGLPPDPERAEQLSSSIQTPLDYLEGLLSDGRPLLLG
                     EGVSIADCTLQAALQFLRYVEADLLGERSKLREWDLRYRARPAAKRVLRW"
     gene            complement(11435..12256)
                     /locus_tag="AC16QF_05275"
     CDS             complement(11435..12256)
                     /locus_tag="AC16QF_05275"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6447997.1"
                     /translation="MGRLEGKRAVIFGASMDGNIGQVMAGRFRDEGAQVLVASRNAAA
                     SAAFAESIGGYSAACDITRRDDVFALASHAKDALGGVDVAVNSTGLAMGGPFLEFGED
                     DLDTMIALQFKGSFFFLQAMVGAMLDKPEPGGSIIQISSAVAHRSTTVDLGFDAYMGT
                     KAGIDHVVRCVANQFGRQGIRANSIAAGHTDTPMHHDAYGGDIPDWMKAAFSDATPMG
                     RYGTCDDVAAGAVWLASDECFMTGDALQINGGLTLRRNPLKEDLDRYEREWAGRG"
     gene            12435..13730
                     /locus_tag="AC16QF_05280"
     CDS             12435..13730
                     /locus_tag="AC16QF_05280"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6447998.1"
                     /translation="MPPVFELESPRFLGDEDIPFERYTSQEFFNREMERMWTRTWQWA
                     CREEHVADPGDYYVYEIGPHSVLIVRDALGEVRAYVNACLHRGTKFRSGGSDGAFSEI
                     RCPFHGWTWELDGSLKRVPYAWDAPHVSEREFSLPRVAVGLWGGFVFVNLDPDPPPFD
                     ECLAPIPEHLENFDLSKRFVELHIEKELNCNWKAAIEAFIENYHTQETHPQLLYGSDD
                     EGTQYDVFTDHVSRFFCAFGVPSPSIDPPPTEQELVDMMLVGDRSVLGDAVTVKPGET
                     ARIVMARVLRQTLGEKYAADLGRYTDTEMIDVSQYGLFPNMVLFPQLSLPMVYRFRPI
                     GENPDRTLFELLILRPAPDQGRPPAPARPHRLEERDSFTTVPGFDTSLGEVYDQDTGN
                     LRAQQEGFRASRKHGATLLNYQEVRVRHLHQTLDDYLQR"
     gene            complement(13738..15183)
                     /locus_tag="AC16QF_05285"
     CDS             complement(13738..15183)
                     /locus_tag="AC16QF_05285"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006720 - isoprenoid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6447999.1"
                     /translation="MSTVMRGANEPPTCHGGLENLDEEHDYWIDDIDGTIPADLTGTF
                     MRNGPGRQKIGGKPFGHWFDGDGMISQFTFTDSKAHFRNRYVRTPKYVEETEAQEILY
                     RGFGTQIPGGWTKNIFKWPANPANTSIIHHGGRLLALNEGGRPWELEPGSLETVGEFD
                     YDGALAGKAFSAHGKVHPQTGDYFNFGAGTTGTVRKSPCLNMYRINPQGRMAKTAQLP
                     LQTFPFCHDFALAGRHAVYFINSIVFGGMGGFFLGRHSMSDGVRFDDDIPMRIIVVDL
                     DTLEVVREFETDPGAIIHFGNAFESGDEVVVDAMFQDNFEVNATLADVFNPSGRFGGG
                     YYNRYRLNLADGTMSCARVSECEAEFPVFNPSKAGQPHSVTWTACSVENGANSFFNGF
                     QRVTFDGDADVVTLPAGCYGSEPMFAAAEGATREDDGYLLEVVYDGYSHRSELQVYRA
                     DDVNERVCTLHLSHHLPHQFHGYFSPRTFLA"
     gene            complement(15185..16177)
                     /locus_tag="AC16QF_05290"
     CDS             complement(15185..16177)
                     /locus_tag="AC16QF_05290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6448000.1"
                     /translation="MVASRPEVEVEPGNFELVEEPVSGLEPGTLLLKTRYLTVNPPMR
                     MALVSGGIAGKPIPIGRTMYGSGLAEVVESRNPAFAAGDLVMGDLGWQEYVVSDGARR
                     RGVRKVAVPERLSPTTLMHVLGGSGHTAYFGMIEFCKPRVGDTLVVSTAAGSVGALVC
                     QLGRLQGCRVIGIAGSDEKCEWVVDELGAEACINYRTEDVAARLAETCPNGIDVYFDN
                     VGGETLDAVLGQIAMGARVVLCGGTSQYNNDLDWYGPKNYFNLVYKQAEMSGYFVSNF
                     AHRFDESLARLTTLVRDGRLKYREDVLEGIESVPSALIRILRGENFGIPLIKVS"
     gene            complement(16261..16713)
                     /locus_tag="AC16QF_05295"
     CDS             complement(16261..16713)
                     /locus_tag="AC16QF_05295"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR6448001.1"
                     /translation="MSNHTACVEWTMGEGDFVSGRYSREHRWTFDGGAVVQASPTPAV
                     VPEPFSNPACVDPEEAFVAAVASCHMLTFLHIAGRAGFSVIGYRDEAVGTMTKNAQGA
                     YWISEITLNPAIEYAGNAPTEAELDDLHDQAHEHCFIANSIRTEVLVG"
     gene            complement(16777..17466)
                     /locus_tag="AC16QF_05300"
     CDS             complement(16777..17466)
                     /locus_tag="AC16QF_05300"
                     /inference="COORDINATES: protein motif:HMM:NF016705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOUL family heme-binding protein"
                     /protein_id="HIR6448002.1"
                     /translation="MLKVLMIAAGVLVAVVVAGTVAWWYWGTTLEQPEYAVISKGDGY
                     EVRRYEGYLVAQTRVSGEFDQAGTRAFPVLAGYIFGNNVERREMAMTTPVVSYDADTS
                     SGSTMAMTAPVFSEAAGAEDEASVDPSQEKLWTYQFVMERKYSEASLPAPVDPRVEIK
                     RIPPRLVAARRFSGRWLEENVKAAETALLSSVRAGGLEPVGQVVLARYNDPFTPWFLR
                     RNEVLVEIAGD"
     gene            17667..18431
                     /locus_tag="AC16QF_05305"
     CDS             17667..18431
                     /locus_tag="AC16QF_05305"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6448003.1"
                     /translation="MSNPTQPLKLYGVPFSQPVRAVMWLMLHKRMPFEMVLTNPGTSG
                     ENGSRHPDYLAKNPGGTIPTIEEPDTGFTLGEAHAILCYLCNKYGWSDMYPAEPQERA
                     RVDWYLHYHHRNVREASTGLVAPKIRKDLNIPETVQAAAQATLTRALQAIETGWLGKS
                     AFLAGDAVTIADFAAYVEIGQLQPGFTNVYDFNPFPNVRRWMDSLKTFDSHDDVHVVL
                     AELGDISAEPPSMEAIKNANISALRTLKARLAEMSG"
     gene            18466..18894
                     /locus_tag="AC16QF_05310"
     CDS             18466..18894
                     /locus_tag="AC16QF_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007947151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6448004.1"
                     /translation="MSETHKGACFCGAVQIEVTGEPNMMGYCHCTDCAAWAGAPVNAF
                     SLWPPENVQVTAGEEKLGTYAKTEGSQRKFCTQCGGHVFTDHPGMGLIDVYLNVVPGK
                     AHTGAMHVFYGEKTMSVPDGLPKYRDLPKEFGGSGETIAE"
     gene            complement(18962..19648)
                     /locus_tag="AC16QF_05315"
     CDS             complement(18962..19648)
                     /locus_tag="AC16QF_05315"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6448005.1"
                     /translation="MSDDLLDNPAFAALQHAHAGLGRASELAAVYDPLVSPLAGLSEA
                     TPEALLDLAGLVPSGAGVGVLSTQPRLPESAGWRLLRVMPLHQMVCETPPGQPEPFDE
                     LTPSDAPEMVALAELTEPGPFLQRTIEMGRYVGVRSGGRLVAMAGERMKLPGCTEVSG
                     VCTHPDARGRGLALRMVLNVLAECFARGDRAFLHVVAGSPSETTAIGIYERLGFEHRR
                     PIYAHVLLRE"
     gene            complement(19794..20717)
                     /locus_tag="AC16QF_05320"
     CDS             complement(19794..20717)
                     /locus_tag="AC16QF_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6448006.1"
                     /translation="MHRALGVDQLGCTLFVLVWAFIGANPWEENHMAFEAIRYEKTDG
                     VATITKNNPPQYGMTLAVLTEMRAALIDANNDADVCVVVITAGGEGFHMGAVVFGEAR
                     SDWKFSPLEFREVIQVGHDLFRLIETLEKPVVGVAKAGAVGGGFENLHACDFVIASEA
                     ARFSQPEATLGMICGWGGTQRIPRMVGWRKAKEILMEGVELTGREAAEIGLITRAVPL
                     DEVDEAVSALCERLKICGPVALGYTKLAMNKVWETDYRSGLDYEVEASGMVNSAGEFT
                     AEVFEDFLAGRQPKFAKRQRLTAGREWRGTD"
     gene            complement(20736..21110)
                     /locus_tag="AC16QF_05325"
     CDS             complement(20736..21110)
                     /locus_tag="AC16QF_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6448007.1"
                     /translation="MPDLAIGYVNVFVSDFDTALAFYRDKLGLEVAMADEEFGYASFD
                     TKGVGFAIAAVGQDAEPAFAPGRHTGIGWGVESLDDAYETLRSTGVEFTAPPERQPWG
                     GYMALFEDPDGNVFYLDELREH"
     gene            complement(21231..22085)
                     /locus_tag="AC16QF_05330"
     CDS             complement(21231..22085)
                     /locus_tag="AC16QF_05330"
                     /inference="COORDINATES: protein motif:HMM:NF025679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HIR6448008.1"
                     /translation="MTVGLTDAPVDGVMAVHVEFRGITLKPQSGPAIDFALSEKVQLL
                     DLQNGSVFPLLDAQTVDAGRYNWIELHTNAEIDGILDSYVVESKSNGMVELEVPSGTV
                     RLVSGFVVTAGKSNAVLLDWDVRKGLTDPEGLVSPSPDAKDAYKLTPALRLIDMTQYG
                     SLTGSVADALIMAESCTSNAEGDGNLVYVYRNHDTTPDDLGSFGEPVTTAPVRSNPEK
                     AGAYTYTVHYLDPGDYTVAFTCQGLDDDPATDAAEGAAVPDIEFSGQVNASVAEGEGT
                     TNEAPPID"
     gene            22498..23028
                     /locus_tag="AC16QF_05335"
     CDS             22498..23028
                     /locus_tag="AC16QF_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448009.1"
                     /translation="MAGNLGLIAGLILVGVQITDSNRIASAELFGSSVGSVISINTEQ
                     LGDSPGEAMSRVLYDPAAAETSDLYIADRIYDALLRQLVRVHVFDTMGLYGDDELVSP
                     AGFVGLHFEFFACPYGLAWLDQQIELFDTRGNEELAILTSLKMLRELAGAESARVRLE
                     DRLERVGALLAQLDDS"
     gene            23176..24405
                     /locus_tag="AC16QF_05340"
     CDS             23176..24405
                     /locus_tag="AC16QF_05340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450 family protein"
                     /protein_id="HIR6448010.1"
                     /translation="MIDLNRPVNLASPEFAKDKYEWYERIREERPVFQTKISVLTVYT
                     VSRYEDCTNVLKDPNVLRNRTTATGGSRFPFPMPKSVQPLVQSMIQEDDPNHRRLREL
                     VRRAFRPQAIEQLESQIDAYSHELLDGLARRDSFDLQADYARPIPIRMIGDMMGLSRE
                     AVPQFQKAIRVLSEGFNGLKVLRTLFLDLPGAVRFVRALLDEKRSMPGDDVLTGLIEA
                     EDDGDRLTDDELVAMVFLLIVGGFETTLHLITNGVVTLLEHPEQLQRLKEDPGLIDSA
                     VEEILRHRGPVHGTKPGYAREDITLHGMTIPKGKPIMPLLAAANHDPRVFEKPQTFDI
                     ARSPNRHLGFGHGVHFCLGAHLARAEARVALRNLFERFPDLQLAVDPASLKLQAMPGW
                     HRYDGLPVIAGKMRSAA"
     gene            complement(24412..26043)
                     /locus_tag="AC16QF_05345"
     CDS             complement(24412..26043)
                     /locus_tag="AC16QF_05345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR6448011.1"
                     /translation="MAQAYDYIIVGAGSAGCVLAYRLSADPGVNVLLLEAGGVDRSPL
                     IGIPKGIGKLVRSPDHAWFFPVEQPRQPDLPASEVWVRGKVVGGSSSINGMIYSRGHP
                     EDYEEWMALGGPAWGWAEMKAAYRSIEDHELGSADHRGAGGLVHVSTGKLRYPAAEAM
                     IEAGREMGLERKDDLNDEMLEGVGYYAHNIRRGRRQSAARTFLKAARRRPNLHVQSQA
                     HVDRVCFDGNRAVAVACRVAGDRRVFEARGEIILSAGTILSPKILQLSGIGPADHLRD
                     HGIEVVRDVPDVGARMREHLGFSMPHKLKGTKGLNHRLRGLGLVGSALQYVATRGGPL
                     ATGPFEVGAFVRAVPGANRPDTQLYLGAFNYARSNDNFPVQLASPDSKPGMTIYGQLL
                     NLTSEGTVRLRSADPDDPPVIVPNWLSTDYDRAAAVAMVRYMRRYVRQPALARYVGDE
                     LIPGPTCQTDEEILNAVRRLSTSGLHGVATCRMGRDEGAVVDGRLRVRGVEGLRVADC
                     SVMPSLVSGNTNAPAMALGWRASDLIEEDAAGSRR"
     gene            complement(26045..26635)
                     /locus_tag="AC16QF_05350"
     CDS             complement(26045..26635)
                     /locus_tag="AC16QF_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR6448012.1"
                     /translation="MSKTVEIFFDFLSPPSYLAWTQVPGIVQRTGASAVWRPMFTIGL
                     HQLTENRSPVMVPNKARWIAGDLQRFARRYGVTLNPNPHGIINILPADRAAALAEKEG
                     AVEQLMATAYPAMWVDGRDLSDPEVLGEVIAAAGLDASRYLAAIETDEVKNLLKQNTQ
                     EAADRGAFGAPTFFVGDEMFFGQDRLDFVEEALAGG"
     gene            complement(26657..27388)
                     /locus_tag="AC16QF_05355"
     CDS             complement(26657..27388)
                     /locus_tag="AC16QF_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003210139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448013.1"
                     /translation="MTTSEKVVLVIGAGVATGGAVARRFAREGFIACMARRQGEKLQG
                     LVDEIAAAGGRAHGYSCDATEEAQVVDLVERVEAEVGPIEVACYNAAVGASHSITEFP
                     ADAYERVWRINTFGAFVMGREVSRCMVERGRGTILFTGATSALRGKARLAAFAGSKHA
                     LRALSESMARELFPQNIHVAHVIIDGPIDTPLIRRVMPSVFEERPADGVLLPDDIAET
                     YWNIHCQPRSAWLHETQLRPWLEPW"
     gene            complement(27460..28584)
                     /locus_tag="AC16QF_05360"
     CDS             complement(27460..28584)
                     /locus_tag="AC16QF_05360"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6448014.1"
                     /translation="MASLAWARRGETEQDRRLAPPLVEALAASRLCRLLLPGSLEGAG
                     VTPPAFLDVLAILSASEASLSWMVWNNALPCLLARYLQRSARAEIFADPGWLYASSTR
                     PSGRAVREEHGYRVSGRWALVSGCEHAEWIAFLLIVEEGGETSMLTPEVPEMRMAFLR
                     RGAYEIEDTWHTGGLRGTGSHHVRVTGQLVPERFTFSLADPPGDAHPLERLPVFCNMS
                     AGFAAQMLGIAGMARETLVTLARTKTTGSRMPDLRDRSAAQSFAAAQSAAGEAARSYL
                     YASTEQVWEDAVAGRTGDPAHVAALFAAAQHAIAIARDTVDGAFALVGTAALYVDNPL
                     ERAHRDMHAMLRHILAQPSWLEQAGRLEFGLEITEPMFFV"
     gene            complement(28676..29347)
                     /locus_tag="AC16QF_05365"
     CDS             complement(28676..29347)
                     /locus_tag="AC16QF_05365"
                     /inference="COORDINATES: protein motif:HMM:NF018961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HIR6448015.1"
                     /translation="MNTFEAIEDLLARCERLQDVAVDSLDEVCSSLGVQLARRFLSER
                     KHLYRRYLAFCLEDKELTEAEHADLAHLQHLLYLGAADVALIHDDVAHEVYGKALDEV
                     LSDLKISAEEEAFLRRLRSELKLSEAEAAVMLEAGRERMRDVALEQASTSDPEFSKPR
                     APIGQFVGRSDESLEQAVRDAVSKARLAIPRLHWFEVGNISGYVDDEGPKSWHVTVRG
                     GIKTS"
     gene            29524..30363
                     /locus_tag="AC16QF_05370"
     CDS             29524..30363
                     /locus_tag="AC16QF_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6448016.1"
                     /translation="MTTGRPVAVVIGATSKWQADGRNTRLAHGHDVDDSDLPVSARWG
                     IGGAVAQKFAREGFLVVLTTRTAANAAGLEAAIAEQGGECMTVELDLVSERSVSEAFA
                     TIRREAGEPEVVVYNAGYLEGRDLPPDQELLEHIPVEMFDTAQHISSRGPFLVAKEIL
                     PGMRERGSGSFLITNNAASLRGRKRYTGQSLYYPRVMMRTLAQVLTEEYSEHGVHVAN
                     IIVDGLIDSPGTRALPMAQQRPEIIMNPAAIADAYYYLHTQDRSCWTHELQLTPHSTK
                     PSF"
     gene            complement(30403..31206)
                     /locus_tag="AC16QF_05375"
     CDS             complement(30403..31206)
                     /locus_tag="AC16QF_05375"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013692821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448017.1"
                     /translation="MTANGLLENKIAVVVGGGQTPGETIGNGRATAIQFAREGARVLV
                     GDRDVGSAEETVAMILDEGGEAASAEIDVTVEESVASFMAAAVDRWGRIDVLHNNVGV
                     GLAGADAGVEEISADVFDQLVRINLRGMVLTAKHVLPGMRERGSGVIINISSMAAWKP
                     YPFVGYKTTKAGVIALTEQLAQANARYGIRANVILPGLMNTPMAVEPRVSQTMTREEV
                     IADRDRRVPLGRKMGTAWDVAHAAAFLASDRARFITGVALPVDGGASVS"
     gene            complement(31249..31854)
                     /locus_tag="AC16QF_05380"
     CDS             complement(31249..31854)
                     /locus_tag="AC16QF_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448018.1"
                     /translation="MSEALSPSPRPTLAGWIIRDVLITSGLAAMLLGLAAWHEAEGSL
                     AAALVAPALGFVAAYVLCYVYHEWGHLIGARLTGAAMPLAPYAGALIGHFDIQAHSRR
                     QFLWLSWGGVIAYVALMSIALSVYLHGGLGPAGAGFAVGGLAFVVQSLSVDVPQIVRV
                     MRGDDIARTSAEGANARVILRRTWQSWLVLAGALIVWNFAS"
     gene            complement(31864..33033)
                     /locus_tag="AC16QF_05385"
     CDS             complement(31864..33033)
                     /locus_tag="AC16QF_05385"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6448019.1"
                     /translation="MTWSPFAPEVMADPANGHRELLDRCPVHHCEDFDPPFFTLSRYA
                     DVEEALRDIETFSSHYGQGPRFTEPQGMLCDPPQHTYMRKLVQQAFTPKAMEALRIQV
                     ADLTEKLIDDILAGEPEFDLHDDFAFPLPVIIIAGMLGVPTEDLDRFKHWSDVQVAAM
                     GSSDPTRFEADQAAFFEYMVNHYRKRHAAIVAGEAVPDDLLTMIAGARADDGSFIAEA
                     DAQSMLTQLLVGGNETTTSLITNMVWRLLEDRERWQEVVADPVLVEAAVEESLRFDPP
                     VLGLYRNTTRDVVLHDTVIPTGSKVYINYAAANRDASVFERPDEFDLHREAKRHMSFG
                     LGVHFCLGAPMARLEAEIALKALARRMPDLKLLGPGQRIAPFFLWGRRNLPVAWR"
     gene            33182..35311
                     /locus_tag="AC16QF_05390"
     CDS             33182..35311
                     /locus_tag="AC16QF_05390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR6448020.1"
                     /translation="METAKSYCRYCHAYCAIDVDVEDGRPVAIRGDAKDPVYGGYTCI
                     KGRQLPELYDAPDRLHTALKKMPDGRFEEIPTARALDEIAARIERIIERDGPRAVASY
                     CGTYAFMESAALALARSFHQGIGSESFYTSVTIDQPGRPIAMSRFGTWGGGTHSFADA
                     DVVMSIGNNAVVSSFSVFGGIPPFNPYRRMNDAIKDGKKLIVIDPRRTEVARKAHLFL
                     QVRPGEDPTLLAGIIRVILDEDLHDQPFCDQYVDGLDAIKAAVDPFTLEYVARRAAVP
                     AEQIAEAARTFAAGPKGVAVTGTGPNMAERAPLTEHLVLALNTICGRVNRAGERLPNP
                     GILSPLTAVKAEVIPPAPAWGHGPRNRVRGLGQIFGEMPTAALSDEILEPGEGQVRAL
                     LSIGGNPIVAWPDQIKTKRALDALDLLVCVDIRLSATAQLADYVIPGKICLEREDMPV
                     LTDNWYDVPYGHYTEAVCQPNGDLREEWEFYWELAHRLNSDLPLPGGSLPTQEKPGKQ
                     TVLEHMFPNPKVPIADIRAQDGGHVFEGVQVMVEPGQGDARLQLTPGGVADELAEVRA
                     ETLQENGAPGKDGDYSHLLISRRLKHVYNSSGQQLPEIRKKGTTNPAYMNPEDVAALG
                     IGDGDVVQISSRHGQILGVAAAAPDVKRGVISMAHAWGGLPGNDGEVREIGASTNRLL
                     SNDKEYDPITGMARQSAIPVNVRPVAL"
     gene            complement(35335..36654)
                     /locus_tag="AC16QF_05395"
     CDS             complement(35335..36654)
                     /locus_tag="AC16QF_05395"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6448021.1"
                     /translation="METKKPNHELSPMRCPVHLEDVDLFSPGAAEHWYEAYEILHHEA
                     PVHRIPGEGFLPGTDGFILSKYEDIALVVREEERFPPAQTLIARQLLDKHIEPFDTPR
                     VNAAMGGVLVLRPTPETWRAHRQELTDPWVGPGAGRNEEMIRRTADELIDRWIDRGTV
                     DFVSEFAQPLPQMVMANILGWPLEDLKLLKYYGDGTVKPFVFGKGHRSVLPEEEVKSQ
                     FEVLEEFKTYTIALIEEKRRNPADDMITFLTEVEYSPVGRKLNDNEINSVVYNMVIGG
                     LETTQYALSEQVQLLIERDGVWDEIKADRSKVRGFTEDAMRLRAPTQGLSTRTTSRDE
                     VFQGVHVPKGSLLHLRWAAGNIDPDEWECPMDLKLDRRAGTRHLTFSQGARVCPGASL
                     SRLEQTVSWNALLDKVDRFAYGEDNNFMHQPGIMLGLEYLNLTFGKA"
BASE COUNT         6653 a        12425 c        11706 g         6212 t
ORIGIN      
        1 ggcaccccgt cgtgctggca gagcagatcg ccaccctggc gagcatcgcg ccggggcgct
       61 tcatcatgca gtgcgggctg ggcgacgacc ggcagggcaa ggccatgggg gtggacatgt
      121 cccgacgcgt gggcatgttt cgcgcgagcc tcgaagtcat gcgcgcccta tggcgtgggg
      181 agagcgtcga cgaggagcgc tactggcacc tctgcggcgc ccgcatcgcc ccgctgccgc
      241 cggagcccat agaggtctgg atcggcgctc tggtgcagcc tgccatacgg cgcgccgccc
      301 agcttggcga aggatggctg gcctcgccaa acctcacccc cgcccagacc gctgacgcgg
      361 caaacgtcta ccggcaggcc tgcgcggagt tcggcagatc gcctgccgcc gtcgccctgc
      421 gccgagacat attcgtcggc gcgaccagcc aggccgcgcg cgccaccgtc accccttacg
      481 tggaggccgg ctatcggggc atgagcgagg acgccctgct gttcggctct cctggcgagg
      541 tggccgatca gctgaggtcc tttgccgatc agggatacac cgacgtcatc gtgcgcaacc
      601 tcagcgcccg gcaggacgaa agcctggcca ccatcgagcg tctggccgag gtgaaggctc
      661 tgctgaccgg ctgacgcccg aagagcacga ccgacccgag aggacgtttc aatgtacaga
      721 cttcacggcg ccaccagctc accgttcgtg cgcaagacgt ggatcttcat gaaggaaaaa
      781 gggctcgagt tcgagcattg cgagctagac cctctggaca agtcccaacg ctttctagcc
      841 atgaaccctc tgggccggat cccggtgctg gaggagcccg atgggaactt catcgccgac
      901 tcgtccgtga tctgcgacta cctggagcgg tgccatcccg ggcccgcgct ctacccctcg
      961 aacaaccgcg accgcgcgcg ggcgctgtgg ctcgaggagt atgccgacac ggcgctgaac
     1021 caggtgtgcg cgcggatttt ctggatgcac atcatcattc ctttccgtac cggcaggccg
     1081 gcagaccagg gcgagatcga cgctttcaag gaagaggatt acccgggggt gttcgactat
     1141 ctggaccggg cagcgccgga gacgggcgac gggatggtgg gcggccggtt cggcatcgcc
     1201 gacatggcgc tggccgcccc cgtgcggctg ctcgatctcg ccggtgaccc gctggatgcc
     1261 acgcgctggc cccgtttcga cgcgcactat cagcgcgtcg ttgcgcggcc ctcggcgcaa
     1321 tccatcgtcg ccaaggatct ggccaccacc gaggtgtggc ggaccaccgg caacgcgccc
     1381 gcctccacgc agagcggcta acggggagcg agcgcctgga gatgccggtg gggtggaaac
     1441 cccacgacac ggcgaaagcc ggtcagcccg tcggagaacg tcgtcgagcc cgccaggtca
     1501 tcgagcctgg ccaccaggtc ctcgagatcg tcctcgttgc gccggcaaca ggccagatag
     1561 gccgcaagca ccccgacgag cgaggcgccc ttgcgcttca agcgctcctc catctcaaga
     1621 aagtagctgg cgccacccca gtaaatggtc gggtagagcc cctcggcacg cagctgggga
     1681 accgcctcga cgaaccggcg gttcggctgg ctgtaggcgg cggcagccgt gtcgagacgt
     1741 tccgagtagc gcgcagccat cgcctcgtcg tccagcacgt ccatgatccg catgacgcgg
     1801 aactgcatga agctggcgaa gccctcggaa aaccaggcat gctcgcggcc gacgaaaggc
     1861 agaaccagat ggctgagctc gtgaggcgcg gtccagtcat tgcgaaacgc ctcgaccggc
     1921 tgtgtcgggt ccacatacaa gcgcacgccc tgacggcggc cgcgcagggt gttggcccag
     1981 ggaacggggc ccgccccctc gtgacgggca aatcgaaaac gcacgtccat gggcaaaggc
     2041 ccgacgagaa cctccagcgc accggccacc tcggcaatcc aggcttccag cttgcggcgc
     2101 tccgattccg taaagccgtc gtcgtagaca aactcgagac ccgcatcccg gggcaccggc
     2161 ttctcatccg cctgggccat gctgcagacg cctatcccgt tgagcaacac caagcccagc
     2221 actcgcaaac gccagcgaga cgtcatcatc gcgtggggct ggccggcgcg tcgaaataac
     2281 ccagcgcttc cgcggccaat cgccgcccga gaatggcgtc tgccgccttt tcagcggtca
     2341 tgatcgtagg ggcgttgagg ttgccgttgg tgatgtgagg gaagatcgag gcgtccacga
     2401 cccgcagccc ctggattccg cgaacgcggc actccgagtc caccaccgcc attgggtcgt
     2461 tccccatgcg gcaggttccg caggggtgat aggcactctc cgtgctgcgg cgcacgaagg
     2521 catctattgc cccgtccgaa tcggcgccgc tccccggggc gatctcgaca ccccgataag
     2581 ggtcgaatgc ccgctgggcg aagagctccc gggagaggcg aatcgcccgc cggaacacct
     2641 gccagtcttc ctcgcaggac atgtagttga aactgatgac gggggcgacg tccgggtctg
     2701 ccgagcgcag ggtgaggcgg ccgcggctgg ggctcagcat gggccccacg tgcacctgaa
     2761 aaccgtccgc atcggccacc gaccgcccgt cgtaggacac ggccgccggc agaaagtgaa
     2821 actggacgtc cggccagggg acgtcggctg cgctgcgcac gaacccgccg gtctcgaaat
     2881 ggttggaggc ccccggaccg gaagcggtga gcagccaccg agcgccgatc acccccttgc
     2941 ccaacgggtt gagccagcgg ttgagcgaca cgggctgagt gcacgcctgc tgaaaataga
     3001 tctccagatg gtccatgagg ttggcaccca ccccggggag ctcgtgacgc acgggaatcc
     3061 cgtgaggttc gagggcttcc ccgtgtccga tgccggagag catgagcagc tgaggagagc
     3121 cgatggctcc tgccgccaga accacctcct cccgggccac cgcggtgtgg gttgcccccc
     3181 gatgccggta ggtcaagcct tcgcagcggc ccgattccac gttcagccgc tgaaccgggg
     3241 agcccgagcg gaccgtcagg ttgctccgcg agacagcagg ggcgagatag gccagggccg
     3301 cgctgcagcg aaccccgtcg cgcaccgtca tctccaggtc accaaagcct tcctgccggt
     3361 aaccgttgag gtcgtcgctg agcgcatgcc ccgcttgctc agccgcctgg agaaaggtct
     3421 cgtagagggg attggcgcgc cgcccgcggc tgacggtcag cggtcccgcc cggccccgat
     3481 aggggtctcc ctcggcagcg ccgagcacat gctcggcccg cttgaagtag ggcaggacgt
     3541 ccgcgtagcc ccatcccgta gcacccaggc tctcccagcg ctcgaagtcg cgcgggtggc
     3601 cgcgcacgta aaccatgcca ttgatggacg acgacccgcc cagcacccgc ccccgcgggc
     3661 aggccaggct tctgccgtcg agcgccggct cggcctccgc ctgatagcac cagtcgaatc
     3721 gcggccggcc aatgggcagc gagaacgccg aaggcatgcg aacgaacaga tgccgatcgc
     3781 tgccaccggc ctctaaaagc aacaccgatc gggcaccgtc ctcggagagc cggttggcga
     3841 gcacgcatcc cgcggagccg gcccctgcaa tgatgtagtc gaagacttcc aacgatcccg
     3901 taccctgccc agtcactgct gcgcgtctgc ccgaggtgca ggctagcaga cgccgggcgg
     3961 aaatggggca cagcggcgcg gcggcggtta gaattccagg tcagcgcccc ggggaaggaa
     4021 gatggtgagc ggtagcctgc tggccggatg gttcgcgccg ctggcggttt atcttgcgat
     4081 actgatcctg catctcgccc tgcccgcgcg gagggtagac gggtacgcgc tggatcccgg
     4141 cacgggccag cctctgacct accgcctgaa cggccttgtc gtgttcgccc tcgtcacttc
     4201 ggtctggctc gctgcaggct ggaagggctg gatcagctgg gactggctct acgtcaaccg
     4261 ctggagcgca atgggcgggg cctgtctggt cgggctggcc tacagcctga tcaccgtgct
     4321 cggcgcgccc ccgaccggcc ggtcactttt agccgatctc tacctcggca ggccggaaaa
     4381 tccgcagcac ttcaacaagc gtgtcgatgc gaagatgttt ctctatctcg cgggcgccgt
     4441 tctgctcggc ctgaacaccc tgtccgccac ggcgtttcac gcgggtgccc tgggtagcgc
     4501 cagcaacccc ggcgtatatc tccatgcagc tctgctcact tttttcgtgc tggactatct
     4561 cgtcttcgag cgggttcatc tctacaccta cgacctgttc gccgagcgcg tggggctcaa
     4621 gctcggctgg ggctgcctgg ccttctaccc cttcttctac cctgtgggcc tgtggggcac
     4681 cgcccacctg ggcaagccgt cggtgatcgc cgacctcggc tggatctggc tcgcgctgag
     4741 cgcgctcgtt tttctgtccg gctgggtact ggcgcggggc gcaaaccttc agaagtacgt
     4801 tttcaaaaga ttcccggagc gggcgttcct gggccgattt cctccgcggg tcattcacgg
     4861 cggcaacctg gccctgctgt gcggcgggtt ctggggcgtc gcccgacacg tcaactacct
     4921 gggcgagatc ctcatggcca cgggtctcgc gatgtccctc gggcatctgg acagcccctg
     4981 gccctggcta tatcccctgt actatctggc cctgctggtg ccgcgggagc gcgatgacga
     5041 ccgtcgctgt gcggagaagt acgggacgct gtgggacgag taccggcgcc gggttcccta
     5101 ccggatcgtc ccgcggatct attgacgcag cggagcacgg acctgcacat gaatctggat
     5161 ctcatacttg ccctgaaggt gctcgcgctg gcgctgagcg ccaatggggc tccggtgatc
     5221 ggcaagcgcc tgttcggcgc ccgctgggcg atccccctgg acggcggtct cacctttctg
     5281 gacgggcggc ctctgctggg gtcgagcaaa accgtccggg ggctggtgct gagcctggcc
     5341 accaccaccc tcgtggcaac ggcgctaggc tacggctggc agctgggtgt gatctactcg
     5401 gcgttgacga tgtccgggga cgcgctgtcg agcttcgtca agcggcgccg gggcattccg
     5461 cccagcggtc ggttccgcgg cctggatcag atccccgaaa ccgctctgcc catgtgggtc
     5521 tgcagctcgg cgctggggct gacgggctgg gacatcgcgg tgctcgtggc gttgttcttc
     5581 gccggtgatt tcgtgctgtc gaaagtactc taccggatcg gcatccgcga cgagccctac
     5641 tgaagctgcg cggcgccagg gcccgccgaa tgaaagacgt gcaccgtaag ctctggcgga
     5701 cagttcaggc ggacggcgac catggatgcc cctgcgccgg gcgacgtgta cccccgcatg
     5761 cctttgtaat cccagcatcc gcgtccgagg cgcctgggaa cgcgagagtc gagcgtgacc
     5821 ggatagcccc ccggcaggca gatctgaccc ccatgggtat ggccgcacaa cagcagctgc
     5881 acgccggcgc gcgccgcctg ccggtagacc tcgggcgtat ggctcagcag gatcctgggc
     5941 accgtgtcgg gaacgccggc cagagccttc tcgatgtcgt gcacgccgaa gaagtgagca
     6001 tcgtcgatcc cggcaaggta gaggtgatcg tcaccgtgcc ggtgggcgtg actttcgttc
     6061 atgagcatgc gaatgcccat gtcctccagg gcgggcagca tggtcacgct gtcgtggtta
     6121 ccgaagacgg cgtagaccga tccgtccagg ttgctgcgca gccgggccat gccgcgcagg
     6181 gcatcgtcga tggcgccgta ggtgcggaaa cggtagtcgc cggtcagcac gcagacgtcg
     6241 taggtcagct cccggaccct ttcgatgatg gcgtgcagca gctcgtcgct cacgtccacg
     6301 tgcagatccg agaggtgcag gacccgcagc ccgtctagac cggcgggaag agcgtcgagc
     6361 accacgtgat tctccaccac ccgatgatcg agagcgttcc tgctggcgcg ggcatacagg
     6421 cccgagacct tgagcgagtt gcgaatgatg gaatgaaccg agtaccagtt ctccgggtga
     6481 aaaaagcgga tcccatggct gaatagccga gactcgtgag ccgtctccac gccgaggcgc
     6541 tcacgggcgt gggcggtgcc taggcgtttt tccaggcgtt gcagaatctc gctgtcgcgc
     6601 atctgaagat tatatatgcc acgcccctgc ccgctgacgt ttcccgctat ccccgatcgc
     6661 cgccgaactc gggggggcgt ttttccatga acgccctggc cccctcctgc atgttacgcg
     6721 ggatgagcac tccctggtgc gccatctcca gcccaagctg atccccaaag gtgtggtgaa
     6781 tggctgcatc aacgcattcg cgaatgcggg tgcacgcgct cggcgaggag cgcttcagaa
     6841 ccatcgccac gcggccgact tccgcgtcga gctcctcatc cggcaccgag cgccagatga
     6901 ggccccaggc ttcggcctgg ttcgcggtga tgcgatcgcc cagcagcgtc atccccaggg
     6961 cacgagcacg cccaaccctc atcggtatgc tccaggtgga gccgagatcg ggcacgatgc
     7021 cgagattggg gccgaaggtc gccacgaaga ttgccgactc cgcggcgatg gcgacgtcgc
     7081 aggccagcgc cagcccgagc ccgccgccgg ccgcaggacc attgacccga gcgatggtcg
     7141 gcaccgggca gctcatcagc gcgctgaccg cgccgttgaa gagatcgccg ctgtccgggg
     7201 cgtcctgcag gtccgggtct gccggcccct ccgccgcgcg ggcgagatcc gcgccggcgc
     7261 aaaagccgcg ccccgcgccc gtcaagatga ccacgcgcac gctttcgtct tcgacaaccc
     7321 gttccatggc ccggcagatg ccgcccatga gctcgtcgtt catggcgttg aggacctcgg
     7381 gccggttcag aatgagcgtg gctgtgccgt cgtcaatctc gcagaggacg atttctgagt
     7441 ctgacatagg actcccccat gaagagcgga tgaatcagct gcatcccatg gaaccagaaa
     7501 cgtcccggag cggacaaggg ctacgcgcca tcgcgccggg cggggaggtc aataacccgt
     7561 tggcggtctc agaaccggaa gttgaaaccg acgaggggcc cgtggaaccc gtactccgtg
     7621 gtcagctcgg catcgtcgaa ctcggtgtcc cgatagcgat acccgaacat gatccggttc
     7681 atgcgccgct tgcccaccgt gtaggcggcc agtgcctgag cctgccagct gccttcggat
     7741 tcgccgaagc ttacgtcccc gagggtgagg atggaccacc gatccgacag atcgaaacgg
     7801 tagcgaacgc ccagaagagc atcgaagtag tcggccgacg aatctcgacg ggcgacatcg
     7861 tcgccctgga attgcagacg gtaggtgttg tcgaacccgg tgtagcgcgc tccgccatag
     7921 atgctcagtg gcgagccggc gccgcccggc cagaaggcaa ccccggcgtc caggaacacc
     7981 tgcttgttct tggttttcag ccgcaacgca tcctcaccag tcgcgtccga gatcttgacg
     8041 aaggtcacat ccgcaaaggc gctccagcga ccccgccccc cttccacggc cacctggaac
     8101 gccgcatcga gattgcccag cgcgtccttg aaatcgatct cggtgccgcc gacgggcgag
     8161 tcccgccagc tgagatccag cttcgtatcc atggcccaga tgtagggcgt gatggaccac
     8221 caggtctcgt cgccttgttc cgcgcgaagc ccgctgctgc caagcatcag caatgcggcc
     8281 atcaggaaga tgaccgaatg ccgtcccacg ctgacccatc caagattcat aactgcaagt
     8341 cctctcgcta cgctcggcta gtcccaacaa cgtgccacgt acctgaaatc agcaggccct
     8401 cgacgacgcg catcatactg gacctgtgct gatgtggcat cattgacctc ttcgagaatg
     8461 aaaacgtcag ccatcaagag gtcgccaagc cttgattact gagcgagcca acccatcgct
     8521 gcgcttgttt gctgccgtgg ccatgttggc tgctctgacc gcatgcgctg caaaagggcc
     8581 ctacgtgctg gagctgatgc ccgcgcccac cgtttaccag gacgaggact tcagcccctt
     8641 cgccgacgac ttcagcccgg aatcgttgcc gtatcgcggc atgctgtacg taacggaccg
     8701 caggccggct ggtgccggaa agccgcccga tcccgcagac ccgcactacg tcggcaagcg
     8761 cggcgagggc cttcgggcag gcgtcgcctc catccagctc ggcgagggcg gcttcacctg
     8821 ggaagaagcc attcgggtgt cgatggcgag cgatcgcggc gagggatacc ggttgagcgt
     8881 gaccggcgtg gaagagctcg ggttcctcga gccgagcatc gagccattca tcgaagcgcc
     8941 cgatttcccg ctggcggacg aagcgttcgc ggcgctggtc aacgacaagc tcgcccagtc
     9001 cgagcagaag gacgtctaca tctacgtgca cggctaccgg gtgcggttcg aatacccggt
     9061 gctcgtggcc acggagctct ggcacttcct gggctacgaa ggggcattca tcgctttctc
     9121 gtggccttcc acgccgagct atctcgccta cttcgccgac gccgaggtgg cggtggtctc
     9181 cgcccgacag ctgcgcatcc tgctggaatt ccttgcgcag agcaccgacg tgaggcggat
     9241 ccacattctg ggctacagcc agggcacccg cacggtcatg caggccctgc acatgctggc
     9301 gctgaagtac gaagaggacg atcgcgagtc gctgctcgag cggctgcgta tcggcaacgt
     9361 ggccctcgtc ggcagcgaca tcgatcggga gacaatgggg atgtacgtag ccgacggcct
     9421 gatgcgggtg acggagcgta tgaccgtcta cgcgtcagcc aaagacaagg ctctgcgcat
     9481 ctcgcggcgc gtattgcggc gcgatcggat cgggcagctc attccctcgg acgagatcac
     9541 cgacgagctt cggggcttcc tcattaccac accggaattc gagatcatca acgtcaccga
     9601 tgccgaagac gccaccaccg agaacggcca ccgttacttt cgaagcagcc cgtgggtgag
     9661 cagcgatctg ctcaccagct tccgctacgg aatgacgcca gggcaacgcg gtctcgagat
     9721 cctgccggag actccgatct ggcagtttcc ggccgattac ccgacgcgac tgcgctctgc
     9781 cctcaacgac gttgtcgacg accaggcatc ggtgttgccg gcaacgtccg gccagtagct
     9841 gcccagggcg atcacgtcac cagagacgtc cagcgcctgc cgccgggccc tgtcgatgtg
     9901 gtcggttgtg ccctataatc gccgcccccg cgcccgtagc tcagctggat agagtactgg
     9961 cctccgaagc caggggtcgc gcgttcgaat cgcgccgggc gcgccaatca ccgcgggtgc
    10021 tgggcaggca gatcagcctg atcgagcacc gagacgaaac aggtaagcct cgtcgacgtc
    10081 ctcgtttctc ggccggtttc cgtacttggc ctcgtacaga tccccaaagg cgtcgaactc
    10141 gccctggtcg gtcacgcgct cggccaccag ctcgtagaga tttccgtcga tgagaaccct
    10201 tacgttggcg ttcgcctcga tgtgctccac ccaggcggcc ctattcgccc cggcgtgcac
    10261 gtagagcagc gggcccttgc cgacgatcca tagcttgacc gaataggggt cgctgggctg
    10321 agtctcgagc tggatcacgt ctggctgcgc aatcgttgtc caatccccag gtacttccgc
    10381 caacatcccc tccagcttcc ctcccgagaa aggaatgaag tcgcagccgg tcaacgtcac
    10441 cacggccgcc aacgcgatca ggatcgtctt cattggggag ccgacgcgtc cccgctgccg
    10501 tctttcgaga tgccgggtga caatactcat ctcaccgtcc tcctgtattg attgccggag
    10561 atcgccgttc gcgatcattt caaaccattc gtgcctggac accgaacatc ctatggtggc
    10621 atgttcccgg gcccggcgca ggctgcgggg ccgagccgtc attggaggat ctttcatgca
    10681 gctttacacg gttcccctgg cccccaaccc gaccaaggtg atgctctaca tcgccgagcg
    10741 ggaggatctc ggcaccgaaa tgggcataga gcaggtcgtc gtcaacccgg tcaaaggtca
    10801 gcaaaaagcg ccagagcatc tcgcccgaaa tcccttcggc acgctgcccg tcctggagct
    10861 cgacgacggc tcgtacctcg tcgaatccct ggcgatcgtc cagtatctgg aggacaagtt
    10921 tccccacggg gccttgctga cgggaacccc cgaggagaag ggcaaagcgc gcgacgtcga
    10981 gcgcatcgtc gacctgcgtg tggccggccc catggcagga tacgtgcacg ccacgaaatc
    11041 cccgttgggc ctgccgccgg acccggaacg agccgagcag ctctcatcca gcattcagac
    11101 ccccctcgac tatctggagg gattgttgtc ggacggtcgt ccgctgctgc tgggagaggg
    11161 cgtcagcatc gccgactgca cgctgcaggc cgccctccag tttcttcgct atgtcgaagc
    11221 agacctgctg ggtgagcgtt cgaagctccg cgaatgggac ttacgctacc gagcccggcc
    11281 cgcggccaag cgcgtgcttc gctggtagca aacgccatga tgttccagac accccggctg
    11341 catttccgat tgttcgaggt ccaggacctc gaggagctgg cgagcatcaa cgccgaccag
    11401 gagacatccc gccacgtcgg cgacggcaga gcgctcagcc gcgcccggcc cactcacgct
    11461 cgtagcggtc aagatcttcc ttgagggggt tgcggcgcag ggtgaggccg ccgttgatct
    11521 gcagagcatc tcccgtcatg aagcactcgt cgctggcgag ccagacggcg ccggcggcaa
    11581 cgtcgtcaca ggtgccgtat cggcccatgg gggtcgcatc ggaaaacgcg gccttcatcc
    11641 agtccgggat gtcgccaccg taggcgtcat gatgcatggg cgtgtccgta tgaccggccg
    11701 ctatgctgtt cgcccttatg ccctggcgac cgaactgatt ggccacgcaa cggaccacgt
    11761 gatcgatccc ggccttagtg cccatgtagg catcaaaacc gagatcgacg gtcgtggagc
    11821 gatgagcaac tgcagagctg atctggatga tcgatccccc aggctccggc ttgtcgagca
    11881 tggcgcccac catggcctgc aggaagaaga acgagccttt gaactgcagc gcgatcatgg
    11941 tatccagatc gtcttcaccg aactcgagga acggcccgcc catagccagg ccggtggagt
    12001 tgacggccac gtcgacgcca cccagcgcgt ctttggcgtg gctggccaga gcgaagacgt
    12061 cgtcgcgtct ggtgatgtcg catgccgccg agtagccgcc gatggattcc gcgaatgcgg
    12121 ccgacgctgc ggcgttgcgg ctggccacca gcacctgggc accctcgtca cgaaaccgcc
    12181 cggccatcac ctggccgatg tttccatcca tcgatgcgcc gaagatcacg gcgcgcttgc
    12241 cttcgagtcg tcccatcgtc ccctccctgt ccaacactgg cgagctgacg ttcattgtac
    12301 aggggttcaa atgaccttag gctagccgag ccactggtcc gggtcgacag acgatggaag
    12361 aacccaagcc gaagccgctg gctcgttcgt ccacaccatc cgcgcaggag ctggtgcacc
    12421 gagacggacg cccggtgccg cctgttttcg agctggaaag cccccgattc ctgggcgacg
    12481 aagacattcc tttcgaacgt tacacgagcc aggaattctt caatcgggag atggagcgga
    12541 tgtggacccg aacgtggcaa tgggcctgcc gcgaagagca cgtcgccgac cccggcgact
    12601 actacgtgta cgagatcggt ccgcactcgg ttctgatcgt tcgggatgcg cttggggagg
    12661 tcagagccta cgtcaacgcg tgccttcacc gcggaaccaa gttcaggtca ggcggctccg
    12721 atggcgcttt cagcgagata cgctgccctt ttcacggctg gacctgggag ctggacggca
    12781 gcctgaagcg ggtgccctac gcctgggacg cgccccacgt cagcgagcgc gagttctcgc
    12841 tgccccgggt ggccgttggg ctgtggggcg ggttcgtctt cgtcaacctc gatcccgacc
    12901 cgccgccttt tgacgagtgt ctcgcgccga ttcccgagca tctcgagaac ttcgatctgt
    12961 cgaagcgttt cgtggagctg cacatcgaga aggagctcaa ctgcaactgg aaggccgcca
    13021 tagaggcctt catcgagaac taccacaccc aggagaccca cccccagctg ctctacggca
    13081 gcgacgacga gggcactcag tacgacgtct tcaccgacca cgtgtcccgc ttcttctgcg
    13141 ccttcggagt cccaagcccc agtatcgacc cgcccccgac ggagcaggag ctggtggaca
    13201 tgatgctggt cggtgaccgg tcggtgctcg gcgacgccgt gacggtaaag cccggagaaa
    13261 ccgctcgaat cgtcatggcc cgggtgctcc gtcagaccct gggcgagaaa tatgcagccg
    13321 acctcggccg ttacaccgac accgagatga tcgacgtctc tcagtacggc ctgtttccga
    13381 acatggtgct gtttccgcag ctgtcgctgc caatggtcta ccgattccgc cccatcggcg
    13441 agaacccgga ccggacgctg ttcgagctgc tgatcctccg gccggcaccc gaccagggtc
    13501 gcccaccggc tccggcgcgc ccccatcgcc ttgaggagcg cgacagcttc accacggtgc
    13561 ccggcttcga caccagctta ggcgaggtgt acgaccagga cacgggcaac ctccgcgctc
    13621 agcaagaagg gttcagggca tcacgcaagc acggcgcgac cctgctgaac taccaggaag
    13681 tccgggttcg ccacctgcac cagaccctcg acgactatct gcagcggtag aagccgtcta
    13741 ggcgagaaac gtccggggcg agaagtagcc gtgaaactga tggggcaggt gatggctaag
    13801 gtgcagggta cagacccgct cattgacgtc gtcggcccgg tagacctgca gctcgctgcg
    13861 gtgtgagtag ccgtcgtaaa ccacttccag aaggtagccg tcgtcttcgc gggtcgcgcc
    13921 ctctgcagcc gcgaacatgg gctccgagcc gtagcagccg gccggcaggg tgacgacgtc
    13981 tgcatcgcca tcgaaggtca ctctctgaaa accgttgaag aagctgttgg cgccgttttc
    14041 caccgagcag gccgtccagg tcaccgagtg cggctgcccg gctttcgacg ggttgaagac
    14101 ggggaactcg gcctcgcatt cactcacccg tgcgcagctc atggttccat cggccaggtt
    14161 caggcggtag cggttgtagt acccgccgcc gaaccttccc gacgggttga acacatctgc
    14221 cagcgtcgcg ttgacctcga agttatcctg gaacatggcg tccacgacca cctcgtcacc
    14281 gctttcgaac gcgttgccga agtgaatgat cgcgcccgga tccgtctcga actcgcgcac
    14341 cacctccagg gtgtcgagat caacgacgat gatgcgcatg gggatgtcgt catcgaaccg
    14401 cacgccgtcc gacatgctgt gtcttcccag gaagaaaccg cccatgccgc caaagacgat
    14461 ggagttgata aagtacacgg cgtggcgccc ggccagggcg aaatcgtggc agaacgggaa
    14521 cgtctgcagc ggaagctgcg ccgtctttgc catgcgaccc tggggattga tgcggtacat
    14581 attcaggcac ggtgatttcc tcacggtgcc cgtggtcccg gcgccgaagt tgaagtagtc
    14641 gcccgtctgc ggatgcacct tgccgtgggc gctgaacgcc ttgcccgcga gcgcgccgtc
    14701 gtagtcgaac tcgcccaccg tctctaaaga gcccggctcg agctcccacg ggcgaccgcc
    14761 ctcgttcagc gccagcagcc ggccaccgtg gtggatgatg ctggtgttcg cggggttggc
    14821 cggccacttg aagatgttct tggtccagcc tccggggatc tgcgtgccga atccccgata
    14881 gaggatctcc tgcgcttcgg tctcctcgac gtacttgggc gttctgacgt atcggttgcg
    14941 gaagtgggct ttggaatcgg taaacgtgaa ctggctgatc atgccgtcac cgtcgaacca
    15001 gtgaccaaag ggcttgccgc cgatcttctg acgccccgga ccattgcgca tgaacgtgcc
    15061 ggtgaggtcc gcaggaatcg ttccgtcgat gtcgtctatc cagtagtcgt gctcctcgtc
    15121 cagattctcc aggccgccgt gacaggtagg cggctcgttg gcgcctcgca ttaccgtgct
    15181 catgtcatga aaccttaatc agtgggatgc cgaaattctc gccgcgcaga atccggatga
    15241 gcgccgacgg aacgctctcg atgccctcga gaacgtcctc gcgatacttc agacgcccgt
    15301 cgcggacgag ggtggtcagc cgggcgagag actcatcgaa acggtgcgcg aagttggaga
    15361 cgaagtagcc ggacatctcc gcctgcttgt aaaccaggtt gaaatagttc ttggggccat
    15421 accagtcgag gtcgttgttg tactgcgacg tgccgccaca gaggaccacg cgggcgccca
    15481 tggcgatctg cccgagcacc gcatcgagcg tctcgccccc gacgttgtcg aagtagacgt
    15541 ctatgccgtt cgggcaggtt tcggccagac gcgcggcaac gtcttccgtg cggtagttga
    15601 tgcaggcctc ggcaccgagc tcgtcgacca cccactcgca cttctcgtcg ctgccggcaa
    15661 tgccgatcac ccgacagccc tgcaggcggc cgagctggca gacgagcgcg cccacgctgc
    15721 ccgccgcggt tgagaccact aaggtgtcgc ccacccgggg tttgcagaac tcgatcatcc
    15781 cgaagtaggc cgtgtgcccg cttcccccga gcacgtgcat gagggtcgta ggtgacagcc
    15841 gttcaggcac cgcgaccttc cgcaccccac gccggcgcgc gccgtcagag accacgtact
    15901 cctgccagcc gaggtctccc atgaccaggt ctcccgcggc aaaggccggg ttgcgagact
    15961 ccaccacctc ggccaggccg ctgccgtaca tggtccggcc aatggggatg ggcttcccgg
    16021 ctataccgcc ggaaaccaac gccatgcgca tgggcgggtt cacggtgagg tatcgggtct
    16081 tgagcagcag ggtgccgggc tccaacccag aaaccggttc ctccacgagt tcgaagttgc
    16141 ccggttccac ctcgacttcc ggccgagacg ccaccaccca ctttctgttg atcaggccgc
    16201 tgatgttcta ccccctctac tcgtccgtgc ccatcgtacc ggtagccgtc taatcacacg
    16261 tcagccgacg agcacctcgg tgcggatgga gttcgctatg aagcaatgct cgtgcgcctg
    16321 atcgtgcagg tcgtccagtt ccgcttccgt aggcgcgttg ccggcgtatt cgatcgccgg
    16381 attgagcgtg atctcgctga tccagtacgc accctgggcg ttcttggtca tggtgcccac
    16441 cgcctcgtcg cgatagccga tcaccgaaaa tcccgcacgg ccggcgatgt gcaggaaggt
    16501 aagcatgtgg cagctggcca ccgccgcgac gaaggcctct tccggatcta cgcaggcagg
    16561 attcgaaaac ggctccggca ccaccgccgg cgtgggcgat gcctgaacca ccgcaccgcc
    16621 atcaaacgtc cagcggtgct cgcgggaata gcggccgctg acgaagtccc cttcccccat
    16681 ggtccactcg acgcaggctg tgtggtttga catgacgttt ctccaaccag aggcaacaat
    16741 cctacgccga gacagacccc acgaccatgg cggctgtcaa tcgccagcga tctccacgag
    16801 cacctcgttg cgtcgcagaa accacggcgt gaaggggtcg ttatagcggg cgaggaccac
    16861 ttgccctacg ggctcgaggc cgccggcgcg caccgaactc agcagcgccg tctccgcagc
    16921 cttcacattc tcctcgagcc atcggcccga gaagcggcgc gccgccacca accgcggcgg
    16981 aatccgcttg atctcaacgc gcggatccac aggcgcgggc agtgacgcct cggagtactt
    17041 gcgttccatc acgaactggt acgtccacag cttctcttgc gatggatcaa ccgatgcctc
    17101 gtcttctgct ccggcagcct cgctgaaaac gggcgcggtc atggccattg tgctgcctga
    17161 agacgtgtcg gcatcgtagc tcaccaccgg cgtggtcatg gccatctccc gacgctccac
    17221 gttgttgccg aagatgtacc ccgctagcac ggggaacgcc cgggtacctg cctggtcgaa
    17281 ctccccggac acccgcgtct gcgcgacgag gtagccttcg tagcgacgaa cttcgtagcc
    17341 atcacctttc gagatcaccg cgtattcggg ctgttcaaga gtcgtgcccc agtaccacca
    17401 ggcaacggtc ccggccacga cgacggcaac gagcacgcct gctgcaatca tcaggacctt
    17461 caacatgatg ggagcttcgt tcgggcgacg agcatggcgg tcatggtctt tcctttctcc
    17521 tcgaacctgt attcaaccat gctcgcgaga cggtgaaatg cacgtgaatc ccccgcgccg
    17581 gaatgcgacg ggactgcggt gcagcctcgg gagcttatca ccggacagaa ggcatgatag
    17641 cttggccgtc cgatcaggga ggaatgatga gcaaccccac ccaaccactg aaactctacg
    17701 gcgtgccgtt ctctcaaccc gtccgcgccg tcatgtggct gatgcttcac aaacgtatgc
    17761 cgttcgaaat ggtactcacc aaccccggca catcgggcga gaacggcagt cgtcacccgg
    17821 actatctggc caagaatccg ggcggcacca tcccgaccat cgaggagccc gataccggct
    17881 tcaccctggg cgaagcgcac gccatcctct gctatctgtg caacaaatac ggctggtcgg
    17941 atatgtatcc agccgagcct caggagcgcg cccgcgtgga ctggtacctg cactatcacc
    18001 atcgcaacgt cagagaggca tcgaccgggt tggtggcgcc gaagatccgg aaagatctga
    18061 acattccgga gaccgtccag gcggccgccc aggccaccct gacccgggcg ctgcaggcca
    18121 tcgagaccgg ctggctcgga aagtccgcat ttctcgccgg cgatgcggtc accatcgccg
    18181 acttcgccgc ctacgtggag atcggccaac tgcaacccgg cttcaccaac gtctatgatt
    18241 tcaacccgtt tccaaacgtt cgccgctgga tggactcgct caaaaccttc gacagtcacg
    18301 acgacgtcca tgtcgtgctc gccgagctgg gcgacatcag cgccgagccg ccttccatgg
    18361 aggcaatcaa gaacgccaac atctccgctc tgagaacgct caaggcgcgc ctggcggaga
    18421 tgagcggatg acccggcaat cctgaacacg agcaaaggaa taatcatgag cgaaactcac
    18481 aagggcgcct gcttctgcgg agcggtccag atcgaggtca ccggagagcc caacatgatg
    18541 ggttactgcc actgcacgga ttgcgcggcc tgggccgggg ccccggtgaa cgcgttcagc
    18601 ctgtggccgc cggaaaacgt ccaggtgacg gcgggcgagg agaagctggg cacctatgcc
    18661 aaaaccgaag gcagccagcg caagttctgc acgcaatgcg gcggccacgt gttcaccgac
    18721 catcccggga tgggactaat cgacgtttat ctcaacgtcg tgcccggtaa ggcccacacg
    18781 ggcgccatgc acgtcttcta cggcgagaag acgatgtcgg taccggacgg tttgccgaaa
    18841 taccgggatc ttccaaaaga gttcggcggt tccggcgaaa ccatcgccga gtaggaccgc
    18901 cgcgagcggt gagcggagga cccggggtcc ggtctgcacg ggccgccgat tgagacacgg
    18961 tttactcgcg cagcagaacg tgggcgtaga tcggcctgcg gtgttcgaag ccgagcctct
    19021 cgtagattcc gatggcggtg gtctcgctcg ggctgccggc caccacgtgc agaaacgccc
    19081 gatcgccacg ggcaaagcat tcggccagca cgttcagaac cattcgcaag gccagccccc
    19141 gaccgcgcgc gtcgggatgg gtacacacgc cgctgacctc cgtgcagccg ggcagtttca
    19201 tgcgctcgcc agccatggcg accagccggc cgccgctgcg cacgcccaca tagcgcccca
    19261 tctcgatggt ccgctgcaga aacggcccgg gctcggtcag ctcggcaagg gcaaccatct
    19321 ccggagcgtc ggacggcgtc agctcgtcga agggctccgg ctgccccggc ggcgtctcgc
    19381 agaccatctg gtgcagcggc atgacccgca gcagccgcca gcccgcggat tcggggagcc
    19441 ggggctgggt cgaaagaacg ccgacgccgg cgcccgatgg caccagaccg gcaaggtcga
    19501 gcagggcttc aggcgtcgct tcggaaaggc cggcgagcgg cgataccagc gggtcgtaga
    19561 cggcagccag ctcgcttgcc cgaccaagac cagcgtgagc gtgttgcagc gccgcgaaag
    19621 ccggattgtc gagcagatcg tctgacatca gcaccagctc cgattcggtg cggcccctct
    19681 gccacgacgt gcaaagcggc ggctcacttt ctgcattctt gcccatcgag ccgaagatag
    19741 ttcgagccag ggggcgtgtc taacggccct cgcccggcaa taggtgtgcg ctgctagtca
    19801 gtcccccgcc attcccgtcc agcggtaagc cgctggcgtt ttgcgaattt cggctgccgc
    19861 ccggcgagaa aatcttcgaa cacctcggca gtgaactccc cggcgctgtt gaccataccg
    19921 gacgcctcta cctcgtagtc caggcccgag cggtagtccg tttcccagac cttgttcatc
    19981 gcaagcttgg tgtagccgag cgccaccgga ccgcagatct tcaacctttc acagagggcg
    20041 gagacggcct catccacctc gtcgagcgga accgcacggg tgatcaagcc gatctccgcg
    20101 gcttccctgc cggtcagctc cacgccttcc atgaggatct ccttcgcctt gcgccagccc
    20161 accattctcg ggatcctctg ggtaccgccc cagccgcaga tcattccgag cgtcgcctcc
    20221 ggctggctga agcgcgcggc ctcggaggcg atgacgaaat cgcaagcgtg gagattttcg
    20281 aagcctccgc ctacagcccc cgccttggcc acgcccacga cgggtttctc cagcgtctcg
    20341 atcaggcgga aaagatcatg tcccacctgg atgacctcgc gaaactccag gggggagaac
    20401 ttccagtcgc tgcgcgcctc gccgaaaacc acggccccca tgtgaaagcc ctcgccccct
    20461 gcagtgatga ccacgacaca aacgtcagcg tcgttgttgg catcgatgag cgcagcccgc
    20521 atctccgtga gcacggcaag cgtcatgccg tactgcggcg ggttgttctt ggtgatggtg
    20581 gcaacgccgt ccgtcttttc gtagcgaatg gcctcgaacg ccatatggtt ttcctcccag
    20641 gggtttgcgc ctataaacgc ccagaccagc acgaagagcg tgcagccgag ctggtcgacc
    20701 cccagcgcgc gatgcaacgc aaggagagcc ggccctcaat gctcccgaag ctcgtccaga
    20761 tagaagacat tgccgtcggg atcttcgaac aacgccatgt aaccgcccca cggttgtcgc
    20821 tcggggggag ccgtgaactc gacccctgtg ctgcgaagcg tttcgtaggc atcgtccaga
    20881 ctttcaactc cccagccgat cccggtatgt cgccccggag caaaggccgg ttcagcgtcc
    20941 tggccaaccg cggcaatggc gaacccgacg cccttggtat cgaaggacgc gtagccgaac
    21001 tcctcatcgg ccatcgcgac ctcgagaccc agcttgtccc gatagaaggc cagcgccgta
    21061 tcgaaatcac tgacaaagac gttcacgtac cctatggcca gatcaggcat tctgctctcc
    21121 cgaacgccac gacaagacca tacgatacca ccactgcggg gtgcgcgagc gcttacatga
    21181 ccaatgccgg ggcgcgatcg gccacacgct aggccgctgc ggtcaaacga tcaatcgata
    21241 gggggcgctt cgttggtcgt cccttcgccc tcagcaaccg acgcgttgac ctgaccgctg
    21301 aattcgatgt ccggcacggc ggcgccttca gccgcgtccg tggcgggatc gtcatcgagt
    21361 ccctgacagg taaaggcaac cgtgtagtcg ccaggatcca ggtagtgcac cgtgtaggta
    21421 tatgcacccg ccttctccgg gttggaccgg accggcgccg tggtcacggg ctcgccgaag
    21481 ctgccgagat catcgggcgt cgtgtcgtga tttcggtaaa catacaccag gttgccgtcg
    21541 ccttcggcgt tgctcgtgca ggactcagcc atgatcaaag catccgccac gctgccggtc
    21601 aggctgccgt actgggtcat gtcaatcagt cgcagcgccg gggtgagctt gtacgcatcc
    21661 ttggcgtctg gactcgggct caccagcccc tccggatcgg tcagcccttt gcggacgtcc
    21721 cagtcgagaa gcaccgcgtt gctcttgccg gcggtcacca caaaaccgct caccagacgt
    21781 accgtgccgc tcggcacctc cagctcgacc atgccgttgg atttgctctc caccacgtac
    21841 gaatccagaa tcccgtcgat ctccgcgttg gtgtgcagtt cgatccagtt gtagcggccc
    21901 gcgtcgacgg tctgggcatc cagcaacgga aacacgctgc cgttctgcag gtcgaggagc
    21961 tgaaccttct cggagagcgc aaagtcgatg gcaggcccgc tctggggctt gagcgtgatg
    22021 ccacggaatt caacgtgaac cgccatcacg ccgtcgacgg gggcatccgt caggccgacg
    22081 gtcaacgtgc cggtggatgg accctcatcg gatccgctgg atcccccaca tcccaacatt
    22141 gaccaggtca acgccgcaaa gacaatcgaa cggtacgcag acataactcc ttctcctgtt
    22201 gaccttcgac cacgcggctg tttccacgcg tgaacgagac ctaagcaggt atgacgcatc
    22261 gggtcgccga tcggctgcga cgcatgcgtc gatctgccaa atggttcacc cccgaggcgt
    22321 tagcggcttc acgctcgggt ggctgtgacc gagaaagtca tgcggtgctg ataagcggtg
    22381 cagagccgcg caagccaggg tcgaaacgag aaacggccga cttctgctaa cctcgatcca
    22441 atcacaatct ccgggagaac cattgtggcg cgcgcaaagt tgttggagct tttgcaggtg
    22501 gcaggcaatc tggggctgat cgccgggctg attctggtcg gcgttcagat caccgactcg
    22561 aaccgcatcg cctcagcgga gctcttcggt agcagcgtcg gctccgtcat ctcaatcaac
    22621 accgagcagc tcggcgactc acctggtgag gcgatgtcac gcgtgctgta cgaccctgct
    22681 gccgcggaaa cctcggacct ctacatcgcc gaccgaattt acgacgcgct gctgcgccag
    22741 ctggtccgtg tgcacgtatt cgacaccatg gggctttacg gtgacgacga gctcgtcagc
    22801 cccgcggggt tcgtaggcct tcacttcgag ttcttcgcct gtccctatgg cctcgcctgg
    22861 ctcgaccagc aaatcgagct gtttgacacc cgcggcaacg aggagctggc aattctcacg
    22921 agcctgaaga tgctgcggga gctcgcgggc gccgaaagcg cgcgcgtgag gctcgaggat
    22981 cgcctggagc gggtgggggc cctgctggcg cagctggacg acagctgaag tcataacgac
    23041 ggccgctcgc gggcgcacgc cctttggtat tggtatgctc tgcaacagat aaacgaacgt
    23101 ctgcccggta aaggggcaac agcggtccga tagatccgga cgagcacccg ctgcgaggcg
    23161 ccaggagtcc agcccatgat cgacctgaac cgcccggtga atctggcttc gccggagttc
    23221 gcgaaggaca agtacgaatg gtacgagcgc atccgcgagg agcgcccggt gttccagacc
    23281 aagatcagcg tgcttaccgt ctacaccgtt tcccgctacg aggactgcac gaacgtcctc
    23341 aaggacccga acgttctgcg caaccgcacc acggccaccg gaggaagccg cttccccttc
    23401 cccatgccga agtccgtcca gccgctcgtg cagagcatga tccaggaaga cgacccgaat
    23461 caccggcgcc tgcgcgagct ggtcagacgg gccttccggc cccaagccat cgagcagctg
    23521 gagagccaga tcgacgccta ctcccacgaa ctgctcgacg ggctggcgcg cagggacagc
    23581 ttcgatcttc aggccgacta cgcgcgaccg atccccatcc gcatgatcgg cgacatgatg
    23641 gggctttccc gcgaggcggt accgcaattt cagaaggcga tccgagtgct ctcggagggc
    23701 ttcaacgggc tgaaggtgct gcgaacgctg tttctggatc tgcccggcgc cgttcgtttc
    23761 gtccgcgccc tgctcgacga gaaacgctcg atgccgggcg acgacgtcct caccgggctc
    23821 atcgaagccg aggacgatgg cgaccggctc accgacgacg agctggtggc catggtattt
    23881 ctgttgatcg tcggtgggtt cgagacgacg ctgcatctca tcaccaacgg ggtcgtaacg
    23941 ctcctcgagc accccgagca gctgcagcgg ctgaaggaag atcccggcct gatcgattcg
    24001 gcggtggagg agatcctccg acaccggggt cccgtgcacg gcaccaagcc gggctacgcc
    24061 cgggaggaca tcacgcttca tggcatgacg attccgaagg ggaagcccat catgccgctg
    24121 cttgccgccg ccaatcacga cccacgggtg ttcgagaaac cacagacatt cgacatcgcc
    24181 cgctcaccca atcgtcacct cggcttcggc catggcgtgc acttctgcct gggcgcacac
    24241 ctcgcccggg ccgaagcgcg ggtggcgctg cgcaacctat tcgagcgatt tcccgacctg
    24301 cagctggccg tggatccggc gtccctcaag ctgcaggcca tgccgggctg gcaccgctac
    24361 gacgggctgc ccgtgattgc cgggaaaatg cgctcagccg cctagctgcg atcagcgacg
    24421 cgagcctgcc gcgtcctctt cgatcaggtc gcttgcccgc caacccagcg ccatggccgg
    24481 ggcattggtg ttgcccgaga ccagggaggg catcacagag caatcggcga cgcgcaaacc
    24541 ttcgacgccc ctgacccgca accggccgtc caccaccgcg ccttcgtccc ggcccatccg
    24601 gcaggtggcc acaccgtgca ggccgctggt ggaaagccgc cgcacggcgt tgaggatctc
    24661 ctcgtccgtc tggcaggtcg gcccggggat cagctcgtcg ccgacgtacc tggccagcgc
    24721 tggctggcgc acgtagcggc gcatgtaacg gaccatggcg accgccgccg cgcgatcgta
    24781 atccgtgctg agccagttgg gcacgatcac cggcgggtcg tcaggatcgg cggagcgcag
    24841 acgcaccgtg ccttcgcttg tcagattcag gagctggccg tagatggtca tgcccggctt
    24901 gctatcgggc gacgccagct gaactggaaa gttgtcgttg ctgcgcgcgt agttgaaggc
    24961 gccgaggtag agctgcgtgt ccggccggtt ggcgccgggc acggcgcgga cgaaggcccc
    25021 gacctcgaag ggtccggtgg cgagcgggcc gccgcgggtc gccacgtact gcagcgcgct
    25081 gcccaccaga cccaggccgc gcagccgatg gttcaggccc ttggttccct taagcttgtg
    25141 cggcatcgaa aaacccagat gctcgcgcat ccgcgcgccc acgtcgggaa cgtcgcgcac
    25201 cacttcgatg ccgtgatccc gcaggtggtc ggccgggccg atgccggaga gctgcagaat
    25261 cttgggagag aggatggtgc cggccgagag gatgatctcg ccccgggcct caaagacccg
    25321 cctgtcaccc gcgacgcgac acgccaccgc cacggcccga ttgccatcga agcacacccg
    25381 atcgacgtgg gcctgcgact gcacgtgcag gttggggcgc cgccgggcgg ccttcagaaa
    25441 agtgcgcgcc gcgctctgcc gacgtccgcg gcggatgttg tgagcatagt agccaacacc
    25501 ctcgagcatc tcgtcgttga ggtcgtcctt gcgctccagg cccatttcgc ggccggcctc
    25561 gatcatggcc tcggctgcgg gatagcgcag cttgcccgtg ctgacgtgca ccagcccacc
    25621 cgctccgcgg tggtcggctg accccagctc gtgatcctcg atggagcgat aagccgcctt
    25681 catctccgcc cagccccacg ccgggccgcc aagcgccatc cactcctcgt agtcctcggg
    25741 atgcccgcgg gagtagatca tgccgttgat ggagctggaa ccgccgacca ccttgccccg
    25801 cacccacacc tcggaggccg gcaggtccgg ctgccgcggc tgctcgaccg gaaagaacca
    25861 ggcgtggtcg gggctcctga ccagcttccc gatgcccttg ggtatgccga tgagggggct
    25921 cctgtccacc ccacccgcct ccaggagcag aacgttcacg ccgggatctg cggagagccg
    25981 ataggccagc acgcagccgg ccgaaccagc accgacgatg atgtagtcgt acgcctgggc
    26041 catctcagcc ccccgccagc gcttcttcga cgaaatccag gcgatcctgg ccgaagaaca
    26101 tctcgtcgcc cacgaagaag gtcggagcgc cgaaggcacc ccgatcggca gcttcctggg
    26161 tgttctgctt gagcaggttc ttcacctcgt cggtctcgat ggcggcaagg tagcgggatg
    26221 catcgagacc ggcggcagcg atgacctcgc cgagcacctc ggggtccgac aggtcgcggc
    26281 cgtccaccca catggccggg tacgccgtgg ccatgagctg ctccactgcg ccctctttct
    26341 ccgccagcgc agcggcacgg tcggccggca ggatgttgat gatgccatgg ggattgggat
    26401 tcaaggtgac gccgtaacgg cgcgcaaatc gctgcaggtc gccggctatc cagcgtgcct
    26461 tgttcggcac catgaccggc gaacggttct ccgtaagctg gtgcaggccg atggtaaaca
    26521 tgggtcgcca gacggcgctg gcgccggtcc gctggacgat gcccggcacc tgggtccacg
    26581 ccagatagct cggcgggctt aagaagtcga agaaaatttc cacggttttg ctcacgatga
    26641 caccacctcc aaccgtctac cagggctcca gccaggggcg cagctgggtt tcgtgaagcc
    26701 atgcgctgcg gggctggcag tgaatgttcc aatacgtctc ggcgatgtcg tcgggaagca
    26761 gaacgccgtc cgcaggacgc tcctcgaaaa ccgacggcat cacgcggcgg atgagcggcg
    26821 tgtcgatggg cccgtcgatg atcacgtggg ccacgtggat gttctgcggg aaaagctccc
    26881 gggccatgct ctcgctcaat gcgcgaagcg cgtgcttcga ccctgcaaag gccgcgagcc
    26941 gggctttccc gcgcagcgcg gaggtggcgc ctgtgaagag gatggtccca cgcccccgct
    27001 ccaccatgca ccgggacacc tcccgcccca tgacgaaggc accgaaggtg ttgatgcgcc
    27061 agacgcgctc gtaggcatct gccgggaact cggtgatgct gtgggatgca cccacggcgg
    27121 cgttgtagca ggccacctcg atgggtccga cctcggcttc cacccgctcg accagatcca
    27181 cgacctgggc ttcttcggtc gcatcgcagc tgtaaccatg ggcccgtccg cccgccgcgg
    27241 cgatctcgtc caccagcccc tgcagctttt ctccctgacg tcgcgccatg caggcgatga
    27301 aaccctcccg ggcaaaacgc cgcgccaccg cgccgccggt ggcgaccccg gccccgatga
    27361 cgagcaccac cttctccgaa gttgtcatag gcaatccccc ttggcccgac agtaacccac
    27421 ccggattccc gaatatagcg cgagccgccg gtcaggagtt cagacgaaga acatcggttc
    27481 cgtgatctcg agcccgaact ccagccggcc cgcctgctcc agccaggacg gctgggcgag
    27541 gatgtggcgg agcatcgcgt gcatgtcgcg atgggcgcgc tcgagggggt tgtccacgta
    27601 gagggcggcg gtgccgacga gggcaaaggc accgtccaca gtatcccggg cgatcgcgat
    27661 ggcgtgctgg gcggcggcga agagcgcggc gacgtgcgca gggtcgcccg tccggccagc
    27721 caccgcatct tcccagacct gctccgtaga tgcgtaaagg tagcttcggg ccgcctcgcc
    27781 ggccgcgctc tgcgcggccg caaacgactg ggcggcggac cggtcacgca agtcgggcat
    27841 gcgcgacccg gtggtcttgg tgcgcgcgag cgtcacgagc gtctcccgag ccatccccgc
    27901 tatgcccagc atctgggcgg cgaaaccggc cgacatgttg cagaatacgg gcaaccgctc
    27961 caaaggatgg gcgtccccgg gcgggtcggc cagggaaaac gtgaatcttt cggggaccag
    28021 ctgaccggtc accctcacgt gatggctgcc cgtgccgcgc aagcctccgg tatgccaggt
    28081 gtcctcgatc tcgtaggcgc ccctgcgcag gaaagccatg cgcatctcgg gcacctccgg
    28141 ggtgagcatg ctcgtctcgc cgccctcctc gacgatgagc aggaaggcga tccactcggc
    28201 gtgctcgcag cccgagacca gagcccagcg gccgctcacc cggtagccgt gctcctccct
    28261 cacggcccgc cccgatggcc gcgtcgagct ggcgtagagc caacccggat ccgcgaatat
    28321 ctccgcccgg gcggaccgct gtagatagcg ggcgagcagg cagggcagag cgttgttcca
    28381 gaccatccag gagagcgagg cctccgaggc ggacaggatc gccagaacgt cgagaaaggc
    28441 ggggggcgtg acgccggcgc cctcgaggga tccgggcagc agcaggcgac agagccgact
    28501 ggcggccagc gcttcgacca gcggcggggc gaggcggcga tcctgctcgg tctcgccgcg
    28561 acgtgcccat gccagggatg ccaattccgt tgctttgctt gtcagcccgt ccctcatcgg
    28621 ttacgcctca actcgttgac gatgcgctcc aggtgggggc gccgcgccat gcccgtcagc
    28681 tggtcttgat gccgccgcgc acggtgacgt gccagctctt cgggccttcg tcgtccacat
    28741 acccggagat gttgccgacc tcgaaccagt gcagccgggg gatggcgagc cgggccttgg
    28801 acacggcgtc gcgcaccgcc tgctccaggg actcgtccga gcgcccgacg aactgaccga
    28861 tcggcgctcg gggcttcgaa aactccgggt ccgacgtcga cgcctgctcg agtgccacat
    28921 cgcgcatccg ctcccggccg gcttcgagca tcacggcggc ttccgcctca gagagcttca
    28981 gctcgcttct caaccggcgc agaaaagctt cctcttcggc gctgatcttc agatctgaca
    29041 gcacctcgtc cagcgccttc ccgtaaactt cgtgggcaac atcgtcgtga ataagggcta
    29101 cgtccgccgc gcccagataa agcaggtgct gcagatgggc gagatcggcg tgctcggctt
    29161 cggtgagctc cttgtcctcg aggcagaacg ccagatagcg ccggtagaga tgcttgcgct
    29221 cgctcaggaa acggcgggca agctgaacgc ccaggctgct gcagacttcg tccaggctgt
    29281 cgacggcgac gtcctgcagc cgttcgcaac gggccagaag atcctcgatg gcctcaaagg
    29341 tattcacgcg cttggatcgg atgctgcgcg agaggccggg gctggccggc tgagccgggc
    29401 gaaagggtgc tgccacgtcg tgtttccaat cgtttgaggt gattcccacg agggggacat
    29461 ggtaccggcg gcgcttcatg ggatacagtt tcggaaacag cacgaacacg ggagatcgta
    29521 gacatgacga ctggcagacc ggttgccgtg gtcatcggcg ccacttccaa gtggcaggcc
    29581 gacggccgca acacccgtct cgcccacggg cacgacgtgg acgattccga cctgcccgtc
    29641 tcggcgcgtt ggggcatcgg cggcgcggtg gcgcagaagt tcgcgcgtga aggctttctc
    29701 gtggtgctga ccacacgaac ggcggccaac gcggccggcc tggaggcggc catcgccgag
    29761 cagggcggcg agtgcatgac cgtcgagctc gacctggtgt cggagcggtc cgtcagcgaa
    29821 gccttcgcga ccatccgacg ggaggccggc gagcccgagg tggtggtcta caacgcgggc
    29881 tacctcgagg gccgggacct gccgcccgat caggaactcc tggaacacat tcccgtggag
    29941 atgttcgaca ccgcccagca catctccagc cgcggcccct ttctcgtcgc caaggagatc
    30001 ctgccgggca tgcgcgaacg cggctccgga tcgttcctca tcaccaacaa cgcggcctca
    30061 ctgcggggtc gtaagcgcta cacggggcag tcgctgtact acccgagggt gatgatgcgc
    30121 acgctggccc aggtgttgac cgaggagtac tcggaacatg gcgtccacgt ggccaacatc
    30181 atcgtcgacg ggctcatcga ctcgccgggc acacgggctc tgcccatggc gcagcagcgc
    30241 ccggagatca tcatgaaccc ggccgccatc gccgacgcct actactacct gcacacccag
    30301 gacagatcat gctggaccca cgagctgcag ctgacgccgc actcgaccaa gccaagcttc
    30361 tgagcctcgc ctgcaggaca agcggcccgc ccagccaaag cctcagctga cgctggcgcc
    30421 gccgtccacg gggagagcga cccccgtaat gaaccgggcg cggtcggagg caagaaaggc
    30481 tgccgcgtgg gccacgtccc aggccgttcc catcttgcgc cccagcggca cacgccggtc
    30541 acggtcggcg atgacctcct cccgggtcat ggtctgggag acgcggggct ccacggccat
    30601 gggcgtgttc atcaggccag gcaggatcac gttggctcga atgccatagc gggcgttggc
    30661 ctgggcgagc tgctcggtta gggcgatgac accggccttc gtggtcttgt aacccacaaa
    30721 gggatacggc ttccacgcgg ccatggaaga gatgttgatg atcactcctg agccccgctc
    30781 gcgcatgccg ggcagaacgt gcttggcggt gagcaccatg ccgcgcaggt tgatgcgcac
    30841 cagctgatcg aacacgtccg ccgagatctc ttccacgccg gcatcggccc cggcaagccc
    30901 gacgccgacg ttgttgtgga gcacgtcgat gcgtccccaa cggtcgacgg cggcagccat
    30961 gaacgaggcg acgctctctt ccaccgtcac gtcaatttcg gcggacgcgg cctcgccgcc
    31021 ttcatcgagg atcatggcaa cggtttcctc cgccgagccc acgtcgcggt cgcccaccag
    31081 cacccgcgcg ccctcccgag cgaactggat tgccgtggcg cggccgttgc cgatggtctc
    31141 ccccggcgtc tggccaccgc ccacaaccac cgcgatcttg ttttccagca gcccgttggc
    31201 cgtcatgtca gttctccagg tagcggcgcg ggcgaggtcg tcttcagctc agcttgcgaa
    31261 gttccagacg atcagcgctc ccgccagaac gagccagctc tgccaggtcc ggcgcaggat
    31321 gacgcgggcg ttggcgcctt cggccgacgt cctcgcaatg tcgtctcccc gcatcaccct
    31381 gacgatctgc ggcacgtcca cggaaaggga ttgcacgacg aaggcgagcc cgcccacggc
    31441 aaaacccgcg cccgcggggc cgagcccacc atgcagatag acggagaggg caatgctcat
    31501 cagggcaaca taggcgatca ccccgcccca gctcagccag aggaactggc ggcggctgtg
    31561 cgcctggatg tcgaagtggc cgatgagggc gccggcgtag ggcgccagcg gcatggcagc
    31621 gccggtcagc cgggcgccga tcaggtgccc ccactcgtga tagacgtagc agagcacgta
    31681 agccgctacg aacccgaggg ccggagcgac gagcgcagca gccagcgaac cttccgcctc
    31741 gtgccaggcc gccaacccca gcagcatcgc ggcgagccct gaggtgatga ggacgtcgcg
    31801 aattatccag ccggcaagcg tcggccgcgg cgatggcgag agggcttcgg acaccgcgcc
    31861 ggactaacgc caggccaccg gcaggttgcg ccggccccac aggaagaatg gcgctatgcg
    31921 ttgacccggg ccgaggagct tcagatccgg catgcgccgc gccagcgcct tgagcgcgat
    31981 ctccgcttcg agcctcgcca tgggggcgcc gaggcagaag tgcacgccga gcccgaacga
    32041 catgtgccgc ttggcttccc gatgcaggtc gaactcgtcc ggacgctcga agacagaggc
    32101 atcgcggttg gcggcggcgt agttgatgta gaccttgctg cctgtcggta tcacggtgtc
    32161 gtgaagcacg acatcacggg tggtgttgcg atagaggccg agcacgggcg ggtcgaaacg
    32221 cagactctcc tccacggcgg cctcgaccag gacggggtct gccaccactt cctgccagcg
    32281 ctcccggtcc tcgaggagcc gccagaccat gttggtgatg agcgacgtgg tggtttcgtt
    32341 cccgcccacg aggagctggg tgagcatgga ttgcgcatcc gcctccgcga tgaacgagcc
    32401 gtcgtcggcg cgagcgccgg caatcatcgt cagcagatca tccggtaccg cttccccggc
    32461 cacgatggcc gcgtggcgct tgcgatagtg gttgaccatg tactcgaaga aggcggcctg
    32521 atccgcctca aaacgcgtgg gatccgacga tcccatggcc gctacctgga cgtcggacca
    32581 gtgcttgaac cgatcgaggt cttcggtggg cacccccagc atgccggcga tgatgatcac
    32641 cggcaggggg aaggcgaaat catcgtgcag gtcgaattcc ggctccccgg caaggatgtc
    32701 gtcgatgagc ttttcggtga gatctgcgac ctgaatacga agcgcttcca tggccttggg
    32761 cgtgaaggcc tgctggacca gcttgcgcat gtaggtgtgc tgaggcggat cgcagagcat
    32821 gccctgaggt tccgtgaacc gcgggccctg gccgtagtgg ctggaaaacg tctcgatgtc
    32881 ccgcagcgct tcctccacgt cggcatagcg ggaaagcgtg aagaacgggg gatcaaaatc
    32941 ctcgcaatga tggacggggc atcggtcgag aagctcccga tggccattgg ccgggtcggc
    33001 catgacctcg ggcgcgaagg gactccaggt catatcgacc ttcctgaagt gcgggctcgc
    33061 cagtatatcg gcgtttgcct aattggacac cgcatgcgcc agcgaccggc aactcgccgg
    33121 gacttgaatc gcacctcgcc gtgcgcaaag cttgattcgg cgaattcggg ggaggacgcg
    33181 gatggaaacc gccaagagct actgccgcta ttgccacgct tactgcgcca tcgacgtgga
    33241 cgtggaggac gggcggcccg tcgccatacg cggcgacgca aaggatccgg tctacggcgg
    33301 gtacacgtgc atcaaggggc gccagctgcc cgagctctac gacgcccccg accgtctgca
    33361 caccgccttg aagaagatgc ccgacgggcg cttcgaggag attcccacgg cccgggccct
    33421 ggacgagatc gccgcgcgca tcgagcggat catcgagcgc gacgggccga gagccgtggc
    33481 cagctactgc ggaacctacg ccttcatgga gtcggccgca ctggcgctgg cgcgcagctt
    33541 ccaccagggg atcggctcgg agtccttcta cacctcggtg accatcgacc agcccggccg
    33601 gcccatcgcc atgtcccgct tcggcacctg gggcggcggc acccacagct tcgccgatgc
    33661 cgacgtggtg atgagcatcg gcaacaacgc cgtggtttcc tcgttttccg tcttcggggg
    33721 catcccgcct ttcaacccct accggcgcat gaacgacgcc atcaaggacg gcaagaagct
    33781 gatcgtcatc gacccccggc gcaccgaggt ggcccgcaag gcgcacctgt tcctgcaggt
    33841 tcggcccggc gaggacccga cgctgctggc gggcatcatc cgcgtgattc tcgacgagga
    33901 tctgcacgat cagcctttct gcgaccagta cgttgacggc ctggatgcca tcaaggccgc
    33961 cgtcgacccg ttcaccctgg agtacgttgc cagacgcgcg gccgtaccgg ccgagcagat
    34021 cgccgaagcc gcccgcacat tcgccgccgg gcccaagggg gtggcggtca cgggcaccgg
    34081 ccccaacatg gccgagcgcg cgccgctgac cgaacacctg gtgctggcgc tcaacaccat
    34141 ctgcggccgg gtcaaccggg cgggcgagcg gctaccgaac ccgggcatcc tgtccccgct
    34201 gacggccgtg aaagccgagg tcatcccgcc cgcgccggcc tggggacacg ggccccgcaa
    34261 ccgggtgcgc gggctgggcc agatcttcgg cgagatgccg accgcagcgc tgagcgacga
    34321 gattctcgag ccgggcgagg ggcaggtacg cgccctgctc tccatcggcg gcaaccccat
    34381 cgtggcgtgg ccggatcaga tcaaaaccaa gcgggctctg gacgcgctgg acctgctggt
    34441 ctgcgtcgac atccgtctct ccgccacggc ccagctcgcc gactacgtga ttcccggcaa
    34501 gatctgcctg gagcgcgagg acatgcccgt gctcacggac aactggtacg acgtgcccta
    34561 cgggcactac accgaggcgg tctgccagcc caacggcgac ctgcgcgaag agtgggagtt
    34621 ctactgggag ctggcccacc ggctgaattc cgacctgccg ctccccggcg ggtctctgcc
    34681 cacgcaggag aagcccggca agcagacggt gctggagcac atgtttccca accccaaggt
    34741 acccatcgcg gacatccgcg cccaggacgg cggccacgtt ttcgaagggg tccaggtcat
    34801 ggtggaaccc ggccaaggcg acgcccgcct gcagctcaca ccgggtggtg tcgcggatga
    34861 gctggccgag gtgcgcgcag agacgctgca ggagaacggc gcgccgggca aggacggaga
    34921 ctactcacat ctgctgatca gccggcgact gaagcatgtg tacaactcct ccggccagca
    34981 gctgccggag atccgcaaga agggcaccac caaccccgcc tacatgaacc ccgaggacgt
    35041 ggcagcgctg ggaatcggtg acggtgacgt cgtccagatc agcagccgcc acggccagat
    35101 tctcggtgtc gcggcggcag ccccggacgt caagcgcggc gtcatctcca tggcccatgc
    35161 ctggggcggc ctgcccggga acgacggtga ggtacgggaa atcggggcca gcacgaaccg
    35221 gctgctcagc aacgataagg aatacgaccc gatcacgggg atggcgcggc aaagcgcaat
    35281 tcccgtgaac gtgcggccgg tggcgctcta gcgcccggtc gctggcgccg gccgtcaggc
    35341 cttgccgaac gtcaggttca gatattccag gccgagcatg atgcccggct ggtgcatgaa
    35401 gttgttgtcc tcgccgtaag cgaagcggtc gaccttatcg agcagagcgt tccacgaaac
    35461 cgtctgctcc aggcgcgaca ggctcgcccc cgggcacacc cgcgcgccct gggagaaagt
    35521 caggtgtcgg gtgccggccc tgcgatccag cttgaggtcc atcgggcatt cccactcgtc
    35581 cggatcgatg ttgcccgccg cccagcgcag atgcagcagc gaacccttcg gcacgtgcac
    35641 gccctggaac acctcgtccc ggctggtggt gcgggtcgaa aggccctgtg tgggcgcgcg
    35701 caggcgcatg gcgtcctcgg tgaatcccct gaccttgctg cgatcggctt tgatctcgtc
    35761 ccagacgccg tcacgctcga tgagcagctg cacctgctcg gacagcgcgt actgggtcgt
    35821 ttccaggccg ccgatcacca tgttgtagac gacgctgttg atctcgttgt cgttgagctt
    35881 gcggccaacc ggcgagtact ccacctcggt gaggaaggtg atcatgtcgt cggccgggtt
    35941 gcggcgcttt tcctcgatga gcgcgatggt gtaggtcttg aactcctcga gcacctcgaa
    36001 ctgggacttc acttcctcct cgggcaggac gctgcggtgg cccttgccga agacgaaagg
    36061 cttcacggta ccgtcgccgt agtacttcag gagcttcaga tcctccagag gccagcccag
    36121 gatgttcgcc atgaccatct gcggcagcgg ctgcgcgaac tcggaaacga aatccaccgt
    36181 gccacggtcg atccagcgat cgatcagctc gtccgccgtg cgccggatca tctcctcgtt
    36241 gcgcccggct cccggcccca cccagggatc ggtcagctcc tgacggtggg cccgccatgt
    36301 ctccggcgtg ggccgcagca ccagcacgcc ccccatggcc gcgttgaccc ggggagtgtc
    36361 gaagggctcg atatgcttgt cgagcagctg ccgggcgatc agcgtctggg ccgggggaaa
    36421 gcgctcctct tcccggacca ccagggcaat gtcctcgtac ttggagagaa tgaaaccgtc
    36481 cgtcccgggc aggaagccct caccggggat ccggtgcacg ggggcttcgt gatgcaggat
    36541 ctcgtaggcc tcgtaccaat gctccgcggc ccccggcgag aacaggtcca cgtcctccag
    36601 atgcaccggg cagcgcatgg ggctgagctc atgattgggt ttcttcgttt ccaagtaacg
    36661 tcctcctgga agttgtcccc agacccatca ctgcccgggg cgaaggcttg gttgcaaaat
    36721 ttgtagcatg gtacaaatcg ctgggaccgg cgtccaagcc cttatctgtt ggcgccgcat
    36781 catgaatccg ctttgttgag catcccgaga tacgaaggca tagtgagcca gctgatcgga
    36841 aatcagcagg ggggaaggag cactcgaaac cgggcgctga aggcactctc aagcggttct
    36901 gccacgcgct ggtcacgccc gcaccccgaa caaccaaaaa aatcgaggaa gacaacatga
    36961 aaaacagaat tcccttcgcg ttgatggccg cgctag
//
LOCUS       DBNHLM010000115         5753 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1452916, whole genome shotgun sequence.
ACCESSION   DBNHLM010000115 DBNHLM010000000
VERSION     DBNHLM010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1452916"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="AC16QF_05400"
     CDS             <1..1144
                     /locus_tag="AC16QF_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6448022.1"
                     /translation="WDYAYTGGDPNGNLENLAGKRIGIIGTGATAVQCVPHLAETSEH
                     LHVFQRTPSSIDARGNRPTDPGWAAKLAPGWQRDRMHNFNVLVTGGYQEEDLVSDGWT
                     DIIRNLGIISARRAQTESLTLEDMAKNVELADFQKMEQIRARVDELVDDPETAEALKP
                     YYRQFCKRPCFHDEYLQAFNRPNVTLVDTDGRGVERITDKGIVANGVEYELDCIIFAT
                     GFEVGTSYSRRAGYEIVGRNGLTLTDKWADGMKTLHGFQTNGFPNCFFMGVTQGGFTA
                     NFPHLLNEQSIHIAYMIDQARNKNAHVLEASPEAEAGWVETIRQMAVFNQNFLESCTP
                     GYYNNEGNPEVARSLLGSQYGGGPEAFFQILRDWREEGGMEGLDFS"
     gene            1184..2062
                     /locus_tag="AC16QF_05405"
     CDS             1184..2062
                     /locus_tag="AC16QF_05405"
                     /inference="COORDINATES: protein motif:HMM:NF004843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448023.1"
                     /translation="MNDFEGKTAVVTGAASGIGLALAERFAAAQMQVVLADIEEAALD
                     QAVKRLEERQARVIGVVTNTMVESSVAELAERALGEFGNVHVLCNNAGVASLGAGGRG
                     IWEVPAADWDWVMGVNFYGVLFGIQAFVPHMLEHGEPGHIVNTASLAGLFPGGGTYGV
                     SKHAVLALTETLYNDLKARGAALSASVLCPGFVSTRIAKAERNRPAGLEIDQGDVSSE
                     MAAAAEALIAQGKDPAEIAEAVFEAIAQDNLYVLPHPAWDDFVRARVDHVLDRRGPAT
                     MDPAELMRRRAGGEAL"
     gene            complement(2059..2805)
                     /locus_tag="AC16QF_05410"
     CDS             complement(2059..2805)
                     /locus_tag="AC16QF_05410"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6448024.1"
                     /translation="MKLLVTGLSGLIGGAAHPVLAADHDVAALNRREVNGTFTWQADI
                     ADYEAIRPAFDGVDAVIHLAAKPGEHFGWDALRVTNVEGTRNVFQAAVEAGVPRVVFA
                     SSGATVAGWEHDEPYRALVEGRYDDLPDRWPMITVDAPPRPRGVYGSTKVWGEALARH
                     FADTTATSFISVRIGFVNAEDRPANARQRSVWCSQRDVVNALVAALGAPEAVRCGTFF
                     ANSRNRYGYRDLDHGRDLLGFEPLDAAEAG"
     gene            complement(2802..3761)
                     /locus_tag="AC16QF_05415"
     CDS             complement(2802..3761)
                     /locus_tag="AC16QF_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6448025.1"
                     /translation="MDEIVPRHIRDPRIETTFVDANGLRFEVDACGSGRRLALCLHGF
                     PEHSFSWRHQLPMLADLGFRAWAPNLRGYGLTSRPFGVDAYAIENLMDDVAGLIDASG
                     CRETVLIAHDWGALVAWYFAMRKVRPLEKLIICNVPHPGAGRASGRSFAQLRKSWYVF
                     FFQLPRLPENLLGRRGARAVADAFRGVAVDPSRFPDEVVDVYRRNAAQPGALEAMINY
                     YRAALRGGGFRRQSALGFPVIETPTLMVWGEEDAALGIETTRGTDDYVANLTLRYLPR
                     VSHWVQQEQPEVVNAMMRAFLENEPVPYLTWEPKLVQAVKEEA"
     gene            complement(3754..4425)
                     /locus_tag="AC16QF_05420"
     CDS             complement(3754..4425)
                     /locus_tag="AC16QF_05420"
                     /inference="COORDINATES: protein motif:HMM:NF044444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6448026.1"
                     /translation="MNVKSTRVRRNPEAARAEILAAAMAALQDVRFSELTVDSLMKRT
                     GMTRSSFYHYFSGLDEIALGMLEEFAADIRRTVDPWLTGELDEPDHRAATLTHLTSMF
                     EVFERHAPSVAAVAQAAGGSQEVYQEWQRGIVDYFIDLTAEFIRRQRALGRSGAADPD
                     RLARALILMNNAAANDNMTRHNPDGPAVIGAVLGDIWNSAIYGPGGDGTSGCPVELKG
                     GSIRG"
     gene            4550..>5753
                     /locus_tag="AC16QF_05425"
     CDS             4550..>5753
                     /locus_tag="AC16QF_05425"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6448027.1"
                     /translation="MNVLASQLEPWAVADRQPRIAIIGAGMSGIAAVVKLRKAGYTHL
                     TVYEKADRVGGTWRENTYPGLSCDVPSRWYSFSFALSSEWTHRFSYGPEIQAYMEKVA
                     DGFGVTDVVRFDTPVTELTYEAPFWRLTTAAGEQQIYDVVISATGILHHPAMPDIEGL
                     EDFAGASFHSARWDHSVDLTGKRVGVIGTGSTAAQIVGDIAGSVGELHVFQRTPQWMV
                     PMPQKTYSARWKRVLKLFPFLKRLAYQYYFRLMVRTFAMATVGDEKMQARISKITRQN
                     LERNVKDPELRRRLTPDYQAACKRLILCSDFYPAITRDNAHLVTDAIERVTPAGIRTA
                     DGRVHELDVLVLATGFDPSAFILPTKVTGEDGVDLESFWQGAPRALRAVAIPRFPNFW
                     MLEGPTGPV"
BASE COUNT         1065 a         1922 c         1845 g          921 t
ORIGIN      
        1 ctgggactac gcctacaccg gcggcgaccc gaacggcaac ctcgagaacc tggcgggcaa
       61 acgcatcggc atcatcggca ccggagccac cgccgttcag tgcgtgccgc atctggccga
      121 gacctctgaa caccttcacg tgttccagcg aacgccatca tccatcgatg cgcgcggcaa
      181 ccggcctacc gacccgggct gggcggcaaa gctcgcgccg ggctggcagc gggaccgcat
      241 gcacaacttc aacgtgctcg tgaccggcgg ctatcaggag gaagatctgg tcagcgatgg
      301 ctggaccgac atcatccgca acctgggcat catctccgcc cgccgcgctc agaccgagag
      361 cctgacgctc gaggacatgg caaagaacgt ggagctggcc gacttccaga agatggagca
      421 gatccgggcg cgcgtggacg agcttgtcga cgatccggaa acggctgagg ctctcaagcc
      481 ctactaccga cagttctgca agagaccgtg ctttcacgac gagtacctgc aggcattcaa
      541 ccgtccaaac gtcaccctgg tggacacgga cggaaggggc gtggagcgca tcacggacaa
      601 agggatcgtg gcaaacggcg tggagtacga gctcgactgc atcatcttcg cgaccggctt
      661 cgaggtaggc acctcgtaca gccgcagggc gggctacgag atcgtcggcc ggaacggcct
      721 gacgctgact gacaagtggg ccgacggcat gaagacctta cacggttttc agaccaacgg
      781 ttttccgaac tgctttttca tgggcgtcac gcaaggcggt tttaccgcca actttcccca
      841 tcttctgaac gagcagagca tccacatcgc ttacatgatc gatcaggcga gaaacaagaa
      901 tgcccacgtt ctggaagcct cccctgaagc ggaggcaggc tgggtagaga ccattcggca
      961 gatggcggtt ttcaaccaga actttctgga atcctgcacg cccggctact acaacaacga
     1021 gggcaacccg gaggtggccc gatcgctgct cggcagccag tacggcggcg ggccggaggc
     1081 gttttttcag attctcaggg actggcgcga ggaggggggc atggagggtc tcgacttttc
     1141 ctgatgattc tcgcgccggg tgtcgaccat agagggagcg gacatgaacg actttgaagg
     1201 caagacggcg gtggtgaccg gcgccgcgag cggcatcggc ctcgccctgg cggaacggtt
     1261 cgcggccgcg cagatgcagg tggtgctggc ggacatcgag gaggccgcgc tggaccaggc
     1321 ggtcaaacga ctcgaagagc ggcaggcgcg agtgattggt gtggtcacca acaccatggt
     1381 ggagtcgtcc gtggccgagc tggccgaacg cgcgctcggg gagttcggca atgttcacgt
     1441 gctctgcaac aacgccggtg tggcgagcct cggcgccggg ggccggggca tctgggaggt
     1501 gccggccgcc gactgggact gggtcatggg cgtgaacttc tacggggtgc tcttcggaat
     1561 ccaggccttc gtgccgcaca tgctcgagca tggtgagccg gggcacatcg tcaacaccgc
     1621 ctcgctggcc ggcctctttc ccggcggggg cacctacggg gtgagcaagc atgcggtgct
     1681 cgccctgacg gaaaccctgt acaacgatct caaggcacgg ggcgcggcgc tcagcgcctc
     1741 cgtcctctgc cccggattcg tcagcacccg catcgccaag gccgagcgaa accgtcccgc
     1801 ggggctcgag atcgaccaag gcgacgtaag cagcgagatg gcggccgctg ccgaagccct
     1861 gatcgcccag ggaaaggatc ccgccgagat cgcggaagcc gtcttcgagg ccattgccca
     1921 ggacaatctc tacgtcctgc cccatccggc atgggacgac ttcgttcgcg cccgggtcga
     1981 tcacgtgctg gacagacgcg gccccgcaac catggacccg gccgagctca tgcgcagacg
     2041 agcgggcggc gaggccctct agccggcttc cgcggcgtcc agcggttcga acccgagcag
     2101 atcgcggccg tggtcgagat cgcggtatcc atagcgattt cgagaattgg cgaaaaaagt
     2161 cccgcaacgc accgcctccg gcgcgcccag ggccgcgacg agggcgttga ccacgtcccg
     2221 ctggctgcac cagacgctgc gctggcgggc gttcgccggc cggtcctcgg cgttgacgaa
     2281 accgatgcgg accgagatga aggacgtggc ggtggtatcg gcaaagtggc gggcaagcgc
     2341 ctcgccccag accttggtgc tgccgtaaac gccgcggggt cgcgggggcg cgtccaccgt
     2401 gatcatcggc cagcgatcgg gcaggtcgtc atagcgcccc tcgacgagcg cgcggtaagg
     2461 ctcgtcgtgc tcccaaccgg ccaccgtggc cccgctggag gcgaaaacga cccgcggcac
     2521 gccggcctcc acggcggcct gaaagacgtt gcgcgttccc tccacgttgg tgacgcgcaa
     2581 agcgtcccag ccgaagtgct caccgggctt cgccgccagg tgaatgacgg cgtcgacccc
     2641 gtcgaacgct ggccgaatgg cttcgtagtc ggcaatgtcg gcctgccagg tgaacgtgcc
     2701 gttcacctcc cggcggttca gcgccgcgac gtcgtggtcg gctgcaagaa ccgggtgggc
     2761 agcgccaccg atgagcccgc tcaagcccgt gacgagaagc ttcacgcttc ctcctttacg
     2821 gcctgcacga gcttcggctc ccaggtgagg tagggaaccg gctcgttctc gagaaaagct
     2881 cgcatcatgg cattgaccac ctcgggctgc tcctgctgaa cccagtgcga gacccgcggc
     2941 aggtaccgca gggtgaggtt cgcgacgtag tcgtcggtgc cacgcgtggt ctcgatgccg
     3001 agcgcagcgt cctcctcgcc ccagaccatg agggtggggg tctcgatgac gggaaagccg
     3061 agcgcgctct ggcggcgaaa accgccgccg cgcaaggccg cccggtaata gttgatcatg
     3121 gcttccagcg cgccgggctg cgccgcgttg cgccggtaaa cgtccaccac ctcgtccggg
     3181 aagcggctcg gatccacggc gaccccccga aacgcatccg ccacggcccg tgcgcctcgg
     3241 cgacccagaa gattctcggg aaggcgggga agctgaaaga agaacacgta ccaggacttc
     3301 cggagctgag cgaacgatcg ccccgatgct ctgcctgccc cgggatgcgg aacgttacag
     3361 atgatcagct tttccagggg acggaccttg cgcatggcga agtaccaggc caccagggct
     3421 ccccagtcgt gggcgatgag caccgtctcc cggcaaccgg acgcatcgat cagccctgcc
     3481 acgtcgtcca tgaggttctc gatggcgtaa gcgtccaccc cgaagggccg cgacgtcagg
     3541 ccgtaacccc tcaagttcgg cgcccaggcc cgaaagccga gatctgccag catgggcagc
     3601 tgatgacgcc aggagaagct gtgctccgga aagccgtgca gacacagcgc gaggcgccgg
     3661 ccggagccac aggcgtccac ctcgaacctg aggccgttgg catcgacgaa cgtcgtttcg
     3721 atgcggggat cccgtatgtg ccgaggaacg atctcatcca cgaatgctgc ctcccttcaa
     3781 ctcaaccggg cacccgctgg tgccatcgcc tcccgggccg tagatggccg agttccagat
     3841 atcgccgaga acggcgccga tcaccgccgg cccatcggga ttgtggcggg tcatgttgtc
     3901 gtttgccgcg gcgttgttca tcagaatcag cgcccgggcc agtcggtccg gatcggcggc
     3961 cccgcttcga cccagcgccc gctgccggcg aatgaactcc gccgtgagat cgatgaagta
     4021 atcgacgatc ccccgctgcc actcctgata gacctcctgg ctcccgccgg ccgcctgggc
     4081 aacggcggca acgctgggcg cgtgccgttc gaacacctcg aacatcgagg tgagatgggt
     4141 aagcgtcgcg gcgcggtgat cgggctcatc cagttccccc gtgagccagg gatctaccgt
     4201 gcggcgaatg tccgccgcga actcctcgag catacccagc gcaatctcgt ccaggccgga
     4261 aaagtagtgg tagaaagagg aacgcgtcat gcccgtccgc ttcatcaagg aatccacggt
     4321 cagctcggag aagcggacgt cctgcagggc ggccatggcc gccgcgagga tctccgcccg
     4381 cgccgcctcc ggattgcgcc tgacccgcgt gctcttgacg ttcatgacct ccattcccgg
     4441 aatgcttcag tcttccaccc ggacattcta ccccgggcaa tccactattg acacgatgtc
     4501 aatatataac ctattttatt gacgatgtgt caataaaggc gggggcggca tgaacgtact
     4561 ggcatctcag ctggaaccct gggcggtggc cgatcgccag ccccgcatcg ccatcatcgg
     4621 ggccggcatg tccggcatcg ccgccgtcgt caagctccgc aaagccggtt acacccacct
     4681 caccgtttac gagaaggccg accgggtcgg cgggacctgg cgcgagaaca cctacccggg
     4741 gctgtcctgc gacgtgccct cacgctggta ctcgttcagc tttgccctga gctccgagtg
     4801 gactcaccga ttctcctacg gcccggagat ccaggcctac atggagaagg tcgccgacgg
     4861 cttcggcgtg acggacgtgg tccggttcga caccccggtg acggagctca cctacgaagc
     4921 accgttctgg cgcctcacta ccgccgcggg cgagcagcag atctacgacg tcgtgatctc
     4981 ggccacgggc atcctccacc atcccgccat gccggacatc gaaggactgg aggatttcgc
     5041 aggagccagc tttcactcgg cccgctggga tcactcggtg gatctcacgg gcaagcgtgt
     5101 gggcgtgatc ggcaccgggt ccacggcagc ccagatcgtc ggcgacatcg ccggctccgt
     5161 tggcgagctc cacgtattcc agagaacgcc ccagtggatg gtgcccatgc cgcagaagac
     5221 ctacagcgcc cgctggaagc gggtgctcaa gctcttcccg ttcttgaagc gcctggccta
     5281 tcagtactac ttccgcctca tggttcgcac cttcgccatg gccaccgtgg gcgatgagaa
     5341 gatgcaggcc cgcatcagca aaatcacccg gcagaacctc gagcgcaacg taaaggaccc
     5401 ggagctgcgc cgcaggctga cgcccgacta ccaggcggcc tgcaagcgcc tcatcctctg
     5461 ctcggacttc tacccagcca tcacccgcga caacgcccac ctggtcacgg acgccatcga
     5521 gcgcgtcacc cccgccggca tccgaaccgc cgacggtcgc gtccacgagc tcgacgttct
     5581 cgtcctggcc accgggttcg atccgagcgc gttcattctg ccgaccaagg tgacgggtga
     5641 agacggggtg gacctggagt ccttctggca gggcgcccca cgagcactga gagccgtggc
     5701 gattccccgc tttcccaact tctggatgct ggagggccct acgggccccg tgg
//
LOCUS       DBNHLM010000116        20129 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_914136, whole genome shotgun sequence.
ACCESSION   DBNHLM010000116 DBNHLM010000000
VERSION     DBNHLM010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 20129)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20129)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20129
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_914136"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="AC16QF_05430"
     CDS             <1..190
                     /locus_tag="AC16QF_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448028.1"
                     /translation="THAIGAETRVQGHVRRVFDLARKPHDFHTGHVVGWTRPVHETTA
                     FYNLLLDVWNIAYVSELL"
     gene            171..1145
                     /locus_tag="AC16QF_05435"
     CDS             171..1145
                     /locus_tag="AC16QF_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR6448029.1"
                     /translation="MSANCSDWLKAGLLCAALAVLGSCVTRAEQVASQSDMSWFARTV
                     SNSPLADADPDDFYEDASKMVRTDIEFHGVVLSEEGNPLKGARVRTSVFDRLADPFEF
                     PFFAFAAREPVFTGDDGRFSVRERGAGMYVTVEVPGWAPVTAPRKLYVYAEGLNQTLP
                     FPTREAPEEFRFERRPPEEELRPIQTGALRLPEDGVPLEVSLRQVAPYGVEPGTGEAI
                     ITCERTADDAAPDDLFDWWCELTVPGGGFQPFRIDMDQAPEEGYVESSRLEYRAADPR
                     WDDRAERSLIIRFADGMYGFIEVSMRMDGDFYVAFSGHWNPTGSTWLD"
     gene            complement(1195..2022)
                     /locus_tag="AC16QF_05440"
     CDS             complement(1195..2022)
                     /locus_tag="AC16QF_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448030.1"
                     /translation="MSLRKFWVVVSGLLLGSVALAGESPKFGDTVSLRLGGMIHKADA
                     TFSDTRIDRPPVELDLKGLGMDKRYDSFWAGLEWQFAKRWGLTLSYSDFSTDGERSVA
                     EGGNFGDLEWSVGAELTSEMDMKIYIVDVTWDFVKTERSHFGLGLGAHVADFSMEIDL
                     SVEGQIGDDTAIIAGGTETAGITAPLPNISMRGGHRFGDNFYLGGTAGYFSLKVGDID
                     GELITARGSLEWRPHKRFGLGIGYQFVSFDVQEKKETKQERYDADFYGPIFFVSAGF"
     gene            2172..2816
                     /locus_tag="AC16QF_05445"
     CDS             2172..2816
                     /locus_tag="AC16QF_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024978.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HIR6448031.1"
                     /translation="MSAANLARSAFAAAVVAMISCSSPGIESFHDYNPNVNFARYQTW
                     SFISTNPMIVSTAAGAVNPLLEGRIMRAVRTDLAGKGFRYADNPENADFVVSFTVGSR
                     EQIKVDQYPASYQVGYSRYHRYGYGGYGYGMSYGTETRVRQYTEGQLAVDVFDVATRS
                     PAFHGSATKRISDSDRENPEALIAAVVGEALTGFPPGSAAGVAQPQLIPYEDQN"
     gene            complement(2873..5164)
                     /locus_tag="AC16QF_05450"
     CDS             complement(2873..5164)
                     /locus_tag="AC16QF_05450"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6448032.1"
                     /translation="MSDHVRGRLLVLPLLLVLLALWGCSRTEEPAPAEPPEPSGAVSA
                     AEIERNSARNAYFGDLHVHTRLSFDAFIFGTRSTPDSAYAYALGEPIDHPAGFQMQLD
                     RPLDFLGVTDHAMYMGMLEEMSNPETRAGQHPIAVGIREAETPASRLEAFQAMFPYLR
                     KQLDGPDDLLDPAVLRSAWQETVAAAERRNDPGRFTSFVAYEYTAGGAERQNLHRNVV
                     FRGAAPELPFSSMDSGNPEDLWAWMDAQRGQGVEALAIPHNSNGSDGLMFELTRVDGS
                     PMDAAYAEQRMRNEPLVEITQVKGTSDAHPLLSPNDEWADFELMEVKVATVEPSRAAG
                     SYVREAYLNGLAMQAEQGFNPYRFGLIGSSDTHVGAGSFDEANYWSKVGIVDATSVQR
                     GSVPMDEPDADGNLYVGGDVATFQDWGASGLAGVWAEANTREDIYDAFRRKETFATTG
                     PRMQVRFFAGFGLEAAEQAGPDRISALYRDGVPMGGDLVGGDTDTAPHFFLWALSDPA
                     SARLQRLQVIKGWIDAEGRSQEKVFDVACSDGLEVDEATHRCPDNGARVNLADCSISQ
                     NVGAAELESLWKDPEFDAGQMAFYYVRALENPTCRWATWDAVRAGVEPRADLQATIQE
                     RVWSSPIWYVPGGLAGDQPEAEPAAASLVGVRWLWVGSTIGSADVEVAEPHRYNLLLH
                     SDGGAEFRLDCNQGRGGYEMEGEQLRLGPIMTTRMACPEDSKDFEFGQQLDGVTGFSM
                     DGGALELRLKSEGDVMRFRAETD"
     gene            5248..6954
                     /gene="ggt"
                     /locus_tag="AC16QF_05455"
     CDS             5248..6954
                     /gene="ggt"
                     /locus_tag="AC16QF_05455"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione gamma-glutamate
                     hydrolase [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR6448033.1"
                     /translation="MRNIPATSIKLVLCALVLAACTPSAGVIDKGEPATPQAAVAMPA
                     THARAVAAAVLRNGGNAVDAAVAAAFTLAVVQPEAGNIGGGGFMTIFMDGEARFLDYR
                     ETAPAAASRNMFLGPDGKPDADESLVGHRASGVPGTVAGLWAAHQRYGTRTWAELVAP
                     AVDLARNGFTVYPALAQYAREALTDLAGTNFAAHFAALTTTRRFRQPDLADTLQRIAE
                     NGADEFYRGRTARLLAAEMARGGGLITREDLAGYRAIWRDPIDFRWRDYRVLTAPPPS
                     SGGVAIAQLLKMYEARGGDFADLGHNSAAYVHLNAELAKRVFADRAVYLGDPDFVSVP
                     VDSLIDDDYLRARASEANPAQITRPRPVPVIAEHNDTTHFSIVDGAGNAIANTYTINT
                     SFGSGVVVTGAGFLMNNEMDDFAVAPNSPNYFGLVGSEANAIAPGKRMLSSMSPTIAL
                     RDGKVALVVGAMGGSAIITSVYQVMLNLEAFGMEAPEALAATRVHHQFPPDDLITYDP
                     SGALPTEAVAGLEALGYRVEPHSWAFGNVQLIWRNREGVLQAASDERFSGASEVLRTP
                     PP"
     gene            complement(6967..9339)
                     /locus_tag="AC16QF_05460"
     CDS             complement(6967..9339)
                     /locus_tag="AC16QF_05460"
                     /inference="COORDINATES: protein motif:HMM:NF017197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HIR6448034.1"
                     /translation="MQTLPVSVSRKIWIPAVIALLLISIGLAAPLAIKRYMEGWLRDR
                     GVAEARITDVDLNPFTGRVRVEYLDAPGAADESGSFEMLELDLELLGLVTGRVMVSRA
                     ILRDAVLDVRRMPDGRFSVGGLMFPLAQAEQEVESDGEGPAGSPRPRLGLAGLLVENV
                     TLTYDDGMLSDRLYIRSLTIDRMVTWEPERAARLVAELELNGAPVGLEAEVFAFADER
                     RATISIDVDAFDVAAFAPLVADLGIESPGGSLSLSLGGQLRREASGRLFGNVAGTVGV
                     DDIAFGLHPLQIDLGKLSLDVDLAGEIEIQSGDSPPAISGQIGVAASDLLVDHRDWPA
                     RLLGLETMEAPSVRVDGLERASSDTLSVRGLRLLEEEEVVGLSVGSASVEGIELDTSA
                     RALRIGEVSLDDLVARLRRGEQGRLMLLGPAAAEEPSAQPEAAPGADDGAPSFELHVG
                     AVSIGGNSALGLEDLSVEPQVTMELVIDRFRLADLGIGPAAGPMVLDVALTQNGAQLS
                     ATGDLALFADARQVKADLEVVHFDLSQLTPYLPNYRVDRGRLSIQTQLGINGDEVAAE
                     NAVLLEKLKVGVKRSGDPGLVEEGLGMPLDVALDLLRDGDDRIKFDLPISGSIHDPKF
                     GTGDIVRLAMQNALQRAALSVATNALQPLGTIMFVGKMAGKAARPRFDPVAFEPGARS
                     LSELGTSYLDKLAGLLLERPRLHLTFCGVAAVQDVAAAEAVEGDGSASADGAPAVVDE
                     AALLELARARGSAVHDYLIDVRGIPAARLFRCHPEYEAESEEGPRVEIML"
     gene            complement(9344..10552)
                     /locus_tag="AC16QF_05465"
     CDS             complement(9344..10552)
                     /locus_tag="AC16QF_05465"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6448035.1"
                     /translation="MKLEDVDPLDPDIVECPYEFYRALRERGVYRVPQRGFFLVGNYD
                     TAMQVIKDTDTFSSKSGVAVPRSPDGSHPSNPREVRTLLTADPPEHRKYRELVNKAFS
                     AKRVASWEPRIREIADELIEGFAGTGTCELVHDFAVPLPLIVICEAMGLPREMLRTFK
                     RWSDGIASLGGMITPEQRRQIAAEQADFAAYVTEMIEERRSNLGDDFVSDLIRARFDG
                     ARPLNIDELTSLVIQFLVAGNETTTNTIASGMWLLLTHPEQLQAAREDPGLIPNLVEE
                     VLRIESPVQAHFRAAARDTELDGVPIPAGAGVGVIFGCCNRDDDKFADAAEFDVRRDN
                     ASRHLAFSQGIHFCPGAPLARLESAIAFERLLERLGNLRLEGDPNDLAHVPSFTHRGL
                     KKLVVRFDPT"
     gene            complement(10716..11876)
                     /locus_tag="AC16QF_05470"
     CDS             complement(10716..11876)
                     /locus_tag="AC16QF_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448036.1"
                     /translation="MKRIPKWLSGGGLAALLLVLGGQAFGKACADLIAGQHTLVGTVC
                     VGVADDSVQVEYTVIAGWSINSVHFFFDDKPTEGELSVNSCPLGPNFPMTDPKDPYDC
                     GNPKLGHFTYNTSGLNTSTYVFSLPLNGIDYLEHGDLCGSEFNIVAHADVTGPEGQTE
                     GAWAVSWGEMEIDGQMVSFPNYILVDPEDPHERPRGNWGFAGMFDMKVNCEGSFKCET
                     AFAKGDYTFETWPHLDFNSNRWGWLLGPFGKGFVANSESFPLYAGAGQNDISKGALAG
                     VVTVSWDTAYEANQVDFKHHNVAVSYNMNPEWFISSTAENDPTQLLPGSVHIFIGAEG
                     ELLTTAPGQYTYVLDQSEAEVYAYSMSPVTVDAEEFWVVAHAEVCSFVSGEE"
     gene            complement(12119..13426)
                     /locus_tag="AC16QF_05475"
     CDS             complement(12119..13426)
                     /locus_tag="AC16QF_05475"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6448037.1"
                     /translation="MEIRRKQVIERIDPKGQNVGSPADKAPDQDLGHELIPKQRYTSA
                     DFMRREWEGIWSRVWLLGCREEQIAEPGDFICTNIGKESVLLVRQADGGVRAFYNVCM
                     HRGNRLAEEGAGHAETFRCSYHDWQYGLDGRFVDIPDLNTFPQGAPPCAGLREIPCDT
                     WASFVWFSLNEDVEPLKDYMGDLVGHLEPYHFERMAMTRWVTAEWNCNWKASVDAFSE
                     SYHTARTHPQLLWYLDDYNVQIDLYDKHSRYLIPFGLLSPRVDSAPEIPPPLKVMLKN
                     AGIDPASYDGPVGGIRKAVQQHMRESQAALGKDYSELNDDQLTDDYNYLIFPNVTCNT
                     HADNLMLFRNRPHPEDPNRMLFDIWMFELLPEGEERPALPRHDFRPEGSTRSLGLVID
                     QDASNLASVQAGMNSAAYPGLWLSDQELRLRHFHKTISDFVGD"
     gene            complement(13440..14201)
                     /locus_tag="AC16QF_05480"
     CDS             complement(13440..14201)
                     /locus_tag="AC16QF_05480"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448038.1"
                     /translation="MDLGLAGKRAIVPGSTKGILRRVVDQLVDEGCDVAICSRSEDTV
                     ARALRELSGGRGKAIGRACDVMDKEDYEAWIAEMAESMGGADIFIPGVSAGGGSDSER
                     NWWKNFEVDVLSTVRGCEAVLPFMRQSGGGAITFVTTTASVETFGGPQAYNALKGSLL
                     VYAKQLSQAVGRDKIRVNCVSPGPIYFEGGVWAMLEDTMAKWFEKTVRDHPQGRLGAP
                     EEVANCILFLSSPAASWVSGTNLIVDGGFTKRVQF"
     gene            complement(14213..15403)
                     /locus_tag="AC16QF_05485"
     CDS             complement(14213..15403)
                     /locus_tag="AC16QF_05485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6448039.1"
                     /translation="MTNDVYLAAGTRSALGNFGGSLKDVPMTELASHVARACIERSGA
                     NPDSVDHMVFTTTCPTDRDSLFSARVVGVKSGIPEMASALDVSRACASGLQAILSAGQ
                     QIESGQSRLALAGGSEMFSRVPYAVTTARWGIVRGNQQLEDMLEWCYRCPFSLEYMGE
                     TAENLTEEFQYDREAMDEYAVSSQARALAAIESGFLARQIVPVDVPDGRGTRTFEVDE
                     SPREGIDMERLARLKPAFREGGKVTAGNSSGVTDGAAAILVGSRGALESESVEPEARV
                     VDWAVVGVPPRIMGHGPVPAIQLLLERTGLSISDIDYFEVNEAFAVVNLHVERHLGIP
                     RDAHNLYGGGISVGHPPGVTGLRMAINAIQHLRETGGRYAILSMCMGGGQGMAMLIEN
                     AARH"
     gene            15532..16401
                     /locus_tag="AC16QF_05490"
     CDS             15532..16401
                     /locus_tag="AC16QF_05490"
                     /EC_number="1.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR6448040.1"
                     /translation="MTVIGVVKEIWRFPVKSMQGSTIETCTISRTGIPGDRGWAMRDE
                     ERQEIQWGKRHPELMLCSASYRGEPEPGQTTPVDVTFPDGETLGSDDPGIHEKLSELV
                     GRRASLWPVQPPEDEAFYRRYKPDEEQFAREIAEVFAREPGEPMPDLSQFPPEILDYV
                     SVPGTFFDNEELNLVTTASIDHLRARNPAANWDIRRFRPNFYVQTPDDMTGLAENEWV
                     GKTIRVGTAVMRITAETPRCGMTVRPQAEIEFDKSILRTIVRESHQNLGVGAHCIEDG
                     RISVGDALVIVDA"
     gene            17013..19067
                     /locus_tag="AC16QF_05495"
     CDS             17013..19067
                     /locus_tag="AC16QF_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HIR6448041.1"
                     /translation="MAREPLGRTLAMLPLLVAMTATAADRQDLRTNAHAISTLHKQTD
                     QGGSPNYHAAFGLGQQEGLTAVSATTDANNVTHTRYRQTLRGVPVWGEQIIISREASG
                     AVRGARGGLIEGLSAHQVPARPTVAGAAALSATKNRIAAGMSQNPVFERESAELVIYL
                     HDGTPRLSWAVSFFADVPGGGEPTRPTVLLDATTGEVIFEYEGLTHAEGTGPGGNAKV
                     GGYHYGSSEPAMDVAASGTTYTMNNANLKTVNLNHGTSGSTAYSYQGPENVHKEINGA
                     YSPLNDAHFFGGVVYDMYNAWVGTPPLTFQLTMRVHYGNNYENAFWNGSAMTFGDGAS
                     TFYPLVSLDVSAHEVSHGFTEQNSNLVYSGQSGGINEAFSDMSGEAAEFYMRGSNDWL
                     VGADIFKSPSGALRYMYDPTLDGRSIGSANDYVNGMDVHYSSGVFNKAFYLLATTGGW
                     GVQKAFQTFARANQLYWSSGETFRTAAMGVQDAASDLGFSTADVEAAFSAVDVNLGDV
                     CPAEQVAELGNGASSGEFGASAGEWRCFRLSVTAGGTDASFVLAKTAKGRGGDADLYV
                     QHAALPDTVRYDCRSVSSTSSESCAIATPLEGDWYVGVYAYSSYPGVTLTGSFTDPSG
                     SGGGGGDGGGSGSTDADSDGYTSDVDCDDNNPDVHPDHNDTKGRWGRDGVDNDCNGTI
                     DG"
     gene            complement(19115..>20129)
                     /locus_tag="AC16QF_05500"
     CDS             complement(19115..>20129)
                     /locus_tag="AC16QF_05500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6448042.1"
                     /translation="RRIGLLGLVVFSAASVLIALAESIEVILWLRALQAVGGGFATVI
                     CMAMVRDAYEPMEAAKRFPVVMLVMLGAPLAAPAIGAALLSFGWPSIFIFLALYGLAV
                     LVAFLPVPETASMRTGTLELGRILPQYAEVITRRVEGRLVPARYILTQGLLMSGTFVF
                     ITNSSFIYLEYFGIAEEWFVVYFGVNILMMMVFTLITTRLIHRVAPDRLWRAARGLQF
                     LAVVMLALGSWLFDLNIWTLTGLLSVVIGANGMINPSASGLYLSYFDRLSGSAVSLMN
                     VAVFLFGSVLGVVTGVFFDGTLKPIVFTMAVAITVGNLNALGIPPPKNLAPDASGESA
                     SAG"
BASE COUNT         3702 a         6748 c         6323 g         3356 t
ORIGIN      
        1 aacccacgcg atcggtgcgg aaacccgcgt tcaggggcac gtccggcggg tgttcgacct
       61 ggcccgaaag ccgcacgact tccatacagg ccacgtggtg ggctggacgc gaccggtcca
      121 cgagaccacg gcgttctaca acctcctcct cgacgtctgg aacatcgcct atgtcagcga
      181 actgctctga ttggctgaaa gccggactcc tctgcgcagc cctcgcggtg ctgggcagct
      241 gcgtgacccg ggccgagcag gtggcgtccc agagcgacat gtcctggttc gcaagaacgg
      301 tgagcaacag cccccttgcc gacgcagacc cggacgattt ctacgaggac gcgtccaaga
      361 tggtccgaac ggatatcgag tttcatggcg tggtgctcag cgaggagggc aacccgctca
      421 agggagcgcg cgtgcggact tccgtcttcg accgcctggc cgacccgttc gagtttccct
      481 tcttcgcgtt cgccgcccga gagccagtct tcacgggcga cgacggccgt ttcagcgttc
      541 gcgaacgggg ggcgggaatg tacgtgaccg tcgaggtccc cggctgggcg cccgtgaccg
      601 cgccccgcaa gctctacgtc tacgcggaag gattgaacca gacgttgccg tttcccaccc
      661 gggaggcgcc ggaggagttc cggttcgagc ggcggcctcc ggaggaagag ctcaggccca
      721 tccagaccgg cgcgttgcgg ctacccgaag acggcgtgcc cctggaggtt tcgctgcgcc
      781 aggtagcacc ctacggcgtg gagccgggca ccggtgaagc catcatcacc tgcgagcgaa
      841 ccgcggacga cgccgcccct gacgatctct tcgactggtg gtgcgagctg accgtgccgg
      901 gcggcggatt tcagcctttc cggatcgaca tggatcaggc gcccgaggag ggttatgtgg
      961 aatccagtcg tctcgagtac cgcgccgccg atccccgctg ggacgaccgc gcagaacgct
     1021 ctctcatcat ccgtttcgca gacgggatgt acggtttcat cgaagtatcc atgcgcatgg
     1081 acggagattt ctacgtagcg ttcagcgggc actggaaccc gaccggctcc acctggctgg
     1141 attagacgtt tcgctgccgc cgcgcggcgt ccgtagctgc ctgagaggcg actcttagaa
     1201 cccggccgag acgaagaaga tggggccgta gaagtcggcg tcgtatctct cttgcttggt
     1261 ctctttcttt tcctgcacgt cgaagctgac gaactggtag ccgatcccga gaccgaaccg
     1321 cttgtgcggc cgccattcaa gagacccccg tgccgtgatc agctcgccgt cgatgtcccc
     1381 gaccttgagg gagaagtatc cggcggtacc ccccaggtag aagttgtcac caaagcggtg
     1441 gccgcctcgc atggagatat tgggcagagg cgccgttatg cccgcggttt cggtcccgcc
     1501 agcaatgatg gctgtatcgt cgccaatctg accctcgacg ctcagatcga tttccatgct
     1561 gaaatcggca acgtgcgcac ccagaccgag accgaaatgg ctgcgttcgg tcttcacgaa
     1621 atcccaggtg acgtcgacga tgtagatctt catgtccatc tcggacgtca actcggcacc
     1681 caccgaccac tccagatcgc cgaagttgcc accctcggcc acgctgcgtt ccccgtcggt
     1741 gctgaaatcg gagtagctca gcgtcaggcc ccatcgcttg gcaaactgcc attcgaggcc
     1801 ggcccagaac gaatcgtagc gtttgtccat gcccagcccc ttcaggtcga gctccacggg
     1861 cgggcggtct atccgggtat ccgagaaggt cgcatcggcc ttgtggatca tgccgcccag
     1921 ccgcagcgag accgtgtctc cgaacttggg gctctcaccc gccagggcga cagaacccaa
     1981 cagaaggcca gacacgacca cccagaattt ccgcaagctc atcgacatct cctacgtgct
     2041 ttgcaacgtc ccagcacttc tgcaaccggc attatacata gccgtgcagc tgcggatcgc
     2101 ggacgtgtgc cgacctccgt ttgccgctat gatcgccccc gcgtcacaaa gcacacacaa
     2161 agggagcacg catgtctgcc gccaaccttg cccgatccgc tttcgcagcc gccgttgttg
     2221 caatgatttc ctgcagcagc ccggggatcg agtcattcca cgactacaac ccgaacgtca
     2281 actttgcgcg gtatcagacg tggtcgttca tttccaccaa tcccatgatc gtctccaccg
     2341 ccgcgggcgc cgtcaatccg ttgctggaag gccggatcat gcgcgcggtg cggaccgacc
     2401 tggcagggaa aggattccgc tatgccgaca atccggagaa cgcggacttc gtcgtttcat
     2461 tcaccgtcgg atcgcgggag cagatcaagg tggaccagta tccggccagc taccaggtcg
     2521 gctacagccg ctatcatcgc tacggttacg gcggctacgg gtacggcatg agctacggca
     2581 cggaaacccg cgtccggcag tacacggaag gacaactcgc cgtggacgtc ttcgacgtag
     2641 ccacacgcag cccggccttt cacggctcgg ccaccaagcg gatatccgac agcgaccggg
     2701 aaaatccgga agcgctgatc gccgccgtcg tgggcgaggc gctcacgggc ttcccgcccg
     2761 ggtcagccgc cggcgtggcg cagcctcagt tgataccgta tgaggatcag aactgatctg
     2821 acaaactcga ctgacgcgcg ggctccggtg atcggagctc aggcgcgctc gatcagtcgg
     2881 tttcggcgcg aaagcgcatc acgtcgcctt cggatttcag gcgcagctcc agggcgccgc
     2941 catccatcga gaacccagtg accccgtcga gctgctggcc gaactcgaag tccttggagt
     3001 cctcggggca ggccatcctt gtcgtcatga tcgggccgag ccggagctgc tcgccttcca
     3061 tctcgtagcc gccacgaccc tgattgcagt cgagacggaa ctccgcgcca ccatctgagt
     3121 gcagcagcag gttatagcga tggggttccg ccacctcgac atccgccgag ccgatcgtcg
     3181 accccaccca gagccaacgc accccgacga ggcttgccgc cgccggctcg gcttccggtt
     3241 gatcgcctgc caagccgccg gggacgtacc agatcggcga tgaccagacg cgctcctgaa
     3301 tcgtagcctg caagtccgct ctgggttcca ccccggcccg gaccgcgtcc caggtagccc
     3361 agcgacaggt ggggttctcc agcgcgcgca cgtagtagaa ggccatctgg ccggcatcga
     3421 actcgggatc cttccagaga ctctccagct cggcggcgcc cacgttctgg ctgatggagc
     3481 agtcggcgag attcacgcgg gcgccgttgt ccgggcatct gtgggtcgcc tcgtcgacct
     3541 cgaggccgtc cgagcacgcc acgtcgaaca ccttttcctg gcttcttccc tctgcgtcga
     3601 tccatccctt gatgacctga agacgctgca agcgcgccga cgccgggtca ctcagagccc
     3661 agaggaaaaa gtgcggcgcg gtatcggtat cgccgccgac tagatcgccg cccatgggca
     3721 cgccgtcgcg ataaagcgcg ctgatccggt cgggccctgc ctgctccgct gcctccagcc
     3781 cgaagcccgc gaagaaccgg acctgcatgc gcgggccggt ggtggcgaag gtctccttac
     3841 gacggaacgc gtcgtatatg tcttcacgcg tgttcgcctc cgcccatacc ccggcgagcc
     3901 cggacgcgcc ccaatcctga aacgttgcaa catctccgcc cacgtagagg ttgccgtcgg
     3961 cgtcgggctc gtccatgggc acggagccgc gctggacgga ggtcgcatcc acgatgccga
     4021 ccttgctcca atagttggcc tcgtcgaagg agcccgcgcc aacgtgggtg tccgacgagc
     4081 cgatgaggcc gaaccggtac gggttgaatc cctgctctgc ctgcatggcc aggccgttga
     4141 ggtaagcctc tcgaacatag ctgccggccg ccctgctggg ctccacggtc gccaccttga
     4201 cctccatcag ctcgaagtcg gcccactcgt cgttgggcga aagcagggga tgggcgtcag
     4261 acgtgccctt gacctgcgtg atctccacca ggggctcgtt gcgcatccgc tgctcggcgt
     4321 aagccgcgtc catgggcgag ccgtccacac gggtcagctc gaacatcagc ccgtcggagc
     4381 cgttggagtt gtgaggaatc gccagcgctt ccacgccctg gccgcgctgg gcgtccatcc
     4441 acgcccacag gtcctccggg ttgccggagt ccatgctgga gaacggcagc tcgggggcgg
     4501 cgccccgaaa aaccacgttg cggtgaaggt tctgccgctc ggctccccct gcggtgtatt
     4561 cgtacgccac gaagctggta aaccggcccg gatcgttgcg gcgctcggca gcagccacgg
     4621 tttcctgcca cgcagagcga agcaccgcgg gatcgagcag atcgtccgga ccatcgagct
     4681 gcttgcgcag ataaggaaac atggcctgga acgcctctag acgcgatgca ggagtctccg
     4741 cctcgcggat gccaaccgct atcgggtgct ggccggcccg ggtttcgggg ttgctcatct
     4801 cctccaacat gcccatgtac atggcgtgat ccgtcactcc cagaaaatcc aggggccggt
     4861 ccagctgcat ctggaaaccg gcgggatgat cgatgggctc gcccagagca taggcgtaag
     4921 ccgagtccgg cgtggagcgc gtgccgaaga tgaaagcgtc gaaagacagc ctcgtgtgca
     4981 cgtgcaggtc gccgaagtag gcgttgcggg cggagtttcg ctcgatctcg gccgccgaca
     5041 cagcaccgct cggctcgggc ggctcggcag gagcgggttc ttcggttcga gagcaccccc
     5101 acagcgccag caggacgagc agtaggggca aaaccagcag ccttcccctg acgtgatcgc
     5161 tcatcatcct gacccctccg aagatctcca cgtttcagat aatgtagcag gctcttagac
     5221 cactgtcggc agcgagcgcc gggggacatg cgcaacatcc cagccaccag tatcaagctt
     5281 gtcctgtgcg ctctcgtgct cgcggcttgc acaccctctg cgggcgtcat tgacaaaggc
     5341 gagcccgcga cgccccaggc ggccgttgcc atgcccgcca ctcacgcccg ggcggtcgcc
     5401 gccgccgtgc tccggaacgg aggcaacgcc gtggatgctg ccgtcgcggc cgccttcacc
     5461 ctcgccgtgg tgcaacccga ggcaggcaac atcggcggcg gcgggttcat gacgatcttc
     5521 atggatgggg aagccaggtt cctggactac cgggagaccg cccccgctgc ggcgagccgc
     5581 aacatgttcc tgggcccgga cggaaagccg gatgccgatg agagcctggt gggccatcgg
     5641 gcctccggcg tgccaggaac cgtggccggc ctgtgggccg ctcaccagcg ctacgggacc
     5701 cgaacgtggg ctgagctggt cgcacccgcc gtggacctgg ctcggaacgg ctttacggtc
     5761 tatcccgccc tcgcccaata cgctcgggag gcacttacgg acctcgccgg cacgaacttc
     5821 gctgcccact tcgccgcgct gacgaccacc aggcgttttc ggcagccgga cctggccgac
     5881 accctccaac gcatcgcaga gaacggggca gacgagttct accgcggccg cacggccaga
     5941 ctcttggccg cggagatggc acgcggcggc gggctgatca ccagagaaga cctggcgggc
     6001 taccgcgcca tatggcgcga tcccatcgac ttccgctggc gcgactaccg ggtgctcacg
     6061 gctccgcccc cgagttcggg cggcgtggcc atcgcccagc tgctgaagat gtacgaggcc
     6121 cgcggcggcg atttcgcgga tctcgggcac aactccgccg cctatgtgca tctcaatgcg
     6181 gaactggcga agcgcgtctt cgccgaccgg gccgtttatc tgggggaccc cgacttcgtc
     6241 tccgtgccgg tcgattcgct gatcgacgat gactaccttc gcgcccgggc cagcgaagcc
     6301 aaccccgcgc agatcactcg tccgcgtccg gttcccgtca tcgccgagca caacgacacc
     6361 acccactttt ccatcgtcga cggggccggc aacgccatcg ccaacacgta caccatcaac
     6421 acgtctttcg gcagcggcgt ggtcgtcacg ggggccgggt ttctgatgaa caacgagatg
     6481 gacgacttcg ccgtggcgcc gaactcgccc aactacttcg gcctggtggg cagcgaggcc
     6541 aacgccatcg cgcccggcaa gcgcatgctt tcctcgatgt cgcccaccat cgcgctgcgc
     6601 gacggcaagg tcgcgctggt cgtcggcgcc atgggcggct cggccatcat cacctcggtc
     6661 tatcaggtga tgctcaacct ggaagccttc gggatggagg cgccggaagc gttggcagca
     6721 acccgcgtgc accaccagtt ccctcccgac gatctgataa cgtacgaccc gtcgggcgcc
     6781 ctgccgaccg aagccgtcgc gggccttgag gcgctcggct atcgggttga gccccactcc
     6841 tgggcgttcg gcaacgttca gctcatctgg cgcaaccgcg agggcgtgct gcaggcggca
     6901 tcggacgagc gcttctcggg cgcgagcgag gtgctgcgaa ctccaccgcc ttgacgctca
     6961 cgcagctcag agcatgattt ccacccgcgg cccttcctcg gattcggcct cgtattccgg
     7021 gtgacagcga aacaggcgcg cggccggtat gccgcgcacg tcgatgaggt agtcgtgcac
     7081 ggccgagccc cgggccctgg caagctcgag cagggctgcc tcatcaacga cggccggggc
     7141 accatccgcg ctcgcgctgc cgtccccctc caccgcttcc gccgctgcca catcctggac
     7201 agcggcgacg ccacagaacg tcaggtgcag acggggccgt tccagcagca ggccggcgag
     7261 cttgtccaga tagctcgtgc ccagctcgct gagcgaccgc gcgccgggct cgaaagccac
     7321 cgggtcgaaa cggggccgtg ccgcctttcc cgccatcttg ccaacgaaca tgatagtgcc
     7381 cagcggctgc agcgcgttcg ttgcgacgct gagcgccgcg cgctgcagcg cgttctgcat
     7441 ggccagccgc acgatgtcgc cagtgccgaa tttcggatcg tggatgctgc cggagatggg
     7501 cagatcgaac ttgatccgat cgtccccgtc ccgaagcaga tccagagcaa cgtccagcgg
     7561 catgccgaga ccctcttcca cgagccccgg atcgcccgac cgtttgacgc ccaccttcag
     7621 cttctccaga agcacggcgt tctcggctgc gacctcgtcg ccgttgattc ccagctgggt
     7681 ctggatcgaa aggcggcccc ggtccacccg gtagtttggc aggtaggggg tcaactggct
     7741 gagatcgaaa tggacgactt ccaggtctgc cttcacctgc cgggcgtcag cgaacaacgc
     7801 cagatccccg gtagcggaga gctgcgcgcc attctgggtg agcgcgacgt ccaggaccat
     7861 gggcccggca gccgggccga tacccagatc ggcaagccga aatctgtcga tgacgagctc
     7921 catggtcacc tgcggctcga cggataagtc ctccaaaccc agggcgctgt ttccgccgat
     7981 gctcactgcc cccacatgaa gctcgaacga cggcgcgccg tcatcggcac ccggcgccgc
     8041 ttccggctga gcagacggct cctcggcggc agccggccca agcagcatga gccgaccttg
     8101 ctctccacgc cgtaaccggg ccacgaggtc gtccagggag acctccccga ttctcaacgc
     8161 gcgagcgctg gtatcgagtt cgatgccctc cacggaggcc gagcccaccg acagcccgac
     8221 gacctcttcc tcttccaaaa gcctgaggcc gcggaccgag agggtgtcag acgacgcgcg
     8281 ctcgagcccg tcgacgcgca ccgacggcgc ctccatggtc tccaaaccca gcaaccgggc
     8341 aggccagtcc ctatggtcta cgaggaggtc gctcgcggcc acgccgatct gcccggagat
     8401 cgcagggggc gagtcacccg attgaatttc gatctccccg gccagatcga cgtcgagact
     8461 cagcttaccc agatcgatct gcaggggatg caaaccgaag gctatgtcat caacccccac
     8521 cgtcccggcc acgttgccga acagccgccc ggaagcctct ctgcgcagct gcccgcccaa
     8581 agaaagcgat agcgatccgc ccggcgactc gatacctaga tcggccacca gcggagcaaa
     8641 cgcggcaaca tcgaaagcat ccacgtcgat cgatatggtg gcgcgccgct cgtctgcgaa
     8701 cgcgaacacc tccgcctcca gcccaaccgg cgccccgttc agctcgagct cggcaaccag
     8761 ccgagcggcc ctctcgggtt cccaggtgac catgcgatcg atggtcagag aacggatgta
     8821 gagccgatcg ctgagcatcc cgtcgtcgta ggtaagggtc acgttctcca ccaggagtcc
     8881 ggctaagccg agcctgggac gcggtgatcc ggcgggaccc tcgccgtcgc tctccacttc
     8941 ctgttccgcc tgggccaggg ggaacatcag gccacccacg gagaagcgcc cgtccggcat
     9001 ccgccgcacg tccaggacgg catcccgcag gatggcccgc gaaaccatca cccgcccggt
     9061 aacgaggccg agcagctcca gatccagctc cagcatctcg aagctgcccg actcatcggc
     9121 ggcgccggga gcatccagat actcgacccg taccctgccg gtgaaggggt tcaagtccac
     9181 gtcggtgata cgggcctcgg caacgccgcg atcacgcagc cagccttcca tgtagcgctt
     9241 gatggccagg ggcgcggcca gcccgatgct gatcagcagc agggcaataa cggccggaat
     9301 ccagatcttt cggctcacgc tgacgggcaa cgtctgcatc tcatcatgtc ggatcgaagc
     9361 gaactaccag cttcttcagg ccacgatggg tgaagctggg gacgtgggca agatcattcg
     9421 gatccccttc gagacgcagg ttgccgagcc gttcgaggag gcgctcgaag gcaatcgcgc
     9481 tttccagacg ggccagcggc gcgcccgggc agaagtgaat gccctggctg aaggcgaggt
     9541 gacgcgaggc gttatcgcgc cggacgtcga attccgccgc gtcggcaaac ttgtcatcgt
     9601 cccgattgca gcagccgaag atgacgccca ccccggctcc cgcggggatc ggcacgccgt
     9661 cgagctccgt gtcccgggcg gcagctctga agtgcgcctg cacgggcgac tcgattctca
     9721 gcacttcctc gacgaggttg gggatcaggc cggggtcctc gcgcgcggcc tggagctgct
     9781 caggatgagt gagcaggagc cacatcccgg acgcgatggt gttggtggtg gtctcgttac
     9841 ctgccaccag aaactggatg acgaggctcg taagctcatc aatgttcagc ggccgtgcgc
     9901 cgtcgaagcg ggctcggatc agatcggaga cgaagtcgtc gccgagattc gatctgcgct
     9961 cctcgatcat ctcggtgacg tatgcggcga aatccgcctg ctccgcggcg atctgccggc
    10021 gttgctccgg cgtgatcatg cccccgaggc tcgcgatccc gtccgaccag cgcttgaagg
    10081 tgcgaagcat ttcccggggc aggcccatgg cctcgcagat gacgatgagc ggcaggggca
    10141 cggcaaagtc gtggacgagc tcgcaggtgc cggtgccggc aaagccttcg atcagctcgt
    10201 cggcgatctc ccggatgcgc ggttcccagg aggccacccg cttggcggag aaggccttgt
    10261 tgaccagctc gcggtacttg cggtgctcgg gggggtccgc cgtgagcagc gtgcgcacct
    10321 cgcgcgggtt cgacgggtgg ctgccgtccg gggatctcgg caccgccacg ccggacttcg
    10381 acgaaaaggt gtccgtatcc ttgatcacct gcatggcggt gtcgtagttg ccgacaagga
    10441 agaatccgcg ctgcggcacc cggtagacgc cgcgctcccg cagcgcccgg taaaactcgt
    10501 acgggcactc gacgatgtcg ggatccagcg gatcgacgtc ttcaagcttc atctgcggtt
    10561 cactcccacc ccccgatgcg attctgcgcg gagcgctgcc ggcgcgcaat cggcccggac
    10621 gtcagcctcg ccccacaagt aaccgagcgg ccaaaaaaag agggtgccga agcaccctcc
    10681 aatccgcctg ttcttctgaa cagtgtcctg tcgatctact cctcgccgga gacgaacgag
    10741 caaacctcag cgtgcgccac cacccagaat tcctcggcat ccaccgtcac cgggctcatg
    10801 ctgtaagcat aaacctcggc ctcgctctga tcgaggacgt aggtgtactg gccaggggcc
    10861 gtcgtgagga gttccccctc agcaccgatg aatatgtgca cggatccggg gagcagctgg
    10921 gtcgggtcgt tctccgcggt gctcgagatg aaccattccg gattcatgtt gtagctgacc
    10981 gcgacgttgt gatgcttgaa gtccacctgg ttggcctcgt aggcggtatc ccagctcacg
    11041 gtgaccacac cagccagagc gcctttggat atgtcgttct gaccggctcc cgcgtagagc
    11101 gggaaggact ccgagttggc gacgaaccct ttgccgaacg gcccgagcag ccagccccaa
    11161 cgattgctgt tgaagtcaag gtggggccag gtctcaaaag tgtaatcccc tttggcgaag
    11221 gctgtctcgc acttaaagct accttcgcag ttgaccttca tgtcgaacat accggcaaag
    11281 ccccagttcc cgcgcgggcg ctcatggggg tcttcaggat ccacgaggat gtagttgggg
    11341 aagctcacca tctgcccatc gatctccatc tctccccagc tgacggccca agcgccttcg
    11401 gtttggcctt cggggcccgt gacatcggcg tgggcaacga tgttgaactc ggacccacag
    11461 agatcaccat gctcgagata gtcgatgccg ttcaacggca ggctgaagac gtaggtcgag
    11521 gtgttcaggc cactcgtgtt gtaggtgaag tgcccgagct tcggattgcc gcagtcgtaa
    11581 ggatccttgg gatcggtcat gggaaaattc ggaccgagcg gacacgaatt caccgaaagc
    11641 tctccttccg tcggcttgtc gtcgaagaag aagtgaacag agttgatcga ccagccggcg
    11701 atcaccgtgt actcgacctg cacgctgtca tcggcaacgc cgacgcaaac ggtaccgacg
    11761 agggtatgct ggcctgcaat caggtcggca caggccttgc cgaaggcctg accgcctaac
    11821 acgagaagca aagcggcgag gccgcctccg ctgagccact tcgggattct cttcacgaga
    11881 tgcacctcct agttgtagag aacgcgcatc cccctcggcg cgaccggctc ctgtttctat
    11941 gtcggattac cgatctgaag attctgacga ttggtgctgg cgttgtggta aaggagtgtg
    12001 tccgctttgt ccgggtgggc cccggttacg tagggcaaat gcaaaagtgt cgaccagttc
    12061 acagaactgg tcggacgacc gtatccaagc tgaaaggccc gccgtcgcgc ggtgctcgtc
    12121 agtcgccgac gaagtcgctg atggtcttgt gaaagtggcg cagccggagc tcctggtcgg
    12181 agagccagag cccgggatac gcggcgctgt tcatgccggc ctggacggac gcgaggttcg
    12241 aggcgtcctg atcgatgacg agcccgagag accgcgtgct gccttccggc cggaagtcgt
    12301 gcctgggcag cgcaggccgc tcttcgccct cgggcagcag ctcgaacatc cagatgtcga
    12361 atagcatccg gttcggatcc tccgggtggg gccggttgcg gaacagcatc agattgtcgg
    12421 cgtgggtatt gcaggtgacg ttggggaaga tcaggtagtt gtaatcgtcg gtcagctgat
    12481 cgtcgttcag ctccgagtag tcctttccaa gggccgcctg gctctcccgc atgtgctgct
    12541 ggacggcctt gcggatcccg cccacgggac cgtcgtagga agcgggatcg atcccggcgt
    12601 tcttgagcat taccttcagc gggggcggta tctccggcgc ggagtccacc cgcgggctca
    12661 gcaggccgaa cgggatcagg taccggctgt gcttgtcgta gaggtcgatc tgcacgttgt
    12721 aatcgtccag ataccaaagc agctgggggt gggtgcgcgc cgtgtggtag ctctcggaga
    12781 aggcgtcgac ggacgccttc cagttgcagt tccactccgc ggtcacccag cgggtcatgg
    12841 ccatgcgctc gaagtggtag ggctcgaggt gccccaccag gtcgcccatg tagtccttca
    12901 gcggctcgac gtcctcgttc aggctgaacc agacgaagct cgcccaggtg tcgcagggga
    12961 tctcgcgaag cccggcacag ggcggggcgc cctgggggaa cgtgttcaga tccggaatgt
    13021 ccacgaagcg gccgtccagg ccgtactgcc agtcgtgata gctgcagcgg aacgtctcgg
    13081 catggccggc tccctcctcg gccagccgat taccgcgatg catgcagacg ttgtagaagg
    13141 cccggacgcc gccatcggcc tggcggacca gaaggaccga ttccttgccg atgttcgtgc
    13201 agatgaagtc gccgggctcg gcgatctgct cctcccggca gcccagcagc cagaccctgc
    13261 tccagatgcc ctcccactcc cgccgcatga aatcggcgga cgtgtagcgc tgcttgggaa
    13321 tcagctcgtg gccgaggtcc tgatccgggg ctttatctgc gggcgagccg acgttctgtc
    13381 ctttcgggtc gatgcgttcg atgacctgtt tgcggcgaat ctccatcagc agggacgctc
    13441 tagaactgca cccgtttggt gaagccgccg tcgacgatca ggttcgtgcc ggacacccag
    13501 ctcgccgccg ggctggacag aaacaggatg cagttggcta cctcctcggg cgcaccgaga
    13561 cgaccctggg ggtgatcgcg cacggtcttc tcgaaccact tggccatggt gtcctctagc
    13621 atggcccaga ccccgccctc gaaatagatc gggccggggg agacgcagtt gacgcgtatc
    13681 ttgtcgcggc ccactgcctg tgagagctgc ttggcgtaga cgagcaggga gcctttcagc
    13741 gcgttgtagg cctgagggcc gccaaatgtt tctactgaag ccgtggtggt aacgaacgtt
    13801 atcgctccgc cgccggactg ccgcatgaag ggcagcaccg cctcgcagcc gcgaacggta
    13861 gagaggacgt ccacctcgaa gttcttccac cagttgcgct cgctgtcgct gccgccaccg
    13921 gcgctgacgc caggaatgaa aatgtccgca ccacccatgg attccgccat ctcggcgatc
    13981 caggcctcgt agtcctcctt gtccatcacg tcgcaggccc ggccgatggc cttgcccctg
    14041 cccccggaca gctcccgcaa ggcacgcgcc acggtatctt cggagcgcga gcagatggcc
    14101 acatcgcagc cctcgtccac gagctggtcc acgacccgcc gcaggatgcc cttggtcgat
    14161 ccgggcacga tggcccgctt gcctgccaat cccaggtcca tcttcgtcgc tcctaatgcc
    14221 gggccgcgtt ctcgatgagc atggccatgc cctggccacc tcccatgcac atcgagagaa
    14281 tggcgtagcg cccgccggtt tccctgagat gctgaatggc gttgatggcc atgcgcaagc
    14341 cggtgacccc cgggggatgg ccaacggaga tgccaccgcc gtagaggttg tgagcatccc
    14401 tgggaattcc caggtggcgc tccacgtgca ggttcaccac ggcgaacgcc tcgttcacct
    14461 cgaaatagtc gatgtccgaa atgctcagcc cggtccgttc cagcagaagc tgaatggcag
    14521 gcaccggtcc atgtcccatg atccgcggcg gtacgcccac caccgcccag tcgacgacgc
    14581 gggcttccgg ttccaccgac tcggactcga gcgcccctcg gctgcccacg aggatggcgg
    14641 cggcaccgtc cgtgacgccg gacgagttgc cggccgtgac cttcccgccc tcccggaacg
    14701 cgggcttcag acgtgcaaga cgctccatgt cgatgccctc gcggggggat tcgtccacct
    14761 cgaaggtgcg cgtgccgcgt ccgtcgggaa catccacggg cacgatctgc ctggcgagaa
    14821 agcccgactc gatggcagcc agagcccgcg cctggctgga caccgcgtac tcatccatgg
    14881 cttcccggtc gtactgaaat tcctcggtga ggttctcggc ggtttcgccc atgtactcaa
    14941 ggctgaacgg gcaccgataa caccactcga gcatgtcctc caactgctgg ttgccccgga
    15001 cgatgcccca gcgggcggtg gtcaccgcgt acggcacccg gctgaacatc tccgacccgc
    15061 ctgccagggc cagccggctc tgccccgact cgatctgctg acccgcggag aggatggcct
    15121 gcagaccgga ggcacaggcg cgggagacgt ccagggctga cgccatctca ggtatgccgc
    15181 tcttcacgcc taccacgcgg gcagagaaga gcgagtcccg atccgtgggg caggtggtgg
    15241 tgaagaccat gtggtcaacg gaatccgggt tcgcgccgga acgttcgatg caggcgcgcg
    15301 ccacgtggga ggcaagctcc gtcatgggca cgtctttgag cgaaccgccg aaattgccca
    15361 gcgccgagcg ggtgccggcg gcgagataca cgtcgtttgt catcactccc tcccgtcagg
    15421 gcattctagg tcagcgccag agcgcccgcc agaacgttcc cgagatccag attggacgcg
    15481 actaccccac agtgcgacga ttgcggtgca tccaaagagg ggagatctcg agtgactgtc
    15541 atcggcgtgg tgaaggagat ctggcgcttc ccggtcaaat cgatgcaggg gagcacgatc
    15601 gagacgtgca ccatatccag gacgggcatt cccggcgaca ggggctgggc gatgcgcgac
    15661 gaggagcgcc aggaaatcca gtggggcaag cgtcatcccg agctcatgct gtgcagcgcc
    15721 tcctatcgag gtgaacccga gcccgggcag accactccgg tcgacgtcac ttttcccgac
    15781 ggcgagaccc tgggcagcga cgacccgggc attcacgaga agctcagcga gctggttggc
    15841 aggcgggcgt ccctgtggcc ggttcaaccg ccggaggatg aagcctttta ccggcgctac
    15901 aagcccgacg aggagcagtt cgcccgggag atcgctgagg tatttgcccg cgagccgggc
    15961 gagccgatgc ccgacttgag ccagtttccc cccgagattc tggactacgt ctccgtaccg
    16021 ggcacgttct tcgacaacga ggagctgaac ctcgtcacca ccgcctccat agaccatctc
    16081 cgggcaagga accctgctgc caactgggac attcgccgct ttcgaccaaa tttctatgtt
    16141 cagacgccgg acgacatgac gggcttggcc gagaacgagt gggtcggcaa gaccatcagg
    16201 gtcggaaccg ccgtcatgag gatcactgcg gagacaccgc gttgcggcat gacggtgagg
    16261 cctcaggccg agattgagtt cgacaagtcc atactgcgca cgatcgtcag agagtcacac
    16321 cagaatctcg gcgtcggcgc gcactgcatc gaggatggcc ggatcagcgt cggcgacgcg
    16381 ctggtgatcg tggacgcttg aatcatgggc gttctgctcg atcgcccaca acgccctcct
    16441 gctcctggag atcttaaaaa aaacgggccc agccgataag accgctgatc ggccggatgc
    16501 ctaaatccct accggtggca ggcgtccgcg ccgcaagcgg cccggactgc tgctggtcgc
    16561 gccgttgccg gagaccgcac agggttggga tcacatcgcg cctgcgtagg acctttcccg
    16621 ctttcaggtc tcccacattt tcaaaggtac ggtccacgaa tccatccccc tgctgagccg
    16681 gctgcgcagc accctaatcg cggacctgat cggcctgccg gatgaacaca acctggtggt
    16741 cgcgctggcg gaagtggcga ctgccggctc gctgatgctg cggcgctgcc gccctgctcg
    16801 gatccggtcg tgaacgactt cacgccaatc aagggaagtg cgtgacgcgg gtcacagttt
    16861 gggcaggagg gccgcggcgg gcccgccgaa aagcaaggaa aacattacat ctgcgcaacc
    16921 aaccgcccgc aaaacccacc ccatcgccca agtaccatgc tagagattca tttctcatga
    16981 aaagcaaccc cagcagtaac aaggagaacc ccatggcacg cgaacccctt ggccgcaccc
    17041 tggccatgct gccgctcctg gtggccatga ccgctaccgc agccgaccgg caggatctca
    17101 gaacgaatgc ccacgccatc agcacccttc acaagcagac cgaccaaggg ggcagcccca
    17161 actaccacgc cgctttcggc ctcgggcagc aggaaggcct cacagccgtc agcgccacca
    17221 ccgacgcaaa caacgtcacc cacacccgtt accgtcagac cctgcgaggg gtgccggtgt
    17281 ggggcgagca gatcatcatc agccgggagg cttccggggc agtgcgcggc gcacggggcg
    17341 gcctgatcga ggggctgtca gcgcaccagg tgcccgcacg cccgacggtc gccggggcgg
    17401 cggcgttgag cgccaccaag aaccgcatcg cggccggcat gtcacagaac cccgtcttcg
    17461 agcgggagag cgccgagctg gtgatctatc ttcacgacgg cacaccccgc ctgagctggg
    17521 cggtgagctt ttttgccgac gtgcccggcg gcggcgagcc gacccggccg acggtgctct
    17581 tagacgccac gaccggcgaa gtcatcttcg agtatgaggg cctgacccac gctgaaggca
    17641 cggggcccgg cggtaatgca aaggtgggcg gctaccacta cggttccagc gagcctgcca
    17701 tggacgttgc cgccagcggc accacctaca ccatgaacaa cgccaacctg aaaacggtga
    17761 acctcaacca cggaacctcg ggctctacgg cctacagcta tcaaggcccg gagaacgtgc
    17821 acaaggaaat caacggcgct tacagcccgc tgaacgacgc ccacttcttt ggcggcgtgg
    17881 tctacgacat gtacaacgcc tgggtgggca cgccgccgct gacctttcag ctgaccatgc
    17941 gcgtgcacta cggcaacaac tacgagaacg ccttctggaa cggctcagcg atgaccttcg
    18001 gcgacggtgc cagcaccttc taccccctgg tgagcctgga cgtctcggca cacgaggtca
    18061 gccacggctt taccgagcag aactcgaacc tggtctactc cggacagtcg ggcggcatca
    18121 acgaagcgtt ctccgacatg tccggcgagg cggccgagtt ctacatgcgc gggtcgaatg
    18181 actggctggt cggcgcagac atcttcaaga gcccaagcgg cgccctgcgc tacatgtacg
    18241 accccaccct ggatggccgc tccatcggca gcgccaacga ctacgtcaac ggcatggacg
    18301 ttcactactc cagcggcgtg ttcaacaagg ccttctacct gctcgcaacc acaggcggat
    18361 ggggcgtcca gaaggcgttc cagacgttcg cccgtgcaaa ccagctttac tggtcctccg
    18421 gagaaacgtt ccgcaccgcc gccatgggcg tgcaggatgc ggcctcggat ctgggcttct
    18481 ccactgccga cgtcgaggct gccttcagcg ccgtggacgt gaatctgggt gacgtgtgcc
    18541 cggccgagca ggtcgcagag ctgggcaacg gcgcgagcag cggggagttc ggcgccagcg
    18601 ccggcgagtg gcgctgcttc aggctgagcg tcaccgcagg cgggaccgat gcctccttcg
    18661 tgctggcaaa gacggccaag ggtaggggcg gcgacgccga tctctacgtc cagcacgcag
    18721 cgctgccgga caccgttcgc tacgactgcc gctcggtgag cagcaccagc agcgagagct
    18781 gcgccatcgc cacgcctctc gaaggcgact ggtacgtggg cgtctacgcc tacagctcct
    18841 accccggcgt gacgctcacc ggcagcttta ccgaccccag cgggtcgggc ggcggtggag
    18901 gagacggggg cggatcgggg tcgaccgacg ctgactccga cggctacacg tcggacgtgg
    18961 actgcgacga caacaacccg gacgtgcacc ccgaccacaa cgacaccaag ggccgctggg
    19021 ggcgggacgg tgtcgacaac gactgcaacg gcaccatcga cggctgacgc aggtcacacg
    19081 actacgggcg cccgcgggcg cccgattttt tccatcagcc tgccgacgcc gactctccgc
    19141 ttgcgtccgg cgccagattc ttgggcggcg ggatgccgag cgcgttcaga ttgcccacgg
    19201 tgatggccac cgccatggtg aagacgatgg gcttcaacgt gccgtcgaag aacactccgg
    19261 tgaccacgcc gagcacgctg ccgaagagga agacggccac gttcatcagc gacaccgcgc
    19321 tgccgctcag ccgatcgaag taggacaggt agaggccgga cgcggacggg ttgatcatcc
    19381 cgttggcgcc gatgacgacg gacagcaagc cggtcagcgt ccagatgttc agatcgaaca
    19441 accacgagcc cagggcaagc atcaccacgg ccagaaactg cagcccgcgc gccgcgcgcc
    19501 agagacggtc tggcgcaacc cgatggatca gccgggtagt gatcaatgtg aacaccatca
    19561 tcatcaggat gttcacgccg aaatagacga cgaaccactc ctcggcaatg ccgaagtatt
    19621 ccaggtagat gaacgaggag ttggtgatga acacgaacgt cccggacatg agaaggccct
    19681 gggtgaggat gtagcgcgcc ggcacaagcc tgccctcgac ccgccgggtg atcacctcgg
    19741 cgtactgggg caaaatccgg ccgagctcga gcgtgcccgt tcgcatcgaa gcggtttccg
    19801 gcaccggcag gaaggcgacg agcacggcca ggccgtacag ggcgaggaag atgaagatgc
    19861 tcggccagcc gaagctcagc agcgcagcgc cgatggcggg cgccgccagg ggcgcgccga
    19921 gcatgacaag catgaccacg ggaaagcgct tggccgcctc catgggctcg taggcgtcgc
    19981 gcaccatggc catgcagatc accgtggcga acccgccgcc aacggcctgc aacgcccgca
    20041 gccagaggat gacttctatg gattcggcca aggcgatgag cacggacgcg gcagagaaga
    20101 cgactaggcc cagcagcccg atgcggcgc
//
LOCUS       DBNHLM010000117         5290 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_435029, whole genome shotgun sequence.
ACCESSION   DBNHLM010000117 DBNHLM010000000
VERSION     DBNHLM010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5290)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_435029"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="AC16QF_05505"
     CDS             complement(<1..1205)
                     /locus_tag="AC16QF_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR6448043.1"
                     /translation="MTPRRIKGLCSPLGWILLVLIAGCARAPAPVPEPEAGRPGNVVV
                     SGVPAIPAGAAHGLMRYRNVRSAYLMGWADDGLVIATRFGETTQLHRLKTPLGAREQI
                     TFFSEPVLAAFLPTGAGALGAPGFVLSRDAGGSEHYQLFLFDWSTRHARMVSNGRARY
                     TNVLWSNRGDQYAYTTTERDGRRWDIHLQDLAGGIKVALETSDGGWRAVDWSPGDDRL
                     LLIKYVSATESYLYELELASGSLRPLLDESLAVAIGGARYAGNGESVYFTADLGAEFM
                     RLHLLELATNSVEVLTADVPWDVTEIAVTSDGAHLAMVTNEGGVSRLGVLRLPDHSPL
                     ALPEVPAGTIYGLRLSPDGRQLGFTVNAPTAPGDVYSIDLVGRTLTRWTRSEVGGLDP
                     SAFAAPSLV"
     gene            complement(1218..1721)
                     /locus_tag="AC16QF_05510"
     CDS             complement(1218..1721)
                     /locus_tag="AC16QF_05510"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR6448044.1"
                     /translation="MHVALIWAMARNRVIGRDNRLPWRLPRDMQFFVATTMGKPVIMG
                     RRTFESMKAPLAGRTNIVVTSDTGYRREGVLVAHSLEAALDLAQAQCRADDVEEAMVI
                     GGAEVYRVALPIATRLYVTLIDADVEGDTFFPEIDLDAWREVSREAFPADAGNGLPFS
                     IVVFERP"
     gene            complement(1728..2522)
                     /locus_tag="AC16QF_05515"
     CDS             complement(1728..2522)
                     /locus_tag="AC16QF_05515"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR6448045.1"
                     /translation="MKQYLDLMRQVRDTGVFKDDRTGTGTYSIFGHQMRFDLSESFPL
                     VTTKKVVLRLIIHELLWFLQGSTNIKYLTDHNVHIWDEWADEEGNLGPVYGSQWRSWP
                     TPDGGTIDQIAAVVQSLRTNPNSRRHIVSAWNVAEVDSMALPPCHTMFQFYVAQGRLS
                     CQLYQRSADIFLGVPFNIASYALLTMMIAQVCDLQPGEFIHTFGDAHLYANHLEQADE
                     QLSRVPLPLPTVTLNPEVRDIFGFRYEDFTLTNYRCHGTIKAPIAV"
     gene            complement(2629..3462)
                     /gene="lgt"
                     /locus_tag="AC16QF_05520"
     CDS             complement(2629..3462)
                     /gene="lgt"
                     /locus_tag="AC16QF_05520"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019485279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR6448046.1"
                     /translation="MHYPDIDPVIFAIGPLAIRWYGVAYLCAFAMCWWLGVVRAPAKG
                     WTREEVADIVFYGAVGAVLGGRVGYVLFYGFEELIRDPLFLIRIWDGGMSFHGGLLGT
                     LIALWWFGNRTHRSFLQVSDFVAPLVPVGLGFGRLGNFANTELPGRATDSVFGVIYPC
                     SAESIRSINPLCTGQWEAFARHPSPLYQAFAEGVVLFALVWLVSGRSSRTGVVSGTFL
                     IGYGVLRFVTEFFRQPDAHLQFEALEWLTMGQRLSIPMVIIGILLLFWPRLRGGGETR
                     G"
     gene            3545..4291
                     /locus_tag="AC16QF_05525"
     CDS             3545..4291
                     /locus_tag="AC16QF_05525"
                     /inference="COORDINATES: protein motif:HMM:NF017551.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007030 - Golgi organization [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HIR6448047.1"
                     /translation="MCLVLIAYQPESPFPLVVAANRDELYARPARSAHYWADRPHLLA
                     GRDDSAGGTWLGVTRGGRFATVTNFAEEVAEDAPRSRGELPTAFLDSDVSAHDYAHHL
                     DGPSYRGFNLLVWDGDALVYTSNRGTTQDLAPGIYGLANAELGATWPKVTSGVGALRR
                     TLSSGPTVDDLLGLLQDDTQPPDSELPSRGRTIELERQVAPRFIRGDDYGTRASTAVI
                     IGADGIRFAEQEFGPQGVPGRRSDFEVARA"
     gene            complement(4437..5290)
                     /locus_tag="AC16QF_05530"
                     /pseudo
     CDS             complement(4437..5290)
                     /locus_tag="AC16QF_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007252748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
BASE COUNT          982 a         1780 c         1680 g          848 t
ORIGIN      
        1 tgcaccaggc tcggcgcggc aaaggccgac ggatccaaac cgcccacctc gcttcgggtc
       61 catcgggtaa gcgttcgccc aacgaggtca atgctgtaga cgtcgccggg ggcggtgggc
      121 gcattgaccg tgaagcccag ctgccggccg tcgggcgaca atcgcagccc gtagatggtt
      181 cccgcgggca cttccggcag cgctaggggc gagtggtcgg gcaaccgcaa aacccccagc
      241 cgcgagaccc cgccctcgtt ggtcaccatg gccaggtggg ccccatccga ggtaacggcg
      301 atctccgtga cgtcccaggg tacgtcagca gtcagcacct cgacactgtt ggtcgcgagc
      361 tcgagaagat gcagacgcat gaactcggcg cccaggtcgg ccgtgaagta gacgctttcg
      421 ccgttccccg cgtacctggc gccgccaatg gccacggcaa ggctttcgtc cagcagcggt
      481 ctcaagctgc ccgatgcgag ttcgagctcg tacagataag actcggtcgc cgaaacgtac
      541 ttgatgagca gcaggcgatc gtccccgggg gaccagtcta ccgctcgcca gcctccatca
      601 gaggtttcca gcgcgacctt tattcccccg gcaagatcct ggaggtggat gtcccagcgg
      661 cggccgtccc gctccgtggt ggtgtaagca tactggtcgc ctcggttgga ccagagcacg
      721 ttggtgtagc gggcccggcc gttgctgacc atgcgcgcat gacgcgtaga ccagtcgaag
      781 agaaacagct gatagtgctc tgagcccccc gcgtcgcgac tcaagacaaa gcccggagcg
      841 cccaaagcgc cagcgcccgt gggaagaaag gccgccagga ccggttccga gaagaacgta
      901 atctgctccc gcgcgcccag cggcgtcttc agccgatgca gctgagtggt ttcgccgaag
      961 cgggtggcga tgaccagtcc gtcatcggcc cagcccatga ggtaggcaga tcggacgttc
     1021 cggtaacgca tgagcccatg tgccgcgccc gcgggtatgg ccggaacgcc cgacaccacc
     1081 acgttgccgg gccgcccagc ctcaggttcc ggaaccggcg ctggggcgcg cgcgcacccc
     1141 gctatcagga cgagcagaat ccagcccaac ggcgagcaaa ggcccttgat acgacgcggc
     1201 gtcacccggc ccgccaccta gggccgctcg aagacgacga tggaaaacgg cagcccgttg
     1261 ccggcgtccg cgggaaacgc ttcccggctc acctcgcgcc atgcgtccag gtcgatctcc
     1321 ggaaagaagg tgtcgccttc cacgtccgca tcgatcaacg ttacgtagag ccgggtagca
     1381 atcggcagcg ccactcgata gacctccgcg ccgccgatga ccatggcctc ttcgacgtca
     1441 tccgcccggc actgggcctg cgccaggtcg agggcggcct ccaggctgtg ggcaaccagc
     1501 actccctcgc gccgataacc ggtatcgctg gtgacgacga tgttggtgcg cccggccagc
     1561 ggcgccttca tggactcgaa cgtccgccga cccatgatca ccggtttgcc cattgtcgtg
     1621 gccacgaaaa actgcatgtc ccggggcagg cgccatggaa gccggttgtc acggccgata
     1681 acgcggttgc gcgccatggc ccagatcagc gcgacgtgca ccgcgcgtca gaccgcaatc
     1741 ggcgctttga tggtgccgtg acagcggtag ttcgtgaggg tgaagtcctc gtagcggaaa
     1801 ccgaaaatgt cccgcacttc cggattcagc gtgaccgtgg gcagcggcag cggaacacgc
     1861 gagagctgct cgtccgcctg ctcgagatga ttggcgtaga gatgcgcgtc accgaaggta
     1921 tgaatgaact cgccgggctg cagatcacag acctgggcga tcatcatggt gagcaacgca
     1981 taggaggcga tgttgaaggg aacacccagg aagatgtcgg cgctgcgctg gtaaagctgg
     2041 caggaaagcc ggccctgggc cacgtagaac tgaaacatgg tgtggcaggg cggcagcgcc
     2101 atgctgtcca cctccgcgac gttccaggca ctcacgatgt ggcgccgaga attggggttg
     2161 gtgcgaagag actgcacgac ggcggcaatc tgatcgatgg tgcctccgtc cggcgtcggc
     2221 caggaacgcc attgagatcc gtagacgggt cccaggttgc cctcttcgtc cgcccactcg
     2281 tcccagatgt gcacgttgtg atcggtgagg tacttgatgt tggtgctgcc ctggagaaac
     2341 cagagcagct cgtggatgat cagcctgagc accaccttct tcgtcgtcac cagcggaaag
     2401 ctctcgctca gatcgaagcg catctggtga ccgaagatgc tgtacgttcc cgtcccggtg
     2461 cggtcgtcct tgaagacgcc cgtatcgcgc acttggcgca tcaaatcgag atactgtttc
     2521 actggcttcc gttggctgct cgtcccgagt tcggccgcgc tcgcggcccc gtgattaccg
     2581 gacccgctgt ggcgatcggg gccagtgccg gctgttggcg ctcgcttgtc agccccgcgt
     2641 ttcgccccct ccacgcaagc ggggccagaa cagaagcaga ataccaatga tgaccatcgg
     2701 tatcgagagc cgctggccca tggtcagcca ctccagcgct tcgaactgga ggtgggcatc
     2761 cggctggcgg aagaactcgg tgacgaacct caaaaccccg tagccgatca gaaaggtgcc
     2821 cgacaccacc cccgtcctgc tggaccgccc ggagaccaac cacaccagag cgaacagcac
     2881 gacgccctcg gcaaaggcct ggtaaagcgg cgaagggtgg cgtgcaaagg cctcccactg
     2941 tcctgtgcac agcgggttga tgctgcggat ggactccgcc gagcagggat agatcacgcc
     3001 gaagacggag tccgtggcgc gaccgggcag ctcggtgttg gcgaagttgc ccagacgtcc
     3061 gaatcccaga ccgacgggca ccagcggcgc cacgaagtcg ctgacctgca ggaagctccg
     3121 gtgcgtccgg ttcccgaacc accacagcgc gatcagggtg cccagcagcc cgccgtggaa
     3181 cgacatcccg ccgtcccaga tgcggatcaa aaagagcggg tcccggatca gctcctcgaa
     3241 cccgtagaac agcacgtacc cgacgcggcc ccccagcacg gcgcccaccg cgccgtaaaa
     3301 gacgatgtcg gccacctcct cccgagtcca ccccttcgcc ggcgcccgca cgaccccgag
     3361 ccaccagcac atggcaaagg cgcagaggta agccaccccg taccagcgga tggccagggg
     3421 cccgatggcg aagatgacgg ggtcgatgtc aggatagtgc aggacaacct ccgcaggccg
     3481 ggtctgttac actgaaattt cttgccacgc cgttccattc cgaccagcag ccgcgcgaaa
     3541 gagcatgtgt ctcgtcttaa tcgcctacca gcccgagtcg ccctttcccc tggtcgtagc
     3601 ggccaaccgt gacgaactat acgcgcgacc cgcgcgcagc gcacattatt gggccgacag
     3661 gccccatctt ctggccggcc gggacgatag cgccggcggc acctggctcg gcgtcacacg
     3721 aggcggccgc ttcgccacgg tcaccaactt cgccgaggaa gtagccgagg acgcgccccg
     3781 ctcccgcggc gagctgccga ccgcctttct tgacagcgac gtctcggcac acgactacgc
     3841 acaccacctc gacgggccca gctaccgcgg cttcaacctg ctcgtctggg acggggacgc
     3901 cctggtctac acctccaacc ggggcaccac ccaggatctg gcgcctggca tctacggcct
     3961 ggcgaacgcg gagctcgggg cgacctggcc gaaggtgacg agcggggtcg gtgcgctccg
     4021 gcgaacgctc agctcgggcc cgacggtcga cgacttgctt ggcctgctgc aggacgatac
     4081 ccagccaccg gattcggagc tgccgagccg ggggcgcacg atcgagctcg aacgacaggt
     4141 agccccgaga ttcatccggg gtgatgacta cggaaccaga gcgagcacgg ccgtcatcat
     4201 cggcgcggac ggcatccgct tcgccgagca ggagtttggt ccccagggcg tgcccggcag
     4261 gcgctcggac ttcgaggtcg cgcgtgcgtg aggtgcgtgt cagtgatggt agctgggaac
     4321 gacccggccc aggcccgagt cgatcagcca gtcctgcatg aactgctgaa tctcctcagc
     4381 ggtatccatg tgcaggacgt tgaccagcat gcgacgggca tctgagcgtt tgacgttgcg
     4441 cagggtccat ttcactttcg gcaggttggt ggcgttcatg gacagcgcgt cgtagcccat
     4501 cgccatcagc agcaccgctc cggccggggt gcccgccagc tctccgcaga tgcccacgac
     4561 cttctcttcg gcgtgagccg cccgggccac ctcccgaagc gcttgcagaa ccgccggatg
     4621 catctccttg taaagctggg cgacccgtgg gttgttgcgg tcgacggcaa gcatgtactg
     4681 ggtcagatcg ttcgatccta ctgccaggaa gtccaccata cgggccagct ggcgcgcctg
     4741 gtatacggcg gcgggcacct cgatcatcac ccccaccagg ggttgcttca ccacgatccc
     4801 ctcttcgatg acctccacgt aggccctctc caccagcgcc ttggcttcct cgacctcgga
     4861 gagcgaggag accatgggca gcatgatccg cagcaggcat tccaggccgg cgttcgcctt
     4921 gatcatggcg cgaacctgca ccaggaagat ttccgggtgg tccagcgtga cccggatgcc
     4981 tcgccagccg aggaaagggt tgtcctcgtg gatggggaag tagggcagcg atttgtcccc
     5041 acccacatcc agcgtgcgca tggtcacggg gcgcggatcg aaagcctcca tgtgttcccg
     5101 gtaaatggca cgctgctcct cctcgctggg gaagcgctcc ttctccatga agggcacctc
     5161 ggtgcggaac aggccgatcc cctccgcgcc gcggtccaga gagcgggtga tgtcaccggt
     5221 gagcccgatg ttgacccaaa gccccaccgg ccagccgtcc ttggtgacgc tgggcagctc
     5281 ccgcaggtcc
//
LOCUS       DBNHLM010000118         4351 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1453040, whole genome shotgun sequence.
ACCESSION   DBNHLM010000118 DBNHLM010000000
VERSION     DBNHLM010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1453040"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="AC16QF_05535"
     CDS             complement(<1..574)
                     /locus_tag="AC16QF_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010449010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6448048.1"
                     /translation="MRKSTPIRRSSSYTTTPEAKDLIQALLSALNTVLLGKETQVKLA
                     LGCLFARGHLLIEDLPGMGKTLLSHALAKVLGLSYNRIQFTSDVLPGDIIGTSIFERD
                     TGGFRFIEGPLFAQLVLADEINRATPKSQSALLEAMEERQVTIEGKTRSLPEPFFVIA
                     TQNPTTQTGTFPLPESQLDRFLMRIELGYPE"
     gene            complement(522..2048)
                     /gene="purF"
                     /locus_tag="AC16QF_05540"
     CDS             complement(522..2048)
                     /gene="purF"
                     /locus_tag="AC16QF_05540"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011060388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR6448049.1"
                     /translation="MCGIVGMVGTQAINQQLYDALTVLQHRGQDAAGMVTSDGNRVHL
                     RKGNGLVRDVFHQQHMQRLVGNVGIGHCRYPTAGSASSAEAQPMYVNSPYGIALAHNG
                     NLTNSESLQADLFREDLRHVNTDSDSEVLLNVFAHELQVQRALQPEPEHIFAAIEGLH
                     RRCRGAYAAIAMIIGVGVVAFRDPCGIRPLVFGRRENGAGFEYMVASESVALDILGFE
                     RIRDVAPGEAVFITNEGDLHTRVCAEQTSLSPCIFEYVYLARPDSIMDEVSVYKCRLR
                     MGEYLAQRILERYAGNDHDIDVVVPIPDTGRTAALPLAYQLNVKYREGFIKNRYIGRT
                     FIMPGQSQRRKSVRQKLNAVDLELRGKNVLLVEDSIVRGTTMQHVIQMARDAGAKKVY
                     MASAAPPIRFPNVYGIDMPTPKEFVAYDRTVEEVREFIGADWLVYQELEDLVASAREG
                     NPTISQFECSVFDGHYVTGDVDAGYLERISLHRSDQMKQRRDAEINADQTVIELHNHA
                     "
     gene            complement(2055..2552)
                     /locus_tag="AC16QF_05545"
     CDS             complement(2055..2552)
                     /locus_tag="AC16QF_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR6448050.1"
                     /translation="MATPDIVILIIILTSAAIGFVRGLLKELLSLASWIAAFILALYF
                     APTVEQWFEGGISEESVRLAIAFILIFAVTLIIGGIIQWLVRKLVETTGLTGTDRFLG
                     LLFGSARGVLVCIIGLIAIRPFAADADWWGESDFSPALLEFEQDVLELMGRAHDVFED
                     VSEGR"
     gene            complement(2552..3079)
                     /locus_tag="AC16QF_05550"
     CDS             complement(2552..3079)
                     /locus_tag="AC16QF_05550"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR6448051.1"
                     /translation="MDEQTRYRVTGSLFLLALAIICLPMLFDAEGVPSIQIEPMAPPK
                     PAPEVTPLGAVAPASDFIEKVDEFRAQVDDEGFLTASGTRFGEPILSEPGAGTSAWAV
                     QLASLADQDNARDLRSQLREQGYEAFISTVKSGSEVLNRVAVGPILNEIDAEALRAEL
                     SQLYDVDARLMAFAN"
     gene            3824..>4351
                     /locus_tag="AC16QF_05555"
     CDS             3824..>4351
                     /locus_tag="AC16QF_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB C-terminal
                     domain-containing protein"
                     /protein_id="HIR6448052.1"
                     /translation="MSRLAFFFSAAFLLAAVAFLLGLYSGATKSQPYSLIYRIVTDFK
                     SVLSERDEILGTRPVHWTSPRRYEGSGVTMNQIPSGEREFIFLQGLFDNQLQLRLIRR
                     NGEIINSWALEPRSLTNAVTHIRNPPPRDWGFDSHGAAVLEDGSVIFNLDHVGLVKLD
                     RCGKRQWLVEAPTHHS"
BASE COUNT          871 a         1318 c         1308 g          854 t
ORIGIN      
        1 gttccgggta cccgagctcg atacgcatca ggaagcggtc cagctgagac tcgggcagcg
       61 ggaaggtgcc cgtctgagtg gtggggttct gagtcgcgat cacgaagaac ggttcgggca
      121 gcgaccgggt cttaccctcg atggtgacct gccgctcctc catggcctct aggagcgcgc
      181 tctggctctt gggcgtcgcg cggttgatct cgtctgcaag gaccagctgg gcaaagagag
      241 gcccctcgat gaaacgaaac ccgccggtgt cccgttcgaa gatggacgtt ccgatgatgt
      301 cgcccggaag cacgtcactg gtgaactgaa tgcggttgta gctcaacccg agcaccttcg
      361 cgagcgcgtg ggagagcagc gtcttgccca tcccgggcag gtcctcgatg agcagatgac
      421 cccgggcaaa gaggcagccg agcgccagct tgacctgggt ctctttgccc agaaggacgg
      481 tgttgagagc ggacaggagg gcctggatca ggtccttggc ctcaggcgtg gttgtgtagc
      541 tcgatgaccg tctgatcggc gttgatttcc gcatcccggc gctgtttcat ctggtcactt
      601 cgatgcaacg atattctctc cagatagccg gcgtcgacgt caccggtcac ataatggcca
      661 tcgaagaccg agcattcgaa ctgggagatg gtcggattgc cctcccgggc gctcgccacc
      721 agatcttcca gttcctggta aacgagccag tcggcgccga tgaactcgcg gacctcctcg
      781 acggtccggt cgtaggcgac gaactcctta ggcgtcggca tgtcgatgcc gtaaacgttt
      841 ggaaaccgga tgggcggtgc cgcagatgcc atgtagacct ttttcgcccc ggcatcccgc
      901 gccatctgga tgacgtgctg catggtggtt ccgcgaacga tggagtcctc caccagcagc
      961 acgtttttgc cacgcagctc gagatccacg gcgttcagct tctggcgtac ggatttccgg
     1021 cgctgactct gacccggcat gatgaacgtc cgcccgatgt agcggttctt gatgaaaccc
     1081 tcccgatact tgacgttaag ctggtaggcg agagggagag cggccgtccg ccccgtatcc
     1141 ggaatcggca cgaccacgtc gatgtcgtga tcgttcccgg cataccgctc cagtatccgc
     1201 tgggcaaggt actcgcccat cctcaggcga cacttgtaca cggacacctc atccatgatg
     1261 gagtccggtc gcgcgagata gacgtactcg aagatgcacg gagacagcga cgtctgctcc
     1321 gcgcagaccc gggtgtgaag atcaccctcg ttggtaatga acaccgcctc gccgggcgcg
     1381 acgtcacgga tccgctcaaa gccgagaatg tccagggcca cgctttccga ggcgaccatg
     1441 tactcgaacc cggccccgtt ctcacgccgg ccaaacacca gcggccggat gccgcaaggg
     1501 tcgcgaaacg caaccacgcc gacgccgatg atcatggcga tggcggcgta agcgccccga
     1561 cagcgccggt ggagaccttc gatggctgcg aagatgtgct cgggctccgg ctgaagggct
     1621 cgctggactt gcagctcgtg agcgaacacg ttcaacagaa cctctgaatc gctgtccgtg
     1681 ttgacgtggc gaaggtcctc gcggaacaga tcggcctgca ggctttccga gttggtcagg
     1741 ttcccgttgt gagcaagggc aatgccataa ggcgagttga cgtacatggg ctgcgcttcc
     1801 gccgagctcg ccgagccagc cgtggggtag cggcagtgtc caatgccgac gttgcccacc
     1861 agacgctgca tgtgctgctg gtgaaagacg tcgcgaacga ggccgttacc cttgcgcaga
     1921 tgcacgcggt tgccatcgct ggtgaccatg cctgccgcgt cctgtccacg gtgctgaagc
     1981 accgtgagcg catcgtagag ctgctggttg atggcctggg tgccgaccat tccgactatt
     2041 ccgcacatgt ctgctcagcg cccctcacta acatcttcga atacgtcatg agctctgccc
     2101 atcagttcga gcacgtcctg ctcgaactcg agcagcgcgg gcgagaaatc ggactcaccc
     2161 caccagtcgg catcggcagc gaacggtcgg atcgcgatga gcccgatgat acagaccagc
     2221 acgccgcgcg cgctgccgaa cagcaaaccg aggaagcggt cggtgccggt gagtcccgtc
     2281 gtctccacca gcttacgcac cagccactga atgatcccac cgatgatgag ggtgacggca
     2341 aagatcagta tgaacgcgat ggccagtcgc accgactcct ctgagatgcc gccttcgaac
     2401 cattgctcga cagtgggggc gaagtacagg gcgaggataa aggctgcaat ccaggatgca
     2461 agcgaaagca gctctttgag gagaccacgg acgaacccga tcgcggcgga ggtgagaatg
     2521 atgatcagaa tgacgatgtc gggagtagcc atcagttggc aaacgccatc agccgagcgt
     2581 ccacatcgta gagctgggac agctccgccc gcaacgcctc ggcgtcgatc tcgttgagaa
     2641 tggggcccac ggccacgcgg ttgagcacct cggaaccact cttgacggta gaaatgaaag
     2701 cttcgtagcc ctgctcgcga agctgcgagc gaaggtctct agcgttgtcc tgatcggcaa
     2761 ggctcgccag ctgcaccgcc caggccgacg tgccggcacc cggctccgac aggatcggct
     2821 ctccgaagcg ggtcccgctg gcggtgagga acccttcatc atcgacttga gcacggaact
     2881 cgtccacctt ctcgatgaag tcgctggccg gcgccaccgc ccccagcggc gttacttccg
     2941 gcgccggctt gggcggcgcc atcggctcga tctgaatgct cggcacgccc tcggcatcga
     3001 acagcatcgg cagacagatg atcgccagcg cgagcaggaa caggcttccc gtcacgcgat
     3061 accgggtttg ctcgtccact agctggacat cctgttggtc agcactctgc taagccccta
     3121 aaagcaatat tttcttgggc cggctcccta ccggacctac ccgtttcagc aacccgcgaa
     3181 ttgccgttgg gtacagcacg atatccgaat ggatgcgagg gtgggcacac caagtggagg
     3241 ggaatttgcc atctatagag gtccctgagt ttacccagcg atcaatcagc ccccaaaagc
     3301 ggccggtgcc ggggatgaca gccccgacgt ggcctaccca caatgtcatg cgcttgaggt
     3361 gagccgacat gagtgacatc acgttaacgc aggcgaacga cctgccgaaa gaccttcggg
     3421 ctggagcagc agtattgaat agtaagaata gcccacgatc taccaagggc gtagccgcct
     3481 ttgaccttgg gctatgcatt gtagccaacc cgaccggcaa agcgagcttt gcctacaggg
     3541 ctaatatcag cggcgagccg aagcccgagt tccctaaccg ctcgagattt ccatctcctc
     3601 ttgctgggag ctgaagtcga cttcggagtt cgggactgtc tataatctcg caagctcggc
     3661 ctagcttcgt ctttttcgta gcctttgtgg ggttcgtcct ggagctttgc cactcggctg
     3721 gacataaagt tgttggctgt cgcatcggac acataaggcg cgacgagcgt tacacttttc
     3781 gggtgcgtca agtggtgaat cagttctcca aaagctgtca cttgtgtctc gcctagcttt
     3841 cttcttctca gctgcttttc tgttagccgc agtcgcgttt ctgctcggcc tctacagcgg
     3901 tgcgacgaag tctcagccgt actcgctcat ctacaggatt gtgacagact tcaagagcgt
     3961 tctttccgaa cgcgatgaaa tcctcggcac gcggcccgtt cactggacca gcccacgccg
     4021 ttacgagggt tccggtgtca cgatgaatca gattccatca ggagaacggg agttcatatt
     4081 cctgcagggc ctgttcgaca atcaattgca gctaaggtta atccgccgta acggggagat
     4141 aatcaactcg tgggctttgg agcccaggtc ccttacgaat gcagtgaccc atatccgcaa
     4201 cccgccaccg cgggattggg ggttcgactc gcatggcgcg gctgtcctag aagatggttc
     4261 ggtgatcttc aacctggatc atgtcgggct ggtgaagctg gaccgctgcg ggaaaaggca
     4321 atggttggtc gaagcgccta cccatcactc t
//
LOCUS       DBNHLM010000119         7750 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1573031, whole genome shotgun sequence.
ACCESSION   DBNHLM010000119 DBNHLM010000000
VERSION     DBNHLM010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1573031"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="AC16QF_05560"
     CDS             <1..229
                     /locus_tag="AC16QF_05560"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6448053.1"
                     /translation="EAGRCLTYSLTEGRPLGMRSYSVTVSVEAAGSGCTLAWQGGFVP
                     EPGADSDAMAENLRRAYVDMSRRLDVLLRAA"
     gene            complement(296..1492)
                     /locus_tag="AC16QF_05565"
     CDS             complement(296..1492)
                     /locus_tag="AC16QF_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6448054.1"
                     /translation="MDHHRPNVTTTIDRSPTRPDKAADTTLKALFLLTAVLMVGYGAV
                     FSLLAEIRDAFGFTPTGIGLIAGSAFAAGFFAQLWLARYADRGYGAIMLRAGLAISAL
                     STLWMAVADTLAAWIAARALLGFGAGCVRPAVRRLALTADPANAGHAMGRLTAYETAG
                     FLLGPVLAALLDRWAGLASTFIVMTGVLALLAPMVWRVSIPGASRPTTGPVIGTLLRN
                     PYMQACLALGIAFWITIGVFEAVWAIFLADLGASQMFIGLTMSLFGIPMIFIAPRAGS
                     LAQHRGPLRVASVSMAVATVCMVLYGFLESLWLLCVPLGIHAIADAYTMPASQLAVGR
                     ASGEGALAAGQGLFGATGMAVGAVTAAGGGVLYQSLGPSGLWWGSAGAMIVCLLISWW
                     RSPRAT"
     gene            1542..2273
                     /locus_tag="AC16QF_05570"
     CDS             1542..2273
                     /locus_tag="AC16QF_05570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6448055.1"
                     /translation="MNDNAIALVTGGATGIGAEVCRQLAAAGARVAICDVNEADGAPL
                     AESLGGEFLFCDVADLASVEGAVAGCCARLGVPDYVHLNAGVMTVGRYDAYLALEDVS
                     VEQYRRIIGVNVDGVFNGIKALLPPMRQKGGAITVTASIAGFAPMAIDPLYGATKAAL
                     ISLVRSVAAANEGGSVRINAICPGVVDTAIVPDAMSGTIPMMPPSAMAAEILDLLSDG
                     PSGEIRVKLNEETPAFHVEPSTLGR"
     gene            2288..2773
                     /locus_tag="AC16QF_05575"
     CDS             2288..2773
                     /locus_tag="AC16QF_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6448056.1"
                     /translation="MNFDDYHAIVRRRYEYALGIDTRDWVLYRSIFTDEITMDFSSYN
                     GRPASGMAADDWVAGCKVLFAGLDATQHVMSNPMVDVDGDNARLRMYMKAEHFLRNDQ
                     GGMDFTIGGYYDDRLVRTDEGWKMQAVTLNVFWSRGNRQIMALAAEIGKGKLEEPSSG
                     S"
     gene            2797..4080
                     /locus_tag="AC16QF_05580"
     CDS             2797..4080
                     /locus_tag="AC16QF_05580"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6448057.1"
                     /translation="MNDPESIANLEARMAWEQERAVPPEGFPALPPLPPGRYTSPAFF
                     DLERRHLWSKVWLYAAHDSQLPEPGSFLLLDIPDAPIFLIRGRDASVRAFYNVCSHRG
                     APLVREASGQVARLRCTYHQWTYDTDGRLIAVMDERDFAEPFDRSCLGLKEVRCEQWG
                     RWIFINEDPEAQPLLEWLDMIAAEFEQFDIERLRPAAIKSYELECNWKLTMDAFLEVY
                     HIQGIHPKTVGPSLDHRGAVMGLLPNGHTRMTCPTRMSRTETADTRGYTDSAGEAMPA
                     IPAGEIARNYHVSHNVFPNLITPVGDTARQFLMFWPLSLTRTRVDVVHFGLDWGEGER
                     PSGWDGLLAFWDVVMAEDLQFLEWQQKAVLSPGFQGYRLSYMERRIYYAHESIDRIIG
                     VENIPEALRAVPMLDAYRERAEDRCADQPSSPSSR"
     gene            complement(4117..4467)
                     /locus_tag="AC16QF_05585"
     CDS             complement(4117..4467)
                     /locus_tag="AC16QF_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448058.1"
                     /translation="MTAAREFYVLVGLMMAAAVPMIVEIRADHGVGVLQVAAQIWTDA
                     LSAFTGDILNRRPVDRATLTGPVLALRLVYCLGPIVFYGLALKLIWSFPLTPVRHARL
                     RERIERRNARRTAR"
     gene            4748..5794
                     /locus_tag="AC16QF_05590"
     CDS             4748..5794
                     /locus_tag="AC16QF_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6448059.1"
                     /translation="MKVSIGIGGAASGGRRDFEDQVAYVVEAEALGVDTVWTAEAWGQ
                     DAISPLAYLAAKTQRIRLGTGIMQISARTPSMTAMTALTMAAISNDRFILGLGASGPQ
                     VVEGLQGQPFKAPLTRMRETVDIIKLAFAGEKLAYEGKYHRLPLPGGEGKALRLAQPG
                     NDSIPIYLATLGPKSLEYTGEAADGWLGTSFTPEHADAHLGHIRAGAEAAGRSLADVD
                     VQVGGTVAFGDDLAALVAPLKRAMAFTLGAMGSPKTNFYNEAYKRGGWSEAAVEVQQL
                     WVSGRREEAAQRVPDEMIVQANLLGDEAMVRQRIRAYRDAGVTTLRVNPMGEGASGRL
                     ETLARVMDMVKALD"
     gene            5825..6154
                     /locus_tag="AC16QF_05595"
     CDS             5825..6154
                     /locus_tag="AC16QF_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 family protein"
                     /protein_id="HIR6448060.1"
                     /translation="MNGTATQAKVIYVLYLAALLNGVTMLIGVVMAYLARGDAPGWLG
                     THYRFQIRTFWMGLLYSMLATMLTPVLIGFFGYLLIAIWLIVRCVKGLKTADREEPME
                     DVETWLF"
     gene            complement(6162..6947)
                     /locus_tag="AC16QF_05600"
     CDS             complement(6162..6947)
                     /locus_tag="AC16QF_05600"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR6448061.1"
                     /translation="MTDPSDLVLLERSGAVATVTLNRPEALNALSAALRDRIAEVFTE
                     IASTPDVEVVILTGAGRAFTVGLDLKELGGEVDPTATTSSRDLSAAIGSLHQPIIGAI
                     NGFAITGGFELALMCDFLIASPQAKFADTHARVGVVPGWGLSQRLPRLIGINRAKELS
                     LTGNYLDAQTACAWGLVNRVVPADELLSTCHRLAEDIVSTDRATRQEIRRIMDAGWGA
                     TLGEGLEIERDANAAHGRSEVRAEKVAARRADIQSRGREQSRD"
     gene            7097..>7750
                     /locus_tag="AC16QF_05605"
     CDS             7097..>7750
                     /locus_tag="AC16QF_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448062.1"
                     /translation="MQFSDYVTSRALHITSDCLRLLSGSSLEDGVLTAGAASNLLRSV
                     GRLESLWQLEQWCVGKAEYRRALARLRGAEQVLHPRAVWFDGVASQLLARTDDERARR
                     AVERARRAFAQTATGPEAVDPALLNRVFQEESAVWRAQASYSGTRDADLVNYGIGRAF
                     NESRRAVARLEEKPASRKRLRRAHCWVGHVAHHLELLQPALSEANDARAWFIGRLEAN
                     "
BASE COUNT         1370 a         2463 c         2655 g         1262 t
ORIGIN      
        1 cgaggccggc cgctgcctca cctacagcct caccgagggt cgtccgctgg gcatgcggag
       61 ctactcggtg accgtgagcg tggaagcggc cggatccggc tgcacgctgg cgtggcaggg
      121 tgggttcgtt ccggagcctg gcgccgattc ggatgccatg gccgaaaacc tgcgccgagc
      181 ctacgtcgac atgtctcggc ggctcgatgt gctgctgcga gcagcgtgac ccgtcgcctg
      241 caagcctgca tcagcggcaa tggcaaagac gagggcgata agttcgatgc cgtgatcagg
      301 tcgctcgagg gctgcgccac caggatatga gcaggcagac gatcatggcc ccggcagacc
      361 cccaccacag cccgctcggg ccgaggctct gatacaagac cccgccgcct gcggcggtca
      421 cggcgccgac ggccatcccg gtggcgccga acaggccctg gcccgctgcc agggccccct
      481 caccgcttgc ccgtccgacc gctagctggc tcgccggcat ggtgtaggcg tcggcgatgg
      541 cgtgaatgcc cagcggaacg catagcagcc acagcgactc cagaaacccg tagagaacca
      601 tgcatacggt tgcgaccgcc atgctgaccg aggccacccg gagaggcccc cggtgctggg
      661 ccagggagcc ggcacggggc gcgatgaaga tcatcgggat cccgaagaga ctcatggtga
      721 gcccgatgaa catctggctg gcccccagat cggcgagaaa tatggcccag accgcttcga
      781 agacgccgat ggtgatccag aacgcgatgc cgagagcaag acatgcctgc atgtaaggat
      841 tgcgcaacag cgtgccgatg acgggacccg ttgtcggtcg gctggccccg ggaatgctca
      901 cgcgccacac catgggcgcg agcagcgcca ggacgcccgt catcacgatg aaggtggacg
      961 ccaggccagc ccagcggtcg agcagcgcgg cgagcacggg gccgagaagg aagccagccg
     1021 tctcgtaggc ggtcaaacgt cccatggcgt ggccggcatt ggcggggtct gcggtgagtg
     1081 ccagacgacg tacggcgggg cggacgcagc cggcgccgaa ccccagcagg gcgcgcgcgg
     1141 caatccacgc agcgagggtg tcggcgacgg ccatccacag ggtgctcagc gccgagatcg
     1201 cgagacctgc ccgcagcatg atcgccccgt aaccgcgatc cgcgtaccgc gcaagccaca
     1261 gctgggcgaa gaaaccggcc gcgaacgcag agcctgcgat cagcccgatg cccgtaggcg
     1321 tgaagccgaa cgcgtcgcgg atttcggcca gcagggaaaa caccgccccg tagcccacca
     1381 tcaggacggc ggtcagaagg aaaagggctt tgagcgtggt atcggctgcc ttgtcaggtc
     1441 tggtaggtga acggtcgatc gtcgtggtaa cgtttggtcg gtgatggtcc atcgaaccgc
     1501 aatctagggc ggaaagcaaa gatcatgcga ggaggggaag ggtgaacgac aacgcgatcg
     1561 cgctggtgac cggaggggca accggtatcg gcgccgaggt gtgcagacag ctcgccgcgg
     1621 cgggagcaag ggtggccatc tgcgacgtca acgaggcgga cggcgcgccg ctggccgagt
     1681 ccctgggcgg cgagtttctg ttctgcgacg tggcggatct ggcgagcgtg gagggagcgg
     1741 tcgccggctg ctgtgcccgg cttggggtgc ccgactacgt acacctgaat gccggcgtca
     1801 tgactgtggg ccgctacgat gcttaccttg cgctcgagga cgtcagcgtg gagcagtacc
     1861 gccggatcat cggcgtcaac gtggacggtg tcttcaacgg tatcaaggcg cttctgcctc
     1921 ccatgaggca gaagggcgga gccatcacgg tgacggcctc cattgccggc ttcgccccga
     1981 tggccatcga tccgctctac ggcgccacca aggccgccct gatcagcctc gtgcgcagcg
     2041 tcgcggcagc caacgaaggc ggcagcgtgc gtatcaacgc catctgcccg ggggtggtgg
     2101 acaccgccat agtgcccgat gcgatgtccg gcacgatacc gatgatgcca ccgtccgcca
     2161 tggcggctga gatactggac ctcctcagcg atggcccctc cggcgagatc cgcgtcaagc
     2221 tcaacgagga gacgcccgcc ttccacgtcg agccgagcac cttgggtcgc tgaacgggca
     2281 ggccatcatg aatttcgacg attaccatgc catcgtccgt cgccgctacg agtacgcgct
     2341 ggggatcgac acgcgggact gggtgctcta tcgcagcatc ttcaccgacg agatcaccat
     2401 ggatttctct tcctacaatg gccgacccgc atccggcatg gcggccgacg actgggtggc
     2461 cggctgtaag gttctgtttg ccgggctcga tgccacccag cacgtgatgt ccaatccgat
     2521 ggtcgacgtc gacggggata atgcccggct gcgcatgtac atgaaggccg agcactttct
     2581 gcgcaacgat cagggcggca tggatttcac catcggcggc tactacgacg accggctggt
     2641 caggacggat gagggctgga agatgcaggc ggtgacgttg aacgtcttct ggagccgcgg
     2701 caatcggcag atcatggcgc tcgcggcgga gattggaaag ggcaagctcg aagaaccgtc
     2761 gtcgggttcg tgacagcgcg ttagggagta cggccgatga acgatccgga gtccattgcg
     2821 aacctcgagg cgcgcatggc ctgggagcag gaacgggcgg ttccgcccga gggctttccg
     2881 gcgttgccgc ctctgccgcc cggccgctac accagcccgg catttttcga tctcgagcgt
     2941 cgtcacctgt ggtcaaaggt ctggctctac gcggcccacg acagccagct gccggagccc
     3001 ggcagctttc tcctgctgga catccccgat gcgccgatct ttctgattcg tggccgcgat
     3061 gcaagcgtcc gcgcgtttta caacgtctgc tcgcatcgcg gagcgccgtt ggtgcgggag
     3121 gcctccggtc aggtggcccg gcttcgctgc acctaccacc agtggacgta cgacacggac
     3181 ggacgactga tcgccgtcat ggacgagcgc gacttcgcgg agcccttcga tcgatcctgc
     3241 ctcggcctga aggaagttcg ctgcgagcag tggggtcggt ggatattcat caacgaggac
     3301 cccgaagcac agccgctgct ggaatggctc gacatgatcg ccgccgagtt cgagcagttc
     3361 gacatcgagc gcctgcgccc ggcggcgatc aagagctacg agctcgagtg caactggaag
     3421 ctgaccatgg atgcgttcct ggaggtctac cacatacagg gcatccaccc gaagaccgtg
     3481 gggccatcgc tcgaccaccg gggcgccgtc atggggctgc ttcccaacgg gcacacgcgc
     3541 atgacttgcc cgacccggat gtcccgcacc gagaccgccg acacgcgcgg atacaccgat
     3601 tcagcaggcg aggcgatgcc tgccattccg gctggcgaga tcgctcgcaa ctatcacgtc
     3661 tcccacaacg tctttccgaa tctcatcacg cccgttggcg acacggccag gcagtttctg
     3721 atgttctggc ccctttcgct cacgcggacc cgggtggacg tggtccattt cggcctggac
     3781 tggggtgagg gcgagcgtcc ctcgggatgg gatggcctgc tcgccttctg ggacgtggtg
     3841 atggccgagg atctgcagtt tctggagtgg cagcagaagg ccgtgctctc gccaggattt
     3901 cagggctacc gcctgagcta catggaacgc cggatctact acgcccacga gtccatcgac
     3961 cggatcatcg gcgtggagaa cattcccgag gcgctgcgcg ccgtgcccat gctggatgcc
     4021 taccgcgagc gcgcggaaga ccgctgcgcg gatcagccgt cgagcccgtc ctcccgttaa
     4081 ccccggggtt cccgtgcccg aggggcccgg tgatccctac cgtgccgtgc gcctggcgtt
     4141 acgccgctcg atccgctccc gcagccgggc gtgccgaacg ggagtgagcg ggaatgacca
     4201 gatgagcttc agcgccagtc cgtagaagac tatgggcccc aggcagtaga cgagccgcag
     4261 ggccagcacc gggccggtca acgtggcgcg gtccacgggt cgcctgttga gaatgtctcc
     4321 cgtgaaggcg ctgagcgcgt ctgtccagat ctgcgcggcc acctggagca cgcccacgcc
     4381 gtggtcggcc cggatctcca cgatcatcgg cacggcggcg gccatcatca gccccaccag
     4441 cacgtagaac tcccgggcag cggtcacccg gctgcgctga tggcaggcgg gcgaccatca
     4501 ggatggtgcc caccagcgcc agagagatac cgagccctcc cgagtaatgg gcggggatcc
     4561 agatcgccag cgggtatccg atcacggcaa gcggcaggcc gaggcagccg tgaatccaga
     4621 tggtgggtct gggcagggga agaactggag acgacgcgtc gtctgccgca gcgctcatgg
     4681 ctttctcgct cgtcgctccg tgagcagggt attatccacg actttcgcgc agtggatgga
     4741 agccggaatg aaggtcagca tcggcatcgg cggggccgcc tctgggggcc gacgtgattt
     4801 cgaagatcag gtcgcctacg tcgtcgaggc ggaagccctc ggcgtggata ccgtctggac
     4861 ggcggaagca tggggccagg acgccatcag ccctcttgcc tacctggcgg cgaaaacgca
     4921 acggatacgg ctcgggacgg ggatcatgca gatcagtgcc cgcacgcctt ccatgaccgc
     4981 catgacggcg ctcaccatgg ccgccatctc caacgaccgt ttcatcctgg gcctgggtgc
     5041 cagcggtccc caggtggtcg aagggctgca gggacaaccg ttcaaagccc cgttgacgcg
     5101 catgcgcgag acggtggaca tcatcaagct ggcgtttgcc ggggagaagc tggcttacga
     5161 aggcaagtat caccgactcc ccctgcccgg cggcgagggc aaggctctgc gcctggccca
     5221 gccgggcaac gacagcattc ccatctatct ggcgaccctc ggaccgaagt ccctggaata
     5281 caccggagaa gccgccgacg gctggctcgg cacgtcgttc acccccgagc atgcggacgc
     5341 ccatcttggc cacatccggg ccggcgccga agcggcagga cgcagcctgg ccgacgtgga
     5401 cgtccaggtg ggcggcaccg tggcgttcgg cgatgacctg gccgcgctgg tggcgccgtt
     5461 gaagcgggca atggcgttta cgctcggggc catgggatcg cccaagacca acttctacaa
     5521 cgaggcatac aagcgagggg gctggtcgga agccgcggtg gaggttcagc agctgtgggt
     5581 cagcggcagg cgcgaagagg ccgcgcagcg ggtgcccgac gagatgatcg tccaggccaa
     5641 ccttctcggt gacgaggcca tggtccgcca gcgcattcgc gcctacaggg acgcgggcgt
     5701 caccacgctg cgggtcaacc cgatgggcga aggcgcatcc ggacggctgg agaccctcgc
     5761 acgggtgatg gacatggtca aagccctcga ttgaaaccat gaagatattc cgggagggac
     5821 ggtcatgaac ggaacggcaa cccaggccaa ggtcatctac gtgctgtatc tggctgcttt
     5881 gctcaacggg gtcaccatgc tgatcggggt ggtgatggcc tacctcgccc ggggcgatgc
     5941 gccgggctgg ctgggcacgc actaccgttt ccagatccgc acgttctgga tggggctgct
     6001 ctactcgatg ctcgccacca tgctgacgcc ggtgctgatc gggttcttcg gctatctgct
     6061 gatcgcgatc tggctcatcg ttcgctgcgt gaaggggctg aagaccgcgg atcgtgagga
     6121 gcccatggaa gacgtggaga cctggctctt ctgaagcggg ttcagtcccg actctgctcg
     6181 cggccgcgtg actgtatgtc ggctctgcgg gcggcgacct tttcggcccg cacctccgag
     6241 cgtccatggg ccgcgttggc gtcccgctcg atctcgagcc cttcgccaag ggtcgctccc
     6301 catcctgcgt ccatgatgcg cctgatttcc tggcgggttg cccggtcggt ggagacgatg
     6361 tcctcggcga gccgatggca cgtcgacaga agctcgtctg ccgggaccac tcgattgacc
     6421 aggccccagg cgcaagccgt ctgcgcatcc aggtagttgc ccgtcagcga aagctccttg
     6481 gcccggttga tgccaatcag ccgcggcaag cgctgggaca ggccccaccc cgggaccacg
     6541 cctaccctgg cgtgggtgtc cgcgaacttc gcctgaggag aggcgatgag gaagtcgcac
     6601 atcagggcga gctcgaagcc cccggtaatg gcgaaaccat tgatcgcacc gatgatgggc
     6661 tggtgcagcg acccgatggc cgcgctgagg tctcgggaag acgtggtggc cgtcgggtcc
     6721 acctcgccgc caagctcttt gaggtccaga ccaacggtaa acgcgcgtcc cgcgccggtg
     6781 agaatcacga cctcgacgtc tggcgtagag gcgatctcgg tgaagacctc ggctattcgg
     6841 tcgcgcagag cggccgacag ggcgttcagc gcttcgggcc ggttcagcgt gaccgtggca
     6901 actgccccgg atcgttcgag aagaacgaga tctgatgggt ccgtcatgtc gctctcgtca
     6961 gacagggtgg cgccattcta gcagagccgg gtggactccc ccgcgcggtc gtttgtacga
     7021 tggggcagac ggcgggccgg gcgtcgtccg taaggtcgtc aggggacgac gtgtggcgcc
     7081 gcggagaaga tgagccatgc aattttccga ctacgtcacc agccgcgcgc tccacatcac
     7141 gagcgactgc ctgcggctgc tttcgggatc ctcgttagag gatggggtcc tgaccgccgg
     7201 tgcggcttcc aatctcctgc gcagcgtcgg tcgcttggag tccctgtggc agctcgagca
     7261 gtggtgcgta ggcaaggcgg agtatcgacg tgccttggcg cggctgaggg gcgcagagca
     7321 ggttctgcat ccgcgggcag tctggttcga tggggtcgcg tctcagctgc ttgccaggac
     7381 ggacgacgag agggcccggc gagcggtgga gcgtgcccgg agggcgtttg cgcagacggc
     7441 caccgggcca gaagcggtcg acccggcgct gctcaatcgc gtgtttcagg aagaatcggc
     7501 ggtatggcgg gcacaggctt cttactctgg gacgcgcgat gccgatctgg tcaattacgg
     7561 gatcggccgg gctttcaacg agtcccgccg agcggttgcg cggttggagg aaaagccggc
     7621 gagcagaaaa cgcctgcgac gcgcccattg ctgggtaggc catgtcgctc accatctgga
     7681 gcttctgcag cccgcgctct ccgaggcgaa cgacgccaga gcctggttca tcggccggct
     7741 cgaggcgaac
//
LOCUS       DBNHLM010000120         4179 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1272654, whole genome shotgun sequence.
ACCESSION   DBNHLM010000120 DBNHLM010000000
VERSION     DBNHLM010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1272654"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1387
                     /locus_tag="AC16QF_05610"
     CDS             14..1387
                     /locus_tag="AC16QF_05610"
                     /inference="COORDINATES: protein motif:HMM:NF013645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GltB/FmdC/FwdC-like GXGXG domain-containing
                     protein"
                     /protein_id="HIR6448063.1"
                     /translation="MSGDSGGTGASPLSSIKYAGIPWELGLAETHQTLVLNNLRSRIV
                     VETDGQLKTGRDVVIAALLGAEEFGFATAPLVALGCIMMRKCHLNTCPVGIATQDPVL
                     RKKFAGQPEHVVNYFFMVAKELRMIMAELGFSTMNDMVGRVDALNTDDAIDHWKARGL
                     DLSEILTRAEEPAEFLGSYAIHEQDHGLEKALDNELMQLAEPAIKRGEKVYHELGIVN
                     TNRVVGGMLSNKIIREVGPDMLPDESIHFKFSGSAGQSFGCWLAKGVTLEVEGDANDY
                     VGKGLSGGRLIIYPPKSSPFKPEEQIIVGNVVLYGATSGECYFRGIAAERFCVRNSGA
                     HAVIEGVGDHGCEYMTGGRVVILGETGRNFGAGMSGGIAYVWDPQDRFRTQCNMEMVD
                     LERLEDSVEIAEVRGMIERHQQYTGSTVAEQVLADWEASLGMFAKVMPVDYKRVLEQR
                     RDEVSAG"
     gene            1390..2853
                     /locus_tag="AC16QF_05615"
     CDS             1390..2853
                     /locus_tag="AC16QF_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HIR6448064.1"
                     /translation="MGKVTGFMEFDRNAAPYRNAGKRLLDFQEIYTDHDEARLTNQGA
                     RCMDCGVPFCQSNDGCPIHNLIPEWNDLVYRHQWREALDRLHKTNNFPEFTGRVCPAP
                     CEGACVLGITDPAVTIKNIEMAIIDRGFEEGWVKPNPPSQRTGKRIAVVGSGPAGLAA
                     ADQLNKAGHTVIVYERADRIGGLLMYGIPNMKLDKAEVVERRLNLLREEGVEFVTNAH
                     IGDGSQGTISGSDLVNDFDAVLLATGATIPRDLPIPGRDLRGVHFAMEFLTANTKSLL
                     DSGHADGAYISAKDKDVIVIGGGDTGTDCIGTSLRHGCRSLVNFELFPKPPEVRGPDN
                     PWPTWPRIFRTDYGHQEAAERFGSDPRVYAISSTEFFGDEAGNLKGVRTVDVTLKDGR
                     FENVPGSEREWPADLVFLSMGFLGPEHLVSDSLGIAYDDRSNYSAEYGRYATSVDKVF
                     AAGDCRQGQSLVVRAINEGREAAREIDRFLMGASQLP"
     gene            complement(2861..3946)
                     /gene="aroB"
                     /locus_tag="AC16QF_05620"
     CDS             complement(2861..3946)
                     /gene="aroB"
                     /locus_tag="AC16QF_05620"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020302367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR6448065.1"
                     /translation="MTLEVDLGDRSYPIHIGDGLLGRGELLRDQVHGGQVAVITNDVV
                     GPLYLPRVAQALAGIDADVYTLPDGEQHKNLESYGAIMDFLMERRHNRSTTLVALGGG
                     VVGDITGFVAATFQRGVAFVQVPTTLLAQVDSSVGGKTAINHPRGKNMIGAFYQPRCV
                     IADTTVLNSLGDRELRAGLAEVLKYGIIADAGFFDWLESNANALLSREPETLAVAIRR
                     SCEIKAAVVAEDERESGIRAILNYGHTFGHAIETLTGYGELLHGEAVAAGMVMAADLS
                     ARQGWLTGTEAKRIKAALEAFGLPVVAPSIEPDDMLSAMSIDKKVVNGRLRLVLARAI
                     GEAAVTEDVDEPALRATLAAGEALCDG"
     gene            complement(3952..>4179)
                     /locus_tag="AC16QF_05625"
     CDS             complement(3952..>4179)
                     /locus_tag="AC16QF_05625"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR6448066.1"
                     /translation="VHLDSPLERLVERTARDNKRPLLQGGDARETLARLQRERAPLYA
                     EIADYTFVTDRQGPKVLARTIERKLREDGLI"
BASE COUNT          784 a         1359 c         1332 g          704 t
ORIGIN      
        1 cgataaagtc ctcattagcg gcgattccgg gggaacgggg gcgtctccct tatcctccat
       61 caaatacgct ggaatacctt gggaattggg tttggccgaa acgcatcaga ccctggtgct
      121 caacaacctg cgcagccgga tcgtggtcga gacagacggg cagctcaaaa ccgggcgcga
      181 tgtcgttatc gccgctctgc tgggggccga ggagtttggt tttgccaccg ccccgttggt
      241 ggcgctcggc tgcatcatga tgcgcaagtg tcatctcaac acctgccctg tcggcatcgc
      301 cacccaggat ccagttcttc gcaagaagtt cgccgggcaa ccggaacacg tggtgaacta
      361 cttcttcatg gtggccaaag agctgcgcat gatcatggcc gagctcggct tctcaacgat
      421 gaacgacatg gtcggccggg tggacgcgct gaacaccgat gacgccatcg atcactggaa
      481 ggccaggggt ctggacctca gcgagattct cacgcgagcc gaggagccgg ccgagttcct
      541 gggctcctac gcgatccacg agcaggacca cgggctggaa aaggcgctgg acaacgagct
      601 catgcagctc gccgagccgg ccatcaagcg cggcgagaag gtctatcacg agctcggcat
      661 cgtcaacacc aaccgcgtgg tgggcggcat gctgtcgaac aagatcatcc gggaagtggg
      721 tccggacatg cttccggacg aatccatcca cttcaagttt tcgggaagcg ccggccagag
      781 cttcggctgc tggctggcca aaggcgtgac gctggaggta gagggagacg ccaacgacta
      841 tgtcggcaaa ggcctctccg gcggccgcct catcatctac ccgccgaagt cgagcccgtt
      901 caagccggaa gagcagatca tcgtcggcaa cgtcgtgctc tacggagcca ccagcggcga
      961 gtgctacttc cgcggcatcg ccgcggagcg tttctgcgtg cgtaacagcg gggcgcacgc
     1021 agtgatcgag ggcgtcggcg accacggctg cgagtacatg accggcgggc gcgtcgtcat
     1081 cctgggcgag acgggacgca acttcggcgc cggcatgagc ggcggcattg cctacgtctg
     1141 ggatccgcaa gacaggttcc ggacccagtg caacatggag atggtggatc tggaacgtct
     1201 agaggactcc gttgagatcg ccgaagtgcg cggcatgatc gagcggcacc agcagtacac
     1261 gggctcgacg gttgccgagc aggtgctcgc ggactgggag gcttcgctgg ggatgttcgc
     1321 caaggtgatg cccgtcgact acaaacgggt gctggaacag cgccgcgacg aagtgagcgc
     1381 gggttgaaca tgggtaaagt taccggattc atggagttcg atcgcaacgc ggcgccgtac
     1441 cggaacgccg gcaaacgtct gctcgatttc caggagatct acaccgatca cgacgaggcg
     1501 cgcctgacca atcagggcgc gcggtgcatg gactgcggcg ttccgttctg ccagtccaac
     1561 gacggttgcc ccattcacaa tctcattccc gaatggaacg acctcgtcta ccgacaccag
     1621 tggcgggaag cgctggaccg gctgcacaag accaacaatt tcccggagtt caccggccgc
     1681 gtctgcccgg cgccctgcga aggggcctgc gtgctgggga tcaccgatcc ggccgtgacc
     1741 atcaagaaca tagagatggc gatcatcgac cgtggtttcg aggaaggatg ggtcaagccc
     1801 aatcctccga gccagcgaac gggcaagcgg atcgccgtgg tcggatccgg ccccgctggc
     1861 cttgcggcag ccgaccagct gaacaaagcc ggccacacgg tcatcgtcta cgagcgggcg
     1921 gaccgcattg gcgggctgct catgtacggc atccccaaca tgaagctgga caaggccgag
     1981 gtcgtcgagc ggcggctgaa cctcctccgt gaggagggcg tcgagttcgt caccaacgcc
     2041 cacattggcg acggcagcca aggcaccatc agcggcagcg acctggtgaa cgatttcgac
     2101 gcggtgctgc ttgccaccgg cgccacgata ccgagagatc tgcccattcc gggccgggac
     2161 ctgcgcggcg ttcacttcgc catggagttt ctcactgcca acaccaaaag cctgctggac
     2221 tcgggccacg cggacggcgc gtacatctcg gcaaaagaca aggacgtcat cgtgatcggg
     2281 ggcggcgata ccggcaccga ctgcatcggc acatcgctgc ggcacggatg ccggagcctg
     2341 gtgaatttcg agctgtttcc aaagccgccg gaagtccggg gcccggacaa cccctggccc
     2401 acctggccga gaattttccg gacagactac gggcaccagg aggccgccga gcgtttcggc
     2461 agcgaccccc gcgtctacgc gatctcttct accgagttct tcggcgacga agccggaaat
     2521 ctgaaagggg tgcggaccgt cgacgtgacg ctcaaggacg gcaggtttga gaacgttccg
     2581 ggatccgaac gggagtggcc ggccgacctg gtgtttctct ccatgggttt tctgggcccc
     2641 gagcacctgg tctccgattc cctcggaatc gcctacgacg accgctccaa ctactcagcc
     2701 gagtacggca ggtacgccac cagcgtcgac aaggtgttcg ccgccggcga ctgccgacag
     2761 ggtcagtccc tggtggtgcg agccatcaac gagggacgcg aggcagcgcg cgagatcgac
     2821 cgcttcctca tgggcgcctc acagctgccc tgacgggcgg tcagccgtca cacagagctt
     2881 cgcccgccgc cagcgtggcc ctcagcgcag gctcgtcgac gtcttcggtg acggccgctt
     2941 ccccgatggc gcgcgcgagc accagtcgca agcggccatt cacgaccttc ttgtcgatgg
     3001 acatcgcgct cagcatgtcg tccggctcga tggaaggtgc caccacgggc aatccgaacg
     3061 cctcgagcgc agctttgatg cgcttggcct ccgtacccgt cagccagccc tgccgagccg
     3121 aaagatcggc cgccatcacc atgcccgccg ccaccgcctc accatgcagg agctcgccgt
     3181 aacccgtcag cgtctcgatg gcgtgaccga aagtgtggcc gtagttcagg atcgcccgga
     3241 tgccggactc ccgttcgtcc tcggccacca cggccgcttt gatctcgcag gaccggcgta
     3301 tcgccacggc aagcgtttcc ggctccctgc tgagcagcgc gttggcgttg ctctcgagcc
     3361 aatcgaagaa cccggcgtcc gcaatgatgc cgtacttgag cacctccgcg agcccggcac
     3421 gaagttcccg gtctcccagc gagttcagaa ccgtggtgtc cgcgatgacg cagcgtggct
     3481 ggtagaacgc cccgatcatg ttctttccgc gcggatggtt gatcgccgtc ttgccgccca
     3541 ccgacgagtc cacctgggca agcagcgtgg tcggcacctg aacgaaggcc accccgcgct
     3601 gaaacgtggc ggcaacgaaa cccgtgatgt ccccgaccac cccgccgccg agggccacca
     3661 gcgtggttga gcggttgtgc cgccgctcca tgagaaaatc catgatggcg ccgtagcttt
     3721 ccaggttctt gtgctgctcg ccatcgggca gggtgtagac gtcggcatcg attcccgcga
     3781 gggcttgggc cacgcgaggc aggtacaagg gccccaccac atcgttggtg atcacggcga
     3841 cctggccgcc gtgcacctga tcgcgcagca gctctccgcg tcccagcagg ccgtcaccaa
     3901 tgtgaatggg gtaactgcga tcccccaggt cgacctcgag cgtcacgtca gtcaaatcaa
     3961 accatcctcg cgcagcttgc gctcgatcgt gcgggccagc accttcggac cctgccggtc
     4021 ggttacgaat gtgtaatcgg ctatttccgc gtagagcgga gccctctcgc gctgcaggcg
     4081 agcgagggtc tcgcgggcgt caccgccctg cagcagcggc ctcttgttgt cgcgcgccgt
     4141 gcgctccacg agccgctcga gggggctgtc gaggtgcac
//
LOCUS       DBNHLM010000121        13598 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_914773, whole genome shotgun sequence.
ACCESSION   DBNHLM010000121 DBNHLM010000000
VERSION     DBNHLM010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13598
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_914773"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="AC16QF_05630"
     CDS             complement(<1..783)
                     /locus_tag="AC16QF_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR6448067.1"
                     /translation="MSTEQDTVVIRTIVSQDLLDVLVRVGLIGILLVMSFRIFSPFLS
                     ILLWALVLAVSLYPVHLKLVGKMGGRQGASATVIVIVGMLLIGTPTIALGSLFAGYIH
                     ETYQAFATNSVTVNPPRPEVAEWPIIGERMYAAWSAAASDMPAFLEELQPQLGVLTDR
                     ALSTAASTMGDVLLFLGALIIAGIMMAFGQSGSDAMGRILSRLSGPENGPRLHNLTTA
                     TIRSVATGVIGVAFIQALLLGVGFAFAGIPAAGLLALVVMLVG"
     gene            complement(850..3027)
                     /gene="selD"
                     /locus_tag="AC16QF_05635"
     CDS             complement(850..3027)
                     /gene="selD"
                     /locus_tag="AC16QF_05635"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR6448068.1"
                     /translation="MQLSAPPVRDLVLVGGGHSHVQVLKSFGMRPVPGTRLTLVSREV
                     YTPYSGMLPGYVAGFYDWHDIHIDLGALASFAGARLIVDEVTGADLDGGRLRLAAHPD
                     LHYDLLSINVGAVPRGVGQGVPVKPIGRFLPQLERVREEAGPGTSIAVVGGGAGGVEL
                     ALAMSRTMPHGVEVKLVTDELLPGYPAGVVRRLEKAVLRSGVVLIRDFRALDCRHGLL
                     ESSDGRSTAADHAFWVTGVMAASWPAAAGLATDVSGFIEVDRCLRSTSHESVYAAGDV
                     ACLIDQPRPKSGVYAVREGPALTRNLRRAIVGRPGIPFRAQKRFLSLIGTADGSAVAA
                     RGPWAASGRWAWRWKDWIDRRFVARFNRLPVMTEPGWSLPESLRDEAPDAMRCGGCGA
                     KLGASPLMRVLERLPPQVSGNVRVGIGDDAAILGATGGETILTVDGFRSLVSDPYLFG
                     RITAHHALNDIIAMGGKGTAALAMASVPLMSEALMEEDLFQLLRGAVDVLNAHEVPLV
                     GGHSSEGAELSLGLTVAGVPDGPTLTKGGAEPGDALVLTKPLGTGSILAGQMRGAVRS
                     DSVSAAIASMDASNASALVVFRRYGIHAATDVSGFGLLGHLGEMLRASALGVRLDLNA
                     VPFIEGAVEAVSAGAVSSLQDNNELALADFALEGCEPGDATVRLLVDPQTSGGLLGCV
                     PEAEAEACVRDLREVGYPRAAVIGRVLSNGWTVTAEREARPLS"
     gene            complement(3063..3380)
                     /locus_tag="AC16QF_05640"
     CDS             complement(3063..3380)
                     /locus_tag="AC16QF_05640"
                     /inference="COORDINATES: protein motif:HMM:NF046101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA3496 family putative envelope integrity
                     protein"
                     /protein_id="HIR6448069.1"
                     /translation="MSQVNDEDAGALEEREEFSDQTEFVDEDVSEDDAEIERGDGEFG
                     DDEALGDDLDDYDDDVGAVDDELTLKEQNARSLAIRRAIEQRLERKRLDEDLDYLDFD
                     FDE"
     gene            complement(3753..4733)
                     /locus_tag="AC16QF_05645"
     CDS             complement(3753..4733)
                     /locus_tag="AC16QF_05645"
                     /inference="COORDINATES: protein motif:HMM:NF015558.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HIR6448070.1"
                     /translation="MQRPADAAPAEDSPGSAPMVGSALGIGIACAALPPLIYFGNPAF
                     ALLVGGAIVLVIDRGLFPQASALSRYCLQTAIVLLGFRLSLDTLWSLSADYTWTVAAY
                     VLATLGLGMLIGRALRVEAASSKLITAGTAICGGTAIATLSTLVRAQPQQTAVALAIV
                     FLLNVVALFTFPTIGQWLDLSQLEFGLWSALAIHDTSSVVATAAIYGDEAAEVATTIK
                     LGRTLWIIPVALGASLMQTGESARLRVPGFILLFIVAAAVGTFLELPAAGPKAAGALS
                     KALLVVALFLVGTELTRATVRRIRGRVLWQALALWGLVVPGSLLVILQFT"
     gene            complement(4717..5523)
                     /locus_tag="AC16QF_05650"
     CDS             complement(4717..5523)
                     /locus_tag="AC16QF_05650"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6448071.1"
                     /translation="MVHPFFDPDSCSYSYAVVNPESRACAIIDAVLNYDLATGETSTA
                     GADQLVEFVKANGLIVEWILDTHVHADHLSAAAYLKSHFVCAQTAIGDQVVQVQGHFA
                     DLLGVAGAIDGSQFDRLLKDNDRICLGHACGRVMHTPGHTPACVTYVFGENAFVGDTL
                     LMPDYGTARCDFPGGDARLLYRSIQKIFALPDATRLFMCHDYAPNGRGYHYLTTVGEE
                     KRHNVHLGAGVCEEAFARMREQRDATLAPPKLMIPALQTNICGGRRAATG"
     gene            5661..6131
                     /locus_tag="AC16QF_05655"
     CDS             5661..6131
                     /locus_tag="AC16QF_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008367625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR6448072.1"
                     /translation="MPTYDRADRLILETLQNDASLSNAELAERIGLSANACWRRTRRL
                     EERGVIRKQVALLSQEKLNLKVTVFVGIRTNEHNESWLERFAEGVKAIPEVVEFYRMS
                     GDTDYLLKIVAEDIADYDRVYKHLISVANLHDVSSSFAMERIKSSTALPLTHLP"
     gene            complement(6164..6781)
                     /locus_tag="AC16QF_05660"
     CDS             complement(6164..6781)
                     /locus_tag="AC16QF_05660"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HIR6448073.1"
                     /translation="MADLAVVANVVLWACALALAVLVLALTRQVRSLHGRIAPAGALS
                     VNQALKVGMRAPSFNLETLGGGEVAIAAPGARQLLFFLAPDCPISRSMLPVLRSVHRA
                     EGGVALVLASDGDDPGEHLSFVAEMQLGEFPYVVSEELGRAYGVSKVPYAVLIDSDAR
                     IGALGIVNSREHLESLFEASERGVGSIQDYLAREGGGQPRYFEVR"
     gene            complement(6774..7295)
                     /locus_tag="AC16QF_05665"
     CDS             complement(6774..7295)
                     /locus_tag="AC16QF_05665"
                     /inference="COORDINATES: protein motif:HMM:NF018942.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HIR6448074.1"
                     /translation="MWEWVVHKVAAGTIACVFALALYHKGRDFPRFRASFEAYGIVPS
                     FLVPVCAPLLMGLEAGALVCLFLPDGPGSLLAFSVLGIYSVAIAVNLIRGRDYIDCGC
                     GDLPTPLSGWLILRNGLLMALAWPYGAAPEVAAVEPVTWLLVVMTVVVLMSLYLILEQ
                     LLANAGVGVANRG"
     gene            complement(8041..9207)
                     /gene="solA"
                     /locus_tag="AC16QF_05670"
     CDS             complement(8041..9207)
                     /gene="solA"
                     /locus_tag="AC16QF_05670"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HIR6448075.1"
                     /translation="MSYDVIVLGVGAMGAAALERLASRGARVLGIEQWSVPNSLGSSG
                     GDTRLIRKAYFEHPDYVPLLERAYEGWRRLEALSGLELLYPTGTVYVGPPDGPLIGGS
                     RMAAESHRLEFEPLAPAELHDRFPGFRLPDGCVSAFEPQAGFVLAGHGINAFAKLAIA
                     NGATLMSQTRVDGWRAESGGVVVSTSAGELEAGALVIAAGSWSSDICRLPRVALNVTR
                     QTLFWIWPERCAEFSLGRFPCWALEIAGHPGIYYGFPACVPNTARVGAKFAHHAPGET
                     CEPDGPKAPPSEAEYQATLRAVRSLLPGLRGSRIGSSICMYTNSRDGHFIVDRHPDHD
                     AVTVACGFSGHGFKFAPVMGELLADYALEGTSRLPAEFLRIGTRDLWHADEPKP"
     gene            9326..10399
                     /locus_tag="AC16QF_05675"
     CDS             9326..10399
                     /locus_tag="AC16QF_05675"
                     /inference="COORDINATES: protein motif:HMM:NF016214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR6448076.1"
                     /translation="MDPVSQAALGAVVARSVSHRQLGAGAVALGAAAGALPDIDVFFS
                     LDGDYFDQLVSHRGITHSLFFAPVVGPLFGWLAWRWTARRRNDPGEPAALRAWMLAMT
                     LAMLSHPLLDLLTPYGTQLLQPFSDARFAVAAMPIIDPIYTIVLLAGLVAGYRFTAAG
                     GRIAAATLLVSCAYLGYGWHLNEGAERFARAQLAAQGLPNVEVAAFPTILQIHHRRVV
                     ARTPDADRVGFLSMWDPCDIGWQREPRLPRSQVEAFLRTREGAIFDWFTMGWSHYAIQ
                     DGHMTATDLRYGSDENPRASVFTASVALDHLGRPSGNIAGGRFTPDANGRNLERLIAM
                     TYAPGCGTSPGRMAQTSLEAGAG"
     gene            10449..11972
                     /gene="nhaC"
                     /locus_tag="AC16QF_05680"
     CDS             10449..11972
                     /gene="nhaC"
                     /locus_tag="AC16QF_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HIR6448077.1"
                     /translation="MPSGPMDDDGLTMEQEAGRPNDPSNLDVVVAIVPTTVLLALSVA
                     LFGSDSSSGANQIALVIGAALATMVGVKNGHSWKVIEENIVGGISTAMPAIMILLSVG
                     SLIGVWMLSGTVPTMIYYGLLLLEPAVFYAASCLLCAVTALSIGSSWTVAGTLGIGLI
                     GVAAGLELSLPITAGAIISGAYFGDKLSPLSDTTNLAPAVTEIDIFTHIRHMTWTTLP
                     SFVFALGAFTAIGLMESTPETQTSLRVTLETLDEAFAINPLMLLPVALVVLLAGRKKP
                     AVACILAGVALGILMALVYQRPAIVRFADDASLGPTVAALKAIWIVLFDGYAGSTGEA
                     NLDELLSRGGMSSMLNTVWLILAAMTFGAAMEQAGVLRRLVHALASAARSTGNLIATT
                     LVTCIGVNVLAADQYMSIVLTGRMFRSEYRRRGLHEKNLARTLEDGGTITSPLVPWNT
                     CGAYMAATLGVPTLAYLPFCFFNLANPFVSAAYGYAGFKIEPLKPGGEGEAAPSRAQ"
     gene            complement(11997..13187)
                     /locus_tag="AC16QF_05685"
     CDS             complement(11997..13187)
                     /locus_tag="AC16QF_05685"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR6448078.1"
                     /translation="MSPLLPSAGGGLKLFSIFGIEIRLDYSVAIIFGLVVYSLGGGLF
                     PQWHPDWPPSTAWLTALVAGVLFFASLLAHELSHSLTARRYGIRVPRITLFLFGGVAE
                     IESEAQTPSAELTIAIAGPLMSALLGAVFTVVAFGSMDTLAREAFAEDPETVIASLSP
                     GYTACLWLGSVNMMLAVFNLIPGFPLDGGRVFRAIIWKLTGNQLRATRLASTTGRWFG
                     WAIMGIGLWNLLVLKSAGGLWLLLIGWFLSHLAAASYSQALAQQTLAPLRVRDVMRTR
                     FEFAPSGITIDEFINDYLLRSNQILWPLQGPAGVVGAVNFEQVASVPPDQRATRLVQE
                     VMVPVDRLPALDADDLATNALNTLLGAGDQPVPVVSGQRIVGLVRGSDILKWVMLHQG
                     GTPS"
     gene            complement(13184..>13598)
                     /locus_tag="AC16QF_05690"
     CDS             complement(13184..>13598)
                     /locus_tag="AC16QF_05690"
                     /inference="COORDINATES: protein motif:HMM:NF021511.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HIR6448079.1"
                     /translation="YDRFVHLMYGLLIVYPVRELFLRVADARGFWGYFFPLLVVMSSS
                     LAYELLEWGAAFVFGGELGMAYLGTQGDVWDAHKDSLLATGGALAATLVIAAVHSSFD
                     RDFGRDWSQSLTVKNPDPLGEAAIGRYLNERENAP"
BASE COUNT         2641 a         4545 c         4142 g         2270 t
ORIGIN      
        1 gccgaccagc atcaccacca gcgccagcaa acctgcggct gggatgcccg caaaggcgaa
       61 tcccacgccg aggagcaggg cctgaatgaa cgccacgccg atcacgcccg tggccaccga
      121 gcgaatcgtc gccgtcgtca ggttgtgcag gcggggcccg ttttccgggc cggacagccg
      181 actgaggatc cgacccatgg cgtcgctgcc cgactggccg aacgccatca tgatgcccgc
      241 tatgatgagg gcaccgagaa agagcagcac atcgcccatg gtgctggccg ccgtgctcag
      301 cgcacgatcc gtcagaacgc cgagctgggg ctgcaattcc tccaggaagg caggcatgtc
      361 gcttgccgcc gccgaccagg ccgcatacat ccgctccccg atgatgggcc actcggcgac
      421 ctccggcctc ggtgggttga cggtgaccga gttggtcgca aacgcctgat aggtttcgtg
      481 gatatagccg gcaaaaaggc tgcccagggc tatggtcggc gtgccgatca gcagcatccc
      541 gacaatgacg atcacggttg ccgacgcgcc ctgccgacca cccatcttgc cgacaagttt
      601 cagatgcacc gggtacagcg acacagcgag aacgagcgcc cacaggagaa tgctgagaaa
      661 cggagaaaat atccggaagc tcatcaccag aaggatgccg atcagcccca cccggaccag
      721 cacgtccagc aagtcctggg aaacgatggt gcggatgacg acggtgtcct gctcggtgct
      781 catttccact cccccaactg atgccaaacg gcgaatcata cgcgaggacg cgcatggtcg
      841 tctcgcggct cagctgagcg ggcgcgcctc tcgttcggcg gtaacggtcc agccgttcga
      901 cagcacccgg cctatgacgg ccgcacgcgg gtaaccgacc tcgcgcagat cccgaacgca
      961 ggcttcggcc tccgcctccg gcacgcaccc gagcagcccg ccggaagtct gagggtcgac
     1021 gagcagacgc acagtcgcat cgcccggctc gcagccttcc agcgcgaaat ccgcgagggc
     1081 gagctcgttg ttgtcttgca gcgagctgac ggcaccggcg ctgacggcct cgaccgcacc
     1141 ctcgatgaaa ggcaccgcgt tgaggtcgag ccgcacaccc agggcggacg ccctgagcat
     1201 ctcccctagg tgaccgagca acccgaagcc cgacacgtcg gtggcggcat ggatgccgta
     1261 gcgacggaac acgaccagag ccgatgcgtt cgatgcgtcc atgctggcaa tagcggcgga
     1321 gacgctgtcc gagcgaacgg cgccgcgcat ctgtcctgcc aggatcgaac ccgtgccgag
     1381 cggcttggtg agcaccagcg cgtcccccgg ttccgccccg cccttggtca aggtcggtcc
     1441 gtcgggaacg cccgcaacgg taagacccag gctcagctcg gctccctcac tcgagtgccc
     1501 gcccaccagg ggcacctcgt gcgcattgag cacgtccacc gcgccgcgaa gcagctgaaa
     1561 gagatcttcc tccatgagcg cttcggacat cagaggcacg ctcgccatcg ctagggcagc
     1621 cgtaccctta ccgcccatgg cgatgatgtc gttcaaggcg tggtgggccg ttatgcggcc
     1681 aaacaggtaa gggtcggaga cgaggctgcg gaagccgtcg acggtgagta tcgtctcgcc
     1741 accggtcgcg ccgaggatgg ccgcgtcatc gccgatgccg acccgcacgt tcccggacac
     1801 ctgaggcggc aggcgctcga gcacgcgcat cagcgggctc gcgccgagct tcgccccgca
     1861 gcctccgcat cgcatggcgt ccggcgcttc gtcacggaga ctctctggca gcgaccagcc
     1921 cggttccgtc atgaccggca gccggttgaa gcgcgccacg aaccggcggt cgatccaatc
     1981 cttccatcgc caggcccagc ggccgctggc cgcccaaggg ccgcgagcgg cgacagcgga
     2041 cccgtcggca gttccgatga ggctcaagaa gcgcttctgt gcccggaacg gaatcccggg
     2101 ccgtccgacg attgcccgcc ttagattgcg ggtcagcgcc gggccctccc gaacggcgta
     2161 cacgcccgac ttgggcctcg gctgatcgat tagacaggcc acgtccccgg ccgcgtaaac
     2221 gctctcatga ctcgtggacc gcaggcagcg atcaacctcg atgaacccgc tgacatccgt
     2281 ggccagccca gccgcagccg gccacgacgc tgccatcacc ccggtaaccc agaacgcatg
     2341 gtccgccgcc gtcgaccgcc catcggagct ttcgagcagc ccgtgcctgc aatccagcgc
     2401 ccgaaagtct cggatgagca cgacccctga ccggagcacc gccttctcca gccgtcggac
     2461 cacgccggcc gggtagcccg gcagcagctc gtcggtcacc agcttcacct cgaccccatg
     2521 cggcatcgtg cggctcattg ccagcgcaag ctccacgccg cccgcccctc cgccaacgac
     2581 ggcgatagac gtaccgggcc cggcttcttc tcgaacccgc tcgagctggg gcagaaaccg
     2641 gccgatgggc tttaccggta ccccttgacc caccccccgg gggactgccc ccacatttat
     2701 ggagagcagg tcgtaatgca gatcgggatg agcggcgagg cgcagccggc ccccgtcgag
     2761 atcggctcct gtcacttcat cgacgatcag tcgcgctccg gcaaacgacg caagggcgcc
     2821 tagatcgatg tggatgtcgt gccagtcgta gaacccggcc acatagccgg ggagcattcc
     2881 ggagtaaggc gtgtagacct cacgactgac caaggtgagc cgcgtccccg ggacggggcg
     2941 cataccgaaa gacttgagta cctggacgtg gctgtggcca ccaccgacca gcaccagatc
     3001 cctgacgggc ggagcactga gctgcatcgg gaaagtatac gggctaagac ccttttatgg
     3061 cctcattcat cgaaatcgaa gtcgaggtaa tcgaggtctt cgtccagacg cttccgctcc
     3121 aggcgctgct cgatggctct acggatggca agcgatctgg cgttctgttc cttcagggtg
     3181 agctcgtcgt caaccgctcc gacatcgtcg tcgtagtcgt cgagatcgtc gccaagtgct
     3241 tcgtcgtcac caaactcgcc gtcaccccgc tcgatttcag cgtcgtcttc cgagacgtcc
     3301 tcgtcgacga attccgtctg atcgctaaac tcttctcgtt cctcgagagc gcctgcgtct
     3361 tcgtcgttga cctgggacat ggtggaacga cccatcccga aaagccttgc tcgattccgc
     3421 gctttttagg cccgcgtcct acgcgtgtca agggattttg aggacgaatt accgacggcc
     3481 cgatgggagt ttccattggg cgtcggagac ctcgatgctg accggccgcg ccggcggata
     3541 ccaagcgccg atcaggcgaa caggggggtc agcagataag ccacgaacgc ggcgactgcg
     3601 aggagactca ggttgtcttt gataccgatc ttagccatga gccaaggtta ggcattcgca
     3661 acgatcaggt gaagaattga ttgcttctat ggatattcca tcaggtgctt aaagcgatgg
     3721 ccttcgccgc catcgcgggc ggctcgaacc cgtcaggtga attgaagaat gaccagcagc
     3781 gagccgggca caaccaaccc ccacaatgcc agcgcctgcc acaggacccg tcccctgatc
     3841 cggcgcacgg tggcccgggt gagctcggtg ccgaccagaa atagcgcgac caccagcagc
     3901 gctttgctca aagcgcccgc cgccttgggg cccgctgccg gcagctcgag gaacgtgcca
     3961 accgccgcgg caacgatgaa caggaggatg aacccgggaa cccgcaaccg cgcgctctcc
     4021 ccggtctgca tcaggctcgc ccccagcgcg accggaatga tccagagggt tcttccaagc
     4081 tttatcgtgg tggctacctc cgcagcttcg tcaccgtaga tggcggcggt ggcaaccacc
     4141 gagctcgtat cgtgaatggc gagcgcagac cagaggccga attccagctg gctgagatcg
     4201 agccattgac cgatcgtggg aaaggtaaaa agagcaacca cattgagcag aaacacgatg
     4261 gcaagggcca ccgccgtttg ctgaggctgt gcccgcacaa gtgtgctcag ggtggcgatc
     4321 gccgtgccac cgcagatggc ggtgcccgcc gtaatcagct tgctggacgc tgcctcgaca
     4381 cgcagcgcgc gaccgatcag catgcccagg ccaagggtcg ccagcacgta ggcagccacc
     4441 gtccaggtgt aatccgcact caggctccac aaagtatcca ggctcagccg aaaccccagc
     4501 agaacgatgg ccgtctgcag gcagtagcga cttagcgccg acgcctgagg aaacaggccg
     4561 cggtcgatta ccaggacgat ggcgccaccc acgagcaggg cgaaggcggg attgccgaaa
     4621 tagatcaggg gcgggagagc tgcacacgcg atgccgatac ccagagcgct ccccaccatc
     4681 ggggcgctgc cgggtgagtc ctctgcagga gcggcgtcag ccggtcgctg cacgcctgcc
     4741 tccgcagata ttcgtctgca gcgcaggaat catcagcttc gggggcgcaa gggtggcgtc
     4801 ccgctgttcg cgcatacgtg caaaggcctc ctcacagacc ccagcgccaa ggtgaacgtt
     4861 gtggcgtttc tcctcgccca cggtggtcag atagtgatac ccgcgcccat tcggggcgta
     4921 gtcgtggcac atgaaaagac gggtcgcatc tggcagagcg aagatcttct gaatggatcg
     4981 gtacagcaat cgtgcgtccc cccccggaaa gtcacatctg gccgtgccgt agtctggcat
     5041 gagcagcgtg tcccccacaa aggcattttc gccgaacacg taggtaacac acgctggcgt
     5101 atgtcccgga gtgtgcatca cccgcccgca ggcatggccc aggcagatgc ggtcgttgtc
     5161 ctttaggagt cggtcgaact ggctgccatc gatcgcgccg gccacgccga gcagatcggc
     5221 aaagtggccc tgcacctgca ccacttgatc cccgatggcg gtctgggcgc acacgaaatg
     5281 gctcttcagg tacgcggctg cagacagatg gtcggcgtgc acgtgggtgt cgagaatcca
     5341 ctcgacgatc aacccgttgg ctttgacgaa ctccaccagc tggtctgcgc ccgccgtgct
     5401 ggtctcaccg gttgcaaggt cgtagttcag caccgcgtca atgatcgcgc acgcgcggct
     5461 ctcaggattc accaccgcat agctgtagga acatgaatca ggatcgaaaa acgggtgcac
     5521 cattaggctc acaagcttct cctgcggatg acttgagaag agtttattgc agatggagcg
     5581 gtaaaaagtc ccgttatgtt tcacatattc ctggttatac gtaatattat tctacgattc
     5641 ttactaattt cagacgaatt atgcctactt acgatcgcgc tgaccgactt attctggaga
     5701 ccctgcagaa cgacgcttct ctttccaatg ccgagctcgc cgagcgcatc gggctgtccg
     5761 cgaacgcctg ctggcgccgc acccgccgcc tggaggaacg gggggtcatt cgcaagcagg
     5821 tcgcgctctt gagccaggag aagctgaacc tcaaggttac cgttttcgtg ggcatccgaa
     5881 cgaacgagca caacgagagc tggctggagc gattcgcaga aggggtcaag gccatcccgg
     5941 aggtcgtgga gttttaccgc atgagcgggg ataccgacta cctgctgaag atcgtggccg
     6001 aggacatcgc cgattacgac agggtctaca agcatctgat ttcagtcgca aacctgcacg
     6061 acgtgtcatc ctccttcgcc atggagcgga tcaagtcctc cacggccctg ccgctgacgc
     6121 accttccatg aggatgtcct gacctagcgg ggcggcacga gcattaacga acctcgaaat
     6181 agcgcggctg gcctccccct tcccgggcca gatagtcctg aatggacccg accccgcgct
     6241 cgctggcctc aaacaggctc tcaagatgct ccctggagtt gacgatcccc agcgcgccga
     6301 tccgagcgtc ggaatcaatc agcacggcat agggcacctt gcttacacca taggctcgac
     6361 cgagctcctc ggagaccacg tagggaaact cgccgagctg catttcggcg acaaaagaca
     6421 ggtgttcccc cgggtcatct ccatcgctgg caagcaccag ggcgacgccg ccttcggcac
     6481 ggtgcaccga ccggagcacg ggcagcatcg accggctgat gggacagtca ggggcaagga
     6541 agaagagcag ttgacgcgcc ccgggcgcgg cgatcgccac ctctccccca ccgagggttt
     6601 ccaggttgaa cgacggggcc cgcataccaa ccttgagcgc ctgattcacg ctgagcgccc
     6661 cggccggcgc gattcgcccg tgtagcgaac gcacctgccg agtcagcgcg agcacgagga
     6721 cggcaagggc aagagcgcaa gcccagagca cgacgttcgc gacaacggca aggtcagcca
     6781 cggttcgcga cgcccacgcc cgcgttggcg agcagctgct ccaggatcag atacaggctc
     6841 atcagcacga ccacggtcat cacgaccagc agccaggtga cgggctcgac agccgcgact
     6901 tccggtgccg ctccgtaagg ccacgcaagg gccatcagca ggccgttgcg aagaatcagc
     6961 cacccgctca ggggagtggg gagatcaccg catccgcaat cgatgtaatc cctgccgcgt
     7021 atgaggttca cggctatagc cacgctgtag attccgagca ccgagaatgc aagcagcgag
     7081 ccgggaccgt ccggcagaaa gaggcagacg agggcgccag cctctagccc cattagaagc
     7141 ggggcgcaga ccggaacaag gaaactcggg acaattccgt aggcctcaaa agacgctcga
     7201 aaccgcggaa agtcccgccc cttgtgatac agcgcgagcg caaacacgca ggctatggtg
     7261 cccgcagcca ccttgtgaac gacccattcc cacattgcag tcgcgcccgg cttcccctct
     7321 gactcaccgc tctctccgga ccgtaaagcc gtccacgtcc ggaaaccctt cgccacggag
     7381 gttagcattg catgtgggcg cgcgtacaat ggcgcgcttt ccagctgagg cggcattgtg
     7441 agcgggcaga gcccgcccct gttcagtgaa gcgacacgtc gctacgttct cggcatccca
     7501 attgcccgct gggcatacct ttcggcatcc tctccggttt tctggcgggc ggctggatga
     7561 aggagttctt cggctgacgg gtcgcattct tcgtcgtcgg gataccccgc atcgtagcgg
     7621 ctgtggttgt tcggctgacc gtccgcgaac cgcccagcgg tatggccgag ggacgcgaac
     7681 cggccatcga gcagcccacc atcatggcga cgtgccgaac gctgtggcga agaagggcat
     7741 tccgggcggt atcggaatcg ccctcggagg ccatctggcg gaccggctcg gcgcccgcga
     7801 tcggtggcat aagaaatact gatttttgtt cttaatatca ttaggttagg cctataacct
     7861 aaaaccgtag gcattatcag cgatctcctc caacctgcct acgggccgga gtcgctccgc
     7921 tacagccttc tcgtctgctc gttcgtgaat gtctgggctg ccgcccacta ttacccggcg
     7981 ggaacatacc tcccaggcga cctggcggcc gcagagcgcg gcagcgactg accaccgagc
     8041 ctatggtttc ggttcatcgg catgccacag atcgcgggtc ccgatccgaa ggaattccgc
     8101 cggcagccgc gaggtaccct cgagcgcgta gtcggccagc agctcgccca tcaccggcgc
     8161 gaatttgaat ccgtggccgc tgaacccgca agccacggtg accgcgtcat gatcggggtg
     8221 ccgatcgacg atgaaatgac catcgcggga attcgtgtac atgcagatcg acgagccgat
     8281 gcgcgagcct cgcagcccgg ggaggagaga cctcacggcg cgcagcgtcg cctgatactc
     8341 ggcctcggag ggcggcgctt tcgggccatc gggttcgcat gtctcgccgg gagcatgatg
     8401 ggcgaacttg gctcccacac gcgcggtatt cgggacgcag gcagggaacc cgtagtagat
     8461 ccccggatgc cccgcgatct ccagcgccca gcacggaaac ctgcccagag agaattctgc
     8521 gcagcgctcg ggccagatcc agaaaagcgt ttggcgggtc acgttcagag cgacgcgggg
     8581 cagccgacag atgtcgctcg accagcttcc cgccgcgatg acgagtgcac cggcctcgag
     8641 ctccccagcg ctggtgctga ccactacccc gcctgattcg gcccgccaac cgtctacccg
     8701 ggtctggctc atcagcgtag cgccgttggc aatggcgagc ttcgcaaacg cgttgatgcc
     8761 gtgaccggcg agcacgaacc cggcttgcgg ctcgaaggcc gagacgcagc cgtcgggtaa
     8821 gcgaaacccc gggaaccgat cgtgcagctc ggctggcgcg agcggctcga attccagtcg
     8881 atgggattcc gcagccattc gtgagccccc gatcagcggc ccatccggag gccctacata
     8941 gacggtgccc gtcggataga gcagttcgag cccgctgaga gcctcgagtc gacgccagcc
     9001 ttcgtaagcc cgctcgagca gcggaacata gtcgggatgc tcgaagtaag cctttcggat
     9061 gaggcgcgta tcacccccac tggagcccaa ggagttgggc accgaccatt gctcgatgcc
     9121 cagcaccctg gcgccccggg atgccagccg ctccaaagcc gcggcgccca tggcgccgac
     9181 accgaggacg atcacgtcgt agctcatggc gcaatcctat ctcagctcgg cgaagcgggc
     9241 agagggaaca gtgcacattg cggctgccaa tcggctccat cgccggtatg atgctcgcaa
     9301 ctgctccagc cgtcgtgtca cttccgtgga tcctgtctcc caagccgccc tcggcgccgt
     9361 ggtcgcccgc agtgtcagcc atcgccaact tggtgccggc gccgtcgcgc ttggggccgc
     9421 ggcaggcgcc ctgccggaca tcgacgtgtt cttctccctg gatggtgact acttcgacca
     9481 gctggttagc catcgcggga tcacgcattc gctgtttttc gcccccgtgg tgggccccct
     9541 cttcgggtgg ctggcctggc gctggaccgc ccgccgccga aacgaccccg gggagccggc
     9601 ggcattgcgc gcctggatgc tcgcgatgac cctggcgatg ctttcccacc cgcttctaga
     9661 tttactaacg ccgtacggca cgcaactcct ccagcccttc agcgacgcga gattcgcggt
     9721 ggcggccatg cccatcatcg atccgatcta caccatcgtg ttgctggctg ggctcgttgc
     9781 cggatatcgg ttcacggcgg ccggggggag gatcgcggca gcaacgctgc tcgtctcctg
     9841 cgcttacctg ggttacggct ggcacctcaa cgagggcgcg gagcggtttg cccgcgccca
     9901 gctcgcagcc caggggttgc cgaacgtcga ggtagccgcg ttcccgacga tcctgcagat
     9961 tcaccaccga cgtgtggtcg cacgcacccc ggacgccgac cgggtcgggt tcctctccat
    10021 gtgggatccg tgcgatatcg gctggcaaag ggaacctcgc ctgccccgat cacaggtgga
    10081 ggccttcctc cgcacgcggg aaggggcgat attcgactgg ttcaccatgg gttggtcaca
    10141 ctacgccatc caggacgggc acatgacggc aaccgatctt cgctacgggt ctgatgagaa
    10201 cccccgggca agcgttttca cggcatcggt cgccctggac cacctcgggc gcccctcagg
    10261 aaatatcgcc ggcggccgct tcaccccgga cgccaacggc cggaacctcg aacggctgat
    10321 cgccatgacc tacgcacccg gctgcggcac gagccctgga cggatggccc aaacgagcct
    10381 ggaagccggc gccggctgat ggcgtcaacg gagcatctgc aacgatcgct tcgaacgcag
    10441 tcagggccat gccttccgga cccatggatg acgacggtct gaccatggag caggaagccg
    10501 gccggccgaa cgacccaagc aatctggacg tcgtggtggc catcgtcccg acgacggtcc
    10561 tcctggcgct gtcggtcgcc ctcttcggca gtgactcatc gagtggcgcc aatcagattg
    10621 ccctggtcat cggagctgcg ctggccacca tggtcggcgt gaagaacggc cattcctgga
    10681 aggtgatcga agagaacatc gttggcggca tttccaccgc gatgccggca atcatgattc
    10741 tcctgtcggt cggcagcctc atcggggtgt ggatgctgtc aggaaccgtg cccacgatga
    10801 tctattacgg gttgctgctt ctcgaaccag cggtgtttta cgccgcgagt tgtctcctgt
    10861 gcgccgtgac ggcgctctcc atcggaagct cctggaccgt agcgggcacg ctcggcatcg
    10921 gtctgatcgg ggtagccgcc gggctcgaac tttcgctgcc gatcaccgct ggggccatca
    10981 tctccggagc ctatttcggc gataagctct ccccactttc cgataccacg aatctcgccc
    11041 cggccgtgac cgagatcgac atcttcaccc acatacggca catgacctgg accaccctgc
    11101 cgagcttcgt gtttgccctt ggggcgttca ccgccatcgg cctgatggaa agcacgcccg
    11161 agacgcaaac gtcactgcgc gtcaccctgg aaaccctcga cgaagctttt gccatcaacc
    11221 ccctgatgct gctccccgtc gccctcgtcg ttcttctcgc gggcagaaag aaaccggccg
    11281 ttgcctgcat ccttgccggc gtggctctgg gaatcctgat ggcgctcgtc taccaacgtc
    11341 ccgccatcgt ccggtttgca gacgacgcgt ccctggggcc cacggtcgcc gcgctcaagg
    11401 caatctggat cgtcctgttc gacggctacg ccggctccac cggcgaagcc aatcttgacg
    11461 agttgctctc tcgcggcggg atgagcagca tgctcaacac cgtctggctg atactggcag
    11521 caatgacctt cggagccgcc atggaacagg ccggggtgct tcgccggctg gtccatgctc
    11581 tggcatccgc agcgcgcagc acgggcaatc tcatcgcgac gacactggtc acctgcatcg
    11641 gggtgaacgt gctggccgcc gatcagtaca tgtccatcgt cttgaccggc cgcatgttca
    11701 gatcggaata tcgacgccgc gggctgcacg agaaaaacct tgcccggacc ctcgaagacg
    11761 gcggcaccat cacctcgcct ctcgtcccat ggaacacctg cggcgcctac atggcagcga
    11821 cgctgggcgt acctaccctg gcctatctgc cgttctgttt cttcaatctg gccaacccgt
    11881 tcgtctcggc cgcctacggc tacgcggggt tcaagatcga gccgctaaaa cccggcgggg
    11941 aaggcgaagc tgccccctcg cgcgctcaat gacgaagacc gaagacggga acgtgatcac
    12001 gacggagtgc caccctggtg aagcatcacc catttcagga tgtccgagcc gcgcaccaga
    12061 ccgacgatcc gctgaccgct cactaccgga accggttgat ctccggcacc gagcagcgtg
    12121 ttcagggcat tggtcgccag atcgtcggca tcgagcgcag gcagcctgtc caccggcacc
    12181 atcacctcct ggaccagcct ggttgcgcgc tgatccggag gcacgctggc aacctgctcg
    12241 aagttgacgg ctccaaccac tcccgcgggg ccctgaagcg gccagagaat ctggttgctc
    12301 ctgagcaggt agtcgttgat gaactcatcg atcgtgatgc ccgacggtgc gaactcgaag
    12361 cgagtgcgca tcacgtcgcg gacccgaagg ggagcgagcg tctgctgagc gagggcctgg
    12421 gagtaactgg ccgctgcgag atggctcaaa aaccagccga tgagcagcag ccagagcccg
    12481 ccagcggatt tgagcaccag caggttccac aggccgatgc ccatgatggc ccagccgaac
    12541 cagcgaccgg tggtacttgc cagccgtgtt gccctgagct ggtttccggt cagcttccag
    12601 ataatggcac gaaacaccct gccgccatca agcgggaaac cgggaatcag gttgaacacg
    12661 gccagcatca tgttcacgga gccgagccac agacaggcgg tgtatcccgg gctcaggctg
    12721 gcgatgacgg tttccggatc ttccgcaaac gcttccctcg ccagggtgtc catgctccca
    12781 aacgcaacca ccgtgaacac cgcgcccagc agagcgctca tcaacggacc cgcaatggct
    12841 atggtcagct ctgctgacgg ggtttgcgcc tccgactcga tctcggctac gccgccaaac
    12901 aggaacaggg taattctcgg cacccttatc ccgtatcggc gggccgtgag cgaatgagaa
    12961 agctcgtggg ccagcaacga tgcgaagaag agcacgccgg ccaccagcgc cgtcagccac
    13021 gccgtcgagg gcggccagtc gggatgccac tggggaaaca gcccgcctcc caggctgtac
    13081 acgacgaggc cgaagatgat ggcaacgctg taatcgagcc ggatctcgat gccgaagatg
    13141 ctgaacagct tcagccctcc gcccgcactc ggcaacaatg gactcatggg gcattctccc
    13201 gttcgttcag ataacgcccg atggctgcct cgcccaaggg atcgggattc ttgacagtca
    13261 ggctctgcga ccaatcccgg ccgaagtccc ggtcgaagga tgagtggacg gcggcaatca
    13321 ccagggtggc ggccagcgcg ccgccggttg ccagaaggct gtccttgtgg gcgtcccaga
    13381 cgtcaccctg ggtgccgaga taggccatac ccaactcgcc gccgaacacg aaggcggccc
    13441 cccactccag cagctcgtag gccagcgagg acgacatgac caccagaagc gggaaaaaat
    13501 atccccagaa acctctggca tccgcgacgc gcaggaagag ctctcgtacg gggtaaacga
    13561 tgagcagccc gtacatcagg tggacaaagc gatcgtag
//
LOCUS       DBNHLM010000122         9701 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_256288, whole genome shotgun sequence.
ACCESSION   DBNHLM010000122 DBNHLM010000000
VERSION     DBNHLM010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9701
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_256288"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..938
                     /locus_tag="AC16QF_05695"
     CDS             96..938
                     /locus_tag="AC16QF_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR6448080.1"
                     /translation="MLEYSEARQISDSERVEYLEFALGLAAAAGSAILPFFRAGAGVE
                     NKRTDGGFDPVTEADHAAERVIREQILGRYPEHGIFGEEFGLDAGNGLTWVVDPIDGT
                     RAFMSGLLHWGVLLALFDGERPVLGVMHQPFTAEFFAGDCHNAHYHRGETSQALEVRE
                     CKGLGQAVLGTTSPKLFTSPFERRGLDAIEERVKLSRYGGDCYLYAMLAMGQLDIVMD
                     AGLQPYDVQALVPIIEGAGGVVTTAQGGDASMGGFVVATGSAELHAEVLATIRAGSEL
                     GPGS"
     gene            complement(1251..2090)
                     /gene="surE"
                     /locus_tag="AC16QF_05700"
     CDS             complement(1251..2090)
                     /gene="surE"
                     /locus_tag="AC16QF_05700"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014077.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR6448081.1"
                     /translation="MFAFAAQPCTDRPLNILLVNDDGFDTPGIIALHRELKASGHNVR
                     RVAPDRNYSGSSASLTIDLIAAPRIPDEEFTEVYAVSGSPATAVLVGATAIFPPEQPV
                     DLVVSGINEGANLGPATVISGTVGATIAAMKALARPIPGIAVSTNPIAEDEAAPENHR
                     HLKTVAGFTSRLVRRLFCASQPWSKGELALNVNYPPLEPEAVKGVRVAAQGKAPYYSM
                     GFKAAAPDRYAPDFGRAEPSQDIENADTFLFRQGYITIVPIDGDYTAGGRPLPASLED
                     LEP"
     gene            2213..2971
                     /locus_tag="AC16QF_05705"
     CDS             2213..2971
                     /locus_tag="AC16QF_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6448082.1"
                     /translation="MASLELMDANQLDYSPIRRAGVIGDVHAEDERLAGALEWLSGQD
                     VDVILCTGDVADGVGCVNTACRLLRDADVLTVAGNHDRWLLQDRVRHVADAHFLADLD
                     DESRDYLTALPRSRTLNTVAGPLLLCHGVVENDLAKVWPGTERSPIERSTEMDDLVAA
                     STHRFVVNGHMHYRVLIDFEGVVLVNAGTIRGPFSGVTVIDFEGDFAAAYERDGNGRA
                     HRVAEHELTRNGERRIWRDTQEFDGSWQPVTLHP"
     gene            complement(3000..3596)
                     /gene="purN"
                     /locus_tag="AC16QF_05710"
     CDS             complement(3000..3596)
                     /gene="purN"
                     /locus_tag="AC16QF_05710"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR6448083.1"
                     /translation="MRIAALASHGGSILQAVIDAIENGTLPAELVLVVSNNSRSQALA
                     RASRHGIATLHLSAHTHRDPDELDAAIVRSLEEANADWVLLAGYMKRLGPRMLGRFRN
                     RIINTHPALLPKFGGQGFYGQRVHEAVIEAGETESGATVHVVNEAYDSGPILAQVRVP
                     VEKNDTAEVLEERVKRAERKLIVTTLAELAARPAASGY"
     gene            complement(3596..4369)
                     /locus_tag="AC16QF_05715"
     CDS             complement(3596..4369)
                     /locus_tag="AC16QF_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448084.1"
                     /translation="MTTYEVVARNFSQAHENRIHSDEVAQRYGFTGALVPGVAVYGHL
                     THPVVERLGEDWLGRSVSSVRFLKPAYDGDRLHLDCESRGSALLVSCRNDAGTLLAEL
                     HTEQPRDMPEPVTAAVLDEPRRTAERAEISWEAVVENETFEPWHWQITEDGNAQYAAE
                     VADHLPIYRLCAHPHWLLSTANRALTREYAMPAWIHVGSDVRHRALVKVGDTVEVRAA
                     LMEKWRRKGHEFLRLYVAYRRGRELTTEIFHTAIFKVAA"
     gene            4540..5454
                     /locus_tag="AC16QF_05720"
     CDS             4540..5454
                     /locus_tag="AC16QF_05720"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014433.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HIR6448085.1"
                     /translation="MAALRGVRLVVVTGKGGVGKTSVAAGIASASARSGLRTVLVTLD
                     TGDTRHPLLDVPLEYQPYQAAPRLSVSRLDALEAVREYVRRNMPFSSLYENIFDGHAF
                     RNFASAAPGFEELMSLGKLYELCTSGEFDRVVFDAPATGHMKLLLDVPGATLRAVHVG
                     PLNHNAGKIQDLLLDGSRTRVVITTLAEEMAVEEAMDLERCCRNDHRMAVGPIVVNRL
                     VPERFSVEEIAALQSMEASTLELREAVAAVVAEHAEQRSQEEALRALSAAGSDVVSVP
                     RIVQACFDPAAFLEALEAHLMPLFGNAA"
     gene            5451..6566
                     /locus_tag="AC16QF_05725"
     CDS             5451..6566
                     /locus_tag="AC16QF_05725"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014433.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HIR6448086.1"
                     /translation="MSAALKLEGVVADRRIIICCGSGGVGKTTTGAALGVLAAQLGRS
                     ALVMTIDPAHRLAQAMGLQALGHEPAEIALEAPGRLSAMMLDTKHTFDRLVEQYAPSG
                     QVRDAIFANEYYRQLSSTLGGSRELVAMERVLEAATEEDYDLLIVDTPPAHHALDFLD
                     APRRLIDLIDGSLTNSLLKPYGMAARAQFNLFRQSSAVTLKFLERLTGFEMLADLSEF
                     LLAFSSMFDGFKERSHRVMELMRESGTTFLLICAPEEGSLEQVEQFRSRLEKDSLDIC
                     GVLVNRVHLMDPRAGDLDDSVLARIASLDVAEPRGAFLAERVAAAYEDRRTMARADAG
                     VMADRLGEDLPVQAVPHFHRDLHSMADLQAFAETVRN"
     gene            complement(6568..7917)
                     /locus_tag="AC16QF_05730"
     CDS             complement(6568..7917)
                     /locus_tag="AC16QF_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6448087.1"
                     /translation="MPANEILALLMFVSFIALVFTGFPVAWILGGLAVLFTAFAIVLD
                     VDFNLAIGVDWNYASITVDRIWNVMENWVMVALPMFIFMGLLLDRSGIANQLMTSFAR
                     LFGGLRGGLAITVTLIGLLLAATTGIIGASVVLLALLGMPAMLKQGYDRALAAGTVCA
                     VGTLGILIPPSIMLVLMADRMAMSVGDLFLGAVFPGVLLGGLYILYLVGYGMIRPEAA
                     PAAENEPIDMAAVWGLIRAILPPAFLILAVLGSIFFGLATPTEAAGVGAAGALLLAWI
                     NRRLGKGVMTNVLQETTRTTAFIFAVLLGATAFSLVLRGLGGDELIEDALLSLPFEAT
                     GILICILLATFILGFFLDWIELTLIILPLVSPVVTGLGFDPVWFTILFAVCLQTSFLT
                     PPVGFAIFYLKGVAPEGVDVRTIYRGVVPFIVLQLIGLLIIFNWKALVTWLPAQAYS"
     gene            complement(7907..8446)
                     /locus_tag="AC16QF_05735"
     CDS             complement(7907..8446)
                     /locus_tag="AC16QF_05735"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR6448088.1"
                     /translation="MLPETTFSRWADAWLVRIGQWASWLWLLLLIVIVTNVTLRYAFG
                     QGRIEFEEIQWHIYAAGFLLGLAYAYQADAHIRVDVLHERFSPRLQAWLELYGIILFL
                     LPFIALILIYAVPFVAISFTQSEVSLAPGGLPMRWLMKAMLPVGFGLLLLAVLSRLSR
                     VWAFLFFSDPDSDSEHHAG"
     gene            complement(8447..9571)
                     /locus_tag="AC16QF_05740"
     CDS             complement(8447..9571)
                     /locus_tag="AC16QF_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR6448089.1"
                     /translation="MTQRTMSQSAALVAVSVAFAVGIVGSLALRPPSGVGLNTPAAAT
                     TGETPLRIHWRMPVAFATNLPVLGDNALYVTGAIRAASNGALRIDPYEPNEIVPPFSI
                     TDAVRDRKVEAGYSWLGYDQGRIPASPLIGAVPFGMEPWEFSAWWFEAGGRELGEALY
                     AKQNTHILFCGISGPETAGWFRERIDSLEQMKGLKIRFAGLGGKVIEQIGASVTMLPG
                     GEIFQALEKGAIDATEYSLPIVDEKLGFNRIARYNYYPGWHQPSTAVHLIVNLDIWNA
                     LAYHDQQLIEMACTAGVTRNLARSEGLQGAVMARFPELGVSAETLPEEVLRELQRVSE
                     AVMLEQAEKDADFKKILESQQAFRSDYAIWKSRAYLPRNF"
BASE COUNT         1738 a         2911 c         3248 g         1804 t
ORIGIN      
        1 ggtggcccga tgataaagac gagggagcga acagtacaac actcgtacat aatcgggtgt
       61 ggatcttgat atctggatgg gagcccaacg actcgatgct tgaatattcc gaggcgcggc
      121 agatcagtga ttcggagcgg gtcgagtatc tggagttcgc tctggggctg gcggccgcgg
      181 ctggctctgc aattctgccg ttctttcgcg ccggcgcggg tgtggagaac aaacgcaccg
      241 atggtggatt cgacccggta acggaggccg atcacgctgc ggagcgcgtg atacgtgagc
      301 agattctcgg ccggtatccc gaacacggga tcttcggaga agaattcggt ttagacgcgg
      361 gtaacggtct gacctgggtc gttgatccca tcgatggcac tcgggcgttc atgagcggcc
      421 ttctgcattg gggggtgctg ctggcgctgt tcgatggcga gcgtcccgtc ctgggcgtca
      481 tgcatcagcc ttttaccgca gagttttttg cgggcgattg ccacaacgct cattaccacc
      541 ggggtgaaac ctcccaggcg ctcgaagtcc gggagtgcaa ggggctcggg caggccgttc
      601 tcggcacgac gtcgccgaag cttttcacgt cgccattcga gcggcggggc ctcgacgcca
      661 ttgaagaaag ggtgaaactc agccgctacg gtggggactg ctatctgtat gcgatgcttg
      721 ccatgggtca gctcgacatc gtcatggatg ccggcctgca gccttatgac gttcaggctc
      781 tggtgccgat catcgagggc gctggtgggg tcgtgaccac tgctcagggc ggtgacgcca
      841 gcatgggcgg gtttgtggtg gctaccggca gcgcggagct tcatgcagag gtgctcgcga
      901 ccatccgtgc gggcagtgaa ctcggccctg gttcctagat tcgaatcgag tggttcagat
      961 cagcggcccg tcatcgacgg gccggaatgt cgaggggacg tccgtgtcct gatcgtcggt
     1021 tcggcacgcc gggctgatgt gacccgaaaa cgcgactaaa cgggtcgatc agagcggacc
     1081 gatactgctg gtccgctcct cgagggcccg gcgcgaaccg gctggggcct cgagcgtcta
     1141 gactgccacc gaccgcctgc cgaagtgtga cgacaatgtc ggggttttgt agccgtttgg
     1201 cgcagtgctc ggtcaacgta tctgcggttc ctggaccgtc acggcagaag ttacggctcg
     1261 agatcctcga gggaggccgg aagtggccgt ccgccggcag tgtagtcgcc gtcgatgggc
     1321 acgatggtga tatagccctg ccggaagaga aaggtgtcgg cgttttcgat gtcctggctc
     1381 ggttctgccc ggccgaagtc cggggcgtag cgatcggggg ctgccgcttt gaagcccatt
     1441 gagtagtagg gcgcctttcc ctgggccgcg actcgcaccc cctttacggc ttcgggctcg
     1501 agcggaggat agttgacgtt cagagcaagc tcgcctttag accagggctg cgaagcacag
     1561 aagagcctcc tcaccaaccg gctggtgaac ccggcgaccg tcttcagatg acgatggttt
     1621 tccggcgcgg cctcgtcttc ggcgatggga ttcgtgctca cggcgattcc cgggatcggt
     1681 cttgccagcg ccttcatggc ggcgatggtt gcgcccacgg tcccggagat cacggttgcc
     1741 ggccccagat ttgctccctc gttgatcccg gaaacgacca ggtccacagg ctgctccggg
     1801 gggaagatgg cggtcgcgcc gaccagcact gccgtagcag ggctcccgga gaccgcatag
     1861 acttcggtga actcctcgtc cgggattcgg ggggccgcga tgaggtcgat ggtgagcgat
     1921 gcgctgctgc cgctgtagtt gcggtccggc gcgacccgtc tgacgttgtg cccgctggct
     1981 tttagttcgc gatgcagagc gatgatcccg ggcgtatcga acccgtcgtc gttgacgagc
     2041 agtatgttca gcggcctgtc cgtgcacggt tgggccgcga acgcgaacat tggtgacagc
     2101 agaacaattg cgaagaatcg tcggtacatg atgcaaacag aggatggccg cagatcatca
     2161 tgccatatct cggtgacggg tttagatccg ttcacggaga cttcacgagt cggtggctag
     2221 tctcgaactc atggatgcca accaattgga ctactccccc attcgccgcg ccggcgttat
     2281 aggcgacgtg cacgccgagg atgagcgtct ggcgggggcg ttggaatggc tctcgggcca
     2341 ggacgtggac gtcatcctgt gcaccggcga cgtcgccgac ggcgtcggct gcgtcaatac
     2401 cgcctgcagg ctgctcagag acgctgatgt gctgacggtc gcgggcaatc acgatcgatg
     2461 gctgctgcag gaccgcgttc ggcacgtcgc ggacgcccac tttctggccg atctggacga
     2521 tgaaagccgc gattacctga ctgcgctgcc tcgttcgagg actttgaata cggtcgccgg
     2581 accgctcctt ttgtgtcatg gggtggtgga gaacgatctc gcgaaggtct ggcccggcac
     2641 ggaacgttcg ccgatcgagc gtagcacgga gatggacgat ctggttgctg cgagcacgca
     2701 ccggtttgtg gtcaacggac acatgcacta ccgggtgctg atcgactttg agggtgtggt
     2761 cctcgtcaac gccggcacca tcagagggcc tttctccggc gtcaccgtga tcgatttcga
     2821 aggagatttc gcggcggcct acgagcgtga tggcaatggg cgggcgcacc gcgttgcgga
     2881 gcacgagctg acccgcaatg gggagcgaag gatctggcgt gacacgcagg aatttgacgg
     2941 ttcctggcag ccggttaccc tgcaccctta gaggcgcgcc gcgaggccgt cccgttgcct
     3001 cagtatcccg aggcggccgg gcgagcggct aactcggcca gcgtcgtgac gatgagcttc
     3061 ctctccgcgc gcttgactcg ctcctcgagc acctccgccg tgtcgttctt ttcgacaggc
     3121 actcgaacct gggcgagaat gggcccggag tcatatgctt cgttcactac gtgcacggtg
     3181 gcgcccgatt ccgtctcacc cgcctcgatc acggcctcgt gaacccgttg gccgtagaat
     3241 ccctgcccgc cgaactttgg aagaagtgca ggatgggtgt tgatgatcct gttcctgaac
     3301 cgaccgagca tgcgcgggcc tagcctcttc atgtagccgg ccagaaggac ccagtctgcg
     3361 ttcgcttctt ccagcgaacg gacgatggcg gcgtcgagct catcgggatc ccgatgggta
     3421 tgggcgctca ggtgaagggt tgcgatgccg tggcggctcg cgcgtgccag cgcctgggat
     3481 ctgctgttgt tgctgaccac cagcaccagc tccgcgggca gcgttccgtt ctcgatggcg
     3541 tcaatgaccg cctggagaat cgatccgccg tgagatgcca gggccgcgat gcgcatcagg
     3601 ccgccacctt gaatattgcg gtgtgaaaga tctccgtggt gagttccctg cctctgcggt
     3661 acgccacgta cagcctcagg aactcgtggc ccttcctgcg ccatttctcc atgagcgcgg
     3721 ccctgacttc cacggtatcg ccaaccttga caagcgcccg gtgccggaca tcggagccaa
     3781 catgaatcca tgctggcatt gcgtactccc tcgtgagtgc ccgattggca gtgctgagaa
     3841 gccagtgggg gtgagcgcag aggcgataga tgggcagatg gtcggcaacc tcagcggcgt
     3901 actgcgcatt gccgtcctcg gtgatctgcc agtgccacgg ctcgaacgtt tcgttctcga
     3961 ccaccgcctc ccagctgatt tccgcacgtt ccgcggttct gcggggctcg tcgagcacgg
     4021 cggcggtgac cggctccggc atatctcgcg gttgctcggt atgaagctcc gccagcaggg
     4081 tgccggcgtc attgcggcag ctgacgagca acgcggaccc tcggctttcg cagtccagat
     4141 gaagtctgtc cccgtcgtag gccggcttca ggaaccgcac cgagctcacc gaccggccga
     4201 gccagtcctc acccagacgc tccaccacgg gatgcgtcag gtgcccgtag acggcgacgc
     4261 ccgggacgag ggcaccggta aagccatagc gttgcgcgac ctcgtcgctg tggatcctgt
     4321 tctcgtgagc ctgggagaaa tttctcgcca cgacctcgta ggtggtcatc tgtggccctc
     4381 tgtgccgatg gatgcgttgc cgcaacgtct gctgatcata cctctgaagc tctaggtccg
     4441 ttaggcagat ctgtctgtga tgggtcggct gcggcgcagt caaggtcgat ccgggtggtg
     4501 aaagcgcatt atagtgcggc gatgaatggt gatgcttcag tggcggcgct gcgcggcgtg
     4561 cgcctggtgg tcgtgactgg caagggcggc gtgggcaaga cgtcggtggc tgctgggatc
     4621 gcgagcgctt ccgccaggtc ggggctcaga accgttctcg tcaccctcga caccggagat
     4681 accaggcatc cgctgctcga cgttcccctc gagtatcagc cctatcaggc ggccccgcgg
     4741 ctttccgttt cgcggctgga cgcgctcgag gcagtgcggg agtacgtccg gcgaaacatg
     4801 ccgttctcgt cgctctacga gaacatcttc gacgggcacg cgtttcgtaa ttttgcttcg
     4861 gcagcacccg gcttcgagga gctcatgtct ctcggcaagc tctacgagct ctgcaccagc
     4921 ggggagttcg atcgggtggt tttcgatgcg cccgccaccg gtcacatgaa gcttctgctg
     4981 gacgtgcctg gggccacctt gcgggccgtg cacgtgggac cgctgaacca caacgcgggc
     5041 aagattcagg acctgctgct ggacggctcg cggacgcgcg tcgtcatcac caccctcgcg
     5101 gaagagatgg cggtggagga ggcgatggac ctcgagcgct gctgccgtaa cgatcatcgc
     5161 atggccgtag gcccgattgt cgtcaatcgc ttggtgcctg agcgattttc agtcgaggag
     5221 atcgcggcgc tgcagagtat ggaggcgagc acgctggagc tgcgcgaagc ggtggcggct
     5281 gtggttgccg agcatgccga gcagcgatct caggaggagg cgttgcgcgc gctgagtgcg
     5341 gccggcagcg acgtcgtgtc cgtgccgcgc atcgtgcagg catgctttga cccggcggct
     5401 ttcttggaag cgctggaggc tcacctgatg cccctgttcg gtaacgcggc atgagtgccg
     5461 cgctcaagct ggaaggcgtc gttgcggatc ggcggataat catctgctgc ggcagcggcg
     5521 gtgtgggcaa aaccacgacc ggggcggcgc ttggagtgct tgcggcccag ctgggcagat
     5581 cagcgctggt catgaccatc gatccggcgc atcggctggc tcaagccatg ggcctgcagg
     5641 cgttggggca cgaacctgcg gaaattgcgc tggaagcacc cggcaggctg tcggccatga
     5701 tgctcgatac caaacacacc ttcgaccggc tggtggagca atacgcgccc agcggtcagg
     5761 tcagagacgc cattttcgcg aacgagtact accgccagct ctcctcgacg ctcggagggt
     5821 cccgggaact cgtggccatg gagcgcgttc tcgaagctgc gacggaggag gactacgatc
     5881 tgctgatcgt cgacacgccg cctgctcacc atgcgctgga ctttctcgac gcgccgcggc
     5941 gtctgatcga cctgatagac ggttccctga cgaactcgct gctcaagcct tacggcatgg
     6001 ctgcgcgggc gcagttcaac ctgttccggc agtcatctgc ggtcactctc aagtttctcg
     6061 agcgcttgac cggtttcgag atgctggccg atctgtcgga gttcctgctg gcgttctcca
     6121 gcatgttcga cggtttcaag gagcggtccc atcgcgtcat ggagctcatg cgtgagtcgg
     6181 ggacgacttt tctgctgatc tgcgcgccgg aggaagggag cctggaacag gtggaacagt
     6241 ttcgctcgcg tctggagaag gattcactgg atatctgcgg cgtgctggtg aaccgggtgc
     6301 acctgatgga cccgcgggcg ggtgatctcg atgactcggt gcttgccagg atcgccagcc
     6361 tggacgtcgc cgaaccgcgc ggtgctttcc tggccgagcg agtcgcggcg gcctatgagg
     6421 atcgacgcac catggcaagg gcagacgccg gcgtgatggc agaccgactc ggagaagacc
     6481 tgccggtgca ggccgtgcca catttccatc gggacctgca cagcatggcg gatcttcagg
     6541 cgttcgcaga gaccgttcgg aactgactca gctgtaggcc tgggccggaa gccaggtaac
     6601 gagcgccttc cagttgaaga tgatcagcag gccgatcagc tgaagaacga tgaacggcac
     6661 cacgccccgg tagatggtcc gaacgtccac tccctccggt gccacgcctt tcaggtagaa
     6721 gatcgcaaag cctaccggcg gggttagaaa gctggtctgc aggcagacgg cgaacaggat
     6781 cgtgaaccaa accggatcga accccagccc cgtgaccacg ggcgaaacca gcggcaggat
     6841 gatcagcgtg agctcgatcc agtcgagaaa gaacccgagt atgaaggttg ccagcagtat
     6901 gcagatcagg atgcccgtgg cttcgaaggg caggctgagc agggcgtctt cgatgagctc
     6961 atctccgccg agacctcgca ggaccagcga gaaagcagtg gcgccgagca gcacggcgaa
     7021 gatgaaggcc gtcgttcgcg tggtttcctg cagcacgttg gtcatcaccc ccttgcccag
     7081 ccgacgattg atccacgcga gcagcaaggc cccggccgcg cccacgcccg ccgcctccgt
     7141 tggcgttgcg agcccgaaga agatgctgcc gagcacggcc aggatcagaa aggccggcgg
     7201 cagaatcgcc ctgatgagac cccacacggc ggccatgtcg atcggttcgt tttccgcggc
     7261 gggggccgct tcggggcgga tcatgccgta gccgacgagg tagagaatgt agaggccgcc
     7321 cagcagaacg ccgggaaaga cggcgcccag aaacaggtcg ccgacgctca tggccatgcg
     7381 gtcggccata agcaccagca tgatgctagg cggtatcagt attcccaggg tgcccaccgc
     7441 gcagacggtg ccggcggcga gcgcgcgatc gtaaccctgc ttgagcatag cgggcatccc
     7501 gagcagggcc agcagcacca cggaggcccc gatgatgccc gtggtcgccg cgagcagaag
     7561 cccgatcagc gtcaccgtga tcgcaagtcc tccgcgcagc ccgccgaaca gtcgtgcaaa
     7621 gctggtcatg agctggttgg caatgccgga ccggtccagc agcaggccca tgaagatgaa
     7681 catgggcaag gcgaccatca cccagttctc catcacgttc cagatccggt ccacggtgat
     7741 ggatgcgtag ttccagtcca cgccgatggc gaggttgaag tccacatcca gcacgatggc
     7801 gaatgcggtg aagaggacgg cgagccctcc gagtatccat gccacgggga agccggtgaa
     7861 gaccagggcg atgaagctca cgaacatgag cagggcgaga atctcgttag ccggcatgat
     7921 gttcgctgtc cgagtccggg tcgctgaaga agagaaaggc ccacactcga ctcagccgcg
     7981 agagcacggc caggagcagc agcccgaacc cgacgggaag catcgccttc atcagccacc
     8041 gcatcggcag gccgccgggc gccagggata cctcgctctg cgtgaagctg atggctacga
     8101 aggggaccgc gtagatcagg atgagggcga tgaacggcag caggaacagg atgatgccgt
     8161 acagctccag ccaggcctga agtctcgggg agaaccgttc gtgcaggacg tcgacgcgaa
     8221 tgtgggcgtc cgcctggtag gcgtacgcaa gccccaggag gaatccggcg gcgtagatgt
     8281 gccactggat ctcttcgaac tcgatgcgcc cctggccgaa ggcgtaacgc agggtcacgt
     8341 ttgtgacgat gacgatcagc agcagcagcc acagccagga agcccattgg ccgatgcgaa
     8401 cgagccaggc atccgcccac cgtgagaacg tggtttcagg cagcactcag aagttccttg
     8461 gcaggtacgc gcgggacttc cagatggcgt agtcggagcg gaatgcctgt tgcgactcga
     8521 gtatcttttt gaaatccgcg tccttctcgg cctgctcgag catgaccgct tcgctcaccc
     8581 tttgaagttc ccgcagcacc tcttcgggaa gcgtttccgc ggagacgccg agttctggaa
     8641 atctggccat gacggccccc tgcaacccct cggagcgggc gaggttgcgg gtgactcccg
     8701 ccgtgcaagc catctcgatc agctgctggt cgtggtaggc cagggcgttc cagatatcca
     8761 ggttgacgat cagatgcacg gccgtcgacg gctggtgcca gccgggatag tagttgtagc
     8821 gcgcgatccg gttgaagccg agcttctcgt cgacgatggg cagcgagtat tcggttgcgt
     8881 cgatggcgcc cttctctagc gcctgaaaga tctcgccgcc gggcagcatc gttacggacg
     8941 cgccgatctg ctcgatgact ttgccgccga gacccgcaaa gcgaatcttc aggcccttca
     9001 tctgctcgag ggaatcgatg cgctcgcgga accaacccgc cgtttccggc ccgctgatcc
     9061 cgcagaaaag gatgtgggtg ttctgcttgg cgtacagcgc ttcgccgagc tcccgcccgc
     9121 cggcctcgaa ccaccaggcg gagaactccc agggctccat gccgaagggg acagcgccga
     9181 tgagcggtga cgcgggaatg cgtccctggt cgtaacccag ccaggagtag ccagcctcca
     9241 cttttcggtc ccgcacggca tccgtgatgc tgaagggcgg cacgatctcg ttgggctcgt
     9301 aggggtcgat gcgaagggca ccgttggaag ccgcgcgaat ggcgcccgtg acgtacaggg
     9361 cgttgtcgcc gagcacgggc aggttggtcg cgaaggcgac gggcatgcgc cagtggatgc
     9421 gcagcggggt ttcccccgtc gtagcggcgg cgggggtgtt cagcccgaca ccggatgggg
     9481 gccgcagcgc caggctcccg acgatgccca cggcgaacgc gacgctaacg gcgacgaggg
     9541 ctgcgctctg gctcatggtg cgctgagtca atctcaattc ccccgaagat tccgcggcat
     9601 tgcggatcca ttgtgggagg agcgtcaggc agacgcaagg cgatcggagc gcctcgcgcc
     9661 tccgagaatc ccgttaacat gtgaattgtt ttcgcgacgg g
//
LOCUS       DBNHLM010000123         2202 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_316923, whole genome shotgun sequence.
ACCESSION   DBNHLM010000123 DBNHLM010000000
VERSION     DBNHLM010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_316923"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="AC16QF_05745"
     CDS             complement(<1..1253)
                     /locus_tag="AC16QF_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6448090.1"
                     /translation="MDLESIDLFDPDIQEDWFPAYRTLLEEAPVYRIPGTTTYVICKY
                     EDILTVVRDPQTFSNQPEEHGGEPLIEHEEARQYYIDHGLGRETGRTRWPLLGMDPPD
                     HRKYRVLIDKHMLSKSVLKRTQPFIEQTVDDLIDGFEHTGEIEFVKAFAEPLPVTIIT
                     AMIGFPLEDMPQLKEWSTAWTLPFRRGLTLEEEMDVARQGVEFQDYIKAIADERRKTP
                     KDDIITHLAQARYAGKRPLTDHEIASIVDNMYVGGNETTTFTLTSGMWVMLREPKIYQ
                     ALSEDPARIPTFADEVLRLESPTQGLYRTAVVDTEIRGVPIPKGSTVHIRYGAANRDP
                     DVFECPERLDLERENASRHLAFSQGEHGCPGAPLSRLEQVIAYEHLLARLPNLRLTPE
                     KNDFKHLPGFVLRGLKQLYLSFDAV"
     gene            complement(1330..>2202)
                     /locus_tag="AC16QF_05750"
     CDS             complement(1330..>2202)
                     /locus_tag="AC16QF_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR6448091.1"
                     /translation="PTLPARAERIRDLLEAGGSADGQFTPADFERMQLDLYDYRARDQ
                     IPGILMALEGREDDDLELAAAILRGWDCQATGESAAACLYYPFVDRVWQRSFMMAVLD
                     DDVLRGIPLAAPGLNRFDVDAFLAEGSPWRAHRELLFLTMAEVMKGVVQDVRKDLGEP
                     DTWAWGRLHQVRFWHSLRKHEPWTHMQVGPDPVGGTGTTLAMALHVGNGPGRDPGVDE
                     VPCRANHGAAYRLVVDLGDPEHCRFVICGGNGGRPESPHVTDHYPTWLAGGYHDVTLA
                     RDELRIESIWEMAP"
BASE COUNT          330 a          702 c          765 g          405 t
ORIGIN      
        1 cagacggcgt cgaacgaaag atagagttgc ttcagcccgc ggaggacgaa gccgggcagg
       61 tgtttgaagt cgttcttctc gggcgtcagg cgcaggttcg gcagtcgcgc gaggagatgc
      121 tcgtaggcga tcacctgctc cagccgggag aggggcgcgc cggggcagcc gtgctcgccc
      181 tggctgaagg ccagatgccg ggaagcgttt tcgcgctcga ggtccagccg ctccgggcat
      241 tcgaagacgt cggggtcgcg gttggcggcg ccgtagcgga tgtgcacggt agaacccttc
      301 gggatcggca cgccgcggat ttcggtgtcc accacggcgg tgcggtagag cccctgggtg
      361 ggcgactcca ggcgaagcac ctcgtcggcg aaggtgggga tcctggcggg atcttctgag
      421 agcgcctgat agatcttcgg ctcgcgcagc atgacccaca tgcccgaggt cagggtgaag
      481 gtggtggtct cgttgccgcc cacgtacatg ttgtccacga tggacgcgat ctcgtggtcc
      541 gtgagcggtc gcttgcctgc gtagcgcgct tgcgccaggt gggtgatgat gtcgtccttc
      601 ggtgttttgc gacgctcgtc ggcgatggcc ttgatgtagt cctggaactc cacgccctgg
      661 cgggcgacgt ccatctcctc ctccagagtc agcccgcggc gaaacggcag ggtccatgcc
      721 gtggaccact ccttgagctg cggcatgtcc tccagcggaa agccgatcat ggcggtgatg
      781 atggtcaccg gcagcggctc ggcgaaggcc ttgacgaact cgatctcgcc cgtgtgttcg
      841 aagccgtcga tgaggtcgtc cacggtctgc tcgatgaacg gctgggttcg cttgagcacg
      901 ctcttgctca gcatgtgctt gtcgatgagc acgcggtact tgcgatgatc cggcggatcc
      961 atgcccagaa gcggccagcg ggttcgcccg gtctcccttc ccagcccgtg gtcgatgtaa
     1021 tactggcgtg cctcttcgtg ctcgatgagc ggctccccgc cgtgctcctc gggctggttg
     1081 gagaaggtct gggggtcgcg aacgacggtg aggatgtcct cgtacttgca gatcacgtag
     1141 gtggtggtcc cggggatccg gtagacgggc gcttcctcca gcagggtccg gtacgccggg
     1201 aaccagtctt cctgaatatc agggtcgaag agatcgatgg attccagatc cacgtgagcc
     1261 tcctccttca cgtaggaacc gcatgcagcg tttgtcgggc cgccgcgctg gcgcggtcat
     1321 tatggccgct cacggcgcca tctcccagat cgactcgatg cgcagctcgt cgcgggccag
     1381 cgtcacgtcg tggtagccgc cggccagcca ggtggggtag tggtcggtca cgtgggggct
     1441 ctccggccgg ccgccgttgc ctccgcagat gacgaagcgg caatgctccg ggtcgccgag
     1501 gtcgaccacc agccggtagg cggcgccgtg attggcgcgg cagggcacct cgtcgacccc
     1561 gggatctctc ccgggcccgt tccccacgtg cagggccatg gcgagggtcg ttccggtgcc
     1621 gccgacggga tccggtccca cctgcatgtg cgtccagggt tcgtgcttgc gcaggctgtg
     1681 ccagaaacgc acctgatgca gccggcccca ggcccaggtg tcgggctcgc caaggtcttt
     1741 tctcacgtcc tgcacgacgc ctttcatgac ctcggccatg gtcaggaaca gcagttcccg
     1801 gtgcgcacgc catggtgatc cctcggcgag aaacgcgtcc acgtcgaagc ggttgaggcc
     1861 cggcgccgcc agcggaatgc cccgcaggac gtcgtcgtcc agcacggcca tcatgaagct
     1921 ccgctgccag acgcggtcca cgaacgggta gtagaggcat gcggccgccg actctccggt
     1981 tgcctggcaa tcccagccgc gcaggatggc cgcggcgagc tcgaggtcgt cgtcctcgcg
     2041 accttccagc gccatgagaa tcccgggaat ctgatccctt gcccggtagt cgtagaggtc
     2101 cagctgcatg cgctcgaaat cggccggggt gaactgcccg tccgcggagc cgcccgcttc
     2161 gagcaggtcg cgaatccgct ccgcgcgggc cggcagcgtc gg
//
LOCUS       DBNHLM010000124         2557 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_138064, whole genome shotgun sequence.
ACCESSION   DBNHLM010000124 DBNHLM010000000
VERSION     DBNHLM010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_138064"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /locus_tag="AC16QF_05755"
     CDS             <1..1583
                     /locus_tag="AC16QF_05755"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007977712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004065 - arylsulfatase activity [Evidence
                     IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HIR6448092.1"
                     /translation="EISQMKSSRRTSTTSILLALLFASMSAWSQDKPNILVIWGDDVG
                     WSNISAYNLGMMGYQTPNIDRIAEEGAVFTHYYAHQSCTAGRSSFILGQHPFRTGLLT
                     IGMPGADHGIPDWSPTIADLLKNHGYATGQFGKNHLGDRDEHLPTNHGFDEFFGNLYH
                     LNAEEEPETYHYPKDPEFRARFGPRGVIKSTADGQIQDTGPMTRKRMETADEEFLGSA
                     MDFIDRSVKADKPFFVWMNTTRMHVWTRLKAEAEGVTEMGLYPDGMAEHDGHVGLLLG
                     QLDRLGIADNTIVIYSTDNGAEKVTWPDGGTTPFHGEKGTTWEGGMRVPAVVRWPGTI
                     KPGTKIHDIMSHEDWLPTLLAAAGENDVVQKLKKGHRANGKRWRVHPDGYNFLPYLKG
                     EADAPREEIFYFGQNGDLDAVRWRDWKVSFATFDGNIATGIREVANWPVITHLRSDPF
                     EEMHYESSMYLRWMADNIWLFVPIQAKVQEFLSTMGDYPFQEGQSLNAAGINYRSLKA
                     LQILQQLNQKGVLSPPTN"
     gene            1585..>2557
                     /locus_tag="AC16QF_05760"
     CDS             1585..>2557
                     /locus_tag="AC16QF_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR6448093.1"
                     /translation="MVARRLRAAAALAALLLITGIATVEAASRPKLILQITVDQLRAD
                     LPTRYYERLGKGGFKYLWEKGIVFTNAHHAHANTETIVGHATLATGAHPSVHGMVGNL
                     WFDRETGFTTYNVEDGDHRLLTEAADVDDATEIDPTQRAARSEGRSPAAILVTTFADE
                     LRSNTNGRAKAIGVSVKDRGAISMAGHSGTAYWFSKATGGFVTSTYYLDRYPDWVSDF
                     NAAGPAAAYAGTAWELLREQDTYLFGEADDRPWETDVAGFGRVFPHAFGDPSSPYFAT
                     WLTLSPAGDRLVLDFAKRAIVEESLGQDATTDYLSVSFSSTDYVGHVF"
BASE COUNT          508 a          822 c          806 g          421 t
ORIGIN      
        1 tggagatctc acagatgaaa tcgagtagac ggacgtcgac cacgtcgatc ctgcttgcgc
       61 tgctgttcgc ctcaatgtcc gcatggtctc aggacaagcc gaacatcctg gtcatctggg
      121 gtgacgacgt gggctggagc aacatcagcg cctacaacct ggggatgatg ggctaccaga
      181 cgccgaacat cgaccggatc gccgaggagg gtgcagtgtt cacccattac tacgcccacc
      241 agagctgcac ggcaggacgg tcttcattca tcctgggcca gcacccgttc cgcacgggcc
      301 tgctgaccat cggcatgccc ggcgccgatc acgggatacc cgactggagc ccgaccatcg
      361 ccgatctgct gaagaaccac ggctacgcga cgggccagtt cggcaagaac catctgggtg
      421 atcgggacga gcacctgccg accaatcacg ggtttgatga gtttttcggc aacctctatc
      481 acctcaatgc ggaggaagag ccggagacct atcactatcc gaaggacccg gagttccggg
      541 cgcgcttcgg gccgcgagga gtgataaaga gcacggcgga cggccagatc caggacaccg
      601 ggcccatgac gcgaaagcgc atggagacgg cggacgagga gtttctcggg agcgccatgg
      661 acttcatcga tcgttcggtg aaggccgaca agcccttctt cgtctggatg aacacgacgc
      721 gcatgcacgt gtggacgcga ttgaaggcag aggccgaggg ggtcacggaa atggggctct
      781 acccggacgg catggccgaa cacgacggcc acgtgggcct gcttctcggt cagctggatc
      841 gcctcggcat cgccgacaac accatcgtca tctacagcac cgacaacggc gccgagaagg
      901 tcacctggcc ggacggcggc acgacgccgt tccacggcga gaagggcact acctgggaag
      961 gcggcatgcg ggtgcccgcg gtggtgcgct ggccggggac catcaagccc gggacgaaga
     1021 tccacgacat catgtcccac gaggactggc tgccgacgct gctggccgcg gcgggcgaaa
     1081 acgacgttgt gcagaagctg aaaaagggcc accgggccaa cggcaagcgc tggagggtgc
     1141 acccggatgg ttacaacttc ctgccttacc tcaagggcga agcggacgcg ccgcgcgagg
     1201 agatcttcta cttcggccag aacggcgatc tggatgcggt tcgctggcgg gactggaagg
     1261 tcagctttgc gaccttcgac ggcaacatcg cgacgggtat ccgggaagtg gccaactggc
     1321 cggtaatcac ccatctgcgt tcggatccgt ttgaagagat gcactacgag tcgtccatgt
     1381 atctccgctg gatggcggac aatatctggc tgttcgtgcc catccaggcc aaggttcagg
     1441 agttcctctc gaccatgggt gactatccgt tccaggaggg ccaatccctg aacgccgcgg
     1501 gcatcaacta ccgatcgctt aaagcgctgc agatccttca gcagctcaat cagaagggcg
     1561 tgctgtcacc gccgacgaac tgacgtggta gcgcgtcgat tgcgcgcagc ggccgccctc
     1621 gcggcgctgc tgctgattac cggcatcgca accgtcgagg cggccagccg gccaaagctg
     1681 attctccaga tcacggtgga tcagctcagg gccgatctgc cgacccgcta ctacgagcgg
     1741 ctcggcaaag gcggcttcaa gtatctgtgg gagaagggca tcgtcttcac gaacgcccat
     1801 cacgcacacg ccaacacgga aaccatcgtc ggccacgcca ccctggctac cggcgcgcac
     1861 ccctccgttc acggcatggt gggcaatctc tggttcgacc gggaaaccgg gttcacgacc
     1921 tacaacgtcg aggatgggga ccaccggctg ctcaccgagg ccgccgacgt cgacgatgcc
     1981 acggagatcg atcccaccca gcgcgccgcc cgcagcgaag gccgctcgcc cgccgcgatc
     2041 ctggtgacca cctttgccga tgagcttcgc agcaatacca acggtcgcgc gaaagccatt
     2101 ggcgtctcgg tcaaggatag gggcgccatc tccatggcgg gtcattccgg caccgcgtac
     2161 tggttttcaa aggcgacggg cgggttcgtc accagcacct attacctgga ccgttacccg
     2221 gactgggttt cggacttcaa cgccgccggg cccgccgcgg catacgccgg gacggcttgg
     2281 gagctcttgc gcgaacagga cacctacctg tttggggagg ccgacgatcg gccctgggaa
     2341 accgacgtgg cgggattcgg ccgggtattt ccccacgcct ttggcgaccc cagcagcccc
     2401 tacttcgcca cctggctgac cctgagcccg gccggtgacc ggctggtgct cgacttcgcc
     2461 aaacgagcca tcgtcgagga gtcgctgggg caggacgcga ccaccgacta cctgagcgtg
     2521 agcttttcct ccaccgacta cgttggccac gtcttcg
//
LOCUS       DBNHLM010000125        17943 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_256846, whole genome shotgun sequence.
ACCESSION   DBNHLM010000125 DBNHLM010000000
VERSION     DBNHLM010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 17943)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17943)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17943
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_256846"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="AC16QF_05765"
     CDS             complement(<1..1496)
                     /locus_tag="AC16QF_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6448094.1"
                     /translation="MEILRSALIGGVLAVAVLMPEFAVAEGEGAMIEELVVTARRRAE
                     SVQDVPGTVTAIGQSTIEGAGVERVEDFIRLTPGVTLVNTAEVGDTQVNIRGINGARD
                     AENSFAFIVDGILHTNPAAFNREYPGLQQIEVFKGPQGAIYGRNAAAGAVIVTTERPT
                     EEFTGSIKTSYANDDSLFVQGTVSGPIVEDELFFRLSGTYRDTDGFWRNELKNDSSTI
                     DRFKNYDIDGRLLWEPNDRLSVDMKGRYGEVDASAITFNATFHLPIFAEVANEPAAFE
                     DVNDHEFVFQHNVDSDNNQEALELSVKADYEFENGYTLTGWMLYSDIQNDFIADGTSA
                     AFGFFQGNDACLDSTATLNAQGVILPPPQILGASPVGIIFTPDFSGSFFGPYTPTTCD
                     GIQEQVRDQDDFSFELRLASPVDQALRWMGGVYFLTIDRQVGISLNTDSGEKPIRGLL
                     QTSGPNRTEALVYDDFESDVFAVFGQLEYDITDAVELSFALRYDREER"
     gene            complement(1568..2116)
                     /locus_tag="AC16QF_05770"
     CDS             complement(1568..2116)
                     /locus_tag="AC16QF_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6448095.1"
                     /translation="MAETAQTVPTLGVSSETMAAARQDIEYLRRWYAKATDLLGENTP
                     ESIAEGTKIYHRIFTADAYIRTSGATTQPFEATGPDAWAEVANTALESYVATQHLIGT
                     QIVDIHELTQDAGGTITSGRASMTSYLQAWHAGEENLWLFIGTYMDEVRFTPGVGWQI
                     HDMNLVQVSGEHRALGGPRPTP"
     gene            complement(2190..2675)
                     /locus_tag="AC16QF_05775"
     CDS             complement(2190..2675)
                     /locus_tag="AC16QF_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6448096.1"
                     /translation="MIRGIHHVAVSTPDLDRIVRFYCDVMGAEQVYEGGWEKGSQLID
                     GIVGLKDSSCRQAMLKLGNAYLEFFEYQTPEPRPGDPARPVCDHGYTHFAIDVTDIDS
                     EYERLKAAGVKFNCPPPDFEGGPIRATYGRDPDGNVIEIQEIKDTAHEFYLDAWSGGR
                     S"
     gene            2769..3575
                     /locus_tag="AC16QF_05780"
     CDS             2769..3575
                     /locus_tag="AC16QF_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6448097.1"
                     /translation="MATRHYDTIEVESEGQVDWLTLNRPESLNAINTPMVTELRDYFG
                     GLQENADTRIVVMRGAGRAFCAGLDIKQARSGEHDNPFGAGMGFQGYLAEVYIRMRRC
                     PQPIISLIQGPACGGGFAFVLASDIRIAGESARMNAAFIRIGLSACDMGVSYFLPRLV
                     GTSVASELMLTGRFIHAARALATGLVSEVVPDAELVDAARPYIDEMLTTSPMGLRLTK
                     EGLNMAVDAASLEAAMAIENRNQVLCSKTSDAREGMQAFLEKRAPEYTGA"
     gene            3580..4203
                     /locus_tag="AC16QF_05785"
     CDS             3580..4203
                     /locus_tag="AC16QF_05785"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF036807.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR6448098.1"
                     /translation="MALSVIGAGYGRTGTLSLKAALEQLGYLKCHHMIEVLQSPAQIE
                     GWMAAARGERVNWDELFAGYLASVDWPSCHFYRELADYYPQAKVVLTVRDPHAWYESM
                     NATTLRVIRRSMAEHPDRPSLGTELVVKGAFDGNIDSAAHAVEIFEAHTAEVMANIPS
                     ERLLVFDVREGWEPLCNFLQRDVPAAAFPRVNSREEFDGIFFGNRAD"
     gene            4232..5059
                     /locus_tag="AC16QF_05790"
     CDS             4232..5059
                     /locus_tag="AC16QF_05790"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448099.1"
                     /translation="MSEKIVLVTGGAQGIGKGLARACLQRGASVVITNLDSEIAATTV
                     AELSEHGPIRAIRCDATDRNAVDALFDDVWATEGPVDLAFCNAGAGGMGQILETPMAD
                     VHHQFSTNLYSAIHLAQSYIPRLVEANRAGHVMFTGSENSLVLPRANADLAMGIYGGT
                     KHALLAVAEWLRYELRDTAVTVSLLMPGPVLTERLAATFDALADDPDNPEIRGRFPEH
                     VERTLRERFISTERCAEIALRGLAQGLFFIPTQAYIREDVDARYRELTAAFDALDLS"
     gene            complement(5123..5344)
                     /locus_tag="AC16QF_05795"
     CDS             complement(5123..5344)
                     /locus_tag="AC16QF_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448100.1"
                     /translation="MTIIEHPFTGAIYRMNDEGNILVTQGDRQGVFRPDGRWVSGEIR
                     EADPQMCVWVSNVPASPGESDWHVTSDKT"
     gene            complement(5341..6702)
                     /locus_tag="AC16QF_05800"
     CDS             complement(5341..6702)
                     /locus_tag="AC16QF_05800"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6448101.1"
                     /translation="MAEAVKDLDPDNMVRSPGLTYQQLLDQETHPVPDVLRLQSPKDF
                     GDADISIERYISKEWHDREVERLWKRVWQFACREEDIPDPGDHYRYDIAGMSFLVVRT
                     ESGAIRTYPNACLHRGRMLKEFDGHASELRCPFHGFAWHLDGSLKDVPAKWDFPHIDD
                     DQFGLPEIPTAVWAGFVFINPDQQCAPFEDFISDMASQFERWDLGSLYKQAHVAKMMP
                     ANWKIAQEAFCEAYHVNGTHPQILRSLGDINSQVDVWENCARVITPSLTPSPLLEYDL
                     TDGDVVRAMLDIPEGQPVPELPEGMSPRAWSAAMARETLRPDAGDRVDEYCDAELVDN
                     LDYTVFPNFHPWGAFNRIVYRFRPNGNDHRSAIMECIMLAPFQGERPPPAKIRWLAED
                     EPWSSALGFLGRVFDQDAFNMPKVQQGLEATYKPGITLANYQESKVRWLHHKLTEWVE
                     DGE"
     gene            complement(6797..9883)
                     /locus_tag="AC16QF_05805"
     CDS             complement(6797..9883)
                     /locus_tag="AC16QF_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6448102.1"
                     /translation="MSRIAQWAIERPLYPWLIMAACFFGGLYGIETVGRLEDPNFPIK
                     TAYIITSYPGASAVEVEQEVTDVIEEALLELPYIDHIISKSVPGRSEVQVDLLEHYGE
                     GDVPQIWDELRRRVVEAAERMPPGAGLPLVEDDFGDVYGLLYAIAAPDYSPADIRDMS
                     RQISTTLKRVPGVAKVQTSGEIGEAIFVELNHERLVRLGLPVDAVFGSIAQENSVVEA
                     GSVSYGQRRIRIAPELAFDSVAAVGDMRVGRPGSVEIIRLGDIADISRGPVEVPLDII
                     RHNGRRVFTVGVSVVDGRNVVEVGEAVDARLEQMLGDLPLGVEVIPIYRQHVVVSQAI
                     DEFGRNLMLSVVTVVLALCVFMGWRAGTVVGSVLLLTVMGTICVMSFLGIELQRISLG
                     ALMIAMGMLVDNAIVVAEGMVIGVRRGFTPKEAAALSVRRTMFPLLGATVIGILAFAP
                     IGLSPDKSGYYLRSLFEVVAISLLLSWLLAITVVPLLGNYLLVGRETARPEELYSGWG
                     YAPYRALLGFGLRRAWLAVLVLLAVTFGCIWGFQYVKQGFFPGTNAPLFYVDYYLPQG
                     TDIHATSGGIENVERILRTYPEVTDISAFIGKGPSRFTSIVRPEQPNPAYAHLVVRIT
                     DVNLMDAVIRRARQAVAEQIFDADIQIRRGEFTSSGTSKIEARFSGPDPDVLRHLAEQ
                     ALEVYLAHDLVERKTDWRQPELQIVPRFDEDRARVAGVSRTDVSRALALATLGVEIGL
                     FRDGNKLLPIIARAPHEERLDVGALSDRLVWSPAQQAHIPMRQIVSSFELVAENNTIY
                     RRDRERTIQARANPPPGHNPTMVFNSIRPGVEAIELPPGYILEWGGEHESSEDAKRLL
                     SSKIPLTFGLMLFISILMFGKLRQPLVIWLTVPMTVCGVVVSLLVTDLTFTFTAFLGF
                     LSLSGMLIKNCIVLVDEIDKRMLEGAHSLTTIVEASVSRLRPVMLAAGTTIAGMSPLL
                     ADAFFREMAVCIMGGLTFATLLTLVAVPVFYRIAFRRQLSSAQQASPGVLAGQAGG"
     gene            complement(9887..10948)
                     /locus_tag="AC16QF_05810"
     CDS             complement(9887..10948)
                     /locus_tag="AC16QF_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6448103.1"
                     /translation="MRRFLILLVLVCAGCEPAAEIVVEEQAVRPARIFRVSSEGVTVE
                     NQFVARVEAAQTVDVSFEVSGPLAELPVREGQTIDKGTLVAALDPTDFQLAVREAEVQ
                     LKLARQDLQRKQKLLADRGISQSVVDDAQALHDLRSVRLAQAREALEDARIVAPFDAY
                     VARRYTDNYANVQAGDKIVRLNDLNEIHVVASVPESLAATGSADQLIGAHAVFPFRPD
                     QRYPLTFRENLGEADAVAQTYEVTFAMPPPPNLQILPGMTATLELEMMAAEGEGRTMN
                     IPTSALIADSDKRFIVWIYDPETQAVSKRTVEVGPAVGRGVAVVSGLANGELIVATGA
                     SQLQNGMKIRVLGDPLTEL"
     gene            complement(10945..11493)
                     /locus_tag="AC16QF_05815"
     CDS             complement(10945..11493)
                     /locus_tag="AC16QF_05815"
                     /inference="COORDINATES: protein motif:HMM:NF037679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6448104.1"
                     /translation="MSAAGLAKTKILSAAAAVVGRSGAAHLTIDAVAAEAKLSKGGVL
                     YHFPSKRAILEGMLDRLISRYSERISRFEAELGDEPDAAIRAVILADRGQDAEERSMA
                     LALLAAAAEDPELLAPARAVIGQWFEAVEQSSGNVQLGILLLLATEGLRFLDMLDLLP
                     AGGTRREQLGQTLLNVAEAGRL"
     gene            11620..12795
                     /locus_tag="AC16QF_05820"
     CDS             11620..12795
                     /locus_tag="AC16QF_05820"
                     /inference="COORDINATES: protein motif:HMM:NF015963.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HIR6448105.1"
                     /translation="MPKWNNWSGRLAAEPDQIAFVRSEDDAAAVVAAAKRKGHAVRTR
                     GACHSHAPLLVTDGTLVDLSGLSGVISANMETRQAWVWAGTPIYALGRALHDSGLALG
                     NQGDIDRQAIAGATATGTHGTGAALASLSASVAGARMVLADGSILDCGPDAERELWEA
                     ARLNLGAMGIITRLQLTLREPYRLREHGWTEPFDSLLPQIPHLAAGNRHFEFFWFPHN
                     DGAIVKTINETDDDPEYPLAEEGSRCAWSYEVLPNSRVQPHTEMEYSVPADRGPECLR
                     AIRELILSRFPDVRWPVEYRLLAEDDVWLSTAYGRPTATVSVHQDVALDETPYFRACE
                     EIFRSFDGRPHWGKVHYLEPDVLASIHPRWQDWWQARDRADPDGLFLNDYLAAWRPR"
     gene            complement(12809..13588)
                     /locus_tag="AC16QF_05825"
     CDS             complement(12809..13588)
                     /locus_tag="AC16QF_05825"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448106.1"
                     /translation="MPEEFPLPNFNTDLSGEVALVTGTTSGLGRRFALVLAKCGAKVA
                     LTGRRVERFADLEAEIKAFGGECFSTRLDMTDAQDAIDVVGRVEEALGTVTILVNNAG
                     IPDAQRAHKMSLDLINAVIDTNLRGPYILSCEVARRLIDAKRPGRMVNISSVGGFRYD
                     GNGAALYSITKSAIARMSECLAVEWSRFNINVNCIAPGAFRSEMMDGMMERMGDFSGS
                     FPRKRLCDPAQLDSTLLYLCAPSSECVTGTIIKVDDGQGSR"
     gene            13720..14190
                     /locus_tag="AC16QF_05830"
     CDS             13720..14190
                     /locus_tag="AC16QF_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR6448107.1"
                     /translation="MATTLTVNGKAFTSDADPGTPLIFVLRNDLGLMGAKLGCGAEQC
                     GSCTVLADGEPIHSCTASLESISGRCIETLESLSRDGQPDAIQQAFMDENAAQCGYCT
                     AGIILAAKALLSKRPDPTRAEIVEALNGHLCRCGAHPRIIRAIERAAAARGQTS"
     gene            14187..16307
                     /locus_tag="AC16QF_05835"
     CDS             14187..16307
                     /locus_tag="AC16QF_05835"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR6448108.1"
                     /translation="MTLPPSLARTPRLDRWLRLDPDGSVAICTGKVEIGQGIKTALAM
                     IAADELDVALYRVRVRTADTALTPNELITAGSLSVEDSGGAIRVACATARAKLLDAAA
                     AVLGVPRDSLDVEDGTVTSSLTNQRTDYWSLPYDDLFSVEIQEPLPLKNPDSYRVVGR
                     PAQRLDLPAKVLGHPAFVHDMSLPDMLHGRLVKPPLPGARLERFPDDFQAPGLVAVVR
                     DGSFVGVVAEREEQAVRAAERLSAQCTWHRAPLSPLPSQMAAHLRENVAASFPVIHGG
                     PVAEPVPPAPEIRGTAKRLSATYTRPFQMHGSIGPSAALAQLESGRLTVYSHSQGVEL
                     LKIALADVLGMAEDSVRVVHAEGAGCYGHNGADDVALDAALLARAVTPRPVLTKWTRA
                     DEHGFEPYAPATVIEMTASLDDRGQILHWDHQAFGLTHMGRPRPSPGHSNLQSAWWLS
                     SPIPPVPREPLMFPEAGIHRNLQPIYDFPDQRLVKHFVADGPLRTSSTRSLGAFANVF
                     AIESFMDECAGAAGRDPFDFRLAHLRDPRAREVLETLRERAPDPAGGGRGIAMARYKS
                     RQAYVGLLVDVDVLDDARVSLKRALIVADAGLVIDPDGLRNQLEGGFIQAASWALKEQ
                     VDWDQNGVASRDWESYPIITFSEIPEIETHLIDRRHEPALGAGEASTGPTPAAIANAI
                     GNAAGVRVRDIPFTAEALRRAAAE"
     gene            16391..17110
                     /locus_tag="AC16QF_05840"
     CDS             16391..17110
                     /locus_tag="AC16QF_05840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6448109.1"
                     /translation="MSADATPAAIGLLEGIATTRAIRRYLDEPIPDEALRDILFAATR
                     GPSGSNRQPFRFVVLTDSPKAKEAKRLIGSAARSMWSDKRRNDGYDQGSGADESSPKA
                     RMARTMQSYVDEFDRVPVLVLPCLVRYRAPTPMEGASVYPAVQNLLLAARALGYGGVI
                     TGFHGAVDAQLKALLGIPEEVFIAATVTLGRPRGAHGPVRRRPMRELVYGDEWGTPPA
                     WAEDPPGTRYTSAGPPKRKPT"
     gene            17127..>17943
                     /locus_tag="AC16QF_05845"
     CDS             17127..>17943
                     /locus_tag="AC16QF_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6448110.1"
                     /translation="MEADVIVLGTGAAGLTAALAAHGHGADVLVLEKSDKVGGTSAWS
                     GGMIWIPCNHHMRAAGIEDSREEALTYLSALSHDLIDPDLAEAFVDTGPEMVAWMESN
                     TPVQFQLVPDFPDYHPEHPGGKPGGGRSLECPLFAYETLEAHRDLVTVGYNYGTAPIT
                     MEESNLGRAVPIEVPEEERARRAERDERGCGQALMGRLFKGCLERNITVRTGFRAMRL
                     LVEDGSVKGVAGEGPDGPFEVIARGGVVLATGGFEWNHDLVRTFLRGPMTSPVS"
BASE COUNT         3263 a         5953 c         5666 g         3061 t
ORIGIN      
        1 tcgcgctcct cacggtcgta gcgcagggcg aaggagagct ccacggcatc ggtgatgtcg
       61 tactcgagct ggccgaagac ggcgaagacg tcgctttcga agtcgtcgta aaccagggcc
      121 tcggtccggt tcgggccgct ggtctgcagc aggccgcgga tcggcttctc gccgctgtcc
      181 gtgttcaggg aaatccccac ctgacggtcg atggtcagga agtagacgcc gcccatccag
      241 cgcagcgcct gatcgacggg cgatgcgaga cgcagctcga agctgaaatc atcctgatcg
      301 cgcacctgct cctggatgcc atcgcatgtt gtaggcgtat agggcccgaa gaaggaccct
      361 gagaaatccg gcgtaaagat gatgcccacg gggctcgcac cgagaatctg cggtggtggc
      421 agaatcacgc cttgggcatt cagggtcgct gtggaatcca gacaggcgtc attgccttgg
      481 aagaacccaa acgccgccga cgtaccgtcc gcgatgaagt cgttctgaat atcgctgtag
      541 agcatccagc ccgtgagcgt gtagccgttc tcgaactcgt agtcggcctt caccgagagc
      601 tccaacgcct cctggttatt gtccgagtcc acgttgtgct ggaagacgaa ctcgtggtcg
      661 ttgacgtcct cgaacgccgc cggctcgttg gcgacctcgg caaagatggg caggtggaag
      721 gtggcattga aggtgatggc ggaggcgtcc acctccccgt aacggccctt catgtccacc
      781 gaaaggcgat cgttgggctc ccagagcaaa cggccgtcga tgtcgtagtt cttgaaacga
      841 tcgatggtcg acgagtcgtt ctttaactcg ttgcgccaga agccgtccgt gtcgcgatag
      901 gtgccggaca ggcggaagaa gagctcgtcc tccacgatgg ggccactcac cgtgccctgg
      961 acgaaaaggc tgtcatcgtt ggcgtagctc gtcttgatgc tgccggtaaa ctcctcggtc
     1021 ggccgctccg tggtgacgat gaccgccccg gccgcggcgt tacggccgta gatggcgccc
     1081 tggggcccct tgaacacttc gatctgctgc aggcccgggt actcgcggtt gaacgccgcc
     1141 gggttggtgt ggaggatgcc gtcgacgatg aacgcaaagc tgttctccgc gtcccgggcg
     1201 ccgttgatac cgcggatgtt gacctgggtg tcacccacct cagccgtatt taccagcgtc
     1261 acacctggag tgagccggat gaagtcctca acccgctcga ccccggcccc ctcgatggtg
     1321 gactggccga tggccgtcac ggttccgggc acgtcctgaa cggactccgc ccggcgccgg
     1381 gcagtcacca ccagctcctc gatcatcgcc ccttcgccct cggcgacagc gaactcaggc
     1441 atgagcacgg ctaccgccag caccccgccg atcaacgcgc ttcttaagat ttccatcgtt
     1501 tcttaccccc tattgaccct tgtgattctg accctcgtca ttcgggatca ggaacaccgc
     1561 tcctgaccta cggtgttgga cgcggccccc cgagcgcacg atgctccccc gaaacctgaa
     1621 ccagattcat gtcgtgtatc tgccacccca cgcccggcgt gaaccgaact tcgtccatgt
     1681 aggttccgat gaacagccac agattctcct cacccgcatg ccacgcctgc aggtagctgg
     1741 tcatcgacgc ccggccggac gtgatcgtcc cgcccgcatc ctgcgtcagc tcgtggatgt
     1801 ccacgatctg cgtgccgatc aggtgctgcg ttgccacata gctctccaga gcggtgttgg
     1861 ccacttccgc ccaggcatca ggccccgtcg cctcgaacgg ctgggtggtg gcgcccgacg
     1921 tcctaatgta ggcgtcagcc gtgaaaatcc ggtgataaat cttggtgcct tcagcgattg
     1981 actccggcgt gttctcacca agcaggtcgg ttgccttggc ataccagcgc ctcaagtact
     2041 cgatgtcctg ccgcgcagcc gccatcgtct cgctggacac cccgagcgtc gggacggtct
     2101 gagccgtttc cgccatcaca cttcctccca tcaacgtagc cgcgacaata gcgcgaacgc
     2161 ccgcaacttg gctagcaacg acatcgcctt caacttcgcc ctcccgacca ggcgtccagg
     2221 tagaactcgt gcgccgtatc cttgatttcc tgaatctcga tgacgttgcc gtccggatcg
     2281 cgtccgtagg ttgcgcgaat cggaccgcct tcgaaatcgg ggggcgggca gttgaacttg
     2341 accccggccg ctttgaggcg ctcgtactcc gaatcgatgt cggtgacgtc gattgcgaaa
     2401 tgggtatagc cgtgatcgca aaccggtctc gcaggatctc ccggtcgggg ctcgggcgtc
     2461 tgatactcga agaactccag ataggcgttg cccagcttca gcatggcctg acggcaggac
     2521 gagtctttca agccgacgat cccgtcgatg agctgggaac ccttttccca acccccctca
     2581 tagacctgct ctgcccccat gacgtcacag tagaaacgaa cgatcctgtc cagatctggg
     2641 gtcgacacgg cgacgtggtg aatgcctcga atcaattttc atccccttca agtcgagatt
     2701 cacggacatt aacattgaaa ggcgaggtcg gagaacatcc gcaccggcct atcagcaggg
     2761 agtcgagcat ggccacacga cactacgaca ccatcgaggt cgaaagcgaa ggccaggtcg
     2821 actggctgac gctgaaccgg ccggagtctc tgaacgccat aaacacgccc atggtgactg
     2881 agctgcggga ttatttcggc ggtcttcagg agaacgccga cactcgtatc gtggtgatgc
     2941 ggggcgccgg gcgcgccttc tgcgccgggc tggacatcaa gcaggccaga tccggcgagc
     3001 atgacaaccc cttcggggcc ggcatgggct tccagggtta cctggccgag gtctacatcc
     3061 gaatgcgccg ctgcccccag cccatcatct cgctgatcca ggggccggcc tgcggcggcg
     3121 gctttgcttt cgtgctggcc tctgacatcc gcatcgccgg cgagagcgca cgcatgaacg
     3181 ccgccttcat tcgcatcggc ctttcggcct gcgacatggg cgtgagctac tttctgccac
     3241 gactcgtcgg cacctcggtg gcctcagagt tgatgctgac cggccggttc atccacgctg
     3301 caagggcgct ggccacgggg ctggtatccg aggtggtccc cgacgcggag ctcgtggacg
     3361 cggccaggcc gtacatcgac gagatgctga ccacatcacc catgggcctg cgcctgacca
     3421 aggagggcct caacatggcg gtggatgcag ccagcctgga agccgccatg gccatagaga
     3481 accgtaacca ggtgctctgc tcgaagacca gcgacgccag ggaaggtatg caggcatttc
     3541 tggagaaacg ggcgcctgag tacaccggcg cttaggcggg tggctctgtc tgtcatcggc
     3601 gccggttacg gccggacggg aaccctgtcg ctgaaagccg ctttggaaca gctcgggtac
     3661 ctgaagtgcc accacatgat cgaggtgctg caaagcccgg cgcagattga gggctggatg
     3721 gccgcggcgc gcggcgagcg ggtcaattgg gacgagctct tcgccggcta cctggccagc
     3781 gtcgactggc cctcctgcca cttctaccgg gagctcgccg actactaccc gcaggccaag
     3841 gtggtgctga ccgtgcgcga cccccatgca tggtacgaga gcatgaacgc gacgacgcta
     3901 cgggtcattc gtcgttcgat ggccgagcac ccggaccgcc ccagcctggg caccgagctg
     3961 gttgtgaaag gcgctttcga cggaaacatc gacagcgccg cccacgccgt cgagatcttc
     4021 gaagcccaca ccgccgaggt gatggccaac attccctccg agcggctgct ggtgttcgac
     4081 gtccgcgaag gatgggagcc actgtgcaac tttctgcagc gcgacgtgcc cgccgctgcc
     4141 ttcccacggg tcaattcccg ggaagagttc gacggcattt tcttcggcaa tcgcgccgat
     4201 tgactgcaac cgaggtatga aaatggaatc gttgagcgaa aaaatcgtcc tggtcaccgg
     4261 cggcgcccag ggtatcggca aaggcctggc gcgcgcctgc ctgcaacgcg gtgcgagcgt
     4321 ggtgatcacg aacctggact ccgagatcgc ggcgacgacg gtagcggagc tgtccgagca
     4381 cggccccatt cgggccattc gctgcgatgc caccgaccga aatgccgtgg atgccctctt
     4441 cgacgacgtc tgggccaccg agggccccgt cgacctggcg ttctgcaacg ccggcgcagg
     4501 gggcatgggc cagattctcg agacccccat ggcggacgtg caccaccagt tctccaccaa
     4561 tctctactcg gccattcacc tggcgcagag ctacatccca cgcctggtcg aggcgaaccg
     4621 cgccggccac gtcatgttca ccgggtcgga gaactccctc gtcctgccgc gggccaatgc
     4681 ggatctggcc atgggtatat atggagggac caagcacgcc ctgctggccg tcgcggaatg
     4741 gcttcgctac gagttgaggg acacggccgt caccgtcagc ctgctcatgc cggggccggt
     4801 gctgaccgaa cggctcgcgg ccaccttcga cgccctggcc gacgacccgg acaacccgga
     4861 gatccggggc cggtttcccg agcacgtgga gcgcaccctg cgggagcgtt tcatctcaac
     4921 cgagagatgc gcggagatcg ctttgcgcgg cctcgctcag ggactcttct tcatcccgac
     4981 ccaggcgtac attcgagagg acgtggacgc acgctaccgc gagctcactg ccgcgttcga
     5041 cgccctggat ctgtcctgat ccgccctctt ctcacccgcc atgcgcctgc gccacggggc
     5101 gcggcggcga ttgctccggc ggtcaggtct tgtccgaggt aacgtgccag tccgactccc
     5161 cggggctggc cggcacgttg gacacccaga cacacatctg cggatcggcc tcgcggatct
     5221 ccccggagac ccatcgtccg tcaggccgga aaacaccctg gcgatcgcct tgcgtgacca
     5281 ggatgttacc ttcgtcgttc atccggtaga tggcaccggt gaacgggtgc tcgatgatgg
     5341 tcactcgccg tcctccaccc actcggtgag cttatggtgc agccagcgca ccttggactc
     5401 ctggtagttc gcaagggtga tgccgggctt gtaggtcgcc tcgaggccct gctgcacctt
     5461 gggcatgttg aacgcgtcct gatcgaagac ccggccgaga aagccgagcg ccgatgacca
     5521 gggctcgtct tcggccagcc agcggatctt cgccggaggc ggtcgttctc cctggaacgg
     5581 ggcgagcatg atgcactcca tgatggccga cctgtggtcg ttgccgttcg gccgaaagcg
     5641 atagacgatg cggttgaacg cgccccacgg gtgaaagttg ggaaacaccg tgtagtccag
     5701 gttgtctacc agctccgcat cgcagtactc gtctaccctg tcgcctgcgt ccggacgcaa
     5761 ggtctcccga gccatggccg cgctccaggc cctcggcgac ataccttccg gcagctccgg
     5821 gacgggctgg ccttccggga tatcgagcat ggcgcgcacc acgtcgccgt cggtcaggtc
     5881 atactcgagc aaggggctgg gagtcaggga gggcgtgatg acgcgggcgc agttctccca
     5941 gacgtccacc tggctgttga tgtcgcccag gctcctcaag atctgcggat gcgttccgtt
     6001 gacgtggtat gcctcgcaga aagcttcctg ggcgatcttc cagttagccg gcatcatctt
     6061 ggccacgtgg gcctgcttgt aaaggctgcc caggtcccag cgctcgaact ggctggccat
     6121 gtcgctgatg aaatcctcga acggcgcgca ttgctggtcc gggttgatga agacgaaacc
     6181 ggcccagacc gccgtcggta tctccggcag gccgaactga tcgtcatcga tgtggggaaa
     6241 gtcccatttc gcgggaacgt ccttgagcga cccatccaga tgccaggcga agccgtgaaa
     6301 cgggcagcgc agttccgagg catggccatc gaactccttg agcatgcgcc cccggtgcag
     6361 gcaggcattg ggatacgtcc tgatggcgcc gctttccgtc cggaccacga gaaacgacat
     6421 tcctgcgatg tcgtaacgat agtgatcgcc ggggtcggga atgtcttcct cccgacaagc
     6481 gaactgccac acgcgcttcc acagccgctc cacctcacgg tcatgccact ctttggagat
     6541 atatcgctcg attgagatgt cggcatcgcc gaaatccttc ggcgactgca gcctcagcac
     6601 gtcgggaacc gggtgggtct cctgatcgag cagctgctga tatgtgaggc ccggcgagcg
     6661 gaccatgttg tccggatcca gatctttgac ggcttcggcc attgcgtttc cccaagaaga
     6721 gtaagcgatc gggcgatgcg catcgccctc gcgctacaga ttcgccgtgc tcaccacgag
     6781 tgtcaaggcg ctcccgtcag cctcctgcct ggccggccag gacacccgga gaggcttgct
     6841 gcgcggagga aagctgacgc ctgaaagcga tgcggtagaa caccggcacg gccaccaacg
     6901 tcaacaacgt agcaaacgta agccccccca tgatgcagac cgccatctcc cggaagaagg
     6961 cgtcggccag caacggcgac atgcccgcta tagtggtccc ggcggcgagc atcaccggcc
     7021 gcagccggct cacgctggcc tcgacgatgg tcgtcaggct atgggcgccc tccagcatcc
     7081 gcttgtcgat ctcgtccacg aggacgatgc agttcttgat cagcatgccg gacagactca
     7141 gaaaaccgag gaacgccgtg aacgtgaagg tgagatcggt aaccagcagg ctgacgacga
     7201 ccccgcagac ggtcatgggg acggtaagcc agatcaccag gggctgtcga agcttgccga
     7261 acatgagaat gctgatgaac agcatgaggc cgaaggtgag cggaatcttg ctggacagca
     7321 gtcgcttcgc gtcctcgctc gattcgtgct caccgcccca ctccaggata tatccgggcg
     7381 gcagctcgat ggcttcaacg ccgggccgta tggagttgaa caccatggtg gggttgtgtc
     7441 ccggcggcgg gttcgcccgc gcctggatcg ttcgctcacg gtcgcgtcgg taaatcgtgt
     7501 tgttctccgc caccagctcg aagctggaga cgatctggcg catgggtatg tgggcttgct
     7561 gcgcggggct ccacacgaga cggtccgaca gggctccaac gtccagcctc tcttcgtggg
     7621 gcgcccgggc aatgatgggc agcagcttgt ttccgtcccg gaagaggccg atctcgacgc
     7681 ccagcgtggc cagcgcgagc gcgcgggaaa cgtccgtccg ggagacgccc gccacccgcg
     7741 cgcgatcctc gtcgaagcgc ggaacgatct gcagctccgg ttgccgccag tccgtcttcc
     7801 gttcaaccag gtcgtgggcg aggtatacct ccagcgcctg ctctgccaaa tgcctgagca
     7861 cgtccggatc cgggccggag aaccgcgctt cgatcttcga ggtcccgctg gaggtgaact
     7921 cgccgcgccg tatctggatg tcggcatcga aaatctgttc tgccaccgcc tggcgggcac
     7981 gtcgaatcac cgcgtccatc aagttgacgt cggtgatccg caccaccaga tgagcgtacg
     8041 ccggatttgg ttgctcaggg cgcacaatcg acgtaaagcg ggatggtccc ttacctataa
     8101 aagctgatat atcagtaact tctggatatg ttctaagtat cctttcgacg ttttcaatac
     8161 cgccggaagt ggcatgaatg tccgttccct gaggcaggta gtagtccacg tagaacaggg
     8221 gcgcgttggt gccggggaag aagccctgct tgacgtactg gaatccccag atgcagccaa
     8281 aggtcaccgc caggagtacc agcaccgcca accaggcacg gcgcaggccg aagccgagaa
     8341 gggcccggta cggcgcgtac ccccagcccg agtagagctc ctccggccga gcagtttccc
     8401 ggccgaccag gaggtagttg cccagcagag gcaccacggt tatcgccagc agccagctca
     8461 gcagcagcga gatggcaacc acctcgaaca gggagcgcag gtagtagccg gacttgtcgg
     8521 gcgagagccc gatgggagcg aaggcgagta tgccgatgac ggtggcgccg agcagaggaa
     8581 acatggttcg acggaccgac agcgcagcgg cttccttggg ggtaaagccc cgacggacac
     8641 cgatgaccat gccctcggcg accacgatgg cgttgtccac cagcatcccc atggcgatca
     8701 tgagcgcgcc cagggagatg cgctgcagct cgatgccgag aaacgacatg acgcagatgg
     8761 tgcccatgac cgtgagcagc agcacgctac ccaccaccgt tcccgcgcgc catcccatga
     8821 agacgcagag cgcgagcacc accgtgacga ccgacagcat caggttgcgg ccaaactcgt
     8881 cgatggcttg gctgaccacc acgtgctggc ggtatatggg aatcacctcc accccgaggg
     8941 gaagatcgcc cagcatctgc tccagccggg cgtccaccgc ttcgcccacc tcgaccacgt
     9001 tgcgcccgtc caccacggag acgccgacgg tgaatacccg gcggccgttg tggcggatga
     9061 tgtccagtgg aacctctacc ggtccccggg aaatgtctgc gatgtcgccg agccggatga
     9121 tctccacgga gcccggccgg cccacccgca tgtcgccgac ggcggccacg gaatcgaagg
     9181 cgagttccgg cgcgatgcgg attcggcgtt ggccgtagga aacggatccg gcttccacca
     9241 cgctgttctc ctgggcgatg ctgccgaaca ccgcatcgac cggaaggccg agccggacca
     9301 ggcgctcgtg attcagctcc acgaagatgg cctcgccaat ctcgccgctg gtctgcacct
     9361 tggccacacc cgggacgcgc ttgagcgtgg tgctgatctg gcgggacatg tcacggatgt
     9421 cggccgggct gtagtcgggc gctgcgatgg cgtagagcag gccatagacg tcaccgaaat
     9481 cgtcttctac gagcggcagc cccgcacccg gcggcatgcg ctcggccgcc tcaacgactc
     9541 ggcgcctgag ctcgtcccag atctgcggca cgtctccctc gccgtaatgc tcgagcaggt
     9601 ctacctgcac ttccgaccgg ccggggacgg acttcgagat tatgtggtcg atgtagggaa
     9661 gctccagcag ggcttcctcg atgacgtcgg tgacctcctg ctccacctcc actgccgagg
     9721 ccccggggta ggacgtgatg atgtaggcgg tcttgatcgg gaaattcgga tcttcaagcc
     9781 tcccaacggt ctctatgccg tagaggccac cgaagaagca ggcggccatg atcaaccagg
     9841 ggtagagcgg tcgttcgatg gcccattgcg cgatgcggct catgattcag agctccgtca
     9901 gcggatcgcc cagcacgcgg atcttcatgc cgttctggag ctggcttgcg ccggtggcaa
     9961 cgatcagctc gccgttggcc agcccggaaa ccaccgcaac tcccctgccc acggccggac
    10021 ccacctccac cgtccgtttc gacacggcct gggtttcggg atcgtagatc cagacgatga
    10081 agcgcttgtc ggaatccgcg atcagcgcag aggtgggaat gttcatcgtc cgaccttcgc
    10141 cctctgctgc catcatctcc agctcgagcg tggccgtcat gcccggcagg atctgaagat
    10201 tcggtggggg cggcatggca aacgtcacct cgtaggtctg cgcaaccgca tcggcttcgc
    10261 cgaggttttc cctgaaggtc agcggatagc gctgatccgg acggaagggg aaaacggcgt
    10321 gagcgccaat cagctgatcg gccgaaccgg tcgcagccag gctttccgga acgctggcaa
    10381 ccacatgaat ctcgttcagg tcattgagcc ggacaatttt gtcccctgcc tggacgttgg
    10441 cgtaattgtc cgtataccgg cgcgcgacgt acgcatcgaa gggcgcgacg atgcgggcgt
    10501 cctccaacgc ctcccgtgcc tgggcaagcc ttacgctgcg cagatcgtgg agtgcctgag
    10561 catcgtcgac cacggactga gagataccgc ggtccgccag cagcttctgc ttgcgctgca
    10621 gatcctggcg cgcgagcttg agctggactt cggcttcccg caccgcaagc tgaaagtccg
    10681 tggggtccag ggcagcgacc agggtgcctt tgtcgatggt ctgtccttcc cgaacgggga
    10741 gctcggcgag cggccccgac acctcgaagg acacgtccac cgtctgcgcg gcttccaccc
    10801 gagccacgaa ctgattctcc acggtgacgc cttcgctgga aacccgaaaa atccgggccg
    10861 gccgaacggc ctgttcctcg acgacgatct cggcagccgg ttcgcaacca gcgcagacca
    10921 gcaccagcag gatcaggaag cgcctcacag ccgtccggcc tcggcgacgt tgagcaacgt
    10981 ctgcccgagt tgctctcggc gggtgccgcc cgcgggcagg aggtcgagca tgtccaggaa
    11041 tcgcagacct tccgtggcaa gcagcaggag aatgcccagc tggacgttgc cggacgattg
    11101 ctccacggcc tcgaaccatt gcccgatgac ggcccgcgcc ggcgccagga gctccgggtc
    11161 ctcggcggcg gcagccagca gcgccagcgc catggagcgt tcctcggcgt cctgcccgcg
    11221 gtcggccagg atgaccgccc ggatggccgc atccggctcg tcgcccagct cggcctcgaa
    11281 acggctgatc cgctccgaat agcgggatat gagacggtcc agcatgccct ccagaatcgc
    11341 gcgcttgctg ggaaaatgat agagcacgcc gcctttggag agcttcgcct cggcggcgac
    11401 ggcatcgatg gtcagatggg cagcaccgct gcggcccacc accgccgcgg cggcgctgag
    11461 tatcttcgtt ttcgccagac ctgcagcact catggccgca aacgtaccgc aaaaaaacta
    11521 taccgtccag acggtatagt ttcgttgcgt agaatgcggc gctaccgtat gcgcagatcc
    11581 agccccacga cgggcagagg acacgcaaag gacacgcata tgccgaagtg gaacaactgg
    11641 tccggccgtc tcgcagccga gcccgatcag atcgcgttcg ttcgcagcga agacgatgcc
    11701 gccgccgtgg ttgccgcagc aaagcggaag ggtcacgcgg tgcgcacgcg gggcgcctgc
    11761 cacagccacg ccccattgct ggtcacggac gggaccctgg tggacctgtc gggtttgagc
    11821 ggcgtgatct cggccaacat ggagacgcgc caggcgtggg tgtgggcggg caccccgatc
    11881 tacgccctcg ggcgagcgct gcacgactcg ggacttgccc tgggcaatca gggcgacatc
    11941 gaccgtcagg ccatcgccgg agccaccgca acgggcactc acggcacggg ggccgctctc
    12001 gccagcctgt ctgccagcgt ggcgggagca cgcatggttc tcgccgacgg gagcatccta
    12061 gattgcggac ccgatgccga gcgggagctg tgggaggcgg cccgcctgaa tctgggcgcc
    12121 atgggcatca tcacccggtt acagctgacg ctgcgcgagc cttaccggct gcgggagcac
    12181 gggtggacag agccgttcga ctcgctgctg ccgcagatcc cgcaccttgc agcaggaaac
    12241 cgccacttcg agtttttctg gtttccccat aacgatgggg cgatcgtcaa gaccatcaac
    12301 gagacggacg acgaccccga gtacccgctg gcagaggaag ggtcccgctg cgcctggagc
    12361 tacgaggtgc tgccgaactc acgcgtgcag ccgcacaccg aaatggagta cagcgtcccc
    12421 gcagaccggg gccccgagtg cctgcgcgcc atacgggagc tcattctcag caggttcccg
    12481 gacgtgcgct ggcccgtgga gtaccgcctc ctcgccgagg acgacgtgtg gctgtccacg
    12541 gcttacggcc gcccgactgc caccgtgtcc gttcaccagg acgtcgcgtt ggacgagaca
    12601 ccctacttcc gcgcctgcga ggaaatattc cgcagcttcg acggtcgtcc ccattggggc
    12661 aaggttcact acctggaacc cgacgtgctg gcgagcatcc acccgcgctg gcaggactgg
    12721 tggcaggccc gcgaccgagc cgatccggac ggcctgttcc tgaacgacta cctcgccgct
    12781 tggcggcccc gatagcgaag cgatccggtc agcgcgagcc ctggccgtcg tccaccttga
    12841 tgatggtgcc ggtgacgcac tccgatgacg gcgcgcacag ataaagcagg gtgctgtcca
    12901 gctgagccgg atcgcacagc cgcttgcgcg ggaagctgcc ggagaagtcg cccatccgct
    12961 ccatcatccc gtccatcatc tccgacctga aagcgcccgg cgcgatgcag ttgacgttga
    13021 tgttgaatcg agaccactcc acggccaggc actcgctcat gcgcgcgatg gccgatttgg
    13081 taatggagta aagggcagcc ccgttaccgt cgtagcgaaa cccgcccacg gagctgatgt
    13141 tgaccatgcg gccgggacgc ttggcgtcga tgagtcgacg cgccacctcg caggaaagta
    13201 tgtacggacc ccgcaggttc gtatcgatga cggcgttgat cagatccagg ctcatcttgt
    13261 gcgcacgctg ggcatccggt atgccggcgt tgttcacgag tatcgtcacg gtgccgagcg
    13321 cttcctccac ccgtccgacg acgtcgatgg cgtcctgcgc atcggtcatg tccaaccgcg
    13381 tggaaaaaca ctccccgccg aaggccttta tttctgcttc caggtcggca aagcgctcta
    13441 cgcgcctgcc ggtgagcgcc actttggcgc cgcatttggc gagcaccagg gcgaagcgac
    13501 ggccgagccc ggacgtcgtg cccgtcacca gagccacttc cccgctgaga tccgtgttga
    13561 agttcggcaa aggaaactct tctggcatgt tggtctatcc ccagcgttga ttaaacgagg
    13621 caagttagca cttgtgctca ccgttcacta ccggcgatcc tcgcgagcaa cgatggcgac
    13681 agctggcata attctgaaaa cccctgcagg ttgccgaacg tggccacgac tcttaccgta
    13741 aacggcaagg ccttcaccag cgacgcggat ccgggcacgc ctctcatctt cgtgctgcgc
    13801 aacgatctcg gcctgatggg cgcgaagctc ggctgcggcg cggagcagtg cggcagctgc
    13861 acggtgctgg ccgacgggga gccgatccat agctgcacgg cgtcgctgga aagtatcagc
    13921 gggcgttgta tcgagacgct cgagtccctg tcccgggacg gccagccgga cgcaatccag
    13981 caggcattca tggacgagaa cgctgcccag tgcggctact gcaccgccgg catcatcctg
    14041 gctgccaagg cgctgctgag caaacggccg gacccgaccc gggccgagat agtcgaggcg
    14101 ctgaacggcc acctgtgccg ctgcggcgcg cacccgcgaa tcatccgcgc catcgagcgc
    14161 gccgctgccg cacgtggaca gacgtcatga cactgccccc atccctggcg cgaacgccac
    14221 ggctggaccg atggctgcgt ctcgacccgg acggaagcgt ggcgatctgc acagggaagg
    14281 tggagatagg acagggaata aaaacggcgc tggccatgat tgcggcggat gaactggacg
    14341 tcgcgctgta ccgcgttcgc gttcggacgg cagacaccgc tctcacgccg aacgagctca
    14401 tcacagcggg aagcctctcc gtcgaggaca gcggcggcgc catccgtgtc gcgtgcgcaa
    14461 cggctcgggc gaaacttctc gacgccgcgg ccgcagttct cggcgtgccc cgggactctc
    14521 tggacgtgga ggacggaacc gtaacgtcct ctttgacaaa ccagcgaacg gactactggt
    14581 ccctgcccta cgacgatctg ttctccgtgg agattcagga acccctgccg ctcaagaacc
    14641 ccgacagtta ccgggtagtc ggccgcccgg cacagcggct ggatctgccg gcaaaggtgc
    14701 tcggccatcc cgccttcgtc cacgacatga gcctgcccga catgctgcac ggccggctgg
    14761 tgaagccgcc cttgccgggc gccaggctgg aacgttttcc cgatgacttc caggcacccg
    14821 ggctcgtcgc cgttgtcagg gacggcagct tcgtcggggt ggtggccgaa cgggaggagc
    14881 aggccgtgcg cgccgccgag cgtctctcgg cgcagtgcac ctggcacagg gcgccccttt
    14941 cgccgctgcc ctcccagatg gccgcccatc tcagggagaa cgtcgcggcc agcttccccg
    15001 tcatccacgg cgggccggta gccgagccgg tcccgccggc acccgagatc cgggggaccg
    15061 ccaagcgctt gagcgcaacg tacacgcgcc cgttccagat gcacggatcc atcggtccgt
    15121 ccgcagccct ggcccagctc gagtccggac gcctcaccgt gtactcccac agccaaggcg
    15181 tcgagctgct gaagatcgcc ctggcggacg tgctcggcat ggccgaggac agcgttcggg
    15241 tcgtgcacgc cgaaggagcg ggctgctacg ggcacaacgg tgccgacgac gtcgctctgg
    15301 acgccgccct gctggcgcga gccgtgacgc cacggccggt gctcaccaaa tggacgcgag
    15361 cagacgagca tggctttgaa ccatacgcgc ccgccaccgt catcgagatg accgcgagcc
    15421 tcgacgatcg cgggcagatt ctgcactggg accaccaggc gttcggcctc acccacatgg
    15481 gccgcccgcg gccgagcccg ggacacagca atctgcagtc ggcgtggtgg ctgtcatcgc
    15541 caatcccgcc ggtgccgagg gagccgctca tgtttcccga ggcgggcatt caccggaatc
    15601 tgcagcccat ctacgacttc ccggatcagc gcctcgtcaa gcacttcgtg gccgacgggc
    15661 ccctgcggac gtcctctacc cgaagcctgg gtgcctttgc caacgtcttc gccatcgagt
    15721 ccttcatgga cgagtgcgcg ggcgccgccg gccgcgatcc gttcgacttc cgcctcgctc
    15781 acctgcgcga tccccgcgcc cgggaggtgc tcgagacatt gcgcgaacgt gcgcctgacc
    15841 ccgccggcgg cgggcggggg atcgctatgg cccgatacaa gagccggcag gcgtatgtgg
    15901 gcctgctggt tgacgtcgac gttctcgacg acgcccgcgt atcgctcaag cgggccctga
    15961 tcgtggccga cgcggggctc gtcatcgatc cggacgggct gagaaaccag ttggaaggcg
    16021 ggttcatcca ggccgccagc tgggcgctga aagagcaggt cgactgggac cagaacggcg
    16081 tcgccagccg ggactgggag agctacccca tcatcacttt cagcgagatt ccggaaatcg
    16141 agacccatct catcgaccgg cgccacgaac ccgcgctggg agccggagaa gccagcaccg
    16201 gcccgacgcc ggcggcgatc gccaacgcga taggcaacgc cgccggcgta agggtccggg
    16261 acataccgtt caccgccgag gccttgcgac gcgcggccgc cgagtagcgc tggggcgccg
    16321 tgagctacta tgttccccat ctcttctaca ccataatcga gtgccgccgc acgtccatca
    16381 ggaactcaca atgtccgccg acgccacgcc cgccgccatc ggcctgctgg aaggtatcgc
    16441 caccacgagg gccatccgcc ggtacttaga cgagcccatt cccgacgaag cgctgcgcga
    16501 catcctgttt gccgccactc gcgggccgag cggctccaac cggcagccgt tccgcttcgt
    16561 ggttctgacc gacagcccca aggcgaaaga agccaaacgc ctgatcggct ctgcggcgcg
    16621 aagcatgtgg tcggacaagc gccgcaacga cggttacgac cagggctcgg gcgccgacga
    16681 gagctccccg aaagcgcgca tggcccgcac catgcagtcc tacgtggacg agttcgaccg
    16741 ggttcccgtg ctggtgctac cctgcctggt acgctaccgg gcgcccacgc ccatggaagg
    16801 cgcttcggtg taccccgctg tgcagaacct gctgcttgca gccagggcac tgggctacgg
    16861 tggcgtcatc acgggcttcc atggcgccgt ggacgcgcag ctgaaggccc tgcttggtat
    16921 tcctgaagag gtgttcatcg ccgccaccgt gaccctgggt agaccacggg gtgcccacgg
    16981 tcccgtccgg cgaagaccca tgcgcgagct cgtctacggg gatgagtggg gcacaccgcc
    17041 cgcctgggcc gaggaccctc cgggaacccg ttacaccagc gcgggccccc caaagcgaaa
    17101 acccacctga ccgagtggag gtatcgatgg aagcagacgt catcgtgctg ggcaccggag
    17161 ccgccggcct gaccgccgct ctggcggcac acggccacgg ggcagacgtt ctggtgctcg
    17221 aaaaatcgga caaggtcggc gggaccagcg cgtggtccgg cggcatgatc tggatcccct
    17281 gcaaccatca catgcgcgct gccggcatcg aagactccag agaagaagcg ctcacgtatc
    17341 tatcggccct gtctcacgat ctcatcgacc ccgacctcgc cgaggccttc gtggacacag
    17401 gcccggaaat ggtcgcctgg atggagtcca acactcccgt tcagtttcag ctggtaccgg
    17461 actttcccga ctaccacccg gagcaccccg gcggaaagcc gggcggcgga cgttccctgg
    17521 agtgcccgct gtttgcctac gaaacgctcg aagcgcaccg cgatctggtc accgtgggct
    17581 acaactacgg caccgcgccc atcacgatgg aagaatccaa cctgggtcgc gcggtgccca
    17641 tcgaggtccc agaggaagag cgggcccgcc gggccgagcg cgacgagcgg ggttgcggcc
    17701 aggcgctgat gggacgactg ttcaaggggt gcctcgagcg gaacatcacg gtaagaacgg
    17761 ggttccgggc catgcgcctg ctggtggagg acggctcggt gaagggcgtc gcgggcgaag
    17821 gccccgacgg cccgtttgag gtcattgccc ggggcggcgt ggtgctggca acaggcggtt
    17881 tcgaatggaa ccacgatctg gtccggacct ttctgcgcgg accgatgacc agccccgtat
    17941 ccg
//
LOCUS       DBNHLM010000126         4829 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1035300, whole genome shotgun sequence.
ACCESSION   DBNHLM010000126 DBNHLM010000000
VERSION     DBNHLM010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1035300"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="AC16QF_05850"
     CDS             complement(<1..1200)
                     /locus_tag="AC16QF_05850"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6448111.1"
                     /translation="MEIVSQATPVNLTARYPELDNGPVRADVYWQPEIFDQEIERIFR
                     PTWHCVGREEQITATGEFFVKELPTFEFSVIVARGDDGAVRAFLNACPHRGNQVELTA
                     CGRRSAFTCKFHAWSYDLKGRLKSVPDEAGFPGLDKAAIGLTEFPCETWQGFVFVNLN
                     ENPPVSLIDYLGEQGADLEGYPFDQGTARFQFAGEVETNWKFLVDSFCETYHVPFLHR
                     RTIHSTMAGPDNPHGRVVDVRLKGDHRTTSVWGNRSYEPKTVQAMALTHTPGVSITSA
                     GADTVQLPRGLNQTRSPNWSIDVTVFFPGFVCVIGAGMYFTHQMWPLAANRTRWEMTG
                     YLRPAANAAERFGQEHAMVELRDAVLEDANTLERMQRNVATGLIRAFHFHDHELSLRY
                     QHHTVCRH"
     gene            1361..2182
                     /locus_tag="AC16QF_05855"
     CDS             1361..2182
                     /locus_tag="AC16QF_05855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase family protein"
                     /protein_id="HIR6448112.1"
                     /translation="MLDDYLDRIGHSGPLNADLATLTALHRAHITAIPYENLEIQLGR
                     ENTLDEKDIFTKLVARRRGGWCYEMNGLLTRALAQIGFQAQRVSGAVHRDEQGSVVEG
                     NHLVGLVELDRTWVVDVGLADGPLEPFPLEPRQWKEGALEFGLERLDSDWWRFHNHAH
                     GLARTFDFTKENRELAHFESMCRFLQTDPASPFVAFALASRRRENGFDALRDTTWFHV
                     EDGRLEKRKIEDEAEYSEVLEQILGNDLGEEAGVLWHRAEERSAAREAAQSATQD"
     gene            complement(2257..3648)
                     /locus_tag="AC16QF_05860"
     CDS             complement(2257..3648)
                     /locus_tag="AC16QF_05860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6448113.1"
                     /translation="MTGSSGGVDEAAWDELGSGVRGALLREGDAGYDEARSVWNGMMD
                     RHPAAVLQCLGVSDVMAGVNFARNHGLPLAVKGGGHNVAGKGVCDDGLTLDMSLMRSV
                     RVDPGTLRARAEAGALWRDFDHEAQTFGLTTTGGVVSSTGVAGLTLGGGIGYLTRTHG
                     LACDNLLAADVVTADGRLVQASENENPDLLWALRGGGGNFGVVTSLEFQLHEVGPDVA
                     AAIVFYPLAAGADVLHAYREYAEGAPDAVACYAMVVRGPADLPSEYAGQPVLAIIACY
                     SGAVAEGLRALDPVGSFATPIAATIEGTPYRAMQQMFDPGSPHGVRYYWKSQYLSGLP
                     DEFLETVVRFAREMPGAYTAIGIEPLGGAMERVDPAGTAYPHRRFPFNLGIWSGWDEP
                     SEDSGVISWTREFFEAVVPFGAGAYVNYLSDDEGGRVDEAYGDNYARLRQVKAQWDPG
                     NLFRVNQNIAPRG"
     gene            3777..>4829
                     /locus_tag="AC16QF_05865"
     CDS             3777..>4829
                     /locus_tag="AC16QF_05865"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6448114.1"
                     /translation="MAAQRTSQAEPATRLDVLIIGAGFSGVYQLYRLREQGFSVKIYE
                     AASGLGGIWRWNCYPGARVDSHVPNYEFSIREVWEDWNWTERFPGWAELRRYFEHVDA
                     KLDLSRDIRYNTWVTAARFDAEHDEWVVTTDDGAETRARFLITCIGFAAKPYVPDFPG
                     LESFTGPCHHTAPWPEGGLDLAGKRVGIIGTGASGVQVAQEAAAVASQLTIFQRTPMI
                     ALPMQQRQLDAETQDMLKAAYPARFRQRAVASSTMFDIVADERGAKEVSEEVRNAIFE
                     AAWQKGGFQFWFGTFSDILRDETSNRLAYDFWRDKTRARIRDPWLKEKLAPMEPVHPF
                     GTKRPSLEQNFYDLFNQ"
BASE COUNT          887 a         1607 c         1520 g          815 t
ORIGIN      
        1 gtgccgacag acggtgtggt gctgatatcg cagcgacagc tcgtgatcgt gaaagtggaa
       61 ggccctgatg agccccgtcg ccacgttgcg ctgcattcgt tcaagggtgt tggcatcctc
      121 cagcaccgcg tcgcgaagct ccaccatggc gtgttcctgg ccgaatcgct ccgccgcgtt
      181 ggcggcggga cgcagatacc cggtcatctc ccagcgggtc cgattggcgg ccagaggcca
      241 catctggtga gtgaagtaca tgccggcgcc gatgacgcag acgaaccctg gaaaaaagac
      301 ggtcacgtcg atggaccagt tcggcgagcg cgtctggttg agacctctgg gcagctgcac
      361 ggtgtcggcc ccggcgctgg tgatcgacac ccccggggta tgggtcaagg ccatggcctg
      421 caccgttttg ggttcgtaag accgattgcc ccataccgag gtggtccggt gatcgccctt
      481 caggcgcacg tccacgacgc ggccgtgggg gttgtccggc cccgccatgg tactgtggat
      541 ggttcgccgg tgcagaaacg ggacgtggta ggtttcgcag aacgaatcca ccagaaactt
      601 ccagttggtc tccacctcgc ccgcgaactg aaagcgtgcc gtgccctggt caaacggata
      661 accctcgaga tcggcgccct gctcgcccag atagtcgatg aggctcacgg gcggattctc
      721 gttgagattg acgaagacga agccctgcca ggtctcgcag ggaaactcgg tcagcccgat
      781 ggccgccttg tccagaccgg gaaagcccgc ctcgtcgggg acggacttga gccggccttt
      841 caggtcgtag ctccacgcgt gaaacttgca ggtgaacgcg ctgcggcgac cgcacgccgt
      901 cagctccacc tggttgcccc ggtggggaca ggcattgaga aacgcccgga cggcgccgtc
      961 gtcgcctcgg gcaacgatga ccgaaaactc gaacgtcggc agttccttga cgaagaactc
     1021 ccccgtcgcg gtgatctgct cctccctgcc gacgcagtgc caggtcggcc tgaagatacg
     1081 ctctatttcc tgatcgaaga tctcgggctg ccagtagacg tccgctcgga ccggcccgtt
     1141 atcgagttcc gggtaacggg ccgtcaggtt gaccggcgtg gcttgcgaca ctatttccat
     1201 ctcccctcgg cgacggctcg atactagtac aatcgccctc cggattgcag cgccggcggt
     1261 cagggcaccc gttcggctgc ggcgcccctt tgagcggaac tggttccaac cgggtccgca
     1321 tgctagcttg actgtcccat cggcgctccc atcgaaagac atgctagacg actatctcga
     1381 ccggatcggc cactcaggcc cgctgaatgc cgacctcgcg acgcttacgg cccttcatcg
     1441 tgcgcacatc actgccattc cctacgagaa tctggaaatt cagctgggcc gcgaaaacac
     1501 gctcgacgag aaagacatct tcacgaagct cgtggcgcgc cgccgcggcg gctggtgcta
     1561 cgagatgaac ggtctgctga cacgagcgct agcccagatc ggctttcagg ctcagcgcgt
     1621 cagcggagcc gtgcatcgcg acgagcaggg cagcgtcgtg gaaggaaatc acctggtcgg
     1681 cctcgtcgag ctggaccgca cctgggtagt ggacgtgggt ctggcggacg gcccgctgga
     1741 accctttcct ttagaaccgc gccagtggaa ggagggcgct ctcgagttcg gcctggagcg
     1801 gctggattcg gactggtggc gctttcacaa tcacgcccac gggctcgccc gcaccttcga
     1861 cttcacaaaa gaaaaccgtg agctggccca cttcgaaagc atgtgccgct tcctccagac
     1921 cgatccagcc tccccgttcg tggcgttcgc gttggcgagc cggcggcggg agaatgggtt
     1981 cgatgccttg agggacacga cctggtttca cgtggaagac ggtcgcctgg agaagcgcaa
     2041 gatcgaggac gaagcggaat actccgaggt gctggagcag attctcggaa acgatctggg
     2101 cgaggaagcg ggcgtgcttt ggcatcgggc cgaggagcgg tctgcggcgc gcgaggcagc
     2161 ccagagcgcg acgcaggatt gatggccgcc gtccgcgacc aggccaggtc cttagaccgg
     2221 ggcctggacg aaaatcctgc cgccgactcg agctgatcag ccccggggcg cgatgttctg
     2281 gttgacccgg aacaggttgc cggggtccca ttgcgccttc acctggcgca ggcgcgcgta
     2341 gttgtccccg tacgcctcat cgacccttcc accttcatca tcgctgaggt agttgacgta
     2401 ggcgcccgca ccgaaaggca ccaccgcctc gaagaactct ctggtccagc tgatgacgcc
     2461 agaatcctct gacggctcat cccagcccga ccagatcccc agattgaatg ggaaccggcg
     2521 atgggggtag gcggtgcccg ccgggtccac gcgctccatt gccccgccga ggggttcgat
     2581 gccgatggcc gtgtaagcgc cgggcatctc ccgagcgaaa cgcaccaccg tctcgaggaa
     2641 ctcatcaggc aatcccgaca ggtactggga cttccagtaa tagcgcacgc cgtgggggct
     2701 gccgggatcg aacatctgct gcatggctct atagggcgtc ccttcgatcg tggcggcaat
     2761 gggcgtggcg aatgacccga ccgggtccag cgcgcgaagg ccttcagcca ccgctccgga
     2821 gtagcacgcg atgatcgcca acacgggctg ccctgcatat tccgagggaa gatcggcggg
     2881 acctctgacc accatggcgt agcaggcgac cgcatccggc gccccttcgg catactcccg
     2941 atacgcgtgc aggacgtccg caccagcggc taacgggtag aagacgatgg ccgccgcgac
     3001 atcgggacct acctcgtgca gctgaaactc cagcgaggtg accacgccga agttgccccc
     3061 gcccccgcgc agcgcccaca gcaggtccgg attctcgttc tcgctcgcct ggacgagcct
     3121 gccgtcggcc gtgacgacgt ccgcggcgag caggttgtcg caggccagcc catgggttcg
     3181 cgtcaggtaa ccgatgccgc cacctaaggt cagaccggcg acgcccgtgc tggacaccac
     3241 gccgcccgtg gtggtgaggc cgaacgtctg cgcctcgtga tcgaagtcgc gccacagcgc
     3301 gcccgcctcg gcgcgggcgc ggagcgtccc gggatccact cgcaccgagc gcatgagcga
     3361 catgtccagg gtcagaccat cgtcgcagac gcctttaccg gcaacgttgt gaccaccgcc
     3421 tttcactgcc agcggcaggc catggtttcg ggcaaagttc acgccggcca tgacgtcgct
     3481 gaccccaagg cactgcagaa cggcggccgg atgccgatcc atcatgccgt tccagacgct
     3541 gcgcgcctcg tcgtagcccg catccccctc gcgaagcagg gcaccgcgca cgcccgaccc
     3601 aagctcgtcc caggccgctt cgtcgactcc tccgctggaa cccgtcatat cagcatcctt
     3661 tcgatggttg cactgcttgc cggactttag aggtcctcac atccacggac aagttggacc
     3721 cggccggcgc caacgtctta gactgggtgg gatccgcaga gagggagaag cgccccgtgg
     3781 ctgcccagcg aacgagccag gccgaaccgg caacccggct cgacgtcctc atcatcggcg
     3841 ccggattcag cggcgtctac cagctctacc ggctccgaga gcagggtttc agcgtaaaga
     3901 tctacgaagc cgccagcggt ctcggcggca tctggcgatg gaactgctac cccggcgccc
     3961 gggtggattc ccacgtgccc aattacgagt tctccatacg cgaggtatgg gaggactgga
     4021 actggaccga acgctttccg ggctgggcgg agctgcgccg ctactttgag cacgtggatg
     4081 cgaagctcga tctgtcccgg gacatccgct acaacacctg ggtgactgcc gcccgcttcg
     4141 acgctgagca tgacgagtgg gtcgtgacca ccgatgacgg cgccgagacc cgcgcacgtt
     4201 tcctgatcac ctgcatcggc ttcgctgcaa agccgtacgt tcccgacttt cccgggctgg
     4261 aaagcttcac gggcccctgc caccacaccg ccccctggcc cgagggcggt ctcgacctcg
     4321 cgggtaagag agtcggcatc atcggcacgg gcgcgagcgg cgtccaggtc gcccaggaag
     4381 ccgccgcggt tgcgtcccaa ctgaccatct tccagcgcac gccgatgatc gccctgccca
     4441 tgcagcagcg acagctggat gccgaaaccc aggacatgct gaaagccgcc tacccggccc
     4501 gattccggca gcgggccgtg gccagctcca ccatgttcga catcgtcgca gacgagcgcg
     4561 gcgcgaaaga agtgtcggaa gaggtccgca acgccatatt cgaagccgcc tggcagaaag
     4621 gcggctttca attctggttc ggcacgttca gcgacatact ccgagacgag acctcgaacc
     4681 ggctcgctta cgacttctgg cgggacaaga cccgggcgcg gatcagggat ccctggctaa
     4741 aggagaagct ggcgccgatg gaacccgtcc atcccttcgg caccaagcgt ccgtctttag
     4801 agcagaactt ctacgacctc ttcaatcag
//
LOCUS       DBNHLM010000127        12832 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_735445, whole genome shotgun sequence.
ACCESSION   DBNHLM010000127 DBNHLM010000000
VERSION     DBNHLM010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12832)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12832)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12832
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_735445"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="AC16QF_05870"
     CDS             complement(<1..512)
                     /locus_tag="AC16QF_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014619.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH/LACC1
                     family protein"
                     /protein_id="HIR6448115.1"
                     /translation="MVEANAWEWARWPDDAPGHRIAAVTTTRPGGFSKGKYAAFNLAC
                     HVHDDPAHVARNRRDLLKALGLAHVQWLEQVHGTGCVQATLASCDRVPVADAAWTSVP
                     GLALAVLTADCLPVVVAARDASTVAVIHAGWRGLVAGIIPSVVGSLPGRTTGYVGWIG
                     PGIGADAYEVG"
     gene            complement(505..1473)
                     /gene="rluD"
                     /locus_tag="AC16QF_05875"
     CDS             complement(505..1473)
                     /gene="rluD"
                     /locus_tag="AC16QF_05875"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HIR6448116.1"
                     /translation="MAEPLEIQKRVTVPPDVAGERVDKAAARLFPEFSRAALTAWIQD
                     GALTVDGARVKPKVKLVGGETLVLDAVVAPREDWSTGEPVPFDVLYEDADLLVVNKPA
                     GLVVHPGAGNPDGTLVNGLLHHRASLALVPRAGIVHRLDKDTSGLLLVAANLEAHAAL
                     VRALQARQIQRGYRAVCEGVLVSGLDIDAPMGRDPVRRTLQRVRADGRPALTRVRVVE
                     RFRAHTLVVAQLETGRTHQIRVHLSSIGHPLVGDRAYGARGRLPPGAQDELIAALRGF
                     SRQALHAAKLAFDHPVHGGRLQFEAPDPDDLVDLLDALRQDVRHHG"
     gene            1561..2559
                     /locus_tag="AC16QF_05880"
     CDS             1561..2559
                     /locus_tag="AC16QF_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR6448117.1"
                     /translation="MRKFTLMPFLLPLLLLCLASSGCSWMPWVGKDKDKAEEDLNATE
                     QQLYRDTQRALRAGNYDRAIQGLQQLEARFPFGRYAEQAQLEIIYAQYMSYDHDTARA
                     SADRFMRLHPQHANIDYAYYIKALAAFNKNRGLLDRIFATDLAKRDMTAARDAYADFA
                     QLLALYPDSQYAPDANLRMIYLRNLLAESEIHVADYYMRRGAYLAASNRARYVIETYS
                     KSQSVDDALAILVEANWRLGLEEAANDALRVLALNYPNYPAFDTDGNLTLASTVRNRD
                     RSWTNMVTFGLLDRPKVPPPITIKHPEGFEPPPVNPDAAPPPKKKGGIFSWLPFVG"
     gene            2654..4270
                     /locus_tag="AC16QF_05885"
     CDS             2654..4270
                     /locus_tag="AC16QF_05885"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF051540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6448118.1"
                     /translation="MTLQHEDIRREVEQRRFEVNANLLRIYNYYRVFVGLALLAVFMT
                     SLMGSRLGGLDPTAFLWSILAYIAINLTAAVAVQFLPRSIVQGQTISAALVVFDILTL
                     TWLTYLSGGVASGLGLLILVSVSTGSIMVTSRATAVLAATASIAVLFEEFYLSLTAPA
                     LHDDYFQAGVLGVFYFAFSLSIHHLARRIREDDLRAMTQAAELADLERLNRQIVQSMR
                     TGIIVVDADNRVRMMNQSATALLGLAGDEALARLPEPLEERLEDWRENSRLRTPPFQI
                     EPDTPEVRANFSAVRAGGDVTIFLEDTSELQQQAQQLKLAGLGRLSANIAHEIRNPLG
                     AISHAAQLLRESQNLDEGETRLTQIIHNHCQRMNGVIENVLEMSRRSPSAPVRLRLQE
                     LLEEFADSFREAVNDAIIDIDVVPEDTEIRVDRSQMSQVLTNLAGNGIRYSYEATGQP
                     FVRLEGGIDPRTDRPYLNVIDRGPGVPDDQVANLFEAFHTTERAGTGLGLYISKELCE
                     TNQARLSYSRHADGGSCFRIAFAHPDRITA"
     gene            4283..5665
                     /locus_tag="AC16QF_05890"
     CDS             4283..5665
                     /locus_tag="AC16QF_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR6448119.1"
                     /translation="MTGDKRALIVDDEPDIRELLEITLGRMSIETSAAQDLATARRYL
                     AEEEFDLCLTDMNLPDGNGIELVDLIAQERPGLPVAVITAFGSMDTAISALKAGAFDF
                     VQKPVDLDQLRALVDTALKIPEMAGSQAVDPARLTGQSDTMNRLREQIEKLARSQAPI
                     YISGESGSGKELAARLIHEQGPRRDAPFVPVNCGAIPSELMESEFFGHIKGSFTGAVA
                     DKEGLFQAADGGTLFLDEVADLPLSMQVKLLRAIQEKAVRAVGSAQEQAIDVRILSAT
                     HKDLADEVDAGHFRQDLYYRLNVIELPMPSLRDRADDIPELAEQILSRLAAEYGENKP
                     SISDAAMRSLGAYHFPGNVRELENILERAFTLCEDDCIEQADLQLSDVESVSARDVAE
                     EPTRSPSSPPTAVELGPSESLEDHLEAIERKLITDALESTRWNKTAAAKQLGITFRAL
                     RYRLKKLGLD"
     gene            5739..6296
                     /locus_tag="AC16QF_05895"
     CDS             5739..6296
                     /locus_tag="AC16QF_05895"
                     /inference="COORDINATES: protein motif:HMM:NF023444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HIR6448120.1"
                     /translation="MTRGVSLIEFLVCLIVASVLIGLAMPSLGEFVSRQRAVAAVNQI
                     IGAVAAARHAAVKHRATTTLCAAEAGQCVGANRWHLGAMIFKDVNANGTREEDELIVA
                     QLPAMRRGERVYWRSFRNKPYLQFSSRGLTSWQNGSFQYCPADADARLSKAVIINAQG
                     RAAVSRDNDGDGIDESAAGEPLYCR"
     gene            complement(6293..6631)
                     /locus_tag="AC16QF_05900"
     CDS             complement(6293..6631)
                     /locus_tag="AC16QF_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448121.1"
                     /translation="MKVTTLLLPAALVGMLCFYPLTARADCVCACVDAEPFMVCTGFI
                     ESVTTSDECSSTLDCSIVEESEPEGPPGLVCRSRHVYDTNTGDNQLKHVCHPRDLAKG
                     GEWGKTRGRN"
     gene            complement(6838..7176)
                     /locus_tag="AC16QF_05905"
     CDS             complement(6838..7176)
                     /locus_tag="AC16QF_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448122.1"
                     /translation="MASMRFPSLLMGLFMLCAMMASVAASAQAVYPEKIKTGTIQQLD
                     FAAGSMIVDGYRYHTTPELRVEIAGSHGAFTMLEVGMKIRMSYLIISPSRRDLVEVVQ
                     IPDNVTLEEA"
     gene            complement(7189..12717)
                     /locus_tag="AC16QF_05910"
     CDS             complement(7189..12717)
                     /locus_tag="AC16QF_05910"
                     /inference="COORDINATES: protein motif:HMM:NF017388.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HIR6448123.1"
                     /translation="MVINAVVDPVTGVVTFTPIPNANGTQTWVFTATDTTGNVGEFTF
                     TATVTPVEDAPVILVPDLVVTVSEDTASSSTDLLSVFSDADVATNGDSLTFSIESSPG
                     DPVDAVSIVGSNLVITYVPELNGTETVDVRATDLAGNWAQLTVGIVVNSVNDVPVIVG
                     SVSDVLEAEDSPGSTVGFGGVFDDIDIDTNGDSLTLSVSAVSHGAIASASMSGDTLNI
                     TYLPDQNGNGTVTVRATDSAGASVEYEVNVTVNPINDDPVLVASIPDINVFEDAVIAP
                     INLYNYFNDVDFATNGEALNFTVASNSNPSLFSNISIADGVLTLTLAPDQNGFANISI
                     EAVDAAGVMIADTFRVNVASINDVPLPEDDAVTMSEDQAFITIAVMDNDYLAEEPTFI
                     LSAGAGGFSESAPTTVVDAFGDGVTAPNGTVIIDATGTSIEYQPKPDFYGTDYFTYVL
                     QDSEGDVSLPATVTVTVKPVNDLPIGVQELTYSMFENGSLTVTATDGVLVGAYDVDGK
                     LIDETGTEIGSPLSAVLTVLPSVGALSFDGATGSFVYTPPINFIGTDSFGYRLFDNEG
                     VSAPPDYLVRIVVNAAPPPAEPPNPGEVAITYNLASVPLDQTASVPPNVLVVMDDSGS
                     MDWNMSVAGADENGGFVLDNSRRARRASAESFVYLWDLRTNTYPPGSQFGRVLPTEQA
                     LEDNGDTRGNDYGAWRARSHLHNKMYYNPTVYYTPWIGQDVSNAELEDAVPSAIRLDP
                     VDPTLTFDMLAPHSYSANGVPMWDPQGGTTDVTVVDLYIPRYYTTDATPPLAESAAAT
                     LIEIRDGAGPLVEGEFPGSPGREDCAADDDNPLDCTYAQEIQNFANWFQYYRSREYVI
                     KSVLGRVFADVQDIRVGYETISATTSIPVADMNDLYTEGNKKALIDNVYKVDSFGGTP
                     LRQALGRAGQIFACRTGNYCPALPPPDGTCQQNFALLFSDGYWNGGAGVSSNADVDGG
                     GPFDGGVYADTRRATLADTAMYYYENDMFSDVNDEVPVSPLDVLGAPEGTFAGETMHQ
                     HMKTYAIAFGVTGTIEPSSVPANPTTPFGWPDPFAGPLEKIDDMLHAAKNGRGEFLSA
                     GNPQELQAAFEKAFLAFTQAASSASAAAFNSTSLRDGTFLFRGFYDLRDNTGELTATV
                     VNADGTLADAPSWRASEMLNPGNKLPTNRVLVTADSLTGDGIPFRHPSLAPEQQIMMS
                     VDQTNFMRGERTAELPLGYLRERPPADGLLGDIVNSSPVFIGEPRAINRDQEPYPIGS
                     LYSDFVEAKASRTPIVYVGANDGMLHGFEAQTGIERFGYVPNKLIDSSQRYRNALDDL
                     TSPFYLHKYYVDLTPRFNDAYVKASRGGIGKSWNTILLGGLGGGGKGYFALNVTDPDT
                     MFASEAAAANSVLWEFTDEDDTYPVDDAGVPLGGAVGAITDPYGQPVKDLGMALSLPN
                     VLMSNVSDGGTPAEKEWVAIFGNGPNSTAGIAKLFVLFMDKGLDGWDDAGDFVKINTG
                     FGVPIAPAQKAGFPNGLGDPTAVDIDLNGTVDWVYAGDRLGNLFRFDLTDPNPDNWKS
                     TRLFSATYFDGAVERIQPITARPLVVKHPTQPGFLVIFGTGSYVTRDDARNEEIQSIY
                     AIWDRGELNPPTAQSDSKSLRLVEQTITNVVDDAVTPAVTRRVMSRNLVDYVAEGAGP
                     GTYGWYIDFDMVRAASTLSGGQNPDIGGNAPPDVQYPGEKAVRRMIFRDGTVITTTIL
                     PATGETSCFGARPGSILLFDAATGGDPVDQLVDFNNDGVINDGDMVSVGGSGYAAGLL
                     FNAGDLDGTLVDLSTLGGAGEMDFLFVSGGSDTSSFRISDINDSRTGRLSWREIDDVN
                     "
BASE COUNT         2493 a         4090 c         3902 g         2347 t
ORIGIN      
        1 ccaacctcat aagcgtcggc tccgattccc ggaccaatcc agcccacgta tcccgtcgtc
       61 ctgccaggta gcgagcccac cacgctgggg atgatgccgg caaccaggcc gcgccaaccc
      121 gcgtggatca cggcaaccgt gctcgcatcg cgagcagcaa cgaccaccgg caggcaatcg
      181 gcggtaagga cggccagcgc aagcccggga acagacgtcc acgcggcatc ggccaccggc
      241 acccggtcgc agcttgccag ggtggcctgg acacaccctg ttccatggac ctgctcgagc
      301 cattgcacgt gcgcaagacc gagcgctttg agcagatcgc gacggtttcg ggccacatgg
      361 gccgggtcat cgtggacgtg gcaggcgaga ttgaaggctg catacttccc cttgctgaac
      421 ccgcccggcc gggtcgtggt taccgcggcg attcggtggc cgggcgcgtc gtccggccac
      481 ctggcccact cccaagcgtt cgcttcaacc atggtgccgc acgtcctgcc gcagggcatc
      541 gagcaaatcc accagatcgt cggggtccgg agcttcgaac tgcaacctcc cgccgtgaac
      601 ggggtggtcg aaggcgagct tcgcggcatg cagcgcctgg cgcgagaaac cgcggagcgc
      661 ggcgatcagt tcgtcctgag cacccggtgg cagacgtccc cgagccccgt aggctcggtc
      721 ccccaccagc ggatgcccga tactgctcag atgcactctg atctggtggg tgcgtccggt
      781 ttccagctgc gcgaccacca gggtgtgagc cctgaaacgc tcgaccaccc ggacccgggt
      841 gagcgcgggg cgaccatcgg cccgaacgcg ctggagcgtc ctgcgcacgg gatcgcgccc
      901 catcggggca tcgatatcca gcccgctgac cagcacccct tcacagacgg cgcgatagcc
      961 ccgctgaatc tgtctggcct gcagcgccct gacaagagcg gcgtgagctt cgagattggc
     1021 cgcaaccagg agcagcccgg acgtgtcctt gtccagacgg tgcacgatcc ccgcccgcgg
     1081 aaccagggcc agcgaggccc ggtgatggag cagcccgttg acgagcgtgc catcgggatt
     1141 gcccgctcca ggatgaacca ctagccccgc aggcttgttg acgacgagca ggtcggcgtc
     1201 ttcatagagg acgtcaaacg ggacgggctc cccagtgctc cagtcttccc ggggcgcgac
     1261 gacggcatcg agcacgagtg tttcgccccc tacgagcttc acttttggtt tgacccgggc
     1321 accgtccacc gtgagcgccc catcctgaat ccaggcggtc aacgcggcgc gggagaattc
     1381 cgggaacagc cgggccgccg ccttgtcgac gcgctctccc gccacgtcag gtggcaccgt
     1441 tactcgtttc tggatttcca gcggctccgc catcgccctc tgctctggtt gcaaccgtac
     1501 gctaaatcgt taaattagcg aaactttctg gctcacgcct gaaccaaagc tgcaggatcc
     1561 atgcgcaaat tcaccctcat gccctttctt ttgcctctcc tactgctttg ccttgcgtct
     1621 tccggctgct cctggatgcc ctgggtcggc aaggacaagg acaaagccga agaggacctg
     1681 aacgcgacgg agcagcagct ctaccgcgac acgcaacgcg cgctccgggc cggcaactac
     1741 gaccgggcga tccagggtct ccagcagctc gaggcccggt ttcctttcgg ccgctacgcg
     1801 gagcaggccc agctggagat catctacgct cagtacatgt cctacgatca cgacacggcc
     1861 cgcgcgtccg cggaccggtt catgcgcctc catcctcagc atgccaacat cgactacgcc
     1921 tactacataa aggctctggc cgccttcaac aagaaccggg gcctgctgga tcgcatcttc
     1981 gcgaccgatc ttgcaaaacg ggacatgacc gccgcccgcg acgcttacgc cgacttcgct
     2041 cagctgctgg ccctttaccc cgacagccag tacgcgcccg acgcgaacct gcggatgatc
     2101 tatctgcgga acctcctggc agagtcggag attcacgtgg ccgactacta catgcgcagg
     2161 ggcgcttatc tggcggccag caaccgggcg agatacgtca tcgagaccta ctccaagtcc
     2221 caatccgtag acgatgcgct ggccatactg gttgaggcca actggcgcct ggggctggag
     2281 gaggccgcca acgacgcact gcgggtattg gcgctcaact acccgaacta cccggcgttc
     2341 gacaccgacg gcaacctgac cctggcgagc accgtgcgca accgcgaccg atcctggacc
     2401 aacatggtca cgttcggtct gctcgaccgt cccaaggtcc cgccgccgat cacgatcaag
     2461 catcctgaag gctttgaacc gccacccgtc aatccggacg ccgccccgcc accgaagaag
     2521 aaaggcggca tattcagctg gcttcccttc gtcggttgag cagaaagctg ctcccggtct
     2581 cagcaaacgg ccggcggtca tctagactca ggagcacgtg ccgcaaatcg gcaggcagcg
     2641 cgcaggcgga cggatgacgc ttcagcacga ggatataagg cgggaagtcg agcagcgccg
     2701 gttcgaggtc aacgccaacc tgctccgcat ctacaactac taccgcgtct tcgtgggtct
     2761 cgccctgctc gcagtattca tgaccagcct gatgggcagc cgtctgggag gcctcgatcc
     2821 cacggccttc ctctggagca ttctcgctta catcgccatt aacctgaccg ccgccgtggc
     2881 ggtgcagttt cttccccgca gcatcgttca ggggcagacc atcagcgccg ctctcgtggt
     2941 cttcgatatc ctgacgctca cctggctcac ttacttgagc ggcggcgttg ccagcggcct
     3001 cggcctgctg attctcgttt ctgtctcgac gggctccatc atggtcacca gccgcgccac
     3061 cgcggtgctg gccgccacgg cgagcattgc cgtgctattt gaggagttct acctctcgct
     3121 tacggcaccc gcccttcacg acgactactt tcaggccggc gtcctgggcg tcttctactt
     3181 cgccttttcc ctttccattc atcatctcgc cagacgcatt cgagaggacg atcttcgagc
     3241 catgacccag gcagcggagc ttgcagacct ggaacgcctg aaccggcaga tcgttcagag
     3301 catgcgcacg ggaatcatcg tggtcgatgc agacaatcgt gtgcggatga tgaaccagtc
     3361 cgcaacagcg ctactgggcc tggcgggcga cgaggcgctg gcgcgtcttc ccgagccgct
     3421 ggaggaacgg ctcgaggact ggcgagagaa ttcgcgcctt cggacgcctc ccttccagat
     3481 cgagcccgat accccggaag tgcgtgccaa cttctctgct gtgcgggccg gcggggacgt
     3541 caccatattt ctcgaggaca ccagtgagct tcagcaacag gctcaacagc tcaagctcgc
     3601 cggtctgggt cgcctctccg caaacatcgc ccacgagatc cgcaatcccc taggcgccat
     3661 cagccatgcg gcccagttgc tgcgcgaatc gcagaacctg gacgaagggg agacccgcct
     3721 cacccagatc atccacaacc attgtcagcg gatgaacggc gtcatagaaa acgtgctaga
     3781 gatgtcacgt cgcagcccgt ccgctcccgt tagactgcgt ctgcaggagc ttttggaaga
     3841 attcgcggat tcgttccggg aagcggtcaa tgacgccatc atcgacatag acgtcgtgcc
     3901 ggaggatacg gagattcggg tggacaggag ccagatgagc caggtgctca ccaatctggc
     3961 cggcaacggc attcggtaca gctacgaggc gaccgggcaa cccttcgttc gtctggaggg
     4021 agggatcgat ccgcgaaccg accggccgta tctcaacgtc atcgaccgcg ggcccggcgt
     4081 tccagacgac caggtcgcca atctgttcga agcgttccac accacggagc gggcgggcac
     4141 cgggctcggt ctgtacatat ccaaggagct ctgcgagacc aaccaggcgc ggctgtccta
     4201 ctcgcgacat gccgacggag gcagctgctt tcggattgcc ttcgctcatc ccgatcgaat
     4261 tacggcataa ggagaggcca aagtgacggg cgacaaacgt gcactcatcg tcgacgacga
     4321 gccggacatt cgcgaacttc tcgagatcac cctgggcagg atgagcatag agacctccgc
     4381 cgcccaggac cttgctacgg cacgtcgtta cctcgcagaa gaagaattcg acctctgcct
     4441 cacggacatg aacctccccg atggcaacgg catcgagctg gtcgacctga tcgcccaaga
     4501 gaggccggga ttgcccgtgg cggtgatcac ggccttcggc agcatggaca cggcaatcag
     4561 cgccttgaaa gcaggcgcct tcgatttcgt gcagaaaccc gttgatctgg accagctccg
     4621 agcactggtg gacacggcgc tgaagatccc cgagatggcc ggatctcagg ccgtcgatcc
     4681 ggcaaggctg acggggcagt cggacacgat gaatcgactg cgcgagcaga tcgagaaact
     4741 cgcccgcagc caggctccga tctacataag cggcgagtcc ggttcgggta aggaactcgc
     4801 cgcccggctc attcacgagc agggacccag gagggatgcg ccgttcgtgc cggtcaactg
     4861 cggggccatc ccgagcgagc ttatggaaag cgagttcttc gggcacatca agggcagctt
     4921 cacgggcgcc gttgcagaca aagaggggct ctttcaggcc gccgacggcg gcacgctctt
     4981 cctcgacgag gtcgccgacc tgccattgtc catgcaggtg aagctgctcc gggcgataca
     5041 ggaaaaggct gtgcgcgccg ttgggtcggc ccaggaacag gccatcgacg tacgcattct
     5101 ctcggcaacc cacaaggatc tggccgacga agtagatgcc ggtcacttcc ggcaggatct
     5161 ctactaccgg ctaaacgtca tcgagttgcc aatgccgagc ctgcgcgatc gcgccgacga
     5221 cattcccgaa ctcgccgagc agatcctgtc gcgccttgcg gcagagtacg gcgaaaacaa
     5281 accttccatt agcgacgccg caatgaggtc actcggggca taccactttc ctggaaacgt
     5341 ccgtgagctc gagaacattc tcgaacgcgc cttcaccctc tgcgaggacg attgcatcga
     5401 gcaggccgat ctgcaactga gcgacgtgga gtcggtatcg gcccgggatg tcgcagaaga
     5461 gccgacacgt tcgccgtcta gcccgcccac ggccgtcgag ctagggccga gcgagtcact
     5521 cgaggaccac ctggaggcca tcgagcgcaa gctgataacg gatgccctgg aatccacccg
     5581 ctggaacaag actgccgcag cgaagcaact gggcatcact tttcgcgcgc tgcgctaccg
     5641 gctgaaaaag ctgggcctcg actgagctga tatcccacca caagaggcgg cgcgcgccgc
     5701 ttctccccgc gacgcgcgtc ggatgaactg acgcggaaat gacacgcggc gtttctctca
     5761 tcgaatttct ggtctgcctg atcgtcgcgt cggtcctgat cgggctcgcg atgccatcgc
     5821 tgggcgagtt cgtgtcccga cagcgcgccg tggctgccgt caaccagatc atcggcgcgg
     5881 tagctgctgc aagacatgcg gcggtcaagc atcgcgcgac caccaccctt tgcgccgccg
     5941 aggccggcca gtgcgtcggc gccaaccgct ggcacctcgg cgccatgatc ttcaaggacg
     6001 tcaacgcgaa cggaacgcgg gaggaagacg agctcattgt ggctcagctg cccgccatgc
     6061 gccgcggtga aagggtttac tggcgctcgt tccgaaacaa gccctatctc cagttctctt
     6121 cacgtggcct gaccagctgg cagaacggaa gttttcaata ctgcccggca gacgccgatg
     6181 cgcggctgtc gaaagcggtg atcatcaatg cacagggacg cgctgcggtg tccagggaca
     6241 acgatggtga cggcatcgac gaaagcgcgg ccggcgagcc gctgtattgc cgttagttac
     6301 gccctcgtgt tttcccccac tcgccgccct tggctaagtc gcggggatgg catacgtgct
     6361 tgagctgatt gtctccggtg ttggtgtcgt agacgtgccg gctccgacac acgaggcccg
     6421 gcgggccctc gggctcgctc tcctcgacga tggagcagtc cagcgtggac gagcactcgt
     6481 ccgaggtcgt caccgactcg atgaagcctg tgcacaccat gaacggttct gcgtcgacgc
     6541 acgcacacac acaatcggcg cgcgcggtga gcggatagaa acaaagcatc ccgacaagcg
     6601 ccgcgggcaa aagcagggtt gttaccttca ttgtctgact cctcaagccg tattcgacac
     6661 cccatggaat acaagacgct gagtcgtcac ggatggtttc agcgcaagtg tgacgagttg
     6721 ccgattttcg ggattcaccc cgcagcttta gcggcgcgga gctcgctcag ggaacgcttt
     6781 gagaacgcgg ctttcccggc ccaactgaac gtcgggccgg gagcccgtga aggcaggtca
     6841 ggcttcttcc agcgtgacgt tgtccggtat ctgcacgacc tcgaccaggt cgcgtcgact
     6901 cggcgagatg atcaggtagg acatgcgtat cttcataccc acttcgagca tggtgaacgc
     6961 cccgtggcta ccggcaatct ccacccgcag ctccggcgtg gtgtggtacc ggtaaccgtc
     7021 aacgatcatc gagcccgccg cgaagtccag ctgctgaatc gtcccggtct tgatcttctc
     7081 cggatagacc gcctgggccg acgccgctac ggaagccatc attgcgcaca acatgaacag
     7141 gcccatcagc aggctcggga atctcatgct agccatatcg gtttcctcct agttaacgtc
     7201 atcgatctcg cgccaggaca ggcgtcccgt gcggctgtcg ttgatatcgg atatgcggaa
     7261 cgacgaggtg tcgctgccgc cgctgacgaa caggaagtcc atctcccctg caccgccaag
     7321 agtagacaga tccaccaggg taccgtccag atctccggcg ttgaagagaa ggccggccgc
     7381 gtatccgctg ccgccaacgg agaccatgtc accgtcgttg atgacgccgt cgttgttgaa
     7441 gtccaccagc tggtcgaccg ggtcaccacc ggtggccgca tcgaacagca ggatggaccc
     7501 cggccgcgcg ccgaagcaag aggtctcgcc ggttgcgggt agaatcgtgg tcgtgatcac
     7561 cgttccgtct cggaagatca tcctgcggac ggctttctca ccggggtact gcacgtccgg
     7621 aggcgcgttg ccaccgatat caggattctg gcccccagag agcgtgcttg cagcccggac
     7681 catgtcgaag tcgatgtacc agccgtaagt acccggacca gcgccttcgg ccacatagtc
     7741 caccaggttg cgcgacatga cccttcgcgt aaccgctggc gtcaccgcat cgtctaccac
     7801 attggtgatc gtctgctcca cgagccgcaa ggatttgctg tcgctctgcg cggtcggcgg
     7861 attcagctca ccgcgatccc agatcgcata aatcgactga atctcttcgt tacgggcatc
     7921 atcccgggtc acgtagctgc cggttccgaa tatcaccagg aagccgggct gagtgggatg
     7981 cttcaccacc agcggccgcg ccgtgatcgg ctggatgcgt tcgacggccc cgtcgaagta
     8041 agttgccgag aacagccggg tggacttcca gttgtcgggg ttcggatcgg tgaggtcgaa
     8101 ccggaacagg tttccgagcc ggtcgcccgc gtaaacccag tccacggtac cgttcaggtc
     8161 gatgtcaacg gcggtaggat caccaagacc attgggaaag ccagccttct gagccggcgc
     8221 aatgggcaca ccgaagcccg tattgatctt tacgaagtcc ccggcatcat cccagccgtc
     8281 gaggccctta tccatgaaca gaacgaacag cttggcaata cccgccgtgg aattaggtcc
     8341 attgccgaat atggccaccc actccttctc ggccggcgtt ccaccatcgc tgacgttgct
     8401 catgagcaca ttgggaagag agagtgccat gccgaggtct ttaacgggct gaccataggg
     8461 atccgtgatc gctcccacgg cgccaccgag cggcacgccc gcgtcgtcca cgggataagt
     8521 atcgtcttcg tccgtgaact cccacagaac ggaatttgcc gctgctgcct cgctggcaaa
     8581 catggtgtca ggatcggtca cgttgagcgc aaagtagccc ttaccgcctc caccgaggcc
     8641 gccgagcagt atcgtgttcc acgacttgcc gatcccgccg cgcgaggctt tcacgtaggc
     8701 gtcgttaaac cgcggggtca ggtcgacgta gtacttgtgg aggtagaaag gtgaagtgag
     8761 gtcatccaga gcattgcgat atcgctggga gctgtcgatg agcttgtttg gtacgtagcc
     8821 gaaccgctcg atgcccgtct gggcttcgaa tccatgaagc atcccgtcat tcgcgccaac
     8881 gtagacgatg ggtgtcctgg acgccttggc ttccacgaag tccgagtaga gactcccgat
     8941 cgggtatggc tcctggtcgc gattgatggc acgcggctca ccgatgaaca ccgggctgga
     9001 gttgacgatg tcaccgagca gaccatctgc ggggggccgc tctctcagat atcctagagg
     9061 cagttctgcc gtccgctccc cacgcataaa gttggtctgg tctacagaca tcatgatctg
     9121 ctgctccggc gccagagagg ggtgcctgaa cggaatgccg tcgccagtca aggagtcggc
     9181 agtcaccagc acccggttcg taggcagctt gttgccggga ttcaacatct ccgacgcccg
     9241 ccaggaaggc gcatcagcca gggttccgtc tgcgttgacc acggtggcgg tgagctcacc
     9301 cgtgttgtcc cgcaggtcgt agaatccgcg gaacaggaag gtgccgtcgc gcagggacgt
     9361 ggagttgaag gccgcagccg aagcggacga ggccgcctgg gtgaaggcga ggaacgcctt
     9421 ctcgaaagcc gcctggagct cctgaggatt gccggcactg agaaactcgc cccggccgtt
     9481 cttcgcggcg tgaagcatgt cgtcgatctt ctccagaggt ccggcgaagg gatcgggcca
     9541 accgaaggga gtggtcggat tcgctggcac gctcgaaggc tcgatggtgc cggtcacgcc
     9601 gaacgcgatg gcatacgtct tcatgtgctg gtgcatggtc tcgcctgcaa aagtgccctc
     9661 cggcgctcca agaacatcga gcggcgacac cggcacctcg tcattcacat ccgagaacat
     9721 gtcgttctcg taatagtaca tggcggtatc ggccagggtg gctctcctgg tgtcggcgta
     9781 aacgccgccg tcgaacggcc cgccgccgtc cacgtcggcg ttgctggaaa caccggcgcc
     9841 gccattccag taaccgtcgg aaaacagcag cgcgaagttc tgctggcatg tgccatcggg
     9901 cggcggcagc gcagggcagt agttgccagt ccgacaagcg aagatctggc cggcacgtcc
     9961 aagggcctgc cggagcggcg tgccgccgaa cgagtcgacc ttgtacacgt tgtcgatcaa
    10021 cgctttcttg ttaccttcgg tgtagagatc gttcatgtcg gccacaggaa tggatgtcgt
    10081 cgcgctgatg gtctcgtatc cgacccggat gtcctgcacg tcggcaaata cccgaccgag
    10141 cacgctcttg atcacgtact ctcgagaccg gtaatactgg aaccagttgg cgaaattctg
    10201 aatctcctgc gcgtaggtgc agtcgagcgg gttgtcatcg tctgccgcgc agtcctctcg
    10261 acccgggcta ccaggaaact cgccttcgac caagggaccg gccccgtcgc gaatctcgat
    10321 cagggtcgcc gcggcgctct ccgccagagg tggcgtcgca tcggtggtgt agtacctggg
    10381 aatgtagaga tcgacaacgg tgacgtcagt ggtgccaccc tgtggatccc acatgggaac
    10441 tccattggcc gagtagctgt gcggcgcgag catatcgaat gtaagggttg gatctaccgg
    10501 atcgagacgg atcgcggacg ggacagcatc ttctagttcc gcattggaaa cgtcctggcc
    10561 gatccacggc gtgtagtaga cggtcgggtt gtaatacatc ttgttgtgca ggtgcgagcg
    10621 tgctctccac gccccgtagt cgttgccccg tgtgtcgccg ttgtcttcca gcgcctgctc
    10681 cgtgggaagc acccgaccaa actggctgcc cggagggtag gtattggtcc gcagatccca
    10741 gaggtacacg aagctttccg cgctcgcccg acgggcgcgg cgcgagttgt cgagaacgaa
    10801 gccgccattc tcatcggcgc ctgccacgct catgttccag tccatgctgc ccgaatcgtc
    10861 catgaccacc agaacgttgg ggggcacgct cgcagtctga tcgagcggga cgctggcaag
    10921 gttgtaggtg atcgccacct cccctggatt cggcggctcg gcaggcggcg gcgccgcatt
    10981 gaccacgata cggaccaaat aatccggcgg cgcgctgacg ccctcgttgt cgaacaaccg
    11041 ataaccgaac gagtccgtgc caatgaagtt gatgggcggc gtgtagacga atgacccggt
    11101 tgcgccatca aaagaaagcg ctccgacgga gggcaggacg gtcagaaccg cgctgagtgg
    11161 cgaacctatc tcggtgccgg tctcatcgat cagcttgccg tccacgtcgt aagccccgac
    11221 gagcacgcca tcggttgccg taaccgtcag ggagccgttc tcgaacatcg agtaagtcag
    11281 ctcctggacg ccaatgggca ggtcgttgac gggcttcacc gtcaccgtaa cggtggcggg
    11341 cagagatacg tctccctccg aatcctgaag cacgtaggtg aagtagtcgg tgccatagaa
    11401 atcgggcttc ggctggtact cgattgacgt accggtcgca tcaatgatga cggtcccgtt
    11461 gggcgccgtg acgccgtcac cgaacgcatc gaccaccgtg gtcggcgcgc tttcactaaa
    11521 gccgcccgca ccggcagaca ggatgaacgt gggctcctcg gcaaggtaat cgttgtccat
    11581 gacggcaatg gtgatgaagg cctgatcttc ggacatggtc accgcgtcgt cttctggcaa
    11641 cggaacgtcg ttgatggaag cgacattcac cctgaacgtg tcggcgatca tgacgcccgc
    11701 ggcatcgacg gcctcgatgc tgatattggc aaacccgttc tgatcgggtg ctagggtgag
    11761 tgtgagcacg ccgtcggcga tcgagatgtt gctgaagagc gaagggttgc tgttgctggc
    11821 aaccgtgaag ttcagagctt caccgttggt agcaaaatcg acgtcgttga aataattgta
    11881 gaggttgata ggcgcgatca ccgcgtcctc gaagacgttt atatccggga tggacgccac
    11941 cagcaccggg tcgtcattga tcggattgac agtgacgttg acctcgtact cgacggatgc
    12001 tcctgccgag tcggtggcgc gcacggtcac cgtcccgttt ccattctgat ccgggagata
    12061 ggtgatgttc agggtgtcgc ccgacatgga cgcactggca atggcgccgt gggagacggc
    12121 cgacacgctc agggtcaggg agtccccgtt ggtgtcgatg tcgatatcgt caaacacgcc
    12181 gccgaacccg accgtggatc cgggggaatc ctcggcctcc agcacgtcgg aaaccgaccc
    12241 cacgatgacg ggaacgtcat ttaccgaatt gacgacgatt ccgacggtca gctgagccca
    12301 attgccagct aagtccgttg cccgaacgtc taccgtctcc gtgccgttga gctcgggaac
    12361 gtacgtgatg accagattcg agccgacgat gcttaccgcg tcgaccgggt ctcccgggga
    12421 actctcgatg gagaacgtca gcgagtcgcc gtttgtcgcg acgtccgcgt cgctgaacac
    12481 gctcaggaga tcggtcgacg agctcgccgt atcctcgctg accgtcacga ccagatccgg
    12541 caccagtatg acgggcgcat cttccaccgg cgtcaccgtc gctgtaaacg taaactcacc
    12601 gacgttgccg gtagtgtcgg tggccgtgaa cacccaggtc tgggtgccgt tcgcattggg
    12661 gatcggggtg aaggtaacca cgccggtgac aggatcgacc accgcgttaa tcaccacatc
    12721 gccgctcgtg gaggcgagac cgtaggtcag gacgtcgcca ggctcgtccg catcggagaa
    12781 gacgttattc agatagccag caaatggggc cgaatcttcc gctgtcgtgt ag
//
LOCUS       DBNHLM010000128         6389 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_78399, whole genome shotgun sequence.
ACCESSION   DBNHLM010000128 DBNHLM010000000
VERSION     DBNHLM010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6389)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_78399"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="AC16QF_05915"
     CDS             <1..583
                     /locus_tag="AC16QF_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin reductase domain-containing protein"
                     /protein_id="HIR6448124.1"
                     /translation="NYLCDLKAGDEVRLTGPNGKRFLLPADPDAHDYLLLATGTGIAP
                     FRGMVLELLTRPQGPCRSRIDLVMGAPYATDLLYDDLFRRLADEHDNFRYHTAVSREG
                     ADGGRGLYVDDLLRQRLEEFRPLLGGDRTLIYVCGLTGMRISLYRLLVEENLADGYLT
                     VDASLHDVDPGRWSAVDVRRGIKHSDRCLIEVY"
     gene            complement(609..1370)
                     /locus_tag="AC16QF_05920"
     CDS             complement(609..1370)
                     /locus_tag="AC16QF_05920"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448125.1"
                     /translation="MELGLEGKRAIILGGSRGIGWYTARALAVEGCSVALCARDADGV
                     QAAVDALSEDPGGSHFGAAADLTDGDGTRAFVREAITALGGLDLLIHNASGFDRSGDE
                     EGWQRSFQVDVMAGVRAVEEALPALKESDAASITFIGSMASKFYFGRRSSYGPAKAAM
                     RAYANELAQSFGRDGIRANVISPGAVHFPGGSWDRVKEAQPDFYRNVEKAIPLGRLGT
                     GEEIARIVAFVASPAGIWINSAHIAADGGQVAAVD"
     gene            complement(1410..3581)
                     /locus_tag="AC16QF_05925"
     CDS             complement(1410..3581)
                     /locus_tag="AC16QF_05925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR6448126.1"
                     /translation="MSEVRTAICRFCHSFCGVNVTLDDGRIIKVLGDIENPMYHGYSC
                     IKGRQLAEQHYHLERLLAPHKRSNGSHGKVPSMQALDEIAERLGELIDAHGPRSVATY
                     TGTYSFPYPAGTAMATAFMDAIGSPMRFTSGSIDQPGKPMAIAFHGRWSAGPPAFADS
                     DVWMLVGANPVVSKWGGIPQYNPGKRLHDALKRGMKLIVIDPRETECAKRAHLHLQCK
                     PGEDPTILAGIMHVIINEGLHDAEFIDSDVTGFDALKDAVAEFTPDYVAQRAEIPKAQ
                     LIEAARTFAAASRGMTTAGTGPNMAPRGTLTEYLVLSLNTLCGRWLQAGEVMPNPFAL
                     LPERRGRAQPEPKPEPFGYGERLRVRDLTENVSGLSAAALADEILLEGEGQVKALFCV
                     GGNPMAAWPDQLKTYQAMKALELNVCLDIKMSATAKLAHFVLPPRLGLESPAISLPNE
                     NIWFYGFSTGFPEPYAMYQPQLVEPPAGSDLLEEWEVFYGVAQRMGLGLTFAGEGLDM
                     NRKPTTDELFEIMCKGSRIPLEEVKRYPHGHIFSDPSITVLPKEEGCEDRLDVGHELM
                     MRELAEVRAEPLSGHGGYAGERAFTHRLVSRRMNDVYNSSGRDIPRLVRNHRYNPAYM
                     NPDDIEALGLHDGDVVEIASDHAKILGIVEAAPDIRPGVVSMAHAFGDAPEHDEDVFY
                     IGSNTGRLTDVERNYDPITGMPRMSAIPVNVRRGDQSLASR"
     gene            3639..5681
                     /locus_tag="AC16QF_05930"
     CDS             3639..5681
                     /locus_tag="AC16QF_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR6448127.1"
                     /translation="MARLTHLPTDLDGGERVRGFAFFIAGLLTIWGASGCSAPEDARD
                     EAPIEGPTAAAPTETGGASDEAGEGAVLASTDEFQVGLGDLGDREALPGAALYADNCA
                     ACHDGSVPKAPLFTWLEMMSARTLLGAMNDGIMAAQAADLTADERVHIVEYLTRQRYV
                     AGAVDDLADAHCEAPRAAFDRDRPAPRVGWGHDTRRFVPGDVAGLSAADVPDLELAWA
                     FVFPDALRARSQPAIGYGAVFVGSQDGRVYALDLETGCVRWHYQAAAEVRTGIVVAEI
                     HGRPMAFFGDILAWVHAVDALTGEPVWKRKADEHPGATLSGTPALHEGVLYVPVSSLE
                     IIAAADPAYECCTFRGSVLAVNAANGSERWRSYSIPEPPTVAQKTSAGTNVYAPSGAP
                     IWASPAIDAKRNRLYVGTGENYSTPADGNSDAVLAIDLTTGERIWTRQMTEGDAWNVA
                     CMMADNPNCPPEDGPDFDLGSSQMIVDLADGRQVLVGGHKIGAAFAFDLDAVKGDDPE
                     PLWWTRVGRGSIQGGVHFGMAADGTTIYVPINDMNDTRNGDVLDPEAARPGVHAVNAA
                     DGKVLWSRVQPDRCGDDRPFCDPGISAATTAVPGVLFAGHLDGVIRAYERDTGNVLWS
                     FDSAREFTGVDGRVGRGGGMSGAGPAVGEGYVVVNSGYGLYFHEPGNVLLAFRPSR"
     gene            5757..>6389
                     /locus_tag="AC16QF_05935"
     CDS             5757..>6389
                     /locus_tag="AC16QF_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6448128.1"
                     /translation="MAHLAVEGDRRIYFEHYPGAREVTVVLSHGWGMACRVWDNTTAC
                     LQDAGVGVVAYDHRNCGRSDKDFRDVSISALGDDVVALCDHLGLGGVVVNGWSLGGVV
                     VTDAAAKLGDRLAGLILTAAASPRYTQADGFPYGGQPEDVTATVAALRADRVNFLRTL
                     YYEGAYAKDVGEAVKNWCWQISLQASPGADASLGALANVDYREAMTQIAAP"
BASE COUNT          946 a         2081 c         2230 g         1132 t
ORIGIN      
        1 caattacctc tgcgacctca aagccggcga cgaggtgcgc ctgacggggc ccaacggcaa
       61 gcgcttcctg ctgccggcgg atcctgacgc gcacgattac ctcctgctgg caactggcac
      121 gggcatcgcc ccctttcgcg ggatggtgct cgagctgctg acacgcccgc agggaccctg
      181 ccggtcgcgc atcgatctgg tgatgggcgc gccgtacgcc accgatctgc tctacgacga
      241 tttgtttcgc cgcttagcgg acgagcacga caatttccgc tatcacacgg cggtaagccg
      301 ggagggcgcc gatggcggtc ggggtctcta cgtggacgac ctgctgcggc agcgtctcga
      361 ggagttccgg cctctgctcg gcggggatcg cacccttatc tacgtgtgcg ggctgaccgg
      421 catgcgcatc agcctctacc gcttgctggt ggaggagaac ctggcagacg gctatctcac
      481 cgtggacgct tctctgcacg acgtcgatcc cggccgctgg tcagcggtgg acgttcgccg
      541 gggcatcaag cactcggatc gctgcctcat cgaggtctat tgacggcgga aggcgcatcc
      601 cacggggctc agtccacggc ggccacctga ccaccgtcgg cggcgatgtg ggccgagttg
      661 atccagatcc cggcggggct cgccacgaag gcgacgatcc gggcgatctc ctcgccggtg
      721 cctagccttc ccagcgggat ggccttctcc acgttgcggt agaagtccgg ttgtgcctcc
      781 tttacccggt cccacgagcc gccgggaaag tgcacggcgc cgggcgagat gacgttggcg
      841 cggatcccgt cccggccgaa gctctgggcc agctcgttgg cataggcgcg catggcggcc
      901 ttggcgggcc cgtaggacga gcgccggccg aaatagaact tgctggccat ggagccgatg
      961 aaggtgatgc tggcagcgtc gctttccttg agcgcgggca gtgcttcctc caccgcccgc
     1021 acgccggcca tcacgtccac ctgaaagctg cgctgccagc cctcctcatc gccgctgcgg
     1081 tcgaagccgc tggcgttgtg gatcagcagg tccagcccgc cgagggccgt gatggcttcg
     1141 cgcacaaagg cccgggtccc gtccccgtcg gtcaggtccg ccgccgcgcc gaagtgggat
     1201 ccgccaggat cttcggaaag ggcgtccacc gcggcctgca cgccgtcggc atcgcgcgcg
     1261 cagagggcca ccgagcagcc ctcgaccgcc agcgccctcg ccgtgtacca gccgatgccg
     1321 cggcttccgc ccagaatgat ggcgcgctta ccttcgagtc ccagttccat gcgttttcct
     1381 ttgttcgtgc ccttggtcgt gcggtcgcct cagcgggaag cgaggctctg gtcgccgcgc
     1441 cggacgttca ccggaatcgc gctcatgcgc ggcatgccgg tgatggggtc gtagttgcgc
     1501 tccacgtccg tgaggcggcc ggtgttcgag ccgatgtaga aaacgtcctc gtcgtgctcg
     1561 ggggcgtcgc cgaaggcgtg agccatggac accacgccgg ggcgtatgtc gggcgctgcc
     1621 tccacgatgc cgagaatctt cgcgtggtcc gaggcgatct caacgacgtc cccatcgtgc
     1681 agccccaggg cctcgatgtc gtcaggattc atgtacgccg ggttgtagcg gtggttgcgc
     1741 accagccgcg gaatgtcgcg cccggaggag ttgtacacgt cgttcatccg gcggctgacg
     1801 aggcggtggg tgaatgcccg ctcgccggcg tagcccccgt ggcccgacag gggttcggcc
     1861 cgcacctcgg cgagttcccg catcatcagc tcgtgtccca catccagacg gtcctcgcag
     1921 ccttcttcct ttggcagcac ggtgatggat ggatccgaga aaatgtggcc gtgggggtat
     1981 cgtttgacct cctcgagcgg tattcgggaa cctttgcaca tgatctcgaa cagctcgtcc
     2041 gtggtgggct tgcggttcat gtccagcccc tccccggcga aggtgagccc aagccccatg
     2101 cgctgggcga cgccgtagaa gacctcccac tcttccagca ggtcggagcc ggctggcggc
     2161 tcgacgagct ggggctggta catggcgtaa ggttcgggga acccggtgga gaagccgtag
     2221 aaccagatgt tctcattcgg caacgagatg gcgggcgatt ccaggccgag ccggggcggc
     2281 agcacgaagt gggcgagctt ggcggtggcc gacatcttga tgtccaggca gacgttgagc
     2341 tcgagtgcct tcatggcctg gtaggtcttc agctggtcgg gccaggccgc catagggtta
     2401 ccgccgacgc agaacagcgc cttcacctgg ccttcgcctt ccagcaggat ctcgtccgct
     2461 aaggccgctg ccgacaggcc gctgacgttc tcggtcagat cccgaaccct cagccgctcg
     2521 ccgtaaccga acggttcggg ttttggctca ggctgggcgc ggccacggcg ctcgggcaag
     2581 agcgcgaagg ggttgggcat gacctcgccg gcctgaagcc agcgcccgca cagcgtgttg
     2641 agggagagca ccagatactc ggtcagcgtc cctcggggcg ccatgttggg cccggtgccg
     2701 gccgtggtca tcccgcggct cgccgctgcg aaggtgcggg cggcctcgat cagctgcgcc
     2761 ttgggtatct cggcccgctg ggccacgtag tcgggcgtga attcggccac ggcatccttg
     2821 agcgcgtcga agccggtcac gtccgagtcg atgaactcgg cgtcgtgcag tccttcgttg
     2881 atgatcacgt gcatgatgcc ggcgagaatc gtggggtcct cgccgggctt gcactgcaga
     2941 tggaggtggg ctcgcttcgc gcattccgtc tctcgcgggt cgatgacgat gagcttcatg
     3001 ccgcgcttca acgcatcgtg cagacgcttg cccgggttgt actgggggat gccgccccac
     3061 ttggatacca ccgggttcgc acccaccagc atccagacgt cggagtccgc gaacgccggg
     3121 gggccggcgc tccagcggcc gtgaaaggcg atggccatgg gcttgccggg ctggtcgatg
     3181 gagccggagg tgaagcgcat gggcgagccg atggcatcca tgaacgcggt ggccatggcc
     3241 gtgccggcgg ggtagggaaa cgagtaggtg cccgtgtacg tggcgacgct tctgggccca
     3301 tgggcatcga tgagctcgcc gagccgctcg gcgatctcgt ccagcgcttg catcgacggg
     3361 acctttccgt gggatccgtt gctgcgcttg tgaggcgcca gcaaccgttc gagatggtag
     3421 tgctgctccg cgagctggcg ccccttgatg caggagtagc cgtgatacat ggggttctcg
     3481 atgtcgccga gcaccttgat gatccgtccg tcatccagcg tgacgttcac gccgcagaac
     3541 gagtggcaga acctgcagat ggctgtgcgc acttccgaca tgatgatttc tccccgattg
     3601 gcccggccca ttttgtcgct ttgcggttgg gccggcaaat ggctaggctc acacatctgc
     3661 caactgatct ggatgggggg gagcgggtgc gcgggtttgc ctttttcatt gcagggctgc
     3721 tgacgatctg gggtgcttcc gggtgctcgg cgccggagga tgcccgggat gaggcgccca
     3781 tcgagggtcc gaccgcggcg gcgccgactg aaaccggggg tgcctcggac gaagccggcg
     3841 agggggccgt tctggcgtct accgacgagt tccaggtggg gctcggcgat ctgggtgacc
     3901 gcgaggcgct gccgggcgcg gccctttacg ccgacaactg cgcggcctgt cacgacggca
     3961 gcgtgcccaa ggcgcccctg ttcacctggc tggagatgat gtccgcgcgc acgctgctcg
     4021 gagcgatgaa cgacgggatc atggctgccc aggccgctga tctgacggcc gacgagcgcg
     4081 tccacatcgt cgagtacctg acccgtcagc gctacgtggc gggtgctgtc gacgatcttg
     4141 cggacgccca ctgtgaagcg cctcgcgccg ccttcgaccg tgatcgtccg gcgccaaggg
     4201 ttggctgggg gcacgacacg cgtcgcttcg ttcccggtga cgtcgccggc ctgagcgcgg
     4261 ccgacgtgcc cgatctggag ctcgcctggg ccttcgtctt tcccgacgcg ctgcgcgcgc
     4321 gttcccagcc ggcaatcggc tacggcgccg tcttcgtcgg cagccaggac ggccgggttt
     4381 acgccctgga tctggaaacg ggctgcgtgc gctggcacta ccaggcggcg gccgaggtcc
     4441 gaacggggat cgtcgtcgcg gagattcacg gtcggcccat ggccttcttc ggcgacatcc
     4501 tcgcgtgggt tcacgcggtg gatgcgctca cgggcgagcc ggtctggaaa cgcaaggcgg
     4561 acgagcaccc cggagccacg ctgtcgggta cccctgcgct gcacgagggc gtcctgtacg
     4621 ttcccgtctc ctcgctggag atcatcgcgg ccgccgaccc cgcctacgag tgctgcacgt
     4681 tccgtggcag cgtgctggcc gtgaacgcgg ccaacggctc cgagcgctgg cgcagctact
     4741 ccatccccga gcccccgacc gtcgcgcaga agacgtctgc gggcaccaac gtctacgccc
     4801 cttcgggcgc gcccatctgg gccagtccgg ccatcgacgc caagcgcaac cggctctacg
     4861 tgggcaccgg cgagaactat tccaccccgg cggacggcaa cagcgatgcc gtgctcgcca
     4921 tcgatctcac caccggtgag cgcatctgga cgcggcagat gaccgaaggc gatgcctgga
     4981 acgtggcctg catgatggcc gacaacccga actgcccgcc ggaggacggc ccggacttcg
     5041 atctgggctc cagccagatg atcgtcgacc tggctgacgg ccggcaggtg ctggtcgggg
     5101 gccacaagat cggcgccgcc ttcgccttcg acctggacgc ggtgaagggt gacgatccgg
     5161 agccgctgtg gtggacgcgg gtggggcggg gcagcattca gggcggcgtg cacttcggca
     5221 tggccgctga cggcaccacc atctacgtgc ccatcaacga tatgaacgac acccgcaacg
     5281 gcgacgtcct tgatcccgag gcggcccggc ccggcgtcca cgcggtcaac gcggcggacg
     5341 ggaaggtgct gtggagccgg gtgcagcccg accgctgcgg ggacgatcgg ccgttctgcg
     5401 acccgggcat ctcggcggcc accacggcgg tcccgggcgt cctcttcgcg ggccatctcg
     5461 acggcgtcat tcgtgcctac gagcgggaca ccgggaacgt cttgtggagc ttcgattccg
     5521 cccgggagtt caccggggtg gacgggcggg ttggtcgcgg cgggggcatg agcggggcag
     5581 ggcctgcggt gggcgagggc tacgtggtgg tgaattcggg ctatggcctt tacttccatg
     5641 agcccggcaa cgtgctgctg gcctttcgac cgtcccgctg atcgggcagc cccggttgcg
     5701 ctgaatgccg aggatcctta ggctcgatga gccttggttt tttgatgggg gaagtcgtgg
     5761 cacatctggc cgtagaaggc gatagacgaa tctatttcga gcactatccg ggtgcccgcg
     5821 aggtgacggt ggtgctctct cacggatggg gaatggcctg ccgggtatgg gacaacacca
     5881 cggcgtgcct tcaggacgcc ggcgtcggcg tggttgccta cgaccatcgc aactgcggac
     5941 gctctgacaa ggacttccgc gacgtctcca tctcggcatt aggcgacgac gtggtggcgc
     6001 tctgcgatca tctcgggctg ggtggggtgg tcgtcaacgg ctggtccctt ggcggggtgg
     6061 tggtcaccga cgccgcagcc aagctcggtg atcggctggc tggactgatc ctcacggcgg
     6121 ccgcctcgcc tcgctacacc caggccgacg gttttcccta tggcggtcag ccggaggacg
     6181 tgaccgccac ggtggccgcc ctgcgcgccg accgggtcaa cttcctgcgt acgctctact
     6241 acgaaggcgc gtacgccaag gacgtgggcg aggcggtcaa gaactggtgc tggcagatct
     6301 ccctgcaggc gagcccgggg gcagatgcgt ccctcggcgc gctggccaat gtcgattacc
     6361 gtgaggccat gactcagatt gcggctccg
//
LOCUS       DBNHLM010000129         2860 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1155100, whole genome shotgun sequence.
ACCESSION   DBNHLM010000129 DBNHLM010000000
VERSION     DBNHLM010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1155100"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="AC16QF_05940"
     CDS             <1..189
                     /locus_tag="AC16QF_05940"
                     /inference="COORDINATES: protein motif:HMM:NF028441.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HIR6448129.1"
                     /translation="ELLKTLTLGDYRQYEGRFWRPHELIMDNYRTGKKTLLRYERYEF
                     GTGLTERDFSQGTLKRVR"
     gene            199..1446
                     /locus_tag="AC16QF_05945"
     CDS             199..1446
                     /locus_tag="AC16QF_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448130.1"
                     /translation="MDSNDRTIPVGLWKHLITCASVLGSCAAGTIAGAAGEWELSGSV
                     AAELRIFVDSPAFVEQNDATFSPSASFEPEIVYEWNDGRDRLTAVPFLRLDAHDNNRT
                     HADLREANWLRVDDGWDLVIGIDKVFWGVTESRHLVDIVNQDDRVEDIDGEDKLGQPM
                     LNVNLYRDWGRLGIFILPGFRERTFPAKDTRLRGPLPIADTATYDSGAEEWRTDLALR
                     WALAIGAWDIGLAHFHGTSREPRFIPRVGVGGGEQLVPHYDQIDQTSIDLQLTTGAWL
                     WKLEALTRSGHGHRFAAVVGGFEVTHYDMLGIGADLSLLAEYLYDGRDKDRAPPTALE
                     DDIFLGVRLALNDPQDSSALLGTVVDRRLGAAIVFVEAERRLGNSWRIELESRLFFDV
                     PDDDPLAGIRNDSFVTLRLSRFF"
     gene            complement(1490..2461)
                     /locus_tag="AC16QF_05950"
     CDS             complement(1490..2461)
                     /locus_tag="AC16QF_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HIR6448131.1"
                     /translation="MRARELKQLLSMATRLTPHQRGQLQARLASDLALDEALAMIEAS
                     LPEQPACPSCQHERTVRNGQANGLQRYKCRECGTTFNALTGTPLARLRRRDLWLQQAR
                     ALDEGLSVRKAAQRMGVHRTTAFRWRHRFVMVARGVKAKQLHGVAEVDETYVLRSFKG
                     QPRRLAELSGRQARRPRRRGGHARKRGLSDEQVPILVLRDRAGQTTDEVLSNASRRCV
                     APVIRATLAADTVLCTDGSNMLANVARDLELEHQAVNTSRGERIRGAWHIQNVNAYHG
                     RWKEWMRRFKGVATSYLPSYLGWFRALDRNAQTGAKPSDLLTLAIRA"
BASE COUNT          530 a          892 c          884 g          554 t
ORIGIN      
        1 gagctgctga agactctgac gctcggcgac tatcggcagt acgagggacg gttctggcgt
       61 ccgcacgagc tgatcatgga caactaccgt accgggaaga agaccctctt acgctacgag
      121 cgttacgagt tcgggaccgg gttgaccgag cgggatttca gccagggcac actcaagcgc
      181 gtacggtgac actcggccat ggactcgaat gatcgaacga taccggttgg cttgtggaaa
      241 catctcatca cctgcgcgtc ggtactcgga tcctgtgccg ccggcaccat agcaggagcg
      301 gccggggaat gggagctctc gggatcggtc gcggctgagt tacgcatttt tgtcgattca
      361 ccggcgttcg tggagcagaa cgacgcgacg ttctctccct ctgcgtcctt cgagccagag
      421 atcgtctatg agtggaacga tggtcgcgat cggctgaccg cggtcccttt cttacgtctc
      481 gacgcccacg acaacaacag aacccatgcc gatctacgtg aggccaactg gctacgcgtc
      541 gacgacgggt gggacctggt cattgggatc gacaaggtgt tctggggtgt cacagagtcc
      601 cgacacctgg tcgacatcgt caaccaggac gaccgcgtgg aggacatcga cggcgaggac
      661 aagctcggcc agcccatgct caatgtcaac ctctaccgtg actggggtcg cctcgggatc
      721 ttcatactgc ccggtttcag agagagaacc tttccggcaa aagacacccg cctgcgaggt
      781 ccgctgccga ttgcggatac cgcaacgtac gattcggggg ccgaagaatg gcgcacagat
      841 ctggctttgc ggtgggctct tgcgatcggg gcgtgggata ttggccttgc ccattttcac
      901 ggcaccagcc gcgagccgcg attcatacca cgagtcggtg ttggcggcgg tgagcagctc
      961 gttcctcact atgatcagat cgatcagacc agcatcgact tgcagctcac gacgggggcc
     1021 tggctctgga agctcgaagc gcttacgagg agcggccatg gccatcggtt cgccgccgtg
     1081 gtcggtggat tcgaggtcac ccattacgac atgctcggca tcggcgccga tctgagtctg
     1141 ctggccgagt acctctacga cggccgtgat aaggacaggg cgccaccgac ggcgttggaa
     1201 gacgacatct ttctgggcgt gcggcttgcc ctgaacgacc cgcaagacag cagcgccctc
     1261 ttaggcacgg tggtcgaccg ccgcttggga gccgcgatcg ttttcgtgga agccgaacgg
     1321 cgtttgggta atagctggcg gatcgagctt gagagcagat tgttcttcga cgtaccggat
     1381 gacgacccgc tcgcgggcat acgaaacgac agtttcgtca ccttgcgttt gagccggttc
     1441 ttctgatcgg gttaagaatt ggctctgttc gcgttggcta ggggcgagat catgctcgga
     1501 tcgccaacgt gagcaggtcc gaaggcttag cgccggtctg cgcgttccgg tccagcgcgc
     1561 ggaaccagcc cagataagac ggcagatagg acgtcgccac gcctttgaac cgccgcatcc
     1621 actccttcca gcgcccgtga tacgcattga cgttctggat atgccaagcg ccgcggatac
     1681 gctcgccacg ggaggtgttg accgcctgat gctcgagttc gaggtctcgc gcgacgttcg
     1741 ccagcatgtt gctgccgtcg gtgcatagca ccgtgtcggc ggccagcgtg gctcggatca
     1801 ccggggcgac acaccgcctg ctggcgttgc tcagcacctc gtccgtcgtc tggccagccc
     1861 gatcgcgcag caccaggatc ggcacctgct catcggaaag accgcgtttg cgggcatgac
     1921 cacctcgtcg acgcggccga cgagcttgac gcccactcaa ctcggccaag cgtcgcggct
     1981 gccctttgaa ggaacgtagg acgtaggtct catcgacctc ggccacgcca tggagctgtt
     2041 ttgccttgac tcctcgggcg accatcacga agcgatggcg ccaacgaaag gccgtggtgc
     2101 ggtgcacacc catccgctgc gcagccttgc gtacgctcaa cccctcgtcg agggctcgag
     2161 cctgctgcaa ccaaaggtca cgccggcgca gccgcgccaa gggcgtgccc gtcagggcgt
     2221 tgaatgtcgt cccgcactcg cggcacttgt agcgctgcag cccgttggct tgaccgttac
     2281 gcacggtgcg ctcgtgctgg caggaaggac aggcgggttg ctcgggaagc gaggcctcga
     2341 tcatggccag agcctcgtcc agagccaggt cggaagccag ccgggcctgc aactggcctc
     2401 gctggtgcgg tgtcagtcga gtcgccatcg acagcaactg cttgagttct cttgctcgca
     2461 tcgcggttct cccctcggcg tacaaccgcc aggccgtgac gccagtctgc cgagggatat
     2521 ggctcaggac gtgagcccat cactagcgaa cgcgaacaga gcctttggaa ttcgacggct
     2581 gcgcagcccc gccgcagcag tcctgatacc gggcgtcaac cgctgaggtg gttaacaggc
     2641 actgtcacct gacggccatc gaatcgcact tacttgccca ggatcatggt ccccagcagg
     2701 tgagtcactg atcctcttcc cgctactgag gagccggaca tgacaaaact cgaccagatc
     2761 aagcgtgacc ccagcctttc cacgatgttc agcgttgtgc tggccacctt catcctcttc
     2821 aattccctat tcatttacct gttcatcggc cccttggcag
//
LOCUS       DBNHLM010000130        28562 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_437233, whole genome shotgun sequence.
ACCESSION   DBNHLM010000130 DBNHLM010000000
VERSION     DBNHLM010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 28562)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 28562)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28562
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_437233"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="AC16QF_05955"
     CDS             complement(<1..890)
                     /locus_tag="AC16QF_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6448132.1"
                     /translation="MSTPRLLEVEDLKVEFDTYGGVVQAVRGVSFGVDEGETLAIVGE
                     SGCGKSVTVQSIMGLTPMPPGRITSGSARLRGTDIIGRKEIGGEDIRGGQIGMIFQDP
                     MTSLNPTMNIGSQIAETLEVHRGYSKKQAFQRAVELLDMSRIPEAASRARQYPFEFSG
                     GMLQRAMIAMAIACEPAILIADEPTTALDVTIQAQILDLMLDLQRETGMAIILITHDL
                     GVVARMADEVAVMYAGQIAEQGTVDDVFYRSSHPYTLGLRAAMPNNDPARQHELKPID
                     GSPPDLFHPPKGCAYAARCP"
     gene            complement(887..2254)
                     /locus_tag="AC16QF_05960"
     CDS             complement(887..2254)
                     /locus_tag="AC16QF_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6448133.1"
                     /translation="MAETALTARDFEPVHAERRIQTISRPSLSYWQDAWIRLKANRRA
                     LFSLYIVLGLLAFTIAGPFVWTVDPAAQDVDQISQPPGTDRRAAVVEPYQPWDGSSVL
                     PLTEGLRLAEPATNQAVRLMWDATPGAAGYVIYRNLFPVGPENALGLPLGESFSAEDT
                     KFEDLLDLRPRRYYYSVVSLGAAGNELAQETLMVNVDRVITVAEAEARGMGEGRQLAV
                     GDIVRLPLHPLGTDYLGRDLLARLMEGARVSLFIGICAPFLYVLFGVAYGSAAGFLGG
                     HFDQALMRFADFVIALPFLLFMILFKIAFGIGPGESGIMPMLAAMVLLSWPGTSRLVR
                     GQILQIREEGYISASRLLGAKTGYLVLRHMIPNTMGVILVTLTFAVPSAIFTEAFLSF
                     IGMGVAPPTPSWGSMCNEGIRTMLTTPHELIFPALFISVTVLAFNLLGDGLRDALDAR
                     MRSTE"
     gene            complement(2258..3190)
                     /locus_tag="AC16QF_05965"
     CDS             complement(2258..3190)
                     /locus_tag="AC16QF_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6448134.1"
                     /translation="MLNYTLKRLLQGIVTVWFIATATFIAMHSVPGDPLADEKAVTAE
                     IRANLEAKYGLDKPVFDQYLIYMGNIVKGDFGISFTQENREVNDIIREHFPVSATLGI
                     LAVVFAGLGGVLWGALTALYRNRLPDVIIMFLVILGISVPSFVIAAISQLSLVNLNDL
                     LGVSILPVAGWGSVWHMLVPSMVLGLGTMAYLTRLMRSSMLEIVNADYVRTAKSKGLP
                     PTRIFTRHQLRNAILPVITVLGPSIAAITTGGFVVELVFAIPGLGRYFVQAVQQLDYT
                     VIMGTTVFYGSFLVFMVIVVDLLYGLIDPRVRLE"
     gene            complement(3201..4895)
                     /locus_tag="AC16QF_05970"
     CDS             complement(3201..4895)
                     /locus_tag="AC16QF_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6448135.1"
                     /translation="MTTVAGSEDTFNRSAGRQLLLFAAISIGSIGLLMYALAWASSLT
                     GSGAGSEVAVDPETRTVTLFLREEPPQLNSSLATDMVSGMVLGHVMEGLLRYDEHNRL
                     VPGMAERWEIDESGAIFHLRSDAVWSDGAPVTAHDFVFAWQLALDPANASQYAFILYP
                     VKNGRAIAEAKLPVSQLGARAVDDRTLEVTFERPVAYFDKLVAFPTYYPIRRDFYEAT
                     KGRYGADAHTLLYNGPFRMTRWVHGAHVRLEKNPTYWDEERIRIEVLDFPYITSDANA
                     TLNLFKDNKVAYAGLLAENLNDAMEQRWHIKRFMDGSVFFIEFNFRDGRVTANTNLRK
                     AMQHAVDPAELVYKVTKLPGYIPGESLFPVWLRGVNGYFRQEYPAPKTIKDVVKAREY
                     LQAAKRELGMEQIPPLVLLSGDNPVSNIQSEYFQQVFKQTLGLDIRIDKQIFKQRLAK
                     MTSGDFDMVLAGWGPDYDDPLTFGDLFASWNLNNRGRYSNPELDRQVAIAEESLDPKT
                     RMDAFGEIQRILHDDVVILINYEPGTTYVVNPDLGGLVRRSVGPDPDFTNAYIVEG"
     gene            complement(4909..6408)
                     /locus_tag="AC16QF_05975"
     CDS             complement(4909..6408)
                     /locus_tag="AC16QF_05975"
                     /inference="COORDINATES: protein motif:HMM:NF017569.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR6448136.1"
                     /translation="MDRTLTNFIRALRNSEVRISTAETLDAFNTVELVGYRDRAALKR
                     SLALVLPKTADEKETFEQCFDAYFSFTDIQGSQGAAGEAERASDAETTAGGEEGGEGG
                     GAQPGEARPGDAAGSAKKKQKPGASADAEADEDEDLGAGAMSKPQSTLGQLLMANSKV
                     ELSVATAAAGEAVDVRDIEVFTQKGLYTRRIMEEMGLADLNREISELRQRPAVPDRRL
                     GQELTRRRDWLREQVRDYVEKQFLLHADASGKRLREELLRKVKLSNVEQRSFRLIQEI
                     VFRMAKKLAALHSRRKKVFKRGQINIPRTLRHNMAYDGAIFDLRWKSVKIDRPKVFAI
                     CDVSGSVANYARFMLMFLYSLEEVMPKVRSFAFSSDLAEVTELFERNAIEDAIAKTIR
                     DYSGGSTDYGQSLMDFKKLCLDEVDNRTTIIILGDARNNYGDPHTELLKELYDRCKRL
                     IWLNPEPRNAWGVGDSEMRRYAAYCHQVEECNSLMHLERIVGNLLRVVQ"
     gene            complement(6415..7365)
                     /locus_tag="AC16QF_05980"
     CDS             complement(6415..7365)
                     /locus_tag="AC16QF_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6448137.1"
                     /translation="MAEAAVNIEELTESVGSLRDGLESHRYICSDQIATAVYLAYHLH
                     KPILIEGPPGVGKTELAKTTAEMFALPLIRLQCYEGLDEAKAIYEWKYGKQLLYTQVL
                     KETLDEVLQGAKGFAESVTRLHEFGDIFFSEEFLEPRPLLKALKEEAGCILLIDEVDK
                     SDHEFESLLLEMLSDFQVSIPEIGTVKAVRQPPMVFLTSNNTREISDALKRRCLHLYI
                     PFPDVDLEQRIIHARVPEIPPELRRQLVQFIHELRDLDLKKVPAISETIDWARTLLLL
                     HTESLDPKLVRDTLNVILKFQEDIDNVDAEVTTLTSKALK"
     gene            complement(7490..8362)
                     /gene="tesB"
                     /locus_tag="AC16QF_05985"
     CDS             complement(7490..8362)
                     /gene="tesB"
                     /locus_tag="AC16QF_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HIR6448138.1"
                     /translation="MSQDQTVTDLIDLLDLEEIEKNHYRATSPSEGWQRVYGGQVLGQ
                     ALVAASRTVPEDRHAHSLHGYFLRGGDTTVPILYTVDRIRDGRSFTTRRVVAIQHGKA
                     IFNMAISFQIDEPGLSHQMPMPEVPDPEGLPDESELRKAWADKLPEPLEAAFDRERPI
                     EVRPIDPLDVFAPEKRPPQQMCWMKCRDRLPDDPGLHQCVLAYLSDWSLLDTATLPHG
                     VSFMQDNMQMASLDHAMWFHRPFRADEWLLYVQDSPSASGARGLNRGLIYNRAGELVA
                     SAAQEGLIRLHETD"
     gene            8466..8942
                     /locus_tag="AC16QF_05990"
     CDS             8466..8942
                     /locus_tag="AC16QF_05990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6448139.1"
                     /translation="MPSRRELIALTEGEIRDYLAEQKTLIIVSNGKDGYAHPVPMWFW
                     TDPDGCLYCTTFSKAQKVLNFRRDPKASLLVESGDEYTELKSVLIYAKAEVIEEFDAV
                     REALVNISTKGRGDVSEAERAQIRDAVGKTAEKRVLLKFTPERYVSWDHAKLGGRY"
     gene            complement(8953..9657)
                     /locus_tag="AC16QF_05995"
     CDS             complement(8953..9657)
                     /locus_tag="AC16QF_05995"
                     /inference="COORDINATES: protein motif:HMM:NF015605.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6448140.1"
                     /translation="MQGLPEVSIGALLVAFLPSLALVAVMRAWSLQAGQALIANIRML
                     VQLLAIGYVLVFVFETENPWIVAAVLVMMMGVAAWIALRPLQSRSARKYLQSLTAISI
                     CGLAMLALVSQLVIDLPRWFEPRFVVPLAGMIFANAMNTVSLAAERFESEQAREVEYA
                     EARTTALDAALIPQVNALLAVGLVSLPGMMTGQILSGVDPLIAVRYQIVVMCMIFGSS
                     GLAAVAYLFAQRPRGA"
     gene            complement(9685..10071)
                     /locus_tag="AC16QF_06000"
     CDS             complement(9685..10071)
                     /locus_tag="AC16QF_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HIR6448141.1"
                     /translation="MTTPLWCLLIVMLMPIVLAGVGGYFRNKQLGTLDNDDPREQIKS
                     LTGAGSRAYAAQQNAWEALALFTAAALTAHVLGADPERSAQLAVAFVVFRVLHGVCYI
                     GGWATLRSLMFMGSLVSLIWLFVLGA"
     gene            complement(10081..11442)
                     /gene="ahcY"
                     /locus_tag="AC16QF_06005"
     CDS             complement(10081..11442)
                     /gene="ahcY"
                     /locus_tag="AC16QF_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003414325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR6448142.1"
                     /translation="MTDYKVADMSLADFGRREINLAEAEMPALMALRNRYADQQPLAG
                     AKVIGCIHMTVQTAVLIETLVALGAEVRWSSCNIFSTQDHAAAAMAAAGIPVFAWKGE
                     TEEEYWWCIEQTILKDGKPWDANLLLDDGGDLTEVIHEKYPEMLDSIHGVSEETTTGV
                     HRLYEMIDRGELKVPAINVNDSVTKSKNDNRYGCRHSLNDAIKRGTDMLMAGRRALVI
                     GYGDVGKGSAQSLRQEGMIVRVVEIDPICAMQACMDGFEVVSPYLGGENTGRIENIDT
                     RLLHDTDLIVTCTGNADVCDASMLSTVKAGAVVCNIGHFDNEIDTAFMREHWRWEMVK
                     DQVHQVFRSDDRHDYLILLSEGRLVNLGNAMGHPSRVMDGSFANQVLAQMHLYEQRWA
                     DQPENQRAPITVEVLPKKLDEEVAELMVAGFGGVVTRLTAEQAKYIGVDESGPFKPES
                     YKY"
     gene            complement(11503..12459)
                     /locus_tag="AC16QF_06010"
     CDS             complement(11503..12459)
                     /locus_tag="AC16QF_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HIR6448143.1"
                     /translation="MGQTAGQQANLDEVVEVAKAVGDHLRASILRVLSDGSYSVTELC
                     HLFQIPQPALSHHLKVLLTANLVAKRREGNNVYYRRCTEPDDGLRQRLFETVDGTPLG
                     AALAERAAEIHAERSQRSNNFFAENAHRFADQQALICEAQVYVPAIVELLDHAGVDSG
                     PIIEVGPGGGQLLATLAGSFDDLIGIDSSSTMLAQAAERLDGLPNVKLRQKDFTELPA
                     IRRYRAVIAAMVVHHLASPQRFFRHASRILRHGGLLIVAELDRHEQEWVTDACGDLWL
                     GFEPGELEQWAAQAGLAPVESQYLAQKNGFQIQIHGYARNGT"
     gene            12568..13728
                     /locus_tag="AC16QF_06015"
     CDS             12568..13728
                     /locus_tag="AC16QF_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR6448144.1"
                     /translation="MSWSSPDLVSKLPDVQTTIFTVMSALAREEGALNLSQGFPDFDG
                     PPELLERVQYYLTHGHNQYAPMTGVAPLREATAGKIADLYGCQLDPEQEVTVTSGATE
                     ALFCAIAAVVNAGDEVIVFDPAYDSYAPVVKLQGGVTRHVPLHPPDYRVDWDRVADNL
                     SDRTRLIIINTPHNPTGTVWGEDDIEALRAVIDGRDLFLLGDEVYEHIIFDGRQHASL
                     CRYPDLFARSFVVSSFGKTYHTTGWKIGYCAAPAPLSAEFRRIHQFVTFTSITPVQLG
                     LADFMTRHPEHHLGLGAFYEAKRDLFCSLLEGSRFGLTKSAGTYFQLLDYGDYSNEAD
                     TDLAVRLTREAKIASIPLSVFYADDPGHRMLRFCFAKDDATLRAGAEILLGL"
     gene            13798..14151
                     /locus_tag="AC16QF_06020"
     CDS             13798..14151
                     /locus_tag="AC16QF_06020"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6448145.1"
                     /translation="MKRPLLALLVCCAFGCSESSNETPASTAAAGDGPAPAAHPGEET
                     YNRFCFSCHAAGIAGAPRTGDARAWASRVAKGEDQLLKSTIEGIAPGMPAMGLCRQCS
                     EDDLRAAIDYMIPDA"
     gene            14341..14772
                     /locus_tag="AC16QF_06025"
     CDS             14341..14772
                     /locus_tag="AC16QF_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448146.1"
                     /translation="MTRYLPGDIVARRKGLVMHKGVVTGDGRIFHNTPFRGEHVCTEA
                     EFRAGKRLYVQRQEFEARQRAVEHAERHGGSGYNLFTNNCEHTVYRATSGVGHSPQLK
                     SWIAGLGLATVAFAVTRHPGAAAAGYALGRRLAGRSADRRR"
     gene            complement(14774..15478)
                     /locus_tag="AC16QF_06030"
     CDS             complement(14774..15478)
                     /locus_tag="AC16QF_06030"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR6448147.1"
                     /translation="MTPAIDVLHRSADLLVLNKPANVSLLADRSGEPCYWDALRQALG
                     DRTPYLVHRLDKGTSGVLLVALTRSAQRDLTRAFQQRAVRKFYVAWVVGSMALTSTHT
                     IDLPLQRGRKSRFRVAGPREQIRQQGTRWVLPATDTAGHPAITRIRTLANGHDRSLML
                     LAPRTGRSHQLRVHLSWIGYPIVGDHLYGRPTDPMQRADRLQLHCHRLVVPGFGTYRA
                     APGEGWLGRPDERPED"
     gene            15544..16428
                     /gene="oxyR"
                     /locus_tag="AC16QF_06035"
     CDS             15544..16428
                     /gene="oxyR"
                     /locus_tag="AC16QF_06035"
                     /inference="COORDINATES: protein motif:HMM:NF008361.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HIR6448148.1"
                     /translation="MNLRDLKYLVAVAEQRHFGKAADACHVSQPTLSTQIKKLEEELG
                     VVLLERTNRQVMLTPVGERIVAQAQRVLREVDDLVHTAEQFRDPFGGEFRLGLIPTVA
                     PYLLPRILGPIRKSFPNLKIRLTEGQTAVVGERLRAGDLDAVILALPLEEENVVEAEL
                     YREPFYFAASKNHPKAGKREVALSDLDQEQVLLLEDGHCLRDQALEVCNSQNAVENTN
                     FRATSIETLRQMVAADVGITLMPELAIPQKHGAVRYIPFKGNGPHRRIGLCWRGSSSR
                     AALLHDMAGVLREVMAGG"
     gene            complement(16456..17037)
                     /locus_tag="AC16QF_06040"
     CDS             complement(16456..17037)
                     /locus_tag="AC16QF_06040"
                     /inference="COORDINATES: protein motif:HMM:NF023432.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HIR6448149.1"
                     /translation="MQKLNRTDLWSLEQYSEERPAFRAEVIAHKKARRVAIGPHATLC
                     FEDALTMKYQIQEMLRVEKIFEAAEIEEELTTYNPLIPDGANWKATFLIEYPDVEERH
                     RALATMGGIEDTIWVQIGDGPRIYAIANEDMPRSEAGKAAAVHFLRFELDGASVQAAK
                     EGAPILAGIDHASLKGAVTLDPARAESLRRDLD"
     gene            complement(17041..18366)
                     /locus_tag="AC16QF_06045"
     CDS             complement(17041..18366)
                     /locus_tag="AC16QF_06045"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HIR6448150.1"
                     /translation="MKEGSLEAPDRQPLDWQNPQFYDKASLYAELERVYDICHGCRRC
                     VSLCDSFPTLFDLVDESETLEVDGVDKADYMKVVDQCFLCDLCAETKCPYLPPHEWAV
                     DFPHLMLRAKAQKFREKDTKWRDRLITSTDPIFDTLSIPGVAQMANAAAGNSALRKLG
                     DKAVGIHPDAPLPNFSSRPLNKRLDTEIGATLTPKPCERTTGKVAIYITCYGDHNEPQ
                     VVEDLIAVLNHNGIPVKILKDAKCCGMPKLELGDLSKVASLKDKNLPLFLEAIEAGYD
                     IIAPIPSCVLMYKQELPLMFPEDADVEQVKKAFFDPFEYLVLRHKAGLIDTGFGKPLG
                     KVAYHVACHQRVQNIGMKTREFLSLIPDTEVTAIERCSGHDGTYAVKSETYEKAMKIA
                     RPVVNRVKQAEADTFGSDCPMAGRLIAHGMEDDGAAEHPITMVRRAYGV"
     gene            complement(18479..18895)
                     /locus_tag="AC16QF_06050"
     CDS             complement(18479..18895)
                     /locus_tag="AC16QF_06050"
                     /inference="COORDINATES: protein motif:HMM:NF014912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HIR6448151.1"
                     /translation="MDLKDSNTLQNLKDAFAGESQANRRYLYFAAKADVEGENDVAAV
                     FRSTAEGETGHAHGHLEYMETVGDPATGLPIGSTGNNLKAAIAGETHEYTDMYPGMAK
                     TARDEGFDEIADWFETLAKAERSHANRFQKALDAMG"
     gene            complement(19092..19691)
                     /locus_tag="AC16QF_06055"
     CDS             complement(19092..19691)
                     /locus_tag="AC16QF_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448152.1"
                     /translation="MAESSRDRVRRHFASVQLTLLSIVVALILENLLSVYWERPQPFE
                     SDALQWLFWLQAGLVLATALSMWSGFALSFNYLHLRQPTTLDIIAPFGLLITMNLAIG
                     AMHPALPAGFLLASGSGSLLAGFILFLDTRNSRRRGKSGPVSALAIQISIALLSIAGA
                     GAIAFEWLGIPGACAVLAVALFTQVTSIVGIARSWRLAT"
     gene            19747..20628
                     /locus_tag="AC16QF_06060"
     CDS             19747..20628
                     /locus_tag="AC16QF_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6448153.1"
                     /translation="MNIELWIIITVAAAFLQNVRSMLQKRLTQRLSVNGATYVRFLYA
                     LPIAWIYLAALASRQDLPLPNSEFLLYCLGGGVGQILATACLVGSFAQRNFAVGTALS
                     KTEVVQAAIFGLLVLGDPVGWRVMMGIAVSLVGVLLLSNRVRLQDIFQADRSVLLGLA
                     SGAAFAVAIVSFRGASLALPSGDYLIRAALTLAVAVSLQSLIMGAYLGRFETGELRRV
                     VRAWRPASLVGLTGMLASAGWLTAVTLESAALVRAVGQVELLFTFAASIWFFGERVGW
                     REAVGVALVVIGIWVLL"
     gene            complement(20625..21194)
                     /gene="idi"
                     /locus_tag="AC16QF_06065"
     CDS             complement(20625..21194)
                     /gene="idi"
                     /locus_tag="AC16QF_06065"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HIR6448154.1"
                     /translation="MSHPATKSEIVSSEEELLILVDGDDREVGFLEKSACHDGAGVLH
                     RAFSLFVLNSAGEVLIQQRASGKRLWPDYWSNSCCSHPRRGETMHEAARRRLHQELGM
                     TAPLEFLYKFEYQADYRGLGAEHELCSVYVGHSDATPVINTTEIGDWRWIARDDLKRE
                     LDTRPEQFTPWFLMEWERILRDHPAALHA"
     gene            21324..22211
                     /locus_tag="AC16QF_06070"
     CDS             21324..22211
                     /locus_tag="AC16QF_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6448155.1"
                     /translation="MSAYGHPREHRFQGSQVELAWYEWGEAPHPKVLLVHATGFHARC
                     WDQVVAHLPPGFHILAVDLRGHGRSTKRGPYVWSSFLADLEEFAAALSLAGAVGVGHS
                     LGGHCITQLAARRPGAFDRLVLVDPVILDPEAYARPRYADLQSAEDHPVARRRDHWAG
                     WEEMFERFSSRKPFSRWRPQVLEDYCRHGLEPRAEGGWRLACPPVVEASIYLGNKGSD
                     VYGMLHDVDVPVAVLRAPGRDPDDHDIMDFSKSPTWPELADRFPRGRDVFLPRLDHFI
                     PMQAPELVARFITDGDADP"
     gene            22208..22462
                     /locus_tag="AC16QF_06075"
     CDS             22208..22462
                     /locus_tag="AC16QF_06075"
                     /inference="COORDINATES: protein motif:HMM:NF016141.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR6448156.1"
                     /translation="MNFLSWLLLGLVVGTLAKLIMPGPDPGGFFVTIGIGMAGAFIGG
                     YGAHLLGIGDVAGFDLASLGLATAGAVLLLGTYRVVRGGF"
     gene            complement(22435..23688)
                     /locus_tag="AC16QF_06080"
     CDS             complement(22435..23688)
                     /locus_tag="AC16QF_06080"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6448157.1"
                     /translation="MSEIALPPRTAYPWYLTSSSLWMAGMSLQGFLFTWLLVGILERP
                     ADEAGLARSLAEFPPLAVLLLGGLLGDRFDNRAYLGVMHLLMAVPPLLIAAVYLNGAL
                     SYWWVVLFGVLMASIQALSDPARQAVLSRVSRMDLQRAVTLMMVVTSLVGLSGVYLGG
                     QLDTLGLPTVLAAQAALFAVGLLAVARLPRLPSISARGVRRPSFAEGLRAVWETALIR
                     NIIGLNFISSLFNAGAYIIGIPYIVKEVYAGDAAFLANVMIVFTIGSIGSNVLLLAFM
                     PLKHPGRLYLLLQLTRMFILGLLWLQPSLWLFYVLIVAWGLNMGVTTTLVRTTVQELA
                     PEHHRAQILSVLLLSFVVSSPISSILLGVLIAETEPLTAMLPGIAISLLIFLIGTTRS
                     GLWGYVSPATKTRAAGPQNPPRTTR"
     gene            23803..24660
                     /locus_tag="AC16QF_06085"
     CDS             23803..24660
                     /locus_tag="AC16QF_06085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6448158.1"
                     /translation="MNIGAVMAFNHVMSPQFMADAAQMVENAGFHSVWLPEHVLFFPD
                     YESRYPYTEDGRIPGDPEGVLDPFTALTWIAAATKRIRLGTGICLVPQRQPVYTAKMV
                     ADLDYLSGGRVDFGIGIGWLKEEFDNLQMDFRARARRCLEYVEVMQALWSPGLSSYEG
                     ETVTLKPCHFNPKPVQQPHPPIFFGGESDPALARVVSHGQGWYGFNLSPEGLAGRKAR
                     LAEIAAEAGRDMNDVRIFIGPSGKPVTPETVSEYEDLGVEQLIVPVIAGNLERLAQRI
                     DKARAMTGL"
     gene            24798..25064
                     /locus_tag="AC16QF_06090"
     CDS             24798..25064
                     /locus_tag="AC16QF_06090"
                     /inference="COORDINATES: protein motif:HMM:NF039725.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6316 family protein"
                     /protein_id="HIR6448159.1"
                     /translation="MNVGRRNEKPKTWFRSERVFMNDGSWFFRTREGVEIGPYETQSE
                     ATMEAGMLRELLRDRGHDGKGIAVIREFVRDSYAMGRPLTPKFD"
     gene            25129..25785
                     /locus_tag="AC16QF_06095"
     CDS             25129..25785
                     /locus_tag="AC16QF_06095"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6448160.1"
                     /translation="MPRLKQISRADAPEHIQRMYGFLFGDRDPVAEPGTDTGTPGNWW
                     TVFALVPDCFDHAVAGFQLYRSPKRELEPTLRELGQTRAGYARGSRFVFSQHCKSLRY
                     LGFSEEQIEAIPHWQAADCFDEVERLVLAFTDCLVLDGGRVPEGLFAALKTHLSDEEI
                     LELTYITCMYDMHATMCRALRLEYDDVEERVSEIAAPEDGGDILTAVDESATGGSAED
                     "
     gene            25790..26554
                     /locus_tag="AC16QF_06100"
     CDS             25790..26554
                     /locus_tag="AC16QF_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6448161.1"
                     /translation="MTIDHVVRGSGPEPVLVMHDWNGDRRNYDPALPYLDGTTFTYAF
                     VDLRGYGGSKHIAGEHSVAEIAADCLGVADALGWGEFHVVGHSMTGMATQRLALEAPE
                     RVRSAVAVCPVSAAGMAMDDQTRAFFESTTTDDEAFRGLIGMISGSLSGGWVDAKLRW
                     NREAAAPDVRLDYLAMFADTDFAESVEGLETPFLVMVGEHDAEGLDAAAMQKTYLAWH
                     PNAELVVIPNCGHYPMQECPPFFVSTLESFLRRRAG"
     gene            complement(26579..27442)
                     /locus_tag="AC16QF_06105"
     CDS             complement(26579..27442)
                     /locus_tag="AC16QF_06105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6448162.1"
                     /translation="MEFGAVFPHNEIGTDPGAIKAYAQGVEAMGATHLLIYDHVLGAD
                     PNRPGGWKGAYDKDVAFHEPFTTFAFIAAVTDKLEMITTVLILPQRQTVLVAKQAAEV
                     AILSNNRFRLGVGSGWNQVEYVGLNEDFHTRGRRQAEQVELMRKLWSEDSLTFDGAFH
                     TIDAASINPRPGASVPVWFGGSAAPLLKRCAELGDGWIPLMGANPKARECIDTIRAHR
                     EAAGLPWEGFGIQAQAQYAGGNPDRWASHAGKWQDMGCTHLAIATHNAGPTNADGHLR
                     RIEEYLQAVAA"
     gene            27546..>28562
                     /locus_tag="AC16QF_06110"
     CDS             27546..>28562
                     /locus_tag="AC16QF_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012361.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR6448163.1"
                     /translation="MADPAQSSSSFSFVDMEHQILDFWEQGRVFQRSLENTREKKPYI
                     FYDGPPFATGLPHHGHIVASTIKDIVPRYWTMKGRYVLRRFGWDCHGLPIEHEIDKQL
                     NMSAQQAVAEMGVAGYNQQCRAIVQRYVDEWRHTITRLGRWVDFDNDYKTMDAWYMES
                     VWWVVKQLWEQGLVYQGVKVMPVSTALGTPLSNFEANQNYMDVQDPAITVLFKLVDED
                     AYLSAWTTTPWTLPSNLAICVGPDIDYVRVRDPQLGADVYMAAQRVADYGSFEEVGRV
                     KGRELVGRRYEPLFPYFREAGEEGAFVVVMDDYVTTESGTGLVHQAPAFGEDDYRIAR
                     AAGID"
BASE COUNT         4825 a         8990 c         9527 g         5220 t
ORIGIN      
        1 taggggcagc gcgcggcgta ggcgcatccc tttggcgggt ggaagaggtc gggcgggctg
       61 ccgtcgatgg gcttcagctc gtgctggcgg gccgggtcgt tgttgggcat ggctgcccgg
      121 agccccaggg tgtaggggtg ggaggagcgg tagaagacgt cgtccacggt gccttgctcg
      181 gcgatctggc ccgcgtacat caccgccacc tcgtccgcca tccgcgccac cacccccaga
      241 tcgtgggtga tgaggatgat ggccatgccg gtctcgcgct gcaggtcgag catgagatcg
      301 agaatctgcg cctggatggt cacgtccagg gcggtggtgg gctcatcggc aatgaggatg
      361 gccggctcgc aggcgatggc catggcgatc atcgcccgct gcagcatgcc gccggagaac
      421 tcgaacgggt actggcgcgc ccggctggcc gcctcgggga tccggctcat gtccaggagc
      481 tccacggccc gctggaatgc ctgtttcttg gagtagcccc ggtgaacctc cagggtctcc
      541 gcaatctggg agccgatgtt catggtgggg ttcagggacg tcatggggtc ctggaagatc
      601 atgccgatct ggccgccgcg gatgtcctcg ccgccgatct ccttgcgccc gatgatgtcc
      661 gtgccgcgca gccgggcgct gcccgaggtg atgcggccgg gcggcatggg ggtcaggccc
      721 atgatgctct gcaccgtcac cgacttgccg cagccggact cgccgacgat ggcgagcgtc
      781 tcgccctcgt ccaccccgaa gctcaccccg cggacggcct gcaccacgcc gccgtaggtg
      841 tcgaactcca ccttcagatc ctccacctcc agaagccgcg gcgtgctcac tccgtggacc
      901 tcatgcgggc gtcaagggca tcgcgcaggc cgtctccgag caggttgaag gccagcaccg
      961 tcacgctgat gaagagcgcg gggaagatca gctcgtgggg cgtggtcagc atggtgcgga
     1021 tgccctcgtt gcacatgctg ccccaggaag gcgtcggggg cgcgacgccc atgccgatga
     1081 aggacaggaa cgcctcggtg aagatggcgc tgggcaccgc gaaggtcagc gtgaccagga
     1141 tgacgcccat ggtgttggga atcatgtggc gcagaaccag gtagccggtc ttcgcgccca
     1201 gcaggcgcga ggcgctgatg tagccctcct cccggatctg caggatctgc ccgcgcacca
     1261 ggcgcgacgt gcccggccag gagagcagga ccatggccgc cagcatgggc atgatgccgc
     1321 tctcgcccgg gccgatgccg aaggcgatct tgaacaggat catgaacagc aggaagggca
     1381 gggcgatgac gaagtcggcg aagcgcatga gcgcctgatc gaagtgcccg ccgagaaagc
     1441 ccgccgcgct gccgtaggcc accccgaaca gcacgtagag gaacggcgcg cagatgccga
     1501 tgaagaggga caccctcgcg ccctccatga gccgggccag gaggtcgcgg cccagatagt
     1561 ccgtgcccag aggatgcagg ggcagcctca cgatgtcgcc gacggccagc tggcggccct
     1621 cacccatgcc ccgggcctcg gcttccgcta cggtgatgac ccggtcgacg ttgaccatca
     1681 gcgtctcctg agccagctcg ttgccggcgg cgcccaggct gacgacgctg tagtagtagc
     1741 ggcgcgggcg aagatccagc agatcctcga acttcgtgtc ttcggcggag aagctctcgc
     1801 ccagcggcag ccccagggca ttctccgggc cgacggggaa gaggttccgg tagatgacgt
     1861 agccggcagc accgggcgtt gcgtcccaca tcagccgcac ggcctggttg gtggccggct
     1921 cggcgagtcg aagaccttcc gtgagcggca gcaccgatga gccgtcccag ggctggtagg
     1981 gctccaccac ggcggcacgc cggtccgtgc ccggtggctg gctgatctgg tccacgtcct
     2041 gggccgccgg gtccacggtc cacacgaacg ggccggcgat ggtgaaggcc agcagcccca
     2101 gcacgatgta gagcgagaac agcgcgcggc ggttggcctt caggcggatc caggcgtcct
     2161 gccagtagga cagggacggc cgggagatgg tctggatccg ccgctcggcg tgaacgggct
     2221 cgaagtctcg tgcggtgagg gcagtctcgg ccatgggcta ctcgagcctc acgcgcgggt
     2281 cgatgagccc gtagagcagg tccaccacga tgaccatgaa gaccaggaac gagccgtaga
     2341 ataccgtggt gcccatgatc accgtgtagt cgagctgctg aacggcctgc acgaagtagc
     2401 ggccgagacc gggaatggcg aagacgagct ccaccacgaa gccccccgtg gtgatggcgg
     2461 cgatggacgg gcccagcacg gtgatcacgg gcaggatcgc gttgcgcagc tggtgcctgg
     2521 tgaagatgcg cgtgggcggc agccccttgg acttggccgt gcggacgtag tcggcgttga
     2581 cgatttccag catggaggag cgcatgagcc gcgtcaggta agccatggtg ccgagcccga
     2641 ggaccatgga cggcaccagc atgtgccaca cgctgcccca gccggccacg ggcagtatcg
     2701 agacgcccag caggtcgttg aggttgacca ggctcagctg gctgatggcg gcgatgacga
     2761 agctcggcac ggaaatgccc aggatcacca ggaacatgat gatcacgtcc ggcagccgat
     2821 tgcggtacag ggccgtcagg gcgccccaca gcacgccgcc gaggccggcg aacaccaccg
     2881 cgaggatgcc cagggtggcg gagacgggga agtgctcacg gatgatgtcg ttgacctcgc
     2941 ggttctcctg agtgaaggag atgccgaaat ctccttttac gatgttgccc atgtagatca
     3001 ggtactggtc gaaaacaggc ttgtcgaggc cgtattttgc ttccaggttg gcgcggatct
     3061 ctgccgtgac ggctttctcg tcggccaagg ggtcgccggg cacgctgtgc atggcgatga
     3121 aggtggcggt agctatgaac cagacggtga ctatgccctg gagcagacgc ttgagcgtgt
     3181 agttgagcat gtcttaagcg tcagccttcc acgatgtagg cgttggtgaa gtccgggtcc
     3241 gggcccacgg agcggcgcac gagcccgccg aggtcggggt tcaccacgta ggtcgtgccg
     3301 ggctcgtagt tgatcaggat caccacgtcg tcgtgcagga tccgctgtat ctcgccgaac
     3361 gcgtccatcc tcgtcttggg atccagggac tcctcggcga tggctacctg gcgatccagc
     3421 tcggggttgc tgtagcggcc ccggttgttg aggttccagg aggcgaacag gtcgccgaag
     3481 gtgagcgggt cgtcgtagtc cgggccccag ccggctagca ccatgtcgaa atcgcccgaa
     3541 gtcatcttgg ccaggcgctg cttgaagatc tgcttgtcta tgcggatgtc gagaccgagg
     3601 gtctgcttga acacctgctg gaagtactcg gactgaatgt tggagacggg attgtcgccg
     3661 cttagcagca ccagcggggg gatctgctcc atccccagct cccgcttcgc cgcctggaga
     3721 tactctctgg ccttgaccac gtctttgatc gtcttcggcg ccgggtactc ctggcggaag
     3781 taaccgttca cgccgcgcag ccagacgggg aacagcgatt cgccggggat gtagccgggc
     3841 agcttggtca ccttgtagac cagctccgcc gggtccacgg cgtgctgcat ggccttgcgc
     3901 aggttcgtgt tcgcggtgac gcgcccgtcg cgaaagttga actcgatgaa gaagaccgag
     3961 ccgtccatga atcgcttgat gtgccagcgc tgctccatgg cgtcgttgag attctccgcc
     4021 agcagcccgg cgtaggcgac cttgttgtcc ttgaagagat tcagcgtcgc gttggcgtcg
     4081 gaagtgatgt acggaaagtc gagcacctcg atgcggatcc gttcctcatc ccagtacgtg
     4141 gggttcttct ccagcctcac gtgggcgccg tgcacccagc gcgtcatgcg gaacgggccg
     4201 ttgtagagca gcgtgtgcgc atccgcgccg tagcgaccct tggttgcctc gtaaaaatcc
     4261 ctgcggatcg ggtagtaagt cgggaaagcc acgagcttgt cgaagtaggc caccggccgc
     4321 tcgaacgtga cctcgagggt gcggtcgtcc acggcccgcg cgccgagctg cgaaacgggg
     4381 agcttggcct cggcgatggc ccgcccgttc ttcaccgggt agaggatgaa ggcgtactgg
     4441 gacgcgttgg cggggtccag ggccagctgc caggcgaaca cgaagtcgtg ggcggtgacg
     4501 ggcgcaccgt cgctccacac cgcgtcgctg cgcaggtgaa agatggcgcc gctttcgtcg
     4561 atctcccagc gctcggccat acccggcacc agccggttgt gctcgtcgta gcgcagcagg
     4621 ccttccatca cgtggcccag caccatgccg ctgaccatgt cggtggccag ggagctgttc
     4681 agctgggggg gttcctcgcg caggaagagc gtgaccgtgc gtgtctccgg atccacggca
     4741 acctcggagc cagcgccgga cccggtcagg ctggaagccc aggccagcgc gtacatcagc
     4801 agcccgatgc tgccgatgct gatggcggcg aacaggagaa gctgccgccc ggcggaccgg
     4861 ttgaaggtgt cctcgcttcc tgcgaccgtt gtcaccgcgc tccgcttcct actgaacgac
     4921 ccgcagcaga ttacccacaa tgcgctcgag atgcatgagg ctgttgcact cttctacctg
     4981 atgacagtac gccgcgtacc ggcgcatctc cgagtcccct acgccccagg cgttacgcgg
     5041 ctcgggattc agccagatca gccgcttgca gcgatcgtag agctccttta agagctccgt
     5101 gtgcggatcg ccgtaattgt tgcgggcatc gccgaggatg atgatggtcg tgcggttgtc
     5161 cacctcgtcc aggcagagct tcttgaagtc catgagcgac tggccgtagt ccgtcgagcc
     5221 gccactgtag tcgcggatgg tcttggcgat ggcgtcctcg atggcgttgc gctcgaagag
     5281 ctcggtcacc tcggccagat ccgacgagaa ggcgaaggag cgcaccttgg gcatgacctc
     5341 ctccaggctg tacaggaaca tcagcatgaa gcgcgcgtag ttggcgacag agccggagac
     5401 gtcgcagatc gcgaaaacct tgggccggtc gatcttcaca gatttccagc gcaggtcgaa
     5461 gatggctccg tcgtaggcca tgttgtggcg cagggtgcgc ggaatgttga tctgccccct
     5521 cttgaacact ttcttgcggc gcgaatgcag agcggccagc ttcttggcca tccggaagac
     5581 gatttcctgg atgaggcgga agctgcgctg ctccacgttg gacagcttta ccttgcgcag
     5641 cagctcttcg cgaagccgct tgcctgacgc gtcggcgtga aggagaaact gtttctccac
     5701 gtagtcgcgc acttgctcgc gcagccagtc ccggcgccgg gtgagctcct ggccgaggcg
     5761 gcgatcaggc acggccgggc gctggcgcag ctcggaaatc tcccggttca gatccgcaag
     5821 gcccatctcc tccatgatcc ggcgggtgta gagccctttc tgagtgaaca cctcgatgtc
     5881 gcggacgtcg acggcctctc cggccgccgc cgttgccacg gacagctcga ccttggaatt
     5941 ggccatgagc agctgcccga gggtcgactg cggcttgctc atcgcgccgg cgcccagatc
     6001 ctcgtcctcg tctgcctccg cgtcggcgct tgcgcccggc ttctgttttt tcttcgcgct
     6061 gccagcggca tcacccgggc gcgcttcgcc gggctgagcg ccgccgcctt caccgccttc
     6121 ctcgccgcct gcggtggtct cggcgtcgga ggcgcgctca gcctcgccgg ccgctccctg
     6181 gctgccttgg atgtcggtga aggagaaata cgcatcgaag cactgctcga acgtctcctt
     6241 ctcgtccgcc gtcttgggta gcaccagcgc aagcgagcgc ttgagcgcgg cccgatcccg
     6301 atagcccacg agctccacgg tgttgaaggc gtcgagcgtc tccgccgtgg agatgcgcac
     6361 ctcggaattg cgcagcgcgc ggatgaagtt ggtcagggtc cggtccatcc ggacttattt
     6421 gagcgccttg ctggtcagcg tggtcacttc cgcgtccacg ttgtcgatgt cctcctggaa
     6481 cttgaggatc acgttgaggg tgtcccggac cagcttgggg tccagcgact cggtgtgcag
     6541 gagcagcagc gtgcgcgccc agtcgatggt ctcgctgatg gccggcacct tcttcaggtc
     6601 cagatcgcgc agctcgtgaa tgaactgcac cagctggcgt ctcagctccg gcgggatctc
     6661 cgggacccgg gcgtggatga tgcgctgctc gagatcgacg tctggaaacg gaatgtacag
     6721 atgcaggcag cggcgcttga gcgcgtcgga gatctcccgg gtgttgttgc tggtgagaaa
     6781 caccatgggc ggctgccgca cggccttcac cgtgccgatc tcgggaatgg acacctggaa
     6841 atcggacagc atctccagca ggagcgattc gaactcgtga tcggacttgt ccacctcgtc
     6901 gatgagcagg atgcagccgg cttcctcctt cagggccttg agcagcgggc gcggctctaa
     6961 aaactcctcg gagaagaaga tgtcgccgaa ctcgtgcagc cgcgtcacgg attccgcaaa
     7021 gcccttcgct ccctgcagaa cctcgtcgag cgtctccttg agcacctggg tgtacaggag
     7081 ctgcttgccg tacttccact cgtagatggc cttggcctcg tcgagcccct cgtagcactg
     7141 cagacggatc agcggcagcg caaacatctc tgccgtcgtc ttggcgagct ccgtcttgcc
     7201 cacgccggga ggaccttcga tgaggatcgg tttgtgcagg tggtaggcga ggtagacggc
     7261 cgtggcgatc tggtcgctgc agatgtagcg atgtgactcg aggccgtccc tgaggctgcc
     7321 cacggactcg gtgagctctt cgatgttcac tgccgcttcg gccactgctt cccccagccc
     7381 ggctcttacc tgggcgtgcg tatttcgcgc tgcacaaaac ccgctattat cgctgctccc
     7441 cttcagcctt gtacaccgcg ctggcgagcg atccgttggc catcgccgat cagtcggtct
     7501 cgtgaagccg gatcaggcct tcctgggcgg ctgacgccac cagctctcct gcgcggttgt
     7561 agatcaggcc acggttgagg ccccgggcgc cgctggcgct ggggctgtcc tgaacgtaga
     7621 gcagccactc gtcggcacgg aaaggacggt gaaaccacat ggcgtggtcc aggctcgcca
     7681 tctgcatgtt gtcctgcatg aagctcaccc cgtgggggag ggtggcggtg tccagcaggg
     7741 accagtcgga aaggtaggcc agcacgcact ggtgcaggcc cgggtcatcg ggcaggcggt
     7801 cgcggcactt catccagcac atctgctgtg gcggccgctt ctccggcgcg aagacgtcca
     7861 gcgggtcgat gggacgcacc tcgatgggcc tttcccggtc gaaggccgcc tccagcggct
     7921 cggggagctt gtcggcccat gccttacgca gctcgctctc gtcgggcagc ccctctggat
     7981 ctggcacttc gggcatgggc atctgatgag acagcccggg ctcgtcgatc tgaaaggaga
     8041 tggccatgtt gaagatggcc ttaccgtgct gaatggctac cacacgacgg gtggtgaagc
     8101 tgcgcccgtc ccggatccgg tcgacggtgt acaggatggg caccgtggtg tcgccgccgc
     8161 gcaggaaata gccgtgcagg gagtgggcgt gacggtcctc gggtacggtg cgtgaggcgg
     8221 ccaccagggc ctgccccaga acctgccccc catagacccg ctgccaaccc tcgctggggc
     8281 tggtcgcgcg gtagtggttc ttctcgatct cctccagatc gaggagatcg atgagatcgg
     8341 tgacggtctg gtcctggctc attggcggct tctggcgggc ttggagggag cgctattgta
     8401 gtcagccgac ccgccgggga gaatggcgat cgccgcttta accgccgccc cgaggagttg
     8461 cccggatgcc gtcacgccgc gagctgatcg ccctgaccga gggagagatc agggactacc
     8521 tggccgagca gaagacgctg atcatcgtgt ccaacggcaa ggacggctac gcccaccctg
     8581 tgcccatgtg gttctggacc gacccggatg gctgcctcta ctgcaccacg ttcagcaagg
     8641 cccagaaggt gctcaacttc cggcgcgacc cgaaggcgtc cctgctcgtg gagagcgggg
     8701 acgagtatac ggagctcaag agcgtgctga tctatgccaa agcggaggtg atcgaggagt
     8761 tcgacgcggt ccgtgaagcg ctggtcaaca tcagcaccaa gggccgtggc gacgtgtccg
     8821 aggcggagcg cgcccagatt cgtgatgcgg tagggaagac ggcggagaaa cgcgttctgc
     8881 tgaagttcac gccggagcga tacgtcagct gggatcacgc caagctcggc gggcgttact
     8941 gaggcgcagg cttcaggccc cccgcggccg ctgggcgaag aggtaggcga ccgccgccaa
     9001 tcctgaagac ccgaaaatca tgcacatcac cacgatctgg tagcgcacgg caatcagcgg
     9061 atccacgccg gacaggatct ggcctgtcat catccccggt agagacacga gacccaccgc
     9121 cagcagggcg ttgacctggg ggatgagcgc ggcatccagg gccgtcgtgc gtgcctcggc
     9181 gtactcgact tcccgggcct gctcggactc gaaccgctcg gctgcgaggc tcaccgtgtt
     9241 catggcgttg gcgaaaatca tgccggccag cggcaccacg aagcgtggct cgaaccagcg
     9301 gggcaggtcg atgaccagct ggctcaccag ggccagcatg gccaggccgc agatggatat
     9361 ggcggtcagg gactgcagat acttgcgcgc gctgcggctc tgtaggggtc gcagcgcgat
     9421 ccaggcagcg acgcccatca tcatcacgag cacggcggcc acgatccacg ggttctcggt
     9481 ctcgaacacg aacacgagca cgtagccgat cgccaggagc tgcacgagca tgcggatgtt
     9541 ggcaatcagc gcctgcccgg cctggaggga ccaggcgcgc atcaccgcca cgagagccag
     9601 actcggcagg aaggcgacca gcagggcgcc gatggagacc tccgggaggc cctgcactgt
     9661 cgctatgttc ccgggcgcta cgggtcaggc gcccagaacg aagagccaga tcaggctgac
     9721 cagggagccc atgaacatca gggagcgcag ggtcgcccag ccgcctatgt aacagacccc
     9781 atgcagcacc cggaacacca cgaaggccac ggccagctgg gccgatctct ccgggtccgc
     9841 ccccaggacg tgggcagtga gcgccgcagc cgtgaacagg gccagggcct cccaggcatt
     9901 ctgctgcgcg gcgtaggccc ttgagccggc acccgtcaac gacttgatct gctctcgggg
     9961 gtcgtcgttg tccagcgtgc cgagctgctt gtttcggaag taaccgccga cgccggccag
    10021 aacgatgggc atgagcatca cgatcagcag gcaccaaagt ggggtggtca tgtctgtctc
    10081 ctagtacttg tacgattccg gtttgaatgg accgctttcg tccacaccga tgtattttgc
    10141 ctgctcggcc gtcagtctgg taaccacccc gccgaacccg gcgaccatga gctcggcgac
    10201 ctcctcgtcc agcttcttcg gcagcacctc cacggtgatg ggcgcgcgct ggttctccgg
    10261 ctggtcggcc cagcgctgct cgtagaggtg catctgagcc agcacctgat tggcgaagga
    10321 gccgtccatg acgcgcgacg ggtggcccat ggcgttgccc agatttacca ggcggccctc
    10381 ggacagcagg atcaggtagt cgtggcgatc gtcgcttcgg aaaacctgat gcacctggtc
    10441 cttgaccatc tcccagcgcc agtgctcacg catgaaggcg gtatcgatct cgttgtcgaa
    10501 gtgcccgatg ttgcagacca cggcgcctgc cttgacggtg ctgagcatgc tggcatcgca
    10561 gacgtcggcg ttgccggtgc aggtgacgat gagatcggtg tcgtgcagca ggcgggtatc
    10621 gatgttctcg atgcggccgg tgttctcgcc gcccaggtag ggagaaacca cctcgaaccc
    10681 gtccatgcaa gcctgcatgg cgcagatggg atcgatctcc acgacgcgca cgatcattcc
    10741 ctcctgacgg aggctctggg ccgagccctt gccgacgtca ccgtagccga tgaccagcgc
    10801 gcgcctgccg gccatcagca tgtcggtgcc gcgcttgatg gcgtcgttca ggctgtggcg
    10861 gcagccgtaa cggttgtcgt tcttggactt ggtgaccgaa tcgttgacgt tgatggccgg
    10921 gaccttcagc tcacccctat cgatcatctc gtagagccgg tgcacgccgg tggtggtctc
    10981 ctcgctgacg ccatggatgg agtcgagcat ctcggggtac ttctcgtgga tcacctcggt
    11041 aaggtcgccg ccatcgtcga gaagcaggtt ggcgtcccac ggcttgccgt ccttgagaat
    11101 cgtctgctcg atgcaccacc agtattcctc ctccgtctcg cccttccagg caaaaacggg
    11161 tatgcccgcc gccgccatgg ccgcggcggc atggtcctgg gtggagaaga tgttgcatga
    11221 cgaccaacgg acttctgcgc ccagggcgac cagggtctcg atgagcaccg cagtctgcac
    11281 cgtcatgtga atgcagccga tgaccttcgc gccggccagc ggttgctggt cggcgtagcg
    11341 gttgcgaagc gccatgagcg ccggcatctc ggcctcggcg aggttgatct cgcgcctgcc
    11401 gaaatcggcc aggctcatgt cggccacttt gtagtcagtc atctgctctt tccttttcga
    11461 tgtgttgtct gaaatggttc gatgggtttc cgtatcccgg ggctaggtgc cgttgcgggc
    11521 gtagccgtgg atctgtatct gaaagccgtt tttctgggcc aggtactggg actcgaccgg
    11581 cgccaggccc gcctgggccg cccactgctc cagctcgccg ggctcgaagc ccagccacag
    11641 gtcgccgcac gcgtcggtga cccattcctg ctcgtggcga tcgagctccg ccacgatgag
    11701 cagaccgccg tgccgcagga tccgcgaagc gtggcggaag aatctctggg gcgacgccag
    11761 gtgatgcacg accatggccg cgatcacggc gcggtatcgg cgaatcgccg gtagctcggt
    11821 gaaatccttc tggcgcagct tgacgttggg cagcccgtcc agacgctcgg ccgcctgggc
    11881 gagcatggtg gaggagctgt cgattccgat caggtcgtcg aagctccctg caagcgtcgc
    11941 aagcagctgc cccccaccgg ggccgacctc gatgatcggc ccggaatcga cccctgcatg
    12001 gtcgagcagc tcgacgatgg ctggaacgta tacctgcgct tcgcagatga gcgcctgctg
    12061 atcggcaaaa cggtgtgcgt tttcggcaaa gaagttgttg ctgcgctggc ttcgctcggc
    12121 gtgaatctcc gccgcccgct ctgccagggc ggcacccagg ggggtgccgt cgacggtttc
    12181 gaaaaggcgc tggcgcaatc cgtcgtcggg ttcggtgcac cgccggtagt agacgttgtt
    12241 gccctctcga cgtttcgcga ccaggtttgc cgtcagcagg actttcaggt gatggctgag
    12301 cgccggttgg ggaatctgaa agaggtggca cagctccgtg acgctgtagg atccgtcact
    12361 gagcacccgc aggatgctcg cccgcaggtg atcgcccacg gctttggcga cctcgacgac
    12421 ctcgtcgagg ttggcttgct ggcctgccgt ctggcccatg ggcggtggtg cctctacgca
    12481 acgagaggcg atcctatcag cgccttcgca caatatcaaa aaattttgat attgatggcg
    12541 gaaagatcgc cgtgtcagaa tgcggcgatg agttggagct cgccggatct cgtcagcaag
    12601 ctgccggacg tgcagaccac gatcttcacg gtcatgtccg cgctcgccag ggaggagggc
    12661 gcgctgaacc tttcccaggg tttccccgat ttcgatggcc cgcccgagct cctcgaacgg
    12721 gttcagtatt acctcaccca cggtcacaac cagtacgcgc cgatgacggg cgtggcgccc
    12781 ctgcgcgaag ccacggcggg caagatcgcg gatctttacg gctgccagct ggatccggag
    12841 caggaagtga ccgtcacctc cggcgccacc gaggcgctct tctgcgccat agcggcggtg
    12901 gtgaatgccg gtgacgaggt gatcgtcttc gatcccgcct acgacagcta cgcgcccgtg
    12961 gtgaagctcc agggcggcgt gacccgccac gttcccttgc atccgccgga ctatcgggtc
    13021 gactgggatc gggtggcgga caacttgagc gatcggacca ggctgatcat catcaacacg
    13081 cctcacaacc ccaccggaac ggtctggggc gaggacgaca tcgaggcgtt gcgcgccgtg
    13141 atcgacggcc gcgacctgtt tcttctcggc gacgaggtct acgagcacat cattttcgac
    13201 gggcgccagc acgcgagcct gtgccggtac ccggatcttt tcgcccgcag cttcgtggtc
    13261 tcgtcgttcg gcaagaccta tcacaccacg ggctggaaaa tcggctactg cgcggccccc
    13321 gcgccgcttt ccgccgagtt ccgccggatt catcagttcg tcaccttcac ctccatcacg
    13381 cccgttcagc tcggtctcgc cgatttcatg acccggcatc cggagcacca tctcgggctc
    13441 ggcgcgttct acgaggccaa gcgcgatttg ttctgcagcc tgctggaagg ctcccgtttc
    13501 ggcctgacga agagtgccgg cacctacttc cagctgctcg actacggcga ctactccaac
    13561 gaggccgata cggatctggc cgtgcgcctc acccgggagg cgaagatcgc atccattccg
    13621 ttgtcggtct tctatgctga cgatcccggc caccgcatgc tgcgtttctg ctttgccaag
    13681 gatgacgcta ccttgcgagc cggtgcggag atactcttag gcctgtgaag ccgacgccgc
    13741 cggatgagcg cgcgcgaacg ggatgaatcc gatgaccgac atgaggagaa aaacggggtg
    13801 aaacgtccac ttctggcatt gctggtctgc tgcgcatttg gctgctcgga atcatccaac
    13861 gagacgcccg cgtcgacggc ggccgcaggt gacgggccgg ccccagccgc acatccggga
    13921 gaagagacct acaaccgctt ctgcttttcc tgtcacgccg ccgggattgc cggagcgcct
    13981 cgaaccggcg acgcgcgtgc ctgggcttcg cgggtggcaa agggggagga ccagctgctc
    14041 aagtcgacca tcgagggcat agcgcccggg atgcccgcca tggggctgtg caggcaatgc
    14101 tccgaggacg acctgcgcgc cgccatcgac tacatgatcc cggatgctta gccgccctcg
    14161 ccagatgttg gcgcaaacgc cacccggcca cagcttgatc gacaaaaaag gcaattatat
    14221 caatgggttg aatagtggaa cggatgttgc taaacgaatg ccgagacgaa ttgtcgaatc
    14281 atgagaacga cccgggtggc aaggagattg ccccccgcca actgacagga ccttaatcac
    14341 atgacccgct atcttcctgg agacatcgtt gcccgccgca agggtctggt gatgcacaag
    14401 ggtgtggtga ccggggacgg ccggatcttc cacaacaccc cgttccgggg cgagcacgtc
    14461 tgcaccgagg cggaatttcg cgccggcaaa cggctgtacg tgcagcgcca ggagttcgag
    14521 gcgcgccagc gcgcggtcga gcacgccgag cgccatggcg gtagcggcta caacctgttc
    14581 accaacaact gcgagcacac ggtgtaccgg gccacttccg gcgtcggtca cagcccccag
    14641 ctgaagtcgt ggatcgccgg gctggggctt gcgaccgtgg cctttgcggt cacccgccac
    14701 cccggcgcag cggccgccgg ctatgcgctg ggccgccggc tggccggccg gtcggccgac
    14761 cggcgacgct gatctagtcc tcggggcgct cgtccgggcg tcccagccag ccctcccctg
    14821 gggcggcacg gtacgtgccg aaccccggaa ccaccagacg gtgacagtgc agctgcagcc
    14881 gatcggcgcg ctgcatggga tctgtcggcc gcccgtagag gtgatcgccg acgatggggt
    14941 agccgatcca ggacaggtgc acgcgcagct ggtgggagcg accggttcgg ggggcgagca
    15001 gcatgagaga tctgtcgtgg ccgttggcga gcgtgcggat ccgcgtgatg gcgggatgtc
    15061 ctgccgtatc cgttgcggga aggacccagc gcgtgccctg ctgccggatc tgctcccgcg
    15121 ggccggcgac ccgaaaacgg cttttccggc cgcgttgcaa gggcaggtcg atggtgtggg
    15181 tcgatgtcag cgccatggaa ccgaccaccc aggcaacata gaacttgcgc acggcccgct
    15241 gctgaaaggc gcgggtcagg tcacgctggg cgctccgggt cagggccacc agcagcaccc
    15301 cgctcgttcc tttgtccagg cgatggacca ggtagggcgt ccggtcgccg agcgcctgcc
    15361 tcagcgcgtc ccagtagcat ggttctccgc ttcggtcggc gagcagagac acgttggcag
    15421 gcttgttgag gactagcagg tcggcgctgc ggtgaagtac gtcgattgcc ggggtcattg
    15481 gctggcttcg ggctggctct gctgggtagt gggcggcctc cgcggcaaag taacatacgc
    15541 gccatgaatc tgagagacct caagtatctg gttgccgtgg ccgagcaacg gcatttcggc
    15601 aaggcggcag acgcctgcca cgtcagccag ccgactctgt ccacccagat caagaagctc
    15661 gaggaggaac tcggcgtcgt cctgctcgag cggaccaacc gccaggtcat gctcacgccg
    15721 gtgggagagc ggatcgtcgc ccaggcccag cgcgtgctgc gggaggtcga cgacctggtc
    15781 cacaccgcgg agcaatttcg ggatcccttc ggtggagagt tccggctggg cctcattcca
    15841 accgtggcgc cctacctgct gcccaggatc ctcggcccca tcaggaagtc cttccccaac
    15901 ctcaagatcc ggctgaccga agggcagacg gcggtggtcg gcgagcggct gcgtgccggt
    15961 gacctggacg ccgtgatact cgcgttgccc ctggaagagg agaacgtggt ggaagccgag
    16021 ctctacaggg agccgttcta ttttgccgct tcgaagaacc atccgaaggc gggcaagcgg
    16081 gaagtagcgc tcagcgatct ggatcaggag caggttctgc tgctcgagga cgggcactgc
    16141 ctgcgcgacc aggctctcga ggtctgcaat tctcagaacg ccgttgagaa cacgaacttc
    16201 cgcgcgacca gcatcgagac gctgcgccag atggtggcgg cggacgttgg cataacgctc
    16261 atgccggagc tggccatacc gcagaagcac ggcgccgtga gatacattcc gttcaaaggc
    16321 aacgggccgc accgccgaat cggcctctgc tggcggggca gctccagccg ggcggccctg
    16381 ctgcatgaca tggctggcgt tcttcgggaa gtcatggccg gcggctgacg gtgcccgcgc
    16441 ggcgggccgt ccggttcagt ccaggtcccg cctcaggctc tctgctctgg ccgggtccag
    16501 ggtcaccgct cctttgagcg acgcgtggtc gatgcctgcc aggatgggcg cgccttcctt
    16561 cgccgcctgc acggaagccc cgtcgagctc gaagcggaga aaatgcacgg ccgccgcctt
    16621 gcccgcttcc gaacgcggca tgtcctcgtt cgcgatcgcg tagatgcgcg gcccgtcgcc
    16681 gatctgcacc cagatggtgt cctcgatccc gcccatggtg gccagcgcac ggtgccgttc
    16741 ctccacgtcc gggtactcga tgagaaaagt cgccttccag tttgcgccgt cgggaatcag
    16801 cgggttgtag gttgtcagct cctcctcgat ctccgcggcc tcgaagatct tctccacgcg
    16861 aagcatctcc tggatctggt acttcatggt gagagcgtct tcgaagcaca gcgtggcatg
    16921 ggggccgatg gctacgcggc gggccttttt gtgagcgatc acctccgcgc ggaacgccgg
    16981 gcgctcctcg gaatactgct ccaggctcca cagatcggtg cggttcagtt tctgcatggt
    17041 tcaaactccg taggcgcgac ggaccatggt gattggatgc tcggcggccc catcatcctc
    17101 cattccgtgc gcgatcagcc gacccgccat ggggcagtca gagccgaaag tgtcggcttc
    17161 ggcctgcttg acccgattga ccacgggccg ggcgatcttc atggcctttt cgtaggtttc
    17221 acttttgacg gcgtaggtgc cgtcatgtcc ggagcagcgc tcgatggcgg tgacctccgt
    17281 gtcggggatg aggctcagga actcccgtgt cttcatgccg atgttctgca cccgctggtg
    17341 acaggccacg tggtaggcga ccttgcccag gggcttgccg aacccggtat cgatcagccc
    17401 ggccttgtgg cgcagcacga ggtactcgaa ggggtcgaag aaggcctttt tgacctgctc
    17461 gacatcggcg tcctcgggga acatgagcgg cagctcctgt ttgtacatca gcacgcacga
    17521 ggggatcggc gcgatgatgt cgtaacctgc ctcgatggcc tccaggaaca gcggcaggtt
    17581 cttatccttc aggctcgcga ccttgctcag gtcgccgagc tccagcttgg gcatgccgca
    17641 gcatttggcg tccttgagaa tctttacggg aatcccgttg tgattgagca ccgcgatgag
    17701 gtcttcgacc acctggggct cgttgtggtc gccgtagcag gtgatgtaga tggcgacctt
    17761 gcccgtggtt ctctcgcagg gtttcggagt cagcgtcgcg ccgatttctg tgtccagacg
    17821 cttgttcagc ggtcgcgacg agaagttcgg cagcggtgcg tccgggtgaa tgccgaccgc
    17881 cttgtcgccc agcttgcgca gcgcgctgtt gcccgcagcg gcgtttgcca tctgggccac
    17941 gcccggaatg ctgagcgtgt cgaagatggg gtcggtgctg gtaatcagcc tgtcgcgcca
    18001 cttcgtgtcc ttctcccgga acttctgcgc tttcgcgcgg agcatgagat gggggaagtc
    18061 caccgcccac tcgtgaggcg gcaggtaggg acacttggtc tcggcgcaca ggtcgcacag
    18121 aaagcactgg tcgaccacct tcatgtagtc ggccttgtcg acgccgtcca cctccagggt
    18181 ctcggactcg tccaccagat cgaacagggt ggggaacgag tcgcacaggc tcacgcagcg
    18241 tcggcagccg tggcagatgt cgtagacgcg ctcgagctcg gcgtaaaggg aggccttgtc
    18301 gtagaactgc gggttctgcc aatccagcgg ttgtcggtct ggtgcctcga ggctgccttc
    18361 cttcatagcc atcttcactg cctttgagtt gcggagactg ggtcggtgtg aataagtcgt
    18421 tgtgaataaa aaggggccct atcaggaccc cctccgtttg gttggcggtg aggtccgctc
    18481 agcccatcgc gtccagcgct ttctggaagc ggttggcgtg agagcgttca gccttggcca
    18541 gggtctcgaa ccagtccgcg atctcgtcga agccctcgtc ccgcgcggtc ttcgccatgc
    18601 cgggatacat gtcggtgtac tcgtgggtct cgccggcaat ggccgccttc aggttgttgc
    18661 cggtggagcc gatgggcagg cccgttgccg ggtcgccaac ggtctccatg tactcgagat
    18721 gtccgtgggc gtgaccggtt tcaccctctg ccgtagagcg gaaaacggct gccacgtcgt
    18781 tctcaccctc cacgtcggcc ttggccgcaa agtagaggta gcggcggtta gcctgggatt
    18841 cgccggcgaa ggcgtccttg agattctgca gagtgttgct gtccttcagg tccatttctt
    18901 cttgcctctt actcgtttgt ctttctggtc gaatgaaccc gatcgggttc gcgtacggtg
    18961 cagtatacga actggaatcc cgagatccat ttgaaagtta ctatcggcac aatagatagg
    19021 aactatcaaa ccgggcttat tgctagattc tgcgaatgca gatgagaagc gttctcaaaa
    19081 ccggtgggag gtcaggtggc caggcgccac gatcgggcga tgccgacgat gctggtgacc
    19141 tgggtgaaga gggcgacggc aagcaccgcg cacgcgcctg gaattcccag ccactcgaag
    19201 gcgatggcac cggcgccggc gatgctcagc aaggcgatgc tgatctgtat ggccagagcg
    19261 ctgacgggcc cgcttttgcc gcgtcggcgc gagttgcgcg tgtcgagaaa gagaatgaag
    19321 cccgccagaa gagacccgga gcctgacgcc agcaggaacc ctgccggcag cgcggggtgc
    19381 atggccccga tcgccaggtt catcgtgatc agcaggccga agggcgcaat gatgtcgagc
    19441 gtggtgggct gccggaggtg cagatagttg aagctcaggg cgaaaccgga ccacatcgac
    19501 agcgcggtgg ccagaaccag cccggcctgg agccagaaca gccactgcag ggcatcgctt
    19561 tcgaagggct gcgggcgttc ccagtaaacg ctcagcaggt tctccaggat cagggcgact
    19621 acgatggaca gcagggtgag ctgcacgctg gcgaagtgac gtcgcacgcg gtctcgactg
    19681 gattccgcca tttgcagagt ctccgggtgg ttgcaatgac tacgctacct taagaaggta
    19741 gcggtcgtga acatcgaact ctggatcatc atcacggtcg cggccgcgtt tctgcagaac
    19801 gttcggtcca tgctccagaa gcgtctgacc caacgccttt cggtgaacgg cgcgacctat
    19861 gtccgcttcc tgtacgcgct gcccatcgcc tggatatacc tggccgccct ggcgtcacgc
    19921 caggacctgc cgctccccaa ctcggaattc ctgctttact gccttggcgg cggcgtgggc
    19981 cagattctgg ccaccgcctg tctcgtcggc tctttcgcgc agcgcaactt tgccgtcggc
    20041 accgcgctct ccaagaccga ggtggtgcag gccgccatct tcgggctgct cgtgctgggc
    20101 gatcccgtcg gctggcgggt gatgatgggc attgccgtga gcctggttgg tgtgctgctt
    20161 ctttccaacc gcgtgcgcct ccaggacatt tttcaggctg atcgctccgt gctcctgggg
    20221 ctcgccagcg gggccgcgtt tgcggttgcc atcgtcagct ttcgcggagc gtcgctcgcg
    20281 ctgccctccg gagactacct gatcagggcg gcccttaccc tggcggtggc cgtctctctg
    20341 cagtcgctca tcatgggcgc gtacctcggc cggttcgaga cgggagagct gcgacgcgtc
    20401 gtccgggcct ggcgccctgc gtctctcgtg gggctcacgg ggatgctcgc gtcggccgga
    20461 tggctcacgg cggtgacttt agagagcgcg gcgttggtgc gggccgtggg gcaggtagag
    20521 ctgcttttca cgttcgcggc ctccatctgg ttcttcggcg aacgggtcgg ctggcgcgaa
    20581 gcggtgggcg tcgccctggt cgtgatcggt atctgggtgt tgctctaggc gtgcagggcc
    20641 gcagggtggt cgcgcaggat tcgttcccac tccatgagga accagggcgt gaactgctcc
    20701 ggccgcgtgt cgagctcccg tttcaggtcg tcccgggcga tccagcgcca gtcgccgatc
    20761 tcggtggtgt tgatgacggg tgttgcgtcg ctgtggccga cgtagacgga gcagagctcg
    20821 tgctcggcgc ccagcccgcg gtagtccgcc tggtattcga acttgtagag gaactcgagc
    20881 ggcgcggtca tgccgagctc ctgatgcagc ctgcggcgcg cggcctcgtg catggtctct
    20941 ccgcgccgtg gatggctgca gcagctgttg gaccagtagt cgggccacag ccgcttgccg
    21001 gaggcccgct gctggatgag cacctctcca gcggagttga gcacgaacag ggagaacgcc
    21061 ctgtgcagca cgccggcccc gtcgtggcag gcggactttt ccagaaaacc gacctcacga
    21121 tcatcgccgt ccacgaggat cagcagctcc tcctctgagg agacgatctc ggattttgtt
    21181 gctgggtgag acatgaagac cggctcggcg tgagaaagat cagtgtagcg gaattggcga
    21241 cggcggtctg cgtgtcttgt ctggcctgcc cgctctccgt agcatgatca cgttgcggcc
    21301 atcggccgcg cgatcagttt ttcatgagcg catacggaca tcccagggag caccgttttc
    21361 agggctctca ggtagagctt gcctggtacg aatggggcga ggcgccgcat cccaaggtgc
    21421 tgctcgtgca tgccaccggc ttccacgccc gctgctggga tcaggtggtc gctcatctcc
    21481 ccccgggttt tcacatcctc gccgtggacc tgcgcggcca tggccgcagc accaaacggg
    21541 gtccttacgt gtggagcagt ttcctggcag acctggaaga gtttgccgcc gcgctgagcc
    21601 tggcgggtgc cgtcggcgtc gggcactctc tgggcggtca ctgcatcacg cagctggcgg
    21661 cgcggcgtcc gggcgcattc gaccggctgg ttctggtcga ccccgtcatc ctggatccgg
    21721 aagcttacgc gcgcccgcga tatgccgacc tgcagtcggc ggaggatcac cccgttgccc
    21781 ggcgtcggga tcactgggcc ggctgggaag agatgttcga aaggttctct tcgcgcaagc
    21841 cgttctcgcg atggaggccg caggtgctgg aggactactg ccgccacggc ctcgagcccc
    21901 gtgcggaagg cggctggcgt ctggcctgcc cgcccgtggt ggaagcttcg atttacctcg
    21961 gaaacaaggg cagcgacgtc tacggcatgc tgcacgatgt cgacgtgccg gtagccgtgc
    22021 tgcgagcgcc cgggcgcgac cccgatgacc acgacatcat ggatttctcc aagtcgccga
    22081 cctggcccga gctggcggac aggtttccgc gggggcggga cgtctttctg ccgcggctcg
    22141 atcacttcat tcccatgcag gcgccggaac tggtggcccg cttcatcacc gacggagatg
    22201 cagacccgtg aacttcctat cctggctcct tctcgggctc gtcgttggca ccctcgccaa
    22261 gctgatcatg cctggccccg atccgggcgg ctttttcgtc accatcggca tcgggatggc
    22321 gggcgcgttc atcgggggtt acggcgccca cctgctgggg atcggcgacg tggccggatt
    22381 cgatctggct agcctcggcc ttgccaccgc cggcgcggtg ctgctcctgg ggacctaccg
    22441 ggtcgttcgc ggcgggttct gaggccctgc ggctcgagtc ttggtcgcgg gggaaacgta
    22501 gccccacagc ccgctgcgcg tggtcccgat caggaagatg agcagagaga tggcgatgcc
    22561 gggaagcatc gcggtcagcg gctcggtctc ggcgatcaac acgcccagca ggatcgagct
    22621 gatgggcgag gacaccacga agctcaagag cagcacggag aggatctgcg ctctgtgatg
    22681 ttccggcgcc agctcctgca ccgtggttct gaccagggtg gtggtgacgc ccatgttcag
    22741 gccccaggcc acgatcagca cgtagaacag ccacaggctc ggttgcagcc ataaaaggcc
    22801 caggatgaac atccgggtta gctgcagtag caggtagagc cttcccggat gcttcagcgg
    22861 catgaaggcc agcaggagca cgttggagcc gatgctgccg atggtgaaca cgatcatcac
    22921 gttcgccagg aacgcggcgt cccccgcata gacctccttg acgatgtagg ggataccgat
    22981 gatgtacgcg ccggcattga acaggctgga gatgaagttc agaccgatga tgttgcggat
    23041 cagcgcggtc tcccacacgg cccgcaggcc ttccgcgaag ctcggccggc gcaccccgcg
    23101 cgcgctgatg ctgggcaggc gcggcaggcg tgccaccgcg aggagaccga ccgcgaacag
    23161 cgccgcctgg gcggccagga cggtgggcag accgagcgtg tcgagctggc cgccgagata
    23221 gacgccgctc aacccgacca gtgaggtgac caccatcatc agcgtcaccg cccgctgcag
    23281 gtccatgcgg gacacgcggc tgagcacggc ctgcctggcc ggatcgctca gcgcctggat
    23341 gctggccatg agcacgccga acagaaccac ccaccaatag ctgagcgcgc cgttgaggta
    23401 aacggcggcg atcagcagcg ggggaacggc catgagcagg tgcatgactc ccagataggc
    23461 ccgattgtcg aaccggtcgc ccagcaggcc gccgagcagc aggacggcca gcggtgggaa
    23521 ctcggccagc gatcgggcga ggcccgcctc atccgccggc cgctccagga tgcccactaa
    23581 aagccaggtg aaaaggaagc cctgcaggct catgcccgcc atccacaggc tggagctggt
    23641 caggtaccag gggtaagcgg tgcgtggggg cagcgcgatc tcggacaagc tgggtgcctt
    23701 ggtggtgtcc tttgccgggc gggcatcata accttagcgg tttccggcgg cttcagccgg
    23761 gttgaggcgg gccgtttctg cactgactgg gaggtggacg agatgaatat cggggcggtg
    23821 atggcgttca accacgtgat gagtccgcaa ttcatggcgg acgcggcgca gatggtggag
    23881 aacgccggtt tccactcggt ctggctgccg gagcacgtgc tgttctttcc ggactacgaa
    23941 tcgcgttatc cctacaccga agacggccgt atcccgggtg atccggaagg cgtcctcgat
    24001 cctttcacgg ccctgacgtg gatcgccgcg gcgaccaagc gcatacgtct gggcaccggg
    24061 atctgcctgg tgccccagcg tcagcccgtc tacaccgcca agatggtggc ggatctcgac
    24121 tacctctccg gcgggcgggt ggacttcggc atcggtatcg ggtggctcaa ggaggagttc
    24181 gacaacctgc agatggactt cagggcccgg gctcggcgct gcctcgaata cgtggaagtc
    24241 atgcaggctc tctggagccc gggtctgtcc agctacgaag gcgagacggt gactctcaag
    24301 ccgtgtcact tcaaccccaa gccggtccag cagcctcatc cgccgatctt tttcggcggc
    24361 gagagcgatc cggcgctggc gcgcgtggtc tcgcatgggc aggggtggta cggattcaat
    24421 ctgagtccag aggggctggc cgggcgcaaa gcgcggctgg cggagattgc cgccgaggca
    24481 ggtcgggata tgaatgacgt ccgcatattc atcggcccca gcgggaagcc cgttacgccc
    24541 gagaccgtat cggagtacga ggacctgggc gtcgagcagc tcatcgtgcc ggtgatagcc
    24601 ggcaacctgg agcgcctggc gcagcgtatc gacaaggccc gcgccatgac ggggctctga
    24661 gccgccggca aacgcaggca tgacacgatc atgcggaata tggcgggtcc gggaactatc
    24721 cgtaacaact ttcctacaga ctggcagggg gtcacgccgc aaactggttc gacctgatta
    24781 gccgtctttt tgccgcgatg aatgtagggc gacgaaacga aaagcccaaa acctggttcc
    24841 gttccgagcg cgtcttcatg aacgacggca gctggttctt ccgcacccgc gaaggcgtgg
    24901 agatcggccc ctacgagacc cagtccgaag ccaccatgga agccggcatg ctccgagagc
    24961 tgctccgcga ccggggacac gacggcaagg gcatcgccgt gatccgcgaa ttcgtccgcg
    25021 actcctacgc catgggtcgc cccctcacgc ccaagttcga ctaaccccct gacccgccgc
    25081 ccacaaatcg gcgcataatg tccccccgac ccctgggagg agttagacat gccgcgtctc
    25141 aagcagatca gccgagccga tgcgccggaa cacatacagc ggatgtacgg ttttcttttt
    25201 ggcgaccgcg atccggtggc cgagccgggc actgatacgg gcactccggg aaactggtgg
    25261 acggtgtttg ccctggtgcc cgattgcttc gaccacgccg tcgccggctt tcagctttac
    25321 cgcagcccca agcgggagct ggagcctacc cttcgcgagc tcggccagac ccgggccggt
    25381 tatgcccggg gaagccgctt cgtgttctcg cagcactgca aatctctgcg ttatctgggc
    25441 ttcagcgaag agcagatcga ggccattccc cactggcagg cggccgactg ctttgatgag
    25501 gtcgagcgtc tggtgctggc tttcaccgac tgcctggtgc tggacggcgg tcgggtgccg
    25561 gagggcctct tcgccgcgct caaaactcac ctttcggacg aggagatctt ggagctcacc
    25621 tacatcacct gcatgtacga catgcacgcc acgatgtgcc gcgcgcttcg gctcgagtac
    25681 gacgacgtgg aagagcgggt gtccgagata gccgcaccgg aagatggcgg cgacatcctg
    25741 acggccgtgg atgaaagcgc cacgggcggt tcggcggagg attgagcgca tgaccatcga
    25801 ccacgtcgtt cgcggttcgg gacctgagcc cgtgctggtc atgcacgact ggaacgggga
    25861 tcgccgaaac tacgatccgg cgctgcccta cctcgacggg acgacgttca cctacgcttt
    25921 cgtcgacctt cgcggttatg gcggctcgaa acacatcgcc ggagagcact cggtggcgga
    25981 gatcgccgcc gattgcctgg gtgtggcgga cgccctgggc tggggtgagt ttcacgtggt
    26041 cggtcactcc atgacgggca tggcgaccca gcggcttgcc ctggaggccc cggagcgcgt
    26101 caggagcgcg gtggccgtct gccctgtgtc cgcagccggc atggccatgg atgatcagac
    26161 gcgggcgttc ttcgagagca ccaccaccga cgatgaagcc tttcgcgggc tgatcggcat
    26221 gatcagcggc tcgctttcgg gcggttgggt ggacgcgaag ctgcgctgga accgcgaggc
    26281 ggcggccccg gacgtgcggc tcgactatct cgccatgttt gccgataccg actttgcgga
    26341 gtcggtggag gggctcgaaa cgccgtttct ggtgatggtc ggagagcacg acgcggaagg
    26401 tctcgacgca gccgccatgc agaaaaccta ccttgcctgg caccccaatg cggagctggt
    26461 ggtgattccg aactgcggtc actaccccat gcaggagtgt ccgccgttct ttgtcagcac
    26521 tctggagtct ttcctgcgca gacgggcggg ctgatcgtcc tgccagctcc cggcggcgtc
    26581 aggctgccac ggcctgaagg tattcctcga tgcggcgcag atgaccgtcc gcgttcgtcg
    26641 gccccgcgtt gtgggtggcg atggccaggt gggtgcagcc catgtcctgc cacttgcctg
    26701 catgggatgc ccatcgatcg gggttgccgc ccgcatactg ggcctgggcc tgaataccga
    26761 atccctccca gggcagcccg gcggcttcgc ggtgagcacg gatcgtgtcg atgcattccc
    26821 tggccttcgg attcgcgccc atgaggggta tccagccgtc gccgagctcg gcgcagcgct
    26881 tcaacagcgg cgcggcgctg ccgccgaacc acaccgggac gctggcgccg gggcgcggat
    26941 tgatgctggc ggcgtcgatg gtgtgaaagg cgccgtcgaa ggtcaggctg tcctcgctcc
    27001 agagcttgcg catgagctcg acctgctccg cctggcgcct gccccgggta tggaagtctt
    27061 cgttaagccc cacgtactcc acctgattcc agccgctacc cacgcccagg cggaaccggt
    27121 tgttggagag aatggccacc tcggccgcct gctttgcgac cagcaccgtc tgccgctggg
    27181 gcaagatcag caccgtagtg atcatctcga gcttgtcggt taccgcggcg atgaaggcga
    27241 acgtggtgaa gggttcgtgg aaagccacgt ccttgtcgta cgcccccttc cagccgcctg
    27301 gacggtttgg atccgcgccc agcacgtggt cgtagatcag cagatgggtc gcgcccatgg
    27361 cttccactcc ctgggcgtag gccttgatgg cgccggggtc cgttccgatc tcgttgtgcg
    27421 gaaatacggc tccgaattcc attgacatcc cctcgagtcc tgcggcgaac gggcgagctt
    27481 agtgccctac ggaatggcgc gcaatggcgt tacaatgcgc ggccgttttc gaccgcatga
    27541 ccccaatggc cgacccagca caatcgagct catcgttctc gttcgtggac atggagcacc
    27601 agattctgga tttctgggag cagggccgtg tgttccagcg cagcctggag aacacgcggg
    27661 agaagaaacc ctacatcttc tacgacggtc cgccctttgc gaccggtctt cctcaccacg
    27721 gtcacatcgt ggccagcacc atcaaggaca tcgttccgcg gtactggacg atgaaaggcc
    27781 gctacgtgct gcgccgcttc ggatgggact gccacggcct gcccatcgag cacgagatcg
    27841 acaagcagct caacatgtct gcccagcaag cggtggcgga gatgggggtc gccggctaca
    27901 accagcagtg ccgcgccatc gtgcagcgct acgttgacga gtggcgccac acgatcacgc
    27961 gcctcggccg ctgggtcgat ttcgacaacg actacaagac catggacgcc tggtacatgg
    28021 agtcggtctg gtgggtcgta aagcagctct gggagcaggg gctcgtctac cagggcgtca
    28081 aggtgatgcc ggtttcaacg gcccttggga caccgctgtc caacttcgag gccaaccaga
    28141 actacatgga cgtgcaggat ccggccatca cggtgctgtt caagctcgta gacgaggacg
    28201 cctacctgtc ggcgtggacg accacgccgt ggacgctgcc gtccaacctg gccatctgcg
    28261 tgggtcccga tatcgactac gtccgggttc gggacccgca gctgggcgcc gacgtctaca
    28321 tggcggcgca gcgcgtagcc gactacggca gcttcgagga ggtggggcgc gtcaaagggc
    28381 gcgagctcgt gggtcgccgc tacgagccgc tcttccccta tttccgcgag gcgggcgagg
    28441 agggcgcgtt cgtggtggtg atggacgact acgtcaccac cgaaagcggc accggcctgg
    28501 tgcaccaggc gccggccttc ggcgaggacg actaccgtat cgcgcgggcc gccggtatcg
    28561 at
//
LOCUS       DBNHLM010000131        12923 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_856762, whole genome shotgun sequence.
ACCESSION   DBNHLM010000131 DBNHLM010000000
VERSION     DBNHLM010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12923)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12923)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12923
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_856762"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="AC16QF_06115"
     CDS             <1..260
                     /locus_tag="AC16QF_06115"
                     /inference="COORDINATES: protein motif:HMM:NF018172.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - aliphatic amine dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amine dehydrogenase large subunit"
                     /protein_id="HIR6448164.1"
                     /translation="GYDGSHKDPAPEIWLFDTGERERLARIELRAPAISISVTQHEKP
                     YLLVASTEATLDVYDAMSGEYLRSIYDLGFTPYMVHPVSYE"
     gene            272..799
                     /locus_tag="AC16QF_06120"
     CDS             272..799
                     /locus_tag="AC16QF_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine dehydrogenase light chain"
                     /protein_id="HIR6448165.1"
                     /translation="MNWLDRITQKTTRKVARATSRRGFLAGASAVLLGASATPLLPVA
                     RASESRVPPQSTGNPEDPGDPTQCDYWRYCAIDGFLCSCCGGTQNACPPGTEMSPLTW
                     IGTCRNPVDGKNYVISYNDCCGKSSCGRCLCNRNEDDRPVYRPSANNDINWCMGTTSS
                     VYNCSTAVVLGLATE"
     gene            803..1441
                     /locus_tag="AC16QF_06125"
     CDS             803..1441
                     /locus_tag="AC16QF_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HIR6448166.1"
                     /translation="MLKLYALFGDDGAESPFQLNQAQALEVLQERFPELRRYRQFLRT
                     GEQLGNLVLAPFRASAEFWLAEHDDPTPLMAALETAAEDVHEVFAVNPRVLIRSEEHN
                     ISGEPFHDSGFKATFLFDRKPSLSLEEFHDYWLTHHGPIAAQTQDAERYVQSHIRTAQ
                     RAYDGVTELFWPDYPTALASMASEQMTTDQANDAPNFVDGESLVVFLAREAN"
     gene            complement(1502..1930)
                     /locus_tag="AC16QF_06130"
     CDS             complement(1502..1930)
                     /locus_tag="AC16QF_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6448167.1"
                     /translation="MKGRTVCFAFLIAAAPHAIGDGPVDAERARLNYMLNCQGCHLPD
                     GSGVEDAVPRMNGFVGNFLHVPGGREFLVRVPGSANAALDDASLAELLNWILVTMSPG
                     ELPPSWVPYSAGEVAELRSAPLREVEKVRAGLVRSIQQHL"
     gene            complement(1927..2448)
                     /locus_tag="AC16QF_06135"
     CDS             complement(1927..2448)
                     /locus_tag="AC16QF_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine dehydrogenase light chain"
                     /protein_id="HIR6448168.1"
                     /translation="MNWLDKLSEKSTRGLAQQTSRRGWLAGIGTVLAGAASVPLLPMA
                     RASSGGQPKLEDVADTGDPLSCDYWRHCSIDGFLCGCCGGSHNTCPPGTEMSPITWVG
                     TCRNPVDGKNYVISYNDCCGKTPCGQCFCNTNQGDRPMYRPDKNNDINWCLGTESTVY
                     HCSTSVLIGLAVE"
     gene            complement(2468..3619)
                     /locus_tag="AC16QF_06140"
     CDS             complement(2468..3619)
                     /locus_tag="AC16QF_06140"
                     /inference="COORDINATES: protein motif:HMM:NF018172.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - aliphatic amine dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amine dehydrogenase large subunit"
                     /protein_id="HIR6448169.1"
                     /translation="MRVFVVFSVLLAASGMSLAADQFEAEPLGEVAQLPSPNPDHWVV
                     IHDLSFNHMREGKLFLVDPHADSVSGQLRGMMSADFVASFLVSARRNEYYVIESFHAR
                     GGRGGERTDVVTIYDPATLSVLEEIIIPPKRLSGMPNRYQVALLDDDRYMVVFNFTPS
                     QSATVVDLERRQVLGEVAVPGCMFAYPTGKRGFSSLCSDGGMLTTRLGEDGSVAGTDR
                     LAPFNDVIDDAMFARPAEIDGVFYFPTFTGNVAPIDMRGEAAALGERWSLTTASERAQ
                     GWRPGGLWPAVGDGNGRLYMLMHGHGAEGTHKDGGDQVWIFDAANGKRIGKIELARWG
                     IALSVSTGERPQLMVSGAEPVIDVYEADGTHVRTLGVDMSTPLLVHGVR"
     gene            complement(3815..4384)
                     /locus_tag="AC16QF_06145"
     CDS             complement(3815..4384)
                     /locus_tag="AC16QF_06145"
                     /inference="COORDINATES: protein motif:HMM:NF014534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HIR6448170.1"
                     /translation="MQIPLQITWRNIPKSEALEADIVAKAEKLEEFFDHILSCRVVVE
                     KSHGRHRKGNLYRLRLDIHVPDKEIVVTRDPGDDHAHEDMYVSIRDAFDAARRQLQDY
                     VRVRRGHVKPHDQHHVGRVLRRIAAEDYGFLGTADGREIYFHRNSVVNADFDKLEPGT
                     EVMFVEETMNEGVQAKMVSVGKHRFPEGS"
     gene            complement(4530..4928)
                     /locus_tag="AC16QF_06150"
     CDS             complement(4530..4928)
                     /locus_tag="AC16QF_06150"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6448171.1"
                     /translation="MDIQDRIDRDGGVLERLNISVDLSANAGVRLEMKVAEDMVNAGG
                     YCHGGLLFALADTACAYALAEAGLAPVTLDAGITYLRPATAGERIAAETEVVRAGRRI
                     GHCEVRLTREDGTLLAIYRGTCSNASSDSR"
     gene            5056..5994
                     /gene="trxB"
                     /locus_tag="AC16QF_06155"
     CDS             5056..5994
                     /gene="trxB"
                     /locus_tag="AC16QF_06155"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR6448172.1"
                     /translation="MTATHHRLIVLGSGPAGYTATLYAARANLRPALITGVEVGGQLT
                     TTTEVENWPGGHAELQGPELMAQMAEHVERFEARVINDYITTADLEAKPIRLQGDSQY
                     TCDALIIATGASAMYLGLPSEEAFLGRGVSACATCDGFFYRGQAVAVVGGGNTAVEEA
                     LYLSNICSKVYLVHRRDELRAEKILQDRLFARENIEPVWNHTLAEVVGDDSGVTGVRL
                     DGTHGAEPMDLDVTGVFIAIGHRPNTEIFAGQLEMEGGYITTRSGLDGNATATSTPGV
                     FAAGDVADHVYRQAITSAGFGCMAALDAEKYLDESV"
     gene            complement(6010..6876)
                     /locus_tag="AC16QF_06160"
     CDS             complement(6010..6876)
                     /locus_tag="AC16QF_06160"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF016032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HIR6448173.1"
                     /translation="MPSTCTARACGELHNASLDVSIDTGYPPVINSRREAELAFRAAR
                     DVVGDAGLMQLDHPSMGAEDFSYYLERIPGCVLLMQVASSDLGKDLGQSVARPAESCR
                     TLGMRLVGAGTHPFSTRLAAITPGPRYESMERTSGLLGHRQITFSTHVHLSVIDGDEA
                     LWLARQLAPYLPLMLALSASSPFWQGHDTRFAAFRPRILASRFGLDARVAVNENGDVM
                     TLREVARQTLDLVADTAARFGDTDHLSRLARAISAGLPSERQRRRFAATGSLRAVVDA
                     VIDELLRDPVVG"
     gene            complement(6934..9723)
                     /locus_tag="AC16QF_06165"
     CDS             complement(6934..9723)
                     /locus_tag="AC16QF_06165"
                     /inference="COORDINATES: protein motif:HMM:NF015644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HIR6448174.1"
                     /translation="MYVLLFVALLGCGAWLAHSGSRHHRKVQVWGGAAIVGSAFGLML
                     LMAFWAEMLWFDSLGYQDRFWTFVWVRLLVPLLGGAAAGLTGLVLTRGAGIYLRRGVA
                     FACAAAGVIWGLASWQPVLLFLNRMETGIVDPLLGLDTGFYLFVLPLLDGVFWLGLFV
                     ALISLITVLALREQDGVVMPGPAFAVPKGRLLIPGASLTLTVVLAFGQLLSVLHLLYS
                     KLGVVMGPGWTDVHVRLPALILMGAIFLVAGMLPLFEGFRGFMARLVARWEAIAPVNV
                     VAVVGAWAVIGGAWLLLVVLLPALTQWLVVKPNEITFEKPYIENNIEFTRRGFKLDRV
                     EERQFSADAALGPESLADNRHLISEVRLWDWRALDAVYKQFQEIRLYYEFVDVDIDRY
                     RLGDRYRQVMVAARELAQRNLPEQSRTFVNQRFKYTHGYGYTLAPVSDFTPEGLPNLL
                     VKDIPPVTDQPELEVTRPEIYYGELTIEPVVTNTLEPEFDYPSGDANIYTRYSGTGGV
                     VLSSFWRKFLFGWKFDGTVFLLSSYPTPESRVLFYRQVPERVQRLAPFLVFDEDPYIV
                     AVDGRLKWIVDAYTSSSYYPYSQAFDSEEFFGLSRDEGQARVRTVQRLDGANYVRNSV
                     KAVIDAYDGSVDLSVFDEEDLIIQVWQRALPGLFKPRSEMPASLVAHVRYPQDFLLAQ
                     GLIFTRYHMSDPDVFYNQEDVWVRATEKHYHDITPVEPYYVMWELPGSDEAEFVLILP
                     FTPKNRQVLIGWVAALSDGENYGRFIAYKFPKERRVLGPQQVETKIDQDSFLSGQLTL
                     WDQQGSEVIRGNVLAIPIDDSLLYVEPIYLRAETAAYPELRLVAVMQGDNLSYAETLD
                     EALLGLFTGRQLVGETPSRSVKGLAIEARMAFESYLEALGDKRFEAAGEALRRLSQLL
                     ERIDAAASVGEGNGP"
     gene            complement(9829..10251)
                     /locus_tag="AC16QF_06170"
     CDS             complement(9829..10251)
                     /locus_tag="AC16QF_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013319.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR6448175.1"
                     /translation="MSDDRAIASWSHFDHAADMGVRGVFARCFPAARLEMLFDVSHNT
                     CKVETHRVGDETRELFVHRKGVTRAFGPGHPSLPDPLRSVRQPVLIGGSMATESYVLR
                     GTKESERRAYSSACHGAGRAMSRRAATRRWRGWFPCSA"
     gene            complement(10378..11247)
                     /locus_tag="AC16QF_06175"
     CDS             complement(10378..11247)
                     /locus_tag="AC16QF_06175"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448176.1"
                     /translation="MGNVGFVGLGVMGYPMAGHLARAGYNVRVYNRTGAKSERWVTEH
                     GGSRVETPREAASGADVVCLCVGNDDDVRAVVYGPEGVLDGLADGSVLIDHTTVSAEL
                     AEELASAAGERGCGFLDAPVSGGQAGAENGQLTVMVGGEQTIFDSALPVMEAYGKSVR
                     RMGPAGTGQLTKMVNQICIAGVLQGLAEGLHFADQAGLDIESVVDVISKGAAQSWQME
                     NRHATMARGEFDFGFAVDWMRKDLAIALQAGRRLQARLPLTALVDQFYADVQSYGGGR
                     WDTSSLIQRLRRT"
     gene            complement(11293..12363)
                     /locus_tag="AC16QF_06180"
     CDS             complement(11293..12363)
                     /locus_tag="AC16QF_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR6448177.1"
                     /translation="MLKFHDLEVAGCTSAGEEALCLQIVVPPELREAFSGAAGQHVVL
                     KADIDGEEVRRSYSLVSSPGEFPLRIGVRLHADGRMSSYLSRLTPGARLSVMPPLGSF
                     ASQCQGPAKRRYAAFAAGCGITPVLALVRAKLAEEPGCRWTVFYGNRSAGRAMFLDEL
                     LGLKDRYLGRLALHFVMSREPSEAEMFNGRIDAAKVQASAGKLFDPAATDEFFLCGPG
                     DMIAAVGAALGKLGVAPDRIHSEHFTVESVAAADRDGEPPPRTGGAAEIIVIMDGRRR
                     SFTMSPDDESILDAALDSGIDLPYSCCAGVCSTCRTRVVEGAAEMAENFSLEDWEIEN
                     GYVLACQSRPTSSELVLDYDDV"
     gene            complement(12382..12873)
                     /gene="paaD"
                     /locus_tag="AC16QF_06185"
     CDS             complement(12382..12873)
                     /gene="paaD"
                     /locus_tag="AC16QF_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HIR6448178.1"
                     /translation="MVDEQPVRIIAAEAGEDSVWAVLRGVPDPEIPVLSVVDLGIVRY
                     VRMPGPQVGITPTYSGCPATEVIRASIRSALAQSGYPNARIEEVLSPPWSAAWISAEG
                     RRKLLEYGIAPPNPSSPDEPESCPHCGGRKIEMISQFGSTPCKALFRCLSCREPFDYF
                     KCH"
BASE COUNT         2446 a         4368 c         4008 g         2101 t
ORIGIN      
        1 acggctacga cggcagccac aaggacccgg cccccgaaat ctggctcttc gacaccggcg
       61 agcgcgagcg cctggcccgc atcgagcttc gcgcgcccgc catttccatc agcgtcaccc
      121 agcacgagaa accctatctt ctggtggcaa gcaccgaggc caccctggac gtttacgacg
      181 ccatgagcgg cgaatacctg cgatcaatct acgatctcgg tttcacgcct tacatggtcc
      241 accctgtgag ctacgagtaa ggagccgcgc gatgaactgg ctggaccgga tcacccagaa
      301 aaccacgcgc aaggtcgcac gagccacttc gcggcggggc tttctcgccg gcgcctcagc
      361 ggtcctgctg ggcgcatcgg caaccccgtt gctgcccgta gcacgggcca gcgagagccg
      421 cgtgcccccc caaagcacgg gcaaccccga ggatccggga gatcccaccc agtgcgacta
      481 ctggcgctac tgcgccatcg acgggttcct gtgcagctgc tgcggcggca cccagaacgc
      541 ctgcccgccg ggcacggaga tgtcgcctct cacctggatc ggcacctgcc gcaatccggt
      601 ggacggcaag aactacgtga tctcctacaa cgactgctgc ggcaagagca gctgtggacg
      661 ctgcctgtgc aaccgtaacg aggacgaccg gcccgtctac cgaccgagcg ccaacaacga
      721 catcaactgg tgcatgggca cgacctcgag cgtgtacaac tgctccaccg ccgtggtgct
      781 cggcctggcc accgagtaga tcgtgctcaa gctctacgcc ctgttcggcg acgacggggc
      841 cgaatcaccc tttcagctga atcaagcgca ggcactcgag gttcttcagg agcgttttcc
      901 agagctgcgt cgctatcgcc agtttctgag gacgggggag caactcggca atctggtgct
      961 cgcccccttc cgcgcgtcgg ccgagttctg gctggcagag cacgacgacc cgacaccgct
     1021 gatggcggcg ctcgagacgg ccgctgagga cgttcacgag gtcttcgccg tcaacccccg
     1081 tgtgctgatc cgcagcgaag agcacaacat cagcggcgag ccctttcatg acagcggctt
     1141 caaggcgacc ttcctgttcg atcggaagcc gtcgctcagc ctggaggagt tccacgacta
     1201 ctggctgacc catcacggcc ccatcgccgc ccagacccag gacgcggagc gctacgtgca
     1261 gagccacatc aggaccgccc agcgggccta cgacggcgtc acggagctgt tctggcccga
     1321 ctacccgacg gcgctcgcca gcatggcttc agagcagatg accaccgacc aggccaacga
     1381 cgctcctaac ttcgtggatg gcgagagcct ggtcgtgttc ctggcccgcg aggcgaactg
     1441 agaccgacga gttttcggcc gccatcttcc ccttgggtca ggcttgccag aacctccaag
     1501 gtcagaggtg ctgctggatg cttcggacca gcccggccct caccttctcc acttctctga
     1561 gcggtgccga acgcagctcg gccacctcgc ccgccgagta gggcacccag ctcgggggaa
     1621 gctcgccggg gctcatggtg acgagaatcc agttgagcag ctcggccagg gaggcgtcgt
     1681 ccagcgccgc gtttgcggat cccggcacgc ggaccaggaa ctctctgcct cccgggacat
     1741 gcaggaagtt gccgacgaag ccattcatcc gcggcaccgc gtcttccacc ccggaaccat
     1801 cggggagatg gcatccctgg cagttcagca tgtagttcag gcgtgctcgc tccgcgtcga
     1861 ccggcccgtc cccgatggcg tgaggcgcgg cggcgatcag aaaggcaaag cagaccgttc
     1921 gccccttcac tcgacggcaa gccctatcag cacggacgtg gagcagtgat agacggtgct
     1981 ttcggtgcca agacaccagt tgatgtcgtt gttcttatcg ggccggtaca tgggccggtc
     2041 gccctgattg gtgttgcaga agcactgtcc gcagggcgtt tttccgcagc agtcgttgta
     2101 ggagatcacg tagttcttgc cgtcaacggg attgcggcag gtacccaccc aggtgatggg
     2161 cgacatctcc gtgcccggcg ggcaggtgtt gtgagagccg ccacagcagc cgcagagaaa
     2221 accgtcgatg gagcaatgcc gccagtaatc acaggagagc gggtcgccgg tgtcagcaac
     2281 gtcttccagc ttcggctgcc cgccactgga agcccttgcc atcggcagca ggggaaccga
     2341 cgcggcgcca gccagcacgg tgccgatacc cgcaagccac ccgcggcggg acgtctgctg
     2401 ggcgagcccg cgcgtagact tctcagatag tttgtctaac caattcatcg acgatctcca
     2461 ggtctgttca gcgaacgccg tgcacgagga gcggcgtgga catgtccacg ccaagggttc
     2521 tcacatgggt gccgtcagcc tcgtagacgt ctatgaccgg ctcggcgccg cttaccatca
     2581 gctgaggccg ctcgccggtg gagacgctca gcgcgatgcc ccagcgggcc agctcgatct
     2641 tgccgatgcg cttgccgttg gccgcatcga atatccacac ctgatcgccg ccgtccttgt
     2701 gagtaccctc cgcgccgtgt ccgtgcatca gcatatagag ccggccgttg ccgtcgccga
     2761 cggcgggcca gaggccgccg ggacgccagc cctgggcacg ctcggaggcc gtggtcagag
     2821 accaccgttc tcccagcgcg gccgcttcgc cgcgcatatc gatgggagcc acgttgcccg
     2881 taaacgtcgg aaagtaaaac acgccgtcga tctctgccgg ccgggcgaac atggcatcgt
     2941 cgatgacgtc gttgaatggc gccagccgat ccgttcccgc gaccgatccg tcttcgccaa
     3001 gccgggtcgt cagcatcccc ccgtcgctgc acagcgagct gaacccgcgt ttgccggtcg
     3061 ggtaggcaaa catgcatccg ggaacagcta cctcccctag cacctgccga cgctcgagat
     3121 ccaccacggt ggcagactgc gacggcgtga agttgaagac gaccatgtac cgatcgtcgt
     3181 cgagcagggc cacctgataa cggttgggca tgcctgacag gcgcttcggc ggaatgatga
     3241 tttcttccag caccgacagg gtggctggat cgtagatggt gacgacgtcg gtgcgttcgc
     3301 cgccccgacc tccgcgggcg tggaagctct cgatcacgta atactcgttg cgccgggcgc
     3361 tgacgaggaa gctggcgacg aaatcggcgg acatcatgcc gcgcagctgg ccggacaccg
     3421 agtccgcatg gggatcgacg agaaagagct tgccctcccg catgtggttg aaggacagat
     3481 cgtgaatcac cacccagtga tccggattgg gggacggcag ctgggcaacc tcacccagag
     3541 gctcggcctc gaactggtcg gccgcaaggc tcatgccgga tgcagcgagc agcacgctga
     3601 agacgacgaa aacccgcatg gattcctccc actgatccct ggccgaagtg taacccgcgc
     3661 gttggcgatc accaaccaat cggcactacc tgcttccaat cgacagggcg gagacagaaa
     3721 ttcacgaccc cacccattgc cgatcacgcg gcgggacggc actctgccgg cggggatggc
     3781 ggcggctgag ccgtccggca ttccgccacc gagatcagga accttcggga aatcggtgct
     3841 tgcccacgga aaccatcttc gcctgaacgc cttcgttcat ggtttcctcc acgaacatca
     3901 cctcggtacc gggctcgagc ttgtcgaagt ccgcattgac caccgagtta cgatgaaagt
     3961 agatctcgcg accgtcggcc gttccgagaa agccgtagtc ctccgcggcg atccttcgca
     4021 gaacccggcc aacatgatgc tgatcgtgag gcttgacgtg tccgcgcctg acgcggacgt
     4081 agtcctggag ctgccgccga gcggcgtcga aggcatcgcg gatgctcacg tacatgtctt
     4141 catgggcgtg atcgtccccc ggatcacgcg tcaccacgat ctctttgtcc gggacatgga
     4201 tgtcgagccg caggcggtag agattaccct tccggtgccg cccatgagac ttctccacca
     4261 ccacacggca gctcaagatg tggtcgaaaa actcttcgag cttctccgcc ttcgcgacga
     4321 tgtcggcctc cagggcttcg cttttgggga tgtttcgcca ggtaatctgc aagggtatct
     4381 gcaagtcgca actcccatca cgttctacac acgtccaaag atgcgccttt gggttccgat
     4441 tggcgctccg gatttccacg tagttccccg cgggggccgg taggcgtgca gccgaacccg
     4501 gcacgccccg cgaaccccgg ctgccggggt tagcgagaat ccgaggaagc attggagcag
     4561 gtcccccggt agatggccag cagggttccg tcctcgcgcg tgagccggac ctcgcaatgt
     4621 ccgatccgcc gccccgcgcg aacgacttcg gtttcggcgg caatccgttc acccgcggtc
     4681 gccgggcgaa ggtaggttat gcccgcatcg agtgtcaccg gcgccaatcc tgcctcggcc
     4741 agggcatagg cgcacgcggt gtccgccagc gcgaagagca gacccccgtg acagtatcca
     4801 cccgcgttga ccatgtcttc cgccactttc atctcgagcc tgacgccggc gttcgccgac
     4861 aaatcgacgc tgatgttcaa ccgctcgagg acaccgccgt cgcggtcgat gcgatcctga
     4921 atatccaagc tggccccgcc cgcgaatcaa taggcccaat aaacctccaa taccgagccg
     4981 gagtcaacgc gtgagcgttc acccatgggc gtgcgctgta taaaatgccc gctcgctgat
     5041 ccacaggaca caaccgtgac cgccacacat catcgactca tcgtgctcgg atcagggccg
     5101 gccggataca ccgcaacgct gtacgcggcc cgggcgaacc tgcgacccgc gctgattacc
     5161 ggggtggagg tcggcgggca gctgaccacg acgaccgagg tggaaaactg gcccggcggg
     5221 cacgctgagc tccagggccc cgagctgatg gcccagatgg ctgaacacgt ggaacgcttc
     5281 gaggcgaggg tcatcaacga ctacatcacc accgcagacc tcgaggccaa gcccattcgc
     5341 ctccagggag actcccagta cacgtgcgat gccctgatca tcgcgaccgg cgcgtcagcc
     5401 atgtatctcg ggctgccttc ggaggaggcc tttctgggtc gcggcgtgag cgcctgcgcg
     5461 acatgcgacg gtttcttcta tcgcggccag gccgtcgccg tggtcggcgg aggcaacacg
     5521 gccgtggaag aggcgctgta cctgtcgaac atctgcagca aggtctacct cgttcaccgc
     5581 cgcgacgagc tgcgcgcgga gaagatcctt caggaccgcc tgtttgcccg ggagaacatc
     5641 gagccggtat ggaaccacac cctggccgag gtggtcggtg acgattccgg cgtcaccggg
     5701 gtgcgcctgg acggcaccca tggtgccgag ccgatggatc tggacgtgac gggcgtgttc
     5761 atcgccatcg gccaccggcc gaacaccgag atcttcgccg gccagctcga gatggagggc
     5821 ggctacatca ccacccgcag cggtctggac ggcaacgcca ccgcgaccag caccccgggg
     5881 gtattcgcag ccggggacgt ggccgatcac gtctaccgcc aggcgattac ctccgccggg
     5941 ttcggctgca tggccgcgct ggacgccgaa aaatatctgg acgagtcggt ctgagcggca
     6001 ggcccggtat catcccacca cggggtcccg cagaagctcg tcgatgacgg catccaccac
     6061 cgcccgcaac gagccggtcg ccgcaaacct acgccgctgg cgttccgatg ggagccctgc
     6121 ggagatcgct cgtgccagcc tggaaaggtg gtccgtgtct ccgaaccgcg cagccgtgtc
     6181 ggctaccaga tcgagcgtct gacgtgcgac ctcgcgcagc gtcatcacat cgccgttctc
     6241 gttgacggcc acccgggcgt cgaggccgaa acgagaggcc aggatccgcg gccggaaagc
     6301 ggcaaaacgg gtgtcgtgac cctgccagaa tggcgagctc gccgagagcg ccagcatcag
     6361 cggcaggtaa ggggccagct gcctggcgag ccatagggcc tcgtccccat cgatgacgct
     6421 caggtgaacg tgggtagaaa aggtgatctg acgatgcccg agcagccccg acgtccgctc
     6481 catggattcg tatcgggggc ctggcgtgat ggccgcaagc ctggtggaga acggatgcgt
     6541 ccccgcgccg accagccgca ttcccaacgt ccggcagctt tcggcaggtc gggcgacgct
     6601 ttggccgagg tccttgccga gatcgctcga cgcgacctgc atgaggagaa cgcagccggg
     6661 aatccgttcc aggtagtagg agaaatcctc agcacccatg ctcgggtgat caagctgcat
     6721 caggccggcg tcgccaacca cgtcgcgagc ggcccgaaag gcaagctcgg cctcccggcg
     6781 gctgttgatc accggcgggt agcccgtgtc gatggatacg tccagggacg cgttgtgcag
     6841 ctcgccgcac gcccgggccg tgcacgtcga tggcaaatcg gtccgattgg gccgtgatcg
     6901 taccggcgtt caccgccgaa tcgacccatt gcgtcacggc ccgtttcctt ccccaaccga
     6961 cgctgccgca tcgatccgct cgagcagctg gctgagccgg cgcaacgcct cgcccgccgc
     7021 ttcgaagcgc ttgtctccca gcgcctcgag atagctttcg aaagccattc gcgcctcgat
     7081 ggccaaccct ttgacggacc gagacggcgt ctccccgacc agttgccggc ccgtgaagag
     7141 gcccagcagc gcctcatcca gggtctcggc gtagctcaga ttgtctccct gcatcacggc
     7201 aaccagtcgc agctcaggat acgcggcggt ctcggcgcga agatagatgg gctcgacgta
     7261 caggagcgag tcgtcgatgg gtattgccag gacgttcccg cgtatcacct cggagccctg
     7321 ctggtcccag agcgtcagct gccccgacag gaacgagtcc tgatcgatct tggtttcgac
     7381 ctgctgaggc ccgagcaccc gccgttcctt gggaaacttg taagcgatga agcgcccgta
     7441 gttctcgcca tcggatagcg cggcaaccca gcctatgagc acctgccggt ttttgggcgt
     7501 gaacggcagg atgagcacga attccgcttc gtccgagcct ggcagctccc acatcacgta
     7561 atagggctcc accggcgtga tgtcgtgata gtgcttctcg gtggctcgca cccaaacgtc
     7621 ttcctggttg tagaagacgt cgggatcgga catgtggtag cgggtgaaga tgagcccctg
     7681 agcgagcagg aagtcctggg gataacgcac gtgggcgacc aggctggccg gcatttcgga
     7741 gcggggcttg aacaggcccg gcagcgcgcg ctgccacacc tgaatgatga ggtcttcttc
     7801 gtcgaacacg gataagtcca cgctgccgtc gtaggcgtcg atgacggcct tgacagagtt
     7861 acgcacgtag ttggcgccgt cgagccgctg caccgtccgc acccgcgcct gaccctcgtc
     7921 ccgactcagc ccgaagaact cttcgctgtc gaaggcctgg ctgtaagggt agtagctcga
     7981 actcgtgtag gcgtccacga tccacttcag acgcccgtcc acggcaacga tgtaggggtc
     8041 ctcgtcgaag accagaaacg gggcgagccg ttgcacccgc tccggcacct gacggtaaaa
     8101 caagacccgg ctctccggcg tggggtaact cgacaacagg aaaaccgtgc cgtcgaactt
     8161 ccagccgaac aggaacttgc gccagaagct cgatagcacc acgcccccag tacccgagta
     8221 gcgggtgtag atgttggcat ccccgcttgg ataatcgaac tcgggctcga gggtattggt
     8281 gaccaccggc tctatggtga gttcgccgta gtagatctcc ggccgggtta cctccagctc
     8341 cggctggtcg gtgaccggcg gaatgtcctt gaccagcagg ttgggcaacc cttccggcgt
     8401 gaaatcgctc accggggcca gggtgtaacc gtaaccgtgg gtgtacttga agcgctggtt
     8461 gacgaacgtg cggctctgtt cagggaggtt acgttgagcc agttcccgtg ccgccaccat
     8521 gacctgacga tagcggtcac ccagccggta gcggtcgatg tcgacatcta cgaactcgta
     8581 gtaaaggcgt atttcctgaa actgcttgta gacggcgtcg agagcacgcc aatcccacag
     8641 ccgtacctcc gaaatgagat ggcggttgtc ggccagagac tccgggccga gcgccgcgtc
     8701 ggcggaaaac tgccgctcct ccacccgatc gagcttgaaa ccacgccgtg tgaattcgat
     8761 gttgttctcg atgtagggct tctcgaaggt gatctcgttg ggcttgacca ccagccactg
     8821 ggtcagcgca gggagcagaa ccaccagcag cagccatgcg ccaccgatca ccgcccacgc
     8881 accgaccacg gcgaccacgt tgacgggcgc gatggcctcc cagcgcgcga ccagccgcgc
     8941 catgaagcca cgaaaaccct cgaagagcgg cagcatgccg gccaccagga aaatggcgcc
     9001 catcagaatc agggcaggca gccgaacgtg aacgtcggtc cagcccgggc ccatcaccac
     9061 gccgagcttc gagtacagca gatgcagaac gctcaagagc tggccaaagg ccagcacgac
     9121 ggtcagcgtc aggctggcgc cgggaatgag gagccgcccc ttcggcaccg cgaacgccgg
     9181 ccccggcatc accaccccgt cctgctcgcg caacgccagg accgtgatga gcgagatcag
     9241 agcgacgaag aggcccagcc agaagacgcc gtcaagcagg ggcagaacga aaaggtagaa
     9301 ccccgtgtcc agcccgagca gcgggtcgac gatcccggtt tccattcgat tgagaaagag
     9361 cagcacgggc tgccaggatg caagccccca gatcactccg gccgcggcgc aagcgaaggc
     9421 cacgccgcgt ctgaggtaga tgcccgcgcc ccgcgtcagc accagtcctg tgaggcccgc
     9481 cgcggcgccg cccagcagag gcaccaaaag gcgaacccag acgaaggtcc agaaccgatc
     9541 ctgatagccg agcgagtcga accacagcat ctcggcccag aaagccatca gtaacatcag
     9601 cccgaacgcg gaaccgacga tggctgcacc gccccagacc tgcactttgc ggtgatgccg
     9661 actgccggaa tgagccagcc aggctccaca gcccagcaga gcaacgaaaa gcaggacgta
     9721 catggttcac acccttcttg ccgaccgttc cagtctggca ggcgggttcg cctgggcaac
     9781 cgtggttact acctagacct taaggccgtg tcgggcgccg ttagcccttc acgcagagca
     9841 cgggaaccag ccgcgccagc gccgggtggc cgcgcggcgg ctcatggccc gacccgcccc
     9901 atgacaggcg gaggagtagg cgcggcgctc gctttccttc gtcccgcgca gaacgtaaga
     9961 ctcggttgcc atgctgccgc cgatcagcac cggctgccgc acggacctca gggggtcggg
    10021 caggctcggg tgaccggggc caaacgctcg cgtgacaccc ttgcggtgaa cgaacagctc
    10081 ccgggtctcg tcgccaaccc ggtgggtttc caccttgcac gtgttgtgag agacgtcgaa
    10141 gagcatctcg agccgcgctg ccgggaagca tctggcgaac accccgcgaa cccccatgtc
    10201 ggccgcatga tcgaagtggc tccaactcgc gatggcccga tcgtcgctca actgtcagcc
    10261 ccatgattcg cacggttgcc gcgcacaaca aatgacctga tcggcgacga tttccgacgc
    10321 taccgcgtag ccgaaagctc gaacataggg gatgtccgag gtggcgatcc aaggtggtca
    10381 ggtgcgccgc aaacgctgga tcaggctgga agtatcccaa cggccgccac cgtagctctg
    10441 cacgtcggca taaaactggt ccacgagtgc cgtgagcggc aaacgggcct gcagccgacg
    10501 tccggcctga agcgcgatgg cgagatcctt gcgcatccag tcaacggcga aaccgaaatc
    10561 gaactcgccc cgggccatgg tggcgtgccg gttctccatc tgccaggact gggcggcgcc
    10621 cttggagatc acgtccacca ccgattcgat gtccagcccg gcctgatcgg caaagtgcag
    10681 cccctccgcc agcccctgca gaaccccggc aatgcagatc tggttgacca tcttggtgag
    10741 ctgcccggtg ccagcgggac ccatgcgacg gaccgatttg ccgtaggctt ccatgacggg
    10801 cagagcgctg tcgaagatcg tctgctcccc gcccaccatc accgtcagct gtccgttctc
    10861 ggctcccgcc tgacctccgg agacgggcgc gtccagaaac ccgcaaccgc gctcgccggc
    10921 ggcagaagcc agctcctcgg ccagctccgc ggagaccgtg gtgtggtcga tcagcaccga
    10981 gccgtcggcc agcccgtcga gcacaccctc gggcccgtag acgacggcgc gcacgtcgtc
    11041 gtcgttgccc acacaaaggc agaccacgtc cgcccccgac gcagcctccc gcggggtttc
    11101 gacccgggac ccgccatgct cggttaccca tcgctcggac ttggcgccgg ttcggttgta
    11161 cacgcgcacg ttgtagcccg cgcgggccag gtgtccggcc atgggataac ccatgacgcc
    11221 gaggccaacg aaacccacat tgccgatccc gctcaccatc cacctcctct ggcttggagc
    11281 cacactggct gcctacacgt catcgtagtc gagcaccagc tcgctgctgg tcgggcggga
    11341 ctgacaggcc agcacgtacc cgttctcgat ttcccagtct tccagcgaaa aattctcagc
    11401 catctccgcc gcaccttcca cgacgcgggt acgacacgtg gagcacacgc cggcgcaaca
    11461 ggagtaaggc agatcgatcc cggaatccag cgcggcatcc aggatggact cgtcgtccgg
    11521 gctcatggtg aacgatcggc gacggccgtc catgatcacg atgatctccg ccgcgccacc
    11581 cgtccgtgga ggcggctccc cgtcgcgatc cgccgcagcc acgctttcca ccgtgaagtg
    11641 ctcgctgtga atccggtccg gggcaacgcc cagcttgccc agggcggcgc cgaccgccgc
    11701 gatcatgtcg ccggggccgc acaggaaaaa ctcgtccgtc gcggcggggt cgaagagctt
    11761 gccggcactt gcttggacct tggccgcgtc gatgcggcca ttgaacatct cggcctcgct
    11821 cggctcacgg ctcatcacga agtgcagggc gagccggccg aggtagcgat ccttgaggcc
    11881 cagcaactcg tcgagaaaca tggcccggcc cgcgctgcgg ttgccgtaga acaccgtcca
    11941 cctgcagccc ggctcttcgg cgagcttggc gcgcacgagc gcgagcaccg gggtgattcc
    12001 acacccggcc gcgaaagccg cgtagcggcg cttcgccggt ccctggcatt gactggcgaa
    12061 gctgccgagc ggcggcatca ccgaaaggcg cgctcccggc gtcagccggg aaagatacga
    12121 cgacatccgt ccgtcagcat gcaggcgaac cccgatacgc agcggaaact cgcccgggct
    12181 gctgaccagg gagtaggatc gtctgacctc ctcgccgtcg atgtcggctt tcaacacgac
    12241 gtgttgcccg gccgcaccgc taaaggcttc tcgcagctcc ggcggcacga cgatctgcag
    12301 gcaaagggct tcctcgcctg cagacgtgca cccagccact tcgaggtcgt gaaatttcag
    12361 caactcagga aatctccgcc gtcagtgaca cttgaagtag tcgaaaggct ctcgacagga
    12421 aaggcacctg aagagcgcct tgcagggagt ggagccgaac tggctgatca tctcgatttt
    12481 acgaccgccg caatgcggac aggactccgg ctcgtcgggc gaggacgggt tcggcggcgc
    12541 tatgccgtac tccaggagct tgcggcgacc ctcggcgctg atccaggcgg cggaccaggg
    12601 cggtgagagc acctcctcga tccttgcatt cgggtagccg ctctgggcaa gggcgctgcg
    12661 aatggatgcg cgtatcacct ccgtggccgg gcaccccgag taggtcggcg tgatccctac
    12721 ctgcggaccc ggcatgcgaa cgtagcggac gatgcccagg tccacgacgc tgagcaccgg
    12781 gatctcggga tcgggcacac cacgcaagac cgcccagacc gagtcttcac cggcttccgc
    12841 tgcgatgatg cgcaccggct gctcgtctac cacgatgccc ccgggtaagt ccgctgcagg
    12901 acctgcatct ctgccagcag gtg
//
LOCUS       DBNHLM010000132         4093 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_735992, whole genome shotgun sequence.
ACCESSION   DBNHLM010000132 DBNHLM010000000
VERSION     DBNHLM010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_735992"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="AC16QF_06190"
     CDS             complement(<1..213)
                     /locus_tag="AC16QF_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR6448179.1"
                     /translation="MVAEAKPVGPKRFRESYGRYLEDFAVGDVYEHRPGRTITETDNT
                     WFTLLTMNQHPLHFDKEYGANSEFGRV"
     gene            complement(316..1186)
                     /locus_tag="AC16QF_06195"
                     /pseudo
     CDS             complement(316..1186)
                     /locus_tag="AC16QF_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003200383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
     gene            complement(1183..2265)
                     /gene="hppD"
                     /locus_tag="AC16QF_06200"
     CDS             complement(1183..2265)
                     /gene="hppD"
                     /locus_tag="AC16QF_06200"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - L-tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HIR6448180.1"
                     /translation="MTNRVDNPMGLDGFEFVEFSATQPGQIEPVFAMLGCQEEARHRS
                     KDVRLWRQGGINLVVNYEPRSVAWYYAEEHGPSACGLAFRVRDAHVAYKRALELGAQP
                     VEIPTGPMELRLPAIRGIGGAPLYLIDRYRPGSSIYDIDFEYLDAVDRFPEGIGLEII
                     DHLTHNVYRGRMDHWARFYETLFNFQEIRHFDIRGEQTGLSSRAMTAPDGKIRIPLNE
                     EARGAGDGQVEEFLLQYNGEGIQHIAFACQDLPSKLDRLAAAGVPLMEPPPDAYYDML
                     EDRLPGHGEPVGELKQRGILLDGSTGEGEPRLLLQIFSEPLIGPIFFEFIERKRDEGF
                     GEGNFQALFESMERDQIRRGAIEGPS"
     gene            complement(2315..3208)
                     /locus_tag="AC16QF_06205"
     CDS             complement(2315..3208)
                     /locus_tag="AC16QF_06205"
                     /inference="COORDINATES: protein motif:HMM:NF003784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HIR6448181.1"
                     /translation="MSNFDDQLLKIESANGFIAALDQSGGSTPKALGLYGVDSSAWSN
                     DEEMFEIVHQMRARIMTSSSFTGERILAAILFENTMDREVEGRPTADYLWDVKNVVPI
                     LKVDKGLADEAQGVQVMKPMPDLDDLLTRAKAKGIFGTKMRSVIKQADAGGIKTVVDQ
                     QFEIGRRIVAAGFVPIIEPEVDIHCPDKAGAEQILKGALLAGLDALRPEERVMFKLTL
                     PEADNFYAECIDHPNLVRMVALSGGYSREEANARLARNNRMVASFSRALTEGLSAQQT
                     QAEFDAALDASIQSIFEASLT"
     gene            complement(3248..4033)
                     /locus_tag="AC16QF_06210"
     CDS             complement(3248..4033)
                     /locus_tag="AC16QF_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6448182.1"
                     /translation="MSDEVVLYEESENVGVVTLNRPEAMNSLTYQSYLELEDRVRECS
                     ARVLVITGQGRAFCAGDDVKHILGSSQPAPPEFAERARRSGGLTPAADALLHTNIPVI
                     AAVNGPAVGWGMELALMADIRVASENAKFGELFVIRGLCCDAPGLGRLAQLVGRERAA
                     ELLFTGEVIDAETARSIGLVSRVVPQEDLMATALDIARRIASNPPLAVQALKAGLRRT
                     LEPDWRETGRWASEQIAALRKTEDSKEGVAAFLEKRPPVYVGR"
BASE COUNT          731 a         1367 c         1215 g          780 t
ORIGIN      
        1 caccctcccg aactcggaat tcgccccgta ctccttgtcg aaatgaagcg gatgctggtt
       61 catggtcaac agcgtgaacc aggtgttgtc agtttcagtg atcgtccgtc ccggtcgatg
      121 ttcgtacacg tcgcccacgg cgaaatcttc cagatatcgc ccgtaggact ctcggaaccg
      181 cttaggcccc accggctttg cctctgccac catgtttgcc acctcctcgg ttgcgtcgcc
      241 cgctcccgaa gcgcgcgttt gcccggcctc gcttcgggac aaaagtagaa cacattcacg
      301 accccgaatg acaattcagc cggtcccgac caccacccgc tggtcgatgg agccgaagat
      361 gctggagccg tcccggtcga gcatttcgat ggtgacccga tcccccgcct gcaggaagga
      421 cgtctgcggc gccccatcgc gaatggtctc gatcatgcgc agctctgcaa tgcacgagta
      481 gcccacgccg ccggccgcga ccgaagagcc gcccgttgac atagctcaac agggggcgtt
      541 gcaccttgtt ctcacgccag tcgccgcaaa gctcgtcggg agacacggcc accggcgaaa
      601 acgccgtggc cggcttggat tgatagaagc cgaagccttt gtccagctcc ccgggtatca
      661 gcccgcgcag cgagacgtca ttgacgagca tgagaagctt tatgtggtca ccggcatcga
      721 ccgtcgaggt gcccatctcg acgtcatcga cgatgacggc gatctcggcc tcgaaatcga
      781 gcccccactc gctgctcctc acgacgatgt catcggtggg ccccagcatg acgtcggatc
      841 cgccctgata aatcagaggt tcacgccaga agctcgcggg catgtcggca ccccgcgcgc
      901 gacggaccag ctccacgtga ttgacgtagg cactgccgtc cgcccattgg taagcgcgcg
      961 gcagcggcgc cgcgcaacgt ttggggacga atggaaacga ggcttccagg gcaccgtcgt
     1021 tgagtcgttc gtagacggag gcaagctttt cagcaaccgt accccatcgg tcgagggcgc
     1081 cctgcaacgt gggagcgatt tcccccacgc gaaccgcccg gctcagatcc cgcgagacga
     1141 cgaggagctc accatcccga tcctcccctc gcgttgcaag cttcacgatg gtccttcgat
     1201 ggccccgcga cgaatctgat cccgttccat ggattcgaac agcgcctgga agttgccttc
     1261 tccgaaccct tcgtcgcgtt tgcgctcgat gaactcgaaa aatatcgggc cgatcaacgg
     1321 ctcggagaag atctgcagca gcagcctcgg ctcaccctcc cccgtactcc catccaacag
     1381 gattccacgc tgcttgagtt cgcccacggg ctccccgtga cccggaaggc gatcttccag
     1441 catgtcgtag taggcgtccg gcggaggctc catcaatggc accccggccg ctgccagacg
     1501 atccagcttc gacggcaggt cctggcaggc aaaagcgatg tgctggattc cctcaccgtt
     1561 gtactgcaga agaaactctt ccacctgccc gtcgccggct ccacgggctt cctcattgag
     1621 cggtatccgg atcttgccgt ccggggcggt catggcccgg gagctcaagc ccgtctgctc
     1681 accccgaatg tcgaaatgtc ggatctcctg aaagttgaaa agcgtttcgt agaaacgggc
     1741 ccaatgatcc atccgccccc ggtagacgtt atgcgtaagg tgatcgatga tttcgagacc
     1801 gataccctcg ggaaaccgat ccactgcgtc gagatattcg aagtcgatat cgtagatgct
     1861 gctgcccggc cgatagcgat cgatcaggta cagcggtgcg ccgccgattc cgcggattgc
     1921 cggcaggcgc agctccatcg ggcctgtggg aatctcgacg ggctgggcgc cgagctccag
     1981 cgcccgcttg taggcaacgt gggcatcgcg gaccctgaat gcgagaccgc atgccgaagg
     2041 cccgtgctcc tcggcgtagt accaggcaac gcttcgcggc tcgtagttga cgacgaggtt
     2101 gatcccgccc tgccgccaga ggcggacgtc cttggagcga tgccgggcct cttcttgaca
     2161 cccgagcatc gcgaagaccg gctcgatctg acccggctgc gttgcggaaa actcgacgaa
     2221 ctcgaaaccg tcgaggccca tgggattgtc aacgcggttc gtcatcctta tcgcatcgcc
     2281 gtgacgggtt caggcagctg gccgcgctgg cgcttcaggt tagagaggcc tcgaagatgc
     2341 tttgaatgga ggcatcgagc gccgcgtcga attctgcctg ggtctgctgc gcggaaagtc
     2401 cctccgtcag cgcccgcgag aagctggcga ccatccggtt gttgcgggcg agccgcgcat
     2461 tggcctcctc ccgtgagtag ccgccggaga gcgccaccat ccgcaccaga ttcggatgat
     2521 cgatgcactc cgcgtaaaag ttgtccgcct cgggcaaggt gagcttgaac attaccctct
     2581 cctcgggcct cagggcatcc aggccggcca agagagcgcc tttgagaatc tgctcggctc
     2641 cagccttgtc cggacagtga atgtccacct cgggctcgat gatgggcacg aatcccgcgg
     2701 cgacgattcg gcgaccgatc tcgaactgct gatccacgac ggtcttgatc cctcctgcgt
     2761 ccgcctgctt gatcaccgag cgcatcttcg tgccgaagat gccctttgct tttgctctgg
     2821 taagcagatc gtccaggtcc ggcatgggct tcatcacctg aacgccctgc gcctcgtccg
     2881 ccagcccctt gtcgactttg aggatgggta ccacgttctt cacgtcccag aggtagtcgg
     2941 ccgtggggcg gccctccacc tcgcgatcca tcgtgttctc gaaaaggatg gccgcgagga
     3001 tccgctcgcc ggtgaagctc gagctcgtca tgatgcgcgc gcgcatctga tgcacgatct
     3061 cgaacatctc ctcgtcgttg gaccacgcgc ttgaatcgac cccatacaga cccagggcct
     3121 tgggcgtgct gccgccgctc tggtccagcg cggcgatgaa tccgtttgct gactcgatct
     3181 tcaggagttg gtcgtcgaaa ttactcactt gtcatgctca cttatcggtt ggttactccg
     3241 gaacgggtca acgacccacg tagacgggcg ggcgcttttc gaggaatgcc gcgacgccct
     3301 ccttcgagtc ctcagtcttt ctgagcgcgg cgatctgctc gcttgcccaa cgaccggttt
     3361 cccgccagtc gggctcgagg gttcggcgca acccggcctt caaggcctgc acggccagcg
     3421 gcggattgga ggctattctg cgggcgatgt ccagggcggt cgccatgaga tcttcctggg
     3481 gcactacccg ggagaccagc ccgatgcttc gcgcggtctc ggcgtcgatg acctcgcctg
     3541 tgaacagcag ctccgcggcc ctttcgcggc caacgagctg ggcgagacgg ccgagaccgg
     3601 gggcatcgca gcacaaccct cgaattacga acagctcacc gaacttggcg ttctccgagg
     3661 ccacccgaat gtcagccatc aacgcgagtt ccatgcccca gcccacggcc ggcccgttga
     3721 ccgcggcgat gacgggaatg ttggtgtgga gcaacgcgtc cgccgcgggg gtcagaccac
     3781 cactgcggcg ggcgcgctcc gcgaactcgg gcggcgccgg ctggctgctg ccgaggatgt
     3841 gcttgacgtc gtcaccggca cagaacgcgc gcccctgccc ggtgatgacg agcacccggg
     3901 ccgagcactc gcgcacccgg tcctcgagct cgagataaga ctggtaggta agagaattca
     3961 tggcttccgg gcgattgagc gtgacgacgc cgacgttttc gctctcctcg tacagcacta
     4021 cctcgtcact catgttctca gcccctcgtt cagtcgttgc gacggtcgac ccgctcgttt
     4081 cggcgtcgca tcc
//
LOCUS       DBNHLM010000133         8214 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_437517, whole genome shotgun sequence.
ACCESSION   DBNHLM010000133 DBNHLM010000000
VERSION     DBNHLM010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_437517"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1835
                     /gene="metG"
                     /locus_tag="AC16QF_06215"
     CDS             <1..1835
                     /gene="metG"
                     /locus_tag="AC16QF_06215"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR6448183.1"
                     /translation="GVEPERLIERIRAEHARDFQGFLVAHDNYYSTHSQENRHYAELI
                     YQRLKDGGLIFTREVAQLYDPQRKLFLADRFVKGRCPRCDAEDQYGDNCEACGATYDA
                     TELKDPRSLISGAVPVLERSEHHFFDLPQYTEMLKAWTAGGAVQPEVQNKLAEWLDAG
                     LKPWDISRDAPYFGFLIPGTQDKYFYVWMDAPIGYMASFQNYVSERKDVDFDTYWEPE
                     GDAEVHHFIGKDIVNFHALFWPAVLEGAGFRKPTRLHVHGFVTVEGRKMSKSRGTFVN
                     ASTYLEHLNPEFLRYYYATKLNGTIDDMDINLDDFVQRVNSDLVGKVVNIASRCAGFI
                     TKQFDGRLAGTLHDAELQGRFVDAAAPIAELFEAGDTSKAVREITALADQANQYIAHH
                     APWSMIKEAGREAEVQAVCSQGINLFRILAIYLKPILPRMASEAEAFLEIEPLAWKDV
                     DAPLLDHPIGAFRPLFTRMERKAVDRMIEAGREAGSEPVPDSAPSSEAPAESQQISID
                     DFARIDLRVAKISAAEPVAGADKLLRLTLDLGDSNRQVFAGIKSAYEAQSLQGRLCVV
                     VANLAPRKMRFGVSEGMVLAAGPGGKDIFLLSPDSGAEPGMIVR"
     gene            1842..2420
                     /gene="rsxA"
                     /locus_tag="AC16QF_06220"
     CDS             1842..2420
                     /gene="rsxA"
                     /locus_tag="AC16QF_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HIR6448184.1"
                     /translation="MAELALIVVGALLVNNFVLAQFLGLCPFIGVSGNYDTAVAMGFA
                     TTFVLTLAAAVSYMAYHWLLLPLGLEFLRVIVFIVVIAALVQLTEVFLRNTSPVLHNA
                     LGIYLPLITTNCAVLGVALLAINHSLSLLGTVAYAIGAAVGFTMVLVVFAAMRERLAH
                     AEVPAAFRGAPITLISAGILSLAFLGFAGLGN"
     gene            2440..3354
                     /locus_tag="AC16QF_06225"
     CDS             2440..3354
                     /locus_tag="AC16QF_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HIR6448185.1"
                     /translation="MLTALVLTAVLTAVVAALGAALVWADRRYPPDTRTLVESIDGLL
                     PQTQCAQCGYPGCRPYAEAVAAGAAINLCPPGGPETHRALARLLGRSEEDPPQEASKL
                     KARIREDGCIGCYLCVEACPVDAIVGAPRYLHTVLEAACTGCELCVPACPVDCIDMTP
                     AREASPISHQPRRRWPDRQSSPCIRCGACQPACPVGLSPQELLWYAGGSSSGRPTGQA
                     AAATRGLDRCIECGLCNPVCPSNIDLVGVFRQARRAQAARHEAERDAADAKARHARRT
                     ERLEQTTAKRAEDREQRIARRSGRQWRA"
     gene            3342..4253
                     /locus_tag="AC16QF_06230"
     CDS             3342..4253
                     /locus_tag="AC16QF_06230"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR6448186.1"
                     /translation="MAGLSSRGIMMAVCLALLPGTATMIYFYGVGVVHNLLLAVPVGL
                     AVEAICLQLQQRSVGRTLADGSTVLTCVLIALALPPGAPFEILSIAVVAAVALAKHLY
                     GGLGSNLFNPALVGYAVVLVSFPVAMASWPIPMDGETGATALVSMKYREGLTVAEAWQ
                     GARGFGAIGGYGWEWINLGFLLGGAVLVWRRLAAWRVPATILATIAVLAAAFWDGGSS
                     TSSGSPLFHWFTGSTMLTVFFFATDPVTHPVSPRGQIVFGIVVGTATFLIRAFGNYPD
                     GAAFAILLGNALTPYLDKRLQEASANA"
     gene            4246..4734
                     /locus_tag="AC16QF_06235"
     CDS             4246..4734
                     /locus_tag="AC16QF_06235"
                     /inference="COORDINATES: protein motif:HMM:NF016121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR6448187.1"
                     /translation="MHKLVLIAAACAMLLALTDAWTADRIARNARSHELSQITDLAGA
                     APPADTTWNEDVWDFCSGTKLARSSAAGYGGPIRLVVGVSQGRILGIRVTAHQETPGL
                     ADFLREPKRGWLAALNGRTGPETAAVDAVAGATITSEAVVRAVRDAMDRAQDASAEGC
                     RA"
     gene            4731..5396
                     /gene="rsxE"
                     /locus_tag="AC16QF_06240"
     CDS             4731..5396
                     /gene="rsxE"
                     /locus_tag="AC16QF_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HIR6448188.1"
                     /translation="MSVAKPFRNAAIASNPAWAQLLGLCPLLAVSTSLVNALALGAAS
                     AFVLLGSNITIAAIRRWIPELARLPSFVLVIATFTTCAMLLMQAYAFELYLRIALFVQ
                     IIVTNCMILGRVEAFASRQPVGRAFLDAAGTACGFALALLAMGAARELIGHGSLMAGM
                     EQLIPGSESWRLNLSESGRGLLLASLPPGAFIIAGLLLGAGNAILRRPNPAPSGSAGE
                     NSA"
     gene            5393..6034
                     /gene="nth"
                     /locus_tag="AC16QF_06245"
     CDS             5393..6034
                     /gene="nth"
                     /locus_tag="AC16QF_06245"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR6448189.1"
                     /translation="MNNDKRRAIFERLRAANPAPTTELDYRNPFELLIAVILSAQATD
                     VSVNKATGPLFEAAGTPTEMKALGLRKLKSYIKTIGLFNTKAANILKTCEMLLTQHGG
                     EVPEDRQALEALPGVGRKTANVVLNTAFGHPTIAVDTHIFRVCNRTRIAPGKNVNQVE
                     QKLLKVVPDEFKRDAHHWLILHGRYTCIARKPRCGSCVIEDLCEFRQKTDLEG"
     gene            complement(6067..6633)
                     /locus_tag="AC16QF_06250"
     CDS             complement(6067..6633)
                     /locus_tag="AC16QF_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448190.1"
                     /translation="MNNYQVLFSGEVSPQANRDIVRAKLACELDIDDRKAAQLFSGRT
                     VVIKSQLSHRQAMELQERLASLGAICRVKDLTPKTRNNVADFRVDRSGKSDATLRDIT
                     AAHTECPRCGHMQLEAAHCTRCGVDIAAAVRQRQKEDLIIEKKIRELRAKQDGLLGRA
                     GAAPGKALQGAASSTVKTRGIGWFRKSS"
     gene            complement(6637..7848)
                     /locus_tag="AC16QF_06255"
     CDS             complement(6637..7848)
                     /locus_tag="AC16QF_06255"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR6448191.1"
                     /translation="MKIVLAYSGGLDTSVICRWLQETYGAEVVTFTADIGQGEEVEPA
                     RAKAQAMGVQEIFIEDLRDEFVRDYVYPMFRANTLYEGEYLLGTSIARPLIAKRLVEI
                     AAETGAEAVAHGATGKGNDQVRFELGAYALNPDIRVIAPWREWDLNSREALMAFCEQH
                     QIPVDYQRGAKSPYSMDANLLHISYEGDNLEDPWQEADADMWRWTVAPEEAPDEAQLV
                     EIDFLQGDPVAVDGEKLGPADLLARLNSLGGAHGIGRSDIVENRFVGMKSRGCYETPG
                     GTLLLKAHRAIESLTLDREVTHLKDELMPRYATLIYNGYWWSPERKVLQALIDESQKP
                     VSGRVRLKLYKGAVSVVGRMSQEESLYDANVVTFEDDGGAYHQADAAGFIRLNALRLR
                     LGARRGRKLSE"
     gene            8017..>8214
                     /locus_tag="AC16QF_06260"
     CDS             8017..>8214
                     /locus_tag="AC16QF_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013122.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HIR6448192.1"
                     /translation="MADRFRGFLPVVVDLETGGFDPGSHAILEIAAVQLRFDRGRIAP
                     DRKWHQAVEPFPGSRVEAASLK"
BASE COUNT         1341 a         2807 c         2629 g         1437 t
ORIGIN      
        1 agggagtcga gcccgaacgg ctcatcgagc ggatccgcgc cgagcatgcc agggacttcc
       61 agggctttct cgtggcccac gacaactact actccaccca ttcccaggaa aaccgccatt
      121 acgccgagct catctaccag cgcctgaagg acggcggcct gatcttcacc cgggaggtgg
      181 cccagctcta cgacccccag cggaagctgt ttctcgccga ccgtttcgtc aagggccgct
      241 gcccgcgctg cgacgccgag gatcagtacg gcgacaactg cgaagcgtgc ggcgcaacct
      301 acgacgccac cgagctgaag gatccgcgct cgttgatctc cggggcggtc cccgtgctgg
      361 agcgctcaga gcaccacttc ttcgatcttc cccagtacac ggaaatgctg aaagcgtgga
      421 cggccggcgg cgcggtgcag cccgaggtgc agaacaagct ggccgagtgg ctcgacgcgg
      481 gcttgaagcc ctgggacatt tcccgggacg cgccgtactt cggctttctg attccaggga
      541 ctcaggacaa gtacttctac gtgtggatgg acgcgcccat cggctacatg gcgagcttcc
      601 agaattacgt ctccgagcgc aaagacgtcg atttcgacac gtactgggag ccggagggcg
      661 acgccgaggt acatcatttc atcggcaagg acatcgtcaa ctttcacgcg ctgttctggc
      721 cggcggtgct ggagggcgcc gggttccgca agcccacccg tctgcacgtt cacggctttg
      781 tcaccgtcga agggcgcaag atgtcgaaat cgcgaggcac gttcgtcaac gcgtccacct
      841 acctcgagca cctcaacccc gagttcctgc gctactacta cgcgaccaag ctgaacggaa
      901 ccatcgacga catggacatc aatctggacg acttcgtcca gcgcgtaaac tccgatctgg
      961 tcggtaaggt cgtcaacatc gccagccgct gtgccgggtt catcaccaag cagttcgacg
     1021 ggcgcctggc gggcacgctg cacgacgccg agctgcaggg gcggttcgtc gacgccgccg
     1081 cgcccatcgc cgagctcttc gaagccggcg acaccagcaa ggccgtgagg gaaatcacgg
     1141 cgctggccga ccaggcgaac cagtacatcg cccaccacgc gccctggtcc atgatcaagg
     1201 aagcgggccg agaagccgag gtccaggcgg tgtgcagcca gggcatcaac ctgttccgca
     1261 tcctggcgat ctacctcaag ccgatcctgc cgcggatggc gagcgaagcc gaagccttcc
     1321 tcgagatcga accgctcgcc tggaaggatg tggatgcgcc tctgctcgat cacccgatcg
     1381 gcgcgttcag gcctctgttc acgagaatgg agaggaaggc cgtggaccgg atgatcgagg
     1441 cgggccggga ggcgggctcc gagcccgtcc cggactccgc cccatccagc gaggcgcccg
     1501 ccgaatcaca gcagatcagc atcgacgact ttgccaggat cgacctgaga gtggcgaaga
     1561 tcagcgccgc ggagcccgtg gcaggcgccg acaagcttct gcggctgact ctggatctcg
     1621 gcgattccaa ccgccaggtc ttcgccggta tcaagtctgc ctacgaagca cagagtctgc
     1681 agggcaggct gtgcgtcgtg gttgccaacc tcgctccgcg caagatgcgc ttcggggtat
     1741 ccgaggggat ggtgctcgcc gccggcccgg gcggaaagga catcttcctg ctgagcccgg
     1801 actcgggcgc cgagccgggc atgatcgtgc gctgagccgc catggctgag ctcgctctca
     1861 ttgtcgtcgg cgctctgctc gtcaacaatt tcgtgctggc ccagttcctc ggcctgtgcc
     1921 cgttcatcgg cgtgtccggc aactacgaca cggcggtggc catggggttc gccaccacct
     1981 tcgtgctgac cctggcggcc gccgtgagct acatggccta tcactggctg ctgctgcctt
     2041 tggggctgga gtttctgcgg gtcatcgtgt tcatcgtggt catcgctgcc ctcgtccagc
     2101 tgacggaggt ttttctgcgc aatacgagcc ccgttctgca caacgccctg ggcatctacc
     2161 tgccgctgat caccacgaac tgcgcggttc tgggcgtcgc cctgctggcc atcaaccaca
     2221 gcctctccct gctgggaacc gtggcgtacg ccatcggggc ggcggtgggt ttcacgatgg
     2281 ttctggtggt cttcgcggcc atgcgggaac gcctcgccca cgccgaagtg ccggcggcat
     2341 ttcgcggtgc gcccattacc ctgatcagcg cgggaattct ctctcttgcg ttcctcgggt
     2401 tcgcggggct cggcaactga tgggcagcgc aggtccccca tgctgaccgc tttggtactg
     2461 actgccgtgc tgaccgccgt ggtggccgcc ctgggggccg cgctcgtctg ggcggaccgg
     2521 cgctacccgc cggatacgcg gacgctggtg gagtcgatcg acggtctgct cccccagacc
     2581 cagtgcgcgc agtgcggata ccccggctgc cgaccctacg ccgaggccgt cgccgcgggc
     2641 gcggccatca acctgtgccc tccaggcggt cccgagacac accgggccct cgcccggttg
     2701 ctcggccgca gcgaagaaga cccaccgcag gaggcttcca agctcaaagc ccgcatccga
     2761 gaggacggct gcatcggctg ttacctgtgc gtggaggcat gccccgtgga cgccatcgtg
     2821 ggcgcgccgc gctatcttca cacggtcctc gaggcggcgt gcacgggctg cgagctttgc
     2881 gtcccggcgt gcccggtgga ctgcatcgac atgacgcccg cccgggaggc ttcccccatc
     2941 agccatcagc cgcgccggcg atggccggac cggcagagct caccatgcat caggtgcgga
     3001 gcctgccagc ccgcctgccc ggtggggctg agcccccagg agctgctctg gtacgccggg
     3061 ggctcctcca gcgggaggcc taccggtcag gcggccgccg cgacccgcgg cctggatcgc
     3121 tgcatcgagt gcgggctttg caacccggtc tgtcccagca acatcgacct cgtgggcgtc
     3181 tttcgccaag cgcgacgagc gcaggccgca aggcacgaag ccgaacggga cgccgccgat
     3241 gccaaggcgc ggcatgcaag acgcaccgaa cgcctcgagc agacgaccgc caagcgcgct
     3301 gaagatcgcg agcagcgcat tgcccgacgg agcggcaggc aatggcgggc ctgagctccc
     3361 gcgggatcat gatggccgtc tgcctggcgc ttttgccggg cacagcgacg atgatctatt
     3421 tctacggggt cggcgtggtg cacaacctgc tgctcgccgt gcccgtcggg ctggccgtcg
     3481 aagccatctg cctgcagttg cagcagcgtt ccgtcggccg gacgctggcc gatggcagca
     3541 cggtactcac gtgcgtgctc atcgcgctcg ccctgccgcc cggcgcgccc tttgaaattc
     3601 tctccatcgc cgtcgttgcg gccgtagccc tggcaaaaca cctttacggc gggctgggga
     3661 gcaacctgtt caaccccgcg ctcgtggggt acgccgtcgt cctggtctcg tttcccgtgg
     3721 cgatggcgag ctggcccatc ccgatggacg gcgaaaccgg cgccacggcg ctggtgtcga
     3781 tgaagtaccg ggaggggctc accgtcgcgg aagcgtggca gggcgcacgc ggcttcggcg
     3841 ccatcggcgg ctacggctgg gagtggatca acctgggctt cctcctaggc ggggctgtgc
     3901 tcgtgtggcg gcggctggcg gcatggcgcg tgccggcgac cattctggcc accatcgccg
     3961 tgctggcggc cgcgttctgg gacggcggca gctcgaccag cagcgggtcc ccgctcttcc
     4021 actggttcac cggctccacg atgctgaccg tcttcttctt cgcaacggac cccgttacgc
     4081 acccggtttc gccgcgcggt cagatcgtct tcggcatcgt ggtgggcacc gcgaccttct
     4141 tgattcgggc cttcggtaac tatccggacg gcgcggcatt cgccatcctc ctcggcaacg
     4201 ccctcacgcc ctatctcgac aagcgtctgc aggaggcgtc ggcaaatgca taagctcgtg
     4261 ctcatcgcgg ccgcctgcgc gatgctgctc gccctcaccg atgcttggac ggcggatcgc
     4321 atcgcccgga acgcccgcag ccacgagctg agccaaatca ccgacctggc gggagcagcg
     4381 ccgcctgccg atacgacctg gaatgaggac gtctgggatt tctgcagcgg cacgaaactc
     4441 gcccgcagct cggcggcggg ttacggcggc ccgatccggt tggtcgtcgg cgtgtcgcaa
     4501 gggcgcatcc tgggcatcag agtcaccgcg caccaggaaa cgcccggcct tgccgacttc
     4561 ctgcgcgagc ccaagcgcgg ttggcttgcc gcattgaatg gccggaccgg accggaaacc
     4621 gcggccgtcg atgcggtagc gggggccacg atcaccagcg aagcggtcgt ccgcgcggtt
     4681 cgcgacgcca tggaccgggc gcaggacgcc agcgccgagg gatgccgcgc atgagcgtcg
     4741 ccaagccgtt ccgcaacgcc gccatcgcca gcaacccggc ctgggcgcag ctgctgggcc
     4801 tctgccccct gctcgctgtc agcaccagcc tggtcaacgc cctggcgctg ggcgcggcaa
     4861 gcgcgttcgt gcttctcggc agcaacatca ccatcgccgc catccgccga tggataccgg
     4921 agctcgcccg tcttccgagt ttcgtgctcg tcatcgccac gttcacgacg tgcgcgatgc
     4981 tcctgatgca ggcctacgcc ttcgagctgt atctgcgaat cgcgctgttc gtccagataa
     5041 tcgtcaccaa ctgcatgatc ctcggccggg tcgaggcgtt cgcgagcagg cagccggtgg
     5101 ggcgcgcttt cttggacgcg gcgggcacgg cttgcgggtt cgcgctggcc ctgctcgcca
     5161 tgggcgccgc ccgagagctc atcggccacg gctccctgat ggcgggaatg gagcagctga
     5221 ttcccggctc ggagtcatgg cggttgaacc tgtcggagag cggacgcggt ctgctcctgg
     5281 cgagcctgcc gcccggcgct ttcatcatcg ccggcctgct cctcggtgcg ggcaacgcga
     5341 ttctgcgccg accgaaccca gccccatccg gctccgcagg agaaaactca gcgtgaacaa
     5401 cgacaaacgc cgcgccatct tcgagcgcct acgggcggcc aacccggcgc cgacgacaga
     5461 gctcgactac cgcaacccct tcgagctgct gatcgccgtc atcctctccg cccaggccac
     5521 cgacgtcagc gtcaacaaag ccacgggccc gctgttcgag gcggccggaa cgccgaccga
     5581 gatgaaggcg ctcgggctgc gaaagctcaa gagctacatc aagaccatcg gcttgttcaa
     5641 taccaaggcg gccaacatct tgaagacctg cgagatgctg ctcacgcagc acggcggcga
     5701 ggtcccggag gatcgccaag ccctggaagc gctccccggg gtgggacgca aaaccgccaa
     5761 cgtggttctc aacacggcgt tcgggcaccc caccatcgcc gtggacaccc acatcttcag
     5821 ggtctgcaac cgcacccgga tcgcgccggg caagaacgtc aatcaggtgg agcagaagct
     5881 gctcaaggtc gtgccggacg agttcaagcg cgacgcgcac cactggctga tactgcacgg
     5941 ccgctatacc tgcatcgcgc gcaagccgcg ctgcggatca tgcgtgatcg aggatctctg
     6001 cgagtttcgt caaaagaccg atctcgaggg ctgaggtatc cgcggcgcgg ccgcgcaggc
     6061 gggcactcag gacgactttc tgaaccagcc gatgccgcga gttttcaccg tcgacgaagc
     6121 cgcgccctgc agcgcctttc cgggcgcggc gcccgctcgc ccgagcagac cgtcctgctt
     6181 ggcgcgcagc tcgcgtatct tcttctcgat gatcaggtcc tctttctgcc gctgccggac
     6241 ggctgccgcg atgtcgacac cgcagcgcgt gcagtgagcg gcttccagct gcatgtgccc
     6301 gcagcgcggg cactccgtgt gagcagcggt gatgtcgcgc agggtcgcgt cggatttacc
     6361 ggacctgtcc acccggaagt ccgccacgtt gttgcgggtc ttcggcgtga ggtctttgac
     6421 ccggcatatg gcgcccaggc tggctagacg ctcctgcagc tccatggcct gccgatggct
     6481 gagttgactc ttgatgacca ccgtccggcc gctgaaaagc tgcgcggctt tgcggtcgtc
     6541 gatgtcgagt tcgcaggcca gctttgcccg cacgatgtcc ctattcgcct gcggggaaac
     6601 ctcaccgctg aacagcactt gatagttgtt catcgattac tccgaaagct ttcgtcctct
     6661 ccttgcgccc aagcgcaggc ggagggcgtt aaggcggatg aaacccgccg cgtcggcctg
     6721 atggtacgcc cccccgtcgt cctcgaaagt gacgacgttc gcatcgtaca ggctctcctc
     6781 ctgcgacatc cgtcccacga cggataccgc acccttgtac aacttgagcc taacccgacc
     6841 cgagacgggt ttctgtgatt cgtctatcag cgcttggagg acttttctct caggagacca
     6901 ccagtagccg ttgtagatga gcgtcgcgta ccggggcatg agctcgtcct tgagatgggt
     6961 gacttcccgg tccagcgtga gggactcgat ggcgcggtgg gccttgagca gcagagttcc
     7021 ccccggtgtc tcgtagcagc ctcgggactt catcccgacg aagcggttct ccacgatgtc
     7081 gctgcgaccg ataccgtggg cgccccccag ggaattgagc ctggcgagca gatcggccgg
     7141 tccgagcttc tcgccgtcga cggccacggg atcgccctgc aggaaatcga tctcgacgag
     7201 ctgcgcctcg tccggcgcct cctcgggcgc caccgtccag cgccacatgt cggcatccgc
     7261 ctcctgccag ggatcttcta ggttgtcgcc ttcgtaggag atgtggagca ggttggcatc
     7321 catggagtaa ggagacttgg cgccgcgctg ataatccacg gggatctggt gctgctcgca
     7381 gaacgccatc aacgcctccc gggaattgag atcccactcg cgccacggcg cgatcacccg
     7441 aatgtccgga ttcagcgcgt atgctccgag ctcgaagcgg acctggtcgt tgcccttgcc
     7501 ggttgctccg tgagcgacgg cctccgcgcc ggtttcagcg gcgatctcga cgagacgctt
     7561 ggcaatcaga ggccgcgcaa tgctcgtccc gagcaggtac tcgccttcgt agagagtatt
     7621 tgcgcggaac atcgggtaga cgtagtcgcg cacgaactcg tcgcgcagat cctcgatgaa
     7681 gatctcctgc acgcccatgg cctgggcctt cgccctggca ggctccacct cctcaccctg
     7741 gccgatgtcc gcggtgaacg tcaccacctc ggcaccatag gtttcctgca accagcggca
     7801 gatcacggag gtatccaggc cacctgagta tgccagcaca atcttcatcg tcacgtttcc
     7861 cccgagtgcg gtcgccgaga aaaaagggtc gcaatcttaa gtaacttgag cagggcccgc
     7921 cagccgccgt cccgcgggaa acccttccgg ccgggtgcct tcccgcttcc ggcgcgcaaa
     7981 gtcgcgtcaa agccgtgtta atgtgcccgc atggagatgg cggatcgttt tcggggtttt
     8041 ctgccggtgg tggtcgatct ggagacggga ggcttcgatc ccggctccca cgccattctg
     8101 gagatcgcag ccgtgcagct gcgtttcgat cgcgggcgga tcgcgccgga ccggaagtgg
     8161 catcaggccg tggagccctt cccgggcagc cgggtggagg ccgcttcgtt gaag
//
LOCUS       DBNHLM010000134         7419 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1155536, whole genome shotgun sequence.
ACCESSION   DBNHLM010000134 DBNHLM010000000
VERSION     DBNHLM010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7419)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7419)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7419
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1155536"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..1112
                     /locus_tag="AC16QF_06265"
     CDS             291..1112
                     /locus_tag="AC16QF_06265"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448193.1"
                     /translation="MDLGLEGKKVIITGGSRGLGRAIGETFLREGAVVSFCSRTGRIE
                     PEDEDPRLGGEVRTDGVEQAAEKLAALGEVYGSVVDAADREQLARWVNEAAETMGGID
                     VVVSNTSALGGQPRSPEGWQRQFDVDIMSAVTLWDNAYPFLKASGAGAFVQVSTITAV
                     DYHAYGQACQSYGAFKGALVNYVCQLAYEYMSEGIRANCVSPGPIFVTGGSWDKIQRA
                     LPEYYEENVARQPAGRLGRPEEVADVVAFLASPRASWVTGENIVVDGAFTKYIKY"
     gene            1122..2570
                     /locus_tag="AC16QF_06270"
     CDS             1122..2570
                     /locus_tag="AC16QF_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448194.1"
                     /translation="MQSAQPDGQVLVADARAASEVLHEVLADTAAAFPGALGPMGFPA
                     DPKAFRSTYPEVMPRFEAARLASGDRLAMARHAVGALQRRLVWQDAGGSRPLADALAE
                     PAAPLELLQTDFPGEPGWRPSVVYRGERWEADRLADFGAELLGRGVVTAQASQALAWL
                     ADNVLDAGVLRLTGRKIAVLGGGAEMAPTRLWLEAGADVLWLDVAPPPPSWQDASAMS
                     GRLYWPAGNADLLAQPREILATLIEFAAGRPLDLGLYAYAPGRARELRLTGVMNELVN
                     AMPPELVGSITVLVSPTTPTGLAEPDLAAMRARREARPGWEAALDNLGLLGRGGGCAT
                     MGEVAATRTVVGIQGASYQAAQYLGKVLVAECWAEEGPLGSAHPAPFRVSANTAAITR
                     TRSLGHPVFAAAFGGAAAFGVETLTPRQSRRINGLLALCDWLRPDRPVPGTVRVHGGI
                     HTLPYPLESALRIAAAIGFARSPRLLRGLFGK"
     gene            complement(2626..3507)
                     /locus_tag="AC16QF_06275"
     CDS             complement(2626..3507)
                     /locus_tag="AC16QF_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6448195.1"
                     /translation="MQTWQVGDVKVTQIVELVFTGLDAFLPDATPEAVLPIEWLRPHF
                     ITPEGVLRFSIHALVIDTGARKIIVDTCVGNDKPRDVFPDWHMLQTGFLEDLASAGYP
                     RESIDTVLCTHLHLDHVGWNTMLEGGQWVPTFPNARYLLERSEFAYVAQEAEAEDVEP
                     WLRDMNRAVMADSIRPVVDAGLIDLVDADHRLCEEVRLMPTPGHTIGHVSVHIASRGE
                     SALITGDFIHHPCQLAHPEWSVTTDYDPSDSVSTRERVFAELADGPTLVIGTHWPEPT
                     AGKIASWGDTYQLKTDP"
     gene            3656..3928
                     /locus_tag="AC16QF_06280"
     CDS             3656..3928
                     /locus_tag="AC16QF_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448196.1"
                     /translation="MTRLRSVLLVLTLLVFSSQSIAHDHLSDDLEMDPSCLVCSYSDS
                     VPLTGPVSLTPLWADPAGAKAWPPALGPTPRDCPKDYLSRGPPFSR"
     gene            4004..6058
                     /locus_tag="AC16QF_06285"
     CDS             4004..6058
                     /locus_tag="AC16QF_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6448197.1"
                     /translation="MQRLFFGLCSCFAAASGQAQPSSSEPIEEVVVIAHPLSGEGLAQ
                     AATVLEGRELDRKRASTVGETLGREAGIHSAQFGKAVGRPVIHGLGGARVRVLEDRID
                     AMDVSVTSADHAVTVEPFVADRVEVLKGSSALLYGSGAIGGVVDVHTGRIPHEVPDRP
                     LTGGIETRFDSNNDGNATSGKLDGAVGDFAWHVDGTWKDGDDYEIPGFAESEGLRALK
                     SAATSGSLGEEDEAHDRLPGSAFDSESYAVGLSRVGEWGFLGGSVSRLEADYGLPGGH
                     GHAEDGDAGGGLGDDHGDTPTLALKQTRTDFELGILDPFGSFTSANLRLGINDYEHAE
                     IEPDGAVATEFANEAWELRAELVYELEAWRGALGLQHSDRDFSAIGEEAYVPPVQTTD
                     SGLFWVAERDFGGFDLEAGARIGRLEHEPLGAPSERFTTYGLSLGAVVPLGAGWRLGL
                     LGDLSSRAPVAEELYSDGPHLATRAFEIGDPALDSEKAFNLSATLEFEGERWSLAGTV
                     YYTGFSDFIYQQATGGVEDGLPVYVYRQEDARFVGVDASVEWRAAAWEGGDLRLRGLF
                     DLVDAKLDVSGNDNLPRIPPMRYGVGAQMSFGPVTASLDYLRVEDQDDAAPGELPTQG
                     YDDLQAYLGVELPFAADHRLTMFVSGRNLTDDEQRLHTSFIKDFAPAPGRTIEAGLRY
                     LF"
     gene            complement(6067..7413)
                     /gene="gdhA"
                     /locus_tag="AC16QF_06290"
     CDS             complement(6067..7413)
                     /gene="gdhA"
                     /locus_tag="AC16QF_06290"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014755289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HIR6448198.1"
                     /translation="MTITAPDLDQFMRGLRRRNPGESEFHQAVQEVAIDILPYIADKP
                     AYTAKRIFERMTEPDRIVSFRVVWEDDAGNVRVNRGYRVQCNNAIGPYKGGIRFDPSV
                     NQSILKFLAFEQTFKNSLTGLPLGGAKGGADFDVKGKSDSEVMRFCQAFMTELYRHIG
                     PFTDVPAGDIGVGSREVGYLFGQYKRLANRFEGVLTGKALEFGGSHIRTEATGYGVVY
                     MMENMLAHRGESLEGRTCAVSGSGNVAIYTVEKLNELGARVVTMSDSSGFIHDPDGID
                     ADKLAWILDLKTVRRGRVSEYADEFGVEFQEGKRPWAVPCELAFPCATQNEIGKEDAE
                     TLVANGCCAVVEGANMPTVPEALEVFTSARTLLAPSKAANAGGVGVSGLEMSQNSVRL
                     GWTREEVDTRLRQIIKEIHGHCVEHGDEGDYVNYLKGANIAGFRKVAAAMLAYGVM"
BASE COUNT         1153 a         2357 c         2543 g         1366 t
ORIGIN      
        1 ctcaccgtcg agcaggttcg cattgctgta cagaactcgt ggcatgttgt ccctcccgtg
       61 gcggagatgg tcggtgcaac ggccgatggg tgatgagcct cggcacgact tgcccccaac
      121 acccaacagt atcactggcc cggaccagac gcatccggag atcgcctgtg agttcggcgt
      181 gttcgccgga tgactttggg gaacactcca accggtgaga gtcgggtgca cggcagcggc
      241 gctggccggg ttcgctatac ttgcctgatc gggttttggg ggtgtgagtc atggatcttg
      301 ggctcgaagg aaagaaagtc atcatcaccg gcggctcgcg ggggctgggt cgcgccattg
      361 gggagacgtt cctgcgcgaa ggagccgtcg tgtcgttctg ttcccgcacc gggcgtatcg
      421 aacccgagga tgaagatccg cgccttggcg gggaggtccg gacggacggc gtcgagcagg
      481 cggcagagaa attggctgcc cttggcgagg tgtacgggtc cgtcgtggac gccgccgacc
      541 gcgagcagct cgcgcgctgg gtgaacgaag cggccgagac catgggcggc atcgacgtgg
      601 tggtttcgaa caccagcgcg ctcggggggc aaccgcgttc gccggaagga tggcagcggc
      661 agttcgacgt ggacatcatg tccgcggtaa cgctgtggga caacgcctac ccgttcctca
      721 aggcttcagg agcgggcgcg ttcgtgcagg tctcgacgat taccgccgtg gactaccacg
      781 cttacgggca ggcgtgtcag agctacggcg ctttcaaggg agcgctggtc aattacgtgt
      841 gccagcttgc ctacgagtac atgagcgaag gcatccgcgc caactgcgta tccccggggc
      901 cgatcttcgt caccggcggt tcctgggaca agattcagcg ggccctgccc gagtattacg
      961 aggagaacgt ggcgcgtcag cccgcgggtc ggctgggcag gccggaggag gtggccgacg
     1021 tggtggcctt cctggccagc cccagggcgt cctgggtcac gggggagaac atcgtcgtgg
     1081 acggtgcctt caccaagtac atcaagtatt agccgttcgc agtgcagtcc gcacaacccg
     1141 atgggcaggt cctggtcgca gacgcgcgcg ctgcgtccga ggtgctccac gaggtgcttg
     1201 ccgacaccgc cgcggccttt cccggcgccc tgggtccgat ggggtttccc gcggatccca
     1261 aggcattccg ttccacgtat cccgaggtga tgccccgttt cgaggcggcc cgtctggcca
     1321 gcggcgatcg ccttgccatg gcacgccacg cggtcggcgc cctgcaacgg cggctggtct
     1381 ggcaggatgc cggcggtagc cggcccctgg ccgacgcgct ggcggagccg gctgcgccgc
     1441 tcgagctcct gcagaccgac tttccggggg agcccggctg gcggccgagc gtggtgtatc
     1501 ggggagagcg ttgggaagcc gatcggctcg ccgatttcgg cgcggagctg cttggccgcg
     1561 gcgtcgtgac cgcgcaggcg tcccaagccc tcgcctggct ggctgacaac gtgctggatg
     1621 ccggagtgct gaggctgaca ggacgcaaga tcgcggttct cggcggcggc gctgagatgg
     1681 cccccacgcg gctttggctc gaggcagggg ccgacgtgct ctggctcgac gtggcgcccc
     1741 cgccgccgag ctggcaggac gcgtcggcca tgtccggtcg actgtactgg ccggccggaa
     1801 acgccgatct gctcgctcaa cctcgtgaaa tcctcgctac gctgatcgag tttgccgccg
     1861 gcaggcccct cgacctcggc ctgtacgcct atgccccggg gcgggcgcgg gaactgcgac
     1921 tcacgggggt catgaacgag ctggtgaacg cgatgccgcc ggagctggtg ggcagcatca
     1981 ccgttctggt ctcgccgacc acgccgaccg gcctcgccga gccagaccta gccgccatgc
     2041 gcgcgcgcag ggaagcccgc cccggctggg aagccgcgct cgacaatctc ggcctgctgg
     2101 gaagaggggg aggctgcgcc accatgggag aagttgcggc aacccgcacg gtggtgggca
     2161 ttcagggagc cagctatcag gcggcccagt acctgggcaa ggtgctcgtg gcggaatgct
     2221 gggccgagga aggcccgctg ggcagcgctc accccgcacc gtttcgggtg tccgccaaca
     2281 ccgcggcgat cacgcgcacg cgctccctgg gtcacccggt ctttgccgcg gcgtttggcg
     2341 gggcggcggc attcggcgtg gagacgctga ctccccgcca gtcgcggcgc atcaacgggc
     2401 ttctggccct ttgcgactgg ctgcgcccgg accggcccgt gcccggcacc gttcgggtgc
     2461 acggcggcat ccacacgctg ccgtatccgt tggagtcggc gctgcgcatc gcggccgcca
     2521 tcggctttgc gcgctcgcct cggctgctgc gaggcctgtt cgggaagtga tcggctcggg
     2581 gctcgatgtt ccggtctcgg gccgttgggg tcggggagcg gcagctcagg gatccgtctt
     2641 gagctggtag gtgtcgcccc agctggcgat tttcccggcg gtgggttccg gccagtgggt
     2701 gccgatcacc agggtagggc cgtcggccag ctcggcaaag acccgctctc tcgtgctcac
     2761 ggaatctgaa gggtcgtagt cggtcgtcac ggaccattcc ggatgggcga gctgacaggg
     2821 gtggtggatg aaatcgccgg tgatcagggc ggactcgccg cgggaggcga tgtgcacgct
     2881 gacgtggccg atggtatggc cgggcgtcgg catcaaccgc acctcttcgc acaggcgatg
     2941 gtcggcatcc accagatcta tcaggccggc gtccaccacc gggcggatgg agtccgccat
     3001 cacggctcgg ttcatgtcgc gaagccaggg ctcgacgtct tccgcttctg cttcctgggc
     3061 cacgtaggcg aactcgctgc gttcgagcag gtagcgggcg ttggggaagg tcggcaccca
     3121 ctggccccct tcgagcatgg tgttccaacc cacgtgatcc aggtgaagat gcgtgcagag
     3181 caccgtatcg atcgactctc gcgggtagcc tgccgaggcc agatcttcca ggaagccggt
     3241 ctgcagcatg tgccagtcgg ggaacacgtc cctcggtttg tcgttgccga cacaggtgtc
     3301 gacgatgatc ttccttgctc cggtatcgat gaccagcgcg tgaatgctga atctcagaac
     3361 gccctcaggg gtaatgaaat gcggccgcag ccactcgatg ggcaggacgg cttccggcgt
     3421 cgcatcgggc aggaaagcgt ccaacccggt gaacaccagc tcaacgatct gggtgacctt
     3481 gacgtctccg acctgccacg tctgcactgg gcgtttcctt gatccgtggc catcgcgctt
     3541 cagacggaga gattagaaca tggctggttg gccggtgctc ggcttgcgcc gtgcctgcat
     3601 cgctgatagt ttccgccccg cactcccgtt gatgtcagga acggctttct tgccgatgac
     3661 gcgcctacga tcggttctgc tggtgctgac gctgctcgtc ttctcgagcc agagcatcgc
     3721 gcacgatcat ttgtccgatg atctcgagat ggatccctcg tgcctcgtct gcagctactc
     3781 agactcggtg cccctgacgg gtccggtctc cctgacaccg ctttgggcag acccggcggg
     3841 ggccaaggcg tggcctccgg ctttgggccc gacgccccgc gattgcccca aggactacct
     3901 gagccgcggc ccgccctttt cccgctgatt ccagcacgca ttcagatgag ttgatgccgc
     3961 agcctgcttt cgtcgggcga gcgccaatcg atgggaacag gacatgcaga gattgttttt
     4021 cgggctgtgt agttgcttcg ccgccgcgtc ggggcaggca cagccatcat catcggagcc
     4081 gatcgaagag gtcgtcgtga tcgctcaccc tctctccggt gagggcctcg ctcaggctgc
     4141 aaccgtgctg gaagggcgcg agctcgaccg caagcgcgct tccaccgtcg gcgagacgct
     4201 cggccgggag gcaggcattc actccgctca gtttggcaaa gccgtgggtc gtccggtcat
     4261 tcacgggctg ggcggcgcgc gggttcgcgt gttggaggac cgcatagacg ccatggacgt
     4321 gtcggtgacc agcgccgatc acgcggtcac ggtggaaccg ttcgttgctg accgcgtgga
     4381 ggttctgaag ggatccagcg cgctgctcta cggctctggc gccattggcg gcgtggtgga
     4441 cgttcacacg ggccgcattc cccacgaggt gccggaccgg ccgttgaccg gcgggatcga
     4501 aacccgcttc gacagcaaca acgacggcaa cgccaccagc ggcaagctcg acggcgccgt
     4561 gggtgatttc gcctggcacg tcgatggtac ctggaaggac ggcgacgact acgagatccc
     4621 gggctttgcg gagtccgaag gtctgcgtgc gctgaaatcg gcggcgacga gcgggtcgct
     4681 gggggaagag gacgaggccc atgacaggct gccgggcagc gcgttcgatt cggaatccta
     4741 tgcggttggt ctttcccggg tgggtgagtg gggcttcctc ggcgggtccg tgagccggct
     4801 cgaagccgat tacggcctgc ctggggggca cggccacgcc gaagatggcg atgcgggcgg
     4861 gggtctcggg gacgatcacg gggacacgcc caccctggcc ctgaagcaga cgcgtaccga
     4921 cttcgagctt ggcatcctgg atccgttcgg gtcgtttacc agcgccaatt tgcggctggg
     4981 aatcaacgac tacgagcacg cagagatcga accggacggc gcggtggcaa cggagttcgc
     5041 caacgaggcc tgggagcttc gggccgagct ggtatacgag ctcgaggcct ggcgcggggc
     5101 gctcgggctg cagcactcag accgtgattt ctccgccatc ggggaagagg cctacgtgcc
     5161 tccggtgcag acgaccgaca gcggcctgtt ctgggttgct gagcgggact ttggaggttt
     5221 cgacctggaa gcgggcgcgc gcatcggccg gctggagcat gagccgctgg gcgcgccgag
     5281 cgagcggttc actacttacg ggctctccct tggtgccgtg gtgcccctcg gggcgggatg
     5341 gcgcctcggt cttctgggtg acctctcttc ccgggcgccc gttgccgagg agctctattc
     5401 cgatgggcct caccttgcaa cccgcgcttt cgagatcggg gacccggcac tggactccga
     5461 gaaggctttc aacctgtcgg cgaccctgga attcgaaggc gagcgctggt cgttggccgg
     5521 aaccgtttac tacaccggtt tttcggactt catctatcag caggcgacgg gaggcgtgga
     5581 ggacggcctg ccggtctacg tctaccggca ggaggatgcg cgcttcgtgg gcgtggacgc
     5641 ctcggttgag tggcgggccg ctgcctggga aggaggcgat ctccgccttc gggggctgtt
     5701 cgacctcgtc gatgccaagc tcgacgtcag cggcaacgac aacctgcccc gcataccccc
     5761 gatgcgttac ggcgtcggcg cccagatgtc gttcgggcct gtcacggcct cgctggatta
     5821 tctcagggtc gaggatcagg acgacgcggc gccgggggag ctgccaaccc aggggtacga
     5881 cgaccttcag gcatacctag gtgtggagtt gcctttcgcg gccgatcatc gcctgacgat
     5941 gttcgtctcc ggcagaaacc tgaccgatga cgagcagcgt ctgcacacct cgttcatcaa
     6001 agacttcgcc ccggcgcccg gccgtaccat cgaagccggg ctgcgttacc tgttctgacg
     6061 ggcttctcac atcaccccat aagccagcat ggccgccgcc accttccgga agccggcaat
     6121 gttcgcgccc ttgaggtagt tcacgtagtc gccttcgtcg ccatgctcca cgcagtgacc
     6181 gtgaatttct ttgatgatct ggcgcaggcg cgtgtcgact tcctcgcgcg tccagcccag
     6241 acgcacgctg ttctgactca tctccagccc ggacacgccg actccgcccg cgttcgccgc
     6301 cttggagggc gcgagcagag ttcgggcgct ggtgaagacc tccagcgctt cgggcacggt
     6361 aggcatgttg gcgccctcga ccaccgcgca gcagccgttg gcaacgaggg tctcggcatc
     6421 ctctttgccg atctcgttct gggtcgcgca gggaaatgcg agctcgcagg gcaccgccca
     6481 gggccgtttg ccttcttgga actcgacgcc gaactcgtcc gcgtactcgc tcacgcgacc
     6541 gcggcggacg gttttgagat ccagaatcca ggcgagcttg tccgcatcga tcccgtccgg
     6601 gtcgtgaatg aatcctgatg agtcggacat ggtgaccacc ctggcgccga gctcgtttag
     6661 cttctccacg gtgtagatgg ccacgttgcc ggagccggat acggcacacg ttctgccctc
     6721 gaggctctcc cctcgatggg cgagcatgtt ctccatcatg tagaccacgc cgtagccggt
     6781 ggcttcggtg cggatgtggc tcccaccgaa ctcgagcgcc ttgccggtca gcacgccctc
     6841 gaagcggttg gccagccgct tgtactgacc gaacaggtag ccgacctccc gggacccgac
     6901 cccgatgtct ccggcaggca cgtcggtaaa aggcccgatg tgtcggtaca gctccgtcat
     6961 gaacgcctga cagaaccgca tgacttccga gtcggacttt ccctttacgt cgaagtccgc
     7021 gccgcccttg gcacccccca ggggcagacc ggtgaggctg ttcttgaagg tttgctcgaa
     7081 ggcgagaaac ttgaggatgc tctggttgac cgacgggtcg aagcggatgc cgcccttgta
     7141 aggtccaatg gcgttgttgc actgaacccg atagccgcgg ttcaccctca cgttcccggc
     7201 gtcgtcctcc cagacgactc tgaaggagac gatgcgatcc ggttccgtca tgcgctcgaa
     7261 gatccgcttt gctgtgtagg cgggcttgtc agcaatatac ggcaggatgt ctatggccac
     7321 ttcctgcact gcctggtgaa actcggactc gcccgggttg cggcggcgca ggcccctcat
     7381 gaactggtcc agatcgggag cggtgattgt catggcgct
//
LOCUS       DBNHLM010000135        15368 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_318307, whole genome shotgun sequence.
ACCESSION   DBNHLM010000135 DBNHLM010000000
VERSION     DBNHLM010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 15368)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15368)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15368
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_318307"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /gene="hisG"
                     /locus_tag="AC16QF_06295"
     CDS             complement(<1..580)
                     /gene="hisG"
                     /locus_tag="AC16QF_06295"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007352404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIR6448199.1"
                     /translation="MGLTIALNRGRILEESLPMLAAAGIVPEEDPAASRKLIFATRQG
                     GHRLVVMRGADVPTYVEYGAADVGIVGKDTLLEHGGSADYYERLDLGIAKCRIMVAAP
                     AGADLGSGKLRVATKFPRVARRYFEGQGRQVSIIKLNGAMEIAPLMNLADCIVDIVDT
                     GNTLRANGLEALETIAHVSSRMIVNRAAMKTRF"
     gene            complement(596..1861)
                     /gene="murA"
                     /locus_tag="AC16QF_06300"
     CDS             complement(596..1861)
                     /gene="murA"
                     /locus_tag="AC16QF_06300"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR6448200.1"
                     /translation="MDKLRITGGRTLSGSLRVSGAKNAALPILAGTLLADEPVTLSNV
                     PHLQDVTTMIELLGCMGARVLVDEKMRVEVSADSLAQLRAPYELVKTMRASFVVLGPL
                     LARFGEAEVSLPGGCAIGARPVDQHVKGLEALGAEISVNEGYVRGKVGGRLRGADFAF
                     DMVTVGATENLMMAAALAEGVTRLRNAACEPEVADLGDFLNAMGAKVSGHGSPEIRIE
                     GVPRLHGCEYRVMPDRVEAGTYLIAVAATGGRIRLTDVRPDTLTAVLDKLVESGAEVE
                     VGDDWIELDMHGRRPRAVDITTAPYPGFPTDMQAQYLAMNAIAEGTSEVSETIFENRF
                     MHVQELSRLGADIELHGPSSVSVRGVPSLKAAPVMATDLRASFSLVVAALVAEGTTLI
                     DRIYHIDRGYETIEEKLKLLGAEVQRVSG"
     gene            complement(1854..2105)
                     /locus_tag="AC16QF_06305"
     CDS             complement(1854..2105)
                     /locus_tag="AC16QF_06305"
                     /inference="COORDINATES: protein motif:HMM:NF013851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HIR6448201.1"
                     /translation="MQEEIAASITERLSGEDDALRVDVVVEGNRAMITVVSRMFEGMT
                     RVRKQQTVYGCIDAFIQDGRLHAVSIRSLTPEEAAGSSG"
     gene            complement(2135..2425)
                     /locus_tag="AC16QF_06310"
     CDS             complement(2135..2425)
                     /locus_tag="AC16QF_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024857.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR6448202.1"
                     /translation="MSAFSLPPRVGVDNFAAVRAAGEQFLAERRDEKAVVDLSGLESC
                     NSIAVALLMTWFRFAHARGQEVVYAGVPQDLLSIIEISGLMEVLPLSRDAAG"
     gene            complement(2422..3177)
                     /locus_tag="AC16QF_06315"
     CDS             complement(2422..3177)
                     /locus_tag="AC16QF_06315"
                     /inference="COORDINATES: protein motif:HMM:NF017319.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaC/ttg2D family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR6448203.1"
                     /translation="MTMRLMNHSGLESGFVLAIAALLLAGLAPARAMEPSDVATRTAT
                     EVVERTSDEMLALIDASRPYVEDDPERFYGEVESLLAPVVDFKRFARSVMAVHYKSAT
                     PEQRDRFADTFKWGLVRTYALALTEFRDGEVVIVPSERPPQSPRRESVKMEIRMNTGD
                     VYPVVYSMAQGKDGAWRMGNIIVNGVNIGLTYRSQFASAVQDQRYGGDLDKVIDAWTL
                     LLANETPGAASADSGGSDEAPSSDGAGGAGDDR"
     gene            complement(3177..3635)
                     /gene="mlaD"
                     /locus_tag="AC16QF_06320"
     CDS             complement(3177..3635)
                     /gene="mlaD"
                     /locus_tag="AC16QF_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HIR6448204.1"
                     /translation="MHIRTLEISVGAFMLAGILALVFLAVKVSGISLDADRGSYQLVA
                     RFDDVAGLRERAKVSIAGVQIGRVALIEVDTIYGQAVVTLEIDGSPGGLSADTGAQIL
                     TEGILGARYVNLLPGADDETLADGDLIYDTQGALVLENLVGDLITRLGAG"
     gene            complement(3635..4417)
                     /gene="mlaE"
                     /locus_tag="AC16QF_06325"
     CDS             complement(3635..4417)
                     /gene="mlaE"
                     /locus_tag="AC16QF_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HIR6448205.1"
                     /translation="MIQFLRSFGGRSLDRMASLGRATIMWFTALAGLPGPGDLRLLVR
                     QIYHVGVLSLIIIVLSAFSIGAVIALQFHTQLARFGAEDSVGLGLALVLLRELGPVVT
                     GLLFAGRAGSAITAEIGLMKTTEQLSSMEMMGVDPLRRIVAPRLWAGVISMPLLTAIF
                     NVVAIYGGLVVAVEWLGGDPGGFWSGMQDAVELWHDVGKGMLKSVVFAVVVTWIAVFQ
                     GYDTEPTAEGIGLATTRTVVASSVMVLAIDFLLTLSMYGEFN"
     gene            complement(4414..5229)
                     /locus_tag="AC16QF_06330"
     CDS             complement(4414..5229)
                     /locus_tag="AC16QF_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6448206.1"
                     /translation="MNGADDNLIEIRNLSFVRGDRVIFDDLSLSVPRGKITAIMGPSG
                     TGKTTLLRIIGGQVRPDSGVVLIDGQDVCGMSRRELFELRKDMGMLFQSSALFTDLNV
                     FENVAFPLRVHTDLPEDTVRDLVLMKLNAVGLRGARDLMPSQLSGGMSRRVALARAVA
                     LDPNLLMYDEPFTGQDPIAMGVLLSLIESLNDSLKTTSLVVSHDIHETISIADQIYLI
                     ADGTIMGSGTPDTLAVDDSPRVHQFMHGSPDGPVPFHYPADEFLYDVLGDVNA"
     gene            5358..6329
                     /locus_tag="AC16QF_06335"
     CDS             5358..6329
                     /locus_tag="AC16QF_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HIR6448207.1"
                     /translation="MLLSIAALIVGFMALLWSADRFLAGAASTAGHLGVSKMLIGLTV
                     VSLGTSAPEILVALTAAVEGNPLLAVGNAIGSNIANIGLVLGITAIIVPLPFSQTVLR
                     AELPWLLGATFLALALLFDLEMSALDSVLLLAGLGVILWRLVRSQKTAGSDLAESITE
                     ELEDLPQMSRGQAAVWLLIGLLILLASAEVLVWAASDIADRLGVNDIIIGLTIVAIGT
                     SLPELAATVGSALKGHTDIAIGNVVGSNILNILAVLAVPSLVDPILIENVWLWRDGGM
                     MLALTLLLAVFAYAVGSQPVITRFEGTVMLCAWVGYNLLLVSQAQLG"
     gene            6329..6844
                     /locus_tag="AC16QF_06340"
     CDS             6329..6844
                     /locus_tag="AC16QF_06340"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HIR6448208.1"
                     /translation="MAFPAPVLERAADIELVAFDVDGVLTDGKLYYSDDGRELKAFHV
                     QDGAALKLLLSHGIVVAIITGRSSSIVTRRADELGIAHVYQGVADKAAVLAALAETVG
                     TTLERAAYVGDDLPDLGVFSRAGMAISVPNAHPAAAAAADYVTLAPGGAGAAREIAQL
                     FLEARGEWPYG"
     gene            6856..7485
                     /gene="lptC"
                     /locus_tag="AC16QF_06345"
     CDS             6856..7485
                     /gene="lptC"
                     /locus_tag="AC16QF_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIR6448209.1"
                     /translation="MRKRSKWERWSTWTAILGVAVAGVVLLSEPLEPETEAETPPAGI
                     AEEPDLYVEDAVITQYLPSGTVHYRLVSKLLRQFEADELVRLTKPNLVVNSETRSPWQ
                     VQSEHGYVRRMGGPERGGEGEEVVFLREDVQLEQREPDGRHIRLTTETLYVYPEREYA
                     ETDRDVMIDTHAGRTTATGLKGDLQRGLLQLSSSDRRRVHTILLPDQFK"
     gene            7499..7981
                     /gene="lptA"
                     /locus_tag="AC16QF_06350"
     CDS             7499..7981
                     /gene="lptA"
                     /locus_tag="AC16QF_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HIR6448210.1"
                     /translation="MTLGAVLLAPGALALPEDADQPIHIRANSAEMDQEQGLVIYRGD
                     VQVDQGTLRVMADEMTVEYEDQEVIRITAKGEPARYQQQIEVDEEVVRARSSTIIYHT
                     RDERIDLVGNAFLTQEGNEITGDLIEYDIVAGKVDAKAESETPVRMILQPARKKPDGG
                     "
     gene            7990..8715
                     /gene="lptB"
                     /locus_tag="AC16QF_06355"
     CDS             7990..8715
                     /gene="lptB"
                     /locus_tag="AC16QF_06355"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003313594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIR6448211.1"
                     /translation="MPTLEVRSLRKQYRNKVAVEDVSLHVSTGEIIGLLGPNGAGKTT
                     TFYMIVGLLRADGGDIFLDEQRITRTPMHVRARAGIGYLPQEASVFRKLSTEDNVRAI
                     LELRRDLNRTQRQAMLDQLIEEFHLGGVRESLGERLSGGERRRLEIARALAMEPSFML
                     LDEPFAGVDPISVGDIKKEIRDLAARGIGVLITDHNVRDTLDICDRAYIVHEGHIIAE
                     GDSGAILADEKVKNVYLGESFSL"
     gene            8876..10351
                     /locus_tag="AC16QF_06360"
     CDS             8876..10351
                     /locus_tag="AC16QF_06360"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR6448212.1"
                     /translation="MKQTLSLKLGQQLTMTPQLQQAIRLLQLSCLDLRQEIQDALEGN
                     PMLELDEEDGGDEPTGSPAEADRAESDGDDDLDGVDATLEGDVIDDLPVDTSWDEIYQ
                     PAPAPAPAAGDSESWDFETRNAPEETLQDHLLWQLNLTPMSDSDRVIAMAIIDTISPD
                     GMLTTPVEELVTSLEGVVDEEAEIAHAEVMAVLKQIQHFDPIGVGARDLGECLRIQLE
                     QLPAGTPWLAESLTLVNEHLPLLASRDFAALKRRTKLGEEELGEVVRLIQTLNPRPGT
                     TVAQEAAEYVVPDVMVSRKEGRWLVELNPDATPGLRINAGYAGLVKRADNSADNTFLR
                     DHLQEARWFLKSLQSRNETLLKVATRIVEHQRGFLEYGEEAMKPLVLNEIAEAVEMHE
                     STISRVTSRKYMHTPRGIFELKYFFSSHVSTSSGGEVSSTAIRALIKKLTADENPRKP
                     LSDSRIATILAEQNINVARRTVAKYRESMAIPPSNERKRLV"
     gene            10408..10713
                     /gene="hpf"
                     /locus_tag="AC16QF_06365"
     CDS             10408..10713
                     /gene="hpf"
                     /locus_tag="AC16QF_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR6448213.1"
                     /translation="MQLSISGHHIDVTEALRNYVTEKMQKLERHFDHITNVHVVLSVE
                     KLRHRAEATIHASGTEVFADCDCDDLYAAIDGLTDKLDRQVLKHKEKLLDRQQRRGR"
     gene            10723..11190
                     /locus_tag="AC16QF_06370"
     CDS             10723..11190
                     /locus_tag="AC16QF_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011532414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR6448214.1"
                     /translation="MPDITTILRPDCTRASVEAPSRKRALEAAADLVAKHHPDLSARK
                     LFDELMARERLGSTGLGDGVAIPHCRIPCEQITGALLHLREAVDYESMDDEPVDLLFV
                     LAVPPEETTAHLDLLAALARIFGDPENRRRLRSSATDEALYQELVGLFSSQAA"
     gene            11187..12041
                     /gene="rapZ"
                     /locus_tag="AC16QF_06375"
     CDS             11187..12041
                     /gene="rapZ"
                     /locus_tag="AC16QF_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR6448215.1"
                     /translation="MKLIVISGRSGSGKSTALRALEDSGYNCIDNFPVDLLPHLIANA
                     TRAVEPQPDLAVCIDARNPDLEAFPQIIESLRSPSVDCEVIFLDAQSATLVKRFSETR
                     RRHPLTGTGKDLLQAIESEDAILQPIAGLADLSIDTTTMRGSALTELVRSRIAHKRSN
                     SLSLLFRSFGFKFGVPVDADFVFDIRCLPNPYWDESLRPLTGKDGAVASFLEAEAAVG
                     QMFSDIRSFLERWIAAFEKDNRAYMNIALGCTGGQHRSVYMAEKLAAHFSAQRPNVLV
                     RHRELAGS"
     gene            12049..12318
                     /locus_tag="AC16QF_06380"
     CDS             12049..12318
                     /locus_tag="AC16QF_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR6448216.1"
                     /translation="MREGRITIVNRLGLHARAAAKFVNLAKTFSSQVELTRDAERVDG
                     KSIMSVMLLAAPIGTELILHVVGPDEDEAFEALEQLVDDRFGEAE"
     gene            12370..13707
                     /gene="mgtE"
                     /locus_tag="AC16QF_06385"
     CDS             12370..13707
                     /gene="mgtE"
                     /locus_tag="AC16QF_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR6448217.1"
                     /translation="MDFQEQLSEVIRQYGSGSLDETRFLLESLRAPEIANLMESTPPR
                     VRRVIWGLLDEEDENQVIQHLREDVRADFLESMNTERLVAAGVDLEVDDFADILQQLP
                     ATLSEQVLASLDQSDRARVETVLSYPEDSAGGIMNTDAITVRPRHSVELVLRYLRLRK
                     DLPEATDALIVVNQRDEYVGMVSLASLITSDPTVTVREVMNTEARSIPADTPDTEVAR
                     LFSEQDLISAPVVDQQGRLIGRITIDDVVDVIIEDADEAVLARAGLDLEDDTFAPIMK
                     SVRGRALWLGVNLLTTFVAAAVINVFEETIAKVVALAVLMPIVASMGGIAGTQTLTLV
                     IRGIAVGQLGRANLPYLLNREFLVAALNGLMLAIVVAVAAAVAFGDTVLGLVIAIAML
                     INLLVAAVSGTLLPSILNALRIDPAIAGGVVLTTITDVTGFFTFLGLATWAYA"
     gene            complement(13725..15071)
                     /gene="pmbA"
                     /locus_tag="AC16QF_06390"
     CDS             complement(13725..15071)
                     /gene="pmbA"
                     /locus_tag="AC16QF_06390"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HIR6448218.1"
                     /translation="MPNELIDLQDSEAGLSSLVGDILDEARRQGADAAEVSVNEDAGL
                     SVSVRKGELETVEFNQDRGFGITVYYGLRKGSASTSDSSLTAIEDTVRAARDIARHTE
                     EDPCNGLADAGLMPDALPELDLYHPWRLDVGEAERLAIACEAAGLSRDERIVNSDGAQ
                     VSTQQSCRVYGNSHGFIGSYLGTRHSVSCVLIAADESGMQRDYWYSLARDPEGLEDME
                     SVGVEAARRVVARLSPRKPLTGRFPVVFSAQMSAGLVGHLVGALSGGSLYRNASFLLD
                     SLGHGVAAPHLSVLEEPHLPGRIGSSGFDGDGVGTSAKHFVEDGVVRSYVLSTYSGRK
                     LGMPTTGNAGGVFNLLLRGRTLAVDDLYRSMGSGLLVTELMGQGVNLVTGDYSRGAAG
                     FWVENGAIAYPVHEVTIAANLKDMLLDVQALGDDVDERGNVRTPSVLVGSMMVAGS"
     gene            15113..>15368
                     /gene="yjgA"
                     /locus_tag="AC16QF_06395"
     CDS             15113..>15368
                     /gene="yjgA"
                     /locus_tag="AC16QF_06395"
                     /inference="COORDINATES: protein motif:HMM:NF016626.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HIR6448219.1"
                     /translation="MTEPPISKSERKRAAARLQEIGRALAELRPARLDEIPLPETLER
                     AIYDYRRFASREAKRRQLQRIGKLMREIDTAPIEAALERLE"
BASE COUNT         2833 a         5171 c         4830 g         2534 t
ORIGIN      
        1 cgaagcgggt cttcatggcc gcccggttta cgatcattcg cgaggagacg tgcgcaatgg
       61 tctcaagagc ttccaggccg ttcgcgcgca aggtgtttcc ggtgtccacg atgtccacga
      121 tgcaatccgc caggttcatg agcggggcga tctccatggc gccgtttagc ttgatgatgg
      181 atacctgacg cccctgacct tcgaagtagc gacgggccac ccgcggaaac ttcgtcgcca
      241 cccgcagctt gccggatccc agatccgcgc ctgcgggagc agccaccatg atgcggcact
      301 tcgcgatgcc caggtccagg cgctcgtagt aatccgcgct gccgccgtgc tccagcaggg
      361 tgtccttgcc cacgatgccc acgtccgccg ccccgtattc cacgtaggtg gggacatcgg
      421 caccacgcat caccaccagg cgatgcccgc cctgccgggt ggcaaaaatg agcttgcggc
      481 tggcggccgg gtcctcctcc gggacgatgc ccgccgcagc cagcattggc agggactcct
      541 cgaggatcct gccccggttg agcgctatgg tcagacccat ttcaattcct ctcgatcagc
      601 ccgaaacccg ctggacctca gcgccgagca gtttgagctt ctcctcgatg gtctcgtagc
      661 cgcggtcgat gtgatagatc ctatcgatga gcgtggtgcc ttccgcaacg agcgcggcga
      721 ccaccaggct gaacgacgct cttaagtccg tggccatgac gggagcggct ttcagcgacg
      781 gcacgccgcg caccgagacg ctggacgggc cgtgcagctc gatgtccgcg ccgagtcgcg
      841 agagctcctg aacgtgcatg aagcggttct cgaagatggt ttccgagacc tcgctggttc
      901 cctcggcgat ggcgttcatc gccaggtact gggcctgcat gtccgtcgga aagccgggat
      961 atggcgccgt ggtgatatcc accgcgcgcg gtcgccggcc gtgcatgtcg agctcgatcc
     1021 agtcgtcgcc aacttcgacc tcggcgccgc tttccacaag cttgtcgagc acggccgtaa
     1081 gggtgtcggg tcgaacgtcg gtgagcctta tcctgccccc ggtggccgca acggcaatca
     1141 ggtaagtccc ggcttcgacg cgatcgggca taacccggta ctcgcatccg tgcaaccgcg
     1201 gcacgccttc gattcttatt tccggggagc cgtgacccga aaccttcgcg cccatggcgt
     1261 tgagaaaatc tccgaggtcg gccacttccg gttcgcaggc ggcgttgcgc agccgcgtga
     1321 ccccctcggc cagcgcggcc gccatcatga ggttctcggt tgcgcccacc gtcaccatgt
     1381 cgaacgcgaa gtcggccccg cggagccggc cgccgacctt gccccgaacg taaccctcgt
     1441 tcacggagat ctccgcaccg agggcttcca ggcctttgac gtgctggtcc acggggcggg
     1501 ccccgatggc gcaaccgccc ggaagggaga cctccgcttc gccgaagcgc gccaacagcg
     1561 gcccgagcac gacgaacgag gcgcgcatgg ttttcaccag ctcgtagggc gcccgcagtt
     1621 gcgccaggct gtcggccgaa acctcgacgc gcatcttctc gtcgacgagc acgcgggcgc
     1681 ccatgcagcc gagcagctcg atcatggtgg tgacgtcctg gagatgaggg acgttgctca
     1741 acgtcaccgg ctcgtcggcc agaagcgtgc ccgccaggat cggcagcgcc gcgttttttg
     1801 cgccagagac ccgcagcgag ccggacagcg tgcgcccgcc cgttatgcgg agcttatcca
     1861 ctgcttcccg ccgcctcctc gggcgtgagg gagcgaatgc tcacggcgtg caggcgaccg
     1921 tcctggatga aagcgtcgat gcatccgtaa acggtctgtt gcttgcgaac gcgcgtcatg
     1981 ccctcgaaca tccggctgac cacggtgatc atcgcgcggt tcccctcgac gacgacgtcc
     2041 acgcgcaagg cgtcgtcctc cccggacagc ctttctgtga tgctcgccgc gatttcttcc
     2101 tgcatgtccc gccctgccgc agtcggttaa aggttcagcc tgccgcgtcc cgggaaagcg
     2161 gcagaacttc catcagcccg gaaatttcaa tgatgctgag caaatcctgg gggacgccag
     2221 cataaaccac ctcctgcccc cgcgcatggg caaagcgaaa ccaggtcatc agaagggcga
     2281 cggctatgct gttacagctc tccaggccgg aaagatcgac gacggctttc tcgtcgcgcc
     2341 gttcggcgag aaactgctcg ccagccgccc tgaccgcagc gaagttgtcg acgccgacgc
     2401 gaggcggcag ggaaaacgcg ctcaacgatc gtcaccagct ccgccggccc cgtcgctcga
     2461 cggcgcctcg tctgacccgc ccgagtcagc cgacgcggca cccggtgtct cgttggccag
     2521 gagcaaagtc caggcgtcga tgaccttgtc caggtccccc ccgtatctct gatcctgcac
     2581 ggcgctggca aactggctgc ggtaggtcag accgatgttc acgccgttga cgatgatgtt
     2641 ccccatgcgc caggcgccgt ccttgccctg cgccattgag taaaccaccg ggtatacatc
     2701 tccggtgttc atgcggattt ccatcttcac gctctcgcgg cgggggcttt ggggcggccg
     2761 ctcggagggc acgatgacca cctcgccgtc cctgaactcc gtcagcgcca gcgcgtaggt
     2821 tcgaaccagg ccccatttga aggtatcggc gaagcgatcg cgctgctcgg gagtggcgga
     2881 tttgtagtgc acggccatga cgctgcgggc aaaccgcttg aagtcgacca ccggcgcgag
     2941 cagcgactcc acctcgccgt agaaccgttc cgggtcatcc tcgacgtacg gtctggatgc
     3001 atcgatgagc gcgagcatct cgtccgacgt gcgctccacc acctcggtgg cggtacgggt
     3061 cgcgacgtcc gagggctcca tggcgcgagc gggcgcgagg cctgcgagca gcagggcggc
     3121 gatcgcgagc acgaagccgg attcaagacc cgagtgattc atcaaacgca tcgtcatcag
     3181 cctgctccaa gccgcgttat gaggtcgccg accaggttct ccagcacgag ggcgccctgg
     3241 gtatcgtaaa tcagatcgcc gtccgccagc gtctcgtcat ctgcccccgg cagcagattc
     3301 acgtagcgcg caccgagaat tccttcggtg agtatctgcg cccccgtgtc ggcggaaagg
     3361 ccgcccggac tgccgtcgat ttcgagggtc accacggcct ggccgtagat ggtgtcgacc
     3421 tcgatgagcg ccacgcgccc gatctggaca ccggcaatgc tcaccttggc acgctcgcgc
     3481 aggccggcca cgtcgtcgaa ccgcgccacc aactgatagc tgccacgatc tgcatccaga
     3541 ctgatgccgc tgaccttcac ggcgagaaag acgagcgcaa ggattccagc aagcatgaac
     3601 gcgccaacgc tgatctctag ggttcgtatg tgcatcagtt gaactctccg tacatgctca
     3661 gcgtgagcaa gaagtcgatt gcgagcacca tcaccgagga ggccacgacc gtcctggtag
     3721 tcgccaggcc gattccctca gccgtcggtt cggtatcgta cccctgaaaa acggcgatcc
     3781 aagtgacgac caccgcaaac acgacgctct tcagcatgcc tttgccgacg tcgtgccata
     3841 gctccaccgc gtcctgcata ccggaccaga agccgccggg atcgccgccc agccactcca
     3901 cggcgaccac gaggccaccg tagatggcca cgacgttgaa gatggcggtg agcagaggca
     3961 tgctgatgac cccggcccaa agccgcggcg ccacgatccg gcgcagcgga tcgacgccca
     4021 tcatctccat gctcgacagc tgctcggtgg ttttcatgag cccgatctcc gccgtgatgg
     4081 cggaacctgc ccgaccggcg aacagcagcc cggtcaccac cggcccgagc tcgcgcagca
     4141 gcaccagcgc gagcccgaga cccaccgagt cctcggcacc gaaacgcgcc agctgggtgt
     4201 gaaactgcag agcgatcacc gcaccgatgg agaacgccga aagaacgatg atgatgaggg
     4261 acagcacgcc cacgtggtag atctggcgaa cgagcaggcg caggtccccg ggccccggga
     4321 gccccgcgag cgccgtgaac cacatgatgg tcgccctgcc gaggctcgcc atccggtcca
     4381 ggctgcggcc accgaagctg cgcaggaact ggatcacgcg ttcacgtccc ctagaacgtc
     4441 gtagaggaac tcgtcggcag gatagtgaaa gggcacgggg ccgtccggcg agccgtgcat
     4501 gaactggtgg acgcgtggcg agtcgtccac cgccagagta tcgggcgtgc cggatcccat
     4561 gatcgtgccg tcggcgatga gatagatctg gtcggcaatg ctgatggtct cgtgaatgtc
     4621 gtgagaaacc acgaggctcg tcgtcttcag cgagtcgttg aggctctcga tgaggctgag
     4681 cagcaccccc atggcgatcg gatcctgccc ggtgaagggt tcgtcgtaca tcaggaggtt
     4741 cggatccagc gcgacggcac gtgccagggc gacccgccgc gacatgccac cggaaagctg
     4801 gctcggcatg agatctctcg cgccgcgcag ccctaccgcg ttcagcttca tcagaacgag
     4861 gtcgcgtacg gtgtcctcgg ggagatcggt gtgaacgcgc aggggaaacg ccacgttctc
     4921 gaagacgttc aggtcggtga acagcgcgga agactggaac agcatcccca tgtccttacg
     4981 cagctcgaac agctcgcgtc ggctcatccc gcagacgtcc tgaccatcga tgagcacgac
     5041 cccgctgtcg gggcgcacct ggcctccgat gattcgcagc agggtcgtct tgcccgtgcc
     5101 gctcgggccc atgatcgcag tgatcttgcc gcggggcacc gacaggctaa gatcatcgaa
     5161 aatgacccgg tcaccgcgga cgaaagagag attgcggatc tcaatgaggt tgtcgtcggc
     5221 gccgttcatg aacgtctggg gtcgtgagca agacgggcgc aactataaca tcgcgggctg
     5281 gagttggctg acgggcgcga tgagactaaa gtggccaact tgactattga tccccataac
     5341 ccggccggcg ccagctcatg ctgctctcga tagccgccct catcgttggc ttcatggcac
     5401 tgctgtggag cgccgatcgc tttctcgccg gtgcggcaag caccgccggg cacctcggtg
     5461 tgtccaagat gctcatcggc ctcacggtgg tctccctcgg cacgtcggcc cccgagatac
     5521 tcgtggccct gacggctgcg gtggaaggca acccgctgct cgccgtgggc aacgccatcg
     5581 gctcgaacat cgcaaacatc ggcctggtgc tgggcatcac cgcgatcatc gtgccgctgc
     5641 ccttttccca gaccgtgctg cgcgccgagc tgccctggct gctgggcgcc acctttctgg
     5701 ccctggctct tctcttcgat ctcgagatgt ccgcattgga ctcggtgctg ctgctggccg
     5761 ggctgggcgt gattctgtgg cgcctggtgc gcagtcaaaa gaccgcggga agcgacctgg
     5821 cagaaagcat caccgaggag ctggaggatc ttccgcagat gtcgcgcggc caggcagccg
     5881 tgtggctgct gatcgggttg ctgatcctgc tggccagcgc cgaggtgctg gtctgggccg
     5941 ccagcgacat cgccgaccgg ctgggcgtca acgacatcat catcggcctg acaatcgtcg
     6001 ccatcggcac cagccttcca gagcttgcgg caacggtggg ctccgcgctg aaaggccaca
     6061 cggacatcgc catcggcaac gtcgtgggat ccaacattct gaacatcctg gcggtgctgg
     6121 ccgtccccag cctggtggac ccgatcctca tcgagaacgt ctggctgtgg cgggacggcg
     6181 gcatgatgct ggcgctgacg ctcctgctcg cggtgttcgc ctacgccgtc gggtcccagc
     6241 ccgtgatcac ccgcttcgag ggcacggtca tgctgtgcgc ctgggtcggc tacaacctgc
     6301 tgctcgtgtc tcaggcacag ctgggctgat ggctttcccg gcgccggtgc tcgagcgcgc
     6361 ggcggatatc gagctggtgg ctttcgatgt ggacggcgtg ctcaccgacg gcaagctcta
     6421 ctacagcgac gacggtcgcg agctgaaggc cttccatgtc caggacggcg ccgctttgaa
     6481 gctcctgctt tcccacggca tcgtggtggc gatcatcacg ggccggtctt ccagcatcgt
     6541 cacgagacga gccgacgagc tgggcatcgc ccacgtctac cagggggtcg cggacaaggc
     6601 cgccgtcctg gcggccctgg ccgagacggt cggcaccacc ctcgagcgag ctgcctatgt
     6661 cggcgacgat ctgccggacc tcggcgtgtt ctcccgggcc ggaatggcca tcagcgttcc
     6721 caacgcgcac ccggccgcgg cggccgccgc cgactacgtc accctggccc ccgggggtgc
     6781 aggggcggcg cgggagatcg cccagctttt cctggaagcc cggggcgagt ggccctacgg
     6841 gtaaccggct gagccatgcg caagcgctcc aaatgggaac gatggtcgac gtggacggcc
     6901 atcctgggtg ttgcggtggc cggggtcgtt ctgctgagcg agcccctgga gccggagaca
     6961 gaagcggaga ccccgcccgc cggcatcgcc gaagagccgg atctttacgt cgaagacgcc
     7021 gtcatcaccc agtacctgcc aagcggaacc gtccactacc ggctggtgtc gaaattgctg
     7081 aggcagttcg aggcggacga gctggtgcgg ctgaccaagc cgaacctggt ggtcaacagc
     7141 gaaacgcgct cgccgtggca agtgcagtcg gagcacggct acgtgcgccg aatgggcgga
     7201 cccgagcgtg gcggcgaagg cgaagaggtg gtttttctgc gcgaggacgt ccagctagag
     7261 cagcgggaac ccgacggtcg ccacatacgc ctgaccacgg agaccctcta tgtgtatccg
     7321 gagcgagagt acgccgagac tgaccgcgat gttatgatcg acacccatgc cggccggacc
     7381 acggccaccg gactcaaagg tgacttgcag cgtggattgc tacagctgtc ctcctcagat
     7441 cgacgccgcg tgcacacgat tctgctgccg gatcagttca agtaggctgc tgcgtgccgt
     7501 gacgcttggc gccgtcctgc tcgctccggg cgcgctcgct ctgcccgagg acgccgacca
     7561 gcccattcac atacgcgcca acagcgcgga gatggatcag gaacaggggc tggtgatcta
     7621 tcgcggcgac gttcaggtgg accaggggac tctgcgcgtc atggccgacg agatgaccgt
     7681 ggagtacgag gatcaggagg tgatacgcat caccgccaag ggagagcccg cccgctacca
     7741 gcagcagatc gaggttgacg aggaagtggt tcgcgctcgg tcgtcgacca tcatctacca
     7801 cacgcgcgac gagcgcatcg acctggtcgg caacgcgttt ctcacccagg aaggcaacga
     7861 gatcaccggt gacctcatcg agtacgacat cgtcgccggc aaggtggatg cgaaggcgga
     7921 aagcgaaacg ccggtgcgga tgatccttca gcccgctcgc aagaaacccg acggcgggta
     7981 aacggcccca tgcccacact ggaagttcgc tctctacgca aacagtaccg taacaaggtc
     8041 gcggtggaag acgtcagcct gcacgtcagc accggcgaga tcatcggcct gctgggaccc
     8101 aacggtgccg gcaaaaccac caccttctac atgatcgtcg gcctgctgcg agccgacggc
     8161 ggcgatatct ttctcgacga gcagcggatc acccggaccc ccatgcacgt gcgcgcccgg
     8221 gcgggtatcg gctatctgcc ccaggaagca agcgtcttca gaaagctcag cacggaggac
     8281 aacgtgcggg ccatcctgga gctgagacgg gacctgaacc ggacccaacg ccaggccatg
     8341 ctcgatcagc tgatcgagga gtttcacctg ggcggggtgc gcgagtccct cggcgagcgg
     8401 ctctccggcg gcgagcgacg ccggctggag attgcccggg cactcgccat ggagcccagc
     8461 ttcatgctgc tcgacgagcc gttcgcgggc gtcgacccca tttccgtcgg cgacatcaaa
     8521 aaggagatcc gcgatctggc ggcccgcggc atcggcgtgc tcatcaccga tcacaacgtg
     8581 cgggacaccc tggacatttg tgaccgggcc tacatcgttc atgagggcca catcattgca
     8641 gaaggcgatt ctggcgccat acttgcagac gagaaggtca aaaacgtata tctgggcgag
     8701 agcttctctc tttgagcctg gctccttgag atcagccctc agttccaggc cgcgatagcg
     8761 cggccgcaca gccaggggcg agcgtcaggc acgccaagcc ttttgacccg ttaggacaac
     8821 aaccggcaac catgccgcgc ggaaggacga ccgctcacca gagccctcgg tttgcatgaa
     8881 acagactctg tcgctcaagc tcggacagca gctcaccatg acgccgcagc tgcagcaggc
     8941 catccggctg ctgcagcttt cgtgcctgga cctgcgtcag gaaattcagg atgccctgga
     9001 aggcaacccg atgctcgaac tcgacgagga ggacgggggc gacgagccga cgggctctcc
     9061 cgcagaggcc gatcgggccg aaagcgacgg tgacgacgac ctcgacggcg tggatgcgac
     9121 cctggaaggg gacgttatcg acgatctgcc ggtcgacacg agctgggacg agatctacca
     9181 gcccgcgcct gctcccgctc cggcagccgg cgattccgaa tcctgggatt tcgaaacgcg
     9241 gaacgcgccc gaggagaccc tgcaggacca tctgctctgg cagctgaacc tcacgcccat
     9301 gtccgactcc gatcgggtga tcgccatggc gatcatcgac accatcagtc cggacgggat
     9361 gctgaccacg cccgtggaag agctggtgac gtctctggaa ggcgtggtgg acgaggaggc
     9421 ggagattgcc cacgccgagg tcatggccgt gctcaagcag atccagcatt tcgaccccat
     9481 cggggtgggc gcccgggacc tgggcgagtg cctgaggatc cagctcgagc agctgccggc
     9541 gggcacgccg tggctcgcgg agtcgctgac cctcgtcaac gagcacctgc ctctgctggc
     9601 cagccgggac ttcgccgccc tcaaacgtcg gaccaagctc ggcgaagagg aactcggcga
     9661 ggtcgtaagg ctcattcaga cgctcaatcc gcgcccgggc accacggtgg cccaggaggc
     9721 ggcagagtac gtcgtccccg acgtcatggt atccaggaaa gaggggcgct ggctggtgga
     9781 gctcaacccc gacgcgacgc cgggcttgag aatcaacgcc ggctacgccg ggctcgtcaa
     9841 gcgcgccgac aacagcgccg acaacacctt cctgcgcgat catctgcagg aagcgcgatg
     9901 gttcctcaag agcctgcaga gccgcaacga gaccctgctc aaggtcgcca cccgcatagt
     9961 ggaacatcag cggggattcc tggaatacgg ggaagaagcc atgaagccac tggtgctcaa
    10021 cgagatcgcc gaggccgtgg aaatgcacga atcgaccatc tcccgggtca ctagccgcaa
    10081 gtacatgcac acgccccggg gcatcttcga gctgaaatat ttcttttcca gccacgtgtc
    10141 cacgagcagc ggcggcgagg tttccagcac ggccatccgc gcgctcatca agaagctgac
    10201 ggccgatgaa aatccaagaa aaccgttgag cgacagccgc atcgctacga tactggcgga
    10261 gcagaacatc aacgtcgcca gacgcaccgt ggccaaatat cgagagtcga tggccatacc
    10321 accgtccaac gaacgcaagc gtctggtttg atcattcatg acggcacacg acgcgactcg
    10381 acatcaacag caaaacaagg agccagcatg cagttgagca tctccggtca tcacatcgac
    10441 gtcaccgagg cgctgcggaa ctacgtgacc gaaaaaatgc agaaactgga acgccacttc
    10501 gaccacatca ccaacgtcca cgtggttctg agcgtcgaga agctgcgcca tcgcgccgag
    10561 gcgaccatac acgcgagcgg caccgaggtg ttcgccgact gcgattgcga cgacctctac
    10621 gcggcaatcg acgggctcac ggacaaactc gaccgtcagg tcctcaagca caaggagaag
    10681 ctgctggacc gacaacaacg ccggggacgc tgagcggggg ccatgccgga catcaccacc
    10741 atactcagac cggattgcac gagagcttcc gtcgaggcgc cttcgcgcaa gcgcgctttg
    10801 gaggcggccg cagacctggt ggccaagcat catccagatc tgtccgcgcg caagctgttc
    10861 gacgagctca tggcgcgcga gcggctcggc agcacggggc tcggcgacgg cgtcgccatc
    10921 ccccactgca ggattccgtg cgagcagatc accggcgccc tgctccacct gcgagaggcc
    10981 gtggactacg agtccatgga cgacgagccg gtggatctgc tgtttgtgct cgccgtgccg
    11041 cccgaggaaa cgacggccca tctggacctg ctggccgccc tggcgcggat tttcggcgat
    11101 ccggaaaatc gcagacgcct gcgcagcagc gcaacggatg aagccctcta tcaggaactg
    11161 gtggggctct tcagcagtca ggcggcatga agctcatcgt catcagcggc cggtccgggt
    11221 cgggcaagag cacggccctg cgagcgctgg aggattccgg ctacaactgc atcgacaact
    11281 tccccgtcga cctgctcccc cacctgatcg cgaacgccac acgggccgtc gagccgcagc
    11341 cggacctggc cgtgtgcatc gatgcccgca atccggacct ggaagccttt ccccagatca
    11401 tcgagtcgct gcgctccccc agcgtggact gcgaggtgat cttccttgac gcccagagcg
    11461 cgacgctggt caaacgcttc agcgagaccc gccggcgaca cccgctgacc ggcacgggta
    11521 aggacctcct ccaggccatc gaatcggagg atgcgatcct gcaaccgatt gccggcctgg
    11581 cggatctcag catcgacacc accaccatga gaggcagtgc cctgaccgag ctcgtgcgca
    11641 gccgcatcgc ccacaagcgc agcaactcgc tctctcttct gttccgatca ttcgggttca
    11701 agttcggtgt gcctgtggac gccgacttcg tcttcgacat ccgctgcctg cccaatccct
    11761 actgggacga gtccctgcgt ccgctgaccg gcaaggacgg ggcggtggcg tcgtttctgg
    11821 aagcggaagc ggccgttggc cagatgttct cggacatccg gtcgttcctg gaacgctgga
    11881 ttgccgcctt cgagaaagac aaccgggcgt acatgaacat cgccctgggc tgcaccggcg
    11941 gccagcaccg cagcgtgtac atggcggaaa agcttgccgc ccacttctcg gcccagcggc
    12001 ccaacgtgct ggtcaggcac cgcgagctgg caggttcgta accccgccat gcgggaggga
    12061 aggatcacca tagtcaaccg tctcgggctg cacgcccggg ccgcggccaa gttcgtcaat
    12121 ctggcaaaga ccttctcgag ccaggtggag ttgacgaggg acgccgagcg cgtggacggc
    12181 aaaagcatca tgagcgtcat gctgctcgcc gcgcccatcg gcaccgagct gatcctgcac
    12241 gtcgtcgggc cggacgagga cgaggccttc gaagccctgg agcaactcgt cgacgaccgc
    12301 ttcggagaag ctgagtgaac ggccggcgca tctggtaatc tgcgcgcgcc ctcaagtttc
    12361 ggtacccgcg tggactttca ggaacagcta agcgaagtca tccgccaata cggcagcgga
    12421 agtctggacg agaccagatt tctgctcgaa tccctgcgcg cgccggaaat cgccaacctc
    12481 atggagtcca ccccgcccag agtccgccgg gtcatctggg ggctcctgga cgaagaggac
    12541 gagaaccagg tcattcagca tctccgcgag gacgttcggg ccgattttct ggagtccatg
    12601 aacaccgagc ggctggtggc cgccggcgtc gacctggagg tcgacgactt cgcggacatc
    12661 ctccagcagc tgccggcaac cctttccgag caggtcctgg catccctcga tcagagcgac
    12721 cgggcccggg tggagaccgt gctctcctat ccggaggaca gtgccggcgg catcatgaac
    12781 accgacgcga tcaccgtgcg gccacggcac tccgtggagc tggtgctgcg ctacctgcgc
    12841 ctgcgaaagg acctgccgga ggccaccgac gcgctcatcg tcgtgaacca gcgggacgag
    12901 tacgtcggta tggtgtcgct ggccagcctg atcacctcgg accccaccgt aaccgttcgc
    12961 gaggtgatga ataccgaagc gcggtcgatc cccgccgaca cgccggacac cgaggttgcg
    13021 cgcctgttct ccgagcagga tctgatctcc gcgcccgtcg tggatcagca agggcgcctg
    13081 atcggccgga tcaccatcga cgacgtcgtc gacgtcatca tcgaggacgc cgatgaagcg
    13141 gtgctcgccc gggccggtct ggacctggag gacgacacct tcgcgcccat catgaagtcc
    13201 gtgcgcggcc gcgcactgtg gctcggcgtc aacctgctga ccaccttcgt ggccgcggcg
    13261 gtcatcaacg tgttcgagga aaccatcgcc aaggtggtgg cgctggccgt gctgatgccc
    13321 atcgtcgcca gcatgggtgg catcgcgggc acgcagacgc tgacgctggt cattcgcggc
    13381 attgccgtgg ggcagctggg tcgcgccaac ctgccctatc tgctcaatcg ggaatttctg
    13441 gtcgccgcgc tgaacggctt gatgctcgcc atcgtggtag ccgttgccgc cgctgtcgcc
    13501 ttcggcgaca ccgtgctggg cctggtcatc gccatcgcca tgctgatcaa cctgctggtc
    13561 gccgccgtgt cgggaaccct gttgcccagc attctcaacg ccctgcggat cgatccggcc
    13621 atcgccggcg gcgtggtgct gaccacgatc accgacgtga ccggattttt cacctttctc
    13681 ggtctcgcga cctgggccta cgcctgacct ggcgacaccc cggtttacga ccccgcgacc
    13741 atcatggagc cgacgagcac gctgggcgtg cgcacgttcc cgcgttcgtc cacgtcgtcg
    13801 cccagcgcct gcacgtccag cagcatgtcc ttgaggttgg cggcaatggt gacctcgtgc
    13861 accggatagg cgatcgcccc gttctctacc cagaaccccg cggcaccccg cgagtagtca
    13921 ccagttacca ggttgacgcc ctgacccatc agctccgtga ccaggagccc ggagcccatg
    13981 gagcggtaaa ggtcgtccac ggccagcgtt cggcccctca gcagcaggtt gaaaacaccc
    14041 ccggcgttgc ccgtcgtggg catgccgagc ttccgacccg agtaggtgga caggacgtag
    14101 ctgcgcacga cgccgtcctc cacgaaatgc ttcgccgagg tgcccacccc gtcaccgtcg
    14161 aaacccgagc tgccgatgcg gccgggcagg tggggttcct cgaggacgct caggtgaggc
    14221 gcggcgacgc cgtggcccag cgaatcgagg agaaacgacg cgttccggta gagactccca
    14281 ccgctcaacg ccccgaccag gtgccccacc agacccgccg acatctgcgc cgagaagacg
    14341 acgggaaagc ggccggtcag cggcttgcgg ggcgacagcc gtgccaccac cctgcgggcg
    14401 gcttcgacgc ccacgctctc catgtcctcc agcccctccg gatcccgagc caggctgtac
    14461 cagtaatcac gctgcatgcc cgattcatcg gccgcaatca gcacacagct gacgctgtgt
    14521 ctggttccga ggtagctgcc gatgaacccg tgactgttgc cgtagacccg acacgactgc
    14581 tgcgtgctca cctgcgcccc gtcggagttc acgatgcgct cgtcgcggct gagcccggcc
    14641 gcctcgcagg cgatcgccag ccgctcggcc tcgccgacat ccaagcgcca ggggtgatac
    14701 agatccagct ccggcagcgc atccggcatc aaaccggcgt ccgccaggcc gttgcagggg
    14761 tcctcctcgg tgtggcgggc aatgtcccgg gccgcgcgca cggtgtcctc gatggccgtc
    14821 aggctgctgt ccgacgtgct cgccgagccc ttgcgcaaac cgtagtagac ggtgattcca
    14881 aatccccggt cctggttgaa ctcgacggtt tccagctcac ctttgcgcac cgaaacggaa
    14941 aggccggcgt cctcgttcac ggacacctcg gcggcgtccg cgccctggcg acgggcttcg
    15001 tccaggatgt cgccgaccag ggacgaaagc cccgcctcgc tgtcttgcag gtcaatcaac
    15061 tcgttcggca tggagtcctc acatttgcct ctgcttcggg tacgctcgac cgatgactga
    15121 gcctcccatc agcaaatccg aacgcaaacg cgccgcagcc cgcctgcagg agatcggccg
    15181 ggccctggcc gagctgcgtc ccgccaggct ggacgagatc cccttgccgg aaacgctcga
    15241 gcgcgccatc tacgactacc ggcgatttgc ctcccgggag gccaaaagac gccagctcca
    15301 gcgcatcggc aagctcatgc gtgagatcga caccgccccg atcgaggcgg ccctggagcg
    15361 tctcgagg
//
LOCUS       DBNHLM010000136        18481 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1335696, whole genome shotgun sequence.
ACCESSION   DBNHLM010000136 DBNHLM010000000
VERSION     DBNHLM010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 18481)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18481)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18481
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1335696"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /gene="cysG"
                     /locus_tag="AC16QF_06400"
     CDS             complement(<1..959)
                     /gene="cysG"
                     /locus_tag="AC16QF_06400"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF007922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HIR6448220.1"
                     /translation="MDYLPLFLDLKQRPCLVVGGGEVARRKIELLIRAGAAIFIVSPE
                     LSEATRALVDERGLDVVPRRFRPSDVEGHYLVVAATNDPDVNRIVYDMCVSRRILVNT
                     VDQPELCSAIFPSIVDRSPVVVAVSTGGSSPTLARVVRGWLEARLPARLGELAGFVKE
                     RRERVKSVLGSESERQAFWEALIQGPVAEQVLTGRDEEAAAAFDELLPAAAETARRGS
                     VALVGAGPGDPELLTLKGLRLLQAADVILYDNLANQRLLDYARRDAEKIYVGKKRKFP
                     GIRQEGINALLVEHALAGRHVVRLKGGDPFIFGRGGEETETLA"
     gene            complement(978..2267)
                     /gene="serS"
                     /locus_tag="AC16QF_06405"
     CDS             complement(978..2267)
                     /gene="serS"
                     /locus_tag="AC16QF_06405"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR6448221.1"
                     /translation="MLDIKVLRDDPESVRDRLAVKGYLLDVSRVQALESERKRLQELT
                     ETLQNERNTRSKAIGKAKAAGEDIAPLLAEIGDLGDRLDAAKADFDALQETYQGFLRE
                     IPNLPDASVPAGNDENDNQEIGQWGERRSFEFEPADHVALTEAGGLDFDTAAKITGSR
                     FVVMHGPIARLHRALVQFMMDVHVIEHGYREVYVPYIVNAESLMGTGQLPKFAEDQFR
                     LEGSNEFYLVPTAEVPVTNIYRDTILAADQLPVRHVCHTPCFRSEAGSYGKDTRGMIR
                     QHQFEKVELVMLVRPGDSWRALEELTDHAETILRKLELPYRSVVLCGGDLGFSAAKTI
                     DLEVWLPGQDCYREISSCSNFLDFQARRMLARYRHPESGKPELVHTLNGSGLAVGRTL
                     IAILENYQDGEGRVAVPDALRDYLAGAESLDLSAGAG"
     gene            complement(2294..2668)
                     /gene="crcB"
                     /locus_tag="AC16QF_06410"
     CDS             complement(2294..2668)
                     /gene="crcB"
                     /locus_tag="AC16QF_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HIR6448222.1"
                     /translation="MTANALVWVALGGALGAVGRFLIGTWLFVPGQFPWATLTINIAG
                     SLGIGLLWGLGHQQPWFEQWGRYLIVVGLLGGFTTFSAFSLETVGLVEGQRWGGAVVY
                     VVASVVGCIVAAWAGQRLVGYY"
     gene            complement(2665..3954)
                     /locus_tag="AC16QF_06415"
     CDS             complement(2665..3954)
                     /locus_tag="AC16QF_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR6448223.1"
                     /translation="MSLGDENAPLAERLRPNCLEEFVGQAHLLGAGKPLRTLAESGRL
                     HSMLLWGPPGTGKTTLAKLLAARAGARWISLSAVLSGVKDVRAAVAEAEALRGLGETT
                     VLFVDEVHRFNKAQQDAFLPHVEKGTVTFIGATTENPSFEVNAALLSRARVYVLKPLG
                     EEDLNRVIDRAAEQALNLPVTPDARALLTSLADGDARRMLNLLEVAAQLAEKEVDEAV
                     VTEAAGSRYRRFDKGGDAFYDQISALHKSVRGSAPDAALYWLCRMLDGGCDPLYVARR
                     VVRMATEDVGNADPRALQIALAAWDTYERLGSPEGELALAQAVLYLASVPKSNAAYVA
                     YGRAMGFVREGASEAVPMHLRNAPTRLMKDLGHGRDYRYAHDEPEHYAAGETYWPDGL
                     DPQAFYEPSDQGLEAKIRERLSRLRRLDQNTSGRAGR"
     gene            complement(3938..4567)
                     /gene="lolA"
                     /locus_tag="AC16QF_06420"
     CDS             complement(3938..4567)
                     /gene="lolA"
                     /locus_tag="AC16QF_06420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein periplasmic chaperone
                     LolA"
                     /protein_id="HIR6448224.1"
                     /translation="MKSWFRIATDPVVWLVAALSCASAAADPRQALSERLAAMDVFVA
                     EFSQEIRGSQGQVLERSTGRFVLDRPLFKWEVDHPYPQTVVTDGDRLKVYDADLEQLT
                     IRPLAEALKDTPVSLLTREGVALGNEFQVVEVTDELGASYILTPNAPDTLYAQIRLHF
                     SDNALAALAITDHLGQFTEILFSTSAEPPVIQSSEFQLVVPPGTDVIGG"
     gene            complement(4539..6791)
                     /locus_tag="AC16QF_06425"
     CDS             complement(4539..6791)
                     /locus_tag="AC16QF_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR6448225.1"
                     /translation="MTTSTIPVREIGLIGFTAAALYTMLAMVSYSPADPAFSFTGTGG
                     SVDNLVGRSGAWFADFTLYLFGRVAYILPVALVVIGVRLFRERSETFSWWLLALRCAG
                     WLAVLIFGCILARIHFAPAAALPGGAGGILGEALVALGLPVFGWIGLTLIALTAVMIG
                     AQAAAGFSWLSVAELTGRWVHAFTANAVLWLDRQLDRWRRFREARSGAKEMMRAREEA
                     LGDERAKRGSRAKPTIKPAAAQKASTVAVQEPLFESTPSGDLPGLDLFDERQPGDDRG
                     YSDESLEAISRLLELKLKDFGVEAEVVSVLPGPVVTRFEIQPAAGVKVQKISSLVKDL
                     ARSLAVISVRIVEVIPGKSVVGIEIPNEHREVVRLKEVFSSAVFQDSKSPLTLALGKD
                     IAGESVVADISRMPHLLVAGTTGSGKSVGVNAMLLSILCKATPEDVRMILVDPKMLEL
                     SVYEGIPHLLTPVVTDMKEAAHALNWCVAEMERRYKLMAALGVRNLAGYNRQIADAAK
                     KGQTISDPLWDADSEGEPAALGKLPLIVVFIDEFADMMMIVGKKVEQLIARIAQKARA
                     AGIHLVLATQRPSVDVITGLIKANIPSRISFQVSSKVDSRTILDQGGAEQLLGHGDML
                     YLPPGSGLPTRVHGAFVSDEEVHRVVSDWKQRGEPDYDNGVLSGGDDEPFLSIEPDSG
                     SAEQEDALYDEAVAFVLESRRASISSVQRKLRIGYNRAARLIEAMEAAGVVSAMNSNG
                     SREVLVPNRD"
     gene            6957..7256
                     /locus_tag="AC16QF_06430"
     CDS             6957..7256
                     /locus_tag="AC16QF_06430"
                     /inference="COORDINATES: protein motif:HMM:NF038106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4642 family protein"
                     /protein_id="HIR6448226.1"
                     /translation="MRPDKTKVVDEVWDDDRIEGFLHKAPLGDERSADYSALLYAYRS
                     MRPDDFATFIRLFVDAGRDVNAKSNGGETLLATIRDHRHGAPFRAILEKAGGSAS"
     gene            7275..7979
                     /gene="aat"
                     /locus_tag="AC16QF_06435"
     CDS             7275..7979
                     /gene="aat"
                     /locus_tag="AC16QF_06435"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011533432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HIR6448227.1"
                     /translation="MSGLSVLSSHRVGFPPPESALAEPNGLLAVGGSLTPEWLLAAYA
                     RGIFPWFDDDASPLLWWSPDPRAVLLPEELRVTRSLAKRIRNAGFNVTMDQAFADVVA
                     ACAAPRGAEAGTWITADMARAYAELHEMGFAHSVETRLDGELVGGLYGVSLGRMFFGE
                     SMFARQRDASKVALYHLTLRARDWNFSLIDCQIMNEHLRSLGAREMPRAEFLRLVHEN
                     RAEPTRRGSWADETAR"
     gene            7976..8686
                     /locus_tag="AC16QF_06440"
     CDS             7976..8686
                     /locus_tag="AC16QF_06440"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003108766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HIR6448228.1"
                     /translation="MKDETADKQFFITPAHPCSYLPKRNAQTLFLDPRETITPQTYQQ
                     LTEQGFRRSGGHLYRPHCKTCQACVPTRLPVAEFVARRSQRRTLARNTDLAVRVEPAA
                     YSPAHYSLYASYIAGRHGDGDMYPPSPDQFRSFLLSQWSDTLFVNSFLDGKLVAVAVT
                     DKLDDGLSAIYTFFDPALEQRSLGVQSILQQIELARELGLSYLYLGYWIRDAEKMRYK
                     TDYRPTELFVNGRWVRIT"
     gene            8768..8983
                     /gene="infA"
                     /locus_tag="AC16QF_06445"
     CDS             8768..8983
                     /gene="infA"
                     /locus_tag="AC16QF_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR6448229.1"
                     /translation="MAKEEQIEMEGTVIDTLPNTMFRVELDNGHVVTAHISGKMRKNY
                     IRILTGDKVKVELTPYDLSKGRITFRK"
     gene            complement(8987..11278)
                     /gene="clpA"
                     /locus_tag="AC16QF_06450"
     CDS             complement(8987..11278)
                     /gene="clpA"
                     /locus_tag="AC16QF_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HIR6448230.1"
                     /translation="MLSKDLEVTLNEAFRAAKAKRHEFMTVEHLLLALLDNKVALDVL
                     EKVGVDVDRLRKDLSEYIESTTPLIPAGDSDRETQPTLGFQRVLQRAVFHVQSSGRKE
                     VTGGNVLVAIFSEQESQAVFFLKQQNVARIDVVNYIAHGISKVFGEGESGEPAAEQEE
                     EGAGEEAQQSALEAFATNLNDQARNGRIDPLVGRDAELERVIQVLCRRRKNNPLLVGE
                     SGVGKTAIAEGLAKRIVDGQVPEVVSDSTIYALDMGALLAGTKYRGDFEKRFKLVLAE
                     LKKQEGSILFIDEVHTIIGAGAASGGVMDASNLLKPLLTSGDIRCMGSTTYQEYRGIF
                     DKDRALSRRFQKIDITEPDVNDTYNILKGLKSRFEDHYHIRYTDKALRAAAELADRYI
                     TDRFQPDKAIDVIDEAGAAQLLLAPSKRKKSIGATDVEQVVAKIARIPSTQVTSSDKE
                     ALRNLDAKLKMVVFGQDEAIDSLASAIKLSRAGLKSGEKPIGSFLFAGPTGVGKTEVC
                     RQLANIMGVELLRYDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEAVTKHPHSIVLL
                     DEIEKAHPEVFNLLLQVMDHGTLTDNNGRKADFRNVVLVMTTNAGAQDMSRPSIGFTQ
                     QDHTSDAMEAIKKLFTPEFRNRLDGIIQFHSLPKEVIKTVVDKFLIELQAQLDDKKVT
                     LEVDDAAREWLVVEGYDEKMGARPMQRLIQERIKRQLAEDVLFGALSKNGGTVYVTVK
                     DGDLHLRCEAPEGSADKTPAATT"
     gene            complement(11312..11704)
                     /gene="clpS"
                     /locus_tag="AC16QF_06455"
     CDS             complement(11312..11704)
                     /gene="clpS"
                     /locus_tag="AC16QF_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017641761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HIR6448231.1"
                     /translation="MEPTNRSQIPPRVLKSDPLQAQSGEGDADDSSDSGLATVTAKPE
                     LKRPPMYKVVLLNDDYTPMEFVVHVLEIFFAMDREKATQIMLTVHTQGAAVVGIYPRD
                     IAETKSEQVNQYAQENQHPLISTIEMTD"
     gene            11941..12261
                     /gene="cspD"
                     /locus_tag="AC16QF_06460"
     CDS             11941..12261
                     /gene="cspD"
                     /locus_tag="AC16QF_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein CspD"
                     /protein_id="HIR6448232.1"
                     /translation="MAMGKVKWFNNAKGYGFILPDGGGDDLFAHYSSIQMDGYKTLKA
                     GQDVEFDILEGPKGLHAINIRAEAIESEDSGEDAPVDESAAADPGPAEASPPARETSR
                     EATS"
     gene            complement(12251..13507)
                     /gene="icd"
                     /locus_tag="AC16QF_06465"
     CDS             complement(12251..13507)
                     /gene="icd"
                     /locus_tag="AC16QF_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIR6448233.1"
                     /translation="MTYQHIEVPADGEKITVNADDSLNIPDNPIIPFIEGDGIGVDIT
                     PVMKDVVDAAVAKAYGGSRRIHWMEIYSGEKSLEVYGEDEWLPAETLDAMREFIIGIK
                     GPMTTPVGGGIRSLNVALRQELDLYVCQRPVRWFQGVPSPVVAPEKTDMVIFRENTED
                     IYAGVEWEADSEGADKLIAFLRDEMGVNKIRFPQHCGIGIKPVSVEGSKRLIRKALQY
                     VIEEDRDSLTFVHKGNIMKFTEGAFKDWGYELAAEEFGATPLDGGPWHELTNPRTGKR
                     VIIKDVIADAMLQQILTRPDEYDVIATMNLNGDYLSDALAAQVGGIGIAPGANIGDTI
                     ALFEATHGTAPKYAGQDKVNPGSLVLSAEMMLRHMGWREAADLIIQGVEGAIGERTVT
                     YDFERLMSGATLLKCSEFGQAIVAKM"
     gene            complement(13633..14112)
                     /locus_tag="AC16QF_06470"
     CDS             complement(13633..14112)
                     /locus_tag="AC16QF_06470"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003316924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030366 - molybdopterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HIR6448234.1"
                     /translation="MHSEVRIQEADFSVAAQWEALRGRASGEVGAVASFVGLVRDRDP
                     DAPIQGLYLEHYPGMTEASIGKILDETASRWPILDIVVIHRVGGLEPGDQIVFVQVAA
                     AHRPAAFAACEFVMDYLKTDAVLWKRERLMEGERWIEATGDDRRRVARWKADPVGDS"
     gene            complement(14115..14372)
                     /locus_tag="AC16QF_06475"
     CDS             complement(14115..14372)
                     /locus_tag="AC16QF_06475"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR6448235.1"
                     /translation="MIPVRVLFFASLREAVGREEVFLELPDSARIDDLLASLDDILSA
                     EAMAALRAENVRLAVNQEMLTAPGRLRPNDEVAFLPPVTGG"
     gene            complement(14369..14845)
                     /gene="moaC"
                     /locus_tag="AC16QF_06480"
     CDS             complement(14369..14845)
                     /gene="moaC"
                     /locus_tag="AC16QF_06480"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019821998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR6448236.1"
                     /translation="MSKLTHLNAAGEASMVDVSGKSETLRRAVARGIVRMAPETLREI
                     LQSGVPKGDVIAVARVAGIQAAKRCADLIPLCHPLPLTKVALDFSPAGDDGLEVTAEC
                     VVTGQTGVEMEALTAVSVAALTVYDMCKAIDRGMVIESVCLVSKEGGRSGTWERTP"
     gene            14947..16035
                     /gene="mnmA"
                     /locus_tag="AC16QF_06485"
     CDS             14947..16035
                     /gene="mnmA"
                     /locus_tag="AC16QF_06485"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR6448237.1"
                     /translation="MATPRQTKVIVGMSGGVDSSVAALLLLQEGYAVEGLFMKNWEED
                     DGTEYCTAKEDLADAQRVAERLGITLHLANFAAEYWDNVFEHFLAEYDAGRTPNPDVL
                     CNREIKFKQFADYARALGADMIATGHYVRGAMQDGRFVLLKGVDENKDQSYFLQAVPA
                     EELARCLFPLGALTKTEVRAIAAQAGFENSGKKDSTGICFIGERRFRDFLQRYLPSRP
                     GPIKDTQAMTVGEHLGLAYYTLGQRQGLGIGGLAGRPEAPWYVVDKVTDGNTLVVSQS
                     EEDLLSEWLAAGETNWLAPEPELPRQCTAKVRYRQTDQPCLVSHRADGRLLVRFEQPQ
                     RAVTPGQYVCFYDGDVCLGGALIEATGS"
     gene            16032..16280
                     /locus_tag="AC16QF_06490"
     CDS             16032..16280
                     /locus_tag="AC16QF_06490"
                     /inference="COORDINATES: protein motif:HMM:NF016259.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF489 family protein"
                     /protein_id="HIR6448238.1"
                     /translation="MNAPEIDLARRLKGISRLGKRIQVADDPDLLKRESVADTIRAEL
                     LAGVRFAWLWQQLGGRRWHLVLRRRQLLVALETLERKT"
     gene            16289..17659
                     /gene="purB"
                     /locus_tag="AC16QF_06495"
     CDS             16289..17659
                     /gene="purB"
                     /locus_tag="AC16QF_06495"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009683124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR6448239.1"
                     /translation="MQTHPLMTIGALDGRYRNRLEPLAPLVSEFGLMRYRVLVECRWF
                     AHLAQTDSIGELPALSSGQAAALAAVAEDFSLADAERIKAIERTTNHDVKAVEYFVKE
                     RVAAIDGLASHIEFVHFACTSEDVNNLSYALMLRDSRELLGEQMQALIDQISAVADDA
                     AEIPMLSRTHGQAASPTTMGKELKNVAYRLSRQLRQLREVALLGKMNGAVGNYNAHVV
                     AYPEVDWPELARAFVEGLGLAQNPLTTQIEPHDYIAELFHALVRFNQILLDFDRDMWG
                     YIAIEYFVQQPVAGETGSSTMPHKVNPIDFENSEGNLGIANALLEHMAAKLPVSRWQR
                     DLSDSTVLRNVGSACGHCSVAYQASQRGLGRVKINADRIRADLDASWEVLAEAVQTVM
                     RKHGLIEPYEQLKAATRGKRIDRDLYREMLETLDLPASAREELRALTPASYTGLAADL
                     TKREPS"
     gene            17659..>18481
                     /locus_tag="AC16QF_06500"
     CDS             17659..>18481
                     /locus_tag="AC16QF_06500"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6448240.1"
                     /translation="MDIHVVPVDWSTHQHQLNEVRDAVFVREQGVPQELEWDGLDADA
                     THFLAVNEAGQALGCARLLGTGQIGRMAVVASQRRGGIGRRLLEAAVEKACEDGHAEV
                     FVHAQTEVARFYREAGFVPEGQEFMEADIPHIAMRLILPVEFRPSAEPIPAPLIVPQE
                     SDADDARAELKHLHGEPESIDALAACVAEPTRALLIYSQYLDHSLFDRPEIVDAVSRF
                     VRSAPPATLRILIHSSQPMVSRGHKLLELARRIDSKIEIRKVPDGLATDVHTYLVW"
BASE COUNT         3391 a         6165 c         5694 g         3231 t
ORIGIN      
        1 tcggcaaggg tctccgtctc ctctcccccg cgcccgaaga tgaacggatc cccgcccttc
       61 agcctgacca cgtgccgccc ggccagggca tgctcgacga ggagcgcgtt gatgccctcc
      121 tgccggatgc cgggaaactt gcgtttcttg ccgacgtaga tcttctccgc gtcacgccgg
      181 gcgtagtcga gcaggcgctg gttggccagg ttgtcgtaga gaatgacgtc ggccgcctgc
      241 agcaggcgca ggccctttaa ggtcaggagc tccggatcgc cggggcccgc ccccaccaga
      301 gcgaccgatc ctcggcgagc ggtttcggca gccgccggca ggagctcgtc gaacgccgcg
      361 gcagcctcct cgtcgcggcc ggtcagcacc tgctcggcca caggaccctg aatcagtgcc
      421 tcccagaatg cctggcgctc ggattcgctg ccgagcacgc tcttgacccg ctcgcgccgt
      481 tctttcacga agccggccag ctcgccaagc cgggccggca ggcgggcttc gagccagccg
      541 cgcacgacgc gggccagcgt gggggaactg ccgccggtgg aaacggctac cactacgggc
      601 gaacggtcca cgatgctcgg aaagatcgcg ctgcaaagct caggctggtc aacggtgtta
      661 accagtatcc gccgcgagac gcacatgtcg tagacgatgc ggttgacgtc cggatcgttg
      721 gtggccgcga ccaccagata gtgcccctcc acgtcgctcg gcctgaaccg acgcggaacg
      781 acgtccagac cgcgctcgtc caccagagcg cgggtcgcct cgctcaactc cggcgagacg
      841 atgaagatgg ctgcgccggc ccggatgagc agctcgatct tgcgcctggc cacctcgcca
      901 ccacccacca cgaggcaggg gcgctgcttg aggtcgagaa agagcggcag gtagtccacg
      961 gatctaagag ccggctctca accggcaccg gcagagaggt ccagcgattc ggcccccgcc
     1021 aggtagtcgc gcagagcatc gggtaccgcc accctgccct cgccatcctg gtaattctcc
     1081 aggatcgcga tgagcgtccg acccacggcg aggcccgagc cgttcaaggt gtgcacgagc
     1141 tcgggcttac cggactccgg atgccgatag cgcgccagca ttcgccgggc ctggaagtcg
     1201 aggaaattgc tgcacgacga gatctcccgg tagcagtcct gccccggcag ccagacctcc
     1261 aggtcgatgg tcttggccgc cgaaaagccg agatcaccgc cacagagcac cacgctgcgg
     1321 tagggcaatt ccagcttgcg cagaatcgtc tcggcgtggt ccgtcagctc ctccagcgcc
     1381 cgccaggaat caccaggccg aaccagcatg acgagctcca ccttctcgaa ctggtgctgt
     1441 cggatcatgc cgcgggtgtc tttgccgtag ctgcccgcct cgctgcgaaa gcagggcgta
     1501 tggcagacgt gacgaacggg cagctgatcg gctgccagga tcgtgtcccg gtagatgttg
     1561 gtgacgggca cctccgccgt gggcaccaga taaaactcgt tgctcccctc caggcgaaac
     1621 tgatcctcgg cgaacttcgg cagctggccc gtgcccatca acgactccgc gttgacgatg
     1681 tacggcacgt agacttcacg gtagccgtgc tcgatgacgt ggacgtccat catgaactga
     1741 accagcgccc gatgcagacg cgcgatcggc ccgtgcatga cgacgaagcg gctgccagtg
     1801 attttcgctg ccgtatcgaa atcaagcccg ccggcctctg tcagggccac gtgatcggcg
     1861 ggctcgaact cgaaggaccg ccgttcgccc cactgcccga tctcctggtt gtcgttctca
     1921 tcattgcccg ccggcacgga cgcgtccggc aggttgggaa tctcccgcag aaacccctga
     1981 taagtctcct ggagcgcatc gaagtcggct ttggccgcat ccaaccgatc gccgagatcg
     2041 ccgatctcgg ccagcagcgg ggcgatgtcc tcacccgccg ccttggcctt gccgatggcc
     2101 ttggatcggg tattgcgctc gttctgcaac gtctcggtca gctcctgcag acgctttcgt
     2161 tcgctttcga gggcttgcac gcgagagacg tccagaaggt aacccttgac ggcaagccga
     2221 tcgcgaacgc tttccggatc gtcccgtaat accttgatgt ctagcatggc gtcttcgcct
     2281 cttgccgata tgctcagtaa tagccgacca ggcgctggcc ggcccaggcc gcgacgatgc
     2341 agccgacaac gctcgcgaca acgtaaacaa cggcgccgcc ccagcgctgg ccctccacga
     2401 ggcccaccgt ttccagcgag aacgccgaga acgtggtaaa cccaccgagc aggccgacga
     2461 cgatgaggta gcgaccccac tgctcgaacc agggctgctg atgacctaaa ccccagagca
     2521 acccgatgcc gagcgagccc gcgatgttaa tggttagggt ggcccatggg aattgaccgg
     2581 gcacgaacag ccaggtgccg atcaggaacc gccccacggc gcccagcgcg ccgccgagag
     2641 caacccagac cagagcgttc gcggtcacct tcccgcccgg ccggacgtgt tttgatcgag
     2701 ccggcgcagg cggctcagcc gctcgcggat cttcgcttcc aggccctgat cggaaggctc
     2761 gtagaacgcc tggggatcca acccgtcggg ccagtaggtc tctccggctg cgtagtgctc
     2821 gggctcgtcg tgggcataac ggtagtcacg gccgtgaccc agatctttca tcagtcgggt
     2881 gggggcattg cgaaggtgca tgggcaccgc ttcagacgcc ccctcgcgca cgaagcccat
     2941 agcccgaccg taagcgacgt aagcggcgtt gctcttgggc acgctggcga gatacagcac
     3001 cgcctgggcg agcgccagct ccccctcagg cgagcccagg cgttcatagg tatcccacgc
     3061 tgcaagggcg atctgcaggg cccgagggtc cgcattgccg acgtcctccg tcgccatgcg
     3121 caccaccctg cgggcgacgt agagcggatc gcaaccaccg tccagcatgc ggcacagcca
     3181 gtagagcgcg gcgtccggag cactgccccg cacggatttg tgcagcgcgg agatctggtc
     3241 gtagaaggca tcgcccccct tgtcgaaccg ccggtagcgg ctgccggcgg cctccgtcac
     3301 cacagcctcg tccacctcct tctcggccag ctgagcggcc acctcgagca ggttcaacat
     3361 ccgccgcgcg tcgccgtcgg ccagcgaggt cagaagcgcg cgagcatcag gggtgaccgg
     3421 caggttcagc gcctgctccg ccgcccgatc gatgacccgg ttgagatctt cctcacccaa
     3481 aggcttgagc acgtaaaccc ttgcccggga caagagcgca gcattcacct cgaaagatgg
     3541 gttctccgtg gtcgccccga taaaggtcac ggtgcccttt tccacatggg gcagaaaggc
     3601 atcctgctgg gccttgttga accggtgcac ctcatcgacg aacagcaccg tggtctcgcc
     3661 caatccccgc aaggcttccg cctcagccac ggcggcccga acgtctttca ccccgctcag
     3721 cacggcagag aggctgatcc atcgagcacc ggcccgggcg gccagcagct tcgccagggt
     3781 ggtcttgccg gtacccggcg gcccccagag gagcatggaa tgaaggcggc cggactctgc
     3841 cagggtccgc aggggctttc cggcgcccag aagatgcgcc tgacccacga actcctccag
     3901 gcagttcggc ctgaggcgct cggctagcgg tgcgttctca tcccccaatg acatcggttc
     3961 ccggcggcac gacgagctga aactcgctcg attgtatcac cgggggttcg gccgacgtgc
     4021 tgaagaggat ctccgtgaac tgaccgagat gatccgtgat ggccagcgcg gccaaagcgt
     4081 tgtcggagaa atgcaggcga atctgcgcat acagggtgtc gggcgcgttg ggcgtgagga
     4141 tgtaggaagc accaagctcg tcggtgacct ccaccacctg aaactcgttg cctagggcta
     4201 cgccctcgcg ggtaagcagc gacaccggcg tgtccttcag cgcctccgcc agaggacgga
     4261 tcgtcagctg ctcgagatcc gcatcgtaaa ccttcagccg atctccgtcg gtcaccaccg
     4321 tctgcgggta agggtggtcg acttcccact tgaacagcgg ccggtcgaga acgaaccgtc
     4381 cggtcgagcg ctccagcacc tggccctgag agccgcgaat ctcctggctg aactccgcca
     4441 cgaacacgtc cattgcggcc agccgttcgg atagcgcctg ccgggggtcg gccgccgccg
     4501 aggcgcagga tagcgcggcg acgagccaga cgacggggtc agtcgcgatt cggaaccagg
     4561 acttcacgac tgccgttgct gttcatggcg cttaccacgc cggctgcttc catggcttct
     4621 atgagccgcg cggcacggtt gtagccgatc cggagcttgc gctgcacgga tgagatggac
     4681 gcgcgccgcg actcgaggac gaaggccacc gcctcgtcgt acagggcgtc ctcctgctcc
     4741 gctgacccgg aatccggctc gatggagaga aaaggctcgt cgtcaccgcc ggacagcacg
     4801 ccgttgtcgt aatcgggttc gccccgctgt ttccagtcgg agactacccg gtgcacctcc
     4861 tcgtcggaga cgaacgcacc gtgcacacga gtcggcagtc cagagcccgg tggcaggtag
     4921 agcatgtccc catgacccag aagctgctcg gccccgccct gatcgaggat cgtgcgcgag
     4981 tccaccttgg acgacacctg aaagctgatg cgcgaaggaa tgttggcctt gatgagaccc
     5041 gtaatcacgt ccaccgacgg acgctgcgtc gccaggacca gatggatccc ggccgctcgc
     5101 gccttctggg cgatgcgggc gatcagctgc tccaccttct tgccgacgat catcatcatg
     5161 tcggcgaact cgtcgatgaa cacgacgatg agcggcagct tgccaagggc cgccggctcg
     5221 ccttccgaat cggcgtccca cagcggatcc gagatcgtct gacccttctt ggcggcgtct
     5281 gcaatctgac ggttgtagcc ggccaggttg cgcacgccga gcgccgccat gagcttgtag
     5341 cgtcgttcca tctcggccac gcaccagttg agcgcgtgcg ccgcctcctt catgtccgtc
     5401 accaccggcg tgaggaggtg aggaatcccc tcgtacaccg acagctccag catcttggga
     5461 tccaccagga tcatccggac gtcttccggg gtggccttgc agaggatgct cagcagcatg
     5521 gcgttgaccc cgacggactt acccgaaccc gtggtgccgg ccaccagcag gtgaggcatg
     5581 cgggagatgt cggccaccac ggactcgccg gcgatgtcct tgcccagggc cagggtgagc
     5641 ggcgatttcg agtcctgaaa gacggccgag ctgaaaacct ccttcaaccg caccacctcg
     5701 cggtgctcgt tggggatctc gatgcccacc acggatttgc cgggaatcac ctccacgatc
     5761 cgcacgctga tcaccgccag ggaacgggcc aggtccttca ccagggagct gattttctgg
     5821 accttgaccc ccgcagccgg ctgtatctcg aaccgcgtca ccacggggcc gggcagcacg
     5881 gagaccacct cggcctctac gccgaagtcc ttgagcttga gctcgagcag tcgcgagatc
     5941 gcctcgaggg attcatccga gtacccgcga tcgtccccgg gctgacgctc gtcgaaaagg
     6001 tccaatcccg gcagatcgcc gctgggcgtg gattcgaaaa gcggttcctg cacggcaacc
     6061 gtcgacgctt tctgcgcggc ggcgggtttg atggtcggct tcgcgcggct cccgcgcttc
     6121 gcgcgctcgt cgcccagggc ttcctcccgg gcacgcatca tctccttagc gcccgagcgg
     6181 gcttcgcgaa aacgacgcca gcggtccagc tgtcgatcga gccagagcac ggcgtttgcg
     6241 gtaaaggcgt gcacccacct tcccgtcagc tccgcgacgc tcagccagga gaaaccggcg
     6301 gccgcctggg cgccgatcat caccgcggtc agcgcgatga gcgtaagccc gatccagccg
     6361 aacacgggca gcccgagggc gaccagggct tcccccaata tgccacccgc accgccaggc
     6421 aaggccgccg ccggagcaaa gtgaatgcgc gcgaggatgc agccgaagat cagaaccgcc
     6481 agccagccgg cgcagcgcag ggcgagcagc caccacgaaa acgtttcgct gcgttccctg
     6541 aagagccgaa cgccaatgac caccagcgca acgggcagga tgtacgcaac ccttccgaac
     6601 agatacaagg tgaagtcggc gaaccaggcg ccactgcgac ccaccagatt gtcgacgctc
     6661 ccgccggtgc ccgtgaacga gaacgcggga tcagcgggtg agtaggaaac catggcgagc
     6721 atggtgtaca gggcggcggc cgtgaatcca atcaggccga tttcccggac aggaatcgtc
     6781 gatgtggtca cgaatttctc cttaaattcg gcgcgaagtg tagcggcaaa accatctgcg
     6841 aacaaatctc tatttaattc aattcgttac ccgaactacc tcaatcagaa cttgccgact
     6901 taccggcagt ccaaccaatc gttatcatag ccaccgaacc tgaacacggg ccaaacatgc
     6961 gaccagacaa aacgaaagtc gtcgacgagg tgtgggatga cgaccgcatc gaagggttcc
     7021 tgcacaaagc gccgctgggc gatgagcgca gcgccgacta ctcagcgctg ctgtacgcct
     7081 accgcagcat gcggcccgac gatttcgcca cgttcattcg cttgttcgtt gacgccggac
     7141 gggacgtcaa cgcgaaaagc aacggcggag aaaccctgct tgccaccatc cgcgaccacc
     7201 gccatggcgc gcctttccgc gccattctgg aaaaggccgg cggtagcgcg agctgaagcg
     7261 aagcgcctag aacgatgtcc gggctaagcg tgctatcaag ccaccgtgtg gggtttcccc
     7321 caccagagtc cgccctcgcc gaacccaacg gcctgctggc cgtgggcggc agcctgacgc
     7381 cggaatggct gctggcagct tacgcgcgcg gcatctttcc ctggttcgac gacgatgcat
     7441 caccccttct ctggtggagc cccgaccccc gggccgtgct cttgccggag gaactgcggg
     7501 taacgcgcag ccttgccaaa cgaattcgca atgcaggttt caatgtgacc atggaccagg
     7561 cgtttgcgga tgtggtcgca gcatgcgccg cgccccgcgg cgcggaggcc ggcacctgga
     7621 tcaccgcgga catggcacgc gcctacgccg aacttcacga gatgggcttt gcccactcgg
     7681 tggagacccg gctggacggc gagctggtgg gggggctcta cggcgtttct ctggggcgca
     7741 tgtttttcgg agagtccatg tttgccaggc agcgggacgc ttcgaaggtc gccctgtacc
     7801 atctgaccct gcgcgcccgg gactggaatt tctctctcat cgactgtcag atcatgaacg
     7861 agcacctgcg ctccctaggc gcccgggaaa tgccccgagc cgagttcctc cggctagtcc
     7921 atgaaaaccg ggccgagcct acgcgccgcg gatcgtgggc ggacgagacg gcccgatgaa
     7981 agacgagacc gccgacaagc agttcttcat cacgcccgcc cacccctgca gctacctgcc
     8041 taagcgcaac gcccagacgc tctttctcga tccccgggag acgatcacgc cgcaaaccta
     8101 ccagcagctc accgagcagg gttttcggcg cagtggcggc catctttacc ggcctcactg
     8161 caagacctgc caggcctgcg tgcccacgcg cctccccgtc gccgagttcg ttgcgcgccg
     8221 gagccagagg cgaaccctgg cacgcaatac ggacctggcc gttcgggtgg aacccgccgc
     8281 gtacagcccc gcccattact cgctttacgc cagctacatc gcaggccgcc atggggatgg
     8341 cgacatgtac ccaccctccc ccgaccagtt ccgttctttt ctcttgagtc agtggtcgga
     8401 caccctgttc gtcaacagct tcttggatgg caagctcgtt gccgtggcgg tcacggacaa
     8461 gctggatgat gggctttctg ccatctacac tttcttcgat ccggcgctag agcagcgcag
     8521 cctgggtgtg cagagcatcc tccagcagat cgagctcgcc cgggaactcg ggctgtccta
     8581 tctgtatctg ggctactgga tccgcgacgc ggaaaaaatg cgctacaaga ccgactaccg
     8641 ccccaccgag ctgttcgtga acggacggtg ggtgcgtatc acctgagctt tgcatgtgcc
     8701 cggaccatga ggcagaatgc cgcacgcaaa attcgggccg cccgtaaacg gacggcgtca
     8761 ggagtatatg gctaaagaag aacagatcga gatggaggga acggtgatcg acaccctccc
     8821 caacaccatg ttccgagttg agctggacaa cgggcacgtg gtcaccgcgc acatctccgg
     8881 caagatgcgc aaaaattaca ttcgcatcct caccggcgac aaggtcaagg tagagcttac
     8941 cccctacgac ctcagcaaag gccgcatcac cttccgcaaa taaacttcaa gtggtcgccg
     9001 ccggcgtctt gtcggcggac ccttcgggcg cttcgcagcg caggtgcagg tcgccgtctt
     9061 tgaccgtgac gtaaacggtc ccgccattct tcgacagcgc accgaacagc acgtcttcgg
     9121 ccaactggcg cttgatgcgc tcctgaatca accgctgcat gggccgggcg cccatcttct
     9181 cgtcgtagcc ctcgacgacg agccactccc tcgcagcatc gtccacctcc agggtgactt
     9241 tcttgtcgtc cagctgggcc tggagctcga tgaggaactt gtccaccacc gtcttgatga
     9301 cctctttggg cagggagtgg aactgaatga tgccgtcgag ccgattgcgg aattccggcg
     9361 tgaacagctt cttgatggcc tccatcgcgt ccgaggtgtg gtcctgctga gtgaagccga
     9421 tggagggccg gctcatgtcc tgggcccccg cgttggtggt catgaccagc accacgttgc
     9481 ggaaatcggc cttccggccg ttgttgtcgg tgagcgttcc gtggtccatg acctgcagca
     9541 gcaggttgaa gacttccgga tgggccttct cgatctcgtc cagcagcacg atgctgtggg
     9601 gatgcttggt aaccgcctcg gtaagcaggc cgccctggtc gaagcccacg tatcccggcg
     9661 gcgccccgat gagtctggag accgtgtgcc gctccatgta ctccgacatg tcgtagcgca
     9721 gcagctccac tcccatgatg tttgccagct ggcggcagac ctccgtcttg cccacgcccg
     9781 tagggccggc aaacaggaac gagccgatgg gtttctcacc ggacttcaag cccgcgcggg
     9841 agagcttgat ggccgaagcg agcgaatcga tggcctcgtc ctgaccgaac accaccatct
     9901 tcagcttggc atccaggttg cgcagcgcct ccttgtccga gctggtcacc tgggtcgacg
     9961 ggatccgcgc gatcttcgcc acgacctgct ccacgtcggt cgctccgatg ctcttcttgc
    10021 gcttgctggg agcgagcagc agctgggctg cgccggcctc gtcgatgacg tcgatggctt
    10081 tgtccggctg gaagcggtcg gtgatgtacc ggtctgcaag ctcggcagcc gcacgcagcg
    10141 ccttgtccgt atagcggatg tggtagtggt cctcgaaccg tgatttcagc cccttgagaa
    10201 tgttgtaggt gtcgttgacg tccggctcgg tgatgtcgat tttctggaaa cgccgggaca
    10261 gcgcccgatc cttgtcgaaa atcccgcgat actcctgata ggtagtggac cccatgcagc
    10321 ggatgtcgcc ggaagtcaga agcggtttga gcaggttcga cgcgtccatc acgccgccgc
    10381 tggcagcacc ggcgccgatg atcgtgtgca cctcgtcgat gaagaggatg ctgccttcct
    10441 gctttttcag ttcggccaga acgagcttga aacgcttttc gaaatcgccg cgatacttgg
    10501 tgcctgccag cagcgcgccc atgtcgagcg cgtagatggt gctgtcggag accacctcgg
    10561 gcacctggcc gtccacgatg cgcttggcca aaccctcggc gatggccgtc ttccccacgc
    10621 ccgactcgcc gacgagcagc gggttgttct tgcgccgccg gcaaagcacc tgaatgaccc
    10681 gctccagctc agcatcccgg cccacgagcg gatcgatgcg cccgttgcgt gcctgatcgt
    10741 tgaggttcgt tgcgaaagct tccagggcgc tctgctgggc ctcttcgccg gcgccttcct
    10801 cttcctgctc ggccgcgggc tccccggatt ctccttcgcc gaagaccttg gagatgccat
    10861 gggcgatgta gttaacgacg tcgatgcgcg cgacgttctg ctgcttcaag aagaacaccg
    10921 cctggctttc ctgctcgctg aatatcgcaa cgaggacgtt cccgccggtg acctccttgc
    10981 gccccgacga ctggacgtgg aacacggcgc gctgcagaac ccgctggaag ccgagcgtcg
    11041 gctgggtctc ccggtcggag tctccggccg gaatgagcgg cgtggtgctc tcgatgtact
    11101 ccgaaaggtc cttgcgcaac cgatcgacgt ccacgcccac cttctcgagc acgtccaagg
    11161 cgaccttgtt gtccagcagc gccagcagca ggtgctcgac ggtcatgaac tcgtgacgct
    11221 tggctttggc agcgcggaac gcttcgttca gggtaacttc taaatcttta ctcagcatga
    11281 ggtgtgtctg gcttcgtcct gtgttcggtg atcaatcggt catctctatc gtcgagatca
    11341 gcggatgctg attctcctga gcatattgat tcacttgctc ggatttagtt tcagcaatgt
    11401 cgcggggata gatccccacc accgccgcgc cctgggtgtg caccgtgagc atgatctgcg
    11461 tcgctttctc gcgatccatg gcaaaaaaga tctccagcac gtgcacgacg aactccatgg
    11521 gcgtgtaatc gtcgttcagc agcaccacct tgtacatcgg cgggcgcttg agttccggct
    11581 tggcggtgac cgtcgcgagc cccgaatcgg agctgtcgtc cgcgtcaccc tcgccggatt
    11641 gcgcttgcaa cgggtcgctc ttcagaaccc tgggcggtat ctgggaacga tttgttggct
    11701 ccattgctcc tcgttcatgt gggcggcagc acgatgatac aagcatcccc gggccttgac
    11761 taagcccgaa aatccatgga aagtcgggct cataggggag ctgcaacttg tgaccgttcg
    11821 attatacgcg gcgggacggg tacaaagatg cgcaacactg cgcgcttcgc gcagtttggg
    11881 cggcagggcc gaggtacgca caaggatggg cgttgtgttc gtggctgcta gggggtctcc
    11941 atggcgatgg gaaaagtaaa gtggtttaac aatgccaagg ggtacggatt tatcctgccc
    12001 gacggcggtg gcgacgatct gtttgcgcac tactcttcca ttcagatgga cggctacaag
    12061 actctcaagg ccggccagga cgtggagttc gacattctag agggccctaa aggcctgcac
    12121 gccatcaaca tacgcgcgga ggcgattgaa tccgaggatt caggcgagga cgcccccgtc
    12181 gacgagagcg cggcagctga tcccggaccg gcagaggcct ctccaccggc ccgagaaacc
    12241 tctcgcgagg ctacatctta gcaacgatgg cctgaccgaa ttccgagcac ttcaacaacg
    12301 tcgcgccgct catcaggcgc tcgaaatcgt aggtcaccgt ccgctcgcca atggcgcctt
    12361 ccacaccctg aatgatcagg tcggcggcct cgcgccaccc catgtggcgg agcatcatct
    12421 ctgccgagag caccagcgag ccgggattca ccttgtcctg ccccgcgtac ttcggcgcgg
    12481 tgccgtgggt cgcttcgaac agcgcgatgg tgtcgccgat gttggcacca ggcgcgatac
    12541 cgataccgcc gacctgagcg gccagggcat cggacaggta gtcgccgttc agattcatgg
    12601 tggcgatcac gtcgtactcg tctggccggg tgaggatctg ctgcagcatg gcatcggcga
    12661 tcacgtcttt gatgatgacc cgcttgcccg tgcgggggtt ggtcagctca tgccaggggc
    12721 cgccgtccag cggggtcgcg ccgaactcct ccgcggctag ctcgtagccc caatccttga
    12781 aggcgccctc ggtgaacttc atgatgttgc ccttgtgcac gaaggtcagc gagtcccggt
    12841 cctcctcgat gacgtactga agggctttgc ggatcaggcg cttgctgccc tcgacggaaa
    12901 cgggcttgat gcctatgccg cagtgctgag gaaagcggat cttgttgacc cccatctcgt
    12961 cgcgcaggaa agcgatgagc ttatcggccc cttccgaatc cgcctcccat tccacgcccg
    13021 cgtagatgtc ctcggtgttt tctctgaaga tgaccatgtc cgtcttctct ggggccacca
    13081 ccgggctggg gacaccctgg aaccagcgca ccggacgctg acagacatac aggtccagct
    13141 cctgtcgcag agcgacgttc aaagaccgga tgccgccgcc caccggcgtg gtcatggggc
    13201 ccttgatgcc gatgatgaac tcccgcatgg cgtcgagggt ctccgccggc agccactcgt
    13261 cttccccgta gacctccagg gatttctcgc cggagtagat ctccatccag tggatcctgc
    13321 ggctgccgcc gtaggccttg gccaccgcgg cgtccaccac gtccttcatg accggggtga
    13381 tgtcgacccc gatgccgtcc ccctcgatga aagggatgat cgggttgtcg gggatgttca
    13441 gtgaatcatc tgcgttgacg gtgatcttct cgccgtcagc gggcacttcg atgtgctgat
    13501 aggtcatcta aagctccgtt tggaaatccg tttgggtaac agccttgacc cgtcgcgata
    13561 ggtcgcgatg gaaaggcgca ccggaaaaag gcgcgagatt atagcccttc cgacgaggct
    13621 gtgcagtggg tatcaggaat cgccgaccgg gtcggccttc catcgggcga cacggcgacg
    13681 gtcgtccccg gtcgcctcga tccagcgctc gccttccatc agccgctccc gcttccacag
    13741 cacggcgtcg gttttcagat agtccatgac gaactcgcag gcagcgaagg ctgccgggcg
    13801 gtgcgcggcc gccacctgca cgaacacgat ctggtcgccg ggttcgaggc cacccacgcg
    13861 gtgaatgacc acgatgtcca ggatgggcca gcgtgatgcg gtctcgtcga gaatcttacc
    13921 gatgctcgct tccgtcatgc cgggataatg ctcgagatac agaccctgga tgggcgcatc
    13981 aggatcgcgg tccctcacga gcccgacaaa gctcgctacc gcgccaacct cgcccgacgc
    14041 gcgcccgcgc agcgcctccc actgtgccgc cacgctgaaa tccgcttcct gaattcgaac
    14101 ctcggaatgc acggtcagcc ccccgttacc ggcgggagga aggcgacctc atcgttggga
    14161 cgaagccgcc caggggccgt gagcatctcc tggttgacgg ccaggcgaac gttttccgcc
    14221 cgcagcgctg ccatcgcctc ggcgctgaga atgtcatcca gcgaggcgag caggtcgtcg
    14281 atccgcgccg aatccggcag ctcgaggaag acctcctcgc gaccgacggc ttccctgaga
    14341 gaagcgaaaa acagaacacg gacgggaatc acggcgtgcg ttcccaggtg ccgctgcggc
    14401 cgccttcttt gctcaccagg cacacggact cgatgaccat gccccggtcg atggctttgc
    14461 acatgtcata gaccgtcagc gcagccacgc tgacggcggt cagggcttcc atctcgaccc
    14521 cggtctggcc cgtgaccacg cactcggcgg taacctcgag accgtcgtcg ccggccgggc
    14581 tgaaatccag agccaccttg gtgagcggca acggatggca aagcgggatg agatccgcgc
    14641 accgtttggc ggcctgaatg ccggctacgc gggcgaccgc aatgacgtca cccttgggaa
    14701 cccccgactg caggatctcg cgaagggtct ccggcgccat ccgcacgatc ccccgggcca
    14761 cggcacgccg cagcgtctcg gacttgccgg agacgtcgac catgctcgcc tcgcccgcgg
    14821 cattgagatg ggtaagcttg ctcatggtcg cattatagcc cccaacgggc tgtcgggcgg
    14881 gcaagaaccc ctgcttgggg gtagaatggc catccttcac actgcagtgc tgcacgcacc
    14941 cctgaagtgg caactcccag gcaaaccaaa gtcatcgtcg gcatgtcggg cggtgtcgac
    15001 tcctccgtcg ccgccctcct gctcctgcag gaggggtacg ccgtggaggg cctgttcatg
    15061 aagaactggg aagaggacga cggcaccgag tactgcacgg ccaaagagga tctggccgac
    15121 gcccaacggg tcgccgaacg cctgggcata actctgcacc tggcgaactt cgctgccgag
    15181 tactgggaca acgtcttcga gcactttctc gccgagtacg acgcgggccg cacgccgaat
    15241 cccgacgtgc tctgcaaccg cgagatcaaa ttcaagcagt tcgcagacta cgcccgcgcc
    15301 ctcggcgcgg acatgatcgc cacggggcac tacgtgcgtg gcgccatgca ggacggccgc
    15361 ttcgtcctgc tcaagggcgt ggatgaaaac aaggaccaga gctacttctt acaggccgtc
    15421 ccggctgagg agctcgccag gtgcctcttt ccccttggcg cgctgaccaa aaccgaggta
    15481 cgtgccattg ccgctcaggc aggattcgaa aactcgggca agaaggacag cacgggcatc
    15541 tgcttcatcg gtgagcgacg cttccgggac ttcctgcaac gctatctccc aagccggccg
    15601 ggccccatca aggacaccca ggcaatgacc gtgggcgagc acctgggcct cgcctactac
    15661 accctgggcc agcgtcaggg gctgggcatc ggcggcctgg caggacgtcc cgaagcgccg
    15721 tggtacgtgg tggacaaggt cacggacggc aacacgctcg tagtgtctca gagcgaagag
    15781 gacctgctca gcgagtggct ggcggccggc gagacgaact ggctggcacc ggagcccgag
    15841 cttcccaggc aatgcacggc gaaggtccgc taccgtcaga cggaccaacc ctgcctggtc
    15901 agccaccgag ccgacggtcg ccttctggtg cgcttcgagc aaccccagcg ggcagttacc
    15961 ccgggccagt acgtgtgttt ctacgacggc gacgtttgcc tgggcggcgc gctgatcgag
    16021 gcgacgggct catgaacgcg cccgagatcg acctggcccg aaggctaaag ggcatcagcc
    16081 ggctcggcaa gcgcatccag gtcgccgacg atcccgatct gctcaagcgt gaaagcgtgg
    16141 ccgatacgat ccgcgccgag ctgcttgccg gcgtccggtt cgcctggctg tggcagcagc
    16201 tgggcggccg ccggtggcat ctggttttgc gacgccggca gctgctggta gccttggaaa
    16261 ctctcgagag gaaaacgtga ccacctccgt gcagacacac ccgctcatga ccatcggcgc
    16321 cctggacggg cgctaccgga accgcctcga accactggca ccgctggtaa gcgagttcgg
    16381 actgatgcgc taccgcgtgc tggtggagtg ccgctggttc gctcatctcg cccagaccga
    16441 ttcaatcggc gagctgcccg ccctatcttc cggccaggcc gctgcccttg ccgccgtggc
    16501 ggaagacttt tcgctcgcgg acgccgaacg catcaaggcg atcgagcgga ccacgaatca
    16561 cgacgtgaag gccgtggagt acttcgtcaa ggagcgcgtc gcggctatcg acgggctcgc
    16621 ctcgcacata gagttcgtcc acttcgcctg cacgtcggag gacgtcaaca atctctctta
    16681 cgccctgatg ctgcgcgact ctcgcgagct gctcggcgag cagatgcagg cgctcatcga
    16741 tcagatcagc gcggtcgccg acgacgcggc cgagatcccg atgctgtccc gaacccacgg
    16801 tcaggcggcc tcgcccacca ccatgggcaa ggagctcaaa aacgtcgctt accgcctgtc
    16861 ccgtcagctc aggcagctgc gtgaggtggc cctgctgggc aagatgaacg gggcggtcgg
    16921 caactacaac gctcacgtgg tggcctatcc cgaagtcgac tggcccgagc tggcgagggc
    16981 tttcgttgaa ggcctgggcc tcgcgcagaa cccgctgact acccagatcg aaccccacga
    17041 ctacatcgcc gagctctttc acgcgctggt cagattcaat cagatactgc tcgacttcga
    17101 ccgcgacatg tggggctaca tcgccatcga gtacttcgtc cagcagccgg tcgccggcga
    17161 aaccggctcg tccaccatgc cacacaaggt caaccccatc gacttcgaga actccgaggg
    17221 caacctgggc atcgccaacg ccctgctcga gcacatggcc gccaagctgc ccgtctcgcg
    17281 ctggcaacgg gacctgtcgg attcaaccgt gcttcgcaac gtcggttctg cctgcggcca
    17341 ctgcagcgtc gcctaccagg cgagccagcg cggcctcggc cgcgtgaaga taaacgctga
    17401 ccggatccgg gccgacctgg acgccagctg ggaagttctg gcggaggccg tacagaccgt
    17461 catgcgcaag cacggcctga tcgagcccta cgagcagctg aaagccgcca cccgcggcaa
    17521 gcggatcgat cgcgatctgt acagagagat gctcgagacc ctcgatctgc ccgcgagcgc
    17581 cagggaagag ctgcgcgcgc tgacgccggc ttcctacacc ggcctggccg ccgacctcac
    17641 caagagagaa cccagctgat ggacattcac gtcgtgcccg tggattggtc gacccaccag
    17701 caccagctca acgaggtccg cgacgccgtc ttcgtcaggg aacagggggt gccccaggaa
    17761 ctggagtggg acggtctcga tgcagacgcc acgcactttc tcgccgtcaa cgaagcgggc
    17821 caggcgctgg gctgcgccag gctcctcggt accggccaga tcgggcgaat ggcggtggtg
    17881 gcgtcccagc gtcgcggcgg gatcggccga aggctcctgg aggcggccgt ggagaaagcg
    17941 tgcgaggacg gccacgccga ggtcttcgtt cacgcccaga ccgaggttgc ccgcttctac
    18001 cgcgaagccg ggttcgtgcc cgaagggcag gaattcatgg aagcggacat accgcacatc
    18061 gccatgcgtt tgatcctgcc cgtggagttc aggccaagcg ccgagccgat tcccgctccc
    18121 ctgatcgtcc cccaggagag cgacgcagac gatgcgaggg ccgagctcaa gcacctgcat
    18181 ggcgagccgg agagcatcga tgccctcgcc gcgtgcgtgg ccgagccgac cagggcgctg
    18241 ctgatctaca gccagtatct ggaccactcg ctgttcgacc ggcccgagat cgtcgatgcg
    18301 gtctcgaggt tcgttcgcag cgcgccgcct gccacgttga gaatcctcat ccattccagc
    18361 cagcccatgg tgagccgcgg ccacaagctc ctggagctcg cccgacggat cgacagcaag
    18421 attgaaatcc gtaaggtgcc agacgggctc gccacggacg ttcacaccta tctggtgtgg
    18481 g
//
LOCUS       DBNHLM010000137         3242 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_79367, whole genome shotgun sequence.
ACCESSION   DBNHLM010000137 DBNHLM010000000
VERSION     DBNHLM010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_79367"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="AC16QF_06505"
     CDS             <1..425
                     /locus_tag="AC16QF_06505"
                     /inference="COORDINATES: protein motif:HMM:NF019473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HIR6448241.1"
                     /translation="LKAMEDGDLAAKIEELCTDDFVWANSGLPTLSGKTAVRAHMEAG
                     GFSRQIPILAAMTSFSADVLHIASRAGVVFTERVDHHWDADGRDLMTPHIAGVMEIRD
                     GRIAALRDFYDVACYAQTPTAPNPDHAMAGTNDKGELT"
     gene            422..967
                     /locus_tag="AC16QF_06510"
     CDS             422..967
                     /locus_tag="AC16QF_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6448242.1"
                     /translation="MKRTVDAEHLIKPAKFAHFVLRVSDIEASIDWYATVLGMEMVHR
                     ADMLAFMTYDEEHHRLALVQTPVEAATPGAPGLDHVAYTLGSLGDLMATYKRLKAADI
                     LPVWPINHGLTTSLYYADPDGNRVEFQVENLDSVEDLQGFMRSDAFAANPIGVGFDPE
                     KLLQRYENGDPLEELLQQGSA"
     gene            complement(1031..2380)
                     /locus_tag="AC16QF_06515"
     CDS             complement(1031..2380)
                     /locus_tag="AC16QF_06515"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR6448243.1"
                     /translation="MAGSSREQWSSRFGFLMASIGFAVGLGNIWRFPYITGENGGGAF
                     VIVYLLCAFGIGVPILMAEVMIGRRGRLSPPGSMREVAVAEGRSRQWQWAGGLNLATA
                     FIIEVIYCVVAGWVLYYLYQAVTGGFVGVDAGSAQAGFAQLMDDTGTMLFWTVVGLVV
                     TGLIIYAGVEKGIERAVRILMPTLFGLLVLLAVYNVFAGGFGQALDYLLSADFSKLNA
                     ATVLIAVGQAFFSIGVALAGMMTFGAYLPRDVSIGQSVLVIVFADTLVALTAGLVVFP
                     AVFHFGLDPAGGPGLIFQTLPVAFGQMPGGRLVSILFFLLLSVAAVTSMVGLLEPLTS
                     WLEEHRGLSRHGSAVLVLGSVAALSVLSILSYNVLTGFRVFGRDLNGVFDYLSNQIML
                     PLGGFLIALFVGWFVSKATSRDELDIPDSRYFQTWHALIRYVVPPAVLAILVFGVMS"
     gene            2551..3141
                     /locus_tag="AC16QF_06520"
     CDS             2551..3141
                     /locus_tag="AC16QF_06520"
                     /inference="COORDINATES: protein motif:HMM:NF016269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HIR6448244.1"
                     /translation="MFRTHRTATLVTAILMMLISGSAAADKYDETVELFRNAGDSNEF
                     FAKSYGYAVFPTIGKGGFGIGGARGKGRVYVGGNHVGNTTMTQLTIGFQIGGQGFSQI
                     IFFQDERSFREFSTGNFEFGANASAVAITAGASASASTSGATAGASGGKKDATTAGTA
                     YYKGMKVFTIAKGGLMYEASIGGQKFSYRPAGGDQS"
BASE COUNT          683 a         1086 c          995 g          478 t
ORIGIN      
        1 tgctcaaggc gatggaggat ggtgaccttg ccgccaagat cgaggagctg tgcaccgacg
       61 acttcgtctg ggccaacagc gggctgccga cgctgagcgg caagaccgcc gtgcgcgctc
      121 acatggaagc cggtggcttt tcccggcaga tacccatcct ggctgctatg acgtctttca
      181 gcgccgacgt gctgcacatt gccagccggg ccggggtggt gttcaccgag cgcgtcgatc
      241 accattggga cgccgacggc cgggacctga tgacaccgca catcgcgggc gtcatggaaa
      301 tccgcgacgg caggatcgcc gccctgcgtg acttctacga cgttgcctgc tacgcgcaga
      361 cgcccacggc cccgaatccc gaccatgcca tggcaggcac caacgacaaa ggagagctga
      421 catgaagaga accgtcgacg cagagcatct gatcaagccg gccaagttcg cgcacttcgt
      481 gctgcgcgta tcggacatcg aagcatccat cgactggtac gccaccgtgc tcggcatgga
      541 gatggtgcac cgggccgaca tgctggcgtt catgacctac gacgaggagc atcaccgcct
      601 ggccctcgtg caaacgcccg tcgaggctgc cacccccggt gctccgggac tcgaccacgt
      661 agcctacacg ctgggctccc tgggcgatct catggctacc tacaagcgct tgaaggcagc
      721 ggacatcctg ccggtgtggc cgatcaacca cgggctgacg acctccctgt actacgcgga
      781 ccccgacggc aaccgagtcg agttccaggt cgagaacctc gactccgtgg aggacctgca
      841 gggcttcatg cgttcagatg cctttgccgc gaaccccatc ggcgtgggct tcgaccccga
      901 gaagttgctg cagcgttacg aaaacggcga tccgctcgag gaactcctcc agcagggctc
      961 ggcctgacgc aggaacgcgc cgggccaaca gggtcttcga tcgacccacg ccgcaggccg
     1021 gcgcgcgtcg tcagctcatc acgccaaaaa cgagaattgc cagcaccgcc ggaggcacca
     1081 cgtagcgaat caacgcgtgc cacgtctgga agtaccggga gtcgggaatg tccagctcgt
     1141 cccgcgatgt cgccttgctg acaaaccagc caacgaacag cgcgatgaga aagccgccga
     1201 gcggaagcat gatctggttc gacaggtagt cgaacacgcc gttgaggtcc cgaccgaaaa
     1261 cgcggaatcc ggtgaggacg ttgtaggaga gaatgctcag cacgctcagg gccgccaccg
     1321 aacccagcac gagcaccgcg ctgccgtgcc ggctgaggcc ccggtgctcc tcgagccagg
     1381 acgtgagcgg ctccaagagc cctaccatgg aggtaacggc cgcaacggag agcagcagaa
     1441 agaagagaat gctgaccagc cgcccacccg gcatctggcc gaaagccacc ggcagcgtct
     1501 gaaagatgag gccggggccg ccggccgggt cgagaccgaa gtgaaacacc gcgggaaaga
     1561 ccaccagccc cgccgtcagc gccacgaggg tgtcggcgaa cacgatcacc aggacgcttt
     1621 gaccgatgga aacgtcgcgc ggcaggtagg cgccgaacgt catcattccc gccagcgcta
     1681 cgccgatgga gaagaaggcc tggcccacag ctatcaggac cgtggccgcg ttcagcttgc
     1741 tgaagtccgc cgagagcagg taatcgaggg cctggccgaa accgccggca aacacgttgt
     1801 agaccgcgag cagaacgagg aggccgaaca gcgtcggcat gagaatccgc accgcgcgct
     1861 cgatgccctt ctccacgccg gcatagatga tgaggccggt aacgaccagg ccgactaccg
     1921 tccagaacag catggtgccg gtgtcgtcca tgagctgtgc aaagccggcc tgtgccgagc
     1981 ccgcatcgac accgacgaac ccgccggtga cggcctgata aagatagtag agaacccagc
     2041 ccgcaacgac gcagtaaatc acctcgatga tgaaggcggt ggcgagattc agcccgcccg
     2101 cccactgcca ctgacgggag cgaccctccg ccacggcgac ctctcgcatg gatcccggcg
     2161 ggctgagccg cccccggcgc ccgatcatca cctcggccat cagtatcggc acgccgatgc
     2221 cgaaagcgca gagcagatag acgatcacga acgcgccgcc gccgttctcg cccgtgatgt
     2281 acggaaagcg ccagatgtta ccgagaccca cggcaaagcc gatggaggcc atcagaaacc
     2341 cgaagcgcga cgaccattgc tcccggctcg atccggccat ggcacagctc tcgtcaagta
     2401 gacgggctaa tctaacctcg aagcccccct tgatgcacgg ccgaggtcgg ttcaggcgcg
     2461 gttcagttgc agggattatc ctttcgaaca agctgggaag tgctatcctt ccggccgctc
     2521 gagaactgct ttcttcaagg agtcgtctga atgtttcgaa ctcatcgaac cgccaccctg
     2581 gtcaccgcca tcctgatgat gctcatctca ggcagcgctg cagcggacaa gtacgatgaa
     2641 accgtggagc tgttccgcaa cgctggcgac agcaacgagt tcttcgcgaa gagctacggc
     2701 tacgccgtgt tccccaccat cggcaaaggt ggcttcggca tcggcggcgc ccggggcaaa
     2761 ggccgggtct acgtcggcgg caatcacgtg ggcaacacca ccatgacaca gctcaccatc
     2821 ggctttcaga tcggtgggca gggtttcagc cagatcatct tcttccagga cgagcgttcc
     2881 ttccgcgagt tctccaccgg caacttcgag ttcggagcca acgccagcgc cgtggccatc
     2941 accgcgggcg catcggcaag cgcgtccacc tcgggagcga cggcgggggc aagcggtggc
     3001 aagaaggatg cgaccaccgc cggcacggcc tactacaagg gcatgaaggt gttcaccatc
     3061 gccaagggtg gtctgatgta cgaagccagc atcggcggcc agaagttctc ctaccgaccg
     3121 gccggcggcg accagtcctg atctgactac cgctaccagc tggcatcgcg cgacggcccg
     3181 tcgccgcgcc ctcgtcaact cgactgcacg cccgccgccc agtccagcgg tggcaccctc
     3241 tg
//
LOCUS       DBNHLM010000138         3694 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_677404, whole genome shotgun sequence.
ACCESSION   DBNHLM010000138 DBNHLM010000000
VERSION     DBNHLM010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_677404"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..351
                     /gene="nrtS"
                     /locus_tag="AC16QF_06525"
     CDS             121..351
                     /gene="nrtS"
                     /locus_tag="AC16QF_06525"
                     /inference="COORDINATES: protein motif:HMM:NF038050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NrtS"
                     /protein_id="HIR6448245.1"
                     /translation="MSTQHGFLRLAARPDVVARAARTALLVGVILIAINHGDALLSGD
                     VGPVQAGKMALTVLVPYCVSTYASVRALQSAG"
     gene            complement(370..2466)
                     /locus_tag="AC16QF_06530"
     CDS             complement(370..2466)
                     /locus_tag="AC16QF_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor family protein"
                     /protein_id="HIR6448246.1"
                     /translation="MSGSSMIRALLAVAALCSATGAATGNEPTDTAGPALEELVVSAT
                     RTNRTVREVPAAVSVVGREDIQLGRQQLGLDEALGRVPGLFSQNRYNFAQDLRLSIRG
                     FGARANFGIRGLKLIADGIPATTADGQSGVDDIDLGSLDRIEVIRGPSSSLYGASSGG
                     VINLMTEDGPERPFVEAGVTLGEYDQQRYQLKAGGQTGRLNYLASASHLAYDGYREHS
                     RVESTIFNSKFRYGIDSASNLTVIVNLVDSPTADDAGAITQAQVEDDPRQAQQRNLSS
                     NAGESIEQQKLGIIYDRALDDRNAITLRNYYVWRDFQAFLPIGTHIPFVADDGVVEFD
                     RFFWGGGIQWRFADSLFGRPFQMSAGVDADIQEDDRQRYLNEAGVKGALAFDQLEKAE
                     AYGAYLRGEIGLADSVRLALGIRWDTVDLSVDDSFLDNADQSADLDFDELSPSVGLVW
                     DLGENWSAYANYATAFETPTFTELAVPARDLDVSLGGFANVSAQEAQSFEAGLRGGLL
                     DGALYVEAAAFTMEVDDEITSVSNIGNRSFFANADTDRRGIETFANLAFEGGLELSLA
                     YTYSDFEFDRFEDNEDFEGNALPGLPEHQLFAEIKYRHGSGAYAVADVLHVSKLYVNN
                     ANSGTSEASTVANLRVGASLALGQWQLDPFLGVNNLFDENYISNVRINGFGGRLFEPA
                     PKRNIYGGVAVRYVLR"
     gene            2691..>3694
                     /gene="ppnN"
                     /locus_tag="AC16QF_06535"
     CDS             2691..>3694
                     /gene="ppnN"
                     /locus_tag="AC16QF_06535"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007160674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HIR6448247.1"
                     /translation="MNTTTGQIRKVDVHVSPRGSLELLSQLEVNALASTAAESPLLDL
                     FRRCALAVLNTGSESDDAAAIFREYADFSIEVARRTRGVKLIIKNAPASAFVDGRMIE
                     GVRQHLFSVLRDIVYIGTDIADSDLFDLNSSEGITDAIFHILKNARVLRPDLPPRTVV
                     CWGGHSISRTEYEYTKEVGYHLGLRGLDICTGCGPGAMKGPMKGAAVGHAKQRQQDAR
                     YVGLTEPGIIAAEPPNPMVSDLVILPDIEKRLEAFLRIGHGVLVFPGGAGTVEEILYL
                     LGVLLDPANEGEEMPLIFTGPAESADYFFELDAFLRLSLGDEVARFYDIVIGDAEEVG
                     T"
BASE COUNT          619 a         1191 c         1232 g          652 t
ORIGIN      
        1 ccaacgcgga gacgggcagc ggctcgccga gccctccgaa tatgcctcga ttcgctgggc
       61 ctaccagcag gctctcgagc ccggagagtc tggcgcggta tcgttcgccc ttcgtctgcg
      121 atgagcacgc aacacgggtt tctgcgtctg gccgcccgcc cggacgtggt cgcgcgagcc
      181 gcccggacgg cgcttctggt cggggtcatt ctcatcgcca tcaaccacgg cgacgcgctg
      241 ttgtccggag acgtcggccc ggtgcaggcc ggcaagatgg cgctgaccgt gctggtgcct
      301 tactgcgtat ccacctacgc cagcgtcaga gcgctgcaga gcgcggggta atcggcgctc
      361 cccgaaccat cagcgcagca cgtagcgaac ggccacgccg ccgtagatgt tccgtttcgg
      421 agctggctcg aacagcctgc cgccgaaccc gttgatgcgc acgttggaaa tgtagttctc
      481 gtcgaagagg ttgttgacgc cgagaaacgg atcgagctgc cactggccca gcgccaagga
      541 cgcgccgact cgaagattgg cgacggtgga cgcttcgctg gtgccggaat tggcgttgtt
      601 cacgtacagc ttgctgacgt gcagaacgtc ggcgacggcg taggcgcccg agccatgccg
      661 gtacttgatc tcagcaaaga gctgatgctc gggcagtccg ggaagggcgt tgccttcgaa
      721 gtcctcgttg tcctcgaacc gatcgaactc gaagtccgag tacgtgtacg ccaggctcag
      781 ctcgagcccg ccctcgaagg cgaggttggc gaaggtctcg atcccgcgcc ggtcggtgtc
      841 cgcgttggcg aagaaggacc gattgccgat gttggaaacg ctggtgatct cgtcgtccac
      901 ttccatggtg aaagcggctg cctcgacgta cagggcgccg tcgagcaggc cgccgcgcaa
      961 accggcctcg aagctctgcg cctcctgggc cgagacgttc gcaaagccgc caaggctcac
     1021 gtcgaggtct cttgcgggaa cggcaagctc ggtgaaggtg ggtgtctcga aggcggtggc
     1081 gtagttcgcg taagcgctcc agttctcgcc cagatcccag accaggccga ccgaggggct
     1141 caactcgtca aagtccaggt ccgcggactg gtctgcgttg tccaagaaac tgtcgtccac
     1201 cgacaggtcg accgtgtccc agcgtatccc cagggcaagg cggaccgaat cggccagtcc
     1261 gatttctccc cgcaggtagg cgccgtaggc ttcggccttc tccagctggt cgaaggccag
     1321 ggcccctttt acgccggcct cgttgagata gcgctggcga tcatcctcct ggatgtccgc
     1381 gtccacccca gcgctcatct ggaacggccg gccgaacagc gaatcggcga agcgccactg
     1441 gattccgccg ccccagaaga agcggtcgaa ctccaccacg ccgtcgtcgg cgacgaaggg
     1501 gatgtgggtg ccgatgggca gaaacgcctg gaaatcgcgc cagacgtagt agttgcgcag
     1561 cgtgatcgcg ttgcggtcgt cgagagcgcg gtcatagatg atgccgagct tctgctgttc
     1621 gatggattca ccggcgttgg agctcaagtt cctctgctgc gcctgccggg gatcgtcctc
     1681 gacctgggct tgggtaatgg cgccggcgtc gtctgccgtc ggcgagtcga ccaggttgac
     1741 gatcaccgtg aggttcgacg ccgagtcgat cccgtaccgg aatttggagt tgaagattgt
     1801 cgactccacc ctcgagtgct cgcgataccc gtcgtaagcc agatgcgagg cgctggccag
     1861 gtagttcagc cgtccggtct gaccgcccgc tttgagctgg tatcgctgct ggtcgtactc
     1921 gccgagggtg acgccggctt cgacgaacgg acgctccggg ccgtcctccg tcatcaggtt
     1981 gatgacgccg ccggaggacg cgccgtacag cgaggacgac ggtccgcgga tcacctcgat
     2041 gcggtcgagc gaccccaggt cgatgtcgtc gacgccactc tggccgtcgg ccgtggtcgc
     2101 agggatgccg tcggcaatca gcttcaggcc gcggatgccg aaattcgccc tggcgccgaa
     2161 tccgcggatg gacagacgca ggtcctgggc gaagttgtag cggttctggg agaaaagtcc
     2221 cggaacccga ccgagggcct cgtcgaggcc gagctgctgc cgcccgagct ggatgtcctc
     2281 ccggccaacc acgctgacgg cagccgggac ctcgcgaacc gtgcggttcg tgcgtgtcgc
     2341 gctgacgacg agctcctcca gcgcggggcc agcggtgtcc gtcggctcgt tgccagtggc
     2401 ggcgccggtc gcgctgcaaa gcgcggccac ggcgagcagt gcacggatca tgctggaccc
     2461 ggacatcgat cctcctcttc cgatcgaaat tctcttgggc tggggcacac ggctttcccg
     2521 ggccgatcgc cttcgaatcg accgtgcccg caagcgcgtc aggccggacg cgcgccccgc
     2581 gggtacacta tcagaatccg ctttgggtta ccgcccgatg cgggccggct cggccggcgc
     2641 ggcgcatcga gcggtgggtg tcggcagcgc gaatcggtag acttcccggc atgaacacca
     2701 caaccggtca gattcgcaag gtcgacgtcc acgtcagccc gaggggatcc ctggagctgc
     2761 tctcgcagct cgaggtcaac gcgctggcca gcaccgcggc cgagtcgccg ctgctcgatc
     2821 tgttccggcg ctgcgcgctg gccgtcctga acacgggcag cgaatccgac gatgctgccg
     2881 ccatcttcag agagtacgcc gacttcagca tcgaggtcgc ccgacgcacg cggggcgtca
     2941 agctgatcat caagaacgcg ccggcttccg cgttcgtcga tggccggatg atcgaggggg
     3001 tgcgtcagca cctcttctcg gtgctgcggg acatcgtcta catcggtacg gacatagcgg
     3061 acagcgatct tttcgatctg aacagctccg aggggatcac ggatgcgatt ttccatatcc
     3121 tcaagaatgc ccgggttctg cgtcccgacc tgccgccgcg caccgtggtc tgctggggcg
     3181 gccactcgat ttcccggacc gagtacgagt acaccaagga agtcggctac cacctcggtc
     3241 tgcggggcct agacatctgc acgggctgcg gtccgggggc catgaaggga ccgatgaagg
     3301 gcgcggcggt cggccacgcc aagcagcgcc agcaggacgc tcgttacgta ggcctcacgg
     3361 agccgggaat cattgccgcc gagccgccga accccatggt gagcgatctg gtaatactgc
     3421 ccgacatcga gaaacggcta gaggccttcc tccgcatcgg tcacggcgtg ctggtgtttc
     3481 cgggcggcgc gggcaccgtg gaggaaatcc tctacctgct cggcgtgctg ctcgatcccg
     3541 ccaacgaggg cgaggagatg ccgttgatct tcaccggtcc cgcggagagc gcggactact
     3601 tttttgagct cgatgccttc ctcaggcttt cgctgggcga cgaggtggcc cgcttctacg
     3661 acatcgtgat aggcgacgcg gaggaggtgg gcac
//
LOCUS       DBNHLM010000139         7748 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1156227, whole genome shotgun sequence.
ACCESSION   DBNHLM010000139 DBNHLM010000000
VERSION     DBNHLM010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7748)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7748)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7748
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1156227"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..1038)
                     /locus_tag="AC16QF_06540"
     CDS             complement(226..1038)
                     /locus_tag="AC16QF_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6448248.1"
                     /translation="MATPANQIDTDEVLYDVADHVATITINRPDAQNTISGPMLDALT
                     QRLLEANENNDVRAIIVTGSGRFFCAGLDLRGGGIAGGLSSQGAGPANLDLRNTPPTV
                     LHSLDTPTICALNGSAAGYGMDLALGCDIRIMADSAKMAAAFTARGVVPESGGTWILP
                     RLLGWSKAAEIIFTGRTLLADECKELGLVSHVVPADDIGDAARNLALEIAANAPLAVQ
                     ASKRMMRMGMNETFNDHVHHVYLQLLPLFRSEDFREGMASFMEKRKAEFKGQ"
     gene            1208..1981
                     /locus_tag="AC16QF_06545"
     CDS             1208..1981
                     /locus_tag="AC16QF_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448249.1"
                     /translation="MKRIILALSLMTAPLLAGAEEAAPTPMYVAATNLTVSGHPGAQN
                     RFETFIKRAAEAHAKLDTKRHWFAWQQEMGNPRSYTLARTFGSMTAMDETPMNPIAEA
                     FGPEEIAKLTPDHEVVASVERGVWVQRPDLAVMPDSPVGQRVIWLQATVRAGENERFE
                     AYVAKVAEATREVAPEVRYATFAPVFGSQNTYVFSVPTSYAQLDEAPVMSVSERLREA
                     FGEREARRLESERAATVESLVQLLVRPRPDLAYQPPAEE"
     gene            complement(2037..2828)
                     /locus_tag="AC16QF_06550"
     CDS             complement(2037..2828)
                     /locus_tag="AC16QF_06550"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6448250.1"
                     /translation="MSEIAPAVPSATILMLRDGPRGLEVFMVVRHHEIDFASGALVFP
                     GGKADPGDFDEGLLPRLDGADADPTLRGIQVAAIREAFEECGILLARATGEDGLIRGG
                     RLAELERYRNALHAGELSMLEFAEREDIRLACNELTHFAHWITPENLPKRFDTHFYLA
                     MAPADHLALHDGHESVDSVWITPAQAIADAESGTRTVIFPTLRNLAKLGFSDTAEHAV
                     AAAATDTVVPVLPSIEQRDDGTYICIPPEAGYDISEERVPQPRSG"
     gene            2907..3431
                     /locus_tag="AC16QF_06555"
     CDS             2907..3431
                     /locus_tag="AC16QF_06555"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6448251.1"
                     /translation="MPTLTTERLLLRPLDDSDISDLYEFWNDPEVRRYLWEDQIMSRD
                     AVAQIVADSVRRFQALGSGFFAIEMAANPGSLVGFCGHREFEDGEQVELLFGIVPEFW
                     GEGFVTEAAMEVLRHGFETCGIVRVIAATETPNQRSVRVLQRLGMVFEERRKHHGLDT
                     VFYSLTSEGFASSG"
     gene            3428..3712
                     /locus_tag="AC16QF_06560"
     CDS             3428..3712
                     /locus_tag="AC16QF_06560"
                     /inference="COORDINATES: protein motif:HMM:NF016351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HIR6448252.1"
                     /translation="MNVAMLQVVFHLSGCRSLKEKRQRLKGIRDRFGRSSNVAVCESA
                     YQDSLQRAEWSFVASASSRAVVEKSLAEIERSLSLSVDAELVDVRRRWLV"
     gene            3672..4706
                     /locus_tag="AC16QF_06565"
     CDS             3672..4706
                     /locus_tag="AC16QF_06565"
                     /inference="COORDINATES: protein motif:HMM:NF022723.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3080 family protein"
                     /protein_id="HIR6448253.1"
                     /translation="MQSLWTSAVAGWCRRFALAGGVLVGVQCSGPPDNAFADYLSRVQ
                     RLLETDVSVDAEGSLPRYPNRGLLRRDVASLSIDVLDFIELHDCDLAGVVGFRNSPLG
                     RLQTPSQRLGYESEWLAGARSCASDGVGWLEPLIAKKEAALPAVFWNATFAGEEFARL
                     LGRFEASETPGAGYLLRELGDAYATLATDRFDLGGFERVLGALGSIPWLGQARRAWDL
                     ERRHLDAVAGALTEGLEGVCRNGAPTPKSRALVNVFQRYYVDALQPGIARRHGTDRDA
                     VAAVAALVGLFGGDVPGEFAVWYGQVLAPEPRRSEWSRLASVRLEHAAAWNRLFARCG
                     VDPARGLAQH"
     gene            4710..5114
                     /locus_tag="AC16QF_06570"
     CDS             4710..5114
                     /locus_tag="AC16QF_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448254.1"
                     /translation="MGSLLVVIAIACTTVLWVKGARKNRQRWLARLDLPGTWRWRPSD
                     SAESEVQGSLELSGGLSSGAYRLVEADVEEAGNWALEGHTLLLSPTGTASTGHYEIRF
                     FDEGKIGIDGPGRERRIYVKERTNVVPLRRRG"
     gene            complement(5135..5281)
                     /locus_tag="AC16QF_06575"
     CDS             complement(5135..5281)
                     /locus_tag="AC16QF_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448255.1"
                     /translation="MGERTGEDQRKGPKDRRTAAQDRRNAERMAEDLNPRRDPERRGR
                     RTGD"
     gene            5450..6337
                     /locus_tag="AC16QF_06580"
     CDS             5450..6337
                     /locus_tag="AC16QF_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6448256.1"
                     /translation="MSRILEELVADARELARRALQLVPAAPSVDWENTLAASWRETSW
                     GSGFAAHSELDDIELDDLLCVDDQKRSLDLNTQQFLAGAPANNVLLWGARGTGKSSLI
                     HALLNHYHARGLRLVEVSKTRLSGLPEIVLRLAAEPYRFVLFCDDLSFEADDASYKEL
                     KSALEGSVFKASSNVLIYATSNRRHLLPEYMSDNAQSGFVEGELHQSEAVEEKISLSD
                     RFGLWLSFYPFKQDAYLDVVRHWLGRLASEHDAEFVWDDEVRAEALRWALARGVRSGR
                     TAQHFARHWVGRLIINSKQ"
     gene            6437..7375
                     /locus_tag="AC16QF_06585"
     CDS             6437..7375
                     /locus_tag="AC16QF_06585"
                     /inference="COORDINATES: protein motif:HMM:NF016241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HIR6448257.1"
                     /translation="MHLKNGDRLSGTVDSIDGKRVVLDTDVAGKVVLKLSSVLNIETE
                     ESFDVKLKSGTKATGRFAVDDGAQTIEAQEEAPLPVALEEIRSAGQDRIAFVNLGSDW
                     STRLDLAAAISSGNSETESFNTVLESKLTRGRSEHGLYARISSEESENEKTKDQLDVK
                     YGYKRFFAEKWFGSGNVEYFEDGLKGIDHRITTGAGVGYQFWDDSFGALSVEAGGNWV
                     FEELFGEKEDNPAFRWALKFNRHLWSKRVEVFHNHSVLVIPDRGEVFDSSTGLRLAMT
                     DRVDSNFRVDLQHETDPPDGSEKTDVTYSIGVGVKF"
     gene            7469..7544
                     /locus_tag="AC16QF_06590"
     tRNA            7469..7544
                     /locus_tag="AC16QF_06590"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7503..7505,aa:Glu,seq:ttc)
BASE COUNT         1322 a         2333 c         2646 g         1447 t
ORIGIN      
        1 cagtcaagtg cgctaccaga ctgcgctacg ccccgaaagg tctggattta tccgcctggc
       61 ccggtgcgat ctgtggtgcg caatccgagg ccagagggcg cgaaatgata cctgaagggt
      121 aggagccgta aaagcgaacg attgacggct gctaccccgc cccacccgag acgcacctgg
      181 acttgtgggc ctatcgcaaa gagcatgcgc ccgggcgcga ggcggctact gtcccttgaa
      241 ctcggccttg cgcttctcca tgaaggaagc catcccttcg cggaaatcct cggagcggaa
      301 aagtggcagc agctgcaggt acacgtggtg gacgtggtcg ttgaacgtct cgttcatgcc
      361 catccgcatc atccgcttgg aggcctgcac ggccagaggc gcattggcgg cgatctcgag
      421 tgccaggttg cgggccgcgt ccccgatgtc gtcggcgggc acgacgtggg agaccagtcc
      481 gagctccttg cattcatcag cgagcagggt gcgccccgtg aaaatgatct cggccgcctt
      541 cgaccagccc agaagacggg gcagaatcca ggtcccgcct gattcgggaa ccacgccccg
      601 tgcggtgaaa gcggcggcca tcttggcaga atccgccatg atgcggatgt cgcagcccag
      661 cgccagatcc atcccgtagc cggccgcgga gccgttcagc gcgcagatgg tgggggtgtc
      721 caggctgtgg agaaccgtcg gcggcgtgtt gcgcaggtcg aggttggcgg gccccgctcc
      781 ctgggaagaa agcccgccgg cgatgccgcc gccgcgcaga tccagacccg cgcagaagaa
      841 gcgaccgctt cccgtcacga tgatggctcg aacgtcgttg ttctcgttgg cttcgagcag
      901 gcgctgcgtc agcgcgtcga gcatcggccc ggagatggtg ttctgtgcgt ccggacggtt
      961 gatggtgatg gtggcgacgt gatctgccac gtcgtagagc acctcgtcag tgtcgatctg
     1021 gttggcaggc gtggccattg ctttctttcc ccgaacggtt cgtcaggtgt gcaaactaat
     1081 ctttcgatct ctatcccgca agttggtgct aagctccggg atagttgggc agatgcgcgc
     1141 cgtttgcagg tggtggtcgt gtttgcaccc ggactgacac aaccaaagca tacgaggacg
     1201 agcgatcatg aaaagaataa ttctggcgct gagcctgatg acggcaccgc tgctggcggg
     1261 tgcggaagag gccgcgccga cgcccatgta cgttgccgct accaacctga cggtgagcgg
     1321 tcatcccggc gcgcagaacc gtttcgagac cttcatcaag cgggcggccg aagcccacgc
     1381 caagctggac acgaaacggc attggttcgc ctggcagcag gagatgggca acccgcgctc
     1441 ctacacgctg gcgcgcacgt tcggcagcat gaccgccatg gacgagacgc cgatgaaccc
     1501 gatcgccgag gctttcggcc cggaagaaat tgccaagctc acgcccgacc atgaggtagt
     1561 ggccagcgtc gagaggggtg tttgggtgca acggcccgat ctggccgtca tgccggattc
     1621 gccagtgggg cagcgggtga tctggctgca ggcaacggtg agagcgggcg agaacgagcg
     1681 ctttgaagcc tatgtggcca aggtggcgga agccacccgg gaagtggcgc ctgaggttcg
     1741 ctacgcgacc tttgcgcccg tcttcgggtc ccagaacacc tacgtcttct ctgtgcccac
     1801 cagctatgcc cagctggacg aagcccctgt catgtccgta tcggagcggc ttcgcgaggc
     1861 gttcggcgag cgcgaggcgc gacgtctcga gtcggagcga gcggcaaccg tggaatctct
     1921 ggtccagctg ctggtccgac ccagacccga cctggcctat cagccaccgg cggaggagta
     1981 gcacccagcc ggcatgggcg cccggtcgtt ggcgggtgcc cgtgcatcgc tgttgctcag
     2041 cctgatcggg gctgcggaac gcgctcttcg gagatgtcgt agcctgcctc gggcgggatg
     2101 cagatatagg tcccgtcgtc gcgctgctcg atgctcggga gaaccggcac tacggtgtcc
     2161 gttgcggccg cagcaaccgc atgctcggcg gtgtcgctga atcccagctt ggcgaggttg
     2221 cgaagcgtcg ggaagatgac ggtccgggtg ccgctttccg catcggcgat cgcctgggcg
     2281 ggcgtgatcc agaccgaatc cacggactcg tgaccgtcgt gcagggccag atgatcggcg
     2341 ggagccatgg ccagataaaa gtgggtgtcg aatcgctttg gcaggttctc cggggtgatc
     2401 cagtgagcaa agtgggtcag ctcgttgcag gcgagacgga tgtcttcccg ctctgcaaac
     2461 tccagcatcg acagctcgcc ggcgtgcagg gcgtttcggt accgctccag ctcggcgagc
     2521 cggccgccgc gtatcagccc gtcttcgccc gtcgccctgg cgagcaggat gccgcactcc
     2581 tcgaaggcct ctcggatggc cgcgacctga atgccgcgca gggtagggtc cgcatccgcg
     2641 ccgtccagcc ggggcagcag gccctcgtcg aaatcgccgg gatccgcctt cccgccaggg
     2701 aaaacgagcg cgcccgaggc gaagtcgatc tcgtggtggc gaaccaccat gaagacctcc
     2761 agcccccgcg gtccgtcgcg cagcatgagg atggtggccg acggcacggc cggggcgatc
     2821 tcgctcatcg tgtctccctc gaagggccgg gatgttcgct tatgtcccgg gctatggcaa
     2881 actcgggcgc gggagggagg tttcacatgc cgacgctgac gaccgagcgg ctgttgctca
     2941 ggccgctgga cgactcggat atctccgatc tgtacgagtt ctggaacgac ccggaagtgc
     3001 gccgctacct ctgggaggac cagatcatgt cgcgcgacgc ggtcgctcag atcgtggcgg
     3061 acagcgtgcg ccggttccag gcgctcggct ccggcttttt cgccatcgaa atggccgcca
     3121 atccgggctc gctggttgga ttctgcggtc accgggagtt cgaagacggc gaacaggtgg
     3181 agctgctgtt cggcatcgtg ccggagttct ggggagaggg attcgtgacc gaggcggcca
     3241 tggaggtgct gcggcacggc ttcgaaacct gcggcatcgt tagggtgatc gccgccacgg
     3301 agacgccgaa tcagcgatcc gtccgggttc ttcaaaggct cggcatggtt ttcgaggaac
     3361 gacgtaagca ccatggtctg gacacggtct tctactcact gaccagcgaa gggttcgcgt
     3421 cttccgggtg aacgtcgcga tgcttcaggt ggtcttccat ctcagcgggt gtcgctccct
     3481 caaggaaaag cgtcagcgtc tcaaggggat ccgggaccgg ttcgggcgaa gctcgaacgt
     3541 ggccgtatgc gaaagcgctt accaggactc cctgcagcga gcagagtggt cgttcgtggc
     3601 gtcggcgagc agccgagccg tggtcgagaa gagccttgcc gagatcgaac gcagcctgag
     3661 tctcagcgtc gatgcagagc ttgtggacgt ccgccgtcgc tggctggtgt aggcgcttcg
     3721 cgctggccgg gggtgtgctg gtcggcgttc agtgcagcgg cccgccggat aacgcctttg
     3781 ccgactacct gtctcgcgtt cagcgcctgc tcgaaaccga cgtttccgtg gacgctgaag
     3841 gctccttgcc tcgttatccg aatcggggcc tcctgcgccg cgacgtggcg tcactgagca
     3901 tcgacgttct ggactttatc gagctgcacg actgcgacct ggccggggtg gtggggtttc
     3961 gcaacagccc gctggggcgt ttgcagacgc cgagccagcg cctgggctac gagtctgagt
     4021 ggcttgcggg cgctcggtcc tgcgcgtccg acggggtggg gtggctggag ccgctgatcg
     4081 cgaagaagga ggctgcgctt ccggccgtct tctggaacgc cacgtttgcg ggggaggagt
     4141 tcgcccggct gctgggtagg ttcgaggcgt ccgagacgcc gggggcgggc tacctgctgc
     4201 gggaactggg ggacgcctac gcgacgctcg ccaccgaccg gttcgacctc ggcgggttcg
     4261 agcgggtgct cggagcgctc ggctcaatcc cctggcttgg ccaggccaga cgggcgtggg
     4321 atctggagag gcgtcatctg gacgcggtgg ccggggcgct gaccgagggg ctcgaagggg
     4381 tgtgtcgaaa cggcgccccg acgccgaaga gtcgggcact cgtaaacgtc ttccagcgtt
     4441 actacgtcga cgccctgcag ccgggcatcg cgcggcggca tgggaccgac cgggatgccg
     4501 ttgctgcagt tgccgcgctc gtcgggctct tcggtggcga cgtgcccggc gaattcgccg
     4561 tctggtacgg acaggtgctc gcccccgagc ctcggcggag tgaatggtcc cgactcgcgt
     4621 cggtgcgcct tgagcacgcc gctgcctgga acaggctctt tgcgcgctgt ggagttgatc
     4681 cggcgcgggg cttggcgcag cattgagcca tgggttctct gctggtcgtc attgccatcg
     4741 cctgcactac cgtgctctgg gtgaaaggtg cccggaagaa ccggcagcgc tggcttgcgc
     4801 gcctggatct gcccggtacg tggcgctggc ggccgtctga ttccgccgaa tcggaggttc
     4861 agggcagcct cgagctgagc ggtggcctga gctctggagc ttatcggctg gtggaagccg
     4921 atgtcgaaga ggccgggaac tgggctttag agggacatac cctgcttttg agcccgaccg
     4981 ggaccgcgag cacggggcac tacgaaatcc gctttttcga cgagggcaag atcggaatcg
     5041 atgggcccgg ccgggagcgg cgaatctacg tcaaggagcg gaccaacgtg gtgccgctcc
     5101 gcaggcgcgg ctgaagcaag gcaagcccgt tcgtctagtc gcccgtccgg cggccccgcc
     5161 gctccgggtc gcgccgcgga ttgaggtcct cggccatgcg ctcggcgttg cgacggtcct
     5221 gggccgccgt gcgtcgatcc tttggtccct tacgctgatc ttctccggtg cgttcgccca
     5281 tgtttgcgca gttcgatggc tctggttgtg cttgcttgga atgggcccgt tcattccggg
     5341 cgcgaccccg aagaagtcta acatggatag atacatgcct caccgcacgt ggtcagtttc
     5401 cggtcggttt ctggttacaa ttgcgcccgc ttgaaaccct ggacttttcg tgagcagaat
     5461 cctggaggaa ctcgtcgccg acgcccgcga gctggctcgc cgggcgctgc agctcgtccc
     5521 ggcggcgccg tccgtggatt gggaaaacac cctggccgcc agctggcggg agacgtcatg
     5581 gggctcggga tttgctgcac attccgagct ggacgacatt gagctggacg atcttctctg
     5641 cgtggacgat cagaagcggt cgctggatct caacacgcaa cagtttctcg ccggtgcccc
     5701 ggccaacaac gtgctgctgt ggggtgcccg cggcaccggt aagtcgtcgc tcatccatgc
     5761 gctcctgaat cactatcacg cacggggatt gaggctcgtc gaagtcagca agacgcgcct
     5821 gtccggcctg ccggagatcg tcctgcgact ggctgcagag ccctaccgct tcgtgctgtt
     5881 ctgtgacgac ctctccttcg aggcggatga tgccagctac aaggaactca agagcgctct
     5941 ggaaggatcg gttttcaagg cgtcgagcaa cgtcctgatc tacgccacct ccaaccgccg
     6001 gcacctgctt cccgagtaca tgtcggacaa cgcccagagc ggtttcgtgg aaggcgaact
     6061 gcatcagtcc gaggcggtcg aagaaaaaat ctctctatcc gatcgcttcg ggttgtggtt
     6121 gtccttttat cctttcaaac aggacgccta tctcgatgtc gtcaggcact ggctcggcag
     6181 gctcgcaagt gagcacgacg ccgaattcgt gtgggacgac gaggtcagag cagaagccct
     6241 gcgctgggct ctcgctcgcg gagtgagaag tggccgaaca gcgcagcatt ttgcgcgcca
     6301 ctgggtcggc cggctcatca tcaacagcaa gcaataaggg aatcgtgtga accttctaga
     6361 agatcgaaag atccgggcag ctattgcctt ggggatactc agccttctgg ggacggcacc
     6421 ggcgggagcc gacgtcgtgc atctgaaaaa tggcgaccgg ctgagcggga cggtagacag
     6481 catcgacggc aagcgcgtcg ttctggacac cgacgtggcc ggcaaggtgg tgctcaagct
     6541 gagctcggtg ctcaacatcg agaccgagga gagcttcgac gtgaagctca agagcgggac
     6601 gaaagcgacc gggcgcttcg ccgtggacga cggggcccag accatcgagg cgcaggagga
     6661 ggcgcctttg cccgtggctc tcgaggaaat caggtccgcg ggtcaggatc ggatcgcgtt
     6721 cgtcaatctc ggcagcgact ggtccacccg gctcgatctc gcggctgcca tatcctccgg
     6781 taactcggaa accgagtcgt tcaacacggt gctcgagtcc aagctgacgc gcggccgctc
     6841 tgagcacggc ctctatgccc gcataagcag cgaggagtcg gagaacgaga agacgaagga
     6901 tcagctggac gtcaaatacg gctacaagcg gttctttgcc gagaagtggt tcggctccgg
     6961 caacgtggag tacttcgagg atggtctgaa gggcatcgat caccggataa ccacgggtgc
     7021 cggcgtgggt taccagttct gggacgacag cttcggcgcg ctctccgtgg aagccggcgg
     7081 caactgggtg ttcgaggagc tgtttggcga gaaagaggac aaccccgcct tccgctgggc
     7141 gctgaaattc aatcgccacc tctggagcaa gcgggtggag gtcttccaca atcactcggt
     7201 gctcgtcatc cccgaccgcg gtgaggtctt cgactcgtcc acgggactgc gcctggcgat
     7261 gaccgaccgg gtggacagca acttccgggt cgatcttcag cacgaaaccg atccgccgga
     7321 cggaagcgaa aagacggacg tgacctacag catcggcgtt ggggtcaagt tctgagcggg
     7381 cagatgggtg ccggcgggga gattgccttg gcatgccggc attgcttgac tggggtaggg
     7441 cggcttctta gaattccgcg ccttctaagt ccccttcgtc tagaggccta ggactccagg
     7501 ttttcatcct ggcaacaggg gttcgactcc cctaggggac gccaatcaaa acggcccgcc
     7561 ttgtgcgggc ttttttgatt gcgctcttct ttggcggagt gggttcgccg cagttttgcg
     7621 tagcgaaact gccgtaaggt cgcgaagcgg ccggcgggtt ccgactcccc taggggacgc
     7681 caatcaaaac ggcccgcctt gtgcgggctt ttttgattgc gctcttcttt ggcggagtgg
     7741 gttcgccg
//
LOCUS       DBNHLM010000140         3998 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_797021, whole genome shotgun sequence.
ACCESSION   DBNHLM010000140 DBNHLM010000000
VERSION     DBNHLM010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_797021"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1760
                     /locus_tag="AC16QF_06595"
     CDS             117..1760
                     /locus_tag="AC16QF_06595"
                     /inference="COORDINATES: protein motif:HMM:NF019042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprO/OprP family phosphate-selective porin"
                     /protein_id="HIR6448258.1"
                     /translation="MRNSILIATLLLAWWPQWVSAESEGEGADLVLRNVILPDPGDAS
                     ADVVINVVIKDGKALLVTKDEPPGAEGPVLEATGTYLLGRLHTGSAPSFLLLNADPRE
                     EFSVLLDTATHAVFAISDGVVMKNDLPVPAASPDAPRRRSGWLAYTPPPISLPTSYRD
                     TSKWNRWETPFISGLFVGAVVLDRQFWQSQDAASESQVGDLTPFDGGEIRGLRFGAIG
                     TLNFPKPWVYTIFGATNAFDKGFNADDTDDVTLFDLRLDIPLFGSTLSLGKQKEPISM
                     ERTMGLVYLPIQERSAAMDGMLPARNTGLVLSGNAFAQRVSWAGGVFNDWLDTAGSFD
                     EGANQVVGRLTWLAASSADDSNLLHLGAGVRYSDAREGFRFSTEPEFNQAPLYIDTSI
                     DGTLLAAEDLLTWNLEASWRYGPLWLAGEYVKTDVDAPQYADPSFSGHHITASWALTG
                     EMRSYRRKNGTFSPLPVARSVHNNGPGAWELAARWSVFDANSGPISGGKTDIFSLGLN
                     WWLTPLFSVGANYRWITLDRDGLTGESDGFSTRILLMLE"
     gene            1819..3015
                     /locus_tag="AC16QF_06600"
     CDS             1819..3015
                     /locus_tag="AC16QF_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448259.1"
                     /translation="MAILIKLMSRIAILGLLAAPAAQAETWRPVSDKDELASLFSDTT
                     MTTTLRDGVTATAEYRADGTGTVRAWGDAFERRWSVSADSEVCIEAGDQTFCYTVETD
                     ADATDRYRSTNLETGERLILMIARQGGADPEPTQGTDSGAAGKPSAEEVAAKLANPNT
                     PLASLTTKLQYRTFDGNLADAGGQDGTTLLLQPSFPFALANGDLILFRPAIPILLDQP
                     VPGDDGFGSKSGLGDIAFDLAYARTTDSGMLIAAGVISSLPTATKKALGTDRWTLGPE
                     LLIGKTTKKHVLGLFPNHQWDVAGSGDVDVSLTTIQAFYTHLPGGGWSVGTAPIMSYD
                     HIANQWTLPINLTVGKTVIWNGRPWKLSAEINYYVDQSDAFGPEWMIGINVAPVVENV
                     LAKWFR"
     gene            3049..3387
                     /locus_tag="AC16QF_06605"
     CDS             3049..3387
                     /locus_tag="AC16QF_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448260.1"
                     /translation="MNRKPANQLALVATTTLLSGAALADVFSEAAKLGANTGAMAYCG
                     DNFADDDDKGRYKMIRLKLLGEFGDLPDDEKAKALILKKAAEDDGDYLGKKLDADRCT
                     SLRKTLYLKY"
     gene            complement(3441..>3998)
                     /locus_tag="AC16QF_06610"
     CDS             complement(3441..>3998)
                     /locus_tag="AC16QF_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR6448261.1"
                     /translation="NFDLGSSIRTVGMGLSVEHDSRDLPLNSFSGRLFELRGLFNDSA
                     FGSDSTYQAYHAALRSYHLLTKNVVLAWELEACKKAGSVPLWDACRIGLRGFPATDYL
                     GKLSGSAQIELRWKFWKRWGLVGFAGGGGVDQSLNKEDDGEVVPSYGVGLRFMLLKSK
                     RINFRLDYGRSDEGDAVYFSVGEAF"
BASE COUNT          808 a         1307 c         1239 g          644 t
ORIGIN      
        1 tcactccgaa cctgcagctc ctgatcgacc ccgccctgaa tccggaagag gacgccatct
       61 gggtcttcgg cctgcgggca aaggggtcgc tttagccggg gcgataaggg gggaatatgc
      121 gcaactcgat cttgatcgcc acgctgctgc tcgcctggtg gccccaatgg gtcagcgcgg
      181 agagcgaagg agaaggcgcg gacctcgtgc tgagaaacgt gattctgccc gacccgggcg
      241 acgcatcggc ggacgtggtg atcaacgtcg tgatcaaaga cggaaaggcc cttctcgtca
      301 ccaaggatga gccccccggc gcggaaggcc cggtgctcga agccaccggc acctatctgc
      361 ttggcaggct gcacacgggc agcgcgccga gctttctcct gctgaacgcc gatccaagag
      421 aggaattcag cgtgctgctg gacaccgcca cccatgccgt tttcgccatc agcgacgggg
      481 tcgtcatgaa gaacgacctg ccggtcccgg cagcatcgcc cgacgcgcct cgacggcgct
      541 ccggttggct cgcctacaca ccaccgccca tctcgctgcc gacgagctac cgggatacca
      601 gcaagtggaa ccgctgggaa acgcccttca tctcgggcct gttcgtgggc gccgtggttc
      661 tcgatcgcca gttctggcag tcccaggatg cggccagcga aagccaggtc ggcgatctga
      721 caccctttga cggcggcgag atccgcggcc tgcggttcgg cgccatcggc acgctgaact
      781 tccccaagcc gtgggtatac acgatcttcg gcgccaccaa cgcgttcgac aaaggtttca
      841 acgccgacga taccgacgac gtgaccctgt tcgacctgag gctggacatt ccgctgttcg
      901 gcagcacgtt gagtctcggc aagcagaaag agcccatctc catggagcga accatggggc
      961 tcgtctacct gcccattcag gaacgttccg ccgccatgga cggcatgctg ccggctcgca
     1021 acacggggct ggtgctgagc ggcaacgcct tcgcccagcg cgtcagctgg gcggggggcg
     1081 tattcaacga ctggttggac accgcgggct cgttcgacga gggagcgaac caggtggtcg
     1141 gccggctgac ctggctggcg gcctcttccg ccgatgacag caacctcctt cacctgggcg
     1201 caggcgtgcg ctacagcgac gccagggaag gatttcgctt ctccaccgag ccggagttca
     1261 accaggcgcc cctgtacatc gatacgagca tcgacggaac tctactggcc gcggaggacc
     1321 tgctgacctg gaatctggaa gcatcctggc ggtacgggcc tctatggctg gccggcgagt
     1381 acgtcaaaac cgacgtggac gcaccgcaat acgccgatcc cagtttctcc gggcatcaca
     1441 tcaccgcatc ctgggctctg accggcgaga tgcgctcgta tcggcgcaag aacggcacgt
     1501 tcagcccgct gccggtcgcc cggtccgtcc acaacaacgg gccgggagct tgggagctgg
     1561 ccgcgcgctg gtcggtgttc gacgccaact ccgggccaat cagcgggggc aagacggaca
     1621 tcttcagcct gggcctgaac tggtggctga caccgctctt cagcgtcggt gccaattacc
     1681 gctggatcac cctggacagg gacgggctga ccggcgagag cgacgggttc tcgacgcgca
     1741 tcctgctgat gctggagtaa tcggggcccg acagagagac gattcggaga tcggataacg
     1801 atgcggagat cagacaacat ggcaatccta atcaagctca tgtcgcgcat cgcgatcctg
     1861 ggccttctcg cagcgcccgc tgcgcaggcg gagacctggc gcccggtcag cgataaggac
     1921 gagctcgcca gcctcttcag cgacaccacc atgaccacga cgctgcgcga cggcgtcacc
     1981 gccaccgcgg agtatcgtgc ggacggcacc ggaaccgttc gggcctgggg cgacgctttt
     2041 gagagaagat ggtcggtcag cgccgatagc gaggtctgca ttgaggcggg cgatcagact
     2101 ttctgctaca cggtggagac cgacgccgac gccaccgatc gatatcgatc aacaaacctg
     2161 gagaccggcg agcggctcat cctgatgatt gcccgccagg gcggtgcgga tccggagcca
     2221 acccaaggga cagactccgg agcggcggga aagccgtcgg ccgaggaggt cgccgcgaag
     2281 ctcgccaacc caaacacgcc gcttgcgagc ctgaccacca agctgcagta ccgcaccttc
     2341 gacgggaacc tcgcggacgc cgggggtcag gatggcacca cccttctgct tcaaccgtcc
     2401 tttccgttcg cgctggccaa cggggatctg attctctttc gtcccgccat acccattctc
     2461 ctggaccagc cggttccggg ggacgacggg ttcggctcca aatccggtct tggcgacatc
     2521 gccttcgacc tggcgtatgc caggacgacg gacagcggca tgctcatcgc cgcgggcgtg
     2581 atctcgtccc tgccaacagc cacgaagaag gcactgggca ccgaccgctg gaccctgggt
     2641 cccgagctgc tgatcggaaa gaccaccaag aagcacgtcc tggggctgtt ccccaaccat
     2701 caatgggacg ttgccgggtc cggcgacgtg gacgtcagcc tcaccaccat ccaggctttc
     2761 tacacccatc tacccggcgg cggatggagc gtgggcacgg cgcccatcat gagctacgac
     2821 catatcgcca atcagtggac gctgccgatc aacctcaccg tcgggaagac ggtcatctgg
     2881 aacgggcggc cgtggaagct gagcgccgag atcaactact acgtcgatca gagcgacgcg
     2941 tttggaccgg aatggatgat cggcatcaac gtagcacccg tggtcgagaa cgtgctcgcg
     3001 aagtggtttc gctagccaac acagcaaccc aaccaggagg atcagaccat gaacagaaaa
     3061 cccgccaatc aactcgcgct ggtcgcaaca accacgcttc tcagcggcgc ggcgctcgcc
     3121 gacgtatttt cggaagcggc aaaactcgga gcgaacaccg gggccatggc gtactgcggc
     3181 gacaacttcg cggacgacga tgacaaaggc cggtacaaga tgatccggct caagctgctc
     3241 ggcgagttcg gcgaccttcc ggatgatgag aaagccaagg ctctcattct caagaaagcc
     3301 gccgaggacg acggggacta cctgggcaag aagctggacg cggatcgctg cacgagtctg
     3361 cggaagaccc tttacctcaa gtactgacgg tcataacgaa gcaacggggg cccaggcccc
     3421 tcattgccga gcgaggcttc ttagaacgcc tcgccgacag aaaagtaaac ggcgtcccct
     3481 tcatcgctgc gcccgtagtc cagccggaag ttgatgcgct tcgacttgag cagcatgaag
     3541 cgcaagccca cgccgtagct ggggacaact tccccgtcgt cttccttgtt tagagactga
     3601 tccacgcccc cgccaccagc gaagcccacc agcccccagc gcttccagaa cttccaccgc
     3661 agctcgatct gggcagagcc ggagagcttg cccaggtagt cggtagcagg aaatccacgc
     3721 aagccgatgc ggcaggcatc ccacaacggc acggatccgg ccttcttgca ggcctccagt
     3781 tcccaggcca gaactacgtt cttcgtaaga aggtggtagg agcgcagggc ggcatggtaa
     3841 gcctggtagg tcgaatcgct gccgaacgcc gagtcgttga acagcccccg cagctcgaag
     3901 agccggcccg agaaggagtt cagcgggagg tcccggctgt cgtgttccac gctgaggccc
     3961 atcccgacag tgcgaatact cgagcccagg tcaaagtt
//
LOCUS       DBNHLM010000141         3109 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1217446, whole genome shotgun sequence.
ACCESSION   DBNHLM010000141 DBNHLM010000000
VERSION     DBNHLM010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1217446"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="AC16QF_06615"
     CDS             complement(<1..381)
                     /locus_tag="AC16QF_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448262.1"
                     /translation="MLRRNTVWLVLVATGLLASTAYPPEAAARVTLSNTVARYEIAQG
                     LDGQVERRLVAADEVLPGDELLYTITFANEGREEVAAGSIVITNPIPEVAEYVEGSAA
                     GEDTFITFSVDGETFAEPSGLMVQR"
     gene            complement(474..719)
                     /locus_tag="AC16QF_06620"
     CDS             complement(474..719)
                     /locus_tag="AC16QF_06620"
                     /inference="COORDINATES: protein motif:HMM:NF018500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HIR6448263.1"
                     /translation="MGCGSGHENDGAAIKVPLSELSEEALAGVVESFVLREGTDYGHR
                     EFTLAEKRALVRKQLESGDAVIYFDPLTRSIDIKPAR"
     gene            807..1514
                     /locus_tag="AC16QF_06625"
     CDS             807..1514
                     /locus_tag="AC16QF_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008093405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6448264.1"
                     /translation="MAEPDHILVVEDEPITREQLVSYFEEEGFRVTSTGSGDEVHELV
                     KNSDIILVLLDIKLPGKDGLTLTREIRAQSDVGIILVTSKQEQIDRILGLESGADDYV
                     TKPFDPRELLSRARNLIRRVQLQQRQRRKNHIRNFEGWQLDLNKRELTSPEGEKTTLS
                     AGEYQLLLAFMEQAGEVMNRDQLMNRIRNREWYPDDRYIDVLVGQLRKKLGERAANAK
                     IIATIHGTGYLFTPEVA"
     gene            1581..>3109
                     /locus_tag="AC16QF_06630"
     CDS             1581..>3109
                     /locus_tag="AC16QF_06630"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR6448265.1"
                     /translation="MSSVDEKIYESASTVVRRTTWQDQPVVAKRLKPEGLPSNVLSQF
                     HHEFHLNQSLTSPNVCRALAMDDSGLEIYFEDTQGASLRSLIDTGELSLDERIAIAVE
                     LSRALQSVHDEGVIHRDVNPGNVLVSEDPMRVHLIDFGLATLAAREYPEVEPLGQVTG
                     TLPYLSPEQTGRVNRVVDYRTDLYSLGATLYELLAGNPPFTNTDPLELIHAHIASTPR
                     PLTAIGEGIPRWLSEIVQKLLAKQPEDRYQSAAAVRDDLIRGQTQANVVPFRLGQTDS
                     PGLLAMPKRLYGRTAQIGIIQEYLERAARGEVLFLQITGAQGRGKSSLCAELARQAAG
                     TKAMVARTNALAFDFRDTDAVWIELTRQLLRQALADAKAGDLLARIAALNSPNVRSLT
                     EIIPELRDLVPAGSEGAGLPGVGIQEVLKALQPQPLCLIVEEVDNVPSESLETLLSMA
                     LDSRHLLMTVTRANADSDAFRTPRLATKAATLELPAFARADVRSLLSDMLSTSEVRVR
                     EL"
BASE COUNT          621 a         1037 c          932 g          519 t
ORIGIN      
        1 gcgttggacc atcaggcccg acggctcggc gaaggtttct ccatcgaccg agaaggtaat
       61 gaaggtgtcc tcacccgccg cgctgccctc cacatactcg gccacctcgg gaatcgggtt
      121 ggtgatgacg atgcttcctg cggccacctc ctctcgcccc tcgttggcaa acgtgatggt
      181 gtagagcagc tcgtcgccgg gaagcacctc gtccgcggcc acgagccgtc gctcgacctg
      241 cccgtccagg ccctgcgcga tctcgtacct ggccaccgtg ttggagagcg ttacgcgcgc
      301 tgctgcttca ggggggtatg ccgtcgaggc gaggaggccg gtggcaacga gcaccaacca
      361 gaccgtgttt cgcctcagca cattcaagat cacacttcct ccagagcctt ccggcacgct
      421 tggaccctcc gttccgggaa tcgatgttct cacacccagc gcatcgcaga ccattaccga
      481 gcaggtttga tgtcaatgct gcgggtgaga gggtcgaagt agatgaccgc atcaccactt
      541 tcgagctgct tgcggaccag cgcgcgcttc tccgcgagcg tgaattcccg atgtccgtag
      601 tcggtgccct cgcgcagcac gaacgactcg acgacgcccg caagggcttc ctccgagagc
      661 tccgacaggg gaactttgat cgccgcccca tcattttcgt gacctgatcc gcatcccatg
      721 tcgctcgcct tgaatttctg aacctaaggt gcgttacttc cccttactga aatcgtcata
      781 ccggcgttcc aacaggacgg ggcaccatgg ccgaaccaga tcacatactc gtcgtcgaag
      841 acgagccgat tacccgcgag caactcgtat cctacttcga ggaagaaggt ttccgcgtca
      901 cttcgaccgg atccggcgac gaggtgcacg agctggtaaa gaacagcgac attatactcg
      961 ttctcctgga catcaagctg cccggcaagg acggtctgac gttgacccgc gagatccgcg
     1021 ctcaatccga cgtcggcatc atcctggtaa caagcaaaca ggaacagatc gaccgcattc
     1081 tcggtctgga gagcggcgcc gacgactacg tgacgaagcc cttcgacccg agggagctgc
     1141 tcagccgcgc ccgcaacctg attcggcgcg tccagcttca gcagcgccag cgccgcaaga
     1201 accacatccg caacttcgag ggctggcagc tagatctgaa caaacgcgag ctgacttctc
     1261 cggaaggcga gaagacgacg ctgtcggcag gcgagtacca gctcctgctg gcattcatgg
     1321 agcaggccgg cgaggtcatg aaccgcgatc agctgatgaa ccgcatccgt aaccgcgagt
     1381 ggtatcctga cgaccgatac atcgacgtgc tggtcggcca gctccgcaaa aagctcggcg
     1441 agcgggcggc caacgccaag atcatcgcca ccatccacgg gacgggatac ctgttcacac
     1501 ccgaagtggc ctgaattcgt tcagcaaacg ggatgtgacg atcctgaaca gcgtgtgggg
     1561 gcacagacgg gggaaaaggc gtgagcagcg tggatgaaaa gatctacgaa agcgcgtcga
     1621 cagtcgtccg acggaccacc tggcaggatc agcccgtcgt tgcgaaacgg ctgaagcccg
     1681 agggcctgcc ctcgaacgtg ctcagccagt ttcatcacga attccacctg aatcagtcgc
     1741 tgaccagccc caacgtctgt cgcgcgctcg ccatggacga cagcggcctc gagatctact
     1801 tcgaggacac ccagggcgcc tctctgcgca gcctcatcga caccggcgag ctttccctgg
     1861 acgaacgcat cgcgatcgcc gtggagctca gccgcgcgct gcagtccgtt cacgacgagg
     1921 gcgtgatcca cagggacgtc aaccccggca acgtgctcgt ctccgaagac cctatgcgcg
     1981 tgcacctcat cgacttcggc ttggctacgc tggcagcccg ggaatatccc gaggtggaac
     2041 cccttggcca ggtgaccggg accctgccct acctttcacc cgagcagacc ggccgcgtga
     2101 accgagtggt ggactaccgc acggacctgt actcgctggg cgcgacgctc tacgagcttc
     2161 tggccggcaa ccccccgttc accaacacgg acccgctgga gctcatccac gcgcacatcg
     2221 cgagcacgcc gcggccccta accgccatcg gcgagggaat tccgcgctgg ctgtcggaaa
     2281 tcgtccagaa actgctcgcc aagcaaccgg aagaccgcta ccagagcgcc gcagccgtcc
     2341 gcgacgatct gatcaggggg cagacccagg ccaacgtggt accgttccgg ctcggacaga
     2401 ccgactcgcc cgggctgctg gccatgccca aacgcctgta cggtcgcacc gcccagatcg
     2461 gcatcattca ggaatatctg gagcgcgccg cacgagggga agttctcttc ctgcagatca
     2521 ccggggcgca aggcagagga aagtcctccc tatgcgcgga gctcgccagg caggcagccg
     2581 gcacaaaagc catggtcgcc cggaccaatg cgcttgcctt cgactttcgc gacaccgacg
     2641 cggtctggat cgagctgacc cgccagctcc tgcgacaggc gctggccgac gctaaagccg
     2701 gtgaccttct ggcgcgcatc gcggcgctga actcgccgaa cgttcgcagc ctgacggaga
     2761 tcatcccgga gctgagagat ctggtgcctg cgggcagtga gggcgcgggg ctgcccgggg
     2821 tgggcatcca ggaagtcctg aaagcgctgc agccccaacc gctttgcctg atcgtggagg
     2881 aagtcgacaa cgtcccttcg gaaagcctgg aaaccctgct gagcatggcg ctcgactcgc
     2941 ggcacttgct gatgacggtt acccgggcga acgccgattc ggacgcgttc aggacgcccc
     3001 gactggcgac caaggcggcc acgctggagc tgccggcatt tgcgcgggcc gacgtgagaa
     3061 gccttctgtc ggacatgctc tcgaccagcg aggttcgggt tcgcgagct
//
LOCUS       DBNHLM010000142         9632 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1636567, whole genome shotgun sequence.
ACCESSION   DBNHLM010000142 DBNHLM010000000
VERSION     DBNHLM010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9632)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9632
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1636567"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /gene="pgl"
                     /locus_tag="AC16QF_06635"
     CDS             complement(<1..623)
                     /gene="pgl"
                     /locus_tag="AC16QF_06635"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIR6448266.1"
                     /translation="MMPGIETFPDRSAASRAAAAVLAMALSRRLATSTEAVVAVSGGT
                     TPRQCLGELAAHPLDWARVRVTLTDERCVPADHPDSNEGMVRRRLLVGRAAAARFLPL
                     QSAELRAFSAPLACALVGMGEDGHFASVFPDADNLDEALDLESNACCLRVRTQASPSA
                     RVSMTLARLLESDELVLLAFGVSKRRVLEAPGAYPVGHLLAQTRLPVR"
     gene            complement(620..2083)
                     /gene="zwf"
                     /locus_tag="AC16QF_06640"
     CDS             complement(620..2083)
                     /gene="zwf"
                     /locus_tag="AC16QF_06640"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIR6448267.1"
                     /translation="MAQLIPVDPFDLIIFGGAGDLALRKLLPALYHRHRDGQIPPGSR
                     IIAVGRSAMGRAEYLSEAARAVEAAGEGREPDIWETFAARLDYLDLDADDHSGWGPLA
                     ECLRGHEGKIRIVYLATPPHLFGSVARGFQSNSLMVPDMRIVLEKPVGDDYASARAIN
                     DEVGACFAENQIFRIDHYLGKETVQNLLALRFGNSLFEPLWRREVVDHVQITVAEELG
                     VGNRVAFYDRIGAVRDMVQNHLLQLVCLVAMEPPASLDDDAVRDEKLKVLRALKPITA
                     ANVRTSTVRAQYTAGAISGHAVPGYVDELGGATDTETFVALKLGIENWRWSGVPFYLR
                     TGKRLPAKHSEIVIQFRPVPHPLFGDVSGDAAPNRLSIMLQPDEGVRLAIMAKEPGPG
                     GFELRPVSLDLSFEETFGIRYPDAYERLLIEVMRGNPALFMRRDEIETAWRWIDDIIA
                     GWAEARQKVDAYVSGSWGPTASSLLLDRDGRHWFAGQ"
     gene            2186..3046
                     /locus_tag="AC16QF_06645"
     CDS             2186..3046
                     /locus_tag="AC16QF_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR6448268.1"
                     /translation="MTTGILRQIDLDLPRLSRSETRIAEWIRENPRQTVDASIGEVAA
                     NAGVSEPTVIRFCRRLGLAGFRDLKTRLVAALQSPENFLHRDVDDADGPSEAAAKVLD
                     GSIRTLVDLREQTPTMPFRAAVETMTTARELVFVGLGASGHVARDASHKFFRLGIPCS
                     TAVDAQTILQRAAIASARDVFVVVSHTGTWPEIVTAMAIAAERHARIVALTDPRSPLA
                     TTADLVFACHPVEDTNVFTPMSSRLAQLALLDALQVALALGIGEPAKQNLRASKEALS
                     EMRASACGSN"
     gene            3054..3383
                     /locus_tag="AC16QF_06650"
     CDS             3054..3383
                     /locus_tag="AC16QF_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014886.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HIR6448269.1"
                     /translation="MDRISGRRIYCRVLTGGELGAGKGINKRGGGLSALADNPRTLAR
                     VALFRGVHPELFETEHLDYELINEAAVTWLAHKGAVSRGDRVILSKGDYRNVQGGTNT
                     LKILEVA"
     gene            3383..4399
                     /locus_tag="AC16QF_06655"
     CDS             3383..4399
                     /locus_tag="AC16QF_06655"
                     /inference="COORDINATES: protein motif:HMM:NF014818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HIR6448270.1"
                     /translation="MRKLRVGIMGFGRVGRQIYRLALKDERFEVVAISDIGRPEILHH
                     LLTRTDDDVGVTLSGNYLESRGVRTRLMPADHPTEIPWDVFGVDVVIEATGRFHRADD
                     LRPHLHNGAPRVVLSALPQDDIDRVVLVGVNDCEASAADVMVSAGSASTTATALACKT
                     ITEVWPVEHATMTSVHAYTSDQSLQDYAGPDYRRSRSGAENIIPNATPALKWVQRAVP
                     AMAGRMTGYALNVPVQVGSMLDLTFSFRESGIGADDVNDLFGNAARVRPDLIATTRDP
                     IVSSDVRGSSESLLVDMEGTMAAGSRMVKVLAWHETLGHARRILDVVGLYADLDDATA
                     KEAS"
     gene            4399..5400
                     /gene="gap"
                     /locus_tag="AC16QF_06660"
     CDS             4399..5400
                     /gene="gap"
                     /locus_tag="AC16QF_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR6448271.1"
                     /translation="MRVAINGFGRIGRSVFRIAETLGDIEIVAINDLFDDEALRYLLT
                     YDTVMGRFEGDVRIEDGHMITPKSRARMLALADPTQLPWAELEIDAVVESTGVFRSRE
                     QVSQHLEAGAGRALLTVPAKDDIDYTVVLGVNGEGLKAEHRVVSNASCTTNCLAPLAK
                     VLHDAFGIREGVINTVHAYTNDQRLADVPHSDWRRSRAAAENIIPTSTGAAKAVGVVL
                     PELAGRLDGIASRVPVPDGSVVDLFVELEKNVSVDDVHEVLEAAAGSEPMSGVLEFTR
                     EPIVSSDIIGNPHSSIYDASFTQVAGGRFLKALSWYDNEWGYSNRVCDVLRMMSALD"
     gene            5614..6144
                     /locus_tag="AC16QF_06665"
     CDS             5614..6144
                     /locus_tag="AC16QF_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448272.1"
                     /translation="MPKTPIIALLTALACLCWSPVSADDVSRKEMRSLDEQVQEIKSD
                     VLSIAAELDQLEERLLFPSNTQLTVFVAIGDGTESFRLDSIRLGINDALATHHIYSFK
                     ELEALQQGGVQRLYMGNLPIGTHALEVSVLGKLPSGKDFAHAERFTFDKGIDPALIGI
                     TVDPGSGKAPIALKDW"
     gene            6147..8027
                     /locus_tag="AC16QF_06670"
     CDS             6147..8027
                     /locus_tag="AC16QF_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6448273.1"
                     /translation="MTRPLAWTIGLQLALMLLCSPARADDVRDLYFGEALFQAGQGKY
                     FEALERLDAELGQHYGLDEPELDTLHHHIREAEFFVGDFELNYRMHHRAGRAISAVLN
                     GDVDQSVRNEAAYRLARIHFQKAQMADALEALARIEGSMPARVRDDVEFLRANVYLAV
                     GRSAEAAEVLRELQGTKGLTGFSEFNLAVALLQDGRTAEALQQLDRAGQVDARDAMTH
                     SIRDKANLVLGGMLLESGRFEEARAPLERVRLEGPMSNEALLGAGWAELSGENFERAV
                     VPWSILADRDPTDAAVQEAKLALPYAYSRLNVHGRAAVLYGKALDTFGRELNKLDASM
                     GSIENGSFLEALVREEIHQDKDWVVRMRSLPETPETFYLVELLASHDFQTALQNYLDL
                     EDLRKKLATWQASFDAFDSVIAARADYFEPLLPEVDQTFRELDSRFRVRVEQYELLVK
                     RMQDLLVMPRPEFLATTEEKVLSTGLTAMDEALGGVGRDGNDELRQRIRRLEGIITWT
                     LHTQYDDRLTRFYENLQDMQGSVALLKEHYQAFVRTRQAATLSYQGYDAPIKRLRGRI
                     HQASAKVDQLMARQGRMLEQVAMAELESRQRRLVNYQHQARYALADSYDRATQAQYAE
                     EP"
     gene            8027..>9632
                     /locus_tag="AC16QF_06675"
     CDS             8027..>9632
                     /locus_tag="AC16QF_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6448274.1"
                     /translation="MPVVLAAMLLLALLAGCASRPDGGKTIEQLRGVKPDMTEVAIDD
                     SLNKALASYRHFLNETPVNNKTPDAMRRLADLQIEKEYGVIGDGSGVTLAAPARLAME
                     DAVGASTEGDAAIADHSEPEAEFERRATAPLALPSANWARAAEAGLPDGLSAAATAGP
                     LEAIATYQKILDRYPYYERNDQVLYQMARAYDELGEPDKAMEVMDWFINDYRYSHYAD
                     EVYFRRAEYFFVRKQYLEAEEAYSAVVSIGSGSQFYELALYKLGWTFYKQDFYEEALH
                     RFMALLDYKQSMGYDFDRVLAEQTSDPDGAGDEEAAEDGDLDGQTGAAVAAVDVPGAD
                     ATGEAEPDEDDDRRVADTFRVVSLSFSNLGGPQVINEYYATYGNRSYEDRIYSNLGEF
                     FLSKLRYNDAAKVYRSFTELHPFHRIAPHFSMRVVEIYAKGDFGQLVVEAKKDFARRY
                     GLNAEYWQHAEIEKSPDVLSYLKTNLKDLASHYHALYQMEELEKERPGNYAEALTWYR
                     ELLASFPTDDDSAATNYQLADLQFENG"
BASE COUNT         1775 a         3243 c         3059 g         1555 t
ORIGIN      
        1 cgaaccggca accgcgtctg cgccagcagg tggccgaccg gatacgcccc gggggcttcc
       61 agcacacgcc gcttggacac gccgaacgcc aggagaacca gctcatcgct ttccaggagc
      121 cgcgccaggg tcatggagac ccgggcggac gggctggctt gggtccggac ccgcaggcag
      181 cacgcgttgc tttccagatc caacgcctcg tcgaggttgt ccgcgtccgg gaacaccgag
      241 gcgaaatggc catcctcgcc catgccgacc agggcgcacg ccaacggcgc cgagaaagca
      301 cgcagctccg cactctgcag cggcaggaac cgcgcggccg ccgccctgcc aacgagcagc
      361 cggcgccgga ccatgccttc gttgctgtcg gggtggtctg ccggcacgca tcgttcgtcc
      421 gtcaacgtga cgcgaacccg ggcccagtcc agcggatgcg cggcaagctc gccaagacac
      481 tgccgcgggg tcgtcccgcc gctgaccgcc actacggcct cggtgctcgt tgccaaccgc
      541 cgtgacagcg ccatcgcgag cacggcggcc gccgcgcggg aggccgccga acggtcagga
      601 aaggtctcga tgccaggcat cattggccgg caaaccagtg gcggccgtcc cgatccagga
      661 ggagggacga ggccgtcggg ccccaggaac ccgaaacgta agcgtcgact ttctgccgcg
      721 cctcggccca tccggcgatg atgtcgtcga tccatcgcca ggcggtctcg atctcgtcac
      781 gccgcatgaa aagggccgga ttaccccgca tgacctcgat gagcagccgc tcgtaggcgt
      841 ccggatagcg aatgccgaag gtctcctcga agctgagatc gagagacacg ggccgcagct
      901 cgaacccgcc gggcccgggc tctttggcca tgatcgcgag ccgcactccc tcgtcgggct
      961 gcagcatgat ggagagccgg ttgggcgccg cgtccccgct cacgtccccg aacagggggt
     1021 ggggcacggg cctgaactgg atgacgatct cggagtgttt ggcgggcaga cgcttgccgg
     1081 tgcgcaggta gaacggcacc cccgaccagc gccagttctc gataccaagc ttgagagcaa
     1141 cgaaagtctc ggtatcggtg gcgccaccaa gctcgtcgac gtacccgggc acggcgtgcc
     1201 ccgatatggc gcccgccgtg tactgggccc ggaccgtgga agtacggacg ttggcggcgg
     1261 tgatgggttt aagggcgcga aggaccttga gcttctcgtc gcgaaccgcg tcgtcgtcca
     1321 ggcttgccgg cggctccatg gcgaccaggc agaccagctg cagaagatgg ttctgcacca
     1381 tgtcgcgcac ggccccgatc cggtcgtaaa aggccacccg gtttcccacg ccgagctcct
     1441 cggccaccgt gatctgcacg tgatccacca cttcccgccg ccacaacggc tcgaacagcg
     1501 agttgccgaa cctcagcgcc agcaggttct gcacggtttc cttgccgaga tagtgatcga
     1561 tgcgaaagat ctggttctcg gcgaagcagg cgcctacctc gtcgttgatg gctcgcgccg
     1621 aggcgtagtc gtcgccaacc ggcttctcca gaacgatgcg catgtccgga accatcagag
     1681 agttggactg gaaaccgcgg gccacgctgc cgaaaaggtg ggggggtgtg gcgagataga
     1741 cgatgcgaat cttgccctca tgtccccgca ggcattcggc cagggggccc cagccggagt
     1801 gatcgtccgc atccaggtcg agataatcca gacgtgcggc gaacgtctcc cagatgtccg
     1861 gctctctccc ttcgccggcg gcttccacgg cgcgcgccgc ctcggagagg tattccgccc
     1921 tgcccatggc gcttcgaccc accgcgatga tgcggctccc gggcggaatc tgcccatccc
     1981 ggtggcggtg gtacaaggcc ggcaggagct tgcgcagcgc aaggtctccg gcaccaccga
     2041 agatgatcag gtcgaacgga tcgacgggga tgagctgcgc cactgagcgc tccactgaac
     2101 aagaatttcg taatttgact acattaatcc cgaggatcgc aattatttcg tggccattta
     2161 ccaaaatact atgtagtttt actacatgac tacgggaata cttcgccaga tcgatctgga
     2221 tctgccccgc ttgagccgct cggaaacccg catcgcggaa tggatccggg aaaacccgcg
     2281 gcagacggtc gacgcctcca tcggcgaagt cgccgccaac gcaggcgtga gcgaaccaac
     2341 ggtgattcga ttctgccggc ggctgggtct tgccggcttc cgggatctca agacacggct
     2401 ggtcgccgcg ctgcagagcc cggagaactt cctgcatcgc gacgtcgacg acgcggacgg
     2461 gccttccgag gccgccgcca aggtgctcga cgggtccatt cgaaccctgg tcgaccttcg
     2521 cgagcagacg ccgaccatgc cgttccgtgc cgccgtggaa accatgacga cggcccggga
     2581 actcgtgttc gtcgggcttg gcgcctcggg gcacgtcgcc agggatgcaa gccacaaatt
     2641 ctttcgcctg ggcattccct gcagcacggc ggtggatgcg caaacgatcc tgcagcgggc
     2701 cgccatcgcg tcggcccgcg acgtcttcgt ggtcgtctcc cacaccggca cctggcccga
     2761 aatcgtcacg gccatggcca tcgccgccga acgtcacgcc cggatcgtcg ctctgaccga
     2821 tccccgctcc cccctggcga cgactgccga cctcgtcttc gcctgccatc cggtggagga
     2881 caccaacgtg tttacaccca tgagctcccg gctcgcccag ctcgcgctcc tggacgcgct
     2941 gcaggttgcg ctcgcgctgg gcatcggcga gccggccaag cagaacctgc gggcgagcaa
     3001 ggaggcgctc tccgagatgc gcgccagcgc gtgcggatcc aactgaggtg agcgtggatc
     3061 ggatcagcgg gcgacggatt tactgtcgcg ttctgaccgg gggcgagctc ggcgcgggca
     3121 agggcatcaa caaacggggc ggcggcctct ccgccctggc cgacaaccca cgcacgctcg
     3181 cccgggtagc gctgttccgt ggcgtgcatc ccgaactctt cgagaccgag catctggact
     3241 acgagctcat caacgaagcc gccgtcacct ggctcgcgca caagggcgcc gtaagcaggg
     3301 gggatcgagt cattctctcc aaaggcgact accgtaacgt gcagggcggc accaacacac
     3361 tgaaaatact ggaggtcgcc tgatgcgaaa gctgcgcgta ggcatcatgg gtttcggccg
     3421 ggtcggtcgg cagatttacc ggctcgccct caaggacgag cgtttcgagg tggttgccat
     3481 atccgacatc gggcgcccgg aaatcctgca ccatctgctg accaggaccg atgacgacgt
     3541 gggcgtgacg ctaagcggca actacctcga atccaggggc gttcgcaccc gcctgatgcc
     3601 ggccgatcat cccacggaga ttccctggga cgtcttcggg gtcgacgtgg tcatcgaggc
     3661 gaccggcagg tttcatcgcg ccgacgatct gcggcctcac ctgcacaacg gcgctccgcg
     3721 cgtcgtcctg tcggcacttc cccaggatga catcgatcgc gtggtgctcg tcggcgtgaa
     3781 cgactgcgag gcaagcgcgg ccgacgtcat ggtgtccgcc gggtcggctt ccaccaccgc
     3841 aaccgcgctg gcgtgcaaga cgatcaccga agtctggccc gtggaacatg ccaccatgac
     3901 gtccgtgcac gcctacacca gcgatcagag cctgcaggac tacgccggac cggactatcg
     3961 gcgcagccgc tccggagcgg aaaacatcat ccccaacgcg accccggccc tgaagtgggt
     4021 tcagcgggcc gtgccggcga tggcaggcag aatgaccggg tacgcgctca acgtgcccgt
     4081 ccaggtcggc tcgatgctcg acctcacctt ctcgttccgg gaatccggca tcggcgccga
     4141 cgacgtgaac gacctgttcg gcaacgctgc acgggtacgg ccggatctga tcgccaccac
     4201 ccgggacccc atcgtttcat ccgacgtcag gggctcctcc gagtctctcc tcgtcgacat
     4261 ggagggaacc atggcggccg gcagtcgcat ggtgaaggtg ctcgcctggc acgaaaccct
     4321 cgggcatgcc cggcgcatcc tggacgtcgt cggcctctac gccgacctgg acgacgcaac
     4381 agcaaaggag gcttcctgat gcgagtcgcc atcaacggct tcggccgcat cggccgctcg
     4441 gtcttccgca tcgcggaaac gctgggggac atcgagatcg tcgccatcaa cgacctcttc
     4501 gacgacgagg cgcttcgcta cctgctcacc tacgacaccg tgatgggtcg attcgaaggc
     4561 gacgtgcgca tcgaggacgg gcacatgatc acgcccaaga gccgggcacg aatgctcgcg
     4621 cttgcagatc ccacacagct gccatgggcg gagctcgaga tcgacgcggt ggtggagtcc
     4681 acgggcgtgt tccgcagccg cgagcaggtt tcccagcatc tcgaggccgg tgccgggcgc
     4741 gcgctgctga ccgtgccggc aaaggacgat atcgactaca ccgtggtgct gggggtcaac
     4801 ggggaaggct tgaaggccga gcaccgggtc gtttccaacg ccagctgcac caccaactgc
     4861 ctcgcacctc tggcgaaggt cctgcacgac gccttcggca tccgcgaagg cgtcatcaac
     4921 accgtgcacg cctacaccaa cgaccagcgg ctggccgacg tgccccactc cgactggcgc
     4981 cgcagccggg ccgcagccga gaacatcatc ccgacctcca ccggcgcagc caaggcggtg
     5041 ggcgtcgtcc tgccggagct tgcaggcagg ctcgacggca tcgcttcccg cgttcccgtc
     5101 ccggacggct cggtggtgga tcttttcgtg gagctcgaga agaacgtgag cgtggacgac
     5161 gttcacgagg tgctggaggc tgccgccggt tccgagccga tgagcggcgt gctggagttc
     5221 acgagggagc ccatcgtctc ttcggacatc atcggcaacc cgcactcgtc gatctacgat
     5281 gcgtcgttta ctcaggtggc cggggggcgg ttcttaaagg cgctttcctg gtacgacaac
     5341 gagtggggtt actcgaaccg ggtgtgcgac gtcctgcgga tgatgtccgc cctggactga
     5401 cccgcgtcct cgaaaatctg agacgtctac acccttcaaa cgctgaatag cgcaagctta
     5461 cgggcccgtt cccgggcccg cggcctgctt tgagcgctca gtcacacacc gcgcggcacg
     5521 attcgtcaag acttgcgccg gaacagggac aaacgcgggt ggactgtgga gacgcgagag
     5581 gcattccacg caccgcgtca ttaggtgttc gagatgccaa agaccccaat aatcgcattg
     5641 ctgactgcgc tggcgtgcct gtgctggtcg ccggtttcgg ccgacgacgt ttcccgcaag
     5701 gagatgcgca gcctcgacga gcaggttcag gaaatcaaat ccgacgtgct gagcatcgcc
     5761 gcggaactcg accagctcga ggagaggctg ctctttccgt ccaacaccca gctgaccgtc
     5821 ttcgtcgcca tcggcgacgg gacggagtct ttccggctcg actcgatccg gctcggcatc
     5881 aacgatgcgc tcgccactca ccacatctac agcttcaagg aactcgaggc gctgcagcag
     5941 ggaggcgttc agcgtctcta catgggcaac ctgcccatcg gcactcacgc gctggaggtt
     6001 tccgtgctgg gcaagctgcc gagcggcaag gactttgccc acgccgagcg cttcaccttc
     6061 gacaagggca tagacccggc tctgatcggc atcaccgttg acccgggctc cggcaaggcc
     6121 ccgatcgccc tgaaagactg gtagccatga ccagacccct cgcctggacc atcgggttgc
     6181 agctggcctt gatgctgctt tgcagcccgg cgcgcgccga tgacgtccgc gacctgtatt
     6241 ttggcgaggc gttgttccag gccggtcaag gcaagtactt cgaagcgctg gaacgactcg
     6301 acgcggaact cggccagcac tacggtctcg acgagccgga actggacacc ctgcaccacc
     6361 acatcaggga agccgagttc ttcgtcggcg acttcgagct caactaccgc atgcatcatc
     6421 gcgcgggccg cgccatcagc gcggtcttga acggcgacgt ggatcagtcc gtgcgcaacg
     6481 aagccgccta caggctggca cgcattcact ttcagaaagc ccagatggcc gatgccctgg
     6541 aagcgcttgc gcgcatcgag ggatccatgc ctgcgcgcgt tcgagacgac gtggagtttc
     6601 tccgcgccaa cgtctacctc gccgtcggcc gctcggccga agccgccgag gtgctccggg
     6661 agctgcaggg aacgaagggg ctcaccggct tttccgagtt caacctggcg gttgctctgc
     6721 ttcaggacgg ccgcacggcc gaggccctgc agcagctcga ccgggcaggt caggtcgacg
     6781 ccagggacgc catgacccac tccattcgag acaaggcgaa tctggtgctc ggcggcatgc
     6841 tgctggagtc cgggcggttc gaggaggccc gggcgcctct ggagcgcgtt cgcctggaag
     6901 ggcccatgtc caacgaggcg ctcttagggg ccggctgggc cgagctatcc ggcgagaact
     6961 tcgagcgcgc tgtggtgccc tggtccattc tggccgatcg cgacccgacg gacgcggccg
     7021 tgcaggaagc gaagctggca ctgccatatg cctacagccg cctgaacgtt cacggacgag
     7081 cggccgttct ctacgggaag gcgctcgaca ccttcggccg ggagctcaac aagctcgacg
     7141 cctccatggg tagcatcgag aacgggtcgt tcctcgaggc gctcgtgcgc gaggagatcc
     7201 accaggacaa ggattgggtg gttcgcatgc gcagcctgcc cgagaccccc gagaccttct
     7261 acctggtaga gctgctcgcc tcccacgatt tccagaccgc tctgcagaac tacctggatc
     7321 tggaggacct gcgaaagaaa ctcgccacct ggcaggcgag cttcgacgcc ttcgacagcg
     7381 tcatagccgc ccgggccgac tacttcgagc ccctgctccc cgaggtcgac cagacgttcc
     7441 gcgagctcga ctcccgcttt cgcgttcgcg tggagcagta cgagctgctg gtgaaacgca
     7501 tgcaggacct cctggtcatg ccccgtcccg agtttctggc caccaccgag gagaaggtcc
     7561 tgagcacggg tctgaccgcc atggacgagg ctcttggcgg ggtgggccgc gacgggaacg
     7621 acgagctacg tcagcgcatc cgccgtctgg aagggatcat cacctggacg ctgcacacgc
     7681 agtacgacga tcggctgacc cgcttctacg agaatctcca ggacatgcag ggatccgtcg
     7741 ccctgctcaa agaacactac caggcgttcg tccgcacccg ccaggccgca acgctcagct
     7801 accagggata cgacgcgccc atcaagcgcc ttcgcgggcg gattcatcag gcttccgcaa
     7861 aggtggacca gctcatggcc cgtcagggcc ggatgctcga gcaggttgcc atggcggagc
     7921 tggagtcacg gcagcggcgc ctggtgaact accagcacca ggcacgatac gccctggcag
     7981 acagctatga ccgcgccacc caggcgcagt acgcggagga gccgtgatgc ccgtcgttct
     8041 ggccgccatg ctgctccttg cgctgctcgc cggttgcgcg agccgccccg atggcggaaa
     8101 gaccatcgag cagctgcgcg gcgtgaagcc cgacatgacc gaggtggcca tcgacgacag
     8161 cctcaacaag gcgctggcca gctaccggca ctttctcaac gagacgcccg tcaacaacaa
     8221 gacgccggac gcgatgcggc gccttgcgga tctgcagatc gagaaagaat acggcgtcat
     8281 cggcgacggc agcggcgtga cgctcgccgc acccgcgcgt ctggcgatgg aggatgcggt
     8341 gggcgccagc accgaaggtg atgccgccat cgccgatcac tccgagccgg aagcggaatt
     8401 cgagcgccgc gcgaccgcgc cgttggcgct gccgtccgca aactgggcgc gtgcggccga
     8461 ggctggtctg cccgacgggc tgtctgcagc agccaccgca ggcccgctgg aagcaatcgc
     8521 cacctatcag aagatcctcg accgctaccc gtactacgag cgcaacgatc aggttctcta
     8581 ccagatggcg cgggcctacg acgagcttgg cgagccggac aaggccatgg aggtcatgga
     8641 ctggttcatc aacgactacc gctactctca ctacgctgac gaggtgtact tccgccgcgc
     8701 cgagtacttc ttcgtgagga agcagtatct ggaggccgaa gaggcctact cggccgtggt
     8761 ctccatcggt tccggctcgc agttctacga gctggctttg tacaagctgg gctggacgtt
     8821 ctacaagcag gacttctacg aggaagccct gcaccggttc atggcgctcc tggactacaa
     8881 gcagtccatg ggctacgact tcgaccgggt gctggccgag cagacatcgg atcccgacgg
     8941 ggccggcgat gaagaggccg ccgaggatgg cgatctcgac gggcagacgg gcgccgcggt
     9001 cgcggccgtc gacgtgccgg gcgccgacgc caccggggaa gccgagcccg acgaggacga
     9061 tgaccgccgc gtggcggaca ccttccgcgt ggtgagcctg tccttctcca acctgggcgg
     9121 accgcaagtc atcaacgagt attacgccac ctacggcaac cgcagctacg aagaccggat
     9181 ctacagcaac ctcggcgagt tcttcctgag caagctgcgc tacaacgacg cggccaaggt
     9241 ctatcgttcc ttcactgagc tgcatccctt ccacaggatc gcgccccatt tcagcatgcg
     9301 cgtggtggag atttacgcca agggcgattt cggccagctg gtagtggaag ccaagaaaga
     9361 cttcgcccgt cgctacggcc tcaacgccga gtactggcag cacgcggaga tcgagaagtc
     9421 gcccgacgtc ctgagctacc tgaagacgaa cctcaaggac ctcgccagcc actatcacgc
     9481 cctctaccag atggaagagc tcgaaaagga gcggcccggg aactacgccg aggcgctcac
     9541 ctggtaccgg gagctgctgg catccttccc caccgacgac gactccgcgg ccaccaacta
     9601 ccagcttgcc gatctgcagt tcgaaaacgg cg
//
LOCUS       DBNHLM010000143         6734 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_319547, whole genome shotgun sequence.
ACCESSION   DBNHLM010000143 DBNHLM010000000
VERSION     DBNHLM010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6734)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6734
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_319547"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="AC16QF_06680"
     CDS             complement(<1..242)
                     /locus_tag="AC16QF_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR6448275.1"
                     /translation="MTRGLLLVNLGTPLSSDVRDVRAFLGEFLMDRYVLDVPWLLRRL
                     IVTGFILPFRPNQTARAYAKIWNAAGPETGSPLLHYS"
     gene            335..958
                     /gene="hisH"
                     /locus_tag="AC16QF_06685"
     CDS             335..958
                     /gene="hisH"
                     /locus_tag="AC16QF_06685"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR6448276.1"
                     /translation="MSHAIIIDYDAGNLRSVQRACSEVGIDAEISADADRVAAADRLI
                     FPGVGAAGSAMRSVRRNGLDQALRSALAGGTPVLGICLGLQISFDHSEEDDQDTLGLL
                     PGLVKRFAFERRDLKIPHMGWNEVRVVRPHPLLDDIEPGDEFYFVHSYYPVPAEPELV
                     FAVTDYERDFACAVGRDNYFATQFHPEKSGRVGLRLLARFAAWDGRC"
     gene            952..1731
                     /gene="hisF"
                     /locus_tag="AC16QF_06690"
     CDS             952..1731
                     /gene="hisF"
                     /locus_tag="AC16QF_06690"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR6448277.1"
                     /translation="MLSKRLIACLDVRDGKLAKSVKFVDTKDIGDPVEKAREYYLDGL
                     DELVFYDITASSDKRRIMLDVVEAVAEQVFIPLSVGGGVSSVGDATDLRLAGAEKINV
                     NSAAVRRPQLIAECAEAIGRQNVVLSMDIRRVEPCAELPSGYEIVIEGGRRPMAIDAL
                     EWALEGERLGAGELVINSIDADGTRDGYELTLTAMLAEAVRIPVIASGGAGKPEHLYD
                     ALTVGKADAALVASMVHYGDYKVSELKRYLDGRGVKMRMSW"
     gene            1780..2742
                     /locus_tag="AC16QF_06695"
     CDS             1780..2742
                     /locus_tag="AC16QF_06695"
                     /EC_number="1.6.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017255625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HIR6448278.1"
                     /translation="MKAVIIEGERLSWQEAEEPQAGVGEVLIAVHATAVNRADLVQRS
                     GGYPPPPGASKILGLECAGEVLAIGEGVTRVKPGDRVCALLAGGGYAERVAVPAGQVL
                     PVPSGLDMSQAAALPEVFATAYLNLYTEARLERGERVLIHAGASGVGTAAIQLCSTTS
                     NPCFVTVGNPQKIERCVALGAEGGWDRHQGSFLEAVRQWGGVDVVLDPVGAGYLADNL
                     SCLNIDGRLSLIGLMGGVQSEINLATVVMKRIRIIGSTLRARSAAAKSLVMDGLQRDV
                     WPHLEAGSIVPIIDEVLPIESAERAHELMAGDQTFGKLVLQIVE"
     gene            complement(2725..5499)
                     /locus_tag="AC16QF_06700"
     CDS             complement(2725..5499)
                     /locus_tag="AC16QF_06700"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR6448279.1"
                     /translation="MESSFDPHAIEQKLYADWEGADYFAPRGNGEGYCIAIPPPNVTG
                     SLHMGHAFQHTLMDALIRYERMKGRRTLWQMGTDHAGIATQMLVERQLLAEGLKREDI
                     GRDAFVERVWDWRNHSGNNISKQLRRMGSSLDWSRDRFTMDEGFCHAVLEVFVRLYEE
                     GLIYRGQRLVNWDPELKSVISDLEVENTEERGHLWHFRYPLAGGLTTREGKPYLVVAT
                     TRPETMLGDTAVAVHPDDERYAALVGGSVILPLADREIPIIADEYVDRDFGTGCVKIT
                     PAHDFNDNEVGARHGLALINVFNEDATINEAAPQPYRGLDRFDAREKVVADLKSQGLL
                     DGIEDHTLMVPRGDRSNAIVEPLLTHQWFVKIQPLADPAIKAVENGDVEFVPKQYENV
                     YFSWMRNIQDWAISRQQWWGHRIPAWYDAEGNVYVGRDEDEARRNGGLADDVPLDQDP
                     DVLDTWFSSALWTFVTLGWPEETEELSQFHPTDVLVTGHDIIFFWVARMIMMTLKFTG
                     EVPFRQVYVHGLVRDADGKKMSKTKGNGLDPLDLIDGVSLDELIDKRTQNLTQPQMAP
                     SIEKNTRADFPEGIPSYGTDALRFTYCALASTGRDVRFDLSRIEGYRNFCNKLWNAAR
                     FVLMNCPDDEEPGRRDLSLADRWIVSEMRSFLETSERAINTYRFDLYANAVYEFVWHE
                     YCDWYLELTKPLLWDEQADPSLLRGTRYTLLSVLDTLLRAAHPIMPFITETIWREVAP
                     RLGNTNPTIMLQPFPEPADLPSDPDAEAAVEWLKGVIVGIRNIRGEAGIKPAQEISIL
                     LQGGSDADRALAQVAESLLKRLAKVDRIDWLADGAEPPPNALALVGELRVMVPLAGLI
                     DVAAERARLAKEVEKKSQELNRIEAKLANENFVAKAPQAVVEKERAKAGDARAALATL
                     EQQLASLDDL"
     gene            complement(5489..5974)
                     /locus_tag="AC16QF_06705"
     CDS             complement(5489..5974)
                     /locus_tag="AC16QF_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448280.1"
                     /translation="MSTKDSADSEDELLGDLESLRSILDEEDELMPQDDEAVPLLDDV
                     VADAADAEEPARASPGGLDDDLFQALLSDDWRESAAAIIEQAPAALGQAVAWKPREIA
                     ALRQALRLKQDETLNQWISDALERHAEALQAELLEAFSGELKPAVEGILRRDAGGEHG
                     E"
     gene            complement(5974..6411)
                     /locus_tag="AC16QF_06710"
     CDS             complement(5974..6411)
                     /locus_tag="AC16QF_06710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HIR6448281.1"
                     /translation="MTRIDFYILQDVDLDAMRRFACRLAGKAITGGHEVHIHTQSAED
                     ASIVDELLWSYPDDRFIPHETLDDSGRAAVAAPVTLGWTEPDGLDGVLINLSDEIPRF
                     FGRFDRVAEVIPRSRRDAGRDRYKFYRDRGYPLFHHELDDWEA"
     gene            complement(6416..>6734)
                     /locus_tag="AC16QF_06715"
     CDS             complement(6416..>6734)
                     /locus_tag="AC16QF_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR6448282.1"
                     /translation="TGLYANQDRLAEQLLAAGVESHDRAWRMPLWDEYQAQLKSRFAD
                     VANIGGPQAGSVTAACFLSRFTDSMRWAHMDVAGSAFQGGANKGATGRPVGLLFRFLI
                     GQS"
BASE COUNT         1088 a         2111 c         2291 g         1244 t
ORIGIN      
        1 gagtaatgca gaaggggcga gccggtttcc ggtcctgccg cattccagat cttggcgtag
       61 gcccgggcgg tctggttggg acggaacggc agaatgaacc cggttacgat caggcgccgc
      121 agtagccagg gcacgtcgag gacgtatcgg tccatcagaa attcgcccag gaacgcgcgc
      181 acgtcgcgaa cgtcactcga aagcggggtg ccgaggttga ccagcagcag gcctcgggtc
      241 acgggaggtc tccggtgaat gtagtttgcc caagcgcgat gggatagtat ccgcgctcgt
      301 tttggatgtc acttttccta ccggatccca cgggatgtcc cacgccatca tcatcgacta
      361 tgacgccggc aacctgcgca gtgttcagcg ggcctgctcg gaggtcggca tcgatgcgga
      421 gatcagcgcc gatgccgacc gcgtggcggc cgccgaccgg ctgatcttcc cgggcgtcgg
      481 cgcggcgggg agcgcgatgc gaagcgtgcg ccgcaacggg ctggatcagg cgttgcgctc
      541 ggccctggcg ggcgggaccc ccgttctcgg catctgcctg gggctgcaga tatccttcga
      601 ccactccgag gaagacgatc aggacacgct gggcctcctt cccgggctgg tcaaacgctt
      661 cgcgttcgag cggcgtgatc tgaagatccc gcacatgggc tggaacgagg tccgggtggt
      721 gcgcccgcac ccgctgctcg acgacatcga gcccggcgac gagttctact tcgtgcattc
      781 ctattacccg gtgcccgcgg aaccggagct cgtctttgcg gtgaccgact acgagcgcga
      841 ctttgcctgc gcggtgggca gagacaacta cttcgccacc cagtttcatc cggagaaaag
      901 cggccgggtc gggctccggc tgctcgcgcg ctttgctgcc tgggacggcc gatgctgagc
      961 aagcgtctga tagcctgtct cgacgtccgc gacggcaagc tcgcaaagag cgtgaagttc
     1021 gtcgacacga aagacatcgg agatccggtc gaaaaggccc gcgagtacta cctggacggg
     1081 ctcgacgagc tggtcttcta cgacatcacc gcatcgagcg acaagcgtcg catcatgctc
     1141 gacgtcgtcg aggccgtggc cgagcaggtt ttcattccgc tctctgttgg cggcggggtc
     1201 agctcggtgg gggatgccac ggatctgcgc ctggccgggg cggaaaaaat caacgtcaac
     1261 tcggcggccg tgcggcgccc gcagctcatc gccgagtgcg cggaggccat cgggcgtcag
     1321 aacgtggtct tgtccatgga catccgacgt gtggaaccct gcgcggagct gccttccggt
     1381 tacgagatcg tgatagaggg cggccgtcgg cccatggcca tagacgctct ggagtgggcc
     1441 ctggaagggg agcggctcgg cgcgggcgag ctggtgatca actccatcga tgccgacggg
     1501 acccgggacg gctacgagct caccctcact gccatgctgg cggaggccgt gcgaattcct
     1561 gttatcgcct cgggtggtgc gggcaagccc gagcaccttt acgatgcgct gaccgtgggg
     1621 aaagcggacg ctgcgctggt ggcttccatg gtgcactacg gcgattacaa ggtgagcgag
     1681 ctgaagcggt acctcgacgg ccgcggggtc aagatgcgga tgtcctggtg agcatcagcg
     1741 tgggcgagcg aaaaaggcca ttaccagtca gggagagcaa tgaaagccgt catcattgaa
     1801 ggagagcgtc tgagctggca ggaggccgaa gagcctcagg ccggggtcgg tgaggtgctg
     1861 atcgccgtgc acgcgacggc cgtcaaccgt gcggatctgg tgcagcgctc gggaggctac
     1921 ccgcccccac cgggagcgag caagatcctg ggcctggaat gcgcgggaga ggtgctggcc
     1981 attggggaag gcgtgacccg ggtaaagccc ggcgatcggg tctgcgcgct cctggccggg
     2041 ggcggctacg cggagcgcgt cgcggtccct gcggggcagg tcctgccggt gccgtccggg
     2101 ctggacatgt cgcaggcggc cgccctgccg gaggtatttg ccaccgctta tctcaacctt
     2161 tacacggagg cgcgtctgga gcgcggtgag cgcgtgctca tccatgccgg ggcaagcggc
     2221 gtcggtaccg cggccattca gctgtgcagc accacgagca atccctgctt cgtaaccgtg
     2281 ggcaatcctc agaagatcga acgctgcgtg gctttggggg ccgagggcgg ctgggatcgg
     2341 caccagggca gtttcctcga agccgtcagg cagtggggcg gcgtcgacgt cgtgctcgat
     2401 ccggtcggag ccggctacct tgcagacaac ctttcctgtc tcaacataga tggccggctc
     2461 tcgctgatcg gtttgatggg tggggttcag tccgagatca acctcgccac cgtggtcatg
     2521 aagcgaattc gcatcatcgg ctccacgctc agggcgcgct cggcggcggc caagtcgctg
     2581 gtcatggacg gtctgcagcg ggacgtctgg ccgcatcttg aagcggggtc catcgttccg
     2641 ataatcgacg aggtgctccc catcgaatcc gcggagcggg ctcacgagct catggcgggc
     2701 gatcagacgt tcggtaagct ggtgttacag atcgtcgagt gaggcgagct gctgctcgag
     2761 cgtggccagc gcggcgcgtg catcgccggc tttggcccgc tccttttcca ccaccgcctg
     2821 cggggccttg gcgacgaaat tctcgttggc cagcttcgcc tcgatgcggt tgagctcctg
     2881 ggactttttc tccacttcct tggcgagccg cgcgcgttcc gcagccacgt cgatcaggcc
     2941 ggccaggggg accatcactc tcagctcgcc gacgagcgcc agagcgtttg gtggcggttc
     3001 cgcgccgtcg gccagccagt ctatgcggtc gacctttgcc agccgcttga gcagcgattc
     3061 cgccacctga gcgagcgccc gatccgcgtc gctgccgccc tgcagaagaa tgctgatctc
     3121 ctgcgctggc ttgatgccgg cttctccgcg gatgttgcgg atgccgacga tgacgccctt
     3181 gagccactcg acggccgctt ccgcgtccgg atccgacggc aggtccgcag gctccggaaa
     3241 cggttgcagc atgatggtcg ggttggtgtt gcccagccgc ggggccacct cgcgccagat
     3301 ggtctcggtg atgaacggca tgatcgggtg ggctgcgcgc agcagcgtgt ccagcacgct
     3361 cagcagcgtg tagcgcgtcc cgcgcaggag gctcgggtcg gcctgctcgt cccacaacag
     3421 cggcttcgtc agctccagat accagtcgca gtattcgtgc cagacgaact cgtacacggc
     3481 gttggcatag aggtcgaagc ggtaggtgtt gatggcgcgt tcggacgtct ccaggaaaga
     3541 gcgcatctcg gagacgatcc agcggtcggc caggctcaga tcgcgcctgc ccggctcttc
     3601 gtcgtcgggg cagttcatga gcacgaaacg ggccgcgttc cagagcttgt tgcagaagtt
     3661 gcggtatccc tcgatgcggc tgaggtcgaa acgcacgtcg cggccggtgc ttgccagggc
     3721 gcagtaggtg aaacgcaggg cgtccgttcc gtaggacgga atgccttcag ggaaatcggc
     3781 ccgggtgttc ttttcgatac tcggggccat ctgcggttgg gtgagattct gggttcgctt
     3841 gtcgatcagc tcgtccagag agaccccgtc gatcaggtct aggggatcca ggccgttgcc
     3901 cttggtcttg gacatcttct ttccatcggc gtcgcgcacc agcccgtgca cgtagacctg
     3961 ccggaacggc acctcgccgg tgaacttcag cgtcatcatg atcatcctgg ccacccagaa
     4021 gaagatgatg tcgtgacccg tgacgagcac gtccgtgggg tgaaactgag aaagctcctc
     4081 ggtctcctcc ggccagccca gcgtcacgaa cgtccacagc gcggaggaga accaggtgtc
     4141 caggacgtcc gggtcctggt ccaggggaac gtcgtcggcg aggccgccgt tgcggcgggc
     4201 ttcgtcttcg tcccggccca cgtagacgtt gccttcggcg tcgtaccatg cggggatcct
     4261 gtggccccac cactgctggc gcgagatggc ccagtcctgg atgttgcgca tccaggagaa
     4321 gtagacgttc tcgtactgct ttggaacgaa ctcgacgtca ccgttctcga cagccttgat
     4381 ggccgggtct gccagcggct ggatcttcac gaaccactgg tgagtcagca gcggctcgac
     4441 gatcgcgttc gagcggtctc cccggggaac catcagcgta tggtcctcga tcccgtccag
     4501 gagcccctgg cttttcaggt ccgccaccac tttctcgcga gcgtcgaacc ggtccagccc
     4561 gcggtagggc tggggcgcgg cctcgtttat ggtggcgtcc tcgttgaaga cgttgatcag
     4621 cgcgagcccg tgtcgtgcgc ccacctcgtt gtcgttgaaa tcgtgggccg gcgtgatctt
     4681 cacgcagccg gtgccgaagt cgcgatcgac gtactcgtcg gcgatgatgg gaatttcccg
     4741 gtccgccagc ggcagaatca ccgagccgcc caccagagcg gcgtagcgct cgtcgtcggg
     4801 gtgcacggcg acggccgtgt caccgagcat ggtttcgggt cgggtagttg caaccaccag
     4861 gtacggcttg ccctcgcggg ttgtcaggcc gccggcgagc ggatagcgga agtgccagag
     4921 gtgcccgcgc tcctcggtgt tctcgacttc cagatccgaa atgaccgatt tcagctccgg
     4981 gtcccagttc accaggcgct ggccccggta gatgagccct tcctcgtaga ggcgcacgaa
     5041 cacctcgagc acggcgtggc agaagccctc gtccatggtg aaccggtcgc gggaccagtc
     5101 gagagacgaa cccatgcggc gcagctgctt ggagatgttg ttgccggagt ggttgcgcca
     5161 gtcccagact cgctcgacga aggcgtcccg gccgatgtcc tcccgtttca gcccctctgc
     5221 cagcagctgc cgctcgacca gcatctgggt ggcgattccc gcatgatccg tacccatctg
     5281 ccacagcgtg cgtcgcccct tcatgcgctc atagcggatg agcgcgtcca tcagggtgtg
     5341 ctggaacgcg tgccccatgt ggaggctgcc cgtgacgttg ggtgggggga tggcgatgca
     5401 gtagccttcg ccattgcccc ggggcgcaaa atagtccgcg ccttcccaat cggcatacag
     5461 tttctgctcg atggcgtggg gatcgaaact actctccatg ttcgcccccc gcgtcgcgac
     5521 gcaagatgcc ttctaccgcg ggcttcagct caccggagaa ggcttcgagc agctcggctt
     5581 gcagcgcttc ggcgtggcgc tcgagtgcat cacttatcca ctgattcagg gtttcgtcct
     5641 gcttcagacg cagggcttgc cgaagggccg cgatttcccg gggcttccag gcaacggcct
     5701 ggccgagggc agcgggcgcc tgctcgatga tggcggcggc cgattcgcgc cagtcatcgc
     5761 tcagcagcgc ctggaacagg tcgtcgtcca atcctcccgg actggcccgg gcgggctcct
     5821 ccgcgtcggc cgcatctgcg accacgtcgt ccagaagcgg aacggcctcg tcgtcctggg
     5881 gcatcagctc gtcctcctcg tccagaatcg aacgcaggga ttcgaggtcg cccagcagct
     5941 cgtcctcgga gtctgcagag tctttggtgc tcatcacgct tcccagtcgt cgagctcgtg
     6001 gtggaacagc ggatatccgc ggtcccgata gaacttgtat ctatcccgtc ctgcgtcccg
     6061 gcgtgaccgc gggatcacct cggcgacccg gtcgaagcgg ccgaagaagc gcgggatctc
     6121 gtcgctcagg ttgatgagca cgccgtccag accgtcgggc tcggtccacc ccagagtcac
     6181 cggcgcggcg acggccgcgc ggccggagtc gtcgagggtc tcgtggggga tgaagcggtc
     6241 gtcggggtac gaccagagca gctcgtccac gatgctggca tcctcggcgc tctgggtatg
     6301 tatgtggacc tcgtggccgc ccgtgatggc cttgccggca agacggcagg cgaatctgcg
     6361 catggcgtcc agatcgacgt cctggaggat gtagaagtcg atgcgggtca tggggtcaag
     6421 actgcccgat gaggaagcgg aacagaagac ccacaggccg gccggtggct cccttgttcg
     6481 ctccgccctg gaatgcgctg ccggccacgt ccatgtgtgc ccagcgcatg gagtccgtga
     6541 accgggatag gaaacaggcc gcggtgacgg agcctgcctg ggggcctccg atgttggcga
     6601 catcggcgaa gcggcttttc agctgggcct ggtactcgtc ccacaggggc atgcgccagg
     6661 cccggtcgtg gctttcgacg cccgcggcca gcaattgctc cgccagtctg tcctggtttg
     6721 cgtagagccc ggtg
//
LOCUS       DBNHLM010000144         4235 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1157266, whole genome shotgun sequence.
ACCESSION   DBNHLM010000144 DBNHLM010000000
VERSION     DBNHLM010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1157266"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /gene="paaA"
                     /locus_tag="AC16QF_06720"
     CDS             complement(<1..607)
                     /gene="paaA"
                     /locus_tag="AC16QF_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014872579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HIR6448283.1"
                     /translation="MYTQGLDSLGDESLHIPSDELAELEARFQARIDADEKIEPKDWM
                     PHAYRMTLVRQISQHAHSEVVGMLPEGNWISRAPTLRRKSVLIAKVQDEGGHGAYLYG
                     AAETLGISREQMIEQLLDGQARYSSIFNYPTPTWADIGAIGWLVDGAAIMNQIPLCRC
                     SYGPYSRAMVRVCKEESFHQRQGYEIMTVMARGTDEQRQMAQ"
     gene            complement(685..1404)
                     /locus_tag="AC16QF_06725"
     CDS             complement(685..1404)
                     /locus_tag="AC16QF_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6448284.1"
                     /translation="MATFERGGVSIHYEERGSGFPILLIAPGGMRSTIGAWANAPWNP
                     IEHLEPHFKVIVMDQRNAGHSRAPIRSTDGWQVYTEDQIALLDHLGVDQFHVAGMCIG
                     GPYCMGLVRAVPDRVAAAVLFQPIGLDDNRQAFFDMFDSWAEELKPDRDDVDPEAWEA
                     FKRSMYAGDFLFNVSREFVAGCSTPLLVLCGRDLYHPESTSREIVALAPNAELIERWK
                     EPENQEAARARVLEFLVAHTP"
     gene            complement(1409..1708)
                     /gene="hmuQ"
                     /locus_tag="AC16QF_06730"
     CDS             complement(1409..1708)
                     /gene="hmuQ"
                     /locus_tag="AC16QF_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-degrading protein HmuQ"
                     /protein_id="HIR6448285.1"
                     /translation="MNRFQIAKGFEEGFEKVWRERESFLSDVPGFKHFALLRGPENED
                     HTLYASHSVWESREAFEAWTESDNFRRAHAQASAPKGTYLGHPNLETFQAVLEEG"
     gene            complement(1701..2309)
                     /locus_tag="AC16QF_06735"
     CDS             complement(1701..2309)
                     /locus_tag="AC16QF_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR6448286.1"
                     /translation="MTWLLVSNLLLLAVVGALALVVLSLARQIGILHERTAPLGLVSK
                     SQGVEEGGRVPDLELPTLTAAHVGLKDAADAGRWSALLFIAAECPICRSVLPAYLDML
                     RDREPWLEGYWVSDGSDMPAYPAYAQANGIDLERYLISQELALHLQVQQLPSIAIIDD
                     QGRLAAGRVVQSARQLHELFQRIDADRHRQPEQEGTYVHSHE"
     gene            2353..4047
                     /locus_tag="AC16QF_06740"
     CDS             2353..4047
                     /locus_tag="AC16QF_06740"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR6448287.1"
                     /translation="MEIDLWRPLAGLSLFLFSMSIIERALEDVSGRAFRRFLRRNTDH
                     PIRGIGSGTLATAILQSSSLVGVMMLALVGAGVIQMRNALSVIFGANLGTTMTGWIVA
                     TIGFKLDLDALALPLIAAGGLAAMGIRDHRASQIARVVFGVGLLLMGLDLMKGAVGNI
                     SDAIDVAVLGELGAVEYLLFGLVFTAIVQSSSATMMVTLSALHGGIIDLPAAAAVVIG
                     ADLGTTGTVLLAALRGSAAKKRVAMGHFLFNLITDLTAFALRTPLLAVILMIGLSDLL
                     ALVAFHSAFNLIGILMFLPLIGRFAGWLEGRFTDTETSVSRHLADVAVDLPEAAVEAI
                     RLETNHLMQRVICQNLKVPDPEISLPAGSLPVGVHQAGAIAAALEPEFEAAYAASKRL
                     EGEIITFAADAQAASLEKSESDLITRCQFAAREAVHASKSLKDARGDLRAFALSHRPI
                     LNGYGNRFREIMSEFYARLFLLRHGPTAERADGGPELEDLIVLSQLAHQRHEELHTAI
                     YEDVRADRLDEDEISSLLNANRELFNSNRALLSAIATITLPPEQAATFDLLPTSVV"
     gene            complement(4044..>4235)
                     /locus_tag="AC16QF_06745"
     CDS             complement(4044..>4235)
                     /locus_tag="AC16QF_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615080.1"
                     /note="catalyzes the formation of malonyl-CoA from
                     malonate and CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HIR6448288.1"
                     /translation="VAVVVPENEEVSERSVAAGLDGQLARFKQPKRVINIGELPRNAM
                     GKVQKNELRERFADLFAQS"
BASE COUNT          753 a         1413 c         1285 g          784 t
ORIGIN      
        1 cctgggccat ctgacgctgc tcatcggtac cacgagccat gaccgtcatg atttcgtaac
       61 cctggcgctg gtgaaaactc tcttccttgc agacccggac cattgccctg gagtaaggcc
      121 cgtaagaaca acggcacagg ggaatctggt tcatgatggc cgcgccatcg accagccagc
      181 cgatggcgcc gatgtccgcc caggtcggcg tcgggtagtt gaatatgctg gagtacctgg
      241 cctgcccgtc cagcagctgc tcgatcatct gctcccgcga gatgccgagc gtctcggcgg
      301 cgccgtacag ataggccccg tgaccgccct cgtcctgcac cttggcgatc agcaccgact
      361 tgcgcctgag cgtcggagcc cgggatatcc agttgccctc cggcaacatg cccaccacct
      421 cggaatgcgc gtgctgggaa atctgccgta ccagcgtcat ccggtaggca tggggcatcc
      481 agtccttcgg ctcgattttc tcgtccgcgt cgatcctggc ctgaaaacgg gcttcgagct
      541 cggccagctc gtcggaagga atgtgcaggc tttcgtcacc gagcgagtcc agaccctggg
      601 tatacatccg cgcatccaaa ttgtttgcac gcgccgattg taacccttcg cgcggggccc
      661 gactaacggg ccggcaagcg gcgcctaggg cgtgtgagcg accagaaact ccagcacccg
      721 ggcacgcgcc gcctcctgat tctccggttc cttccagcgc tcgatgagct cggcgttggg
      781 agccagcgcg acgatttccc gcgacgtgct ctcaggatgg tagagatcgc gaccgcagag
      841 cacgaggagg ggcgtcgaac acccggccac gaactcccgc gatacgttga agagaaagtc
      901 ccctgcgtac atggaccgct tgaacgcctc ccaagcctcg gggtccacgt cgtccctgtc
      961 cggcttcagc tcttccgccc agctgtcgaa catgtcgaag aacgcctgcc tgttgtcgtc
     1021 caggccgatg ggctgaaaca gcacggccgc agcgacccgg tccggcaccg cacggaccag
     1081 gcccatacag tacggaccgc caatgcacat gccggccacg tggaactggt ccactccgag
     1141 gtgatccagc agcgcgatct gatcctcggt gtagacctgc cagccgtccg tggagcggat
     1201 gggcgcccgg gaatggcccg cgttccgctg gtccatgacg atcactttga agtggggctc
     1261 gagatgctcg atgggattcc acggagcatt tgcccacgcg ccgatggtcg agcgcatacc
     1321 accgggggct atcagcagga tggggaatcc gctgccgcgc tcctcgtagt gaatgctcac
     1381 gccgccccgc tcgaaggtgg ccatgggatc agccctcttc tagcacggcc tggaaggtct
     1441 cgagattcgg atggccgaga taggtcccct tcggcgcgct tgcctgggca tgggcgcgcc
     1501 ggaagttgtc ggactcggtc caggcttcga aggcttcccg ggattcccag acactgtgcg
     1561 atgcgtacag cgtgtgatcc tcgttctcgg gtccacgaag cagcgcgaag tgcttgaagc
     1621 ccggcacgtc cgacagaaag ctttcccgct cccgccagac tttttcgaat ccctcctcga
     1681 accctttggc gatctgaaat ctattcatgg ctatgaacat aggtgccctc ctgctcgggt
     1741 tgtctgtgac gatcagcgtc gatccgctga aatagttcgt gcagctgtcg ggcgctctgc
     1801 acgacgcgcc cggctgccag cctgccctgg tcgtcaatga ttgcgatgct cggcagctgc
     1861 tgcacctgca ggtgcagggc cagctcctgg gaaatcaggt acctttccag gtcgatgccg
     1921 ttggcttggg cgtaagccgg gtaggcgggc atgtcggaac catcactcac ccagtaaccc
     1981 tccagccacg gctcgcgatc ccgcagcatg tccaggtagg cgggcagcac ggagcgacag
     2041 atcggacact cggcggcgat gaagagcaac gccgaccatc gcccagcgtc ggccgcatct
     2101 ttgagcccga catgggcggc cgtgagcgtc ggcaactcga ggtcgggcac acggccaccc
     2161 tcttccacac cctggctctt cgacacgaga cccagcggcg ccgtgcgttc gtggagaatg
     2221 ccgatctgac gggcaagcga gagaaccacc agcgcaaggg cacccacaac ggccaacagc
     2281 aggaggtttg agaccaacag ccaagtcatg accagggagt gtaatccacg ccgcctccgg
     2341 tacgataccg ccatggaaat cgacctctgg cgtccgctgg cggggctaag cctgttcctg
     2401 ttctccatga gcatcatcga gcgcgcttta gaggacgtct ccggccgagc cttccgcaga
     2461 tttctccgcc gcaacacaga ccatcccatc aggggcatcg gcagcggcac gctggcaacg
     2521 gccatccttc agtcgagctc cctggtcggc gtgatgatgc tcgccctggt gggcgccggc
     2581 gtgatccaga tgcgcaacgc cctgtctgtg atattcggtg ccaatctcgg caccaccatg
     2641 accgggtgga tcgtggcaac catcggcttc aagctcgacc tcgacgcgct ggcgctgccc
     2701 ctcatcgccg ccggcggtct tgctgccatg ggcattcgcg atcatcgcgc atcccagatc
     2761 gcccgcgtcg tcttcggggt gggtctgctg ctcatgggcc tcgacctgat gaagggcgcc
     2821 gtgggcaaca tatcggatgc cattgacgtt gccgttctcg gggagctcgg cgcggtggag
     2881 tatctcctct tcgggctcgt cttcaccgcc atcgtccagt ccagctcggc aaccatgatg
     2941 gtgaccttga gcgcgctgca cggcggcatc atcgacctcc ctgcggcagc tgcagtggtc
     3001 atcggggccg acctcggcac cacgggtacc gtgctgctgg cggccttgag gggcagcgcc
     3061 gccaaaaaac gggttgccat gggccacttc ctgttcaatc tgatcaccga tctcacggcc
     3121 ttcgcgctgc gaacgccgtt gctggccgtg atcctcatga tcgggttgtc agatctgctc
     3181 gctctggtcg ccttccacag cgcgttcaat ctgatcggga tcctgatgtt cctgccgttg
     3241 atcggtcggt tcgccggttg gctcgaagga cgtttcaccg acaccgagac ctcggtgagc
     3301 cgccacctcg ccgatgtcgc cgtcgatctg cccgaggccg cagtagaggc catccgcctg
     3361 gagaccaatc acctcatgca acgggtgatc tgccagaacc taaaggtgcc ggatcccgag
     3421 atatcgttgc ccgcgggttc gctaccggtg ggagtgcacc aagccggcgc catcgccgcc
     3481 gcgctggaac ccgagttcga agcggcatat gcggcaagca aacgcctcga gggcgagatc
     3541 atcacgtttg ccgcggacgc ccaggcggca tcactggaga agagcgaaag cgacctcatc
     3601 acgcgatgcc agtttgccgc ccgcgaagcc gtccacgcat cgaagagtct caaggacgcg
     3661 agaggcgacc tcagagcgtt cgccctctcc catcggccca ttctgaacgg ctacggcaac
     3721 cgctttcgtg aaatcatgag cgagttctac gcacgactct tcctgcttcg tcacgggccg
     3781 acggcagaac gggcggacgg agggccggag ctcgaagatc tgatcgtcct cagccagctc
     3841 gctcatcaac gccacgagga gctgcacacg gcaatctacg aggacgtccg ggcggaccgg
     3901 ctggacgagg acgagatctc ctcgctgctc aatgccaacc gtgagctgtt caactcgaac
     3961 cgggcgctgc tttcggccat cgccaccatt accctgccgc cggagcaggc ggccacgttc
     4021 gacctgctgc cgaccagcgt ggtctagctt tgcgcgaaga ggtcggcgaa acgctctcgg
     4081 agctcgttct tctgcacctt gcccatggcg ttgcggggaa gttcgccgat gttgatcacg
     4141 cgtttgggct gcttgaatcg agcgagctgg ccatccagcc ccgccgctac cgaccgctcg
     4201 ctcacctctt cgttctccgg aacgaccacg gcgac
//
LOCUS       DBNHLM010000145         7506 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_81284, whole genome shotgun sequence.
ACCESSION   DBNHLM010000145 DBNHLM010000000
VERSION     DBNHLM010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7506)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7506)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7506
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_81284"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1837
                     /locus_tag="AC16QF_06750"
     CDS             242..1837
                     /locus_tag="AC16QF_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003379714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HIR6448289.1"
                     /translation="MVATARTAALAGGSDRRTFAIISHPDAGKTTITEKLLLFGNAIQ
                     LAGTVKARKSDRHATSDWMAMEQQRGISVTTSVMQFPYRDRVVNLLDTPGHEDFSEDT
                     YRTLTAVDSAIMVIDGSKGVEPRTIKLMEVCRLRDTPILTFINKLDREIRDPVEVMDE
                     IEDILQIQCAPMTWPIGMGRQFKGIYDLYADEIVCFVQGHGHHIFDYPRIKGLESNAA
                     RELLGSDHDDVVEEIELVRGASHAFDVDDYLGATLSPVYFGTALGNFGVKEMLDGFVT
                     YAPPPQARHADARDVKPGEEQFSGFVFKIQANMDPKHRDRIAFLRVCSGRYAQGMRMR
                     HVRLGKDVKVADAVTFLAGDRAQTVEAFPGDIIGLHNHGTIQIGDSFTQGEVIKFTGI
                     PNFAPELFRRVRVKDPLRSKQLERGLDELSEEGASQVFKPLIRNELIVGAVGMLQFDV
                     VAFRLREEYGADCVYDSTNIFAARWVVSDDKKKLEDFTRKHRDQLAVDGGGHLAYLAP
                     TRANLQLVEERWPEIRFLKTREH"
     gene            1838..2629
                     /locus_tag="AC16QF_06755"
     CDS             1838..2629
                     /locus_tag="AC16QF_06755"
                     /inference="COORDINATES: protein motif:HMM:NF024762.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HIR6448290.1"
                     /translation="MAGLAEQELIQAVRAFASDPEADEHGRFELVNRALEPSLDADTL
                     RGRIAELAAACPETQTPWDYLRELGFVGNAANYDALDNSRLDRLLDTRRGIPIALGVL
                     LIEVARASGRMAVGVNFPGHFLVRVGESLVDPFRMTQTSEDECVAGLGQARGNPGAGG
                     DLFPSATAAAVLLRMLNNIKYRYAQAGEWHRSLDMVDLQLAAAGESAHLLLERGELWL
                     RLGARGPARADLERALALAGDGDPRLAAAARRLLDGLGSGPELLH"
     gene            complement(2647..4500)
                     /locus_tag="AC16QF_06760"
     CDS             complement(2647..4500)
                     /locus_tag="AC16QF_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HIR6448291.1"
                     /translation="MIRLPVIVGFGGINPAGRASFDHAYRRLVIDALDEASRARTYRS
                     LASLMNIHDDPDEVSTRAYIDAHTLIRRIELFDPNEVPVQRTARLAHPEDEPIRFVLA
                     TRQLPERIPAGWQVSSLTEQQVLVTVDNELDVLLPDTRISRVSSAGQAPTGFDPGALY
                     QSRNHPRGLQLTVFGASDALRSTGIPIEELKQRVAPDQFAVYSGSAMGQLDADGYGGM
                     LQNPLMGKRPTSKNVALGLSEMPGDFVNAYVLGSVGDTAGIIGACATFLYNMQHGIEE
                     IRSGRKRVVMVGNSEAPVVPDVIEAYRVMGALAEDEALMALDSRESEPDHRRACRPFS
                     DNCGFTVAEASIFTVLMDDELALQLGARILGSVGGVYVNADGYKKSIPGPGIGNYLTV
                     AKAMSLSRKLLGDESLQHRTYMLAHGTGTPQNRVTESHILNELAKVFGIERWPVAAIK
                     AYVGHSMAPAGGDQLAAALGLWQHGWLPGITTIDHIADDVHDSNLNLPLEHLELGAEN
                     VDAAFVNSKGFGGNNATGLFLSPAKTREMLRKRWGKKALAEHGRSNESVEQAALDYDE
                     AMVRGEAKPIYQFGEGVVHGEDLTISRDSIHIPGFGMPVDLDLENPFPDMT"
     gene            complement(4648..5790)
                     /locus_tag="AC16QF_06765"
     CDS             complement(4648..5790)
                     /locus_tag="AC16QF_06765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6448292.1"
                     /translation="MTEQAYIVNAARTPVGSFQGALAHLTAPELGAHAIRALAPANVD
                     EVIMGNVIAAGLKQGPARQAMRMAGLPDACGATTINKLCGSGMKAAMLATDLIRAGSA
                     ARVVAGGMESMTNAPYLIPKARAGMRMGHGELKDAMFLDGLEDASTGAAMGTFAQATA
                     DEHQLSREAMDEFAIESVTRARWAITNQELASEISPIMINGDEVRDDEQPGRAKVERI
                     PSLRPAFKPDGTITAANSSSISDGASALLIAGESALEGAEPMARIIAHATHSRHPSEF
                     TVAPADAIRQVLDRAGWKADDVDVFEINEAFAMVTMLAIQTLNLDPSRVNVYGGACAQ
                     GHPVGSSGSRLIVTLAHALERLNKKRGVAALCIGGGEATAIAIERP"
     gene            complement(5795..6307)
                     /gene="moaB"
                     /locus_tag="AC16QF_06770"
     CDS             complement(5795..6307)
                     /gene="moaB"
                     /locus_tag="AC16QF_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017131799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HIR6448293.1"
                     /translation="MPDLTSLNCAVLTVSDTRTLEDDRSGDLLIELLTADGHRLAHRD
                     IVRDDVYAIRAIVSRWIADESVSVILTTGGTGFTGRDSTPEALIPLFDKTIDGFGELF
                     RQVSHAEIGASTIQSRTVGGIANATLIFALPGSSNAVRTGWEKILHHQLDVNFKPCNF
                     VELIPRFLER"
     gene            6444..>7506
                     /locus_tag="AC16QF_06775"
     CDS             6444..>7506
                     /locus_tag="AC16QF_06775"
                     /inference="COORDINATES: protein motif:HMM:NF015228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR6448294.1"
                     /translation="MDKQLVEGDRLYLIAESLAQDFSLFPTAESQERLPGLLSPRDIL
                     RQKQRLANLDVDAYIEAVVARAEDPGRTAAPVVIRQLGRVVEEVSDGPFYAATVEMDF
                     DGEPRLVGFIAQDRSSRNGVWMPAHHLAAANRIQNYAKRSQPIVTMMDTPGADAEEQA
                     NRENQAHSISRLIAEMSNVDVPNVGIIYGLGYSGGAIPLAASNMILSVRDGVFSTIQP
                     KSLANIARRLNLSWQECAKYVGLSPYELYAQGNIDGVIDYVPDDDVANLRLAIVSGVQ
                     QVEEKVKEFVAENPYILDHYRQSLQRYLNPSEQLKVMQASASMKLTKNPTEYLNVFGV
                     AYRYLRYLKVRQRIKATSTH"
BASE COUNT         1252 a         2359 c         2514 g         1381 t
ORIGIN      
        1 tcggccatgt tcctcgaggt ccggccctct aatctggtgg ccgccgaact gtacgagtcg
       61 ctcgggttcc gggaaatcgg aattcgcaag aattactatc cggcccaggt gggtcatgag
      121 gacgcccggg tactcgcgtt ggatcttgat gctcatttcc gcagcgaccc gctataatcc
      181 gcgccgtttt ccggcggggc cgtccggctt cggaaaagtc ggtcgctgag gagtctttat
      241 catggtggca acggcccgga ccgccgcgct cgcggggggg agcgatcgac gcacctttgc
      301 gatcatctct catccggatg ccggcaagac cacgatcacc gagaagctgc tcctgttcgg
      361 caacgccatc cagctcgccg gcacggtgaa agcgcgcaag tcggaccgcc atgcgacctc
      421 cgactggatg gccatggaac agcagcgggg gatttccgtc accacctcgg tgatgcagtt
      481 tccctatcgg gaccgggtgg tgaacctgct cgataccccg gggcacgagg acttctccga
      541 agatacctat cgaacgctca ccgcggtcga ttccgccatc atggtcatcg acggctccaa
      601 gggcgtcgag ccgcgcacga tcaagctcat ggaagtctgc cggctgcgcg atacgccgat
      661 tctcacgttc atcaacaaac tcgaccggga gatccgggac ccggtggagg tgatggacga
      721 gatagaggac atcctgcaga ttcagtgtgc gcccatgacc tggccgatcg gcatgggccg
      781 ccagttcaag ggcatctacg atctgtacgc cgatgagatc gtctgcttcg ttcaggggca
      841 cggccatcac attttcgact acccgcggat caagggcctg gagagcaacg ccgcgcgcga
      901 gctgctgggt tcggaccacg atgatgtggt cgaagagatc gagctggtac gcggggccag
      961 ccacgcgttc gacgtcgacg actatctcgg cgcgacgctg tcgcccgtct acttcgggac
     1021 cgcgctcggc aacttcggag tcaaagagat gctcgacggg ttcgtcacgt acgccccgcc
     1081 cccccaggcc cgacacgccg acgcccgcga cgtgaagccg ggggaggaac agttcagcgg
     1141 gttcgtgttc aagatccagg cgaacatgga cccgaagcac cgcgatcgca tcgccttcct
     1201 ccgggtgtgc tcgggccgtt atgcccaggg catgcggatg cgccacgtgc ggctcggaaa
     1261 ggacgtcaag gttgccgatg cggtgacctt tctcgccggt gaccgggccc agacggtgga
     1321 ggcgtttcct ggcgacatca tcgggcttca caatcacggc accattcaga tcggcgattc
     1381 cttcacccag ggtgaggtga tcaagttcac cgggatcccg aactttgcgc ccgagctgtt
     1441 ccgacgggtg cgggtaaagg atccgctcag gagcaaacag ctggagcggg ggctggacga
     1501 gctgtcggag gagggagcca gtcaggtttt caagcccttg atccgcaacg agctcatcgt
     1561 tggtgcggtg ggcatgctgc agttcgacgt ggtggcgttc cgtctgcgcg aagagtacgg
     1621 ggctgactgt gtctacgatt cgaccaacat ctttgctgcg cgctgggtgg tctcggacga
     1681 caagaaaaag ctcgaagact tcacgcgcaa gcaccgcgat cagctggccg tggatggcgg
     1741 aggacacctt gcttaccttg ctccgacccg ggcgaacctg cagctggtgg aggagcgctg
     1801 gcccgagatc cgctttctca agacccggga gcattgattg gccggcctcg cagagcagga
     1861 actgattcag gcggtcagag cgtttgcctc ggaccctgaa gccgacgagc acggccgctt
     1921 cgagctcgtc aatcgcgctc tggagccgtc tctggatgcg gacaccctgc gtgggcgcat
     1981 tgccgagctt gcagcggcct gtccggaaac gcagacaccc tgggattacc tccgggagct
     2041 tggattcgtc ggcaatgccg cgaattacga cgcgctcgac aacagccggc tggaccgtct
     2101 gctcgacacc cggcggggca tacccatcgc cctgggcgtc ctgctgatcg aggtggcgcg
     2161 cgccagcggc cgcatggccg tcggcgtcaa ctttcccggg cactttctgg ttcgggtggg
     2221 agaaagtctg gtagatccgt tccggatgac gcagacctcc gaggacgaat gcgttgccgg
     2281 tctcggccag gcgcgaggga acccgggcgc ggggggcgat ctctttcctt cggccaccgc
     2341 ggcggccgtc ctgctgcgca tgctcaacaa catcaagtac cgctacgccc aagcggggga
     2401 gtggcatcgc agcctcgaca tggtggatct gcagctagcg gccgcggggg agagcgcgca
     2461 tctgcttctc gaacgcggtg agctgtggtt gcggctaggc gccaggggac cggcacgtgc
     2521 cgatctggag cgcgcgttgg ctcttgctgg ggacggcgat ccccgcttgg ccgccgcggc
     2581 tcgacggttg ctcgacgggc tggggtccgg cccggagctg ctgcactgat tgcggggctc
     2641 agcgcctcag gtcatgtccg ggaaaggatt ttccagatcg aggtccacgg gcattccgaa
     2701 gccgggaatg tggatgctgt cgcgactgat cgtcaggtcc tcgccgtgaa cgacgccttc
     2761 gccgaactga tagatcggct tggcttcgcc gcgaaccatg gcctcgtcgt agtccagggc
     2821 ggcctgctcg acgctttcgt tgctgcggcc gtgctcggcc aacgccttct tgccccagcg
     2881 cttgcgaagc atctcccggg tcttcgccgg tgacaggaac agcccggtgg cattgttgcc
     2941 accgaaacct ttggagttga cgaaggcggc atccacgttc tcggcgccga gctccaggtg
     3001 ctccagcggc aggttcagat tgctgtcgtg aacgtcgtcg gcgatgtgat caatggtggt
     3061 gatgcctggc agccagccgt gctgccacag gcccagggcg gcggccagct gatcgcctcc
     3121 cgcgggcgcc atggagtggc ccacgtaggc cttgatggca gctacgggcc agcgctcgat
     3181 accgaagacc ttggccagct cgttgagaat gtgcgactcg gtcacccggt tctgtggcgt
     3241 cccggtcccg tgggccagca tgtaggtgcg gtgctgcagg gactcgtcgc cgagcagctt
     3301 gcgggagagg ctcatggcct tggccaccgt gagatagttg ccgatgccgg ggcccgggat
     3361 tgatttcttg tacccgtcgg cgttgacgta gacgcccccc accgagccga ggatgcgggc
     3421 gccgagctgc aatgccagct cgtcgtccat cagaacggtg aagatgctcg cctccgccac
     3481 ggtgaatccg cagttgtcgg agaacggtcg acaggcgcgc cggtgatcgg gctcggactc
     3541 ccggctgtcg agcgccatca acgcttcgtc ctcggcgagc gcgcccatga cccggtacgc
     3601 ctcgatgacg tctggcacca cgggcgcctc ggagttgccc accatcacca cgcgcttgcg
     3661 cccggagcga atttcctcga tcccgtgctg catgttgtac aggaaggtcg cgcaggcgcc
     3721 gatgatgccc gcggtatcgc cgacggaccc gagcacgtag gcattgacga agtcgccggg
     3781 catttccgac agcccgagcg cgacgttctt cgacgtgggg cgtttgccca tgagcgggtt
     3841 ctgcaacatg ccgccgtagc cgtcggcgtc cagctgcccc atggcgctgc ccgagtagac
     3901 ggcaaactga tcaggcgcga cccgttgctt tagctcctcg atgggtatgc ctgtggagcg
     3961 cagggcgtcc gacgcgccga acaccgtgag ctggagcccg cgcggatggt ttcgcgactg
     4021 gtacagagcg ccgggatcga agccggtagg tgcctggcct gcgctgctga ccctggagat
     4081 tcgagtgtcg ggaagcagca cgtcgagctc gttgtctacc gtcacgagca cctgctgctc
     4141 ggtgagggaa gagacctgcc agcccgccgg gatgcgctcg ggcagctgcc gcgttgccag
     4201 aacgaaacgg atgggctcgt cctccggatg ggccagcctg gcagtgcgct ggacgggaac
     4261 ttcgttggga tcgaacagct ctatcctgcg gatgagcgtg tgggcgtcga tgtacgcccg
     4321 ggtggagact tcgtccgggt cgtcgtgaat gttcatcagg ctcgccaggc tgcggtaggt
     4381 gcgggcccgg gaggcctcgt cgagagcgtc gatcaccagg cgtcgatagg cgtggtcgaa
     4441 ggacgccctc cctgccgggt tgatgccgcc gaaccccacg atgaccggaa gccggatcac
     4501 tcatactctc cgttgctggc aacgcgcttg gggtcgccgg atgcaggatg cttctcgcgc
     4561 agcgcgcccg cttgcgggag gacgcgcgct cgccagatca ccggagaatt gtagggagac
     4621 gcctgcgcca tttccatcag tgtgacgtca tggcctctcg atggcgatgg cggtcgcttc
     4681 gccgccgccg atgcagagcg cggccacgcc ccgcttcttg ttcaggcgct ccagcgcgtg
     4741 cgccagcgtg acgatcaggc ggctgccgct ggatcctacg gggtgtccct gggcacacgc
     4801 gccgccgtag acgttcacgc gcgacgggtc caggttcagc gtctggatcg ccagcatcgt
     4861 gaccatggcg aaagcctcgt tgatctcgaa gacgtccacg tcgtcggcct tccatcctgc
     4921 gcggtcgagc acctgccgga tggcgtcggc gggggcgacg gtgaactcgc tcggatgccg
     4981 ggaatgcgtg gcgtgggcga tgatccgggc cattggctcg gccccttcga gggcggactc
     5041 gccggctatc agcaacgcgg acgcgccgtc tgaaatggag ctggagtttg ccgcggtgat
     5101 ggtgccgtcg ggcttgaagg cgggccgaag gctcggaatc cgctcgacct tggcccggcc
     5161 gggctgctcg tcgtcgcgca cctcgtcgcc attgatcatg atcgggctga tctcgctggc
     5221 gagttcctgg ttcgtgatgg cccatcgtgc ccgggtgacc gactcgatgg cgaactcgtc
     5281 catggcctcg cggctgagct ggtgttcgtc cgcggtcgcc tgggcgaagg tgcccatggc
     5341 cgctcccgtg ctcgcgtcct ccagaccgtc gagaaacatg gcgtctttga gctccccatg
     5401 gcccatgcgc atgcccgccc gcgccttggg aatgaggtag ggcgcgttgg tcatgctttc
     5461 catgcccccg gcgaccacgc gcgcggcgct gcccgcgcgg atcagatcgg tggcgagcat
     5521 ggcggccttc atgccggagc cgcacagctt gttgatggtc gtcgcgccgc aggcgtcggg
     5581 cagcccggcc atgcgcatcg cctgacgcgc gggaccctgt ttcaggccgg cggcgatgac
     5641 gttgcccatg atcacctcgt cgacgttcgc gggcgcaagc gcacggatcg cgtgggcgcc
     5701 gagttccggc gcggtgaggt gggcgagcgc cccctgaaag ctgcctacgg gcgttctcgc
     5761 ggcgttgacg atgtaggcct gttcggtcat gagcttatct ctccaggaat ctgggaatga
     5821 gttctacgaa gttgcagggt ttgaaattca cgtcgagctg atgatgcagg atcttctccc
     5881 agcccgttcg gacggcgttg ctcgacccgg gcagcgcgaa gatcagcgtg gcgttggcga
     5941 tgccgcccac ggtgcgcgac tggatggtcg atgctccgat ctcagcatgg gaaacctggc
     6001 ggaacagctc gccgaagccg tcgatggtct tgtcgaacag tgggatcagc gcctccggcg
     6061 tgctgtcgcg tcccgtgaag ccggtgcctc cggtggtgag gatcaccgat acgctttcat
     6121 cggcgatcca gcgggagacg atggcgcgaa tggcgtagac gtcgtcgcgg acgatgtcgc
     6181 ggtgggcaag gcggtgaccg tcggcggtga gcaactcgat caggagatcg cccgatcggt
     6241 cgtcttccag tgtgcgggta tccgacaccg tgagcacggc acaattcagg gaagtcaggt
     6301 cgggcacggg ccgcgtcctc ctttgctgct ccggcgcaga tgcgaatttt ctagaaaaat
     6361 cggccactta gcgagcgtgg attgcttctc atttggcaag tggctagagt accggcgaat
     6421 tctacaccct taacgatcgg cccgtggata aacagctcgt cgaaggtgat cggctctacc
     6481 tgattgcgga aagcctggcg caggactttt ctctgtttcc caccgcggag agccaggaac
     6541 ggctcccggg gctgttgtcc ccgcgtgaca tcctgcggca gaaacagcgg ctcgccaatc
     6601 tcgacgtgga cgcctacatc gaggccgtgg tcgcgcgagc ggaggatccg ggccgcaccg
     6661 cggcgcccgt ggtcattcgg cagctcggac gggtcgtcga ggaagtttcc gacggtccct
     6721 tttacgctgc cacggtggag atggatttcg acggcgagcc gcgcttggtc ggcttcattg
     6781 cccaggatcg ctcctcccgc aacggcgttt ggatgccggc gcatcatctg gccgctgcga
     6841 accgcatcca gaactacgcc aagcgctccc agcccatcgt cacgatgatg gacacgcccg
     6901 gcgccgatgc tgaggagcag gcaaaccggg agaatcaggc tcacagcatt tcccggctca
     6961 tcgccgagat gagcaacgtg gacgttccca acgtcggcat catctatggt ctcggctact
     7021 cgggcggcgc gataccgctg gccgccagca acatgatcct gtcggtccgc gacggggtct
     7081 tcagcaccat ccagccgaaa agcctggcca acatcgcgcg tcggctgaac ctgtcctggc
     7141 aggagtgcgc caagtacgtt ggcctgtcgc cctacgagct ctatgcccag ggcaacatcg
     7201 acggggtcat cgactatgtg cccgacgacg acgtggccaa cctccgcctg gccatcgtgt
     7261 ccggggtgca gcaggtcgaa gagaaggtca aggagttcgt cgcggagaac ccctacatcc
     7321 tcgaccacta ccggcagagc ctgcagcgct acctgaaccc ttcggagcag ctgaaggtca
     7381 tgcaggccag cgcgtcgatg aagctgacga agaaccccac ggagtatctc aacgtcttcg
     7441 gcgtggcgta ccgctatctg cgttacctca aggttcgaca gcgtatcaag gccaccagca
     7501 cccacc
//
LOCUS       DBNHLM010000146         4134 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_440010, whole genome shotgun sequence.
ACCESSION   DBNHLM010000146 DBNHLM010000000
VERSION     DBNHLM010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_440010"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="AC16QF_06780"
     CDS             <1..784
                     /locus_tag="AC16QF_06780"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR6448295.1"
                     /translation="LLHYSELCRHGVEERTGMPTALAMRYGTPSVRDAIAALLGHGVR
                     QITMLPLYPQHADSTITTSIEAVRRSLPTNVSLTVVPPFYSQPDYLDAQAAAIRENLP
                     ASWDHLLFSYHGLPERHITKADPTGNHCLALENCCQIPSEAHATCYRHQVYRTSELLA
                     QRLGLSEDQYSVSFQSRLGRLPWLTPYTDQVLEALPGKGVRRLAVTCPAFVADNLETL
                     EEIGIAGRESFLAAGGESFSLVPCLNDRADWIDAIVRIAGAA"
     gene            complement(838..1692)
                     /locus_tag="AC16QF_06785"
     CDS             complement(838..1692)
                     /locus_tag="AC16QF_06785"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6448296.1"
                     /translation="MIENLETLVTLARTGTMMETSTVLKISQSAVSKRIAALERYYDR
                     DLIERHGRRVVLTHHGTRLVEKVTPVISELRSVFLEDNALRKGKIIIGVSEAILSSWG
                     PRLFARVRSELPEVELEFHAQRSPVVLDRIRSGEYMVGICTGSPDADTDLQSEVIRLE
                     PMVIIPSALAPLDYEVGDELDVITIESRSGAWRSIEDDMRRQNLRRVVPLESFFGVAQ
                     MALAGFGHGLVPEGVAQTLGVTAGERIPLDSAGLNRPVRFVARKSMFSQQLVQSFYQA
                     VSRFAAEV"
     gene            1798..2532
                     /locus_tag="AC16QF_06790"
     CDS             1798..2532
                     /locus_tag="AC16QF_06790"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR6448297.1"
                     /translation="MWSTLLLASVFATSVLSGVLGMAGGMILMAILVSLMSVPAAMII
                     HGVVQATSNGSRAWFLRAHIRWRVLPPYLLGAAVTLAAFAALTLVPEAGLVLVLVGIF
                     PWLARVVPRLQGLDVERPATAVACGATVTAAQLFAGASGPLLDVFYLNSSMDRYQVIA
                     SKAFTQTIGHGLKLAYYGTIIGVAETLPWWMFGAAMATAVAGTRVGTRLLGRLRDDVF
                     RRVSGWVILAIAAVCVVKGGMELAGW"
     gene            complement(2551..>4134)
                     /locus_tag="AC16QF_06795"
     CDS             complement(2551..>4134)
                     /locus_tag="AC16QF_06795"
                     /inference="COORDINATES: protein motif:HMM:NF014805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6448298.1"
                     /translation="VCIGGNTPDESYLNAMSVLSVAEHEGTDALHPGIGFLSENSQFA
                     ELVRSHGINFIGPPVSSMETMGNKSNAINTALRLNVPVVPGSHGILTNVERAAEVAEE
                     IGYPVLIKAVHGGGGKGIQVVESRDSFHELFHRVTVEARAAFGNGDVYLEKYVTSLRH
                     IEAQLLRDVHGNTRVLGIRDCSVQRDKQKVIEESDSTVLPDRLREEVLQHTAALADEV
                     GYVGAGTVEFIYDLKSDAVYFMEMNTRLQVEHPVTEWVSGVDIVSQQFRIAAGESIAD
                     LDPSPRGYSIEARVTAERVQAAPGGELVFRPQPGQVAECSFPEDEGVEIIAAAAPGKF
                     ISPYYDSMIAQVVVYADDRAQAIEKLAAYLRRVRVTGICTNIPLLKHILDDEVFQQGV
                     YDTGYLPGLLARLDADELIAEIEGGAGEVSGGIDMNAIRIDESDELKVLSPATAIFYG
                     TPTPTEPPYVSVGDRVKLSDTLCQLEAMKIFTPLSLADFNSEFELYEAGREYEITRIN
                     MGTGQQVNAGDLLFVVRPV"
BASE COUNT          706 a         1381 c         1279 g          768 t
ORIGIN      
        1 ccttctgcat tactcggagc tatgccgtca cggggtcgag gaacgtacgg ggatgcctac
       61 tgctctcgcc atgcgctacg ggacccccag cgtgcgggac gccatcgccg ccctgctcgg
      121 acatggcgtg cggcagatca ccatgcttcc cctgtacccg cagcacgccg actccaccat
      181 caccaccagc atcgaggccg tacgacgaag tctgccaacc aacgttagcc tgaccgtggt
      241 gccgcctttc tactcgcagc cggactatct ggatgcccag gccgccgcga tccgagagaa
      301 cctgccggcg tcctgggatc acctgctctt cagttaccac ggtctgcccg agcggcacat
      361 caccaaggca gatcccaccg gcaaccattg ccttgccctg gagaactgct gccagatccc
      421 ctccgaggcg cacgccacct gctaccggca tcaggtctac cgcacctccg agctccttgc
      481 ccaacgcctg ggcctctccg aggatcagta cagcgtgagc tttcagtcgc ggctgggacg
      541 gctgccgtgg ctcaccccct ataccgacca ggtgctggaa gccctgccag gaaaaggggt
      601 caggcgtctc gccgtcacct gcccggcctt cgtcgcggat aatctggaaa ctctggagga
      661 aataggcatc gccgggcgcg agagcttcct ggctgccggc ggagagtcgt tctcgctggt
      721 tccctgcctg aacgaccgcg cggactggat cgacgccatc gtccgaattg ccggggcggc
      781 ctagagcccg cctccggcca catcggtcat caccggaaaa acgggtcttc gaagcggtca
      841 cacctcggcg gcgaaacggg aaaccgcctg gtagaaactc tgtaccagct gctgcgagaa
      901 catggatttc cgggcaacga aacgaacggg ccggttcagc cctgcgctgt ccagcggtat
      961 ccgttcgccg gccgtcaccc ccagcgtctg ggcaaccccc tccggcacca gcccgtgacc
     1021 aaaaccggcc agggccatct gcgccacgcc gaaaaatgac tccagcggca cgacgcgcct
     1081 gaggttctgc cggcgcatgt cgtcctcgat ggagcgccag gcacccgagc gcgactcgat
     1141 ggtgatcacg tccagctcgt cacccacctc gtaatcgaga ggcgccagcg cagacgggat
     1201 gatgaccatg ggctcgagcc tgatgacctc gctctgcagg tccgtatccg cgtccggcga
     1261 gcccgtgcag atgccgacca tgtactcacc ggagcggatc cggtcgagca ccaccggcga
     1321 acgttgggcg tgaaactcga gctccacctc cggcagctcg gagcgcaccc gggcaaacag
     1381 tcgagggccc caactggaga ggatggcctc ggaaacgccg atgatgatct tgcccttgcg
     1441 cagcgcgttg tcctccagga acacgctgcg cagctcggag atcaccgggg tgaccttctc
     1501 caccagccgc gttccgtggt gggtgagcac cacccgccgg ccatggcgct cgatcagatc
     1561 gcggtcgtag tagcgctcga gcgcggcgat gcgtttgctc acggcggatt gggaaatctt
     1621 cagcaccgtg ctggtctcca tcatggtgcc cgtgcgcgcc agggtgacca gagtctcgag
     1681 gttttcgatc atggcccgat tgaattgatt cgatttcggc gacgatcata ttccatccat
     1741 tctctttttt catggttatg gttggggcac cctgagcaca ttttgccggg cgtgatcatg
     1801 tggtcgaccc ttctgctcgc gagcgtcttc gcaaccagcg tgctctccgg cgtgctgggg
     1861 atggcgggcg gcatgatcct gatggccatc ctggtctccc tgatgtccgt gcccgccgcc
     1921 atgatcattc acggcgtggt tcaggccacc tcgaacgggt ccagggcctg gtttctgcgc
     1981 gcacacatcc ggtggcgggt gctgccccct tatctgctgg gcgcggccgt cacccttgcc
     2041 gcttttgcag ccctgacgct tgtccccgaa gcaggcctgg tgctcgttct cgtcgggatt
     2101 tttccctggc tggctcgcgt cgtcccgcga ctccagggcc tggacgtgga aaggccggcg
     2161 acggcggtgg catgcggtgc aacggttacc gcggcgcaac tattcgccgg ggcatcggga
     2221 ccgctgctgg acgtcttcta cctgaactcg tccatggacc gctaccaggt gatcgccagc
     2281 aaggcgttca ctcagaccat cggccacggg ctgaagctcg cttactacgg gaccatcatc
     2341 ggcgttgccg agaccctgcc ctggtggatg ttcggcgccg ccatggcgac ggccgtggcc
     2401 ggcacgcgcg tcggaacccg gctgctgggc cgcctgcgag atgatgtgtt ccgccgcgtg
     2461 agtggatggg tgatcctggc aatcgccgcc gtctgcgtcg tcaagggcgg gatggaactg
     2521 gccggctggt gagcgctgcc cgtgccctcc ctaaacgggc cgcaccacga acagcagatc
     2581 tcccgcgttc acctgttgtc cggtgcccat gttgattcgg gtgatctcgt actcccggcc
     2641 cgcttcatag agttcgaact cggagttgaa atcggccagc gaaaggggcg tgaatatctt
     2701 catggcttcg agctgacaga gggtgtccga cagctttacc cggtccccaa ccgacacgta
     2761 gggcggctcg gtgggcgtgg gcgttccgta gaaaatggcc gtcgcgggcg agagcacctt
     2821 gagctcgtcg ctttcgtcga tgcgtatggc attcatgtcg atgccgccgg aaacctcccc
     2881 ggcaccgccc tcgatttccg cgatgagctc gtcggcatcc aggcgggcga gaagccccgg
     2941 caggtagccc gtgtcataca cgccctgctg aaagacctcg tcatcgagga tgtgcttgag
     3001 caggggaatg ttggtgcaga tccccgtaac cctgacgcgg cgcagatagg ccgccagctt
     3061 ctcaatggcc tgggcacggt catccgcgta gacgaccacc tgagcaatca tgctgtcgta
     3121 gtagggggag atgaacttgc cgggcgctgc agcggcgatg atctcgaccc cttcgtcctc
     3181 cggaaagctg cactcggcca cctgcccggg ctgcgggcgg aagaccagct cgccgccggg
     3241 agcggcctgg acgcgctcgg ccgtcacgcg cgcctcgatg gagtacccgc ggggtgacgg
     3301 atcgagatcc gctatggatt ccccggcggc aatgcgaaac tgctgggaaa cgatgtcgac
     3361 gccggagacc cactccgtca ccggatgctc aacctgcaac cgagtgttca tctccatgaa
     3421 atacacggca tcggacttca ggtcgtagat gaactcgacg gtgcctgcac ccacataccc
     3481 cacttcgtcc gccagggccg ccgtgtgctg caggacctcc tcgcgaaggc gatcgggcaa
     3541 caccgtggag tcggactcct cgatcacctt ctgcttgtcg cgctgaacgc tgcagtcgcg
     3601 gatgccgagc acgcgggtat tgccgtgcac gtccctgagc agctgggcct cgatgtgcct
     3661 tagagaggtg acgtacttct ccaggtaaac atcgccgttt ccaaacgccg cgcgcgcttc
     3721 taccgtgacc cggtggaaga gctcgtggaa agaatcgcgc gactccacca cctggatgcc
     3781 cttaccgcct ccgccatgca cggccttgat gagcacgggg tatccgatct cctccgccac
     3841 ctcggccgcc ctctcgacgt tggtgaggat cccgtggctt cccgggacca ccggcacgtt
     3901 taggcgcagc gcggtgttga tggcattgga cttgttgccc atggtctcca tgctggacac
     3961 cggaggcccg atgaagttga tcccgtggga acgcaccagc tcggcgaact gggagttctc
     4021 ggaaagaaaa ccgatgcccg gatgcagcgc gtcggtgccc tcatgctcgg ccacgctcag
     4081 gacgctcatg gcgttcaggt agctctcgtc gggggtgttg ccaccaatgc agac
//
LOCUS       DBNHLM010000147        10930 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_81470, whole genome shotgun sequence.
ACCESSION   DBNHLM010000147 DBNHLM010000000
VERSION     DBNHLM010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_81470"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="AC16QF_06800"
     CDS             complement(<1..290)
                     /locus_tag="AC16QF_06800"
                     /inference="COORDINATES: protein motif:HMM:NF023144.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6448299.1"
                     /translation="MSTAEQIELARQVDDLRAEGAELAELMSKLTEEDWQRRTTFKDW
                     TIYDVIAHLHFSDHMGMTTIESGAAFQALMADMQSSGLPFVEYTRRWLGSVSG"
     gene            442..1701
                     /locus_tag="AC16QF_06805"
     CDS             442..1701
                     /locus_tag="AC16QF_06805"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6448300.1"
                     /translation="MTEPAAVRAEIPDPYSLPLDTLNVAQPALFQNDAHWGYFERLRN
                     EDPVHYCPQSDFGPFWSVTKFKDIFYVDTHHEIFSSEGGITLGPPVNQPPEEGFRPSM
                     FIAMDPPKHDVQRATVTPVVAPRNLAKLESVIRERAGRILDSLPRGETFDWVDTVSVE
                     LTTQMLATLFDFPFEERRKLTRWSDVATAIPGAGIVDSNAQRREELLECLDYFTRLWN
                     ERVNSEPGNDLISMLAHGEETRHMQPMEFLGNLILLIVGGNDTTRNSISGGVLALNQN
                     PDQYRKLRENPGLIRTMVPEIIRWQTPLAYMRRTAKVDTELRGKKIRAGDKVVMWYVS
                     GNRDGEVIDRPDEFIIDRPNVRHHLAFGFGIHRCMGNRLAEMQLRVVWEEILQRFHTV
                     EVVGEPERTYSSFVKGYTRLPVRLHEH"
     gene            complement(1740..3038)
                     /locus_tag="AC16QF_06810"
     CDS             complement(1740..3038)
                     /locus_tag="AC16QF_06810"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR6448301.1"
                     /translation="MSEYVFKLPDLGEGTVEAEIVEWHVRPGDEVNEGDLLVDVMTEK
                     ASVEVPAPVTGTVLRTSGMPGDLIAVGAELAAIETGASQETTKAPSTVGSAIALPESP
                     DVSMRPEPVEAPIEDAVTHPEPSSPSSADSDQVATLPDTPVESARRVITSPAIRRRAK
                     EAGVDLESLTGTGPRGRILQRDLEQAIAGRETAAPPASGTGAVNEVKVIGVRRVIAKR
                     LQAAVREIPHFTYVEEVDVTALESLRSHLNADRAEGRPALTYLPFIALALVRALKAHP
                     QCNAHFAAESGTLRLFDRVHLGVATQTSAGLKVPVVRNASERTLWDLASEMARVSEAA
                     RANTARPADLTGSTITVTSLGRLGGIVSTPVINAPEVAIIGVNKAVRRPVVVDDEVVP
                     RLMMNLSSSFDHRFVDGFDAASLIQAVKGYLEQPATVFIE"
     gene            complement(3035..4051)
                     /locus_tag="AC16QF_06815"
     CDS             complement(3035..4051)
                     /locus_tag="AC16QF_06815"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010955111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR6448302.1"
                     /translation="MAQMNMIQALNSALNIMLSRDPGVIVIGQDVGFFGGVFRVTDGL
                     QRQYGEHRVIDTPIAEGGIVGSAIGMGVNGLRPVAEIQFADYIYPGCDQIISELARLR
                     YRTGGDFFAPVTIRTPCGGGIRGGQTHSQSPEGIFTHVCGLKVVMPSNPYDAKGLLIS
                     AIEDDDPVIFFEPKRIYNGPFDGDANKPAVPWSTHPKGEVPEDYYTIPIGRAEVIRPG
                     SEVTILCYGTMVHVCAAAAEAAEVDAEIIDVRTMAPLDVETLKTSVEKTGRCVIAHEA
                     TRFSGYGAELAATVSEECFYHLEAPIQRVAGWDTPYPHAFEWEYFPGQKRVAAALERV
                     LEAP"
     gene            complement(4065..5303)
                     /locus_tag="AC16QF_06820"
     CDS             complement(4065..5303)
                     /locus_tag="AC16QF_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017642823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR6448303.1"
                     /translation="MSNKPRLHIPHPPARPGQEPDFSYVDISPAGAVPRPDVTTRLRD
                     IERSLAFEMVRVLDDDHAAVGSWNPHLDDHDLQIGLRHMLLTRLYDDRMLRMQRQGRI
                     SFYIKCTGEEAVAIAPAMALRPSDMLFPSYRTQGLQIARGRSLVDLMCHCLSNSRDMC
                     KGRQMPVMYHWARGNIFSVSGNLTTQFPQAVGWAMAAAIKGDDDVAAAWIGEGSSAEA
                     DFHHAMTFASVYLAPVILNIVNNQWAISTFQGFAGGERRPFSARGPGYGIPGIRVDGN
                     DFLAVHSVTSWAAERARRGVGPTLIEHVTYRASAHSTSDDPSRYRPRDEERSWPLGDP
                     IERLKQHMIREGYWSQAQHEQLVEELQAAVDEAWQEALSYGSMTEGPWLDRSEMFEDV
                     FKDVPAHLLAQREQLRALEE"
     gene            5534..6811
                     /locus_tag="AC16QF_06825"
     CDS             5534..6811
                     /locus_tag="AC16QF_06825"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6448304.1"
                     /translation="MAKDSESAGGSRVRMFHGWWIVAVAFCAQLLALGCSVAVYGLFI
                     PVLTDEFGASFMTANLGLSMLGVVMALAGALVGPLLDRRSIRGIMVGGAAISGLAFLL
                     MSYATELWQLAVLFGVAVAFGAAMFGPLAANTVIAKWFSRNRGRAVGIASMGPPTGGL
                     LLSPLVGVIMSSYGWRPTLLVFAGLHAILIPFLWFAIRNRPEDMNLLPDGARAMGSGA
                     TVGRGWTTADVFRSRNFWVLALAFGAVGAVAGAFNANVIPYARDIGIGLTEASLFISA
                     IGGTAIVGTVVFGTLADRMNITRLLLVSFLMQALAFFTLRSVAPGYGLLMLVVLVFGL
                     AAGALMPLMVAAVGRAFGPVSFGRVMGFIGPVTLPFAFVGPPLTGWIRQTTGSYLPAF
                     DLFFAVFVVCCGIVMMLKVPRATESAEENLRTG"
     gene            complement(6874..7443)
                     /locus_tag="AC16QF_06830"
     CDS             complement(6874..7443)
                     /locus_tag="AC16QF_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018926809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR6448305.1"
                     /translation="MKMQRHFLISDDLDDLEHLEEELEAQGVSTPQIHVFTLDDTEAE
                     KHVHLHDVQSIMKRDVVNSMIIGAILGAVLSASVLGATALLGWHQTAAGWIPFIFLAI
                     IVLGFCTWEGGFVGIQTLNRHFKQFKATLEQGKHVFYCDLAPDQVPVFQKIVRAHPSA
                     TMAGTGVSTPPWIISWQTRLTRFFGETMP"
     gene            complement(7471..7818)
                     /locus_tag="AC16QF_06835"
     CDS             complement(7471..7818)
                     /locus_tag="AC16QF_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007977898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HIR6448306.1"
                     /translation="MADTQAATHDAHGEGQQHPIKIYLWIWFLLFLFSAFSYGVDYMQ
                     FQGMLRWSLILIFMFLKAGFIVAIFMHMAWERLALITAILVPPLCLLILIGLMVIEGD
                     YTELTRVIFFQGD"
     gene            complement(7828..8520)
                     /locus_tag="AC16QF_06840"
     CDS             complement(7828..8520)
                     /locus_tag="AC16QF_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007993729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III family protein"
                     /protein_id="HIR6448307.1"
                     /translation="MANNVAAAGVPAFGEGEGWQGIVGDWSADKQAFPVPWGKAMMWI
                     FLLSDTFIFSCFLTSYMKVRISTTIPWPNPSQVFGLDVFGVSVPLLLIAIMTFVLISS
                     SGTMAMAVNFGYRRDKKKTVALMLATAGFGATFVGMQAFEWTKLISEGVRPWGNPWGA
                     AQFGSSFFMITGFHGLHVSAGVIYLVVVARRVANGFYDKKGNYDMVEISGLYWHFVDL
                     VWVFIFAFFYLW"
     gene            complement(8534..9157)
                     /locus_tag="AC16QF_06845"
     CDS             complement(8534..9157)
                     /locus_tag="AC16QF_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR6448308.1"
                     /translation="MKFLKQITEKSWLAPEEDVVADLRSGALVMGTPSAKIGLRVFLT
                     VVLVVFSLFCVAYYIRMELDDWRPLPEPDLLWINTVVILLSSVALQYTRYSVQRSRAG
                     AVNAGLLAAGLLTIGFLVGQYMAWEELRSAGYFAATNPANAFFYLLTAVHGLHLIGGL
                     VVWARTTLKVWNGAEPYQIRLSVELCTTYWHFLALVWLAILWILIST"
     gene            complement(9164..10906)
                     /locus_tag="AC16QF_06850"
     CDS             complement(9164..10906)
                     /locus_tag="AC16QF_06850"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019690290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR6448309.1"
                     /translation="MAYVAIADRDEHLHDPDTFITKYIWSQDHKVIAIQYGLTAIFVG
                     LVALVLSGLMRLQLGFPDQFSFIDPGAYYQYITMHGMIMVIYLLTALFLGGFGNYLIP
                     LMLGARDMVFPYMNMLSYWVYLLSVIVLMLSFFAPDGPTGAGWTLYPPQAILPGTPGV
                     DMGIVLMLGSLALFIVAFTMGGLNYITTALQARTRGMTLMRMPLSVWGIVMATVLGLL
                     AFPALLVSAIMMLLDRLLGTSFFMPAVVSLGDQLSYEGGSPILFQHLFWFFGHPEVYI
                     VALPAFGIVSDLLSTHARKNIFGYRMMVWAIIAIGGLSFIVWAHHMYVSGMHPYFGFF
                     FATTTLIIAVPTAIKVYNWVLTLWRGNIRLTTPMLFAIGFVFTFINGGLTGLFLGNVT
                     VDLPLSDTYFVVAHFHMVMGVSPILVVFGAIYHWYPKMTGRMMDETLGKMHFWITFIG
                     TYSIYYPMHYLGFLGVPRRYYAMGNAEFMPESANALNAGISVAALIVGVAQLIFLYNA
                     IVSLRKGKPAGGNPWEATTLEWQTPDTPPIHGNWRDNLPVVHRWAYDYSVPGVDQDYI
                     PQNVPHSETHPAHG"
BASE COUNT         2002 a         3318 c         3498 g         2112 t
ORIGIN      
        1 ccggatacgc tgcccagcca tcgtctggtg tactcgacga acgggagccc cgaggactgc
       61 atgtctgcca tcagcgcctg gaacgcggcg ccgctttcga tggtggtcat ccccatgtgg
      121 tcagagaagt gcagatgagc gatgacgtcg tagatcgtcc agtctttgaa ggtcgttctg
      181 cgctgccagt cttcctccgt cagcttggac atcagctcgg ccagctcggc gccttcggcg
      241 cgcaggtcgt ctacctgcct ggcaagctcg atttgttccg cggtgctcat gatcttggat
      301 cccgaagctg aggggccatc atataccagg cgtgttggcg cggctcatcc gcccggcccg
      361 gctcaggacg tcgcgtagac gtcgcaaagc gctggcccta ccatgaagca tccggcgaga
      421 ttccgattcg agggccgacg catgaccgaa cccgctgccg tccgagccga gattccagat
      481 ccgtattccc tgcctctgga cacgctcaac gtggcccagc cggcgctgtt tcaaaacgac
      541 gcccactggg gttacttcga gcggttgcgc aacgaggacc cggtgcacta ctgcccccag
      601 agcgatttcg gtcccttctg gtcggtgacg aagttcaaag acatcttcta cgtggacacc
      661 catcacgaga tcttttcatc cgagggcggg atcacgttgg gcccgcccgt caaccagcct
      721 ccggaggaag gattccgtcc gagcatgttc atcgccatgg atcctcccaa gcacgatgtg
      781 cagcgggcga ccgtgacccc cgtggttgcc ccgcgcaacc tggccaagct cgagagcgtc
      841 attcgtgagc gcgccgggcg gattctggat tctctgccgc gcggcgagac gttcgactgg
      901 gtagacacgg tttccgtcga gctgaccacg cagatgctcg cgacgctctt cgacttcccg
      961 ttcgaggaac gccgcaagct cacgcgttgg tcggacgttg cgaccgcgat accgggggca
     1021 ggcatcgtcg acagcaacgc gcagcggcgc gaggagctgc tggagtgtct ggattacttc
     1081 acgcgtttgt ggaacgagcg ggtcaactcc gagcccggga acgacctcat ctcgatgctt
     1141 gcgcacgggg aggaaacgcg ccacatgcag cccatggagt tcctgggcaa cctgatactt
     1201 ctcatcgtgg gcggcaacga caccacgcgg aactcgatca gtggcggtgt tctggcactc
     1261 aaccagaacc ccgaccagta tcgcaagctc cgggaaaatc ccggcctgat ccgcaccatg
     1321 gtcccggaga tcatccgctg gcagacgcct ctggcctaca tgcgacgtac ggccaaggtg
     1381 gatacggagc tgcgcggcaa gaagatccgc gcgggtgaca aggtggtcat gtggtacgtg
     1441 tccggtaacc gcgacgggga ggtcatcgac cggcccgacg agttcatcat cgaccggcct
     1501 aacgtccgcc atcacctagc gttcggtttc gggatccatc gctgcatggg taaccgattg
     1561 gcggaaatgc agctgcgagt cgtctgggaa gagattctgc aacgctttca caccgtcgag
     1621 gtggtcggag agcccgagcg gacctactcg tcattcgtca agggctacac gcgattgccg
     1681 gttcgtcttc acgagcattg agctagctgc cgaaatctaa cggtcccggt gaccggtcgt
     1741 tactcgatga atacggtggc cggctgctcg agatagccct tcaccgcctg aatcagagac
     1801 gccgcgtcga agccgtcgac aaagcgatga tcgaacgagg acgaaaggtt catcatgagc
     1861 cggggcacga cctcgtcatc caccaccacc gggcgcctga ccgccttgtt gacgccgatg
     1921 atggcgactt ccggcgcgtt gatgacgggc gtcgacacga tgccgccgag gcgtcccaga
     1981 ctggtgacgg tgatggtcga ccccgtcagg tcggccggcc gcgcggtgtt cgcccgagcc
     2041 gcctcggaca cccgtgccat ctccgacgcc aggtcccaaa gcgtccgctc gctggcattg
     2101 cgaaccacag ggaccttcag cccggcggaa gtctgggtgg cgacgcccag atggacccgg
     2161 tcgaaaagcc gcaacgtgcc cgactcggct gcgaaatggg cgttgcattg cgggtgtgct
     2221 ttcagagcac ggacgagtgc cagcgcgatg aacggcagat aggtcagggc agggcgaccc
     2281 tctgcccgat cagcgttcag gtgcgaccga agggattcca gggcggtgac atccacttcc
     2341 tcgacgtagg tgaagtgcgg aatttcgcgc acggctgcct gaagccgctt ggcgatgacg
     2401 cgacgcacac cgatgacctt gacctcgttt acggcacccg tgccgctcgc cgggggcgct
     2461 gctgtctccc ggccggcgat ggcctgctcg agatcgcgct gcagtatccg tccgcgcggt
     2521 cccgtgcccg tgagcgactc gagatcgacg ccggcctcct tcgcccgtcg tcggatggcg
     2581 ggggacgtga tcacgcgcct tgcgctctcc accggcgtat cgggcagcgt ggcaacttgg
     2641 tctgaatcgg ccgacgacgg ggacgagggt tctggatgag tgaccgcgtc ctcgataggt
     2701 gcctccactg gctcgggccg catggagaca tcaggggatt ccggaagggc gatggcggac
     2761 cctaccgtgc tcggggcctt cgtcgtctcc tgggaggctc cggtctcgat ggcggccagc
     2821 tcagcgccga cggcgatcaa atcgcccggc attccggacg ttctgagcac ggtcccggtg
     2881 accggcgcgg ggacctccac gctggctttc tcggtcatca cgtcaaccag aagatcgcct
     2941 tcgttcactt catcgcccgg gcgtacatgc cactcgacga tctccgcctc gaccgttccc
     3001 tcgccgagat cgggaagctt gaatacgtat tcgctcatgg cgcctccagt acccgttcca
     3061 gggcggccgc gacgcgcttc tggcccggaa aatactccca ctcgaatgca tggggatacg
     3121 gggtatccca accggcaact cgctggatgg gtgcttcgag gtggtaaaag cactcttcgc
     3181 taaccgtagc ggctagctcg gcgccgtacc cgctgaaccg cgttgcttcg tgggcgatta
     3241 cgcagcgccc cgtcttctcc accgaggtct tgagcgtttc gacatccagc ggcgccatgg
     3301 tccgcacgtc gatgatctcg gcgtcgactt cagcggcttc cgcggccgcc gcgcagacgt
     3361 gaaccatggt gccgtaacag aggatggtca cttcgctgcc gggccgtatc acctcggcgc
     3421 ggccgatggg gatcgtgtag tagtcttccg gcacctcgcc cttggggtgc gtgctccacg
     3481 gaacggccgg tttgttcgcg tcgccgtcga aggggccgtt gtagattcgt ttgggctcga
     3541 agaaaatcac cgggtcgtcg tcctcgatgg cgctgatcag gagccccttg gcatcgtaag
     3601 ggtttgaggg catgacgacc ttcagcccgc agacgtgggt gaagattccc tccggactct
     3661 gggagtgggt ctgcccgccg cgaatgccgc ctccgcaggg cgttctgatc gtcaccgggg
     3721 cgaagaagtc gccgccggtt cgataccgca ggcgcgcgag ctcgctgatg atctgatcac
     3781 aacccgggta gatgtagtcg gcaaactgaa tctcggctac cggccgtagt ccgttgacgc
     3841 ccatgccgat ggcgctgccc acgatgcctc cttcggcgat gggcgtatcg atgacgcggt
     3901 gctcgccgta ctgcctctgc agaccgtcgg tgacccggaa aacgccgccg aagaaaccga
     3961 cgtcctggcc aatgacgatc acgcccgggt ctcgactcag catgatgttc agagccgagt
     4021 ttagcgcctg gatcatgttc atctgggcca tgtcggtgac ctcctcattc ttccagcgcg
     4081 cggagctgtt cgcgctgggc gagcaggtgg gcgggaacat ccttgaaaac gtcctcgaac
     4141 atctcgctgc ggtcgagcca cggaccctcc gtcatggagc cgtagctcag ggcttcctgc
     4201 caggcctcgt cgacagcggc ctgcaactcc tctaccagct gctcgtgctg ggcctgtgac
     4261 cagtaacctt cgcgaatcat gtgctgtttc agacgctcta tgggatctcc cagcggccag
     4321 ctccgttctt cgtcgcgggg acgataccgc gacgggtcgt cgctggtgga gtgggcggat
     4381 gcccgatagg tcacgtgctc gatgagcgtc ggtcccacac cgcgtcgtgc gcgttcggcg
     4441 gcccagctcg ttaccgagtg cacggcgaga aaatcgttcc cgtctacccg gattcccgga
     4501 atgccgtaac cgggccccct ggccgagaag gggcggcgtt ccccgcccgc gaaaccctga
     4561 aaagtcgaga ttgcccattg gttgttcacg atgttgagga tgacgggggc caggtagacc
     4621 gatgcgaacg tcatcgcatg gtgaaagtcc gcctcggcgc ttgagccctc gccgatccat
     4681 gcggcggcca catcgtcgtc gcccttgatg gcggcggcca tggcccagcc cacggcctga
     4741 gggaactggg tggtcaggtt gccggaaacc gagaagatgt tccctcgggc ccagtgatac
     4801 atcacgggca tctgtcgacc tttgcacatg tcacggctgt tcgacaggca atggcacatg
     4861 agatcgacca ggctgcggcc cctggcgatt tgcaggcctt gagtgcggta ggaagggaac
     4921 agcatgtcgc tcggcctcaa ggccatcgcc ggggcgatgg cgacggcttc ctctccggtg
     4981 cacttgatgt agaacgaaat gcgaccctgc ctctgcatgc gcagcatccg gtcgtcgtac
     5041 aggcgtgtga gaagcatgtg tcgcaggccg atctgaagat cgtggtcgtc cagatgcgga
     5101 ttccaggagc cgacggcggc gtggtcgtcg tccagaacgc gtaccatctc gaaagcgagg
     5161 ctgcgctcta tgtctctgag ccgggtagtg acgtcgggcc ggggcactgc gcctgcaggg
     5221 gagatgtcga cgtagctgaa gtctggttcc tgccccggcc gagccggggg gtgcggtatg
     5281 tgcagcctgg gtttgttgct cattgtctaa ggcctccggg gcttctatcc gtgtgcatga
     5341 tgggctgtga cgtcatgatc gggtgcaggc catcgccctc ggcgatgctt tgcgctgcgc
     5401 cggcgctacc cagaatttcc gcgtcgcgat tgaccgcatg atgacgggtt acgtccgtgt
     5461 tgccccctca tgattctact actgttcagg cctggggcga ttgcctatgc taccaatggg
     5521 cggaggaggc gacttggcta aagattcaga gtcggcgggc gggtcgcgtg tccggatgtt
     5581 tcatggatgg tggatcgtcg ccgtcgcgtt ctgcgcgcag ctgctcgcgc tgggctgttc
     5641 cgttgccgtc tacgggctgt tcattcccgt gctcactgac gagttcggcg catcgttcat
     5701 gacggcaaat ctcgggttgt cgatgctcgg cgtggtgatg gcgctggccg gcgccctggt
     5761 cggtccgctg ctcgaccgtc gttccatccg ggggatcatg gttggtggcg cggcgatcag
     5821 cgggcttgcc tttctgctca tgtcgtatgc gaccgagctc tggcaactgg ccgtcctgtt
     5881 tggcgttgcc gtggccttcg gggccgccat gttcgggccg ctggccgcca acaccgtcat
     5941 tgccaagtgg ttcagccgca accgcgggcg cgcggttggg atcgccagca tgggcccgcc
     6001 cactggcggt ttgctcctct cgcccctggt cggcgtcatt atgtcatcct acggatggcg
     6061 accgactttg ctggtctttg ccgggttgca cgccatcctc ataccctttc tttggtttgc
     6121 gattcgcaac cggcccgagg atatgaatct actgcccgac ggcgctcgcg cgatgggctc
     6181 gggcgcaacc gtgggcaggg ggtggaccac ggcggacgtg tttcgatccc ggaatttctg
     6241 ggtcctagcc ctggccttcg gcgcggttgg cgccgtggcc ggggcgttca atgcgaacgt
     6301 catcccttac gcccgcgaca tcggaatagg cctcaccgaa gcctcgcttt tcatctccgc
     6361 cattggcggc acggccatcg tgggcaccgt ggtgtttgga accctggctg accggatgaa
     6421 catcacaagg ctgctgctgg tctcctttct catgcaggcg ttggctttct tcacgctgcg
     6481 ctcggtagcg cccgggtatg gtttgctcat gctcgtcgtc ctggttttcg gcctggccgc
     6541 aggggcgctc atgccgctca tggttgcggc ggtggggcgt gctttcggcc cggtgtcgtt
     6601 cgggcgggtc atgggcttca tcgggcctgt gaccctgccg ttcgctttcg tgggcccgcc
     6661 gcttacgggc tggatccgtc agacgacggg atcctacctg ccggcgttcg atctgttttt
     6721 cgccgtgttc gtggtgtgct gcggcatcgt aatgatgctc aaagtgccgc gcgccacgga
     6781 gtcagccgag gaaaacctga ggacggggtg aacggctgat gcagaagaag agggcgcggg
     6841 ggggctggcc gccgcgctcc tgctcagggc atgtcagggc atggtttcgc cgaagaaacg
     6901 ggtcaatctg gtctgccagg agatgatcca tggaggcgtg gacacgccgg tgcctgccat
     6961 ggttgcgctg gggtgggccc gaacgatctt ctggaagacg ggcacctggt cgggcgccag
     7021 atcacagtag aagacgtgct tgccttgctc gagcgtcgct ttgaattgct tgaaatggcg
     7081 attgagcgtc tgaattccga cgaaaccgcc ctcccatgtg cagaagccga ggacgatgat
     7141 ggcgaggaag atgaatggta tccagcctgc cgccgtctgg tgccaaccga gcaacgccgt
     7201 tgcgcccaga accgatgcgc tcagcaccgc accgaggatg gcgccgatga tcatcgagtt
     7261 caccacgtcc cgcttcatga tggattggac atcatgcagg tggacgtgct tttcggcctc
     7321 tgtgtcgtcg agcgtgaaga cgtgaatctg cggtgttgaa acgccctgtg cctcgagctc
     7381 ctcttcgagg tgctcgagat cgtcgagatc gtcactgatg aggaagtgcc gctgcatctt
     7441 catcttatct cccctcctaa ctggctttac tcagtcaccc tgaaagaata tgacgcgggt
     7501 cagttcggtg tagtcgcctt cgatcaccat caggccgatg agtatgagga ggcaaagtgg
     7561 tggcacgaga atggccgtga tcagtgccag cctctcccag gccatgtgca tgaaaatggc
     7621 cacgatgaac ccggccttca gaaacatgaa tatcaggatg agcgaccagc gcagcattcc
     7681 ctgaaactgc atgtagtcga ccccgtaaga aaaggcgctg aagagaaaga gcaggaacca
     7741 gatccacaga tagatcttga tcgggtgctg ctgtccctca ccgtgcgcgt cgtgagttgc
     7801 cgcttgcgta tccgccatgt tgatttccta ccagagatag aagaacgcga agatgaatac
     7861 ccagaccagg tcgacgaagt gccagtacag cccggagatc tcgaccatgt cgtagttgcc
     7921 ctttttgtcg tagaagccgt tggcgacccg cctggcaacg acaacaaggt agatcacgcc
     7981 ggcagacacg tgcagtccgt ggaagcccgt gatcatgaag aagctggagc cgaactgagc
     8041 tgcgccccag ggattacccc agggacgaac gccctctgaa atcagcttag tccactcgaa
     8101 cgcctgcatc ccgacgaagg tcgctccgaa cccagcagtg gcgagcatca gcgccacggt
     8161 ctttttcttg tcgcgtcggt atccgaagtt gacggccatg gccatggtgc cgctgctgct
     8221 gatgagcacg aaggtcatga tggcgatgag caggagcggg acgctcacgc cgaacacgtc
     8281 caggccgaat acctggctcg ggttcggcca cgggatggtc gtggagattc tcaccttcat
     8341 gtagctggtc agaaaacagg agaagatgaa ggtatcgctg agcaggaaga tccacatcat
     8401 cgccttgccc cagggcacgg gaaacgcctg cttatccgcg gaccaatccc cgacgatgcc
     8461 ctgccagcct tcgccctctc cgaaggcggg aacgccggct gctgctacgt tgtttgccat
     8521 attcgtacca ttctcaggtt gaaatcagga tccaaaggat tgccagccac accagggcca
     8581 gaaagtgcca gtacgtggtg cacagctcta cgctcagtcg gatctggtag ggctccgctc
     8641 cgttccagac cttcagagtc gttctggccc agaccaccag gccgccgatc aggtgcaggc
     8701 cgtggacggc ggtgagcagg taaaagaacg cgttggccgg gttggttgcg gcaaagtaac
     8761 cggccgagcg aagttcttcc caggccatgt actggccgac aaggaagccg atggtgagca
     8821 gcccagcggc cagcaggccc gcatttacgg cgcccgcccg gctgcgctga acggagtagc
     8881 gcgtgtactg aagcgcaacg ctgctcagca ggatgacgac ggtgttgatc cagagcagat
     8941 ccggctccgg taacggccgc cagtcgtcga gttccatgcg gatgtagtag gccacgcaga
     9001 acagcgagaa caccaccagg acgacggtca gaaagactcg cagcccgatc ttggcggagg
     9061 gcgtacccat gacgagtgcg ccggaacgca ggtcggcgac gacgtcctcc tctggtgcca
     9121 gccacgactt ttccgttatc tgcttgagaa atttcatcgc agatcaccca tgggccggat
     9181 gcgtttccga gtgcggcacg ttctgcggga tgtaatcctg atcgacgccg gggacgctgt
     9241 agtcgtaggc ccatcggtga accacgggca ggttgtcacg ccagttcccg tgaatcggcg
     9301 gggtatccgg ggtctgccat tccagcgtcg ttgcctccca gggattgccg ccggccggct
     9361 tgcccttgcg gaggctcacg atggcgttgt acaggaagat cagctgagcc acgccgacga
     9421 tgagggcggc cacggagatt cccgcgttga gcgcgttcgc cgattcgggc atgaactctg
     9481 cgttacccat ggcgtagtac ctgcgcggga cgccgaggaa gcccaggtag tgcatcgggt
     9541 agtagatgga gtacgtgccg atgaacgtga tccagaaatg catcttgccc agggtctcgt
     9601 ccatcattcg cccggtcatc ttcgggtacc agtgatagat ggcgccgaac accacgagta
     9661 tgggagagac gcccattacc atgtggaagt gcgcgaccac gaagtacgtg tcggacagcg
     9721 gcaggtccac ggtgacgttg cccaggaaca ggccggtcag ccctccgttg atgaacgtga
     9781 agacgaagcc gatggcgaac agcatgggcg tggtgaggcg aatgttgccc cgccacaacg
     9841 tcagcaccca gttgtagacc ttgatggccg tggggaccgc gataatcagc gtcgtggtgg
     9901 cgaagaagaa cccgaagtac ggatgcatgc cgctcacgta catgtggtgc gcccagacga
     9961 tgaagctcaa cccgccgatg gcgatgatcg cccacaccat catcctatag ccgaagatat
    10021 tcttgcgcgc gtgggtgctc agaaggtcgg acacgatgcc gaacgccggc agcgccacga
    10081 tgtacacctc ggggtgtccg aagaaccaga aaagatgctg gaacaggatc gggctgccgc
    10141 cttcgtagct gagctggtcg ccgagagaga cgacggccgg catgaagaag ctggtgccca
    10201 gcagacggtc cagcagcatc atgatggcgc tcacgaggag agcagggaag gcgagcagac
    10261 caagcacggt ggccatcacg attccccaaa ccgacagcgg catccgcatg agcgtcatgc
    10321 cgcgggtgcg tgcctgcaga gccgtggtga tgtagttcag accacccatt gtgaacgcca
    10381 cgatgaacag cgcgagggag ccgagcatca gcacgatgcc catgtccacg cccggcgtgc
    10441 ccggcaggat ggcctgcggc gggtaaagcg tccatcctgc tccggtcggc ccgtcggggg
    10501 cgaagaagct gagcatcagc acgatgacgg acagcaagta tacccagtag ctcagcatgt
    10561 tcatatatgg gaaaaccatg tcccgtgcgc cgagcatcag cgggatcagg tagttcccga
    10621 agccgcccag gaaaagggcg gtcagcagat agatcaccat gatcatgccg tgcatggtga
    10681 tgtactgata gtaggcgccc ggatcgatga acgagaactg gtccgggaaa cccagctgca
    10741 gccgcatcag gccggagagc accagagcca ccagccccac gaagatggcg gtgagcccgt
    10801 actggatcgc gatgaccttg tggtcctggc tccagatgta cttggtgatg aatgtatccg
    10861 gatcgtgaag gtgctcgtca cgatccgcaa ttgcgacata tgccatcaga tcctccggct
    10921 gccgttgtgc
//
LOCUS       DBNHLM010000148        16633 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_919398, whole genome shotgun sequence.
ACCESSION   DBNHLM010000148 DBNHLM010000000
VERSION     DBNHLM010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 16633)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16633)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16633
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_919398"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="AC16QF_06855"
     CDS             <1..712
                     /locus_tag="AC16QF_06855"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6448310.1"
                     /translation="DLERGVKIVKIFASGGHGAPAGMCMSAEEVKAVVEVAGDYGARV
                     RAHVAGKQQILDCLRAGVTIIDHGDEADEEVIEAYLEHDAFVLPSGYVLLKAAELGGN
                     TFGFSEDLKDFESLCRLLPRLVDAGVKLVPGDDFGIWQIPHGAYAEELVCYAEHAGIP
                     PLELIKWSTLHGGEMTGFEGIGTIAPGKVADLLILNDDPSRDIRVLTRPESVQAVLKG
                     GQLVSGELPTPLAAVSAG"
     gene            complement(782..1660)
                     /locus_tag="AC16QF_06860"
     CDS             complement(782..1660)
                     /locus_tag="AC16QF_06860"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6448311.1"
                     /translation="MSDAQARGLPPDDPTTLITPELIEQYRRDGVICVRNALHPEWLM
                     LLEMGLERIMADPGQQKHLFYRGEPGEFIETIRNFDVAPEIRRLIYDSPIADMIGKLI
                     GSGNIWLYSDEFFVKEGGGCERTPWHQDLPYWPLEGEQIASMWISLDPLPREDCLEYV
                     AGSHQGPMFDGFDPQRVTEDPTLPHYGEGLPPLPDIEADRASFNIVSWEISPGDVILA
                     HPGVLHGGGATGIADRRRAITVRCYGDDVVYATRPPTRPTVPLTPGLGLKLKPGDPLR
                     HPYYPRLRPLPEHQRV"
     gene            1831..3174
                     /locus_tag="AC16QF_06865"
     CDS             1831..3174
                     /locus_tag="AC16QF_06865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR6448312.1"
                     /translation="MDLNTLTSFEQRYLWEPDYRQAPPWQRVLVGMARTLMLLFRDIF
                     HGDISLRAMSLVYTTLLSMVPLLALSFSILKGFGVQNQLDETLRAALAPLGEEKALEL
                     TENLITFVNNMNVGVLGAVGLLFLLYTVVSLMQKIEDSFNHTWRIRTPRTFGERFTTF
                     LSAITIGPLLIFTAIALTGSIMSTTVMTHVQEIAVVGPAIQIVVRLLPFVFVIGAFTF
                     LYVFIPNTRVRISAALTGGIVAGVLWELLGFGFASYASGASRYQLIYATFATAIFFLI
                     WLYLNWLILLIGASIAFYRQHPEVIASGSKEVHFSQQLTTRYVLSLLAKIGRRHYEKK
                     KPYTLDELTASYRIPANALEHCLELLVALDVLAETDEPKPSYIPALPLDTTTVAEVLQ
                     RVETYQPPGTYAPALGADEDIVTIEAAGGSGRDRALGGLTLKQLAAGDSSEVVTA"
     gene            3313..5727
                     /locus_tag="AC16QF_06870"
     CDS             3313..5727
                     /locus_tag="AC16QF_06870"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HIR6448313.1"
                     /translation="MVMRRSLGRHRLIMASCFIWLASWPGHASAQQTTSPDAAGSQGT
                     PAEVQPTTDLTSRTQATLTSLDRISGEINELGQLADDAAELDALQIQTDELISITENI
                     DGRGADASFDGSLVKARALAVRAEAVTTALSDHALAMEAKLEEIRQTTAQWQRIQGEI
                     TDIPEAISQRIARILATGAGLENRINAKLNETVELQNEALGVLDVARSLQERFDSYGR
                     SRRGQLLEANAAPVWLMKSEHLSSSSEKAGRDFVGTLRRDYATWVETSQAIIGGHFLL
                     LPLLLFLLTKLKATALQSPTGALARPTSTGLLIWMLLGVAIYAGAPVAVRTVFVAIAS
                     IVTGFVLVGFLDKRLRAGAIAFLILNVVEDLISGFRVTDALPRLVYLLLALGAIVLVM
                     LARRPHTLSAMLAWRMPRPILVSGMWTGIALLVLSCIANVAGYVYLSQVLVNGVLAST
                     GVLLVLFAGLTSVSEIIVAVLSLDALNNVRSIANHRYRLNRALRRPLVVLAMFLWAWA
                     TTRAFGIDDWVIASIGAIVGAEMSIGSVTLSLQGVLVFVIAVLSAVWASRIVRAVLNY
                     DVLPRMQLPRGVPNTISMTLHYSIILIGLLLGVGFMGIDLSSLAFIVGALGVGIGFGL
                     QNLVNNFVSGLILIFEAPIQVGDTVEVGPLMGKVVQIGIRTSRVRTFGGSEVIVPNGD
                     LVSNQVINWTLSDRSRRLELEVGVAYGSDPERVTEVLRSVVDGDEDVLEDPAPIIMFE
                     TFGESSLNFRIFAWIDDFDKGFGMRHRLNVAINRALAEAGLEIPFPQRDLHIKNVPPE
                     LGAKPA"
     gene            5724..6212
                     /locus_tag="AC16QF_06875"
     CDS             5724..6212
                     /locus_tag="AC16QF_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448314.1"
                     /translation="MSTSEKPAEPVPYMRRSRAYYEAQGFERAYRYAHHDAAPFAPLP
                     RPLSECTVGLVTTASTYPRASLEPRRVVSSPTRPAPDKLFADDLSWDKQATHLDDLNS
                     YFPIDHLQTEAAAGFIGTLAQRFHCAPTEYSQRATSEKDAPELHRRLLQDEADVALLI
                     PL"
     gene            6267..6587
                     /locus_tag="AC16QF_06880"
     CDS             6267..6587
                     /locus_tag="AC16QF_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448315.1"
                     /translation="MPTVIIGSALDIVEHCGVPRFLFVDFPLGNPCGRPGDAAMQKRI
                     VRQGLELFETADTGQTTIYCAESWGSDEWRERYMEVTEGNRAELARKGDELRQRRADR
                     QRRP"
     gene            complement(6600..7934)
                     /locus_tag="AC16QF_06885"
     CDS             complement(6600..7934)
                     /locus_tag="AC16QF_06885"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6448316.1"
                     /translation="MSGSAKFAFTPADKVPDPDLGTGRIPVDRYTDPAFLKLEDERIW
                     SKTWLLAGAACDVAEPGEYFVFENLRESILVTRGDDGEVRAFYNVCQHRGNRLKPSGC
                     GYSEYLRCGFHGWEWNMDGSLKHIPEAHDFPQGTPADELVLQPLRCDTWGGFVWVSMD
                     PDIEPLQDYLGVIPDHLAPYEFEDMVLVSDETVEWNCNWKTSVDIFSETYHVRGVHPE
                     SEYIADDVNVQMDIYGPHSRFIAPMYIPSPRKIGEDRALNQVMVDYLKSEGFPVEDFD
                     GNVTTLRSAVAAHKRAMTNENGLDFSRLTDDQLTADYHYNIFPNVTFNLFGEQMWMFR
                     HRPHPTDPDKMYFDRMIFNRVPKGDVTATANAGAVDLFVELGDEQVEGRPEHVFYRYG
                     EKSSGLLLDQDASCLAGVQQGLHSRGMKGFWISQHEIRIRNFHHWWERYMGI"
     gene            8428..8607
                     /locus_tag="AC16QF_06890"
     CDS             8428..8607
                     /locus_tag="AC16QF_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448317.1"
                     /translation="MAGGNGLVEMERIAREVEPLFGVGIEIWGFDSGKGLPAPRDYRD
                     LPYIWQAAARVLLHG"
     gene            8612..8845
                     /locus_tag="AC16QF_06895"
     CDS             8612..8845
                     /locus_tag="AC16QF_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448318.1"
                     /translation="MSTDTGLLSERVGQRRAIHEYNSVDENREPSPIAGFRHTRAVPA
                     RWNEQVCIHHNYGHPDYCTYIHEQADRQLPLAG"
     gene            complement(8851..9309)
                     /locus_tag="AC16QF_06900"
     CDS             complement(8851..9309)
                     /locus_tag="AC16QF_06900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6448319.1"
                     /translation="MSDVKDEDRAAFFEAVDAACKKAVWCALASVAGDRPRVRIVHPT
                     WDGEVLWFATGPDSPKARQLGENPNVDIQYQVAPPDFVHVMVRGTAELVTDQAAKNHA
                     WEAIDYDLTQFGSTGPDDPNFLPVRITPTRVELSEMFGSVNKRVWRAGTP"
     gene            complement(9418..10437)
                     /locus_tag="AC16QF_06905"
     CDS             complement(9418..10437)
                     /locus_tag="AC16QF_06905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6448320.1"
                     /translation="MKDLKLGLQLGYWGAGPNPDMVKVAQEAERLGYDAIFTAEAWGS
                     DVFTPLAWIGAHTERIRLGTGIAQLSARAPTATAMAALTLDHLSKGRVILGLGVSGPQ
                     VVEGWYGRPFGKPLARTREYVSIIRQVLERKEPVTNDGEHYPLPYTGEGSWGLGKPLK
                     SITHPYRSDLPIFLGAEGPKNVAMTAEIADGWLPLYYSPYRQEVYADQIAHAKPGFEI
                     MQGVSVIINDDVEEALIPLKRNLALYIGGMGAKSRNFHNELVQRMGFADAAAKIQDLF
                     LSGRKDDAAAAVPTELADEISLVGSVERIKDRLQAWRETPVTSLLVSTQNISQLRTFA
                     EIVLG"
     gene            complement(10606..11004)
                     /locus_tag="AC16QF_06910"
     CDS             complement(10606..11004)
                     /locus_tag="AC16QF_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448321.1"
                     /translation="MKFVGYLIGSVVILVVLIFVSQLVASESAEVVVLTSQGPDGGQD
                     TRLWVVDRDGLQYLRASPDSGWYQRLAAEPQVLLVRNGNSAAYRAEIRMALAEDVNRL
                     MREKYGWRDAYIEFLIGDRMAAVPVALIPD"
     gene            11152..11889
                     /locus_tag="AC16QF_06915"
     CDS             11152..11889
                     /locus_tag="AC16QF_06915"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6448322.1"
                     /translation="MWIFYSPFHTFIHKVLVTAHECGHWDDITFVATFPFKNNEGIDQ
                     GDAYDISALNPLNKVPTLVTGEGQVIYGSQAACEYLDGTSRARRMYPEPGPARWDAVT
                     RLALSDTIFEQTVVMVMEQWKPESEWNRGLFEWLWPKFIAGLDKLERDAERGWGDFDV
                     GHAAMLHAISYLGFRAEFYEAKDPIHPDFRWRDGRPALSAWFDEAVERPSVQSHYLKD
                     FVGDSSAEFCQAQVQAVLAAQKQHDTG"
     gene            11886..12587
                     /locus_tag="AC16QF_06920"
     CDS             11886..12587
                     /locus_tag="AC16QF_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6448323.1"
                     /translation="MIDLYYWPTPNGHKVAIMLEEIGSEYQLHPVNILEGEQFDPDFL
                     EISPNNRIPAIVDRSGPDGTPYALFESGAILLYLAEKTGVLWPSSTAARYDMLQWLFF
                     QNANVGPMFGQCGHFQGYAPEPVPYAIERYHNETRRLYGVMDRRLASCAYLGGPAYSL
                     ADIATYPWCMPIIRELHKIDIDEFPNVADWADRIAQRPAVARGTSLLADVMKIGDPDE
                     KTREAFFGSRQLEQR"
     gene            complement(12599..12997)
                     /locus_tag="AC16QF_06925"
     CDS             complement(12599..12997)
                     /locus_tag="AC16QF_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR6448324.1"
                     /translation="MEPPTEPRLIKTDPDPFEPFCIAQGYQVGNLIFLSGQAALDLQG
                     NVVGSGDFDLQAEQALRNVQMLLDQAGSGLDKLIKVTIYLTDMTQFPKIVELRRKWFT
                     PPYPADTIVEVRSLALPELMIEIDAIALAG"
     gene            13032..13988
                     /locus_tag="AC16QF_06930"
     CDS             13032..13988
                     /locus_tag="AC16QF_06930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6448325.1"
                     /translation="MRAVYYERFGGSEVLTVGELPTPEPASGEVLIRIHAASVNPVDW
                     KIREGFFDGIFPHRFPIIPGWDAAGEVAGLGDGVTEMQIGSRVYAYCRKPVVHGGTYA
                     ELVTVPAGFVAPAPANLDDVETSTIPLAGLTVWQSLFEFAGLTAGQTILIHAAAGGVG
                     SLAVQLAAHAGARVFATTSAANAAYVRELGAHRIIDYRQENLGEVMGSEVPAGFDVVF
                     DTVGGEVLGESYDLVRPGGALPALNDAPDEALCAQRGIRGLRVFSEPNGQHLSRLTGL
                     IEAGALRPAQIESFPLEQAALAMDRSQAGHVRGKLALAVGQR"
     gene            complement(14031..14429)
                     /locus_tag="AC16QF_06935"
     CDS             complement(14031..14429)
                     /locus_tag="AC16QF_06935"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6448326.1"
                     /translation="MQYQSSLSRSGLETLAIESYFGNVDAKNLDAVLDCFHDNAMITV
                     QTAFTTHVGKDGIRRMFVDFMGAYKTIIHRDFTCTIDEKNGRIAACFTAELEDHDGNW
                     TTLQNTNFWRVRGDRFQEVYIYMSGENPLV"
     gene            complement(14457..14852)
                     /locus_tag="AC16QF_06940"
     CDS             complement(14457..14852)
                     /locus_tag="AC16QF_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6448327.1"
                     /translation="MADIPYEYYVDIVTKRYFHGVDKCDMDMVLDCFHEDAVLTEVTS
                     MTVHEGRDEGIRRMFEGLFDAHSRIWHGNFVHTADVDGEAICSQFSVEVTPKETGTEL
                     RYENCNRFYLDNGKFSRVFVYMSGENLLK"
     gene            complement(14886..15632)
                     /locus_tag="AC16QF_06945"
     CDS             complement(14886..15632)
                     /locus_tag="AC16QF_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009868041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIR6448328.1"
                     /translation="MSKRVKILVPIPMDAAGVANRASQLPADRIRDGYEVEFVAVSWG
                     AALGDSYYDTMLMDFSVFEAGLKAQEEGFAAICIDTVSDSGLYALRSRLDIPVFGPGQ
                     AALHAACMLGHKFSIITMWDEWFHLYTKILKEYVLEQRCASLRSIKTRPDLEELLAGK
                     EEVIFKKLEEESLRAIEEDGADVIVLGSTTMHQSHAHLESVLPVPVINPGLLCYKFCE
                     VFLELGLSHSKRAFPSPEAPNDASVLGRHH"
     gene            complement(15650..>16633)
                     /locus_tag="AC16QF_06950"
     CDS             complement(15650..>16633)
                     /locus_tag="AC16QF_06950"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6448329.1"
                     /translation="EAHPALSLNDEFADYGTWDKGNLDLSELKKPEMLSGEYAREALK
                     NGLVLEEKFGTNPYKFGMVGATDSHTALPTAEEDNFFGKSTSVEPSATRVAHPFVASE
                     LATIEGYELVAAGYQGVWATENTRAALFDAMERKETYATTGPRMIVRFFGGWDYNEND
                     LRSRAPAFAGYEKGVPMGGDLTAAPDGKSPTFMAYAVRDPVGANLDRLQIIKGWLDER
                     GKTHEKVYDVTWSGGRQLDANGKLPAVGNTVDIEAANWTNTIGASELAAVWTDPDFDA
                     QERAFYYVRVLEIPTPWWALYDKVRLGAEIPEGVELTHQERAYTSPIWYTP"
BASE COUNT         3048 a         5439 c         5123 g         3023 t
ORIGIN      
        1 cgacctcgaa cgcggtgtga agatcgtcaa gatcttcgcc agcggcggcc acggcgcgcc
       61 ggcgggcatg tgcatgagcg ccgaggaggt caaagccgtg gtggaggtcg ccggcgacta
      121 cggcgcccgg gtccgagctc acgtcgccgg caagcaacag atactggact gccttcgtgc
      181 cggcgtcacc atcatcgatc atggcgacga ggcagacgaa gaggtcattg aggcctatct
      241 ggaacacgat gcttttgtgt tgcccagcgg ctacgtgttg ctcaaggcag ccgagctggg
      301 cggcaacacg ttcggtttca gcgaggacct caaggacttt gagagcctgt gcagactgtt
      361 gccccgcctg gtcgatgccg gcgtcaagct ggtccccggc gacgacttcg gcatctggca
      421 gattccccac ggcgcttacg cggaagagct cgtctgctac gcggagcacg ccggcatccc
      481 gccgctggag ctgatcaaat ggagcacgtt gcacggtggc gagatgacgg gcttcgaggg
      541 catcgggacc atcgcaccgg gaaaggtggc cgaccttctg atcctgaacg acgatccgtc
      601 gcgggacatt cgcgtgctga cgcggcctga gagcgttcag gcagtgctga aaggcgggca
      661 gctggtgagc ggggagctgc ctaccccgct ggctgcggta tccgcaggat aggcatccgc
      721 cgcacacggg cagagcgcaa cgagcggacc cgcaggggtc gatgcgcccg ctgatccctc
      781 ctcagacccg ctggtgctcg ggaagcgggc gcagccgcgg atagtaagga tggcgcagcg
      841 ggtctcccgg tttcagcttt agaccgaggc ccggcgtgag gggcaccgtg ggccgcgtgg
      901 gcggccgggt ggcgtagacg acgtcgtccc cgtaacagcg aacggtgatc gctctgcgac
      961 gatccgcgat cccggtcgca ccgccaccat gcagaacgcc cggatgcgcg aggatcacat
     1021 ccccgggact gatttcccag gagacgatgt tgaagctggc tcgatccgcc tcgatgtccg
     1081 gcagcggggg cagcccctcg ccgtaatgcg gaagggtcgg gtcctccgtt acccgctggg
     1141 ggtcgaaacc atcgaacatg gggccctggt gggagcccgc cacatactcc aggcaatctt
     1201 cccggggcag cggatccaga gagatccaca tcgaggcgat ctgctctcct tccagcggcc
     1261 aatacggcag atcctgatgc cacggcgtcc gctcacaccc gccgccttcc ttcacgaaga
     1321 actcgtccga gtaaagccag atgtttccgg aaccgatgag cttgccgatc atgtcggcaa
     1381 tgggcgagtc gtatatgagc cgccggatct cgggcgccac gtcgaagttg cgtatggttt
     1441 cgatgaattc cccaggctcg ccccgataga acagatgctt ctgctggccg gggtcggcca
     1501 tgatgcgctc gagacccatc tccagcagca tcagccactc cggatgaagg gcgtttcgaa
     1561 cgcagatgac gccatcgcgc cgatactgct cgatcagctc cggcgtgatc agcgtggtgg
     1621 gatcgtccgg cggtagcccg cgagcctgcg cgtctgacat tctccctctc cagaaaccct
     1681 ttgccctaat ctagcccagc ccggcaaacc tcgctacggc taagcagcgc gcggcgcggc
     1741 cgcgtcgtat gggcgcaccc gcctgggcga ggggccttga cggcacccgg gaaatccctt
     1801 tcccttcact ccacgggcag ggggacgcac ttggatctca acactctcac atcgttcgag
     1861 cagcgctatc tctgggagcc ggactatcgg caggctcccc catggcagcg cgtgctggtg
     1921 ggcatggccc gaacgctcat gctcctgttc cgggacatct ttcacggtga catcagcctg
     1981 agagccatga gcctcgtgta cacgacgctg ctctccatgg tgccgctgct cgcgctgagc
     2041 ttctcgattc tcaagggatt cggggtccag aatcagctcg acgagacttt acgtgcagcg
     2101 ctggcgcctc tgggcgagga aaaggccctc gagctgacgg aaaacctgat caccttcgtc
     2161 aacaacatga acgtgggcgt gctgggcgcg gtcggtctgc tgtttctgct ctacaccgtg
     2221 gtttcgctga tgcagaagat cgaggattca ttcaatcaca cctggcgcat tcgcacgccc
     2281 agaacgttcg gggaacgttt caccaccttc ctctccgcca tcaccatcgg gccgctgctc
     2341 atctttactg ccatcgccct gacgggctcg atcatgagca ccaccgtgat gacccacgtc
     2401 caggagatcg ccgtggtcgg cccggccatc cagattgtcg tcaggcttct gccgttcgtc
     2461 ttcgtcatcg gcgcgttcac cttcctgtac gtcttcatcc ccaatacgag agtgcgaatc
     2521 tcggcggcat taactggcgg catcgtcgcc ggcgtcctct gggagctcct gggcttcgga
     2581 tttgcctcct acgccagcgg cgcctcccgc taccagctga tctacgccac gtttgctacg
     2641 gccatattct ttctcatctg gctgtatctg aactggctga ttcttctcat aggagcaagc
     2701 atcgccttct atcggcagca tccggaggtc atcgcctcgg gctcgaagga ggtgcacttc
     2761 agccagcagc tgaccacccg ctacgtgcta tcgctgctcg ccaagatcgg tcgccggcat
     2821 tacgagaaga agaagcccta taccctcgac gagctgacgg cgagctatcg catcccagcc
     2881 aatgccctgg agcactgcct cgagcttctc gtggcgctgg acgtgctcgc ggagacggac
     2941 gagcccaagc cttcctacat tcccgccctt cccctggaca ccaccaccgt cgccgaggtg
     3001 cttcagcggg tggagacata ccagccaccc ggaacctacg cgcccgccct gggcgctgac
     3061 gaggacatcg tgacgatcga ggcagccggc ggttccggca gagatcgtgc gctcggcggg
     3121 ctcacgctca agcagctggc agccggcgat tcgagcgaag tcgtcacggc ctgatcagga
     3181 tcctcccgcc ctggaaggag cccccgcgcg atcgcgattg gcccgtggat cggctcgtca
     3241 acgtgcgcgc ctgggtaccg gttgagacca ggaagcttcg cccctaaact tcgccacttt
     3301 ccccaaaggg ccatggtgat gcgtcgatcg ctgggtcgcc acagattgat catggcgtcg
     3361 tgcttcatct ggctcgctag ttggcccggc cacgcgtcag cgcagcagac cacaagcccg
     3421 gatgccgccg gatcccaggg gacgcccgcg gaagtccagc caaccacgga cctcaccagc
     3481 cgcacccagg caacgttgac gtccctggat cgcatctcag gagaaatcaa cgagctgggc
     3541 cagctcgccg acgacgccgc agagctggac gccctgcaga ttcagaccga tgagctgatc
     3601 agcatcaccg aaaacatcga cgggagaggc gcagacgcct cgttcgacgg ttcgctggtg
     3661 aaagcccgag cgctcgccgt cagagcagag gcggtaacca ccgccctgtc ggaccacgct
     3721 ctcgccatgg aagccaaact cgaagagatc cgccagacga cggcccagtg gcagcggata
     3781 cagggcgaga tcacggacat tccggaagcc atcagccagc gtatcgcgag gatcctggcg
     3841 accggtgcag gcctggagaa ccggataaac gccaagctga acgaaaccgt ggagctccag
     3901 aacgaggcgc tcggggtgct cgacgtcgcc cggtcgctgc aggaacgctt cgacagctac
     3961 gggcggtccc ggcgcgggca gctgctggaa gcgaatgcgg ccccggtctg gctgatgaaa
     4021 tccgaacatc tcagcagctc ttcggagaaa gcgggtcggg acttcgtcgg cacgctgcgt
     4081 cgcgactacg caacctgggt agaaacctcc caggcgatca tcggcggtca cttcctcctg
     4141 cttccactgc tgctgttcct gcttacgaag ctcaaggcga cggcactgca atccccgacg
     4201 ggcgctctgg cccgccccac gtcgaccggg ctcctcatct ggatgctttt gggcgtggcg
     4261 atctacgccg gggcgccagt cgccgtgcgt accgtcttcg tggcgattgc ctcgatcgtg
     4321 accgggttcg tcctcgtcgg tttcctcgac aaaagactgc gagccggggc gatagcattc
     4381 ctgatactga acgtggtgga ggatctgatc agcggcttcc gcgtgaccga cgcgctgcca
     4441 cgtctcgtct acctgctcct tgccttgggc gccatcgttc tcgtgatgct ggcccgacga
     4501 ccccatacgt tgagcgccat gctcgcctgg cgcatgcccc gtccgatcct ggtcagcggg
     4561 atgtggacgg gcatcgccct tctggtgctc agctgcatcg ccaacgtcgc cgggtacgtg
     4621 tacttgtcgc aggtcctggt caacggcgtc ctggccagca ccggggtgct gctggtgctg
     4681 ttcgcggggc tcacgtcggt ctccgagatc atcgttgccg tgctgagcct ggacgcactg
     4741 aacaacgtac ggtcaatcgc caatcaccgc tatcgcctca accgggcgtt gcgcagaccg
     4801 ctcgttgtgc tggccatgtt cctgtgggca tgggcaacga cccgcgcgtt cggcatcgat
     4861 gactgggtca tcgcctccat cggcgcgatc gtcggcgccg agatgtcgat cggctcggtt
     4921 acgctgtcat tgcagggggt gctggtcttc gtcatcgcgg tgctgtccgc cgtctgggct
     4981 tcgcggatcg tccgggccgt tctcaactat gacgttctcc cccgaatgca gctgccccgc
     5041 ggcgttccca acaccatatc gatgacgctg cactactcga tcatcctgat cggcctgctg
     5101 ctcggcgtgg gcttcatggg catcgacctc agcagcctgg cattcatcgt gggcgcgctc
     5161 ggcgtgggta tcggcttcgg cctgcagaac ctggtcaaca acttcgtctc gggcctgatc
     5221 ctgatcttcg aagcccccat acaggtgggc gatacggtgg aagtcggtcc gctgatgggg
     5281 aaagtggtgc agataggcat ccggacgagc agggttcgca ccttcggagg ttcggaggtg
     5341 atcgtgccca acggcgacct cgtctccaac caggtcatca actggacgct ctcggaccgc
     5401 agcagacgtc tggaactcga ggtcggcgtg gcctacggca gcgacccgga acgggtcacc
     5461 gaagtgttgc gcagcgtcgt ggacggcgac gaggacgtcc tcgaagaccc cgcaccgatc
     5521 atcatgttcg agacctttgg agaaagctcg ctcaacttca ggatcttcgc gtggatcgac
     5581 gacttcgaca aaggattcgg tatgcggcat cgcctgaacg tcgccatcaa ccgggctctt
     5641 gccgaggcag gcctcgagat tccattcccg cagcgcgatc tgcacataaa gaacgtgccg
     5701 ccggagctcg gcgccaagcc ggcatgagca ccagtgaaaa gccggcagag ccggtgccct
     5761 acatgcgacg ctcccgggca tactacgaag cccagggttt cgagcgcgcc taccgctacg
     5821 cccaccacga cgcggcaccc ttcgcccctc tgcccaggcc gctgagcgaa tgcaccgtcg
     5881 ggctggtcac gacggcaagc acgtatccac gcgccagcct ggagccgcgc agggtggtct
     5941 cgagcccgac caggcccgca ccggacaagc tgttcgctga cgacctgtcc tgggacaagc
     6001 aggcgaccca tctcgatgat ctgaattcgt actttcccat cgaccatctg cagaccgagg
     6061 cagccgcggg cttcatcggc acgctggcgc aacgctttca ctgtgctccg accgagtaca
     6121 gccagcgcgc cacttcagag aaggacgcgc ccgagctgca tcgccgcctc ctccaagacg
     6181 aggccgacgt tgccctcctg atccccttgt gacccgtctg ccaccagacc gtgagtctgg
     6241 ttgcgaggta cttagaggag caaggcatgc ccacggtgat catcggctcc gcgctggaca
     6301 tcgtggagca ctgcggcgtg ccgagattcc tgttcgtcga ctttcccctg ggtaatccct
     6361 gcggcaggcc gggggatgcg gcgatgcaga aacgcatcgt caggcagggt ctcgagctgt
     6421 tcgagacggc agacaccggc cagaccacca tatactgcgc agagagctgg ggcagcgacg
     6481 agtggcgcga gcgctacatg gaagtcaccg aagggaaccg cgcagagctc gcacgcaagg
     6541 gagacgagct gcggcagcgc cgagccgatc gtcaacgccg cccataggca gcactggctt
     6601 cagataccca tgtacctttc ccaccaatgg tggaagttac ggatgcgtat ctcgtgctgc
     6661 gagatccaga aacccttcat gcctcgcgag tgaagcccct gctgaacccc cgccaggcag
     6721 gaagcgtcct ggtccagcag aaggccggac gatttctcgc cgtaccggta gaagacgtgt
     6781 tccggacgac cctcaacctg ctcgtcacca agctccacga aaagatcgac ggcgcccgcg
     6841 ttggccgtcg cggtgacgtc gcccttcggc acccggttga agatcatcct gtcgaaatac
     6901 atcttgtccg gatccgtggg atgcggccgg tgccggaaca tccacatctg ctcgccgaaa
     6961 agattgaagg tgacgttggg aaagatgttg tagtggtagt cggcggtcag ctgatcgtcc
     7021 gtcagcctgg aaaagtccag cccgttctcg ttggtcatcg cgcgcttgtg agcagccacc
     7081 gcactgcgca acgtggtgac gttgccgtcg aagtcctcca ccggaaaccc ttcggacttc
     7141 agatagtcga ccatgacctg gttcagggcc ctgtcttcgc cgatcttgcg agggctgggt
     7201 atgtacatgg gcgcgatgaa ccgggaatgg ggcccgtaga tgtccatctg cacgttcacg
     7261 tcgtctgcga tgtactctga ttccgggtgc acgccgcgta cgtgataggt ctccgagaag
     7321 atgtccacgg acgtcttcca gttgcagttc cactccacgg tctcatcaga caccaggacc
     7381 atgtcctcga actcgtacgg agccagatga tcaggaatga cccccagata gtcctgcaaa
     7441 ggctcgatgt ccgggtccat tgatacccag acaaaccctc cccacgtatc gcaacgcagg
     7501 ggttgcagca ccagctcgtc ggccggggtg ccctgaggga agtcgtgcgc ctcgggaatg
     7561 tgcttgaggc ttccgtccat gttccattcc cagccgtgaa agccgcagcg cagatactcg
     7621 gagtaaccgc acccagaagg cttgagccgg ttcccgcgat gctgacagac gttgtagaaa
     7681 gcgcggacct ccccatcatc cccgcgggtg acgagaatgg actcccgcag gttctcgaaa
     7741 acgaagtact cgcccggctc tgccacgtcg catgccgcac ccgccagaag ccaggtcttc
     7801 gaccagatgc gctcgtcttc gagcttgagg aaagcagggt ccgtgtatcg atcgaccgga
     7861 atccgtccgg tccccagatc cgggtccggc actttgtctg cgggggtaaa ggcaaattta
     7921 gccgatccac tcatgatgtc gtctccgttg agccaggggg atggagacca agacgcaagc
     7981 agccccagcc aaccgccgtg atccggaccc ctggcaaccc aaaagccatc aggtaattag
     8041 gaggaacgat actggataat cggagcttca atccagctgc ctgggctcaa cccatgggtc
     8101 gagagatcag gccctctagc gacgccgcgc agtcagcagg attggcggca caaactgccg
     8161 cagccaccac cacgcacttc tacaacgcgc tgttctccta tttctatgtc ctgatcgggc
     8221 tcggcgcttg gctcatggcg ccacagcctc acacggcgcg ccggacaaga tgggtcgtgc
     8281 tgagccctca gcaaccactg agcagcagct gacgagaatg gaacggaacc tgcccgccta
     8341 tctcaaacaa ccgaggaaac ctcaccatgt tagaccgcgt ggtaccacat ctcttgaatc
     8401 cgaccagagc catccagcga gcctggcgtg gcgggcggca acgggctggt cgagatggaa
     8461 cgcattgcca gggaagtgga accgcttttc ggcgtcggca tcgagatatg gggcttcgac
     8521 agcggcaaag ggctaccggc accgagagac taccgggatc ttccctacat ctggcaggct
     8581 gccgcgcgcg ttctgctaca tggatgacat catgagcacc gacacgggcc tcctttcaga
     8641 acgggtcggg caacggcgcg cgattcacga atacaattcg gtcgacgaga accgggagcc
     8701 ttcccccatc gccggtttcc gccacacccg tgccgtgcct gcccgctgga acgagcaggt
     8761 ctgcatccat cacaactatg ggcatccgga ctactgcacc tacatccacg aacaggcgga
     8821 ccggcaactg ccccttgccg gctgaggcgt ctacggtgtg cccgcgcgcc agacccgctt
     8881 gttgacggac ccgaacatct cagagagttc tacgcgggtg ggcgtaatac gcaccggcag
     8941 gaaattggga tcatcgggac ccgtggagcc gaactgtgtc agatcgtagt cgatggcctc
     9001 ccaggcatga ttcttggccg cctgatcggt gaccagctct gccgttccgc gcaccatcac
     9061 gtgcacgaaa tccggcggcg ccacctggta ctgaatgtct acgttcgggt tctcacccag
     9121 ctgtctggct ttgggtgagt cgggaccggt ggcgaaccag agcacctccc catcccaggt
     9181 tggatggacg atgcgcactc tcggccgatc gccggccacc gatgcaagcg cgcaccacac
     9241 cgccttcttg caggcggcat cgacggcctc gaaaaaggcc gctctgtctt catccttaac
     9301 gtcgctcatc gagctgatcc cccatttgtt gtcagcgaag gtgttgtcag cgaggggctg
     9361 gacccaacgt tggtctcctg ccacgcacct gagattcggc cggcgagctt cacccgctca
     9421 accgagcacg atttcagcaa acgttctcag ctgcgagatg ttctgggtgg agaccagcag
     9481 cgaggtcacc ggcgtctccc gccatgcctg cagccggtcc ttgatccgct cgacggatcc
     9541 caccagggat atctcgtcgg caagctccgt cggaactgcc gccgcggcgt catccttgcg
     9601 accggagagg aaaagatcct ggatcttggc cgccgcgtcg gcgaagccca tgcgttgcac
     9661 cagctcgttg tggaagtttc tggacttcgc gcccatgccg ccgatgtaca gcgccaggtt
     9721 acgcttcagc gggatcaacg cttcctcgac gtcgtcgttg atgatcaccg agacgccctg
     9781 catgatctcg aagccgggct tggcatgggc gatctggtcg gcgtagacct cctgccgata
     9841 gggcgagtag tagaggggca gccagccgtc ggcgatctcc gcggtcatgg ccacgttctt
     9901 cggcccctct gcgccaagaa agatcggcag atcggatcgg taggggtggg tgatggactt
     9961 cagcggcttg ccaagtcccc acgagccctc acccgtgtac ggtaaaggat agtgctcccc
    10021 gtcgttggtc accggctcct tgcgctcgag cacctggcgg atgatgctca cgtactcgcg
    10081 ggtacgcgcg aggggctttc cgaacgggcg cccgtaccag ccctccacga cctgcggtcc
    10141 cgacaccccg agcccgagaa tcacgcgccc cttggaaagg tggtcgaggg tcagggcggc
    10201 catcgccgtg gcggtgggcg cccgggcgga aagctgagcg atgccggtcc cgagcctgat
    10261 ccgctccgtg tgagccccta tccaggccag cggcgtgaag acgtccgagc cccacgcctc
    10321 agccgtgaaa atcgcatcgt agcccagacg ctctgcttcc tgagcgacct tcaccatgtc
    10381 ggggttgggt ccagcgcccc agtaacccaa ttgcaggccc agtttcagat ccttcacctc
    10441 aaatgctcct tcgcttggct gaactcacgg ccgcgtgacg acctcactta ctgccgcatc
    10501 tcactacgcg ggaagcaaaa gctcaagcct caatcgctcc gcggcaaggt tcccaggaca
    10561 tgcagagaac tcaaagccgg ccccgtgcct acgcggggcc ggcgatcagt cgggaatgag
    10621 cgcgaccggc accgctgcca tccgatcgcc gatgagaaac tcgatgtacg cgtcccgcca
    10681 gccgtacttt tcccgcatca gacgattgac gtcctcggcg agggccattc tgatctcggc
    10741 acggtaagcg gcgctgtttc cgttgcggac gagcagcacc tgcggttccg ccgccaggcg
    10801 ctggtaccag ccggagtcag ggctggcgcg caggtactgc agcccgtccc gatcgaccac
    10861 ccacaaccgg gtgtcctgcc cgccgtcggg cccctggctg gtcaacacaa ccacctccgc
    10921 cgattcggac gcgacgagct gggagacgaa gatcagaacg accaggatga ccaccgagcc
    10981 gatcaggtac ccaacgaatt tcacccctgc cctcctcgcg gcgccaggtg tggccggaaa
    11041 ggctaacgga tcgctgccgc gaaatgaact gctgcgccag gctgaacgtt cgggcatcat
    11101 cgccccagtg acgtgagggt cggtccggta gcgaagaggg ggaacctgct gatgtggata
    11161 ttctattcac ccttccacac tttcattcac aaggtgctgg tcaccgctca cgagtgcggc
    11221 cactgggacg acatcacctt cgtcgccacg tttcccttca agaacaacga gggcatcgac
    11281 cagggcgacg cctacgacat ctcagcactc aaccctctga acaaagtgcc gaccctggtc
    11341 accggggaag gccaggtcat ctacggcagc caggcggcct gcgaatatct cgacggcacc
    11401 agcagggccc gccgcatgta ccccgagccc ggcccggcgc gatgggacgc ggtgacgcgc
    11461 ctggcgctca gcgacaccat cttcgagcag acggtggtca tggtgatgga gcagtggaag
    11521 ccggaaagcg agtggaaccg cggccttttc gagtggctgt ggcccaagtt catcgccggg
    11581 ctggacaagc tggagcggga cgccgagcgg ggctggggcg atttcgacgt cggccacgcg
    11641 gctatgcttc atgcgatcag ctatctggga ttcagggccg aattctacga ggccaaggat
    11701 ccgatccatc ccgatttcag gtggcgcgac ggccgaccgg cgctgtccgc ctggttcgac
    11761 gaagccgttg agcgtccctc ggtccagtcc cactacctga aggatttcgt cggcgactcg
    11821 agcgccgagt tctgccaggc acaggtgcag gccgtgctgg cagcgcagaa acagcacgac
    11881 acgggatgat cgacctctac tactggccga cgcccaacgg ccacaaggtc gccatcatgc
    11941 tcgaggagat cggcagcgag taccagctgc acccggtcaa catcctcgag ggggagcagt
    12001 tcgatcccga cttcctcgag atcagcccca acaaccgcat ccccgccatc gtggaccgca
    12061 gcggacccga cgggacgccc tacgcgctgt tcgaatccgg cgcgattctg ctctacctgg
    12121 cggagaagac gggcgtcctg tggccgtcga gcacggcggc gaggtacgac atgctgcagt
    12181 ggttgttttt ccagaatgcc aacgtcggcc ccatgttcgg gcagtgcggc cacttccagg
    12241 gctacgcgcc cgaaccggtg ccctacgcca tcgagcgcta tcacaacgaa acccgacggc
    12301 tctacggcgt gatggaccgg cgcctggcga gctgcgccta cctcgggggg ccggcctact
    12361 cgctggcaga catcgccacc tatccctggt gcatgccgat catccgggag ctgcacaaga
    12421 tcgacatcga tgagtttccc aacgtggccg actgggcaga ccgcattgcg cagcgcccgg
    12481 ccgtggcgcg gggcacgtct ctgctcgcag atgtcatgaa gatcggcgac ccggacgaga
    12541 aaacgcgcga agctttcttc ggatcccgac agctggagca acgctgaggg gaagcgcctc
    12601 agccggccag ggcgatggcg tcgatttcga tcatcagctc cggcagggcc agcgaacgga
    12661 cctcgacgat ggtgtccgcc gggtacggcg gcgtgaacca ctttcgccgc agctccacga
    12721 tcttcgggaa ctgcgtcatg tcggtcagat agatggtcac cttgatcagc ttgtcgaggc
    12781 cggagcctgc ctgatcgagg agcatctgaa cgttgcgcag cgcctgctcg gcctgcaggt
    12841 cgaagtcgcc ggaaccgacg acgttcccct ggagatccag cgccgcctgg ccggacagga
    12901 aaatcaggtt gcccacctga taaccttggg cgatgcagaa cggctcgaac gggtcaggat
    12961 ccgtcttgat caggcggggt tcggtgggcg gctccatgag ggctccattg gttcgaagag
    13021 gggcagattg aatgcgtgca gtctactacg agcggtttgg cgggtccgag gttctcacgg
    13081 tcggcgagtt gccgacaccc gagcccgcct cgggagaggt gctcatccgg attcacgcgg
    13141 caagcgtcaa cccggtggac tggaagattc gcgagggctt cttcgacgga atctttcccc
    13201 accggtttcc catcatcccg ggctgggatg ccgccggaga ggtcgccggt ctgggggacg
    13261 gggtaactga aatgcagatc ggcagccggg tgtacgccta ctgccgcaag cccgtggtcc
    13321 acggcggcac gtatgccgag ctcgtcacgg tgccggctgg cttcgtcgcg cccgcgcccg
    13381 caaacctcga tgacgttgag acctccacca tcccgctggc gggcctgacc gtctggcaat
    13441 cgctcttcga gttcgcgggg ctgaccgctg gccagaccat cctgatccac gccgcagccg
    13501 gcggcgtggg cagcctcgcg gtgcagctgg ccgcccatgc gggcgcgcgg gttttcgcca
    13561 ccaccagcgc ggcaaacgca gcctatgtcc gcgagctcgg cgcacaccgg atcatcgact
    13621 accggcagga aaatctgggc gaggtcatgg ggtccgaagt gccagcgggc ttcgatgtgg
    13681 tcttcgacac cgttggcggc gaggtcctgg gcgagagcta cgacctggtc aggcccggcg
    13741 gcgccttgcc cgcgctgaac gacgcgcccg acgaggccct atgcgcccag cgcggcatac
    13801 gcgggctgcg cgtcttctcg gagccgaacg gccagcacct cagccgcctg acggggctca
    13861 tcgaagccgg cgccctgcgg ccggctcaga ttgaatcgtt tcccctcgag caagcagcac
    13921 tcgccatgga tcgctctcag gccgggcacg tgcgcggaaa gctcgcgctc gccgtcgggc
    13981 aacggtagct tggctcttcc gccgtcgatg ggctcccggg cgccaggggc tcacaccagc
    14041 ggattctccc cgctcatgta gatgtagacc tcctggaacc tgtcgccgcg gacacgccag
    14101 aagttggtgt tctgcagggt ggtccagttg ccgtcgtgat cctccagctc cgccgtgaag
    14161 caggcggcga tccggccgtt cttctcgtcg atggtgcagg tgaaatcccg atggatgatg
    14221 gttttgtagg ctcccatgaa atccacgaac atccggcgga tcccatcctt gccgacgtgg
    14281 gtggtgaacg cggtctgcac ggtgatcatg gcgttgtcgt gaaaacaatc gagcacggca
    14341 tccaggttct tggcgtccac gttgccgaag tagctctcga tcgccagggt ctcgagcccc
    14401 gaccggctca gcgaagactg atattgcact gggttatcct cctttcccgc cccggctcac
    14461 ttgagcaggt tctcgccgct catgtaaacg aacactctgg agaacttgcc gttgtccaga
    14521 tagaagcggt tgcagttctc gtagcgcagc tccgttccgg tctctttggg ggtgacctcc
    14581 acggagaact gggagcagat ggcttcgccg tcgacgtccg ccgtgtggac gaaattaccg
    14641 tgccagatgc ggctgtgggc gtcgaagagg ccctcgaaca tcctgcggat accctcgtcc
    14701 cgcccttcgt ggaccgtcat cgaggtcact tcggtgagca ccgcgtcctc gtgaaagcag
    14761 tccagcacca tatccatgtc gcatttgtcg acaccatgaa agtatcgctt ggtcacgata
    14821 tcgacgtagt actcgtaagg aatgtcggcc atggggttca cctcccttgg ttttaccgag
    14881 ctcgttcagt ggtgccggcc aagcaccgag gcgtcgttgg gcgcctccgg cgacggaaag
    14941 gcccgcttgc tgtggctgag gccgagctcc agaaagacct cgcagaactt atagcagagc
    15001 aggccggggt tgatcaccgg caccggcagc acgctttcga ggtgtgcgtg agactgatgc
    15061 atggtcgtgg atccaagcac gatcacgtcg gcgccgtcct cctcgatggc ccgcaggctc
    15121 tcctcctcca gcttcttgaa gatcacttcc tccttcccgg ccagaagctc ctccagatcg
    15181 ggacgggtct ttatcgaacg cagcgaggca cacctctgct cgagcacgta ctccttgagg
    15241 atcttcgtgt agagatgaaa ccactcgtcc cacatcgtga tgatcgagaa cttgtggccc
    15301 agcatgcagg ccgcatgaag cgccgcctgg ccggggccaa agaccggaat gtcgagccgc
    15361 gagcgcagcg cataaaggcc ggagtcgctc accgtatcga tgcagatggc ggcgaagccc
    15421 tcctcctgag ccttcaggcc cgcctcgaac acgctgaaat ccatgagcat cgtgtcgtag
    15481 taggaatccc ctaacgccgc gccccagctg accgccacaa actccacctc gtaaccatcg
    15541 cgaatgcgat cggccggcag ctgcgatgcc cggttggcaa cgcccgccgc gtccatcggt
    15601 atcggcacca gaatcttgac ccgcttactc atgctcgact ccttctggct cagggcgtgt
    15661 accagatggg cgaggtatac gcccgctcct ggtgcgtcag ctccacgccc tcgggaattt
    15721 cggcgccgag gcgcaccttg tcgtaaagcg cccaccacgg cgtagggatc tccagcaccc
    15781 gcacgtaata gaaggcgcgc tcctgcgcat cgaagtcagg gtccgtccag acggcggcca
    15841 gctccgaggc gccgatggtg ttggtccagt tggccgcttc gatgtccacc gtgtttccca
    15901 cggcgggaag cttcccgttg gcatccagct gacggccgcc ggaccaggtg acgtcgtaga
    15961 ccttctcgtg ggtcttgccg cgctcgtcga gccagccctt gatgatctgc aggcgatcga
    16021 ggttggcccc cacgggatcc cggaccgcgt aggccatgaa agttggcgac ttgccgtccg
    16081 gcgccgctgt cagatccccc cccatgggca cgcctttctc gtagccggca aaggcgggcg
    16141 cccggctgcg caggtcgttc tcgttgtagt cccagccacc gaagaagcgc acgatcatgc
    16201 ggggacccgt ggtggcgtag gtctcttttc gctccatggc gtcgaacagc gcggcgcgcg
    16261 tattctcggt tgcccacaca ccctggtagc cggcggcgac cagctcgtag ccctcgatgg
    16321 tggccagctc cgacgctacg aaaggatggg cgacacgagt cgccgacggc tcgacgctgg
    16381 tcgacttgcc gaagaagttg tcctcctccg ccgtgggaag ggcggtatgg ctgtcggtgg
    16441 ccccgaccat gccgaacttg taagggttgg tgccgaattt ctcctcgagc accagcccgt
    16501 tcttcaacgc ctcgcgagca tactcgccgc tcagcatttc aggctttttg agttcggaca
    16561 gatcgaggtt gcccttgtcc catgtgccgt agtcggcgaa ctcatcgttg agcgagaggg
    16621 ccgggtgcgc ttc
//
LOCUS       DBNHLM010000149         7229 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1219396, whole genome shotgun sequence.
ACCESSION   DBNHLM010000149 DBNHLM010000000
VERSION     DBNHLM010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7229)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7229)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7229
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1219396"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="AC16QF_06955"
     CDS             complement(<1..479)
                     /locus_tag="AC16QF_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448330.1"
                     /translation="MAADVNSWWPRAILIGAVIAAVLLVAGALGTRIGLWDFQVGFLG
                     LAGGTILAAIGVIVGAVGLIASVKRELPQNRPPIYLGLAISVVILIVMGMQFDKAYSV
                     PPIHNISTDLEDPPAFDQVVALRGEESNPLALNAQEIGPMQREAYPWVVPLVTPTAR"
     gene            complement(519..1298)
                     /locus_tag="AC16QF_06960"
     CDS             complement(519..1298)
                     /locus_tag="AC16QF_06960"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448331.1"
                     /translation="MASDLFDMTGKVALITGSSKGIGRAIAEEMARAGAQVVISSRKA
                     DLCDEVAAAINAELASEAGGAVAIPAHIGHKEELERLVTQTREKLGRIDVLVCNAAVN
                     PYFGSMTELPDSAFDKILDVNIKSNHWLIQMVLPEMLEREDGSIIVVSSVGGLRGSTH
                     LGAYAISKAADAQLVRNLAAEHGPRNIRVNTISPGLVRTDFARALWENPDILKARTSG
                     DPLRRIGEPREIAGIAVYLASRAGAFTTGQNFVIDGGATIV"
     gene            complement(1302..2336)
                     /locus_tag="AC16QF_06965"
     CDS             complement(1302..2336)
                     /locus_tag="AC16QF_06965"
                     /inference="COORDINATES: protein motif:HMM:NF048962.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7064 domain-containing protein"
                     /protein_id="HIR6448332.1"
                     /translation="MADQPGVRMRLEPADEYLHAVEEAQNFNERMYFNVFDRERRTGG
                     WFRLANRPNEGRGEMSCCVYLPDGRIGFMFKRPERHDNEAMDGGGMAFNVVEPFKHLT
                     VRYQGKLCVLADPQQMADPANAFKTNPVVESEIELDFHGISPMFGGEPVNEDGSAIEM
                     KAEESFARGHTEQHTRGAGFIRVGDERFEIDGLGLRDHSWGPRFWQAIYWYRWLPMVF
                     SEDFAMMVSITCRPDAEPRAGGMVLRDNEYVMIRDARIETVYDANDCQETFKVWARTD
                     EREYEVEGKVMSLIPLRNRRRTPDGEDLMTRITEGMTEYRCDGQVGYGLSEYLDQVVD
                     GAPVGVKAGM"
     gene            2514..3875
                     /locus_tag="AC16QF_06970"
     CDS             2514..3875
                     /locus_tag="AC16QF_06970"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HIR6448333.1"
                     /translation="MPAKSMEELVSLCKRRGFIFQSSDIYGGLQGLYDYGPLGVELKN
                     NLKAAWWRAMVYERDDVEGLDAAILTNPLTLRYSGHEETFTDPLVDCRNCKTRHRADH
                     LEGGTCPACGSSDLTDPRPFNLMFKTSVGPVDDGSSFAYLRPETAQAIFTNFKHVLDS
                     TSRRLPFGIAQMGKAFRNEITPRNFIFRVREFEQMELEFFVKPGTDEEWHHKWVEERL
                     DWWAAQGVEPNSLQLYHVPADELAHYSKATVDVMYRFPHGLEELEGIANRTDFDLGSH
                     TKAQGDFDIQARVPRNRDSNAKLAIQDLETNKWVVPYVIEPSAGVERGVLAVLNEAYR
                     VEELDDGKTRTVLGFKPHLAPIKAAVLPLKRNHEGIVTKAKAIKQQLQALGLGRMLYE
                     DTGNIGKGYRRHDEVGTPICITVDFQTIEDDDTVTVRDRDTMEQQRIAVAELPAYVTD
                     YLR"
     gene            3972..5039
                     /gene="rfbB"
                     /locus_tag="AC16QF_06975"
     CDS             3972..5039
                     /gene="rfbB"
                     /locus_tag="AC16QF_06975"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR6448334.1"
                     /translation="MKILVTGGAGFIGSAVVRHLIGATDAEVVNIDKLTYAGNLESLA
                     DAADSERYAFEQADICDAAALDRIFDQHRPDAVMHLAAESHVDRSIDGPGDFIRTNLV
                     GTYELLQASRRYLDAEARAADDFRFLHVSTDEVFGDLADTGAFFTETTSYAPSSPYAA
                     TKAGSDHLARAWGRTFGLPVLVSNCSNNYGPYQFPEKLIPHMILNALAGKPLPVYGDG
                     SQVRDWLYVEDHARALWTVLSEGAVGETYNIGGHNERRNLDVVHTICRTLKEAGEAPA
                     GGGRFEDLITFVTDRPGHDTRYAIDAGKIDRELGWAPAETFETGLASTVRWYLDNRGW
                     WERVLDGSYRLERIGVKAGEA"
     gene            5036..5914
                     /gene="rfbA"
                     /locus_tag="AC16QF_06980"
     CDS             5036..5914
                     /gene="rfbA"
                     /locus_tag="AC16QF_06980"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020797842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HIR6448335.1"
                     /translation="MNRRGIILAGGSGTRLHPITLSVCKQLLPVYDKPMIYYPLSTLM
                     LAGIREILIISTPEDTPRFEQMLGDGSQWGIELTYAVQPRPEGLAQAFIIGEDFVKGR
                     PSTLILGDNLFFGSGLSGLLRESAAVATGSSVFAYRVSNPESFGVVAFDENGIATSIE
                     EKPAKPKSRFAVTGLYFYDDQVVDIARSIKPSPRGELEITDVNNVYLRQGALNVQVMA
                     RGMAWLDTGTHDSLLEAAHFIQTLEKRQGLKVGCPEEIAWRAGWISDDALASLAAPLA
                     KSGYGDYLTGLLETGA"
     gene            5911..6453
                     /gene="rfbC"
                     /locus_tag="AC16QF_06985"
     CDS             5911..6453
                     /gene="rfbC"
                     /locus_tag="AC16QF_06985"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003173486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HIR6448336.1"
                     /translation="MKITQTRLPGVLVIEPNIYGDERGFFVETFQVGRYRDLAGIELP
                     FVQDNHSRSRYGVLRGMHAQRNHPQGKLVRVARGEVFDVALDIDPESPTFGQWAGATL
                     SDTNGQQMWIPPGYAHGFVVLSDVADFEYKCTDYYHADDEIGVLWNDPDAAIDWPIDD
                     PVLSDKDKALPTLAELRSAR"
     gene            6450..>7229
                     /gene="rfbD"
                     /locus_tag="AC16QF_06990"
     CDS             6450..>7229
                     /gene="rfbD"
                     /locus_tag="AC16QF_06990"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIR6448337.1"
                     /translation="MTVLVLGANGQVGTHLRDCLPDAVFWTRDKADLSRPTELEDAVL
                     AQKPSVIVNAAAYTAVDRAEEETDLAWTVNAEAPAALARAARELGAILVHFSTDYVFD
                     CTKAGPYLDTEPTRPQSAYGATKLAGELAVATLCPTHWIFRTSWVFSEHGGNFVKTML
                     RLGVERDELGVVDDQTGRPTYAGHIASAVAEVIGTGKAQALPWGIYHLTGGKPVSWCT
                     FAREIFRRAGDRGLMSSRPSVKAITTDEYPTPAKRPANSVLQ"
BASE COUNT         1262 a         2478 c         2313 g         1176 t
ORIGIN      
        1 tcccgcgctg tcggggtcac caggggcacg acccacggat aggcttcacg ctgcatgggc
       61 ccgatctcct gggcgttcag cgccagcggg ttggactcct cgccgcgcag cgcgaccacc
      121 tggtcgaagg ccggcgggtc ttccagatcc gtggagatgt tgtggatcgg cggcacggag
      181 taggccttgt cgaactgcat gcccatgacg atcagtatca caacgctgat cgcgaggccc
      241 aagtagatgg gcggtcggtt ctgtggaagc tcccgcttca ccgaggcgat gaggcccacg
      301 gcaccgacga tgacgccgat ggcggccagg atcgttccgc ccgcgagacc caggaagccc
      361 acctgaaaat cccacagccc gatgcgggtg cccagcgcgc ccgccaccag caggacggcg
      421 gcgatcaccg cgccgatgag tattgcccgc ggccaccagc tgttaacgtc tgcagccatg
      481 tcactcctcc ctgaattcgg ttacgcgcgg gagcatacct agacgatggt cgcgccgcca
      541 tcgatgacga aattctgccc cgtggtaaaa gcgccagccc tggacgctag gtacacggcg
      601 atgcccgcga tctcccgggg ctcgccgatc cgccgcagcg gatccccgga cgtgcgggct
      661 ttcaggatgt ccggattttc ccagagggcc cgggcgaagt ccgtgcgcac gagcccgggc
      721 gagatggtgt tcaccctgat gttcctcggc ccgtgctcgg ccgccaggtt cctcaccagc
      781 tgcgcgtccg ccgccttgct gatggcatag gcgccgaggt gggtgctgcc gcgcaatccg
      841 cccacggagg agacgacgat gatggatccg tcctcccgct ccagcatctc cggcaggacc
      901 atctggatca gccagtggtt ggacttgatg ttcacgtcca ggatcttgtc gaaggctgaa
      961 tccggcagct cggtcatgga gccgaagtac gggttgacgg ccgcgttgca gaccagcacg
     1021 tcgatcctgc ccagcttctc ccgcgtctgg gtcacgagcc tctccagctc ctccttatgg
     1081 ccgatgtggg ccggtatggc caccgcgccg ccggcctcac tggccagctc cgcgttgatg
     1141 gccgcagcca cctcgtcgca aaggtcggcc ttgcggctgg agatcaccac ctgggccccc
     1201 gcccgggcca tctcctcggc aatggcccgg ccgatgccct tgctggaacc cgtgatcagg
     1261 gccaccttgc ctgtcatatc gaatagatca ctcgccatgg gtcacatccc tgccttgacg
     1321 cccaccggag cgccgtccac cacctgatcg aggtactcag acaacccgta gccgacctgc
     1381 ccgtcgcagc ggtattccgt catgccctcc gtgatgcggg tcatgaggtc ctcgccatcg
     1441 ggcgtgcgcc gccggttgcg cagcgggatc agcgacatga ccttgccctc gacctcgtat
     1501 tcccgctcgt cggtgcgcgc ccagaccttg aaggtctcct ggcagtcgtt ggcgtcgtag
     1561 acggtctcga tgcgggcatc gcggatcatc acgtactcgt tgtcccgcag caccatgccg
     1621 cccgcccggg gctctgcgtc cggccggcag gtgatggaga ccatcatggc gaagtcctcg
     1681 ctgaacacca tgggcagcca gcggtaccag tagatggcct gccagaagcg cggcccccag
     1741 gagtggtccc gcaggcccag cccgtcgatc tcgaaacgct cgtcgcccac ccggatgaag
     1801 cccgcgcccc gggtgtgctg ctcggtgtgg ccccgggcga aggattcctc ggccttcatc
     1861 tcgatggcgc tgccgtcctc gttgacgggc tcgccgccga acatgggcga gatgccgtga
     1921 aagtcgagct cgatctcgct ttccaccacg gggttggtct tgaaggcgtt ggccggatcc
     1981 gccatctgct gcggatcggc cagcacgcaa agcttgccct ggtagcggac ggtcaggtgc
     2041 ttgaagggct ccaccacatt gaacgccatc ccgccgccgt ccatggcctc gttgtcgtgc
     2101 cgctccggcc gcttgaacat gaacccgatg cggccatcgg gcaggtagac gcagcaggac
     2161 atctcgccgc ggccttcgtt gggccggttg gccagccgga accagccgcc cgtgcggcgc
     2221 tcgcggtcga acacgttgaa gtacatgcgc tcgttgaaat tctgcgcttc ctcgacggcg
     2281 tgcaggtact cgtccgccgg ctcgaggcgc atgcggacgc ccggttgatc tgccatcgtc
     2341 acttccccct gttctgaatc ttcccgcccc ggctcggcgg ccgggacgag gtgccaatct
     2401 aaaggagggc gccccgccgc gtccaccgcg gggcacgccc gtcgcagcga cgatcggatg
     2461 cggattcggt atagtgcgcg gccttgaatc cacgtgcgac gcgaggacca aacgtgccgg
     2521 caaaatccat ggaggagctc gtctcgctgt gcaagcggcg cggcttcatc tttcagtcca
     2581 gcgacattta cggcggcctg cagggtcttt acgactacgg gccgctgggc gtcgagctga
     2641 agaacaacct gaaggccgcc tggtggcggg ccatggtgta cgagcgcgac gacgtggagg
     2701 ggctcgacgc ggctatcctg accaacccgc tgaccctgcg ctattccggc cacgaggaga
     2761 cctttaccga cccgctggtg gactgccgca actgcaagac gcgccaccgc gccgatcacc
     2821 tggagggcgg cacctgcccc gcctgcggtt ccagcgacct cacggacccg cggcccttca
     2881 acctgatgtt caagaccagc gtcgggcccg tggacgacgg ctcgagcttc gcctacctgc
     2941 gccccgagac cgcccaggcc atctttacga acttcaagca cgtcctggac tccacctccc
     3001 ggcgcctgcc cttcggcatc gcgcagatgg gcaaggcgtt ccgcaacgag atcacacccc
     3061 gcaacttcat ctttcgcgtg cgggagttcg agcagatgga gctcgagttc ttcgtcaagc
     3121 cgggcaccga cgaggaatgg catcacaagt gggtggaaga acgtctggac tggtgggcgg
     3181 cccagggcgt ggagcccaac agcctgcagc tctaccacgt acccgccgac gagctcgccc
     3241 actactcaaa ggcaaccgtg gacgtgatgt accggtttcc ccacggcctg gaggaactcg
     3301 agggtatcgc caaccgcacg gatttcgacc tcggctccca caccaaggcc cagggcgact
     3361 tcgacattca ggcccgggta ccccggaacc gggactccaa cgccaagctc gccatccagg
     3421 atctggagac gaacaagtgg gtggtgccct acgtgatcga gccctccgcc ggcgtggaac
     3481 gcggggtgct ggcggtgctg aacgaggcct atcgggtgga ggagctggac gacggcaaaa
     3541 cgcgtacggt gctgggcttc aagccccacc tggcccccat caaggcagcc gtgctgccgc
     3601 tgaagcgcaa ccacgaaggg atcgtgacga aggccaaggc catcaagcag cagctgcagg
     3661 cgctaggcct cggccgcatg ctctacgagg acacgggcaa catcggcaag ggctaccggc
     3721 gccatgacga ggtgggcacg cccatctgca tcaccgtgga tttccagacc atcgaggacg
     3781 acgacacggt gaccgtccgc gatcgggaca ccatggagca gcagcgcatc gcggtggccg
     3841 agctgcccgc ctacgtgacc gactacctgc gctgaagccg tgcgctgcgc cacaaaatcc
     3901 tggcaaaccg ggccgaaatc ctcgaccgga ggcgctatcc ttaggcattt cctcagcagg
     3961 gctttaacag cttgaagatt ctcgtcacag gcggcgcagg cttcatcggc tcggcggtgg
     4021 tgcgtcatct catcggcgcc acggacgccg aggtggtgaa tatcgacaag ctgacgtacg
     4081 cgggcaacct ggagtcgctg gcggacgccg cggacagtga gcgctacgcg tttgagcagg
     4141 ccgacatctg cgacgcggcg gcgcttgatc gaatcttcga ccagcaccgg ccggatgccg
     4201 tcatgcactt agcggcggag tctcacgtgg acagatccat cgacgggccc ggcgacttca
     4261 tccgcaccaa cctggtgggc acctacgagc tgcttcaggc ctcccggcgc tacctggacg
     4321 ccgaggcgcg cgcggcagac gacttccgtt ttcttcacgt ctccaccgac gaggtcttcg
     4381 gcgacctggc ggacacgggt gccttcttca cggaaaccac ctcttacgcg ccgagctccc
     4441 cctatgccgc caccaaggcg ggctcggacc atttagcgcg cgcctggggg cgaaccttcg
     4501 gcctgcccgt gctggtgagc aactgctcca acaactacgg gccctaccag tttccggaga
     4561 agctcattcc ccacatgatc ctgaacgcac tggcaggcaa gcccctgccc gtctacggcg
     4621 acggcagcca ggttcgggac tggctctacg tggaagatca cgcccgggcg ctgtggaccg
     4681 tgctgagcga aggcgccgtg ggcgagacct acaacatcgg cggccacaac gagcgccgca
     4741 acctcgacgt ggtccacacg atctgccgca cattaaagga agccggcgag gcgccggcgg
     4801 gtgggggacg gttcgaggat ctcatcacat tcgtcacgga tcggcccggg catgacaccc
     4861 gctacgccat cgacgccggc aagatcgacc gggagctggg ctgggcaccg gcggaaacct
     4921 tcgagacggg cctggccagc accgtacggt ggtatctgga caaccgcggc tggtgggaac
     4981 gcgtcctcga cggcagctac cggctcgagc gcatcggcgt caaggcgggc gaagcatgaa
     5041 ccggcgcggc atcattctgg ccggcggctc cggcacgcgg ttgcacccca tcaccctgag
     5101 cgtgtgcaag cagctgctgc ccgtctacga caagcccatg atctactatc cgctgtccac
     5161 gctcatgctg gcgggcatcc gggaaatcct gatcatctcg accccggagg acacgccgcg
     5221 tttcgagcag atgctgggcg acggcagcca gtggggcatc gagctgacct acgccgtcca
     5281 gccaagacca gagggcttgg ctcaagcctt catcatcggc gaggacttcg tgaaagggcg
     5341 cccgagcacc ctcattctcg gcgacaacct gttcttcggc agtggtttga gcggcctgct
     5401 gcgtgagtcg gcggcggtgg ccaccggcag cagcgtgttc gcctatcggg tcagcaaccc
     5461 ggaatccttc ggcgtggtgg cgttcgatga gaacggtatc gccacctcca tcgaggagaa
     5521 gccggcgaag cccaagagcc gcttcgcggt caccggcctg tacttctacg acgaccaggt
     5581 ggtggacatc gccaggtcca taaagccttc tccccgaggc gagctcgaga tcaccgacgt
     5641 gaacaacgtc taccttcgcc agggcgcgct caacgtgcaa gtcatggcga gagggatggc
     5701 ctggctcgac accgggaccc acgactcgct gctcgaggcc gcccacttca tccagaccct
     5761 ggagaagcgc cagggtctca aggtcggttg ccccgaggag atcgcctggc gggccggctg
     5821 gatcagcgac gatgcgctgg cgtccctggc cgcccccctc gccaagagcg gctacggcga
     5881 ctacctgacg ggtttgctgg agacgggggc atgaagatta cgcagaccag gctgcccggc
     5941 gtgctcgtca tcgagcccaa catctacgga gatgagcggg gcttcttcgt ggagacgttt
     6001 caggtgggcc gctaccgcga cctggccggc atcgagctgc ccttcgtgca ggacaaccac
     6061 tcccggtccc gctacggcgt gctgcgcggc atgcacgccc agcgcaacca tccccagggc
     6121 aagctggtgc gcgtggcccg gggcgaggtg ttcgacgtgg ctctcgacat cgatcccgaa
     6181 tccccgacgt tcggccagtg ggcgggcgcg accttatccg atacgaacgg ccagcagatg
     6241 tggatcccgc ccggctacgc tcatggcttt gtagtgctct cggacgtggc cgacttcgag
     6301 tacaagtgca cggactacta tcacgccgac gacgagatcg gcgtgctctg gaacgacccc
     6361 gatgcggcca tcgactggcc catcgacgac cccgttctct ccgataaaga caaggccctg
     6421 cccacgctgg ccgagctgcg gtcggccaga tgaccgtttt agtactcggc gccaatggcc
     6481 aggtgggcac gcacctgcgc gactgcctgc cggatgccgt cttctggaca agagacaaag
     6541 ccgatctctc ccggccgacc gagctggagg acgcggtgtt ggcgcagaag ccgtccgtca
     6601 tcgtcaacgc cgccgcctac accgccgtgg accgagccga agaggaaacg gacctcgcct
     6661 ggacggtcaa tgcggaggcg ccggccgcgt tggcccgcgc cgcccgggag ctgggcgcca
     6721 tccttgtgca cttctccacc gactacgtct tcgactgcac aaaagcgggg ccctacctgg
     6781 acacggagcc aacgaggccc cagagcgcgt acggcgccac caagctcgcc ggcgagctgg
     6841 ccgtggcgac gctgtgcccg acgcactgga tcttccgaac cagctgggtg ttcagcgagc
     6901 acggtggcaa cttcgtgaag accatgctgc ggctgggcgt cgagcgggac gagctcggcg
     6961 tggtggacga ccagaccggc cgccccacct acgccggcca catcgcctcg gccgtggccg
     7021 aggtcatcgg gaccggcaag gcgcaggcgc tgccctgggg catctatcac ctgacaggcg
     7081 gcaagccggt ctcctggtgc acgttcgcgc gggagatctt ccgccgcgcc ggcgaccgcg
     7141 ggttgatgtc tagccggccg tcagtgaagg cgatcaccac cgacgagtac ccgaccccgg
     7201 cgaagcggcc ggccaactcg gtcctgcag
//
LOCUS       DBNHLM010000150         2456 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1338473, whole genome shotgun sequence.
ACCESSION   DBNHLM010000150 DBNHLM010000000
VERSION     DBNHLM010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1338473"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="AC16QF_06995"
     CDS             <1..738
                     /locus_tag="AC16QF_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR6448338.1"
                     /translation="HVFGPSSLEAEDNILRLDRTLAELFAFVAEQVGLADTLIVLSAD
                     HGAPDSPGYLNSLGIPGGYVDPDSWDKADAIDRIKARFGIEGALFEKYAHPYLYFSPE
                     VAGNEQIDRGALEAAVIEELTKLPGVALAVSSTALGSASIPDSEIHRRVLRNHHPDRS
                     GDVFIVFEPNWFINDFDGLTVASTHGSPWRYDTHVPVVFAGARLRPAKIDRAVRTVDV
                     APTLSALLQIKAPSGSVGEPLVEVLRR"
     gene            740..1945
                     /locus_tag="AC16QF_07000"
     CDS             740..1945
                     /locus_tag="AC16QF_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR6448339.1"
                     /translation="MNARGLIVVASMLLVGSVGWAEPDEYAVDAEDDFELAAQEMADA
                     MADGGFELDDAKFAGNPMQRLFKKWPEDLVVAPIPSRSPQLGFQLSVVGGYFLGDRGE
                     DAKKRPSAIGGFAMASDNGSYAFGAGGKFHLLDDRLRLQAGAGYADIRYKFYGFGDFV
                     GDSGINIEIEQEAPLYFANAKWRFWNQLYAGIGFVRGNVETRTRVPLPPEWPREITPS
                     FNVDLGALWVPVQFDSRDHEQFPRRGWLIDGDTKIYRDDFGSDFEAETYKLALNHYRA
                     MRTRDALALRAVIKATGGNAPFFLLSSFGSGPDLRGYPAGRYRDKLMYAVQGEYRWHL
                     HDSWILTGFAGVGEVADEIGDFGDDFLPAAGIGARYVLSKKHRVSISADLAVGKHGAE
                     FYIGIGEAF"
     gene            complement(1956..>2456)
                     /locus_tag="AC16QF_07005"
     CDS             complement(1956..>2456)
                     /locus_tag="AC16QF_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448340.1"
                     /translation="ATVDDVLEALGPPHAMSAINDGYAFLYHWYAVEERQLGLSSNWP
                     VLRWFRLAIARADIDATALIVHFDDQGGVRAYGLDQRSIDLGGGTGFQPIFVVTPLVD
                     ADEYLEDIWPAHRWGMSMLREAPATLNAHAGVDSGMQGIEMRSTSLKVGQRTLEMQKE
                     QSSSSR"
BASE COUNT          413 a          786 c          808 g          449 t
ORIGIN      
        1 cacgtcttcg gaccgtccag cctggaggcg gaagacaaca ttctccggct cgaccgaacc
       61 ctggcggagc tgttcgcctt cgtcgccgag caggtgggcc tggccgacac gctgatagtg
      121 ctttccgccg atcacggcgc gccggattct cccggatacc tgaacagcct gggtatcccg
      181 ggtggctacg tcgacccgga cagctgggac aaggctgacg ccatcgatcg gatcaaagcg
      241 cgattcggga tcgagggcgc gctgttcgag aagtacgctc atccgtatct ctacttcagc
      301 ccggaagtcg ccggcaacga gcaaatcgac cgaggcgccc tggaagcggc ggtgatcgag
      361 gagctgacga agttgcccgg agttgccctg gccgtgtcca gcacggccct gggctcggcc
      421 agcattcccg actcggagat ccatcgccgc gttctgcgca accatcatcc ggaccgctcc
      481 ggcgatgtgt tcatcgtgtt cgagcccaac tggttcatca acgacttcga cgggctcacg
      541 gtggcctcca cccacggttc tccctggcgc tacgacaccc acgtgcccgt cgtgtttgcc
      601 ggcgctcggc tccgcccggc aaagatcgat cgtgccgtgc gcacggtaga tgtcgcgccg
      661 acgctgtccg cgctgctgca gatcaaggcg cccagcggct ccgttggcga gccgctggtc
      721 gaggtgttga ggcgatagga tgaatgcgcg cggactcatc gtggtggcct ccatgctgct
      781 tgttggctcg gttggttggg ccgaaccgga cgagtacgcc gtcgatgcgg aggacgattt
      841 cgagctggcc gcgcaggaaa tggcggatgc catggccgat gggggcttcg agctcgacga
      901 tgccaaattc gccggcaacc ccatgcagcg cctgttcaag aagtggccgg aggatctggt
      961 cgtcgcgccc atccccagcc gatcgcctca gctcggcttc cagctgtccg tggtcggggg
     1021 atattttctt ggcgacaggg gtgaagacgc aaaaaagcgc ccgtctgcca tcgggggctt
     1081 cgccatggcc tcggacaatg gcagctacgc gttcggggcc ggcgggaaat ttcacttgct
     1141 ggacgaccgc ctgcgcctgc aggcgggcgc gggctacgcc gatatccgtt acaagttcta
     1201 cggcttcggc gacttcgtcg gtgactcggg cataaacatc gagatcgagc aggaagcacc
     1261 gctttacttc gccaacgcca agtggcgttt ctggaaccag ctttacgcgg gaatcggctt
     1321 cgtccgcggc aacgtcgaga cgaggacgcg ggttcctctg cctcccgagt ggccacgaga
     1381 gatcaccccg tcgttcaacg tggacctcgg cgcgctgtgg gtgccggtgc agttcgacag
     1441 ccgcgaccac gagcagtttc cccgacgggg atggctgatc gatggggaca ccaagatcta
     1501 ccgcgacgac tttggcagcg atttcgaagc ggagacctac aagctcgctc tgaatcacta
     1561 tcgggccatg cgcacccggg acgctctggc cctgcgtgcg gtgatcaagg ccaccggtgg
     1621 taacgcgccg ttctttctgc tcagcagctt cggcagcggc cccgatctgc gcggttaccc
     1681 ggccggacgc taccgtgaca agctcatgta cgccgtgcag ggtgagtacc gctggcacct
     1741 tcacgacagc tggatcctga cgggctttgc cggggtgggt gaggtggctg acgagatcgg
     1801 cgacttcggc gacgacttcc tgccggcggc gggcattggt gcgcgctacg tgctgtcgaa
     1861 gaagcaccgg gtgagtattt ctgcggacct ggccgtcggc aagcacggcg ccgagttcta
     1921 catcggcatc ggcgaggcgt tctgaccgcg gccgttcagc ggctggagct gctttgctct
     1981 ttctgcatct ccagggtgcg ctgccccacc ttcagcgacg tcgagcgcat ttcaatcccc
     2041 tgcatgccgg aatccacgcc cgcatgggcg ttcagcgtgg cgggggcttc gcgcagcatg
     2101 gacatgcccc agcggtgggc gggccagatg tcctcgagat actcgtccgc gtccaccagg
     2161 ggcgtcacca cgaaaattgg ctggaagccc gtgcccccgc ccagatcgat ggatcgctga
     2221 tccaggccat aggcccggac gccaccctgg tcgtcgaagt gcacgatcaa cgcggtggcg
     2281 tcaatgtcgg ctcgggcgat ggccagccgg aaccagcgca acacgggcca gttggacgag
     2341 agcccgagct ggcgttcttc aaccgcgtac cagtggtaca ggaaggcgta accgtcgttg
     2401 atcgcgctca tggcgtgcgg tgggcccagc gcctccagca catcgtccac ggtggc
//
LOCUS       DBNHLM010000151         9232 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_500928, whole genome shotgun sequence.
ACCESSION   DBNHLM010000151 DBNHLM010000000
VERSION     DBNHLM010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9232
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_500928"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="AC16QF_07010"
     CDS             <1..212
                     /locus_tag="AC16QF_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012882.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448341.1"
                     /translation="SVRGTSVLADGRIEISGEAPLKDLQAYHSRLKSLTGGVGSFDMA
                     FSRYAQVTPQTAQELMGAFAGSDEE"
     gene            complement(265..1605)
                     /locus_tag="AC16QF_07015"
     CDS             complement(265..1605)
                     /locus_tag="AC16QF_07015"
                     /inference="COORDINATES: protein motif:HMM:NF016150.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HIR6448342.1"
                     /translation="MSEVTTSVTITTVISGPVRRSDRIDSLDVMRGVALLGILLMNII
                     AFGLPFATYYNPSVAGIDNPANLWAYIVNWLFFEGTMRALFSMLFGAGMVLMTTRAEE
                     RGAGIEAADVYYRRLLWLILFGVIDAYLLLWVGDILFLYGIAGLFLFVFRGLAPRTLF
                     LIGVAMLVVLGIKNVNASNELAELRQVTAEVESIRAAGGEPSREQLAAIDHLEEIRTE
                     FEPDPEQIQEQVEARHSGYIDQVKYLVPLNIAAQSTLTYTLGIWDVLMMMFMGMALIK
                     WGVFSAARSWGFYGAMVVVGYGIGLPINWYEVRMAIESGFAIEWSGIQYVSTYQIGRA
                     GTAAGHIGLIMMICKSGLFGFITKPLAAVGRMALTNYLAHSVICMFLFTGVGLAWFGE
                     LERYELYYVVGGIWLFQLIVSPIWLRYFRFGPMEWLWRSLTYAQTQPMRRSPPG"
     gene            complement(1698..2081)
                     /locus_tag="AC16QF_07020"
     CDS             complement(1698..2081)
                     /locus_tag="AC16QF_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HIR6448343.1"
                     /translation="MLELNPESVAQIAEKAREFQSEEILLPPEPPPVPVDDTAFEYAA
                     EWHGDPGYNDLKDAIEDLEPDQQSVLVALMWLGRGDYGIEEWNEALAYAEEVHTDATA
                     DYLIATPLLADYLLGGLEMHGYEPE"
     gene            2214..3353
                     /locus_tag="AC16QF_07025"
     CDS             2214..3353
                     /locus_tag="AC16QF_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR6448344.1"
                     /translation="MTENLRGLRLTSTVKSEGVLELALVESEVRSPGPDEVVVRIEAA
                     PINPSDLGLLFGLADMTTAEFSGSPERPVVQARIPGNFMKHMAGRLDEPMAVGNEGAG
                     VVVAAGASEAAQALMGRTVAVLGGAMYAQYRVLNVSMCLPLGEETSPREGAACFVNPL
                     TALGMVETMRMEGHSALVHTAAASNLGQMLQKICSADGVQLVNIVRKPEQVEILRGIG
                     AAHVVNSAAENFKDDLTEALATTGATLGFDAIGGGRLASDILACMEAAANRAATEYSR
                     YGSTTHKQVYIYGGLDLGPTQLHRSFGMYWGVGGWLLTPFLQKIGPEATQRLKERVAD
                     EVKTTFASHYAEEVSLAGALEPEAISAYGQKATGQKYLINPNAGL"
     gene            complement(3372..3818)
                     /locus_tag="AC16QF_07030"
     CDS             complement(3372..3818)
                     /locus_tag="AC16QF_07030"
                     /inference="COORDINATES: protein motif:HMM:NF025050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6448345.1"
                     /translation="MTMPDNTSITQMGYVVADLDAAMERWKTTVGAGPFERLNHVEVV
                     DGLYRGTPTNVDISVAIAEVGGIQLELIEQHNDVPSCYRDLFAAGKEGLHHVAVQPAD
                     FDAEVARYEALGCPQAFGGLYEGARFVYMDTSGSLGIMVELVERRE"
     gene            3875..5218
                     /locus_tag="AC16QF_07035"
     CDS             3875..5218
                     /locus_tag="AC16QF_07035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6448346.1"
                     /translation="MTAGSGTLHSDMAWPRQGYAWYVVIILFICSIFSFLDRQVIALM
                     VDDIKLDLGLNDFEIGLLQGPPFGIFYALMSIPIALAADRYSRRNIIIAGVAFWSAAT
                     AACGLAGNFWHLFLARVGVGSGEACLSPCAYSMISDYFRRQLLPLAMSIFTMGNLTGV
                     GLAMIIGGAVIGYAKSLDEIVIWGLGDVAPWQFAFIVIGLPGLLLAALIFTVREPLRR
                     GLSAPTAETDATSLRAFGGFVRQHWRTFATLFGSFTMLVLVAFGNFAWVPTFFIRTFG
                     WSEAYAGTIYGSIVAIFGTGGALFGGWFASYLMRRGYVDAPYRATLICSLPLAPLAVL
                     VFVVIPDGHWAAIVFAPYSFFSAVPAGHAATAMMTVTPNEMRAKISSAYLFFSNFIGL
                     SLGAATVGFLTHNVFGDDMMVGYSLTLVNGIGAPLAVIAIALGMKHYRRSVAELD"
     gene            complement(5215..6423)
                     /locus_tag="AC16QF_07040"
     CDS             complement(5215..6423)
                     /locus_tag="AC16QF_07040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6448347.1"
                     /translation="MRWLILAGLWLCYMSFGVVVTSLAPLVPVIQADLAMSHSAMGSI
                     LGAWQLVYIAAAVPCGILLDRLGGRWALTIGVGIIVVSAVSRAQADGYWALLAAVMLF
                     GIGGPIVSSGAPKIVTGIFTGSQRGLAMGIYMTGPTLGGIASLTLTHSLLLPMFDGDW
                     RAVMLLWSAVALAAAVAWFAIASICGPRVPGFDQGPIVSTAGRPSSRGVIAELSRAPA
                     VRLVLALAVGSFLINHGLNNWLPELLRSGGLGIVEAGYWAALPMVVGIIGSLTIPRLA
                     TPERRFRILFALCAAAAIASAALTFGGDVVLTFALVLQGFVRASLMTILILTLMELPV
                     VQERHAGTASGLFFSAAEIGGVLGPLGLGLMHDLTGGFNAGLYGLAAIAAAMALGAMR
                     LSGLAGARPG"
     gene            complement(6497..8851)
                     /gene="lon"
                     /locus_tag="AC16QF_07045"
     CDS             complement(6497..8851)
                     /gene="lon"
                     /locus_tag="AC16QF_07045"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR6448348.1"
                     /translation="MENSNNVKLPDNVLPIIPARNMVVFPGEVSHISIGRESSIAAAD
                     LAAQEGRKLGLLLQHDPAVDAPAADDLHHVGTIVSIVRYVKAPNGTRHLICQGEERFR
                     AIGFASGYPFLASYADTLSDVAEEGPEIAARTDLLKERITEITSLLPETPMEFATALA
                     AVEQPGALADLVAGFLSIDAETKQTILETRNLKDRLDAVLAAATERLEVLRISQEIGA
                     KSKEKIDKQQREYVLREQLKTIQRELGEEGAGPELAEVAEAIDAARMPEEADKEARKE
                     LKRLQSMPESSAEYSMVRTYLDWLTALPWSELDSEEIDIARARAILDDDHYGLEKIKR
                     RILEYLAVRKLNPDGRSPILCFVGPPGVGKTSLGQSIARALGLQFVRASLGGVHDEAE
                     IRGHRRTYIGALPGSIVQSMRKAGTRNPVFMLDEMDKLGAGFHGDPSSALLEVLDPEQ
                     NGTFGDNYLGVPFDLSKVLFIGTANVLENIPGPLRDRMEVIELPGYTQEEKLEIAERY
                     LIDRQLEATGLTKEQVSVDRAALVEVIDGYTREAGVRNLEREIGGLFRHAAMRITEDA
                     QAHVQIGAEDVAAILGPRKFEQEVQMRTSVTGVATGLAWTPVGGDILFIEATAVPGQG
                     KLILTGQLGDVMKESAQAALSLAKGRLASLPMEREWLDQNDLHVHVPAGAIPKDGPSA
                     GIAMFVAIMSLLSGRKVRSDIAMTGEVSLRGLVMPVGGVKEKTLAALRAGITTVALPA
                     RNRKDLEDIPASARKQLEFIWLETVEDAMTAAFELEPDDTAKAA"
BASE COUNT         1505 a         3006 c         3133 g         1588 t
ORIGIN      
        1 gctccgtgcg cggaaccagc gtcctggcgg acgggcggat cgagatttcc ggcgaggcgc
       61 cgctgaagga cctgcaggcc tatcattcca ggctcaagtc gctcacgggg ggcgtgggca
      121 gcttcgacat ggcgttcagc cgctatgccc aggtgacgcc gcagacggcc caggagctga
      181 tgggcgcgtt tgcgggcagc gacgaggagt agccggcggg caacgtcgag ggcgccgcgg
      241 cccgcagccc ggggagaggc cgccttaccc ggggggtgag cggcgcatgg gctgggtctg
      301 cgcgtaggtc agcgaccgcc agagccactc catggggccg aagcggaagt acctcagcca
      361 gatcgggctg acgatgagct ggaacagcca gatcccgccc accacgtagt agagctcgta
      421 gcgctcgagc tcgccgaacc atgccaggcc gaccccggtg aacagaaaca tgcagatcac
      481 gctgtgtgcc agatagttgg tcagcgccat gcgcccgacg gcggccaggg gcttcgtgat
      541 gaagccgaac aggccggact tgcagatcat catgatcaga ccgatgtgcc cggcggcagt
      601 ccccgcccgg cctatctgat aggtggagac gtactgaatc cccgaccact cgatggcgaa
      661 gcctgactcg atggccatgc ggacttcgta ccagttgatg ggcaggccga tcccgtagcc
      721 gaccaccacc atggccccgt aaaagcccca gctgcgcgcc gcggaaaata cgccccactt
      781 gatcagcgcc atgcccatga acatcatcat cagcacgtcc cagatgccga gcgtgtaggt
      841 cagcgtggac tgcgcggcga tgttcagggg aacgaggtat ttcacctggt cgatgtagcc
      901 ggagtgtctc gcctccacct gctcctggat ctgctcgggg tccggctcga actccgtccg
      961 gatctcctct aagtggtcaa tggcggccag ctgctcccgg cttggctcgc cgccggctgc
     1021 tcggatcgac tcgacctccg ccgtcacctg gcgaagctca gcgagctcgt tgctggcgtt
     1081 gacgttcttg atgccgagca cgacgagcat cgccacgccg atgaggaaca gggtgcgggg
     1141 cgcaaggccg cggaagacga agagaaagag gccggcgatg ccgtacagga agagaatgtc
     1201 gccgacccaa agaagcaggt aggcgtcgat gacgccgaag aggatcagcc acagcaggcg
     1261 ccgatagtac acgtcggccg cttcgatgcc ggcgccgcgc tcctcggccc gggtggtcat
     1321 cagcaccatc cccgcgccga acagcatgga gaacagcgcg cgcatcgtgc cttcgaagaa
     1381 cagccagttg acgatgtagg cccagaggtt ggcggggttg tcgatcccgg caacgctggg
     1441 gttgtagtag gtggcgaacg gcaatccgaa cgcgatgatg ttcatgagca ggatgcccag
     1501 aagcgccacg ccgcgcatga cgtccagcga gtcgatcctg tcgctgcgcc gaaccgggcc
     1561 ggaaatcacg gtcgttatcg tgacggaggt ggtgacctcg ctcatgcccc tctcctgaaa
     1621 cctcccgcag cctggagttt cggcggattg aacggctttg gcaaccgggt gaacccggcc
     1681 gtcgtgggag cgccgcgcta ttcgggctcg tagccgtgca tctccaggcc tcccagcagg
     1741 tagtcggcca gaagaggggt tgcgatcagg tagtcggcgg tcgcgtcggt gtgcacctcc
     1801 tcggcgtagg cgagcgcttc gttccactcc tcgatgccgt agtcgccccg gccgagccac
     1861 atcagcgcca ccagcaccga ctgctggtcg ggttcgagat cctcgatggc gtccttcaga
     1921 tcgttgtagc ccggatcgcc gtgccactcg gccgcatact cgaaagccgt gtcgtccacg
     1981 ggaacggggg gcggctccgg gggcagcagg atctcctcgg attgaaactc ccgcgctttc
     2041 tcagcgatct gcgccacgct ctcgggattg agttcgagca taccgcctgc ctcctcgttt
     2101 cattctgtca ccaaccagcg cttattcttg ccgttctacg gagcaagcaa atcaggggtt
     2161 gtacataggc cataacggtg tacggtctcc gggcgaagaa aggaggacgc atcatgacgg
     2221 agaatctgcg aggcctgagg cttacctcga cggtaaagag tgagggcgtg ctcgagctgg
     2281 ccctggttga atcagaggtg cgaagccccg gccctgacga ggtggtggta cgcatagagg
     2341 cagcgcccat caacccgtcc gaccttggcc tgctcttcgg cctggcggac atgaccacgg
     2401 cggaattctc gggctcgccg gagcgtccgg tggtgcaggc acggatcccc gggaatttca
     2461 tgaagcacat ggccggtcgg ctggacgagc ccatggcggt tggcaatgag ggtgcgggcg
     2521 tcgtggtggc ggcgggcgcc tccgaggctg ctcaagcgct catgggccgg accgtggccg
     2581 tgctgggggg cgccatgtat gcccagtatc gggtgctgaa cgtgtccatg tgcctgcccc
     2641 ttggcgagga aacttccccg cgtgaggggg ccgcctgctt cgtgaacccg ctgacggctc
     2701 tggggatggt ggagaccatg cgcatggagg gtcactcagc tctcgttcat acggccgcgg
     2761 cctccaacct cggccagatg ctgcagaaga tctgctcagc cgacggcgtg caactggtca
     2821 acatcgtccg caagcccgag caggtggaga tcctgcgggg gattggtgcc gctcacgtgg
     2881 tgaactccgc ggccgagaac ttcaaggacg atctcaccga ggcgctggcg accacggggg
     2941 cgaccctggg attcgatgcc atcggcgggg gccggctggc cagcgacata ctcgcctgca
     3001 tggaagccgc cgcgaaccgg gcggccactg agtacagtcg ctacggatcg acgacccaca
     3061 agcaggtcta catctacggc ggtctcgatc tcggccccac gcagctgcat cgctccttcg
     3121 ggatgtactg gggcgtgggg ggctggctcc tcacgccgtt cctgcagaag atcggtcccg
     3181 aggcgacgca gcggctgaaa gagcgggtgg cggatgaggt gaagaccacg ttcgcgagcc
     3241 actatgccga ggaggtctcg ctggccggcg ccctggaacc cgaagcgatc tccgcctacg
     3301 ggcagaaagc cacgggccag aaatatctga tcaatcccaa cgccgggctc tgaaccaccc
     3361 cggctggcgc gtcactcccg tctctccacg agctcgacca tgatgcccag cgagccggac
     3421 gtgtccatgt agacgaagcg ggcgccttcg tagaggccgc cgaaggcctg ggggcagccg
     3481 agcgcctcgt agcgcgccac ttccgcgtcg aaatccgccg gctgcacggc cacgtggtgc
     3541 aggccctcct tgcccgcggc aaagagatcc cggtagcagg agggcacgtc gttgtgctgc
     3601 tcgatgagct cgagctggat gccgcccacc tcggcgatgg ccacggagat gtccacgttc
     3661 gtgggcgttc cccggtacag gccgtcgacg acctcgacgt gattcaggcg ctcgaacggg
     3721 cctgcgccga cggtggtttt ccagcgctcc atggcagcgt cgagatcggc gaccacgtag
     3781 cccatctggg taatgctggt gttatctggc atagtcatcg gctgtcccct gcgcagcgag
     3841 agttaaggat aaccttgtcc ggacccccat caccatgacc gccggctccg gaacgctgca
     3901 ctccgacatg gcctggccgc ggcagggcta cgcctggtac gtggtcatca tcctgttcat
     3961 ctgctcgata ttctccttcc tcgatcggca ggtcatcgcg ctgatggtgg acgacatcaa
     4021 gctggacctg ggcttaaacg atttcgagat cgggttgctg caggggccgc cgttcggcat
     4081 tttctatgcg ctgatgtcca ttcccattgc gctggccgcc gaccgctaca gccggcgtaa
     4141 catcatcatc gccggcgtgg cgttctggag cgcggccacc gccgcctgcg gccttgccgg
     4201 caacttctgg catctgtttc tcgcccgggt cggcgtgggc agcggagaag cgtgcctgtc
     4261 accgtgcgcg tactccatga tcagcgacta tttccggcgt cagcttctgc ccctggccat
     4321 gagcatcttc accatgggca acctcaccgg cgtcgggctc gccatgatca tcggcggggc
     4381 ggtgatcggc tatgccaaga gcctggacga gatcgtgatc tggggtctcg gcgacgtggc
     4441 accctggcag tttgccttca tcgtcatcgg cctgccgggg ctgctgctcg ctgcgctcat
     4501 cttcaccgtc cgcgagccgc tgcgccgcgg cctgtcggcg ccgacggcgg agacggacgc
     4561 cacttcgctc agagccttcg gagggttcgt tcgccagcac tggcgcacgt tcgccaccct
     4621 gttcggcagc ttcaccatgc tggtgctggt ggccttcggc aacttcgcct gggtccccac
     4681 gttcttcatc cgcaccttcg gctggtccga agcctacgcc gggacgatct acggctccat
     4741 cgtggccatc ttcgggacgg gcggcgcgct gttcggcggc tggtttgcca gctacctgat
     4801 gcggcgcggc tacgttgacg cgccttaccg ggcgaccctc atctgctcgc tgcctctggc
     4861 gccgctggcg gtgctggtat tcgtggtcat tcccgacggg cactgggcgg ccatcgtctt
     4921 cgctccttat tcgttcttca gcgcggtccc cgcgggccac gcggccacgg ccatgatgac
     4981 cgtgacgccc aacgagatgc gggccaagat ctcgtcggcc tacctgttct tcagcaactt
     5041 catcgggctt tcgcttggtg ctgcgacggt gggctttctc acccacaacg tgttcggtga
     5101 cgacatgatg gtgggctact ccctgacgct cgttaacggc atcggcgcgc cgctggcggt
     5161 gatcgccatc gcgctcggaa tgaagcacta ccggcgcagc gtcgcggagc tggactagcc
     5221 gggcctggct ccggcgagcc cggagagcct catcgcgccc agggccatgg cggccgcgat
     5281 ggcggccagc ccatacagcc ccgcgttgaa cccgccggtg aggtcgtgca tcaggccgag
     5341 ccccagcggg ccgagcacgc cgccgatctc ggctgccgag aagaacagcc cgctcgccgt
     5401 ccccgcatgg cgctcctgca ccaccggcag ctccatcagc gtcaggatca ggatggtcat
     5461 cagcgaggcg cgcacgaacc cctgcagcac cagggcgaag gtcagcacca cgtcgccgcc
     5521 gaaggtgagt gccgcgcttg cgatggcggc cgccgcgcac agggcgaaca gaatgcggaa
     5581 gcggcgctcg ggggtagcca gccggggtat ggtcagggag ccgatgatgc ctaccaccat
     5641 gggaagcgcc gcccagtagc cggcctccac gatgcccagg cccccgctgc gcagcagctc
     5701 gggcagccag ttgttgagac cgtggttgat gaggaacgaa cccaccgcca gtgcaagcac
     5761 cagccgcacg gccggcgcgc gggagagctc cgcgatgaca ccgcggctgc tgggccgacc
     5821 ggccgtggag acgatggggc cctgatcgaa gccaggcacg cggggaccgc aaatggatgc
     5881 gatggcgaac cacgcaacgg cggccgccag cgccacggcg ctccacagca gcatcacggc
     5941 gcgccagtcg ccgtcgaaca tggggagcag cagggagtgg gtcagggtca gggaggcgat
     6001 gccgcccaga gtagggccgg tcatgtagat gcccatggcc aggccgcgct ggctgccggt
     6061 gaagataccc gtgacgatct tcggggcgcc ggaggagacg atggggccgc cgatgccgaa
     6121 cagcatcacc gctgccagca gcgcccagta gccgtctgcc tgggcccggc tgacggcgga
     6181 gacgacgatg atccccaccc cgatggtcag cgcccagcgc ccgcccaggc ggtcgagcag
     6241 aatgccgcag ggaacggcgg cggcgatgta gaccagctgc caggcgccga ggatgcttcc
     6301 catggccgag tgggacatcg cgaggtcggc ctggatgacg gggaccaggg gagccaggga
     6361 ggtcaccacc accccgaagg acatgtagca cagccacagg ccggcgagga tcagccagcg
     6421 cacgggcgtg caactccgga gcgcacgtgg tgcgggtgat cgcaggcgcg ggcgcccgcg
     6481 atacccatcc cggcgctcag gcggccttcg cggtgtcgtc gggctccagc tcgaaggccg
     6541 ccgtcatggc gtcctccacc gtctccagcc agatgaactc cagctgcttg cgggctgacg
     6601 ccgggatgtc ctccaggtct ttgcggttgc gggccggcag ggccacggtg gtgatccccg
     6661 cgcgaagcgc ggcgagcgtc ttctccttga cgccgcccac gggcatgact agcccgcgca
     6721 ggctgacctc gcccgtcatg gcgatgtcgg aacgcacctt gcggccggag agcagcgaca
     6781 tgatggccac gaacatggcg atcccggcgc tcggcccgtc tttgggtatg gcgccggcag
     6841 gcacgtgcac gtgcagatcg ttctggtcga gccattcccg ctccatgggc agtgaggcca
     6901 ggcgcccttt cgccaggctc agcgcggcct gggcgctttc cttcatgacg tcgcccagct
     6961 ggccggtcag tataagcttg ccctggccgg ggaccgccgt cgcctcgatg aagagaatgt
     7021 ctccgcccac cggtgtccag gcaagccccg tggcgacgcc cgtcacgctg gtgcgcatct
     7081 ggacctcctg ctcgaatttg cgcggcccca ggatggcggc cacatcctcg gcaccgatct
     7141 gcacgtgagc ctgcgcatct tccgttatgc gcatggcggc atggcggaac aggccgccga
     7201 tctcgcgctc taagttcctt accccggctt ctctcgtata gccgtcgatg acctcgacga
     7261 gggcggcgcg atccaccgag acctgctctt tggtcaggcc ggtcgcctcg agctgcctgt
     7321 cgatcagata gcgctcggcg atctcgagtt tctcctcctg ggtgtagccg ggcagctcga
     7381 tgacctccat gcgatcccgc aacgggccag ggatgttctc cagcacgttg gcggtgccga
     7441 tgaacagcac cttggagagg tcgaagggca cgccgaggta gttgtcgccg aacgtgccgt
     7501 tctgctctgg gtccagcacc tcgagcagcg ccgaggacgg atccccgtga aacccggcgc
     7561 cgagcttgtc catctcatcg agcatgaaca cggggttgcg ggtgcccgcc ttgcgcatgc
     7621 tctggacgat ggagccgggc agggcgccga tgtaggtgcg ccggtgcccg cggatctcgg
     7681 cctcgtcgtg cacgccgccg aggctggccc gcacgaactg caggccgagc gcccgggcga
     7741 tgctctgacc gagggacgtc ttgcccacgc cgggcggacc cacgaagcag aggatggggc
     7801 tgcggccgtc cgggttcagc ttgcgcacgg cgaggtactc gaggatgcgg cgcttgatct
     7861 tctccaggcc gtagtggtcg tcgtccagaa tggcccgtgc ccgggcgatg tcgatctcct
     7921 ccgagtcgag ctctgaccac ggcagcgcgg tcagccagtc caggtacgtg cgcaccatgg
     7981 agtactcggc cgaggactcc ggcatgctct gcaggcgctt gagctccttg cgcgcttcct
     8041 tgtcagcctc ctccggcatc cgggctgcat cgatggcttc cgccacctcg gcgagctctg
     8101 gcccggcacc ttcctcgccg agctcccgct gaatggtctt cagctgctcg cgcaggacgt
     8161 actcgcgctg ctgcttgtcg atcttctcct tgctcttggc gccgatttcc tgggagatgc
     8221 gcagcacctc caggcgctcg gtcgccgcgg cgaggacggc gtcgagccgg tctttgaggt
     8281 tcctcgtctc caggatcgtc tgcttggttt ccgcatcgat gctgagaaaa ccggcgacca
     8341 ggtcggccaa tgcgccgggc tgctccacgg cggccagcgc cgtggcgaac tccatggggg
     8401 tctccggtag cagcgaggtg atttccgtga tcctttcctt gagcagatcg gtccgcgcgg
     8461 cgatctcggg gccttcttcc gcgacgtcgg acagcgtgtc ggcataggag gccagaaacg
     8521 ggtagccgct ggcgaagccg atggcgcgga agcgctcctc gccctggcag atgaggtgcc
     8581 gggttccgtt cggggccttc acgtagcgca cgatgctgac gatagtgccg acgtggtgga
     8641 gatcgtctgc cgcgggcgcg tccacggcgg gatcgtgctg cagcagcagg ccgagcttcc
     8701 ggccctcctg ggcggccaga tcggcggccg ctatcgatga ctcccggccg atggagatgt
     8761 gggagacctc ccccgggaac accaccatgt tccgggcggg tatgatgggc agcacattgt
     8821 ctggcagctt gacgttgttc gagttttcca tgctttgggg tggcgcatcg tgcgcggatg
     8881 gtggagatgc ttgggatatg tgggcgcgga gggggcgttt caagtaggtc cgaccggccc
     8941 ccatcgagcc cgtcgcggcg gcccggtgcc ccgggccgtg gcgcgcccat tggggcgggg
     9001 ttttccgcta gacagtcaaa ggtgactgtt ttagagtccg cagtgaggtc ctgcaggaga
     9061 gagcaggaaa ttttgggagt gcgccccggc cttcggggaa atcagcacag caaggggagt
     9121 tctatgaaga agctagttag aggcggtgga gcgtgcagct atgcggtcgt tttggcctgc
     9181 gcggcgtttc tgccggctgg cgaggccgtc gcagaggaga cggccagagc gt
//
LOCUS       DBNHLM010000152        10685 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_501134, whole genome shotgun sequence.
ACCESSION   DBNHLM010000152 DBNHLM010000000
VERSION     DBNHLM010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10685
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_501134"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="AC16QF_07050"
     CDS             <1..878
                     /locus_tag="AC16QF_07050"
                     /inference="COORDINATES: protein motif:HMM:NF040138.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR6448349.1"
                     /translation="PSGAPQAYSEHVVDTSRPTCRSWPRGPWIYSSDTFGATEGGSSG
                     SPVVNEAGQVVGQLSGACGYETSNVCSDLNATVDGAFAAYFNDVAQWLDPAPTSSGNN
                     PPTASFRWSGDPDVMFTDTSTDLDENDSISSWAWNFGDGLTSTATSPSHTYACNGTYN
                     VTLTVEDTNGASDSASNAVTITAADVCENLTIGVKDPLAGSIDESGRRPAVTFRINVA
                     TSDDGGDPNGAVVSGDWGGLVKGSGSCTVNGSSCEVIKGNIRGSGDVTFTVTGIELSG
                     YTFDGTEVTGTVQVP"
     gene            947..1396
                     /locus_tag="AC16QF_07055"
     CDS             947..1396
                     /locus_tag="AC16QF_07055"
                     /inference="COORDINATES: protein motif:HMM:NF025559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HIR6448350.1"
                     /translation="MSETRLTNLERTRIEMAYAVPLIRDLQRLLGAEVINDALSRRFD
                     DQIDATAAPGEKADFSRMEAGTATFAEGGALDYEIIGSDAEHFDLNITGCRYAEMMDE
                     LGARDIGHLLICNRDYPAAAKIGMALTRTQTRMQGAGYCDFRYRRRG"
     gene            complement(1397..1741)
                     /locus_tag="AC16QF_07060"
     CDS             complement(1397..1741)
                     /locus_tag="AC16QF_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HIR6448351.1"
                     /translation="MINIEHDYFSDEATPLAEIEAAGYHAVPLDFPAEENENHWHDFN
                     SLLYITEGELTVTYAETGETCTCGPGTKITGVPNVVHREKTEGYKALIGIPVPPAELT
                     QPINKPLPAPAG"
     gene            1905..2996
                     /locus_tag="AC16QF_07065"
     CDS             1905..2996
                     /locus_tag="AC16QF_07065"
                     /inference="COORDINATES: protein motif:HMM:NF014954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6448352.1"
                     /translation="MTNDELAVPETPADVTPAWLTRALGQRWPGSEVRSVVGEPLGEG
                     IGQISLLQRLTLEHAGAAEAPRHMVLKMHTTHADMLGVAVRFRMYEREANFYNRFADR
                     VPLRTPEVYHAATEGLERCVMLMEDMSSWHSPDQLAGPTLEEAELAVDSLATLTAAFW
                     NSPELSDHASWLPDFNVGYMSDTGNDYRQCLPEFLRRYETKLPPGSADAARTIADRYD
                     DLTRILSTGPQVLTHYDYRLENMFFRNDAAEAFCALDWQLVMRARPGWDIAYFMGTNL
                     PVSFLDEHGEALRDRYYQQLQSNGVQGYARADLEADLAIGTMAMTGIPIIGGANADLS
                     NPRNEALFTEVGSRAFGAVIGNDCLAYLP"
     gene            complement(3022..4080)
                     /locus_tag="AC16QF_07070"
     CDS             complement(3022..4080)
                     /locus_tag="AC16QF_07070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6448353.1"
                     /translation="MADDYRERMQAYAQRLMGYKTGEMVSLMVHIGDELGLYRAMAGQ
                     DSVTAESLAEATGLHERWVAEWLRGQGAAEILDHVGDERFRLSDVAEQALLNEDSPAY
                     LQGFFSRPPSHEIVDRTIEAFSTGVGVSWGAHGDSASHFLCRTNRPFHMKLADEVIPL
                     LGGMSERLEAGGAVLDVGCGPGTALTELARAFPNSQFVGVDPAANMIAQAQQRYADMD
                     NVRFEVGYGETLEDVAAYDLVTTFDCMHDMTDPEGTMRAVRRAVKGDGAWLIKDIKAQ
                     DSYAENLENPVAAMMYGFSVLFCMSSALSKPGGAGLGTLGFPPSVCERMGHDAGFGQF
                     RILDFEGDPFNFYYELRP"
     gene            complement(4149..6896)
                     /locus_tag="AC16QF_07075"
     CDS             complement(4149..6896)
                     /locus_tag="AC16QF_07075"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR6448354.1"
                     /translation="MRSVFRFMCFMLFVSAGPASLARGAVDVDQLAIPHETFVLENGL
                     TVIVHEDHSVPIVAVNLWYHVGSRNESRGRTGFAHLFEHFFFNGSEHYPHDFRRAMDD
                     VGANNRNGTTNPDRTNFFEDVPVSALERTLYLEADRMGFLAANITQETLDQERGIVQN
                     EKRQGENQPYGKVWNRIVESIYPYSHPYSWPIIGSMADLEAATLDDVQAWYAKYYGPS
                     NCVLSLAGDITVEQARELVGKYFAGIPPGPPVERYRQWVPTLERPLRDVMHDRVPQAR
                     IYRVYHTPGWLDAESVLIELNAGLLSGSRSAPLDRALVYDKQVATEVAAFAWDKEIAG
                     NLVVVVSVKPGVDPADAERELDAVMAQFLGQKPARSALDRARTRQVAQFIRGMERLGG
                     FGGRSDILAESMTYAGDPEAFLQRYRKVMEATPAELHAASTKWLDANHYTLTVLPFPE
                     LKAAAEDVDRTSLPALGPAPEVAFPEIGSRELDNGLRVLLLERRGAPLVNMSLAVDAG
                     YAADPAAAAGTASLALQLLEEGTRSRDGFALADDLDALGATISSASSLDLSFVQLRAL
                     KANLARSLAVFADVVRNPAFAGDMVALGKRRRLAQIAQEEAQPFSAALRMVPPLLYGD
                     GHVYAKPLTGSGYVETVRNIEPDDLSKWHSAWFRPNNATLIVTGDVTFDDLMPMVDDV
                     LGDWKPGAVPLKRVADVPRGKAGRIYLVDRPDASQSVIVAAQASIPGGQAEEVAIEAA
                     MRAFGGMSTSRFNQNLRIDKHWSYGVFGALLDARGHRPLLVVAPVQTDRTADSMAEIV
                     GEFRSILGDRPIAGEEFASLKRNMELRLPGRFETLASLEDAAHDLVNYGYSPAFYYDY
                     AKTVRALGEQDLAEAVNRVINPEQLIWVVVGDLEKIEAQVRALGFGEVVRLKASGASG
                     D"
     gene            complement(7127..7648)
                     /locus_tag="AC16QF_07080"
     CDS             complement(7127..7648)
                     /locus_tag="AC16QF_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448355.1"
                     /translation="MKGKVLFFSLVFYAVVGMWLWGEYQQRPRVLDGLGQVMLGMTPA
                     QVVGVLGRPTEEFAPAEDEQRRLEYSYRSDGTSDYLVVFWPDEEGEPKADIICREGGS
                     VFGLAVGQSSELDIIDKLGEPSHRSFGPDGRAKAISYESFKVAFEIEGGWTREICVSQ
                     TGSISYALESITV"
     gene            7880..9706
                     /locus_tag="AC16QF_07085"
     CDS             7880..9706
                     /locus_tag="AC16QF_07085"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HIR6448356.1"
                     /translation="MADIFVSYASEDLDRVKPLITALEERRFAVWWDREIHPGTSWDE
                     AIERELESATWIVAIWTANSIRSRWVRTESMAALERDALIPILLDDVTLPVAFRTTQV
                     VDLRQWRPEGSDATFERLVSHLQGKPHPPAEVEAHRQRSPPNRRGPLLAALALTALAA
                     IALVWMVVTEPVRAPSDAGLAVTTFAGQPRIAVLPFDHGSDPEDRPFANGLTDEVIKG
                     LQQYRTIPVISRHASFALRDKGLSVQEIASQLGARYLVTGAVSRRGDRLRVSAGLTDA
                     DGHQLWKESYEVDFDPESVFALQDQIAEQIAGVSYPQILEGEIDRVARRDVTSLDAWG
                     HAVNAVEIINAMNVDRLDEGLLHAERALELDPNLPLAYWARAEIKVYQLVERGLTGQS
                     ADAMEDEILEDFQEALDISPFDGALCGCLGFVRVMRGEIEQAEAVFDTVLPVNPSNAI
                     LRTNYATLLTHQGKLEEARREAELAIRLDPLSRFGSMSWSTLSLVAAMDNDLEQAIQL
                     ARRALQVEGTETYGQVQLPMLLYLKDRIPEAQDAFADLLKHHPRVNPRNRLTYGWFRP
                     IEPLIREHVAQASGTLRKERSLADLLEWIFLDLGWEPPTPMP"
     gene            9886..>10685
                     /locus_tag="AC16QF_07090"
     CDS             9886..>10685
                     /locus_tag="AC16QF_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448357.1"
                     /translation="MQIRTQAAAVLIVLLAGGCAVQQPVQPTGIANITRDQIGRMAVR
                     SPDRPKVALAANVDTKGAAARKTALSAGATWLGGSFEAAGQAGEGGLLIAAFGLVTAP
                     IVAAGGAVYGAAAADSKEEITSGNEVLVRTLDFAPAQMQRALENELTTSAPMDWEFVD
                     PDASNAELAARGFDSVLDMDMDSIASHVSANDFHVYFDTLNRLTLTSLVSGQSLAIRK
                     YWRELPPRAVSDWAGDGGSVLTNALHEQFADIAREMADDLFLYPAIRV"
BASE COUNT         2013 a         3537 c         3341 g         1794 t
ORIGIN      
        1 acccgtccgg cgcgcctcag gcctactcgg agcacgtggt cgatacaagc cggcctacct
       61 gccgcagctg gcccagaggc ccttggatct acagcagcga caccttcggc gccaccgagg
      121 gcggcagcag cggctctccg gtcgtaaacg aggcaggcca agtcgtcggc caattgtccg
      181 gcgcttgcgg ctacgaaacc tccaacgtct gcagcgacct caacgctacc gtggacggtg
      241 cgttcgccgc gtacttcaac gacgtcgctc aatggctcga tccggcaccg acgagcagtg
      301 gcaacaaccc gcccacggcg agcttcagat ggtccggcga tccggacgtc atgttcaccg
      361 acacaagcac cgacctcgat gaaaacgaca gcatctccag ctgggcctgg aatttcggcg
      421 acggcctaac cagcacggca acgagcccga gccacaccta cgcctgtaac gggacgtaca
      481 acgtcacgct cacggttgaa gataccaacg gcgcaagcga ctccgcgtcg aatgccgtca
      541 cgatcactgc cgccgacgtc tgcgaaaatc tgacgatagg cgtaaaagac cccctggcgg
      601 gcagcattga tgaaagcggc cgccggccgg cggtgacctt taggatcaat gtggccacca
      661 gcgatgatgg cggcgacccg aacggcgctg tggtatccgg tgattggggc ggactggtca
      721 aagggtccgg ctcctgcacc gtgaacggtt cgtcctgcga ggtgatcaag ggcaacatcc
      781 gcggcagcgg tgacgtcacc tttaccgtca cgggcatcga gctctccgga tacaccttcg
      841 acggcacaga agtcaccggc acggttcagg tcccctgagc ctcgggtagt catggagcag
      901 gcgcggccgc aaaccgcgcc ttccgcacag aatgggggaa ccacccatga gcgagaccag
      961 actcaccaac ctggaacgta cgcgcatcga gatggcctac gcggtgccgc tgatccgcga
     1021 tctgcagcgg ctgctcggcg cggaagtgat caacgatgcg ctttcacgcc ggttcgacga
     1081 tcagatcgat gcgacggccg caccgggcga gaaggccgac ttctcccgga tggaggccgg
     1141 taccgcgacg ttcgccgagg gcggcgcgct ggattatgag atcatcggct ccgacgccga
     1201 gcatttcgac ctgaacatca ccggctgccg atacgcggag atgatggacg agctcggtgc
     1261 ccgggacatc ggccacctgc tcatctgcaa ccgcgactac cccgccgccg cgaagatcgg
     1321 catggccctt acccgcaccc agacgcgcat gcagggcgcc ggctactgcg acttccgcta
     1381 ccggcgccgg ggctgatcag ccggccggtg cgggcagcgg cttgttgatg ggctgggtaa
     1441 gctctgccgg cggcacggga atgccgatca gcgctttgta gccttccgtc ttctcccggt
     1501 gcacgacgtt cgggacaccg gtgatcttgg ttccgggccc gcacgtgcag gtctcgccgg
     1561 tctcggcgta agtgaccgtc agctcgccct cggtgatgta cagcaacgag ttgaaatcgt
     1621 gccagtggtt ctcgttctcc tcggcgggga agtccagggg aacggcgtgg tagccggccg
     1681 cctcgatctc cgccaggggc gtcgcctcgt cggaaaagta gtcgtgctcg atgttgatca
     1741 tgttgctccc ctcatccgga tcgaacgtac acccacattc cggcctctgt ccactcttgc
     1801 atgatcgcac cggtcgatca ggtgtcacgc gccctgtccg ggttgtccca ccgtttactc
     1861 cgatctccgg ccgatttagg ctccgaggca gggaggatac acgcatgacc aacgatgaat
     1921 tagcggtacc tgaaacaccc gcagacgtca cgcccgcctg gctcaccagg gcgctcggcc
     1981 agcgctggcc ggggagcgag gtgcgcagcg tggtcggcga acccctcgga gagggcatcg
     2041 gtcagatcag cctgctgcaa cgcctgaccc tcgagcacgc cggagctgcg gaggcgcccc
     2101 ggcacatggt gctcaagatg cataccaccc acgccgacat gctgggggtt gcggtgcgtt
     2161 tccggatgta cgagcgggag gcgaatttct acaaccgatt tgcagaccgg gttccgcttc
     2221 ggactccgga ggtctaccac gcggctacgg aagggctcga gcgctgcgtc atgctgatgg
     2281 aggacatgag cagctggcac tcacccgacc agctggctgg ccccactctg gaagaggccg
     2341 agctggccgt ggacagcctg gcgaccctga cggcagcctt ctggaactcg cccgagctgt
     2401 cggaccatgc cagctggctg ccggatttca acgtgggtta catgagcgac acgggcaacg
     2461 actaccgcca gtgcctaccc gagtttctgc gccgctacga aacgaagctg ccccccggct
     2521 ccgccgacgc agcacgcacc atagccgacc ggtatgacga cctgacacga atcctcagca
     2581 cgggccccca ggtgctgacg cactacgact accgtctgga aaacatgttc ttccgcaacg
     2641 acgcggcaga ggccttctgc gccctggact ggcagctggt gatgcgggca cggcctgggt
     2701 gggacatcgc ctacttcatg ggcacgaacc tgcccgtgtc ttttctggac gagcacggcg
     2761 aggctttacg cgaccgctac taccaacagc tacagagcaa cggggtccag gggtatgcgc
     2821 gcgccgattt agaggccgac ctcgcaatcg gcaccatggc catgacgggc atacccatca
     2881 tcggcggcgc caacgcggac ctgtccaacc cgcgcaacga agccctcttc accgaggtcg
     2941 gcagccgggc tttcggcgcg gtgatcggca acgactgcct ggcctacctg ccttgattca
     3001 ggaaccgatc aggcaacgcc gtcagggtct gagctcatag tagaaattga acggatcgcc
     3061 ttcgaagtcc aggatccgga actgaccgaa gcccgcatcg tggcccatgc gctcgcagac
     3121 gctgggcggg aatcccaggg tgccgagccc ggcgccgccc ggcttcgaga gcgccgatga
     3181 catgcaaaac agcactgaga aaccgtacat catcgcggcc accgggttct ccagattctc
     3241 ggcgtagctg tcctgtgcct tgatgtcctt gatcagccag gcgccatctc ccttaacagc
     3301 tctgcgcacc gcccgcatgg ttccctccgg atccgtcatg tcgtgcatgc agtcgaaggt
     3361 ggtgacgagg tcgtaggccg ccacgtcctc cagcgtctcg ccgtagccca cctcgaagcg
     3421 cacgttgtcc atgtcggcat agcgctgctg agcctgagcg atcatgttgg ccgctggatc
     3481 gacacctacg aactgcgagt tgggaaaggc tcttgccagc tcggtcagcg ccgtgccagg
     3541 accgcaaccc acgtccagga cggcaccacc ggcttcgagt cgttccgaca ttccgcccag
     3601 cagggggatc acctcgtctg cgagcttcat gtggaaaggc cgattggtcc ggcagagaaa
     3661 atgggaggcc gaatcgccgt gggcgcccca gctgaccccc acacccgtgc tgaaagcctc
     3721 gatggtccgg tcgacgatct cgtgggacgg cggccgggag aagaagccct gcagatacgc
     3781 ggggctgtcc tcgttcagaa gcgcctgctc ggccacgtcc gacagcctga accgctcgtc
     3841 gccaacgtga tcgagaatct ccgcggcgcc ctggccgcgc agccattcgg caacccaccg
     3901 ctcgtgcagg ccggtggcct cggccaggga ttcagccgtc acgctgtcct gtcccgccat
     3961 ggcccggtaa aggccgagct catcgccaat gtgcaccatc agagacacca tctcgccggt
     4021 cttgtagccc atcagacgct gggcgtaggc ctgcatccgt tcccgataat cgtccgccat
     4081 ggcaagtttc cccgttaccg ttgtatcccc gcggcgtttc gccgccgcat cccgcagcaa
     4141 gccagcgctc agtcgccgga agcacccgac gccttgagcc tgaccacctc gccgaaccct
     4201 agtgcgcgca cctgcgcctc tatcttctcc aggtcgccga ctaccaccca gatgagctgc
     4261 tcagggttaa tcacccggtt caccgcctcg gccagatcct gctcgccgag cgcccgcacg
     4321 gtcttggcgt agtcgtagta gaaggccggc gagtacccgt agttgaccag atcgtgcgcc
     4381 gcgtcttcca gtgacgccag cgtttcgaaa cgcccgggca acctcagctc catgtttcgt
     4441 ttgaggctcg caaactcctc tccggcaatg ggccgatctc ccagaatcga acgaaactca
     4501 ccgacgatct cggccatgga atccgcggtc cggtcggtct gcaccggcgc gaccacgagc
     4561 agcggacggt gcccgcgcgc atcgagcagc gcaccgaaca cgccgtagct ccagtgcttg
     4621 tcgattctga ggttctgatt gaaccgggag gtcgacatgc cgccgaaggc ccgcatcgcc
     4681 gcctcgatag ccacttcctc cgcctgcccg ccgggaatgg acgcctgagc ggccacgatg
     4741 accgattgcg acgcgtccgg ccgatcgacg agatagatgc gacctgcttt gccacggggt
     4801 acgtccgcga cgcgcttgag cggaacggcg ccgggcttcc agtctcctaa cacgtcatcc
     4861 accatgggca tgaggtcgtc aaaggtcacg tcaccagtga cgatcagcgt cgcgttgttg
     4921 ggcctgaacc aggcggagtg ccatttagag agatcgtccg gctcgatgtt gcgcaccgtt
     4981 tcgacatatc cggatcccgt aagcggcttg gcgtagacgt ggccatctcc gtacagaagc
     5041 ggcggcacca ttcgcagagc tgcggagaac ggctgcgctt cctcctgagc gatctgcgca
     5101 agccggcgac gcttacccag cgcgaccatg tccccggcaa atgccggatt gcgaaccacg
     5161 tcggcaaaca cggcgagcga acgggcgaga ttcgctttga gcgcgcgcaa ctgcacgaag
     5221 gatagatcaa gggagctggc cgagctgatg gtggctccaa gcgcgtcgag atcgtcggcc
     5281 agcgcaaagc catcacggga tcgggtgccc tcttccagca gctgaagcgc aagcgaagcc
     5341 gtgcccgccg cggcggccgg gtcggccgcg taccccgcgt ccacggccag cgacatgttc
     5401 acgagaggcg cgccgcgccg ctcgaggagc agcacccgca aaccgttgtc gagctcgcgg
     5461 gaaccgatct cgggaaaggc gacctccgga gccggcccca gcgctggcag cgaggtgcga
     5521 tccacgtctt cagcggcagc tttgagctcc gggaacggca gcacggtgag ggtgtagtga
     5581 ttcgcatcga gccatttcgt cgaagccgcg tgcaactccg ccggcgttgc ctccatcact
     5641 ttccggtagc gctgcaggaa ggcttccgga tcgcccgcgt aggtcatgct ttcggccaga
     5701 atgtccgacc ggccgccaaa gccgccgaga cgctccatgc cgcggatgaa ctgcgcaacc
     5761 tgtcgggtcc tggcgcgatc gagagcactt cgcgcaggct tctgaccgag gaattgggcc
     5821 atcacggcat ctagctcccg ctccgcatcg gccgggtcca cgccgggctt gacggagacc
     5881 accaccacga ggttgcccgc gatctccttg tcccaggcga aggcggcgac ctccgtggcc
     5941 acctgtttgt cgtagacgag ggccctatcc agcggcgcgc tgcggctgcc gctgagcagc
     6001 cctgcgttca gctcgatcaa cacggattcg gcgtcgagcc agcccggcgt gtgatagacg
     6061 cgatagatgc gtgcctgagg aacccggtcg tgcatcacgt cgcgcagcgg ccgctccagc
     6121 gtgggaaccc actgacgata acgctccacc ggaggacccg gaggaatccc tgcgaagtac
     6181 ttgccaacga gttccctggc ctgctcgacg gtgatgtcgc ccgccaggga cagcacgcag
     6241 ttggacggac cgtagtactt ggcgtaccag gcttggacat cgtcgagcgt ggccgcttcg
     6301 aggtcggcca tgctgccgat gatgggccag ctgtaggggt gcgaataggg gtagatcgac
     6361 tccacaatgc ggttccagac ctttccatag ggctggtttt cgccctgacg cttttcgttc
     6421 tgaacgatgc cgcgctcctg atccagagtc tcctgagtga tgttggcggc gagaaagccc
     6481 atgcggtcgg cttccagata gagcgtgcgc tcgagcgcgg agacaggcac gtcctcgaag
     6541 aagttcgtgc ggtccgggtt ggtcgtgccg ttgcggttgt tggcgccgac gtcgtccatg
     6601 gcgcgacgga aatcgtgcgg gtagtgctcg ctgccgttga agaagaagtg ctcgaagagg
     6661 tgcgcaaagc ccgtccggcc ccggctttcg ttacgggatc ccacgtggta ccagaggttc
     6721 acggcgacga tgggcacgct gtggtcctcg tgcacgatca cggtgagacc gttttcgagc
     6781 acgaacgtct cgtggggtat ggccagctgg tcaacgtcaa cggcgccccg ggcaaggctt
     6841 gccggtcctg ctgagacaaa caacataaaa cacatgaacc ggaagacact gcgcatcagt
     6901 cacacctcgt tgtcgccatg gctcgcgaga acacggcgat caccggccat ccggcctgcg
     6961 ccacttccgc cgggctgaag cgcgagtatc gcatccggat cactggaagc aaccgcccgg
     7021 gggaaacggc tgatcgaaaa gggaagtgaa attcgccagc agcgtgtcca ggtcgatgct
     7081 gccgctgtgc gactccctgg cgtccagcca gtgcccgctg ctgaatctag acggtgatcg
     7141 attccagcgc gtagctgatg gagcccgttt ggctgacgca gatctcccgc gtccatccgc
     7201 cctcgatctc gaaggcaacc ttgaagctct cgtaagagat ggcctttgcg cggccgtccg
     7261 ggccgaagct gcgatgggac ggctcaccga gcttgtcgat gatgtcgagc tcggaagact
     7321 gacccacggc cagcccgaag acgctgccgc cttcccgaca gatgatgtca gccttcggct
     7381 cgccctcttc gtccggccag aaaaccacga ggtaatcgga cgtaccatca ctgcggtagg
     7441 agtactcgag gcgccgctgt tcgtcctccg cgggggcaaa ctcctcggtc ggccggccca
     7501 gaacacccac cacctgcgcc ggcgtcatgc caagcatcac ctgccccaac ccgtcgagga
     7561 cgcgaggtct ttgctggtac tcgccccaca gccacatacc gacgacggcg tagaacacca
     7621 gcgagaagaa caggaccttt cctttcattg cgggattcgt ttccttgagc agccaatccg
     7681 tggcacaggc caccgcgcct gaagattctg cacgttagat cgagaaaacg ccttgcgctg
     7741 aggcgcggga agtttgtgac ccagcgcaca atcccagtgg acgcgggagc gaaaaggccg
     7801 tccgggaaga cgagtgcaca ttctgctagg cttccattca cttgatcatg ccggccggac
     7861 ctgggcggaa gcgggatgca tggcggacat cttcgtctcc tacgcaagtg aggacctcga
     7921 tcgcgtaaaa ccgctgataa ccgcgttgga ggaacgcaga ttcgccgtgt ggtgggaccg
     7981 cgagatccac cccggcacca gctgggacga ggcgatcgaa cgcgagctcg agtcggcaac
     8041 gtggatcgtg gcgatctgga ccgccaactc catacggtcc cggtgggtgc gcacggagtc
     8101 gatggccgct ctcgagcgcg acgcgctcat accgattctt ctcgacgacg ttacccttcc
     8161 cgtggctttc cgtacgactc aggtagtgga cctgcggcaa tggcggccgg aaggatcaga
     8221 cgcgacgttc gaaaggctgg taagtcacct ccaggggaaa ccgcacccgc ccgccgaagt
     8281 cgaggcgcac cgacaacgat cgcctccaaa ccggcgcggc ccgctcctcg cagcactggc
     8341 cctaaccgcc ctcgccgcca ttgccctggt ctggatggtc gtgaccgaac cggttcgagc
     8401 accgtccgac gcgggcctcg ccgtgaccac gtttgccggg caaccaagga tagccgtgtt
     8461 gccgttcgac cacggcagcg accccgagga tcgcccgttc gccaacggac tgaccgacga
     8521 ggtcatcaaa ggtctgcagc aatatcgcac catcccggtg atatcgcggc atgccagctt
     8581 tgcgttgcgg gacaaggggc tgtccgtcca ggagatcgcg agccagcttg gcgcgcgcta
     8641 cctcgtgacc ggagccgtca gccgccgggg cgacaggctc agagtcagcg ctggcctgac
     8701 cgatgctgac gggcaccagc tctggaagga gtcctacgag gtggacttcg accccgagtc
     8761 ggttttcgcg ctccaggacc agatcgccga gcagatcgcc ggcgtgagct acccgcagat
     8821 tctcgaggga gaaatagacc gggtagcgag gcgcgacgtc accagcctgg atgcctgggg
     8881 gcacgccgtg aacgcggtag agatcataaa cgccatgaac gtcgatcggc tcgacgaagg
     8941 actactccat gccgagcggg ctctcgagct ggatccgaac cttcctctcg cgtactgggc
     9001 aagggcggaa ataaaggtgt atcagctcgt cgagcgcggc ctcaccgggc agtcggcgga
     9061 cgccatggag gacgagatcc tcgaggactt ccaggaagcc ctggacatca gcccgttcga
     9121 cggcgcgctg tgcggctgcc tggggttcgt gcgcgtcatg cgcggcgaga tcgaacaggc
     9181 cgaggcggtg ttcgataccg ttctgcccgt caacccgagc aacgccatcc tgcgcacgaa
     9241 ctacgcgacc ctgctgactc atcagggcaa gctggaagag gcccggcgcg aggccgagct
     9301 ggccatccga ctggaccccc tcagccgttt cggttccatg tcctggtcta ccctgagcct
     9361 ggtagcggcc atggacaacg acctcgagca ggcgatccag ctggcccggc gggcgcttca
     9421 ggtggagggc accgaaacat acggtcaggt gcagctgccg atgctgctct acctgaaaga
     9481 ccgcattcct gaggctcagg atgccttcgc cgatctgttg aagcatcacc ccagggtgaa
     9541 tccccgcaac cggctcacct atggttggtt ccggcccatc gagcccctga tccgcgagca
     9601 cgtggcccag gcgtccggca cgctgcgaaa ggagcggtcg ttggccgacc tgctggaatg
     9661 gatctttctg gatctgggct gggaaccgcc aaccccgatg ccctgacggc tcgacgtggc
     9721 gcttcgacgt ccacgtgttt acgggcgcaa ggccaaattt aaatataaat aaatcaaata
     9781 gttaccggcg cctaagaaaa ccacaacaag cttcagccgc acatcaggat ctgcgcgcgc
     9841 ggcgatgcga ggctgcctgc cacgtacacg cagggagaca gccagatgca gatccgaacc
     9901 caagccgccg ccgtcctcat cgtgcttctg gccggaggct gcgcggtcca gcaacccgtt
     9961 cagcccacgg ggatcgcgaa catcacccgg gaccagatcg gccgcatggc cgtgcgcagc
    10021 ccggatcgtc ccaaggtggc gctggcggcg aacgtggaca cgaagggcgc ggcagcacgc
    10081 aaaacggcgc tctcggcggg ggcaacctgg ctgggcggat ccttcgaagc ggcaggtcaa
    10141 gccggtgagg gaggattgct gatcgccgcc ttcgggctcg tcaccgcccc catcgtcgcc
    10201 gccggagggg ccgtctacgg ggccgcagcc gctgattcca aggaagagat cacttccgga
    10261 aacgaggtgc tcgttcggac gctggacttc gcgcccgcgc agatgcagcg ggcactggag
    10321 aacgagttga cgacgtcggc gcccatggat tgggaattcg tcgaccccga tgccagcaac
    10381 gcggagctcg ccgcgcgcgg cttcgactcc gtgctggaca tggacatgga cagcatcgcc
    10441 agccacgtga gcgcaaacga cttccacgtc tatttcgaca ccctgaatcg tctgaccctg
    10501 accagcctcg taagcggcca gtcgctcgcc atcagaaagt actggcggga gcttccgccc
    10561 cgggcggttt ccgattgggc cggtgacggc ggcagcgtcc tgaccaatgc cctgcacgag
    10621 cagtttgccg acatcgccag agagatggcg gacgacctct ttctctaccc cgccatccgg
    10681 gtcca
//
LOCUS       DBNHLM010000153         1763 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1458807, whole genome shotgun sequence.
ACCESSION   DBNHLM010000153 DBNHLM010000000
VERSION     DBNHLM010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1458807"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..976
                     /locus_tag="AC16QF_07095"
     CDS             299..976
                     /locus_tag="AC16QF_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448358.1"
                     /translation="MKWLLAELVVVVGGVFVALLLEGVFEERQDRRDEQLVLRALHEE
                     FVANQTELERQYSNYERRLAAAEVFLDLGSSAADLPADSVETLWRWYLRGGTFDPAMG
                     TLDGLLATGRLDLIQDAHLRASLAAWPSRLENMRHLELTITDFIGQQVYPWLREQTAL
                     PPGGFGESGVPEGRFPYDPEVVHSSRTFENLTRELVGWDRAMLLDRAEVDQVIGDVLM
                     ALSLQMR"
     gene            1250..1531
                     /locus_tag="AC16QF_07100"
     CDS             1250..1531
                     /locus_tag="AC16QF_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR6448359.1"
                     /translation="MIKTFADRDTERLFERQRVKRFGPAVQRAGLRKLRMLDAAEALE
                     DLRIPPANRLEKLKGDRAGQHSIRINRQWRICFIWRDGDAFDVEIVDYH"
     gene            1543..>1763
                     /locus_tag="AC16QF_07105"
     CDS             1543..>1763
                     /locus_tag="AC16QF_07105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HIR6448360.1"
                     /translation="MTTTLLAPVHPGEVLLEEFLEPMGISQYRLAKDISVPPRRINEI
                     VHGKRSVTADTALRLSRYFGTSERFWLNL"
BASE COUNT          348 a          526 c          546 g          343 t
ORIGIN      
        1 ccgcctccac gtaccacggc tcttccagcc ccaaaatcgt cctgtacagc gtccgatcgt
       61 ccatcgcctg actccccttt caagaagcca gtcaccctac tccgcccact cccttcccgg
      121 aagagccaca aggaattgct gctgacgcgg tgagcagttg acgctcgcgc ctccggcgct
      181 caaagggcat cttggtcgcg cggcagaatt ccgatccgtt agagcgcatc gctcagacgg
      241 gctgctattc atcatcccat atcgaaagcc gagccttttg agacctccgc aacagaccgt
      301 gaagtggcta cttgccgagc tcgtcgtagt tgttggcggt gtcttcgtcg cacttctact
      361 tgagggtgtg tttgaggagc gtcaggaccg tcgtgacgag caactcgtcc tccgggccct
      421 ccatgaagag ttcgtcgcga atcagacgga gctggaacgt caatactcga actacgagcg
      481 acgcttggct gcggctgagg tgttcttgga ccttgggtcg agcgcagcag accttccggc
      541 cgactctgtc gagacactat ggcgctggta tctccggggc gggacattcg accccgccat
      601 gggcaccctg gatgggttgc tggcgacggg tcggctagat ctgatacaag acgcccacct
      661 tcgggctagc cttgctgcat ggcccagccg acttgagaac atgaggcatc tcgagctcac
      721 gatcaccgac tttatcggcc agcaggtcta tccgtggctc cgggaacaga cggctctacc
      781 gccgggaggc tttggcgagt caggggttcc agaaggccga ttcccatatg accccgaagt
      841 ggtgcactct agtagaacct tcgaaaacct gacgagggag ttggttggct gggaccgggc
      901 catgctcctc gatcgggcgg aagtggacca ggtcattgga gacgtactca tggctctgtc
      961 tttgcagatg cgctgagctg caaaggcggg tggtcggatg cgctctaaca aggaattgct
     1021 gctgtccgcg gacgctggtc ggtaggtcgg gtggcacctg cggtttgtca gcgcacgggt
     1081 gcggcagcag aattccgagc cgttagagcg caccgcaggc taccagggtc tcgatggagg
     1141 gcgtgaggcc gaggaggaag atgatgggcc aaacgattcg cgagacacca gcgctcccgg
     1201 aacagtagta acgctggacg gtaataacgt aacgcgttac tgtgcgtgag tgatcaagac
     1261 tttcgctgac cgggacaccg aacgcctttt cgagcgccag cgggtgaagc gcttcggccc
     1321 tgccgtccag cgcgcgggac tccgcaaact acggatgctc gacgcggccg aggcgttgga
     1381 ggacctgcgt atcccgccag cgaaccgatt ggagaaattg aaaggggatc gagccgggca
     1441 acacagcatc cgcatcaacc gtcagtggcg gatctgtttc atctggcgtg acggcgatgc
     1501 gttcgacgtc gagatcgtcg actaccactg agggtcatgg caatgacgac cacacttttg
     1561 gcgccggtcc accctggcga agtcctacta gaggaattcc tcgagccgat gggcattagc
     1621 cagtaccggc tcgccaagga catcagcgtc ccaccgcggc gcatcaacga aatcgttcac
     1681 ggcaagcggt cggtgaccgc cgatacggcg ttgcgcctct cgcgatactt cgggacctcg
     1741 gagcggttct ggctgaacct cca
//
LOCUS       DBNHLM010000154         5013 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_739814, whole genome shotgun sequence.
ACCESSION   DBNHLM010000154 DBNHLM010000000
VERSION     DBNHLM010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_739814"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1703
                     /locus_tag="AC16QF_07110"
     CDS             <1..1703
                     /locus_tag="AC16QF_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HIR6448361.1"
                     /translation="NPLNEVFHFSVEREDGIGVEVAMQWNDSFQEQVFAYTNNIPQGD
                     GGTHLAGFRGALTRSLNNYIEREGLLKKIQVSTTGDDAREGLTAVVSVKVPDPKFSSQ
                     TKEKLVSSEVKPAVEQELARYFTEYLDENPQDARAVVSKMIDAARAREAARKAREMTR
                     RKGALDIAGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRRTQAILPLRGKIL
                     NVEKARFDKMLSSVEVGTLVTALGCGIGRGEFDPEKLRYHRVIIMTDADVDGSHIRTL
                     LLTFFFRQMPELIERGHIYIAQPPLYKVKKGRQERYVKDDDELNGYFLQLALEGAKLH
                     VNVGAPALDDGALESLAQDYRRLLDRLAAWSRVYPVELTERLVELAPVDEAMLADEEA
                     MLSWSKDLISALGLEDADPVVRRDPEHQTYCPTVTLVENGMSSDYSLSSGYFSSQEYR
                     MTVAMGERLNGLLEPEAYVQRGEKQQAVATFGEALAWLLREARRGLDIQRYKGLGEMN
                     PAQLWETTMDPEVRRMLKVTVDDAVAADQMFTTLMGDQVEPRREFIEANALSVVNLDV
                     "
     gene            complement(1755..2474)
                     /locus_tag="AC16QF_07115"
     CDS             complement(1755..2474)
                     /locus_tag="AC16QF_07115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6448362.1"
                     /translation="MAGLRSLVFFAGYAVITIAWGALSLLIAWLMPYRARFLFIVGAW
                     TRMILAWLRLTCGIRCKVEGRENIPEQPCIVLARHESTWETLFLQSLFSPQATLIKRE
                     LLWIPFFGWAFALLRPIAIDRSEPRKALRRLINQGRERLESGIWVVMFPEGTRMAPGK
                     PGKFQAGGAALAAATEAPVVVVAHNAGSFWPAHEFRKRPGTITLRIAPAIETSGKSTK
                     QINQEAAKVMADLAPAPTRQS"
     gene            complement(2474..3013)
                     /gene="gmhB"
                     /locus_tag="AC16QF_07120"
     CDS             complement(2474..3013)
                     /gene="gmhB"
                     /locus_tag="AC16QF_07120"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016852304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HIR6448363.1"
                     /translation="MARLILLDRDGVINFDSPDYIKSPDEWRAIPGSIEAIAALRSGG
                     RIVAVCTNQRGIARGKFSLSDLEAIHAKMSKALADAGTRVDAVTFCPHAADAGCNCRK
                     PKPGMLLSMMERFQVSPEETVFVGDTIRDLEAAAAAGCAAVLVRSGKGPEEEAEALRR
                     GLSFTAYDDLAQFTRAMGP"
     gene            3065..3649
                     /locus_tag="AC16QF_07125"
     CDS             3065..3649
                     /locus_tag="AC16QF_07125"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007160143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR6448364.1"
                     /translation="MVKARRCGWCGDDPLYVAYHDREWGMPERDPTVLFERLVLEGMQ
                     AGLSWLTILNKRENMRERFFAFDPARLAAAGEAEVAAWLNDPGIIRHRGKLEAMISNA
                     RAYLRQGDFSNLLWSAVGGAPRQNRWRHLGDVPSQTGESRDLARMLKSAGFGFVGPTT
                     CYAFMQSAGLVNDHLVSCPAHQRCADAWTALCGG"
     gene            3637..4521
                     /locus_tag="AC16QF_07130"
     CDS             3637..4521
                     /locus_tag="AC16QF_07130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6448365.1"
                     /translation="MRRLKGWLILALLRATSILPLAAARALARAAAAAALTLDVRAAR
                     TTRINVALCFPELGPEEQARLVRDSLAHSASVVAEAGPLYHWPRARWQTRLTLVGDEL
                     FNEAMASGRGVLLLGPHFGNWEALPLFLGRYAVTGLYDPPRISALEKTVRAARERNGM
                     RLLPIDARGLRGVYQALSAGSAAGILPDQVPGRNAGVYAPFFGHPALTMTLAHRLIRR
                     TRPVVLLVAARRVSGGFRIEFHAAPPDILAPDPETSAAAMNSAIESLVRTDPSQYQWE
                     YKRFRRQPAGVADPYRVG"
     gene            complement(4531..>5013)
                     /locus_tag="AC16QF_07135"
     CDS             complement(4531..>5013)
                     /locus_tag="AC16QF_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014443.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HIR6448366.1"
                     /translation="DFSVWPSAAPVLLMFAAFAGGCAGSTAGGVKMIRVLLIYRQGIR
                     EIRRLIHPQGVFPIKLGRHRVPDRVVDAVWGFFSFYVIIFLAMVACLMLISDLDFLTA
                     FSAVGACLNNLGPGLGEVALNYAALPVSVKWLLIFAMLLGRLEIFTLLVLMTPAFWRR
                     "
BASE COUNT          930 a         1550 c         1647 g          886 t
ORIGIN      
        1 agaacccgct caatgaggtc tttcacttct cggtcgaacg ggaagacggc atcggcgtag
       61 aagtagccat gcagtggaac gacagctttc aggagcaggt gttcgcgtac accaacaaca
      121 tcccgcaggg tgatggcggc acccatctgg ccggattccg cggcgcgctt acccgatcgt
      181 tgaacaatta catcgagcgg gaggggctgc tgaaaaagat tcaggtctcc accacggggg
      241 acgacgcccg tgaaggtctg acggctgtag tgtcggtgaa ggtgccggat ccaaagtttt
      301 cttcacagac caaggagaag ctggtttcca gcgaggtcaa accggccgtg gagcaggagc
      361 ttgcccgata ctttacggag tatctcgacg agaaccccca ggatgccagg gccgtggtgt
      421 cgaagatgat cgacgcggcg cgagcccggg aagccgcgcg caaggccaga gaaatgactc
      481 gccgcaaggg cgccctggac atcgccgggc tgccgggcaa gctggcggat tgtcaagaaa
      541 aggaccccgc gctgtcagag ctttatctgg tggagggcga ctccgccggc ggctccgcca
      601 aacaggggcg cgatcggcgc actcaggcca tccttcccct gcgtggaaaa attctcaatg
      661 tggagaaggc gcgttttgac aagatgcttt cctctgtgga ggtcggcacg ctggtcactg
      721 cccttggatg tggaatcgga cgtggggaat tcgatccgga gaagcttagg taccatcggg
      781 tcatcatcat gaccgacgcg gatgtggacg gatcgcacat ccgcacgctg ctcctcactt
      841 tcttcttccg gcagatgccc gagttgatcg agcgtggcca catctacatt gcgcaaccgc
      901 ctttatataa ggtgaagaag gggcggcagg agcgatacgt caaggacgat gacgaattga
      961 acggctattt tctgcagctt gctctggagg gcgccaagtt acatgtcaat gtaggcgccc
     1021 ccgccctcga tgacggtgcc ctggagtcgc ttgctcagga ttatcggcgg ctgttggacc
     1081 gactggccgc ctggtcccgc gtctatccgg tcgaattgac cgaacgattg gtcgagctgg
     1141 ctccggtcga tgaggccatg cttgccgacg aagaggcgat gctcagctgg agtaaggact
     1201 taatttccgc gctaggcctg gaagatgcag atccagtcgt gcgaagggac cctgagcacc
     1261 agacctattg ccccaccgtt acgctggtgg aaaacggcat gtcgtcggat tattccctga
     1321 gctcaggcta cttctccagc caggagtacc ggatgaccgt tgccatggga gagcggttga
     1381 acggcttgct ggaacccgaa gcctacgtgc agcgcggcga aaagcagcag gcggttgcca
     1441 cgttcggcga ggccctcgcc tggctgctgc gggaagcgcg ccggggtttg gacatacaac
     1501 gatacaaggg cctgggggaa atgaaccccg ctcagctgtg ggaaaccacc atggatcccg
     1561 aagtgcggcg catgctcaag gtaaccgtgg atgatgcggt ggcggcggac cagatgttca
     1621 ccaccctcat gggagatcag gtggagccgc gacgggagtt catcgaggcc aacgcgctgt
     1681 ccgtggtgaa cctcgacgtc tgagcgcttt gtttggggca aggcgcgaaa gccggcccct
     1741 tcgcccccac acggtcagct ttgtcgtgtt ggcgcgggcg ccaggtccgc catcacctta
     1801 gccgcttctt gattgatctg cttggtggat ttcccgctcg tctcaatggc tggtgcgatc
     1861 cggagggtga tcgtaccggg gcgctttcga aattcgtggg caggccagaa gcttcccgca
     1921 ttgtgtgcga ccaccacgac gggtgcttcc gtggcggcgg cgagggcagc gcccccggcc
     1981 tgaaatttcc cgggctttcc gggcgccatt ctggtgccct cgggaaacat gacgacccaa
     2041 attccgcttt ccagccgctc gcgcccctgg ttgatgaggc ggcgcagggc ctttcggggc
     2101 tcgctgcgat cgatggcgat gggccgcagc agcgcgaatg cccagccgaa gaaaggaatc
     2161 caaagcagct cccttttgat cagggtggcc tgaggcgaga acaggctctg caggaacagg
     2221 gtttcccacg tgctttcgtg acgggccagc acgatgcagg gttgctccgg tatgttctcc
     2281 cggccttcaa ccttgcagcg gatgccgcag gtcagcctga gccaggccag tatcatgcgc
     2341 gtccacgccc cgacgatgaa cagaaatcgg gcgcgatagg gcatgagcca ggcgatcagg
     2401 agcgaaagcg ccccccacgc gatcgtgatg accgcgtaac cggcgaagaa cacgagagat
     2461 ctcagtcccg ccatcatggc cccatggctc gcgtaaattg ggcaagatcg tcgtacgcgg
     2521 tgaagctgag cccgcgccga agggcttcgg cttcctcctc cggccctttt ccggaccgga
     2581 ccagaaccgc cgcgcacccg gcggcagcgg cagcctccag gtcgcggatg gtgtcgccca
     2641 cgaacaccgt ctcttccggg gatacctgga atcgctccat catggagagc agcatacccg
     2701 gctttggctt gcggcaattg cacccggcgt ctgcggcatg ggggcagaat gtcaccgcat
     2761 ccacgcgggt tccagcgtcg gcgagcgctt tgctcatctt cgcgtggatg gcttccagat
     2821 cgctcagcga gaacttgccg cgagcgatcc ctcgctggtt ggtgcacacg gccacgatgc
     2881 ggccgccgct tctcagcgca gcgatggctt cgatggatcc ggggatagcc cgccattcgt
     2941 cgggtgactt gatgtagtcg ggcgaatcga agttgatcac gccgtcgcgg tccagcagga
     3001 tgagtctggc catcgctgag cagccgggta aaatcgccgg cgcaatctaa gggatctgaa
     3061 aggcatggtc aaagcacgcc gatgcggctg gtgcggcgac gacccgctct acgttgccta
     3121 ccacgaccgc gagtggggca tgccggaacg ggatcccacg gtccttttcg agcggctggt
     3181 gctcgagggg atgcaggcag gcctttcgtg gctcacgatt ctcaacaaac gcgaaaacat
     3241 gcgcgagcgc ttcttcgcat tcgaccctgc tcgcctcgca gcagccggcg aggccgaggt
     3301 ggcggcgtgg ctgaacgacc cgggtatcat ccggcatcgc ggcaagctcg aggcgatgat
     3361 ctccaacgcg cgggcctatc tgcggcaggg agacttctcc aatctgctgt ggtctgcggt
     3421 tggcggcgcg ccgcggcaga atcggtggcg gcatctgggc gatgtaccca gccagaccgg
     3481 ggagtcccgg gatcttgccc ggatgctgaa gtccgccggc ttcggcttcg tggggcccac
     3541 cacctgctac gcgttcatgc agagtgcggg gctggtcaac gaccacctgg tcagctgccc
     3601 cgcgcatcaa aggtgcgcag atgcctggac ggccctttgc ggcggctgaa gggctggctc
     3661 atccttgccc tgctgcgggc aacgagcatc ctgccgttgg cggcggcccg ggcgctggca
     3721 agggccgctg ccgcggccgc cctgacactc gacgtccgcg ccgcccgcac gacccgaatc
     3781 aacgtcgcgc tttgctttcc ggagcttggt cccgaagagc aggctcggct ggtgcgcgat
     3841 agcctcgcgc actcggcaag cgtcgtcgcg gaggccggac cgctttacca ttggccccgg
     3901 gcgcgttggc aaacccgctt gacgctcgtg ggagatgagc tattcaacga agcaatggcg
     3961 tccggtcgag gcgttctgct gctcggtccc catttcggca actgggaggc gctgcctctg
     4021 tttctcggcc gctacgcggt caccgggctc tacgacccgc cccgaatctc cgctctggaa
     4081 aaaaccgtga gggctgccag ggaaagaaac ggcatgcgat tgctgcccat cgatgcccgt
     4141 gggctgcgcg gggtgtatca ggcgctcagc gccggctccg ccgcgggcat tcttcccgac
     4201 caggttccgg gccggaacgc gggtgtgtac gcccccttct ttggtcaccc cgcgctgacc
     4261 atgaccctgg ctcaccggct catccggcgg acccggcccg tcgtgctgct tgtcgcggcc
     4321 agacgggtaa gcggcggctt ccggatcgaa tttcacgccg cgcccccgga catcctcgcg
     4381 ccggatcccg agacttcagc ggctgccatg aactcggcca tcgagtcgct cgtccgcacc
     4441 gacccgtccc agtaccagtg ggagtacaag cgattcaggc ggcaacccgc aggagttgcg
     4501 gatccctatc gggtcgggta accgggcgct tcagcggcgc cagaaggctg gggtcatcag
     4561 aacgagcagc gtgaagatct ccagtcggcc cagcagcatc gcgaagatca gcaaccactt
     4621 caccgatacg gggagcgcgg cgtagttgag cgccacctcg ccgaggccgg gccccaggtt
     4681 attcaggcag gcgcccaccg ccgagaaggc ggtgaggaaa tccagatcgg agatcagcat
     4741 caggcaggcg accatggcca gaaagatgat cacgtaaaac gaaaagaacc cccagacggc
     4801 gtcgacgaca cggtcgggga cgcgatgtcg gcccagcttg atggggaata cgccctgggg
     4861 gtgtatgagc cggcggatct cgcgaatgcc ctggcggtag atgagcaaca cgcggatcat
     4921 tttgaccccg cccgccgtcg agccggcgca ccctccggcg aacgcggcga acatcagcag
     4981 gacgggcgcg gccgagggcc agacgctgaa gtc
//
LOCUS       DBNHLM010000155        16339 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_381373, whole genome shotgun sequence.
ACCESSION   DBNHLM010000155 DBNHLM010000000
VERSION     DBNHLM010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 16339)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16339)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16339
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_381373"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="AC16QF_07140"
     CDS             complement(<1..1066)
                     /locus_tag="AC16QF_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR6448367.1"
                     /translation="MAAAPGYVRWFSEIASADLALVGGKNASLGEMYQELTSEGLRVP
                     NGFALTTRLYRDALAEAGIDGALREVLDALDVNDVEALQLGGQRCRELVYDAGLPVGS
                     REQILGAFAELRAQYGADLRVAVRSSATAEDLPHASFAGQHDSYLNISGGEALIDACR
                     RCLASVFTDRAIRYRADHGFDHLEVALSVGIMKMVRADRGSSGVIFTLDTESGFRDVI
                     LINSSWGLGENIVQGTVDPDEFYVHKPTLRQGHRCVLRRKLGEKQMRMLSGSRRGGGS
                     TYNVPTDPADRARYSLSDEDVLALADQALIIEAHYSRRAGADVPMDIEWARDAEDGEL
                     YIVQARPETVASRANPNRLVH"
     gene            1189..2310
                     /gene="ald"
                     /locus_tag="AC16QF_07145"
     CDS             1189..2310
                     /gene="ald"
                     /locus_tag="AC16QF_07145"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR6448368.1"
                     /translation="MRIGVPREIKADEYRVGMTPASVRETVEHGHEVFVQSLAGEAVG
                     LHDADYQEAGARVVQDAEAVFESAELIVKVKEPQPEEAALLRPDHILFTYLHLAPNPS
                     LTLALSESGCTAIAYETVTDTQGGLPLLTPMSAVAGRMAVQAGAHCLEIEQGGRGLLL
                     GGVPGTPPARVTVLGAGVVGSNAARVALGMEADVTILDTSLRKLAELDLHFGPALNTI
                     FSTADAVQEFVLDADLVVGAVLVPGSETPKLISEAMVTEMRKGSVLVDVAVDQGGCAA
                     TSRPTTHSNPTYTVDDVVHYCVANMPGAVARSSTFALNNATLPFVLAIADKGAVTAMR
                     DDAHLRHGLNVCAGRVTYAAVASSAGLPFAEAEDVLRNP"
     gene            complement(2307..2519)
                     /locus_tag="AC16QF_07150"
     CDS             complement(2307..2519)
                     /locus_tag="AC16QF_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448369.1"
                     /translation="MGENDDVVAACGGCHQGPLALDGFGADDLASRLRRIRDGDTPHP
                     PLNLEADDDHALEDLARRILAAGSDG"
     gene            complement(2628..3056)
                     /locus_tag="AC16QF_07155"
     CDS             complement(2628..3056)
                     /locus_tag="AC16QF_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6448370.1"
                     /translation="MSIEPQRVRDYMSRELLTVSPETDIMRAIHLLVEHDISGLPVVD
                     AQDGLCGILTERDCIRIAVQAGYFDEAGGRVADYMSPSVHTVTPDDSLMDLAELFVES
                     PFRRCPVVEDGRLVGLICRRDVLRALKGGAWFADAGEHPR"
     gene            complement(3078..4301)
                     /gene="nqrF"
                     /locus_tag="AC16QF_07160"
     CDS             complement(3078..4301)
                     /gene="nqrF"
                     /locus_tag="AC16QF_07160"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HIR6448371.1"
                     /translation="MLEIVVPAVALTTIVFLLSLAVLLVRRGLVPGEDVEILINDQRA
                     LRGDAGEKLLWALADHDVYLPAACGGRGSCGQCRIEVTRGGGAITPAEENHIARRDAA
                     RGVRLACMVTLGDDVAVHLPPELLEIRRWSSKVLANRHVTTYLKELTLELPPGLELNF
                     NAGAYVQLEAPAGIVRFDGFDIAAEYDAEWQRHGLRDLVVEIPETTTRAYSLASHPGE
                     SGAIKLVVRIALPPPDAPVGTPPGRVSSYAFGLKPGDEVWLAGPFGEFRARDTDREMV
                     LIGGGAGIAPLRAIVLDQLLNKRSGRKISFWYGARNERELCYHSELTELARQHENFAY
                     RVALSDPAPDSAWQGLTGLVHAVVYREYLEGHPSPEEAEYYLCGPPLMSSAVLAILED
                     LGVDRDSVFFDDFGS"
     gene            complement(4304..4912)
                     /gene="nqrE"
                     /locus_tag="AC16QF_07165"
     CDS             complement(4304..4912)
                     /gene="nqrE"
                     /locus_tag="AC16QF_07165"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HIR6448372.1"
                     /translation="MEHLLNIVVRAIFVENLALVFLLGMCTYIAVSKRVETAIGLGVA
                     VVVVQLITVPVNNLLYEYVLTPGALAWMGLGTLDLSFLKLITFIGVIAALVQMLELVI
                     ARFAPQLQQHLGIYLPLLTVNCAILGASLLMVQRSYDFAESVAYGLGVGIGWAMAIVL
                     FAAIRERLKYSDVPKGLEGLGLAFIVTGFLAFGFIGFTGVEF"
     gene            complement(4912..5562)
                     /locus_tag="AC16QF_07170"
     CDS             complement(4912..5562)
                     /locus_tag="AC16QF_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HIR6448373.1"
                     /translation="MLRHLTHPLVSENPITVQILGLCSALAVSSTLKPAFIMSVSVVA
                     VLVFSNVSVSLLRNVFPRSIRLILEVTLIASAVIVVDEVLKAFAPETADILTVFVGLI
                     VTNCIILGRAEAFAMHHRVAPSLADALGNGLGYALVLLAVATARELFGHGTLLDVQIL
                     PLSEAEGGWYRPNQMMRFAPSAFFLIGLIIWGVRSVRAEQVEAGAYPPASPSYREG"
     gene            complement(5579..6298)
                     /gene="nqrC"
                     /locus_tag="AC16QF_07175"
     CDS             complement(5579..6298)
                     /gene="nqrC"
                     /locus_tag="AC16QF_07175"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HIR6448374.1"
                     /translation="MAHEPISRTLAVAVAIALVCSVMVSTAVYLLRPIQIAYDLIDRN
                     RAIVAAAGIEGVLQGGDDAAVVRAFLDLDARVLDLDSGEFLDTTDAHGYDHWAGTTDD
                     ARLVPVYVSRRDGAATRLVLPVHGRGMWSTIYGYVALDADLTTVAALVIHRHGETPGV
                     GDRIQDPAWRAKWRGKRAFGPDGAVAIHVARGGNGQQPGRVDTITGATVTSEAVGQLV
                     AGWLAIYSPWLSRIAAPGARI"
     gene            complement(6301..7446)
                     /locus_tag="AC16QF_07180"
     CDS             complement(6301..7446)
                     /locus_tag="AC16QF_07180"
                     /inference="COORDINATES: protein motif:HMM:NF015100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR6448375.1"
                     /translation="MADSTILQRVRGIVIDLADPYRAPEARAAGSPPFLRQALDTPGL
                     LACFVVATLPAWAVGVWNTGRRGLEAGFGSEGESWQLGMLAAFRVDADPTAMLACLTL
                     GVLYFIPLLAAVLATSAFWALVFARTRQGGVDPGWLFSGWLMVLLMPPSTPLTIAVLA
                     ASFGVVLGSHIFGGTGKYLISPALLGVLFLHFSYPELVQTPLAAGDAGSADGWRALAA
                     GGEDVVEFLLGREPGGLATGSALACAVGALYLVRVGAASWRTIAGAVVGFLVAATVVN
                     VSAGEDPAWHVPWYAHLAVGYVAFGVAFLATDPSAAPLTRPARWAHGLLIGVLTVVIR
                     VLDPTHPEGSLYALLLAGLSVPFLDYLVVRRYRTRVRIRAQLRTRGL"
     gene            complement(7446..8717)
                     /locus_tag="AC16QF_07185"
     CDS             complement(7446..8717)
                     /locus_tag="AC16QF_07185"
                     /inference="COORDINATES: protein motif:HMM:NF048463.2"
                     /note="uses the energy from reduction of ubiquinone-1 to
                     ubiquinol to move Na(+) ions from the cytoplasm to the
                     periplasm; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit A"
                     /protein_id="HIR6448376.1"
                     /translation="MLIEITTGLDVEVSGRPEQTIDTGPEVGQVALLGGDYLNLRPSL
                     EVDEGARVKAGDTIFSDRARPEIRFVAPAAGRITQINRGPRRTLVSVVIDVDGDERAI
                     VDVPDDPSRREEIVALLLRSGLWPALRSRPFGRVADPGGTPAALLLTAIDTRPLAPDP
                     AVVVGLREADFAAGARALACLAPVTYLCSRAGASLPDVDAEGLSVVEFSGPHPAGLVG
                     THLRHLCPEADEVWHIGYQDVLALGRLLVSGELCDERVVSVAGPGAVRPRLLRTRAGA
                     RLSELVQGESENGCAVIAGSVLSGRAPTPASGYLGRYDSQVSLLRAAGPSRRRWLDVV
                     WPRGAAPARNIGRGSGMLPVESFDRVWPFQAPVAPLLRALLTGDTELASSLDCLSLVE
                     EDLALCAYVCPSRLDYGAALRATLHEIERGR"
     gene            8807..9655
                     /locus_tag="AC16QF_07190"
     CDS             8807..9655
                     /locus_tag="AC16QF_07190"
                     /inference="COORDINATES: protein motif:HMM:NF025890.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR6448377.1"
                     /translation="MRLFSMAVGTLFLLATGQVGGNPTCAACHGELVADFHGTPHGAR
                     TEDDEASGCRSCHGDPATHLAAPAEPGSLLAFDDEPSATINAACGSCHADAHTARANP
                     HSQAGLACTDCHSIHPSSAAEIALPAEFDRVDAASATCFECHQETFSQFSFNERHRLQ
                     EGSVSCTSCHDPHAPASSGHLGAFGQRVCAECHADVDGPFVFEHDASRVEGCGACHEP
                     HGSPNRHMLTHQQVGELCYSCHAIVPQFHLGFSPAGPPRFDEGTVCTNCHATIHGSNL
                     DPAFLR"
     gene            9663..11522
                     /locus_tag="AC16QF_07195"
     CDS             9663..11522
                     /locus_tag="AC16QF_07195"
                     /inference="COORDINATES: protein motif:HMM:NF023282.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR6448378.1"
                     /translation="MTARLLIVFTLLIAGAASTRSAAAATFTGHVSVGYREVDVSGDE
                     GKYQQHVNLDDGARLFDLGMVFQPDRDANSPAGMPDRVELNASGLGGDPYESVRVRVQ
                     RYGSYRLTYDHRSSDYFYEDLLLAPEEASVEQSTGGDFHHFDFERNRDALALDLDLSS
                     RAKLNLGFARYEKRGESTTVLDVEREEFELDKPIDESLDRYELGFTYAWDHVTVALGQ
                     TWQEYDNDSQIFLPGFSPGSEADAPTSLDLFFLDQPYGFTSSDQRLSVQVRPSSQWIV
                     ELDARAGNLDLDMTATERAQGTDYLGRPFDREDAGAADVDRDTLLLDASVAYLVNDRL
                     QLNAGVRRYDLEQDGAVLFGDTAGASAWDIETTGFELGVDMAVTTTVTLSGGWSTERR
                     KSVVSHAIEGAGHTEAVDTDRDGFFGRLSYRPSRRAQVTLSFEDDRIDDPYTLASATD
                     ARRYKARASYRWDNGLKVSASYRRTDYENDNTGWISESRQSDIRLTHAGDRLTVSVGA
                     AFVELQREVDQLVTGGSRQDLFAISYGADADFWDGAVRWHVTDRVDLTASYRAYENEG
                     SFRVDRDDVRAGVEFLLPADYALLLSYRNVDYNEDELESFDADVWELALKFRW"
     gene            11548..12624
                     /gene="pdhA"
                     /locus_tag="AC16QF_07200"
     CDS             11548..12624
                     /gene="pdhA"
                     /locus_tag="AC16QF_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HIR6448379.1"
                     /translation="MSDSLPFGVHPSTIIGPDGNLLRPIPEPLVDPATIRALYGWMHR
                     TRAFDAKAIELQRTGRLGTYASCLGQEAASVGIGHAMAPEDVLVPSFRDQGTQLYRGV
                     SMVELLLYWGGDERGSDYAVPRDDLPISITVGGHAPHAAGVALALKLRAEPRVAVCVF
                     GDGATSKGDVYEAMNIAGVWRLPVVFVVNNNQWAISTPRADQSAATTLAQKAVAAGFE
                     GSQVDGNDVLAVSEAVGDAIGHARKGDGPRLVECLTYRLGDHTTVDDASRYRSEAEVA
                     RWRQLDPLARVGAYLAQHEGWSDADEDALVERCDDEVASAATAYNGTTPQPPEAIFDH
                     LFASLPRELERQRATLLASAPADG"
     gene            12617..13597
                     /locus_tag="AC16QF_07205"
     CDS             12617..13597
                     /locus_tag="AC16QF_07205"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR6448380.1"
                     /translation="MAEHTLLEAVNLALRRAMADDDRVLVLGEDVGVVGGVFRATDGL
                     LQAFGPHRVFDTPLAETGIAGVAVGLAVQGFRPVAEIQFMGFIYAAIDQLANHASRMR
                     TRTRGRQSCPLVLRAPFGGGIHAPEHHSESAEAMLAHIPGLRVVIPSSPRTAYGLLLA
                     AIRDPDPVVFLEPKRLYRSAKEAFEDDGAALPLDRCHIVRPGKDLTLVTWGAMVQETL
                     AAAEQLSGEGVAAEVIDVATIKPLDGETICESVGRTGRLVIVHEAPITGGFGGEIAAC
                     VAERALYSLQAPILRVAGYDTVMPLARLEHHYMPSVSRIVGAARETLEAS"
     gene            13594..14700
                     /locus_tag="AC16QF_07210"
     CDS             13594..14700
                     /locus_tag="AC16QF_07210"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR6448381.1"
                     /translation="MIATFDNVDELGPEKVIEIYDPSTGLKAIVVVDNVAAGPAIGGV
                     RMAVDATREECGRLARAMTLKNAMAGLAHGGAKSVIVADPAMADEAKEVLIRSFAHAI
                     ADLRDYIPGPDMGTDETSMALVRDVTGRSAGLPRELGGIPLDEIGATGLGLSVSLEVA
                     EAFTDLSLDGARIVVQGFGSVGQHAARFLARRGARLVGVSDSRGARAAAEGFDLDLLL
                     ASKQEGVSVGAFDEGEAIDPEDLVELDCDVWIPAARPDVLNGANAGRIRAKVVAQGAN
                     IPATPEAEQTLAARGVLVIPDFVANAGGVICAAVEYHGGSESQALATIQDKIHGNVKE
                     VLSRATTTGELPRKVAMDIARERVQAAMRYRKTY"
     gene            complement(14762..15847)
                     /locus_tag="AC16QF_07215"
     CDS             complement(14762..15847)
                     /locus_tag="AC16QF_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6448382.1"
                     /translation="MDIGICVASHIGDLDYVVRAEALGYSHAWLADSQMLWSDCYATL
                     ALAADRTQKIRLGTGVAVSGTRPAPVNAAAIATINAIAPGRTFLGVGAGNTAMRVMGQ
                     PPHRIAEFDRYLSTLVPLVRGEEAQMGPLTVGARGEAPIRHIMPDKGFVNFDDRIPVY
                     VSGFGPRSLGLAGKHGDGAVLAMPANPAVMANIWRMLEEGAAAAGRTLDRSDFYTTGL
                     TAIVVLDPGEASDSPRVRAECGAMAMASVHYAYDQWRNFGHQPPGYLGGIWDDYCALL
                     ESYPQERRHQRIHAGHNCWVLPEEERFLTREVLETTCMIGTRDQLIDRLAALSEAGLD
                     QVMILPNFDTRFDVLERVGRELVGRIP"
     gene            16001..>16339
                     /locus_tag="AC16QF_07220"
     CDS             16001..>16339
                     /locus_tag="AC16QF_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448383.1"
                     /translation="MGQLKFDHLQPIVDDDATIEAALADAHIPSLMAAMVHVSGDPSW
                     IRGAIRPVNQFFGDSQGGIAPNDQAEVRRRALEALIAYRDGKATPPPPDPATVSEMVN
                     FVIGQEVTSDY"
BASE COUNT         2937 a         5589 c         5347 g         2466 t
ORIGIN      
        1 aatgcacaag ccggttcggg ttggcgcgcg aagccacggt ttccggtctt gcctgcacga
       61 tgtagagctc gccgtcctcg gcgtccctgg cccactcgat gtccatgggc acgtccgcac
      121 ccgcacggcg cgagtaatgc gcttcgatga tcagggcctg gtcggcaagg gcgagcacgt
      181 cctcgtcgga gagcgagtac cgcgcgcgat cggccggatc cgtcggcacg ttgtaggtcg
      241 atcccccacc ccgccggctc cccgacaaca tgcgcatctg cttctcgccg agcttccggc
      301 gcaggacgca tcggtgcccc tggcgcaggg tgggcttgtg gacatagaac tcgtcgggat
      361 cgacggttcc ctggacgatg ttctcgccca ggccccagga ggaattgatg agtatgacgt
      421 cgcgaaaccc cgactcggta tccagggtga agatcacccc gctgctgccc cgatcggcgc
      481 ggaccatctt catgatccct acggacaggg ccacctccag atgatcgaag ccgtggtctg
      541 cccggtaccg gatggcgcga tcggtgaaaa cgctggccag acagcgccga caggcgtcga
      601 taagcgcttc gccccccgag atgttgaggt aggaatcatg ctgaccggcg aagctggcgt
      661 ggggtaggtc ctcggcggtg gcggagcttc tcaccgccac ccgcaggtcc gccccgtact
      721 gagctctcag ctcggcgaac gcgccgagaa tctgctcccg gctccccacc ggcagcccgg
      781 cgtcgtagac gagctcgcgg cagcgctgac cgccgagctg cagcgcttcc acgtcgttga
      841 cgtccagggc gtcgagcacc tcgcgcagag cgccgtcgat cccggcctcg gcaagcgcgt
      901 cccgatacag cctcgtggtc agggcaaacc cgttgggcac acgaagcccc tcgctggtga
      961 gctcctgata catctcgccg agcgacgcgt tcttgccgcc caccagggca agatcggccg
     1021 aggcgatttc cgagaaccat cgaacataac cgggcgctgc cgccatgagc cctccctcgc
     1081 attgccgttt ctgctccctc aggccccgac gttatctagg ggtaattccg taatcaatgg
     1141 cctttcgcgg acgtgtacaa acgcaccacg cacaaaaagg tgaaagacat gcgaatcggt
     1201 gtgcccagag agatcaaggc cgacgagtac cgggtgggca tgacaccggc gagcgtccgg
     1261 gaaaccgtcg agcacggaca cgaggtattc gtgcagtccc tggcaggcga agccgttggc
     1321 ctgcacgacg ccgactatca ggaggcgggc gccagggtgg tccaggacgc cgaggcggtg
     1381 ttcgaatcag ccgagctcat cgtcaaggtg aaggagcccc agccggagga agccgcgctg
     1441 ctgcgcccgg accacatcct cttcacctac ctgcatctgg ctcccaaccc ttcgctcacc
     1501 ctggcgctga gcgaatccgg atgcacggcg attgcttacg agacggtcac cgacacccag
     1561 ggcggcttgc ccctgctgac gcccatgagc gccgtggccg gacgcatggc cgttcaggct
     1621 ggcgcccact gtctggaaat cgagcagggc ggccgcggtc tcctgcttgg cggcgtgccc
     1681 ggcacgccgc cggccagggt caccgtgctg ggcgccggcg tcgtcggcag caacgcggcg
     1741 cgggtggccc tgggcatgga ggccgacgtg accattctcg acacctcgct gcgcaagctc
     1801 gccgagctcg acctgcattt cggacccgcg ctcaacacca tcttctcgac agcggacgcc
     1861 gtgcaggagt tcgtgctgga tgcggacctg gtggtagggg ccgtgctggt gcccggcagc
     1921 gagacgccga agctgatctc ggaagccatg gtgaccgaga tgcgcaaggg ctcggtgctg
     1981 gtggacgtcg ccgtcgacca gggcggttgc gcggccacct ctcggcccac cacgcattcg
     2041 aaccctacct acaccgtcga cgacgtcgtt cactactgcg tcgcaaacat gcccggcgcg
     2101 gtcgcccgct cgtccacctt cgcgctcaac aacgccaccc tgccattcgt gctggccatc
     2161 gctgacaaag gcgcggtgac cgccatgcgc gatgacgctc acctgcgtca cgggctgaac
     2221 gtttgcgcag ggagggtcac gtacgcggcg gtcgccagct cggcgggcct tcctttcgcc
     2281 gaggcggagg acgttctgcg gaatccttag ccgtcgctgc cggctgccag gatccgccgc
     2341 gcgaggtcct cgagggcgtg atcgtcgtct gcttcgaggt tcaggggggg atggggcgtg
     2401 tcgccgtcac ggatacgcct gagtcggctc gcgaggtcgt cggctccgaa gccgtcaagg
     2461 gcaagcgggc cctgatgaca gccaccgcag gctgccacga cgtcgtcgtt ctctcccatc
     2521 gccgtgcctg cctctaggca aaaggaaaca ctcagcatgc cggcggacaa taacgcgcgt
     2581 cttcgattca acctcgaccc cttcccggaa actgcggagc tgcaatccta ccgtgggtgc
     2641 tcaccggcgt cagcaaacca ggcgccgcct ttaagggcgc gcagaacgtc gcgccggcag
     2701 atgagcccca ccagccggcc atcctccacc accgggcagc gtcgaaaagg cgactccaca
     2761 aaaagctcgg cgaggtccat caggctgtcg tcgggcgtga ccgtgtggac cgaaggggac
     2821 atgtagtccg ccacccggcc gcccgcctcg tcgaaatacc cggcctgaac cgctatgcgg
     2881 atgcaatccc gctccgtcag tatcccgcag aggccgtcct gcgcgtccac caccggcagt
     2941 cccgaaatgt cgtgctcgac cagcagatga atggcccgca tgatgtcggt ctcgggcgag
     3001 acggtcagaa gctcccgcga catgtagtcc cttacccgtt gaggttcgat gctcatgatc
     3061 atgcctcctt tgtccgatca ggatccgaag tcgtcgaaga acacgctgtc gcggtccaca
     3121 ccgaggtctt cgagaatggc caggaccgcg ctgctcatca gcggcggccc gcagaggtag
     3181 tactcggctt cctcgggaga cggatgcccc tccaggtatt ccctgtacac gacggcgtgc
     3241 acgaggccgg tcagtccctg ccaggcggag tccggcgctg gatccgacag cgccacgcgg
     3301 taggcaaaat tctcgtgctg gcgggcgagc tcggtcagct ccgagtggta gcagagctcc
     3361 cgctcgttgc gggcgccgta ccagaaactg atctttcgcc ccgaccgctt gttgagcagc
     3421 tggtcgagca cgatggcgcg cagcggcgcg atgcccgcgc cgccgccgat gagaaccatt
     3481 tcccggtcgg tatcccgcgc gcgaaactcg ccgaacgggc ccgccaacca gacttcgtca
     3541 ccgggcttca ggccaaacgc gtaggacgag acccgcccgg gcggggtccc caccggggca
     3601 tccgggggcg gcagggcgat gcgaacgacc agcttgatgg cgccggattc gccggggtgg
     3661 cttgcaagag aatacgcgcg ggtggtcgtc tccggaattt ccacgacgag atcccgaagg
     3721 ccgtgccgtt gccattcggc gtcgtactcg gcggcgatgt cgaagccgtc gaaccgcacg
     3781 atgccggccg gggcctcgag ctgcacgtag gcgccggcgt tgaaattcaa ctccaggcca
     3841 ggcggcagct cgagggtgag ctccttcaag taggtggtca cgtgacggtt tgccagaacc
     3901 ttcgagctcc agcggcggat ctccagcaac tcgggaggca gatgcacggc gacgtcgtcg
     3961 ccaagggtga ccatgcaggc gaggcgcacg ccgcgagcgg catcacggcg cgcaatgtgg
     4021 ttctcttccg ccggcgtgat ggcgccaccg ccccgcgtca cctcgatgcg acattgcccg
     4081 cagctgcccc ggccaccgca ggccgccgga agatagacat cgtggtcggc cagcgcccac
     4141 aggagcttct cgccggcgtc gccccgcaga gcccgctgat cgttgatcag aatctccacg
     4201 tcctcgccgg gcaccaggcc gcggcggacg aggagcaccg ccagcgagag caggaagacg
     4261 atggtcgtca gcgcgacggc tggcacgacg atctccagca cgtctagaac tccactccgg
     4321 tgaagccgat gaagccgaag gcgaggaacc cggtcacgat gaaggccagg ccaaggcctt
     4381 cgagcccctt cggcacatcg ctgtacttca agcgctctcg gatggcggca aagagaacga
     4441 tggccatcgc ccagccgatg ccgacgccga gcccgtaagc gacgctttcg gcgaagtcat
     4501 agctgcgctg caccatgagc agggaagcgc cgagtatggc gcagttgacg gtgagcagcg
     4561 gcaggtagat gcccaggtgc tgctgcagct gcggcgcgaa ccgcgcgatg acgagctcca
     4621 gcatctggac aagagcggcg atgacgccga tgaaggtgat cagcttgagg aacgaaagat
     4681 ccagggttcc cagccccatc caggccagcg ccccgggagt gaggacgtat tcgtagagga
     4741 ggttgttgac gggcacggtg atgagctgca cgacgacgac ggcgacgccg agcccgatgg
     4801 cggtctccac acgcttggag accgcgatgt acgtgcacat gccgagcagg aacaccaaag
     4861 ccaggttctc gacgaagatg gcgcgcacaa caatgttcag gagatgctcc atcagccctc
     4921 ccggtagctc gggctcgccg gcgggtaagc tccggcttcg acctgttccg cgcgcacgct
     4981 gcgaacgccc cagatgatga gcccgatcag aaaaaaagcg ctgggcgcaa agcgcatcat
     5041 ctggttgggg cgataccaac caccttccgc ctcgctcagg ggcagtatct gaacgtcgag
     5101 caacgtcccg tgcccgaaca gctcgcgagc cgtggcgacg gccagcaaca ccagcgcgta
     5161 gcccagacca ttcccaaggg cgtcggcaag actgggggcc acccggtggt gcatggcaaa
     5221 cgcttcggcc cgtccgagaa tgatgcagtt ggtgacaatc aggccaacga acacggtcag
     5281 tatgtcagcc gtctccggag cgaaggcctt gagcacctcg tccaccacga tcaccgccga
     5341 ggcgatgagc gtcacttcca ggatcagacg aatgctgcgg ggaaagacgt ttctcaacag
     5401 gctgacggac acattcgaga aaaccagcac cgcgacaacg gaaacgctca tgatgaaagc
     5461 cggcttgagg gtgctggaca ccgcaagggc ggagcagaga ccgaggatct gcacggtgat
     5521 cgggttctcg ctgacgagcg gatgggtgag atgcctcaac atgaatggtg tccacgcttc
     5581 atattcgtgc tccaggcgcc gcgatccggg aaagccaggg cgagtagatg gccaaccagc
     5641 cggcgaccag ctggcccacg gcttcgctcg tcacggtcgc gccagtgatc gtatccaccc
     5701 tgcccggctg ctgcccattg cccccgcggg cgacgtgaat cgcgaccgcg ccatcgggcc
     5761 cgaatgctcg tttgccacgc cacttcgccc gccaggcggg atcctgaatc ctgtcgccca
     5821 cgcccggcgt ctcaccatga cgatggatga ccagagcagc gacggtcgtc agatccgcat
     5881 ccagggccac gtagccgtat atggtcgacc acatccctcg accatgaacc ggaagtacca
     5941 gccgggtcgc tgcgccgtct ctcctcgaga cgtatacggg aacgagccgc gcgtcgtccg
     6001 tcgtgccggc ccagtggtca tagccgtgag cgtcggtcgt atccaagaac tcgccgctgt
     6061 cgagatcgag cacccgggca tccaggtcga gaaacgctcg aacgacggcg gcatcgtccc
     6121 cgccctgcag cacgccctcg ataccggcgg ccgcgacgat tgcgcggttg cgatcgatga
     6181 ggtcgtaggc gatctgaatc ggccgaagca ggtagacggc ggtagaaacc atgaccgagc
     6241 agaccagcgc tatggccaca gcgacagcca gcgtgcggga aatcggttcg tgcgccacga
     6301 tcagaggccc cgcgtccgca gctgcgcccg aatccgcacc cgcgtgcggt acctccgcac
     6361 caccaggtag tccagaaacg gcacgctgag ccccgccagc aggagcgcgt acagagagcc
     6421 ttccgggtgg gtcggatcca gcacgcggat cactaccgtg agcacgccga tcagcagacc
     6481 gtgcgcccac cgggccggcc gggtcagtgg cgcagcgctg ggatcggtgg ccagaaaggc
     6541 aaccccgaaa gccacgtagc cgaccgccag atgagcgtac cagggcacat gccaggcggg
     6601 gtcttccccc gccgagacgt tgacgacggt ggccgcgacg aggaagccca cgacggcgcc
     6661 cgcgatcgtg cgccaggagg cggcgcctac gcgcaccaga tagagcgctc cgacggcgca
     6721 ggcgagcgcg gagccggtcg ccaggcctcc cggctcgcgc cccaggagga attcgacgac
     6781 gtcctcgccg cccgcggcca gcgcgcgcca cccgtctgcg ctccccgcat cgccagcggc
     6841 gagcggggtc tgtaccagct cgggatagct gaagtgcagg aacagaacgc cgagcagggc
     6901 cgggctgatc aggtatttcc ccgtcccgcc aaagatgtga ctaccgagca ccacgccaaa
     6961 gctcgccgcc aggaccgcga tggtcagcgg ggtcgacggc ggcatgagca gcaccatgag
     7021 ccacccgctg aacagccagc cagggtcgac gccgccctgc cgggttctcg caaaaaccag
     7081 ggcccagaag gcactggtgg ccaggacggc cgcaagtagc gggatgaaat acagcacccc
     7141 gagcgtcagg caggccagca tggccgttgg atccgcatcg acgcggaacg cggccagcat
     7201 gccaagctgc cagctttcgc cctcgctccc gaaccctgct tccagtccgc gccgaccggt
     7261 gttccagaca ccgacggccc aggccggcag ggtagccact acgaagcagg cgagcagtcc
     7321 cggagtgtcc agggcctggc gcagaaaggg cggcgatccc gccgcgcggg cttcaggtgc
     7381 gcggtaggga tccgcgaggt cgatcacgat tcccctgacc cgctgaagga tcgtcgagtc
     7441 agccatcagc ggccccgctc gatctcgtgc agggtcgcac gcagcgcggc cccgtagtct
     7501 agtcgcgatg ggcacacgta tgcgcacagg gccaggtctt cctcgaccag agacaggcaa
     7561 tccagggagc tcgcgagctc ggtgtcgccg gtgagcagcg cacgaaggag cggcgcgacg
     7621 ggggcctgga acggccagac gcggtcgaag ctctcgacgg gtagcatccc gctcccacga
     7681 ccgatgttcc tcgccggagc ggccccgcgc ggccaaacca catccagcca ccggcgtcgt
     7741 gacggacctg ccgcgcgcag cagcgatacc tgagaatcgt accggccaag atacccactc
     7801 gccggcgtcg gtgcgcgtcc gctcagaacg gacccggcga tcacggcaca gccgttttcc
     7861 gactcgccct gcacaagctc gctcaggcgc gcacccgccc gcgtgcgcag cagccgcggc
     7921 ctgaccgctc ccggcccggc cactgaaacc actcgttcat cgcaaagctc gcccgagacg
     7981 agcaatcgtc cgagtgccag aacgtcctga tacccgatgt gccagacctc gtccgcctcc
     8041 gggcacaggt ggcggagatg ggtacccacc aggccggccg gatgcgggcc gctgaactcc
     8101 accacgctca agccctcggc atcgacgtcc gggaggctgg cccctgcccg ggagcagaga
     8161 taggtgaccg gcgccagaca ggcgagcgcc cgggcgccgg cggcaaagtc agcctctcga
     8221 agcccgacga ccacggccgg atccggggcc aggggccggg tgtcgatggc cgtgaggagc
     8281 agcgcggccg gtgtccctcc gggatcggcc acgcgaccga agggtcgcga tctgagggcg
     8341 ggccaaaggc ccgaacgcag gagcagcgcg acgatctcct cgcgccgtga cgggtcatcc
     8401 ggcacatcaa cgatcgcccg ctcatcccca tcgacgtcga tcaccaccga gaccagcgtg
     8461 cgccgtggtc cacgattgat ctgggtgatc cggccggcgg ctggtgctac gaagcgaatt
     8521 tccggacgcg cccggtcgct gaagatcgta tcacccgctt tgacccgggc accttcgtcc
     8581 acctcgagcg acgggcgaag gttcaggtag tccccaccaa gcagggcaac ctggccgacc
     8641 tccggacccg tatcgatggt ctgctccggc cgaccggaga cttcgacgtc aaggcctgtg
     8701 gtgatctcga tcagcatgcc ggcaccaaac tgacacgacc ctcgggtagt atgtctacgt
     8761 agaactcaca atagcagagc gacacgtgct gctggaccct aaacccatgc gtttgttttc
     8821 aatggcggtg ggcactctgt ttctgctggc aaccggccag gtcggcggaa acccgacctg
     8881 cgccgcttgc cacggcgagc tcgtggcgga cttccacggt actccccacg gggcccggac
     8941 ggaggacgac gaagcgtccg gatgcaggag ttgccacggc gatccggcaa ctcacctcgc
     9001 cgcacccgcg gagccgggat ccctgctcgc attcgatgac gagcccagcg cgacgatcaa
     9061 tgccgcctgt ggcagctgtc acgcggacgc tcacaccgct cgcgccaacc cgcactccca
     9121 ggccgggctc gcttgcacgg attgccacag cattcaccca tcgagcgcag ccgaaatcgc
     9181 tctgcctgcc gagttcgatc gcgtcgacgc ggcctcggcc acgtgcttcg aatgccacca
     9241 ggagacattc agccagttct ccttcaacga gcgtcatcgc ctacaggaag gcagcgtttc
     9301 ctgcacgtcc tgccacgacc cccacgcgcc ggcgagctcc gggcatcttg gcgccttcgg
     9361 ccagagggtg tgcgccgagt gccacgccga cgtcgacggc ccgttcgtgt tcgagcatga
     9421 cgcgtcccgt gtggaaggat gcggcgcctg ccacgagccc cacggcagcc cgaatcggca
     9481 catgctcacg caccagcagg tgggcgagct ctgctactcg tgtcacgcca tcgtccccca
     9541 gttccacctc ggcttcagcc ccgcgggccc gccccgattc gacgaaggca ccgtgtgcac
     9601 gaactgccac gccaccattc acgggtccaa cctggacccg gcctttctga ggtgacgggc
     9661 tcatgacggc tcggctcctg atcgttttca cgctgctgat cgccggtgcg gcaagtaccc
     9721 gcagcgccgc cgccgcgacc ttcaccggtc acgtgagcgt cggctaccgc gaggtcgacg
     9781 tgtccgggga cgagggcaag taccaacagc acgtcaacct cgatgatggg gcccgcctgt
     9841 tcgatctcgg catggtcttc cagcccgacc gggacgcgaa tagcccggcc ggcatgcccg
     9901 accgggtaga gctcaacgcc agcgggctcg gcggggaccc ctacgaatcc gtgcgggtgc
     9961 gcgttcaacg ttacggaagc taccggctca cctatgatca tcgcagctcg gactatttct
    10021 acgaggacct gctgctcgca cccgaggaag ccagcgtcga gcagtccaca ggcggcgact
    10081 tccatcattt cgacttcgag agaaatcgcg acgccctcgc gctggatctg gatctgagca
    10141 gccgggccaa gctgaacctg ggattcgccc gatacgagaa acgcggcgag agcaccaccg
    10201 tgctggacgt ggagcgggag gaattcgagc tcgacaagcc catcgacgag tccctggaca
    10261 gatacgaact cggcttcact tacgcttggg atcacgtcac cgtggccctg ggccagacct
    10321 ggcaggagta cgacaacgac tcgcagatct ttctgcccgg gttctcgccg ggcagcgagg
    10381 cggacgcacc cacctcgctg gatctctttt ttctggacca gccctacggc ttcacgtcca
    10441 gcgatcagcg tctgagcgtg caggtgcggc catcatccca gtggatcgtg gagctcgacg
    10501 cgcgggccgg caacctggac ctggacatga cggcaacgga gcgggcccag ggcaccgact
    10561 acctcgggcg tccgttcgac cgcgaggacg cgggggcagc agacgtggac agggacaccc
    10621 tgctgctcga cgcctcggtc gcctacctgg tcaacgatcg tctccagctc aacgccggcg
    10681 tgcgccgcta cgatctggag caggacggcg cggtgctgtt cggcgacacc gccggcgcga
    10741 gcgcgtggga catcgagacc accgggttcg agctcggggt agacatggcc gtgacgacca
    10801 cggtgacact cagcggcgga tggtccaccg agcggcggaa gagcgtcgtc agccacgcca
    10861 tcgaaggcgc cggccacacc gaagccgtgg ataccgaccg ggacggcttc ttcgggcggc
    10921 tcagctaccg gccaagccga agagcccagg tcaccttgag cttcgaggac gaccggatcg
    10981 acgatcccta cacgctggca tcggctaccg atgcccgacg ctacaaggcg cgcgcatcct
    11041 accggtggga caacggcctg aaggtttccg cgtcctaccg gcgcacggac tacgaaaacg
    11101 acaatacggg ctggatctcc gaaagccgtc agtccgacat ccgcctgacc cacgcaggcg
    11161 accgcctgac ggtgtcggtg ggagccgcct tcgtcgagct ccaacgggaa gtggaccagc
    11221 tggtgaccgg gggctcccgg caggatctgt tcgccatctc ctacggcgcg gatgcggatt
    11281 tctgggacgg cgccgttcgt tggcatgtca ccgaccgggt ggacctgacc gccagctacc
    11341 gagcttacga gaacgagggc agcttcaggg tcgaccggga cgacgtccgc gcgggcgtcg
    11401 agtttctgct gcccgcggac tacgccctgc tgctcagcta ccgcaacgtc gactacaatg
    11461 aggatgagct tgaatctttc gatgccgacg tgtgggagct ggccctgaag ttccgctggt
    11521 gacgcgcacg acgtgccgga gccgaccatg agcgacagcc ttccattcgg agtccacccc
    11581 agcaccatca tcgggcccga cggcaatctc ctgcggccga tcccggagcc cctggtggac
    11641 ccggccacca tccgagccct ctacgggtgg atgcaccgca cccgcgcctt cgacgccaag
    11701 gccatcgagc tccagcggac cggacgcctg ggaacctacg cctcctgtct cggccaggag
    11761 gcggccagcg tcggcatcgg tcacgccatg gcgcccgagg acgtgctggt gccttcgttc
    11821 agagatcagg gcacccagct gtaccggggt gtgagcatgg tcgagctgct gctctactgg
    11881 ggcggcgacg agcgcggctc cgactacgcc gtgccgaggg acgatctgcc catcagcatt
    11941 accgtgggcg gccacgctcc ccatgcggcc ggcgtcgcgc tggcgctgaa gctccgagcc
    12001 gaaccccggg tggccgtgtg cgtgttcggc gacggcgcta cgtccaaggg cgatgtgtac
    12061 gaggcgatga acatcgccgg cgtctggcgg ctgcccgtcg tcttcgtcgt gaacaacaac
    12121 cagtgggcca tctccacgcc ccgagccgat cagtcggcgg ccaccaccct ggcgcagaaa
    12181 gcggtggccg ccggtttcga gggcagccag gtggacggca acgatgtgct tgccgtcagc
    12241 gaggcggtgg gcgacgccat cggccatgcc cgcaagggtg acgggccacg actggtcgaa
    12301 tgcctgacct accggctggg cgatcacacc accgtggacg acgccagccg ctaccgaagc
    12361 gaggccgagg tggcgcgctg gcggcagctg gatcccttgg cccgcgtggg tgcctatctc
    12421 gcccagcatg agggatggag cgacgccgac gaggatgccc tggtcgagcg gtgcgacgac
    12481 gaggtggcgt cggcagccac cgcttacaac gggacgacgc cccagccacc cgaggccatc
    12541 ttcgaccatc tcttcgccag ccttccccgg gagttggaac gacagagagc gaccctgcta
    12601 gcaagtgccc cggccgatgg ctgagcacac cctgctggag gcggtgaacc tggcgttgcg
    12661 ccgcgccatg gccgatgacg accgtgttct cgttctcggc gaggacgttg gggtggtggg
    12721 cggcgtgttc agggcaacgg acggcctgct gcaggccttc gggccgcacc gggtcttcga
    12781 tacccccctg gccgagaccg gcatcgccgg cgtggccgtc ggcctcgccg tgcaggggtt
    12841 ccggcccgtg gcggagatcc agttcatggg gttcatctac gccgcgatcg atcagctcgc
    12901 gaaccacgct tcgagaatgc ggacccgcac ccgcgggcgc cagagctgcc cgctggtgct
    12961 gcgcgcgccg ttcggtggcg gcatccacgc gccggaacat cactccgaga gcgccgaggc
    13021 catgctggct cacattccgg ggctgcgggt ggtcattccc tcgtcgccgc ggaccgccta
    13081 cgggctgctt ctggcagcca tccgggaccc ggacccggtg gtcttcctgg agccgaagcg
    13141 cctgtaccgg tcggccaaag aggctttcga agacgacggc gcagccctcc cgctcgaccg
    13201 ttgccacatc gtccgtcccg gaaaggatct caccctcgtc acctggggag ccatggtcca
    13261 ggaaaccctg gccgccgccg agcagctgtc cggcgaaggc gtcgccgcag aggtcatcga
    13321 cgtcgccacg atcaaacccc tggacggcga gacgatctgc gaatcggtgg ggcgaacggg
    13381 ccgtctcgtc atcgtccacg aagccccgat cacgggcggt ttcggcggag aaattgccgc
    13441 ctgcgtggcc gaacgcgcgc tgtacagcct gcaggcaccc atcctgaggg tggccggcta
    13501 cgacaccgtg atgccgctgg ccaggctcga acatcattac atgccgagcg tgtcgagaat
    13561 cgtcggcgcc gctcgggaaa cgctggaggc ttcatgatcg ctactttcga caacgtcgac
    13621 gagctcgggc ccgagaaggt catcgagatt tacgacccga gcacgggcct gaaggccatc
    13681 gtggtggtgg acaacgtcgc cgccgggccg gccatcggcg gggtcaggat ggccgtcgac
    13741 gccacgcgcg aggagtgtgg gcggcttgcc agggcaatga cgctgaagaa cgccatggcg
    13801 gggcttgctc acggcggcgc caagtctgtg atcgtcgccg acccggccat ggccgacgaa
    13861 gcaaaggaag tcctgattcg aagcttcgcc cacgccatcg ccgacctgcg agactacata
    13921 cccggtcccg acatgggtac ggacgagacc tccatggcac tggtcaggga tgtcaccggc
    13981 cggtccgccg gcctgccgcg ggagctgggg ggcattccct tggacgagat cggcgcgacg
    14041 ggtcttggct tgagcgtttc cctggaggtc gccgaggcct ttacggatct cagcctcgat
    14101 ggcgccagga tcgtggtcca gggattcggc tccgtgggcc agcacgcggc ccgctttctc
    14161 gcccgacgcg gcgcccggct ggtgggcgtc tccgacagcc ggggagcgcg ggcggccgca
    14221 gagggtttcg atctcgatct cctgctcgca tccaagcagg agggcgtcag cgtcggcgcc
    14281 ttcgacgaag gcgaggccat cgatcccgag gatctcgtcg agctcgattg cgacgtgtgg
    14341 attccggccg cccggccgga cgtgctcaac ggggcaaacg ccggccggat ccgagccaag
    14401 gtagttgccc agggagcgaa catccccgcc accccggagg cggagcagac cctggcggcg
    14461 cgcggcgttc tggtgatccc ggacttcgtc gccaacgccg gcggggtcat ctgcgcggct
    14521 gtggagtatc acggcggcag tgaaagccag gccctggcca caatccagga caagattcac
    14581 ggcaacgtca aagaggtgct ctccagggcg accaccacgg gcgagctgcc gagaaaagtc
    14641 gccatggaca ttgctcgcga acgggtgcaa gcggccatgc gctatcgtaa aacctactga
    14701 cccggagtga tgccccggac ggcagcgcct cgccccggcg aagacccagc gcgcccctgg
    14761 ctcagggaat acggcccacc agctcccggc cgacccgctc gagcacgtcg aaccgggtat
    14821 cgaaattggg caggatcatc acctgatcca gacccgcttc gctgagggcg gcaagccggt
    14881 cgatgagctg gtcgcgagtg cctatcatgc aggtggtctc gagcacctcg cgggtcagaa
    14941 accgctcctc ttccggcaac acccagcagt tgtggccggc atggatccgc tggtggcgcc
    15001 tttcctgcgg ataagattcg agcagagcgc agtagtcgtc ccatatgccg ccaagatagc
    15061 cgggcggctg gtgaccgaaa ttgcgccact gatcgtaggc gtagtgaacg gaggccatgg
    15121 ccatggcgcc gcattcggct cgcacccggg gcgaatcgct cgcttccccc gggtccagca
    15181 cgacgatggc ggtcagtccg gtcgtgtaga agtctgatcg atccagcgtc cggcccgctg
    15241 ccgccgcgcc ctcttccagc atgcgccaga tgttggccat caccgccggg ttcgccggca
    15301 tggccagcac cgcgccgtcg ccgtgcttgc ccgccagacc cagagaccgc ggtccgaagc
    15361 cggaaacgta gacggggatt cgatcgtcga aattgacgaa ccccttgtcc ggcatgatgt
    15421 gccgaatggg cgcctcgccg cgggcgccga cggtcaaagg tcccatctgc gcctcctcgc
    15481 cacggaccag cggcacgagg gtgctcagat accggtcgaa ctcggcaatt cgatgaggcg
    15541 gctgccccat gacgcgcatt gccgtgttgc cggcgccgac cccgaggaac gtccgcccgg
    15601 gcgcgatggc gttgatggtc gcaatggccg ccgcgttgac gggtgccggc cgggtgcccg
    15661 agacggccac gccggtgccg aggcggatct tctgggtccg gtccgcggcc agcgccagag
    15721 tggcgtaaca gtcggaccag agcatctggc tgtcggcgag ccaggcgtgg gaatatccga
    15781 gggcctcggc gcgaacgacg tagtcgagat cgccgatgtg agaggcgacg cagattccga
    15841 tgtccatcct gatctccggg atagtgaacc tggccgatta gcgtacttaa gggcgccagc
    15901 gcttgccaga cgatcctggc cgggacgccc cagccgtccc cttgcatccg aacggctacc
    15961 tgcgctaaat ggatcggcat atccagccgg gaaatcagcc atggggcagc tcaagttcga
    16021 ccacctgcag cccatcgtcg acgacgacgc gaccatcgag gccgccctcg ccgatgccca
    16081 catcccgtcg ctgatggccg ccatggtgca cgtcagcggt gatcccagct ggatccgcgg
    16141 agccattcgc ccggtcaacc agttcttcgg cgactcccag ggaggcatcg cgcccaatga
    16201 tcaggcagag gtccgcagac gggcgctcga ggcgctgatt gcctatcgcg atggcaaagc
    16261 gacgcccccg cctcccgacc ccgcaaccgt cagcgagatg gtgaacttcg tcatcggcca
    16321 ggaagtcacc tcggactac
//
LOCUS       DBNHLM010000156         4972 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1040166, whole genome shotgun sequence.
ACCESSION   DBNHLM010000156 DBNHLM010000000
VERSION     DBNHLM010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1040166"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="AC16QF_07225"
     CDS             <1..299
                     /locus_tag="AC16QF_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6448384.1"
                     /translation="YYMNHAENSAYAAEAVNDGYLDMPVLFLGARYDYVCECYDSTLA
                     DPMRERCRRLTETTVYSGHWMAQERPLDVNAALVRWLATDLPEVWPRSRAPDPA"
     gene            312..1889
                     /locus_tag="AC16QF_07230"
     CDS             312..1889
                     /locus_tag="AC16QF_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR6448385.1"
                     /translation="MTTQAGGAGVLARGGLGAVACGHPATACAAAEILEEGGNAFDAV
                     IAAQFAACVAEPVLASVGGGGFLLAHPAPGAPVVYDFFVQTPRSPREASAVDFRPVHA
                     DFGTATQEFHIGLGAAATPGSLAGMEAVHGDLGSLPMARLVEPAARAAREGVRIERLQ
                     ASIFEIVKPIYAASSEARSIYGGIQEGRLLRQSDLADTLEQVARQGAGRFYRGAFADA
                     VARLAQEQGGHLSMEDLLGYGVVKRMPLSLRHAGATVLTNPPPSSGGILIGFGLQLLQ
                     AMAVADQTFGGPSHLAALAEIMAATNQARVELLAAAGSHAELDPALVERYRREVLGTP
                     RFNRGTTHISVVDAKGNAAAMTLSNGEGCGTMIPGTGVMLNNMLGEEDINPDGFGNWP
                     PDQRLTSMMAPTLVETVAGRRLALGSGGSNRIRTAIVQVISNLLDFGDSVDAAVRRPR
                     LHLEGDLLSIEDGFDRDAVRALEAGPAEIRRWPGQSLFFGGVHVAELAKDLFRAAGDP
                     RRGGVGRVVTARGAPEG"
     gene            complement(1914..3596)
                     /locus_tag="AC16QF_07235"
     CDS             complement(1914..3596)
                     /locus_tag="AC16QF_07235"
                     /inference="COORDINATES: protein motif:HMM:NF021354.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPL-N domain-containing protein"
                     /protein_id="HIR6448386.1"
                     /translation="MISSRRGGYAAFFATIVVALTLGASPADGARGGKKGGGGPVCKI
                     TDQTDVAIYVGGGIGPSSQLWAEALIDFWKTGSRIPGEPGLLNDDGGATWTGDGSLDY
                     VTLTLSEFNACRDDDFAKLELFFMPGGSAYEIQANLGASGKDRLTRFLDSGGSYVGMC
                     AGGYYAARGYYWKGDDGAPTDNCRNQFCRYEVDGTFSYEAATSDFTVHEWGGVSYHAD
                     LLAYGPLAEVLVEGPIEEIAGPWHAQSDPNHPYDSHLVTTDDPAMPWLRAVYWGGATE
                     NYVYTDNAPWGTEHAHFAADASGNDDLPFPTDGSLWTLKSVATGTGGAIMISSAHLEA
                     SLFHTASTFTDGGMTECQQYNNYTYLIRRMQSQLGAPFAVPDYDAFCAPDRAGEVKET
                     AVLFPSGLAYQNAPRIDTSGGGGGDGGGDGGGGTGSTSISFDDGTLGDFQLEGDGLRP
                     WQANATAACNGAYGAMAGVPGGVDMESFLTLTVPAGVSRVTYRYSYPSALDRGDDFHV
                     IVNGAVRKSYETGSGAQCATDSLPVTPGDELRFKCRSGGNGETCAVDEIVFD"
     gene            complement(3709..4440)
                     /locus_tag="AC16QF_07240"
     CDS             complement(3709..4440)
                     /locus_tag="AC16QF_07240"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6448387.1"
                     /translation="MANFMLIHGAWHGGWCWHKLVAELSRRGHRALAPDLPAHGHDAT
                     PRDRVTLDAYADSVIEHLNDFAGPVCLVGHSMGGMVISAVGQRVPERLERLVYLCAFV
                     PADGMSLLSSDPTESGAQAFAGKIRGGEDGASVMIDDAAVAALFYHDCTDEDVAFARA
                     RLVPQPSAPLGAAVALTDAAFGSLAKSYIECTADQAAPIAAQRAMYTRAGIDSVVSLS
                     TSHSPFFSAPTLLADSLEALIGTPA"
     gene            complement(4451..>4972)
                     /locus_tag="AC16QF_07245"
     CDS             complement(4451..>4972)
                     /locus_tag="AC16QF_07245"
                     /inference="COORDINATES: protein motif:HMM:NF018649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIR6448388.1"
                     /translation="RYRDLQNRLGILYWPVSFLGIHMMPTVLVFLGCVPLFLIMGRES
                     GPLNGIDLLALAVGAAAVWIETRADLELHRFRARRGSPAEALTTGVWGWCRHPNYLGE
                     IGLWLSLGLFAWAAGADPVWCFAGFVAMVALFELVSIPMIEKKLLAAKPGYEAYRRST
                     FRLLPFSALKRFS"
BASE COUNT          856 a         1708 c         1626 g          782 t
ORIGIN      
        1 cctactacat gaaccacgcc gaaaactccg cctacgcggc agaagcggtc aacgatggct
       61 acctcgacat gccggttctg tttctcggcg cccgctacga ctacgtctgc gaatgctacg
      121 actccacgct tgccgatccc atgcgcgagc gctgccggcg gcttaccgag accaccgtct
      181 actccgggca ctggatggcc caggagcgtc cgctggacgt caacgcggcg ctggtacgct
      241 ggctggcgac cgacctgccg gaggtgtggc cccgctcgcg ggcccccgac ccggcctagg
      301 tctgcctggc tttgacgact caagccggag gggcgggcgt cctggcgcgg ggtggcctgg
      361 gggccgtcgc ctgcggtcac ccggcaaccg cctgcgcggc cgcggagatc ctcgaggaag
      421 gaggcaacgc cttcgacgcg gtgatcgccg cccagttcgc cgcctgcgtc gccgaaccgg
      481 tgctggcatc cgttggcggc gggggcttcc tgctggcaca ccccgctcca ggcgcgccgg
      541 tggtctacga cttcttcgtc cagacgccgc gctcgcctag agaggcttcg gccgtggact
      601 ttcgtccggt ccacgccgac ttcggcaccg caacccagga attccacatc ggcctgggag
      661 ccgcggcaac gcccggctcc ctggccggga tggaagccgt tcacggcgac ctggggagcc
      721 tgcccatggc tcggctggtc gaaccggccg ctcgtgccgc acgggaaggc gtgcgcatag
      781 agcgcctgca ggcaagcatc ttcgagatcg tcaagcccat ctacgcagcg agcagcgagg
      841 cccggtcgat ctacggcggc atccaagagg ggcgcctcct gcgccagagc gacctggccg
      901 acaccctcga gcaggtcgcg cggcaaggcg cggggcgctt ctaccgcggc gcgttcgcag
      961 acgcggtggc acggctggcg caggagcaag gcgggcacct ttccatggag gatctgctcg
     1021 gctacggggt cgtcaaacgc atgccgttga gcctgcgcca cgccggggcc accgtgctga
     1081 ccaaccctcc gccctcctca ggcggaatcc tgatcggctt tggcctgcag cttctccagg
     1141 ccatggccgt tgccgaccaa accttcgggg gcccgtcgca tcttgccgcc ctggccgaaa
     1201 tcatggcggc aaccaatcag gcccgagtgg aattgctcgc cgcggccggc agccacgcgg
     1261 agctggaccc ggcgctcgtc gagcgttacc ggcgggaggt gctcgggacg ccgcgcttca
     1321 accgcggcac cacgcacatc agcgtcgtgg acgcaaaggg caacgccgcc gccatgaccc
     1381 tttccaacgg tgaggggtgc gggaccatga ttccaggaac cggcgtgatg ctgaacaaca
     1441 tgctgggcga ggaggacatc aacccggacg ggttcggcaa ctggccgcca gaccaacggc
     1501 tcacgtcgat gatggcgccc accctggtgg aaaccgtcgc cgggaggcgc ctggccctgg
     1561 gatcgggagg ctcgaacaga attcgcaccg ccatcgtgca ggtcatcagc aacctgctcg
     1621 acttcgggga ctccgtcgac gccgcggttc gccgaccacg gctgcatctc gagggcgatc
     1681 tgctttccat agaggacggg ttcgaccgcg atgcggttcg ggcgctggag gcaggcccgg
     1741 ccgaaattcg ccggtggccc ggccagagcc tgttcttcgg cggcgtgcat gtggccgagc
     1801 tggccaagga tctcttccgg gcagccggcg atccccgccg cggcggcgtc ggacgggttg
     1861 tgaccgcgcg cggagcgcca gagggttgac cttgctcgcc gggcgccgag gtttcagtcg
     1921 aagacgatct cgtccacggc gcaagtctcg ccgttgccgc cggaacggca tttgaaccgc
     1981 agctcatccc ccggcgtcac cggcaacgaa tccgtcgcgc actgcgcgcc gctgcccgtt
     2041 tcgtaggact ttctgaccgc gccgttgacg atgacgtgga agtcgtcgcc ccggtccaga
     2101 gcgctcgggt agctgtatcg gtaggttacc cggctcacgc ctgcaggaac ggtcagggtc
     2161 aggaacgact ccatgtccac cccgccgggc actccggcca tcgcgccgta cgcgccatta
     2221 caggcagcag tcgcattggc ctgccacggc cgcaacccgt cgccttccag ttgaaagtca
     2281 cccaaggtgc cgtcgtcgaa ggagatcgac gtcgatccgg tgccaccgcc gccatcacca
     2341 cccccatcac cgccgcctcc accagaggtg tcgattcgcg gcgcattctg gtaagccagg
     2401 ccggacggaa acaggacggc cgtctccttc acctcgccgg ccctgtccgg agcgcagaag
     2461 gcgtcatagt cgggcacggc aaatggcgcg cccagctggg actgcattcg ccggatgaga
     2521 tacgtgtagt tgttgtactg ctgacactcg gtcatgcccc cgtcggtaaa ggtgctggcc
     2581 gtgtgaaaca gggaggcctc caggtgcgcg ctggagatca tgatcgcccc gccggtgccg
     2641 gtcgccacgc tcttgagcgt ccacagcgac ccgtccgtgg gaaacggcag atcgtcattg
     2701 ccgctcgcat cggcggcaaa gtgggcgtgc tcggtgcccc acggtgcgtt gtccgtgtag
     2761 acatagttct ccgtcgcgcc tccccagtaa acggcacgaa gccagggcat ggccgggtcg
     2821 tcggtggtca ccaggtggga atcgtaggga tggttggggt cgctctgggc atgccatgga
     2881 ccggcaatct cctcgatggg cccctccacc agcacctcgg ccagcggccc gtaggcgagc
     2941 agatccgcgt gataggaaac cccgccccac tcgtgcacgg tgaagtctga tgttgccgcc
     3001 tcgtaggaaa aggtgccatc cacctcgtag cggcagaact ggttcctgca attgtccgtg
     3061 ggagcgccgt cgtcaccttt ccagtagtac ccgcgcgccg cgtagtagcc gccggcacac
     3121 atgccgacat agctgccgcc gctgtccaga aagcgcgtca gccgatcttt gccggaagcg
     3181 cccaggttcg cctgtatctc gtaggcgctc ccgcccggca tgaagaaaag ctccagcttg
     3241 gcgaagtcgt cgtccctgca ggcgttgaat tccgacagcg tgagggtgac gtagtccagg
     3301 ctgccatcgc ccgtccaggt ggccccgccg tcgtcgttga gaagcccagg ctcaccgggg
     3361 atgcggctcc cggttttcca gaagtcgatc agcgcctcgg cccacagctg gctgctgggc
     3421 cctatgccgc cgccaacgta aatcgcaacg tccgtctggt cggtgatctt gcagaccggt
     3481 ccaccgccgc ccttcttgcc gcccctcgcc ccatcggcag gcgaggcgcc caacgtgagc
     3541 gcgacgacga tcgttgcgaa aaacgcggca tacccaccac gccgcgagct gatcatgacc
     3601 cgttgcatat gtattccttc ccgtccttca cgccagtctg tgacccgtgc atgctcgacg
     3661 caacgacgac ttgttaccga ttcgtaagcc aattgcactc ccgaggcctc atgccggcgt
     3721 cccgatgagc gcctcgaggg agtcagcgag cagggtgggg gccgagaaga agggcgagtg
     3781 ggacgtgctt aggctgacca ccgagtcgat cccggcgcgg gtatacatgg ccctctgggc
     3841 agcgataggc gcggcctggt ccgccgtgca ctcgatgtag ctcttggcca ggctgccgaa
     3901 agccgcgtca gtcagggcga cggccgcacc cagcggagcg ctgggctgag gaaccaggcg
     3961 ggctctcgcg aaagccacgt cctcgtcggt gcaatcgtga tagaacaatg cggccacggc
     4021 ggcgtcatcg atcatcaccg aggcaccgtc ttcacccccg cggatctttc cggcaaaagc
     4081 ctgggctccg ctctccgtgg ggtccgagga cagcagagac atcccgtcgg caggcacgaa
     4141 cgcgcacagg tagacgagcc gctcgagccg ctcgggaacc cgctgaccga ccgccgagat
     4201 caccatgccc cccatggagt ggccgaccag gcagacgggg cctgcaaagt cattgagatg
     4261 ctcgataacg ctgtcggcat aagcgtccag cgtcacccgg tcgcggggcg tggcatcgtg
     4321 cccatgagcc ggcagatcgg gcgcgagggc ccggtgcccc cggcggctga gctcggcgac
     4381 caacttgtgc cagcaccagc ctccgtgcca ggcaccgtga atcagcatga agttcgccat
     4441 gatcggccct ctaggagaaa cgtttgagcg cggaaaaagg cagcaggcgg aacgtggacc
     4501 gccgatacgc ctcgtaaccg ggtttggcgg ccagcagctt cttctcgatc atcgggatgc
     4561 tcacgagctc gaagagcgcc accatggcga cgaagccggc aaagcaccag accggatccg
     4621 ccccggctgc ccaggcgaac agcccgagcg agagccacag gccgatctcg ccgagatagt
     4681 tgggatggcg gcaccaaccc cagaccccgg ttgtgagggc ctcggcaggt gagccgcgac
     4741 gggcccgaaa gcgatgcagc tcgagatcgg cgcgggtttc gatccagacc gccgcggccc
     4801 ccaccgccag ggccagcagg tcgatgccgt tcaggggccc cgattcccga cccatgatca
     4861 ggaaaagcgg cacgcacccg aggaagacca gcaccgtggg catcatgtgt atcccaagga
     4921 agctcacggg ccagtagagg attcccagcc tgttctgcag gtcgcgataa cg
//
LOCUS       DBNHLM010000157         6515 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_561498, whole genome shotgun sequence.
ACCESSION   DBNHLM010000157 DBNHLM010000000
VERSION     DBNHLM010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6515)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_561498"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="AC16QF_07250"
     CDS             complement(<1..407)
                     /locus_tag="AC16QF_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448389.1"
                     /translation="MKNKRNQRPLCLVMTLCILLACTGTAVLAAESAEADDAPGFGGP
                     DAVENTLLEEDIRKWKERLQTEHGLGIGMDYSTNYFRASKSLGEKEAWSGMARFYGSW
                     ELANRGGKSSGALVWKVEHRHRISDIPPVALGG"
     gene            complement(445..1656)
                     /locus_tag="AC16QF_07255"
     CDS             complement(445..1656)
                     /locus_tag="AC16QF_07255"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR6448390.1"
                     /translation="MNTALTRSRLTGTLCLVLIAGGCASVPERNPVPPSLADAAEVPG
                     MPGIRYWADDAPPYAKAWLERSQEDLLSRYPASFGNPHNYLAISGGGANGAFGAGLLN
                     GWTDSGTRPEFTVVTGISTGALIAPFAFLGPAYDHVLKRVYTEFSTEDLLESRGLLQA
                     LTGEAATDSAPLRARIAEFVDEEVMDAIADEYQRGRSLLITTTNLDAGRSVTWLIGRI
                     AASGSPNARELIRDVMLASASIPAALPPVVVEVEAGGQTYDELHVDGNTSSVVNLYPL
                     ALDWEAVLQRLEVDAAPNLYIIRNGQIDIAHQSVDRQTIAIAGRSIQTLMNYLARGDL
                     YELYLLTQRDGVRYHLANIPAEFTEAPKEPFDKNYMNALFELGYELAKGGYPWQRVPP
                     NYHESEKARAE"
     gene            complement(1756..3135)
                     /locus_tag="AC16QF_07260"
     CDS             complement(1756..3135)
                     /locus_tag="AC16QF_07260"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase family protein"
                     /protein_id="HIR6448391.1"
                     /translation="MNILGMLLTLILLMTWSSPSRAEDEPARVLFTNVNVFDGYAKDL
                     QRGMHVLVEGNLIKTISDQPIAAADAQVIDGGGRTLLPGFIENHAHLMLMGPSLPAME
                     ANTTWEDFAIHGTRMAEMYLMQGFTTVRDAGGANAGLRRAIDSGQIVGPRFYPSAAFL
                     GTRGGHADFANYTSPVGESTNFGRLNLAQEVDDVADVQKFGRNNFRMGATQLKYMQSG
                     GVVSAFDPWQLLAGSQEEIKAAVQVANEYGSYVMAHSYRKEAIMNALNAGVKSIEHGF
                     AFDCEIAKLMKKKSAYITTNLTAFDPGLLEIPAVASVPSSLAKAKSATATFAGYIDNM
                     KKCPVKRGFQTDCVGSVAACNIQIAYEKHLNNEFFGPHTSLVAMTSVGGEIAALSGDF
                     MNPYPDAKLGIIEEGAYADILIIDGNPFEDFSVVGTGDKWFDAEPRADSPVTIRIIMK
                     DGKLYKNTL"
     gene            complement(3191..3478)
                     /locus_tag="AC16QF_07265"
     CDS             complement(3191..3478)
                     /locus_tag="AC16QF_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448392.1"
                     /translation="MRDEWVIESSYKLQLTRTVTLLPNVRVLIGPAADPGADTTVVLG
                     IRAGIAIWRSPQHPARTQQRTAANSITANVMVAARRRWHEVASGRRIANLG"
     gene            complement(3421..3690)
                     /locus_tag="AC16QF_07270"
     CDS             complement(3421..3690)
                     /locus_tag="AC16QF_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448393.1"
                     /translation="MKGMVTDEIWLGEGVYDANASSGEFDWSSVGEGEYLKHLEVGCT
                     PAFARRAKNTVQLTWWEKDRRKDAGTPCVTNGSSSPATNFSSPVP"
     gene            complement(3687..3959)
                     /locus_tag="AC16QF_07275"
     CDS             complement(3687..3959)
                     /locus_tag="AC16QF_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448394.1"
                     /translation="MSNQIRDADAIRPALFDIEGAIAGYFEWKKQLYDRRGFATSTTL
                     LALYQHADRSISGNKHGAGLIFRWQGSWDLVGRERRRRVSERSALS"
     gene            4137..5075
                     /locus_tag="AC16QF_07280"
     CDS             4137..5075
                     /locus_tag="AC16QF_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR6448395.1"
                     /translation="MAPEPLLAKHGIVADFELIPDYMVSARLLHGYIIEAVSHGPPGA
                     VSVAAGLFNAAFQPNPFSDTAWKSVTLLEAIRRHNANVTRYSPENRFRFSVAKGEGHW
                     EKQDRSPLAETEIFCVANLIGHVRMSLGDGWSPPSVDVSVAEPSTLASLPLFEGVSVR
                     AVPISAAIHIPLSALALPSSSRDAGLSERALPDDFGDLDFVQSMRELIRSYVRVGELS
                     FSNVALAAGLSRRTLQRRLSDVGISYSGMVDQVRFEVARELLLESPELPVTQIGYELG
                     YADPGSFTRAFRRFAGTSPIAFRRASPSGMPTAEVA"
     gene            5191..5865
                     /locus_tag="AC16QF_07285"
     CDS             5191..5865
                     /locus_tag="AC16QF_07285"
                     /inference="COORDINATES: protein motif:HMM:NF019500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR6448396.1"
                     /translation="MRPLTSWRIVQALGKQRYLCLSLLAFLFALPLAGDEPGARLTTQ
                     LVLVFVFITGPLAVARTRATLWITGVFAIVVFVMGIGSVLGGGDWFLGTSALVGFVFF
                     GFLSWLVTRELLFDVKEVTAETLWAAVNVYVLIGLSFAFLYSVLALSSPDAFVGKFMS
                     EPLRDQLLGFVYFSFVTLTTLGYGDITPNNTFVGTLTYLEALVGQLYVAIMIARLVGL
                     YISSQK"
     gene            5992..6492
                     /locus_tag="AC16QF_07290"
     CDS             5992..6492
                     /locus_tag="AC16QF_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024828.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HIR6448397.1"
                     /translation="MNGQSLLAASLIAAVAWSAPAAAQTLASSMGIFIYPTQSQTSDQ
                     QSRDDYECFGFAKTQSGYDPMNPPQITAQAPDQGPSGARVAGAARGAAGGAVIGAIAG
                     DAGKGAAYGATLGAMGGGRADRQRRAQQSQQAEQSAQQQMANMQLTFKTAYGACIEAR
                     GFSVTY"
BASE COUNT         1097 a         2000 c         2082 g         1336 t
ORIGIN      
        1 tctccgccga gagccacggg cgggatgtcg ctgatgcggt gacgatgctc caccttccag
       61 accagcgccc cggagctctt gccaccccgg ttggcgagct cccaggagcc gtagaagcgc
      121 gccatgccgc tccaggcctc tttctcgccc aggctcttgc tggcgcgaaa atagttggtc
      181 gagtagtcca tgccgatccc gaggccgtgc tcggtttgca gccgctcctt ccacttccgg
      241 atgtcctcct cgagcagcgt gttctccacg gcgtcgggtc cgccgaagcc gggagcgtcg
      301 tcagcctccg cggactccgc cgcgagcacg gcggtacccg tacacgcgag caggatgcaa
      361 agggtcatga cgaggcagag cgggcgttgg tttcgcttgt ttttcatgga catctcatcc
      421 gatcgaccga tcgtccggca cgcatcactc ggcgcgggcc ttctcggatt cgtggtagtt
      481 gggcggcacc cgttgccaag gatagccgcc tttggcgagc tcgtagccaa gctcgaagag
      541 cgcgttcatg tagttcttgt cgaagggctc cttgggcgct tcagtgaact ccgccggtat
      601 gttggccaga tgatatcgaa cgccgtcccg ctgggtcagg agatacagct cgtagagatc
      661 gcctcgggca agatagttca tgagggtctg aattgacctt cccgcgatgg cgatggtctg
      721 ccggtcgacg ctttggtggg cgatgtcgat ctggccgttg cggatgatgt agagattggg
      781 cgctgcgtcg acttccagcc gttgcagcac cgcctcccag tccagggcca gcgggtacag
      841 gttgaccacg gagctggtgt tgccgtctac gtggagctcg tcataggtct gacccccggc
      901 ctctacctcg acgactaccg gcgggagtgc tgcaggaatg gacgccgacg cgagcatgac
      961 gtcgcggatg agctctcgcg cgttgggtga gcccgaggcg gcgatccggc caatcagcca
     1021 ggtcaccgat cgtccggcgt cgagattggt ggtggtgatg agcagcgacc tgccccgctg
     1081 gtactcgtcg gcaatggcgt ccatcacctc ttcgtcaacg aactcagcta ttcgggctcg
     1141 cagcggagcg gaatcggtgg ctgcctcacc ggtcagcgct tgcagcaggc cccgggactc
     1201 gaggaggtcc tcggtggaga attcggtgta gacccgcttc aagacgtgat cgtacgccgg
     1261 gccgagaaag gcgaacggcg cgatcagcgc cccggtgctg ataccggtga ccaccgtgaa
     1321 ctccgggcgg gtgcctgagt ccgtccaccc gttgagcaac ccggctccaa acgcgccgtt
     1381 ggctcccccg cccgagatgg ccaggtagtt gtgggggttg ccgaaactcg cggggtaacg
     1441 cgagagcaga tcttcctggg accgctccag ccaagccttc gcgtagggcg gcgcatcatc
     1501 tgcccagtat cggatgccgg gcatcccggg aacctcggca gcgtctgcca gcgagggagg
     1561 aaccgggttg cgttccggta ccgaggcgca cccgccggcg atcagcacaa ggcagagggt
     1621 tccggtcagg cgggacctgg tcagcgcggt gttcatccgg gggctcgcga cctcgagatc
     1681 tccgggcgtg ccacgccgac tggtccggcc cgctggacga gcttcctggt cggcctgaca
     1741 gccctcgtaa gttggctaga gagtgttctt gtagagtttc ccgtccttca tgatgatgcg
     1801 gatggtaact gggctatcgg cccgcggctc cgcatcaaac cacttgtcgc ccgtaccgac
     1861 gaccgagaaa tcctcaaacg ggttgccgtc gataatcagg atgtcggcat aggcaccttc
     1921 ctcgatgatg ccgagtttgg cgtcggggta ggggttcatg aaatcgccgc tcaaagcggc
     1981 gatctcgcca ccgacggacg tcatcgccac caaggaggta tggggtccga agaactcgtt
     2041 gttcaggtgc ttctcgtagg cgatctggat gttgcacgcg gcgactgatc cgacgcaatc
     2101 ggtctggaag ccgcgtttga cgggacactt cttcatgttg tcgatatagc cagcgaacgt
     2161 tgccgtcgct gatttcgcct tggccaagct cgacgggaca ctcgcgaccg ccgggatttc
     2221 gagaagaccg ggatcgaacg ccgtcaggtt ggtggtgatg taggcgcttt tcttcttcat
     2281 cagtttggcg atctcgcagt cgaacgcgaa gccgtgctcg attgatttca cgcctgcatt
     2341 cagcgcgttc atgatcgctt ccttcctgta ggaatgcgcc atcacgtagc taccgtattc
     2401 attggcgacc tgcacggctg ccttgatctc ttcctgtgat ccggcaagca actgccaggg
     2461 gtcgaaggcc gaaaccacgc caccggactg catgtacttc aactgcgtgg cgcccatccg
     2521 gaaattgttg cggccgaact tctggacgtc cgcaacgtcg tcgacctcct gcgccagatt
     2581 caggcgaccg aagttggtgg attcgccgac cggtgaggtg tagttcgcga aatccgcatg
     2641 accgccccgg gtaccgagaa acgctgcgga ggggtagaag cgcggcccga cgatctggcc
     2701 tgaatcgatc gcacggcgca ggccggcgtt cgcgccaccg gcatcacgca ccgtggtgaa
     2761 gccctgcatc aggtacattt cggccatgcg cgtaccgtgg atggcaaagt cctcccacgt
     2821 cgtattggct tccatagccg gcaggctcgg acccatcaac atcaggtggg catggttttc
     2881 gatgaaaccc ggtagcaggg tccgaccgcc gccatcgatc acctgggcgt ccgcggcggc
     2941 gataggttgg tccgagatcg ttttgattag gttgccctct acgagcacgt gcattccgcg
     3001 ctgcaggtcc tttgcataac cgtcaaaaac gttcacgttg gtgaagagaa cgcgtgccgg
     3061 ctcatcctcg gcccgggatg gcgacgacca ggtcatcagc aaaatgaggg ttagaagcat
     3121 gcccagaata ttcacgttgt cctccttggg tggtgccatg ttttggagat ggagtttgca
     3181 atgcaaacgg tcatcctagg ttggcgatcc ggcgcccgct tgccacttcg tgccaccgtc
     3241 gacgcgcagc gaccatgacg tttgccgtga tggagttggc ggctgtgcgt tgctgtgttc
     3301 gggctggatg ctgtgggctg cgccagatgg ctatgcctgc ccttatcccg agcaccacgg
     3361 tcgtgtctgc gccggggtcg gctgcggggc cgatcaggac ccgcacgttg ggtagcagcg
     3421 tcacggtacg ggtgagctga agtttgtagc tggactcgat gacccattcg tcacgcacgg
     3481 cgtcccggcg tctttccgtc gatccttttc ccaccacgtg agctggactg tgttcttggc
     3541 tcgacgggcg aaggccggtg tgcagccgac ctcgaggtgc ttgaggtact cgccctcacc
     3601 gacggagctc cagtcgaact cgccgctgga cgcgttggcg tcgtaaaccc cttcgcccag
     3661 ccagatctca tcggtgacca tgcccttcat gacagcgccg atcgctccga cacccgtcgt
     3721 ctgcgttccc ggccgaccag gtcccagctt ccctgccagc ggaatatcag gccggcccca
     3781 tgcttgttgc cggagatgga cctgtctgca tgctgataca gggccaaaag agtggtgctc
     3841 gtggcgaatc cccgacggtc gtagagctgc tttttccatt cgaaatagcc cgcgatcgcg
     3901 ccttcgatgt cgaacagggc cgggcgaatt gcatcagcgt cgcgaatctg gttgctcacc
     3961 gattcaaggc cgccgtcggt agacgatcct ggcggccccc acttgccatt tggtgccact
     4021 ttcgcgatgg taccgggcgg cgcgagcggt actcaaggtc ctaccaagtg caaccaatct
     4081 ggctactcag gaacacgtat ctcatcgctt acatgcatgc gttacgggag catggcgtgg
     4141 cgccggagcc cctgttggcg aaacacggga tcgtggcgga tttcgagctg attcccgact
     4201 acatggtctc ggcgcgcctg ctgcacgggt acatcatcga agcggtgagt catggaccgc
     4261 ccggtgccgt gagcgtcgcc gcagggttgt tcaacgctgc ctttcagccc aaccccttct
     4321 ctgacacggc ctggaaatcg gtcaccctgc ttgaggccat tcgcaggcac aacgccaacg
     4381 tgacgcgcta cagcccggag aaccgctttc ggttttcggt cgcaaagggc gagggccatt
     4441 gggaaaagca ggacagatcg ccgcttgcgg agaccgagat tttctgtgtc gccaacctca
     4501 tcggtcacgt acgaatgagc ttgggggacg ggtggtcgcc cccgtccgtg gacgtatcgg
     4561 tggcggagcc atcgaccctg gcatccttgc cgctgttcga aggcgtgtcc gttcgcgcgg
     4621 tgccgatttc tgcagctatt cacatccccc tttctgcgct ggcattaccc agcagttccc
     4681 gggatgccgg tttgagcgag cgggctctgc cggatgactt tggtgatctg gacttcgttc
     4741 aatccatgcg ggaactgatc aggagttacg tgcgcgtggg cgagctgagt ttcagcaacg
     4801 ttgccttggc tgcaggcttg agtcggcgaa cgctccagcg acgcctctcc gatgtgggca
     4861 tctcctattc cggcatggtc gaccaggtta gattcgaggt tgcgcgcgaa ttgctcctag
     4921 agtcgccgga gctacccgtc actcagattg gctacgagct gggttacgca gatccaggat
     4981 cgtttacccg cgcatttcgt cgtttcgccg gcacctcgcc catagcattt cggcgcgctt
     5041 ctccgtcggg catgccgacc gcggaggtgg cttgaggcgc acggggcaag ctcgagacgc
     5101 tggcgtgatt gctggcgtga ttgctggcgt gattgaagga atcttgagct tccctttgag
     5161 cggagcgggc tggctcggac gatgaccgag atgcggccac ttacgtcctg gcgaatcgtt
     5221 caggcccttg gcaagcagag atacctctgc ctgtcgctgc tcgcgttcct gttcgcgctg
     5281 ccactcgccg gggacgaacc cggcgcccgc ctgactacgc agctcgtgct ggtgttcgtg
     5341 ttcattaccg ggccgctggc cgtcgcccgc acccgcgcca ccctctggat cacgggcgtg
     5401 ttcgccatcg tcgtgtttgt catggggatt ggttccgtcc tgggcggagg cgattggttc
     5461 ctgggcacgt ccgcactggt cggcttcgtc ttctttgggt tcctgtcctg gctggtgacc
     5521 cgcgaactgc tgttcgacgt caaagaggtg acggcggaga ccttgtgggc ggcagtgaac
     5581 gtctacgttc tgatcgggct gagcttcgcc ttcctctact cggtgctggc gctctcgagc
     5641 cccgatgcgt ttgtggggaa gttcatgtcc gagccgctgc gggaccagtt gctggggttc
     5701 gtctacttca gcttcgtgac cctcacgacc ctcgggtacg gggatatcac gccgaacaat
     5761 acctttgtcg gcaccctgac ttacctggag gcgttggtcg gccagctgta cgtggcgatc
     5821 atgatcgctc ggctggtggg gctgtacatc tcatcgcaga agtagcttcc gggaacgggt
     5881 aggtcggagc ctcatcggaa actcgcgctt gcagtgggcg ttgcagcgat cgtataaagg
     5941 cgggcccacg gggtagggct catgcccact ccaggattca aggaggacta aatgaacggt
     6001 caatccctac tggcggcttc gctcatcgcc gctgtggcct ggtctgcgcc agcagcggcc
     6061 cagacgctcg catcttccat gggcatcttc atctacccga cccagagcca gacgtcggac
     6121 cagcaatccc gggacgatta cgaatgcttc ggctttgcca agacccagag cggttacgac
     6181 cccatgaatc ctccacagat cacggcgcag gcgccggatc agggcccgtc cggggcccgg
     6241 gttgccgggg cagcgcgggg tgctgccggc ggcgccgtca tcggtgccat cgccggcgat
     6301 gcgggcaagg gcgcagccta cggcgccacg ctcggagcca tgggcggcgg ccgagccgac
     6361 cggcagcggc gggcccagca atcccagcag gcggaacagt ccgcgcagca gcagatggcc
     6421 aacatgcagc tcaccttcaa gaccgcctac ggcgcctgca tcgaggcccg gggcttctcc
     6481 gtcacgtatt aagggaaagg agaagaacga atgcg
//
LOCUS       DBNHLM010000158         7483 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_740820, whole genome shotgun sequence.
ACCESSION   DBNHLM010000158 DBNHLM010000000
VERSION     DBNHLM010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_740820"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2041)
                     /locus_tag="AC16QF_07295"
     CDS             complement(<1..2041)
                     /locus_tag="AC16QF_07295"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIR6448398.1"
                     /translation="MTRIMWLGIAGLCAAVLSLAGAFLYLDPQIPRAETYRQVKLETP
                     LRVYSSDEKLIAEFGERRVIPLKLEEAPPLFVAALLDTEDKRFYDHSGIDYISLLNDT
                     VELALNREIRSGASTITMQLARNISFSLEQTFIRKFKEMLLALQIEQELGKDEILELY
                     MNVVPFGKRAYGAQAAALTYYGKRLDELNLAQLAMLAGIPQAPTAGNPINGPARAVRR
                     RNVVLRRMLDQGSISQAAFDEAIAAPITASLHDRALDVGAPFPAEWVRAELINRYSDL
                     YTGGYQVYTTIVADLQQTATRSLRAGLKSYDRRHGYRGPEQSLGALTEEEYLQALREA
                     RSYADLDPAVIVSVDEQGADALLGDGSRGRIAMAEMSWARRYLDVDRRGPPPERPSDV
                     VQVGDVVRVEAIAEPAEAGEAESAGDDPEAEPAPAWRLAQLPDIQGALVSMDPRTGAV
                     LALEGGLDFAMNQYNHALQAARQPGSGFKPFVYSAALDRGVTPASIFMDAPLVFDDQN
                     LESQYRPDNDNNRYNGPTRLREALYRSINLVSMRVLLEVGAGSVMDHVRKFGFDTSTF
                     PRNLQLAIGGGTMAVTPLQMATAYAVFANGGWQIEPHIVDRVVALDGETVYQASHPRV
                     CDPCDQDPATDLDSEEPANLEDLLAEADDAPIKAERVVDERNTYIMNDMLKDVIRV"
     gene            2175..3239
                     /locus_tag="AC16QF_07300"
     CDS             2175..3239
                     /locus_tag="AC16QF_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HIR6448399.1"
                     /translation="MFGLFKNKSNVLLGLDISSTSVKLLELSKSNDRFRVESYAVEPL
                     PLNAVVEKNISDVEGVGEAIKKVVSRSKSSCRRAAVSVAGSAVITKTIEMDASLSDED
                     MENQITVEADQYIPYPLDEVALDFEVQGLSERNPDQVEVLLAACRKENVELREAALDV
                     GQIKPGVVDVEAHAMQRAFDLVKPRLGGNPEELVVAIIDIGATMTTLSVLVDGKSVYT
                     REQLFGGKQLTEEIQRRYSLSFDEAGLAKKQGGLPDDYQSEVLEPFKEAVLQQVTRSL
                     QFFFSSSQFDDVDYIVLAGGTASIDGLAEMVEGKLGTPTIIANPFADMAFAGRIDKDQ
                     LMNDAPALMIACGLAMRSFD"
     gene            3239..3811
                     /locus_tag="AC16QF_07305"
     CDS             3239..3811
                     /locus_tag="AC16QF_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017641026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HIR6448400.1"
                     /translation="MARINLLPWRDWERERRQKEFMANLGGVLIIAVVIVFAAGFMLD
                     RSVEHQDERNRFLQTHLDELDRQIAEIRDLKKKKDELLSRMRVIQELQGNRPVIVRVF
                     DELVRTLSKGVHYRKLSMNADQLVVQGTAESNNRISSLMRNLDESDWFAGPNLKSIKE
                     DPTNIDYGEQASSFDLTFVQVNPNQRDQEQ"
     gene            3813..4424
                     /locus_tag="AC16QF_07310"
     CDS             3813..4424
                     /locus_tag="AC16QF_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017278462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus inner membrane component PilO"
                     /protein_id="HIR6448401.1"
                     /translation="MAWQDTLEQLQSVDLGELDFNNMGSWPGAVKGIVMTLLFAAVVT
                     GGYFYYLTDKQELLERSQTKEIGLRGEYESKAAQAANLEAYREQKQEMEATFGALLRQ
                     LPSDTEVPGLLEDITLTALDNELTIESIDLRAENRTEFYVELPIDIVVEGDYHKIGSF
                     VSGVANLSRIVTLHDFAVTPQGSNQDLKMTILAKTYRYLDEEG"
     gene            4424..4957
                     /locus_tag="AC16QF_07315"
     CDS             4424..4957
                     /locus_tag="AC16QF_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HIR6448402.1"
                     /translation="MVKLTKSTVVVLLSSALSGCWGGDDFKDLREFMADVHARPAPPI
                     EPLPAFEQVPPFAYQASNQRSPFEPPVVIKRVERQEGLPQVVPDFNRVKQFLEQYTIA
                     RLSMVGTLAKGSSMFALIKDGDGGVHRVKAGDYMGTDHGQIVSIDEGAIELIEIVPDG
                     TGGWVERARTVSLGGGA"
     gene            5153..7117
                     /locus_tag="AC16QF_07320"
     CDS             5153..7117
                     /locus_tag="AC16QF_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HIR6448403.1"
                     /translation="MTFDGTPPEPTGYTIEQPARIVLDMPGVESALEDKHHNLGIGNA
                     RRMSVVSTKDRTRAIVNLTQLVSYETEIQGNTLYLTVGSGGVSGVPAPQEYSAVGDVS
                     DRGERDFDDSQINNVDFRRGEDGSGRVIIELSNPKAPVNVVSDGGRIRVEVRNTELPK
                     DLQRRLDVTDFATPVQVVDALQEGPHTTFTVTATGNYDYLAYQADNTLSINVNPLTEE
                     EIETREDIFRYTGEKLSLNFQDIEVRSVLQLIADFTDLNLVASDTVSGRITLRLKNVP
                     WDQALELILKTKGLDKRQVGNVLLVAPAAEIAAREKLELENQKQISELAPLRTEFIQI
                     RYASATELFELFNNAASENRESMLSERGSVIVDERTNAIILTDTAEKLEDFRRVIAQL
                     DVPVRQVLIEARIVIATTSFSEELGIRWGGAALKNNGASKLKYGGSLDTLTELQDTVN
                     GGAFAPSHPNDLIVDLGVTAANATSFGVGITGEDYLLDLELSALATDGHGEVVARPKV
                     ITADKSTAVIESGTEIPYQEASSSGATSTSFKDAVLSLEVTPQITPDDRVIMELNVKQ
                     DSVGDIFNGVPSIDTNEIETQVLVDNGQTVVLGGIFSDTKNVSTTKTPFLGDLPYVGR
                     LFRKELENEEKTELLIFITPKILQDTVSAR"
     gene            7179..>7483
                     /locus_tag="AC16QF_07325"
     CDS             7179..>7483
                     /locus_tag="AC16QF_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010955625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR6448404.1"
                     /translation="MRTQNLFLVGLMAVGKSTVGRCLARDLDMAFYDTDEVLEERAGA
                     EVAWIFDVEGESGFRDREQQVIEELTAKSGVVLATGGGAVLRAANRQNLSARGIVVHL
                     "
BASE COUNT         1491 a         2217 c         2370 g         1405 t
ORIGIN      
        1 cgacccggat cacgtccttg agcatgtcgt tcatgatgta ggtattccgc tcgtcgacta
       61 cccgctcggc cttaattggc gcatcgtcgg cctcggcgag aagatcctcg aggttcgcgg
      121 gctcctcgct gtcgaggtcc gtagcgggat cctgatcgca gggatcgcaa accctcgggt
      181 ggctggcctg gtagacggtt tcaccatcca gcgcgaccac ccgatcgacg atgtgcggct
      241 cgatctgcca gccaccgttg gcaaagacag cataggccgt ggccatctgc agcggcgtga
      301 ccgccatggt tccgccgccg atggcaagct gaaggttgcg cggaaacgtg ctcgtgtcga
      361 agccgaattt gcgcacgtga tccatgacgc tgccggcgcc gacctcgagc agcacgcgca
      421 ttgagaccag gttgatggat cggtagaggg cttcccggag cctggtcggg ccgttgtagc
      481 gattgttgtc gttgtcgggc cggtactgcg actccagatt ctgatcgtcg aacactaagg
      541 gcgcgtccat gaatatgctc gccggcgtga ccccgcggtc gagcgcggcc gagtagacga
      601 agggcttgaa tccggagccc ggctgccggg cagcctgaag cgcgtgattg tactgattca
      661 tcgcgaagtc caggccgcct tccagagcta aaacagcgcc ggtacggggg tccatggaca
      721 ccagggcgcc ctgaatgtca ggcagctggg ccagccgcca agccggcgcg ggttcggctt
      781 cgggatcgtc tccggcactt tccgcctctc cagcctcggc cggttcggca atagcttcca
      841 cccgcaccac gtcgcccacc tggaccacgt ccgagggccg ctcgggcggg gggccgcggc
      901 gatccacgtc caggtagcgt cgcgcccatg acatctcggc catggcgatg cggccgcggc
      961 tgccgtcgcc gagcagggcg tccgcgccct gctcgtccac ggagacgatg acggccggat
     1021 cgaggtcggc gtaggatctc gcctcgcgca gcgcttggag gtattcctct tcggtgagcg
     1081 cgcccagtga ctgctcaggg cctcggtagc cgtggcgcct gtcgtagctc ttcaggccag
     1141 cgcgcaggga acgggtggcc gtctgctgca gatcggccac gatggtggtg tagacctggt
     1201 acccgcccgt atataggtcg ctgtagcgat tgatcagctc cgcgcggacc cactccgccg
     1261 ggaacggcgc gcccacgtcc agggcgcgat cgtggaggct ggccgtgatc ggggcggcga
     1321 ttgcttcgtc gaaagctgcc tgacttatcg agccctgatc gagcatacgc cgcagcacca
     1381 cgttgcgacg gcgcaccgcg cgcgccggcc cgttgatggg attacccgcc gttggggcct
     1441 ggggaatgcc tgccagcatg gcgagctggg cgagattcag ctcgtccaga cgcttgccgt
     1501 agtaggtcag ggcggccgcc tgcgcgccgt aggcccgctt gccgaacggc accacgttca
     1561 tatacagctc gaggatctcg tccttgccga gctcctgctc gatctgcagc gcgagcagca
     1621 tctctttgaa tttgcggatg aacgtctgct ccagggagaa ggagatgttg cgcgcgagct
     1681 gcatggtgat ggtgcttgcg cccgatcgga tctcccggtt cagcgcgagc tccacggtgt
     1741 cgttgagcag cgagatgtag tcgatgccgc tgtggtcgta aaaacgcttg tcttcggtgt
     1801 ccagaagggc ggcgacgaac agcggcggcg cttcctccag cttcaggggt atgacccgcc
     1861 gctcgccgaa ttccgcaatt agcttttcgt cgctcgagta gacgcgcagc ggagtctcga
     1921 gcttgacctg ccgataggtc tccgcgcggg gtatctgcgg atccaaatag agaaatgcgc
     1981 ctgcaagact gagcactgcg gcacataaac cggcgatgcc gagccacata atacgggtca
     2041 tgacgcgtcg ggaccgcggt ctcatgatgg cggcatagtt acagggaagc gattgctctc
     2101 gtagcaatcc tggtattgat caaaacattg catgacaagg acgtaagtgc ccgcgctgct
     2161 aaggaaaaat taccgtgttt ggcctcttta agaacaagtc caacgttctg cttgggctgg
     2221 atatcagctc cacttccgtc aagttgctag agctgtctaa atcaaacgac cggtttcgcg
     2281 tggaatccta cgcggtagaa cccctgccgc tcaatgcggt ggtcgaaaag aatattagcg
     2341 acgtcgaagg tgtaggtgaa gcgatcaaga aggtcgtgag ccgctccaag agcagctgcc
     2401 gccgtgcggc cgtctctgtc gccggatcgg ccgtcatcac caagaccatc gagatggacg
     2461 cctctctgtc cgacgaggac atggagaacc agatcacggt ggaagcggat cagtacattc
     2521 cgtaccccct ggacgaagtt gctctcgact tcgaggtgca aggcctctcg gagcgcaacc
     2581 cggaccaggt ggaagtgctg ctcgccgctt gccgcaagga aaacgtcgag ttgcgcgaag
     2641 ccgcgctgga cgttggtcag atcaagcccg gcgtcgtaga cgtcgaggcg cacgcgatgc
     2701 agcgtgcctt cgatctggtc aagccgcgtc tcggcggaaa ccccgaggag ctggtggtcg
     2761 ccatcatcga catcggcgcc accatgacta cgctctcggt gctcgtagac ggcaaatcgg
     2821 tctacacacg cgagcagctc ttcggcggca agcagctgac cgaagaaatt cagaggcgct
     2881 acagcctttc gttcgacgaa gccggtctgg caaagaagca gggcgggctc ccggacgact
     2941 accagagcga ggtgctggaa cccttcaagg aagcggtgtt gcagcaggtt acgcgatcat
     3001 tgcagttctt cttctcgtcc agccagttcg acgacgtgga ctacatcgtc ctcgccggcg
     3061 gcacggcatc gatcgacggg ctggctgaaa tggtcgaagg caagctcggc acgccgacca
     3121 ttattgccaa cccgtttgcg gatatggcct ttgcaggccg catcgacaag gaccagttaa
     3181 tgaacgacgc gccggcattg atgattgcct gcggcctggc gatgaggagc ttcgactgat
     3241 ggcccgtatc aacttactac cttggcgaga ctgggagcgc gaacgccgcc agaaggaatt
     3301 catggcgaac ctgggcgggg tgctgatcat cgcagtggtg atcgtgttcg ctgcggggtt
     3361 catgctcgat cgctccgtgg agcaccagga cgaacgcaac cggttcctgc agacgcatct
     3421 ggacgaactg gaccgacaga tagcggagat cagggatctc aagaagaaaa aggacgagct
     3481 gctctcgcgg atgcgcgtca tccaggaact tcagggcaac cgtcctgtca tcgtccgtgt
     3541 tttcgacgag ctggtccgca cgctttccaa gggcgtgcac taccgcaagc tctcgatgaa
     3601 tgcagaccag ctcgtggtgc agggcaccgc cgaatccaac aacagaatct cgtcgctcat
     3661 gcgcaatctt gacgagtctg actggttcgc gggtccgaat ctgaagagca tcaaggaaga
     3721 tcccacgaac atcgattacg gcgagcaggc cagctccttc gatctgacgt tcgtgcaggt
     3781 caacccgaac cagcgggacc aggagcaata agatggcttg gcaggacaca ttagagcagc
     3841 ttcagagcgt cgatctgggc gagctcgact tcaacaacat ggggtcatgg cctggggcgg
     3901 tcaagggaat cgtgatgact ctgctgttcg cagcggtggt caccggcggg tacttctact
     3961 acctgacgga caagcaggag cttctcgagc gctcgcagac gaaagagatc ggtctgcgtg
     4021 gcgagtacga aagcaaggcc gctcaggctg ccaatctgga ggcttaccgc gagcagaagc
     4081 aggagatgga agccaccttt ggggctctgc tgcggcagct gccgagcgac accgaggtgc
     4141 ccggcctgct ggaagacata acactcaccg cgctcgataa cgagctcacc atcgagagta
     4201 tcgatctgcg cgctgagaac cggacggagt tctatgtgga gcttcccatc gacatcgtcg
     4261 tcgagggcga ctaccacaag atcggctctt tcgtcagcgg cgttgcgaac ctttcgcgaa
     4321 tcgtcacgct gcacgacttt gccgtcactc cgcagggctc gaatcaggat ctcaagatga
     4381 cgatcctggc aaaaacctac cgatatctgg atgaggaagg gtgatggtta agctgacaaa
     4441 aagcacggtc gtcgtgctgc tttccagcgc gctgagcggt tgttggggcg gtgacgactt
     4501 caaggatctg cgcgagttca tggcggacgt ccacgcaagg cccgcgcctc ccatcgagcc
     4561 cctgccggcg ttcgagcagg tgccgccctt cgcttaccag gccagcaacc agcgcagccc
     4621 gttcgagccg cccgtggtca tcaagcgggt ggagcgccaa gagggcctgc cgcaggtcgt
     4681 acccgacttc aaccgggtca aacagtttct ggagcagtac accattgctc gtttgtcgat
     4741 ggtgggcacg ctggccaagg gcagctccat gttcgcccta atcaaggacg gtgatggcgg
     4801 tgtccatcgc gtcaaggccg gcgattacat gggcacggac catggccaga tcgtaagtat
     4861 cgatgagggc gctatagagc tcatcgagat tgtcccggat gggacgggcg gctgggtaga
     4921 gcgcgcccgg accgtatctt tgggcggtgg agcatagagg atgaaaacga tgcctacttt
     4981 taacagacct aacctctcgt tcagggaaga aggagtggtc cggcgcatag ctcgcggact
     5041 cacgctgctg acctcgtgtc tggtcgcgtt ggcgctcgca gcgccggcgt ggtccgttac
     5101 catggagtcc gttgaattct cctctctgcc tggcgacaaa actgaaatcc ggatgacctt
     5161 cgacggcacg ccgccggagc cgaccggtta cacgatagag cagcctgctc gaatcgtgct
     5221 cgacatgccc ggcgtggaaa gcgcgctcga ggacaagcat cacaacctgg gtattggcaa
     5281 cgcccgccgc atgtccgtgg tatccaccaa ggaccgcacg cgtgccatcg tcaacctcac
     5341 gcagctcgtg agctacgaaa ccgagatcca gggtaacacc ctgtatctga ccgtcgggtc
     5401 cggcggagtg tccggcgtgc ctgccccgca ggaatactcg gcggtgggtg acgtcagcga
     5461 tcggggcgaa agggacttcg acgattccca gatcaacaac gtcgatttcc gacgcggcga
     5521 agacggcagc ggcagggtaa tcatcgagct ttccaatccg aaagcgcccg tcaacgtcgt
     5581 cagcgatggc ggccgcattc gtgtggaggt tcgcaacacc gagctgccga aggatctcca
     5641 gcgtcgcctc gacgttaccg atttcgccac tccggtgcag gtcgtggacg cccttcagga
     5701 aggtccgcac accacgttca cggtgacggc tacgggcaac tacgactacc tcgcttatca
     5761 ggcggacaac acgttgtcga tcaacgtcaa cccgctcact gaagaagaga tcgaaacgcg
     5821 cgaagacatc ttcaggtaca ccggcgagaa gctctcgctg aacttccagg acatcgaggt
     5881 gcgttcggtt ctgcagctga ttgcagactt cacggatctc aacctggtgg ccagcgatac
     5941 ggtatccggt cgcatcacgc tgcggttgaa gaacgtgccc tgggatcagg ctttagagct
     6001 gatcctcaag acgaagggtc tggacaagcg gcaggtgggt aacgtgctgc tggtggctcc
     6061 ggctgctgag attgcggccc gcgagaagct cgagctcgag aaccagaagc agatctcgga
     6121 gcttgctccc ctgcgcacgg agttcataca gatccgctac gcatcggcca ccgagctctt
     6181 cgaactgttc aacaacgcgg ccagcgagaa tcgcgagtcc atgctctccg agcggggcag
     6241 cgtgatcgtc gatgagcgga ccaacgccat catcctgacg gacaccgctg agaagctcga
     6301 agatttccgt cgcgtgatcg cgcagctcga cgtgcccgtc cggcaggtgc tgatcgaagc
     6361 gcgaatcgtc atcgctacca cgagtttctc ggaagagctg ggaattcgct ggggtggcgc
     6421 agcgctgaag aacaatggtg cgtccaagct gaagtacggc ggctctctgg acacgctcac
     6481 cgagctgcag gacacggtca acggcggcgc ctttgcaccg tctcatccga acgatctgat
     6541 cgtcgatctg ggtgtcaccg cggctaacgc caccagcttc ggtgtcggca tcaccggcga
     6601 ggactacctg ctcgatctcg agctctcggc cctggccaca gatggccacg gtgaggtggt
     6661 tgcccggccg aaggtgatca ccgcagacaa gtcgacggcc gtgatcgaat cgggtactga
     6721 gattccgtac caggaggcct cgagcagcgg cgccacctcc acgtccttca aggatgcggt
     6781 tctgtcgctg gaggtgacgc ctcagatcac gcctgacgat cgtgtcatca tggagctgaa
     6841 cgtcaagcag gacagcgtgg gcgacatctt caatggcgtt ccgtccatcg acaccaacga
     6901 gatcgagacc caggtgctgg tggacaacgg gcaaactgtg gttctcggcg gcatcttctc
     6961 ggatacgaag aatgtatcga ctaccaagac acctttcctg ggcgaccttc cctacgtggg
     7021 cagattgttc agaaaagagc tggagaacga ggaaaagacc gagttgctga tcttcatcac
     7081 gccgaagatt ctccaggaca ccgtgtccgc ccggtgatcg tcgcgggccc agcccgaata
     7141 aactaaacta gcggcgcctc ttcaggcgcc gcttttttat gcgcacgcag aatctctttc
     7201 tcgttggttt gatggctgtg ggcaaaagta ccgtggggcg ttgtcttgcg cgggacctcg
     7261 acatggcgtt ctacgacact gacgaggtcc tcgaggagcg cgcgggggcc gaggtcgcct
     7321 ggatcttcga cgtggagggc gagtcggggt ttcgcgatcg ggagcagcag gtcatcgagg
     7381 agctgacggc aaaaagcggc gtcgtgctgg cgacgggtgg tggcgcggtg ctgcgtgctg
     7441 cgaaccggca gaatctgtcg gctcgaggga tcgtcgtgca cct
//
LOCUS       DBNHLM010000159         3538 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_800601, whole genome shotgun sequence.
ACCESSION   DBNHLM010000159 DBNHLM010000000
VERSION     DBNHLM010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_800601"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="AC16QF_07330"
     CDS             complement(<1..1037)
                     /locus_tag="AC16QF_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6448405.1"
                     /translation="MTEGPIWRPDPVRALESNMARFTEEAARVSGRSMTGYADLHAWS
                     IEEPAAFWDLVWHFTDVRASQAPTAILTNADQLPGARWFEGARLNFAENLLRFDDDRP
                     ALIALLETGERREISYAELRRETAQVAAWLKNHIDPGDRVAGWLPNVPEAVTAMLATT
                     SLGGVWSSCSPDFGAEGALDRFGQIEPKVLFACDGYHYNGKPISTVDKARQVAERIPS
                     IRAIVWISLAGEDLVESDGVAYASLLEGDHDLAFAQRAFDDPLYVMYSSGTTGKPKCI
                     VHGIGGTLIQHLKEHQLHTDLRRDDRLFYFTTCGWMMWNWLVSGLATGATLVLYDGSP
                     FHPGPEALLDMA"
     gene            complement(1034..2173)
                     /locus_tag="AC16QF_07335"
     CDS             complement(1034..2173)
                     /locus_tag="AC16QF_07335"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017255227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase family protein"
                     /protein_id="HIR6448406.1"
                     /translation="MTDVRPFRIEIPEKDLDDLGQRLALTRLPEKETPDDWSQGVPLA
                     YMREIVDYWQSDYDWRRAEEEINRFPQFTTELDGLNVHFIHVKSPEPNALPLVITHGW
                     PGSIVEFLKVIGPLADPASHGGDPADAFHVVCPALPGYGFSGKPITPGWGVEKIGSEW
                     ANLMARLGYESYVAQGGDWGSAVTTAIGMQDPDHCRAIHLNMVVAQPDPATMDDLTDL
                     EKRGIAGMQHYQQWDSGYSKEQSTRPQTIGYSLVDSPVGQAAWIMEKFWAWTDCDGHP
                     ENALSRDEMLDNIMLYWLTASGASSARLYWESFGSFSAGDVSVPTGCSIFPKEIFTCS
                     RRWAEQRFKNIVYWNELDRGGHFAAFEQPELFVGELRNCFRQVRG"
     gene            complement(2200..2970)
                     /locus_tag="AC16QF_07340"
     CDS             complement(2200..2970)
                     /locus_tag="AC16QF_07340"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448407.1"
                     /translation="MSLFRLDNKNIAITGASSGFGHHFAGVLSEAGASVVLGARREDK
                     IRDRVDEINAAGGKATAVKLDVRDADSVRGFLEAAEAAYGPLDVVINNAGVEAGAKTY
                     LMIDEDDWDFVMDTNLKGAWLVSKTYTELVLAAGRKQGNIINISSITDTRTIKGQFPY
                     AVSKGALTRMTEVMALEAAKYGIRVNALAPGYILTDVSRLLLESDQAPEFVKGIPMRR
                     FGEFSDLDGPLLLLASDASTYMTGSVVVVDGGHICASL"
     gene            complement(3043..3303)
                     /locus_tag="AC16QF_07345"
     CDS             complement(3043..3303)
                     /locus_tag="AC16QF_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448408.1"
                     /translation="MPDDELKRARDDARCEAAIQTLTDVLWRFEETCEGSYTLYDLVG
                     YLVEDLVREGCCAACIAESVTAAFKDIGADTTEHRRDEGTIH"
BASE COUNT          579 a         1110 c         1193 g          656 t
ORIGIN      
        1 gccatgtcca gcagggcttc gggacccgga tgaaagggcg agccgtcgta gagtaccagc
       61 gtggcaccgg tggcgagccc gctgaccagc cagttccaca tcatccagcc gcaggtggtg
      121 aagtagaaca gccgatcatc ccggcggaga tccgtgtgga gctggtgctc cttgagatgc
      181 tggatgaggg tgccgccgat cccgtggacg atgcacttgg gctttccggt ggttccggac
      241 gagtacatga cgtacagagg atcgtcaaaa gcgcgttggg cgaaagcgag atcgtggtcg
      301 ccttcgagga gcgaggcgta ggctacgccg tcgctttcca ccagatcttc cccggccagg
      361 gagatccaga cgatggcgcg tatggagggg atgcgctcgg caacctggcg cgccttgtcc
      421 acggtggaga taggcttgcc gttgtagtgg taaccgtcgc aggcgaacag caccttcggc
      481 tcgatctgcc cgaaccgatc cagcgcgccc tcggcgccga agtcgggcga gcacgacgac
      541 cagacgccgc ccagggaggt ggtggccagc atggcggtca cggcttccgg cacgttcggc
      601 agccacccgg caacccggtc tcccgggtcg atgtgatttt tgagccacgc ggcgacctgt
      661 gccgtctcgc ggcgcagctc ggcgtaggaa atctcacgcc gctcgccggt ctccaaaagc
      721 gctatcaggg ccgggcgatc atcgtcgaag cgcaggagat tttcggcgaa gttcagcctc
      781 gcgccctcga accatcgggc tcccggcagc tgatccgcat tggtcaggat cgcggttggc
      841 gcttgggaag ctcgcacgtc cgtgaaatgc cagaccagat cccagaaggc ggcgggctct
      901 tcgatcgacc aggcgtgcag gtccgcatag cccgtcatgg agcggccgga tacccgggcg
      961 gcttcctcgg tgaagcgggc catgttcgat tccagcgccc ggaccggatc cgggcgccag
     1021 atgggcccct cggtcacccg cgtacctggc ggaagcagtt tctcagctcg ccgacgaaca
     1081 gctcaggctg ctcgaaggcg gcgaagtgtc cgccccggtc cagctcgttc cagtagacga
     1141 tgttcttgaa gcgctgctcc gcccaccggc gcgagcaggt gaagatctct ttcgggaaga
     1201 tgctgcatcc cgtcggcacg ctcacgtcgc ctgcggagaa gctgccgaag ctctcccagt
     1261 agagccgtgc ggacgatgcg ccgctggccg tgagccagta gagcatgatg ttgtcgagca
     1321 tctcgtcccg gctcagggcg ttctccggat gcccgtcgca atcggtccac gcccagaact
     1381 tctccatgat ccaggcggcc tggcccacag gtgagtcgac caggctgtag ccgatggtct
     1441 ggggacgcgt gctctgctcc ttggagtagc cggagtccca ctgctggtag tgctgcatgc
     1501 ccgctatgcc tcttttctcg aggtcggtca ggtcgtccat ggttgccgga tccggctggg
     1561 cgaccaccat gttgaggtga atagcgcggc agtggtcggg atcctgcatc ccgatagcgg
     1621 tggtgactgc cgagccccag tcgcctccct gggcgacgta gctttcgtag cccaggcggg
     1681 ccattaggtt tgcccactcg ctgccgatct tctccacccc ccagcccggg gtgatgggct
     1741 tgcccgaaaa gccgtagccg gggagcgccg ggcagaccac gtgaaaggcg tctgccggat
     1801 cgccgccgtg actggcggga tcggccaacg ggccgatcac tttcaggaac tcgacgatgg
     1861 agccgggcca gccgtgggtg attaccaggg gcagggcgtt cggctcgggc gatttcacgt
     1921 ggatgaagtg gacgttcagc ccgtcgagct cggtggtgaa ctgcgggaag cgattgatct
     1981 cttcctcggc gcgtcgccag tcgtaatcgg actgccagta gtcgacgatc tcgcgcatgt
     2041 acgccagcgg cactccctgg ctccagtcgt cgggcgtttc cttctcgggc agccgggtca
     2101 gcgccaggcg ctggcccagg tcgtccagat ccttctcggg tatctcgatc ctgaaggggc
     2161 gtacgtcggt catggtctgt ctcctgcctg gtttcagcgt cacagcgacg cgcagatgtg
     2221 gccgccgtcc acgacgacca cggatcccgt catgtaggtc gaagcgtcgg aggccagcag
     2281 caacagggga ccgtccaggt ccgagaactc gccgaaccgg cgcatgggaa tgcccttcac
     2341 gaactcgggc gcctgatcgc tctcgagcag caggcggctc acgtccgtga ggatgtagcc
     2401 gggcgcgagc gcgttgacgc gtatcccgta cttggccgcc tccagggcca tgacctcggt
     2461 catgcgggtg agcgctccct tggagacggc gtacggaaac tgtcccttta tcgtgcgggt
     2521 atcggtaatg gatgagatgt tgatgatgtt gccctgcttg cgaccggccg cgaggacgag
     2581 ctcggtgtag gtcttggaga cgagccacgc acctttgagg ttcgtatcca tgacgaagtc
     2641 ccagtcatcc tcgtcgatca tcagataggt tttcgcgcca gcctcgacgc cggcgttgtt
     2701 gatgaccacg tccaggggcc cgtaggcagc ctcggcggcc tcgaggaagc ctctgacgct
     2761 gtcggcgtct cgcacgtcca gctttaccgc ggttgccttg ccgccggcag cgttgatctc
     2821 gtcgacccgg tcgcgaatct tgtcttcgcg gcgggcgccg agcaccacgc tggccccggc
     2881 ctcggaaagc acgcctgcga agtggtggcc gaatcccgag gatgcgccag tgatggcgat
     2941 gttcttgttg tcgagccgga acagactcac gcgaaaaccc tcctgccctg acgggagcac
     3001 aatcctagcg acaaagaatg tggcaggcca ggcaggcggt ggtcagtgta tggttccctc
     3061 gtcgcgccgg tgctcggtgg tatcggcgcc gatgtccttg aacgccgccg tcacggactc
     3121 cgcgatgcac gcggcgcagc agccctcccg gaccaggtct tccaccaggt agcccacgag
     3181 gtcgtagagg gtgtaggagc cctcgcaggt ttcctcgaac cgccacagca cgtctgtgag
     3241 ggtctggatt gccgcctcgc agcgggcgtc gtcgcgcgcg cgtttgagtt cgtcgtccgg
     3301 catgtcttgg gctccaggca tgatgcggta tcgggcattg taaccggccg gcgcggggct
     3361 tgcgtcgcgc tttgagcggg acgcgtcggt ggtgcgtcgg tagccgcaaa ccactgagtc
     3421 aaaatggtcg gagtcagagg attgaactgg ccgacactcg gctccggccc ggggcctgcg
     3481 tcccgctttg gcgggacgcg tcggtggtgc gtcggtagcc gcaaaccact gagtcaaa
//
LOCUS       DBNHLM010000160         3409 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1520626, whole genome shotgun sequence.
ACCESSION   DBNHLM010000160 DBNHLM010000000
VERSION     DBNHLM010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1520626"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..1234
                     /gene="xerD"
                     /locus_tag="AC16QF_07350"
     CDS             329..1234
                     /gene="xerD"
                     /locus_tag="AC16QF_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIR6448409.1"
                     /translation="MGEVLPKDEAARHTVDEYLDAVWLERGLSENTLAAYRRDLYQLA
                     DMLKRDPLTANDADLLECLALRHRQGYRASSAARWLSSVKGFYRHAAGRGRIGADPSA
                     HLEHPKMGRRLPGSLSEAEVRQLLAAPRLDDPIGLRDKAMLELLYASGLRISELVGLT
                     MSAVNARQGIVRIVGKGGKERLVPMGGEAMRWLERYLHEGRPLIKGSDATAAMFPSRR
                     GSQMTRQNFWHAVKRYAMKAGIHRSISPHTLRHAFATHLINHGADLRAVQMMLGHADL
                     STTQIYTHVAQARLKDLHRTHHPRG"
     gene            1358..2128
                     /locus_tag="AC16QF_07355"
     CDS             1358..2128
                     /locus_tag="AC16QF_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003756 - protein disulfide isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HIR6448410.1"
                     /translation="MKLSFQTLWVGAVLACLAVIGHADEAAEADEVKDVTDAKGGQAL
                     VERLTALRPGIPIEQVRETPLAGIYALELKGGSFFYGTADGKYLFAGDMYELTDTSLV
                     NLAEAGRTAKRQALMAQVTAEEMVVFSPEGTTKASISVFTDVDCGYCQKLHLEVPKLN
                     EMGIEVRYLAYPRAGVGSQSYQKIVSAWCSADRNTAITRLKARQTIPTLTCSNPVASQ
                     LDLGREVGVTGTPAIVLEDGRLLPGYMPAEQLAEALGI"
     gene            2216..>3409
                     /locus_tag="AC16QF_07360"
     CDS             2216..>3409
                     /locus_tag="AC16QF_07360"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR6448411.1"
                     /translation="MALEPLKVGMCGLGTVAQGVLEVLATNRESITRRAGREIRVTRV
                     ASRRAKPDVDLGRAEFSTDVHALARDPDIDVLLELIGGEDTALELVTSALARGTPVVS
                     GNKAIVALHGNELLPLAAQHGAHLGFEAAVAGSIPIVSSLNRSLAANQHEWLAGIING
                     TSNFILSAMTQSGRAFADALAEAQALGYAEADPTFDVEGIDAAHKLTILTALAFDVGF
                     DFHSVYTEGISKLTAEDIGYARQLGYRIKHLGRARRREHGIEARVHPVLVPEQHLLAS
                     IGGAMNAVLVHSNAAGDTLYSGAGAGALPTASAVVADLIDLARGTARLPSPVGDVDLP
                     ILPIAETDSAYYLRIPSVDRPGAFARVATILSEREISIEAAIQREEARVRTRGAESGD
                     GAWVPI"
BASE COUNT          631 a         1041 c         1138 g          599 t
ORIGIN      
        1 gcccggcggc ttaaaaaaat gaagccagct tgccgggttg ggggagttgg ggggaggccc
       61 cggcaagctg gccgtcttcg gaaaccggtg gagagggggc cggcttcctt cagcgtgggt
      121 cacgatcact ccaggggaca gctgggtggg tggatcgcgc ccgtataagc caatgcagct
      181 accgtgccag ctatctggat tccgttcaaa atcgattaaa aatccttttg tttcatgtag
      241 ttgaataact atccgctggt ttttatgtcg gttgactgag gaccaactac gtcacaaagt
      301 gtccgtgatg ggtgcccaag gagacgaaat gggcgaggtt ctgccgaagg atgaggcggc
      361 ccgccatacg gtggacgaat atctcgacgc ggtctggctc gagcggggct tgagcgagaa
      421 cacgctggcc gcctatcgtc gggatctcta ccagcttgcc gacatgctga agcgggatcc
      481 gctgacagcc aacgacgcgg atctgctcga gtgcctggcg ctgcggcatc gtcagggtta
      541 ccgcgccagc tccgccgccc gctggctctc ctccgtgaaa ggcttctacc ggcacgcggc
      601 cgggcgcggg cggatcggcg cggatcccag cgctcacctg gagcatccga agatgggacg
      661 ccggctgccg ggctcgttgt cggaggcgga agttcgtcag ctgctggcag caccgcgcct
      721 ggacgatccc atcgggctca gggacaaggc catgctggaa cttctctacg cctcggggtt
      781 gaggatatcc gagctggtgg gtctcacgat gagcgccgtg aacgcgcggc aggggatcgt
      841 gagaatcgtc gggaaggggg gcaaggagcg cctggtgccc atgggtgggg aggcaatgcg
      901 ctggctcgag cgttatctcc acgagggccg tccgctcatc aagggcagcg atgccacggc
      961 agcgatgttt ccgagccgcc ggggctcgca aatgacccga cagaacttct ggcacgcggt
     1021 gaagcgctat gccatgaagg cgggcatcca ccggagcatc tcgccgcata cgctgcgcca
     1081 tgcctttgcc acccacctta tcaatcacgg ggcggacctc agggccgtgc agatgatgct
     1141 gggacatgcg gacctgtcca cgacccagat ctacacgcac gtcgcgcagg ctcggctgaa
     1201 ggacctgcat cggacccatc acccgcgagg atgagagcga agcgcgggtt tgagtattgg
     1261 agcaggctat ttgtaaagga atgtggttaa gctggatatt tgggcgcgct cgctggtcta
     1321 atgctccggc cgtgcaggag gcaccgtgaa gaaatcaatg aagttgtcat ttcagaccct
     1381 ctgggtcggg gcagtgctag cgtgcctggc cgtcatcgga cacgccgacg aggcagccga
     1441 ggccgatgag gtcaaggacg tcacggacgc caaaggaggc caggcgctcg tcgagcggct
     1501 gacggcgctg cgtcccggta tacccataga gcaggtacgc gaaaccccgc tcgccggcat
     1561 ttacgccctc gagctcaagg gcggttcgtt tttctacggt accgcggatg gtaagtatct
     1621 gttcgccgga gacatgtacg agctcaccga caccagcctg gtgaacctcg ccgaagcggg
     1681 gcgaacggca aagcggcagg ccctgatggc ccaggtgacg gcggaggaga tggtggtttt
     1741 ctccccggaa gggacaacca aggcgtccat cagcgtgttc acggacgtgg actgtggtta
     1801 ctgtcagaag ctgcacctcg aggtgcccaa gctcaacgag atgggcatcg aggtccggta
     1861 cctggcctat ccgcgggccg gggtgggttc tcagtcctat cagaaaatcg tgtcggcctg
     1921 gtgctcggcc gatcgcaaca cggccattac caggctgaag gcgcgacaga ccattcccac
     1981 gctgacctgc tcaaacccgg ttgcttccca gctcgacctc gggcgcgaag tgggcgtgac
     2041 gggcacaccg gccatcgttc tcgaggacgg gcggctgctg cccggctaca tgccggccga
     2101 gcagctggcg gaagcgctgg gcatctgatc ccctcgggcg aagtttcccg tcgatacctt
     2161 tgagcacagg acggcgcgcc agtagacttg gcgcctcgtc gcagcatctt ggtcattggc
     2221 gttggaaccc ctcaaggtag gaatgtgtgg tctaggcacc gtggcacagg gcgtgctgga
     2281 ggtgctggcc accaataggg aaagcattac ccggcgggcg ggccgggaaa tacgggtgac
     2341 ccgcgtggcc agccggcggg ccaagccgga cgtggacctt ggccgggccg agttttccac
     2401 cgacgtgcat gccctcgcca gggatcccga catcgatgtt ctcctagagc tcattggggg
     2461 tgaggatacc gccctggagc tcgtcaccag tgccctcgcc aggggaacgc cggtggtttc
     2521 gggtaacaaa gccatcgtgg cgctgcacgg aaacgagctc ctgccgttgg cagcccagca
     2581 cggcgcgcac ctcgggttcg aagccgcggt agcgggcagc ataccgatcg tttcaagcct
     2641 caaccggtct ctggccgcca accagcatga gtggctggcc ggcatcatca acggaacctc
     2701 caatttcatt ctcagcgcca tgacccagag tgggcgggcc ttcgccgatg cgctggccga
     2761 ggcgcaggcg ctcggctacg ccgaagccga ccccaccttc gacgtggagg gcatcgacgc
     2821 tgcccacaag ctgacgatcc tcacggcgct cgccttcgat gtcggcttcg actttcactc
     2881 ggtctatacg gagggcatct cgaagctgac agccgaggat atcggctatg cccggcaact
     2941 cggctatcgc atcaagcacc tcggcagagc gcggcgccgc gagcacggca tcgaagctcg
     3001 cgtgcacccc gtgctggtgc cggagcagca tctgctcgcc agtatcggcg gtgcgatgaa
     3061 cgccgtcctg gtgcacagca atgccgcggg cgatacgctc tacagcggtg cgggagccgg
     3121 cgcgctgccg acggcctcag ccgtggtcgc tgacctcatt gatctcgctc gtggcactgc
     3181 cagattgccc agcccggtcg gagacgtcga ccttcccatt ctgccgatcg ccgagaccga
     3241 cagcgcctat tacctgcgga ttccctccgt cgaccgtccg ggcgcgttcg cccgggttgc
     3301 gaccattctc agcgaacggg agatcagcat cgaagcggcc attcagcggg aagaagccag
     3361 agttcggacc cgcggagcgg aatcgggcga tggagcctgg gtgcctatc
//
LOCUS       DBNHLM010000161         9741 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_741092, whole genome shotgun sequence.
ACCESSION   DBNHLM010000161 DBNHLM010000000
VERSION     DBNHLM010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9741)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9741)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9741
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_741092"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="AC16QF_07365"
     CDS             complement(<1..527)
                     /locus_tag="AC16QF_07365"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013112.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0103039 - protein methylthiotransferase
                     activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S12 methylthiotransferase"
                     /protein_id="HIR6448412.1"
                     /translation="MTGKVGFISLGCPKALVDSEQLLTALTAEGYTTADDYSEADVVV
                     VNTCGFIDAAIEESFDAIEEALDACGKVVVTGCLGARKDSLQERYPELLSVSGPQDPV
                     PVVDAVRAHVPLSAAESTHYHLGPAGVKLTPAHYAYLKVAEGCNHTCSFCIIPSMRGK
                     LRSRALPALLEEADRL"
     gene            897..1979
                     /locus_tag="AC16QF_07370"
     CDS             897..1979
                     /locus_tag="AC16QF_07370"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003382491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR6448413.1"
                     /translation="MMSAELENLNVAETEVLVTPAALKALLPVPDAVGESVRASRRAV
                     QNILDHSDHRLLVVVGPCSIHDTDAAREYAQRLKQLADEVSSELFVVMRTYFEKPRTT
                     VGWKGLINDPYLDDSFRVGEGLKIARRLLLDITSLGLPVSTEALDPITPQYLQDLITW
                     SAIGARTTESQTHREMASGLSCPIGFKNGTDGSLDVAINALESAVSPHRFLGISSSGQ
                     VSVIRTTGNRHAHIVLRGGSRGPNYDAESVAACEAALEKIGQVPNIMVDCSHANSNKD
                     HRRQPDVARSLAEQIENGSRSLMGLMIESNLHEGNQPVNGGSNLRYGVSVTDACIDWP
                     TTESLLRELAERLGPALRARAKVAQR"
     gene            complement(1970..2551)
                     /gene="nfuA"
                     /locus_tag="AC16QF_07375"
     CDS             complement(1970..2551)
                     /gene="nfuA"
                     /locus_tag="AC16QF_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HIR6448414.1"
                     /translation="MIEISESAQGYLRDLLANQEEEGVGIRVFVAQPGTPQAETCIAY
                     CRPGEQQEGDVAVAYDGFTAWFEERSAPYLVEAMVDYAEDRMGGQLTIKAPNARLPRV
                     GPDAPLEDRINYVLYNEINPGLAAHGGVVTLVEVLEDMTAILQFGGGCQGCAAVDITL
                     KHGVEKSLLEQIPELTGIKDATDHTMTEHAYYR"
     gene            2726..6403
                     /gene="metH"
                     /locus_tag="AC16QF_07380"
     CDS             2726..6403
                     /gene="metH"
                     /locus_tag="AC16QF_07380"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HIR6448415.1"
                     /translation="MPEDSMPAEPSLIQQLKERILLLDGAYGTMLQALNLDEPDYRGS
                     IFEGHPAPLKGNYDALNLARPELVADVHRAYLGAGADIIKTNSFNSTRIAQGDYALAD
                     RAEQLNAAAARIARRCADEFASPEWPRFVAGVLGPTNRTASLSPDVNDPGFRNVTFEE
                     LEENYYEAAQALLDNGADLLLLETVFDTLNAKAAIFAIRQLGEKRGATVPLIVSGTIT
                     DASGRTLSGQTCEAFWASVNHAEPLAVGLNCALGADQLRGYVARLGRFADTYVSVHPN
                     AGLPNAFGEYDESPESMAAKLGEFARSGLVNLVGGCCGTTPAHIAAIRESVRECQPRA
                     IPERRNDLMLSGLELLTIDDASLFVNVGERTNVTGSARFARLIREEAYDEALVVARQQ
                     VENGAQVIDINMDEGMLDSQAAMVRFLQLLAAEPDIARVPIMIDSSKWEIIEAGLRCV
                     QGKAIVNSISLKEGEDAFEAVARRCRDYGAAVVVMAFDEDGQADTLERKREICKRSYR
                     ILTERIGFPPQDVIFDPNVFAIGTGIDEHRGYAVSFIEACRWITDNLPHARVSGGISN
                     VSFSFRGNNPIREAIHTVFLYHAIRAGLTMGIVNAGQLGIYQEIPDELREKVEDLVLN
                     RRSDATERLLAVADRYGGRASESEGPDLSWREGSVAQRLGHALVKGIGEFIVEDTEEA
                     RLAADHPIEVIEGPLMDGMNVVGDLFGQGKMFLPQVVKSARVMKQAVAHLVPYIEAAQ
                     GPGRKAKGRIVMATVKGDVHDIGKNIVGVVLQCNNYEVIDLGVMVPMEKILDTAQEVG
                     ADVIGLSGLITPSLDEMVQVAGEMQRRGLNLPLMIGGATTSKAHTAVRIEPAYQDGPT
                     VYVSDASRSVSVAARLVSPQQRIGFSEETRKEYEEIRRRTSARSANRKYLSLEAARRN
                     AFVCNWEAYEPPRPASIGVQTIENLPLETLVPYIDWSPFFLTWELAGKYPAILDDPVV
                     GESARDLFRDASQMLEHLCREKRLKANAAFGFWPCNRTGPEELTLFEDEERKTELARL
                     QHLRQQTIRGAGRPNYSLADFVGPPPVADYIGAFAVTAGIGIEDVLADYGDDDYGAIM
                     IKALADRLAEAFAEYLHEQVRQVHWGYAPGESLAAEELIGEAYRGIRPAPGYPACPEH
                     SEKSTLFRLLDAQANAGMTLTESFAMLPAASVAGWYFSHPDARYFGIGKIDDEQVADY
                     ASRKGITALEAEKLLRPNLDR"
     gene            complement(6400..6534)
                     /locus_tag="AC16QF_07385"
     CDS             complement(6400..6534)
                     /locus_tag="AC16QF_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448416.1"
                     /translation="MDDTPTDDARSEADRKADAWAVMIIFWALVAGAVHYVSGWTFDF
                     "
     gene            6721..8382
                     /locus_tag="AC16QF_07390"
     CDS             6721..8382
                     /locus_tag="AC16QF_07390"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011267700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HIR6448417.1"
                     /translation="MYRYDDFDRQFIAERVAHYREQTTRYLAGELSESEFLQLRLRNG
                     LYIQRLAPMLRVAVPYGTLSSGQLRKLAHIARHYDRGYGHLSTRQNMQFNWPELAEVP
                     DILAELAEVDMHAIQTSGSCIRNVTTDQFAGVAADEEVDPRPYCELIRQWSTNHPEFD
                     WLPRKFKIAVTGAASDRAAIAVHDIGLRARREDAEGIVFDVFVGGGLGRTPVIAAEIR
                     KSLPVRHLLTYLEAIMRVYNRYGRRDNKYKARIKILVRAMTAEGFRDRVDAEWAHLKG
                     SESTLTDEEIRRIEAFFTPHEYPPLNDADLGPARLEDPAFAAWLRHNVSPHRVPGYAI
                     VTLSTKVTGVPPGDVSDVQMEAVADWADEYGFGELRLGHEQNFVLPDVPQSRLRELWR
                     RTAALQLGVANVGLISDIVCCPGGDYCSLANAKSIPVAEAIQQRFDDYDYQHDLGELD
                     LNISGCMNACGHHHVGHIGILGVDKKGAEYYQISLGGHSGAGGDEASLGEIIGPSFSR
                     AEVPATIGRILDVYLKRRIDDEPFLHCYRRIGIQPFKDHVYERAA"
     gene            8366..8872
                     /locus_tag="AC16QF_07395"
     CDS             8366..8872
                     /locus_tag="AC16QF_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HIR6448418.1"
                     /translation="MNAQLDYIDQPAAQMVSGPGAVPGPARLVAAEQWTPGSSDGLLL
                     AVDAEPEQRFAGAELIAIDFPAFTDGRGLSLAVLLRTRLGFRGELRAVGDIRADMLHY
                     LYRCGFDSFSMSGEGSPVDADGVTKTTAFDALAPYSDCYQASVRQPRPAYRRVERCIA
                     QELSSPSF"
     gene            8836..>9741
                     /locus_tag="AC16QF_07400"
     CDS             8836..>9741
                     /locus_tag="AC16QF_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR6448419.1"
                     /translation="MHSTGIVESFFLIFTGAAVLATAALYSRQPLLVAYIAVGALLGP
                     HGFKLISDSNLLSEISEIGIIFLLFLVGLDLQPSKLKNMVGESLLTAVGTTIVFFAAG
                     FAMMLAFDFSTSEAVVTGIAVTFSSTIIGIKLLPTTVLHHRHTGEIVVSLLLIQDLIA
                     ILALIVLTGYGDTLGATLSSLGMALIALPLLILGALGGMRLLVLPLIRKFDAFNEFIF
                     LLALGWCLGLASIAELAGLSFAIGAFVAGVSLATSPIAQYIAENLRPLRDFFLVLFFF
                     SVGAGLDVMLLMDVFFPTLLLALVLV"
BASE COUNT         1774 a         3212 c         3037 g         1718 t
ORIGIN      
        1 agacgatccg cctcctccag cagggccggc agcgctcgag agcgcagctt gccccgcatg
       61 ctcggaatga tgcagaagct gcaggtgtgg ttgcagccct cggcgacctt gagataggcg
      121 tagtgcgcgg gagtcagctt cacgccggcg gggccaaggt ggtagtgcgt ggactcagca
      181 gcgctcagcg ggacatgggc ccgcacggcg tccacgacgg gaaccgggtc ctgaggaccg
      241 ctgaccgaca ggagctccgg gtaacgttcc tgcaggctgt ctttccgcgc gccgaggcag
      301 ccggtcacca ccaccttgcc gcaggcgtcc agggcctctt cgatggcgtc aaacgactct
      361 tcgatggccg cgtcgatgaa accgcaggta ttgacgacca ccacgtcggc ttcggagtaa
      421 tcatcggccg tggtataccc ctccgccgtc aacgccgtga gcagctgctc cgagtcgacg
      481 agggccttgg gacatccgag gctgatgaac cccacctttc ctgtcatctt ctagttgccc
      541 ggcaccggtt gtgaagcgtc gcgcattgta gccgtggccg gtcgtcgggt cactgaacca
      601 ctccagatgg acgatgcagg cgcatgcaga cgcaccgagc gcgagttgat tttcccggtc
      661 gagtgtgtaa ccgtccgcgc gccatggaag acaacgcacg acaattctgg ttccaggtct
      721 ggtcccaagg ctccaacgcc gttggcggca cgggcagcca gggttggtat tggtttttta
      781 acggatagca ccgccttcta gaaaagggag gcagtgcagg ctgccttcgc agggaacccc
      841 gcaaggcagc gccagcgggg ttttttttgc cctgatgaag gcgtttcgag gagacgatga
      901 tgtcggcaga actagagaac ctgaacgtcg cggagacgga ggtgctggtc acccccgccg
      961 cgctcaaggc gctgttgccg gtgcccgacg cggtcggcga gagcgtgcgg gcatcccgtc
     1021 gggcggtgca gaacattctc gaccactcgg atcaccggct gctggtcgtg gtcggcccct
     1081 gctccatcca cgacacggac gccgcccggg agtacgccca gcgtctgaag cagctggccg
     1141 acgaagtgtc ctccgaactc ttcgtcgtga tgcgcaccta cttcgagaag ccgcgcacca
     1201 ccgtcggctg gaagggattg atcaacgacc cgtacctgga cgactccttc agagtcggcg
     1261 agggcctgaa gattgcgcgc cggctgctgc tggacatcac cagcctgggc ctgccggtga
     1321 gcaccgaagc gctcgacccc atcaccccgc agtacctcca ggacctgatc acctggtcgg
     1381 ccatcggtgc ccgcaccacc gagtcgcaga ctcaccggga gatggcgtcg gggttgagct
     1441 gcccgatcgg tttcaagaac ggcacagacg gctccctgga cgtcgcgatc aacgctctgg
     1501 aatccgccgt gagcccgcac cgttttctgg ggatcagctc atcgggacag gtctcggtga
     1561 tccgcactac cggcaaccgt catgcccaca tcgttctgcg cggcgggagc cgggggccca
     1621 actacgacgc cgagagcgtc gcggcctgtg aggcggccct ggagaagatc ggccaggttc
     1681 ccaacatcat ggtcgactgc agccatgcca actccaacaa ggaccaccgc cgccagccgg
     1741 acgtggcgcg aagcttagcc gagcagatcg agaacgggag ccgctccctg atggggctga
     1801 tgatcgagag caaccttcac gagggcaacc agcccgtcaa cggcgggagc aacctccgct
     1861 atggcgtatc ggtgaccgat gcctgcatcg attggccgac aacggagtcg ttgctgcggg
     1921 agcttgccga gcggctgggc cccgcactgc gggcgagagc caaagtggct caacggtagt
     1981 aggcgtgctc cgtcatcgtg tggtcggtgg cgtccttgat gcccgtcagc tcgggaatct
     2041 gctcgaggag agatttctcg acgccgtgct tgagcgtgat gtccaccgcc gcgcagccct
     2101 gacagcctcc gccgaactgc aggatcgcgg tcatgtcctc gagaacctcg acgagggtca
     2161 ccacgccgcc gtgggccgcc agaccgggat tgatctcgtt gtacaggaca tagttgatgc
     2221 gatcctcgag cggcgcgtcg ggaccgaccc ggggaagccg ggcattgggc gccttgatgg
     2281 tcagctgccc acccatccgg tcctcggcgt agtcgaccat ggcttcgacc aggtacggcg
     2341 cgctgcgttc ctcgaaccag gcggtaaatc cgtcgtaggc gaccgccacg tccccttcct
     2401 gctgctctcc gggccggcaa taggcaatgc aggtctccgc ctgaggggtg ccgggctggg
     2461 ccacgaacac gcggatgccc acgccctcct cttcctggtt ggccagcaga tcgcgcaggt
     2521 acccttgcgc cgactctgaa atctcgatca tggctaggtt ccggtgggtg cttgagctat
     2581 acctgagtaa aacactcaga atagaagcgg catggtagct gaaccccggg ggccgggcaa
     2641 tcgcgcatga gcttgactca tgatcatctc cgataacttc gattgcgctc aggttggcgg
     2701 cggctagact gatacccatc aacccatgcc cgaagactcg atgcctgccg agccctcact
     2761 gattcagcaa ctcaaggagc gcatcctgct gctcgatggt gcctacggga ccatgctcca
     2821 ggccttgaat ctcgatgagc cagactatcg gggctcgatc ttcgagggcc atccggctcc
     2881 gctgaagggc aactacgatg ccctcaatct ggcccgtccc gagctggtcg cagacgtcca
     2941 tcgcgcctat ctgggcgcgg gggcggacat catcaaaacc aacagcttca actccacgag
     3001 aatcgctcag ggcgactacg cgctggccga tcgcgcggag cagcttaacg ccgccgccgc
     3061 acggatcgcc cgacgctgtg ccgatgagtt tgcctcgccc gaatggccgc gcttcgtcgc
     3121 gggcgtgctc gggcccacca accgcacggc gagcctctct ccggacgtga acgatcccgg
     3181 gttccgcaac gtcaccttcg aggagctaga ggagaactat tacgaagccg cgcaagcgct
     3241 tctcgacaac ggtgcggatc tgctactgct cgaaaccgtg ttcgacacct tgaacgcgaa
     3301 agcggcgatt ttcgcgatcc gccagctggg cgagaaacgg ggcgcgacgg tgccgctcat
     3361 cgtctccgga acgattaccg acgccagcgg gcgaacgctt tccggccaga cgtgcgaagc
     3421 gttctgggcc tccgtcaatc acgccgagcc cctggccgtg gggctgaact gcgcgctggg
     3481 agccgatcag ctgcgcggct acgtggcgcg cctggggcgc tttgccgata cctacgtcag
     3541 cgtccacccc aacgcgggcc tgcccaacgc gttcggcgag tacgatgagt cgcccgagtc
     3601 catggccgcc aagctcggcg agtttgcccg cagcgggctg gtaaatctgg tgggcgggtg
     3661 ctgcggcacg acgccggcgc acattgccgc cattcgagag tcggtgcggg aatgccagcc
     3721 cagagcgata cccgagcgcc gcaacgatct gatgctcagc ggcctggagc tgctcaccat
     3781 cgacgacgcc tcactcttcg tcaacgtggg cgagcggacc aacgtgacgg gctccgcgcg
     3841 gtttgcccgg ctgatacgcg aagaggccta cgacgaagcg ctcgtcgtag cccgccagca
     3901 ggtggagaac ggggcccagg tcatcgatat caacatggac gaggggatgc tggacagcca
     3961 ggccgccatg gtgcgctttc tccagctcct cgccgccgag cccgacatcg ctcgcgtgcc
     4021 gatcatgatc gactccagca aatgggaaat catcgaggcc ggtcttcgct gcgtgcaggg
     4081 caaggcaatc gtcaactcga tcagcctcaa agagggagaa gacgccttcg aggccgtggc
     4141 tcgacgatgt cgcgactacg gcgcggccgt ggtggtgatg gccttcgacg aggacggcca
     4201 ggccgacacc ctggagcgca agcgcgagat ctgcaagcgt tcctaccgca tcctgacgga
     4261 gcggatcggg ttccctcccc aggacgtgat cttcgatccc aacgtctttg ccatcggaac
     4321 cggcatcgac gagcaccgcg gctacgcggt cagcttcatc gaggcgtgcc gctggatcac
     4381 cgacaacctg ccccacgcga gagtctcggg cggcatcagc aacgtctcct tctccttccg
     4441 cggcaacaac cccattcgcg aggcgatcca cacggtcttc ctgtatcacg ccattcgcgc
     4501 cgggctgacc atgggcatcg tcaatgccgg tcagctggga atttaccagg agattcccga
     4561 tgagctgcgc gagaaggtgg aagacctggt gctcaatcgc cgatccgatg ccaccgagcg
     4621 tctgctggcg gttgccgacc ggtacggcgg cagggcctcg gagagcgaag ggcccgacct
     4681 ttcctggcgg gaaggcagcg tggcccagcg actcggccat gccctggtga aaggcatcgg
     4741 cgagttcatc gtcgaggaca ccgaggaagc ccggctcgcc gccgatcatc ccatcgaggt
     4801 gatcgagggc cccctgatgg acggcatgaa cgtggtgggc gacctgttcg gccaggggaa
     4861 gatgtttcta ccccaggtgg tcaagagcgc gcgcgtgatg aaacaggcgg tggcccacct
     4921 ggtgccctac atcgaagccg cacagggccc gggtcggaaa gccaaaggcc gcatcgtcat
     4981 ggcgaccgtg aaaggagacg tccacgacat tggcaagaac atcgtcggcg tcgtgcttca
     5041 gtgcaacaac tacgaggtga tcgacctggg cgtcatggtg cccatggaga aaattctcga
     5101 cactgcccag gaggtgggcg cagacgtcat cgggctttcc gggctgatca ccccctcctt
     5161 agacgagatg gtgcaggtgg ccggcgagat gcaaagacgc ggcctgaacc tgccgctgat
     5221 gatcggtggc gcaacgacct ccaaagccca cacggcggtc aggatcgagc cggcttacca
     5281 ggatggcccc accgtctacg tttccgacgc ctcgcgcagc gtgagcgttg ccgcacgcct
     5341 ggtctctcca cagcagcgca tcggcttcag cgaggagacc agaaaggaat acgaggagat
     5401 ccgccggcgc acgtctgccc gctcggccaa ccggaagtat ctcagcctgg aggccgcccg
     5461 acgcaacgct ttcgtctgca actgggaggc ctacgaaccg cccagaccgg cctcgatcgg
     5521 cgtccagacc atcgagaacc tgcccctgga gaccctggtt ccctacatcg actggtcgcc
     5581 gttcttccta acctgggaac tggccggcaa ataccccgcg atcctggacg atcccgtggt
     5641 gggcgagagc gcccgggatc tgtttcgcga cgcctcgcag atgctcgaac acctctgccg
     5701 cgagaagcgc ctgaaggcga acgccgcgtt tggattctgg ccatgcaacc gcacggggcc
     5761 cgaggagctg accttgttcg aggatgagga gcgcaaaacg gagctcgcca ggctgcagca
     5821 cctccgacag cagacgatca ggggcgcggg gcgcccgaac tacagcctgg ccgacttcgt
     5881 gggaccgccc ccggtggccg actacatcgg cgctttcgcc gtgaccgccg ggatcggcat
     5941 cgaggacgtt cttgccgact acggcgacga cgactacggc gccatcatga tcaaggccct
     6001 tgccgacagg ttggcagagg cgtttgccga gtacctgcac gagcaggtcc gtcaagtgca
     6061 ctggggttac gcgcccggtg aatccctggc agccgaggag ttgatcggtg aagcctaccg
     6121 gggcatccgg cccgcgcccg ggtatcccgc gtgccctgag cacagcgaaa aaagtacgct
     6181 gttccgcctg ctcgacgccc aggctaacgc cggcatgacg ctgaccgaaa gcttcgccat
     6241 gctgccggcg gcctcagtag cgggctggta tttctcccac cccgacgcac gatatttcgg
     6301 catcggcaag atcgacgacg agcaggttgc cgactacgcc agcaggaaag gaattaccgc
     6361 cctggaagcg gagaagttgc tcaggcccaa cctcgatcgc tagaaatcga aggtccagcc
     6421 ggagacgtag tgcacggccc cggcgacaag ggcccagaaa atgatcatca cggcccaggc
     6481 gtcagccttg cgatccgctt cagatcttgc atcgtctgtg ggggtgtcgt ccatcgtgct
     6541 cctcaccagc gagttgatcg tgcccagcgc acggcgcgcg agtctaccga aaaagacggt
     6601 tctaagcata gtccgaacgg ttacttcgag cggcaagggc cgaatgttag attcgcctgc
     6661 ctcaaagatg acgagacgcg gcctgcgctt cgcgctgagc gccgattacc agccagccag
     6721 atgtatcgat acgacgactt cgacaggcag ttcattgcgg aacgcgttgc ccattaccgc
     6781 gagcagacaa cccgctatct tgccggcgag ctgtccgaga gcgagtttct gcagctcagg
     6841 ctccgaaacg gcctgtacat tcagcgactc gctccaatgc tgcgggtggc cgtcccctac
     6901 ggcacgctca gctccggcca gctgcgcaag ctggcccata tcgcgcgcca ctacgaccgc
     6961 ggttacggtc acctcagcac gcgtcagaac atgcagttca actggcccga gctcgccgag
     7021 gtacccgaca tcctcgcaga gctggccgag gtcgacatgc acgccatcca gaccagcggc
     7081 agctgcatac gcaacgtgac gaccgaccag ttcgccggcg tcgcggctga cgaggaagtc
     7141 gatcccagac cgtactgcga gctcatccgc cagtggtcga ccaatcaccc ggagttcgac
     7201 tggctgccgc gcaagttcaa gatcgccgtc accggggctg cttccgaccg agcagccatc
     7261 gccgtccacg acatcggctt gcgcgcgcgc cgcgaggacg ccgagggcat cgtgttcgac
     7321 gtgttcgtgg ggggcggcct cgggcgcacg cccgtcatcg ccgccgagat ccgcaagtcg
     7381 cttccggtga ggcatctgct gacctacctc gaggccatca tgcgcgtcta caaccgctac
     7441 ggccgcagag acaacaagta caaggccaga atcaagattc tggtacgggc catgaccgcg
     7501 gagggtttcc gcgaccgggt ggatgccgag tgggcccatc tgaagggctc ggaaagcacg
     7561 ctgaccgatg aggaaatccg ccgcatcgag gcttttttca ccccccacga gtatccgccg
     7621 ctgaacgatg ccgatctggg acccgcgcgc ctggaggatc ccgcctttgc tgcctggctg
     7681 cgccacaacg tctctcccca ccgggtaccc ggctacgcca tcgttacgct gtccacgaag
     7741 gtgacgggcg tgcccccggg agacgtctcc gacgtgcaga tggaggccgt ggccgactgg
     7801 gcagacgagt acgggttcgg ggagctgcga ttgggccacg agcagaactt cgtgctgccc
     7861 gacgtccccc agagccggct tcgggaactg tggcgaagaa ccgcggcgct ccagctgggc
     7921 gtcgccaacg tcgggctgat ttccgacatc gtctgctgcc ccggcggcga ctactgctct
     7981 ctggccaacg ccaagtccat tcccgttgcg gaagccattc agcagcgttt cgacgattac
     8041 gactaccagc acgacctggg tgagctcgac ctgaacatct ccggctgcat gaacgcctgc
     8101 gggcaccacc acgtgggtca catcggcatc ctgggcgtcg acaagaaagg cgcggagtac
     8161 taccagatct cgctgggcgg gcattcgggt gccggcggag acgaagcgtc tctcggcgag
     8221 atcatcggcc catccttttc ccgcgccgag gttcccgcga ccatcggcag gatcctcgac
     8281 gtctacctga aaaggcgcat cgacgacgag cctttcctgc actgctatcg ccgcatcggc
     8341 atccaacctt tcaaggatca cgtctatgaa cgcgcagctt gactacatcg accaacccgc
     8401 cgcgcagatg gtgtctggac cgggagccgt gccgggtccg gcgaggctgg ttgccgccga
     8461 gcaatggacg cccggatcgt ccgacggtct gctcctcgcc gtcgacgctg aaccggagca
     8521 gcggtttgcc ggggcggaac tgatcgccat cgactttccg gcattcacgg acggccgcgg
     8581 actctcgctg gccgtgctgc tccgaacgcg tctcggtttc cgaggcgagc tgcgcgccgt
     8641 gggcgacatt cgggctgaca tgctgcacta cctttatcgt tgcggcttcg acagcttctc
     8701 catgtccggc gagggcagcc ccgtagacgc cgacggcgtg accaagacca cggccttcga
     8761 cgcccttgca ccctactcgg attgctatca agcttcggta cgccagcccc gtccagctta
     8821 ccgaagggtc gagaggtgca tagcacagga attgtcgagt ccttctttct gatattcacg
     8881 ggcgccgcgg tgctagccac cgcggccctg tactcgcgcc agccgctgct cgtcgcctac
     8941 atcgccgtcg gtgctctgct gggacctcac gggttcaagc tgatttcaga ctctaacctg
     9001 ctttcggaaa tctccgagat cggcatcatc tttctgctct ttctcgtggg cctggacctt
     9061 caacccagca agctgaagaa catggtgggc gaatccctgc tgaccgccgt gggcaccacg
     9121 atcgtgtttt tcgccgcggg tttcgcgatg atgctcgcct tcgacttttc cacgagcgag
     9181 gccgtggtca ccggcatcgc ggtgacgttt tccagcacca tcatcggcat caagctgctc
     9241 cccaccacgg ttctgcatca ccgtcatacg ggcgagatcg tcgtcagcct cctgctgatt
     9301 caagacctga tcgccatcct cgctctcatc gtcttaacgg gctacggcga tacgttgggc
     9361 gccacgctgt cgagcctcgg gatggcgctc atcgcgctac cgctgctcat cctcggcgcg
     9421 ctcggcggca tgcgcctgct ggtattgccg ctgatcagaa aattcgacgc cttcaacgag
     9481 ttcatttttc tgctggcgct gggctggtgc ctgggccttg ccagcatcgc ggagcttgcc
     9541 ggcctgtcgt ttgccatcgg cgctttcgtg gccggtgttt ccctggccac cagccccatc
     9601 gcgcagtaca tcgccgagaa cctgcgccca ctgcgagact tctttctggt tctcttcttc
     9661 ttctcggtgg gagccggtct cgacgtcatg ctcctgatgg acgttttctt ccccacgctg
     9721 ctgctggcgc tggtcctcgt t
//
LOCUS       DBNHLM010000162         3676 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_741123, whole genome shotgun sequence.
ACCESSION   DBNHLM010000162 DBNHLM010000000
VERSION     DBNHLM010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_741123"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /gene="leuB"
                     /locus_tag="AC16QF_07405"
     CDS             <1..682
                     /gene="leuB"
                     /locus_tag="AC16QF_07405"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003116443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR6448420.1"
                     /translation="GIYFGEPRGIEVVDNERRGFNTYVYSEHEIERIARVAFELARKR
                     DRRVCSVDKANVLEVTQLWREVVSEVAGDYPDVELSHMYVDNAGMQLVRAPKQFDVVV
                     TGNMFGDILSDVAAMLTGSLGMLPSASLSSDGRGLYEPVHGTAPDIAGQDKANPLATI
                     LSVAMLFRYSLDAPEAAWAIESAVDAVLAAGWRTADILQAEDSSKLRLIGTREMGDRV
                     LEQLETGA"
     gene            705..1757
                     /locus_tag="AC16QF_07410"
     CDS             705..1757
                     /locus_tag="AC16QF_07410"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF005957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR6448421.1"
                     /translation="MSSGVNVAIAGATGAVGEALLEVLEQRKFPVARLSLLASERSEG
                     KRLQFRGKSVPVERLDNFDFSDTAIAFFSAGGDLSAQFAPKAAAAGCIVVDNTSQFRY
                     DADIPLVVPEVNAHRLDQPNPRNIIANPNCSTIQMVVALKPLHDVARIRRINVATYQA
                     VSGAGASAIRELAGQTASLLNGKPIESKVFPVQIAFNALPHIDKFQDNGYTKEEMKMV
                     WETQKILEDEAIRVNPTCVRVPVFFGHSEAVHIETEHKITAEEARRLLAAAPGVTLID
                     DPSANGYPTAVSHGAGQDAVFVGRVREDVSFDRGLNLWVVSDNVRKGAALNSVQIAEE
                     LMARQPQLFAISDGLG"
     gene            2062..>3676
                     /locus_tag="AC16QF_07415"
     CDS             2062..>3676
                     /locus_tag="AC16QF_07415"
                     /inference="COORDINATES: protein motif:HMM:NF051932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0044096 - type IV pilus [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein FimV"
                     /protein_id="HIR6448422.1"
                     /translation="MIRSLLSRARAHVISIGAALSLLAAHAWGLGLGEIKLNSALNEK
                     LKAEIELLDASDMQPDEIRVSLASTEDFERVGVERFFFLTDLRFEVAFSSSSGVKVDV
                     TSTKPITEPYLNFIVEVLWPNGRLLKEYTLLLDPPTFSSAAAPVVAPPARTASSGGSG
                     DDVTRTAPSGGTRVSLAPPSPSTPAPRNIGGELMTDQSDTLWEIAQRTRPEGVSIAQD
                     MLAIQKLNPQAFIRNNINLLKAGYSLRLPTAEEARALSQQQAVALVAEQNADWRAYNR
                     GEAPAVETQVAQSVPSEFQGQVDATAERAEPVVESASEGELRIVAGVGDSVSGTAAAE
                     TVAQLDAAREEQDRLSREVDELAYQMDRERELSTNQLAVKDRQLEVKDQQIAELQAQM
                     AQVRQELNEARQNQNQSVPAPEQAKSWWESSYVLGGAAGALVLALVAGLIGARRKRPV
                     EDEVAVAAVTPRAARTSTTAAAAKAATADEGASVQAASGVSGDSGAHDAVPAPGDGDV
                     LAEAEIYIAYGRLPRAISLLQDAIEEEPER"
BASE COUNT          685 a         1141 c         1210 g          640 t
ORIGIN      
        1 cggcatctat ttcggcgagc cgcggggcat cgaggtggtg gacaacgagc gtcgtggttt
       61 caacacctac gtttactccg agcacgagat cgaacgaatc gcccgggtgg ccttcgagct
      121 ggcgcgcaaa cgggatcgca gagtctgctc ggtcgacaag gcgaacgtgc tcgaggtcac
      181 acagctctgg cgggaggtcg tatccgaggt ggccggcgac tatccggacg tggagctcag
      241 ccacatgtac gtggacaacg ccggcatgca gctcgtccgc gcgccgaagc agttcgacgt
      301 ggtggtgacg ggcaacatgt tcggcgacat cctgtcagac gtggccgcca tgctgaccgg
      361 ctcgctgggc atgctgcctt ccgcgtcgct gagcagcgac ggcagagggc tctacgagcc
      421 cgtccatggc acggcgccgg acatcgcggg gcaggacaag gcgaatcccc tggccaccat
      481 tctctcggtg gcgatgctct tccgctacag cctggacgcg ccggaagccg cctgggccat
      541 tgagagcgcc gtggatgcgg tgctcgcggc cgggtggcgg acggccgaca ttctgcaggc
      601 cgaggactcg tccaagctcc ggctcatcgg tacccgggag atgggtgaca gggtgctcga
      661 gcagctcgaa accggggcgt gacaggcaat tggaggcgga tgccgtgagc agtggagtca
      721 atgtggccat cgccggggca accggcgccg tgggcgaggc actgctcgaa gtgctggagc
      781 agcggaagtt tcccgtcgcc cgcctttcgc tgcttgccag cgagcggtcc gaaggaaagc
      841 gcctgcagtt tcgcggaaag tccgtgcccg tggagcgtct ggacaacttc gatttttccg
      901 acacggccat cgcttttttc tccgccggcg gggacctctc ggcgcagttc gcgccgaagg
      961 cggcggctgc cggctgcatc gtcgtcgaca atacctccca gttccggtac gacgcagaca
     1021 tcccgctggt cgttccggag gtaaatgccc atcgtctgga ccagccaaat ccgcgcaata
     1081 tcattgccaa cccgaactgc tcgacgatcc agatggtggt ggcgctcaag cccctgcatg
     1141 acgtggcccg aatccgtcgc ataaacgtcg cgacgtatca ggcggtgtcc ggtgctgggg
     1201 cgagcgccat acgggagctt gcgggtcaga cggccagtct gctgaacggc aagcccatcg
     1261 agagcaaggt cttcccggtg cagattgcat tcaacgcgct gccacatatc gacaagtttc
     1321 aggacaacgg ctacaccaaa gaagagatga agatggtgtg ggaaacccag aagatcctgg
     1381 aggacgaagc gattcgggtg aatccaacct gtgtcagggt gcccgtcttc ttcggccact
     1441 cggaagcggt gcacatcgaa accgagcaca agatcacggc ggaggaagcc cgccgtctgc
     1501 tggccgcagc acccggcgtc acgctgatcg atgatccctc ggcgaacggc tacccgacgg
     1561 cggtgagcca cggcgcgggc caggatgccg tgttcgtggg gagggtgcgc gaggacgtct
     1621 ccttcgatcg cggtctgaat ctgtgggtgg tttcagacaa cgtccgcaag ggcgctgcgc
     1681 tcaacagcgt gcagatcgcg gaggagctga tggcgaggca gccgcaactc tttgccatat
     1741 ccgatggttt ggggtaaaag gctccagcaa cttaaccgta ccgacacatc cttcatgccc
     1801 gccagccgcg tcggagtcgt ttcgtcagat cccgcagcaa gctgcctgca gaacggcttt
     1861 cggggcgtcc cgattcggga ctcagcgccc aaaaccatcg cgaaactgtt acctactgca
     1921 atttttggcc gtcccaagtg aggtatcgtc tcgtttggca catggatttg atgaagtgca
     1981 caggatagca gtgagacatt cactcggcgg aacgggcgtc cgcatcaatt tcgctattac
     2041 ttttgttaaa ggggctagcc tatgatcagg agtctgctca gtcgcgcgcg cgcgcacgtg
     2101 atttctatcg gtgccgcgtt gtctttgctt gcggcacacg cctggggcct cggcctcgga
     2161 gagatcaaac tgaactcggc gctgaacgag aagctcaagg cggagatcga gttgctcgac
     2221 gcgtcagaca tgcagcctga cgagatccgg gtgtctctcg cctctaccga ggatttcgaa
     2281 cgggttgggg tcgagcggtt cttcttcctg acggacctgc gcttcgaggt cgcgttctcg
     2341 tcctcgtccg gcgtcaaggt cgacgtcacg agcaccaagc cgataactga gccctacctc
     2401 aacttcatcg tggaggttct ctggcctaac ggcaggctgc tcaaggagta cacgctgctc
     2461 ctcgatccgc ccacgttcag cagcgcggcg gctccggtgg tggcgccgcc cgcacgcacg
     2521 gcgtcctccg gcgggtccgg agacgacgtg acccgcacgg ccccatccgg cggtacccgc
     2581 gtgtccctcg cgccgccgtc gccctcgacg cctgctccgc ggaacattgg cggtgagctg
     2641 atgaccgatc agagcgatac gctctgggaa atcgctcaac gcacccggcc ggaaggcgtg
     2701 tccatcgctc aggacatgct cgccatccag aagctgaacc cgcaggcgtt cattcgcaac
     2761 aacatcaacc tgctcaaggc cggatactcg ctgaggctgc ccactgccga agaggcgcgc
     2821 gcgctgtctc agcagcaggc cgtcgccttg gtggccgagc agaacgctga ctggcgcgcc
     2881 tacaatcgtg gcgaagcgcc cgccgtggag actcaggtgg cccagtccgt gcccagcgag
     2941 ttccagggtc aggtcgacgc gaccgcagag cgggccgagc cggtcgtgga atccgcatcg
     3001 gaaggagagc tgcggatcgt cgccggggtc ggcgacagcg tatccgggac cgccgctgcg
     3061 gagacggtcg cgcaactcga tgcggcacgt gaagagcagg atcgcctttc cagggaagtc
     3121 gacgagctgg cctaccagat ggatcgggaa cgtgagcttt ccacgaacca gctggcggta
     3181 aaggatcgac agctcgaggt caaagaccag cagattgccg aacttcaggc tcagatggcc
     3241 caggttcgcc aggaactcaa cgaggcgcgg cagaaccaaa accaaagcgt gcccgcgccg
     3301 gaacaggcca agagttggtg ggagtcgtcc tacgttctcg gcggtgccgc gggcgctttg
     3361 gttttggctc tcgttgccgg attgatcggc gcgcgtcgca agcgccccgt cgaggacgag
     3421 gttgctgtcg ccgccgtcac ccccagggca gcgcgtacca gcaccacggc tgctgcagcc
     3481 aaggccgcca cagccgacga gggagcctca gtccaggcgg cgtcgggcgt gtccggggac
     3541 agcggagcgc atgacgcggt gcctgcacca ggtgacggcg atgtccttgc cgaggccgag
     3601 atctacattg cctacggtcg tttaccgcgg gccatcagcc tcctgcagga tgctatcgaa
     3661 gaggagcctg agcgcg
//
LOCUS       DBNHLM010000163        12128 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_442347, whole genome shotgun sequence.
ACCESSION   DBNHLM010000163 DBNHLM010000000
VERSION     DBNHLM010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12128
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_442347"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..506
                     /locus_tag="AC16QF_07420"
     CDS             279..506
                     /locus_tag="AC16QF_07420"
                     /inference="COORDINATES: protein motif:HMM:NF015735.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR6448423.1"
                     /translation="MGAITGIAGRPVQVRNPEGQALETIQGPYMSTELPIAGFSVIEA
                     DSLEEAIELVSQTPCAVAYGVVEVWPLETIS"
     gene            complement(611..1636)
                     /locus_tag="AC16QF_07425"
     CDS             complement(611..1636)
                     /locus_tag="AC16QF_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003158442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR6448424.1"
                     /translation="MKTIYSERHRLRDAKTELYGGELVQPFERPSRADMVIRAVRQSG
                     LGEIDGPDTFSLDPVLAVHDTEFVSFLASAWQEWQQTGYKGEAIATVWPARRMQDRVP
                     RFIEGKLGYYALAAETSICEGTWAAALAAKDVALTGARGLIEGERGMFSLCRPPGHHA
                     ARDLFGGYCFLNNAAIAAQYLRDHDAERVALLDIDFHHGNGTQDIFYERDDVLFCSLH
                     GDPQDAFPHFLGYADETGLGAGIGFNRNYPMPPGTRFVQWRQSLSDALTHIGEFSPQY
                     LVVSLGVDTFENDPISFFKLTTPDYLTIGEMIGGLGIPTLFVMEGGYDIDEVGINTVN
                     VLTGFAA"
     gene            complement(2288..2608)
                     /locus_tag="AC16QF_07430"
     CDS             complement(2288..2608)
                     /locus_tag="AC16QF_07430"
                     /inference="COORDINATES: protein motif:HMM:NF020317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR6448425.1"
                     /translation="MPKYLLEVDYSAAGNAGLVEGGGTARQQTVENMVNGLGGKVESF
                     YFTFGIRDAIVIADLPDNVTAAAISIACSATGSVAYKTTVLLTPAELDAASKKTVAYS
                     PPGA"
     gene            complement(3083..3616)
                     /locus_tag="AC16QF_07435"
     CDS             complement(3083..3616)
                     /locus_tag="AC16QF_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448426.1"
                     /translation="MDIDRVGSLMGNFGEFFGSIAVVVTLIYLALQVRQNSQALRIGA
                     RQHVLDRLSDAIRHIVSEPDTRAVITKAFNQIDDLTQDERVHFNHLMMLFSNNFYDAL
                     LLYRDSDLNDDAFNLVANAYVGSCATPGGNAWWKQMSEAAGDTGINFPPDFIEYVNRR
                     LEEDTGSGRTVGAVWQL"
     gene            complement(3776..4648)
                     /locus_tag="AC16QF_07440"
     CDS             complement(3776..4648)
                     /locus_tag="AC16QF_07440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6448427.1"
                     /translation="MAFSSKPNFGVTIFPTDYTIQPAALAQAVEERGLDSLFFPEHTH
                     IPASRATPFPGGTELPRMYWHTHDPFVALGACAAVTSRIRLGTGICLVIERDPITLAK
                     EAASLDMLSNGRLVLGIGAGWNREEMENHGADYKNRWRIVREKVLAMREIWANEAAEY
                     HGEFVDFDPIWSYPKPVQPGGPPIWIGANSKWVFDRIADYADGWMPIGGIGSGNMERL
                     SEALAARGRKLEDVDMALFGAPTDYDQLMGRIEQGFDELVFALPDADADTVLPMLDDL
                     AELAGKARGAGRTA"
     gene            4838..5590
                     /locus_tag="AC16QF_07445"
     CDS             4838..5590
                     /locus_tag="AC16QF_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448428.1"
                     /translation="MDWRTWLGLSLTIAWLLLGALYVSDQIGWSQFAALNADALGSFL
                     EGAFAPLAFLWLVIGYFLQQKELQQNTRALEAQAQEIQRSAEQAVIQSEKMAESEMHA
                     RQQAFLQIAESVKRQLGSIGGMLFISSQGSDAEGNVTAEEFSELFSMKAGQDPEIFSR
                     RLLESHTRARPERQLAIFYGTPVRARHSNNFIYTFERLLARAELVDTDNMIRDSLLGS
                     AHGFVYSLAKRHRAAAPEELADPSQTGTDIRF"
     gene            5865..6419
                     /locus_tag="AC16QF_07450"
     CDS             5865..6419
                     /locus_tag="AC16QF_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448429.1"
                     /translation="MTRIAGDLAHNTGLGEALVGALFIGASTSLSGITTSVSVAAAGH
                     AELAVSNGLGGIAAQTSFLALADIVCRRANLEHAAASAENLFMSAFLLTLLGIHALAL
                     AIPDVGIFGAHPATVILVIAYVFGVHLLARTHDLPMWLPIRTADTPTETADHNVRRRH
                     QRRAVLACQLDGDDRRAAHGSHLS"
     gene            6768..7235
                     /locus_tag="AC16QF_07455"
     CDS             6768..7235
                     /locus_tag="AC16QF_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6448430.1"
                     /translation="MQGPVRTMTLDEVRSFTGKEIGVSDWFELDQSKINQFADLTEDH
                     MFLHVDPEAAKATPFGSTIAHGLLTLSMMPVMAYQAMPGVSGTKMGVNYGYNKVRFMA
                     PVKSGKRIRGHFVVQGIEDKGDGRQQITHDVSIEIEGESKPALVAEWMTMVWM"
     gene            7270..7995
                     /locus_tag="AC16QF_07460"
     CDS             7270..7995
                     /locus_tag="AC16QF_07460"
                     /inference="COORDINATES: protein motif:HMM:NF022041.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6448431.1"
                     /translation="MAILELVTLPPAFGLRNVSPFCLKAEMLLKSLDLPFAQSQLQDP
                     RKAPKGKLPYLLSDGVKIADSELITEHLDEMTQGRVYAGLSPEQKAQGVALTRLVEDH
                     LYWIGVASRWLDDAWWPHVVENFFGFVPALIRPFAAGGARKQVRQTYHLQGLGRHTLE
                     EQRGFAKRDLQALEDAVPRTGFLFGDAPGIFDFTVAGMMAGFYDNQPPTWLTELALTY
                     PGLNEYTERVQEAIGVYGRHVDA"
     gene            complement(8018..9403)
                     /locus_tag="AC16QF_07465"
     CDS             complement(8018..9403)
                     /locus_tag="AC16QF_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450 family protein"
                     /protein_id="HIR6448432.1"
                     /translation="MSSESEFVKASKGDPVRILGKSRPSLSRSGMPLFARNGTAADYR
                     TYEIYQRERVHESTEKIRPIELVSSEFVRDPYPVLGVLRENYPCYRDWLGNAYWLTRY
                     NQVTSVFTDDANFETRPKLWFYRMQGYGRDLRSELPMQIAHERLIDANAVPVAEEIVS
                     AFAARGSADLALEFAARLPMELLAKVLDLPAEDFHWFVERYWRMQRGFDWNAADEQAG
                     KRAMTELADYFRPLLEARRADPGDDMVSAIATLELEDGPTTAEDVVVTLLEADHETMH
                     GALANFWFLLLTHPDQLEQVAQERRLLKFAYLEALRHSTPVLWKKRFALHEVERFGRL
                     LPIGGLFYCAAAAANRDPWIFSDPDEFDITRRDLCQREPRGHYRSDGLAAGVALGLGR
                     PSPYPGIPEDRPRSLYAMTRDTIVTASNILLDTLPDIRLQTQAQPELRCLRLGEMHTC
                     WHLPAEFTPRS"
     gene            complement(9407..10858)
                     /locus_tag="AC16QF_07470"
     CDS             complement(9407..10858)
                     /locus_tag="AC16QF_07470"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6448433.1"
                     /translation="MSTESAFADTLRRTIGAETIAGDADPGAKIDFPLMPLGEAADMG
                     RAEDGTPLIDPRIFYSAEFRRNPWPYYRILRDHYPVYFNKLNNTYYVTRYADMTECYY
                     DDIGFNTIPKGSSNGVLGNVTHEFSGVEHRRRRNLFGQHLVGKSLEHRVPAIERLALE
                     MIDDFANLDESVRQVSGKVATIELGRAFAYEFPVRVVCDVLGFPKEAQAHFFYWYDSI
                     MSGLGASETHKQGMEARQHLEDYVSELVEERRRSPGYLYDNQGNKLGPDIISKLCASK
                     IDGDYLSSVEITSYIALVVAGGGETTRGTIMNMWYSLLQHPEQFAAVVADDALWDAAF
                     HETLRHSNPIGGQPRHATFDVELHGVHIPAGSLIEMVNFSGSHDERVFKDPETFDIFR
                     DDLYSGKLLRSGYSKDGVCSHMAFGVGPHMCPGAWISHQEATIGSRLLAKRLKNARIN
                     RERMPKDIDGVSLAPIGINKLRDLWIDYDIDQP"
     gene            11179..11727
                     /locus_tag="AC16QF_07475"
     CDS             11179..11727
                     /locus_tag="AC16QF_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448434.1"
                     /translation="MDLNATVQLLGNLGEFVAAIAVVASLLFVGFQVRQSDRTQRTTS
                     LQSVLDGHRDRTFAPLYESEDMADLWARGLTSFDDLSETEKRRFFSFMFEQCFQMQQV
                     MQLHEQGLVPDVDFDAWLYYTGVLFNCPGGRAVWPIIERLVTPTVRDLMNEYWDENPT
                     LPTYQELNPLFVADRAAGVIGT"
     gene            11890..>12128
                     /locus_tag="AC16QF_07480"
     CDS             11890..>12128
                     /locus_tag="AC16QF_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448435.1"
                     /translation="MSEILGAEVTGFDVTFLEGGVLSDAFKVHDIAYGAPAGDQPASV
                     VVKLTNAVPERRAVAAQNKAYVKEVRFFKELADQVP"
BASE COUNT         2327 a         3582 c         3729 g         2490 t
ORIGIN      
        1 cggccttgtg ggtcattggg taaatcgatc ccactatggc aaacccgtcc ccatcagaat
       61 tgtctgcgtt tggccgatag cagatttcat ggcctctgga tggcgaggtc cgcgtcctac
      121 ccggagcagt cggatgctct ggacgaaact gaaggacttg gtagattgaa gccatggcga
      181 aattcataac aatcggatat ggcgaccagg aggggtacga ccgcaccaaa cgggaagttc
      241 ggactgccgc ccatgcacac gattcaaagc taatcagcat gggcgccata acgggaatcg
      301 caggtagacc tgttcaggtt agaaaccctg aggggcaagc gcttgagacg attcaaggtc
      361 catacatgtc tacggaacta cccatcgcgg gcttttccgt cattgaggca gatagccttg
      421 aagaagcaat cgagttggtt tcccaaactc cttgcgcggt tgcgtatggc gtcgtcgagg
      481 tttggcctct cgaaacgatt tcctagtatc cgaggattcg tgtggttctg gccgatagct
      541 aaagttttca ggcacttcga tccggcggca tatctctcgc gattcagcaa cgcatcattg
      601 ctcggggcgg ttatgccgcg aaaccggtca gtacgttcac ggtgttgatg ccaacctcgt
      661 cgatatcgta gcccccctcc atgacaaaca aggttggaat gccaaggccg ccgatcatct
      721 cgccaattgt caggtaatcc ggagtggtca atttgaaaaa gctgatcgga tcgttctcaa
      781 aagtatccac cccgagagac accaccagat attgcggtga gaattcaccg atgtgtgtca
      841 gtgcatcaga cagtgattgg cgccactgca cgaatcgtgt ccccggcggc atgggatagt
      901 ttcggttgaa gccaatacct gcccccaacc cggtttcgtc cgcgtaaccg agaaagtgcg
      961 gaaacgcatc ctgaggatct ccgtgcagcg aacagaacag aacatcatcg cgctcgtaaa
     1021 agatgtcctg ggtaccgtta ccgtgatgaa aatcaatgtc cagcagggcg acccgctcag
     1081 catcgtgatc gcgtagatac tgtgccgcaa tggcggcgtt gttcagaaag cagtagccgc
     1141 cgaacagatc tcgcgcggcg tggtgacccg gcggacggca cagtgagaac atgccccgct
     1201 cgccctcgat gaggccccgc gccccggtca gggcgacatc cttcgcggcc agggccgcgg
     1261 cccaagtgcc ttcgcagatc gacgtttcgg cggccagagc gtaatagccg agcttgcctt
     1321 cgatgaagcg cggcacgcga tcttgcatgc gcctggccgg ccaaactgtc gcgatcgcct
     1381 cacccttgta accagtttgc tgccactcct gccaggcgct cgccagaaac gaaacgaact
     1441 cggtatcgtg cacagccagc accgggtcga gggaaaaggt gtcgggccca tcgatctccc
     1501 caaggccgga ctgacgcacc gccctgatca ccatgtcggc ccgtgatggc cgctcaaagg
     1561 gttgaacgag ttcgcctccg taaagctccg ttttcgcgtc ccgcaagcga tgccgttcac
     1621 tgtatatcgt tttcaaagcg ccctccctgt gccgcctcag ctggcagctt ctttaatctc
     1681 ggtttgacgg cgtgcttcgt aatggctcat cccagtggtc gagcgataaa cacttcatcg
     1741 cccgactgac agggttccag gacaacgagc gcttgacggc cgtttgcgtc ccccaacccg
     1801 ggaacccagt gttaccgcag cgactcagca cccaaagaac agcaaaagcc ccgcctgcga
     1861 ccgcccgcat gggccctcgt caacctatca cccttcaatc gtacttccca gccagctcat
     1921 tccggcccgc tatttgtctt atgcgcaagc cccccgatcc cttacaacgc ttcaaagaaa
     1981 gcagggccaa ccggcagcgc agctttccct gaagcagatt cggcaggtct gcgacaagca
     2041 tgcgagtcgc tatgacctcg cgctctgtcc ggggtgattg ccgtaggtcc tcccggctac
     2101 atcggcgatc tcgacccggt gatcgacggg gctactcgtg ggctatcgtc aggcgggacg
     2161 atggttgtcc ttgatggcaa gctccccgag gacgagagag tagccctcga ggcgctgatt
     2221 gcggtcggcc tgaacaaccc agcgtttaac acatgagggc ggcagcggca ggtgccgaga
     2281 cctgatctca cgccccaggc ggcgagtaag ccacggtctt cttgctcgcc gcgtcgagtt
     2341 ctgcgggagt gagcagcacg gtcgtcttgt aggccactga gccggttgca ctgcacgcga
     2401 tcgaaatggc tgccgcggta acgttgtcgg gcaaatcggc aatgacgatg gcgtcgcgga
     2461 tgccgaaggt gaagtagaag ctctcgacct tgccgccgag cccgttcacc atattctcga
     2521 ccgtttgttg gcgcgcggtg ccgccgcctt cgacaagccc ggcgttgccg gctgcagagt
     2581 aatcgacttc aagcaggtat ttcggcatgg tgttacctcc gtttaaggca gtccaacata
     2641 ctccaactcg gtcggcgcag gccaggctat aagtcgatga gccgcccgct aaacgcgaca
     2701 tccgggtccg tcctaatagc agttgttcct taccttttgc gttgatgacc gcttcgaacg
     2761 gacaggtggc gttcgctgac ctcatgtggg atctccggaa cgatgatgag tctttgggtt
     2821 tgatggcgct gggctccgca aggacgggat ccttgacctg gaaacgcgcg actgcatcag
     2881 cggcaacttt ctggtctttc ttcagtcatc cggttggcga aaatcttggc agcggaccgc
     2941 aatggtgttc tccaaggtct ggccgtacct ggatggtcgc cttccccaat ccgaccggtt
     3001 gccaccagcg gcgggaatgg ttggatggat cagtgacgac tgctctcggc cccgatccgc
     3061 cacttcgcgg gatctgatga ggctagagct gccagacggc ccccaccgtc cttccggacc
     3121 cggtgtcctc ctctaacctt cgattgacgt actcgatgaa atctggggga aagttgatgc
     3181 ctgtgtcccc cgcggcctcc gacatctgct tccaccaggc gttgcctccc ggtgttgcac
     3241 acgatcctac gtaggcgttg gccaccagat tgaaggcgtc atcattcagg tcggagtctc
     3301 gatacagcag aagcgcgtcg taaaagttgt tggagaagag catcatcaga tgattgaagt
     3361 ggactcgctc atcctgtgtg aggtcgtcga tttggttgaa ggcttttgtg atgaccgctc
     3421 gcgtatccgg ttcgctgacg atatggcgga tggcatccga caagcgatcc agcacgtgtt
     3481 gccgggcgcc aattcgcaga gcctgtgaat tttgcctgac ctgcagtgcg agatagatca
     3541 aggtgacgac tacggcaatc gatccaaaaa actctccgaa atttcccatc agcgatccga
     3601 cccgatcaat atccatggct gaattccctt tgagttgcgt gaatccgaat tctaaagacc
     3661 cgggcggctg tgcgtagtac cggcggtcca tagctgcagg aaccgttcgt gccggagctg
     3721 cgggaggccc tttcggtcga tttccccggc tgataaacgc gcgaacgacg acgcgttagg
     3781 ctgtgcggcc cgccccgcgg gccttgccgg ccagctcggc cagatcgtcg agcatcggca
     3841 acacggtatc ggcgtccgcg tcaggcagcg cgaacaccag ctcgtcgaaa ccctgctcga
     3901 tgcgacccat cagctggtca taatccgtgg gcgccccgaa aagcgccatg tccacgtctt
     3961 ccagcttcct gccgcgggcg gccagcgcct cggaaagccg ttccatgttg cccgaaccga
     4021 taccgccaat gggcatccag ccgtcggcat agtctgcaat acggtcgaag acccatttgg
     4081 agttggcgcc gatccagatc ggcggccccc ctggctgcac cggtttcggg taagaccaga
     4141 tcggatcgaa atccacgaac tccccgtgat actcggcagc ttcgttcgcc cagatctcgc
     4201 gcatggcgag aactttctcc cgaacgatcc tccagcggtt cttatagtcc gcgccgtggt
     4261 tttccatctc ttcccgattc catcccgcgc cgatgccgag caccagacgt ccgttggaca
     4321 gcatatccag cgatgcggcc tctttggcca gggtaatggg gtcacgctcg atgaccagac
     4381 agatcccggt gcccaggcgg atgcggctgg tcacggccgc gcacgcaccg agtgctacga
     4441 acgggtcgtg agtatgccag tacatccggg gcagctcggt gccgcccggg aagggcgttg
     4501 cgcgcgatgc aggaatgtgt gtgtgctccg gaaagaagag gctatcgagt cctcgttcct
     4561 caaccgcttg ggccaacgcc gcgggttgaa tggtgtaatc ggttggaaag atggtgacac
     4621 cgaaattcgg ttttgatgag aacgccatga tgctgcttgc ccctccagtg aatgccggca
     4681 tcataccccg gcgccgtgcc cgaggagcga ggaatcgtgc cgcaagcggt ccttggtagc
     4741 gcgcaatgag ctcatgaagg agcgttaccg aaaccgattt ccgtctgcag ccgctggggc
     4801 gataatcagt tatcgtgata gccgacagga gcttcccgtg gattggcgga cctggctggg
     4861 cctctctctg acgattgcgt ggctgctgct cggggcgctg tacgtctcgg accagatcgg
     4921 atggagccag tttgcagcgc tcaacgctga cgcgttgggc agctttctcg agggcgcctt
     4981 tgcgccgcta gcctttctct ggctggtgat cggttacttt cttcagcaga aagagctcca
     5041 gcagaacacc cgggccctgg aagcgcaggc tcaggagatc cagcgctcgg cggagcaggc
     5101 ggtgatccag tccgagaaga tggcggaatc cgaaatgcat gcgcggcaac aggcctttct
     5161 ccagatcgcc gagagcgtga aacggcagct tggatccatc gggggcatgc tgttcatctc
     5221 cagccagggc tccgacgcgg aggggaacgt cacggccgag gagttcagcg agctcttctc
     5281 catgaaggcg ggacaggacc cggagatatt ctcgcggcgc cttctggaat cgcacacccg
     5341 ggcgcgaccg gagcgtcaat tggctatatt ctacggaacg cccgttcgag ccaggcactc
     5401 aaacaacttc atctacactt tcgaacgatt gctcgcccga gctgagctgg tcgatacaga
     5461 caacatgatt cgcgactcgt tgctgggcag cgcccacggt tttgtgtatt cccttgccaa
     5521 gcggcaccgc gccgcggccc ccgaagagct cgccgacccc agccaaacgg gcaccgacat
     5581 tcgattctga gccgctgccc tcggagtcat cgccagtggc gcatggatat tcgcgccgga
     5641 atttcaagca ttcatcaacg gcattctggc cgaagccggc gattggtaat ggttttcgga
     5701 acggacggtt agcgtcgctc gatgatgcgc cttgcgttca taacatcggc gtttgaccgg
     5761 attttcggag gcctctcctg atagatctgg gtagttggcc ggcagctttc cttgcggccg
     5821 cgttcgctgg agtggctgcg atcatcgcgt ggttcggggt caggatgacc agaatcgccg
     5881 gagatcttgc gcacaatacc gggctggggg aagcgctcgt gggcgcgctc ttcattggcg
     5941 catcgacgtc gttgtctgga atcaccacgt cggtctcagt ggcagcggcc gggcatgcgg
     6001 agctggcggt aagcaacggt ttggggggca ttgcggctca aacgtccttt ctggcgttgg
     6061 cggatatcgt gtgccgccgc gcaaacctcg agcacgcggc tgcctcggcc gaaaatctgt
     6121 tcatgtcggc gttccttctg acactgcttg gtattcatgc gctcgcgctg gcgatcccgg
     6181 atgtgggtat cttcggcgcg cacccggcga ccgtgattct ggtcatcgcg tatgtgtttg
     6241 gtgtgcacct tctggcgcgc actcacgact tgccgatgtg gcttccgata agaacggcgg
     6301 acacgccgac agaaaccgcc gaccacaacg ttcgccgccg tcaccagcgt cgagcagttc
     6361 tggcttgcca actcgatggt gatgaccggc gtgctgctca tgggtctcat ctatcgtgag
     6421 cgccacggtc cggggaacat cggtatggaa agcgtgatcc tgctgctgct ttatctcgga
     6481 gggctggtca ccctgtccgt ctggccctag cgtcggtgct gagggctccg atcccgtttc
     6541 gcattcgacc ctcgaagttc cagcgcatcg agcgacgggg acgaatgggg cgagcacgcg
     6601 cggtgctgcg gcacgacgtt cgttttgcgt taggatgtgg tcctcacgat taccatcttg
     6661 cgggttatcc gcaatcggcc agcctgatcc gcccgccaac atagagcagg ggctgcgctg
     6721 atcgtgaacg acctgatctt ttagtcaatc caaaaggagc aaccgccatg caaggacccg
     6781 tacgcaccat gaccctggac gaagtccgtt cgttcacggg caaggagatc ggagtttcgg
     6841 actggttcga gctcgatcag agcaagatca atcagttcgc tgatctcacc gaggatcaca
     6901 tgtttctgca cgtcgatccg gaagcagcca aggcgactcc cttcggcagc accatcgccc
     6961 atgggctgtt gaccctgtcg atgatgcctg tgatggccta tcaggcgatg cccggcgtgt
     7021 ccgggacgaa gatgggcgtc aactacggct acaacaaggt ccggttcatg gctccggtca
     7081 agagcggcaa gcgcatccgc gggcacttcg tcgtgcaggg cattgaggac aaaggcgatg
     7141 gccggcagca gatcactcac gacgtgagca tcgagatcga aggcgaatcc aaaccagccc
     7201 tggtggcgga atggatgact atggtgtgga tgtaggttct ccctcctgag ggatcagaag
     7261 gtaaaactca tggccatcct ggaactcgtc acccttccac cggcgttcgg tttgcgcaac
     7321 gtcagtccct tctgcctgaa ggcggagatg ctcctcaaat cgctcgattt gccgttcgcc
     7381 cagtcccaac tgcaggaccc ccgcaaagcg ccgaagggta agctgcctta tctcctctcg
     7441 gacggagtga agatcgcgga ttcggagctg attaccgagc acctggacga gatgacccag
     7501 ggccgcgtct atgcggggct gtcccccgag cagaaagcgc aaggggtggc gctgacccgg
     7561 ctggtggagg accacctcta ctggatcggg gtggccagcc gctggctgga cgacgcctgg
     7621 tggcctcacg tcgtggagaa tttctttgga ttcgtgccgg cgctcatccg gcctttcgct
     7681 gccggaggcg cgcgcaagca ggttcgccag acctatcatc ttcagggtct cggtcggcac
     7741 acgctggaag agcagcgcgg ctttgcgaag cgggacctgc aggccctcga ggatgccgtg
     7801 cctcggaccg gttttctctt cggcgatgcg cccgggatat tcgacttcac ggtggccgga
     7861 atgatggccg gcttctatga caaccagccg cccacctggc tcacggagct ggccctgacc
     7921 tatcctgggc tcaacgaata taccgagcgg gtacaggagg ccatcggcgt gtatggccgc
     7981 catgtggatg cctgagcggg tcgggtgagg gcgggaatca ggatcgcggg gtgaactcag
     8041 cgggcaggtg ccagcaggtg tgcatctctc ccaggcgcag gcaccgcagc tcaggctgag
     8101 cctgggtttg cagacgaatg tccggcaggg tgtccagcag aatgttcgaa gcggtcacga
     8161 tggtgtcgcg ggtcatggcg tagagcgagc ggggtcggtc ctcgggaatg cctgggtacg
     8221 gcgacggtct gccgagcccc aatgccactc ctgcagccag accgtcgctt cggtaatgcc
     8281 cgcgaggttc ccgctggcag aggtctctgc gcgtgatgtc gaactcatcg ggatcggaaa
     8341 atatccacgg atcccgattg gcagcggcgg cggcacagta aaacaggcca ccgatgggca
     8401 gcagccttcc aaacctttcc acctcgtgaa gcgcgaagcg cttcttccac agcacggggg
     8461 tggagtggcg cagggcctcg aggtaggcga acttcagcag ccgacgttcc tgtgcaacct
     8521 gttcgagctg gtcggggtgg gtgaggagca gaaaccagaa gttggcaagc gccccgtgca
     8581 tggtctcgtg gtcggcttcc aggagagtca ccacgacgtc ttctgctgta gtagggccgt
     8641 cttcgagctc cagggtagcg atcgccgaga ccatgtcatc ccccggatcg gcacggcgcg
     8701 cctcgagtaa gggcctgaag tagtctgcca gctcggtcat cgcccgcttg cccgcctgct
     8761 cgtctgcggc gttccagtcg aatccgcgct gcatgcgcca gtagcgctcc acgaaccagt
     8821 gaaagtcctc cgccggaaga tccagcactt ttgccagcag ttccatgggc aggcgggcgg
     8881 cgaactccag agcaagatcc gcggagcccc tggctgcaaa agcagacacg atctcctcgg
     8941 ctaccggcac tgcgttggcg tcgatgagcc gctcgtgcgc gatctgcatg ggcagctcag
     9001 aacgcaggtc gcgtccgtag ccctgcatac gatagaacca tagcttcgga cgggtttcga
     9061 agttggcatc gtcggtaaag acggacgtca cctggttgta gcgcgtaagc cagtaggcgt
     9121 tgcccagcca gtcccgatag cagggatagt tttcccgaag cacgccgaga acggggtagg
     9181 ggtcccggac gaactctgac gagacgagct cgatgggacg aatcttctcg gtggattcgt
     9241 gcacacgctc gcgctgatag atctcgtagg tacggtaatc ggcagccgtg ccgttgcgcg
     9301 caaacagcgg catgccgctg cgcgacaggc tcggccgcga cttgcccagg atcctcacag
     9361 gatccccttt gcttgctttg acgaactcgg actcgctgct catggcttag ggctggtcga
     9421 tgtcgtagtc gatccacaga tcgcgcagtt tgttgattcc gatcggcgcg aggctcacgc
     9481 cgtctatgtc cttcggcatc ctctcgcggt tgatgcgagc gttcttcaga cgctttgcca
     9541 gcaggcgcga tccgatggtc gcctcctgat ggctgatcca ggcgccagga cacatgtgtg
     9601 gccccacgcc gaaggccatg tggctgcaga cgccgtcttt gctgtagccg ctgcgcagca
     9661 gcttcccgga gtacagatcg tcgcggaaga tatcgaaagt ttccggatcc ttgaacaccc
     9721 gctcgtcgtg gctgcccgag aagttcacca tctcgatgag cgagcccgca gggatgtgca
     9781 cgccgtgcag ctccacgtcg aaggtggcgt ggcgcggttg cccgccgatc gggttggaat
     9841 ggcgcagggt ttcgtggaag gcggcgtccc agagagcgtc gtcggcaact acggcggcga
     9901 actgctcggg atgctgcagc agcgaatacc acatgttcat gatggtgccg cgggtcgtct
     9961 cgcccccgcc ggccaccacc agagcgatgt acgaggtgat ctccacggag ctgaggtagt
    10021 cgccgtctat cttgctcgcg caaagcttgg aaatgatgtc cggacccagt ttgttgccct
    10081 ggttgtcgta aagatatccg gggctcctgc gtcgctcctc cacgagctcg gacacgtagt
    10141 cctccaggtg ctggcgggcc tccatgccct gcttgtgggt ctcagacgcc ccgagtccgg
    10201 acatgatgga gtcgtaccag tagaagaagt gggcctgcgc ctccttggga aaccctagca
    10261 catcgcacac cactcggacg ggaaattcgt aggcaaaagc gcgacccaac tcgatggtgg
    10321 caaccttgcc gctcacctgg cggacgcttt cgtcgagatt cgcgaaatcg tcgatcatct
    10381 cgagcgccag ccgctcgatt gcgggcaccc gatgttccag ggatttcccc accaggtgct
    10441 gcccgaacag attgcgccgg cgacggtgct cgacgccgct gaactcgtgg gtgacgttgc
    10501 ccagcacgcc gttggatgag ccctttggaa tggtgttgaa gccgatgtcg tcgtagtagc
    10561 actcggtcat gtccgcgtat cgcgtcacgt agtacgtatt gttcagcttg ttgaagtaca
    10621 ccgggtagtg gtcgcgcagg atccggtagt agggccaggg gtttcgacgg aactctgccg
    10681 agtagaagat gcgcggatcg atcagggggg tgccatcctc agcgcgcccc atgtcggcgg
    10741 cctctcccag cggcatcagg ggaaagtcga tcttggcgcc cgggtcggcg tcgccggcaa
    10801 tcgtctcggc gccgatggtg cggcgaagag tgtcggcaaa tgcgctctca gtggacatgt
    10861 gctctgtatc ctttcatccc gaccctgatc ctagggcttt tttccgggtt tctccaagca
    10921 caaactgttt gtgcgtcgtt tcgattggcg ttctccagaa aggggtgacc agaccgattc
    10981 ggcgagcgct cagggcttaa agggctttgc tgaccgcatc ggaggccatt tttccggttc
    11041 gaccacggcg ttggttgcag gcaacaggac agaaaacacg gctcgatacc cgagggcgtc
    11101 gctcattact catcaacggc tgacgacaaa ctgcaagatt aggataggct ttcgggaatc
    11161 aacgggaggg taaagaaagt ggatctgaac gctactgtgc agctattggg gaatctcggg
    11221 gagtttgtcg cggcgatcgc cgtggtggcg tccctgctgt tcgttggctt ccaggtcaga
    11281 caaagtgacc gcacgcaacg cacgaccagc ctgcagtcgg tgctcgatgg acaccgggat
    11341 cgcacgtttg cgccgttata cgagagcgag gacatggcag atctgtgggc tcggggtctc
    11401 acgtcgttcg acgacctgag cgaaaccgag aagcgacgct ttttcagctt catgttcgag
    11461 caatgctttc agatgcagca ggtaatgcag ttgcatgaac agggtctggt ccccgacgtc
    11521 gatttcgatg cgtggcttta ctacacgggc gtgctgttca attgtcccgg gggtagggcg
    11581 gtgtggccga tcatagagag gttggtcact ccgacggtcc gggacctgat gaacgaatat
    11641 tgggatgaga acccgacgtt gcctacgtac caggagctca atccgctctt cgtcgcggac
    11701 cgggcagccg gggtgatagg cacatagagc cgcgaaccgt ccttccattc cttgtgcgcg
    11761 cgggctgttt ccagttggct gcgcagaaag tatcgttgac ggatcgtgat ctttcctcaa
    11821 tcggccggct ggtgagctat cgttatgcct tcaatcccct cgaacatcga agaagttacc
    11881 aacgaatgga tgtcggagat cctcggtgcg gaggtcaccg ggttcgatgt gacattcctt
    11941 gaaggtggcg tgctgtctga tgcgttcaag gttcacgaca tcgcctacgg tgcgcctgcg
    12001 ggagatcagc ctgcgtcggt cgtcgtgaag ctgaccaatg cggtcccgga acggcgtgcg
    12061 gtcgccgcgc agaacaaggc ttacgtcaag gaggttcgct tcttcaagga gttggcagat
    12121 caggtccc
//
LOCUS       DBNHLM010000164         5422 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_23781, whole genome shotgun sequence.
ACCESSION   DBNHLM010000164 DBNHLM010000000
VERSION     DBNHLM010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_23781"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="AC16QF_07485"
     CDS             complement(<1..943)
                     /locus_tag="AC16QF_07485"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF040257.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent amine dehydrogenase family
                     protein"
                     /protein_id="HIR6448436.1"
                     /translation="MALKVFQWASGTVGRHAALAVLERDGLELVGLHTLSAAKAGRDV
                     GELLGVAATGLRATSELEAVLESDADVVVHAPLASLVYGEDPGRDVEDICRLLASGKN
                     VITVVGYMYPKVYGPELVARLEDACRAGKSTFHSTGLNPGWMGDLVPLTMSALSRTID
                     RVHVLEISNFQGYPSPEIMFESMGFGSSPEEFERSGERRRNWLNGLFRESVQMVADGL
                     GLELDDVVEEMTLEFAPRDLETAAGTVRQGTVAGQHWEWAGVLGGDKRVIHETVWRMH
                     ADVAPDWPQGRHAVTVHGVPRIHLEFEADYVSDGLLAT"
     gene            complement(967..2076)
                     /locus_tag="AC16QF_07490"
     CDS             complement(967..2076)
                     /locus_tag="AC16QF_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008023997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR6448437.1"
                     /translation="MSTTTALAAVAWAPDDPFAFEEVELGPLGADEVLVRIVASGICH
                     TDVAVKEQSVKLPLPMVLGHEGSGIVEAVGSAVSHLMPGDHVVLSSDSCGVCRRCHAG
                     LPSYCDEFVERNLTGFRTDGTSPLACRGEPLRGRFCGQSSFATAAISPGRSAVKVPED
                     LPLELLGPLGCGLTTGVGTVMNALNPGPGSSIAVFGVGTVGLSAVMGAVLSGCEAIIA
                     VDPNLARLEVARELGATECIDAGREDVAADIVELTRGGADFSVECSGHPAAIDQAVAC
                     LGRPGWCAQVGAPTAGTRFPLDMDHVGFGRGIRGVVLGEANVHTFVPYLASLYRDGRL
                     PYDRLVRYYDFAEIDRAVHDSAVTGEVIKPILRMS"
     gene            complement(2073..3176)
                     /locus_tag="AC16QF_07495"
     CDS             complement(2073..3176)
                     /locus_tag="AC16QF_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6448438.1"
                     /translation="MEMRVLGRTGVSVSKLCFGAGMFGYFGNPDERECARMVDAAIDA
                     GVNYFDTSDVYSHGESESILGKALVGKRDDVILATKFSLPMSDDPNHRGNSRRWIARE
                     VEASLKRLQTDYIDLYQVHRPDVTADIDETLGALSDLVRSGKVRMLGSSTFPAEQIVE
                     AQWVAEKRHRERFVVEQPPYSIFARRIEADILPTCQRYGMGVVAWSPLSQGWLTGKWR
                     RDAPPNDTNRQKLQPHLYDMARADNQRKLDLVEQLAAVASEAGISLMHMAIAFVCAHP
                     AVTSAIIGPRTPEQLQGLLDGADVTLGDDVLDAIDAIVAPGTTLSRDDDGYVAPEIAN
                     KALRRRFVAEEVHEAARETLRNIEAYREQQGKK"
     gene            complement(3182..3703)
                     /locus_tag="AC16QF_07500"
     CDS             complement(3182..3703)
                     /locus_tag="AC16QF_07500"
                     /inference="COORDINATES: protein motif:HMM:NF025050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6448439.1"
                     /translation="MSIIRHPIWQYAYFVNDIDEACHKWNRMVGAGPFHVVRHHIAEG
                     FLYRGEHVEADVSYAFGQAGPAHIQFIAQHDDTPSIYRDMYAPGEQGFHHIGLLVHDV
                     DGEIKRFNEAGYETACTLWGGDYVAYMDCRQDMGCYVELHGDAPIIRDLFDGWKRQHE
                     EWDGVTDLIREQG"
     gene            3819..3998
                     /locus_tag="AC16QF_07505"
     CDS             3819..3998
                     /locus_tag="AC16QF_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448440.1"
                     /translation="MSVQNGERLIRFLRSDPQLREKVKDAGWEKFQDVTAEAGASCTT
                     YEVVAALIREIENDS"
     gene            4015..4356
                     /locus_tag="AC16QF_07510"
     CDS             4015..4356
                     /locus_tag="AC16QF_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448441.1"
                     /translation="MGNKLILVGLVEPQTDDLVEAFNQWYLGNHVEDTYNCPNITSVR
                     CFKAVRGFLGEPPSGYLTIYEFEGEDAEEAERILGQYQSDPDAWPKREPNNGSMKVVG
                     AGWYAEEIAFG"
     gene            4380..5207
                     /locus_tag="AC16QF_07515"
     CDS             4380..5207
                     /locus_tag="AC16QF_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6448442.1"
                     /translation="MPHVRINGARLWYEVRGSGEPLLLHHGYTASRVNWLPVAERLQD
                     RYQVVLMECRGTGDSEHTAEGYSLAQYAADVVGMLDHLDLERVTFAGHSMGGGIGYLL
                     GLDHAHRLDRLVLMAPIPAAGIAGLDPDARQKRLKLRRDQNRDALLAEYRAARFRPDV
                     ETDDWIESRVDHVLGVSDGHYEASAESMVELDVGHRLPELEVPTLMLAGAADELLRAN
                     LHDFLALPNATLEVLSRAGHEVAVHEPDAVARAIDAFMTHGPVTARHIRQQMEGESR"
     gene            5204..>5422
                     /locus_tag="AC16QF_07520"
     CDS             5204..>5422
                     /locus_tag="AC16QF_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6448443.1"
                     /translation="MSAPPTRRRWQVADAFGDGNIEIAGLDWGGSGPLALLHHANGFC
                     AATWTLVARGLAGSHRVIAIDARGHGDSG"
BASE COUNT          992 a         1825 c         1745 g          860 t
ORIGIN      
        1 cggtggcgag cagaccgtcg ctgacgtagt cggcttcgaa ctccagatga atgcgcggca
       61 cgccgtgcac ggtgacggca tggcggccct gaggccagtc gggcgcgacg tcggcgtgca
      121 tgcgccagac agtttcgtga atcacccgct tatcgccccc gagcacgccg gcccattccc
      181 aatgctggcc ggccacggtt ccctggcgga cagtcccggc tgcggtttcg aggtcgcggg
      241 gagcaaactc gagggtcatc tcttccacca cgtcgtcgag ctcgagccca agaccgtcgg
      301 ccaccatctg cacgctttcc cggaagaggc cgttgagcca gttgcggcgc cgctccccgg
      361 atcgctcgaa ctcttcgggt gacgatccga accccatgga ctcgaacatg atctccggcg
      421 acgggtaacc ctgaaagttg gagatctcca ggacgtggac gcgatcgatc gtccgcgaca
      481 gcgcgctcat ggtcaacggc accaggtctc ccatccagcc gggattcaag cccgtggagt
      541 ggaaggtgga cttacccgcg cggcaggcat cctccagcct cgcgaccagc tccggcccgt
      601 agaccttcgg atacatgtag cccaccacgg taatcacgtt cttgcccgag gcgagcagcc
      661 tgcagatgtc ctcgacgtca cgacccggat cctcgccgta aacgagcgag gccagcggcg
      721 cgtgaacgac cacgtcggcg tcggactcca gcacggcttc gagttcgctg gttgcccgca
      781 ggccggtggc ggcaacgccc agcagctcac ccacatcccg gcccgccttg gcagcggaca
      841 gggtgtgcag gccaacgagc tccaggccgt cacgttcgag cacggccagg gcagcgtgac
      901 gcccgacggt gccgctggcc cattgaaaaa ccttcagggc catgggatcc cctccaaata
      961 cctgcgctag gacattctca gtatgggctt gatcacctca ccggtgaccg cgctgtcgtg
     1021 aacggcacgg tcgatctcgg cgaaatcgta gtatcgcacc agccgatcat agggcagccg
     1081 cccgtcgcgg tagagcgaag cgaggtaggg cacgaaagtg tgcacgttgg cctcgccgag
     1141 gaccacgccg cgaatgcccc tgccgaaccc cacatgatcc atgtccagag ggaaacgggt
     1201 tcccgcggtt ggcgcgccca cctgagcgca ccagcccgga cgcccgagac acgccaccgc
     1261 ctgatcaatg gccgcagggt gacccgagca ctccacggag aaatcggcgc cgccccgggt
     1321 cagctctacg atatctgcgg cgacatcctc gcgccccgcg tcgatgcact ccgtggcgcc
     1381 gagctcgcgg gccacctcca gccgcgccag gttgggatcc acggcgatga tcgcctcgca
     1441 gccggagagc accgcgccca tgacggcgga aaggcccacc gtgccgacgc cgaatacggc
     1501 gatgctcgaa cccggacccg ggttcagagc attcatcacg gtgccgaccc ccgtggtaag
     1561 accgcagccg agcgggccga ggagctccag gggaagatcc tcagggacct tcaccgcgct
     1621 gcgtccgggc gagatagcgg cggtcgcaaa cgaagattgg ccgcagaagc gtccgcgcag
     1681 aggctcgccc cggcaggcca gcggactcgt tccatccgtg cgaaagccgg tgagattgcg
     1741 ctccacgaac tcgtcgcagt aggagggaag cccggcgtgg cagcgccgac agacgccaca
     1801 tgagtcactg ctgaggacca cgtgatcgcc cggcatgagg tgcgagaccg cgctgccaac
     1861 ggcctcgacg atcccggatc cttcgtggcc gagaaccatg ggcaacggca gcttgaccga
     1921 ctgctccttg acggcaacgt cggtgtggca gatgcccgag gctacgatcc ggaccagcac
     1981 ctcgtccgcg cccagcggcc ctagctccac ctcctcgaag gcaaacgggt cgtccggcgc
     2041 ccaggcgacg gctgcaagag cggtggtcgt gctcatttct ttccctgctg ttcgcggtac
     2101 gcctcgatgt tcctgagggt ttcccgcgcg gcctcgtgaa cctcctcggc cacgaaccgg
     2161 cggcgcaggg ccttgttggc gatctccggg gccacatagc cgtcgtcgtc gcggctgagg
     2221 gtagtccccg gagcgacgat ggcatctatg gcatcgagca cgtcgtcgcc gagggtcacg
     2281 tcggcgccat cgagcagccc ctgaagctgc tcgggcgtgc gcgggccgat gatcgccgag
     2341 gtcaccgcgg gatgggcgca gacgaaggcg atggccatgt gcatcaggct gatgcccgct
     2401 tcgctcgcaa ccgccgccag ttgctccacc aggtcgagct tgcgctgatt gtcggctcgg
     2461 gccatgtcgt acaggtgcgg ctgcagcttc tgccggttgg tgtcgttggg cggcgcgtcg
     2521 cggcgccact tgcccgtgag ccagccttgc gacaacgggc tccacgcaac aacccccatg
     2581 ccgtaacgct gacaggtggg caggatgtcc gcttcgatgc gccgggcgaa gatcgagtag
     2641 ggcggctgct cgacgacgaa acgctcgcgg tggcgtttct cggcaaccca ctgggcttcc
     2701 acgatctgct ctgcggggaa cgtggacgag cccagcatgc ggaccttgcc cgagcgaacc
     2761 agatccgaaa gggcgcccag agtctcgtcg atgtcggcgg tcacgtccgg ccgatgaacc
     2821 tgatagagat cgatgtaatc cgtctgcaga cgtttcaggc tcgcctcgac ctcgcgcgcg
     2881 atccagcgcc gcgagttgcc gcgatggttc gggtcgtcgc tcatcggcag gctgaacttg
     2941 gtggcgagaa tcacgtcgtc gcgcttgccg acgagcgcct tgccgagaat ggattccgac
     3001 tccccatggg agtacacgtc ggacgtgtcg aagtagttga cgcctgcgtc gatggcggca
     3061 tccaccatgc gcgcgcattc gcgctcgtcg ggattgccga aatagccgaa catgcccgca
     3121 ccgaagcaga gcttgctgac actgacgccc gtacggccga gaacccgcat ctccatgacg
     3181 ctcagccctg ctcgcgaatc agatccgtga cgccgtccca ctcctcgtgc tggcgcttcc
     3241 agccatcgaa gagatcgcga atgatcggcg catcaccgtg cagctccacg tagcaaccca
     3301 tgtcctgacg gcaatccata taggccacgt agtcgccgcc ccagagggtg caggcggtct
     3361 cgtagcccgc ctcgttgaac cgcttgatct ccccgtcgac gtcgtgcacg agcagcccga
     3421 tgtggtgaaa gccctgctcg ccgggcgcgt acatgtcccg gtagatcgag ggcgtgtcgt
     3481 cgtgctgagc aatgaactga atgtgtgcag ggccggcctg gccgaacgcg tagctcacgt
     3541 ccgcctcgac gtgctcgccg cgatacagga aaccttcggc gatatgatgc cgcacgacgt
     3601 ggaacggtcc cgcgcccacc atgcgattcc acttgtgaca ggcttcatcg atgtcgttga
     3661 cgaagtaagc gtactgccag atgggatgcc ggataatgct catgatgtct cccctttgaa
     3721 atccacctca aaccttgccg aacgatagca gactaacccg acgccgccga gtgctgtaga
     3781 ctcggctacg aaaccgacat ctcgaaaagg ggggggacat gtccgtacag aacggcgagc
     3841 ggctcattcg tttccttaga agcgacccgc agctgcggga aaaggtcaag gacgctggat
     3901 gggaaaagtt tcaggacgtc actgccgagg ccggtgcctc ctgcacgacc tacgaggtcg
     3961 tcgccgcgct cattcgcgag atcgagaacg acagctgaac aggaaagacg attcatgggc
     4021 aacaaactga tcctcgtggg cctggtagag ccgcagacgg atgacctggt cgaggccttc
     4081 aatcagtggt acctgggcaa ccacgtggaa gacacctata actgcccgaa catcacgtcg
     4141 gtgcgctgct tcaaggccgt gcgggggttc ctgggcgagc cgccctccgg gtacctgacc
     4201 atctacgagt tcgaaggcga ggacgccgag gaggcggaac gcattctcgg tcagtatcag
     4261 tccgatcccg acgcctggcc caagcgcgag ccgaacaacg gatcgatgaa ggtcgtgggc
     4321 gcaggctggt acgcagagga gatcgccttc ggctgagccc gagcgcggcg accgtaaaaa
     4381 tgccccatgt ccgcatcaac ggggcccgtc tgtggtacga ggtccgaggc tccggcgagc
     4441 ccctgctgct gcatcatggc tacacggcgt cacgggtcaa ctggctgccc gtggccgagc
     4501 gtctgcagga ccgctatcag gtcgttctca tggagtgccg gggtaccgga gatagcgagc
     4561 acaccgctga gggatacagc ctcgcccagt atgcggccga cgtcgtcggc atgctggacc
     4621 acctcgacct ggaacgggtg acctttgccg gccacagcat gggcggcggc atcggctacc
     4681 tgctgggcct cgatcacgct caccggctgg atcgcctggt gctcatggcg cccattcccg
     4741 ccgcgggcat cgccggcctc gatccggacg cgcggcagaa gcgcctgaag ctgcgccgcg
     4801 accagaaccg cgatgccctg ctggcagagt accgggccgc ccgctttcgc ccggacgtgg
     4861 aaaccgacga ctggatcgag agccgcgtgg accacgtgct cggcgtcagc gacggacact
     4921 acgaggcgag cgcggagtcc atggtcgagc tggacgtggg ccaccgactg ccagagctgg
     4981 aggtacccac cctcatgctc gccggcgcag ccgacgagct gctgcgcgca aaccttcacg
     5041 acttcctcgc cctgcccaac gccaccctgg aggtgctctc cagagcaggc cacgaggtgg
     5101 ccgtgcacga gccggacgcc gtggcccggg ccatcgacgc cttcatgacc cacggccccg
     5161 tcacggcccg gcacatcagg cagcaaatgg agggggaatc cagatgagcg cccctcccac
     5221 ccgtcgcaga tggcaggtag ccgacgcctt cggcgacggg aacatcgaga tcgccggtct
     5281 ggactgggga ggcagcgggc cgctggcgct gctgcatcac gccaacgggt tctgcgccgc
     5341 aacctggacg ctcgttgcca gaggccttgc cggcagccat cgggtcatcg ccatcgatgc
     5401 ccggggccac ggcgactcgg ga
//
LOCUS       DBNHLM010000165         6140 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_262688, whole genome shotgun sequence.
ACCESSION   DBNHLM010000165 DBNHLM010000000
VERSION     DBNHLM010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_262688"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="AC16QF_07525"
     CDS             <1..264
                     /locus_tag="AC16QF_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin containing oxidoreductase"
                     /protein_id="HIR6448444.1"
                     /translation="AGDRSIAEVAVSVDFGATWQPAVLARPANRLAWQRWRATVKLPV
                     PGYYEIWARAIDSAGVSQPMLVPGWNPKGYLNNAAHRIALRAG"
     gene            264..620
                     /locus_tag="AC16QF_07530"
     CDS             264..620
                     /locus_tag="AC16QF_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448445.1"
                     /translation="MAKAFCIWLSGLVVLTAASVGAAEEDAATGLAVAPGWDSVRTHC
                     GACHSYRLVTSQRGDEAFWRDIIRSMQGTQNLWPIAAEEEARIVGYLAANYAETDWGR
                     RPPLSPLLMPGDPTPD"
     gene            699..896
                     /locus_tag="AC16QF_07535"
     CDS             699..896
                     /locus_tag="AC16QF_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448446.1"
                     /translation="MALQDLVGKCFSVAEGEYRIVDVRNIAGDALVYAEKRQGLKEFM
                     PSFPKRAAFHYGDIARYLAQS"
     gene            1333..2082
                     /locus_tag="AC16QF_07540"
     CDS             1333..2082
                     /locus_tag="AC16QF_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448447.1"
                     /translation="MDELVLLFTADGGKVVSEIRYGEFEALIKKETSLAEHAASVVKA
                     AYALVGGGLTLRGIVFFRFTVDEDGKIVGSFNLPLRYLLDNAGLGPDLGAGPIQLACR
                     GQCPVPWHSVNLWQPEGEGDGHPAMLAQKAIWRNRTGLKRTAAPPMAQPLRSQPVEPP
                     VIDEQRHLEARLTAAFGEEGKVNLTQLVQQHSAQLSEVGQQHRLDLEEQHRTFQDQLR
                     EARDEIHKLKSALRREQERNRRLQSLLRGDP"
     gene            complement(2124..2567)
                     /locus_tag="AC16QF_07545"
     CDS             complement(2124..2567)
                     /locus_tag="AC16QF_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448448.1"
                     /translation="MSEPTPIRPQPSDADLPALYPLTGVVLATVLGSFIAAVVVVYLN
                     YRSLGSTALARKAVIGGLVVYAGLLGLTVVLPRETYMSIIFIGVQAGLAYVVANALQG
                     DAIAYHVSRGGPIHSLLRAAGVGLLTGLAVLFALLTATALILGTS"
     gene            2664..3326
                     /locus_tag="AC16QF_07550"
     CDS             2664..3326
                     /locus_tag="AC16QF_07550"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HIR6448449.1"
                     /translation="MLIEIFSDVVCPWCYIGKRRLDMVMQTEVGEGVELAWRPYQLYP
                     NIPAGGLLREDVLRARHPEADDEDLETLKRKVPSRIRAEAEGVGIEFDFAAMATVPNT
                     RLAHRLLMFVEDVVGSLALQHDLAEELFSLHFCHGQDVGDAEVLVEAARRLGLDVDAV
                     RAYLAGDSGSAALDRELARAVDVGVSGVPGFLLGGRFLLPGAQTPEVIGQFISRAKER
                     LG"
     gene            3510..>6140
                     /locus_tag="AC16QF_07555"
     CDS             3510..>6140
                     /locus_tag="AC16QF_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6448450.1"
                     /translation="MRASNVFLGGFALGYAVVAASSAWAEEVIEEVVVLGSYIKRAPE
                     DAPSPVRVIGREEMEAAGRPQLADYVMTLPSVVGSENVTAQEQNVAGAGAANINIRNL
                     GLSSTLVLINGKRLNVGTSVSNQGENFVDINRLPSIMIENVEILKDGASATYGSDAVA
                     GVANFKLRDRFEGFEIQGFYQDSFKGNEVSFNKVGLPEIYRPVIEARSKDTYSDLDLG
                     AIWGFGNDKTHFVIGANYFKRDPLESVDRQFAINDIADGSVGGPSPFNLPQDMFGSVI
                     LAPGLEIPGAALINDSSCVALGHYRTRNSGLCSQKLDLLSRDIFSKEERRQLLATFTH
                     DLSDRVEIYGHFGGSETDVRINQSPSFPITSQVPFSSANPGFQFEVLNGFQSLLTTGT
                     APFGDDVNNRLASLLAGQPYPLPVLPFVPPELGGPNPLDPFAILSQVGTLTFNGVMRP
                     SLLHLAQIAGVPADVDGDGVVEEGEIFRNRNQSTIDRETRVFMIGARGDLNDAWSFDT
                     SFNYSSEKSITVFYDTVTDRLSDALNGWFGLGCDKDAYGPGEGPCTWFNPFGSSMLLP
                     DVVFPDGNGNLHTLGNDAAAVHALEGEGVATARSSLIVVDAIMSTNEFFGWELGGGGV
                     GFAIGAQYRKEEYKAGGNELATDPSFPFAFTGPTIPFDADQDIWAIFTEFALPLTEDL
                     EVQLALRYEDYGDDTGDTIDPKVAVRWQAADSLVLRASAGTSFRGPSLVQKFGTGTGL
                     QFILPPSPEVIGANVPGQTFGSGVFARLPTYGNPGLQPEESVNFNIGVIWSPNENLSI
                     SLDYFNYDYEDVIVPDDFRGLANDCQINWGLAGRPVPQNVDGSFNADYLAVEACNFLD
                     LDGDPGTPDIFLDTQGNPLTV"
BASE COUNT         1150 a         1809 c         1958 g         1223 t
ORIGIN      
        1 gccggcgatc ggtcaatagc ggaggttgcg gtgagcgttg atttcggtgc gacctggcag
       61 cctgcggtat tggcgcgccc ggcaaaccgg ctcgcgtggc agcgatggcg ggccacggta
      121 aagctccccg tgcccggcta ctacgagatc tgggcccgcg cgatcgattc ggccggcgtg
      181 tcccagccca tgctcgtccc aggatggaat cccaaggggt acctcaataa cgccgcccac
      241 cgaatcgccc tcagggccgg ttgatggcga aggccttttg catctggctc tcgggtctgg
      301 tggttctgac agccgcatcc gtgggagccg ccgaagagga cgcggcgacg gggttggcgg
      361 tggcgccggg gtgggattcg gtgcgcactc actgcggtgc ctgtcactcg tatcgattgg
      421 tgacctcaca gcgcggtgac gaggctttct ggcgggacat catccgctcg atgcagggga
      481 cccagaatct ctggcccatt gccgccgaag aggaagctcg catcgtcgga tacctcgcgg
      541 ccaactatgc cgagaccgac tggggtcgcc gcccgccgct ttcgcccctg ctcatgcccg
      601 gcgatccgac gccggactaa aacacccatt acaacccctt acccctgcac cggggacctt
      661 tgcctatgct gaaactctgc gtgtatgagg tgttacgcat ggctcttcag gatctggttg
      721 gaaagtgttt ttcggtggcc gaaggcgaat atcgcatcgt ggacgttcgc aacattgcgg
      781 gggacgcgct tgtgtatgcc gagaagcgtc aggggctcaa ggagttcatg ccgtcgttcc
      841 cgaagcgtgc ggcatttcac tacggtgata tcgcaagata tcttgctcaa agctgagccc
      901 gccagccgcg atcgtttcac gttaggttca tcgactcggt ctaggatctg ggttcctgga
      961 ggtctttgtc acatggaact tgaagagatc ggcgagcccg agctcgagaa tcactggatc
     1021 gacgagtcgt cggctgacga cggcgaatcg gtctgggtgg atgcctacga gctctagtca
     1081 agtagccgtc tgtttcttcg ccgccacccg catattcgct gcgctcgtcg cccgccgaat
     1141 tcagcggaaa gccgtgatcc cattggcagt atggggaatg gccatctgct gtaatgaggc
     1201 gagtcgcggt gtttgtgtcc cgtgcgagct tcatggaaga gtgcttctgg tctgcggatg
     1261 gccactttcg atagggatgg gctgttccaa ccgggcgcgt tgacaagctt ggaaatgtca
     1321 gtagatcagg atttggatga actcgttctc ctgttcacgg cagacggcgg caaagtcgtc
     1381 tccgagatcc gctatggaga attcgaggct ctcatcaaaa aagagacgag cctggccgag
     1441 catgcggcca gcgtggtgaa ggctgcctac gccctggtag gcgggggtct gacgttgcgc
     1501 ggtatcgtct tctttcgctt caccgtcgat gaggacggca agatcgtggg gtctttcaac
     1561 ctgccgttgc gttatctcct ggacaacgcc gggctcggtc ccgatctcgg agcagggccg
     1621 attcaactgg cctgtcgggg gcagtgtccg gttccatggc actccgtcaa tctctggcag
     1681 ccggaggggg aaggagacgg ccatccggcg atgctcgccc agaaggcgat ctggagaaac
     1741 cggaccggtc tgaaacgtac ggcagcgccc cccatggccc aaccgctgcg aagccagcct
     1801 gtggaacccc cggtcatcga cgagcaacgg catctcgaag ctcggctgac cgccgccttc
     1861 ggtgaggaag gcaaggtcaa tctcacccag ctggttcagc agcacagcgc ccagctttcg
     1921 gaagttggcc agcagcatcg gttggatctc gaagagcagc atcggacctt tcaggatcag
     1981 cttcgcgagg cccgtgacga gatccacaag ctcaaatcag cactcaggcg tgagcaggaa
     2041 cgcaatcgcc gtcttcagtc gctgctgcgc ggggatccct gacccggttc gggccatctg
     2101 ccgcctgctg cttttcagcg gccctaagac gttcccagga tcagcgcggt cgccgtcagc
     2161 agagcgaaca gtaccgcaag ccctgtcaga agcccgacgc cggcggcgcg cagtaacgaa
     2221 tggatgggtc cgccccgtga aacgtggtag gcaatggcgt ctccctgcag ggcgttggcg
     2281 acaacgtatg ccagccccgc ctgcacgccg atgaagatga tgctcatgta ggtttctctc
     2341 ggcagcacca cggtgagtcc cagtagaccc gcgtagacca ccagcccacc gatcaccgcc
     2401 ttgcgtgcga gagcggttga acccagcgac cggtagttca ggtagacgac cacgacggcg
     2461 gcgatgaacg aacccaggac tgtggcaagc accacgccgg tgagcgggta gagcgccgga
     2521 aggtcggcat cagaaggctg cggccggatg ggcgtgggtt cggacatgct gcggagttta
     2581 aagtctgacc gtctgttcta ccaggaacgg gcccgccacc cttacaatga atcgatcacc
     2641 taccggtgca tgcgaggccg gcaatgctga tcgaaatctt ctccgacgtg gtctgtccct
     2701 ggtgctatat cggcaagcga cgattggaca tggtcatgca aaccgaggtc ggtgagggcg
     2761 tcgagcttgc atggcggccc tatcagctct atccgaacat ccccgctggc gggctgctgc
     2821 gcgaagacgt gctcagagcc cgtcatccgg aggcggatga cgaggatctc gagacgctca
     2881 agcgcaaagt tccctcccgt attcgtgcag aggcggaagg cgtcggaatc gaattcgact
     2941 ttgccgcgat ggcaaccgtt ccgaacacgc gcctggccca caggctgctg atgttcgtcg
     3001 aggacgtcgt cggctcattg gcgctgcagc acgacctggc cgaggagctc ttctcgcttc
     3061 atttctgtca cgggcaagac gtgggcgatg ccgaggtgct ggtggaagcg gcgcggcggc
     3121 tgggtctgga tgtcgatgcg gtgcgcgcct atctagcagg ggattccgga tccgccgcgc
     3181 tggatcgaga gctggcccgc gcggtggacg tgggcgtgag cggcgtgcct ggttttcttc
     3241 tcggtggccg cttcctgttg ccgggcgcgc agacgccaga ggtgatcggc cagttcatct
     3301 cccgtgccaa ggagcgcctt ggctgaatcg ccgatttgcc ttggttaggc cgatcgatct
     3361 ccagcctgcc caaccgcacg tgggcggccg tgggaattga acgagcggta gtatcgaaat
     3421 gctgcagtat ttgataagtt cggacgatct gatacgaaat ttgacattgg gagagtggcc
     3481 ggtccgagat atttaacgag gggaatacga tgagagcaag caatgttttc ttgggagggt
     3541 tcgccttggg ctacgcggtg gtggccgcgt ccagtgcctg ggcggaggaa gtgatcgaag
     3601 aggtggtcgt cctcggctcg tacataaagc gggcacccga ggatgcacct tcgccggtcc
     3661 gggtaatcgg gcgtgaggag atggaggccg cgggacggcc gcagctcgca gactacgtca
     3721 tgacgctccc atcggtggtc ggatccgaga acgtcacggc tcaggagcag aacgtcgctg
     3781 gtgcgggcgc ggccaacatc aacatccgca acctgggact gtcatctacc cttgtgctga
     3841 tcaacggtaa acgcctgaac gtggggactt cagtgtccaa tcagggggag aacttcgtcg
     3901 acatcaatcg tcttccgtca atcatgattg agaacgtaga gattttgaag gacggcgcct
     3961 cggcaaccta cggctcggat gctgtggcag gggtagccaa tttcaagctt cgggatcgct
     4021 tcgagggctt tgagatccag ggcttctatc aggattcctt caagggcaac gaagtgagct
     4081 tcaacaaggt cgggctgccg gagatttacc gcccggtgat cgaggcgcgc tcgaaagaca
     4141 cgtattctga ccttgatctt ggcgccatct ggggctttgg caacgacaaa acccacttcg
     4201 tgattggcgc caactacttc aagcgggatc ccctggagtc ggtcgaccgt cagtttgcca
     4261 tcaacgacat cgcagacggc agcgtcgggg ggccgagccc tttcaacctg ccccaggaca
     4321 tgttcgggtc cgtcattctg gcgcccggac tggaaattcc cggtgccgcc ctgattaacg
     4381 actcgtcatg cgtagccctc ggccattaca ggacgcggaa ctcgggactg tgcagtcaga
     4441 agcttgacct gctgtcgcgt gacatcttct ccaaggaaga gcgcaggcag ctgcttgcca
     4501 cctttactca cgacctgagc gatcgggtgg agatctatgg gcacttcggc ggctcggaaa
     4561 cggacgtgcg catcaatcag tcaccgtcgt ttcccatcac ctcgcaggtt cccttctcat
     4621 cggcgaaccc gggcttccag ttcgaggtgc tgaacggttt tcaatccctc ctcacaacgg
     4681 gcaccgcgcc attcggggac gacgtgaaca accggctggc gtcgctgctg gcgggccaac
     4741 cttatccgct gcccgtgctg ccgttcgttc cgcccgagct cggcggtccg aacccgctgg
     4801 atccattcgc gattttgtcg caggtcggga ctctcacttt caatggggtg atgcgcccgt
     4861 cgctgctgca cttagcgcag atcgccgggg tgccggcaga cgttgacggt gatggcgtgg
     4921 tggaggaagg cgagatcttc cgtaaccgca accagagcac catcgacagg gagacccggg
     4981 tgttcatgat cggcgcacgg ggagacctga acgacgcctg gagcttcgat acctcgttca
     5041 actacagcag cgagaagagt attacggtct tttacgatac ggttacggac cgcctttccg
     5101 atgcgttgaa cgggtggttc ggccttggct gcgacaagga cgcttacggg ccgggcgaag
     5161 gaccttgcac ctggttcaac cccttcggca gctccatgct tctgcccgac gtcgtatttc
     5221 ccgacgggaa cgggaacctg cataccctcg gcaacgatgc ggccgccgtg cacgcgctgg
     5281 aaggggaggg agtagcgacc gcgcgcagct ccctgatcgt ggtggacgca atcatgtcga
     5341 ccaacgagtt tttcggctgg gagctaggag gcggtggcgt cggctttgct attggcgccc
     5401 agtaccgcaa agaggagtac aaagcgggcg gcaacgagct cgccacggat ccgagctttc
     5461 cttttgcgtt cacgggcccg acgatcccct tcgacgcgga tcaggacatc tgggccatat
     5521 tcacggaatt tgccctgccg ctgaccgagg acttagaggt tcagctggca ctgcgttacg
     5581 aggactatgg cgacgatacg ggcgacacga tcgaccccaa ggtcgccgtt cgctggcagg
     5641 cggctgacag cctggtcctg cgcgcgtccg ccggtacctc tttccgcggc ccgtccctgg
     5701 tgcagaaatt cggcaccggc acaggattgc agttcatcct gcccccgtcg cccgaagtca
     5761 ttggggccaa cgtgccggga cagacgttcg gcagcggtgt gttcgcccga ctgcccacgt
     5821 atggcaaccc ggggctgcag cccgaggagt cggtgaactt caatattggc gtgatctggt
     5881 cgcccaatga aaatctgagc atctcgctgg actacttcaa ctacgactac gaggacgtga
     5941 tcgtgcctga tgacttccgg ggtctggcca acgactgcca gatcaactgg ggtcttgcag
     6001 gtcgcccggt gccccagaat gtcgatggca gcttcaacgc tgattatctt gctgtcgagg
     6061 cctgcaactt cctggatctg gacggggatc cgggcacacc agacattttc ctggatactc
     6121 agggcaaccc gctcaccgtc
//
LOCUS       DBNHLM010000166         9028 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1580624, whole genome shotgun sequence.
ACCESSION   DBNHLM010000166 DBNHLM010000000
VERSION     DBNHLM010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9028
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1580624"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1568
                     /locus_tag="AC16QF_07560"
     CDS             <1..1568
                     /locus_tag="AC16QF_07560"
                     /inference="COORDINATES: protein motif:HMM:NF016348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HIR6448451.1"
                     /translation="TVVPRYIAERGLNLETEFRHLNRWQGTTLSGAILPKDDLYDGTY
                     RKKDFEDLVEAGELAGEFEPANRWLYGMQHRGRLGRFRSYVDYTAVSDRDYFRDLGSD
                     LGISSQIDLERRGELQYNSGGLFARVWAQRFQRLDEVTVDPYQRLPEVDVTYTGDLAG
                     RLEYSLGAQWVSFTRDNEDLGGFGAVVGDRLHLEPRVRVPFSWPWGFLNFSGGYRYTK
                     YDLEDVPVGVEEKPDRGIGLGSADAGLYFDRDLELFGTELVQTLEPRVFYLYQEYEDQ
                     STLPNFDVSKLTFGYSQLYRDNRFSGLDRIGDSNQLSIGLTTRFIDGANGREYLRASI
                     GQIVYFRDRLVTLGGPTDDDQDGSSALAGELSFALARAWRLTGTLVWDVKDNQIDEAA
                     AALTYRRDSRHMFNLGYRKRLESNIHQTDAALYWPLGRRFGLIGRWNYDIESGRTIEG
                     LAGIEYNNCCWQLRLVARRYIDSPSARQIATVDADKGIFFQFVFKGLAGFGDRLEAVL
                     RRGIRGYSTEVYD"
     gene            1601..2878
                     /locus_tag="AC16QF_07565"
     CDS             1601..2878
                     /locus_tag="AC16QF_07565"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6448452.1"
                     /translation="MSLTLGLLTASALASAPALAEYRELDAIVAVVDEDVVLASELLS
                     RLKSIREQFAEQQGQLPSDDILVSQLMERLIIESLQMQEAERRGVQIDDETLTRAVMS
                     FAERNGMSAEQFRQALAADGVSYVEFREQIKQEMTLQRLQRNLVNRRITITDSDIDDL
                     LNSPYYQQLLSDEFRVGHILLAIDDKATEEAIQEAARQAVEIVEELRAGADFAQIAVA
                     RSAGSRALEGGDLGWRRAGELPSLFADQVLALEPGETAEPVRSASGIHIVQLLEQRGA
                     GMQKEEQTLLRHILVMPSEIRSEEEAEALIRQVHDELEAGGDFVALAAEHSEDPGSAL
                     NGGDLGWTSGNEFVPAFREAMYATTTGELSEPFRSQYGWHVLEVQERREQDLSEETRR
                     NMAMQILHQRRFDEELQEWLKELRDEAFVEMRL"
     gene            2884..3864
                     /gene="pdxA"
                     /locus_tag="AC16QF_07570"
     CDS             2884..3864
                     /gene="pdxA"
                     /locus_tag="AC16QF_07570"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR6448453.1"
                     /translation="MPISLAITPGEPAGVGPELLLRLAESPRTRPWVAVADADLLAER
                     ARALGTTVRLDDAWDRPAMEAGRLTVRHVPLEAPCTPGQLDPRNAAYVLETLRLATDG
                     CLSGEFAGLVTGPVQKSVMNDAGIPFTGHTEFLCERAGVADVLMLLVAGTLRVGLATT
                     HLPLREVPDALSRALLEGRLGLLLDGLRRDFGIARPRVLVAGLNPHAGEGGHLGTEEI
                     EIIDPVCERYRAAGEQVKGPLPADTLFTPAMLDTADAVLAMYHDQGLPVLKHAGFGRA
                     VNVTLGLPFVRTSVDHGTALDLAGSGVADPGSFAAAASLAGDLLDFRASA"
     gene            3911..4627
                     /gene="rsmA"
                     /locus_tag="AC16QF_07575"
     CDS             3911..4627
                     /gene="rsmA"
                     /locus_tag="AC16QF_07575"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003206170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR6448454.1"
                     /translation="MADLIRLGPHDQVLEIGPGTGALTAALLDASGAAPARYLAVEID
                     RDLAPELGRRFPAVEVVNADVLRVPLDELLGSQSWRVVGNLPYNISTPLLVRLFACTD
                     RIRDMHFMLQREVALRLAAVPGTKAWGRLTVLAQYHCQVELLFEVAPESFSPPPKVWS
                     AVARLVPHAAKLPLENPDLLDLVLRHAFSGRRKRLGNALKSLSMPWHELDVDDAARPD
                     QLSVKDFVTLANAVQLEQTP"
     gene            4624..5004
                     /gene="apaG"
                     /locus_tag="AC16QF_07580"
     CDS             4624..5004
                     /gene="apaG"
                     /locus_tag="AC16QF_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HIR6448455.1"
                     /translation="MSGPASGIEVAVDARYVVGESDPIRNRYVFAYTITISNLGPEPG
                     QLLDRHWFIADGDNEVREVQGPGVVGEQPRLEPGTAFRYTSAAVLETPVGTMHGLYGF
                     RRDDGTRFEVPIPMFSLAAPNAVH"
     gene            5014..5826
                     /locus_tag="AC16QF_07585"
     CDS             5014..5826
                     /locus_tag="AC16QF_07585"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HIR6448456.1"
                     /translation="MATYAIGDVQGCYAEFCRLLKKMEFDKKRDRLWMVGDLINRGPD
                     SLRVLNLVRSLGKRAVIVLGNHELHFLAIHYGGYSANRTDTFDDLLSSPARAEIAEWL
                     CQQPLLVRSNKLGYVMTHAGIPHIWDIDVAEALAGEVEKAIRGKRRKRYFESMYGNVP
                     DVWDPKLAGMDRLRCITNYFTRMRLVDKAGRLDFSFKGGLADAPKPWLPWYELRNRKP
                     LPVKLVFGHWAALQGHTGLKDVIALDTGCVWGRKLSALCLDTGEKYSVRAGG"
     gene            6118..8040
                     /locus_tag="AC16QF_07590"
     CDS             6118..8040
                     /locus_tag="AC16QF_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HIR6448457.1"
                     /translation="MGVFEHYQERFEAAREEELSLQEYLDLCKEQPATYATAAERLLL
                     AIGEPEFVDTAKDSRLSRIFSNKIIKRYPAFDDFYGMEEAIEQIVSFFRHAAQGLEEK
                     KQILYLLGPVGGGKSSLAEKLKALMQREPFYALKGSPVNESPLGLFDPVEDGAILESD
                     YGIPRRYVRGLMSPWAAKRLKEFGGDITQFRVVKKYPSILDQVALSKTEPGDENNQDI
                     SALVGKVDIRKLEDFAQNDPDAYSFSGGLCKANQGMLEFVEMFKAPIKVLHPLLTATQ
                     EANYNGTEAIGSIPFDGIVLAHSNESEWQTFRNNRNNEAFIDRVYIVKVPYCLRVREE
                     VNIYRKLIDTSSLNAAPCAPDTLRMLAQFSVLSRLKEPENSNVFSKMRIYDGENLKDT
                     DPKAKTLQEYRDTAGVDEGMDGLSTRFAFKILSKVFNFDAIEVAANPVHLMYVLEQQI
                     EQEQYPQEVRDRYLRFIKEYLAPKYVDFIGKEIQTAYLESYSEYGQNVFDRYVTYADF
                     WIQDQEYRDPETGDILDRASLNEELEKIEKPAGISNPKDFRNEIVNFVLRARASNNGT
                     NPSWLSYEKLRTVIEKKMFSNTEDLLPVISFNAKSSSDEQRKHDDFVARMVERGYTDK
                     QVRLLAEWYLRVRKSQ"
     gene            8116..>9028
                     /locus_tag="AC16QF_07595"
     CDS             8116..>9028
                     /locus_tag="AC16QF_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HIR6448458.1"
                     /translation="MSYIIDRRENAKNKSAVNRQRFLERYKQRIKKAVSDAVNERSIT
                     DMERGEEVTIPAQDISEPVFQHGSGGRRSIVHPGNKEFIAGDRIARPEGGAGGGSGDG
                     EASNSGEGSDDFVFQLTQEEFLEYLFDDLALPNLTKRHLKGNSSFKYEHAGFTSDGVP
                     AKLSVLRSLRQAKARRIALAGEPKRLLREAREAFDQAELDGDELQRRRMRDEIERLEK
                     RLGRIPFLDTNDLRYNLHLRKPVPTSQAVMFCLMDVSGSMDQHIKDMAKRFYLLLYMF
                     LKRHYERTEIVFIRHHTVAKEVDEQEFF"
BASE COUNT         1652 a         2882 c         2945 g         1549 t
ORIGIN      
        1 ccaccgtggt gccgcgctac atcgccgagc gggggttgaa cctcgaaacc gagttccggc
       61 acttgaaccg ctggcagggc acgacgcttt ccggcgccat cctgccgaag gacgacctct
      121 acgacggcac ctaccgcaag aaggacttcg aggatctggt ggaggccggt gagctcgccg
      181 gcgagttcga gccggccaat cgctggctat acgggatgca gcatcggggc cggctcggcc
      241 gcttccgctc ctacgtggac tacaccgcag tcagcgatcg cgattacttt cgcgatctcg
      301 gctcggacct gggcatatcc agccagattg acctcgagcg gcgcggcgag ctgcagtaca
      361 actcaggcgg cctctttgcc cgggtctggg cgcagcggtt tcagcgcctg gacgaggtca
      421 ccgtggatcc gtaccagcgt ctgcccgagg tggacgtgac ctacaccggc gatctggccg
      481 gtcgcctgga gtactcgctg ggcgcgcaat gggtgtcttt tacccgggac aacgaggatc
      541 tcggcgggtt cggggcggtg gttggggacc ggctgcatct ggagccgcgg gtacgcgtgc
      601 ccttcagctg gccctggggc ttccttaact tcagcggcgg ctaccgttac acgaaatacg
      661 atctcgagga cgtgccggtg ggcgtggaag agaaacccga ccgtggcatc ggcctcggca
      721 gcgccgacgc ggggctctac ttcgaccggg acctcgagct cttcggcacg gagctggtgc
      781 agaccctgga gcccagggtg ttctatctgt accaggaata cgaggatcag agcacgctgc
      841 ctaacttcga cgtgagcaag ctcaccttcg gttacagcca gctgtatcga gacaaccgct
      901 tttcgggcct cgaccggatc ggcgattcca accagctctc catcggcctc accacccgct
      961 tcatcgacgg cgccaacggg cgcgagtacc tcagagccag catcggccag atcgtctact
     1021 tccgggatcg gctcgtgacc ctcggtggcc ccaccgacga cgatcaggac ggctcctccg
     1081 ccctggccgg ggagctgtcg tttgcccttg cccgcgcttg gcgcctcacc gggaccctgg
     1141 tctgggacgt caaggacaac cagatcgacg aggccgccgc agcgctcacc taccggcgcg
     1201 acagccggca catgttcaac ctgggctacc gcaaacgcct ggagagcaac attcaccaga
     1261 ccgatgccgc cctctactgg ccactcggac gtcgcttcgg cctgatcggc cggtggaact
     1321 acgacatcga gagcggtcga acgattgagg gcctggcggg aatcgagtac aacaactgct
     1381 gctggcagct gaggctggtg gcacgacgtt acatcgacag ccccagcgcg cggcagatcg
     1441 ccacggtgga cgcggacaaa ggcatcttct tccagttcgt cttcaagggc ctggcggggt
     1501 tcggcgatcg gctcgaggcg gtgctgcgcc gcggcatccg gggatacagc acggaggttt
     1561 acgactgatg gttggtttgg gaagcggatc gcgacgggtg atgagcctga cgctcgggtt
     1621 gctgacggcc tctgcgcttg cgagcgcgcc ggcgttggcc gagtaccggg aactcgacgc
     1681 catcgtggcg gtggtggacg aggacgtggt gctcgcctcg gagctgcttt cgcgcctgaa
     1741 aagcatccgc gagcagtttg ccgagcagca gggtcagctg ccgtcggacg acattctggt
     1801 cagccagctc atggagcggc tcatcatcga gagcctgcag atgcaggagg ccgagcgccg
     1861 cggcgtgcag atcgacgacg agaccctgac cagggcggtg atgtcgtttg cagagcgcaa
     1921 cgggatgagc gcggagcagt tccgtcaggc attggccgcc gacggggtca gctacgtgga
     1981 gtttcgcgag cagatcaagc aggagatgac cctgcagcgc ctgcagcgca acctggtgaa
     2041 ccgtcgcatc accatcaccg acagcgacat cgacgatctg ctgaactcgc cctactacca
     2101 gcagctgctt tccgacgagt tcagggtcgg gcacattctg ctggccatcg acgacaaagc
     2161 gaccgaggaa gccatccagg aggcggccag gcaagcggta gaaatcgtcg aggagcttcg
     2221 tgcgggcgcg gacttcgctc agatcgccgt ggcccggtcg gcggggagtc gggcgctgga
     2281 aggcggcgat ctcggctggc ggagagccgg agagctgccg agcctctttg ccgatcaggt
     2341 cctggcactc gagcccggcg agaccgccga gcccgtccgc agcgccagcg gcatacacat
     2401 cgtccagctg ctcgagcagc ggggcgcggg catgcagaaa gaagagcaga cgcttctgcg
     2461 tcacatcctg gtcatgccct cggagatccg ctcggaagag gaagccgaag cgctcatccg
     2521 ccaggttcac gacgagctcg aggccggcgg cgatttcgtc gccctggcgg ccgagcactc
     2581 ggaggatccg ggcagcgcgc tcaacggcgg tgacctgggc tggacgtcgg gcaacgagtt
     2641 cgtgcccgcg ttccgcgagg cgatgtatgc gaccacgacg ggcgagctgt ccgaaccgtt
     2701 tcgctcccag tacggctggc acgttctgga ggttcaggag cgacgcgagc aggatttgag
     2761 cgaagagacc cgccgcaaca tggcgatgca gatcctccat cagcgccgct tcgacgagga
     2821 gctccaggag tggctgaagg aactgcggga cgaagcgttc gtggagatga ggctctagcg
     2881 ggtgtgccga taagcctcgc catcacgccc ggcgagccgg cgggcgtcgg cccggagctt
     2941 ctgctccgac tggcggaaag tccccgcacg cgcccctggg tagcggtggc cgacgcggat
     3001 cttctggccg agcgggcccg cgccctgggc accacagtcc ggttagacga cgcctgggac
     3061 cgcccggcca tggaagccgg gcggctgacc gtgcggcacg tgcctctcga ggctccttgc
     3121 acccccgggc aactcgatcc ccgcaacgcc gcctacgtcc tggaaacctt gcggctggcg
     3181 acggacggtt gcctgtcggg ggagtttgcc ggccttgtca cgggacccgt gcagaagtcg
     3241 gtaatgaacg acgcgggcat cccgttcacc gggcacacgg agttcctgtg cgagcgggcg
     3301 ggcgttgccg acgtgctcat gctgctggtg gcgggcacgc ttcgggtcgg gctcgccacg
     3361 acccatttgc ccctgcgcga ggtgcccgac gccctttccc gagctctgct cgagggccgg
     3421 ctcggcctgc tgcttgacgg gctgcgaagg gatttcggta ttgcccgacc ccgggtactg
     3481 gtcgccggtt tgaaccccca cgcgggggag ggcgggcacc tgggtaccga ggagatcgag
     3541 atcatcgatc ccgtgtgcga gcgctaccgc gccgccgggg agcaggtgaa ggggccgctg
     3601 cctgccgaca ccctcttcac cccggccatg ctcgacaccg cagatgcggt gctcgccatg
     3661 tatcacgacc agggtcttcc ggtgctcaag cacgccgggt tcgggcgggc ggtcaacgtc
     3721 acgctcggcc ttcccttcgt gcggacgtcg gtcgaccacg gtaccgcgct tgacctggcg
     3781 ggctccgggg tggctgaccc gggcagcttt gccgccgccg cctcgctggc gggcgacctg
     3841 ctggatttcc gtgcgagcgc gtaagcggtt cggccagcac tttctcaccg acgaagcggt
     3901 gctgcagcgc atggcggatc tgatccgtct cgggccgcac gaccaggtgc tggagatcgg
     3961 tccgggcacc ggggcgctca ccgccgctct gctcgacgcc tccggagcgg cgccagcgcg
     4021 ttacctggcg gtggagatcg accgcgatct ggcccctgag ctcgggcgcc gcttccccgc
     4081 cgtggaggtc gtcaacgccg acgtgctgcg cgttccgctc gacgagctgc tgggctcgca
     4141 gagctggcgc gtggtgggca atctgcccta caacatctcc acgccgcttc tggttcgtct
     4201 gttcgcctgc accgaccgaa tccgcgacat gcacttcatg ctccagcgcg aggtggcgct
     4261 gcgcctcgcg gccgttccgg gcaccaaggc atggggccgg ctgacggtgc tcgcccagta
     4321 ccactgccag gtggagctgc tcttcgaggt tgcgccggag agtttcagcc caccgccgaa
     4381 agtctggtcc gccgtcgcgc ggctggtgcc ccatgcggcc aagctcccgc tcgagaaccc
     4441 ggacctgctg gatctggttc tgcgccacgc gttctccggg cggcgcaaac ggctcggcaa
     4501 tgcactaaaa tccttgagca tgccctggca tgaactcgat gtggatgacg ccgcccggcc
     4561 cgaccagctg tcggtgaagg atttcgtcac gctcgccaac gccgtgcagt tggagcagac
     4621 accgtgagcg gacccgccag cggcatcgag gtggcggtgg atgcgcgcta cgtcgtcggc
     4681 gagtcggatc cgatccggaa ccgctacgtg ttcgcctaca ccattacgat cagcaacctc
     4741 gggccggagc ccggtcagct cctcgaccgc cactggttca tcgccgacgg cgacaacgag
     4801 gtgcgcgagg tgcaggggcc gggagtggtc ggcgagcaac cccggctcga gcccggcacc
     4861 gccttccgtt ataccagcgc cgcggtttta gaaacgcccg tgggaaccat gcacgggctc
     4921 tacggcttcc gccgtgacga cggcacgcgc ttcgaggtgc cgattcccat gttctcgcta
     4981 gccgcgccca acgcggtgca ttgagaccgg gcagtggcca cctacgcgat cggtgacgtt
     5041 cagggttgct acgcggagtt ctgccggctg ctcaagaaaa tggagttcga caagaagcgc
     5101 gatcggctct ggatggtggg tgatctgatc aaccgtgggc ccgattcgtt gcgggttctg
     5161 aacctggtgc gttccctggg caagcgcgcg gtcatcgtgc tcggcaacca cgagctccat
     5221 tttctcgcca tccattacgg cggctacagc gccaaccgga ccgacacgtt cgatgatctg
     5281 ctgagctccc cggcccgcgc cgagatcgcc gagtggctgt gccagcagcc gcttctggtc
     5341 cggagtaaca agctgggcta cgtcatgacc catgccggta ttcctcatat ctgggacatc
     5401 gatgtcgcgg aagctctggc cggcgaggtg gaaaaagcca ttcgcggcaa acggcgcaaa
     5461 cgctacttcg aaagcatgta cggcaacgtg ccggacgtgt gggatccgaa gctcgcaggc
     5521 atggaccggc tgcgctgcat caccaactac ttcacccgca tgcgtctggt ggacaaggcg
     5581 ggacggttgg atttttcctt caaagggggg ctggccgacg cgcccaagcc ctggctgccg
     5641 tggtacgagc tgcgcaaccg caaaccgctg ccggtcaagc tggttttcgg tcactgggcg
     5701 gcgctgcagg ggcacacggg gctgaaggac gtgatcgccc tggatacggg atgcgtgtgg
     5761 ggccgcaagc tcagcgccct gtgcctggac acgggcgaga agtattcggt tcgggcgggc
     5821 ggctgaggtg acaggggccc cgggtgggcc gtaacaaagc cgttttcttg cccggtccgg
     5881 caactccggc gcagcacgga tcggttcaga ggcgacttga gtcccattcc ttgggtttcg
     5941 ccgttccaaa tgtcaacgaa tcacgcgttc cgtaccgccg ataaggcgga attagccgct
     6001 catccattag tgattccggt cgcaggtgcg tgtgtgacac ggttcacgga tcgacgctga
     6061 atgcaacggt agcttcagtg aggtacacgc ggatgcagca aaggagcgct agcaaaaatg
     6121 ggtgtattcg agcactatca ggaacgattc gaagcggctc gcgaagaaga gctttccctt
     6181 caggaatacc tggatctctg taaagaacaa cccgccacct acgccacggc cgccgagcgg
     6241 ctgctgctgg cgatcggcga gccggagttt gtcgacaccg ccaaggattc gcggctttcg
     6301 cgcatctttt ccaacaagat catcaagcgc tacccggcgt tcgacgactt ctacggcatg
     6361 gaagaagcca tcgagcagat cgtgtctttc ttccgccatg cggcccaagg cctcgaggag
     6421 aaaaagcaga ttctctacct cctcgggccc gtcggcggcg gcaagagctc gctcgccgaa
     6481 aagctcaaag cgctcatgca gcgcgaaccg ttctacgcgc tgaagggctc gccggtgaac
     6541 gagtcgcctc tggggctgtt cgatcccgtt gaggacggcg ccatcctcga gtccgactac
     6601 ggcatcccgc gccgctacgt tcggggactc atgtcgccat gggcggccaa gcggctcaag
     6661 gaattcggcg gcgacatcac ccagttccgg gtggtgaaga aatacccgtc gatcctggac
     6721 caggtggcct tgtcgaagac cgagcctggt gatgagaaca accaggacat ctctgccctg
     6781 gtgggcaagg tggacatccg caagctcgag gacttcgcgc agaacgaccc ggacgcgtac
     6841 agcttctccg gcgggctgtg caaggccaat cagggcatgc tcgagttcgt cgagatgttc
     6901 aaggcgccca tcaaggtgct gcacccgctg ctcaccgcga cccaggaagc caactacaac
     6961 ggcaccgaag ccatcggctc cattccgttc gacgggatcg tgctggccca ctccaacgag
     7021 tcggagtggc agacgttccg taacaaccgc aacaacgaag ccttcatcga ccgcgtgtac
     7081 atcgtgaagg tgccctactg cctgcgggtc cgggaggaag tgaacatcta ccggaagctg
     7141 atcgatacca gctctctgaa cgcggcgccc tgcgcccccg acacgctgcg catgctggcg
     7201 cagttctcgg tgctttcccg gctcaaggag ccggagaact ccaacgtgtt ctccaagatg
     7261 cgaatctacg acggggaaaa cctcaaggat acggacccca aggccaagac gcttcaggag
     7321 taccgcgaca cggccggtgt cgatgagggc atggacggtc tgtccacccg cttcgccttc
     7381 aagatcctct cgaaggtgtt caatttcgac gccatcgagg tggccgccaa ccccgttcac
     7441 ctgatgtacg tgctcgagca gcagatcgag caggagcagt atcctcagga ggtccgggac
     7501 cggtatctgc gcttcatcaa ggagtacctg gctcccaagt acgtggactt catcggcaag
     7561 gaaatccaga cggcgtacct cgagtcctac tccgagtacg gtcagaacgt cttcgaccgc
     7621 tacgtcacat acgccgattt ctggattcag gatcaggagt atcgcgaccc ggaaaccggc
     7681 gacattctcg atcgcgcttc cctgaacgag gagctggaga aaatcgagaa gcccgccggc
     7741 atcagcaatc cgaaagactt ccgcaacgag atcgtcaatt tcgtgctgag ggcgcgtgca
     7801 agcaacaacg gcaccaaccc gtcgtggttg agctacgaga agctgcgcac ggtcatagag
     7861 aagaagatgt tctccaacac cgaggatctt ctgccggtga tctctttcaa cgcgaagtcc
     7921 tccagcgacg agcagcgcaa gcacgacgac ttcgtcgccc ggatggtgga gcgcggttat
     7981 accgacaagc aggtgcgact gctcgcggag tggtacctga gagttcgcaa gtcacagtag
     8041 caacggaacc ctctcaccgt cccggaggct gatgcccccg ggacggtgag aggcaccgcg
     8101 cagcgggggc atggcatgag ctacatcatc gaccgccggg aaaacgccaa gaacaagagc
     8161 gccgtcaatc ggcagcgttt tctggagcgt tacaagcagc gcatcaagaa ggcggtatcg
     8221 gatgcggtga acgagcgcag catcaccgac atggagcggg gcgaggaagt caccatcccc
     8281 gcccaggaca tctccgaacc ggtctttcag cacggcagcg gcgggcgccg cagcatcgtg
     8341 catcccggca acaaggagtt cattgccgga gaccgcattg cgcgtccgga aggcggcgcc
     8401 ggcggcggtt ccggggatgg cgaggcctca aactctggtg agggttccga cgacttcgtc
     8461 tttcagctca cccaggagga gttcctcgag tacctcttcg acgacctggc cctgcccaac
     8521 ctcaccaagc ggcatctgaa aggcaactcg agcttcaagt acgaacatgc cgggttcacc
     8581 agcgacgggg tgcccgccaa gctctcggtg ctgcgttccc tgcggcaggc gaaggcgcgg
     8641 cgcatcgcgc tggccggcga gcccaagcgg ctgcttcgcg aggcgcgcga ggcgttcgat
     8701 caggcggagc tcgacggcga tgagctccag cgtcgccgca tgcgcgacga gatcgagcgc
     8761 ttggaaaaac ggctcggccg catacccttt ctggatacca acgatctgcg ctacaacctg
     8821 cacttgcgca agccggtgcc cacctcccag gccgtgatgt tctgcctcat ggacgtgtcc
     8881 ggctccatgg accagcacat caaggacatg gcgaagcgct tctacctgtt gctctacatg
     8941 ttcttgaagc gccattacga acgcacggag atcgtcttca tccggcatca caccgttgcc
     9001 aaggaggtgg acgagcagga gttcttct
//
LOCUS       DBNHLM010000167        11089 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1221865, whole genome shotgun sequence.
ACCESSION   DBNHLM010000167 DBNHLM010000000
VERSION     DBNHLM010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11089)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11089)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11089
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1221865"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="AC16QF_07600"
     CDS             <1..675
                     /locus_tag="AC16QF_07600"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR6448459.1"
                     /translation="LGAILAALRSAERTGEGQVVETSLFETAIWTQASDFGTTAIDRA
                     PVRRRARHNMLSPTANRYPCGDGKWLVVNMLDAMAFDRFCRAIGREDWLEDERFREAR
                     GRYDHMPELVTGIDATLATRSRDEWGRIFDEAGLIWGPVLTLDEAAGDGQAHAIGMFP
                     ELEHPEYGTYRTVNAPFRFRSGEVRPRGTSPGPGEHTREVLGEMDFDEAEIDALMAAG
                     VLPTHD"
     gene            complement(672..986)
                     /locus_tag="AC16QF_07605"
     CDS             complement(672..986)
                     /locus_tag="AC16QF_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448460.1"
                     /translation="MNHPVTQRSLRQQNQAFSGTNGVSQNNRSMRFLPAFRDEETGQV
                     ELARFDDGRVAPAHLIVGLPRRWAVAFASDGAILALKPSITAGFVRDDVFFTREEAAS
                     AD"
     gene            complement(1568..2218)
                     /locus_tag="AC16QF_07610"
     CDS             complement(1568..2218)
                     /locus_tag="AC16QF_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448461.1"
                     /translation="MTRPAAPRLPSGLLLALLLAPQVASGHGGVVLEEDQCVLKMDFL
                     LAHFTGYQPESRGSEEFCEDIPEVGESLFVLEYMHDFMKEMPVDFRIISDDTELGTFA
                     NWDDVHALGDLTPRTVFYQPPRKQADGVLTASHRFDQAGGYIGIVTASHPTQEKIYNA
                     VFFFRVGGPDLGYLPLFVGLIILVQLIYWGATGTLQRFLRRYLPNRRNYGEAAAAG"
     gene            2316..3299
                     /locus_tag="AC16QF_07615"
     CDS             2316..3299
                     /locus_tag="AC16QF_07615"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR6448462.1"
                     /translation="MATIAILGLGNFGTALARVWLLGGHDVRGWTVEEDVFDAIRSKG
                     SNSKYLDGIDLTGLQVSMELGESVPGAELVVLALPSHVVLHVADDVIPLLTPDQVVLD
                     LAKGIAGEELVSDAIQAKLAAAGKTNPLAVITGPTIAPELASGVLTTALVASEDESLA
                     SRLAEQLSTSTFSLKAASDPRGAELWGGFKNVIALGCGIADGLDEGSGDNLKAAIFTA
                     GFAEGNRLLTALGAAPETAFTPAGIGDLFVTASSPHGRNRSLGQKLGAGQTLEEAMAG
                     SVMVAEGVRATRMFAELAAARSVTTPFIAALTDLLDGNCTAPDCVRRLMAI"
     gene            3395..3694
                     /locus_tag="AC16QF_07620"
     CDS             3395..3694
                     /locus_tag="AC16QF_07620"
                     /inference="COORDINATES: protein motif:HMM:NF018717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR6448463.1"
                     /translation="MAAFFVAQYTVNNPALYAEYQQGAGPTLATYGAELVSFDVGAET
                     VEGNPPGPQTVIIKFESTDAAKAWYNSAEYQAVVGKRLEATDGFAVIAQSMNAGG"
     gene            complement(3728..5059)
                     /locus_tag="AC16QF_07625"
     CDS             complement(3728..5059)
                     /locus_tag="AC16QF_07625"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spinster family MFS transporter"
                     /protein_id="HIR6448464.1"
                     /translation="MTDQVEEQPAGAPAAQGFSGKTPWRSWYALGVLFVVYVFNFIDR
                     SILGILNQAIKEDLALSDTQMGFLGGIAFAVFYTFLGIPIARLADRTVRRNLLAVCLA
                     IWSVMTALCGLASSFAHLLLARIGVAVGEAGGSPPSHSMISDMFPESSRATALSIYAL
                     GIPVGTMIGNLAGGWINEAFDWRTAFIVVGLPGVALAFLVVLTVREPVRGAADNMTAS
                     SEAAPPVMTVFRYLWSLKSFRYLSLAGALHAFVGYGVGYWFPAFFIRSHGLGTGEIGT
                     WLFYLGFAGMAGTFLGGYMGDRMARRDVRWYVWLPGIATLLSVPFSMVVYLYDDYRVA
                     FLVAIVPTLLGAYYLGPTFALTQGLVGLRMRALASSILLFILNLIGLGLGPQVTGILS
                     DGFNAFGDLGTDSLRYALICVLLLNVVATVYYWMAGRDLERDLARRGELDG"
     gene            complement(5052..5687)
                     /locus_tag="AC16QF_07630"
     CDS             complement(5052..5687)
                     /locus_tag="AC16QF_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HIR6448465.1"
                     /translation="MADYPSQRGIVLWAAPFLAVLLWSAVNPKDYEVWVLEVSPAVAG
                     ALIIFLTRRRFPLTRLTYGLILVHALILIVGGHYTYAEVPLFDLLREPLGFTRNNYDK
                     LGHLAQGFVPAIVAREVLLRLSPLERGGWLNLFVVSLCLGISAFYELIEWWVALLSEQ
                     AAESFLGTQGYVWDTQSDMGWALLGAVLALVLLARYHDRQLLEQGLAGNHD"
     gene            5781..7412
                     /locus_tag="AC16QF_07635"
     CDS             5781..7412
                     /locus_tag="AC16QF_07635"
                     /inference="COORDINATES: protein motif:HMM:NF004807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HIR6448466.1"
                     /translation="MKVAQAIAKALKAEGVEVLFAYPVNPIVEAAAAEDIRTVIVRQE
                     RVGLHMADAYSRLSSGDRIGVFAMQHGPGAENAFGGVAQAYSESVPILVLPAGYHRRI
                     ANYYPNFSSTLNMRNITKWAEPLTMGKAVPEVMRRAFFQLRNGRPGPVLVEIPVDVFR
                     EDVPDDWHHTPSFKTRSAPDPDAVATAAEVLAKAERPVIYAGQGVHYAKAWAELKALA
                     ETWNIPVTTSIEGKSAFPENHPLSLGSGGRANPRPVKAFLNEADVILGIGCSFALTGF
                     GVPMPAGRTIIHATLDPMDLNKDVPAQHGLIGDAALTLRALSQAMDGKDRSPAEARAG
                     VSGRIAAIREEWLGEWMPKLTNDEAPINPYRVLWELNQMVDKDNTVITHDAGSPRDQT
                     TPFWECTTPLSYIGWGKTTQLGYGLGLAMGAKLACPEKLCINVWGDAAIGFTGMDFET
                     AVRERIPILSILFNNFSMAIEIPIMQISQAKYGATDISGNYADMAKAFGGYGERITDP
                     GDIRAALERGIEATRAGSPALLEFITDKEITVSNE"
     gene            complement(7430..8662)
                     /locus_tag="AC16QF_07640"
     CDS             complement(7430..8662)
                     /locus_tag="AC16QF_07640"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6448467.1"
                     /translation="MDRGLRWYLLSTGLFLVPGGIQMVLFPWLVAVYLQESPARVGIA
                     QMSLQLPMLLLILWGGLVGDRFDQRRLLVGLHLGMIGPPLAMAVLVQADLIVYEVLLG
                     WAVIGGSFGAFAQPARDALLNRVAGGDVQGVVTLAIGVQFGVQIIGFALGSTADSLGP
                     LWLFLVQALLMGAAAVATSKIPLLPMGSARERRPALREIGEGIVMAWRSEAIRPAVLL
                     TFAQGVFFAGAYLVILPLMVRDLYGGGAGGIAGAFAANMLGTCTVVFFLRRRGGIDRP
                     GRALGLWGVFGVCVLSLLHFELPLWGFYAVIYLWGTCGGVSMTMSRSIVQEASPPSHR
                     ARLMSVYSLGLMGGMPFGSAFLGWCVGVLGARNAVLVPVIGMAVVQLLIFTTSRLWLV
                     ERQRGSDLDEEPLMHAAD"
     gene            complement(8666..10039)
                     /locus_tag="AC16QF_07645"
     CDS             complement(8666..10039)
                     /locus_tag="AC16QF_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HIR6448468.1"
                     /translation="MTSTFPGVIAFAFMVCMLLAGTVLRAKVPLLRRALVPASLVGGI
                     LGFALVSAGLSFGYESSDFTVFTFHFFTLSFMSLCLTGGSRERGKNTSIIPGGMWMSV
                     VWVVSLVTQGLVGLGALVGYNVLTGGELSSFLGLLATHGFTQGPGQALAYASIWESEL
                     GVVHAINFGIIYASAGFIVAFAVGVPVARHAIKAGRNVNRVARIDDEFQAGILNADSD
                     VSAGRQITHSANIDTLAFHIAVLGVAYLVTDQYLSFMRPIADGWAIGDVNFGVLFSHN
                     LFFVHGLMVCVIMRSLLDRFGLGHYIDDETQRRITGTSVDFMVVATIMSIHFGLLSEF
                     IVPILVVCLAVSAVTVVLCFGLGRHLPNLSAERSLTSFGCCSGSTGSGLLLLRIVDPD
                     LSTGIAKELAFFNIAILVLSFHVLGFMAPILPSYDLTFICAVYAATLVVGLVAVRMVR
                     KRMMAAA"
     gene            complement(10082..>11089)
                     /locus_tag="AC16QF_07650"
     CDS             complement(10082..>11089)
                     /locus_tag="AC16QF_07650"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6448469.1"
                     /translation="REHDLVEGCLDVFRHANSVGFTTICDQGTGAMQGAKELALYEAL
                     RESNRMTARFRYSLFNTLAADWDASDVSFGTGDSWVRASAWKIVSDGSNQGLSGLQRE
                     PFMNADSHGIAYIEPEALNEAVKRRLGQGWPVAVHANGDLAIDRVLDAFEAARAAGLD
                     PARARCRIEHCSILHDEQIERIADLGLSPSFLIGHVYWWGKAFRDEIFGLEKASLLDR
                     TAACEAKGVRWTLHSDEPVTEMGPLRCIENAVTRRLWRTDDEVLAPHECVGVEQALRA
                     MTRDAAWQCHSDHEIGTLEPGKLADLVVLEEDPRSVPPSRLGAVRVLETWVGGERVYQ
                     A"
BASE COUNT         2213 a         3688 c         3555 g         1633 t
ORIGIN      
        1 ctcggggcca tcctcgccgc gttgcgatcg gccgagcgca cgggcgaggg ccaggtggtg
       61 gaaacttctc tgttcgagac ggccatctgg acccaggcct cggatttcgg caccacggcc
      121 atcgaccgtg cccccgtgcg tcggcgtgcg cgtcacaaca tgctctcccc gaccgcaaac
      181 cgctatccgt gcggcgacgg caagtggctg gtggtgaaca tgctggatgc catggcgttc
      241 gaccgcttct gccgggcaat cggcagagaa gactggctgg aggacgagcg tttccgtgaa
      301 gcccgcggcc gctacgacca catgcccgag ctggttacgg gcatagatgc gaccttggcg
      361 acccgcagcc gcgatgagtg gggccggatc ttcgacgaag ccggcctgat ctgggggccg
      421 gtgctgaccc tcgacgaggc cgccggcgac ggccaggctc acgccatcgg catgttcccc
      481 gagctcgagc acccggagta cggcacctac cggacggtca acgcgccgtt ccgattccga
      541 tcgggggagg tcaggccgag aggaacgtca ccgggtcccg gtgagcacac cagagaagtg
      601 ctcggcgaga tggatttcga cgaggcggag atcgacgcgt tgatggcggc cggggtgttg
      661 ccgacgcacg actagtccgc agaggcggct tcctcgcggg tgaagaagac gtcgtcgcgg
      721 acgaacccgg cggtgatgct cggtttgagc gccagaatgg caccgtcgct cgcaaatgcg
      781 accgcccatc gacgagggag cccgacgatg aggtgcgcgg gcgcgaccct gccgtcgtcg
      841 aagcgcgcca gctccacctg tccggtttcc tcgtcgcgga aggccgggag gaaacgcatc
      901 gagcggttgt tctggctgac accgttggtg ccggagaagg cctggttctg ctggcgcagc
      961 gacctttggg tgaccggatg gttcatgacg acgtgaagct cctacaactt ccctgaagct
     1021 ccgcacatgc cagtcgagac ctcagctgga atggcagttg cgtttcagat tcgctcattc
     1081 gttacctacc gtttcgttta gcatcaacca tgccaaactc cagaatttca gccgaggctc
     1141 cataacaccc tgttttccat gaacaaatcg tgaacgaaac gccgtagcag gacactttcg
     1201 tgtgacgccg cttgtcagtt gcgcgcccgg ctgccccacc gcgcccgggg cgcgatggcg
     1261 tgccgcctgc tcgagttcac atgaacggcc cgaaccagtt ccatccgacg ccagctcgcg
     1321 cattcggggc aacgggctaa agtcatcgtt ccgcgccaca tgcggcgtgg gtctgaacag
     1381 cagatacggt gggccgacct ccgaagatgg agatcgatca tggctgcttc ggaaccgcga
     1441 agatgccttg cgcgacatgc ttgcgcgaca tggtggaaac cggcttctag ccgacgacgt
     1501 acgtttccgc ccgatctccc cagcttcccc ttgactggca ggccgtcccg ggcacgacgc
     1561 cggattccta accggccgcc gccgcttccc cgtagttcct ccgattggga aggtagcggc
     1621 ggagaaaccg ctgcaaagtg ccggttgccc cccagtagat gagctgaacg agaatgatca
     1681 aaccgacgaa gaggggaagg tagcccagat ccgggccacc cacgcggaag aagaacaccg
     1741 cgttgtagat cttctcctgc gtcgggtggc ttgccgtgac gatgccgatg tagccgcccg
     1801 cctggtcgaa ccgatgactg gcggtcagca ccccgtcggc ctgtttccgg ggcggctggt
     1861 agaagaccgt gcgcggcgtc agatcgccaa gggcatgcac gtcatcccag ttcgcgaacg
     1921 tgccgagctc cgtgtcatcg gagatgatgc ggaagtccac cggcatctcc ttcatgaagt
     1981 cgtgcatgta ctcgagcacg aacaggctct cccccacctc gggaatgtcc tcgcagaatt
     2041 cctcggatcc cctggattcg ggctggtagc cggtgaagtg ggcgagaagg aagtccatct
     2101 tcagaacgca ctggtcctcc tcgagcacta ccccgccatg acccgatgcg acctggggcg
     2161 ccagcagcag ggcgagcagc agcccgcttg ggagacgagg agcggccggt ctggtcattg
     2221 ggagcttgat gcgttcttcc tccggcgtct tgcctgatcg gctaactttg gcatacaaca
     2281 ggcaacgatc caacggtgac caaggggcgg cagacatggc cacgatcgcg attctcgggc
     2341 tcggcaactt cggcacggcc ctggcccggg tctggctgct aggcgggcac gacgtccgcg
     2401 gatggacggt ggaggaagac gtgttcgacg ccatccgctc caaaggcagc aacagcaaat
     2461 acctcgacgg catcgacctc accggcctgc aggtatccat ggagcttggc gagtcggtcc
     2521 ccggggcgga gctcgtggtg ctcgccctgc cctcccacgt ggttcttcac gtcgccgacg
     2581 acgtcatacc cctgctgacg ccggatcagg tggtgctgga tctcgccaag ggcatcgccg
     2641 gcgaggagct cgtttccgat gccatccagg ccaagctggc ggccgccggc aagaccaacc
     2701 cgctcgcggt gatcaccggc ccaaccatcg cgcccgagct tgccagcggc gtgctcacca
     2761 cggcgctcgt ggccagcgaa gacgaatcgc tggcgagccg cctcgccgag cagctatcca
     2821 cctcgacttt ttcgctgaag gcggccagcg acccgcgcgg cgcggagttg tgggggggct
     2881 tcaagaacgt gatcgcgctg ggctgcggaa tcgccgacgg gcttgacgag ggaagcggcg
     2941 acaacctgaa ggcggcgatc ttcacggccg gttttgccga aggtaatcgc ctgctcaccg
     3001 cgctgggggc cgcgccggag acggcgttta ctccggccgg catcggcgat cttttcgtca
     3061 ccgcatcgtc accccacggc cgcaaccggt ccctgggcca gaagctgggc gccgggcaga
     3121 ccctggagga agccatggcc ggcagcgtca tggtcgcgga gggcgttcgc gccacgcgaa
     3181 tgtttgccga gctcgccgca gcccggagcg tgaccacgcc gttcatcgca gcgcttacgg
     3241 acctcctcga cggaaactgc accgcgcccg actgcgtccg ccgactgatg gcgatctgaa
     3301 gcagacggcc cctctcggca acggccaatt gtggctgcgg ggtaatcggg taccatagcc
     3361 ggtctcatca catcgcaaag gggaggagcc aaacatggcc gcattcttcg tcgctcagta
     3421 caccgtcaac aacccggccc tttacgccga gtaccagcag ggcgccgggc ctactctggc
     3481 cacctacggg gctgagctgg tctctttcga cgtaggcgcc gagaccgtcg agggcaaccc
     3541 gcccggcccg cagacggtga tcatcaagtt cgaatccacg gacgccgcca aagcctggta
     3601 caactcggct gagtatcagg ccgtggtcgg caagcgcctg gaagccaccg acggctttgc
     3661 cgtcatcgct cagtcgatga acgcgggcgg ctgatcgcgc accgcacggc gcggggttga
     3721 cggccgctca gccgtccagc tcaccgcgcc gtgccagatc cctctccagg tcccgacccg
     3781 ccatccagta gtacacggtg gccacgacgt tgagcagcag cacgcagatc agcgcgtagc
     3841 gcagcgagtc ggtgccgagg tccccgaagg cgttgaaccc atcgctgagg atgcccgtga
     3901 cctggggacc cagcccaaga ccgatcaggt tcagaatgaa cagcagtatg ctcgaggcca
     3961 gcgccctcat gcgcagccct accagtccct gggtcagcgc gaaagtgggg ccgagatagt
     4021 aggctcccag caacgtcggc acgatggcca ccaggaacgc cacgcggtag tcgtcgtaga
     4081 ggtaaacgac catcgagaac ggcacggaaa gcagcgtcgc gatccccggc agccacacgt
     4141 accagcggac gtcccgcctg gccatgcggt cgcccatgta gccaccgaga aaggtgcccg
     4201 ccatgcccgc aaagccgagg tagaacagcc acgttccgat ctctccggtg ccgaggccgt
     4261 ggctgcgtat gaagaacgcc gggaaccagt agcccacgcc gtacccgacg aaggcgtgga
     4321 gcgcgccggc cagcgacagg tagcgaaagc tcttgaggga ccagaggtag cgaaacacgg
     4381 tcatgaccgg cggcgcggct tccgagctcg ccgtcatgtt gtccgcagcg cctcgcaccg
     4441 gctcgcgcac cgtcagaacc acgagaaaag ccagcgccac cccggggagc cctaccacga
     4501 tgaaggccgt tcgccagtcg aaagcctcgt tgatccagcc gccggccagg ttgccgatca
     4561 tcgtgcctac gggaattccc aaagcgtaga tggagagcgc cgtggcgcga gacgactcgg
     4621 gaaacatgtc tgagatcatg gagtgcgagg gcgggctgcc ccccgcctcc cccaccgcca
     4681 cgccgatgcg tgctagcagc aggtgggcga agctcgaagc gagcccgcag agggccgtca
     4741 tcacgctcca gatggccagg cagacggcca gcaggttgcg ccgcacggtc ctgtcggcca
     4801 ggcgggcgat gggtatgccc agaaacgtgt aaaagaccgc gaaagcgatc ccgcccagaa
     4861 agcccatctg ggtgtcggac agagccagat cctctttgat ggcctgattg aggatcccga
     4921 ggatcgaccg gtcgatgaaa ttgaagacgt agaccacgaa cagcacgccc agggcatacc
     4981 aggaacgcca gggcgtcttg ccggagaaac cctgcgcggc aggtgccccc gccggctgct
     5041 cctccacctg atcagtcatg gtttcctgcg agaccctgtt ccagcagctg ccggtcgtgg
     5101 tagcgcgcaa gcaggacgag cgccagcacg gcgccgagca acgcccaccc catgtcggac
     5161 tgggtatccc acacgtaacc ctgggtgccg agaaacgact ctgcggcctg ctcgctcaag
     5221 agcgcgaccc accactcgat cagctcgtag aaggcgctga tcccaaggca cagcgagacg
     5281 acgaaaaggt tgagccagcc gccacgctcc aggggggaaa gcctaagcag aacctcccgc
     5341 gcgacgatgg cgggcacgaa cccctgagcc aggtgcccga gcttgtcgta gttgttgcgc
     5401 gtgaatccca gcggttcccg caggagatcg aacaggggca cctcggcgta cgtgtagtgc
     5461 cctcccacga tgagtatcag cgcatggacg agaatcagcc cgtacgtcag ccgggtgagg
     5521 ggaaagcgcc gccgcgtgag aaagatgatg agcgcccccg cgaccgccgg cgagacctcc
     5581 aggacccata cctcgtaatc cttcgggttc acagccgacc agagcagaac cgccagaaac
     5641 ggggccgccc agagcacgat gccccgctgc gatgggtagt cggccaacta caacctcata
     5701 cgagtgagct agaatccgca agatacaaga gaaacggccc ggcggtaagc cgggcgccag
     5761 ccaggatggg aggtgaccag gtgaaagttg ctcaggccat tgccaaagcg ctcaaggccg
     5821 agggcgtgga ggtgctgttc gcatatccgg tgaacccgat cgtcgaggcg gcggcggcgg
     5881 aggacatccg cacggtcatc gtccgtcagg agcgggtggg gctgcacatg gcggacgcct
     5941 actcacgcct gtcgtcagga gacaggatcg gcgtgttcgc gatgcagcac gggcccggcg
     6001 ccgagaacgc ctttggcggc gtcgcccagg cctactcgga atcggtgccg atcctggtgc
     6061 tacccgcggg ctatcaccgc cgcatcgcca actactatcc caatttcagc tcgacgctga
     6121 acatgcgcaa catcaccaag tgggccgagc ccctgaccat gggcaaagcg gtccccgagg
     6181 tgatgcgccg cgccttcttc cagctgcgca acggaaggcc cggccccgtg ctcgtcgaaa
     6241 ttcccgtgga cgtgttccgg gaggacgtac ccgatgactg gcaccacacg ccgagcttca
     6301 aaacccgcag cgcgccggac cccgacgccg tcgccacggc agcggaggtg ctggccaagg
     6361 ccgaaaggcc cgtcatctac gcaggtcagg gcgttcacta cgccaaggcc tgggcggagc
     6421 tcaaagcgct cgccgagacc tggaacattc cggtaaccac cagcatcgag ggcaagagcg
     6481 cgtttcccga gaaccacccc ctctcgctgg gctccggcgg cagggccaac ccgcggcccg
     6541 tgaaagcgtt tctcaacgaa gccgacgtga ttctgggaat cggctgcagc ttcgccctca
     6601 cgggcttcgg cgtgccgatg cccgcgggca gaaccatcat tcacgccacc ctggacccca
     6661 tggatctcaa caaggacgtg cccgcgcagc acgggctcat cggcgacgcc gcgttgaccc
     6721 tgcgcgcgct cagccaggcc atggacggca aggaccgcag ccccgcggaa gcgcgcgccg
     6781 gcgtgagcgg gcgcattgcg gccatacggg aggagtggct gggcgagtgg atgccgaagc
     6841 tcaccaacga cgaggccccc atcaacccct accgggtgct gtgggagctc aaccagatgg
     6901 tggacaaaga caacacggtg atcacccacg acgcgggcag ccccagagac cagaccacgc
     6961 ccttctggga atgtacgacc ccgctgagct atatcggatg gggcaagacc acccagctcg
     7021 gctacggcct tggcctggct atgggagcca agctcgcgtg ccccgaaaag ctgtgcatca
     7081 acgtctgggg cgacgcggcc atcggcttca ccggcatgga cttcgaaacc gcggtgcgcg
     7141 aacggattcc catactctcc atactgttca acaacttctc catggccatc gagattccca
     7201 tcatgcagat cagccaggcg aagtacggcg ccaccgacat cagcggcaac tacgcggaca
     7261 tggccaaggc cttcggcggc tacggcgagc ggattaccga tcccggggac atcagggctg
     7321 ccctggaacg cggcatcgag gcgacccgag caggcagccc ggcgctgctc gagttcatca
     7381 cagacaagga aatcaccgtc tccaacgagt aggcgcgtcg cagcccgggt caatccgcgg
     7441 cgtgcatgag cggctcttcg tcgaggtcgc tgccgcgttg gcgctcgacc agccaaagcc
     7501 gcgacgtggt gaagatcagc agctgcacca cggccatccc gatgacgggc acgagcacgg
     7561 cgttccgggc gccgagcaca cccacgcacc agccgaggaa agccgatccg aacggcatgc
     7621 cgcccatgag cccgagggaa tagaccgaca tgagccgggc gcggtgactt ggaggagacg
     7681 cttcctgaac gatggaccgg gacatggtca tgctgacccc gccgcaggtg ccccacagat
     7741 agatgacggc atagaaaccc caaagcggca gctcgaagtg cagcaacgaa agcacgcaga
     7801 cgccgaagac gccccacagg cccagcgcgc gtccgggacg atcgatgcca ccgcgccgtc
     7861 gcaggaagaa caccaccgtg cacgtgccga gcatgttcgc cgcaaacgcc ccggcgatgc
     7921 ctccggcgcc gcctccgtac agatcgcgga ccatcagcgg gagaatcacg aggtaggcgc
     7981 ccgcgaagaa gacgccctgg gcgaaggtga gcagaacggc cggccggatc gcctccgagc
     8041 gccaggccat gacgatgccc tccccgatct cgcgcagcgc cgggcgccgc tcccgagcac
     8101 tccccatggg caggagcggt attttcgagg ttgccaccgc cgccgcgccc atcagcagcg
     8161 cctggacgag gaagagccag agcgggccca gcgaatcggc cgtcgagcca agggcgaacc
     8221 cgatgatctg cacgccgaac tgaaccccga tggccagcgt caccacaccc tgaacgtcgc
     8281 cgcccgccac gcggttgagc agagcatcgc gcgcgggctg cgcaaacgct ccgaacgaac
     8341 cgccgatcac ggcccagccg agcagcacct cgtagacgat gagatcggcc tgaaccagca
     8401 ccgccatcgc cagagggggc ccgatcatgc ccaggtgcag gccgaccaga aggcgccgct
     8461 ggtcgaagcg gtcgccaacg agcccgcccc acagaatcag caacagcatc ggcagctgca
     8521 gcgacatctg ggcgatgccg acccgggccg gcgattcctg caggtacacg gccacgagcc
     8581 acgggaacag gaccatctga atgccaccgg gcacgagaaa caacccggtg ctgagcagat
     8641 accaccgcag gccgcgatcc atgggtcagg cagccgccat cattcttttc ctgaccatgc
     8701 gaacggcgac gaggcccacc accagggtgg ccgcgtagac ggcacagatg aaggtcagat
     8761 cgtagctcgg cagaatcggc gccatgaagc cgagcacatg aaagctcagc accaagatgg
     8821 cgatgttgaa gaacgcgagc tccttggcga tgcccgtgct caggtccgga tcgacgattc
     8881 tgagcagcag cagcccactt cccgtggatc cggagcaaca gccgaacgag gtcagcgagc
     8941 gttcagccga aaggttgggc aggtgcctgc caagaccaaa acacaacacc acggttacgg
     9001 cagacacggc aaggcagacc acgaggatcg gcacgatgaa ctcggacagc agcccaaaat
     9061 gaatgctcat gatggtggcg acgaccatga agtccaccga cgtgccggtt atccgccgct
     9121 gggtctcgtc atcgatgtag tggcccaggc cgaaacggtc cagcagcgat cgcatgatca
     9181 cgcagaccat caggccgtgg acgaagaaaa ggttgtggct gaagagaaca ccgaagttca
     9241 catccccgat ggcccacccg tcggcaatcg gccgcatgaa gctcaagtac tggtcggtca
     9301 cgagataggc cacgccgaga acggcgatgt ggaacgccag ggtatcgatg ttggcggagt
     9361 gggtgatctg tcggcctgcg gagacatcgg agtcggcgtt cagaatgcct gcctgaaact
     9421 cgtcgtctat ccgagcgacc cgattcacgt tgcgtcctgc cttgatggcg tggcgcgcca
     9481 cggggacgcc cacggcgaaa gcgacgatga agccggccga tgcgtagatg atgccgaagt
     9541 tgatggcatg gaccacgccc agctccgact cccagatcga ggcgtaagcg agcgcctgac
     9601 caggcccctg ggtgaagccg tgggttgcca gcaggccgag gaagctcgat agctcgccgc
     9661 ccgtcagcac gttgtagcca accagcgcgc cgaggccaac gagaccctgc gtgaccaggc
     9721 tgaccaccca gaccaccgac atccacatgc cgccagggat gatggaggtg ttcttgccgc
     9781 gctctctgct gccgcccgtc aggcagagcg acatgaagct gagggtgaag aaatggaagg
     9841 taaacaccgt gaagtcactg ctctcgtagc cgaaggaaag tcccgccgag acgagagcga
     9901 agccgaggat gccccctacc aggctggccg gcacgagcgc tctgcgcaga agcggcacct
     9961 tcgcgcggag cacggttccg gccagcagca tgcacaccat gaaggcaaag gcgatcaccc
    10021 ccggaaacgt cgaagtcatg ttgaaaccct tctgttgtga aacgtcgccc gaggctgcgc
    10081 gtcaggcctg atagacccgt tcgccgccaa cccaggtctc gagcacccga acggcgccga
    10141 ggcgagacgg cggaacgctg cggggatcct cttccagcac cacgagatcc gccagcttgc
    10201 ccggctccag ggtgccgatc tcgtgatccg agtgacactg ccaggccgca tcccgggtca
    10261 tcgcccgcag cgcctgctcc acgcccacgc attcatgggg ggcgagcacc tcgtcgtcgg
    10321 tccgccacag ccggcgggtc acggcgttct cgatgcagcg cagcggaccc atctcggtga
    10381 cgggctcgtc ggagtgcagg gtccagcgca cgcccttggc ctcgcaggcc gcggtccggt
    10441 ccaggagact ggctttctcc agcccgaata tctcgtctcg aaaggccttg ccccaccagt
    10501 agacgtggcc gatcagaaag ctgggcgaga gcccgaggtc ggcgatccgc tcgatctgct
    10561 cgtcgtggag gatgctgcag tgctctatgc ggcagcgcgc tcgcgccgga tcgagccccg
    10621 cggcccgggc ggcctcgaag gcatccagca ccctgtcgat ggcaagatcg ccattggcat
    10681 gcacggcaac cggccagccc tgcccgagtc ttcgtttcac ggcctcgttg agcgcctcgg
    10741 gttcgatgta ggcgatgccg tgggagtcgg cgttcatgaa cggctcccgc tgcagcccgc
    10801 tcaacccctg attggaaccg tcgctgacga tcttccaggc gctggcgcga acccagctgt
    10861 ctcccgtgcc gaacgagacg tcggaggcgt cccagtcggc ggcaagggta ttgaacaggg
    10921 agtaacgaaa gcgcgccgtc atccggttgc tttcgcgcag cgcttcgtaa agagcgagct
    10981 ccttggcgcc ctgcatcgcg ccggtgccct gatcgcagat ggtggtgaag ccgacgctgt
    11041 tggcatggcg gaacacgtcc aggcacccct cgaccagatc gtgctcgcg
//
LOCUS       DBNHLM010000168        20866 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_323261, whole genome shotgun sequence.
ACCESSION   DBNHLM010000168 DBNHLM010000000
VERSION     DBNHLM010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 20866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20866
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_323261"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="AC16QF_07655"
     CDS             complement(<1..880)
                     /locus_tag="AC16QF_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6448470.1"
                     /translation="MRISMLLSYAGGFKEAVKEVAELEKAGLDLVWVPEAYSFDAPSA
                     MGYLAANTERVTIASGILPIYSRTPSLLAMTAAGIDYLSDGRCMLGLGASGPQVIEGF
                     HGVPYVAPVSRIREIIEICRKVWRRERLQHDGRYYQIPLPQNKGTGLGKPLKLINHPV
                     REEIPIAIAALGAKSVEMTAELADAWLPAFYTADAADAVWGDALRAGAAKRDPGRAPL
                     EVFAGGAVAIGEGLEDLRNLARPQAALYIGGMGARQKNFYNDIFSRSGYAQEAKLIQD
                     LYLSGDKKGAEAAIPES"
     gene            1005..2222
                     /locus_tag="AC16QF_07660"
     CDS             1005..2222
                     /locus_tag="AC16QF_07660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR6448471.1"
                     /translation="MDREFDVIVWGATGFTGRLVAEYLNERYGLGGSVAWAIAGRNQG
                     KLDAVRQEIGAPGLSTILADGNDEASLAAMAERARVICTTVGPYALYGSRLVAACVSR
                     GTHYCDLTGEVHWMRRMIDTHHLSAQQAGVRIVHTCGFDSIPSDIGVLCVQREMKSRH
                     GEVATEVKSRVRGMRGGFSGGTVASMLNMMEESEMDPGVREVMNDPYGLNPAGERHGL
                     DGPEHSVPEYDPDFGAWTTPFVMAAINTKVVRRSNALSGYAYGRDFRYDEAMLTGFGP
                     WGFPLAAAMSVGSTAFNAAAGVSALRGLLARVLPEPGEGPSAAARERGFFDIEMLGKS
                     RRSGARVKVRIRGDRDPGYGSTSRMLGESAVCLARDDLETPTGVLTPAVAMGEALIGR
                     LGNNAGVTFEVMP"
     gene            2266..3345
                     /locus_tag="AC16QF_07665"
     CDS             2266..3345
                     /locus_tag="AC16QF_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448472.1"
                     /translation="MSETGPAPQERGKSNILLRIATWLITAGCFYLVYGRTAEAAARD
                     GLTVLEYLARFFGEANWLAWLAVMVPYSVFFFVVDAHVTWRVIRWFNAPEIRLGNVLP
                     IRASAYILSLVNEQVGKGAMSLYLYRRYKVPALAAISSMLLLGLMEIYQLLVFSSVGV
                     FLGFEVVQAASTELPLDKILPGVFAVAAIYLPLHIAYFRGHLLKGSTVREKSLVRAFR
                     EARPIHYLLILLFKAPLLIGAIVVYTIALNLFNVDVSFAQMLAFLPVIFLAAALPLPF
                     HAGALLFWTILFPDFPEVGAFSLVMHTFFVLFNALIGVVFLPIANRELFGSEQPGSGT
                     VAHGNDAGDDEHPGQPLDQGGAGPR"
     gene            complement(3235..3762)
                     /locus_tag="AC16QF_07670"
     CDS             complement(3235..3762)
                     /locus_tag="AC16QF_07670"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR6448473.1"
                     /translation="MWFTWANLLTTVRLALAVPCAMAVVSGDWPLAAALFALAVITDL
                     LDGPLARRLNHASPVGGLFDHATDALFVTFALGALATAGYLTWALPCLVAAAFIQYAL
                     DSRALAGKHLRTSYLGRMNGIAYFFAAGIPIVKEGLALPWPSAALIQGLAWVLVVTSI
                     VSMGDRARAWLLRSE"
     gene            3906..4805
                     /locus_tag="AC16QF_07675"
     CDS             3906..4805
                     /locus_tag="AC16QF_07675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6448474.1"
                     /translation="MIVVTGANGNLGRRLLQSLSGRAPLRAVVRSERAAEQIRALTLE
                     PEPEICVVDYQDAAAMTGALAGAEYVVHLVGIIKETAASTYVDAHERTSEVLCQAADS
                     AGALRIVYLSIVGSEPGSSNPCLASKGAAEAILLAAATPALVVRVPMVLGEGDYAAGA
                     LARRARKGWNVLLRGASLEQPIYAGDVIEAVKAGLGSAPAPAALDDAVLDLAGPVSLS
                     RSDLTRRSASILGRSTRVISLPLFLGLLLAGLLERLLANPPVTAAMLRVLDHDDRLDP
                     EPACHVLGIELTPLDEVLARCLR"
     gene            4938..6629
                     /locus_tag="AC16QF_07680"
     CDS             4938..6629
                     /locus_tag="AC16QF_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448475.1"
                     /translation="MQELSNSGLTRSADNAPEETGSGPARAPGKGRELDDLKQELAKW
                     QERVPKLAAALRERTEHASELQAQIEKLEANHRASSDQAAAASKERQQRVDELHSQLQ
                     AQQAKHQELQSELQARGAQIADLRLEVADWKEKHQHLVEAGEQASGPSEEDQAALEAL
                     KRENASLAEALAEKTRLAEERAREVAALREDAESLSSRNATLFETTEFASKQLDSLGD
                     NLGELRDELNARSEALREKTARIEALEGQLSERETELEVFREKMESAGVYEEMALELE
                     ESLAAERQEVERLQQCVKTANRITSEREYERRALSEELETLMERNQELEAAAEAGGDQ
                     DPELEQQRDLMLSLEEELAQAKDEQVALEKSHAKTLAEKERQLSELREELDAAADRAG
                     KLAADESIGEAQPEHPAQDKKVEVLQQQVTTLEELIRERTEELNELKWRAQLADGERE
                     AITEDDAKMMLVLNQQLGEARESNDRLIAVIGRLEERLAASDLGPAGDDFTRMRGIGD
                     KLAAQLNELGIRTFGQIADIDLAALENDHHPLAAFKHRIEKDDWVAQARELAGRG"
     gene            6767..7423
                     /locus_tag="AC16QF_07685"
     CDS             6767..7423
                     /locus_tag="AC16QF_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6448476.1"
                     /translation="MSHSSVPLVLRRTTSFLFMVVLVPMGLGVGSAVADVAAGKAAYM
                     VCASCHGQNGEGNAALNAPRIAGQEPWYISRQLAAYRAGHRGTAPGDIYGMQMRPMAM
                     TSADPATEANLIEYIGTLSAAPATATIEGDAAAGQNGYAVCVACHGANGEGNQALGGP
                     KLAGQNDWYLVRQIKNYQSGLRGYDPADTYGMQMKPMAGVLADDKAINDVVAYINTLR
                     "
     gene            7426..8472
                     /gene="alr"
                     /locus_tag="AC16QF_07690"
     CDS             7426..8472
                     /gene="alr"
                     /locus_tag="AC16QF_07690"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR6448477.1"
                     /translation="MRGRLHVDLAALAANYRSFSSAAEAAGGTAGGVVKADAYGLGAA
                     RVAATLQSEGCAEFFVATVEEGAALRAILPEAIIYVFEGATGATAEVLASNDLVPVLN
                     HRGQLEAWQAYRERPAAVHVDTGMNRLGFPADVDAVQFAGMNLSLLLTHLACADVPDD
                     PMNHEQVERFRRAATSFPGVRTSIGNSAGMLADASCCGDLGRPGVGLYGGNPFSDRRN
                     PMRRVAALQGQVLQVRTCSAGSPLGYGATYVAPADLRVAVVGLGYADGLPRLISGRGC
                     AAVGGRRCALLGRVSMDLTLVDVTGLEVQAGDWIEMFGDTVSVDEVAGWAETIAYEIL
                     TGIGDRVERVYSGD"
     gene            complement(8457..9068)
                     /locus_tag="AC16QF_07695"
     CDS             complement(8457..9068)
                     /locus_tag="AC16QF_07695"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6448478.1"
                     /translation="MAETAVDAGVERGGEAGGAPGSIQSAAELLVADEAALGCHREVT
                     AADPNPEPCDVFLRLTADSLDETLRQAAAHNNRGLILTRLGGLEAALADFEAALALAP
                     GLGAAEVNRGTALLHMGLYPDALKAFENVIVNDEEHRATARFNRSFVYRALGELDLAA
                     ADLASAAQTDLPVSRSREPIPLQAGPAAEPLDRPMELEGQSPE"
     gene            9263..10648
                     /locus_tag="AC16QF_07700"
     CDS             9263..10648
                     /locus_tag="AC16QF_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HIR6448479.1"
                     /translation="MADPLHNQFPPGSLEARDLEHLLHPTTNLEQHHKVGPRVHDRAE
                     GVYLWDSQGRQYLEGMAGLWCTALGYGEEELVSAAAQQMRKLSYSQLFGGRSNEPSIL
                     LAERLKAMAPFDAGRVFFGLSGSDANDTQIKLMWYYHNAIGKPEKKKIISRMRGYHGV
                     TVAAGSLTGLPAFHAHFDLPIPGVLHVDAPHYYREAKGGETETEFVDRLVGDLEELIL
                     GEGPETIAAFIAEPVQAAGGVIVPPPGYFAKVQALLARHDILFIDDEVICGFGRTGRA
                     FGAQTFGIEPTTMSVAKGVSSAYLPLSAVIIPEFMYEPLVELSGSVGTFGHGFTYSGH
                     PVCAAVALRTLELMEEREIFAHAAAVSEPFQERLALLADHPLVGDARGVGLLGAVELV
                     ADKDTRTPFPPGQGVGAHCQERCESHGLILRALGDVVAICPPLIISEDQVDELFAKLE
                     RALDDTFAWVH"
     gene            10739..12349
                     /locus_tag="AC16QF_07705"
     CDS             10739..12349
                     /locus_tag="AC16QF_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017709070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR6448480.1"
                     /translation="MAILQSNIDTGAAAFAANAKHHRELADELRTLAGRIVEGGDARS
                     RERHVGRGKLLPRDRLSRLLDPGSPFLEVGQLGGYELYDDWIPSGGIVTGIGRVSGTQ
                     CMVVVNDATVKGGTYYPITVKKHLRAQEIALENGLPCVYLVDSGGAFLPMQAEVFPDR
                     DHFGRIFYNQANLSARGIPQIAVVMGSCTAGGAYVPAMCDQAIIVRGQGTIFLGGPPL
                     VKAATGEEVDAESLGGGDVHSRTSGVTDYLAADDVHALGIAREIVAASQQRYRATVET
                     RTPRPPAFAEEELYGVLPTDPRTPVDVREIIARLVDGSEFHEFKALYGETLVCGFAHI
                     EGMPVAVIANNGILFSESALKGAHFIQLADRRGVPLLFLQNITGFMVGKKYENQGIAK
                     DGAKMVTAVACARVPKFTVVIGGSFGAGNYAMCGRAYGARMLWTWPNARISVMGGEQA
                     AHVLATVRRDGAAARGEEWPEAEQAAFMDDIRAQYETQGHPYFASARLWDDGVVDPAD
                     TRRVLGLALEVACREPLQPQSGFGVFRM"
     gene            12352..14280
                     /locus_tag="AC16QF_07710"
     CDS             12352..14280
                     /locus_tag="AC16QF_07710"
                     /EC_number="6.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HIR6448481.1"
                     /translation="MFDTLLIANRGEIACRVMRTATRMGLRTVAVYSDADAHAQHVRL
                     ADAAERIGPAPARESYLRIDAVLDAAERHGAGAVHPGYGFLSENAEFAAACEERGIVF
                     VGPSARAIRAMGSKIEAKRIVAAAGTPVVPGYQEADQSDAALSRAAAEIGYPLLIKAS
                     AGGGGKGMRLVREPDEFPAALAGARREAAAAFADELVLLERYLDAPKHLEVQILADQT
                     GHTLHLYERDCSVQRRHQKVIEEAPGPTVSEELRTLLGEAAVQAAKAIGYVGAGTVEF
                     ISQAGEFYFMEMNTRLQVEHPVTEAITGLDLVEWQLRVAAGEKLPFRQHEIPRNGHAV
                     EARVYAENPARRFLPSTGTLAHVAFPQEVRVDAGVATGDAVSVHYDPMLAKVIAHGPD
                     RDAAITSLERALARTEIAGVAHNVAYLRRLLAHPVFRGGDYTTGIAEEHADELLAEED
                     HLPAICAAIAAGRLGDSVGPWARRDGFRLNGPASFIQRFRRERKEVEVVLSEGQAQVD
                     GEVFDVADVSWNEGVLALRVDGNRVGARVVTAGPSLYVIIGGSTARFDPGDVEGAARS
                     LGSAAGNRVVSPMPGQVIAVSVKPGDRVKAGAVLAVVEAMKMEHSVAAPRAGRVASVN
                     CRVGDRVDEGVELVVLEE"
     gene            14303..15466
                     /locus_tag="AC16QF_07715"
     CDS             14303..15466
                     /locus_tag="AC16QF_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009686373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HIR6448482.1"
                     /translation="MSAAEFDFGFDETLNMLRDSVRQFAADEIAPRAAQIDASNEFPR
                     DLWPKLGALGLLGVTVEEAYGGAGLGYLAHCIAMEEISRASASVGLSYGAHSNLCVNQ
                     IRRYATEEQKQRYLPPLISGDYLGALAMSEPGAGSDVVSMRLRAEDRGDSFVLNGSKM
                     WITNGPGADVLVVYATVDPSLGPRGITAFLIDKGTPGFSTAQKLDKLGMRGSDTCELV
                     FDDCEVSKTQVLGEVGQGVRILMSGLDYERVVLAAGPLGIMQSCLDTVLPYVHEREQF
                     GQPVGTFQLMQGKLADMYTTMNACRAYVYAVAQACDAGRTTRFDAAGCILFAAEKATW
                     MASEAVQALGGNGYINDYPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFDATG"
     gene            15579..16037
                     /locus_tag="AC16QF_07720"
     CDS             15579..16037
                     /locus_tag="AC16QF_07720"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6448483.1"
                     /translation="MNFLLIGLALSGVALVAHAVPPGSEDEIRARTAPMGALCRAGEN
                     CSGFGAPAAMSAPAVAGAQAPAGAPAAAAQSGEQIYNQFCVACHAVGVANAPKLGDVA
                     AWADRIAKGMDVLMASTLNGINAMPAKGTCMTCTDADLQAAVEYMVDGSR"
     gene            complement(16042..17592)
                     /locus_tag="AC16QF_07725"
     CDS             complement(16042..17592)
                     /locus_tag="AC16QF_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR6448484.1"
                     /translation="MNDPDGHTLKSATEKSLTRTRAQAGMDTPAVTVETHLPGGLPAF
                     TLVGLPETAVRESRDRVKSAIDNCGLSFPNGKVVVNLAPADLAKEGARLDLAIAVSIL
                     AATGQLPHRVLEGFEFLGELGLSGELRPIRGCLCAALASATEVHERRLVLPSANAGEL
                     AMAPPGRVVPLAHLREVVRLLREPGYRPAAAPSAKPAANAERESRSLAEVIGQPGAKR
                     ALAVAAAGGHHLLMTGPPGTGKTMLARRLAGLLPALTEAEAREAALVYSAAGLPLEYP
                     FVPPFRDPHHTASGPALVGGGGHPQPGEISLAHRGVLFLDELPLFKPSVLEVLREPLE
                     TRSITIARARYRARFPASFQLVAAMNPCPAGRVCSESACRCTPEQVRRYRARISGPLL
                     DRIDLHVAVPPIPKALLLAGARDSSSGVDQTKAAIAGARRVQLSRQGRLNVDLTGADA
                     TRLTRLNTESRRLLANAVDRFQLSARGFHRVLKVARTVADIEGHVDVGPPHVAEALSY
                     RSVTWEGE"
     gene            complement(17603..17878)
                     /locus_tag="AC16QF_07730"
     CDS             complement(17603..17878)
                     /locus_tag="AC16QF_07730"
                     /inference="COORDINATES: protein motif:HMM:NF016280.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HIR6448485.1"
                     /translation="MAEHSARNHSPGNEDVVGTFIERLTEQLASLVPPDSLRLVLDQA
                     RSALQRGLAEVDLVPRHELDAHLAALDRLTETTRDLERRVAALEADS"
     gene            18252..19001
                     /locus_tag="AC16QF_07735"
     CDS             18252..19001
                     /locus_tag="AC16QF_07735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HIR6448486.1"
                     /translation="MKKIITPVVAGILLFAAGASAPAAAEGPEFSGNIAYLTDYRFRG
                     ISQGDRSQALQGGFDIELDNGLYAGTWASNVGFTECCGSAGLELNYYAGFAGAFSEEV
                     DYDVGFLWYNYPEDDADPDLDYYELYGSVSAFGGTFGAAFSPDYFAETGDFWYLYGDY
                     SFGVAENLSIDLHVGFNLFEDEESFAAFIGPEDGDAGDNYIDYSIGVSTSALGLDFGL
                     SFIGTDLKKSECFGGTKLCDDTVVLSVSKSL"
     gene            19130..19468
                     /gene="glnK"
                     /locus_tag="AC16QF_07740"
     CDS             19130..19468
                     /gene="glnK"
                     /locus_tag="AC16QF_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR6448487.1"
                     /translation="MKLVTAIIKPFKLDDVREALSDIGVQGMTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEIAIDDGLLEQCLETITKSANTGKVGDGKIFVSSLEDVVRI
                     RTGETGAEAV"
     gene            19522..20763
                     /locus_tag="AC16QF_07745"
     CDS             19522..20763
                     /locus_tag="AC16QF_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR6448488.1"
                     /translation="MTQTNYALDTFYFLVMGALVMFMAPGFAMLEAGLVRSKNTAEIL
                     LKNVALFGIACIMYLICGYYIMYVGAVEGGILPDFGLWIGAENTVDETLASGGDVYYS
                     ARSDYFFQVVFVATAMSIVSGAVAERMKLWAFLAFAVVMTGLIYPLQGMWTWGGGWIS
                     TAGYSDFAGSGIVHMCGAAAALAGVLLLGPRKGKYNKDGSVNALPGANLPLATLGMFI
                     LWFGWFGFNGGSELKTSNIDEANAVAQVFVNTNIAACGGLVAALLTAKMLFGKADLTM
                     ALNGALAGLVAITADPLSPIAQWAMIIGAVGGVLVVFSIVTLDKLKIDDPVGAISVHG
                     VVGIWGILAVVFNNGDATIGGQLIGIAGIFAWTFVAALVVWGILKATMGIRVSEEEEY
                     EGVDLSECGLEAYPEFAKVSS"
BASE COUNT         3492 a         6578 c         7128 g         3668 t
ORIGIN      
        1 agctctcggg gatagcggcc tcggcccctt tcttgtcccc ggacaggtac aggtcctgga
       61 tcagcttggc ctcttgtgcg tacccggagc ggctgaagat gtcgttgtag aaattcttct
      121 gtcgcgcgcc catgccgccg atgtaaagcg ctgcctgggg gcgcgccaga ttccgcaggt
      181 cttcgaggcc ctcgccgatg gccaccgcgc cgccggcgaa cacttccagc ggcgccctgc
      241 ccggatcccg cttcgcggct ccggcgcgaa gcgcatcgcc ccacaccgcg tccgcggcat
      301 cggcggtgta gaaggcggga agccaggcat cggcgagctc cgccgtcatc tccacgctct
      361 tggcgccgag tgcggcgatg gcgatgggaa tctcttcgcg gaccgggtgg ttgatgagct
      421 tcagcggctt gcccaggccg gtgcctttgt tctgcggcag cggaatctgg tagtagcgcc
      481 cgtcgtgctg cagacgctcg cggcgccaaa ccttccggca gatctcgatg atttcccgga
      541 ttcgggagac gggtgccacg tagggcacgc cgtgaaagcc ttcgatgacc tgaggcccag
      601 acgcgccgag cccgagcatg cagcggccgt ccgagaggta gtctatgcct gctgccgtca
      661 tggccagcag agacggtgtc cgggagtaga tgggcaggat gccggaggcg atggtgacgc
      721 gctcggtgtt tgccgccagg tagcccatgg cgctgggcgc gtcgaaggag tacgcctcgg
      781 gaacccagac cagatcgaga ccggctttct ccagctcggc gacttctttg accgcttcct
      841 tgaaaccgcc ggcatagctc agcagcatgg atatacgcat gttcaacccc ttgagaaggt
      901 gatggttcgt tggtcgccgg cccggccgcg gagtatgctt ggcggctgcc gcccctttgc
      961 caccggcgtg gcggtcgatg ttaacagaat gaggatcact gccgttggat cgggaatttg
     1021 acgtcatcgt ctggggggcc accggtttca ccgggcgcct cgtagccgag tacctcaacg
     1081 agcgctacgg attgggcgga agcgtcgcct gggcgatcgc cgggcgaaat cagggcaagc
     1141 tcgatgcggt gcgtcaggag atcggcgcgc cgggactgtc cacgatcctg gccgacggca
     1201 acgacgaggc atcgctcgcc gccatggccg agcgcgcccg cgtcatctgc acgacggtgg
     1261 ggccctatgc tctgtatggc agccggctcg ttgctgcgtg cgtcagccgg ggaacccact
     1321 actgcgatct gaccggcgag gtccactgga tgcgccgaat gatcgacacc catcacctca
     1381 gtgcccagca ggccggggtt cggatcgtcc acacgtgcgg cttcgacagc ataccgtcgg
     1441 acattggcgt gctctgcgtg caacgggaaa tgaaatcccg gcacggtgag gtggccaccg
     1501 aggtcaagtc ccgcgtgcgc ggaatgcgcg gcggcttcag cggcgggacc gtggccagca
     1561 tgctcaacat gatggaagaa agcgagatgg acccgggcgt tcgggaggta atgaacgatc
     1621 cgtatggcct caaccccgcg ggcgagcgtc acggcctcga tgggcccgag cactcggtgc
     1681 ccgaatacga cccggatttc ggcgcatgga ccacaccgtt cgtcatggca gccatcaaca
     1741 ccaaggtcgt tcgccgcagc aacgcgttgt ccggctacgc ctacgggcgc gactttcggt
     1801 acgacgaggc gatgctgacg ggcttcggcc cctggggctt cccgctggcc gcggcgatga
     1861 gcgtcggctc gacggctttc aacgccgccg ccggggtttc tgcgctgcgt ggattgctgg
     1921 cgcgggttct gcccgagccc ggggaggggc cctcggccgc tgcccgcgag cggggattct
     1981 tcgacatcga gatgctcggc aagagccgcc gcagcggtgc gcgggtcaag gtcaggatcc
     2041 gcggggatcg tgatccgggc tacggctcga cctcgcggat gctgggcgag agcgccgtct
     2101 gcctggccag agacgatctg gagacaccca cgggcgtgct taccccggcc gtggccatgg
     2161 gcgaagcgct gatcgggcgt cttggcaaca acgccggcgt gacgttcgag gtgatgccgt
     2221 gaggcgctca cttcgtcgct catctaacga aaggagcgcg tgggcgtgag cgagaccggg
     2281 ccggctcccc aggaacgcgg caaatccaac atcctccttc ggattgcgac ctggctgatt
     2341 actgccggct gcttctatct cgtttacggc cggacggccg aagcggcggc acgtgacggc
     2401 ctgaccgtgt tggaatacct ggcccgcttc ttcggcgagg ccaactggct tgcctggctc
     2461 gcggtcatgg tcccctactc cgttttcttc ttcgttgtcg acgctcacgt gacctggcga
     2521 gtcatccgct ggttcaatgc ccccgaaatc cggctgggca acgtgctgcc gatccgtgcc
     2581 agcgcctaca tcctgtccct ggtcaacgag caggtgggca agggcgcgat gtccctttac
     2641 ctctatcgtc gttacaaagt ccctgctctg gcggccatct ccagcatgct gctgctcggg
     2701 ctgatggaaa tctaccagtt gctggtgttc tcctcggtcg gggtctttct cggattcgag
     2761 gtggtccagg cggcctccac ggagctgccg ctcgacaaga tcctgcccgg cgtgtttgcc
     2821 gtggcggcca tctatctgcc gctgcacatc gcctacttcc ggggacatct actgaaggga
     2881 tcgacggttc gggagaagag cctggtccgg gcattccgtg aagcccggcc cattcactac
     2941 ctgctcattt tgctgttcaa ggcgccgctt ctgatcggtg cgatcgtcgt ctacaccatc
     3001 gccttgaacc tcttcaacgt cgacgtcagc ttcgctcaga tgctcgcctt ccttcccgtg
     3061 atctttctgg ccgcggcgct gccgctgccg ttccatgccg gcgcccttct tttctggacc
     3121 attcttttcc cggactttcc cgaggtcggt gctttcagcc tcgtgatgca caccttcttc
     3181 gtgctgttca acgcgttgat cggggtggtg ttcctgccga tcgcgaatcg ggagctattc
     3241 ggatcggagc agccaggctc gggcacggtc gcccatggaa acgatgctgg tgacgacgag
     3301 cacccaggcc agcccctgga tcagggcggc gctgggccac ggtagggcca gcccctcttt
     3361 aacgatgggt atgccggcgg cgaagaagta ggcgatgccg ttcattcgcc cgagatagct
     3421 cgtccgcagg tgtttgcccg ccagcgcccg ggagtcgagg gcatactgta tgaaagccgc
     3481 agcaaccagg caggggagcg cccaggtcag gtagccggca gtggccaggg cgcccagagc
     3541 gaaggtgacg aagagcgcgt ccgtcgcgtg gtcgaacaag cccccgaccg ggctggcgtg
     3601 gttcaagcgt cgcgcgagcg ggccgtcgag caggtcggtg atcacggcaa gcgcgaacag
     3661 ggctgcggcc agcggccaat caccggagac aacggccatg gcgcacggca ccgcaagcgc
     3721 caggcggacg gtggtgagga gattcgccca ggtgaaccac atggcgcgat tatgctacgc
     3781 cagccaccca tcggtgtcgc cttgctgagc gcgttcgcgc gctccagaat gtggcccgtg
     3841 atgcgcgtcg aacaccagag cctgccggta tcgcccgccg atccatggat cggcaggggc
     3901 tgcccatgat cgtcgtcacc ggagcaaacg gcaatcttgg caggcggctt ctgcagagcc
     3961 tctccggccg ggccccgctg cgggccgtgg tgcgctcgga gcgggccgcc gagcagatca
     4021 gggctctgac gctggaaccc gaacctgaga tatgcgtggt cgattaccag gatgctgccg
     4081 ccatgaccgg ggcgctcgcg ggggcggaat atgtcgtcca tctcgtaggt atcatcaagg
     4141 agacggcggc cagcacctac gtcgatgctc atgagcgcac ctccgaggtg ctctgccagg
     4201 cggccgactc ggcgggcgcc ttgcggatcg tgtacctgag catcgtcggc tcggaaccgg
     4261 gcagcagcaa tccgtgtctg gcgtcgaagg gggctgcgga agccatcctc ttggccgccg
     4321 ccacgcccgc gctggtggtg agggtgccca tggtgctggg cgagggtgac tacgccgccg
     4381 gcgcgctggc gcggcgcgcg cgaaagggtt ggaacgttct gctcaggggc gcgagcctgg
     4441 agcagcccat ctacgccgga gacgtcatcg aggcggtgaa ggccgggctc ggttctgccc
     4501 ccgctccggc ggccctggac gatgccgtgc tggacctggc cgggcccgtt tccctctcgc
     4561 gcagcgatct tacgcgccgg tccgcgagca tcctgggtcg atccacccgg gtgatatccc
     4621 tgcccctgtt cttggggctg ctgcttgccg gtctgctgga gcgcctgctc gcgaacccgc
     4681 cggtcaccgc tgccatgctg cgggttctcg accatgacga ccgcttggat ccggagcccg
     4741 cctgccacgt cctcggcatc gagctcacgc cgctcgacga ggtcctcgcc cggtgtctgc
     4801 gatgacgaaa ccgtgacgcc cgtcacggcc atctgaattc ccacccagtt cacattcagg
     4861 ctgggcgaat tcaagtagtt ttgcgcggtg acccgagaaa gtcggccaga cggtcctcgg
     4921 agtggatgga tacccgcatg caggaattgt cgaacagcgg cctgactcgg tcagccgata
     4981 atgctccgga ggagaccggt tcggggccgg ctcgtgctcc agggaaggga cgcgagctgg
     5041 acgatctcaa gcaggaactc gccaagtggc aggagcgggt gcctaagctc gccgccgcgc
     5101 tgcgcgagcg cacggagcac gcaagcgaac tgcaggccca gatcgagaag ctcgaggcga
     5161 accaccgtgc ctcgtcggat caggctgccg ctgccagcaa agagcgtcag cagcgggtgg
     5221 acgagctgca cagccagctt caggctcaac aggcaaagca ccaggagctg cagagcgagc
     5281 tgcaggcgcg tggtgcgcag atcgccgacc tccggctgga ggtggccgac tggaaagaaa
     5341 aacaccagca cctcgtcgaa gcgggcgagc aggcgtccgg gccctcggaa gaggaccagg
     5401 cggcgctcga ggcgctcaag cgtgagaacg cgagcctcgc cgaagcgctg gccgagaaga
     5461 cgcggctcgc cgaagagcgc gcgcgggagg tcgccgcgct tcgcgaggac gccgaatcgc
     5521 tctcgagccg caacgcgacg ctcttcgaga ccaccgagtt tgccagcaag cagctcgaca
     5581 gcctcggcga caacctgggc gagctgcgcg acgagctcaa cgctcgcagc gaggcgcttc
     5641 gggaaaaaac cgctcgaatc gaggccctgg aaggacagct cagcgagcga gaaaccgagc
     5701 tcgaggtctt ccgggagaaa atggagtcag cgggcgttta cgaggaaatg gcgctggagc
     5761 tcgaagagtc gctggccgcc gagcgtcagg aagtcgagcg cctgcagcag tgcgtcaaga
     5821 cggccaaccg catcaccagc gagcgcgagt acgagcgtcg ggcgctgtcc gaggagctcg
     5881 agacgctcat ggaacgcaat caggaactgg aggctgcagc cgaagcgggc ggcgatcagg
     5941 atcctgagct ggagcagcag cgcgatctga tgctcagcct ggaggaagag cttgcccagg
     6001 cgaaagacga acaggtcgcc ctggaaaaat ctcatgcgaa gaccctggcc gagaaagagc
     6061 ggcagctctc cgagctcagg gaagagctgg atgcggccgc cgatcgtgcc ggcaaactcg
     6121 ccgctgacga gtcgattggg gaagcacaac ccgaacaccc ggcccaggac aagaaagtcg
     6181 aggtgctgca gcagcaggta accacgctgg aggagctcat cagagagcga accgaggagc
     6241 tcaacgagct caagtggcgg gctcagctcg ctgacggcga gcgcgaggcg atcaccgagg
     6301 acgacgccaa gatgatgctg gtgctgaacc agcagctcgg cgaagctcgt gagagcaacg
     6361 accgcctcat agccgtcatc ggtcgtttag aggagcggct cgccgcaagc gatctgggtc
     6421 ccgccggcga cgacttcacg cgaatgcgcg gtatcggcga caagctggcc gcgcagctca
     6481 acgagctggg catacgcacc ttcgggcaga ttgccgacat tgatcttgcc gctctcgaga
     6541 acgaccacca tcccctggcg gcgttcaagc atcgaatcga aaaggacgac tgggtcgctc
     6601 aggcccgcga gcttgccggg agaggctgat ggcctcttga gcggctcgtc gccgtgggtc
     6661 cctcgcgggc aaaggacccg gcttgctcac cttgagtttt ccgcgggtaa ctcgctatag
     6721 tctgctgcgc tcgccgccct ttcgattgca gctaccagga ggtttcatgt cgcactcatc
     6781 cgtgccgttg gttttgcgcc gtacgacgag ttttctgttc atggtcgttc tggtcccgat
     6841 ggggctgggt gtgggctcgg cggtcgccga cgtcgccgcc ggcaaggcag cgtacatggt
     6901 ttgcgcgagc tgtcacgggc agaacggcga gggcaacgcg gccctgaacg cgcctcgcat
     6961 cgccggtcag gagccctggt acatcagccg tcagctggcc gcttaccggg ccgggcatcg
     7021 cgggacggcg cccggagata tctacggcat gcagatgcgg cccatggcca tgacctcggc
     7081 ggacccggca accgaggcca atctcatcga gtacatcgga acgctttccg cggcgcctgc
     7141 cacggcgacc atcgaaggcg acgccgctgc cggccagaat ggctacgcgg tgtgcgtggc
     7201 ctgtcacggt gcgaacggcg agggcaatca ggccctggga gggcctaagc tggccggcca
     7261 gaacgactgg tacctcgtgc gccagatcaa gaattatcag agcggcctgc gcgggtacga
     7321 tcccgccgat acctacggca tgcagatgaa gcccatggct ggcgttctgg ctgacgacaa
     7381 agccatcaac gacgtggttg cgtacatcaa cacgctgcgc tgatcgtgcg cggccggctg
     7441 cacgtcgacc tggctgcgct cgccgcaaat taccggtcgt tctccagcgc ggctgaggcc
     7501 gcaggcggaa ccgcgggtgg ggtcgtcaag gccgacgcct acggtctggg cgccgcgagg
     7561 gtggcggcaa ctctgcagtc tgagggttgc gcggagttct tcgtagccac cgtggaggaa
     7621 ggcgccgcgc tgcgggccat cctgccggaa gccatcattt acgttttcga aggcgccacg
     7681 ggggcaaccg cggaagtgct ggcatccaac gacctcgttc ccgtgctcaa ccatcggggt
     7741 cagctcgaag cctggcaggc ttaccgggag cgtcctgccg ccgtccatgt ggacaccggt
     7801 atgaaccggc tggggttccc cgcggacgtc gatgcggtgc agtttgcggg gatgaactta
     7861 agccttctgc tcacccatct ggcctgcgcg gacgtgcccg atgaccccat gaatcacgag
     7921 caggtcgagc gcttccggcg cgccgccacg tcgtttccag gtgtacgcac gagcatcggc
     7981 aactccgcgg gcatgttggc cgacgcgtcg tgctgcggtg accttggccg tcccggtgtc
     8041 gggctctacg gcggcaatcc cttctccgac cgacgcaacc ccatgcgccg ggtggccgcc
     8101 ctccaagggc aggtcctgca ggtgaggacg tgctcggctg gcagcccgct gggctatggg
     8161 gcgacctacg ttgcgcccgc cgatctgcgg gtggccgtgg tggggctggg ttacgcggac
     8221 ggcctgccga ggctgatatc gggccgaggc tgcgctgccg tgggtggtcg gcgctgcgcg
     8281 ttgctcggcc gggtttccat ggacctgacc ctggtggacg taaccgggct cgaggttcag
     8341 gccggcgact ggatcgagat gtttggcgac acggtgtccg tcgacgaggt tgccggctgg
     8401 gcggagacca tcgcttacga gattctcacc ggcatcggcg atcgcgtgga gcgcgtctac
     8461 tccggggatt gaccctcgag ctccattggc cggtccaggg gctctgcggc gggtcccgct
     8521 tgcaggggta tcggctcgcg gcttcggctc acgggcaggt ccgtctgcgc ggcagatgcc
     8581 aggtcggcgg cggcgaggtc cagctcgccc agggcccgat acacgaagga gcggttgaac
     8641 cgtgccgttg cgcgatgctc ctcatcgttg acgatgacgt tctcgaacgc tttgagcgcg
     8701 tcggggtaga gccccatgtg aaggagcgcg gttccgcggt tgacctctgc cgcgcccaac
     8761 ccaggggcca gagcgagagc cgcttcgaag tcggctagcg ccgcctccaa ccctcccagc
     8821 ctggtcagga tcagacctcg gttgttgtgc gccgctgcct gccggagagt ctcgtccaag
     8881 ctgtcggcgg tcagtctcag aaaaacgtcg cagggctcgg gattggggtc tgctgccgtg
     8941 acttcgcgat ggcagccgag tgccgcttcg tcggccacga gcagctcggc cgccgactgt
     9001 atcgagccgg gcgcgccgcc agcttccccg cctcgctcca cgcccgcgtc cacagcggtc
     9061 tcggccaacg cagcacctgc cgtcagagca atcagcatcg agaaggtgat aatcgcggtt
     9121 cgggcgagca tgaggcgcag tgtaacgcaa tggcggcagg cgcagcgcgg acgcagcgcg
     9181 ggcgctgcga ccgcgctgct ttgccgtagc gctcggaccc gccgccccgt aagcttcgcc
     9241 ttccgcctgt cgggagacgt tcatggccga tccgctgcac aatcagtttc cgcccggctc
     9301 cctggaggcc agggatctgg agcaccttct ccaccccacg acgaatctgg aacagcatca
     9361 caaggtgggt cctcgtgtcc acgaccgggc cgaaggcgtc tatctctggg attcccaggg
     9421 ccgtcagtat ctcgaaggca tggcgggcct ttggtgcacg gcgctcggct acggggagga
     9481 agagctcgtc agcgcggccg cgcagcagat gcgcaagctc agctactcgc agctgttcgg
     9541 cggccgcagc aacgagccca gcatactcct ggccgagcgg ttgaaagcca tggccccctt
     9601 cgatgccggt agagtcttct tcggtctctc gggcagcgat gccaacgaca cccagatcaa
     9661 gctcatgtgg tactaccaca atgccatcgg caagccggaa aagaagaaga tcatcagccg
     9721 catgcgcggc tatcacggcg tgacggtggc cgccgggtcc ctgaccggtc ttcccgcctt
     9781 ccacgcgcac ttcgatctgc cgatcccggg ggtgctgcac gtcgatgctc cacactacta
     9841 tcgcgaagcg aagggcggcg agaccgagac cgagttcgtc gaccggctgg tcggcgatct
     9901 ggaggagctg attcttggcg agggccctga aaccattgcc gcgttcatcg cggaacccgt
     9961 tcaggccgcc ggcggggtga tcgtgccgcc gcccggctac ttcgccaagg tgcaggcgct
    10021 gttggcccgt catgacatcc tcttcataga cgacgaggtc atctgcggct tcggccgcac
    10081 gggccgcgcg ttcggtgcgc agacgttcgg gatcgagccg accaccatga gcgtggcgaa
    10141 gggcgtctct tcggcctacc tgccgctaag cgccgtgatc attcccgagt tcatgtacga
    10201 accgctggtg gagcttagcg ggagcgtggg aaccttcggc cacggcttta cctattcggg
    10261 ccatccggtg tgtgcggcgg ttgcgcttcg gacgctagag ctcatggagg agcgcgagat
    10321 ctttgctcac gcagcagccg tgtcggagcc gttccaggaa cgtctggcgt tgctggccga
    10381 ccacccactg gtgggggacg cgcgcggggt cgggctcctt ggcgcggtgg agctcgtggc
    10441 ggacaaggac acccgcacgc cctttcctcc cgggcagggt gtgggcgcgc attgccagga
    10501 acgttgcgag tcccacgggt tgatcctccg agcgctcgga gacgtggtgg ccatctgccc
    10561 gccgctgatc atatccgagg atcaggtcga cgagctcttc gcgaaactgg aaagggcgct
    10621 ggacgacacg ttcgcctggg ttcactgaac agcgagcgtt cgcgtcacag ctgcggcgcc
    10681 ttggcaggca gttcattggg ttgacgtcgt tgtgtatctt tgccgtctgg ggggagttat
    10741 ggccattctg caatcgaata tcgataccgg cgccgccgcg ttcgccgcca acgcgaagca
    10801 ccaccgcgag cttgccgatg agttgaggac gcttgccggg cgcatcgtcg agggcgggga
    10861 cgcccgctcc agggagcgtc acgttggtcg cggcaagctt ctgccgcggg accgcctcag
    10921 ccgtctgctg gatccgggtt caccatttct ggaggttggc cagcttggcg gctacgagct
    10981 ttacgacgac tggattccca gcggcggcat cgtcaccggc atcggcaggg tctcgggcac
    11041 gcagtgcatg gtggtggtga atgacgccac cgtcaaaggc ggaacgtatt acccgattac
    11101 cgtcaagaag cacttgaggg cgcaggagat cgcgctggag aatggcttgc cgtgcgtcta
    11161 tctggtggat tccggcggcg cgttcctgcc catgcaggcg gaggtctttc ccgaccggga
    11221 tcacttcggt cggattttct acaaccaggc caatctctct gctcgcggca tcccccagat
    11281 cgcggtggtg atgggatcct gcacggcggg gggcgcttac gtgccggcca tgtgcgacca
    11341 ggccatcatc gtccggggcc aggggacgat ctttctcggc gggccgcccc tggtaaaggc
    11401 cgcgaccggc gaggaggtgg atgcggaaag ccttggcggc ggtgacgtgc acagccgcac
    11461 gtcgggcgtg accgactatc tggcggcgga cgatgtccat gccctgggca tagcgcgcga
    11521 aatcgtcgcg gccagccagc agcgctatcg cgcgacggtc gagacccgca cgccccgacc
    11581 tccggccttt gccgaggagg agctctacgg ggtgctgccc acggatccgc gcaccccggt
    11641 ggacgtgcgc gagatcatcg cccggctggt ggacggatcg gaatttcacg agttcaaagc
    11701 gctgtatgga gaaacgctgg tctgcgggtt tgcccacatc gaggggatgc cggttgccgt
    11761 gatcgccaac aacggcatcc tgttctccga gtcggcgctc aagggcgcgc acttcattca
    11821 gctcgccgac cggcgcgggg tgcctctgct ctttctgcag aacatcaccg gcttcatggt
    11881 gggcaagaag tacgagaatc agggcattgc gaaagacggg gcgaagatgg tcacggcggt
    11941 ggcctgcgcg cgggtaccga aattcaccgt cgtgatcggt ggctctttcg gtgccggcaa
    12001 ctacgccatg tgcgggcgcg cctacggcgc gcgcatgctc tggacgtggc cgaacgcccg
    12061 gatatccgtc atggggggag agcaggcggc ccacgtgctg gccaccgtac gccgggacgg
    12121 ggcagccgct cgcggggaag agtggccgga ggctgagcag gcggcgttca tggacgacat
    12181 ccgtgctcag tacgaaactc agggacatcc ctacttcgcg agcgccaggc tctgggacga
    12241 cggcgtcgtc gacccggctg atacccggcg cgtgctgggc ctcgcgctcg aggttgcctg
    12301 ccgggaaccg ctgcagcctc agtccggctt cggcgtcttc aggatgtagc catgttcgat
    12361 actctgctga tagcaaatcg tggcgagatc gcctgcaggg tgatgcgcac cgccacgcgc
    12421 atgggcttac gcacggtcgc cgtgtactcc gatgccgacg cccacgcgca gcacgtgcgt
    12481 ctggcggacg cggccgagcg gatcggcccg gcaccggccc gcgagagcta cctgcgcatc
    12541 gacgcggtgc tcgacgccgc ggagcgccat ggggcgggcg ccgttcaccc cggctacggg
    12601 tttctctccg agaacgcgga gtttgccgct gcctgcgagg agcgcggcat cgttttcgtg
    12661 ggcccttcgg cccgcgccat tcgcgccatg ggctcgaaga tcgaagcgaa acgcatcgtg
    12721 gccgcggcgg gaacgcccgt cgtgccgggc tatcaggaag cggatcagag cgatgcggcg
    12781 ctgtcccgcg cggcggccga gatcggttac ccgctgctca tcaaggcctc ggcagggggc
    12841 ggcggcaagg ggatgcgtct ggtgcgggaa cccgatgagt tcccggctgc cctggccggt
    12901 gcccggcgcg aagcggcggc ggcctttgca gacgagctcg tgctgctcga gcgctacctg
    12961 gatgcgccga agcacctcga ggtgcagatc ctcgcggatc aaaccggcca cacgctccac
    13021 ctctacgagc gcgactgctc ggtgcagagg cgccaccaga aagtgatcga ggaggcgccc
    13081 gggccgacgg tttcggagga gctgcgtacc ctgctcgggg aggcagccgt gcaggcggcc
    13141 aaagccatcg gctacgtcgg agcaggcacc gtggagttca tctcgcaggc gggcgagttc
    13201 tacttcatgg agatgaacac gcggctccag gtcgagcatc cggtcacgga agcgatcacc
    13261 gggctggacc tcgtcgagtg gcagctgcgg gttgccgcgg gcgaaaagct gcctttccga
    13321 cagcatgaga taccgcgtaa cggccacgcg gtggaagcgc gcgtttacgc ggagaatccg
    13381 gcccggcgtt ttcttccttc gacgggaacc cttgcccatg tggcttttcc gcaagaggtg
    13441 cgtgtcgatg cgggggtggc caccggggac gctgtgagcg ttcactacga ccccatgctt
    13501 gcgaaggtga tcgcccacgg gcccgatcgt gacgccgcga tcacctccct cgagcgggcc
    13561 ctggcgcgca ccgagattgc cggcgtggcg cacaacgtcg cctacctgcg gcggctcctg
    13621 gcccatccgg tctttcgggg cggcgactac acgacgggta tcgccgagga gcatgcggac
    13681 gagctcctcg ccgaggaaga tcacctgccc gccatctgcg cggccatcgc ggccgggcgg
    13741 ctcggcgaca gcgtcgggcc ctgggcccga cgcgacgggt tccggttgaa cgggcctgcc
    13801 tcctttatcc agcgtttcag acgcgaacga aaagaggtag aggtcgtgct gtccgagggt
    13861 caggcccaag tcgacggcga agttttcgat gtggccgatg tcagctggaa cgagggcgtg
    13921 ctggcgctgc gggtggatgg caaccgtgtc ggagcccggg tggtgacggc cgggcccagc
    13981 ctttacgtca tcattggcgg gtcgacggcc cggttcgatc cgggcgacgt cgaaggggcg
    14041 gctcgctccc tcggttcggc tgcgggtaac cgggtcgtat cgcccatgcc cgggcaggtc
    14101 atcgcggtct cggtgaagcc cggtgaccgg gtgaaggcgg gcgccgttct cgccgtcgtc
    14161 gaagccatga agatggagca ctccgttgcc gctccgcgtg ccggccgggt ggcatccgtg
    14221 aactgtcgcg ttggtgatcg ggtggacgaa ggcgtcgagc tagtcgtgct cgaggaatga
    14281 attcctggga ggaccgtgtg tcatgagtgc tgcagaattt gatttcgggt tcgacgagac
    14341 cctgaacatg ctgcgtgaca gcgtgcgcca gttcgcggcc gatgagatag cgccccgcgc
    14401 ggcgcagatc gatgccagca acgagtttcc cagggatctc tggcccaagc tgggcgccct
    14461 cggcctgctc ggtgtcaccg tggaggaagc ctacggcggc gccgggctcg gctacctggc
    14521 gcactgcatc gccatggaag agatcagtcg ggcgtcggct tccgtcgggc tgtcctacgg
    14581 cgcccactcg aacctttgcg tcaatcagat tcgtcgctac gccaccgagg agcagaagca
    14641 gcgctacctg ccgccgctga tttccgggga ttacctgggt gctctggcca tgagcgagcc
    14701 gggcgcgggc tccgacgtgg tcagcatgag gcttcgagcc gaggaccggg gcgacagctt
    14761 cgtgctcaat ggcagcaaga tgtggatcac caacggtccg ggggcggacg ttctggtggt
    14821 ttacgccacg gtggacccga gtctgggtcc tcgcgggatc acggcgtttc tcatcgacaa
    14881 gggcacgccg ggtttcagca ccgctcagaa actcgacaag ctgggtatgc ggggatcgga
    14941 tacctgtgag ctcgtcttcg atgattgcga ggtgtccaag acccaggtgc tcggagaggt
    15001 gggccagggg gttcgaattc tgatgagcgg gctggactac gaacgggtgg ttcttgcggc
    15061 cgggccgctg ggcatcatgc aaagctgcct cgataccgtg ctgccctacg tgcacgagcg
    15121 agagcagttc ggacagccgg taggcacctt tcagctcatg cagggaaagc tcgcggacat
    15181 gtacaccacc atgaacgcct gtcgggcata cgtctacgcg gttgcgcaag cgtgcgatgc
    15241 cgggcgaacg acgcgtttcg acgcggccgg ctgcatcctg ttcgccgccg agaaggccac
    15301 ctggatggcc agcgaagcgg tgcaggccct gggcggcaac ggctacatca acgactatcc
    15361 caccgggcgg ctgctcaggg atgcgaaact ctacgagatc ggtgcgggaa ccagcgagat
    15421 tcgccgcatg ctcatcggtc gggagttgtt cgacgccacc ggatgacctt ggctataatt
    15481 tcgcgcccgg cagcttgcgc tgccctctcg gcatgttttt tccgcccatc tcacctggag
    15541 aaagagattt gcgaagcctg gttgtcagat cgaagggtat gaatttctta ctgattggcc
    15601 tggccctgag cggcgtcgcc ttggtcgctc atgcggtccc gcccggcagc gaggacgaga
    15661 tccgtgcccg caccgcgccg atgggggcac tttgccgagc cggcgagaac tgctcgggct
    15721 ttggggcgcc ggcggcaatg tcggccccgg cggtcgctgg ggctcaagcc cctgcagggg
    15781 cgccggccgc tgcggctcag tccggcgagc agatctacaa ccagttctgt gtggcctgtc
    15841 atgccgtggg tgtggccaac gcccccaagc tgggagatgt ggcggcgtgg gcggaccgca
    15901 tcgccaaagg catggacgtg ctcatggcga gcacgctcaa cgggatcaac gccatgcccg
    15961 ccaagggcac ctgcatgacc tgcacggacg ccgatctgca ggcggccgtg gagtacatgg
    16021 tagacggctc ccggtagccg atcactcacc ctcccaggtc accgaccggt agctcagtgc
    16081 ttccgcaaca tgcggtggcc ccacgtctac atgcccctcg atgtccgcca cggttctcgc
    16141 cactttgagc acgcggtgga atcccctggc ggatagctga aagcggtcca ccgcattggc
    16201 cagcagccgt cggctctccg tgttcaatct ggtcagcctg gtggcgtcgg cccccgtgag
    16261 atccacgttg agccgcccct ggcgcgacag ctggacgcgg cgggcgcctg cgatggcggc
    16321 cttcgtctga tcaacgccgc tgctggagtc tcgggctccc gccagcagca gcgcctttgg
    16381 tatcgggggc actgccacgt ggagatcgat gcggtccagc agggggccgg agatgcgggc
    16441 ccgatatctg cgcacctgct ccggcgtgca ccggcaggcg gattcgctgc agacccgacc
    16501 cgccgggcag ggattcatgg cggcgacgag ctgaaagctc gccggaaaac gggcccggta
    16561 gcgggctcgg gcgatggtga tcgagcgggt ctcgagcggc tcgcgcagca cctccagcac
    16621 gctgggtttg aacaggggca actcgtctaa gaacagcacg cctcgatgtg ccagagagat
    16681 ctcgcccggc tgagggtgtc cgcccccgcc gaccagcgca gggccggaag ccgtgtggtg
    16741 cgggtctctg aatgggggta cgaacggata ttccaggggt agtcccgccg ctgagtagac
    16801 cagcgcggct tcgcgggctt cggcctccgt cagcgcgggc agcaggccgg ccagcctgcg
    16861 cgccagcatc gtcttgccgg ttcccggagg ccccgtcatg agcaggtgat gcccgccggc
    16921 agcggcgacg gccagcgctc gctttgcgcc gggctgcccg atcacctctg ccagggagcg
    16981 gctctcccgt tcagcgttcg cggcgggctt ggcggatggt gcggccgcgg gccggtagcc
    17041 tggctcccgc agcagacgca ccacctcgcg taggtgggcg agaggaacca cccggccggg
    17101 gggcgccatg gcaagctctc cggcattggc cgacggcagc accaggcgcc gctcgtggac
    17161 ttccgttgcg gatgcgagtg cggcgcacag gcagccccgg atggggcgca gctcgccgga
    17221 aagccccagc tcgcccagaa actcgaatcc ctccagaacc cggtggggca gctggccggt
    17281 ggccgccagg atgctgaccg cgatggccag atccagcctg gcgccttcct tggccagatc
    17341 ggcgggcgct aaattcacca ccacctttcc gttgggaaac gacagcccgc agttgtcaat
    17401 ggcgctcttg acccggtcgc gggattctct gaccgcggtt tcggggagcc cgaccagggt
    17461 gaatgcgggg aggccgccgg gaagatgtgt ctccaccgta accgcaggcg tgtccatgcc
    17521 ggcctgggcg cgggtacggg tcaggctttt ctcggtggcc gacttgagcg tatggccgtc
    17581 cggatcgttc accccgatcg gatcaggaat ctgcttccag ggcagcgacc cggcgctcta
    17641 agtcacgggt ggtttcggtc agcctgtcca gagcggccag gtgggcgtcg agctcgtgcc
    17701 ggggaacgag atccacctcg gcaaggccgc gctgcagcgc ggagcgggcc tggtccagga
    17761 ccaggcgcag gctgtcgggc ggcacgaggg atgccagctg ctcggtgagg cgctcgatga
    17821 acgttcccac cacgtcctcg ttgccgggtg agtggtttct ggctgagtgc tcggccattg
    17881 cgcgtccgcc cattggtttg gatgacgacg atggtactca cagctgccgt cgccggtcga
    17941 ctggggaatc ctcgcccagg cgtaagccac cgacccgggc cggtgcaggc cggctctgaa
    18001 ctggcgcgct actttggtgc agatgcctta tttcggtgca acagcccggc tcggaagccg
    18061 gttatctcct gccggtccag ccaagcattc cttcaaccag ctgattttta tgaagaaaaa
    18121 tctggtggca cggaaaatgc tcctttgagc gcgtcccgtc agttctgccg ccctgcggtg
    18181 ggcagaaaag gggggagcga tcacccggtc gcgacacaag gtttttagag caagagcaca
    18241 gaggtttact catgaaaaag atcattaccc cggttgtggc gggaatcctc ctcttcgcag
    18301 cgggtgccag cgcgccagcc gccgccgaag gaccggagtt ctccggcaac atcgcatacc
    18361 tgaccgacta ccgcttccgc ggtatctccc agggagatcg gtcccaggcc ctgcagggcg
    18421 gcttcgacat cgagctggac aacggcctct acgcgggaac ctgggcgtcc aacgtcggct
    18481 tcacggaatg ctgcggctcc gcgggactcg agctgaacta ctatgctggt tttgccggtg
    18541 cgttcagcga ggaagtcgat tacgacgtcg ggttcctctg gtacaactat cccgaggacg
    18601 acgcggaccc ggacctcgac tactacgagc tttacggcag cgtcagtgcc ttcggcggca
    18661 cgttcggggc tgccttctct cccgactact tcgccgagac cggcgacttc tggtacctct
    18721 acggcgacta cagcttcggg gtcgccgaga acttgagcat cgatctgcac gtgggcttca
    18781 acctcttcga ggacgaggaa tccttcgcag cgttcatcgg gcctgaagac ggcgatgccg
    18841 gagacaacta catcgactac agcatcggcg tgtccacgtc cgcgctgggt ctcgacttcg
    18901 gcctctcttt catcggcacg gacctcaaga agagcgagtg cttcggcggc accaagctgt
    18961 gcgacgacac cgtcgtgctc tcggtatcta aaagcctgta attgagagag aatccggggt
    19021 gcacagatct tccacaaaga tcttcccaga gcagtccgcg tcagcgggct gcgctcgggg
    19081 agagcgtgca ccttagaaac cgaaaacggg ttcaacggga gaacaaaaca tgaaactggt
    19141 aacagccatc atcaagccct tcaaactcga tgatgtacgg gaggcgctct cggatatcgg
    19201 agtgcagggg atgacggtca ctgaagtgaa agggttcggt cgccagaaag ggcacaccga
    19261 actctatcgg ggggcggagt acgtggtcga cttcctgccc aaggtgaaga tcgagatcgc
    19321 catcgacgac gggctcttag agcaatgcct ggagaccatc accaaatcgg ccaacaccgg
    19381 caaggtgggc gacggcaaga tattcgtttc ctcgctcgaa gacgtcgtgc ggatccgcac
    19441 gggagagacg ggggccgagg cggtctgacg ccatgcgcta gtcgccttta aaaaaattcg
    19501 gagaagattg tggaagatct gttgactcaa actaattacg ccctggacac cttttatttt
    19561 ctggtgatgg gtgcgctcgt catgttcatg gcgcccggtt tcgcgatgct cgaggccgga
    19621 ctggtacggt ccaagaacac ggcggaaatc cttctgaaga acgtcgccct gttcggcatc
    19681 gcgtgcatca tgtacctgat ctgcgggtac tacatcatgt acgtcggcgc cgtcgagggg
    19741 gggatcctgc ccgacttcgg cctgtggatc ggtgcggaga acacggtgga tgaaaccctg
    19801 gcctcgggag gcgacgtcta ctactcggcg cgctccgact acttcttcca ggtggttttc
    19861 gtggctaccg caatgtccat cgtctcgggg gcagtcgccg agcgcatgaa gctatgggcc
    19921 ttcctggcct ttgcggtggt catgacgggc ctgatttacc cgctgcaggg catgtggacc
    19981 tggggcggcg gctggatctc cacggccggc tactcggact tcgccgggtc cggcatcgtg
    20041 cacatgtgcg gcgcggcggc agcgctggcc ggggtcttgc tgctcgggcc aagaaagggc
    20101 aagtacaaca aggacgggtc ggtgaacgcg ctgcccggcg ccaacctgcc cctggcgacc
    20161 ctcgggatgt tcatcctctg gttcggctgg ttcggattca acggcggctc ggagctcaag
    20221 acgtcgaaca tcgacgaggc gaacgcggtg gcccaggtgt tcgtcaacac caacatagct
    20281 gcctgcggcg gcttggttgc cgcgctgctg accgccaaga tgctcttcgg gaaggccgat
    20341 ctgaccatgg ctctgaacgg ggcgctggcc ggtctcgtgg ccatcacggc ggatccgctc
    20401 tcgcctatcg ctcagtgggc gatgatcatc ggcgccgtcg gcggggttct agtggtgttt
    20461 tccatcgtga cgctcgacaa gctgaagata gacgacccgg taggtgccat ctcggtccac
    20521 ggcgtggtcg gtatctgggg catcctcgcc gtggtcttca acaacggcga cgccaccatt
    20581 ggtggccagc tgatcggcat cgccgggata ttcgcctgga ccttcgtcgc agcgctcgtc
    20641 gtctggggga tcctgaaggc caccatgggc attcgggtct cggaagagga agagtacgaa
    20701 ggcgtcgacc tttcggagtg cggtctggag gcctatccgg agttcgccaa ggtctcctcc
    20761 tgagcttcgc cccagcgggg cggacggcaa cgaacgccgg ccacattcgt ggccggcgtt
    20821 ttcgtctgta acgggtacaa tgcccaattt ttcaggcacc cgtccc
//
LOCUS       DBNHLM010000169         3804 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_562919, whole genome shotgun sequence.
ACCESSION   DBNHLM010000169 DBNHLM010000000
VERSION     DBNHLM010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_562919"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="AC16QF_07750"
     CDS             <1..576
                     /locus_tag="AC16QF_07750"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448489.1"
                     /translation="VAATVENFGGLDILVNNAGNWRRTPVDASWEQALADWDFIMDTN
                     LKGLLMLSRACVPHLQSRGGGDIVNISTYYVLPAKSEGTNSPETDLYNASKWAINGFT
                     DAWAKYLAKDNIRVNGMCMGATDTPMLRGLFDDGQLPPDLAETVMRPAQIAQQMIDLL
                     ADGRSGENIGAWVGEPIVIPPHPPEHRRISG"
     gene            complement(585..1484)
                     /locus_tag="AC16QF_07755"
     CDS             complement(585..1484)
                     /locus_tag="AC16QF_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6448490.1"
                     /translation="MEFFANYPASTGVKPDVWAREKEAQGWHGICASDHLWVGATRYP
                     HVFVAATQMACATTRTLITTSFCNNLFRSPVEFAQAALSLQAASGGRFEAGLGAGWLQ
                     DEIEAMGDVYPSGPERVSRYVEALTVVRSLLATGQCDFQGDHYQVRIQGEHALGLVTD
                     SPPPLIASAGGPRALREVAPLVDRVEVKASARATRVGYLDFEIMATVTEEEVRGQVER
                     VRAVNADVPLGIFILTGTGDNPAVKGLKAALANGYLSRFVGEPEQVAEALNQLGEIGI
                     DRVQLTELVPGSHAELAPYLLSG"
     gene            1634..2425
                     /locus_tag="AC16QF_07760"
     CDS             1634..2425
                     /locus_tag="AC16QF_07760"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448491.1"
                     /translation="MQAADWIDLSGKVAHVTGAGKGIGAGIARALAMAGATLAVSDVD
                     GEAAARTAEEIGGLGMQLDVSDRAAVDAALERTADELGALDILVNNAGVYIGFGGPVR
                     EITDEMWRTLWSINVDGVFYCCRAAARIMIEAGNGGRIINIASTQAVSPGVGVTYDGS
                     KAAVTQMTKALALELAPHRINVNALAPGPTWTREGDPPPTGGQPPAPTGDPLADTVAD
                     RIARLPAGHWGDPMEQGKAAVFLASSLSDFVTGIYLPVDGGWLTL"
     gene            2448..>3804
                     /locus_tag="AC16QF_07765"
     CDS             2448..>3804
                     /locus_tag="AC16QF_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR6448492.1"
                     /translation="MTAGLRSKHNEAAALVAEMTLEEKAGLCSGRDFWHLKPVARLGL
                     PSVMVTDGPHGLRKQDAEADHAGLNASVPATCFPTASALACSWDRELLQEIGVALGEE
                     CVAERVAVLLGPGMNIKRHPLCGRNFEYFSEDPLLCGELAAALIDGVQSQGVGTSVKH
                     FAANNQEHGRMTVNAIVDERTLREIYLRGFEIAVRNSQPWTVMCAYNRLNGTYCSEHA
                     WLLDSVLRDEWGFAGLVVSDWGAANERARGVAAGLDLEMPASGGINDARVAAAVRSGE
                     LAEAVLDRAATRNASLALLGGELEGRPRDVDHDAHHGLARRAAAQSAVLLKNDGGLLP
                     LGRDVSIAVIGAFAQRPRYQGAGSSQVRPTRLDCAWDAMEKIAEAGRLTFAAGYDPRR
                     SEPDQALIDEAVDLARAAEVAVVFAGLPGIYESEGFDRAHMRLPDQHNRLIAAVCDAN
                     PS"
BASE COUNT          609 a         1270 c         1334 g          591 t
ORIGIN      
        1 gtagccgcca ccgtggagaa cttcgggggc ctggacatcc tggtgaacaa cgccggcaac
       61 tggcgccgca cgccggtgga tgcgtcctgg gagcaggcgc ttgcggactg ggacttcatc
      121 atggacacca acctgaaggg gcttttgatg ctgtcccgcg cctgcgttcc gcaccttcag
      181 tctcgcggcg gcggcgacat cgtcaacatc agcacctact acgtgctccc ggccaagtcg
      241 gagggcacga actcgccgga gacggatctc tacaacgcct cgaagtgggc catcaacggc
      301 ttcacggatg cctgggcgaa gtacctggcc aaggacaaca tccgggtgaa cggcatgtgc
      361 atgggcgcca cggacacgcc gatgctgcgc gggctttttg acgacgggca gctgccgccg
      421 gatcttgccg agacggtgat gcggccggcc cagatcgccc agcagatgat cgatctgctc
      481 gccgacggcc gctcaggtga gaacatcggt gcctgggtgg gcgagcccat cgtcatcccg
      541 ccgcacccgc ccgagcatcg gcgcatttcc ggctgagccg gcgctcagcc ggagagcagg
      601 tagggcgcga gctctgcgtg ggagccgggc accagctcgg tgagctggac ccggtcaatg
      661 ccgatctcgc cgagctggtt cagagcctcg gccacctgct caggctcgcc tacgaaacgg
      721 gaaaggtacc cgttcgccag cgctgccttc agccccttca cggcgggatt gtcccccgtg
      781 ccggtgagga tgaagatgcc gagcggcacg tcggcgttca ccgcgcggac gcgctcgacc
      841 tggcctcgca cctcttcctc ggtgaccgtc gccatgatct cgaagtccag gtagcccacc
      901 cgggttgccc gggcgctggc cttcacctcc acccggtcca ccagcggcgc cacctcgcgc
      961 agcgcgcggg ggccgccggc ggaggcgatc agaggcggcg gagagtccgt gacgagcccc
     1021 agggcgtgct caccctgaat ccgcacctgg tagtggtcgc cctggaagtc gcactggccg
     1081 gtggcgagca gagaacgaac gacggtcagt gcttccacgt atctggatac ccgctcggga
     1141 cccgacgggt agacgtcgcc catggcctcg atctcgtcct gaagccagcc cgctccgaga
     1201 cctgcctcga accgcccgcc ggatgcggcc tgcagtgaga gggccgcctg ggcgaactcc
     1261 accggcgagc ggaacaggtt gttgcagaac gacgtggtga tcagcgtgcg cgtggtggcg
     1321 caggccatct gagtggcggc gacgaacacg tgggggtagc gcgtcgcgcc cacccagagg
     1381 tgatcgctcg cgcagatacc gtgccagccc tgtgcttcct tctcccgggc ccacacatcg
     1441 ggcttcaccc cggtgctcgc ggggtagttg gcgaaaaatt ccataggacg tctacctccc
     1501 tgcacccgtt ctgctcgtcc cgggcgctgc ccgcgacaat actaacccaa aggatcggga
     1561 cactcgtcgt cgcggccgga gcagccggga tgatgtgcta cagtctcgct aagaatgacg
     1621 acgcggggtg ggcatgcagg ctgcggactg gatcgatctg agcgggaagg tcgcccatgt
     1681 gaccggcgcc ggtaaaggca tcggtgcggg catcgcgcgg gcgctggcca tggcgggggc
     1741 cacgctggcc gtgagcgacg tggatggcga agcggcagct cggaccgcgg aggagatcgg
     1801 cgggctcggc atgcagctcg acgtgagcga tcgtgcagcg gtggacgccg ctctcgagcg
     1861 cacggctgac gagctcggcg cgctggacat cctggtcaac aacgccggcg tctacatcgg
     1921 gttcggggga cccgttcgtg agatcaccga cgagatgtgg cgcacgctgt ggtccatcaa
     1981 cgtggacggg gtgttctact gctgccgagc ggccgcgcgg atcatgatcg aggccggcaa
     2041 cggcggccgc atcatcaaca ttgcctccac ccaggcagtc tcgccgggcg tgggcgtgac
     2101 ctacgacggt tccaaggcgg cggtcaccca gatgaccaag gccctcgccc tggagctggc
     2161 accccaccgc atcaacgtca acgccctggc accaggcccc acctggacgc gcgaagggga
     2221 cccgccgccc acgggcggcc agcctcccgc accgactggt gacccgctgg cggacacggt
     2281 ggccgaccgc atcgcccggc tgcctgccgg ccattggggc gaccccatgg agcagggcaa
     2341 ggcggcggtc tttcttgcgt cttccctgag tgatttcgtc accggtatct atctgccggt
     2401 ggacggtggc tggctcaccc tctagcgcgc tcgatcggag gagcgtcgtg acggcagggc
     2461 tcaggtccaa gcacaacgag gcggccgctc tcgtcgccga gatgaccctg gaggagaagg
     2521 ccgggctctg ctcgggccgg gatttctggc atctgaaacc cgtggcgagg ctgggattgc
     2581 catcggtgat ggtgaccgac ggcccccatg gtctgcgcaa gcaggatgcg gaggccgatc
     2641 acgcggggct gaacgccagc gtgccggcca cctgttttcc caccgcgtcg gcactggcgt
     2701 gctcgtggga ccgcgagctg ctgcaggaga tcggcgtggc cctcggagag gagtgcgtgg
     2761 cggaacgggt ggccgtgctg ctggggcccg gcatgaacat caagcgtcac cccctgtgcg
     2821 ggcggaattt cgagtatttc tccgaggatc cgctgctgtg cggcgagctg gccgcggccc
     2881 tcatcgacgg tgtccagtca cagggggtcg gcaccagcgt caagcacttc gcggccaaca
     2941 atcaggaaca cgggcgcatg acggtgaatg ccatcgtcga tgagcgcacg ttgcgggaga
     3001 tctacctgcg gggattcgag atcgcggtgc gcaactccca gccgtggacg gtcatgtgcg
     3061 cctacaaccg cctcaacggg acctactgca gcgagcacgc ctggctgctc gattcggtgc
     3121 tgcgcgacga gtggggcttc gcgggcctcg tggtcagcga ctggggtgct gccaacgagc
     3181 gggcccgggg ggtggctgcc gggctggacc tggagatgcc ggccagcggc gggatcaacg
     3241 atgcccgggt ggcagccgcc gtgcgctcgg gcgagttggc cgaggccgtg ctcgaccggg
     3301 cggcaacccg caatgcgagc ctggccctgc tcggcggcga gctggaaggt cggccccggg
     3361 acgtcgacca cgatgcccac catggcctgg cgcgccgggc ggcggcgcag agcgccgtgc
     3421 tgctgaagaa cgacggcggg ctgctgcccc ttggcaggga tgtatccatc gccgtcattg
     3481 gtgcctttgc ccagcgtccc cgatatcagg gcgccggaag ctcccaggtg cgcccgaccc
     3541 gcctggattg cgcctgggac gccatggaga aaattgccga ggcgggccgg cttaccttcg
     3601 cggccggcta cgatccgcgc cgcagcgagc ccgatcaggc tctcatcgac gaggccgtcg
     3661 acctggcccg cgccgccgag gtggcggtgg tgtttgccgg cctgccgggc atctacgaat
     3721 ccgaaggttt cgatcgtgcc cacatgcgcc tgcccgacca gcacaaccgt ctgatcgcag
     3781 ccgtctgcga tgccaacccc agta
//
LOCUS       DBNHLM010000170         2761 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_922243, whole genome shotgun sequence.
ACCESSION   DBNHLM010000170 DBNHLM010000000
VERSION     DBNHLM010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_922243"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..822)
                     /locus_tag="AC16QF_07770"
     CDS             complement(55..822)
                     /locus_tag="AC16QF_07770"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448493.1"
                     /translation="MSSMRGKVALVTGASGGIGLATAIGFAERGAAVMLAARRSDELE
                     RAVREITASGGEAAAIQTDVASSEQVQEMVDRTVGTFGRLDYAVNNAGIEGVPAEFVD
                     FDEDEWDRVMNINLRGNFLCLKYQAKAMLACGHGGAIVNVGSVNSFLGFAGGAAYATS
                     KHGQVALTSSASAELAPKGIRVNIVCPGVIDTPMHRRLRGTFGDEAYDEILSRVHSRR
                     AGRPEEIASTIVFLCSDEASYLTGATITPDGGYLTTM"
     gene            complement(852..2456)
                     /locus_tag="AC16QF_07775"
     CDS             complement(852..2456)
                     /locus_tag="AC16QF_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR6448494.1"
                     /translation="MSAEFQFGDWTIETSLNRITRADCEQHLEPKTMAVLVFLLNRHG
                     EVVSVDEMLDRLWTGRIVGPNALHRHIALLRRALGDDPSTPSYIETITKRGYRTIASM
                     IRLPPHGAAPSDDSQHDRQEADSWGTRIAVSPFLDRTTGKSHHHLVDGMAEEVVAGLA
                     SFSDIGVIPPSALPRGDASGWDPLDTCAAVGADFFLEGSVSDSASRVRVSLRLTDVAN
                     REVIWSRSFDYVLSASSYFDIQDDVKARVSAGIADSFGVVSSKAYERARTKPTSAIAA
                     DDAVMLAKGHEGNPKLHREAREGLEKAVEQHPDFANAWAWLGAMYRDEFVFWANERPN
                     PLERAESAAQRALKLDASNQEALLVLADVRFYQGDLAAFRQASERAMSLNPSSPDLLS
                     VVASHLIHAGYYEEGKPLAQRAVDLLAQPTLHMLGTLSVCHYGLGDYAAAARVIRQVW
                     AGVDRNRISGMQAYYLTLLVAATAKLGREDEAGQYLQWILKSIPNYAQIMGPSWHRQG
                     ACEDLRERLAEGLAAAGLRIGELTNR"
BASE COUNT          470 a          875 c          870 g          546 t
ORIGIN      
        1 aaagaataaa cattttgccg cgcacatgcc gtaattgctc gggctgccgg ttgcctacat
       61 cgtcgtgaga tagccaccgt cgggggtgat ggtggccccg gtcaggtagc tggcctcgtc
      121 cgagcacagg aacacgatgg tgctggcgat ctcttcgggg cggccggccc gccggctgtg
      181 cacgcggctg aggatctcgt cgtaggcctc gtcgccgaag gtcccgcgca ggcggcggtg
      241 catgggggtg tcgatgacgc cggggcagac gatgttcacc ctgatgccct ttggcgcgag
      301 ttctgccgat gcagaagagg tgagcgctac ctggccgtgc ttggaggttg cgtaggcggc
      361 gccgcccgcg aagcccagga acgagttcac cgagccgacg ttgacgatgg ccccgccgtg
      421 gccacaggcc agcatcgctt tcgcctggta cttcagacag aggaagttgc cgcgcagatt
      481 gatgttcatc acgcgatccc actcgtcctc gtcgaaatcc acgaactctg ccgggacccc
      541 ctcgatgccg gcgttgttga ccgcgtagtc caggcgcccg aaggttccca cggtgcgatc
      601 caccatctct tgcacctgct ccgacgaggc gacatccgtc tggatcgccg ccgcctctcc
      661 gccggaggcc gtgatctccc gcaccgcccg ttccagctca tcggaccgtc tggccgccag
      721 catcaccgct gcaccgcgct ccgcgaagcc gatcgccgta gccaacccga tgccgccgct
      781 tgcccccgtc acgagcgcca ccttgcctct catcgaactc atctcccatt gtccttatgc
      841 aaccgtctag cctaacggtt ggtgagctca ccgatccgga ggccggctgc ggcgagccct
      901 tccgccaacc gctcgcgcag atcctcgcac gccccttgcc gatgccagct ggggcccatg
      961 atctgagcat agttggggat gctctttaaa atccattgca ggtactggcc ggcttcgtcc
     1021 tcgcgcccga gctttgcggt ggcggcgacc agcagggtca ggtagtaggc ctgcatgccg
     1081 ctgatccggt tccggtccac ccctgcccac acctggcgaa tcacccttgc ggcggcggcg
     1141 taatcaccca agccgtagtg gcacacagac agcgtgccga gcatgtgcag cgttggctgt
     1201 gccagcagat ccacggcgcg ctgggcgagc ggtttgcctt cctcatagta gccggcgtga
     1261 atcaggtggc tcgccaccac ggaaagaaga tcggggctgc tcggattcag gctcatggcg
     1321 cgctcggatg cctgtcggaa cgcagccagg tctccctggt agaaccgcac gtccgcgaga
     1381 accagaaggg cttcctggtt ggaggcgtcg agtttcagcg cgcgctgtgc tgcgctctcc
     1441 gcacgctcga gtgggtttgg ccgctcgttt gcccagaaca cgaactcgtc ccggtacatg
     1501 gcgccgagcc atgcccaggc attggcgaaa tcaggatgct gctcgaccgc tttttcaagg
     1561 ccctctcgtg cctcacgatg cagtttcggg ttgccctcgt ggcccttggc gagcatgacg
     1621 gcgtcgtccg ctgcaatggc cgaagtcggc ttcgtccttg cccgctcata ggccttcgaa
     1681 gagaccacgc cgaaagagtc tgcgatgccg gcagagaccc gggcttttac gtcgtcctgg
     1741 atgtcgaaat agctgctcgc cgagagcacg tagtcgaacg atcgggacca gatcacttct
     1801 cggtttgcca catcggtgag tcgcaggctg acacgaactc tcgaggccga atcgcttacg
     1861 ctgccttcca ggaaaaagtc cgcgccgacg gccgcgcagg tgtccagggg atcccagccg
     1921 gatgcatccc ccctcggcag ggcgctgggc gggatgacgc cgatatcgct gaagctggcc
     1981 aaccccgcga cgacttcctc tgccatccca tcgaccagat ggtggtgact ctttcctgtc
     2041 gttctgtcga ggaacggcga aacggcgatg cgggtgcccc aggagtcggc ttcctgcctg
     2101 tcgtgctgcg agtcatctga tggcgctgcc ccgtgagggg gaagcctgat catggacgca
     2161 atggtgcgat acccgcgctt ggtaatggtc tcgatgtacg acggtgtgct gggatcatct
     2221 cccagggcgc ggcgcagcag cgcgatatgg cggtggagcg cattgggccc gacgatccgg
     2281 ccagtccaga gtctgtctag catctcgtcg acggacacca cctcgccgtg gcggttcagc
     2341 agaaagacga gcactgccat ggtctttggt tccaggtgct gctcgcaatc tgcgcgtgtg
     2401 atccggttga ggctggtctc aatggtccag tcgccaaatt gaaattccgc cgacacccgt
     2461 ctcccccgtc tcaggtagct tgtcgatccg ctatggcagc gctgaatgct gagcttagcg
     2521 aagggcgcga aatcagtgaa ccatcagaag aacatcaggc tgttggccga cggctaacgt
     2581 aaattgcctg gagggtgccc cctgcaggtg ttgtcacggg agactgtagc cggggctgga
     2641 tggtcgaggt gcacaggagg ttcagttgac tatgaggaac aaaattctct acacgttggg
     2701 atttgctttg ttcgtgttgg tctggctggg agtgatggat gctcgggctg actcccatgc
     2761 g
//
LOCUS       DBNHLM010000171        16454 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_503407, whole genome shotgun sequence.
ACCESSION   DBNHLM010000171 DBNHLM010000000
VERSION     DBNHLM010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 16454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16454
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_503407"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="AC16QF_07780"
     CDS             <1..671
                     /locus_tag="AC16QF_07780"
                     /inference="COORDINATES: protein motif:HMM:NF015406.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HIR6448495.1"
                     /translation="DPREVVDAAVRAHAEGRAPINAVEGFVRQVVGWREFVRGVFHHY
                     YEPMQSRNIWNADRRLTDAWYRGTTGIAPLDHVIGKALRLGWAHHIERLMVAANLMNL
                     SGIEPQEVYRWFMEMFVDAYDWVMVPNVFGMGLTSEGGIFTTKPYICGSNYVMKMGDF
                     KRGEWCDVMDGLLWRFVAHHEETLKANHRLAPMVANLPRVVRKRPEIFALADEFIETT
                     TRAA"
     gene            708..1649
                     /locus_tag="AC16QF_07785"
     CDS             708..1649
                     /locus_tag="AC16QF_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6448496.1"
                     /translation="MSDPEAAVMSIGAVTKATGISTHTLRKWESRYGVIEPLRTPTGR
                     RVFTQSQVDRLILLRDLLAQGHQIGRLSTLADDELEALASATPARSSVPEFGLVTVVG
                     PLISAVLSREALGPAPFRLVTRPATEWLAGGEEGSDQEAQALVVELSTLSEESCGRLL
                     ELRRGVYQRVIVVYGFAARTTLQMLLDGGIVCLKAPVDGQEVLRALAQSEAPGVVAAL
                     ADPTIPAPRFSPDSIARLAAMAPAIRCECPNHIAQLLTDISAFEEYSKLCEQGDPADR
                     VLHARLRLIAANARALFEEAMENVARAEGLELEELDG"
     gene            complement(1669..2775)
                     /gene="modC"
                     /locus_tag="AC16QF_07790"
     CDS             complement(1669..2775)
                     /gene="modC"
                     /locus_tag="AC16QF_07790"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HIR6448497.1"
                     /translation="MSVDELDIEITASLPGFDLRLKRTLPLQGVTAVFGPSGSGKSTL
                     LRVLAGFIRPASGRVSCGGDVWFDKAANVHLPPHRRPVGMMFQDARLFPHLNVAGNLD
                     FAVRRQRRPGNGIEQPEVVAALDLGPLLTRQVDSLSGGERQRVALGRTLLASPRLLLL
                     DEPLAALDRERKADILPYLDQIQRGFGIPTLYVSHDVDEVAQLCDRVLVLAGGSERAF
                     GATADIVERLDLEPLMGRFEAGVLLEGTVTGHDPRLHVTHVDVGGDVLTLPLLSGVAE
                     GHAVRVRIRARDVALATTQPEGLSIRNVLPGRISRLLIEEASGTAEAFVELRGAHLRS
                     RLTLAAVEDLGLREGMPVYALIKSISFESSAPGG"
     gene            complement(2765..3436)
                     /gene="modB"
                     /locus_tag="AC16QF_07795"
     CDS             complement(2765..3436)
                     /gene="modB"
                     /locus_tag="AC16QF_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR6448498.1"
                     /translation="MPDLAPLWLTLHLATATTLILLVIGTPIAWWLARTRSPLRAPVE
                     ALTALPLVLPPTVLGFYLLVMLSPTSPFGSFWVQVTGEALTFSFAGLVVASVIYSLPF
                     MVQPLTNAFSAIGPGPMEAAASLGAGPFDAFRCVAAPLAKRGFVTAAALSFAHTVGEF
                     GVVLMVGGNIPGETKVISIAIYEHVETLRYAEAHLLSAGLVAFSFVVLLTVYGLNRRS
                     PLNVG"
     gene            complement(3437..4201)
                     /gene="modA"
                     /locus_tag="AC16QF_07800"
     CDS             complement(3437..4201)
                     /gene="modA"
                     /locus_tag="AC16QF_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6448499.1"
                     /translation="MSSARKRSRLLQLLATCWLLAGAGNAWAERALAAVASNFVVPAE
                     LLAAEFERATGHTVSIAAGATGKLYAQVVQGAPFDLLLAADAWRPERLELEGRAVPGS
                     RFTYAVGLLTLWSPRPDAFGEDPVGYLRAGRLRRLALANPDLAPYGTAARQVLEHLGL
                     QAQLADKMVFAENIGQAHAMVDTGNADAGFLARAQVIGRPGSHWNVPVGMHLPIEQQA
                     VLLRRAADNAAATGFLGFLRTEAARGAMATAGYGLP"
     gene            complement(4301..5539)
                     /locus_tag="AC16QF_07805"
     CDS             complement(4301..5539)
                     /locus_tag="AC16QF_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6448500.1"
                     /translation="MLVMLALLPASRLAAQDVPSPAFAQQLARVMDRAEEDPEWALER
                     LAEMMRANRNRPTELRYAVRQRAAMLMQTERLDLAAEEMLAAVTGKDPGYAPELRFLL
                     GQVWLLKDDARTALGHLELWARHADPLDPAGLFLVGYAHLQLDEFEPAAKALETAIAT
                     SIKPRPQWFEILAYVYTRLDRPADAIGLLETLIASQPQQSRWWRQLGSIYLLIDDVPR
                     GTAGLTVLAELEPLGLSDSRSLARLFASLDMPADAAYVLDAAMTRARTAAELPGAPSE
                     PDGSEKPEVPQELPGYEDVMLLAELWIMAREFDAAVGTLKTASAMRDDGEAQTLLGQL
                     YLQREDYPQADRHLDAALAAYGEEPPPRLIYLKAVVALNRGDDEAASRALKRISGDEA
                     FAQRVARLQRYIDSRRQRGA"
     gene            complement(5557..6201)
                     /locus_tag="AC16QF_07810"
     CDS             complement(5557..6201)
                     /locus_tag="AC16QF_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6448501.1"
                     /translation="MALRQGFIFLGALAVTLSLYVMMDRMVGRDRTRLLSPVDAQSIE
                     FVRTEMEDRSRTKDRRRKPPPKPQEMKRVRADLQTNLNRAADLPTETLAFNIKSLLGE
                     GGGVALGERLVQGSGAALELVMASDLTPLSMLPPQYPPSALMRGVEGWVDMVFIVTEE
                     GGVVDPTILESRPGTTFDAAAKAAAIRWRFRPVVRNGQPTPIRARIRIDFELPE"
     gene            complement(6201..6611)
                     /locus_tag="AC16QF_07815"
     CDS             complement(6201..6611)
                     /locus_tag="AC16QF_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR6448502.1"
                     /translation="MKLHTREEGGSAAINLTPLIDMVFILLIFFAVNATFVKITGVSV
                     DQPTAKTTSLQPASTILIAVTDAGEIWIDKKKVDKRRVRGVVERMFIEAPDAAVVVLS
                     DEESRTGLVVEVIDQVRLAGAGRVALAAAEPEEQ"
     gene            complement(6608..7147)
                     /locus_tag="AC16QF_07820"
     CDS             complement(6608..7147)
                     /locus_tag="AC16QF_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6448503.1"
                     /translation="MLGDLFGNSDLIMAGGWIIVLIMALTFVMWMMILDRYVYLWRDR
                     GRAVSRKFALWQQYSSPDPLNNKRLRAALTGQFLEDSRRWIGTIQVITAVLPLLGLLG
                     TVTGMIKTFDVLTAFGTGNIRGMAEGISQALITTMAGLIAALTGIYFANDLEERIATE
                     ADHLAGRFNEGFADRGDGR"
     gene            complement(7137..8489)
                     /locus_tag="AC16QF_07825"
     CDS             complement(7137..8489)
                     /locus_tag="AC16QF_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6448504.1"
                     /translation="MRRTAITLLALLAPASWAAAPTTLDELIDSLKDSSALQAKRLQE
                     REARFVAARNERERLLEDARDRRREAEREADRLRADFEGSEQELADLETRLDENAGDL
                     RELFTVVRQITGDVAPMVESSLVSAEQGPRNALLRRLAEPGAIPAAEELRELWMILLE
                     EMNHSGRVSRFEAPVISADGAERTRRVTRIGTFTALADGSYLRYLPETGRLLALGRQP
                     AGVDVGDVLAFEEAREPLQRVAIDPSRGSILSLIVQTPDVRERIEQGGVIGYIILALG
                     ALGLLLGVERLVTIGVARQRTRRVLADSAAAGDHVIVELRELARDEAYLADAEALSAK
                     MDEVVTIASEKLRRGLPTLAIFAAVSPLLGLLGTVTGMIETFQVITLFGAGDPRLMSG
                     GISQALITTQLGLAVAIPLLLIQSFVQGRANALIATLDETAAELFAQGRTHRGSDHAG
                     "
     gene            complement(8486..9274)
                     /locus_tag="AC16QF_07830"
     CDS             complement(8486..9274)
                     /locus_tag="AC16QF_07830"
                     /inference="COORDINATES: protein motif:HMM:NF023358.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 domain-containing protein"
                     /protein_id="HIR6448505.1"
                     /translation="MKPYGKRHASWVWLTLFLLTPNPSRSEAPTATAGDALDQAQSYQ
                     TIAGRSQARVEDLDDAALAMLSQYNGEVDRYEDLRTYNDNLRELLASQRAEKERLNAE
                     LAGIEIVRQEIVPLMVQMIDVLDSFVALDQPLLIDERTSRVESLRRNLTRSDVDLAER
                     YRRIIEAYQIEAEYGQTIEAYEGPIVIDDRELTVDFLRVGRIGLYYLSLDRAEGGIWN
                     PAEKRWTPLAENYLDDLDLAVRMARKQAPPNLMDLPMWTTGGES"
     gene            9412..11088
                     /locus_tag="AC16QF_07835"
     CDS             9412..11088
                     /locus_tag="AC16QF_07835"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6448506.1"
                     /translation="MLSRSEALEALTAPGQPYELVDVELYGRSCRAFRNAPPLLRDLY
                     ASTASDLPFIVYGGERLSFADAWAESCRIAILLMEQYGVARGDRVAISMRNYPEWMTA
                     FAAVTSIGAIAVAMNALWTPEEMEYGLKDSGAKVLLADEERLARLALCADPPAGLAVL
                     AVRAADDAGTGARDLREALAGVPAGNMPAVDVAPEDDATILYTSGSTGHPKGAVSCHR
                     SLVTALLSWELDAHAGAYMQGVDLPAPESQPATLLAVPLFHATGSHAVYLASYRSQRR
                     VVSMYKWDVEEAAELIERERISSFVAPPAMTGDLVQFAARTNRDLSCLDRVGGGGAAR
                     APEQVRGIRRTFAKAMPSTGWGMTETNAIGTVISGQEYLDRPESSGRCSAVLELRVVD
                     PEGRELPCGERGELQVRGTSLIRGYWNRPEATAEAIVEGWLRTGDVAYLDDDGYLFIV
                     DRIKDLVIRGGENIGCGAVEAALQEHPDVIEACVYAVPDERLGEEVGATVYICRPVDE
                     EEIRNFLAPRLARFEIPRYLSFSTQPLPRTASGKIFKRQLKQEAADRLTG"
     gene            11123..12160
                     /locus_tag="AC16QF_07840"
     CDS             11123..12160
                     /locus_tag="AC16QF_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6448507.1"
                     /translation="MKLGILGGNIGRKTGINIEAIRKAESLGYDSVWTAEAWGGDAVT
                     PATWILAQTEKIKVGTAIMQMPARTPAMAAMTAMSLAELSGGRFIIGLGASGPQVVEG
                     WHGVPYGKPMTRLREYVAIMRKIFAREDKLTFDGEMYQIPYTGPGSTGLGKPLKSILH
                     CEEDIRIFAASITPKGVETAAEVADGFFPIWMDPEKFSVFEEPIRKGFAAAGDKDLSS
                     FEVSPFVTVVIGDDVEQCMAPIRGNMALYIGGMGARNKNFYNDYAKALGFEDAAVEIQ
                     DHFLAGRREEAMRAVPAELIDACHLVGPADRIRERLQRWKAAGGNGQVASMLLGTQQP
                     EALELVASEML"
     gene            12203..12766
                     /locus_tag="AC16QF_07845"
     CDS             12203..12766
                     /locus_tag="AC16QF_07845"
                     /inference="COORDINATES: protein motif:HMM:NF025050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6448508.1"
                     /translation="MSNGTMPIFQHAYFVNDIEEAAHRWNRLYGAGPFVLVPHHKTDK
                     FQYRGTDQEADVSYGFGYLGENQIQFIQQHDETPSIYRDMYQAGEEGFHHVGCLVNDF
                     AAARQRMLDLGFVNACELWADDVNAAYFDTRSVNGGFTEIHGDPVHILGTFATWKRAH
                     QNLKPGDSPIMDMSAPFPDGRDPGYLD"
     gene            12773..13951
                     /locus_tag="AC16QF_07850"
     CDS             12773..13951
                     /locus_tag="AC16QF_07850"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase family protein"
                     /protein_id="HIR6448509.1"
                     /translation="MKPQPFRIEVPEETLRDLADRLDRSGWPDDFANDDWSYGTNGAY
                     LRDLVGFWRRDYDWRAVERKINAFDHYRVEIEGMPVHFLREPGRGPNPVPLILSHGWP
                     WTFWDLNEVIRPLADPASFGGDPEDAFEVIVPSLPGFGFSTPLTTPGINFWRTADLWH
                     TLMTEVLGFQRFGAQGGDWGALVTAQLGHKYAESLFGIHLTNLIPLTLFNHQRPWDIT
                     AGVMVPDDVPADERDRQLAQQRRIASHVAVQVLDPQTLAYGLHDSPVGLLAWLLERRY
                     FWGDCNGDVESRFSREHLITTAMIYWVTSSFATSARYYAEAARNPWRPSHDRSPQVEA
                     PAGATYLGGDVGRGTIPAVREAFDLRYENSHPVGGHFAPMEEPEVVINDIRETFRPLR
                     "
     gene            complement(13979..14500)
                     /locus_tag="AC16QF_07855"
     CDS             complement(13979..14500)
                     /locus_tag="AC16QF_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6448510.1"
                     /translation="MTLPISATFHHVGITVSNLERAVEWYSKVFGLKPGVALEVSGPT
                     GEQVLRLPPHIHRAALLPVGDFAIELLEFEPTRRQMDMRQDDVGYVYICFAVDDLDDV
                     YARLSAEGIDFHTPPMLAEEGQIAGSKFCVMRDPDGKTIELVEPGPGMKVPALHAAAR
                     RGVALDDPLIMDG"
     gene            complement(14497..15060)
                     /locus_tag="AC16QF_07860"
     CDS             complement(14497..15060)
                     /locus_tag="AC16QF_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448511.1"
                     /translation="MRIEPLKGWHRYTELMNAETDPRRKAMLDNMRHHLKYECLGDPA
                     IFDSMVPDPEYRFYASFDNAVLTGMEEVREFYYNIWNTRSSLVELAIHRCAAADWGVA
                     CEGEWYQQVPGETLIANGQQVEDRDAWYLSHAHLSWFFPFREIDGRMLLEGEICYIDE
                     PGSTLQKLDPADVITLEEAKASWAALP"
     gene            complement(15153..15824)
                     /locus_tag="AC16QF_07865"
     CDS             complement(15153..15824)
                     /locus_tag="AC16QF_07865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR6448512.1"
                     /translation="MLVAAIAATFAGSAALAGLVFWPAGTFAWPRAWGYLALVGANFV
                     VNLVYLQRVNPSLIAARARFGAGTKRWDVIWSVCFAPAFCSIYVVAGLDAVRYEWTAM
                     SDRLWWLGLAVFVPGTSLFSWSMGVNPFFEKTVRIQTERGHRVVDAGPYQIIRHPGYV
                     GFFGWCFSAPLLLGSWWAFVPATISALGMVVRTALEDRTLQRELPGYADYARRVRYRL
                     LPGVW"
     gene            16112..>16454
                     /locus_tag="AC16QF_07870"
     CDS             16112..>16454
                     /locus_tag="AC16QF_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448513.1"
                     /translation="MSRKRTGHRGSQPYSSDPQTERSLRHSIKDGVYFSAMTGGAESY
                     FSAFAIHLNASTTIIGLLASLPPLLASFMQMLSAWLGRRTGRRKPIIVAGALLQAALL
                     VPIAAVPLLWPD"
BASE COUNT         2757 a         5416 c         5557 g         2724 t
ORIGIN      
        1 tggacccgcg ggaggtcgtg gacgcggcgg tccgggccca cgcggagggc cgcgccccga
       61 tcaacgccgt cgagggcttc gtgcgccagg tcgtcggctg gcgggagttc gtgcgcggcg
      121 tcttccatca ctactacgag ccgatgcaaa gccgcaacat ctggaacgcc gaccggcggc
      181 tcaccgatgc gtggtatcga ggcaccacgg gaatcgcccc gctcgatcac gtgatcggaa
      241 aagcgcttcg gctcggctgg gcacaccaca tcgagcggct gatggtggcg gccaacctga
      301 tgaacctctc cggcatcgag ccccaggagg tgtaccgctg gttcatggaa atgttcgtcg
      361 acgcctacga ctgggtgatg gtccccaacg tgttcggcat gggtctgacc agcgagggag
      421 gcatcttcac cacgaagcca tacatctgcg gctcgaacta cgtcatgaag atgggcgact
      481 tcaagcgcgg cgagtggtgc gacgtgatgg acggtctgct ctggcggttc gtcgcccatc
      541 acgaggagac cctgaaggcc aaccatcgcc ttgcgcccat ggtcgccaac ctgccgcggg
      601 tcgtgcgcaa gcggccggag atctttgcgc tggcagacga gttcatcgag accaccaccc
      661 gcgctgcgtg aggctggccc agatcaactg agcatcggga ggcaaacatg tcagatccgg
      721 aagcggcagt aatgtccata ggggccgtca ccaaggcgac gggcatctcc acccacaccc
      781 tgcggaagtg ggaaagccgc tacggcgtca tcgagcctct gcgcactccc accggccggc
      841 gggtcttcac gcagtcacag gtcgaccggc tcatcctcct gcgggacctg ctggcccagg
      901 gccaccagat cgggcgattg agcaccctgg cggatgatga gctggaggcg ctggccagcg
      961 ccacgccggc tcgcagcagc gtccccgagt tcggcctggt taccgtcgtg ggtccgttga
     1021 tctcggcggt gctttcccgc gaggcgctgg gaccggcacc gttccgcctg gtcacccggc
     1081 ctgcgaccga atggttggca ggcggtgagg aaggctcgga tcaggaggct caggctctgg
     1141 tcgtggagct gtcgacgttg agcgaagaat cgtgcgggag gcttctcgag ctccggcgag
     1201 gcgtgtacca gcgcgtgatc gtcgtctacg gttttgcagc ccgcaccacc ctgcagatgt
     1261 tgctcgatgg gggcatcgtc tgcctcaagg cgcccgttga cggccaggag gtgctgcggg
     1321 cccttgccca gagcgaagcg cccggagtgg tggcggccct cgccgacccg acgattcccg
     1381 cacctcgatt ctcgccggac agcattgccc ggctcgctgc catggcgccc gccatccgct
     1441 gtgagtgccc caatcacatc gcccagctgc tcacggacat cagcgccttc gaggagtaca
     1501 gcaagctctg cgagcagggc gacccggcag accgggtgct gcatgcccgg ctgagactca
     1561 tcgctgcgaa cgcccgtgcg ctcttcgagg aagccatgga gaacgtcgcg agagccgaag
     1621 gcctggagct cgaagagctg gacggctgaa gagctgcgga gctgagcttc agccgccggg
     1681 cgcgctgctc tcgaagctga tgctcttgat gagcgcatag acgggcatgc cttcccgaag
     1741 ccccagatcc tccacggccg ccagcgtgag ccgcgatcgg agatgggctc ccctgagctc
     1801 gacgaaagcc tcggcggtgc ccgacgcttc ctcgatgagc agccgggata tgcgacccgg
     1861 cagcacgttg cggatgctca gaccctctgg ctgggtggtg gccagggcga cgtcccgggc
     1921 ccgtatgcgc acgcggaccg cgtggccttc cgccacgccg ctgagcagcg gaagggtgag
     1981 gacgtcgccg cccacgtcga cgtgggtgac gtgcaggcga gggtcgtgcc cggtcaccgt
     2041 gccctcgagc agaacgccgg cctcgaagcg gcccatcagc ggttccaggt cgagccgctc
     2101 gacgatgtcg gccgttgcgc cgaacgcccg ctcgctgccg ccggccagaa cgagcacgcg
     2161 gtcgcacagc tgagccacct cgtcgacgtc gtggctcacg tagagcgtcg ggatgccgaa
     2221 gccccgctgg atctgatcga ggtacgggag gatgtcggct ttgcgttcgc ggtccagcgc
     2281 tgccagaggc tcgtcgagca gcagcagcct gggcgaggcg agcagggtgc gccccagcgc
     2341 cacccgctgg cgctcgcccc cggagagcga atcgacctgg cgcgtgagga ggggacccag
     2401 atccagcgcg gccaccacct cgggctgctc gatgccgttg cctggccgcc gctgacgtcg
     2461 cacggcgaag tccaggttcc cggccacgtt caggtgcggg aatagcctcg cgtcctgaaa
     2521 catcatgccc accgggcgcc ggtgtggcgg gaggtgcacg ttggcggcct tgtcgaacca
     2581 gacatccccg ccgcaggaga ccctgccgga cgcgggcctg atgaagccgg ccagcactct
     2641 gagcagggtg ctcttgccgc tgccgctcgg tccgaacacg gcggtgaccc cctgtagcgg
     2701 cagcgtccgc ttcaggcgca ggtcgaaacc gggcagcgat gccgtgatct cgatgtccag
     2761 ctcgtcaacc gacattgagc ggggacctcc ggtttagacc gtaaacggtc aggagaacga
     2821 cgaagctgaa ggccaccaac ccggcggaga gcagatgggc ctcggcgtac cgcagggtct
     2881 ccacgtgctc gtagatggcg atggagatca ccttggtctc gcccgggatg ttgccgccca
     2941 ccatcaggac cacgccgaac tccccgacgg tgtgggcgaa agacaaggcg gcggccgtga
     3001 cgaacccccg cttggccaga ggcgcggcga cgcaacggaa cgcgtcgaac gggccggcgc
     3061 ccaggctggc ggcggcctcc attggccccg ggccgatggc ggaaaaggcg ttcgtcagcg
     3121 gctggaccat gaagggcagc gagtagatga ccgatgccac cacgagtcct gcgaacgaga
     3181 aggtcagcgc ctcaccggtt acctgtaccc agaagcttcc gaacggcgag gtcgggctga
     3241 gcatgacgag aaggtaaaag ccgagcacgg taggtggcag caccagtggg agcgcggtga
     3301 gagcttccac gggggctcgc agcggcgagc gtgtgcgcgc cagccaccag gcgatgggcg
     3361 tgccgatgac cagcaggatg agcgtggtcg ccgtcgccag atgcagagtc agccacagtg
     3421 gcgcgaggtc gggcatctac ggcaatccgt acccggccgt ggccatcgcg ccgcgggctg
     3481 cttccgtgcg cagaaacccg aggaaccccg ttgccgccgc attgtccgcc gcccgccgga
     3541 gaagcacggc ctgctgctcg attggcaggt gcatgcccac gggcacgttc cagtggctgc
     3601 ccgggcggcc gatcacctgg gcgcgggcaa gaaaaccggc atcggcgttg ccggtatcga
     3661 ccatggcgtg ggcctggccg atgttttccg cgaacaccat tttatcggcc agctgagcct
     3721 gcaggccgag gtgctccagc acctggcgcg cggcggttcc gtagggcgcc agatccgggt
     3781 tggcaagggc gagccggcgc agacggccgg cgcgcagata gcctaccgga tcctcgccga
     3841 aggcatccgg gcgtgggctc cagagggtga gcagacccac ggcataggtg aatcgcgatc
     3901 cgggcaccgc gcgaccttcg agctccaggc gctcgggtcg ccaggcgtcg gcggcgagca
     3961 gcaggtcgaa gggcgctccc tgcacgacct gagcatatag ctttccggta gcgccggccg
     4021 ctatggaaac cgtgtgcccg gtggcgcgtt cgaactcggc ggccagcagc tcggcgggca
     4081 cgacgaagtt gctggctact gccgccagcg cccgctcggc ccaggcgttg cccgccccgg
     4141 caagcagcca gcacgtggcc agcagctgca gcaggcggct tcgcttccga gctgagctca
     4201 tggactctcc ctggttcccg atgggtgggt tcatagcaca ttcggcccgg aataaggagg
     4261 ccgccgggcg ccattgcatg ttgcggtgaa ggagaatgct tcaggcgccg cgctgacgcc
     4321 ggctgtcgat gtagcgctgc agcctggcga cgcgctgggc gaatgcctcg tcaccggata
     4381 ttcgcttcag ggcccggctg gccgcctcat cgtcgccccg gttcagggca acaaccgcct
     4441 tcagatagat cagtcgcggt ggaggctctt cgccgtaggc ggcgagcgcc gcgtcgaggt
     4501 gtctatcggc ttgcgggtag tcctcccgct gcaggtatag ctgcccgagc agcgtctgtg
     4561 cctcgccgtc gtcccgcatg gcggaagcgg ttttgagtgt ccccacggcg gcgtcgaact
     4621 cccgggccat gatccacagc tcagccagca gcatcacgtc ctcgtagccg ggaagctcct
     4681 gcggaacctc gggcttctcc gagccgtcgg gttcggacgg cgctcccggc agctcggccg
     4741 ccgtccgggc gcgtgtcatg gccgcatcca ggacgtaggc tgcatcggcg ggcatgtcga
     4801 ggctggcgaa cagcctcgcc agactgcgac tgtcgctgag ccccaggggc tccagctcgg
     4861 caagcacggt gaggccggcc gtgccccttg ggacgtcgtc gatcagcagg tagatcgaac
     4921 ccagctggcg ccaccagcgg ctctgctggg gctgcgaggc gatgagcgtt tccagaaggc
     4981 cgatggcgtc ggcgggacga tcgagccggg tgtagacgta ggcgaggatc tcgaaccact
     5041 ggggcctcgg cttgatcgaa gtggcgatcg ccgtctccag ggctttcgcc gcgggctcga
     5101 actcgtccag ctgcagatgg gcgtagccga ccaggaacag acccgccggg tccaggggat
     5161 cggcatggcg cgcccagagc tcgagatggc ccagcgccgt tctggcgtcg tctttcagga
     5221 gccagacctg gcccagcagg aaacgcagct cgggggcgta gcccgggtct ttgccggtca
     5281 ccgccgccag catctcctcg gcggccaggt ccagacgttc ggtctgcatg agcatggccg
     5341 cacgctgccg gacggcgtac cgcagctccg tgggccggtt gcggttggcg cgcatcatct
     5401 cggccagacg ctccagggcc cactcggggt cctcctccgc ccggtccatg acgcgggcga
     5461 gctgctgggc aaacgctggg gacggcacat cctgggcggc gaggcgcgat gccggcagca
     5521 gggccagcat caccagcatc gtcaacgccg cgcccttcac tccggcagct cgaagtcgat
     5581 gcggatgcgg gctcggatag gcgtgggctg gccgttgcgg acgacggggc ggaaacgcca
     5641 ccttatggcc gcggccttgg cggcggcgtc gaacgtggtt cccggtctgg actcgaggat
     5701 ggtcgggtcc accacgccgc cctcttcggt gacgatgaag accatatcca cccacccctc
     5761 gacgccgcgc atcagcgccg aaggcgggta ctggggcggc agcatgctca agggcgtcag
     5821 gtcgctggcc atgaccagct ccaaagcggc gccgctgccc tggaccaggc gctcacccag
     5881 cgccaccccg ccgccttcgc ccagcaggct cttgatgttg aatgccagcg tttccgtagg
     5941 caggtcggcc gcacggttga ggttggtctg caggtcggcg cgaacgcgct tcatttcctg
     6001 gggctttggc ggcggtttgc gccgccggtc cttggtacgt gagcggtcct ccatctcggt
     6061 gcggacgaac tcgatggact gggcgtccac ggggctgagc agacgggtgc ggtcacgccc
     6121 caccatgcgg tccatcatga cgtagagcga cagggtgacg gccagcgcgc cgaggaagat
     6181 gaaaccctgg cgcagcgcca tcactgctcc tcgggctctg cagccgccag cgccaccctg
     6241 cctgccccgg ccaggcgcac ctggtcgatg acctccacga ccaggcccgt gcgtgactcc
     6301 tcgtccgaca gcaccaccac ggccgcgtcc ggagcctcga tgaacatgcg ctccaccacg
     6361 ccgcgaacgc gccgcttgtc cactttcttc ttgtcgatcc agatctcgcc ggcatcggtg
     6421 acggcgatga ggatcgtgct cgccggctgc aggctcgtgg tcttggccgt gggctggtcc
     6481 acggacacgc cggtgatctt cacgaacgtg gcgttcaccg cgaaaaagat gagcaggatg
     6541 aacaccatgt cgatcagcgg cgtcaggttg atggccgcgc tgccgccttc ttcccgcgtg
     6601 tgcagcttca cctcccgtcc ccccggtctg caaagccctc gttgaaccgg ccggcgagat
     6661 gatccgcttc ggtggcgatg cgctcttcca gatcgttggc gaagtagatg ccggtgagcg
     6721 ccgcgatcag ccccgccatg gtggtgatca gcgcctggga tatgccctcg gccatgccgc
     6781 gaatgttgcc cgtgccgaac gcggtgagca cgtcgaaggt cttgatcatg ccggtgaccg
     6841 tccccaggag tcccagcagc ggcagcacgg cggtgatgac ctgaatggtg ccgatccagc
     6901 gacgggaatc ctcgagaaac tgcccggtca gcgcggctcg caggcgcttg ttgttcaggg
     6961 gatccgggct cgagtattgc tgccacagcg cgaatttgcg cgagacggct cgtccgcggt
     7021 cgcgccacag gtacacgtag cgatccagga tcatcatcca catgacgaac gtgagcgcca
     7081 tgatcagcac gatgatccag ccgccggcca tgatcaggtc gctgttgccg aaaagatcac
     7141 ccagcatggt cgctgccccg atgcgtgcgg ccctgggcaa agagctcggc cgcggtttca
     7201 tccagggtgg cgatcagcgc gtttgcccgc ccctggacga agctctggat cagcagcagc
     7261 gggatcgcga cggcgagacc cagctgagtg gtgatcaggg cctgggatat gccgcccgac
     7321 atcaggcgcg gatcgccggc gccgaacagg gtgatgacct gaaaggtctc gatcatgccg
     7381 gtcaccgtgc ccagcagccc cagaagaggg ctcacggctg caaagatcgc gagggtgggc
     7441 aacccccggc gcagcttttc gctggcaatg gtgaccacct cgtccatctt tgcgctgagc
     7501 gcctcggcgt cggccagata cgcctcgtcc cttgccagct ctctgagctc cacgatcacg
     7561 tggtcgccgg ccgcagcgga atccgcgagc actcttcgcg tccgctgcct ggcgaccccg
     7621 atggtcacca ggcgctccac gcccagcagc aggcccagcg cgccgagcgc gagtatgatg
     7681 tagccgatga cgccgccctg ctcgatgcgc tcgcgcacgt cgggcgtctg cacgatcagc
     7741 gagagaatgg agccccggga agggtcgatg gcaactctct gcagcggttc ccgggcttcc
     7801 tcgaatgcca gcacgtcgcc cacgtcgacc cccgccggtt ggcggccgag ggccaggagg
     7861 cgcccggtct ccggcaggta gcgcagatag ctgccgtcgg ccagggcggt gaaagtgccg
     7921 atgcgcgtga cgcggcgcgt gcgctccgcg ccgtcggcgg agatcacggg cgcttcgaac
     7981 cgggacaccc ggccgctgtg attcatctcc tccagcagga tcatccagag ctcgcgcagc
     8041 tcttcggcgg cggggatggc tccgggctcc gccagcctcc tcagcagagc gttgcgcgga
     8101 ccctgctctg ccgagaccag tgagctttcg accattggag ccacgtcccc ggtgatctgg
     8161 cgcaccaccg tgaacagctc ccggagatcg ccggcgttct cgtcgagacg ggtttccaga
     8221 tcggccagct cctgctctga gccctcgaaa tcagcccgca gccggtctgc ttcccgctcg
     8281 gcttcgcggc ggcggtcgcg ggcatcctcc agcaggcgct cgcgctcgtt gcgggctgcc
     8341 acgaaccgtg cctcccgctc ctgcagtcgc ttggcctgca gggcggacga gtccttcagg
     8401 gagtcgatga gctcgtccag ggtggtgggg gctgcggccc agctcgcggg cgcgagcagg
     8461 gccagcagcg tgatcgccgt ccgcctcatg attcgcctcc cgtcgtccac atgggaaggt
     8521 ccatcaggtt tggcggagcc tgcttgcgtg ccatccggac ggcgaggtcg agatcgtcca
     8581 gataattttc cgccaggggc gtccagcgct tctcggctgg gttccagatg ccgccctcgg
     8641 cccggtccag ggaaaggtag tacaacccga tgcggccgac ccgaagaaag tcgacggtca
     8701 gttcccggtc gtcgatgacg atgggtcctt cgtacgcctc gatggtctgc ccgtattccg
     8761 cctcgatctg atacgcctcg atgatacggc ggtagcgctc ggccaggtct acgtcggaac
     8821 gcgtgaggtt ccttcgcagc gactccaccc gggacgtgcg ctcgtcgatc aggagcggct
     8881 ggtccagcgc gacgaagctg tcgaggacgt cgatcatctg caccattagg gggacgatct
     8941 cctgacggac gatctctatc ccggcaagct cggcattgag tcgttccttc tcggcgcgct
     9001 ggctggccag cagctcccgc aggttgtcgt tgtacgtgcg aaggtcctcg tagcgatcga
     9061 cctcgccgtt gtactgggag agcatggcca gggccgcgtc gtcgagatcc tcgacccggg
     9121 cctggctgcg gcctgcaatg gtctgatagg actgcgcctg gtccagagcg tcgcctgcgg
     9181 tagcggtcgg cgcctcgctc cgcgacggat tcggcgtcag cagaaaaagg gtcagccaga
     9241 cccagctcgc gtgtcgtttg ccgtatggct tcacgaggac ccctcccacg tttacacgat
     9301 tttgaggata tagggcgcga atgctttggt tatccgtgaa tccccgtaat ggcgagttgt
     9361 cggcgcatag cgagcgcggg tatcgtttgc agcgggagag gtggaaggca catgttgagc
     9421 agatccgagg ccttggaggc gctgaccgcg ccgggtcaac cctacgagct cgtggacgtc
     9481 gagctgtacg gtcgcagctg ccgtgccttt cgcaacgcgc cgccccttct tcgggatcta
     9541 tacgcgtcca ccgccagcga cctgccgttc atcgtctatg gcggcgagcg cctttccttt
     9601 gcggacgcct gggcagagtc ctgccgaatc gccatcctgc tgatggaaca gtacggggtc
     9661 gccaggggcg atagggtcgc catctccatg cgcaactacc cggagtggat gacggccttt
     9721 gccgcggtca cgtccatcgg cgccatcgcg gtggccatga acgcgctctg gacgccggag
     9781 gaaatggagt acggcctgaa ggacagcggc gccaaggtcc tcctggcaga cgaagaacgc
     9841 cttgcccgat tggcgctttg tgccgatccg ccggcgggtc tagccgttct ggcggtgcgg
     9901 gccgccgatg acgcggggac cggcgcccgg gatctgcgag aggccctcgc cggggtcccg
     9961 gcaggcaaca tgccggcggt ggacgtcgcc ccggaggacg atgccaccat tttgtatacc
    10021 tccggctcca cgggacaccc caaaggcgcg gtctcctgtc accgcagcct tgtcaccgcg
    10081 ctcctttcct gggaactcga cgcgcacgcg ggcgcctaca tgcagggcgt cgatctgccg
    10141 gctcccgagt ctcagccggc cacgctgctc gccgtgccgc tgtttcacgc gacgggctcc
    10201 catgcggtct atcttgcctc ctaccggtct cagcggcggg tcgtttccat gtacaagtgg
    10261 gacgtcgagg aggctgccga gctcatcgag cgcgagcgca tctcgtcttt cgtcgctccg
    10321 ccggccatga cgggggatct cgtccagttt gccgcacgca ccaaccggga tctgagctgc
    10381 ctggatcggg tcggcggcgg cggtgcggcc cgtgcgcccg agcaggtgcg cgggatccga
    10441 cgcacctttg ccaaggccat gccgagcacg ggctggggca tgacggagac caacgccatc
    10501 ggcactgtca tctccggtca ggaatacctg gatcgcccgg aaagctcggg ccgctgcagc
    10561 gccgtgctgg aattgcgggt ggtagatccc gagggtcgcg agctgccctg cggagaacgc
    10621 ggcgagctgc aggttcgcgg cacctcgctc atccgcggtt actggaatcg cccggaggcc
    10681 actgcggagg cgatcgtcga agggtggctt cgcaccgggg atgtggccta tctggatgac
    10741 gacggttacc tcttcatcgt cgaccgtatc aaggacctgg tcatacgggg cggtgagaac
    10801 atcggctgcg gcgccgtgga ggcggcgttg caggaacacc cggacgtgat agaggcgtgc
    10861 gtttacgcgg tgcccgacga gcgcctgggg gaggaagtcg gcgcgaccgt ctacatctgc
    10921 cgcccggtcg acgaggagga gatcaggaac tttctggctc cccgcctggc acgcttcgag
    10981 atcccccgct atctgtcttt ttccacccag ccgctgccgc gcaccgcatc gggcaagatc
    11041 ttcaagcgcc aactcaagca ggaggcagcg gaccggctca ctggctgaac tcatcgcatt
    11101 actcgcaaag gaggcatatc acatgaaact cggcattctc ggcggcaaca tcggccgcaa
    11161 gacgggcatc aacatcgagg cgattcgcaa ggccgagtcc cttggctacg actcggtctg
    11221 gacggccgaa gcctggggag gcgacgcggt cacgcccgcc acctggattc tcgcgcagac
    11281 cgagaagatc aaggtgggca ccgccatcat gcagatgcct gctcggacgc cggccatggc
    11341 cgccatgacg gccatgagcc tggcggagct ctccggtggc cggttcatca tcggcctcgg
    11401 tgcttcgggg ccccaggtag tggaaggctg gcacggcgtg ccgtacggca agcccatgac
    11461 gcggctgaga gagtacgtgg ccatcatgcg caagattttc gcccgtgagg acaagctcac
    11521 cttcgatggc gagatgtacc agatccctta cacgggcccc ggctccacgg gtctgggcaa
    11581 accgctgaag agcatcctgc actgcgagga ggacatcaga atcttcgccg cgagcatcac
    11641 gccgaagggt gtggagactg ccgcggaggt tgccgacggg ttcttcccga tctggatgga
    11701 tccggaaaag ttctccgtgt tcgaggagcc catacggaaa ggttttgccg cagctggcga
    11761 caaagatctg agcagtttcg aggtgtcgcc gttcgtcacc gtggtcatcg gggacgacgt
    11821 ggagcagtgc atggcgccca tccgtggaaa catggcgctc tacatcggcg ggatgggagc
    11881 ccgcaacaag aacttctaca acgactacgc caaagcgctc gggttcgaag acgccgccgt
    11941 cgagatccag gatcatttcc tggccggacg gcgcgaagag gccatgcgcg cggtgccggc
    12001 ggagctgatc gacgcctgcc acctggtggg tccggccgac cgcattcggg aaaggctgca
    12061 gagatggaag gcggcgggcg gcaacggtca ggtggcgagc atgctgctcg gcacccagca
    12121 gcccgaagcg ctggaactgg tcgcctcgga gatgctctag gggcgctcag gacgaccttc
    12181 acaggaaaag gagacaacag caatgagtaa cggcaccatg ccgatctttc agcacgcgta
    12241 cttcgtcaac gacatcgagg aagccgctca ccggtggaac cggctctacg gagccgggcc
    12301 tttcgtgctc gtgccccacc acaaaacgga caagttccag tatcggggca cggaccagga
    12361 agcggacgta tcctacgggt tcggctacct gggcgaaaac cagatccagt tcatccagca
    12421 gcacgacgaa accccgtcga tctatcggga catgtatcag gcgggggaag agggcttcca
    12481 tcacgtcggc tgtcttgtca acgacttcgc tgccgcccgt cagcgcatgc tggatctggg
    12541 tttcgtgaac gcctgcgagc tctgggcgga cgacgttaac gccgcgtatt tcgatacgcg
    12601 ctcggtgaac ggcggcttca ccgagatcca cggagatccg gtgcacatcc tgggtacttt
    12661 tgcgacctgg aagcgggcgc atcagaacct gaagcccggc gacagcccca tcatggacat
    12721 gtcggcaccg ttcccggacg gcagggatcc cgggtatctg gactgaagcg ccgtgaagcc
    12781 ccaacctttc cgcatcgagg tcccggaaga gacgctgcgc gatctggccg accggctcga
    12841 tcgttccggc tggcccgacg acttcgcgaa cgatgactgg tcctacggaa ccaacggagc
    12901 ctacctgcgg gacctggtcg ggttctggcg acgggactac gactggcgcg cggtggagcg
    12961 caagatcaat gctttcgatc actaccgggt agagatcgag ggcatgcccg tgcattttct
    13021 gcgcgagccg gggcgcggcc cgaacccggt gcccctgata ctcagccacg gctggccctg
    13081 gacgttctgg gacctcaacg aggtgatccg ccccctggcc gatccggcgt cgtttggcgg
    13141 agatcccgag gatgcgttcg aggtcatcgt gccatcgctg cccggcttcg gcttctcgac
    13201 cccgctcacg acccctggga tcaacttctg gaggacggcc gatctctggc acacgctgat
    13261 gaccgaggtg ctcggctttc agcgcttcgg cgcccagggc ggcgactggg gcgcgctggt
    13321 gactgcccag ctgggccaca agtacgccga aagcctcttc gggatccacc tgacgaacct
    13381 gatcccgctg acgctcttca atcatcagcg tccgtgggac atcaccgcgg gcgtgatggt
    13441 tcccgatgac gttcccgcgg acgagcgcga ccggcagctc gctcagcagc ggcgcatcgc
    13501 cagccacgtg gcggtgcagg tgctcgatcc gcagacgctg gcgtacgggc tgcacgactc
    13561 ccccgtgggg ctgctggcgt ggctgctgga acgccgctac ttctggggcg actgcaacgg
    13621 cgacgtggaa tcccgcttca gccgcgagca cctgatcacc acggcgatga tctactgggt
    13681 aacgtccagc ttcgccacgt ctgcccgata ctacgccgag gccgcgcgca atccgtggcg
    13741 gccgagccac gaccgctcgc cccaggtgga ggcgccggcg ggggccacct acctgggagg
    13801 ggacgtgggt cgtggaacga ttccggccgt gcgggaggcg ttcgacctgc gctacgagaa
    13861 ctcacatccc gtgggcggcc acttcgcccc gatggaggag ccggaggtcg tgatcaacga
    13921 catccgggag acgttccgcc cgttgcggtg actttcgcgc ggtcgatggc ggggacggct
    13981 agccgtccat gatcagcggg tcgtccaggg cgacgcctcg ccgggcggct gcgtgcaggg
    14041 cgggaacctt catgccgggc ccgggctcca ccagctcgat ggtcttgccg tcggggtcgc
    14101 gcatcacgca gaacttggag ccggcgatct gaccttcctc ggccagcatg ggcggcgtgt
    14161 gaaagtcgat gccctcggcg ctgagacggg cgtagacatc gtcgagatcg tccacggcga
    14221 agcagatgta aacatagccg acgtcatcct ggcgcatgtc catctgccgg cgggtcggtt
    14281 cgaactccag cagctctatg gcgaaatcgc cgacgggcag cagcgcggcg cgatgtatgt
    14341 ggggcggaag ccgcagcacc tgctcgccgg tggggcctga aacttccagc gctaccccgg
    14401 gcttcagccc gaacaccttg ctgtaccact caacggcgcg ctccagattg ctcaccgtga
    14461 tgccgacgtg gtgaaaggtt gctgatatcg gcagggtcat gggagcgcgg cccaggaggc
    14521 cttcgcctct tccagcgtga tcacgtcggc gggatcgagt ttctgcagcg tgctgcccgg
    14581 ctcgtcgatg tagcagatct cgccttccag cagcatgcgc ccgtcgatct cacggaacgg
    14641 aaagaaccag gaaaggtgcg cgtgagacag ataccaggcg tcacgatctt ccacctgctg
    14701 gccgttggcg atgagcgttt cacccggcac ctgctggtac cactcgccct cgcaggccac
    14761 gccccagtcc gcggccgcgc accggtggat ggcgagctcc accaggctgc tgcgcgtgtt
    14821 ccagatgttg tagtagaact cccggacctc ttccatgccg gtgagcacgg cgttgtcgaa
    14881 gctcgcgtag aaacggtatt cggggtcggg aaccatggaa tcgaagatcg cagggtcgcc
    14941 gaggcattcg tatttcaggt ggtgccgcat gttgtcgagc atggccttgc ggcgcgggtc
    15001 ggtctccgcg ttcatcagct ccgtgtagcg gtgccagccc ttcagcggtt cgatgcgcat
    15061 ctcgtctccc cattcttaac gatcgagctg cttaagttat cacgatttgt cggcgtctct
    15121 gcctgccgga ggccggcgtc gggcgatggt cctcaccaga cgcccgggag caggcggtaa
    15181 cggacgcgcc gggcgtagtc ggcgtagccg ggaagctccc gctgcagcgt ccggtcctcc
    15241 agggccgttc gcaccaccat gccgagtgct gagatggtgg cgggaacgaa cgcccaccag
    15301 gaacccagca gcaggggggc cgagaagcac cagccgaaga agccgacgta gccgggatgc
    15361 cggatgatct ggtaagggcc ggcgtcgacg acccggtggc ctcgctccgt ctggatccgg
    15421 acggtcttct cgaagaaagg gttgacgccc attgaccagc tgaacagtga cgtccccggc
    15481 acaaacacgg cgagacccag ccaccacagc cggtcgctca tggccgtcca ctcgtagcga
    15541 acggcgtcca agcccgcgac gacgtagatc gagcagaacg ccggcgcgaa gcacacgctc
    15601 cagatcacgt cccagcgttt tgtgccggcg ccgaaacgcg cgcgcgccgc gatcagggag
    15661 ggattcacgc gctgcagata cacgagattg acgacgaaat tggcgcccac gagcgcaagg
    15721 tagccccacg cccggggcca ggcgaaggtg ccggcaggcc agaagacgag gcccgccagc
    15781 gccgcgctgc cggcgaacgt ggcggcgatc gcggcgacga gcactttccc gatctgccct
    15841 ggggaccctg ctggaactgg atggtttgcc aagcgctcga tatcaagccg gtgatcgatc
    15901 gggcccgacg ttaacccagc tgccggccat agccaaaccc atctccctgg aagtcgacgg
    15961 cccgtgcgag gacgtcccgc cgacgcgcga tgatggtgtc gcgagcagcg ctggggtaat
    16021 ctgcgccgcg ccagtttcag agtatcgtga tggttcgcgc attgttcgcc cgggagctcc
    16081 gggtttaggg ccggttggcg ggaagcggac attgtcacga aaaagaaccg gccaccgcgg
    16141 gagccaacct tactcgtccg atccccagac cgagcggtcg ctgcgccatt ccatcaagga
    16201 cggcgtttac ttctccgcca tgaccggggg ggcggagagc tacttctcgg cattcgccat
    16261 tcatctcaat gccagcacga cgatcatcgg gttgctcgcc tcgctgccac cgctgctcgc
    16321 atcattcatg cagatgcttt ccgcctggct cggccgccgc accggccgcc gcaagccgat
    16381 catcgtagcg ggtgccctcc tgcaggcggc gctgcttgtg cccatcgcgg cggtcccgct
    16441 gctctggccc gatg
//
LOCUS       DBNHLM010000172         3606 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1461153, whole genome shotgun sequence.
ACCESSION   DBNHLM010000172 DBNHLM010000000
VERSION     DBNHLM010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1461153"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="AC16QF_07875"
     CDS             complement(<1..727)
                     /locus_tag="AC16QF_07875"
                     /inference="COORDINATES: protein motif:HMM:NF044765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448514.1"
                     /translation="MASSIVPQLAANSPPPPDYYAENLRQLITHVHAHYGDVLRREEI
                     GYGRRIVACSVPAQRLYARLLSRRGPWIRIDKLRYREVSDVTAAVGELEEAGLVVRNG
                     RAPADALLGLLSTAERRELFAAIGDAPKARWINQCVARYSDAHVLSRLAGVHPWVSIR
                     DFPTFWLHQLLFFGDTRQDLSAFVLQDLGLVRYEPYELDPRHRQFANRERLDEYLRYL
                     ELSSLSYRLEEQPVLASWLSGAMW"
     gene            800..1246
                     /locus_tag="AC16QF_07880"
     CDS             800..1246
                     /locus_tag="AC16QF_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448515.1"
                     /translation="MPAFSYCICTTVDARALQALLEASGQGSYKDEQPWLIAREILEE
                     AASQSARLPILFASGHPSEFSHWGFIESIDVVELHRGTWESRCAFSPLQPVNPIWTSI
                     DSVFLKPTGEQLRREALEGIAHHRYPLNEGELHPYAICETPAFILA"
     gene            complement(1277..1501)
                     /gene="csrA"
                     /locus_tag="AC16QF_07885"
     CDS             complement(1277..1501)
                     /gene="csrA"
                     /locus_tag="AC16QF_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HIR6448516.1"
                     /translation="MLVLTRRAGESIMIDDDIELKVLKIRGSQVHLGIDAPKRSAVHR
                     KEVWLRINGEPKAAGREDKEDSSDPSRGVA"
     gene            complement(1615..2916)
                     /locus_tag="AC16QF_07890"
     CDS             complement(1615..2916)
                     /locus_tag="AC16QF_07890"
                     /inference="COORDINATES: protein motif:HMM:NF014680.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR6448517.1"
                     /translation="MQARASYAAGQLQQAEAGQAERLVLEERLGERERDLVRLEERIT
                     SFENQLNSARSALDERTKELSDRQAAQAAVEARLEESRKGFAEKEAVFKESSEALKQE
                     FELLATKIFDQQGVKHQERLSSVLTPFKEQIVDFRKRVEQVYHTDTKDRASLLNEVRN
                     LQKASERINEEAENLTKALKGDVKVQGNWGEVVLERVLEESGLREGHEYFLQAARRSE
                     RGELKRPDVLIRLPDGKDVVVDAKVSLLAYEEALSAETDGARELALRQHLVSLRAHVK
                     RLGEQDYDQLSDVRSLDFVLLFVPIEAAFTMAMEHDHRLFTEAFEKRIVIVSPTTLMM
                     TLRIIHNVWRFEKQNRNAQDIARRAGALYDKLRGLIEDMDLLGKQLGTVEKTYQAAYG
                     KLASGKGNLVRQVERFRELGAQVKRPLPKSLLDEATDRSDD"
     gene            complement(2994..>3606)
                     /locus_tag="AC16QF_07895"
     CDS             complement(2994..>3606)
                     /locus_tag="AC16QF_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6448518.1"
                     /translation="EHNFKARLDGNDDRGDDATPWVRLLRQSKPIIAAVNGASVGVGA
                     TMILPADIIVAADSAKFAMGFIKMGLVPELASSHFLVQRMGFGRASEMCLTGKMYPAS
                     EAFAMGLVDHLVADDALMDKAMGLAQAIAANPGPQLRWVKQLLTENGCATDLDEVQRL
                     EGKLLQQAFASPEHKEAVAAFLDKRTPDFIGARAAQGEAGGTA"
BASE COUNT          640 a         1172 c         1098 g          696 t
ORIGIN      
        1 tccacatcgc cccggagagc catgatgcga gcacaggctg ctcttccagg cgataggaaa
       61 ggctgctgag ctcgagatac cgcaggtatt cgtcgagccg ttcacggttg gcgaactgcc
      121 gatgccgggg atcgagctcg tagggctcgt accggacgag gcccaggtcc tggagaacga
      181 aggcgctgag atcctggcgg gtatcaccga agaacagcag ctgatgcagc cagaaggtgg
      241 gaaagtcgcg aatgctcacc catggatgta caccggcgag ccgagagaga acgtgagcat
      301 cgctgtaacg cgctacgcat tggttgatcc agcgagcctt cggcgcatcg ccaatggcgg
      361 caaagagctc acgtctttcc gccgtagaga gcagaccgag cagcgcgtcg gccggtgccc
      421 ggccgttgcg gacgacgagc ccggcttcct ccagctcgcc aaccgcggcc gtcacatcgc
      481 tcacttcgcg atagcgaagc ttgtcgatac gaatccacgg cccacgtcgg ctcagaaggc
      541 gggcatacag ccgctgggcg ggaaccgagc aggccacgat gcggcgcccg tagccgatct
      601 cctctctgcg cagcacgtca ccatagtggg cgtgcacgtg ggtgatcagc tggcgcaggt
      661 tctcagcgta gtagtcgggc ggcggcgggc tgttggcggc aagctgaggc acgatgcttg
      721 aagccatccg gttgcttcga acgaggcctg tggattataa agaggtctgt atacatgtac
      781 agtactcgcc agctccccaa tgcctgcctt ttcctactgc atctgcacca ccgtcgacgc
      841 ccgcgcgctg caggcgctgc tggaagcaag cggtcaaggc agctacaagg acgagcagcc
      901 ctggctcatc gctcgagaga ttctggaaga agccgcgtct cagtcagccc gcctgcccat
      961 ccttttcgcc agcgggcatc cttccgagtt ctcgcactgg ggattcatcg aatccatcga
     1021 cgtggtcgag ctgcaccgcg gaacctggga atcacgatgc gcgttcagcc cgctgcaacc
     1081 cgtaaacccc atctggacga gcatagacag cgtcttcctg aagcccacgg gcgagcagct
     1141 cagacgagag gcgctggaag gaattgcgca ccatcgatat ccgctcaacg aaggcgagct
     1201 gcatccgtac gcgatctgcg agacgccagc gttcatactg gcttaaagga gagtgatcgg
     1261 gttgagctga gtccgctcag gcgacgccgc ggctgggatc tgacgagtct tctttgtctt
     1321 cgcggcctgc cgctttcggc tcgccgttga tgcgcagcca gacttccttt cggtgcacgg
     1381 cagaacgttt cggcgcgtcg attcccagat gcacctggct accgcgaatc ttgagcactt
     1441 tgagctctat atcgtcgtcg atcatgatcg actccccagc acgacgggtg aggaccagca
     1501 tccaggtttc cttgcaatcc ttttctccgt agaaatctac tggcattgac tgatccgttc
     1561 tgtgacgtgc acgcggcttt ggccgggcag cggcgagaaa ggagaactgg ttggctaatc
     1621 gtctgagcgg tcggtcgctt catccagaag ggacttgggc agcggcctct tgacctgggc
     1681 gcccagctca cggaagcgct ccacctgcct gacgaggttg cccttgccgc tggcgagctt
     1741 tccgtaggct gcctggtagg ttttctcgac ggtgccaagc tgcttgccga gcagatccat
     1801 gtcctcgatc agtcctctga gcttgtcgta aagagccccc gcccgccgcg cgatgtcctg
     1861 ggcattccgg ttctgctttt cgaaccgcca gacgttgtga atgatccgaa gcgtcatcat
     1921 cagcgtcgtg gggctcacga tgacgatgcg cttctcgaac gcctccgtga acaggcgatg
     1981 atcgtgctcc atggccatgg tgaaggccgc ctcgatgggc acgaagagaa gaacgaaatc
     2041 cagcgagcgg acgtcgctga gctgatcata gtcctgctca cccagacgct tcacatgagc
     2101 gcgaaggctg accaggtgct gacgcagggc aagctcccgg gcgccgtccg tctcggcgct
     2161 caaggcttcc tcgtacgcca ggagagagac cttggcgtcc accaccacgt ctttgccgtc
     2221 gggcaggcgg atcagcacat cgggcctttt cagctctccc cgttccgagc ggcgggcagc
     2281 ctgcagaaag tactcgtgcc cttccctcag ccccgactcc tccagcacgc gctccagcac
     2341 gacctcgccc cagtttccct ggaccttgac gtcacctttg agggccttgg tgaggttctc
     2401 ggcttcttcg ttgatccgct cgctggcttt ctggaggttg cgcacttcat tgagcagcga
     2461 ggcacgatct ttggtgtcgg tgtgatagac ctgctctacc cgcttgcgga aatcgacgat
     2521 ctgctccttg aagggggtga gaacgctgga cagccgctcc tggtgtttga ccccctgctg
     2581 atcgaagatc ttcgtggcga gcagctcgaa ctcctgcttg agcgcttcgc tggattcctt
     2641 gaagacggct tctttctcgg cgaacccctt tcgcgactcc tcgagtcgag cctcgacggc
     2701 cgcctgggcc gcctggcgat cactgagctc cttcgttcgc tcgtccagcg ctgagcgtgc
     2761 cgagttcagc tggttctcga acgaggtgat ccgctcctcc agccggacca gatcccgctc
     2821 acgctcgccc agccgctctt cgagcaccag acgttcggcc tggcccgcct cggcctgctg
     2881 aagctgcccg gcggcatacg aggctcgcgc ctgcacccgg cagcgccaca gccagacgac
     2941 gcttgcgagc gcggcgcccc ccgcgaagag cccgacgccc tggacccaga gatctatgcc
     3001 gtgcctcccg cctccccctg ggcggctctg gccccgatga agtcgggcgt ccgcttatcc
     3061 agaaaagcgg caacggcttc cttgtgctcc gggctggcga acgcctgctg caaaagcttg
     3121 ccctccagcc gctggacctc gtcgagatcg gtggcacacc cgttttcggt cagcagctgc
     3181 ttcacccagc gcagctgcgg gccgggattc gccgcaatgg cctgagcgag acccatggcc
     3241 ttgtccatca gcgcatcgtc ggcgaccaga tgatccacca gccccatggc gaaggcctcg
     3301 ctggccgggt acatttttcc ggtgaggcac atctcgctgg cacggccgaa ccccatgcgc
     3361 tggaccagga agtgggagct cgcaagctcg ggaaccagac ccatcttgat gaaccccatg
     3421 gcaaacttgg cggaatcggc cgccacgatg atgtccgccg gcaggatcat ggtggcgccc
     3481 acgcccacgc tggccccgtt gaccgccgcg atgatgggct tggactggcg cagcaggcgt
     3541 acccagggcg tcgcgtcgtc accgcgatcg tcgttgccgt cgaggcgtgc tttgaaattg
     3601 tgctcg
//
LOCUS       DBNHLM010000173        10980 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_922480, whole genome shotgun sequence.
ACCESSION   DBNHLM010000173 DBNHLM010000000
VERSION     DBNHLM010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10980)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10980
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_922480"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /gene="prfB"
                     /locus_tag="AC16QF_07900"
     CDS             join(<1..40,42..1064)
                     /gene="prfB"
                     /locus_tag="AC16QF_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033384.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR6448519.1"
                     /translation="DLRERDVALRGYLDFAEKEHRLAEVELTLAEPDVWNDPEEAQRL
                     NKERSALEQVVKGLRDLHETLNDLRDLAELASTEEDHDALTEVDEELDDLERQLESLE
                     FRRMFQGEMDEANAYVEIQSGSGGTEAQDWAEMLLRMYLRWGEKHGFDTEITELSPGE
                     VAGIKGATVQFQGDHAFGWLRTETGVHRLVRKSPFDSGNRRHTSFASVFVAPEIDDDI
                     EIDINPADVRVDTYRASGAGGQHVNRTDSAVRLTHLPTNTVVQCQSERSQHQNKDKAF
                     KQLRAKLYELAELERNAERQAVEDSKADIGWGSQIRSYVLDQSRVKDLRTHVERSDPG
                     NVLDGDLDEFIEASLKAGL"
     gene            1077..2564
                     /gene="lysS"
                     /locus_tag="AC16QF_07905"
     CDS             1077..2564
                     /gene="lysS"
                     /locus_tag="AC16QF_07905"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR6448520.1"
                     /translation="MTDESDIIRHRREKLARWRENGRAYDNQFRRDALAQELHARCVD
                     LDKDALADAGIQVAVAGRVMLRRVMGKASFITLKDTSGTIQCYLRTEDVGPEAYEEFD
                     DLWDIGDIVGVRGTMMRTNKGELTVAAASIELLNKTLRPLPEKYHGLTDHEIRYRQRY
                     LDLIMNDDTRRVFEIRSRVIGKIREFMQERGFLEVETPMMQVIPGGATARPFVTHHNA
                     LDLELYLRVAPELFLKRLTVGGMERVFEINRNFRNEGLSTRHNPEFTMLEFYWAYADF
                     EDLIALTEELLQLLARDVLGTGTLTYQGTEIDLARPAERYTMVEAIERHTGIASGQLK
                     DPDALAAILKGMNVQPEADWGIGKMQLELFEARVEDRLVQPTFITHYPTEVSPLSRRN
                     DEDPEVADRFELFIAGREIANGFSELNDPEDQAERFRAQALLRSGGDVEAMHFDDDYI
                     TALEYGLPPTAGEGLGIDRLVMLLTDSPTIRDVLLFPLMRPVQDG"
     gene            2557..3273
                     /gene="ung"
                     /locus_tag="AC16QF_07910"
     CDS             2557..3273
                     /gene="ung"
                     /locus_tag="AC16QF_07910"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR6448521.1"
                     /translation="MVEAARRSEERVRLEPSWKAVLGGEFDKPYMNKLREFLLREKRA
                     GKVIYPRGDNIFAALDLTPLEDVKVVIIGQDPYHGPDQAHGLCFSVQPGIPQPPSLVN
                     IFQEINEELGGPAKRLDDTNGCLEPWARQGVLLLNAVLTVERGRAGSHQGMGWEIFTD
                     KVVDVLNRERDGLVFLLWGSYAQKKGAILDVQRHCVLKAPHPSPLSAHRGFFGCGHFA
                     RANEYLQQRGVAGIDWFDVA"
     gene            3329..4117
                     /locus_tag="AC16QF_07915"
     CDS             3329..4117
                     /locus_tag="AC16QF_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR6448522.1"
                     /translation="MIESPTEKLLVEQRGHTLLVTINTPPANTWDAESLQGLKNVVEQ
                     ANAERSVYAIVITGQGEKFFSAGADLKLFADGDPAVASEMAGQFGSAFETLSAFRGVS
                     IAAVNGFAMGGGLECALACDIRVAEAQAKLALPEATVGLLPCGGGTQNLAWMVGEGWA
                     KRMILCGERVDAATALKIGLVEEVVDAGQSLGRALELAEMVGRQSPPAVAACKDLIQQ
                     ARSGNISKAYSTERAAFVGLFDTQDQKEGVNAFLEKRSPEWVNG"
     gene            4125..5240
                     /locus_tag="AC16QF_07920"
     CDS             4125..5240
                     /locus_tag="AC16QF_07920"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF004127.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6448523.1"
                     /translation="MEPVNSANAPVQLFEHPTESGHVLAEARLNFEGTLNSLSLEMID
                     IMSPALADWSGRAEIVAVLFTGAGDRAFCAGGDIQALYHAMVKNHEHGEVVDDYPFQF
                     FEREYRFDHQIHTFPKPVIAVGHGVVMGGGLGILSAARYRLVTERSRIAMPEVTIGLF
                     PDAGQTWLLRNMPEHFATFLGVTGSHMNGADALMVGIGTHGIEQASRESVRERLCAAS
                     WQADAHAAVQEALADLPAGGFPEAQVERLPDSLSVAGGIADVAASIAALRGGSDWIDR
                     GIGAMQSGCPTTVGIVVEQLRRAGDMSLADTFRMEMTVATHCANNHDFAEGVRALLIE
                     KDNEPAWQFGDLDSLPQAYVDSHFVEPWAQNPLADLN"
     gene            5253..6146
                     /gene="mmsB"
                     /locus_tag="AC16QF_07925"
     CDS             5253..6146
                     /gene="mmsB"
                     /locus_tag="AC16QF_07925"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006574 - L-valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HIR6448524.1"
                     /translation="MSRIGFVGLGNMGGHMARNLVAAGHQLKVFDLVPDLMAAVEGAQ
                     AQESAAAAATSVDIFISMLPAGRHVAGLYGGEDGVIAAVDEGTLLIDCSTIDPQTARS
                     VAAEAGAAGLAMIDAPVSGGTAGAEAGTLSFIVGGEADALERAREVLNVMGSNIFHAG
                     ASGAGQIAKICNNMLLAVLMAGTAEALALGVSNGLDPAVLSEVMKRSSGGNWALNVYN
                     PYPGVMEGAPASRDYEGGFLVDLMTKDLGLAMDTAEQSRSAVPMGSLAKNLYRVHQQL
                     HDAGGLDFSSIQWLFKPDLKG"
     gene            complement(6175..7059)
                     /gene="ygfZ"
                     /locus_tag="AC16QF_07930"
     CDS             complement(6175..7059)
                     /gene="ygfZ"
                     /locus_tag="AC16QF_07930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAF17-like 4Fe-4S cluster assembly/insertion
                     protein YgfZ"
                     /protein_id="HIR6448525.1"
                     /translation="MLRQKWLSQTHPVTGEPDAPAGVCYLPDLSVLRFEGNDAATFLQ
                     GYVTCDTQKLSPKRLQPAAVCNLQGRVVFNGWCVRADDAEKGPVDLIVHRSLAPRVAS
                     FLEAYLRFSRTSLADMGEDILVFGYLANDPPPGALRISEDIGLLLADDLEKAEKIWHD
                     LPHAPTEVWQAHRLAEGWPLISTPTSESFLPQMLDLDRLGAISFDKGCYLGQEIVARA
                     QHRGKVKRRLARLNWTGPEAPAAGTEVTHADDASRACGTLVEAARLSEHGGIAIAVIA
                     EAAPDELRVGECRLSARG"
     gene            7129..7386
                     /locus_tag="AC16QF_07935"
     CDS             7129..7386
                     /locus_tag="AC16QF_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006105 - succinate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0034552 - respiratory chain complex II
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HIR6448526.1"
                     /translation="MEGDASQGRIYWRSRRGLLELELLLIPFVRERFEELPQDDQDRY
                     VRLLEHEDLDIYDWLQGRSKPEDESLHAIVDRIRRHNSSRV"
     gene            7412..7714
                     /locus_tag="AC16QF_07940"
     CDS             7412..7714
                     /locus_tag="AC16QF_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448527.1"
                     /translation="MAGIATLAAAAGWMWLGASGLALVVLWFRARWPLPDVVRYQLDA
                     RRVRLVSLGVVRVRWTEGWFKAGEVFSDEIDPADFAALRRFLKAAVRDRTDQLKTG"
     gene            complement(7696..9315)
                     /gene="nadB"
                     /locus_tag="AC16QF_07945"
     CDS             complement(7696..9315)
                     /gene="nadB"
                     /locus_tag="AC16QF_07945"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016568828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HIR6448528.1"
                     /translation="MGQRFSTDILVIGGGAAGLSAALHLAEHARVTVLCKGDVESAST
                     HWAQGGIAAVTDPDDTYEAHIQDTLNAGAGLCDHSVVAQTVERAPQVISQLVQWGIEF
                     DRSENEYHLTREGGHSHRRILHVADATGKALTQTLTDKVLDHPNIELFTDRVAIDLIN
                     LSKFGRHPNRCAGAYVLNRTTNHVEVFQAHFVILATGGASKVYLYTSNPDSSTGDGIA
                     MAWRAGCRVANMEFNQFHPTCLFHPHAKSFLISEAVRGEGGVLCLPSGERFMERFDPR
                     LELAPRDIVARAIDHEMKRLGLDCVYLDISHRPRSFIKSHFPTIAQRCADFGIDMANE
                     PIPVVPAAHYTCGGVVIDGNGASDVPGLYAVGEVSCSGLHGANRLASNSLLECLVFGE
                     AAARDIKRRLGEPRWDVNVPQWDESQVVDSDEDVIIAHNWEELRRFMWDYVGIVRTDK
                     RLQRAQRRIDLLKQEVHEYYSNYRVSNDLIELRNLIHVAELIVRSALVRKESRGLHYN
                     LDHPETMSRADDTILFPGSMDRLDVLQPVFS"
     gene            9414..9995
                     /gene="rpoE"
                     /locus_tag="AC16QF_07950"
     CDS             9414..9995
                     /gene="rpoE"
                     /locus_tag="AC16QF_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HIR6448529.1"
                     /translation="MAKDTDKELVRRVQKGDRYAFDLLFSRYQHKILNLVSRYLKDPQ
                     DVEDVTQEAFIKAFRALPRFRGESAFYTWLYRIAINTAKNHLVARGRRPPGVDVDMED
                     AEIMDGADALRVSESPEAVLERDELSAAIDVAISQLPDDLRSAVTLREFDGLSYEQIA
                     HIMDCPVGTVRSRIFRARESIDAHIEPLLPRRR"
     gene            10119..10688
                     /locus_tag="AC16QF_07955"
     CDS             10119..10688
                     /locus_tag="AC16QF_07955"
                     /inference="COORDINATES: protein motif:HMM:NF015810.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HIR6448530.1"
                     /translation="MSEKLRESLSATMDGEADEFELRRVLDEMQKDGGLREVWSRYHI
                     AGMLMRGERVHFSSDIRERVWSQLQLGTDDEAVESAALPAAQAGPADGPRSRLGRLAG
                     LAVAASVAFAAVIGVIGLWDEGAGEPPATAGTVTIEGPRVSNPAFTSEISPRDLQRAN
                     AYMMHHFQHQGMNQPGVSSFVKMVTYERR"
     gene            10685..>10980
                     /locus_tag="AC16QF_07960"
     CDS             10685..>10980
                     /locus_tag="AC16QF_07960"
                     /inference="COORDINATES: protein motif:HMM:NF015825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HIR6448531.1"
                     /translation="MRPARFILLVFGVLVSAISVADEDPHAWLADMNRAFSELSYDGI
                     FTYFSGDDLDTLRVVHMVVDEVQRERLVHLNGAPREIVRTGEEVACILMPGDELL"
BASE COUNT         1986 a         3285 c         3715 g         1994 t
ORIGIN      
        1 ggacctccgc gagcgcgacg tcgcgcttag ggggtacctt tgactttgcc gagaaggaac
       61 accgtctcgc cgaggtcgaa ctgaccctcg ccgagcccga cgtctggaac gatcctgaag
      121 aagcgcagcg cctcaacaag gagcgctcgg cgctggagca ggtggtcaaa ggcctgcgcg
      181 atctgcacga gaccctcaat gacctgcggg atctggccga gcttgcgtct accgaagaag
      241 accacgatgc cctgacggag gtcgacgagg agctcgacga tctcgagcgg cagctcgaat
      301 ccctggagtt ccgacgcatg ttccagggcg agatggacga ggcgaacgcg tacgtcgaga
      361 ttcagtccgg atccggcggc actgaggccc aggactgggc ggaaatgctc cttcgcatgt
      421 atctgcgctg gggagaaaag cacggcttcg acaccgagat caccgagcta tcgcccggcg
      481 aggttgccgg catcaagggg gccacggtgc agttccaggg tgatcacgcg ttcggctggt
      541 tgcgaacgga aaccggggtg caccggctgg ttcgaaagtc gcctttcgac tccggtaatc
      601 ggcgtcacac ctcgtttgct tcggttttcg tcgcgcccga gatcgacgac gacatcgaga
      661 tcgatatcaa tcctgccgat gtgcgggtgg acacgtatcg ggccagcggg gccggtggcc
      721 agcacgtcaa ccggacggac tcggccgttc gcctgaccca cctgccaacg aacacggtcg
      781 tgcagtgcca gagcgagcgt tcgcagcacc agaacaagga caaggcattc aagcagctgc
      841 gcgcaaaact ctacgagctg gccgagctcg aacgcaacgc ggagcggcag gccgtcgagg
      901 actcgaaggc cgacatcggc tggggcagcc agatccgctc ctacgtgctc gaccagtccc
      961 gggtaaagga cctgcgcacc cacgtagagc gatcggatcc gggtaacgtg ctagacggcg
     1021 acctggatga attcatcgaa gcgagcctca aagcaggctt gtagggctta attctcatga
     1081 ccgacgaatc ggacatcatc agacatcggc gcgagaagct cgcgcgctgg cgcgaaaacg
     1141 gacgggccta cgacaatcag ttccgccgcg acgcccttgc gcaggagctg catgctcgct
     1201 gcgtcgacct ggacaaggac gcgctggccg atgccggcat ccaggtggcc gtcgccgggc
     1261 gtgtgatgct gcgacgggtg atgggaaagg cgagcttcat cactctcaag gatacgagcg
     1321 gcacgatcca gtgctacctg agaacggaag acgtcggccc ggaagcctac gaggaattcg
     1381 acgacctctg ggacatcggc gacatcgtgg gcgtgcgggg caccatgatg cgcacgaaca
     1441 agggcgagct gaccgtcgct gccgcgagca tcgagctgct caacaaaact ctgcggcccc
     1501 ttccggagaa gtaccacggg ctcaccgatc acgagatccg ttatcgccag cgttatctgg
     1561 atctcatcat gaacgacgat acccggcggg tcttcgagat ccgctccagg gtgatcggca
     1621 agatccgcga atttatgcag gagcggggtt ttctggaagt cgaaacgccg atgatgcagg
     1681 tgatacccgg cggcgcgaca gcgcggcctt tcgtcacgca tcacaatgcg ctcgatctcg
     1741 agctgtatct gcgtgtcgcc cccgagctct tcttgaagcg cctgaccgtg ggaggcatgg
     1801 agcgcgtctt cgagatcaac cgcaacttcc gcaacgaagg gttgtcgacg cggcacaacc
     1861 cggagttcac catgctggag ttctactggg cctacgccga tttcgaagac ctcatcgctc
     1921 tgaccgagga gttgctgcag cttctcgccc gggacgttct cgggacgggg acgctcacct
     1981 atcaggggac ggaaatcgat ctggcgcggc cggcggaacg ctacaccatg gtcgaagcca
     2041 tcgaacggca cacgggcatt gcctcggggc agcttaagga ccccgatgcc ctggccgcca
     2101 ttctcaaggg catgaacgtt cagcccgagg cggactgggg tatcggcaag atgcagctcg
     2161 aactcttcga ggccagggtg gaagaccgcc tggtccagcc cacgttcatc acccactacc
     2221 ccaccgaggt atcgccgctg tctcgccgta acgacgagga tcctgaggtg gccgaccgtt
     2281 tcgagctctt catcgccggg cgcgagatcg ccaacggatt ttccgagctc aatgacccgg
     2341 aggatcaggc cgagcgcttc cgcgctcagg cgctgcttcg aagcggcggc gacgtggagg
     2401 ccatgcattt cgacgacgat tacatcaccg cgctcgagta cggtctgccg cccacggccg
     2461 gcgaggggct gggcatcgat cggctggtga tgctgctcac ggactccccg accatccggg
     2521 acgtgctgct gtttccgctg atgcggccgg tgcaagatgg ttgaggccgc ccgccggagc
     2581 gaggagcgcg tccgcctgga gccttcctgg aaagcggtgc ttggcggtga gttcgacaag
     2641 ccctacatga acaagctgcg ggagttcctg ctccgggaga agcgggcggg caaggtgatc
     2701 tatccccggg gcgataacat cttcgccgcc ctggacctga cgcccttgga ggacgtcaaa
     2761 gtcgtgatca tcggtcagga tccctatcac ggccccgacc aggcacacgg cctgtgcttc
     2821 tcggtgcagc ccggcatccc gcaaccgcca tccctcgtca acatctttca ggagatcaac
     2881 gaggagctcg gcggtcccgc aaagcgtctg gatgacacca acggctgtct ggagccgtgg
     2941 gccaggcagg gcgtgctgct gctcaacgcg gtgctgacgg tggagcgtgg tcgcgccggc
     3001 tcgcatcagg gcatgggctg ggagatcttt accgacaagg tggtcgacgt tctcaaccgg
     3061 gagcgggacg ggctggtctt tctgctgtgg ggaagctacg cgcagaagaa aggagccatc
     3121 ctggacgtgc aacggcattg cgtcctgaag gcgccccacc cttcgccgct gtctgcccac
     3181 cgtggttttt tcggctgcgg gcattttgcc cgggccaacg agtaccttca gcaacggggc
     3241 gtggccggga tagactggtt cgacgttgcg tgatgcgact atctggaaga aaacaccgaa
     3301 ctgatgatcg actcaagaga ggaagctgat gattgaatcg ccgactgaaa agctgcttgt
     3361 cgagcagcga gggcacacgc tgctcgtcac catcaacacg ccgcccgcaa atacctggga
     3421 tgcggaaagt ctgcaggggc tcaagaacgt cgtcgagcag gccaacgcag aacgcagcgt
     3481 ttacgccatc gtcatcaccg gtcagggtga gaagttcttc tcggcggggg ccgatctgaa
     3541 gctgtttgca gacggggatc cggcagtggc ctcggagatg gccggccagt tcggctcggc
     3601 tttcgagacg ttgtccgcgt ttcgcggggt cagcatcgcc gcggtgaacg gcttcgccat
     3661 gggcggcggg ctggagtgcg cgctggcctg cgacatccgt gttgcggagg cgcaggcgaa
     3721 gctggcgttg cccgaggcca cggtgggtct gttgccgtgc gggggcggaa cccagaacct
     3781 ggcctggatg gtcggcgaag gatgggcgaa acgcatgatt ctctgcggcg agcgggtgga
     3841 tgcggcaacc gcgttgaaga tcggcctcgt cgaggaggtg gtggatgccg gccagtcgct
     3901 tggtcgcgcg ctcgagctgg cggagatggt gggccggcag agcccgcccg cggtggccgc
     3961 ctgcaaggat ctcattcagc aggcgcgcag cggcaacatc agcaaagcct actcgacgga
     4021 gcgggccgcc ttcgtgggtc tgttcgatac gcaggaccag aaagaagggg tcaacgcttt
     4081 cctcgagaag cgttcgcctg aatgggtcaa cgggtaacgg cgtcatggaa ccagtcaatt
     4141 cagccaacgc gccggtgcag ctctttgagc atcctacgga gagcggccac gttcttgcgg
     4201 aagcgaggct caatttcgag ggcaccctca actcgctcag cctggagatg atcgacatca
     4261 tgtccccggc gctcgcggat tggtccggcc gtgctgagat cgtcgccgtt ctgttcaccg
     4321 gggccggcga tcgcgctttc tgcgccggcg gggacattca ggcgctctac cacgccatgg
     4381 tgaaaaacca tgagcatggt gaggtggtgg acgattaccc gtttcagttc ttcgagcggg
     4441 aataccgctt cgaccatcag atccacactt ttcccaagcc ggtgatcgcc gtgggccacg
     4501 gggtcgtcat gggaggcggg ctcggtatcc tctctgccgc gcggtaccgt ctggtgacgg
     4561 agcggtcgcg gattgccatg cccgaggtga ccatcggtct gtttccagac gccgggcaga
     4621 cctggctgct gagaaacatg cccgagcact tcgccacgtt tctcggagtg accggctctc
     4681 acatgaacgg cgcggacgcc ctgatggtgg gtatcggcac tcacgggatc gagcaggcgt
     4741 ccagggaaag cgtgcgcgag cggctttgcg cggcctcctg gcaggcagac gcgcatgccg
     4801 cggtgcagga agccctggct gacctgccgg cgggcgggtt tcccgaagcg caggtcgaac
     4861 gcctgccgga ctcgctgtcc gtggcaggtg gcatcgctga cgtggccgcg tccattgccg
     4921 ccctgcgtgg cggcagcgac tggatcgacc gcggtatcgg cgccatgcag tcgggttgcc
     4981 cgaccacggt ggggatcgtc gtggagcagc tgcgccgagc cggggacatg tctttggcgg
     5041 acacgtttcg gatggagatg accgtggcca ctcattgtgc caacaaccac gattttgcgg
     5101 agggcgtccg cgccctgctg atcgagaagg acaacgagcc ggcatggcag ttcggcgatc
     5161 tcgattcgtt gccgcaggct tatgtggaca gccatttcgt cgagccgtgg gcgcagaacc
     5221 cgttggccga tctgaactag ggggaacaga cgatgagcag gataggattt gtcgggcttg
     5281 gcaacatggg cggccacatg gcgcgtaacc tggttgcggc cgggcaccag ctaaaagtgt
     5341 tcgacctcgt gccggatctc atggcggcgg tggagggcgc ccaagcgcag gagtccgctg
     5401 ccgcggcggc gacttcggtg gacatcttca tcagcatgct gcccgccggt cgccacgtcg
     5461 ccggtctgta cggcggtgag gacggcgtca tcgccgcggt ggacgaaggc acgctgttga
     5521 tcgactgcag caccatcgac ccgcagacgg cgagatcggt ggcggcggag gcgggcgctg
     5581 ccgggctcgc gatgatcgat gctccggtat ccggcggcac cgccggcgcg gaagccggga
     5641 cgctctcgtt catcgtcggg ggcgaggcag atgccctcga gcgcgcccgc gaggtgctca
     5701 atgtcatggg cagtaacata ttccacgccg gagccagcgg tgcaggccag atcgcgaaga
     5761 tctgcaacaa catgctgctg gccgtgctga tggcgggaac ggcggaggct ctggcgctcg
     5821 gcgtcagcaa cggtcttgat cctgccgttc tgtccgaggt gatgaaacgc tcctccggcg
     5881 gtaactgggc gctgaacgtc tacaatcctt accctggtgt catggagggc gcgcccgcgt
     5941 cccgcgacta cgagggcggt ttcctggtcg atctcatgac aaaggatctg gggctagcca
     6001 tggacaccgc ggagcagtcg cgcagcgccg tgcccatggg gtcgctcgcc aagaatctct
     6061 atcgcgtgca ccagcagctg cacgatgccg gcggtctgga cttctccagc attcagtggc
     6121 ttttcaaacc cgatctgaag gggtagccgg tcctgcgggg tacccggagg ccggtcagcc
     6181 ccgggcgctc agccgacact cgccgacgcg cagctcgtcg ggcgcggctt ctgcgatcac
     6241 ggcaatggcg atcccgccat gctcgctcag ccttgctgcc tcgacgaggg tgccgcaggc
     6301 gcggctggcg tcatcggcgt gggtcacctc cgttcctgcc gcaggcgctt cggggcctgt
     6361 ccagttcagt cgggcgagcc ggcgttttac cttgcccctg tgttgtgccc gggcgacgat
     6421 ttcctggccc aggtagcagc ctttgtcgaa gctgatcgcg cccagccggt cgaggtcgag
     6481 catctgcggc agaaacgact cgctggtcgg tgtgctgatg agcggccatc cttcagcgag
     6541 cctgtgggct tgccacacct cggtgggcgc atgtggcagg tcgtgccaga tcttctcagc
     6601 cttctcgagg tcatctgcca gcagcaggcc gatatcctca ctgatccgaa gcgccccggg
     6661 tggcggatcg ttcgcgaggt agccgaacac caggatgtct tcccccatat ccgccaggct
     6721 ggtgcgggag aacctcagat aggcttccag aaagctcgcg acgcgcggtg caagcgatcg
     6781 gtgcacgatg agatccaccg ggcctttctc ggcgtcatcg gcgcggacgc accagccgtt
     6841 gaagaccacc cgaccctgca ggttgcagac cgctgccggt tgcagacgct tcgggctgag
     6901 cttctgggtg tcgcaagtca cgtaaccctg gaggaacgtg gccgcatcgt tgccttcgaa
     6961 gcgcaggacg ctgaggtcgg gcaggtagca cacgcccgcc ggagcatccg gctcaccggt
     7021 taccgggtga gtttgcgaca gccacttttg tctgagcatg ctgatatggc gcctgagcgt
     7081 cagggtttcg atggtgcatg ttaaccgcat tggggttagt gtcgagcaat ggaaggcgat
     7141 gcgtctcagg gtcgaattta ctggcgcagc cggcgcggat tgctggagct cgagcttctg
     7201 ctgattccgt ttgtacgcga acgattcgag gaactgccgc aagacgatca ggaccgctac
     7261 gttcggcttc tcgagcatga ggatctcgac atctacgact ggcttcaggg gcgttcgaag
     7321 ccggaagatg aatccctgca cgccatcgtg gacagaatcc gcagacacaa ttcttctcga
     7381 gtttgaaacc gggcggcttc gccgcctcga ggtggccggc atcgcgactc tggctgccgc
     7441 cgccggctgg atgtggcttg gagcgtcggg cctggcgctg gtcgttctct ggttccgggc
     7501 ccggtggccg ttgcccgatg tcgtccgcta ccagctcgat gcgcggcgcg tcaggctcgt
     7561 aagcctgggg gtggtgcggg tgcgctggac cgagggctgg ttcaaggccg gggaagtgtt
     7621 ctccgacgag atcgacccgg cggacttcgc cgccttgcgc cggtttctca aagccgcggt
     7681 ccgagaccgg acggatcagc tgaagacggg ttgaagcacg tccagacggt ccatcgatcc
     7741 cgggaacagg atggtgtcgt ccgcccgact catcgtctcg ggatggtcga ggttgtagtg
     7801 caggcccctg gactcctttc ggaccagcgc cgagcgcacg atgagctcgg cgacatggat
     7861 cagatttctc aactcgatca ggtcgttgct gacgcgatag ttgctgtagt actcgtgcac
     7921 ctcctgcttg aggaggtcga tccgccgctg cgcgcgctgc aggcgcttgt ccgtcctgac
     7981 gatgccgacg tagtcccaca tgaagcgcct gagttcctcc cagttgtggg cgatgatgac
     8041 gtcctcgtcg gaatcaacca cctggctctc atcccactgc ggcacgttga cgtcccaccg
     8101 cggctctccg aggcggcgct tgatgtcccg ggcggcggct tccccgaaaa ccaggcattc
     8161 gagcagcgag ttgctcgcaa gccggtttgc tccgtgcagc ccggagcagc tcacctcgcc
     8221 cacggcgtat agcccgggca cgtcgctggc gccgttgcca tcgatgacca cgcccccgca
     8281 ggtgtagtga gcggccggca cgacggggat gggttcgttt gccatgtcga tgccgaagtc
     8341 ggcgcaacgc tgggcgatgg taggaaagtg gctctttatg aacgagcgcg gccggtgact
     8401 gatgtccagg tagacgcagt ccagcccaag ccgcttcatc tcgtggtcga tggctctggc
     8461 gacgatgtcg cgcggggcga gttccagccg gggatcgaaa cgctccatga atctctcccc
     8521 ggagggcagg cacagcacgc cgccttcccc acggaccgcc tcggagatga ggaacgattt
     8581 ggcgtggggg tgaaacaggc aggtggggtg gaactggttg aactccatgt tcgccacgcg
     8641 gcacccggcc cgccacgcca tggcgatgcc gtcgcccgtg gagctgtccg ggttgctcgt
     8701 atacagatac accttgctcg cgccgccggt cgccagaatc acgaagtggg cctggaaaac
     8761 ctccacgtgg ttggtggttc ggttgagcac gtaggcgccg gcgcagcggt ttgggtggcg
     8821 cccgaacttg ctcaggttga tcaggtcgat ggcgacccga tccgtgaaga gctcgatgtt
     8881 cgggtggtcg aggaccttgt cggtgagggt ttgggtgagc gctttgccgg tggcgtcggc
     8941 gacatgaaga atgcggcgat gagagtggcc accttcgcgg gtcaggtggt actcgttctc
     9001 gctgcgatcg aactcgatgc cccactgcac cagctggctg attacctggg gcgcgcgctc
     9061 cactgtctgc gccacgacgc tgtgatcgca cagaccggcg ccggcgttca gcgtgtcctg
     9121 tatgtgggcc tcgtaggtgt cgtccggatc cgtcaccgcg gctatgccgc cctgagccca
     9181 gtgagtggaa gcgctctcga cgtcaccctt gcacagaacg gtaacacgag catgctcggc
     9241 gaggtgcaga gcggccgaca ggccggccgc gccgccgccg atgacgagaa tgtctgtgga
     9301 gaaccgctgg cccatgtcca ccgcccggaa tcagttagcc aactagtata aacgggacgc
     9361 aatcggcaga atatcgggcc tcttggggaa caccggggtc gagccgggtc taagtggcaa
     9421 aagataccga caaggaactg gtaaggagag tccagaaggg tgaccgctac gcgttcgatc
     9481 ttttgttcag ccgctatcag cacaagatcc tcaacctcgt ttcccgctac ctcaaggatc
     9541 cccaggatgt ggaggacgtt acacaggaag ctttcataaa ggcgtttcgg gccttgccgc
     9601 gatttcgagg cgagagcgcg ttctatacct ggctgtaccg catcgcgatc aacaccgcga
     9661 agaaccacct cgtcgcacgc gggcggcgcc cgcccggagt agacgtggac atggaagatg
     9721 cggaaatcat ggacggtgcc gacgccctgc gggtgagcga aagcccggaa gcggtgctgg
     9781 aacgggatga gctttccgcc gccatcgatg tggcgatatc tcaattgccc gacgacctgc
     9841 gcagcgcggt aactcttcgg gaattcgatg gtctaagcta cgagcagatc gctcacatca
     9901 tggattgccc ggtgggtacg gtacgttcaa ggatattcag ggccagagaa tccatcgatg
     9961 ctcacatcga gccactgttg ccgcgcaggc ggtgacggcg aggttgctgg gtcagcggtt
    10021 ggtggtcgat acggctgcgc ggggagagac cgcgcaggac agggtaagga tagggtaaag
    10081 aaggtgctcg ttgaagacga gagcagggga agcaggagat gtcggaaaaa ctgagagagt
    10141 cgctttcggc gaccatggac ggcgaggctg acgagttcga actgcgtcgc gtcctcgacg
    10201 agatgcagaa agatggtggg ctgcgtgagg tttggagccg ctaccacatc gcaggaatgt
    10261 tgatgcgggg cgaacgcgtg catttcagca gcgatatccg ggaaagggtc tggagccagt
    10321 tgcagctcgg taccgatgac gaagcggttg aatcggccgc gctgccggca gctcaggccg
    10381 ggcccgcgga tggcccgcgg tccagactgg gcagattggc cgggctcgca gtggcggcgt
    10441 cggtcgcctt tgcggcggtg atcggcgtga tcggtctgtg ggacgaagga gcgggcgagc
    10501 ccccggcgac tgcgggcacg gtgaccatcg aagggccccg ggtcagcaat cctgcgttta
    10561 ccagcgagat ctcgccccgc gatctgcagc gagccaacgc gtacatgatg catcactttc
    10621 agcatcaggg gatgaaccag cccggcgtct cctcgttcgt gaagatggtg acctacgagc
    10681 gtcgatgaga cctgcacgct tcattctgct ggtgttcggc gtgttggttt cggccatttc
    10741 cgtggccgac gaggatcccc acgcgtggct ggccgatatg aaccgtgcct tcagcgagct
    10801 cagctacgac ggcatattca cctatttcag tggcgatgat ctcgacaccc tccgggtggt
    10861 gcacatggtg gtcgacgagg ttcagcggga acggctcgtg cacctcaacg gagcgccgcg
    10921 ggagatcgtg cgtacgggtg aggaggtggc ctgcatcctg atgccgggtg acgagctgct
//
LOCUS       DBNHLM010000174         3851 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1102701, whole genome shotgun sequence.
ACCESSION   DBNHLM010000174 DBNHLM010000000
VERSION     DBNHLM010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1102701"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="AC16QF_07965"
     CDS             <1..940
                     /locus_tag="AC16QF_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448532.1"
                     /translation="VSMERRGQEPEAEETDLDREYERQALGMSVQALVTRILEDQHLK
                     LRTDILRSQRHFAKQVAAEIGKAQAESSAAGGAGEQPARSAWTFLRSAAVVALFAGAG
                     LLYHQTQTSIQTAVDRIDSAETLMSDTTRQISRELADEPEETEGDEVARGNLLAALQW
                     AMNLGSRHAFEEPAFSSRRVEELRDLLTNLATAGFSGTVRLESHLGEFCLVPDGAGGF
                     TLADPDLPVQACMLIGHPQEESTFVSERQSLDFVTFLQSTPLLRASGIRVELVAKDRD
                     QSARRYDFPSAVASAGEWNRIAELNNRVEYALITAE"
     gene            complement(963..2168)
                     /locus_tag="AC16QF_07970"
     CDS             complement(963..2168)
                     /locus_tag="AC16QF_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6448533.1"
                     /translation="MDFSFSEGDRRFQLEVREWLEEAWPEEMRARQARSALGKLTKDD
                     LVRWQKRLAERGWAATNWPVEHGGAGFTPTQNYIFDLERARVGAPGVVPFGITMVAPV
                     IMKFGTPEQKERYLPDILASNVWWCQGYSEPGAGSDLASLKTKAEDRGDHYLVNGVKT
                     WTTLAQYADMMFCLVRTSQEDIRQLGISFLLIDMSSPGISVNPIITLDTPAEGFQEIN
                     MVYFEDVKVPKENLVGEEGKGWTCAKYLLEFERGNAYSPGLKHSLAHVKAMAAGEAGA
                     GGIEVGDLEARIRDVEIQILAMEFTELRILGALAAGQNLGPESSMLKTRGTELQQAVT
                     ELALEVVGHYALPLDQSLPVEGDNFAPVGPEYANGVAQDYFNTRKVSIYAGSNEIQRN
                     IMSKLVLGL"
     gene            2420..3250
                     /locus_tag="AC16QF_07975"
     CDS             2420..3250
                     /locus_tag="AC16QF_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043711 - pilus organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis chaperone"
                     /protein_id="HIR6448534.1"
                     /translation="MTTCRWVWAVLLAAAAALLSPTALAGAVLFIHPTLVVFDEDTRS
                     ARITLTNRGDETGTFECGWMELTMNPEGALRRLEDQPGWSVAPHLRFSPRRVTLKPSQ
                     SQVIKIALRPDAKLPEGEYYSHFRILTLNSEAPRGPDIESRDEAPESSGVSVQARTAV
                     AIPIVWRNGDAVMAARIQSLSLNREEKQLTVEVEREGPLSSRGFVHVILAPPGEDAEP
                     FGDPAPLVIYPNLAGRSVTLDLPDLPDGNPTGELTVVYSTEGSSERKGRVLATRSMSI
                     "
     gene            3267..3761
                     /locus_tag="AC16QF_07980"
     CDS             3267..3761
                     /locus_tag="AC16QF_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448535.1"
                     /translation="MAPVRAAPALTACLLGCLLVQQTAIAGPAPRPIEILEVFSPSFG
                     TWLAGPSGRQLVLFTDDTVGGADAADYLFGARSGEFVISGSANQAINILVDNTFATGG
                     IVVNQVICQYHRDLPARCDGAGVDARSGNNRTLKIGIDITTSQAHTGGDAPTINMDVA
                     IIYL"
BASE COUNT          696 a         1284 c         1171 g          700 t
ORIGIN      
        1 ggtctcaatg gagcggcgcg gccaggaacc ggaggcggaa gagacggatc tggatcggga
       61 atacgagcgg caggcgctcg gcatgtccgt gcaggcactc gtgacgcgaa ttctggagga
      121 tcagcacctg aagctgcgca ccgacatcct gcggagccag cgtcacttcg ccaagcaggt
      181 cgcagcggag atcggcaaag cccaagcgga gtcctcagcg gccgggggtg ccggagagca
      241 acctgcccga tctgcgtgga cgttcctgcg ttcggcggcg gtggttgctc tgttcgccgg
      301 tgccgggctc ctgtatcacc agacccagac cagcattcag accgccgtgg accgtatcga
      361 ctccgccgag acgctcatga gcgacacgac ccgccagatc tcgcgcgagt tggccgatga
      421 gcctgaggaa accgaggggg acgaagtcgc ccgaggcaac cttctcgcag ctttgcagtg
      481 ggccatgaac ctcggctccc gccacgcctt cgaagagccg gccttcagct cacggcgtgt
      541 cgaagaactg cgcgacctgc tgaccaacct ggcaaccgcc ggcttcagcg gcaccgttcg
      601 cctggaatct catctcggag agttctgcct ggttcccgat ggagccggag gcttcaccct
      661 ggccgatccg gatctgcctg ttcaggcctg catgctcatc ggccacccgc aggaggaatc
      721 gaccttcgtc tccgaaaggc agtcgctcga cttcgtgacc ttccttcaga gcacgccgct
      781 gctgcgggcg tcgggcatca gggtggagct ggtggcgaag gaccgcgacc agtctgccag
      841 acgctacgac ttcccttcgg cggtagccag cgccggcgag tggaacagaa tcgcggagct
      901 gaacaatcgg gtggagtacg cgctgatcac cgctgagtga tcggacccct cacccggcgt
      961 gctcacagcc cgagcaccag cttgctcatg atgttgcgtt gaatctcgtt ggaccccgcg
     1021 tagatgctga ccttacgcgt attgaaatag tcctgcgcca cgccgttggc atactcggga
     1081 ccgacgggcg cgaagttgtc tccctcgacg ggcagcgact gatcgagggg cagcgcgtag
     1141 tggccgacaa cctccagcgc aagctcggtg accgcctgct gcagctccgt acctcgcgtc
     1201 ttcagcatcg aggactccgg gcccaggttc tgccccgccg ccagggcgcc gagaatgcgc
     1261 agctcggtga attccatcgc gaggatctgt atttcgacat cacgaatgcg cgcttccaga
     1321 tcaccgactt cgatcccgcc agcgccggct tctcccgcag ccatggcttt gacgtgggca
     1381 agcgagtgct tgagacccgg cgagtaagcg ttgccgcgct cgaactccag aagatacttc
     1441 gcgcacgtcc agcccttccc ttcctcgccc acgaggttct ccttcggaac cttgacgtcc
     1501 tcgaagtaga ccatgttgat ctcctggaac ccctcggccg gcgtgtccag agtgatgatg
     1561 gggttcacgg atatcccggg gctcgacatg tcgatcagca ggaagctgat cccgagctgc
     1621 ctgatgtctt cctggctggt gcgcaccagg cagaacatca tgtccgcgta ctgggccagg
     1681 gtggtccagg ttttgacgcc gttgaccaga tagtggtcgc ccctgtcctc ggctttcgtc
     1741 ttaagtgaag cgaggtccga gccggcacct ggctccgagt acccctggca ccaccagacg
     1801 ttggaagcga gtatgtccgg caggtagcgc tccttctgtt ccggcgtgcc gaacttcatg
     1861 atcaccggcg cgaccatggt gatcccgaag ggcaccactc ccggggctcc gacgcgcgcc
     1921 ctctccaggt cgaatatgta gttctgagtc ggcgtgaagc cagctccgcc atgctccacg
     1981 ggccagtttg tcgccgccca gcctcgttca gcgaggcgtt tctgccagcg aaccaggtcg
     2041 tccttcgtga gcttgcccag agcgcttctg gcctgacggg ccctcatctc ctcgggccag
     2101 gcctcctcga gccactctct gacctccagc tgaaaccggc gatctccctc ggagaatgaa
     2161 aaatccatat gaccctcttc tgctgcctga tgagctcgcc gcgcaaacgg ccggcgcatt
     2221 ttagcgcaac gtgaccctcg acgggtcgtg cgagcagata gcgtctaggt agcggagatc
     2281 ggcgataccc gtgggaaacc gaaatgcgca aggttcccag catccccaac gaagtacgcg
     2341 accaccggca ctcatggtat attgccttca tcagtcgtat ttccctgtgc taatgccgaa
     2401 gatcacctgc aaagccagcg tgacgacgtg ccgatgggtc tgggccgtgc tgctcgccgc
     2461 cgccgcagcg cttctttccc cgacggcact cgcaggcgcc gtgctgttca tccaccctac
     2521 cctggtcgtc tttgacgaag atacccgcag cgcccggatt accctgacca accgcggtga
     2581 cgagaccggc actttcgagt gcggctggat ggagctcacc atgaatccgg aaggcgcgct
     2641 tcgcaggctg gaggaccaac ccggatggtc ggttgctcca cacctgcgct tctcgccgcg
     2701 ccgggtaact ctgaaaccgt cgcagtccca ggtcatcaag attgccctgc gcccggatgc
     2761 gaagcttccc gagggcgagt actactcgca cttccggatt ctgaccctca acagcgaagc
     2821 cccacggggg ccggacatcg aaagcaggga cgaggcaccc gaaagctcag gcgtcagcgt
     2881 gcaggcccga acggccgtcg cgattccgat cgtatggcgc aacggggacg ctgtcatggc
     2941 cgcgcgtatc cagtccttga gcctgaaccg ggaagagaaa cagctcacgg tggaagtcga
     3001 acgcgagggt cctctttcct cccgcggctt tgttcatgtg atattggcgc cgcccgggga
     3061 agacgccgag cccttcggcg acccggcgcc tctcgtgatc tacccgaacc ttgcagggcg
     3121 ctcggtaacg ctggacctcc cggatctgcc ggacggaaac ccaacggggg agctgacggt
     3181 ggtctactcc accgagggca gttccgagcg caagggccgg gtcctggcta cccgttccat
     3241 gagcatctga gagcgcttgg gctggagtgg cacccgtccg cgccgcgccc gcactgaccg
     3301 cgtgcctgct gggctgcctg ctcgtgcagc agacggccat cgccgggccg gcgccgcgcc
     3361 cgatcgaaat actcgaggtt ttctcgccaa gcttcgggac ctggcttgca ggccccagcg
     3421 gtcgccagct cgtgctgttc acggacgata ccgttggcgg cgctgacgcg gccgactatc
     3481 tcttcggcgc aagatccgga gagttcgtga tttccggctc tgctaaccag gcgatcaaca
     3541 tcctcgtcga caatacgttc gctaccggcg gtatcgtcgt caatcaggtc atctgccagt
     3601 accatcgcga cttgccggca cgctgcgatg gggccggcgt cgacgcccga tccggaaaca
     3661 accgaacgct gaagatcggc atagacatca cgacgtccca ggcacacacc ggcggagacg
     3721 cgccgaccat caacatggac gtcgccatca tctatctctg atgggcgggc gcaccctcac
     3781 aggtggatgc cgtctacctg tgcaaggcca actctggcat cgagcaccac ggactcgatc
     3841 accgttctgc c
//
LOCUS       DBNHLM010000175         9284 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_863397, whole genome shotgun sequence.
ACCESSION   DBNHLM010000175 DBNHLM010000000
VERSION     DBNHLM010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9284
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_863397"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="AC16QF_07985"
     CDS             complement(<1..246)
                     /locus_tag="AC16QF_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448536.1"
                     /translation="MNETDRRAQIGLVAVIGLILWFGLNPFDFARINNVQWLDPGPGL
                     QFGDSGLAVSEQAFTWPSDTGPAAVTVEMWVRASRVSD"
     gene            complement(283..1200)
                     /gene="ccoP"
                     /locus_tag="AC16QF_07990"
     CDS             complement(283..1200)
                     /gene="ccoP"
                     /locus_tag="AC16QF_07990"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HIR6448537.1"
                     /translation="MADMPSDFWSGWIITLTVASLAGLVWLIFSVYFGKDPDSDHTSP
                     VWDETLSEGANPAPMWWFWMILAALVFSAIYLMLYPGLGSHAGIMAWSQGGALEKSMN
                     RFDAAFAERRQGVVSAELADLHDDVDAMGTARGIFTRNCAACHGPQGLGQANAFPNLR
                     DTAWQWGGTPEAISQSITAGRRGAMVGWLPVLGEDGVEQLVTYVKTLSAGTSDPNAPA
                     ATQFTTYCSACHAADGTGVAMLGGPDLTDDVWLYGGSDDALRTSIAEGRNGIMPAFGD
                     RLDDVQLKLLVAWLTRPPKASARTEGAAR"
     gene            complement(1193..1336)
                     /locus_tag="AC16QF_07995"
     CDS             complement(1193..1336)
                     /locus_tag="AC16QF_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448538.1"
                     /translation="MSYFLFAGDMLVMAFMTILVVWVSLKSSGDQLERAARIPLEDER
                     HDG"
     gene            complement(1333..1956)
                     /gene="ccoO"
                     /locus_tag="AC16QF_08000"
     CDS             complement(1333..1956)
                     /gene="ccoO"
                     /locus_tag="AC16QF_08000"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020800672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HIR6448539.1"
                     /translation="MSGIGLHRKLEENSGLLIFGILFVSAIGGLVQVLPSLFQESLKT
                     PAANTRVYTPMELVGRDIYVREACSTCHSQQVRPLFAEVRRYGPYSRSGEFVYDRPFL
                     WGSKRTGPDLHRVGGKYSDAWHEIHMIDPRAVVPDSIMPAYPWLAERAANADGDIEAK
                     LRGLVTLGHPYTDEDIAGAAASLEGATELDALIAYLQMLGTGMEETP"
     gene            complement(1953..3392)
                     /gene="ccoN"
                     /locus_tag="AC16QF_08005"
     CDS             complement(1953..3392)
                     /gene="ccoN"
                     /locus_tag="AC16QF_08005"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045277 - respiratory chain complex IV
                     [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HIR6448540.1"
                     /translation="MSTTNDQTYSDRVVTTLVLLAIAWSIVGMTAGVWAAAQLVWPTL
                     DFGQSWLSYGKVRTLHTNAVIFGFGVTALMGTAFYSVQRTSHVRLFAPGLAWFTCWAW
                     QITVLIGGLSLLAGYNAGKEYAELEWPLDIAITVAWVCFAVVFFGTIARRRIKPIYVS
                     NWFYGGLILVIAMLHIVNSLALPVTLTKSYSLYAGAQDAIVQWWYGHNAVGFLLTGGF
                     LGMMYYFLPKQAEQPIWSYRLSIVAFWAFTYSYIWAGPHHLHYNSIPEWVQSLGMVMS
                     LVLLAPSWATMVNGIMTVSGAYEKLRTDPAMKFIVLSLAFYGLATFEGPMMSIKSINV
                     VSHFTEWTIGHVHSGALGWNALITFGTFYFLVPRLMGSKLYSVKLANWHFWLALAGTM
                     LYIVAMWGAGVSQGLLWLNIDDMGELRYSFTDIMAATVPYYVIRLLAGAVFLVGALLM
                     AYNLYRTVSGKRTIEVHAPSADDAAGASA"
     gene            complement(3396..4094)
                     /locus_tag="AC16QF_08010"
     CDS             complement(3396..4094)
                     /locus_tag="AC16QF_08010"
                     /inference="COORDINATES: protein motif:HMM:NF016764.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0016192 - vesicle-mediated transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HIR6448541.1"
                     /translation="MSGVHEPDTAVYTRRREELIDEGSGSLAVTTSIPASMMLRLLIR
                     PRSVFRLLADADPAPLVVFFKFALWLGLLPPLFAYIGASTFGWRVGVVEPLFLPQPTL
                     IAISIAYFCSLLFGFFSASIISRWMAVTYGARDSLGVHLAMMVIVGAPLAIASLMHLY
                     PNALLNAVVFVPTMIWSMYLLYRGLPTILHTTPEQGMLMASSIIGYLLVAAVSLLGLT
                     VFLWVMGIGPALGV"
     gene            complement(4140..5480)
                     /locus_tag="AC16QF_08015"
     CDS             complement(4140..5480)
                     /locus_tag="AC16QF_08015"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HIR6448542.1"
                     /translation="MTAAAAGCAQFGVPPLKRLYQSVPVPEDQPPLIVIPGAFGSKLR
                     RRATGEEIWPRSGFALLFSSYPDVEVTIDADTLDPIADEVEAFDIFRKGMGRDFYGAL
                     LKTLESIGGYQRAHPGTPPAPPARSYYVYPYDWRLDNVAAVEGLHRLIEQVVEDHGDP
                     NLRVDILGHSNGGLMARYYARFGTADMLTSRAAEPDWNGSHRIRRLLMVGTPNLGTMQ
                     PVLSHVRGEEIGLNDIPAEVVATCSGAPQLFPAPELTWLVDARGEDLNLDVYALETWR
                     ELGWSIFSDKARDRATSKHGVGRRGRAHLELLERYLAKHLERGRRFQHALARPARPDE
                     PEPFVFGGDCTPTVARLVLEKRGGVFVGREQPDRVAAPVRGVDYDGLINEPGDGVVTR
                     ASLTGQNATPVAPQLNVAHSVFLCEEHQLLTSNASFQDNLLYNLLTEFPQGAHG"
     gene            complement(5522..6613)
                     /locus_tag="AC16QF_08020"
     CDS             complement(5522..6613)
                     /locus_tag="AC16QF_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HIR6448543.1"
                     /translation="MAIHPLPPTAADIGPVWLTEVLHGSGGLSAEGRVKEVTWEKLGE
                     GAGMMSDLARLRMTYEGDAGSAPATLVVKQPSAVENNRLVAVKYNTYAREVRYFAELD
                     PVCAAQGPGIHLSEIDPDNNFVILMDDLSDYHIGDQIVGADLPQSEVCVDELAKLHGS
                     FWAAADDLDWLPHIHGSEHAGNMISGAQIGWDTMLKIFGEFVPDAINGERERFLDSIG
                     PLQHRLDTPPITVLHGDFRMDNLFFGQNPEQHPLIMLDWQGPLRGRGIYDVAQLLVQS
                     AQTEVRRSHERQLVERYVQKLTDQGVTGYGFDQAWEDYRVATLYLWAYVVVVSGTLEV
                     GSERAFAWMSKMVQRNASAIEDLDCLSLL"
     gene            6795..7841
                     /locus_tag="AC16QF_08025"
     CDS             6795..7841
                     /locus_tag="AC16QF_08025"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448544.1"
                     /translation="MDQEHESLMSHGRFDNPVIAALAGFKDRFREQPLASRLTDADRF
                     DGKRVLITGANSGLGYALAVDVARRGGSVTMACRSEIPEAGQRAIGESGSQNIEMRHC
                     DLSRMETLHGFCRSAAADGLRFDVVILNAATTLPRARPAPCGQDEMFVVNYLANFALV
                     HLLIDHGLLVPDARVQPRIVVISSDSHQGSSAIDYEEFGRYFDYGVRKAIANYSYFKL
                     LLNTFATELSRRLNSGDGIRVQVNVICPGPVNSNIIKAAPWALRLVLRAIFRVIFKSP
                     AEAARPVIYMAASSDFAGRTNDYLHMFSEKPMDSKVYDESAGSRLWRESAELWARIDD
                     KAADALSAIDKPGL"
     gene            7882..8427
                     /locus_tag="AC16QF_08030"
     CDS             7882..8427
                     /locus_tag="AC16QF_08030"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR6448545.1"
                     /translation="MRLAETLWFVAFVILSVAVPGLAAGSELEVGDPAPGFTLEGSDG
                     RTYSLSDYVGRQAVVIAWFPKAYTRGCTIECKSLAENGHLIRRFDVSYFMASVDPIED
                     NIGFAEQQKADFPLLSDPTKETARAYGVLSFAGFAKRHTIYIAKDGTIAAIDTDVRPA
                     TSAEDIATRLGELGVAAAPES"
     gene            complement(8434..>9284)
                     /locus_tag="AC16QF_08035"
     CDS             complement(8434..>9284)
                     /locus_tag="AC16QF_08035"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HIR6448546.1"
                     /translation="GGIEADVTVTRLGPETFWVVSAAPCQRRDLAWLAEHSAGFQADV
                     KDVTDQHAVIGVMGPDSRRLLTSLADADFGHDAFPFATSQPIAIANTALRATRITYVG
                     ALGWELYVPRAEAVTVYDAIAGAGPVHAGYHAMDSLRLEKAYRHWGHDITDEDTPIEA
                     GLSFTAAWDKPEGFIGREALLEQRADGVTKRLALFVLNDPDPLLYHDEPIWWNGRPVG
                     RITSGAYGHTLGRSMGFGYVAADAPFPRQALREGRFQIEIEDTLVDAACHLRAPFDPD
                     NREILR"
BASE COUNT         1684 a         2950 c         3067 g         1583 t
ORIGIN      
        1 gtccgaaacg cggcttgcgc gcacccacat ctcaacggtc accgccgcgg gaccggtatc
       61 cgacggccac gtgaaggctt gctcggagac agcgagtccc gagtccccga attgcaggcc
      121 gggccccgga tcgagccact ggacattgtt gattcgagcg aagtcgaagg gattcaggcc
      181 gaaccagaga atcagcccga tgaccgcaac gagcccgatc tgcgctcgac ggtctgtctc
      241 attcatgctc gggtgattcc ttggagcatc tggcgattcg gatcagcggg cggcgccttc
      301 cgtgcgtgcc gaggccttag gcggtcgggt caaccaggcg acgagcagct tcagctgaac
      361 gtcgtccagg cgatcgccga aggcgggcat gatgccgttg cggccctcgg cgatggacgt
      421 ccgcagcgcg tcgtccgagc cgccgtacag ccacacgtcg tcggtgaggt ccgggccgcc
      481 gagcatggcg acgccggttc cgtcggctgc gtgacaggcg ctgcagtagg tcgtgaactg
      541 cgttgccgcc ggcgcgttcg gatcgctcgt gccggctgaa agggttttga cgtaagtcac
      601 cagctgctcg acgccgtcct cgccgagcac gggtagccag ccgaccatgg cgccgcggcg
      661 gccggccgtg atggactggc tgatcgcctc gggcgtgccc ccccattgcc aggctgtgtc
      721 ccggaggttg ggaaaggcat tggcctggcc gagtccctgg ggaccgtggc aggccgcgca
      781 gttgcgggtg aagatgcccc gggccgtgcc catggcatcg acgtcgtcat gcagatccgc
      841 cagctcggcg ctcacgacgc cctggcgccg ctcggcgaag gccgcatcga agcggttcat
      901 gctcttctcc agagcgccac cctgcgacca tgccatgatc ccggcgtggc tgccgaggcc
      961 ggggtacagc atcaggtaaa tggcggaaaa gaccagggcg gcgagaatca tccagaacca
     1021 ccacatcggt gccgggttgg cgccctccga aagcgtttcg tcccataccg gcgaggtgtg
     1081 gtccgaatcg ggatccttgc cgaaatagac gctgaagatg agccagacga gccccgccag
     1141 gctcgccacg gtcagcgtga tgatccatcc gctccagaaa tccgagggca tgtcagccat
     1201 cgtgacgctc gtcctccagc ggtattcggg cggcgcgttc cagctgatcg ccggaactct
     1261 tcagcgatac ccagacaacg aggatggtca tgaacgccat gaccaacatg tcgccggcga
     1321 acagaaagta actcatggcg tctcctccat cccggtcccg agcatctgca ggtaggcgat
     1381 cagcgcgtcg agctcggtcg cgccctcgag gctcgccgcc gcgccggcga tgtcctcatc
     1441 ggtgtaaggg tgccccagcg tcaccaggcc gcgaagcttc gcctcgatgt cgccgtccgc
     1501 gttcgctgcc cgttccgcga gccatgggta ggcgggcatg atggagtcgg gcaccacggc
     1561 gcggggatcg atcatgtgga tctcgtgcca ggcgtcgctg tacttgccac cgacccggtg
     1621 aagatcgggc cccgtgcgct tggaacccca gagaaacggg cggtcgtaga cgaactcgcc
     1681 cgagcggctg tagggcccgt agcggcggac ctcggcaaag agcggccgca cctgctggga
     1741 gtggcaggtg ctgcaggctt cgcgcacgta aatgtcccgc ccgaccagct ccatgggcgt
     1801 gtagacgcgg gtatttgccg cgggcgtttt gagggattcc tgaaaaaggc tcggcagtac
     1861 ctgaaccagc ccgccgatcg cagacacgaa cagaatcccg aaaatgagca gacccgagtt
     1921 ctcctccagc ttgcgatgca gaccgatacc gctcatgcgg atgctcctgc ggcgtcgtcg
     1981 gccgagggcg cgtgcacctc gatcgtgcgc ttgccggaga cggtccggta aaggttgtag
     2041 gccatcagca gcgccccgac gaggaatacg gccccggcca gaaggcggat gacgtagtag
     2101 ggcacggtgg ccgccatgat gtcggtgaag ctgtagcgca gctcgcccat gtcatcgatg
     2161 ttcagccaca gcaggccctg ggagacgccg gcgccccaca tggcgacgat gtagagcatg
     2221 gtgcctgcca gcgccagcca gaagtgccag ttggccagct tgacgctgta gagcttgctg
     2281 cccatcaacc ggggcaccag aaagtagaag gtgccgaaag tgatcagggc gttccagccc
     2341 agagcgcccg agtggacgtg cccgatggtc cactccgtga agtgactgac cacgttgatg
     2401 ctcttgatgg acatcatcgg gccctcgaag gtggccaggc cgtagaacgc cagggagagc
     2461 acgatgaact tcatggcggg atcggtgcgc agcttctcgt aggcgccgct gacggtcatg
     2521 atgccgttga ccatggtcgc ccaggacggg gccagcagca ccaggctcat caccatgccc
     2581 agggactgca cccactcggg gatggagttg tagtgaaggt gatgagggcc cgcccatatg
     2641 tacgaatacg tgaacgccca gaaggccacg atggacagcc ggtagctcca gatcggctgc
     2701 tctgcctgct tgggcaggaa gtaatacatc atgccgagga acccgccggt cagcaggaaa
     2761 cccacggcgt tgtggccgta ccaccactgc acgatggcat cctgggcgcc ggcgtagaga
     2821 gagtaggact tggtcagggt gaccggcagg gccaggctgt tcacgatgtg cagcatggcg
     2881 atgacgagga tcaggccgcc gtaaaaccag ttcgagacgt aaatgggctt gatcctgcga
     2941 cgcgcgatgg tgccgaagaa gaccaccgcg aagcacaccc acgctacggt gatcgcgatg
     3001 tccaaaggcc actccagctc ggcgtactcc ttaccggcgt tgtagcccgc cagcagcgac
     3061 aggccgccga tcaacacggt gatctgccac gcccagcagg tgaaccacgc cagacccggt
     3121 gcaaagagac gaacgtggga cgtgcgctga acactgtaga acgcagtgcc catcagcgcc
     3181 gtcacgccga aaccgaagat gacggcattg gtgtgcaggg tgcgcacctt gccgtacgac
     3241 aaccagctct gtccgaagtc cagtgtcggc cacacgagct gcgcggccgc ccatacgccg
     3301 gcggtcatcc cgacgatcga ccaagcgatg gcgagcagaa cgagcgtggt gacgacccgg
     3361 tcgctgtagg tctgatcgtt cgtggtgctc aaatatcaga ctccaagggc gggaccgatg
     3421 cccatcaccc acaagaacac ggtgagcccg agcaggctga cggcggcaac cagcaggtag
     3481 ccgatgatcg atgaggccat gagcatcccc tgctcggggg tggtgtgcag gatcgtcggc
     3541 agtcctctgt agagcaggta catgctccag atcatcgtcg ggacgaacac gacggcgttc
     3601 agcagggcgt tgggatacag atgcatcagg ctggcgatgg ccagcggtgc gccgacgatg
     3661 accatcatgg ccagatgcac gcccaggctg tcccgcgcgc cgtaggtcac cgccatccag
     3721 cgactgatga tcgacgcgct gaagaagccg aagagcagcg agcagaaata ggcgatgctg
     3781 atcgcgatca gcgtcggctg cggcagaaac agcggctcca ccacgccgac ccgccagccg
     3841 aacgtggacg cgccgatgta agcgaagagt ggcggaagca ggccgagcca gagtgcgaac
     3901 ttgaagaaca cgaccagcgg cgcgggatcc gcatctgcga gcaggcggaa aaccgatctg
     3961 ggtcggatca gcaggcgcag catcatggag gccggaatgg aggttgtgac ggccagtgac
     4021 ccgctcccct cgtcgatcaa ctcttcgcga cggcgagtat agacggccgt gtcgggttca
     4081 tgaactccgg acaatccacg attgccggga tgcggccagg acgtggcgac gtcccggaat
     4141 cacccgtgtg cgccctgggg gaactcggtg aggagattgt agagcaggtt gtcctgaaag
     4201 cttgcgttgc tggtgagaag ctggtgctcc tcgcacagaa acaccgagtg cgcgacgttc
     4261 agctgggggg cgacgggcgt tgcgttctgg cccgtcagcg aggcgcgcgt caccacgccg
     4321 tcgccgggtt cgttgatgag accgtcgtag tccacaccgc gcacgggagc cgccacccgg
     4381 tccggttgct cgcgtccgac gaatacgccg ccgcgctttt ccagcaccag ccgcgccacc
     4441 gttggcgtgc agtcgccgcc gaagacgaat ggttccggct cgtcgggccg tgccggtcgt
     4501 gccagagcgt gctggaatcg ccttccgcgc tcgaggtgtt tggccaggta gcgctcgagc
     4561 agctcgagat gtgcccggcc ccggcggccc acgccgtgct tgctcgtcgc ccgatctctg
     4621 gctttgtcgc tgaaaatgga ccacccaagc tcgcgccagg tctccagcgc atagacgtcc
     4681 aggttcaggt cctcgccgcg ggcatccacc agccaggtca gctccggagc gggaaagagc
     4741 tgcggggcgc cggagcaggt ggcgaccacc tctgcgggaa tgtcattgag gccgatctcc
     4801 tcgcccctga cgtgggacaa cactggctgc atggtgccaa ggttcggggt gcccaccatc
     4861 agcagccgcc gaatcctgtg gcttccgttc cagtcgggct cggccgcgcg actggtgagc
     4921 atgtctgccg tgccgaagcg agcgtagtag cgagccatca gaccgccgtt cgagtggccg
     4981 aggatgtcga cgcgaagatt cgggtcgccg tggtcctcga ccacctgctc gatcagccgg
     5041 tggagtccct ctacggcagc aacgttgtcc agccgccagt cgtaggggta cacgtagtaa
     5101 gaccgcgcgg gcggcgccgg tggcgtaccc ggatgcgccc gctggtaacc gccgatcgat
     5161 tccaacgttt tcagcagcgc gccgtagaaa tccctgccca tccccttgcg gaagatgtcg
     5221 aaggcctcta cctcgtcggc gatcggatcc agggtgtccg catcgatggt gacctccacg
     5281 tcggggtagc tgctgaacag cagcgcgaag cccgagcgcg gccagatctc ctcgccggtg
     5341 gcccgccggc gcagcttgga gccgaaggca ccgggaatca cgatgagcgg cggctgatcc
     5401 tcgggcaccg gcaccgattg atagagccgc ttcagcggag gcacgccgaa ctgcgcgcag
     5461 ccggcggccg cggcggtcat cgccgccgac agaaaggcgc ggcgcggcca accatgtgga
     5521 actacaacag gctcaggcaa tccagatcct cgatggccga tgcgttgcgc tgaaccatct
     5581 tcgacatcca ggcgaaggcg cgttcgctgc caacctccag ggtgccgctg acgacgacca
     5641 cgtatgccca caggtaaagg gttgctaccc ggtagtcttc ccaggcctga tcgaagccgt
     5701 agccggtcac gccttggtcg gtcagtttct gcacgtagcg ttccaccagc tgtcgttcgt
     5761 gggaacggcg cacctcggtc tgagcgctct gcacaagcag ctgcgcgacg tcgtagatgc
     5821 cgcgccctcg cagcggaccc tgccagtcca gcatgatcag cgggtgctgc tcggggttct
     5881 ggccgaaaaa gaggttgtcc atccggaagt cgccgtgcag gacggtgatc ggcggcgtgt
     5941 ccaggcgatg ctgaagcgga ccgatgctat ccaggaaacg ctcgcgctca ccgttgatgg
     6001 cgtcgggcac gaattctccg aagatcttca gcatggtgtc ccagccaatc tgcgccccgg
     6061 agatcatgtt gccggcgtgc tcgctgccgt ggatgtgggg cagccaatcc aggtcgtcgg
     6121 ccgcggccca gaacgaccca tgcagcttgg ccagctcgtc aacgcacact tcggactgtg
     6181 gtagatccgc accaacgatc tgatcgccga tgtggtagtc ggaaagatcg tccatcagga
     6241 tcacgaagtt gttgtcggga tcgatctccg aaaggtgtat gccggggcct tgtgccgcgc
     6301 agacgggatc gagctcggcg aagtagcgga cttcccgggc gtaggtgttg tacttgaccg
     6361 cgacgagccg attgttctcg accgccgatg gctgtttgac caccagagtg gccggcgcgc
     6421 tgccggcatc gccttcatag gtcatcctca ggcgggcgag gtcgctcatc atcccggcgc
     6481 cttcaccgag cttttcccag gtgacttctt tgacgcggcc ttcggcggac agcccgccgc
     6541 tgccgtgcag aacctcggtg agccacaccg ggccgatgtc ggctgcggtt gggggcaatg
     6601 gatgtatcgc catcatcgtc ctcctggatg cacgggaaac ctggaaacga agaggataac
     6661 agggtgttcg gactaaacca ttccttggac gccgcggttg gtgacggccc gggccacgaa
     6721 tcagctttgc gccgtggttg gcaggacgta gtctttcacc cgcatacccc aagcccgggt
     6781 gcgggcgagg ggaaatggat caggagcacg agtcgctgat gtcgcacggc cgctttgaca
     6841 atccggtgat cgccgcgctg gcgggcttca aagatcgctt ccgcgaacag ccgctggcat
     6901 cccggctgac cgatgccgat cgcttcgacg gcaagcgggt tctgataacc ggcgcaaatt
     6961 cgggtctcgg gtatgcgctg gccgtcgacg tggcccggcg cggcggcagc gtgaccatgg
     7021 cgtgccgctc ggagattccc gaggcaggtc agcgcgcgat cggcgagagc ggatcgcaga
     7081 acatcgagat gcgtcactgc gacctgagca ggatggagac gctgcacggg ttctgccgtt
     7141 cggcggccgc ggacgggctt cgcttcgacg tggtcattct caatgcggcc accacccttc
     7201 cgagggcccg gccggcaccc tgcggccagg acgagatgtt tgtagtgaat tacctggcga
     7261 attttgcgct tgtgcatctg ctgatcgacc acgggctgct cgtcccggac gcccgcgtcc
     7321 agcctcgcat cgtggtcatc tcctccgaca gccaccaggg cagctcggcc atcgactacg
     7381 aagagttcgg acgctatttc gactacggtg ttcgcaaggc catcgccaac tacagctact
     7441 tcaagctgct gctcaacacc tttgccacgg agctcagccg ccggctcaac tcaggcgacg
     7501 gtatccgggt tcaggtcaac gtcatctgtc ccggtcccgt gaactcgaac atcatcaagg
     7561 cagcgccctg ggcgctgcgg ctggtcctgc gtgccatctt ccgtgtgatc ttcaagtcgc
     7621 cggcggaagc cgcgcgaccg gtcatctaca tggcggcaag ctccgacttc gcgggcagga
     7681 ccaacgacta tcttcacatg ttcagcgaga aacccatgga ttcgaaggta tacgacgaaa
     7741 gcgcgggttc ccggctgtgg cgcgagtccg ccgagctgtg ggccaggatt gatgacaaag
     7801 cagccgacgc attgtcagcc atcgacaagc ccggactgtg ataggctctg cactctctgg
     7861 tttgcgtgga ggctggccgt cgtgcgttta gccgagactc tctggttcgt tgcgttcgtc
     7921 attttatcag tcgccgtgcc cgggctggcc gccggcagcg agctcgaagt gggtgacccc
     7981 gcgcccggat tcacgctgga gggcagcgac ggtcggacct attcgttgtc cgactacgtc
     8041 ggccgacagg ccgtcgtaat cgcctggttc cccaaagcct atacccgcgg ctgcaccatc
     8101 gagtgcaagt cgctggccga gaacggtcac ctcatacgcc ggttcgacgt cagctacttc
     8161 atggccagcg ttgatcccat cgaggacaac atcggttttg ccgagcagca gaaggccgac
     8221 tttccgctct taagcgatcc caccaaggaa acggcccggg cctacggcgt cctcagcttc
     8281 gccgggttcg cgaagcgtca caccatttac atcgcgaagg acggcaccat cgccgccatc
     8341 gataccgacg tccgtccggc cacgtctgcc gaggacatcg cgacccggct gggtgagctt
     8401 ggggtggccg ccgcgcccga gagctagtcc cgctcagcgc agtatttccc ggttgtccgg
     8461 gtcgaagggc gccctgaggt ggcaggctgc gtccaccagc gtgtcctcga tctcgatctg
     8521 aaatcgtcct tccctcagcg cctggcgggg gaagggcgca tcggctgcga cgtagccgaa
     8581 acccatcgac cgtccgagcg tgtggccgta ggcgcctgat gtgatacggc ccacgggcct
     8641 gccgttccac cagatcggtt cgtcgtggta cagcaggggg tcggggtcgt tcagaacgaa
     8701 cagggcgagc cgcttcgtca cgccgtccgc ccgctgctcg agcagcgctt cgcggccgat
     8761 gaacccctcc ggcttgtccc aggcggcggt gaagctgagt cccgcctcga tgggcgtgtc
     8821 ctcgtcggtg atgtcgtgac cccagtgccg gtacgctttc tccagccgca gcgagtccat
     8881 ggcgtgatag ccggcgtgaa cggggcctgc cccggcgatg gcgtcgtaca ccgtgacggc
     8941 ttcggcccgg ggcacgtaga gctcccagcc cagggcgccg acgtaggtga tgcgcgtggc
     9001 gcgcagggcg gtgtttgcga tggcgattgg ctgtgaggtg gcgaagggaa aggcgtcgtg
     9061 gccgaagtcc gcgtccgcga gcgatgtcag cagtctgcgg ctgtcaggtc ccatgacgcc
     9121 gatgacggcg tgctggtcgg taacgtcttt cacgtcggcc tggaaaccgg cgctgtgctc
     9181 ggctagccat gccaggtccc ggcgctggca gggcgcagcg gatacgaccc agaacgtttc
     9241 aggaccaagc cgggtcacgg tcacgtccgc ttcgatcccg cccc
//
LOCUS       DBNHLM010000176        11375 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1341844, whole genome shotgun sequence.
ACCESSION   DBNHLM010000176 DBNHLM010000000
VERSION     DBNHLM010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11375
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1341844"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1041)
                     /locus_tag="AC16QF_08040"
     CDS             complement(148..1041)
                     /locus_tag="AC16QF_08040"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6448547.1"
                     /translation="MSPEEVLSRPPRVLTRDQRESYFEQGYAVFPGLVDDHWLQRLNA
                     VTDAFVEASREVVAGGDRRFDLEPDHCAAEPRIRRLNTPVELSETYWEFASRGPFADV
                     AEDLLGPSFKFHHSKLNFKWSGGGEEVKWHQDIQFWPHTNYDVLTLGVYLREVDAAMA
                     PMGVVPGSHAGPLYDLYDPSGRWTGNIRDEDLQSAGVENAEYLMGPAGSVTVHNCRSV
                     HGSAPNLSERPRPLLLCAYSAADAFPITTLTAGASHSEALIRGEPARWARFDPRPCLM
                     PPDWSRQGGYKSIFADQQKEA"
     gene            1187..2365
                     /locus_tag="AC16QF_08045"
     CDS             1187..2365
                     /locus_tag="AC16QF_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR6448548.1"
                     /translation="MSDIVYDFGSGRSDPGTFPTEALQRAAVTAIGKEAVTLTDYPGG
                     LGYPGLRRAMARRESEREGVDVNPDHLVITNGSMQAVTLTAEALQETAGDAIIVEEYS
                     YPGTLSAYRSLKFDMHGIPLSEQGMRLDRLEEKLVELDQAGRRPKFIYTITTYQNPTG
                     FVMPRANRLELIELARRYEIPVIEDNCYGDVHYEGPVEPALYALDDDPNQIYLCSLSK
                     ILAPGLRLGYLIARPPMLERILARRHDAGSNTLAAAIAAEFYRDGIWDHTRYANAALK
                     EKRDLLFDRLQAELDDVCVWSRPVGGLFVWVRIPDDINRKQLYELAQSRGFNYLPGIS
                     FHVETRNVPYLRLAFGHLTLDQIRDGIPVLAQCIRECRTSNEPRGFDSLFQDREGSSA
                     "
     gene            complement(2338..2730)
                     /locus_tag="AC16QF_08050"
     CDS             complement(2338..2730)
                     /locus_tag="AC16QF_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448549.1"
                     /translation="MYRALTEEVHAWWDAANSYSGDAANFYLEPRALGCFCEKLPGGG
                     SIAHMQVVYVEPGAVLRMLGGLGPLQGMGATGAVEFRLSAAADGARVEYRYHVTGFGA
                     ESMAEPVDGVQLGQFERLKRYAEEPSRS"
     gene            complement(2755..3141)
                     /locus_tag="AC16QF_08055"
     CDS             complement(2755..3141)
                     /locus_tag="AC16QF_08055"
                     /inference="COORDINATES: protein motif:HMM:NF024845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HIR6448550.1"
                     /translation="MECPKCPGTLETKTYGRKITVHRCSDCGGLWCKPEVLLEMKREW
                     MADVILDSGDPKLGQALDKLDNISCPECGVKMEKKTDDRQTHIWYESCPQCEGMFFDA
                     GEFTDLKYDTFMDRIRGALRGRRPSA"
     gene            complement(3153..3656)
                     /locus_tag="AC16QF_08060"
     CDS             complement(3153..3656)
                     /locus_tag="AC16QF_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6448551.1"
                     /translation="MPALSIAERDDFLAERGIVMRIGVVRADGSPLVTPIWFLHEDGA
                     IFFTPREKSEWFACLRRDPRVSLCIDEQPLPYRKVIVEGRAELVHDVGEDDVWRDLYR
                     RIAKRYVDDAEAEAYVQNTIDQPRGLYRVVMSEGEIKTWRMPVAGEAPEGIWHSRYYK
                     PDTKFNR"
     gene            complement(3677..4927)
                     /locus_tag="AC16QF_08065"
     CDS             complement(3677..4927)
                     /locus_tag="AC16QF_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6448552.1"
                     /translation="MNVKPVPTLDEEVNEIRLATAEIVNEDILPNEDRLWGWLADSAN
                     ATNEVKQEAKALRVDIQEKVKNARLWAPHLPREFGGMGLTFMQHAYMNEVLAYSPGAA
                     SLFGVVAPNSGNQKILVKYGTPDQHEKWLKPLTDGTAQSGFSMTEPHNPGSDPRSITT
                     RAELDGDEWVINGHKWFTSNGFAADFYIVMCRTSDPDDPGGANEKMTQIIVPRKTPGV
                     NIVRGVPVWGRASSHCEIVYDNVRVPKENQLGRTGTGHQAAQDRLGAGRVYHCMNSIG
                     SMWRAFDLMIDRLMTREVHGGEKLEDKQFMQGFVADSYIDIQTARLMTIHCAEKMESG
                     ADPRTDISAIKIFVPQAYHRVVDRAIQVWGAAGVSGDLPLASMYQGARTLRLADGPDE
                     VHRILIAKNILRRYHAGESWDFGN"
     gene            5083..6192
                     /locus_tag="AC16QF_08070"
     CDS             5083..6192
                     /locus_tag="AC16QF_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6448553.1"
                     /translation="MGTHELNYPHAEHPAPGTTVELAPGVRWLTMPMGGSLNHINLYL
                     LEDTTGWWIVDTGLSLDQTRELWHEIFSRELQGRPVLGVICTHFHPDHIGQSKMITDE
                     FRCSLYMTRAEYYQARAFSGSGPTYQSSWLGETFYQRAGMPADYLEQLREMWAHRSSE
                     GMSMPDMPAGYRRLEDGDVLTIGGHDWRIVVGSGHSPEHACLYCAALKIMISGDQILP
                     VITSNVSVHPTEPEANPLKDWMESHDRFLRVPDDTLVLPAHNLPFYGVRERLRQLIDH
                     HEDRMLAIEEHCVEPAVAKDLLPVLFARELDPRQMMMALGEAIAHLHLLVHRNRIERT
                     LHEDGCYRFLSIDPDLKRRAHPGDHDAPSERPVLV"
     gene            6218..6496
                     /locus_tag="AC16QF_08075"
     CDS             6218..6496
                     /locus_tag="AC16QF_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448554.1"
                     /translation="MQKGKLKRRIADYTYRMMHWGQNRLPPGIRSLVGVLLMVGGVFG
                     FLPVLGFWMLPLGVAFVALDVPGTRRHIDMWMLRLYRQAYLDEPTSNQ"
     gene            complement(6466..7332)
                     /locus_tag="AC16QF_08080"
     CDS             complement(6466..7332)
                     /locus_tag="AC16QF_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6448555.1"
                     /translation="MNHWMVGDVKITRVVEMEVAGGTRFILPDATRDACLPIEWLHPH
                     FMDEAGNLIMSIHALVVDTGSRRIIVDTCIGNDKQRSIPAWTNLQTSFLEDLGAAGYP
                     RETVDTVLCTHLHVDHVGWNTMLVDGEWVPTFPNARYLVAEKEFRYWDGEAEDEINQG
                     IMADSVRPIVAAGLMDFVDWEHQLCPEVALEPTPGHTPGHVSVHVRSNGKEALITGDC
                     IHHPCQMTRTDWCSSADYDRAAGEATRERLLESYVDSDVLIIGTHFATPTAGHVRRLD
                     GGGYWLDVGSSR"
     gene            7454..9274
                     /locus_tag="AC16QF_08085"
     CDS             7454..9274
                     /locus_tag="AC16QF_08085"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HIR6448556.1"
                     /translation="MGPIAKAVDVVRALSNVPTLLALKRGMKPRPVDEHDCFAANVER
                     NAAAFPDRTAIIFEGNSVTWAEFNAQCNRFASAFKAQGLRKGDAVSVMMENRIELLAT
                     IVALNKLGVVAGLINTNLRGRPLAHCITVTESRTCVFGEEVGEAVAEVKDELPLEEGS
                     GYLFVPDAGNGAAPNWAADLSRLAADADDADPPETGDVQLGDRALYIFTSGTTGLPKA
                     AVLSNRRFVATAALVHKAGLRCSEEDRIYICLPLYHGTGLLIGVGASFSSGASMFIRR
                     KFSASNFLREVREYQTSCLIYIGELCRYLMNTPAQPDDHDNPLVTMMGNGLRPDIWPA
                     FKSRFGIKRITEFYGASEGNVSFVNLLNKDCTIGMTGSKVALVRYDVDEDEIVRDENG
                     HCMEVAPGEPGLLLGHISEDAVFEGYTNAEATESKVVRNAFEDGDAWFNSGDLIRQVD
                     VGYSLGYPHYQFVDRVGDTFRWKSENVSTNEVGELINGFPDVKFCNVYGVEIPGADGR
                     AGMVAITLADGVTELDADALSRHVSDTLPPYARPVFVRVQPDIDVTGTFKMVKGDLRK
                     QGYDLDQITDPLFVMKPGESAYVPLDREFAGKIASGAAGY"
     gene            complement(9282..10346)
                     /locus_tag="AC16QF_08090"
     CDS             complement(9282..10346)
                     /locus_tag="AC16QF_08090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6448557.1"
                     /translation="MELWTLTHSRADNVLAVARRAEAAGWAGVMVVDSQNLAADSYVG
                     LAVAATGTQRIGLGTGVTNTVTRHPAVTASAAMAVQDVSGGRMVLGIGRGDSALAHLG
                     RSPARLPGFERYLDVLQRYLKGEAVPFEDIPMDDHIAAPVADLDLADHPSASRIRWFK
                     DDVAKVPVEVAATGPKVIEMAARLADRVMFTLGAVEERLSWAMETAMAARTGAGIPDD
                     PAFGAYVNLVCHRDRAVARDLVRGGLTTFARFSVMHGQIAGPADDHDRATLTSLHDGY
                     DMKAHTRADSSQAALLDDDFVDRFAIAGPPDYCVERLNRLASMGIEKVAISGPTAGTD
                     PVEARRALALLDEAVVPQLA"
     gene            10673..11293
                     /locus_tag="AC16QF_08095"
     CDS             10673..11293
                     /locus_tag="AC16QF_08095"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6448558.1"
                     /translation="MKRALLLDLGNVVLRVDFRRTFDYWAEAARVPAERLYRRWQLDE
                     AYELHEVGALAFEEYIAALSRRLMIDLPMEEWRAGWNAVFAGAFQDVQARLPTVRQSM
                     PVYLFTNTNATHQAAWSHLYADTLSHFDALYVSSEIGHRKPNVSAFRHVAEDMGYAPE
                     QIVFVDDTKENVLGARAAGMDARMVGSEADVVRILDELTGTEPGDS"
BASE COUNT         2058 a         3819 c         3522 g         1976 t
ORIGIN      
        1 ctcatgctgg cgagaatttc gctggaatcg gcaaagggcg agaccgcgag aaagacggcg
       61 atctccacgc ccatgagcac ggccggcagc acgagcacgt tcgtgcgcct ggcaaattga
      121 taggctcgct ccaacgacat cggcgcctca ggcctctttc tgctgatcgg caaagatgga
      181 cttgtaacct ccctgcctgg accagtcggg cggcatcagg cagggccggg gatcgaatcg
      241 cgcccagcgg gccggctccc ccctgatcag cgcttcgctg tgagaggcac ccgccgtcag
      301 cgtggtgatg ggaaacgcgt ccgccgccga gtaggcgcaa agcagcaggg ggcgcggccg
      361 ctcgctcaaa ttgggcgcag aaccgtgaac ggagcggcag ttgtgaaccg tgacgctacc
      421 cgccggcccc atcagatact ccgcgttctc cacgccggcg ctctgcaggt cctcgtcccg
      481 gatattgccc gtccaccggc cggaggggtc gtacagatcg taaagaggac ccgcgtggct
      541 gcccggcacc acccccatgg gcgccatggc cgcgtcgact tcccgaagat atacacccag
      601 ggtcaggacg tcgtagttgg tgtgtggcca gaactgaatg tcctgatgcc acttcacctc
      661 ctccccgccg ccgctccatt tgaagttgag cttggagtga tggaacttga agctgggtcc
      721 gagcagatcc tcggcaacat ccgcgaacgg gccgcggctg gcgaactccc agtaggtctc
      781 gctgagctcc acgggcgtgt tcaggcgccg tatccgcggc tcggccgcgc agtgatcggg
      841 ctccagatcg aaccgccggt cgccgcccgc gaccacctcc cggctcgcct cgacgaaagc
      901 gtcggtcacc gcgttcaacc gttgcagcca atggtcatct accaggcccg ggaacacggc
      961 atagccttgc tcgaaatagc tctcgcgctg gtcccgtgtc agcacgcggg gcggacggga
     1021 aagaacttcc tccgggctca tctcaagcac tccactcctg ttggccgtcc tgcctgccag
     1081 ggcttgcggc cgccgcgtgt tacgatacct caacggccgg ggcaattggc gcccgatcgt
     1141 tgccggcggc tagactcgca ggccggaccg gatcagggga cccatcatga gcgacatcgt
     1201 ctacgacttc ggcagtggcc ggtcggaccc cggcaccttt cccaccgaag cgctgcagcg
     1261 cgcggcggtt accgccatcg gcaaggaagc cgtcaccctc accgactacc cgggcgggct
     1321 tggctatccc gggctgcgcc gggccatggc gcgccgcgaa tccgagcgcg agggcgtgga
     1381 cgtgaatccg gatcacctgg tcatcaccaa cggatcgatg caggcggtca cgttgacggc
     1441 ggaggccctg caggaaactg cgggcgacgc catcatcgtc gaggagtaca gctaccccgg
     1501 caccctgtcc gcctatcgaa gcctgaaatt cgacatgcac ggcatcccgc tctcggagca
     1561 aggtatgcgg ctcgacaggc tcgaggagaa gctcgtcgag ctggatcagg cggggcggcg
     1621 ccccaaattc atctacacga tcacgaccta ccagaacccg accggcttcg tgatgcccag
     1681 agccaatcgc ctcgagctca tcgagctggc gagacgctac gagattccgg tcatcgagga
     1741 caactgctac ggcgacgtgc actacgaggg gccggtggaa ccggcgctgt atgccctgga
     1801 tgacgatccc aaccagatct acctgtgctc gctgtcaaag atcctggccc ccggactgcg
     1861 gctgggatac ctcattgccc gaccgccgat gctcgaacgc attctggcca ggcgtcatga
     1921 cgccggcagc aacacgctgg cggcagccat cgccgcggag ttctaccggg acgggatatg
     1981 ggaccacacc cgctatgcca acgccgcact caaggagaag cgcgatctgc tgttcgaccg
     2041 tctccaggca gagctggacg acgtctgcgt gtggtcgcga cccgtcggcg ggctcttcgt
     2101 gtgggtccgg atacccgacg acatcaaccg caagcagctc tacgagctcg ctcagagcag
     2161 agggttcaac tacctgcccg gcatcagctt tcacgtggag acgcgcaacg tgccctatct
     2221 gcggctagct ttcggccacc tgaccctgga tcagatccgc gacggcatcc ccgtcctggc
     2281 gcagtgcatc cgggagtgcc gcaccagcaa cgagccgcgc ggcttcgatt ccctgtttca
     2341 ggatcgggag ggctcttcgg cgtagcgctt caggcgttcg aactggccca gctggacccc
     2401 gtccacgggt tcggccatgg attcggcacc gaacccggtc acgtgatacc ggtactcgac
     2461 ccgggcgccg tcggcggccg cggacagacg gaactccacg gcgccggttg cacccatacc
     2521 ctggagcggg ccaagcccgc ccaacatccg cagaaccgcg ccaggctcga cgtagaccac
     2581 ctgcatgtgg gcaatgctgc cgccgccagg cagcttctcg cagaagcacc cgagcgcgcg
     2641 aggttcaaga taaaagttag ccgcgtcgcc ggagtaagaa ttggccgcgt cccaccaggc
     2701 atgcacctct tcggtgagag cgcgatacac gcgctcgggc ggcgcatcgc cggttcaggc
     2761 gctcgggcga cgcccgcgca gagcgccgcg aatccggtcc atgaaggtgt cgtatttcag
     2821 gtcggtgaac tcgccggcat cgaagaacat gccttcgcat tgcgggcagc tttcgtacca
     2881 gatgtgcgtc tgccggtcgt cggtcttctt ctccatcttc acgccgcact cggggcagct
     2941 gatgttgtcg agcttgtcca gcgcctgacc gagcttggga tcacccgagt cgaggatgac
     3001 gtccgccatc cactcgcgct tcatctcgag aagcacctca ggcttgcacc acaagccgcc
     3061 gcagtccgaa caccgatgca cagtgatttt gcggccgtag gtcttcgtct cgagcgttcc
     3121 gggacatttc ggacattcca tgaacagatc tcctaccgat tgaatttcgt atccggcttg
     3181 taataccgac tgtgccagat tccttccggt gcttctcctg ccaccggcat tctccaggtc
     3241 ttgatttcac cttcgctcat cacgacgcga tacagcccgc ggggctgatc gatcgtattc
     3301 tgcacgtaag cttccgcttc cgcatcgtct acgtaacgct tcgcgatccg ccggtaaaga
     3361 tctcgccaga catcgtcctc gccgacgtcg tgcacaagct ccgcgcgccc ctcgacgatg
     3421 actttgcgat acggcaacgg ctgctcgtcg atgcacagcg aaacccgcgg gtcgcgccgc
     3481 aggcaggcaa accattcgga tttctccctg ggagtgaaaa atatcgcccc gtcctcgtga
     3541 agaaaccaga tcggggtcac cagcggcgag ccgtcggcac gaaccacgcc gatcctcatg
     3601 acgatcccac gctcggcaag aaaatcgtcg cgctctgcaa tagacagtgc gggcacgccc
     3661 cctccccctt tagttcctag ttgccgaaat cccaggactc gccggcatgg tatcggcgca
     3721 gtatgttctt ggcgatgagg attcgatgga cctcgtccgg tccatccgcc aggcgcaggg
     3781 tgcgcgcgcc ctgatacatg ctcgccagcg gcagatcgcc cgacacgccc gcagcgcccc
     3841 agacctgaat cgcgcgatcc accacccggt ggtaagcctg aggcacgaat atcttgatgg
     3901 cggaaatgtc cgtgcgcgga tccgctccgc tttccatctt ttcagcgcaa tggatcgtca
     3961 tcagcctggc ggtctgaata tcgatgtagc tgtcggcgac gaagccctgc atgaactgtt
     4021 tgtcttccag tttctcgccg ccgtggactt cccgcgtcat gagacggtcg atcatcaggt
     4081 cgaacgcgcg ccacatggag ccgatggagt tcatgcagtg ataaacgcgg cccgctccga
     4141 gccgatcctg ggccgcctga tgcccggtac cggtgcggcc gagctgattc tccttcggca
     4201 cgcgaacgtt gtcgtagacg atctcgcagt gactggacgc tcggccccac accggtactc
     4261 cgcgcacgat gttcaccccg ggagttttgc gcggcacgat gatctgcgtc atcttctcgt
     4321 tggcgccgcc gggatcgtcc ggatccgacg tccggcacat cacgatatag aaatcggccg
     4381 caaacccgtt cgaggtgaac cacttgtgcc cattgatgac ccattcatcg ccatcgagct
     4441 ccgcccgggt cgtgatcgag cgtggatctg atcccgggtt gtgcggctcg gtcatggaaa
     4501 agcccgactg ggcggtgccg tccgtcagcg gcttgagcca tttctcgtgc tgatccggcg
     4561 tgccgtactt gaccaggatc ttctggttgc cggaattcgg cgccacgacc ccgaacaggg
     4621 aagccgcacc ggggctgtag gccagcacct cgttcatgta cgcgtgctgc atgaaagtca
     4681 ggcccatgcc gccgaattcc cgcggcaggt gcggcgccca gagccgggcg ttcttcactt
     4741 tctcctgaat gtcgacacgc agcgccttgg cttcttgctt gacctcgttg gtcgcgttgg
     4801 cgctgtcggc taaccaaccc cacaagcggt cctcgttggg cagaatgtcc tcattgacga
     4861 tctcggcggt ggccagccgg atctcgttga cctcctcgtc gagcgtcggt accggcttca
     4921 cattcatcga cacgacaatt cctccctcat gcggccagtc gattaatcta attgaacacg
     4981 gcccgcattg cgactgaccg gtacccgggt gccggggccc gctactgcag attgggcttt
     5041 ttggtatgct gctggatcga ggactgcaaa ccgggaacga cgatgggcac tcacgagctc
     5101 aactatccgc acgccgagca tccggcaccg ggaaccacgg tcgagctcgc cccgggcgtg
     5161 cgctggctga ccatgcccat gggcggctcg ctgaaccaca tcaatctgta tctgctggaa
     5221 gacacgaccg gctggtggat agtggacacc ggcttgagcc tcgaccagac ccgggagctg
     5281 tggcacgaga tcttctcccg agagctccag ggcaggcccg tcctgggcgt catctgcacc
     5341 cactttcatc cggaccacat cggccagtcg aagatgatca ccgacgagtt ccgctgcagc
     5401 ctctacatga cccgcgccga gtactaccag gcccgcgcct tttcgggcag cgggccgacc
     5461 tatcagtcca gctggctggg cgagacgttc taccagcgcg ccggcatgcc cgcagactac
     5521 ctcgagcaac tccgcgagat gtgggcgcac cggtcttcgg aaggcatgtc gatgcccgac
     5581 atgccggccg gctaccggcg cctggaggac ggggacgttc tcaccatcgg cggtcacgac
     5641 tggcgcatcg tggtgggctc gggccactcg cctgaacatg cctgcctgta ctgcgcggcc
     5701 ctgaagatca tgatctcggg cgaccagatc ctgcccgtga tcacgagcaa cgtcagcgtg
     5761 caccccaccg agccagaggc gaacccgctg aaggactgga tggaatccca cgaccgattc
     5821 ctgcgggtcc ccgacgacac cctcgtcctg ccggctcaca acctgccgtt ttacggcgtt
     5881 cgggagcggc tgcgccagct gatcgaccac cacgaggacc gcatgctggc catcgaggaa
     5941 cactgcgtcg agcccgcggt ggcgaaggac ctgcttcccg ttctattcgc acgcgagctc
     6001 gacccccgtc agatgatgat ggccctgggc gaagccatcg cccatctgca cctgctggtg
     6061 catcgcaacc gcatcgagcg gacgctgcac gaggacggct gctaccgctt cctgtccatc
     6121 gacccggacc tgaaacggcg agcgcatccc ggggaccacg atgccccgag cgagcggccc
     6181 gtgctggtct gagccggaag agcggagcca cacggcattg cagaagggca aactcaaacg
     6241 gcgaattgcc gactacacct accgcatgat gcattggggt cagaaccgcc tgcccccggg
     6301 gatccgctct ctggtgggcg tgctcttaat ggtcggcggc gttttcggtt ttctacccgt
     6361 gctcggcttc tggatgctgc cgctgggcgt cgccttcgtc gccctcgacg ttcccggcac
     6421 gcgacgccac atcgacatgt ggatgctgcg cctgtaccgc caggcctacc tggacgagcc
     6481 tacgtccaac cagtaacccc cgccatccag gcgccggacg tgaccggcgg tcggcgtcgc
     6541 aaagtgggtc ccgatgatca ggacgtcgga gtccacgtag gattccagga gccgctcccg
     6601 ggtcgcttca ccagcagcac ggtcgtagtc ggccgaggag caccagtcgg tccgcgtcat
     6661 ctggcaggga tggtggatgc agtcaccggt gatgagcgcc tccttgccgt tggatcgaac
     6721 gtgcacgctg acgtgcccgg gcgtatgacc gggcgtcggc tccagagcga cctcgggaca
     6781 cagctgatgc tcccagtcca cgaagtccat caggccggcg gccacgatgg gccgcacgga
     6841 atctgccatg atcccctggt tgatctcgtc ctcggcttcg ccgtcccagt agcggaactc
     6901 cttctcggcc accaggtagc gggcgttcgg gaacgtgggc acccactcac cgtccacgag
     6961 catggtgttc cagcccacgt gatccacgtg cagatgcgtg cacagcacgg tgtccacggt
     7021 ttcgcgcgga tagcccgcag cgccgagatc ttcgagaaag ctggtctgaa ggttggtcca
     7081 ggcaggtatt gaccgctgct tgtcgttgcc gatgcaggtg tcgacgatga tgcggcgcga
     7141 gcccgtatcc acgacgagcg cgtggatgct catgatcagg ttccccgctt cgtccatgaa
     7201 gtgcgggtga agccactcga tgggcaggca ggcatctctg gtcgcatccg gcagaatgaa
     7261 gcgggtgcca ccggccacct ccatttccac cacacgcgtg atcttcacat cacccaccat
     7321 ccaatggttc atcgccgctc cttgtctgcc cgggccgaac tgagcctggt tgagccccga
     7381 gcatgccaca ggtcgcttga tggtggcata cccgcaggat aaggtgcccc acgtagccga
     7441 acgaggagaa agcatgggcc cgatcgccaa ggccgtggac gtcgtccggg cgctgagtaa
     7501 cgtgcctacg ctgcttgctc tcaagcgcgg catgaagccg aggcccgtgg acgagcacga
     7561 ctgcttcgcc gcgaacgtgg agcgcaacgc cgccgcgttc ccggacagaa ccgccatcat
     7621 cttcgaaggc aactcggtaa cctgggccga gttcaacgct cagtgcaacc gctttgcgtc
     7681 ggcattcaag gctcagggcc tccgcaaagg cgacgcggtc agcgtgatga tggagaaccg
     7741 catcgaactg ctggcgacca tcgtggcgct caacaagctg ggcgtggtgg ccggcctgat
     7801 caacaccaac ctacgcggcc gacccctggc tcactgcatt acggtgacgg agtcgcggac
     7861 gtgcgtgttt ggagaagagg tgggcgaagc cgtcgctgaa gtgaaggacg agttgcccct
     7921 ggaagagggc tccggctacc tgttcgttcc ggatgcaggt aacggcgcgg caccaaactg
     7981 ggcggccgat ctcagccgcc ttgcggccga cgccgacgac gccgaccccc cggagacggg
     8041 cgacgtgcag cttggcgacc gggctctgta catctttacc tccggcacca ccggattgcc
     8101 aaaagcggcc gtactctcca atcgccggtt cgtcgccacc gccgcgctcg tccacaaagc
     8161 cgggctgcgc tgcagcgagg aggacaggat ctatatctgt cttcccctct accacggcac
     8221 ggggctgctg atcggcgtag gcgcttcgtt ctcgtccggc gcaagcatgt tcattcgtcg
     8281 aaagttctcg gccagcaact ttctgcgcga ggtccgcgag tatcagacca gctgcctgat
     8341 ctacatcggc gagctgtgtc gctatctaat gaacacgccc gcccagcccg acgatcacga
     8401 caacccgctg gtcaccatga tgggcaacgg cctgcggccc gacatctggc ccgcattcaa
     8461 atcccgcttc gggatcaagc gcatcaccga gttctacggc gccagcgagg gcaacgtctc
     8521 gttcgtgaac ctgctgaaca aggactgcac catcggcatg acgggatcca aggttgcgct
     8581 cgtccgctac gacgtggacg aggacgagat cgtccgggac gagaacggcc actgcatgga
     8641 ggtggcgccg ggcgagcccg ggctgctgct cggccacatc agtgaggacg ccgttttcga
     8701 gggctatacc aacgcggaag cgacggaaag caaggtcgtt cgcaacgcct tcgaggacgg
     8761 cgacgcctgg ttcaactcgg gcgatctcat ccgccaggta gacgtaggct acagcctggg
     8821 ctatccccac taccagttcg tggaccgcgt gggggacacc ttccgctgga agtcggagaa
     8881 cgtctccacc aacgaggtgg gcgagctgat caacggtttc cccgacgtga agttctgcaa
     8941 cgtttacggc gtcgagattc cgggggcgga cggccgtgcc ggcatggtcg ccatcaccct
     9001 ggcggacggc gtgacagaac tcgacgcgga cgccctgtcc cgtcacgtga gcgacacgct
     9061 gcccccctac gccaggcccg tcttcgtcag ggtgcagccg gacatcgacg tcaccggcac
     9121 attcaaaatg gtcaagggcg atctgcgcaa gcagggttac gacctggacc agatcactga
     9181 tccgctgttc gtcatgaagc cgggtgaaag tgcctacgtg cccctggacc gggaattcgc
     9241 cgggaagatc gcttcaggcg cagccggcta ctgaggcgcg ttcacgcgag ctgaggcaca
     9301 accgcttcat cgagcagcgc cagtgcccgg cgcgcctcaa ccggatcggt gccggcggtg
     9361 gggccgctga tcgcgacctt ctcgatgccc atcgacgcga gccggttcag gcgctcgacg
     9421 cagtaatcgg gagggcccgc gatggcgaaa cgatcgacga agtcgtcgtc cagcagagct
     9481 gcctggctcg agtccgcccg cgtgtgcgct ttcatgtcat agccgtcgtg caggctggtc
     9541 aacgttgccc ggtcatgatc atcggcggga ccggcaatct ggccgtgcat gacggaaaat
     9601 cgtgcgaagg tcgtgagccc gccccggacc aggtcccggg caaccgcgcg gtcacgatgg
     9661 caaaccagat tcacgtacgc gccgaatgcc ggatcatcgg gaatgccggc gcccgttctg
     9721 gccgccatgg ccgtttccat tgcccagctc agtcgctcct ccaccgcgcc aagggtgaac
     9781 atcacacggt cggcgagccg ggcagccatc tcgatcacct tgggccccgt ggccgcaact
     9841 tccaccggca ccttggcgac gtcatccttg aaccaccgga tgcggctggc gctcgggtga
     9901 tcggcaagat cgagatcggc aaccggagcg gcgatgtggt cgtccatggg aatgtcttcg
     9961 aacggcacgg cttcgccctt caggtagcgc tgcagcacgt ccagatagcg ttcgaacccg
    10021 ggcagtctag ccggcgatct gcccagatgc gccagcgcgg agtccccgcg cccgatgccc
    10081 agaaccatgc gcccgccgga gacgtcctga acggccatgg cggcagaggc ggtcacggcg
    10141 ggatgtcgcg tgaccgtgtt cgtgactccg gttcccaggc cgatccgctg tgtcccggtg
    10201 gctgccacgg ccaggcccac gtaggagtcg gccgccaggt tctgcgagtc caccaccatc
    10261 acgccggccc aaccggccgc ctctgcgcgg cgggcgacgg ccagcacgtt gtccgcacgg
    10321 gagtgagtca gggtccacag ttccattggc ggggcatcca tcgtcgatgg ttctccacca
    10381 tgatggttca acccctggaa aaagggaaac cccgggctgg cgcccggggc cgtccgccta
    10441 cagccagggg gaagggaggg agcatgggcc cgctgggggc atgccgtagc gaactgggca
    10501 ccggccgaat accatgcggg tcctgacgac tgcgcacctc cgtgccggac ctgccaggca
    10561 acgaccgaga ccttgaggca gaccttagag ccgattcgct gaacgccaac tgaatgcaga
    10621 aagacccgaa aatacggcgg cgcccaccac cggcgaccat ccgggccggg cgatgaagcg
    10681 cgccctgctg ctggatctgg gcaacgtcgt cctgcgcgtg gactttcggc gtaccttcga
    10741 ctactgggcc gaagcggccc gcgtgcccgc tgagcgcctt taccgccgct ggcagctgga
    10801 tgaggcatac gagctgcacg aggtgggagc cctcgccttc gaagagtaca tcgccgcgtt
    10861 gagccgtcgg ctcatgatcg acctgcccat ggaagagtgg cgcgccggct ggaacgccgt
    10921 cttcgccggc gcttttcagg acgtccaggc gcgtctgccc accgtgcggc agagcatgcc
    10981 cgtgtacctg ttcaccaaca ccaacgccac gcaccaggct gcctggagcc atctttatgc
    11041 agacaccctg agccacttcg acgcgctcta cgtctcgtcg gagatcggcc accgcaagcc
    11101 gaacgtctct gcgttccgcc acgtggccga ggacatgggc tacgcgcccg agcagatcgt
    11161 cttcgtagac gacaccaagg agaacgtgct cggcgcccgg gccgcgggca tggacgcgcg
    11221 catggtcggc agcgaagccg acgtcgtgcg cattctcgac gagctcaccg gcacggagcc
    11281 cggggattca tgagttgacg gcgtgcccga tctcggcgta gcggtcgagc agcggcaaca
    11341 ccgtatccgc gtccgccgag ggcaacccga atatc
//
LOCUS       DBNHLM010000177         8871 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1161900, whole genome shotgun sequence.
ACCESSION   DBNHLM010000177 DBNHLM010000000
VERSION     DBNHLM010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8871)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8871
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1161900"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..2092
                     /locus_tag="AC16QF_08100"
     CDS             14..2092
                     /locus_tag="AC16QF_08100"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HIR6448559.1"
                     /translation="MSGCSEGGPAETAGPVNTDTARIAAAQAEPDQWLSHGGTYAEQR
                     FSGLDEINDGNLDQLTLAWYFDFDTHRGQEGTPLMVDGVLYVTTAWSKVFALDAATGR
                     ELWRFDPKVPGSAGYNACCDVVNRGAAFYQGRVYVGTIDGRLIALDASDGRVVWSTVT
                     VPPDRPYTITGAPRAAKGKVFIGNGGAEYGVRGFVSAYDAETGELAWRFYTVPGDPEL
                     GPDGAASDEVLSSLAAPTWHGRAYLDYGGGGGTVWDAIVYDPELDQLYVGTGNGSPWV
                     RALRSEGRGDNLFLSSVLALDPDTGAYLWHYQETPGETWDYTSTQQITLATMEIGGEP
                     RRVLLHAPKNGFFYVIDRTTGKVVSAEKFVPVNWAQRVDLQTGRPVEAPGARFVDAPF
                     TAVVGGAGAHNWQPMSYSPGTGLVYIPAQEVPFVYTQEEGFAFKPGEWNLGVVLAGAR
                     LPADEATRREIVAANQGRLIAWDPVNQREAWRVEHGAPWNGGTLATAGNLVFQGLTDR
                     TFRAYRATDGKQLWSFDTQNVIITAPMSFAVNGEQHVAVMAGNGGGLPLTLPTFSGPL
                     QTPNGRLLVFKLGGTAELPPFEPEPLKPVIADVALTPDELDTGFDLFGRYCAACHGLE
                     TLSAGVLPDLRSSPALASSDAWRSIVHDGALETRGMIGFQARMSADQVEIIRGYVANE
                     ARKLESDADG"
     gene            2111..3475
                     /locus_tag="AC16QF_08105"
     CDS             2111..3475
                     /locus_tag="AC16QF_08105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014869595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR6448560.1"
                     /translation="MTPTERDLWLLPLGGCGEIGMNLNLYGHDGRWLMVDCGITFAEP
                     GESGPHIQMADPAFIASRRQRLCALIVTHAHEDHVGAVAHLWRQLQCPVYATPFTAEV
                     LRRKLAEARLLDDVPIHVVRSGDRLTLEPFDVEWVALTHSIPEPQALVIRTPAGSVFH
                     TGDWKLDPSPVIGADYDEARCRQLADEPIGTMVCDSTNAMVEGRSISEGELYAGLLDL
                     VSGARGRVIVGCFGSNVARLHTLIRVAADTGRFACLLGRSLHNYYAAARASGVWESDL
                     AFADAADIGYLPPDEVLVVATGSQGEPGAALDRLAADTHPLLNLEPRDTVLFSSRVIP
                     GNEPAVEKLIRRLERFGVTVIQDSELNMPIHASGHPARDELRDLYRWVRPDVALPVHG
                     EEAHMQANAELAREVGVPRQMTGRNGDLFMLAPVRGVRRDAARVGRLGLDNGRLVHVG
                     PPRR"
     gene            3483..4160
                     /locus_tag="AC16QF_08110"
     CDS             3483..4160
                     /locus_tag="AC16QF_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004574802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6448561.1"
                     /translation="MKIHADLSRRAVVKSPELPWVPSPLPGVDRRMLERDGGEIARAT
                     SIVRYAPESFFAQHEHSGGEEFFVLDGVFSDESGDYPAGWYVRNPIGSRHRPHTQSGT
                     TILVKLRQFQIGDDEYVRTDTNSGSWSPGEAEGVTIMALHAFGTERVSLARLATGTRM
                     PERAYPEGAEMFVLEGEIRDEHGVYPEGTWIRSPAGFSHRLRSPSGARLLVKTGHLPA
                     APASDVR"
     gene            4360..4749
                     /locus_tag="AC16QF_08115"
     CDS             4360..4749
                     /locus_tag="AC16QF_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6448562.1"
                     /translation="MTRFRTALALAVTLSAAAGCSERPADGPAAVRADDSPASLQSPP
                     AGQAAYAMACASCHDTGVAGAPRIGDDQSWSNRSPLWTAVLAEHAEDGYLGMPAMGGA
                     EELSERAVQDAVEYMLSATFPEAPPAD"
     gene            complement(4772..6280)
                     /locus_tag="AC16QF_08120"
     CDS             complement(4772..6280)
                     /locus_tag="AC16QF_08120"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF005565.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6448563.1"
                     /translation="MLSTPTEANIAAICESLGIEADAAEVAVYRDLVEGSIAGCALVD
                     EMPDDMADWTGGERTWERPAAADNPFNAWYVKTSVAGAPDGPLVGRTLAIKDNVLVGG
                     VPLMNGTSILEGYVPPVDATIVTRILEAGGTIAGKSVCEAYCFSGGSHTASSGFVTNP
                     HDASRAAGGSSSGSGALVAGGVVDMAIGCDQGGSIRMPASFCGVVGMKPTHGLVPYTG
                     ILGMGPAIDHVGPMTATVRDNALLLEVIAGPDGLDSRQYGAREQAYSAGLEDGARGLR
                     VGVLSEGFGREDSEADVDAKVRAAAERFGRLGADVSEVSVPLHAVASAICFSVIQSTV
                     ETLFGHDGALISRLDPAVASLVAAQHAWRDRADELPANVKVMLMSAEYLRREHGWEYV
                     AKGVNQIRRLRAAYDAALAEVDLLLLPTTPMKATELPAPDADPATVLGLAFAPLSNTM
                     AFNNTHHPAISVPCGMSDGLPVGMMLVGGHFEESLLYRAAFAFEQHEDWREL"
     gene            complement(6325..7089)
                     /locus_tag="AC16QF_08125"
     CDS             complement(6325..7089)
                     /locus_tag="AC16QF_08125"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6448564.1"
                     /translation="MDYLTVDEAKRATGLRLVLTQGVPGPWGESAKALFDLKGVAYHP
                     VAQKGGAANEELLAWTGHRNAPIAILDDEPPRASWLEILFLAERLGESPRLLPEDIDQ
                     RMLVIGLSSEICSPGGFAWQSRMFLLQGMAKAAGDQPENFPMLKDYSYSQEAADRAVA
                     SVKPILDRLARQLDMQAAAGSDYLVGGELTAVDVYWAFFSQLLVPLPEAICPMPGFLR
                     KSYGMLGTQLEEPAPASLLAHRDRILERHIALPLTF"
     gene            7271..8602
                     /locus_tag="AC16QF_08130"
     CDS             7271..8602
                     /locus_tag="AC16QF_08130"
                     /inference="COORDINATES: protein motif:HMM:NF015368.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003847 -
                     1-alkyl-2-acetylglycerophosphocholine esterase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6448565.1"
                     /translation="MRTRTPPYAAGSQTIFLHDESRPLDVCGGERRGVRTLITELWYP
                     VDTRQAASATRATYADYVFGDASVHRDLMTSTTFRHLTHETVAAGVTGQEIEAAIDEL
                     FSRERGSCLNAPLATHGGPFPVLIASPGDAGSRHNLESVCEHLASHGYAVIVPEHTGS
                     SPFAQLGRDPWLDETGPSAKQAEHVRLVREITDSRGAYGARAGFGQTYSPLRPHREQG
                     TAERQLNDALHERVRDLGTALNWLAAANARGPWAGRLRADRAGLLGRSFGAATALRAA
                     CADERLACTFAVAAPAWRNAPFLELRKPTFLLSGAEDDLLIGMASSQAGAKPTPENPH
                     PALFEAFIRSRAPAMWGLLANADHDAFTCAARYWWPGLKPGQARRALAPDETFELIEP
                     QRAQAVQQLTARAFFDCFLRMDATALARLDPATLAAAGLTFDTRNLEQVAR"
     gene            8599..8784
                     /locus_tag="AC16QF_08135"
     CDS             8599..8784
                     /locus_tag="AC16QF_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448566.1"
                     /translation="MTLAEITALISGGGHVEVELHAVDPMIYVAYLVSGNRRQPVTGR
                     ERVSLQAEALRSFQPRR"
BASE COUNT         1470 a         3096 c         2936 g         1369 t
ORIGIN      
        1 ctggctcggc ctgctgtccg gctgctcaga gggcggaccc gccgaaacgg cgggacccgt
       61 caacaccgac accgcccgca tcgcggcggc ccaggccgaa cccgaccagt ggctgtccca
      121 cggcggcacc tacgccgagc agcgattctc aggattggac gagatcaacg atggcaacct
      181 cgaccagctg acgctcgcct ggtacttcga tttcgatacc catcggggcc aggagggcac
      241 gcccctgatg gtggacggcg tgctgtacgt gacgacggcg tggagcaagg tgttcgctct
      301 ggacgccgct acgggtcggg agctctggcg cttcgacccg aaggtgcccg gaagcgccgg
      361 ttacaacgcc tgctgtgacg tcgtcaaccg gggcgcagcc ttctaccaag gcagggtata
      421 cgtcgggacg atcgacggtc gtctcattgc cctggacgcc agcgacgggc gagtggtctg
      481 gtcgacggtg acggtgccgc ccgacaggcc gtacaccatc accggcgctc cgcgagccgc
      541 caaagggaaa gtcttcatcg gcaatggtgg cgcggagtac ggcgtgcgcg gctttgtcag
      601 cgcctacgat gcggagacgg gcgagctcgc ctggcggttc tacacggtgc ccggcgaccc
      661 ggagctcggc ccggacggcg ccgcgtcaga cgaggtgttg agctccctcg ccgcacccac
      721 ctggcacggg cgcgcttacc tcgattacgg cggcggggga ggaacggtct gggatgccat
      781 cgtctacgat cccgagctcg accagctcta cgtgggcacg ggcaacggct ctccgtgggt
      841 gcgcgccctt cgctccgaag gtcgcggcga caacctcttc ctctcctcgg tgctggcgct
      901 ggaccccgac acgggcgcct acctctggca ctaccaggag acgcccggcg aaacctggga
      961 ctacacgtcg acgcagcaga tcaccctggc gaccatggag atcggcggcg agccccggcg
     1021 cgtgttgctc cacgcgccga agaacggctt tttctacgtg atcgatcgca caaccggcaa
     1081 ggtcgtctcc gcggaaaaat tcgttcccgt gaactgggcg caacgggtgg accttcagac
     1141 cggccggccg gtggaagcgc cgggcgcccg gttcgtcgac gcgcccttta ccgcagtggt
     1201 cggcggggcg ggcgcccaca actggcaacc catgtcttac agccccggca ccgggctcgt
     1261 ctacatcccg gcccaggagg tgcccttcgt ttacacccag gaagaaggct tcgcgttcaa
     1321 gcccggcgag tggaacctgg gcgtcgtgct cgcgggcgct cgcctgcccg cggacgaggc
     1381 aacgcggcgg gagatcgtgg cggccaacca gggccggctc atcgcctggg acccggtcaa
     1441 ccagcgggag gcctggcgag tcgagcacgg agccccgtgg aatggtggca cgctggccac
     1501 ggccggcaac ctggtctttc aggggctgac ggatcgcacg ttccgcgcat accgggctac
     1561 ggacgggaag cagctctggt cgttcgacac ccagaacgtg atcatcaccg cgcccatgag
     1621 ctttgccgtc aacggagagc agcacgtggc cgtgatggcc ggaaacgggg gagggctgcc
     1681 gctgacgctc cccacgttca gcggcccgct ccagaccccg aacgggcggc tgctggtatt
     1741 caagctggga ggcacggccg agctgccgcc cttcgagccc gagccgctca agcccgtcat
     1801 cgccgacgtg gcgctgacgc cggacgagct cgacaccggc ttcgacctct tcgggcgtta
     1861 ctgcgccgcc tgtcacggtc tcgagaccct gagcgcgggc gtcctgcccg accttcgctc
     1921 gtcgcccgct ctggcatcat ccgacgcctg gcgctcgatc gtccacgacg gcgcgctgga
     1981 gacgagaggc atgatcggct tccaggcgcg gatgagcgcc gatcaggtcg agatcatccg
     2041 gggctacgtg gccaacgagg cccgcaagct ggaaagcgat gccgacgggt agacgccggc
     2101 tcgacaagcc gtgacgccca ccgagcggga tctctggctc cttccgctgg gcggatgcgg
     2161 cgagatcggc atgaacctca atctgtacgg ccacgatggg cgctggctca tggtggattg
     2221 cggcatcacc ttcgccgagc ccggggagtc cggaccccac attcagatgg cggacccggc
     2281 tttcatcgcc tcgcgccggc aacgactctg cgcgttgatc gtcacccacg cccacgagga
     2341 tcacgtcggc gcggttgccc acctctggcg ccagcttcaa tgtccggtct acgcaacgcc
     2401 gttcactgcc gaggtgctgc gtcgcaagct cgccgaggcc cggcttctgg acgacgtgcc
     2461 cattcacgtg gtgagaagcg gcgaccgtct gaccctggag cccttcgacg tggagtgggt
     2521 ggctctgaca cattcgatcc cggagcccca ggcgctggtc atcagaaccc cggcgggtag
     2581 cgtgttccac accggcgact ggaagctgga tccctcgccc gtgatcgggg cggactacga
     2641 cgaggcgcgc tgccggcagc tggccgacga gccgatcggc accatggtct gcgactccac
     2701 caacgccatg gtggaagggc gttccatcag cgagggagag ctctacgccg ggctgctgga
     2761 tctggtcagc ggtgccagag gccgggtgat cgtcggctgc ttcggcagca acgtcgcccg
     2821 gctgcacacg ctgattcgcg tagccgccga taccggccgc ttcgcctgcc tgctcggcag
     2881 aagcctgcac aactactacg ccgccgcccg ggcgtccggc gtgtgggaat ccgatctggc
     2941 cttcgccgac gccgccgaca tcggctacct gccgccggac gaggtcctcg tcgtcgccac
     3001 cggcagccag ggcgagcccg gcgccgcgct ggaccggctg gccgctgaca cgcacccgct
     3061 gttgaatctg gaaccccggg acaccgtcct cttcagctcc cgggtcattc ccggcaacga
     3121 gccggccgtg gaaaagctca tccgccggct cgaacgtttt ggcgtcacgg tgattcagga
     3181 cagcgagctc aacatgccga tccacgcatc ggggcatcct gcccgggacg agctgcgcga
     3241 cctctaccgc tgggtgcggc cagacgttgc cctccccgtt cacggggagg aggcgcacat
     3301 gcaggcaaac gcggagctgg cgagggaagt cggcgtgccg cgccagatga ccgggcgtaa
     3361 cggcgacctc ttcatgctcg cgcccgttcg gggcgtgcgg cgggacgccg cccgggtggg
     3421 ccggctcggc ctggacaacg ggcgcctggt ccacgtgggc ccgccccgcc ggtgatggcg
     3481 ccatgaagat ccacgcggat ctgagccgac gggcggtggt aaaaagcccg gaactgcctt
     3541 gggtgccttc tccgctgcca ggggtggatc gacgcatgct cgagcgggac ggtggtgaga
     3601 tcgcccgggc gacgtccatc gtccgctacg cgccggaatc tttcttcgcc cagcacgagc
     3661 actcaggcgg ggaagagttt ttcgttctcg acggggtctt ttcggacgag tcgggcgact
     3721 acccggccgg gtggtacgtc cgcaacccca ttgggtccag acaccgtccc cacacgcaga
     3781 gcggcacgac tatcttggtg aagctgcggc agtttcagat tggcgacgac gagtacgttc
     3841 gcacagacac caactccggc agctggtcgc caggagaggc cgagggcgtc acgatcatgg
     3901 cgctgcacgc tttcggcacc gagcgcgtga gccttgcgcg gctggctaca ggtacccgga
     3961 tgccagagcg cgcctacccc gagggcgcgg agatgttcgt cctcgaaggc gagatccgcg
     4021 acgagcacgg cgtctatccg gagggcacgt ggatccgcag cccggcaggc ttctctcacc
     4081 ggctgaggtc gccctcgggc gcccggctac tcgtgaagac cgggcatctg ccggccgcgc
     4141 cggcgtcaga cgtcaggtaa gccgccttgc ccacctgctc caccaagacg tcccgagcgc
     4201 cttagagatc ggcgatgcct ttgccgtcct tgaagttgca ggcgtagaac tttctgacca
     4261 gtctggtttc tgccgcgcca ggaggctcgg tcaaacgccc gcttgcgcgc cggccgcatg
     4321 atacttagcc tttactcaac gaccagtcga gaaaccgccg tgaccagatt tcgcaccgcc
     4381 cttgctcttg ccgtcacgct gtccgcggcg gccggctgct ccgaacgtcc ggcagacggg
     4441 cccgccgccg tccgagccga cgactctccg gcgtcgctcc aatcgccgcc ggccggtcag
     4501 gccgcgtatg cgatggcatg tgcgagctgc cacgacacag gggtcgcggg cgcgccccgc
     4561 atcggggacg atcagagctg gtcaaaccgc tcaccgctct ggacggccgt gctggccgag
     4621 cacgccgagg acggctatct gggaatgccc gccatgggcg gtgccgagga actcagcgag
     4681 cgggcagtgc aggacgccgt cgagtacatg ctctcggcca cctttccaga ggcacccccg
     4741 gcagattgac ccctgcgcct gaccggggcg ctcaaagctc gcgccagtcc tcgtgctgct
     4801 cgaaggcgaa agcggcgcgg tagagcaggg actcctcgaa gtggccgccc accagcatca
     4861 tgcccacggg cagcccgtca ctcatgccgc agggcacgga aatcgccggg tgatgggtat
     4921 tgttgaacgc catggtgttg ctcagcgggg cgaatgccag cccgagcacg gtggccgggt
     4981 cggcgtccgg tgccggcagc tcggtggctt tcatgggcgt ggtcggcaac aacagcagat
     5041 ccacttcggc caacgccgca tcgtaggccg cccgcaaccg gcggatctga ttgacgcctt
     5101 tggccacgta ttcccagccg tgctcgcgac gcagatattc ggccgacatc aacatcacct
     5161 tgacgtttgc aggcagctcg tcggcccggt cgcgccaggc gtgctgagcg gccaccaggc
     5221 tggcaacggc aggatccagg cggcttatca gagcgccgtc atgaccgaat aaggtttcca
     5281 cggtggactg aatcaccgaa aagcagatgg ccgaagccac cgcgtggagc ggcacggaaa
     5341 cctcactgac gtccgccccc aggcggccga agcgctcggc ggccgcgcgc accttggcat
     5401 ccacgtccgc ttctgaatcc tcgcggccga acccctcgct cagcacgccc acgcgcaggc
     5461 cccgcgcacc gtcttccagc ccagccgagt atgcctgctc ccgcgctccg tattgccgac
     5521 tgtccaggcc gtccgggcca gcgatgacct cgagcagaag ggcgttgtca cgcacggtag
     5581 cggtcatggg cccgacatga tcgatggccg ggcccatgcc gaggatgccc gtgtacggca
     5641 ccagcccgtg ggtgggcttc atgcccacca caccgcagaa cgaagcgggc atgcggatcg
     5701 accccccctg gtcgcagccg atggccatgt ccacgacgcc gcctgcaacg agagcgccgc
     5761 tgcccgacga ggaaccgcca gcggctcggc tcgcatcgtg ggggttcgtg acgaatcccg
     5821 agctggcagt gtggctgccg ccggaaaagc agtaggcctc gcagaccgac ttgccggcaa
     5881 tggtcccacc cgcctcgaga atccgggtca cgatggtggc atcgaccggc gggacgtagc
     5941 cttcgaggat gctggtgccg ttcatcagcg gcacgccgcc cacgagcaca ttgtccttga
     6001 tggccagggt tcgcccgacc agcgggccgt cgggcgcgcc ggcgacgctg gtcttcacgt
     6061 accaggcgtt gaaagggttg tcggcagcgg ccgggcgctc ccacgtgcgt tcacccccgg
     6121 tccaatccgc catgtcgtcc ggcatctcgt ccaccagggc acaccccgcg atggaaccct
     6181 ccaccaggtc acggtaaacg gcgacctccg cggcatcggc ctcgatgcca agagattcac
     6241 agatggctgc gatgtttgct tcggttggcg tgctgagcat ggtcttctcc ctcccagagc
     6301 ggcgaagccg cgtgaaacca atcgtcaaaa agtcagcggc agcgcgatgt gccgctcgag
     6361 aatccggtcg cgatgggcca gcaggctggc cggcgccggc tcctcgagct gggttcccag
     6421 catcccgtaa gatttccgga gaaagcccgg catgggacag atggcttccg gcagcggcac
     6481 gagcagctgt gagaagaacg cccagtaaac gtccaccgcc gtcagctcgc ctcctaccag
     6541 gtagtcgctg ccggcagcgg cctgcatatc cagctgccgc gcaagccggt cgagtatggg
     6601 cttcacgctc gcgactgccc ggtcggccgc ttcctggctg tagctgtaat ccttgagcat
     6661 gggaaagttt tccggctgat cgccggccgc cttggccatc ccctggagca gaaacatgcg
     6721 ggactgccag gcaaagccgc cgggagagca gatctcgctg gaaagcccga tgacgagcat
     6781 gcgctggtcg atgtcctcgg gcagaagccg gggcgactcg ccgagccgct cggcgagaaa
     6841 gaggatctcg agccagctcg cccgcggcgg ctcgtcgtcg agaatggcga tgggcgcgtt
     6901 tcgatggccc gtccaggcca gcaactcctc gtttgccgcc ccgcccttct gcgccacggg
     6961 atggtaggca acgcccttca ggtcgaacag cgccttggcc gactcacccc aggggccagg
     7021 cacgccctgg gtgagcacga gccgcaaacc cgttgcccgt tttgcctcgt caaccgtcag
     7081 ataatccatc tcccctctac cccagcgatg tcgatcccaa gttatacccc gaaaacgtca
     7141 ggcatctcga ccgcggcgcg acccggtctg cgcgacccgg tctggagcga cccggtctgc
     7201 gcgacctgct ctggcgcccc tgtttcgacc ggggcatcat cggcagagaa gccgcaccag
     7261 caaactaacc atgcgcaccc ggacgccgcc ctacgcagcg ggctcccaga cgatattcct
     7321 ccacgacgaa tccaggcctc tcgatgtctg cgggggcgaa cggcgaggcg tcaggaccct
     7381 gatcaccgag ctgtggtacc cggtcgacac cagacaggcc gcaagcgcga ccagggccac
     7441 ctacgccgac tacgtgttcg gggatgccag cgtgcaccgc gacctcatga ccagcacgac
     7501 gttccggcac ctgactcacg aaaccgtcgc ggccggcgtg accggccagg agatcgaagc
     7561 cgcaatcgac gaactctttt cgcgcgagcg cggcagctgc ctgaatgccc ctttggcaac
     7621 acacggcgga ccctttcccg tgttgattgc atctcccgga gacgccggca gccggcacaa
     7681 cctggagagc gtctgcgagc atctggccag ccacgggtac gcggtgatcg tgcccgagca
     7741 caccggcagc agcccgtttg ctcagctcgg acgggatccc tggctggacg agaccggccc
     7801 ctctgcaaaa caggcagagc acgtccgcct ggtgagggag atcacggaca gccgcggagc
     7861 ctacggagca agggccggct tcgggcagac ctactcaccg ctgcgaccgc acagggagca
     7921 aggcacggca gagcgacagc tgaacgatgc gctccacgag cgggtgcgcg acctgggcac
     7981 cgccttgaac tggctggctg ccgccaacgc tcggggaccc tgggcaggac gcctgcgcgc
     8041 cgaccgcgcc ggtctcctcg gccgctcatt cggtgctgcc acggctctgc gcgccgcctg
     8101 cgccgatgag cgcctggcct gcacgttcgc cgtggcggcg cccgcctggc gcaacgcccc
     8161 gtttctcgaa ttgcgcaagc cgacgttcct gttgagcggc gccgaagacg atctgctgat
     8221 cggcatggcc agcagccagg ccggggcgaa gccgaccccg gagaatcctc accctgcgct
     8281 ctttgaagcc ttcatccgga gccgtgcgcc agccatgtgg ggtctgctcg cgaacgccga
     8341 ccacgacgcg ttcacctgcg cagcccggta ctggtggccg gggctgaagc cgggccaggc
     8401 gcgacgcgcc ctcgctccgg atgagacctt cgagctgatc gagccgcagc gggcccaggc
     8461 cgttcagcag ctcaccgcgc gggccttctt cgactgcttc ctgcggatgg acgcaacggc
     8521 gctcgccagg ctggatccgg cgacgctggc ggcggctggg ctgaccttcg acacccgcaa
     8581 cctcgagcag gttgcccgat gacgctcgcc gagatcaccg ccctcatcag cggcggcggc
     8641 cacgtcgagg tcgagctcca cgccgtcgac ccgatgattt acgtggctta ccttgtttca
     8701 ggcaaccggc ggcagcccgt cacggggcgc gagcgggtgt cgctgcaagc cgaggccttg
     8761 cgctcgttcc agccccggcg ttagggttcg ggctgagcgc ttctgggaga cagcaccgga
     8821 tgatcgacct ctccatggcg ggcaacgaag acgaggcgct cgcgctggcg c
//
LOCUS       DBNHLM010000178        13866 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1162006, whole genome shotgun sequence.
ACCESSION   DBNHLM010000178 DBNHLM010000000
VERSION     DBNHLM010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13866
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1162006"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="AC16QF_08140"
     CDS             <1..1040
                     /locus_tag="AC16QF_08140"
                     /inference="COORDINATES: protein motif:HMM:NF016174.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HIR6448567.1"
                     /translation="FVTEAYFGLIRNFRRFSWLLIYLFGSSPAICKSFVKNQPHELAS
                     FDEGSLYLPHATSLRMGRLGYQSDAQRSLHISYNGLEQYAASMHTALTRPYPPYEAIG
                     VKVDGEYRQLNASLLQIENEFYGTIRPKRRIVSGERPLNALRSRGVEYVEVRCLDLNP
                     FLHMGIDTVEIRFIDAFLLYCLLADSPEDSERESELMGKNQLAVVERGREPDLQLEQL
                     RGTITRKAWAEDVLHACRPLAELLDEATGSELYRHALSEQSSKNRQPALTPSARVLAV
                     MQTQGIPFFRFAMNQSIAHKGYFDEHPMRGEQLAEHQATSQQSLARQQEIEAADSVDF
                     DVFLKDYLALE"
     gene            1037..1678
                     /locus_tag="AC16QF_08145"
     CDS             1037..1678
                     /locus_tag="AC16QF_08145"
                     /EC_number="3.1.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF016239.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HIR6448568.1"
                     /translation="MNFLAHCLIAAQSTDPEEPDLVAGGFLGDFVKGPIGPELPPGLA
                     LGVRLHRRIDAFSNRHPAIQGSCNRFAGPLRRLAPVLVDIIADHCLAKHWEQFSNEPI
                     AAFTARTYDRIASHERWLPDHGHRFLSYMRDEDLLAGYARFEAMHRALCSVTRRLDRE
                     HLNADMLSATEANLPGLEADFLGYFPELVAHARQWLRDEGPPPGPQTPGTVQR"
     gene            1751..2728
                     /locus_tag="AC16QF_08150"
     CDS             1751..2728
                     /locus_tag="AC16QF_08150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HIR6448569.1"
                     /translation="MKAVLCKAFGPPEDLVMEEVANPEPGPGQILVDIHASALNFPDV
                     LMIAGKYQSLPPFPFSPGGEIAGTVAAVGDGATRFKVGDRVFGGTGHGGFAEQIALPE
                     MAMRPMPDEMSFVEASAISTTYGTSYYALKQRADLKPGETLLVTGAGGGVGLAAVELG
                     KAMGARVIAAASSAEKLAAARSAGADELIDYSDGELKEKVKALTDGKGADVIYEPVGG
                     DLFEQCMRCVNWYGRILVIGFAAGDIPRVPINLILLKSCQVVGVFYGAWSARCRDEAD
                     ANFKEILDLYRDGKIKPMIGRTYPLEEYAAALRCLSERQAIGKVVVTVR"
     gene            2725..3222
                     /locus_tag="AC16QF_08155"
     CDS             2725..3222
                     /locus_tag="AC16QF_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HIR6448570.1"
                     /translation="MNAWVFRDTSELWAVMLVASVGALLFAALIMEHVFGMAPCPLCL
                     MQRIWFFVAGAFAFASLLHNPRLGIYPLCSMVAAVIGGGFSIRQLYLQGLPPDQVPSC
                     GPDMSYMLEAFPLTDVLIAMTRGTGDCAEVAWSFLGISIPGWALVGFAALAVMAALQL
                     RAGTR"
     gene            3317..4507
                     /locus_tag="AC16QF_08160"
     CDS             3317..4507
                     /locus_tag="AC16QF_08160"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6448571.1"
                     /translation="MSRAQLINMARHNIGHAKAGTIEQAPDIVKVPVQNYFDPDRWQL
                     EMDRIFRRMPLVLAMTDELRNPGDYKAMEAVGMPVLLSRGEDGEVRAFVNMCRHRGSQ
                     VMLPGCGNAHRFTCPYHAWTYDQEGALVGVYAPKDFGDVDKGENGLVPLPVAERAGMI
                     WVIVNPQATLDIDTFLSGYDELLGHFHFEDWHLFEQRTIAGPNWKIAYDGYMDLYHLP
                     ILHKDTFGDQFPNQALYYAWGPHQRVSSPDPTLAGYEQLPESEWNEAHMLAGVWTIFP
                     HVSVAGFDGGGRSVMVSQLFPGKTPQESVTVQNYLMEKAPDAEQAEAAEQMFKLLEYV
                     VQEEDYATGLRQQKAIMTGAMSHVMFGRNEEGGQRFHRWLDTLLATDDEDLNALFAGG
                     ERYC"
     gene            4767..5519
                     /locus_tag="AC16QF_08165"
     CDS             4767..5519
                     /locus_tag="AC16QF_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HIR6448572.1"
                     /translation="MNANVRRILISNGKGGCGKTTIAANLAVSYANRGAKVALVDQDP
                     QASSSYWADQRSRELAPVHLVRAHQRANMYQTQTFHNRIPADISRVVVDGSTNPQDRE
                     LEMLLKQCDVILVPLLPSAIDIRAGSQFIKELITHRSFRTLPKPVGVIANRVQPNSTM
                     HARLEHFLKCLDVPWVATFRDSPLYPEAFERGEGVVDLIDCRAARREAPAWRALTAWI
                     ERQPVSRGCTARELRNRPIRAPRHVDDETLSA"
     gene            5621..7501
                     /locus_tag="AC16QF_08170"
     CDS             5621..7501
                     /locus_tag="AC16QF_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003161181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR6448573.1"
                     /translation="MLQLIDISLKRGSNQLFEHLTCTIHPGHKVGMVGRNGAGKSTLF
                     DMILGRLQPDEGEVSRPSGWVASHMAQEVNVTDRPAIEHVIDGHRELRAVERRIAQAE
                     SRRDDLTLAELHTRFGDVGGHQARARAGTILHGLGFAADEIEQPFRAFSGGMRIRLNL
                     AQALMAPADLLLLDEPTNHLDLETTVWLESWLQRFAGTLLVIAHDRAFLDGVCDHIVH
                     IEHGRANSYRGNYSSFERQRAEALAQQQAAHARQQAEIKHMQHFIDRFRAKASKAKQA
                     QSRMRALERMQAVAPVHADSPYRFSFANPEQMSHPLLSLEDVSVGYEGRAVIEHVRQT
                     LLPGARIGILGANGAGKSTLLKCLVGELEPLAGTLVRGRHSAVGYFAQHQLERLDGQT
                     SALATLTNERPQAREQWCRDYLGRWGFPADMVERPIVTLSGGEKARLVLALIALAQPA
                     ILVLDEPTNHLDLEMREALALALQDYAGALLLVSHDRSLLRRAVDEFWLVEGGRLASF
                     AGDIDAYAESRLRRSEPPSSERAPETNRKALRQAAAAQREAAKPLRAKLRHLEKEMDQ
                     IGVELREVESRLADGDVYRSLPPEELDDLLSAAGRLRKRLDATEQAWLSATEALEALE
                     QS"
     gene            complement(7540..8631)
                     /locus_tag="AC16QF_08175"
     CDS             complement(7540..8631)
                     /locus_tag="AC16QF_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR6448574.1"
                     /translation="MIKWLSSRKRQVLAGFTAMALLLSGYTFADELEDRLREAREQLD
                     AAAERLAELHRQMYEAEVSEGRYGKRAMIGILLTSMGDDDGLVIAGVTPDGGAKQAGL
                     AAGDRIVAIGGVRLDTGGADKPGKLLSNVVKDLEPGDPVKVEYVRDGETLKADVVTQP
                     KRAFVMKSLAMELDQLDPEFTAELIKEFEDLGELKELKELGKLEALHELGDLGELIGN
                     VVDVGTSLKLEDMDGDLARYFGVDAGVLVVAPPSQEGELKSGDVLLELDGKDVANARE
                     AMKTLAMSDGTIDAGVLRNGKKREVSIDCSQFSAGDHMAITIDPSIIRIRRGIDGDDG
                     VDVRIVVDEEVVVDEKGSKKAAADDSAGG"
     gene            complement(8641..9258)
                     /locus_tag="AC16QF_08180"
     CDS             complement(8641..9258)
                     /locus_tag="AC16QF_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448575.1"
                     /translation="MESKTPLADLLAYRDGEELPADVARGIEGDPAAQGELEKLRGIR
                     QQLNALPAVLPDPAVWEEISRRAQPPRSWTLRYPLATAATVFLAAALTIVAWNPLGNG
                     GAGAGPEPVVVADGALVDLMSRSQALESHLPVQGTPAGQLAWGSAEEALRYRIADIDA
                     QLMRIYETEPVDVERREALWRQRVDLLESLTQVQRGQAVVRPAIY"
     gene            complement(9215..9811)
                     /locus_tag="AC16QF_08185"
     CDS             complement(9215..9811)
                     /locus_tag="AC16QF_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6448576.1"
                     /translation="MASFAEIRLEPGVARGLKRGDRSAQGIAYRVLAPTVMGLACRIL
                     QDEGSAQEVVQDTFVDLIEHARTLKDAQAVVGWVRQVAVNHCLMRLRSPWNKRRDELD
                     ADPAGDVYVDMAEGAARTEGMQDIEKALSELEPETRLVIWLHDVEGYTHQEIAGLVGR
                     TASYSKSQMARGYQKLAKRFRGTEDGIENTASRSARVP"
     gene            complement(9870..11147)
                     /locus_tag="AC16QF_08190"
     CDS             complement(9870..11147)
                     /locus_tag="AC16QF_08190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6448577.1"
                     /translation="MKFGIFYEHQLPRPWDDGAEQRLFNEALDQVELADRLGIDYAWE
                     VEHHFLEEYSHSSAPEVFLAACSQRTKNIRLGQGIRQVIPQYNHPARTAEVIATLDLV
                     SQGRVEFGTGESSAELELGGFRIPVAKKRKIFMEATEQICNMLAMDPYPGYKGQYFEM
                     PCRNVVPKPVQRPHPPVWVACSQRETIRMAARLGIGALTFAFVDPMEAQSWVDEYYGI
                     IKSDQCVPIGHAVNANICMVSSFSCHHDRQTAVQRGLEGFEFFGYALGSLYGFGEHKP
                     GRTDLWTEFQTVRAPQVRNEVADFSEALLGARGGIGTPEDLRQHLRKFERCGVDQVTF
                     IQQAGMNRHEHICESLEIFAAQVMGEFKEREAEREERKMRELEPYIAAAMERKVRMKP
                     LADDEIPTFPALGRRITEEQEDAPSIYERPAAG"
     gene            11294..11896
                     /locus_tag="AC16QF_08195"
     CDS             11294..11896
                     /locus_tag="AC16QF_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6448578.1"
                     /translation="MTDRTFCYPRFVGNNDKPAANSRAELENRLARVFIEHGYEGASL
                     SRLATASGLGKASLYHHFPGGKAEMASVLLTRAIARLDKTAFSRLTKQQPAPVRLGRF
                     VDAFSSYVDHGERPCLIAVLDHGALRAVHGDTIRRRFLSWGERLAATFEETGMKPRRA
                     RRAASDLLAGLYGNLAMARMLDDASLFQRGTRRLKKDLPS"
     gene            complement(11893..13332)
                     /locus_tag="AC16QF_08200"
     CDS             complement(11893..13332)
                     /locus_tag="AC16QF_08200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase family protein"
                     /protein_id="HIR6448579.1"
                     /translation="MAKYDNNLIVIGAGSAGLIVALIAATVRARVTLIEQGAMGGDCL
                     NTGCVPSKTLIRSAKIAHYQATAQRYGVQAVTPQVDFQSVMRRVHEVIATIEPKDSMA
                     RYRELGVNCVAGAARLIDRHHVQVGDERLSARSIVLATGAEPVVPPIPGLADARPLTS
                     DNLWELESLPQRLLVMGGGPIGCELAQSFARLGSAVTLIDMAPGLMPREDDDVAGFVE
                     RRLRGEGIDVRTGHRAVRVVASADGAGVLHADSDGGEVQIGFDRVLVAVGRRANTAGL
                     GLEALGIATRSDGTLEVDAYLRTAVRNVYGCGDVVGPYQFTHMASHQAWYAAVNALFG
                     RFRKFKVDYSVVPWVTFTDPEVAHVGLSETEAAERGIRVEVTRYGLDDLDRALADGSA
                     SGWVKILTRPGSDRILGVSIVGAHAGELIAEYVLAMTHGIGLKKILGTIHVYPTLAEA
                     NKLAAGAWRRAHAPERLLRWVGRLHDLLR"
     gene            complement(13361..>13866)
                     /locus_tag="AC16QF_08205"
     CDS             complement(13361..>13866)
                     /locus_tag="AC16QF_08205"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR6448580.1"
                     /translation="VTALSLPGAAIMTLAAGAVFGLFWGTLITSFASSAGATLAMLIS
                     RTLLRDWVHARFAAQLRVVNDGLARDGAFYLFSVRMVPLFPFFIVNLVMGLTAIGTWQ
                     FYWVSQVGMLAGTLVFVFAGTQLANVDTVADVLSPGLIAALSLLGLFPLIARKTMGWL
                     ERARAAR"
BASE COUNT         2410 a         4713 c         4453 g         2290 t
ORIGIN      
        1 aattcgtaac ggaagcgtat ttcggcctca tccgcaattt ccggcgcttt tcgtggctgc
       61 tcatctacct gttcgggtcg tcgcctgcga tctgcaaatc gttcgtgaaa aatcagcccc
      121 atgagctggc gtccttcgac gaaggctcgc tctaccttcc acacgccacg tcgctgcgaa
      181 tggggcgcct gggctaccag agcgacgctc agcgcagcct ccacatttcc tacaacggtc
      241 tcgagcagta cgcggcatcc atgcacacgg cgcttacccg accttacccc ccgtacgagg
      301 ccatcggcgt caaagtcgac ggcgagtacc gacagctgaa cgcctcgctg ctgcagatcg
      361 agaacgagtt ctacggaacc atccggccga aacgccgcat cgtgagcggc gaaaggcccc
      421 tgaacgccct gagatcccgc ggggtcgagt acgtcgaggt cagatgcctg gacctgaacc
      481 cgtttctcca catgggcata gacaccgtcg agatccgctt catcgacgcg tttctgcttt
      541 actgcctgct ggcggacagc cctgaggaca gcgagcggga atcggagttg atggggaaaa
      601 accagctcgc cgtggtcgag cggggccgcg agccggacct tcaattggag cagctgcgcg
      661 gcaccatcac acgaaaggca tgggcggaag acgtcctgca cgcctgccgt ccgctcgccg
      721 agcttctgga cgaggccacc gggagcgaac tgtaccgcca cgcgctcagc gagcagtcca
      781 gcaagaaccg gcagccggcg ctgacgccgt cggcgcgggt gctggccgtc atgcagaccc
      841 agggcatacc gtttttccgc ttcgccatga accagtccat tgcccacaag ggttacttcg
      901 acgagcaccc gatgcgaggt gagcagctgg ccgagcacca ggccacctcg caacagtccc
      961 tggcgcggca gcaggagatc gaggccgccg acagcgtgga tttcgacgtc ttcctcaagg
     1021 actacctggc tctcgagtga actttctcgc ccactgcctg atcgctgcgc aatccaccga
     1081 ccccgaggaa ccggacctcg tcgccggcgg ctttctgggc gacttcgtca agggccccat
     1141 tggccccgaa ctgcccccgg gcctggctct cggcgtacgg ctgcatcggc gcatcgacgc
     1201 cttcagcaac cggcacccgg ccatccaggg gagctgcaat cgatttgcgg gccccctgcg
     1261 ccggctggcg cccgtgctcg tcgacatcat cgccgaccac tgcctggcca agcactggga
     1321 gcagttcagc aacgagccca tcgcagcctt caccgcaagg acctacgatc gcatcgcgtc
     1381 ccatgagcgc tggctccccg accacggcca ccgcttcctc agctacatgc gcgacgaaga
     1441 cctgctggcg gggtacgccc ggttcgaagc catgcaccgg gctctgtgct cggtgacacg
     1501 tcgcctggat cgcgagcacc tcaacgccga catgctcagc gctaccgaag caaacctgcc
     1561 cgggctggag gccgatttcc tggggtactt cccggaactg gtggcccatg cacggcagtg
     1621 gttgcgcgac gagggcccgc cccccggacc ccaaacgccg ggcacggtgc agcgctgaaa
     1681 ccggtactgc caggttgacg ctgccgaacc gaccgttact cttgcgttct ttgattttca
     1741 ggaagcatcc atgaaagcgg ttctgtgcaa ggcattcggc ccaccggaag acctggtgat
     1801 ggaggaggtg gcaaatccgg agcctgggcc cggccagatc ctcgtcgaca ttcatgcctc
     1861 cgccctgaac ttcccggacg tgctgatgat cgccggcaag taccagagtc tcccaccgtt
     1921 ccctttctct ccgggcggcg agatcgcggg aaccgttgcc gcggtcggcg acggcgccac
     1981 gcgtttcaag gtcggcgacc gggtctttgg agggaccggc cacggcggct tcgccgagca
     2041 gatcgccctg cccgagatgg ccatgcggcc catgccggac gagatgagct tcgtcgaggc
     2101 ctccgccatc agcacgacct acggcacctc ctactacgct ttgaagcagc gggccgatct
     2161 caagccgggc gagaccctgc tggtcacggg agcgggcggc ggcgtggggc tggcggccgt
     2221 ggagctcggc aaggccatgg gcgcacgggt aatcgccgcg gccagctctg ccgagaagct
     2281 cgccgcggcc aggagcgccg gggccgacga gctcatcgac tacagcgatg gggagctcaa
     2341 ggagaaggtc aaagcactca ccgacggcaa gggggcggac gtcatctacg agcccgtcgg
     2401 cggcgatctg ttcgagcagt gcatgcgctg cgtgaactgg tatgggcgga ttctggtcat
     2461 cggcttcgcg gccggtgaca tcccccgcgt acccatcaac ctcattctgc tcaagagctg
     2521 tcaggtggtt ggggtcttct acggcgcctg gagcgcgagg tgccgcgacg aggccgacgc
     2581 caacttcaag gagatcctgg acctgtaccg ggacggcaag atcaagccga tgatcggccg
     2641 tacctacccg ctggaggagt atgcggccgc gctgcgctgc ctgagcgagc ggcaggccat
     2701 cggaaaagtc gtggtcaccg ttcgatgaac gcctgggtct tcagagatac cagcgagctg
     2761 tgggcggtaa tgctcgtcgc cagcgtcggc gcgctcctgt ttgcggcgct gatcatggag
     2821 cacgtcttcg gcatggcgcc atgcccactc tgcctgatgc agcgaatctg gttcttcgtt
     2881 gccggcgcct tcgcgttcgc cagcctgctg cacaacccgc ggctggggat ctacccgctg
     2941 tgctccatgg tcgcggcggt gatcggcggc gggttctcga tccggcagct gtacctccag
     3001 ggcctcccgc cggaccaggt gccgtcctgc ggcccggaca tgagctacat gctcgaggcc
     3061 ttccccctca ccgacgtgct gatcgccatg acccggggaa cgggcgattg cgccgaggtc
     3121 gcctggagtt tcctgggaat ttccattccc ggctgggccc tggtggggtt tgccgctctg
     3181 gcggtgatgg cggcgctgca gctgcgcgca ggaacacgct gaccggtccc agggagccac
     3241 ctgccgctga gcaggcggcg cttgttgatt gcccgaggtg agctatgctg tgagcctcgc
     3301 tttaggagaa cgcagaatga gtcgggcgca gctgatcaac atggccaggc acaacatcgg
     3361 ccacgcgaaa gccggaacca tcgagcaggc tccggacatc gtcaaggtgc cggtgcagaa
     3421 ctacttcgac cccgatcgct ggcagctgga gatggatcgc atcttccgcc gcatgccgct
     3481 ggtgctggcg atgacggacg agctgcgcaa tccgggcgac tacaaagcca tggaagcggt
     3541 gggcatgccc gtgctcttga gccgcggcga ggacggcgag gtccgggcat tcgtcaacat
     3601 gtgccgacat cgcggatccc aggtgatgct gcccggatgc ggcaacgctc atcgtttcac
     3661 ctgtccctac catgcatgga cctacgacca ggagggagct ctggtcggcg tctacgcgcc
     3721 gaaggatttc ggcgacgtcg acaaggggga aaacggcctc gtgccccttc ccgtcgccga
     3781 gcgggcgggc atgatctggg tcatcgtcaa tccccaggcg accctggaca tcgacacctt
     3841 cctgtccgga tacgacgagc tgctcgggca ctttcacttc gaggactggc atctcttcga
     3901 gcagcgcacg atcgccggcc ccaactggaa gatcgcctac gacggctaca tggacctcta
     3961 ccacctgccg atcctgcaca aggacacgtt cggtgatcag tttcccaacc aggcgctgta
     4021 ctacgcctgg gggccccatc agcgggtcag ctcgccggac ccgacgcttg caggctacga
     4081 gcagctgccg gagtccgagt ggaacgaagc acacatgctg gcgggcgtgt ggaccatctt
     4141 tccgcacgtc tccgtcgccg gcttcgacgg cggcggccgc agcgtcatgg tgtcccagct
     4201 gttcccgggc aagacgcccc aggaatcggt gaccgtgcag aactacctga tggaaaaggc
     4261 gcccgacgcc gagcaggctg aggcggccga gcagatgttc aagctgctcg agtacgtggt
     4321 gcaggaggag gactacgcca ccggcctgcg gcagcagaaa gcaatcatga ccggcgccat
     4381 gagccacgtg atgttcggcc ggaacgaaga gggcgggcag cgcttccacc gctggctgga
     4441 caccctgctc gcaaccgatg acgaggacct caacgccctg tttgcgggcg gcgagcgtta
     4501 ctgctgaccg cgggcttccc ggacggcctc ccgggcgccg aagagccccg gcgagaggcg
     4561 cgtcatgcgg cgccaggggc cctgcaccat tggtgacatc cgcgcaaacg gtgttatatt
     4621 ccagcgctgc ccgtccggcc cggccgccag acaacggtcg acgccggcgt cctgggggac
     4681 gcatggagca gggggggcct gaaacgaggc ccagggacac agggatcccg attcgcagag
     4741 agctctcaaa gaaagggccc cagcgcatga atgcgaacgt tcggcgcata ctgatcagca
     4801 acggcaaagg cgggtgcggc aagaccacca tcgccgccaa tcttgccgtg tcatacgcca
     4861 atcgcggcgc caaggtcgca ctggtcgacc aggatccgca ggcgtccagc agctattggg
     4921 cggaccagcg ctcccgggag cttgcacccg ttcacctcgt ccgcgcgcat cagcgcgcca
     4981 acatgtatca gactcagacc tttcacaacc ggatacccgc cgacatcagc cgggtcgtcg
     5041 tcgacggcag caccaaccca caggacagag aactggagat gctcctgaag cagtgcgacg
     5101 tgattctggt tccgctgctg ccgtcggcca tcgacattcg agccggcagc cagttcatca
     5161 aggagctgat cacccaccgg agcttccgga cgctgcccaa gcccgtgggc gtcatcgcca
     5221 accgggttca gccgaactcc accatgcacg cgcggctgga gcacttcctc aagtgcctgg
     5281 acgttccgtg ggtcgccacc ttccgtgaca gccccctcta tccggaagcc ttcgagcggg
     5341 gagagggcgt cgtggacctg atcgactgcc gggccgcgcg ccgggaagcg cccgcgtggc
     5401 gagcgctcac ggcctggatc gagcggcagc ccgttagccg aggttgcacg gcccgggagc
     5461 tgcgcaaccg accgatccgg gcgccgcgcc acgtcgacga cgagaccttg agcgcataga
     5521 atccggcgcc agccagcttc tcgggtcttc cgaaaatcag gcccggcgcc acctcatctg
     5581 atcacgttct gcgcgtcatt ttcacagagc cgccccaccg atgctccagc tgatcgacat
     5641 ttccttgaag cgcggctcaa accagctgtt cgagcacctg acgtgcacga tccacccagg
     5701 ccacaaggtc ggcatggtcg ggcgcaacgg cgcgggcaaa tcaactctgt tcgacatgat
     5761 cctcggccgc ctgcagcccg acgagggaga ggtgagccgc ccctctggct gggtagcctc
     5821 ccacatggcc caggaagtca acgtcaccga tcggccggcc atcgagcacg tgatcgacgg
     5881 ccaccgggag ctccgagccg tggagcgccg catcgcacag gccgagtccc ggcgcgatga
     5941 cctgacgctg gcggagctac atacgcgctt cggcgacgtc ggcgggcacc aggcacgggc
     6001 ccgcgccggg actatcctgc acgggctcgg ctttgccgcc gatgagatcg agcagccgtt
     6061 tcgagctttc tccggcggca tgcggatccg cctgaatctg gcccaggcgc tgatggctcc
     6121 ggccgatctg ctgcttctgg acgagcctac caaccacctg gacctggaga ccacggtctg
     6181 gctggaatcc tggctgcagc gctttgctgg aaccctgctc gtgatcgcgc acgatcgggc
     6241 cttcctcgac ggcgtgtgcg accacatcgt tcacatcgaa cacgggcgcg cgaactccta
     6301 ccggggcaac tactcctcgt tcgagcggca acgagcggaa gcgctggcgc agcagcaggc
     6361 ggcccacgcc aggcagcagg ccgagatcaa gcacatgcag catttcatcg accggtttcg
     6421 cgccaaggcc agcaaagcga agcaggcaca gagccgaatg agggccctgg agcgcatgca
     6481 ggccgtagcc cccgtgcatg ccgactcgcc ctaccgcttc agcttcgcga atccggagca
     6541 gatgtcccac cccctgctga gcctggagga cgtgtccgtg ggctacgaag gacgcgcggt
     6601 gatcgagcac gtcaggcaga cgcttctgcc cggcgcccgc atcggcatac tgggagccaa
     6661 cggcgccggc aagtccaccc tgctcaagtg tctggtgggc gagctcgagc ccctggcggg
     6721 cacgctggtg cgcggacgcc actccgcggt cgggtacttc gcccagcatc agctcgaacg
     6781 gctggacggg cagacgtccg ctctggcgac cctcacgaac gagcggccac aggcccggga
     6841 gcaatggtgc cgggactacc tgggccgctg gggcttcccc gccgacatgg tggagcggcc
     6901 catcgttacg ctttccggcg gcgagaaagc ccgtctcgtg ctggccctga tcgctctcgc
     6961 ccagccggcc attctcgtcc tcgacgagcc gaccaaccat ctggacctgg aaatgcgcga
     7021 ggccctggcc ctggcgttgc aggactatgc gggcgccctg ctgctggtct cccacgatcg
     7081 cagcctgctg cggcgggccg tggacgagtt ctggctggtt gaggggggca ggcttgcgag
     7141 tttcgccggc gatatcgacg cctacgccga gagccgtctg aggcgaagcg aacctccgtc
     7201 atccgaacgc gctcccgaga cgaaccgcaa agccctgcga caggcggcgg ccgcccagcg
     7261 ggaggcggcc aagccgttgc gggcaaagct caggcacctg gaaaaggaga tggatcagat
     7321 cggcgtggag ctgcgcgagg tggagtcccg actggcggat ggggacgtgt accggagcct
     7381 gcctccggag gagctcgacg acctgctctc cgctgccggc aggctgagaa aaagattgga
     7441 cgcgaccgag caggcctggc tcagcgcaac cgaggcgctc gaagcgctcg agcagagctg
     7501 aacggcgcgc ccggagggga tgagggtcgg tccggacgcc tagccgcccg ccgaatcgtc
     7561 ggcggctgct tttttagagc ctttctcgtc cacgaccacc tcctcgtcca cgacgatccg
     7621 aacgtcaact ccatcgtcgc cgtcgatgcc acgccgaatc cggatgatgc tcggatcgat
     7681 cgtgatggcc atgtgatcgc ccgcggagaa ctgcgagcag tcgatggaca cctcgcgttt
     7741 cttgccgttg cgcagaaccc cggcgtcgat ggtcccgtca ctcatggcga gcgtcttcat
     7801 ggcttcccgg gcgttggcca cgtccttgcc gtcgagctcg agcagcacgt ccccactctt
     7861 gagctcgcct tcctgtgaag gcggagcgac cacgaggacg cctgcgtcga ctccgaaata
     7921 cctggccagg tcgccgtcca tgtcttcgag cttcaaggag gtgcccacgt cgacgacgtt
     7981 gccgatcagc tcgcccagat ctccgagctc gtgaagagcc tccagcttgc cgagttcttt
     8041 gagttccttg agctctccga ggtcctcgaa ctctttgatg agctcggccg tgaactcggg
     8101 atcgagctga tccagctcca tcgccaggga cttcatcacg aaggcacgtt tgggctgcgt
     8161 gaccacgtcg gctttcagcg tctcgccgtc gcgcacgtat tccaccttca cggggtcgcc
     8221 gggctcgagg tccttgacca cgttgctgag gagcttgccg ggtttgtccg caccgccggt
     8281 gtccagccgc acgccgccga tggcgacgat ccgatcgccc gcggccagtc cagcctgctt
     8341 cgcgccgcca tcgggcgtca cgccagcaat caccaggcca tcgtcgtcgc ccatgctcgt
     8401 cagaaggata ccgatcatcg cgcgtttccc gtagcgaccc tctgaaacct cggcttcata
     8461 catctgccga tgcagctcgg cgagccgctc ggcggcggca tcgagctgtt cgcgggcctc
     8521 gcgcagccgg tcctccagct cgtcggcaaa ggtatatccg gagagcagca gtgccatcgc
     8581 cgtgaacccg gcgagcacct gccgcttccg cgagctcaac cacttgatca tcatcgactc
     8641 ctagtaaatt gcaggtcgca ccacggcctg gccgcgctgc acctgagtaa gcgattcgag
     8701 caggtcgacc ctctgccgcc acagcgcttc acgacgctcg acgtccaccg gttcggtctc
     8761 gtagatccgc atgagctgcg catctatatc ggcaatccgg tacctgagcg cctcctcggc
     8821 agagccccag gccagctgac cggcgggcgt gccctgcacg ggcaggtgcg actcgagagc
     8881 ctgggatcgg ctcatcaggt caaccagggc accatcggcc accacgacgg gctctggacc
     8941 ggcgccggct ccgccgttgc caagggggtt ccaggcgacg atggtgaggg ccgccgccag
     9001 aaagaccgtt gccgcggtcg ccagggggta gcgcagcgtc cagctgcgcg gcggttgcgc
     9061 cctgcggctg atctcctccc acaccgccgg atcgggaaga acggctggga gcgcgttgag
     9121 ctgctgcctt atgccacgca gcttctccag ctcgccctgg gcggcagggt cgccttcgat
     9181 gccgcgcgcc acgtcggcgg gcaactcctc gccgtcacgg tacgcgagca gatcggctag
     9241 cggtgttttc gattccatct tccgttcctc tgaatctctt cgcgagcttc tggtagcctc
     9301 gcgccatctg ggacttcgag tagctggcgg tgcggcccac cagccctgcg atctcctggt
     9361 gggtatagcc ctccacatcg tggagccaga tcaccagccg ggtctccggc tccagctcgc
     9421 tcagcgcttt ctctatgtcc tgcatgccct ccgttcgggc cgccccttcg gccatgtcga
     9481 cgtatacgtc ccctgccggg tccgcatcga gctcgtcccg ccgcttgttc cagggggaac
     9541 gcagccgcat caggcagtgg tttacggcaa cctgccgcac ccagccgacg accgcctgcg
     9601 cgtccttgag cgtgcgcgcg tgctcgatca gatccacgaa ggtgtcctgc acgacttcct
     9661 gagccgatcc ctcgtcctgg agaatgcggc aggcgaggcc catcacagtg ggcgcgagca
     9721 cacggtaggc gatgccctgg gcactgcggt caccccgctt gaggccccgg gcaacgcctg
     9781 gctccaggcg aatttcggca aaactggcca taccactcta tagatgcgtc agacccgcaa
     9841 agggtcgcag caacggagat ttttttcggc tacccggccg ccgggcgctc gtagatggac
     9901 ggggcgtcct cctgttcctc ggtgatccgc cgtccgagcg ccgggaaagt cggtatctcg
     9961 tcgtccgcga gcggcttcat cctcaccttg cgctccatgg cggcagcgat gtaaggctcg
    10021 agctcacgca tcttgcgctc ctcccgctcc gcttcccgct ccttgaactc tcccatgacc
    10081 tgcgcagcga agatctcgag agactcgcag atgtgctcgt gccggttcat tccggcctgc
    10141 tggatgaagg tcacctgatc cacgccgcag cgctcgaact tgcgcagatg ctggcgcaga
    10201 tcctcagggg taccgatacc gcctctggcg cccagcagag cctcgctgaa gtcggccacc
    10261 tcgttcctga cctgaggcgc gcgcacggtc tggaactccg tccagagatc cgtgcggccc
    10321 ggtttgtgct cgccaaagcc gtagaggctg ccaagggcgt agccgaagaa ctcgaagcct
    10381 tccaggccgc gctgcacggc ggtttggcgg tcgtggtgac aggagaagct ggacaccatg
    10441 cagatgttgg cgttgacggc gtgaccgatg ggcacgcact gatcgctctt gatgatgccg
    10501 tagtactcgt cgacccagga ctgagcctcc atggggtcga cgaaggcgaa ggtcagcgcc
    10561 ccgatgccca gccgggcggc catccggatg gtctcgcgct gagagcaggc cacccagacg
    10621 ggcggatggg gccgctgcac gggcttggga accacgttcc ggcagggcat ctcgaaatac
    10681 tggcccttgt acccgggata cgggtccatg gcgagcatgt tgcagatctg ctcggtggcc
    10741 tccatgaaga tcttgcgctt cttggccacg ggaatccgga agccgccgag ctcgagctcc
    10801 gccgaggact cgcccgtgcc gaactccacg cggccctggg agacgagatc cagggtggcg
    10861 atcacctcgg ccgtgcgggc cggatgattg tattgcggga tcacctgccg aatgccctga
    10921 cccaggcgga tgttcttggt gcgctgggaa cacgccgcca ggaagacctc gggcgccgag
    10981 gagtgggaat actcctcgag aaagtggtgc tcgacctccc aggcatagtc gatgcccagc
    11041 ctgtccgcga gttccacctg gtccaacgcc tcgttgaaca gccgctgctc cgcgccgtcg
    11101 tcccagggcc gcggcagctg atgctcgtag aagatcccaa atttcatgtt catgtcccca
    11161 ctgtgttgtg cagatccccg gcggccgggc ggccagggcc ccgaatggcg ctttcccgct
    11221 gcccgagtct cccctcaaag ggcacgtcgc atcaaggggc accgcgccag cggcgcggat
    11281 cagacggcgc cttttgaccg accgaacgtt ctgttaccct cgtttcgtgg gcaacaacga
    11341 caagccagct gcgaatagtc gcgccgagct cgaaaatcgg ctcgccagag tcttcatcga
    11401 gcacggctac gagggcgcca gcttgagccg cctggcgacc gccagcgggc tggggaaagc
    11461 cagcctctac catcactttc ccggcggcaa ggcggagatg gcgtcggtgc tcctgaccag
    11521 ggcgatcgcc cggctggaca aaacggcctt ttcccggctg accaagcagc agcctgcccc
    11581 ggttcgtctc ggccggttcg tggacgcttt cagcagctac gtggatcacg gcgagcgccc
    11641 ctgtctgatc gcagtgctgg accacggcgc gttgcgggcc gtgcacggtg acaccatccg
    11701 ccggcggttt ctgagctggg gcgaacggct tgccgccacg ttcgaggaaa ccggcatgaa
    11761 accgcgccgg gcgcgtcggg cggcttccga tctgctggcc ggactgtacg ggaacctggc
    11821 aatggccagg atgctggacg atgcgtcgct gttccagcgc ggcaccaggc gactgaagaa
    11881 ggaccttcct tcctagcgca acaggtcgtg gagcctgccg acccagcgta gcagccgttc
    11941 gggcgcgtgg gcgcgccgcc atgcccccgc agcgagcttg ttcgcctcgg cgagggtcgg
    12001 gtagacgtga atggtgccga gaatcttctt cagcccgatg ccatgggtca tggccagcac
    12061 gtactcggcg atgagctcgc ccgcatgggc tcccacgatg gaaacgccca gaatgcgatc
    12121 cgagccgggt ctggtaagga tcttcaccca acccgaggcg ctgccgtcgg cgagcgcccg
    12181 atccagatcg tccagaccgt accgagtgac ctcgacccgg atgcctcgct cggcggcctc
    12241 ggtttccgag agcccgacgt gggcgacttc cggatccgtg aacgtcaccc agggcaccac
    12301 cgagtagtcg accttgaact tgcggaaccg gccgaacagc gcgttgaccg ccgcgtacca
    12361 ggcctgatga gaggccatgt gagtgaactg gtagggaccc accacgtcgc cgcagccgta
    12421 gacgttacgc accgccgtgc ggagataggc gtccacctcc agcgttccgt ctgaccgggt
    12481 ggcgatcccc agcgcttcga gccccagccc cgccgtgttg gcgcggcgcc cgacggcaac
    12541 gagcacccga tcgaagccga tctgcacctc gccgccgtcc gaatcggcat gtagtacccc
    12601 ggcgccatcc gccgaggcca ccacccgcac ggcgcggtgg ccggtgcgga cgtcgatgcc
    12661 ctcaccgcgc agcctgcgct cgacgaagcc ggcgacgtcg tcgtcctccc ggggcatcag
    12721 gccgggcgcc atgtcgatga gcgtgaccgc cgagcccagt cgggcgaaag actgagccag
    12781 ctcgcagccg atcggccctc cacccatcac cagaagccgc tggggcaggc tctcgagctc
    12841 ccagagatta tcggaggtga gcggccgggc atcggcgagc ccgggaatgg gcgggacaac
    12901 gggctcagcc ccggtggcga gaacgatgct gcgggcgctc aggcgctcat cgccaacctg
    12961 cacgtgatgc cggtcgatca ggcgcgccgc tccggccacg cagttgacgc cgagttcccg
    13021 gtagcgggcc atggagtcct tgggttcgat ggtcgcgatg acctcgtgta cccggcgcat
    13081 gaccgactga aagtccacct ggggagtcac cgcctgcacc ccgtagcgct gggcggtggc
    13141 ctgataatga gcaatcttgg ccgagcggat cagcgtcttg ctcggaacgc accccgtgtt
    13201 gaggcagtcg ccgcccatgg cgccctgctc gatcagggtg acccgggccc ggaccgtggc
    13261 cgcgatcagg gcgacgatga ggccggccga gcctgcgccg atcacgatca ggttgttgtc
    13321 gtacttcgcc atgccctgtg acccccgcgg aaactgcagc tcatcgcgcc gcccgggcgc
    13381 gctcgagcca tcccatggtc ttgcgcgcga tcagcggaaa caacccgagc aagctgaggg
    13441 ctgcgatgag ccccgggctg agcacgtcgg caacggtgtc cacgtttgcc agctgagtgc
    13501 ccgcaaatac gaagacgagc gtgccggcca gcattcccac ctggctgacc cagtagaact
    13561 gccaggtacc gatggcggtg agccccatga ccagattgac gatgaagaac ggaaacagcg
    13621 gcaccatgcg cacgctgaaa aggtagaacg cgccgtcgcg tgccaggcca tcgttgacca
    13681 cccgaagctg ggcggcaaag cgcgcgtgca cccagtcgcg caggagcgtg cgggagatga
    13741 gcatggccag ggttgcgccc gcgctgctgg caaaggacgt gataagggtg ccccagaaca
    13801 ggccgaacac ggcccctgca gccagcgtca tgatcgcagc gccaggcagc gagagcgccg
    13861 tgaccg
//
LOCUS       DBNHLM010000179        12478 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_624856, whole genome shotgun sequence.
ACCESSION   DBNHLM010000179 DBNHLM010000000
VERSION     DBNHLM010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12478)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12478)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12478
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_624856"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="AC16QF_08210"
     CDS             <1..453
                     /locus_tag="AC16QF_08210"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003152943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase domain-containing
                     protein"
                     /protein_id="HIR6448581.1"
                     /translation="GSVSFDHPDPSIYTVLTSPSETAGMANADFVIFPPRWIVAEDTF
                     RPPWYHRNVMSEFMGLVRGLYDAKETGFVPGGCSLHNSMAAHGPDVEAFEKASSADLK
                     PQKLADTLAFMFESRYPYTATAFAMESPALQSDYRACWAALPRMFEPQ"
     gene            499..1707
                     /locus_tag="AC16QF_08215"
     CDS             499..1707
                     /locus_tag="AC16QF_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6448582.1"
                     /translation="MVPTETEIREFDLRNPPAGFVEDPFFWYEALRAGGPVRRMPDGS
                     MLVTGYDACSDIYRNEAFISDKRNLFRPKFGDSPLYEHHTTSLVFNDPPYHTRVRRTI
                     VEALKPRSIQPTLDMLARRIQELLASMGEAGEADLIEAYASRIPVEVICSLLTVPTQD
                     RDDLRRWSLAILGALEPAISESEAEQGNRAVTEFLGYLRTLIAERRRRPLAEDSVLSS
                     LLAQQDRGEVSELELLHNCIFLLNAGHETTTNLIGNGVHMLLTHDSERDLLVAHPSRI
                     KSAVEEILRYQSPNQLGNREVTGNVTMCGQRFAPGDQVTLCIAAANRDPRKFPDAHRF
                     LIDRAPNPHLAFAAGPHTCAGMALARIEGKLALSAFFQAFPEATLQARPRYRDRIRFR
                     GLEALHVRLQ"
     gene            1792..2691
                     /locus_tag="AC16QF_08220"
     CDS             1792..2691
                     /locus_tag="AC16QF_08220"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6448583.1"
                     /translation="MTEAFDVVGDWQVARLAGALGAEVRGIDLRQVTSEEVDRIRSLL
                     LDHLVLFFPDQNISVDDHVSFGRYFGELEGHPHLDNPFTEHPELFELAASRGGVADEW
                     HTDITFRDEPALMSILHMVRCPTVGGDTMWSSLYAAFEELSEPMRDLCEGLTALHDAL
                     PHGRPEQTAIHPVVRVHPETGRKALYVNEHFTRRIVEMNARESDMLLGYLTKWVQNPR
                     FTVRYRWAEGTIAMWDNRCTQHFVLNDFEGERVIQRVTVMGDRPEGSGARWAPAVRTG
                     RHSATSRYDAQLVRHLRSREDQA"
     gene            complement(2791..4344)
                     /locus_tag="AC16QF_08225"
     CDS             complement(2791..4344)
                     /locus_tag="AC16QF_08225"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6448584.1"
                     /translation="MTDPLTELARSQPDKPALIDDRPDGTLLKWTYSELEERTNALAR
                     VFQNAGVEAGNRLIWCGPNSAWVVAAGIAARKIGAVAVPLNYRLTAEEAAYVVDNSDA
                     VTVFADAEYADLFAEIRDDIPQVREVLIFDGPAGPGQTSAEERLAQEGTEELAVKVDP
                     TTSGTMIYTSGTTGRPKGAVRKDTGDPALIAALMELMQLRGDDVYLTTGPLYHSGPSG
                     FMAFAFAFGNTIVLQRKFDAEDWLRLIDKYGVSFTFSAPTPVRLATQLPPETIKRYDL
                     SSVRITIANAAPWSFALKKAYLEHFPADSLFEVYGSTELGVNTIMLPADQLRKPGSCG
                     QAAPGVEIILIDEEGREVTEPKVPGEVFIRSPSVFSTYHKAEDKYLEDRRGDMQTVGD
                     IAYRDDEGFYYICDRKKDMIISGGVNVYPAEIEAALEHHPDILDVAVFGIPSEEWGES
                     VHAVVVKRSGSGLNEEAIQAFAREHLAGYKVPRSIEYRDAIPRTGTGKILKRELRAPW
                     WEGHNSSVS"
     gene            4486..5778
                     /locus_tag="AC16QF_08230"
     CDS             4486..5778
                     /locus_tag="AC16QF_08230"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6448585.1"
                     /translation="MASNPQSGDVSDIQLFDVETLKDPWDAYARLRDHAPVFFVPGFD
                     IHVVTRYDLVREVIKDTETYSSKFDLFLNRSRELMFASAPPEVQAELIRLNDEMIQIP
                     PTMLTLDEPEHTKYRSLVSQLFTVSQIRKAEDAVQAVIDETLETFVDRRSVDFMDAFA
                     FPVPLRIIADRLGIPEDDRAFFDDAATAAAAGLRLSPITPEEMVRRAQLGVDLQKLLV
                     RIIEERRTDPLEDMISILANSKLEAEDRYLTHGECLSILNQFLVAGHETTTSTFGWGM
                     LLLCRNPEAQRALRADPSLTRTFVEEALRLEAPVQGLPRLVTKDTELGGVELEAGKLI
                     MIRYGAANRDGRQFENPDELDLHRKKAGMQLAFGSGVHHCIGAPLARQELNLGFPTLL
                     RHMSEIRLSADHPPPEAEASFILRNLPELHIDYDLIEP"
     gene            5800..6822
                     /locus_tag="AC16QF_08235"
     CDS             5800..6822
                     /locus_tag="AC16QF_08235"
                     /inference="COORDINATES: protein motif:HMM:NF028222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR6448586.1"
                     /translation="MKAALMENGRIWVDTVPDPEPRLGEVLVKSAGCGICGSDLHAYR
                     HTEEFVATSRESGGAFRLTTMNPVVLGHEFCAEVVDYGPGTQRRLPVGTLVCAIPALP
                     RGGGFEPIGYSEDAPGGFAEYFLLNERLMMPVPPGLSWELAALTEPMAVGHHAINKAR
                     LDGEAVLVIGAGPVGLAVIANLKQRGVGPVVVAELSPGRRALAERLGADVVVDPAAGS
                     PYAVPEVSRAKGLVIFECVGVPGMIGQIFLDAPREAKIVVVGVCLQQDVSRPLIAINK
                     ELNVQYVLGYSIDEFTESLNMIADGTLDVEAMITHRIALEDVPRAFDDLGSPEVQGKV
                     LVQPWA"
     gene            6923..7243
                     /locus_tag="AC16QF_08240"
     CDS             6923..7243
                     /locus_tag="AC16QF_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR6448587.1"
                     /translation="MPKITFITHDGVEHVVDASNGASVMNAAIDNLVPGIDADCGGEC
                     SCATCHVYVDPSWTDVVGGPGDREEEMLDLNPERESNSRLSCQIPVRDELEGLVVRVP
                     EFQM"
     gene            7255..8502
                     /locus_tag="AC16QF_08245"
     CDS             7255..8502
                     /locus_tag="AC16QF_08245"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6448588.1"
                     /translation="MSEAIYENPNVLEQDPWQLPLEDINPAQAELFENDCHWAYFERL
                     RRDNPVHYCRDSIFGPYWSVTKYADIKYVDTNHEIFSSEPTIVLDDQDEDFPLPMFIA
                     MDPPKHDRQRKEVAGVAGPRNLAALEPTIRQRVCSILDSLPVREKFNWVDLVSIELTT
                     QMLATLFDFPFEDRRKLTRWSDVATAPIGVLVDTDEARQAELLECLEYFTGLWNERVN
                     QPPGNDLVSMLAHGESTKNMEPMEYLGNLVLLIVGGNDTTRNSISGGVLALNQNPDQY
                     EKLRDDLSLIPNMCSEIIRWQTPLAYMRRVAVEDTDLGGQRIKAGDKVAMWYVSGNRD
                     PDMFDDPDAFIIDRANARKHIAFGFGIHRCMGNRVAEMQLRILWEEILRRFHTVELVG
                     EPVRVKSSFVKGYLDLPVILHPN"
     gene            8582..9031
                     /locus_tag="AC16QF_08250"
     CDS             8582..9031
                     /locus_tag="AC16QF_08250"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6448589.1"
                     /translation="MSEPDTSCSLVVSRELFVPRELVFDVWTHADHLENWYSPGPDFD
                     RHAEVDAREGGAYLLTWTGLDGRAYGQRGRFDSFHVPDKLSYTSIFEVDGEERISTRT
                     HVSFADLGGGTRIDIVVDGYPDGGSRDAHQASWALFLDQLEAYFSVI"
     gene            complement(9044..9373)
                     /locus_tag="AC16QF_08255"
     CDS             complement(9044..9373)
                     /locus_tag="AC16QF_08255"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6448590.1"
                     /translation="MNTSAWQVFHVDDLKSKLKGGAVEYKEFLRVPSVSCGLYHLAAG
                     SQDMQTPHDEDEMYFVVDGRARLKVGDEIYEVRPGSVLYVQATEEHSFFEIEEDMTLL
                     VFFASTS"
     gene            complement(9432..10937)
                     /locus_tag="AC16QF_08260"
     CDS             complement(9432..10937)
                     /locus_tag="AC16QF_08260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6448591.1"
                     /translation="MGQSIAIVGSGICGMCTAMTLAGQGHDITVFDKDVSPPAGNAEE
                     AFFSWERRGAAQFRHPHAFLGLMCNLIEERYPELLDAFYAAGARRVDLQEMLPAELRE
                     SYRPVRGDEKLWVLLCRRATIETIIRQYVGALPGIRIVNPCKVTDLITDSEGGRLQVK
                     GLMIQREHEGEVTPFQADVVIDASGRRTRFPRWFAQRGCTIPEEKHDAELVYYTRHYR
                     LKEGRSEPARSDRSGAGDLGYLKFGVFPGDNGHFAIILCVPTPETAIRKAVRSGDLFD
                     RICLSIPGLEPWLADGRAEATTDPFGIGDINAVWRNFVRDGQPLAVNFFAIGDAAVRT
                     NPLYGRGCSTGLLHASILTDVLAETSDPVERALAFHQRTVSELRPIYDASLGEDRRGI
                     KRATASMAGGLLEPPRTLRRWFALAFGDALSAASREQAHVLRGALRSFHLLEKPGEFL
                     NDWRIRRTVLRYMLRGRKRNATMRLQPGPERVELHELLGLQAPEPTAEPAP"
     gene            complement(10950..11831)
                     /locus_tag="AC16QF_08265"
     CDS             complement(10950..11831)
                     /locus_tag="AC16QF_08265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6448592.1"
                     /translation="MIEKIPFGRTGHESTRTLFGAAALGSMSPERAEGVLATLLEHGV
                     NHIDTAASYGDSELRLAPWLAANRASFFLATKTDQRTGSAARAQLERSLERLGVDQVD
                     LIQMHNLVDEQEWETAMAPGGALEALIDARAEGLVRFIGVTGHGTRVAAMHLKSLEQF
                     DFDSVLLPYNFTMMQQSDYAADVEALLKICKSRNVAVQTIKSVARRRWTDDDADKRFS
                     WYMPLRDRESLQRAVHFVLSRPGLFLNTSSDATILPLILEAASQPAEAPDPDAMRCDV
                     NALGMEPLFVRGVSDTI"
     gene            complement(11905..>12478)
                     /gene="larE"
                     /locus_tag="AC16QF_08270"
     CDS             complement(11905..>12478)
                     /gene="larE"
                     /locus_tag="AC16QF_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HIR6448593.1"
                     /translation="KPDYRANPIDRCFHCKSSLYAALTAIAREEGYAMIFSGTNCDDL
                     GDHRPGLKAADDHGVTAPLVEAGFGKADIRALADELGLDNAQKPQSACLSSRFPYGTA
                     IDENRLAQVEQAEDVLASFGFTQFRIRHHGDVARLELLPDEIPAALDRAADIESAIRA
                     CGYRFVAVDLRGFRSGSLNEGMVKVVNLPA"
BASE COUNT         2221 a         3904 c         3984 g         2369 t
ORIGIN      
        1 ggctcggtga gcttcgatca tcccgatccg tccatctaca cggtgctcac ctcgccctcc
       61 gagaccgcgg gcatggcgaa cgcggacttc gtgatctttc cgccgcgctg gatcgtcgcc
      121 gaagatacct ttcgtccgcc ctggtaccac cgcaacgtca tgagcgagtt catggggctg
      181 gtgcggggtt tgtacgacgc caaagagacc ggcttcgtgc ccggcgggtg cagcctgcac
      241 aacagcatgg cggctcacgg tcccgatgtg gaggccttcg agaaggcgag ctcggcggat
      301 ctgaagccgc agaagttagc cgacacgctg gcgttcatgt tcgagtcccg ctatccctac
      361 acggctaccg cgttcgccat ggaaagcccg gccctgcaga gcgattaccg agcctgctgg
      421 gcggcgctgc ctcggatgtt cgagccgcag tgatgcgcac ggggatggcg gccggcccac
      481 gggcggggtg agatgccgat ggtgccgacg gagacggaaa ttcgtgaatt cgacctgcgc
      541 aacccgccgg ccgggttcgt agaggatccc tttttctggt acgaagcgct tcgcgccggc
      601 ggtcccgtcc gccggatgcc cgacgggtcg atgctggtta ccggatatga cgcgtgctcg
      661 gacatctatc ggaacgaggc gttcatttcg gacaaacgaa acctcttccg gccgaagttc
      721 ggagactcgc cgctgtacga gcatcacacg acgagtctgg tcttcaacga ccctccgtac
      781 cacacgcggg ttcggcggac catcgtggag gcgttgaagc cgcgctcgat ccagccgacc
      841 ctagacatgc tcgcccggcg cattcaggaa ctgctcgcat cgatggggga ggcgggagag
      901 gcggacctca ttgaagcgta cgcgtcccgg attccggtgg aagtcatctg cagcctgctc
      961 acggtgccga cgcaggacag ggacgacctg cggcgctggt cgctggccat tctgggtgcg
     1021 ctggagcccg ctatctccga atccgaggca gagcaaggca accgcgcggt aacggaattt
     1081 ctggggtatc tcaggacgct gatcgccgag cgtcggcgcc gccccctcgc cgaggacagc
     1141 gttctgtcct cgctcctggc tcagcaggat cggggcgagg tgagcgagct cgaactgctg
     1201 cacaactgca tctttcttct gaacgccgga cacgagacca cgaccaatct gatcggcaat
     1261 ggcgttcaca tgctgctgac ccacgactcc gaaagggacc tgctggttgc gcatccttca
     1321 cgaatcaagt cggcggtgga ggagatcctg cgctatcaaa gccccaacca gctcgggaac
     1381 cgtgaggtga cggggaacgt aacgatgtgt gggcaacgct ttgcgccggg tgatcaggtg
     1441 accctgtgca tcgcagcggc gaatcgcgac ccgcgcaaat ttccggatgc ccatcgtttc
     1501 ctgatcgatc gcgcgccgaa tccccacctg gcctttgccg cgggtcccca tacctgcgcg
     1561 ggcatggccc ttgcgcgaat cgagggaaag cttgcgctca gcgcattttt ccaggcgttt
     1621 cccgaagcga cccttcaggc gcggccacgg tatcgtgatc ggatccgctt ccgcggcctg
     1681 gaagcgctcc acgtccgctt gcagtgaacg cggcacaagg ggccttggcg gcttgcaggc
     1741 ccggaagcgt tatatactct gcctacgtga cgcggaatct gatggacgat catgactgaa
     1801 gctttcgatg ttgtcggcga ttggcaggtg gcacggctgg ctggcgccct gggcgcggag
     1861 gttcgcggaa tcgatcttag acaggtgacg tcggaggagg tggaccggat ccggagcctg
     1921 ttgctcgacc acctggtcct gttctttccc gatcagaaca tcagcgtaga cgaccacgtc
     1981 tcgttcggac ggtatttcgg tgagctcgag ggccatcccc atctggataa cccgttcacc
     2041 gagcaccccg agctcttcga gctggcagcg agccgcggcg gcgtcgccga cgagtggcac
     2101 acggacatca cttttcggga cgagcccgcg cttatgtcga ttctgcacat ggtgcgatgc
     2161 ccaaccgttg gcggggacac catgtggtcg agcctttacg cggcgttcga ggaactctcg
     2221 gaacccatgc gcgatctctg cgagggcctc acggccctgc atgacgcgct gccccatggc
     2281 cgcccggagc agacggccat tcatccggtg gtccgcgtgc atccggaaac cgggcgcaag
     2341 gcgctttacg tgaacgagca tttcacccgg cgaatcgtgg agatgaacgc cagggaaagt
     2401 gacatgctgc tgggctatct cacgaagtgg gtgcagaacc cccgtttcac cgtgcgctac
     2461 cgctgggcgg agggaaccat cgccatgtgg gacaaccgct gcacccagca cttcgttctg
     2521 aacgatttcg aaggggaacg cgtcattcag cgggtgaccg tgatgggaga ccggccggaa
     2581 ggcagcgggg cgcgttgggc tccggcggtc cgcacgggcc ggcactctgc caccagccgt
     2641 tacgacgccc agctcgttcg ccatctgcgc agccgggagg atcaggctta gcgccggccg
     2701 cgcctgggcc gcttccggtg cagggtcgtt cgcctctttc gccttggggc acccgccgcg
     2761 cgatggtctt cacgtcaccg ggctgcggtt ctacgaaacg ctgctgttgt gcccttccca
     2821 ccagggcgcg cgcagctccc gcttgaggat cttgccggtt cccgtgcgag ggatcgcgtc
     2881 gcgatattcg atcgagcgcg gaaccttgta gcccgccagg tgctcccggg cgaatgcctg
     2941 aatggcctcc tcgttcaatc cagagccgct gcgtttcacg acgacggcgt gcacgctctc
     3001 tccccactcc tcgctgggga ttccgaacac ggcgacgtcg agtatgtcgg ggtgatgctc
     3061 cagcgcggct tcgatctccg ccggatagac gttgaccccg ccggagatga tcatgtcctt
     3121 cttgcgatca cagatgtagt agaagccttc gtcgtcgcga taggcgatgt cgcccaccgt
     3181 ctgcatgtcg ccgcggcggt cttccagata cttgtcctct gccttgtgat aggtggagaa
     3241 cacgctcggc gagcggatga acacctcgcc ggggactttt ggttccgtga cctcccggcc
     3301 ttcctcgtcg atgagaatga tctccacacc gggcgcggcc tgaccgcagg accccggctt
     3361 tctgagctgg tccgcgggca gcatgatggt gttcacgcca agctcggtgg acccgtaaac
     3421 ctcgaacagg ctgtcggccg gaaagtgctc gaggtaggcc tttttcagcg cgaagctcca
     3481 cggagccgcg ttggcgatgg tgatgcgcac gctcgagagg tcatagcgct tgatggtctc
     3541 gggcggcaac tgcgtggcta gccttacggg cgtcggcgcg ctgaaggtga aggagacccc
     3601 atacttgtcg atgagtcgca gccagtcctc ggcgtcgaat ttgcgctgca ggacgatggt
     3661 gttgccgaag gcgaacgcaa acgccatgaa gccgctgggt ccggagtgat agagcggccc
     3721 ggtggtcagg tagacgtcat cgcccctgag ctgcatcagt tccatcagcg cggcaatcag
     3781 ggccggatcg ccggtgtcct tgcgcaccgc gcctttcgga cgaccggtgg tcccggaggt
     3841 gtagatcatg gtgccggagg tcgtcgggtc gactttcact gcgagttcct cggtgccctc
     3901 ttgagccagg cgctcctcgg ccgacgtctg tcccggtcca gccggaccgt cgaatatcag
     3961 gacctcccga acctggggga tgtcgtcccg aatctcggcg aacaggtcgg cgtactcggc
     4021 atctgcgaaa accgtcacgg cgtcggagtt atccaccacg taggcggctt cctctgcggt
     4081 cagccggtag ttcagcggca cggcgactgc gccgatcttg cgcgcggcga tgccggccgc
     4141 gaccacccat gcagaattgg gcccgcacca gatcaaccgg ttgccggctt ccacgccggc
     4201 gttctgaaag acccgtgcga gggcgttcgt gcgctcttcc agctctgagt acgtccactt
     4261 cagcagggtt ccgtcgggcc ggtcgtcgat cagcgctggc ttatccggct ggctgcgcgc
     4321 cagctcggtc aacggatccg tcatctcgct ctcctcgtgc cccgaatgag tttcccgggc
     4381 tgatggtagc cgggcgttcg tggtcgtgca tccgcagcca ttcgtaaccg acgggcagca
     4441 ggcgtagaat tctccgggaa cgaacgaact tcggggagtg tgcttatggc cagcaatccg
     4501 caatctgggg atgtctctga catccagctg ttcgacgtag aaaccctcaa ggacccctgg
     4561 gatgcctatg cccgcctgcg cgatcacgcc cccgttttct tcgtacctgg ttttgatatc
     4621 catgttgtga cgcgatacga cctcgtgcgc gaggtcatca aggacacgga aacctactcg
     4681 agcaagttcg acttgttcct caaccgttcc cgggagctga tgtttgcatc ggcaccgccc
     4741 gaggtgcagg cggagctcat ccgtctcaac gacgagatga tccagatccc acccacgatg
     4801 ctcacgctcg acgagcccga gcatacgaag tatcggtcgc tggtatcgca gctgttcacc
     4861 gtcagtcaga tccgcaaggc cgaggacgcc gttcaggccg tcatcgacga gacgctggaa
     4921 acgttcgttg accgccgctc ggtggacttc atggacgcgt ttgcgtttcc cgtgccgctg
     4981 aggatcatcg ccgatcggct gggtattccc gaggacgacc gagcgttctt cgacgacgcg
     5041 gccaccgccg cggcggccgg tctccggctc agccccatca cgcccgaaga aatggtccgc
     5101 cgggcccagc tgggggtgga tctgcagaag ctgctggtcc ggataatcga agagcgacgg
     5161 accgaccctc tcgaggacat gatcagcatc ctcgcgaaca gcaagctgga ggccgaggat
     5221 cgttacctca ctcatggcga gtgtctgagc atcctcaacc agtttctcgt ggcgggacac
     5281 gaaaccacga cgagcacgtt cggctgggga atgctgctgc tgtgccgcaa ccccgaggcc
     5341 cagcgggcgc tgcgcgccga tccgtcactc acgcggacct tcgtggagga ggccctgcgg
     5401 ctggaagcac ccgtccaggg gctcccgcgc ctggtgacga aggataccga actcggcggc
     5461 gtcgagctgg aggcaggcaa gctgatcatg atccgctacg gtgcggccaa tcgcgacggt
     5521 cgtcagttcg agaatccgga cgagctcgac ctgcaccgca agaaagcggg catgcagctg
     5581 gcgttcggtt ccggggttca tcactgcatc ggcgccccgc tggcccggca ggagctgaat
     5641 ctgggttttc cgaccctgct acggcacatg agcgagattc gtctatcggc cgatcatccg
     5701 cccccggagg cggaggccag cttcattctc cgcaacctgc cggagctgca catcgactac
     5761 gacttgatcg agccctgatc gccgagacgc cggatcatca tgaaagcggc gctcatggaa
     5821 aatggtcgga tctgggtaga caccgtcccc gatcccgagc ctcgactcgg agaggtgctg
     5881 gtcaagtccg cgggttgcgg gatatgcgga tctgacctcc atgcctaccg tcacacggag
     5941 gagttcgtgg cgaccagccg cgagtccggg ggcgctttcc gcctgaccac catgaatccg
     6001 gtggtcctgg gccacgagtt ctgcgctgag gtcgttgact acggacccgg gacgcagcgc
     6061 cgccttcccg ttggcacctt ggtctgtgcc attcccgcgc ttccgcgggg cggcggtttc
     6121 gagcccatcg gttactcgga ggacgcgccg ggggggttcg ccgaatactt tctgctcaac
     6181 gagcgattga tgatgcccgt accccctggg ctatcctggg agcttgccgc gctgacggag
     6241 cccatggccg tcggccacca cgccatcaac aaagcaaggc tcgatggcga agcggttctc
     6301 gtcatcggcg ccggtcccgt aggtcttgca gtgatcgcaa acctcaagca gaggggcgtc
     6361 gggccggtgg tggtcgccga gctctcaccg ggtcggcgag ctctggccga gcgcctgggg
     6421 gccgacgtcg tggtggatcc cgccgcgggc tcgccttacg cggtcccgga ggtctcccgc
     6481 gcgaagggcc tggtgatctt cgagtgcgtg ggcgtgccgg gcatgatcgg gcagatattc
     6541 ctcgacgctc cgcgggaagc caagatcgtg gtggtaggcg tctgccttca gcaggacgtc
     6601 agtcgtcccc tgatcgcaat caacaaggag ctgaacgttc agtacgtgct ggggtacagc
     6661 attgacgagt tcacggaatc cctgaacatg atcgccgacg gcacgctgga cgtggaggcg
     6721 atgatcacgc accggatcgc tctggaggat gtgcctcggg ccttcgacga tctgggcagt
     6781 ccggaagtcc agggcaaagt gctcgttcag ccctgggcct gagctcgatg cctgcgaact
     6841 aggggtaaac cgaggctgtt atcccgacgg gattctagct atcgttgcgg tttttccact
     6901 cggagcacct agagggagac gtatgcccaa gatcaccttc atcacccacg acggcgtcga
     6961 gcacgtcgtc gacgccagca acggggcgtc tgtcatgaat gcagccatcg acaatctcgt
     7021 ccccggcatc gatgcggact gcgggggtga gtgctcctgc gcgacgtgtc acgtctatgt
     7081 cgacccgagc tggacggacg tggtcggcgg ccccggcgat cgcgaggaag aaatgctcga
     7141 tctcaatccc gagcgcgagt ccaactccag actgtcttgt cagatcccgg tccgcgacga
     7201 gctcgaaggg ctggtcgtcc gggtacccga gtttcagatg taaaacggtg gcgtatgagt
     7261 gaagcaatct acgaaaatcc aaacgttcta gagcaggacc cgtggcagct ccccctggag
     7321 gacatcaatc ccgcccaggc tgagctcttc gagaacgact gccactgggc atattttgag
     7381 cggcttcgcc gcgataaccc cgttcattac tgccgggaca gtattttcgg accttactgg
     7441 tccgtgacga agtacgccga catcaagtac gtcgatacga atcacgagat cttttcctcg
     7501 gagcctacga tcgttctgga cgatcaggac gaggactttc cgctgcccat gttcatcgcc
     7561 atggatccgc cgaagcacga tcgccagcga aaagaagttg ccggggttgc ggggccgcga
     7621 aaccttgctg ctctggaacc gaccatccgc caacgcgtgt gctcgatcct cgattccctg
     7681 ccggtacgcg aaaagttcaa ctgggtggac ctggtctcca tcgagctgac gacccagatg
     7741 ctggcaacgt tgtttgattt tccgttcgag gatcgacgca agctgacccg ctggtcggat
     7801 gtggcgactg cccccatcgg ggttctggtg gataccgacg aggcccgcca ggccgagttg
     7861 ctcgaatgcc tggagtattt cacgggattg tggaacgagc gggtcaacca gccgcccggc
     7921 aacgatctgg tctcgatgct cgcccatgga gagtccacca agaacatgga gccgatggag
     7981 tacctcggca acctggtcct gctgatcgtc ggaggcaacg acacgacgcg aaactccatc
     8041 agcggcggcg tgctggctct caatcagaat cctgatcagt acgagaaact gcgcgacgac
     8101 ttgagcctca ttccgaacat gtgctccgag atcattcgct ggcagacgcc gctggcctat
     8161 atgcgtcgag ttgccgttga agatacggac cttggcggcc agcggatcaa ggcgggagac
     8221 aaggtggcca tgtggtacgt gtcgggcaac cgcgacccgg acatgttcga cgacccggat
     8281 gccttcatca tcgatcgggc gaacgcgcgc aagcacatcg cgttcggttt cggcatccat
     8341 cggtgcatgg gcaaccgggt ggcggagatg cagctgcgca ttctctggga agagatactg
     8401 cgccggttcc acaccgtcga gctcgtgggc gagccggtta gggtcaagtc gtcgttcgtc
     8461 aaggggtatc tcgacctgcc ggtgattctc caccccaatt gagagcccgt tccccaggag
     8521 agcacggtcg ccgacttcga aaagttgcca aggattgcag gtgattgagg acgacagcca
     8581 gatgagtgaa cccgacacgt cctgctcgct ggtcgttagc cgtgagctct tcgtgccccg
     8641 cgagctcgtt ttcgacgtct ggacccatgc cgatcacctg gagaactggt actcgccggg
     8701 ccccgatttc gacaggcatg ccgaggtcga cgcccgtgag ggcggcgctt atctgctgac
     8761 ctggaccggg ttggacgggc gcgcttacgg gcaacgaggc cgtttcgata gctttcacgt
     8821 cccggacaag ctcagttaca cctcgatatt cgaagtcgac ggcgaggagc ggatcagcac
     8881 gcggacccac gtcagctttg ccgaccttgg tggcggtacc cggatcgaca tcgtggtcga
     8941 tggctacccg gacggcggct cgagagacgc gcaccaggca agctgggcgc ttttcctcga
     9001 tcagctggag gcgtactttt cagtcatctg aggcccgccg ggatcagctg gtggaggcga
     9061 agaagaccaa aagggtcatg tcctcctcaa tctcgaaaaa tgaatgttcc tccgtcgcct
     9121 gaacgtagag gacggatccc ggccgcacct cgtatatctc gtcgcctacc ttcagccggg
     9181 ctcggccgtc gacgacgaag tacatctcgt cctcgtcgtg aggggtctgc atgtcctgag
     9241 agcctgcggc caggtgatac agaccacaag agaccgaggg cacccgcaga aactctttgt
     9301 actccaccgc gccgcccttg agtttcgact tgagatcgtc gacgtgaaag acctgccagg
     9361 cgctcgtgtt catgtttccc ccgttgaagg cattccggat gctcgaagac ttcagggcaa
     9421 ttgtacaggg tttacggtgc aggttcggct gtgggctcgg gggcctgcaa accgagcagc
     9481 tcgtggagct cgacacgctc cggtccaggc tgcagacgca tggtcgcgtt acgcttgcgc
     9541 ccgcgcagca tgtagcgcag cacggtgcgc cggatacgcc agtcgttcag gaactctccg
     9601 ggcttttcga gcagatggaa cgaacgcaag gcgccgcgca gcacgtgggc ctgctcccgg
     9661 gaagcggcgc tcagcgcgtc gccgaatgcc agcgcgaacc agcggcgcaa ggtccgcggc
     9721 ggttcgagca gcccgcccgc catgctcgcc gttgcgcgtt tgatgccgcg gcgatcctcg
     9781 cccaggctcg cgtcgtaaat gggccttagc tcgctcaccg tgcgttgatg aaaggccagc
     9841 gctcgctcga ccgggtccga tgtctcggcg agcacgtcag taagaatgct ggcatgcagg
     9901 agcccggtgc tgcatccgcg tccgtacaaa gggttcgtgc gcacggcggc gtcgccaatg
     9961 gcgaaaaaat tcacggccag cggttggccg tcgcggacga agtttcgcca tacggcgttg
    10021 atgtcgccga tgccgaatgg atcggtggtg gcctctgcgc ggccatcggc cagccagggt
    10081 tcgaggcctg gaatgctcaa gcagatcctg tcgaacaggt cgccgctgcg gacggccttt
    10141 ctgatggcgg tctcgggcgt gggcacgcac agaatgatag cgaagtggcc gttgtcgcca
    10201 gggaacacgc cgaacttgag gtaccccaga tcgcccgccc cggaacggtc gcttcgggca
    10261 ggctccgatc ggccctcctt caggcggtaa tgccgcgtgt agtagacgag ctccgcatca
    10321 tgtttttcct cggggatggt gcaaccccgt tgtgcgaacc accgcggaaa gcgggttcgc
    10381 cgtccgctcg cgtcgatgac gacgtctgct tgaaacggtg tcacctcgcc ttcgtgctca
    10441 cgctgaatca tgagcccctt aacctgcaga cggccccctt ccgagtctgt aatgaggtcc
    10501 gtgaccttac agggattcac gatcctgatc ccgggaagag cgcccacgta ctggcgaatg
    10561 atggtctcta tggtggctcg gcgacagagc aggacccaga gcttctcgtc acccctcacg
    10621 ggtcggtaac tctctcgcag ctccgccggg agcatctcct gtaaatcgac ccgtctcgct
    10681 ccagcagcgt aaaaggcatc gagaagctca gggtagcgct cctcgatcag gttgcacatc
    10741 aggcccagaa acgcatgcgg gtgtcggaac tgcgcggcgc cccgacgctc ccagctgaaa
    10801 aaagcctcct ctgcgttgcc tgcaggtggc gagacgtcct tgtcgaacac ggtgatgtcg
    10861 tgcccttgcc ccgcgagcgt catggccgtg cacatcccgc agatgcccga accgacgatg
    10921 gcgatgctct ggcccacagg gttgttgcgt cagatcgtgt cagagacgcc gcggacgaac
    10981 agcggttcca tccctagggc gttcacgtcg caacgcatgg cgtccggatc cggcgcctcg
    11041 gcaggctggc tcgcggcctc caggatcagc ggaagtatgg tcgcgtcgct ggaggtgttg
    11101 aggaagaggc cggggcggga gaggacgaag tgcacggctc gctgaaggct ttcccggtcg
    11161 cgcaggggca tgtaccagct gaaacgcttg tccgcgtcgt cgtccgtcca ccgccggcgc
    11221 gccaccgact tgatcgtctg caccgcgacg tttcgggatt tgcagatctt cagcagcgct
    11281 tccacgtctg ccgcgtagtc agattgctgc atcatcgtga agttgtaggg caggagcacg
    11341 gagtcgaagt cgaactgctc gaggcttttc agatgcatcg cggcgacgcg ggtaccgtga
    11401 ccggtgacgc cgatgaagcg gaccaggcct tcggctctgg cgtctatcag tgcctccagg
    11461 gcgcccccgg gggccattgc ggtttcccac tcctgctcgt cgaccagatt gtgcatctga
    11521 atgagatcga cctgatccac gcctaagcgc tccagcgacc tttcgagctg tgcacgggct
    11581 gcggagcccg tccgctggtc ggttttggtg gcgagaaaga agctcgcccg atttgctgcc
    11641 agccagggcg ccagccgcag ctcggaatcg ccgtagcttg ccgccgtgtc gatgtgattg
    11701 accccatgct ccagcagggt ggccaggacg ccctcggcgc gctccgggct catgcttccc
    11761 agcgcggccg cgccgaatag ggtcctggtg ctctcgtggc ccgtgcggcc aaaaggaatt
    11821 ttctcgatca tgtcgatccg ctgcttgctc gagtgactgt aatcatggcc caacggtcca
    11881 ggacggtgca acgagccctc cgggtcaggc gggcaggttg accaccttga ccatgccttc
    11941 gttcagagat ccggaccgga aacctcgcag gtccacggca acgaaccggt agccgcaggc
    12001 gcgaatggcc gactcgatgt ccgccgcacg atcgagcgcg gcgggtatct cgtcgggcag
    12061 cagttcgagc cgcgccacgt ccccgtgatg ccggatacgg aactgcgtaa aaccgaaaga
    12121 cgccagcaca tcctcggcct gttcgacctg tgccaatcgg ttctcgtcga tggccgttcc
    12181 gtagggaaaa cgactcgaaa ggcaggcgga ttggggtttc tgagcattgt ccaatccgag
    12241 ctcgtcggct aaagcccgga tatcggcctt tccgaaaccg gcctcgacca gcggggcggt
    12301 cacgccgtga tcgtctgccg ctttgaggcc cggccggtga tcgcccagat cgtcgcaatt
    12361 cgtcccgctg aagatcatgg cgtaaccctc ttcccgggcg atggccgtca gcgcggcgta
    12421 cagagacgat ttgcagtgga agcagcggtc gatgggattg gcccgataat ccggcttg
//
LOCUS       DBNHLM010000180         4429 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_146208, whole genome shotgun sequence.
ACCESSION   DBNHLM010000180 DBNHLM010000000
VERSION     DBNHLM010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_146208"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="AC16QF_08275"
     CDS             complement(<1..524)
                     /locus_tag="AC16QF_08275"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF014477.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR6448594.1"
                     /translation="MCRLVYFEGVIVRSAPRLIRDGAKRWLVWTLVGLLAGCGDPGYQ
                     RHGGSAMGTGYRVTAKCPGEVGDQVTAVLDAVNADMSTYLPESTLSVFNRGPVGEWLP
                     VSADLIEVVEAAHDLSELSGGAFDVTVGPLVNLWGFGPNGAPAQRPAAARVEFVRAKV
                     GYRYLESRRAPPALR"
     gene            618..1220
                     /locus_tag="AC16QF_08280"
     CDS             618..1220
                     /locus_tag="AC16QF_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6448595.1"
                     /translation="MASILFLRHGQIAANAKGRWNGSTDSELTWRGRRQARRTAKHLA
                     AREAEITAIYSSPLRRTRFTAEAAADRLGLPVTIADGLREYAIGEWEDLPFRHLAREH
                     DFVNTTIRDPDFAPPGGESLRDVADRVVPTILEIHQRHREDSKVLVVGHGAAMAVAFG
                     AILERNPARWTNYHFANCSLSELVISPAPYVNFFNSTQHL"
     gene            1248..2018
                     /locus_tag="AC16QF_08285"
     CDS             1248..2018
                     /locus_tag="AC16QF_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6448596.1"
                     /translation="MYEYCSVEKDDHIMTVTIERPDRLNALHPPANAELGEVFDDFAA
                     NDDMWVAIITGRGRGFSAGNDLRYQAEGGERVPMPRGFGGLTSRFDLNKPVIAAVNGV
                     AMGGGFEIALACDLIIASEKALFALPEPKVGLAALAGGLNRLPRQIGPKRALGMILTG
                     RHVPAEEGLQLGFVNEVVPHDELLAATRRWAEQICACAPLSIRASKDVVYRSLDSASL
                     QASMQAQYDSVKAMVKSEDFIEGPKAFAEKRPPAWKGR"
     gene            complement(2044..2241)
                     /locus_tag="AC16QF_08290"
     CDS             complement(2044..2241)
                     /locus_tag="AC16QF_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448597.1"
                     /translation="MKDPAVRSYPEQFATTPDLLHVVIPRIEIIAEMAVRDERDALND
                     VCLAVACVADAIGSAQLAAQG"
     gene            complement(2491..3222)
                     /locus_tag="AC16QF_08295"
     CDS             complement(2491..3222)
                     /locus_tag="AC16QF_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4336 domain-containing protein"
                     /protein_id="HIR6448598.1"
                     /translation="MRLFDRALSRLHPEQLPVMGARLRSIGRQIWVADRELQMPGGIR
                     LPIRMVVMGDGRGNLLCYSPVELDEGTAEALDGLGKVRWIVAPNRYHRSFTAGCMSRY
                     PQAQVSGFLPAPGDGFAAPAAVPFGGCELTTVSLRTGFAEIVAYHDLSETLVVADLLF
                     NVRNGNRRLQAVMRVNGAWQKAGHTRLQRWLILRDPDGLTKFRRWALSRPFVQISMAH
                     GLIVQEDARECLYRLQRGGPFGAPH"
     gene            complement(3248..4033)
                     /locus_tag="AC16QF_08300"
     CDS             complement(3248..4033)
                     /locus_tag="AC16QF_08300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6448599.1"
                     /translation="MGPQSSVETMTRCAVGAERLGFESLWITDHIAIPPDDAEGSGGR
                     YLDTLTTLAYLGGVTRTIRLGSGVIILPYRPPLPTAKQVATVQELTGGRLLLGVGIGW
                     MDAEFRALGVDRHQRGRISDETLDYLNRCFTEEVMEANGQPFLFRPCPDKPPVYVGGS
                     APHALLRAARYGDGWLPMALEPDPLGEAMVEYRRITEELDRPAGQVTLMRGLPLGKPD
                     ATRALLEQYQGLGLDRLVCALGYDDADAYLHQLEQLAELIDSK"
     gene            4188..>4429
                     /locus_tag="AC16QF_08305"
     CDS             4188..>4429
                     /locus_tag="AC16QF_08305"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HIR6448600.1"
                     /translation="MPQAIYLLAFVGGFVIMSLELLGGRVLAPYFGSSIYVWGSIITV
                     FMLSLSLGYLWGGRLSLSHPSMFRFGIIFMTGAVTL"
BASE COUNT          824 a         1506 c         1357 g          742 t
ORIGIN      
        1 cgcagagccg gcggcgcccg gcgggactcg aggtaacggt aaccgacctt cgcccgcacg
       61 aactccactc gagccgccgc ggggcgctgt gccggcgccc cgttcggccc gaagccccaa
      121 agattgacga gcggccccac cgttacgtcg aacgccccgc ccgacaactc gctcaagtca
      181 tgggccgctt ccaccacctc gatgagatcg gcgctcaccg gcaaccactc gccgaccgga
      241 ccgcggttga acaccgaaag cgtggattcc gggaggtagg tggacatgtc ggcgttgacg
      301 gcatccagca cggcggtgac ctggtcgccc acctcacccg ggcacttggc cgttacccga
      361 tagccggtcc ccatggcact gcccccatgg cgctgataac cggggtcgcc acatcccgcc
      421 aggagaccga ccagcgtcca gaccagccac cgcttggcgc catcacgtat caatcgcggc
      481 gcgctgcgca caatcacacc ctcgaagtag acaagcctgc acaccaggaa ataccatccc
      541 gcccgcatcg cgcccgacac cgggcttcac accgctgcgt ccctgtgggg cgcccggcat
      601 catgacgtaa atcgaacatg gcctcgatcc tctttctacg ccacggccag atagccgcca
      661 acgcgaaggg ccgctggaac ggctccacgg acagcgagct gacgtggcgc gggcgccggc
      721 aggcacgccg caccgcaaag cacctggccg cgcgggaagc agagatcact gccatttact
      781 ccagcccgct gcgccggacg cgattcacgg ccgaggcagc cgccgacagg ctcggtctgc
      841 cggtaaccat cgccgacggg ttgcgggaat acgccatcgg cgagtgggag gatctccctt
      901 ttcgtcacct cgcccgcgag cacgatttcg tcaacacgac catccgcgat cccgacttcg
      961 cgccgcccgg cggtgaatcg ttgcgggacg tagccgaccg cgtcgtgccc accattctgg
     1021 aaatccacca acgccaccgg gaagatagca aagttctcgt cgtcggccac ggcgcggcca
     1081 tggccgttgc ctttggagcg atactcgaac gcaaccccgc gcgctggacc aactaccact
     1141 ttgccaactg cagcctgagc gagctggtga tttcgcccgc gccttacgtt aacttcttca
     1201 actcgaccca gcacctctga ccgacctcaa cgggagaacg aaagcgcatg tacgagtact
     1261 gcagtgtgga aaaagacgat cacatcatga cggtcaccat cgagcgaccc gaccgtctca
     1321 acgcgctgca cccgccggcc aacgccgagc tcggcgaggt attcgatgat ttcgccgcta
     1381 acgacgacat gtgggtggcg atcatcaccg ggcgcggcag agggttcagt gcggggaacg
     1441 acctgcgcta ccaggccgaa ggtggagagc gcgtacccat gccccgggga ttcgggggct
     1501 tgaccagccg tttcgatctc aacaaaccgg tgatcgccgc ggtcaacggc gtggccatgg
     1561 gcggcgggtt cgagatcgcg ctggcctgcg acctgatcat cgcctctgaa aaagccctct
     1621 ttgcactgcc cgaacccaag gtcggtcttg ctgcgcttgc cggcgggttg aaccggttgc
     1681 cgcggcagat cggcccgaag cgcgcgctcg gcatgattct caccggccgt cacgtgcctg
     1741 ccgaggaagg attgcagctc ggcttcgtca acgaggtggt tccacacgat gagctgctgg
     1801 ccgccacgcg ccggtgggcg gagcagatct gcgcctgcgc ccccttgtcg atccgagcca
     1861 gcaaggacgt ggtctatcgc agcctcgact ccgcttcgct gcaggcgtcc atgcaggccc
     1921 agtacgacag cgtcaaggcc atggtcaaaa gcgaggattt catcgagggt ccgaaagcgt
     1981 tcgcggagaa gcggccgccg gcgtggaagg ggcgctaaag ccaggaaaat tccagcggcg
     2041 gcgctatccc tgcgccgcca actgggcgga gccgatggca tccgcgacgc aagctacagc
     2101 caggcagaca tcgttgagcg catcgcgctc gtcccgcact gccatctcgg cgatgatctc
     2161 gattcttgga atgaccacgt gcagcaggtc cggggtggtc gcaaactgct cggggtatga
     2221 cctgactgcc ggatctttca tgctctgcct ctcgtccatc tcccgctaag cggccgtcag
     2281 cacatggcat tcaggtttcg tgccagtctg aaataccttg ataaatcatt gttttatata
     2341 tttttatttc catatatcgc cacgattcga aacgtgccga aggtgacgct tgggagaaga
     2401 aactgaccgg gccgggcccg gacggagcca accggccgct attgcctgca gagtcctggc
     2461 cggccgccga ccccacacgc gatcagcgtt tcagtggggc gctccgaacg ggcctccccg
     2521 ctgcagccgg taaagacact cccgcgcatc ctcctgcacg atgagaccgt gagccatgga
     2581 gatctgcacg aagggcctgg acagcgccca ccgacggaac ttcgtcaggc cgtcgggatc
     2641 gcgcagaatc agccaccgct gaagcctggt gtgtcccgct ttctgccacg ctccgttgac
     2701 gcgcatgacc gcctgcaacc gccgattgcc gttgcgcacg ttgaacagca gatcagcaac
     2761 cacgagggtt tcggagaggt cgtgataggc gacgatctcc gcgaagcccg tacgcaggga
     2821 caccgtggtc agctcgcatc ctccgaaggg gacggcggcc ggggcggcga acccgtcgcc
     2881 gggggccggc aggaaacccg acacctgagc ttgcgggtag cgggacatgc acccggcagt
     2941 gaaggaacga tggtagcggt ttggcgcaac gatccagcgc accttgccaa ggccatccag
     3001 tgcctcggcc gtcccttcat ccagctccac cggggaatag cagagcagat tgcctctgcc
     3061 gtcacccatc accaccatcc gaatggggag cctgattccg cccggcatct gcagctcgcg
     3121 atcggccacc cagatctgcc tcccgatgga ccgaagccgc gcgcccatca ccgggagctg
     3181 ctccgggtgc aggcggctca gcgcccggtc gaagaggcgc acgaacatct gcccgatcaa
     3241 agtgctttca cttggaatcg atcagctcgg caagctgctc cagctggtgg aggtacgcgt
     3301 ccgcatcgtc atagccaagg gcacagacga gccggtccag cccgaggccc tggtactgct
     3361 cgagcagcgc ccgggtcgca tccggttttc caaggggaag ccctcgcatc agggtgacct
     3421 gcccggccgg ccggtcgagc tcctccgtga tgcggcggta ttccaccatc gcctcgccca
     3481 gcggatcagg ctcgagagcc atcggcagcc atccgtcgcc gtagcgtgcc gcgcgtaaca
     3541 gcgcgtgggg agccgacccg cccacgtaga cgggaggttt gtcgggacac ggccggaaga
     3601 ggaagggttg gccgttggcc tccatgacct cttcggtgaa gcaacgattc aggtagtcga
     3661 gcgtctcgtc ggagatgcgg ccgcgctgat gcctgtccac ccccagagcc ctgaactccg
     3721 cgtccatcca gccgataccc accccgagca gcagccggcc accggttagt tcctgcaccg
     3781 tggcgacctg cttggccgtg ggcagaggcg gtcggtaggg aaggatgatg acgccggacc
     3841 cgagacggat ggtacgggtt acgccaccca ggtaggcaag agtggtcagg gtgtccaaat
     3901 agcggccgcc gctcccctcg gcgtcatccg gtgggatggc aatgtgatcg gtgatccaaa
     3961 gcgactcgaa tccgagccgt tcggctccta cggcgcaccg cgtcattgtc tcaaccgaag
     4021 attgcgggcc catgttccgc aacgtcacac cgatctgcac gggcagccct cctttgcgat
     4081 tgccgaagac aagctttgcg cgtcaggccc gagtcttcaa ccggtgcgag ttgtggcggg
     4141 acgggaactt tgtaaactgc accctcccat tgcgcagcct gtgcctgatg ccccaagcca
     4201 tctacctgct cgccttcgtc ggcggcttcg tgatcatgtc cctggaactg ctgggcgggc
     4261 gggtgctcgc accctacttc ggcagcagca tctacgtctg gggcagcatc atcaccgtat
     4321 tcatgctgtc tctgtcgctc ggctacctgt ggggaggccg gttgtccctg agccacccgt
     4381 cgatgtttcg cttcggcatc atcttcatga ccggggcagt cacgctcta
//
LOCUS       DBNHLM010000181        11522 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_864759, whole genome shotgun sequence.
ACCESSION   DBNHLM010000181 DBNHLM010000000
VERSION     DBNHLM010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11522)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11522)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11522
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_864759"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="AC16QF_08310"
     CDS             <1..369
                     /locus_tag="AC16QF_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012722828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448601.1"
                     /translation="HNFIYESVWKDNRRRMNERLSTWEILNKYGQDYKVVFVGDATMA
                     PYEITHAGGSVEHWNEEPGAEWMNRFTEIYDRLVWINPTPQETWEYSSSVGMTQQLVN
                     GQMYPLTIRGLEEGMSFLSK"
     gene            389..4264
                     /gene="hrpA"
                     /locus_tag="AC16QF_08315"
     CDS             389..4264
                     /gene="hrpA"
                     /locus_tag="AC16QF_08315"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HIR6448602.1"
                     /translation="MASRRADTSFDPAAVMRRDVHRLKSLRRSNPSAWQALREQSEAR
                     LLARRGQRLEIAYPPELPISQHADEITRLLADHQVIVVAGETGSGKTTQLPKMCLEAG
                     FGYRGMIGHTQPRRLAARAVAARVAEELGARVGEAVGYAVRFSDQVGEHTLVKLVTDG
                     LLLNEIRTDRFLDAYEVIIVDEAHERSLNVDFLLGYLRRLVDRRHDLRVIITSATIDV
                     ASFSEHFGGAPVVEVGGRGFPVETRYADEEALADRSAEELIVDRIRSIEKTPVAGSAR
                     DILVFQSGEREIFDTAHALRQALDERYEILPLYARLSARDQQRIFKPSGYRRVVIATN
                     VAETSLTVPNIGFVIDPGFARVSRYSFRSKLQRLPVEPISQASADQRLGRCGRVAPGV
                     CYRLYTENDYLSRPPYTDPEIKRTNLAQVVLQMRSLGLGKVAGFPFLDPPDPRAVRDA
                     ERLLHELGALEGGGLSKTGRMMARLPVDPRLARMIIAGHEHRSLSEVLVIVSALAAQD
                     PRERPLERQGSADRAHSEFSDPRSDFLAFLNIWQWYLEARQRLSRARLSRELGSRFIS
                     ATRMREWHALHRQLLLACRELALKPNDQPADYSAVHRALLQGSLSLVGLHEEKGSYLG
                     PRNLRFRIFPGSALARRGPKWLMAGEITETRRVYARCVAGVEARWIEEAAGDLVKRRY
                     SEPRWSVRRGEVIALETVTLYGLPLADGRRVSYRKVDPDACRELFITEALVRGGLEGP
                     FEFLLHNRGLIREIVDEESKGRRRDLLIGDAAQADLYAARLPREVCSVSTLGKFLKQA
                     GPEARERLKFSREELLGVASVELPETDYPGALEIRGVAFPLSYRFAPGEPDDGVSITV
                     PVGMLNSLVGEALEWGVPGFFDVLVEQWLRSLPKTKRRLLAPLPDRLGDIVAVLCAAD
                     RFRHGRLLPALGEVLADNFGLKVASTDWDRGRVDPHLLMNIRIVDGSGNLLAQSRDLG
                     ELRSRFAAEIEERMASSAAAELENTGLTAFPEGGLAESVSLKDRSGQTIAYPALLDAG
                     DSVSVKLFAEARERDRANRDGFARLALIAVAQTTRHLKKGLDEQRRLGLLYAALGGAR
                     QLADELLRAAAWRCFFADRPLPRSEADFEQRIRTNRADLADTFSVMLEVFKTVLEKRF
                     EIVTLCETLTSPAFREAVGDVATQLDELVPADVLSRVPPARLADLPRYLDAARYRLEH
                     LQGRVKRDEENTRLVASFRDRLRRLEADPAATDGEVAELRFAVEELRASLFAEPLAAR
                     WAAGGDRSRVSPKRLDRSFEALERALGLI"
     gene            4425..4706
                     /locus_tag="AC16QF_08320"
     CDS             4425..4706
                     /locus_tag="AC16QF_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HvfA family oxazolone/thioamide-modified RiPP
                     metallophore"
                     /protein_id="HIR6448603.1"
                     /translation="MFNRKMKPIAAVIGTAFVASLAATSVANAASPFAANELDRGYLL
                     LADGHAEGKCGEGKCGEDKDAEGACGEDKDAEGKCGEGKCGEGKCGGAA"
     gene            4730..5572
                     /locus_tag="AC16QF_08325"
     CDS             4730..5572
                     /locus_tag="AC16QF_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HvfB family MNIO-type RiPP peptide maturase"
                     /protein_id="HIR6448604.1"
                     /translation="MSSPVHGAGLGLRRGLLAELASMENASVDFMEIAPENWIGVGGR
                     FGRQFRALCERFPMVCHGLSLSIGGPAPLDIDFLKDIRVFLDEHGIDCYTEHLSYCSD
                     DKGHLYDLMPIPFTAEAVRYVAARVRQVQDVLGRRIGIENVSYYVAPGRELTELEFIN
                     AVLAEADCDLLLDVNNIYVNSINFGYDPVDFLNRIDGARSVYVHVAGHYVEAPDLRVD
                     THGSAVAEPVWDLLSAAYNRWGALPTLLERDFNFPPLGELLHEVSRIRSLQAAVRSGH
                     AANG"
     gene            5556..6335
                     /locus_tag="AC16QF_08330"
     CDS             5556..6335
                     /locus_tag="AC16QF_08330"
                     /inference="COORDINATES: protein motif:HMM:NF050145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HvfC family RiPP maturation protein"
                     /protein_id="HIR6448605.1"
                     /translation="MPRTGENLPEFRARQLDFAAHLRNPDLYPCPEGLDARRMRIYVD
                     LVYNNVESFLANGFPVAKEILGDASWHALIRDFLHRHPSESPYFLEIPQEFLAFVSSC
                     DRSELPDYFLELCHYEWVELALATAEDDPPEAGVDPSGDLVHGRPVVSPLIWPLAYRY
                     PVHRIGPGFRPDAPPESPTHLIVFRRRDDSVGFLEASAATLRLVELLSEGATGASAIE
                     RLCQEMPGLDSQVVHDEGVATMMRLREAGIILGTEIAGASE"
     gene            6328..7062
                     /locus_tag="AC16QF_08335"
     CDS             6328..7062
                     /locus_tag="AC16QF_08335"
                     /inference="COORDINATES: protein motif:HMM:NF016236.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0120010 - intermembrane phospholipid
                     transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HIR6448606.1"
                     /translation="MSDGRARGRARFTTILLCALTPLVTPMSGAAQVNPGEAANRGVY
                     GVNDRADRALLRPVAKGYTKAVPGFLRRAVHNVLLNLGTPLVAVNQLLQGKPNEAFSD
                     SGRFLVNSTVGIAGIFDPATKLGIEEHTEDFGQTLAVWGVPSGPYMVVPFWGPSYLRH
                     AFGMIVEFFASPLRLISPARDRYIAYGVTIIDRRARLIGPDEMIAGDDYLFVREAYQQ
                     RREYLILDGAVEEDPFMMDEAWQEEP"
     gene            7157..8266
                     /locus_tag="AC16QF_08340"
     CDS             7157..8266
                     /locus_tag="AC16QF_08340"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR6448607.1"
                     /translation="MSLVVADPDEEALANLRTQLDRLGLDPIPAADLKFASEICEHSP
                     PDLVISGDLDWAERLADMHRRLPVVLAVDRNPERDVLIRALRCGLADVWTLPAEDDFL
                     RGRVDDILRNNEAAASQAEKRLGQYVATLQRDQRAGRYIQMGMLPPSPMAIDRFRFQH
                     RIVPSLMLSGDFVDYFRLTDRHFVFYIADVSGHGASSAFVTVLLKNFSRRLRREYRPS
                     MLQAPGEILEWFNRELLEQQIDKHVAMILGLGDLEKNVVSLVNAGHFPPAIHVAGQSH
                     AVYVEQRGKPVGLFETVSYPETNVELAEGEALVIFSDGVLDALPDMDLAEKEAALLAA
                     ATRAADVDEIWSSLGVQMPIDAADDMTCLLVGRER"
     gene            8268..8783
                     /locus_tag="AC16QF_08345"
     CDS             8268..8783
                     /locus_tag="AC16QF_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR6448608.1"
                     /translation="MAGRILVGCQDGVYVILFEGDVRLTLYTAADRFLDRMFSDVNFK
                     SVVVDLSGTESIDSTSLGLLAKLSILAKRHFDYVPTLISTRPDITRILLSMGFEDVFH
                     IVEVPLERTSQLGELPELVTTEEDMRERVIEAHRTLMSMNESNRETFQDLVAALEAEE
                     QPVMERRRASR"
     gene            complement(8755..9207)
                     /locus_tag="AC16QF_08350"
     CDS             complement(8755..9207)
                     /locus_tag="AC16QF_08350"
                     /inference="COORDINATES: protein motif:HMM:NF016955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HIR6448609.1"
                     /translation="MLSQLLARLKEPLANSEAETAALLPLAAAVLYLEVAWADHDIAD
                     EEMQLIRRTLSEQFDLTAEEVDVIVSESKEQHDASVGLYRFTRTITEAWDEETRFQLV
                     VRLWGLALSDDELHSFEEHTIRKIAELLYVSHSRFIEAKLIAKRDAAP"
     gene            complement(9227..10213)
                     /gene="dusA"
                     /locus_tag="AC16QF_08355"
     CDS             complement(9227..10213)
                     /gene="dusA"
                     /locus_tag="AC16QF_08355"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HIR6448610.1"
                     /translation="MKTANSPAFRKLCVAPMMAWTDPHCRYLLRLCSPNARLFTEMVT
                     TGALLNGPRRELLAFDPEEHPVAIQLGGCEPADLARAAAIAQTAGFDEINLNLGCPSP
                     RVSKGRFGACLMREPELAAACIGAMRDACRLPVTAKIRLGVDDHDSDALLHDFVARLV
                     EAGCHALYLHARKAYLSGLSPAQNRDIPPLQPERAYGLKRQFAELPIILNGGISREEE
                     AAEHLRHVDGVMIGRAAYHDPMFLSRLDARLSGRAPVPLEAVVTGYLAYMDRQLLRGT
                     RLHDMTRHMLGLFKGVPGARRYRRLLSDSARLKANDLNLVHEAIAATTAQAA"
     gene            10532..11446
                     /gene="lpxC"
                     /locus_tag="AC16QF_08360"
     CDS             10532..11446
                     /gene="lpxC"
                     /locus_tag="AC16QF_08360"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103117 - UDP-3-O-acyl-N-acetylglucosamine
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HIR6448611.1"
                     /translation="MLRQRTIRKAVHAIGTGVHSGETVRMTLRPAGENTGVLFVRADA
                     GNATVRATVDNVCDTTLSTSIGENGTEVSTVEHLMSALWGLGIDNLVVELSGAEVPIM
                     DGSAAPFVYLINSVGVRQQFSRKQFLRVTREITVSDGDATASLTPFQGFEAEYTFVAD
                     HPVFNRYPKRAHFLHNEACYLEEVSRARSFGLVKDLEQAQTINRCLGSSLENAVGIGD
                     FEILNEDGLRYEDEFAKHKLLDAIGDLYLMGRPLLAHFRGYKSGHALNHRLAQALLHN
                     EDAWEIVTLDPDEEPSLALQPVRQPLAG"
BASE COUNT         1990 a         3564 c         3722 g         2246 t
ORIGIN      
        1 cacaacttca tctacgagtc ggtgtggaaa gacaaccgcc ggcggatgaa cgagcgtctg
       61 tcgacgtggg agatcctcaa caagtacggc caagactaca aagtggtctt cgtgggagac
      121 gccaccatgg ctccctacga gattacccat gccggtggta gcgtggagca ctggaacgaa
      181 gagcccggcg cggagtggat gaaccgcttt accgaaattt acgaccggct cgtctggatc
      241 aatcccactc ctcaagaaac ctgggagtat tcctcgtcgg tcggcatgac ccagcagctt
      301 gtgaacgggc agatgtaccc cctgacgatt cggggcctcg aagagggaat gagcttcctt
      361 tccaaatagc ctggccgacc atctcacgat ggcttctcga cgcgccgata cctcgtttga
      421 tcccgctgcc gtcatgcggc gggacgtcca tcgtctcaaa tcactgagaa ggagtaatcc
      481 ttccgcctgg caggcgcttc gtgagcaatc cgaggccagg ctcctcgccc ggcgcggcca
      541 gcggctggag atcgcttacc cgcccgagct tcccatcagt cagcatgccg acgagatcac
      601 gaggctgctg gccgatcatc aggtcatcgt cgtggccggg gagaccgggt cgggaaagac
      661 cactcagctg cccaagatgt gcctcgaggc gggcttcggc tatcgcggga tgatcgggca
      721 cacgcagcca aggcgtctgg cggctcgagc ggtggccgcc cgggtcgcgg aggaactcgg
      781 cgcccgggtc ggtgaggcgg tgggctacgc tgtccggttc agcgatcagg tgggcgagca
      841 cacgctggtg aagctggtga cggacggtct gcttctcaac gagatacgta cggacaggtt
      901 tctcgacgcc tacgaggtga tcatcgtcga cgaggcccac gagcgcagcc tgaacgtgga
      961 ctttctgctc ggctacctcc gcaggctggt cgaccgccgc cacgatctga gggtcatcat
     1021 caccagcgcc actatcgacg tggcctcttt ctctgagcat ttcggcgggg caccggtggt
     1081 ggaggtcggc ggacgagggt ttcctgtcga gacccgttat gcagacgagg aagcgctggc
     1141 ggatcgaagc gccgaggagc tgatcgtcga ccgtatccgg tccatcgaga agacgcccgt
     1201 ggcgggatca gcgcgggaca tcctcgtgtt ccagtccggc gagcgggaga tattcgatac
     1261 tgcccacgcg ctgcgccaag cgctcgatga gcgttacgag atactgcctc tgtacgcgcg
     1321 cctgtcggcc cgggaccagc agcgcatctt caagccctcg ggatatcgac gggtggtgat
     1381 cgccaccaat gtggccgaaa cgtcgttgac ggtgcccaac atcggcttcg tcatcgatcc
     1441 cggctttgcc cgggtgagcc gctactcgtt tcgctccaag ctgcaacggt tgcctgtcga
     1501 gcccatatca caggccagcg cggatcagcg tctgggccgc tgcggccggg tggcgccggg
     1561 cgtgtgctat cggctttata ccgagaatga ctatctgagc cggcctccat ataccgatcc
     1621 ggaaatcaag cgcacgaacc tcgcccaggt cgtgcttcag atgcgctcgt tggggttggg
     1681 taaggtcgcg ggctttccgt ttctcgaccc gcccgacccc cgggcagtcc gtgacgcgga
     1741 gcgtctgctc cacgagctgg gcgcgcttga agggggcggt ctttccaaga ccgggcgcat
     1801 gatggcccgg ctcccggtgg acccgcgcct ggcccggatg atcattgcgg gccatgagca
     1861 ccggtcactg tccgaggttc tggtgatcgt gagcgctctg gccgcccagg atccccgtga
     1921 gcggccgctg gaacgccagg ggtccgccga ccgggcccat tcggagttct cggatccgcg
     1981 ctcggatttt ctcgcctttc tgaacatctg gcagtggtac ctggaagccc ggcagcgtct
     2041 ttctcgcgcc aggctgtctc gcgagctcgg ctcgcgcttc atttccgcca cgcggatgcg
     2101 ggagtggcat gcgctgcatc ggcagctgct gctggcttgc cgtgagcttg cgctcaaacc
     2161 gaacgatcag cccgccgatt actcggccgt gcatcgcgcg ctgctccaag gatccttgag
     2221 cctggtaggt ctgcacgaag agaagggcag ctatctgggg ccgcgtaacc tgcgattccg
     2281 tatcttcccc ggatcggcgc tggccaggcg tgggccgaag tggctgatgg ccggagagat
     2341 caccgagaca cgccgggttt acgcgcgctg cgtggccggc gtcgaggctc gatggatcga
     2401 ggaagctgcc ggcgatctgg tcaagcgccg ctactccgag ccgcgctgga gcgtacgaag
     2461 aggcgaggtg atcgcgcttg agaccgtcac gctgtacggc cttcctttgg cggacgggcg
     2521 tcgcgtgagc tatcggaagg tagacccgga tgcctgtcgt gagctcttca ttaccgaggc
     2581 gctcgtgcgc ggagggctcg aagggccctt cgagttcctt ctccacaatc gtggcctgat
     2641 tcgggagatc gtcgatgagg aatccaaggg ccgccggcgc gatttgctga ttggcgatgc
     2701 ggctcaggcg gatctttacg cggccaggct cccgcgggaa gtgtgctcgg tttcgaccct
     2761 tggaaagttt ctcaagcagg cggggcctga ggcgagggag cgtctcaaat tctcgcggga
     2821 agagttgctc ggcgtggcca gcgtcgagct gcccgagacc gattaccctg gcgcgttgga
     2881 gattcggggc gtggcgttcc ccctgagtta ccgattcgct ccgggagagc cagacgacgg
     2941 cgtctccatc acggttcccg tgggcatgtt gaactcgctc gtcggcgaag cgctcgagtg
     3001 gggcgtgccg ggattcttcg atgtgctggt ggagcagtgg ctgcgatcgc tgcccaagac
     3061 gaagcgccgg cttctggcgc cgctgcccga tcggctcggt gacatcgtcg ccgtgctgtg
     3121 cgctgccgat cgatttcgcc acggccgcct gctcccggcg ctgggggagg tgcttgccga
     3181 caacttcggt ttgaaggtgg cttcgacaga ttgggatcgt ggtcgggtcg atccgcacct
     3241 gctcatgaac atcaggatcg tcgatggcag cggcaacctg ctggcccaga gtcgcgatct
     3301 cggcgagctg cgcagccggt tcgccgcgga aatcgaagag cgcatggcat cctcggcggc
     3361 ggcagagctg gagaacacgg ggctcacggc gtttccggaa ggcgggctgg cggaatccgt
     3421 cagcctgaag gatcgttccg gccagaccat cgcctatcca gccctgctcg acgccggcga
     3481 cagcgtctcg gtaaagctct tcgccgaggc ccgggaacgg gaccgtgcca acagggacgg
     3541 cttcgcgagg ctggccctca ttgccgtggc gcagacgaca aggcacttga agaagggtct
     3601 ggatgagcag cgtcgcctcg ggttgcttta cgcggcgctc ggcggggcca ggcaactggc
     3661 cgacgagttg cttcgtgcag cggcgtggcg ctgcttcttc gccgatcggc cgctgccgag
     3721 atccgaggcc gatttcgagc aacgcatccg gacgaatcgc gccgacctgg ccgacacttt
     3781 ctcggtcatg ctcgaggttt tcaaaacggt tctggagaaa cgcttcgaga tcgtcacgct
     3841 ctgcgaaacc ctaacctcac cggcgttccg cgaggccgtg ggcgatgtgg cgacgcagct
     3901 cgacgagctc gtgcctgccg acgtcctctc ccgggtgccc cctgcacggc ttgctgatct
     3961 tccgcgctat ctcgacgctg cgcgctatcg cctcgagcat ctccagggcc gcgtgaagcg
     4021 cgatgaagag aacacgcggc tcgtcgcgag cttccgcgac cggctgcgtc gcctcgaggc
     4081 ggatcccgcc gccaccgacg gggaagtcgc cgagctgagg tttgccgtgg aggagcttcg
     4141 agcatcgctt ttcgcggaac cgctggcggc gcgatgggcc gctggcgggg atcgttccag
     4201 agtctctccc aagcggttgg acagatcgtt cgaggccctc gagcgcgccc tgggcctgat
     4261 ctgagacgta tgcctgggct gcgcgggcct ttatactcgc tcggccggaa gttgccggaa
     4321 ccgtagggga aagcgccggt cacaagaaaa gagcgttcgt ggtaagggcg ctcggaacca
     4381 taacggaggg gtcttcctag gacccgtgtg aggagaactt gttgatgttc aatcgcaaaa
     4441 tgaagcccat tgcggccgtg ataggaacgg cgttcgtcgc gtctcttgca gccaccagcg
     4501 ttgccaacgc ggccagcccg tttgctgcca atgaactcga ccgcggttac ctgttgctgg
     4561 ccgacggcca cgccgaggga aagtgcggcg aaggcaagtg tggcgaggat aaagatgccg
     4621 agggcgcgtg cggcgaagat aaagacgccg agggtaagtg cggtgaaggc aagtgtggtg
     4681 aaggcaagtg cgggggcgct gcctgagccg acgtcatcgc tgacgtctga tgtctagtcc
     4741 ggtgcatggc gcaggcctgg ggttgcgacg cggtttgctc gcagaactcg cctccatgga
     4801 gaatgcatcc gttgatttca tggagatcgc gccggagaac tggatagggg ttggcggccg
     4861 cttcggccgc cagttccgag cgctttgcga acgtttcccc atggtctgtc atgggctttc
     4921 cctctccatc ggcggccccg ccccgctgga tatcgacttt ctcaaagaca tccgggtgtt
     4981 cctcgacgag catggcattg attgctatac ggagcacttg agctactgca gcgacgacaa
     5041 gggccatctt tacgatctga tgccgatccc gttcaccgcg gaggccgtcc gatacgtcgc
     5101 ggctcgcgtc cgtcaggtgc aggacgtcct cggccggcgc atcggcatcg agaacgtctc
     5161 ctactatgtc gctcctggcc gggaactgac cgagctggag ttcatcaatg cggtgctggc
     5221 cgaggccgat tgcgacctgc tgctggacgt caacaacatc tacgtcaact ccatcaattt
     5281 cggttacgat cccgtggact tcctcaaccg aatcgatggt gcgcggtccg tctacgtgca
     5341 cgtcgccggc cactacgtgg aagcgcccga tcttcgggtc gacactcacg gcagcgccgt
     5401 cgccgagccg gtctgggacc tgctttcggc tgcctacaac cgctggggcg cgctcccgac
     5461 gctgctcgag cgcgacttca actttccgcc cctgggtgaa cttctccatg aggtgtcgag
     5521 aattcgctcg ctgcaggcgg cggtacggtc tggtcatgcc gcgaacgggt gaaaacctcc
     5581 cggaatttcg cgctcggcaa ctcgatttcg ccgcgcatct gcgcaaccct gatctgtatc
     5641 cctgcccgga aggtctcgat gcgcgccgga tgcggatcta cgtggatctg gtctacaaca
     5701 acgtcgagag cttcctggcc aatggctttc ccgtcgccaa ggagattctg ggcgatgctt
     5761 cctggcacgc gctgattcgt gacttccttc accggcatcc cagcgagtca ccatactttc
     5821 tggagatccc gcaggagttt ctcgccttcg tcagttcctg cgaccgttcg gagcttcccg
     5881 actactttct cgagctttgc cattacgagt gggtcgagtt ggctctcgcc acggcggagg
     5941 atgatccgcc ggaagccggc gtagatcctt cgggagatct cgtgcatggt cggccggtcg
     6001 tgtcgccgct gatctggccg cttgcctacc gatatccggt gcatcgcatc ggtcccggat
     6061 ttcgtcctga cgcaccccct gaatcgccca cgcacctgat cgtgttccgc cgccgtgatg
     6121 attccgtggg attcctcgag gcgagcgcag ccacccttag gctggtagag ctgctcagtg
     6181 agggcgccac gggcgcatct gccattgagc ggctgtgtca ggagatgccc ggcttggact
     6241 cacaagtcgt tcacgacgaa ggggttgcaa ccatgatgcg cctgcgtgag gctggcatca
     6301 tactgggcac cgagattgca ggtgcgagtg agtgacggca gagcccgagg gcgagccagg
     6361 tttaccacca tccttctctg cgcgctgaca ccgctggtca cccccatgtc cggcgccgcc
     6421 caggtcaatc cgggcgaagc cgccaaccgc ggggtgtacg gcgtgaacga tcgggccgac
     6481 cgcgcgttgt tgcgtcccgt tgccaagggt tataccaagg ccgttcccgg cttcctgcgc
     6541 cgcgcggtgc acaacgttct gctcaacctg ggaactcccc tggtagccgt caaccagctg
     6601 ctgcagggga agccgaacga ggctttcagc gattcgggcc gcttcctcgt gaacagcacc
     6661 gtcgggatcg ctgggatctt cgaccccgca accaagctcg gtatcgaaga gcatacggag
     6721 gacttcggtc agacgctcgc ggtatggggg gtgccgtcgg ggccttacat ggtggtgccg
     6781 ttctgggggc ccagctacct gcggcacgcc ttcggcatga tcgtggagtt ctttgccagc
     6841 ccgttgcgtc tgatttctcc ggctcgagac cgctacatcg cctatggcgt caccatcatc
     6901 gaccggcgtg cccggctcat tgggcccgac gagatgattg cgggagacga ctatctgttc
     6961 gtccgcgagg cgtaccagca aaggcgtgaa tatctgatcc tggatggtgc cgtggaggaa
     7021 gaccctttca tgatggatga agcatggcag gaggagccgt gaagagctcc cgggttcgct
     7081 gccaagccgc gttgcatttg accaatgata ggagtacggt gcgcgtccca ccgatgcggc
     7141 acccgtttag cttgctatga gcctcgtcgt tgcagaccct gatgaagagg ctctggcgaa
     7201 cttgcgaact cagctggacc gactgggtct cgacccgatt cctgcagccg atctcaagtt
     7261 tgcctctgag atctgcgagc actcgccccc cgatctcgtg atcagcggag acctcgattg
     7321 ggccgagcgg ctcgccgata tgcaccgacg gcttccggtg gttctcgccg tggaccgcaa
     7381 ccccgaaagg gacgtgctca tccgggcgct gcgctgcgga ctggccgacg tgtggacact
     7441 gccggcagag gacgacttcc tccgaggacg ggtggacgac attctccgca acaacgaagc
     7501 ggccgcatct caggcggaga aaaggttggg gcagtacgta gccactttgc agcgtgacca
     7561 gcgggccggc cgctacatcc agatgggaat gctgcccccg tcacccatgg caatcgatcg
     7621 ttttcgattt cagcaccgca tcgttccgtc cctgatgttg agtggtgatt tcgtcgacta
     7681 cttccgcctc accgatcggc acttcgtctt ctacattgcc gacgtctccg ggcacggcgc
     7741 atcgtcggcg ttcgtgaccg tgctgctcaa gaacttctcc cgcaggctcc gtcgtgagta
     7801 tcggcccagc atgctccagg cgcccggcga gatcctcgag tggttcaatc gggagctcct
     7861 ggagcagcag atcgacaagc acgtcgccat gattctcggc ctgggcgacc tggaaaagaa
     7921 cgtggtcagt ctggtcaatg cgggccattt tccacccgcc atccacgtcg ccggacagtc
     7981 gcacgccgtt tatgtcgagc agcggggtaa gcccgttggg ctcttcgaga cggtcagcta
     8041 ccccgagacg aacgtcgagc tggccgaggg cgaggctctg gtgatcttct cggacggggt
     8101 gctcgacgcc ctgccggaca tggacctggc cgagaaggag gccgcgctgc tcgccgcggc
     8161 gacccgcgct gccgacgtcg acgagatctg gtcttcgctg ggagtacaga tgccgataga
     8221 tgcggctgat gacatgacct gtctgctcgt cgggcgggag cgctgaggtg gcgggtcgca
     8281 ttctggttgg ctgccaggat ggtgtgtacg tcatcctctt cgagggagac gtccggctga
     8341 cgttgtacac ggccgccgac cggttcctcg accggatgtt cagcgacgtc aatttcaaat
     8401 ccgtggtggt ggatctgtcg ggtaccgaga gcatcgacag cacctcgctc ggtttgctcg
     8461 ccaagctctc tatcctggcc aagcggcact tcgactacgt cccgacattg atttcgaccc
     8521 gtccggacat cacgcggatc ctgctctcga tgggattcga agatgtcttc catatcgtgg
     8581 aggtccctct ggagcgcacg tcccagttgg gcgagctccc cgagttggtg accactgaag
     8641 aagacatgcg tgagcgagtc atcgaagctc accggacact gatgtcgatg aacgaatcca
     8701 atcgcgagac gtttcaggac cttgtcgccg ctctcgaagc cgaagagcag cccgttatgg
     8761 agcggcgtcg cgcttcgcga tgagcttggc ctcgataaaa cggctatggc tgacatacag
     8821 cagctcggcg atcttgcgga tagtgtgctc ttcgaagctg tgcagctcgt cgtctgacag
     8881 tgccagaccc cagagccgga cgacgagctg aaacctggtt tcctcgtccc acgcctcggt
     8941 gatggtgcgg gtgaatcggt acagccccac gctggcgtcg tgctgctctt tggattcgct
     9001 cacgatcacg tccacctctt cggccgtgag gtcgaactgc tccgagagcg ttcggcggat
     9061 cagctgcatt tcctcatcgg cgatgtcgtg atcggcccag gcgacttcca ggtacaaaac
     9121 ggctgcggcc aggggtagca gcgccgccgt ttcggcttcc gaattcgcca gcggttcctt
     9181 gagccgagcg agcagctggc tgagcatcag gcggtctcca tgggagtcat gctgcctgtg
     9241 ccgtggtcgc cgcgatcgct tcgtggacga ggttcagatc gttcgccttc agccgcgccg
     9301 agtcgctcag cagccggcgg taacgtctgg cgccggggac gcctttgaac aggcccagca
     9361 tgtggcgtgt catgtcgtgc agacgcgtgc ctcgaaggag ttgtcggtcc atgtaggcca
     9421 ggtagccggt gaccacggct tccagaggca ccggagcgcg gcctgacagc ctggcatcca
     9481 gccgcgacag aaacatgggg tcgtgataag cggcccgacc gatcatgact ccgtcgacgt
     9541 gtcgaagatg ctccgcggcc tcttcctcgc gggatatgcc cccgttgaga atgatcggca
     9601 gctcggcgaa ctgcctcttg aggccgtacg cgcgctccgg ctgcagcggg ggaatgtccc
     9661 ggttctgcgc gggcgaaagg ccactcaagt aagccttgcg ggcgtgcagg tacaacgcgt
     9721 ggcaaccggc ctcgacgagc cgcgcaacga aatcatggag cagggcgtcg gagtcgtggt
     9781 cgtctacccc cagccggatc ttcgcggtga cgggcagcct gcaggcgtcg cgcatggccc
     9841 cgatgcaggc ggcggcgagc tcgggttccc gcatcaggca cgcgccgaag cggcccttgc
     9901 tcaccctggg tgaagggcag ccgagattta ggttgatctc gtcgaacccg gcggtctggg
     9961 caatcgcggc agcccgggca agatccgccg gctcgcagcc gccgagctga atggccaccg
    10021 gatgctcctc agggtcaaag gcgagcagct ctcgccgggg cccgttcagc agcgcgccgg
    10081 tggtgaccat ctccgtgaac agccgcgcgt tcgggctgca caggcgcagc aggtaccggc
    10141 aatgcgggtc cgtccacgcc atcatgggcg caacgcacag cttgcggaat gctggcgaat
    10201 tggccgtttt catgggccgc aagcttagcc agcgcgcctt tccaatgcca gcggaaggat
    10261 cgttggcgac ggtctggggg cgctgcctcg cgcattcata tccatgagca gcgagcgccg
    10321 gcgcagcgtg gcgacagctg tctgacaagc ccatcagagc cgattgatcg ggcggtctgt
    10381 aatcctctga aaaacatcat tttttccaga ttggcgcgct ttgacctgct tcatatttct
    10441 ccatatttcc ctccatttta tcttcatttt ttcctccatc gagtgtatag ttccgcccca
    10501 agttgctgaa attgatagaa gaaacccttc aatgctgaga cagagaacga ttcgcaaagc
    10561 cgtacatgcg atcggtactg gtgtccattc gggggaaaca gtccgtatga cgctacgccc
    10621 tgccggggag aatacgggag tcctcttcgt tcgcgccgac gcggggaacg caaccgtgcg
    10681 tgccaccgtc gacaacgtct gcgacacgac cctatcgacg tcgataggcg agaacggtac
    10741 cgaggtgtcc acggtcgagc acctgatgtc ggccctttgg ggactcggca tagacaacct
    10801 cgtcgtcgag ctgtcgggcg ctgaagtgcc gatcatggac ggcagcgcgg caccgttcgt
    10861 ttacctcatc aattccgtgg gcgttcggca gcagttctcg cgaaagcagt tcctgcgggt
    10921 aacacgggag atcacagtca gcgacggtga tgccaccgcg agcctcacgc cgttccaggg
    10981 tttcgaggcc gagtacacgt ttgtggccga ccatccggtg ttcaaccgat accccaagcg
    11041 cgcccacttt ctgcacaacg aagcgtgcta cctcgaggag gtgagccgcg cgcgatcctt
    11101 cggactggtg aaagatctcg agcaggcgca gacgatcaac cgctgccttg gctcgagcct
    11161 cgagaacgcg gttggaatcg gggacttcga aatcctcaac gaggacgggc tgcgatacga
    11221 ggacgagttt gccaagcaca agcttctcga cgccatcggc gacctctacc tgatgggtcg
    11281 accgctactg gcgcacttcc gaggttacaa atccgggcac gctctgaatc atcggctcgc
    11341 tcaggcgctg ctgcacaacg aggacgcctg ggagatcgtc acgctcgacc ccgatgagga
    11401 gccgagcctc gcgctccagc ctgtccgcca gccgctggcc gggtaatact cagaggttcg
    11461 atccagggcc cggttaggct cgcgctgctc gcgttcgccg actgccggcc cgtataattt
    11521 gc
//
LOCUS       DBNHLM010000182         8279 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_744128, whole genome shotgun sequence.
ACCESSION   DBNHLM010000182 DBNHLM010000000
VERSION     DBNHLM010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8279
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_744128"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..740
                     /locus_tag="AC16QF_08365"
     CDS             258..740
                     /locus_tag="AC16QF_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HIR6448612.1"
                     /translation="MPSFDVISEVDLQEVRNAVDQASRELRTRFDFKGVDASFEYADE
                     GILLAAMEEFQLGQMLDILKDKLNRRGVDVRALDPGTIEASGKQKRQAFALKQGIDRD
                     TAKRLVKILKDSRLKVQSQIQGEQVRVTGKKRDDLQQAIAALKDAGLEVPLDFTNFRD
                     "
     gene            766..2019
                     /locus_tag="AC16QF_08370"
     CDS             766..2019
                     /locus_tag="AC16QF_08370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HIR6448613.1"
                     /translation="MKLREVLNRRMITCAFTGFASGLPLFVLVQLVQAWLRDEGVGLT
                     EIGLFALAQVPYTWKFIWSPLAERYPLPFLGRRRGWMLVTQVALVAGFAGFGYVDPVL
                     NIEAVFALALLVGFFSASQDIVIDAYRRELLPTEEELALGNSIHVQVYRIAGFVPGSL
                     GLILADVLPWSAVYWTMAGFMAIGVATTLVVREAVLDPRIPPSLKAAVVEPFREYFGR
                     RGWRYGLVVLLFLFAYKFGDSMATALSTTFYLDLGFSKTEIGVVAKNAGFWPALIGGL
                     LGGLFIVKIGINRGLWVFGVVQLITILGFAYLAQVGNDLVVLAVVISAEYLGVGLGTA
                     AFVAFIARETSPALAATQFALFSAVTALPRTLTAAATGWLVDVVGWVEFFYLCTLAAV
                     PGMVLLVWVAPFTAPSNEPQPPGTR"
     gene            2162..2617
                     /locus_tag="AC16QF_08375"
     CDS             2162..2617
                     /locus_tag="AC16QF_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HIR6448614.1"
                     /translation="MGLWFLVFFLTPILEMYLLIEVGGYIGTLPTIALVMLTAVVGVA
                     LLRIQGVETLTRGVGRLQRGEVPATEMAEGILLAVAGALLITPGFVTDAIGFVLLFPP
                     SRTGIARRMLSRLTMVGPDGRPFSPGEPGPGPTVIEGEFERRPPDDGQP"
     gene            2747..3037
                     /locus_tag="AC16QF_08380"
     CDS             2747..3037
                     /locus_tag="AC16QF_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020799908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR6448615.1"
                     /translation="MNIRPLHDRVVVRRLEEESTSAGGIVLPDSAAEKPSQGEVLAIG
                     SGKLLDNGDVRALDVKVGDRVLFGQYAGSTVKIDGEELLVLSESEIFGVLEG"
     gene            3064..4698
                     /gene="groL"
                     /locus_tag="AC16QF_08385"
     CDS             3064..4698
                     /gene="groL"
                     /locus_tag="AC16QF_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620271.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR6448616.1"
                     /translation="MSAKDVVFGDEARSRMLEGVNVLAEAVKVTLGPKGRNVVLDKSF
                     GAPTVTKDGVSVAKEIELKDRFENMGAQMVKEVASQTSDEAGDGTTTATVLAQSILTE
                     GLKSVAAGMNPMDLKRGIDKAVASAVIELEKMATPCEDSKSIAQVGTISANSDEPIGQ
                     IIAEAMEKVGKEGVITVEEGSGLENELDVVEGMQFDRGYLSPYFINNQDSMAAELEDP
                     YILLCDKKISNIRDMLPILESVAKSGKPLMVIAEDIEGEALATLVVNNMRGIVKVSAA
                     KAPGFGDRRKAMLQDIAILTGGTVISEEVGLTLEGATLDDLGTAKRISSTKENTTIVD
                     GAGAKGDIEGRIKQIRSEIEDTSSDYDREKLQERLAKLAGGVAVIKVGAPTEVEMKEK
                     KARVEDALHSTRAAVEEGVVAGGGVALIRALSAIEGLKGDNEDQNVGIAIALRAMTAP
                     LRQIAANSGDEGAVVLDKVRALTGNEGYNAATGEYGDMLAMGILDPAKVTRTALQAAA
                     SVAGLMITTEAMVAEAPDDSPSPGMPGGMPDMGGMM"
     gene            4858..5529
                     /locus_tag="AC16QF_08390"
     CDS             4858..5529
                     /locus_tag="AC16QF_08390"
                     /inference="COORDINATES: protein motif:HMM:NF017945.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HIR6448617.1"
                     /translation="MDQAFIDFLARWSHVVFGITWIGLLYYFNFVQTEYFKEADPDAR
                     TDAFSKLAPRALWWFRWGAMFTFLTGLIMLGVRGAGVTLSITLGSVLGTLMFLNVWLI
                     IWPNQKIVIASNQQVKAGGEADPAAAGAAPKAAIASRTNTLFSIPMLFFMVSSAHLAT
                     NTGIGFGMIGDASMTGLVVSLLIIAALELNGIFGKQGPMTTVRGVIGSSLVLTAVLWA
                     VAALL"
     gene            5664..6410
                     /locus_tag="AC16QF_08395"
     CDS             5664..6410
                     /locus_tag="AC16QF_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448618.1"
                     /translation="MADRPPSEAISATPDDASGYRRGASGRRRGAVAEGPRMIGMSFI
                     MAVLVAGLVVAGWFIANQHQLLAAEQQALDDAEKRLVVLEDRLRVTDEAMTETGQDTR
                     EQIGFWESEIRKLWAVANERNRKWIKDNQVALGRLQKTLDELKGSNAELAGAVGRHET
                     AFSQQQAIIDQLAGIDLQMQRMVAGQRDLADKINVAQQTVAGLQSGLTGRVSENEQAV
                     AAIDAYRLQLNSRLADIERQLNQLMSSANL"
     gene            complement(6437..7069)
                     /locus_tag="AC16QF_08400"
     CDS             complement(6437..7069)
                     /locus_tag="AC16QF_08400"
                     /inference="COORDINATES: protein motif:HMM:NF027851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HIR6448619.1"
                     /translation="MSYILDALKKSEIENDPAAAASLALTQQRESARNRTIAALVVVA
                     LLANAAVLLWIFRPDPGVPQEPVGPSVAAAQAEPVTVPGQRSEPVTPAPTPPEVSTPG
                     EVATTDVPVINPSPPQPPAREPARRPPARIRTQLADLPRDPKSRFPGLAFSTHVYAED
                     ALLRAVVANGTRLQEGDRLNEVTVEEITEDGVIVAFENYLVEIPVIESWQ"
     gene            complement(7072..>8279)
                     /locus_tag="AC16QF_08405"
     CDS             complement(7072..>8279)
                     /locus_tag="AC16QF_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR6448620.1"
                     /translation="NREDTYAYIVHRLARAGGNPKLFSRGALSRLFRISKGVPRLINV
                     VADRALLGAYVEGRHQVTGRIVSRAAREVLGRRPRYRLWIGVGAASVSAAGVAWAYLT
                     LPEVVDLPTTIPAVSSPAVQAPSEPVPVPETTLAEPVPAAITPPARAPSLTSRADGVD
                     GAGSQAASPSETEPAPVPVPVPVPEIAPEIAASVAVEPDIRRPPMSSFQSQRRAYAAV
                     FDAWGADYRTAPPSMIPCNFAPSAGLQCLGLRGTWSDIEAMNLPVVLELWDSERAPYF
                     AAVTHIHDGNMTLHLGDTVLTVTPKALRDVWFGQYVVLWQTPPQYRGSISRGNTHPTV
                     SWLRVQLEALVSDDLSSGRPEYFDPRLDEAVRDFQGSEGLLTDGIVGPATWIRLAARL
                     DLPAPKLDG"
BASE COUNT         1443 a         2549 c         2773 g         1514 t
ORIGIN      
        1 aatacttcgc gtcatcgacg acgaggattg gaatgttttc ccatgacatg taagttagcg
       61 tttgcttcga cgccttgtcg cttccttgat atgcgaggga cccctcgatg tcaaggaagg
      121 ttcatcgctc gtgcggccat ggtctcagga tggtgatctg gttcaccttt ctgcgccgcg
      181 tggggccggc gcctgcagcg cgaaaccagg cgcctggggc gcgatgctag atgctactag
      241 atgccaaagg aaacgctatg ccaagcttcg acgtgatttc cgaggtggat ctgcaggagg
      301 ttcgcaacgc ggtggatcag gcatcgcggg agcttcgcac ccgattcgat ttcaaaggcg
      361 tcgacgccag cttcgagtat gcggacgagg gcatccttct ggctgccatg gaggagttcc
      421 agctgggtca gatgctggac atcctcaagg acaagctgaa ccgccgcggc gtggacgtcc
      481 gtgctctcga tcccggcacg atcgaggcgt ccggcaagca gaagcggcag gccttcgctc
      541 tcaagcaggg tatcgaccgc gatacggcca agcgcctggt caagatcctg aaagactcca
      601 ggctaaaggt ccagagccag attcagggcg agcaggtgcg cgtgacgggg aagaaacggg
      661 acgatcttca gcaggccatc gccgccctga aggacgcagg cctggaggtg ccgctcgact
      721 tcaccaactt ccgcgactga cgcagcccct gccggacggc ccgccttgaa actccgtgag
      781 gtactcaacc ggcgcatgat cacatgcgcg ttcacgggtt tcgcatcggg cctgccgctg
      841 ttcgttctgg tgcagctggt gcaggcgtgg ctgcgcgacg agggcgtcgg gctgacggag
      901 atcgggctgt tcgcgctcgc tcaggttccg tacacgtgga agttcatctg gtcgccgctg
      961 gcggaacgct acccgctgcc cttcctgggg cgccggcggg gctggatgct ggttacccag
     1021 gtcgcgctgg ttgccggctt tgcggggttc ggctacgtgg atcccgtgct gaacatcgaa
     1081 gccgtgttcg ccctggcgct gctggtcggt tttttcagcg ccagtcagga catcgtcatc
     1141 gacgcctata ggcgcgagct gctgcctacc gaagaagagc ttgcgctcgg caactccatt
     1201 cacgttcagg tataccggat tgccggtttc gtgccgggct ccctcggcct gatcctcgcc
     1261 gacgtgctgc cctggagcgc cgtgtactgg accatggccg gcttcatggc catcggcgtg
     1321 gcgaccactt tagtggtgcg cgaggccgtg ctggacccgc ggataccgcc atcgctgaaa
     1381 gccgccgtgg tggagccttt tcgggaatat ttcggccgcc gcggatggcg ctacgggctg
     1441 gtggttctgc tgttcctgtt tgcctacaag ttcggcgaca gcatggcgac cgccctgtcg
     1501 acgacctttt acctcgatct tggattcagc aagacggaaa tcggcgtggt ggccaagaac
     1561 gccggattct ggcccgcgct catcggcggc ctgctgggcg gtctgttcat cgtcaagatc
     1621 gggatcaacc ggggcttgtg ggtgttcggc gtggtgcagc tgatcaccat cctgggcttc
     1681 gcctatctgg cccaggtggg caatgacctt gtggtgctcg cggtggtgat ctccgccgag
     1741 tacctggggg tagggctcgg gacggccgcc ttcgtggctt tcatcgcccg tgagacttcg
     1801 cccgctctgg cggccaccca gtttgccctg ttcagcgcgg taaccgcgct gccgcgaacg
     1861 ctgaccgccg cggcgaccgg ctggctggtg gatgtggtcg gctgggtcga gttcttctat
     1921 ctgtgcaccc tggcagcggt tccggggatg gtgctgctgg tgtgggtcgc gcccttcacg
     1981 gctcccagca atgagccgca gccacccggg acccgctgaa cgagacccgc tcggccttca
     2041 ggcgtgccat ctggcctgcg ggatcatcgc caccacctgc cacacccgct gcctcggtga
     2101 cggttgacta tccgcgagcg tgccctcaga ttgagccgac ctcgtctggt cacccgccat
     2161 catgggattg tggttcctcg tcttcttcct cacccccatc ctggaaatgt acctgctcat
     2221 cgaggtgggc ggctacatcg ggacgctgcc gaccatcgct ctggtcatgc tgactgcggt
     2281 ggtcggcgtt gcgctgctgc gcattcaggg agtagagacg ctgacccggg gggttggtcg
     2341 gctgcaacgc ggcgaggtgc ctgcgacgga gatggcagaa ggaattcttc tggccgtggc
     2401 gggggccctg ctcatcaccc cgggtttcgt gaccgatgcc atcgggttcg ttctgctctt
     2461 cccgccgagc cgcacgggta tcgcacggcg catgctctcc aggctcacca tggtcgggcc
     2521 cgacggtcgc cctttctcgc ccggagagcc gggtcccggg cccacggtca tcgagggaga
     2581 gttcgagcgg cgcccgcccg acgacggcca gccgtaaaac gtttacttcc caggacttga
     2641 atctctccgg gccgccacta ttatatgcgg cccttggatt agcactctga gcgttagagt
     2701 gctaacgatt acccgatagg gactttaact gggagaaaca caggtaatga acattcgacc
     2761 cttacacgat cgcgttgtcg tgcgccggtt ggaagaggaa agcacctcgg caggcggcat
     2821 cgtgctgccc gactctgcgg cagagaagcc ttctcagggc gaggtgctgg ccataggttc
     2881 cggcaagttg cttgataacg gtgacgtccg tgcgttggac gtcaaggtag gcgaccgagt
     2941 tcttttcggc cagtacgccg gcagcacggt gaagatcgac ggtgaggaac tgcttgtcct
     3001 cagcgagagc gagatcttcg gcgtcctgga aggctaacca gcgagattta tgaggaaaaa
     3061 gcgatgagcg ctaaagacgt agtttttggt gatgaagccc ggagccggat gctcgagggc
     3121 gtgaatgttc tggccgaagc ggtcaaagtc acgctgggcc cgaaggggcg caacgtggtg
     3181 ctggacaagt cgttcggcgc gccgaccgtg accaaggacg gtgtgtccgt ggccaaggaa
     3241 atcgagctga aggacaggtt cgaaaacatg ggcgcgcaga tggtgaagga agtcgccagc
     3301 cagacctccg acgaggccgg tgacggcacc acgacggcaa cggtgctggc ccagtccatc
     3361 ctgacggaag gtctcaagtc cgttgcggcc ggcatgaacc cgatggatct caagcggggt
     3421 atcgacaagg ccgtggccag tgccgtgatc gagctcgaga agatggccac gccgtgcgaa
     3481 gactccaagt ccatcgccca ggtcgggacc atctccgcca acagcgacga gcccattggc
     3541 cagatcatcg ctgaggccat ggagaaggtc ggcaaggaag gcgtcatcac tgtcgaggaa
     3601 ggcagcggcc tggagaacga gctggacgtg gtcgaaggca tgcagttcga ccgcggctac
     3661 ctgtcgcctt acttcatcaa caaccaggat tccatggcag ccgagctgga agacccctac
     3721 atcctgctct gcgacaagaa aatctccaac attcgagaca tgctgccgat cctcgagagc
     3781 gttgcgaagt cgggcaagcc gctgatggtg attgccgaag acatcgaagg tgaggcgctt
     3841 gcgaccctgg tggtcaacaa catgcgcggc atcgtgaagg tttctgcggc caaggcgccc
     3901 gggttcggcg atcgccgcaa ggccatgctg caggacatcg cgattctgac cggcggcacg
     3961 gtgatctccg aggaggttgg cctgacgctg gaaggcgcga cgctggacga cctcgggacc
     4021 gccaagcgca tctcctccac caaggagaac acgaccatcg tcgacggcgc gggcgcgaag
     4081 ggcgatatcg aaggacgcat caagcagatc cgctccgaga tcgaggacac cagctccgac
     4141 tacgatcgcg agaagctgca ggagcgtctg gccaagctgg ccggcggtgt ggcagtgatc
     4201 aaggttggcg cgcccacgga agtggaaatg aaggagaaga aagcccgtgt cgaggacgcg
     4261 cttcactcca cgcgcgccgc cgtcgaggaa ggtgtggttg caggcggagg cgtcgcgctc
     4321 atccgcgctc tgtcagccat cgaaggcctc aagggcgata acgaagacca gaacgtgggc
     4381 attgccattg ccctgcgtgc catgaccgcg ccgctgcgcc agattgccgc caactccggc
     4441 gacgagggtg ccgtggtgct cgacaaggtc cgcgcactca ccggcaacga aggctacaac
     4501 gccgccaccg gtgagtacgg cgacatgctt gccatgggta tcctggatcc ggccaaagtg
     4561 acccggacgg ccctgcaggc tgccgcgtcc gtagccggcc tcatgatcac caccgaggca
     4621 atggtggccg aagcccctga cgacagcccg tctccgggga tgcccggcgg gatgcccgac
     4681 atgggtggca tgatgtaaag ttccgagctg gaacgaaagc ccctccccag gaggggcttt
     4741 tttttgcctg gctccaggga ccggggagcc tcatggtcaa ggtgatttct ggagggggtt
     4801 tcgctatatt cgagctcaga gagacgctaa atccatcgct tttggggaga aaacaaaatg
     4861 gatcaggcct ttatcgactt tctcgcgcgc tggagccacg tcgtgttcgg cattacgtgg
     4921 atcggcttgt tgtactactt caacttcgtc cagacggaat acttcaagga ggcggatcct
     4981 gacgcgcgca cggatgcctt ttccaagctg gcccccaggg cactgtggtg gttccggtgg
     5041 ggcgccatgt tcaccttcct cacgggtctg atcatgctgg gcgttcgcgg cgccggcgtg
     5101 accctctcga ttaccctcgg gtcggttctc ggaacgctca tgttcctcaa cgtctggctg
     5161 atcatctggc ccaaccagaa gatcgtcatc gcctccaacc agcaggtcaa agccgggggc
     5221 gaggcagacc cggcggccgc gggggcggcg cccaaggcgg cgatagcatc ccggaccaac
     5281 acgctgttct cgatccccat gctcttcttc atggtttcga gcgcgcacct cgccaccaac
     5341 accggcatcg gcttcgggat gatcggtgat gcgagcatga ccggtcttgt ggtttccctg
     5401 ctcatcatcg ccgcgctgga gctcaacggg atcttcggca agcagggtcc gatgaccacg
     5461 gttcgcggcg tgatcggctc gagcctggtg ctgaccgccg ttctctgggc ggtggccgca
     5521 ctgttgtagg cggcccgacg gcccgtcggg tgaaagatcg gagagcctgc acttgcaggc
     5581 tctttttttt gctgtaactt ccgtgccctg aaccgccgtc cggcctggcc gattggcctc
     5641 agcgtatctg gagacacacc aacatggcag acagaccccc atcggaggcc atatcagcga
     5701 ccccggacga tgcatccgga taccgtcgcg gcgcttcggg aaggcggcgg ggtgcggtgg
     5761 ccgaaggccc caggatgatc ggcatgagct tcatcatggc ggttctggtg gccggcctcg
     5821 tcgtggcggg atggttcatc gccaaccagc atcagctgct cgccgccgag cagcaggccc
     5881 tcgatgacgc agagaaacgg ctcgtggtct tagaagaccg tctcagggtg accgacgaag
     5941 cgatgacgga aaccggccag gatacccggg agcagatcgg tttctgggaa tctgagatac
     6001 gtaagctctg ggccgtagcc aacgagcgca accgcaagtg gatcaaggac aaccaggtcg
     6061 cgctcgggcg cctgcagaaa acgctcgatg agctcaaagg gtcgaacgcc gagcttgccg
     6121 gtgcggttgg gcgccacgag acggccttct ctcagcaaca ggccatcatc gaccaactcg
     6181 ccggcatcga tctccagatg cagcgcatgg tcgccggaca gcgagacctg gcggacaaga
     6241 tcaatgttgc gcagcagacg gtggccggcc tgcagtcggg gcttaccggc cgggtcagcg
     6301 agaacgagca ggcggtcgcc gccatcgacg cgtatcggct gcagctgaat tcccggctcg
     6361 ccgacataga gcgccagctc aaccagctca tgtcctcggc aaacctgtga cgttctgaga
     6421 gagcctttcg ccgcccctac tgccaggact cgatgacggg gatctccacg aggtagtttt
     6481 cgaacgccac gatgacgccg tcctcggtga tctcctccac cgtcacctcg tttagccggt
     6541 cgccttcctg caggcgggtg ccgttggcga ccacggcacg cagaagggca tcctcggcgt
     6601 agacgtgggt cgagaacgcc agtcccggaa agcggctctt cggatctctc ggcaggtcgg
     6661 ccagctgcgt cctgattctt gcgggtggcc gccgtgcggg ctcccgggcg ggcggctggg
     6721 gtggcgacgg gttgatgacg ggaacgtcgg tggtagcaac ctcgccgggg gtgctgacct
     6781 cgggtggggt gggcgcgggc gttaccggct cgctgcgttg ccccggcacg gtcacgggct
     6841 ccgcctgggc ggcggccacc gatggaccga ccggctcttg cgggacccct gggtcgggtc
     6901 gaaagatcca cagcagcacg gctgcgttgg ccagcagggc aactacgacg agggccgcga
     6961 tggttcggtt gcgcgcggat tcccgctgct gggtgagcgc caggctcgcc gctgctgccg
     7021 ggtcgttctc gatctcggat tttttcagcg catcgagaat gtaagacact gtcagccgtc
     7081 cagcttcgga gccggaaggt ccaggcgcgc ggccaggcgg atccacgtgg caggcccgac
     7141 gatgccgtcg gtcagcaggc cctcggaacc ctgaaagtcg cgcaccgctt cgtcaagccg
     7201 ggggtcgaag tattcgggtc gcccgctcga cagatcgtcg cttaccaggg cctccagctg
     7261 aaccctgagc cagctgacag ttgggtgggt attgcctcgg ctgatgctgc cccggtactg
     7321 aggcggcgtc tgccagagca ccacgtactg gccgaaccag acatcgcgca gcgctttggg
     7381 cgtcaccgtg aggacggtgt cccccaggtg cagggtcatg ttgccgtcgt gtatgtgcgt
     7441 taccgcagcg aagtagggtg ccctctcgga atcccacaac tccagcacca cgggcaggtt
     7501 catggcctcg atatcgctcc aggtgccgcg caggcccagg cattgcaggc ccgctgacgg
     7561 cgcgaagttg catgggatca tgcttggcgg ggctgtgcgg taatcggcgc cccaggcgtc
     7621 gaacacggcc gcgtaggccc tgcgctggga ttgaaagctg ctcatcggcg gccgccggat
     7681 atcgggctcg acggctacgc tggccgcgat ctccggcgcg atctccggca cgggcaccgg
     7741 cacgggcacc ggcgcgggct cggtctcgct gggcgacgcg gcttgggagc cggcgccatc
     7801 gacgccatca gcccgcgacg tcaggcttgg ggcccgtgct ggtggcgtaa tcgctgccgg
     7861 tacgggctcg gcaagggtgg tttcgggcac cggcaccggc tcgctcgggg cctgcacggc
     7921 cggtgacgag acggccggaa tcgtcgtcgg cagatcgaca acctcgggca gggtgaggta
     7981 ggcccaggcg acgccggcgg ccgagaccga ggcggcaccg acgccgatcc agaggcgata
     8041 gcgcggccgg cggccgagca cctccctggc cgcgcggctg acgattcgtc cggtgacctg
     8101 gtgtctgccc tccacgtagg cgccgagcag cgcccggtcg gccaccacgt tgatcagcct
     8161 gggaacgccc ttcgagatcc tgaacaggcg tgaaagcgcg ccccgggaaa acagcttcgg
     8221 gtttccgccc gcgcgggcca ggcggtgcac tatgtacgcg taggtgtcct cccggttga
//
LOCUS       DBNHLM010000183         3689 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_385722, whole genome shotgun sequence.
ACCESSION   DBNHLM010000183 DBNHLM010000000
VERSION     DBNHLM010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_385722"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="AC16QF_08410"
     CDS             complement(<1..489)
                     /locus_tag="AC16QF_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012946.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6448621.1"
                     /translation="MTEYDVIIIGAGMSGMYQLHRLRQLGLSVRVYEAGSGVGGTWYW
                     NRYPGARFDSESYSYGYSFSQELLDEWDWSEHFSAQPETLRYLNHVADKFDLRRDMQF
                     DSRVVRATYDDAAPGWEIELESGERARAWLLISAVGPLSAPVMPDVPGIDDFRGEAYH
                     TGL"
     gene            complement(497..1609)
                     /locus_tag="AC16QF_08415"
     CDS             complement(497..1609)
                     /locus_tag="AC16QF_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR6448622.1"
                     /translation="MAGINRAALFFEPGKPFEIVELPIPEPAAGGLTLKVTRANICGS
                     ELHMWRGDGRFAQVITPKGRILGHEATGVVHALGEGVTTDWAGNALAEGDRIAYQYFQ
                     PCGRCRNCTRGMSEACRDSFSIQRGSLEEWPYTRGSFSDYLYLHPRQAIFKVPDHVTD
                     TMVVSANCALAQVVMGLERVNVQLGDRVVVQGCGGLGVYACAIAKERGAELVIAIDGI
                     DERLELAQRMGADHVIDLRQVEDSKARVGAVKDLTGGLGADVVVEVVGFASVINEGLR
                     MLAWGGRYLEMGTFYTGTSFECDPGRLVGQNQRIEAVASYDAASLQRAIEFLARNGDR
                     LPLDDVVVDYPLDQIERAFADQDSGLVRRASLVMGD"
     gene            1714..2436
                     /locus_tag="AC16QF_08420"
     CDS             1714..2436
                     /locus_tag="AC16QF_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448623.1"
                     /translation="MKALKYVLLVVLVAVIGAVAYVVVNAGSIVKQVIETVGSDVLGV
                     PVTVRSVDLSLDGGSIGGLQIGNPEGFETPYALRIEKAAVKINAQASTAKLIVLDQIL
                     VDQADVNAEFVGRNGNLQTLQKNIEASVGPGEPETEASDVKVIIESFDFTNANTRLSS
                     DVLGGGQISVPDLHLTGIGRKESGVTAAEAAKQLFGPVIGAIVKAAVAQQTGIDQDKI
                     KGTISDKIGSGLKSLTGSKSDD"
     gene            2510..3391
                     /locus_tag="AC16QF_08425"
     CDS             2510..3391
                     /locus_tag="AC16QF_08425"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6448624.1"
                     /translation="MVQLTTLPPTATTEEIMEVIHADGAVIVKDMLTGDEVGAVVDEV
                     RPYMDTTPVGADEFAGFKTTRTGALVARSPKCRDLIMNQTVLDATKAFLAPYCLRFQL
                     HLGQIIRLMPGQPAQQIHRDRWAWGKYLKDVEPQFNTIWALSDFTQANGATQVVPGSI
                     DWPDDRVAKPDEICQAVMSAGSALLYTGSVFHGGGENRSDGDRIGMNLTYSLGWLRQE
                     ENQYLSCPPEIARELPEEMQNLLGYQLGSYALGYYTPPLPPGEGPEAVGPQWAVGRRD
                     SHEALGDAELLEAVINM"
     gene            complement(3477..>3689)
                     /locus_tag="AC16QF_08430"
     CDS             complement(3477..>3689)
                     /locus_tag="AC16QF_08430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6448625.1"
                     /translation="PGRVHVYEEWTSSETLEAHLQSHWYRDMGAHLRQYPRKPPTRVI
                     KKYRVDLEEPVYDSEGVARGHFFTES"
BASE COUNT          673 a         1256 c         1174 g          586 t
ORIGIN      
        1 cagccccgtg tgataggcct cgccgcggaa atcgtcgatg ccgggaacat cgggcatcac
       61 cggcgccgac agcgggccaa cggcgctgat gaggagccag gctctcgccc gctcgccgct
      121 ctcgagctcg atctcccagc ccggcgcagc gtcgtcgtag gtggcccgca cgacccggct
      181 gtcgaactgc atgtcacgcc gcaggtcgaa cttgtccgca acgtgattca ggtagcgcag
      241 ggtctcgggc tgggccgaga agtgctcgct ccagtcccac tcgtccagga gctcctggga
      301 gaacgagtac ccgtagctgt agctctccga gtcgaacctc gcacccggat agcggttcca
      361 gtaccaggtg ccgccgacgc cgctgccggc ctcgtacacg cggaccgaaa gacccagctg
      421 ccggaggcga tgaagctggt acatcccgga catgccggcg ccgatgatga tgacgtcgta
      481 ctcggtcatc gtcccttcaa tcccccatca cgagggacgc gcgccggacg agtcctgagt
      541 cctggtccgc aaatgcccgc tcgatctgat ccagcgggta gtccaccacc acgtcgtcca
      601 gggggagtcg gtccccgttg cgggcgagga actcgatggc gcgctgcagg cttgccgcgt
      661 cgtaggaggc cactgcctcg atgcgctggt tctgcccgac gagcctgccg gggtcgcact
      721 cgaagctcgt gcctgtgtag aacgtgccca tttccaggta acggccgccc caggcgagca
      781 tcctcaagcc ttcgttgatc acgctggcaa accccaccac ctcgacgacc acgtccgcgc
      841 cgagcccgcc ggtgaggtcc ttgacggcgc ccaccctggc tttggagtcc tccacctgac
      901 gcaggtcgat gacgtgatcc gcgcccatgc gctgagcgag ctccagacgc tcgtcgatgc
      961 cgtcgatggc gatcacgagc tccgcgcccc gctccttggc gatcgcgcag gcataaacgc
     1021 ccagcccccc gcagccctga accaccaccc ggtcgcccag ctgcacgttg acacgttcca
     1081 gacccatgac cacctgggcc agcgcgcagt tcgcactcac caccatggtg tcggtgacgt
     1141 ggtccggcac cttgaagatg gcctggcgcg gatgcaggta caggtagtcg gaaaacgacc
     1201 cgcgggtgta aggccactcc tccaggctgc cgcgctggat gctgaagctg tcccggcagg
     1261 cctcgctcat gccccgggtg cagttgcggc agcgaccgca gggctgaaag tactgatagg
     1321 cgatccggtc cccttctgcg agcgcgttgc ccgcccagtc ggtcgtgacc ccctcgccaa
     1381 gggcgtgaac cacaccggtg gcttcgtggc ccagaatgcg ccccttaggc gtgatgacct
     1441 gggcgaagcg gccgtcaccc cgccacatgt gcagctcgga tccgcagatg ttggcccggg
     1501 ttaccttgag cgtcagcccc ccggccgccg gctcgggaat cggcagctcg acgatctcga
     1561 acggtttgcc cggctcgaaa aagagcgccg ccctgttgat gcccgccatg tctacctccc
     1621 cagtctgaaa accttgaaac cgccagcgtt taaaggcaac gtgcccccag aaacgcgccc
     1681 tcctggcgca tcctagagca aaggggagga tcgatgaagg cattgaagta cgtcctgctg
     1741 gtcgtgctgg ttgcagtgat cggcgccgtc gcctacgtgg tggtcaacgc gggaagcatc
     1801 gtcaagcagg tcatcgagac ggtggggtcg gacgtgctcg gcgttccggt caccgtccgg
     1861 tccgtcgacc tctccctgga cggcggcagc atcggcggct tgcagatcgg caaccccgag
     1921 gggttcgaaa ccccctacgc cctcaggatc gagaaggcgg cggtgaagat caacgcccag
     1981 gccagcaccg ccaagctcat cgtcctggat cagattctcg tggaccaggc cgacgtcaac
     2041 gccgagttcg tcggcaggaa cggcaacctg cagaccctgc agaagaacat cgaagcctcc
     2101 gtgggccccg gcgagccgga gacggaagca tccgacgtca aggtgatcat cgaaagcttt
     2161 gacttcacaa atgccaacac gcgcctttcc tccgacgtgc tgggaggcgg gcagatctcc
     2221 gtccccgacc tgcacctgac cgggatcggc cgcaaggaga gcggcgtcac cgcggcggag
     2281 gccgccaagc agctgttcgg cccggtgatc ggcgccatcg tgaaggcagc cgtcgctcag
     2341 cagacgggca tcgatcagga caagatcaag ggcaccatct cggacaagat cggctctggc
     2401 ctgaaaagcc tgacggggag caagtccgac gactgagccc ggcacggctt gccggccgga
     2461 cggtcttggg attcaatcat cccgctgaag cagagagagg cgcgacgtca tggttcaact
     2521 caccaccctg ccccccacgg cgaccaccga ggagatcatg gaggtcattc acgccgacgg
     2581 cgcggtgatc gtcaaggaca tgctcacggg cgacgaggtc ggcgcggtgg tcgacgaggt
     2641 gcggccgtac atggacacga ccccggtcgg agcggacgag tttgccggct tcaagactac
     2701 gcgcactggc gcgctggtcg cccgatcccc gaaatgccgg gatctgatca tgaaccagac
     2761 cgtgctggac gccaccaagg ccttcctggc accttactgc ctgcggttcc agcttcacct
     2821 ggggcagatc atccgcctca tgcccgggca gccggcccag cagattcacc gtgaccgctg
     2881 ggcctggggc aagtacctca aggacgtgga gccccagttc aacaccatct gggcgctgtc
     2941 cgatttcacc caagccaacg gcgcaaccca ggtggtgccg ggcagcatcg actggccgga
     3001 cgatcgggtg gcgaaacccg atgagatctg tcaggccgtc atgagcgccg gatcggccct
     3061 gctctacacg ggatcggtct tccacggcgg cggcgagaac cgctccgacg gggatcgcat
     3121 cggcatgaac ctgacctact ccctggggtg gctgcgacag gaggagaatc agtatctctc
     3181 ctgcccaccc gagatcgccc gggaactgcc cgaggagatg cagaacctgc tgggctacca
     3241 gctcggcagc tacgccctgg ggtactacac cccgccgctg cctcccggtg aagggcccga
     3301 ggccgttggc ccgcagtggg ccgtggggcg gcgcgacagc cacgaggccc tgggcgacgc
     3361 ggagctgctg gaagcggtca tcaacatgta gcgtcggccg ccagtgacgg cggtgcgctg
     3421 ggaaaaacgg gcgcacgccc tcccgatcag gtctggaacc agatcaattc ccgatcctag
     3481 ctttcggtga agaagtgccc gcgcgcgacg ccttcggaat cgtagacggg ctcttccagg
     3541 tcgacgcggt atttcttgat gacccgggtg ggcggcttgc gcggatactg gcgcagatga
     3601 gcccccatat cacgatacca gtgactctgc agatgggcct ccagcgtctc agaggacgtc
     3661 cactcctcgt agacgtgcac gcgcccggg
//
LOCUS       DBNHLM010000184        11827 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1044128, whole genome shotgun sequence.
ACCESSION   DBNHLM010000184 DBNHLM010000000
VERSION     DBNHLM010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11827
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1044128"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="AC16QF_08435"
     CDS             <1..389
                     /locus_tag="AC16QF_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448626.1"
                     /translation="VVGVGTQAAALESALRAAEPVSESALQNLAEALAESSTAIEQLV
                     ALQSAAEGGGEESGGNSLELLDRLEKEIGENDPAASDTAEELLKSLEADSAAVTAATA
                     ARDALELFDFAGAGEQLDQIRAGLGS"
     gene            complement(441..1235)
                     /locus_tag="AC16QF_08440"
     CDS             complement(441..1235)
                     /locus_tag="AC16QF_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR6448627.1"
                     /translation="MTDLTTYYRDHWLDVEDERMARYEAMFAWREGQEVLIAPAAIGA
                     GQLVVDYGCGPGGLAVELARRVGSTGKVIGLDINAEFLERARARAADEGVAERVETRL
                     VTGDRFPMDTHAVDRVICKNVLEYVPDPQATINEFHRILRPGGIAHVSDSDWGAVVFE
                     PAREPFARIMSAAAVAFRTPLIGRSLYGMFRRAGFDDVRVQVLAAADTTGALRGVLHN
                     MAGYALTSGQVDRADVDAFISDIDRALDDKTYLAVLPQFLVTGRVP"
     gene            complement(1275..2486)
                     /locus_tag="AC16QF_08445"
     CDS             complement(1275..2486)
                     /locus_tag="AC16QF_08445"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6448628.1"
                     /translation="MATLDILPTQIDFSALHARMQWYVDERIIPHCVCLVMRGTDVLD
                     YRTLGYMDLESREPLREDAIFRMYSNTKIVTSVAAMMLFEEKRFELDDPVARFIPSFS
                     DSQVLRPGAKSAKDVEPARSPITMRQILSHSAGLSYGVFEPTSVVDQLYAQAGLHLLS
                     DTDGTLENLCEVVSRLPLAFQPGTNWRYSVSTDVTARVIEVISGQRFDVFLKERILDP
                     LGMADTDFHVPAEKQHRFTTMYAPVDLFAPMKGGLNKADDPLSGMYSRPHAMLAGGHG
                     LVSTVADYVTFLRALINGGEWHGVRLLQAETLELMRTNQLADGVSVHFPMWEMKDNVF
                     GLGFALKQAPADGEPETAVGEYHWGGIAGTHSWMAPRAGIAGVCMTQRMPGFWHPFSQ
                     EFKRMVYEQLG"
     gene            2737..3447
                     /locus_tag="AC16QF_08450"
     CDS             2737..3447
                     /locus_tag="AC16QF_08450"
                     /inference="COORDINATES: protein motif:HMM:NF016300.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HIR6448629.1"
                     /translation="MTDSRGNFIPGLLVAIGLAVAGYLIGQGFVHARVLDRSVEVKGL
                     AEREVPADTAIWPVQIVAAGDDLQALLVQIEGQSGRVVGFLADLGFTPGEVSVGAPNV
                     LDRQAQNFGDPNTRFRYSVAQTITVFTEAVATVREAGGRLLELGKEGIVVTQNNYDLR
                     TQYLFNGLNDIKPEMIEEATRNARRVAEKFAGDSDSVLGKIRSARQGQFSIQDRDSNN
                     PHIKRVRVVSTLEYYLVD"
     gene            3501..4184
                     /gene="nthB"
                     /locus_tag="AC16QF_08455"
     CDS             3501..4184
                     /gene="nthB"
                     /locus_tag="AC16QF_08455"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003410914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HIR6448630.1"
                     /translation="MNGIHDLGGMTCFGPIVREDDEPVFHSDWERRVFAMNMLSMGQF
                     GSLDQARHAIERMDPAAYLSTSYYEHWLAGIEILGRDSGLLMDAEIASGRSEHTSTPS
                     RPPLDPEFVDAMVRSGSPSTRPVSRRPTFDVGDSVRARNLDVRGHTRLPRYVRGKRGV
                     VSAVHGTHVFPDTSAHGLGENPQPLYNVRFEADELWGDNLARRDCVHIDLWEDYLEAS
                     PVEGRSHEQ"
     gene            4174..4833
                     /gene="nthA"
                     /locus_tag="AC16QF_08460"
     CDS             4174..4833
                     /gene="nthA"
                     /locus_tag="AC16QF_08460"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008006381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HIR6448631.1"
                     /translation="MSNDHDNDHDHDHGGPPNPYHAASRSDDENRALEERVGRIESLL
                     LEKGLVRQDALDRIADIYENDLGPMNGAKVVARAWVDAAFKQRLLEDASAAIGELGYG
                     GLQGEHMVVVENTPEVHNVVVCTLCSCYPWPVLGLPPAWYKSPAYRSRVVIDPRSVLE
                     EFGTAVDDDVEVRVWDSSAEIRYLVLPERPTGTETLTEDQLAELVERDAMIGVTRVAA
                     P"
     gene            4830..5126
                     /locus_tag="AC16QF_08465"
     CDS             4830..5126
                     /locus_tag="AC16QF_08465"
                     /inference="COORDINATES: protein motif:HMM:NF045268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HIR6448632.1"
                     /translation="MTGEREAAPLIAEMPAPAALPRKSGEMVFHDEWERRAFAMAVSL
                     AEQGAFEWSEFQQKLIAAISDAERDDPQQPSRGYYESWLVALEALLQEKELLPG"
     gene            5316..5834
                     /locus_tag="AC16QF_08470"
     CDS             5316..5834
                     /locus_tag="AC16QF_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6448633.1"
                     /translation="MHGIQVEGLMMPSGFRSHRAQVAVCTDLADLRAFISDTSRFHEW
                     IPDLAEVRLLEQSDDGVVYYMRSKAPWPVKDRDMVYRLFGVPESGDAASVALAIEGLP
                     GRLPEVDGAYRMHDVSGQWRIWERGDRLDVQQQIYLNPGDVPRMFANRSMSASLARTL
                     GNLASRYPCPKS"
     gene            complement(5858..6964)
                     /locus_tag="AC16QF_08475"
     CDS             complement(5858..6964)
                     /locus_tag="AC16QF_08475"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6448634.1"
                     /translation="MTDEPIAPVTVYGSSISYFTGKLENYLRIKGIPYELKPLGPGSV
                     RKLIKSRTGSSQMPAVRLEDGRWLTDTTPVIGWFEREYPEPAVIPHDPVQRFFSLLLE
                     DYADEWLWRPAMHYRWFYDVGARFASAHLAQELTRGVYLPMFVKRRAITRRQRNGYTR
                     GDGITADQVPGVEAIYSRTLGQLETIFRSRPFLLGASPSLADVGFSGPFFRHFGLDPV
                     PAEIMRRDAPAVYEWIARLWNAGPVDSSMAIPAGIPDDWAPILDEIGTHYLPYLCANV
                     DAVAAGLRRFDAEVGGVRYHRARWSPYRVWCLKKLRETFAGLTEDEQAEARTLLERHG
                     CWEPMWRHASLPLKDGFCERLPFHADAKMIDVYQ"
     gene            complement(6954..7688)
                     /locus_tag="AC16QF_08480"
     CDS             complement(6954..7688)
                     /locus_tag="AC16QF_08480"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR6448635.1"
                     /translation="MQMWRFLAPSALGFTSRNMWHALIDRATGAPPRALRAMEHVAAH
                     AERHNPQDVLRKLDEFATSVRWMMSIGPEKDKVIAEARAALDRDPRVLELGAYAGYSS
                     IYMADVLGPGTNITSVEVNADNVRAARGNIAHAGLDGRVEVVHGSSSDVIPTLEGSFD
                     LVFLDHWKDLYLQDLQLIETQGLIAPGSVVVADNVGELFGADAYLDYVRHCGRYDSAN
                     RPATIEYTSLPDAVEISVYRGQGDDG"
     gene            7820..8158
                     /locus_tag="AC16QF_08485"
     CDS             7820..8158
                     /locus_tag="AC16QF_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448636.1"
                     /translation="MDEIRSYHYDPEKFEPYRKWALEEAIPFLKDNLNIVGFWLDNGK
                     APEVGGTSPMDMPLGSANVTWVIRWDSEEDREAGHARIFGGQEWQDIWSRHPDANGYL
                     QMEARFADPV"
     gene            complement(8164..10203)
                     /locus_tag="AC16QF_08490"
     CDS             complement(8164..10203)
                     /locus_tag="AC16QF_08490"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HIR6448637.1"
                     /translation="MNDRSRPRSPIAGPALFVTVLAAASAVAEVEWRSYGNDHTEQRF
                     SPIDQVNRSNVGELGLDWALSVPDAVSLNSTPLMIDGVLYFSADRAIVHAVDARSGKL
                     IWSYDPQSWKHAPRGIGVSFNTNRGIAHSEGSIFVGTADGRLVSLDARTGRVKWATRT
                     FGREERKAITGAPRAFDGKVFIGNGGAEFGTRGFVSAYDAADGELLWRFYTVPGDPSL
                     GFENDAMAKAAKTWHGDWWKVFGGGTVWNGLTYDPELDLLYVGVGNGDPWDHDLRSQG
                     KGDNLYLCSIVALKADTGEYVWHYQVNPGEQWDYKATMDMILADLPMGGTTRKVLMQA
                     PSNGFFYVLDRATGELLGAEKYERATWAERIDLKTGRPVETPGIRMKPGEETLIYPGP
                     YGAHNWQAMSFNPGTGLAYIPTIRYPAIYSKNTAYEQREGFFVIGMNTVYATPEPGDG
                     KGGLVAWDPVKGEARWRIQYDSIWNGGTLTTSGGIVFQGTADGNFLAVDASSGEELWR
                     FDVQRGVTAAPISYTLDGRQHIVLPVGWGGQSAFGPPAFRAHRIPYKGEDVRLLSFSL
                     DGKASLPPPPARPTEIQTANTGDEPIDEALAVKGFNIYHQASCAVCHGGEAISVGAAA
                     PDLRESPYMANYPAFRAVVVEGALLPRSMPMFDDLTETEVRAVWEYVRQQTRLVD"
     gene            complement(10238..10663)
                     /locus_tag="AC16QF_08495"
     CDS             complement(10238..10663)
                     /locus_tag="AC16QF_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HIR6448638.1"
                     /translation="MPPSTPYQILGQEGINQLVDAFYDAMDELTEAAHVRAMHKENLE
                     EVRQKLKDYLIGWMGGPPIYQMKTGTVCLTDPHAPYAIGPKERDQWLLCMDTALERIG
                     ASDELKEMLKIPMFRVADTVRNRDSSEPKPRDPNIIAVG"
     gene            complement(10719..11618)
                     /locus_tag="AC16QF_08500"
     CDS             complement(10719..11618)
                     /locus_tag="AC16QF_08500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HIR6448639.1"
                     /translation="MKIVIAGGTGLIGRALSTRLAGAGHEVVVLSRQRQAPTGSTVAS
                     WDGRSPGAWQDVLRGAGALINLCGASIGEARWTPTRKRELADSRTLPSVALAQACAAV
                     EDPPKVLLQASGVGFYGTSETTTFDESSPPGEDFLARLAQEWENAGADLLPPETRRVV
                     MRMGVVLSREGGVLEQLLLPFRLFAGGPIGSGRQFLSWIHIDDLTAAMEFLIGTSQLS
                     GPVNLVAPEVPRNTQVARAIAEALHRPSWFPIPAWAMRLALGEMSSLVLEGQRVIPRE
                     LQAAGFRFSYPNLRSALAQILAN"
BASE COUNT         2010 a         3746 c         3947 g         2124 t
ORIGIN      
        1 gtgtggtagg cgtcgggacc caggcagcgg cgttggagtc tgccctgcga gccgccgagc
       61 ccgtatcgga atcagcgctg cagaatctgg ctgaagcgct ggcagaatcc tccacggcga
      121 tcgagcaact cgtggcattg cagtccgcgg cggaaggcgg tggcgaagag tctggcggga
      181 attcgctgga gctcctggac cgcctcgaaa aggaaattgg cgagaacgat ccggcagcct
      241 ctgacaccgc cgaggaactc ctcaagagcc tcgaggcgga ctctgccgcg gtcacggcgg
      301 ccaccgccgc tcgggatgcc ctggagctgt tcgactttgc cggtgccggc gagcagctgg
      361 accagattcg cgcgggtctt ggatcctgaa ttcttagcgg cctcaccggc tgtgctggca
      421 gcccggccac tcgccgccgg ttacggaacg cgaccggtta cgagaaactg gggcaatacg
      481 gccaggtagg ttttgtcgtc cagcgcgcgg tcgatgtcgg agatgaacgc atcgacgtcc
      541 gctctgtcga cctggccgga tgtgagcgcg taaccggcca tgttgtgcag aacgccgcgc
      601 agggcgccgg tcgtgtcggc ggcggcgagg acctgaacgc gcacatcgtc gaatccggcg
      661 cggcggaaca tgccgtacag gctgcggccg atcaggggtg tccgaaaagc caccgcggct
      721 gcggacatga tccgggcgaa cggctcgcgg gccggttcga agaccactgc accccagtcg
      781 ctgtcgctga cgtgggcaat gccgccgggc cgcagaattc ggtggaactc gttgatggtg
      841 gcctgtggat ccggcacgta ttcgagcacg ttcttgcaga tcacccgatc gaccgcgtgc
      901 gtgtccatgg ggaacctgtc gcccgtgacg agccgagtct cgacgcgctc tgctaccccc
      961 tcgtctgccg cccgggcccg ggcgcgctcg aggaattcgg cattgatgtc gagcccgatg
     1021 accttgcccg tgcttcccac ccgacgcgcc agctctacgg ctagcccccc gggaccacat
     1081 ccgtagtcca cgacgagctg gcccgcgccg atagcggccg gagcgatcag aacttcctgg
     1141 ccctcgcgcc aggcgaacat ggcctcatag cgcgccatgc gctcgtcttc gacgtcgagc
     1201 cagtggtcgc ggtagtaggt tgtcagatct gtcatggagg aaatcccatc aggtgagcgg
     1261 caattgggtc cttatcatcc gagttgctcg taaaccatcc gcttgaattc ctgactgaag
     1321 gggtgccaga agccgggcat tcgctgggtc atgcagacgc cggctatgcc ggcccgcggc
     1381 gccatccagg agtgggttcc cgcgatgcct ccccagtgat actcgccgac cgcagtctcc
     1441 ggttcgccgt cggcgggcgc ctgcttgagt gcaaacccga gcccgaagac gttgtctttc
     1501 atttcccaca tggggaagtg cacgctcacc ccgtcagcca gctggttggt ccgcatcagc
     1561 tcgagggttt cggcctggag cagccgcacg ccgtgccact ccccgccgtt gatcaacgcc
     1621 cgcagaaacg tcacgtagtc cgcgacggtg gagaccaggc cgtggccgcc ggccagcatg
     1681 gcgtggggtc tggagtacat gccgctcagc ggatcgtcgg ctttgttcag ccctcctttc
     1741 atgggcgcga acaggtcgac gggcgcatac atggtcgtaa accgatgctg cttctcggcc
     1801 ggcacgtgga agtcggtatc cgccattccc aggggatcca gaatgcgctc tttgagaaaa
     1861 acatcgaaac gctggccgga gatcacttcg atgaccctgg cggtgacgtc ggtgctgacg
     1921 ctgtagcgcc agttggtccc gggttgaaag gcgagcggaa gccggctgac cacctcacag
     1981 aggttctcga gcgtcccgtc ggtgtcgcta aggaggtgca gacctgcctg ggcgtagagc
     2041 tgatcaacta ccgaagtcgg ctcgaagacg ccgtaggaaa gcccggccga atggctcagg
     2101 atctgccgca tggtgatggg ggatcgggcg ggctcgacgt ctttcgcgga tttcgccccc
     2161 ggccgcagca cctgcgagtc ggagaacgac ggtatgaagc gggcaaccgg gtcgtccagc
     2221 tcgaagcgtt tctcctcgaa gagcatcatg gccgccacgg aggtgacgat cttggtgttg
     2281 gagtacatgc ggaaaatcgc gtcctcgcgc aggggctcgc gggattccag atccatgtag
     2341 ccgagcgtgc ggtagtcgag cacgtcggtg ccccgcatca cgaggcagac gcagtgaggg
     2401 atgatccgct catcgacgta ccactgcatc cgggcgtgca gggcagagaa gtcgatctgc
     2461 gtcggcagga tgtcgagcgt tgccatgatg tcgctcccgg gcgcggccag cggacgattc
     2521 taccgccagc cgtcccagaa tggcgtgtgt tgggtggccg ggcagaatgg tacgtgcaag
     2581 taccgtcccg gacgttccgg gaactcggcc attactctcg atgcggcgtc cgcggcagcg
     2641 gtattcgtca ccgagcttga agccgcccac cgggctggaa gcgaccccgc atgatagtgg
     2701 atgatggcgg gccagcttgc cgcggatttc gtacgcatga ccgattcgag gggcaatttc
     2761 attcccgggc tgctcgttgc catcggcctc gccgtcgccg gctacctgat aggtcaggga
     2821 ttcgtccacg cgcgggtttt agaccgcagc gtggaggtca agggtctggc cgagcgcgag
     2881 gtgccggcag acaccgccat ctggccggtg cagatcgtgg ccgctgggga cgatctgcaa
     2941 gcgcttctgg tgcagatcga ggggcagagc gggcgggtcg tcgggtttct cgccgatctc
     3001 ggctttacgc ccggggaagt cagcgtcggc gcgcccaacg tgctcgatcg tcaggcccag
     3061 aatttcggag atcccaacac ccgcttccgc tactcggtgg cccagacgat caccgtgttc
     3121 accgaagcgg tcgcgaccgt gcgggaggct ggcgggcggc tgctagagct cggcaaagag
     3181 ggcatcgtgg tgacccagaa caactacgac ctgcgcacgc agtacctctt caatggcctc
     3241 aacgacatca agccggaaat gatcgaagag gcaacccgga atgcccggcg cgttgcggaa
     3301 aagtttgctg gggactcgga cagcgtactc ggcaagatcc gatcggcccg ccagggtcag
     3361 ttttcgattc aggaccggga cagcaacaac ccgcacatca agcgcgtccg cgtggtatct
     3421 acgctcgagt actatctggt ggactgagtg gccgggcatt taccgcgccc gtctgctgtc
     3481 ggcggctaaa ggaggacaca ttgaacggca ttcacgacct cggcgggatg acgtgcttcg
     3541 ggcccatcgt tcgggaagac gacgagcctg tctttcactc ggactgggag cgccgcgtct
     3601 tcgcgatgaa catgctcagc atggggcagt tcggctccct cgatcaggcg cggcacgcca
     3661 tcgagcggat ggatccggca gcctatctga gcacgtctta ctacgagcac tggcttgccg
     3721 ggatcgaaat cctgggaagg gactcgggac tgctgatgga tgctgagatc gccagcggga
     3781 ggagcgagca cacctccacg ccgtcccgcc cgccgctgga tcccgaattc gtggacgcga
     3841 tggttcgctc cggatcgcca tcgacacgcc cggtgagcag acggccgacc ttcgacgtgg
     3901 gcgactcggt ccgcgcgcgg aatctggacg ttcggggaca cacccgcctt ccccggtacg
     3961 ttcggggaaa gcggggcgtc gtcagcgccg ttcacggcac gcatgtcttc cccgacacgt
     4021 cggcccatgg tctgggcgag aatcctcagc cgctgtacaa cgtgcgcttc gaggcggacg
     4081 agttgtgggg cgacaacctt gcgcgaaggg actgcgtcca catcgatctc tgggaagatt
     4141 atctggaggc gtcgcccgtg gaggggagat ctcatgagca atgaccacga caacgaccat
     4201 gaccatgacc acggtggtcc cccgaatccc tatcatgcgg cctcccgctc agacgatgaa
     4261 aaccgggcgc tcgaagagcg cgtcggtcgt atcgaatccc tgttgctgga gaaagggctc
     4321 gtcaggcagg acgcgctgga ccgcattgcc gacatatacg agaacgacct cggacccatg
     4381 aacggtgcca aggtggtggc tcgagcctgg gtggacgccg ccttcaagca gcgcctgctg
     4441 gaggacgcgt cggccgccat cggcgaactc ggctacggtg ggctccaggg ggagcatatg
     4501 gtggtggttg agaacacccc ggaggttcac aacgtcgtgg tctgcacgct ctgctcctgc
     4561 tatccgtggc ccgtcctggg cctgccgccc gcctggtaca agagcccggc ttaccggtcg
     4621 cgggtggtca tcgatccccg cagcgtgctc gaggagttcg gtaccgccgt ggacgacgac
     4681 gtggaggtgc gcgtgtggga cagcagcgcg gagatccgct atctggtgct cccggagcgg
     4741 ccgacgggga ccgaaacgct caccgaggat cagctggccg aactcgtcga gcgcgacgcg
     4801 atgatcggcg tgacccgggt tgccgcgccg tgaccggtga gcgggaagcc gcgcccctca
     4861 tcgcggagat gccggcaccg gcggcgctgc cgcgcaagag cggggaaatg gtctttcacg
     4921 atgagtggga acgccgtgcc ttcgccatgg cggtgagcct ggccgagcag ggcgctttcg
     4981 agtggtcgga gtttcagcag aagctcatcg cggcgatatc ggatgcggag cgggatgacc
     5041 ctcagcagcc cagccggggc tactacgaaa gctggctagt ggccctggag gctcttctgc
     5101 aggagaaaga gctgcttcca ggctagctgc tgcgcgccgt ggcgcgccct cggtcatcgg
     5161 tgagctcgcc gccggatgcg gtgagtcccc tgcggctgat accatcggcg ccgtcctttc
     5221 atgtggctgt ggttttgcgc ctgtatctga tcgtcctttt gctctgggcc gcgtccgctt
     5281 cagccgcgca ggccgattgg gagcctgtac ggaaggtgca cggtatccag gtcgagggtc
     5341 tgatgatgcc gtccggtttc cggtcccatc gtgctcaggt tgccgtttgc accgatcttg
     5401 cggatctgcg tgcgttcatt tccgacacct cgcgatttca cgagtggatt cccgatcttg
     5461 ccgaggtgcg cctgctcgag cagtccgacg acggggtcgt ctactacatg aggagcaagg
     5521 cgccctggcc ggtgaaagat cgagacatgg tgtaccggct cttcggcgtg cccgagtccg
     5581 gggacgctgc cagcgtggcg ctcgccatcg agggcctgcc cgggcggctg cccgaggtgg
     5641 atggcgccta tcgaatgcac gacgtttccg gtcaatggcg catatgggaa cggggtgacc
     5701 ggctcgacgt gcagcagcag atctacctca accccgggga cgtcccgcgg atgttcgcca
     5761 accgcagcat gtcggccagc ctggccagga cgctcggcaa tctggcctcg cgatacccct
     5821 gcccgaagag ctgaaggata gcagcgacgt cgcgcggcta ttgatacacg tcgatcatct
     5881 tggcatcggc gtggaagggc agccgctcgc agaaaccatc cttcagcggc agcgaggcgt
     5941 gccgccacat cggctcccag cagccgtgcc gctcgagcag ggttctggct tcggcctgct
     6001 catcctcggt gaggcccgcg aacgtctcgc gcagcttctt caggcaccag acccgatagg
     6061 gagaccagcg cgcacggtgg tagcgcacgc cgccgacctc cgcgtcgaag cgcctcaggc
     6121 ccgctgcaac cgcgtccacg ttggcgcaga ggtagggcag gtagtgcgtg ccgatctcgt
     6181 ccaggatggg cgcccagtcg tcgggaatac cggccggaat tgccatgctg gagtccacgg
     6241 ggccggcgtt ccacagccgg gcgatccact cgtagaccgc gggcgcgtct ctgcgcatga
     6301 tctcggcagg gaccggatcg agcccgaagt gacggaagaa ggggccggag aagccgacgt
     6361 ccgccaggct ggggctggca cccagcagga agggccggga tcggaagatc gtttcgagct
     6421 gtcccagggt ccgcgagtag atggcctcca cacccgggac ctggtcggcg gtgatgccgt
     6481 ctcccctggt gtagccgttg cgttgacgcc gggtgatggc gcgacgcttg acgaacatgg
     6541 gcaggtatac accccgcgtg agctcctggg ccagatgggc gctggcgaag cgagcgccaa
     6601 cgtcgtagaa ccagcggtag tgcatggccg gtcgccaaag ccactcgtcg gcgtaatctt
     6661 ccagcagcag gctgaagaac cgctgcaccg gatcgtgcgg aatcaccgcc ggctcgggat
     6721 actcccgctc gaaccacccg atgacgggcg tggtatcggt cagccagcgc ccgtcctcaa
     6781 gccgtaccgc gggcatctgc gaggatccgg ttctgctttt gatgagcttg cgaacagagc
     6841 cggggcccag gggtttgagc tcgtagggta tgcccttgat gcgaaggtag ttttccagct
     6901 tccccgtgaa gtacgagatg ctcgacccgt aaacggtgac cggcgcgatg ggctcatccg
     6961 tcatcgcctt gcccccggta taccgagatc tctacggcat ccggcaacga cgtgtactcg
     7021 atggtggccg ggcggttggc cgaatcgtag cgcccgcagt gacggacgta gtccaggtaa
     7081 gcgtccgcgc cgaacagctc gcccacgttg tctgctacca cgacggagcc cggcgcgatc
     7141 aacccttggg tttcgatcag ctgcagatcc tggaggtaga ggtccttcca atgatccaga
     7201 aacacgagat cgaagctccc ctcgagtgtc gggatcacgt cgctcgagct gccgtgcacc
     7261 acctctacgc gcccatcgag acccgcgtgc gctatgttgc cccgtgcggc ccgcacgttg
     7321 tcggcattca cctctacgga ggtgatgttc gtgcccggcc cgagaacgtc tgccatgtag
     7381 atcgacgagt aaccggcgta ggcgcccagc tccaggacgc gcggatcgcg gtccagcgcc
     7441 gctctggcct cggcaatgac cttgtccttc tccgggccga tgctcatcat ccagcgcacc
     7501 gacgttgcga actcgtcgag cttgcggagc acgtcctggg ggttgtgccg ttcggcatgc
     7561 gctgcgacgt gctccatggc tcgaagcgcc ctgggcggcg cgccggtggc gcggtcgatc
     7621 agggcgtgcc acatgttccg gctggtgaat cccagcgccg agggtgcgag aaagcgccac
     7681 atctgcatga tgggacctcc ttgggttgat gccgatttcg ctggcacgag atcgtaccgc
     7741 aaatcgcccg actgcttttg ccatcagccg ttagcggcta aggttgcggg ttaattgcca
     7801 accgacccgg tgacccgcca tggacgagat ccgcagctat cactacgacc ctgagaagtt
     7861 cgagccctac aggaagtggg cgctcgagga agccataccg tttctgaaag acaatctgaa
     7921 catcgtcggc ttctggctcg acaacggcaa agcgccggag gtcggcggca cgtcgcccat
     7981 ggacatgccg ctgggcagcg cgaacgtcac ctgggtcatc cgctgggaca gcgaagagga
     8041 ccgcgaagcg ggtcatgcca ggatattcgg cggccaggaa tggcaggaca tctggtcccg
     8101 tcaccccgac gccaacggct acctgcagat ggaggcgcgt ttcgccgatc ccgtctgagg
     8161 gggtcagtcc accaggcggg tctgctgccg gacgtactcc cacaccgctc gaacctcggt
     8221 ttcggtgagg tcgtcgaaca tgggcatgct gcgcggcagc agcgcgcctt cgaccaccac
     8281 ggcgcgaaag gcggggtaat tcgccatata cggcgactcc cgcagatcgg gggcggcggc
     8341 accgacggat atggcctcgc cgccatgaca aaccgcgcag ctggcctggt gatagatgtt
     8401 gaatcccttt actgccagcg cctcgtcaat gggctcgtcg cccgtgttcg ccgtttgaat
     8461 ctcggtgggc cgggctggag gaggcggcag gctggctttg ccgtcgaggc tgaagctcag
     8521 caggcgcacg tcctcgccct tgtaggggat acggtgtgcc cggaacgccg gcggcccgaa
     8581 cgccgactgc ccgccccagc ccaccggcag cacgatgtgc tgcctgccgt ccagcgtgta
     8641 gctgatgggc gccgcggtga cgccgcgctg cacgtcgaat cgccacagct cctcaccgct
     8701 ggaagcgtcc accgcgagga agtttccgtc tgcggtcccc tggaacacga tgccgccgga
     8761 cgtggtcagc gtgccgccgt tccagatcga atcgtactga atgcgccagc gtgcctcgcc
     8821 cttgacggga tcccaggcta ccagcccgcc tttgccgtcg ccgggctcgg gcgtcgcgta
     8881 gactgtgttc atcccgatca cgaaaaaacc ctcgcgctgc tcgtaggccg tgtttttcga
     8941 gtagatggcc ggatagcgga tggtgggaat gtaggccagc cccgtgccgg gattgaaaga
     9001 catggcctgc cagttgtgcg cgccgtaggg gcccgggtag atcaaggtct cctcgccggg
     9061 tttcatccgg atgccgggcg tctccacggg gcgcccggtt ttgaggtcga tgcgctccgc
     9121 ccaggtggca cgttcgtatt tctccgcgcc cagcagctct ccggtggccc ggtcgagcac
     9181 gtagaaaaac ccgtttgacg gcgcctgcat cagcaccttc cgggtggtgc cgcccatggg
     9241 taggtccgcc aggatcatgt ccatggtggc tttgtagtcc cactgctcgc cgggattgac
     9301 ctggtagtgc cagacgtact ctccggtgtc tgccttcagc gcgacgatgg agcagaggta
     9361 cagattgtcg cctttgccct ggctcctgag atcgtgatcc cacggatcgc cgttgcccac
     9421 gcccacgtac aacagatcca gctcgggatc gtaggtcagc ccgttccata cggtcccgcc
     9481 gccgaacacc ttccaccagt cgccgtgcca cgtcttggcg gctttcgcca tggcgtcgtt
     9541 ttcaaagccc agggacgggt ctcccggcac cgtgtaaaaa cgccagagca gctcgccatc
     9601 tgccgcgtcg taagcggaga cgaatcctcg ggttccgaat tctgctccgc cgttgccgat
     9661 gaagactttc ccgtcgaagg cacgcggcgc gccggttatc gctttgcgct cctcgcgccc
     9721 gaacgttcgc gtggcccatt tcaccctgcc ggtcctggca tccagcgaga cgagcctgcc
     9781 atcggcggtg ccgacgaaga tgctgccctc ggagtgggcg atgccgcggt tggtgttgaa
     9841 gctcaccccg attccccggg gcgcgtgctt ccagctctgg gggtcgtagg accagatgag
     9901 cttgccgctc cttgcgtcca cggcgtgcac gatggctcgg tcggcagaaa agtagagcac
     9961 accgtcgatc attaggggcg tgctgttgag cgacacggcg tccggcacgc tcagcgccca
    10021 gtcgagtccg agttccccca cgttgctccg gttgacctgg tcgatggggc tgaagcgttg
    10081 ttcggtgtgg tcgttgccgt agctcctcca ctcgacttcc gccacagccg aagcggctgc
    10141 cagcacagtg acgaacagag cgggcccggc aatgggcgag cgcggtctcg atcggtcgtt
    10201 catggacctg cgatggggtt tcgtttccag gatccgttca gcccacggcg atgatgttcg
    10261 gatcgcgcgg tttcggctcc gacgaatccc ggttgcgcac ggtgtctgcg acccggaaca
    10321 tcgggatctt gagcatttcc ttcagctcgt ccgatgcgcc gatacgctcg agggccgtat
    10381 ccatgcacag cagccactga tcccgctcct tgggcccgat ggcgtagggc gcgtgagggt
    10441 ctgtcaggca gacggtgccg gtcttcatct ggtagatcgg cggcccgccc atccagccga
    10501 tgaggtaatc cttcagcttc tggcggacct cttcgaggtt ttccttgtgc atggcgcgta
    10561 cgtgagccgc ctcggtgagc tcgtccatgg cgtcgtagaa agcgtccacg agctggttga
    10621 taccttcctg gccgagaatc tgataaggcg tcgatggcgg catggcggtc tcgcgtcggt
    10681 tgctgacgaa tcagcatacc caaggggatt acgggtaatc aattggccag aatctgtgcc
    10741 agcgccgatc gcaggttggg gtaggaaaag cgaaacccgg ctgcctgcag ctcgcggggg
    10801 attacccgct gaccctccag gacgagactg gacatctcgc ccagggccag gcgcatcgcc
    10861 cacgccggta tgggaaacca gctcggccgg tgcagagctt ccgcgatggc acgggcgacc
    10921 tgagtgttcc gcggtacttc gggcgcgacc aggttgacgg ggccggatag ttggctggtg
    10981 ccgatgagaa actccatggc cgcggtcaga tcgtcgatgt ggatccagga caagaattgt
    11041 cgaccggagc ctatgggtcc gcccgcgaag agccgaaacg gcaggagcag ttgctccagg
    11101 acgcctcctt cacgggacaa cacgacgccc atgcgcatga cgacgcgccg cgtctcaggg
    11161 ggcagcaaat cggcgcccgc gttctcccat tcctgtgcca gccgagcgag aaagtcctcg
    11221 ccgggcgggc tcgattcgtc gaaggtcgtg gtttcggagg tcccatagaa accgacgccc
    11281 gatgcctgca gcagcacctt gggaggatcc tcgacggcgg cgcatgcctg ggcgagagca
    11341 actgacggca aggtgcgcga gtcagccagc tcccgcttgc gagtcggcgt ccagcgggcc
    11401 tccccgatgc tcgcgccgca caggttgatc agggcgccgg cgccgcgcag cacgtcttgc
    11461 cacgccccgg gggaacggcc gtcccatgac gccacggtgc tgcccgtcgg cgcctggcgc
    11521 tgtcgcgaca gcaccactac ctcgtgaccg gcgccggcca gtcgggtaga cagtgcccgg
    11581 ccgatgaggc cggtgccgcc ggcgattacg atcttcacca gccggttacc aacgcgttcc
    11641 gttcaatgag ttgccgacct tagcggttgt gcccgccgaa gtgaatcggc gcggagctat
    11701 cagtggccgt gacctcgacg tcgctcgccg tggcggcctg gttcgcggtc gtagcggtga
    11761 ccgtgccggt cgtgagcgcg ccaatggtgc cagcggtggc ggtcgtagcg atgccatcgc
    11821 gcatgtc
//
LOCUS       DBNHLM010000185         7938 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1403633, whole genome shotgun sequence.
ACCESSION   DBNHLM010000185 DBNHLM010000000
VERSION     DBNHLM010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1403633"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="AC16QF_08505"
     CDS             complement(<1..603)
                     /locus_tag="AC16QF_08505"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017129865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6448640.1"
                     /translation="MHAMTQDTDQSRRAARLLEVLDLECIEQNLFLGANEVRGEFRLF
                     GGQVLSQSLRAACLTVEGRSAHSLHGYFMRAGNAGRPVLYEVDRIRDGRSFATRRVVA
                     IQSGEAIFSLDVSFQVDEAGFEHAHPMPNVPPPEELEGDIAVVSRLTGDDPHLSPMAG
                     RPRPFELRSVFKPGSEEWARNRHWNPVWIRFAGEVDPHDDP"
     gene            1015..2844
                     /locus_tag="AC16QF_08510"
     CDS             1015..2844
                     /locus_tag="AC16QF_08510"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6448641.1"
                     /translation="MAARQSINLWQLTRGQRLRYAGAIFAMGMTNLFIFGPVLVSGSA
                     IDVIADQDLSHAVPPLLWAASRLSEGAPFAAYLWVAAVVAVTMTAIGGAFLFARGRFA
                     AVASEAIVRNLRERLYDRLHDLEAGFFDTADTGDLVQRASSDVETLRVFLASDVVEIG
                     RAIMLIMCVAPILAWLDVRLAALSLCLMPLLAIGAYLFFARVKDVVQITDESEAAMTA
                     TLQENLTGIRVVRAFARQAFEIEKFGARNAEFRNNNRRLIQLMGIYWSTSDLFAMTQI
                     GIVLIAGGLFVLDGSITVGTLFEFMTMVSMVIWPVRQLGRVLTDTGKAVVSLGRINEV
                     LTEAEERAGTIPGEGRARGEIRIENLTFGYDPQRPVLEDVNLHVEVGETLAIVGPPGS
                     GKSSLIRVLLRMYPYQKGSVRIDGWEVAELDRLWLRHQIGVVLQDPFLYSRSIRANLA
                     LGRPGAPEKELIEACHDAAVHDAIIGFPEGFEAMVGERGVTLSGGQRQRMALARALLK
                     DPPILVLDDSLSAVDTGTERRILDAMRRRKGRQTTLIIAHRLSSVMHADRILVLQEGR
                     VVQLGNHATLAGEPGPYRTLCEIQGALDASIEADTNATGRGNG"
     gene            2837..4732
                     /locus_tag="AC16QF_08515"
     CDS             2837..4732
                     /locus_tag="AC16QF_08515"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6448642.1"
                     /translation="MDNYDDENFDDGFDDEALAARVDLKLWRKLFAYARRYPKDLVWL
                     AVCAFTTASMEVTYPLITKWVVDDVDAAYAAGTVISLWPHALAYLACTVTICIAIGGF
                     IWMGGKIRTHVSHDIRRDGFENLQRLSFAFYDYRPVGWLMARMTSDCERLSNILAWGF
                     LDLVWGSTMMLGIAVAMLVMNPKLALVVLAILPLLGWVSARFQRRILSSARRVRATNS
                     RITASFNEAIMGVLTSKAFVREQANLDEFRELTNTMHGASVLNLSQAAVYVPIVITLA
                     SLATGLALAIGGLDLLGGAIAIGTLVAFMAYTRHFFDPIEQLGHWFAEMQMAQASAER
                     ILSLIETDPAVKDSPDVIEALQRQADAPPRRNYAPDGGAANIRQIELRNVAFAYDPAK
                     PVLKDINLTINRGETVAVVGPTGGGKSTLVNVICRFYEPTAGQVLIDGVDYRNRSLHW
                     LQANLGMVLQNAHVFSGSIMENIRYGRLDASDEEVREAARLAGADEFVRALEKGYDTE
                     AGEGGNRLSAGQKQLVSFARAILADPQILVMDEATSSVDTHTEQRIQRGMANVLAGRI
                     AIVIAHRLSTIRNADRIIVVEEGRITESGTHAELMGRRGHYFELYKRQGLQESSATLS
                     DLEPRPA"
     gene            complement(4704..6104)
                     /locus_tag="AC16QF_08520"
     CDS             complement(4704..6104)
                     /locus_tag="AC16QF_08520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6448643.1"
                     /translation="MSKDYDVIVVGSGAAGLAAAVAAADAGASVLLVEADKRVGGSSR
                     LSGGHFYAAGTSTQREAGVDDDTADAMFEHYMTLNQWLVEPSVVRRYCDLSAPTFEWL
                     KGQGVSFSPQAVYRSGVGSVARGHPPEGSGEAVVNILDARRSSLGVDVVLDARVDGLL
                     LDDAGRVHGIRIGDDEATCGAVVMATGGFGANPEMLAEYYPAAAANGDWSWYIGTEMA
                     RGDGIRLGQDAGAAIDGHNRGLLLVTPGFSRDLEVLLPGWLVLINEQGRRFTDETAPY
                     TVLAGLIQHQTGKVYAVFDEAARRGAKRNPASQAYWVDEVLEQKADAGRILRADSLDE
                     LAAAAGIHADGFKGTVARYNADCEKGEDTAFFKSPETGMRAIEEGPFYAAEVRPAIIC
                     WTGTGLRIDAEARVLRTDEHPIPGLFAAGELVGSLHGDRYIGGGGSFGPCIVFGKLAG
                     ENAASYTLTQAWAQDR"
     gene            complement(6213..7202)
                     /locus_tag="AC16QF_08525"
     CDS             complement(6213..7202)
                     /locus_tag="AC16QF_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009045556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-thiouracil desulfurase family protein"
                     /protein_id="HIR6448644.1"
                     /translation="MADALPERPVPPLPIAISECLLGSKVRYDGSSSRSSFPHTLLDG
                     LFTYRGICPEVGIGMGTPREPIRLVGKPDDARVVGVKDPTMDVTERLIEFGRETAASL
                     TDVCGYVLMKNSPSCGLFRVKVYPVTDGEVTGAPDPRGRGAHAAAITAALPDLPAEEN
                     GRLHDPVLRENFVTRVFTYGHWQRCFGSADDLTAAKLVGFHSRYKYLLMAHSVPYYQA
                     AGRLLSDLRGDLPGKAAEYFSLLMRGLSRPATRKGHANVLSHLQGYFKRLLDGPSRQE
                     LEALIHSYRRGEQPLDAPLTLLKHHLRRHPQEYLEYQVYLDPHPGFAGLRRLL"
     gene            complement(7195..>7938)
                     /locus_tag="AC16QF_08530"
     CDS             complement(7195..>7938)
                     /locus_tag="AC16QF_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6448645.1"
                     /translation="HAAPLPLAEIMLGNRWLGSADRLVSVGLVDGDVVRDVPWGRRAD
                     AVIALDPSGGPPSVLEVTQVDSTGSNLAGEARDVLALTAALPLGSNEPGLELLALARV
                     AQMAGALERILELTLRYANEREQFGRPIARFQAIQHNLAVMAAEVAAAVRIADAAVDA
                     LGGERFEFEVAAAKSRVGEAAGVCAESAHQIHGAMGFTHEHQLHHFTRRVWAWREEFG
                     NEIYWRRRLGDHIAGLGADAVWDFLATRG"
BASE COUNT         1349 a         2718 c         2572 g         1299 t
ORIGIN      
        1 cgggtcgtcg tggggatcca cctctcccgc gaagcggatc cacacagggt tccagtgccg
       61 gtttctcgcc cactcttcgc tgcccggttt gaacaccgag cgaagctcga acggccgcgg
      121 ccggccggcc atggggctca agtgcggatc gtctcccgtc agccggctca ccacggcgat
      181 gtccccctcc agctcctcag ggggcggcac gttgggcatg gggtgcgcat gctcgaatcc
      241 cgcctcgtcg acctggaagg agacgtccag gctgaagatc gcctcccccg attggatggc
      301 caccacgcgt cgggtagcaa agctcctgcc gtcgcgaatg cggtccacct cgtagagcac
      361 gggtctcccc gcattgcccg cgcgcatgaa gtaaccgtga agagagtgag ccgagcggcc
      421 ctcgacggtc aggcaggcgg cccgcaggga ctgcgacaga acctgtccgc cgaagaggcg
      481 gaactcgccg cggacctcgt tggcacccag aaagagattc tgctcgatgc attccaggtc
      541 cagcacttcc agcaggcgcg ccgcgcgtcg cgattgatcc gtgtcctgag tcatggcgtg
      601 cattgtagat tcacgaggga atgacgaata cccacggccg gtaaccggat cctgcgggcg
      661 ccggccgcgc gcccggcggc ggctcccgac gcggtctcgt ggggcccagg cgttgaacct
      721 gcccacggat gccagtaagc ttgccgccct gtttgactca ccgtcccgtc aggaggccac
      781 catgagctgg atgaacagcc gaccgaccgt tgcccgatac ggagacaacg cgcgaaagcg
      841 gtgagcccgt ccttcgtctg aaatagaccc ccgccggcat ctgccggcct gtgatgagga
      901 cccgctcacc agcgagcgga tccccctgca gtcatcttcg accccgatcc gctccgcgcc
      961 tgcccaaccg ggcccaggac ccatgcaacc gagaatctgc gggagcgatt gacgatggca
     1021 gccagacaat cgatcaacct atggcagctg accagaggtc agcggctccg ctatgccggg
     1081 gccatcttcg ccatggggat gaccaacctg ttcatattcg gccccgtgct ggtgagcggc
     1141 tcggcgatcg acgtcatcgc cgatcaggac ctcagccacg ccgtcccgcc tctgctctgg
     1201 gcggccagca ggctttccga gggcgcgccc ttcgccgcct acctctgggt ggccgccgtg
     1261 gtcgcggtca ccatgacggc catcggcggc gccttcctgt tcgcgcgcgg ccggtttgca
     1321 gcggtggcgt cggaagccat cgtccgcaac cttcgcgagc ggctgtacga ccggctgcac
     1381 gatctggaag ccggcttttt cgacacggca gacacgggcg atctcgttca gcgcgcgagc
     1441 tcggacgtgg aaacgctgcg cgtcttcctg gcaagcgacg tggtcgagat cggtcgggcc
     1501 atcatgctca tcatgtgcgt cgcccccatc ctcgcgtggc tggacgtccg gcttgccgcg
     1561 ctgtctctgt gcctgatgcc cctgctggcc atcggagcct acctcttctt cgcccgggtg
     1621 aaagacgtgg tccagataac ggacgagtcc gaagccgcca tgaccgccac gctccaggag
     1681 aacctcaccg gcatccgcgt ggtacgggcg ttcgcgcggc aggcgttcga gatcgagaaa
     1741 ttcggcgcgc ggaacgcaga gttcaggaac aacaatcgcc ggctgatcca gctgatgggc
     1801 atctactgga gcacgagcga tctgttcgcc atgacccaga tcggcatcgt gctcattgcg
     1861 ggggggctgt tcgtgctcga cggttccatc accgtcggca cccttttcga gttcatgacc
     1921 atggtgtcca tggtgatctg gcccgtccgc cagctcggcc gggtgcttac ggatacgggc
     1981 aaggcggtgg tgtcgctggg caggatcaat gaggtgctca cggaagccga ggagcgggcc
     2041 ggcaccatcc cgggcgaggg gcgggcacgc ggggagattc gcatcgagaa cctgacgttc
     2101 ggctacgacc cgcagcgtcc ggtgctcgag gatgtgaacc tgcacgtgga ggtcggcgag
     2161 acgctggcca tcgtcggccc cccgggatcc ggcaagtcca gcctgatccg cgtgcttctg
     2221 cgcatgtatc cgtaccagaa gggatccgtg cggatcgatg ggtgggaggt cgccgagctc
     2281 gaccggctct ggctgcgcca ccagatcgga gtggtcctgc aggacccctt tctctactcg
     2341 cgcagcattc gcgccaacct tgcgctgggg cggcccggcg cgcccgagaa agagctcatc
     2401 gaggcatgcc acgacgcggc cgttcacgac gccatcatcg gcttcccaga aggtttcgaa
     2461 gccatggtcg gcgagcgcgg cgtcacgctt tccggcgggc agcggcagcg catggccctg
     2521 gcacgcgccc tgctgaagga cccgcccata ctggttctcg acgattccct ttccgcagtg
     2581 gatacgggaa ccgagcgcag gatcctggat gccatgcgcc gccgcaaggg ccgccagacg
     2641 accctgatca ttgcccaccg actgtcgtcg gtgatgcacg cggaccgcat tctcgttctg
     2701 caggaaggac gtgtggttca gctcggcaat cacgccacgc tggccgggga gcccggaccg
     2761 taccggacgc tctgcgagat ccagggtgcg ctggatgcat ccatcgaggc ggacaccaac
     2821 gccacaggac gcggcaatgg ataactacga cgacgagaac ttcgacgacg gcttcgacga
     2881 cgaagcgctg gcggccaggg tcgacctgaa gctgtggcgc aagctgttcg cctacgcccg
     2941 acgctacccg aaggacctcg tgtggctggc cgtctgcgct ttcaccaccg cctcgatgga
     3001 ggtcacgtat cccctgatta ccaagtgggt ggtagacgac gtcgacgccg cttacgcagc
     3061 cggcacggtg atctccctgt ggcctcatgc gctcgcctac ctcgcgtgca cggtgaccat
     3121 ctgcatcgcc atcggcgggt tcatctggat ggggggaaaa atccgcaccc acgtgagcca
     3181 cgacatacgt cgcgacggct tcgagaacct gcagcggctg tccttcgcct tctacgacta
     3241 ccgtccggtt ggctggctca tggcgcgcat gacgtcggac tgcgaacggc tctccaacat
     3301 cctggcctgg ggattcctcg acctggtctg gggatcaacc atgatgctgg gcatcgccgt
     3361 ggccatgctt gtcatgaacc cgaagctggc cctcgtggtg ctcgctatcc tgccgcttct
     3421 cggctgggtc tcggcccgtt ttcagcgccg gattctgtcc agcgcccgcc gcgtacgcgc
     3481 gacgaattca cgcatcacgg cttccttcaa cgaagccatc atgggcgtgc tgaccagcaa
     3541 ggcgttcgtg cgcgagcagg cgaatctgga cgagttccgg gagctcacca ataccatgca
     3601 cggcgcatcg gtgctgaatc tcagccaggc ggccgtctac gtgcccatcg tcatcaccct
     3661 ggcgagcctc gccacgggtt tggcgctcgc catcggcggg ctggatctgc tcggcggcgc
     3721 catcgccatc ggcaccctgg tcgccttcat ggcctacacg cggcacttct tcgaccccat
     3781 cgagcagctc ggccactggt tcgccgagat gcagatggcc caggcctcgg cggagcggat
     3841 actgagcctc atcgagacag acccggcggt gaaagactca cccgacgtga tcgaggctct
     3901 gcagcgccaa gccgatgcac ccccccgccg gaactacgcg cccgacggtg gggcggcgaa
     3961 catccgtcag atcgagctgc gcaacgtggc gtttgcctat gatccggcga aaccggtgtt
     4021 gaaggacatc aacctcacca tcaaccgcgg cgagaccgtg gccgtggtcg ggccgaccgg
     4081 cggcggcaag agcacgctgg tcaacgtgat ctgccgtttc tacgagccca ccgcaggcca
     4141 ggtgctcatc gacggcgtcg actaccggaa ccgcagcctc cattggctgc aggccaacct
     4201 cggaatggtt ctgcagaacg cccacgtttt cagcggctcg atcatggaga acatccgcta
     4261 cggacggctc gacgcgagcg acgaggaggt gcgggaagca gcccggctgg ccggcgcgga
     4321 cgaattcgtc cgcgccctgg agaaaggcta cgacacggaa gccggtgagg gcgggaatcg
     4381 tctttcggcg ggacagaagc agctcgtctc cttcgcccgc gccatactgg cggacccgca
     4441 gattctcgtc atggacgagg ccacctcatc ggtggatacg cacaccgagc agcggattca
     4501 gcggggcatg gccaacgtgc tggcgggacg catcgccatc gtcatcgccc accggctgtc
     4561 caccatccgc aacgctgacc gcatcatcgt ggtcgaggag ggccgcatca ccgagtcggg
     4621 cacccacgcc gagctcatgg gccgacgtgg ccactatttc gagctctata agcggcaggg
     4681 cctgcaggag tcgagcgcca ccctcagcga tcttgagccc aggcctgcgt gagcgtgtaa
     4741 gaggcggcgt tctcaccagc cagcttgccg aacacgatgc aggggccgaa agaaccgcca
     4801 ccgccgatgt agcggtctcc gtgcagcgag cccaccagct cgcctgcggc gaagagcccg
     4861 ggaatcgggt gctcgtcggt gcgcaacacc cgggcctccg cgtcgatgcg caggcccgtg
     4921 cccgtccagc agatgatggc cggacgaact tccgcagcgt agaacggtcc ctcctcgatg
     4981 gcgcgcatgc cggtttccgg gcttttgaag aacgccgtgt cctcgccctt ctcgcagtcg
     5041 gcgttgtagc gggccacggt gcccttaaag ccatccgcgt gaatgcccgc agcagcggcg
     5101 agctcgtcga gggagtccgc ccgaaggatg cgccccgcat cggccttctg ctctaaaacc
     5161 tcgtccaccc agtacgcctg ggaggccggg ttgcgtttgg ccccgcgcct ggcggcttcg
     5221 tcgaacaccg cgtacacctt tcctgtctga tgctggatga gccccgcaag caccgtgtag
     5281 ggcgccgtct cgtcggtgaa gcggcgtccc tgctcgttga tgaggaccag ccatcccggg
     5341 agcagaactt ccagatcgcg gctgaagccg ggcgtgacga gcagcagacc gcggttgtgg
     5401 ccgtcgatgg cggcaccggc gtcctgaccg agccggatgc cgtcgccccg ggccatctcg
     5461 gttccgatgt accacgacca gtcgccgttg gccgccgcag ccgggtagta ctccgcgagc
     5521 atctcagggt tggcaccgaa ccccccggtg gccatcacca cggccccgca ggtcgcctcg
     5581 tcgtcgccga tcctgatgcc gtggacccgc ccggcgtcat cgaggagaag cccgtccact
     5641 ctcgcgtcca gcaccacgtc cacgcccaga ctcgaacgac gggcgtcgag gatgttgacg
     5701 acggcctctc cggatccttc cggcgggtgg ccacgggcaa cgctgcccac cccggaccgg
     5761 tagacggcct gggggctgaa gctgacgccc tgacccttga gccactcgaa cgtgggcgcg
     5821 gacagatcgc agtagcggcg gaccaccgag ggctcgacga gccactggtt cagcgtcatg
     5881 tagtgctcga acatggcatc ggccgtgtcg tcgtcgaccc cggcctcccg ctgggtgctg
     5941 gtgcccgccg catagaaatg cccgccgctg agacgtgacg atccgcccac ccgtttgtcc
     6001 gcctcgacca gaagcacgct ggctccggcg tctgcagcag cgacggccgc ggcgagaccg
     6061 gctgcgccgc tccccacgac gatcacatcg tagtctttgc tcatggtgtc cctcccctgg
     6121 ctgcgttcac catcgcgtcg ggaagagttt accccgttaa gggcgacgcg ccaccggttg
     6181 gtcaacggca agggccccag ggcgcagaac gctcagagca atcgacgcag gccggcaaag
     6241 cccggatgag gatccagata gacctgatac tccagatact cctgagggtg acggcgcaga
     6301 tgatgcttca acagggtgag cggagcgtcc agcggctgct cgccacggcg gtagctgtga
     6361 atcagcgctt cgagctcctg gcggctgggc ccgtccagaa ggcgcttgaa atatccctgc
     6421 agatgggaga gcacgttggc gtgccctttg cgggtcgccg gtcgggacag gccccgcatg
     6481 agcagggaga aatactcggc cgccttgccg ggcagatcgc cgcgcagatc gctcaggagc
     6541 ctgcccgccg cctgatagta cgggacgctg tgcgccatga gcaggtactt gtaccggctg
     6601 tgaaagccga cgagctttgc ggcggtgaga tcgtcagccg agccgaaaca gcgttgccag
     6661 tgcccgtagg tgaaaacccg ggtcacgaag ttttcccgca gcactggatc gtgcagcctg
     6721 ccgttctcct ccgccggcag gtcgggcagc gcggcggtaa tcgccgcagc gtgagcgccg
     6781 cggccccggg gatcgggcgc gcccgtcacc tcgccgtcgg tgacagggta aaccttgacc
     6841 ctgaacaggc cgcagctggg cgagttcttc atgagaacgt aaccgcagac gtccgtcagc
     6901 gacgccgccg tctcccgacc gaattcaatg agccgctcgg tgacgtccat cgtcgggtct
     6961 ttcaccccca cgacccgcgc atcgtccggc ttgccaacga gcctgatagg ctcccgcggc
     7021 gtgcccatgc cgatgcccac ctccggacag attccccgat acgtgaacag accgtcgagc
     7081 agcgtgtgcg ggaacgacga tcgtgacgag ctgccgtcgt agcgtacctt gctgcccagg
     7141 aggcactcgc tgatggcaat gggcaggggc gggaccggcc gctctggcag ggcgtcagcc
     7201 acgcgtggcg aggaagtccc acacggcatc ggcacccagc ccggcgatgt ggtctcccag
     7261 gcgacggcgc cagtagatct cgttgccgaa ctcctcgcgc caggcccaga cgcggcgggt
     7321 gaaatggtgc agctgatgct cgtgggtgaa gcccatggcg ccgtgaatct gatgggcgct
     7381 ttccgcgcaa acgccggcag cctcgccgac cctggacttg gcggcggcaa cctcgaactc
     7441 gaaacgctca ccgcccaggg catccacggc ggcgtcggcg atgcgcaccg cggcggctac
     7501 ctcggccgcc atgacagcca gattgtgctg aatggcctga aagcgcgcga tggggcgacc
     7561 gaactgctcc cgttcgttgg cataccgcag ggtaagctcg agtatccgtt ccagcgcccc
     7621 cgccatctgg gccacgcggg caagggccag gagctcgagg ccgggttcgt tgctgccaag
     7681 cggcaaggct gcggtcaggg cgagcacgtc ccgagcttcg ccggccaggt tgctgcccgt
     7741 agagtcgacc tgcgtcacct ccagcacgga cgggggtccg cccgaagggt ccagcgcgat
     7801 cacggcgtcc gcccggcggc cccagggcac gtctctcacc acgtccccgt ccacgagccc
     7861 caccgacacc agacgatcgg cactgcccag ccaccgattg cccagcatga tctcggccag
     7921 cggcagcggc gcggcgtg
//
LOCUS       DBNHLM010000186         6102 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1523858, whole genome shotgun sequence.
ACCESSION   DBNHLM010000186 DBNHLM010000000
VERSION     DBNHLM010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6102)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1523858"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="AC16QF_08535"
     CDS             complement(<1..799)
                     /locus_tag="AC16QF_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012722828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR6448646.1"
                     /translation="MLINFFLTLRKYKVPVSIRELMDLLEALSLRLVYANIDDFYLLS
                     RTCMVKDEKHYDKFDRAFAAYFKGLEDLHGMLESLIPDEWLRREFEKSLTAEELEKIQ
                     SLGGLEKLIEAFQEARAEEERRQAEQARQGEEGEEGENPDRQGQKGPGGLGKGKKKKA
                     KKVWDQRQYKNLDDSVELGTRNIKMALRRLRKFARSGKEEELDIDTTIRSTAHNGGML
                     DIRMRPERKNTVKVLLFLDIGGSMDSHVKVCEELFSATRTEFKHMESF"
     gene            complement(841..1689)
                     /locus_tag="AC16QF_08540"
     CDS             complement(841..1689)
                     /locus_tag="AC16QF_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6448647.1"
                     /translation="MIFDSTDTYISTDELSMAVDAAVKLERPLLVKGEPGTGKTMLAE
                     EIAKSLGLRLIQWHIKSSTKAINGLYEYDAVSRLRDSQLGDERVQDIRNYIKKGKLWE
                     AFEADERVVLLIDEIDKADIEFPNDLLQELDRMEFFVYELGETIKAKQRPVVVITSNN
                     EKELPDAFLRRCFFHYINFPDRETMQQIVEVHHPSIKSELVKEAMEIFFDVRKVPGLK
                     KKPSTSELIDWLKLLMADDIPDEILTNRDTSKAIPPLYGALVKNEQDVHLLERLAFMN
                     RRDSRG"
     gene            complement(1940..2302)
                     /locus_tag="AC16QF_08545"
     CDS             complement(1940..2302)
                     /locus_tag="AC16QF_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448648.1"
                     /translation="MGNAWVWAAIVVGGLGAAAGLHMALSGLLAREIRLAVVFVLLCL
                     LITPAPVPEYAGSYAPALIILAFEGLFQRGGDPFTALKILLAVAALASGAAVLMVLGW
                     RRWASTSLPNAESADSSL"
     gene            complement(2302..2742)
                     /locus_tag="AC16QF_08550"
     CDS             complement(2302..2742)
                     /locus_tag="AC16QF_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HIR6448649.1"
                     /translation="MDRFWEHKTLGELSAEEWESLCDGCARCCLVKLEEEGSGCVHYT
                     GLVCDLLDLETCRCTRYPDRHRLVKDCIDFSAEVMASLSWLPRSCAYRRVAEGRGLAD
                     WHPLVSGSPDSVHRAGISVLGRVIPAGSVHEDEFEEHLIQWVET"
     gene            complement(2742..2993)
                     /locus_tag="AC16QF_08555"
     CDS             complement(2742..2993)
                     /locus_tag="AC16QF_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017526690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HIR6448650.1"
                     /translation="MSRLVQIYRSSRRNGMYLYVDAGEDLARVPEALMKSFGRPVAAM
                     SLALTRDRQLARADAAAVLKNIEECGFYLQMPPSVDELS"
     gene            complement(2990..4102)
                     /locus_tag="AC16QF_08560"
     CDS             complement(2990..4102)
                     /locus_tag="AC16QF_08560"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:NF013755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HIR6448651.1"
                     /translation="MPCRADAASVSWIDDTGALVDAVASWGDVIGLDTEFQRTDTFFP
                     VPGLYQVISGEDVYFLDPLSIDDFSAFADVLHDPAIVKVMHACSEDLELIRHHLGVEP
                     QGIFDTQLAYAFQNSRYSVSYANLVAALLGHDLGKHATRSNWLRRPLTEEQLHYAWED
                     VRYLPEVYEALTARLDELGRRSWFADAMAEKGRVQGAHPDEYYRGVKKAWRLDALELG
                     VLKRLCAWRERRAMAEDVPRNRVVWDEHLVEFARLGSLSETDVHERLPGPVARRYASE
                     LTAEHREGVSAGLAEPLLARPLSPSESKVSRTLRDLAWHKADTMEFARELLARKRDVE
                     ACIRHWLDTGELSPAYSGWRAGLIGEEFLAVLEHAP"
     gene            complement(4071..4670)
                     /gene="recR"
                     /locus_tag="AC16QF_08565"
     CDS             complement(4071..4670)
                     /gene="recR"
                     /locus_tag="AC16QF_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR6448652.1"
                     /translation="MGISPLIDRLIGAFQVLPGVGPKGAQRMVLHLLQRDREGARQLA
                     ETLLESLDRVSRCSRCRNLSEEAVCSICRDPRRDDDVLCVVESPADILAIEAAGGFRG
                     RYFVLHGRLSPIDGVGPEELGVDTLQRRVAELEPKEVILATNPTVEGEATAHYISKLL
                     KADVPNISRIAHGVPVGGELEYIDGGTIVHALQGRRSIS"
     gene            complement(4670..4987)
                     /locus_tag="AC16QF_08570"
     CDS             complement(4670..4987)
                     /locus_tag="AC16QF_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR6448653.1"
                     /translation="MKGLGDFMKQAQAMQERMQQVQEEIAKLEVVGESGAGLVKVTMN
                     GRHEVRRVHLDDSLLAEAKDVLEDLVAAACNDAVRKVEAAQRDKMAGMASGLGLPLDK
                     FPF"
     gene            complement(4984..>6102)
                     /locus_tag="AC16QF_08575"
     CDS             complement(4984..>6102)
                     /locus_tag="AC16QF_08575"
                     /inference="COORDINATES: protein motif:HMM:NF023592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HIR6448654.1"
                     /translation="LEEPPEHVKFLLATTDPKKVPVTVLSRCLQFQLKNISSERIAAY
                     LAEVLQAEEISFEPEALETIAAAALGSMRDALSITDQAIAYGGGSLGHADVVEMLGLV
                     GRDEVHSLLEALASGDAERVLAMTAELAERGADFEEVLSGVIAAFHRMAVEQATGSGA
                     NALFGPEVVQLYYQIALMGYRDMHIVPDPRSGFEMTLLRMIAFAPDEGLLTQSTAPST
                     TAERGAKERPVPESAGGRDGVESTLADGTPWHEVVESLEIGGVARMIAEHSVVGSHQG
                     DRLRLTLDTAHDTLLNSAQQAALQRALGGKLGSDLLLEIEAGSCAAETPAQRNARIAA
                     ERQHQAEQTLKRDGVVQSLLSEFDGTLGDVKPMEDSAG"
BASE COUNT         1109 a         2029 c         1797 g         1167 t
ORIGIN      
        1 agaagctttc catgtgcttg aactcggtcc gggtcgccga gaaaagctcc tcacagacct
       61 tcacatgaga gtccatggaa ccgccgatgt cgaggaaaag aagcaccttc accgtattct
      121 tgcgctcggg ccgcatgcgt atgtcgagca ttccgccgtt gtgcgccgtc gaccggatcg
      181 tggtgtcgat atccagctct tcttccttcc cggaacgggc aaacttcctc agccggcgca
      241 gcgccatctt gatgttgcgg gtgccgagct ccaccgagtc gtcgagattc ttgtactgcc
      301 gctgatccca gactttcttt gctttcttct ttttgccttt gccaaggccg cccgggccct
      361 tctggccctg ccggtcggga ttctcgccct cctcgccttc ctcgccctga cgcgcctgct
      421 ctgcctgacg ccgttcctcc tcggccctgg cttcctgaaa cgcctcgatg agcttctcca
      481 ggccgccgag ggattgaatc ttttcgagct cttcggctgt caacgatttt tcgaattcgc
      541 gacgcagcca ctcgtccggt atgagggact ccagcatccc gtgcaggtcc tccagccctt
      601 tgaagtaggc tgcaaaggcc cggtcgaact tgtcgtagtg cttctcgtcc ttgaccatgc
      661 aggtgcggct gagcagatag aaatcgtcga tgttggcgta aaccagccga aggctgagcg
      721 cctccagcag atccatcaac tcgcggatgg agaccggcac cttgtatttg cgcagggtga
      781 gaaagaagtt gatgagcatt gtccggtcgt gctccctcgg tggctatcga cccgccgctt
      841 ctagccgcgg ctgtcccgac gattcatgaa cgccagtctc tcgagcaggt gcacgtcctg
      901 ttcattcttc accagggcgc cgtaaagtgg cggtatggcc ttgctcgtat cccgattggt
      961 gagaatctcg tccggaatgt cgtccgccat cagcagcttc agccagtcga tgagttcgga
     1021 tgtggacggc tttttcttca gccccggcac cttgcgcaca tcaaagaaga tctccatcgc
     1081 ttccttgacc agctcggact tgatgctcgg atgatgcacc tcgacgatct gctgcatggt
     1141 ctcccgatcc gggaaattga tgtagtggaa gaagcagcga cgcaagaagg catcgggcag
     1201 ctccttttcg ttgttcgacg tgatgacgac aaccggccgc tgcttcgcct tgatggtttc
     1261 gccgagctcg tagacgaaaa actccatccg atcgagttcc tgaagcaggt cgttcggaaa
     1321 ctcgatgtcc gccttgtcga tctcgtcgat cagcagcacc acgcgctcgt cggcctcgaa
     1381 agcctcccag agcttgccct tcttgatgta gttgcgaatg tcctgaaccc gctcgtcgcc
     1441 cagctgggaa tcgcgcagcc gcgacaccgc gtcgtactcg tagagcccgt tgatcgcctt
     1501 ggtgctggac ttgatgtgcc actgaatcag tctgagccca agcgacttgg cgatttcctc
     1561 ggccagcatg gttttgccgg ttcccggctc gcctttgacg agcagcgggc gctcgagctt
     1621 gaccgcggcg tccacggcca tgctcagctc atcggtcgag atataggtgt cggtactgtc
     1681 gaagatcatt cggctctact cgtgaattga tgtttcgcgc gagtgaagga ccccccagaa
     1741 aactgcgtca gcaggcgtcg cgcgtacgaa gtccgctact ttaatcgacg ctccgaggcc
     1801 tagcaaggcg cggcccagcc tgagccccgt gcgaccggga gcatgatcct gtccgcaagc
     1861 tcattgccgg acgaccaacg caaccgggag ttagatggca atgataaata tatataaaac
     1921 aataatttgt agatcttttc tagagactgc tgtcagcgct ttccgcgttc gggagggacg
     1981 tgctcgccca gcgccgccag ccgagcacca tcaacacggc ggcgcccgag gccaacgctg
     2041 ccacagccag gaggatcttg agcgcggtga aaggatcgcc gccccgctga aagagccctt
     2101 cgaaggcgag gatgatcaga gccggcgcgt agcttccggc atactctgga accggcgccg
     2161 gcgtgatgag caggcacagc aacacgaaaa cgaccgccag cctgatctcg cgggcgagca
     2221 acccgctcag cgccatatgc agccccgctg cggcacccag gccgcccacc acgatagccg
     2281 cccataccca tgcgttcccc atcaggtctc aacccactgg atcagatgtt cttcgaactc
     2341 gtcctcatgc accgagcccg ccgggatcac tcgaccgagc accgagatgc ccgctcgatg
     2401 cacggaatcc ggggagccgg aaacgagagg gtgccagtcg gcgagccccc gaccttcggc
     2461 gacacggcgg taggcacagc tgcgcggcag ccaggagagc gacgccatca cctccgcgga
     2521 gaagtcgata cagtccttga cgaggcggtg tctgtccgga taacgggtgc agcgacaggt
     2581 ctcgaggtcc agcagatcgc aaaccagccc cgtgtagtgg acgcatcccg aaccctcctc
     2641 ttcgagcttg acgaggcaac atcgggcgca gccatcgcag agcgactccc actcctcggc
     2701 cgacagctca ccaagcgtct tgtgctccca gaaccggtcc atcaggaaag ctcgtcgacc
     2761 gatggcggca tctggaggta gaagccgcac tcttcgatat tcttgagcac ggccgctgca
     2821 tccgccctgg ccagctgccg gtccctggtc agcgcgaggg acatggccgc caccggtcgc
     2881 ccgaagcttt tcatgagcgc ttccggtaca cgggcgaggt cctcgccggc gtccacgtac
     2941 agatacatgc cattccggcg cgagctgcga tagatctgca ccaggcggct caaggcgcgt
     3001 gctccagaac cgccaggaac tcctcaccga tcaagccggc ccgccagccg gagtaggcgg
     3061 gcgacagctc gccggtgtcc agccagtggc gaatgcaggc ttccacgtca cgcttgcggg
     3121 cgagcagttc cctggcaaac tccatcgtat cggccttgtg ccaggcaagg tcgcgcaggg
     3181 tccggctgac cttgctctcc gacgggctca agggtcgagc gagcagcggc tcggcgagcc
     3241 cggccgagac gccttcccga tgctcggctg tgagctcgga ggcgtaccgg cgggcaacag
     3301 ggcccggcag acgctcgtgc acgtccgtct cagaaagcga tccgagacgc gcgaattcca
     3361 ccagatgctc gtcccagacc acccggttgc gaggcacgtc ttcggccatt gcccggcgct
     3421 cgcgccacgc gcaaagccgc ttgagcacac cgagctcaag agcgtccagg cgccaggcct
     3481 tcttgacgcc acggtagtat tcatccggat gggctccctg aacccgacct ttctcggcca
     3541 tggcgtcagc gaaccacgag cgtctgccga gttcgtcgag ccgcgcggtc agcgcttcgt
     3601 aaacttcggg aaggtagcgc acatcctccc aggcatagtg cagctgctcc tcggtcaggg
     3661 gccggcgcaa ccagttggac cgggtggcgt gcttgcccag atcgtgtccc agcaatgccg
     3721 ctaccaggtt ggcgtagctc acgctgtacc tcgagttctg aaacgcatag gccagctggg
     3781 tatcgaatat cccctggggt tccaccccga ggtgatgacg aatgagctcg aggtcctcgg
     3841 agcaggcgtg catcaccttg acgatggccg gatcgtgcag cacgtcggca aacgccgaga
     3901 aatcgtcgat ggacagcggg tcgaggaaat acacgtcctc gccgctgatg acctgataaa
     3961 ggccgggcac cggaaaaaag gtgtccgtgc gctgaaactc cgtgtccaga ccgatcacgt
     4021 cgccccagga cgccaccgcg tcgaccagag cgccagtgtc gtcgatccag ctaactgatg
     4081 ctgcgtcggc cctgcaaggc atgaacgatc gttcctccgt caatgtattc gagctccccg
     4141 ccaacgggca ccccgtgggc gattcgcgat atgttgggta cgtcggcctt cagcagcttg
     4201 ctgatgtaat gcgccgtggc ttcgccctcc accgtggggt tcgtggcgag tatgacttcc
     4261 ttcggttcca gctccgcgac cctgcgctgc aacgtgtcta cgccgagttc ctcgggcccc
     4321 accccatcga tgggggacag ccgtccgtgc aggacgaagt agcgtccgcg aaagccgccc
     4381 gccgcctcga tggcgagaat gtccgcgggc gactcgacca cgcagagaac gtcatcgtcg
     4441 cggcgcggat ctctacagat cgagcagacg gcctcttccg acaggttccg gcagcgtgaa
     4501 cagcgcgaca cgcggtccag ggactcaaga agcgtctccg caagttgccg ggcgccctca
     4561 cgatcgcgct gcaacaggtg caggaccatg cgttgcgctc ccttggggcc gactccggga
     4621 agcacctgaa aggccccgat cagccggtcg attagcggac tgattcccat cagaaaggga
     4681 acttgtcgag cggcaggccg agaccggatg ccattcccgc cattttgtcc cgttgggccg
     4741 cttccacctt ccgaaccgcg tcgttgcagg cggcagcgac caggtcctcg agcacgtctt
     4801 tcgcttccgc aagcaggctg tcgtcgagat ggacgcgccg aacttcgtgc cgcccgttca
     4861 tcgtgacctt gacgagaccg gccccgctct cgcccacgac ttcgagcttc gcgatttcct
     4921 cctgaacctg ctgcatgcgt tcctgcatgg cctgggcctg tttcatgaag tcaccgagcc
     4981 ctttcatcct gccgaatcct ccatgggttt gacgtctccc agggtgccgt cgaactcgct
     5041 gagcaacgac tgcacgaccc cgtctcgctt gagggtctgc tcggcctgat gctgacgctc
     5101 cgccgcaatg cgcgcgtttc gctgcgccgg tgtctccgcc gcgcagcttc ccgcttcgat
     5161 ctcgagcagc agatctgatc ccaacttgcc tccgagggcg cgctgaagcg ccgcctgctg
     5221 ggcggagttg agcaacgtgt catgggccgt atcgagcgta agccgcagcc ggtccccttg
     5281 gtgggaaccc accacgctgt gctctgcaat catccgtgcg acgccgccaa tttcaaggga
     5341 ctcgaccacc tcgtgccacg gcgtgccgtc ggccagggtc gactcgactc cgtcgcgccc
     5401 cccggcgctt tccggcaccg gccgctcctt tgctccccgt tcggccgtcg tcgagggcgc
     5461 agtgctttga gtcagaaggc cctcgtcggg agcaaacgca atcatacgca gcagggtcat
     5521 ctcgaaaccg ctacgcggat ccggaacgat gtgcatgtcc cgatagccca tgagcgcaat
     5581 ctggtagtag agctgaacca cctccggccc aaagagcgcg ttggctcctg aacccgtcgc
     5641 ctgctccacg gccatacggt gaaaggccgc gatcacgccg cttagaactt cctcaaaatc
     5701 cgcccctcgc tctgccagct ccgccgtcat ggcaagcacc cgctcggcgt cgccactggc
     5761 cagtgcttcc aacaggctgt gcacctcgtc gcgtcccacc aggccaagca tttctaccac
     5821 gtcggcgtgg ccgagagacc cgccgccgta agcgatggcc tgatcggtga tggaaagcgc
     5881 gtcccgcatg ctgcccagcg cggccgcggc gatggtctca agtgcctcgg gctcgaagga
     5941 gatctcctct gcctggagca cctcggcaag gtaggcggcg attcgctcgc tggagatgtt
     6001 tttcagctga aactgcaggc agcgggagag gacggtgacc ggtacctttt tcgggtccgt
     6061 cgtcgccagc agaaacttca cgtgctccgg cggctcctcg ag
//
LOCUS       DBNHLM010000187        13354 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1642987, whole genome shotgun sequence.
ACCESSION   DBNHLM010000187 DBNHLM010000000
VERSION     DBNHLM010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13354)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13354)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13354
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1642987"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..890)
                     /locus_tag="AC16QF_08580"
     CDS             complement(15..890)
                     /locus_tag="AC16QF_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR6448655.1"
                     /translation="MARTLRFIIIPKVEHPWFDEVNNGAQAQAEILSRELGVEIVVDY
                     MPPSICDVVEQNTILENAAKSRPSGIAIDPVDVVDHMTAINRVRDQSIPVVLFDSPSP
                     DGSITSVGNNFAQQGVIAAERLAKLIGYTGKVAVMQGYPTAPNHKERYEAQVAVLKRY
                     QDITAVDGGIDNDDIETARQQASSVLESHPDLSGYLCCDASGPIGIATAIKEAGKAGE
                     VKVVGMDGIKPILDAIKQGVIDSSSATIPKMQGSMSILMLWQASLGVQMPQAIDTGID
                     VITQENVDRYLADAV"
     gene            complement(961..1434)
                     /locus_tag="AC16QF_08585"
     CDS             complement(961..1434)
                     /locus_tag="AC16QF_08585"
                     /inference="COORDINATES: protein motif:HMM:NF025292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HIR6448656.1"
                     /translation="MVLSAAWMVMSVGAVSAQTTKDLVGTWMVVSAFTEQGGTKTETY
                     GPNPKGILIVTDNGRYAIAAMRVGLPKLASNDRETGTPEENKAIVAGSIAHFGTLSVN
                     AAEKAITLKVDAATFPNWDGSEQKWPFTLVGDELKFTVPAASGGGTAVTVWKRAK"
     gene            2211..2714
                     /locus_tag="AC16QF_08590"
     CDS             2211..2714
                     /locus_tag="AC16QF_08590"
                     /inference="COORDINATES: protein motif:HMM:NF016847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR6448657.1"
                     /translation="MLHTISTRLARLLERQGLLVRDAESDYLDLEPGEALDQLIGASI
                     RSRIAIGPNAGRKALTLRTVPAQPEPLASTLLAKQPGFSLHAATCCEAKQRDKLEKLC
                     RYITRPAIANERRSTNERGQVIYRFKQPFRGGTTHVVLDPFDFISRLAVLVPRPRHKH
                     PSNHGHP"
     gene            complement(3047..3220)
                     /locus_tag="AC16QF_08595"
     CDS             complement(3047..3220)
                     /locus_tag="AC16QF_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448658.1"
                     /translation="MSDELTAFRQEAREWIAGNFPGSLKGGGGGGLSQAHARIINEEL
                     TGIGSMTARSQAI"
     gene            complement(3250..5628)
                     /locus_tag="AC16QF_08600"
     CDS             complement(3250..5628)
                     /locus_tag="AC16QF_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6448659.1"
                     /translation="MSAMYKNTCGYRWLTLVILIWLGSAQAAVLEEIVVVAQKRDQSL
                     QDVGIAVSAFTGDQLDQLGWDTAEQVTAMSPGVTTIQPNGPSAFFTNIRGVAQNDFSG
                     DHQESPVAVYVDEVYISAASGAGFQLFDFERVEVLRGPQGTLFGRNATGGLVHYITRK
                     PSQELDGYIKGTYGDYDQLKFEGAVGGGITDKISGRLSFTTNNHDGIYKNRIGSNLND
                     GDDWAIRGQVLIDFNDRTSWLLNLRRGEQDVNVAPFSHRSARLNPITGLGEDFDGPDL
                     TAEGDSRITEGYQDPDQKLLKGSVNVEGFNQIETTGFTSNLEFGIGDTMSLVFVTDYS
                     ELDKDYIEDSDAGPEDFFAFTLRSDIEQFSQEVRLAGSTDTSNWVVGAYYLDIQGEFF
                     NGGAASNFFAAAFPGFGLNDPSLETLGLNNPFSTNTESVALFAQVEYDFTETLRGTVG
                     VRWSKEKKDMDFQQFFSLFEAPDSFEIDVEDGLGLGGAIWTFSPDAVTNEPGGPAFDF
                     DPVNGDPGLAKLNDDLITAKLALEWLPSDNLLSYISYNRGIKAGGFNAPIDATDFYAG
                     VREPEEMRFDEEVLNAYEIGFKWTFAGGLARLNGAAYYYDYQDYQAFALDSLTTVVFN
                     TDAETYGLELELQASPVEGLDLLLGLGLIDNTVEDGHTQPDGTPVDRTAVLTPEVNIS
                     GIVRYQWSALGGMLAVQGDVNYMSSHYFQLKNSPVGKESGYAITNARLSWMSEKGDWD
                     VSVFVNNLFDEDHRLMVFDLAGSPAEGGFGMYENYAGNPRWWGASVRYAFGF"
     gene            complement(5764..6237)
                     /locus_tag="AC16QF_08605"
     CDS             complement(5764..6237)
                     /locus_tag="AC16QF_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448660.1"
                     /translation="MDHQALAAMLGRYGEFFGAIAVVATLGYLALQVRQNTRSSYVTR
                     SAAVTNRLGEIQEAIVCNSDLSALMAKCRDPELTDLTPTEEERVQGIAELYVKTFSDV
                     QTAHSNSEMTDAYFATFRAEFRRIVETYPALKPRMRLTLKRFDMKLFPIYAPALD"
     gene            6338..6829
                     /locus_tag="AC16QF_08610"
     CDS             6338..6829
                     /locus_tag="AC16QF_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448661.1"
                     /translation="MDIQGIAALLGNFGEFFGAIAVVATLAYLAVQIRQNTNALRSAS
                     YEHWNQQAAEFAHFQDQHAKELAETEDLASFDELTGEQFRYVACLLTIAANQAQCAFL
                     QHRAGTVDDDVFEARIRAFLFFIEKIPHLKETWGLITRESEVPAFVEYIEERASWLRP
                     ATA"
     gene            6910..7425
                     /locus_tag="AC16QF_08615"
     CDS             6910..7425
                     /locus_tag="AC16QF_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448662.1"
                     /translation="MERLNQWLGLVANFGVVIGIVFLAYEMRLNTDSMKSSTAAQSMA
                     AWNEIVLAAATNPDLIAEQIAANREGIRLDAGGLQISYFAGSMLKSTEFNYLEHARGN
                     LEQEQWDAYVYAQRWFMTYNTFLLHAWRMQRYTFASSFREFMDDLIRDICSKQECPEG
                     GRPEDWTELST"
     gene            complement(7786..9066)
                     /locus_tag="AC16QF_08620"
     CDS             complement(7786..9066)
                     /locus_tag="AC16QF_08620"
                     /inference="COORDINATES: protein motif:HMM:NF046778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6448663.1"
                     /translation="MKLEEAKVAVIGGSLAGCSIAIVLRRAGCRVDVYERSPTDLQDR
                     GAGIVLPNQLQSELIDRGFLPPDYPGCPIRQRDWVVHDGSFEGRVVWRQPVSGVANHW
                     GRLWRALRAAIPDACYHNGLGLIDYANSAEGISFQTSDGERRRADLLVAADGYRSLVR
                     EQMTWGSESDYAGYVLWRGNFEDSRVADRAVIARLDERQSWLSACYDGGHSVLYMIPG
                     AAETPGQDERRINWGIYSRPPDGLVLHEPSSIPEGSVTEAMYAQFDALLGDSLPAQIA
                     ALIRLSPREQVSIQPIYDELAVHFSEARVMLVGDAAAVVRPHTASGATKALQDALVFE
                     RLSREHDDLDALLAAYGAERLENCNALVEVGRRIGRAQVEETPEWSAMSPADFARWNE
                     ATLAGQSLYLYEDVSNTGGEAESHAGEPDVPPGP"
     gene            complement(9118..9276)
                     /locus_tag="AC16QF_08625"
     CDS             complement(9118..9276)
                     /locus_tag="AC16QF_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448664.1"
                     /translation="MTKGNQDSVEKTVRDIRRATRRKHNPEEKIRIVLEGLRSEQSIE
                     PGGFTHAR"
     gene            9645..10598
                     /locus_tag="AC16QF_08630"
     CDS             9645..10598
                     /locus_tag="AC16QF_08630"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6448665.1"
                     /translation="MTADQEIVTARGTVGETLRVPTANPRNFFEAISDPGAMERTEIL
                     GKLFLPAGQGPWPVVVIVPGSLGIAPSHVAKADLLNEAGIAACLIDPFGTRGVTSTVA
                     NQAQFSFAASAWDVLASVAVLAAREAIDAARIGAQGHSRGGSAVLSAACMARLETVDV
                     RSGLRAIYAAYPWSGQQFLSPFVGDTLVRAVIGDQDEWCLAQQVQGHIQAIRLCGGDA
                     SCRIFAGAHHSFDRGTPLELIAEASVSPGAPTVYIREDGAFVHPADGRADPAFTDREL
                     MMYGIKAGYGRRGAHLGSEGDQADRFHEDMMAFWIKALGVG"
     gene            complement(10595..11035)
                     /locus_tag="AC16QF_08635"
     CDS             complement(10595..11035)
                     /locus_tag="AC16QF_08635"
                     /inference="COORDINATES: protein motif:HMM:NF016955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HIR6448666.1"
                     /translation="MSMKDKILGTGFARFKDRRFLEASMAASALVAMADAHVHLSEQL
                     AVDRVLENIERLNVFDPRKAIDLHRQFVQAMRSNSGEGQDWALEVIGRFRGNEEAADL
                     LIRIARYTAQADQHLSGRELMAIRRLCRELGVDPEPYMEDADPD"
     gene            11211..12053
                     /locus_tag="AC16QF_08640"
     CDS             11211..12053
                     /locus_tag="AC16QF_08640"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448667.1"
                     /translation="MRELDGKVAVVTGAASGIGRATATRFAGEGMKVVMADIEEAALE
                     RAVAELSGAGHEVLGVPTDVSDWEAVSLLADKTIDAFGSVNVVHNNAGVVVSGPIADL
                     TLADWEWVLGVDLWSVVYGVKAFLPLIREAGEGHIVNTASTAGLQASASIAPYNVAKF
                     GVVALTETLRAELDSEGSAISASVLCPGAINTQIVNSKRNRHSASAHDHQTSDAERAF
                     ESRAGRLLAEEGMDPAEVADMVVNAIRGNEFWIVTHPQWIDVLKSRVDAMARDRSLAR
                     GFGG"
     gene            complement(12110..12186)
                     /locus_tag="AC16QF_08645"
     tRNA            complement(12110..12186)
                     /locus_tag="AC16QF_08645"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12150..12152),aa:Val,seq:cac)
     gene            complement(12257..>13354)
                     /locus_tag="AC16QF_08650"
     CDS             complement(12257..>13354)
                     /locus_tag="AC16QF_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6448668.1"
                     /translation="VDPLRRYPSPMLKLKCSVRKAVLIGALAGASPAMAGAPEHHTES
                     GRFTNPYVEQAQGGVFAFLKRRLLGQDTWSSYDAHRDGAVPTAAPRLVAEGEQTNNAR
                     VTWVGHATVLIQHAGVNVLTDPILSRIASPVAFAGPGRKSAPGVDAQGLPPIDVVVIS
                     HDHYDHLDEPTIRFLGDRPRYFVPLGLKQWLVDAGIDQSRVTELDWWEKQTVEPGGSA
                     LTVTATPTQHVSGRTLTDRNKRLWAGWAMEWDDFTVWFGGDTGYNDVQFKAIGDRFDG
                     FDLGIVPIGSYAPRAYMHSYHADPEQAVRIHQDIRARRSMAVHWGTFHLTAEPLLEPP
                     ELLRAAVLEAGLPSEEVTAYAVGESRAYFPL"
BASE COUNT         2605 a         4150 c         3903 g         2696 t
ORIGIN      
        1 gagatatcgg gtctttacac tgcatcagcc agatatctat ccacgttctc ctgtgtaatc
       61 acgtcaatgc ccgtatcaat agcctgaggc atctgaacgc ccagcgatgc ttgccacaac
      121 atcaggatcg acattgagcc ctgcattttc ggaatggttg cggaggagga atcgatgacg
      181 ccttgtttta tggcgtcgag tatcggctta atcccatcca tgccgacgac ttttacttcg
      241 ccggccttcc cggcctcttt gatagcggtt gctatgccga tcggaccgga cgcatcacag
      301 cacaagtaac cacttagatc gggatgcgat tcgagaacag acgaggcttg ttgtcgagca
      361 gtctcgatgt cgtcattgtc aattccaccg tctacagcag ttatgtcctg atacctcttc
      421 aatactgcca cctgggcttc gtatcgttcc ttatggtttg gtgctgtggg atatccctgc
      481 atcacagcca ctttgccagt gtatccaatc agcttggcga gacgttcggc cgctatgact
      541 ccctgctgag caaagttgtt gccaacgctg gtgatgctgc catccggcga tggtgagtcg
      601 aagagaacca ccggaatact ttgatccctg acacggttga ttgccgtcat gtggtccaca
      661 acgtcaaccg gatctatggc gatgccactc gggcgactct tggcggcatt ctccaagatc
      721 gtgttctgct cgacgacgtc gcaaattgat ggcggcatat agtcgacgac gatctcgaca
      781 ccaagctccc ggcttaggat ttcggcttga gcctgggcac cattatttac ctcgtcgaac
      841 cacgggtgtt cgactttcgg gatgataatg aatcgaagcg ttcttgccat ttttcgggcc
      901 ctctcaggga tagcgtaaag gcagaacgct cagcatcagc ggcagcgctc ccggccgagc
      961 ctacttggcg cgcttccaga ccgtcaccgc cgttccgccg cccgatgcgg ccgggaccgt
     1021 gaacttcagt tcgtcaccga cgagggtgaa cggccacttc tgctcggacc cgtcccaatt
     1081 cgggaacgtg gcggcgtcga ccttcagggt gatggccttt tccgccgcgt tgacggacaa
     1141 ggtgccgaag tgggcaatgc tcccggcgac gatcgccttg ttttcctcgg gggtgccggt
     1201 ctcgcggtcg ttggaggcca gcttgggaag gccgacacgc atggccgcga tcgcatagcg
     1261 gccgttgtcg gtcacgatca ggatgccctt cggattgggg ccatatgttt ccgtcttcgt
     1321 cccgccctgc tcggtgaagg cggagaccac catccaggtg ccgacgaggt ccttggtagt
     1381 ctgcgcgctc accgcgccga ccgacatgac catccaggcg gccgacagga ccatcactcg
     1441 cgatgatctc ttcataattt ttgtttccgt tgctgaataa ctccagatga taccgtatct
     1501 aaccggccag ggccggcatt tcgtgatggg tcgagggttg ccgtcctgcg gtcaggcgcg
     1561 gcgcgagcgt ggattcagcg acgggtactc gctcgaaaaa ggcgctttaa atttcctata
     1621 ctccgacttc gccgcatatc agtcgaccga gcgattttcc ggcgcctact tgtcggtttc
     1681 ttattgtgga aagatttagc gtcttgtgcc gccgcaaaaa accgcaaccc ttgtcaattg
     1741 taaagctgga gcggcactga gcctcgccat acccgcagcg ccgatgtcat aaggggctag
     1801 cttatcgggc gtccgttgca accaaatgcc ccacgatgca caacaatgca ggactgattc
     1861 ccgcaaaact cccacaccga atcgcgttca aggccgccag caaaccatag cgcgggtgcg
     1921 cagacacgcc gtccggtctc tcgcagcctc atgaacccag gctcacgcag gccatgagga
     1981 ttgccacagc ccagccgcgc ggaggggtat gtctatgagc gccaccgtcc tgaagagaca
     2041 ccactgtatg gaatcgtcga gacttactac ccgcaatttc tcgcccgact cgaagccgaa
     2101 cgcggcccga tccacttcca catgctcttc ctcgacggcg tgtattcatt cgatcgtgca
     2161 cgaccggagt ttcaccgcgc gcgcggccga ccccggccga gctcgttcgg ctgttacaca
     2221 ccatcagcac acgcctcgcc cggttgctcg aacgacaggg gttgctcgtg cgcgatgccg
     2281 agagcgacta cctcgattta gagcccggtg aggcactcga ccagctgatc ggcgcttcca
     2341 tacgttcccg aatcgccatc ggccccaacg ccggtcgtaa ggcgctcact ctgcgcaccg
     2401 ttccggctca accggaacca ctcgcttcaa ccttgctcgc caagcagcct ggcttttcgc
     2461 tccacgcggc gacctgctgt gaggccaaac aacgcgataa gctggaaaaa ctctgccgct
     2521 atatcacccg acctgccatc gcgaacgaac ggcgctcgac caacgagagg ggacaagtca
     2581 tctatcggtt caaacagccc tttcgcggcg gcacaacgca cgtcgtgctc gatccgttcg
     2641 acttcatttc gcggctggcg gtcttggttc ctcgcccaag acacaagcac ccgagcaacc
     2701 acgggcaccc atgacctgga tgcagcgcct gaaacgggtc ttcggctgat tgcaagcaat
     2761 cagctaagcc gttcgcgaag cgaactgccg ccatcgacat agagacctgc tcgaaatgcg
     2821 gcggcaaact cagagtgatc gcctgcatcg gaggatcccg acgtcatcgc caccattctc
     2881 gagcacattc gtgcacgcga cgaagcccaa ccggcacaac cgcgggcgcc gccactgcgc
     2941 tcccagcacc ccgacacctc gaaccaggac aggctcttct cgtcacgacc gccgcacgct
     3001 cagcccacga agccctcccc aagcaaaaat caccaccgcc ttactttcag atcgcctggc
     3061 ttcgggcggt catacttcct atacccgtaa gttcctcgtt gatgatccgt gcatgtgcct
     3121 gactcaatcc gccgccgcca ccgcctttca gcgatcctgg aaagttcccg gctatccatt
     3181 cccgcgcttc ctgtcgaaac gccgtcaact catcgctcat gccactgtct cttgttgatc
     3241 tctgattgat tagaacccga acgcgtagcg caccgaggca ccccaccacc ggggattacc
     3301 tgcatagttc tcatacatcc cgaacccgcc ttcagccgga gaacctgcca ggtcgaacac
     3361 catcagccgg tggtcttcgt caaagagatt attgacaaac accgacacgt cccaatctcc
     3421 tttttcgctc atccagctca gccgtgcatt ggttatggcg tagccgctct ccttgccgac
     3481 gggtgagttc ttgagctgaa agtagtgtga actcatgtag tttacgtcac cctgaacggc
     3541 aagcatgccc cccagtgcag accactggta gcgaacaata ccactgatgt tcacctcggg
     3601 cgtcagcact gcagttcgat cgaccggcgt gccgtcaggc tgggtgtggc cgtcctcgac
     3661 ggtattgtcg atcaatccga gacccagcag cagatcgagg ccttctaccg gcgacgcctg
     3721 cagttcgagt tccagtccat aggtctcggc atcggtgttg aaaacgaccg ttgtcagact
     3781 gtccagcgca aatgcctggt agtcctgata gtcgtagtag taggcggcgc cattcaaccg
     3841 ggcaagcccg ccggcgaacg tccacttgaa gccgatctcg taggcgttca gcacttcttc
     3901 gtcgaaacgc atttcttcag gctcgcgcac gcctgcataa aagtcggttg catcgatcgg
     3961 tgcgttgaag ccgccggcct tgatgccgcg gttgtagctg atgtaggaga gcaggttgtc
     4021 cgagggcagc cactcgagcg caagcttggc tgtgatcagg tcatcgttca gtttggccag
     4081 gcccggatcg ccattgaccg ggtcgaaatc gaacgccggg ccgccgggtt cgttggtcac
     4141 ggcatccggg ctgaatgtcc agatggcgcc tcccagcccc aacccgtcct caacgtcgat
     4201 ttcgaacgag tccggcgctt cgaacaggga aaagaactgc tggaagtcca tgtccttttt
     4261 ttccttggac cagcgtaccc cgacggtgcc gcgcaacgtt tcggtgaagt cgtattcaac
     4321 ctgcgcaaac agcgccaccg attcggtgtt tgtactgaac ggattgttca atcccagggt
     4381 ctcaagcgac ggatcgttca gaccgaatcc ggggaaagcc gccgcaaaaa aattggatgc
     4441 agcaccgcca ttgaaaaact cgccctggat gtccaggtag taggcgccga cgacccagtt
     4501 gctggtgtcg gtgctgcccg caagacgcac ctcctgggaa aactgctcga tgtcggagcg
     4561 caacgtaaac gcaaagaagt cttccgggcc cgcatccgag tcctcgatgt agtccttgtc
     4621 gagttccgag taatcggtca cgaacacgag cgacatcgtg tccccaatac cgaactcgag
     4681 attgctggtg aagcccgtcg tctcgatctg attgaagcct tcgacgttga cggagccttt
     4741 caacagcttc tggtcgggat cctggtaacc ctccgtaatc ctcgaatccc cctcggcggt
     4801 aaggtcaggt ccatcgaagt cctcacccag tcccgtgatc gggttcaacc gtgcggagcg
     4861 atgagaaaac ggtgctacgt tcacgtcctg ctctccgcga cgcagattca gcaaccagct
     4921 cgtccgatcg ttgaagtcga tcagcacctg cccgcggatg gcccagtcgt cgccgtcgtt
     4981 cagattggaa ccgatccggt ttttgtatat gccgtcgtgg ttgttggtcg taaaggacag
     5041 gcgcccggaa attttgtccg tgatgccgcc gccgacggca ccttcgaact tgagctgatc
     5101 gtagtccccg taggtcccct tgatgtagcc atccagttcc tgcgagggct tgcgagtgat
     5161 gtagtgcacc agcccgccgg ttgcgttgcg cccgaacagg gttccctgcg gaccacgtaa
     5221 cacctccaca cgctcgaaat caaatagctg gaagccggca ccggaagctg cgctgatgta
     5281 gacctcgtcg acgtagacgg ccaccggcga ctcctggtga tcacctgaaa aatcgttctg
     5341 ggcaacgccc cggatgttgg tgaaaaacgc tgacggaccg ttcggctgaa tggtcgtcac
     5401 gcccggcgac atcgccgtga cctgctctgc cgtatcccag cccagttgat cgagctgatc
     5461 gccggtaaat gccgataccg caatgcccac atcctgcaga ctctgatcac gcttttgcgc
     5521 gaccaccaca atttcttcga ggaccgcggc ttgcgcactc ccgagccaga tcagaatcac
     5581 cagcgtcagc cagcggtagc cacacgtgtt tttgtacatc gcgctcattg cgctccctcc
     5641 ccggtacgtt ttccccgttg ctgaataact ccagatgata ccgaatctaa ccggccaggg
     5701 ccggcatttt ttgatgggtc gggggttgcg cattgctagg gtcccgttgt ggacaacggg
     5761 agtttagtcc agggctggcg cgtatatggg aaagagcttc atgtcgaaac gcttcagcgt
     5821 taagcgcatg cgcggcttca atgccggata ggtctcgacg atacgtcgga actccgcacg
     5881 gaaggtcgcg aaatacgcat ctgtcatctc tgagttgctg tgtgctgtct gcacgtcgct
     5941 gaatgtcttg acatacaact cagctatacc ctgcacacgc tcttcttctg tgggtgtcag
     6001 gtcagttagt tcggggtcac gacactttgc catgagggcc gacagatcgc tgttgcagac
     6061 aatcgcttcc tgaatttctc cgagtcggtt ggtcaccgct gctgatcgag tcacgtagct
     6121 cgaccgcgtg ttttgccgca cttgaagggc cagatacccc agcgtcgcta cgacggctat
     6181 tgccccgaaa aactctccgt atctccccag cattgcagcc aaagcctgat gatccatgac
     6241 ggtctcctct ccaatgaatg gaatgaggct acctcagtcc gtctgcgccg caagtcttct
     6301 gcgctaccat cccttcatct acaggaggag gtgagtcatg gatattcagg gtatcgctgc
     6361 gttgctgggc aactttgggg aattctttgg cgcaatagca gtggtcgcta cgcttgctta
     6421 tctggccgtc cagatcaggc aaaacaccaa cgcgcttcgt tcagccagtt atgagcattg
     6481 gaaccagcag gcagctgagt tcgcgcactt tcaggatcag cacgcaaaag agcttgccga
     6541 aaccgaagat ttggcgagct ttgatgaatt gaccggcgag cagttcaggt acgtcgcgtg
     6601 tctgctcaca attgctgcca atcaggcgca gtgtgcgttc cttcagcatc gggcaggaac
     6661 cgtcgacgac gacgtcttcg aggctcgaat aagagcgttc ctgttcttca tagagaagat
     6721 cccgcatctc aaagaaactt gggggctgat cactcgagag tccgaggttc ctgcctttgt
     6781 ggagtacatt gaggaacggg cgagctggct tcgaccagcg acggcgtgag ccgcctctga
     6841 cgccttcctc ttaacccgct tccgccaaag tcgtagtctc tcctcatcca cagggagagg
     6901 agaaacccca tggaacggct caatcagtgg cttggcttgg tcgccaactt cggcgtggtc
     6961 atcggcatcg tgtttctggc gtatgagatg cggctgaata cggactcaat gaaatccagt
     7021 acggcggcgc agagcatggc cgcgtggaat gaaatcgtct tagctgcggc caccaacccg
     7081 gatctgattg ccgagcagat agctgccaat cgggaaggga tcaggttgga tgctggaggg
     7141 ttacagatca gctacttcgc gggaagcatg ctgaagtcta cggagttcaa ctatctggag
     7201 cacgcccgag gaaacctcga gcaggagcaa tgggacgcct acgtctacgc gcagcgctgg
     7261 tttatgacgt acaacacttt cctgctccac gcctggagga tgcagcgata tacgtttgca
     7321 tcgagcttcc gtgaattcat ggacgacctg atccgcgaca tctgctcaaa gcaagagtgt
     7381 ccagagggtg gcaggccgga ggattggacg gagctgtcta cctagaacaa gcgaaaggct
     7441 tcttcgccgt cccaattcag aatagattgg gacgacgccc gctcgacaac atcaactgag
     7501 aacaggagat cctcaactta accagtggac caaaaaacat gggctggaca aaccttctcc
     7561 attcgagcgg cagaagcgtc cagagggatc cagatcccga gatagatccc agcgacaggc
     7621 gcgccatgtc gagagatgaa tcgattggtc gagacgccat cagcgaccgc ctctgaccgc
     7681 tgaggcgcac gggcaccggg atggaatacg aggatcaccg ccaggtcggt gccaaagaca
     7741 gcagagttct tctcatgtcg gattctcgat tactgcctga agaactcagg gcccgggagg
     7801 aacatcgggt tccccagcgt ggctttcagc ctcgccccct gtgttactta catcttcata
     7861 cagatacagc gactggccgg cgagggtggc ctcgttccaa cgggcgaagt cggcaggcga
     7921 catggctgac cattcgggag tctcctccac ctgggccctg ccgatgcgcc gccccacttc
     7981 caccagcgcg ttgcaatttt ccagccgctc ggctccgtac gccgcgagca gcgcatcgag
     8041 atcgtcgtgc tctcgagaca gcctttcgaa caccagggca tcctgcaatg ccttggtcgc
     8101 accactggcg gtatgcggtc tgaccacggc cgcggcatcc ccgaccagca ttacccgagc
     8161 ctcagaaaag tgcacggcca gctcgtcgta gatcggttga atggaaacct gctctctcgg
     8221 actgagccga atcaacgcag caatctgcgc cggcaaactg tcaccgagca gcgcgtcgaa
     8281 ctgggcatac atcgcctccg tcacggaacc ctcggggatc gacgagggct cgtgaagcac
     8341 cagaccatcg ggcggtcggg aatagattcc ccagttgatc cgccgctcgt cctgccccgg
     8401 cgtctcggct gcccccggaa tcatgtagag cacgctgtgc ccaccgtcgt aacaggcaga
     8461 caaccaactc tgccgctcat ccagcctcgc aatgacggcc cgatcggcaa cgcgggagtc
     8521 ttcgaaattt ccgcgccaca acacgtaacc cgcatagtcc gattcagatc cccaggtcat
     8581 ctgctcgcgg accagcgagc ggtaaccgtc tgccgccacg agaaggtccg cacgcctgcg
     8641 ctcgccgtcc gacgtttgaa aagagatgcc ctcggcactg tttgcgtaat cgatcaaacc
     8701 cagaccgttg tggtagcagg catcgggaat ggcagcgcgc aaagcgcgcc acagccggcc
     8761 ccagtggttg gcgacgccag atacgggttg ccgccagact acccgccctt cgaacgagcc
     8821 gtcgtgcacc acccaatcac gctgtcgaat cggacagccc gggtagtccg gcggcaggaa
     8881 gccccggtcg atgagttccg actggagttg attcgggagc acgatccctg ccccccggtc
     8941 ctgaagatcc gttggcgaac gctcataaac gtccacccgg caaccggcgc gtcggagcac
     9001 aatggcaatc gaacatcccg ccaggctgcc gccgatcacc gcaactttgg cctcttccag
     9061 cttcacaacc gctgcccttt gcccgccggc ggtaatggcg cgcaatgata cgggaattca
     9121 acgagcgtgt gtgaagccgc ccggctcaat gctttgctcg ctgcgcaggc cttccagcac
     9181 gatacggatc ttctcttcgg gattgtgttt gcgtcgtgtg gctcggcgaa tatcgcgcac
     9241 cgttttttcg acgctgtctt ggtttccttt cgtcatcatc gtttccttat cgggtaaacg
     9301 atgagccaaa agtgtctctt actcaatcag gacaatttgt ccaacagggg ctgacgggga
     9361 acactgtatg gcctccgtgt aagcgggatg ttcggcgaag atttgtgggt gatttacatc
     9421 acaatccggt ttcggcttta agtccatcat gcgctcgtgc tctcgcggcc cccccgaatc
     9481 tctccccccc acgcgagaca ccacaaaccc tggcacggac gccggggttt tttgtatcgt
     9541 cctggtgata caacccagat agataaactg tcgcgtaaca tataatgccg acctgcttcc
     9601 aggcggctat cgccctgaaa cccttatcac tgctggggtg taacttgact gctgatcagg
     9661 agatcgtcac cgcccgaggg accgtgggcg aaacgctccg cgtccctacg gccaacccgc
     9721 ggaacttctt cgaggccatc tccgaccccg gggccatgga acgcaccgag attctgggca
     9781 agctgttcct gccggccggg caggggccct ggccggtggt ggtgatcgtt cccggcagcc
     9841 tgggcattgc gccatcccac gttgccaagg ccgacctgct caacgaggcc ggcatcgctg
     9901 cctgtctgat cgatcctttc ggcacccgcg gggtcacctc caccgtcgcc aatcaggccc
     9961 aattttcgtt cgcggctagt gcctgggacg tgctggccag cgtagcagtc ctggccgcga
    10021 gagaggcgat cgacgctgcg cgcatcggcg cccagggaca cagtcgcggt ggctccgccg
    10081 tgctgtccgc ggcctgcatg gcgcggctcg agacggtcga tgtcaggtcc ggccttcgcg
    10141 cgatctacgc agcctacccc tggtcaggcc agcagtttct ctccccgttc gttggcgaca
    10201 ccttggtgcg cgccgtgatc ggagatcagg acgagtggtg tctggctcag caggtgcagg
    10261 ggcacattca ggcgataaga ctgtgtggag gagacgcgtc ctgccgcatt tttgccgggg
    10321 cccatcacag cttcgaccgg ggcacgccgc tggagctcat cgccgaggct tccgtctctc
    10381 caggagcccc taccgtctac atccgcgagg atggcgcgtt cgtgcacccg gccgatgggc
    10441 gtgctgaccc cgccttcacc gaccgtgagc tcatgatgta cggaatcaaa gccggctacg
    10501 ggcgcagggg cgcgcacctg ggcagcgagg gcgatcaggc cgatcggttt cacgaggaca
    10561 tgatggcctt ctggatcaag gcgttgggcg tcggctagtc ggggtcggcg tcttccatgt
    10621 acggctcggg atccaccccg agttcccggc agagccggcg gatcgccatc agctcgcggc
    10681 cgctcaagtg ctggtcggcc tgggccgtgt agcgggctat gcggatcagc agatctgcgg
    10741 cttcctcgtt accccgaaac cggccgatta cttccagggc ccagtcctgg ccttccccgg
    10801 aattggaccg catggcctgc acgaactgcc ggtgcaggtc gatggccttg cgcgggtcga
    10861 agacgttgag acgttcgatg ttttccagaa cccggtccac ggccaactgc tcgcttaggt
    10921 gtacgtgggc gtcggccatg gcgaccaggg cgctggccgc catggatgcc tccaggaatc
    10981 tgcggtcctt gaagcgtgcg aagccggtgc cgagaatctt gtctttcatg ctcatgtgga
    11041 gatccccgtc tgctgcgcct gcgaccggta ccccggttcc ggccgcctct caagcggaca
    11101 gcataaaggg cggggcggat tcgcgaaaca gccgcttgcg cggagcgcac gagaagcgct
    11161 ggatccggtt atgcttgcgg caggcggatc gaacttctgg ggggaacgaa atgcgtgaac
    11221 ttgatggaaa ggtggcggtg gtgaccggtg ctgccagcgg catcggccgg gcgacggcta
    11281 cgcgctttgc cggggaaggc atgaaggtgg tgatggccga catcgaggag gccgccctcg
    11341 agcgtgcggt ggccgagctt tcgggagccg ggcacgaggt tctcggcgtg cctacggacg
    11401 tctccgactg ggaagccgtc agcctgctgg ctgataagac catcgatgcg ttcggctcgg
    11461 tcaacgtggt gcacaacaac gccggcgtcg tcgtatccgg acccattgcc gatctcaccc
    11521 tggcggactg ggaatgggtt ctcggcgtcg acctgtggag cgtggtttac ggcgtcaagg
    11581 cctttctgcc gttgatccgg gaagcgggcg aggggcacat cgtgaatacc gcatccaccg
    11641 caggcctgca ggcctcggcg agcattgcgc cctacaacgt tgccaagttc ggcgtggttg
    11701 cgttgacaga aaccctgcgc gcggagctcg actccgaagg ctccgccatc tcagcttccg
    11761 tgctctgtcc cggcgccatc aatacccaga tcgtcaacag caaacgaaac cgccatagcg
    11821 cttcggcgca cgatcaccag acctcggatg cggagcgagc ttttgagagc cgggccggac
    11881 ggctgctggc ggaagagggc atggatcctg cggaggtagc cgacatggtg gtcaacgcca
    11941 tccggggaaa cgagttctgg atcgtcacgc acccccagtg gatcgacgtg ctcaagtcgc
    12001 gagtggacgc catggcccga gatcggtccc ttgccagggg ttttggaggt tgagccagcg
    12061 gtggcgccgg cctgcatagc gcacggttcg gccggcaacc cgggcgagat ggtgggcgcg
    12121 cgcagattcg aactgcggac ctctgccgtg tgaaagcagc actctaacca actgagctac
    12181 gcgccctgcg aagctagcct accaaaaacg gcggccgaag ctaccgcttc gccgctcggg
    12241 tgcagctggg aactgttcag agggggaagt aggcgcggga ttcgcccacc gcgtaggcgg
    12301 tgacctcttc gctgggcagc cccgcctcca gaacggctgc gcgcagcagc tcggggggct
    12361 cgagcagcgg ctcggcagtg aggtggaagg tgccccagtg cacggccatg gaccgcctcg
    12421 cgcggatgtc ctgatggatg cgcactgcct gctcagggtc ggcgtggtag ctgtgcatgt
    12481 acgcccgcgg ggcgtagctg ccgatgggta cgatgccgag gtcgaagccg tcgaaacgat
    12541 cgccgatggc cttgaactga acgtcgttgt atccggtatc cccgccaaac cagacggtaa
    12601 agtcgtccca ctccattgcc cagccggccc acagccgctt gtttcgatcg gtcagggtcc
    12661 gtcccgagac gtgctgggta ggggtggcgg tcaccgtcaa cgcggagccg ccgggctcga
    12721 ccgtctgctt ctcccaccag tccagctcgg tgactcgcga ttgatcgata ccggcatcca
    12781 ccagccactg cttcagtccc agaggaacga agtatcgggg ccggtcgccc agaaagcgga
    12841 tcgtaggctc gtcgaggtgg tcgtagtgat cgtgggagat gaccaccacg tcgatggggg
    12901 gaaggccctg ggcgtctacg cccggggccg acttacgacc cggccctgcg aacgcgacgg
    12961 gcgacgcgat tcgcgaaagt atggggtccg tgagcacgtt gacgccggcg tgctgaatca
    13021 gcacggtggc atgaccgacc caggtcaccc tggcgttgtt ggtctgctcg ccctcggcga
    13081 ccaggcgggg tgcagccgtg ggcactgcgc catcgcgatg ggcgtcgtag ctggaccacg
    13141 tgtcctgtcc taaaagtcgc cgtttaagga acgcaaaaac gcccccttgg gcttgttcca
    13201 cgtaggggtt ggtgaaacgg ccgctttcgg tgtggtgttc cggcgcgccc gccatggcgg
    13261 ggctcgcacc ggccagcgcg ccgatcagca cggccttacg cacggagcat ttgagcttca
    13321 gcatggggct agggtagcgc ctcagcggat cgac
//
LOCUS       DBNHLM010000188         8961 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_865240, whole genome shotgun sequence.
ACCESSION   DBNHLM010000188 DBNHLM010000000
VERSION     DBNHLM010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8961)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8961
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_865240"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="AC16QF_08655"
     CDS             <1..844
                     /locus_tag="AC16QF_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019752218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR6448669.1"
                     /translation="VSATPGPYEAERSGAVVEQVVRPTGLVDPDVIVRPASTQVDDLF
                     GEIRRTVENRERVLVTTLTKRMAEDLTEYLDEHGVRVRYLHSDIDTVERMEIIRDLRL
                     GEFDVLVGINLLREGLDMPEVSLVAILDADKEGFLRAERSLIQTIGRAARNVNGRAIL
                     YADHETGSMRKAIGETERRRAKQTAFNEAHGITPRTIEKQIADVMEGARAPAPGTVRR
                     KGAGPAHGSAERIEIPEDPKAFGRLLSELEERMLEHARNLEFEEAAQLRDELKKIRED
                     RLLA"
     gene            957..1361
                     /locus_tag="AC16QF_08660"
     CDS             957..1361
                     /locus_tag="AC16QF_08660"
                     /inference="COORDINATES: protein motif:HMM:NF013117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HIR6448670.1"
                     /translation="MLRSVGSFHPGDFIRVGENFGRVTEKGLVHTEIQSEDRDLITLP
                     NLYLMNQPVGVIRTSGTLISAEVTLGYDVHRHRLRDLLKSAAEAAELSGPFVQILELG
                     DHAIRYRVYGFLEDVANLVTKCRSRKTPTPSN"
     gene            1361..1543
                     /locus_tag="AC16QF_08665"
     CDS             1361..1543
                     /locus_tag="AC16QF_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448671.1"
                     /translation="MFDKAEFAARVNGLRVQRDELKAEIEALDMADGDARAQEVVWRE
                     RQIEQLQRIIDALENS"
     gene            complement(1607..2035)
                     /locus_tag="AC16QF_08670"
     CDS             complement(1607..2035)
                     /locus_tag="AC16QF_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6448672.1"
                     /translation="MKTIAEHNDWRGKANLDDGEWSTFVNPETSPDFFDHMTDVVKVK
                     FMGESWETGPWVVPNKFPPNTRADRHAHAADTIYVIMRGSMTFNDGTGWYEPGDVRWV
                     RADHTYGPEESGPDGCEFILISMGPIDVQWETGRTFHPDT"
     gene            complement(2163..2633)
                     /locus_tag="AC16QF_08675"
     CDS             complement(2163..2633)
                     /locus_tag="AC16QF_08675"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR6448673.1"
                     /translation="MKIADRCVVAFHYTLTDDDGQTLDSSSGREPLSYLHGNQGLIPG
                     LERELTGREAGDEFNVVVPPEEGYGVPNPALVQEVPLEVLAQIENLEVGTQLQSQSPD
                     GSAQIFVVEAVSDETATLNANHALAGRTLHFDVSIVDVRTATQEEIEHGHPHRS"
     gene            complement(2727..2987)
                     /locus_tag="AC16QF_08680"
     CDS             complement(2727..2987)
                     /locus_tag="AC16QF_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR6448674.1"
                     /translation="MKLYVGNLPWSTTDSDLEELFSTMGSVSSAQVITDRDTGRSRGF
                     GFVEMSNEDGTQAIAELNGHAMDGRQIKVNQANDKPRRTGGY"
     gene            3433..4077
                     /locus_tag="AC16QF_08685"
     CDS             3433..4077
                     /locus_tag="AC16QF_08685"
                     /inference="COORDINATES: protein motif:HMM:NF039189.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HIR6448675.1"
                     /translation="MDELIDFIRDALKAGASRRQVEKALRSAGWSNEQIAEGLDHFSE
                     TEFVIPVPRPELQGSARDAFLYLVMFTMLYVSAFNFGALLFDFINLAIPDPLDPLGDG
                     YRHFVRSSIRWETSALMVAFPIFLLIAAHINRAIAGDPSRRVSGVRRWLTYLTLAVMA
                     MILLGDVVALIYAWLNGELSLRMILKVTVVALIPAAIFAYYLWSMRADDEALKR"
     gene            4074..4484
                     /locus_tag="AC16QF_08690"
     CDS             4074..4484
                     /locus_tag="AC16QF_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448676.1"
                     /translation="MNVNRVAAITSSVLVAVVVVAGLIIAGSPGAQRLHRIDQLRVND
                     LRLISSVLNRYWNQHEALPESLGQLVDGRFLDELPRDPTTGDPYGYERTASDAVRLCG
                     MFERASVEESPGDYWYHVAGRYCFTLSFTPQKKT"
     gene            complement(4469..5731)
                     /locus_tag="AC16QF_08695"
     CDS             complement(4469..5731)
                     /locus_tag="AC16QF_08695"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HIR6448677.1"
                     /translation="MKRSSSPAEAQASGSCYVPPIELTVGQAQPIAANGGLSYMAFDH
                     DGDAGTGRATEDALRLIREGEGQAVIDLIENAPPGPIQTKWGLGFRRYAECLDFIREA
                     GIEAPEGGLAIPLRYTVHEEPSYSVVSSNALWDDPARTDDANALRKDERDSVRRCLYF
                     PQVLRDARRIGEYYPGLSPSSPECMDRLGVALGHCESRCENFYDAAEVERVFYPEMEK
                     LLLEFFPGATDALVYNHDVFDKSYDGDVVEDQDNKDPGVNTRYANLVHNDLNDNSGRV
                     RCRELLTRNLRNFGRERHYTEAEADAKMSRRFMSINLAKPMDTVRQNPFVLCAWPSFA
                     DQPYINNYRIYDDRVGETTRFTHRPDHEWYWFPHQQPNEVSMLKCYDSVTDGSVSRWS
                     FHTACIDPTAPADAPCRKNVVVRSYVFF"
     gene            5869..6387
                     /locus_tag="AC16QF_08700"
     CDS             5869..6387
                     /locus_tag="AC16QF_08700"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6448678.1"
                     /translation="MEPRVRQEIYGADPDTWVASPDAPFERATPVDGGYLFSDEWPYS
                     TGTDYCDWVILGGMVTAAGPETSPVPDVRMQDVFVPDYRVYEAPKVIEGVYAAKWRLR
                     HMLNELFERTSAGHPITPTERLRFRRNQVRATDRVFDTYAPLARMAGSAGIQEATGLE
                     RWWRDLQTAITS"
     gene            6351..6776
                     /locus_tag="AC16QF_08705"
     CDS             6351..6776
                     /locus_tag="AC16QF_08705"
                     /inference="COORDINATES: protein motif:HMM:NF039286.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448679.1"
                     /translation="MVEGSANRDHVLIQRQGGTIELYGSDVHTITWYTGPGVLLSRQE
                     FGETLIVELIANVPVEDGVSKVWHGTMVRAPNNPGTEIVREFRAETDVGALQAFSPDS
                     AVWRTKQPAIHVLQLPTDGPLAGRVAGTVSSTSTRVPWS"
     gene            complement(6897..7295)
                     /locus_tag="AC16QF_08710"
     CDS             complement(6897..7295)
                     /locus_tag="AC16QF_08710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR6448680.1"
                     /translation="MTISITSALDNDQVSLQLLKVLAENSFDSILITDTTKAGRVIYA
                     NKAFKKLAGYGQDEIVGKTPRELQGVGTSKEVIAELRAALSTGRKFEGRAINYKKDGT
                     PFIMHWRVLPVKLGGDIKAWIAIQRESSGS"
     gene            complement(7659..8237)
                     /locus_tag="AC16QF_08715"
     CDS             complement(7659..8237)
                     /locus_tag="AC16QF_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448681.1"
                     /translation="MDIQAPAQLPGNFGEFVGAAAVFLTLVYLLIQIRQNTHSMDENR
                     RLSTVQAQQDWTSMFNYAVTEFATSEHLPRAMAARLDSMHLQFEHGFLSREAYEATFG
                     RAIAQYGPMWRDVGAGLDVRRASFGMVIGIVFLAYEMRLNTNAMLSGTAAQAVTNWHV
                     IMMELATNRELVDAALQLNSRGLSFAQEDLRV"
     gene            8339..8827
                     /locus_tag="AC16QF_08720"
     CDS             8339..8827
                     /locus_tag="AC16QF_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448682.1"
                     /translation="MTIAELGSIGEFVGSFAVLATLIYLATQIKHSQKFAMAQAFQGR
                     TDLAVQLGGNLLQLDSDLLAKVAPTDLQSELFVDATKIDDLTREEQSRLRVAFYGMVY
                     ITDNSLYQVELGLMPDAHLESLAASSTLDYLGPCSRRLGVPLTPRVERALTQHEQASQ
                     AR"
BASE COUNT         1633 a         2654 c         2844 g         1830 t
ORIGIN      
        1 cgtctccgcc accccgggac cttacgaggc ggaacggtcc ggcgccgtcg tcgagcaggt
       61 ggtgcgtccg accggcctgg tggatccgga cgtgatcgtg cgccccgcgt cgacgcaggt
      121 tgacgacctg ttcggcgaaa ttcgccgcac ggttgagaac agggagcgcg tgctcgtcac
      181 caccctgacc aagcgaatgg cagaggatct caccgagtac ctggacgagc acggcgtccg
      241 ggtgcgctat ctgcactcgg acatcgatac ggtggaacgc atggaaatca tccgcgatct
      301 gcgcctcggc gagttcgatg ttctggtcgg catcaatctg cttcgggagg gcctggacat
      361 gcccgaggtc tccctcgtgg cgattctcga cgccgacaag gaaggctttc tgcgcgccga
      421 acgttcgctg attcaaacca tcggtcgcgc cgcgcgcaac gtcaatggcc gggcgattct
      481 ctacgccgat catgaaaccg gttccatgcg caaggccatc ggagagaccg aacgccggcg
      541 tgccaagcag acggcgttca acgaggctca cggcatcacg ccacgcacca tcgaaaagca
      601 gatcgccgac gtgatggagg gtgccagagc tcccgcgccc ggcacggtgc gccggaaagg
      661 cgccgggcct gcacacggat cggccgagcg catcgagatt ccagaggatc cgaaagcgtt
      721 tggcaggctg ctctcggagc tggaggagcg aatgctcgag cacgctcgca acctggaatt
      781 cgaggaggcc gcgcagctgc gcgacgagct gaaaaagatt cgcgaagacc ggctgctcgc
      841 ctgagacgac gccgttcgag cagactagaa tcggcaggat ggattcagaa ctccaacatc
      901 cgtgggtttt cggaaggggc cgtcacgccg ggcattgatc tcgaccggcg gggttcatgc
      961 tgcggtcggt cgggagtttt catccaggag acttcattcg cgttggcgag aatttcggac
     1021 gggtcaccga aaagggactt gtgcacacgg agatccagag cgaagaccgg gacctgataa
     1081 cgctgcccaa cttgtatcta atgaatcagc ccgtcggggt gatccgcact tcggggaccc
     1141 tgatatccgc ggaggtgacc ctgggctacg acgttcaccg gcacaggctg cgggacctgc
     1201 tgaaatccgc agcagaagcg gccgagctct ccggcccgtt cgtgcagatc cttgagcttg
     1261 gcgaccacgc gataaggtat cgggtttatg gcttcctgga ggacgtcgcg aacctcgtca
     1321 cgaagtgcag gagcaggaag actcccacgc cgagcaactg atgttcgaca aggctgaatt
     1381 tgccgcccgg gtcaacgggc tcagggttca gcgcgacgag ctgaaggcgg aaatcgaagc
     1441 cctcgacatg gctgacggcg acgctcgggc tcaggaagtt gtctggcgag agcggcagat
     1501 agagcagctg cagcgcatca tcgatgcgct ggaaaattcc tgaggcatac cggtgagttc
     1561 ccgattgaag tcgtaacctc ccccgcgttc gtgcgccgga ggggctttaa gtgtctggat
     1621 gaaacgtgcg tcccgtttcc cactggacgt cgatgggccc catggatatg agtatgaact
     1681 cgcaaccgtc cggtcctgac tcctcgggtc cgtaggtgtg atcagctcgc acccatcgga
     1741 cgtctccggg ctcgtaccat ccggtgccgt cgttgaacgt catgctcccc cgcatgatca
     1801 cgtagatggt gtctgccgca tgggcgtggc ggtcggcgcg ggtgttcggc gggaacttgt
     1861 tgggaaccac ccaggggccg gtctcccagg attcgcccat gaacttgacc ttcaccacgt
     1921 cggtcatgtg atcgaagaag tccggcgacg tctccggatt cacgaacgtc gaccactcgc
     1981 cgtcgtcgag gttggcttta ccccgccagt cgttgtgctc cgctatggtt ttcatgctct
     2041 ccccatcgtc tcggcggccc tcctggtgtg ggccccgtcc cggtagctaa gctgctcctc
     2101 gtatcttcag acgtgggcgc ggggttggca agacttcccg gacggcgggg cagagagcac
     2161 ggtcacgacc gatgcgggtg gccgtgctcg atctcttcct gggtcgcggt gcgaacgtcc
     2221 acgatgctca cgtcgaagtg cagggtacgt cctgcaagag cgtggttggc gttcagagtc
     2281 gcggtctcat cggagacggc ttccacgacg aagatttgcg ccgatccatc tgggctctgg
     2341 gactgcagct gcgtccccac ctcgagattc tcgatttggg ccagaacctc cagcgggact
     2401 tcctgaacga gggcagggtt cggaacgccg tagccttcct cgggcggaac gacgacgttg
     2461 aattcatcgc cagcctcccg gccggtcagc tcgcgctcca ggcccggaat caggccctgg
     2521 ttgccgtgaa ggtagctcag gggctctctt ccggaggagg agtccagagt ctggccgtca
     2581 tcgtcggtca gggtgtagtg gaaggcgacc acgcatcggt ctgcaatttt cataatcaac
     2641 ctctcgtcgg tcaggcacgc gttgcggcac ttgatggtgt caccggaacc cgccgcacga
     2701 cgggttccgg ctgcagctcg cctggctcaa tagccgccgg tacgccgcgg cttgtcgtta
     2761 gcctgattga ccttgatctg ccgcccgtcc atggcgtgtc cgttgagctc ggcaatcgcc
     2821 tgggtgccgt cttcgttcga catctcgacg aacccgaaac cgcgcgaacg gcccgtgtcc
     2881 cgatcggtga ttacctgtgc cgagctgacc gagcccatag tcgagaaaag ctcttccagg
     2941 tcgctgtcgg tcgtgctcca gggcaggttt cctacgtaca atttcactga ttgttctcct
     3001 tggcttgttg agccgtgtgc gtgcgtattc ccggatatga atcaattcga acgcggtttt
     3061 ttttccgctg accctttgtc agcggttgtc tgaggctaga agcgattgcc gatgcactgg
     3121 cgagaaatga agctcgggag aagatcggga cggggtttct aagcgcttgt catggtacac
     3181 acccgggcgg aggaaagctc gtactatttt ggagcgtcgg ctcgccgggc gagcggaggc
     3241 cgaacctttg gatctgcatg ggctcgccgt taacgggcct gtccgtcagc cgaactacgg
     3301 gatgtgtcac ggggggaact ctggcagcat acggatgccg aaacaggtga acggcatcga
     3361 atgccgtccg ggggtgcaac cccgagcaaa gtggggaagt ggtctgggcg acactcggtc
     3421 agggcaggag acgtggacga gctcatcgat ttcatacgcg acgccttaaa ggcgggcgca
     3481 tcccgcaggc aggtggagaa ggccctgcgg agcgccggtt ggtcgaacga gcagattgcg
     3541 gaaggcctgg accacttctc ggagaccgag ttcgtgattc cggtacccag gcccgagctg
     3601 caggggtcgg cgcgggatgc ctttctctac ctggtgatgt tcacgatgct ctacgtcagc
     3661 gcgttcaact tcggcgcgct tctcttcgac ttcatcaatc tggccatccc ggatccgctc
     3721 gaccctcttg gtgacggtta ccggcatttc gttcgcagca gtatccgctg ggagacgtcg
     3781 gcgctcatgg tggcctttcc gatctttctg ctgattgctg cgcacatcaa cagggccatt
     3841 gccggcgacc cctcacgaag agtgtcgggc gtgcgcaggt ggctcaccta cctcacgctc
     3901 gccgtcatgg ccatgatcct gctcggcgac gtcgtggcgc tgatctacgc gtggttgaac
     3961 ggagagctat cgctgcggat gattctgaag gtcacggtgg tggcgctgat cccggctgcc
     4021 atcttcgcct actacctctg gtccatgagg gcagacgacg aggcgctaaa acgatgaacg
     4081 tgaatcgcgt ggccgcaatc acgagttccg tcctggtggc cgtcgtggtc gttgccggtc
     4141 tcatcatcgc cgggtccccc ggcgcgcagc gcctgcaccg gatcgaccag ctacgcgtca
     4201 atgatctgcg gctgatcagc agcgtcctaa atcgttactg gaaccagcac gaggccctgc
     4261 cggagtcgtt gggccagctg gtggacggac gctttctcga cgagctgccg cgggacccca
     4321 ctacgggcga tccctacggg tacgagcgga cagcctcgga cgccgtcagg ctttgcggga
     4381 tgttcgaacg tgcgtccgtc gaggagtccc cgggcgatta ctggtatcac gttgccgggc
     4441 gttactgctt cacgctcagc ttcacgcctc agaaaaagac gtaggatcgc accaccacgt
     4501 ttttccggca gggcgcgtcg gcaggcgccg tcgggtcgat gcaggccgtg tggaacgacc
     4561 agcgcgacac ggagccgtcg gtgacggagt cgtagcactt gagcatggac acctcgttgg
     4621 gctgctggtg aggaaaccag taccactcgt gatccggacg gtgtgtgaag cgcgtggtct
     4681 cgcccacccg gtcgtcgtag atgcggtagt tgttgatgta gggctggtcg gcgaacgacg
     4741 gccacgcgca cagaacgaac gggttctggc gtaccgtgtc catgggcttt gccaggttga
     4801 tggacatgaa tcggcgcgac atcttcgcgt ccgcctcggc ttccgtgtag tgccgctcgc
     4861 ggccgaagtt gcgcaggttt ctggtgagca gctctcggca gcgcacccgg ccgctgttgt
     4921 cgttcagatc gttgtggacg aggttggcgt atctggtgtt cacgcccggg tccttgttgt
     4981 cctgatcctc caccacgtca ccgtcgtagg atttgtcgaa cacgtcgtgg ttgtagacca
     5041 gcgcatccgt ggcgccggga aagaactcga gcaggagctt ttccatctcc gggtagaaca
     5101 cccgctccac ctcggccgcg tcgtagaagt tctcgcagcg ggactcacag tggccgagcg
     5161 cgacgccgag ccgatccatg cactcggggc tggacggcga taagcctggg tagtactcgc
     5221 cgatcctgcg ggcatcgcgt agcacctggg ggaagtagag gcagcgccgc acgctgtcgc
     5281 gctcgtcttt tctcaaagca ttggcatcgt ccgtgcgtgc cgggtcatcc cagagcgcgt
     5341 tggaggacac caccgagtag gagggctcct cgtgtacggt gtagcgcagg gggatggcca
     5401 ggccgccctc gggcgcctcg atcccggcct cgcgaatgaa gtcgaggcac tccgcgtagc
     5461 gccgaaaccc caggccccac ttggtctgaa tgggccccgg gggcgcgttc tcgatcaggt
     5521 cgatgacggc ctgaccctcg ccctcacgga tcagccggag ggcatcctcc gtggccctgc
     5581 cggtgcccgc gtcgccgtcg tgatcgaagg ccatgtacga caggccgccg ttggcggcga
     5641 tgggctgcgc ctgccctacg gtgagctcaa tgggcgggac gtagcaggaa ccagacgctt
     5701 gcgcttcagc cgggctgctc gatcgcttca tgacagggcc ccgatcgcta aaatgaccat
     5761 cctggcacaa gggaccaacg cgggcaatcg agcccaaatc gcggcggcgc agctgctgcg
     5821 ggatggatcg ccggcgtcgt gggcgtgcat ccctgggaga tttccttcat ggagccgcgc
     5881 gtgcgacagg aaatctacgg cgccgatccc gatacctggg tggcgtcgcc cgatgcgcca
     5941 ttcgagcgcg cgacaccagt ggatggtgga tatctgtttt ccgacgagtg gccgtattcc
     6001 accggcaccg attactgcga ctgggtgatt cttggcggga tggtcacggc ggcgggccct
     6061 gaaacatcgc ccgtgcccga cgtgcgcatg caggacgtct tcgtccccga ctatcgggtt
     6121 tacgaagcac cgaaggtcat cgaaggggtt tatgcggcga aatggaggct gcgccatatg
     6181 ctcaacgaac tcttcgaacg tacgtccgct ggccacccga ttaccccgac cgagcggctg
     6241 cgctttcgcc ggaaccaggt tcgagctacg gatcgcgttt tcgacaccta cgcgccgttg
     6301 gcacggatgg cggggtccgc aggaattcag gaagcgacgg gcctggagcg atggtggagg
     6361 gatctgcaaa ccgcgatcac gtcctgatac agcgccaggg aggaaccatc gagctctacg
     6421 gatctgacgt ccacaccatc acctggtaca cggggcccgg ggtcctgctt tcgcgccagg
     6481 agttcggtga aacactgatc gttgagttga ttgccaacgt gcccgtggaa gacggcgttt
     6541 cgaaagtgtg gcacggcacg atggttcgcg cgccgaataa cccgggcaca gagatcgtcc
     6601 gcgagtttcg ggcggagaca gatgtcgggg ctttgcaggc attttcgccg gattccgccg
     6661 tctggcgcac caagcagccg gcgattcacg tgctgcaact gccgacggac gggccgttgg
     6721 cagggcgcgt cgctggtaca gtcagttcta cgtcgactcg ggttccgtgg agttgattcg
     6781 cgcggcgctc aacggcgttg tcgccgaaac gggcctgccg gcgccctcag ccgaatgcca
     6841 caacgtcagc gattgaaggg gtccagcaaa gcgtcagcgc ccggtatgtg cgacgactag
     6901 gagccggagg actcgcgctg aatggctatc caagccttga tgtcgccgcc gagttttacc
     6961 ggcagtacgc gccaatgcat gatgaacggc gtcccgtcct tcttgtagtt gatggccctg
     7021 ccctcaaatt tccgacccgt cgagagggcg gccctgagct cggcgataac ttctttgctg
     7081 gtgccgacac cctgaagttc tcgcggcgtc tttccgacga tttcatcctg cccgtaacct
     7141 gccagttttt tgaaggcttt gttcgcgtaa atgaccctgc cggccttagt ggtgtcagta
     7201 atcagaatcg agtcgaagct gttctctgcc agcaccttga ggagttggag gctgacttgg
     7261 tcgttatcca gcgcgctcgt gatcgaaatg gtcatggtct tccccttgcc tgtttgtcga
     7321 aagctacgac tgagctcgtg aaatcctcgt gaaacgagcg attgcgggcg ctgatgatgc
     7381 gagctgcgcc gcaggttgcc gtttgctttt ttgcctgcca gcgtcccgcg cggtcttcag
     7441 catgttctgg gcgtgcgggg tacccttcca catcacgtat atcgggtccg aagggttcgt
     7501 ggacggaccc tccaggatcg cttcgttcgg cacgtcgacc gtcagatggg gtccttcctt
     7561 cgccgagtga agaagtcgag ctgactcagc atgatctccg gcctgcgacg cccccagggg
     7621 tcctctggag gccgttgtga cgttggatca gttcttgttc agacccggag atcctcttga
     7681 gcgaaagata ggccgcgtga gttgagctgc agtgctgcat caaccaactc ccgattggtt
     7741 gctagctcca tcattatgac atgccagttc gtgactgctt gggctgccgt accagacagc
     7801 atggcgttag tattcagccg catctcatac gccagaaata cgataccgat gaccatgccg
     7861 aagctggcgc gccgaacatc gaggcctgca ccaacatctc tccacatggg cccatactgc
     7921 gcgattgccc ttccaaaggt ggcttcgtag gcctctctgc tgagaaatcc gtgctcaaac
     7981 tgcaggtgca tggagtccag tcgagccgcc atcgctcgtg gcaagtgctc cgaggtggca
     8041 aactccgtca cggcgtagtt gaacattgat gtccaatcct gctgcgcctg aactgtagac
     8101 agacgtcgat tttcgtccat ggaatgcgtg ttctgtcgta tctgaatgag cagatagaca
     8161 agtgtcagaa agacagcagc tgctccaacg aattcgccaa agttgcccgg cagctgagcc
     8221 ggagcttgaa tatccatacc tcaccgcctc ccgtagatgg agcgatggta gcgcagaaca
     8281 cttgcggcgc agaccggctg aggtagcctc gttccattca ctggagagga ggtttcagat
     8341 gacgattgca gaacttgggt cgattggtga gttcgttggg tcgtttgcgg tgttggcgac
     8401 gctcatttac ttagcaaccc agatcaagca tagccagaag tttgcaatgg cgcaggcgtt
     8461 tcaagggagg accgatcttg ctgtccagct gggaggcaat ctccttcagc tcgactcaga
     8521 cttgctggcg aaggttgcac caaccgacct tcaaagcgaa ctctttgttg atgccacgaa
     8581 aattgacgat ctgaccagag aggagcagtc ccggcttcgg gtcgcgtttt acgggatggt
     8641 gtatatcacg gataactccc tgtatcaggt cgagttaggg ctcatgccgg acgctcatct
     8701 cgagtctctt gcggcatcat caacgctcga ctatctaggt ccatgtagtc gacggttggg
     8761 cgttccgctg actccgagag ttgagcgggc cctaactcag cacgagcaag cctctcaagc
     8821 ccgttgatgg tggccttcga ctaaactccc gtcatccaca acgggagacg cccaatgggt
     8881 attcaggatc tcggggccat cggggagttc atcggctcgc tggtcatcat tttcacgctg
     8941 atggtgctga tctacgaagt g
//
LOCUS       DBNHLM010000189         2435 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1224958, whole genome shotgun sequence.
ACCESSION   DBNHLM010000189 DBNHLM010000000
VERSION     DBNHLM010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1224958"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="AC16QF_08725"
     CDS             complement(<1..471)
                     /locus_tag="AC16QF_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase domain-containing protein"
                     /protein_id="HIR6448683.1"
                     /translation="MTNPVFPDVRMNIVRAADPVTARVVSNELCMRGKSASFVKHLVL
                     DIGDTPLQGAFRAGQSFGVLAPGEDAQGKPHKVRLYSIACPTWGEDGEARLISTTPKR
                     LIDEFSPQTETDDPDDHHLFLGACSNYLCDLKAGDEVRLTGPNGKRFLLPADPDA"
     gene            complement(468..1286)
                     /locus_tag="AC16QF_08730"
     CDS             complement(468..1286)
                     /locus_tag="AC16QF_08730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6448684.1"
                     /translation="MSRIFLLISMLAVAACQPPSDPGGADAPSASVAPAPGAEVGALP
                     SLAERLADRPEADRARDAGRMPAEVVEFLGIEEGMTVLDVFSAGGWYAEVLSHAVGPA
                     GTVYAQNTEFLLSMRDGANEKAMAARVAVGRLPNVQRLDRELDDLGLAPGSVDAAMLA
                     LNFHDIYNRGGPDGALAFLGRIYSLLKPGGVLGLIDHHGAAGNDNAALHRVEKAKVLE
                     VIDASDFVLEGESDLLVNPDDDMTSMVFAPEIRGRTSRFLLRLRKPADATAVGP"
     gene            1422..>2435
                     /locus_tag="AC16QF_08735"
     CDS             1422..>2435
                     /locus_tag="AC16QF_08735"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6448685.1"
                     /translation="MPALDGLRILDMTQYEAGTACTQALAWLGADVVKIEAPGSGDPG
                     RGVWGETSEDSEYFVVWNSNKRSVAIDLRKPEGRDLLLEMLPHYDVFVENYGPGVIER
                     LNLDYDVMRAIHPEIIYARIKGFGLSGPWAEYKCYDMVGQAAASAFSITGEPDGPPMR
                     PGPTTGDAGTGVQMALAITAAYAQKLRTGEGQFIELSMQEAMTYYLRTATASTNYGER
                     PTPRTGNGDLATMSLYPCAGGGPNDYVFLMAVTPRMWGDMCVAIGREDLIEDPRYKRG
                     ADRAANHASLRAEIGRWTLERDKYEAMRILAEGGVPASAVLDTRDLFDNPHLLDRGFV
                     HTVE"
BASE COUNT          420 a          840 c          797 g          378 t
ORIGIN      
        1 cgcgtcagga tccgccggca gcaggaagcg cttgccgttg ggccccgtca ggcgcacctc
       61 gtcgccggct ttgaggtcgc agaggtaatt gctgcacgcg ccgagaaaca gatgatggtc
      121 gtccggatcg tccgtttcgg tctggggcga gaattcgtcg atgagccgtt tcggcgtcgt
      181 cgatatcagg cgggcttcac cgtcctcgcc ccaggtcggg caggcaatgg agtagagcct
      241 caccttatgg ggcttgccct gggcgtcctc accgggcgcg agcacgccaa aggactgccc
      301 ggcccgaaac gccccctgca gcggcgtgtc gccgatatcc agaaccaggt gcttgacgaa
      361 gctggccgac ttgccgcgca tgcagagctc gttggacacc acacgagccg tcaccgggtc
      421 tgctgcccga acgatgttca tccgcacgtc cggaaagaca gggttggtca tgggccgacc
      481 gccgtcgcat cagccggctt gcgcagccgc agcaggaaac ggctggtgcg gccgcgaatc
      541 tccggagcga agaccatgct ggtcatgtcg tcgtccgggt tgaccaggag gtcgctctcg
      601 ccttccagga cgaaatcgct ggcgtcgatg acctccagca ccttcgcctt ctccacccgg
      661 tgcagcgcgg cgttgtcgtt gccggccgcg ccgtggtggt cgatgagccc gagcaccccg
      721 ccgggcttca gcagcgagta gatccgtccg aggaaggcga gcgccccgtc cgggccgccg
      781 cggttgtaga tgtcgtgaaa gttcagcgcc agcatggccg catccacgct cccgggcgcg
      841 agcccaaggt cgtccagctc ccggtcgagc cgctgcacgt tgggcagccg gccgacggcc
      901 acccgcgccg ccatggcttt ttcgttcgca ccgtcgcgca tgctgagcag aaactcggtg
      961 ttctgggcat ataccgtgcc tgccggcccg acggcgtgag acagcacctc ggcataccag
     1021 cctccggcgg agaacacgtc gagcaccgtc atgccttcct cgatgccgag aaactcgacc
     1081 acctccgccg gcatgcgtcc ggcatcccgg gcgcgatcgg cctccggccg gtcggccaga
     1141 cgctccgcga gagacggtaa cgcgccgacc tcggcgccgg gagcgggcgc aacggacgcc
     1201 gaaggtgcat ctgcgccgcc gggatccgat ggcggctggc acgctgccac ggccagcatt
     1261 gaaatcagca agaagatgcg gctcatgaac tcctccctct gcctcagtgc gacccagcat
     1321 agcgcgctgc gggctccccc tgtagcccga cggccgcttt cttcatttcg gtgctgacca
     1381 ccgatatgct tgtcggtggg gaacacacaa ggggaaagcc catgccggct ctggacggtt
     1441 tgcgaattct ggacatgacc cagtacgagg cgggcactgc ctgcacccag gcgctcgcct
     1501 ggctgggcgc cgacgtggtg aagatcgagg cgccaggctc cggcgacccc ggccgcggcg
     1561 tctggggaga aacctcggaa gattccgagt acttcgtggt gtggaacagc aacaagcgca
     1621 gcgtcgccat cgacctgcgc aaacccgagg gccgcgacct gctgctcgag atgctgcccc
     1681 attacgacgt gttcgtcgaa aactacggtc ccggcgtgat cgagcgcctg aacctggatt
     1741 acgacgtgat gcgcgccatt caccccgaga tcatctacgc ccgcatcaag gggttcggcc
     1801 tcagcggccc gtgggcggag tacaagtgct acgacatggt gggccaggcg gccgccagcg
     1861 cgttttccat caccggcgag ccggacggtc ctccgatgcg gccgggcccc accacgggcg
     1921 acgcgggtac cggcgttcag atggccctgg ccatcaccgc cgcctacgcg cagaagctgc
     1981 gcacgggaga aggccagttc atcgagctgt ccatgcagga ggccatgacc tactacctgc
     2041 gcacggccac ggccagcacg aactacggcg agcgcccaac cccgcgaacg ggcaacggcg
     2101 atctggcaac catgtccctg tacccctgcg ccggcggggg ccccaacgac tacgtcttcc
     2161 tgatggcggt gacgccaagg atgtggggcg acatgtgcgt agccataggc cgcgaggatc
     2221 tcatcgagga tccccgctac aaacgcggcg cggatcgtgc ggccaaccac gcatcgctgc
     2281 gcgcagagat cggcaggtgg acgctcgagc gcgacaagta cgaggccatg cgcatcctcg
     2341 ccgagggcgg cgtgcccgca agcgccgtgc tcgacacccg ggacctgttc gacaaccccc
     2401 atctcctgga ccgcggcttc gtgcacaccg tggag
//
LOCUS       DBNHLM010000190         4432 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_207075, whole genome shotgun sequence.
ACCESSION   DBNHLM010000190 DBNHLM010000000
VERSION     DBNHLM010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_207075"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="AC16QF_08740"
     CDS             <1..506
                     /locus_tag="AC16QF_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6448686.1"
                     /translation="PIPQVPEGTTARDFYADIVKTQFSERFGHDFSTMSTAQTLDSIE
                     YFVFPNMFLFPGLSLPMVYRFRPDPDDPDFCTFDLLFLRPKPKDRPAPPPPDPVDLDV
                     DDSYMLVEGTGRLGGVYDEDTVNLAAQTRGFKSSLKRGETLGNYQEIRARHLHRMVNR
                     YISGELP"
     gene            complement(613..1362)
                     /locus_tag="AC16QF_08745"
     CDS             complement(613..1362)
                     /locus_tag="AC16QF_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448687.1"
                     /translation="MRKILTSIAAATLLFGISLSAAAAPGWLATGLTVMPENAPKVVA
                     ALDALFDSKVGRKLPGRVILRANLADGNNPETHAIVSLFRSAAESEAYRAELWADPAW
                     GEFMAAMRGLNETAGSTMRGMIAYNSGERADSDVVWVNHYVTVSEPAVLLSAMRSYSQ
                     SATGQAAPGQVHLSAVTAGGVGSASHIISVGWASEAEWESEVQRTAGNPQWNALIRTM
                     RAVAEYHGATLQRDIKSWGKASVEEITNLGN"
     gene            complement(1513..1950)
                     /locus_tag="AC16QF_08750"
     CDS             complement(1513..1950)
                     /locus_tag="AC16QF_08750"
                     /inference="COORDINATES: protein motif:HMM:NF018717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR6448688.1"
                     /translation="MEVINQLYPTRKDQIEKMMEPGPDGPIVMVNLLKFREKAEYEDG
                     RETDLTGREAYMLYGAAVSKLITEYGGRLVFAGDVTNLTIGEVEELWDEIALAEYPNR
                     AALVAMSTSEAYAKIAAHRTAGLAGQLNIETTYQAGLTQPLRG"
     gene            2192..3670
                     /locus_tag="AC16QF_08755"
     CDS             2192..3670
                     /locus_tag="AC16QF_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448689.1"
                     /translation="MLERIRNLSLLRVATIYVVGGWIVIQFAQIAFEAFEVPNFVLQW
                     VMAIVFSGFPLVILALSMMGVPFRRSAAEEVEERDESREESAQPQQPVDPGDLPARDN
                     RPSIALLPFRYLGEGEAFLAEGLRMGLQTNMVRLSGLFQVYSLLNEAYVSGEVDGEVR
                     GRDANAQYVLEATVHELTGRVRISLQLTEVATGRCAWAEQYDRDLEHLFELQDEIMKE
                     VIASLHIRLRNAGIEDSWFDQLRSAVAKEWYFRGANHLAGMSRETNAAAREAFEKLHE
                     LEPESAIGSSNVALTYWNDFAAGWGPDRDEALAHAIEWAKLARSYDRNDGIGDAVYAY
                     EQLGRRNYEGAMEAARRSVRVRPNCPISLGVLAQVENCSGNHDAAAGLAEIAQSKVEV
                     PFPWVCNVLATAQRDGGQLERSLLTLERALQAAPDDVDLLVTKCSDHALLSDLESAES
                     AASRILSVEPEWSTAQYAAEALYRSPDELDRVVAALRSAGLP"
     gene            3787..>4432
                     /locus_tag="AC16QF_08760"
     CDS             3787..>4432
                     /locus_tag="AC16QF_08760"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR6448690.1"
                     /translation="MEGLETPWSMAWLPDGRMLITERRGELLRFDPEDESLTEIEGVP
                     AVRAEGQGGLFDVVLHPRYAETGWVYLAYAYLDAESGRSSTAILRARLEGDALIDQEP
                     LYRAEQDFGVKSELKQWVKQLLGRQRPGDSRAHYGGRMVFDEAGYLYFSVGDRGSRDL
                     FPQDLTRDAGKIYRIHDDGRIPEDNPFLHREDAKPAIYAYGLRNPQGMARHPGTG"
BASE COUNT          753 a         1359 c         1454 g          866 t
ORIGIN      
        1 aaccaatacc acaggttccg gagggaacga ccgcgcggga tttctacgcg gacatcgtca
       61 agacccagtt ctccgagcgc ttcgggcacg acttctcgac catgtcgacg gcgcagacgc
      121 tcgattccat cgagtacttc gttttcccga acatgtttct gttcccgggg ctatcgctgc
      181 cgatggtgta ccggttccgg ccggacccgg acgacccgga cttctgcacg ttcgatcttc
      241 tgtttctgcg gcctaagccc aaggatcggc ccgctccgcc gccgcctgat ccggtggacc
      301 tggacgtgga cgacagctac atgctggtgg aggggacggg acggctgggc ggcgtgtacg
      361 acgaggacac cgttaatctg gcagcccaga cccgcggctt caagtcgagc ctcaagcgcg
      421 gcgagaccct gggcaactac caggagattc gcgcgcgcca cctgcaccgc atggtcaacc
      481 ggtacatcag cggcgagctg ccctgacaag ggcaacgtcg ccagagacgt acgccgacca
      541 acggcgccgt gacgtagacg ttactcagac gaaggcaggc gcccggcatg agccgggcgc
      601 cgaaggcgga cttcagttac cgagattggt gatctcttcg acagacgctt tgccccagga
      661 cttgatgtcc cgctgcaggg tggcgccgtg gtactcggcc actgcccgca tggttctgat
      721 tagcgcattc cattgaggat tcccggcggt gcgctggacc tcgctctccc attcagcctc
      781 gcttgcccag ccgacggaga tgatgtggga agccgacccc accccgccgg cagtgaccgc
      841 gctgagatgc acctgacccg gagcggcctg gccggttgcc gactggctgt agctgcgcat
      901 ggcgctcagc agaacggccg gctcggaaac cgtcacgtag tgattcaccc agacgacgtc
      961 ggagtctgct cgctcgccgc tgttgtaggc gatcatgccc cgcattgtgc tgcctgcggt
     1021 ctcgttcagc ccgcgcatcg cggccatgaa ctccccccag gcggggtcgg cccagagctc
     1081 ggcgcgatag gcctcgctct cagccgctga tcggaagagc gatacgatgg cgtgggtttc
     1141 cggattgttc ccgtctgcca ggtttgcccg caggatcacg cggccgggca gcttgcgtcc
     1201 gaccttggag tcgaacagcg catccagggc cgccaccacc ttgggggcgt tctcgggcat
     1261 gaccgtcagc ccggtggcga gccatcccgg tgcggcggcc gcgctgaggc tgattccgaa
     1321 cagcagagtt gctgcggcga ttgaagtcag gatctttctc atggttagta ttttccttta
     1381 ttgatctgcc ccttcccgag gcaggtgccg gtcactatag cgaacgagag cgacgtttgg
     1441 cccgtctttt cgcccctggg ccggggtatc cgttccccga agcgtccgtt gccctgcatg
     1501 catgcgccag ggctaccccc gcagtggctg ggtaaggccc gcctggtaag tcgtctcgat
     1561 gttgagctgg cccgccaggc cggcggtgcg gtgggcggcg atcttcgcgt aggcctcgga
     1621 ggtggacatg gccaccaggg cggcgcgatt ggggtactcc gccagggcga tctcgtccca
     1681 cagctcctcc acctcgccga tggtcaggtt ggtcacgtcc cctgcgaaga cgagccggcc
     1741 accgtactcg gtgatgagct tcgataccgc cgcgccgtag agcatgtagg cctcgcgtcc
     1801 ggtcaggtcc gtttcgcggc cgtcttcgta ctccgccttt tcccggaact tcagcaggtt
     1861 gaccatgacg atgggaccat cgggccccgg ctccatcatc ttctcgatct ggtcctttcg
     1921 ggtggggtag agctgattga tcacttccat atcgggttcc tctggctgat accctcgggc
     1981 tccatagcga ccgcggggcg cgtttaaata atgataatca ttacacgatg tcacgcgcaa
     2041 ccgagccggt ttcccgggtc tgaacgtgct ggctgaaggg gcgccgtgcc ggcgtcgcgt
     2101 cgtggcgtat tctgcggtag ccttcggatt cggaacgctg ctgaaatcca ctcccggtct
     2161 tgccccctaa tttcttgaac gtcatgacga agtgctagag cgaatccgga atctcagtct
     2221 gctgcgggtc gcgacgatct acgtggtggg cggctggatc gtcatccagt ttgcacagat
     2281 cgcgtttgaa gccttcgagg tgcctaattt cgtcctgcaa tgggtcatgg cgatcgtgtt
     2341 ttcaggattt cccctggtga ttctcgccct gagcatgatg ggcgtgccct ttcgccggtc
     2401 cgccgccgag gaagtcgagg agcgtgacga gtcccgggaa gagagcgcac agcctcagca
     2461 gccggtggat cctggagacc ttccggcgcg cgacaatcga ccgtcgattg ccctgctgcc
     2521 attcaggtac ttgggcgaag gggaagcgtt cctcgccgaa ggcctgcgca tggggctgca
     2581 gaccaacatg gtacgcctct ccggtctgtt tcaggtttac agccttctga acgaggccta
     2641 tgtcagcggc gaggtcgatg gcgaggtcag agggcgcgat gccaacgcgc aatacgttct
     2701 ggaagcgacg gttcacgagc tgaccgggcg agtgcgaata tccctgcagc tcaccgaagt
     2761 tgcgacgggc aggtgtgcgt gggcagagca gtatgatcgc gatttagaac atctcttcga
     2821 gctgcaggat gagatcatga aggaggtgat cgcctcactg cacatccgtc tgcgcaatgc
     2881 cggcatcgaa gactcgtggt ttgaccagct tcgaagcgcg gtagccaagg agtggtattt
     2941 ccgtggtgcg aaccatctgg ctggtatgtc ccgcgagacc aacgcggcgg cccgcgaagc
     3001 gttcgagaag ctgcacgagc tcgagccgga gtccgccatc ggctcgagca acgttgcgct
     3061 cacctactgg aacgactttg ctgccggttg gggcccggat cgggacgagg ccctggccca
     3121 cgcgatcgag tgggccaagc ttgcgaggtc ttacgatcgc aatgacggga tcggggatgc
     3181 cgtgtacgcc tacgagcagc ttggtcggcg aaattacgag ggggccatgg aagcggcgcg
     3241 ccgcagcgtc agagtccggc ccaactgccc gatctccctg ggcgtgctcg ctcaggtcga
     3301 gaactgcagc ggcaatcacg atgcggctgc cggtctggcg gagattgccc aatccaaggt
     3361 ggaggtgcct ttcccatggg tatgtaacgt cctggccacg gctcagaggg acggtggtca
     3421 gctcgaacgt tctttgctga ccctcgagcg cgctttgcag gccgcgcccg atgacgttga
     3481 cctgcttgtc acgaagtgca gtgaccatgc gctgctgtct gatctcgagt ccgctgaatc
     3541 cgctgcaagc aggattctct cggtggaacc cgagtggtcg acggcccaat acgcagcaga
     3601 agcgctctac agatcgcccg atgagctcga tcgggtggtt gcggcgctgc gatctgcggg
     3661 tttgccatga ggggccgttt tggaggttgg ttcgaagctc ggcctttccg gagggccgtc
     3721 tggggcctgc ttctgtggtt gtgcgtcggc ctttcccacg cgggaaccta tgaggtggag
     3781 accgtcgtgg aaggtctgga aacgccctgg agcatggcct ggctgccgga cggccgcatg
     3841 ctcatcacgg agcgccgggg tgagctgctg cgcttcgacc ccgaagacga atcgcttacc
     3901 gagatcgagg gtgttcccgc ggtgcgggcc gagggtcagg gcgggctctt cgacgtggtg
     3961 ctccatccgc gctacgcgga gaccggctgg gtctacctgg catacgccta cctcgatgcg
     4021 gagtcgggca gaagcagcac ggcgatcctt cgggcgcggc tcgagggaga tgcgctgata
     4081 gaccaggaac ccctttaccg cgcggagcag gacttcggcg tgaagtcgga acttaagcag
     4141 tgggtcaagc aactcctggg gaggcaacgc ccaggcgatt cgagagccca ctacggcggc
     4201 cgcatggtct tcgatgaagc gggatatctg tacttctccg tgggagaccg gggcagtcgg
     4261 gacctgtttc cccaggacct gacccgtgat gcgggtaaga tctaccggat ccacgacgat
     4321 ggccggatcc ccgaggacaa cccgttcctg catcgggagg atgcgaagcc ggccatctac
     4381 gcctacgggc ttcgcaatcc ccagggcatg gcccgccatc ccgggacggg ga
//
LOCUS       DBNHLM010000191         2725 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1463875, whole genome shotgun sequence.
ACCESSION   DBNHLM010000191 DBNHLM010000000
VERSION     DBNHLM010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1463875"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="AC16QF_08765"
     CDS             <1..1085
                     /locus_tag="AC16QF_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HIR6448691.1"
                     /translation="QSVVIDVGSAPALEFTAYGPDGMAGLTRSQVVDNLVASGLWTAL
                     RTRPFSKVPSPDSEPHSLFVTAIDTNPLAADPGVVIPEYATEFAAGLDVLAHLTSGAV
                     HVCQESGAFLPAGNDPKLSVQEFAGIHPAGLPGTHIHFLDPVGPSKTVWFIGYQDVAA
                     VGRLFLTGEIMIDRVVALGGPGVEKPRLLKTVLGVNLEELTAGELAEGDQRIISGSVL
                     SGRACAGPVGYLGRYHLQVSVVPEDKERRLFGYLTPGLKKHSVFPIYLSKWLGGADVE
                     FSTTTNGSPRGMVPIGTFESVMPMDVLPTQLLRALLVGDLETAISLGCLELDEDDIAL
                     CTYVCPGKYEYGPVLREVLTQIEKEG"
     gene            1086..2270
                     /locus_tag="AC16QF_08770"
     CDS             1086..2270
                     /locus_tag="AC16QF_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HIR6448692.1"
                     /translation="MSLRSFLDDVAPHFEKGGRFAWAFPAYEAVDTALYTPGRVTGGP
                     SHVRDGLDLKRIMITVWLCAFIPVFAGSYVVGLQANEVMQSLGIESVDNWRGLFLDNL
                     TSYDPTSIWDCVLHGLAYFLPLYVTVFVAGIVFEIWFASVRGHEVNEGFFVTSILFTL
                     TLPATTPLWMAAVGIVFGVVIGKEVFGGTGKNFLNPALTGRAFLYFAYPAQMSGDAVW
                     VAVDGYTRATALGNAALGESYAVDWLAAFLGNMAGSLGETSTLAILLAGVILVASRIA
                     SWRIMAGVMAGMVAMSVVLNLIESNNPMFAMPFYWHFVLGGFAFGMVFMATDPVSAAM
                     TNAGRWIYGGLIGVMCVLIRVVNPAFPEGMMLAILFGNLFAPLIDHFVVQRNVNRRLA
                     RA"
     gene            2263..>2725
                     /locus_tag="AC16QF_08775"
     CDS             2263..>2725
                     /locus_tag="AC16QF_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HIR6448693.1"
                     /translation="MPDFDKEGLRNVLLVAVSVCLVCSVIVSSAAVILKPRQLINQEL
                     DEKQNILRAAGMLPQGSNQDMQGRGIDELFDQFTVHAVDLETGQFAELDEIDAYDPIK
                     AAKQASRSRELSADEDIATIGRRENVSLVYLRRDSDGVLDKVVIPVRGYGLW"
BASE COUNT          444 a          852 c          863 g          566 t
ORIGIN      
        1 tgcagtccgt cgtgatcgat gtagggtccg ccccggccct cgagttcact gcttacggcc
       61 ccgacggaat ggctgggctc acccgctctc aggtagtgga caatctcgtc gcgagcgggt
      121 tgtggacggc cctccggacc aggccgttct ccaaggtccc cagcccggat tccgagcccc
      181 actccctttt cgtgaccgcc atcgacacca acccgctggc ggcggatccc ggggtggtga
      241 tccccgagta cgccacggag ttcgctgcag gcctcgatgt cctggcgcac ctcacctctg
      301 gcgccgtgca tgtctgtcag gagtcgggcg cgtttctgcc agccggcaac gaccctaagc
      361 tcagcgtcca ggaattcgcg ggcatccatc ccgctggcct tcccggcact cacattcatt
      421 ttctggatcc ggtcggaccg tcgaaaaccg tctggttcat cggctaccag gacgtcgctg
      481 cggtgggcag gctgttcctg accggtgaga tcatgatcga tcgcgtggtg gccctgggtg
      541 ggccgggcgt cgagaagccg cggctgctca aaaccgtgct cggcgtaaat ctcgaggaac
      601 tcacggccgg cgagctcgcc gagggcgacc agcgcatcat ctccggttcc gtgctttccg
      661 ggcgggcctg cgccggtccg gtggggtacc tcggtcgtta ccaccttcag gtgtcggtag
      721 taccggaaga caaggagcgc cggcttttcg gttacctcac gccgggtttg aaaaagcact
      781 ccgtttttcc catctacctg tcgaagtggc tcggcggcgc agacgtggaa ttctccacca
      841 ccacgaacgg cagcccgcgg ggcatggttc ccatcggtac cttcgaatcc gtaatgccga
      901 tggacgtgct gccgacgcaa ctgcttcgcg ctctgctcgt aggcgatctg gaaacggcca
      961 tcagtctggg ctgtctggaa ctcgacgagg acgacatcgc gctgtgcacg tacgtgtgtc
     1021 ccggcaaata cgagtacggt cccgttcttc gcgaagtgct cacgcagatc gagaaggagg
     1081 gctagatgag cttgcgatct ttcctcgacg atgttgcccc acacttcgag aaaggcggcc
     1141 ggttcgcctg ggcatttccg gcctacgagg ccgtcgacac ggctctgtat acccctggga
     1201 gagtgacggg cggcccctcc catgtccgtg acgggctgga tctcaagcgg atcatgatca
     1261 cggtgtggct gtgcgcgttt attccggttt ttgccggtag ctacgtcgtg gggctgcagg
     1321 ccaacgaggt gatgcagtcc ctggggatcg agtccgtgga caactggcgc ggcctcttcc
     1381 tcgacaatct gacgagctac gatccgacca gcatctggga ctgcgtcctg catggcctgg
     1441 cgtacttcct gcctctgtac gtgaccgtct tcgtggcggg aatcgtgttc gagatctggt
     1501 tcgcctccgt tcgcgggcac gaggtcaacg aaggcttctt cgtcacgagc atactgttca
     1561 ccctgacgtt gcccgccacg acgccgctat ggatggccgc cgtcgggatc gttttcggag
     1621 tggtcatcgg caaggaggta ttcggtggca ccggcaagaa cttcctgaat cccgctctca
     1681 cggggcgggc gttcctttac ttcgcctacc cggcgcagat gtccggtgat gcggtgtggg
     1741 ttgccgtaga cgggtacacg cgggcgaccg cgctgggcaa cgcggccctg ggagaatctt
     1801 acgcagtgga ctggctcgcc gccttcctcg gcaacatggc gggctccctg ggcgagacat
     1861 cgacccttgc gattctgctt gccggggtca ttctcgtggc gtcccgaatt gcctcctggc
     1921 gcatcatggc cggcgtgatg gcgggaatgg tggccatgtc cgtcgtcttg aacctgatcg
     1981 aatcgaacaa ccccatgttc gccatgccgt tctactggca cttcgtgctc gggggcttcg
     2041 ccttcggcat ggtgttcatg gccacggacc cggtctccgc cgcgatgacc aatgcagggc
     2101 ggtggattta cggtgggctc atcggggtga tgtgcgtgct catccgcgtg gtcaacccgg
     2161 ctttcccgga aggcatgatg ctcgccatcc tctttggcaa cctatttgcc ccgctcatcg
     2221 accatttcgt agtgcagcgc aacgtcaacc ggagactggc tcgtgcctga tttcgacaag
     2281 gaaggactgc gaaacgtgct gctggtggcg gtatcggtct gcctggtgtg ctcggtgatc
     2341 gtgtcctcgg cggccgtgat cctcaaaccc cggcagctga tcaaccagga actcgacgag
     2401 aaacagaaca tcctgcgggc cgccggcatg ctgccgcagg gcagcaatca ggatatgcag
     2461 ggccgcggca tcgacgagct tttcgatcag ttcaccgtgc atgccgtgga tctcgagacc
     2521 ggtcagttcg ctgagctcga cgagatcgac gcatacgacc ccatcaaggc cgcaaagcag
     2581 gcgagccgct cgcgtgagct gtccgcagac gaggacatcg caaccatcgg ccggcgcgag
     2641 aacgtctctc tcgtctacct gcgtcgagac tcggacggcg tgttggacaa ggtggtgatc
     2701 ccggttcgcg gatacggact gtggg
//
LOCUS       DBNHLM010000192         7091 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_626170, whole genome shotgun sequence.
ACCESSION   DBNHLM010000192 DBNHLM010000000
VERSION     DBNHLM010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7091)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7091)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7091
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_626170"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="AC16QF_08780"
     CDS             complement(<1..189)
                     /locus_tag="AC16QF_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6448694.1"
                     /translation="MPGESCRVGYRHAMDAVPGSVWRTLADFGSLLSWVAGGDEGRIE
                     LSGEGIGMTRDLWLPSVGR"
     gene            462..2054
                     /locus_tag="AC16QF_08785"
     CDS             462..2054
                     /locus_tag="AC16QF_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019689397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6448695.1"
                     /translation="MNTKPLDAIVVGAGFAGLYMLHRLRGLGLKTRVFEAGSDIGGTW
                     FWNRYPGARCDIESMEYSYQFSDELAREWEWSERYATQPEILGYINHVAERFDLRGDI
                     QLNTRVERARFSEETNRWTVTTSDGGEHEAKYLVMATGCLSSTNLPEFDGIDSFQGDW
                     YHTGQWPHEGVDFTGKRVAVIGTGSSAIQSIPLIAEQAEQLTVFQRSPNFSVPAHNRA
                     LDPEAVARIKARYPEFRDANRQTAFHADFRYNESNALDVSAEQREREYEARWERGGLP
                     FLAAFADLVFDADANETAAEFIRGKIRETVKDPDTAELLCPTSVVGCKRLCVDTDYYA
                     TFNRENVTLVSVRDTPIEAITPKGLRTADEEYEFDAIVFATGFDAMTGALMKMDIRGI
                     GGRSMQDAWSEGPKTYLGLAVSGFPNLFTVTGPGSPSVLTNMLPTIEHHVDWIADCVA
                     YMEHEGMSRIDAMPEAQTAWVAHVNEVGDSSLYPRCNSWYLGANIPGKPRVFMPYLGF
                     PPYAEKCREVAESGYDGFALSA"
     gene            complement(2074..3255)
                     /locus_tag="AC16QF_08790"
     CDS             complement(2074..3255)
                     /locus_tag="AC16QF_08790"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6448696.1"
                     /translation="MQVSRALIVGGGIGGLTAALCLARQGIAVSVFEQTADLAEVGAG
                     IQLSPNASRVLHHLGLEGALGSVAFLPRAVEMRDWRSGRLLASSPIDEAFRDRFVYPY
                     YHVHRADLLDVLERALRRAPGIELRTGARVEGIEPGDSAVFVEAGGERHGGDLLIGAD
                     GIHSTVREALFGAEAPRFTGNVAWRGLVPAGAVPSGFVRPAASAWWGPHKHFVHYYVR
                     RGELINCVGVVEKRGWEVESWTEPGDLSELQADFAGWHETVRRLIDNMDPQACFKWAL
                     FDRPPMSAWSDGRVTLLGDACHPTLPFMAQGAAMAIEDAAVLARCLVEGDGVSGSLRR
                     YESLRRARTARVQAGSRRNARLFHSSGPAAGLRNLIVGRASGRVMDWLYGYDALDPGA
                     P"
     gene            3444..3617
                     /locus_tag="AC16QF_08795"
                     /pseudo
     CDS             3444..3617
                     /locus_tag="AC16QF_08795"
                     /inference="COORDINATES: protein motif:HMM:NF046279.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
     gene            3597..4082
                     /locus_tag="AC16QF_08800"
     CDS             3597..4082
                     /locus_tag="AC16QF_08800"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024802.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6448697.1"
                     /translation="MAEYLDEVVPPRLHPEDALKRARNRAWTDFVPTFAAGLSALYYA
                     KSPEGHEERMTVARQRLDKIEEAIRAERGNEGPFFNGPDLSLVDAAYAPFFQRFLIVE
                     QWLATGLLESYPLVGAWADALAASPAVANSVPDVFHDEFVANLKRRGFHVAALMDAAA
                     G"
     gene            complement(4125..5222)
                     /locus_tag="AC16QF_08805"
     CDS             complement(4125..5222)
                     /locus_tag="AC16QF_08805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6448698.1"
                     /translation="MHVGFAPVFQNLGKPVPDHQVYAQELAMARAAEPMGFDSVWSVE
                     HHFTDYTMVPDVTQFLSYMAACTERVQLGSMVIVLPWHDPVRVAEEIAMLDALSGGRM
                     ILGIGRGLGRVEFDGFRVPMDESRERFVESATMILEGLERGFVELDGKHFQQPRRDIR
                     PAPFRTFRGRTFAAAVSPESMPVMARLGVGLLVIPQKPWESVRTDFEVYHRVWAETHG
                     AQSSPPDPMCGGFFFVDESADRAEEMAYKYIGRYYHSVMDHYELRIGHLEGTKGYEFY
                     EKIHEHIETRSDDGAARDFVRLMPFGTPEQVIEKVEFIHRMIGAKGVMTHFAYGGMDY
                     AEANRNMRLFADRVMPELQRLGEQRGALAVA"
     gene            5309..6877
                     /locus_tag="AC16QF_08810"
     CDS             5309..6877
                     /locus_tag="AC16QF_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6448699.1"
                     /translation="MAKGERRQASMYPGSWAQIKGEDVAVLAAGSGDALTWRDLDRRS
                     NQAAHALRGAGLGAGDHLALIMENHLQFFEIAWAAIRSGLYITCVNRYLTAEEAAYIV
                     TDSSAKAVIVSAEIGLGSKLLPLIAECPVRLMARGVEPGWTSYEKTIEACPFTPVADE
                     TVGDSMLYSSGTTGRPKGIKRPLTGQSPAKGIPGVEQMSPYGFSQESVYLSPAPLYHA
                     APFAFCMRTLSLGGTVIMMERFDPLEALGCIERYRVTHSQWVPTMFVRLLNLDDEART
                     GFDLSSHRCAVHAAAPCPVPIKQQMMTWWGPIIWEYYAGTERNGSTLIDPHDWLAHPG
                     SVGRARSGVIHICDDDGRELDPGQEGLIYFEQPERPFEYHNAPDKTANATHPDHGNWT
                     ALGDVGYLDDEGYLYLTDRKAFVIISGGVNVYPREIEDVLIVHPKVADVAVFGVPNEE
                     FGEEVKAVVQPAPGTSPGDALAAELVGHARAHLAGFKVPRSIDFIDEMPRLPTGKLYK
                     QPLRARYWPERKST"
     gene            6874..>7091
                     /locus_tag="AC16QF_08815"
     CDS             6874..>7091
                     /locus_tag="AC16QF_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448700.1"
                     /translation="MTGNSSILSDLPATAGVARSTRREGVDVSLNAPTPAQPAIASPE
                     WAVAGWIIRFVRLMAGHRLAIPQDPGDV"
BASE COUNT         1303 a         2450 c         2217 g         1121 t
ORIGIN      
        1 ccgacctacg ctggggagcc acaagtcccg cgtcatgccg atgccctcac cggacagctc
       61 gatacggccc tcgtcgcccc cggctaccca gctcagaagc gatccgaaat ccgccagcgt
      121 ccgccagacg gaaccaggca cggcgtccat ggcgtggcgg tatccgaccc gacaggattc
      181 acccggcatc gagcgtcacc ttggctcgcg cccgggattg gcgaacggcc ccgatatcat
      241 tctcgtccga atcgtggcgc ggcgactgcg gctgcatccc ttgcgtctcc catttcccag
      301 tgcgcccatt caaacacgat cgacccggcc cgggtgtaaa aggcttgcac gtcatgggtt
      361 aagatttacc aagaggacat tcgccgcgat gtccctgccg gacggccgcg ttcacggcat
      421 ccgggcagcg ctgcgaggca acagcagggg gaagaacgga aatgaacaca aagccactcg
      481 acgccatcgt ggtgggggcc ggcttcgccg ggctctacat gctccaccgc ctgcgcggcc
      541 tgggcctgaa aacccgcgtc ttcgaggccg gcagcgacat cggcggaacc tggttctgga
      601 accgataccc gggcgcgcgc tgcgacatcg agagcatgga gtactcctac cagttctccg
      661 acgagcttgc ccgggaatgg gagtggtcgg agcgttacgc tacccaaccg gaaatcctcg
      721 gctacataaa ccacgttgcc gaacgcttcg atctgcgcgg ggacatacag ctcaacaccc
      781 gggtggagcg agcccggttc agcgaagaaa caaaccggtg gaccgtcacg acgagcgatg
      841 gcggcgagca cgaagcaaag tacctggtaa tggcaaccgg ctgcctttcc tccacgaacc
      901 tgcccgaatt cgatggcatc gacagctttc aaggcgactg gtaccacacc gggcaatggc
      961 cccacgaagg cgtggatttc accggcaagc gtgtcgccgt catcggtacc ggctcgtcgg
     1021 ccatccagag catcccgctc atcgccgagc aggcggagca gctcaccgtc ttccagcgct
     1081 cccccaactt ctccgtgccc gcgcacaatc gggccctgga tccggaagcg gtcgcccgga
     1141 tcaaggcccg ataccccgag ttccgggatg cgaatcggca gacggccttt cacgcggatt
     1201 tccgctacaa cgagtccaac gccctggacg tatccgccga gcagcgcgag cgcgagtatg
     1261 aggcgcgctg ggaacggggc gggttgccct tcctggccgc cttcgccgac ctcgtgttcg
     1321 acgccgacgc caacgagacc gccgccgagt tcatcagagg caagatacgc gagaccgtca
     1381 aagacccgga caccgccgag cttctctgcc cgaccagcgt cgtggggtgc aaacgcctgt
     1441 gcgtggatac ggactactac gccaccttca accgcgaaaa cgtcaccctg gtgagcgtgc
     1501 gggacacgcc catcgaggcc atcacgccca aaggactgcg aacggctgac gaggagtacg
     1561 agttcgacgc catcgtgttc gccaccggct tcgatgccat gacgggggcg ttgatgaaga
     1621 tggacattcg cgggatcggc ggccgttcaa tgcaggacgc atggtcggaa ggccccaaga
     1681 cctacctggg cctggccgtg tccggttttc cgaacctttt caccgtcacc ggccccggaa
     1741 gcccctccgt gctgacgaac atgctcccga cgatagaaca ccacgtcgac tggatcgccg
     1801 actgcgtcgc ctacatggag cacgaaggca tgtcccgcat cgacgcgatg cccgaggccc
     1861 agacagcgtg ggttgctcac gtgaacgagg tgggggattc cagcctgtat ccacgctgca
     1921 actcctggta tctgggtgcg aacattccgg gcaagcctcg cgtgttcatg ccctatctgg
     1981 gttttccgcc ctacgccgaa aaatgccggg aggtggcgga atccggctac gacggttttg
     2041 cgctgagcgc gtgacggaac ccggcggcgc ccatcatggc gcgccaggat ccagcgcgtc
     2101 gtacccgtaa agccagtcca tgacgcgacc cgaagcgcgg cccacgatca ggttgcggag
     2161 cccggcagcc ggcccgctcg agtggaagag ccgcgcgttc cgcctggaac cggcctgcac
     2221 ccgggccgtt cgcgccctgc gcaacgactc gtagcggcgc aggctcccgg aaaccccgtc
     2281 gccttccacc aggcaccgcg ccagcaccgc cgcatcctct atggccatgg ccgcgccctg
     2341 ggccatgaac ggcagcgtcg ggtgacaggc atcacccagg agcgtcaccc gtccatcgct
     2401 ccacgccgac atcggcggac gatcgaacag cgcccacttg aagcaggcct gcgggtccat
     2461 gttgtcgatc agccgccgaa ccgtctcgtg ccatccggcg aagtcagctt gcagctcgct
     2521 gaggtctcca ggctccgtcc aggactcgac ttcccacccg cgcttctcga cgacgcccac
     2581 acagttgatc agctcgcctc tccggacgta gtagtgcacg aaatgcttgt gcggccccca
     2641 ccaggcgctg gcggccggcc ggacgaagcc tgaggggacc gcgcctgccg gcaccaggcc
     2701 ccgccaggcg acgttgcccg tgaaccgcgg cgcttcggcg ccgaacaacg cctcgcggac
     2761 cgtggagtga ataccgtccg caccgatcag caggtcaccg ccatgacgct cgccgccggc
     2821 ctcgacgaag acggcggagt cgcccggctc gataccctcg accctcgcac cggtgcgcag
     2881 ctcgataccc ggtgcccgcc gcagcgcgcg ttccaggacg tcgagcagat cggcccggtg
     2941 cacgtgatag tagggataga cgaaccggtc gcggaaagcc tcgtctatgg ggctagaggc
     3001 cagcaggcgg ccgcttcgcc agtcgcgcat ctccacggcg cgcgggagga aggcgacgct
     3061 gccgagagcg ccctcgagac ccagatgatg aagcacgcga gaggcgttgg gcgaaagctg
     3121 aattcccgcg cccacctcgg cgagatcggc cgtctgctcg aacacgctga cggcaatccc
     3181 ttgccttgcc aggcagagcg cggccgtgag tccgccgatc ccgccaccga cgatgagcgc
     3241 gcgcgatacc tgcaacggta atccatagca tggcttaggc accgatggta taccggcaac
     3301 ttcgctcaga ccaacgcccg agtggtcggc tgcaacctcg cgtacgcttg tcaggtcacc
     3361 gcctgacggc gtagtcttca gccatcatga acgcacccgc gagatatcac ctcatcagct
     3421 ccttcacgtg accctgggtc cagcgcgccg tgatcgtgat gcgcgccaag gacgtcgact
     3481 tcgacgtgac ctacatcgac ctgcgcgaca agcccgactg gtttctggag atttcccccc
     3541 acggcaaggt gcccgtgctg caggtagacg gggtcccgct gttcgagtcc aacgccatcg
     3601 ccgagtatct cgacgaggtg gtccccccgc gcctgcatcc cgaggatgcg ctaaagcgcg
     3661 cccgcaaccg tgcctggacc gatttcgtgc ccaccttcgc cgccggcctg agcgccctgt
     3721 actacgcaaa gtctccggag ggccacgaag aacgcatgac ggttgcccga cagcgcctcg
     3781 acaagatcga ggaggccatt cgcgccgagc ggggcaacga agggcccttc ttcaacgggc
     3841 ccgatctctc cctggtggat gccgcctacg cgccgttctt ccagcgtttt ctcattgtcg
     3901 agcaatggct ggccaccggc ctgctcgagt cttacccgct ggtgggcgcc tgggcagacg
     3961 cccttgcggc cagcccggcg gtcgcaaact ccgtgccgga cgtcttccac gacgagttcg
     4021 tcgccaacct gaagcgtcgc ggatttcatg tagccgccct gatggacgca gcggcgggct
     4081 gagcagggaa gcggccgcag cccgcgggtc gcctgaatcc ggcctcacgc caccgccaag
     4141 gcccctcgct gctcgcccag ccgctgcagc tccggcatga ccctgtcggc aaacagacgc
     4201 atgttgcggt tggcctcggc gtagtccatc ccgccatagg cgaagtgggt catcacgcct
     4261 ttggccccga tcatccggtg gatgaactcg accttctcga tcacctgctc gggcgtgccg
     4321 aacggcatca gccggacgaa gtcccgggcc gcgccgtcgt cggatcgggt ttcgatgtgc
     4381 tcatgaatct tctcgtagaa ctcgtaacct ttcgtgcctt ccaggtgccc gatgcgaagc
     4441 tcgtaatggt ccatgacgga gtggtaataa cggccgatat acttgtaggc catctcctcg
     4501 gcccgatcgg cgctctcgtc gacgaagaag aagccgccgc acatagggtc cggcggagac
     4561 gactgcgcgc cgtgagtttc tgcccagacc cggtggtaaa cctcgaaatc cgttctgacc
     4621 gattcccatg gtttctgcgg gatcacaaga agcccaacac caagccgggc catcaccggc
     4681 atggactccg gggacaccgc cgcggcgaac gtccgccccc ggaaggtcct gaacggcgcc
     4741 ggccggatgt cgcggcgagg ctgctggaag tgcttgccgt cgagctccac gaagccgcgc
     4801 tccagccctt cgaggatcat ggtcgccgat tcgacgaaac gctcacggga ctcgtccatg
     4861 ggcacgcgaa agccatcgaa ctcgacgcgg ccgagccccc ggccgatacc caggatcatg
     4921 cgcccgccgg agagggcatc gagcatggcg atttcctcgg cgacgcgaac cgggtcgtgc
     4981 cagggcagca cgatcaccat ggagccgagc tgcacgcgct ccgtgcaggc cgccatgtag
     5041 ctcagaaact gcgtgacgtc gggcaccatc gtgtagtcgg tgaagtggtg ctccaccgac
     5101 caaacggagt cgaagcccat gggttccgcg gcgcgggcca tggccagctc ttgcgcgtac
     5161 acctgatggt cgggtaccgg cttgccgagg ttctgaaata cgggtgcgaa tccgacgtgc
     5221 atgactttcc tccctctgct taccgcaatt taacatcagc cggaaaatcc atgcgatccc
     5281 tttccctgcc gcccgcggga caggcatcat ggcgaagggc gagcggaggc aggccagcat
     5341 gtatccgggc agctgggcgc agatcaaggg cgaagacgtg gccgtgctgg ccgccggcag
     5401 cggggatgcg ctcacgtggc gcgatctgga ccggaggtcg aaccaggccg ctcatgccct
     5461 gcgcggggcc gggctaggcg ccggcgatca cctcgcgctg atcatggaga accatctgca
     5521 gttcttcgag atcgcctggg cagcaatccg ctcgggtctg tacatcacct gcgtcaaccg
     5581 ctacctgaca gccgaggagg cagcctacat cgtcacggac tcgtcggcga aagccgtcat
     5641 cgtctccgcg gagatcggtc tgggcagcaa gcttctgccg ctgatcgccg agtgccccgt
     5701 gcggctgatg gccagaggcg tggagccggg ctggacgtcg tacgagaaaa ccatcgaggc
     5761 ctgccccttc accccggttg ccgacgagac cgtgggcgat tccatgctct acagctcggg
     5821 caccacgggc cggcccaagg gcatcaagcg gcccctgacc ggccaatcgc cggcgaaagg
     5881 catacccggg gtggagcaga tgagccccta cgggttcagc caggagagcg tctacctgtc
     5941 acctgcgccc ctctaccacg ccgcgccgtt cgcattctgc atgcggacgc tgagccttgg
     6001 cggcacggtg atcatgatgg agcgattcga tccgctggag gccctcggct gcatcgagcg
     6061 ataccgggtt acccacagcc agtgggtgcc gaccatgttc gttcgtttgc tgaacctcga
     6121 cgacgaggcg cgaacgggat tcgacctcag cagccaccgt tgtgccgtcc acgccgcggc
     6181 accctgcccg gtccccatca agcagcagat gatgacgtgg tggggaccga tcatctggga
     6241 atactacgcg ggcacggagc gcaacggctc gaccctgatc gacccccacg actggctggc
     6301 ccatcccggc tcggtaggac gggcgcgcag cggcgtcatc cacatctgtg acgacgacgg
     6361 ccgggagctc gacccgggcc aggaaggcct gatctacttc gagcagcccg agcggccgtt
     6421 cgagtaccac aacgcgcccg acaagacggc caacgccacc caccccgacc acggcaactg
     6481 gacggccctc ggcgacgtcg gctatctgga cgacgagggt tacctctacc tgaccgaccg
     6541 caaggcgttt gtgatcatct ccggaggggt gaacgtctac ccgcgcgaga tcgaggacgt
     6601 gctcatcgtt cacccgaagg tggcggacgt ggccgtgttc ggggtgccga acgaggagtt
     6661 cggcgaggag gtcaaagccg tggtgcagcc ggcgcccggc acgagccccg gcgacgccct
     6721 ggcggcggag ctcgtcggcc atgcgcgcgc ccacctcgcc ggtttcaagg tgccccgctc
     6781 catcgacttc atcgacgaga tgccgaggct gcccactggt aagctctaca agcagccgct
     6841 gcgggcacgt tactggccag aaaggaaatc gacatgaccg gaaactcgag cattttatcc
     6901 gacctgcctg ccaccgcggg agtcgcccgc agcacccgca gagaaggggt ggacgtcagc
     6961 ctgaatgcgc ccacgcccgc ccagccggcc atcgcatcgc cggaatgggc ggtggcgggc
     7021 tggatcatca ggttcgtccg cctgatggcc ggccaccgcc tggccatccc acaggacccg
     7081 ggtgacgttt t
//
LOCUS       DBNHLM010000193         3076 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_87502, whole genome shotgun sequence.
ACCESSION   DBNHLM010000193 DBNHLM010000000
VERSION     DBNHLM010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_87502"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="AC16QF_08820"
     CDS             complement(<1..277)
                     /locus_tag="AC16QF_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448701.1"
                     /translation="MRRRLAVIAAALALIVGAHGAGAESAHRIIAISYHDVVEHPSEV
                     GADRFTVTVDQLINQLAWLDAHGFEPITLDQWAAAAEGEPLPPRPVLL"
     gene            complement(274..2724)
                     /gene="pgaA"
                     /locus_tag="AC16QF_08825"
     CDS             complement(274..2724)
                     /gene="pgaA"
                     /locus_tag="AC16QF_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HIR6448702.1"
                     /translation="MSIWPARRGGRRAGRWAALLLALVAPLVTASVDRSREEAVALAR
                     AGDVAGAVETLERLHADAPESLPLRLDLAVISSWAGNDSRTLELLIDLEPGDLPTYVL
                     ETYARSARNQASWELSFDLYDRLIEREPHVLGHRLGLALAMADGGEAKAARRTLEEAR
                     AVAGDEPKDLAQVELVCGYVEERDGRFTRALVCYDDALALDPGLGEARRRWIMLASAL
                     GATVEAFEAAQANPEVLTPDDVVRLQLDAAAKRIRWADLPSPGAPLADAEMAVDGHDV
                     VAKGGEPARVAGSHRRALEYDRVVSHSAAYRMVEAIDLFESLQGTPGDLEGFPSYVLA
                     AAGRAYLYEEHTRDAIACFEQALGLDPNAFNLRIGLFYALSDHGDFEGAKEVAEHLIA
                     GEEPFARPDPRVWVPNDRYATARVIAARELAYRERYTEALNDLDEMLAIAPANTGVRL
                     TRAQLLQWRGWNEQAEREVVRIQTTDPTNVGVKVLSGTVALEKQQYPRAEREVNRALR
                     SQPYDKSAIELNERWLLHNSGYFEASASAERSEGSAFAGESWRTDVHYYSSPIAYRYR
                     WFVHDAIRHGKFTEGTGKDHRIGAGVDFRSNRWSARGEVHKGLEQNRKAGVTASASWR
                     QSDRLTWDSLVSYNTMGMPLRGTRVGIRADEAQGGATYRWHESRRAYARAGLMKMDDG
                     NDRKWLDSGFEQRVVNAPRQKLLVNLGAYTSRNSGGDRVYYNPETDYQITAGLTHEWR
                     LMRRYQQSFVQRLSVDGGEYHQKGFGGGPIWTLRLEHDWQIGRRVGMSWGATWGGRRY
                     DGEREHVTTVFLTFRGHL"
BASE COUNT          509 a         1008 c         1019 g          540 t
ORIGIN      
        1 tgagaagcac cggccggggc gggagcggct ctccctcggc tgcagccgcc cactggtcga
       61 gggtgatggg ctcgaagccg tgggcgtcga gccaggccag ctgattgatg agctgatcta
      121 cggtcactgt gaagcggtcg gccccgacct cagagggatg ctccaccacg tcgtggtagg
      181 agatcgcgat gatccgatgg gccgattcgg caccggcacc atgcgcgccg acgatgagcg
      241 ccagcgcggc ggcgatgact gccagccgcc gcctcacaga tgtccccgga aagtcaggaa
      301 cacggtggtc acgtgctcgc gctcgccgtc gtagcggcgc ccgccccagg tggctcccca
      361 gctcatgccc accctgcgcc cgatctgcca gtcgtgttcg agccgcaagg tccatatcgg
      421 gccgccaccg aaacccttct ggtggtactc accgccgtcc acactcagcc gctgaacgaa
      481 ggactgctgg tagcgccgca tcagacgcca ctcgtgggtc aggcccgccg tgatctggta
      541 gtcggtctcc gggttgtagt agacccggtc gccgccggag ttccggctcg tgtacgctcc
      601 caggttgacg agaagcttct gccgaggggc gttgaccacg cgctgctcga agccggaatc
      661 cagccacttg cggtcgttgc cgtcgtccat cttcatcagg cccgcacgcg cgtacgcgcg
      721 tcgggattcg tgccagcgat aggtcgcgcc accctgagcc tcgtccgcgc ggatgccgac
      781 gcgggtgccg cgcaacggca tccccatggt gttgtaggag accaggctgt cccaggtgag
      841 acgatcggac tgccgccagc tcgccgatgc cgtcacgccc gccttacggt tctgctccag
      901 gcccttgtgc acctcgccgc gcgcgctcca gcgattgctg cggaaatcca cgcccgcgcc
      961 gatgcggtga tccttgccgg ttccttccgt gaacttgccg tggcggatgg cgtcgtgaac
     1021 gaaccaccgg tagcggtagg cgatggggct cgagtagtag tgcacgtccg tgcgccagct
     1081 ctcgccggca aaggccgacc cctcggatcg ctccgcgctg gcgctggcct cgaaataccc
     1141 gctgttgtgc agaagccatc gctcgttgag ctcgatggcg gacttgtcat agggctgtga
     1201 tcgaagagcg cgattaactt cccgctcggc tcgcgggtac tgctgttttt cgagggcaac
     1261 cgtgccgctc agcactttga cgccaacgtt ggtcgggtcg gtcgtctgga tccgcaccac
     1321 ttcgcgctcg gcctgctcgt tccagccacg ccactgcagc aattgcgctc gcgtgagccg
     1381 cacgcccgtg ttggccggcg cgatggcgag catctcgtcc agatcgttga gcgcctcggt
     1441 gtagcgttcc cgataggcga gctcccgagc cgcaatgacc cgcgccgtgg cgtagcggtc
     1501 gttgggcacc cagactcgcg gatccggtcg ggcgaacggt tcttcgccgg caatcaggtg
     1561 ctcggcaacc tctttcgcgc cttcgaagtc accgtgatcg gacagcgcgt agaaaagccc
     1621 gatgcgaagg ttgaaagcgt tcgggtcgag cccgagcgcc tgctcgaagc aggcaatggc
     1681 atcgcgggta tgctcttcgt acaggtaagc gcggccggcg gcggcgagaa cgtaggaagg
     1741 aaatccttcc agatcgccgg gcgtaccctg caacgactcg aagaggtcga tggcctccac
     1801 catccggtac gctgcggaat gggagacgac gcggtcgtac tccagcgcgc gtcggtggct
     1861 acctgcgacc cgtgccggtt ctccgccttt ggcaaccaca tcatggccgt ccacggccat
     1921 ttcggcgtcc gccagcgggg cacccgggct cggcagatcg gcccagcgga ttcgtttcgc
     1981 cgccgcatcg agctgcaggc gcaccacgtc gtcgggcgtg agcacctcgg gattggcctg
     2041 agcggcttca aacgcctcga cggttgcccc gagagcggat gccagcatga tccagcgtcg
     2101 gcgcgcttcc cccaacccgg ggtcgagggc gagggcatcg tcgtagcaga ccagcgctcg
     2161 ggtgaatcgg ccatcacgct cttccacgta gccgcaaacc agctcgacct gagccaggtc
     2221 tttcggctcg tcgccggcaa ctgcacgggc ctcctccagc gtcctgcggg cggcctttgc
     2281 ctcgccgcca tccgccatcg cgagagccag gccaagccga tgaccgagga cgtgaggctc
     2341 gcgctcgatg agccggtcgt agagatcgaa ggaaagttcc caggacgcct gattgcgcgc
     2401 cgaacgggcg tacgtttcca gaacgtaggt gggcagatca ccgggctcga gatcgatgag
     2461 gagttcgagg gtccgcgaat cgtttcctgc ccaggaagaa atgacggcga ggtccagccg
     2521 aagcggaagc gactcggggg cgtcggcatg cagtcgttca agggtctcta ccgcgccggc
     2581 aacatcccct gcacgagcga gcgcaaccgc ctcctcacgg gagcggtcca cactggcggt
     2641 gacaaggggt gcgaccaatg cgagaagaag ggcagcccat cttcctgcac gtcggccccc
     2701 acgtctggcg ggccaaatag acaccagcaa aagcggttcc tgcagcgatc ctttgcgtag
     2761 atcccattct cccgatatcg ctgttcgcga tgtgatcggg cgtcccgatc tgtgacgggt
     2821 tccgcaccag ctgtgcgagg tggtccccta agaggtcttc ggcgcgagtg aggttcgctg
     2881 caggtcagag gcgagagtga gagaggggcg gaagccaggc ttccgccccg tggggacgaa
     2941 tcagggtttg atcgcggtaa cggagtcgcc gatgggtgaa cccgagccgt cgtgattgtc
     3001 accgggggtg taatccggcg cgctcagcga gctgatggtg tacgtcacgc tgttgttctt
     3061 cttgtgctgg agcacc
//
LOCUS       DBNHLM010000194         4887 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1404245, whole genome shotgun sequence.
ACCESSION   DBNHLM010000194 DBNHLM010000000
VERSION     DBNHLM010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1404245"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..122
                     /locus_tag="AC16QF_08830"
     CDS             15..122
                     /locus_tag="AC16QF_08830"
                     /inference="COORDINATES: protein motif:HMM:NF044651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448703.1"
                     /translation="MVLGRDWAVIPCDDLLASLRSEFGDDRVAFRYAGN"
     gene            127..1404
                     /gene="tilS"
                     /locus_tag="AC16QF_08835"
     CDS             127..1404
                     /gene="tilS"
                     /locus_tag="AC16QF_08835"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR6448704.1"
                     /translation="MDAGQLVEEALRRALRGAVAPGRVFVGYSGGMDSTVLLHAAIAA
                     SPVPVTAVHANHGLDPQAGRWQAHCERVCAQWGVGLESREVSVGHGNVEAAARAARYG
                     FFESVLSASDLLLLAHHQDDQAETVMLRMVQGRGLIGIPSARRLGAGELRRPFLELPR
                     QVLAEYADGLALDWVEDPGNADEGLDRNYLRSRVLPSLRRRWPGFAGQMQVLLDQRAS
                     TEQMLLARVDGDFESIAVGELLCGDGGSGAELLGIWLSSLGAALPSRGALASFVAQLG
                     SAADRQPELKLAQGSLRRYRGRVYRVADPPALAPSYELIPPGELRLPHGTVTVCEAHG
                     GGFQLHGPLKVVFRGPEGAGHIRCRGHRRSIKKLLQDAGLPPWERLTYPLLEDADGVA
                     AVPGIAERDPDPDPDPPSGGTRWVARWTAHSIR"
     gene            1486..3141
                     /locus_tag="AC16QF_08840"
     CDS             1486..3141
                     /locus_tag="AC16QF_08840"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR6448705.1"
                     /translation="MTRFIFVTGGVVSSLGKGIASSSLAALLEARQLNVNVLKMDPYI
                     NVDPGTMSPFQHGEVFVTADGAETDLDLGNYERFTHARMSRRNNFTTGSVYAEVLRRE
                     RRGDYLGGTVQVIPHITDEIKRRINEAGQGFDVLIVECGGTVGDIESQPFLEAIRQMR
                     LEVGAANAIIIHLVLVPFVPSAGEIKTKPTQHSVKELRSIGLQPDLLVCRSDRPLPAS
                     AAGKIALFTNVEERAVISLPDAESIYQVPQALRDGGIDDIILDKMGLDCPPADLSEWG
                     RVVDAQLHPERAVRIAIVGKYLDLLDAYASLIEAITHAGIQTRIRVDIDFIDAEDLER
                     SGTGALERVNAILVPGGFGQRGFEGKIAAARYARENGLPYLGICYGLHAAVIDYARHV
                     CGLDGAHSTEIDAETPHPVIALITEWLDRDGQIEQRTSETDLGGTMRLGEQVCVLKDG
                     TIAQRMYGAETVRERHRHRYEVNNQYIERLEAAGLVVAGRSEDGELVEMIELSGHPWF
                     VACQFHPEFTSTPRAGHPLFSGFIGAASDRAAAAPVLRVGTSD"
     gene            3157..4002
                     /gene="kdsA"
                     /locus_tag="AC16QF_08845"
     CDS             3157..4002
                     /gene="kdsA"
                     /locus_tag="AC16QF_08845"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HIR6448706.1"
                     /translation="MRLCDFEVGLDRPLFLIAGPCVIESEQLALETAEALKALTDELA
                     VAFVYKSSFDKANRSSHESFRGPGLEAGLRILERVNREVGVPVLTDVHEDTPLDEVAA
                     VVSVLQTPAFLCRQTDFIQRVCSQGLPVNIKKGQFLAPADMIHVVTKARATGNEQILV
                     CERGASFGYNNLVSDMRGLAIMRATGCPVVFDATHSVQMPGGLGAASGGQREMVPVLA
                     RAAVAAGISGIFMETHPDPDQALSDGPNSWPLSLMAPLLRQLVDLDRVAKERPFLEDQ
                     YAQGR"
     gene            4006..>4887
                     /gene="eno"
                     /locus_tag="AC16QF_08850"
     CDS             4006..>4887
                     /gene="eno"
                     /locus_tag="AC16QF_08850"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR6448707.1"
                     /translation="MSRIAAVIGREILDSRGNPTVQADVVTDDGFMGSACAPSGASTG
                     SREALELRDGDASRYLGRGVLNAVGAVNGEVAKALAGVDPADQEAIDALLCELDGTPN
                     KGRLGANGLLAVSLAAAKAAAAAKNQALFEHINDLFGGVAMRMPLPMMNILNGGAHAD
                     NNVDIQEFMVQPVSMGSFREGLRCGAEVFHALGGLLRSRGLTTSVGDEGGFAPDLASN
                     AHALAVVAEAVSDAGYELGKDVTLALDCAATEFYADGQYRLAGEHRDLSSEEFAAYLE
                     DLTARYPVVSIEDGMDEN"
BASE COUNT          814 a         1547 c         1673 g          853 t
ORIGIN      
        1 gggcccgggg ccgcatcgtg cttggtcgcg actgggccgt catcccctgc gatgatcttc
       61 ttgcgagcct ccgctcggaa tttggcgacg atcgcgtggc tttcagatac gccgggaact
      121 gatcccgtgg acgccgggca gctggtcgaa gaggcgctgc gaagggcatt gcgcggtgcc
      181 gtcgcaccgg gccgagtctt cgtcggctac agcggcggca tggactccac ggttctgctg
      241 cacgcggcga tcgccgcgtc tccagtgccc gtcaccgctg tgcatgccaa tcacggcctg
      301 gatccccagg ccgggcgatg gcaggcacat tgcgagcggg tctgcgcgca gtggggggtg
      361 ggtcttgaat cccgcgaagt gtcggtaggc cacggcaacg tggaagccgc tgcccgggcc
      421 gcccgttacg gcttcttcga atcggttctc tccgcatccg acttgctgct gctggctcat
      481 caccaggacg accaggcgga gaccgtgatg ctgcgaatgg ttcagggccg ggggctgatt
      541 ggtatcccgt ctgcccgtcg gctgggcgct ggcgagctgc ggcgaccatt tctggaactg
      601 ccgcggcagg tgctggcgga gtatgccgac gggctcgccc tcgattgggt agaggatccc
      661 ggcaatgccg acgaaggtct ggaccggaac tatctgcgca gtcgcgtcct gccgagcctg
      721 cgccggcgct ggccggggtt tgccgggcag atgcaggttc tgctggatca gcgggcgagc
      781 accgagcaga tgctgctggc aagggtggac ggcgacttcg agtccattgc cgtcggcgag
      841 ctcctttgcg gggacggcgg ctccggggcg gagctgttgg gcatctggct gagctctctc
      901 ggggccgcgc tcccctcccg aggcgccctc gcctcgttcg tggcgcagct cggaagcgcc
      961 gccgaccggc aaccggagct caagctggcg caggggtctc tgcgccgcta ccggggccgc
     1021 gtctatcggg tcgcggatcc gcccgctctg gcgccgagct acgagctgat cccgccgggg
     1081 gagctgcggc tgccccacgg caccgtcacc gtgtgcgagg cgcacggcgg gggctttcag
     1141 ttacacggcc cgctcaaggt ggtgttccgc ggccctgaag gagcaggaca catccgctgc
     1201 cgcggccatc gccgctccat caagaaactg ctccaggatg cgggtcttcc gccctgggaa
     1261 aggctcacct atccgctgct cgaggacgca gacggggtag ccgcggttcc gggcattgcg
     1321 gagcgggacc cggacccgga cccggacccg ccgtccgggg ggacgcgctg ggtcgcccga
     1381 tggacggcgc attcgattcg ttgaggttgc cgggggcttt tgttatcttt caaggccatc
     1441 ttgcgttcgc agcaatcgcg gacctcgggc ttcaagatgg aatagatgac acgattcatt
     1501 ttcgttactg gcggtgtcgt atcctctctg ggtaaaggta tcgcctcgtc gtccctcgca
     1561 gcgctcctcg aggcaaggca actcaacgta aacgtgttga agatggatcc ctacatcaat
     1621 gtggatcccg ggacgatgag cccgtttcag cacggtgaag tcttcgtgac cgccgacggg
     1681 gcggaaacgg atctggatct cggcaactac gagcgtttca cccacgcccg catgtcgcgg
     1741 cggaacaact tcaccactgg cagcgtctac gccgaggtgc tgcggcggga gcgccgtgga
     1801 gactacctgg gcggaacggt tcaggtgatc ccgcacatca ccgacgagat caaacgcagg
     1861 atcaacgaag ccgggcaggg cttcgatgtg ctgatcgtag agtgtggggg gacggttggt
     1921 gacatcgagt cgcagccgtt cctggaggcc attcgccaga tgcggctgga agtgggcgcg
     1981 gcgaacgcca tcatcattca tctggtgctc gtccccttcg tgccgtccgc cggggagatc
     2041 aagaccaagc ccacccagca ctcggtgaag gagctcagat ccatcggcct gcaacccgat
     2101 ctcctggtct gccgttccga ccgacccctg ccggcgtctg ccgccggcaa gattgcccta
     2161 ttcacgaacg tggaggaacg cgcggtcatc tcgctgccgg acgccgagtc gatctatcag
     2221 gtgccccagg ccctgcgcga cgggggtatc gacgacatca tcctcgacaa gatgggcttg
     2281 gactgcccgc ccgccgacct cagcgagtgg gggcgtgtgg tggatgccca gctccatccc
     2341 gagcgggctg tgaggatagc catcgtcggc aaatacctgg acctgctgga cgcgtacgcc
     2401 tcgctcatcg aggccatcac tcacgccggt attcagacga ggatccgggt ggacatcgac
     2461 ttcatcgatg ccgaggacct cgagcgttcc ggcaccgggg ccctggaacg cgtgaacgcc
     2521 atcctcgttc cgggcggttt cggccagcgg ggcttcgagg gcaagatcgc agccgcccgc
     2581 tacgcgcgcg agaacggtct gccctatctg ggaatctgct acggtctaca tgccgccgtg
     2641 atcgactacg cccgccacgt ctgcggattg gacggtgctc attccaccga gatagacgcc
     2701 gagacgcctc acccggtcat tgccctgatc accgaatggc tggatcggga cggccagata
     2761 gagcagcgca ccagcgaaac cgacctcggc ggcacgatgc ggcttggcga gcaggtgtgt
     2821 gttctcaagg acggcaccat agcccagcgg atgtacggcg ccgagaccgt ccgagaacgg
     2881 caccgacacc gctacgaagt gaacaaccag tatatcgagc ggctggaagc cgccggactg
     2941 gtggtggcag gacgctcgga agacggcgag ctggtggaaa tgatcgagct gagcggccat
     3001 ccgtggttcg tagcctgcca gttccacccg gaattcacgt cgaccccgcg ggccggacac
     3061 cccctgtttt cgggcttcat cggggcagcg agcgaccgcg ccgccgcggc tccggtgctg
     3121 agagtcggca cgtccgacta gcaaggaacc cccctcatgc gcctctgcga tttcgaggtt
     3181 ggtctggacc ggccgctctt cctcattgcc ggtccctgcg tgatcgagtc tgagcagctg
     3241 gcactggaga ccgcggaggc gttgaaagcg ctcaccgacg agctagccgt tgccttcgtc
     3301 tacaaaagct cctttgacaa ggccaaccgt tcgtctcacg agagttttcg cgggcccggg
     3361 ctcgaggccg ggttgcggat tctggaacgc gtcaaccgcg aagtgggcgt acccgtgctg
     3421 accgacgttc acgaagacac cccgctcgac gaggtggccg ccgtggtttc ggtgctgcag
     3481 acgccggcct ttctctgccg gcaaacagac ttcatacagc gggtctgcag ccaggggctg
     3541 cccgttaaca tcaagaaggg tcagtttctc gcccctgcgg atatgattca cgtggtcacg
     3601 aaggcgcgcg caacgggcaa cgagcagatc ctggtctgcg agcggggcgc gagcttcggc
     3661 tacaacaacc tggtctcgga catgcgcggg ctcgcaatca tgcgagccac gggctgcccc
     3721 gttgtcttcg acgcgaccca ctcggtgcag atgccgggcg gcctgggcgc tgcctcgggg
     3781 gggcagcggg agatggttcc ggtgctggcc agagcggcgg tggcggccgg gatatcgggg
     3841 atattcatgg agacccatcc cgatcccgat caagcgctat ccgacgggcc caattcctgg
     3901 cccctgtcgc tgatggcgcc cctgctccga cagctggtgg acctggaccg cgtggcgaag
     3961 gagaggccgt ttctcgagga ccaatatgca caaggaagat aggacatgag cagaattgcg
     4021 gcggtaattg gccgcgagat tctcgattcg cggggtaacc ccacggtgca ggccgacgtc
     4081 gtcaccgacg acggcttcat gggctccgcc tgcgctccct ccggggcgtc cacagggtcc
     4141 agggaggcgc tcgagctgcg cgacggcgat gcatcccggt atcttggcag gggcgtgctg
     4201 aacgcggttg gggccgtaaa cggggaggtt gccaaggcgt tggcaggcgt cgacccggcc
     4261 gaccaggagg cgatcgacgc actgctttgc gagctcgacg ggacgccaaa caagggcagg
     4321 ctcggcgcaa acgggttgct tgcggtgtcc ctggcggccg cgaaagcggc ggcagcggcc
     4381 aagaaccagg cgctcttcga gcacatcaac gatctcttcg gtggtgtcgc gatgcgcatg
     4441 ccgctgccca tgatgaacat cctcaatggc ggcgcccatg cggacaacaa cgtggacatc
     4501 caggagttca tggtgcagcc ggtctccatg gggtcgttcc gcgaagggct gcgctgcggg
     4561 gcagaggtat ttcacgccct gggggggctg ctgcgctcgc ggggcctcac cacctcggtc
     4621 ggggacgaag gcggcttcgc tccggacctg gcctcgaacg cgcatgctct ggccgtggtc
     4681 gccgaagcag tgagcgacgc cgggtacgag ctcggaaagg acgtaacgct cgcgctggat
     4741 tgcgctgcca cggagttcta cgcggacggt cagtaccgtc tggccggcga gcatcgtgac
     4801 ctgtccagcg aggagttcgc cgcctatctc gaagacctga ctgcgcgcta tccggtggtc
     4861 tccatcgagg acggcatgga cgagaac
//
LOCUS       DBNHLM010000195        22266 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_87524, whole genome shotgun sequence.
ACCESSION   DBNHLM010000195 DBNHLM010000000
VERSION     DBNHLM010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 22266)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 22266)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22266
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_87524"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1620
                     /locus_tag="AC16QF_08855"
     CDS             <1..1620
                     /locus_tag="AC16QF_08855"
                     /inference="COORDINATES: protein motif:HMM:NF025114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6448708.1"
                     /translation="EVTSDYGEFLHAELALDGADPYASEGLDEISEAARREFKVIIVG
                     AGMSGLLAAIRLKEAGISWEIVEKHADVGGTWYQNGYPGCRVDSPNHIYSYSFRPADW
                     PQYYSSQEVLREYFDKVATEYGLRDQIRFSTEVQSMAFDDASGTWQVEVTNGTGARET
                     LTANAVISAVGQLNRPKWPDLAGQDRFRGVSFHSTEWEHEHDLSGKRIIVIGTGASAF
                     QFAPEIAKVAAEVEIFQRTPNWMAPVATYHDHIPLGKHWLLNHVPYYAKWYRFAMFWN
                     MAEGVLSAVRKDPAWNQTEDAISAENDTLRQMFTDWLEELCEGDEELLEKVVPKFPVG
                     GKRVLLDNGNWIRALRRDNVHLLTDPIAEINETGVVTEDGKQHDADVLIYGTGFQASR
                     FLWPMKVTGRDGIDLNEQWQGDARAYLGITIPNFPNLFCCYGPNTNIVVNGSIVFFSE
                     CEVRYILSCIRQLLAGGHKALDCRADVHDAYNEWIDEGNANMAWGAARVPSWYKNEKG
                     RVSQNWPFTLLEYWNHTRDVNAADYEFLSDA"
     gene            1701..2330
                     /locus_tag="AC16QF_08860"
     CDS             1701..2330
                     /locus_tag="AC16QF_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HIR6448709.1"
                     /translation="MILDLHTHSIKSDDGRAKVQNYCQWIRTREIPIDGLVLTEHRQF
                     DAESDYSPLAQQFGITILKGSEVETEYGHVLVFGVTEALTRAFDFADIHLPLARVIEA
                     CALHGAVPVPCHPGRRRVGMSAHLEEFGTPEGVRIVETHNGGSRGDEDAVAQAMAAEL
                     GYLGIGGSDAHIVSHVGRCATRFPGTISNEQELVEALLAEEFEAVTNRE"
     gene            2340..2783
                     /locus_tag="AC16QF_08865"
     CDS             2340..2783
                     /locus_tag="AC16QF_08865"
                     /inference="COORDINATES: protein motif:HMM:NF024844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAS1-like dehydratase domain-containing protein"
                     /protein_id="HIR6448710.1"
                     /translation="MLDLEALRANFLNEDFDEQHFEVNAEQCVAYALACGETAPRYTD
                     PAHPDFQAPPTLPSSFHPGRRLPDGFPKLPGLGMDAGKAVAPKRPIRPGVPLVGRTHL
                     HDVYAKTGRSGRMTFMVTRMEIYDPDGEQLATADTRIVIRERPDQ"
     gene            2780..3193
                     /locus_tag="AC16QF_08870"
     CDS             2780..3193
                     /locus_tag="AC16QF_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6448711.1"
                     /translation="MTVNVIGDVEFGEELPVFEPDTSLANVKRFTEAAGWQGPRFTDH
                     EAARREGLPGALVPGIMSQGFLAAMIHRWAPESQIESVDTVFRAPVLVDERHSISGVV
                     TDIDEDAGTVEIDLTVTNEANETRVFGTATVRLPA"
     gene            complement(3247..3651)
                     /locus_tag="AC16QF_08875"
     CDS             complement(3247..3651)
                     /locus_tag="AC16QF_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6448712.1"
                     /translation="MSEAKRPLPNLHEMDSRAFWEATREHELRYQECADCGTVVFYPR
                     RHCTGCLGDALAWKTAKGEGTVYTFSVIRQSYHPFFRALVPYAVAWIDLDEGPRLLSN
                     VIGPVEDIRVGQRVRVAWEDHEELAVPLFEPI"
     gene            complement(3663..4859)
                     /locus_tag="AC16QF_08880"
     CDS             complement(3663..4859)
                     /locus_tag="AC16QF_08880"
                     /inference="COORDINATES: protein motif:HMM:NF047238.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR6448713.1"
                     /translation="MAGQARSAAIVGIHEYPSRDVEGSVSPLQIKAESAARALADAGL
                     SWQDVDAIYDAGEAGMMSGLTIAEYLGLKPNVIDTTNVGGSSYEYHAAHAARDIAAGK
                     ARVALLTYGSTAHSNARAIGVGGRGGGGMHPADNMDAFAGMTLVANYAMVAQRHMYEY
                     GTTSEQLAEISVATRCHAMRNPEAVKAMEDLEFLDIRETTVEDVVGSRMIADPLHLLE
                     CCMISDGGGAVVIASPEVARNCARPPVWILGTGEATKYPENGGDITVSAAAQSGPQAF
                     AEAGVSPAEMDIAMIYDSFSITVLTILEDLGFCAKGEGGPWAAGGRLRFDRPADGPAL
                     NTDGGGLSSNHPGMRGIFLLVEAARQLRGDSCSQVDGAKLAVAHGNGGMLGSRHCAGT
                     VILGRD"
     gene            complement(4932..6251)
                     /locus_tag="AC16QF_08885"
     CDS             complement(4932..6251)
                     /locus_tag="AC16QF_08885"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6448714.1"
                     /translation="MKEQSRYDHLREKAVLAPTTPTRRRQRIPFDPQLDPYEIPLQDL
                     NVANPELFRAEAFWPYFRRLREEAPVHYCSESQYGPYWSITRYDDIMQIEKNHAVFSS
                     EFSLGGVTLMGTPSSGNDVPMFIQMDPPKHDAQRKAVAPKFTQRNLGDLEATVRERAG
                     RILDGLPRNETFNWVEHVSIELTAQMLATLFGVPQEERHQLIRWSNIISNADNPEVVK
                     EKSSYFEALAQCGDYFDALWRDRKSSAPTGDLVSMLAHGPDTADMNSQELLGNAVLLT
                     VGGNDTTRNSISGGLLALNQFPEQYDKLLENADLVRTMVPEIIRWQTPLTHMRRTALA
                     DTEFRGQLIGKGDKVVMWYISGNRDPDAVERPDEFIIDRRRPREHLSFGFGIHRCLGN
                     RLAELQLRVLWEEILKRCPRIEVVGEPERASSVLFRAIQSLPVRIAA"
     gene            complement(6288..7049)
                     /locus_tag="AC16QF_08890"
     CDS             complement(6288..7049)
                     /locus_tag="AC16QF_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448715.1"
                     /translation="MRTLVIQSASELDRPGWLSSCLQSVEGWARERGYDYRFRGDDAL
                     ELTPGWYRAKLIGRSPIVADLARLLLIRDALAKGYERACWIDADVLLYAPERLDLPDD
                     DECAFGREYWVQKNRDGRWQVRRNVHNALCSFVAGCPVLPFLIHATQRIISRADPQHI
                     APQMVGPKLLSALHNLTGFGLIDTIGALSPAVLDDVVQGGGEALRAMLAATPEPLAGV
                     NLCASVNGHRDLAALCRRLTDCSGLHLSGATHPAS"
     gene            7144..8094
                     /locus_tag="AC16QF_08895"
     CDS             7144..8094
                     /locus_tag="AC16QF_08895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR6448716.1"
                     /translation="MDWQAKLNEGGIVLLDGGTGSELQRRGVPMSGAAWSGAAAHTHP
                     DVLRAVHHDYIAAGAEVIITNTFGTSRATLRAAGLGDAFASINRRAVALAMEARELAG
                     RDVAIGGSISNLPPNMNIGDYPDPAEELADLRDQAAILAEGGVDFIALEMMQDVNHAP
                     RAMRAAVATGLPVWLGVSCRRSTQTASLVAFDFADTPFEAPLDALIAMRPAVVNVMHS
                     EVEATVPAIEAVRERWPGPIGAYPEIGYFAAPDRRFGDDHPPDRLVSEARRWVAAGAR
                     LIGGCCGTTPDHIRALHAALPELERLAGETETPGRSPTVR"
     gene            complement(8117..9151)
                     /locus_tag="AC16QF_08900"
     CDS             complement(8117..9151)
                     /locus_tag="AC16QF_08900"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR6448717.1"
                     /translation="MQVGLVTGKETVDLVEMPDPRPEPGKAVVDVSYCGICGTDVHAY
                     QSGEPYNPAICGHEWMGHVSAAGAGVQVKEGDRVAIGMATACGQCATCVRGDPSHCEM
                     AFAGMVGIGPMAAPHGGFASAIAIEAARLYHVGPEISDEAAAILEPVTIAVHAVRRTG
                     IRLGDSVVVVGAGPIGLLVLQCARAAGAGVCVLVEPQATRRETGAALGADHTIDPRET
                     PDVAAAINSIIGKSGADVVFECAGLARTVEEVPAYVRRGGTVALVGYPTGLSQISAAQ
                     WLAQELKVSTALGYVRDDFEVSKGLVADGRVDCGPLHTSTVSLAGMAGAFAELASEPE
                     QIKILVDPRA"
     gene            complement(9221..10012)
                     /locus_tag="AC16QF_08905"
     CDS             complement(9221..10012)
                     /locus_tag="AC16QF_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR6448718.1"
                     /translation="MARLISLASGTLPEFGPAEVTRAAAAGGFNGCGIWFDAATWTDE
                     TTRGVAAAFADTGLTPLEIEVIDIRPGAPDPEHQRLLESGAAVGATEAIVVSSDPDEV
                     AVCRRFAHLCELGEQLGVNVCLEFLPIYAVRDLPSALRIIRDVGHPRGKLLIDPLHVA
                     RSGASVADIAAVPGELLSFAQFCDGAKEPPGEPTLDVLREEAVNGRFTPGAGQLPLRD
                     LLDVLPDDLPLSLELRSRPLRERFPDPAERARHVYRETARFLGGA"
     gene            10292..11062
                     /locus_tag="AC16QF_08910"
     CDS             10292..11062
                     /locus_tag="AC16QF_08910"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR6448719.1"
                     /translation="MRLVSWNVNGLRACAKKGFLGFLETSGADFVALQEVRAFPDQLD
                     DGLRAPPGWHVCFSPAERPGYSGVALYARAAPDRVQTSLGKPDFDREGRLLSAHFGRL
                     AIVSVYFPKGSGKDRDNSRVGYKLDFYAAVFDHVEQLRRRGPVFVVGDYNTAHEPIDL
                     ARPKTNTKSSGFLPEERAEITRWIDAGWVDVFRRQHPGQPDHYTWWRQWGNARRDNVG
                     WRIDYVLASPSAARRVRQAFIWPEVTGSDHCPVGVDFE"
     gene            11276..11581
                     /locus_tag="AC16QF_08915"
     CDS             11276..11581
                     /locus_tag="AC16QF_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448720.1"
                     /translation="MCRHYDESASAGRCREERADPPPEKDVANFCDWFQPGKNAFQEG
                     RTTAGENARSRLDALFGDSQGPAEEPDEAPPKPEDGPRSAEEKARAELDRLFSRDAD"
     gene            11892..13649
                     /locus_tag="AC16QF_08920"
     CDS             11892..13649
                     /locus_tag="AC16QF_08920"
                     /inference="COORDINATES: protein motif:HMM:NF006624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HIR6448721.1"
                     /translation="MSPTTRCSGISHRTRGFMSLVDALEYAAQGDTGLNFYDGSGSLS
                     AALPYAQLAQDARILATRLLGLGVKRGARVGIVAETDPMFPRFFFACQYAGLIPVALP
                     AGLQMGGGEAYVEQLSRMLESCGAAVAVAPESHLSFLKRATRQLSLRMSGSPEDFDAL
                     PAVPVKLLPLSADEPAYLQYTSGSTRFPRGVEMTQTAVLANLTEIAEIGTRITADDRI
                     VSWLPYYHDMGLVGCLLVPVATQISADFLSPRTFAMRPRLWLKLLSENRGTISSSPPF
                     GYELCATRVRMTDTERYDLSAWRVACVGAERINPRPLKQFANVLAPAGFNPKAFLPCY
                     GMAEVGLAISFAPLEDGYFVDDVAKASMSAEGLAIEATEGDRAKGNTLSFVDCGAALP
                     SYDVSIRDESGRELDERECGRIWVRGPSLMRGYFRDPVTTAEVMKPGGWLDTGDIGYR
                     IGRRLFLTSRAKDVIIINGRNIWPQDLEQLAERLPEVRLGAISAFSVTRPNQEELAVM
                     VVESRNAGRELSDELSGMVREHFGINCYIELAPPRTLPRTSSGKLSRARARADFLDRT
                     AWDADGYPMTAPEVQAQAG"
     gene            13667..14599
                     /locus_tag="AC16QF_08925"
     CDS             13667..14599
                     /locus_tag="AC16QF_08925"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6448722.1"
                     /translation="MPDNPTRPLIALTGATGFIGRHLQRALLDAGCAVRALVRTRSLA
                     SSGLIEGVEVAEADLGCESSMKLALNDVDQLVYAAGSVRGRDPEDFALANVTGVKLAA
                     TALASRKPGIPMLLISSLAASAPELSHYAASKRAGEDALRGSGLTRWAIMRPPAVYGP
                     GDRELKGTFTAIRRGLVPMVAPADQHLPFIEVGDLADAVNAWSQHPQACSGGTFALHD
                     GQQGGYDWQAIRQQIGPTRTVTLKVPYPLLDALSHVNLWLSRIMSYSPMLTPGKVREL
                     TYMGWTCSNEAFTDATGWVPRTRLGQGVAAMFAR"
     gene            14622..14873
                     /locus_tag="AC16QF_08930"
     CDS             14622..14873
                     /locus_tag="AC16QF_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR6448723.1"
                     /translation="MSASQTDIIVTLAELLCAQLPEPVAVAPDTDLISDLALESVQIM
                     EFIVEVEDHYDIAISLEALAEIRNLDQLARLVITALDAQ"
     gene            14870..16057
                     /gene="spt"
                     /locus_tag="AC16QF_08935"
     CDS             14870..16057
                     /gene="spt"
                     /locus_tag="AC16QF_08935"
                     /EC_number="2.3.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020797731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004758 - serine C-palmitoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046513 - ceramide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine palmitoyltransferase"
                     /protein_id="HIR6448724.1"
                     /translation="MSLLEKFDALETTRQELLGHGTNPTDVIIERVISATESVIGGKP
                     TIMAGTNNYLGLTFDPECIAAGKRALDESGTGTTGSRMANGNYASHGELEREIADFYG
                     YPYAMVFSTGYAANIGTMAALVGPGDAVMLDGDAHASLFDGSKLSGATVYRFKHNDAE
                     SLDKRLRRLGDVASRTLVIVEGLYSILGDRAPLKELAEVTRAHDACLFVDEAHSLGVL
                     GETGRGRVEEDQVEADFVVGTFSKSLGSTGGFCVSPHRALTLFRYASRPYMFTASPCP
                     SVIATTRAALAQMRKRPELRASLWRNAETLYSGLAQLGFTLGPEPSPVVAVRMTSREQ
                     ALANWQQLLDNGIYINLILPPAAPDGTALLRCSVSAAHTPEQIQRIIDAFRQLPPDPG
                     QPA"
     gene            complement(16020..17333)
                     /locus_tag="AC16QF_08940"
     CDS             complement(16020..17333)
                     /locus_tag="AC16QF_08940"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR6448725.1"
                     /translation="MNESVSDSPVRRALKNFSVLMGGRAVAGVLSVVSLTLVARFLGP
                     VEFGLLVVVHTTAVVVRGLLNFKPSDTVVRYGVAPFDDDDHRELNHLLRFTLALDVAT
                     ASLAALCMMLVMLLAGPALGLPEELAMPAVLYGLVLLASGSGTARGVLRVAGRFHAMG
                     VQQNVGPLIRLAGVAAVYLAEGGLLGYLMVWAVAFAAEHLFMNVRGWLELKRRGVRPG
                     RPTLAGAKRRFPGLWGFVGTLYWQSNLDLAQRHGLILLAGVLLGPAGAGMFRVAREFA
                     DVLAKPVIVVRQAVFPDFARLWRERDPRFKTLYWRLGLYGGLIAAVVVGLVGLYAGEL
                     LEALVGEEYVHGAALLVWLMVAGALELIGAALRPAAYAMGAATGALRVQIVTAVCHVS
                     AFAVLVPWLGLAGAGIAAACSGAIALAGMVALVLTRADQDPGAAA"
     gene            complement(17383..18300)
                     /locus_tag="AC16QF_08945"
     CDS             complement(17383..18300)
                     /locus_tag="AC16QF_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448726.1"
                     /translation="MRDARSVIVERTAPAGLENDQGVERMLSELRARCGEHLQAVLLY
                     GSYLRGVRDTLLDFYVVVDGYRGALGSPVSALGARVLPPNVYYLAVGEGAERVRAKYA
                     VVTMRQLRRQAASGLHPYFWARFAQPARIVYARWPADEAALVQIIEQCVRTFVKRVAP
                     TLRGDFTSREFWARGFALTYRSELRSESAGRSDSIIDHDPDYYDNMLAAVADGRHLVC
                     AGGRYRAVRPRVSAAVEWACVIALGKFLSVGRLVKGALTFDDALDYILWKVERHSGVR
                     VEASARQHRYPLIFAWPLVWRLYRRGAFR"
     gene            complement(18313..19497)
                     /locus_tag="AC16QF_08950"
     CDS             complement(18313..19497)
                     /locus_tag="AC16QF_08950"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR6448727.1"
                     /translation="MSIYAHLLGDSDIELWGLTSRERLRRQVVELPDAVLVDQLGAVP
                     EDATVICIRADYLFESRTIKALAERGRAVLVCPNDGEAAALVSTGREAAQLVPLLEGA
                     TTDHDLPTLAPADLAAYEDRLRKAEEPLLLPIAEEDRGELEDRLYGRSYKGITDLVTK
                     WLWPRPARHAVRVCARLGITPNMVTLAGLLLVVYAGFAFYQGDYWLGLAAGWFMTFLD
                     TVDGKLARVTVSSSRIGHVLDHGMDIVHPPFWYVLWGMSLDDSGFSVPLLEYYWWIVA
                     GYVGGRLIEAAFHGLGSCSLFDWRPFDAYFRLVTARRNPCLILMLVAMLLGSPGGAFL
                     AVVFWTVGTTVVLMIRLLQATLARMSDGPLSSWLTEPSAATAYPRAFRTFSRTRGAYV
                     QR"
     gene            19619..20530
                     /locus_tag="AC16QF_08955"
     CDS             19619..20530
                     /locus_tag="AC16QF_08955"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6448728.1"
                     /translation="MRLIHLSDPHLSSLHGVKLRDLLGKRTLGYQSWYRKRRHQYTMD
                     TLRRVTRAVRNEAADAIVVTGDLVHLGMAQELRQAREWLESVGGPDRVLVIPGNHDCY
                     HRQSWALVKETIGPYLALEKDTADARDGYPLVRRSGEASVIAAGTAEPTAWWSASGSL
                     GAAQTTRLEDALIKARETFTFLAIHHPPLPGMAPRRKSLRDAHALAELLERHAPDVVL
                     HGHLHANQENSDTPTRIFCTAPPSSERPRAPASYRVFDVVEDAAGYRVVMRLKSLIGE
                     EMVVADEQSWTSGRSLDPGERDDAAKQ"
     gene            complement(20489..21556)
                     /gene="lptG"
                     /locus_tag="AC16QF_08960"
     CDS             complement(20489..21556)
                     /gene="lptG"
                     /locus_tag="AC16QF_08960"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HIR6448729.1"
                     /translation="MKRVDRYLVGVLLLGAVPVLLLLLGLFGFVTLAEELEDVGKGQY
                     EEADAIRVALLTLPRIAIDLLPVAVLLGGVMALGNLAQEQELTALRAVGLSQWRMAYP
                     LALAAIVLSYVVLLAQQFVVPRFEEQAKELQAKSLVNTMRGGETTEFWTRDGTHFIRV
                     GDVRFGMIPADVEIYDLGDRGVVQRVLQAEYADVIGPGQWLLHGVEERRILGKEVRRT
                     GRDTYEWESFLSPDQMASLIAPMETLTLGDLYRYVEYLEASGLNAHRYRFRLWQVLSS
                     PIALMAMCLLGLPFVLGSVRARPAGARVLFGATVGIGFYLVEQTSAQLALLYELPVIP
                     LAMLPDSALLLGSIVALTRVE"
     gene            complement(21553..>22266)
                     /locus_tag="AC16QF_08965"
     CDS             complement(21553..>22266)
                     /locus_tag="AC16QF_08965"
                     /inference="COORDINATES: protein motif:HMM:NF015684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR6448730.1"
                     /translation="GRPWAYEHLYRLEVEAEATSELERIKPGRFYLYDDNARTVFIEE
                     LKAEDDEIQGLFVRALDGDDLEIISAPSGTVSEYVSDKNHQVDLRDAQVYRKTAQGAD
                     FLGIFGELRIFVPVAAVQPVGYKPKSQNSFVLRESDALVDQAEYQWRLSAPVSTILLA
                     LLAVLLGRSRPRQGRFARLTVAVVIYAVYFNVLGIGRTWVEQGTAATLWWVPATFAGG
                     IVLAYVPWARWFRRRGRSA"
BASE COUNT         4054 a         7548 c         7119 g         3545 t
ORIGIN      
        1 gaagtcacct cggactacgg ggagtttctg cacgccgagc tcgccctgga cggcgccgac
       61 ccctacgctt cggagggcct cgacgagatc tccgaggcgg cacggcggga gttcaaggtc
      121 atcatcgtcg gcgcagggat gtcggggctg ctcgcggcga tcaggctcaa ggaggctggc
      181 atctcctggg agatcgtgga gaagcacgcc gacgtgggcg ggacctggta ccagaacggc
      241 tatccggggt gtcgggtcga cagccccaat cacatctact cctacagctt ccggcccgcc
      301 gactggcccc agtactactc gagccaggaa gttctgcggg agtatttcga caaggtggcg
      361 acggagtacg gcctgcgcga ccagatccgt ttcagcacgg aagtgcagtc catggcgttc
      421 gacgatgcgt ccgggacgtg gcaggtggaa gtcacgaacg gtaccggcgc cagggagacc
      481 ctcaccgcga acgccgtcat cagcgccgtg ggccagctga accgaccgaa atggcccgac
      541 ctcgccggcc aggatcgttt tcgcggcgtg tcctttcact ccaccgagtg ggaacacgag
      601 cacgatctgt ccggcaagcg catcatcgtc ataggcaccg gtgccagcgc gtttcagttc
      661 gctcccgaaa tcgccaaggt ggcagccgag gtcgagattt ttcagcgcac acccaactgg
      721 atggccccgg tggccaccta ccacgaccac ataccgctgg ggaagcactg gctcctcaac
      781 cacgttccct actacgcgaa gtggtaccgc ttcgccatgt tctggaacat ggccgagggc
      841 gtgctctccg cggtgcgcaa ggatccggcc tggaaccaga ctgaagacgc catcagcgcc
      901 gaaaacgaca cgctccggca gatgttcacc gactggctcg aagagctgtg tgaaggcgac
      961 gaggaactgc tcgaaaaggt ggtgcccaag ttcccggtgg gcggcaagcg ggtgctgctc
     1021 gacaacggca actggattcg cgccttgagg cgcgacaacg tgcacctgct caccgatccc
     1081 atcgccgaga tcaacgagac cggcgtcgtc accgaggatg gcaaacagca cgacgccgac
     1141 gtgctgatct acggcaccgg cttccaggca agccggtttc tatggccgat gaaggtcacc
     1201 ggtcgcgacg ggatcgacct gaacgagcag tggcagggag acgcccgcgc ctacctcggc
     1261 atcaccatcc ccaacttccc caatcttttc tgctgctacg ggcccaacac caacatcgtc
     1321 gtcaacggca gcatcgtctt cttctccgaa tgcgaggtgc gctacatcct gagctgtatc
     1381 cgccagctgc tggccggcgg ccacaaggcc ctggattgcc gcgcagacgt tcacgatgcg
     1441 tacaacgagt ggatcgacga gggcaacgcc aacatggcct ggggcgcggc ccgggtaccc
     1501 tcctggtaca agaacgagaa aggccgggtg tcccagaact ggcccttcac gctgctcgag
     1561 tactggaacc acaccaggga cgtcaacgcg gcggactacg agttcctctc cgacgcgtga
     1621 cgcgccccgc gctcgcagaa gccggcgcgc gacgttaaga ttgcgcgctc gcggcgcttc
     1681 gggtcgttca aggaggttcg gtgattctcg atctgcacac acattccatc aagtccgatg
     1741 atggtcgtgc caaggtgcag aactactgcc agtggatccg cacacgcgag atccccatcg
     1801 acggcctcgt gctcaccgag catcgccagt tcgacgccga gtccgactac tccccgctgg
     1861 ctcagcagtt cgggatcaca atcctcaagg gcagcgaggt cgagaccgag tacggccacg
     1921 tcctggtgtt cggcgtgacc gaagcgctga cccgggcgtt cgatttcgcg gacatccacc
     1981 ttcctttggc ccgggtcatc gaagcctgtg cgctgcacgg cgcggttccc gttccctgtc
     2041 atcccgggcg ccggcgcgtg ggcatgagcg ctcatctcga ggaattcggc acgccggaag
     2101 gggtgaggat cgtggagacg cacaatggcg gcagccgtgg agacgaggac gccgtggccc
     2161 aggccatggc cgccgagctc ggctacctgg gcattggcgg cagcgacgcg cacatcgtca
     2221 gccacgtggg acgctgcgct acccgtttcc caggcacgat ctccaacgag caggaactgg
     2281 tggaagcgct gctggccgaa gagttcgaag ccgttaccaa tcgggagtga aagacaccca
     2341 tgcttgacct agaggcgtta cgggcaaact tcctcaacga agacttcgac gagcagcact
     2401 tcgaggtgaa cgccgagcag tgcgttgcct acgccctggc ctgcggcgag acggcgccgc
     2461 gctacaccga cccggcccat cctgacttcc aggcgccgcc cacgctgccg tcgagctttc
     2521 accccggccg ccgcctgcct gacggctttc ccaaactacc cggtctgggc atggacgcag
     2581 gcaaggctgt tgcgccgaag cggcccatac ggcccggggt gccgctggtc gggcgcacgc
     2641 atctccacga cgtttacgcc aaaaccggac gatcgggccg catgacgttc atggttacgc
     2701 gcatggagat ctacgatccc gacggcgagc agctggcaac cgctgacacc cgaatcgtca
     2761 ttcgagagag gccggatcaa tgaccgtcaa cgtcatcggc gacgtcgagt tcggcgaaga
     2821 gctaccggtc ttcgagccgg acacgagcct ggccaacgtc aagcgattca ccgaagccgc
     2881 cggctggcag ggaccccggt tcacggacca tgaggccgcc aggcgcgagg ggctccccgg
     2941 agcgctggtg ccaggcatca tgagtcaggg ttttctcgcg gccatgatcc atcgctgggc
     3001 gccggagagt cagatcgaaa gcgtggacac cgtgttcagg gcgcccgtgc tggttgacga
     3061 aaggcacagc atcagcggcg tcgtcaccga catcgacgaa gatgcgggca cggtcgaaat
     3121 tgatctgacc gtgacgaacg aggccaacga gacccgggtc ttcggcaccg ccaccgttcg
     3181 cctccccgcc tgatctcccg ccggatccgc cgatcacaac cggcaaaggc ggcagcaacc
     3241 ggtcgttcag atcggctcga aaaggggcac agccagctct tcgtggtctt cccacgcgac
     3301 gcgcacccgt tggcccaccc gaatgtcctc cacggggccg atcacgttgg acagtagccg
     3361 cggcccctcg tccaggtcga tccaggccac cgcgtagggg accagagcgc ggaagaaggg
     3421 gtgatagctc tgcctgatca cgctgaacgt gtagacggtg ccctccccct tggccgtttt
     3481 ccaggccagc gcgtcgccaa gacaacccgt gcaatgccgc cgcgggtaga agacgacggt
     3541 gccgcagtcc gcgcactcct gatagcgcag ctcgtgttcc cgggtggctt cccagaatgc
     3601 ccggctgtcc atttcgtgca ggttcggcag cggtctcttc gcttcgctca tggtgctact
     3661 cctcaatccc gcccaagtat cacggtaccc gcgcaatgcc ggctgccgag catgccgccg
     3721 ttgccgtggg ccacggccag cttcgcaccg tccacctgag agcaggaatc accgcgcagc
     3781 tggcgcgcgg cttcgaccag aagaaatatg ccccgcatgc ccggatggtt cgacgagagc
     3841 ccgccgccat cggtgttcag ggccgggcca tccgcggggc gatcgaagcg caaacgccct
     3901 cccgcggccc agggcccgcc ctcccccttc gcgcagaatc ccagatcctc caggatcgtg
     3961 agcaccgtga tgctgaacga atcgtagatc atggcgatgt ccatctcggc cggagagacg
     4021 cccgcctcag caaaggcctg cggcccggac tgggccgcgg cgctcaccgt tatgtcgccg
     4081 ccattttcag ggtatttggt ggcttctccg gtgcccagga tccacaccgg cggacgcgcg
     4141 cagttacggg cgacttccgg cgaggcgatg accacggctc cgccgccgtc cgagatcatg
     4201 cagcactcca gcagatgcag cgggtcggcg atcatcctcg agccgaccac gtcctccacc
     4261 gtcgtttcgc gaatgtcgag gaactcgaga tcctccatgg ctttgacggc ctcgggatta
     4321 cgcatggcgt gacagcgggt cgccacggat atttctgcca gctgctcgct cgtggtgccg
     4381 tactcataca tgtgccgctg cgccaccatc gcgtagttgg ccaccagggt catgccggca
     4441 aaggcatcca tgttgtccgc cggatgcatg ccgcccccac cgcgcccgcc aacgccgatg
     4501 gcccgtgcgt tgctgtgcgc tgtcgatccg taggtcagca gcgccaccct cgccttgccc
     4561 gccgcgatgt ccctggccgc gtgggcggcg tggtactcgt aggagctgcc gcccacattg
     4621 gtggtgtcga tgacgttggg tttaagcccg aggtactcgg cgatggtgag accgctcatc
     4681 atcccggcct cacccgcgtc gtagatggcg tcaacgtcct gccagctcag cccggcatcg
     4741 gcgagcgccc gggcagcgct ttccgccttg atctgcaacg ggctgacgga tccctcgacg
     4801 tcgcgcgaag ggtactcgtg aatgccgacg atggcggcgc ttctggcttg accagccacg
     4861 gctatctccc ccaggttgat gcccgcagtc taagccgtgc gggccgacgg tccaaggccg
     4921 gtccttgcgg gctatgcggc gatgcgcacg ggcaggctct gaatggcgcg gaacagcacc
     4981 gagctggcac gctcaggctc cccgacaacc tcgattcgag ggcatcgttt caggatttcc
     5041 tcccagagca ccctcagctg aagctcggcc agacggttcc ccagacagcg gtggatgccg
     5101 aacccgaagg aaaggtgctc acggggccgc ctgcggtcga tgatgaactc gtccggccgc
     5161 tccacggcgt ccggatcgcg gttccccgat atgtaccaca tgaccacctt gtcacccttg
     5221 ccgatcagct ggccacgaaa ctccgtgtcc gccagcgccg tgcggcgcat gtgcgtgagc
     5281 ggggtctgcc agcggatgat ctccggcacc atcgtgcgga cgagatcggc gttctccagg
     5341 agcttgtcgt actgctccgg gaactggttc agggcgagca ggccgccgct gatggagttg
     5401 cgggtggtgt cgttgccgcc cacggtcagc agcactgcgt tgcccagcag ctcctgggaa
     5461 ttcatgtcgg cagtgtccgg cccgtgggcc agcatggaca ccagatcgcc cgtgggcgct
     5521 gacgacttgc ggtcgcgcca gagggcgtcg aagtaatcgc cacactgcgc gagggcttcg
     5581 aaatacgacg acttctcctt gaccacctcg gggttgtccg cgttgctgat gatgttcgac
     5641 cagcggatca gctgatggcg ctcttcctgg ggcacgccga acagcgttgc gagcatctgc
     5701 gcggtcagct cgatggaaac gtgctccacc cagttgaacg tctcgttgcg gggcaggccg
     5761 tcgagaatcc tgcccgcgcg ctcccgcacg gtggcctcga ggtcgccaag gttgcgctga
     5821 gtgaacttcg gggctaccgc tttgcgttgc gcgtcgtgtt tgggcggatc catctggatg
     5881 aacatcggca cgtcgttgcc ggaggacggc gtgcccatga gcgttacccc gcccagcgag
     5941 aactcggagg agaacaccgc gtggttcttc tcgatctgca tgatgtcgtc gtagcgggtt
     6001 atggaccagt acggtccgta ctggctttcg gaacagtagt gcacgggcgc ctcttcccgc
     6061 agccgtcgga agtagggcca gaacgcctcc gccctgaaca gctcggggtt ggcaacgttg
     6121 agatcctgga gcgggatctc gtaagggtcg agctgcggat cgaagggaat ccgctgccgg
     6181 cgtcgcgtgg gcgtcgttgg cgccagcacc gccttttccc gcagatgatc gtaccgagat
     6241 tgctctttca tgaccttcgc tgaccaatcg ccgaccccgc catcatatca ggaggccgga
     6301 tgggttgcgc ccgagagatg gagcccgctg caatcggtca ggcggcggca gagcgcggcg
     6361 aggtcccggt ggccgttcac cgacgcgcag agattgacgc ctgcaagggg ctcgggcgtg
     6421 gccgcgagca tggctcgcag cgcttctcct ccgccctgca ccacgtcgtc caggacggcg
     6481 ggagacagag caccgatggt atcgatcagg ccgaatccgg tcaggttgtg cagggcggaa
     6541 agcagcttgg ggcccaccat ctgcggggcg atgtgctggg gatccgcacg ggagatgatc
     6601 ctctgggtcg cgtgaatcag gaagggcaga acggggcagc cggcaacgaa ggagcacaag
     6661 gcgttgtgca cgttgcgacg gacctgccac cgcccatcgc gattcttctg gacccagtac
     6721 tcccggccga aggcgcattc gtcgtcatcg ggcaggtcga ggcgttccgg tgcgtacagg
     6781 agcacgtcgg catctatcca gcaggcacgc tcatagccct tggccaacgc gtcgcgaatc
     6841 agcagcagcc tcgccaggtc cgcgacgatg ggagagcgcc cgatgagctt ggcgcgatac
     6901 caccccggcg tgagctccag cgcatcgtcg ccgcggaagc gatagtcgta gccacgctcc
     6961 cgcgcccagc cttccacgct ttggaggcac gacgacagcc aacccggccg gtccagctcg
     7021 ctggcgctct ggatgacaag tgtccgcacg cgtatcagtt gcgcacgcgg ccgatgggtg
     7081 ctgccggcac ggaaacgctc acgtcagcta aaatcctttc gtctcattag aaacgtgaag
     7141 cgagtggact ggcaagcgaa gctcaacgag ggcggcatcg tcctgctgga cggaggcacc
     7201 ggctccgagc tccagcgcag gggcgtgccc atgagcggcg cagcctggtc gggggcggcc
     7261 gcgcacaccc atcccgacgt gctgcgcgcc gtgcatcacg actacattgc agccggcgct
     7321 gaggtgatca tcaccaacac cttcggaaca tcccgggcca cccttcgagc cgccggcctg
     7381 ggcgatgcgt tcgcgtccat caaccggcgc gccgtggccc tggccatgga agcgagagag
     7441 ctcgccggtc gcgacgtcgc catcggcggg tcgatatcca acctcccgcc caacatgaac
     7501 atcggggact atcccgaccc ggccgaggaa ctggccgatc tgcgcgatca ggctgccatc
     7561 ctggcggagg gcggcgtcga tttcatcgcc ctggagatga tgcaggacgt gaaccacgcg
     7621 ccaagagcga tgcgcgccgc ggtggcaacc gggcttcccg tctggttggg cgtcagctgc
     7681 cggagatcga cgcaaaccgc gagcctcgtg gctttcgact tcgctgacac gccgttcgaa
     7741 gcgccgctgg acgcgctgat cgccatgcgt cctgcggtgg tcaacgtcat gcacagcgag
     7801 gtcgaggcga ccgtgcccgc catcgaggcg gtccgcgaaa gatggcccgg ccccatcggc
     7861 gcctatccgg agatcggcta cttcgcggcc cccgaccggc gcttcggcga cgatcacccg
     7921 ccggaccgtc tggtgtccga ggctcgcagg tgggtggcgg cgggcgctcg attgattggc
     7981 ggctgctgcg gaaccacacc ggatcacatc cgggcgttgc acgctgcgct gcctgagctc
     8041 gaaaggctcg ccggggagac cgagacgccg ggccgatcgc ccaccgtccg gtgatggtct
     8101 ctccaacgca gaacggtcag gcgcgggggt cgaccagaat cttgatctgc tccggctcgc
     8161 tggcgagctc ggcaaaggct cccgccatgc ctgcgaggct gaccgtcgag gtatgcaggg
     8221 gaccgcagtc gacgcggccg tccgcgacga ggcccttgga aacctcgaag tcgtcccgca
     8281 cgtagccgag cgccgtggat accttgagct cctgtgcgag ccattgcgcc gcggaaatct
     8341 gcgacaaccc ggtggggtaa cccaccagcg ccacggtgcc gccccggcga acgtaggcgg
     8401 ggacttcctc cacggtgcgc gccagccccg cacactcgaa cacgacgtcg gcgcccgact
     8461 tgccgatgat cgagttgatg gcagccgcca cgtcgggggt ctcccgggga tcgatggtgt
     8521 ggtcggcgcc cagcgctgcc ccggtttcgc gccgggtagc ctggggttcc accagcacgc
     8581 agacacccgc gccggcggcc cgagcacact gcagcaccag caggccgatg gggccggcac
     8641 ccaccaccac cacgctgtcg ccgagccgga taccggtgcg gcggaccgca tgaacggcaa
     8701 tggtgaccgg ctcgagaatg gcagcggcct cgtcgcttat ttccgggcct acgtggtaga
     8761 gacgagccgc ctcgatggca atcgccgagg cgaagccccc gtggggggcc gccattgggc
     8821 cgattccgac catgccggca aaggccatct cgcagtggga cgggtctccg cgcacgcagg
     8881 tggcgcactg gccacaggcg gttgccatgc cgatggccac gcgatcgcct tccttgacct
     8941 gcacgccggc gcctgccgcc gagacatggc ccatccactc gtgcccgcag atggccgggt
     9001 tgtaaggctc gcccgactga taggcatgaa cgtccgtccc gcagatgccg caataggaaa
     9061 cgtccaccac cgccttgccc ggctccgggc ggggatcggg catctccacg agatcgaccg
     9121 tttccttacc cgtaactaac ccaacctgca tggccttacc cccgtttcga attgcgccaa
     9181 aggatacgcc agtcggcgcg cagcggcatc cttggacggt tcaggcgccg ccgaggaacc
     9241 gggccgtttc gcgatatacg tgtcgggcgc gctccgcggg atccgggaaa cgctcgcgca
     9301 ggggtcggga gcgcagctcc aggctgagtg gcaggtcgtc gggcaggacg tccagaagat
     9361 cgcgcagggg cagttgaccg gcccccgggg tgaaccggcc gttgacggcc tcctcccgaa
     9421 gaacgtccag ggtaggctcg cctggaggct ctttggcccc atcgcagaac tgagcaaagc
     9481 tgaggagctc gccgggcaca gcggcgatgt ccgcaacgct ggcgcccgac ctggcgacgt
     9541 gcagaggatc aatgaggagc ttgccgcgcg gatgtcccac gtcccggatg atccgcaggg
     9601 cggaaggcag atcccgaacc gcgtagatcg gcagaaattc gaggcagacg ttgacgccca
     9661 gctgctcgcc aagctcgcag agatgggcga atcggcggca gacggccacc tcgtcgggat
     9721 cgctcgagac cacgatggcc tcggtggccc caaccgcagc gccgctctcc agcaaacgct
     9781 gatgttccgg gtccggtgct cccggccgga tgtcgatgac ctcgatttcc agcggcgtca
     9841 gaccggtgtc cgcaaaggcg gccgccacgc cgcgcgtggt ctcatcggtc cacgtggcgg
     9901 cgtcgaacca gataccgcag ccgttgaacc cccctgcggc agccgcccgg gtgacctcgg
     9961 caggaccgaa ctcgggcagg gttcccgatg ctagagatat gagacgcgcc acgatgatcg
    10021 tcctgattgc tgcctgggcc agtgtgcttc atttgcagcg ttctggcgag caggcccgct
    10081 accccatagc tcccgaggcc gggccgccgt caggcactgg gggcgactcg acgggaaggc
    10141 aggattcctc cgggcgcagg ctccccgggc gctcaactcg gatctgcaca tcttccccgg
    10201 ggcgatttgc cgacgcggcg tggaccgtcg cgggccggat gttaaagtgc gccccgcccg
    10261 cgcgccctca aacaggagcg agactggatc gatgcgactt gtcagctgga acgtcaacgg
    10321 cctgcgcgcc tgtgcgaaaa agggctttct cggatttctc gagacctccg gggccgactt
    10381 cgtcgccctg caggaagtgc gcgcgttccc cgatcagctg gacgacggcc tgcgagcccc
    10441 gcccggctgg cacgtttgtt tctctcccgc ggagcgcccc ggctacagcg gcgtcgccct
    10501 ctacgcccgg gccgcccctg atagggttca gaccagcctc gggaagccgg acttcgatcg
    10561 ggaggggcgg ctgctcagcg cgcatttcgg ccgcctggcc atcgtgagcg tctacttccc
    10621 caaaggcagc ggcaaggatc gggacaacag ccgcgtcggc tacaagctcg atttttacgc
    10681 cgccgtgttc gatcacgttg agcagctgcg ccggagaggt cccgtatttg tggttggcga
    10741 ctacaacacg gcccacgagc ctatcgacct cgcccgtcca aaaaccaaca ccaagtcgag
    10801 cgggtttctg cccgaggagc gagccgagat cacgcgctgg atcgacgccg gctgggtgga
    10861 cgtcttccgc cgccagcatc cggggcagcc cgaccactac acgtggtggc ggcagtgggg
    10921 caacgcccgc cgggataacg tcggctggcg aatcgactac gtgctggcct cgccatcggc
    10981 tgcaaggcgg gtcaggcagg cgttcatctg gcccgaggtg accggatccg atcactgccc
    11041 ggttggcgtt gacttcgaat gagacgacgc caccgggccc gcattgaccc gcattgaccc
    11101 gcattgcctc gagcgcatcg gatttctata gtccacccat cgtccaggcc agaggcagtc
    11161 tcccgtggca gacgcacgtt gctggaattg tggtacgtcg atcgacgacg tgcccaaacc
    11221 cataagccgg caagcaacct gttctaagtg cttccacgaa ctgcactgtt gtcgcatgtg
    11281 ccggcactac gatgagtccg ccagcgcggg aaggtgccgg gaggaacgtg cagatccccc
    11341 tcccgaaaag gacgttgcca acttttgtga ctggtttcag ccaggcaaga acgccttcca
    11401 ggaaggccgc accacggcgg gcgagaatgc ccgctcacgc ctcgatgcgc tgttcggcga
    11461 ttcgcagggc ccggcggaag agcctgacga ggcgccgcca aaacccgagg atgggccccg
    11521 aagcgccgag gagaaggccc gcgccgagct cgatcgcctg ttttcgcggg acgctgactg
    11581 attgcgcccc gcgtcggata tcgctccgag gcgagatggg cccgaccagc gacatcgcaa
    11641 atctgtgaca ttcacacgcc atttccgtga aacgcacccg ttatcgcggg tctacaatcc
    11701 gtaggtctct gatttcgttt gaaaagacac tatcgctctc agacgaattt gcaggtagac
    11761 tgcgccacca gaaatataag tgccggatga ttcgccggcc gatggagagc ctcggtttgt
    11821 tgggcagctg tgttcggagg ggttcgagca caacgaaaag agcaaacacg acaatttgag
    11881 ctggttatca catgagccct accactcgtt gtagcgggat atcgcaccgc acacgtggct
    11941 ttatgagcct ggttgatgcc ctggagtacg ccgcgcaagg cgacacgggg ctgaacttct
    12001 atgatggctc cgggagcctc agcgccgcgt tgccgtacgc gcagctggcg caggacgctc
    12061 ggatcctggc gacccggctg ctcggcctgg gcgtcaaacg cggcgcccgg gttggcatcg
    12121 ttgccgaaac cgaccccatg ttcccgcgat tcttctttgc ctgtcagtac gccgggttga
    12181 tcccggtggc tctgcctgcc ggtctgcaga tgggcggcgg cgaggcctac gtggagcagc
    12241 tcagtcgcat gctggaaagc tgcggagctg cggtggcggt cgcgccggaa tctcacctca
    12301 gcttcctgaa gcgtgcgacg cgacagctgt cgctgcgcat gtccggatcg ccagaggact
    12361 tcgacgccct gccagcggtg ccggtgaagc tgctcccctt gagtgccgac gaacccgcct
    12421 acctgcagta cacgtcggga agcacccgct tcccccgcgg cgtcgagatg acccagacgg
    12481 ccgtcctcgc aaacctgacc gaaatcgccg agataggcac ccgcatcacc gccgacgacc
    12541 ggatcgtttc atggctgccc tactaccatg atatgggcct ggtgggttgc ctgctggtac
    12601 cggtggcaac gcagatttca gccgacttcc tgtcgccgcg cacgtttgcc atgcgtccac
    12661 ggctttggct gaagctgctc tcggaaaatc gcgggacgat ctcgtccagc ccgccgttcg
    12721 gctacgagct gtgcgcgacc cgcgtccgca tgacggacac ggagcgatac gacctcagcg
    12781 cctggcgcgt cgcctgcgtc ggcgcggagc gcatcaaccc gcgtccgctg aagcagttcg
    12841 ccaacgtgct ggctcctgcc ggcttcaacc ccaaggcgtt tctgccctgc tacggcatgg
    12901 cggaggtggg tctggcaatc agcttcgccc cgctggagga cggctacttc gtcgacgacg
    12961 tggccaaggc ctcaatgtct gcggaaggcc tggccatcga ggccaccgag ggcgaccgtg
    13021 cgaagggcaa caccctctcg ttcgtcgact gcggcgcggc gcttccaagc tacgacgtct
    13081 ccatccgcga cgagtcgggc cgagagctcg acgagcgcga gtgcggacgc atctgggttc
    13141 gggggccaag cctgatgagg ggctacttcc gcgatcccgt gaccaccgcc gaggtcatga
    13201 agcccggcgg atggctcgat accggcgaca tcggttaccg catcggcagg cgtctgttcc
    13261 ttacctcgcg ggccaaggac gtgatcatca tcaatggccg caacatctgg ccgcaggatc
    13321 tcgagcagct cgccgagcgc ctgcccgagg ttcggctcgg ggcgatttcc gcgttctcgg
    13381 ttacccgccc gaatcaggaa gagctggccg tgatggtggt ggagtcccgc aacgccggtc
    13441 gggagctcag cgacgagctt tcgggcatgg tgcgggagca cttcggcatc aactgttaca
    13501 tcgagctggc accgccgagg acgctaccgc gcacctcgtc gggcaagctg tcccgcgcac
    13561 gcgccagggc cgacttcctg gatcgcacgg cctgggatgc cgacggttat cccatgacgg
    13621 cgccggaagt ccaggcacaa gcgggctgac tcacccaggg tgatctttgc ctgataaccc
    13681 tacccgcccg ctcatcgcgc tgacgggcgc caccggcttc atcggccggc atctgcagcg
    13741 cgcgctgctt gatgcgggct gcgccgtgcg agcgctggtt cgaacccgct cgcttgcgag
    13801 ctcggggctc atcgaaggcg tcgaagtcgc agaagccgac ctcggctgcg aatcttccat
    13861 gaagctggcc ctgaacgacg tcgaccagct ggtttacgcg gccggatcgg ttcgcgggcg
    13921 ggatcccgag gacttcgcgc tcgccaacgt caccggcgtc aagctggccg ccacggcgct
    13981 tgcctctcgc aagccgggca tccccatgct gctcatttcc tcgctggcgg cgagcgcccc
    14041 cgagctgtcg cactacgcgg ccagcaagcg cgcgggagaa gacgctctgc gaggaagcgg
    14101 gctcaccagg tgggccatca tgcggccccc agccgtctac ggacccggcg acagagagct
    14161 caagggcacg ttcaccgcaa tccggcgcgg cctggtgccc atggtcgctc cggcagacca
    14221 gcatctgcct ttcatcgagg tcggcgatct ggccgatgcc gtgaacgcct ggagccaaca
    14281 tccgcaagcc tgctcaggcg ggacctttgc cctgcacgac ggccagcagg gcggctacga
    14341 ctggcaggcg atccgccagc agatcggtcc gacccgcacg gttacgctga aagtgccgta
    14401 cccgttgctc gacgcgctga gccacgtaaa cttgtggctt tcgcgcatca tgagctactc
    14461 gcccatgctc acgccgggca aagtgcgcga actgacatac atgggctgga cgtgcagcaa
    14521 cgaagctttc accgacgcca cgggatgggt tccgcgaacc cgcctgggcc agggcgtcgc
    14581 tgccatgttc gcccgttgaa cgaccctgag aggtattgca cttgtccgct agccagaccg
    14641 acatcatcgt cacgctcgca gaactgctct gcgcccagct tccggagccg gtagccgtcg
    14701 cccccgatac ggatctcatc tccgatctgg ctctcgaatc ggtccagatc atggagttca
    14761 tcgtcgaggt ggaggatcac tacgacatcg ccatcagcct cgaagcgctg gccgagatcc
    14821 gcaacctcga tcagctggcg cgtctggtca tcaccgccct ggacgcccaa tgagcctcct
    14881 ggagaagttc gatgccctgg agactacccg ccaggagctc ctcggccacg ggaccaaccc
    14941 gaccgacgtg atcatcgaga gggtcatctc ggcaacggaa tccgtcatcg gcggcaagcc
    15001 cacgatcatg gcggggacga acaactacct cggcctgact ttcgacccgg aatgcatcgc
    15061 cgccggcaag cgggcgctgg atgaatcggg caccggcacc acgggatccc gcatggccaa
    15121 cggcaattac gcttcccatg gggagctgga gcgggagatc gccgacttct acggctaccc
    15181 ctacgccatg gtgttctcga ccggctacgc ggcaaacatc gggaccatgg cagctctggt
    15241 gggtccggga gacgccgtga tgctcgatgg cgacgcccac gccagcctgt tcgacggcag
    15301 caaactttcc ggcgcaaccg tttaccggtt caagcacaac gatgccgaga gcctggacaa
    15361 acgcctgcgc cggctaggtg acgtcgccag ccgcacgctg gtcatcgtcg aggggctcta
    15421 cagcattctg ggagaccgcg ctccgctcaa agagctggcg gaggtgaccc gcgcccacga
    15481 tgcctgcctg ttcgtcgacg aggctcactc gcttggcgtg ctcggagaaa ccggacgcgg
    15541 acgggtggag gaagaccagg tggaagccga cttcgtcgtc ggcaccttca gcaagagcct
    15601 gggttccacg ggcggcttct gcgtgagccc ccaccgagcg ctgaccctgt ttcgctatgc
    15661 cagccgcccc tacatgttca cggcgtcccc atgcccgtca gtcatcgcca ccacgcgggc
    15721 ggcccttgct cagatgcgca aacggcccga gctgcgcgcg tcgttgtgga ggaacgctga
    15781 aacgctctac agcggcctgg cccagctggg attcaccctc ggccccgagc cgagccccgt
    15841 ggtcgccgtt cgcatgacct cccgggaaca ggcactggcg aactggcagc agctgctgga
    15901 caacggtatc tacataaacc tcatcctgcc cccggccgcg ccggacggca ccgcgctgct
    15961 tcgctgcagc gtcagtgcag cccacacgcc ggaacagatt caacgcatca tcgacgcgtt
    16021 caggcagctg cccccggatc ctggtcagcc cgcgtaagca ccagcgcgac catccctgcc
    16081 agcgcaatcg ctcccgagca cgcagcggcg atgccggcac ccgccagccc cagccagggc
    16141 acgaggacgg caaaagcaga aacgtggcat acggcggtga cgatctggac ccgcagcgcg
    16201 ccggtcgccg cgcccatggc gtaagccgcc ggcctgagag ccgcaccgat cagctccaac
    16261 gcaccagcga ccatcagcca cacgagcagc gccgcgccgt gcacgtattc ctcgcccacg
    16321 agcgcttcga gaagctcgcc ggcataaagg cccaccaggc cgacgaccac ggcggcgatc
    16381 agcccgccgt aaaggccgag gcgccagtag agcgtcttga accgcgggtc gcgctcccgc
    16441 cacagacggg cgaagtccgg gaacacggcc tgacgcacca cgatcacggg cttggccagc
    16501 acatcggcaa actcgcgggc gacgcgaaac atcccggcac ccgcggggcc aagcagaacg
    16561 cctgccagca ggatcaggcc gtgccgctgc gcaagatcga ggttcgactg ccagtagagc
    16621 gtgccgacga agccccacaa ccctggaaat cgtcgtttcg cgccggcaag ggtgggacgt
    16681 ccgggccgga ctcctcgcct tttgagctcc agccagccac gcacgttcat gaagagatgc
    16741 tcggcggcaa acgcgacggc ccaaaccatc aggtaaccaa gcaagcctcc ctcagccagg
    16801 tacacggcgg ctacgccggc cagccggatg agcggcccca cgttctgctg gacgcccatg
    16861 gcatgaaatc gccctgctac ccggagcacg cctcgcgcgg tccccgagcc cgatgccagc
    16921 agaaccaggc cgtagagaac ggccggcatt gccagctcct ccgggagccc cagggcgggg
    16981 cccgcgagca gcatgacgag catcatgcac agcgcggcaa ggctggcagt ggcgacatcc
    17041 agggcaagag tgaagcgcag caggtggttc agctcccgat ggtcgtcgtc gtcaaacggc
    17101 gcgacgccgt atcggaccac cgtgtcagac ggtttgaagt tgagcaggcc gcgcaccacc
    17161 acggccgtgg tgtgcacgac cacgaggagc ccgaactcta ccggtccgag aaagcgggca
    17221 accagggtca gcgaaaccac ggacagcaca ccggccacgg cacggccacc catcagcacg
    17281 gagaagttct tcagcgccct gcgaacgggc gagtcggaga ccgactcgtt cacgcgcgag
    17341 ctccgccccg cgcccgcacg gcggacccgg cagtgtgacc gttcaccgga acgcgcctct
    17401 gcgatagagc cgccagacca gcggccaggc aaagatcagc gggtagcgat gctgccgggc
    17461 gcttgcttcc acccgcaccc cgctgtgccg ctccaccttc cagagtatgt aatccaacgc
    17521 gtcgtcgaag gtgagcgcgc ctttgacaag ccggccaacc gatagaaact ttcccagcgc
    17581 gatcacgcac gcccattcca cggcggctga aacccgcggc ctgacggcgc ggtagcggcc
    17641 tcccgcgcag accaggtgcc ggccgtcagc cacggcggcc agcatgttgt cgtagtagtc
    17701 gggatcgtga tcgatgatcg agtcacttcg cccagccgac tcggaccgaa gctcggaacg
    17761 gtaggtgagc gcaaagcccc gggcccagaa ttcccggctc gtgaaatccc ctctgagcgt
    17821 gggagcgacg cgtttgacaa acgtacgcac acattgctcg ataatctgaa cgagtgccgc
    17881 ctcatcggcg ggccagcgtg catagacgat gcgggccggc tgggcaaagc gcgcccagaa
    17941 gtagggatgc agccccgagg cggcctgcct gcgcagctgt ctcatggtca cgacggcgta
    18001 ctttgcccga acacgctcgg cgccctcacc caccgcaagg tagtagacgt tgggcggcag
    18061 gacccgcgcg cccagggccg aaaccggcga gcccagggcg ccgcggtagc catcgacgac
    18121 cacgtagaaa tccagcaggg tatcgcgcac gccgcgcaga taggatccgt agagcagcac
    18181 ggcctgcagg tgctcgccac accgggcacg cagttccgag agcattcgtt cgacaccttg
    18241 atcgttctcg agaccggcag gtgccgtgcg ctcgacgatg actgacctgg cgtcacgcat
    18301 caggcgaggt gactagcgct gcacataggc tccgcgggtc cgcgaaaatg tccggaacgc
    18361 gcgggggtat gcggtcgccg ccgacggctc ggtgagccaa gaggacagcg gcccgtcgct
    18421 catgcgggca agcgtcgcct gcagaagcct gatcatcagc acgaccgtgg tgcccaccgt
    18481 ccagaacacc accgccagga acgcgccgcc aggactgccc agcagcatcg ccacgagcat
    18541 caggatgagg cagggattgc gcctggccgt gaccagtcgg aagtaggcgt cgaacggcct
    18601 ccagtcgaac aggctgcagg agccgaggcc gtgaaaggcg gcttcgatga gccgtccgcc
    18661 gacgtagccg gcaacgatcc accagtagta ctccagcagg gggacgctga aaccgctatc
    18721 gtccagcgac atgccccaca gcacgtacca gaacggcgga tgcacgatgt ccatgccgtg
    18781 atcgaggacg tgcccgatgc ggctcgagct cacggtgacc cgggcaagct tgccgtcgac
    18841 cgtatcgaga aaagtcatga accatccggc cgccagaccc agccagtagt cgccctggta
    18901 gaaggcaaac cccgcataaa cgacgagcag gagacccgcc agcgtgacca tgttgggcgt
    18961 gattcccaac cgcgcgcaga cccggaccgc gtgacgcgcg ggtctgggcc acagccactt
    19021 cgtcaccagg tcggtgatcc ctttgtagga acggccatac agccggtcct cgagctcgcc
    19081 acggtcttct tccgcgatcg gcagcagaag cggctcttcg gcctttcgca ggcgatcctc
    19141 atacgcggcc aggtcagccg gcgcgagcgt cggtagatcg tgatccgtcg tcgcgccttc
    19201 gagcagcgga accagctgcg cggcttcccg gcccgtgctc accagggcgg ccgcctcgcc
    19261 gtcgttgggg caaaccagaa ccgcacgccc gcgctcggcc agggctttga tcgttctcga
    19321 ctcgaagagg taatccgcgc gaatgcagat caccgtagcg tcctccggca cggcgccgag
    19381 ctgatccacc aggaccgcgt cgggaagctc gacgacctgt cggcgcaggc gctcacgtga
    19441 ggtcagtccc caaagctcaa tatcggagtc gccgagcagg tgtgcgtaaa tgctcatgtt
    19501 atagttggaa attcactgcg cacttgggga tcctgggact tgcggcgggc gtttacaggc
    19561 caagccgccg acggtcgcgg attataggca tgccccagtt gctcggcaaa gggcggccat
    19621 gcgtctaatc catctcagcg atccgcatct ttcatccctg cacggcgtga agcttcgcga
    19681 cctcctcggc aagcggacgc tcgggtacca gtcctggtac cggaagcggc gccatcagta
    19741 caccatggac acgcttcgca gggtgacgcg cgcggtccgc aacgaggccg cggacgccat
    19801 cgtggtcact ggcgacctcg tgcatctcgg catggcgcag gagcttcgcc aggccaggga
    19861 gtggctcgag tcggtgggcg gcccggatcg ggtgctggtc attcccggca accatgattg
    19921 ctaccatcgc cagtcgtggg ccctggtcaa agagacgatc ggcccctacc tggcactcga
    19981 gaaggacacg gcggacgcgc gcgacggcta ccccctggtg cgacgctccg gcgaggcgag
    20041 cgtcatcgcc gccggcactg ccgaacctac tgcctggtgg tccgcctcag gctcgctggg
    20101 cgccgcccag acaacgcgcc tggaggacgc gctgataaag gcgcgggaga cgttcacctt
    20161 cctcgccatt caccacccgc cgctgccagg catggcgccg cgccgaaagt cgcttcgcga
    20221 cgcgcacgcc ctggcagagc tcctcgaacg gcacgcgccc gacgtggtcc tgcacggcca
    20281 tctgcatgcc aatcaggaaa actccgacac gcccacacgc atattctgca cggcgccgcc
    20341 gtccagcgag cgaccgcggg cgcccgcttc ctaccgcgtg ttcgacgtcg tcgaagacgc
    20401 ggccgggtat cgcgtggtca tgcgcctgaa gtcgctgatc ggcgaggaga tggtggtcgc
    20461 agacgagcag tcgtggacct ccggccgatc actcgacccg ggtgagcgcg acgatgctgc
    20521 caagcagtag cgcggagtcc ggcagcatcg ccaggggtat gaccggaagc tcgtagagca
    20581 gtgcgagctg agcggacgtc tgctcgacca gataaaaacc gatgccgacc gtggcgccga
    20641 acaacaccct ggcgcccgct ggccgagccc gcaccgaacc gagaacgaac ggcaagccga
    20701 gcagacacat ggccatcagc gcaatgggcg agctgagcac ctgccacagc ctgaagcggt
    20761 agcgatgagc gttgagaccc gacgcctcca gatattcgac gtaccggtag aggtcgccca
    20821 gggtcagggt ttccatcggc gcgatgaggg aggccatctg gtccggagac aggaagctct
    20881 cccactcgta ggtgtcacgg ccggtgcgcc tgacctcttt gccgagaatt cggcgctctt
    20941 cgacaccgtg aagcagccac tggcccggcc cgatcacgtc cgcgtactcg gcctgcagca
    21001 cgcgctgcac gacgccgcga tcgccgagat cgtagatctc gacatcagcg ggaatcatgc
    21061 cgaaacggac gtcccccacc ctgatgaagt gagtgccgtc gcgggtccag aactcggtgg
    21121 tctcgccgcc acgcatcgtg ttgaccagcg acttcgcctg aagctccttg gcctgttcct
    21181 cgaaccgagg caccacgaac tgctgcgcca gcagcacgac gtaagacaac acgatggccg
    21241 ccagcgcaag cggataggcc atccgccatt gggatagacc aaccgctcgc agcgccgtca
    21301 gctcctgttc ctgagccagg ttgccgagcg ccatcacccc accgagcagc accgccaccg
    21361 ggagaagatc gatggcgatt cgcggaagcg tcagcagggc gacgcggatc gcgtccgctt
    21421 cctcgtactg ccccttgccg acgtcctcca gctcttcggc aagggtgacg aaaccgaaca
    21481 gccccagcag cagcaacaga acgggtaccg cgcccagcag caagacgcct acaaggtagc
    21541 ggtccacacg cttcacgcgc ttcgcccccg tcgtctgaac caccgggccc atggcacgta
    21601 ggccagcacg atgccgcctg caaaggtcgc cgggacccac cacaacgtcg ccgccgtgcc
    21661 ctgctcgacc caggtccttc cgatgcccag aacgttgaag tacacggcgt agatgaccac
    21721 ggcaaccgtg agccgggcaa agcgcccctg acggggccgg gaccgtccga gcaggacggc
    21781 cagcaacgcc agcagaatgg tcgaaacggg cgccgacagg cgccattggt actcggcctg
    21841 atccactagc gcgtcggatt ctctgaggac gaacgagttc tgggatttcg gcttgtagcc
    21901 cacgggctgc accgccgcca ccggaacgaa tatgcgcagc tcgccgaaga tgcccagaaa
    21961 gtcggcgccc tgcgcagttt tcctgtagac ctgagcatcg cgaaggtcaa cctggtggtt
    22021 cttgtcgctg acatactcgc tcacggtacc gctgggcgcg gagatgattt ccagatcatc
    22081 gccgtccagc gcgcgcacga acagcccctg aatctcgtcg tcttcggcct tcagctcctc
    22141 gatgaagacg gtccgggcat tgtcgtcgta caggtagaat cgcccgggtt tgatgcgctc
    22201 gagctccgag gtcgcttccg cttcgacctc cagcctgtag aggtgctcgt acgcccaggg
    22261 cctgcc
//
LOCUS       DBNHLM010000196         4709 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1583897, whole genome shotgun sequence.
ACCESSION   DBNHLM010000196 DBNHLM010000000
VERSION     DBNHLM010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1583897"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..709)
                     /gene="phoB"
                     /locus_tag="AC16QF_08970"
     CDS             complement(20..709)
                     /gene="phoB"
                     /locus_tag="AC16QF_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HIR6448731.1"
                     /translation="MSTNTILLVEDEPEIRDMLRFALTRSGFQVWDTESAEDAMARLD
                     GVLPGLIIVDWMLPGMSGVDLARRVRNDEVTGHLPIIMLTARGEETDKLKSFDSGVDD
                     YVTKPFSPKELVARVKALLRRSGSPQDGIIDTDGIQLDLNAHQLIIKGQPVKIGPTEF
                     RLLELLLSNPDRAFDRSQLLDRVWGRSVYVEERTVDVHVLRLRKVLKPYGLNRLIQTV
                     RGVGYRFSPGS"
     gene            992..2317
                     /locus_tag="AC16QF_08975"
     CDS             992..2317
                     /locus_tag="AC16QF_08975"
                     /inference="COORDINATES: protein motif:HMM:NF028240.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HIR6448732.1"
                     /translation="MIDLSNSFPTARRKPLLSVVSMVLAMLVVPPVQSSEVDLLIEKL
                     VAKGILNEGDAKEIRAEVARERQQAAAAPVEIQDLGTLPVAHAPAEAKEHWSDKISFK
                     GDLRVRYQGEELDNAPSIGPFDIDERNRWRIRWRAGVEADVSDRWEVGFGLASGGSDA
                     RSSNQTLRNAFSTGDARLDYAFARFKAGDGVDVVAGKFKNPIWKPKDLLWDSDIRTDG
                     VAVPMEFGVNDRVSAFVTPAYLVLTEDVIGGQDDAAMWLLQAGANFDLSDAVSLTVAP
                     TYYNFVNLKGSKGPITVDVPSNARGEDDRFVYDYDSIALGGKLDIDGTELIPRVSVFG
                     EWVKAFDPDEEDTGWLVGLALGDAKVSNFGDWQLKYNYRRLEADAWPEFLSDSDFFFG
                     ATNVKGSEFEFIWGLAKGVNVSVDYYTAAKFIGSDLEQDLLQLDLNVKW"
     gene            2441..3112
                     /locus_tag="AC16QF_08980"
     CDS             2441..3112
                     /locus_tag="AC16QF_08980"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6448733.1"
                     /translation="MGEKAQITSADGFTLGAYQATPAGQPKGAVVVIQEIFGVNQHIR
                     EVVDGYAADGYFAIAPQIFDRVERDIELGYEEADMGAGIEIAFQKLDHAKALEDVQAA
                     IEVASVQGKVGVVGYCFGGLLTWLAACELEGVSAASSYYGGGVAGEAQRQPRCPVIMH
                     FGELDAHIPMSDVEKVKAAQPDVPVFVYPADHGFNCDHRASYDAAAAATAKQRTLEFF
                     GRELA"
     gene            3381..4169
                     /locus_tag="AC16QF_08985"
     CDS             3381..4169
                     /locus_tag="AC16QF_08985"
                     /inference="COORDINATES: protein motif:HMM:NF023358.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 domain-containing protein"
                     /protein_id="HIR6448734.1"
                     /translation="MNTQRIHRSWGPVPLLLALLGLALCGNASAASLNDIFVVTDRLN
                     TQAKRSQAKIDALTEETRKLLSEYKTVLKEIEGLRVYNRQLEKQISSQEAEMAKLASS
                     IDQVTLIERQITPLMLRMIDGLEQFVELDLPFLQDERAGRIEALREMMDRADVAVSEK
                     FSQILRAYQIENEYGRTMEAYGDTINVAGVDLKADVLKVGRVTLVYQTPDGASTGRWD
                     PKAGQWVELGDEYTTPVRSGIRMARKQLSVDMLTMPVAAPEAAE"
     gene            4169..>4709
                     /locus_tag="AC16QF_08990"
     CDS             4169..>4709
                     /locus_tag="AC16QF_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448735.1"
                     /translation="MNHVKIKIGAGILALGLNGAALAEEAAPDVVVNQADTLEELLRN
                     VEERRVTESREHTQREQVFVREKNQQARMLADAQAERRREEQRSERLETAFEENEIRI
                     GDLQEQLDRRLGSLRELFGVLQQVAGDTRGLFEGSIISAEFPNRGEWLGQLAAKMGTA
                     SELATIDEMETLWFELQREM"
BASE COUNT          940 a         1343 c         1537 g          889 t
ORIGIN      
        1 ggagattttc atgggttggt cacgaacccg gcgagaaacg atagcccaca ccgcgaacgg
       61 tctggatcag tcggttcagg ccatacggct tgagtacctt gcgcagcctg agcacgtgca
      121 cgtccacggt tcgttcctcc acgtacacgc tccgccccca caccctgtcg agcaactggg
      181 agcgatcgaa ggctcgatcc gggttgctga gcaaaagctc cagcagccgg aactcggtgg
      241 ggccgatttt caccggttgt cccttgatga tcagctgatg ggcgttcaga tccagctgaa
      301 tgccgtccgt gtcgatgatt ccatcctgcg gagagcctga tcgtctaagg agggctttaa
      361 cccgggcgac gagctctttc ggcgagaacg gcttggtaac gtagtcgtcg acgccggagt
      421 cgaagctttt gagcttgtcg gtttcctcgc ctcgtgcggt gagcatgatg atgggaagat
      481 gtccggtaac ctcgtcgttg cgaacgcgcc tagcgaggtc gaccccgctc atgccgggca
      541 gcatccagtc aacgatgatc agcccgggca gaacaccatc gagccgggcc atcgcgtcct
      601 ccgccgactc ggtgtcccag acctgaaagc cggagcgcgt gagggcgaat cgaagcatgt
      661 cccggatttc aggttcgtcc tcgaccagca aaatggtatt ggtgctcatc gctctcgcgt
      721 cttcgcggtc cccggctgtt cctgatgccg tcaggcgcca ttagatcggc gaattgtgac
      781 aaaaagatga acccagcgtc gccttttggg aatcgtggcg caaaagcggc cgaatgacgc
      841 gggatcggga cggtgctcgt tgtcattcgt gaccggcgct gcgccgtcac attgtcatca
      901 acccgtaaca ctgcgggtgc taatcttcgg ttcgccgctg ccagacggca tccgataacg
      961 gcagattgtt aacctacgat gaggttgtcc catgattgat ttgtccaact cgtttccaac
     1021 ggcgcgccgc aagcccctcc tgagcgtggt ctctatggtc ctcgccatgc tggtggtccc
     1081 gcctgttcag tccagcgagg tggatctgct catagagaag ctcgtggcca agggaattct
     1141 gaacgaaggc gacgcgaagg agatcagggc agaggttgcc cgggaaaggc agcaggccgc
     1201 agccgcgccg gttgagattc aggacctcgg aacgctgccg gtcgcgcatg cgcccgcaga
     1261 ggcgaaagag cactggtccg acaagatatc gttcaagggc gacctgcgcg tgcgctatca
     1321 gggcgaagag ctggataacg cccccagcat cggcccgttc gacatcgacg aacgcaaccg
     1381 ctggcggatt cgctggcgcg ccggtgtcga ggccgatgtc agcgatcgct gggaagtagg
     1441 cttcgggctg gcctcgggag gctcggacgc gaggtcgagc aaccagacgc tgcgcaatgc
     1501 gttctccacg ggcgacgccc ggctggacta cgcgtttgcc cgcttcaagg ccggcgacgg
     1561 cgtcgacgtg gtggcgggaa agttcaagaa tcccatctgg aagcccaagg atcttctgtg
     1621 ggattcggac attcgcaccg acggggttgc cgtgcccatg gagttcggcg tcaacgacag
     1681 ggtctctgcg ttcgttaccc ccgcttacct ggtcctgacc gaggacgtga tcggagggca
     1741 ggacgatgcg gccatgtggc tgcttcaggc gggcgcgaat ttcgatctct cggacgcggt
     1801 gtctctgacg gtcgcgccga cctattacaa cttcgtcaat ctcaagggca gcaagggtcc
     1861 catcaccgtg gacgtgccga gcaacgcgcg gggcgaggac gaccgctttg tttatgacta
     1921 cgactccatc gccctcggcg gcaagctgga catcgacggc accgagctga tcccgcgcgt
     1981 cagcgtcttc ggcgagtggg tcaaggcatt cgaccctgac gaggaagaca cgggctggct
     2041 ggtcgggctt gccttgggcg atgccaaggt gagcaatttc ggcgactggc agctgaagta
     2101 caactaccga cgcctcgagg cggacgcgtg gccggagttc ctgagtgaca gcgacttctt
     2161 cttcggggcg accaacgtca agggcagcga gttcgagttc atctgggggc tggcaaaggg
     2221 cgtcaacgtg tcggtggact actacacggc ggccaagttc atcggttccg acctggagca
     2281 ggatcttctg cagctcgatc tgaacgtgaa gtggtagcgc ctctggtgct tttccagcgg
     2341 ctgaggatga gctaaggttc gaccccgctc gacgcccgtc atgaggcgct gagcaaagcg
     2401 cccggattga ggcggcaccg acaccaggga ggaacggaca gtgggggaaa aagctcagat
     2461 cacgtcagca gacggattca ctctcggggc gtatcaagct acgcccgcag ggcagccaaa
     2521 aggcgccgtc gtggtcattc aggagatttt cggcgtaaat cagcacatcc gcgaagtggt
     2581 agacggttat gccgccgacg gttacttcgc catcgcgccg cagatattcg atcgagtgga
     2641 acgcgacatc gagctgggct acgaggaagc tgacatgggc gccggcatcg agatcgcgtt
     2701 ccagaagctc gatcatgcca aggcgctcga agatgtgcag gctgccatcg aggtggcctc
     2761 cgtgcagggc aaggtgggtg tagtcggata ctgcttcggc ggcctgctca cctggctggc
     2821 cgcctgcgaa ctcgaggggg tcagcgccgc gtcttcttat tacggtggcg gcgtggcggg
     2881 agaagcgcag cgtcagccgc gctgtccggt gatcatgcat ttcggcgagc tggacgcgca
     2941 catcccgatg agcgacgtgg agaaggtcaa ggccgctcag ccggacgtgc ccgtctttgt
     3001 ctatccggcc gatcacgggt tcaactgcga tcatcgggca agctatgacg cggccgcggc
     3061 agccactgcc aagcagcgca cgctggaatt tttcgggcgg gagctggcct gaccggcccg
     3121 tgcccatgcc gttccgggcg aaagtcccgt caagcttgac ttccgtgagg ggtttgatga
     3181 tccttcgatc attgaagagg ggcggggcac atcgggcgtg tcggagtgcg tgcaccccgc
     3241 gttttgcgga tgtacagcag gcgaacgtgc acttttttcg caacggctca aacgtgcaca
     3301 aaggtgtggt gcgcggcgtt ttcagcgccg gcatttcctg ctgttgcgct cattaagata
     3361 accattccgt aggaagtcgt atgaacacac aacgaatcca caggtcttgg ggaccagttc
     3421 cattgttgtt ggctttgctt ggcctggcgt tgtgtggcaa cgcgtcggcg gcatcgttga
     3481 acgacatttt cgtggtgacc gatcgcctca acactcaggc gaagcgatcc caggccaaaa
     3541 tcgacgcgct gaccgaggag actcgcaagc tgctcagtga gtacaaaacc gtgctcaagg
     3601 aaatcgaagg cctgagagtc tacaaccgac agctcgaaaa gcagatttcc agccaggagg
     3661 ccgagatggc caagctggca agctccatcg accaggtgac gctcatcgag cggcagatca
     3721 cgcctctgat gctgcgcatg atcgatgggc tagagcagtt cgtggagctc gatttgccgt
     3781 ttctgcagga cgagcgtgcg ggtcggatag aagctctgcg cgagatgatg gatcgggcgg
     3841 acgtggccgt atccgaaaag ttcagtcaga tacttcgcgc ctatcagatc gagaacgagt
     3901 acgggcgcac catggaagct tatggagaca cgatcaacgt agcgggtgtg gatctcaagg
     3961 cggacgttct caaggtgggg cgggtgacgc tcgtgtatca gacgcccgat ggggcctcca
     4021 ccggccggtg ggacccaaag gccggacagt gggtcgagtt gggagacgag tacaccaccc
     4081 cagtacgaag tggtatccgg atggcgcgca agcagctctc cgttgacatg ttgacgatgc
     4141 ccgtcgcggc tccggaggct gcagagtgat gaatcacgta aagatcaaga taggcgctgg
     4201 aattctggcg ctcgggctca atggcgctgc tcttgccgaa gaggccgcgc cggacgtggt
     4261 cgtaaaccag gccgatactc tcgaagagct cctgaggaac gtcgaggagc gcagggtcac
     4321 ggaatcccgt gagcatactc agcgcgagca ggtgttcgtt cgcgaaaaga accagcaggc
     4381 gcgcatgctc gccgatgctc aggcggaacg ccgacgtgag gagcagcgtt cggaaaggct
     4441 ggaaaccgcc ttcgaagaga acgagatacg tatcggcgac ctccaggaac agctcgaccg
     4501 gcgcttaggc tccctgcgcg agctttttgg cgtgctccag caggtagccg gcgatacccg
     4561 cgggttgttt gaaggctcga tcatctccgc cgagttcccc aaccgtggag aatggctcgg
     4621 ccagctcgcg gccaaaatgg gcaccgcgag cgagctggcg accatcgacg agatggaaac
     4681 cctctggttc gagctccagc gcgaaatga
//
LOCUS       DBNHLM010000197         3508 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1404517, whole genome shotgun sequence.
ACCESSION   DBNHLM010000197 DBNHLM010000000
VERSION     DBNHLM010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1404517"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="AC16QF_08995"
     CDS             complement(<1..802)
                     /locus_tag="AC16QF_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020289265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HIR6448736.1"
                     /translation="MTQQPTPLTVAISSRALFDLVDSNGVYETEGLEAYRRYQIGHED
                     DPLAPGEAYRFVKKLLNINKILNKRRVEVILLSRNSADTGLRIFNSIKACDLDITRAA
                     FCGGESPYRYIQAFGCSLFLSTHPDDVAHALEHGVAAATLLGTKGTTDPVEGEDLRLA
                     FDGDSVVFSDEAERVFQSEGLEAFSATEAAAADRPLEGGPFKAFLAALHDLQREFEGQ
                     HCPIRTALITARGAPAHERVIRTLRAWDVRLDESLFLGGMEKTEFLRAF"
     gene            complement(799..1005)
                     /locus_tag="AC16QF_09000"
     CDS             complement(799..1005)
                     /locus_tag="AC16QF_09000"
                     /inference="COORDINATES: protein motif:HMM:NF016231.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HIR6448737.1"
                     /translation="MEAMDNAELERWLEELRIEHRDLDEVIHHLLQSRHHDSMRIQRL
                     KKRKLKLKDMIARLESELIPDLDA"
     gene            1064..1552
                     /locus_tag="AC16QF_09005"
     CDS             1064..1552
                     /locus_tag="AC16QF_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR6448738.1"
                     /translation="MLIIFAAILATVAFIVLIRLLWLSAKPNRASTVITLAAAGLIIG
                     LGILAATGRLHWIAAVGAATVPFLRRAFRLIRYLPWVRQFYQQSRAGNQQQGSSASST
                     TGTMSRDQARQILGLGTHPTREEVVAAHRRLMQKVHPDRGGSTFLAQQLNEAKRVLLK
                     DA"
     gene            1666..2076
                     /locus_tag="AC16QF_09010"
     CDS             1666..2076
                     /locus_tag="AC16QF_09010"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR6448739.1"
                     /translation="MSSSRPFTERRALPRYRPTGLHVEVRRSGRLARTRAEALDFNRF
                     GIGILIDQPIAKDKKIFISLRHGQRVLENVVGVVHNCIPHNGRFRCGVQFRTRSETQF
                     DKDWVERVLLDLECVFNDARGEPNDEQQALAAAN"
     gene            complement(2059..2457)
                     /locus_tag="AC16QF_09015"
     CDS             complement(2059..2457)
                     /locus_tag="AC16QF_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448740.1"
                     /translation="MWLHFLNPQQREALLGLAHNVMVSDGLLDPNEEGMLDEFKREME
                     LYPGTELEYLELDGIGETFASRRSRMIALLNLLRLSYADGAFEIEEECLLKEVSRAFE
                     VSDADFMLLDNWVKRLVALEEEAKALVGSG"
     gene            complement(2467..2634)
                     /locus_tag="AC16QF_09020"
     CDS             complement(2467..2634)
                     /locus_tag="AC16QF_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448741.1"
                     /translation="MSLISSVLRPVFYTMEDFMEQTLVMKAVIAWGWLSVIVMVVSFV
                     ATLPELLGAGG"
     gene            complement(2662..>3508)
                     /locus_tag="AC16QF_09025"
     CDS             complement(2662..>3508)
                     /locus_tag="AC16QF_09025"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR6448742.1"
                     /translation="LRSRALPDLLEEADRLAEVGVEELLVISQDTSAWGTDLRRSELR
                     GLKPGEALELLCRELAARFPWVRLHYLYPYPHVDQLLDLMAEGCLLPYLDVPLQHADP
                     AILKAMRRPAAAQNTLDRLARWRDRCPEVSVRSTFIVGFPGEGQREFETLLEFLEAAE
                     LDRVGCFAYSNVAGAGANNLPDQVPEPVKQERIERLMTLQARISREKLARKVGQELEV
                     IVDAVEDSLALGRTRFDAPDVDGVVRVHGGRSLVPGERTWVRITGSDTHDLDADYVGN
                     PLKFA"
BASE COUNT          657 a         1148 c         1071 g          632 t
ORIGIN      
        1 caaaggcccg taaaaactcg gtcttctcca tcccgcccag gaagagggac tcgtcgagac
       61 gcacgtccca ggcccgcagg gtgcggatga cccgttcgtg ggccggcgcg ccgcgcgccg
      121 tgatcagtgc ggtccggatg gggcagtgct gtccctcgaa ctcgcgctgg aggtcgtgca
      181 gggcggcaag gaacgccttg aagggcccgc cctccaacgg ccggtcggcg gccgccgcct
      241 cggtggcgct gaaggcttcg agcccctcgc tctgaaacac ccgctcggcc tcgtccgaga
      301 agaccaccga atcgccgtcg aaggcgaggc gcagatcctc gccctccacc ggatccgtcg
      361 tgcccttggt gccgagcagc gtagcggcgg cgacgccgtg ctccagggcg tgagccacgt
      421 cgtcaggatg ggtggagaga aagaggctgc acccgaaggc ctgaatgtac cggtacgggc
      481 tctcgccgcc gcagaacgca gcccgcgtga tgtcgaggtc gcaggctttg atggagttga
      541 atatgcgcag cccggtatcc gcggagttgc gcgacagtaa tatcacttca actctgcgtt
      601 tattcaatat cttattgata tttaataatt tttttacgaa ccgataggct tctccaggcg
      661 ccagagggtc gtcctcgtga ccgatctggt aacggcggta cgcctcgagc ccctccgttt
      721 cgtagacgcc gttgctgtcc accagatcga aaagcgccct ggagctgatg gcgacggtga
      781 gcggtgtcgg ttgctgggtc atgcgtccag gtcgggaatg agctcgcttt ccagccgagc
      841 gatcatgtct ttgagcttga gcttgcgctt cttcagacgc tgaatgcgca tgctgtcgtg
      901 atgccgcgac tgaagaaggt gatggatgac ctcgtccagg tctcgatgct cgattcgcag
      961 ctcttcaagc cagcgttcga gctcggcatt gtccatcgct tccatggcat cattgtgcag
     1021 cctaacccgg caatgataac aggaagccaa cgggcaacag cggatgctca tcatatttgc
     1081 cgccatcctc gccacggtcg cgttcatcgt gctgatccgg ctgctgtggc tgagcgccaa
     1141 gccgaaccgt gcgagcacgg tcatcaccct ggcggccgcg ggattgatca tcgggctcgg
     1201 catactggcg gcgacgggca ggctgcactg gattgccgcc gtgggggccg ccaccgtccc
     1261 cttcctgcgc cgcgcgttcc ggctcatccg ctacctgccc tgggtgcgcc agttctacca
     1321 gcagagccgg gccggcaacc agcagcaggg ctctagtgcc agctcgacca cggggaccat
     1381 gtcccgtgac caggctcggc aaatactcgg tctcggcacc catcccaccc gggaggaggt
     1441 ggtcgccgcc caccgacgtc tcatgcagaa ggttcatccg gaccgcggcg gatccacctt
     1501 tctggcccag cagttgaacg aggccaaacg ggtgctgctc aaggacgctt gactcaacgg
     1561 cccgaaacac gcctgcccgt tgacggtctc gggagccgac tcgcggcgaa aggggttgag
     1621 aagcccatca cgatttgtcg gaagggtttg acctaagctt ttatcatgtc ctccagccgc
     1681 ccattcacgg aacgtcgggc tctgccgcgc tacaggccga ccggcctgca tgtggaggtg
     1741 cgacgctcgg gacggctggc ccgcacccgg gcggaggcgt tggacttcaa ccggtttggg
     1801 atcgggattc tcatcgatca gcccatcgcc aaggacaaga agatcttcat ctcgctcagg
     1861 cacggccagc gggttctcga gaacgtcgtc ggcgtggtgc acaactgcat cccccacaat
     1921 ggccggtttc gctgcggcgt tcagttcaga acgcgctctg agacgcaatt cgacaaggat
     1981 tgggtcgaga gggtgctgct ggatctggag tgcgtcttca acgacgcacg aggtgagccg
     2041 aacgacgaac agcaggcgct agccgctgcc aactaacgcc ttcgcctcct cctccagcgc
     2101 caccaggcgc ttcacccagt tgtccagcag catgaagtcg gcgtcgctga cttcaaaggc
     2161 gcggctcact tccttgagca gacactcttc ctcgatctcg aacgcgccgt cggcgtagct
     2221 caaacgcagc aggtttagca gcgcgatcat ccgtgagcgt ctggaagcga aggtctcgcc
     2281 gatgccgtcg agctcgaggt attcgagctc cgtgcccgga tagagctcca tctcacgctt
     2341 gaactcgtcg agcatcccct cttcgttcgg atccaacagg ccatcggaaa ccatgacgtt
     2401 gtgagcgagc cccagaaggg cttcgcgctg ctggggattc aggaaatgaa gccacacggc
     2461 ttcgcgctag cccccggcgc cgagcagttc gggcagagtt gctacaaagc tcaccaccat
     2521 cacgatcacg gacagccaac cccaggcgat gacggccttc atgaccaggg tctgctccat
     2581 gaagtcctcc atggtgtaga acacgggtcg caggacggac gaaatcaggc tcatactcgg
     2641 ctccagcaaa ctgcgcgcag tttaagcgaa cttgagcgga ttgcccacgt agtcggcatc
     2701 gaggtcgtgg gtatcgctac cggtgatgcg cacccaggtt ctctcacccg gtaccagcga
     2761 tcgtcctcca tggacacgaa ccacgccatc cacgtccggc gcatcgaatc gcgttcgccc
     2821 gagcgcaagt gagtcctcca ccgcatccac gatcacctcc agctcctgcc ccaccttgcg
     2881 cgcaagcttc tcccggctga tccgggcctg cagcgtcatg agcctctcga tccgctcctg
     2941 cttgaccggt tccggaacct gatcaggcag gttgttggct ccggcacccg ccacgttcga
     3001 gtaggcgaag caccccaccc gatccagctc ggctgcttcg aggaactcga gcagagtctc
     3061 gaactcgcgc tgcccctctc cgggaaagcc gacgatgaac gtgctgcgga cgctgacctc
     3121 aggacagcga tcccgccagc gagccagccg atcgagagta ttctgcgcgg ctgccgggcg
     3181 gcgcatggcc ttgaggatgg ccggatccgc gtgctgcagc ggcacgtcca gataaggaag
     3241 cagacacccc tccgccatga ggtcgaggag ctgatccacg tgggggtaag ggtagagata
     3301 gtgcagacga acccagggga aacgggctgc gagctcgcga cacagcagct ccagggcctc
     3361 gccgggcttg agcccacgca gctcgctccg gcgcaggtcg gttccccacg cgctcgtgtc
     3421 ctgagagatg accagcagtt cctcgacacc aacctcagcg agacgatccg cctcctccag
     3481 caggtccggc agcgctcgag agcgcagc
//
LOCUS       DBNHLM010000198         5930 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_745519, whole genome shotgun sequence.
ACCESSION   DBNHLM010000198 DBNHLM010000000
VERSION     DBNHLM010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_745519"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="AC16QF_09030"
     CDS             <1..1084
                     /locus_tag="AC16QF_09030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6448743.1"
                     /translation="MIGGVLVWSATTISCGLSRTYTQILVSRLGVGAGEASLAPAAWS
                     VLSDYFRPERLSRPISVYLMGPYLGAGIAMIAGAQVLDWTRDVDFVEVPLLGQLAPWQ
                     LTFIAVGLPGVLIAALVGTVREPARHGRKDAELIAPPWRDVLGYLWRHRGIYTSLHLG
                     VPCIVVMLYGLQAWTPTVLVRVYEWDLAQAGRVYGFIALFAGSAGVLSGPTVARVLER
                     RGYPDYPLRLAVIAAGCATLSMVALPWQSSAEGALVCIALASFFVTLPLALMTSTMQF
                     VTPNEMRGVVAGMYVVTTNVLGLGLGPTLVAASTDFIFADTSALAYSMALVSVVMGPM
                     AVVLLSVGMKPYITRAAELGAQGGKP"
     gene            complement(1099..2556)
                     /locus_tag="AC16QF_09035"
     CDS             complement(1099..2556)
                     /locus_tag="AC16QF_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HIR6448744.1"
                     /translation="MGLDVEIPGLTQQFIGGVWRDSSPDTVVDVISPSTEEVLTRVAA
                     PTVADADRAVAEARAAFDMGPWALMSVEERVEVCARLCDALEARLDQLNRAWAFESGA
                     TIAHGEMINSGAGVAIWRNALEAAASLPWQEDRGDTLIVREPIGTVLGILTFNGPIVL
                     MGMKIIPALLAGCTVIVKHAPESPLTSRLIAEAIREADFPEGVLSVLAADTPVTQHLV
                     GHPDVDMVALTGGTAIGVDVVKRTADRLARTALELGGKSPAIIAEDVDLEDVMATLVD
                     GASGFMGQVCVSLSRILAPRSRYGEVVDAFAERYAALRVGDPFDPATDRGPLAVERAR
                     ERTERYVAGAHQEGARIVTGGRRPPHLDRGWYYEPTLLADVHNGMTVAREEIFGPVTA
                     VIPYEGIDEAVQIANDSPFGLAASVYTRDQDLAMQVARQLRSGSVAVNAAGVSLTQPF
                     GGYKQSGWGRECGPEGILEFTQIKQIVLGASYLDG"
     gene            2688..3599
                     /locus_tag="AC16QF_09040"
     CDS             2688..3599
                     /locus_tag="AC16QF_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448745.1"
                     /translation="MSRGAPIDLARGAVIRLVLGIISVAIAVACDRTMGTARDSDAAV
                     PSGSFSLPATEAVAETITEPARLVELFAALEETGLRVHHVDHEGEAAMLLVEVDEQHF
                     EPVLVGADGRSRTGVTAVEALTDFGLDVVIGSSFVSELHSLTPVGLLQIEGTVISELQ
                     AHGYTRILGIRPAGLGVVPIRAYHRGMFDSAVQAGPGVVEEGRLDISERDLTRPKYYR
                     TFVGLCNGKALLGASQVPMNLHTVGKRLLEYLGETGRTCDEVVNLAGDREVVLALRSA
                     DKRRVAFFGHPLTAKAGLLGFRRRPKD"
     gene            complement(3602..4846)
                     /locus_tag="AC16QF_09045"
     CDS             complement(3602..4846)
                     /locus_tag="AC16QF_09045"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HIR6448746.1"
                     /translation="MSLTDQSGLRVRPICLVVLLAFLAACQESDRTQIAVRDLPVPAG
                     ASSQLPHLSKSEDGTVVMSWVEQRHATATLRHARLSDAGWTLPVTVASGDRWFVNWAD
                     FPSVVPIRGRIWAAHWLTKSRGGYYAYDVAMATSRDAGATWSSPSTPHQDGTATEHGF
                     VTLFPWNGDVGVVWLDGRRTAEPPSGSAASKGMTLRGARIGPDGDARDGELIDDLVCD
                     CCQTDAAMGPDGPFVVYRNRTTDEIRDIRLARFVDDAWEADISVGEDGWEIAGCPVNG
                     PAIDVVGNSVAVAWYTEAQGRARVRLSRSRDGGAGFGPPIDVSADRPIGRVDVVQSPD
                     GETFVSWLRRGDDDSAELRLSRLAPDGSVAMETVVARTSAARPSGFPQMISDAHSLIF
                     AWTEPGEDDSRIRSARVELTER"
     gene            complement(4843..5304)
                     /locus_tag="AC16QF_09050"
     CDS             complement(4843..5304)
                     /locus_tag="AC16QF_09050"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR6448747.1"
                     /translation="MLFALTLAAVTSLLSPADDLQHTAARAERLGLPILLYVSRSDCS
                     FCRRFENDVLFPLIRSGEIDGKILIRELEWDRSAPIADFTGTRISAATLAENYDAKLT
                     PTLLFLDHEGKELISRMTGYHESDYASYYLEAAVDLAFQRMSATIQGETGK"
     gene            5415..>5930
                     /locus_tag="AC16QF_09055"
     CDS             5415..>5930
                     /locus_tag="AC16QF_09055"
                     /inference="COORDINATES: protein motif:HMM:NF016064.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HIR6448748.1"
                     /translation="MSKDYIISWKVAGVALGLLLTLATALVQPLGVSTQFVVTDAMVA
                     HKVAPQFAESNEYLAKYGEKTDWGVGYGWMLFLGMLVGGAVTHLVLRNRQPEADKGSM
                     PAMWVARFGDSHAKRYAAAFFGGVLLLFGARLAGGCTSGHMISGISQLAVGSFIFGVM
                     TFGSAILMAKFL"
BASE COUNT          931 a         1937 c         2000 g         1062 t
ORIGIN      
        1 catgatcggc ggcgtgctgg tctggagcgc gaccaccatc tcctgcgggc tgtcgcgcac
       61 ctacacccag atactcgtct cgcgcctggg cgtgggggct ggcgaggctt cgctggcgcc
      121 cgcggcatgg tccgtgctgt cggactactt tcgcccggag cgtctttcgc gccccatcag
      181 cgtctacctg atgggtccgt acctcggcgc cggtattgcc atgatcgcag gcgcacaggt
      241 gctcgactgg acccgcgacg tcgacttcgt ggaggtgcct ttgctgggcc agctggcgcc
      301 atggcagctg accttcatcg cggtagggct gcccggcgtg ctcatcgcgg cgctggtcgg
      361 cacggtgcgt gaaccagccc gtcatggtcg caaggacgcc gagctcatcg ccccgccctg
      421 gcgggacgtg cttggctacc tgtggcgaca cagaggcatc tacacctccc tgcatctcgg
      481 ggtgccctgc atcgtggtga tgctttacgg gctgcaggcg tggacgccca cggtgcttgt
      541 gagggtctac gagtgggatc tggctcaggc ggggcgggtc tacggcttca tcgcgctctt
      601 tgccggttcc gcgggggtgc tgtcggggcc caccgtggca cgtgttctgg aacggcgcgg
      661 ataccccgac tacccgctgc ggcttgccgt gatcgctgcc ggctgcgcga cgctgagcat
      721 ggtcgcgctg ccctggcagt caagcgcgga gggtgcgctg gtctgcatcg cgcttgcgag
      781 cttcttcgtc actctgcctc tggcgctcat gacgtccact atgcagttcg tgacgcccaa
      841 cgagatgcgc ggtgtcgtcg cggggatgta cgttgttacc accaacgtgc tcggtctggg
      901 gctcgggccc acgctcgtcg ccgcttccac cgatttcatc tttgcagaca cctcggcgct
      961 ggcctattcc atggcgctgg tctctgtcgt catggggcct atggcggtgg tgctgctgag
     1021 cgtcggcatg aagccctaca tcacccgtgc ggcggagctc ggcgcgcagg gagggaagcc
     1081 gtaggccggc ctgccagatc agccgtccag atagctggcg ccgaggacga tctgcttgat
     1141 ctgggtgaac tccagaattc cttccggtcc acactcgcgg ccccatcccg actgcttgta
     1201 cccgccgaat ggctgggtca gcgagacgcc ggcggcgttc acggccacgc tgccgctgcg
     1261 cagctggcgc gccacctgca ttgccagatc ctggtcgcgg gtatagaccg acgccgcgag
     1321 cccgaaaggt gagtcgttgg cgatctgcac cgcttcgtcg atgccctcgt agggaatgac
     1381 cgcggttacc gggccgaaga tctcttccct ggccaccgtc atgccgttgt ggacgtcggc
     1441 cagcagggtc ggctcgtagt accagccgcg gtccaggtgg gggggccgcc gaccgccggt
     1501 gacgatccgg gcgccttcct gatgcgcgcc ggcgacgtag cgttcggtgc gttcccgggc
     1561 ccgctccacg gccagtgggc cgcggtctgt ggcgggatcg aaggggtcgc ccacgcgcaa
     1621 ggcagcgtag cgctcggcaa aggcatcgac cacctcgccg taacgactgc gcggcgcgag
     1681 tatgcgcgag aggctcacgc aaacctggcc catgaagccg gacgcgccgt cgacgagggt
     1741 cgccatgacg tcctccaggt cgacgtcctc ggcgatgatg gccggcgact tgccaccgag
     1801 ctccagcgcg gtgcgggcca gcctgtcggc ggtgcgtttg accacatcta cgccgatggc
     1861 cgtgccgccg gtcagggcca ccatgtcgac atcgggatgg cccaccagat gctgagtgac
     1921 cggcgtgtcg gcggccagga cgctgagcac gccctccgga aagtccgctt cacggatggc
     1981 ctcggcgatg agcctggaag tcaggggcga ctcgggagcg tgtttcacga tcaccgtgca
     2041 cccggccagc agggcgggaa tgatcttcat gcccatcagc acgatgggcc cgttgaacgt
     2101 cagaatcccg agaaccgtgc cgatgggctc ccggacgatg agcgtatccc cccggtcttc
     2161 ctgccacgga agtgacgcgg ccgcttccag cgcgttgcgc cagatggcca cgccggcgcc
     2221 ggagttgatc atctcgccgt gagcgatagt ggcgcccgac tcgaatgccc aggcgcgatt
     2281 cagctgatcc agacgggcct cgagggcgtc gcaaagccgt gcacagacct cgacgcgttc
     2341 ctcaactgac atcagggccc acggtcccat gtcgaacgcc gctcgcgctt cggcgacagc
     2401 ccggtcggcg tctgccaccg tcggcgcagc cacccgtgtg agcacctcct cggttgaagg
     2461 agatatgacg tccacgaccg tgtccggtga agaatcgcgc cagacgccgc cgatgaactg
     2521 ctgtgtcagc cctggaattt ccacgtctag ccccatggcc cgttccctca aatggaaaaa
     2581 gttagtttgc gacaaaggcg cgtcgatcgc cacagcgggc cgcgcggcgt gcccgggtga
     2641 tgccataatg gcggcctgtc ggcatgcaat acggggaatc agatcaggtg agcaggggcg
     2701 ccccaatcga cttggcccgc ggggccgtga tccgcctcgt cctcggcatc atctcggtgg
     2761 cgattgccgt tgcctgtgat cggacgatgg gcacggcgcg ggacagcgac gctgcggtgc
     2821 ccagcggaag cttttcgctg cccgcgacgg aagcggtggc ggagaccatc accgaaccgg
     2881 cacgtctcgt cgagctgttc gcggcgctcg aggaaaccgg gttgcgggtc caccacgtcg
     2941 atcatgaggg cgaagccgcc atgctgcttg tcgaggtgga cgagcagcac ttcgagcccg
     3001 ttctggttgg cgccgacggg cgcagccgaa cgggtgtaac cgccgtggag gcgctcaccg
     3061 atttcgggct ggacgtcgtc atcggtagca gcttcgtctc cgagctgcac tccctcaccc
     3121 cggtgggcct gctccagatc gaaggcaccg tgatcagcga gctgcaggcc catggttaca
     3181 cccgaattct gggcatccgg ccggccggtc tcggtgtggt tccgatccgg gcctatcacc
     3241 gcggcatgtt cgactccgcc gtgcaagccg gtcctggggt cgtggaggag ggcaggctcg
     3301 acatctccga gcgggacctg accagaccca agtactaccg gaccttcgtg ggcctttgca
     3361 acggtaaggc cctgctgggc gccagccagg tccccatgaa cctccatacc gttggcaaac
     3421 ggctgctcga atacctcggg gaaacgggac ggacgtgtga cgaggtggtc aacctggccg
     3481 gggatcgcga ggtggttctg gcgctgcggt cggccgataa gcgtcgggtc gcctttttcg
     3541 gtcatccgct gacggccaag gccggattgc tcggttttcg ccgccgcccg aaggactgaa
     3601 atcagcgctc ggtgagctcg acccgggcgg agcgaatgcg cgaatcatcc tcgcccggtt
     3661 cggtccacgc gaaaatcagg ctgtgggcgt cggagatcat ctgtgggaac cccgagggcc
     3721 gggccgccga cgttcgggcg acgaccgtct ccatggcgac ggagccgtca ggcgcgaggc
     3781 ggctcaaacg cagctcggcg gaatcgtcgt ccccgcgacg cagccagctg acaaacgttt
     3841 cgccgtccgg ggactgaacc acgtccacgc gcccgatggg gcggtcggcg ctcacgtcga
     3901 ttggcgggcc gaagcccgcg ccgccgtccc tcgaccggct cagccggacg cgcgcccggc
     3961 cctgggcctc cgtgtaccag gcgacggcca cagagttgcc gacaacatcg atggcggggc
     4021 cgttgaccgg gcagccggcg atctcccacc cgtcctcgcc aacggagata tccgcctccc
     4081 atgcgtcatc gacaaagcgg gccagccgaa tgtcccggat ctcgtccgtc gtccggttcc
     4141 ggtagaccac gaaaggacca tccggaccca tggccgcgtc cgtctggcag caatcgcaga
     4201 cgaggtcgtc gatcagctcg ccgtcccggg cgtcgccatc gggaccgatg cgagcgccgc
     4261 gcagggtcat ccccttcgat gctgcgctgc ccgacggggg ttcggccgtc cggcggccgt
     4321 cgagccagac cacgcccacg tcgccattcc acgggaacag ggtcacgaag ccgtgctcgg
     4381 tcgcggtccc gtcctgatgg ggcgtgctgg gcgacgacca ggtggcgccc gcgtcacgcg
     4441 acgtcgccat cgcgacgtca taggcgtagt acccgccccg gctcttcgtc agccaatggg
     4501 cggcccagat cctgccacgg atgggaacga cggatgggaa gtcggcccag ttgacgaacc
     4561 atcggtcacc gctggccacg gtgaccggca gggtccagcc cgcatcggac agccgcgcat
     4621 ggcgcagcgt ggctgtggcg tgtcgttgct cgacccagct catcaccacg gtgccgtcct
     4681 cgcttttcga aaggtggggc agctggctgc tcgcccccgc cggcaccggg agatcccgta
     4741 cggcgatctg cgtccggtcg gattcctggc aggctgcgag gaaggccagc aggacgacga
     4801 ggcagatcgg gcgaactcga aggccggatt ggtcagtcag gctcattttc cggtttcccc
     4861 ctgtatggtc gcgctcatcc gctggaaagc gaggtccacg gcggcctcca gatagtagga
     4921 ggcgtagtcc gactcatggt agccggtcat tcgtgagatg agctctttac cctcatggtc
     4981 gagaaacagg agggtcgggg tgagctttgc atcgtagttt tcagccaacg tcgcggcaga
     5041 gatccgtgtg ccggtgaagt cggcgattgg cgcgctgcgg tcccactcca gctcccggat
     5101 gagaattttc ccatcgatct cgcccgagcg gatcagggga aacagcacgt cgttctcgaa
     5161 gcgccggcag aaactgcagt cgcttcggga cacgtacagg agaatcggca ggccgagccg
     5221 ttcggctcgg gcagccgtat gctggaggtc gtcggcggga gacaggagac tcgtcacggc
     5281 cgccagggtg agtgcgaaaa gcaaacgtct actcctgagt ccgccccgga agagggggcc
     5341 ttttatcagt tttcattaat atacgaaaat gataatgtac gttcctgtca tcgttcaccg
     5401 tggaggttcc tccgttgagc aaggattaca tcatttcctg gaaagtcgcc ggggtcgcgc
     5461 tgggtctgct gctgaccctt gccaccgccc tcgtccagcc gctcggtgtt tctacccagt
     5521 tcgtcgtgac ggacgccatg gtagcgcaca aagtcgcgcc gcaattcgcc gaatccaacg
     5581 agtacctcgc gaagtacggc gagaagacgg attggggcgt cggctacggc tggatgctgt
     5641 tcctgggcat gctggttggg ggcgcggtca ctcatctcgt tctccgcaat cgccagccgg
     5701 aagcggataa aggctcgatg ccggccatgt gggtcgcccg gttcggtgat tcccacgcga
     5761 agcgctacgc ggcggccttc ttcggcggcg tgctgctgtt gttcggggcg cggcttgcag
     5821 gagggtgcac cagcggccac atgatctccg gcatctcgca gctggcggtg gggtcgttca
     5881 tcttcggggt catgaccttc ggttccgcca ttctgatggc caagtttctc
//
LOCUS       DBNHLM010000199        15841 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_208055, whole genome shotgun sequence.
ACCESSION   DBNHLM010000199 DBNHLM010000000
VERSION     DBNHLM010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 15841)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15841)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15841
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_208055"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="AC16QF_09060"
     CDS             <1..226
                     /locus_tag="AC16QF_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6448749.1"
                     /translation="LLCDEPTGALDVTTGIVVLEAIERVNRELGTTTAVITHNTVIGA
                     MADRVMHMADGLIARVDENPDRQPPDELSW"
     gene            250..2595
                     /locus_tag="AC16QF_09065"
     CDS             250..2595
                     /locus_tag="AC16QF_09065"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6448750.1"
                     /translation="MLRDLVHMKGQVIAVGLVIASGVAVLVMALSTLEALRETTDAYY
                     ERYRLADVFAGVKRAPEPLSDRIREIEGVQTVETRIVKFATLDVLHFPEPVMGQFVSL
                     PESGPPLLNQLALRAGRHVEPGQHDEVVVNEPFAEAHGLTPGDSLVAVLNGKRRLLRV
                     TGIALSPEFIYSLGPGALMPDDERFGVIWMNRKALAAAFDLKDAFNNISLQLTRGTRP
                     EGVITQLDRLLEQYGGIGAVARANQMSNWFVMNEIDQLATISRILPSIFLLVSAFLTN
                     MVLTRLIDTERAQIGLMKAFGYTSFEVGFHYTKLVLGMAVVGIVVGLFVGGWFGRLNT
                     QMYAEVFRFPLLLYRPSPSAFAIASALSLLAALAGAMSSVRRATRLPPAQAMQPPAPP
                     TFRRGRLAHSRFGRWLDQPTRIVLRNIARWPGRAALTTTGIAASVALLVLALQWNDSL
                     NYLAQSYFFQAQRQHVMIGLADTRSATVMHDIEQLPGVLAAEPMRIVSADLSRGAVTH
                     RGALTGVADGAQLQPIYDDARGVEIPTPPEGLVLGITLAEKLGVGVGDRIWVNVLEGR
                     RPNVQLPVVEVVETYIAMPAYIHIDALNRLLKERPSVEYANLRIDRLEESRLYAELKK
                     LPVVSAVMLRQAAIDAFYDNVVEHLMVFITMFSSLACILGFGVAYNSVRIALSERGRE
                     LATLRVLGFTRGETSYILLGEVALLIVAALPLGCLLGRLLSQLIAAAFNTELFRVPLT
                     IDPSTYGIAVLIALAATTLSAALVRRRVDRLNMIEVLKTRE"
     gene            2598..3806
                     /locus_tag="AC16QF_09070"
     CDS             2598..3806
                     /locus_tag="AC16QF_09070"
                     /inference="COORDINATES: protein motif:HMM:NF052847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6448751.1"
                     /translation="MNANAKRILVWSVVAIAVAVGLTIAFMPRPVTVDLITVIPAPME
                     VTADEEGETRVHDVFVLSAPVAGRLRRIDAHVGDPVLADETVLARIEPADPTFLDPRT
                     EAQARAARQAAVSALTLAKARVEEARAEFEYADAEFHRARELIAKGTISVRDAEAAER
                     AYKARRAALSTAQAAVQMRTYELQQAEALLISPAQSRTRTDECDCVDVTAPVSGQVLQ
                     IVNPSERIVTAGEALVEIGDPGDLEIVVDFLSADAVQIEPGQRVIIERWGGESALVGH
                     VRRIEPFGFRKTSALGIEEQRVNVIVDFTSPRNEWSRLGHGYQVDVRVVLWEQDAVLA
                     IPLTALFRDDQRWAVFVDDDGRAALREVVVGHRNGMVAEIVSGLARGDRVVLHPSNRV
                     IDGVRITGRG"
     gene            complement(3841..5391)
                     /locus_tag="AC16QF_09075"
     CDS             complement(3841..5391)
                     /locus_tag="AC16QF_09075"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: protein motif:HMM:NF004829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HIR6448752.1"
                     /translation="MSSYDVAIVGMGPVGAAAAILFADAGLKVIAFERDAHVYPLPRA
                     VALDGEVVRGFQRVGRAGELAALLQPVRPGERAGFANSRREWLFGQPYATFGVNGWQP
                     ISMFDQPELEAHLRRLAMDHSGVTGCLEVQVETFEETGGQVAIRARQADGALSETSAR
                     FLVACDGAASGARRALEIGWRDLGYDHDWLVVDVVLKDGHTLGLDTLQVCDPDRLSTY
                     IATKDPFRRWEFKLNPGETREEMLEPERIRELIDPWTPRGTYEIRRSAVYQFHAATAE
                     TWRKGGVLLAGDAAHQTPPFLGQGLNAGMRDVINLAWKLPLVLSGAADEGLLDSYQAE
                     RGAHAADLVEWAVAIGKLMEHLAAVEAAQRVGRKPPEVPDGNRASGYGQGREAPPLRD
                     GVVMVDQVSEDGSTGYLFRQPAVRDADGEEYLLDDRLGAGFALVARSEADLAMSEASR
                     AIVDALGMSTTTLEGLEATRGRFDRLFEGSAAAVVRPDRYVFGHTSQSLSLDALLGEL
                     AARLGLRL"
     gene            complement(5388..6035)
                     /locus_tag="AC16QF_09080"
     CDS             complement(5388..6035)
                     /locus_tag="AC16QF_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6448753.1"
                     /translation="MPNQTHRGPVQERAQVTRDRLLDAAVRCFAAAGYEGSSTRQIEA
                     SAGVKRGLIAYHFGTKEALWKQAVTWWFGQAPHMLRVRVSGQGGSDPAAQLRALITGF
                     VRFSASFPEANRLMIREGMDDDWRLQWLAEHIVGPWYERLGAAFEEARSAGAVRNVPF
                     VHFYYILTGAGSLLFSMAPEAERLAGIDPYDDDVVRRHAEVIADLLIPGSGEHAH"
     gene            6034..6336
                     /locus_tag="AC16QF_09085"
     CDS             6034..6336
                     /locus_tag="AC16QF_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448754.1"
                     /translation="MDGKTLSFDELFYKSLVIRQVFIEEVAVSGMTDKLYARFRVPDL
                     NRQARSRSSRRRLGSQVFDYWKDPNGFTMEHFTDGDLFNASSGSEKWPVDVALGSH"
     gene            6416..7468
                     /locus_tag="AC16QF_09090"
     CDS             6416..7468
                     /locus_tag="AC16QF_09090"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6448755.1"
                     /translation="MLPGGLIFGTFLVLVLVLALVLVLVLVAGWYVWERRQRRTRAMP
                     GGLHEEITLPHRQAWELYHNNLSLCSKKTRACLSELSIDYESHHIDLIETGRYENVSR
                     EFLKVNPAGLVPVLVHEGHPVYESHAQIAFAAARSDDPHRLVPADPDQRRQMDDWVRK
                     TSLIGDDPVGAVHQTAGNAVPGLTLPLFATMLERIPLRRVLEGLLFHRLKRRAFILMI
                     IKLYGIDSLTSAKPLLRVVQASRDAMHRHLDDLEATLKDSGGPWIVGEHFSLADIGMM
                     AILERLREADWLDVLVSGRRPRVAAYWRALQTRPSYQSAIVACRGEAIERGTARLVAA
                     KQNNPVLRFALIETIT"
     gene            7516..8394
                     /locus_tag="AC16QF_09095"
     CDS             7516..8394
                     /locus_tag="AC16QF_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008094299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR6448756.1"
                     /translation="MKLVSFSTTENPKPRLGAVRDGGIADLSGSDGIPETMLGLLHAG
                     DAAMKAAHEAAEAAADTVPLDQVTLHPPVLTPGKILAIGLNYGDHIEESGMGKPKHQV
                     WFNKQHNCVNGPYGDIALPSVSEMLDYEAELVVVIGKRCKHVPRERAAEVIAGYCCGN
                     DVSVRDWQMRAQTMQIGKSFDTHGPTGPWLVTPDEVGDPHDLDIRAEVNGELRQNSNT
                     KHLIYDCYDAVAHLTQAFTLDPGDLLFMGTPAGVGAAMKPPQFLKAGDVVRVEIEKLG
                     YLENRVVPEAAECVIE"
     gene            complement(8396..8995)
                     /locus_tag="AC16QF_09100"
     CDS             complement(8396..8995)
                     /locus_tag="AC16QF_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6448757.1"
                     /translation="MAQAQTHDHEIVSIYPFTDEEVDQLMNNSVECVLMWATKDGWPV
                     GVTHAFVWHDGKIWLTFAAHRHRTVAIRRDNRVSVNVSSCGYPPGASEELPGGAITFK
                     GTGEFFDDEETKKWFYTALSQKLNPNSPEGEQFFYNLLDSPLRTVLAVTPVKKIMYNG
                     KLAGGHMAGTVKEEDLGPRLGVDKERMNAERAKRGLDPR"
     gene            9172..10395
                     /locus_tag="AC16QF_09105"
     CDS             9172..10395
                     /locus_tag="AC16QF_09105"
                     /EC_number="5.4.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR6448758.1"
                     /translation="MSNLRRSTQLYERAKAVIPGGIPGIRAPENFVPGAYPILLAGGR
                     GGHVTDVDGNDYVDLLLGYGPVILGHAEQAVDSAAHERAAHGFCLDLPEPVMIELAEQ
                     LVSMVPSAEQALLLKTGSDATSAAIRIARTHTGRSVVLRCGYHGWHDWCLQGDPGVPS
                     GTTDTVIAFPYNDLDRLAALLTAHSGDVATIMLTPVGHEFDRQIEQPEPGFLEGVRSL
                     ADQHGVVLAFDEVRTGFRVHPGGAQGLYGVIPDLTALGKAMSNGYAVTALVGRRDVMQ
                     SARSTFISSTYFPNGMEMAAALETLKLIAEHDVLTGIAATGAALAGGLSEIVARHGLP
                     VSLSPYDEMPFLHFDPDLASGQPERRDAFYGAMARSGVFAHPRHHGFLCWRHSADDVA
                     RVLEAVDAAAAALGS"
     gene            complement(10392..12638)
                     /locus_tag="AC16QF_09110"
     CDS             complement(10392..12638)
                     /locus_tag="AC16QF_09110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR6448759.1"
                     /translation="MTTDGAVTAGERSHLTFCRICESLCGLRVSTRDGVVTDVRPDGD
                     HVATGGFACPKGLRQHHLYGSPDRLLRPRKRVGGDWRDVSWNEALGDIGSELARIRDE
                     HGPDAIAMYVGTAAGFGVLHPVFAQGFMDGLGSRNMYSSATQDCANKFAVAERIYGFP
                     FTQPFPDLLNTKLLVIVGANPMVSKWSFLQVPNPRRHLNAIRERGGRVVVVDPRRTET
                     AKAAGEHVFIRPNSDLYFYLAFLHELIAAGGVREDRVDAHMNGFEEVRELVEPWTAER
                     AAQHTGVSAEVLRQLVVDYVAADGAALYSSTGVNMGAHGSLCFWIQEVINAVSGNLDA
                     RGGTLVGNGIFDFARFGKKYGLLVKDDRSRVGGFRKTNDAFPGGILADEILTPGPGQI
                     RALIVTGGNPLLTMADSERLKQAFGELELLVTLDILPTETGAAAHYMLPCTSPLERPD
                     LPFVFPLVLGMQARPYLQATNPVVDAPGEARDEATIYTELGTRAGAPLFGSPGFHRVM
                     RALKFCNRALTGRDEVPQRLLLNLILKLSRQPSFRRLLGHGRLRPPNVGGDFLGARVY
                     TDSARVELAPAELVSKLQATLASAREQGSASEFPFRLITKREVTTHNSWTHNYEGFLR
                     GDQGRNYVFMNPTDAAALGLKPDDLADVRSSAGAIRLPVRTSDDLLAGVVAVPHGWGH
                     QASGQRVAAGSLGVNVNILASSGAAHVDPFSGMSQLPGLPVDVRLAADDRLAGNWSGI
                     ARPPEGPD"
     gene            complement(12655..13179)
                     /locus_tag="AC16QF_09115"
     CDS             complement(12655..13179)
                     /locus_tag="AC16QF_09115"
                     /inference="COORDINATES: protein motif:HMM:NF025050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6448760.1"
                     /translation="MLDKPTDRSIFQNAWVVDDVEAACMKWVEQMGVGPFFVNEYDNA
                     FEQIIYRGEPAKLDMIVAIAQAGPVQVEIIQPKVDRCAYRDSVPAGTDAFHHMCVWTD
                     DIDADSEYFESLGYPTANRGAVMDIEFAYYDTRPLMGCMLEVVTRGPGIIERFAQIAK
                     AAENWDGKDPIRYD"
     gene            13362..14282
                     /locus_tag="AC16QF_09120"
     CDS             13362..14282
                     /locus_tag="AC16QF_09120"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HIR6448761.1"
                     /translation="MIDVVRTPEERFRGLPDFPYDPRYAAWSGLRVAHIDEGPADAPV
                     ALLLHGEPTWSYLYRKMIPPLLEAGYRCVAPDHVGFGRSDKPVEDDWYVIERHVERIT
                     FLIESLDLTDITLFCQDWGGPTGLRQAVDMPERFERLVIMNTWLHHEGYEYSPAVRFW
                     REAATNRAWLGWTGGDLPCGAIVAGTLARSGLDRAAVKRAYDAPFAEGGRSKAGARRF
                     PWCLPFGEPEAGNAADQARCFEALKSWAKPAHFIFGERDPIFTTGWARQWSALLPNAT
                     LDLIPDASHFVQEEAGEEIVAAFLERTRST"
     gene            14346..14732
                     /locus_tag="AC16QF_09125"
     CDS             14346..14732
                     /locus_tag="AC16QF_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6448762.1"
                     /translation="MIDINDRMAILDLCASYNYLFDTCAAKEWAATFTADGVFEGPAG
                     RAEGPEDLAEFCRNTNAQYPGAMHFTDNHLFEQNGDTVRHKCFLSMQVPSPEGTQTLL
                     LTYEDELRRVDGEWRFASRLCQPLSA"
     gene            complement(14744..>15841)
                     /locus_tag="AC16QF_09130"
     CDS             complement(14744..>15841)
                     /locus_tag="AC16QF_09130"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6448763.1"
                     /translation="RLPSSYDVNSPGGFPVLVYLHGWGGSFRSLPASAARHAKNNGYI
                     LVTPTGYGDGGRNSWNGYRSARLPVCALGDGYLASDCDAGPPELDSANGPSTCVDLDN
                     AGHDYCYDSCRTLYGAGAEPGNCPVWDGSQGDNTWGAEYNGDYTCHWTTCRDSVGQIA
                     ALLDEIEALYCVDRSMIWVTGCSNGGMMVYELAKDARTRTRFAGFMPQVGAPHPGFRQ
                     YDPASELVDRPRHLLGFWGETDRTVPGRANFPGTSPQVALDTSFNGWLYVPASDIGYE
                     WASMHDAAPLPVEEADAYARAYSNSLDCQVWQAQDEGETPGHASEVATCFFQGGHSCP
                     GFGGMEAMMWDFAGRHAKTDADPAACSTSSP"
BASE COUNT         2696 a         5400 c         5187 g         2558 t
ORIGIN      
        1 cctgctctgc gacgagccga ccggcgcgct ggacgtgacg accgggatcg tcgtcctcga
       61 ggccatcgag cgggtcaacc gcgagctggg cacgaccacg gcggtgatca cccacaatac
      121 cgtcatcggt gccatggccg accgggtcat gcacatggcg gacgggctga tcgcccgcgt
      181 cgacgagaat ccggaccgcc agccccccga cgagctgagc tggtaactgt gcgggcgctc
      241 gatacgaagg tgctccgcga cctcgtccac atgaaaggac aggtcatcgc cgtcggcctg
      301 gtcatcgcat ccggggtggc ggtgctggtg atggccctgt ccaccctcga ggcccttcgc
      361 gagaccaccg acgcctacta cgagcgttac cgcctggcgg acgtgttcgc cggggtcaaa
      421 cgggcgccgg agcctttgtc agacaggatt cgcgagatcg aaggcgtgca gacggtggag
      481 acccgcatcg tcaagttcgc aacgctggac gtcctccact tcccggaacc ggtgatgggc
      541 cagttcgttt ccttacccga aagcggcccg ccgctgctga accagctcgc gctgcgtgcc
      601 ggaaggcatg tcgagccagg ccagcatgac gaggtggtcg tcaacgagcc gttcgccgaa
      661 gctcatgggc tcacgcccgg cgactccctg gtggcggtgc tgaacggaaa gcgccgcttg
      721 ctccgcgtga cggggatcgc tctctctccc gagttcatct acagcctggg gcccggcgct
      781 ctgatgcccg acgacgagcg cttcggcgtg atctggatga accgcaaggc gctcgccgcc
      841 gccttcgacc tcaaggacgc gttcaacaac atctccctgc agctcacccg cggaaccagg
      901 ccggaaggcg tgatcacgca gctcgatcgg ctgctcgagc agtacggggg catcggcgcc
      961 gtcgcccgcg ccaaccagat gtcgaactgg ttcgtcatga acgagatcga ccagctggcc
     1021 acgatatcgc gcattcttcc gagcatattt ctgctggtct ccgccttcct gaccaacatg
     1081 gtgctcaccc ggctgatcga cacggaaaga gcccagatcg ggctgatgaa ggccttcgga
     1141 tacacgagtt tcgaggtggg ctttcactac acgaagctgg tgctgggcat ggccgtggtg
     1201 ggcatcgtcg tgggcctgtt cgtcggcggc tggttcggcc gtctcaacac gcagatgtac
     1261 gccgaagtat tccgcttccc cctgctcctc tatcggccga gcccgagcgc gttcgccatc
     1321 gcgtcggcat tgagcctgct ggcagcgctc gcaggtgcca tgagcagcgt ccgccgggcg
     1381 acccgtctgc ccccggccca ggccatgcag ccgccagcgc cacccacgtt tcgacgggga
     1441 cggctcgccc actcgcgctt cggccgctgg ctggaccagc caacccgcat cgttctgcgc
     1501 aacatcgccc gttggcccgg ccgcgccgcg ttgaccacca ccgggatcgc cgcttcggtc
     1561 gccctgctgg tcctcgcgct gcagtggaac gactccctga actacctggc gcagagctac
     1621 ttctttcagg cccagcgcca gcacgtgatg atcgggcttg cggatacccg gtccgccacg
     1681 gtgatgcacg acatcgagca gctcccgggg gtgctggcag cagagcccat gcgcatcgtc
     1741 agcgcagacc tatcccgagg agcggtaacc caccgcggcg cgctgacggg cgtcgccgac
     1801 ggcgcgcagc tgcagccgat ctacgacgac gcgcggggcg tggagattcc gacgccgccc
     1861 gaaggcctgg tgctgggcat caccctggcg gagaagctgg gcgtgggcgt gggcgaccgg
     1921 atctgggtaa acgtgctcga gggacggcgg cccaacgttc agctgcccgt cgtcgaggtg
     1981 gtggaaacct acatcgccat gccggcctac atccacatcg acgcgctcaa ccgcctgctc
     2041 aaggaacgcc cgagcgtgga atacgcgaac ctgcgcatcg accgcctcga agagtccagg
     2101 ctgtacgccg agctcaagaa gctgcccgtg gtctctgcgg tgatgctgcg ccaggcggcc
     2161 atcgacgcct tctacgacaa cgtggtggag catctgatgg tgttcatcac catgttttcc
     2221 agcctcgcct gcatactcgg gttcggtgtc gcctacaaca gcgtgcgtat cgctctgtcg
     2281 gaaagggggc gcgagctggc taccctgcgg gtgctcggct tcacccgagg ggagacgtcc
     2341 tacatcctcc tgggcgaggt ggcgctgctc atcgtggccg ccctgccgct gggctgcctg
     2401 ctcggccgac tcctctccca gctgatcgcc gccgccttca ataccgagct gttccgcgtg
     2461 cccctgacca tcgatccgtc cacgtacggc atagccgtgc tcatcgcgct ggcggccacg
     2521 acgctgtccg cggccctcgt tcgccgacgc gtcgaccgcc tgaacatgat agaagtgctc
     2581 aagacccggg agtagaaatg aacgccaacg ccaagcggat actggtctgg tccgtcgtcg
     2641 ccatcgccgt ggccgtcggc ctgacgatcg cattcatgcc cagaccggtg accgtggacc
     2701 tgatcacggt cattccggcg cccatggagg tcaccgccga cgaggaaggt gagacgcgcg
     2761 ttcacgacgt cttcgtgctg tccgctcccg tcgccggccg gctgcgccgc atcgatgccc
     2821 atgtcggcga cccggtcctt gccgacgaaa ccgtcctggc ccgcatcgag ccggccgatc
     2881 cgacctttct cgacccgcgc acggaggccc aggcccgcgc cgcgcggcag gcggcagtct
     2941 ctgcgctcac cctggcaaag gcgcgagtcg aggaagcgcg ggcagagttc gagtacgccg
     3001 acgccgagtt ccaccgggcc cgggaactga tcgccaaggg caccatctcc gtccgcgacg
     3061 ccgaggcggc cgagcgcgcc tacaaggcga ggcgggccgc cctgagcacg gcccaggccg
     3121 cggtgcagat gcgcacctac gagcttcagc aggcagaggc gctgctgatc tcgcccgcgc
     3181 aatcgagaac cagaaccgat gagtgcgact gcgtggacgt gaccgcgccg gtgagcggcc
     3241 aggtgctgca gatcgtcaac ccgagcgagc ggatcgtgac cgcaggcgaa gcgctcgtgg
     3301 agatcggcga cccgggcgac ctggagatcg tggtggattt tctgtccgcc gacgcggttc
     3361 agatcgagcc cgggcagcgg gtcatcatcg agcgctgggg cggcgagtcc gccctggtcg
     3421 gccacgtgcg gcgcatcgag cccttcgggt tccgcaagac gtcggccctg gggatcgagg
     3481 agcagcgggt gaacgtgatc gtggatttca cgagcccgcg caacgagtgg tcgcgcctgg
     3541 gtcacggcta tcaggtcgac gtgcgcgtgg tgctgtggga gcaagacgcg gtgctcgcga
     3601 taccgctgac ggcgctgttt cgcgacgatc agcggtgggc cgtgttcgtc gacgacgacg
     3661 gccgcgccgc cctgcgcgag gtcgtcgtcg gccaccgcaa cgggatggtg gccgagatcg
     3721 tcagcggtct tgcccgggga gaccgggtgg ttctgcatcc gagcaaccgc gtgatcgacg
     3781 gcgtccggat caccggcaga ggctgacgag gccggggggc ctacggcgcc gcagtagagg
     3841 ctagagccgc aggcccagcc ttgctgccag ctccccgagg agagcatcca ggctcaagga
     3901 ttgcgaggtg tgtccgaaca cgtagcggtc gggtcgcacc acggcagcgg ccgagccttc
     3961 gaagagcctg tcgaagcgtc cccgggtggc ttcgagcccc tccagggtcg tggtcgacat
     4021 gccgagggcg tccacgatgg cccgggatgc ctcgctcatg gccagatcgg cctcggacct
     4081 ggcaaccaac gcgaagccgg cacccagccg atcatccagg agatactcct ccccatcggc
     4141 atcccgcacc gcaggctggc ggaaaagata gcccgtggag ccatcctcgc tcacctgatc
     4201 caccatcacc acgccgtcgc gcaggggcgg tgcttcccga ccctggccgt agccggacgc
     4261 gcggttgccg tccggcactt caggcggctt ccgccccacc ctttgtgccg cttccacggc
     4321 ggccagatgc tccatgagct tgccgatggc caccgcccat tccaccaggt cggcggcatg
     4381 agccccccgt tccgcctgat aggaatccag gagaccttcg tctgccgcac cgctcaagac
     4441 caacgggagc ttccaggcca gattgatcac gtcgcgcatt ccggcgttca gcccctgacc
     4501 cagaaagggc ggggtctggt gggcggcgtc ccccgcgagc agcacgccac cctttcgcca
     4561 ggtctcagcg gtggcggcgt gaaactgata cacggcgctg cgccgtattt cgtaggtgcc
     4621 ccgtggtgtc cacggatcga tcagctcgcg gatcctctcg ggctctagca tctcctcccg
     4681 ggtctcgccg ggattgagct tgaactccca gcgccggaaa gggtccttgg tcgcgatgta
     4741 ggtggacagc ctgtcggggt cgcagacctg cagcgtatcc aaacccaggg tgtgcccgtc
     4801 tttcagcacg acgtccacca ccagccagtc gtgatcgtag cccaggtcgc gccagccgat
     4861 ctccagggcc cggcgcgcgc cgctcgccgc gccgtcgcag gcgaccagga accgggcgct
     4921 cgtctcggaa agcgcgccgt ccgcctgccg cgcgcgaatg gcgacttgcc caccggtttc
     4981 ttcgaaggtc tcgacctgga cctcaaggca cccagtcacc ccggagtggt ccatcgccaa
     5041 ccggcggaga tgcgcctcca gctccggctg atcgaacatg gagatgggct gccagccgtt
     5101 gaccccgaac gtggcgtaag gctggccaaa gagccactcg cgtcgggagt tcgcgaaccc
     5161 ggcccgctcg ccgggccgaa ccggctgcag cagagcggcg agctcgccgg cccgccccac
     5221 ccgctgaaag ccgcgaacca cctcgccgtc cagggcgacg gcccgcggca gcgggtagac
     5281 gtgcgcgtcg cgttcgaagg cgatgacttt caggcccgcg tccgcgaaga ggatggcggc
     5341 ggcagcgccc accggcccca tgcccacaat ggcgacgtcg tagctgctca atgtgcgtgc
     5401 tccccgctac ccggaatgag cagatccgca atgacttcgg cgtgtctgcg caccacatcg
     5461 tcgtcgtacg gatcgatgcc tgcaaggcgc tcggcctcgg gagccatgga gaagagaagg
     5521 gatcccgccc ccgtgagtat gtaatagaag tgaacgaagg ggacgttgcg cacggcccct
     5581 gcggatcggg cttcctcgaa cgcggcgccc aaacgctcgt accagggccc gacgatgtgc
     5641 tctgcgagcc actgtagccg ccagtcgtcg tccatgccct cgcggatcat gagccggttg
     5701 gcctcgggga agctcgccga gaagcgcacg aagccggtaa tgagcgcgcg aagctgtgct
     5761 gccgggtccg agccaccttg cccggagacg cgaacccgca acatgtgcgg ggcctggccg
     5821 aaccaccagg tcaccgcctg cttccacagc gcctccttcg tcccgaagtg gtaggcgatc
     5881 agaccacgct tgaccccggc agaggcctcg atctgtcgcg tggaagaccc ctcgtaaccc
     5941 gccgcggcaa aacagcgcac ggcggcgtcg agcaaccgat cccgggtgac ctgggcccgt
     6001 tcctgaaccg gcccgcgatg ggtttgattc ggcatggacg gcaaaacctt gtcatttgac
     6061 gagttattct ataaatcact cgtcattcgt caagttttca tcgaggaggt cgccgtgtcc
     6121 ggcatgaccg acaaactcta cgcgcgcttt cgggtccccg acctgaaccg gcaggcgcgc
     6181 tctcgatctt cccggcggcg gctgggcagc caggtgttcg actactggaa agatcccaac
     6241 ggctttacca tggagcactt tacggacggc gatctgttca acgcatcctc cggatccgaa
     6301 aaatggccgg tggacgtggc tttggggtct cactgaggac cggaaggagc gccttaggga
     6361 aacgtcatga ccagcgcgcg cgagatatac acccaacggg ctgcagagac ctgaagtgct
     6421 gccgggcggt ctgatcttcg gtaccttcct ggtgctcgtc ctggtgctcg ccctggtgct
     6481 cgtcctggtg ctcgtcgccg gctggtacgt gtgggagcgc cggcagcggc gaacgcgcgc
     6541 gatgcccggc ggcctgcacg aggagatcac cctcccccac cggcaagcct gggagctgta
     6601 ccacaacaac ctgtcgctct gctcgaagaa gacgcgggcg tgtttgagcg agttgtccat
     6661 cgactatgaa agccaccaca tcgacctcat cgagaccggc cgttacgaga acgtctcccg
     6721 ggagttcctg aaggtgaatc ctgccgggct tgttcccgtg ctcgtgcatg aggggcaccc
     6781 ggtgtacgaa tctcacgcgc agatcgcctt cgccgcggcc cgcagcgacg acccccaccg
     6841 cctggttccc gccgaccctg accagcgccg tcagatggac gattgggtgc gcaagacatc
     6901 gctcatcggc gacgaccccg tcggcgcggt tcaccagacc gccggcaatg ccgtccccgg
     6961 tctgacgctt cccctgttcg cgaccatgct cgaacggatc ccgctgcggc gggttctcga
     7021 gggcctgctc tttcaccgac tgaagcggcg cgccttcatc ctcatgatca tcaagctata
     7081 cggcatcgac agcctgacca gcgcaaagcc actgctccgc gtggtccagg ccagccgcga
     7141 cgccatgcac cgccacctcg atgacctgga agcgaccctg aaggacagcg gcgggccctg
     7201 gatcgtcggc gagcacttca gcctcgccga catcggcatg atggccatcc tggagcgcct
     7261 gcgcgaggct gactggctcg acgtgctggt gtcaggccgg cgcccccggg tggccgccta
     7321 ctggcgcgcg ctgcagacgc gcccgagcta tcagtcggca atcgtcgcct gccggggcga
     7381 ggcgatcgaa cgcggtaccg ccaggctcgt ggccgcaaag cagaacaacc ccgtgctgcg
     7441 cttcgccttg atcgagacga tcacctgaac gcgcggccat ccatcagaca cgacaatcac
     7501 gaaagcgagg acaccatgaa gctagttagt ttttccacga cagagaatcc gaaaccccga
     7561 ctcggcgccg tgcgggacgg cgggatagcg gatctgagcg gttcggacgg gatcccggag
     7621 acgatgctgg gtcttctgca tgccggggac gctgccatga aggccgcaca cgaggccgct
     7681 gaagcggccg ctgacaccgt gccgctcgat caggtgacgc tccacccgcc ggtgctgaca
     7741 ccgggaaaga tactcgccat cgggctcaac tacggcgacc acatcgagga gagcggcatg
     7801 ggcaaaccca agcatcaggt gtggttcaac aagcagcaca actgcgtcaa cggaccctat
     7861 ggcgacatcg cgctgccgtc ggtctccgag atgctcgact acgaggccga gctggtggtg
     7921 gtgatcggca agcgctgcaa acacgtgccg cgagagcgcg cggcggaagt catcgccggc
     7981 tactgctgcg gcaacgacgt gagcgttcgc gactggcaga tgcgcgccca gaccatgcag
     8041 atcggtaagt cattcgacac ccacggcccc accgggccat ggctggtcac gccggatgag
     8101 gtgggcgacc cccacgacct ggacatccgc gccgaggtca acggggaact ccgccagaac
     8161 tcgaatacga agcacctgat ctacgactgt tacgacgccg tagcgcatct gacccaggcg
     8221 ttcaccctgg acccgggcga cctgctgttc atgggcacgc cggccggtgt cggggcggcg
     8281 atgaagcccc cgcagtttct caaggccgga gacgtggtca gggtggaaat cgagaagctc
     8341 ggctacctcg aaaaccgggt ggtccccgaa gcagccgagt gcgtgatcga gtgacctagc
     8401 ggggatcgag cccgcgcttg gcgcgctcgg cgttcatgcg ctccttgtcg acgccgagcc
     8461 gcgggccgag gtcttcctct ttgaccgtcc cggccatgtg cccgccggcc agcttgccgt
     8521 tgtacatgat cttcttcacc ggcgtgacgg caagcacggt ccgcaacggc gagtcgagca
     8581 ggttgtagaa gaactgctcg ccctccgggc tgttgggatt gagcttctgc gacagcgcgg
     8641 tgtagaacca cttcttggtt tcctcgtcgt cgaagaactc tccggtgccc ttgaaggtga
     8701 tggcaccgcc cggcagctcc tctgaagcgc ccggcgggta gccgcagctg ctgacgttga
     8761 cggaaacgcg gttatcgcgg cggatggcaa ccgttctatg gcgatgcgcg gcaaaggtca
     8821 gccagatctt gccgtcgtgc cacacgaacg cgtgggtgac ccctaccggc cagccgtcct
     8881 tggttgccca catgagcacg cattcgacgg agttgttcat cagctggtcg acttcctcgt
     8941 ccgtaaacgg gtaaatcgag acgatctcgt gatcgtgagt ttgcgcttga gccataagtt
     9001 ccccctggag taactgccgt ccccgatccc ggggaaaaag agcggctaga ataatgcaat
     9061 cgctcgatcg gcgataccga cgagccccac cggcctgctc gcgccgatcg gaaacagcaa
     9121 cgtcattccg cttaaacttc aaccggtaaa ggagcatcca cggtaacgac catgagcaac
     9181 ctgcgccgat cgacccaact ctacgaacgc gccaaggccg tcattcccgg gggcatcccc
     9241 ggcatccggg cgccggaaaa tttcgtcccg ggtgcttacc ccatacttct ggccggaggc
     9301 cgcggcggtc acgtcacgga cgtggacggc aacgactatg tggacttgct gctggggtac
     9361 ggacccgtga tcctcgggca cgccgagcag gccgtggaca gcgcggccca cgaacgcgcc
     9421 gcccacggct tctgcctgga tctccccgaa cccgtcatga tcgagctggc cgagcagctc
     9481 gtatccatgg tgccctctgc agagcaggcg ctgctgctga agacgggctc cgatgccacg
     9541 agcgctgcga tccgcatcgc caggacccac acgggcaggt ccgtggtcct gcgctgcggg
     9601 taccacggct ggcacgactg gtgtcttcag ggagatcccg gcgtgccgtc cggcaccacc
     9661 gacaccgtca tcgcgttccc ctacaacgac ctcgaccgcc tggccgctct gctgacggcc
     9721 cactcgggcg acgtggcgac catcatgctc accccggtgg gtcacgagtt cgaccgccag
     9781 atcgagcaac ccgaaccagg ttttctggag ggggtgcgca gcctggccga ccagcacggc
     9841 gtggtgctcg ccttcgacga ggttcgcacg ggcttccgcg tccacccggg cggcgcccag
     9901 ggcctctacg gcgtcatccc cgacctgacc gctctgggca aggccatgtc gaacggttac
     9961 gcggtgacgg ccctggtcgg ccggcgggac gtcatgcaga gcgcccggag cacgttcatc
    10021 tcatccacct acttccccaa cggcatggag atggcggcgg ctctcgagac cctgaagctg
    10081 atcgccgagc acgacgtgct taccggcatc gccgctaccg gcgctgcgct cgccggaggg
    10141 ctgtcggaaa tcgtcgcccg gcacggcctg cccgtctccc tgtcacccta cgacgagatg
    10201 cccttcctcc atttcgaccc ggacctggca tcaggccagc ccgagcgacg agacgctttc
    10261 tacggcgcca tggcccggag cggcgtattc gcccacccgc gccatcacgg atttctctgc
    10321 tggcgccaca gcgccgacga cgtggcgcgc gtgctagagg cggtggatgc cgccgcggcg
    10381 gcgctgggaa gctagtccgg cccctctggc gggcgggcga tgcccgacca gtttcccgcc
    10441 agccggtcgt ccgccgccag gcggacgtcc acgggcaggc cggggagttg ggacattccg
    10501 gaaaacgggt ccacgtgggc agcacccgag cttgcgagta tgttcacgtt cacgccgagg
    10561 ctgccggccg caacgcgctg gccgctggcc tgatggcccc agccgtgggg gaccgccacc
    10621 acgccggcca gcaggtcgtc cgacgtccgg accggcaggc ggatggcgcc cgcggagctg
    10681 cgcacgtcgg ccaggtcgtc gggcttcaag cccagggcgg cggcgtccgt ggggttcatg
    10741 aagacgtagt tgcggccctg gtcgccgcgc agaaacccct cgtagttgtg agtccaggaa
    10801 ttgtgcgtgg tgacctcccg tttggttatc agccggaagg gaaactcgct ggcagaccct
    10861 tgctcgcgag ccgaggcgag ggtggcctgg agcttcgaga cgagctccgc gggcgcaagc
    10921 tccacccgcg ccgagtcggt gtagacccgc gcgccgagga aatcgcctcc gacgttggga
    10981 ggccgcagcc gaccgtgacc gagcagccgt cggaaggacg gctgccgcga gagcttgagg
    11041 atcaggttca gcagcaggcg ctggggcacc tcgtcgcggc cggtgagcgc gcggttgcag
    11101 aatttcagcg cccgcatcac acggtgaaag cccggcgagc cgaacagagg cgcccctgcc
    11161 cgggtgccta gctccgtgta gatggttgcc tcgtcgcggg cctcacccgg ggcgtcgacc
    11221 acggggttcg tcgcctgcag gtagggccgc gcctgcatcc cgagcaccag cgggaacacg
    11281 aacggcagat ccggacgttc caggggggac gtgcagggaa gcatgtaatg agcggccgcg
    11341 ccggtctcgg tgggcaggat atccagcgtc accagcagct cgagctcgcc aaaggcctgc
    11401 ttgaggcgtt cgctgtcggc catggtgagc aggggattgc cgcccgtgac gatcagcgcc
    11461 cggatctgcc cggggccggg cgtcagaatc tcgtcggcga gaataccgcc gggaaaggca
    11521 tcgttcgtct tgcgaaagcc accgacgcgc gagcggtcgt ccttcaccag cagaccgtac
    11581 ttcttgccga accgggcgaa gtcgaagatg ccgttgccaa cgagggtgcc gccgcgcgcg
    11641 tcgaggttgc cgctgaccgc gttgataacc tcctggatcc agaagcacag ggaaccgtgc
    11701 gctcccatgt tgacgccggt ggacgagtaa agggcggccc catcggcggc aacgtagtcg
    11761 acgacgagct ggcgaagcac ttccgcgctg acgccagtgt gttgcgccgc acgctcggcc
    11821 gtccagggct cgaccagctc gcgcacctct tcgaacccgt tcatgtgagc gtcgacccga
    11881 tcctcgcgca ccccgcccgc cgcgatcagc tcatggagga aggcgaggta gaagtagaga
    11941 tcgctgttgg gccggatgaa gacgtgctcg cctgccgctt tggccgtctc cgtgcgccgc
    12001 ggatcgacca ccacgaccct gccgccgcgc tcccggatgg cgttgaggtg ccggcgcggg
    12061 ttggggacct gcagaaagct ccacttggaa accatgggat tcgcgccgac gatcacgagc
    12121 agcttcgtgt tgagcagatc cgggaacggc tgcgtgaacg gaaagccgta tatgcgctcg
    12181 gccaccgcga acttgttggc gcagtcctgg gtcgcggagg agtacatgtt ccgcgagccc
    12241 aacccatcca tgaagccctg ggcaaatacg gggtgaagca cgccgaaccc cgccgcggtg
    12301 cccacgtaca tggcgatggc atcgggcccg tgctcgtccc gaatgcgcgc gagctcgctg
    12361 ccgatgtcgc cgagggcctc gttccacgag acgtcgcgcc agtcgccgcc caccctcttg
    12421 cgagggcgca gcaaacggtc gggggagccg tataggtgat gctggcgaag gcccttcggg
    12481 caggcgaacc cgccggttgc cacatgatcg ccgtccggac ggacgtccgt gaccacgcca
    12541 tcccgggtgc tgacacgcaa tccgcacagc gactcgcaaa tgcggcagaa ggtgaggtgc
    12601 gagcgctcgc cggcggtaac cgccccgtct gtggtcatga gtcagcgctg ccggtcagtc
    12661 gtagcggatc gggtccttgc cgtcccagtt ctcggcggcc ttcgcgatct gcgcgaagcg
    12721 ctcgatgatt cccggtcccc gcgtgaccac ctcgagcatg cagcccatca acggccgcgt
    12781 atcgtagtag gcgaactcga tgtccattac ggcgccgcga ttggctgtcg ggtagccgag
    12841 actctcgaag tactcggagt ccgcgtcgat gtcgtcggtc cagacgcaca tatggtgaaa
    12901 cgcgtcggtc ccggcaggca cgctgtcgcg ataggcgcag cggtcgacct tgggctggat
    12961 aatttccacc tgcaccgggc ccgcctgggc gatggcgacg atcatgtcga gcttggccgg
    13021 ctctccccgg tagatgatct gctcgaaggc gttgtcgtac tcgttgacga aaaacgggcc
    13081 gacgcccatc tgctccaccc atttcatgca ggcggcttcg acgtcgtcga ccacccacgc
    13141 attttgaaaa atgctgcggt ccgttggctt gtccagcatc tcctccctcc agattcgagt
    13201 gcgcgctgag agtagcacat cgaagcgccg cacgcggcgg ccaagcgcgg gggcacgtca
    13261 tcgtgaacca ccgctgcagc gccaggtttg aaactgccgg cgtacgtagt agcttgggct
    13321 caattgaacg ggcaatcctc gagctgagga ggacgggttc catgatcgac gtcgttcgca
    13381 cgccggaaga acgtttcagg gggctccccg actttcccta cgaccctcgc tacgctgcgt
    13441 ggagcggcct gcgggtcgcc cacatcgacg agggccccgc ggacgccccg gtcgcgctgc
    13501 tgctgcacgg cgagccgacc tggagctacc tgtaccgcaa gatgatcccg ccgctgctgg
    13561 aggccggcta tcgctgcgtg gcccccgatc acgtggggtt cggccgctct gacaagcccg
    13621 tggaggacga ctggtacgtg atcgagcggc acgtggagcg gatcaccttc ctcatcgagt
    13681 ccctggacct cacggacatc accctgttct gtcaggactg gggcggcccc acgggcttaa
    13741 gacaggccgt ggacatgccg gagcggttcg agcggctggt gatcatgaac acctggctgc
    13801 atcacgaagg ctacgagtac tcaccggccg tccgcttctg gcgggaggcc gccacgaacc
    13861 gggcctggct cggctggacg ggcggcgacc tgccctgcgg cgccatcgtc gcgggcacgc
    13921 tggcccgcag cgggctggac cgagcggccg tgaagcgcgc ttacgacgcg ccattcgctg
    13981 agggcggccg ctccaaggca ggcgcgcgcc ggttcccatg gtgccttcca ttcggcgagc
    14041 cggaggccgg caacgccgct gatcaggcgc gctgcttcga agcgctaaaa agctgggcca
    14101 agcccgccca tttcattttc ggcgagaggg acccgatatt caccacgggc tgggccaggc
    14161 aatggtcggc gctgctgccc aacgccaccc tcgatctgat ccccgacgcc agccacttcg
    14221 tgcaggagga ggccggcgag gagatcgtcg ccgcatttct ggagcgcacg cgctcgacgt
    14281 gatcggcacc gcgcgtctag catggcctgg cctgacgaac ccagacgagc cttcagggag
    14341 atctcatgat cgacatcaac gaccgcatgg ccattctcga cctttgtgcg agctacaact
    14401 acctcttcga cacctgcgcc gcgaaggagt gggcggccac gttcaccgcc gacggcgtct
    14461 tcgaaggccc ggccggacgg gcggagggtc cggaggacct cgccgagttc tgccggaaca
    14521 ccaacgcgca gtacccgggc gccatgcact tcaccgacaa ccacctcttc gagcagaacg
    14581 gcgacacggt caggcacaag tgcttcctga gcatgcaggt tccctccccc gagggcaccc
    14641 agaccctact gctcacctac gaggacgagc tgagacgggt ggacggcgag tggcgcttcg
    14701 cctcacgcct gtgccagccg ctgagcgcat agcctgacga ccactaaggg ctggaagtgg
    14761 agcacgccgc cggatccgcg tccgtcttgg cgtggcggcc ggcgaagtcc cacatcatcg
    14821 cctccatgcc gccaaagccc ggacaggagt gccccccctg gaagaagcag gtcgcgacct
    14881 cggaggcatg gccaggcgtt tcaccctcgt cctgggcctg ccagacctgg caatcgagac
    14941 tgttcgagta ggcgcgcgcg taggcgtcgg cttcttcgac aggcagcggc gccgcatcgt
    15001 gcatcgatgc ccactcgtac ccgatgtcgc tcgccggcac gtaaagccag ccgttgaagc
    15061 tcgtgtcgag agcaacctgg ggcgacgtcc caggaaagtt ggcacgaccg ggcaccgtcc
    15121 ggtccgtctc tccccagaac cccagcaggt gacggggccg gtccacgagc tcgcttgcgg
    15181 ggtcgtactg ccggaaaccc ggatggggcg cgcccacctg aggcatgaag ccggcaaacc
    15241 gggtgcgcgt tcgcgcatcc ttggcgagct cgtagaccat catcccgccg ttggagcagc
    15301 cggtcaccca gatcatgctg cggtccacgc agtaaagcgc ctcgatctcg tcgagcagcg
    15361 ccgcgatctg gcccaccgag tcgcgacagg tggtccagtg gcaggtgtag tcgccgttgt
    15421 actcagcgcc ccaggtattg tcaccctgac tgccgtccca gacggggcag ttccccggct
    15481 cggctcccgc tccgtagagg gtcctgcagc tgtcgtagca gtagtcgtgg ccggcgttgt
    15541 cgagatccac gcaggtggac ggcccattcg ccgagtccag ctcgggcggc ccggcatcgc
    15601 agtcggaggc gaggtatccg tccccgagcg cgcacacggg cagccgggcg ctgcgatagc
    15661 cgttccagga attccggccg ccgtcgccgt agccggtggg ggtcaccagg atgtagccgt
    15721 tgttcttggc atgacgggcg gccgacgccg gcagggatcg aaagctccca ccccagccgt
    15781 gcaggtagac gagcaccggg aacccgccgg gggagttgac gtcgtagctc gacggcagcc
    15841 g
//
LOCUS       DBNHLM010000200         2230 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1284129, whole genome shotgun sequence.
ACCESSION   DBNHLM010000200 DBNHLM010000000
VERSION     DBNHLM010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1284129"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="AC16QF_09135"
     CDS             complement(<1..525)
                     /locus_tag="AC16QF_09135"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6448764.1"
                     /translation="MYDRATTYGSDRVHEGPCHEHGPHGCPCCAATLGELLRRVGLDV
                     EAQAGRSVPAAPRAPVPTLFCNARVLTMDDAGSEFDTLLISDGRIQWVGNADADAVPE
                     VGDDTVRVDCNGRTILPGFVEPHMHLSAIAMLRRFENVGPFRYPTVSGALARLKEVAA
                     DTDPGAWIIGRQFDP"
     gene            651..785
                     /locus_tag="AC16QF_09140"
     CDS             651..785
                     /locus_tag="AC16QF_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448765.1"
                     /translation="MTTGMTVMIVMTTLAVTVGVVALRVSWRIVNRLSEERPQNEEER
                     "
     gene            923..1213
                     /locus_tag="AC16QF_09145"
                     /pseudo
     CDS             923..1213
                     /locus_tag="AC16QF_09145"
                     /inference="COORDINATES: protein motif:HMM:NF041106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1244..>2230
                     /locus_tag="AC16QF_09150"
     CDS             1244..>2230
                     /locus_tag="AC16QF_09150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6448766.1"
                     /translation="MKFGIFYELQLPKPWEADSEYRLFNNALDQIELADRLGFDYAWE
                     VEHHFLEEYSHSSAPEVFLAAASQRTRNIRLGHGIVQLPTNHPARVAERVSTLDLISH
                     GRVELGLGEGSSVTELHPFGRRFREKRAVWEDAVRATLPMFWEEGWEYHGEFFDFPLR
                     NVIPKPRQQPHPPLWVACSQMDTIRMAGRRGMGALGFSFVSPESAQAWCHAYYNEFVK
                     RQDRLCDYQPNANLAVVSPFMCCETDEEAQEKMDGWSFFQFALILYNKEGPFEPGTVN
                     LWDRYQEWKQSGKGRDLTPNSGLVGSPETIRTRLQQFQDVHIDQVILLNQAGK"
BASE COUNT          432 a          721 c          730 g          347 t
ORIGIN      
        1 ggggtcgaac tgtcggccga tgatccacgc gccagggtcg gtatcggctg cgacttcctt
       61 gagcctggcc agcgctccgc tcacggtcgg gtagcggaag ggccccacgt tctcgaaccg
      121 tctgagcatg gcgatggcgg agaggtgcat gtggggttcg acgaaccccg gcaggatggt
      181 tctgccgttg cagtccacgc gcacggtgtc gtcaccgacc tcgggcacgg catcggcatc
      241 ggcattgccg acccattgaa tgcgcccgtc ggagatcagc agcgtgtcga actccgagcc
      301 cgcatcgtcc atggtcagaa ccctggcgtt gcagaacagc gtgggcacgg gcgccctcgg
      361 tgcggccggc acggaccgcc ccgcctgggc ctcgacgtcc agccccaccc ggcgcaggag
      421 ctcgcccaga gtcgccgcgc agcagggaca gccgtgcggg ccgtgctcgt ggcagggacc
      481 ctcgtgaacg cggtctgatc cgtaggtagt tgccctgtcg tacacgcgca tctccccccg
      541 aggccacaag cgcatagatt acgctatttg catggagccc acattgggaa cactggaccc
      601 ggagcgcgca atgaggcacc gtagcaacca cggtatcggg aggaagcacc atgactaccg
      661 ggatgacggt gatgatcgtc atgaccacgc tggcggtgac cgtcggcgtc gtcgctctgc
      721 gcgtgtcgtg gcgtatcgtg aaccggctgt cggaagagcg gccgcagaac gaggaggagc
      781 gctgaccgct atcccagcga cggccatcag gggcccgtcg gcgcgatccc ccataccgca
      841 acgagtctga atcaacggag ggacgaccgt ggcaaagcgc gtcgactggt attactaccg
      901 caacggctga accagctgta ccagagcatc caagttcttg gaggcaagca aaatagagat
      961 caaggaccgc acgccggcca gccagaagct gacggaaccg gacgcccgcg agctgctcgg
     1021 caaagcgtcg aaaatgatcg tcgcgaaggg taagaaaatc agcgagttcg gcggtggtaa
     1081 agcggccacc gacgacgcgg tggcggccat gctcggaccg accggcaatc tgcgtgcccc
     1141 tacgctgaaa gtgggcaaga cgctgctggt cggcttcaac gaggaggcct acggcggcgt
     1201 gttcggcggg taggaccggc agcggccgga gagggaggcg aggatgaaat tcggcatctt
     1261 ctacgagctg cagctgccaa aaccctggga agcggacagc gagtaccggc tgttcaacaa
     1321 cgcgctggat cagatcgagc tcgccgaccg gctgggcttc gactacgcgt gggaggtgga
     1381 gcaccacttc ctcgaggagt actcgcactc ctccgcacca gaggtctttc tggccgcggc
     1441 cagccagcgc acgcgcaaca tccgcctggg ccacggcatc gtgcagctgc ccaccaatca
     1501 ccccgccagg gtggccgagc gggtctcgac cctggacctg atcagccacg gccgcgtgga
     1561 gctcgggctg ggcgaaggct ccagcgtcac ggagctgcac cccttcggcc gtcgtttccg
     1621 cgaaaagcgg gcggtctggg aggacgcggt aagggccact ctgcccatgt tctgggagga
     1681 gggctgggaa tatcacggcg agttcttcga ctttcccctg cgcaacgtga ttcccaagcc
     1741 ccgacagcag ccccacccgc cgctgtgggt cgcctgctcc cagatggaca ccatccgcat
     1801 ggccggccgc cgaggcatgg gcgcgctcgg cttttcgttc gtgagcccgg agtccgccca
     1861 ggcctggtgc cacgcctact acaacgagtt cgtcaaacgt caggaccggc tctgcgacta
     1921 ccagcccaac gccaacctgg ccgtggtctc tcctttcatg tgctgcgaaa ccgacgagga
     1981 agcccaggag aagatggacg gctggtcgtt cttccagttc gcgctcatcc tctacaacaa
     2041 ggaaggaccc ttcgagccgg gcaccgtgaa tctgtgggac cgataccagg agtggaagca
     2101 atccggcaaa ggccgtgacc tgacaccgaa ctcggggctc gtgggctcac cggaaaccat
     2161 ccggacacgt ctgcagcagt ttcaggacgt gcacatcgac caggtcatcc tgctcaatca
     2221 ggccggcaag
//
LOCUS       DBNHLM010000201         5344 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_267685, whole genome shotgun sequence.
ACCESSION   DBNHLM010000201 DBNHLM010000000
VERSION     DBNHLM010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_267685"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..667
                     /locus_tag="AC16QF_09155"
     CDS             110..667
                     /locus_tag="AC16QF_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0008289 - lipid binding [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HIR6448767.1"
                     /translation="MGNLRLTGLRRAIGALCVAACCAACTGIPDGVEAVTDFQLERYL
                     GTWYEIARLDHTFERGLTRVTAEYARRSDGGISVTNRGYDGALGEWREARGKAYFLGN
                     PATGSLKVSFFGPFYGGYHVIALDSEGYQWAMVAGPSRSYLWVLARDAVVPDETFNRL
                     VARAAELGFPTENLIFVDHSGANAH"
     gene            1022..1624
                     /locus_tag="AC16QF_09160"
     CDS             1022..1624
                     /locus_tag="AC16QF_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448768.1"
                     /translation="MTWTQILATYLVVGGTVLLFTPVRPTLARHLNLYEQRHISRLAF
                     VVYAGIVVASDLLLWPFLAAKHRPSRRRVNKPEHAVLDPELIEEQKILRDSLGQMGDD
                     GVNNDELPAATGDFGREASNPIPCNTVFGSTAYLARLRFSDGAKILFDRLGSVSSDAS
                     RYPVDCYAIKHPDGRGLGVFYISPYHKRNSRKAPEGFSID"
     gene            complement(1631..4060)
                     /locus_tag="AC16QF_09165"
     CDS             complement(1631..4060)
                     /locus_tag="AC16QF_09165"
                     /inference="COORDINATES: protein motif:HMM:NF039587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HIR6448769.1"
                     /translation="MTRCRVLMVALALIVAGRSLAESTAGAAQSRSAELNTGEVVDIP
                     YRFHEQASIRVDGRLDDAAWADATVIRDFIVIDPDTMEKPRWETKLMLIYTERGIYAG
                     YDLEQPRDMIVSVLSPRDTGTNFGDFVGLVLDTSGDGKYGYWMSLAASGVKTDGTLIP
                     EAQFNRDWDGVWYGETALTEKGWSAEFFVPWSQLAMPKEAGTRRMSLFASRRSVAIGE
                     RWGVPALPFTITKWIQYMRPLEMEGVDPRQQWSVIPYTSVTQDQVEKETDFRAGAEVF
                     WRPSSNFQVSASVLPDFGNVESDAVIVNLSALETFFPEKRLFFLEGRDIFLTSPRADA
                     SKNFFPVTVVNTRRIGGSPRPPDVPEGVAIPSRQLDQPVELYGAVKATGQLGSLRYGL
                     LGAMEDDVTFKVDDLRFEQSGSEYGVARLLWEDVSYGSYRALGTISTLTAHPEEDAVV
                     HGIDYHYMTGNGSWKLDGQVLYSDKDSVGDGWGTFADAVYTPRRGLKVNFEFEYYDDK
                     LDINDLGFLQRNDITRGGIGVDYTNPSVGWARQLNLSGLSIYEVNGDGDVTRQGASAS
                     TNMLLNNLGRVRASALFFGRRTDDLNSFGNGSFVIPSRWEGNVDYFSDPSRSLSYRVG
                     FDLDEETVHGMAIKARLGATWRPSQRMKLDLFTEYVRRDGWLLHQDAPEDFDGPNNHF
                     TTFDTREWRPTLGIDFFFTAKQQIRLSAQWVGIQAREQRFFQIPENEGELDEVDKPWE
                     QSDDFTISNLNVQLRYRWELAPLSDLFVVYTLNGAQGPPRASFADLLESSFSDPVFEK
                     IVVKLRYRFGS"
     gene            complement(4071..4256)
                     /locus_tag="AC16QF_09170"
     CDS             complement(4071..4256)
                     /locus_tag="AC16QF_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448770.1"
                     /translation="MADVLPFKKRSLKEKAKGRTLCARGFHKWVVDKQQRFDVKQGGL
                     VTVHRCQRCGATKNAAI"
     gene            complement(4381..4737)
                     /locus_tag="AC16QF_09175"
     CDS             complement(4381..4737)
                     /locus_tag="AC16QF_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6448771.1"
                     /translation="MVSIAVVDDDVAILDAMTLLAESRGWLAKVFASGEAFLDAFEHD
                     RDIDCLVLDPHLQGIGGDDVALALADTGVPIVGLTARPESPVTQSILESGAAAILTKP
                     VRPAELVSAIENLLLN"
     gene            complement(4808..>5344)
                     /locus_tag="AC16QF_09180"
     CDS             complement(4808..>5344)
                     /locus_tag="AC16QF_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6448772.1"
                     /translation="SIVKQVASLLDCPLEVASTVGEGSCFSILVPLGLLDEEETVPGQ
                     PQTGQLAPGEPVILFVDDDPAIVDATTMMLELAGISVRSALDGNRAIAMLEEGLSPDM
                     VVSDFRLPGANGIEVIRRARELRGSYLPTVLMTGDTSGKVIDEAAISNCTVLRKPVDT
                     NHLISLIERGLDAGRGPG"
BASE COUNT         1022 a         1738 c         1610 g          974 t
ORIGIN      
        1 tttttagctt gttggacccc taaactatca acgaaaccag ccccggtctg tcgtaaaaat
       61 gcaattgaat tcaacttcga aaatgagcgg cgccgcagag cacatcacat tgggaaatct
      121 caggctcaca ggtctgcggc gagcgatcgg cgcgctgtgc gtcgctgcct gctgcgcggc
      181 atgcacaggc atcccggacg gcgtcgaggc cgtgaccgac ttccagctgg agcgctatct
      241 gggcacctgg tacgagatcg cacgtctgga tcacaccttc gaaagggggc tcactcgagt
      301 gacggccgag tatgcccgcc gaagcgacgg tggcatcagc gtgacgaacc gcggctacga
      361 cggcgccctt ggcgagtggc gggaagcccg gggcaaagcc tacttcctcg gtaaccccgc
      421 caccggcagt ctgaaggtgt cgttcttcgg gcccttttac ggcggctatc acgtgattgc
      481 tctggacagt gagggctacc aatgggccat ggtcgcgggc ccgagccgtt catatctctg
      541 ggttctcgcc agagacgccg ttgtgccaga cgagacgttc aaccgcctgg tggcgcgcgc
      601 cgccgagctc ggcttcccaa ccgagaatct catattcgtc gatcactccg gcgcgaatgc
      661 ccactgaaag acgggcggta agggcgaccc gccgggctga tgacccaatc tggccatggc
      721 catgaccggc tgttaggcat ctttctacaa atcgctcaca aaacggcgtt ccttcgacgc
      781 cgcgccacgt ctaacctact tgaatgatcg gggggccaga atacgacaca ccagcgcacc
      841 ggcgttggga taggggcagc gaaagcgacg gcccgatgcc cacccgccat cggcgctcac
      901 acggcggtcc cgacaggaac ccaagcacgc tgtacgaccc ccgccaactg gctttgctgc
      961 ctgaactcca gggcgatctc ttcgaggagc tgtccagttc catggtaagc gggcgatgac
     1021 gatgacctgg acgcagatcc tcgcaaccta cctcgtcgtc ggcggcaccg ttctcctatt
     1081 caccccagtc cggcccacgc tcgcgcgtca tctgaaccta tacgagcagc gccacatttc
     1141 ccgcctggcg ttcgtcgtct atgccggtat cgtcgtggcc agcgacctgc tcctctggcc
     1201 gtttctcgcc gccaaacatc gcccctcgcg ccggcgggtc aacaagccgg agcacgcggt
     1261 cctggacccg gagctgattg aggagcagaa aattctgcgc gactcactcg ggcagatggg
     1321 cgacgacggc gtcaacaatg acgagcttcc ggcagctacg ggggatttcg ggcgcgaagc
     1381 atcgaacccg attccgtgca acacggtttt cggcagcacc gcgtacctgg caaggctgag
     1441 attctcagac ggtgcgaaga tactcttcga ccgcctgggc tctgtatcct cggacgcctc
     1501 tcggtacccc gtcgactgct acgccatcaa gcatcccgac ggccgcggcc ttggcgtttt
     1561 ctacatttcc ccctatcaca agcgcaactc acgaaaagcg cccgagggtt tctcaatcga
     1621 ctgagcggcg tcagcttccg aaccgatagc gaagcttgac cacgatcttc tcgaacaccg
     1681 ggtccgagaa cgatgactcc agcagatccg caaagctcgc cctcggcgga ccctgcgccc
     1741 cgttcaacgt gtagaccacg aacaggtccg acaacggggc gagctcccag cgataacgca
     1801 gctgcacatt caggttggat atcgtgaaat cgtcgctctg ctcccaaggt ttgtcgacct
     1861 cgtcgagctc gccttcgttc tcaggaatct ggaagaacct ctgctcacgg gcctgaatgc
     1921 ccacccactg cgcggagagc cggatctgct gctttgccgt gaagaagaag tcgatgccca
     1981 acgtggggcg ccactcccgg gtatcgaacg tggtgaagtg gttgttggga ccgtcgaagt
     2041 cctccggagc gtcctggtgc agcagccaac cgtcccgacg cacgtactca gtgaacagat
     2101 ccaacttcat tcgctgggac ggccgccagg tcgcgccgag cctggccttg atcgccattc
     2161 catgaaccgt ctcctcgtcc agatcgaagc cgacccgata gctgagcgaa cgggagggat
     2221 cggagaagta atcgacgttg ccttcccacc ggctggggat gacgaaggag ccgttgccaa
     2281 aactgttgag atcgtcggtc cggcgaccga agaacagcgc ggacgcccgc acacggccca
     2341 gattgttcag caacatgttt gtcgaagcag aagccccctg ccgggtgacg tccccgtcgc
     2401 cgttcacctc gtaaatcgac agcccgctca ggttgagctg ccgggcccag ccgacgctgg
     2461 ggttggtgta gtcgacgccg atgccgcccc gggtgatgtc gttgcgctgc agaaagccca
     2521 gatcgttgat atccagcttg tcgtcgtagt actcgaattc gaagttgacc ttgaggccac
     2581 gacgcggggt atacaccgca tccgcgaacg tgccccatcc gtcaccgacg ctgtccttgt
     2641 ccgagtaaag cacctggccg tcgagcttcc acgagccgtt tcccgtcatg tagtgatagt
     2701 cgatcccatg cacgacggcg tcctcctcgg gatgggccgt gagagtggag atggtgccca
     2761 gagcccggta acttccgtag gaaacgtctt cccagagcag gcgggcaacc ccgtattcgc
     2821 ttccggactg ctcgaacctc aggtcgtcta ccttgaacgt cacgtcgtct tccatggcgc
     2881 cgagcagacc gtagcgcaat gagcccaact ggccggtggc cttgaccgct ccgtaaagtt
     2941 cgacgggctg gtcgagctgc ctggacggga tcgcaacccc ctccggcacg tcgggcggcc
     3001 gcggcgaacc gccgatgcgc cgcgtgttca ccaccgtgac cggaaaaaag ttcttagacg
     3061 cgtcggctct cggcgaggtg agaaaaatat cgcggccctc caggaagaac agccgcttct
     3121 cggggaaaaa agtctccaga gccgaaagat tcacgatgac cgcgtccgac tcaacgttgc
     3181 cgaaatcggg caaaacggat gcggaaacct ggaaattgct ggacggccgc cagaacacct
     3241 cggcgccggc ccggaaatcg gtctccttct ccacctgatc ctgggtcacc gaggtgtaag
     3301 gaatcacgct ccactgctgg cgcgggtcca cgccttccat ctccagcggc cgcatgtact
     3361 ggatccactt ggtgatggtg aaaggcaggg cgggcacgcc ccagcgctcg ccgattgcca
     3421 cggagcgacg cgacgcaaag agcgacatgc gccgggtgcc cgcttccttc ggcatcgcca
     3481 gctgcgacca gggaacgaag aactccgccg accacccctt ttcggtcagg gcggtttctc
     3541 cgtaccacac tccgtcccag tcacggttga actgagcctc gggaatgagg gtcccgtcgg
     3601 ttttgacccc gctggcggca agggacatcc agtatccgta cttgccgtcg ccggatgtgt
     3661 cgagaaccag gccgacgaag tcaccgaagt tcgtccccgt gtcccgcggc gaaagaaccg
     3721 agacgatcat gtcacgcggc tgctccagat cgtagccggc gtagatgccg cgctcggtgt
     3781 agatgagcat caacttcgtt tcccagcggg gcttttccat ggtgtccggg tcgatgacga
     3841 tgaagtcgcg gatgaccgtc gcatccgccc aggccgcgtc gtcgaggcgg ccgtccaccc
     3901 ggatggacgc ctgttcgtgg aacctgtaag gaatgtcgac gacctcaccg gtattgagct
     3961 cggcagacct ggattgcgca gcgccggctg tggattcggc caggctacgt cccgcaacga
     4021 tcagggctaa agccaccatg agaacgcggc accgggtcat gctggggcgc tcagatggcc
     4081 gcattcttgg tcgcgccgca gcgctggcag cgatggacgg tcaccagccc gccctgcttg
     4141 acgtcgaagc gctgctgctt gtcgaccacc catttgtgga atcctctcgc acacaaggtc
     4201 cggcctttgg ctttctcctt gaggctgcgc ttcttgaacg gcaggacgtc agccatcgcg
     4261 gctcccggat tctgcaaaag ttgaccggcc cgacacaccg gcatggggtt cgccgggggc
     4321 gaattgttgc agctttgcag gttcaacgct cgccgatcgt tgcccggcgc agcaaggccc
     4381 tcaattcagc aacaggttct cgatggcact taccagctcg gcaggcctga ccggcttggt
     4441 gaggatggct gccgccccac tctcgagaat cgattgagtc acggggctct ccggtcgagc
     4501 ggtcaggccg acgatgggta cgcccgtatc cgccagcgcc agggcgacgt cgtcgccgcc
     4561 gataccctgc aggtgggggt cgaggaccag gcagtcgatg tcccggtcgt gctcgaacgc
     4621 gtcgagaaaa gcctctccgc tggcaaagac tttggccagc cagccccgac tctcagcgag
     4681 cagggtcatg gcatccagaa tggccacatc atcgtctacc accgcaatgg aaaccatcgc
     4741 ccaccgtccg catttgcctt ttagcgaggt taaggcgggc agctgtggcc tcaattcggg
     4801 aatctaccta gccgggccca cgtccggcgt cgagtccccg ctcgatgaga ctgatgaggt
     4861 gattggtgtc gacgggtttg cgcaggacgg tgcagttgga tatcgcggcc tcgtcgatga
     4921 ctttgccgga ggtatcaccg gtcatcagca ccgtcggcag atagctgccc cgcagctcgc
     4981 gcgcgcgccg gatgacttcg atgccgttgg cgcccggcag gcggaagtcg gacaccacca
     5041 tgtcgggaga cagcccctcc tccagcatgg cgatggccct gttgccgtcg agcgccgagc
     5101 gcaccgagat cccggcgagt tccagcatca tcgtcgtcgc gtccacgatg gcggggtcat
     5161 cgtctacgaa gaggatcacc ggttcgccag gagccaactg gccggtctgc ggttggcccg
     5221 ggaccgtctc ctcctcgtcc agcagcccca ggggcaccag aatgctgaaa catgagccct
     5281 cgcccacggt ggaagccacc tccagcgggc agtcgagcaa gctggctacc tgtttcacaa
     5341 tgga
//
LOCUS       DBNHLM010000202         3258 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1644976, whole genome shotgun sequence.
ACCESSION   DBNHLM010000202 DBNHLM010000000
VERSION     DBNHLM010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1644976"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..910
                     /locus_tag="AC16QF_09185"
     CDS             131..910
                     /locus_tag="AC16QF_09185"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448773.1"
                     /translation="MNLKGQVAVISGAAVGIGRGIAVALAEQGAAVACLDIDGVGNQE
                     TAGLAEEAGGRGLALDCDVADRAQVRRAMSEALGALGRIDVLVNNAAIYVDTSLTVGS
                     YDAQTRAYERSIAICALGSYYCARAAVPAMRESGGGNIVNIITEHIKEGHLMTGPGAT
                     GYDGAKWVMWRQTESWAVELAEHNIRVNALCMGATDTPMLRAVSVPIAEAGMRPEDVG
                     QAVLNVLAHGRRGPTGQSYVFGTTGTSREQSLGEIAALAPR"
     gene            928..2247
                     /locus_tag="AC16QF_09190"
     CDS             928..2247
                     /locus_tag="AC16QF_09190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6448774.1"
                     /translation="MRDRFSQAYLWYVVAVLTIASTFSIIDRQILNVMIGPVKRDLGG
                     ISDFQVSLIMGFAFTFLYSVLSYPAGWVADRYNRKNLMAVGIAAWSAMTMLCGMVSQY
                     WHLFATRMGVGVGEATLQPAATSSLSDYFPPHMLPLAMGIVASAPFIGQGLANIAGGP
                     LIDYLEATPNYVLPLFGEVFSWQMVFLLVGAPGLLVALAMWGLREPSRHGKQTEEAGV
                     PLREVWAFVKDRGAFFFLVFTAYLCLATQGWSLFSWLVEFYVRNHEWTRTEIGLTYGF
                     IAMFVGMAGSIIGGAWAGRLIRRDSPDGTIRVVMYGSIVLLPVGAAITIVPDPWLGVA
                     LLVPATFCMAMPPGLIMATLQAIAPNELRGQMVAFYLIAVNFLAYTFAPSLPAVLSDF
                     VFGSEMALGKSISLLAVVNYTISSLCLALGLKHYRRALDRARAWASA"
     gene            complement(2297..>3258)
                     /locus_tag="AC16QF_09195"
     CDS             complement(2297..>3258)
                     /locus_tag="AC16QF_09195"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RHO alpha subunit C-terminal catalytic
                     domain-containing protein"
                     /protein_id="HIR6448775.1"
                     /translation="HAQDPEDFDGGSPCGRVSLVEVPCDTWGGFVWYTMDPEAPSLAA
                     FLDPIPELFANRDLDAAVRVVRRAFTVDANWKFASDNFNESYHLPTVHPELATTTDED
                     YRNTIFEAYSTGHNRMIEQGQPSMRAPRPNEVEAPWDEILAAWDLDPADFAARARDGR
                     IALQQQRRKLGPARGYGHYAKYSDDELTDYFHHTLFPNVTLTATADGVHFFRTEPHPA
                     DPEKCTFEYWFFASPVEGQDEVVTICGPRPLQEAEPEQFEYGSGAVIEDMMDSFLIQD
                     LGVAVGQQQGFHSRGYRDAILSGQESRVRRFHEVLNDYLEGRR"
BASE COUNT          515 a         1050 c         1093 g          600 t
ORIGIN      
        1 ggagcacggc cagatccgtc tggtcacctc cggcctgtat cgggacgagg tggtgctgca
       61 caacggtgat tggcgcatcg cccggcggcg gatcgagctg gacatgcctt actgatccca
      121 aggaggctgc gtgaatctca aaggtcaggt tgccgtcatc agtggtgcgg ccgtgggaat
      181 cgggcgcggc atcgccgtgg ctctggcgga acagggcgcg gcggtcgctt gcctggacat
      241 cgacggcgtg ggaaaccagg agacggccgg gctcgcggag gaggcgggtg ggcgcgggct
      301 cgcgctcgac tgtgatgttg ccgatcgagc ccaggtgcgg cgtgccatga gcgaggcgct
      361 gggcgcgctg ggaagaatcg acgtgctggt gaacaacgcc gccatctacg tggacacctc
      421 tctcacggtg ggcagctacg atgcccagac ccgcgcgtac gaacgatcca tcgccatctg
      481 cgccctgggc agctactact gcgcccgggc cgccgtgccc gccatgcgcg agtccggcgg
      541 cggcaacatc gtcaacatca tcaccgagca catcaaggaa gggcacctca tgaccggacc
      601 gggcgccaca ggttacgacg gtgcgaagtg ggtaatgtgg cggcagaccg aatcctgggc
      661 cgtggagctc gccgagcaca acatacgggt caacgcgctc tgcatgggcg ctaccgacac
      721 gccgatgctg cgggcggttt cggtgcccat cgccgaggcc ggcatgcggc cggaggacgt
      781 gggccaggcg gttctgaacg tgcttgccca cggtcggcgc ggaccgacgg ggcaatccta
      841 cgtcttcggc accacgggca cgtcccggga gcagagtctc ggcgagatcg ccgctctggc
      901 tccgcgctga gcccgccccg ggcacccatg cgggacaggt tcagccaggc atacctttgg
      961 tacgtggtgg cggtgctgac catcgcctcc accttctcca tcatcgatcg tcagatcctg
     1021 aacgtgatga tcggacccgt gaagcgcgac ctgggcggca tctcggattt ccaggtctcg
     1081 ctcatcatgg gcttcgcctt caccttcctc tacagcgtgc tttcctatcc ggccggctgg
     1141 gttgccgacc gctacaaccg gaagaacctg atggccgtgg gcattgccgc ctggagcgcg
     1201 atgaccatgc tttgcggcat ggtgagccag tactggcacc tcttcgccac gcgcatgggg
     1261 gtcggggtcg gcgaggcgac tctgcaaccg gcggccacgt cctcgctttc cgactacttt
     1321 ccacctcaca tgctgccgct cgccatgggc atcgtggcct cggcgccatt catcgggcag
     1381 ggcctggcca acatcgccgg cgggccgctt atcgactacc tggaggcaac acccaactac
     1441 gtgctgccgc tctttggtga ggtattttcc tggcagatgg tgttcctcct ggttggcgcg
     1501 cccgggctgc tggtggcgct cgccatgtgg gggctgcgcg agccgtcccg acacggcaag
     1561 cagactgaag aggccggcgt gccgctccgc gaggtctggg ccttcgtcaa ggatcggggg
     1621 gctttcttct tcctggtctt caccgcctac ctgtgcctgg ctacccaggg ctggtcactt
     1681 ttctcctggc tcgtggagtt ctacgtgcgc aaccacgagt ggacgcgcac ggagatcgga
     1741 ctgacctacg ggttcatcgc catgttcgtc ggcatggcgg gcagcatcat cggcggggcc
     1801 tgggcggggc ggctgatccg ccgcgactcg ccggacggca cgatccgggt cgtcatgtat
     1861 ggatccatcg tgctcctgcc agtcggggcc gccatcacca tcgtccccga cccctggctg
     1921 ggcgtggctc tgctggtgcc cgccacgttc tgcatggcca tgccgccggg cctcatcatg
     1981 gcgaccctgc aggccatcgc tcccaacgag ctgcgcggcc agatggtggc cttctatctc
     2041 atcgcggtga actttctcgc gtacacgttc gcgccgtcat tgccggccgt gctgagcgat
     2101 ttcgtgttcg gcagcgagat ggcgctgggt aagtccatct cgctgcttgc cgtcgtgaac
     2161 tacacgatct ccagcctctg cctggccctg ggtctgaagc actatcgacg cgctttagac
     2221 cgcgcgcggg cgtgggcgag cgcctagcgc tccgacgaca tctcaagcag gccgatttcc
     2281 acgcagctgg cggatgctaa cggcgacctt ccagatagtc gtttaaaacc tcgtggaagc
     2341 gccgcacccg gctctcctga ccgcttaaga tcgcgtcgcg atagccgcgg gaatgaaacc
     2401 cctgctgctg gccgaccgcc accccgagat cctggatcag aaacgaatcc atcatgtcct
     2461 cgatgaccgc accggagccg tattcgaact gttcgggctc ggcctcctgc agtggacggg
     2521 ggccgcagat cgtgaccact tcgtcctgtc cctcgacggg cgatgcgaaa aaccagtatt
     2581 cgaacgtgca tttctcagga tcggcgggat gaggctccgt acgaaaaaag tgcacgccgt
     2641 ccgccgttgc cgtgagcgtc acgttgggga acagggtgtg atgaaaatag tcggtgagct
     2701 cgtcgtccga gtacttcgcg tagtgtccgt agccgcgcgc ggggccgagc ttgcgccgtt
     2761 gctgctggag cgcgatgcgc ccatccctgg cacgcgcggc aaaatcggcg ggatccaggt
     2821 cccacgcggc caggatctca tcccacgggg cctccacctc gttggggcgt ggcgcgcgca
     2881 tggaaggctg gccctgctcg atcatccggt tgtggccggt ggagtaggct tcgaagatcg
     2941 tgttgcggta gtcctcgtcc gtcgtcgtcg cgagctcggg gtgcaccgtg ggcaggtggt
     3001 aggactcgtt gaagttgtcg gaggcaaatt tccagttggc gtccacggtg aaggcgcgcc
     3061 gcaccacgcg aacggcggcg tcgagatcgc gattggcgaa cagctccggg atggggtcta
     3121 aaaacgccgc gagtgagggc gcctccggat ccatggtgta ccagacgaac ccgccccagg
     3181 tgtcgcaggg cacctcgacc agggagacgc ggccgcacgg gctgccgccg tcgaagtctt
     3241 cggggtcctg cgcgtgca
//
LOCUS       DBNHLM010000203         4802 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_387764, whole genome shotgun sequence.
ACCESSION   DBNHLM010000203 DBNHLM010000000
VERSION     DBNHLM010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_387764"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="AC16QF_09200"
     CDS             <1..1091
                     /locus_tag="AC16QF_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HIR6448776.1"
                     /translation="PPPPRYDGERVGPERAAERIGVDDAFPVNDLEEILPGMLEGRER
                     IYITLGEYPEFDNRLMHWVAGIRAREAGGAIPPGEFVALKHLLHEQRLYKSAAELRAM
                     REAARITCSAHRRAMAACRPGMTETQLEAELLHEFMVNGARSPAYPSIVGAGANACVL
                     HYVDNHDVLKKGDLVLIDAGCEYQHYASDVTRTFPVAGKFSAAQRALYEVVLEANRQG
                     IEACRPGATFIEPHSTALKVMVEGLIELNLIQGDVEEILQSERYREFCPHNSSHWLGS
                     DVHDVGDYRVEGAWRPLEPGMVLTVEPGIYIPADDSTRHLAPRWRGVGIRVEDDVAIT
                     RQGHEVLSASAPKSVDDIERLMRGGADG"
     gene            1084..2265
                     /locus_tag="AC16QF_09205"
     CDS             1084..2265
                     /locus_tag="AC16QF_09205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6448777.1"
                     /translation="MADVVVVGGGLIGALAALGVADGGRRVTVLERREPERQRGRLGV
                     DIRNVAVSPASRELLEAVGVWSSLEPAAYQRMEVWEERGTRGMHFDAAEVGRAELGWI
                     LESSQVVRSLWDLLQRHRNVRLEVGADLASLRLAASGAVLETDRGDIRARLVVAADGA
                     RSRVRELLAVPAEEAPTGHHALATVVRTEQEHEGTCLQRFLLEGPLALLPTADAHLSS
                     VVWSQPAEEAERRAKEPDGDFCLELERCLEARFGKVEAVDARVVFPLAQTLVSTFNPH
                     HRVLLIGDAARVVHPLAGMGANIGFEDVRDLLAELAGIGGDQDPGAPGIWRRFARQRR
                     LRSQMVLALMSGFRRAYSADGPLASWVRNTGVGWLDRAQPLKAQIMREALGVGPLARR
                     W"
     gene            2354..3421
                     /gene="gcvT"
                     /locus_tag="AC16QF_09210"
     CDS             2354..3421
                     /gene="gcvT"
                     /locus_tag="AC16QF_09210"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR6448778.1"
                     /translation="MTRRTPLYELHVGLGAKMVDFAGWEMPLHYGSQLDEHHAVRRHV
                     GLFDVSHMTIVDVSGARSLDFLQRLVANDVARLEAPGQALYGVLLNPAGGVIDDLIVY
                     RRDADFRLVVNAATGEKVLNWLAGNNEEAVNILERDLAMLAIQGPAAVEMYQSLPDSL
                     EVRGLKAFTAAVRGEEMIARTGYTGEDGLEIMAPGDRARQLWLELIGSGAQPAGLGAR
                     DTLRLEAGLNLYGQDMDDETSPLVSNLAWTIAWEPSGRNFIGRQALEDERARGIAERL
                     TGIVLEDKGVIRRGCRVVTAAGDGHVTSGIFSPTLGYSIGLARVPKGAKGPCEVEMRG
                     RLKQARIVRPPFVRNGLQVFE"
     gene            3418..3813
                     /gene="gcvH"
                     /locus_tag="AC16QF_09215"
     CDS             3418..3813
                     /gene="gcvH"
                     /locus_tag="AC16QF_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR6448779.1"
                     /translation="MSDVPQELKYARTHEWARLEEDGTITVGISDHAQDALGDVVYVE
                     HPEPGQPVAAGDEAGVVESVKAASDIYAPVSGSVIAVNEALDDAPETVNQDPYGDGWF
                     FKIQPDDVAELDELLDSEAYAELCENEEH"
     gene            3845..>4802
                     /gene="gcvPA"
                     /locus_tag="AC16QF_09220"
     CDS             3845..>4802
                     /gene="gcvPA"
                     /locus_tag="AC16QF_09220"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HIR6448780.1"
                     /translation="MPFIPHTAEEVQVMLSQIGVARTEDLFDEIPQNLRAGALSSVPA
                     GASELEMLRKLGARAALDESVVCFAGAGSYDHHIPAAVWDLTSRGEFMTAYTPYQAEA
                     SQGTLQLIYEYQSMMTALTGLDVSNASVYDGASGLAEAVLMAVRSNKKARSRTVLLPD
                     SLHPHYAAAARNICRHQSVEFVGMALDHDGRVDLEALETDGPDEVCAVVVQQPNFFGM
                     LEDVDALTDWAAERGGLVIAVVNPLALAVLKPPGEWGGSGADIACGEGQPLGVPMSSG
                     GPAFGFICCRSALVRQLPGRVIGRTEDLTGKPGFTLTLQAREQ"
BASE COUNT          786 a         1483 c         1722 g          811 t
ORIGIN      
        1 cccccccccc cccccgctac gacggcgagc gggtagggcc cgagcgcgcc gcggagcgca
       61 ttggcgtgga cgacgctttc cccgtcaacg acctggagga gattctgccc ggcatgctcg
      121 agggacggga gcggatctac atcaccctgg gcgaataccc ggagttcgac aaccgcctga
      181 tgcactgggt cgccggcatc cgcgcgcggg aagcgggcgg ggccattccg ccgggtgagt
      241 tcgtcgcgct caagcatctg ctgcacgagc agcgtctcta caaatcggcc gccgagctca
      301 gggcgatgcg ggaggcggcc cgtatcacct gctccgccca tcgccgcgcc atggcggcct
      361 gtcggcccgg aatgaccgag acgcagctgg aagccgagct gctgcacgag ttcatggtca
      421 acggtgctcg cagcccggcc tatccgagca tcgtgggggc cggcgcgaac gcctgcgttc
      481 tgcactacgt ggacaaccac gacgtgctga agaagggcga tctcgttctc atcgacgccg
      541 gctgcgagta tcagcactac gcctcggacg taacccgcac gtttcccgtt gccggtaagt
      601 tttctgctgc ccagcgggct ctctacgagg tcgtcctcga agccaatcgt cagggcatcg
      661 aggcctgtcg ccccggtgcg acgttcatcg agcctcactc tacggcgctc aaggtcatgg
      721 tggaggggct catcgagctg aatctcatac aaggcgatgt ggaggagatt ctccagagcg
      781 aacgctatcg tgaattctgt ccccacaact cgtctcactg gctgggcagc gacgtccacg
      841 acgtgggcga ctaccgggtc gaaggtgcct ggcggccgct ggagccgggc atggtgctca
      901 ccgtggagcc cggcatctac atcccggccg acgactctac ccgacacttg gcgccgcggt
      961 ggcgcggcgt gggcattcgt gtggaagacg acgtggccat caccaggcag ggccatgaag
     1021 tgctttcggc gtcggccccc aagtcggtgg acgacatcga gcggctgatg cgtgggggcg
     1081 ccgatggctg acgtggtcgt ggtgggcggt gggctgatcg gagcgctggc cgccctcggc
     1141 gtcgccgatg ggggacgacg ggtcacggtg ctcgaacggc gcgagccgga aaggcagcgg
     1201 ggcaggctgg gcgtcgacat ccgcaacgtg gccgtatcgc cagcgtcccg cgagcttctg
     1261 gaagccgtcg gtgtgtggtc atcgctcgag ccggctgctt accaacgcat ggaggtatgg
     1321 gaagagcgag gcactcgcgg catgcacttc gacgcggcag aggtaggccg ggccgagctc
     1381 ggctggatcc tggaatcgag ccaggtcgtg cgctcgctct gggatctgct gcagcggcac
     1441 cggaacgtcc ggctggaggt cggcgcggat ctggcgagct tgcgcctggc ggcttcgggc
     1501 gccgtcttgg aaaccgaccg cggagatatc cgggcgcgcc tggtggtggc cgctgacggt
     1561 gcacgttcca gggtgcggga gctgctcgcg gtgcccgccg aagaagctcc caccggtcat
     1621 catgccctcg ccacggtggt gcgcaccgag caagagcatg agggcacctg cctgcagcga
     1681 ttcctgctcg agggcccgct tgcgctgctg ccgacggcgg acgcccacct ttcatccgtg
     1741 gtctggtcgc agcccgcgga ggaggccgag cggcgtgcga aagagcccga cggcgacttc
     1801 tgcctcgagc tggagcggtg tctggaagca cgatttggaa aggtggaagc ggtggatgcc
     1861 cgggtcgtgt ttcccctggc gcagacgctg gtgtcgacct tcaatcccca ccacagggtg
     1921 ctgctgattg gcgacgccgc cagggtcgtg caccccctcg cgggcatggg cgcgaacatc
     1981 ggcttcgagg acgtgcgcga tctgctggcc gaactcgccg ggataggcgg cgatcaggac
     2041 ccgggagcgc cgggaatctg gcggcgcttt gcgcggcagc gacggctacg atcccagatg
     2101 gtgctggcgc tgatgtccgg cttccggcgc gcctattctg cggacggccc gttggccagc
     2161 tgggttcgga acacgggcgt tggatggctc gaccgtgcac agcctttgaa agcacagatc
     2221 atgagagagg ctttgggggt cgggccgctc gccagaaggt ggtgacgccc accgtttccg
     2281 gggccacccg gttcgcgtca tcccatgcgt catcgcatac aatgtggtgc gtttttccaa
     2341 tggtggtctg agcatgacgc ggcgaacccc gctatatgag ctgcatgtcg ggctgggcgc
     2401 gaagatggtc gacttcgccg gctgggaaat gccgctccat tacggatccc agctggatga
     2461 gcatcacgcc gtgcgtcggc acgttggcct gttcgacgtc tcccacatga ccatcgtgga
     2521 tgtcagcggt gcccgcagtc tcgacttcct ccagcggctg gtggccaacg acgttgccag
     2581 gctggaagcg cccggtcagg cgctgtacgg ggtgctgctc aatccggcag gcggggtgat
     2641 cgacgacctc atcgtctatc gccgcgatgc cgacttccgg ctagtggtca atgccgccac
     2701 tggagaaaag gtgctcaact ggctcgcggg caacaacgag gaagcggtca acattctgga
     2761 gcgggacctg gccatgctgg ccatccaggg ccccgcggcc gtggagatgt atcagtccct
     2821 gcctgacagc ctcgaggtcc ggggcttgaa ggcgtttacc gcggccgtgc gcggggagga
     2881 gatgattgcc cgaacggggt acacggggga ggacggtctg gagatcatgg ccccgggcga
     2941 tcgcgcccgt cagctttggc ttgagctcat cgggtccggg gctcagccag cggggcttgg
     3001 ggctcgcgac actctgcgcc tggaggctgg cctgaacctc tacggccagg acatggacga
     3061 tgaaacctcg cccctggtgt ccaatctggc ctggaccatc gcctgggagc catccggacg
     3121 gaacttcatc ggccgccagg ccctcgagga cgaacgcgca cgcgggatcg cggagaggct
     3181 caccggtatc gtgctcgagg acaagggggt gattcggcgg gggtgccgcg tagtgaccgc
     3241 ggccggtgat gggcacgtca ccagtggtat attctccccg acgttgggat acagtattgg
     3301 gctcgcgcgg gtgccgaaag gcgcgaaggg gccgtgcgag gtagaaatgc ggggaaggct
     3361 gaaacaggcg cgaatcgtcc ggcctccctt tgtgcgtaat ggtttgcagg tattcgaatg
     3421 agcgacgttc cacaggagct taaatatgcg cgcacccacg agtgggcgcg gctggaggag
     3481 gacggcacca tcaccgtcgg gatttccgac cacgcccagg atgcgcttgg cgatgtggtc
     3541 tacgtggagc atccggagcc gggtcagccg gttgcggccg gcgacgaggc gggcgtcgtc
     3601 gagtcggtga aggcggcctc cgacatctac gcgccggtaa gcgggagcgt cattgccgtg
     3661 aacgaggcgc tggacgacgc cccggagacc gtgaaccagg atccctacgg tgacggctgg
     3721 ttcttcaaga tacagccgga cgacgttgcc gagctggacg agctgctcga ttcggaagcc
     3781 tacgccgaac tatgcgagaa cgaagagcac tgagtccttg atacccatct aaacggagcc
     3841 cccgatgccc ttcataccgc acaccgcgga agaagtgcag gtcatgctgt cccagatcgg
     3901 cgtggcccgc acggaagacc tgttcgacga gattcctcag aatttgagag caggcgcgct
     3961 gagctcggtc cccgcggggg ccagcgagct ggagatgctg cgcaagctcg gcgcgcgcgc
     4021 ggcgctcgac gagtcggtag tctgcttcgc cggggcgggc agttacgacc atcacattcc
     4081 cgcggcggtc tgggatctga cgagccgcgg cgagttcatg acggcgtaca cgccctatca
     4141 ggcggaagcg agccagggca cgctgcagct gatctacgag taccagtcga tgatgacggc
     4201 gctcacgggt ctggacgtct ccaacgcgtc ggtttacgac ggcgcgtcgg gcctggcgga
     4261 ggccgtgctc atggcggtgc gaagcaacaa gaaggcccgg tcgcgaaccg ttctgctgcc
     4321 ggactccctg catccgcact acgcggccgc ggcccgcaac atctgccggc atcagagcgt
     4381 ggagttcgtc ggcatggccc tggaccacga cggccgcgtg gatctcgagg ccctggagac
     4441 cgatggtccg gacgaggtct gcgcggtggt ggtgcaacag ccgaacttct tcgggatgct
     4501 cgaggacgtc gacgccttaa cggattgggc ggccgagcgg gggggcctcg tgatcgcggt
     4561 ggtcaacccg ctggctctgg ctgtgctgaa gcctcccggt gagtgggggg gcagcggggc
     4621 cgacatcgcc tgcggcgaag gccagccgct cggcgtgccc atgtcttcgg gggggccggc
     4681 gttcggcttc atctgttgcc gctcggccct ggtgcgtcag cttccgggaa gggtgatcgg
     4741 tcggacggag gacctgacgg ggaagccggg cttcactttg accctgcagg ccagggagca
     4801 gc
//
LOCUS       DBNHLM010000204         6360 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_29676, whole genome shotgun sequence.
ACCESSION   DBNHLM010000204 DBNHLM010000000
VERSION     DBNHLM010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_29676"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="AC16QF_09225"
     CDS             <1..543
                     /locus_tag="AC16QF_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448781.1"
                     /translation="LPDVEELDWDEESVVPTRAKVVDDLDTDFIPPGQYYVESDTGEI
                     LRNWKIGYQQIDTDGTQAVHGWIGRRKHELRHATFEILTPKAAVAVSSLDSQAIPQSS
                     RILITAIGRAVVRKESGKEHIMSEPIVGKLRVSAASGMRLFPLDERGNHLDPIEPAYR
                     DGAYEILLPVPGGTHWYLLK"
     gene            1020..1826
                     /locus_tag="AC16QF_09230"
     CDS             1020..1826
                     /locus_tag="AC16QF_09230"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6448782.1"
                     /translation="MKYKALAFDLDGTLLIGEALPEPNRLAVRAAFEAGYRIIIATAR
                     WRQMAQRIEAQIGISGPAITCSGAQVYCPDIGGDVFDERLPADFTNDLYAICDAERCI
                     ATVTVDDRVLLKLDGKPEPGLFPEEMEHVAQLAGADPSLPRVAAIQGSHVNRLIREEL
                     QPIYQDRVHFFDSIGPTGKVILTLTASTASKGDALRAACEHLGIDAAEVIAFGDSEND
                     IEMFRAAGASVAMGQADERTRAAATVVSAPHDEDGVANAVEHLLRTGDIV"
     gene            1828..2754
                     /locus_tag="AC16QF_09235"
     CDS             1828..2754
                     /locus_tag="AC16QF_09235"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6448783.1"
                     /translation="MQPGKLTVSDGAEIHYYRWRPGGEIRGAVQIAHGMGEHAGRYDA
                     LAGRLNQEGYAVYANDHRGHGLTAGADRLGIFGRGGWDRVIQDARELNDHVASKHPSA
                     PRALLGHSMGAMLTQQYIARHGDTIHAAVISGSPGFGSAFGLWLSQTMARLEHWRHGD
                     VVTERMQERVFAAPNASFEESEATGFEWLSRDREEVRKYVEDPYCGFVVSAGSLSALL
                     GGAREARRRRTIGNIPAHLPVYVFSGSDDPVHGNERGLRNLLEGYRSRLHRVDYRLYT
                     EGRHEMLNEVNRHEVVDDLIAWLNERMGPAQS"
     gene            complement(2745..3743)
                     /locus_tag="AC16QF_09240"
     CDS             complement(2745..3743)
                     /locus_tag="AC16QF_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR6448784.1"
                     /translation="MPRYVMLMRYHSAGMRAIESNPQTLYEIHDAIARWEAKVLESYR
                     LLGEWDQCTIFEAPDNFKAYRATLAQELGTTADTEILPAIDFDLFSRLVSTEAGSVGP
                     HRWQITWWARIGRRLFRWHAYDRFANRYCKPLTVTGHHKFDTIKGPCIIVANHTSHMD
                     ALVLNASLPNRIRWNVYSGAAADRWFVKGRKELVMQPWYQSLAMGTFPIQRGGGSKAL
                     DYPKWLLENGCNLIIFPEGTRSTSRSMAKFRHGVSVLALEKNVPVVPVFLTGLKAMRP
                     KGSREIFPGPAGANVLDPIYFDEGTTVPDATRRIYDALNEVHLKVGEFGDEAARQD"
     gene            complement(3747..4052)
                     /locus_tag="AC16QF_09245"
     CDS             complement(3747..4052)
                     /locus_tag="AC16QF_09245"
                     /inference="COORDINATES: protein motif:HMM:NF020317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR6448785.1"
                     /translation="MATYIMLSTLTDEGRKTLKQRPERLKEVNKEVESMGARVVSQYA
                     VLGGYDFVNIIEAPTNEVMSRISMELGARGTIKVTTMPGMTIESFVDMLEGKTTSGN"
     gene            4266..4661
                     /locus_tag="AC16QF_09250"
                     /pseudo
     CDS             4266..4661
                     /locus_tag="AC16QF_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
     gene            4713..5135
                     /locus_tag="AC16QF_09255"
                     /pseudo
     CDS             4713..5135
                     /locus_tag="AC16QF_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
     gene            complement(5140..>6360)
                     /locus_tag="AC16QF_09260"
     CDS             complement(5140..>6360)
                     /locus_tag="AC16QF_09260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHA/PHB synthase family protein"
                     /protein_id="HIR6448786.1"
                     /translation="SLTRGLKNFLDDLRHNHGYPAVADRHAFKVGKDVACTRGAVVYR
                     NELFELIQYRPTTPEVSPVPMLYVFSQVNRFYLGDLTPDRSLFKRLLDAGIPVFAISW
                     RNPTVEERDWNLDTYADGVITAITVMQQITKQPKIHLMGLCAGGLVAAAAAGVLAARR
                     EDWISTLSLFVNVLDYRPGDSEFGLFVSERSVDAQKAVVRGRGVFAERDVFEMFALLR
                     MEENVMSFFRSNYLMGEEPLKHPLLFWSMDYTRVPAEMQCDFLNLAQYNKLAKKEMRI
                     LGKRVDLTRIDYPVYVMAGTTDHITPWKACFRSTQLFGGDVAFVLTNQNHTQTISSRE
                     DNRHLKHWVVDNPSDNPDRAMRLATEHEGSWTRHWIGWLDDHSGERVKAPRRMGSGKN
                     PILGEAPGLYVLES"
BASE COUNT         1234 a         1993 c         2003 g         1130 t
ORIGIN      
        1 cttcccgatg tcgaagaact ggattgggat gaggaaagcg tagtccctac gcgcgcgaag
       61 gtggttgatg atctcgacac tgacttcatc ccgcccggtc agtattacgt ggagtcggat
      121 accggggaaa ttctgcgaaa ctggaagatc gggtatcagc agattgatac cgacggcaca
      181 caagccgttc acgggtggat aggccgcagg aagcatgaac ttcgccatgc aacatttgag
      241 attctgacac ccaaagccgc cgtggcggtt tcaagcctgg acagccaggc aattccacaa
      301 tcaagccgca tactcatcac tgcgatcggc cgggctgtag tgcgcaagga atcgggcaag
      361 gaacacatca tgtcagagcc catcgtgggg aaattacgag tcagcgccgc gtccggaatg
      421 cgcttgttcc cgctcgacga gcgaggcaat catctcgatc cgatcgaacc ggcctaccga
      481 gacggcgcgt acgaaatcct gctccccgtt ccgggaggaa cccactggta tcttctgaag
      541 tgaaacgggc tcggagttgc aaaggggcca cggctcgctc ccaataactt gagatagacc
      601 gagaccaggc acggcaccgt gcatttgttc gcacgtgccg gtatcctccg acctgcccgc
      661 ccgggtggtg aaactggtag acacaaggga cttgagcgcg attgagcacc cggcgtcagg
      721 aaacggccgg cgtgaatgga gtcaaattcg gggaaacctc agcacggcgc tgacaaggcg
      781 cctggtggtg gcaatcccga gctaaggacc agcctgaacg ggccggtcaa agtgtagaga
      841 cttgacggct cctgcctaac tcaccaagcg gtgacatggc aaagagaaag tccagaccac
      901 aaagcgccgc gcacgcggcg tggcagcgaa agctgtagct ggtaagaaaa tccctcggag
      961 gcaactccgt gccggttcga ttccggcccc gggcaccaat caaagacgac ggaacagcaa
     1021 tgaagtacaa ggcattggca ttcgacctgg atggcaccct gctcatcggc gaggcgctgc
     1081 ccgagccgaa ccggcttgcc gtgcgcgccg cttttgaagc cggctaccgc atcatcatcg
     1141 cgaccgcccg ctggcggcag atggcacagc ggatcgaagc acagatcggc atcagcggcc
     1201 cggccatcac ctgctccgga gcccaggtct actgcccgga cattggcggg gacgtgttcg
     1261 acgagcgcct gcccgccgac tttaccaatg atctttacgc gatctgcgac gccgagcgct
     1321 gcattgccac cgtcacggta gacgaccggg tactgctcaa gctggacggc aaacccgaac
     1381 ccggcctctt ccctgaggaa atggagcatg tcgctcagct ggcaggagcg gatccctcac
     1441 ttccccgggt ggccgccata caaggctccc atgtgaaccg cctgattcgc gaggagctac
     1501 agccgattta tcaggatcgc gtgcacttct tcgacagcat cggccccacg ggcaaggtga
     1561 tcctcacgct cacggcaagc acggcgagca agggcgatgc cctgcgcgcg gcctgcgaac
     1621 acctcggaat cgatgctgcc gaggtaattg ccttcggcga ttcggagaac gacatcgaga
     1681 tgtttcgggc cgccggcgcg tccgtcgcca tgggacaggc cgacgagcgc accagggcgg
     1741 cggccaccgt ggtcagcgcc ccccatgacg aggacggggt cgccaacgcc gtggaacacc
     1801 tgctgcgcac gggagacatc gtctgacatg caaccgggca agctcaccgt cagcgacggc
     1861 gccgagatcc actactaccg ctggcgtccc ggcggcgaga ttcgaggcgc ggttcagatc
     1921 gcccacggca tgggcgagca tgccggccgc tacgacgcgc tggccggccg cctcaatcag
     1981 gaagggtacg cggtttatgc caacgaccac cgcggccacg ggctcacagc aggtgcggac
     2041 cggttgggca tcttcggtcg gggcggctgg gaccgggtca ttcaggatgc cagggaactc
     2101 aacgaccacg tcgcatccaa gcatccgagc gcgcctcggg cactgctcgg tcacagcatg
     2161 ggcgccatgc tcacccagca gtacatcgcc cgccatggcg acacgatcca cgcggccgtc
     2221 atcagcggct cccccgggtt cggttcagcc ttcggcctct ggctttctca aaccatggcc
     2281 cgcctcgagc actggcggca tggcgacgtg gtcaccgagc gaatgcagga acgggtgttc
     2341 gccgcaccca atgcgtcgtt tgaggaatcc gaggcaacgg gtttcgagtg gctttccaga
     2401 gaccgggagg aagtccggaa gtacgtggag gacccgtatt gtgggttcgt ggtgagtgcc
     2461 gggagtttgt ccgccctgct agggggcgca cgggaagccc ggcgcaggcg caccatcggc
     2521 aacatccccg cccatcttcc cgtctacgtg ttctcaggca gcgacgatcc ggttcacgga
     2581 aacgagcgcg ggctgcgcaa cctgctcgag gggtaccgct cccgcctcca ccgggtcgac
     2641 taccgcctct acaccgaggg gcgccacgag atgctcaacg aggtcaaccg gcacgaggtg
     2701 gtcgacgacc tgatcgcctg gctgaacgaa cgcatgggcc ccgctcagtc ctgacgcgcc
     2761 gcctcgtcgc cgaactctcc aaccttcaga tggacctcgt tgagggcgtc gtaaatgcgc
     2821 cgggtggcat ccggaaccgt ggtcccttcg tcgaagtaga tgggatcgag cacgttggcg
     2881 cctgcaggac cgggaaaaat ctcgcgggag cccttgggcc gcatggcttt gaggccggtg
     2941 agaaaaaccg ggacgacggg cacgtttttc tccagcgcca gaacggacac cccgtggcga
     3001 aacttggcca tgctccggct ggtggagcgc gtgccctcag gaaagatgat gaggttacag
     3061 ccgttttcga gcagccactt gggatagtcc agcgccttcg aaccgccgcc tcgctggatc
     3121 ggaaacgtgc ccatggccag cgactggtac cagggctgca tcaccagctc cttccggcct
     3181 ttcacgaacc agcgatcggc cgccgctccg gagtagacgt tccagcgaat acgatttggc
     3241 agtgaggcgt tgagtaccag cgcgtccatg tggctcgtat ggttcgccac gatgatgcac
     3301 ggacctttga tggtgtcgaa cttgtgatgg ccggtgaccg tcaggggttt gcagtagcga
     3361 ttggcaaaac ggtcgtaggc atgccagcgg aagaggcgtc ggccgatcct ggcccaccag
     3421 gtgatctgcc aacgatgggg cccgacgctg ccggcttccg tgctgaccag ccgggaaaag
     3481 aggtcgaagt cgatggcggg cagaatttcc gtatctgccg tggtgccgag ttcctgagca
     3541 agggtggccc gatacgcctt aaagttgtcg ggcgcctcga aaatcgtgca ctgatcccac
     3601 tcaccaagaa gccgatagct ttccagcacc ttggcttccc agcgcgcgat ggcgtcgtga
     3661 atctcgtaca gggtctgggg gttcgactcg attgctcgca tgccggccga gtgataacgc
     3721 atgagcatca catatcgagg catgtttcag ttgccgctgg tggtctttcc ctcgagcatg
     3781 tcgacaaacg actcgatcgt catgccgggc atcgtggtca ccttgatggt gcctcgtgct
     3841 ccaagctcca tggagatgcg cgacatgacc tcgttggtgg gggcctcgat gatgttgacg
     3901 aaatcgtatc ccccgagcac ggcatactgc gaaaccaccc gggcccccat ggattccact
     3961 tccttgttca cctctttcag gcgctcgggg cgctgcttca aggtcttgcg cccttcgtcg
     4021 gtcagcgtgc tgagcatgat gtaagtcgcc acgatgattc cccgctattg ctgcgaatag
     4081 catacgcctt taccggtcac gcgccagcgt tgtgcctttg ccgacgggcg tttagcatgg
     4141 tttgaggttt cagcagtccc ccggccattg gccgaggccg ccagggctga agcctgattc
     4201 gccacgggaa cgggttctcg tgaaacatgc tgggggcaga tggaaacatg agctttacca
     4261 aagtaatggc cgccgtcgac tgggacgaag cgagcgccgc ggtagtgatc gagaccgccc
     4321 ggagagcagc cggcggccgg ccggtggaga tcgttcacgt gctggatcag gggtattacc
     4381 tcaacaccat cgatcccgcc tccgcctcca tcgaagatct ccgcgccaag gcctcctccg
     4441 aggccgaggc ttatctgcag ggtctctgcg accggcatgg ggggacgtat gccctgctcg
     4501 aggggcatgc ggcaacgatg ctgcacgaac acgccgagag cggcgggcat gacctgctgg
     4561 tggtcggaag tcacggccgc cacggactca agctgcttct gggatccacc gccaacgccg
     4621 tgctgcatgg caccaaccag cacgttctgg ccgtccgaat tcccggacgc ggcgaggaag
     4681 ccgaggagtt ttcccacgcc taccgtcgat tgatcgcctg cgtcgatctg accgacgagt
     4741 caagccaggt tctcgacatt gcgcagacac tggcgtcaga acacggtgcg gagatgcacg
     4801 tggcccacgt gatcaagccg ctgcagcagg cctacgccgg ggtcaaccca tcgacgatgt
     4861 ccgacgtggg aattcagttc gagcacgaag ccgacgccca ggcccgggac gagctcgcga
     4921 gaattgccga tgtccacggg ctggcggccg accggctgca cgtgcggcac ggcgccccgg
     4981 ctgcggagat ccacgctctc gtcaaagaac ttggggcgga tctggtggtg atcggaaccc
     5041 acggcaagca cggcgtgcaa ctgctgcttg ggtccaccgc caacgcgacc cttcacggcg
     5101 caacctgcga caccctggcc gtgaggatcc gctgaggctt caggactcca gcacgtacag
     5161 accgggggcc tcccccaaaa tggggttctt gcccgagccc atgcgccgtg gcgctttgac
     5221 cctttccccg ctgtgatcgt cgagccagcc gatccagtgt ctcgtccatg acccctcgtg
     5281 ctcggtggca agccgcatgg cccgatccgg attatccgac gggttgtcga cgacccagtg
     5341 cttgaggtgt cggttgtcct cccggctgga gatcgtctgg gtgtggttct ggttggtcag
     5401 gacgaacgcc acgtcgccac caaacagctg ggtgctccgg aaacaggctt tccagggcgt
     5461 gatgtggtcc gtggttccgg ccatgacgta gaccggatag tcgatcctgg tgaggtcgac
     5521 ccgcttgccg aggatgcgca tctccttctt ggcgagcttg ttgtactggg ccaggttcag
     5581 gaaatcgcac tgcatctccg ccggcacgcg ggtgtagtcc atggaccaga acagcagcgg
     5641 atgctttaag ggctcctccc ccatgagata gttgctgcgg aagaagctca tcacgttctc
     5701 ttccatgcgc agcagggcaa acatctcgaa cacgtcccgc tccgcgaaga cgccgcgccc
     5761 gcggacgacg gctttctgag cgtccaccga acgctcgctg acgaacagcc cgaactcgct
     5821 gtcgcccggc cggtaatcga gcacgttgac gaacagggaa agggtgctga tccagtcctc
     5881 gcggcgcgcc gccaataccc cggccgccgc tgcggccacc aatcctccgg cacacagacc
     5941 catgaggtgg atcttgggct gcttcgtgat ctgctgcatg acggtgatgg ccgtgatcac
     6001 gccgtcagca taggtatcga ggttccagtc ccgctcctct acggtggggt tgcgccagct
     6061 gatggcgaat accggaatgc cggcatcgag cagccgcttg aagaggctgc gatccggagt
     6121 cagatcaccc aggtagaagc ggttcacctg gctgaacacg tacagcatag gcaccggcga
     6181 gacctcaggg gtggttggcc gatactggat cagctcgaag agctcgttcc gatacaccac
     6241 cgcgcccctg gtgcaggcca catccttgcc gaccttgaag gcgtgccggt cggctaccgc
     6301 ggggtagccg tggttgtgcc ggagatcgtc cagaaagttt ttcagcccgc gagtgaggct
//
LOCUS       DBNHLM010000205        16705 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1107248, whole genome shotgun sequence.
ACCESSION   DBNHLM010000205 DBNHLM010000000
VERSION     DBNHLM010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 16705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16705
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1107248"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /gene="secF"
                     /locus_tag="AC16QF_09265"
     CDS             complement(<1..830)
                     /gene="secF"
                     /locus_tag="AC16QF_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR6448787.1"
                     /translation="MKPLNLNIDFMGKRRIAGGISMVLVLVSLIAVFSVGLNLGLDFT
                     GGTLVEVEFVEPTNPNTVRELLENDGYSNGVVQYFGTERDLLIRMPPQEDGEQANMGD
                     EILAVLSARYPGVVMRQSNYVGPAVGEELTEDGGLAVLAALFVVMLYILFRFTKQFSV
                     GAVVALIHDVAIVLGIFAMAQWTFDLPVLAALLAVIGYSLNDTIVVSDRIRENFRKIR
                     KGAAVDIINTSLNQTLGRTLVTSLTTLLVLLALLFAGGEMIRGFALALTIGVLVGTYS
                     S"
     gene            complement(827..2701)
                     /gene="secD"
                     /locus_tag="AC16QF_09270"
     CDS             complement(827..2701)
                     /gene="secD"
                     /locus_tag="AC16QF_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR6448788.1"
                     /translation="MSKARRRAPNTHSVGRYLFILFVVVIGGVYAAPNLFQPDPALQI
                     KSTNEANPLNQEVLERVTAALAARGVTVKGTELAGDSALVRVGTDEQQLRGRDIATAE
                     LNPESSGRYVVALNRASTTPQWLQDAGGKPMSLGLDLSGGVHFLLQVDMEKFLGDRMR
                     SNQEAIRDLLVDARIRYVGRDWLDGRQLSIPFQTAEGRDEASGLIAETFDGFEIQERD
                     VDGRPGLRLVLDDAKIGELEDLAISQNLQSLRNRVNELGVSEPLVQRLGRSRIVLDLP
                     GIQDSARAKEIINKFANLEFRLVAAPDARSSQTENYDYEGAPVSLERRNIVTGDQVTN
                     AVQEYDPESGMPQVSITLDNDGGRAMNEVTKDHVGDSMAILFKELKVRTRRVVEDGEE
                     KLQPYTVEEKRLINVATIQSALGFRFRITGLGLGEARDLALLLRAGALAAPMYIVEER
                     TVGASLGEENIEKGRQSIVIGFLLVMVFMLVYYRWFGLAANVALTMNLVLIVAIMSVL
                     GATLTLPGIAGIVLTVGMAVDANVLIFSRIREELTDRPPQQAISAGFDRALLTILDAN
                     ITTFFVAIILFSVGSGPVKGFAVTLAVGVVTSMFTAIMGTRALVNLMYGGRSLERLSI
                     "
     gene            complement(2777..3118)
                     /gene="yajC"
                     /locus_tag="AC16QF_09275"
     CDS             complement(2777..3118)
                     /gene="yajC"
                     /locus_tag="AC16QF_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR6448789.1"
                     /translation="MNLFETTAYAADAGGPPPDAGMINLLFLGGFILIFYFLLWRPQS
                     KRRKEHQALMSGLAKGDEVVTTGGIVGQVTRVDDDFVKLQVANNIELRVQKSSVGATL
                     PKGTLKSLDGD"
     gene            complement(3105..4220)
                     /gene="tgt"
                     /locus_tag="AC16QF_09280"
     CDS             complement(3105..4220)
                     /gene="tgt"
                     /locus_tag="AC16QF_09280"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR6448790.1"
                     /translation="MSFELLGTDGAARRGRLKTAHGTVETPVFMPCGTYGTVKAMTPR
                     ALKEVGTQMLLGNTFHLMLRPGERLIESLGGIHEFMHWDGPVLTDSGGFQVWSLGELR
                     KISEEGVVFRSPVNGDRVELTPERSVAVQRALGSDVLMVFDECTPYPATEAEARLSME
                     LSMRWAERSLRAHQPSSGGLLFGIVQGGMFPALRQASLESLVELDFPGYAIGGLSVGE
                     SKSEMREVLDRLLPLMPTTKPRYLMGVGTPEDLVRAVGLGVDMFDCVMPTRNARNGYA
                     FTSAGTLKIRNARHRESDLPLDERCGCYTCANFSRAYLHHLDKCGEMLGSMLMTHHNL
                     HYYHWLMAELRASIESGKFRTLIETLRTDWNSPDEPI"
     gene            complement(4210..5232)
                     /gene="queA"
                     /locus_tag="AC16QF_09285"
     CDS             complement(4210..5232)
                     /gene="queA"
                     /locus_tag="AC16QF_09285"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR6448791.1"
                     /translation="MHPGDFDYELPNGQIAQAPASRRDASKLMHIAGDGIEHLGFHRF
                     PELLMPGDLLVLNDTRVIKARLHGVKDSGGQAEVLVERIEGETEALCQVRVSKALKPG
                     RFIQIGDAELEVLGRKGDFYSLRFPSDVLAFLEQRGETPLPPYIERAAGTVDADRYQT
                     VYGRRPGAVAAPTAGLHFTEDLLRQVRDRGVEIAEVTLHVGAGTFQPVRVDDISQHRM
                     HAERYEICSRAARALAECRERGGRIVAVGTTVVRTLESAAARSGEVEAGSGETDLFIT
                     PGFPFRVVDVLLTNFHLPRSTLLMLVCAFAGYERVMAAYAEAVDEGYRFFSYGDACFM
                     ERTDEL"
     gene            5362..5706
                     /locus_tag="AC16QF_09290"
     CDS             5362..5706
                     /locus_tag="AC16QF_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448792.1"
                     /translation="MIIRHMNRSYNRQEAEAEIRDRGLHEMVFEVPAVDNEPHWHDFD
                     AYLYLLDGTLHLRDVQAGVVHECSPGMRIDVPAKTVHAEQSKGYKVLLGCTRDPATFD
                     EGLNRDPATLPD"
     gene            complement(5747..6676)
                     /locus_tag="AC16QF_09295"
     CDS             complement(5747..6676)
                     /locus_tag="AC16QF_09295"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6448793.1"
                     /translation="MSDAEREAYARDGFVRLKNIYPLSWVELLEAQLEDVFEHQPHRA
                     MDQRSVTGGRTQGIRVDMAALANGMIAAVPDASIAVEGGRDAPIVGRSIVETDAASWH
                     SGIRRHHLEGPLPEIVADLTGTGKIVFYADQLFLKEPGSRIRTPFHQDEPYFLVQGEV
                     AVCWVPVDPVTRENGCMGYVRGSHRWGRLFKPSDFVTDDGTFPERDGIEHSGLETLPP
                     IDVEGPDVVYLEADPGDVIVHHWSTVHGAAGNVSGSATRRAASVRYALGDSRYFRRAS
                     SPEPFRDTLDLRDGDPLDRAPRFPVVWPRVRRR"
     gene            complement(6704..7528)
                     /locus_tag="AC16QF_09300"
     CDS             complement(6704..7528)
                     /locus_tag="AC16QF_09300"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HIR6448794.1"
                     /translation="MSGACLQRYCETSLNYGDGDSARTVQVTIRNARTTPDLEFEECG
                     FSRLDHVSEVHDWSDAAHLADVHMAEVRELAQGFLGCDHALAYPPIVRSPASARAVAD
                     YAPIEFVHSDFTDDYLDMIRDPDRAYGAFLAPALETIGVRQDEIAKAERVVMLQFWRN
                     VGARRPDRPLALCDAASVPRSDLFSILVPEYGGQRLEFETFGVRCPKDPDRHRWYTYP
                     DLTAEEVIVFRTYDSRCAAEGRPFWTPHSAFRDPRAGTDPAQRESVEMRVLCFFGL"
     gene            complement(7539..8225)
                     /locus_tag="AC16QF_09305"
     CDS             complement(7539..8225)
                     /locus_tag="AC16QF_09305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR6448795.1"
                     /translation="MQKLYTWFAVIGFGSVAASLMVGFRHDPAAPAVNYLLNAGLFLA
                     YMVVHYVMMTPGFKKLVSSNPAGSPAERRLYMVVAIASWIAVYLIHYPMPGPALALPG
                     WVTFLGGCAFMMSFLAFTEGTTFEGIRAFAGVPGHEQTHGASAEAPLQTEGSYASVRH
                     PMYRGACYMGLTSLLLHPNAAQLAWAGIMGLTFLTFIPIEEKQLLKGRGDEYREYMKQ
                     TPYRIFRGVW"
     gene            8359..9699
                     /locus_tag="AC16QF_09310"
     CDS             8359..9699
                     /locus_tag="AC16QF_09310"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6448796.1"
                     /translation="MTIEIVNHTPEPAVIANYSTARYVDRDYMEREWEHVWRRSWLLA
                     GLESDVQKPGDYFVFDMGREQILVTRTASGNVQGFYNVCQHRGNLLVAGERGHADSFR
                     CAYHAWTYDTEGSLKIIPYQERFARGVPCAERSLRKVHTDVWDGFVFVSLAEEPEPLL
                     DFLGPVVEMLAPYRFRNMVLVEDQTVHHKCNWKAVVDNFSELYHVDFLHPQHKRMVDC
                     CNDTVHLFPRGHTGLAVPGATVNPRFPIPDEPTDLQSAQLSSIGLDPADFRGRVMDVR
                     EAVQKRKREVGAEMGFDYSVFSEDQLSDVWQYNLFPNIIFSFTPEHCWVMRPRPHPTD
                     PQQCYFDKLSLLLFADPEINSKATSIVGPGRPEAISGTHLPDDYVRPERDVFDHQAII
                     SGEKTMTITIDQDIELLGGVQTGMASAGFDTVWLNDDEMRVQHFHNELDRLIGS"
     gene            complement(9743..10258)
                     /locus_tag="AC16QF_09315"
     CDS             complement(9743..10258)
                     /locus_tag="AC16QF_09315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6448797.1"
                     /translation="MSEKEYAEDYEDVTQYGLDEDKETELRQKQRECTFMWTNKQGEP
                     VGVIMSYLETDDGRLWLTGSEQRARFPAIRRDPRTCVCISSAGTDMGYGKTVTYKGIS
                     IIHDKSNRKIKDWFYPTFAKKIRAEQGDDRVQQFVEFLDSPRRVVVEFVPTKKIVYDG
                     AKMAAATPAIR"
     gene            complement(10344..11228)
                     /locus_tag="AC16QF_09320"
     CDS             complement(10344..11228)
                     /locus_tag="AC16QF_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR6448798.1"
                     /translation="MKVVATITLAAAFGLSSNASVAAALDERIDAAGAEVVEVVNRSG
                     SVTVTGGSDDVVVVSGSLGSNALGLDLDRDGDRIVVAVRYPEGDSGRNMASTLNISMP
                     RAAGLVVRSISADLEIGGIDGEQRLNTISGDIVTAVGSADVELRTVSGDMAITGRNEA
                     AVLKAASVSGDFGADAIAGQVDAATTSGDLQLLAGTLERARLASVSGDIEVRAQVASK
                     GSLVMESTSGDVAVVVEDAADISVEAETFSGSIDSCFDAETDGGFGPGESLRFRKGDG
                     DRSVRIMSFSGNVVVCDS"
     gene            complement(11225..11773)
                     /locus_tag="AC16QF_09325"
     CDS             complement(11225..11773)
                     /locus_tag="AC16QF_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448799.1"
                     /translation="MSDDRDDLEFLRRVRGLSGEVEPERDLWPEIRAALDEPGPRAKR
                     WRLPSALAASLLLGAVAASLLFRAGTAPEPAGVPAARPALLEVGLAPELMRSRAQLIA
                     SLDAELARMSPESQAAVAESLKEIEASLAKIHDELEHNPDSRLLLDLLVSTYLRQIAV
                     LGEMDGLARSVARSAQIERTET"
     gene            complement(11773..12333)
                     /locus_tag="AC16QF_09330"
     CDS             complement(11773..12333)
                     /locus_tag="AC16QF_09330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6448800.1"
                     /translation="MSMHEASSSQDTRADEALALLCQRAGAGDHGAFEELYRASVGRV
                     YGLCLRMTANVTAAEDATQATFVQAWRNLGRFRGEARVTTWLHRIAVNEVLGQQRREA
                     RHQHEPEEAWNEQASETAGASKDLDLERAIAGLPEQARNVFVLCGVYGYGHREAAGLL
                     GIAEGTSKAHYHTARRKLREALAGAE"
     gene            12407..12985
                     /locus_tag="AC16QF_09335"
     CDS             12407..12985
                     /locus_tag="AC16QF_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013346.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HIR6448801.1"
                     /translation="MTLDSFPPALTTFVLIGMAELGDKSQLVCMALAARHRAAPVLLG
                     ASLAFLILNALAVFLGAGLAVWLPDRLMAGLVALAFAGFGIHALTAGPGDDPEEHREH
                     RARGIFLTTLSLIFLAELGDKTQIAVAALGATLDPLQVWLGATLALFGLSALGVWIGR
                     SYLKRLPTIWLHRVSGLVFLGVGSLAAWRALT"
     gene            complement(13031..13222)
                     /locus_tag="AC16QF_09340"
     CDS             complement(13031..13222)
                     /locus_tag="AC16QF_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448802.1"
                     /translation="MVNNDLVQCLRSGARQRELDPGILDAAADALETLHRRNAELSEM
                     LVQLLPYAAADRLRHKTLE"
     gene            13512..14924
                     /locus_tag="AC16QF_09345"
     CDS             13512..14924
                     /locus_tag="AC16QF_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR6448803.1"
                     /translation="MPADREITAEYLIIGGGALGMAIADTLIGESDSSVIMVDRRHKP
                     GGHWHDAYPFVRLHGPSANYGVNSRQLGSDRIDEAGLNRGLFELATGSEIRTYFDEAM
                     RHGLLPTGRMHYLPMHEYNGDGQAVSLISGQATKVSARKKIVDATIAETQVPSRTSPN
                     FSVAEGATLIPPNGLAELGGSASTYVIIGAGKTAIDAVTWLMENDVDPGRLKWIRPRD
                     PWLLNRKMIQPSYDFFENTIGWLIASMEAAANADTVDDIFLHLESAGCMHRIDTSVIP
                     TMYRCAIVSEMELDQLRLVKDVVRMGHVRAIADNRVVLDDGELDLSPGTLFVNCSADG
                     IPRKPVQPIFQPGKIVPQYVRACSPTFSGALVAKVELLFGDDEEKNALTRPTPIPDSP
                     ADWVQIELNQALNSAAWRENKALSQWLAGSRLDQFTAMMLRAFSEGDEAKIGLLDRYR
                     TSIRPAVERMGKMVAASTSA"
     gene            15104..15781
                     /locus_tag="AC16QF_09350"
     CDS             15104..15781
                     /locus_tag="AC16QF_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaD family glycine zipper/OmpA-like
                     lipoprotein"
                     /protein_id="HIR6448804.1"
                     /translation="MKISCLGRMGAISICVAVGLNACTTNPYTGEREASNTAKGAGIG
                     AAIGAVVGVLSGDDADERRKNALIGAGVGALAGGAVGAYMDAQEAKLREQLANTGVSV
                     SRLGDDIVLNMPGNVTFDVNQDAVKSGFYPVLDSVGLVLEEYDKTLIDVNGHTDSTGT
                     VSYNMDLSGRRALSVADYLTNRGVARDRIFTQGYGPHYPLADNGTAEGRSLNRRVELV
                     LKPLTAG"
     gene            15886..16278
                     /locus_tag="AC16QF_09355"
     CDS             15886..16278
                     /locus_tag="AC16QF_09355"
                     /inference="COORDINATES: protein motif:HMM:NF019301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR6448805.1"
                     /translation="MANFMAGYTSQTYALLRIVAGFLFLWHGSQKLFSFPVDAMAGVP
                     AFITYGAGGIELIGGVLVMIGLFTRWAAFICSGTMAVAYWMAHGLNALFPIQNGGELA
                     ALYCFIFLYIAAKGAGSWSVDGARQQAG"
     gene            complement(16366..>16705)
                     /locus_tag="AC16QF_09360"
     CDS             complement(16366..>16705)
                     /locus_tag="AC16QF_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263500012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin domain-containing (seleno)protein"
                     /protein_id="HIR6448806.1"
                     /translation="VYAPAVKDWVAKGAESEHVQSAETVVGNIRAHSDDQLKADAAFR
                     LGNLFRLHGQQQKAEHYWKLAQSLNPDSINFIRQDLTLTEEGSAGETFMKFMGEYVSQ
                     GKDYYRPLDL"
BASE COUNT         3129 a         5460 c         5141 g         2975 t
ORIGIN      
        1 gacgagtagg tccccaccag aacgccgatg gtcagggcaa gggcaaaccc gcggatcatc
       61 tctccgccag cgaacagcag ggcgagcagc accagcagcg tggtcaacga cgtgaccaac
      121 gttcgtccca gggtctggtt gagcgacgta ttgatgatgt ccacggccgc gcctttacgg
      181 atcttccgga aattctcgcg aatgcggtcc gagacgacga tggtatcgtt cagcgagtag
      241 ccgatgacgg ccagaagggc cgcaaggacc ggaaggtcga acgtccactg cgccatggcg
      301 aatattccga gaacgatggc aacgtcgtga atgagcgcta ccacggcacc gacggaaaac
      361 tgcttggtga accggaacag gatgtagagc atcacgacaa acagcgcggc gagcaccgca
      421 agcccgccgt cctccgttag ctcctcaccc actgccggac ccacgtagtt cgactggcgc
      481 atgaccacgc cgggatagcg ggcactgaga accgcaagga tctcgtcgcc catgttggcc
      541 tgctcgccgt cttcctgggg cggcatgcgg atgagcagat cccgctcggt cccgaagtac
      601 tggacgacgc cgttgctgta gccgtcgttc tccagcagct cgcgcacggt gttcgggtta
      661 gtcggctcca cgaactccac ctcgaccaga gttccgccgg tgaagtcgag gccgaggttc
      721 aggccgaccg agaaaacggc gatcagagaa accagcacca ggaccatgga gataccaccg
      781 gcaatgcggc gcttgcccat gaaatcgatg ttcaggttca agggcttcat atggagagcc
      841 tctcgagcga gcgcccgccg tacatcaggt tgaccagcgc tcgcgtcccc atgatggccg
      901 tgaacatcga cgtcacgacc ccgaccgcca gcgtgacagc gaaccctttg acgggaccgc
      961 tgccgacgga aaagagaatg atggccacga agaaggtcgt gatgttcgcg tcgagaatcg
     1021 tcagcagagc cctgtcgaag ccggcagata tcgcctgctg agggggacgg tccgtgagct
     1081 cttcgcggat gcgggaaaag atcagcacgt tggcgtccac cgccatgccg accgtgagca
     1141 cgatccccgc gatgcctggg agcgtcagag tggcgccgag caccgacatg atggcaacga
     1201 tcagaaccag gttcatggtc agggcaacgt tagccgccag cccgaaccat cggtagtaga
     1261 ccagcatgaa caccatgacc agcaggaagc cgatgacgat ggactgacgc cccttctcga
     1321 tgttctcctc gccgaggctc gccccgacgg tccgctcctc gacgatgtac atgggagcgg
     1381 ccagggcgcc ggctcggagc agcagagcga gatcgcgggc ctcgcccaga cccagccccg
     1441 tgatgcggaa gcggaatccc agcgcgctct ggatggtggc cacgttgatc aggcgtttct
     1501 cctccacggt gtagggttgc agcttttcct cgccatcctc gaccacacga cgggtgcgga
     1561 ccttgagctc cttgaacaga atggccatgg agtccccgac atgatccttg gtcacctcgt
     1621 tcatcgcccg gccgccgtcg ttgtcgaggg tgatgctcac ctgaggcatg ccggactcgg
     1681 ggtcgtattc ctgaaccgcg ttggtcacct gatcaccagt gacgatgttg cgtcgctcca
     1741 gggacacggg cgccccttcg taatcgtaat tttcggtctg ggaggaacgg gcgtcgggcg
     1801 cggcgacgag acggaactcc agattggcga acttgttgat gatctccttc gcccgggcgc
     1861 tatcctggat accgggcagg tcgagcacga tacgggaccg cccgaggcgc tgaaccagag
     1921 gctcggaaac ccccagctcg ttgacccggt tgcgcaggct ctgcaggttc tgggatatgg
     1981 cgaggtcttc cagctccccg attttcgcat cgtcgagcac gagccgcagg cccggccggc
     2041 catccacgtc gcgctcctgg atctcaaagc cgtcgaatgt ctcggcgatc agcccgctgg
     2101 cctcgtccct gccctctgcg gtctggaaag gaatgctcag ctgcctgccg tcgagccagt
     2161 ctcgtcccac gtagcggatg cgggcatcta ccagcagatc gcggatggct tcctggttgc
     2221 tgcgcatccg gtcgccaagg aacttctcca tgtcgacctg aagcaggaaa tgcacgccgc
     2281 ccgagagatc gaggcctagc gacatgggct tgccgccggc atcctgaagc cactgcgggg
     2341 tggtcgaggc gcggttcagg gcaaccacgt agcggcccga cgactccggg ttgagctcgg
     2401 cggtggcgat gtcgcgcccg cgcagctgct gctcgtccgt gccgacccgg accagagcgg
     2461 aatcgccggc cagctcggtg ccctttaccg ttacgccgcg cgctgccagc gccgccgtca
     2521 cccgctccag cacctcctgg ttgagcgggt tggcttcgtt ggtactcttg atctgcagcg
     2581 cgggatcggg ctgaaacagg ttgggcgccg cgtatacgcc gccaatgacc acgacgaaca
     2641 ggatgaacag ataccgcccg acggagtgag tgttgggcgc tcggcgccgc gccttcgaca
     2701 acccgagcag gccttgtccc accacaccac tcgaattagc cacggctgga cgttctccgc
     2761 gcttgccagc cgcgcactaa tcgccgtcca gggatttcag cgtgccctta ggcagcgtcg
     2821 cgccgacgga gctcttctgc acgcgcagct cgatgttgtt tgccacctgg agcttgacga
     2881 aatcgtcatc caccctggtc acctgaccaa cgatgccgcc cgtggtgaca acctcatccc
     2941 ctttggccag gcccgacatc agcgcctgat gttccttgcg gcgcttgctc tgagggcgcc
     3001 acaggagaaa gtagaagatg aggatgaaac cgccgaggaa aagcaggttg atcatgccgg
     3061 catcgggcgg cggaccgccg gcatcggccg cgtaggcagt ggtctcaaat aggttcatcc
     3121 ggggagttcc agtcggttcg aagggtctcg atgagggtgc gaaatttacc gctttcgatg
     3181 gaggcgcgca attcagccat cagccagtgg tagtagtgga ggttgtggtg cgtcatcagc
     3241 atgctgccca gcatctcgcc gcatttgtcc agatgatgaa gataggcccg cgagaaattc
     3301 gcgcaggtgt agcagccgca gcgctcatcg aggggcaggt ccgattcccg atgcctggcg
     3361 ttgcggatct tcagggttcc cgccgacgtg aaggcgtagc cgttgcgcgc gttgcgcgtg
     3421 ggcatcacgc aatcgaacat gtccacgccg agccccactg cacgaacgag gtcctcaggc
     3481 gtgccgaccc ccatgagata tcttggtttg gtcgtgggca tcagcggcaa cagcctgtct
     3541 agaacctccc tcatctccga cttgctctcc cccaccgaga gcccgccgat ggcgtaaccg
     3601 gggaagtcga gctcgaccag tgactccaag ctcgcctgac gcagagccgg aaacatgccg
     3661 ccctggacga tcccgaagag gagaccaccg gacgagggtt ggtgcgcacg caggctgcgc
     3721 tccgcccagc gcatcgacag ctccatggac aggcgggcct cggcttccgt cgcggggtaa
     3781 ggcgtgcact cgtcgaacac catcaaaacg tcgctgccca gagcgcgctg gacggcaacc
     3841 gagcgctcgg gcgtaagctc gacacgatcg ccgttcaccg gcgagcggaa aaccacgccc
     3901 tcctcgctga tcttgcgcag ctctcccagg ctccaaacct gaaagccacc gctgtcggtg
     3961 aggactggac catcccagtg catgaactcg tgaatgccgc ccaaggattc aatgagccgt
     4021 tctccggggc gcagcatcag atgaaaggta ttgccgagca gcatctgcgt ccctacctcc
     4081 ttcagcgcgc gcggcgtcat ggccttgacg gtcccgtagg ttccgcaggg catgaaaacc
     4141 ggtgtctcga ccgtgccgtg cgccgttttc aggcgcccgc gccgggcagc gccgtcggtg
     4201 ccaagcaact caaagctcat cggttcgctc catgaagcaa gcatctccgt agctgaaaaa
     4261 gcgatatccc tcgtcgaccg cctccgcata ggcggccatc acccgttcgt aacctgcaaa
     4321 cgcgcagacg agcatcagca gggtggaccg cggcaggtgg aaattcgtga gcagcacatc
     4381 caccacgcga aacggaaatc cgggcgtgat gaacagatcg gtctcgcccg agcccgcctc
     4441 gacttcgccg cttcgagccg cggcactttc gagggtgcgg accaccgtgg tgcccacggc
     4501 gacgatccgg cctccgcgct cccggcactc ggccagagca cgagccgcgc ggctgcagat
     4561 ctcgtaccgc tcggcatgca tccggtgctg cgagatgtcg tccacccgaa cgggctggaa
     4621 cgtcccggcg cccacgtgga gagtgacttc ggcgatctcc acccctcggt cgcgaacctg
     4681 ccggagcaga tcttccgtga agtgcaggcc ggcggtgggc gccgccaccg cgccgggccg
     4741 gcgtccgtat acggtctggt agcgatcggc atccacggtc ccggccgctc gctcaatgta
     4801 gggcggcagc ggcgtttcgc cgcgttgttc caggaatgcg agcacgtctg acggaaagcg
     4861 caggctgtag aaatcccctt ttcgaccgag cacctcgagt tcggcatcgc cgatctggat
     4921 gaaccggcct ggtttgagcg ccttgctgac ccggacctga cacagcgcct cggtttcgcc
     4981 ctcgattcgc tcgaccagca cctcggcctg cccgccgctg tccttgacgc catgcagccg
     5041 cgccttgatg acacgggtgt cgttgagcac gagcagatcg ccgggcatca gcagctcggg
     5101 aaaccgatga aagccgaggt gctcgatccc atcgccggca atgtgcatga gcttgctggc
     5161 gtctctcctg gacgcgggag cctgggcaat ctgcccgttt gggagctcat agtcgaagtc
     5221 gcctgggtgc attggtttta ccgaccaacg gaactgcgca ggccgcggat tctaatccgg
     5281 cgcgtgcagg agcaaaagcg cgccctgctg gggcataatg gcagcgcttt cagccacgct
     5341 cacggaggcc cgggaaatca gatgatcatc aggcacatga accgctcgta caaccggcag
     5401 gaagccgagg cggaaattcg cgaccggggt ctccacgaga tggtcttcga ggtccccgcc
     5461 gtcgacaacg agccgcactg gcacgatttc gacgcctatc tctacctgct cgatggcacg
     5521 ctgcacctgc gggacgtgca agccggcgtg gtgcacgagt gctccccggg catgcgaatc
     5581 gacgtgccgg ccaagaccgt gcacgcagag caaagcaaag gctacaaggt gctgctcggc
     5641 tgtacccggg acccggcgac ctttgacgag gggctcaatc gggatcctgc taccctgccc
     5701 gactgatcgg ccggacgaag acggacccga ctagctgacg agccggctac cgccgccgca
     5761 cccgcggcca gaccaccgga aaccttggcg cgcgatccag agggtcgccg tcacgaaggt
     5821 cgagggtatc ccgaaacggc tccggggatg atgctctgcg gaagtagcgg gagtcgccta
     5881 gcgcgtagcg cacggaggcg gcccgccggg tggccgagcc ggagacgttc cccgcagcgc
     5941 catgaacggt cgaccagtga tgcacgatca cgtcgcccgg atccgcctcc agatagacca
     6001 cgtcagggcc ttcgacgtcg atgggcggca gggtctcgag accggagtgc tcaatgccgt
     6061 ccctctcggg gaatgtcccg tcgtccgtga cgaagtcgct gggcttgaac aggcgtcccc
     6121 agcggtgcga accgcgcacg tagcccatgc aaccgttctc ccgggtcacg ggatccacgg
     6181 gcacccagca cacggcaacc tcgccctgca cgagaaagta aggctcgtcc tggtgaaagg
     6241 gtgtacggat gcgcgagccg ggctccttga gaaacagctg gtccgcgtaa aagacaattt
     6301 tgcccgtgcc ggtgaggtcg gcaacgattt ccggcagcgg cccttcgaga tgatggcgcc
     6361 ggattccaga atgccagctg gcggcatccg tctccacgat gctgcgtccg acgatgggcg
     6421 cgtctcttcc tccctcgacg gcaatcgacg catccgggac cgccgctatc attccgttgg
     6481 caagcgccgc catgtccacc cgaatgcctt gcgtgcgtcc gcccgtcacc gaacgctggt
     6541 ccatggccct gtgaggttga tgttcgaaaa cgtcttcgag ctgggcttcg agcaactcga
     6601 cccaggacaa gggatagatg ttcttcagcc ggacgaaacc gtcccgtgca taggcctcgc
     6661 gctcggcgtc cgacacgtct ctgaccggcg agccgtccac ggcttagagg ccgaagaagc
     6721 agagcacgcg catctccacg ctttcgcgct gagcgggatc cgtacccgcg cgcggatctc
     6781 tgaatgcgga atgaggagtc cagaagggcc ggccctcggc cgcgcagcgg ctgtcgtaag
     6841 tgcgaaacac gatgacctct tcggcagtca ggtccgggta cgtgtaccag cgatgccggt
     6901 ccgggtcctt cggacagcgc acgccgaagg tttcgaactc cagccgctgg ccgccgtact
     6961 ccggcaccag gatggaaaac agatccgagc ggggcacgct ggccgcgtcg cacagggcga
     7021 gcggtcggtc cggtcgtcgg gcccccacgt tgcgccagaa ctgcagcatg accacgcgtt
     7081 ccgctttcgc gatctcatcc tgacggacac cgatggtctc cagagccggc gcgagaaagg
     7141 cgccgtaagc tcgatccggg tcccggatca tgtcgagata gtcgtcggtg aagtccgaat
     7201 gaacgaactc gatgggcgcg tagtcggcca ctgcgcgggc actggcaggg ctgcggacga
     7261 tcggcggata cgccagcgcg tgatcgcaac cgaggaaccc ctgcgccagt tctcgcactt
     7321 cggccatgtg aacgtcggca aggtgggcag cgtcgctcca gtcgtggacc tccgagacat
     7381 gatccagtcg ggaaaaaccg cactcctcga attcgagatc cggcgtcgtt cttgcattgc
     7441 ggatcgtaac ctggaccgtt cgggccgaat cgccgtcacc gtagttcagc gatgtttcgc
     7501 agtagcgctg cagacaagcg ccggacatcg cgcggcgcct accagacgcc tcggaatatg
     7561 cggtaaggcg tctgcttcat gtactccctg tactcatcgc cacgtccttt gagcaactgc
     7621 ttctcctcga tgggtatgaa cgtcaggaac gtgagcccca taatccccgc ccaggccagc
     7681 tgcgccgcgt tgggatgaag cagcagggag gtcagcccca tgtaacacgc gccccggtac
     7741 attggatgtc tgacgctggc gtaggaaccc tccgtctgca gcggcgcttc ggcagaagcg
     7801 ccatgcgtct gctcgtgtcc cggtactccc gcaaaggccc gaatcccttc gaacgtggtc
     7861 ccttctgtga acgcgagaaa agacatcatg aacgcgcaac ctcctagaaa cgtcacccag
     7921 cccggaagcg ccagcgcggg gcccggcatc ggatagtgaa tcagatacac ggcgatccag
     7981 gaggcgatgg ccaccaccat gtagagcctg cgctccgcgg ggcttcccgc cggattgctc
     8041 gaaaccagct tcttgaaccc gggcgtcatc atcacgtagt ggacgaccat gtaggccaag
     8101 aacaagccgg cgttgagaag gtagttgacg gccggagctg cgggatcatg gcgaaacccc
     8161 accatgagag aggcggccac cgaaccgaag ccaatgaccg caaaccaggt atacagcttc
     8221 tgcatgctct catcccctca gaaaagagtt tcccgaatat agccgaaggc gccgcgcagc
     8281 gcctccccgc aagggaggcg tttccagccg cgaccgcggc tggcatcatt gggcgcagga
     8341 gaagccagcg gatccgtcat gaccatcgag atcgtcaacc acacccccga acctgccgtc
     8401 atcgccaact actcgacggc ccgctacgtg gaccgcgact acatggagcg ggagtgggag
     8461 cacgtctggc gcaggtcctg gctgcttgcc gggttggaga gcgacgtgca gaagcccggc
     8521 gactatttcg tcttcgacat ggggcgcgag cagattctgg tgacccgaac ggcttccggc
     8581 aacgtgcagg gcttctacaa cgtctgtcag catcgaggca acctgctggt ggccggcgag
     8641 cgcggtcacg ccgacagctt ccgctgcgcc taccacgcgt ggacgtacga taccgaaggc
     8701 tctctgaaga tcattcccta ccaggagcgg ttcgccagag gtgtcccgtg cgccgagcga
     8761 tcgctgcgga aggtgcacac ggacgtatgg gacggtttcg tgttcgtctc cctcgccgag
     8821 gagcctgagc cgctgctcga ttttctgggt ccggtggtgg agatgctcgc gccttaccgc
     8881 tttcgcaaca tggttctggt tgaggaccag acggtacacc acaagtgcaa ctggaaggcc
     8941 gtcgtcgaca atttcagcga gctttaccac gtggactttc tgcatcccca gcacaaacga
     9001 atggtggact gctgcaacga cacggtgcat ctgtttcccc gcgggcacac gggcctcgcc
     9061 gtgccgggcg cgacggtgaa cccgcgattc ccgatccccg acgagccgac ggaccttcag
     9121 tccgcgcagc tatcgagcat cggccttgat ccggccgatt tccgaggaag ggtgatggac
     9181 gtgcgggagg cggtgcagaa gcgtaagcgc gaggtgggcg cggagatggg tttcgactac
     9241 tccgttttct cggaagatca gctctccgat gtctggcagt acaacctgtt cccaaacatc
     9301 attttctcct tcacgccgga gcactgctgg gtaatgcgcc cgcggcccca cccgaccgat
     9361 ccgcagcagt gctatttcga caagctctca ctgctgcttt tcgccgatcc cgagatcaac
     9421 tccaaagcca cgtcgatcgt gggtcccggg aggccggagg caatatccgg cacgcacctg
     9481 cctgacgact acgtccgccc ggagcgcgac gtgttcgatc atcaggccat catcagcggc
     9541 gagaagacca tgaccatcac catcgatcag gacatcgaac tgctaggcgg cgttcagacc
     9601 ggtatggcgt ctgcgggttt cgatacggtc tggctgaacg acgacgagat gcgcgtgcag
     9661 cactttcaca acgagctgga ccgactcatc ggcagctgac atcgcccggc gtgggccttc
     9721 cgggggcatc tccgatggcc gcttaccgaa tcgccggggt tgccgccgcc atcttggcac
     9781 cgtcgtagac gatcttcttc gttgggacga actccacgac gacccgccgg ggactatcga
     9841 ggaactccac gaactgctgc acgcggtcat cgccctgttc tgcccgaatc ttctttgcaa
     9901 aggtcgggta gaaccagtcc ttgatcttgc ggttgctctt gtcgtggatg atcgagattc
     9961 ccttgtaggt caccgtcttc ccgtaaccca tgtccgtccc ggcgctggag atgcatacgc
    10021 acgtcctggg atcgcgccgg atggcgggaa accgtgcccg ctgctccgac cccgtgagcc
    10081 agagacggcc atcgtcggtc tcgagatagc tcatgatcac gcctacaggc tcgccctgct
    10141 tgttggtcca catgaacgtg cactcgcgct gcttctgccg aagctccgtc tccttgtcct
    10201 cgtccagccc gtactgcgtg acgtcctcgt aatcctccgc gtattccttt tccgacatgg
    10261 ctgcgcttcc ccggtgataa agcttcgagt ctagcccgtc acggtcccgg cgcaaacaag
    10321 ctcgagccca cctgcggatc ggctcaggag tcgcagacca ccacgttgcc gctgaaactc
    10381 atgattcgta cgctgcgatc gccgtcgccc tttctgaaac gcagcgactc gcccgggccg
    10441 aagcccccgt cggtctcggc atcgaagcag ctgtcgatgg agccgctgaa ggtctcggcc
    10501 tctacggaga tgtcagctgc atcctcaacc acgacggcaa cgtcgccgct ggtcgactcc
    10561 atgacgaggc tgcctttcga cgcaacctgg gcgcgaacct cgatgtcgcc gctgaccgag
    10621 gcaagacgtg cccgctccag cgttccggcc agcagctgga gatcgccgct cgtcgtggcc
    10681 gcatccacct gcccggcaat ggcgtcggcg ccgaaatcac cgctcacgct cgctgccttg
    10741 agcacggcgg cctcgttccg gccagtgatc gccatgtcgc ccgagaccgt cctcaattcc
    10801 acatccgcgc tcccgacagc ggtgacgatg tctcccgaga tcgtgttcag gcgttgctct
    10861 ccgtcaatcc cgccgatctc cagatcggcg ctgatcgatc gcacgacgag accggccgcc
    10921 ctcggcatcg agatgttcaa cgtgctcgcc atgttcctgc ccgagtcacc ctccgggtat
    10981 cgaacggcga cgacgatccg atcgccgtcc cgatccagat ccaggcccag ggcgttgctg
    11041 ccaagggagc ccgagaccac caccacgtca tcggagccac ccgtaacggt gaccgatcca
    11101 gaccggttga cgacctcgac gacctcggcg ccggctgcgt cgatacgttc gtccagagcg
    11161 gccgccactg acgcgtttga gctcagaccg aacgcggcgg ccaatgtgat cgttgcgacg
    11221 actttcatgt ttctgtcctc tcaatctggg ccgagcgggc cacggagcgg gcaagcccgt
    11281 ccatttcgcc cagcacggct atctgccgaa gataggtgga gacgagcagg tcgagcagca
    11341 gtcggctatc ggggttgtgc tcgagctcgt cgtgaatttt cgcaagagac gcttcgatct
    11401 ccttgaggct ctccgcgacc gcggcctggg actccggcga catccgcgcc agctccgcat
    11461 ccagggacgc gatcagctgc gcccgggacc gcatgagctc tggcgcgagc ccgacctcca
    11521 gaagtgccgg acgggcagcc ggcacgccgg ccggctcggg agccgtcccg gcgcggaata
    11581 gcagcgacgc ggcaacagcg cctagaagca gcgacgcggc aagcgccgaa ggcagacgcc
    11641 agcgcttcgc gcggggcccc ggctcatcca gcgctgcgcg gatttccggc cagagatccc
    11701 gctcgggctc gacctccccg gaaagcccgc gaacgcgccg caggaattcc aggtcgtcgc
    11761 gatcgtcact catcattcag ctcccgccag cgcttctctc aacttgcgcc tggccgtgtg
    11821 gtaatgcgcc ttgctcgtgc cttccgcgat acccagcagg ccagccgctt cacgatggcc
    11881 gtacccgtag acgccgcata gcacgaatac gttgcgggcc tgctccggca agccggcgat
    11941 tgcccgttcc agatccagat ctttcgacgc tcccgcagtt tccgacgcct gctcgttcca
    12001 ggcttcctcc ggttcgtgct gatgccgtgc ttcccgacgc tgttgaccca gcacctcgtt
    12061 cacggcgatc cgatgcagcc aagtggtcac gcgggcctca ccacggaagc ggccaagatt
    12121 gcgccaggcc tggacaaagg tcgcctgggt agcgtcttcc gccgcggtta cgttcgccgt
    12181 catgcgcagg cacaacccgt agacccggcc aaccgatgcg cggtaaagct cctcgaaagc
    12241 cccatgatcg ccggcccccg cccgctggca aagcagcgca agcgcttcat ccgcgcgggt
    12301 gtcctgtgat gaactagcct catgcatact catcattatc atccctgcaa gataagatgc
    12361 agcctgcgcg agaagggttt agtcgcgttt gcggatccag gatctgatga ctctcgattc
    12421 tttcccccca gccctgacca ccttcgtgct catcggcatg gcggagctgg gcgacaagag
    12481 ccagctcgtc tgcatggcgc tggcggcacg ccatcgagcc gctcccgtgc tgctgggcgc
    12541 ttccctggct tttctgatcc tgaacgccct cgccgtcttc ctcggcgccg gcctggccgt
    12601 ctggctgccc gatcgcttga tggcgggcct ggtggctctg gccttcgccg ggttcgggat
    12661 tcacgccctg acggctgggc ccggtgacga ccccgaggaa caccgggagc accgtgcccg
    12721 aggaattttc ctgactacgc tgagcttgat attcctggca gagctgggag acaaaaccca
    12781 gattgccgtc gccgcgctcg gcgccacgct cgacccgcta caggtgtggc tgggagctac
    12841 cctcgcgctc ttcgggctgt cggcgctcgg tgtatggatc gggcgcagct atctcaagcg
    12901 cctgccgacg atctggcttc accgtgtgag cgggttggtc ttcctggggg ttggttcgct
    12961 cgcggcgtgg cgggcgctga cctgaacgga tcaaagctcg agacccacgc ccgctgctgc
    13021 cagcagcgca tcactcgagc gtcttgtggc gcaggcggtc ggcggccgcg tacggcagga
    13081 gctgaacgag catctcggac agttccgcat tacgcctgtg cagcgtctcc agagcgtccg
    13141 cggccgcgtc cagaatgcca gggtcgagtt ctctttgacg cgcgccgctg cgcaagcact
    13201 gaacgagatc gttgttaacc acggtcgagt ctccaaataa aacggcacgc atcagtccac
    13261 gcacgccaga tgaaacgtat aggcgcgtcc ggttcgccat ggtcggggtt aacagaaagt
    13321 tacccactta ccacacgctc agtccgtgac gcagcgcaca tagtggcgcc gacgtaatcc
    13381 gcagcgaagg tgagaacccg gcgcgatcaa ccttcgcgac ccaaccccgg cttgcgttcc
    13441 tttcgtccac ttgcgggaga ccgacgatcc atgcaacctg ccgcgggata gtctgggcaa
    13501 ggagcgagga gatgcctgca gacagagaaa ttacagccga gtatctcatc atcggcggag
    13561 gcgccctcgg catggccatc gccgatacgt tgatcggcga aagcgacagc agcgtcatca
    13621 tggtcgatcg acgacataag cccggcgggc actggcacga cgcctacccc ttcgtgcggt
    13681 tgcacggccc ctctgccaac tacggcgtga actcgcggca actgggcagc gaccggatcg
    13741 acgaagccgg cctgaatcgg ggcctcttcg agctggccac gggctcagag atccgaacct
    13801 atttcgacga agcgatgcgc cacgggttgc tcccgaccgg ccggatgcac tatctgccca
    13861 tgcacgaata caacggcgac ggccaggccg tatccctgat cagcggccaa gctaccaagg
    13921 tgagcgcgcg aaagaaaatc gtcgacgcga ccattgccga gacgcaggtg ccttcccgaa
    13981 cgtccccgaa tttttccgtc gctgaaggcg ccacgctgat acctccgaac ggcctggcgg
    14041 aactcggcgg ctcggcgagc acctacgtga tcattggagc gggaaagacg gctatcgacg
    14101 ccgtgacctg gctgatggaa aacgacgtcg atcccggccg gctgaagtgg atccgcccca
    14161 gggacccctg gttgctcaac agaaagatga ttcagccaag ctacgatttc ttcgagaaca
    14221 cgattggctg gctgatagcc agcatggaag cagccgcaaa cgccgacacg gtcgacgaca
    14281 tcttcctcca tctggaaagt gccgggtgca tgcatcgcat cgacacatcc gtaattccaa
    14341 cgatgtaccg ttgcgcgatc gtcagcgaga tggagctcga tcaattgagg ctcgtgaagg
    14401 acgtcgtgag gatgggccat gtgcgagcga ttgcagataa ccgcgtcgtt ctcgacgacg
    14461 gcgaactcga cctgtcgccg gggaccctct tcgtcaactg cagcgcggac ggcatccctc
    14521 gcaagcccgt gcaaccgatt ttccaacccg ggaaaatcgt gcctcaatac gtgcgcgcct
    14581 gctcgcccac gttcagcggc gcgctcgtcg caaaggtcga gctccttttc ggcgacgacg
    14641 aagaaaagaa cgccctcacc aggcccacac ccattccgga ttccccggca gactgggttc
    14701 agatcgagct caatcaagcg ctgaattccg ccgcctggcg cgagaacaag gcgttgtcgc
    14761 agtggctggc aggctcccgc cttgatcagt tcacggcgat gatgttgcgc gcattctccg
    14821 agggcgacga ggcgaagata gggttgctgg acaggtaccg cacgtcgatc cggcccgcgg
    14881 tggagcgaat gggcaagatg gttgcggcca gcacgtcggc ctgacggaac ccgcctctat
    14941 tgccccgata tttattgccc cgatattgcc ccgatccgtc gtcacctctc gtgtcttgaa
    15001 agccacaaca ctcggtttcc cgactcccgt tcacgctttc ttcaggattg caggtttaca
    15061 cttccccgcg ttaaaggcgc ctacagccgc agaggagcga ttcatgaaga taagttgcct
    15121 gggaagaatg ggggccattt caatctgtgt cgctgtcggg ttgaacgctt gcaccaccaa
    15181 tccgtatacc ggggaacgtg aagcgtccaa tactgccaag ggcgcaggca tcggcgcggc
    15241 catcggggcc gtggttggcg tgctgagcgg cgacgacgcg gacgagcgtc gcaagaatgc
    15301 gcttatcggc gccggtgtcg gcgcgctcgc gggcggcgcc gtgggagctt acatggacgc
    15361 ccaggaagcc aagctccggg agcaactcgc caacaccggc gtcagcgtga gtcggctcgg
    15421 cgacgacatc gtgctgaaca tgcccggcaa cgtgacgttc gacgtgaatc aggacgccgt
    15481 aaagtccggc ttctatccgg tgctcgactc ggtcggcctg gtgctggagg aatacgacaa
    15541 gaccctgatc gacgtaaacg ggcatacgga cagcacgggc accgtcagct acaacatgga
    15601 cctttccgga cgacgcgccc tgagcgttgc cgattatctg accaaccggg gcgtggcacg
    15661 cgatcgcatc ttcactcagg gttacggccc acactatccc cttgccgaca acggcaccgc
    15721 agagggacgt tcactcaacc ggcgggtcga gctggttctg aaaccgctga cggccgggta
    15781 atcggcccgc cccccgcgtc cggcagcgcg ccgccgcttc gccctgttcg gcaactgggt
    15841 tctgtctggt agtattgccc agagagacga ggggaggatc gttcgatggc aaatttcatg
    15901 gctgggtata ccagtcaaac ctatgctctg ctgcgtatcg tggccggttt tctgttcctg
    15961 tggcacggca gccagaagct tttttcgttc ccggtggatg ccatggccgg cgtgccggct
    16021 ttcatcacct acggagccgg cgggatagag ctcatcggcg gcgtgctggt gatgatcggg
    16081 ctgttcacgc gctgggccgc cttcatctgc agcgggacca tggcagtcgc ctattggatg
    16141 gctcacggct tgaacgcgct gtttcccatc cagaacggtg gcgagctggc cgcactgtac
    16201 tgcttcatct tcctttacat cgcggccaaa ggcgccggga gctggagcgt ggacggcgcc
    16261 cgtcaacagg caggctgacc tcgtctggca ggagggccgg taacctgaaa aaaccggcca
    16321 ggccgtcacg aacggcgaga ctgagccggg ctgcctggat tccgcttaga ggtccagcgg
    16381 ccggtagtag tccttgccct ggctgacgta ctcgcccatg aatttcatga aggtctcgcc
    16441 ggccgaaccc tcttccgtca gagtcaggtc ctgacggatg aagttgatgc tgtcgggatt
    16501 cagcgactgg gccagcttcc agtagtgctc cgccttctgc tgctggccgt ggagcctgaa
    16561 caggttgccg aggcggaacg ccgcgtccgc cttcagctga tcgtcgctgt gtgctcgaat
    16621 gttaccgacg accgtctcag cggactgaac gtgctcactt tcagcacctt tagccaccca
    16681 gtctttgacc gccggggcat agacg
//
LOCUS       DBNHLM010000206        10868 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1586139, whole genome shotgun sequence.
ACCESSION   DBNHLM010000206 DBNHLM010000000
VERSION     DBNHLM010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10868)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10868)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10868
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1586139"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="AC16QF_09365"
     CDS             <1..438
                     /locus_tag="AC16QF_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR6448807.1"
                     /translation="NGTDRELEVPDNTLLVDLLREHLRLTGTHVGCDTSQCGACTVHL
                     NGRSVKSCTVLAAHAEGAEVVTIEGIGAPDDLHPMQEAFRDCHALQCGFCTPGMIMSA
                     IDLVNQSDDLDNQAVREGLEGNICRCTGYHNIVRAVLQAAEKM"
     gene            448..2796
                     /locus_tag="AC16QF_09370"
     CDS             448..2796
                     /locus_tag="AC16QF_09370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6448808.1"
                     /translation="MSEAAATGIGASVRRREDVRFITGSGRFTDDLTQPGQTYAVFLR
                     SPYARARIGEIDVSEALATEGVIAVLTGRDMAADGLGDLPCGWLVKQKDGSDMISAPH
                     PPLATEVANYVGEPYGLVIAESLAAARQAAECVMADFEELDAVVDLATAVDSSQIHEG
                     VPGNRAYDWEIGDREATEAALAGAAHVTRIDLTNNRLIPNAMEPRAALGVYDPAGDDY
                     TLYTTSQNPHLERLVLSAFVQVAPEHKLRVIAPDVGGGFGSKIFIYAEETAVIWASRR
                     VGRPVKWTAERSESFLADAHGRDHITHAELGLDAEGNFTALKVQTSANLGAYLSTFGS
                     SVPTYLYGTLMAGQYRTPNIYVEVQGIYTNTAPVDAYRGAGRPEATYVVERIVETAAR
                     ELGTDPAELRRRNMIRPDQFPYETPVALVYDTGDYEAGLDRALALVDYAGFPARRAAS
                     EAAGKRRGIGLACYIEACGLAPSQLAIQLGAGVGLYESGEVRVNPTGSVTVFTGSHSH
                     GQGHETTFAQVVSDRLGVPFESVEVVHGDTGRVEFGLGTYGSRSLAVGGSALIAATDK
                     VIAKGKRIAAHMLEAQESDVEFADGNFVVRDSNQSLSFGEVAFAAYVPANYPEDLEPG
                     LSEKAFYDPENFTYPAGTHIAEVEIEEETGVVRLVNFVAVDDFGHVINPMIVDGQVHG
                     GLAQGAGQALLEHGVYDDYGQLQTGSYMDYCMPRADDVPDFVVDTTVTPCTHNPLGVK
                     GCGEAGAIGSPAAIMNAVTDALGVNDISMPATAEKVWRALRA"
     gene            2805..3602
                     /locus_tag="AC16QF_09375"
     CDS             2805..3602
                     /locus_tag="AC16QF_09375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR6448809.1"
                     /translation="MYQFNYHRPASVEEAVGLFESMDDGIYMAGGQTLIPTMKQRLAA
                     PTDVIDLSGIEALSGIEVSADRVSIGAFTVHDAVASSPEIRATLPVLAFLAGQIGDGQ
                     VRNRGTLGGSVANSDPAADYPAALIGLGATVHTQTRSIEADDYFKDLFETALNPGELI
                     TRIDFPVPTRAAYRKFPNPASRYAVVGVLVADFGGKVRVGVTGAGPCAFRATAIEELL
                     SRNLDPSALDGVEVPDAGFNNDLHASAEYRAHLVKVMAKRAVADLLS"
     gene            complement(3652..4425)
                     /locus_tag="AC16QF_09380"
     CDS             complement(3652..4425)
                     /locus_tag="AC16QF_09380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6448810.1"
                     /translation="MDVSGKIVVVTGGASGIGRAMAQRFHAEGAGKVIVADLDGDGAR
                     RVADEIDGSSMQVDVGREADLVALIEHAESEFGPIDLFCSNAGIGIGKGLDEPDEVWD
                     TIWKVNTMSHVYAARHMVPRMKARGGGYLLNTASAAGLLTQIGSVTYAVTKHAAVALA
                     EWIAITHARDGIKVSVLCPQAVRTPMTADGPGVAGVDGMIEPEEAAECVIEALREERF
                     LVLPHPEVATYIQRKTSDYDRWLNGMRRLQDLYVAEEGE"
     gene            4565..5548
                     /locus_tag="AC16QF_09385"
     CDS             4565..5548
                     /locus_tag="AC16QF_09385"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6448811.1"
                     /translation="MSSKLTTRPPALTWLILGSLLAVLSLPAISSEPARPLSAHHVIV
                     DGVPLAVWRKAPASAKRVVLLVHGRTWSARPNFDLNVPGEDLSLMDGLVEEGIATYAL
                     DMRGYGDTPRDASGWLTPSRAAADVIAVLEWIAQRNPGLPGPYLFGYSRGAMVAQLAA
                     QQQPGRISGLIVFGYPLRQDSAVNPPGLTTEPARIANSAADARSDFIAPGAARRETID
                     AFVESALKHDPVRVDWRAYPEWLALDAARVTVPTLLVDAEHDPLTDPKVLSRFFAGLA
                     TADKAWVSIPGADHAAFLEEPRDYFRRAVAAFMLGERAAVEVISADLLDFD"
     gene            5670..7451
                     /gene="pabB"
                     /locus_tag="AC16QF_09390"
     CDS             5670..7451
                     /gene="pabB"
                     /locus_tag="AC16QF_09390"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - aminodeoxychorismate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008153 - 4-aminobenzoate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HIR6448812.1"
                     /translation="MSTDVIVYDAVAQQWLRFCDPVELLIADREDQVLDVVAEIESRT
                     VGDGLFAAGFLTYEAAPGIARELVTHRPHRLLPLAWFGLFRRVLPSADLPLAPGDCLG
                     GWSASVTRKDYDSAIARIRDYIAAGDTYQVNYSFRLRARLNDSPAALFANMVSSQGPG
                     LAAFIDTPGFAVASASHELFFARDGDELVSRPMKGTAPRGLRDQTDRDQAGWLAASAK
                     NRAENLMITDMVRNDLGRIARIGSVVADDLFALERYPTVWQMTSTVRARSTASLPDVM
                     GALFPAASITGAPKRRTMEIIRELEHEPRGIYTGTIGFVRPEGRCQFNVAIRTAAIDK
                     TTSEAEYGVGGGIVWDSRAEDEYRECFAKARIVTGGRREFELLETLAWHPRDGFRRLE
                     EHLERLHSAADYFCWSLSTGKAQALLEAAASAYREPMRVRLLADRSGHVRLEASPLKP
                     LPEPYRVALSEPMEDLQSEFVYHKTTQRSVYDKALDAARSVLPDLDDVLLVNGDGQVT
                     ESTIANVAVEVDGRLLTPELGCGLLPGIERQVLVADKILEEARVPVSLLVEAERIWLL
                     NSVRGMWPATLVATEQGGSDLRSEFPG"
     gene            complement(7455..7530)
                     /locus_tag="AC16QF_09395"
     tRNA            complement(7455..7530)
                     /locus_tag="AC16QF_09395"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7495..7497),aa:Gly,seq:ccc)
     gene            complement(7606..8034)
                     /locus_tag="AC16QF_09400"
     CDS             complement(7606..8034)
                     /locus_tag="AC16QF_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR6448813.1"
                     /translation="MPIKNVFEAIQREKANIENLSVEELKQALADDPDLLLLDIREIQ
                     ERVDLGTIPGSVHVPRGMLEFWADPASPYFRDYFQEDRRTVVFCAGGGRSVLAVRALQ
                     DMGFTDVAHLEPGFGGWKKAAEPIEDVASSSRWVRRADDD"
     gene            8165..9526
                     /locus_tag="AC16QF_09405"
     CDS             8165..9526
                     /locus_tag="AC16QF_09405"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HIR6448814.1"
                     /translation="MRHTLKCAVFGWLLLGSLTAAAALPLGLPGGEQLPSLAPMLDQA
                     TPAVVNIATYTTVQVRNPLLEDPFFRRFFNVPDQRRYRRTQSAGSGVVIDAGKGFIVT
                     NNHVVERADEISVTLADGRSMTAELVGSDPQVDLAVLKVEADDLQQIGFADSGELRVG
                     DFVVAIGNPFGLRQTVTSGIISALGRSGLGIEGYEDFIQTDASINPGNSGGALVDLAG
                     RLVGINTAILAPSGGNVGIGFAIPSNMVRAIMDQLIEHGEVRRGYLGLAVQGLNVDLA
                     EAFGVDRREGVVVVEVEPDSAAESGGLQAGDIITRVGDREIGKIADFHSQAAVTFVGD
                     QVRIEVLRDGQPQQVTLKIGEDELEKVRGRRIDRRLAGTQLQNFRNQDEPGMGAGVLV
                     LDIDPDSDAYRFGLRPGDILVAANRRAIVNLAELREVARLDDGQLLLRVYRSGQFGYV
                     AIR"
     gene            complement(9547..10629)
                     /locus_tag="AC16QF_09410"
     CDS             complement(9547..10629)
                     /locus_tag="AC16QF_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HIR6448815.1"
                     /translation="MKAAVLREINKPLEIEDIQHGDPGPREVLVRTVAAGVCHSDLHF
                     QNGSYPYPLPAVLGHESAGIVEAVGSDVNYVKPGDHVITCLSAFCGHCEYCLTGHMSL
                     CQEPELQRPMDQPQRLNKGDENIWQFLNLSSFAEYMLVHEHAIAKIREDMPLDRAALI
                     GCGVTTGVGAVIHTAKVEPGSTVAVIGCGGIGLSAINGAAIAGAARIIAIDTVESKLD
                     LARKFGATDVVNARESDVIEAVREMTGGGVHYSFEAIGLKQTAEQSFKILQRGGTATV
                     IGMIPVGTNIELHGPEFLMERKMQGSNMGSNRFRVDMPRFVEFYLQGRLHLDDMISRR
                     IRLEDVNDGLAALETGELARSVIMFD"
BASE COUNT         1805 a         3516 c         3645 g         1902 t
ORIGIN      
        1 aatggtaccg accgcgaact cgaggtgccg gacaacacgc tcctggtgga cctcctgcgg
       61 gagcacctca ggctcacggg cacccacgtc ggctgcgata cgagccagtg tggcgcgtgc
      121 accgtgcatc tcaatggccg ctcggtcaag tcgtgcacgg tgctcgccgc acacgccgaa
      181 ggcgccgagg tggtgaccat cgagggaatc ggcgcgccag acgacctgca ccccatgcag
      241 gaggcgttcc gcgactgcca cgcgcttcag tgcgggttct gcacgccggg gatgatcatg
      301 agcgccatcg atctggtgaa tcagtcggac gacctggata accaggcggt ccgggaaggc
      361 ctggaaggca acatctgccg gtgcacgggt taccacaaca tcgtgcgtgc cgtgctgcaa
      421 gcagccgaga agatgtgagg attcgagatg agtgaagcag cggcaacggg gatcggagcg
      481 tcggtcaggc gtcgcgagga cgtccgtttc ataaccggca gcgggcgctt tacggacgac
      541 ctgacgcagc cgggccagac ctatgccgta ttcctgcgca gcccctacgc gcgcgccagg
      601 atcggcgaga tcgacgtgag cgaagcgctt gccactgagg gcgtgatcgc cgtgctcacc
      661 ggccgggata tggcagccga cggcctgggt gaccttccct gcgggtggct cgtcaagcag
      721 aaggacggct cggacatgat ctccgctccg cacccgccgc tggccaccga agtcgccaac
      781 tacgtgggcg aaccctacgg attggtgatc gcggagtcac tggccgccgc caggcaggct
      841 gctgagtgcg tcatggcgga tttcgaggag ctggacgcgg tagtcgacct ggcgaccgcc
      901 gtcgattcca gccagattca cgagggcgtg cccgggaacc gggcctacga ttgggagatc
      961 ggcgaccggg aggccacgga agcggcgctg gccggagccg cccacgtcac ccggatcgat
     1021 ctgaccaaca accgcctgat acctaacgcc atggagcctc gcgccgctct gggcgtatac
     1081 gatccggccg gcgacgatta cacgctctac accaccagcc agaaccccca cctcgagcgg
     1141 ctggtgttgt ccgccttcgt tcaggtcgca ccggagcaca agctgagggt catcgccccg
     1201 gacgtgggcg gtggtttcgg ttccaagata ttcatctacg cggaggagac cgccgtcatc
     1261 tgggcatcgc gcagggtcgg tcggccggtg aagtggacgg cggagcgcag cgagtcgttt
     1321 ctcgccgacg cccacggtcg ggatcacatc acccatgcgg agcttggcct ggacgcagaa
     1381 ggtaacttca cggcgctgaa agtgcagacc agcgcgaatc tcggcgccta tctgtccacg
     1441 ttcggctcct ccgtcccgac ctatctctac gggacgctga tggccggcca gtaccggacc
     1501 ccgaacatct acgtcgaggt gcagggcatc tacaccaaca ccgcaccggt agacgcctac
     1561 cgcggagccg ggcgtccgga ggccacctat gtggtcgagc ggatcgttga gactgccgcc
     1621 agggagctcg gcacggatcc ggcggagctg cggcggcgca acatgatccg gccggatcag
     1681 ttcccctacg agacgccggt ggcgctggtc tacgacaccg gcgactacga agccggtctg
     1741 gacagagcgc ttgcgctcgt cgactacgcc ggcttcccgg ctcgccgcgc tgcttccgaa
     1801 gcggcaggca agcggcgtgg cattggcctt gcgtgctaca tcgaagcctg cggcctcgcg
     1861 ccctcgcagc tggccatcca gctcggcgca ggcgtcgggc tctacgagag cggcgaggtg
     1921 agggtgaacc cgacgggttc cgtcaccgtc ttcaccggat ctcacagcca cggccagggt
     1981 cacgagacca cctttgccca ggtggtgtct gaccggctcg gcgtgccgtt tgagagcgtc
     2041 gaggtggtgc atggcgacac cggccgcgtc gagttcggcc tcggtaccta cgggtcgagg
     2101 tcgttggcgg ttggtggttc ggcgttgatc gcggccacgg acaaggtcat tgccaagggt
     2161 aaacgcatcg ccgcgcacat gctcgaagcc caggagagcg acgtggagtt cgccgacggc
     2221 aatttcgtcg tcagggacag caaccagtcg ctgtccttcg gggaggttgc ctttgcggcg
     2281 tacgtgccgg ccaattaccc cgaggatctc gagccgggac tttccgagaa ggctttctac
     2341 gatcccgaga acttcactta cccggcggga acgcacattg cggaggtgga gatcgaagag
     2401 gagacgggcg tcgtccggct cgtcaacttc gtcgccgtcg acgacttcgg gcacgtcatc
     2461 aatccgatga tcgtcgacgg gcaggttcac ggagggcttg ctcagggtgc cggacaggcg
     2521 ctgctggagc acggcgtgta cgacgactat ggccagctgc agacaggcag ctacatggac
     2581 tactgcatgc cgcgcgcgga cgacgtgccg gacttcgtgg tcgacaccac ggtgacgccg
     2641 tgcacccaca accctctcgg cgtcaagggc tgcggggaag cgggcgccat cggctccccg
     2701 gcggcgatca tgaacgccgt caccgacgcc ctcggcgtca acgacatttc catgccagcc
     2761 acggccgaga aggtgtggcg ggcgctgcgg gcctaggaga agccatgtat cagttcaact
     2821 accaccgccc ggcgtcggta gaggaagccg tcgggctgtt cgaatctatg gacgacggga
     2881 tctacatggc cggggggcag acgctcatcc cgacgatgaa gcagcggctg gccgcgccta
     2941 ccgacgtcat cgacctgtcg ggtatcgaag cgctcagcgg tatcgaggtg agcgccgacc
     3001 gggtcagcat cggcgcgttc accgttcacg atgcggtggc gagctcgccg gagattcgcg
     3061 cgacccttcc tgtgctggct ttcctggctg gccagatcgg cgacggccag gtgcgcaacc
     3121 gaggcacgct ggggggatcc gtcgccaaca gcgacccggc cgccgattac cccgctgcgc
     3181 tgatcggtct gggcgccacg gtgcatactc agacgcgcag catcgaagcg gacgactact
     3241 tcaaagatct cttcgagacg gccctcaacc ccggagagct gattacgcgg atcgactttc
     3301 ccgtgccgac ccgggccgcc taccgcaagt tcccgaaccc tgcttcccgc tacgccgtgg
     3361 tgggcgtgct ggtggccgac ttcggcggca aggtccgcgt gggggtgacc ggtgcgggtc
     3421 cctgcgcctt tcgcgccact gctatcgagg agttgctcag ccgcaatctc gacccgtcgg
     3481 cgctggacgg cgtcgaggtc ccggatgcgg gattcaacaa cgacctgcac gcctccgccg
     3541 agtaccgggc gcacctggtc aaggtgatgg cgaagcgggc cgtggcggac ctgctgagct
     3601 gaagcttcgt accggggcgg ctggctcagc cggcggcgtc ggcggcagcg ctcactcgcc
     3661 ttcttcggcg acgtacagat cctgaaggcg gcgcatgccg ttcagccagc ggtcgtaatc
     3721 ggacgtcttg cgctggatgt aggtggcgac ctccggatgc ggcagcacca ggaagcgctc
     3781 ttcgcgcagc gcttcgatca cgcattcggc ggcctcctcg ggttcgatca tgccgtccac
     3841 gccagccacg cccgggccat cggccgtcat cggggtgcgc acggcctggg ggcagagcac
     3901 ggagactttt atgccgtcgc gagcgtgagt gatcgcgatc cactcggcca gcgcaaccgc
     3961 ggcgtgcttg gtcaccgcgt aggtgaccga gccgatctgt gtcagcaggc ccgcggccga
     4021 ggcggtattg agcagataac cgcctccgcg cgccttcatg cgcggcacca tgtggcgggc
     4081 ggcgtagacg tgggacatgg tgttgacctt ccagatggtg tcccacacct cgtcgggctc
     4141 gtcgagacct ttgccgatcc cgattcccgc gttggaacag aacagatcga tcggcccgaa
     4201 ctcggattca gcgtgctcga tcagcgcgac gaggtctgct tccctgccga cgtctacctg
     4261 catggacgaa ccatctattt cgtcggccac gcggcgggcg ccgtcgccgt cgaggtcggc
     4321 gacgatcacc ttgcctgcgc cctcggcatg aaagcgctgc gccatggcac gcccgatacc
     4381 gctggcgccg ccggtgacga cgacgatctt gcccgagacg tccatctcgt ttcccccgtg
     4441 aaccgcgttg ttttgatgct ctgaacatta gacgttccag aacgttgaat ctatagtcgc
     4501 gaaggattgc gcggtccggg gggcgtgcaa cgtcgccgaa tgacccggag tttggacgca
     4561 gaccatgagt agcaagttga cgacgcggcc cccagccctg acgtggctta tcctggggtc
     4621 gctgctggcg gttctttcgc tgccggcgat ctcgagcgag ccggcgcgac cgctctcggc
     4681 ccaccacgtg atcgtcgacg gcgtgccgct tgcggtatgg cggaaagcgc cggccagcgc
     4741 caagcgcgtc gtgctgctcg ttcacgggcg cacgtggagc gcgcggccca acttcgatct
     4801 gaacgtgccc ggcgaggatc tttctctcat ggacggtctg gtcgaggagg gtattgccac
     4861 gtacgcgctg gacatgcgcg gctacgggga cacgccccgg gatgcctccg gatggctcac
     4921 cccgtcccgg gccgctgcgg acgtcatcgc ggtgctggaa tggattgccc agcgcaaccc
     4981 cggcctcccc gggccgtacc tgttcggata ctcccgaggg gccatggtgg cccaactcgc
     5041 cgctcagcag cagcccgggc ggatctcggg gctcattgtt ttcggctacc ccttgcggca
     5101 ggactcggcg gtgaacccac caggactgac caccgaaccg gcgcggatcg ccaattccgc
     5161 agcggatgcc aggtcggatt tcattgcccc cggggctgcc cgccgggaaa cgatcgatgc
     5221 gttcgtggag agcgcgctca agcacgatcc ggtgcgcgtc gactggcgcg cctatccgga
     5281 gtggctggct ctggacgccg cccgcgtcac cgtgccaacg ctgctcgtgg acgctgagca
     5341 cgacccgctc acggacccca aggttctctc gcgctttttt gcggggctgg ccacggcgga
     5401 caaggcctgg gtgagcatac ccggggcgga tcatgcggcg tttctagaag agccccggga
     5461 ctacttccgt cgggcggtgg ccgcgttcat gctgggagaa cgggcagcgg tagaggtgat
     5521 ctccgcggat ctgctcgact tcgactgacc cttctttggt gcctaccgca tctgcccagg
     5581 gtgggtaggg gccgcctgtc cgggaatggc gcattgagtc tcgcagctga ccgcggttag
     5641 catacccggc caccctgatc cgcaagccca tgtcgacaga cgtcatagtc tacgatgccg
     5701 tcgcgcaaca atggttgcgc ttttgcgatc ccgttgagct gctgatcgcg gaccgcgagg
     5761 atcaggtcct tgacgtcgtc gccgagatcg agagccgcac ggtgggcgac ggcctcttcg
     5821 cggcgggatt tctgacctat gaagccgctc cgggaattgc gcgcgagctg gtgacgcaca
     5881 ggccgcaccg gctccttccg ctcgcatggt tcggtctgtt ccggcgggtt ctgccgagcg
     5941 ccgaccttcc tcttgccccg ggcgactgcc ttggcggatg gtcggcgtcc gtcactcgca
     6001 aggactacga ttcggccatc gcccggattc gagactacat cgcggctggt gacacctatc
     6061 aggtgaacta ctcgtttcgc ctgcgcgctc gtctgaacga ttcccccgcc gccttgttcg
     6121 cgaacatggt gagctctcag ggccctggcc tggctgcttt catcgacacg cccgggttcg
     6181 ccgttgccag cgcatcccac gagctgttct tcgcccgcga cggggacgag ctcgtttccc
     6241 ggccaatgaa gggcaccgcg ccgcgcggtc tgcgggacca aacggatcgg gatcaggccg
     6301 gctggctggc cgcatcggca aagaatcgtg ccgaaaacct gatgatcacc gatatggtcc
     6361 gcaacgacct tggccgcatt gccaggatcg gatcggtggt tgccgacgac ctctttgccc
     6421 tggagcggta ccccaccgtc tggcagatga ccagcacggt aagagcacga tcgaccgcca
     6481 gcctgccgga cgtcatgggc gccctgttcc cggcggcctc catcaccgga gcgcccaaac
     6541 gcagaaccat ggagatcatt cgcgagctcg agcatgagcc gcgcgggatc tacacgggca
     6601 ccattggctt cgtgcgtcct gaggggcgct gccagttcaa cgtggctatt cgcaccgccg
     6661 ccatcgacaa gacgacgtcc gaggcggagt acggtgtggg tggcggcatc gtctgggact
     6721 cccgagccga agacgagtat cgggagtgct tcgcgaaagc gcgcatcgtc accggtgggc
     6781 gccgggaatt cgagctgctc gaaaccctgg cctggcaccc ccgtgacggg tttcgccgtc
     6841 tcgaggagca tctcgagagg ctgcacagcg cggctgacta tttctgctgg tcgctgagca
     6901 ccggaaaggc gcaagccttg ctcgaagcgg ccgcgtcggc gtatcgcgag cccatgcgcg
     6961 tccgcctgct cgccgaccgt tcgggtcacg taaggctaga ggcttcgccg ctgaaacctt
     7021 tgcccgaacc ctaccgtgtg gccctatccg aaccgatgga ggatttgcag agcgagttcg
     7081 tgtaccacaa gacgacgcaa cgttcggtgt atgacaaggc gctcgacgcg gcccgcagcg
     7141 tcctgccgga tctggacgat gtgctgctgg tcaacggcga cgggcaggtg acggagtcga
     7201 ccattgccaa cgtggccgtg gaggtcgacg gccgcctgct gacgcccgag ctcggctgcg
     7261 gcctgcttcc cgggatcgaa cgtcaggtgc tcgtcgccga caaaatcctc gaagaagcgc
     7321 gcgttccggt tagcctgctc gtcgaggcgg agcgcatctg gctgctcaac tccgtccggg
     7381 ggatgtggcc ggcgacgctg gtcgcgaccg agcagggtgg cagcgatctg cgctcggaat
     7441 tcccggggtg aagatggagc gggacacggg actcgaaccc gtcgcctaca gcttgggaag
     7501 ctgtcgctct accagatgag ctagtcccgc cgagaggttg tcgattctgg cacgagcgct
     7561 cgccggtgac catcccgagg tgatggtccc gcaagcggca cggcttcagt cgtcgtcggc
     7621 ccgccgtacc cagcggctgc tggaagccac gtcttcgatg ggctcggcgg cttttttcca
     7681 gccgccgaag ccgggctcga gatgggccac gtcggtaaag cccatgtcct gcagggctct
     7741 gacggccagc acggagcggc cgccgccggc gcagaacacc acggtgcgcc ggtcttcctg
     7801 gaaatagtcc cgaaagtacg ggctagccgg gtcggcccag aactccagca tgcccctggg
     7861 gacgtgaacg gaaccgggta tggtgccaag gtccacgcgc tcctggatct cgcgaatgtc
     7921 gagcagcagc aggtcggggt cgtctgcaag agcctgcttg agctcctcga cgctcaagtt
     7981 ctctatgttc gccttctcgc gctggatggc ttcgaagaca tttttgatcg gcacgtccgt
     8041 gctcccccga atgcgagcag tgagtgtaca ctgagtccct gccggccgcc ccgtcctggt
     8101 ggaactgccg gcaaccgggc gctgtccaac atcagtgcta ccaaccaagg tgaggaacgg
     8161 tcaattgagg cacactctga agtgcgcggt tttcgggtgg ctgctgctgg gcagcttgac
     8221 ggccgctgcg gctttgccgc tggggctgcc gggcggcgag cagcttccca gccttgcgcc
     8281 catgctcgac caggccacgc ccgccgtggt caacattgcc acctacacca cggtacaggt
     8341 ccgcaacccg ctgttggagg atccgttctt ccgccggttc ttcaacgtcc ctgaccagcg
     8401 ccgttaccgg cgaacccaga gtgcgggctc tggcgtggtg atcgatgccg gcaagggctt
     8461 catcgtgacc aacaatcacg tggtcgaacg ggccgatgag atctcggtga cgctggccga
     8521 cggccgttcg atgacggccg agctcgtcgg cagcgatccc caggtcgatc tggcggtgct
     8581 aaaggtggag gcggacgacc tgcagcagat cggcttcgcg gactccggcg aactgcgggt
     8641 gggcgacttc gtcgtggcca tcggcaatcc cttcgggctg cggcagacgg tgacgagcgg
     8701 gatcatcagc gcgctgggtc gcagcggcct gggcatcgag gggtacgagg acttcatcca
     8761 gaccgatgcg tccatcaacc cgggcaattc cggcggcgcc ctcgtggacc tggccgggcg
     8821 tctggtgggg ataaacaccg ccatcctggc gccgagcggc ggaaacgtcg gcatcggctt
     8881 cgccattccc tcgaacatgg tccgggcgat catggatcag ctgatcgagc acggcgaggt
     8941 gcgccgcggc tacctcggtc tagccgttca ggggctgaac gtggatctcg cggaagcgtt
     9001 cggcgtcgac cgccgcgaag gtgtggtcgt ggttgaggtc gagccggaca gcgccgcgga
     9061 gtccggcggg cttcaggccg gtgacatcat cactcgcgtg ggcgatcggg agatcggcaa
     9121 gatcgccgat ttccacagcc aggcggcggt gacgttcgtc ggggaccagg tccggatcga
     9181 ggtgcttcgc gacggccagc cgcagcaggt caccctcaag atcggcgagg acgagctgga
     9241 gaaggtgcgc ggccgccgca tcgatcgccg gttggcggga acgcagttgc agaacttccg
     9301 caaccaggac gaaccgggca tgggtgccgg cgtgctggtg ctcgacatcg acccggacag
     9361 cgacgcttac cggttcgggc tgcgacctgg ggacattctc gtggccgcca accgtcgggc
     9421 catcgtcaac ctcgcagagc tgagggaagt cgccaggctc gacgacggtc agctgctgct
     9481 ccgggtgtac cgctcaggcc agttcgggta cgtcgccatt cgctgaccgg cggcccggcc
     9541 gggcgtttag tcgaacatga tgacgctgcg cgccagctct ccggtctcca gagcggccag
     9601 gccgtcgttg acgtcttcca ggcggatccg gcgggagatc atatcgtcca gatgcaggcg
     9661 gccctgcagg tagaactcca cgaatcgcgg catgtccacg cggaatcggt tggaccccat
     9721 gttcgacccc tgcatcttgc gctccatgag gaactcgggt ccatgcagct cgatgttcgt
     9781 gcccacggga atcatgccga tgacggtcgc ggtgccgccg cgctgcagga tcttgaaaga
     9841 ctgctcggcc gtctgcttga gaccaatggc ctcgaaggag tagtgcacgc cgccaccggt
     9901 catctctctc accgcctcga tgacgtcgct ctccctggca ttcacgacgt cggtggcgcc
     9961 gaatttgcgc gccagatcga gcttgctttc cacggtgtcg atggcaatga tccgggcggc
    10021 gccggcgatg gcggccccgt tgatggcgga gagcccgatg ccgccacagc cgatcacggc
    10081 gaccgtgctg cccggctcca ccttggcggt gtgaatgacg gccccgacgc ccgtcgtcac
    10141 gccacagccg atgagggcgg cccgatccag aggcatgtcc tcccggattt tcgcaatggc
    10201 gtgctcgtgg acgagcatgt actcggcgaa cgaggacagg ttcaggaact gccagatgtt
    10261 ctcgtcgccc ttgttgaggc gctgcggctg gtccatgggc cgctgcagct cgggctcctg
    10321 gcacagcgac atgtggccgg tcaggcagta ctcgcagtga ccgcagaacg ccgaaagaca
    10381 cgttatcaca tgatccccgg gcttgacgta gttgacgtcg ctgcccacgg cctcgacgat
    10441 gccggcggac tcatggccga gcacggccgg caggggatat ggatacgagc cgttctggaa
    10501 gtgcagatcc gaatggcaca cgccggccgc caccgtgcgc accagcacct cgcgtgggcc
    10561 cgggtcgccg tgctggatat cttcgatttc cagtggcttg ttgatctcgc gcaaaacggc
    10621 ggctttcatg ggcctctccc tgtacgtgtc cgacgtttga gtgtgccaaa cgcatggtcc
    10681 gattactacc atctgccgcc ggtcccggcg cccgatctcc cttactaccg ggggatagca
    10741 gaggttagta ttggccctgg gggatgcctg ctaggacacc ggaaaaacga tggtattggt
    10801 tggtcttgga tccaatcggg gcgacagcgt cggtatcgtc cgcgccgcca tgggcaggct
    10861 tcgtgggt
//
LOCUS       DBNHLM010000207         2791 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_149619, whole genome shotgun sequence.
ACCESSION   DBNHLM010000207 DBNHLM010000000
VERSION     DBNHLM010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_149619"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..739
                     /locus_tag="AC16QF_09415"
     CDS             74..739
                     /locus_tag="AC16QF_09415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR6448816.1"
                     /translation="MIRATAATIVQVLLFGVCLFGAAGSLSWPMAWAVLGIYLLPKAV
                     AFAFLDPELIRERATPGPGVDRGDVVLASLGCLALYPGTFVVAGLDAVRFGPALPMAQ
                     SVQVAALLVYALGYGFAYWAVYSNPFFATFVRIQEDRDHSVASSGPYALIRHPGYAGV
                     LLAHLALPFALESVWALLPAALGMLFFVARTAREDRTLQERLSGYREYQLRVRWRLLP
                     GVW"
     gene            1002..1442
                     /locus_tag="AC16QF_09420"
     CDS             1002..1442
                     /locus_tag="AC16QF_09420"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR6448817.1"
                     /translation="MRLPTLDDAIEASHRALQAIIRGDVEPFMALYSDANDITIGNPF
                     GPFAAGHDATRAAGTRAASNYTDGEILAFERIATHTTDTLACVVEVERFSVRLAKAAE
                     LATVALRVTSLYRREAHGWRLVHRHADPITSVRPAQSVASSERG"
     gene            1596..2075
                     /locus_tag="AC16QF_09425"
     CDS             1596..2075
                     /locus_tag="AC16QF_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014136.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6448818.1"
                     /translation="MSESAPTINLADAPMKRGGNGGKFVHNSCRLGPKIGLKTLGCGV
                     YVLPPGKRAFPYHAHSLIEEMFFILEGVGTLRHEGREYPIRAGDLIASPVGKAHQIVN
                     SSDADLKYLAISSNVLADVVIYPDSNKVQAVSGAFPKSLWHVTKLSDATDYYAGEEA"
     gene            2180..>2791
                     /locus_tag="AC16QF_09430"
     CDS             2180..>2791
                     /locus_tag="AC16QF_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448819.1"
                     /translation="MFTDDEFFGSPERIALSRRSANLWSLLRDNPRYAYYGRLVALSD
                     PGRDAAEILSAMARLQGGAVSYFYPARTAASLFAQLEDSGLHTDRHEHFWGGETALAA
                     SRRALADLALPADLSLSVIDEGTPRELVRAVAELCQSCDVMPVPGSIMRGRARKGICL
                     VAIDLTGQPVATASSYMIHHPSSPRAKDAFWGMLATREDRRGER"
BASE COUNT          496 a          916 c          853 g          526 t
ORIGIN      
        1 gccccgtggg ctaccctccc ggtcagcggc gctcgcagct gagcaccgat ccgttaggcg
       61 gcagggtggc actttgattc gggctaccgc agcgacgatc gtccaggtac tcctcttcgg
      121 agtgtgcctg ttcggcgccg ccggcagcct ctcatggccc atggcgtggg cagttctagg
      181 gatctacctt ctgcccaagg cggtcgcctt cgcgtttcta gaccctgagc tcatccggga
      241 gcgggcgact cccgggcccg gcgtcgatcg cggcgacgtg gtgctcgcat cgctcggctg
      301 cctggctctg tacccaggta cgttcgtcgt ggctggcctc gatgccgtcc gcttcggccc
      361 tgccctgccg atggcgcaat ccgttcaggt ggcggcgctt ctcgtctatg cgttgggcta
      421 tggcttcgcg tactgggccg tctattccaa cccattcttc gcgaccttcg tgcggatcca
      481 ggaggatcgc gatcactcgg tcgcctcctc ggggccctat gctctgattc ggcatcccgg
      541 ctatgcgggc gtgctgctcg ctcacctggc ccttccattc gcactcgagt ccgtctgggc
      601 cctgctgccc gcggccctgg gcatgctctt tttcgtcgcg cgaacagcgc gcgaagaccg
      661 gacgctccag gagcgtctca gcggctatcg cgagtatcag ctgcgggttc gctggcgtct
      721 gttgccgggc gtttggtagc cgtacgcctg cggcccaaca aggcgctgca gctgacgagc
      781 gcccggtccc ttaggtcgcg gcaaacggcc gggggccctt tccgcccgga tagcctcccc
      841 gctcggcttg gccacgcctt cgcagtggcg agccggctcg acgacagtgg acgttatgac
      901 gggcatgaga caagatcacc ggcattggat gccgcaagac aatcacttgg cagctaagct
      961 ggtttgtcca gccaggcggc aattcacaca tgggagcggg catgcgactg ccaacgcttg
     1021 acgacgccat cgaggcgagc caccgcgcgc ttcaggccat catccgaggc gacgtcgaac
     1081 cgtttatggc cctgtactcg gatgcgaacg acatcacgat cggcaatccc ttcggaccgt
     1141 ttgccgctgg gcacgacgcc accagagcag ccggtacccg agctgccagc aactacacgg
     1201 acggcgagat cctggcgttc gaacgtattg ccacccacac caccgacacg ttggcctgcg
     1261 tcgttgaggt cgaacggttt tcggtacggc tggcaaaggc ggccgagctc gctacggtcg
     1321 cgcttcgggt caccagcctg taccgccgcg aagcccacgg ctggagactc gtccaccgcc
     1381 atgccgaccc catcaccagc gtccgaccgg cacaatccgt tgccagttca gagcgaggtt
     1441 gacttggcgg aaggaatgtc cgtcgcttca gcacaggcgc cgcacttcac cggcagagta
     1501 catccgcgca gcggcctgac atccggccgc cgcaagtgag ctggcgcgtt gggcctcaag
     1561 cgcaccgagt agccgattac aaggagggag aaccgatgtc cgaaagcgca ccaaccatca
     1621 atctcgcgga tgcgccgatg aagcgaggcg gcaacggggg caagttcgtc cacaacagtt
     1681 gccgacttgg accgaagatc ggattgaaga cgcttggttg cggtgtctac gtactgccgc
     1741 ccggtaaacg ggcgtttccc taccatgccc actcacttat cgaagagatg ttcttcatcc
     1801 tagaaggcgt gggcacactg cgccacgaag ggcgggaata cccgatacgg gcgggagacc
     1861 tgattgcctc accggtggga aaggcccatc aaatcgtcaa ctcctctgac gctgacctca
     1921 agtacctcgc gatctcaagt aacgttcttg ccgacgtcgt tatctacccg gactccaaca
     1981 aagtccaggc ggtctcgggc gcctttccca agagcctttg gcatgtgacg aaactcagcg
     2041 atgcgacgga ctactatgcg ggcgaagagg cctaacagcg cagcccaaaa acgaaggcgc
     2101 gcctgggaca cgacttcagc accgctggcc gcagccgcct ccgtgccgct ccattgatct
     2161 gggcgaatcg cgaggccgca tgttcaccga cgatgaattc tttggctctc ccgagagaat
     2221 tgccctctcc agacgcagcg ccaatctgtg gtcgctgctt cgggacaacc cgcgctacgc
     2281 atattatggc cgcctggttg ccctgagtga tccgggccga gacgcagccg agatcctctc
     2341 cgcaatggcc agactgcaag gtggcgcggt ctcctacttc tatcccgccc gcaccgccgc
     2401 gagcttgttc gctcaacttg aagacagcgg gctgcatacg gatcggcatg agcacttctg
     2461 gggtggagaa acggctcttg cggcgagccg cagggctctc gcggaccttg cgctccctgc
     2521 cgatctcagc ctatcggtga tcgacgaagg cacgcctcgc gaattggtga gagcggttgc
     2581 tgagctttgt caatcctgcg acgtcatgcc agttccgggt tcgatcatga ggggacgggc
     2641 ccgaaagggc atttgcctcg tcgcgatcga tctaacggga caacccgtcg caacggcttc
     2701 gtcgtacatg atccaccatc catcgagtcc tcgtgcaaag gacgcatttt ggggaatgct
     2761 ggcgacgcgc gaagacaggc gcggcgaaag a
//
LOCUS       DBNHLM010000208        31352 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1645763, whole genome shotgun sequence.
ACCESSION   DBNHLM010000208 DBNHLM010000000
VERSION     DBNHLM010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 31352)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 31352)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31352
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1645763"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..112
                     /locus_tag="AC16QF_09435"
     CDS             <1..112
                     /locus_tag="AC16QF_09435"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HIR6448820.1"
                     /translation="MRGVEKQNSLMKTSAMLGAFRENPSTRTEFLNLLKD"
     gene            complement(137..310)
                     /locus_tag="AC16QF_09440"
                     /pseudo
     CDS             complement(137..310)
                     /locus_tag="AC16QF_09440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            439..1710
                     /locus_tag="AC16QF_09445"
     CDS             439..1710
                     /locus_tag="AC16QF_09445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6448821.1"
                     /translation="MNFEIPEDIAELLDRLDDFIEQVIKPLEQQDDNIRFFDHRREDA
                     RTDWDRGGLPNEEWEQLLFKVKRLADEAGFYRYPFPKEYGGMNGNNLGMAIIREHLAV
                     KGLGLHNDLQNEHSIVGNQVGLLLMLEYGTDEQKAEWVDDLANGVRGFAFGITEPNHG
                     SDATHMETHAERDGDEWVINGEKTWNTGIHKAKYDMVMARTSGKPGDGDGITAFLTPM
                     EADGVKIEEMLWTFNMPTDHGRVSFTNVRVPHVSIFGGEGRGLQVVQHFFNENRIRQA
                     ASSLGAAQFCINESVRYAQERKPFGKALATNQGIQFPLVELQTQCEMLRALIHKTAWE
                     MDTYGAFSVSDKVSMCNYWSNRLCCESADRAMQVHGGLGYSRHKPFEHIYRHHRRYRI
                     TEGAEEIQMRRVAGYMFGFMSQRAPKGVRES"
     gene            1713..3140
                     /locus_tag="AC16QF_09450"
     CDS             1713..3140
                     /locus_tag="AC16QF_09450"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6448822.1"
                     /translation="MDHAEFEARLGKVLALRIGRVRSLRSAERLSGGASQETYRLTVD
                     TADGEELMAMRRAPGGGLDVEVAPPTLATEALLMRSARAAGVPEPEVYYVLEPEDGLG
                     EGFVMEWLEGEALGARIVRARELDGVRPQLAYECGRVMAKIHAIDLDRAGLRERLETL
                     TPEELLHRSWDRYKDFPTPQPMIDFTARWLLDNMPATFDMALVHNDFRNGNFMVDANG
                     IVAVLDWEVAHIGDPMRDLGWICTNSWRFGRSDRPVGGFGEYADLFRGYEDESGVAVD
                     PERVKFWEVMGSFWWAVGCLGMAEHYRTGPDKTVERPAIGRRSSECQVDCVNLIIPGD
                     VELVAGTDLEDCTDMPRVDELLVSVRDYLRADVMAATSGRTNFLARVAGNSLDIVLRE
                     LATGAEARFREQVRLEALLGRSGDLDALRWELVHRLRSGEMALDDVALTDHLRTTVVN
                     QIAIDQPRYSGFKTALARAGIEVPE"
     gene            3137..3625
                     /locus_tag="AC16QF_09455"
     CDS             3137..3625
                     /locus_tag="AC16QF_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448823.1"
                     /translation="MNIRTDAIVRLRDHLLSSDLGAAGQVPDEQPGDAVRLRVEPFAE
                     TMFLVMLADAEQAVSEHDALVVAVDMLTDGLMGREAVDALLAEFAERVRREGREDCVA
                     RIGARLSADRDDREMAFTLAAVIALADDRVAVEENEVLDLMRTYYGISDRRMEAILGT
                     LV"
     gene            complement(3622..4488)
                     /locus_tag="AC16QF_09460"
     CDS             complement(3622..4488)
                     /locus_tag="AC16QF_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6448824.1"
                     /translation="MTGLRLGVVGDLHTHWDDIDVAQFNATRYDLLFFTGDLGGGSVE
                     SSLRVARTISRLDVETLVMPGNNDTGDIHQLAAELSHRSGLKRLTGIRQGAGDAIKPI
                     RLCGYSSHRYRTPAADVTLIAARPHSMGGPTLTFADHMSATYGISSLEASAKRLKELV
                     DAAETADLVFLSHNGPTGLGGEPADMWGCDFKPDGGDWGDPDLREAIAHAVATGRRVL
                     AVVAGHMHLKTKCGQERPWRREHDGILYVNAARVPRIYSGSDDVMRHHVSLHIHEQGI
                     EIDELEVPQGLG"
     gene            complement(4485..5717)
                     /locus_tag="AC16QF_09465"
     CDS             complement(4485..5717)
                     /locus_tag="AC16QF_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6448825.1"
                     /translation="MSNETRCCIVGAGPAGMFLALLLARRGVTVTVLELHRDLDRDFR
                     GDTVHASTLEVLDQIGLADRLLALPHAKMRQVTLHTAARTLELVDFSRLKTRFPYVMV
                     MPQVVFLNYLHEQAKSYPGFRCVLGAQVQQLVEVDGRVSGVRYRRDGEEHELRADLVI
                     AADGRFSRLRKLAGLEAESLSPPMDVCWFRLPREASDGHEAGGFFVGQGRMLIALPRS
                     DEWQIGYVFPKGDFKTLRESGIQAFRDSIAATAPWLAGRTDVIHGWERVHLLTVSSDR
                     LPTWHRPGLLCIGDAAHVMSPVGGVGINAAIGDAVEAANVLTRPLLDAVAPGEAALAE
                     VQRRREVPTRIIQGLQARIQDTVVSRALRDEEFHLPLPARLILATPGLRNLPPRIFAQ
                     GIKPLRLEALPRTEHQRT"
     gene            5866..6558
                     /locus_tag="AC16QF_09470"
     CDS             5866..6558
                     /locus_tag="AC16QF_09470"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007992649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR6448826.1"
                     /translation="MGATSALHAAEPEELLKRRLMAEIRGDAKRTADYTGRSALDERV
                     MAALEAVPRHEFVEAWSPGAAWENVPLPIGHGQTISQPFIVALMTDFVEPHPTHRVLE
                     IGTGSGYQAAVLAGLVGEVYTVEIIPALAQTATERLHRLGYDNVFVRVGDGFYGWPEK
                     GPFDGIVVTAVAPEIPAPLLEQLRPGGRMILPVGEVHGGQNLVLVSKDADGAVATRDV
                     LPVMFVPLTGDH"
     gene            complement(6570..7379)
                     /locus_tag="AC16QF_09475"
     CDS             complement(6570..7379)
                     /locus_tag="AC16QF_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007980046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6448827.1"
                     /translation="MTERYQGYQRLEFDYPHPRILRISFNRPERYNALDVVGHNEITY
                     VWREVDADPDIDCVILTGKGKAFSAGGDFEMIEEIIADFDTRAQTWKEARDLVYNIIN
                     CGKPIISAINGPAVGAGLVAGLLADISIVAESVSLTDGHTRLGVAAGDHAAIIWPLLC
                     GMAKAKYYLLTCDRLSGAEAERIGLVSMCVPDEELESRALSVAVQLANGAPNAIRWTK
                     YALNNWLRLAGPTFDASTALEMLGFTSGEAREGVSSLKEKRDPEFKKGSRI"
     gene            7537..7869
                     /locus_tag="AC16QF_09480"
     CDS             7537..7869
                     /locus_tag="AC16QF_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448828.1"
                     /translation="MRLLFICLVALAPVAAAHTPVPVHGCAFPERPPDDVDEMTWDRF
                     LEGVERYRACISDFVRSNYAASDAHRDAANAATLDWNAFVRDSLNVPEDFPWEPEDSD
                     DQAEPDNP"
     gene            7950..9410
                     /locus_tag="AC16QF_09485"
     CDS             7950..9410
                     /locus_tag="AC16QF_09485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT/MGAT family O-acyltransferase"
                     /protein_id="HIR6448829.1"
                     /translation="MQQLTGLDASFLYLETPKTPMHIGGVMIYDQRTVKGGKLRFKDI
                     LDHFESRLHLARTFRQKLVHVPFNLDHPYWIEDRDFDLEFHVRHIRLPEPGDWRQLCI
                     QVARLHSRPLDLTKPLWEFTIIEGLDDIEGLPRGSFAVVTKIHHACIDGVSGVDMVEA
                     IHDVEPYVDRSPGPVTPWKGEQEPSAIELMVRAQVHNMTQPYRLAEVLARTVPALGRL
                     GQRATGGDAERASLAPPRTRFNHVVSAHRVIEGRRFDLAEIRAIKDTVDGATVNDVVL
                     AVCGGALRRYLLAKHELPGVPMIAMAPISVRSSDEKGAMGNRISAMTISLGTHIEDPI
                     GRLSAIHEAAVASKGMSNAVGAKLLTDYSQFIPSTTAAMAARLYVQLGLSERVNIPFN
                     CVVTNVPGPQFPLYSAGAKLLAQYGLGPIFDGMGLIFPVFSYNGKLFLSVTSCREMMP
                     DPEFFSECMQAAFDELKAAALVIPEAADASLAGGTP"
     gene            complement(9431..10105)
                     /locus_tag="AC16QF_09490"
     CDS             complement(9431..10105)
                     /locus_tag="AC16QF_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448830.1"
                     /translation="MGQTDSTSRSDVIDQLVASYLKIPLKIYWRETRGLPFPDTFIDA
                     RLEFAGMTTSWLRLDSVIWRAGEVRFVPGFPARLYVRSPSVEFVVGQAELDRWQSRFS
                     LPYRLELTESGITVHTELAGFPLAEFETGLTVVDGWFVLEPKRATVLGVPGYVSSLFR
                     GYLPVPPLSGEIRLAGIEHAAGILRVRFDIDDFDEEVTPGMLSRLQKRLFSFNSPLGP
                     TRRDDA"
     gene            10160..11884
                     /locus_tag="AC16QF_09495"
     CDS             10160..11884
                     /locus_tag="AC16QF_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448831.1"
                     /translation="MSGQTNSVGGEKVPLVIGVTGHRDLLAEELPVIRRRVQDLFAEL
                     QRQFPDLPILVMTPLAEGADRLVAEVARDMGMPVEVLMPMPRELYQADFSGESLVEFD
                     EMLRLGEIVELPLVPGNTEKTVAAGGRPRDLQYAQLGAYLAAHSHVLLAIWDGKPPEA
                     VGGTAHVVQFHQHDVIELLAEGQRRSPIDFAEDESDLVFHIACSRLADGAPREPLQPG
                     QAEWLTRDDVQPRTPQMPPRYRVVFERLVEFNRDLAEPIPAGSLDPLVTGDDADHCGP
                     GAEDIERLYMVADFLARHYKKYRRRLQTATYALAVCAIMSFIVWADLPDQEIMIYPYL
                     GFIGLVIVLVYLERRKGWQRRSLEYRVLAEGLRVQFWWSMAGVVMENPSRFSHDSFLR
                     QQDLELGWIRNVMRFAGRRADANIGNEDGADTDLVARVWVADQKAYYQSKSQERNRGS
                     QLTSALVFATFGVGVTVAIIFAVFQAEIAAPWSNMLVALMGLLPFLAAVRQSYANSTA
                     EREDFVQFGYMYRIFFNADRLLNSARTVDGRRDILRALGEAALEEHGQWMLRQRERPA
                     AGRVLQGG"
     gene            11884..13035
                     /gene="argE"
                     /locus_tag="AC16QF_09500"
     CDS             11884..13035
                     /gene="argE"
                     /locus_tag="AC16QF_09500"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF005710.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HIR6448832.1"
                     /translation="MARRFTPREMLEKLIAFPTVSRDSNLELIEFVANYLDDHRVQAR
                     LVASGDGRKANLYATVGPEIEGGVVLSGHTDVVPVDGQNWDSDPFLLHEVDGRLVGRG
                     TCDMKGFLAIALALVPDMGALRYPIHLAFSYDEEVGCLGAPDLIREMTANVPRPKAVI
                     VGEPTSMQVVGAHKSMFIFDTVVRGHEAHSSQQHRGVPAVMVAGRLIGWLAERQRQNA
                     RVADPDSDFVPGYTTLHCGTVRGGTAHNITAAECRFATDIRSLPGEPAESYFREFEQF
                     ARQTLEPEMRAVHPGSGIDFEIRAQVPAFQATGDDPAVALASQLTGQNGLESVPYGAE
                     AGQFQEAGHSVVMCGPGSIDQAHQPNEFLTLEQLQAGATFARRLIEQLS"
     gene            13238..13882
                     /locus_tag="AC16QF_09505"
     CDS             13238..13882
                     /locus_tag="AC16QF_09505"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR6448833.1"
                     /translation="MTARRPSKGFSLIELMVVVGILAVLAAFAMPAYLDYLESANTSK
                     VLSHYEDGTRYLRTEILRLNTSVVMGKETLESIDANFTSAFWVDKLNQEGGRAPGGGP
                     AYGASVDHGMGRVGVVVAGSLLDAPPSYRLEVTRPQYGGFAQYSVMTAIISWSGGGGG
                     TSFGSQADPTPGQPGGLDGSGPGQGAGSADNPGQGKDQGQDKGKGQGQGQGQNV"
     gene            complement(13890..15146)
                     /locus_tag="AC16QF_09510"
     CDS             complement(13890..15146)
                     /locus_tag="AC16QF_09510"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR6448834.1"
                     /translation="MRRSRILILLSTVAAGCGEPGVSPDLEHARALSRSELIVDTHID
                     LPYRLYRRHEDVAEPTSGEFDYPRALAGGLDAAFMSIYVPAAVDAEGRATEMADRLID
                     LVEGIARDAPDKFAVATCTRDVEAIRASGRIALPMGMENGGPIAGDLGKLDHFMGRGV
                     RYITLAHSKSNHISDSSYDENEPWEGLSPFGRDLVTAMNQRGMMIDVSHLSDRAFWQV
                     LELSSAPVVATHSSLRHFVPGFHRNMSDEMVAALGRNGGVVQINFGSSFVTAEARAYS
                     DAMTAAVKAFAETEEPRYDDPAVRAFAASYRAEHPYPYAELEDVLNHVDRAVALAGIE
                     HVGLGSDYDGVGDTLPVGLKDVSSYPNLIAGLANRGYDDHSISLILGGNLMRVWREVE
                     AVAAAQGFPTACRHDAPARSALSQRL"
     gene            15241..16761
                     /locus_tag="AC16QF_09515"
     CDS             15241..16761
                     /locus_tag="AC16QF_09515"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011335679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006091 - generation of precursor
                     metabolites and energy [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HIR6448835.1"
                     /translation="MTIIREDDLIESVADALQFISYYHPLDFIRAMRSAYEKEESPAA
                     KAALTQVLVNSRMCAIGKRPICQDTGIVNVFLEVGMEVSWSGKRSVEEMINEGVRRAY
                     MHPDNVLRASVLTDPDGARSNSRDNTPAVIHTRIVPGDKLSVEVAAKGGGSEAKAKFA
                     MLNPSDSIVDWVLDVVPTMGAGWCPPGLLGVGIGGTPEKAMLLAKEALMEPIDIHDLK
                     ARGPANHLEELRLELFEKVNALGIGAQGLGGLTTVLDVKVKDYPTHAANKPVAVIPNC
                     TATRHVHFTLDGSGPAVFEAPDLDEWPDIAFELGDDVRRVDMDTLTPDDVRNWKAGDT
                     LLLTGKMLTGRDAAHKRLADLIERGEPLPVDFTNRIIYYVGPVDPVRDEIVGPAGPTT
                     ATRMDKFTGMMLEKTGLIGMIGKAERGPAGVDAIREHGAVYMIAVGGAAYLVAKAITR
                     STPLAFEDMGMEAIYEFEVKDMPVTVAVDSTGASVHREGPRLWSQKIADRIGAAQL"
     gene            16796..17299
                     /locus_tag="AC16QF_09520"
     CDS             16796..17299
                     /locus_tag="AC16QF_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448836.1"
                     /translation="MATRRRTLAMGLALLAGGYLGPLARHARAQSLEQAMEESDLIYL
                     TPLKSDGSESRCRAEVWFARHEADLFVVTSSDAWRARAVERGLTRTRVWVGDVGVWSG
                     SGGSYRTLPQIEADASIVAEADVHDAVLDVMGDKYRMSWLVWGPRFRNGLADGSRVML
                     RYRPAAA"
     gene            complement(17335..18270)
                     /locus_tag="AC16QF_09525"
     CDS             complement(17335..18270)
                     /locus_tag="AC16QF_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR6448837.1"
                     /translation="MNSSASDRVIWRNGEFVPWADATVHVLAQSLQRGTLAFDFMSVH
                     ETADGPAIFRLEAHIERLLQSCRLEELPILYPAEALMEACAETVRRNPGCKSVKISAL
                     IASIEVELIPQDPAVAVFIAAYDSARDIMAKHQTPYPTRETLALKVEREISNRRHDIL
                     PPQAKVAANYTSPTIAKRKARAEGFDDVLLLTERGTVAEAPTSNIFVVRDGGLATPPE
                     NDVLLGITRASIIEIAGFLNLPCEEQELELTQLYAANEAFLTNTSTGVWPIERVDDVV
                     FGDGAPGPVTKRLRAALRKISSGEEPTFRHWLHYV"
     gene            complement(18296..19291)
                     /gene="dgcA"
                     /locus_tag="AC16QF_09530"
     CDS             complement(18296..19291)
                     /gene="dgcA"
                     /locus_tag="AC16QF_09530"
                     /inference="COORDINATES: protein motif:HMM:NF042940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-D-Glu racemase DgcA"
                     /protein_id="HIR6448838.1"
                     /translation="MDVFAHIESWPIAGTFTIARGSKTVAEVVVVTVREGDLTGMGEC
                     LPYPRYGQSPEGTLEEIRVATAGHAILDRQRLTELMPAGPARNALDCALWDLEARKSG
                     QPVWRLAGLAEPRPVVTALTIVLGEPGQMREAAERHSRRSLLKIKLGAGAGRDIERLR
                     AVRSGAPDARLIVDANEGWTLGELEQVAPVAADLGVEMIEQPLPVSEDEALDAFRCPV
                     PLAADESLRAGEDLDRLAGRYQVANIKLDKTGGLTEALILQKKAETLGMDVMVGCMVA
                     TSLSMAPALLLAQTARFVDLDGPLLLVRDRQPGLDYHDSLVAFPQQAVWGAGEHG"
     gene            complement(19308..20351)
                     /gene="dgcN"
                     /locus_tag="AC16QF_09535"
     CDS             complement(19308..20351)
                     /gene="dgcN"
                     /locus_tag="AC16QF_09535"
                     /inference="COORDINATES: protein motif:HMM:NF041892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase DgcN"
                     /protein_id="HIR6448839.1"
                     /translation="MRSPNDMASAGTRIEAPYLIFLGSAHDQLAAKTGQGIVDWRPQN
                     CIGQLRLPECQADLGLPDLSVAEAAAAGARTLVIGTVSPGGSLPEKWLPILHAALANG
                     LDIASGMHDRLDSFPEIRDAAARHDRRLVDVRHSTVRFPTGSGRKRSGRRLLTVGTDC
                     SVGKKYAALALEREMRSRGMDAEFRATGQTGILIAGSGVAIDAVTADFISGAAECLTP
                     DAERGHWDVVEGQGSIFHPSFAGVSLGLLHGTQPDAVVVCHEPTRRTMRNVSTLMPRI
                     GEVIEQTLVHGRTTNPDVVCIGVAVNTAALDEGEARELLTNYAAQLGLPVTDPIRFGA
                     GLLVDELVKNFPD"
     gene            complement(20387..21403)
                     /locus_tag="AC16QF_09540"
     CDS             complement(20387..21403)
                     /locus_tag="AC16QF_09540"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6448840.1"
                     /translation="MDTAEPGSQAWLDLVEEPIIDPDRVIVDPHHHLWDQPSAFGRYT
                     LEDLWRDTGSGHRIAKTVFLECGASYRESGPEHLRCVGETEYVTQAARASSAQSDGNA
                     VIAGIVARADLRLGDALDEVLDAHVEVAGDLFKGIRHSGAHAREPEILRIAGRAPAQL
                     YANDAFRRGVRRLGELRLTYDTWHYHYQNPQFLALARAVPDTIMVLDHFGTPLGVGPY
                     ADRREEIFRQWQDDMSRLADCPNVVAKLGGLAMPDNGFGWHAAARPPTSEEFAEAQRP
                     YYRHMIDVFGADRCMFESNFPVDRMSLSYAVLWNGMKRIAADFSEAEQDALFSGTATR
                     VYRL"
     gene            complement(21535..24729)
                     /locus_tag="AC16QF_09545"
     CDS             complement(21535..24729)
                     /locus_tag="AC16QF_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6448841.1"
                     /translation="MYTAISWFTRNPVAANLLMFVLLIGGCLALFNVHQEEFPSMDVR
                     VVNVTVPYLGAAPEEVEEGVCIRVEEALEGTEGIEKLLSAAREGSCSVQAMLYEDANQ
                     TEALNEIKSQVDGINTFPLETEKPIVSKLTITSDVLQIAVYGDTDERTLKELGKELRD
                     ELSMIDGISKIALNYVRPYEISIEISEDTLRRYGITLEQVARVIRNTSMDMPGGTIKT
                     KGGEILLRAKGQAYWGEEFEDIVVVTRNDGTKVMLSEIAEIRDEFEEGDMRARFNGYP
                     AAVVKVWQVGKEDALEMAADIKAYLTRYEKTLPAGLFVKVWNDESEQLKARLDTLNGM
                     ALSGLGLVVIILAMFLKFRLAMWVAAGIPIALLGTLAVFPYADISMSAMTVMAFILVL
                     GILVDDAIVVGERVYGHEQMGKPPVQAAIDGTWEVSIPVIFGVLTTMAAFLPLLLSTG
                     RMAGFFSVVGWVVIIALVFSIVESQLILPSHLAHRNNDEASNSFSRRWNRIQGRLARW
                     LENIANYRYEPFLRKAIEYRYITASVGLGLLIMSVALIASGRVVFAFFPGIEGDRIYA
                     TLEMPEGISVEATGEAVDIIEAAGERLGKALDAELNQTATIRNVFTSIGMPADRQNGP
                     SRGGSAGRSHFAEVVLELAPGEERNYLSAKEIAHRWRKLVGNIPDAVKLSFTADAFSV
                     GEAINYELSAGDPDVIRAAATDLRAELSRYVGVYDIGDSFRAGKQEIKLSLLPEARNL
                     GLTLADLASQVRSAFYGYEAQRIQRGQDDVRVMVRFPEAERTSIGNLEDMYIRTPQGT
                     EVPFYSVAKFELGRGYSTIRRVNGRRVINVTAEVDRSVVSPEEIGSSISSKVLPKLRA
                     KYPSLKVGVSGEQEERTKAFTGLAQAAMLALIVIYALLAIPLRSYLQPLVIMSVIPFG
                     AVGAIVGHWLMGYQLMFFSALGMVALSGVVVNASLVLVDYTNRRRREGMPIAEALRSA
                     GVVRFRPILLTSVTTFVGLIPLMFSDTPATAFFIPMAISLAFGVLFATAITLLLVPAL
                     YHLAEDVSGWDPVAQGVEEHRSNIRSTGKLQPGG"
     gene            complement(24734..25888)
                     /locus_tag="AC16QF_09550"
     CDS             complement(24734..25888)
                     /locus_tag="AC16QF_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6448842.1"
                     /translation="MMKKLLVPGLVILASLFGAVTLMATAPELEPNAIEPLATTVRVV
                     EVIPQPVRLTVHSQGTVVPNMESELIPEVSGRVEWISPSLVNGGYFEQGDVLLRLEAT
                     DYRSALTRAEAHLDRAAAEFEHAQYEYQRLKSLEVRQLASKSQMENSLRAFRVAEASL
                     QEARAAMEQAMRDLERTALTAPFDGLVRREAVDVGQFVSRGSPVATVYASDQAEVRLP
                     IADRQLAFLNLPVDHRGELPAEQQPRVTLLAEYAGRSLEWQGTIVRTEAQIDTASRMV
                     HVVARISNVEQPVPLNVGLFVNAEIEGLLADNIVVLPRTALRDGDRVLVVDGGNNLRY
                     RDIEPLRLYKDEVLIKAGLAAGERVCVSPLQTAVDGMPVQPVTENAPTAG"
     gene            complement(25885..26613)
                     /locus_tag="AC16QF_09555"
     CDS             complement(25885..26613)
                     /locus_tag="AC16QF_09555"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR6448843.1"
                     /translation="MAKHEEIAESLTRDILSGQYRTGERLPSERDLSVRFEANRGAVR
                     EAMKKLEQLGIANIQPGGARVAPLEEASLDVIGHMLALGDLPDSKLVIHIIEVISALV
                     RLAVESALNTATDEDIERLREHNRPLFEEDLDDLAHMEARLELMGAIMSASDNLVCQL
                     IARSLLAQLTPRTEPLKAFAQFDTEAHADNARRLDEALAKRDADALRHVFTESTALTR
                     ESVLRSFEAYDAANRAPLTGVAAS"
     gene            complement(26777..27787)
                     /locus_tag="AC16QF_09560"
     CDS             complement(26777..27787)
                     /locus_tag="AC16QF_09560"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIR6448844.1"
                     /translation="MAELTFKDYATDQEVRWCPGCGDYSILKAVQRALADAGAAKEQT
                     VFVSGIGCSSRLPYYVDTYGFHTIHGRAPAVATGVKLANPDLDVWVITGDGDALSIGS
                     NHLMHALRRNVDLNILLFNNEIYGLTKGQYSPTSRIGTRSPSSPKGSVDSPVQPGLFA
                     VGAGATFIARAVDVDQKVLPGILNAAREHKGAALVEILQNCIVYNKDVFEDVVAKKTA
                     ADTQVVVEHGHPLVFGKERDKGLRLNPQTLALEAVTLGENGVSEADLVVHDETNTALA
                     HMLLSLAPPNPVAMGIIHRRRAPAFDAAYWSSKPTGRSARVAELLRHGNVLDHRRSPQ
                     GR"
     gene            complement(27791..29644)
                     /locus_tag="AC16QF_09565"
     CDS             complement(27791..29644)
                     /locus_tag="AC16QF_09565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR6448845.1"
                     /translation="MVEEKTSYVIRFAGDSGDGIQLQGQQFTTASALAGHDLATMPDF
                     PAEIRAPAGTRYGVSAFQIQFGGDRVTTPGDAPDVLIAFNPAALVVNLSHLRPGALVV
                     VDRQAFTDQRLKRADLTSNPLDDGTLDGFELLDIDISDLTHQAVDALGLGAKDSLRCK
                     NFWALGLVLWMFDQKRDATVSWIEAKFAGNEQVRSANLAALNAGHAFGETAELGPKLR
                     QHAAPEASLASAEYRTLTGAEALALGLVAAGELADTELMFCSYPITPASSLLHQLTGL
                     EDAGVATFQAEDEIAAICSAIGASYAGKLGVTSSSGPGIALKTEAMGLAVAAELPLII
                     VNSQRAGPSTGMPTKTEQSDLYQAVYGRNGDTPLPVVASRSPADCFDTAIEAVRIALR
                     HMTPVILLTDGYIANASELWPLPDFDHYEPIETHKPDPSTDPRHSVFSRDPKTEARLW
                     ATPGDKRFVYRVGGIEKDIHTGNISYEAQNHQEMTNMREAKVASVASFIPDQQVDSGT
                     DAGLAVVAWGSTYGTIYQAVRECVAEGLAVGHIHLRHLNPLPPNLPTLLAQFDRILVP
                     ELNNGQLATLLRDKLVLPVEQHNQVNGQPFAVADVKQAIARYAPKRIKEAG"
     gene            29819..30193
                     /locus_tag="AC16QF_09570"
     CDS             29819..30193
                     /locus_tag="AC16QF_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448846.1"
                     /translation="MKVVVKTDEYTIYQRRDERYAVKDATKAWVNGDAKAAILVQHEL
                     ITAALPAAPLEEPEADAGEPAAEAAAAEEVTGAEESAAEESEPAEAAAPAEEAAAAEV
                     AAEDEAGEPASAEEGEEEPKPE"
     gene            30200..>31352
                     /locus_tag="AC16QF_09575"
     CDS             30200..>31352
                     /locus_tag="AC16QF_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIR6448847.1"
                     /translation="MVKAVAEFEVVSDYEPAGDQPTAIAGLVEGLVDGLSHQTLLGVT
                     GSGKTYTVANVIEAVQRPTLVLAPNKTLAAQLYGEFRDFFPNNAVEYFVSYYDYYQPE
                     AYVPASDTYIEKDSSINAHIEQMRLSATKALLERRDTIIVASVSAIYGLGDPQSYLRM
                     VLHLDRGDRVDQRYVLQRLAELQYTRNDMAFQRGSYRVRGDVVDIFPAESEKEAVRVE
                     LFDDEIENISVFDPLTGEIVNRVPRYTVFPKTHYVTPRERILAVLEDIKEELQDRLAY
                     LQKHHKLVEAQRLDQRTRFDLEMIRELGYCSGIENYSRYLSGREPGEPPPTLFDYLPK
                     DALLVIDESHVTVPQIGGMYKGDRSRKETLVEYGFRLPSALDNRPLRFDE"
BASE COUNT         5408 a         9800 c        10508 g         5636 t
ORIGIN      
        1 gatgcgcggg gtggagaagc agaactctct gatgaaaact tccgccatgc tcggggcctt
       61 ccgcgagaat cccagcacgc gcacggagtt tctgaacctc ctcaaggact gagggcgccg
      121 ccgccagcct cgccggtcag ccgccgaggg aagcgaccag gtccgcctcg tccggaaact
      181 ggccggtttc gtgcttggag taaacggtgc ggccgtccac gaccacatcg aatatcccgc
      241 tgcccccgcc gacgagctcg gcctcgacac cgaaggcttc tcgtatcctg gcagccagac
      301 tgaccgcctt gggttggtag tttcactgca cgcagtaggt gatgcttacg ttcaaggcgc
      361 aggccctttg caatccgatg gtcaggtcat tttaattggg ctgcggggaa tgccaaacga
      421 acatttaagg ggacccatat gaacttcgag atcccggaag acatcgccga gctgctggac
      481 cgcctggacg acttcatcga gcaggtgatc aagccgctgg agcagcagga cgacaacatc
      541 cgcttcttcg accatcgccg cgaagacgcc cgaacggact gggatcgggg cgggctgcca
      601 aacgaggaat gggagcagct gcttttcaaa gtgaagcgtc ttgcagacga ggccggcttc
      661 taccgctacc cctttccgaa ggagtacggt ggtatgaacg ggaacaacct gggcatggcc
      721 atcatccggg agcatctcgc cgtcaaaggc ctgggtctgc acaacgatct gcagaacgag
      781 cattccatcg tcggcaacca ggtcggcctg ctgctcatgc tcgaatacgg caccgatgag
      841 cagaaggccg agtgggtcga cgatctcgcg aacggcgtcc gcggcttcgc cttcggtatc
      901 accgagccga accacggcag cgatgccacc catatggaga cccacgccga gcgtgacggc
      961 gacgagtggg tgatcaacgg ggagaagacc tggaacacgg gcattcacaa ggccaagtac
     1021 gacatggtga tggcgcggac gtcgggcaag cccggggacg gggacggcat caccgcgttc
     1081 ctgacgccca tggaagccga tggcgtcaag atcgaggaga tgctgtggac gttcaacatg
     1141 cccacggatc acggccgggt atcgttcacc aacgtgcgcg tgccccacgt ttcgattttc
     1201 gggggcgaag ggcgcgggct gcaggtggtt cagcacttct tcaacgagaa ccgcatccgt
     1261 caggccgcct ccagcctcgg ggccgcccag ttctgcatca acgagtctgt caggtatgcg
     1321 caggaacgaa agccgttcgg caaggcgctt gcgaccaacc agggtatcca gtttcccctc
     1381 gtcgagctgc agacccagtg cgagatgctc cgggccctca tccacaaaac cgcctgggaa
     1441 atggatacgt acggtgcgtt ctccgtttcg gacaaggtgt ccatgtgtaa ctactggtcg
     1501 aaccgtctgt gctgcgaatc ggccgatcgc gccatgcagg ttcacggcgg tctggggtac
     1561 tcgcggcaca agcctttcga gcacatctat cggcaccatc ggcgctaccg gatcaccgag
     1621 ggcgcggagg aaatccagat gcgtcgggta gccggataca tgttcggatt catgtcgcag
     1681 cgcgcgccca agggtgtcag ggaaagctga aggtggacca cgccgagttc gaagcgcggc
     1741 ttggcaaggt gctggctctt cggatcgggc gcgtccgctc gctgcgctct gccgagcgcc
     1801 tgtccggggg agccagccag gagacgtatc ggcttaccgt cgatacggct gacggcgagg
     1861 agctcatggc catgcgccgg gctcccgggg gcggcttgga cgtggaggtt gcgccaccga
     1921 cgctggccac cgaagccctg ctgatgcgca gtgcccgcgc cgccggggtc ccggagcccg
     1981 aggtctatta cgttctggag ccggaggacg gcctgggcga aggtttcgtg atggagtggc
     2041 tggagggcga agcgcttggc gcgcgcatcg tccgggcgcg cgagctcgac ggcgttcggc
     2101 cgcagctcgc ttacgagtgc gggcgagtga tggcgaagat tcacgccatc gacctggacc
     2161 gtgccgggct cagggagcgg ctggaaactc tgactccgga ggagctgctg catcgcagct
     2221 gggatcgcta caaggacttt cccacgcctc agccgatgat cgacttcacg gcgcgctggc
     2281 tgctcgacaa catgccggcg acgttcgaca tggcgctcgt ccacaacgat tttcgcaacg
     2341 gcaatttcat ggtggacgcg aacgggatcg tcgccgtgct cgactgggag gtcgcccaca
     2401 tcggcgatcc catgcgcgac ctcggctgga tctgcaccaa ctcctggcgt ttcggacgca
     2461 gcgaccggcc ggtgggtgga ttcggcgagt acgccgatct gttccgcggc tacgaggacg
     2521 agtccggcgt ggcagttgac cccgagcgcg tgaagttctg ggaggtgatg gggtcgttct
     2581 ggtgggcggt cggctgtctg ggcatggccg agcactaccg taccggcccc gacaagaccg
     2641 tggagcggcc ggccatcggc cggcgctcgt cggagtgcca ggtggattgc gtcaacctga
     2701 tcattcctgg tgacgtggag ctggtggcgg gcacggatct tgaggattgc acggacatgc
     2761 cccgggtcga cgagctgctg gtgagcgtgc gcgattacct gcgcgccgac gtcatggcgg
     2821 ctacgagcgg gcggacgaac tttctggcac gggtggctgg caactccctg gacatcgtgc
     2881 tgcgagagct ggcgacggga gccgaggcga ggttccgtga gcaggtccgg ctggaagcgc
     2941 tgctggggcg ttcgggggat ctggacgccc tgcgctggga gctcgtccac cggctgcgat
     3001 ccggcgagat ggcgctcgac gacgttgccc tcacggatca cctccgcacc accgtggtga
     3061 accagatcgc catcgatcag cccaggtact ctggtttcaa aacggcgctg gcaagagccg
     3121 gaatcgaggt gcccgaatga acattcgaac cgacgccatt gtgcggctgc gcgatcacct
     3181 gctctccagc gatctcgggg ctgccggcca agttcccgat gagcagcccg gcgatgcggt
     3241 ccgcctgcgg gtagagcctt ttgccgagac catgtttctg gtgatgctgg cggatgccga
     3301 acaggccgtt tcggaacacg acgccctggt ggttgcggtc gacatgctca ccgatggtct
     3361 gatgggccgc gaagccgtgg acgcgctgct ggccgaattc gctgagcgcg ttcgtcggga
     3421 aggaagagaa gattgcgtgg cgcggatcgg agctcggttg agcgcggacc gggacgatcg
     3481 ggaaatggcg tttacgctcg ccgccgtcat cgcgctggca gatgatcgtg tcgcggtgga
     3541 agagaacgag gtgctcgatc tgatgcggac ctactacggg atctcggacc gacgcatgga
     3601 agccatactc gggaccctgg tttagcccag cccctggggg acctccaact cgtcgatctc
     3661 gatgccctgt tcgtgaatgt gcagcgagac gtggtggcgc atgacgtcgt cgctgccgga
     3721 gtagatccgc ggaacccggg cggcgttgac gtagaggatc ccgtcgtgct cgcggcgcca
     3781 ggggcgctcc tggccgcact tggtcttgag atgcatatgg cccgcgacca ccgccagcac
     3841 tcgtcgcccg gtcgcgacgg cgtgagcaat ggcttctcgg agatccggat cgccccaatc
     3901 tcccccatcg ggtttgaagt cgcagcccca catgtctgcc ggctccccgc ccaggcccgt
     3961 gggtccgttg tgggagagga acacgaggtc ggcggtttcg gccgcgtcga ccagttcctt
     4021 caggcgcttg gcagacgctt caaggctgct gataccgtac gtggcagaca tgtggtcggc
     4081 gaaggtcagg gtcgggccgc ccatgctgtg gggccgggcc gcgatcagcg tcacgtccgc
     4141 agcgggggtg cgatagcgat ggctgctgta cccgcaaagg cgaataggct tgatcgcgtc
     4201 gccggcgccc tggcggatcc cggtgagcct tttcaggcct gagcggtggg aaagctcggc
     4261 agccagctgg tgaatgtcgc cggtatcgtt gtttccgggc atgaccaggg tttccacgtc
     4321 gagtcgcgag atcgttcgcg ccacccgcag gctgctctcg acgctgccgc caccgaggtc
     4381 gcccgtgaag aacagcaggt cgtagcgcgt cgcgttgaac tgcgccacgt cgatatcgtc
     4441 ccagtgggtg tgcaggtcgc cgaccacgcc caaacgcagc cccgtcatgt tctttgatgc
     4501 tccgtgcggg ggagggcttc caatcttagc ggcttgatgc cctgcgcgaa aatccttggg
     4561 ggaaggtttc tcagccccgg cgtggcgagg atcagccggg cgggaagcgg cagatggaac
     4621 tcctcgtctc tcagtgcgcg cgacaccacg gtgtcctgta tgcgcgcctg caagccctga
     4681 atgatccggg tgggaacctc ccggcgccgc tgaacctcgg ccagggccgc ttcgccggga
     4741 gcgacggcgt ccagcagggg tcgagtcaga acgttcgctg cctcgacggc gtcgccaatg
     4801 gccgcattga tgcccacacc ccccaccggg gacatgacgt gcgcggcgtc cccgatgcag
     4861 aggagcccgg gccggtgcca cgtcggcagc cggtcagagc tcaccgtcaa caggtgcacc
     4921 ctttcccagc cgtgaatgac gtcggtgcgt ccggccagcc agggcgcggt tgccgcgatg
     4981 gagtcccgga acgcctggat tccggattcc ctgagcgtct tgaagtctcc cttgggaaac
     5041 acgtagccga tctgccactc gtccgaacgg ggcagcgcga tgagcatgcg gccttgcccg
     5101 acgaagaaac cgcccgcctc gtggccgtcg gatgcttccc ggggcagccg gaaccagcag
     5161 acgtccatgg gcgggctcag gctttcggcc tccagcccgg ccagcttgcg cagccgcgag
     5221 aagcgcccgt cggcggcgat gaccaaatcg gcgcgcagct cgtgctcctc gccgtcgcgc
     5281 cgatagcgga cgcccgagac gcgtccgtcc acctcaacga gctgttgaac ctgggcgccg
     5341 agaacgcacc gaaatcccgg gtagcttttc gcctgctcgt gaaggtagtt gaggaagacg
     5401 acttgaggca tgaccatcac gtagggaaag cgcgtcttca gccgcgaaaa gtccaccagc
     5461 tcgagcgtcc gcgcggcggt gtggagagtg acctggcgca tctttgcgtg gggcagcgcg
     5521 agcaggcggt cggccaggcc gatctgatcg agcacctcca gcgtggaggc atgcacggta
     5581 tctccgcgga agtcgcggtc caggtcgcga tggagctcga ggacggtgac cgtcactcct
     5641 cgccgggcca aaagaagtgc caggaacatt cctgcggggc cggcaccgac gatgcagcag
     5701 cgggtttcgt tgctcatgcc gacaggctat gtcgttgccg acgggcgcgc aaatccatac
     5761 gggacaatgc ggatgggccg gctcggccag ccatgtcatg ctaaatagat gtactgcgct
     5821 gcatcgaaag gactgcttgc ctgggcgttc gggagtttcc tcctgatggg ggcgacgtcc
     5881 gcgctgcacg ccgcagagcc ggaagagctg ctcaagcgcc ggctgatggc agagatccgt
     5941 ggtgatgcga agcgcacggc ggactatacc ggccgaagtg ccctcgacga acgtgtgatg
     6001 gcggcgctcg aggccgtgcc ccgccacgag tttgtcgagg cctggagccc cggcgcggcc
     6061 tgggagaacg tccccttacc cattggtcac gggcagacca tttctcagcc gttcatcgtc
     6121 gcgctcatga ccgacttcgt cgagccccac ccgacgcacc gggtgctgga gatcggcacg
     6181 gggtcgggct atcaggcggc cgtgctcgcg gggctcgtgg gcgaggttta cacggtggag
     6241 atcattcccg cgctcgcgca aacggccacc gagcggctgc accggctcgg ctacgacaac
     6301 gtcttcgtgc gcgtcggtga cgggttttac ggttggcccg agaaaggtcc gttcgacggt
     6361 atcgtcgtaa cggccgttgc gccagagatc cctgcgccgc tgctggagca gctgcggccc
     6421 ggcggtcgga tgatccttcc cgtgggcgag gtgcatggcg gtcagaacct ggtgctcgtc
     6481 agcaaggacg ccgacggagc ggtggcgacc agggacgtgc tgccggtaat gttcgttccg
     6541 ctgaccggcg accactgacg ccaccgacct cagatccggg aacctttctt gaactccgga
     6601 tcccgcttct ccttgagtga gctgaccccc tccctcgcct cgccgctcgt gaacccgagc
     6661 atctcgagtg ccgtggacgc atcgaaggtc gggccggcca gacgcagcca gttgttcagc
     6721 gcgtacttgg tccagcgaat ggcgttggga gcgccgttgg cgagctgcac ggcgaccgac
     6781 agggcccggc tctcgagctc ttcgtccggc acgcacatgg agaccaggcc gatgcgctcg
     6841 gcctcggcgc cggacaaccg gtcgcaggtc agaaggtagt actttgcctt ggccatcccg
     6901 caaagaagcg gccagatgat ggcggcgtgg tctccggccg cgacgccaag gcgcgtgtga
     6961 ccgtctgtca ggctgacgga ctccgccacg atggagatgt ctgcgagaag cccggcgacg
     7021 agccccgcgc ccaccgcggg cccgttgatc gccgatatga tcggcttgcc gcagttgatg
     7081 atgttgtaga ccagatcgcg cgcttctttc caggtctgcg cgcgggtgtc gaagtcggca
     7141 atgatctctt cgatcatctc gaaatcgccg cctgccgaga aagccttgcc cttgccggtg
     7201 aggatcacgc agtcgatgtc ggggtcggcg tccacttcgc gccagacgta ggtgatctcg
     7261 ttgtgaccga cgacgtccag ggcgttgtag cgctccgggc ggttgaagct gatgcgcagt
     7321 atccgtgggt gggggtagtc gaactccagt cgctggtatc cctggtagcg ttcggtcatc
     7381 tggttcccct cgtcctgctg tcaattggct caggggaacg gtacgctttt tgggagatct
     7441 ggcaagtcgc cacgcgtttg cgcacggcgg cacaatcgac cgggcgttga gctgccatca
     7501 tcggctgtgc caggtcaatg ctcaaaggag tcagccatgc gcctgctttt catctgcctc
     7561 gtcgccctgg ccccggtggc tgccgcccat acgcccgttc ccgtgcatgg atgtgcattt
     7621 cccgagcggc cgccggacga cgtcgacgag atgacctggg accgcttcct ggaaggcgtc
     7681 gagcggtatc gcgcctgcat cagcgacttc gtgcgcagca actacgcggc gtccgatgcg
     7741 catcgggacg ccgcgaacgc ggccaccctg gactggaacg cgttcgtccg cgattccctc
     7801 aacgttcctg aggatttccc ctgggagccc gaggactccg acgaccaggc ggagccggac
     7861 aatccttagc ggctgccgcc gccgagttgg tctgcggagc gctaaaggcg gataatgccc
     7921 cctgcccagc gaccaccagc ggcggacaga tgcagcaact caccggacta gacgcctctt
     7981 ttctctacct cgaaactccg aagacgccga tgcacatcgg cggcgtcatg atctacgacc
     8041 agcgcacggt caagggtggc aagctgcgct tcaaggacat cctcgatcac ttcgagagcc
     8101 gtctgcatct ggccaggacg tttcgtcaga agctcgtgca cgtgcctttc aacctggatc
     8161 atccctactg gatcgaggat cgcgatttcg atctggaatt tcacgtgcgt cacatccgtc
     8221 tcccggagcc cggtgactgg cgtcagctct gcattcaggt ggcgcggctg cattccaggc
     8281 ctctggacct caccaagccc ctgtgggagt tcaccatcat cgaggggctg gatgacattg
     8341 aagggcttcc ccggggaagt ttcgcggtgg tgaccaagat ccaccacgcc tgcatcgatg
     8401 gcgtgtccgg cgtggacatg gtggaggcca tccacgacgt cgagccctac gtcgaccggt
     8461 cgcccggacc ggtcacgccg tggaagggcg agcaggagcc cagtgccatc gagctcatgg
     8521 tccgtgccca ggtccacaac atgacgcagc cctaccgcct tgcggaggtg ctggcccgta
     8581 cggtccccgc cctgggcagg ctcggccagc gggcgacggg gggcgacgcg gaacgggctt
     8641 ccctcgcgcc gccgcgaacc aggttcaacc acgtggtatc ggctcatcgt gtgatcgagg
     8701 ggcggcgatt cgacctggcg gagataaggg ccatcaagga caccgtggat ggggcgaccg
     8761 tcaacgatgt ggtgctggcc gtctgcggcg gcgctctgcg gcgctacctg ctggcaaagc
     8821 acgagctgcc cggcgtgccc atgatcgcca tggcccccat ttctgttcgc agctccgacg
     8881 agaaaggcgc catgggcaac cggatctccg ccatgacgat ctcgctgggc actcacatag
     8941 aggaccccat cgggcggctt agcgccattc acgaagcggc ggtggcctcc aaggggatga
     9001 gcaacgcggt gggcgcgaag ctgttgaccg actattcgca gttcatcccg tcgacgacgg
     9061 cagccatggc ggcccgcctg tacgttcagc tggggttgag cgagcgggtc aacattccgt
     9121 tcaactgcgt cgttacaaac gtgccggggc ctcagtttcc cctgtacagc gcgggagcga
     9181 agctgctggc gcagtatggg ctgggcccga tattcgacgg catggggctg atatttcccg
     9241 tgttcagcta caacgggaag ctctttctgt ccgtcacctc ctgccgggag atgatgcccg
     9301 atccggagtt tttctcggaa tgcatgcagg cggctttcga cgagctcaag gcggctgcgc
     9361 tggtcattcc ggaagccgct gacgcctcgc tggccggtgg aacgccctga tgctcgggag
     9421 ccgttcgcgg ctacgcgtcg tccctgcgcg tcggccccag tggcgagttg aaggagaaca
     9481 gccgtttctg gagccgggac agcatgcccg gggtgacctc ctcgtcgaag tcgtcgatgt
     9541 cgaatcgcac gcgcagaatg ccggcggcgt gctcgatgcc ggccagacgt atctcgccgg
     9601 acaggggcgg gaccggcaga tagccgcgga aaagggatga cacgtagccg ggcacaccga
     9661 gcacggtggc tcgtttgggt tccagaacga accacccgtc cacgaccgtg aggcccgtct
     9721 cgaactcggc tagaggaaat ccggccagct ccgtatgcac ggtgatcccg gactccgtga
     9781 gctcgagccg atacggcagc gagaaccgcg actgccagcg gtcgagctct gcctggccca
     9841 cgacgaactc cacgctgggc gaccgcacgt agagccgagc cggaaacccc gggacgaatc
     9901 gcacttcacc ggcacgccag atcacgctgt ccaggcgcag ccaggaagtc gtcatgcccg
     9961 cgaattcgag tcgtgcgtcg atgaaggtgt cggggaaagg gagcccgcgg gtctcgcgcc
    10021 agtagatctt cagcggaatc ttaaggtagg aagcgaccag ctggtcgatt acgtctgaac
    10081 ggctcgtcga atcggtctgg cccatgagcg gagtttaccc aggctttgcc tgggaacaaa
    10141 tacgggttaa gtttccgcca tgtccggcca gaccaacagc gttggtgggg aaaaggtgcc
    10201 gctcgtcatc ggcgtgacgg ggcatcggga tcttctcgct gaggagctgc cggtgatccg
    10261 gcggcgggtc caggatctgt ttgccgagct gcagcggcag ttccccgatc tgccgatcct
    10321 ggtcatgacg cctctagccg agggggcgga ccggctcgtt gccgaggtgg ccagggacat
    10381 gggcatgccg gtcgaggttc tcatgcccat gcccagagag ctttaccagg cggatttctc
    10441 gggcgaatcg ctagtcgagt tcgacgagat gctgaggctg ggagaaattg tggagttgcc
    10501 cctcgtgccg ggcaacactg agaagacggt ggcggccggg gggcggcccc gtgacctgca
    10561 atatgctcag ctgggagcat acctcgcggc tcacagtcat gttctgctgg ccatctggga
    10621 tggcaagccg cccgaagcgg tagggggcac ggcgcacgtg gtgcagtttc atcagcacga
    10681 cgtcatcgag cttctggcgg aaggacagcg ccgctcgccc atcgatttcg cggaagacga
    10741 gagcgatctc gtgtttcaca tagcatgctc gcgtctggcc gacggggcgc cgcgagagcc
    10801 gctgcaaccc ggtcaggcgg agtggctcac gcgtgacgac gtgcaaccgc ggacgccgca
    10861 gatgccgccg aggtatcggg tggtattcga gcgcctggtg gagttcaaca gggacctggc
    10921 tgagccgatc ccggcaggct cgctggatcc tctggtcacc ggcgacgatg ccgatcattg
    10981 cgggcccggc gccgaggaca tcgagcgtct gtacatggtg gcggactttc tggctcggca
    11041 ctacaagaaa tatcggcgac ggttgcagac cgccacctac gcgctggcgg tgtgcgccat
    11101 catgagtttc atcgtgtggg ccgatctgcc ggaccaggag atcatgatct acccctacct
    11161 cggtttcatt gggctcgtca tcgttctggt ctatctggag cgacgcaaag gctggcaacg
    11221 ccgatcgctg gaatatcgag tgctcgcgga gggccttcgc gtgcagttct ggtggagcat
    11281 ggcgggggtg gtcatggaga accccagccg cttcagccac gacagttttc tgcgccagca
    11341 ggatctggag ctgggctgga tccgcaacgt gatgagattc gcgggtcgcc gggcggacgc
    11401 caacatcggc aacgaggacg gggccgacac cgacctggtg gcccgggtct gggtcgcgga
    11461 ccagaaggcg tactatcaga gcaagtccca ggagcgcaac agaggcagtc agttgacctc
    11521 ggccctcgtc ttcgccacat tcggagtcgg cgtgacagtg gcgatcatct ttgcggtctt
    11581 tcaggcggag attgccgcgc cctggtccaa catgctggtg gccctgatgg gattgttacc
    11641 ctttctcgct gcggtacggc agagctatgc caacagcacc gcggagcgag aggacttcgt
    11701 tcagtttggc tacatgtacc ggatattttt caatgcggac cgcctcttga attctgcccg
    11761 cacggtcgac ggccgccgcg acatcctccg ggcgctcggg gaggcggcgc tcgaagagca
    11821 tggtcaatgg atgctccgcc agcgtgagcg gccggccgcg ggccgggttc tgcagggcgg
    11881 gtaatggcgc gacgattcac accgcgggaa atgctcgaaa agctgatcgc ctttcccacg
    11941 gtctcgagag actccaacct cgagctgata gagtttgtcg ccaactatct cgacgatcac
    12001 cgcgtgcagg ccagactcgt cgccagcggc gatggacgca aagccaatct gtatgcgacg
    12061 gtcgggcctg agatcgaagg tggcgtcgtg ctgtcagggc atacggacgt ggtgcccgtg
    12121 gacgggcaga actgggactc ggacccgttc cttcttcacg aggtcgacgg caggctggtg
    12181 ggtcgcggta cctgcgacat gaagggtttt cttgccattg ctcttgctct cgtcccggac
    12241 atgggggcgc ttcgctatcc gattcacctg gccttctctt acgacgagga agtgggttgc
    12301 ctgggcgcgc ccgatctcat tcgggagatg acggccaacg tgcctcgccc caaggcggtg
    12361 atcgtggggg agcccaccag catgcaggtg gtgggggccc acaagagcat gttcatcttc
    12421 gataccgtcg ttcgtggcca cgaggcgcat tccagccagc agcacagagg ggtccccgcg
    12481 gtcatggttg ccggtcggct gatcggctgg ctcgccgagc ggcagcgcca gaacgcccgg
    12541 gtagcggacc cggattccga tttcgtgccg ggctacacga ccctgcattg cggcacggtg
    12601 cggggaggga ccgcccacaa catcaccgcc gccgaatgcc gtttcgcgac ggacatccgc
    12661 tcgcttcccg gcgagcccgc cgaatcgtat tttcgggagt tcgagcagtt cgcaaggcag
    12721 acgctcgagc cggaaatgcg cgcggtgcat ccgggcagcg ggatcgactt cgagatccgg
    12781 gctcaggtcc cagcctttca ggcaacgggc gacgacccgg cggtggcact tgccagccag
    12841 ctgaccgggc agaatgggct ggaaagcgtg ccgtacggag ccgaggcggg gcagtttcag
    12901 gaagccgggc actcggtggt catgtgtggc cccggcagca ttgatcaggc ccatcaaccc
    12961 aacgagttcc tcaccctcga gcagctgcag gccggcgcga cgtttgcgcg acgcttgatc
    13021 gagcagcttt cttgattaga aattcggccc cgaggcacga taaccgtgag acgtctgtca
    13081 atcgatttgt gaactgattt gtgtagcatt tgccagcgca taggtgaaga ttgccgacgt
    13141 tgcgaggctg catcgtcagt ggtcatacgg acatgaattg ggtttcccgg cgctcctgct
    13201 ccgatcggag gccggtagag atggatggcg actcgatatg acggcgcggc ggccctcgaa
    13261 aggcttttcg ctgatagaac tcatggtggt ggtgggtatt ctcgccgttt tggcagcctt
    13321 cgccatgccc gcttatcttg actacttgga gagcgccaac accagcaagg ttctttctca
    13381 ttacgaggat ggcacgcgct acctcaggac ggaaattctc aggctgaaca cgtcggtcgt
    13441 catgggaaaa gaaaccctcg aatccattga cgcgaacttc acgtctgcat tctgggtcga
    13501 caagctgaac caggaaggag ggcgcgctcc cggagggggc cctgcttacg gagcctccgt
    13561 cgatcacggc atgggccgtg tgggtgtggt ggtcgctggt agtttgttgg acgctccgcc
    13621 atcctatcga ctcgaggtga ctcgtcccca gtacggcggg ttcgcacagt actcggtgat
    13681 gaccgcgatc atttcctgga gtggtggcgg cgggggaaca tcgtttggct cgcaggctga
    13741 tcccacccct ggccagcctg gcggcttgga tggttccggc ccgggccagg gcgcaggaag
    13801 cgcagacaat cctggccaag gcaaagatca ggggcaagac aaaggcaaag gtcaaggcca
    13861 gggtcagggc caaaacgttt agggtggctt tacagccgct ggctgagcgc cgatctcgcc
    13921 ggagcgtcgt ggcggcaggc tgtcggaaat ccctgggcgg cggcaacggc ctccacttcc
    13981 ctccacacgc gcatcaggtt gccgccgaga atcagggaga tgctgtggtc gtcatacccc
    14041 cggttggcca ggccggcaat caggttcggg tacgagctga cgtccttcag acccaccggc
    14101 agggtgtcgc ccacgccatc gtagtcggaa ccgaggccga cgtgctcgat gccggccagc
    14161 gcaacggcgc ggtcgacgtg attcaggacg tcttcgagct ccgcgtaggg atacgggtgc
    14221 tcggcgcggt agcttgcggc gaacgcccgc acggccggat cgtcgtagcg cggctcctcg
    14281 gtttcggcga atgctttcac ggccgcggtc atggcgtccg agtacgcccg ggcttcggcg
    14341 gtgacgaagc tgctgccgaa attgatctga acgacgccgc cgttgcgtcc cagcgcggcc
    14401 accatctcgt cactcatgtt gcggtgaaag cccggcacga aatggcgcag agaggagtgc
    14461 gttgccacca ccggcgcgct cgagagctcc agcacctgcc agaaagcgcg gtcactgagg
    14521 tgggacacat cgatcatcat gccgcgctgg ttcatcgccg ttaccaggtc tctgccgaag
    14581 ggcgacaggc cctcccaggg ctcgttctcg tcgtacgagg aatccgagat gtgattcgac
    14641 ttcgagtgcg ccagcgtgat gtagcggacg ccgcggccca tgaagtgatc gagcttgccc
    14701 agatcgccgg caatggggcc gccgttctcc atgcccattg gcagagcgat gcgtcccgac
    14761 gcgcgaatgg cctccacgtc tcgggtacag gttgccacgg cgaacttgtc cggcgcgtcg
    14821 cgggcgatgc cctccaccag gtcgatgaga cgatcggcca tctcggttgc gcgtccttcg
    14881 gcatcgacgg cggcggggac atagatggac atgaacgccg cgtccagtcc tccggcgagg
    14941 gccctcgggt aatcgaactc gccgctcgtg ggctcggcga cgtcttcgtg gcgccggtaa
    15001 agccggtagg gcaggtcgat gtgggtatcg acgatgagct cgctgcggga gagagctcgc
    15061 gcgtgctcca ggtccgggct cactccgggc tctccgcagc cggcggcgac ggtggacaat
    15121 agaatcagaa ttctcgagcg tcgcatgtga gccatctccg cggagggatt atgttcacac
    15181 gcccgaaccg cgcagcacaa gctgagttaa aatgcgctct gcaaccgggc aggaatcaag
    15241 atgaccatca ttcgtgagga tgacctcatt gaaagcgtcg ccgacgcgct gcagttcatc
    15301 tcctactacc atccgctgga cttcatccgc gccatgcgca gcgcatatga gaaagaagag
    15361 tctccggcgg ccaaggcggc gctgacccag gttctggtga actcgcgcat gtgtgccatc
    15421 ggcaagcggc cgatctgcca ggacacgggc atcgtcaacg tgttcctcga ggtcggcatg
    15481 gaggtctcgt ggtcgggcaa gcgaagcgtc gaggagatga tcaacgaggg agtccgtcgc
    15541 gcctacatgc acccggacaa cgtgctgcgc gcgtcggtgc tgaccgaccc ggacggagcg
    15601 cgcagcaatt ccagggacaa cacgccggcg gtgatccaca cccggatcgt tcccggtgac
    15661 aagctttccg tggaggtggc cgcgaagggt ggtggcagcg aggccaaggc gaagttcgcc
    15721 atgctcaacc cgtctgactc catcgtcgac tgggtgctgg acgtggtgcc gaccatgggc
    15781 gccggctggt gcccgccggg gctgctcggg gtcggcatcg gcgggacgcc ggaaaaggcc
    15841 atgctgctcg ccaaggaagc gctcatggag cccatcgaca ttcacgatct caaggcccgc
    15901 ggtcccgcca accacctaga agagctgcgt ctcgagctct tcgagaaggt caacgcgctt
    15961 ggtatcgggg ctcaggggct cggtgggctg accaccgtac tcgacgtcaa ggtgaaggac
    16021 tacccgaccc atgcggccaa caagccggtg gccgtgatcc ccaactgcac ggcgacgcgt
    16081 cacgtgcatt tcaccctgga cggatccggg cccgctgtct tcgaggcgcc ggatctggac
    16141 gagtggccgg acatcgcttt cgagctgggc gacgacgtgc ggcgcgtcga catggatacg
    16201 ctgacgccgg acgatgtgcg caactggaag gcgggagata cgctgctgct caccggcaag
    16261 atgctgacgg gccgggacgc cgcccacaag cggctggcgg acctcatcga gcgtggcgag
    16321 cctctacccg tggatttcac caatcgcatc atctactacg tgggccccgt ggacccagta
    16381 cgcgacgaga tcgtgggccc cgcggggccg acaacggcaa cgcgcatgga caagttcacc
    16441 ggcatgatgc tggagaagac ggggctgatc ggcatgatcg gcaaggccga gaggggtccg
    16501 gccggggtcg atgccatccg cgagcacggc gcggtttaca tgatcgccgt gggtggcgcc
    16561 gcttacctcg tcgccaaagc gatcacaagg agcaccccgc tggccttcga ggacatgggg
    16621 atggaggcca tctacgagtt cgaggtcaaa gacatgccgg tcacggtggc ggtggattcg
    16681 accggcgcat cggtgcatcg cgaaggaccc aggctgtggt cgcagaaaat cgccgatcgc
    16741 ataggcgctg cccagttgtg acgacccgac ttcactatcc ggcggaggct gtgtgatggc
    16801 aacaagaagg agaacgctgg cgatgggcct ggcgctgctc gccgggggat acctcggccc
    16861 tctcgcccgt cacgcccggg cgcagtcgct ggaacaggcg atggaggaat cggacctgat
    16921 ctacctgact cctctcaaga gcgatggctc ggagagccgc tgccgggcgg aggtctggtt
    16981 cgcgcgtcat gaagccgatc tcttcgtcgt cacgtcgagc gacgcctggc gggcgagagc
    17041 cgtcgagcga gggctcacgc gcacccgggt ttgggttggc gacgtgggcg tctggtccgg
    17101 cagcggcggc agctaccgaa cgttgccgca gatcgaagcg gatgcctcga tcgtggcgga
    17161 ggccgacgtg cacgacgcgg tgctcgacgt catgggtgac aagtaccgga tgtcctggct
    17221 ggtctggggt ccccgattca ggaacggtct cgcggacggg tcgcgggtca tgctccggta
    17281 ccgtcctgct gccgcctgac cgaccacgaa catcgaccgc ttctggccgt gaggtcagac
    17341 gtagtgcagc cagtggcgga aggtgggctc ctcgccggac gagatctttc tgagggcggc
    17401 acgtagtctc ttggtcaccg ggccgggcgc cccgtcgccg aacaccacgt cgtcaacgcg
    17461 ctcgatgggc cacacgcccg tggaagtatt ggtgaggaac gcctcgttgg ccgcgtagag
    17521 ctgcgtcagt tccagctcct gctcctcgca gggcaggttc aggaatccag ctatctcgat
    17581 gatcgatgcg cgggtgatgc cgagcagcac atcgttttcc gggggcgtcg ccagcccgcc
    17641 atcccgcacc acgaaaatgt tggacgttgg cgcttccgcc acggtcccgc gctccgtcag
    17701 gagcaggacg tcgtcaaaac cttcggccct ggctttgcgc ttggctatgg tcggtgaggt
    17761 gtagtttgct gcgaccttgg cttggggcgg caggatgtcg tgacgccgat tgctgatctc
    17821 gcgctctact ttcagggcca gggtctcccg tgtcggatac ggggtctggt gcttggccat
    17881 gatgtctctc gcgctgtcgt acgccgctat gaatacggcc acagcgggat cctgaggtat
    17941 gagctcgacc tcgatggagg cgatcaacgc gctgatcttc acggatttgc agccggggtt
    18001 tcgacgcacc gtctccgcgc aggcttccat cagcgcctcg gccggataca ggatgggcaa
    18061 ttcctccagg cggcaacttt gcagcagacg ctcgatgtgg gcttccaggc ggaagatcgc
    18121 gggcccgtcc gcggtttcat gaacgctcat gaagtcgaag gccagggtgc cgcgctgcag
    18181 gctctgggcg agcacgtgca cggtagcgtc tgcccagggc acgaactcgc cgttgcgcca
    18241 gatcacccga tcggatgctg aggaattcat gtggtcgtcg tttccttaag cttctctacc
    18301 cgtgttcgcc ggcaccccaa acggcctgct gggggaaagc caccaggctg tcgtggtagt
    18361 cgagacccgg ctgccggtcg cgcacgagca gcagcggtcc gtccaggtcc acgaagcgcg
    18421 cagtctgcgc caaaagcagg gccggtgcca tggagagcga cgtcgccacc atgcagccca
    18481 ccatcacgtc catgccgagc gtttccgcct tcttctgcag gatcagagct tcggtgagcc
    18541 cgccggtctt gtcgagcttg atgttcgcga cctggtagcg gccagcgagc cggtcgagat
    18601 cctcaccggc gcgcagactt tcgtcggctg ccagcgggac ggggcagcga aaagcgtcca
    18661 gagcctcatc ctcggatact ggcaaaggct gctcgatcat ctccacgccc agatctgccg
    18721 ccaccggagc gacctgctcg agctctccca gcgtccagcc ttcgttggca tcgacgatca
    18781 ggcgggcgtc cggggcgccg ctgcgcaccg cgcgcaggcg ctcgatgtcc cgcccggcac
    18841 ccgcgccgag cttgattttc aacagcgaac ggcgagagtg ccgttcagcc gcttcacgca
    18901 tctgcccggg ctcgccgagc acgatggtca gcgccgtgac cacgggccgc ggctctgcaa
    18961 gccctgcgag cctccagacc ggctgtccgg atttccgcgc ctccagatcc cacagcgcgc
    19021 agtccagcgc gttcctcgct gggccggcag gcatcagctc cgtcaaccgt tgacgatcca
    19081 ggatggcgtg gccggctgtg gcgacccgga tctcttccag ggttccctcc ggagactgtc
    19141 cgtagcgggg atagggcaga cattcgccca tgccggtcag gtcgccctca cgcacggtca
    19201 ccacgaccac ctcggccacc gtcttgctgc ctcgggcgat ggtaaaagtg ccggcgatgg
    19261 gccaactctc gatgtgtgcg aaaacgtcca tggggagctg gcggctttca gtccggaaag
    19321 tttttgacaa gctcatccac gagcaagccc gcgccgaagc ggatgggatc cgtgacgggc
    19381 agcccgagct gcgcggcgta gttggtcagc agctcacgag cctcgccctc gtcgagtgca
    19441 gccgtgttca ccgcgacgcc gatgcagacg acgtcggggt tggtggttcg accatgcacg
    19501 agcgtctgct cgatcacctc gccgatgcgc ggcatgagcg tgctgacgtt tcgcatggtc
    19561 cggcgggtcg gctcgtggca gaccacgacg gcgtccggct gggtcccgtg cagcagcccg
    19621 agggaaacgc cggcgaaaga agggtgaaag atcgagccct gaccctcgac cacgtcccag
    19681 tggccgcgtt ccgcatccgg ggtcaggcac tccgcagcgc ccgagatgaa gtccgccgtg
    19741 acggcgtcga tcgcgacgcc gctgccggcg atgagaatgc ccgtctggcc cgtggccctg
    19801 aactcggcgt ccatgcccct gctgcgcatc tcgcgctcca gggcgagcgc cgcgtacttc
    19861 ttgcccaccg agcagtcggt gccgacagtg agcaaccgtc ggccgctacg ttttctgccc
    19921 gaccccgtag ggaaacggac ggtgctgtga cgcacatcga ccagacgacg atcgtggcgc
    19981 gccgcagcat cccggatctc cggaaagctg tccaaccggt cgtgcattcc gctcgcgatg
    20041 tcgagaccgt ttgccagcgc ggcgtgcagg atgggcagcc atttctcggg cagcgaaccg
    20101 cccgggctga ccgttccgat cacgagggtg cgggcgccgg ctgccgccgc ttcggccacg
    20161 gacaaatcgg gtaggccgag atccgcctgg cactcgggca atctcagctg cccgatgcag
    20221 ttctgcggcc gccagtcgac gatgccctgg cccgttttcg cggccagctg gtcgtgggcg
    20281 ctgcccagga agatcaggta aggcgcttcg atcctcgtgc cggctgacgc catgtcgttc
    20341 ggactgcgca atctgggaac ctccgccagg tcgccgtcgt ctctgatcaa aggcggtaca
    20401 cgcgggtcgc ggtgccgctg aacaacgcat cctgctccgc ttcgctgaaa tcggcggcga
    20461 tgcgcttcat gccgttccac agcaccgcgt atgacagcga catgcgatcc accggaaaat
    20521 tgctctcgaa catgcaccgg tcggcgccga acacgtcgat catgtgccgg tagtaggggc
    20581 gctgggcctc ggcgaattcc tcggaggtcg gcggccgggc agcggcgtgc caaccgaaac
    20641 cgttgtccgg catggccagc ccgcccagct tggcgaccac gttggggcag tcggctagcc
    20701 gggacatgtc atcctgccac tggcgaaaga tctcttcgcg ccggtccgcg taaggaccga
    20761 cgcccagcgg ggtgccgaaa tggtcgagca ccatgatggt gtccggtacg gcccgtgcca
    20821 gcgccagaaa ctgggggttc tgatagtgat agtgccaggt gtcgtaagtc aggcggagct
    20881 cgccgagccg tctgacgccg cggcggaagg catcgtttgc atacagctgc gcgggggccc
    20941 ggccggcgat tcggaggatc tcgggctccc gggcgtgggc ccctgagtgg cggatgcctt
    21001 tgaacaggtc tcccgcgacc tcgacgtggg cgtccagcac ctcgtccagg gcgtcgccaa
    21061 gccgcagatc ggctcgggcc acgatgccgg caatcaccgc atttccgtcg ctttgagcag
    21121 aggaggcgcg ggcggcctgc gtgacgtatt ccgtctcgcc aacgcaacgc aggtgctcgg
    21181 ggcccgattc ccggtaactt gctccgcatt ccagaaacac ggttttcgcg atgcggtggc
    21241 ctgaccccgt gtcgcgccac aggtcttcca gcgtgtagcg tccgaacgcg cttggctggt
    21301 cccagaggtg gtgatgggga tcgacgatca cgcgatcggg atcgatgatc ggttcctcaa
    21361 cgaggtccag ccaggcctgg ctgccaggtt cggcggtatc caaggcgtcg tctccgagcg
    21421 gccattcagg agtcgtcatc ttagtacagg acggctcggt catgctcgcg tctggatggg
    21481 gcggtggggt ttggcttacc ccggagcgct gcccgggtcg tcagggcgct cgcgtcagcc
    21541 gcccggctgc agtttgcccg tcgagcggat atttgagcga tgctcttcaa cgccctgggc
    21601 gacggggtcc cagccggaga cgtcctcggc caggtggtaa agcgcgggca ctagcagcaa
    21661 cgtgatggcg gtcgcgaaca gcacgccgaa agccagcgag atggccatgg ggatgaagaa
    21721 tgcggttgcc ggggtgtcgc tgaacatcaa cgggatgagg ccgacgaacg tggtcacgga
    21781 ggtcagcagg atgggcctga atcggaccac gccggcgctg cgcagggctt ccgcgatcgg
    21841 catgccctcc cgacgacggc gattcgtgta gtccaccagc acgaggctgg cgttcacgac
    21901 caccccggac agagccacca tgcccagcgc cgagaagaac atcagctgat agcccatgag
    21961 ccagtgaccg acgatggcac caacggctcc gaagggaatg acgctcatga tgaccagcgg
    22021 ctgcaggtag gatcggaggg gaattgccag cagcgcgtag atgacgatca gagcgagcat
    22081 ggcggcctgg gccagtcccg tgaacgcttt ggtcctctct tcctgctctc cggagacgcc
    22141 gaccttcaaa gaggggtact tggcgcgaag cttcggcagc accttggatg agatggacga
    22201 accgatctcc tcgggcgaaa cgacgcttcg gtccacctcg gcggtgacgt tgatcaccct
    22261 gcgcccgttc acgcggcgga tcgtagagta accacgaccc agctcgaact tggccacgct
    22321 gtagaagggt acctcggtgc cctggggcgt gcggatgtac atgtcttcca ggttgcctat
    22381 ggacgttctc tcggcctcgg gaaatcgcac catgacgcga acgtcgtcct ggccgcgctg
    22441 aatgcgctgg gcttcgtagc cgtagaacgc gctgcgcacc tggctggcca gatcggcaag
    22501 ggtcagtccc agattgcggg cctcgggaag cagggagagc ttgatttcct gcttgccggc
    22561 ccggaacgaa tcgccaatgt cgtagacgcc cacgtagcgg ctgagctcgg cgcgaagatc
    22621 cgtggccgcg gcgcggatca cgtcgggatc gccggcggag agctcgtagt tgatggcctc
    22681 gcccaccgag aaggcatcgg ccgtgaagga gagtttcacc gcgtcgggaa tgttgcccac
    22741 cagcttccgc caccgatggg cgatttcctt ggcgctcagg tagttgcgtt cctcccccgg
    22801 tgccagctcc agcacgacct cggcgaagtg cgaccggccg gcgcttccgc cacgtgatgg
    22861 accgttctgc cggtccgcgg gcatgccgat ggaggtgaac acgttgcgaa tcgttgcggt
    22921 ctggttcagc tcggcgtcga gcgccttgcc gagccgctca ccggcggcct cgatgatgtc
    22981 gacggcctcg ccggtcgcct ccaccgaaat gccttccggc atctccagag tggcgtagat
    23041 gcggtctccc tcgatgcccg ggaaaaaggc aaacaccact cggccgctgg caatcaacgc
    23101 cactgacatg atcagcaggc cgagccccac ggacgccgtg atgtagcgat actcgattgc
    23161 cttgcgcagg aagggctcgt agcggtagtt ggcgatgttc tccagccagc gcgccaggcg
    23221 gccctggatg cggttccagc gccgggagaa agaattggac gcctcgtcgt tattgcggtg
    23281 ggcgaggtga gacggaagga tcagctgaga ctcgacgatg ctgaacacca gggcgatgat
    23341 gacgacccag cccacgacgc tgaagaagcc ggccatccgg ccggtgctga gcagcagggg
    23401 caggaaggcg gccatcgtgg tcagcacccc gaagatcacg ggaatcgaga cctcccaggt
    23461 gccgtcgatg gccgcctgca ccggcggctt gcccatctgc tcgtgaccgt atactcgctc
    23521 gccgacgacg atggcgtcgt ccaccaggat gcccagaacc aggatgaatg ccatgacggt
    23581 catggcgctc atggagatgt ccgcataggg aaacacggca agcgtgccga gcagcgcgat
    23641 ggggatgccg gccgccaccc acatggccag cctgaatttc aggaacatgg cgaggatgat
    23701 gaccacgagc ccgagcccgg aaagcgccat gccgttcagc gtgtccaggc gggccttcag
    23761 ctgctcggac tcgtcgttcc acaccttcac gaacagcccg gccggcagcg tcttctcgta
    23821 gcgggtcaag tacgctttga tgtcggccgc catctcgagc gcgtcttcct tgccaacctg
    23881 ccagaccttg accacggcgg cggggtatcc gttgaagcgg gcgcgcatgt cgccctcttc
    23941 gaattcgtcg cggatctcgg cgatctcgct cagcatcacc ttggtgccgt cgttgcgcgt
    24001 gacgaccacg atgtcttcga actcctcgcc ccagtaggcc tggcccttgg cccgcaggag
    24061 aatctctccg cccttggtct tgatggtgcc gccgggcatg tccatggacg tgttgcggat
    24121 cacccgggcc acctgctcca gggtgatgcc gtaccggcgc agcgtatcct ccgatatctc
    24181 gatggagatc tcgtagggcc gcacgtagtt gagcgcgatc ttggagatgc cgtcgatcat
    24241 ggatagctcg tcgcgcagct ccttgccgag ctctttgagg gtgcgctcgt ccgtatcgcc
    24301 gtagacggca atctgcagga cgtcgctggt gatcgtcagc ttcgagacga tgggcttttc
    24361 cgtctcgagc ggaaacgtgt tgatgccgtc gacctggctt ttgatctcgt tcagcgcttc
    24421 cgtctggttg gcgtcctcgt agagcatggc ctgcacgctg caggagccct cgcgcgcggc
    24481 ggacagcaac ttctcgatgc cctcggtgcc ttcaagggcc tcctcgaccc ggatgcacac
    24541 gccttcctcc acctcctcgg gcgccgcgcc gaggtagggc acggtgacgt tcacgacgcg
    24601 aacgtccatg ctggggaact cttcctgatg gacgttgaaa agcgccaggc acccgccgat
    24661 cagcaggacg aacatcagca ggttcgcggc aacggggttc ctcgtgaacc aggaaatcgc
    24721 ggtgtacatg ggctcagccg gctgtcgggg cgttctctgt gacgggttgg accggcatgc
    24781 catcgacggc ggtctgcagg ggagatacgc agacgcgctc gccggcggcc aaccccgcct
    24841 tgatcagcac ctcgtccttg tacaggcgca ggggctcgat atcccgatag cgcaggttgt
    24901 ttccgccatc caccaccagc actcgatcgc cgtcgcgcag cgccgttctc ggcaaaacga
    24961 cgatgttatc ggccagcagc ccctcgatct ccgcgttgac gaacaagccc acgttgagcg
    25021 gcaccggctg ctccacgttg gaaatccggg cgacgacgtg aaccatgcgg ctcgccgtgt
    25081 cgatctgggc ttcggttcga acgatggttc cctgccattc caggctgcgg ccggcgtact
    25141 cggcaagcag cgtaacccgc ggctgctgct cggcgggaag ctcgccgcgg tgatccacgg
    25201 gcaggttgag gaacgccagc tggcggtcgg cgatgggcag ccggacctcc gcctggtcgc
    25261 tggcataaac ggtcgccacc ggagagccgc ggctgacgaa ttgcccgacg tccacggctt
    25321 cgcgtctaac caggccgtcg aagggggcgg tgagggcggt tcgttcgagg tcgcgcatcg
    25381 cctgctccat ggcggcgcgc gcctcctgga gggatgcttc ggcaaccctg aacgcgcgca
    25441 gggagttctc catctgggat ttgctcgcca gctggcgtac ctccaggctc ttcagccgct
    25501 ggtactcgta ctgcgcgtgc tcgaactccg ccgcggccct gtccagatgg gcttccgccc
    25561 tcgtcagcgc cgagcggtag tcggtggctt ccaggcgcag aagcacgtcg ccctgctcga
    25621 agtaacctcc attgaccagt gacggcgaga tccactcgac gcgtccggac acttccggga
    25681 tcagctcgct ctccatgttc ggtaccacgg taccctggga gtgcacggtc aggcggaccg
    25741 gctggggaat cacctccacc actcgaaccg tggtggccag gggctctatg gcgttgggct
    25801 ccagctcggg ggccgttgcc atcagcgtta ccgctccgaa gagggatgcc aggatgacga
    25861 gtcccggaac gagcagcttc ttcatcatga cgcggctacc cccgtgagcg gtgcgcggtt
    25921 ggccgcgtcg taggcttcga aggaccgtag aacggattcg cgcgtgagcg cagtgctctc
    25981 ggtgaacacg tggcgcagag cgtcggcgtc acgtttggcc agggcttcgt ctagtcgccg
    26041 ggcgttgtcc gcatgggcct ccgtgtcgaa ctgggcgaat gccttaagcg gctcggttcg
    26101 cggcgtgagc tgtgccagca ggctgcgggc gatgagctgg catacgagat tatcgctggc
    26161 gctcatgatc gcgcccatca gctccaggcg agcctccatg tgggcgaggt cgtccagatc
    26221 ctcctcgaag agcggtcggt tgtgttcgcg caggcgctcg atgtcttcgt cggtggccgt
    26281 attcagcgct gactccaccg caagcctgac aagggcggaa atgacttcaa tgatgtgaat
    26341 caccagcttg ctgtcaggaa ggtcgcccag ggccagcatg tggccgatca cgtccaggct
    26401 ggcttcttcg aggggcgcca cccgcgcgcc gccgggctgg atgttggcga tgccgagctg
    26461 ctccagtttc ttcatggcct cccggacggc gccgcggttg gcctcgaagc gcacggaaag
    26521 atcccgttcc gagggcagcc gctcgccggt gcggtactgg ccgctcagaa tgtccctggt
    26581 gaggctctcg gcgatttcct cgtgctttgc catgttcttc ctgttctgac tggtgcgacc
    26641 atgttggtct gaccatgatg gtcggggcat tttagtcaat caggttcgca tgtccagcat
    26701 ttcgttacct ttggtaacga aatgtgcgcg cgttcgcgaa accgcggcca gtggccgcgg
    26761 tggggatggg cggcggtcag cggccctgag gagagcggcg gtgatccagg acgttgccgt
    26821 ggcgcagcaa ctcggcgacc cgggcgctgc gcccggtcgg tttgctcgac cagtaggcgg
    26881 cgtcgaaggc cggcgcgcga cgtcggtgga tgatgcccat ggccaccggg ttcggtggtg
    26941 ccagggacag cagcatgtgc gccagggcgg tattggtctc gtcgtggacc accagatcgg
    27001 cctcgctcac gccgttctcg ccgagcgtca ccgcttccag cgccagggtc tgcggattga
    27061 ggcgcaaccc cttgtcgcgc tccttgccga acacgagcgg atggccgtgc tccacgacga
    27121 cctgagtgtc cgccgccgtt ttcttggcga ccacgtcctc gaagacgtct ttgttgtaga
    27181 cgatgcagtt ctggaggatc tccaccagcg cggcgccttt gtgttcccgc gccgcgttga
    27241 ggattcccgg caataccttc tgatcgacgt ccacggctct ggcgatgaag gtggctcccg
    27301 caccgacggc gaacaagccc ggttgcacgg ggctgtccac ggatcccttc gggctggaag
    27361 gacttctcgt gccgatgcgc gacgttggcg agtactgccc tttggtcagg ccgtagatct
    27421 cgttgttgaa tagcaggata ttcaggtcca cgttgcggcg aagcgcgtgc attaggtgat
    27481 tgctgccaat ggagagggcg tcgccgtcac ccgtgatcac ccagacgtcg aggtcggggt
    27541 tggccagctt caccccggtc gcgacggcgg gcgctctgcc gtgaatggtg tgaaaaccgt
    27601 aggtgtccac gtagtaggga aggcgtgacg agcagccgat gcccgacacg aataccgtct
    27661 gctctttggc ggctccagca tcagccagcg cccgctgcac ggccttgagg atggagtagt
    27721 caccgcatcc cgggcaccag cgcacttcct ggtcagtcgc gtagtctttg aaagtcagct
    27781 ccgccatgat tcaacctgcc tccttgatgc gcttgggcgc gtagcgcgcg atcgcctgct
    27841 tgacgtccgc cacggcaaac ggctgcccgt tgacctgatt gtgttgctcc accgggagga
    27901 ccagcttgtc tcgcagcagg gtcgcgagct ggccgttgtt cagctcggga acgaggatcc
    27961 ggtcgaactg cgccagcagg gtcggcaggt tcggcggcag cgggttcaag tgccgcagat
    28021 gtatgtgacc cacggccagc ccctcggcga cgcactctct gactgcctga tagatcgttc
    28081 cgtacgtgga tccccaggcc actacggcca gcccggcatc ggttcccgaa tccacctgct
    28141 gatcggggat gaagctggcg acgctggcca ccttggcctc gcgcatgttg gtcatttcct
    28201 gatgattctg agcttcgtag gagatgttgc cggtgtggat gtccttctcg atgccgccga
    28261 cccggtagac gaagcgtttg tccccgggcg ttgcccacag acgggcctcg gtcttcggat
    28321 cgcggctgaa aacggaatgg cgtggatcgg tgctgggatc cggtttgtgc gtttctatcg
    28381 gctcgtagtg atcgaagtcc ggcaggggcc aaagctcgga ggcgttggcg atgtagccgt
    28441 cggtcagaag aatcaccggc gtcatgtgcc gcagcgcgat gcgcaccgcc tcgatggcgg
    28501 tatcgaagca atccgcgggg gagcgggagg cgaccaccgg cagcggcgtg tccccgttgc
    28561 gaccgtacac ggcctggtaa aggtcggact gctcggtttt ggtgggcatc cccgtggatg
    28621 gccccgcccg ctgggaattt acgatgatca gcggcagctc ggcggccacc gccaggccca
    28681 tggcctcggt cttcagcgcg attcccggcc cggagctcga agtgacgccc agcttgccgg
    28741 cgtacgaggc gccgatggcc gagcagatcg ccgcgatctc gtcttcggcc tggaaggtcg
    28801 ccaccccggc atcctccagc ccggtcagct ggtgcagcag cgaggaggcc ggcgtgatgg
    28861 ggtaggagca gaacatcagc tccgtatccg cgagctcccc ggcggccacc aggcccaggg
    28921 ccagcgcctc ggcgccggtg agggtccggt actcggccga cgccagcgag gcttcgggcg
    28981 cggcgtgctg acgcagcttc gggccgagct cggccgtctc gccgaaagcg tggccggcgt
    29041 tcagagctgc gaggttggcg cttcgcactt gctcgttacc ggcgaatttc gcctcgatcc
    29101 aggagaccgt ggcgtcgcgt ttctgatcga acatccacag cacgagtccc agtgcccaga
    29161 agttcttgca gcgcagtgag tccttggcgc ccagcccgag agcgtctaca gcctggtggg
    29221 tcagatccga gatgtcgatg tcgagcagct cgaaaccatc gagggtgccg tcgtcgaggg
    29281 ggttgctcgt caggtccgcg cgcttcaggc gctggtcggt gaaggcctgc cggtcgacga
    29341 cgaccagcgc gcccggcctc agatgggaca ggttgaccac cagcgcggcc gggttgaagg
    29401 caatgaggac gtcgggagca tcgcccggcg tggtgacccg gtcgccgccg aactggatct
    29461 gaaacgcgga cacgccgtac cgggtaccgg ccggcgcgcg gatctccgcc ggaaagtcgg
    29521 gcatggtggc caggtcgtgg ccggcaagag cggaggccgt ggtgaactgc tggccctgaa
    29581 gctggatgcc gtcaccggag tcccccgcaa agcggataac gtagctggtt ttttcctcga
    29641 ccatttgagg ctcccggagc ccgcccgcaa aaaaaggtgc ggaattgtac ttaattccgc
    29701 gcgcaagtgg agatccgatg ggacataagg ttatgtcctg actgcccctg caggcgccgg
    29761 atgattttgc ggttcaaatc ccttagagtg cgccgcttgc cacactctgg agcctgaagt
    29821 gaaagtcgta gtcaaaaccg acgagtacac catttaccag cgccgcgacg aacgttatgc
    29881 cgtgaaggac gccacgaagg cctgggtcaa tggagacgcg aaggccgcca tcctcgtcca
    29941 gcacgagctg attacggccg ccctgccggc cgcgccgctc gaagagcccg aagccgacgc
    30001 cggcgagccg gcggcggaag ccgcggccgc cgaagaggtc actggggccg aagagtcggc
    30061 cgcggaagag tctgaacccg ccgaggcggc tgctcctgcc gaggaagccg ccgcggccga
    30121 ggttgctgcg gaagacgaag cgggcgagcc ggcttcggcc gaagagggcg aagaggaacc
    30181 caaaccggaa tagcgctcaa tggtcaaggc cgtcgcggaa tttgaggtcg tttccgacta
    30241 tgagcccgcg ggcgatcagc cgacggcgat cgccggtctc gtcgagggtc tcgtcgacgg
    30301 cctgagccac cagacgctcc tcggcgtaac cgggtcgggc aagacgtaca ccgtcgccaa
    30361 cgtcatcgag gccgttcagc ggccgacgct cgtcctggct cccaacaaga ctctggcggc
    30421 acagctctac ggcgagtttc gggatttctt tccgaacaat gccgtggagt actttgtttc
    30481 ctactacgac tattaccagc cggaggccta cgtcccggct tccgacactt acatcgagaa
    30541 ggactcgtcg atcaacgcgc acatcgagca gatgcggctg tcggcgacca aggcgctgct
    30601 ggagcgtcgc gacaccatca tcgtggcgtc ggtctcggcc atctacgggc tgggtgaccc
    30661 tcagtcctac ctgcgaatgg ttctgcatct ggatcgtggg gatcgcgtgg accagcgcta
    30721 cgtgctgcag cgcttggcgg agctgcagta cacgcgcaac gacatggctt ttcaacgggg
    30781 ctcctaccgc gttcgcggcg acgtcgtgga catatttccc gcggagtcgg agaaggaggc
    30841 cgtgcgtgtg gagctcttcg acgacgagat cgaaaacatc tccgtcttcg atcctctgac
    30901 gggcgagatc gtcaaccggg tgcctcgcta cacggtcttt cccaagactc actacgtgac
    30961 gccgcgcgag cgcattcttg cggtgctcga agacatcaag gaagagttgc aggataggct
    31021 ggcctatctg cagaagcatc acaagctggt cgaggctcag cggctggacc agcgtacgcg
    31081 cttcgatctg gagatgatcc gcgagctggg ctactgcagt ggtatcgaga actactcgcg
    31141 ctacctgtcc ggccgcgagc cgggtgagcc gccgccgacc ctgttcgatt acctgcccaa
    31201 ggacgcgttg ctggtcatcg acgaaagcca cgtgaccgtt ccccagatag gtggcatgta
    31261 caaaggtgat cgttccagaa aggagacgct ggtggagtac ggcttccggc ttccttccgc
    31321 tctggacaac cggccgctgc gattcgacga gt
//
LOCUS       DBNHLM010000209        28457 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_30202, whole genome shotgun sequence.
ACCESSION   DBNHLM010000209 DBNHLM010000000
VERSION     DBNHLM010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 28457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 28457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28457
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_30202"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..757)
                     /locus_tag="AC16QF_09580"
     CDS             complement(104..757)
                     /locus_tag="AC16QF_09580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020800241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR6448848.1"
                     /translation="MATENAYGLWTLVVLNSAIFILFAFSFARPRSPRDWRSLGAFSA
                     FIIALFTEMYGFPLTIYLLSGWLQNKFPETDLFSHESGHLWHTLIGWELNPHFDPLHI
                     LSNVVIVAGFLMLSSAWRVLHEAQKHHELAVDGLYARIRHPQYVAFIMIMFGFLLQWP
                     TLPTLVMFPILVVVYVRLARREEKAVLEEFGETYRAYMEQTPAFFPARRRTALEGTQ"
     gene            complement(747..917)
                     /locus_tag="AC16QF_09585"
     CDS             complement(747..917)
                     /locus_tag="AC16QF_09585"
                     /inference="COORDINATES: protein motif:HMM:NF023095.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HIR6448849.1"
                     /translation="MLYFLLTEHRAHFFFQALPYVIFLLCPLMHLFMHQGHGRNRSGG
                     SDSTTPGRPDGN"
     gene            complement(1084..1446)
                     /locus_tag="AC16QF_09590"
     CDS             complement(1084..1446)
                     /locus_tag="AC16QF_09590"
                     /inference="COORDINATES: protein motif:HMM:NF013068.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR6448850.1"
                     /translation="MHWRRFLSGLKHRGLHGIELIVSDAQKGLRGACKAVFSRVQWAE
                     EALPQGFTVISFPASHRRRLRTTNLAGRLKEEMPRRTPMAELFPNEASCLRLVTAVVM
                     EIAEDWQTAEERYLVSGK"
     gene            2404..3324
                     /locus_tag="AC16QF_09595"
     CDS             2404..3324
                     /locus_tag="AC16QF_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448851.1"
                     /translation="MDERDNASAPEALLSARKVDQVRARSRLGRQIVDVLNTDVRDWY
                     ERHRRSNATTPLVWTLCYVLVLMAVLVILLPFDTVQVEGRFRLIKPLNFTASFATYLA
                     VMVIFVDYLRVSEGWKKFITWGISICIATAITCITVQAARGTTSHFNQDTPFDAAVSM
                     LMDIADPVNSIFVFILLLFALQSKIDVSPPTRLGIVYGIIIFLVGGVVGGLMVFKGQS
                     GVGVAPGGPGLPVLNWSTIGGDLRVAHFLGVHAIQILPIAGWIIGSLDVFGRSMRAKR
                     GLVVAVCAGYAFITAFVFMQALSGAPFVRI"
     gene            complement(3548..3669)
                     /locus_tag="AC16QF_09600"
                     /pseudo
     CDS             complement(3548..3669)
                     /locus_tag="AC16QF_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_061586541.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3862..4275
                     /locus_tag="AC16QF_09605"
     CDS             3862..4275
                     /locus_tag="AC16QF_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448852.1"
                     /translation="MAEYEFSEEDNKTFDSLSRALRRFALTFAAFAVLLAIMGIIWIA
                     TGMPNPPTDSSAVEGVPWLIIVVYVGIPATAALALMFMQPLDNLKRITTTEGQDISEL
                     LAALGDLNQSFGLLRIFVAVLFIVMIVRVAILIFG"
     gene            4361..4726
                     /locus_tag="AC16QF_09610"
     CDS             4361..4726
                     /locus_tag="AC16QF_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448853.1"
                     /translation="MAEYEFTAAENQSIDQLRTKLSSISIMLVVAGALLVFFALGGET
                     SQAIWYSAIPAIAFIVLGYVYYRPVENLKRVTSTHGQDIMIMMMAMNDLRIAFTISQV
                     IFVVLIVMAIVQISRMLGF"
     gene            5092..5850
                     /locus_tag="AC16QF_09615"
     CDS             5092..5850
                     /locus_tag="AC16QF_09615"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6448854.1"
                     /translation="MPDLSGKTFIITGGNGGIGLGMAEGIAAAGAGVAIWGRKEEKNA
                     EALEKLKAYGTPAFAYVCDVSDEMAVIETLKRSTDDLGRLDGLFANAGRGGVGKKFVD
                     MTLEDWRAVMAVNLDGVFLTLREAARVLIEQGEGGSLVAVSSTSAIHGAAGNEAYGTA
                     KTGLNGLVRALAVAFARYGIRVNSLLPGWTKTDLAMSSYQSEKFRTVTTNRTPVRRWA
                     DPAEFREIGAFLADPSLTFHTGQEVCVDGGYTVF"
     gene            6377..6844
                     /locus_tag="AC16QF_09620"
     CDS             6377..6844
                     /locus_tag="AC16QF_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6448855.1"
                     /translation="MSDDEYLRDVHEIRNLKHTYCELADRCCSSSDARLAADEIVQLF
                     LEDGELEVPTEYGGRQVGRAAIHAFWMQQSVVFSFADHLVFNERIDVNGIEARGRWKN
                     VIPATIFVEDKPVAQWILGNYEDEYVKVDGQWRIRSVKVSIKRVFGRDEAGVA"
     gene            7263..7478
                     /locus_tag="AC16QF_09625"
     CDS             7263..7478
                     /locus_tag="AC16QF_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448856.1"
                     /translation="MAYLFCGANSQTAQSKESFEFTYAELGLGSTVVTGGMALAFADS
                     WAHFCAHLFDQDGLVRERSRLTAWLDG"
     gene            complement(7491..8870)
                     /locus_tag="AC16QF_09630"
     CDS             complement(7491..8870)
                     /locus_tag="AC16QF_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012544.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR6448857.1"
                     /translation="MHDSPPIERHRSLGQRDVVMYTVSAILLLETLTAAASIGAAAIF
                     WWLFLGVVFFLPFALICAEMGCTYPEQGGIYAWVRDAFGGRWGARASWGYWVNTAVWL
                     PAIYILFAGIFAQLYAPGLSLAGQIAIGIGLTWLTVGLNVVTLDVGKWVPNLGAAVKV
                     LVFLTLIGAASYHVATQGFANPINLETIRPSWSDSVAFIPAIIYGMLGFELVSASSEE
                     IKNPRRNVPRGILISGLIIISLYTLATTAMLAALPADEIDLVEGLVATLHQFFASSGA
                     GEIIVSILGAGALYTFFTNGVTWAMGCNRAAAEAGIEGELPRFFALETRRGTPLGAAV
                     VMGAFSTGVLLLYGGLASNSEDLFWSLFAFSAVIFLLPYLGLTLAFLHARRHDAGRHR
                     PFAVPGGMAIARTCAYVCFAALAASIGLFIYTPGEGIVHPVLWGALVTLAIGELTIRF
                     GEHHPVRAG"
     gene            complement(8863..10233)
                     /locus_tag="AC16QF_09635"
     CDS             complement(8863..10233)
                     /locus_tag="AC16QF_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009395600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HIR6448858.1"
                     /translation="MPIQSRELPEELDRTRHLHPFTDYAAYARSGGRVYTRGEGIFIY
                     DAQGRELLDGMSGLWCTSLGYSQPKIVDAVTEQLQRLPYYNSFFNCSNDAAVAMANAL
                     VEVTPPHFNHVFFTNSGSEANDTNIRLVHRYFDLIGKPDKKIILSRENAYHGSTIAAA
                     SLGGMSSMHKQFTRLPYVHHVQQPYWFELGSGRSPEEFGRQAAQAVADRIDELGADNV
                     AAFIAEPVQGAGGVIIPPETYWPAVQRILDERDVLLISDEVICGFGRTGRWFGCDYFG
                     TRPELLTFAKAVTNGYQPLGGVMVGDRIADVLTTQGGEFAHGFTYSGHPACCAAGLAT
                     LELYHELDTLSYVADDIAPYWASRWSELAAHPIVGESRTLGMLAAVELVSDKSGHTRL
                     DSDGKAALFCRDEAIGGGLMVRAVQDSIISAAPLVSTREEIDLLIERLTQALNDTARH
                     YGINDA"
     gene            10322..11896
                     /locus_tag="AC16QF_09640"
     CDS             10322..11896
                     /locus_tag="AC16QF_09640"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     sulfotransferase family protein"
                     /protein_id="HIR6448859.1"
                     /translation="MVKTADLGALLRDGLGALNAGELERAGAICKQVLARQPNLVQGH
                     FLVGLVALEAKDRKTAFQAFGSVTKLDPAHSAAWAQLARLFMSEGQVNRADAALAEAT
                     QHEPNDPLVHDVIGTVYSLMGEYGLAGEWFEKACATRPDHPPFMLNLANNHVYHGRTP
                     QALALLERVVALQPDSPQAHWSLAGSRTARDDDHIRACARLVMGPGLHPRLQAFYYYA
                     IGKECEDLERWDDAFEAFARGAAARRSTVEFDEAAEIEMFAYLEQQFDESWLESASGG
                     HPDASPIFVLGQPRTGTTLVERIISSHSEVTSAGELQQFGLGIRRLSGHRDPKRFSTG
                     LFEASLAVDPKQLGGVYLDTTRRMRGDTPRFVDKLPQNYLCIPLILAALPNAKIVHLT
                     RDPMDACFASFKQLFADAYLHSYDQREMARHHARYRRLMDVWRERFPGRFLDISYEET
                     AADLEPNVRRLLAFLDLPFEEACVSFHSQASAVSTASAVQVREPAHTRSVGRWKRYER
                     QLEPMREALAGAGVVG"
     gene            12017..14380
                     /locus_tag="AC16QF_09645"
     CDS             12017..14380
                     /locus_tag="AC16QF_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6448860.1"
                     /translation="MPRPARRALRNPNFQLKPIAAAVATALASSAPAMAEQATVIEEM
                     VVTATKRDESAQEVPLAITALSGEFTRSSNINDVKDLVVFSPGVSGNSQDSFIDAISV
                     RGIRTQDFGVGGDPSAAFFKNDLYEGRNGSAVTSLFDMERAEILRGPQGFLFGRNSIG
                     GAFSVHTAKAEIADRASGYVEVDIGERDHLVVEGAVNLPINDRFAMRLAGYSSNEDGF
                     VKNRFNDEKLIEHEKYALRWSTAFATDRLSLDTMVEYEEREQSGSVYRAIDEGDIWET
                     FEAAIGEVELRGGPYDADSDQSGGDNDDADILTIGVKFEYEFDFATLTSNTGYKDHEY
                     YYNEDYDGTPLNLNNYRQDQEGDYFQQELRLTSKGEGPLSWYAGASYYKEEIDTLFEF
                     AGQEDFFCQYYGYYYNYGMTFTGCSDLYAYYGSPFTPSADGVLRETGRIVGDYSGWAA
                     YVDLTYELTQQWDFSVGLRYSDDEKDFTINVPTPDSDLGAYWAYTFATDGDITGKESW
                     DDLQTRFVARFRPNDDAMLYASYTEGFKSGGFGSFALVDADGVGAGGGDTDLSQSDGY
                     RPNAFQPEVADSYEIGYKGSLAEGRAQLSLTGFYYEYEDLQVVVFDGGAAAVENVGNV
                     EGWGVEGTANFLLGDYVDVYLGLSYLDTEATGLQSICGLEDAEGCEGSSLFWAPEWTG
                     SVVVNGNFPLEAGGALTGRVEGFWESERGGGFEGLKTTEIDSFFNLAVRVGYESEHNW
                     SANLYVENLTNEDTFDGLNNNGGVLPSHFFGPMRPRTFGVSFSYVWD"
     gene            complement(14392..15210)
                     /locus_tag="AC16QF_09650"
     CDS             complement(14392..15210)
                     /locus_tag="AC16QF_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR6448861.1"
                     /translation="MSNLALISRDVAEILPAAGTDEFAPSVVAMLRDQVNIDEACMIV
                     YPRDESPRIAHRETQKPGDEPNLDTFIKGPFLLDPYYVAAAREGRFGFFPLSHLAPTG
                     FKTSEYYRTYYRFSGIFDECGYLTPIGDGGFANLSLARADGNQPFESSTLRCLADLAP
                     LVTALTQHHWRLLPANPSTEQISLRTQLESALESFGESVLTARERQIINSILHGHTSK
                     AIAQSLAISVETVKLHRKNAYRKLAIRNQSELFYTFIRALRDCSDEYQGGDPLR"
     gene            15288..16040
                     /locus_tag="AC16QF_09655"
     CDS             15288..16040
                     /locus_tag="AC16QF_09655"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011536407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR6448862.1"
                     /translation="MSRPVVLIPCDVKRYGEYPFHCVGEKYINAVAHGAGAMPLLMPA
                     WGRGAELRELTDLFDLDAILDRVHGVFLPGSTSNVHPHRYGGADLDMLHDEQRDAVVF
                     PLIERALSRGLPLLAVCRGLQELNVALGGTLHPRIHEVPGYLDHRENDELPRDAQYGL
                     VHAVDAVHGGMLADLVGAKRFLVNSLHAQGIAELGAGLRVEARAEDGLIEAVSMVGGW
                     VLGVQWHPEWHFTDDTASAKLFAGFGEALRAA"
     gene            16075..17388
                     /locus_tag="AC16QF_09660"
     CDS             16075..17388
                     /locus_tag="AC16QF_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR6448863.1"
                     /translation="MGKPSEMPDELELLLSDMNGVPRGKTLRGISYSEAHPPHLAEAI
                     FFQTITGGYAAAMETYDPNDRDLLLRPDWSTYVPTPWKPDTGQVICESLDQAGERLPY
                     DPRNVLRRVLDAYESMGLRPVVAPEVEFYLLEAVSSHDLVLTPATGRSGAAEFGGESF
                     SPDALEKFMPIVAEIQQACRQTGLELSAMVHEMGPAQIELNVAHGDALSRADQLFQLK
                     RLIKGCARKHGALASFMAKPLADLPGNGLHLHCSMEDETGRNIFALNRRRAPAVLQHF
                     IAGLQAHLPQAFALVAPNVNSYKRFVRDLSAPINLEWGYDNRTTGFRVPFSDARAGRV
                     ENRVAGADANPYLIIAATLACGLLGMRAAIKPTKPIADDAYGLKPALPEDLGEALRIL
                     EQSEGLIDLLGEAFVEVFVSVKRGELDHFGKTITQWEVGYLGSTL"
     gene            17460..18692
                     /locus_tag="AC16QF_09665"
     CDS             17460..18692
                     /locus_tag="AC16QF_09665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6448864.1"
                     /translation="MRTYPGLDGSCEADVCIVGGGYTGLSAALELGRRNYDVVVLEAH
                     RVGSGASGRNGGVLGMGQRKDQLELESWLGADAARKLWRIACDANDLVRDRIAEFGID
                     CDLKSGELTAAHRERYEKTLWSYAEHLERDYGYSDRRIVSREEVAKMLGADTLYGGYV
                     DLRAGHVHPLNLAIGLARAASDHGATLHEQTPALGYRQEGDRVLVRTASGDVRARFLI
                     IACNGYLGRFEKGAERYQMPINNFILATEPLGAEMGRAINRDDLAVVDTRFVVNYFHN
                     SPDHRLIFGGGENYSSRFPKDIKAFVRRRMLQIYPQLESARIDFGWGGTLAITMRRMP
                     QFGRKGANVYWAQGYSGHGIAMANMGGKLVADAIMGQAEEFDTFAGLKHLPFPGGRYL
                     RWPALVSGMLYYAFLDRL"
     gene            18694..19632
                     /gene="speB"
                     /locus_tag="AC16QF_09670"
     CDS             18694..19632
                     /gene="speB"
                     /locus_tag="AC16QF_09670"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008369387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HIR6448865.1"
                     /translation="MTRSEHTRSDLLGHRSGMTFAGELSFLRRKYTRDIEGADLVVTG
                     LPFDMATTFRPGARLGPQAIRAASVQLAGIAGRAFPYGEDPFATLAVADCGDCFIDSG
                     YPQHGAAAIEAHARDIIARGCKLLSFGGDHFVTYPLLRAHAEKHGPMSLIHFDAHVDT
                     WEDDGSRIDHGSMFLRALREGLIVPERSVQIGIRSFSQQDHGFVVCDAPWVHEHGPQA
                     VLERVLSVVGQHPAYLTFDIDCLDPAFAPGTGTPVAGGLSSAQALHIIRGLTPVNLVG
                     MDVVEVAPAYDHAELTAIAAATIAHDLICQEALRRG"
     gene            complement(19668..20768)
                     /gene="ald"
                     /locus_tag="AC16QF_09675"
     CDS             complement(19668..20768)
                     /gene="ald"
                     /locus_tag="AC16QF_09675"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR6448866.1"
                     /translation="MRIGVPREIKIHEYRVGLTPAAVAALTAAGHQVVVEHDAAAAIG
                     FSDADYRRAGAAVGSAKETFAAELIVKVKEPQLQECAMLCDGQMLFTYLHLAADRPQA
                     EALMRSGATAIAYETVTSPAGGLPLLTPMSEVAGRMSIQVGARALEMASGGRGTLLGG
                     AAGVAPGRVTILGGGVAGTNAATMALGLQAEVTILDRSAQRLHWLRRHFNGRARVLMA
                     DGNAIGKAVETADLVVGAVLVPGAAAPRLVTREMVEAMANGAAIVDISIDQGGCFETS
                     RPTSHDQPTYVEAGVVHYCVTNMPGGVARTSTLALSKVTLPFVMELASRGWEDAFARD
                     RHFANGLNVHRGSIRHPEVAKALEHAVPPMAA"
     gene            20916..21374
                     /locus_tag="AC16QF_09680"
     CDS             20916..21374
                     /locus_tag="AC16QF_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009681892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR6448867.1"
                     /translation="MDRIDRAILRELQNDARLPNTALAERVGLSDSACLRRVRRLEQS
                     GVIRGYVALVDETLAGYPDSVFVQITLVSQQRKDLEAFEDAVARVPEVMECYLMSGAS
                     DYILRVVVADARDYERIHSQQLTRLPGVDRVHSNFALRTVIENTEIPIRT"
     gene            21517..23067
                     /locus_tag="AC16QF_09685"
     CDS             21517..23067
                     /locus_tag="AC16QF_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR6448868.1"
                     /translation="MEISDQSQLALIALCIYFVVVVGLGLFYSRKSQSTEDFILAGHS
                     LTTPFVMGSVVATWMGGAVILGGAKEAFVGGFQAIVWDPWSPMLTLILCGFFFVSVFR
                     RSRFTTSIDFYNARYNRGIGLAALSISMVAYISWISAQLLSLGVIIKVVTGMDAVMAT
                     LAGSMIVLVVSLSGGLWALSRSDMLAFIILSVVLILVLPLALDAVGGPAAFIENAGTR
                     DGSPPFSLFYTEAPTAAGEPTGFYGYLGMLGVFYMLAAWFSVAIGDLGGSVLTARALA
                     AKDEVSATRGFLFGGVIYLLLGMIPVIVGMCVFILSPDIAEADLDDVLPIFVQQYLPD
                     WITVLFFVAVASAIVSTAGDTILTGGALLGYTALGAVKPESSDRTRLRATRTAMVLFT
                     AIALVFGLAMGDLYRLLVFAGAVAFPVTSAAFVCGVLWKRANVPGAWASIAAGTVSWV
                     IFVVLLLPEVDWEVWDALYIGAVPAFLCSAIAIVVVSLATRRTHPPNPVRDVDGNDIS
                     GGPLFRWR"
     gene            23281..23712
                     /locus_tag="AC16QF_09690"
     CDS             23281..23712
                     /locus_tag="AC16QF_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6448869.1"
                     /translation="MGIPIRVLIVLLSALAIAGCQTAAPEGERSGYEYDGRNLYLGYC
                     ASCHGLSGAGDGPVAAGMAFKMEDLRTLEARNGTFPADGLREVIDGRTLRSVHGTNDM
                     PVWGWQFLLAEDSEEHVAARIDALVEYLRSIQHGDSDPAAP"
     gene            complement(23692..23946)
                     /locus_tag="AC16QF_09695"
     CDS             complement(23692..23946)
                     /locus_tag="AC16QF_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448870.1"
                     /translation="MTEQPLIGPDDPPPFTVFNPEGSARVLFVSDHGGRAFPKAMEQL
                     GLADWVLERHVAWDIGSGDLAERLARQFDSPGRWSGRRGI"
     gene            complement(24016..24849)
                     /locus_tag="AC16QF_09700"
     CDS             complement(24016..24849)
                     /locus_tag="AC16QF_09700"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448871.1"
                     /translation="MNNFEGKVAVITGGATGIGFSFAKRFGAAGAKVIIAGRRQQKLD
                     ESVAALQQLGYEAAGFVCDVSDLTQVEALADFAEAKFGGADVIMNNAGILIPPSTVID
                     ADPKLVRQLLDVNYMGVWNGASVFGKRFIERGTPAAIYNVGSENAFFNIVPGMFAYES
                     AKHGLHSMTDALREETPDFIDVSFICPGFVQSEMTAMAGDIPMDVEEYTSIAWPQILN
                     GEYYVVSHPYNVVWIQHRYDEMMKAYETYAPRYDGDWQYDPRAMAAKAAEAAKDDWRK
                     R"
     gene            25013..25663
                     /locus_tag="AC16QF_09705"
     CDS             25013..25663
                     /locus_tag="AC16QF_09705"
                     /inference="COORDINATES: protein motif:HMM:NF016240.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HIR6448872.1"
                     /translation="MNEARVLGCLMEKSVITPDQYPLTLNALTNACNQKSSRDPVLSL
                     EPGVVQRTARDLEAKHLIASDENFKSRVEKFSQRFCNTPFADVQLDSAEFAVMCLLLL
                     RGPQTPGELRSRSGRLHDFADNQAVVETLQGLLEREGEPLVARLPRKPNRQDHEYAHL
                     LSGTIESMPVAEAAAHVRAPGRTAELEGRVAALEQEVRELREALQQMRAEREEEKS"
     gene            25660..26499
                     /locus_tag="AC16QF_09710"
     CDS             25660..26499
                     /locus_tag="AC16QF_09710"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016968880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448873.1"
                     /translation="MTDWLKAGNNAVITGGASGIGLAAATHYLSAGMNVLIADLDEDA
                     LSTAAASLADSGSGTLHTQVCDVSDFAQVETLQATAAAKLGNVHCLMNNAGAGFPPSA
                     PWENLDVWKKQVEINLWGIIHGCQAFVPAMLEAGERGAIVNTGSKQGITNPPGGYAYN
                     LSKAGVKNYTESLAHALRQVEGCQLSAHLLVPGFTYTGMISRFIPEKPAGAWTPDEVV
                     AFLIRSLDRGDFYVLCPDNDTPRDLDEKRIQWNTDDLIRNRSALSRWDPKYKDAYEAF
                     VSD"
     gene            26875..>28457
                     /locus_tag="AC16QF_09715"
     CDS             26875..>28457
                     /locus_tag="AC16QF_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448874.1"
                     /translation="MGRNGEGRAPRATRGFQAGCLSALLFLGACTGSSEKIFEQSEEL
                     SLRGSVGDGPIVGAQIVVEDAEGQMVFTAESDAAANYQIDVPDGTDLPVTIRVSGGTD
                     LVTGRGADFELLAVAFTSGDLTVNASPYTTLAVKTAQCMGGLSEANLATAWQYIDANM
                     NLGWDGEIIPNPMNEPIADDNIATVLVGNEALGEVIRRTSAALAPTTEALSTDEVIQM
                     LACDIADGQLDGVGPDVSPRVALTLLGTATAVRVEVISGALFVDGQDATPLLDSALQT
                     VLPGSLRSVTELAPNQQLIDQTLSGLAVLGTFDFAEATTLAIALEGATPDTARGQLAP
                     ALTSSVTTNLQDLGSQLALADTTTIEELLLRQADSAGAPVPVVSFDAEPSSVEAGTST
                     RLSWAASDADECLASGGWSGSQATSGTFQTPLLEQSTEYVLSCVGLGGTGTGRVFVEV
                     TQPAPVPATDAGSDPDPASDPDPVSDPISDPDPISDPDPISDPDPVSDPEPEPEPEPA
                     PAPAPPPAPPVVSISADRTT"
BASE COUNT         5278 a         8587 c         9072 g         5520 t
ORIGIN      
        1 cgggaaccgt cagatgcgct gacgccagtc gattggtggc ggctgcttca gattcggcca
       61 tgagagttat ccttcttcat ttgatcgctg agccaggtcg ctttcattgt gtgccttcca
      121 aggcagttcg tcgacgtgcg ggaaagaagg ctggcgtttg ctccatatag gctcggtatg
      181 tttctccgaa ttcctcaagc accgcttttt cttcgcgccg agccaaacgg acgtagacga
      241 ccacgagtat tgggaacatc acgagcgtcg gcagcgttgg ccactgcaga agaaacccaa
      301 acatgatcat gatgaatgcc acgtactgcg gatgtcttat ccttgcgtag agcccgtcca
      361 cagcgagttc gtgatgcttc tgcgcctcgt ggagcacccg ccaggcagac gaaagcatca
      421 ggaaaccagc gacgatgaca acgttgctga ggatatgcag tggatcgaag tgagggttga
      481 gttcccaccc gatcagcgta tgccagaggt gtcccgactc gtgactaaag agatctgtct
      541 caggaaactt gttttgcagc catcccgaga gcagatagat ggtcagcgga aatccgtaca
      601 tttcggtaaa caaggcgatg atgaaggctg agaaagcccc aaggcttcgc cagtctcgcg
      661 gtgaccgcgg gcgtgcaaag ctgaaggcaa aaagaatgaa gatcgccgaa ttgaggacaa
      721 ccaatgtcca cagaccatag gcgttctcag ttgccatcag gtctgcccgg tgttgtggag
      781 tctgatccgc ccgaacggtt gcggccgtgt ccctggtgca tgaacaggtg catcaaaggg
      841 caaagcagaa aaatcacgta cggcagcgct tgaaaaaaaa agtgggcccg atgctccgtg
      901 aggagaaagt agagcattgc ggcgaggaaa gcgcctgcgg ccaaaccgcg acccgacaac
      961 caaaaactgc ctaggctgtt ttgttttctt ggctcattcg tccgttggtc atccttcacg
     1021 aagaatctcc gcccagatta agcaacatct cttctgtaat ttagtcagcc tttcgaggct
     1081 tcattacttg ccggacacca gatagcgttc ctccgctgtc tgccagtcct cggcgatctc
     1141 catgacaacg gcggtcacta ggcgcaggca ggaggcctcg ttcgggaaca gctcggccat
     1201 gggtgtccgt cttggcatct cctccttgag gcgccccgcc aggttggtcg tgcgcaaccg
     1261 gcgccggtgg ctggccggga agctgatcac ggtaaacccc tgtggcagag cttcctccgc
     1321 ccactggaca cggctgaaca cggccttgca agcgccccga agaccttttt gagcatcgct
     1381 gacgatcagt tcgataccgt gcagcccacg gtgcttcaga ccggacagga accggcgcca
     1441 gtgcaccccc atagggcccc gggcccagat tctccgagcg ctccgccagc atggccgcgt
     1501 tcagcagcac gctgaccgct tccgccaggc cgtcgaagcc attttccttg agcagttcca
     1561 tcgcgtcatc gatcgatctg ttctctgtgt gctgagccat tttggtctcc tctcgtctgc
     1621 aaatcggcaa gttcgcgtcc gaaaggatga ccgggtggct cggcattaca tccgcccgga
     1681 acaattaatt aagattccag aaggaagggt gctctaactc cgcccaatca gcctgtttga
     1741 atcttgcgcc cgacgtatca acggttcaag tcaaagcatc gcttggtcgg ctacgtgatc
     1801 cccactgtgg ggattttgcg ggaatcagtt atgtatcgtt gtgccttgtg gggcatttgg
     1861 ttgcaacgag cgcctcggta actgtttgat ttaattggtt ttatctattc gctgtcgcta
     1921 cagttaatca gcggcttcct ggccaggaag ccgcgggcgc ctgccgtgtg acggccggtc
     1981 gagcccagca ggcggagcca tctaagaatc tgaattcgtt ggtgttttct aagcaactgc
     2041 tgaagcactg gcacaagggg agctgctaga tggggtccct cggttgggct aaaccggccc
     2101 ggaggcaagt aaggtgccga ccaccatgaa attgccttgc gtgcatgtcg ttttacgcaa
     2161 agccatcata gccatctcct tcctgaacca tgcaggtttc ggttttgccc cggaccctgc
     2221 ttcggacgtt gtatcctggc ctcacgcctg acttatcctc cacaaccgat gccgcgcaca
     2281 cggcgaattg ccggccggcc caatcggctt gcacgccagg cccatccttg tcacactcat
     2341 ctgctgagcg tggctgcacg tgtggattcg tagtagggtc ggccaacctc gcggagagaa
     2401 ctaatggacg aacgggataa cgcatcagct cccgaggcat tgctgagcgc tcgcaaagtc
     2461 gatcaggtcc gcgcgaggtc gaggttagga agacagatcg tggatgtact gaacacggac
     2521 gttcgcgatt ggtacgagcg acatcgacgc agtaatgcga ccacccccct cgtctggacg
     2581 ctttgctatg tattagtctt gatggctgtc ctggtcattt tattgccatt tgataccgtc
     2641 caagtggaag ggcgatttcg cctgattaaa ccactcaact ttacggcatc cttcgcgact
     2701 tatcttgccg tcatggttat ttttgttgat tacctccgtg tctctgaggg gtggaagaag
     2761 ttcatcactt ggggaatctc aatctgtatt gccaccgcca tcacctgcat cactgtgcaa
     2821 gcggcccgtg gtacgacttc acacttcaac caagatacgc ccttcgacgc ggccgtatcc
     2881 atgctgatgg acatagcgga tcccgttaat agcatcttcg tctttatcct gctgctcttc
     2941 gctttacagt cgaaaatcga tgtgtctcct ccaactagat tgggaatcgt gtacgggatc
     3001 atcatttttc ttgtgggagg tgtcgtcggg gggctgatgg tcttcaaggg gcagagcggg
     3061 gtgggcgttg cacccggagg tcccggattg ccggtgctca actggagcac catagggggc
     3121 gatctcaggg tcgcgcactt cttaggtgtc cacgcaatcc agattttgcc gatagcgggc
     3181 tggataattg gctcactgga cgttttcgga aggtcgatgc gagcgaagcg gggactagtt
     3241 gttgccgtct gcgcagggta tgccttcatc acggcctttg tgttcatgca ggccttgagt
     3301 ggcgcaccgt tcgtgagaat ttgaattcag aggggatgtt tactttgact gttccccgtc
     3361 accccatgtt ggacaacaga cactgattca gtaagagaca cttttggctc atcgttaatc
     3421 gcacagagga acgatgatga ccaaagggga ccatgaccgt gccgagaaac cggtacgcga
     3481 tcccgccgag ccacctagcg taaacacgat cctgagcaga agatccgcct cgtgccggcc
     3541 ggcaagaggc gaacacccca tgaaagcggg tgaggttgag cccgggccga gcaccagcgc
     3601 ggcgagacga gctatgaagt ccggtggatc gaggacgaca tgacgggtgt catcgcggaa
     3661 cagctgcttg aacgtctaga cgatctgccc gcgttcgttg gttgagaggc ggggggacct
     3721 tcagatagac tttccacggc ccctgaaatg gcttgtatcc tggggtttcc acgggaccat
     3781 gagttcattg cagactcgat ggggaacatg tacaggctcg aaacacctct aattaaatga
     3841 cgcaccacaa gggggcgaga agtggcggaa tatgaattca gcgaggaaga caacaagacc
     3901 ttcgattcat tatcccgcgc gctgcgacgc tttgctctga ccttcgctgc cttcgccgtt
     3961 cttctggcga ttatgggcat catttggata gctactggca tgcccaatcc gcctaccgat
     4021 tcgtctgctg tggagggtgt gccctggcta attatcgtgg tttatgtggg gattcccgcg
     4081 acggccgcac tggcgctgat gtttatgcaa ccactcgata acttaaagcg gatcacgacg
     4141 accgagggtc aggacatttc cgagttgctc gcggcgcttg gtgatctgaa ccagtcattt
     4201 ggtctgttac gtatattcgt cgcggttctc ttcatcgtga tgattgttcg ggtagcgata
     4261 ctaatattcg gttaatcacc agggatggtg gctgctcaag tcttgcaatt gagcgtcatc
     4321 tccaggggga ccacgcaaaa ggtcaatgag aggaatgatc atggctgaat acgaatttac
     4381 ggccgctgag aaccagagca tcgatcaact tcgcactaaa ctgtcgtcga tatcgatcat
     4441 gctggtcgtg gcgggagcgc tgttggtatt ctttgctctt ggcggcgaga cgtcccaagc
     4501 catctggtat tcggcaattc ccgcgatcgc ctttatcgtt ctggggtatg tctattaccg
     4561 gccggttgaa aatctgaaac gcgtcacctc aacccatggt caggacatca tgattatgat
     4621 gatggcgatg aacgatctgc gaatcgcctt cacaatatcg caggtcatat ttgtcgttct
     4681 aatcgtcatg gcgatcgttc aaatcagccg gatgcttgga ttttgaggac aacgacggcg
     4741 ctttatgcat cggcaagtct gaaggtctgc caagtatcga tttggccatg aaactcgtgc
     4801 tcggatacca acgccgaatc tacatctcga ggttctgagc ccgtcgcgtt ggtccgtcgc
     4861 tcgaagatga gatccactgt acatataagc gggtataaga agaacaaccg ccggaaacat
     4921 ggtggtgatg aactgcggcg atggtgtctg ctctgttgct actcctgccc gttcaggcag
     4981 ccgttctctg tcccagccat cgccaggtga cctcagattt agctattctt ccggtcgcgc
     5041 cagcggggaa ccaacagatg ctcaatgacc gtccaggagg agatgaccgg gatgcctgat
     5101 ctcagcggga agactttcat cattaccggc gggaacggtg gcataggcct cggcatggcc
     5161 gagggcattg cagctgccgg ggcaggcgtc gccatctggg ggcgcaagga ggagaagaac
     5221 gcggaagcgc tcgaaaagct caaagcctac ggaacccccg cgttcgccta tgtctgcgac
     5281 gtcagtgacg agatggctgt gattgagact ctgaagcgat ccaccgacga tctcgggcgg
     5341 ctcgatggcc ttttcgccaa cgccggccgc gggggcgtgg gcaagaaatt cgtcgacatg
     5401 accctcgagg actggcgggc ggtcatggcg gtgaacctgg atggggtgtt cctgactctg
     5461 cgcgaagcgg cccgggtgct catcgagcag ggtgagggcg ggagcctggt ggccgtctcc
     5521 tccacttcgg ccattcacgg ggcggcaggc aacgaagcct acggcacggc caagaccgga
     5581 ctcaacggcc tggtccgcgc cctggcggtg gcatttgccc gctatggaat ccgggtgaat
     5641 tccctgctgc ctggctggac aaagaccgac ctggcaatga gcagttatca gagcgagaaa
     5701 ttccgcaccg tcacgaccaa ccggacgccc gtcaggcgct gggcggaccc cgccgagttc
     5761 cgggagatcg gcgcttttct ggcagatccg agcctcacgt tccacaccgg acaggaagtc
     5821 tgcgtggacg gcggctacac ggtgttctag cccgcatggt ccgcccgact gggttgaatc
     5881 gcccgtgctg ccagctgctc cgtttcgcgc ggattcatgt cgagtcggct ccttggtgga
     5941 cgggttgatg atcatgctcg caaaccttta gtttcagcac cggtcaatga gagccgtcag
     6001 agccagttgt ggaacatggg ctgatcggga agagggaatg gccctgatcg gtgaggatct
     6061 gagctggatt ctcggagaaa gggcttctaa ccttcctgat gagtggtgcc cggcagccga
     6121 tctcagcaag caccagtgga gatcatgctg ccgagatgcg catcagtggt ctgtggctac
     6181 acgaacggaa tccgctactt ctaggatgtg gcgtttagtt ggggcgcgcc tactcttgcc
     6241 tgataggcca acgacgtgtg gtcggcatga atcgcgccca catgcctgga ttggcatttg
     6301 ttgcctcgat tccatcgaac gttgccacaa tgttttctcg ccgaagaata cggttcccaa
     6361 ggagggctga aacgccatgt cagacgacga gtatctgcgt gatgtgcatg aaattcgcaa
     6421 cctcaagcac acctattgcg agttagccga tcgttgctgc tcatcgtccg atgcccgact
     6481 ggccgcggac gagattgttc agctgttcct ggaggacggt gagctggagg tgccgaccga
     6541 gtatggtggc cgacaggtcg gtcgggccgc gattcatgcg ttctggatgc agcaatccgt
     6601 ggtgttcagc tttgccgatc atcttgtgtt caatgagcgc attgacgtga acggcattga
     6661 ggcccgtggt cgatggaaga acgtgattcc ggcgacgata ttcgtcgagg acaagccagt
     6721 tgctcaatgg atactcggta actacgaaga tgaatacgtg aaggtcgacg gccagtggcg
     6781 gatcaggagt gtcaaggtga gcatcaagcg tgtgttcggc cgcgatgagg ccggagtagc
     6841 ctgaagcgat attggtggat tgaccgtcaa tattcgctgg aagccggatc cacaatagct
     6901 ggctcggccc gttagacaga tgctgagaat cggtgactgt cgcaaacggg ccgttccgcg
     6961 atcgggaaga agaggcagac cccgctcggc aatcccaagt tcacggcaca cttcgcccgt
     7021 gatcatgccg ccggccagct cgatacccgt ctcccccagc atgtggatgg tcggctccgg
     7081 cttacactgc ttcctgccca tatccctggc ctgtcgtgag tgaaagccgc tctcaccaat
     7141 tagagtcggt gcacgggcgc aggtcatatc cgctggcccc gcagcttgcg taccccgccg
     7201 ctcatgaata cgcgttggcc gtctaaatga tcggcttcgc gcgccatcat gcgagctgac
     7261 cgttggctta cctgttctgt ggagccaatt cccaaaccgc gcaatcaaag gagagcttcg
     7321 agttcaccta cgccgagctt ggtctgggct ccaccgtggt caccggcggc atggctttgg
     7381 cgttcgcgga ctcatgggcg cacttctgtg cgcatctctt cgatcaggat ggcctcgtcc
     7441 gggagagatc gcgactcacc gcgtggctgg acgggtgaag ccggctcggg ctagcccgct
     7501 ctcaccgggt gatgctctcc gaatcggatg gtcaactcac cgatcgccag ggtgaccagc
     7561 gcgccccaca gcaccggatg gacgataccc tcgccggggg tgtagatgaa caggccgatg
     7621 gatgccgcca gggcggcgaa gcacacgtag gcgcacgttc tggcgatggc catgccgcct
     7681 ggcacggcga agggccggtg gcggccggca tcgtggcggc gcgcgtgcaa gaaggcgagc
     7741 gtcaaaccga gatagggcag caggaagatc acggcgctga acgcgaacag cgaccagaag
     7801 agatcttcgc tgttgctcgc caggccgccg tagagcagaa gcacgccggt gctgaacgcc
     7861 cccatgacca cggcagcgcc cagcggtgtg ccgcggcgcg tttccagcgc gaagaatctc
     7921 ggcagctcgc cctcgatgcc ggcctcggcg gccgcccggt tacaccccat ggcccaggtc
     7981 acgccgttgg tgaagaacgt gtagagggcc ccggcgccga gtatcgaaac gatgatttcg
     8041 cccgcaccgc tggatgcgaa gaactggtgc agcgtggcga cgaggccctc caccaggtcg
     8101 atctcgtcgg cgggcaacgc ggccagcatc gccgtggtcg ccagggtgta cagggaaatg
     8161 atgatcagcc cgctgatcag gatgccccgg ggcacgttcc tgcggggatt cttgatctcc
     8221 tcgctactgg cgctgacgag ctcaaacccc aacatcccgt agatgatcgc ggggatgaag
     8281 gcgacggagt cgctccacga cggccggatg gtctcgaggt tgatggggtt cgcgaatccc
     8341 tgcgtggcca cgtggtaact ggcggctccg atcagggtaa ggaagacgag gaccttgacc
     8401 gctgccccca gattcggcac ccacttgccc acgtccaggg tcacgacgtt cagcccgacc
     8461 gtgagccagg tgaggccgat gccgatggcg atctgcccgg ccagggagag gccgggagcg
     8521 tagagctgcg cgaatatgcc ggcgaacagg atgtagatgg cgggcaacca cacggccgtg
     8581 ttgacccagt agccccagct ggccctggcc ccccagcgac cgccgaaggc gtcccgcacc
     8641 caggcataga taccgccctg ctcgggatag gtgcagccca tctcggcgca gatgagcgca
     8701 aagggcagga aaaaaaccac gccgaggaac agccaccaga agatggcggc cgcgccgatg
     8761 gaagcggcag cggtgagagt ctccaggaga aggattgcag agacggtgta catgaccacg
     8821 tcgcgctggc ccagcgatcg gtgcctttcg atcggcggcg agtcatgcat cgttgatgcc
     8881 gtagtgcctg gcggtgtcgt tcaaagcctg ggtcagccgt tcgatcagca gatcgatctc
     8941 ctcgcgggtg ctgacgagcg gcgcggcgct gatgatgctg tcctggacgg cccggaccat
     9001 cagaccgccc ccgatcgcct cgtcacggca gaaaagcgct gcctttccat ccgaatccag
     9061 gcgtgtgtgg ccggacttgt cactgaccag ctctaccgct gccagcatcc ccagggtgcg
     9121 ggactctccg acgatgggat gcgcggcgag ctccgaccag cggctcgccc agtagggggc
     9181 gatgtcgtcg gctacgtagc tgagcgtgtc gagctcgtgg taaagctcca gggtcgccag
     9241 gcccgccgcg cagcaggcgg ggtggccgga ataggtgaat ccgtgggcga actcgccgcc
     9301 ctgggtggtc agcacgtcgg cgatcctgtc gccgaccatg acgccaccaa ggggttgata
     9361 gccgttggtc actgccttgg cgaaggtcag aagctccggg cgggtaccga agtagtcgca
     9421 accgaaccag cgccctgtgc ggccgaaacc gcagatcacc tcgtcgctga tcagcagaac
     9481 gtcgcgttcg tccagaatgc gctggacggc tggccagtaa gtttccggag gaatgatgac
     9541 cccgcccgcc ccctgcaccg gctcggcgat gaacgcggcc acgttatccg cgcccagctc
     9601 gtcgatcctg tccgccacgg cctgggccgc ctgccggccg aactcctccg gcgatcgtcc
     9661 tgatcccagc tcgaaccagt aaggctgctg gacgtggtgc acgtagggca gacgagtgaa
     9721 ctgcttgtgc atgctggaca tgccgccgag gctggcagcc gcgatggtgc tgccgtgata
     9781 ggcgttctcc cggctcaaga tgattttctt gtcgggcttt ccgatgaggt cgaagtagcg
     9841 gtgcacgagc cggatgttgg tgtcgttcgc ctcggagccg gagttcgtga agaacacgtg
     9901 gttgaaatgc gggggcgtca cttccaccag ggcgttggcc atggcaaccg ccgcatcgtt
     9961 ggagcagttg aagaagctgt tgtagtaggg aaggcgctgc agctgctcgg tgacggcgtc
    10021 gacgatcttc ggctggctgt agccgaggct cgtgcaccac agcccggaca tcccgtccag
    10081 gagctcccgg ccttgtgcat cgtagatgaa gataccctct ccccgggtgt agacacgccc
    10141 gccggaacgg gcgtaggccg cgtagtcggt gaaggggtgc aggtgccggg tgcggtcgag
    10201 ctcctcgggc aattctctgc tttggatggg catcgcgcgg gccttcccta gtgtgagcaa
    10261 tccgatggtg acatcattgc cgagttgttg ccttacccca aaagggggag tgagcaggga
    10321 gatggtcaag accgctgatc tgggcgcatt gctgcgggac gggctgggcg cgctgaatgc
    10381 gggtgagctg gaacgagccg gggcaatctg caagcaggtg ctcgcccgac agcctaatct
    10441 ggtgcagggc cacttcctcg tcggtctggt cgcgctggag gccaaggatc gcaaaaccgc
    10501 atttcaggca tttggatcgg tcacgaagct cgatcccgcg cactctgcag cctgggccca
    10561 gctggcccga ctgttcatga gcgaggggca ggtgaaccgc gcagacgccg ccctcgcgga
    10621 ggcgacgcaa catgaaccca acgacccact tgtgcacgac gtgatcggca cggtctactc
    10681 gttgatgggc gagtacgggc tcgccgggga atggttcgag aaagcctgcg cgacgcgccc
    10741 cgatcacccc cctttcatgc tcaatctcgc caacaaccac gtctaccacg ggcgcacgcc
    10801 ccaggcgctg gctctgctgg aacgggtggt cgctctgcag ccggactcgc cccaggcgca
    10861 ctggtccctg gcaggctctc ggaccgcacg ggacgacgac cacatcaggg cgtgtgcgcg
    10921 cctggtcatg ggcccgggct tgcatccccg gctgcaggcc ttttactact acgccatcgg
    10981 caaggagtgc gaggatctgg agcggtggga cgacgcgttc gaagcgtttg cccggggcgc
    11041 cgcagcccgc cgcagcacgg tggagttcga tgaggccgcg gaaatcgaga tgttcgctta
    11101 cctcgagcag cagttcgatg aatcctggct cgagtctgct tcgggcggac atccggacgc
    11161 atcgcccatc ttcgtgctcg gtcagccgcg taccggcacg acgctcgtcg agcgcatcat
    11221 atcgagtcac tcagaggtga cctccgccgg agaactgcag cagttcggcc tggggatacg
    11281 gcgtctttcg ggccatagag atccgaagcg tttttcgact gggctgtttg aggcctcgct
    11341 ggccgtggac ccgaagcagc tcgggggcgt gtacctggac accacacgcc gcatgcgcgg
    11401 tgacaccccc cgcttcgttg acaagctgcc ccagaactat ctctgcattc cgctgattct
    11461 ggccgcactg cccaacgcca agatcgtgca tctgacccgt gatcccatgg atgcctgctt
    11521 tgccagcttc aagcagcttt tcgcggacgc ctacctgcac tcttacgacc agcgggagat
    11581 ggcccgtcac cacgcccgat accgacggct gatggacgtc tggcgggaac ggttcccggg
    11641 aaggtttctg gacatctcct acgaggagac cgctgcagac ctcgagccca acgtgcgtcg
    11701 gcttttggcc ttcctggatc ttcccttcga ggaggcctgc gtcagcttcc acagccaggc
    11761 gagcgccgtc tccacggcaa gcgccgtcca ggtccgcgag cccgctcaca cccgctcggt
    11821 gggacgctgg aagcgatacg aacgtcagct ggagcccatg cgcgaagcgc ttgcaggagc
    11881 cggcgttgtc gggtgatcct accccttggg ggtattcagc caaggcggct gatcatcttt
    11941 actgtccgat gcgcatttga ccgtatgcgg ctctggtttg ctcagcggcc gcgaacgggc
    12001 gagcgaggag aaaaacatgc ccagacctgc acgacgcgcc ctgcgaaacc ccaacttcca
    12061 gctcaaaccc atcgccgctg cggtggccac cgcccttgcg tcctccgcgc cggccatggc
    12121 cgagcaggcg acggtcatag aggagatggt ggttaccgca accaagcgcg acgagtctgc
    12181 gcaggaggtt ccgctggcca tcaccgcgct ctccggcgag ttcaccagat cgtcgaacat
    12241 caacgacgtg aaggacctgg tggttttctc tcccggggtt tcgggtaaca gccaggacag
    12301 cttcatcgac gctatcagcg tgcgcggcat ccgtacccag gacttcggcg tcggcggcga
    12361 tccgtcggcc gcctttttca agaacgatct ttacgagggg cgcaacggct ctgcggtcac
    12421 cagcctcttc gacatggagc gggcggagat attgcgtggg ccccagggct tcctgttcgg
    12481 ccgcaactcc atcggcgggg cgttcagcgt gcatacggcc aaggcggaaa tcgccgatcg
    12541 cgcttcgggc tatgtggagg tcgatatcgg tgagcgggac cacctggttg tggagggggc
    12601 cgtcaacctg ccgatcaacg atcgcttcgc catgcggctt gcgggctaca gctccaacga
    12661 ggacggtttc gtcaagaacc ggttcaacga cgagaagctc atcgagcacg agaagtacgc
    12721 ccttcgctgg tccacggcgt tcgccacgga tcggctcagc ctggacacca tggtcgagta
    12781 cgaggagcgc gagcagtccg gatccgtata ccgggccata gatgagggcg acatctggga
    12841 gaccttcgaa gccgccatcg gcgaggtgga gcttcgaggc ggcccgtacg atgcggactc
    12901 ggaccagagc ggcggtgaca acgatgacgc cgatatcctc accattggcg tcaagttcga
    12961 gtacgagttc gactttgcga ccctgacctc gaacaccggg tacaaggatc acgagtacta
    13021 ctacaacgag gactacgacg gcacgcccct gaacctcaac aactatcggc aggatcagga
    13081 gggcgactac tttcagcagg agctgcggct gacctcgaag ggtgaaggcc cgctgtcatg
    13141 gtacgccggc gcgtcgtact acaaggagga gatcgacacc ctcttcgagt tcgccggtca
    13201 ggaagacttc ttctgccagt actacggtta ctactacaac tacggcatga cgttcaccgg
    13261 atgctctgat ctctatgcct attacggatc accgtttacg ccgtctgcgg acggggtgct
    13321 gcgggagacc ggccgcatcg tgggcgacta ctccggatgg gccgcttacg tggatctgac
    13381 ctacgagctc acacagcagt gggacttcag cgttgggctg cgctactcgg atgacgagaa
    13441 ggacttcacc atcaacgtcc cgacccccga cagcgacctc ggcgcctact gggcatacac
    13501 gttcgcaacc gacggagaca tcaccggcaa ggagagctgg gacgatctgc agacgcgatt
    13561 cgtggcgcgc ttccgcccca acgacgacgc gatgctgtat gcaagctaca ccgagggatt
    13621 caagtccggc ggtttcggca gcttcgcgct ggttgatgcc gacggggtgg gtgcgggcgg
    13681 tggagacacc gacctctccc agagcgatgg gtatcgtccg aatgccttcc agcccgaggt
    13741 cgccgactcc tacgagatcg gctacaaggg ctccctcgcc gaagggcgcg cgcagttgag
    13801 cctgaccggc ttttactacg agtacgagga cctgcaggtg gtcgtgttcg atgggggcgc
    13861 cgcggcggtg gagaacgtgg gcaacgtcga aggctggggc gtcgaaggaa cggcgaattt
    13921 cctgctcggc gactacgttg acgtttatct ggggctttcc tacctggata ccgaagccac
    13981 cggcctgcag agcatctgcg gtctggagga tgcggaaggc tgcgagggca gctcgttgtt
    14041 ctgggcgccg gagtggacgg gctcagtggt ggtcaacggc aacttcccgc tggaagccgg
    14101 gggcgccctc accggccgcg tggaagggtt ctgggaatcc gaacgcggcg gcggtttcga
    14161 ggggttgaag acaacggaga tcgattcctt cttcaatctg gcggtacgcg taggctacga
    14221 atcggagcac aactggtcgg ccaaccttta cgtcgagaac ctgacgaacg aggacacctt
    14281 cgacgggctc aacaacaacg gtggcgttct gccttcccac ttcttcggcc ccatgcggcc
    14341 gcggaccttc ggcgtgagct tcagctatgt ctgggattag gtgagccgct gctagcgcag
    14401 cggatcgcct ccctggtact cgtcgctgca gtccctcagc gcgcggatga acgtgtagaa
    14461 gagctcggat tggttgcgga tggcaagttt tcggtaggcg tttttccggt ggagcttcac
    14521 ggtctccacg gagatggcca acgactgggc gatggccttg ctcgtgtggc cgtgcaggat
    14581 cgagttgatg atctggcgct cgcgtgcagt cagaaccgat tcgccaaagc tctccagcgc
    14641 cgactccagc tgcgttcgca ggctgatctg ctcggtagac gggttggcgg gtagcagtcg
    14701 ccaatgatgc tgagtgagcg cggtgaccag aggcgcgaga tctgccaggc atctgagtgt
    14761 ggaggactcg aacggttggt tgccgtctgc gcgagccagg gaaagattcg caaagccgcc
    14821 gtccccgatg ggggtcagat agccgcactc gtcgaagatg ccgctgaacc ggtagtaggt
    14881 tcggtagtac tcgctggtct tgaacccggt gggggcgaga tgggaaagcg gaaagaatcc
    14941 gaatcggccc tcccgggctg cagcgacgta atacggatcg agcaggaagg gccccttgat
    15001 gaaggtgtcg agattgggtt cgtcccctgg tttctgagtt tcgcggtggg cgatgcgcgg
    15061 ggactcgtcg cggggataga cgatcatgca ggcctcgtcg atgttcacct gatccctgag
    15121 catcgcgacg acgctcgggg cgaattcatc ggtcccggct gccggcagga tctcagcgac
    15181 gtctctactg atgagtgcca gattcgacat gtttgcccac gatccgtcgc cgatcaccac
    15241 atcttatcgc cagccaggcc gactcgccac gcagccggag ggaggcgatg agcaggccgg
    15301 tcgtgctcat tccctgcgac gtgaagaggt atggtgagta cccgttccac tgcgtgggcg
    15361 agaagtacat caatgcggtc gcccacggtg cgggggcgat gcccctgctc atgccggcgt
    15421 ggggtcgggg cgcagagctg cgtgagctga cggatctgtt cgatctggac gcgatcctcg
    15481 accgggtcca tggggtattc ctgccgggat ctacgagcaa cgttcacccc caccgctacg
    15541 ggggcgcgga cctggatatg ctccatgacg agcagcgcga cgccgtcgtg tttcctctca
    15601 tcgagcgcgc cctgtcacgc ggtctgccgc tgctcgcggt atgcaggggt ttgcaggagc
    15661 tgaacgtggc tctcggcggc accctgcatc cgcggattca cgaggtgccc ggctatctgg
    15721 atcaccggga gaacgacgag ctgccgcggg atgcccagta cgggctggtt catgccgtcg
    15781 atgcggttca cggcggcatg cttgcagacc tggtcggcgc gaaacgtttt ctcgtcaatt
    15841 cgctgcatgc ccagggtatc gccgaactgg gggccgggct gcgcgtggag gcacgggccg
    15901 aggacggctt gattgaagca gtgtccatgg tcggcggctg ggtgctgggc gtgcagtggc
    15961 atccggagtg gcacttcacg gatgacaccg cgtctgccaa gctgttcgcc ggctttggcg
    16021 aagctttgcg ggcagcgtga ttgctgcccg gctggtcgac tgaggaggga gcggatgggc
    16081 aagccatcgg aaatgccgga cgaactggaa ctgctgctgt cggacatgaa cggcgtgccc
    16141 cgcggcaaga ccctgcgtgg aatcagctat tccgaagcgc acccgcccca cctggcggaa
    16201 gcgatctttt tccagaccat cacaggcggg tacgcggccg ccatggagac atacgacccc
    16261 aacgatcgcg atctgctttt gcgtccggat tggtcgacct acgtcccgac cccctggaag
    16321 ccggacacgg gccaggtgat ctgcgaaagc ctcgaccagg cgggtgaacg cctgccctac
    16381 gatccgcgta acgtgctgcg gagggtgctg gatgcttacg agtccatggg acttcgcccc
    16441 gtggtcgctc ccgaagtcga gttctatctt ctggaagctg tgtccagcca tgacctggtg
    16501 ctgacgccgg cgaccggaag gtcgggggct gccgagttcg gcggcgagtc cttcagcccc
    16561 gatgcgctgg agaagttcat gcccatcgtg gccgagattc agcaagcctg tcgccagacg
    16621 ggtctcgagc tctcggcgat ggtccacgag atgggacccg cccagatcga gctcaacgtg
    16681 gcccacggcg acgcgctctc ccgcgcggat cagctgtttc agctcaagcg gctgatcaag
    16741 ggctgcgcgc gcaagcacgg cgccctggca agcttcatgg ccaagccgct ggcagacctg
    16801 ccggggaacg ggctgcacct gcattgcagc atggaggacg agaccgggcg caatatcttt
    16861 gcgctcaatc gtcgccgggc gcccgccgtg ctccagcact tcattgcagg actgcaggcg
    16921 catctgcccc aggcgttcgc gttggttgcc cccaacgtga actcctacaa gcgcttcgtg
    16981 cgggatctgt cggcgcccat caacctggaa tggggttacg acaaccggac caccggcttc
    17041 cgcgtgccct tcagtgatgc ccgagcaggg cgcgtcgaga accgcgtggc aggtgcggac
    17101 gcgaaccctt acctcatcat tgctgcaacg ctcgcctgcg gactgctggg tatgcgtgct
    17161 gccatcaagc cgaccaagcc catcgccgac gatgcctacg gtctgaagcc ggcgcttccg
    17221 gaagatctgg gtgaagcgct gcggattctg gaacaaagcg aaggcctcat cgatctcctg
    17281 ggcgaggcgt tcgtggaggt cttcgtgtcg gtcaagcgtg gcgagcttga ccatttcggc
    17341 aagacaatca ctcagtggga agtcggctac ctgggcagca cgctgtgagc cgatctcggg
    17401 gcgggggaga tcccagggtc gggtttgacg agtcctggta ttaccacagc gcaaagacaa
    17461 tgcggaccta ccccggtctc gatggatcct gcgaagccga cgtctgcatc gtcggcggcg
    17521 gctacaccgg gctgtcggcc gccctggagc tcggccgcag aaattacgac gtggtggtgc
    17581 tggaagccca tcgggtcggc tcgggagcgt ccggacgcaa cggcggcgtg ctcggcatgg
    17641 gccagcgaaa agaccagctg gagctcgagt cctggctggg cgctgatgcg gcccggaagc
    17701 tgtggcggat cgcctgtgat gccaatgacc tggtgcgtga ccgcatcgcc gaattcggga
    17761 tcgactgtga cctgaaaagc ggcgagctca cggcagcgca ccgcgagcgt tacgagaaaa
    17821 cgttgtggtc gtacgccgag catctggagc gtgactacgg atactcggac cggagaatcg
    17881 tcagccgcga ggaagtggca aagatgctcg gcgcggacac cctgtacggc ggctacgtgg
    17941 atttgcgcgc gggccacgtg cacccgctga acctggccat cggcctcgcc cgtgcggcca
    18001 gcgatcacgg ggcaacgctt cacgagcaga cgccggccct gggctaccga caggaggggg
    18061 acagggtctt ggtgcgcacc gcatcaggcg acgtacgcgc ccgattcctg atcatcgcct
    18121 gcaacggtta tctgggacgt ttcgagaagg gcgccgagcg ctatcagatg cccatcaaca
    18181 acttcatcct ggccacggaa cccctggggg ccgagatggg gcgcgcgatc aatcgggacg
    18241 atctcgccgt ggtcgatacc cgcttcgtgg tgaactattt ccacaactcg ccggaccatc
    18301 ggctgatctt cggcgggggc gagaactact cgtcgcggtt tccgaaggac atcaaggcgt
    18361 tcgtgcggcg gcggatgctc cagatctatc ctcagctgga aagcgctcgg atcgacttcg
    18421 gatggggcgg cacgcttgcg atcaccatgc ggcgcatgcc gcagttcggg cgcaagggag
    18481 cgaacgtcta ctgggctcag ggatactcgg gccacgggat cgcgatggcc aacatgggcg
    18541 gtaagctggt tgcggacgcg attatgggcc aggccgagga attcgacacg tttgccgggc
    18601 tcaagcacct gcccttcccg ggagggcggt atctgcgctg gccggcgctg gtttcgggta
    18661 tgctttacta cgcttttctg gatcgtctat gagatgacgc gctccgagca cacgagatcc
    18721 gacctgctgg ggcaccgttc cgggatgacc tttgcgggcg agctttcctt ccttcgccgc
    18781 aagtacacca gggacatcga gggcgcagat ctggtggtga ccggtttgcc cttcgacatg
    18841 gcgacgactt tcaggccggg cgcgcgactg ggccctcagg ccatccgcgc ggccagcgtg
    18901 cagctggcgg gaattgccgg gcgggcgttt ccttacgggg aggatccgtt cgccacgcta
    18961 gctgttgccg actgtggtga ctgcttcatc gattccggtt acccccagca tggggctgcg
    19021 gccatcgaag cccatgcccg ggacatcatt gctcgaggct gcaaacttct gtcgttcggg
    19081 ggcgatcatt tcgtgaccta cccgctgctc cgggcgcacg ctgaaaagca tgggcccatg
    19141 tcgctgattc atttcgatgc tcacgtggat acgtgggagg acgacggcag ccgcatcgat
    19201 cacggctcca tgtttctgcg ggccctgcgg gagggtctga tcgtgcccga aaggtccgtg
    19261 cagatcggca tccgcagctt ttctcagcag gatcatggct tcgtggtctg cgacgcgccc
    19321 tgggttcatg aacacggtcc gcaggcggtg ctggagcgcg tcctgagcgt ggtcggccaa
    19381 cacccggcgt atctgacatt cgacatcgac tgtctcgatc ccgcctttgc ccccggcacc
    19441 ggcacgcccg tggcgggcgg cctctcgtcg gctcaggccc ttcacatcat tcgcgggctg
    19501 acgccggtga atctggtcgg gatggacgtg gtggaagtcg cgcctgcata cgatcacgcg
    19561 gagctgacgg ccattgccgc ggcgaccatc gcccacgacc tgatctgtca ggaagccttg
    19621 cgccgcggct gaggccgcgg cgcggactca aggtcgacgg gggctgccta cgcagccatc
    19681 ggagggacgg catgctccag tgccttggcg acttccggat gccggatgct gccacggtgc
    19741 acgttcaagc cgtttgcgaa atggcgatca cgggcaaaag cgtcttccca gccacggctt
    19801 gccagttcca tgacgaacgg aagggtcacc ttgctcaggg cgagggtgga cgttcgcgct
    19861 acgccccccg gcatgttggt cacgcagtaa tggacgaccc ccgcttcgac gtaggtcggc
    19921 tggtcgtggc tcgtggggcg gctggtctcg aaacatcccc cctggtcgat ggatatgtcc
    19981 acgatggccg ctccgttagc catggcttcc accatctcgc gggtaaccag cctgggcgct
    20041 gcggccccgg gcacgagaac ggcgcccacg acgaggtccg ccgtctcgac cgccttgccg
    20101 attgcgttcc cgtctgccat cagcacgcga gcccggccgt tgaagtggcg cctgagccag
    20161 tggagccttt gcgccgaacg gtccaggatg gtgacttccg cctgcaggcc cagggccatg
    20221 gtggccgcgt tggttccggc caccccgccg ccgagaatgg tgacgcgtcc gggtgccacg
    20281 ccggcggcac cgccgagcag cgttccgcgc ccgccgctgg ccatctcgag ggcgcgggcg
    20341 cctacctgaa tcgacatccg gccggccacc tcgctcatgg gcgtgagcag gggcagaccg
    20401 ccagcggggg acgtgacggt ttcgtaggcg atggccgttg cgccggagcg catcagcgcc
    20461 tcggcctggg gccggtcggc agccaggtgc aggtaggtaa agagcatctg accgtcgcag
    20521 agcatcgcgc attcctggag ctgcggttcc ttgaccttca cgatcagctc cgcggcgaag
    20581 gtctccttcg cgctgccgac ggcggcgccg gcgcgccgat agtcggcgtc agagaagccg
    20641 atggcggccg ctgcgtcgtg ctcgacgacc acttggtggc cggcggccgt cagcgctgcc
    20701 accgcagccg gcgtaagccc gacccggtat tcgtggatct tgatttccct tgggacccca
    20761 atgcgcatga cgtttctcca ggccttatca agtaacgaag cagaggagac atgctagctg
    20821 aacggcgagg ccgggttatt gcattcttgc tttaatttag ccctttggca caatatatac
    20881 gtcatatact ggaaaatata agcagaatat tgcatatgga tcgaattgat cgcgcgattc
    20941 tgcgggagct tcagaacgat gcccggctgc cgaacaccgc cctggcggaa cgggtcgggt
    21001 tgtcggactc cgcgtgtctg cgccgtgtga ggcgtctcga gcaatcgggc gtgatccggg
    21061 gctacgtggc gctggtcgac gagaccctgg ccggttaccc ggacagcgtg ttcgtgcaga
    21121 tcaccctggt cagtcagcag cggaaggatc tggaggcatt cgaggatgcg gtggccagag
    21181 ttccggaggt aatggagtgc tatctgatgt cgggcgcctc ggactacatc ctgcgggtcg
    21241 tggtcgcaga tgcccgggac tacgagcgta tccacagcca gcagctgacg cggctgccgg
    21301 gcgtggaccg ggtgcactcg aacttcgcgc tgcgcacggt catcgagaac acggagatac
    21361 cgattcgcac ctagccaagc gttaggggtt tccactagtg gtccagagta agatcgcact
    21421 agcagaatcg gtgagggtgg cagattgatg ctggattcgc cggggggata agtcggccgc
    21481 ggcgtgcttc gacgacgggg aggcggatga gtcgggatgg aaatttctga ccaatctcaa
    21541 ttggcgctga tcgccctgtg catctacttc gtcgtcgtcg tggggctggg cctcttctat
    21601 tcccgaaaaa gccaatcgac cgaagatttc atcctggccg ggcacagcct cacgacgccc
    21661 ttcgtcatgg gctccgtggt tgcgacctgg atgggcggag cggtgatact cggtggggcc
    21721 aaggaagcct tcgtcggagg ctttcaggcc atcgtctggg atccgtggtc gcccatgctg
    21781 accctgattc tgtgtggttt cttctttgtc agcgtgttcc ggcgttcccg cttcaccact
    21841 tccatcgact tctacaatgc ccggtacaac cgtgggatcg ggcttgcggc gctgagtatc
    21901 agcatggtgg cgtatatctc ctggatatcg gcgcagctgc tctctctcgg tgtgatcatc
    21961 aaggtcgtaa ccggaatgga cgcggtcatg gcgactctgg ctggcagcat gatcgtgctc
    22021 gtcgtttcgc tgtccggcgg gctgtgggcg ctgtcccgat ccgacatgct cgccttcatc
    22081 atcctttccg tggtgctgat cctggtgctg cccctggcgc tggatgcggt cggtggtccc
    22141 gctgctttca tcgagaacgc gggcaccagg gatggatcgc cgcccttcag cttgttctac
    22201 accgaggcgc caaccgcggc tggcgaaccg acgggcttct acggctacct gggcatgctc
    22261 ggcgtcttct acatgctggc cgcctggttc tcggttgcca tcggcgatct cggcggctcg
    22321 gtgctcaccg cccgggctct ggcggcaaag gacgaagtct cggcaacgcg gggctttctt
    22381 ttcggcgggg tcatctacct gctgcttggg atgattccgg tgatcgtggg catgtgcgtc
    22441 ttcatcctga gtcccgacat cgcagaagcc gatctggacg acgtgcttcc gatcttcgtg
    22501 cagcaatacc tgccggattg gatcacggtg ctgtttttcg ttgccgtggc gtccgccatc
    22561 gtcagcacgg ccggggatac catcctgact ggcggcgccc tgctgggcta cacggcgctc
    22621 ggtgccgtca agccggagag ctccgatcgg accagactgc gcgcaacgcg gaccgcgatg
    22681 gttctgttca cggccatcgc gctcgtgttc ggcctcgcca tgggcgacct ctaccggctg
    22741 ctggtgtttg cgggcgcggt ggcgttcccg gtcacctcgg cggccttcgt atgcggcgtg
    22801 ctgtggaagc gggccaacgt cccgggcgcc tgggcgtcga tcgctgctgg caccgtcagc
    22861 tgggtgatct tcgtcgtgct ccttctgccc gaggtcgact gggaagtctg ggatgccctc
    22921 tacatcggcg cggtccccgc tttcctttgc agcgcgattg ccatcgtggt tgtgtccctc
    22981 gccacgcgca ggacccaccc gcccaatccg gtacgcgacg tggatggaaa cgacatttcc
    23041 ggtgggccgc tgttcagatg gcgatgacga ggctgcgcaa acgcgcgcgg ctcggcagtc
    23101 cccccaccgc gaccgcctac gaaagctgcc gctgcgtatc cttatctcct cgtcaggttg
    23161 ttggccggtt cctctgatgc cggtgggtca cgccagccga tgccactaag tggaaccccg
    23221 aatcaccaaa tcccagtcag acccgcatca tcagaggcta gaccaatacg tggagatgcc
    23281 atgggaatcc cgatccgagt tctgatcgtt ctgctgtctg ctcttgccat tgccggttgc
    23341 cagactgccg cgccggaggg tgaacggtct ggctacgagt acgatggcag aaacctctat
    23401 ctgggttact gcgcgagctg ccacgggttg agcggcgcag gcgacggtcc ggtggctgca
    23461 ggcatggcgt tcaagatgga agatcttcga acgctcgagg cgcgcaacgg aacctttccc
    23521 gccgacgggt tgagagaagt cattgacggg cgcaccttgc ggtctgtgca cggcaccaac
    23581 gacatgccgg tgtgggggtg gcagttcctc cttgcagagg actcggagga gcacgtagcg
    23641 gcgcgcatcg acgctctagt cgagtatctg cgatccatac agcacggcga ctcagatccc
    23701 gcggcgccct gaccagcggc caggcgagtc gaattggcgc gccaaccgct ccgccaagtc
    23761 accagagccg atatcccagg cgacgtgccg ctcgagcacc cagtccgcca gccccagctg
    23821 ctccatcgcc tttggaaaag cccggccacc gtggtcgctc acgaagagca cgcgagcgct
    23881 gccttccggg ttgaaaacgg tgaaaggagg cggatcgtcc ggtccgatga gaggctgctc
    23941 ggtcatgagg aatggttttc ctactcctga ttcttcgtga gcagatggca agggcgcgaa
    24001 gcaggtcgag ttggctcacc gttttctcca gtcgtccttc gccgcctcgg cggctttcgc
    24061 tgccatcgct cgggggtcgt actgccagtc gccgtcgtag cggggcgcgt acgtctcgta
    24121 tgccttcatc atctcgtcgt agcgatgctg gatccaaacc acgttgtagg ggtgcgacac
    24181 cacgtagtac tcgccgttta gaatctgcgg ccaggcaatg gacgtgtact cttccacgtc
    24241 catggggatg tcgcccgcca ttgcggtcat ttcagactgc acgaaccccg ggcagatgaa
    24301 ggaaacgtca atgaagtcgg gcgtttcctc gcgcagcgca tccgtcatgg aatgcaggcc
    24361 atgcttggcg ctctcataag cgaacatgcc gggaacgatg ttgaagaacg cgttctcgga
    24421 cccgacgttg tagatagccg cgggcgtgcc ccgttcgatg aagcgcttgc cgaagacgga
    24481 tgctccgttc cagacgccca tatagttcac gtccaacagc tggcgcacca gcttcggatc
    24541 ggcatcaatc accgtggatg gcgggatcag aattccagca ttgttcatga tgacgtcggc
    24601 cccgccgaat ttggcttcag caaaatcggc cagcgcttcc acttgcgtca agtcagagac
    24661 gtcgcacacg aagcctgcag cttcatagcc aagctgctgc agggcggcga ccgactcatc
    24721 caacttttgc tggcgcctgc cggcaatgat caccttggcg ccagccgcgc caaacctttt
    24781 ggcgaacgag aaaccgatgc cggtggcgcc cccggtgatt acggcgactt tgccttcgaa
    24841 gttgttcaag ccgttctccc tcgagtcgtt gcgagtacat acgctcgagt atacagactt
    24901 gactggtgcc gcttagtgcg gggcagccct cggactcggc tcaacggtgg taccctaact
    24961 cacttgcctc acctcgaagt ctggagatcg caagctgaac atcgaactca ccgtgaacga
    25021 agcgcgcgtg ctcggttgcc tgatggaaaa atcggtcatc acgccggatc aataccccct
    25081 gacgttgaac gcgctgacca atgcgtgcaa ccagaaatcc agtcgcgatc ccgtgctctc
    25141 tctcgagccg ggcgtcgttc agcgaacggc tcgtgatctc gaagccaagc acttgatcgc
    25201 cagcgacgag aatttcaaga gccgggtgga gaaattcagc cagcgtttct gcaacacgcc
    25261 gtttgccgat gtgcagttgg acagcgcgga attcgccgtg atgtgcctgc ttttgctgcg
    25321 gggtccccag acgccgggcg aattgcggtc ccgcagcgga cgcttgcatg acttcgccga
    25381 caaccaggcg gtggtggaaa cgctccaagg cctgctggaa cgtgaaggtg agccgctggt
    25441 ggcacggctg ccgcgcaaac cgaatcggca ggaccacgag tatgcgcacc tgctgtccgg
    25501 caccatcgag tcgatgccgg ttgcggaagc ggcggcccac gtgcgcgccc ccggccgtac
    25561 ggccgagctc gagggtcggg tggccgcgtt ggagcaggag gtgcgcgagc tgcgcgaagc
    25621 gctgcaacaa atgcgcgcag agcgcgagga agagaagtca tgacagactg gctaaaagca
    25681 ggcaacaacg cggtgatcac cggcggcgcc agtggcatcg gcctggcggc agccacccac
    25741 tatctttcgg ccggcatgaa cgtgctgatc gccgatctcg acgaggacgc tctgagcaca
    25801 gcggcagcgt ccctggctga cagtggcagc ggcaccctgc acactcaggt ctgcgacgtc
    25861 tccgactttg cgcaggtgga gacgctgcag gccacagccg cggcaaagct cggcaacgtg
    25921 cactgcctga tgaacaacgc aggtgcgggg ttcccgccca gcgcgccctg ggagaacctg
    25981 gacgtctgga agaagcaggt ggagatcaac ctctggggca tcattcacgg ctgccaggcc
    26041 ttcgtccctg ccatgctgga agccggcgag cggggagcga tcgtcaatac cggctcgaag
    26101 cagggcatca ccaacccgcc gggcggctac gcctacaacc tgtccaaggc gggcgtgaag
    26161 aactacaccg agagcctggc ccacgcgctc cggcaggtgg aaggctgcca gctctccgcc
    26221 cacctgctgg tgcccggctt cacctacacc ggcatgatct cccgcttcat tcccgagaag
    26281 ccggccggcg cctggacgcc cgacgaggtg gtggcatttc tgattcgatc gctcgatcga
    26341 ggcgacttct acgtgctctg cccggacaac gacacgccgc gggacctcga cgagaagcgc
    26401 atccagtgga acacggacga cctgatcagg aaccgatccg ccctgtcccg ctgggatccg
    26461 aagtacaagg acgcctatga ggccttcgtc tccgactgac aatgcctgga gttccgtggc
    26521 tggcggttga gcgcgcctcg cggcgccgct ccgatcgcct accagggcca gctcatcgtc
    26581 agctccggct acggatttac cggcaagatg tcgggcaacg cgctgctcgt tttcgaggtg
    26641 gcaacttacg gggcgggcgg aacccaagaa cctcaaccgt tgtgcacccg tcacgttttc
    26701 tcaaaccaac cacgatcctt ccacaaaacg ctcgcatcag ggtcgaaagt gtcgaccgcc
    26761 tcacgggaag acacgtcccc ttttccgata ctcgagccgt tctgacgcta tgcgccgcag
    26821 atggcaccca ccgatccgcc gcgcagatgt tggctttaag agcggagagg caaagtggga
    26881 cgaaacggag agggaagggc ccccagggca acgcgggggt ttcaggcagg ctgcctgagt
    26941 gcgctacttt ttctgggcgc atgcaccggc agcagcgaaa agattttcga gcaaagtgaa
    27001 gagctgagtc ttcgcggcag cgttggggac ggccccatcg tcggcgcgca gatcgtggtg
    27061 gaggacgccg aaggccaaat ggtgttcacc gccgagagcg atgccgccgc caactatcag
    27121 atcgatgttc ccgacggcac cgatctgcct gtgaccattc gcgtgagcgg cggcaccgat
    27181 ctggtcaccg gccgcggcgc ggattttgaa cttctggctg tggcgttcac gtctggcgat
    27241 ctcaccgtca acgcctcacc ctacacgacc ctggcagtga agaccgccca gtgcatgggt
    27301 ggactcagcg aggccaatct cgctaccgcc tggcaataca tcgatgccaa catgaacctc
    27361 ggctgggatg gggagatcat cccgaatccc atgaacgagc cgattgccga tgacaacatc
    27421 gccaccgtgc tggtgggtaa cgaagccctc ggtgaggtga tccgccgcac gagcgccgcg
    27481 ctggcgccga ccacggaagc gctctccacg gacgaagtga tccagatgct tgcctgcgac
    27541 attgctgacg gccagctgga cggcgtcgga ccagatgtgt ctccccgggt cgcgctcacg
    27601 ctgctcggca cggcaaccgc ggtcagggtg gaagtcatca gcggtgccct gttcgtcgat
    27661 ggacaggacg ccaccccgct cctggacagc gcgctgcaga ccgtgctgcc gggcagtctc
    27721 cgctccgtca ctgagctggc gcccaatcag cagctcatcg accagaccct gagcggcctg
    27781 gcggtgcttg ggacgttcga ctttgccgaa gccacgactc ttgcgatcgc cctcgaaggt
    27841 gcaaccccgg ataccgcccg cggccagctg gcgccggctc tcaccagctc cgtgaccacc
    27901 aatctccagg acctgggcag tcagttggct ctggcggata cgacaaccat agaggagctg
    27961 ttgctgcgcc aagccgacag cgccggtgcg ccggttccag tcgtttcgtt tgatgcggag
    28021 ccatcgtctg tcgaggcggg aaccagcacc cgcctgagct gggcggccag cgacgccgac
    28081 gagtgcctgg cctcgggtgg atggtcaggc tcgcaggcca ccagtggcac ttttcagacc
    28141 ccgctgctgg agcagagcac ggaatatgtc ctgagctgcg tgggcctggg gggcacgggc
    28201 accgggcgag tgtttgtaga agtgactcaa cccgcaccgg tcccggcaac ggacgcgggc
    28261 tccgacccgg acccggcttc agacccggac ccggtctccg atccgatctc agatccggac
    28321 ccgatctcgg accctgatcc gatctcggac ccggacccgg tctcagatcc ggaaccggaa
    28381 ccggaaccgg aacccgcacc ggctccggcg cctcctcctg cgccgcccgt cgtctccatc
    28441 agcgccgatc gtacgac
//
LOCUS       DBNHLM010000210         7556 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_688102, whole genome shotgun sequence.
ACCESSION   DBNHLM010000210 DBNHLM010000000
VERSION     DBNHLM010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7556)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7556)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7556
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_688102"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="AC16QF_09720"
     CDS             <1..529
                     /locus_tag="AC16QF_09720"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448875.1"
                     /translation="HLCCNTAGGGLAKRTLTKEGPHPLEEFQRVIDLNLISSFNISRL
                     IAAKMAGNEPNEDGERGCIINTASIAAFEGQIGQVAYSAAKAGIAGMTFVMARDLGSL
                     GIRANAIAPSLFDTGLTSGIPDEFALALTRDAAFPKRMGKPVHYAIQAIAIYENVMMN
                     GGTVRVDAGQRFGPK"
     gene            547..1008
                     /locus_tag="AC16QF_09725"
     CDS             547..1008
                     /locus_tag="AC16QF_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtoQ family protein"
                     /protein_id="HIR6448876.1"
                     /translation="MQNGQWQVYLSGEIHTDWRDQIASGAAARGLPVAFTSAVTVHEH
                     SDDCGVRILGAEPDDFWKDHKGAKVNAIRTRTLLEQADVVVVRFGDKYRQWNAAFDAG
                     FAAALGKPLITLRDDSLTHPMKEVDAAALAVARDPAQVVQILDYVIAGRLS"
     gene            complement(1028..2236)
                     /gene="nhaA"
                     /locus_tag="AC16QF_09730"
     CDS             complement(1028..2236)
                     /gene="nhaA"
                     /locus_tag="AC16QF_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003184695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HIR6448877.1"
                     /translation="MVLDSLRQFFRLEAAGGVLLVAAAALALLISNSPLTGLYEAFLD
                     VPVVIQVGALEIAKPLVLWINDGLMAVFFFLIGLEVKREVLEGELSSPEQIVLPAAAA
                     VGGFVVPALIYWFFNQSDAIGDDGWAIPAATDIAFALGILALLGNRVPVALKVFLVSL
                     AIFDDLAAIVVIAVFYTSELSVLSLSLAIVGIAMLSILKWRRVQSVGPYVLVGLFIWV
                     CVLKSGVHATLAGVATALFIPMHNQTGDYGGFPLKHLEHVLHPWVAYMILPLFAFVNA
                     GVSFAGMSPDMLVEGVPLGVALGLFVGKQVGVFLTCALLIALGFARMPAGSNWASLYG
                     VSVLTGIGFTMSLFIGSLAFEAAGYGYHAGVRAGVLIGSALSAVLGYAVLWLSLREPA
                     SSREDSANAS"
     gene            complement(3411..3497)
                     /locus_tag="AC16QF_09735"
     tRNA            complement(3411..3497)
                     /locus_tag="AC16QF_09735"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3461..3463),aa:Leu,seq:caa)
     gene            3619..5076
                     /locus_tag="AC16QF_09740"
     CDS             3619..5076
                     /locus_tag="AC16QF_09740"
                     /inference="COORDINATES: protein motif:HMM:NF036848.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HIR6448878.1"
                     /translation="MSNTPILVGVAQVEQRIEDVREGEEPLAMMIDATRLAARDAGCP
                     GLLSEANSVRVIRGMWPYANPARAVGEAIGNPGAETALTPFGGNSVQSTLNHSAIDIR
                     DGVHDVIIITGAECGRSQAKAGRAGVDLNWSEVPGTPDRYIGEDMEMRCPAERGLGMG
                     RPIQMYPMMENHLRYRRGDTIEEQLIRISELWASFSAVAAENPHAWMREAKTAEQLRT
                     ISVENRPVSFPYPKLMNSNNNVDQGAALILCSTEKAKALGIPEEKWVYPWAGTDAHDT
                     YFVSNRDSMSRSPAIRLAGRRCLELTGLTPLDLAFVDVYSCFPVAVQVAAEEIGLDTT
                     RPLTITGGLTFAGGPLNNYVMHSIATMVEKLRGARGEKGLITANGGYLTKHAFGVYGT
                     EPPATPFQHEDLQPAVDATATRKVVTEWTGEATVESYTVMYKGDELAVGHLSCLTPDG
                     VRTWANCEDPDTLLAMTREEFCGRPVRLADRRAHF"
     gene            5051..5521
                     /locus_tag="AC16QF_09745"
     CDS             5051..5521
                     /locus_tag="AC16QF_09745"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR6448879.1"
                     /translation="MPTGGHISEATPTDANGQPGATRRHAGLLRRVGAMCYDALLVVA
                     LWLVTLFPIVALSNSAVTGATVRSLLFIELFVFFAYFWVSRGQTLGMLAWRLRLVPAA
                     PGEPVTLRQALLRFIGALAAFATLGLGYLWMYIDPERRTWPDMLSGTRVIHEPR"
     gene            complement(5526..6602)
                     /gene="lptG"
                     /locus_tag="AC16QF_09750"
     CDS             complement(5526..6602)
                     /gene="lptG"
                     /locus_tag="AC16QF_09750"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HIR6448880.1"
                     /translation="MLAVMDRHIGRTVLATTMMVLIAFLGLMTIFALIDELGGSRESY
                     GFGEALTYVALTTPRRIYEMLPYMVFLGALIGLGSLASNSEIVVLRAAGVSVWRVFLS
                     VAWPAFIVLLLGFLLGEFVAPRGEELAEAHKTRSRQDSEVIVLRGGYWYREANLYMNV
                     DGLDGQGGLIGLRQFWLDAEENLVRSVSASRAEYLPGADPHWMLFDVVETAFQDESTS
                     VQRSESARWDGNVDPRLLGVRVLVEPRKLSVTDLSYQIDYMTREGLNSGSYQLAYWGK
                     LLQPLSVLGLALLALCFVLGPLREVGMGVRLSVGVIAGLSFKYLQDLFAPMSYVYDLP
                     PPLAVALPIIVCWAIGLVGLRRVA"
     gene            complement(6606..>7556)
                     /gene="lptF"
                     /locus_tag="AC16QF_09755"
     CDS             complement(6606..>7556)
                     /gene="lptF"
                     /locus_tag="AC16QF_09755"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HIR6448881.1"
                     /translation="GKYAAQGLLTIMWLRLPEFLQQLLPFAYYVALLLTVSRWHADRE
                     MTIVQNSGVGGGRLLRWLGIPAVLLAALVGLLSMVITPANNAILEDFFQEQRANEEFT
                     AVSPGIFRTFARGNRVTYTESVSDDQRELQGVFMAERRPDRQDVTVWAESGRQVVDPD
                     TGSRFLVLNNGTRYEGRIGDRNYRVVEFGALGQRLALQPAAQRSTEVESLPTRTLMGA
                     GRPDEIAELHWRYSLPLLVLIATGIAVGLGRVKPRQGRFAKIVPGILVFVGYYVALVA
                     VRNALEDGVWPPALGLWPVHLAFLGVSVALLTAASRPAKI"
BASE COUNT         1586 a         2403 c         2308 g         1259 t
ORIGIN      
        1 gcacttgtgc tgcaacaccg cgggcggcgg gctggcgaaa agaacgctga ccaaggaagg
       61 gccccacccg ctcgaggagt ttcagcgggt aatcgatctg aatctgatct cgtccttcaa
      121 catctcacgt ctcatcgcgg cgaagatggc gggcaacgag ccgaatgagg atggcgagcg
      181 cggttgcatc atcaacaccg catccatcgc cgcgttcgaa ggccagatcg gtcaggtagc
      241 atactctgca gcgaaggcgg gcattgcggg catgactttc gtcatggcga gggacctcgg
      301 cagcctgggc attcgagcga acgccatcgc gccgagtctt ttcgatacgg ggctgacttc
      361 gggcatcccc gacgagttcg cgctggcctt gaccagggat gcggcgtttc cgaagaggat
      421 gggcaagccc gtacactacg cgatccaggc aatcgccatc tacgagaatg tcatgatgaa
      481 tggcggcacg gtgagagtgg atgccggcca gcgattcgga ccgaaatagt tcgaagggag
      541 agtttcgtgc agaacgggca gtggcaagta tatctatccg gtgagatcca taccgactgg
      601 cgcgatcaga tcgccagcgg agcggcggca agaggccttc ccgtagcttt caccagcgcc
      661 gtcaccgtgc acgagcacag cgacgactgc ggcgtgagga tcctgggtgc agagcccgac
      721 gatttctgga aggaccacaa aggtgccaag gtgaacgcca tccggacccg gacgctgctc
      781 gagcaggccg acgtggtggt ggtcagattc ggcgacaagt atcgacagtg gaacgccgca
      841 ttcgacgccg gttttgccgc agcgctgggc aaacctctca tcactctccg cgacgacagc
      901 cttacccatc ccatgaagga agtcgatgca gcggcgctcg cagtggcgag agaccccgcg
      961 caggtggtgc agatcctcga ctacgtgatt gccggccgat tgagctgagc cgccccgccc
     1021 agaggggtta cgaggcattc gccgaatcct ctctcgagga cgcgggttca cgaaggctga
     1081 gccagagcac ggcgtatccg agcacggcgg acagcgcgga tcctatcagc acgccggctc
     1141 gcacgccggc atggtagcca tacccggccg cctcgaatgc aagcgagcct atgaacagac
     1201 tcatggtgaa acctatcccc gtgagcaccg acacgccgta tagcgaagcc cagttcgatc
     1261 ctgcaggcat gcgtgcgaaa cccagcgcaa tcagaagcgc gcaggtcaga aataccccaa
     1321 cctgcttgcc gacgaagagc ccaagagcga ccccaagcgg aaccccctcc accaacatgt
     1381 ccggcgacat tccggcaaac gaaacgcccg catttacgaa ggcaaacagc ggcagaatca
     1441 tgtaggcaac ccaaggatgg agcacgtgct cgagatgctt cagcgggaac cctccataat
     1501 ctccggtctg gttgtgcatg gggatgaaca gcgcggtcgc gacgcccgca agcgtggcat
     1561 gcacgccgct tttcaggaca cagacccaga tgaacagacc aaccagcacg tacggcccga
     1621 cactctggac ccgccgccac ttgaggattg agagcatggc aatgccgacg attgcgagcg
     1681 aaagcgacaa tacggacagc tcgctggtat agaagacggc tatcaccacg atggcggcca
     1741 gatcgtcgaa gattgccagc gataccagga ataccttcag cgcgacggga acccggttcc
     1801 cgagcagggc gaggatgccc agcgcgaacg caatgtcggt cgccgccggt atggcccaac
     1861 catcgtcgcc aatcgcgtcg ctttgattga agaaccagta gatcagcgcc ggcaccacga
     1921 acccgccgac tgccgccgca gccggcagca caatctgctc tggagacgaa agctctccct
     1981 ccagaacttc tctcttgacc tccagcccga tcaggaagaa gaaaacggcc atgaggccat
     2041 cgttgatcca caacacgagg ggtttcgcga tctcgagagc acccacctga atcacgaccg
     2101 gcacgtcgag aaaggcttcg tagagcccgg tcagcgggga gtttgatatg agcagcgcca
     2161 gcgcagccgc cgcaaccagc agaacaccgc cagcggcttc caacctgaag aactgtcgta
     2221 atgaatcaag caccatgagg cattccgttt gcgccccttc tacgggccgc cggttccgac
     2281 aatgtcgaca ccagaatcga cgccagagtt tcgctccaat cctgacggct caatcgcaat
     2341 ctctcgtgcg gaggcgacgt gcttgcagat gccggcgcgc gccactccaa gaacgagcat
     2401 tacggcctcc ctttgcagta gccttgggtc acacgatggc tcggccttga gcaaacttcg
     2461 accggataat tatggacgat gggtcgccgc attcggaatt tttctcaatc gctggctagg
     2521 catggctttt ggccaacgtt agtcaggtac gtatacgttc aattgcgtcg tctgatcgac
     2581 ttcgagatct gtcgggtcga gtccggatca gaggatcctt acacttggcc aaagctcgaa
     2641 ggatatgagg tacgcgcagt cgacgagcag cagtttcacg acaatctctg cgaggaacta
     2701 agtgatgcca gatttgacag cgagttccag cgcggagatc tatgcattgc cgcgctctgc
     2761 gagggtacga tcgtagcgta cgactttact tctaagcacc cgtctcgcgt gaaggagggt
     2821 ttggagttta ctttcccgga cgaattttcc tacgggatgg tcacaacaac cgcaccggct
     2881 catcgaggcc gaaagatcgc gcgagagttg tggagagtca gtcgccaaat tcggttacaa
     2941 cgcgagggcc gcgatgtgct tcgcatctgg tacgtcaatg cgaccaattt ggagtcgcga
     3001 gcagcgaacg tcaatactgg cacaaaggag aactttcagg ggtatgccgg ctatctgcga
     3061 ctgttcggac gctggcacgt gttcgcttct ccgagatgta gaaggtttgg ggccgggttt
     3121 cggcagtccg atagctacac tcccggcagt cccaccactt aacgtctgaa gtccaaagag
     3181 gcaggtggca cagcggtcgc ccatacgctt cgacattttt agaataatga aaataaagct
     3241 tgagggccaa agaatgcgac gtgctggaag ccgagatatc gcgcctcaga tcaggggtgc
     3301 gttcacgaga gccgcgctgc aactcgaaga ggaaggcagg ccgatggggg agttagaggc
     3361 ttcgttcagc attcaggtga acttcgtgga gcccgaacct gcatttttac tggtgcctgg
     3421 ggtgggaatc gaacccacac ttggttgccc aaaccagatt ttgagtctgg cgcgtctacc
     3481 agtttcgcca cccaggcagc gatcgcggat tgttgcacag gccgagaaag cgcggcaatg
     3541 gcacctttgc gacagcgcag cttcctttcg gtatcatccc cgccccttaa aagattcagt
     3601 caggatttct tcgagaacat gagcaatacc cccattctgg ttggcgtggc ccaggtcgag
     3661 cagcggatcg aggacgtgcg cgaaggcgag gagccgctgg cgatgatgat cgacgccacg
     3721 cgactggcgg cgcgggacgc tggctgcccc ggcctgctca gcgaggcgaa ctccgtgcgg
     3781 gtgatcagag gcatgtggcc ttacgccaat cccgcaaggg ccgtgggtga agccatcggc
     3841 aatccgggcg cggagaccgc gctcaccccg ttcggcggca acagcgtgca gtcgacgctg
     3901 aaccatagcg cgatcgacat tcgcgatggc gtccacgacg tcatcatcat caccggagcc
     3961 gagtgcggcc gcagtcaggc taaggcgggg cgagcgggcg tcgacctgaa ctggagcgaa
     4021 gttcccggca cgcccgaccg gtacattggc gaggacatgg aaatgcgctg ccccgccgag
     4081 cgcggtctcg gcatgggtcg ccccatacag atgtacccga tgatggaaaa ccatctgcgc
     4141 tatcgccggg gcgataccat cgaggaacag ttgatccgta tctccgagct gtgggcgtcg
     4201 ttcagcgccg tcgccgcgga gaatccccac gcgtggatgc gcgaggctaa aaccgcggag
     4261 cagcttcgaa cgatctccgt ggagaaccgg cccgtctcct ttccctaccc caagctcatg
     4321 aactccaaca acaacgtgga tcagggcgcg gccctcattc tctgctcgac cgagaaagca
     4381 aaagcgctcg gcatccccga ggagaagtgg gtgtacccct gggcgggcac cgacgcccac
     4441 gatacctact tcgtgtcgaa ccgggacagc atgagccgct cgcccgccat tcggctcgcc
     4501 ggccgacgct gcctcgagct gaccggcctg acgccattgg atctggcctt cgttgacgtc
     4561 tacagctgct tcccggtggc cgtgcaggtc gccgccgaag agatcggcct ggacacgaca
     4621 cggccgctca ccatcaccgg gggtctgacc ttcgccggag ggcccctgaa caactacgtc
     4681 atgcactcca tcgccacgat ggtggagaaa ctgcgcggcg cccggggtga gaaaggcctg
     4741 atcaccgcca acggtggcta tctcaccaag catgctttcg gcgtgtacgg taccgagcca
     4801 cctgcaacgc cgttccagca cgaggacctg cagccggccg tcgatgccac ggccacccgg
     4861 aaagtcgtca cggaatggac gggcgaggcc acggtggaga gctacacggt catgtacaag
     4921 ggtgacgagc tcgccgtcgg gcacctgagc tgcctgaccc cggacggtgt gaggacctgg
     4981 gctaactgtg aggacccgga cacgctcctg gccatgaccc gggaggagtt ctgcggcagg
     5041 cccgttcgac ttgccgaccg gcgggcacat ttctgaggct acgcctacgg acgccaacgg
     5101 gcaaccgggc gccacgcgcc gccatgccgg gctgctgcgg cgcgtcggcg ccatgtgcta
     5161 cgacgcgctg ctcgtcgtcg cgctctggct cgtcacgctg ttcccaatcg ttgcgttgag
     5221 caactccgcc gtcaccggcg ccacggtgcg atccctcctg ttcatcgaac tcttcgtttt
     5281 ttttgcctac ttctgggtga gccggggcca gacgctcggc atgctcgcct ggcgactgcg
     5341 cttggtaccg gccgcacccg gggaacccgt caccctgcga caggcactgc tgcgcttcat
     5401 cggcgccctc gccgcgttcg ccaccctggg tctcggatac ctgtggatgt acatcgaccc
     5461 cgagcgccga acgtggccag acatgctgtc cggcacacgg gtgatccacg aaccccgctg
     5521 atcgctcagg ccacgcggcg cagccccacc aggccgatgg cccagcagac gataatgggc
     5581 agagccaccg ctagcggagg cggcaggtcg tagacgtagc tcatgggcgc gaagaggtcc
     5641 tggagatact tgaaggacaa acccgcgatg acaccgacgc tcagccgcac gcccatgcct
     5701 acctcgcgca aagggcccag gacgaagcag agcgcgagca gagcaaggcc gagcacggag
     5761 agcggctgca gcagcttgcc ccagtaggcc agctgatagg aacccgagtt gagcccctcc
     5821 ctggtcatgt agtcgatctg atagctcagg tcggtgaccg acagcttgcg gggctcgacg
     5881 agcaccctga cccccagcag acgcggatcg acgttgccat cccagcgagc cgattcgctg
     5941 cgttgcacgg acgtggattc atcctgaaac gcggtttcca ccacatcgaa gagcatccag
     6001 tgcggatcgg cgccgggcag gtactctgcc cgggacgcgg acaccgagcg caccagattc
     6061 tcctctgcgt ccaaccagaa ctggcgcaga ccgatgagcc cgccctgccc gtccaggccg
     6121 tccacgttca tgtaaaggtt cgcttcccga taccagtagc caccgcgcag aacgatcact
     6181 tcggagtcct gtctgctgcg cgtcttgtgc gcctcggcga gttcctcccc ccggggggca
     6241 acgaactcac cgagcagaaa cccgagcagc agcacgatga acgccggcca cgccacgctc
     6301 aggaataccc gccatacaga gacgccggcc gcgcgcagca cgacgatctc cgaattgctt
     6361 gccaggctgc caagcccgat gagggcaccg agaaacacca tgtacgggag catctcgtag
     6421 atgcgccgag gcgtggtcag cgccacgtac gtaagcgctt cgccaaaacc gtaggattcg
     6481 cggctgccgc caagctcatc gatgagcgcg aagatcgtca tcagtcccag gaaggcaatc
     6541 agcaccatca tggtcgtcgc gagaacggtc cggccgatgt gtctgtccat taccgccagc
     6601 atggatcaga tcttcgctgg ccgcgacgcg gcggtgagca gagcaacgga gacgcccagg
     6661 aacgccaggt gaacgggcca gagccccagc gctggtggcc agactccatc ctccagggca
     6721 ttgcggaccg cgaccaacgc gacgtaatac cccacgaaga ccagaatgcc cgggacgatc
     6781 ttcgcaaagc gcccctgccg cggtttcacg cgccccagcc ccacggcgat tcccgtggcg
     6841 atgagcacca gcagcggcag ggagtagcgc caatgcagtt ctgcaatctc atccggccgg
     6901 cctgccccca tcagcgtccg ggtcggaaga gattccacct ccgtgctgcg ttgggccgcc
     6961 ggctgcaggg cgagccgctg cccgagcgcg ccgaactcca cgacccgata gttcctgtct
     7021 ccgatgcgcc cctcgtaacg ggtgccgttg ttgagcacga gaaaccggct gccggtatcc
     7081 ggatccacga cctgccgacc ggactcggcc catacggtga cgtcctggcg gtctgggcgg
     7141 cgctctgcca tgaacacgcc ctgcagctca cgctggtcgt cgctgacgga ttccgtatac
     7201 gtcacccggt tcccgcgggc gaaggttcga aaaattccgg gcgagaccgc cgtgaactcc
     7261 tcgttggcgc gctgctcctg aaagaaatcc tccaggatcg cgttgttggc gggcgtgatg
     7321 accatgctga gcaaaccgac caaggccgcc agcaataccg ccggaatgcc cagccagcgc
     7381 aggagccgcc caccgcccac gccgctgttc tggacgatgg tcatctcacg gtcggcgtgc
     7441 caccgggaca ccgtgagaag cagcgcgacg tagtacgcga agggcagaag ctgctggagg
     7501 aactccggaa gcctgagcca catgatggtg agaagaccct gtgcggcgta cttccc
//
LOCUS       DBNHLM010000211         9354 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1527477, whole genome shotgun sequence.
ACCESSION   DBNHLM010000211 DBNHLM010000000
VERSION     DBNHLM010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9354)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9354)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9354
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1527477"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1544
                     /locus_tag="AC16QF_09760"
     CDS             <1..1544
                     /locus_tag="AC16QF_09760"
                     /inference="COORDINATES: protein motif:HMM:NF039580.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR6448882.1"
                     /translation="KLHGLERVMEIAETHTPIYTPSHRSHVDYLLLSYLLHKNGLMIP
                     HIAAGDNMNLPFVGHLLRRGGAFFMRRSFRDDPVYTAVFSEYLYQVYRRGHCVEFFPE
                     GGRTRTGRLLPARMGLLKMTLEHHRRGVPRPLAIVPVYLGYEKLIEGGSYLDELRGSE
                     KQRESIGDIFRSIKLIRQNFGQVNVNFAKPIDLNEWSNAHPDVPENELPARLGQEILE
                     RINESAAINPINLVALVTLATPKLAIDEMLLIDQIGCYLELLRAEAPQHDYTVTDQSP
                     AEVVGYVERLGMLTREEAEFGDILCHDPFAAVLMTWYRNNVTHTLALPSLIACMVINR
                     RRPMGKAALTRMVDTVFPYLAAELHMVGDHAAIDRWLKHLVRANLLELHPAGGYCAPS
                     VRSQSRYRLHLLSRLIMQTLERLYIVVALIGQPGITVRTRSDLETQSQQVARKMSRIH
                     GLNAPEFFDSRLFNGFIDQLIANDIVTESTDGSLTYRPIISNVIKAAESIIDAEFRYA
                     VLRGA"
     gene            complement(1520..2242)
                     /locus_tag="AC16QF_09765"
     CDS             complement(1520..2242)
                     /locus_tag="AC16QF_09765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016340.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6448883.1"
                     /translation="MRLYVSARQRDEIRRYQDLFDIVVTSGPPVDEDGPFLYWNGERL
                     QLFSGHDGKPAAVCDEEIERRATFSSELARACGVTRSRQPSVLDALAGWGVDGMSLAA
                     LGSSLVMVEQNDAMWALLDSFLAHHPGQRPELIHDDAWAVLDGSRSFDVVSLDPMYAR
                     RRKGALPGKSMQLLALVCGPDQRSAADWIDRARPCAAGRVVLKRRLRDPLVGRPDWQI
                     KGHSVRYDVYRGRGDQAPLSTA"
     gene            complement(2286..2969)
                     /gene="tsaB"
                     /locus_tag="AC16QF_09770"
     CDS             complement(2286..2969)
                     /gene="tsaB"
                     /locus_tag="AC16QF_09770"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR6448884.1"
                     /translation="MTTILALETSGSLCSVALICETGSFENTRNVERMHNQVLLGMLD
                     DLFEQAAVAPAGVDVVAFGAGPGSFTGVRIAAAVAQGIGVGIGGKVVPVSSSLARAAV
                     AFREHPDHTAVITLIRSRRDAYYLAGYTRSSGKIAISFADVLVTAWPDGLWDGQRLAV
                     GDLPDWWSSDRPETLNFGGEALVTAPVIAELGLAAAHAGGAFDPALGLPVYLKGDSPW
                     KPVDSGGSG"
     gene            2963..4195
                     /gene="moeA"
                     /locus_tag="AC16QF_09775"
     CDS             2963..4195
                     /gene="moeA"
                     /locus_tag="AC16QF_09775"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF007960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR6448885.1"
                     /translation="MSSKYSIGPVDLNEAVERITAGIDPIEGTEVTGLANATGRIAAE
                     DVSALVSLPPFPSSAMDGYALRSEDIQGNPPYELKLVGQSLAGRPFDGSPGVGEAVRI
                     FTGAAMPQGADTVVIQEDCTATGEGVRINTRPNAGDNVRCEGHDVGRGEIIVRRGQPL
                     TPFSVGWLAASGHESIAVSARPRVAVFSTGDELQEPGSALGPGQIYESNRHMLRGLLQ
                     QLPVEVTDLGRVADDPDETIDALQRAATQADAMITTGGVSVGDADFVADAVESLGSLE
                     VWRMNIKPGKPFAFGRVGSCLFFGLPGNPVSAAITYLMIARPALVRLCGAEAGEAPSA
                     LARLTDPISHKPGREEFQRGRMRVSGGETTVSVTGDQSSNRLATFDKANCLIRIPKQA
                     GSLAPGATVEVLPLFGLI"
     gene            complement(4325..4771)
                     /locus_tag="AC16QF_09780"
     CDS             complement(4325..4771)
                     /locus_tag="AC16QF_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448886.1"
                     /translation="MPRAKKAAAKRKTAAKKAPAKRKTAAKKAPAKRKTAAKKAPAKR
                     KAAAKKKAPAKRKTAAKKAPAKRKAAAKKKAPAKRKTAAKKAPAKRKAAAKKKAPAKR
                     KTAAKKAPAKRKTAAKKAPAKRKTAAKKAPAKRKTAAKKTAAKRKR"
     gene            complement(5023..5673)
                     /gene="adk"
                     /locus_tag="AC16QF_09785"
     CDS             complement(5023..5673)
                     /gene="adk"
                     /locus_tag="AC16QF_09785"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010797616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - AMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR6448887.1"
                     /translation="MKVILIGPPGAGKGTQAQFICEAFGIPQISTGDMLRSAVQQGSP
                     LGQRVKAVMDSGALVSDDIIIELVKERIADEDCAEGFLFDGFPRTIPQAEALSEAGVS
                     IDFVVEITVPDEEIVKRMSGRRVHPQSGRVYHVDFNPPKVAGVDDQTGEELIQREDDR
                     EETVRERLAVYHQQTHPLVNFYEQKAQSNELSYLSVDGQGDVNEIQQRVLAGLKIN"
     gene            complement(5670..7688)
                     /locus_tag="AC16QF_09790"
     CDS             complement(5670..7688)
                     /locus_tag="AC16QF_09790"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:NF024724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR6448888.1"
                     /translation="MVKVGDSVPRHADGRIDILSWSENVCEDRSALDPARIRQAAELV
                     RQIEDAPDRYLETGVALGGLVADLMMDTPTVCAALLYRPVRAGALELGRVGDALDAET
                     AELLGSVVRMASTSILEMSNAPLQTIESRDQIENVRSMLVSMIDDARVAVLKLAERVV
                     ALRAAKDGPDERRQRIAREAHLIFAPLANRLGIWRLKWELEDLALRYLEPEVYMDIAR
                     RLDGRREEREAQVASVVSSLEEKLRAAGIDAAVSGRAKHIFSIYRKMRAKKVGFEEVY
                     DVRAVRIIVPDIAQCYASLGIVHGLWRHIPSEFDDYIAVPKENGYRSIHTAVMWPGGG
                     TLEVQIRTHEMHQESELGVCAHWDYKGGDREAMPYSEKMNWLRQVVDWQDTAGAWNLT
                     RGIESELAERVREERIFVYTPQGHVLDLTAGATPVDFAYRVHTEVGHSCCGARVDGRT
                     VSLNTPLETGQRVEIVTAKYQSPNRAWLDRKLGYVRTARAREQIQDWFRERPDYENRA
                     FGITMIADMSDRLCVAKPDSRTLGSVATELGLDDEEGLLRALAVGDCQIADVVECLYP
                     AEQSARQMSLIPPSDDGVAETYAVAIEATDREGLLRDISVLLTQSHLALVGNTGRVDP
                     DTKRAHLLFELKLGGLAQLAVIVDQLRQIPDVIDVRRVIDQNDREKGA"
     gene            complement(7688..9016)
                     /locus_tag="AC16QF_09795"
     CDS             complement(7688..9016)
                     /locus_tag="AC16QF_09795"
                     /inference="COORDINATES: protein motif:HMM:NF017743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR6448889.1"
                     /translation="MSRRRIREVEVAVTALGEDGLGEGQFEGRYVRIRNALPGETVTA
                     RVLKRRAGALLAEAVEVRDRAPLRRAAPCTVFPRCGGCSLQHLEHGAQLRHKEKVLLD
                     ALAANGVVPQSVRRPVSGPRLHYRHKARLGVRCVGDNLLVGFRESFSSRVVRMADCRI
                     LAEPLARCLPDLQSTLSSLRERHRVPQVELVAGDDSQAVIVRHLDPLGPESQVRLEEM
                     SRRLRMRVYLQAGGYDSLMLLGAPGKRPKLTYSIMEFGVTYRFDVADFVQVNPAINRA
                     LASSVAVALDPRPGITIADLFCGIGNFSLVLARHGAKVKGFEASAGAIERARENADLN
                     GLEHRCEFDVADLYDSGYAIPAGTQRLLLDPPRSGAGPHLARWLSGTELERVVYVSCN
                     PMTFASDAAVFEKQGFALIDVGVFDMFPQTAHVETLGVFVPSYSRTGASR"
     gene            complement(9020..>9354)
                     /gene="recO"
                     /locus_tag="AC16QF_09800"
     CDS             complement(9020..>9354)
                     /gene="recO"
                     /locus_tag="AC16QF_09800"
                     /inference="COORDINATES: protein motif:HMM:NF014607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR6448890.1"
                     /translation="SSQDAELAVTLRSFEKLLLQELGYGLDFRHDASSGEPIAQDVIY
                     ELDVQRGFFPGQPAEGFEGRLLLEIAEENFESAAVRQAAKRIFRLALGELLGSKPLTS
                     RRLLLRGG"
BASE COUNT         1795 a         3076 c         2721 g         1762 t
ORIGIN      
        1 tgaagctgca cgggctagag cgggtgatgg agatcgccga gacacatact cccatctaca
       61 ccccatctca ccgaagtcat gtggactatc ttctgctttc atacctcctg cacaagaacg
      121 gcctgatgat tccgcacatc gcagccggcg ataacatgaa ccttcctttc gtgggtcatc
      181 tgctgcggcg cggaggagcc ttcttcatgc ggcgcagctt ccgtgacgat cccgtataca
      241 cggccgtttt ctcggagtac ctctatcagg tttaccggcg cggtcactgc gtggaatttt
      301 ttcccgaggg tggacgaacg cgaaccggac gattgctgcc cgctcgcatg ggactgctca
      361 agatgaccct ggaacaccat cgacgtggcg taccccggcc cctggccatc gtgcccgtct
      421 acctcggcta cgagaagctg atcgagggcg gcagttatct ggacgagtta cgcggctcgg
      481 aaaagcagcg cgagtccatc ggggacatat tccgcagcat caagctgatc cggcagaact
      541 tcgggcaggt gaacgtcaac ttcgcgaaac ccatcgacct caacgagtgg agcaacgctc
      601 acccggacgt tcccgaaaac gagcttcccg caaggctcgg ccaggagatt ctggagagga
      661 tcaacgagag cgccgcgatc aaccccatca acctcgtggc gctggtcacg ctcgctacgc
      721 ccaagctcgc catcgacgag atgctcctga tcgaccagat cggctgttac ctcgaactgc
      781 tccgggcgga ggcgccccag cacgactaca ccgtcaccga ccagagcccg gccgaggtgg
      841 tcggctacgt cgagcgcctg ggcatgctga cccgggagga ggccgaattc ggggacatcc
      901 tctgtcacga cccgtttgcg gccgtgctca tgacctggta ccggaacaac gtcacccaca
      961 ccctggcgct tccttcgctg attgcctgca tggtcatcaa tcggcgccgg ccaatgggca
     1021 aagcggcgct gacacgcatg gtggacacgg tattcccata tcttgccgcc gagctccaca
     1081 tggtcggaga ccatgcggcc atcgatcgtt ggctgaagca cctggttcgc gcaaatctgc
     1141 tggagcttca tccggccggc ggctattgcg ccccatccgt caggagccag tcccgctacc
     1201 gactgcacct gctttcgcga ctgatcatgc agacccttga acggctctac atcgtggtag
     1261 cactcatcgg tcagccgggc atcacggtcc gtacgcgcag cgacctggaa acccagagcc
     1321 agcaggtggc acgcaaaatg tcccgcatcc acgggctgaa cgcgccggaa ttttttgatt
     1381 cgaggttgtt caacggcttc atcgaccagc tgatcgcgaa cgacatcgtt accgagtcga
     1441 cggatggaag cctcacctac cgcccaatca tcagcaacgt gatcaaggca gcggaaagca
     1501 tcatcgatgc cgagttccgc tatgccgtgc tcaggggcgc ctgatccccc cgacctcgat
     1561 agacgtcgta tcgcacgctg tgacccttga tctgccagtc gggccgaccg acgagcggat
     1621 cgcgaagacg acgcttcaac accacacgcc ccgccgcgca aggtcgtgcg cgatcgatcc
     1681 agtcggcggc cgatcgctgg tcgggtccgc aaaccagcgc cagcagctgc atcgactttc
     1741 ccggcagcgc gcctttgcgg cgacgggcgt acatcggatc cagagacacg acgtcgaacg
     1801 atcggctgcc gtcaagaacg gcccacgcgt cgtcgtgaat cagttcgggc cgttgccccg
     1861 ggtggtgcgc cagaaagctg tccagaagcg cccacatggc atcgttctgc tcgaccatca
     1921 ccagcgagct gccgagagca gcaagggaca tcccatcgac gccccagcca gccagggcgt
     1981 cgagcacgct tggttgccgg gagcgcgtca ccccgcatgc ccgtgccagc tcgctgctga
     2041 aggtcgcgcg cctttcgatc tcttcgtcgc agacggccgc cggcttgcca tcatgcccgc
     2101 tgaacaattg cagacgttcg ccgttccagt acagaaaagg accgtcctcg tcgacgggtg
     2161 gtccactcgt taccacgata tcgaacagat cctggtaccg gcggatctcg tctcgctgtc
     2221 gggcggatac atacagacgc atcgggtagc gcctcagttg acgtctggct gacggctgaa
     2281 ggccgtcacc cggacccgcc gctatcgacg ggcttccagg gcgaatcgcc cttcaagtag
     2341 acgggcaacc caagggccgg atcgaacgct ccgcccgcat gagctgccgc gaggcccagt
     2401 tcggcgatca ccggcgccgt caccagcgcc tcgccaccga aattcagcgt ttcgggcctg
     2461 tccgaactcc accagtcggg cagatcccca acggccagcc gctggccatc ccacaagcca
     2521 tctggccacg ccgtcaccag cacgtccgca aacgaaattg cgatctttcc agacgatcgg
     2581 gtgtagccgg caaggtaata ggcatcccgc cgggaccgga tcagagtgat taccgccgta
     2641 tgatcaggat gctctcgaaa cgcaacggct gctcgggcaa gcgacgagga aacgggaacg
     2701 accttgccgc ctatcccgac accgatgccc tgggccacag ccgcggcgat tctcacacca
     2761 gtgaacgaac ccggacccgc gccgaacgca accacgtcga caccggccgg cgccaccgcg
     2821 gcctgctcga acaggtcatc gagcattccc agcagcacct gattgtgcat gcgctcgacg
     2881 ttgcgtgtat tctcgaaaga gcccgtctcg cagatcaacg cgaccgagca gagcgatccc
     2941 gaagtttcca gagccagtat cgttgtcatc caaatatagt atcggacctg tcgacctgaa
     3001 cgaagcggtc gagcgcatca ctgccggcat cgatcccatc gaaggtacgg aggtcaccgg
     3061 cctcgccaac gccaccggcc ggattgctgc cgaggacgtc agcgcgctgg tcagcctgcc
     3121 cccctttccc agctccgcga tggacggcta cgccctgcgc agcgaggata tccaaggcaa
     3181 cccgccgtac gagctcaaac tggtcggtca gagcctggcc ggtcgcccgt tcgacggcag
     3241 cccgggggtc ggagaggccg tgcgcatctt taccggcgcg gcaatgccgc agggtgcaga
     3301 cacggtggtc attcaggagg actgcaccgc aacaggcgaa ggagtccgca tcaacacccg
     3361 gccgaacgcc ggtgacaacg tgaggtgcga ggggcacgac gtcgggcggg gcgaaatcat
     3421 cgttcgacgc ggccagccgt tgaccccctt ttccgttggc tggctcgccg cttcgggaca
     3481 cgaatccata gcggtctccg cgcgtccgcg cgttgccgtt ttttcgaccg gcgacgagct
     3541 gcaggaaccg ggctccgctc tagggcccgg tcagatctat gaatccaacc gacacatgct
     3601 tcgcggtctt cttcagcaat tacccgtcga ggtcaccgat ctcggtcgcg ttgccgacga
     3661 tcctgatgaa acgatcgatg ccctccagcg ggccgccacc caggcagacg cgatgatcac
     3721 taccggcggc gtttcagtgg gcgatgcgga tttcgttgct gatgccgtcg agtccctggg
     3781 tagcctcgag gtctggcgaa tgaacatcaa acccggcaag ccgttcgctt tcggacgcgt
     3841 tggaagctgt ctgttcttcg gcctgccagg caatcccgta tcagccgcga ttacgtatct
     3901 gatgatcgcg cgtccagccc tcgtcagact ttgcggtgcc gaggcaggcg aggcgccgag
     3961 cgctctggct cgcctcaccg atcccatcag ccacaaacca ggacgcgaag aatttcaaag
     4021 aggacgaatg cgggtatcgg gaggggaaac aacggtttcc gtcacaggcg atcagagctc
     4081 aaaccggctg gcgaccttcg ataaggccaa ttgcctgatc cggattccaa agcaagcagg
     4141 atcgctcgcg cccggcgcca ccgtggaggt gctgcccctc ttcggcctga tatgaggcga
     4201 acccacctgc ggcagacact cagcaacgcg tcctaaagcg ggagaaaaga ccgatttgcg
     4261 gagacgggca aactgccgtc aggaccaggt cccgacggct ttgcccaaaa aattcaccga
     4321 gcgtttagcg cttccgcttc gccgccgtct tcttggcagc cgtcttacgc ttggcgggtg
     4381 ccttcttggc agccgtcttg cgcttggcag gtgccttctt ggcagccgtc ttgcgcttgg
     4441 cgggtgcctt cttggcagcc gtcttgcgct tggcaggagc cttcttcttg gcagcggcct
     4501 tacgcttggc gggtgctttc ttggctgcag tcttgcgctt ggcgggtgcc ttcttcttgg
     4561 cggcggcctt acgcttggca ggagccttct tggctgcggt cttgcgctta gcgggtgcct
     4621 tcttcttggc ggcggcctta cgcttggcag gagccttctt ggctgcggtc ttacgcttgg
     4681 cgggtgcttt cttggctgca gtcttgcgct tggcgggcgc tttcttcgct gcagtcttac
     4741 gcttggcagc tgccttcttg gctctcggca tgaagcatct cctcagcttg tttacttcag
     4801 tgtgtacccg ttggcttcct tgtcaatgac ccaatgctga tccgcctgct gaaagctact
     4861 gctgatcgaa ggcaaccaat ggccattcat ctttccctgc gctctcgcca actcgctcaa
     4921 acgcgttata tggttttgcg cgttaccggg ctttcgtcgc ctctgcaaaa ataaacttgt
     4981 gcaaaggcgg gagaatgata tttatcagaa gattgattgt cactagttga ttttcagccc
     5041 tgcgagaact ctttgctgaa tttcgttgac gtcaccctga ccatccacac tcagataact
     5101 cagctcgttg ctctgcgctt tctgctcata gaaatttacg agcggatgcg tttgctgatg
     5161 ataaacagcg agcctttcgc gcacggtttc ctcacggtcg tcctcacgct gaatgagctc
     5221 ctctcccgtc tggtcatcca ccccggcgac ttttggcggg ttgaaatcga catggtagac
     5281 gcggccggat tgcggatgaa cacgccgtcc gctcatccgc ttcacgattt cctcgtccgg
     5341 cacggtaatc tcgacaacga aatcgatgga aacgcccgct tcgctcagcg cctctgcctg
     5401 gggaatcgtt cgtggaaacc cgtcaaagag aaatccttcc gcacagtcct cgtcggcgat
     5461 ccgctctttg acgagctcga tgatgatgtc gtcggaaacg agcgcgcccg agtccatcac
     5521 cgccttgacg cgctgtccga ggggcgagcc ctgctgaacg gccgagcgga gcatgtcccc
     5581 tgtcgatatc tgcggtatgc cgaacgcctc gcagatgaac tgtgcctgcg tgccctttcc
     5641 cgctcccggt ggcccaatta agatgacttt cacgcgccct tctccctgtc gttctgatca
     5701 atcaccctgc gcacgtcgat gacgtcgggt atctgtcgta gctgatcaac gatcaccgcc
     5761 aattgcgcga gccccccgag cttcaattcg aacaggaggt gcgcacgttt ggtatccgga
     5821 tccacccgcc ctgtattccc caccagagcg agatggctct gggtgagaag aacgctgatg
     5881 tcgcgcaaca acccttcgcg atcggtcgct tcgatcgcaa cggcgtaagt ctccgcaaca
     5941 ccatcatcgg acgggggaat aaggctcatc tggcgcgccg actgttcggc cgggtaaagg
     6001 cattctacga catccgcgat ctgacaatcg cccacggcca gcgcgcgcag caagccttcc
     6061 tcatcatcga gtccaagctc ggtcgcaacc gatcccagcg tccgggaatc gggtttcgcc
     6121 acgcacaacc gatcgctcat gtcggcaatc atcgtgatgc caaaggcacg attctcatag
     6181 tcggggcgct cgcgaaacca gtcctgaatc tgttcccggg cgcgtgcggt gcgaacataa
     6241 ccaagcttgc gatcaagcca cgcccgattc ggcgactggt atttggcggt aacgatctct
     6301 acccgctgtc ctgtctctaa cggcgtgttg agactgaccg tgcgcccgtc cacccgcgcg
     6361 ccgcaacagc tgtgaccaac ctcggtatgc acgcggtacg caaaatcgac gggggttgcg
     6421 ccggcagtga ggtcgaggac gtggccctga ggcgtgtaga cgaaaatgcg ctcttcacga
     6481 acccgctcgg caagctcgct ttcgatgccg cgagtaaggt tccaggcacc ggcggtatcc
     6541 tgccagtcca cgacctgacg cagccagttc atcttttcgg aatacggcat cgcttcccga
     6601 tccccgcctt tgtaatccca atgggcgcag acgccgagct ccgattcctg atgcatttcg
     6661 tgggtacgga tctgtacttc gagggttccg ccacccggcc acatcaccgc cgtgtgaatg
     6721 gaccgatagc cgttttcttt gggaacggca atgtagtcgt cgaactcgct gggaatgtga
     6781 cgccacagac cgtgcacgat gccaaggctt gcatagcact gcgcgatgtc gggcacgatg
     6841 atccgcaccg cacgcacgtc gtagacctct tcgaatccga cctttttcgc acgcatcttc
     6901 cgatagatgc tgaatatgtg cttcgccctg ccgctgaccg cggcgtcgat accggcggca
     6961 cgcagctttt cctccagcga agagaccacc gacgcaacct gggcttcccg ctcttcacga
     7021 cggccgtcca gccggcgtgc gatgtccatg tagacttccg gctcgaggta gcgcaaggca
     7081 agatcctcga gttcccactt gagtcgccag atacccaggc gattggccag cggagcgaat
     7141 atgaggtgcg cttcccgcgc aatccgctga cgtctctcgt cgggcccgtc ctttgcggct
     7201 cgcagcgcga caaccctctc cgccagcttg agcacggcaa cccgagcgtc gtcgatcatc
     7261 gagacgagca tgctgcgtac attctcgatc tggtcgcgcg actcgatggt ctggagcggc
     7321 gcgttcgaca tttcgaggat gctcgtgctc gccatcctga cgaccgaccc aaggagttcg
     7381 gcagtttccg catccagagc atccccaacc cggccgagct ccagcgcgcc ggcacgtaca
     7441 gggcgataaa ggagagcggc gcagacggtg ggggtatcca tcatcagatc cgctaccagg
     7501 ccacccaggg cgacgccggt ttccagatac cggtcgggcg cgtcctcgat ttgccgaacg
     7561 agctccgccg cctgtcggat tcgagccggg tcgagcgcag agcggtcttc gcagacgttc
     7621 tcagaccagc tcaggatatc gatgcggcca tcggcgtgcc ggggaacgct gtcgcctacc
     7681 ttgaccatca gcgagatgcc ccggtgcgcg agtaagaggg gacgaaaacc ccgagggttt
     7741 ccacgtgcgc cgtctgcggg aacatatcaa agacgcccac gtcgatcagc gcgaaacctt
     7801 gcttctcgaa gacggcggca tcgctggcaa acgtcatggg gttacaggaa acgtagacca
     7861 cccgctcgag ctccgtgccg ctcagccaac gggcgaggtg gggaccggca ccggaacgcg
     7921 gtgggtcgag aagaagccgt tgggtgccgg cggggatcgc atagcccgaa tcatacagat
     7981 cggctacgtc aaactcacat cggtgctcga gaccattgag gtccgcgttt tcccgggccc
     8041 gctcgatagc gccggcagac gcctcaaagc ccttgacctt tgcgccatgc cgggccagca
     8101 ccagagaaaa attgcctatg ccgcagaaga gatcggcgat ggtgattccc ggccgcgggt
     8161 ccaaggcgac cgctacggac gacgcgagcg cgcgattgat cgcagggttc acctgcacga
     8221 aatccgccac gtcaaaacgg taggtgacgc cgaactccat gatggaatag gtcagcttcg
     8281 gccgcttccc tggcgctccg agcaacatga gggagtcgta tccaccggcc tgcagataga
     8341 cccgcatccg aagccttcgc gacatctcct ccagcctgac ctgggattcc ggaccgagcg
     8401 gatccagatg acgaacgatc accgcctgcg agtcatctcc agcgaccagc tctacctgag
     8461 gcacgcggtg tctttcgcgc aggctcgaga gggttgactg aaggtccggg agacatcgcg
     8521 cgagcggctc ggcgaggatc ctgcaatccg ccattcgaac cacgcggctg ctgaaacttt
     8581 cgcgaaatcc caccagcagg ttgtcgccga cgcagcggac cccgagccgg gctttgtgac
     8641 ggtagtgaag ccgaggaccg gagaccggcc ttctcacgct ctggggaacc accccattgg
     8701 ctgccagcgc atcgagcagg accttctcct tgtgccgcag ctgagcacca tgttcgaggt
     8761 gttgcagact gcagccaccg cagcgcggaa agaccgtgca gggggccgcg cgccgaagcg
     8821 gcgcccgatc gcgcacctcg acggcctcag caagcagcgc gccggcgcgt cgcttgagca
     8881 ctcgcgcggt gaccgtctcg ccaggcaggg cattgcggat cctcacgtac cgaccctcga
     8941 actgcccttc acccagccca tcctcgccaa gagcagttac cgccacctcc acttccctga
     9001 tgcgacgacg gctcaatgct cacccccccc gcagcagcag acggcgactg gtgagcggtt
     9061 tcgaacccag aagctccccc agcgcgagcc ggaatatcct cttcgcagcc tgcctgaccg
     9121 ccgccgactc aaagttctct tcggctatct cgagaagaag ccggccctca aacccctcgg
     9181 cgggctgccc gggaaagaag ccgcgctgca cgtcgagctc gtagatcacg tcctgcgcga
     9241 tgggctcgcc ggaagaggcg tcgtggcgaa aatctagccc gtagcccagc tcctggagaa
     9301 gaagcttctc gaaactgcgc agcgtgaccg cgagctcggc gtcctgtgat gaca
//
LOCUS       DBNHLM010000212        11604 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_688460, whole genome shotgun sequence.
ACCESSION   DBNHLM010000212 DBNHLM010000000
VERSION     DBNHLM010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11604
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_688460"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="AC16QF_09805"
     CDS             <1..342
                     /locus_tag="AC16QF_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448891.1"
                     /translation="GPTGPVGNMSLITITEHQVGYVIAMLERMKKRRLVAVSAREDAY
                     RNYNQAMQEAVKNTVWMTGGCTSWYIDKTGIPNLYPWEPMRYLKSMRNPDFSEYREET
                     GQGKEAALAAA"
     gene            complement(347..1858)
                     /locus_tag="AC16QF_09810"
     CDS             complement(347..1858)
                     /locus_tag="AC16QF_09810"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0032787 - monocarboxylic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6448892.1"
                     /translation="MNGLGDWIRCNADWAGGRPALRLGETAYTYAELDAAVDRMAFLL
                     RDGLGIRRGDVVAFLGLNSEQMIVLLFACARNAASLLPLNNRLAGPEHEWILRHSGAR
                     FLFVEDEFAAQANAIDVPGLERVAMGAPPEPHWHEFQRMLGDAREGGFEDVGTLDDRV
                     LLIYTSGTTGQPKGVAHDQRALFYNALNAIHAQEMRASDHVLTVLPLFHAGGLNIQTT
                     PAFYIGAGVSLMRRFDPGEALRAIETLKPTMFLAVPAVITAMVDHPAWSETDLSSLRM
                     VGTGSSTVPDPLLDAWHDRGVPATQIYGLTESAPTAICLPIAETRRKRGAAGKPVIHC
                     EARIVDDGGNILGPGERGEIVLKGPNLFREYLNDPETTAAAFSDGWFHTGDIGHMDAE
                     GFFYVDDRKKDVVISGGENVYPAELENVLAEMPELAEYAVVGRPDERWGEVPVACVVL
                     RDGADLGEDELIARFAGRLARYKHPREVRFFQSLPRNVMGKVLKFRLREEVAP"
     gene            complement(1863..3053)
                     /locus_tag="AC16QF_09815"
     CDS             complement(1863..3053)
                     /locus_tag="AC16QF_09815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6448893.1"
                     /translation="MTKTEELIGIARELGRDRFLPRRRTYDEEARFPDENYEDLREAG
                     FLGLAIDRRYGGLGADFGTYASISVELGRWCGATALTFNMHTSTMLWSGQLADDLPMS
                     DAERSAHESRRAEIYAGVIEEGHIFAQPFSEPNSAAAAGKAAFDTSAVKVDGGWLVNG
                     RKHFASLSGSAHFYGVLCTDLTDGREPDVRNTLLLAVPASASGFSVAGDWDVLGMRGT
                     VSRSLVLEDVFVPDSGQLLPQGCYYQAALGWPHMFMTLCPTYLGIAEAAFDFTCQYLR
                     GEVEGGPPAGGARDNPVKQVGVAEMRIKLEQARALFEKAIGEAAYRPSKDERLRAYAA
                     QYTVMEFANDICRQAIRVCGGRSLFKSLRLEQLYRDSRCGSLMLPWTPDICIERLGRE
                     SLES"
     gene            complement(3053..3628)
                     /locus_tag="AC16QF_09820"
     CDS             complement(3053..3628)
                     /locus_tag="AC16QF_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6448894.1"
                     /translation="MSVPGFKEAGSGEPVVFLHGIGGNRHAFDAQLAEPGRVRSLVLA
                     QTMARFGKPGSDWQQQFLDARLKPLEDGRAPADFARELITGMFGDPSRAEAIERAVAT
                     MSPLPADVYRQVLTCLVVFDQRGTLPEIDAPTLCLAAELDQTAPPRTMEKMATEIPAG
                     RYDCLQDAGHLAYVEGPAAFTRAVNEFLGQL"
     gene            complement(3625..4671)
                     /locus_tag="AC16QF_09825"
     CDS             complement(3625..4671)
                     /locus_tag="AC16QF_09825"
                     /inference="COORDINATES: protein motif:HMM:NF053113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-dihydroxypyridine 5,6-dioxygenase NicX
                     family protein"
                     /protein_id="HIR6448895.1"
                     /translation="MLQERIEGKWIDCFARVFERCAVAENEEVAILSESQSRPANAQL
                     AELALLRLGARPYHLVIPTPPQKAPVPVRSTGATDVIGHSRSVVAALSSAGFVVDCTV
                     EGMLHAPELPEILKGGARVLMISNEHPEALERLEPDEALETRVKAGIRKLRGASQMKV
                     TSAAGTDLTVSLEGSVAGGGWGYVARPGIMSHWPGGLCLCFPAAGSVSGVLVLDRGDV
                     NLTFKRYLENPVALSIDADYVTEIDGAGVDAELMRSYFAAWGDQEAYAASHLGWGMNN
                     AARWDAMTMYDRNDFNGTELRAFGGNFLYSTGANEVAGRHTLGHFDLPLRNCSVHLDG
                     EAVVVDGALQRELA"
     gene            4802..5047
                     /locus_tag="AC16QF_09830"
     CDS             4802..5047
                     /locus_tag="AC16QF_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448896.1"
                     /translation="MSLIRYPVVQYAYVVDDIEAAGYPAVTTLTSAARAAYMDTRPIL
                     GCFVELYEDNAPLRETFAHWKAVHDAWNGVSDPIRDA"
     gene            5040..5633
                     /locus_tag="AC16QF_09835"
     CDS             5040..5633
                     /locus_tag="AC16QF_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6448897.1"
                     /translation="MPDPEQPGELPFRPADAILRPESFLPAADGTEVCAFLNPYDARG
                     AASAHSAGEAISMAAGRIGPGVRSAIHVHPALTQITYVTAGTLTARMRDPGESEPSRL
                     EVPTGAAVLTEAGTLLQLCNETDRTVEVLYVSTPAYVLETAADGAIRYEDALILGADW
                     DAPAVAHWNVETATQARSRATARRAEAAGRIAGRTRR"
     gene            complement(5652..6272)
                     /locus_tag="AC16QF_09840"
     CDS             complement(5652..6272)
                     /locus_tag="AC16QF_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_259446992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6228..6230),aa:Sec)
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HIR6448898.1"
                     /translation="MTDQLKLEIFSDYIUPWCYLSTGRIEKLKSSFDLEVRWVAFPLH
                     PETPEEGITIEALFAGREIDLDAMYVRMKALMDEEGLPYGRRTHTFNSRLAQELGKWA
                     DSRPGGEAIHDALFRAYFVDGLNIGDAGVLVDIAKSAGLDPGGAREVIDQRTFSNAVD
                     EDWKRSRRYGITGVPTFVAGGYGVVGAQPYETLEHLVQRALTESRA"
     gene            6395..9616
                     /locus_tag="AC16QF_09845"
     CDS             6395..9616
                     /locus_tag="AC16QF_09845"
                     /inference="COORDINATES: protein motif:HMM:NF017021.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR6448899.1"
                     /translation="MGWLRATLIAVLAVVVALGAMAVWLTQADLSFLKPQVERLIAEQ
                     TGRAFTIGGEFSLNAGRRTAIVAEDVRLGNAEWASDPEMARIGRLRLELDLLSLLRGP
                     ARIHLIELEDASVLLENPADRDGNWVISADEPDAADSLTLPVLGDIVVDALSVTYLDP
                     ELEGPLSIEVVDARQHFDPETDGLSGSLQATVNGRSVVVSSHVSPLPAVIAGEDFDFE
                     LSLIAKALTLTASGHMDDLGRPRRPALKVNLMSPDVDLITDALGAEEFGDGGLDLNIE
                     LTPGTDTVALTATGDLAGLRVRVDAAADDLSSMRESSLNLSVQGPRFGRVMDVFGLEG
                     LPDDPFQASGQVKRSGPVLTLSDVLLEVEGTRLEIEGQTRGFPGIDAASLRLRLEGRE
                     LGRFQRFLSLPDWAVGPFTVAGSIEHSEGQTDTLDLAAATSLANVSLRGPIGDPPEYG
                     GTELSLTASGRDLRALVKVTESLEPTALEAVPAKPFSSGGRLTVKRRVFELSAVYLEI
                     EESRFDLEGSLSRRADLVGSRVTVTGRGGELESLIADAAGLDFKPAPFEVTATLERTP
                     ETLQIQDLLLTNERGGEVRLNAELGLPFPASRAADVNLVARGPDVSALPVQPQRLELE
                     PLPFEALLRASLRGDRLTVDKGSLVLGEAELDWRGTFDLPPEFSATESTLSVNVPDLA
                     ALGRLDGEPLPAAPLSLSARASGTSTTLRLEGIEAELGASALSGDLQLDVGGTRPRLQ
                     AKIHIPTLDVPVSEDAQEARRPPPADGRLIPDVALPVDGLDVMDGALELTVDELRFGD
                     LTFTQGFLSAVLEDGNLLVERLDIRPPSGRVQGRLAVRPEDESGTIDLELRGDRVALN
                     VAANLEPSAEPADQLVFDVDVYLRARGTDARALAASLEGEVSALSDGGRIHNRQLALI
                     FGGFGRELLGNINPFIKHDPYTDLSCVVFRFSATDGILKADPYLAIRSDKLNLVSSGF
                     ANLATERIDFTFNALPRLRLSISATELINPYVRVSGTLLKPTLSLDSRSAAVTGGAAA
                     LTGGLSLLAQATWKRTFGSRDACGKALEELRAEEGAAAAVTPP"
     gene            complement(9603..10613)
                     /locus_tag="AC16QF_09850"
     CDS             complement(9603..10613)
                     /locus_tag="AC16QF_09850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6448900.1"
                     /translation="MDNGRWTVASRPEDQVEEANFQFVSESVGEPEDGEFLIGTRYLV
                     VSPPARMALTSGGIARRPVPIGATMRGTGLGQVVASRHPGFAEGELVIGDLGWQEYAI
                     SDGATRHPVRKVAARPGMPESTLLHVLGAGGATAYFGMIEYAKPHVGDTLVVSAAAGN
                     VGSLVCQLGRLQGCRVVGIAGSDEKCSWLTNDLGCAAAINYKREDVAAALDGACPDGI
                     DVYFDNVGGEILDAALARIAQGARVVLCGGTSQYNHDLDWYGPKNYFNLVYRQAEMAG
                     FYVYNFSHRFEEAFGRLESLLATGELKYAEDVLQGIGQTPAALIRVLKGENFGVQLVR
                     VA"
     gene            complement(10618..11508)
                     /locus_tag="AC16QF_09855"
     CDS             complement(10618..11508)
                     /locus_tag="AC16QF_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6448901.1"
                     /translation="MQRKDHIDTLAAVTLIVMNVLLGLNQSVVKLVNDGFAPVFQSGL
                     RSACAILPVLGYALVMRKRLSVTDGSLGLGMLNGLLFSGEFCLLFLALDYTSVARVSL
                     FFYVMPVWVTIGAHFLVPGERMTLLKAGGLVLALAGVALGLGIGSGSAGEQAWIGDLL
                     ALGGAIFWASIALLTRTTRLQRCSPEMNLLYQLAVSAVILLAVAPLFGDTVREPTPAI
                     LGMFAFQVIVIVAIGFVVWFWILSIYPVSHMASFSLLAPVFGVLFGWLIFDDQITPAF
                     VIALLLVGSGIVLINRSPAG"
BASE COUNT         2061 a         3889 c         3808 g         1846 t
ORIGIN      
        1 ggccctacgg gccccgtggg caacatgtcc ttgatcacca tcaccgagca tcaggtgggc
       61 tacgtcatcg cgatgctcga gcgcatgaag aagcgccggc tcgtggccgt ctccgcgcgc
      121 gaggacgcct accggaacta caaccaggcc atgcaggagg cggtgaagaa cacggtctgg
      181 atgacgggcg gctgcacgag ctggtacatc gacaagaccg ggatcccgaa cctgtacccc
      241 tgggagccga tgcgctatct gaagtccatg cgcaatcccg acttctcgga ataccgggag
      301 gaaaccgggc aggggaagga agcggcgctg gcagcggcct agagggtcag ggggcgacct
      361 cctcgcgcag cctgaacttg agcaccttac ccatgacgtt gcgcggcagc gactggaaga
      421 agcgcacttc ccggggatgc ttgtaacggg caagacgtcc ggcgaagcgc gcgatcagct
      481 catcttcacc caggtcggcc ccgtcccgca gcaccacgca ggcaaccggg acctcgcccc
      541 agcgctcgtc cggccggccc accaccgcgt actctgcgag ctcgggcatc tccgccagca
      601 cgttctcgag ctcggcgggg tagacgttct caccgccgga gatgaccacg tccttcttcc
      661 gatcgtccac gtagaagaac ccttcggcgt ccatgtggcc gatgtccccg gtatgaaacc
      721 agccgtcgga gaacgcggcg gccgtggtct ccgggtcatt cagatactcg cggaaaagat
      781 tcgggccttt cagaacgatc tcgccacgct cccccggccc gaggatgttt cccccatcgt
      841 ccacgatccg cgcctcgcag tgaatgaccg gtttgcccgc cgccccccgc ttgcgtcggg
      901 tctcggcgat gggcaggcag atggccgtcg gcgcgctctc cgtgagcccg tatatctgcg
      961 tggcgggcac cccgcggtcg tgccaagcgt cgagcagcgg gtccggtacg gtagacgagc
     1021 cggtgcccac catacgcagg gaggagaggt cggtctccga ccacgccggg tggtcgacca
     1081 tggcggtgat gaccgccggt accgccagga acatggtggg cttgagcgtc tcgatggccc
     1141 tgagggcctc acccggatcg aagcgccgca tcaggctcac gccggcccca atgtaaaaag
     1201 ccggcgtggt ctgaatgttg agaccacctg cgtggaagag cggcaggacg gtgagcacgt
     1261 ggtccgacgc ccgcatctcc tgagcgtgaa tcgcgttcag cgcgttgtag aaaagcgcac
     1321 gctggtcgtg cgccacgccc ttcggctggc cggtggtgcc ggaggtgtaa atcagcagca
     1381 cccgatcatc cagcgttccc acgtcttcga aaccgccttc ccttgcgtca ccgagcatgc
     1441 gctgaaactc gtgccaatgt ggctccggcg gcgcacccat ggccacccgc tccaggcccg
     1501 gcacatcgat cgcgttcgcc tgggcagcaa actcgtcttc aacgaagaga aagcgcgcgc
     1561 cgctgtgacg cagtatccac tcgtgttccg ggccggccag gcgattgttc agggggagca
     1621 ggctggccgc gttgcgggcg cacgcgaaca gcaaaacgat catctgctcg ctgttgaggc
     1681 cgagaaacgc caccacgtcg ccgcgccgga tgcccaggcc gtcgcgcagc agaaaggcca
     1741 tgcgatccac ggcggcgtcg agctcagcgt aggtgtatgc agtctcgccc agacggagcg
     1801 ccgggcggcc gcctgcccag tcagcgttgc agcggatcca gtcgccgaga ccgttcaacg
     1861 cctcagctct ccagactttc ccgcccgagg cgttcgatgc agatgtcggg cgtccagggg
     1921 agcatgagcg agccgcagcg ggaatcccgg taaagctgct ccagccgcag ggatttgaac
     1981 agcgagcgcc cgccgcacac gcgaatggcc tgccggcaga tgtcgttggc aaactccatg
     2041 accgtgtact gagcggcgta agcgcgcagc cgctcgtctt tgctcggtcg gtaggcggcc
     2101 tccccgatgg ctttctcgaa gagcgcccgg gcctgctcca gcttgatgcg catctcggcg
     2161 acgcccacct gcttgaccgg gttgtcgcgg gcgccgcccg ccggcgggcc gccttccacc
     2221 tccccgcgca ggtactggca ggtgaaatcg aaggcggctt cagcgatgcc gaggtaggtg
     2281 gggcagaggg tcatgaacat gtgcggccag cccagcgccg cctggtagta gcagccctgg
     2341 ggcagaagct ggcccgaatc cggcacgaag acgtcctcga gaacgaggct gcgggagacc
     2401 gtgccgcgca tgccgagcac gtcccagtca ccggcaacgg agaacccgct ggcgctggca
     2461 ggcaccgcca gcagcagcgt gttgcggacg tccggctcgc ggccgtcggt gaggtcggtg
     2521 cagagcacgc cgtagaagtg cgccgagccg gaaagggacg caaagtgctt gcggccgttc
     2581 accagccagc caccgtccac cttcacggcg gatgtatcga aggccgcctt gccggccgcg
     2641 gcggcgctgt tgggctccga gaagggctgc gcgaagatgt ggccttcctc gatcaccccg
     2701 gcatagattt ccgctcggcg cgactcgtgg gccgagcgct cggcgtcgct catgggcagg
     2761 tcgtcggcca gctgacccga ccagagcatg gtggaagtgt gcatgttgaa ggtgagcgcc
     2821 gtggcgccgc accagcgccc gagctcaacg gaaatgctgg cgtaggtgcc gaaatcggcg
     2881 ccgagacccc cgtagcgccg gtcgatggcg agaccgagga aaccggcttc gcgcaggtcc
     2941 tcgtagttct cgtccgggaa tcgcgcttcc tcgtcatagg tccgccggcg ggggagaaag
     3001 cggtccctgc ccagctcgcg ggcaataccg atcagttcct cagtcttcgt catcacagct
     3061 gacccagaaa ttcgttcacg gcccgggtga aagcagcggg cccttccacg tacgcaagat
     3121 gacccgcgtc ctgcagacaa tcgtaacggc cggccgggat ctcggtcgcc atcttttcca
     3181 tggtgcgcgg cggcgccgtc tgatcgagct ccgctgccag gcacagcgtc ggggcgtcga
     3241 tctcaggaag ggtaccccgc tgatcgaaga cgaccaggca agtgagcacc tgccggtaaa
     3301 cgtccgcggg cagagggctc atggtggcaa cggcccgctc gatggcttcc gcacgggacg
     3361 gatcgccgaa catgccggtg atcagttctc tggcaaagtc cgccggggca cggccatcct
     3421 cgagcggctt gagccgggca tccagaaact gctgctgcca atcgctgccc ggcttgccga
     3481 agcgcgccat cgtctgggcg aggacgagag aacgcacgcg ccctggctcg gccagctggg
     3541 cgtcgaaggc atgccggttg ccgccgatgc catgcagaaa caccaccggc tcgccggaac
     3601 ccgcttcctt gaaacctgga acgctcaagc aagctcgcgc tgcaaggcac cgtccacaac
     3661 cacggcctcg ccgtccaggt gaacggagca gttgcgcagc ggcaggtcga agtggccgag
     3721 ggtatggcgc ccggcgactt cgttggcgcc ggtggagtat aggaaattgc cgccgaacgc
     3781 tcgcaactcc gtgccgttga agtcgttgcg atcgtacatg gtcatggcgt cccagcgggc
     3841 ggcgttgttc atgccccaac cgagatggct ggcggcgtag gcttcctggt ctccccacgc
     3901 cgcgaagtag ctgcgcatca gctcggcgtc cacccccgcc ccgtcgatct ccgtgacgta
     3961 gtcggcgtcg atgctgagcg ccacggggtt ctccaggtaa cgcttgaacg tcaggttaac
     4021 gtcaccgcgg tcgagaacga gaacgccgct caccgagccg gccgcgggga aacaaaggca
     4081 aagcccgccg ggccagtgac tcatgatgcc ggggcgcgca acgtagcccc agccgccgcc
     4141 ggccacggag ccttcgagag agacggtgag gtccgtcccc gcggccgagg tcaccttcat
     4201 ctggctcgcg ccgcgcagct tcctgatacc ggccttgacc cgggtctcca gggcctcgtc
     4261 gggctccagc cgctccaggg cctccgggtg ctcgttggaa atcatcagca cccgggcgcc
     4321 gcccttgagg atttccggca gctcgggcgc gtgaagcatg ccctcgacgg tgcagtcgac
     4381 gacgaatccc gccgacgaca gcgcggcgac gacggaacgc gagtggccaa tcacgtcggt
     4441 tgcgcccgtg gaacgaacgg gcaccggcgc cttctgtggc ggcgtaggga tcaccagatg
     4501 gtagggcctg gccccgagcc tgagcagggc gagctcggcg agctgtgcgt tggccggccg
     4561 gctctggctc tcagagagga tggcgacctc ctcgttctca gcaacggcgc accgctcgaa
     4621 cacccgtgcg aagcagtcga tccacttgcc ctcgatacgt tcctgcagca ttcgacgttt
     4681 ccccaaaatg ccgcattgta caggggttgc cggcgtccgc agcggtcccg gcagccgccc
     4741 ctgtgggtaa cgggtggggg gtattggggt ataaagacac cacccgtgcg aggagagagc
     4801 aatgagcctg atacgctacc cggtggttca gtacgcgtac gtcgtcgacg acatcgaagc
     4861 cgccgggtat cccgccgtga ccaccctgac gtccgccgcc cgggcggcct acatggacac
     4921 tcggcccatc ctgggctgct tcgtggagct ctacgaggac aacgccccgc tgcgggagac
     4981 cttcgcccac tggaaggccg tccacgacgc ctggaacggc gtcagcgacc cgattcgcga
     5041 tgcctgaccc tgaacagccc ggcgagctgc ccttccggcc agcggacgcg atccttcgac
     5101 ccgagtcatt tctgccggcc gctgacggca cggaagtctg cgctttcctc aatccctacg
     5161 acgcgcgcgg cgctgccagc gcgcactcgg cgggcgaagc gatcagcatg gccgccgggc
     5221 ggatagggcc aggtgtgcgt tccgccattc acgtccatcc ggcgctgacg cagatcacct
     5281 acgtcaccgc gggcacgctc accgcgcgga tgcgggatcc gggggagagc gaaccgtccc
     5341 gactcgaggt gcccactgga gccgccgtgc tgaccgaggc aggcaccctg ctgcagctgt
     5401 gcaacgaaac ggaccggacc gtggaagtgc tctacgtgtc cacgcccgca tacgttctgg
     5461 agaccgcggc tgacggcgcc atccgctacg aggacgcgtt gatcctgggc gcggactggg
     5521 acgcgcccgc ggtggcccac tggaacgtcg agaccgcaac acaggcgcgc agccgggcga
     5581 cggcgcgacg cgccgaggcg gccgggcgga tagccgggcg aaccaggcgc tgacgcgccg
     5641 cgacgatcca gctaagcccg cgactcggtg agcgcgcgtt gaaccagatg ctcgagggtc
     5701 tcgtagggtt gcgcgccgac gacgccgtag ccaccggcga cgaacgtcgg cacgccggtg
     5761 atcccgtatc gccgcgagcg cttccaatcc tcgtccacgg cattggagaa agtccgctgg
     5821 tcgatcacct cccgggcacc gccgggatcg agacctgcgg acttcgcgat gtcgacgagc
     5881 acgccggcat caccgatatt cagcccgtca acgaaatagg cgcgaaacag ggcgtcgtgt
     5941 atggcttcgc caccggggcg ggagtcggcc cacttcccga gttcctgagc gagccggctg
     6001 ttgaacgtgt gggtacgacg gccgtaagga agcccctcct cgtccatgag cgctttcatc
     6061 cgcacgtaca tggcatccag atcgatctcg cgaccggcga agagcgcttc gatggtgatt
     6121 ccctcctcgg gagtttccgg atgcaggggg aaggcaaccc agcgaacctc cagatcgaag
     6181 ctggacttca gtttttcaat acgcccggta ctgaggtaac accagggtca tatgtagtcg
     6241 ctgaagatct ccagcttcaa ctgatcggtc atctgcccgt cccgcttcga ctttgcgcca
     6301 tcgtaacccg tcgttgcccg ccacgcccat ctctgttaaa tccctcgttc gtcccgtcat
     6361 ccggcgggct tgggttcgag gaggggaagc gttcatgggt tggctgcggg ccacactgat
     6421 tgcggtgctc gctgtggtcg tcgccctcgg ggcgatggcc gtgtggctca ctcaggcaga
     6481 tctgagtttc ttgaagcccc aggtggaacg cctgatcgcg gagcagacgg gccgcgcttt
     6541 caccatcggc ggtgagtttt ccctgaacgc ggggcggcgc acggcgatcg ttgccgagga
     6601 tgtacggctg ggcaatgcgg aatgggcgag cgacccagaa atggcgcgca tcgggcggct
     6661 ccggttggag ttggatctgc tctccctgct gcgcggacca gcacgcatcc acctcatcga
     6721 gttggaagac gcctccgtgc tcctggagaa cccggccgac cgggatggca actgggtaat
     6781 ctctgcagat gagcccgacg cggccgactc gttgacgctg cccgtgctgg gggacatcgt
     6841 cgtcgacgcc ctgtccgtta cctacctgga cccggaactc gaggggccgc tcagcatcga
     6901 ggtggtcgac gcccgccagc atttcgaccc tgagaccgac ggcctcagcg ggtccctcca
     6961 ggcgaccgtc aatggtcgct cggtggtcgt cagctcccat gtcagtcctc tgcctgccgt
     7021 gatcgccggg gaggacttcg acttcgagct ctccctgatc gccaaagcat tgaccctgac
     7081 cgcctcgggg cacatggacg acctcgggag accccgccgt ccggccctga aggtgaacct
     7141 gatgagcccc gacgtcgacc tgatcaccga tgccctgggc gcggaagagt tcggagatgg
     7201 cgggctggat ctgaacatcg agctgacccc gggcaccgat acagtggcgc tgacggcgac
     7261 cggcgatctg gccggtctcc gggtgagggt cgacgccgcg gccgacgact tgagctcgat
     7321 gcgcgaatcg agcctgaacc tgagcgttca gggaccccgc ttcgggcgcg tgatggacgt
     7381 tttcggtctg gaggggctgc cggacgatcc gttccaggcg tcgggtcagg taaaacgctc
     7441 cggccccgta ttgacgctga gcgacgtgct gctggaggtg gaaggcaccc gcctcgagat
     7501 cgaagggcag acgcgcggat ttccgggaat cgatgcagcg tccctgaggc ttcgcctcga
     7561 gggtcgggaa ctcggccggt tccagcgttt cctctccctt ccggactggg cggtgggtcc
     7621 ctttacggtt gcgggttcca tcgagcactc ggaaggccag acggacaccc tggaccttgc
     7681 cgccgccacc tcgctggcca acgtcagcct gcgaggcccc atcggcgatc cacccgagta
     7741 cggcggcacc gagctgagcc tgaccgccag cggcagggat ctcagagcgc tggtgaaggt
     7801 gactgaaagc ctggaaccga ctgctctgga ggcggttcct gccaagccgt tcagttccgg
     7861 cggtcgcttg acggtaaagc gccgcgtctt cgagctgagc gccgtttacc tcgaaatcga
     7921 ggaatccaga ttcgacctgg aaggttcgct ctcccgcagg gccgacctcg tcggcagccg
     7981 tgtcaccgtc accgggcgcg gcggagagct cgaaagcctg atcgccgacg ccgcgggcct
     8041 cgatttcaag ccggccccct tcgaagtcac cgccacgctg gagcgcacgc ccgaaacgct
     8101 tcagatccaa gaccttctgc tcacgaacga gcgcggcggc gaggtgcgcc tgaacgcgga
     8161 gctcgggcta cctttcccgg cgagcagggc ggcagacgtc aatctggtgg cccgggggcc
     8221 cgacgtctct gcgctaccgg ttcaacccca acgcctggaa ctcgagccgc tgccgttcga
     8281 ggcgcttctg cgcgccagcc tgcgcggcga ccggctcacc gtagacaagg ggagcctggt
     8341 cctcggagag gccgagctcg actggcgcgg caccttcgac ctgcccccgg agttctccgc
     8401 caccgaatcg accctgtcag tgaacgtccc cgacctagcg gcgctgggca ggctcgatgg
     8461 cgagccgttg ccggccgcgc ctttgagcct gagcgcgcgc gccagcggca cgagcaccac
     8521 gctgaggctc gaaggcatcg aggcggagct cggcgcctca gcgctctccg gcgatcttca
     8581 gctggacgtc ggcggtaccc ggccgcgact gcaggcaaag atccacatcc cgacattgga
     8641 cgtgccggtg agcgaagacg cgcaggaggc gcggcgcccg ccgccggcgg acggtcggct
     8701 gatccccgac gtcgctctgc cggtcgacgg tctggacgtc atggatgggg cgctcgaact
     8761 gaccgtggac gagctgagat tcggcgacct gacgtttacc caggggttcc tgtccgccgt
     8821 gctggaggac ggcaacttgc tggtggaacg cctcgacatc cgcccaccca gcggccgggt
     8881 gcaaggccgt ctagcggtcc ggccggaaga cgaatccggc accatcgacc tcgagctccg
     8941 cggtgatcgg gtggcgctga acgtggcggc aaacctcgaa ccgtccgcgg agcctgccga
     9001 ccagctcgtg ttcgacgtcg acgtgtatct gcgcgcccga ggcaccgatg cgcgggctct
     9061 ggcagcatcg ctggaaggcg aggtcagcgc gctgagcgac gggggccgga tccacaaccg
     9121 acagctcgcg ctgatcttcg gcgggttcgg gcgcgagctg ctcggcaaca tcaatccttt
     9181 catcaaacat gatccttaca cggatttgtc ctgtgtggtg tttcgattct ccgcaacgga
     9241 cggcattctg aaagccgatc cgtacctcgc gatacgctcc gacaagctga acctggtcag
     9301 cagcggattc gccaacctgg ccaccgagcg catcgatttc accttcaacg cgctgccaag
     9361 actccgactg agtatcagcg ccaccgagct gatcaacccc tacgtgcggg tgagtggaac
     9421 cctgcttaag ccgaccttga gcctggacag ccgaagcgcg gcggtgacgg gcggcgcggc
     9481 ggcattgacc ggaggtttgt ccctgctcgc tcaggccacc tggaagcgca ctttcggctc
     9541 ccgcgacgcc tgcggcaagg ccctggagga actgcgcgcg gaagagggcg cagcagcagc
     9601 cgtcacgcca ccctgaccag ctgcacgccg aagttctcgc ctttgaggac gcgaatcagc
     9661 gctgccggcg tctgcccgat tccctgcagc acgtcctcgg catacttgag ctctcccgtc
     9721 gcgagcaggg actccagccg cccaaaggcc tcctcgaagc ggtgggagaa gttgtagacg
     9781 tagaatccgg ccatttcagc ctggcgatag accagattga agtagttctt cggtccgtac
     9841 cagtccaggt cgtggttgta ctgagacgtt ccgccacaca gcaccaccct ggcgccctgg
     9901 gcaatgcgcg caagcgcagc gtcgaggatc tctcccccga cgttgtcgaa gtagacgtct
     9961 atgccgtcag ggcaggctcc gtccagggct gcggcgacgt cttcacgttt gtagttgatc
    10021 gccgccgcgc agcctagatc gttggtgagc caggaacact tctcgtcgct gcctgctatc
    10081 ccgactaccc ggcagccctg cagccggccc agctgacaga ccagcgagcc gacgttgccc
    10141 gccgccgccg agaccaccag cgtgtcgccc acgtgcggct tggcatactc gatcatgccg
    10201 aagtaggcgg ttgcaccccc ggcccccagc acatgcagca gggtgctttc cggcatcccc
    10261 ggtctggctg ccaccttccg cacggggtgg cgcgtggcgc cgtccgagat ggcgtactcc
    10321 tgccagccga gatccccgat gacgagttcg ccctcggcaa accccggatg gcgggaggcg
    10381 accacctggc ccagccccgt tccgcgcatg gtggccccga tcggcactgg acggcgcgcg
    10441 atgccgccag aggtgagcgc catccgggcc ggcgggctca ccaccagata gcgcgtgccg
    10501 atgagaaact cgccgtcctc cggctcgccc accgactcgc tgacgaactg gaaattggcc
    10561 tcttccacct ggtcctccgg gcgcgacgcg accgtccatc tgccgttgtc catccactca
    10621 tcctgccggg ctgcggttga tcagcacgat accagatcca accagcagca aggcgatcac
    10681 gaacgctggc gtaatctgat catcgaagat cagccatccg aacaagaccc cgaacaccgg
    10741 cgccagcagg ctgaaggaag ccatgtggga gacgggatat atcgacagga tccagaacca
    10801 gaccacgaag ccgatggcga cgatcacgat gacctgaaac gcaaacatcc cgaggatggc
    10861 cggtgtgggc tcacgcaccg tgtcgccgaa cagaggcgcc accgccagca gaatcacggc
    10921 ggatacggcg agctggtaga gcaggttcat ctccggcgag catcgctgca gccgggtggt
    10981 acgggtgagc agcgctatgc tcgcccagaa gatcgcgcct cccagcgcca gcaggtctcc
    11041 gatccaggcc tgctcgccgg ccgagccgga gccgatgcca agacccaggg ctacgccggc
    11101 cagcgcgagg acaaggccgc cggccttgag cagcgtcatc cgctcgcccg gaacgagaaa
    11161 gtgggcgccg atggtcaccc ataccggcat gacgtagaaa aacagggaga ccctggccac
    11221 gctggtgtag tccaaagcga ggaagagcag gcagaactcg ccggagaaaa gcaggccgtt
    11281 gagcatcccc aggcccaggc tgccatcggt gaccgagaga cgcttgcgca tcacgagcgc
    11341 gtagccgaga acgggcagga tggcacacgc ggagcgcaat cccgactgaa acacgggagc
    11401 aaatccatcg ttgacaagct tgaccacgga ctggttcagt cccagcagga cgttcatgac
    11461 gatgagcgtt acggccgcca gagtgtctat gtggtctttg cgctgcatgg ggcagcatga
    11521 tagccgcagt gaaccgaacg ggtatcaacg aaacccgggg agggagcaag tgaagaggat
    11581 cgtcatctgt gcggatggca catg
//
LOCUS       DBNHLM010000213        12722 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1467822, whole genome shotgun sequence.
ACCESSION   DBNHLM010000213 DBNHLM010000000
VERSION     DBNHLM010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12722)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12722)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12722
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1467822"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="AC16QF_09860"
     CDS             <1..682
                     /locus_tag="AC16QF_09860"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154043.1"
                     /note="catalyzes the formation of malate from glyoxylate
                     and acetyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HIR6448902.1"
                     /translation="ESGLVGRAQIGKGMWTMPDEMNKMVATKQSHPEAGANTAWVPSP
                     TAATLHATHYHQVSVFERQQALAAREPASLDDILRIPLLGGRNLSAEEVQAELDNNAQ
                     GILGYVVRWIDHGVGCSKVPDINDVGLMEDRATLRISSQHIANWLHHGICSREQVLGT
                     MQRMAAVVDGQNAGDAAYRNMAPDFDASVAFQAACDLVFKGLEQPNGYTEPVLHARRR
                     EAKARFGS"
     gene            complement(715..2487)
                     /locus_tag="AC16QF_09865"
     CDS             complement(715..2487)
                     /locus_tag="AC16QF_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR6448903.1"
                     /translation="MDVQLLTYLFVGLSFALYIGIAIWSRAASTREFYVAGGHVHPVA
                     NGMATAADWMSAASFISMAGIIAFMGYDGTVYLLGWTGGYVLLALLLAPYLRKFGEFT
                     VPDFIGTRYYSNTARVVAVVSLIVISFTYVAGQMRGVGIVFSHFLGVEIELGVVIGMA
                     VVFMYAVLGGMKGITYTQVAQYCVLIFAYLVPAIFISMLITGIPIPQIGLGAAVNDGS
                     GVSVLARLDATLVELGFGPYTQGSKSTIDVFAITMALMIGTAGLPHVIVRFFTVPKVS
                     DARKSAGYALLFIALLYTTAPAVGAFARLNFVETVHGTGYADVAGWFKSWEDVGLIGW
                     LDKNEDGAIQYAPGAPFEGKPEFDGVRGPDGQRLIRNAPTGNANEVYVDRDIMVLANP
                     EIAALPPWVIALVAAGGLAAALSTAAGLLLVISTSVSHDLIKKTMARGISDRQELLYA
                     RIAAALAIAVAGYLGINPPGFVAQVVAFAFGLAAASLFPAILLGIFSKRMNREGAIAG
                     MVVGLTFTFAYIVYFKFVAPGDNNAAHWWLGISPEGIGMVGALANFATAAIVSRFASP
                     APEDVQQLVEKIRIPRGVETPHTH"
     gene            complement(2503..2769)
                     /locus_tag="AC16QF_09870"
     CDS             complement(2503..2769)
                     /locus_tag="AC16QF_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HIR6448904.1"
                     /translation="MSSQRDDHAYWRANLKLGAVLLGIWFVVSFGFGILLVEPLNRIP
                     LFGFKLGFWWAQQGAIFVFVILIFVYSRLMKRIDRRYGVHDDED"
     gene            complement(2817..3731)
                     /locus_tag="AC16QF_09875"
     CDS             complement(2817..3731)
                     /locus_tag="AC16QF_09875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6448905.1"
                     /translation="MKLSVEFPSVAYREGPEGIIRLAKAIEDIGYDQLDMFDHVVMGY
                     PTETRAAPIYPPQMPIMEALMVLAYAAAVTTRIGLGTEVLVLPQRQPVLVAKQISTLD
                     TLSGGRVRLGVGVGWQASEYDALEESFANRGRRMDEAIRLLRAYWSEEKVEFAGEYYT
                     SHAMAMEPKPPQGGQLPIWIGGLAPAALRRVGELGDGWLATAFTDPDRARAAVATIHR
                     HAEDAGRDPAAIGLQQMLDVPPRDDSGKGFYADLDQVEARALAVKEMGFEWGALNATA
                     IFQSGARSVDAMIDTLGALHERLRAATA"
     gene            3826..4578
                     /locus_tag="AC16QF_09880"
     CDS             3826..4578
                     /locus_tag="AC16QF_09880"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6448906.1"
                     /translation="MSQVQPIRPAATVILVRDAADGFEILMLKRTSGAAFAGGMFVFP
                     GGRVDGDDHLHRYDEHRVGPSDVQQAQQRALGAEWRGYWIAGIRESFEEAGLLLAYDR
                     AGELLAYDDDAVEERFQSYRGPLHAGQVSLETICRQEGLRLAVDRIHFHSRFVTPLGR
                     PRRFDTRFFIAEAPPGQTGRHDDKETVDSIWISPGEALARHAVEEFGLMGVTKLQLEG
                     LAGHRSKADVLAAAARRADFPTRRPVLPPSER"
     gene            4669..5625
                     /locus_tag="AC16QF_09885"
     CDS             4669..5625
                     /locus_tag="AC16QF_09885"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6448907.1"
                     /translation="MELDYSNIDPELRPALDFLPAELDLITRETLHATRAVLAAAPAP
                     DVETDVEVTEQIIAGPGGDLRLLIYRSPVAEGGPGVLWLHGGGYILGAVDDDRARLVA
                     ESLGCTAVSVDYRLAPEHPFPAAVEDAYAALCWLAGNAGALGVDAERLSIAGLSAGAG
                     LTAGLALLNRDRGGPAVAFQLLLSPMLDNLHDTASGRIVNHPTWSRQTSLNAWEMYLD
                     GTPGLDASPYAAPSRATDLSGLPEAYVSVGAEDLFRDECIDYARRLHEHGVATELAVF
                     PGMYHVAENFAPQARVCQRLHGSYLRALADGLGVSRGAADGS"
     gene            5660..6880
                     /locus_tag="AC16QF_09890"
     CDS             5660..6880
                     /locus_tag="AC16QF_09890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6448908.1"
                     /translation="MTIRSYDEDRDLKAIERIWFECGWLENSRQAAYLKDFLAAGDCL
                     TATIDDTAECAVHVSPGALQYVDEQLELCAVTAVTTSRVARKQGFARELTARQLARGA
                     DDGAEIAALGMFEQGFYDQLGFGTGSYEHEFTFDPATLTVDVPFRPPSRLDRGNWRDI
                     HASLAKRLRGHGGCVLRPSYLIKAELGFSEDGFGLGYFDGSDLTHFFWGEPRGEHGPY
                     RIHFLAYRDAEQLMELLALMRSLGDQVSSIVLLEPPEVQLQDLLDRPFRARRNTRASA
                     HANQHRSIAFWQARVLDVEACVAVRHWPGDEVDFNLVLTDPLSEILAGEAWSGVAGEY
                     VVHVGADSSARSGFEAGLPVLHAGVGAFTRLWLGVVGASALALSDELTGPTELLGRLD
                     EALCLPPARTTWDF"
     gene            6877..7632
                     /locus_tag="AC16QF_09895"
     CDS             6877..7632
                     /locus_tag="AC16QF_09895"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR6448909.1"
                     /translation="MTIRLEIDGIEVELRRNSRRRTRIGMAFDPSGFVIMDAPPNVGE
                     NEVREVVAEHHRWLRVRLDKARDAVHEVRALNYVAGELVHYLGQAYRLEVRAGGEVRV
                     ELSAPAHSQLPLFADVAFGTLQVTTPEQSPDGVKTAVCDWYSAQGDRVFAERLAGWSE
                     LPWLNGTTPRWRHTFMRSQWGSCSARGKISLNTHLVKAPVSLVDYVILHELCHLEHHD
                     HGRRFYALMERHMPDWERRRAALDRFLPVLLHD"
     gene            7639..8700
                     /gene="rsgA"
                     /locus_tag="AC16QF_09900"
     CDS             7639..8700
                     /gene="rsgA"
                     /locus_tag="AC16QF_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR6448910.1"
                     /translation="MNGARPTLPELGWTPFFAEQLSPEEADRLIPARVFGVQRTHITV
                     MAEEGELSMPLGGRWFQLDPQDRPTVGDWVLVSEQRDSVVRLLERRSLLKRLSPGRTG
                     AVQLIAANVDTLFLVTSCNQDFNPRRLERYLALAYESGIEPVVVLTKADLCDDLERYV
                     DGARRIVPGLEVVALDARDRAQLAPLASWLATGRTVALLGSSGVGKSTLVNTLSEAQV
                     QRTAQIREDDGKGRHTTSHRSLHPVPGGGLMLDSPGMRELGMADAELGVRQMFEDVEA
                     LAHQCRFADCAHDSEPGCAVKAALEAGELTEERLESYRKLSREEAVNAETIAERHARS
                     RATDKLHRRVQDASPKRRS"
     gene            8761..10152
                     /locus_tag="AC16QF_09905"
     CDS             8761..10152
                     /locus_tag="AC16QF_09905"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR6448911.1"
                     /translation="MADLLAEQLRLQPVSVTRRMSASLPGLRRLAQEKGLKIHHLGAG
                     YPHPEVTDPRSFIAHREAYFAHLEEREGLNDPRALPEYLRESFSYTDTLGPAGTRAAF
                     ARVYGRDWGVTMDPGRLIPTVGATGGINLACSMFERPGVPLAYITDAPTYAGFNARAT
                     LCQHAAIYSVPMDDEGPLPDVLRDQIRAARADGRFVPFYYTVPDGHNPAGFSFSQARR
                     EAILRVVREEGILILEDAPYVYINFAPAANRPRPFLAMDPRCTIHLFTGSKIGFPGPR
                     VGFLYSEAELAIAGGRTASLTEFALTEASADMLFQNPAALLGFEALLHDDDFSERRSL
                     WPVAETKLDVYRENRSIMLDGLERELGGHRDLFHWTDPKAGFFTVFTFLGPGIRTDDA
                     FVNAMVADHGVVVIPMYDFYPEDARRRDPDAGFDQLRLSFCFSESLGEQRRTDLEEAV
                     AAFCAAAKKLAGV"
     gene            complement(10173..11546)
                     /locus_tag="AC16QF_09910"
     CDS             complement(10173..11546)
                     /locus_tag="AC16QF_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448912.1"
                     /translation="MLEAVNVSAEVVAAILGISITVVAIIVQLAATRYNHHITEMFVR
                     EPINVVVQAFFVLTTVLCIWVAALGEAELNATWNTVTLVTVTVALAALLPYFGYVFAF
                     ISPVGIIRRIRRKAEHAVAAGHRAETLRTIDQLQDVARSAIDHGDRAIAMAAVEALSD
                     LFRHYRSVRNRLPEGWSRVDGIVREDPDFVALEPSALDDLDTSGLWVEVKIMHQYVSI
                     IRLSVMKMHDVAGRVGIALRQTAIEQIDDPLVLEASFRGMNSLLRASLNARDIRTTFH
                     LLSQYKAVAEDLVSMGHDEDALAAAEHIQSYGQGAFTMNQPFLLEVAAFDLGELIRLA
                     HKNQSAALDRMLDGFLELDHEIKYESQEESLLGVRRAQMQLGAYLISQGDLARAQRVA
                     DDLSSEGASRLDRLQAGLLSDERALFWEFNPRGVNFSYLEPELRPHLNTLMDMVRNEP
                     RRVAPTP"
     gene            complement(11701..12045)
                     /locus_tag="AC16QF_09915"
     CDS             complement(11701..12045)
                     /locus_tag="AC16QF_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HIR6448913.1"
                     /translation="MRRAIGFLILTTVTTVPAWAGDKLEAAVGGGAGGAIGAVVGDEL
                     GDRKGAIIGSAVGAAIGTAIATDGNDGGPDHHHVEAHTAPASTVHVEVGSAGGHPAGR
                     HCPPGQAKKGRC"
     gene            12216..>12722
                     /locus_tag="AC16QF_09920"
     CDS             12216..>12722
                     /locus_tag="AC16QF_09920"
                     /inference="COORDINATES: protein motif:HMM:NF018712.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HIR6448914.1"
                     /translation="MTDHWHFHSDTFGNCNCAQNCGCQFNLPSTHGFCQFVEGGHIRD
                     GSFNGMNISGFNWAFMMIWPGEIAEGNGRRQIVIDERANDQQRAAIEKIVSGEVGIPG
                     GNHFSVFGSTCSESLETLYLPIEFEMDIAARRARLAIPGLIEARGEPMMDGFSGSEFH
                     IALARPKGS"
BASE COUNT         2084 a         4152 c         4344 g         2142 t
ORIGIN      
        1 cgagtccggt ctcgtgggaa gggcgcaaat cggaaagggg atgtggacga tgccggacga
       61 gatgaacaag atggtcgcca cgaagcaatc ccaccccgag gcgggcgcca atacggcctg
      121 ggtgccctcg ccgacggcgg ctaccctgca cgccacccac tatcatcagg tgagcgtttt
      181 cgagcgtcag caggcgctgg ccgcccggga gccggcgagc ctggatgaca ttctccgcat
      241 cccgctcctc ggcggacgca acctgtcggc agaggaagtg caggccgagc tcgacaacaa
      301 cgcccagggc attctcggct atgtggttcg atggatcgat catggcgtgg gttgctccaa
      361 ggtccctgac atcaacgacg tcggtctcat ggaggatcgg gcaacgctgc gcatctcgtc
      421 tcagcatatc gccaactggc tgcatcacgg catctgcagc cgcgagcagg tgcttgggac
      481 catgcagcgg atggccgccg tggtggacgg gcagaacgcg ggcgatgccg cctaccgcaa
      541 catggcgccg gacttcgatg ccagcgtggc gttccaggcg gcctgcgatc tcgtcttcaa
      601 gggcctggag cagcccaacg gctacaccga gccggttctg cacgctcgcc gacgcgaggc
      661 caaggcccgc ttcggcagct agccctctcg ctggcccggt accgggccgg ccgatcagtg
      721 ggtgtggggc gtctccacgc cccgcgggat gcggattttt tccaccagtt gctggacgtc
      781 ctccggcgcc gggctcgcga accggctgac gatggccgcg gtcgcgaagt tggcgagcgc
      841 ccccaccatg ccgatgccct cgggagagat gcccaaccac cagtgggcgg cgttgttgtc
      901 gccgggcgcg acgaacttga agtagacgat gtaggcgaac gtgaacgtca ggcccaccac
      961 catccccgca atggcgcctt cccggttcat ccgtttgctg aagatgccga gcaggatcgc
     1021 cgggaacagg gaagcggcgg ccagaccgaa ggcgaaggca accacctggg cgacgaagcc
     1081 gggcgggttg atgcccagat agcctgcgac ggcgatggcc agcgccgcgg cgatgcgggc
     1141 gtaaagtagc tcctgccggt cgctgatgcc ccgcgccatg gtcttcttga tcaggtcgtg
     1201 ggagacgctc gtcgagatca ccagcagcag cccggcggcg gtggagagcg cggcggcaag
     1261 gccgccggcg gccaccaggg cgatgaccca gggcggcagc gcggcgatct ccggattggc
     1321 caggaccatg atgtcgcggt ccacgtagac ctcgttggcg ttgccggtcg gcgcgttgcg
     1381 gatcagccgc tggccgtccg ggccgcgcac cccatcgaat tcgggtttgc cctcgaacgg
     1441 ggcgccgggc gcgtactgga tggcgccgtc ctcgttcttg tccagccagc cgatgagccc
     1501 gacgtcctcc cagctcttga accagccggc cacgtcggcg taccccgtgc cgtgcacggt
     1561 ttccacgaag ttcagccggg cgaacgcgcc caccgccggc gcggtggtgt agagcagggc
     1621 gatgaacagc agcgcgtagc cggccgattt gcgggcgtcg gagaccttgg gcacggtgaa
     1681 gaaacggacg atgacgtggg gcaggccggc tgtgccgatc atgagggcca tggtgatggc
     1741 gaagacgtcg atggtgctct tgcttccctg ggtgtacggg ccgaagccga gctcgaccag
     1801 cgtcgcgtcc agcctggcca gcacgctgac cccggaaccg tcgttcaccg cggcgcccag
     1861 gccgatctga gggatgggaa tgccggtgat gagcatggat atgaagatcg ccggaaccag
     1921 gtaggcgaag atcagcacgc agtactgggc aacctgggtg taggtgatgc ccttcatgcc
     1981 ccccagcacg gcgtacatga acaccacggc catgccgatg acgacgccca gctcgatctc
     2041 caccccgagg aagtgggaga agacgatgcc cacgccgcgc atctgccctg cgacgtaggt
     2101 gaaggagatg acgatgaggc tcaccacggc gacgacgcgg gccgtgttgg agtagtaacg
     2161 cgtgccgatg aagtccggga ccgtgaactc gccgaacttg cgcaggtagg gcgccagaag
     2221 cagcgcgagc agcacgtacc cgcccgtcca gccgagcagg tagacggttc cgtcgtaccc
     2281 catgaaggcg atgatgccgg ccatggagat gaacgaggcc gcgctcatcc agtcggcggc
     2341 ggtagccatg ccgttggcca ctggatggac gtgcccgccc gcaacgtaga actcacgggt
     2401 cgatgcggct cgggaccaga tggcgatgcc gatgtagagc gcgaagctca ggcctacgaa
     2461 gagataggtg agtagctgga cgtccactgg ccgctgctgc cgtcagtctt cgtcgtcgtg
     2521 gacgccgtag cgccgatcga tgcgcttcat gagccggctg tagacgaaga tgaggatgac
     2581 gaagacgaag atcgcgccct gctgggccca ccagaatccg agcttgaagc caaacaaggg
     2641 gatgcggttc aacggctcga cgagaagaat accgaagccg aaggagacga cgaaccagat
     2701 cccgagcagc accgccccga gtttcaggtt cgcccgccag taggcatggt cgtcgcgttg
     2761 agatgacatc ggttacccct cccggggggc cattgtaagg cctgcgcggg caataatcag
     2821 gcggtcgcgg cgcgcagccg ctcgtgcagc gcgccgaggg tgtcgatcat cgcgtccacg
     2881 ctgcgggcgc cggactgaaa gatggcggtg gcgttgagcg ccccccactc gaaccccatc
     2941 tccttgacgg ccagggctct ggcctccacc tgatccaggt ctgcgtaaaa gcccttgccc
     3001 gagtcgtcgc gggggggcac gtcgagcatc tgctgaaggc cgatggcggc ggggtcacgt
     3061 ccggcgtcct cggcgtggcg atggatggtc gccacggcgg ctctggcgcg atccgggtcc
     3121 gtgaacgcgg tggccagcca gccgtctccg agttctccca ccctgcgcag cgccgcaggt
     3181 gccagcccgc cgatccagat cggcagctgg ccgccctggg gcggcttggg ctccatggcc
     3241 atggcgtggg aggtgtagta ctcgccggcg aactccacct tttcctcgga ccagtaggcg
     3301 cgcagaaggc ggatggcctc gtccatgcgc cggccgcgat tggcgaagct ctcctcgagg
     3361 gcgtcgtact cggaagcctg ccagcccacg cccacgccca gccgcacccg gccgccggag
     3421 agcgtgtcca gggtgctgat ctgcttggcc accagcacgg gctggcgctg tggcagcacg
     3481 agcacctcgg tgccgagccc gatcctggtg gtcacggccg ccgcgtaggc gagcaccatg
     3541 agagcctcca tgatcggcat ctgcggtggg tagatgggtg ctgcgcgcgt ttcggtgggg
     3601 tagcccatca ccacgtggtc gaacatgtcc agctggtcgt agccgatgtc ctcgatggct
     3661 ttcgccagcc ggatgatgcc ctccgggcct tcccgatagg cgacgcttgg aaactcgacg
     3721 ctcagcttca tgtgaaatcc ccggatggtc aggcgggcaa agataccgcc tgtggcggtc
     3781 cgggtaaaaa ccggatagcc tgccgcgttt cgatcgggag gcctgatgag tcaggtgcag
     3841 ccaatacgtc cggcggcaac ggtgatcctc gtgcgcgatg cggcggacgg gttcgagatc
     3901 ctgatgctca agcgcaccag cggtgccgct tttgccggcg ggatgttcgt ctttcccggc
     3961 gggcgggtcg acggcgacga tcacctgcac cgctacgacg agcatcgcgt ggggcccagc
     4021 gacgtgcagc aggcgcagca gcgggcgctg ggtgcggaat ggcgcgggta ctggatcgcc
     4081 ggtatccgcg agagcttcga ggaggccggc ctgctcctgg cgtacgaccg ggcgggcgag
     4141 ctgctggcct acgacgacga tgcggttgaa gagcgcttcc agagctatcg gggcccgctg
     4201 catgccggtc aggtcagcct ggaaaccatc tgccggcagg aaggcctgcg gctggcagtg
     4261 gaccgcattc atttccacag ccggttcgtg acgccgcttg gccggccgcg acgtttcgat
     4321 acgcgcttct tcatcgccga ggcgccgccg gggcagaccg gtcggcacga cgacaaggaa
     4381 accgtggata gtatctggat ctcgccgggc gaagcgctgg cgcgccacgc cgtggaggag
     4441 ttcggcctga tgggcgtgac caagctgcag ctggaaggac tggccggaca ccggtccaag
     4501 gcggatgtgc tcgctgccgc cgcgcgcagg gccgatttcc cgacgcggcg gccggtgctg
     4561 ccgccgagcg agcggtgagg ccttgatcaa ctgagcgtag gtaagatatg gcagtcggga
     4621 tctgcgggtt catgaccgtc gcgagcacta agcgagctgg agaaacacat ggaactcgat
     4681 tactcgaata ttgatcccga actccggcct gcgctggatt ttctgcccgc ggagctcgat
     4741 ctgatcacgc gtgagactct gcacgctacc cgcgctgtgc tggcggccgc tcccgctccc
     4801 gacgtggaga cggacgtgga agtcaccgag cagatcatcg ccgggccagg cggcgacctg
     4861 cggctcctga tctaccggtc tcccgttgcc gagggcggcc cgggggttct gtggctccac
     4921 ggcggcggct acatcctggg agcggtggac gacgaccggg cacgcctcgt tgccgaatcg
     4981 ctcgggtgca cggcggtatc cgtagattac cgtctggcgc cggagcatcc tttcccggcg
     5041 gcggttgaag atgcctacgc tgcgttgtgc tggctggccg ggaacgccgg cgcgctcggt
     5101 gtggacgccg agcgcctttc cattgccggg ctcagcgcag gggcggggct gacggcgggg
     5161 cttgccctgc tcaatcgcga ccggggaggg ccggccgtcg cctttcagct tctgctttcc
     5221 cccatgctcg acaacctcca cgacaccgcc tcgggccgga tcgtcaacca cccgacctgg
     5281 tccaggcaga cgtcgctgaa cgcctgggag atgtacctgg acggcacgcc cgggctggac
     5341 gcctcaccct atgcggctcc cagccgcgca accgacctct ccggtctccc tgaggcgtac
     5401 gtatccgttg gcgcggaaga tctgttccgg gacgagtgca tcgactatgc ccgccggctc
     5461 cacgagcacg gggtggccac ggagctggcc gtgtttcccg gcatgtacca tgtggctgaa
     5521 aacttcgcgc cccaggcgcg ggtctgtcag cggctgcacg gaagctatct gcgcgccctg
     5581 gcggacgggc tgggggtgtc gcgtggcgcg gccgacggct cctgacgcat aatccgggtt
     5641 ttccgcgccc tgcgtgccta tgacgatccg cagctacgac gaagaccggg acctcaaagc
     5701 catcgagcgc atctggttcg agtgcgggtg gctggaaaat tcgcgtcagg ctgcttatct
     5761 caaggacttc ctcgccgcgg gcgactgcct caccgccacc atcgacgata cggcggagtg
     5821 cgcggtgcat gtttcccccg gcgccttgca gtacgtcgac gagcagctcg agctgtgcgc
     5881 ggtgaccgcc gtgactacca gccgggtcgc gcgcaagcag gggtttgccc gcgagctcac
     5941 ggcgcgccag ctggcccgcg gagcggacga tggtgccgag atcgcggcgc tcggcatgtt
     6001 cgagcagggc ttttacgacc agctcgggtt cggcacgggc agctacgagc acgagtttac
     6061 gttcgacccg gcgaccctga cggtagacgt gccgtttcga cccccgagcc ggctcgatcg
     6121 aggcaactgg cgcgacattc atgcctccct ggcgaagagg cttcgcggcc acggcggctg
     6181 cgtgctgcgc ccttcgtatc tgatcaaggc ggagctcggc ttctcggaag acgggttcgg
     6241 cctcgggtac ttcgacggat ccgatctcac ccacttcttc tggggcgaac cccgcggcga
     6301 gcacggaccc tatcgcatac acttcctggc ctaccgcgac gccgagcagc tcatggagct
     6361 tctggcgctg atgcgctccc tcggtgatca ggtgtcctcc atcgtcctgc tggagccacc
     6421 tgaagtgcag ctgcaggatc tcctggatcg gccatttcgc gcgcgccgca acaccagggc
     6481 ttccgcccat gccaaccagc accggtccat tgcgttctgg caggcgcggg tgctggacgt
     6541 tgaggcctgc gtggcggttc ggcactggcc gggcgacgag gtcgacttca acctcgtgct
     6601 caccgacccc ttgagcgaga tcctggcagg agaagcctgg tcgggcgtgg cgggtgagta
     6661 cgtggtgcac gtgggcgccg actcctctgc ccgatcgggc ttcgaggccg ggctgccggt
     6721 gctgcatgcg ggcgtcggtg cgtttacgag attgtggctc ggggtggtcg gcgcttcggc
     6781 tcttgctctg agcgacgagc tgacgggccc gaccgagctg ctcggccggc tggacgaggc
     6841 tctctgcctg ccgccggcca gaacgacctg ggacttttga cgattcggct ggaaatcgac
     6901 ggcatcgagg tggagctgcg ccgcaactcc cggcgtcgca cccgtatcgg catggctttc
     6961 gaccccagcg gattcgtgat catggacgcg ccccccaacg tcggcgagaa cgaagtccgg
     7021 gaggtggtcg ccgagcatca tcgctggctg cgcgtcaggc tcgacaaggc gcgcgacgcg
     7081 gttcacgaag tccgcgcgct caactatgtt gccggcgagc tcgtccacta tctcggccag
     7141 gcgtaccggc tcgaggttcg cgccgggggg gaggtgcggg tggagctcag cgcgcctgcc
     7201 cattcccagc tgccgctgtt tgccgacgtg gcgttcggta ccctgcaggt gacgacgccc
     7261 gagcagtcgc cggacggggt caaaaccgcc gtgtgcgact ggtactcggc acagggcgat
     7321 cgggtcttcg ccgaacggct ggcggggtgg tcggaacttc cttggctcaa cggaacgacg
     7381 ccgcgctggc ggcacacctt catgcgcagc cagtggggca gctgcagcgc gcggggcaag
     7441 atctcgctca acacccatct ggtcaaggcc cccgtgtctc tcgtcgacta cgtgattctt
     7501 cacgagctgt gccatctcga gcatcacgat cacggacggc gcttttacgc gttgatggag
     7561 cggcacatgc ccgactggga gcgccgccgc gccgccctgg accgcttcct gccggtgctt
     7621 ctccatgact gatctctcgt gaacggcgcc aggcccactc tccccgagct cggctggaca
     7681 ccgttcttcg ccgagcagct gtctcccgag gaggcagacc ggctcatccc cgcccgggtt
     7741 ttcggcgtgc agcggactca catcacggtg atggccgagg agggtgagct ttccatgccc
     7801 ctgggcgggc gctggtttca gctcgatccc caggaccggc ccacggtggg cgactgggtg
     7861 ctggtttctg agcagcgcga cagcgtggtg aggctgctcg aacgcagaag cctcctcaag
     7921 cgattgagcc cgggccgcac gggtgcggtg cagctcatcg cggccaacgt cgatacgctg
     7981 ttcctggtca cctcgtgcaa tcaggatttc aatccgcgcc gcctcgagcg ttacctggcg
     8041 ctggcatacg agtcgggcat cgagcccgtg gtggtgctca ccaaggcgga tctatgcgac
     8101 gatctggagc gctacgtgga tggggcccga cggatcgtcc cgggcctcga ggtggtggct
     8161 ctggacgctc gcgaccgggc acagctcgct ccccttgcgt cctggttggc gaccggccgc
     8221 acggtggcgc tgctcggctc gtcgggtgtg ggtaaatcca cgctggtcaa caccctctcc
     8281 gaggctcagg tgcagcgcac ggcgcagatc cgggaagacg acgggaaggg caggcacacc
     8341 acctcgcacc gctcgctgca cccggtgccc ggcggcgggc tgatgctgga cagcccgggt
     8401 atgcgggagc tcggcatggc ggatgcggaa ctcggcgtgc gtcagatgtt cgaggacgtg
     8461 gaagcgctcg cgcaccagtg ccgctttgcc gactgcgcgc acgactcgga acccggctgc
     8521 gcggtcaagg cggccctcga ggccggggag cttaccgagg agcggctgga gagttaccgc
     8581 aagctcagcc gggaagaagc cgtgaacgcg gaaaccattg ccgaacgtca cgcccgctcc
     8641 cgagcgacgg acaagctgca ccggcgggtc caggacgcat ctcccaagcg ccgcagctga
     8701 cgcctacctc gggagccgga gatgccttaa agtacgcgcc tcttccgccc gaggcgcgtc
     8761 atggccgacc ttctcgccga gcagctccgc ctgcagcccg tttccgtcac ccgtcgcatg
     8821 tctgcctctt tgcccggcct gcgcaggctc gctcaggaga aggggttgaa gatccaccac
     8881 ctgggagcgg ggtatcccca tcctgaagtg accgatcccc ggtcgttcat tgcccatcgg
     8941 gaagcctact tcgcccacct cgaagagcgg gaaggcctca acgaccctcg agccctgccc
     9001 gagtacctgc gggagtcgtt ctcctacacg gatacgctgg gcccggccgg cacgcgggcg
     9061 gcgtttgccc gcgtttacgg ccgggactgg ggagtcacga tggatcccgg gcgcctcata
     9121 ccgacggtgg gggcgaccgg cggcatcaat ctcgcctgct ccatgttcga gcgcccgggc
     9181 gtgccgctgg cttacatcac cgacgccccg acctacgccg gattcaacgc ccgtgcaacg
     9241 ctgtgtcagc acgccgccat ctacagcgtg cccatggatg acgaaggccc gctgccggac
     9301 gttctccgtg atcagatccg cgccgcgcgg gccgacggcc gcttcgtgcc gttctactac
     9361 acggtgccgg acgggcacaa cccggcaggg ttctcgttca gccaggcacg gcgggaagcc
     9421 attctccggg tcgtcaggga ggagggcatc ctcattctcg aggacgctcc ttacgtctac
     9481 atcaactttg cgccggccgc gaaccgcccc cggcccttcc tcgccatgga cccgcgctgc
     9541 acgatccacc tcttcaccgg atccaagatc ggctttcccg ggccgcgcgt aggctttctc
     9601 tactcggaag ccgagcttgc cattgccggc gggcggacgg cttcgctgac cgaatttgcg
     9661 ctgactgaag ccagcgccga catgctgttt cagaacccgg ccgccctgct gggcttcgaa
     9721 gcgctgctgc acgacgacga cttcagcgag cggcggtcgt tgtggccggt ggcggagacg
     9781 aagctcgacg tgtatcgcga gaaccggtcg atcatgctcg acggcctgga acgggagctc
     9841 ggcgggcatc gggatctgtt tcactggacc gaccccaagg cgggcttctt cactgttttc
     9901 acgtttctcg gcccgggaat ccgcacggac gatgccttcg tcaacgcgat ggtggccgac
     9961 catggggtgg tggtgatccc gatgtacgac ttctacccgg aggatgcccg gcgccgcgac
    10021 cccgatgccg gtttcgacca gctgcggttg tcgttctgct tcagtgaaag cctgggtgag
    10081 cagcggcgca cggatctcga ggaagcggta gccgccttct gcgcggcggc gaagaagctc
    10141 gccggggtct gagacgccga ggctccgtcg cctcagggtg tcggggccac ccttcgcggc
    10201 tcgttgcgga ccatgtccat gagcgtgttc aggtggggac gcagctcggg ctccaggtag
    10261 ctgaaattta cgccccgagg gttgaactcc cagaacaggg cccgttcgtc gctcagcagt
    10321 ccggcctgca gccgatccag ccgggaggcg ccttccgagc tcaggtcgtc ggccacccgt
    10381 tgggcccggg ccaggtcgcc ctgactgatc aggtaggcgc ccagctgcat ctgtgcccgg
    10441 cgcacgccga gcaggctctc ctcctgggat tcgtacttga tctcgtgatc cagctccaga
    10501 aagccgtcta gcatgcggtc cagggcggcg gactggttct tgtgagccag gcggatcagc
    10561 tccccgaggt cgaaagcggc aacctccaga aggaacggct gattcatggt gaaagcgccc
    10621 tgcccgtagc tctgaatgtg ctcggcggcg gcgagggcgt cctcgtcgtg gcccatggaa
    10681 accagatcct ccgccaccgc cttgtactgg ctcagcaggt ggaaggttgt ccggatgtcg
    10741 cgcgcgttca ggctggcgcg cagcaggctg ttcatgccgc gaaagctcgc ctccagcacc
    10801 agcgggtcgt cgatctgctc aatggccgtc tgccgcaggg cgatgccgac ccgaccggcc
    10861 acgtcatgca tcttcatcac ggacagccgg atgatgctga cgtactgatg catgatcttg
    10921 acctcgaccc aaagccccga ggtgtccagg tcgtccaggg cgctgggctc cagggcgacg
    10981 aagtcagggt cctcccgcac gatcccgtcg acccgggacc acccctcggg cagccggttg
    11041 cgcacggagc ggtaatgccg gaacaggtcg gacagcgcct ccaccgcggc catggcgatg
    11101 gcacggtcgc cgtgatcgat ggcgctgcgg gcgacgtcct gaagctggtc tatggttcga
    11161 agggtctccg cgcggtggcc ggcggcgacg gcatgctccg ccttgcggcg gatgcgtcgg
    11221 atgatgccga ccggcgagat gaaggcgaag acgtagccga agtagggcag cagcgcggcc
    11281 agggcaacgg taacggtaac cagggtgacg gtgttccagg tggcgttcag ctccgcctcg
    11341 cccagggcgg cgacccagat gcacaggacg gtggtgagca cgaagaaggc ctgaaccacc
    11401 acgttgatgg gctcgcggac gaacatctcg gtgatgtggt ggttgtagcg ggtcgcggcc
    11461 agctgcacga tgatggcgac caccgtgatg gagatgccga gaatcgcggc gacgacctcg
    11521 gcagaaacgt ttacggcttc cagcacggat cgggtccggt gcttgcgatg acgcaagcct
    11581 accgcaagtc gtcagaaggc gcgatgcgtg cggttacgcc tgatgtcggt tctccggaac
    11641 cggatcctgc cggaggcagg ggacggctgg aaaatgccgt ccccgatccc tatctcgata
    11701 tcagcagcga cccttcttgg cctggccggg cgggcagtgg cgtcctgcgg gatgtccccc
    11761 ggcagagccc acttccacat gcacggtgct ggcaggtgcc gtgtgcgcct ccacgtggtg
    11821 atggtcgggc ccgccatcgt tgccatcggt ggcgatggcc gtgccgatgg ctgcgcccac
    11881 cgcgctgccg atgatcgctc ccttacgatc gcccagctcg tcgccgacca cggcgccgat
    11941 ggcgcctccg gcaccgccgc ctacggcggc ctcgagcttg tcgccggccc atgccggcac
    12001 ggtcgtaacg gtggtgagaa tcagaaatcc aatggctcgg cgcatgacag gcccctgctt
    12061 tttgaaacaa ccccgggagt gtaggggggc gtcgacagca gaatcgtgac ctgggtcatg
    12121 gattctccct tgcgcgcgcg ccgtgagcct cgcggcgtgc tagtgatccc agggtgtctg
    12181 atgtatggtc gagaagttgg caaggggaca tcgtcatgac cgatcactgg cacttccaca
    12241 gcgacacctt cggcaactgc aactgcgccc agaactgcgg atgccagttc aacctcccca
    12301 gcacccatgg attctgtcag ttcgtcgagg gtggtcacat ccgcgacgga tccttcaacg
    12361 ggatgaacat ctcagggttc aactgggcgt tcatgatgat ctggccaggt gagatcgccg
    12421 agggcaacgg ccgccgccag atcgtcattg acgagcgcgc aaacgaccag cagcgagcgg
    12481 ccatcgagaa gatcgtctcc ggcgaggtgg gcatccccgg cggcaaccat ttctcagtgt
    12541 tcggcagcac ctgctccgaa tcgctggaaa ccctctacct gcccatcgag ttcgagatgg
    12601 acattgccgc tcgccgcgcg cggctggcga tccccggcct catcgaggcc agaggcgaac
    12661 ccatgatgga cgggttcagc ggcagcgagt ttcacatcgc gctggcgagg cccaagggca
    12721 gc
//
LOCUS       DBNHLM010000214         3481 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_269992, whole genome shotgun sequence.
ACCESSION   DBNHLM010000214 DBNHLM010000000
VERSION     DBNHLM010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_269992"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..526)
                     /locus_tag="AC16QF_09925"
     CDS             complement(143..526)
                     /locus_tag="AC16QF_09925"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6448915.1"
                     /translation="MADIGVCEHFYCEVLGMRVVWRPDEQNVYLTNAADNLALHQTSE
                     EEGAGRESLDHIGFGVEEADAVDAWHEFLLIHGVSIVSPPRTHRDGTRSLYCRDPGGN
                     TVQLLYEPRLAADMASKEPAGTQRQ"
     gene            556..1653
                     /locus_tag="AC16QF_09930"
     CDS             556..1653
                     /locus_tag="AC16QF_09930"
                     /inference="COORDINATES: protein motif:HMM:NF018649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIR6448916.1"
                     /translation="MSEPNAAFVIRSGYPDLRDFPALGRRNLVNVALTLWVPIPAVIS
                     AVVLFHWFPEGAVPPDPGLVWPGSADEAVSILLHHSLLTVNLLFLLFVDLQFWLIALA
                     QRSSWLIDPYWTLLPPLLAFFYFAHPFASPDVSRSALALGLLAIWSLRLTWNYFRREG
                     WRFGVREDWRYAKMRAERPLFWLEQLVVVYAVQHVLLVGLSLPFWAIAFRDVPFGALD
                     AALLLAAAAGLVIAHAADTRLDRFMRDNEARSARGERKILILEDGLWRISRHPNYFGE
                     QMFWWSVAGIGVVCGEPWVVLGTAANSAVLAGVTVMSERRMLAVPERREAFLAYRRRT
                     SVWVPWWPRHGGTAADREHAAGKRERQPSDS"
     gene            complement(1958..2614)
                     /locus_tag="AC16QF_09935"
     CDS             complement(1958..2614)
                     /locus_tag="AC16QF_09935"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR6448917.1"
                     /translation="MKRKSVVLISATGLMLVFGLATYLYTSEQADRIEALAVLPDSPL
                     ERPHSRGRGPADAKVVLVEFFDPACETCRAFEPYVKALLGEHRGRVRLVLRYAPFHKG
                     SDSMVKILEAAALQDRFWETLQVMYDTQSQWASHHNPQPEKFWEFLPRAGVDVDRIRR
                     DAQEPKFAAIIEQDLSDGRTLGVRKTPQFFVNGKPLVTFGYEQLKSMIDVEVARQYPQ
                     "
     gene            complement(2611..>3481)
                     /locus_tag="AC16QF_09940"
     CDS             complement(2611..>3481)
                     /locus_tag="AC16QF_09940"
                     /inference="COORDINATES: protein motif:HMM:NF014639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6448918.1"
                     /translation="WAPSRAGRERMAGSEARPGSLCLQHVALNVADLGACEHFYCEIL
                     GMRVVWRPDEQNVYLTNAADNLALHQTSEGEGAGRESLDHIGFAVEEADAVDAWHEFL
                     LLHGIAIVSPPRTHRDGTRSLYCKDSGGNTVQILYEPRLESDMTKAKNLSDPDWAVLF
                     VCWLLAAAATLGSLFFSEVMELPPCSLCWIQRVFMFPLTIILLIGLFPFDPRVVRYAL
                     PLAAIGGGFALYHLLLQAGLVPDSAAPCRQGVPCTEAQIQILGFVSIPMLSLLVFASV
                     TGLLLLLKRRQSP"
BASE COUNT          598 a         1125 c         1091 g          667 t
ORIGIN      
        1 gccccgcgcc ttgcatcgcg ccgaccgtgc cgcgcgggcc ctcctctggc ccacggtgac
       61 gtcgttggat gctctgcgcc ggcaggggcg tatttcgctg ctgtagagcc cctgggcaca
      121 tcgccgctag ggcccgccgt cgttactggc gctgtgttcc cgctggttct ttggaagcca
      181 tgtccgcagc gagacggggc tcgtagagaa gctgcacggt gttgccgccc ggatccctgc
      241 agtacaggct tcgcgtcccg tcgcgatgcg tgcggggagg cgagacgatc gagacgccgt
      301 ggatcaagag gaactcatgc caggcatcca cggcatccgc ttcctcgacg ccgaatccga
      361 tgtggtcgag actctcgcgc ccagccccct cttcttcaga cgtctgatgg agcgccaggt
      421 tgtcagctgc gttcgtcaga tagacgttct gctcgtccgg tcgccacacg acgcgcattc
      481 ccagaacctc gcagtagaag tgctcgcaaa cgccaatgtc tgccacgttg gaaccgattc
      541 tgtacgatct gacttgtgag cgagccgaac gccgcgttcg tgatccgatc tggctatccg
      601 gacctccgcg attttcccgc tctcggccgg cgaaacctcg tcaatgtcgc actcacgctt
      661 tgggtcccga ttccggccgt catcagcgcg gtcgtgctct tccactggtt cccggaaggc
      721 gcggtcccgc cggaccccgg gctcgtctgg cccggctctg cggacgaggc cgtttcgatt
      781 ctcctccatc actcgctgtt gactgtgaat cttctcttcc tactgttcgt tgatctccag
      841 ttctggctga tcgcactcgc gcagcgcagc agctggctga tcgatcccta ctggaccctg
      901 cttccgccac tgctcgcgtt cttctacttc gcccatccct tcgcctcgcc cgatgtctcg
      961 cggagtgcgc tcgcgctcgg cttgctcgcg atctggtcgc tgaggctcac ctggaactat
     1021 ttccggcgcg agggctggcg cttcggtgtg cgcgaggact ggcgctacgc caagatgcgc
     1081 gccgagcgcc cccttttctg gctcgagcaa ctcgtcgtgg tctacgccgt ccagcacgtg
     1141 ctcctggtag ggctgagtct tcccttctgg gctatcgcgt tccgggacgt gcccttcgga
     1201 gcgctcgatg cggcgctcct gctcgcggct gcggctggac tcgtgatcgc gcatgccgcc
     1261 gacacgcgcc tcgaccgctt catgcgggac aacgaagctc gaagtgctcg tggcgagcgc
     1321 aagatcctga ttctcgaaga cggcctttgg cgcatctcgc gccacccgaa ctactttggt
     1381 gagcagatgt tctggtggtc cgtggcgggc atcggtgtgg tctgtggaga gccatgggtt
     1441 gtgctcggaa ccgcggcgaa cagcgccgtg ctggccggcg tgacggtcat gagcgagcga
     1501 cggatgctcg ccgtgccgga gcgccgcgag gcattcctcg cctaccgccg acgcacctcg
     1561 gtatgggtcc cgtggtggcc gcggcatggt ggtaccgccg cggaccgaga acacgcggct
     1621 ggcaagcgcg aacgtcaacc ctcggattcg tgaaccgagc gcggagtggg cggggccagc
     1681 caggtcaccg gtttcaaccg gaagctgcag tcttgaaaac cgcgccgcga tcctctcggc
     1741 cgattctgtg gattacagcc tcccgctgat cgacgacgag gccgctggcc tggtagcgct
     1801 cgtgatcttt tcgccggaga ccctggagat tccgaactca cagcacagcg gggccatctt
     1861 ctcgccctcg gggagccggg caatgaatct gagacgatcg ttcgcagttc gacgcgttct
     1921 cggcggcatc gaaagtgcgg tcctggcgtt tcgcgtccta ctgcgggtac tgcctcgcca
     1981 cctccacgtc gatcatgctc ttcagctgtt cgtagccgaa agtcacgagc ggcttgccgt
     2041 tcacgaagaa ctgtggcgtc ttgcggacgc cgagcgtgcg gccgtctgag agatcctgct
     2101 cgatgatcgc agcgaacttc ggttcctgcg catcacggcg aatacgatcg acgtcgaccc
     2161 ccgcccgcgg taggaactcc cagaacttct cgggctgagg attatggtgg ctcgcccatt
     2221 gggactgcgt gtcgtacatc acctgaaggg tctcccagaa gcgatcctga agtgctgccg
     2281 cttcgaggat cttcaccatg gagtccgacc ctttgtggaa aggggcatag cgcaagacga
     2341 ggcgcacccg accccgatgc tcgccaagca gcgccttcac gtagggttcg aaggcgcggc
     2401 aggtctcgca tgccggatcg aagaactcga ccagcacgac tttggcgtcc gcaggacccc
     2461 tgccgcgcga gtgcggtcgt tctagggggg aatccggaag tacagcgagt gcctcgatgc
     2521 gatccgcttg ttcactcgta tagagatagg tcgcgagccc aaagacgagc atgagcccgg
     2581 tagccgaaat cagcacgacg gatttcctct tcacggcgac tgtctcctct tcaacaacaa
     2641 tagcaacccc gtcacgctcg cgaacacgag cagtgagagc atggggatag acacgaagcc
     2701 aaggatctgg atctgggctt cggtgcaggg cactccctga cgacagggcg cggcactgtc
     2761 ggggacgagc ccggcctgta agaggaggtg gtagagcgca aacccgccgc cgatcgccgc
     2821 cagcggcagg gcgtaacgca cgacccgggg gtcgaatgga aacaagccaa tcagcagaat
     2881 gatggtgagc ggaaacatga agaccctctg aatccagcag agagaacacg gtggcagctc
     2941 catgacttcg gagaagaaca ggctgccaag cgtggcggct gcggcgagca gccaacagac
     3001 gaacaggacg gcccagtccg gatcggaaag attcttggcc ttggtcatgt ccgactcgag
     3061 acgcggctcg tagagaatct gcacggtgtt gccgccggaa tccttgcagt acaggcttcg
     3121 cgtcccgtcg cgatgcgtgc ggggaggcga gacgatggca atgccgtgga gcaagaggaa
     3181 ctcatgccat gcatccacgg catccgcttc ctcgacggcg aagccgatgt ggtcgagact
     3241 ctcgcgccca gccccctctc cttcagacgt ctgatggagc gccaggttgt cagctgcgtt
     3301 cgtcagatag acgttctgct cgtccggtcg ccacacgacg cgcattccta ggatctcgca
     3361 gtagaagtgc tcacacgcgc caaggtctgc cacgttgagc gcaacgtgct gcaagcagag
     3421 agagcccggc ctggcctcgc taccagccat gcgttcccgg cccgcccttg aaggggccca
     3481 c
//
LOCUS       DBNHLM010000215        10151 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_808536, whole genome shotgun sequence.
ACCESSION   DBNHLM010000215 DBNHLM010000000
VERSION     DBNHLM010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10151)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10151
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_808536"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="AC16QF_09945"
     CDS             complement(<1..791)
                     /locus_tag="AC16QF_09945"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6448919.1"
                     /translation="MQQLPDPVRIPTNDIELEVFEQGAGAPVVLCHGWPEHAYSWRHQ
                     MSPLAEAGYHVIVPNQRGYGHSSRPQKVTDYDITHLAADLTGLLDHFGYDRAVFCGHD
                     WGAIAVWNLAMLHPDRVAGVINLSVPLLRRGPTDWVSHWESLLGEDFYIVHFNRRPGV
                     ADAVFEENPRRFLGNLYRTHQWQEPAAEPRSGMPLINLARANRPRGRPLMSDAELDVF
                     VRAFEHSGFSGGINWYRNFARNWKLTADVPEHVPHPAMMIYGRYD"
     gene            868..1083
                     /locus_tag="AC16QF_09950"
     CDS             868..1083
                     /locus_tag="AC16QF_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448920.1"
                     /translation="MRISQLVLKLPIPSYLNRSFYPEGFARQIAGIAIAAAGNRWPAP
                     EQLDVAAFLVHGSDDPLVPVEGGVDTH"
     gene            1258..1653
                     /locus_tag="AC16QF_09955"
     CDS             1258..1653
                     /locus_tag="AC16QF_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HIR6448921.1"
                     /translation="MNNTHSLAVGYILWIFGFLGAHRFYFGRPVSGTIYFFTLGLFLI
                     GWLIDLILMPNLEASADRRYQQGEKSYTVAWVLLTFLGFFGVHRFYQGKWMTGIIYLF
                     TVGLFGIGVIYDYWTLNEQVSALNHERAG"
     gene            1729..2493
                     /locus_tag="AC16QF_09960"
     CDS             1729..2493
                     /locus_tag="AC16QF_09960"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HIR6448922.1"
                     /translation="MDRVVDLTGAVNFRDFGGYAAADGATVRRGLLFRCGQLASLSQE
                     ALAQFAELGIDVICDLRRPDEREVDPTPVPHHVSRRVEIPMDPGSATQLRDSLRAGYV
                     DVSQRVDFMRTITRELARDHVEGYSSMFAALLEHAPGGFLVHCTAGKDRTGVGVALIL
                     LALGVPRETVIHDYMLTNDVMDFEAFILPRIRQNLGHDNIDVEGARVLSGVREEYIMA
                     ALEEMESACGSLEAYLERAIGLTRGDVDALRAHYLS"
     gene            2575..3900
                     /locus_tag="AC16QF_09965"
     CDS             2575..3900
                     /locus_tag="AC16QF_09965"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HIR6448923.1"
                     /translation="MAGTKHEAPRPTGFDTLALHAGARPDPATGARATPIYQSAAFVF
                     PDSDFAAGLFNIERAGHVYSRLSNPTNAVLEERIAALDGGVGAIATASGQAAMHLAVA
                     TLLGAGDHIVASRSLYGGTHNLLAYTLPRFGISTTFVDPRDPQAFADAVREETRLVFG
                     EILGNPGLEVMNVPAVADAAHAAGLPLMIDATFTTPYLFRPLDHGADLVVHSATKFLS
                     GHGVVIGGLIVDGGTFDWEASARFSTLSQPYDGFHGLVFAEEFGPEAFIMRARKEGLR
                     DFGACMAPMTAFQILQGLETLPLRMDRHVANARAIADFLAGHPMVTAVNHPDLAGHPD
                     HDLAKTLLPRGAGAVMGFDIKGGREAGRAFIESLQLFSHLANVGDAKSLVIHPASTTH
                     HRMDAKALEAAGIGEGLIRVSVGLEDPADLIDDLRGALKRAERAAGVST"
     gene            3897..4703
                     /locus_tag="AC16QF_09970"
     CDS             3897..4703
                     /locus_tag="AC16QF_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6448924.1"
                     /translation="MRVEVGGHCAFAGTGSVPFDARRPCVVFLHGAGFDHSVWVMPSR
                     YFARHGFAVVAPDFPAHGRSKGAPLASVPALASWLAELLDALGVAEAVVVGHSLGSLV
                     GQAFARLFPDRCLGLALLGTSAPMAVTEQLLGASRDDDHAAIDMANTWSHSPQGRLGA
                     SDNPGIWMLGAEERLLERAAPGVYHADFTACNECDLDQLPGLVSCPVLVIAGGSDSMT
                     PARSGQAVANALSARYVSLPGCGHSMLAERPNQVLNALAEFIGQLPPADH"
     gene            complement(4697..5530)
                     /locus_tag="AC16QF_09975"
     CDS             complement(4697..5530)
                     /locus_tag="AC16QF_09975"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448925.1"
                     /translation="MDRLAGRTALITGAARGLGAQIAKRFAEEGAMVIVNDLNLEAAE
                     ATAAAIGGRALACDVSDSSAVNAMFEEVASIADRLDVLVNNAGISGVEGDSNAQLRIQ
                     RAFERADAAARGEVPDLDPPILEVSDEAWGRMLAVHLNGTFYCSRAAVALLNRNTDRP
                     EGGAAIINMGSIMGTSGGAGASAYCAAKAGILGLTRSLARELAPYDIRVNAIAPGYIH
                     TDMTSPMEAMYPLLKAQTPQGRIGEPDDIAWAAVYLASLEAKFVTGQTLSPNGGWHMS
                     Q"
     gene            5678..6370
                     /locus_tag="AC16QF_09980"
     CDS             5678..6370
                     /locus_tag="AC16QF_09980"
                     /inference="COORDINATES: protein motif:HMM:NF018949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0090471 - 9,15,9'-tri-cis-zeta-carotene
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0016120 - carotene biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HIR6448926.1"
                     /translation="MLQTLLAGLIFVGAHLGISSTSLRPRLVAAVGERAYQGIFSLVA
                     LVTISLLIWTYGEAPRYEYLWLPDPRLYWVTKVVMVVAFISMIGGFMVKNPTAVGMEG
                     ILKDPGQREHAATGMLRITRHPFQWSVVLWSASHIVANGDTVSVVFFAAFGVLSLLGT
                     LAIDRKKAAALGDDWEHFAAVTSNLPFAAVLSGRNRLVLKELVGPGAAGLVVYALVFW
                     GHQWVSGGVRLL"
     gene            complement(6367..6966)
                     /locus_tag="AC16QF_09985"
     CDS             complement(6367..6966)
                     /locus_tag="AC16QF_09985"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6448927.1"
                     /translation="MKIEIPGPAGPIEAELDDANDGSGSFAVLCHPHPQYGGTMHDAV
                     LSVLGRALAGAGVGCLRFNFRGVGGSAGAHDGKGGEVEDLLAVERWLRQEHSPAHLIL
                     GGYSFGAAVAWQALTSLAPPDQMILIAPPVSFMPSTRRPVGFRLDVFAGDADQFVDAA
                     ALNVLGDAGIHIIPGADHFFGGKWGDLAAGIEQVLQPAG"
     gene            7164..8075
                     /locus_tag="AC16QF_09990"
     CDS             7164..8075
                     /locus_tag="AC16QF_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6448928.1"
                     /translation="MIEADGLTRRYGDFVAVRNVSFSIEHGEIVGLLGHNGAGKTTIM
                     KMLTGYLEPSGGSVRVDGADIEQDRLRVQRELGYLPENLPLYPEMTVSDYLSYAATLR
                     GVDPRLAVARAVEETELEEKALDPISTLSRGFKQRVGVAQAILHEPRFVILDEPTNGL
                     DPSQTQHMRELIQRLAATATVVLSTHIMQEVDAICDRALILRDGRLVLDERLADLKRS
                     DQLRLRCAGGGPVADLLAGVSGVSRAESVAEGAWQLTVDGPLDAAAERLARALVDAGI
                     ALHELGPVQRDLETVFREVNEGVSDAA"
     gene            8065..>10151
                     /locus_tag="AC16QF_09995"
     CDS             8065..>10151
                     /locus_tag="AC16QF_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024141.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7088 domain-containing protein"
                     /protein_id="HIR6448929.1"
                     /translation="MRPDVVKRIAGKELTLFFASPVGYLFLGAFLAFTLFVFFWVEAF
                     FARNVADVRPMFEWMPVLLVFLSAALTMRMWSEERRTGTLEFVATVPVSGWEFVVGKF
                     LACWVLLAIALLLTLPLPISVSLIADLDWGPVWAGYLAAMLLGGAYLAIGLFVSARTD
                     SQIVSLILASFACGAFYLLGSPVLTDLVGGGLADILRSVGSGSRFESITRGVLDLRDL
                     YYYASLLAVFLAANVHALEVQRWAADGGAGRHRRWLLGTGLLVANLLLANVWLAQLTG
                     LRIDVTEGRIYSVSDATRAYIGQLREPLLIRGYFSNRTHPLLAPLVPRMRDLLNEFEV
                     AGAGNIRVEIVDPADDPAAEDEANSKYGIRPVPFQVADRYESSLVNSYFDVLLSYGDE
                     YEVLGFRDLIEVKVVGEADLDVQLRNPEFDLTRAIKKVLYGFQGGGSLFANIQDPVRF
                     VGYVSSADRLPPALADYRAVLVEALDALARESGGRFTSEFIDPDAGDGSVAAQIAVDF
                     GFQPMAASLFDSNVFYFYLTLGDANTLIQLPLPEAFSVEATRRVLEEGLKRFASGLLK
                     TVALAAPEAPPYMGMGQPPQGNQYEQLKDVLRSDFEVETTDLAEGVVPDSADLLMIVD
                     PEGFNRRQLFAMDQFLMKGGTVVVATAPFSASLSQQSLMAAPRTSGLEAWLEHMGVTL
                     EPKLVMDAQNAAFP"
BASE COUNT         1531 a         3296 c         3444 g         1880 t
ORIGIN      
        1 atgtcgtagc ggccatagat catcatcgcc gggtgcggaa cgtgctcggg cacgtcagcg
       61 gtcagcttcc agttgcgcgc gaagttccgg taccagttga tgccgccgga gaacccggag
      121 tgttcgaagg cacgtacgaa aacgtccagc tcggcgtcgc tcatcagcgg cctgccgcgc
      181 ggccggtttg cccgtgcgag gttgatcagc ggcatgccgg atcggggctc ggctgctggc
      241 tcctgccatt gatgggtacg gtacaggttg ccgaggaagc gtcgcgggtt ttcctcgaag
      301 accgcgtctg ccacgccggg ccggcggttg aagtgaacga tgtagaaatc ttcgccgagg
      361 aggctttccc agtgcgagac ccagtccgta ggcccccggc gcagcagagg tacgctcaag
      421 ttgatcaccc cggccaccct gtccgggtgc agcatggcca ggttccatac cgcgatggcg
      481 ccccagtcat ggccgcagaa taccgcccgg tcgtacccga aatgatcgag caggccggtg
      541 aggtctgcgg cgagatgggt gatgtcgtag tcggtcacct tctgcggtcg gctcgagtgc
      601 ccgtaaccgc gctggttggg cacgatcacg tggtagccgg cctcagccag ggggctcatc
      661 tggtgtcgcc aggagtaggc gtgctccggc cacccgtggc acagcaccac tggcgcgccc
      721 gcgccttgct cgaaaacctc gagctcgatg tcgttggtgg ggatccggac tgggtcgggc
      781 agctgctgca acgggacgtt ccttgtcggg tggagaaaac cctactccga gagcgtcccg
      841 gcgtgcaatc cgatgttgtg aggcagcttg cgaatctcgc agctggttct gaaattgccg
      901 atcccctctt acctaaaccg cagcttctac cccgaaggtt ttgcgcgcca gatcgccggc
      961 atcgccatcg ccgcggccgg taaccggtgg ccggcgccgg agcagctcga cgtcgcggct
     1021 ttcctcgttc acggcagcga cgaccccctc gtccccgtgg agggcggcgt ggatacccac
     1081 taggcgctcg agcggtccag actcctggtg ctcgagggca tcgggcacga tctgccggag
     1141 ccggtcgggc ccgaggtcat ccggggcatt gcggagctca cggcacgtca ccgttgacgg
     1201 gcggcgccgg ttcggggcaa cgtgaagcat ctttcgcctg atctgctgga ggcccgcatg
     1261 aacaacacgc acagccttgc ggtcggatac atcctctgga tattcgggtt cctgggcgcg
     1321 caccgcttct atttcggccg gcccgtaagc gggaccatct acttcttcac cctgggcctg
     1381 ttcctcatcg gctggctcat cgacctgatc ctcatgccca acctggaggc gtccgccgac
     1441 cggcgctatc agcagggcga gaagagctac acggtggcct gggtgctgct cacgtttctg
     1501 ggtttcttcg gcgtccaccg cttttatcag ggcaagtgga tgaccgggat catctatctc
     1561 tttaccgtgg gcctgttcgg gatcggcgtg atctacgact actggacgct caacgagcag
     1621 gtgagcgcgc tgaatcacga acgggcgggc tgagcggcgg caggctgcca tctcggcttg
     1681 cgcttctggt aagctcgccg cctcttcacg ctgccacggg aaccttgcat ggatagggtc
     1741 gtcgacctca cgggcgcggt gaactttcgc gatttcggtg gctacgccgc cgctgacggc
     1801 gcgacggttc gtcgtgggtt gctgttccgt tgcggtcagc ttgccagcct gagccaggaa
     1861 gcgctggcgc agttcgcgga gctgggcatc gacgtgatct gcgatctcag gcggcccgac
     1921 gagcgggagg tggatccgac gcccgtgccg catcacgtct cccgccgggt cgagattccc
     1981 atggacccgg gcagcgcgac ccagctgcgg gactcgctgc gggctggtta cgtggacgtg
     2041 agccaacggg tcgacttcat gcgcaccatc acgcgggagc tcgcccgcga tcacgtcgag
     2101 ggttactcgt cgatgtttgc cgcgctgctc gagcatgccc cgggcgggtt cctcgtgcac
     2161 tgcaccgccg gaaaggaccg cacgggggtc ggggtggcgc tgatcctgct ggcgctcggc
     2221 gtgccccggg aaaccgtcat ccacgactac atgctcacca acgatgtgat ggatttcgag
     2281 gcgttcatac tgccgcgcat ccgccagaat ctgggtcacg acaacatcga cgtcgaaggg
     2341 gcccgggtgc tgtcgggggt tcgcgaagag tacatcatgg ccgctctgga ggagatggag
     2401 agcgcctgcg gaagcctgga agcctacctg gagcgcgcca tcgggctcac ccgcggtgat
     2461 gtcgacgcgc tgcgcgcgca ctacctgagc tagggtccgg gcccggagaa cggattgcca
     2521 ctgcccgtgg ggctcggtta gcctcgtcgc cctcctatgc ggaaggcgcc catcatggca
     2581 ggcacaaaac acgaggctcc ccgacctacc ggattcgata ctctggcgct gcatgccggc
     2641 gcgcgaccgg atcctgcaac gggcgcccgg gcgacgccca tctatcagag cgccgctttc
     2701 gtcttccccg actcggactt cgccgccggg cttttcaaca tcgagcgcgc cgggcacgtt
     2761 tactcgaggc tttcgaatcc caccaatgcc gtgctggagg agcgcatcgc tgcgctggac
     2821 ggtggcgtag gcgccatcgc cacggccagc ggccaggccg ccatgcatct tgcggtggcc
     2881 acgctgctgg gcgcaggaga tcacatcgtt gcttcgcgat ctctctatgg cggcacccac
     2941 aatcttctgg cgtataccct gccgcgattc ggtatcagca ccaccttcgt ggatcctcgc
     3001 gacccccagg cgtttgctga cgccgtgcgg gaagagaccc gcctggtgtt cggtgagatc
     3061 ctcggcaacc cggggctcga ggtgatgaac gtccccgccg ttgccgacgc ggcgcacgca
     3121 gcggggctcc cgctgatgat cgacgccacg ttcaccaccc cgtacctgtt ccgccctctc
     3181 gatcacggcg cggacctggt tgtgcattcc gcgaccaagt tcttaagcgg ccacggagtg
     3241 gttatcggcg gcctgatcgt cgacggcgga accttcgact gggaggcctc ggcgaggttt
     3301 tcaaccctgt cccaacccta cgacggtttc cacgggctgg tatttgccga agagttcggc
     3361 ccggaagcgt tcatcatgcg cgcccggaag gagggtctga gagatttcgg tgcgtgcatg
     3421 gcgccgatga cggcctttca gatcctccag ggcctcgaaa cgctgccgct gcgcatggac
     3481 cggcacgtgg ccaatgctcg agcaattgcg gattttctag ccgggcatcc catggtgacc
     3541 gccgtgaacc atcccgatct tgccgggcac cccgatcacg acctggcgaa gacgctgctg
     3601 ccgcgcggag cgggtgcggt gatgggattc gacatcaagg gcgggcggga agccgggcgc
     3661 gcgttcatcg aaagcctgca gctcttctcg cacctggcga acgttgggga cgcgaagtcg
     3721 ctggtcatcc atccggccag cacgacccac catcgcatgg acgcaaaggc cctcgaggcg
     3781 gccgggatcg gcgaagggct cattcgggta tcggtcgggc tcgaagatcc ggccgacctg
     3841 atcgacgatc tgcggggggc gttgaagcgg gccgagcggg cggccggagt gtccacatga
     3901 gggtggaagt cggcggtcac tgcgccttcg cgggcacggg cagcgtgccg ttcgacgcac
     3961 ggcggccctg cgtggtgttt cttcacgggg cgggcttcga tcacagcgtc tgggtcatgc
     4021 ccagccgtta cttcgcgcga cacggtttcg cggtggtggc gccggacttt cccgcccacg
     4081 ggcgatcgaa gggggcgccg ttggcttctg taccagccct tgcatcctgg cttgccgagc
     4141 tgctcgacgc gctgggcgtg gcggaggccg tcgtcgtcgg tcacagcctg ggctccctgg
     4201 ttgggcaggc ctttgcccgg ctctttcccg accgctgcct ggggctcgcg ctgctgggca
     4261 cgtccgcgcc catggcggtt accgagcagc tgctgggagc gtcccgggac gacgatcacg
     4321 cagccatcga catggcgaat acctggagtc acagtcctca gggtcggctc ggggccagcg
     4381 acaacccagg catctggatg ctgggcgccg aggagcgtct gctggagcgc gcggctcccg
     4441 gcgtctatca cgccgacttc accgcctgca acgagtgcga tctggatcag ctgcccgggc
     4501 ttgtgagctg ccccgtgctg gtgatcgccg gaggttcaga cagcatgacg cccgcgcgct
     4561 cggggcaggc ggtggccaat gccctttcag cgcgctacgt aagccttcct ggctgcggtc
     4621 actccatgct cgccgagcgg cccaaccagg tgctcaacgc cctggcggag ttcatcggcc
     4681 agctgccccc cgccgatcac tgactcatgt gccacccgcc gttcggcgac agggtctgac
     4741 cggtgacgaa cttcgcttcg aggcttgcca ggtagacggc ggcccaggcg atgtcgtctg
     4801 gctcgccgat gcggccctgg ggcgtctgag ccttgagcag cgggtacatg gcttccattg
     4861 ggctcgtcat gtccgtgtgt atgtatcccg gcgcgatggc gttcaccctg atgtcgtaag
     4921 gcgcaagctc ccgggccagg gaccgggtga gcccgaggat cccggccttg gcggcgcagt
     4981 aggcggaagc cccggcgcca cccgaggtgc ccatgatgct gcccatgttg atgatcgcgg
     5041 cgccgccctc cggccggtct gtgttgcggt tgagcaacgc cacggccgcg cggctgcagt
     5101 agaacgtgcc gttgaggtgg acggcgagca tgcgccccca ggcttcgtcg ctcacttcca
     5161 ggatcggcgg atcgaggtcg ggcacctcac cacgcgcggc ggcgtccgcc cgctcgaatg
     5221 cccgctggat gcgcagctga gcgttgctgt cgccctcgac gccgctgatg ccggcgttgt
     5281 tgacgagaac gtcgaggcgg tcagcaatgg aggcgacctc ctcgaacatc gcgttgacgg
     5341 cggacgagtc gctcacgtca caggccaacg cccgcccccc gatggcggca gccgtggctt
     5401 cggccgcttc gaggttgaga tcgttcacga tcaccatggc gccttcctcc gcgaaccgct
     5461 tggcgatctg cgcgccgaga cccctggcgg cgcccgtgat cagcgccgtc ctgcctgcca
     5521 gtctgtccaa tgtcttccct ccttcccggg tgcccgacga tcgaccttgt catcaagtgt
     5581 ggccgaacac ctcggcgaag cgcaacgagc aggcagcgcg aataagccgt ctttcgagta
     5641 gtatccgtcc gaagctggtc aggaagaccc agagaccatg ttgcagacgc tgttggccgg
     5701 cctgattttc gtgggcgccc atctgggtat cagcagcacg tcgctccggc cgcggctggt
     5761 ggccgcggtg ggcgagcgtg cctaccaggg aatattctct ctcgttgccc tcgttacgat
     5821 ttctctgctg atctggacct acggggaagc tccccgatac gagtacctgt ggttgccgga
     5881 cccgcgcctg tactgggtga ccaaggtggt catggtcgtg gcgttcatct ccatgatcgg
     5941 cgggttcatg gtcaagaatc ccacggccgt gggcatggag ggtatcctga aggatcccgg
     6001 gcagcgcgag cacgcggcca cgggcatgct tcgcatcacc cgccatccgt ttcagtggag
     6061 cgtcgtcctg tggtcggcat cgcacatcgt cgcgaacgga gataccgtgt cggtggtgtt
     6121 cttcgccgcc ttcggcgtcc tgagcctgct ggggaccctg gccatcgacc gcaagaaggc
     6181 ggccgcgctc ggtgacgact gggagcactt tgctgcggtc acctcgaacc tgcctttcgc
     6241 cgcggtgctg tccggccgca accggctcgt gctcaaggag ctggtggggc ccggtgccgc
     6301 ggggctggtg gtctacgccc tggtgttctg gggccaccag tgggtttcgg ggggcgtgcg
     6361 cctgctctag ccggccggct gcagcacctg ttcgatcccg gcggcgagat cgccccactt
     6421 gccaccgaag aagtgatctg cgccggggat gatgtggatc ccggcatctc cgagcacgtt
     6481 cagcgcggcg gcgtcgacga actggtcggc gtcgccggcg aaaacgtcca gcctgaagcc
     6541 gacgggccgg cgggttgaag gcatgaagct cacgggaggg gcgatgagaa tcatctggtc
     6601 gggtggcgcg aggctggtga gcgcctgcca ggcgaccgcg gcaccgaagg aatagccgcc
     6661 caggatcaga tgagcggggc tgtgctcctg gcgcagccag cgctcgacgg caagcagatc
     6721 ctcgacctcg ccgcccttgc cgtcgtgggc gcccgcgctc ccgcctacgc cgcggaaatt
     6781 gaagcgcagg cagcctacgc cggcgcccgc gagcgcgcgg cccaggaccg agagaacggc
     6841 gtcgtgcatg gtgccgccgt actgcgggtg cgggtgacag agcacggcga acgagccgga
     6901 tccgtcgttg gcatcgtcga gttcggcctc gatgggaccg gccggtccgg gaatctcgat
     6961 cttcattggc agcgtccagc gggcgttcgg atcacggtac cagcggccag gtgccggcgt
     7021 cagcccttga atttttcggc catttcccta tttttcgcac tcccgccagt tccttggacg
     7081 ggcgcgcggg cggggcatcg gggctgaacc cggcctcgaa atcggaatcg aaaaaagcgg
     7141 caacgggttg gcaggttgat ctcatgatag aagcagacgg attgacgcgg cggtacggcg
     7201 acttcgtcgc ggtgcggaac gtgtccttct ccatcgagca cggtgagatc gttggcctgc
     7261 tcgggcacaa cggcgcgggc aagacgacga tcatgaaaat gctcacgggc tatctcgagc
     7321 ccagcggcgg gtcggtacgc gtggacggcg ccgacatcga gcaggacagg cttcgcgttc
     7381 agcgcgagct tggctacctg cccgagaacc tgccgctcta cccggagatg acggtcagcg
     7441 actatctgtc ctacgcggcc accctgcggg gcgtggatcc gcgcctcgcc gtcgcccggg
     7501 ccgtggagga gaccgagctc gaagaaaagg ccctggaccc catcagcacg ctttcgcgcg
     7561 gcttcaagca gcgcgtcggg gtggctcagg ccattcttca cgaaccgagg ttcgtgattc
     7621 tcgatgagcc caccaacggg ctcgatccca gccagaccca gcacatgcgc gagctgatcc
     7681 agcggctggc ggccaccgcg acggtggtgc tctcgacgca catcatgcag gaggtggacg
     7741 cgatctgtga tcgagcgctg atcctgcgcg acggccgcct ggtcctcgat gagcgcctgg
     7801 ccgacttgaa gcgcagcgat cagctgcgcc tgcgttgcgc cggcggcggc cctgtggccg
     7861 atctgctcgc cggcgtttcc ggggtgagcc gcgcggaatc ggtggcggaa ggggcctggc
     7921 agctgaccgt ggacgggccg ctggatgccg cggcggagcg tctggcccgg gcgctggtgg
     7981 acgccggcat cgcgcttcac gagctcggcc cggtgcagcg ggacttggag accgtgttcc
     8041 gggaggtcaa cgagggggtg agcgatgcgg cctgatgtcg tcaagcggat cgccggcaag
     8101 gagctgacgc tcttctttgc ctcgccggtc gggtacctgt ttctcggtgc tttcctggcc
     8161 ttcacgctgt tcgtgttctt ctgggtggag gctttcttcg cccgcaacgt cgccgacgtg
     8221 cggccgatgt tcgagtggat gcccgtgctg ctggttttcc tttctgcggc gctgacgatg
     8281 cgcatgtgga gcgaagagcg ccggacgggg acgctggagt tcgtcgccac ggtgcccgtg
     8341 agcggctggg agttcgtggt ggggaagttc ctggcgtgct gggtcctgct tgccatcgcc
     8401 ctgctgctga ccctgcctct gcccatatcg gtctcgttga ttgccgatct cgactggggc
     8461 ccggtctggg ccggctacct ggctgccatg ctgctcggcg gcgcctatct ggccatcggg
     8521 ctcttcgtca gcgcccgcac cgacagccag atcgtcagcc tgatcctggc ctcgtttgcc
     8581 tgtggtgcgt tctatctgct cggcagcccg gtgctcacgg atctcgtcgg cggcgggctg
     8641 gccgacatcc tgcgcagcgt cggcagcggc tcgcgcttcg agtccatcac ccgcggcgtc
     8701 ctggatctcc gggatctcta ctactacgcc tcgctgctgg ccgttttcct cgccgcgaac
     8761 gtgcatgccc tggaggtgca gcgctgggct gcggacggcg gcgccggccg acaccgccgt
     8821 tggctgctcg gaaccgggct gctggtcgcc aacctgctgc tggcaaacgt ctggctggcg
     8881 cagctgaccg gtttgcgcat cgacgtcacc gaaggccgca tctactcggt ctcggacgcg
     8941 acccgggcgt acatcgggca gctgcgggaa ccgctgctga ttcgcggtta cttcagcaat
     9001 cgaacgcatc cgctgctcgc gcccctggtt ccccggatgc gcgacctgct caacgagttt
     9061 gaagtggccg gcgccggcaa tatccgtgtg gagatcgtcg atccggccga cgaccccgcc
     9121 gcggaggacg aggccaacag caaatacgga atccggccgg tgccgttcca ggtcgcggac
     9181 cgttacgaat cgagcctggt gaattcctat ttcgacgtcc tgctgagcta cggcgacgag
     9241 tacgaggtgc tcggcttccg cgatctcatc gaggtcaagg tggtgggcga ggccgacctc
     9301 gacgttcagc ttagaaaccc cgagttcgat ctgacccgcg ccatcaagaa ggtcctttac
     9361 ggctttcagg gcggcggcag cctcttcgcc aacattcagg atcccgtgcg cttcgtaggc
     9421 tacgtctcca gcgccgaccg gctgccaccc gcattggccg actaccgggc cgttctcgtg
     9481 gaggccctgg atgcgcttgc ccgggagtcc ggcggccggt tcacttcgga gttcatcgac
     9541 ccggacgctg gtgatggctc ggtggccgcg cagattgccg tggacttcgg tttccagccc
     9601 atggccgcca gccttttcga ttccaacgtg ttctatttct acctgaccct gggcgacgca
     9661 aacacgctca tccagcttcc gctgccggag gcgttctcgg tcgaagcgac ccggcgggtg
     9721 ctcgaggagg ggctcaagcg ctttgccagc gggttgctga agaccgtggc cctggcggct
     9781 cccgaagcgc cgccgtacat gggtatgggc cagccacccc aaggcaacca gtacgagcag
     9841 ctcaaggacg tcctgcgcag cgatttcgaa gtggagacca cggacctggc cgagggtgtc
     9901 gtgcccgaca gcgccgatct cctgatgatc gtggatcctg aagggttcaa ccggcgacag
     9961 ctctttgcga tggatcagtt tctgatgaaa ggcggcacgg tggtggtcgc cacggcgccc
    10021 ttttccgcgt ctctgtccca gcagagcctg atggcggcgc cgcgcacgtc gggcctggaa
    10081 gcgtggctcg agcacatggg ggtgacgctc gagcccaagc tggtaatgga tgctcagaac
    10141 gcggcgttcc c
//
LOCUS       DBNHLM010000216        10201 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1588070, whole genome shotgun sequence.
ACCESSION   DBNHLM010000216 DBNHLM010000000
VERSION     DBNHLM010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10201)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10201)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10201
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1588070"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="AC16QF_10000"
     CDS             complement(<1..276)
                     /locus_tag="AC16QF_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HIR6448930.1"
                     /translation="MAENTLKLIKDNDVKWVDLRFTDPKGKEQHTTIPAHRLDADALN
                     DGFMFDGSSISGWKSINESDMILMPDDSTPVIDPFRDDTTLNLRCDII"
     gene            complement(476..760)
                     /locus_tag="AC16QF_10005"
     CDS             complement(476..760)
                     /locus_tag="AC16QF_10005"
                     /inference="COORDINATES: protein motif:HMM:NF013379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR6448931.1"
                     /translation="MSELETLDARGLLCPLPVIRTQEKVKGLPPGTVVEIVATDPGVM
                     QDIPAWCRVHGHELISMTEGEEEAAAEGKADADRLIRGGREIRVRIKVRQ"
     gene            complement(747..1829)
                     /locus_tag="AC16QF_10010"
     CDS             complement(747..1829)
                     /locus_tag="AC16QF_10010"
                     /inference="COORDINATES: protein motif:HMM:NF014610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448932.1"
                     /translation="MPRPKRWRIMAEQRRAVALISGGLDSMLAAKVVMEQGVHVEGVN
                     FFTGFCVEGHTHAIREKDRNKPKRNNALWVAEQLGIRLHIIDVIDEYKDVLLNPKHGY
                     GAHLNPCLDCKVFMVRKALAWSFMEENGFDFIVTGEVIGQRPKSQRKETMPIIARESG
                     ADDRLLRPLCAKHLPPTLPEREGWVDREALYDFHGRNRKPQFALARRFGFEDWSQPAG
                     GCCFLTNAQYSAKLTDLWQAKGSRDYELDDIMLLKVGRHLRPRPHFKLIIGRELGENR
                     FLQGYRHQFTSLTAISHKGPLALIDGDAGADDIELAARILARYGQGRDAARVEVIAAE
                     PGGAETRLSVAPLAEADIGTDWHVGA"
     gene            1930..3732
                     /gene="typA"
                     /locus_tag="AC16QF_10015"
     CDS             1930..3732
                     /gene="typA"
                     /locus_tag="AC16QF_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR6448933.1"
                     /translation="MNTDHLRNVAIIAHVDHGKTTLVDRLLQQTGSLDRDQPERVMDS
                     NELERERGITILSKNTAIEYEGYRINIVDTPGHADFGGEVERILSMVDSVLLLVDAVE
                     GPMPQTRFVTEKAFAAGLSPIVVVNKVDRPGADPYRVIDQVFDLFDSLGASDEQLDFP
                     VVYTSALLGQAGPEPDALVDDMTPLLQSIIAHVPPPSVDADGPLQMQISSLDYSSYVG
                     VMGVGRIRRGRLARNTPVVVVDRNGRTRKARVLAVLGYHGLERIEREEAGAGDIVCVT
                     GVEEIGISDTICAPEHVEALPPLTVDEPTLTMMFCVNTSPFAGRDGSFLTSRQLRERL
                     LREASHNVALAVAETDDPDRFEVSGRGELHLSVLIETMRREGYEVAVSRPRVIMKEED
                     GRLVEPYETITIDVDDEHQGKVMESLGERRGELQEMHPDGHGRVRLRYRIPTRGLMGY
                     RPQFLSMTSGTGIMTYASAGYGPRFDGEIGRRTNGVLVSNAKGKAVAYALFNLQNRGR
                     LMIGPNDEVYEGMVVGVHSRDNDLTVNPMKEKKLTNIRASGSDENILLTTPIRLGLEQ
                     ALEFIDDDELVEITPTAIRVRKQWLKEHERKRAG"
     gene            3761..4198
                     /gene="dtd"
                     /locus_tag="AC16QF_10020"
     CDS             3761..4198
                     /gene="dtd"
                     /locus_tag="AC16QF_10020"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003103447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR6448934.1"
                     /translation="MRVVVQRVSSASVTVDDEAVASISTGLLVFLGVFPDDGDAQLHW
                     MADKVLGLRVFPGDDKPMNQSVVDVAGEVLVVSQFTLAADTSRGKRPGFSTAAPPDLA
                     ETIYERFVARLAALYPRVASGRFGADMKVALVNDGPVTFLLDR"
     gene            complement(4258..5037)
                     /gene="tatC"
                     /locus_tag="AC16QF_10025"
     CDS             complement(4258..5037)
                     /gene="tatC"
                     /locus_tag="AC16QF_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR6448935.1"
                     /translation="MAGAEDSDNRLSHDEQVDADEQPFLDHVIELRSRILRGLLVVLL
                     LFFPTYYYANDLYELVAAPLLAHLPGGGKMIATEVASPFLTPFKLSVFTAVFIGMPFL
                     LHQMWAFISPGLYLHEKKFAVPVLLSSMVLFYGGVAFAYFLVFPLVFQFMAAVTPEGV
                     TMMTDINRYLDFVLKIFFAFGFAFEIPIATFMLAWSGIATADGMANKRPYVIVGCFIV
                     GMLLTPPDVISQLLLALPTWVLFEVGIVFARLAERRSNEPG"
     gene            complement(5024..5362)
                     /gene="tatB"
                     /locus_tag="AC16QF_10030"
     CDS             complement(5024..5362)
                     /gene="tatB"
                     /locus_tag="AC16QF_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016395362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - transmembrane protein
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HIR6448936.1"
                     /translation="MFDIGFPELVLIAVVALLVIGPERLPEALRTLGLWLGRMRRSFT
                     AVKTEIEKEIGMDDVRRQLHNEAIMEEMKRIEGEVRGTADSPAGDAQTEAAGNPPSNE
                     TSATADNGGR"
     gene            complement(5365..5598)
                     /gene="tatA"
                     /locus_tag="AC16QF_10035"
     CDS             complement(5365..5598)
                     /gene="tatA"
                     /locus_tag="AC16QF_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIR6448937.1"
                     /translation="MFGLGMTELIVILIIVVLIFGPKRPKTIGSDLGNAIKGFRKAVT
                     EEEAKAAPAEEPRVIEGEVNAAQKTETKQDSSV"
     gene            complement(5676..6023)
                     /locus_tag="AC16QF_10040"
     CDS             complement(5676..6023)
                     /locus_tag="AC16QF_10040"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HIR6448938.1"
                     /translation="MTDSKDIVEDLIEVLESRRSADPGSSYVASLYADGLNRILEKVG
                     EEATEVVLAAKDATSSDRDDALVHEVADLWFHTLVMLVHLNQDPRWVFETLRERFGIS
                     GHEEKSSRNAGGP"
     gene            complement(6027..7691)
                     /gene="ubiB"
                     /locus_tag="AC16QF_10045"
     CDS             complement(6027..7691)
                     /gene="ubiB"
                     /locus_tag="AC16QF_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HIR6448939.1"
                     /translation="MSALRRLFRVLWIVSRYRLDTLIDADQEGAALPRRYAWLLRASP
                     ARLLPVGDEPRGRRLLLALEQLGPVFVKFGQLLSTRRDLIPLDIADELSALQDRVPPF
                     PGEQAVRIVEDALGQPISEAFRSFEIEPMASASVAQVHAAILPDGKSVVVKVVRPDIA
                     DVIRADLELLRAAAEILENASEEARRLHLKQVVKDYEQTILGELDMQKEADNTARLRS
                     NFAESELLYVPRVYWRWCRSNILVIERIHGIPISDVAELKARGTNMRRLAEKGVETFF
                     KQVFVDNFFHADMHPGNIFVDVTDPEDPRYIAVDCAIIGSLNQEDQDYLARNLLAFFN
                     RDYAEVARLHLESGWVPDETDPEEFERVIREVCEPIFAKPLNEISFGQFLVELFETAS
                     AFHMEIQPQLVLLQKTLLYIEGLGRELYPELDLWETAKPFMESWMAERVGPAAALREL
                     AERAPEILAELPRLPGMVVTASRQIRHLERMVDRQNRELHELEARVGRLARGVRGRRV
                     AGAALLVLASVLLWGPIAESLSGSGDLGTVAGLASAAIASLLLLKA"
     gene            complement(7688..8326)
                     /locus_tag="AC16QF_10050"
     CDS             complement(7688..8326)
                     /locus_tag="AC16QF_10050"
                     /inference="COORDINATES: protein motif:HMM:NF014132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HIR6448940.1"
                     /translation="MSTADVFESALVELVNRAANATLELDPKAASRLAAAEGHRARLI
                     AALPGGREQSFTLSVVGGRMAFTPHAEAEPNVVVRGSVPALLSMLAGGRSAGPVTIEG
                     DVSVLEELAAIFKGFAPDLAEPLSKLVGETSADGILNAAEAGLALLKSAAEATGESLR
                     RGVGERFATRTRVNVLLDRLEDARERSDRLAARIGNLESARGSHQRPEPPTP"
     gene            complement(8323..9066)
                     /locus_tag="AC16QF_10055"
     CDS             complement(8323..9066)
                     /locus_tag="AC16QF_10055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008096122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6448941.1"
                     /translation="MSDQNSVDFGSRKVSPDEKTRLVGGVFASVAERYDVMNDLISLG
                     THRLFKRMTVELSGVREGMRVLDLAGGTGDLSALFSGAVGPSGVVVLADINARMMDVG
                     RNRLLDQGCANVSFCQAAAEALPFADDSFDCVSIGFGLRNFTDKRVALEELNRVIAPG
                     GVLLILEFSRPENAFLEGAYGTFQSLWPGLGKLVAGDARSYQYLIESIRNHPPQKALK
                     LMLEDAGFAAVEYHNLLGGIAAIHRGVKP"
     gene            complement(9063..9476)
                     /locus_tag="AC16QF_10060"
     CDS             complement(9063..9476)
                     /locus_tag="AC16QF_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019412278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase family protein"
                     /protein_id="HIR6448942.1"
                     /translation="MQRPKQITYRRQSKVLVLSFEDGDAEIPAELLRVYSPSAEVRGH
                     SAAERKLQTGKKHVGIKSLEPVGNYAIRITFDDGHDTGLYGWDLLRDLAENADGYWKS
                     YLDELKAANASRLPTIPVGHWTPASQPAVPPEQDQ"
     gene            complement(9493..>10201)
                     /gene="hslU"
                     /locus_tag="AC16QF_10065"
     CDS             complement(9493..>10201)
                     /gene="hslU"
                     /locus_tag="AC16QF_10065"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010213775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HIR6448943.1"
                     /translation="GRKRSVRLKIKDALRKLKEEEAAKLVNEDEIKARAVETVEQMGI
                     VFLDELDKVAKRSESAGADVSREGVQRDLLPLIEGCTVSTKYGMVKTDHILFIASGAF
                     HLAKPSDLIPELQGRLPIRVELAALSAGDFKRILVEPNASLTEQYQALLGIDGLSLEF
                     TDDGIERIAELAWQVNESTENIGARRLHTVLERLLENVSFTATESAESTVVIDAVYVD
                     AQLSELAQDRDLSRYIL"
BASE COUNT         1779 a         3238 c         3266 g         1918 t
ORIGIN      
        1 gatgatgtcg cagcgcaggt tcagggtcgt gtcgtcccgg aacgggtcga tgacgggagt
       61 ggagtcgtcg ggcatgagga tcatgtccga ctcgttgatg gacttccagc cggagatgga
      121 cgagccgtcg aacatgaaac cgtcattgag cgcgtcggcg tcgagccggt gcgccggtat
      181 ggtggtgtgc tgttcctttc ccttcgggtc ggtgaagcgc agatccaccc acttgacgtc
      241 gttgtccttg atgagtttca gagtattttc ggccattgtg tgtacgtgct cctttctagg
      301 gtgtgcgtag taatgtcttt tgcggtatcc gcgccgtgcg accgcggaat gcagtaagcg
      361 tgccacggcc attttcttga tatcagaggt tagtgtcggc tcacaggcgc tcagaaatag
      421 tgcgaatgga tgcgagaaag tggtgcttac gcaccatttt ggtgcactat cagcattact
      481 ggcgcacttt gatcctgact cttatttcac gtcctccccg gatcaatctg tctgcgtcgg
      541 ccttcccttc ggcggccgct tcttcttcac cttccgtcat cgagatgagc tcgtgcccgt
      601 ggacccggca ccaggccgga atgtcctgca tcacgcctgg gtcggtggca acgatctcga
      661 caacggtgcc cggtgggagc cctttgacct tttcctgggt tcttatcacc ggcagggggc
      721 acagtaaccc ccgagcatcc aatgtttcaa gctccgacat gccaatcggt cccaatatcg
      781 gcctcggcga gcggcgcaac gctcaggcgg gtttccgcgc cgcctggctc cgcggcgatc
      841 acctcaaccc tggctgcatc tctgccttga ccgtatcggg ccaggatacg tgctgccagc
      901 tcgatgtcat cggcgccggc gtctccgtcg atgagggcca gcggcccctt gtggctgatg
      961 gcggtcaggc tcgtgaactg gtggcggtag ccctgcagaa accggttctc gccgagctcg
     1021 cggccgatga tcaacttgaa atggggccgc ggccgcaggt ggcggccgac cttcagcagc
     1081 atgatgtcgt ccagctcgta gtctcgcgat ccctttgcct gccagagatc ggtcagcttg
     1141 gccgagtact gcgcgttcgt gaggaaacag caccctccag ccggctggga ccagtcctcg
     1201 aagccgaagc ggcgcgccag agcgaactgc ggcttgcggt tgcgtccatg gaagtcgtag
     1261 agggcttcgc gatcgaccca gccctcgcgt tcaggcagcg tcgggggaag gtgcttggcg
     1321 catagcggtc tgagcagccg gtcgtctgct ccggactccc gcgcaatgat cggcatggtt
     1381 tccttgcgct ggcttttcgg acgctgaccg atgacctccc cggtgacgat gaagtcaaag
     1441 ccgttttcct ccatgaagct ccaggccagc gccttgcgca ccatgaatac tttgcagtcc
     1501 aggcaggggt tcagatgagc cccgtagccg tgcttcggat tgagcaggac gtccttgtac
     1561 tcgtcgatga cgtcgatgat gtgcagcctg ataccgagct gctcggcaac ccacagcgcg
     1621 ttgttgcgct tgggcttgtt gcggtccttt tcccggatgg cgtgagtgtg gccctccacg
     1681 cagaagcccg tgaagaagtt gacgccctcc acgtggacgc cctgctccat gacgaccttt
     1741 gccgccagca tggagtcgag accgcccgaa attaacgcga cagcccttcg ctgttccgcc
     1801 ataattcgcc acctttttgg ccgcggcata accgctgccg ctgctgcccg attgatgctc
     1861 ccccgccgca ggcgccgttc atgccgtctg tcggctttgg tgcttcaagc cgcgcattgt
     1921 aatggatcaa tgaacactga ccacctgcgc aacgtcgcca tcatcgccca tgtcgaccat
     1981 ggcaagacca cccttgtaga ccggctgctg cagcagaccg gctcgctcga ccgcgaccag
     2041 cccgagcggg tgatggacag caacgagctc gaacgggagc gcggcatcac cattctgtcg
     2101 aagaacacgg ccatcgagta cgaaggctac cggatcaata tcgtcgatac cccgggtcat
     2161 gcggatttcg gcggcgaagt cgaacgcatc ctctccatgg tggactcggt cctcctgctg
     2221 gtggacgccg tcgaagggcc catgccccag acccgcttcg tgacggagaa ggcgttcgcc
     2281 gcggggttga gccccatcgt cgtggtcaac aaggtggatc gtcccggcgc cgatccttac
     2341 cgggtaatcg atcaggtgtt cgacctgttc gacagcctcg gggcgagcga cgagcagctc
     2401 gactttcccg tggtctacac ctcggcgctg ctggggcagg cggggcccga gcccgacgcg
     2461 ctggtagacg acatgacccc gctgctgcag agcatcatcg cccacgttcc gcctcccagc
     2521 gtggacgcgg acgggccgct gcagatgcag atcagctccc tcgactactc cagctacgtc
     2581 ggcgtgatgg gcgtgggacg catacgtcgc ggacggctcg cgcgcaacac cccggtggtc
     2641 gttgtggatc gcaatggccg gacgcgtaaa gcacgggtgc tggccgttct cggctaccac
     2701 ggcttggagc ggatcgagag ggaggaggca ggcgcgggcg acatcgtgtg cgtgacgggc
     2761 gtcgaggaga tcggcatttc ggacacgatc tgcgcgcccg agcacgtcga agcgcttcct
     2821 ccgctgactg tggacgagcc gaccctgacc atgatgttct gcgtgaacac ctcccccttc
     2881 gccgggcgcg acgggtcgtt cctcacgagc cgccagctgc gcgagcgatt gctcagggaa
     2941 gcctctcaca acgttgcgct tgcggtggcc gaaaccgacg acccggatcg gttcgaggtc
     3001 tcgggccgcg gcgagctcca cctctcggtg ctgatcgaga ccatgcgcag ggaaggctac
     3061 gaggtggcgg tctcgagacc ccgggtcatc atgaaggaag aggatggtcg gttggtcgaa
     3121 ccctacgaga cgatcaccat cgacgtggac gatgagcacc agggcaaggt gatggagagc
     3181 cttggtgagc gccgcggcga gctgcaggag atgcacccgg acggccatgg gcgggtgcgg
     3241 ctgcgctacc ggattcccac gcgcgggctg atgggctatc gccctcagtt tctgtccatg
     3301 acgtcgggca ccggcatcat gacgtacgcc tccgccgggt acggtccgcg cttcgacgga
     3361 gagatcggcc gccgcacgaa cggggtgctc gtctccaacg ccaagggcaa ggcggtggcc
     3421 tacgcgctgt tcaacctgca gaaccgcggc cggctgatga tcggtccgaa cgatgaggtt
     3481 tacgaaggca tggtggtcgg cgtgcacagc cgcgacaacg acctgacggt caaccccatg
     3541 aaggagaaga agctgaccaa tattcgcgca tcgggcagcg acgagaatat tctgctcacc
     3601 acgccgatca ggctcgggct ggagcaggcg ctcgagttca tagacgatga cgagctggtc
     3661 gagatcacgc caaccgcgat acgcgtccgc aagcagtggc tgaaagaaca tgagcgcaag
     3721 cgcgctggct gaaaccgttc ccgatcgggg gcgtgtttag atgcgcgtcg tcgttcagcg
     3781 ggtttccagc gcgtcggtga ccgtcgacga cgaggccgtc gcgtctatct caacggggtt
     3841 gctggtcttc ctgggggtgt tccccgacga tggtgacgcg cagctccact ggatggccga
     3901 caaggttctc ggactgcgcg tctttcccgg tgatgacaag ccgatgaacc agtctgtggt
     3961 cgatgtcgcc ggcgaggtgc tcgtcgtgtc ccagttcacc ctcgcggcag acacgtcccg
     4021 gggcaaacgc cccggattca gcacggccgc gccaccggac ctggcggaga ccatctatga
     4081 gcgcttcgtg gctcggctgg cggcgttgta cccgcgggta gcgtccgggc ggttcggtgc
     4141 ggacatgaag gtggcgctcg tcaacgacgg cccggtgacc ttccttctcg accgctgatc
     4201 cgcggattca gactggtccc atgggtcgct cctggatccg gcgggagggc gacgatctca
     4261 acccggctcg ttgctccggc gctcggccag ccgggcaaag acgatcccga cctcgaagag
     4321 cacccaggtc ggcagggcca gcagcagctg ggagatcacg tcgggcggtg tgagaagcat
     4381 accgactatg aagcagccga cgatcacgta tggacgtttg ttagccatgc cgtcggcggt
     4441 ggcgatgccc gaccaggcca gcatgaaagt ggcaatggga atttcgaacg cgaagccgaa
     4501 cgcgaagaat atcttgagca cgaaatccag atatcgattg atgtcggtca tcatggtcac
     4561 gccctccggc gtgacagcgg ccatgaactg aaagaccagg ggaaagacca gaaaatacgc
     4621 gaacgcgacg ccgccgtaga aaagcaccat gctggacagc agcacgggga cggcgaactt
     4681 cttctcgtgc aggtacagcc ccggcgagat gaacgcccac atctggtgca gcagaaacgg
     4741 catgccgatg aacacggcgg tgaagaccga cagcttgaag ggcgtgagga atggcgacgc
     4801 cacctcggta gcgatcatct tgccgcctcc gggcaggtgc gccaacagcg gagctgcgac
     4861 cagctcgtac aggtcgtttg cgtagtagta ggtagggaag aagagcagca ggaccaccag
     4921 aaggccgcgc aggatgcgcg atctgagctc gatgacgtga tcgagaaacg gctgctcgtc
     4981 ggcgtctacc tgctcgtcgt ggctgagccg gttgtcggaa tcttcagcgc ccgccattgt
     5041 ccgcagtggc cgaggtttcg tttgaaggcg ggtttccggc agcctcggtc tgagcatcgc
     5101 cggccgggct gtcggcggtt ccacgcacct cgccctcgat gcgcttcatc tcctccatga
     5161 tggcctcgtt gtggagttgg cggcgcacgt cgtccatgcc gatctccttc tcgatctccg
     5221 tcttcacagc ggtgaagctg cggcgcattc tgccgagcca cagtcctagg gtccgcagcg
     5281 cttcgggcag ccgctccggt ccgatgacca gcagcgcgac cacggcgatg agaacgagct
     5341 ccggaaagcc gatgtcgaac attgctagac gctggagtcc tgcttggtct cagtcttctg
     5401 cgccgcgttg acctcacctt cgatcacgcg gggctcctcc gcgggcgcgg ccttggcctc
     5461 ttcctcggtc acggctttgc gaaacccctt gatggcgttt cccaggtcag agccgatggt
     5521 tttcggccgc ttggggccga atatgaggac cacgatgatg aggatgacga tgagttcggt
     5581 catgcccagt ccaaacattt gctgtctccg tcaggtcgtt ggtcaagtat acgtcttccg
     5641 aacccagccc acctcgtcgg aatccaatag gcccgtcagg ggccgccggc gttgcggctg
     5701 cttttctcct cgtgcccgga gatgccgaaa cgctctcgca acgtttcgaa aacccagcgt
     5761 gggtcctgat tgagatgcac gagcatgacc agagtgtgaa accacagatc ggccacctcg
     5821 tgaacgagcg catcgtccct gtcggacgac gtcgcatcct tggcagcgag caccacctcg
     5881 gtggcttcct cgcccacttt ctcgagaatc ctgttcaggc cgtcagcgta cagcgatgcg
     5941 acgtaggacg aaccggggtc ggcgctgcgg cgggattcga gaacttcgat caggtcttcg
     6001 acgatgtctt tgctatcggt catgtttcag gccttcaaga gcagcaacga agcgatggcc
     6061 gcgctggcga gaccagccac ggtgccgaga tctccgctgc cgctcagcga ttcagcgata
     6121 gggccccata gtaggacgct ggccagcacg agcaaagcgg cgcccgccac ccggcgtccg
     6181 cgcacgcccc gggcgagcct gcccacgcgc gcctcgagct cgtgcagttc ccggttctgt
     6241 cggtccacca tcctttcgag gtggcgtatc tgccggctgg ccgtcaccac catgccaggc
     6301 aggcgcggca gctccgcgag aatctccggc gcgcgttctg ccagctcccg cagggcggcc
     6361 gccggtccca cccgctcggc catccaggat tccatgaacg gtttggccgt ctcccagaga
     6421 tccagctccg ggtacagctc ccgacccagg ccttcgatgt acagaagcgt cttctgcagc
     6481 agcaccagct gaggctgaat ctccatgtga aaggcgctgg ccgtttcgaa gagctccacg
     6541 agaaactgac cgaaagagat ctcgttgagc ggttttgcga agatgggctc gcagacctcg
     6601 cgaatgaccc gctcgaactc ttcggggtcc gtttcgtcgg gaacccagcc ggactccaga
     6661 tgcagccgcg cgacctcggc gtaatcccgg ttgaagaacg ccagaagatt tcgggcgagg
     6721 tagtcctgat cctcctggtt gagcgagccg atgatcgcgc agtccacggc aatgtagcgc
     6781 gggtcctcgg gatcggtcac gtcgacgaag atgttgcccg ggtgcatgtc cgcgtggaag
     6841 aagttgtcca cgaacacctg cttgaaaaag gtctccaccc ctttctcggc caggcgccgc
     6901 atgttcgtcc cgcgggcttt gagctcggcc acatcggaga tcggtattcc gtggatacgc
     6961 tcgatcacca ggatgttgct gcggcaccag cgccagtaga cgcgcggcac gtacaggagc
     7021 tccgactcgg cgaagttgct gcgcagccgg gcggtgttgt ccgcctcctt ctgcatgtcg
     7081 agctcaccga gaatcgtttg ctcgtagtcc ttgaccacct gcttgaggtg cagccgccgc
     7141 gcctcctcag aggcgttctc cagaatctcc gcggcggcgc gcagcagttc cagatctgcc
     7201 cggatgacgt cggctatgtc gggtctgacg accttgacca ccacgctctt gccgtccggc
     7261 aggatggcgg catgcacctg ggccacagac gcggatgcca tgggctctat ctcgaaggag
     7321 cggaaggctt cgctgatggg ttggcccagc gcgtcctcga cgatccgcac ggcctgctcg
     7381 ccgggaaacg ggggcacccg atcctgcagg gcgctgagct cgtccgcaat gtccagcggt
     7441 atcagatccc gccgcgtgga aagcagctgt ccgaatttga cgaacaccgg cccgagctgc
     7501 tccagcgcca gcagcaggcg ccgcccgcgc ggttcgtcgc cgaccggtag aagccgggcg
     7561 ggagaagcgc gcagtagcca ggcgtagcga cgcggcaacg ccgcgccttc ctgatccgcg
     7621 tcgatcaggg tgtcgagccg gtagcggctc acgatccaga gaacccgaaa gagtcggcgc
     7681 agagcgctca tggcgttgga ggttccgggc gctggtggga gccccgggcg ctctccagat
     7741 tgcctatgcg tgcggcgagg cggtccgatc gttcgcgggc atcttccagc cgatcgagaa
     7801 gaacgttgac gcgggtgcgc gtcgcaaaac gctcgcccac gccgcgccgc agggattcgc
     7861 ctgtagcctc tgctgcagat ttcagcaggg ccagacctgc ctccgccgcg ttcagaatgc
     7921 cgtctgctga ggtttctcca accagcttcg acagcggctc ggccaggtcg ggtgcgaagc
     7981 ccttgaagat ggccgccagc tcctccagca cgctgacgtc gccttcgatg gtgaccggcc
     8041 cggcgcttct gcctccggcg agcatggaaa gcagagccgg aacggaaccg cgcacgacta
     8101 cgttgggctc ggcctcggcg tgcggcgtga acgccatgcg ccccccgacg accgacagcg
     8161 tgaaggattg ctcacggccg ccgggcagcg ctgcgatgag gcgcgcccgg tgtccttccg
     8221 ccgcggccag ccgggatgcg gctttggggt ccagctcgag cgttgcgttg gccgccctgt
     8281 tgacgagctc caccagggct gattcgaaaa cgtctgcggt gctcatggct tgaccccgcg
     8341 gtgaatggcc gcgatgcccc ccagcagatt gtgatactcg acggcggcga agccggcgtc
     8401 ttcgagcatg agcttgagcg ccttctgcgg gggatgattg cgaatcgact cgatgaggta
     8461 ctggtagctt cgggcgtcgc cagccaccag cttgccgagt ccgggccaga gcgactgaaa
     8521 cgtcccgtag gccccctcca gaaatgcgtt ctccggcctg gagaactcca ggatcagcag
     8581 cacgccgccc ggcgcgatca cgcgattgag ctcctcgagc gcaacccgtt tgtcggtgaa
     8641 gttacgcaat ccgaacccga tgctcacgca gtcgaagctg tcgtcggcga agggcagagc
     8701 ttccgccgct gcctggcaga acgagacgtt tgcgcaaccc tggtccagca gacggttgcg
     8761 gcccacgtcc atcatgcgtg cgttgatgtc cgcgagcacg acaactcccg acgggccaac
     8821 cgcaccagag aacaacgcgc tcagatcgcc ggtgccgccg gccaggtcca gcacgcgcat
     8881 gccctcccgg actcccgaga gctccaccgt catccgcttg aacaggcggt gggtgcccag
     8941 agagatcagg tcgttcatga cgtcgtagcg ttcagccacg ctcgcgaaaa ctccgcctac
     9001 caggcgggtc ttctcatctg gcgatacctt gcgggagccg aagtcgacgg agttctgatc
     9061 gctcattgat cctgctccgg ggggacggcc ggctggcttg ccggcgtcca gtggcccacc
     9121 ggtatggtgg gaagccgcga ggcgttggcc gccttcagct cgtcgagata ggacttccag
     9181 taaccgtcgg cgttctcggc gagatcccgc agaaggtccc aaccgtacag gccggtgtcg
     9241 tggccgtcat cgaaggtaat gcggatcgcg taattaccta cgggctccag gcttttgatg
     9301 ccgacgtgct tcttgcccgt ctgcagcttg cgctctgcgg ccgagtggcc ccgcacctcc
     9361 gcagagggtg agtagacgcg cagcagctcc gcggggatct ccgcgtcgcc gtcctcgaaa
     9421 gagagaacca ggacttttga ctggcggcga taggtaatct gctttgggcg ctgcatggcc
     9481 ttggcccgtc gcctacagga tgtatctgga aagatcgcgg tcctgggcca gctccgagag
     9541 ctgagcgtcc acgtagaccg cgtcgatgac caccgtgctt tccgcgctct cggtagccgt
     9601 gaacgaaacg ttttccagga ggcgctccag gaccgtgtgc aggcgccgcg cgccgatgtt
     9661 ctcggtgctc tcgttgacct gccacgccag ctcggcgatg cgctcgatgc catcgtcggt
     9721 gaattcgagg ctgagcccat cgatccccag cagcgcctga tactgctcgg tgagcgatgc
     9781 gttgggctcg accaggattc gcttgaagtc gccggcagac agggccgcaa gctctacgcg
     9841 gatgggcagc cggccctgca gctcggggat cagatcggaa ggtttcgcca ggtgaaacgc
     9901 gccgctggcg atgaagagga tgtgatcggt tttcaccatg ccgtacttgg tggacaccgt
     9961 gcagccctcg atcaggggga gcaggtcgcg ctgcacgccc tcccgggaga cgtccgcgcc
    10021 cgcgctctcg ctgcgctttg ccaccttgtc cagctcgtcc aggaacacga tgcccatctg
    10081 ctccacggtt tccacggcgc gtgccttgat ctcgtcctcg tttaccagct tcgccgcttc
    10141 ctcttccttg agcttgcgca acgcgtcctt gattttcagg cgcacggagc gttttctgcc
    10201 g
//
LOCUS       DBNHLM010000217         2897 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_570299, whole genome shotgun sequence.
ACCESSION   DBNHLM010000217 DBNHLM010000000
VERSION     DBNHLM010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_570299"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /locus_tag="AC16QF_10070"
     CDS             complement(<1..1661)
                     /locus_tag="AC16QF_10070"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR6448944.1"
                     /translation="MFNKILIANRGEIACRVIRSARSMGIPTVAVYSDADARALHVTM
                     ADEAVRIGEAPSTESYLRMERIIEACRATGADAVHPGYGFLSENAQFREALDEAGIVF
                     IGPGTRAINAMGDKITSKKLAQEAGVNTIPGYTGVIDGPDHAVEIAREIGYPVMLKAS
                     AGGGGKGMRVVRDDDECRDGFERARNEARSSFGDDRVFAEKFIEDPRHIEIQVMADSH
                     GNVIYLGERECSIQRRHQKVIEEAPSPFLDEATRQAMGEQAVALAKAVDYESAGTVEF
                     IVDAERNFYFLEMNTRLQVEHPVTELVTGQDLVELMIKVAAGEPLPLSQADVTLKGWA
                     IETRVYAEDPFRNFLPSIGRLVHYRQPIETSSVRVDTGIVEGSEVSMFYDPMIAKLIT
                     YGEDRDQAIDRMADALDAYYIRGVSHNISVLNALISHPRFREGRLTTNFIAEEYPDGF
                     HATDLPPTDAMLPIAIVAAVHRAHVERSANISGQTRRFERQIGDDWIVIANGTHYPVN
                     VTAVEHGYEVTYEDSIYRIKTDWRIGDPLVVAEVNGRLVTVQVDRT"
     gene            complement(1681..>2897)
                     /locus_tag="AC16QF_10075"
     CDS             complement(1681..>2897)
                     /locus_tag="AC16QF_10075"
                     /inference="COORDINATES: protein motif:HMM:NF013225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR6448945.1"
                     /translation="KICKVMDQAMKVGAPLIGLNDSGGARIQEGVASLGGYAEIFQRN
                     VLASGVVPQISLIMGPCAGGAVYSPAMTDFIFMVKDSSYMFVTGPEVVKTVTHEVVTP
                     EELGGAKVHSGKSGVADLALENDVEALLATRRFVDFLPPNNREKPPRLTTDDPANREE
                     PSLDSLVPADPAKPYDIKEVIVKVADEADFFEIQPDFAKNIVVGFIRLQGSTVGVVAN
                     QPMVLAGCLDIDSSKKGARFVRFCDAFNIPILTLVDVPGFMPGTAQEYGGIIKQGAKL
                     LYAYAECTVPKVTLITRKAYGGAYDVMASKHLRGDVNFAWPSAEIAVMGAKGAVEIIF
                     RKDLGDPEKIQARTDEYQQKFANPFVAASRGYVDDVFLPRETRQRICRSFAMLRDKKL
                     ENPWRKHGNIPL"
BASE COUNT          471 a          912 c          930 g          584 t
ORIGIN      
        1 gtccggtcca cctgcaccgt gaccagcctg ccgttgacct cggctaccac caggggatct
       61 ccgatgcgcc aatccgtctt gatgcgatag atcgagtctt cgtacgtcac ctcgtaaccg
      121 tgctccaccg ccgtgacgtt caccggatag tgcgtaccgt tggcaatcac gatccagtcg
      181 tcgcctatct gtcgttcgaa gcgccgtgtc tggccgctga tattggcgga acgttccacg
      241 tgagcgcggt gcacggcggc aacgatggca atcggcagca tggcgtccgt cgggggcagg
      301 tcggttgcgt gaaagccgtc ggggtactcc tcggcgatga agttggtagt gagcctcccc
      361 tcgcggaagc gcgggtggct gatgagcgcg ttcaggacgc tgatgttgtg ggagaccccc
      421 cggatgtaat aggcgtccaa cgcgtcggcc attctgtcga tggcctggtc acgatcctcg
      481 ccgtaagtga tcagcttggc gatcatggga tcgtagaaca tcgaaacttc actgccttcc
      541 acgatcccgg tatccacgcg cacgctgctg gtttcgatgg gctggcgata gtgcaccagg
      601 cggccgatcg aaggcaggaa attgcggaac gggtcctccg cgtatacccg cgtctcgatg
      661 gcccagcctt tgagcgtcac gtcagcctgg ctcagcggca gcggctcgcc ggcggccact
      721 ttgatcatga gttccaccag atcctggccg gtaacgagtt cggtgaccgg atgctccacc
      781 tgcaggcggg tgttcatctc gagaaagtag aagttgcgct cggcgtcaac gatgaactcc
      841 acggtgccgg cggactcgta atcgacggct ttcgcgagag cgactgcctg ctcgcccatg
      901 gcctgacggg ttgcctcgtc gaggaacgga gagggcgcct cctcgatgac tttctgatgg
      961 cggcgctgga tggagcactc gcgctctccc aggtagatga cgttgccgtg gctgtcagcc
     1021 atgacctgaa tttcgatgtg gcgcgggtcc tcgatgaact tctccgcgaa cacccggtcg
     1081 tcgccgaagc tcgagcgcgc ctcgttgcgc gccctctcga acccgtccct gcactcgtcg
     1141 tcgtctcgaa cgacccgcat gcccttgccc ccgccgccgg cgctggcctt cagcatgacc
     1201 gggtagccga tctcccgcgc gatctccacg gcgtggtccg ggccgtcaat cacgcccgtg
     1261 tacccgggaa tggtgttcac gccggcttcc tgggcgagct tcttggacgt gatcttgtcg
     1321 cccatggcgt tgatggcccg tgtgccggga ccgatgaaga cgatgccggc ctcatccagc
     1381 gcttcgcgga actgggcgtt ctcggacagg aagccgtagc ccggatgaac ggcgtcggct
     1441 ccggttgccc tgcacgcctc gatgatccgc tccatgcgca gatagctttc cgtggaaggt
     1501 gcctcgccga tgcgcacggc ctcgtctgcc atggtgacgt gcagcgcccg agcgtccgcg
     1561 tccgagtaca cggcgacggt gggtatcccc atgctgcgag cggaacggat cacccggcat
     1621 gcgatttccc cgcggttcgc gatgagtatc ttgttgaaca tgctgctcgc cgcctccgct
     1681 ctacagggga atgttgccgt gcttgcgcca ggggttttcc agcttcttgt cgcgcagcat
     1741 ggcgaacgac cggcagatgc gctggcgggt ctcccggggc agaaagacgt cgtcgacgta
     1801 gccgcggctg gccgccacga atgggttggc gaacttctgc tggtactcgt cggttcgggc
     1861 ctggattttc tcggggtctc ctaggtcctt gcggaaaatg atctcaacgg cgcctttcgc
     1921 acccatcact gcgatctcgg cgctcggcca ggcgaagttc acgtcgccgc gcaggtgctt
     1981 ggaggccatc acgtcgtaag cgccgccgta agccttgcgg gtgatgagcg tgacctttgg
     2041 cacggtgcac tcggcgtacg cgtacagcag cttggctccc tgcttgatga ttcccccgta
     2101 ttcctgggcc gttccgggca tgaatcccgg cacgtcgacg agagtgagaa tcggaatgtt
     2161 gaaggcgtcg cagaagcgca cgaatcgcgc gccctttttc gaggagtcga tgtccaggca
     2221 gccggcgagc accatgggct gattggctac gacgcctacg gtggatccct gaagacggat
     2281 gaatcccacg acgatgttct tggcgaaatc gggctgaatc tcgaagaagt ccgcctcgtc
     2341 ggcgaccttc acgatcactt ccttgatgtc gtacggcttt gccggatccg cgggcaccag
     2401 cgagtcgagg gacggctcct cacggtttgc gggatcgtcc gtcgtcagcc gtggtggttt
     2461 ctcgcggttg ttgggcggca ggaagtcgac gaagcggcgg gtggcgagca gcgcttcgac
     2521 gtcgttctcc agggccaggt cggcaacgcc cgatttgccg ctgtgcacct tggcgccccc
     2581 gagctcctca ggggtgacca cctcgtgggt gaccgtcttc accacctcgg ggccggtgac
     2641 gaacatgtag gagctgtcct tgaccatgaa gatgaagtcg gtcatggccg gagagtagac
     2701 ggcgccgccg gcgcaggggc ccatgatcag cgagatctgc ggcaccacgc ccgaggccag
     2761 cacgttgcgc tgaaagatct cggcgtagcc gccaagagac gcaacccctt cctggatcct
     2821 tgcgccgccg gaatcgttca ggccaatcag aggcgcgccg accttcatgg cctggtccat
     2881 caccttgcag atcttct
//
LOCUS       DBNHLM010000218         3931 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1408908, whole genome shotgun sequence.
ACCESSION   DBNHLM010000218 DBNHLM010000000
VERSION     DBNHLM010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1408908"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1281
                     /locus_tag="AC16QF_10080"
     CDS             145..1281
                     /locus_tag="AC16QF_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003116511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6448946.1"
                     /translation="MNYNLTEDQLAIKDLAERFTAEQITPNAAEWDEKHIFPRETIRA
                     AAELGFGGIYVSEASEGIGLGRIEAALIIEAMAYGCPSTSAFISIHNMAAWMIDQFGS
                     DDVRKRFVPDLVTMEKAASYCLTEPGAGSDAASLKTRAVRDGDHYVINGSKAFISGGG
                     ENEIYVCMLRTGDEGHRGISCIVVEKDTPGLSFGAQERKLGWHSQPTAMVNFEDVRVP
                     VSNRVGEEGEGFKIAMAGLDGGRLNIAACSIGGAQRCLDEAIGYVKERRQFGSAIADF
                     QNTQFMLADMATQLAAARALTHMAAQKVTENAADKTEFAAMAKLIATDSGSKIANDAL
                     QLHGGYGYLMDYPVERFVRDLRVHEILEGTNQIMRMVIGRSLVS"
     gene            complement(1340..2125)
                     /locus_tag="AC16QF_10085"
     CDS             complement(1340..2125)
                     /locus_tag="AC16QF_10085"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448947.1"
                     /translation="MAGSKTANEIFDLSEKVAIITGGSRGLGLNMAWAFAAAGARVIV
                     ASRKQEVCDAAAQEIQAGTGQECLGAAYHAGSWQDGDRLAELVYARFGRVDVLVNNAG
                     MSPLYRSLPDVSEALYDKVLDVNLRGPFRLSALIGARMAEGDGGSIINVSSTAAVQPT
                     PTELPYAMAKAGINAFTLGLARAYAPKVRVNCIMPGPFLTDISKAWDMDAFNERAKTT
                     IPLQRGGEPDEIVGAALYFASAASSYTTGSVLKVDGGSAYPAA"
     gene            complement(2216..3085)
                     /locus_tag="AC16QF_10090"
     CDS             complement(2216..3085)
                     /locus_tag="AC16QF_10090"
                     /inference="COORDINATES: protein motif:HMM:NF006140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR6448948.1"
                     /translation="MSAASVEYKAVLYEQPTERIARIVLNRPEMANAQNLQMLYDLNA
                     ALDQAASDDAVRVIIVAAAGKHFSSGHGPMGGEGDVSLKDVALSTSRGFKQPGIEGHW
                     AFEEEVFFNLCWRWRNIPKPTIAQVQGKVIAGGLMLVWPFDLVVASEEATFQDPVVAF
                     GVNGVEYFGHPWEFGVRKAKEMLFTGRAITARVAKELGMVNRVVPLEDLADETLDMAR
                     EIAEQPMMGLKTAKESINQAQNEQGLFSALRSAMTLQQLSHANNNMVHGMGVVPEGAR
                     QVRDLIKKPPLDE"
     gene            complement(3198..>3931)
                     /locus_tag="AC16QF_10095"
     CDS             complement(3198..>3931)
                     /locus_tag="AC16QF_10095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012724628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6448949.1"
                     /translation="DSYVVNGQKVWISRIQHSDLMVLLARTTPKEEVTKKTEGLSAFI
                     VDVKAAEGNGLTIRPIESMINHHSCELFFDDLVIPRESLLGEEGRGFKVILDGMNAER
                     ILIAAECIGDGRYFIDKACAYARERMVFDRPIGQNQGVQFPIARCYADVEAASLMVDK
                     AARMFDAGQPCGAEANMAKMLASESSWRAGDVCMQTHGGFAFAKEYHIERKFRETRLY
                     QIAPISTNLILSFIGEHVLDLPRSF"
BASE COUNT          745 a         1292 c         1169 g          725 t
ORIGIN      
        1 tcatcgacct cggacgatcc tccctgctag ggatatcgcg cgcaaccccg gggcatctga
       61 acggttgcgc gtcgcatcgg tatctgaggg ctgtgcaatg cattagaatc ccccgctgcc
      121 cgcgacccga cggatccaaa cccgatgaac tacaacctca ccgaagatca actcgccatc
      181 aaggatctgg ccgaacgctt cacggcggag cagattacgc ccaacgcggc cgagtgggac
      241 gaaaagcaca tcttcccgcg ggaaaccatc cgcgcggccg ccgagctagg gttcggagga
      301 atctacgtat cggaagcctc cgaaggcatt ggccttggac gcatcgaggc cgccctcatc
      361 atagaagcaa tggcctacgg ctgtccctcc acatcggctt tcatctcgat ccacaacatg
      421 gccgcctgga tgatcgacca gttcggctcc gacgacgtca gaaagcgctt cgttcccgac
      481 ctggtaacca tggagaaggc ggcgagctac tgcctcaccg aaccaggagc cgggtcggac
      541 gccgcatcgc tgaaaacgag agccgtgcgc gatggcgatc actacgtcat caacggcagc
      601 aaggccttca tttcaggcgg tggcgagaac gaaatttacg tgtgcatgct gcgcacggga
      661 gacgagggac atcgcgggat ttcctgcatc gtcgtcgaga aggacacgcc cggcctgagc
      721 ttcggcgccc aggagcggaa gctcggctgg cattctcagc cgacggcgat ggtgaacttc
      781 gaggacgtgc gagtgcctgt ttccaaccgg gtgggcgaag agggcgaagg ctttaagatc
      841 gccatggccg ggctggacgg cggccggctc aacatcgccg cctgctcgat cggtggcgcg
      901 cagcgctgcc tcgacgaggc cattggctac gtgaaagaac gccggcagtt cggctccgcc
      961 atcgccgact ttcagaacac tcagttcatg ctcgccgaca tggccaccca gctggcggca
     1021 gcacgtgcgc taacccacat ggccgctcag aaggtgacgg agaacgcggc cgacaagacc
     1081 gagtttgccg cgatggcgaa gctgatcgcc acggattcgg gctccaagat cgccaacgac
     1141 gccctgcagc tccacggcgg gtacggctac ctcatggact atccggtcga gcgcttcgtc
     1201 cgagatctcc gcgtccatga gattctggaa ggcaccaacc agatcatgcg gatggtgatc
     1261 ggacgatcgc tggtgagctg acggccgcca ggccccgctg cggcaatcgc caggtcccgc
     1321 aaccaaggcc cgaccgcact caggctgcag ggtatgcgct gccaccgtcc accttgagta
     1381 cggacccggt cgtgtagctg gaggcagccg aggcgaagta cagcgcagcc ccgacgatct
     1441 catcgggctc cccgccgcgc tgcagcggaa tcgtcgtctt tgcgcgctcg ttgaacgcat
     1501 ccatgtccca cgctttgctg atgtccgtca gaaagggccc cggcatgatg cagttgaccc
     1561 gcaccttcgg cgcgtacgcc cgggcgaggc caagcgtgaa cgcgttgatc cccgccttgg
     1621 ccatggcgta gggcaattcg gtcggcgtcg gctggacagc agcggtgctg ctgacgttga
     1681 tgatcgatcc gccgtcgccc tcggccatgc gcgcgccgat cagcgccgag agccggaaag
     1741 ggcccctgag attcacgtcg agaaccttat cgtaaagcgc ttcgctcacg tccggcaggg
     1801 agcgatacag aggcgacatg ccggcattgt tgacgaggac gtccacccgc ccgaaacggg
     1861 catataccag ttctgccagc cggtcaccgt cctgccagga gcccgcgtgg taggccgctc
     1921 ccaggcactc ctgacccgtg ccggcctgga tctcctgcgc tgccgcgtcg cagacctcct
     1981 gctttcggct ggcaacgatc acccgggcac ccgccgcggc gaaggcccag gccatgttca
     2041 gacccagtcc ccggcttccg ccagtgatga tcgccacctt ttccgaaagg tcgaaaatct
     2101 cgttcgccgt tttcgatccg gccatggtca tcttgctcct ctaacccgtt tccactgtgc
     2161 cgccgtcact cgtcagcgcc aagctgacgg gttgccggct cctgcgccgc cgcggtcatt
     2221 catctaaagg cggtttcttg atgagatccc tcacctgccg ggcaccctcg ggcaccacgc
     2281 ccataccgtg caccatgttg ttgttggcat gggacagctg ctggagagtc atggcgctgc
     2341 gcagagcgct gaacaggcct tgttcgttct gagcctgatt gatcgactcc ttggccgtct
     2401 tgagccccat cattggctgc tcggctatct ccctggccat gtcgagagtc tcgtcggcga
     2461 gatcctcgag cggcaccacg cggttgacca tccccagttc cttcgccacg cgcgctgtta
     2521 tggcgcgccc ggtaaacagc atttccttgg ccttgcgcac tccgaattcc cagggatgcc
     2581 cgaaatactc caccccgttg accccgaagg ccacgacggg atcctgaaag gtggcttcct
     2641 cgctggccac caccagatcg aaaggccaga ccagcatgag cccgccggcg atcaccttcc
     2701 cctgcacctg ggcaatggtg ggtttgggta tattccgcca gcgccagcag aggttgaaaa
     2761 agacttcctc ttcgaacgcc cagtgcccct cgatgccggg ctgtttgaat cctctggacg
     2821 tgctcaatgc aacgtctttg agggaaacgt caccctcccc gcccatggga ccgtgcccgg
     2881 acgagaaatg cttgccggcg gccgccacga tgatcaccct cacggcatcg tccgaggctg
     2941 cctgatcgag cgccgcgttg agatcgtaga gcatctgcag gttctgggcg ttggccatct
     3001 ccgggcgatt cagaacgatg cgtgcgatac gctcagtcgg ctgctcgtaa aggaccgcct
     3061 tgtactcaac actggctgcg ctcatggatt tctccctaac ggactaaaaa tcttcgacct
     3121 cccgggcaca cggcctcttg aggaccaccg cggcgtggcg acggcacgcc cggctctccg
     3181 gcactggaca tgaacactca gaaggagcgc ggcagatcga gcacatgctc gccgataaag
     3241 ctcaaaatga ggttcgtgga tatcggcgcg atctggtaga gccgggtctc gcggaacttg
     3301 cgctcgatgt gatactcctt ggcgaaagcg aagccgccgt gggtctgcat gcacacatcg
     3361 ccggctcgcc acgagctttc agacgcgagc atcttcgcca tgtttgcctc agcgccgcac
     3421 ggctgaccgg catcgaacat ccgggcggcc ttgtccacca tcagggaagc cgcctccacg
     3481 tccgcatagc agcgcgcgat gggaaactgg acgccctggt tctgcccgat gggtcgatcg
     3541 aacaccatcc gctccctggc gtacgcgcag gctttgtcga tgaaatagcg cccatcgccg
     3601 atgcactcgg cggctatgag gatgcgttca gcgttcatgc cgtcgagaat caccttgaat
     3661 ccgcggcctt cctcaccgag caggctctca cgaggtatga cgagatcatc gaagaagagc
     3721 tcgcaggagt gatggttgat catggattca atcgggcgga tggtcaggcc gttgccctcg
     3781 gcagccttga cgtcgacgat gaatgcggag agcccctcgg tcttcttggt cacctcttcc
     3841 ttgggcgtag tgcgcgccag aagcaccatc agatctgagt gctgaatccg gctgatccag
     3901 actttctgcc cgttgaccac gtaggaatcg c
//
LOCUS       DBNHLM010000219         7169 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_270790, whole genome shotgun sequence.
ACCESSION   DBNHLM010000219 DBNHLM010000000
VERSION     DBNHLM010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7169)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_270790"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..3507)
                     /locus_tag="AC16QF_10100"
     CDS             complement(178..3507)
                     /locus_tag="AC16QF_10100"
                     /EC_number="3.1.11.5"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR6448950.1"
                     /translation="MTDRRARAEAVEPDRSVIVQAPAGSGKTTLLVERFLGLLGTVEA
                     PEEVLAITFTRKAAAEMRERVLRFLDPDFQSDEPHEQGPLNRAREVRDRVAAWRLLEN
                     PQRLLIRTIDSFNYYLARTMPVASALGPVPAPADDTASLYREAARGVLSLVGTSDGVA
                     PALELLLDWRDHQSQGVEDLLAGLLAQREQWLRAAGIAGDAERGVLEAALEEVVRGRL
                     ERADAALNAALSAAGFSSAELAGLLRVAARTLRDENRESTTLVFLEADGLPRPDPERI
                     DHWNALVAALLTANLSKPAFRRSVNVSNGFQRQSEEKAEFEALLGAFGENAGLLEALH
                     RARGLPAPRYDDESWQVLDALVQVLKRAAVELETLFARRGRTDYAGLAAAALRGLGDE
                     DGVTDLGLYLDRRIRHVLVDEFQDTNFSQLHLLEKLTAGWEPGDGRSLFLVGDPMQSI
                     YRFREAEVGLFIRVRDQGLGPLSLESKRLASNFRSREEIVRWVNDHLGPIFPEVEDAA
                     AGAVAYAPSDGERGAGGAVEVLARSERSAEAEAVADLVSRALDDHRDDAEYRAAIIVR
                     ARSHLAEILPALVRRGVPYRAVKLDPLGNRPVVQDLLALTRVILQPADVAALLAVLRS
                     PVCGLTLADLHALAGDGRSPLEADALARLAGEPRRRAARVFDVLGDARLAWRRFSVRA
                     LVDGAWRRLGGPECCPDEAARRDAAVYLDALDAAERGGLLEDWNDFRESLAGRHAEGD
                     PQSEGVKLEVLTMHGAKGLEWDLVVLPALDRAPGRPASTMLHWLPFLEADGREEVLLA
                     PLRAAEVAQDPPLVKLIRAEQQVRERYENQRVLYVACTRARERLVLSATIDPDKDPFT
                     PPSGSLLADLWATAGAEFEAALDTSGGAAAGPTVPDQRLNRIAADWEPPLGDRVAWKP
                     ELETRERAAPVEYNWAGVQARRIGTVVHRLLERVGQVGAEDFTDAHREHLLTRIPVLL
                     QSMGTRPDDLDRSAAVVADALARTLDSETGRWLLSGSHEEGACELPIAGMIDGRLVNA
                     VVDRTFVDADGVRWIVDYKSGYHAGSDLEDFFAQERERYQEQLDGYRRLFEQLEDRPV
                     KTALYLPRHGRLEVVAPAGYP"
     gene            complement(3500..6136)
                     /locus_tag="AC16QF_10105"
     CDS             complement(3500..6136)
                     /locus_tag="AC16QF_10105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR6448951.1"
                     /translation="MSPIAPLVVTPTARLARTLSRRVALERAAGGAGAWVPPRIQSFP
                     AWLANQREDYFLTATDHRVPIGAHQALWLWQSLIDRQVFIGDPEVAALAQRAWRLIHE
                     HALEAPERWPELMLSEDARAFRGWAAAYRTECDRRGVVDEWAFAGELPGLIEAGHLAI
                     PQGLELAGFDLPMTPLQVRIFDALEAMGCAVSGRPEGSASRTSQTAAMELIRFHLPDD
                     ELRGAAGWARSVLEAEPGRTVAVVVPDLAGRLERVESIFREVFDPPASVLGPRGAEPF
                     HVSLGRRLSEWSLVADALAFLELGSQRLTQPQAGRLLRSPFLAGWPDETLDRSRALAR
                     LVSRAPFEVTVNELRWALDGVGAAALDEALQSWQTLREAAAQSAWPSDWAGRFQQELS
                     ALGFASGRPLDSREYQVLNRWHDLLESFGALDVVAAGPWSRTQALRQLAERARSSVFR
                     ERNPGVPVEVLGVEEALGSSFDAVWITTLDSATWPGSPVREPLIPAAVQASVPRASGD
                     GCLARAQLELDALCASAPDVRGSYARGVEDQALEITGLLRHLPVIDAPPPTPAPGARF
                     AEPFEDHEAPKRPAGPVSGGTGVLRDQSDCPFRAFARWRLGAVDLTPPRPGLDAAQRG
                     TVIHRALERFWEDLDGQAALQGLDRDAREHRIAAAAAAAVDDLVSRHRAILGPGARRL
                     EERRVARALTRWLGLEVQRAGFEVVDREGEVAMTFAGLELEGKIDRLDRLPDGRLMLI
                     DYKTGRTSRNAWLPEARIVDPQLPAYAVSMPSAPAAITFARIRADDSRFDGIADGDPG
                     TAGVIPLADAGPRYGELGSWQALLSGWRGLLDALAQDFRSGRAAVDPRDADACKTCHL
                     HALCRIRDRDPYRDGEGGSEDD"
     gene            complement(6210..6851)
                     /locus_tag="AC16QF_10110"
     CDS             complement(6210..6851)
                     /locus_tag="AC16QF_10110"
                     /inference="COORDINATES: protein motif:HMM:NF018717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR6448952.1"
                     /translation="MSRPAYMIIAIDVRDPSRMAEYAGGTQPLLEAAGARVLAATNGI
                     TVEDGVWARERIALLEFPSMESARSFWSADAYAPLKALRESISTSDVVLVEGTQDERV
                     GEEDGAHYLLGASTMLNADWVEEYIQKVPPISARFGVKMLAGGPEFETLEGAWPGESV
                     VVLRFPSEHAFREFWYGDDYRPMKELREANAPGHHISFAETFELPDAGGPSQG"
     gene            6959..>7169
                     /locus_tag="AC16QF_10115"
     CDS             6959..>7169
                     /locus_tag="AC16QF_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448953.1"
                     /translation="MAEAIPTREIQSWPEQALRGHRIPGSRYTSKEFAEKEWEGMWTK
                     VWLLLGREEELPNPGDWQCEEVGPES"
BASE COUNT         1243 a         2568 c         2354 g         1004 t
ORIGIN      
        1 tcatcgacgg gaacgccgat cagattgccg ccttcatcga ccgggcccgg gatgacggcc
       61 acgagttcgt cacggacatc gcgcccgagt gcctgcccat cgtgggaggg gccatcaccg
      121 ccgacctggc ggccttgacc ggctcacgtt agaaccatct cctgcgggcc gcggcgttta
      181 cgggtaaccg gctggagcca ccacctccaa ccgcccgtga cgcggcaggt acagcgccgt
      241 tttcacgggg cggtcctcga gctgctcgaa aagccgccgg tagccgtcga gctgttcctg
      301 atagcgctcg cgctcctggg cgaaaaagtc ctccagatcc gacccggcgt ggtagccgga
      361 tttgtagtcc acgatccagc gcacgccatc cgcgtccacg aaggtgcggt cgacgacggc
      421 gttgaccagt cgcccgtcga tcatgcccgc gatgggaagc tcacacgcgc cttcctcgtg
      481 gctgccgctc aagagccagc gcccggtctc gctgtcaagg gttcgtgcca gcgcgtcggc
      541 aaccaccgcc gcagaccgat ccagatcgtc gggtcgcgtt cccatggact gcaggaggac
      601 gggtatgcgg gtcagcagat gctcgcggtg ggcatcagtg aagtcctcgg cacccacctg
      661 acccacccgc tccagcaacc ggtgcaccac cgtcccgatc cggcgcgcct gcacaccggc
      721 ccagttgtat tccaccggag cggctcgttc ccgggtctcc agctccggct tccaggccac
      781 ccgatcgcca agcggcggct cccagtctgc ggcgatccga ttcagcctct gatccggaac
      841 cgtgggcccg gcggcagcgc caccgctggt gtcgagggcg gcctcgaact ccgcgccggc
      901 tgtggcccag aggtcggcga gcagactgcc tgagggcggc gtgaaagggt ccttgtccgg
      961 atcgatggtg gcggagagca ccagccgctc ccgggcccgc gtgcacgcaa cgtagagcac
     1021 acgctggttc tcgtagcgct cccgcacctg ctgctccgca cggatcagct tcaccaaagg
     1081 cgggtcttga gcaacctccg ctgcccgcag aggggccagc agaacttcct cccgaccatc
     1141 ggcctcgagg aacggcagcc agtgaagcat ggtcgatgct ggacggccgg gggcccgatc
     1201 cagtgccggc aggaccacca gatcccactc caggcctttg gccccgtgca tggtgagaac
     1261 ctccagcttg accccctcgc tttgcgggtc gccctcggcg tgccgcccgg cgaggctttc
     1321 tctgaagtcg ttccagtcct ccagaagacc gccccgctcg gcggcatcca acgcatccag
     1381 atacacggcg gcatcacgcc gcgccgcctc gtcggggcaa cattcgggcc caccgagccg
     1441 gcgccaggcc ccgtcgacaa gcgcacgaac ggagaagcgc cgccaggcca ggcgcgcgtc
     1501 gcccagcacg tcgaagaccc gcgcagcccg tcgtcggggc tctcccgcca ggcgtgcaag
     1561 ggcatcggcc tccagcggcg agcggccgtc accggcgagc gcgtgcagat cggcaagggt
     1621 cagcccgcat acgggcgagc gcagcaccgc aagcagagcg gcgacgtccg caggctggag
     1681 gatcaccctg gtcagggcca gcagatcctg cacgacgggc cggttgccga gggggtccag
     1741 cttcaccgcg cggtagggca ccccgcggcg aaccagcgcc ggcaggattt ctgccagatg
     1801 gctgcgggcc cgaacgatga tggccgcccg gtattccgca tcgtctcgat ggtcgtccag
     1861 ggcgcgactg accagatcgg ccacggcctc ggcttcggcc gaccgttcgg atcgggcaag
     1921 aacctccacg gcaccgccag cgccccgctc cccgtcggaa ggcgcatagg ccacggctcc
     1981 agcagcggca tcctccacct ccggaaagat cggacccagg tggtcgttga cccagcggac
     2041 gatctcctcc cgggagcgga agttgctcgc caaccgcttc gactccagcg aaagtggccc
     2101 aaggccctgg tcccggacac ggatgaacag acccacctcc gcctcccgaa agcgatagat
     2161 cgactgcatc gggtcgccca ccaggaagag gctgcggccg tcgcccggct cccagcctgc
     2221 cgtgagcttc tccagcaggt gcaactggct gaagttggtg tcctgaaact cgtcaacgag
     2281 aacgtgcctg atgcgccggt ccagatagag gccgaggtcg gtcacgccgt cctcgtcgcc
     2341 aagcccccgc agagcggcag cagcgagccc cgcgtaatca gttcgcccgc gccgggcaaa
     2401 cagcgtctcc agctctacgg cggcacgttt gagaacctga acgagcgcgt cgagcacctg
     2461 ccagctctcg tcgtcgtagc gcggcgccgg cagtccgcgg gcccggtgga gggcctcgag
     2521 caacccggcg ttctccccga acgcgccgag gagcgcttcg aattccgcct tctcctcgct
     2581 ctgacgctga aacccgttgc tcacgttcac gctgcgccga aaggccggct tgctcaagtt
     2641 cgcggtgagc aaggcggcca ccagcgcgtt ccagtgatcg atgcgctcag gatcgggccg
     2701 gggcagcccg tcggcttcca ggaagacaag ggtggtggat tcacggttct cgtcgcgcag
     2761 cgtgcgggcc gccacccgca gcaggccggc aagctcagca gaggagaaac cggccgcgga
     2821 gagcgccgcg ttcagggccg catcggcccg ttcgagcctg ccccgcacaa cctcctccag
     2881 ggcggcttcc agcacgccgc gctccgcatc gccggcaatg cccgccgccc gcaaccactg
     2941 ctcccgctgg gctagcaggc cggcaagaag gtcctccacg ccctgactct ggtgatcgcg
     3001 ccagtccagc agaagctcca gggcgggagc gaccccgtcg gaggttccga ccaggctcag
     3061 cacgccgcgt gcggcctcgc ggtaaagcga cgccgtgtcg tcggcgggcg ccgggacagg
     3121 acccagggcg ctggccaccg gcatggttcg ggccaggtag tagttgaagc tgtcgatggt
     3181 gcggatgagc agacgttgcg gattctccag aagccgccag gccgccaccc ggtcacgaac
     3241 ctcgcgggcg cggttcaacg gaccctgctc gtgaggctca tcggattgga agtcggggtc
     3301 caggaagcga agcacacgtt cacgcatctc cgctgccgcc ttgcgggtaa aggtaatggc
     3361 cagcacctct tccggcgcct ctaccgttcc cagcaggcct agaaaacgct cgaccagcag
     3421 cgtggtcttg cccgatccgg ccggagcctg gacgatgacg gagcgatccg gctccacggc
     3481 ctcggcgcgt gctcttcgat cagtcatctt ccgagccccc ttccccgtcg cggtaaggat
     3541 cccggtcccg gatgcggcac agcgcgtgga ggtggcaggt cttgcaggcg tcggcgtctc
     3601 tcggatccac ggcagcgcga cccgacctga agtcctgggc cagcgcatcg agcaggccgc
     3661 gccagccgga caggagcgcc tgccaggagc ccagctcccc gtagcgggga ccggcatccg
     3721 ccagcgggat caccccggcg gtacccggat caccgtcggc aatcccatca aagcgcgaat
     3781 cgtccgcgcg gatacgcgca aaagtgatgg cggccggcgc cgagggcatg gacaccgcat
     3841 aggccggcag ctgggggtcc acgatgcgcg cttccggcaa ccaggcattg cgggacgtgc
     3901 ggcccgtttt gtagtcgatg agcatcagcc gcccgtcggg cagccgatca aggcgatcga
     3961 tcttgccctc cagctccagg cccgcgaacg tcatcgccac ctcgccttcc cgatccacca
     4021 cctcgaaacc ggctcgctga acctccagcc ccagccaccg ggtaagtgcc cgagcgacgc
     4081 ggcgctcctc gagacggcga gcgcccgggc cgagaatggc gcggtgccgg ctcacgagat
     4141 cgtccacggc ggccgcggcc gcggccgcga tgcgatgctc gcgggcgtcg cgatccagcc
     4201 cctgcagtgc ggcctggcca tccaggtctt cccagaagcg ctcgagggca cgatgaatca
     4261 cggtgcctcg ctgggccgcg tcgagccccg ggcgcggcgg ggtgagatcc accgcaccga
     4321 gtcgccagcg cgcgaatgcc cggaacgggc agtcggactg gtcacgcagc acgccggttc
     4381 cgccgctgac gggaccggcc gggcgcttcg gagcctcatg gtcctcgaac ggctctgcaa
     4441 aacgtgcccc gggcgccggc gtcggcgggg gcgcgtcaat gaccggcaaa tggcggagca
     4501 ggccggtgat ctccagcgcc tgatcctcta cgccccgggc atagctgccg cggacatcgg
     4561 gggccgacgc gcaaagcgcg tccagctcca gctgggccct ggccaggcat ccgtctccgc
     4621 tcgcccgcgg cacggatgcc tgaacggcag ccggtatcag cggttcgcga accggcgaac
     4681 ccggccacgt cgcgctgtcc aaggtggtga tccaaaccgc atcgaagctg gagccgagcg
     4741 cctcctcgac gccgagaacc tccacgggca cgccggggtt gcgctcacga aagacgctgg
     4801 atcgggcacg ctctgcaagc tgtcgcaggg cttgcgtacg ggaccagggc ccggcggcca
     4861 ccacgtccag agcgccgaaa ctctccagca gatcgtgcca gcgattgagc acctgatact
     4921 cgcggctgtc caggggccgc cccgaggcga acccgagagc cgacagctcc tgctgaaatc
     4981 ggcccgccca atccgacggc catgccgact gagcagccgc ctcgcgcagc gtttgccagc
     5041 tctggagcgc ctcatcgagg gctgccgcac cgaccccgtc gagcgcccag cgcagctcgt
     5101 tgacggtcac ctcgaagggt gcccgggaaa ccaggcgcgc cagcgcccgg gaccgatcga
     5161 gcgtctcgtc gggccagccg gcgagaaagg gagagcgcag caatcggccc gcctgaggct
     5221 gggtgagccg ctggctgccc agctccagaa aggccagggc gtcagcaacc agggaccatt
     5281 ccgagaggcg tctgcccagg gaaacgtgaa acggctcggc acctcgcgga ccaaggactg
     5341 atgccggcgg gtcgaacacc tcccggaaaa tggactccac ccgctcgagc cgtcccgcca
     5401 gatccgggac caccacggcg accgttcgcc ccggctccgc ttccagcaca gatcgcgccc
     5461 agcccgccgc gccgcgcagc tcgtcgtcag gaagatgaaa gcggatgagc tccattgccg
     5521 ccgtctggga agttcgggac gcagaccctt cggggcgtcc ggagaccgcg cagcccatag
     5581 cctcgagcgc atcgaagatg cggacctgca gcggcgtcat cggcagatcg aagccggcca
     5641 gctccagtcc ctgcgggatc gccaagtggc ccgcctcgat gagtcccggc agctccccgg
     5701 cgaacgccca ttcgtccacc acaccgcgcc ggtcacactc ggtacggtac gcggcagccc
     5761 agccgcggaa cgcccgtgcg tcctccgaca gcatgagctc gggccagcgc tccggcgctt
     5821 ccagggcgtg ctcgtggatg agccgccatg cccgctgcgc cagcgccgcc acctcggggt
     5881 cgccgatgaa cacttggcga tcgatgagcg actgccacag ccacagcgcc tgatgggcgc
     5941 cgatgggaac gcgatgatcg gtggcggtga ggaagtaatc ctcgcgctga ttggccagcc
     6001 aggccgggaa gctctgaatg cgcggcggca cccaggcacc ggccccgccg gccgcccgct
     6061 ccagagccac gcgacgggaa agcgttcggg cgagcctggc ggtgggcgtc acaacgaggg
     6121 gcgcaatcgg gctcacccgt gcctcacccg atcaaggcga gccgaacggc tcgatcggac
     6181 agtacccggc caagggcggg gagaccgcct caaccttggg atggcccgcc ggcgtcgggc
     6241 agctcgaagg tttccgcaaa agagatgtga tgccccgggg cgttggcttc gcgaagctcc
     6301 ttcatcgggc ggtagtcatc accgtaccag aactcccgga aggcgtgctc ggaagggaac
     6361 cgcaacacaa ccacggactc gccgggccag gcaccttcga gggtctcgaa ttccgggcct
     6421 ccggccagca tcttgacgcc aaagcgggcc gagatgggag gcaccttctg aatgtactct
     6481 tccacccagt ctgcgttcag catggtgctc gccccgagca ggtagtgggc accgtcctcc
     6541 tctccaaccc gctcgtcctg cgtaccctcg accaggacca cgtccgacgt gctgatggat
     6601 tctcgcagcg ccttcagcgg cgcgtacgca tcagcgctcc agaaggacct ggcgctctcc
     6661 atcgagggga actcgaggag ggcgattcgc tcccgagccc agacaccgtc ttcgacagtg
     6721 atcccgttgg tcgcggcgag caccctggcg ccggcggctt ccagaagcgg ctgtgtgccg
     6781 cccgcgtact cggccattcg tgacggatcc ctgacatcaa tggctataat catgtatgcc
     6841 ggtcggctca tgggctgact ccctgacgcg ctctttaagt tgcacatcat aagggaaagt
     6901 gaacgcgtta tactcggcag cccggagcga acaaaacgtc agagcgagag aaagcacaat
     6961 ggccgaagca attccaaccc gcgaaattca aagctggccc gagcaggcgc tgcgcgggca
     7021 ccgcatacca ggatcccgct atacctcgaa ggagtttgcc gagaaagagt gggaaggcat
     7081 gtggaccaag gtctggctgc tgctgggccg ggaagaggag ctgccgaacc ccggcgactg
     7141 gcagtgcgag gaggtgggcc ccgagtcca
//
LOCUS       DBNHLM010000220         3395 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1529004, whole genome shotgun sequence.
ACCESSION   DBNHLM010000220 DBNHLM010000000
VERSION     DBNHLM010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1529004"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..1471)
                     /locus_tag="AC16QF_10120"
     CDS             complement(224..1471)
                     /locus_tag="AC16QF_10120"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6448954.1"
                     /translation="MATPAQYLDKLSGTALQRVARLADDIVTGGDEAQQLRRLPDWLV
                     DKLIDEGFFRFTLPPELGGEDASTLETIEVLEAISAMDASVGWNVMLGSEINAMAAGG
                     MPKELAQEVYIDNPSVVMCGGGGPGSQPSYAIEQDDGSYRVWGETTFISGCHNATWCF
                     MGAPIIDDGQPRTDDNGMPIIKMWFLHRSQWDIIDTWDVAGLRGSGSHNVRAEGALVE
                     PKWLPVELMVTPALYDNPVFRIPVPLRLSYNKVAIALGVARGALDAFADLANNKVPML
                     SVSKLKERPIAKYRMAEATATYRAARAYVFDTMGAVEEELRKGADFPSTETTVNARLA
                     CIHAAQICKDLVDDVHTAAGTSAAYMKNPLERKLRDAHGAASHRWVAHPLYETIGGIL
                     FGEEPDAEFAGTGGPVLGGAGKK"
     gene            1566..2354
                     /locus_tag="AC16QF_10125"
     CDS             1566..2354
                     /locus_tag="AC16QF_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6448955.1"
                     /translation="MDLGIRGRKAIVNGGSAGMGRGSVLALAREGAELYVSARGAQRL
                     EEACEEIAGQTGASITPIVADHSTDEGREKILAACPEPDILVGTCAPPPVTGDYQAIT
                     ADDWRENLAVTLLSPVQFMQAVIPGMVERGWGRVVNIGTGAAKFPTQIRILSGAPRAA
                     LVNYTVAVAKAVAKHNVMINNILPGMHHTATIHERYTAAAAANGTTYDEEVQKFVDEW
                     GIPANKFGDPDDVGAFVALFCSEFANYTTGQSLVIDGGITNATF"
     gene            2415..>3395
                     /locus_tag="AC16QF_10130"
     CDS             2415..>3395
                     /locus_tag="AC16QF_10130"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6448956.1"
                     /translation="MNQLVRSPDLPDWPKQQLRGHRIDGYRYTSREFFEKEFEHMWAK
                     VWLLLGRESEIPNPGDWQREDVGPESILMVRQQDGGIKAFYNVCQHRGNRLVSEEKGH
                     VSRFVCKYHSWAFMPDGELIHAQDADDFPEGNPCGKVNLLEIPCESFAGFIWVNMDPE
                     CESLREYLGPIWDDWERYEIEKWKRYVALTTVAPCNWKVVLDNFNESYHVPTVHKPMG
                     TAAERKRMHSGVDTNYKHTRFDLSDQGHNRMIMPGGYAGATMNSEGVIGEPLASIMRE
                     WQLDPNEFVNRGQETRTALQEAKRRLGPERGYTHYARMADEMLTDAFHYTL"
BASE COUNT          610 a         1074 c         1128 g          583 t
ORIGIN      
        1 ggcaggcgcc cgtcagccga gccgggatac ccgaactcca tctcgtcgaa gagcaccacg
       61 tgctcgccca tccacaatga atccagacca gcggcctcgg cgcgccggcc catgtcgaga
      121 atggtcgccg ggctggctgc cggcgtggtg atggaggcga acgtcccgat cttcatgttt
      181 gttacctcgt ccttgcgggg ctgcgagcct tgaccggacc cgctcacttc ttgccagcgc
      241 cccccagcac ggggccgccc gtgccggcga actcggcgtc gggctcttca ccgaacagga
      301 tgccaccgat ggtctcgtag agcgggtgcg cgacccagcg atgggacgcg gccccgtggg
      361 cgtctcgcag cttgcgttcc agcgggttct tcatgtacgc cgcgctggtt cccgccgcgg
      421 tgtggacgtc gtccaccagg tccttgcaga tctgggctgc atggatgcag gccaggcgcg
      481 cgttgaccgt ggtttccgtg gagggaaaat cggcgccctt tctcagctct tcctccaccg
      541 cgcccatggt gtcgaagacg taggccctgg ccgcgcgata ggtggcggtg gcctcggcca
      601 tgcggtactt ggcgatgggt cgttccttga gcttggagac cgacagcatg ggtaccttgt
      661 tgttggccag gtccgcgaag gcgtcgagcg cgcctctggc gacgcccagg gcgatggcca
      721 ccttgttgta ggacagccgc agcggcacgg gaatcctgaa caccgggttg tcgtagaggg
      781 cgggtgtcac catgagctcc acgggcagcc atttcggctc caccagggcg ccttcggctc
      841 gcacgttgtg gctgcccgaa ccgcgcagac cggccacgtc ccacgtgtcg atgatgtccc
      901 actgggagcg gtgcagaaac cacatcttga tgatgggcat gccgttgtcg tccgtgcggg
      961 gctggccgtc gtcgatgatg ggcgcgccca tgaagcacca ggtggcgttg tgacagccgc
     1021 tgatgaacgt ggtttcaccc cacacgcgat agctgccgtc gtcctgctcg atggcgtagg
     1081 aaggctgaga tcccgggcca ccgccgccgc acatgacgac gctggggttg tcgatgtaga
     1141 cctcttgggc gagttccttc ggcatgccgc cggcagccat ggcattgatc tcggatccga
     1201 gcatgacgtt ccagccgacg gaagcgtcca tggcagaaat cgcctctaaa acctcgatgg
     1261 tctccagcgt gctcgcgtcc tcgccgccca gctccggggg cagcgtgaag cggaagaagc
     1321 cctcgtcgat gagcttgtcc accagccagt cgggcaggcg cctgagctgc tgagcctcgt
     1381 cgccgccggt gacgatgtcg tcggccagcc gagcgacccg ttgcagcgcg gtgccgctga
     1441 gtttgtccag atattgagcg ggtgtcgcca tggcgtttcc ctcagtgccg tgctccagca
     1501 gtcagtataa cgcgtttact tttcgcgcta cgatgcggaa tgtacgtatg ggaggagggc
     1561 atacagtgga tctcggcatt cgcggccgca aggccatcgt caacggcggc agcgccggca
     1621 tggggcgcgg cagcgttctg gcgctggcgc gggaaggggc ggagctctac gtctcggcgc
     1681 ggggtgcgca gcggctcgag gaagcctgcg aggagatcgc ggggcagacg ggcgccagca
     1741 tcacgcccat cgtggccgat cacagcaccg acgagggtcg cgagaagatc ctggcggcgt
     1801 gtcctgaacc cgacattctc gtgggcacct gcgccccgcc gccggtgaca ggcgactacc
     1861 aggccatcac cgccgacgac tggcgggaga atctggcggt caccctgctc agcccggtgc
     1921 agttcatgca ggccgtgatt cccgggatgg tggaacgggg ttggggcagg gtggtcaaca
     1981 tcggcacggg agcggcgaag tttcccaccc agatccgtat tctgtcgggc gccccccgcg
     2041 ctgccctggt caactacacc gtggcggtgg ccaaggccgt ggccaagcac aacgtcatga
     2101 tcaacaacat cctgccgggg atgcaccaca ccgcgacgat ccacgagcgt tacacggccg
     2161 ccgcggccgc gaacggcacc acctacgatg aggaagtgca gaagttcgtc gacgagtggg
     2221 ggatcccggc gaacaagttc ggcgatccgg atgacgtggg cgctttcgtt gcgctcttct
     2281 gcagcgagtt cgccaactac accacgggcc agagcctggt gatcgacggg ggcatcacca
     2341 acgcaacgtt ttgagcctgc tggaccaaaa gggaaacgcg ttatactatt ccgcggattg
     2401 aggagggtgg ttgattgaat cagctggtac gcagccccga tctgcctgat tggccgaagc
     2461 agcagcttcg cggccacaga atagacggct accgttacac gtcccgggaa ttcttcgaaa
     2521 aagaattcga gcacatgtgg gccaaggtct ggctgctgct cgggcgagaa tcggagattc
     2581 cgaatccggg cgactggcag cgcgaggacg ttggtcccga gtccatcctc atggtccgcc
     2641 agcaagacgg cggcatcaag gccttctaca acgtctgcca gcaccgcggc aatcggctcg
     2701 tctccgagga gaagggccat gtgagccgct tcgtctgcaa gtaccacagc tgggcgttca
     2761 tgcccgacgg cgagctgatc catgcccagg acgcggacga cttccccgaa ggcaacccct
     2821 gcggcaaagt caacctgctg gagattccct gcgagtcgtt tgccggattc atctgggtca
     2881 acatggatcc ggagtgcgaa agcctgcgtg agtatctcgg gcccatctgg gacgactggg
     2941 agcgctacga gatcgagaag tggaagcgct acgtggcgct caccacggta gcgccctgta
     3001 actggaaggt ggtgctcgac aacttcaacg agtcctacca cgtgcccacg gtgcacaagc
     3061 ccatgggcac tgcagcggag cgcaagcgca tgcactccgg ggtggacacc aactacaagc
     3121 acacgcgctt cgacctctcc gaccagggcc acaaccgcat gatcatgccc ggcggctacg
     3181 cgggcgcgac catgaacagc gagggtgtga tcggtgagcc gctggcctcg atcatgcgcg
     3241 agtggcagct ggatccgaac gaattcgtca accggggcca ggagacccgc acggcgctgc
     3301 aggaggcgaa gcgccgactg gggcctgagc ggggctacac ccactacgcc aggatggccg
     3361 acgagatgct caccgacgcc ttccactaca cgctg
//
LOCUS       DBNHLM010000221        22971 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1647834, whole genome shotgun sequence.
ACCESSION   DBNHLM010000221 DBNHLM010000000
VERSION     DBNHLM010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 22971)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 22971)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22971
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1647834"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="AC16QF_10135"
     CDS             <1..1281
                     /locus_tag="AC16QF_10135"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6448957.1"
                     /translation="QPDVPAHHIEGSRYTDRGFAAAEWDGMWSRVWLLLGRAREMPNP
                     GDYQVEDVGPESFIMVRQDDGSIRAFYNVCQHRGSRLIFNAAGSVERISCPYHSWTYG
                     RDGALLVVQDPEDFSSGNPCERLSLVEVRCETFAGWVWVNMDPDCVSLREYLGPVWED
                     WQTWQPEDWRRSLAISAWVPCNWKVIQDNFCESYHLLTVHPQLTANIEEGVPWTRFDM
                     ANEGHNRMIMQAGMPSHRQAQGPKLVEPLLSQMAMWELDPDDFAGREHDTRAAIQQQM
                     RDLGAPRGYAHYDRLRDEQLTDPHHYNIFPNCSVTFGADSVLLQRMRPHPTDPQQCRF
                     DHWTYVAPAAIEHGVFRNSDGRVEFTGDAEIKEVEYGKESMGPIADQDIGITTGQQLG
                     LRSRGYRGANLADQESRIARFHGVIDEYIAGERP"
     gene            1336..1728
                     /locus_tag="AC16QF_10140"
     CDS             1336..1728
                     /locus_tag="AC16QF_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HIR6448958.1"
                     /translation="MGDYVYPGILSSLALLVYYFTLFKAGMARGKYNIPAPSHEGPEE
                     YVRRVRVHQNTMEHLVLFLPGLWLFSYAVDPIWAAGIGVLWPVGRLWYAFAYYEAAEK
                     RGPGLYLSMPPIYIFVIGSLIGFMLELF"
     gene            1744..2772
                     /locus_tag="AC16QF_10145"
     CDS             1744..2772
                     /locus_tag="AC16QF_10145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6448959.1"
                     /translation="MPRFSILDLAPITIGGSARQALHNARDLARHGEAWGFERFWLAE
                     HHNMPGIASSATAVAIGFVAEGTSTIRVGAGGIMLPNHAPLVIAEQFGTLEALYPGRI
                     DLGLGRAPGTDQITAHALRRTLNSDVDGFPQDLMELMLYLGDPVEGQRVHAVPGAGSK
                     VPVWILGSSLFGAEVAAALGLPYAFASHFAPAAMDQAIEVYRKNFKPSEHLDAPYVML
                     GFNVCAADTEEEAVYLRSSSLQAILQLRQGAPGRLPPPVHDFDATLTPEQRYLIDRFS
                     LCSAVGTRETVERSINDFLARTGADELIIVGQIFDHQARLRSFEIVAELIGDLQPGDS
                     TRVAGQHG"
     gene            2765..3922
                     /locus_tag="AC16QF_10150"
     CDS             2765..3922
                     /locus_tag="AC16QF_10150"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6448960.1"
                     /translation="MDDELDLMGPEEMADPAVSARRLLEGPRCRLYHGFDPPFYVVSR
                     YDDVSAIVRDPGRFLSGHGQGPNFVAPMGVVSDPPQHTFFRELVQPDFLPRSIALLRP
                     RLEAIADELLDAVAEETVWDVHDRLSFPLPVTIICEIFGIPTDDIEQFKIWSDASVAA
                     LSSQNPQQYDAELARMQAYVLERLRGTRRRPGDGSLISRIARARRDGVPVTDEEAVRL
                     VLQLFVAGNETTTSLITNFVWRMLAGDGLWADFCAGRVDVDRAINESLRFDPPLLGLF
                     RTTAVDVDVGGTVIPAHSKVLTHYGAANRDPAAFRDPDVFDVGRTGRKVLSFGLGIHV
                     CLGRELALLEARVALQGLARRFPRLELVDEGERIGPFLFWGRRHLPVRNRA"
     gene            3965..4993
                     /locus_tag="AC16QF_10155"
     CDS             3965..4993
                     /locus_tag="AC16QF_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR6448961.1"
                     /translation="MCQSHDVANETENADPRSGYRLAEGWPHYPADMQFEMGSGVAVD
                     EAGVIYLFTRDIEHWAAHPLAMKDKMGKSSISMFDRAGNYLGKWGPSDERGFALGAHT
                     LYIEKDGMFWTTDRDGHTVRKYHPDGTLLLTLGEFGQWGAGTDHFNGPTAVLVQENGN
                     IVVSDGYWNSRLVWFSPEGEFIREVGTWGTGPAEFGTPHAIAQDAQGRILVADLAGGN
                     FHPYMTLPGQIAEHRTRQIPDAKSRIQILDPDGAYIGEWDHIMPLSLAVYGDHVYASD
                     KMSNLAILDAATGKVEERVDNIAIYIHQLAMDAHGDLYTASVYPEHAGEKRGPEGPSH
                     RRWTRDGA"
     gene            5167..6849
                     /locus_tag="AC16QF_10160"
     CDS             5167..6849
                     /locus_tag="AC16QF_10160"
                     /inference="COORDINATES: protein motif:HMM:NF023165.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HIR6448962.1"
                     /translation="MITGFHCSTSWVRQRARVVALLGLLTLLVAGNTARGEPAMEAVA
                     ADGSTYPVEPVALLDPAALEELVGPIALYPDDLLAIALPAATYPLQVVQASRFLQSRE
                     ENPSVQPAEGWDDSVVALLNYPEVVELLNEDLDWTWKLGEAVLAQQEDVLEAVAAFRE
                     RARLAGNLRSDDFQLVESADEGIRIRPRDPEVIYVPYYEPSRVTAYHSRPVYHYYPAR
                     YPVYYYPYAPGHRFASGFWGVTSAFSIGWTTRRLHVHYVTYRDHPYYGHHYYDRYFYR
                     RPPSFRVDDHWQWHGSFAGLGRHHAGNYWYPRAHRHGPSPRHHHRRSHHERNQDEGTD
                     REPRRHRERERERAADRPGGSEEHLADRRDPRERLVPELGPRRAGRLLARGSVVNGIP
                     VPESAATLAPRPARPPAAADSPGSAQRRPGSPIEAQQAIARARSALGARSAARGGALR
                     AQRSAGSPRAPARAMAGPTPGQQSTTASPSRSAVAVPSRPAPMAFMPRSSPPRPAVPT
                     SRRLQPAPSARTSRPSQPAPSARTSRPAFDPGTISRRGGGVRAGRPTPMRLD"
     gene            6909..8795
                     /locus_tag="AC16QF_10165"
     CDS             6909..8795
                     /locus_tag="AC16QF_10165"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR6448963.1"
                     /translation="MKKAALPENEGERLERLRDYDILDTLPEQDYDDITYLASQICDT
                     PIAVISLVDDDRQWFKSAQGLDVSETPRDVAFCAHTILEPDKLMVVEDASQDDRFADN
                     PLVAGDLGIRFYAGAPLVTASGHALGTLCVIDQKPRGLTDQQRKTLAVLARQVVALLE
                     LRRSVAELKRSSDALHESHQFLALRGQQLNRSRDELAEAVRVLEGQADVIEQDLHRAE
                     VIQRSLLPHEVPRLEDFSVRSLYRPGHTVGGDLYDVVSIADRYLAIVVADACGHGVSA
                     AMLSVLFKQHLRLQDPASGIPYPPAWALERINRSVVANRPAPGVFLTAVYALVDTQTR
                     KLKLGSAGHPPVICLRASGEVESFAHTGPALGLDESSTYEEAEIELGVGDKLLFYTDG
                     LLDICESSPTVEALGQAMVSVADKPNLLEQVLMEVTHGRARPDCDDVTMVLLDAAPGE
                     SVFNESVEGLELQEMPTTASPTISWAASEDGVFLILEGWVTWLCGETLFNAAMPLIKA
                     HRRLVIDLELCEHMDSTMFGTLHELIQEADRLGSAVVLQHVGPRQMEDFEELSMAAAL
                     AHVVTDPVPVPSQRTPLELRSTSIEGQQRRLLQAHEALLKLSDANRDQFGNLVDNLRE
                     ELEG"
     gene            8840..10009
                     /gene="aroQ"
                     /locus_tag="AC16QF_10170"
     CDS             8840..10009
                     /gene="aroQ"
                     /locus_tag="AC16QF_10170"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma subclass chorismate mutase AroQ"
                     /protein_id="HIR6448964.1"
                     /translation="MTVPVCAWAGDPGVAELAHQRLALMQDVAAHKWVNGLPVEDGAR
                     EAKVLESAEAAALRFGVDVSATRALFEAQIEAAKEIQRYWFARWALGSPPRSAPDLVG
                     DLRPRLLALGDLILAGLAAAPGRAGSVPPLPGLSESSRRRLSDALSAVEPYPSRLEQV
                     RRSGVLRIGTTGDYPPFSHRVDDDGPLGGADVDLGRLLAHSLGAEARFVPTSWPTLMD
                     DLAAGRYDVAMSGVSRTAARERVAAFTRAYHVGGKTPIARCSERERFSSLAQIDRRGV
                     RVIVNPGGTNELFVDNNLTLADKVLHTDNRSIFQALIEGRADVMITDRIEVRLQVRLH
                     EGVLCATTPRNFNHQEKAYLLPRDAAWHAYVDAWLVSMLAQGVVERTIARHLGED"
     gene            10091..10543
                     /locus_tag="AC16QF_10175"
     CDS             10091..10543
                     /locus_tag="AC16QF_10175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR6448965.1"
                     /translation="MSERQLPEGLPDWIVEHIRLYREDPDKGHMWDSTALGGPGVLPT
                     LLLTTRGRRSGEPRILPLIYAEVQGGYAIIASKGGFPTHPAWYLNLVDEPECGVQVGQ
                     DEFRARARTADGEEREAIWAQMEGIYPPYRDYQAATERRIPVVVLERI"
     gene            10591..11652
                     /locus_tag="AC16QF_10180"
     CDS             10591..11652
                     /locus_tag="AC16QF_10180"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6448966.1"
                     /translation="MTTHDESTGPEGYRVAEVEAWIAENVQALKPPFEWTRLQGGHSN
                     LTYLLVDGDGNRAVIRRPPQGELLPKAHDMSREWALISALGPTPVPVPAALGFCESPD
                     VTGAWFYVMGFVNGHPLYNSDDTRQWVPREHRRKMAFSFIDVLADLHAVDPDEVGLGE
                     LGKKENYVGRQLKTWYRSWTSSIEAADYDDPRAHELQRYLLENIPEQGPARVVHGDYG
                     LHNCLVGPDCTIAAVVDWEISTLGDPLADLAYALNWWPDPTDTEPVLPEAATAPEGFP
                     TRTELAARYAERTGRDLSKLDYYVGFNRWKSAAIVHGVYARYCEGKKTADEGVDLDEM
                     RGRIDRSLMLSEQAIARLR"
     gene            11677..12066
                     /locus_tag="AC16QF_10185"
     CDS             11677..12066
                     /locus_tag="AC16QF_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448967.1"
                     /translation="MTIFIRVLAALPALLFLVMGIQWLFAPAEAAAGLEMPLLQGVAA
                     SAQIGDLGSFFFVNGVLMALGQLPGRSHLLYAPAFLIGGAGVFRIAAALLGHAPFAVD
                     FILAEAIMTTILLVAARRLGQPAAAAA"
     gene            complement(12063..12611)
                     /locus_tag="AC16QF_10190"
     CDS             complement(12063..12611)
                     /locus_tag="AC16QF_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6448968.1"
                     /translation="MTQTNGGLPGLRGVDHIGITAPDFDQAADFLENVLGLEAFYQMG
                     PFGSEETDWMLDMLNVHPRAVIRRVKQFRCGHGPNIELFEYESPDQRREMPKNSDWGG
                     HHITLYVDDIDAAMDHLRAHGVRLMAGGGPPVDDGPEAGLRSCYFLTPWDLQMELISY
                     PDGKAYERGTDRRLWDPRDPAR"
     gene            12735..13721
                     /locus_tag="AC16QF_10195"
     CDS             12735..13721
                     /locus_tag="AC16QF_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6448969.1"
                     /translation="MRVLVTGGNRYIGRELVFELARRGHELTVINSHESPLPEGTRRI
                     HADRRLPGEFEAALSAHRDDFDAVFDNTAFTLADLEPMVELFRGRVQHYVFTSSMAVY
                     KRSFVQPLKEDFRRHAADDDDPRKAYGVGKVRCEDLLLAEWQRDAFPATWLRVGHTMG
                     PRSPLPTRDPAFFARLEAGRPILVPGEGFAVLQLVHINDVARCMASLLGNERVRGEGY
                     NVSGSEVASVLGVVHLIGRAMGVQPRVVNVPMEVARAHRPPLLHWGESVSGSAMLSID
                     KALAHLDWEPRFGIEAGYRDAYEWWQREGRGLFEFDFAPEDALVERLGLATE"
     gene            13734..14048
                     /locus_tag="AC16QF_10200"
     CDS             13734..14048
                     /locus_tag="AC16QF_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448970.1"
                     /translation="MTIKSAIYDLLVSRRYDAELETVTEDARRRCVEMLAPRPGGRCA
                     MMDTYWPKPPLRLWLMSWSYAADPKRPGFELLRATCDDFSLEHFPPDVEHGFYLASGT
                     KR"
     gene            14117..14926
                     /locus_tag="AC16QF_10205"
     CDS             14117..14926
                     /locus_tag="AC16QF_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HIR6448971.1"
                     /translation="MKRVLVFLLIGFSTPAFAGPRLYVFDCGLLSLDSVEIFNLAEDE
                     TSVRELFVPCYLIEHENGRMLWDGGLPLSYAQAEGPVAIDGGSVSYERSVVDQLADMD
                     ITPEDVDLVAYSHLHWDHAGTANTFIASEVLMQRAEWAAAMGEGGEFVDTSLFDKLVD
                     AEVTFLEGDHDVFGDGSVRIIFTPGHTPGHQTLLISLEKTGPVMLSGDLYHFRANRRL
                     RRPPNFNVDQEQTLQSMDRLEAVLEETGATLWIEHDKALAETLRKAPAFYD"
     gene            complement(14986..15450)
                     /locus_tag="AC16QF_10210"
     CDS             complement(14986..15450)
                     /locus_tag="AC16QF_10210"
                     /inference="COORDINATES: protein motif:HMM:NF017771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HIR6448972.1"
                     /translation="MSSDRIETLERQIFDLTRELHELRKANVGDEVPNYTFATLTGEA
                     SLLDLFGSRDTLLAIHNMGQGCRYCTLWADGLNSLLPHLESAMAVVVLSKDAPETQRL
                     FANSRGWQFRLASHGGGNYMAGRSVLLDLERVCARRAGRIELDAPVQLLAAA"
     gene            complement(15473..16837)
                     /locus_tag="AC16QF_10215"
     CDS             complement(15473..16837)
                     /locus_tag="AC16QF_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6448973.1"
                     /translation="MSQKRLSRSTLLYFGLPDYAIYLAAIPVSLYLPFVYSRDLGLDL
                     TDIGLILMLARISDVITDPLIGYLSDRTQTRFGKRKPWLAGGALVMMVSAWQLFAPMG
                     PVTNWHLFIWSMLLWLGWTMINIPYFAWGAELSDDYNERTRITGWRQAFGYLGNVSVL
                     AIPTLSGELFGYGSLPREGLTIVGFMALVLLPVLMALALWKVPEPEKHRATEANLLVS
                     SRQMFSNGSFMLLFTGFLMLSMGTTWASSMFMLFATFVVDVEGYTQAILLGFYFVQLL
                     SLPLWLQISYRIGKKETWLLGAAIYAIASPGYLMLGSGDVVGLGIVLGLYGIASGNFT
                     AISLSMKADVIDVAALRTGNHVAGSYMAVWSLGQKLFQAIAVGLALPAVAYFGFDPKG
                     ENGPDELLALRLNITVVPMLLYAASVFVIWRYPLTADRLHQLREAFSRRRARREIKQG
                     STGA"
     gene            complement(16901..17104)
                     /locus_tag="AC16QF_10220"
     CDS             complement(16901..17104)
                     /locus_tag="AC16QF_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448974.1"
                     /translation="MSAGTNQLAIEYKGRQYSGVYSVSGNLMIARIPGISSRSLETAE
                     ADESQARTLLMEILKEADTLGQL"
     gene            17268..17873
                     /locus_tag="AC16QF_10225"
     CDS             17268..17873
                     /locus_tag="AC16QF_10225"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR6448975.1"
                     /translation="MAGDARATIALDVSESIWERFFCVFPLVVVGTVEPDGTYDLAPK
                     HLAMPMSWKNHFGFVCTPRHRTYQNIERTGEFTVTYARPSQAVLSSLAATPRCEDGSK
                     PITAALPTRPAQTVAGVFLDDGYLFLECRRYRVYDDFGTNSLITGEIVAAGVAEDALR
                     SADEDDQDVIRRAPLLSFLYPGRIAEISASQQLPLPAGFSR"
     gene            17878..19050
                     /locus_tag="AC16QF_10230"
     CDS             17878..19050
                     /locus_tag="AC16QF_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR6448976.1"
                     /translation="MSRADDIRRHLQAEEPEMLELLTALVTLESPSDVPETQGPVLDE
                     LQRVFDGLGYRTIRVPGRASGGHLYARPGRRRSGGNRRWQLLLGHCDTVWPVGTLEKM
                     PLAADGNHLKGPGIYDMKAGLVEMVFALKALEALGLEPEVEPVIFINSDEEIGSREST
                     RYIRALSQRADRALVMEPSLGPRGRLKTARKGVGRFTVTVRGKAAHAGLDPTAGASAI
                     LELSHVIQKLFALNDPERGASVNVGVIDGGVRANVVAPESSAVVDVRVASQEDAERIE
                     RAILSLEAETPGVSLGVVGRIGRPPLEHTPANRQLWRVATDLACELGIQLEEAMAGGG
                     SDGNTTSLFTATLDGLGAVGDGAHADHEHIQVDQLSQRCALLAMLLLAPPLNSGTG"
     gene            19061..20062
                     /locus_tag="AC16QF_10235"
     CDS             19061..20062
                     /locus_tag="AC16QF_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6448977.1"
                     /translation="MRHLLTSVTRICDLAEGAFDVAPVPKDTWATGDYVVGEVLDASG
                     YHNVELPNGRLMEAMEGDRVVGAFGARAATLEAVGDWQSIQGDEFDALTPAGLFGRLT
                     SVSPFLPRLMGMRYLGHVHANGVRQTMDGAVTPAPDAALTVPIVLITGTSMSSGKTMS
                     GRLIVRLLSRLGFRVVGAKLTGAARYRDVLSYEDAGAAAVFDFVDEGLPSTVGDPADF
                     AARLERLLSRIAGAAPDVVVAEAGASPLEPYNGKTAIDVLGDRVRFNLLCASDPYAVV
                     GVASAFERTPDLVAGGAANTTAGIELVSKLSGLTAMNLLGKDCHEPLTRMLRDKLGL"
     gene            complement(20067..20918)
                     /locus_tag="AC16QF_10240"
     CDS             complement(20067..20918)
                     /locus_tag="AC16QF_10240"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR6448978.1"
                     /translation="MNVELELLASGYRLLEGPRTDADGALWFSDVYGGTVNRRDPDGI
                     IEVVVRDRRMVGGLLMHANGGFVMTGPSVVLWRDGQERVLLERDGVRFFNDLHADASG
                     RIYVGSIRADIANLDDEPDPPGEAYRIDLDGSVTELYGGVGISNGIGFSPDGRTLYHV
                     DSTSRGIWAHDLDDAGALSNRRHIAKPAFEKGIPDGLCVDTEGNLWVAHVQGGRVVVL
                     DPGGNHVDEIRVPASWVTSCAFGGSDYRDLYIVSADNTDDKALGGCVWRCRPGAAGHP
                     TPLARVG"
     gene            complement(20936..21436)
                     /locus_tag="AC16QF_10245"
     CDS             complement(20936..21436)
                     /locus_tag="AC16QF_10245"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6448979.1"
                     /translation="MAYTPEQLSDLECIRDAAKRYCRGVDRLDEALMKSAYWPEATDD
                     HGVFVGNAMEFAEMCMVSHLRWRSTSHCIFNHSIELDPDGIHARGEAYNVTFLFQDGA
                     DVLDTWYGRYLDVYEKRGDEWRILERVCVHEGTTTGPVSKMEIDAEKFRHGSFDRPSS
                     GRRIGP"
     gene            21566..>22971
                     /locus_tag="AC16QF_10250"
     CDS             21566..>22971
                     /locus_tag="AC16QF_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6448980.1"
                     /translation="MVDDSGTFPTAGVLGRAFRSGELSPVAVTRELLERIDRLNPALG
                     AVHRLCPERAMAEAAAAERAFSAGEARSPLQGVPYLVKDLFDVAGLPTTAGTRLLEDN
                     VAEADSTAVARLAAAGMVLLGKTVTVQFAYGGAGVNHDHGTPRNPWRQDHYLPGGSSS
                     GSAVAVAAGLAPLALGSDTGGSVRIPASLCGITGLKTTVGRISRAGVYPLSWSLDSVG
                     PLARSVEDAARVYQLLAGADPGDETTLGRPAGDVLAGLEDGLDGLKIGIPENVFWEEV
                     DAEVAEAVLASADVLRSLGARVERMAFPAADGARLLNPRGLVIAAEAYAVNRRFMDDH
                     YDELDPVVATRMIQGKGVAAADYIENLLQWQALRVEAMKQMAGVDALICPSTMIPATP
                     LADCDCDLDRYVERNLAYLRNTSIGNVLNLCGLSVPCGFTAAGLPIGAMICGSPFAEA
                     TVLRIGHAYQRATDWHQRV"
BASE COUNT         3765 a         7506 c         7665 g         4035 t
ORIGIN      
        1 cagcccgacg tgcccgcgca tcacatcgaa ggcagcaggt acacggaccg cggctttgcc
       61 gctgcggagt gggacgggat gtggagcagg gtctggctgc tgcttggccg ggcccgggag
      121 atgccgaacc cgggcgacta ccaggtggag gacgtcgggc ccgagtcctt catcatggtg
      181 cgccaggacg acgggtccat ccgcgcgttc tacaacgtct gccagcaccg gggctcgcgg
      241 ctgatcttca atgccgccgg ttccgtggag cgcatctcct gcccttacca ctcgtggacc
      301 tacggccgtg acggcgcgct gctggtcgtg caggaccctg aggatttcag ctccgggaat
      361 ccctgcgagc ggctgagcct ggtggaggtg cgctgcgaga ccttcgccgg ctgggtgtgg
      421 gtcaacatgg accccgactg cgtctcgctg cgcgaatacc tcggtcccgt gtgggaagac
      481 tggcagacgt ggcaacccga ggactggcgc cggtccctgg ccatctccgc ttgggtaccc
      541 tgcaactgga aggtcataca ggacaacttc tgcgagtcct accacctgct gaccgtgcac
      601 ccgcagctca cggcgaacat cgaggaaggc gtgccgtgga cgcgcttcga catggccaac
      661 gaaggccaca accgcatgat catgcaggcg ggcatgccgt cccatcgcca ggcccagggg
      721 ccaaagctcg tggaaccgct gctctcccag atggccatgt gggagctcga ccccgacgac
      781 ttcgccggcc gggagcatga tacccgggcg gcgatccagc agcagatgcg cgatctcggc
      841 gctccgcggg gctatgctca ctacgaccgc ctgcgcgacg agcagctcac cgatccgcac
      901 cactacaaca tcttccccaa ctgctcggtg accttcggtg ccgactctgt gctgctgcag
      961 cggatgcggc ctcatcccac cgatccgcag cagtgccgtt tcgatcactg gacctacgtg
     1021 gcaccagccg ccatcgagca cggggtgttc cgcaactcag acggccgggt cgaattcacg
     1081 ggcgacgccg agataaagga agttgagtac gggaaggaat ccatgggtcc catcgccgat
     1141 caggacatcg gcattaccac cgggcaacag ctcgggctgc gctcgcgcgg ttaccggggc
     1201 gccaatctgg cagaccagga gtcgcgcatc gcacggttcc atggggtgat cgacgagtac
     1261 atcgccggcg agcggccttg agcggcgatc gggaaacggc tgccaatccg gcttcggtga
     1321 gcggaagaga gcgacatggg tgactacgtt tacccgggca ttctgagcag cctggcgctg
     1381 ctcgtttact acttcaccct gttcaaggcc ggcatggccc ggggcaaata caacataccg
     1441 gcgccgtctc atgagggccc tgaggagtac gtccgcaggg ttcgcgtgca tcagaacacc
     1501 atggagcacc tggtgctgtt tctgcccggg ctgtggctgt tctcctacgc cgtggacccc
     1561 atctgggcgg cggggatcgg ggttctttgg cccgtcggcc ggctctggta tgcgtttgcc
     1621 tactacgagg cggccgagaa gcgtgggccc gggctttatc tcagcatgcc ccccatctac
     1681 atattcgtga tcggatcgct catcgggttt atgctggagc tgttttagac cgcgggagcg
     1741 agcatgcccc gattcagtat tctggacctg gcccccatca ccattggcgg atccgcacgc
     1801 caggcgctgc acaacgcgcg cgacctggcg cgtcatgggg aagcctgggg attcgagcgg
     1861 ttctggctgg ccgagcacca caacatgccc ggcattgcca gctcggccac cgccgtcgcc
     1921 atcggtttcg tggccgaagg cacgtccacg atccgcgtgg gagccggcgg catcatgctg
     1981 ccgaaccacg cgccgctggt gatcgccgag cagttcggga ccctggaggc cctttacccg
     2041 ggccggatcg acctgggtct cggccgggcg cctggcacgg atcagatcac ggctcacgcg
     2101 ctcaggcgga cgctcaacag cgacgtggac gggttccccc aggatctcat ggagctcatg
     2161 ctctatctgg gcgatccggt cgagggtcag cgcgttcatg cggtgcccgg ggcggggtcc
     2221 aaggtgccgg tatggatctt aggctcgagc ctgttcggtg cggaggtcgc cgcagccctt
     2281 gggctccctt atgcgtttgc ctctcacttc gcccccgcgg ccatggatca ggccatcgag
     2341 gtctaccgga agaacttcaa gccctcggag catctggatg ctccctacgt catgctcggt
     2401 ttcaacgtct gcgctgcgga cacggaggag gaagccgttt acctgcgcag ctcgtccctg
     2461 caggcaatcc tccagctgcg ccagggtgcg ccggggcggc tgccgccgcc ggtgcacgac
     2521 ttcgacgcca ccctcacgcc ggagcagcgt tacctcatcg atcggttttc cctgtgctcg
     2581 gcggtgggca cacgggagac ggtagagcgt tccatcaacg acttcctcgc ccggacgggt
     2641 gccgacgagc tcatcatcgt cggccagatc ttcgatcacc aggcccggct gcggtcgttc
     2701 gagatcgttg cggagctgat cggagactta cagcccgggg actccacgcg ggttgccggg
     2761 cagcatggat gacgagctcg acctgatggg gcccgaggag atggccgacc cggccgtctc
     2821 cgcccggcga ctgctcgaag gcccgcgctg ccggctctac cacgggttcg acccgccgtt
     2881 ctacgtcgtc tcccgctacg acgacgtgag cgccattgtg agggatccag ggcgttttct
     2941 gagcggccac ggtcagggtc ccaacttcgt cgcgcccatg ggcgtggtgt ccgacccgcc
     3001 tcagcacacc tttttccgcg agctggtcca gcctgatttc ctgccaagat ccattgccct
     3061 gctacgacct cgcctggaag ccatcgccga cgagctgctc gacgcggtcg cggaagagac
     3121 ggtctgggac gtccacgacc ggctttcctt tccgctgccg gtgaccatca tctgcgagat
     3181 attcggcata cccacggatg acatcgagca gttcaagatc tggtctgacg cctccgtcgc
     3241 cgctctgtcc tcccagaacc cgcagcagta cgacgcggag cttgcgcgca tgcaggccta
     3301 cgtgctggaa cggctgcgcg ggacacggcg ccgtcctggc gacggcagcc tgatctcgcg
     3361 catcgcccgt gcccgccgag acggcgtgcc ggtgacggac gaggaagccg ttcgcctggt
     3421 gctgcagctc ttcgtcgcgg gaaacgagac cacgacctcg ctcatcacca atttcgtctg
     3481 gcgcatgctg gcaggcgacg ggctttgggc cgatttctgc gcgggccgcg tcgacgtgga
     3541 ccgggcgatc aacgagagcc tcagattcga tccgcccctt ctcggactct tccgcaccac
     3601 ggccgtggac gtcgatgtcg ggggaaccgt gatacccgct cactccaagg tgctcaccca
     3661 ctacggcgct gccaatcggg acccggcggc gttccgggat ccggacgtat tcgacgtggg
     3721 ccggactggc cggaaggttc tttcctttgg gctcggcatc cacgtctgcc tgggccgcga
     3781 gctggctttg ctcgaggctc gggtggccct gcaaggtctc gcgcggcggt ttccgcgcct
     3841 cgagctggtg gacgaaggcg aacggattgg cccgtttctc ttctggggtc gcaggcacct
     3901 gccggtccgt aatcgagcct gacggccatt ccctaagcgc ctggacctcg cctacaatga
     3961 agtcatgtgc caatcccacg acgtagccaa cgagaccgaa aacgccgatc cacgcagcgg
     4021 ttaccgcctg gcggaaggtt ggccccatta cccggccgac atgcagttcg agatgggctc
     4081 cggggtggcg gtggatgagg ccggggtgat ctatctgttc actcgggaca tcgagcactg
     4141 ggccgctcat cccctggcca tgaaggacaa gatgggcaag agcagcatct ccatgttcga
     4201 tcgggccgga aactacctcg gcaagtgggg cccctcggac gagcgcggct tcgcgcttgg
     4261 cgcccatacg ctctacatcg agaaggacgg catgttctgg accacggacc gtgacggcca
     4321 cacggtgcgc aaatatcatc ccgatggaac cctgctgctg accctgggcg agttcggcca
     4381 gtggggtgcg gggaccgacc atttcaacgg ccccacagcc gtgctcgtgc aggaaaacgg
     4441 caacatcgtg gtgtcggacg gctactggaa ctcccggctg gtgtggtttt cgcccgaggg
     4501 cgagttcatc cgcgaggtgg gtacctgggg gactgggccg gcggagttcg gcacgcctca
     4561 cgccatcgct caggacgccc agggtcggat actggtggcc gacctcgccg gcggcaactt
     4621 tcatccgtat atgaccctgc cgggccagat cgccgagcat cggacccggc agattccgga
     4681 tgccaaaagc cgtattcaga tcctcgaccc ggacggcgcg tacatcggcg agtgggatca
     4741 catcatgccg ctgagcctcg ccgtctacgg cgatcacgtc tacgccagcg acaagatgag
     4801 caaccttgcg attctggatg cggccaccgg caaggtggaa gagcgtgtcg acaacatcgc
     4861 catctacatc catcagctgg cgatggacgc ccacggtgat ctctacacgg catccgtcta
     4921 cccggagcac gccggcgaga agcgcggccc cgagggtccg tcccaccggc gctggacccg
     4981 cgacggcgcc tgatcccgaa ggaatctgat cctttcctgc gcggaccgcc cgtccgcccc
     5041 gccccctggt tgaccggctg cccttcatgt cccgttcagg ttgacgggga tagtctcgtc
     5101 cgatatgagt ggaaagaacg ccagggactg cctcgccacg cggatcagga agctgagagg
     5161 catcggatga ttacagggtt ccattgctca acgtcctggg tccggcagcg ggcccgggtg
     5221 gtcgccttgc tcgggttgct caccctgctc gtggcaggca acacggcccg tggggagccg
     5281 gccatggaag ccgtggccgc ggacggaagc acctacccgg tggagccggt agcgctcctg
     5341 gatccggcgg ccctggagga actggtcggg cccatcgccc tctatcccga cgaccttctg
     5401 gctattgccc tgcccgccgc gacctatccg ctgcaggtcg tgcaggcttc tcggtttctg
     5461 caatcgcgcg aggagaaccc ttccgtgcag ccggctgagg ggtgggacga ctcggtggtg
     5521 gcgctcctca actaccccga ggtggtggag ctgctgaacg aagatctgga ttggacctgg
     5581 aagctcggcg aggccgtgct cgcccagcag gaggatgtgc tcgaggcggt cgcggcgttt
     5641 cgggaacgcg cccggcttgc cggcaacctg cgcagcgacg acttccaact ggtggagagc
     5701 gcggacgagg gtatccggat ccggccgcgg gatcccgagg tgatctacgt tccctactac
     5761 gaaccttcca gggtgaccgc ctaccactcc cgcccggtgt atcactacta cccggctcgt
     5821 tatcccgttt actactaccc ctatgcgccc ggacatcgat ttgcgagcgg gttctggggc
     5881 gtgacctcgg cattcagcat cggctggacc accaggcggc ttcacgtgca ttacgtgacc
     5941 taccgggacc atccctacta cggccatcat tattacgatc gatacttcta ccgtcggcca
     6001 ccgtccttcc gggtcgacga ccactggcag tggcacggca gctttgccgg gctgggacgc
     6061 catcatgcag gcaactactg gtatccgcgt gcacatcgtc acggcccgag cccgcggcac
     6121 catcaccgac gaagccatca cgaacggaac caggatgagg ggacggatcg cgagccccga
     6181 cgacatcggg agcgggagcg ggaaagagcc gctgaccgcc ccgggggttc cgaggagcac
     6241 ctcgccgacc ggcgcgaccc gcgggaacgg ctcgtcccgg agcttggacc ccgccgcgct
     6301 ggccgtctgt tggcgagggg atcggtggtg aacggcattc cggtgcccga gtcggcggcc
     6361 acgctggcgc cgcgacccgc gcggccgccc gcggctgccg attccccggg gtcggcccag
     6421 cgccggcccg gctcacccat tgaggcgcag caggcgatcg cgcgtgcccg ttcggctctc
     6481 ggcgccagaa gcgccgccag gggcggggcg ttgagagcgc agaggtcggc cggatctccc
     6541 cgcgctccgg cccgggctat ggccgggccg acaccggggc agcagagcac gacggcctcc
     6601 ccttcgcggt ccgcggtagc tgtgccctcg cggccggctc cgatggcgtt catgcctcga
     6661 tcttcgccgc cgcggccggc ggttcccacg tcccggcgct tgcagcctgc cccgtcggcg
     6721 cgcacctccc ggccttctca gcccgctccg tcggcccgca cgtcccggcc ggccttcgat
     6781 cccgggacca tttccaggcg gggcggcggg gttcgcgccg gcaggccgac gcccatgcgg
     6841 ctcgactgag cgcgccagat agcgtttgcg gcttcgcctg gcagacttaa aggcgtaatc
     6901 tgtacgtcat gaagaaggct gcgcttcctg agaacgaggg cgaacgtctt gagcggctgc
     6961 gtgactacga tattctcgac acgcttcccg agcaggacta cgacgacatc acctatctcg
     7021 cttcgcagat ctgcgatacc cccatagccg tcatcagcct ggtggatgac gatcgtcagt
     7081 ggttcaagtc ggcccagggc ttggatgtct ccgagacacc gagagacgtt gcgttctgcg
     7141 cacacacgat tctcgagccc gacaaactca tggtggtgga ggacgccagc caggatgacc
     7201 gctttgcgga caaccctctg gtcgccggcg atcttggcat acgcttctat gccggcgccc
     7261 ccctcgtcac cgcgtcgggt cacgccctgg gcacgttgtg cgtcatcgac cagaaacccc
     7321 ggggactgac cgatcagcag cgcaaaacgc tcgcggtgct cgctcgccag gtggtcgcgc
     7381 tcctcgaatt gcggcgttcc gtcgccgagc ttaagcgtag ctcggatgcc cttcacgagt
     7441 ctcaccagtt tctcgccctt cgggggcagc agctcaacag gtcgcgggac gagcttgctg
     7501 aagccgtgcg ggtgctggag ggtcaggcgg acgtgatcga gcaggatctg catcgggcag
     7561 aagtgatcca gcgttcgctg ctcccccacg aggtcccccg gctcgaggac ttcagcgtgc
     7621 gatcgcttta tcgcccgggg cacacggtgg gcggggatct gtacgacgtg gtgtcaatcg
     7681 cggaccgtta tctcgccatc gtggtcgccg acgcctgcgg tcacggtgtc agcgccgcga
     7741 tgctctcggt ccttttcaag cagcacctgc ggctgcagga cccggcgtcg ggcattcctt
     7801 acccgccggc gtgggcgctg gagcgcatca accgttccgt ggtcgccaac cggcctgcgc
     7861 ccggcgtgtt tctgaccgcc gtctacgcgc ttgtggatac tcagacccga aagctcaagc
     7921 tgggctccgc cggtcatccg ccggtaattt gtctgcgcgc gagcggcgag gtcgagtcgt
     7981 ttgcccacac gggcccggcg ttggggctcg acgagagttc gacttacgag gaggccgaaa
     8041 tcgagctggg cgtgggcgac aagcttctgt tctacaccga cgggctgctg gacatctgcg
     8101 aatcttcccc cacggtggaa gcgctcggcc aggccatggt ctcggtagcc gacaagccga
     8161 acctcctcga gcaggtgctc atggaggtga ctcacggccg cgcccgcccg gattgcgacg
     8221 acgtgaccat ggtgctgctg gatgccgctc ccggcgaaag cgtgttcaac gagtctgtgg
     8281 agggactcga gctgcaggag atgccgacca cggcctcgcc gacgatcagc tgggcagcct
     8341 cggaggacgg ggtctttctc atcctggagg gctgggtgac ctggctgtgc ggggagacgt
     8401 tgttcaatgc ggccatgccg ttgatcaaag cgcaccggcg ccttgtcatc gacctcgagc
     8461 tttgcgagca catggacagc accatgttcg gcaccctgca cgagctgatc caggaagccg
     8521 accggctcgg atccgcggtg gtcctgcagc atgtgggccc gcggcagatg gaagatttcg
     8581 aggagctcag catggccgct gcgcttgccc acgtggtcac cgacccggtc ccggtgccat
     8641 cccagcgcac gccgctggag ctgcgcagca ccagcatcga gggtcagcag cgacgtctgc
     8701 tccaggctca cgaggcgctg ctcaagctca gcgatgcgaa ccgcgaccag ttcggcaacc
     8761 tggtggacaa cctgcgcgag gagctcgaag gctgacgctt cccgggttaa gtcggcgatg
     8821 gtggtgcgtc gccctgtgtc tgactgttcc tgtatgtgcg tgggctggcg accccggggt
     8881 cgccgagctt gcccatcagc gccttgcgct catgcaggac gtggcggctc acaagtgggt
     8941 taacggcctc ccggtcgagg atggcgcgcg cgaggcgaag gtgctcgaaa gcgcggaagc
     9001 tgctgcgctt cgattcggcg tcgacgtgtc tgccacccgg gcgctcttcg aagctcagat
     9061 cgaagcggcc aaggagatcc aacgctactg gtttgctcgc tgggcccttg gctcaccgcc
     9121 gcgctcggcc cccgacctcg tcggggatct ccggccccgg ctgcttgccc tcggggacct
     9181 catcctggcc gggctcgccg cggcgccggg tcgcgcaggg tcggttccgc cgctcccggg
     9241 gctcagcgaa tcgtcgcgtc ggcgtctgag cgatgcgctg tcggccgtgg agccctatcc
     9301 gagccgtctg gagcaggttc gccgctcggg cgttctgcgc atcggcacca ctggagacta
     9361 ccccccgttt tcgcaccgtg ttgacgatga cgggccctta ggcggggcgg acgtcgatct
     9421 cggtcgcttg ctggcccatt cgctcggagc ggaagccaga ttcgtcccta cctcctggcc
     9481 taccctcatg gacgatctgg ctgctggacg ctacgacgtc gccatgagcg gggtgtctcg
     9541 gacggcggcg cgcgagcggg ttgccgcgtt tacccgcgcg taccacgtcg gcgggaagac
     9601 gcctattgcc aggtgctcgg agcgggaacg gttctcgagc ctcgcccaga tcgaccgccg
     9661 cggggttcgg gtcatcgtca accccggggg caccaacgag ctctttgtgg acaacaacct
     9721 gacgctggct gacaaggttc tgcacacaga caaccgctct atctttcagg ctctgataga
     9781 aggccgtgcg gatgtcatga tcaccgaccg tatcgaggtg cgcctgcagg tccgcctgca
     9841 cgaaggggtt ctttgcgcca ccacgccccg taacttcaac caccaggaga aagcctacct
     9901 cctgccccgg gatgcggcat ggcacgccta cgttgacgcc tggctcgtca gcatgctcgc
     9961 tcagggcgtg gtggagcgca ccatcgcccg gcatctgggc gaggattgac gcgagggcga
    10021 cggctggcgg ggacggcttc tctggtgcac catgagagca tcgagccgac tgaaagggga
    10081 ggaagcggac atgagtgaac ggcagctgcc ggaaggcttg cccgattgga tcgtcgagca
    10141 catcaggctg taccgggaag atccggacaa gggccacatg tgggattcca ccgcgctggg
    10201 cggtccgggc gtgctgccca cgctgctgct gactacccgt gggcgcaggt cgggggagcc
    10261 gcgcatcctc cccctcatct acgcagaggt gcagggcggc tacgctatca tcgcctccaa
    10321 aggcgggttt ccgacgcacc cggcgtggta tctgaatctc gtggacgaac cggaatgcgg
    10381 cgttcaggta ggccaagacg agttccgcgc ccgggcccgc accgccgatg gcgaggaacg
    10441 ggaagccatc tgggcgcaga tggaagggat ctatccgccc taccgggatt accaggccgc
    10501 caccgagcgg cggataccgg tggtggtgct ggaaaggatc tgaggggccg gaaggcgcgg
    10561 gggcgccgcc gtcgcgcggg gggagatacg atgacgacgc atgatgagag cacgggtccg
    10621 gagggctacc gggttgccga ggtggaggcg tggatcgccg agaacgtgca ggccctgaag
    10681 ccgcctttcg agtggacgcg ccttcagggc ggccactcca acctcaccta cctgctcgtg
    10741 gacggtgacg gcaaccgggc cgtcatccgc aggccgcccc agggcgagct gctgccgaaa
    10801 gcccatgaca tgagccgcga gtgggccctg atctcggccc ttgggccaac accggtgccc
    10861 gtgcccgccg cgctgggatt ctgcgagagc ccggacgtca ccggcgcctg gttctacgtc
    10921 atgggcttcg tgaacgggca cccgctgtac aactccgatg acacccggca atgggtaccc
    10981 cgggagcacc gtcggaagat ggcgttctcg ttcatcgacg tgctcgccga tctgcacgcc
    11041 gtggaccccg acgaggtggg cctgggagaa ctggggaaga aggagaacta cgtgggccgt
    11101 cagctcaaga cctggtatcg ctcctggacc tcgtccatcg aggcggccga ctacgacgac
    11161 ccgcgcgccc acgagctgca gcgctacctc ctggagaaca ttcccgagca ggggccggcc
    11221 cgcgtggttc acggcgatta cggcctgcac aattgcctgg ttggtccgga ttgcaccatc
    11281 gccgcggtgg tggattggga gatatctacc ctgggcgatc cgctggcgga tctggcctac
    11341 gcgctgaact ggtggccaga cccgaccgac acggaacccg tgttgcccga agcggccacg
    11401 gcccccgaag gattcccgac ccgcaccgag cttgcggcac gctacgccga gcgcaccgga
    11461 cgcgacctct ccaagctcga ctactacgtt ggcttcaatc gctggaaatc ggccgccatc
    11521 gtgcacggcg tttatgcgcg ctattgcgag gggaagaaga cggcggacga gggggtggat
    11581 ctggacgaga tgcgaggtcg aatcgaccgg tcgctcatgc tttcagagca ggccatcgcg
    11641 cggctgcgct gaacggccaa tgctagggag acgatcatga cgatattcat ccgggtgctc
    11701 gccgcgttgc ccgcgctgct gttcctggtc atgggcattc agtggctgtt tgcgccggcg
    11761 gaagctgccg cgggtcttga aatgccgctg ctgcagggcg tggcggcaag tgcgcagatc
    11821 ggcgatctgg ggagcttttt cttcgtcaac ggggttctga tggctctagg tcagctcccc
    11881 ggccggagcc atctgctcta cgcccccgcg tttctcatcg gcggggcggg cgtgtttcgc
    11941 attgccgccg ccctgctggg tcatgcgccc ttcgcggtgg atttcattct cgccgaagcg
    12001 atcatgacga cgattctgct ggtggccgcc cgacgactgg gccaaccggc cgctgcggcg
    12061 gcctagcggg ccgggtcccg aggatcccac aggcgacggt cggtgccccg ctcgtaggct
    12121 ttgccgtcgg ggtaggagat cagctccatc tgcaggtccc agggggtcag gaagtagcag
    12181 gaacgcaggc ccgcctcggg gccgtcgtcc accggcggcc cgccaccggc catcaggcgc
    12241 acgccgtggg ctcggaggtg gtccatggcc gcgtcgatgt cgtccacgta cagcgtgatg
    12301 tgatgcccgc cccagtcgct gttcttcggc atctcgcgcc gctggtccgg agactcgtac
    12361 tcgaaaagct cgatgttggg tccgtggcca cagcggaact gctttacccg gcggatcacg
    12421 gcccgcgggt ggacgttgag catgtcgagc atccagtcgg tctcctcgga gccgaagggg
    12481 cccatctggt agaaggcctc caggccgaga acgttctcca ggaagtccgc cgcctgatcg
    12541 aagtccggcg ccgtgatgcc gatgtgatca acgccgcgca gtcccggcag gcctccgttt
    12601 gtctgggtca tgacatgctc ccctttgctc ccgttgccga caccgccaag gttagcagca
    12661 acgcccgggg gtgtgcatgg cgtgctgtgg ccggcctctg ctatcttcag cccgaacgca
    12721 ggggagggtc ctagatgaga gttctggtca cgggcggcaa ccgttacata ggccgcgaac
    12781 tcgtcttcga gctggcccgc cgtggtcacg agctgaccgt catcaacagc cacgagtccc
    12841 cgctgccgga aggaacccgc cgcattcatg cggaccgccg gttgcccggg gagttcgagg
    12901 ccgctctgtc tgcccatagg gatgattttg acgccgtgtt cgacaacacc gcgttcacgc
    12961 ttgccgatct cgagcccatg gtcgagctct tccgcggtcg ggttcaacac tacgtcttca
    13021 ccagctccat ggccgtgtac aagcgcagct tcgtccagcc gctcaaggag gacttccgtc
    13081 ggcacgcggc agacgacgac gatccccgaa aggcttacgg ggtcggcaag gtcaggtgcg
    13141 aggacctgct gctcgccgag tggcagcgcg acgccttccc cgcgacgtgg ctgcgcgtcg
    13201 gccataccat ggggccgcgc agcccgctgc ccacgaggga ccctgcgttc tttgcgcgtc
    13261 tggaagccgg acgcccgatc ctcgtacccg gcgaggggtt cgccgtcctg cagctcgtgc
    13321 acatcaacga cgtggcgcgc tgcatggcgt ccctgctggg caacgagcgg gtcaggggtg
    13381 agggctacaa cgtatccggg agcgaggtgg ccagcgtgct cggcgttgtg cacctgatcg
    13441 gacgggccat gggcgtgcag ccgcgtgtcg tcaacgtgcc catggaggtg gcgcgcgctc
    13501 accgtccgcc gctccttcac tggggcgagt cggtgtccgg atcggccatg ctctccatcg
    13561 acaaggccct cgctcacctc gactgggagc cccggttcgg catcgaggcc ggataccgcg
    13621 atgcttacga gtggtggcaa cgggaaggcc gcggtctgtt cgagttcgac tttgccccgg
    13681 aagacgcgtt ggtcgagcgc ctcggcctgg caactgagtg agacgggatc agcgtgacca
    13741 tcaagtcggc catctacgat ctgctcgtct cccggcgcta cgacgcggag ctggagacgg
    13801 tgaccgagga tgcgcgccgc cgctgcgtgg agatgctcgc gccacgcccc ggcgggcgtt
    13861 gcgcgatgat ggacacctac tggccgaagc ctcctctcag actctggctc atgtcctgga
    13921 gctacgcggc ggatccgaaa cggccgggct tcgagctcct ccgcgcgacc tgcgacgatt
    13981 tctctctcga gcattttccg cccgacgtcg agcacggctt ttacttggcc agtggcacca
    14041 agcgctgact ccggcccatg gcaccggctg ctctgatggt atggttgtcg ctcccgttcc
    14101 tgagactcgt cccaaagtga agagagtgct cgtctttctg ctgattggtt tctcgacgcc
    14161 ggctttcgct ggtccgcggc tctacgtctt cgactgcggg ttacttagcc tcgacagcgt
    14221 ggagatcttc aacctcgccg aggacgaaac gtccgtgcgc gaactgttcg tgccgtgcta
    14281 cctcattgag catgagaacg ggcgtatgct ctgggacggc ggcttgccgc tgtcctacgc
    14341 ccaggccgag gggcccgtcg ccatcgatgg gggatcggtg agctacgaac gatccgtagt
    14401 cgatcagctg gcggacatgg acatcacccc ggaggacgtc gacctcgtcg cctactcgca
    14461 cctgcattgg gaccatgcgg ggacggccaa caccttcatt gcctcggagg tcctgatgca
    14521 gcgggccgag tgggcggcgg ccatgggtga gggcggcgag ttcgtcgaca cttcgctctt
    14581 cgacaagctg gtcgatgccg aggtcacgtt tctcgagggc gaccacgacg tgttcggcga
    14641 cggcagcgtc aggatcatct tcacgccggg ccacacgccg ggccatcaga cgctgctcat
    14701 ctctttggag aagacgggtc cggtgatgct ttctggcgat ctgtatcact ttcgtgccaa
    14761 caggcgcctg cggcggccgc cgaacttcaa cgtcgaccag gagcagacgc tccagtccat
    14821 ggaccggctc gaggcagtcc tcgaggagac cggtgccacg ctttggatcg agcacgataa
    14881 agccctggcg gagacccttc gcaaagcgcc cgccttctac gattgagccc gcgccgggtg
    14941 gcgccggttc agtccaggat gttcgcgccg ccgtcgtcga gctgctcagg ccgccgccag
    15001 tagctgaact ggggcgtcca gctcgattcg tccagcccgg cgagcgcaaa cacgttccag
    15061 atcgagcagt acagatcgcc cggccatgta gtttccgcct ccatgggaag caagacggaa
    15121 ctgccagccc cgtgaattgg cgaaaaggcg ctgggtttcc ggcgcgtcct tggacagcac
    15181 caccaccgcc atggccgact ccaggtgtgg cagaaggctg ttcagcccgt ccgcccagag
    15241 ggtgcaatag cggcatccct ggcccatgtt gtggatggcg agcagcgtgt cccgggaccc
    15301 gaaaagatcc agcagactcg cctcgccggt cagcgtggcg aacgtgtagt tgggcacctc
    15361 gtcgcccacg ttcgccttgc gcaattcgtg aagctctcga gtcaggtcga agatctgacg
    15421 ttccaacgtc tctattcgat cactgctcat tccttcctcc tcgattctag aatcaggcac
    15481 ctgtggaacc ctgcttgatc tcccgcctgg cccgccgcct ggaaaaagcc tcgcgcagct
    15541 ggtgaagccg gtcggcggtc agcggatagc gccagatgac gaacaccgag gcggcgtaga
    15601 gcagcatggg taccaccgtg atgttcagcc gcagcgcgag aagctcgtcc gggccgttct
    15661 cgcccttggg gtcgaagccg aagtaggcga cggcgggcaa cgccaggccc acggcgatgg
    15721 cctggaacag cttttggccc agcgaccaga ccgccatgta ggaacccgcc acgtgattgc
    15781 ccgttcgcag ggccgcgacg tcgatgacat ccgccttcat ggacagggag atggcggtga
    15841 agttgccgct tgcgatgccg tacagcccca ggacgatgcc caggccgacc acgtcgccgg
    15901 agccgagcat caggtatccg ggactggcaa tggcatagat ggcggcgccg agcagccagg
    15961 tctccttctt tccgatccgg taggaaatct gcagccacag cggcagggag agcagctgca
    16021 cgaagtagaa gcccagcagg atggcctggg tgtacccctc cacgtccacc acgaacgtgg
    16081 cgaacagcat gaacatggat gacgcccagg tggtgcccat ggacagcatc agaaaacccg
    16141 tgaacagcag catgaaagaa ccgttcgaga acatctgtcg gctgctcacg agcaggttgg
    16201 cctcggtggc acggtgcttc tccggctccg gcactttcca cagcgccagg gccatcagaa
    16261 ccggcagcag caccagggcc atgaagccga cgatggtcaa accttcccgg ggcagggaac
    16321 cgtagccgaa aagctccccg gagagggtgg gaatcgccag cacgctcacg ttgccgaggt
    16381 agccgaatgc ctggcgccag cccgttatgc gcgtacgttc gttgtagtcg tcggagagct
    16441 cggcgcccca ggcgaagtag ggaatgttga tcatggtcca gcccagccac agcagcatgg
    16501 accagatgaa gaggtgccag ttggtgacgg gccccatggg agcgaacagt tgccaggcgg
    16561 aaaccatcat gacgagggcg ccgccggcga gccacggctt gcgtttgccg aaccgggtct
    16621 gggtgcgatc gctgagatag ccgatcagcg ggtcggtgat cacgtcgctg atgcgcgcca
    16681 gcatgaggat caggccgatg tccgtcaggt ccaggcccag gtcccgcgag tagacgaagg
    16741 gcaggtagag cgagacgggt atcgccgcca gatagatggc gtagtcgggc agcccgaagt
    16801 agagaagggt ggaacgcgaa aggcgttttt gcgacatgag acccccgagc ctaaacgggc
    16861 gtgtcagcca ccgtcaggca gcccgccagg tcggcggcgc tcagagctgc cccagggtgt
    16921 ccgcttcctt gaggatttcc atcagcagcg tgcgggcctg ggactcgtcg gcttccgccg
    16981 tctccaggct tctggagctg attcccggta tccgggcaat catcaggttg ccggacacgg
    17041 aatatacgcc cgagtactgc ctgcctttgt actcgatagc cagctggttg gtccctgcgc
    17101 tcatagcctc cccaccgggt cacgttgttt gtcggatttg cactgaaaga taacacgatt
    17161 gggcgaacac tgaagggccc gggcgcgtct cggaaacccc gtatggtccg gcctggggcg
    17221 actgccgata gtggaaggga cgctttcctg gtgagaagag ggccaatatg gcgggcgatg
    17281 cgagagcgac cattgcgctc gatgtcagcg aatccatctg ggagcggttc ttctgcgtgt
    17341 ttcctctggt ggtggttggc acggtcgagc cggacggaac ctatgacctg gcgcccaagc
    17401 acctggccat gccgatgagc tggaagaacc acttcggctt cgtctgcacc ccgcgccacc
    17461 ggacgtatca gaacatcgag cggacgggcg aattcaccgt gacctacgcg cggccttctc
    17521 aggcggtgct ttcgagcctc gcggccaccc cgcgatgtga ggatggcagc aaacccatca
    17581 ccgccgcgtt gccgacgcgg ccagcgcaga cggttgccgg cgtctttctc gatgacggtt
    17641 acctctttct cgaatgtcgg cgctatcgcg tctatgacga cttcggcacc aacagcctaa
    17701 taaccggcga gatcgttgcc gctggcgtgg cggaggatgc tctgcgcagc gccgacgagg
    17761 acgatcagga cgtgatccgc cgggcgccgt tgctcagctt cctctatccg gggcggattg
    17821 ccgagatctc ggccagccag cagctgcccc tgcccgcggg attctccagg tagccggatg
    17881 agccgggccg atgacattcg tcgccacctc caggcggaag agccggagat gctggagctg
    17941 ctaaccgctc tggtcaccct ggagtcaccg tccgatgtcc ccgagactca ggggcccgtg
    18001 ctcgatgagc tgcagcgggt tttcgacggt ctcggctacc ggacgatccg ggtgccgggg
    18061 cgcgcctcgg gcggccatct ctatgctcgg ccgggccgcc gccgatcggg cggcaaccgg
    18121 agatggcaac tgctccttgg ccactgcgac acggtctggc cggtagggac cctggagaaa
    18181 atgcccctgg ctgcggatgg gaaccacctg aaaggtccgg gcatctacga catgaaggcg
    18241 ggcctggttg agatggtctt cgcgctcaaa gccctcgagg cgctgggcct cgagcccgaa
    18301 gtggagccgg tgattttcat caactccgat gaggagatcg gcagccggga gtccacgcgc
    18361 tacatccgcg cgctctcgca gcgggccgac cgggcgctgg tcatggagcc ttccctgggg
    18421 ccccggggcc ggctgaagac ggcgcgcaag ggcgttggcc gattcaccgt tacggttcgc
    18481 ggcaaagcgg ctcatgcggg tctggatcca acggctggcg ctagcgccat cctcgagctc
    18541 tcccacgtga ttcagaagct ctttgccctg aacgaccccg agcggggggc atccgtcaac
    18601 gtcggcgtga tcgacggtgg cgtgcgcgcc aacgtggtgg cgccggaaag cagcgccgtg
    18661 gtggatgtca gggtggccag ccaggaagat gcggagcgca tcgagcgggc gatactcagc
    18721 ctcgaagcgg aaacgccggg cgtgtctctc ggcgtggtcg gccgcatcgg ccggccaccg
    18781 ctggagcaca cgccggcgaa ccgtcagttg tggcgcgtgg ccacggatct ggcatgcgag
    18841 ctcggcattc agctggaaga agccatggcc ggcgggggct cggacggaaa caccaccagt
    18901 ctgtttaccg ccaccctgga cggtctgggc gcggtgggcg atggcgccca cgcggatcac
    18961 gagcacattc aggtagacca gctgtcccaa cgctgcgcgc tgctggccat gctcctgctt
    19021 gcccctcccc tgaactccgg aaccggttga ggatcggcgc gtgcgacacc tgctgacatc
    19081 cgttacccgc atctgcgacc ttgccgaggg cgctttcgac gtggcgccgg tcccgaaaga
    19141 cacctgggcg accggcgact acgtggtcgg ggaagtactc gatgcctccg gatatcacaa
    19201 cgtcgagctg cccaatggcc gcctgatgga agccatggaa ggcgatcggg tcgtcggcgc
    19261 tttcggggct cgagcggcca ccctcgaagc cgtgggtgac tggcagagca ttcagggcga
    19321 tgagttcgac gcgctgaccc ccgcggggct cttcggccgc ctcacctccg tgtctccttt
    19381 tctgccgagg ctaatgggca tgcgctacct ggggcacgtg cacgccaacg gcgttcgcca
    19441 gaccatggac ggggccgtga cgccggctcc tgacgcggcc ctgaccgtgc ccatcgttct
    19501 catcaccggg acctccatgt cctcggggaa gaccatgtcc ggccgcctca tcgtgaggct
    19561 gttgagccgg ttggggttca gggtcgttgg cgcaaagctc accggggcgg cccgttaccg
    19621 cgatgttctc agctacgagg atgcgggcgc agcggccgtc ttcgacttcg tggacgaagg
    19681 gttgccgtcc acggtgggtg atccggcgga ttttgccgcg cgtctggaac ggttgctcag
    19741 ccggattgct ggcgcggcgc cggacgtggt ggtggccgaa gcgggcgcct cgccgctgga
    19801 gccctacaac gggaaaacgg ccatcgacgt gctcggcgac cgggtccgtt tcaacctgct
    19861 gtgcgcatcg gacccctacg cggtggtcgg tgttgcttcg gccttcgagc gcacgcccga
    19921 tctggttgcc ggcggcgcgg cgaacaccac cgccggcatt gagctggtgt cgaagctgtc
    19981 gggcctcacg gccatgaacc tgctcggcaa ggactgccac gagcccctga cccggatgct
    20041 gcgcgacaag ctcggtcttt agccgttcag ccgacgcgcg ccagcggggt gggatgcccc
    20101 gcggcgccgg gccggcagcg ccagacgcag ccgcccaggg ctttgtcgtc cgtgttgtcg
    20161 gcgctcacga tgtacaggtc ccgatagtcc gagccgccga aggcgcagct ggtgacccag
    20221 ctggcgggca cgcggatctc gtcgacatgg ttgcccccgg ggtccagaac gaccacccgg
    20281 ccgccctgaa cgtgggcgac ccagagattt ccctcggtgt ccacgcacaa gccgtcgggg
    20341 atgccctttt cgaaggcggg cttggcgata tgtcgtctgt tggacaacgc gcccgcgtcg
    20401 tccagatcgt gcgcccagat tccccggctg gtcgagtcga cgtggtacag agtccgtccg
    20461 tcaggagaga aaccgatgcc gttggagatg cccacgccgc catagagctc cgtgacgctg
    20521 ccgtccaggt cgatgcggta ggcctccccc ggcgggtccg gctcgtcgtc caggttggcg
    20581 atgtctgcac gtatcgagcc aacgtagatg cgccccgagg cgtcggcgtg cagatcgttg
    20641 aagaagcgca cgccgtcgcg ctcgagcagc acgcgctcct gaccgtcgcg ccagaggacg
    20701 acgctgggtc cggtcatcac gaaaccgcca ttcgcgtgca tgagcaggcc gccgaccatt
    20761 cgccggtcgc gcacgacgac ctcgatgatg ccatccggat cgcgccggtt caccgtcccg
    20821 ccgtagacgt ccgaaaacca cagcgccccg tctgcgtcgg tgcgcggacc ctctagaaga
    20881 cgatagccgc ttgccaggag ttccagctcg acgttcatag gcttgtttcc cgtgctcagg
    20941 ggccgatgcg gcgcccggat gatggtcggt cgaaggatcc gtgccgaaac ttttcggcgt
    21001 cgatctccat cttgctcacc ggcccggtgg tggtgccctc gtggacgcag acgcgctcta
    21061 agatgcgcca ctcgtcgccg cgcttctcgt agacgtccag gtatcggccg taccacgtgt
    21121 cgagcacgtc ggcgccatcc tggaacagaa aggtgacgtt gtatgcctcg cctcgcgcgt
    21181 gaatgccgtc ggggtcgagc tcgatgctgt ggttgaaaat gcagtggctg gtggagcgcc
    21241 agcgcaggtg gctcaccatg cacatctctg cgaactccat cgcgttgccg acgaacacgc
    21301 cgtgatcgtc cgtggcctcc ggccagtagg ccgacttcat caacgcctcg tccaatcggt
    21361 cgacgccgcg gcagtagcgc ttcgcggcgt cccggatgca ctcgagatcc gacagttgct
    21421 caggcgtgta agccatgtgt ctctcccctg atcagctaag cggctcaatt attcaacgtc
    21481 gcgacgccgg gagccagcca catctggggg ccccgccgag gtggctgcta gaatgccgcc
    21541 ttctcccggg ggccggaggt cgagcatggt agatgactct ggaacgttcc cgacggccgg
    21601 tgtgctgggg cgggcatttc gcagcggcga gctctcgccg gttgcggtga cccgtgagct
    21661 gctcgagcgg atcgatcgac tgaacccggc tctgggcgcc gtgcatcggc tgtgccccga
    21721 acgggccatg gcggaagccg ccgcggcgga gcgggctttt tcggccggcg aggcgcggag
    21781 tcccctgcag ggtgttccct acctggtcaa agatcttttc gacgttgccg gtctgcccac
    21841 caccgcaggg acccgtctgc tggaggacaa cgtggcggag gcagattcca ctgccgtggc
    21901 caggcttgcc gctgcgggca tggtgctcct gggcaagacg gtgaccgtgc agttcgccta
    21961 cggcggggcc ggtgtgaacc acgatcacgg cacgccgcgt aacccttggc gtcaggacca
    22021 ctaccttccg gggggatcca gcagcggctc ggcggtggcg gtggcggcgg gtctcgcgcc
    22081 gttggcgctc ggcagcgata cggggggatc cgtccggatc ccggcctccc tctgcgggat
    22141 cacggggctc aagacgacgg tgggcaggat aagccgggcg ggcgtgtacc cgctcagctg
    22201 gagcctggat tccgtggggc cgctcgcccg cagcgtcgag gacgcagcac gtgtgtatca
    22261 gctgctcgcc ggggccgacc cgggcgacga aaccaccctc ggccgcccgg cgggcgacgt
    22321 gctggccggg ttggaagacg gtttagacgg gctgaagatc ggaataccgg aaaacgtgtt
    22381 ctgggaagag gtggatgccg aggtggccga agccgtgctg gccagcgccg atgtgctcag
    22441 atctctgggc gcgcgcgtcg agcgaatggc cttccccgcc gcggacggcg ccaggctgct
    22501 caatcccagg ggtctcgtca tcgcggcgga ggcctatgcc gtgaaccgcc gtttcatgga
    22561 cgaccactat gacgagctgg atccggtagt cgccacgcgc atgatccagg gcaagggcgt
    22621 cgctgccgcc gactacatcg aaaatctcct gcagtggcag gccctgcgcg tggaagcgat
    22681 gaaacagatg gcaggggtgg atgcgctcat ctgcccctcg acgatgattc cggcgacccc
    22741 gctggccgac tgcgactgcg atctcgaccg ctacgtggaa cgcaatctgg cgtatctgcg
    22801 caataccagc atcggcaacg ttctgaatct ctgtgggctc agcgtcccct gcggatttac
    22861 cgccgcgggg cttcccatcg gcgcgatgat ctgcggctct ccattcgccg aggccacggt
    22921 gctgcggatc gggcacgcct atcagcgggc tacggactgg caccagcggg t
//
LOCUS       DBNHLM010000222        11611 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1230191, whole genome shotgun sequence.
ACCESSION   DBNHLM010000222 DBNHLM010000000
VERSION     DBNHLM010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11611)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11611)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11611
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1230191"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="AC16QF_10255"
     CDS             <1..972
                     /locus_tag="AC16QF_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6448981.1"
                     /translation="VKNALWTTDPPVHSLHRKLVNKAFTRRWVEAQERAIREIASDLI
                     DAVESRGEMDFISEFAVKIPLIVIADALGVPREDIATFRHWSDDILAGNLDVLSHERR
                     REVATSWVEATRYFADTVEARRRNPRDDLISSLATAEIDGERLEVAEILPIISTLLLA
                     GNETTANLIGNGMRILLEQPEVMARLREDAGLIPPFLEEVMRFDAPVQCLYRVVTRDT
                     ELGGVTIPAGSNVMLGWGSAGHDPDFFERPEQFDLERENVNQHVGFGYGPHLCVGLML
                     ARAEARIAFETALDRLSGVRLKPGSELEYVPTYATRGLQRLDLLFDA"
     gene            complement(969..2363)
                     /locus_tag="AC16QF_10260"
     CDS             complement(969..2363)
                     /locus_tag="AC16QF_10260"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6448982.1"
                     /translation="MAKHHAHDLSRPSDPNLAPGAARCPGPSFQDIVRTEDSARSTAL
                     TTQSYDFLGDEDVPFSRYTSREMYELEIERVWSRTWQWACREEHIPSPGDYHVYDVGP
                     YSVLVIRSDPNTVRAFVNSCPHRGMQFCDSGTSGRGKQFLRCPFHGMSWHLDGSLREI
                     PCRWDFPHVEEENFGLTPVACETWGGFVFISLDPEPEPLATYLGVLPEHFSEWPLEER
                     YVALHMQKVLPGNWKMCMEGFMEAYHVMATHPEGLKTSAWATTQYDVFGRHVSRFMQP
                     RAMPSPYYGRPVSEREAFAALGHDPEDFPEGSSARQAHADSVRRSMGESLGVDLTGYT
                     NSQLLDSIEYHLFPNACLFPGIIVPFIYRFRPLTWDSCLHDILVLHPVPDSGPRPPPA
                     EPIRLGIDDSYTSVPGFSENRLALVLDQDTDNFRRQWAGMQASRKPGETLANYQEVRI
                     RHFHQTLDAYLTSA"
     gene            complement(2380..3543)
                     /locus_tag="AC16QF_10265"
     CDS             complement(2380..3543)
                     /locus_tag="AC16QF_10265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase family protein"
                     /protein_id="HIR6448983.1"
                     /translation="MPNADPFAPEIPEPVLEDLRRRLTTIRWPHVIGEDDWVYGVPQA
                     WLKDMADYWAHEWNWNSTATAMAAFDHYLADMDGIPVHFLHVPGKGPAPKPLILTHGW
                     PWTFWDYKDVIGPLSDPAAFGGDAADAFDVVVPSLPGFGFSTPLTSAGIDVAEVARLW
                     VRLMQDVCGYERFAAHGGDWGALVTAHLGHAHAQSLIGAHLALPLIPGVDRASVPREA
                     YAEDEQWMLERAAQSGPLIRSHVSVHTTDPQTLAYAMSDSPIGTAAWIWERRRNWSDC
                     DGDVERAFDRDHLCTTAALYWCTGAFTSSLRLYFEHFNKPWPLVHDRKPTVEAPTGFA
                     IFPKDVVQMPRRVAAEHTDLRRWSVMPRGGHFGAAEAPDLIVDEIRSFFRDLS"
     gene            3692..4534
                     /locus_tag="AC16QF_10270"
     CDS             3692..4534
                     /locus_tag="AC16QF_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6448984.1"
                     /translation="MAETRKVFVVLDPTTMEQASLQWGEKIALDNRAHRSQDTHLHVY
                     CCVNFKSVTAVAADTFEHVEAATLERVREWVNRIVTHTRSLGVPVDVEVEWDADWRKA
                     IVGAVRRQNPDVVIKNLTQHARLVRLVRDTSDWQLLRDCQCPVLLIKTGRLYGLKKIL
                     VAVKHTEDEEYQAANRGIMARAQGLADDFGAELHAVTCYDPDRRPDRQRFADSFGLQR
                     NQVHAAMGKPEKVLASVAEEIGADMLIIARVARPESTGLLGDTARRVIDEIATDVLVL
                     PMNG"
     gene            4599..5513
                     /locus_tag="AC16QF_10275"
     CDS             4599..5513
                     /locus_tag="AC16QF_10275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR6448985.1"
                     /translation="MESIRRALEDDLSLFAVPVFIVAVLFEALWSRGTGRRLYGVQDS
                     LVSLSMLVASAVVEILPRLLAVALMIQLHEMSPLRDVVVRQWWAWALLFVLDDFSYYW
                     FHRVNHEVRLLWAGHVNHHSSEYLNFATALRQGVGERIHKMVFWLWLPLLGFDVGMIL
                     TMIGLNLIYQFWVHTQAVDRLPAWVELIFNTPSHHRVHHASNVRYLDCNHAGVLIVWD
                     RLFGTFSEETAEDPPVYGLTRNVRSFNPAVVLFHEYAALWRDVRRAPGWRQKLGYLLR
                     APGWSHDGEDRRSRTLRRCEAAIAGTGP"
     gene            complement(5491..6681)
                     /locus_tag="AC16QF_10280"
     CDS             complement(5491..6681)
                     /locus_tag="AC16QF_10280"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6448986.1"
                     /translation="MRHALGAPRQGQEQTNMSYADGRLINDADSHLMELPDFLTAHAD
                     PGMRGRLPELNPGGQFDMSERTHQRGHPPEVVAELTALGNDLTRGPKWHDALGASSGH
                     ERRTALDLLGFQRQVVFSSFCARLIFEAEPLDIRFAAARAHNRAMAEFCSADERLIGV
                     AMVPLDDPNAAVREIAHAHELGLGAVWIAADAPGGRSPGHPEHDPIWATLSERRLPFI
                     LHVGSGVLGIPDSWMNDGRPESESARGGAEIIGSKDLTVIHHSAVRFLSVMVLDGVLE
                     RHPELRGGVIEIGAGWVPDMLRRLDHAAAIWSRSEPHLATMSRSPSRQIRDQVRFTPY
                     PFEDVAQLVRESYPELYLFSSDYPHAEGGRDPIGRFDAATATLTDSERAQFFSRNFTD
                     LYRQ"
     gene            6739..7488
                     /locus_tag="AC16QF_10285"
     CDS             6739..7488
                     /locus_tag="AC16QF_10285"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448987.1"
                     /translation="MSDAFRLDGQVAVVTGAGKGIGRGIALCLAQAGADVVVSARTLS
                     DVQGVAAEIEKLGRRALAVSADVTSAEQLETLVVAAQELGGLDIWVSNAGGLPDATPR
                     YLTRTPEDRWDAQIDLNLKAVWQGAVVAAQNMDRGVIINISSRSAQGPSFKNGPYGAS
                     KAAVNSLTQTLSLELAPNIRVNAVAPGPIPTENFNESTSFPDDKPIEQIIGVPLGRLG
                     TPEDIGNAVVFMASPAAAWITGQCLYVTGGL"
     gene            complement(7535..8359)
                     /locus_tag="AC16QF_10290"
     CDS             complement(7535..8359)
                     /locus_tag="AC16QF_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6448988.1"
                     /translation="MGSLQGKIAVVTGASRGIGKGIALALGEQGCTVYVTGRTRGDGE
                     RTIDTTARLVTEAGGEGRAIQCDHGDDEQIAALFQQVKEEAGHIDILVNNVYKIPDPP
                     AWGGGFWDHPIQVWDDQVGIGLRAHYVASWHAAPLIFAAGAGGAILNVSSPGGQSYHF
                     SSSYGAGKAGLDRLTADMAIELEPKGVAAVVLYPGSVATEFIQDHAEAQGMDLSESQT
                     PLYVGRCAVAMLAADDLMSRSGSIQWVEDLGEEFDILDEHGKRPPGYAQRPGTQAS"
     gene            8525..8944
                     /locus_tag="AC16QF_10295"
     CDS             8525..8944
                     /locus_tag="AC16QF_10295"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6448989.1"
                     /translation="MSDQYVADRIALQDVMLKYAAGVDNRDFDLYRSCFADDVEVLGF
                     GDETVNGGDAWLAYVKNALERFGPTQHMLGPQLATVDGDDAHCRTDVQALHYLKGEES
                     TTLTLWATYETDMRRIDGTWKIVRHRLVSRGTRIQSD"
     gene            8965..10224
                     /locus_tag="AC16QF_10300"
     CDS             8965..10224
                     /locus_tag="AC16QF_10300"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6448990.1"
                     /translation="MNEQEIRTLRQLMEYEVGRNSPPDGFPDLPDLPGGRYTDQRFYD
                     LEQEHIWRKSWLLAGHIDEIPEPGCFRLWETAGQPVVIVHADGGAVNAFYNTCSHRGA
                     PVVTDASGKRDRLVCRYHGWTYSHDGELLAVRDPQDFGDLDFSCRGLRKIRCERFGNL
                     IFVNFDDAAPTLLEWLGPIADEWKEFQFDNCRLAARHMWPLNCNWKIAMEANTEVYHV
                     RSIHAQTVSPMLDDRRNVNTFYPNGHGRMVAPGRRNENGELVRQRPPEMAEIETVGEI
                     GRTCTQSYGIFPNWVSPLSHVALPPLLFWPNGINKCFLETWTLAPDWGDGPKPDVWTV
                     NDGESLSPVLIEDTEFGQWIQKSVESYGFRGVPLSYQEARIYHWNQFADRMIGIENIP
                     EELRVAQVIGPEWVYPNDPRVALIAAG"
     gene            complement(10221..10994)
                     /locus_tag="AC16QF_10305"
     CDS             complement(10221..10994)
                     /locus_tag="AC16QF_10305"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6448991.1"
                     /translation="MILTSNQIDDYWAQGFVVVPDLIREVSLDRYEARFEELAIGSVA
                     PAPAMKIMRDIMFVRGAALPESALHAVNKMINFEDDAVLYAYTLEPRLVSAVRDLIGP
                     DIYSISTNIFNKPPGVDGRHPLHQDLRYFRIRPADGIVGTWTSFAATTRDSGCLAAVP
                     GSHKGDLLPHADPDWEYVNAGFLGIENQTEAQRVYLEMDPGDTLLFHPLLVHGSGRNR
                     SDHFRRAISSHYASAACESPVRDWRIGKQVRHIPAGKEG"
     gene            complement(11056..>11611)
                     /locus_tag="AC16QF_10310"
     CDS             complement(11056..>11611)
                     /locus_tag="AC16QF_10310"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017903948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HIR6448992.1"
                     /translation="LADLLYRRRIGELKIDMPLIASNHRDAYPLAAAHDVDFLHLPAS
                     PETKSQQEARLLDEVRARDIDFVVLARYMQILSAEFCAALTGRIINIHHSFLPGFKGA
                     RPYHQAHERGVKLIGATAHYVTADLDDGPIIEQDVERVDHRMSADQLAAVGRDIERVV
                     LAKAVRYQSEHRILLNGNRTVVFT"
BASE COUNT         2062 a         3560 c         3914 g         2075 t
ORIGIN      
        1 gtaaagaacg cgctgtggac gacggatccg cccgttcact cgctgcatcg gaagctggtc
       61 aacaaagcct tcacccgacg ctgggtggag gcccaggagc gcgcgatccg ggaaatcgcc
      121 agcgacctca tcgacgcggt ggagtcgcgg ggcgagatgg acttcatctc cgagttcgcc
      181 gtcaagatcc cgctcatcgt cattgctgac gcgctcggtg tgccccgcga ggacatcgcc
      241 accttcaggc actggtccga cgatattctg gccggcaatc tggatgtcct atcccatgag
      301 cgtcgtcggg aggtggcaac ttcgtgggtg gaagcgacca gatattttgc cgatacggtg
      361 gaggcccgtc ggcggaatcc gcgcgacgac ctcatcagct cgctggccac ggcggagatc
      421 gacggagagc ggctcgaggt agcggagatt ctgccgatca tctccacgct tctgcttgcc
      481 ggtaacgaga ccacggcgaa cctcatcggc aacggcatgc ggatactgct ggaacagccc
      541 gaggtcatgg cgcgacttcg cgaggacgcg ggtctgatac cgccgtttct ggaagaggtc
      601 atgcgtttcg atgcgcccgt gcaatgcctt taccgcgtgg tcacccgcga cacggagttg
      661 gggggcgtga cgataccagc ggggtcgaac gtcatgctcg gctggggctc agccggacac
      721 gatccggact tcttcgagcg tcccgagcag ttcgatctgg aacgtgagaa cgtcaaccag
      781 cacgtcggct tcggctatgg gccccatctc tgcgtcggat tgatgctggc gcgcgcagaa
      841 gccaggatcg cgttcgagac cgcgctagac cggctgtccg gcgtccgtct caaacccgga
      901 agcgagctag agtacgtgcc cacctacgcc acccggggtc ttcagcgact ggaccttctc
      961 ttcgacgcct aagcagaagt gagatacgcg tccagggtct ggtggaagtg ccggatgcgc
     1021 acctcctggt agttggcaag ggtttccccc ggctttctgg aggcctgcat gcccgcccac
     1081 tggcggcgga agttgtcggt gtcctgatcc agcacgagtg ccagtcgatt ctcgctgaat
     1141 ccgggaaccg atgtgtacga gtcgtcgatt cccaggcgaa tgggctcggc gggtggggga
     1201 cgcggaccgc tatcgggcac cggatgcaga acgaggatgt cgtgcagaca gctgtcccac
     1261 gtgagcggac gaaagcggta gatgaacggg acgatgatgc cggggaagag gcaggcgttg
     1321 gggaacaggt gatactcgat ggagtcgagc agctggctgt tggtgtatcc cgtcaggtcg
     1381 acgccgagag actcgcccat gctgcgacga accgagtccg cgtgcgcctg ccgggcgctt
     1441 gaaccctccg ggaaatcttc cgggtcgtgt ccgagcgcgg caaacgcctc tctctcgctg
     1501 acgggccgcc cgtagtaggg gctcggcatc gcccggggct gcatgaagcg gctcacgtgg
     1561 cgcccgaaga catcgtactg cgtcgtagcc caggcagatg tcttcaagcc ctccggatgc
     1621 gtagccatga catgataggc ctccatgaat ccttccatgc acatcttcca gttgccgggc
     1681 agaaccttct gcatgtgcag cgcgacgtag cgctcctcga gcggccactc gctgaagtgc
     1741 tccggcagca cgccgagata ggtcgctaac ggctccggct caggatccag gctgatgaac
     1801 acgaaaccgc cccaggtctc gcacgcgaca ggcgtcagac cgaaattttc ctcttcgacg
     1861 tgggggaagt cccagcggca gggaatctct cgcagcgaac cgtccaggtg ccaggacatg
     1921 ccgtggaacg ggcagcgcag aaactgcttg ccccgtccgg acgtacccga gtcgcagaac
     1981 tgcatgccgc ggtggggaca ggaattgaca aacgcgcgga cggtgttggg atcgctgcgg
     2041 atcacgagaa ccgagtaagg ccccacgtcg tagacgtggt aatcaccggg cgagggaatg
     2101 tgctcctctc tgcaggccca ctgccatgtg cggctccaga cacgctcgat ctccagttcg
     2161 tacatttccc tggacgtata gcgagagaag ggcacgtcct cgtcgcccag aaaatcatag
     2221 gactgcgtgg tcaaagccgt ggaccgagcg ctgtcctcgg tcctgacgat gtcctgaaag
     2281 ctcggcccgg ggcagcgcgc tgcgccgggc gccaggttcg ggtcggaggg gcggctcagg
     2341 tcgtgtgcgt ggtgcttggc catgatctcg gcgctcctgt cagctcaagt cgcggaaaaa
     2401 gctgcgaatc tcatcgacga tcagatcggg agcttctgcc gctccgaagt gtccgcctcg
     2461 cggcatgacg ctccagcggc ggagatccgt gtgctcggcg gcaacccgcc gtggcatctg
     2521 caccacgtcc ttgggaaata tggcgaagcc cgtgggcgct tcgacggtgg gcttcctgtc
     2581 gtgcaccagc ggccagggct tgttgaagtg ctcgaaatac agtcgcagcg agctggtgaa
     2641 agcgcccgtg caccaataca gcgctgcggt ggtgcacagg tgatcgcggt cgaaggcgcg
     2701 ctccacgtcg ccgtcgcagt cgctccagtt tctgcgccgc tcccagatcc acgccgccgt
     2761 tccgatgggc gagtcggaca tcgcgtacgc cagggtttgc ggatccgtcg tgtgcacgct
     2821 cacgtggctg cgaatgagag ggccggattg cgctgcgcgt tcgagcatcc attgctcgtc
     2881 ctccgcgtag gcctccctgg gtacggaggc tcggtcgacg ccgggaatca gcggcagcgc
     2941 caggtgcgcg ccgatcaggc tctgcgcgtg cgcatggccc agatgagccg tcaccagggc
     3001 gccccagtca ccgccgtggg cggcgaagcg ctcgtacccg cacacatcct gcatgagccg
     3061 aacccagagc cgggctacct cggcgacgtc gataccggca ctggtgagcg gggtggaaaa
     3121 tccgaaaccg ggcaaagagg gcaccaccac gtcgaaggcg tcagctgcat cgccgccaaa
     3181 cgcggccggg tcgctcagcg ggccgatgac atccttgtag tcccagaagg tccagggcca
     3241 tccgtgagtc aggatcagcg gctttggcgc cgggcctttg cccgggacgt gaagaaagtg
     3301 aacgggtatg ccgtccatgt cggccaggta gtggtcgaag gccgccatgg cggtagcggt
     3361 cgagttccag ttccattcgt gggcccagta gtcggccatg tccttcagcc aggcctgggg
     3421 aactccgtaa acccagtcgt cctcgccgat gacatggggc catcggatag tcgtgaggcg
     3481 ccgtcgcagg tcctcgagca cgggctcggg aatctccggc gcaaacggat cggcattggg
     3541 cattcgatcg tccggaagat gggccagtgg ggaaatctcg cagatccgct ccatctagac
     3601 aaccgtggcg tctacgggga agtgccgata tgcccgccgc gccgatgcgg tgaaacttga
     3661 ccaatcgaga tcaacgcgga gtcagcagtc gatggcagag acgagaaaag tgttcgtggt
     3721 gctcgatccc acgacgatgg agcaggcgtc gcttcaatgg ggcgagaaga tagctctgga
     3781 caaccgggct caccggtccc aggacaccca tcttcacgtt tactgttgcg tcaacttcaa
     3841 gtcggtgact gcggttgcag ccgacacctt cgagcacgtc gaagcggcca cccttgagcg
     3901 ggttcgcgaa tgggtgaaca ggatcgtcac gcacacccgg tcgcttggcg tgcccgtcga
     3961 tgtggaagtg gagtgggacg cggattggcg aaaggccatc gtgggggccg tccggcggca
     4021 gaatccggac gtggtgatca agaacctcac ccagcatgcg cgtctcgtcc gcctggtccg
     4081 cgacacgtcg gattggcagc tgctgcgcga ttgccagtgt ccggtgctcc tgatcaagac
     4141 cggtcggctc tacggcctca aaaagattct ggtggcggtc aagcacaccg aagacgagga
     4201 gtaccaggcc gccaatcggg gaatcatggc ccgggcacag ggacttgccg acgatttcgg
     4261 agcggagctg cacgcggtga cctgctacga cccggatcgg cgacccgaca ggcagcgttt
     4321 tgccgacagc tttggtctgc agcgtaacca ggtgcacgcg gccatgggca aaccggaaaa
     4381 agtgctggca tccgtggccg aggagatcgg tgcggacatg ctgatcatcg ccagggtcgc
     4441 aaggcccgag tctacggggc tgctcggcga caccgcgcgt cgggtcatcg acgagatcgc
     4501 aacggacgtt ctcgtgctcc ccatgaacgg gtgatccgga cccgcggggg cgaaccccgt
     4561 tcgatataat cggcccatcg agttcgggca gggatatcgt ggagagcatc aggcgagcac
     4621 ttgaagacga tctgagcctt ttcgcggtgc cggtgttcat cgtggcggtg ctttttgaag
     4681 cactgtggtc ccgtggcacc gggcgccgtc tttacggggt gcaggactct ctggtatccc
     4741 tgagcatgct ggtggcgtcc gccgtggtgg agattctgcc ccgtcttctt gccgtggcgc
     4801 tgatgattca gctgcacgag atgagtccgc tgcgggacgt cgtggtgcgc cagtggtggg
     4861 cgtgggcgct gctgttcgtt ctggatgact tttcttatta ctggtttcac cgggtcaatc
     4921 acgaggtgcg tctgctctgg gcaggccacg tcaatcatca ctcctctgag tatctgaact
     4981 tcgccaccgc cctccggcag ggcgtcgggg agcgcattca caagatggtc ttctggcttt
     5041 ggctgccgct gctcggattc gacgtcggca tgatcctgac catgatcggg ctcaacctga
     5101 tctatcagtt ctgggtgcac acgcaagccg tggatcggct tcccgcctgg gtggagctca
     5161 tcttcaacac gccttcgcat caccgggttc atcacgccag caacgtccgc tacctggact
     5221 gcaaccatgc gggtgtgctg atcgtctggg atcgactgtt cgggacgttc agcgaggaga
     5281 cggcggagga tccgcccgtg tacgggctga cgcggaacgt tcgctctttc aatcctgccg
     5341 tggtcctgtt ccacgagtac gcggctttgt ggcgtgacgt ccggcgcgcg cctggatggc
     5401 gccagaagct cggctatctt ctccgggctc ccggctggag ccacgacggc gaggaccggc
     5461 gttcccggac gctgcggcgt tgcgaggccg ctattgccgg tacaggtccg tgaagttgcg
     5521 cgagaagaac tgggctcgct cggaatcggt cagcgtggcg gtggccgcgt cgaagcggcc
     5581 gatggggtcg cgtccgccct cggcatgagg gtaatccgac gagaagagat acagctcggg
     5641 ataggactca cggacgagtt gggcgacgtc ctcgaaagga tagggcgtaa accgcacctg
     5701 atcgcgaatc tgccgggacg gcgatcggga catggtggcc aggtgcggct cggatcggga
     5761 ccagatggcg gcggcgtggt ccagccttct cagcatgtcg ggcacccagc ctgcgcctat
     5821 ctcgatcacg ccaccgcgca attccgggtg ccgttccagg acgccgtcga gaaccatgac
     5881 ggacagaaaa cggacggcgc tgtgatggat cacggtgagg tcctttgacc cgatgatctc
     5941 ggcgccgccg cgggccgact cgctttccgg ccggccatcg ttcatccacg aatcggggat
     6001 gccgagcacg ccgctgccca cgtgcagtat gaaaggcaga cggcgctcgc tgagggtggc
     6061 ccagatggga tcgtgctcgg ggtgcccggg agaacgtcct ccgggcgcat cggcggcgat
     6121 ccacacggcg cccaggccga gctcgtgagc gtgggcaatc tcgcgcacgg cggcgttggg
     6181 gtcgtcaagc ggcaccatgg ccacgccgat cagccgctcg tctgcactgc agaattcagc
     6241 catcgctcgg ttgtgcgccc gggccgcggc gaagcgtatg tccagaggtt ccgcctcgaa
     6301 gatcagccgc gcgcagaacg aagagaacac gacctgacgc tgaaaaccga gcaggtccag
     6361 ggcggtacga cgttcgtgcc cgctggaagc gccaagcgcg tcatgccatt tcggcccgcg
     6421 cgtcaggtcg ttgccgagcg ccgtaagctc ggcgacgacc tcgggtgggt gaccgcgctg
     6481 gtgggttcgc tcgctcatgt cgaactggcc tcccggattg agctcgggaa gacggccacg
     6541 catgcccggg tccgcatggg cagtgaggaa gtccggcagc tccatcaggt ggctgtcggc
     6601 atcgttgatc agacgcccat cggcgtagct catgttggtc tgctcctgac cctggcgcgg
     6661 cgcgcctagt gcgtgccgca tgaatcatgc cgggaagggt ttagtatagt acgaagcttt
     6721 caaagagagg gtgtgacgat gtccgacgcg tttcgactgg acggccaggt ggcggtggtt
     6781 acgggggctg gtaagggaat cggccgggga atagcgcttt gccttgcaca agcaggggca
     6841 gacgtggtgg tttcggcccg cacgctgagc gacgttcagg gggtggctgc agagatcgaa
     6901 aagctcgggc gtcgggcgct ggccgtatcg gcggacgtca ccagcgcgga gcagctcgag
     6961 acgctggtgg tggcggcaca ggagctgggc gggctcgaca tctgggtcag caatgcgggc
     7021 ggtctgccag acgcgactcc gcgatacctg actcggacgc cggaggaccg ctgggatgcg
     7081 cagatcgacc tcaatctcaa agccgtgtgg cagggtgccg tcgtcgcggc gcagaacatg
     7141 gatcgcggtg tcatcatcaa catatcctca cgatccgctc agggcccttc tttcaagaac
     7201 gggccttacg gtgcctccaa ggcggccgtg aacagcctta cccagacgct ttcgctggag
     7261 ctggcgccga acatccgcgt gaacgccgta gcgcccggac cgatcccgac ggagaacttc
     7321 aacgaatcca cgagcttccc ggacgacaaa cccatcgagc agatcatcgg ggtgcccctg
     7381 ggacggctcg gcacgcccga ggacatcggc aatgccgtcg tgttcatggc ctcgccggcg
     7441 gcggcctgga taacggggca gtgcctctac gtgaccggcg ggctttaggc gatcgcccgt
     7501 cttccccgga gcaaggagag gaccgggccc cgccttacga ggcctgggtg cccggccgct
     7561 gagcgtagcc gggcgggcgc ttgccgtgct cgtcgaggat gtcgaactcc tcgccgaggt
     7621 cttccaccca ctggatcgac ccgctgcgtg acatgaggtc gtcggcggcc agcatcgcaa
     7681 cggcgcaccg cccgacgtag aggggcgtct gggattcgga aagatccatc ccctgggctt
     7741 ccgcgtggtc ctggatgaac tccgtggcga cggagcccgg gtacagcacc accgcggcca
     7801 cccctttggg ctcgagctcg atggccatgt cggcggtgag tcggtccagg ccggctttgc
     7861 ccgcgccgta ggaagaagag aagtgatagc tctgcccgcc gggcgacgac acgttgagaa
     7921 tggcgccgcc agcacccgcc gcgaaaatca gaggggcggc atgccagctg gccacgtagt
     7981 gggcgcgcag gccaatgccc acctgatcgt cccagacctg tatcgggtga tcccagaaac
     8041 cgccgcccca ggcgggcgga tcggggatct tgtagacgtt gttgacgaga atgtcgatgt
     8101 gcccggcttc ttccttgacc tgctggaaca gggcggcgat ctgctcgtcg tccccatggt
     8161 cgcactgaat ggctcggccc tcgccccctg cctcggtcac cagccgggcg gtggtgtcga
     8221 tggtccgctc accgtcgccg cgtgtgcggc cggtcacgta gacggtgcag ccctgctcgc
     8281 ccagagcaag ggcgataccc ttgccgatgc cccggctggc cccggtgacc acggcgattt
     8341 ttccttgtaa cgaacccata tctgtttccc aatgcagtga gcggggcgcg caggatacca
     8401 gaagcctgcc cacccaggca ccggggcgcg gcgagattgc cagtgctcgc cggcggcccg
     8461 cccggctggt gcgctcttcc tttatgggct agatttgaag gacattggct cgagggagag
     8521 agggatgagt gatcaatacg tggctgaccg catcgccctg caggacgtca tgctcaaata
     8581 cgccgccggg gtggataacc gcgatttcga cctttaccgg tcgtgcttcg ccgacgatgt
     8641 ggaagtgctg ggattcggcg acgagaccgt caacggcggc gatgcgtggc tggcttacgt
     8701 caagaacgcc ctggaacgtt tcgggcccac tcagcacatg ctgggcccgc agctggccac
     8761 cgtggacggc gacgatgcgc actgccggac cgacgtgcag gcgctgcatt atctgaaggg
     8821 agaggagagc accacgctga ctctctgggc gacctacgaa acggacatgc gccgaatcga
     8881 tggcacgtgg aagatcgtcc ggcacaggct ggtgtcgcgg gggacacgga tccaatcaga
     8941 ctgaacggtt gaggaatcga tcctatgaac gagcaggaaa tccgcacgct caggcagctg
     9001 atggagtacg aggtcggccg caattcgccg cccgacgggt tcccggacct gccggacctt
     9061 ccaggagggc gctacacgga tcagcgcttc tacgatctgg agcaggagca catctggcgc
     9121 aagtcctggc tgctggccgg ccacatcgac gagatccccg agcccgggtg cttccgcctg
     9181 tgggagaccg ccgggcagcc ggtggtcatc gttcatgccg atggcggcgc ggtcaacgcg
     9241 ttctacaaca cctgcagcca ccggggcgcg ccggtggtga ccgatgcgtc gggaaagcgc
     9301 gacaggctcg tctgccgtta ccacggctgg acctacagtc acgacggcga gcttctggcc
     9361 gtccgggatc ctcaggactt cggcgatctg gatttttcct gtcgcggcct gcggaaaatc
     9421 cgctgcgagc gcttcggcaa tctcatcttc gttaatttcg acgacgctgc ccctacgttg
     9481 ctcgagtggc tcggccccat cgccgacgag tggaaggagt tccagttcga caactgccgc
     9541 ctggctgctc gccacatgtg gccgctcaac tgcaactgga agatcgcgat ggaagcgaac
     9601 accgaggtct accacgttcg cagtatccat gcgcagacgg tctcgccgat gctcgacgat
     9661 cgtcgcaacg tcaacacgtt ctaccccaac gggcacggcc ggatggtcgc gcccggtcgc
     9721 aggaacgaaa acggcgagct tgtgaggcaa cgcccgccgg agatggccga gatcgaaacg
     9781 gtgggggaga tcggcaggac gtgcacccag tcatacggca tcttccccaa ctgggtttcg
     9841 ccgctcagcc acgtggccct gccgccgctg ctgttctggc cgaacggtat caacaagtgc
     9901 tttctggaga cgtggacgct ggcgccggac tggggtgacg ggccgaagcc cgatgtctgg
     9961 accgtcaacg acggcgagag cttgagccct gtgctcatcg aggacaccga gttcgggcaa
    10021 tggattcaga aatcggtgga gtcgtacggg ttccgcgggg tgccgctgag ctatcaggag
    10081 gcgcgcatct atcactggaa ccagttcgcg gaccggatga tcggcatcga gaacatcccg
    10141 gaggagttgc gggtggccca ggtcatcggc cccgaatggg tctatccgaa cgacccgcgg
    10201 gtggcgctga tcgcggcagg ttaaccctct tttccggccg ggatgtggcg cacctgcttg
    10261 ccgatgcgcc agtctctgac gggcgattcg caggccgcgg aagcgtagtg tgaagagatg
    10321 gcccgtcgga agtggtccga ccggttccgg ccggaaccgt gcaccagcag cggatggaac
    10381 agcagggtgt cgcccggatc catctccagg tatacgcgct gggcttcggt ctggttttcg
    10441 atgcccagga atcccgcgtt gacgtattcc cagtcagggt ccgcatgcgg gagcaggtcc
    10501 cccttgtggc ttccgggcac cgcggcgagg catccggaat cccgggtcgt cgcggcaaac
    10561 gacgtccatg tgcccacgat gccgtccgcc ggccttatcc ggaagtagcg cagatcctga
    10621 tgcagcgggt ggcggccgtc cacgccgggc ggcttgttga agatgttcgt cgatatgctg
    10681 taaatgtcgg gcccgatcag atcgcgcacc gcactcacca gccggggctc gagggtgtaa
    10741 gcgtagagga cggcatcgtc ctcgaagttg atcatcttgt tcaccgcgtg gagagccgat
    10801 tcgggcagcg ccgctccacg gacgaacatg atgtcgcgca tgatcttcat ggccggcgcg
    10861 ggcgcgaccg agccgattgc cagttcctcg aagcgcgcct cgtaacgatc cagcgagacc
    10921 tcgcggatga gatcgggcac caccacgaag ccctgggccc agtagtcgtc gatctgattg
    10981 ctcgtcagta tcattagccc gtttcgactg caggccgcgc ttggcaccca tcggggggct
    11041 ggcgcccgcc aagcctcagg tgaaaaccac cgttctgttg ccgttcagca ggatccggtg
    11101 ttccgactgg tatcttaccg cctttgccag cacgacgcgc tcgatgtcgc ggcccaccgc
    11161 tgcgagctgg tcggccgaca tgcggtggtc gacgcgctcc acgtcctgtt cgatgatggg
    11221 cccgtcgtcc aggtccgctg tgacgtaatg cgcggtagcg ccgatcagct tgacgccccg
    11281 ctcgtgagcc tggtgataag gacgggcgcc cttgaagccg ggcagaaacg agtgatggat
    11341 gttgatgatc cggccggtca gggcggcgca gaactcggcg ctgagtatct gcatgtagcg
    11401 ggccaggacg acgaagtcga tgtcccgggc tcgcacttcg tccaggagac gcgcctcctg
    11461 ctgcgatttg gtctcggggc tggccggaag gtgcagaaag tcgacgtcat gggcggccgc
    11521 cagcgggtag gcgtcgcgat gattcgaggc gatcaagggc atatcgatct tcagctcccc
    11581 gattcgcctg cggtacagga gatcggccag g
//
LOCUS       DBNHLM010000223        11914 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_331039, whole genome shotgun sequence.
ACCESSION   DBNHLM010000223 DBNHLM010000000
VERSION     DBNHLM010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11914)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11914)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11914
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_331039"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="AC16QF_10315"
     CDS             <1..387
                     /locus_tag="AC16QF_10315"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6448993.1"
                     /translation="EGFYDGLIFHRVIANFVVQTGGYDADMGYREPPRTVVNESANGL
                     KNQRGYLAMARLNDPDSADAQFYINVRDNTHLDGKPGSPGYTVFGKVISGWEIVVEIE
                     LSDTVVRAGMVGVPEVPIVIRRATRG"
     gene            463..969
                     /locus_tag="AC16QF_10320"
     CDS             463..969
                     /locus_tag="AC16QF_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR6448994.1"
                     /translation="MRTIVVAFATAIFFAAVIVELTDRLWPGSQVALLIVATIGLFLS
                     NLLALRLSGSPAAAAPAKRRSEAPGKQGQQKRPARQEKRPEPGTARSAAGPREPGKVK
                     WFNRNKGFGFIIRESGEEIFVHQRSIRPVGEGDNRRRPSLRDGQAVTFQVAERDKGIQ
                     AEDVVAED"
     gene            977..2686
                     /gene="argS"
                     /locus_tag="AC16QF_10325"
     CDS             977..2686
                     /gene="argS"
                     /locus_tag="AC16QF_10325"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR6448995.1"
                     /translation="MNVRALIGERIEAALAETGNPGAPALVQPASRPEFGDYQANGVM
                     GAAKRSGTKPRELADAVLKAAALDDVAAGVEVAGPGFLNITLADDFLSAQLLPEDRMS
                     AVEHPQTVVVDYSSPNLAKAMHVGHLRSTIIGDAVARVLEALGHTVIRQNHVGDWGTQ
                     FGMLLTHMTETGADSNTLSDLEDFYREAKARFDADPDFAERSRQTVVRLQSGDPDTRA
                     HWSRFIDISLSHCQAIYDRLGVKLTAADVAAESAYNDALPEVIEALRASGLLEESDGA
                     LCVFLDEFTGKDDQPLPVIVRKSDGGYLYATTDLAAIAHRSRTLHADRAMYFTDARQA
                     LHFRQVFAVARTAGLAAPEMRLDHLPFGNMLGKDGTPFKTREGGVVRLEELLDEAEVR
                     AYALVGEKSPDLPETERRQIARAVGIGAIKFADLSKNRTSDYVFDWDQMLSFEGNTAP
                     YLQYAYSRIRSVFRRGDVQPDALDTPARVEMSTPEERGLAVMLVRFQEVLEQVATEAC
                     PHFLCAYLYELASAFTRFYEACPILTADEPLRASRLFLADRTATTLEQGLGLLGIETM
                     ERM"
     gene            complement(2691..3896)
                     /locus_tag="AC16QF_10330"
     CDS             complement(2691..3896)
                     /locus_tag="AC16QF_10330"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DesA family fatty acid desaturase"
                     /protein_id="HIR6448996.1"
                     /translation="MSVILEWLSGGILNLSVLGMVIFTLVVTHVTIVAVTVYLHRHAA
                     HRSVEVHPALGHFFRFWLWLTTGMITREWASIHRKHHAVCETEEDPHSPKIKGLGAIL
                     WRGAEAYREASKQREIMDRYGAGCPDDWVERNVYQRFNFLGIVLMLIIDVALLGVAGI
                     TVWAVQMIWIPFFAAGVINGIGHYWGYRNFECPDAATNISPWGILIGGEELHNNHHTY
                     PNSAKLSQKPWEFDIGWFWIRLFETFGLARPLSTGPVVERVSGKTAIDVDTAWAVLND
                     RFRVMARYAEEVVAPLVEQEYHRADHATRALFKKAKKTLCREDSLVDAAGKTRIAELI
                     GHSPEIREIYEFRLRLQDVWAKRAAGADALLNELKQWCADAEATGIEVLRDFVEELKS
                     YSIPRLARA"
     gene            4113..4925
                     /locus_tag="AC16QF_10335"
     CDS             4113..4925
                     /locus_tag="AC16QF_10335"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR6448997.1"
                     /translation="MFTTLISADDLTSRLESGGDGLVVLDCRARLGEPDYGRRAYAAG
                     HLPGAVHVDLDADLAAPPGAGGRHPLPDRAELAAKLRERGLNDADQVVVYDDAGGAFA
                     SRAWWCLRWLGHRAVALLDGGLEQWPGPLSSQLPDVTPGDFSLRPPLTRTIDADRLAA
                     SLATLTLVDARTRARFEGHEELIDPVAGHIPGAVCLPFGENLDGDGRFLDARTLARRF
                     EGLGADIVCYCGSGVTAAHNVLAMTIAGLDEPILYPGSWSEWIVDPTRPRAP"
     gene            4955..6712
                     /gene="mnmC2"
                     /locus_tag="AC16QF_10340"
     CDS             4955..6712
                     /gene="mnmC2"
                     /locus_tag="AC16QF_10340"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmC2"
                     /protein_id="HIR6448998.1"
                     /translation="MTWRDRQPYSAAFGDIYHDGTQAEEVNRVFVAPSRLVEKAREQR
                     SLVVGELGFGTGLNFVVAAAACLQASARLHFVSFEAAPISPPEFAALSASRAGQHPLY
                     GELGRQYPPLVRGWHRRVLSNGQITLSLYWGDAEAGLDDIDGRQLRPFDVWFLDGFAP
                     DRNPAMWTPTLLEKVGALAGDASTVATFTAAGRVRRALEAAGYNMRRVDQRPHKRESL
                     AGERRHGGRPGFAPPAHVRVAGAGLAGASVARQLAEIGIDVTVHEAGSAPGSGASGIP
                     ATVLHPRLLGDGSVQAALRCHAYLFSHPYLARWLSDPTRGVLQLPGERQGAQGIERIA
                     DRYAGTGSWLEVLPPHRAAAVADWPVASPGLWFPDGRVVDTPGLTRALLDHPRIELVT
                     GSAITTLDAPGTEATVLACGADCARFEEARYLELSPVDGQIDILASDRLPRLPLVGNG
                     YVVPGAGGLVVGTTYEYRPWPAGSATAANLRQLPEDVGRAARWLGRARGTRAVTSDRL
                     PVAGRLFDLNAHPLTNAYATTGHGSMGTVFAPFAGAVVASLISGDFSPLERDLEQALS
                     SARFRTRQARRGYRHGAQP"
     gene            complement(6718..7896)
                     /locus_tag="AC16QF_10345"
     CDS             complement(6718..7896)
                     /locus_tag="AC16QF_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR6448999.1"
                     /translation="MYKIRTLNNIAVRGLERFSREHYEVGSEIADPHAILLRSHQLAP
                     TDLNESLRAVARAGAGVNNVPVADCTEKGIVVFNTPGANANSVKELVLAALLISARDV
                     VGGIEFVSSLGDIEDEDELDRLVEAEKKRFKGRELQGKSLGVVGLGAIGSLVARAALD
                     LGMEVLGYDPAISVDAAWRLPSQVRRMENLPSLFARADYVTLHVPLLPETTGMINDES
                     LRLFRPGSVLLNFARQPIVDSGALRAALDDGRVALYVADFPVPGLTDHPNVRLTPHLG
                     ASTEEAEENCAVMAAEQLIEFLETGNIRNSVNFPSVNLEPSKGYRLAITNRNVPGMLG
                     KMTSILAESNINVIDMLNKSRDEVAYNLIDVAEEPAPELIDAICAIESVMNVREFKID
                     "
     gene            complement(7986..9053)
                     /locus_tag="AC16QF_10350"
     CDS             complement(7986..9053)
                     /locus_tag="AC16QF_10350"
                     /inference="COORDINATES: protein motif:HMM:NF009102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR6449000.1"
                     /translation="MSHVVFTGGGTAGHVVPTFPVIAALAGRGVRISFVGSNSGLEAE
                     LLEGVDADFYGIAAGKLRRYLSVENLRDVFRVLLGVWQAFRLLGRLRPDVVFSKGGFV
                     SFPVVFAAWLRRVPVVAHESDLTPGLANRLALPFLRTVCTSFSSTAFPGFKGRVVHTG
                     TPLREALLAGDRARGRQRLGAGKDERVLLITGGSLGADVINQVVREVLDELLASWLVV
                     HVCGPGKVTGTAQPRYLQFEYVTEGWGDILTAADLVVSRAGANTLYELLALGKPNLLI
                     PLSRRASRGDQLENAEFALAQGYSRVLLEEDLDGPALLREIAALDAQREAAAARLHGF
                     APPDAVELICSELTPFLPAGS"
     gene            complement(9050..9748)
                     /locus_tag="AC16QF_10355"
     CDS             complement(9050..9748)
                     /locus_tag="AC16QF_10355"
                     /inference="COORDINATES: protein motif:HMM:NF047237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdaA/DnaA family protein"
                     /protein_id="HIR6449001.1"
                     /translation="MTGPRQQLSLPFPINQRFVLGNFEIGENAEVVQRLEAEPDRSVF
                     SGLWLWGGGAVGVSHLLHGVCHWHAARGARVAYLPLGAVERDPAVLEGLDFYDLLALD
                     DVEAWAGDLQLESPLMAMYERLFARRARLIVGAAGPAARCGFRLADLASRLSSLTAYE
                     VRPLNDEGKVRLLRRLAKERGLELSDAVLGYWLARSHRSVRRLLDDLEVIDAEAMRAQ
                     RVVTIPLLKDALGL"
     gene            complement(9762..10337)
                     /locus_tag="AC16QF_10360"
     CDS             complement(9762..10337)
                     /locus_tag="AC16QF_10360"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR6449002.1"
                     /translation="MFTTAQIPNLITVARIAMVAPVGLLLWYGSVREALILMAVASAS
                     DALDGWLARHFGWMSRFGAAMDPVADKLLVGTLFVIFTLKGIIPIWVALIVLGRDVII
                     LGGALAYRLLFETIEFAPTYLSKANTAVQIVLLLLLLIAQCGFDWISGAAALLADPYG
                     FWVLAALGLTSGLDYVITWSLRAVRRAKAAL"
     gene            complement(10338..11411)
                     /locus_tag="AC16QF_10365"
     CDS             complement(10338..11411)
                     /locus_tag="AC16QF_10365"
                     /inference="COORDINATES: protein motif:HMM:NF021367.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HIR6449003.1"
                     /translation="MPTVVSARSHLLKVTLAFLLTGGAVADVVPWLYEVEVPVENQSR
                     EARLDASGEALLQVLTRVTGLTSVPRVEAVREALSAPGRYYNQFRYLEVEGVGADGRP
                     IEELKLIVQFERGSLLQLVKDAELPVWRSNRPRVAAWIVLDRGGARSILSPEDAAGLA
                     SELSARARDRGVLVSLPLMDLQDSMLVDPAAVWGRVSTVLEPASARYGADVVLVGRLQ
                     QIGAEQWTGAWDFWLDGDLHPFSMSAENAVRLVEAGVDFLADELAQRHAVLGRFARPV
                     LVGVSGVGSARDYGELLSYLAELEFVDDIAVAGVKGDRLGLVLTTRADIEQLLANFRF
                     DRRLAELPGAVPGSADVELLWRR"
     gene            11516..>11914
                     /locus_tag="AC16QF_10370"
     CDS             11516..>11914
                     /locus_tag="AC16QF_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HIR6449004.1"
                     /translation="MTKGLTYRDAGVDIDAGNELVRRISAACKATQRPEQLTGLGGFA
                     ALAAMPERYRQPVLVTGTDGVGTKLKLAIDHDRHDQVGQDLVAMCVNDVLVTGAEPFL
                     FLDYYATGRLDVDVAERVIKGIAHGCELSGC"
BASE COUNT         2069 a         4112 c         3873 g         1860 t
ORIGIN      
        1 gagggtttct acgacgggct gatcttccac cgggtgatcg cgaacttcgt cgtccagacc
       61 ggcggctacg acgcagacat gggctaccgg gaaccgccgc gaacggtggt caacgagtcc
      121 gccaacggtc tgaagaatca acgcggctac ctcgccatgg cacgcttgaa cgaccctgat
      181 tccgccgatg ctcagttcta catcaacgta cgggacaata cccacctcga tgggaagcca
      241 ggttcgccgg gctacacggt gttcggcaag gtcatctccg gctgggagat cgtggtagag
      301 atcgagctct ccgatacggt ggttcgcgcc ggcatggtcg gcgtgcccga ggtgcccatc
      361 gtcatccgca gggcgacgcg aggctaaccc cagcaagcaa ttgcacatcc gcttgggtat
      421 gattcaccgt tttttcgctt cagcgctgcc tggacaccgc ttatgagaac catcgtcgtc
      481 gcgttcgcca ccgccatctt ctttgccgcc gtgatcgtcg agctgaccga tcgcctgtgg
      541 cctggcagcc aggtggcgct gctcatcgtt gccaccatcg gcctgttcct gagtaacctg
      601 ctggcgctgc ggctgtccgg cagcccggca gcggcggccc ccgcaaagcg ccgctccgag
      661 gcgcccggca agcagggcca gcagaaacga ccggcccgcc aggaaaaacg tccggagccc
      721 ggaacggccc gcagcgcggc aggaccgcgc gaaccgggca aggtgaagtg gttcaaccga
      781 aacaagggct tcggcttcat catccgcgaa tccggcgagg agatcttcgt tcaccagcga
      841 tcaatccgac ctgtgggcga gggggacaac cgacgccggc cgagcctgcg cgacggtcag
      901 gcggtgacct tccaggttgc cgagcgcgac aagggtattc aggcagaaga cgtcgtcgcc
      961 gaggactgat ccgctcatga acgtccgcgc actgatcggc gaacggatcg aggcggcgct
     1021 ggccgaaacc ggaaacccgg gggccccggc tctggttcaa ccggccagcc gtcccgagtt
     1081 cggcgactac caggccaacg gggtgatggg agcggcaaag cgctccggga ccaagccgag
     1141 ggaactcgcc gacgccgtcc tgaaggccgc agcgctggac gacgttgcgg cgggcgtgga
     1201 ggtggcgggc cccggcttcc tgaacatcac gctggcagac gacttcctgt cggcacagct
     1261 gctgccggaa gatcgcatgt cggccgtcga gcacccgcaa accgtggtcg tggactactc
     1321 cagccccaat ctcgccaagg ccatgcacgt cggccatctg cgcagcacga tcataggcga
     1381 tgccgtcgcg cgcgttctcg aggccctcgg gcacacggtc attcgccaga accacgtggg
     1441 cgactggggt acccagttcg gcatgctgct cacacacatg accgaaaccg gcgcggactc
     1501 gaacaccctt tcggacctgg aggacttcta ccgggaagcc aaagcacgct tcgatgccga
     1561 ccccgacttc gccgagcgca gccgccagac cgtggttcgt ctgcagtcgg gagacccgga
     1621 cacccgcgcc cactggtccc ggttcatcga catttcgctg tcacactgcc aggcgatcta
     1681 cgaccgcctg ggcgtgaagc tcacggcggc cgacgtggca gcggagagcg cctacaacga
     1741 cgcgctgccc gaggtgatcg aagcgctgcg cgcaagcggc cttctcgagg agtcggacgg
     1801 ggcgctttgc gtgtttctcg acgagttcac gggcaaggac gatcagccgc ttcccgtgat
     1861 cgtgcgaaag tcggacggcg gctatctgta cgcgaccacc gacctcgcag ccatcgcgca
     1921 ccgcagccgg acactgcacg cggatagggc catgtatttc accgacgcac ggcaggcgtt
     1981 gcactttcgc caggtcttcg cggttgcccg caccgcgggg ctcgccgccc cggagatgcg
     2041 cctggaccac ctgcccttcg gaaacatgct cggcaaggac ggcacgccgt tcaaaaccag
     2101 agaaggcggc gtggtacgac tcgaggagct gctcgacgag gcggaggtgc gggcttacgc
     2161 cctcgtgggc gagaagagtc ccgatcttcc ggaaaccgag cggcgtcaga tcgcccgggc
     2221 ggtgggcatc ggcgccatca agttcgccga cctgtccaag aaccggacga gcgattacgt
     2281 tttcgactgg gatcagatgc tttctttcga ggggaacacc gccccctacc ttcagtacgc
     2341 ctactcacga attcgcagcg tgttccggcg cggcgacgtt cagcccgatg ctctggacac
     2401 gccggcccgc gtagagatga gcacgcccga ggaacgtggg ctcgccgtca tgctggtgag
     2461 attccaggaa gtgctcgagc aggtagcgac cgaagcttgc ccgcatttcc tgtgcgcgta
     2521 tctctacgag ctggcgagcg cgttcacccg cttctacgag gcctgcccca tcctgacagc
     2581 cgacgagccg ctgcgcgcgt cgcgtctgtt cctggcggac cggacggcca cgaccctcga
     2641 gcagggactc ggcctgctcg ggatcgagac tatggagaga atgtgaggcg tcaggcccgg
     2701 gccaggcgcg gaatcgagta ggacttcaac tcttcgacga agtcccgcag cacctcaatg
     2761 ccggtcgctt cggcatccgc gcaccactgc ttgagctcgt tgaggagcgc gtcggcaccg
     2821 gccgcccgct tcgcccagac atcctgcaac cgcagcctga actcgtagat ctccctgatc
     2881 tcggggctgt gcccgatgag ctcggcgatg cgcgtcttgc cggcggcgtc cacgagagag
     2941 tcctcgcggc aaagcgtctt cttggccttc ttgaacagcg cgcgggtcgc gtgatccgcg
     3001 cggtggtatt cctgctccac cagcggcgcg acgacttcct cggcatagcg agccatgacg
     3061 cggaagcgat cgttgagcac cgcccaggcc gtgtcgacat cgatggcggt ctttccgctg
     3121 acccgctcta ccacagggcc cgtgctcagc ggacgggcaa ggccgaacgt ctcgaagagc
     3181 cggatccaga accagccgat gtcgaattcc catggcttct gagaaagctt ggccgaattc
     3241 ggataggtgt gatggttgtt gtgaagctcc tcgccgccga tgaggatgcc ccagggcgag
     3301 atgttcgtcg ccgcgtcggg gcactcgaaa ttccgatagc cccagtagtg tccgatcccg
     3361 ttgatcaccc cggccgcgaa gaaggggatc cagatcatct ggaccgccca cacggtgatg
     3421 ccggcaacgc cgagcaaggc cacgtcgatg atcagcatca gcacgatgcc gagaaagttg
     3481 aatcgctggt agacgttgcg ctcgacccag tcatccgggc accccgcgcc gtagcgatcc
     3541 atgatttcgc gctgcttgga ggcttcccga taggcctccg cgccgcgcca gagaatggcg
     3601 ccaagccctt tgatcttggg gctgtgcgga tcttcctcgg tctcgcagac cgcatggtgc
     3661 ttgcggtgaa tggacgccca ctcgcgggtg atcatccccg tggtgagcca cagccagaat
     3721 cggaagaaat gccccagagc cgggtgcacc tcgacggagc ggtgcgccgc gtgccggtgc
     3781 aggtagacgg tgaccgcgac gatggtcacg tgagtgacga cgagggtgaa gatcaccatg
     3841 cccaggacgg acaggttcaa aatgccacca ctgagccatt ccagaatcac gctcacgcct
     3901 acagacctcc ttgtgcacag ctcccccggg agcagatcgc gcggcattgt gacataggct
     3961 gctcgaattg ccaccttgcc tatcaacgcc taccagtata agaccgccgc aacgggcaaa
     4021 ggttacatcc gctgcgacat gagacctcca tcgtgctaaa aggcagcctc tgagagccag
     4081 ttcacgatat ccacccggca acgccctggg ccatgttcac cactctgatt tcggctgacg
     4141 atctgacctc ccgcctggag agcgggggcg acggtctcgt tgtgctggac tgccgcgcac
     4201 ggctgggcga accggactac ggccgccgcg cgtacgccgc agggcatctc cccggagccg
     4261 tgcacgtgga cctggatgcg gacctggccg ccccgccagg ggccggcggc cgccaccccc
     4321 tgccggaccg ggcggagctg gccgcgaagc tcagggagcg cggactgaac gatgccgatc
     4381 aggtggtggt ttacgacgat gccggcggcg cgttcgccag ccgggcctgg tggtgcctgc
     4441 gctggttggg ccaccgcgcc gtcgcccttc tggacggcgg cctcgagcag tggcccggac
     4501 cgctctcctc ccagcttccc gacgtcacgc ccggagattt cagcctgcgg ccgcccctga
     4561 cccggacgat cgatgcggac cggctcgcgg catcactggc cacccttacc ctggtagatg
     4621 ccagaacgcg cgcgcgtttc gagggccacg aagagctcat cgatccggtc gccggccaca
     4681 tccccggcgc cgtctgcctg ccctttggtg agaacctgga cggcgacggc agatttctcg
     4741 acgcccggac gctggcccgg cgcttcgaag ggctgggggc agacatagtc tgctactgcg
     4801 gctccggcgt cacggccgcc cacaacgtgc tggccatgac catcgcgggg ctcgatgagc
     4861 cgatcctcta tcccggctcc tggagcgagt ggatcgtcga ccccacccgg ccgcgggcac
     4921 cttgacgcgt cgggagaccc tagctcctgg cgttgtgacg tggcgcgacc gccagcccta
     4981 ctccgccgcg ttcggcgaca tctaccatga cggcacccag gccgaggagg tcaaccgggt
     5041 cttcgtcgcg ccgtcccgac tcgtcgagaa ggcgagggag cagcggtccc tggtcgtggg
     5101 cgagctcggc ttcggcaccg gtttgaactt cgtcgtggcg gcggccgcgt gcctccaggc
     5161 gagcgcccgt ctgcatttcg tcagcttcga agcagccccc atctcacccc cggagttcgc
     5221 cgcgctgagc gcgtcccggg caggacagca tcccctttac ggtgagctcg gccggcagta
     5281 cccgccgctc gtccgtggct ggcaccgccg cgtgctgagc aacggacaga tcaccctgtc
     5341 tctctactgg ggcgatgccg aggcggggct ggacgacatc gacggacgcc agctgcgtcc
     5401 gttcgacgtc tggtttctcg acggctttgc ccccgaccgc aaccccgcca tgtggacgcc
     5461 gaccctgctc gagaaggtcg gggccctggc cggtgacgct tctacggtgg cgaccttcac
     5521 cgccgccggg cgggtgcggc gggcgctgga agctgccggg tacaacatgc gccgggtgga
     5581 ccagcggcca cacaagcgcg aaagcctggc cggtgagcgc cgccacggcg ggcggcccgg
     5641 attcgcgcca ccggctcacg ttcgggtggc gggagccggt ctggccggcg ccagcgtcgc
     5701 ccgacagctg gcggagatcg gcatcgacgt caccgtccac gaggcgggct cggccccggg
     5761 aagcggcgcc tcgggcatcc ccgcgacggt gcttcacccg cggctgctcg gtgacggcag
     5821 cgtccaggca gccttgcgct gccacgccta tctcttcagc catccttatc tggccaggtg
     5881 gctttccgat ccgacgcgag gcgttctgca gcttcccggg gagcgacagg gcgcccaagg
     5941 catcgagcgc atcgccgatc gctatgcggg caccggaagc tggctggaag tcctgccgcc
     6001 gcacagagcg gccgcggtcg ccgactggcc ggtggcttca ccgggcctgt ggttccctga
     6061 tggcagggtc gtggacacgc cggggctgac ccgggcgctg ctggaccacc cccggatcga
     6121 gctcgtgacg ggttcggcga tcaccacgtt ggatgcgccc gggaccgagg ccacggtgct
     6181 cgcctgcggg gccgactgcg cacggttcga agaagcccgg tacctggagt tatcgcccgt
     6241 cgacggccag atcgacatcc tggcatccga ccgccttccc aggttgcctt tggtgggcaa
     6301 cggctacgtg gtgcccggcg ccggcgggct cgtcgtgggc acgacgtacg agtaccggcc
     6361 ctggccggcc ggttccgcca ccgcagcaaa cctgcgtcag ctgccggagg acgtcgggcg
     6421 cgcggcccgc tggctgggtc gtgcgcgagg aacccgcgcg gtgacttcgg accggctgcc
     6481 ggtggccggg cgcctgttcg acctgaacgc gcaccccctg acgaacgcgt acgcgaccac
     6541 cggtcatggc tccatgggga cggtgttcgc gcctttcgcc ggcgccgtcg tcgcctcgct
     6601 gatcagcggc gacttctccc cgctggagcg ggatctggag caggccctgt cgtccgcgcg
     6661 gttccgcacg cgccaggcgc ggcgtggcta tcggcacggg gcgcagccgt aggccggcta
     6721 gtcgatcttg aactcgcgca cgttcatgac gctctcgatg gcgcagatgg catcgatgag
     6781 ctcgggcgcc ggctcctcgg caacgtcgat caggttgtag gccacttcgt cccggctctt
     6841 gttgagcatg tcgatgacgt tgatgttcga ctccgccagg atcgacgtca tcttgcccag
     6901 catgcccggc acgttgcggt tcgtgatcgc cagccgatag cccttgctgg gctccagatt
     6961 cacggacggg aagttgaccg agttgcggat gttgccggtt tccagaaact cgatgagctg
     7021 ctccgcggcc atcaccgcgc agttctcctc tgcctcctcg gtgctcgccc cgagatgagg
     7081 ggtgagcctg acgttggggt ggtcggtcag ccccgggacc gggaaatcgg ccacgtagag
     7141 ggcgacccgg ccatcatcca gcgctgcgcg cagggccccc gagtcaacga tgggctgtcg
     7201 ggcaaagttc agcagcacgc ttcccggccg gaagagcctg aggctctcgt cgttgatcat
     7261 ccccgtggtt tccggaagca gcggaacgtg cagggtcacg tagtcggccc gcgcaaacag
     7321 gctcggcaaa ttctccattc ggcggacctg gcttggcaga cgccaggcgg catcgaccga
     7381 gatggccggg tcgtagccca gcacctccat gccgagatcg agggccgcac gagccaccag
     7441 cgagccgatg gcgccaagcc ccacgacgcc gagtgacttg ccctgcagct cgcgcccctt
     7501 gaaccgcttt ttctcggctt cgacaagccg atcgagctcg tcttcgtcct cgatatcgcc
     7561 cagcgagctg acgaactcga tgccgcccac cacgtcccgc gcggagatca gcagagcggc
     7621 cagaaccagc tccttgaccg agttcgcgtt ggcaccgggc gtgttgaaca ccacgatgcc
     7681 cttttccgtg cagtccgcca ccgggacgtt attgaccccg gcacccgccc gcgctaccgc
     7741 gcgcaggctc tcgttgaggt cggtcggggc cagctggtgg ctgcgcagaa gaatggcgtg
     7801 cgggtccgcg atctccgagc ccacctcgta gtgctcgcgg gaaaatcttt ccagcccgcg
     7861 aacggcaatg ttgtttaatg tccgaatctt gtacatacgc tgcagccgct gtcccgttgc
     7921 ccatcacccc cggaggcggg gggcgcgtat ggtatacaaa tcctgttgcc gcgcggaagg
     7981 tccgatcagg atccggccgg aagaaagggg gtcagctccg agcagatcag ctccaccgcg
     8041 tcaggcggcg caaagccatg cagccgggcc gccgcggctt cacgttgcgc atccagcgcg
     8101 gcgatctctc gcagcagcgc ggggccgtcc agatcttcct ccagcagcac ccggctgtaa
     8161 ccctgggcca gggcaaactc ggcgttctcc agctgatcgc ccctgctggc ccgccgggaa
     8221 agggggatca gcagattcgg cttgccgaga gccagcagct catagagcgt gttggccccc
     8281 gcacgggaga ccaccaggtc tgcggccgtc aggatgtcgc cccagccctc ggtgacgtac
     8341 tcgaactgca ggtagcgcgg ttgcgctgtg cccgtgacct ttcccggccc acacacgtgg
     8401 accaccagcc agctcgccag cagctcgtcg agcacctcgc gaaccacctg attgatgacg
     8461 tccgcgccga ggctgccccc ggtgatcagc agcacgcgct cgtcttttcc cgccccgagc
     8521 cgctgccggc cccgggcgcg gtccccggcc aggagcgctt cccgcagggg tgtccccgta
     8581 tgcaccaccc gccctttgaa gccgggaaac gccgtcgagg aaaagctggt gcagacggtt
     8641 ctaaggaacg gcagggccag gcggttggcc agaccgggcg tgaggtcgga ttcgtgggca
     8701 acgacgggca cgcgccgcag ccaggcggcg aaaaccactg gaaaactgac gaagccgcct
     8761 ttggagaaca ccacgtcggg acgcagtcgg ccgagcagcc tgaatgcctg ccagaccccc
     8821 agcagcacgc gaaagacgtc gcgaagattc tcgacagaca ggtagcggcg cagcttgccc
     8881 gcggcgatgc cgtagaagtc ggcatcgacg ccttcgagca gttccgcttc cagcccgctg
     8941 ttgctgccaa cgaagcttat ccgaacgccg cggccggcaa gcgcggcgat cacggggaag
     9001 gtgggcacca cgtgaccggc cgttccgcca ccggtgaaga ccacgtggct cacaggccca
     9061 gcgcgtcctt gagcagggga atggtcacga ccctctgagc gcgcatggcc tccgcatcga
     9121 tgacttccag gtcatcgagc aggcgccgaa ccgagcggtg gctgcgcgca agccagtagc
     9181 cgagcaccgc atcggacagc tccagccccc gctccttggc caaccgccgc aggagacgta
     9241 ccttgccctc gtcgttcagg ggcctgacct cgtatgcggt caggctcgac aggcgcgaag
     9301 cgaggtcggc gagccggaag ccgcatcgag ctgccggacc cgccgccccg acgatcaacc
     9361 tggctcgccg cgcgaacagc cgctcgtaca tcgccatcag cgggctctcg agctgcagat
     9421 caccggccca ggcctcgacg tcgtcgaggg ccagaagatc atagaaatcc agtccttcca
     9481 ggacggcggg atcacgctcg acggccccca ggggcagata ggcaacccgc gcgccgcgcg
     9541 ccgcgtgcca atgacacacg ccatgcagca gatggctgac acccaccgcc ccgccgcccc
     9601 agagccatag acccgagaag acgctccgat ccggctccgc ctcgagtcgc tggacgacct
     9661 cggcattttc tccgatctcg aagttgccca gcacgaatcg ctgattgatg ggaaacggca
     9721 gcgacagctg ctgtcgcgga cccgtcatcg tcccggccga ctcacagcgc ggccttggcc
     9781 cggcgcacgg ctcgcaggct ccaagtgatc acgtagtcca gccccgacgt gagcccgagg
     9841 gcggcaagga cccagaagcc gtacggatcg gccagcagcg ccgccgcccc gctgatccag
     9901 tcgaagccgc attgcgcgat gagcagcagc agaagcagga cgatctgcac ggcggtattc
     9961 gctttgctga ggtaggtggg ggcaaattcg atggtctcga aaagcaggcg gtaggcaagg
    10021 gccccgccca ggatgatcac gtcgcgaccc aggacgatca gggcaaccca gatcggtatg
    10081 atccccttga gggtgaagat cacgaacagc gtgcccacca gcagcttgtc cgcaaccgga
    10141 tccatggcgg cgccaaagcg gctcatccaa ccgaagtgtc gggccagcca tccgtccagc
    10201 gcatcggacg cgctggcgac cgccatcaga atcagcgcct cccggaccga cccgtaccag
    10261 agcaacaggc cgacgggagc gaccatcgcg atgcgcgcca cggtgatgag gttggggatc
    10321 tgggccgtcg tgaacattca gcgtcgccag agcagctcga cgtccgccga gcccggcacc
    10381 gcgcccggca gctcggcgag ccgccggtcg aagcgaaagt tcgcaagcag ctgttcgatg
    10441 tcggcgcgag tggtgagcac cagtccgagg cgatcaccct tgacgccggc cactgcgatg
    10501 tcgtcgacga attccagctc tgccagatag ctgagcagct ccccgtagtc tctggccgag
    10561 ccgacgccgg acacgccgac gagcaccggc cgggcaaacc tgccgaggac ggcatgccgc
    10621 tgcgcgagct cgtcggcaag aaagtccacg cccgcctcca ccagccggac ggcattttcg
    10681 gcactcatcg aaaacggatg cagatcgcca tcgagccaga aatcccatgc gcccgtccac
    10741 tgctcggcgc cgatctgctg cagacgcccg acgaggacga cgtccgcgcc gtagcgggcc
    10801 gaggccggct cgagcaccgt agatacccgc ccccaaaccg cggcgggatc cacgagcata
    10861 ctgtcctgaa ggtccatcag cggcagcgac accagcaccc cgcggtcccg ggcccgcgcc
    10921 gacagctcgg aagcgaggcc ggccgcatct tccggtgaga ggatggaacg tgccccgccg
    10981 cggtccagca cgatccaggc cgccacccga ggacggttgg aacgccagac gggcagctcg
    11041 gcatccttga ccagctgcag cagagatccg cgctcgaact gcacgatgag cttgagctct
    11101 tcgatcggcc gtccgtccgc gcccacaccc tccacctcga ggtagcggaa ctggttgtag
    11161 tagcgccccg gagccgacag cgcttcccga accgcctcca cgcggggcac gctggtcaga
    11221 cccgtcacgc gggtgagcac ctgcagcagg gcttcgccgg aggcgtcgag ccttgcctcg
    11281 cggctctggt tctccaccgg gacctctacc tcgtagagcc agggaacgac gtccgcgacg
    11341 gcgccgcccg tcagcaggaa cgcaagcgtg actttcagga ggtgcgatcg tgctgagacc
    11401 accgtgggca ccgcggctcc gacatcagta cggcgtcgaa gtttacccaa ttcgccaggt
    11461 caggtctggt aaactccgcc gccatcaagg ggcggcaaca gggcgaggat tcaacgtgac
    11521 caagggtctc acctaccgtg acgccggggt ggacatcgac gccggcaacg agctcgtacg
    11581 ccgaatctca gccgcctgca aagcgacgca gcgacccgag cagctcaccg gcctcggcgg
    11641 cttcgcggcc ctggccgcaa tgcccgagcg ttaccggcaa ccggtcctgg tgacgggcac
    11701 cgacggtgtg ggcaccaagc tcaagctcgc catcgatcac gaccggcacg accaggtcgg
    11761 ccaggacctg gtggccatgt gcgtgaacga cgttctggtg accggtgccg agccgtttct
    11821 tttcctggac tactacgcca ccggccgact ggacgtggac gtggcggaac gggtcatcaa
    11881 aggtatcgcc catggctgcg agctgtccgg ctgc
//
LOCUS       DBNHLM010000224         2764 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_749824, whole genome shotgun sequence.
ACCESSION   DBNHLM010000224 DBNHLM010000000
VERSION     DBNHLM010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_749824"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="AC16QF_10375"
     CDS             <1..387
                     /locus_tag="AC16QF_10375"
                     /inference="COORDINATES: protein motif:HMM:NF028441.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HIR6449005.1"
                     /translation="YEDLTVQEIEKFSYRFLGQGQANGIDCFVVERTPRDDFSGYSRQ
                     QVWIDQDELRVLRIEYFDRRDSLLKTLTIDQYARYEDQFWKAGRMYMTNHQTDRSTEL
                     FWRNYRFRTGLMDERDFSTNSLLRVR"
     gene            450..1658
                     /locus_tag="AC16QF_10380"
     CDS             450..1658
                     /locus_tag="AC16QF_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449006.1"
                     /translation="MAAQPASTDGIEPSSADHSLDLDLGLQLRAFSKSGLDGQSRTQG
                     SVSAQLEYYRAWNEDRSSLTFTPFVRVDGEDDERTHFDVRELFWSRVGPDWDLHLGAK
                     QVFWGVTEFKHLVDVINQTDLVENIDGEDKLGQPMAHLSVVRDWGILDFYLLTGFRER
                     TFPGSDGRLRPPLPVDDDARYESGAGRWRVDGAVRWQRQLGRLTLGIHHFSGTSRAPV
                     LIPETGPAGQGVLRPFYPVIDQTGLDAQAIVGDWAWKLEAVSSSGYEGRYSAFNAGFE
                     RSLVGVLGGRGDLGLVVEYMFDDRDERAFDTLFEHDLALGTRWQMNDVADTQALIGVI
                     WDVKTDEYVVKLEGSRRLGDTWTLLVEGRIFGGSERPMPGVPPSTDPNNRAWPLERDD
                     FLQLELTRYF"
     gene            1675..2742
                     /locus_tag="AC16QF_10385"
     CDS             1675..2742
                     /locus_tag="AC16QF_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6449007.1"
                     /translation="MLESLRDFLSQNTWVVEVFAIVFATALVRYAVRRAFERLAGRLE
                     QTHNIYDDALFDAARGPVGVGIWVLGVSWAAEVAGAVGGAEIFDYVDEVREVAIIWLL
                     VWFALRFIRFMEVHLADSSYREEPVDETTAAAVGKLLRAAAIITGVLMAMQEFGFSIA
                     GVLAFGGVGGIAVGFAARDLLANFFGALMIFLDRPFSVGDWVRSPDREIEGTVEDIGW
                     RLTRIRTFDQRPLYVPNSIFTNLAVENPSRMRNRRIYETIGVRYEDVGVLRQIVADVE
                     SMLREHPDIDHGRTLMVNFVEFGPSSLDFFVYTFTKTTVWAEFHAIKQDVLFRIADII
                     EEHGAEIAFPTQTLHVVQPES"
BASE COUNT          450 a          852 c          921 g          541 t
ORIGIN      
        1 tacgaggacc ttaccgttca ggagatcgaa aagttcagct accgcttcct tggccagggg
       61 caggctaacg ggatcgactg cttcgtggtg gagcgtaccc cgcgcgacga cttctcgggc
      121 tactcgcggc agcaggtctg gatcgatcag gacgagctgc gcgtgctgcg gatcgagtac
      181 ttcgaccggc gggacagcct gctcaagacc ctgaccatcg atcagtatgc ccgttacgag
      241 gatcagttct ggaaggccgg ccggatgtac atgaccaacc atcagacgga ccggagcacc
      301 gagctcttct ggcgcaacta ccggttccga acggggctca tggacgagcg cgatttttct
      361 accaacagcc tgctgcgggt gcgatgactt gccaagggcc ccgtccctgg tgcctggcag
      421 ctgcgctgct cgctgccggc gggatcagtt tggccgcaca gccagcctct accgacggca
      481 tcgagccgtc ctccgctgat cactcgctgg acctcgacct gggacttcag ctgcgggctt
      541 tctcgaagag cggcctcgat ggccagtccc ggacccaggg atcggtcagc gctcagctgg
      601 agtactaccg tgcctggaac gaggatcgat ccagcctgac gttcacgccg ttcgtcaggg
      661 tcgatggcga ggacgacgag cgcacgcact tcgacgtgcg ggaactgttc tggagccgcg
      721 tgggtccgga ctgggacctt catctgggag ctaagcaggt tttctggggc gtcacggaat
      781 tcaaacacct ggtcgacgtg atcaaccaga ccgacctggt agagaacatc gacggcgaag
      841 acaagctggg acagcccatg gcccacctct cggtcgtgcg cgactggggc atcctggact
      901 tttacctgct gaccggcttt cgcgagcgga cttttcccgg ctccgacggg cgtctgcggc
      961 cacccttgcc ggtggatgac gatgcccgct acgagtccgg cgccgggcgc tggcgggtcg
     1021 acggcgccgt tcgctggcag cgtcagctgg ggcggttgac cctcgggatt catcatttct
     1081 cggggacgag ccgcgccccc gtgctgattc ccgagaccgg cccggccggg cagggcgtgc
     1141 tgcgcccgtt ctatccggtg atcgatcaga ccggtctgga tgctcaggcc atcgtcggag
     1201 attgggcctg gaagctcgag gcggtttcca gcagcggtta cgaaggtcgc tactcggcgt
     1261 tcaacgccgg cttcgagcgc tctctggtcg gcgttctcgg gggacgcggc gacctgggtc
     1321 tggtggtcga gtacatgttc gacgatcgcg acgagcgagc tttcgatacg ctcttcgagc
     1381 acgacctggc gctcggcacc cgctggcaga tgaacgacgt ggctgacacc caggcgctga
     1441 tcggcgtgat atgggacgtc aagacagacg aatacgtggt caagctggaa ggtagccgcc
     1501 gcctgggcga cacttggacc ctgctggtgg aggggcgcat cttcgggggc tccgaaaggc
     1561 cgatgccggg cgtgccgccc tccacggatc cgaataaccg tgcctggccc ctcgagcggg
     1621 acgactttct tcagctcgag ctgacccggt acttctgaca ggcggcaggg cctcatgctg
     1681 gaatcgttgc gggattttct cagtcagaac acctgggtcg tggaggtgtt cgccatcgtt
     1741 ttcgccaccg cgctggtgcg ctacgcggtg cgccgggcat tcgagcggct cgccgggcgt
     1801 cttgagcaga cccacaatat ctacgacgac gccctgttcg atgccgcgcg gggaccggtt
     1861 ggcgtcggga tctgggtgct cggtgtttcc tgggccgccg aggttgccgg ggcggtgggc
     1921 ggggccgaga ttttcgacta cgtggacgag gtgcgcgagg tcgccatcat ctggctgctc
     1981 gtgtggttcg ccctccggtt cattcgattc atggaggtgc atctggccga ctcgtcttat
     2041 cgggaggagc cggtagacga aacgaccgct gccgccgtgg gaaagctcct gcgggccgcc
     2101 gccatcatca cgggcgttct gatggccatg caggagttcg ggttcagcat tgctggcgtc
     2161 ctcgcctttg gcggtgtcgg cggcatcgcg gtgggtttcg ccgcgcggga cctgctggcc
     2221 aactttttcg gcgcgctcat gattttcctg gacaggccgt tctccgttgg tgactgggta
     2281 cgttcccctg accgtgagat cgagggcacg gtggaggaca taggctggcg gctgacccgg
     2341 atccggacat tcgatcagcg gccgctctac gtgccgaact cgatattcac gaatctggcg
     2401 gtggaaaacc cgtcgcggat gcgcaatcgg cgcatctacg aaaccatcgg cgtgcggtac
     2461 gaggacgtcg gcgtgctgcg gcagatcgtg gccgacgtgg aatccatgct ccgagagcat
     2521 ccggacatcg accacggcag aacgctgatg gtcaacttcg tcgagttcgg gccttcctcc
     2581 ctcgacttct tcgtctacac gttcaccaag accacggtct gggccgagtt tcacgccatc
     2641 aagcaggacg tgctctttcg catcgccgac atcatcgaag agcacggcgc ggagattgcc
     2701 ttccccaccc agacgctgca cgtggtgcag cccgagtcgt gactgctcgg gtccccactg
     2761 agat
//
LOCUS       DBNHLM010000225        18050 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_451010, whole genome shotgun sequence.
ACCESSION   DBNHLM010000225 DBNHLM010000000
VERSION     DBNHLM010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 18050)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18050)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18050
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_451010"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="AC16QF_10390"
     CDS             <1..476
                     /locus_tag="AC16QF_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR6449008.1"
                     /translation="FGGRIAEQLIFGKDSVTTGASNDIQRATEIARNMVTKWGLSEKL
                     GPLKYDEDDGEVFLGMSAGTKTKPIGDKTKDAIDKEIRDIIDTSYTTAKQLLEDNIGK
                     LHKMAEALVEYETLSSDQIDDIMAGAKPRAPGNPSPPSGKAPSGESPIGDPAGEH"
     gene            451..1326
                     /gene="folP"
                     /locus_tag="AC16QF_10395"
     CDS             451..1326
                     /gene="folP"
                     /locus_tag="AC16QF_10395"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR6449009.1"
                     /translation="MAIPPGSTDSPAAVFCRGRKLELEHPRIMGVLNVTPDSFSDGGR
                     LMSRGRVDVERVVAAAHAMIAEGAAILDVGGESTRPGSLPVAVADECSRVIPVIERLS
                     ELDTIVSVDTSKPEVARLALQAGCHLVNDVTGLRDPGMRKVVAEADAAACVMHMRGEP
                     RSMQNEPVYKDVVAEVRAFLEERVGACDEAGIDRQRLLLDPGIGFGKRLEHNLALLRN
                     LEGLRFAGLPVLVGVSRKSMLGALTERPVTDRDAMSAVAAALAVEHGASVVRTHDVGL
                     TADALKLVTAWHGHG"
     gene            1374..2708
                     /gene="glmM"
                     /locus_tag="AC16QF_10400"
     CDS             1374..2708
                     /gene="glmM"
                     /locus_tag="AC16QF_10400"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003221540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR6449010.1"
                     /translation="MEKKYFGTDGIRGRVGDAPITPEFCLRLGWAAGRVFARSGKCHV
                     LIGKDTRISGYMLESVLESGLVSAGANVSLLTVMPTPAVAYLTRTLRADAGIVISASH
                     NPYCDNGIKFFDGSGNKLPDAVELEIEAELNREMACVDSDRLGKADRLVDAPGRYIEF
                     CKASVDRALRLKGLKIVLDCAHGATYHVAPRVFEELGAEVSVCGANPNGTNINDNCGA
                     TAPARLAERVAEEGAHLGIAFDGDGDRVIMVDEQGDIVDGDELLYVIAKHRATQGTLH
                     GGIVGTLMTNYGLERALAGLNVPFERAAVGDRYVLEMLRERGWTLGGEASGHIVCLDL
                     TTTGDAIVAALQVLTAIVQSGQPLHQLKQGMSKLPQVMVNVSAPDPSRAADERALQLA
                     VKDKSRLLAGRGRILVRRSGTEPVIRVMVEGEDHAEVSAIADDLAAAVQQEG"
     gene            2798..3559
                     /gene="tpiA"
                     /locus_tag="AC16QF_10405"
     CDS             2798..3559
                     /gene="tpiA"
                     /locus_tag="AC16QF_10405"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008017141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR6449011.1"
                     /translation="MSPRRPLVAGNWKMNGSSEFVRSFAAELMARVVEDAVSVVVFPP
                     AAYLGALVRAFEGSAIEVGGQNAHMESSGAFTGEVAPEMVADVGGRWLLVGHSERRQY
                     FGESDDLIADKYAAALRACLAPVLCVGETLEEREAGEAERVVCRQLAAVTERLDIAEV
                     ARGVVAYEPVWAIGTGRTATPGQAQEMHRVIREALSERGAKLAEGMRILYGGSVNADN
                     AGELMAQPDIDGGLVGGASLSADGFAAIVAAAAGR"
     gene            3645..3974
                     /gene="secG"
                     /locus_tag="AC16QF_10410"
     CDS             3645..3974
                     /gene="secG"
                     /locus_tag="AC16QF_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR6449012.1"
                     /translation="MLLVVDAIALAALVLLQQGRGADVGAAFGSGSSNTMFGSAGSAS
                     FLTKLTVWLAIGFFLISFGLAYTAKERAAIIDEVGVPSVAPAEETVVPEQLTPEPESV
                     DSDIPDV"
     gene            3983..4069
                     /locus_tag="AC16QF_10415"
     tRNA            3983..4069
                     /locus_tag="AC16QF_10415"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4017..4019,aa:Leu,seq:gag)
     gene            4189..4265
                     /locus_tag="AC16QF_10420"
     tRNA            4189..4265
                     /locus_tag="AC16QF_10420"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4223..4225,aa:Met,seq:cat)
     gene            4527..4997
                     /gene="rimP"
                     /locus_tag="AC16QF_10425"
     CDS             4527..4997
                     /gene="rimP"
                     /locus_tag="AC16QF_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR6449013.1"
                     /translation="MNAKEKQIEALLTPTVEGLGCQIWGVEYASQGRHSRLRVYIDRE
                     DGVSVDDCERVSRHVSDVLDVEEVISGGYTLEVSSPGMDRVLFKAEQYAANVGQHVDV
                     RLVYPFEGRKRIVGVLAGLEEDDSCGHVIVQVDDEQYLLPLESVQRTRIIPQYD"
     gene            5013..6530
                     /gene="nusA"
                     /locus_tag="AC16QF_10430"
     CDS             5013..6530
                     /gene="nusA"
                     /locus_tag="AC16QF_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR6449014.1"
                     /translation="MSKEMLLVVDTVSNEKGVPKDVLFGAVEAALATATKKRYDDDAE
                     FRVAIDRESGEYETFRVWTVADPEALEEEEEWNPAAQLTVEEARERDANLQLGDVIEE
                     PVESVGFGRIAAQTAKQVIVQKVREAERAQVVEAYLPRVGELVNGTVKKLGRDSVIVD
                     LGNNAEGLLTREHLIPRETFRVGDRLRALLLEVNPDNRGPQLMLTRTSPKMLIELFKL
                     EVPEIAEDVIEIRGAARDPGSRAKIAVKTNDGRIDPVGACVGMRGSRVQAVSGELAGE
                     RIDIVLWDDNPAQLTVNAMAPAEVAAIVLDEDTHAMDIAVTEDNLAQAIGRGGQNVRL
                     ASDLVGWSLNIMTEEDAAAKQEEEAVRYIEEFMRALEVDEDVATVLVEEGFTSIDEVA
                     YVPIEEMTAIEGFDEEIVEELRNRAKDALLTKAIASEELLGEKEPADDLLNMEGMERR
                     LAFQLASNDIITMEDLAELSVPDFIEIVPEVDEETAAALIMTARAPWFEEAEAEG"
     gene            6556..9090
                     /gene="infB"
                     /locus_tag="AC16QF_10435"
     CDS             6556..9090
                     /gene="infB"
                     /locus_tag="AC16QF_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR6449015.1"
                     /translation="MSEVTVKQLADTVGAPVDRLLKQMQDAGLPHDSDDAAVTEEEKQ
                     TLLAYLKRSHGSDDAAPKKITLKRKTVGTLKSGQGRTGRTVNIEVRRKRTYVKRDEAE
                     TAGAAAEAPAKPASQSELEAQRIREQELARKEAEEETRRKEAERKAEEEKRVEEIKRQ
                     EEADLQARETKPGEAPAEDSAAKAAAAAAEAQAKAQASERGGKRVRGKERERPGEKRD
                     EFGDGKGRRRELSLKTQRRGKRRQPAPMPVEQQGGEFRPTDFIAREVEIPDIITVGEL
                     AQRMTVKAGEVIKALMGMGVMATINQSVDQDTATLIVEELGHKPKPIHEDAMEQALEA
                     SLQIEGDAAKRAPVVTVMGHVDHGKTSLLDYIRKTRVTSGEAGGITQHIGAYSVDTPH
                     GKISFIDTPGHAAFTAMRARGARVTDIVILVVAADDGVMPQTEEAIQHAKAAGVPVVV
                     AINKMDLEGADPDRVTNELAAKEVVPEDWGGDTQFVKVSAVSGEGIDELLEALLLQAE
                     ILELTAVEDAPGRGVVIESRLDRGRGPVSTVLVQNGTLKQGDVIIAGECHGRVRAMLN
                     DKGEQVKAAGPSVPVEVLGINGTPAAGDDFSVATDERTARELAEFRRDKSQEQRVATQ
                     HAAKLENMFANVGAGEKRVLKMVLKADVRGSLEAITQALTEMGNEEVAVNVLGSGVGG
                     ITETDANMALTYGAAVFGFNVRADNSAKAIIEREGLDLRYYSVIYELLDDVKQVLSGM
                     LSPEVREEIVGIAEVRDVFRSPRFGQVAGCMVTEGTVYRNKPIRVLRDNVVIYEGELE
                     SLRRFKDDVNEVRSGTECGIGVRNYNDVRVGDLIEVFDTKEVAREL"
     gene            9129..9548
                     /gene="rbfA"
                     /locus_tag="AC16QF_10440"
     CDS             9129..9548
                     /gene="rbfA"
                     /locus_tag="AC16QF_10440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR6449016.1"
                     /translation="MREFGRDLRVADFVRDELAKIIQREMRDPRVGMVSVNEVTVSRD
                     LAFADVYVSSIEARTAKEREELIGVLNKAAGFFRSELAKRHSMRTTPKPRFHYDEMVE
                     RGPELEALIDFAVGSDTRDEDPGTEDELGEGEGRGRG"
     gene            9541..10461
                     /gene="truB"
                     /locus_tag="AC16QF_10445"
     CDS             9541..10461
                     /gene="truB"
                     /locus_tag="AC16QF_10445"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR6449017.1"
                     /translation="MGRRGRGRDVSGVLVVDKPAGWSSNDAVQKAKRLFNARKVGHTG
                     ALDPLATGVLPLCFGEATKFSQYLLSSDKKYEVRIKLGVATDSGDADGNVVAEREVDD
                     VTPARVEEALRFFRGEIDQVPSMFSAIKHQGQPLYKLARQGIEVERQARRVTVYSNEL
                     ISLEGDVVELHIHCSKGTYVRTIAQELGEVLGCGAHVSALRRTMAGPYGESDLVTFAE
                     LESSLEAEGTLDRFLQPISSTVREWPEVRLTDNTAYYLRQGQPVMVPHAPARGWVRLA
                     EIVGEDSTRFLGVGEVLDDGRVAPRRLVVG"
     gene            10527..10796
                     /gene="rpsO"
                     /locus_tag="AC16QF_10450"
     CDS             10527..10796
                     /gene="rpsO"
                     /locus_tag="AC16QF_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR6449018.1"
                     /translation="MALSAESKADIIKEYQIEEGDTGSPEVQVALLTHNINSLQEHFR
                     SHNKDHHSRRGLIRMVSQRRKLLDYLRSKDASRYSELIKRLGLRR"
     gene            10866..12965
                     /gene="pnp"
                     /locus_tag="AC16QF_10455"
     CDS             10866..12965
                     /gene="pnp"
                     /locus_tag="AC16QF_10455"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019410393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR6449019.1"
                     /translation="MNPVTKEFQFGDQTVVLETGRIAKQATGAVLVTMGDTVVLCTVV
                     GAKSARPGQDFFPLTVNYQEKTYAAGKIPGGFFRREGRPSEKETLTCRLIDRPIRPLF
                     PKGFLNEVQVICTVMSTDKDQDPDIAALLGTSAALAISGLPFNGPIAAARVGYLDGRY
                     LINPGYAALESSLLSMVVAGTESAVLMVESEAKELTEDEMLGGVLYAHQEMQVAIEAI
                     KELAATAGKPKWDWSPAESNESLVAEVSDKVRADLGNAYRITDKMERQARVSELRAAL
                     VGDLGGGNEPSFASDNVAGAFGKVEKDIVRQRVLDGEPRIDGRDLRTVRPISVEVGFL
                     PKTHGSALFTRGETQAIVVATLGTMRDAALIDALEGTYKDNFMLHYNFPPYSVGEAGF
                     MTGPKRREIGHGRLARRGVQAMLPDVDDFPYTIRVVSEITESNGSSSMASVCGTSLAL
                     MDAGVPMKAPVAGVAMGLVKEGNKYAVLTDILGDEDHLGDMDFKVAGSANGICALQMD
                     IKIDGITEEIMEIALDQAREARLHILGEMNKVLDRSRETLSENAPSMVVLKVHPDKIR
                     DIIGKGGATIRGIVDETGADIDIEDDGSVRIYAEDAASKDAAVARIEEITAEAEIGKV
                     YTGKVARIVDFGAFVNILPGKDGLLHISQIAEERVENVTDYLQEGQDVQVVVLDIDQR
                     GRIKLSMKEVANYAQSA"
     gene            complement(13015..14958)
                     /gene="acs"
                     /locus_tag="AC16QF_10460"
     CDS             complement(13015..14958)
                     /gene="acs"
                     /locus_tag="AC16QF_10460"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetyl-CoA synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HIR6449020.1"
                     /translation="MSETHIVPVPQSVLDVCYINDDQYEAMYERSITDPEGFWSEQAD
                     KFVAWYEKPHQVREADMVRGNISWFAGGKLNIAYNCIDRHLPDRASQTAIIWEGDDPA
                     NDKTITYQELHDAVCQLANGLRSRGVGKGDRVCIYMPMVPEAAYAMLACMRIGAIHSV
                     VFGGFSPQSLKDRILDSDCRTVITADHGVRGGRLVPLKENTEAALKDCPNVHTVVTVK
                     RTGNEIPWNPDRDIWYEDLVADQPRTAEPEVMDSEDPSFILYTSGSTGKPKGVQHGTA
                     GYLLFAAMTHKYVFDYHDGDIYWCTADVGWITGHSYIVYGPLANGATTIMFEGVPTYP
                     DASRCWQVVDKHKVNIFYTAPTALRQLMGQGDEYVTQTSRASLKLLGTVGEPINPEAW
                     QWYHKVVGEERCPIVDTWWQTETGGIMISPLPGATALKPGSATRPFFGVELGLMDENG
                     RLIEEQEASGNLVITNTWPGQIRSVYGNHQRVIDTYYSQYPGYYFTGDGARRDADGYY
                     WITGRVDDVINVSGHRIGTAEVESALVLHEAVAEAAVVGFPHPVKGEGIYAYVTLMVG
                     NNEDPDQLRGELEALVRSEIGPFARPEFIQYAPGLPKTRSGKIMRRILRKIAADDLEN
                     LGDTSTLADPGVVDDLVHNRANR"
     gene            complement(14995..16557)
                     /gene="pgi"
                     /locus_tag="AC16QF_10465"
     CDS             complement(14995..16557)
                     /gene="pgi"
                     /locus_tag="AC16QF_10465"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR6449021.1"
                     /translation="MRPDDLAPWKSLTRLSADSIKQPLSDWLRSPERFARLSFEVGEL
                     LVDLSKQRLNEEIVDALLALAGAARLDRRIDDLFEGAQVNPTEQRPALHTALRAPLAS
                     RPAAYGEAVETTLTRMLDFAERVRSGVWRGATGKAIKSVVHIGIGGSHLGPELAVRAL
                     EGAKGAGPSVRFVANIDRSALDTALTGLDPETTLFIVASKSFTTLETRVNADSARAWF
                     LERMADLDALKHHFVAITENVEAARAFGMGPEQIFPMWDWVGGRYSLWSAVGLPVAIS
                     AGADAFRELLAGAHAMDEHFRSSPLSGNVPVLMALAGVWNYNFLGVSNHAILPYSARL
                     GLLPSYLQQLEMESNGKSVDLEGEAVTVHTAPIIWGGEGTNGQHAFHQLLHQGTRSFT
                     ADFIMVGQPDPDDVHGRWLLANGLAQSQAMMAGQDADDAHRRVAGDHATTTIVMRQLN
                     PFSLGSLLALYEHKVFCQGVIWNINSFDQFGVELGKTLSVPIFQQLGGNSAMTQDAST
                     RGLVELLRGTTG"
     gene            complement(16682..17434)
                     /locus_tag="AC16QF_10470"
     CDS             complement(16682..17434)
                     /locus_tag="AC16QF_10470"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIR6449022.1"
                     /translation="MSIIKASDQLIRSMTPPAQHSLAIETTAALGQKLEGKSLPRYIV
                     IEGPIGVGKTTLANKLAEAFHYPILLEPAAQNPFLNSFYRDGRRNALPTQLFFLLHRA
                     QQIANLDHADLLGPTLVADFLMEKDDLFARMTLDEDEYALYRQIHETLDIDPPKPDLV
                     IYLQAPVDVLRTRIRDRGIDYERDIEAGYLESLARAYTEFFHFYDAAPLLVVNAASID
                     FANNAVHFESLLDQILAMDGLRQYFNPNPTLL"
     gene            complement(17449..>18050)
                     /locus_tag="AC16QF_10475"
     CDS             complement(17449..>18050)
                     /locus_tag="AC16QF_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024040.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HIR6449023.1"
                     /translation="KLMMSGAAGAAWKAIAGTPLRAALFPGTPPDSRLVELAMANTDQ
                     RIASDLPVTPGFLLAVLMWHDFQARCNALTDERKPAEARHQAASETLAAQQAVTSMPR
                     RFSQFVRDVWSLQPRLEARLPRQIDRIVQHPRFRAAYDFLVLRAESGEQATDSEPLDD
                     AAQWWTTFQQADQATRADMTEARLAVQPPRKRRRRRRRG"
BASE COUNT         3400 a         5316 c         6175 g         3159 t
ORIGIN      
        1 tgttcggcgg acgcatcgcc gagcagctca tcttcggcaa ggacagcgtg acgaccggcg
       61 ccagcaacga tattcagcgc gcgacggaaa ttgcccggaa catggtcacc aagtggggcc
      121 tgtcggagaa gctgggacct ctgaagtacg acgaggacga cggcgaggtg ttcctcggca
      181 tgtccgcggg cacgaaaacc aagccgatcg gcgacaagac gaaggacgcg atcgacaaag
      241 agattcggga tatcatcgac acctcctaca cgacggccaa gcaactgctc gaggacaaca
      301 tcggcaagct ccacaagatg gccgaggcgc tggtggagta cgagaccctc tccagcgatc
      361 agatcgacga catcatggcc ggcgccaagc ctagggcgcc ggggaacccg tctccgccct
      421 cgggcaaagc gccctccggg gaatcgccca ttggcgatcc cgccggggag cactgacagc
      481 ccggccgcgg tattctgccg cggccgaaag ctcgaactcg agcacccccg aatcatgggg
      541 gtgctcaacg tcacccctga ttccttctcc gacggcgggc gtctgatgag ccgtggccga
      601 gtggacgtgg agcgggtggt cgcggcggcg cacgcgatga tcgccgaggg cgccgccatc
      661 ctggacgtgg ggggcgaatc gacccgcccg ggctccctgc cggtggcggt agcggacgag
      721 tgctcgcgtg tgatccccgt catcgaacgc ctgagcgagc ttgatacgat cgtctccgta
      781 gatacgagca agccggaggt tgcgcgcctg gccctgcagg cgggatgtca ccttgtgaac
      841 gacgtgaccg gtctgcggga tcccgggatg cgcaaggtgg tggccgaggc cgatgctgcc
      901 gcctgcgtca tgcacatgcg aggcgaaccg cggtccatgc agaacgagcc cgtctataag
      961 gatgtggttg ccgaagtgcg ggcattcctg gaggagcgcg taggcgcttg cgatgaagca
     1021 ggcatcgaca gacagcgctt gcttctggat ccagggatag gattcggcaa gcgtctcgag
     1081 cacaatctgg cccttctgag aaacctcgag gggctgcggt tcgccggttt gccggttctc
     1141 gtgggcgttt cgcgcaagag catgctcggt gcgctcaccg agcgtccggt gacggaccgg
     1201 gacgcaatga gcgcggtggc agccgcgctc gccgttgagc acggcgccag cgtggtgagg
     1261 acccacgacg tcggcctcac cgccgatgcg ctgaagctgg tgacggcctg gcacgggcac
     1321 ggatgaagac cgggccttgg tctggctttg gttttggtaa ggacgcgctt gggatggaaa
     1381 aaaagtactt cgggactgac ggcattcgtg gtcgggttgg cgacgctccc ataacacccg
     1441 agttctgtct gcgtcttggc tgggcggcgg gcagggtgtt cgcgcgcagc ggcaagtgcc
     1501 acgtgctcat cggcaaggac acgcgcatat cggggtacat gctcgagtcc gttctggagt
     1561 ccggtctcgt ttccgccggg gccaacgtca gcctgctcac cgtcatgcct acgccagccg
     1621 ttgcgtatct gacgcgcacg ttgcgggccg acgcgggcat cgtcatcagc gcttcgcaca
     1681 atccctactg cgacaacggc atcaaatttt tcgacgggtc gggaaacaag ctgccggatg
     1741 ccgttgagct agagatagaa gccgaactga atcgcgaaat ggcgtgcgtg gactcggatc
     1801 gcctcggcaa ggcggaccgg ctcgtcgatg cgccggggcg ctatatcgag ttctgcaaag
     1861 ccagcgttga ccgggcgctg cgcctgaaag gcctcaagat cgtgctcgac tgtgctcacg
     1921 gcgcgaccta tcacgtcgct ccccgcgtgt tcgaggagct gggcgcggag gtcagcgtgt
     1981 gcggggcgaa ccctaacggc acgaacatca acgacaactg cggcgccacg gctcccgccc
     2041 ggctcgccga gcgggtggcg gaggagggcg cccatctggg aatcgccttc gacggagacg
     2101 gcgaccgggt gatcatggtc gacgagcagg gggacatcgt cgatggagac gagttgctgt
     2161 atgtgattgc caagcaccgg gcgacgcagg ggacccttca tgggggcatc gtcggaaccc
     2221 tgatgaccaa ttacggcctc gagcgcgcgc tggccgggct caacgtgccg ttcgaacggg
     2281 ccgccgtagg cgaccggtac gtgctggaga tgctgcgcga gcgcggctgg acccttggcg
     2341 gtgaagcgtc gggacacatc gtgtgtctgg atctcacgac cacgggcgat gccatcgtgg
     2401 cagccctcca ggtcctgacc gccatcgtcc agagcgggca gccgctgcac cagctcaagc
     2461 agggcatgtc caagctgccc caggtcatgg tcaacgtttc tgcacccgac ccgtcccgtg
     2521 cggcggatga gcgggcgctg cagctggcgg tgaaggacaa gagccgcctg ctcgccgggc
     2581 gtggccggat tctcgttcgg cgttcgggga cggagcccgt catccgggtc atggtcgaag
     2641 gcgaagatca cgccgaggtc tctgccattg cggacgatct tgccgccgcg gtccagcagg
     2701 aaggctgaag ccttgcaagc tgctggcgcg ccgggtcgcg gcgttgtctg cgtcagcccc
     2761 aaagtataag attgcgcgcc cttaggtgga ggccggcatg tccccacgta ggccgctggt
     2821 cgcgggcaac tggaaaatga acggctcttc cgagttcgtc cggagtttcg cggctgaact
     2881 catggcccgg gtcgtggaag atgcggtgag cgtagtcgtg ttccctcccg cggcctatct
     2941 cggcgcgctg gtccgggcct tcgaagggtc tgccatagag gtcggtgggc agaatgcaca
     3001 catggagtcc tcgggggctt tcaccggcga ggtcgccccg gaaatggtgg ccgacgtcgg
     3061 gggtcgctgg ctgttggtcg gtcattccga gcggcgccag tatttcggcg agtcggacga
     3121 cctgatcgct gacaagtatg ccgccgcgtt gcgagcgtgc cttgccccgg tgctatgcgt
     3181 gggcgagacg ctcgaggaac gcgaagccgg ggaggccgag cgggtcgtct gcaggcagct
     3241 cgccgccgtc acggagcgat tggacatcgc cgaggtggcc cggggcgtag tggcttacga
     3301 gccggtatgg gccatcggca cgggtcgcac ggcgacgcct ggccaggccc aggagatgca
     3361 tcgggtcatt cgcgaagcgt tgagcgagcg cggtgcaaag ctggcggaag ggatgcggat
     3421 tctctacggt ggcagcgtaa acgccgataa tgccggcgag ctgatggccc agccggatat
     3481 cgacggcggg ctggtcggag gcgcatcgct cagcgcggac gggttcgcgg cgatcgttgc
     3541 tgcggcggct ggaaggtgac ggaacagtgg gcctgctccg gggcaggcgg tgagcagaaa
     3601 aatcggtaac gtgccacatg acaacttttg aaacgattct gttgatgctg ctcgttgtgg
     3661 acgccatcgc gctggcagcg ctggtgctgc tgcagcaggg ccgtggcgcg gacgtaggcg
     3721 cggcattcgg cagcgggtcc tccaacacca tgtttggcag tgccgggtcg gcttcgttcc
     3781 tgacgaaact gacggtgtgg ctggccatag gctttttcct gatttctttc gggctcgcct
     3841 acaccgccaa ggagcgcgcg gccattatcg acgaggtcgg ggttcccagc gtggctcctg
     3901 ctgaggaaac tgtcgtcccg gagcagttga ctccggaacc ggaaagtgta gattccgaca
     3961 tccccgatgt ttgaggggtt ctgccgaagt ggtgaaactg gtagacacgc taccttgagg
     4021 gggtagtggg cgtacgcccg tggaggttcg agtcctctct tcggcaccat tcccgttcca
     4081 gctcggcccg gcaaaaatgt cgtttaaaac cgcgatgttg cgtggtgcgg cccgatattt
     4141 accggttgcg gggggcatga gccatcaata taatgcgcca cttgctgatg cggggtggag
     4201 cagtctggta gctcgtcggg ctcataaccc gaaggtcgtg ggttcaaatc ctgcccccgc
     4261 taccaaagga taaaagcccc tcctgtgctt tgcacgaaaa ggtcaggggc tttttattac
     4321 gccggggggc gcgtcttccc ggcgtaggcg cagcgggcct tatgaacccg ccatgacatc
     4381 cttgtaggtg gcccgccgcg taccggctgg gccgcccgtg gtctgatgcg aaagaagcga
     4441 tctcgcgcaa ttgcatcggg tatgagaatg ggcgtaagcc cattttttat tggctgaagg
     4501 aaagcctgac ggcgtttgac aagctgttga atgcgaagga gaaacagatc gaagcgttgc
     4561 tgacgcccac ggtagaaggg ctgggctgcc agatctgggg ggtcgagtac gcctcccagg
     4621 gtcggcattc gcgtctgcgt gtctacatcg accgcgagga cggcgtttcg gttgacgact
     4681 gcgagcgtgt gagccgacat gtcagcgacg ttctggatgt ggaagaggtg atatccggcg
     4741 gctatacgct ggaggtgtcc tctccgggca tggatcgggt cctgttcaag gcggagcagt
     4801 acgcggcgaa cgtcggccag cacgtggatg tccgtctcgt atatccgttc gaggggcgca
     4861 aacggatcgt aggggtgttg gcaggcctgg aggaagacga ttcctgcgga cacgtaatcg
     4921 tgcaggtgga tgatgaacag tacctgttgc cgctggagag cgttcagcgc accaggatta
     4981 ttccccagta tgactgaggt gcgaccaaag ttatgagcaa agagatgttg ttggtggtgg
     5041 ataccgtctc caacgagaag ggcgtcccga aggacgtgct tttcggcgcc gtggaggcag
     5101 cgctcgcgac tgccaccaag aagcgctacg acgacgacgc cgagttccgc gtcgccatcg
     5161 accgggagtc tggagagtac gagacgttca gagtctggac cgtggcggat ccggaggcgc
     5221 tggaagaaga ggaagagtgg aaccccgcgg ctcagctgac cgtggaggaa gcccgggaac
     5281 gggatgcgaa tcttcagctc ggcgatgtga tcgaggagcc ggtggagtcg gtgggctttg
     5341 gccgtatcgc tgcgcagacg gccaagcagg tcatcgtgca gaaggtccgt gaggccgagc
     5401 gtgctcaggt cgtcgaagct tacctgccgc gggtcgggga gctcgttaac ggaaccgtga
     5461 agaaactcgg gcgtgacagc gtcatcgtag atctcggcaa caacgccgag ggcctcctga
     5521 cccgggaaca cctgattccc cgggaaacgt ttcgtgtggg tgaccggttg cgcgcgcttc
     5581 tgctcgaggt caatccggac aatcgcggac ctcagctcat gctgacgcgc acctcgccaa
     5641 agatgctcat cgagcttttc aagctggagg ttcccgagat cgccgaggac gtcatcgaga
     5701 tacggggcgc ggcgcgggat ccggggtcca gggcgaagat cgcggtgaag accaacgacg
     5761 gtcggatcga tccggtaggc gcctgcgtcg gcatgcgggg ctcccgcgtg caggccgtat
     5821 ccggtgagct ggccggggag cggatagaca tcgttctttg ggacgacaac ccggcccagc
     5881 ttaccgtcaa cgccatggcg ccggccgagg tggccgccat cgtgctcgat gaggacaccc
     5941 acgccatgga tatcgcggtc accgaggaca acctcgctca ggcgatcggc cgcggcgggc
     6001 agaatgtgcg tctggccagt gatctggtcg gctggtcgct gaacatcatg acggaagagg
     6061 atgcggcggc gaaacaggaa gaggaggccg tgcgctacat tgaggagttc atgcgcgccc
     6121 tcgaggtcga cgaggacgtc gccaccgtgc tggtggaaga aggtttcacc tccatcgacg
     6181 aggtcgccta cgtgcccatc gaggagatga cggccattga agggttcgac gaggagatcg
     6241 tcgaggagct gcgcaaccgg gccaaggatg ctctgctgac gaaggcgatc gccagtgagg
     6301 agctgctcgg cgaaaaggag cctgcggacg atctgctgaa catggaaggc atggagcgac
     6361 gcctggcgtt ccagctggcc agcaatgaca tcatcacgat ggaagatctt gctgaacttt
     6421 cggtgcccga tttcatcgag atcgtcccgg aggtcgacga agagaccgcg gctgctctca
     6481 tcatgacggc ccgcgcgccc tggttcgaag aagcggaggc ggaagggtaa gccgccccga
     6541 agaggaaaaa gagtcatgtc ggaagtcaca gtcaagcaac tcgcagatac ggtgggggcg
     6601 cccgtagacc gtctgctcaa gcagatgcag gatgccgggc ttccacacga cagcgacgac
     6661 gcggcggtca cggaagagga gaagcagacg ctgctcgcct acctcaagcg cagccacgga
     6721 tctgacgacg ccgcgccaaa gaagatcacg ctgaagcgaa agaccgtcgg aaccctcaag
     6781 tccggccagg gcaggacggg ccgtaccgtg aacatcgagg tgcgccgtaa gcgcacctac
     6841 gtgaagcggg acgaggccga gaccgcgggc gcggcggcag aagcgccggc aaagccggcc
     6901 agccagtctg agctcgaggc gcagcgtatt cgggagcagg aactcgctcg caaagaggcc
     6961 gaagaggaaa cccggcgcaa ggaagcggag cgcaaggccg aagaggaaaa gcgggtagag
     7021 gagatcaagc gtcaggagga agccgacctt caggcgcgcg aaaccaaacc cggcgaggct
     7081 ccggcagaag actcagcggc gaaagcggcc gctgccgctg cagaggccca ggcgaaggcc
     7141 caggcaagcg agcgcggcgg gaaacgcgta cgcggcaagg agcgcgagcg gccgggcgaa
     7201 aaacgcgacg agttcggcga cggcaaaggc cgccgccggg agctctcgct caaaacgcag
     7261 cggcgtggca aacgtcgtca gcccgcaccg atgcccgtcg agcagcaggg cggggaattc
     7321 cgaccgacgg acttcatcgc gcgggaggtc gagatacccg acatcatcac ggtgggcgag
     7381 ctggcccagc gcatgacggt gaaggcaggc gaagtcataa aagcgctcat gggtatgggc
     7441 gtgatggcga cgatcaacca gagcgtcgat caggataccg ccacgctcat cgtcgaggag
     7501 ctgggccaca agccgaaacc catccacgag gatgccatgg agcaggctct ggaagcctcg
     7561 ctgcagatcg agggcgatgc cgcgaagcgg gcacccgtgg tgacggtcat ggggcacgtc
     7621 gaccatggca agacctcgct gctggactac atccgcaaga ctcgggtcac cagcggcgaa
     7681 gctggtggga tcacccagca catcggcgcc tacagcgtgg acacgccgca cggcaagatc
     7741 agcttcatcg atacgcccgg tcatgccgcc tttaccgcca tgcgggctcg tggcgccagg
     7801 gttacggaca tcgtcatcct ggtcgtggct gcggacgacg gcgtcatgcc ccagaccgag
     7861 gaagcaatac agcacgccaa agcggccggc gtgcctgtgg tcgtggccat caacaagatg
     7921 gacctcgaag gcgccgaccc ggaccgggtc acgaacgagc tggcggccaa ggaagtggtt
     7981 cccgaggatt ggggcggcga cactcagttc gtcaaggtct cggcggtctc cggcgaaggc
     8041 attgacgagc ttctcgaggc gttgctcctg caggcggaaa tcctggagct gacggccgtg
     8101 gaggatgctc ccgggcgcgg cgtcgtcatc gaatcgcggc tcgatcgcgg tcgggggccg
     8161 gtgtccaccg tcctggtgca gaacggcacc ctcaagcagg gggacgtgat cattgcgggc
     8221 gaatgccacg gccgcgttcg tgccatgctc aatgacaagg gtgagcaggt gaaagcggcg
     8281 gggccgtcgg ttcccgtcga ggtgctcggc atcaacggga ccccggcagc cggtgatgat
     8341 ttctcggttg caaccgacga gcgcaccgcg cgggagctcg ccgagttccg gcgggacaag
     8401 agccaggaac agcgtgttgc gacgcagcac gctgccaagc tcgaaaacat gtttgccaac
     8461 gtgggagccg gagagaagcg cgttctcaag atggtcctga aagccgacgt gcgcggcagc
     8521 ctcgaagcca ttacccaggc cctcaccgaa atgggtaacg aggaggtggc tgtcaacgtt
     8581 ctgggatccg gtgtcggtgg catcaccgaa accgacgcga acatggcgct cacctacggc
     8641 gcggcggtct tcggattcaa tgttcgagca gacaactctg ctaaggccat catcgagcgg
     8701 gaagggctgg atctgcgcta ctacagcgtg atctacgagc ttctcgacga cgtcaagcag
     8761 gtgctttccg gcatgctctc gccggaggtg cgcgaggaga tcgtggggat cgccgaggtt
     8821 cgggacgtgt tccgttcacc gcggtttggt caggtcgccg gctgcatggt caccgaaggt
     8881 acggtgtatc gcaacaaacc cattcgcgtc ctgcgcgaca acgtcgtgat ctacgaaggg
     8941 gagctggaat cgctgcggcg cttcaaggat gacgtcaacg aggttcgcag cggtacggaa
     9001 tgtggcatcg gcgttcgtaa ctacaacgat gttcgcgttg gtgatctcat cgaggtcttc
     9061 gatacgaaag aagtggcgcg ggagctctag gatgcgccgg gtaattgagc ctgtgtgggc
     9121 cgggatgcat gcgtgagttt ggacgtgatc tgcgggttgc cgatttcgtg cgcgacgagc
     9181 tcgctaagat catccagcgt gagatgcggg acccccgcgt gggcatggtc agcgtgaacg
     9241 aagtcactgt cagcagagac ctggcgttcg cggacgttta tgtgtcctcc atagaagcac
     9301 ggaccgccaa ggaacgcgaa gagctgatcg gcgtcctcaa caaggcagca ggtttttttc
     9361 gctcggagct ggccaagcgt cacagtatgc ggacgacgcc gaagccccga ttccactacg
     9421 acgagatggt cgaaagaggt ccggagctgg aagcccttat cgacttcgcc gtgggttccg
     9481 atacgcggga cgaggatcct ggtacggaag acgagctcgg tgaaggggag gggcggggcc
     9541 gtgggtagac ggggtcgcgg cagggacgtc agtggcgtgc tcgtggtcga caaacctgcg
     9601 ggatggagct ccaacgacgc cgtgcagaag gcaaagcgcc tgttcaacgc gcgcaaggtc
     9661 gggcacacgg gcgctctcga cccgcttgcc accggcgtgc tgccgctctg ttttggcgag
     9721 gctacgaagt tctcccagta cctgctctcc tcggacaaga agtacgaggt tcgaatcaaa
     9781 ctgggcgttg ccacggatag cggcgacgcc gacgggaacg tggtggcgga gcgggaagtc
     9841 gacgacgtca cgcccgcccg ggtggaggag gcgctgcgat tcttccgcgg ggagatcgat
     9901 caggttccgt cgatgttctc cgccataaag caccaagggc aacctcttta caagctggcc
     9961 cggcagggga tagaggtcga gcggcaggcg cgtcgcgtga ccgtctactc caacgagctc
    10021 atcagcctgg aaggcgacgt tgtcgagctg cacatccact gcagcaaggg cacgtacgta
    10081 cgaaccattg cccaggaact cggagaggtg ctcggatgcg gggcccacgt cagcgcgctg
    10141 cgtcgcacca tggccggccc ctacggcgag tcggacctgg tgaccttcgc ggagctggag
    10201 tcgtcgctcg aggcggaggg gaccctcgat cggttcctgc agccgatctc gagcaccgtt
    10261 cgggagtggc ccgaggttcg gctgacggac aacacggcct attatctgcg acaggggcag
    10321 cccgtgatgg tgccccacgc cccggccaga ggctgggtcc gtctggcaga gatcgttggt
    10381 gaggactcga cccgctttct gggggtcggg gaagtattgg acgatggccg ggtggcgcca
    10441 cgccgcctgg tcgttggtta gttgagttga ctgtaaatat gcacggtcat cttgaatgca
    10501 aacatgcata ttgatggaga aaccaaatgg cactaagcgc tgaaagcaaa gctgacatca
    10561 tcaaggagta tcagatcgag gagggggata ccggatctcc cgaggtgcag gttgcactgc
    10621 tcacacacaa catcaattcg cttcaggagc attttcgctc ccacaacaag gaccatcatt
    10681 cccgaagggg tctgatccgg atggtcagcc aacgccgcaa gctcctcgat tatctcagga
    10741 gcaaagacgc gtcgcgttac tcggagctca tcaagcggct cggcctgaga cggtaatccg
    10801 gacaacccgg tacggggcca aagattgata caggtagaaa tagagaagga aacggagaaa
    10861 tcattttgaa tccagttacg aaagaatttc agtttggtga tcagacggtg gtcctggaga
    10921 ccggccgcat cgccaagcag gccaccggcg cagtgctggt gacgatggga gatacggttg
    10981 tcctgtgcac cgtggtcggt gcgaaatccg cgcggccagg gcaggacttc ttccccctca
    11041 cggtgaacta ccaggagaag acctacgccg cgggcaagat tcccggcggc ttcttccgca
    11101 gggaaggtcg gccaagcgag aaagaaacgc tcacttgccg cctgattgac cggccgatcc
    11161 gaccgctctt cccgaagggg ttcctcaacg aggtgcaggt gatctgcacg gtcatgtcga
    11221 ccgacaagga tcaggatccc gacatcgctg ccttgctcgg aacgtcggcc gccctggcca
    11281 tatcgggcct acccttcaac ggaccgatcg cggcagcccg cgtcggttac ctcgatggca
    11341 gatacctcat aaatcctggc tacgctgcgc tcgagtcctc gctcctgagc atggtagtgg
    11401 cgggcacgga atcagccgtg ctcatggttg aatccgaagc caaggaactc accgaggacg
    11461 agatgctcgg aggcgttctc tacgcgcatc aggagatgca ggtagcgatc gaagcgatca
    11521 aggagctggc cgcaacggcc gggaaaccca agtgggattg gtcgccggcc gagtcgaacg
    11581 aaagcctcgt agccgaggtt tccgacaagg ttcgcgccga tctgggcaat gcctaccgga
    11641 tcaccgacaa gatggagcga caggctcggg tttccgagct gcgtgcggcg ctggtaggag
    11701 acctcggtgg tggcaacgag ccgagctttg cttcggacaa cgtcgccggc gcgttcggga
    11761 aggtggaaaa ggacatcgtg cgtcagcgcg tgctcgatgg cgagccgcgc atcgacggcc
    11821 gggatcttcg caccgtgcgt ccgatctcgg tcgaggtcgg ctttctgccc aagacccacg
    11881 gctcggcact cttcaccagg ggcgaaaccc aggcgatcgt cgtggcgacg ctaggcacca
    11941 tgcgcgacgc ggccctgatc gatgccctcg aagggaccta taaagacaac ttcatgctgc
    12001 actacaactt tccgccctac agcgtcggcg aagcgggctt catgacgggg ccgaagcggc
    12061 gcgagattgg tcacgggcgt ctggcccgtc gcggtgtgca ggccatgctg ccggacgtgg
    12121 acgacttccc ctacacgata cgggtcgtct cagagatcac cgagtctaac ggctcgagct
    12181 ccatggccag cgtctgcggg acctccctgg cgctgatgga tgccggggtg ccgatgaagg
    12241 cgccggttgc gggcgtggcg atggggctgg tgaaggaagg taacaagtac gccgtgctca
    12301 ccgacattct tggtgacgag gatcatctcg gagacatgga tttcaaggtg gctggatccg
    12361 ccaatggcat ctgcgcgctg cagatggaca tcaagatcga cggcatcacc gaggagatca
    12421 tggaaattgc cctggaccag gcgcgcgaag cacgcctgca catcctgggc gagatgaaca
    12481 aggtcctgga caggtctcga gagacgcttt ccgagaacgc gccgagcatg gtggtcctga
    12541 aggtgcatcc ggacaagatc cgcgacatca tcggcaaagg cggtgcgacc attcgaggca
    12601 tcgtcgacga aaccggtgcc gacatcgaca tcgaggacga cggctcggtg cgcatttacg
    12661 ccgaggacgc ggcgtcgaaa gacgcggcgg ttgcgcggat cgaggagatc accgcagagg
    12721 cggagatcgg caaggtctac accggcaagg tggcccgcat cgtcgatttc ggcgcgttcg
    12781 tgaacatcct gcctggcaag gatgggctgc tccacatctc tcagattgcc gaagagcgtg
    12841 tggagaacgt caccgattac ctccaggaag gccaggatgt ccaggtagtc gttctagaca
    12901 tcgatcagcg cggccgcatc aaactttcca tgaaggaagt ggccaactac gcgcaaagcg
    12961 cctgatcccc acccgctaag gccaggctga gccggtactc ggcgcagcct ggtctcagcg
    13021 gttggcccgg ttgtgtacca gatcgtccac gacgccaggg tccgccaggg tggacgtgtc
    13081 tcccagattc tccagatcgt cggcggcaat cttgcgaagg atgcgccgca tgatcttgcc
    13141 cgaccgggtc ttcggcaggc cgggcgcgta ctgaatgaac tccggccggg cgaagggtcc
    13201 gatctcggag cgcaccagcg cctccagctc ccctctgagc tgatccgggt cctcgttgtt
    13261 gccaaccatc agcgtcacgt aggcgtagat gccctctccc ttgacgggat gcggaaagcc
    13321 gaccacggct gcctctgcca ccgcttcgtg cagaaccagc gcgctttcga cctcggcggt
    13381 gccgatcctg tgccccgaga cgttgatgac gtcgtccacc cggccggtga tccagtagta
    13441 gccgtcggcg tcgcggcgcg cgccgtcgcc ggtgaagtag tagcccggat actgcgagta
    13501 gtaggtgtcg atgaccctct ggtggtttcc gtacaccgac cggatctggc ccggccacgt
    13561 attcgtaatc accaggttgc cgcttgcctc ctgttcctcg atgagccggc cgttttcgtc
    13621 catgagcccc agctccaccc cgaagaaggg ccgtgtggct gaaccgggtt tcagcgcggt
    13681 ggccccgggc aggggagaga tcatgatccc gccggtttcc gtctgccacc aggtgtcgac
    13741 gatggggcag cgctcctcgc ccaccacctt gtgataccac tgccacgcct ccggattgat
    13801 gggttcgccg accgtgccga ggagcttcag cgacgcccgc gacgtctgag tcacgtactc
    13861 gtcgccctgg cccatcagct gccggagcgc cgttggggcg gtgtagaaga tattgacctt
    13921 gtgcttgtcc accacctgcc agcagcgtga ggcgtcggga taggtcggca caccctcgaa
    13981 catgatggtc gtggcgccgt tggccagagg accgtagacg atgtaggaat gtccggttat
    14041 ccaacccaca tccgccgtgc accagtagat gtccccgtcg tggtagtcga agacgtactt
    14101 gtgggtcatg gccgcgaaga gaagatagcc ggcagtgccg tgctgtacgc ctttcggctt
    14161 gccggtggag ccggacgtgt agaggatgaa cgagggatct tcggaatcca tcacttcggg
    14221 ctcagccgtc ctgggctgat cggccaccag atcctcgtac cagatgtcgc gatccgggtt
    14281 ccaggggatc tcgttgcccg ttcgcttgac tgttacgacc gtgtggacgt tcggacaatc
    14341 cttcaaagcc gcctcggtgt tctccttcag cggcaccagc cggccgcccc ggacgccatg
    14401 gtctgccgtg atcacggtcc ggcagtcgga gtcgaggatc cggtctttca gagactgcgg
    14461 cgagaaaccg ccaaacacga cggagtgaat ggcgccgatg cgcatgcagg cgagcatggc
    14521 gtaggctgcc tccggcacca tcggcatgta gatgcagact cgatcgcctt tgcccacccc
    14581 gcgcgaccga agcccgtttg cgagctggca gaccgcatcg tgcagttcct gataggtgat
    14641 cgttttgtcg ttggcggggt cgtccccttc ccagatgatc gccgtctgac tcgcccggtc
    14701 gggcaggtgt cggtcgatgc agttgtacgc gatgttgagc ttgccgcccg cgaaccagct
    14761 gatgttgccg cgaaccatgt ccgcctcgcg tacctgatgc ggcttctcgt accaggcgac
    14821 gaacttgtct gcctgctccg accagaaacc ctcgggatcg gtgatcgacc gctcgtacat
    14881 cgcttcgtac tggtcgtcgt ttatgtagca gacgtcgagc acggactgcg gtacgggaac
    14941 gatgtgcgtt tcagacatgg cttcctccct tggcggggtt gttcggtgcg ggggtcatcc
    15001 cgtcgtcccg cgcagcagct cgaccagacc gcgcgtggag gcatcctggg tcatcgcgga
    15061 gttacctccg agctgctgga agatgggcac cgacagcgtc ttgccgagct ccacgccgaa
    15121 ctggtcgaac gagtttatgt tccagatcac gccctggcaa aaaaccttgt gctcgtacag
    15181 ggctaacaag gatcctaagc taaaagggtt cagctggcgc atgacgatgg tggtggtggc
    15241 atggtcgccc gctacgcgcc ggtgagcgtc gtccgcgtcc tggcccgcca tcatcgcctg
    15301 ggattgggcc aggccgttcg ccagcagcca tctgccgtgg acgtcgtcgg ggtccggctg
    15361 cccgaccatg atgaaatcgg ccgtgaacga gcgggtgccc tggtgcagga gctgatggaa
    15421 agcgtgctgg ccgttggtgc cctcgccgcc ccagatgatg ggagccgtgt gcaccgtcac
    15481 cgcttctccc tccagatcca cgctcttgcc gttgctctcc atttccagct gttgcaggta
    15541 gctgggtagc agaccaagcc tcgcgctgta cggaagaatg gcgtggttgc tcactccgag
    15601 gaagttgtag ttccagacac ctgccagggc catcaggacc ggcacgttgc cggacaaggg
    15661 cgagctacgg aagtgttcgt ccatcgcgtg cgcgccggcc agcagctcac gaaacgcgtc
    15721 cgcccccgcc gagatggcta cgggcaggcc gacggcggac cagagcgagt agcggccgcc
    15781 cacccagtcc cacatgggaa agatctgctc cgggcccatg ccgaacgccc gggcggcttc
    15841 gacgttctcg gtgatcgcca cgaagtgatg cttcaaggcg tctaggtcgg ccatgcgctc
    15901 gaggaaccat gcccgggcag aatcggcgtt gacgcgggtt tccagcgtgg tgaacgattt
    15961 cgacgcgacg atgaaaaggg tcgtctcggg atcgagcccg gtcagcgccg tgtcgagggc
    16021 ggatctgtcg atgtttgcaa cgaaacgcac actgggaccc gcgcccttcg ctccttccag
    16081 ggcccgaacg gctagctccg ggcccaggtg cgagccgccg atgccgatgt gcacgacgct
    16141 cttgatggcc ttgccggtgg caccccgcca gacgccggag cggacccgct ccgcgaagtc
    16201 gagcatgcgg gtcagggtcg tctcgacggc ctccccatag gcggcgggcc ggctcgccag
    16261 cggtgcgcgc agggcggtgt gcaaagccgg ccgctgctct gtgggattga cctgtgcgcc
    16321 ttcgaacagg tcgtctatcc ggcgatccag ccgggcggcc ccggccagcg ccagcagggc
    16381 atcgacgatc tcttcgttga gccgctgttt ggacaagtcg acgagcagtt ctccgacctc
    16441 gaagctcagc cgggcgaatc tctccggcga ccgcaaccag tcgctcaacg gttgtttgat
    16501 gctgtctgcc gaaagccggg tcaggctctt ccacggcgcg aggtcgtcgg gcctcatggg
    16561 ctggcccccg aagccctggt gccgcccggc aagatcggcc tgctcgcggc aggcgggccg
    16621 gggatgctgc gggcctgtgc ccctttagcg ctggcggcgg cggtgaatcc caacggtgcg
    16681 ttcaaagcag ggtgggattc gggttgaagt actgacgaag gccgtccatc gcgagtatct
    16741 ggtcgagcag ggactcgaag tgaacggcgt tgttggcgaa gtctatggac gctgcgttga
    16801 cgaccagcag gggcgcggcg tcatagaaat gaaagaactc cgtataggcc cgggccaggc
    16861 tttccaggta cccggcctcg atgtcgcgtt cgtagtcgat gccgcggtca cgaatgcgcg
    16921 ttcgcagaac gtccaccggg gcctgcaggt agatgacgag gtcgggcttt ggcgggtcga
    16981 tgtccagcgt ctcgtggatc tggcggtaga gggcgtactc gtcctcatcc agggtcatcc
    17041 gggcgaacag gtcgtccttc tccatgagga aatcggcgac cagggtgggc ccgagcaggt
    17101 cagcgtggtc gaggttggca atctgctgcg ccctgtggag caggaagaac agctgggtgg
    17161 gcaacgcgtt gcgccggccg tctcggtaga agctgttgag aaacgggttc tgcgcggcgg
    17221 gctccaggag aatcgggtag tgaaaggcct cggccagctt gttggcaagg gtggtcttgc
    17281 cgaccccgat ggggccctcg atgacgatgt aacgcggtag agattttcct tccagtttct
    17341 gcccgagcgc ggccgtagtt tcgatggcta gagagtgctg ggccggtggc gtcatggagc
    17401 ggatcagctg gtcggatgcc ttaataatgg acaaggcatc ccttcgcttc aaccacggcg
    17461 gcgccttctc ctccgctttc ttggtggctg gaccgccagt cgcgcctcgg tcatatcggc
    17521 ccgcgtggcc tggtctgcct gctggaaggt agtccaccac tgcgcagcat catcgagcgg
    17581 ttcgctgtcg gtggcctgct cgcccgactc cgcccgcagc acgagaaagt cgtaggcggc
    17641 ccggaatctg gggtgctgca cgatccggtc gatctgcctc ggtagcctgg cctccagacg
    17701 tggctgcagg ctccagacgt cgcgtacgaa ctgagagaac cggcgcggca tggacgtaac
    17761 cgcctgttga gcggccaggg tctcggacgc cgcctggtgc cgggcctcgg cgggctttcg
    17821 ctcgtcggtc agagcgttgc atcgggcctg gaagtcgtgc cacatgagca ccgcgagcag
    17881 aaagcccggg gtgacgggca gatcgctggc gatacgctgg tcggtattgg ccatggccag
    17941 ctcgaccagc cgggaatcgg gaggtgtgcc ggggaaaagc gcggcccgca gcggcgtgcc
    18001 cgcaatggct ttccaggcgg ctcccgcagc tccggacatc atcagcttgg
//
LOCUS       DBNHLM010000226        14104 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1589179, whole genome shotgun sequence.
ACCESSION   DBNHLM010000226 DBNHLM010000000
VERSION     DBNHLM010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 14104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14104
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1589179"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="AC16QF_10480"
     CDS             <1..608
                     /locus_tag="AC16QF_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449024.1"
                     /translation="QPGVEVKPIITLGGAHSVNTVFLTDVKVPVENRIGEEGKGWTYA
                     KGLLQHERTGLAGISRSIVALENLKAQAATVIRGNGTLMDDPGFKGKVAELEVDLMAV
                     EFTELRSLASASAGAAPGPESSILKLKGTEIQQRIQKLTVAAGGIYSAAWGARPVGHG
                     FAKGGMAGYLASRAYTIYGGASEVQKDVIAKNVLGIKRSGR"
     gene            605..1729
                     /locus_tag="AC16QF_10485"
     CDS             605..1729
                     /locus_tag="AC16QF_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449025.1"
                     /translation="MNFELSSEQQLLRDSVERFVDENYALETRRELAGSDTGFSSDYW
                     ATMAELGWLALPFAESDGGFAGTQIETMLLMEQFGRGLVLEPYFASIVLGGGAVRRGG
                     SAALKAEILPGVMDGSRQLTLAYAEEQARFDLDDVTTTARAEGDELILNGHKSMVANA
                     ATASALVVVTRTSGGQKDQDGVTLVLVDADADGVKIESFPTVDGLRAAEVTLTDVRAP
                     AANVIGGIGDGFAILNAVANDGILALAAEAVGAMEILYKDTVAYTQEREQFDHPLSDF
                     QVLQHRMVDMFVEYEQCKSLLYRATMEVASGAESAQRTVHALKHLIGKAGIAVGENAV
                     QTHGGMGMTEELRVGHYFKRLLVIDAQFGNADYHLQRFAA"
     gene            1765..2565
                     /locus_tag="AC16QF_10490"
     CDS             1765..2565
                     /locus_tag="AC16QF_10490"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6449026.1"
                     /translation="MQGRVALVTGGARGIGRGIAQAFLQAGARVMIGDLGGAGADWKY
                     ELSAESELKRTADELSAMGETHCVAVDVTDAASCARAVDATVQAFGRLDVLVNNAGVV
                     ASGDTESFPEADWDRVFAVNAKGIFLMTRAALPALRRSADAAIINTASIAGKRGFPKM
                     AAYCGSKFAAIGITQSLAAELAPEGIRVNALCPGMVGTAMWLEHLLPANAAKPDEKDD
                     QFKEAMARTIPMGRPQTPQDMGHAAVYLATAANVTGVSLSVAGGYEMN"
     gene            2562..3371
                     /locus_tag="AC16QF_10495"
     CDS             2562..3371
                     /locus_tag="AC16QF_10495"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR6449027.1"
                     /translation="MKKPTAVQNLFREPLLYFLLAGAAIFALAELTDGPGDDYLIEVS
                     DAERARISDQWQAQMGRAPTDEELAGLIEQWLREEIYYREALRMGLDRNDIIIRRRLA
                     QKLTFLTEDVIAGSTPSTEELRSYFEANAERYRIPERITFRHRYFSAERRPNAREDAA
                     AALTDEAVRGDPFMLQKAYALRSSREIADLFGVEFAEGLAQVAPGNWQGPIRSAYGWH
                     VVLVEQRAPGRLPSLEEVQKRAATDLSQERRSRANDEYYHSLRQRYEIIGG"
     gene            3368..4333
                     /locus_tag="AC16QF_10500"
     CDS             3368..4333
                     /locus_tag="AC16QF_10500"
                     /inference="COORDINATES: protein motif:HMM:NF025167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HIR6449028.1"
                     /translation="MKALAALLVVLAASQAKAHEVRPAYLQLTETAPGQFEVLWKQPL
                     LGDRRLPLEPVLPEACAREGPPIPEVAGAALIERWRTDCDLRSGAVTISGLSKTLTDV
                     LVEIRYLSAEPVSQLLKPSRPSLDLASPTPQIWSYLTFGVEHLLLGLDHILFVVGLVL
                     FIGNTWTLVKTITGFTIAHSITLACSVLGLISLPQAPVEAVIALSILFLARELVEPPE
                     RRSRITLGRPWLMAFAFGLLHGFGFAGALTEIGLPADQLAVSLLLFNLGIEIGQLLVI
                     GLLLSAAAIFRKAWRRNREVWPRRFAWVMGSVAGFWTIDRVLLVF"
     gene            complement(4366..4773)
                     /locus_tag="AC16QF_10505"
     CDS             complement(4366..4773)
                     /locus_tag="AC16QF_10505"
                     /inference="COORDINATES: protein motif:HMM:NF016060.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR6449029.1"
                     /translation="MTDFRTQSRRQVFRYLQVGFAGFVTDVAVLSALVYGMSLGETET
                     GLVGSRFVSFLAAVTVTFLLNARYTFGASIRDSRFPVYVLVQSLGAAITLGTYTALVL
                     HTTLSQAPVVALIIGSAAATTSNYVLVRRFVYV"
     gene            4897..5382
                     /locus_tag="AC16QF_10510"
     CDS             4897..5382
                     /locus_tag="AC16QF_10510"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR6449030.1"
                     /translation="MTTAPIECPAQTVEDGWIDYNGHMNMAFYNLAFDRALDHVYDRL
                     GIGADYVRLRGGSCFTREVHVNYLRELSAGDPIRVTFQLLDWDAKRLHFFEEMYHADQ
                     GYLAATSEQLALHVSMTSRSTHPFPQDIQAHLEELMTAHRPLPTPAQVGRVMGIRRSP
                     R"
     gene            complement(5398..7071)
                     /locus_tag="AC16QF_10515"
     CDS             complement(5398..7071)
                     /locus_tag="AC16QF_10515"
                     /inference="COORDINATES: protein motif:HMM:NF027688.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcineurin-like phosphoesterase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6449031.1"
                     /translation="MRLCCCVALALLGLGWSSFGLGSGTANGKVFEDLDGDGVWEPGE
                     PGVKGVRVSNGIAVVLTGEDGGYTLPVPEEAVIFITKPSGYATPVNAHQLPQFYYVHQ
                     PDGSPDHLRYPGVDPTGPLPERVDFPLRRQDEPARFQAILFADTQPQTEEELDFIRDD
                     VIAELIGTGARFGMTMGDILFDELSLFPRYNALIGTLGIPWYNVPGNHEMNFLAADDR
                     HALETFKRYFGPPYYAFEYADALFVVLDNIEYLGNGESDPADVRGTGGYVARIGRRQL
                     DWLAAELRHVPVDKLVFLGMHAPLATYLDDSPRNNTADRRELFGLLKGRPNLYAVAGH
                     THTTEHHYFGRDEGFAGPGELHHHVLSTVSGAWWSGPFDTRGVPTSDQRDGTPNGYHV
                     LEVDGTKVTVRYKAASAPADYQMRIMFDVVHHGLRKDAMRDFRHGQLFDGRFSVDQVP
                     AADILVNLFDGGPRSEVAFRIGDGEYLPMRRVRRADPYLIELHARNAETKKSWFKAEP
                     SSHIFVADVPDDLGPGVYPVGVRAIDEFGRAHHAHAVLEITGGSSPSGL"
     gene            complement(7084..8229)
                     /locus_tag="AC16QF_10520"
     CDS             complement(7084..8229)
                     /locus_tag="AC16QF_10520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449032.1"
                     /translation="MPMILNDEQNMLKDSAKDFCSNSAPISQLRRLRDENNADGFDRG
                     TWQAMVDLGWAGIPWPEEHGGLDFGYKGLGVVTEETGRTLAASPLFASVWVCGTLVNL
                     GGSEEQKGELLPLISTGELLMSLALEESHRHDPYGLETRAAAADGGFVLNGRKTFVLD
                     GHVADKLVVAARTSGERGDRNGITLFVVDSDAEGLSVTRTVMVDSRNAANVELDGVRV
                     GADAVLGAVGGAAEVLDPALDIARIGLCAEMLGSLQECFERTVEYLKEREQFGVPIGS
                     FQALKHRAANMFCEVELAKSCVLEALTALDENRDPQEIARLASLAKAKVGETFHLVSR
                     EGIQMHGGIGMTDEFDIGFFIKRAAVTEQTFGDVGFHRNRYGELEGY"
     gene            complement(8283..8816)
                     /locus_tag="AC16QF_10525"
     CDS             complement(8283..8816)
                     /locus_tag="AC16QF_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449033.1"
                     /translation="MQSRLCIACNWLVMLGALAIADLAAAEAAEAAGCDAAEYRAFDF
                     WVGDWEVRLADGSLAGHNAVRHEEGGCMVRESWRGASGGTGSSINYFEPVNRIWRQIW
                     VSPTTNIDISGTAAQGSVVLSGTITYRASRERFPFRGSWTRLPDGRVRQYFEESRETG
                     VWQPWFEGFYTRQQPSD"
     gene            9106..9792
                     /locus_tag="AC16QF_10530"
     CDS             9106..9792
                     /locus_tag="AC16QF_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR6449034.1"
                     /translation="MTNTGPATARPTDMGDAQQRLNATLPMARLEPVTLPDCSGMRLA
                     LINADFPTGPLPGEVMSAVVERPAYWAFCWGSGLGLAQFLLRNPEWVAGKSVVDLGSG
                     SGVAGIAAARAGAARVVACDTDADARLATSVNAAMNGVRLEVAATLPARCDLMLMADV
                     LYDRANLPLLSTAQRHADEVLVADSRIRELPDPEYVQVTTIDAATFPNLGEFDEFGTV
                     RVFHRPSARV"
     gene            complement(9789..10526)
                     /locus_tag="AC16QF_10535"
     CDS             complement(9789..10526)
                     /locus_tag="AC16QF_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449035.1"
                     /translation="MSRSLSWFLAFLVLLGVLAVAGSWYLGPSLVGLAFDADRREAPY
                     YVLSLAGRSELADADLRFGSDLAELFDAAGGKLLWRGTTERVVKGRLQDEWLNAQLFA
                     FPRGANFVELKTGGDYRDLLAAYEGRSRLLLGSPTQPDRMARGRIWVLNLIAEDAEDS
                     QATGGDPLAGLMGGVADFGGSLVWDAKVADLDERSSWNRLVLLGFAQPLEAEVWLSDA
                     GTQTELALSMERARRRVTLLMRPADPI"
     gene            10669..11502
                     /locus_tag="AC16QF_10540"
     CDS             10669..11502
                     /locus_tag="AC16QF_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6449036.1"
                     /translation="MYEEITYTVEDPVAIITMNRPDRLNAFTVRMLAEIRHALGAAEK
                     DGRVVGIVLTGAGRGFCAGMDMGSLDRMSSGGEAAAEDLSGLEAEVGDPGMGEDFQVT
                     YGYLMAVQKPVIAAVGGACAGLGFVIAMLTDMRFVEPQAKFTTAFSQRGLVAEHGVSW
                     ILPRLIGPGRALDILWSSRKFDGTEAERIGIAERLVDEGAAVSEACDYIRDLAANASP
                     TSLKMMKAQVYRHLNMSLGEAMRESNAWMAQSLKREDFTEGVRSFIERRPPRFKRVGE
                     D"
     gene            11534..12322
                     /locus_tag="AC16QF_10545"
     CDS             11534..12322
                     /locus_tag="AC16QF_10545"
                     /inference="COORDINATES: protein motif:HMM:NF014635.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HIR6449037.1"
                     /translation="MSDLITTDQTSYDWAELVAGLNRLLRLRTTPIGMKLFETRGEME
                     AVPRIRRPRDIHTTDQIVGQAARNGWTVGITAEDLVGAQCSTVIGLHPRSDDWLSGDR
                     MTGVWYETGEDASAHQHAMDVVPHGRYEAMAVSPLASGRLDPPDICLIYATPAQMILL
                     INGLQWSGYRKLEWGCVGESACADSWGRALATGEPSLSIPCFAERRYGGVMEDELLMA
                     MPPKFLPKTLAGLEQLSRNGLRYPIPQYGVNNDVRAGMGVSYGR"
     gene            complement(12338..13381)
                     /locus_tag="AC16QF_10550"
     CDS             complement(12338..13381)
                     /locus_tag="AC16QF_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449038.1"
                     /translation="MLQGRYSEAELYSEHDYARPHQIAGRRLHGGFDAAGNYVPPRSK
                     GRSEAIEAWTRALRERGGELFDADASLLSGPRMPTVEQQHMLLREGIGRPFWNTLTVT
                     GKIEGRGRVLAEMQFPDLQDVVVEDISDKAVGHLNRGLLAIHGIDEGGQPELGLGGHD
                     VMWFVARDLVFGPGAYPDVEPPESIARPEAGKRWMPEISAEHEALLSFLMNLLIIEFR
                     AEIGFAATQDILRADDLFTDRRTRACEAAEIIERIRADEEIHVCSLRLYLGELRQLTL
                     RTVDGGTVPAAELIDGFWDGLVRWATVEQPQLAAERQYAAIRTEILEHPDGERVLAAF
                     DALSDPAGLASAG"
     gene            complement(13453..>14104)
                     /locus_tag="AC16QF_10555"
     CDS             complement(13453..>14104)
                     /locus_tag="AC16QF_10555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449039.1"
                     /translation="LVFARTDADKGRNGVTCFIVDTDTPGFHVRRIVHTLRTARYATE
                     LSFEDMRVAHGNVLGEVNGGFAIANDRLSRQRIPYAAGCIGVAIKAHEMALEYVPQRE
                     TFGAPLSTRQAIQWMLVDNEIDIRQASWLTLEAAHKADKGEPFRKEAAMAKLVATEAG
                     SRVVDRCMQMFGGYGVTKDFPFERWYREMRIRRIGEGPNEVQRHIIARDILGASLR"
BASE COUNT         2522 a         4785 c         4540 g         2257 t
ORIGIN      
        1 agcagcccgg cgtagaggtc aaacccatca tcaccctcgg aggtgcccac agcgtcaaca
       61 cggtgttcct caccgacgtg aaggttcccg tggaaaaccg catcggcgag gagggcaagg
      121 gctggaccta cgcaaaagga cttctccagc atgaacgcac cggccttgcc gggatttccc
      181 gctccatcgt ggctttggag aacctgaaag cccaggccgc aacggtgatc cgcggcaacg
      241 gaaccctaat ggacgacccc ggctttaagg gcaaggtcgc cgagctggag gtcgacctca
      301 tggcggtgga gttcaccgag ctgcgaagcc tcgcttcggc ctcggcgggc gcagcccccg
      361 ggcccgaatc ctccatactc aagctcaagg gcaccgagat acagcagcga atccagaagc
      421 tcacggtggc agccgggggc atctactccg ccgcctgggg ggcacgaccc gtcggccacg
      481 gtttcgccaa gggcggcatg gccggctacc tggccagccg tgcctacacc atttacggcg
      541 gggcgagcga ggtgcagaaa gacgtcatcg ccaagaacgt gctgggaatc aagaggtcgg
      601 gccgatgaac ttcgaacttt cttcggaaca gcagctgctg cgcgacagcg tggagcggtt
      661 tgtcgacgaa aactacgcgc tggagacccg ccgcgagctt gccggcagcg ataccggctt
      721 cagcagcgac tactgggcga ccatggccga gctcggctgg ctggccctgc ccttcgcgga
      781 atccgacggc ggcttcgccg ggacccagat cgagaccatg ctcctgatgg agcagttcgg
      841 ccgcggcctg gtcctggagc cctacttcgc cagcatcgtc ctcggcggcg gcgcggtgcg
      901 tcggggcggc agcgcggcgc tgaaggcgga gatcctgccg ggcgtcatgg acggcagtcg
      961 ccagctaact ctcgcctacg ccgaagagca ggcgcggttc gacctggacg acgtcaccac
     1021 cacggcccgg gccgaaggcg acgagctgat cctcaacggt cataaatcca tggtggcgaa
     1081 cgccgccacc gcctctgcgc tggtggtggt gacgcgcacc agcggtggcc agaaagacca
     1141 ggacggcgtc actctggtac tcgtggacgc ggatgccgac ggcgtgaaga tcgagagctt
     1201 tcccaccgtg gacggcctgc gggcggcgga ggtgacgctc accgacgtgc gcgcgcccgc
     1261 agccaacgtg atcggcggaa tcggagacgg tttcgccatc ctcaacgccg ttgccaacga
     1321 tgggatcctg gcactggcgg cggaggcggt cggcgccatg gaaatcctgt acaaggacac
     1381 cgtcgcctat acccaggagc gcgagcagtt cgaccatccg ctttccgact tccaggttct
     1441 tcaacaccgc atggtggaca tgttcgtgga gtacgagcag tgcaagtcgc tgctgtaccg
     1501 cgccaccatg gaggtggcct ccggcgccga atcggcgcag aggacggttc acgccctgaa
     1561 gcacctgatc gggaaggccg ggatcgccgt cggcgagaac gcggttcaga cccacggcgg
     1621 catgggcatg accgaggagc tgcgcgtcgg ccactacttc aagcggctgc tcgtcatcga
     1681 cgctcagttc ggcaacgccg actaccattt gcagcgcttc gcggcctaag tagaatccgg
     1741 cctccgcaca gggggaagca cgtcatgcag ggcagagtcg cgctggtaac cggcggcgcc
     1801 cgcggcatcg gccggggcat cgcacaggcc tttctgcagg ccggggctcg ggtgatgatc
     1861 ggcgacctcg gcggcgccgg ggccgactgg aagtacgagc tgtctgccga atcggagcta
     1921 aaacgcacgg cagacgagct gtcggccatg ggcgagaccc actgtgtggc cgtcgacgtg
     1981 accgacgccg ccagctgcgc gcgcgcggtg gacgccaccg tgcaagcctt cggaaggctc
     2041 gacgtcctcg tcaacaatgc cggcgtcgta gcctcgggag acacggaaag cttccctgaa
     2101 gccgactggg atcgcgtgtt cgcggtgaac gccaagggga tctttctcat gacccgggcc
     2161 gccctgccgg cgctgcggcg atcggccgac gcggccatca tcaatacggc ttccatcgcg
     2221 ggaaagcggg gctttccgaa gatggccgct tactgcggct ccaagttcgc cgccatagga
     2281 atcacccagt ccctggcggc cgagctcgcg cccgagggca tccgcgtcaa cgccctgtgt
     2341 cccgggatgg tggggaccgc catgtggctc gagcacctgc ttcctgccaa cgcggcgaag
     2401 ccggacgaaa aagacgacca gttcaaggag gcaatggcgc gcaccattcc catgggccgg
     2461 ccccagaccc ctcaagacat gggccacgca gccgtctacc tagccacggc cgccaacgtc
     2521 accggcgtca gcctgagcgt tgccggcggc tacgagatga attgaaaaag ccgacggccg
     2581 tgcagaacct tttccgcgaa ccgctgcttt acttcctgct ggcaggcgcg gccatcttcg
     2641 ccctcgccga gctcacagac ggccccggcg acgactacct gatcgaggtt tccgacgcgg
     2701 agagggcacg gatctcggat cagtggcagg cccagatggg ccgggcgccg accgacgagg
     2761 agcttgcggg cctgatagag cagtggttgc gcgaagagat ctactatcgg gaagcccttc
     2821 gaatggggct ggatcgcaac gacatcatca tccggcgccg tcttgcccag aagctcacgt
     2881 tcctgaccga ggacgtcatt gccggttcca ccccctccac cgaagagctt cgaagctact
     2941 tcgaagccaa cgcggagcgg taccgcatac ctgagcgaat cactttccgg caccgctact
     3001 tcagcgccga gcgccggcct aatgccagag aggatgccgc cgccgccctg acggacgagg
     3061 ccgtacgcgg ggatcccttc atgctccaga aagcctacgc gctgcgctcg agccgggaga
     3121 tcgccgatct tttcggcgtc gagttcgccg aagggctggc ccaggtggcg cccgggaact
     3181 ggcagggacc gatccgctcg gcctacggat ggcacgtggt gctcgtggag cagcgggctc
     3241 ccgggcgact gccgtcgctc gaggaagtgc agaagcgggc ggcgacggac ctgtcacagg
     3301 aacgccgaag ccgagccaac gacgagtact accactcgct gcgtcagcgg tacgagatca
     3361 tcggcggatg aaggcgctgg cggcgctgct cgtcgttctc gctgcgagcc aggctaaggc
     3421 ccacgaggtc cgcccggcct acctgcagct gacggaaacc gcaccgggcc agttcgaagt
     3481 gctctggaag cagccgttgc tcggcgaccg gcgcctgccc ctggaaccgg tgctgcccga
     3541 ggcgtgcgcg cgcgagggcc cgccgatccc ggaggtcgcc ggcgctgcgc tcatcgaacg
     3601 ctggcgcaca gactgcgacc ttcgaagcgg agccgtcacc atctccggcc tctccaagac
     3661 gctgaccgac gttctcgtgg agattcgcta cctgtcggca gagccggttt cccaattgct
     3721 caagcccagc aggccatcct tggatctggc gtcaccgacc ccgcagatct ggagctacct
     3781 cacgttcggc gtggagcacc tgctgctcgg gctcgaccac atcctgttcg tggtgggcct
     3841 cgtgctattc atcggcaaca cctggactct ggtgaaaacc atcactggct tcaccatcgc
     3901 ccacagcatc acgctggcct gctcggtgct ggggctgatc tcgttgcctc aggcccccgt
     3961 ggaagcggtg atcgcgctgt cgatcctctt tcttgcccgg gaactggtgg aacctcccga
     4021 gcggcgctcg cgcatcaccc tgggccgccc gtggctgatg gccttcgctt tcggtctgct
     4081 tcacggcttc gggtttgccg gcgcgctcac cgagatcggg ctgcccgcag atcagctggc
     4141 cgtctctctg ctgctgttca acctcggcat cgagatcggc caactcctcg ttatcggcct
     4201 gctccttagc gctgcggcga ttttcaggaa ggcctggagg cgcaaccgcg aggtctggcc
     4261 caggcggttc gcctgggtca tggggtccgt cgcggggttc tggaccatcg accgggtgct
     4321 gctcgtcttc tgagatcgat ctggctgccg ccgcgaaccc gatgctcaca cgtagacgaa
     4381 gcgtcgcacc agcacgtagt tgctggtggt cgctgccgcc gaaccgatga tcagggcgac
     4441 cacgggcgcc tgggacaagg tggtgtggag caccagcgcg gtgtaggtgc cgagggtgat
     4501 ggcagccccc agcgactgga ccagcacgta gacggggaac ctcgagtccc ggatcgaggc
     4561 gccgaaggtg tacctggcgt tgagaagaaa ggtcaccgtc accgcagcga ggaaagaaac
     4621 aaagcggctt cccacgagcc ccgtctcggt ctcaccgaga gacattccgt agaccagcgc
     4681 cgagagcacg gccacgtcgg tgacgaagcc cgcaaagccg acctgaaggt agcggaacac
     4741 ctgtcggcgg gactgcgtgc ggaaatctgt caattcacac ctcgtccagg caccttcacc
     4801 ccaagcttac gtggcagcca tgtgcccagc aaggcgaccg ccttagcctg ccggcgcttt
     4861 ggggttacca tcgggaaaga ctctggggag acgctcatga ccaccgcacc catcgaatgc
     4921 ccggcacaaa ccgtggagga tggctggatc gactacaacg gccacatgaa catggccttc
     4981 tacaacctcg ccttcgaccg ggccttggat cacgtctacg accggctggg catcggcgca
     5041 gactacgtac ggctgcgcgg cggctcctgc ttcaccaggg aagtccacgt gaactacctg
     5101 cgcgagctaa gcgccggcga cccgatccgg gtgacgtttc agctgctgga ctgggacgct
     5161 aagcggctgc actttttcga agagatgtac cacgcagacc agggctacct tgccgctacc
     5221 tcggagcagc tggcgcttca cgtgagcatg acaagccgat caacgcaccc ctttccccag
     5281 gacatccagg cgcatctcga ggaactcatg acggcacatc ggccgcttcc gacgccggct
     5341 caggtcggac gggtcatggg catcagacgc agcccccgct gaaacggcgc aggaaaatca
     5401 gaggcccgac ggcgagctgc cgccggtgat ttccagcacc gcatgcgcgt ggtgcgcgcg
     5461 accgaactcg tcgatcgcac gcacgcccac cgggtagacg cccggaccga gatcgtcggg
     5521 aacgtccgca acgaagatat gcgacgacgg ctcggccttg aaccaggact tcttcgtctc
     5581 ggcgttgcgc gcgtgcagct cgatcagata gggatccgcg cgcctcaccc ggcgcatcgg
     5641 caggtactcc ccgtcaccga tgcggaatgc cacctcggag cgcggacctc catcgaagag
     5701 gttgaccagg atgtctgcgg cggggacctg atccacggag aaacgaccat cgaacagctg
     5761 cccgtggcgg aagtcacgca tggcgtcctt gcgcagaccg tggtgcacca cgtcgaacat
     5821 gatgcgcatc tggtagtcgg cgggcgcgct ggcagccttg tagcgcacgg tcaccttcgt
     5881 gccgtccacc tccaggacat ggtacccgtt gggggttccg tcgcgctgat ccgacgtggg
     5941 cacgccgcgg gtatcgaacg gcccgctcca ccacgccccg gaaaccgtgc tgagcacgtg
     6001 atggtggagc tcaccgggcc cggcaaatcc ttcgtccctg ccgaagtagt gatgctcggt
     6061 ggtgtgcgtg tggccggcga cggcgtacag attgggccgg cctttgagca atccgaagag
     6121 ctcacgccgg tcggcggtat tgtttctcgg gctgtcatcg aggtaggtgg caagcggggc
     6181 gtgcatgccg aggaaaacca gcttgtcgac gggcacgtgg cgcagctctg ccgccagcca
     6241 gtcgagctgc cgcctgccga tcctggcaac gtagccgccg gttccccgga cgtcggccgg
     6301 atcgctttcg ccgttaccga gatactcgat gttgtccaga accacgaaga gcgcatccgc
     6361 atactcaaag gcgtagtaag gcggaccgaa ataccgcttg aacgtctcaa gcgcgtggcg
     6421 atcgtcagcc gccaggaaat tcatctcgtg attgcccggc acgttgtacc agggtatgcc
     6481 cagggtcccg atgagcgcgt tgtagcgcgg aaaaagcgag agctcgtcga acaggatgtc
     6541 acccatggtc ataccgaagc gcgcgccggt gccgatgagc tccgcgatca cgtcgtcccg
     6601 gatgaaatcc agctcctcct cggtctgggg ctgggtgtcg gcgaacagga tggcctggaa
     6661 acgggcgggt tcatcctgcc ggcgcagcgg gaaatcgaca cgctcgggca acgggccggt
     6721 gggatccacc ccggggtagc gcagatggtc gggcgagccg tccggctggt ggacgtagta
     6781 gaactgcggc agctggtgag cgttcaccgg cgttgcgtaa ccgctgggct tggtgatgaa
     6841 gatcacggct tcctcgggaa ccggcagcgt gtagccgccg tcctcgccgg tcagcaccac
     6901 ggcgatgccg ttggagaccc tcacaccctt cacaccaggc tcgccgggtt cccagacgcc
     6961 gtcgccgtca agatcctcga agacttttcc gttcgccgtt ccggagccaa gcccgaagct
     7021 cgaccagccc agaccgagca gggccagcgc gacgcagcag cacagcctca acgtcccggg
     7081 cccctagtaa ccttccagct ctccgtatcg gttccggtgg aagcccacgt caccgaaagt
     7141 ctgctcggtc accgccgctc gcttgatgaa aaacccgatg tcgaactcgt cggtcatccc
     7201 gatgccgcca tgcatctgta tgccttcccg ggaaaccagg tgaaacgtct cgcccacttt
     7261 cgctttcgcg aggctagcca gccgggcgat ttcctgggga tcgcggttct cgtcaagcgc
     7321 cgtcagggct tcgagcacac aggatttggc aagctcgacc tcgcagaaca tgtttgccgc
     7381 gcggtgcttg agggcctgaa aggaaccgat gggcacgccg aactgctcgc gctccttgag
     7441 atactcgacc gtgcgctcga agcactcctg gaggctgccc agcatctcgg cgcagaggcc
     7501 gatgcgggca atgtcgagcg ccgggtccag aacctcggcc gcgccgccga cggcgccgag
     7561 caccgcatcg gcgccgaccc gaacgccgtc cagctcgacg ttcgcagcgt tgcgcgagtc
     7621 caccatgacg gtgcgtgtca ccgacagccc ttcggcgtcg gaatccacga caaacagcgt
     7681 gatcccgttc cgatcccccc gctcacccga agtccgggcg gcgaccacga gcttgtcggc
     7741 cacgtgcccg tcgagcacga aggtcttgcg cccgttcagt acgaacccgc cgtctgcggc
     7801 ggcggccctg gtttcgagcc cgtaagggtc atgacggtga gactcctcga gcgcgaggct
     7861 catcagcagc tcaccggtgg agatcagcgg cagcagctcg cccttctgct cctcgctgcc
     7921 acccagattc acgagggtgc cacacaccca gacgctggca aaaagcgggc tggccgccag
     7981 ggtccgaccg gtttcttcgg tcaccacgcc caggcccttg tacccgaagt cgagcccccc
     8041 gtgctcctcc ggccagggaa tgcccgccca gccgagatcc accatggcct gccaggtgcc
     8101 ccggtcgaag ccgtctgcgt tgttctcatc gcgcagcctg cgcagctgcg aaatgggcgc
     8161 gctgttgctg cagaagtcct tcgcgctgtc cttgagcatg ttctgctcat cgttgaggat
     8221 catcggcatg atcgcccccg gaatcatcga gcggccaata caaccgcgtc gcaccgacgc
     8281 tgtcaatccg aaggctgctg acgggtgtaa aacccctcga accagggctg ccagacgccg
     8341 gtttcgcgcg actcctcaaa gtactgccgc accctgccgt cgggtagccg ggtccagctg
     8401 ccgcggaagg ggaagcgttc ccgggaggcg cggtaggtga tggtgcccga cagaacgacg
     8461 ctgccctgcg ccgccgtgcc ggagatgtcg atgttggtgg tcggcgacac ccagatctgg
     8521 cgccagatcc ggttcaccgg ctcgaagtag ttgatgctcg accccgtgcc gccgctggca
     8581 ccccgccagc tctcgcgcac catgcagccg ccttcctcgt ggcggacagc gttgtgcccg
     8641 gccagcgatc cgtcggccag gcgcacctcc caatcgccga cccagaaatc gaacgcacgg
     8701 tactcggccg cgtcgcaccc ggccgcctcg gcagcctcgg cagccgcaag atcggcgatc
     8761 gccagcgcgc ccagcattac gagccagtta cacgcgatac aaagcctcga ctgcatggcg
     8821 ccccatctcc cgctaggata ctcggcataa tcagataaaa aggtggtgat aacaacggac
     8881 cgcgccccga gaaccatcgg tgacagcaac ccgagacagc gccctaccgc ctgcccggga
     8941 cgctcccacc gtaaccgagc gcatcctcag tcaacgcacc aggtatgagg tcaccatacc
     9001 caccgccggc gagcggcccc tcccccgctc gccggcaacc ctcgttcaaa agatttcctc
     9061 cccgctagcc tcgaaacccg gcccccgggc aaatatagtg cgcccatgac gaataccggc
     9121 ccggcaaccg ctcgaccgac agacatgggc gatgcgcagc agcggctgaa cgcgaccctg
     9181 cccatggccc gattggagcc ggtgaccctt ccggattgct cgggcatgag gctcgccctg
     9241 atcaatgcgg actttcccac gggaccccta ccgggagagg tgatgagcgc ggtggtcgaa
     9301 cgacccgcgt actgggcgtt ctgctggggc agcggtctgg gccttgctca gttcctgctg
     9361 cgcaatcccg agtgggtcgc cgggaaatcc gtcgtcgacc ttggctcggg atccggcgtg
     9421 gcggggattg ctgcggcgag ggccggggct gcacgagtcg ttgcctgcga tacggatgcc
     9481 gatgcgcggc tggccaccag cgtgaacgcg gcgatgaacg gcgtgcggct ggaagtcgcc
     9541 gccaccctcc cggcgcgatg cgacctgatg ctcatggccg acgttctcta cgaccgcgcg
     9601 aacctcccgc tgctctcgac ggctcagcgc cacgccgacg aggtgctggt ggcagattcc
     9661 cgaatacgcg agctgcccga tcctgagtac gtgcaggtca ccaccatcga cgcggccacg
     9721 tttccgaacc tcggagagtt cgacgagttc ggcacggtgc gggtatttca ccgcccttcg
     9781 gccagggtct agatcggatc ggccgggcgc atcagcaggg tgacccggcg ccgcgctcgc
     9841 tccatgctga gcgccagctc ggtttgggtg ccggcatcgg aaagccacac ttcggcctcc
     9901 agcggttgag cgaaaccgag cagcaccaga cgattccagg acgagcgctc gtccaggtcc
     9961 gcgaccttcg catcccagac cagggagccg ccgaagtccg cgacgccgcc catcaagcca
    10021 gccagggggt cgccgccggt tgcctgcgag tcctcggcat cctccgcgat caggttgagg
    10081 acccagatac ggcccctggc catccgatcc ggttgggtcg ggctgccgag caacagacgc
    10141 gacctgccct cgtaggccgc cagcaggtcg cggtagtcgc cgcccgtctt cagctccacg
    10201 aagtttgcgc cacgcgggaa ggcaaacagc tgggcgttca gccactcgtc ctgcagacgg
    10261 cccttgacga cccgctcggt ggtgccacgc catagcagct tgccaccggc cgcgtcgaac
    10321 agctcggcaa gatcggagcc gaatctcaga tccgcgtcgg ccagctcgct ccggcccgcc
    10381 aggctcagaa cgtagtaggg cgcttcccga cggtccgcgt cgaacgccag cccaaccagg
    10441 ctggggccca gataccagct tccagcaacg gccagaacgc cgagcagcac cagaaatgcc
    10501 aggaaccatg atagcgagcg gctcaccact gcacccgaga ctttccaaaa gccgacatga
    10561 taaaccatcg ttcccgctgt gcagcgccgc cgtcaaggcc cgccaggcat tactcggctc
    10621 gcgggatgcg gtacattcca tcggaaacgg cccgctccgg agcgccacat gtacgaagaa
    10681 atcacctaca cggtcgagga tccggtcgcg atcatcacca tgaaccgacc agaccgcctc
    10741 aacgctttca ccgtccggat gctcgccgag attcggcacg cgctgggagc ggcagagaag
    10801 gacggccgcg tggtcggcat cgtgctgacc ggggcgggcc gcggattctg cgccggcatg
    10861 gacatgggca gcctggaccg tatgtcctcg ggtggcgagg cggcggcgga agatctctcc
    10921 ggcctcgaag ccgaggtggg ggaccccggg atgggcgagg acttccaggt gacctacggc
    10981 tacctcatgg ccgtgcagaa gcccgtcatc gcggcggtgg gcggggcctg cgcggggctc
    11041 ggcttcgtca ttgccatgct caccgacatg cgcttcgtgg agccccaggc caagttcacg
    11101 accgcttttt cccagcgcgg cctcgtagcc gagcatgggg tgagctggat cctgccccgg
    11161 ctgatcgggc ccggccgggc cctggacata ctctggagct cgcgaaaatt cgacgggacg
    11221 gaagccgagc gtatcggcat cgccgaacgc ctggtcgacg agggcgccgc cgtcagcgag
    11281 gcctgcgact acatacgcga cctggccgcc aacgcctcgc cgacctccct gaaaatgatg
    11341 aaggctcagg tgtaccggca tctcaacatg tcgctgggcg aagccatgcg cgagagcaat
    11401 gcctggatgg cccagagcct caagcgcgag gacttcaccg agggcgtgcg ctcgttcatc
    11461 gagcgcaggc cgccacgatt caaacgcgtg ggcgaggact gactcccgca acgcgccaag
    11521 acgaggaccc cccatgagcg atctgataac gacggaccaa acctcatacg actgggccga
    11581 gctggtggcc ggtctgaacc ggctgttgcg gctgcgcact accccgatcg ggatgaagct
    11641 gttcgagacg cggggggaaa tggaggcagt gccgcgcatc cgacggccgc gggacattca
    11701 cacgacagat cagatcgtcg gccaggccgc ccgcaacggc tggacggtgg gcatcaccgc
    11761 cgaggacctc gtcggcgccc agtgcagcac ggtgatcggc ctgcatccgc gcagcgacga
    11821 ctggctctcc ggcgaccgca tgaccggagt ttggtacgag accggcgagg atgccagcgc
    11881 tcaccagcac gccatggacg tggtgccgca tggtcgctac gaggccatgg ccgtgagccc
    11941 gctggccagc ggcaggctcg atcctcccga catctgcctc atctacgcga cgccggccca
    12001 gatgatcctg ctcatcaacg ggctgcagtg gtcgggttac cgcaagctgg aatggggatg
    12061 cgtgggcgag tccgcctgcg cggactcgtg gggccgggcg ctggccaccg gcgagcccag
    12121 cctctccatc ccgtgctttg ccgagcgtcg ctacggcggc gtcatggaag acgagctgct
    12181 catggcgatg ccgccaaagt tcctgccgaa gacgctggcc gggctggagc agctctcccg
    12241 caacggcctt cgatacccca taccccaata cggcgtgaac aacgacgtgc gcgcgggcat
    12301 gggagtgagc tacgggcgct gacgcgtccg gccagaatca gccggccgac gccaggcccg
    12361 ccggatcgct gagggcgtcg aacgccgcca ggacccgctc gccgtccgga tgctccagga
    12421 tctcggtccg gatagcagcg tactgccgct ccgccgccag ctgcggctgc tccacggtgg
    12481 cccatcgaac caggccgtcc cagaagccgt cgatcagctc ggcggccggc accgtgccac
    12541 catctaccgt gcgcagcgtg agctggcgaa gctcgccgag ataaagccgc agcgagcaca
    12601 cgtgaatttc ctcgtccgcg cgaatgcgct cgatgatctc cgctgcctcg caagcccgcg
    12661 tgcgccggtc agtgaacagg tcgtctgccc gcaggatgtc ctgagttgcg gcaaacccga
    12721 tttccgcccg aaactcgatg atgagcaggt tcatcagaaa gctgagcagc gcctcatgct
    12781 cagcggatat ctcaggcatc caccgtttcc cggcctcggg ccgggcgatg ctttccggcg
    12841 gctccacgtc cgggtatgcc ccggggccga aaaccaggtc gcgggccacg aaccacatca
    12901 cgtcgtgccc gcccaggcca agctccggct gtccgccttc gtctatgcca tgaatggcga
    12961 gcagcccgcg gttgaggtga ccgacggctt tgtcggagat gtcttccacc acgacgtcct
    13021 gaagatcggg gaactgcatc tcggcgagca cccggccgcg cccctcgatc ttgccggtca
    13081 cggtcagggt gttccagaac ggccggccga tgccttcgcg gagcagcatg tgctgctgct
    13141 cgacggtggg cattcgcggg cccgaaagca ggctcgcatc ggcatcgaac agctctccgc
    13201 cgcgttcgcg cagcgcccgg gtccacgcct cgatggcctc cgagcgcccc ttggaacggg
    13261 gcggcacgta gttgccggcg gcgtcaaagc ccccgtgcag gcggcgcccg gcgatctggt
    13321 gtggccgcgc gtagtcgtgc tcgctgtaga gctcggcctc actgtagcgt ccctgtagca
    13381 tttgcagcct cgtagcgtcc caggagcgcc ggttataaca gattgcgccg gaagccgtcc
    13441 cggcaaagcg tttcagcgca gagacgcccc cagaatgtcc cgggcaatga tgtgtcgctg
    13501 cacctcgttg gggccctcgc cgattcgccg gatgcgcatt tcccgatacc agcgctcgaa
    13561 ggggaagtcc ttggtcaccc cgtagccgcc gaacatctgc atgcaccggt ccaccacccg
    13621 ggagcccgcc tcggtggcca ccagcttggc catggccgcc tccttacgga acggctcgcc
    13681 cttgtcggcc ttatgcgcgg cctccagggt gagccaggag gcctgccgta tgtcgatctc
    13741 gttgtccacc agcatccact gaatggcctg acgtgtggac agcggggcgc cgaacgtctc
    13801 gcgctgggga acgtattcca gggccatctc gtgagccttg atcgcgacgc cgatgcaacc
    13861 ggcggcatag ggtatgcgct gtctggagag ccgatcgttg gcgatggcga agcccccgtt
    13921 tacctctccg agcacgttgc cgtgggccac cctcatgtcc tcgaaggaga gctcggtggc
    13981 gtagcgggcc gtgcgcagcg tgtgaacgat gcgccgaacg tgaaagcccg gcgtgtccgt
    14041 atccacgatg aagcaggtga ccccgttgcg tcccttgtcg gcatccgtgc gcgcaaacac
    14101 gagg
//
LOCUS       DBNHLM010000227         3704 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1589238, whole genome shotgun sequence.
ACCESSION   DBNHLM010000227 DBNHLM010000000
VERSION     DBNHLM010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1589238"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="AC16QF_10560"
     CDS             <1..596
                     /locus_tag="AC16QF_10560"
                     /inference="COORDINATES: protein motif:HMM:NF019229.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HIR6449040.1"
                     /translation="SGPYVKQQSFQGRDGYMGGFADIDHDGDEDLIFAGDSVAYLNDG
                     GGAFRPGPSIPMTGISDPRAIAFGDIDDDGDLDFVVTDDDGESRLIRNDLAQGNWLKV
                     DLRSPQGQAGAFGARAYVYPPGGMPDEPRCESPVGEELIGMRESRGNHGYLAQDDPVL
                     HFGLGEHDPVDVVVCFLDGSETTVTAVPAGQTLQIRP"
     gene            894..1583
                     /locus_tag="AC16QF_10565"
     CDS             894..1583
                     /locus_tag="AC16QF_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449041.1"
                     /translation="MGHLQASTIRGFALALGTALLIGCSGSSKQTASEEPAPAETASS
                     TQEQSPPPAEAEPAPEEEAPAPAPVSVSLSAADDMVASGGSTTLSWTSSGADLCEATG
                     GWTGEKSVSGSENVGPIDASTTFTLSCSNATENGVAMVTVDLAGSLSVSWSAPTENVD
                     GTPLDDLNGYRVYYGDFSGSYVGSQDVADSLATTTNLSLAAGQYYVAMTAIDIDGNES
                     DFSNEVLKTAR"
     gene            complement(1633..2928)
                     /locus_tag="AC16QF_10570"
     CDS             complement(1633..2928)
                     /locus_tag="AC16QF_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449042.1"
                     /translation="MPNRAGPRWLWLLLAGTAAPAASAFEIGAAIGHESEYTSNTERS
                     ADDEVAEWIHSPRVEIGAQHEGASLNLQAGYVIERRYYQKDIYDDEKADRGSAEIRWQ
                     ALPSRLDFTLRNVRAETTRQSFAADTRDNRQVVAYTEAGPTLRLQPRRGDELQVEYLY
                     TTVNASETDTDSQRHTGSLRYVLGLSTNQALTVESSYRDIDYDNESSPDVSGWSHTGT
                     YAANTRLLELSLTGGFSRLDRTLGRESTSGRILRANLAWERGNSRLGFNVSEELRDQS
                     TNISRSDQDFGDTANEDSDLNEVFTETRAELSWEQEFGRSTFSAGLYASEEDYEDLAE
                     RDSERFGARFRVSRRISRQTRVSLRADFANREYSGRNEKYDELRASIALERSVGQSLS
                     LSLSAGYDESRGKGASGRSYDEWTTRIGLRYQALGDRRR"
     gene            complement(2939..3568)
                     /locus_tag="AC16QF_10575"
     CDS             complement(2939..3568)
                     /locus_tag="AC16QF_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system exopolysaccharide export
                     protein"
                     /protein_id="HIR6449043.1"
                     /translation="MNTLRVREFVKFAAIFLLTTAALGGCASDGAPPPASNTTIPDFA
                     EDYRIGVGDTLSVSVWRNDDLSITAPVRPDGKISVPLAGDVLVGDKTPEEVSAEITEK
                     IGAYIRDPFVTVAVTNMGSTEYRSRVRVTGAVDRPVSLPYRQGMTVLDVVLEAGGINE
                     FANGSKSTLFRRSGERFEINLDRLLKRGDMSTNFTLQPGDVITVPERAF"
BASE COUNT          616 a         1172 c         1230 g          686 t
ORIGIN      
        1 cgtcagggcc gtacgtcaag cagcaatcat ttcagggccg cgatggctac atggggggct
       61 tcgccgacat cgaccatgac ggcgacgagg atctgatctt tgcgggcgac agcgttgcct
      121 accttaacga cggtggtggc gcgttccgcc ctggaccgag catccccatg accggcatat
      181 ccgacccgcg cgccattgcc ttcggcgata tcgacgacga cggtgatctc gacttcgtcg
      241 tcaccgacga cgacggagag agccgtctga tcaggaacga cctggctcaa ggcaactggc
      301 tcaaggtcga tcttcgatcc ccccagggtc aggcgggtgc cttcggggcc cgcgcctatg
      361 tttatccgcc tggcgggatg ccggatgagc ctcgctgtga aagcccagtg ggtgaggagc
      421 tgatcggcat gcgggagtcg cgcggcaacc acggctacct ggcccaggac gatccggtgc
      481 tccacttcgg tctcggcgag catgatccgg tggatgtggt tgtctgcttt cttgatggtt
      541 ccgagaccac ggtgacagcc gtccctgccg gccagactct tcagatcagg ccctgatacc
      601 gcccccctcg cggcaggctc accgagtcga gccgagctgc tggcggctgg tgattccggc
      661 ctgcagaacg tatgtgatcc tttcgctcag ggcgtcggga cacgcatccc aaatccttcg
      721 actcccgccg gtgatgttga tcagttcgca ctccgcgatg gtcgaattgt ctaaattccg
      781 ctcacgcttc ctcggttacc catcgagacc ggctggagag gctcgggtcg aacaggtgcg
      841 gaaggaccct cccagggagc gatgcagttc gagggaaaga ggatttaggg accatgggac
      901 acttgcaggc ttccaccatc aggggtttcg cattggcgct gggcacggcg ctgctcatcg
      961 gctgctcggg gtcgtccaag cagacggcat ccgaggagcc cgcgccggcc gagaccgcgt
     1021 cgtcgaccca ggagcagagc ccgccgcctg ccgaggcgga gccggcgccc gaagaggaag
     1081 cccctgctcc ggctcctgtg tccgtcagcc tgagcgcggc ggacgacatg gtggccagcg
     1141 gcggctcgac gacgctgagc tggacgtcgt ccggcgcgga tctctgcgaa gctacgggcg
     1201 gctggacggg cgaaaaatcg gtgagcggct ccgagaacgt ggggccaatc gacgcgtcca
     1261 cgacgttcac gctgtcctgc tccaacgcca ccgagaacgg ggtcgccatg gtcacggtgg
     1321 atctggccgg ctcgctcagc gtcagctggt cggcgcccac ggagaacgtc gacggcacgc
     1381 cgctcgacga tctgaacggg taccgcgtgt actacgggga tttctcgggc agctacgtcg
     1441 gctctcagga cgtggcggat tcgcttgcga ccaccacgaa ccttagcctg gcggcaggac
     1501 agtactacgt ggcgatgacg gccatcgaca tcgatggcaa cgagagcgac ttctccaacg
     1561 aggtgctcaa gacggcccga tgagttggcc ggggaactgc gcggtcgccg ctaccgccgt
     1621 gggcccggtc gatcagcgcc gccgatcgcc caacgcctgg tagcgcagcc cgatgcgcgt
     1681 cgtccactcg tcgtagctcc ggccggaggc acctttaccg cggctctcat cgtagccggc
     1741 gctcaacgaa aggctcagcg attgaccaac gctgcgctcg agggcgatgc tggcgcgcag
     1801 ctcgtcgtat ttctcgttgc gaccggagta ctcgcgattg gcgaagtccg cccgcaggct
     1861 caccctcgtc tgccgagata tgcgacgtga aacgcggaac ctggccccga agcgctcgct
     1921 gtcgcgctcg gccaggtcct cgtagtcttc ctcactggca tacaggccgg cggagaaagt
     1981 cgaccggccg aattcctgct cccaggacag ctctgcacgg gtttccgtga acacctcgtt
     2041 caggtcggag tcctcgttgg ccgtatcgcc gaaatcctgg tcggaacggc tgatattggt
     2101 ggattggtca cgcagctcct cgctcacgtt gaagcccagc cgcgagttgc cgcgttccca
     2161 ggcgaggttc gcgcgcagga ttcggccgct ggtgctttcc cggccgaggg tgcggtcgag
     2221 acggctaaag ccgccggtaa gggagagctc cagcaggcgt gtgtttgcgg cgtatgtgcc
     2281 ggtatgggac cagccgctga cgtccggcga ggattcgtta tcgtagtcga tatcccggta
     2341 actcgactcg acggtgagcg cctggttcgt cgatagcccc agcacgtagc gcaatgagcc
     2401 ggtatgacgc tggctgtccg tgtccgtctc ggaagcgttg acggtcgtgt acaggtactc
     2461 gacctgcagc tcgtcaccgc ggcgcggctg gaggcgcagg gtgggtcccg cctcggtgta
     2521 ggcgaccacc tgacggttgt cccgcgtatc cgctgcaaag ctttgccgcg tggtctcggc
     2581 acgcacgttg cgcagggtga agtcgagccg gctcggcagc gcctgccagc gaatctccgc
     2641 gctgccccgg tcggctttct cgtcgtcgta gatgtctttc tggtaatagc ggcgctctat
     2701 gacgtagccg gcctgcaggt tgagggaggc gccttcgtgc tgcgcgccga tctcgacccg
     2761 gggactgtga atccactcgg cgacctcgtc gtccgcggag cgctcagtat tcgaggtgta
     2821 ctcggattcg tggccgatgg cggcgccgat ctcgaaggcg ctcgcagcgg gtgctgcggt
     2881 gccggcgagc agcagccaca gccaacgggg tccggctcgg ttgggcatcg agatctgcct
     2941 agaaagcccg ctcgggcacg gtgatgacgt caccgggttg cagggtgaag ttcgtgctca
     3001 tgtcgccgcg cttgagcagc cggtccagat tgatctcgaa gcgctcgccg ctgcgccgga
     3061 acagggtcga tttcgatccg ttggcaaact cgttaatccc accggcctcg agcaccacgt
     3121 cgagaaccgt catgccctgg cggtaaggca gcgacacggg ccggtccacc gctcctgtca
     3181 ccctcacccg ggagcgatac tccgtgctgc ccatgttcgt gacggcgacg gtgacgaaag
     3241 ggtcgcgaat gtacgcgccg atcttctcgg taatctccgc gctgacctcc tccggcgtct
     3301 tgtcgcccac gagcacatcg cccgccagcg gtaccgaaat ctttccgtcc ggccgcacgg
     3361 gtgccgtgat gctgagatcg tcgttgcgcc agacgctcac cgaaagcgtg tcgcccacgc
     3421 cgatccggta gtcctcggca aagtccggga tcgtcgtgtt cgaggcgggc ggcggcgcgc
     3481 cgtcggatgc gcagccgccg agggcggcgg ttgtcaggag gaagattgct gcaaatttga
     3541 cgaattcgcg aacgcggagc gtattcattc tgggtcacct gagtcggggt tacagacggt
     3601 gggatcatac cccttaatgg ggtcgttggg gcgtgccgtt accagcccat tacataggct
     3661 cacatgagcg caacagacgt cgatgggtag ttcgtcaaca atga
//
LOCUS       DBNHLM010000228         5913 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_750473, whole genome shotgun sequence.
ACCESSION   DBNHLM010000228 DBNHLM010000000
VERSION     DBNHLM010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_750473"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="AC16QF_10580"
     CDS             <1..394
                     /locus_tag="AC16QF_10580"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6449044.1"
                     /translation="FHYTLFPNFAVSIWADGFHFLRARPHPTDPERCVFDNWWYASCP
                     EKDLRPVPTGMGLIDRDADVRTEVFDYGEGWVGAGIDQDVEVFVKQQRGFRSRGFKGV
                     YLSRQESRVRRYHELIDDYIEGRLPNAR"
     gene            493..2052
                     /locus_tag="AC16QF_10585"
     CDS             493..2052
                     /locus_tag="AC16QF_10585"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6449045.1"
                     /translation="MFKAWNQADAPGLVAGVFRDGERLWMGSFGLSNLEFDAPNTPQT
                     RFNAGSVAKQFTATAILRLEQSGELSLDDPIRRYVPELPGVARSITLRQMLHHTSGLR
                     DVWALTDLGGWQPADVRTPTQALRILARQRALNFLPGNGFGYSNSGYLLLAEVIRRTT
                     GRPFPDWMRQHVLEPAGMEDTYFYEDPIKLLPGVASPYRFLGRSRGFARDDLNSGLAG
                     SGNLVTTAADLGRWSAHLLTSKIGGAPLVERLSEQGILPDGRKTGYGMGLFVGTHRGL
                     PVVSHGGANAGYRSELLMFPDQRVAVVVLGNVNTVSAERLARSMADVVLAEDLPAPSP
                     LPETTPVPMALPPASFAGRYALGQMLLEIRESDGSLYFRIGGGDLRPLTATGPVTFAT
                     SEPGVSLEFQLDENRSITGVDLLAAGRTLPAERLEPEMLTATDARAYEGRYHSEELDT
                     SYRVVYENGHLVARHLRGSDIVLTAFGQDRFLESAGGDLRVDFQRSRRGRITGFEVSV
                     DRARAIAFDRI"
     gene            complement(2101..3471)
                     /locus_tag="AC16QF_10590"
     CDS             complement(2101..3471)
                     /locus_tag="AC16QF_10590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spinster family MFS transporter"
                     /protein_id="HIR6449046.1"
                     /translation="MGGPSAGSGAALQRPGTAAAWYATVILTLAYTFSFVDRQVLNLL
                     VEPIRHDLGLSDTSISFLQGLAFVIPYVLMSVPIGRLVDKFNRILVLIAGVLFWSVAT
                     FGCGVAGNYGQLMAARMGVGVGEASLTPAAWSLLADYFPPDKLARPVSFFLMGPYLGA
                     GLALIGGAAVIDWAASIGELSLPVLGSIRAWQFTFMMVALPGVLIAALLAMLPNPQRQ
                     REAGDAEPAPGWSEVMRFMKRHARIYLAVLLGVPFLVVVLYGLQGWVPTILVRVYGWD
                     LASAGRIYGTLALVAGSAGVLSGPVVGGWLKRRGYADYPLRLATGAAAAVALSMVTLP
                     FQTSGAGALVCIGLASFFVTLPLALMTFVTQTVTPRNMRGVLAGFFVVGTNVIGLGLG
                     PTLVAATTDYVLGDPGKVHVSLMIISVCVAPVAMLLLWSGMRALGEWERQNQGSPDVE
                     DSTAGR"
     gene            complement(3505..3855)
                     /locus_tag="AC16QF_10595"
     CDS             complement(3505..3855)
                     /locus_tag="AC16QF_10595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6449047.1"
                     /translation="MIIVVARLVFDTQEHRDKAVELSTDIQGRTRDEEPGCRMYCFAP
                     DPCVPAEIQVYELWDDAASLEAHFKHPNYAAMVEALGSAGGLLESINRQYVADDRGTV
                     YGDDRTPRIQALLD"
     gene            3992..4708
                     /locus_tag="AC16QF_10600"
     CDS             3992..4708
                     /locus_tag="AC16QF_10600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6449048.1"
                     /translation="MRTQEGSDARDLGELSDIEAVSRLLDVKDLRLIDVGCGAGHLAR
                     SLAELGAIVLGIEPDPAQAERNRTADPVPNVTLLEAGAEKIPADDHSVDGVMFFRSLH
                     HVPRQLMDASLIEAARVLKPEGFLCVVEPGMEGSHYAMMRPFRDETEVRTLAQEALAR
                     TAEGLFDSAHGYLYTQHPRHDSFEAMVEFFTSLSFNDISREMIDVPEVHKHFEAARTD
                     EGYVFEQPTLVNVYRHPKAN"
BASE COUNT         1150 a         1938 c         1797 g         1028 t
ORIGIN      
        1 gtttcactac acgctgtttc cgaacttcgc ggtgtcgatc tgggccgacg gctttcactt
       61 tctgcgggcg cggccccatc ccaccgatcc ggaacggtgc gtcttcgaca actggtggta
      121 cgccagctgc cccgagaagg atctgcgtcc cgtgccaacc ggaatgggcc tgatcgaccg
      181 tgacgcggac gtgcgtaccg aagtattcga ctatggcgaa ggctgggttg gagccggcat
      241 agatcaggac gtggaggttt tcgtcaagca gcagcgcggc ttccggtccc ggggtttcaa
      301 gggcgtctac ctgtccaggc aggagagccg ggtgcgccgc tatcacgagc tcatcgacga
      361 ctacatcgag gggcgcctgc ccaacgcgag gtaagttcga cgaggacaaa cgctattgcg
      421 ggactgactg ctctctctgc agccatttgc ctgtcagggg cagccgtcgc tgccacctcg
      481 gacctgaacc cgatcttcaa ggcctggaac caagcggatg ctcccggcct ggtcgcgggc
      541 gtgttccgag acggcgaacg gctgtggatg ggaagcttcg gcctgtcgaa cctggagttc
      601 gacgcgccca acaccccgca aacccgtttc aacgcggggt ccgttgccaa gcagttcacc
      661 gccacggcca tcctcaggct cgagcaatct ggggagctca gcctggatga cccgatacgt
      721 cgatacgtgc ccgagctgcc tggggttgcc aggtccatta ccctgcggca aatgcttcat
      781 cacaccagcg gtctgcggga cgtctgggcg ctcacggact taggcggctg gcagccagcc
      841 gacgtgagga cgccgaccca ggctctgcgt atcctggcgc gccagcgggc gctgaatttt
      901 ttgcccggca atgggttcgg ctactccaac agcggctatc tcctcctggc cgaagtgatc
      961 cgacgtacca ccggcaggcc gtttccagac tggatgcgcc agcacgtctt ggaaccggcc
     1021 ggcatggagg atacctactt ctacgaggac ccgatcaagc tgctgccagg cgtcgccagc
     1081 ccgtaccgct tcctcggcag aagcaggggt tttgctcgtg acgatctcaa cagcgggctg
     1141 gcaggcagcg gcaatctcgt cacgacggca gccgacctcg ggcgatggtc ggcgcatctc
     1201 ctgacatcga aaatcggggg cgctccgctc gtcgaacggc tctccgagca gggcatcttg
     1261 ccggacggtc ggaagaccgg gtacggcatg ggcctgttcg tcgggaccca ccgcggcctt
     1321 cccgtggtct cgcatggagg cgccaacgct ggataccgaa gtgagttgct gatgttcccc
     1381 gatcagcggg ttgccgtcgt ggttctgggc aacgtcaaca cggtctcggc ggagcggctg
     1441 gcgcgatcca tggcggacgt cgtcctcgca gaagacctgc ccgccccttc cccgcttccg
     1501 gaaaccacac ccgtgcccat ggccctgccg cccgcaagct ttgccggccg ctacgccctg
     1561 ggccagatgc tgctggagat ccgcgagtcc gacgggtccc tttatttccg cattggcggc
     1621 ggcgacctgc gcccgctgac cgccaccgga ccggtgactt tcgccacctc ggaaccgggc
     1681 gtctcgctgg aatttcagct tgacgagaac agatcaatta ccggcgtcga tctgctcgct
     1741 gccggacgca cgctcccggc cgagcgcctc gagccggaaa tgcttaccgc cacagacgct
     1801 cgggcttacg agggccgcta ccacagcgag gaactcgaca cctcataccg tgtcgtttac
     1861 gagaacggcc acttggtcgc cagacacctg cgcgggagcg atatcgtgct cacggccttt
     1921 ggtcaggacc gtttcctgga atcagcaggg ggtgatctga gggtcgactt tcagcgctcc
     1981 cggcgcggtc gaatcaccgg cttcgaagtg tccgtcgatc gggcccgggc catcgccttc
     2041 gaccggatct gaatccccag gagtgatcag acgacggccc ggaaaccgac ccagccaccc
     2101 tcatctccca gccgtcgaat cttccacgtc gggcgatccc tgattctgcc gctcccactc
     2161 cccaagcgcc cgcatgccgc tccacaggag caacatggca acgggggcca cgcagaccga
     2221 gatgatcatc agcgatacgt gcactttgcc gggatcgccg agaacatagt ccgttgtcgc
     2281 cgcgacaagc gtcgggccaa gccccagccc gatgacgttc gtcccgacga cgaagaagcc
     2341 cgccaggacg ccacgcatgt tgcggggggt cacggtctgc gtgacgaagg tcatcagcgc
     2401 cagcggcagc gtgacgaaaa agctggcaag cccgatgcag acaagcgcgc ccgcgcccga
     2461 cgtctggaac ggcagggtca ccatgctcag cgcgacggcc gccgccgcgc cggtggccag
     2521 acgcagcgga tagtcggcat atccccgccg cttcagccag ccgcccacga cgggaccgct
     2581 gagcacgccc gccgagcccg ccaccagcgc cagggttccg tagattcgtc cggcgctggc
     2641 gaggtcccag ccgtatacgc gaacgaggat cgtcggtacc caaccctgca gcccgtagag
     2701 gacgaccacg agaaagggga cacccagcag cacggcaagg tagatacggg cgtggcgttt
     2761 catgaatcgc atgacttcgc tccagcccgg tgccggctcg gcatcgccgg cttcccgctg
     2821 acgctgggga ttggggagca tggccagcag cgccgcgatc agaacgcccg gcaacgccac
     2881 catcatgaag gtgaactgcc acgcgcggat gcttccgagc acgggcaacg acagctcacc
     2941 aatggaggcc gcccagtcga tcacggcggc tccaccgatc agcgcaaggc ccgcgcccag
     3001 atacggtccc atgagaaaga agctcacggg acgcgccagc ttgtccggag gaaagtagtc
     3061 ggcaaggagg gaccaggccg ccggggtcag agaagcctcc cctaccccca cacccattct
     3121 cgcggccatt agctgtccgt agtttcccgc cactccgcag ccgaaggtcg ccacgctcca
     3181 gaacagcacg cccgctatca gcaccaggat gcggttgaac ttgtccacca ggcgcccgat
     3241 gggcacgctc atcagcacgt aggggatgac gaaggcgagc ccctgcagaa agctgatgct
     3301 ggtgtcgctg agccccagat cgtgcctgat cggctcgacc aggaggttga gaacctgccg
     3361 gtcaacgaaa gagaacgtgt aggccagcgt caggatgacg gtcgcgtacc acgcggccgc
     3421 cgtccccggt cgctgcagag cggcgccgga gcctgccgaa ggcccaccca ccgctatgga
     3481 atcctctgcg ttaccgcggg cacctcaatc cagcagagcc tggatacgcg gcgtgcgatc
     3541 atcgccgtag acggtgccgc gatcgtctgc tacgtattgg cggttgatgg actcgagaag
     3601 acccccagcg ctgcccaggg cttccaccat ggccgcgtaa ttagggtgct tgaagtgcgc
     3661 ttccagcgac gcggcatcat cccaaagctc gtacacctgt atctcggccg gcacgcaggg
     3721 atccggcgcg aagcagtaca tgcggcatcc aggctcctcg tcccgggtcc gaccctggat
     3781 gtcggtggac agctccaccg ccttgtcgcg gtgttcctga gtgtcgaaaa cgagccgggc
     3841 aactacgatg atcatgtctt tctcctgatc ttatgtggca ccgaagagca gccaagaacg
     3901 ggcgattccg tgcggcgcca tccgcagcaa cgtctaccgc ggatgaaacg gcgtgaggcc
     3961 ctcccttcgc cctcattaaa ccggcgcgaa attgagaact caagaaggtt ccgacgcccg
     4021 cgatctcggt gagctatcgg acatcgaagc cgtatcacga ttgctggacg tcaaagatct
     4081 ccgtctgatc gacgtcgggt gcggcgccgg ccacctcgct cgctcccttg ccgagctggg
     4141 cgccatcgtc ctgggcatcg agcccgatcc ggcacaggcg gagcgtaatc ggaccgcgga
     4201 tcctgtcccg aacgtgacgc tgctcgaggc gggggccgag aagatccccg cggacgacca
     4261 cagcgtcgac ggcgtcatgt tcttccgctc gctgcaccac gtgccgcgcc agctgatgga
     4321 cgcgtcgctc atcgaggcgg ccagggtgct caagcccgaa ggattcctct gcgtcgtgga
     4381 gcccggcatg gaggggagtc actacgcgat gatgcggccg ttccgcgacg aaacggaagt
     4441 gcgcaccctc gcccaggagg ccctggcccg aacggctgag ggcctgttcg atagcgccca
     4501 cggctacctc tacacccagc acccgcgcca cgacagtttc gaggccatgg tggagttctt
     4561 caccagccta tcgttcaacg acattagccg ggaaatgatc gacgtccccg aagtgcacaa
     4621 gcatttcgag gcggcgcgta cagacgaagg ctatgtcttc gagcagccca cgctggtgaa
     4681 cgtctaccgc cacccgaagg ccaactgacc ggccaatcgg cccgtccgag cgttcttttt
     4741 catcgggaat cggacattct gaggactcgt tcccattggt cggcccgcca tatgaggcga
     4801 tgttctcatc ctggagccac ttcgctggat acactcgatg caaccgtcaa aggggtcgag
     4861 catgaaaaca ttcatggtcc tatccgcagt tctgttgcta tcgacctgcg tggcgggcga
     4921 gcaacatcgc gatgacgtgc ccatcatggc cggcgtcgtt gatacgcgct cgggtgacat
     4981 ccgaaggtgc gagtggaaag gctacgacgt ccggcccgac tgcctgcctg cacaaacctc
     5041 cccgccctga tcgtctccca cccgcctgac ccgttcggtt tagcagctac cttcgccacg
     5101 cgacgacgat cactccacaa tttaccgact accgaaaccc gtttcctcca acccatccaa
     5161 agttcgacat cgcgcgcgcg gggtggttga ttcaaatcca tcgggtgcga gccgagcgaa
     5221 ccgagagcgg atcggactct ctgtgcgttg tcctcgatac cgacaggcct agaaacaggc
     5281 tgattcacac gctccttgca ccggctacca cccttttgat ggaatactca cccacaagtg
     5341 acgtgcctgc atacctgcac gaagttgcca acctcatcgg gtagcaatgg aggagccgcc
     5401 gcattagcag gtcgttccac cgaaaagagt cagcaagaac gctggagagc agggcttcgg
     5461 aaccaaattt ggctggagat tcaagatgaa gcgacgtggt ccactggcgc tgattgttgc
     5521 gggcgtcctg gcaaacaact atatcctttt gcacgacgtt atctgggaca aatattcgga
     5581 ctactggatt gaggggtcgc accaagttat cgccctgggc ccacgaagtt gggcgggtat
     5641 tatcttttgt cttatcgtga ttgctgccgg aatctggtgg gcaaacaggg tgatcgggca
     5701 aaagtcaggc ggatgacccc tcgaagtcac caagtccgtc gggggtgcac atggaacagc
     5761 cgctcgacta aaccataccg cgcagacggc agggtcgaag ggagttatca atatcgtagc
     5821 gccaacgatt tcagtgaggt gcgccgatga ttccaagtac cgacaacgtt ctggacttaa
     5881 ctggcaccta tctccgtgaa gatcggaaga gca
//
LOCUS       DBNHLM010000229         2912 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_93149, whole genome shotgun sequence.
ACCESSION   DBNHLM010000229 DBNHLM010000000
VERSION     DBNHLM010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_93149"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="AC16QF_10605"
     CDS             <1..310
                     /locus_tag="AC16QF_10605"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6449049.1"
                     /translation="VLVNSCLTLSLVTGMSVSDVSQKTVANLGWNNVRLSGPVFVGDT
                     IYAESEVLEIRESKSRPNQGIVTIRSRGIKQDGTEVISFERAMLIPARGHAVDDAIDY
                     "
     gene            321..1469
                     /locus_tag="AC16QF_10610"
     CDS             321..1469
                     /locus_tag="AC16QF_10610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449050.1"
                     /translation="MAELSEQDRLVLDSVDRFLDRDVRPVARALEQEDAYPHELVEKM
                     KALGLFGATIDERHGGLGLTASVYALIVERVSAVWMSVSGIFNSHLIMCAAVQRFGTE
                     AMKERLLPAMATGELRGGIALTEPDCGTDLQAIRCRARRDGESYVIDGTKQWITNAVE
                     GNVLAVLVKTDPDAVPAHRGMSLLIVEKSAGYKATKLKKLGYRGVDTGEVQFDGVRVP
                     AANLVGDGEGRGLQQILSGLALGRINVAARGVGVADASLQAALRYAQERQTFGVPIHD
                     HQAIQIKLADMATRVEAARLLTEKAARIYDTGQRCDLEAGMAKLFASEAALSNSLEAM
                     RIHGAYGYSPDNDVERYYRDAPLMAIGEGTNELQRIIIARQLIERNPI"
     gene            complement(1495..1680)
                     /locus_tag="AC16QF_10615"
     CDS             complement(1495..1680)
                     /locus_tag="AC16QF_10615"
                     /inference="COORDINATES: protein motif:HMM:NF016230.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR6449051.1"
                     /translation="MYVCLCKAVTDKQIHEAVDAGVQTFTQLEESCGAGTGCGRCREV
                     AQELIDQRLADSAYYAA"
     gene            1887..2360
                     /gene="bfr"
                     /locus_tag="AC16QF_10620"
     CDS             1887..2360
                     /gene="bfr"
                     /locus_tag="AC16QF_10620"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HIR6449052.1"
                     /translation="MKPIDSDVIVHLNQVLRNELTAINQYFLHAKMFEDWGMHRIAAH
                     EKEESIDEMKHADALMERILFLEGLPNLQDLGKLYIGQDVKEILECDLKLELEAIPML
                     REAIAHCEHVGDYVSRQLFRDILSSEEEHVDWIETQLDLIAKTGVENYIQSQMQG"
     gene            2369..2674
                     /locus_tag="AC16QF_10625"
     CDS             2369..2674
                     /locus_tag="AC16QF_10625"
                     /inference="COORDINATES: protein motif:HMM:NF046888.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7009 family protein"
                     /protein_id="HIR6449053.1"
                     /translation="MKLRLSRAGLRLRLNGADLSALADGGTVTEPTPFPNGMFECSLT
                     AVADGIGACLTERGVAVTIPTASLGDLEAGNGLRAELQTSGEALHVLIEKDRRPEGR"
     gene            complement(2690..>2912)
                     /gene="mobA"
                     /locus_tag="AC16QF_10630"
     CDS             complement(2690..>2912)
                     /gene="mobA"
                     /locus_tag="AC16QF_10630"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HIR6449054.1"
                     /translation="EKTDAPVVVASDGEQRQNLHLLMKSDESARILSYLESGRRSVSG
                     WLDEVGAVVVEMPELAGSFKDYDSSDDLA"
BASE COUNT          576 a          858 c          928 g          550 t
ORIGIN      
        1 ggtgctggtc aactcctgcc tgacgctgtc tctggtgact ggtatgtcgg tctctgacgt
       61 cagccagaag acggtggcca atctgggctg gaacaacgtc aggctgagcg ggccggtgtt
      121 cgtcggcgac acgatttacg ccgagtcaga ggttttggag atccgggaat ccaaatcccg
      181 cccgaatcag ggcatcgtga cgatccgttc tcgcggcatc aagcaggacg gcacggaggt
      241 catcagcttc gagcgcgcca tgctcattcc ggcccggggc cacgccgtgg acgatgccat
      301 cgactattga ggcttgtgtc atggccgaac tctcagaaca ggaccggctc gtgctggact
      361 ccgtcgatcg gtttctcgat cgggacgtac gaccagtggc gcgcgcgctg gagcaggaag
      421 acgcctatcc tcacgagcta gtggagaaga tgaaggcgct cggcctgttc ggcgccacca
      481 tcgacgagcg tcatgggggg ctgggactga ccgcatccgt gtacgcgctc atcgtcgagc
      541 gcgtgtccgc cgtctggatg tctgtgtcgg gcatcttcaa ctctcatctg atcatgtgtg
      601 ccgccgtgca acgtttcggc accgaggcga tgaaggaacg ccttctcccg gcaatggcca
      661 cgggcgagct gcgagggggg atcgccctca ccgagccgga ctgtggaacc gacctgcagg
      721 ccattcgttg ccgtgcccgc cgcgacgggg agagctacgt gatcgacggg acgaagcagt
      781 ggatcaccaa cgcggtggaa ggcaacgtcc tggccgtgct ggtgaaaacg gatccggatg
      841 ccgttccggc tcacagaggc atgagcctgc tcatcgtcga aaaatccgcg ggttacaaag
      901 cgaccaagct caagaagctc ggctatcgcg gcgtggacac cggcgaggtg cagtttgatg
      961 gggtgcgggt gcccgcggcc aacctggtcg gcgatggtga gggccggggt ctgcagcaga
     1021 tcctctcggg actggcgttg ggccggatca acgttgcggc ccggggggtg ggggtggccg
     1081 atgcttcgct tcaggctgcc cttcgctacg cccaggagcg ccagacgttt ggcgtgccga
     1141 tccacgatca tcaggccatt cagatcaagc tggccgacat ggccacccgt gtggaagctg
     1201 cccggctgct cacggagaag gctgcccgaa tttacgatac gggacagcgc tgcgatctcg
     1261 aggcaggcat ggcgaagctc ttcgcatcgg aggccgcgct cagcaactcc ctggaagcga
     1321 tgcgcatcca tggtgcttac gggtactcgc ccgacaacga tgtcgagcgc tactatcgag
     1381 acgcgccgct catggcgata ggcgagggaa ccaacgagct gcagagaatc atcatcgccc
     1441 gccagctcat cgagcggaat cccatctgag aatgcggtcg ggccggcgga tcagtcaggc
     1501 agcgtagtac gccgagtcgg caaggcgctg atcgatgagt tcctgtgcca cctcgcggca
     1561 gcggccgcag ccggtgccgg ccccgcagga ttcctcgagc tgagtgaagg tctgaacgcc
     1621 ggcgtccacg gcttcgtgga tttgcttatc ggtaactgct ttacagaggc agacgtacat
     1681 ctcaattgag cgatcggatg aactgctgtt gatttaaatg caaatcattc tcggttgcaa
     1741 tagtttttgg ttctcgtttg taaccggatg gtattttcag atgggaatgc ttttcaatat
     1801 aaatcagtca cttagagaga ggcggccttg ctaagtccga ctcgctcccg catagtccgt
     1861 ctttcgaacc ggttcaggat cattccatga agcccatcga cagcgacgtc atcgttcacc
     1921 tcaatcaggt attgagaaac gagctcacgg ccatcaatca gtactttctc cacgcaaaga
     1981 tgttcgaaga ctgggggatg catcggatcg ccgctcatga gaaagaggag tccatagacg
     2041 agatgaagca tgccgatgcg ctgatggagc gcatcctgtt cctggaaggg ctgccgaacc
     2101 tgcaggacct cggcaagctc tacatcggtc aggacgtgaa ggagatcctc gagtgcgacc
     2161 taaaactcga actcgaagcg attcccatgc tgcgtgaggc catcgctcac tgcgagcacg
     2221 ttggcgacta cgtcagccga cagctctttc gcgacatcct gtcatcggag gaagagcacg
     2281 tagactggat cgaaacccag ctcgacctga tcgccaagac cggcgtcgag aattacatcc
     2341 agtcccagat gcagggctga gatcatcggt gaagctccgg ttgagcagag cgggccttcg
     2401 tcttcggctc aacggcgccg acctgtccgc actcgctgac ggggggacgg tgacggagcc
     2461 aacaccgttt cccaacggca tgttcgagtg cagcctgacg gcggtggcgg acggcatcgg
     2521 cgcgtgcctg accgagcgcg gcgttgccgt gacgataccg acggcctcgc ttggtgatct
     2581 ggaagccggg aacggcttga gggcggagct gcaaacgtcc ggcgaggcgc tccacgtgct
     2641 catcgagaaa gaccgccggc ccgagggccg ctgaggcgct cctgcccggt caggcgaggt
     2701 cgtcgctcga atcgtaatcc ttgaaagacc cggcgagttc gggcatctcc acaacgacgg
     2761 cgccgacctc atcaagccag cccgacacgc tgcgacggcc agattccagg tagcttagaa
     2821 tgcgcgcgct ctcatccgac ttcatgagca ggtggaggtt ctggcgctgc tcgccgtcgc
     2881 ttgccacaac cacaggagcg tccgtcttct cc
//
LOCUS       DBNHLM010000230         6915 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_990640, whole genome shotgun sequence.
ACCESSION   DBNHLM010000230 DBNHLM010000000
VERSION     DBNHLM010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6915)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6915)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6915
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_990640"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..692)
                     /locus_tag="AC16QF_10635"
     CDS             complement(84..692)
                     /locus_tag="AC16QF_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449055.1"
                     /translation="MKGVAEFVCGAGAGAGLALVALGLSGGAVAADAAPVSDWLQLNL
                     GHSAWLFALVLTVYVANLLRLSRLLAGGPDLKVVVKLDQLSDVWINLFVGIGVIWTAV
                     GMRSALQAALGDPGGALSGSAGTVLHKLVDGGILLALTTTIVGGVGGYVMRLVKTMVV
                     GARLQTFYSGVSRADLRELVGLGERIEARLARLDPQEPAVVA"
     gene            complement(693..1229)
                     /locus_tag="AC16QF_10640"
     CDS             complement(693..1229)
                     /locus_tag="AC16QF_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449056.1"
                     /translation="MFRRIFVPAFFISLVAGCTSTETVDACAVRFQDDLERSMSLAED
                     RLENGCEYRFDGYFQSLLAIAAENPDSRNPMLFSDHLMRVNDMGVISKRQAELLYNRY
                     FNVKFVSLRGDYNTCSQTCPMRDQVMSDMRAELHDKELGLMRASADRASFYRADTLLK
                     EADLVLEATCRACGADGR"
     gene            complement(1254..2087)
                     /locus_tag="AC16QF_10645"
     CDS             complement(1254..2087)
                     /locus_tag="AC16QF_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449057.1"
                     /translation="MDTNPRKGGSPLGRVLLPFLLLAAATASAVPDSGPVVRLFPTTV
                     LEDIRETGQVAKEMENSLQEVINRLDLQHQLYVESLCEGSDGDQGCERMAKQLGATYL
                     EMLTIMGDRLPEMELAVNNTRASLEKRLREELGHRTTPTSLQNALLGTTPASSAAAEQ
                     PALRGRSGVRLSDRFAQYYALVATQREGRGRSLAVVAADIYLDMEEASRLIAATQEEI
                     GRAALMEQLNQSFGLITPEMTEVVDGVKGILFGEASTDSPIAAPPYAIGESEFVSPLA
                     M"
     gene            complement(2150..2782)
                     /locus_tag="AC16QF_10650"
     CDS             complement(2150..2782)
                     /locus_tag="AC16QF_10650"
                     /inference="COORDINATES: protein motif:HMM:NF017269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HIR6449058.1"
                     /translation="MRAPALGLGLSLCVAAAPGMAGHSDGFPQAAPAGSHTHYTYADV
                     VGVEPLLEYRTVTEPRRDCRVAGYAAASSGTRSHRYTGPGHPDAHDHYQDHHDHGSPV
                     AAVIGGVVGGLLGSQFGSGSGKKAMTVAGALAGASVASSVSRSRSRHAGYTPRRCTTV
                     MTSREVEEVAGYRVRYVYQGEEFTKVVASDPGDRVRVQVQVTPAVGEPDW"
     gene            2976..4229
                     /locus_tag="AC16QF_10655"
     CDS             2976..4229
                     /locus_tag="AC16QF_10655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017905501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6449059.1"
                     /translation="MTKSDHHQILIIGGGAAGITVAASLKRRQGRHDLDIGIVEPSEH
                     HYYQPALTLVGAGTYPLEKTRRAESRLIPPGVAWHRDSATGFDPESNSVTLASGARLS
                     YDYLVVCPGLELNWERIDGLRETLGSNGVCSNYSPDHVEYTWQCLQSLQEGATALFTQ
                     APLPFKCPGAPQKIAYLAADHLQRRGLLGSCHLHFLTHGPALFAVPLFAAELSKVAER
                     WGIDVHFQTNLIRVDGPARQATFEKVGGEGEGEEVTMTFDMLHATPYQSPPAAVKESP
                     LANEAGWVDVHQHSMQHTTYANVFGLGDACSTPNSKTAAAVRKQAPVVVRNLLHLLDG
                     GSVEEAYDGYGSCPLTTAYGKVLLAEFTYGGKVTPTFPLDPRKERRSMWWVKKTALPI
                     MYWDYMLRGHEAFPGHDTEYEAPPV"
     gene            complement(4246..5133)
                     /locus_tag="AC16QF_10660"
     CDS             complement(4246..5133)
                     /locus_tag="AC16QF_10660"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6449060.1"
                     /translation="MARWDRDSGASIPDWFFEAVETEYATRSVEVDECDVVYQLWGDE
                     DKPGILLIHGMNAHSHWWDFIAPQLLDGYRVAAMNLTGMGDSDYRYEYDGATYAQEIR
                     AVCDHAGFDERAALVAHSFGGYMAVKAAVLFPDRFGALVLVDSGIRHPEEPVPDRPAM
                     GGRSKVYPDRETALGRFRVQPPQSCDNAYILEYIARHSLMPVDGGWAWKFDEDLPSTL
                     KDVERTPEDYRHLDVPTGLIYGAESELFSARTEDYMRELIAQDFPAVALPEAQHHLFL
                     DQPLAFVAELRKMLATLLA"
     gene            complement(5137..6267)
                     /locus_tag="AC16QF_10665"
     CDS             complement(5137..6267)
                     /locus_tag="AC16QF_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449061.1"
                     /translation="MDFSFSDEQTLLQDSIEKFIQNDYAFDARQRVVREEQGFSSDNW
                     RTFAELGWLGVPFPEADGGFGGTAIESMIMSEQFGKGLVVEPFLATVVLAGGALKIGG
                     SPSQKEKYLAGVMDGSLQGALAYAEPQGRFNLADLTTTASPEGDGYVINGYKAVVMNG
                     PSADFLVVSCRTGGGQRDADGVSLFVLPADTEGVARRDYPTVDAFRASEVTLENVRVG
                     SDALIGEEGKGLAILEQVVDEAILAVGAEAVGCMEVLYKTTVEYCKTREQFGQPIGKF
                     QVLQHRMVDMFMEHEQAKSLMYMAAMRLDEGYGPEAQKAVSAFKVQVGKSGRFVGQNA
                     VQLHGGMGMTEELNVGHYFKRLTMIDTLFGNVDFHLKRFGSL"
     gene            complement(6277..>6915)
                     /locus_tag="AC16QF_10670"
     CDS             complement(6277..>6915)
                     /locus_tag="AC16QF_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449062.1"
                     /translation="ISFLLIDMRSPGVEVHPIEILGGQHSVNSVRLDGVRVPVAHRVG
                     EENQGWTYAKALLSHERTGVARVATSGVQLNRVREIAEGQEPSADGLWQDAGFQRRYR
                     ELEIELIGLEMLELRILADLEAGQVPGPESSILKIKGTEIGQRINELMVEALGYYALP
                     YPDELLIDNEGPVGHDHALAALKGWLFSRAWSIYGGSNEVQKNIIARRALGL"
BASE COUNT         1239 a         2328 c         2182 g         1166 t
ORIGIN      
        1 gcaaaaggca atgatcgcca tgaaccgcat gacgtcagtc tgcaggccat cgccgtcggc
       61 ggcgtgattg accggctcga acgtcatgcc accaccgccg gttcctgggg gtcgagacgg
      121 gccagacggg cctcgatccg ctcgccaagg cccaccagct cgcgaaggtc tgcacggcta
      181 acgccggagt agaaggtctg caggcgggcg ccgaccacca tcgtcttgac cagacgcatg
      241 acgtagcctc cgaccccgcc gacgatggtc gtcgtcaacg ccagcagaat acctccgtcg
      301 acgagcttgt gaaggaccgt gcctgcggag cccgacagcg cgccgccggg atcaccaagg
      361 gccgcctgca gcgcgctgcg catgccgacg gccgtccata tgaccccgat accgacgaac
      421 aggttgatcc agacgtcgga gagctgatcg agcttgacga ccaccttcag gtccgggccg
      481 ccggcgagca gtcggctcag ccggagcagg ttggcgacat agaccgtcag caccagagcg
      541 aagagccagg cggagtgccc caggttgagc tgcagccagt cgctcacggg tgcagcgtcc
      601 gcggcaacag ccccgcccga gaggcccagg gccaccagcg cgagccccgc gcccgcgccc
      661 gctccgcaca cgaactccgc gacgcctttc attcagcgcc cgtccgcgcc gcaagcccga
      721 caggtcgctt ccaggacgag atcggcttcc ttgagcaggg tgtccgcccg gtagaagctc
      781 gcccggtccg cgctggcccg catcaggcca agctccttgt cgtgcagctc cgcgcgcatg
      841 tcggacatga cctgatcgcg catggggcag gtctgcgagc acgtgttgta gtcgccgcgc
      901 agggagacga acttgacgtt gaagtaacgg ttgtagagca actccgcctg gcgcttgctg
      961 atgacgccca tgtcgttcac gcgcatcagg tgatcgctga agagcatggg attcctcgag
     1021 tccggatttt ccgcggcgat cgccagcagg gactgaaagt agccatcgaa gcggtactcg
     1081 catccgttct ccagccgatc ctcggcgagg ctcatgctgc gctcgaggtc gtcctgaaat
     1141 cgcaccgcac aggcatccac ggtctccgtg ctggtgcagc cagcgacgag cgagatgaaa
     1201 aaggccggaa caaatatcct acgaaacatg tctttctcct gatcgaatcg gcgctacatg
     1261 gccagcgggc tgacgaactc ggactcgccg atggcgtagg ggggcgccgc aatggggctg
     1321 tcggtggaag cctcgccgaa caggatgccc ttcacgccgt cgaccacttc ggtcatctcc
     1381 ggcgtgatca gcccgaacga ctggttgagc tgctccatca gcgccgcgcg tccgatttcc
     1441 tcctgggtcg cggcgatgag gcgggaagct tcttccatgt ccagatagat gtcggcggcg
     1501 accaccgcca gcgagcggcc acggccctca cgctgggtcg cgaccagggc gtagtactgt
     1561 gcaaacctgt cggaaagccg caccccggaa cggccgcgaa gcgccggctg ctcggcggcg
     1621 gcgctgcttg caggcgtggt ccccagcagg gcgttctgca gcgatgtggg ggtggttcga
     1681 tgcccgagct cttcccgcag gcgcttttcc aggctcgcgc gggtattgtt tacggccagt
     1741 tccatctccg gaagccggtc acccatgatg gtgagcatct ccagataggt cgcgccaagc
     1801 tgtttggcca tgcgctcgca gccctggtcg ccgtcggagc cctcgcagag gctttccacg
     1861 tatagctgat gctgcagatc gagccggttg atgacctcct gcaggctgtt ctccatttct
     1921 ttggcaacct ggcctgtctc acgtatgtcc tcgagcacgg ttgtcgggaa gagccgaacg
     1981 accgggcccg aatccgggac ggcggaagcc gtggcggcgg caagcagcag gaacggcagc
     2041 agcacgcggc cgagcgggct gccgcccttt cttgggttgg tatccatggg catatcctct
     2101 ttgcttgcgc gccgcaaaac ggcgcaggtg tctggacgga agagagcgct caccaatctg
     2161 gctccccgac ggcgggagtg acctgaacct gaacgcgcac gcgatcgccc gggtctgaag
     2221 ccaccacctt ggtgaactct tctccctgat agacgtagcg cacccggtag ccggcgacct
     2281 cttccacctc gcgggacgtc atgaccgtcg tgcagcgccg cggcgtgtag ccggcatggc
     2341 gcgaccggga ccggctgacg ctgctggcaa ccgaggcccc cgccagcgct ccggcaacgg
     2401 tcatcgcctt cttgccgctg ccgctgccga actggctgcc cagcagcccg cccacgacgc
     2461 cgccgatcac ggcggcaacc ggactgccgt gatcgtggtg atcctggtag tggtcgtggg
     2521 cgtccggatg gccaggtccg gtgtatcggt gcgaacgggt gccggaacta gccgcggcgt
     2581 accccgcaac ccgacaatcc cttcggggct cggtgaccgt gcgatattcc aacaggggct
     2641 ccacgccgac gacgtcggcg taggtgtagt gagtgtggga tccggcgggg gcggcttgcg
     2701 gaaaaccgtc gctgtggccc gccatgccgg gtgccgcggc aacgcagaga ctcaagccga
     2761 ggccaagggc tggtgcgcgc atgtttcctc cggaactccc aaatctgtcg agactcgaat
     2821 gttatgggag ctcagctaaa cagaagctga acggggcggc ccgaccgggg cgggtttgcc
     2881 ctaccgggcg cacccgcttc tccagccccc cggagcggcg tatgctcatc cagactccag
     2941 cggcaccgct cgcaggaccc ggccggagag ccaacatgac caagtcagac catcatcaga
     3001 tcctgatcat cggcggcggg gcggcaggaa ttacggttgc cgccagcctc aagcgcagac
     3061 agggacggca cgacctcgac atcggaatcg tcgagccgtc cgagcatcac tactaccagc
     3121 cggcgctgac gctcgtcggc gcgggcactt accctctcga gaagacccgc cgggcggagt
     3181 cgcggctgat cccgccaggc gtggcgtggc atcgggactc ggcaaccggt ttcgatccgg
     3241 agtccaactc ggtgaccctg gcctccgggg cgaggttgtc ctacgactac ctggtggtct
     3301 gcccgggcct ggagctcaac tgggagcgaa ttgacggtct ccgggaaacc ctggggagca
     3361 acggcgtctg cagcaactac tcgcccgacc acgtcgagta cacgtggcaa tgcctgcagt
     3421 cacttcagga aggcgccacc gcgctgttca ctcaagcgcc actgccgttc aagtgcccgg
     3481 gagcgcctca gaagatcgcc tacctggccg ccgatcatct gcagcgccgc gggctgctcg
     3541 gaagctgcca tctgcacttt ctcacccacg gccccgctct gtttgcggtg ccgctgttcg
     3601 cagcggagct gagcaaggtg gccgagcgct ggggcatcga cgtccatttt cagaccaacc
     3661 tgatcagggt ggacgggccc gcacggcagg ccaccttcga aaaggtcggc ggcgaaggag
     3721 agggagaaga ggtcaccatg accttcgaca tgctgcacgc aaccccgtat cagagcccgc
     3781 ccgcggccgt gaaggagagc cccctggcca acgaggcagg ctgggtcgac gtgcaccagc
     3841 acagcatgca gcacaccacc tacgccaacg ttttcgggct gggggacgcc tgctcgaccc
     3901 caaactccaa gacggcggct gccgtgcgca agcaggcgcc ggtggtggtg cgcaatctgc
     3961 tccacctgct cgacggcggc agcgtcgagg aagcttacga cggctacggc tcgtgccccc
     4021 tgaccaccgc gtacggcaag gtcctgctgg ccgagttcac ctacggggga aaggttacgc
     4081 cgaccttccc gctggacccg cgcaaggagc gaagaagcat gtggtgggtg aagaagacgg
     4141 cgctgccgat catgtactgg gactacatgc tgcgaggtca cgaggcgttc ccgggccacg
     4201 acacggagta cgaagcgccg ccggtgtgag cgggctaggg cgagttcagg cgagaagcgt
     4261 cgccagcatc ttcctcagct cggcgacgaa cgccagcggc tggtccagga acagatggtg
     4321 ctgtgcttcc ggcagcgcca cggccgggaa gtcctgggca atgagctcgc gcatgtagtc
     4381 ttccgtgcga gccgagaaca gctcgctttc cgctccgtag atgaggcccg tgggcacgtc
     4441 cagatgccgg tagtcttccg gcgtgcgctc gacatccttt agagtcgacg gcaggtcctc
     4501 gtcgaacttc caggcccatc cgccatccac gggcatgagc gaatgacgtg cgatgtactc
     4561 gagaatatag gcgttgtcgc agctctgcgg gggctgcacg cgaaagcggc cgagcgcggt
     4621 ctcccggtcg ggatacacct ttgaccgccc tcccatcgcc ggcctgtccg gaaccggctc
     4681 ctccggatga cggattccgg aatccaccag gaccagtgcc ccgaaacgat cgggaaagag
     4741 cacggccgct ttgacggcca tgtaaccgcc gaagctgtgc gccaccaggg ctgcacgctc
     4801 gtcgaacccc gcatggtcac agacagccct gatctcctgg gcgtaggtgg cgccgtcgta
     4861 ctcgtagcgg tagtcggagt cgcccatgcc cgtgaggttc atggcggcga cccgatagcc
     4921 gtcgagcagc tgaggcgcga tgaagtccca ccagtgggag tgggcgttca tgccgtggat
     4981 cagcaggatc ccgggtttat cctcgtcgcc ccatagctgg tacaccacgt cgcactcatc
     5041 gacctcgact gaccgggtcg cgtactcggt ctccaccgcc tcgaagaacc agtcgggtat
     5101 ggaggccccg gagtcacgat cccagcgagc cacgatctaa agcgacccga accgcttgag
     5161 gtggaagtcc acgttgccga acagcgtgtc gatcatggtg aggcgcttga aatagtggcc
     5221 tacgttgagc tcctcggtca ttcccatccc gccgtgcagc tggacggcgt tctggccgac
     5281 gaacctgccc gacttgccca cctgaacctt gaacgccgat acggctttct gcgcttccgg
     5341 gccatagccc tcgtccaggc gcatggccgc catgtacatc agagacttgg cctgctcgtg
     5401 ctccatgaac atgtccacca tgcgatgctg cagcacctga aacttgccga tgggctgacc
     5461 gaactgctcg cgggttttgc agtactccac cgtcgtcttg tagagcacct ccatgcagcc
     5521 cacggcttcc gcgccgacgg ccaggatggc ctcgtcgacg acctgctcga gaatggcgag
     5581 cccctttcct tcctcgccga tcaacgcatc gcttcccacc cggacgttct ccagcgtcac
     5641 ctcggacgcc cggaaggcat ctaccgtggg gtagtcgcgc ctggccacgc cttcggtatc
     5701 cgcaggcagc acgaagaggc tgacgccatc cgcatcccgc tggccgccgc ccgtgcggca
     5761 ggatacgacc agaaagtcgg cggacgggcc gttcataacc acggctttgt aaccgttgat
     5821 cacgtagccg tcaccctccg gggacgccgt ggtggtgaga tcggcaaggt tgaatcgtcc
     5881 ctggggctcc gcgtacgcca gggcaccctg cagcgatccg tccatgacgc cggcgaggta
     5941 tttctccttc tgcgacgggc tgccgccgat cttcagcgcg cccccggcga gaaccaccgt
     6001 cgccaggaag ggctccacga ccagcccttt gccgaactgc tccgacatga tcatcgactc
     6061 gatggccgtt ccgccgaagc cgccgtcggc ttccgggaac gggaccccga gccagccgag
     6121 ctcggcaaac gtccgccagt tgtcgctgga gaaaccctgc tcctcccgaa ccacccgctg
     6181 gcgcgcgtcg aacgcgtagt cgttctggat gaacttctcg atgctgtcct gcagcagcgt
     6241 ctgctcgtcg gaaaaagaaa aatccataac ttcccctcaa agacccagcg cccgccgggc
     6301 gatgatgttt ttctgaactt cgttcgaccc gccgtaaatg ctccaggcgc ggctgaacag
     6361 ccaacccttg agcgcggcga gcgcgtgatc gtgacctacc gggccttcgt tgtcgatcag
     6421 caactcatcg ggatagggca gcgcgtagta gccgagggcc tccaccatca actcgttgat
     6481 gcgctggccg atctccgtgc ccttgatctt gagaatggac gactccggcc ccgggacctg
     6541 gcccgcctcg aggtctgcaa gaatgcgcaa ctccagcatc tcgaggccaa tcagctcgat
     6601 ttcgagttcc cgatagcgac gctggaagcc tgcgtcttgc cagagaccgt ctgcgctcgg
     6661 ttcctgccct tcggcaatct cccgtacgcg attgagctgc actccagacg tcgcgacccg
     6721 ggccacccct gtccgctcat ggctcagaag agctttggcg taggtccagc cctggttttc
     6781 ctcgcccaca cgatgcgcga ccgggacgcg gacaccgtcc agccggacgc tgttcacgct
     6841 gtgctggccg ccgaggatct cgatgggatg aacctcgaca cccggcgagc gcatgtcgat
     6901 gagcaggaag ctgat
//
LOCUS       DBNHLM010000231        11640 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1288754, whole genome shotgun sequence.
ACCESSION   DBNHLM010000231 DBNHLM010000000
VERSION     DBNHLM010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11640
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1288754"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3890)
                     /gene="rpoC"
                     /locus_tag="AC16QF_10675"
     CDS             complement(<1..3890)
                     /gene="rpoC"
                     /locus_tag="AC16QF_10675"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR6449063.1"
                     /translation="MKDLLNMLKAQSSVDEFDSLRIGLASPEMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTLTKVRRERM
                     GHIELASPVAHIWFLKSLPSRIGLLLDMTLRDIERVLYFESYVVVDPGLTDLEIGQLL
                     TDEDYYQKLEEYGDEFDAKMGAEAIQALLLNLDLDSEIARLREEIPATNSDTKIKKLS
                     KRLKLMEAFVNSGNRPQWMIMTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLSAPDIIVRNEKRMLQESVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVIVVGPTLRLHQCGLPKKMALELFKPFIFGKLEARGLAT
                     TIKAAKKMVERETPEVWDILAEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCTAYNADFDGDQMAVHVPLTLEAQLESRALMMSTNNILSPASGEPIIVPSQDVVL
                     GLYYMTREMVNVPGEGMAFSDIEEVHRAYYAGRVDLHARIKVRIEEYVQRDDGSYEAV
                     TSLAETTVGRALFYEIVPKALPFELVNKPLDKKAISTLINVCYRNVGLKDTVIFADQL
                     MYTGFAHSTSSGASIGVDDFVIPEAKTKIIEEAEAEVAEIESQYASGLVTQGEKYNKV
                     VDIWSRANDLVARSMMDGISKETVVGVVRDEHGKVVRSGGKPKTEEVEQDSFNSVFIY
                     ADSGARGSPAQIRQLAGMRGLMTRPDGSIIENAITANFREGLSVNEYFISTHGARKGL
                     ADTALKTANSGYLTRRLVDVAQDVVITEVDCGTDKGLRAAAIIEGGDVVEPLGDRVLG
                     RVVAQDVFDVDAKTLIIPGGTMLDEAWVERLDILGIDEILVRSAITCSTRHGVCGACY
                     GRDLARGHQVNVGEAVGVIAAQSIGEPGTQLTMRTFHIGGAASRATAIDNIQVKHGGS
                     VRLHNLKTVERQSGELVAVSRSGEIAVADEHGRERERYKLPYGAVISVADSDSVEAGQ
                     VIAQWDPHTHPIVTEVAGTVQFQDMEEGLSINRQTDELTGLTNITVLDPKDRPSSGKD
                     LRPAVSLVDGKGKEVVLPGSDQPAHYFLPSDAILNLENKDKIGVGDVIARIPQEGSKT
                     RDITGGLPRVADLFEARKPKEPAILAEQTGTVSFGKETKGKRRLVVTPADGDPVETLI
                     PKWRNIGVFEGESVERGEVVCDGPPSPHDILRLKGVAELAKYIINEIQEVYRLQGVTI
                     NDKHIEVIVRQMLRKAEIVDPGESELIRGEQLEY"
     gene            complement(3961..8034)
                     /gene="rpoB"
                     /locus_tag="AC16QF_10680"
     CDS             complement(3961..8034)
                     /gene="rpoB"
                     /locus_tag="AC16QF_10680"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR6449064.1"
                     /translation="MAYSFTEKKRIRKDFGKLSEMMEMPYLLSIQVDSYDRFLQLAAP
                     AEGRDDIGLHAAFRSVFPIVSYSGNAALEYVDYRLGQPAFDVKECVLRGTTYAAPLRV
                     KVRLIIYDKESSNKAVKDVKEQEVYMGEIPLMTDNGTFVINGTERVVVSQLHRSPGVF
                     FDHDRGKTHSSGKLLYSARVIPYRGSWLDFEFDPKDCVFVRIDRRRKLPATIILRALD
                     FSAEEILGMFFDKNIFHVKKDGFSVDLIPERMRGDVATFDITDRQGNVIVELGRRITA
                     RHVRLLEDSGLKRLDVPREYMLDRVIAADVVDESTGEIVVACNSIITDEVLDKLLETG
                     VSDIETIYTNDLDCGPYISETLRIDPTSNRLEALVEIYRMMRPGEPPTKEAAENLFTN
                     LFFSAERYDLSGVGRMKFNRRLGREDITGEGILTNEDIIDVLRTLIDIRNGRGSVDDI
                     DHLGNRRVRSVGEMAENQFRVGLIRVERAVTERLSLAESEGLMPQDMINAKPVAAAVK
                     EFFGSSQLSQFMDQNNPLSEVTHKRRVSALGPGGLTRERAGFEVRDVHPTHYGRVCPI
                     ETPEGPNIGLINSLATYARTNEYGFLETPYRRVKGGKVAQEIEYLSAIDEAQYVIAQS
                     SSPLDATGAFEGDLVDVRHQNEFTKTSAENVNYMDVSPKQVVSVAASLIPFLEHDDAN
                     RALMGSNTQRQAVPTIRTEKPLVGTGMERHVARDSGVCVIASRGGTVDSVDAGRIVVR
                     VADAETEAGEAGVDIYNLTKFTRSNQNTCINQRPLVKPGDVIARGDILADGPSCDMGE
                     LALGQNMRIAFMPWNGYNYEDSILVSERVVQEDRFTSVHIQELTCVARDTKLGPEEIT
                     CDIPNVGEGALSKLDESGIVYIGAEVGAGDILVGKVTPKGETQLTPEEKLLRAIFGEK
                     ASDVKDTSLRVPTSVKGTVIDVRVFTRDGVEKDQRAKDIEQSELAQHRKDLNDEYRIV
                     ETATYERLRRALLGKKAASAPGKSKGGTVDEALLDGLGNDDWFKIRMADDALNAQLER
                     AEAQLMERKARLDAVYADKRSKLESGDDLAPGVLKIVKVYLAIKRHIQPGDKMAGRHG
                     NKGVISVIMPVEDMPYDEHGEPVDIVLNPLGVPSRMNVGQVLETHLGWAAKGIGEKIG
                     EMLNAQRKVAEVREFVERVYNAVGERKEDIGSLSDAEVIELAGNLRGGIPIATPVFDG
                     AAESEIKSMLELAELPLSGQATLYDGRTGDKFDRPVTLGYMYMLKLNHLVDDKMHARS
                     TGSYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVTGRTKMY
                     KNIVDSDYLMEPGMPESFNVLVKEIRSLGIDIELESE"
     gene            complement(8186..8569)
                     /gene="rplL"
                     /locus_tag="AC16QF_10685"
     CDS             complement(8186..8569)
                     /gene="rplL"
                     /locus_tag="AC16QF_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003176432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR6449065.1"
                     /translation="MALSKDDILNAIADMSVMEVVELVQAMEEKFGVSAAAAVAVGPV
                     AAAAEGGDGAAEEKDEFDVILSGAGEKKVNVIKAVRAITGLGLKEAKALVDEAPSPIK
                     EGAGKDEAEEIKKQLEEAGASVELK"
     gene            complement(8615..9163)
                     /gene="rplJ"
                     /locus_tag="AC16QF_10690"
     CDS             complement(8615..9163)
                     /gene="rplJ"
                     /locus_tag="AC16QF_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019693882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR6449066.1"
                     /translation="MALRLEDKQAIVAEVNQAATTALAAVLSDYRGLTVSEMTELRAK
                     ARSSGVYLKVIRNTLAKRAIEGTEYECLNEAFVGPTMVAFSQEDPGSAARLIKDCAKE
                     YDALKVKALSIGGQVYGPEDIDRVAKLPTRDEALALLMSVMQAPITKLARTFNEVPGK
                     LVRTVAAIRDQKQAGGDAAAEG"
     gene            complement(9458..10153)
                     /gene="rplA"
                     /locus_tag="AC16QF_10695"
     CDS             complement(9458..10153)
                     /gene="rplA"
                     /locus_tag="AC16QF_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003232403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR6449067.1"
                     /translation="MAKMTKRMRAIAEKVDREKSYPIEEAVELLGSLAKAKFKESFDI
                     SVNLGVDPRKSDQVVRGATTLPHGTGKEVRVAVFAQGESADAATAAGADIVGFEDLAE
                     KVKAGDLNFDVVIATPDAMRVVGQLGKILGPRGMMPNPKTGTVTPDVATAVANAKAGQ
                     VQFRTDKAGIIHGSVGQVGFEAEHIRENVKALVQDLNKAKPASAKGVYLKKITLSTTM
                     GPGVAIDQASLEV"
     gene            complement(10153..10587)
                     /gene="rplK"
                     /locus_tag="AC16QF_10700"
     CDS             complement(10153..10587)
                     /gene="rplK"
                     /locus_tag="AC16QF_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR6449068.1"
                     /translation="MAKKIDAYIKLQVAAGQANPSPPVGPALGQHGVNIMDFCKAFNA
                     QTQSLEQGLPTPVVITVYADRSFTFVTKTPPAAVLLRKAAKLAKGSPNPNTQKVGKVN
                     RSQLEEIAQSKMPDLTAADMDAAVRTIAGTARSMGIEVEGVN"
     gene            complement(10663..11196)
                     /gene="nusG"
                     /locus_tag="AC16QF_10705"
     CDS             complement(10663..11196)
                     /gene="nusG"
                     /locus_tag="AC16QF_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR6449069.1"
                     /translation="MSKKWYVVHAYSGFEKNVARALKERVELSSLGDHFGEILVPTEE
                     VVEMRGGQKRRSERKFFPGYVLVEMELNDDTWHLVKETPRVMGFIGGKADQPAPLSEA
                     EAAAILDRVAAGSDKATPKTIFEPGEIVRVIDGPFNDFNGVVEEVNYEKSRLQVAVTI
                     FGRSTPVELDFGQVEKG"
     gene            complement(11204..11539)
                     /gene="secE"
                     /locus_tag="AC16QF_10710"
     CDS             complement(11204..11539)
                     /gene="secE"
                     /locus_tag="AC16QF_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004397083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR6449070.1"
                     /translation="MDWLKWLVVAVLVGGGVFGNWYYQDESLLLRVGGLIAMAVAAVM
                     IAVQTEKGRTTWSLMKEARSEIRRVVWPTRDEVLQTTMIVLVLVLVFALLLWLLDSGL
                     SWLVSSVIG"
BASE COUNT         2059 a         3790 c         3392 g         2399 t
ORIGIN      
        1 ttgtactcca gctgctcgcc gcggatgagc tcagattcgc ccggatcaac gatctccgcc
       61 ttgcgcagca tctgccgcac gatgacctca atgtgcttgt cattgatggt cacaccctga
      121 agccgataga cctcctggat ctcgttgatg atgtacttgg ccagctcggc gacgcccttc
      181 agacggagaa tgtcgtgggg gcttggtggg ccatcgcaga ccacctcccc gcgctcgacg
      241 ctctcaccct cgaacacacc gatgttgcgc cacttcggaa tcagcgtctc gacggggtcg
      301 ccgtctgcag gcgtgaccac caggcgtcgc ttgcccttgg tctccttgcc gaagctgacg
      361 gtacccgtct gctcggcgag gatcgccggt tccttgggct tgcgggcctc gaacagatcg
      421 gcaaccctcg gcaagccacc cgtgatgtcc cgggttttgg acccttcctg aggaatacgg
      481 gcgatgacat cgcccacccc gatcttgtcc ttgttctcca ggttgaggat ggcgtcgcta
      541 ggcagaaaat aatgcgcagg ctggtcgctt cccggcagaa ccacctcttt gcctttgccg
      601 tcgacgaggc tcaccgcagg ccgcagatcc ttgccggagc tgggccgatc cttgggatcg
      661 agcacggtga tgttggtcag gccggtcagc tcgtcggtct ggcggttgat ggacagacct
      721 tcttccatgt cctggaactg aaccgtgccg gccacctcgg tgacgatggg gtgagtatga
      781 ggatcccact gggcgatcac ctgcccggcc tcgacgctgt cgctgtcggc cacggagatc
      841 acggccccat aggggagctt gtaacgttca cgctcacggc catgctcgtc ggcaacggca
      901 atctcgccgg accgggaaac cgcaaccagc tcgccgcttt gcctctccac ggtcttcagg
      961 ttgtgcagcc gaacggaacc cccgtgcttg acctggatgt tgtcgatggc ggtggcgcgg
     1021 gatgcggctc cgccgatatg gaacgtccgc atggtcaact gggtgcccgg ctcgccgatg
     1081 gactgagcgg cgatgacacc cacagcctcg ccaacgttca cctggtgacc gcgggcaagg
     1141 tccctgccgt agcatgcgcc gcacaccccg tgccgcgtag agcaggtgat ggcggagcgc
     1201 acgaggatct cgtcgatccc cagtatgtcc aggcgttcca cccatgcctc atcgagcatg
     1261 gtgcctccgg gaatgatcag ggtctttgcg tccacatcga aaacgtcctg agccactacc
     1321 cgaccgagga cccggtcgcc caggggctcg acgacgtcac caccctcgat gatggctgcg
     1381 gccctcagcc ctttgtcggt tccgcagtcg acctcggtga tgactacgtc ctgagcaacg
     1441 tcgaccagcc ggcgggtgag ataccctgag ttagcggtct tcaaggcggt gtccgcaaga
     1501 cccttgcggg ccccgtgtgt cgagatgaag tactcgttca ccgaaagccc ttcccggaag
     1561 ttcgcggtga tcgcattctc gatgatgctg ccgtcaggac gggtcatgag tccgcgcatg
     1621 ccggcaagct gacggatctg cgcgggagaa ccacgggcgc cggagtcggc gtagatgaag
     1681 acggagttga acgagtcctg ctccacttcc tccgtcttcg gcttaccgcc ggacctcacc
     1741 accttgccgt gctcgtcgcg cacgacgccg acgacggttt ccttggagat gccgtccatc
     1801 atggagcgcg ccaccagatc gttggctctc gaccagatgt ctacgacctt gttgtacttc
     1861 tcgccctggg tcaccagacc cgaagcgtac tgcgactcga tctccgcaac ttcggcttcg
     1921 gcctcttcaa tgatcttcgt cttggcctcc ggtatgacga aatcgtcgac gccgatggac
     1981 gcgcccgagg acgtcgagtg cgcaaagccg gtgtacatga gctggtccgc aaagatgacc
     2041 gtgtccttca ggcccacgtt gcggtagcag acattgatca gcgtggatat ggccttcttg
     2101 tcgagcggct tgttgaccag ctcgaagggc agcgccttgg gaacgatctc gtagaacagc
     2161 gcccggccga cggtggtctc cgcgaggctc gtgacggcct cgtagctgcc atcatcccgt
     2221 tggacgtact cttctatccg caccttgatt ctggcatgca gatccacccg gccggcgtaa
     2281 taggcacgat gcacctcctc gatgtccgag aacgccatgc cttcgcccgg gacgttcacc
     2341 atctcccggg tcatgtaata gagcccgagc accacgtcct gagagggcac gatgatcggc
     2401 tcgccgctcg ctggagagag gatgttattg gtggacatca tcagagcacg gctttcgagc
     2461 tgagcttcca gcgtcagcgg cacgtgaacg gccatctggt ccccgtcgaa gtcggcgttg
     2521 taagccgtgc agacgagcgg gtgcagctga atggccttgc cttcgatgag caccggctcg
     2581 aacgcctgaa tgcccagacg gtggagcgtg ggggcccggt tcagcagcac cggatgctcg
     2641 cggatgactt cagccaggat atcccatacc tccggcgtct cccgctcgac catcttcttg
     2701 gcagccttga tggtcgtggc aagaccccgg gcttccagct tgccgaaaat gaacggcttg
     2761 aagagctcca gggccatctt cttgggcagc ccgcactgat gcagccgcag ggtcggaccc
     2821 accacgatca ccgaacgtcc cgagtaatct acccgtttgc caagcaggtt ctgtcggaac
     2881 cggccctgct tgcccttgat catgtcggca agcgacttca gcgggcgctt gttggaaccg
     2941 gtgatcgccc ggccgcgccg gccgttgtcc agcagcgcgt cgacggactc ctgcaacatg
     3001 cgtttctcgt tgcggacgat gatgtccggc gcggagaggt ccagcagccg cttcaggcgg
     3061 ttgttgcggt tgatcacgcg ccggtagaga tcgttgaggt ccgaggtcgc gaagcgaccg
     3121 ccgtccaggg gcaccagggg acgcaggtcc ggcggcagca ccgggagcac cgtcatgatc
     3181 atccactggg gacggtttcc ggagttcacg aacgcttcca tgagcttcag gcgcttggag
     3241 agcttcttga tcttggtgtc gctgttggtc gccgggatct cctcgcgcag gcgagcgatc
     3301 tccgagtcca gatccagatt cagcagaagg gcctgaatgg cttccgctcc catcttggca
     3361 tcgaactcgt caccgtactc ctcgagcttc tggtaatagt cttcgtccgt gagcagctgg
     3421 ccgatctcca gatccgtgag gcccgggtcg acgaccacgt aggactcgaa atagagaacc
     3481 cgctcgatgt cgcgcagggt catgtccagc agcaggccaa tgcgtgacgg cagggacttc
     3541 agaaaccaga tgtgcgccac cgggctcgca agctcgatgt gccccatgcg ctcgcggcgg
     3601 accttggtga gggtcacctc gacgccgcac ttctcgcaga tcacgccacg gtgcttgagc
     3661 cgcttgtact tgccgcagag gcactcgtaa tccttgatcg gcccgaaaat ccgggcgcag
     3721 aagagcccat cgcgctcagg cttgaacgtg cggtagttta tggtctccgg cttcttcacc
     3781 tcgccgaaag accacgaccg gatcatctcc ggggatgcca gaccgatacg caacgagtca
     3841 aactcgtcga cgctgctctg ggccttcaac atattgagta gatctttcaa gttctctcct
     3901 cccggtcgga tttgagtagc acgctaccga tttcgtcaca ggtcattggc tgacaggaat
     3961 tcattcgctc tccagctcga tgtcgatgcc cagggagcgg atctccttga cgagcacgtt
     4021 gaacgattcc ggcatgcccg gctccatgag gtaatcgctg tcgacgatgt tcttgtacat
     4081 cttcgtgcga ccggtgacgt cgtccgactt caccgtgagc atctcctgga gcgtgtacgc
     4141 ggccccgtag gcctcgaggg cccagacttc catttcgccg aagcgctgcc cgccgaactg
     4201 cgctttgccg cccaacggct gctgggtcac caggctgtag gagcccgttg atctggcatg
     4261 catcttgtcg tcgacgagat ggttgagctt gagcatgtac atgtagccga gagtaacggg
     4321 ccggtcgaac ttgtcgcccg tacgcccatc atacagggtc gcctggccgc tgagcggaag
     4381 ctccgccaac tccagcatgc tcttgatctc gctctcagca gcgccatcga acaccggggt
     4441 ggcaatgggg atgccgcccc ggagattccc ggcaagctcg atcacctcgg catccgaaag
     4501 cgaaccgatg tcttccttgc gttcgcctac ggcattgtat acccgctcga cgaactccct
     4561 gacctcggcc accttgcgct gcgcgttgag catctcgccg atcttctcgc cgatgccctt
     4621 cgctgcccat ccaagatggg tctcgagcac ctgaccgacg ttcattcgcg agggaacgcc
     4681 aagcgggttg agaactatgt cgacgggctc accgtgctcg tcgtacggca tgtcctccac
     4741 cggcatgatc accgagatca cccctttgtt cccgtgcctg ccggccatct tgtcgccggg
     4801 ctgaatgtgt cgcttgatcg ccagatacac cttcacgatc ttgagcacgc cgggagccag
     4861 gtcgtcgccg ctttcgagct tggagcgctt gtctgcatag acggcatcca gacgggcctt
     4921 gcgctccatg agctgcgcct cggctcgctc gagctgggcg ttcagggcgt catcagccat
     4981 ccggatcttg aaccagtcgt cgttgccgag accgtcgagc agcgcttcgt ccaccgtgcc
     5041 gcccttgctc ttgcccggag cgctagccgc cttcttgccg agcagagccc gccgcaagcg
     5101 ctcgtaggtc gcggtctcga caatgcggta ctcatcgttg agatccttgc ggtgctgcgc
     5161 aagctcggac tgctcgatgt ccttggcccg ctgatccttc tccaccccgt cccgggtgaa
     5221 tacccggacg tcgatgacgg tgcccttgac gctcgtcggg actcgaagcg acgtgtcttt
     5281 cacgtccgat gctttctcgc cgaagatggc acgcagcagc ttctcttcag gggtcagctg
     5341 ggtctcgccc ttcggggtga ccttgccgac gagaatgtcg ccggcaccaa cctcggcgcc
     5401 gatgtagacg atccccgact cgtccagctt ggagagcgca ccctcgccga cgttgggaat
     5461 gtcgcaggta atctcctcgg gtccgagttt ggtatcccgg gcaacacagg tgagttcctg
     5521 gatgtggacg ctggtgaagc ggtcctcctg gacaaccctt tcggacacga ggatggagtc
     5581 ctcgtagttg tatccgttcc agggcatgaa cgcgatgcgc atgttctggc ccagagccag
     5641 ctcacccata tcgcacgacg ggccgtccgc gagaatgtct ccgcgggcga tcacgtctcc
     5701 cggcttcacc agcggacgct ggttgatgca ggtgttctgg ttggatcggg tgaacttggt
     5761 caggttgtag atgtcgaccc cggcttcacc cgcctcggtc tccgcgtcgg ccacgcgcac
     5821 cacgatgcgg cccgcgtcca cgctgtccac ggtgcccccg cggctggcga tgacgcagac
     5881 gccggaatct ctggccacgt gccgttccat ccccgttccg accagcggct tttcggtgcg
     5941 aatggtcgga accgcctgac gctgcgtgtt cgagcccatg agggcccggt tggcatcatc
     6001 gtgctccaga aacggaatca gcgaggccgc cacggatacg acctgcttgg gcgagacgtc
     6061 catgtagttc acgttttccg ccgaagtctt ggtgaactcg ttctgatggc gcacgtcgac
     6121 gagatcgccc tcgaacgcac ccgtggcgtc cagaggagaa ctcgactggg cgatcacgta
     6181 ctgcgcctcg tcgatggccg acaggtactc gatctcttga gcgaccttgc cgcccttgac
     6241 ccggcggtag ggcgtctcga ggaaaccgta ctcgttcgtg cgggcgtaag tcgccagcga
     6301 attgatgagg ccgatgttcg ggccttccgg cgtctcgatt gggcagacgc gcccgtaatg
     6361 ggtcgggtgg acgtcgcgca cctcgaagcc tgcacgctcg cgcgtcaggc cccccgggcc
     6421 aagcgccgaa acgcggcgct tgtgagtcac ctcggacagc gggttgttct gatccatgaa
     6481 ctgcgacagc tggctggaac cgaagaactc cttgaccgcc gccgccaccg gcttggcatt
     6541 gatcatgtcc tgaggcatca ggccctcgga ctctgccagg ctcagccgct ccgtgaccgc
     6601 gcgctccact cgaatgaggc caacgcgaaa ctggttctcc gccatctcgc ccacggaccg
     6661 gacccgtcgg ttgccgagat ggtcgatgtc gtccacagac cctctaccgt tacggatgtc
     6721 gatgagggta cggagcacgt cgatgatgtc ctcgttggtg aggatgcctt cgccggtgat
     6781 gtcttcacgg ccgagccgcc ggttgaactt catgcgcccg acgcccgaga gatcgtaacg
     6841 ctcggcggag aagaacaggt tcgtgaacag gttttccgcc gcctccttgg taggcggctc
     6901 gccgggacgc atcattcggt agatctccac cagcgcctcg aggcgattgc tggtcggatc
     6961 tatcctgagc gtttccgata tgtaaggacc acaatcgaga tcgttggtgt agatggtctc
     7021 gatgtcggaa acgccggttt cgagaagctt gtcgagcacc tcgtcggtga tgatcgagtt
     7081 gcaagcaacg acgatctccc cggtgctctc gtccacaacg tccgcggcga tcacccggtc
     7141 gagcatgtac tcgcgcggca cgtccaggcg cttgagaccg ctgtcctcga gcaaccggac
     7201 gtgtctcgcc gtgatgcgcc ggccgagctc gacgatgacg ttgccctgcc ggtcggtgat
     7261 gtcgaaggtc gcgacgtcgc cgcgcatcct ctcagggatc aggtcgaccg agaagccgtc
     7321 tttcttgacg tggaaaatgt tcttgtcgaa gaacataccg aggatctcct cggcgctgaa
     7381 gtcgagggcc cgcaggatga tcgtcgccgg cagcttgcgg cggcggtcta tgcggacgaa
     7441 cacgcaatcc ttcggatcga actcgaagtc gagccacgaa ccgcggtagg ggatgacccg
     7501 ggcagagtag agcagcttcc ctgaagagtg ggtcttgccg cgatcgtggt cgaagaaaac
     7561 tcctggagag cgatggagct gggataccac cacccgctcg gtaccgttga tcacgaacgt
     7621 gccgttgtcg gtcatgagcg gaatctcgcc catgtagact tcctgctcct tgacgtcctt
     7681 gaccgccttg ttcgacgact ccttgtcgta aatgatcaag cgcactttga cccgcagcgg
     7741 cgcagcgtat gtcgtacccc tgagcacgca ctccttgacg tcgaaagccg gctggccgag
     7801 gcggtagtcg acatactcca aagccgcgtt gcccgaataa ctgacgatcg ggaagactga
     7861 tcgaaacgca gcgtgcagac cgatgtcgtc acggccttca gcgggcgccg ccagctgaag
     7921 gaacctgtca tacgaatcca cctggatgga gagcagatag ggcatctcca tcatttccga
     7981 cagcttgccg aagtctttgc ggatgcgttt tttctccgtg aagctatacg ccattcgaat
     8041 ctcctgcgtc agtacgcaaa ttcagtttgt ttcgtcgaac caatgccgac aggccggaag
     8101 cccctgggca gaaccatgtc tgccgcgggc gaccagccca tccttgcatg cccggcaatc
     8161 cgagaggtca accgcaaaac cgcggctact tgagctcaac agacgcgcca gcttcttcca
     8221 actgcttctt gatttcttcc gcctcgtcct tgccggcgcc ttccttgatc ggggagggcg
     8281 cttcgtcgac cagtgccttg gcttccttga gaccgaggcc cgtgatggcc cgaacggcct
     8341 tgatcacgtt gaccttcttc tctccggcgc cagagagaat gacgtcgaac tcgtccttct
     8401 cttcggcggc gccgtcgcca ccttccgccg cggcagcgac cgggcctacc gctacggcag
     8461 cggcggcgga cacgccgaac ttctcttcca tcgcctgcac gagctcgaca acttccatca
     8521 cgctcatgtc tgcaatcgca tttaaaatgt cgtctttaga caatgccatc tctgaatttc
     8581 tcctgaccca atttcctgga ttcgttcgcc aaggttaacc ttcagcggcg gcgtcgccgc
     8641 cagcctgttt ctgatccctg atcgcagcca ctgtgcgcac cagcttgccg ggaacctcat
     8701 tgaaggttcg ggcaagcttc gtgatcggcg cctgcattac gctcatcagg agcgccagcg
     8761 cttcatcacg ggtgggcaac ttggcaacgc gatcgatgtc ctccggcccg taaacctgac
     8821 cgccgatgga caacgccttg accttcaggg cgtcatattc cttcgcgcag tccttgatca
     8881 gacgggcggc cgatccagga tcttcctgcg agaacgcaac catcgtcggc ccgacgaacg
     8941 cttcgttgag gcactcatac tccgtgcctt cgatcgcccg tttggccagc gtgttgcgaa
     9001 tgaccttcaa gtagacgccc gagctcctgg ccttcgccct gagctcggtc atctccgaaa
     9061 cggtcagccc gcgatagtcg ctgagcacag cggccaacgc ggtcgtcgca gcctggttca
     9121 cctcggcaac aatcgcttgc ttgtcttcaa gtctaagtgc catcgtttct cctcggatat
     9181 cgccgatatc caccaacgcg gcgctaagcg ccgcggtcat tttcacggcg acccgagtcg
     9241 acgagacggg cattgcgggt tagagcaccc atgcctatgt cgttgaatca ggctctaccg
     9301 tctgcgtagg aaattaaggc tgaggggcat ctccttgcgg agcagccgtt ctcgaccacc
     9361 tacggttttt gacggaaccc ggcgcagccg tagcaagctt cctgcgaacc ccgcgtgcag
     9421 cagcaccgga ccttcaaaat ccattcgtat caactgttca aacctcgagc gacgcctggt
     9481 cgatggctac gccgggaccc attgtcgtcg agagcgtgat cttcttgaga tacacacctt
     9541 tagcggacgc cggcttcgcc ttgttcaggt cctggaccag cgccttgacg ttttctctga
     9601 tgtgttccgc ttcgaaacca acctgaccga cgctgccgtg aatgattccg gccttatcgg
     9661 tgcggaactg aacctggcca gccttcgcgt tggcaacggc agtggccacg tccggcgtta
     9721 ccgtgcccgt cttcgggttg ggcatcatgc ccctcggccc cagaatcttg cccagctggc
     9781 cgaccacccg catggcgtcg ggggtggcga tgacgacgtc gaaattgaga tcgcccgcct
     9841 tcactttctc ggccaggtcc tcgaagccga cgatgtccgc tccggccgcc gtcgcagcat
     9901 cggcactctc cccttgggca aagaccgcaa cccgcacttc ctttccggtt ccgtggggca
     9961 gggtcgtagc accccgtacg acctgatccg actttcgcgg atccactccg aggttcacgg
    10021 agatgtcgaa cgattccttg aacttggcct tggccaggct ccccagcaac tccaccgcct
    10081 cctcgatcgg gtagctcttc tcccggtcga ccttctcggc gattgcacgc attcgtttgg
    10141 tcattttggc catcagttga cgccctccac ctcgattccc atgcttcgcg ccgttcccgc
    10201 aatcgtgcgc acggcggcat ccatatccgc agcggtcagg tcaggcatct tggactgcgc
    10261 gatctcttcg agctgggagc ggttgacctt cccgactttc tgggtgttgg ggttcgggct
    10321 gcctttggcg agcttcgccg ctttgcgcag cagcactgcg gccggcgggg ttttcgtgac
    10381 gaaggtgaag ctgcgatccg cgtagacggt aatgaccacc ggggtcggca gaccctgctc
    10441 gagactctgc gtctgggcat tgaacgcttt gcagaagtcc atgatgttca cgccgtgctg
    10501 acccagggcg ggccccaccg gcgggctcgg attagcctgc cccgccgcga cctgcagctt
    10561 gatgtaggcg tcaatttttt tagccatcga tgatgtctcc tatgggttca gacgtgcgcg
    10621 gaccggttcg ctaccgacag ctacactccc cgttaccggg ctttagccct tctccacctg
    10681 cccgaaatcg agctccactg gagtcgaacg cccgaaaatc gtcaccgcca cctgaaggcg
    10741 gctcttctcg taattgacct cctcaacgac gccgttgaaa tcgttgaacg gcccgtcgat
    10801 gacccgaacg atctcgccgg gctcgaatat ggtcttcggc gtcgccttgt cgctgcccgc
    10861 cgccacgcga tccagaatcg ccgccgcctc ggcttccgag agcggagcgg gttggtccgc
    10921 tttcccgccg atgaagccca tgactcgcgg cgtctccttc acgagatgcc aggtgtcgtc
    10981 gttgagctcc atctccacga gaacgtagcc cgggaaaaac ttgcgctcgc tgcgacgctt
    11041 ctggccgccg cgcatctcca ccacctcttc ggtcggaacg agaatctcgc cgaagtgatc
    11101 gccgagcgac gaaagctcga cgcgttcctt caaggcccga gcgacgttct tctcgaaccc
    11161 cgagtaggcg tgcacgacat accatttctt cgacatgatc cgcctaccct atgaccgatg
    11221 aaaccagcca gctcaaaccg gagtcgagca gccacaagag cagagcgaaa acgagcacca
    11281 gcaccagaac gatcatcgtc gtctgcagca cctcgtcccg ggtcggccag accacgcgcc
    11341 gaatctcgct gcgcgcctct ttcatcaggc tccacgtcgt gcggcctttc tcggtctgaa
    11401 cagcaatcat cacggcggcc accgccatgg ctatcagccc gccgacacga agcagcagcg
    11461 attcgtcctg gtaataccag ttgccgaaga cgccgccgcc aaccaacacc gcaacgacca
    11521 accacttaag ccagtccatg gcagattgtc cggttgttgc tgacccgctg ctcaaatcaa
    11581 atgtccctgc tcatctggca ggccaggagg gaatcgaacc cccaacctgc ggttttggag
//
LOCUS       DBNHLM010000232         4133 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1231122, whole genome shotgun sequence.
ACCESSION   DBNHLM010000232 DBNHLM010000000
VERSION     DBNHLM010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1231122"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /locus_tag="AC16QF_10715"
     CDS             <1..1355
                     /locus_tag="AC16QF_10715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="spinster family MFS transporter"
                     /protein_id="HIR6449071.1"
                     /translation="LPAVSESLPETRAGGDARPATLGYAWYVVGVLLLAQTFSFLDRM
                     IMGLLVGPIRETFQISDTQYSLLAGLAFSLFYAVMGLPLARIADSGNRRNLISLGIAV
                     WSMMTAVCGLARGFWTLFIARVGVGVGEATLAPGAYSMITDYFPRGLLARALSVYMIG
                     VTLGSGFAYMLGGAVVAYVEHMPQIVLPLLGEVYGWQLTFFIVGLPGILVSVLMVLTV
                     REPKRHGLTDGSQASIPLPEVVSFLWQRRRAYGGHIFGISIFIMVVYALNLWGPSYLI
                     RTFGYSRPEAGWIFGVVMIVAGTAGLLLAGTLADAWMKRGTRDAYVRTILLSIVCITP
                     CAVALGFIEDPALGILFIGLAVFFSAFQGGIAGGTLQLMTPNRMRGQVTAVYQLVANL
                     IGLGLGPTVVAATTDYVFGYDEAIGKSIALSAAVLCPVGGLLLWRSLSAIRGQLEEQM
                     "
     gene            complement(1408..2448)
                     /locus_tag="AC16QF_10720"
     CDS             complement(1408..2448)
                     /locus_tag="AC16QF_10720"
                     /inference="COORDINATES: protein motif:HMM:NF013847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449072.1"
                     /translation="MIELPLLPTSLVGSYAQPAWLIDRDRLGQRLPPRVLLDALWKVD
                     ALSLEEAQNDATVIALEDQHRAGVDIVTDGEMRRESYSNRFANALEGVDLDNPGAAVD
                     RTGKTVPVPRVVGEIRRREPVEAPFIAFLKRHTDRPIKITLPGPFTMAQQAQNDYYAY
                     EGSLAMAYATAVNAELKDLFAAGVDIVQLDEPYVQARPEAAREYAVASIDRALEGASG
                     PTVLHVCFGYGQHVADKPAGYAFLDELNDCRADEISIECAQPRLKMDLVQGLPNKKVH
                     VGVLDLRDQTPETPETVAERIRSALEFLPAERMVVAPDCGMKYLPREVAFAKLANMVR
                     GRDLVRADLAGP"
     gene            complement(2516..>4133)
                     /locus_tag="AC16QF_10725"
     CDS             complement(2516..>4133)
                     /locus_tag="AC16QF_10725"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6449073.1"
                     /translation="VHTAYSLDAYAFGAIAAPKEAYAFGRGQPLRLANGEMVAIDRPL
                     DFMAVTDHAETYDVMYICTDPLYRDDAYCRAIRSGPEPGNARTVFNDYLLPLVSDQPP
                     QMAAVCDEDGVDCRAASIGQWRRAQRAANAANDPCTFTALIGYEWTASPGGHHWHRNV
                     IFRSDRVPDRAADYVHFPEVTALWRELDTHCRSEDGCEVLTIPHNINWADGGRTFDIE
                     HASEADLAARARFERLAEIHQEKGNSECLPAHRDDPGSDCAFELLTDNAAKARLTGPE
                     DISPEDAWRRMRSTYYRGLLARGLAAYASSESGLNPFMLGAIGSTDTHFGTPGLVSEA
                     GYPGGIASLWLDGEERLTLPNYNPGGLVAVWAQENTRAAIFDSLQARSAYATSGTRIK
                     LRFGATEHDACENSKVNYDTFMGETLTTGLPSDNTAGPVFTVEAGRDEAPLGTVQIIK
                     GELREDGVVETVHTVADFPDGRDAVCISWQDEAFTAAAPAYWYARVLELPAPRWTKLL
                     CEATGSCDRFPDADRNIRERAWSSPIWHLP"
BASE COUNT          608 a         1328 c         1402 g          795 t
ORIGIN      
        1 gccttccagc ggtgagcgaa tcgctcccgg agacccgggc gggtggtgac gcccgcccgg
       61 ccaccctcgg ttacgcctgg tacgtcgtcg gcgttctgct gcttgcgcag accttctcct
      121 ttctcgaccg gatgatcatg ggtctcctcg tggggcccat ccgggagacc ttccagatca
      181 gtgacactca gtacagcctg ctggcaggcc tggccttctc gctcttctac gccgtgatgg
      241 ggctgccgct ggcgcgcatc gcggactccg gcaaccgccg caacctcatc agcctgggca
      301 tcgcggtgtg gagcatgatg accgcagtct gcggcctggc ccgaggcttc tggacgctgt
      361 tcatagcccg ggtaggcgtc ggcgtgggcg aagcgacgct agcgccgggc gcgtactcca
      421 tgatcaccga ctactttccc agaggccttc ttgcccgggc gctatccgtg tacatgatcg
      481 gggtgacgtt gggctccggt ttcgcctaca tgcttggcgg cgcggtcgtg gcctacgttg
      541 agcacatgcc ccagatcgtc ctgcccctgc tcggagaggt gtacggctgg cagctcacct
      601 tcttcatcgt cggtctgccg ggcattctgg tgtccgttct catggtgctg accgtgcgtg
      661 agccgaagcg ccacggcctg accgacggct cgcaggcctc catacccctt cccgaggtgg
      721 tcagctttct ctggcagagg cgcagggcct atggcggtca catcttcggc atatccatct
      781 tcatcatggt cgtctacgcg ctcaatctct gggggcccag ctacctgatt cgaaccttcg
      841 gttacagccg cccggaagcc ggctggatct tcggtgtcgt gatgatcgtg gcgggaaccg
      901 ccgggcttct gctggccggc accctggcgg atgcctggat gaagcgtggc acccgggatg
      961 cctacgtgcg gacgatcctt ctcagcatcg tctgcatcac cccgtgcgcg gttgcgctcg
     1021 gattcattga agatcctgcg ctgggaattc tcttcatcgg gctggccgtt ttcttttcag
     1081 cctttcaggg cggaatcgcg ggcggcacgc tgcagctcat gacgccgaac cggatgcgcg
     1141 gccaggtcac cgccgtttac cagcttgttg ccaacctcat cgggctgggc ctgggaccaa
     1201 cggtggtggc tgccaccacc gactacgtgt tcggctacga cgaggccatc ggcaaatcca
     1261 tcgcgctgtc ggccgccgtc ctctgtccgg tcggcgggct gctgctctgg cgcagcctgt
     1321 cagccattcg cgggcagctg gaggagcaga tgtgatcccg cccggatgac ggcggcgtag
     1381 cgcttcccgg actcggaaag gagccgttca gggtccggcc aggtcggccc taacgaggtc
     1441 gcggcctctc accatgttgg ccagcttcgc gaaggcgacc tctctgggca ggtacttcat
     1501 gccgcagtcg ggagcgacca ccatgcgctc ggcgggcagg aactccagtg ccgaccggat
     1561 gcgttcggcc acggtttccg gcgtctcggg agtctggtcg cgcagatcca gcacgccgac
     1621 gtgcaccttc ttgtttggca ggccctggac cagatccatc ttgagtcgcg gctgcgcgca
     1681 ctcgatggag atctcgtccg cccggcagtc attgagctcg tcgaggaacg cgtagccggc
     1741 cggcttgtcc gcgacgtgct gtccgtagcc aaagcagacg tgcagcacgg tcggtccgct
     1801 ggcgccttcc agggcccgat cgatggacgc caccgcgtac tcccgggcgg cttccgggcg
     1861 cgcctgcacg tagggctcgt ccagctggac gatgtccacg cccgcagcaa agagatcctt
     1921 gagctccgcg ttgacggccg ttgcgtacgc catggccagc gatccttcat atgcgtagta
     1981 gtcgttctgc gcctgctgcg ccatggtgaa cgggcccggc agggtgatct tgatgggccg
     2041 atcagtgtgc cgcttgagaa aagcgatgaa cggggcctcc acgggctcgc ggcgcctgat
     2101 ctcgccgacc acgcgcggca ccggcacggt ctttcccgtc cggtccacgg ctgcgccggg
     2161 attgtcgagg tccacgccct ccagggcgtt ggcgaaccgg ttcgagtagc tttcccgccg
     2221 catctcgccg tcggtgacga tgtccacgcc ggcgcgatgc tgatcctcca gggcgatgac
     2281 cgtggcgtcg ttctgggcct cttcgaggga aagggcgtcc accttccaca gggcgtcgag
     2341 caggactctg ggcggcagcc gctggccgag ccgatcgcga tctatgagcc acgcgggctg
     2401 tgcgtagctg ccgaccagcg aggtcggtag aaggggtagt tcgatcatca gggctcctcg
     2461 gatctccgtt gcgtgttgtc tcgcgtattc ggaatgacgg cgctttcctc gggtttcatg
     2521 gcaggtgcca gatgggtgag gaccatgccc gctcccggat gttccgatcc gcatcgggga
     2581 agcggtcgca cgacccggta gcttcgcaga gcagcttggt ccagcgtggc gcgggcaact
     2641 ccaggacgcg cgcgtaccag taggcgggcg cggcggcggt aaatgcctcg tcctgccacg
     2701 aaatgcagac cgcgtcgcgg ccgtccggaa agtccgccac ggtgtggacg gtttccacca
     2761 caccatcctc gcgaagctcg cccttgatga tctgcacggt tcccagcggc gcctcgtcgc
     2821 ggccggcctc aacggtgaac acgggcccgg cggtattgtc ggacgggagc ccggtcgtca
     2881 gggtctcgcc catgaacgta tcgtagttca ccttggaatt ctcgcaggcg tcgtgctcgg
     2941 tcgcgccgaa gcgcagcttg atcctagtgc cgctggtggc gtaggcgctg cgcgcctgca
     3001 gggaatcgaa aatggcggcg cgggtattct cctgtgccca cacggccacc agcccgcccg
     3061 gattgtagtt cggcagcgtc aggcgttcct cgccgtccag ccacagggag gcgatgccgc
     3121 caggatagcc ggcctcactc accaggcccg gggtgccgaa gtgggtgtcg gtagagccta
     3181 tggcgcccag catgaagggg ttgagtccgg actcgctgct cgcgtaggcc gccaggcccc
     3241 gggcgagcag gccgcgatag taggtcgagc gcatgcgtcg ccaggcgtct tcgggggaga
     3301 tgtcctcggg gccggtcagt cgcgccttgg cggcgttgtc ggtcaggagc tcgaacgcgc
     3361 agtcgctgcc tggatcatcc cggtgggcgg gcagacactc gctgttgccc ttctcctggt
     3421 gaatttccgc cagccgttcg aaacgcgccc gggccgccag atcggcttcg ctcgcgtgct
     3481 cgatgtcgaa agtgcggcca ccgtcggccc aattgatgtt gtggggaatg gtgagcacct
     3541 cgcagccgtc ctcgcttcga cagtgggtgt ccaattcccg ccacagggcg gtgacctcgg
     3601 gaaaatgcac gtagtcggcg gcgcgatcgg ggacccggtc actgcggaag atcacgttgc
     3661 ggtgccaatg gtggccgccg ggtgaggcgg tccattcgta gccgatcagg gcggtgaaag
     3721 tgcagggatc gttggccgcg ttggccgcgc gctgcgcgcg tcgccactga ccgatgctgg
     3781 cagcgcggca atcgacgccg tcttcgtcgc agactgcggc catttgcgga ggctggtcgc
     3841 tgaccagcgg cagaaggtag tcgttgaaga cggttctggc gttgccgggt tcggggccgc
     3901 tgcgaatggc ccgacagtag gcgtcatccc ggtagagggg atcggtgcag atgtacatca
     3961 catcataggt ctctgcgtga tcggtgactg ccatgaagtc cagtggacga tcgatggcca
     4021 ccatctcccc gttggccaga cgcagaggct gaccgcggcc gaacgcgtag gcctctttgg
     4081 gcgctgcgat ggcgccaaat gcgtaggcgt cgagggagta ggccgtgtga acg
//
LOCUS       DBNHLM010000233         4442 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_750703, whole genome shotgun sequence.
ACCESSION   DBNHLM010000233 DBNHLM010000000
VERSION     DBNHLM010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_750703"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /locus_tag="AC16QF_10730"
     CDS             complement(<1..1416)
                     /locus_tag="AC16QF_10730"
                     /inference="COORDINATES: protein motif:HMM:NF025114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6449074.1"
                     /translation="MPDNDDRISEPAADGELDVAIVGAGFSGLYMLHRVRDVLKLKTR
                     VFEAADGVGGTWYWNRYPGARCDSESYFYCYSFSKDLEQEWTWTSKFPEQPEILSYLN
                     HVADRFDLRRDIQLNTRVTGALFEDRSNRWIISTDTGETVSARFLISAVGCLSTRNLP
                     DIPGIESFAGEHYHTGNWPHEGVDFRGKRVGLIGTGSTGIQAAPVIAEEAEHLYVFQR
                     TPNFSVPARNTPLTAERTAEIKANYDEIRRISRTSGFGFPYETPEHAAMEVTPEERQS
                     VYEQRWHEGGTSFGFRSFSDILSDQAANDSASDFIRSKIRETVKDPEVAELLIPHDHP
                     YGTKRPPLDTNYFETYNRDNVTLVDVRSAPIKEITPRGVRTARDEYELDVIVFATGFD
                     AMTGALLAMNIEGSGGLRLSDKWANGPVTYLGLQVAGFPNLFTITGPGSPSVLANMPV
                     AIEQHVEWVSDCLEHLYANGIS"
     gene            1540..3558
                     /locus_tag="AC16QF_10735"
     CDS             1540..3558
                     /locus_tag="AC16QF_10735"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6449075.1"
                     /translation="MSGNDFVPKQAAAGLLLLAVVGCGSEEAPPADAGREQALPAGTN
                     SGHLVAYSEVREPCANADPLRIPLFGDVHVHTSFSFDAAANSVGTTPADAHKFALGEP
                     IPFFPVDASGTPTGTIRIDRPLDFLAVTDHGEFLGERSLCRDPRSPKYSSAFCEEYRT
                     GERHGMRMLGRVITSETPRRVPELCGDDGALCLEYAEGPWRRIVEAAEAAYDRSPGCT
                     FTSFVGYEYTGTPGTSNYHRNVIFRNASVPPRPVSYIDAPVDSLLWNALDAACGAVDG
                     CDYLTIPHNSNLANGRMGPYRGIELTEANRRAYAEQRQRREPVMEMFQHKGASECING
                     LTTVLGEPDELCDVEAVRVIGRAETYQTARMVDGELVLGETTEVTQECAPGESSSNGM
                     LGAGCVSATDYMRSGLLVGLAEEQEIGVNPIKLGMIAATDTHTSTPGAVAEGDWRGHV
                     TGEATPAERLEPGLLTSGIDGNPGGLAGVWAVENSRDAIFDAMLRREVFGTSGPRIVP
                     RFFAGWGYDAEMCGSADLVARGYGGGVPMGGDLPRGAPGDRPAFIAWAARDPAPSAQP
                     LESLQLVKGWIDAEGQARNRVIPIAGGEGQVARDTLCAVYRDDDFDPAVPAYYYLRVV
                     EPPTPRWSTFDCQRLPESDRPAVCSDGSYPQTIREMAWTSPIWYRPEG"
     gene            3566..3991
                     /locus_tag="AC16QF_10740"
     CDS             3566..3991
                     /locus_tag="AC16QF_10740"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043023 - ribosomal large subunit binding
                     [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA];
                     GO_process: GO:0045947 - negative regulation of
                     translational initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HIR6449076.1"
                     /translation="MPRVERSIEIAAPVSRVQQIIVDFERYPEFVPFMERAELLGIEG
                     ERRRVRFTLNLVARISYTLDLVPGTDSGLTWTLVDGPFVRNNGSWRLKATNDDGTLAT
                     YTADVELKGFLPGFLERRLVAESLPATLRAFRDRAESDA"
     gene            complement(4052..>4442)
                     /locus_tag="AC16QF_10745"
     CDS             complement(4052..>4442)
                     /locus_tag="AC16QF_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6449077.1"
                     /translation="FHYTLFPNFAVSIWADGFHFLRARPHPTDPERCVFDNWWYASQP
                     EGETELVSTMIGPVDRDAVVEHEVFDYGEQTMGMTIDQDMAVFIRQQKGFRSRGFKGV
                     YLSKQESRVRRYHELIDDYIEGRLPKR"
BASE COUNT          724 a         1419 c         1502 g          797 t
ORIGIN      
        1 tgagatgccg ttcgcgtaga gatgctcgag gcagtcagac acccactcga cgtgctgctc
       61 gatggccacc ggcatgttgg ccagaaccga agggctgccg ggcccggtga tggtgaacag
      121 gttcggaaat ccggccacct gcagacccag gtaggtgacc ggcccgttgg cccacttgtc
      181 agaaagacgc agccccccgg atccctcgat gttcattgcg aggagtgccc cggtcatcgc
      241 gtcgaagccg gtggcgaaga cgatgacgtc gagctcgtac tcatcccgtg cggtcctgac
      301 cccgcgggga gtgatctcct tgatgggggc cgatcggacg tcgaccaggg tgacgttgtc
      361 gcggttgtag gtctcgaagt agttcgtatc cagtggtgga cgtttggtgc cgtaggggtg
      421 atcgtgggga atgaggagct ccgcgacctc agggtccttc acggtctcac ggatcttgct
      481 ccggatgaag tccgacgcgc tgtcgttggc ggcctggtca gacagaatgt cgctgaacga
      541 ccgaaagccg aagctggtac ctccctcgtg ccaccgctgc tcgtagacgg actgacgctc
      601 ctcgggtgta acctccatgg ctgcgtgttc cggcgtctcg taaggaaagc cgaaacccga
      661 agtgcgcgag atccgtcgga tctcgtcgta gttggccttg atctctgccg ttcgctcggc
      721 cgtcagcggg gtgttgcggg cggggacgct gaaattcggc gtgcgctgaa agacgtagag
      781 gtgctcggct tcctcggcga tcaccggtgc cgcctggatt ccggtggagc ccgtgccgat
      841 gaggcccacg cgctttcccc ggaagtcgac gccttcatga ggccagttgc ccgtgtggta
      901 gtgctcgccg gcaaagctct cgatgccggg aatatccggc aggttccggg tcgagaggca
      961 acccacagcg ctgatcagaa agcgggcgga cacggtctcg ccggtgtcgg tggagattat
     1021 ccagcggttg ctccgatctt cgaaaagggc gccggtgact cgtgtgttga gctggatgtc
     1081 acggcgcaga tcgaaccggt ctgccacgtg attgaggtag ctgagaattt ccggttgctc
     1141 ggggaacttg ctcgtccacg tccactcctg ctcgagatcc ttggagaacg agtagcagta
     1201 gaagtagctt tccgaatcgc agcgggcacc tgggtaccgg ttccagtacc atgtgccgcc
     1261 gaccccgtcc gccgcctcga agacgcgcgt cttcagcttc agcacgtcgc gcacccggtg
     1321 cagcatgtag aggccggaaa agcccgcgcc gacgatggca acgtcgagct cgccgtccgc
     1381 cgctggctcg gaaatcctgt cgtcgttgtc tggcatgctt ctctccctcc gaccttcctt
     1441 ctagtttgcc ggtttcgccg tgacctgcaa gtcgtgaccg gcgcggccga tcggctccat
     1501 gctaaggttg acggtcgcga ggatcgggag ggatttgacg tgagcggaaa cgatttcgtt
     1561 cccaagcagg cggcagccgg cttgctgctg ctcgccgtcg tcggctgcgg aagtgaagag
     1621 gcgcccccgg cggacgccgg gcgggaacag gcgttgcctg ccggcacgaa ctcggggcat
     1681 ctcgttgcct acagcgaagt gcgcgagcct tgcgccaacg ccgatccgct gcgcataccc
     1741 ctgttcggcg acgttcacgt tcacaccagc ttctcgttcg acgccgcggc gaacagcgta
     1801 ggtaccacgc ccgcggatgc gcacaagttc gcgcttggcg agcccatacc cttctttccg
     1861 gtagacgcga gcggcacgcc gacgggcacc atccgcatcg accggccgct ggatttcctc
     1921 gcggtgaccg accacgggga gttcctgggc gagcggtctc tgtgccggga cccgcgctcg
     1981 ccgaagtact cgtccgcttt ctgcgaggag taccggactg gcgagcgaca cggcatgcgc
     2041 atgctgggca gggtaatcac ctcggagacg ccgcgacggg tgcccgagct gtgtggagac
     2101 gacggcgcgc tgtgtctgga atacgctgag ggtccgtggc gccggatcgt cgaggccgct
     2161 gaggccgctt acgatcgctc gcccggctgc acgttcacca gcttcgtcgg ctacgagtac
     2221 acgggcacgc ctggcacctc gaactaccac cgcaacgtaa tcttccgcaa tgccagcgtc
     2281 ccgccgcggc cggtgtctta catcgatgcg cccgtggaca gtctgctctg gaacgcgctg
     2341 gatgcggcct gcggtgccgt cgatggttgt gactatctga ccattccgca caactcgaac
     2401 ctggccaacg gacggatggg cccttatcgg ggcatcgagc tcaccgaggc gaatcggcgt
     2461 gcttacgcgg agcagcgtca gcgccgggag cccgtcatgg agatgttcca gcacaagggg
     2521 gcatcggagt gcatcaacgg gctgaccacc gtgctcggcg agccggacga gctgtgcgat
     2581 gtggaggccg tccgggtgat cggccgcgcg gagacgtacc agacggcgcg gatggtcgac
     2641 ggtgagctgg tgctggggga gaccaccgag gtcacccagg aatgcgcgcc cggggagtct
     2701 tccagcaacg gcatgcttgg cgccggctgc gtgtctgcca cggattacat gcgctccggg
     2761 ctcctggtag gcctggccga ggagcaggaa atcggggtca atcccataaa gctcggcatg
     2821 atcgccgcca cggacaccca cacctccact cccggggccg tggcggaagg cgactggcgc
     2881 ggccacgtga ccggcgaagc gacgccggcc gagcgcctcg agccggggct gctgaccagc
     2941 gggatcgacg gcaatcctgg cggcctggca ggcgtctggg ccgtggaaaa ttcgcgcgat
     3001 gccatattcg acgccatgct ccggcgggag gtgttcggca cctccgggcc ccgcatcgtg
     3061 ccgcgctttt ttgcgggctg gggctacgac gccgagatgt gcggcagcgc cgacctggtg
     3121 gctcgaggct atgggggcgg ggtgcccatg ggcggcgatc tcccccgggg tgccccgggt
     3181 gaccggcccg ctttcatcgc ctgggcggcg cgggatccgg cgccctcggc ccagccgctc
     3241 gagtcgctgc agctggtcaa aggatggatc gacgccgagg gtcaggcgcg caaccgggtc
     3301 attcccattg ctggcggaga ggggcaggta gcgcgggaca ccctttgcgc cgtttatcgc
     3361 gatgacgact tcgatcccgc tgtgcccgcg tattactacc tgcgcgtggt ggagccgccg
     3421 acgccgcgtt ggagcacctt cgactgtcag cgtctgcctg aatccgaccg gccggccgtg
     3481 tgcagcgacg gctcgtatcc ccagacgatc cgggaaatgg cctggacgtc tcccatctgg
     3541 taccggcccg agggctgagc ggcagatgcc cagggtcgag cgcagcatcg agatcgcggc
     3601 gcctgtatcg cgcgtgcagc agatcatcgt cgacttcgaa cgctatccgg agttcgttcc
     3661 cttcatggaa cgcgcggagc tgctgggtat cgagggcgaa cgacgccgcg ttcgcttcac
     3721 gctgaatctg gtagcgcgga tcagctatac cctggacctc gtgcccggca ccgattcggg
     3781 cctgacatgg accctggtag acgggccctt cgtgcgcaac aacggcagct ggcgtcttaa
     3841 ggccacgaac gacgacggga ccctggcgac ctacaccgcc gacgtcgagc tcaagggttt
     3901 tctgccggga ttcttggaac gacggctcgt cgccgaaagc cttcccgcca ccctgcgcgc
     3961 gttccgcgac agagccgagt cagacgccta ggcagccggt cgccccacgg ctgccgcccc
     4021 cggagggttc ggcttcttca ggccgcctgg atcagcgctt gggcaggcgc ccctcgatgt
     4081 agtcgtcgat gagctcgtga tagcggcgga cccgactctc ctgcttggac aggtagacgc
     4141 ccttgaaacc ccgggaccgg aatcccttct gctggcggat gaacacggcc atgtcctggt
     4201 cgatggtcat gcccatggtc tgctcgccgt agtcgaacac ctcgtgctcc accacggcgt
     4261 ctcggtccac cgggccgatc atggtgctca cgagctccgt ctcgccctcg ggctggctgg
     4321 cgtaccacca gttgtcgaag acgcaccgtt ccggatcggt gggatggggc cgcgcccgca
     4381 gaaagtgaaa gccgtcggcc cagatcgaca ccgcgaagtt cggaaacagc gtgtagtgaa
     4441 ac
//
LOCUS       DBNHLM010000234         3443 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1050683, whole genome shotgun sequence.
ACCESSION   DBNHLM010000234 DBNHLM010000000
VERSION     DBNHLM010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1050683"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="AC16QF_10750"
     CDS             <1..497
                     /locus_tag="AC16QF_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449078.1"
                     /translation="RRVEALGIRIVNQYDSGEFRNMQMHPKDTGGSFFEIDQQLGDGA
                     HDPDGPWEPAGPDWQQGRQLDRVSGIAAAEIQADDPARVAACWSEIAEIPLTTTADGH
                     PALVLDNATVRFVPCTDGRPEGLGGIDVLATNAAAIMATARDQGLATGERQIYLCGMR
                     INLV"
     gene            494..1309
                     /locus_tag="AC16QF_10755"
     CDS             494..1309
                     /locus_tag="AC16QF_10755"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6449079.1"
                     /translation="MSGYTDIDVQIEDPACLIRLNRPDKLNAFTYHTLREIRQAIDAA
                     AADSRVVGIIITGNGRGFSAGLDAQVLAEVTSRGGSAADDSAGEDDLPGIFSYLLEVP
                     KPVIAAVNGVAAGGGLILALMSDLRIASRDASFITVFLKRGLVAEHGSSWLLPRLVGT
                     GRALDLLWMSDRIDAQTAADIGLVERLAEPDELIPSALDYVTRLAEGSAPKAIAATKQ
                     LVYRHLGMGYADALKEADRVQRRFVAAADAAEGARALLEKRSPRFERLGTTDD"
     gene            1325..2095
                     /locus_tag="AC16QF_10760"
     CDS             1325..2095
                     /locus_tag="AC16QF_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6449080.1"
                     /translation="MQTILFESSGGVGTLTLNRPDRLNGMTNQMLIETHDCLLEVAQR
                     DDVRVLVLTGAGRGFCPGADLKHYAAGEADVASQPRHFQVPVLLHEMPQITIAAINGP
                     CAGAGLGWACGCDLRIAARSANFNTAFLKVASAGDMGGPWSLPRLIGAAKARELYFLA
                     EKFGAEEALAMGLVSRVFEDEQFAEKAAAMAGALASSSPHALRTMKQNFLDAERLNFA
                     DYVELETGRHLEVMAHPDAREAFRAFVEKRPPRFESSE"
     gene            2132..2953
                     /locus_tag="AC16QF_10765"
     CDS             2132..2953
                     /locus_tag="AC16QF_10765"
                     /inference="COORDINATES: protein motif:HMM:NF006123.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6449081.1"
                     /translation="MSGDEQKSIFITGGAGGMGMATGRLFAQHGWFVGLFDLDESALE
                     LARAAFDDSQSLVRRLDVTSETDFADAMAAFSERTGGRMDVMFNNAGIAPGAWFDEMP
                     LDTIRQVIDINVMGVIIGIRAALPLLRATPGSLCMSTSSSVATYGHAMRAAYTASKFA
                     VKGLTEALSLEFERFGIRTADVLPGCIDTPMLRNALSARTGEPFDESLFETLPKEGAY
                     RLVPVSAIADAVWNAYHSDVIHWYVPEEVGEIDRLKGADFDAARAETRAFLFGEG"
     gene            complement(2968..>3443)
                     /locus_tag="AC16QF_10770"
     CDS             complement(2968..>3443)
                     /locus_tag="AC16QF_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6449082.1"
                     /translation="GLMEKMRDDQKLIRNFLEESLRFETPVLHLWRITTQDTDLGGAF
                     IPQGSMVAIGYASANRDEDVFQDSEVFDIERQKAGAHLAFGSGPHHCPGAALARQEMF
                     SAFTILLHRLDNIRLADPGDRFLHVPSSFLRGLEHLNLKFDVRDRERYLPGASAY"
BASE COUNT          593 a         1172 c         1146 g          532 t
ORIGIN      
        1 ggcgccgggt agaggcgctc ggcatccgca tcgtcaatca gtacgacagc ggcgagttcc
       61 gcaacatgca gatgcacccc aaggacaccg gaggatcctt cttcgagatc gaccagcagc
      121 tcggtgacgg ggctcacgac ccggacggcc cctgggagcc agcgggcccc gattggcagc
      181 agggccggca gctcgaccgc gtgagcggaa tcgccgcagc cgagatccag gcagacgacc
      241 ccgccagggt ggccgcgtgc tggtccgaga tagcggagat cccgctcacc accacggcgg
      301 acggccaccc ggcgctggtg ctggacaacg cgacggtacg cttcgtgccc tgcaccgacg
      361 gtcgcccgga gggccttggc ggcatcgacg tgctcgccac taacgccgca gccatcatgg
      421 ccaccgcacg cgatcagggc ctggccaccg gcgagcggca gatctacctg tgcggcatgc
      481 gcatcaacct agtatgagcg gctacacgga catcgatgtc cagatcgagg accccgcctg
      541 cctgatccgg ctgaaccgcc cggacaagct gaacgctttc acctaccaca cgctccggga
      601 gatccgccag gccattgatg ccgcggcagc cgattcccgg gtggtcggca tcatcattac
      661 cggcaacggc cgcgggttct ccgccggcct cgacgcccag gtgctggcag aggtcacctc
      721 gagaggcggg agcgcggccg acgattccgc gggtgaagac gacctgccgg gaattttcag
      781 ctacctgctg gaagtgccca aacccgtcat cgccgccgtg aacggcgtgg cggccggcgg
      841 cgggctcatt ctggcgctga tgagcgacct gcggatcgct tccagggacg caagcttcat
      901 caccgtcttt ctgaaacgcg ggctcgtggc cgagcacggg tcgagctggc tgttgccgcg
      961 cctcgtcggc accggccgcg cgctggacct cctctggatg agcgaccgga tcgatgcgca
     1021 gacggcggcc gacatcggcc tggtggagag gctcgcggaa cccgacgagc tgattccaag
     1081 cgccctcgat tacgtcacgc gcctggcgga gggctcggcg cccaaggcca ttgccgcaac
     1141 caagcaactc gtctaccggc acctgggcat gggctatgcc gacgcgctga aggaagccga
     1201 ccgggtgcag cgccgcttcg tggcggccgc agacgccgcc gagggcgcgc gcgccctgct
     1261 cgagaagcgc tctccccgct tcgaacgcct gggcacgaca gacgactgaa cggagccgaa
     1321 gcccatgcag accatcctct tcgaatcatc gggcggcgtg ggcacgctga ccctgaaccg
     1381 cccggatcgg ctgaacggca tgaccaacca gatgctgatc gagacccacg actgcctcct
     1441 cgaagtggcg caacgggacg acgtgcgcgt gctggtgctc accggagcgg gccggggttt
     1501 ctgccccggg gcggacctga agcactacgc tgccggcgaa gcggacgtgg cttcccagcc
     1561 acgccacttc caggttcccg tgctgctgca cgagatgccc cagatcacca tcgccgcgat
     1621 caatggcccc tgcgccggcg ccggtctcgg ttgggcctgc ggctgcgacc tgcgcatcgc
     1681 cgcccgctcc gccaacttca acactgcgtt cctcaaggtg gcgtccgccg gcgacatggg
     1741 cgggccctgg tccctgcccc gtctcatcgg cgccgcgaaa gcgcgggagc tgtatttcct
     1801 ggcagagaag ttcggggccg aggaggctct ggcgatgggc ctggtgagcc gggtcttcga
     1861 ggacgagcaa ttcgccgaga aagcagctgc catggccggg gcgctggcga gctcgtcccc
     1921 ccacgcgctg cgaaccatga agcagaactt tctcgacgcc gagcgcctga atttcgccga
     1981 ctacgtggag ctcgaaaccg gccgccatct ggaggtcatg gcgcacccgg acgcccggga
     2041 ggcgtttcgg gctttcgtcg agaagcgccc gccgcgtttc gagtccagcg agtaatccga
     2101 ccaagcacaa accattacga ggagacgcaa cgtgagcggg gacgagcaga aatcgatttt
     2161 cattaccggc ggggccggcg gcatgggcat ggcaaccgga cgcctcttcg cccagcatgg
     2221 ctggttcgtc ggcctcttcg acctcgacga aagcgccctg gagcttgcgc gggcggcttt
     2281 cgacgactcg cagagcctcg tccggcggct cgacgtcacc agcgagaccg acttcgccga
     2341 tgcgatggcc gcgttctccg agcgcacggg ggggcgcatg gacgtgatgt tcaacaacgc
     2401 agggatagcg ccgggggcgt ggttcgacga gatgccgctg gataccattc gtcaggtcat
     2461 cgacatcaac gtcatggggg tgatcatcgg catccgcgcc gccctgcccc tgctgcgagc
     2521 cacgccgggc tcgctgtgca tgagcacgtc ctcctcggtg gcaacttacg gacacgccat
     2581 gcgcgccgcc tacacggcct ccaagttcgc ggtgaagggc ctgaccgaag cgctcagcct
     2641 ggagttcgaa cgcttcggca tccgcaccgc cgacgtgctg ccaggctgca tcgacacgcc
     2701 catgctgcgc aatgccctgt cggcgcgaac gggcgagccc ttcgacgagt ccctgttcga
     2761 gaccctcccc aaggagggcg cctaccggct cgtgccggtt tccgccatcg cggacgccgt
     2821 ctggaacgcc taccactccg acgtgatcca ctggtacgtg cccgaggagg tgggcgagat
     2881 tgatcgcctc aagggcgccg acttcgacgc cgcccgggcg gagacccggg ctttcctgtt
     2941 cggcgagggc tgagcgcgcg ggggtcgcta gtaagcggac gcccctggca gataacgctc
     3001 ccggtcgcgg acgtcgaatt tcaggttgag gtgctcgagg ccccgcagga agctgctggg
     3061 gacgtgcagg aatcggtcgc ccggatccgc caggcggatg ttgtccagcc ggtgcagcag
     3121 gatggtgaat gccgagaaca tctcctgcct ggccagggcc gcgccggggc agtggtgcgg
     3181 accggagccg aacgccagat gggcgcccgc tttctgacgc tcgatgtcga agacctcgga
     3241 atcctggaaa acgtcctcgt cacgattcgc tgaagcgtaa ccgatggcca ccatggatcc
     3301 ttggggaata aacgccccgc caagatcggt atcctgcgtg gtgatccgcc acagatggag
     3361 cacgggtgtc tcaaagcgca gcgactcttc gaggaagttg cggataagct tctggtcgtc
     3421 ccgcatcttc tccatcagcc ccg
//
LOCUS       DBNHLM010000235        21995 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_931672, whole genome shotgun sequence.
ACCESSION   DBNHLM010000235 DBNHLM010000000
VERSION     DBNHLM010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 21995)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21995)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21995
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_931672"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..554)
                     /locus_tag="AC16QF_10775"
     CDS             complement(120..554)
                     /locus_tag="AC16QF_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009686074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6449083.1"
                     /translation="MTRQEHKGRCHCGAVRFEVRLTDGLSTARRCNCSLCRMRGAIAV
                     TANVGDLNILQGADRLTTYQFNTRTAKHYFCSVCGIYTHHQRRSNSVEYGINAACLEG
                     VSPFDFPDVVVYDGVQHPSDLKHATGPKVAGHLRFFAADAAG"
     gene            655..1188
                     /locus_tag="AC16QF_10780"
     CDS             655..1188
                     /locus_tag="AC16QF_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449084.1"
                     /translation="MDHQAFAEMLGNYGEFVGAIAVVATLAYLAIQIRQNTRAMQSTA
                     ELEAARHWSEQNIRAALDPDMTRIADVGNRDAAALNDDERRRYIWFLAHHWYMVDGLF
                     KLHQKGQLSDDAWHPFERQIRGSLSLESIQIWLRSGISPLSDSFRQHFGISAPETWSH
                     SEDLARMFDPGGSSPSD"
     gene            complement(1730..1806)
                     /locus_tag="AC16QF_10785"
     tRNA            complement(1730..1806)
                     /locus_tag="AC16QF_10785"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1770..1772),aa:Val,seq:gac)
     gene            2220..2735
                     /gene="infC"
                     /locus_tag="AC16QF_10790"
     CDS             2220..2735
                     /gene="infC"
                     /locus_tag="AC16QF_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR6449085.1"
                     /translation="MERSLLNEEIMARSVRLIGADGGQVGVVSRDEALDEAKRSGLDL
                     VLIAPEADPPVVKVMDYGKHLFELKKTKAAQKKKQKQMQVKEMKFRPGTEEGDYQVKL
                     RNLIRFLEDGDKAKVSLRFRGREVVHPEIGMNLMSRIEKDLEEYGLVEAEPKFEGRQV
                     VMVLGPKKKKK"
     gene            2774..2974
                     /gene="rpmI"
                     /locus_tag="AC16QF_10795"
     CDS             2774..2974
                     /gene="rpmI"
                     /locus_tag="AC16QF_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR6449086.1"
                     /translation="MPKMKTKSGAAKRFRRTANGFKHRKANRNHILTKKAPKRKRQLR
                     GLHDVSAADAPAINRMIPKKAR"
     gene            2984..3349
                     /gene="rplT"
                     /locus_tag="AC16QF_10800"
     CDS             2984..3349
                     /gene="rplT"
                     /locus_tag="AC16QF_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR6449087.1"
                     /translation="MPRARNTVASRTRRKKVLKQAKGYYGARSRSFKVAKQAVIKAGQ
                     YAYRDRRQRKRQFRSLWVVRINAAAREHDLSYSRFIAGLKKAAIEVDRKVLADLAVHE
                     KETFAVLAEQAKQALAEAA"
     gene            3479..4492
                     /gene="pheS"
                     /locus_tag="AC16QF_10805"
     CDS             3479..4492
                     /gene="pheS"
                     /locus_tag="AC16QF_10805"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010209123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR6449088.1"
                     /translation="MNIEELLEQARTGIAEAGDEAALDAVRVRFLGKKGELTVLLKSL
                     GTLDAEERPKAGAAINLAKEQVQEWIAQRRASLGSEQLAQALASESVDVTLPGRGERP
                     GGLHPVTQAMERIEDIFTGAGYSVVPGQEIEDDYHNFEALNIPAHHPARAMHDTFYFG
                     NGLLLRTHTSPSQVHVMENQAPPIRVICPGRVYRRDSDLTHTPMFHQVEGLVIDRGIS
                     FADLKGTVVGFLQRFFEQDLAVRFRPSYFPFTEPSAEVDVQCVHCLGKGCRVCSQTGW
                     LEVMGCGLVHPNVLEMSGVDSEEFSGFAFGMGGDRLAMLLYEVDDLRMFFDNDLRFLR
                     QFT"
     gene            4511..6889
                     /gene="pheT"
                     /locus_tag="AC16QF_10810"
     CDS             4511..6889
                     /gene="pheT"
                     /locus_tag="AC16QF_10810"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020300162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR6449089.1"
                     /translation="MKFSEAWLREWADPGISREALLDQLTMAGLEVDGVEPVSKEFAG
                     VVIAEVVSVDAHPNADKLSVCTVDDGTQTFQVVCGAPNVRAGMIGAFARVGAVLPDDF
                     RIKAAKLRGVESQGMLCSAAELGMGDDHSGILDLDCPDRVGDDLRQALALDDVIVDLD
                     LTPNRGDCLSVRGLAREVGVLNNVPVAAGEVEAVAPVLEDTFSVEIQDPAGCPRYLGR
                     VIRGIDPSCASPLWLTEKLRRCGVRAIDPVVDVTNFVMLELGQPMHAFDLGQLRDGIV
                     VRRARDGEQLRLLDGQELALDADTLLITDGSGPVAIAGVMGGERSGIQAATTDVFLEC
                     AYFAPLAVAGTARRYALHTDASHRYERGVDFELQHAAMERATRLLLDIVGGEPGPVVE
                     AVEPKALPEVSDIGLRERRLNRLLGTEIAPEIVDEAMERLDFAVTSRETGEEGVRWTV
                     RVPSHRFDIVREADLVEEVCRIYGYNNVPVRQPVTELELTPVPLERSPAGRLKRLLAT
                     LGYQEAITYSFVDPRLQDLLAPAADALALSNPMSSEQSVMRLNLLPGLVEALRFNVAR
                     QQDRVRIFELGSCFAPGDPVLQKLMLGGVLWGRRHGENWNQSGAEVDFYDLKGDVEAI
                     VGWSGEPVAYERLEDPVLHPGQAALVVGSRGPVGRLGRLHPEIERALDLPGHVFIFEL
                     EADAVLHRARRRHLSLSKFPSVRRDLAVVVQADTASSSIERILRESLGEILVDFRIFD
                     VYQGKGIDSNEKSLGIGLTLQHPSATLNDQEIGKYMNEAVDALVRGVGARLR"
     gene            6953..7258
                     /gene="ihfA"
                     /locus_tag="AC16QF_10815"
     CDS             6953..7258
                     /gene="ihfA"
                     /locus_tag="AC16QF_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HIR6449090.1"
                     /translation="MGALTKAQMADRLFDELGLNKREAKEIVEMFFDEVRDSLENNQQ
                     VKLSGFGNFDLREKGERPGRNPKTGEEIPITARRVVTFRPGQKLKARVEAYAGPGSD"
     gene            7236..7592
                     /locus_tag="AC16QF_10820"
     CDS             7236..7592
                     /locus_tag="AC16QF_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6449091.1"
                     /translation="MLDPGQTDELPPIPGKRYFTIGEVSQLCEVKPHVLRYWEQEFPQ
                     LKPVKRRGNRRYYQRQDVMTIRQIRSLLYDQGFTIGGARQQLAGADAKRDHSQFKLLV
                     RQMIVELEDLLRVLKS"
     gene            complement(7849..9102)
                     /locus_tag="AC16QF_10825"
     CDS             complement(7849..9102)
                     /locus_tag="AC16QF_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449092.1"
                     /translation="MSEIDEKEVRQEVGTWLEANWDPQLGLLEWRNILVDSGWGMPHW
                     PTQWHGRGLPVGLTPAVDDELASVNAVSVAKSGIRLLAAATLLEHGSDMHREKFLRRI
                     LTGEDTWCQLFSEPGSGSDLAGATTRADFDGTSWIINGQKVWTTSAHHAQWGLLLART
                     DWDVPKHQGLSYFVLDMSQPGVEVQPLRQMNGHASFNQVFITDAKVAPEFQVSTTGNG
                     WAVAMTTLAHERRGADGLRHAARAKDLPGRIYEEERAELATVMEPYKWYPQRAGRIDL
                     VIDRASATGALAQPNVRQAMARALIMSRSAEWTARRARAAQQLGRPQGPEGSLGKLAA
                     SNVARACSHVHTLISGTDAMLTGPESPLGGVVSEILLSVPATSIAGGTDEVQRNIISE
                     RVLGMPKEPRFDTGPFRDVPKNVLT"
     gene            complement(9114..10244)
                     /locus_tag="AC16QF_10830"
     CDS             complement(9114..10244)
                     /locus_tag="AC16QF_10830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449093.1"
                     /translation="MSFSFTDEQEQFREATRRFLQDKSPTTEVRRLMETSEGFDEAVW
                     KQLSGELALPGIHIPEQYGGAGFGPVELGIIMEELGRSLLCAPYFGSSVLAATAILEA
                     ATEDWKCELLPALAAGEQLGALAVTETGGRWDSDGIATVARDSGAGFSLTGTKSFVVD
                     GLTAGFLIVAARTPDTGGEAGVGLYLVRPDDAGVTRTPLETMDATRKLARIDLLDAPA
                     RPLGEPGADWPALQRTLDLAAVALANESVGGAQVMLDSSVEYSKIRMQFGRAIGSFQA
                     IKHRCADMLVEVELAKSAAYYAAQAAASGDPELPALACLAKAAASDAFVSTAASCIQI
                     HGGIGFTWDHDTHLWFKRAKSSEVFLGTPDHHRELYLQRTGV"
     gene            complement(10263..11447)
                     /locus_tag="AC16QF_10835"
     CDS             complement(10263..11447)
                     /locus_tag="AC16QF_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449094.1"
                     /translation="MSELSEFRNQTRAWLETSCPAGARGPGQIPWGSSKIPLESDVRL
                     WLERMAERGWTVPTWPKEYGGAELSRDQYMVLIDELKRINARTPLTGRGVNYIGPTIL
                     EFGTHEQKERWLPGIARGEGGWAMGYSEPGAGSDLASLSTRAELQGDQYVLNGLKTWT
                     SDAMDCDYIFVLARTDPDRPKHQGISLLLVDMNQPGVQVRPIRLLSGASPFNETTFEN
                     AVARADDVIGEVNAGWTVGKRLLQFERSTHAGINISGSQGGRSAESNLAERLKQFVPS
                     RDGKLQDPELRERLVRYEQNGHAQRLTQKRVIEELKARAPGYASSAVKLTGALHTQEG
                     DELLLAAMGTQGIVWDGPGLERSDSEALRTWLRQKSLTIAGGTKEVQLNIIAKRVLGL
                     PD"
     gene            11532..12305
                     /locus_tag="AC16QF_10840"
     CDS             11532..12305
                     /locus_tag="AC16QF_10840"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR6449095.1"
                     /translation="MKIATWNVNGLNARQEFVGLWLAHRQPDLVGLQEIKLVDEAFPA
                     DFFAGLGYQVLTHGQKAWNGVAVLSRLPVEIVQKGLPGQESAGSRLITVRVDDRLEFT
                     TVYCPNGKTLDHEDYAKKLAWFTSLSEYWRDRPSAGAVLCGDFNVVPEAVDSWRGEAG
                     DGRIFHTLEERAAFRSLLDAGLEDLFRMAKPDEQAFSWWDYRGGSFHRGHGLRIDLIL
                     GSRDVAERVVDVRTDRDYRKKKDGLTASDHAPVIAEIDL"
     gene            12465..13016
                     /locus_tag="AC16QF_10845"
     CDS             12465..13016
                     /locus_tag="AC16QF_10845"
                     /inference="COORDINATES: protein motif:HMM:NF039725.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6316 family protein"
                     /protein_id="HIR6449096.1"
                     /translation="MLPEWFRSDEIFSDEGRWYFGARGGLHVGPYGDRELASARSLSV
                     SRRLRALRTDADRLRYVRKVLHEEWESLGVSASAAEAETTIDLSPPSEPVRSGERQRK
                     WCRSSRFFQIHGVWFVSTREGINIGPYGTQFEARKHERHLIALLVRARDPAEARRVIY
                     RFKHRPNLTGTPWSQTDEVARTG"
     gene            complement(13040..14218)
                     /locus_tag="AC16QF_10850"
     CDS             complement(13040..14218)
                     /locus_tag="AC16QF_10850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015270460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449097.1"
                     /translation="MNQPDTALSPKVSELVARTTEFVTDKVIPYEKDPRNGSHGPSDA
                     LRVELQTAAREAGLLVPQLAEHLGGHDLNHREMAAVFRAAGYSPLGPIALNIMAPDEG
                     NMHLLHKVATPEQQERFLQPLAAGTIRSAFLMTEPDGGAGSDPGMLATMAEREGDDWV
                     INGRKSFITGGEGAGFGIIMAKTGSSATMFLADMNTPGISIERVLDTIDSSMPGGHAV
                     VRLDGVRVPADQILGELDEGFRYAQVRLAPARLTHCMRWWGSAQRAHDIASDYACRRQ
                     AFGKPIIDHEGVGFMLADNLMDLKQAELMIDWCAGVLDSGAPGTAESSMAKVAVSEAL
                     FRVADRCVQVLGGTGVTQDTIVEQVFREIRAFRIYDGPSEVHRWSLAKRIKRERLAST
                     "
     gene            complement(14215..15099)
                     /locus_tag="AC16QF_10855"
     CDS             complement(14215..15099)
                     /locus_tag="AC16QF_10855"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6449098.1"
                     /translation="MGRPTDLGVIDLMLEMPTGTAGMGMKQARALTRDKGTGEFSHHP
                     AQYLFKDAPERMSLATDPDAVVGMMDDFGVAMAQIAVNPRKPEAALDLFEKHPGRFFG
                     EVGVDPNSGMEGVRALETCVGLHPNIKAASAAPCLLNPQVPIDDKKFYPIYAKCCELD
                     IPINMLVGVPGPRVPYRCQHPGLLDEVAWFFPELKVVMRHGGDPWTDLCVKLLLKWPN
                     LYYSTSAWAPKHYPRNVLEFANKRGRGKVLYAGYFPGLSYERIFTEMDDLPLTDDTWP
                     AFLRDNAINVYKLQDILE"
     gene            15207..15836
                     /locus_tag="AC16QF_10860"
     CDS             15207..15836
                     /locus_tag="AC16QF_10860"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6449099.1"
                     /translation="MIRIYHVAGTRSVRVIWLCEELGLPYEKVAVDFSPAYRATPEWR
                     RLNPVGKVPVLVDDDVTMFESGAMVEHLLDRHGAGRLRPPPGTAERAAYLQWSWFAEA
                     TFARPLGEIVNHRREFPGESEIPAVLEEMSARAALCVQALDEALAGRQYLLGDEFTAA
                     DVMMGYSVLLARALGIEGARAGRVGAYFDVLARRSGYPAATADLPDAWR"
     gene            15882..16745
                     /locus_tag="AC16QF_10865"
     CDS             15882..16745
                     /locus_tag="AC16QF_10865"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6449100.1"
                     /translation="MSRSTFDVRPLDASFGAVVTGLRLADLGDDAFFDLYDAWLQHAL
                     LIFPDQHLSNAEQVRFAQRFGALEFDLTPLSNVRGDGSLRPDDGSDDVVKVLRGNMGW
                     HCDSTYMPVQAKGAVFTAHVVPDEGGETGWADMRAAYDALDEEMRERIADLCAYHSLY
                     HSQAKVGHVPSSESAYSNYGFHEGEPPLRPLVKIHPETGRPALMVGRHAYGIPGMDAD
                     ASERLLDELVEFACRPPRIYHHTWSPGDAVVWDNRCLMHRACPWDMSKPRVMYHARIA
                     GDPASESGLAA"
     gene            complement(16802..18091)
                     /locus_tag="AC16QF_10870"
     CDS             complement(16802..18091)
                     /locus_tag="AC16QF_10870"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR6449101.1"
                     /translation="MASADGSRGLIKTPVAKLSIGMYVAKLDRPWLESSFEVQGFYVN
                     NRALIEKLGAECEFVFVDPRRFKEIAEKPRLRVVAHNNVPRPASEGPAPTGRAKLRPV
                     KPRSPAVYEDTVETAAEIGVAQTSIDQAIEIMQPVVERLLATGRLESDQIEAAVTPLV
                     SSVLRNKDAVAALLRIRSLDDYTYSHSISNAVWAAVLGRHLGFSADQINTLSLGCALV
                     DIGKVTLPRILLSKPDPLTEAEEAAFRGHVEAGVEMIRQSTLNDRKVVMMVATHHERI
                     DGSGYPRGLAGDDIPTFGQIAGIIDTYDAMITERRYAPAMSSYEAVYQLQTYAGIKFQ
                     EELVEQLTQAIGVFPTGTLVELNTGEVGVVIAQNPERRLRPKINLILNEEKNLRRLPI
                     ITDLNAMDTDPATPPRVWIKRELPHGAYGIEATDYFI"
     gene            18301..18732
                     /locus_tag="AC16QF_10875"
     CDS             18301..18732
                     /locus_tag="AC16QF_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449102.1"
                     /translation="MCKPKSVHARNFLVAIALTFLFLGNAGCQSMDREEIAWQALHAV
                     DVAQTMSAARDPCYREAAWLTRNIIGEQPSEVEVLAWGVGTAIIHRWVGQVLERRDAP
                     RWVRVAWDLGTIGYTGHTVVDNHRNGVRIWGDNVEHDGCSA"
     gene            18734..20152
                     /locus_tag="AC16QF_10880"
     CDS             18734..20152
                     /locus_tag="AC16QF_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR6449103.1"
                     /translation="MSDRAGSGTDVAELFSRFGVPGGAVVLVSPRGEESLTYGSVSVA
                     RPGPVGANTLFNVGSVAKTIVAAATGLLVAERRMQLDDRLVDWVPEFTFPEPHLRTRI
                     TLRDCLAHRLGLAREGFWEWGARESLELTTLLGRLRHASMARPYRSGYAYSNVGYAAV
                     ALAASRAAGMPFLDYLRLWLDLPGDACLTGGRAARESERLSACHVLTPGPRAVRPHFA
                     DNVLGGSGIWASADFARVWLRLQLGATPTSGRCRQLPGRIRRELRLPHQRVPLADRPF
                     GVGGEACSAVHYALGWFVTRYRGHRVLRHSGAEVGSSAHLAIAPDAGIGVFVMANAES
                     PAADVIGLTLIDHLLGLRPLPWATRAPMGTRAPNGSQPRLNVRSTRAHPDGLRGLTGT
                     FRSPANGPVRITVQHGRLQMAFRDAPRFDGELVPLAGGWFRPRPADPGVRSLMEEPVR
                     FEDRSDGSCALRLGGLGTWQRR"
     gene            20228..20662
                     /locus_tag="AC16QF_10885"
     CDS             20228..20662
                     /locus_tag="AC16QF_10885"
                     /inference="COORDINATES: protein motif:HMM:NF037928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HIR6449104.1"
                     /translation="MAQTWLIVADGARARFFERRSASDLSEFDVMISPEGRSEERSLH
                     SDRPGRSFDSRGDGRHAMEPAHSARDHEAEAFAKRVCKRLDEARLAGSVDKLVLIAPP
                     RFLGHLRASLSKGTQGIVAHSIDKELTQASPDEIAEHIPGVL"
     gene            20695..20946
                     /locus_tag="AC16QF_10890"
     CDS             20695..20946
                     /locus_tag="AC16QF_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449105.1"
                     /translation="MDIDEVVSALRTERDELRVRLHLLKAEARDEFEELEKKWQHLES
                     RAAQVKEGAKESAEEIGAAAKTLAEEIGAAYTRIKKTLS"
     gene            21020..>21995
                     /locus_tag="AC16QF_10895"
     CDS             21020..>21995
                     /locus_tag="AC16QF_10895"
                     /inference="COORDINATES: protein motif:HMM:NF016032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HIR6449106.1"
                     /translation="MGDEIDRRYYDAEDFSDFRRRLDDETALLSDLFRRSEFSRRGDV
                     AGFELEVWLVDDEENPAPINDEYLKCLNNPLVVPELAEYNVELNGSPSALRGRVFSRL
                     HDELSATWRACRQAADRMGCALVTIGILPTVTEADLSSRYMSHMVRYQTLNDRVMALR
                     DGRPLELEIEGEGRLSTKHSDVMLEAAATSFQIHLQCRPDWAVRDFNASIVASAPIVA
                     ASANSPFLFGQSLWDETRIPLFEQAVDVGPNNPSRVSFGQGYIRESLLEIFEENRRDH
                     LILIPAVQDAPASKFSHVRFHNGTLWRWNRPLIGFDFDGQVHLRIEHRV"
BASE COUNT         3865 a         6845 c         7272 g         4013 t
ORIGIN      
        1 agatctggga agagtggcag ggctactaca cgagcagtta ccagaaatac gtcgacgacc
       61 tccgaaggca ggatgtcgcg gcaatttacg ctcagcgccg gcactattga gggcctgcat
      121 cagccagcag cgtcagcggc gaagaacctc aagtgtcccg ccactttggg ccccgttgca
      181 tgcttcaggt ccgatggatg ctgaacgccg tcgtagacca cgacatccgg aaagtcgaag
      241 gggctcaccc cctccagaca cgccgcgttg atgccgtact ctacggagtt tgatcgacgt
      301 tggtggtgcg tgtagatccc gcagaccgag cagaagtaat gcttcgctgt ccgggtgttg
      361 aattggtacg tcgtcaatcg atcggccccc tgcagaatgt tcagatcgcc aacgtttgcc
      421 gtcacggcga ttgcgccgcg catccggcat agagagcagt tgcatcggcg cgccgtgctc
      481 aaaccatcgg ttaacctgac ctcgaatctg acggcaccgc agtggcaacg acccttgtgc
      541 tcttgtcttg tcacgactct tctctctttg tgaacgggca gatggtagcg cagaaggctt
      601 gcggcgcaga ccggctgagg tagcctcgtt ccattcattg gagaggggac aatcatggat
      661 catcaggctt ttgcagagat gcttggtaac tacggggagt ttgtcggcgc catcgcggta
      721 gtagcgacct tggcctatct cgcaattcaa attcggcaga atactcgagc gatgcaaagc
      781 acggctgagc ttgaggccgc gcgccattgg tcggagcaga atatcagggc ggccctcgat
      841 ccagatatga cgcggatcgc tgatgtcggc aatcgagatg ctgctgcgtt aaacgacgat
      901 gaacgcaggc gatacatctg gttcctcgcg catcactggt acatggtcga cggtctcttc
      961 aagcttcacc agaagggaca gctctcagac gacgcatggc atcctttcga acggcaaatc
     1021 cgaggatcgc ttagcttgga gtcaattcag atatggttga gaagcgggat ttcgcctctt
     1081 tccgattcgt tccggcaaca tttcggtata tccgcaccgg aaacatggag ccattcggaa
     1141 gacctcgctc gaatgtttga tcctggaggc tcttcgccga gcgattgacc tgcgctaggc
     1201 ttcctcaatc accccgattc cattcgcgcc ggtgctggac gatctgtatc tgccgggtca
     1261 ggcagaaaac atggcggccg tgggcgagac cgggctgatc ggagagctac gcgctaccgc
     1321 cggcgggtct caagccactg tttgagggac tccagcgatt cgcgataacc cgcatcagcg
     1381 gcttgctgga ccgcatcgaa ttgcagcatg cctgttgatc ggcgtggaat attgaccccc
     1441 tttgcggggt aatcggcgtc caaaattgac cctccctgcg aagtgtagat cactccgagg
     1501 ctttggcttt ggagcggcga agggagatgt tgatcgtgga gacgattggg aagatccgcc
     1561 gggattactt tgttcatggc aagacgatca aggcggttgc gcgcgagcgt gggctgtcac
     1621 gcaataccgt gcgcaaggtg ctgagagcga ggtcacccga atctgcactc catacgcact
     1681 ccacgttatc gagcgattcg ggcgaactat cctaaccaat tgatttaatt ggtaggcgcg
     1741 attggaatcg aaccaacgac ctctaccatg tcaaggtagc gctctaacca actgagctac
     1801 gcgcctagcg tttgcgggac cggaatgtgc ccgttgcggg tcaacgcctc ggctccggac
     1861 gggcaatcat acaaaaatcg gccgggttgc aaaattgaat cggcggcatg gattaactca
     1921 ccttacccgt cttcggcgca ccgtggccgc ggccgcatca gaattttgct cgcggggaca
     1981 atcagttagc gcacttcggg cttgtgcggc cggtcgcaca ggcattagaa tccgccccct
     2041 gccgctcagc ggtgggccct gtttccgtgg agatggggga aataaatggg aatgggtcga
     2101 gcgcaaaccc cggcgagggc gcgtgtcggc ccgttttcgt aggaaaaagc gcattgaggt
     2161 attaacctcg cgctggggat tgatggagaa cgcctattaa gagaggtgga gcgaagcgcg
     2221 tggagcgatc tctcctcaac gaggagatca tggctcgcag cgtgcgtctt atcggcgctg
     2281 acggcggtca ggtcggtgta gtttcccggg acgaagccct ggacgaagcc aaacggtcgg
     2341 gtcttgatct ggtgctcatc gcgccggagg ccgacccgcc agtcgtcaag gtcatggact
     2401 acggcaagca tctgttcgag ctgaaaaaga ccaaagcagc tcagaagaaa aagcagaagc
     2461 agatgcaggt caaggaaatg aagtttcggc ccggaacgga ggaaggcgat tatcaggtca
     2521 aactacgcaa cctgattcga ttcctcgagg acggagacaa agccaaggtt tcgctgaggt
     2581 tccggggcag agaagtcgtg cacccggaaa tcggtatgaa cctgatgtcg cggattgaga
     2641 aggacctcga agaatacggg ttggtggaag ccgagccgaa gttcgagggg cggcaggtgg
     2701 tgatggtctt gggtcccaaa aagaagaaga aatagaacag catcatacaa gtctggaaat
     2761 tgcgagtaag ttcatgccga aaatgaagac gaaaagcggc gcggcgaaac ggtttcgccg
     2821 cactgcaaat ggtttcaagc accgcaaggc gaatcggaac cacatattga ccaagaaggc
     2881 gccgaagcgg aaacgccagc ttcgcggcct tcacgacgta tctgcggctg atgccccggc
     2941 gatcaatcgc atgattccca aaaaggcgcg ataggaggac gggatgccca gagcaagaaa
     3001 taccgtcgcc tcgcggacgc gccggaaaaa ggtgctcaag caggccaagg gctattacgg
     3061 agcgcgcagc cggtccttca aggtcgccaa gcaggcggtc atcaaggccg gacagtacgc
     3121 ctaccgcgat cgccgtcaac gcaagcgcca gttccgttcg ttgtgggttg tcagaatcaa
     3181 cgccgcggca cgcgagcacg acctttccta cagccgattt atcgccggcc tcaagaaggc
     3241 ggcaatcgaa gtggatcgaa aggtgctcgc cgatctggcc gtacacgaga aggaaacctt
     3301 cgccgtgctg gccgagcagg ccaagcaggc tctcgcggaa gcagcctgaa gcactgatcg
     3361 gtaaacatca gccgtcttct gggggccgtc gaagggctct tcccggaagc ggacgcgtac
     3421 gagcgaatct agggaaggag ccggcagcgg tacttccctt ttttcgtttg agaccgagat
     3481 gaacatagaa gagctactcg agcaggccag gacgggaatc gccgaagcag gggatgaagc
     3541 ggctctcgac gccgtgcgcg ttcgctttct cggcaagaag ggggaactca ccgttctcct
     3601 gaagtccctg ggtactctcg atgccgagga acgacccaag gctggcgcgg ccatcaatct
     3661 tgcgaaggaa caggttcagg agtggatcgc gcagcgccgc gcgtcgctcg gctccgagca
     3721 gcttgcccag gcgctcgcca gcgaaagcgt tgacgtgacc ctgcccggta ggggagagcg
     3781 gcctggaggc ctgcatccgg ttacccaggc gatggagcgg atcgaggaca tcttcaccgg
     3841 agccggctac agcgtcgtcc cgggccagga gatcgaggac gactatcaca atttcgaggc
     3901 tctcaacatt cccgcgcacc atccggcccg ggccatgcac gacaccttct acttcggcaa
     3961 cggcctgctg ctgaggaccc acacctcgcc gtctcaggtt cacgtcatgg agaaccaggc
     4021 gccgcccatc cgggtcatct gcccggggcg cgtctatcga cgggattccg atctcaccca
     4081 tacccccatg tttcaccagg tcgaagggct ggtcatcgat cgcggcatca gctttgccga
     4141 tctaaagggc acggtggtgg gctttctgca gcggtttttc gagcaggatc tcgccgtccg
     4201 gttccggcct tcgtacttcc cgttcaccga gccgtctgcc gaagtggacg tccaatgcgt
     4261 tcactgcctg ggcaagggct gccgggtctg cagccagacc ggctggctcg aggtgatggg
     4321 ctgcgggctc gtccacccga acgtgctcga aatgtcgggc gtggacagcg aggagttctc
     4381 ggggttcgcc ttcggcatgg gcggcgaccg gctcgccatg ctgctctacg aggtcgacga
     4441 cctgcgcatg ttcttcgaca acgatctccg cttcctgcgg cagttcacgt gaccgggtcg
     4501 gggggcgatc atgaagttca gtgaagcctg gctgcgcgag tgggcggacc caggcatctc
     4561 cagggaggcg ctgctggacc agctgaccat ggccgggttg gaggtggacg gcgttgagcc
     4621 cgtatccaaa gagtttgccg gcgtcgtgat agctgaggtg gtctcggtgg acgcccaccc
     4681 caatgcggac aagctttcgg tttgcaccgt cgacgatggc acacagacct tccaggtggt
     4741 gtgcggcgcg ccaaacgtcc gggcgggcat gatcggcgcc ttcgcccggg tgggggccgt
     4801 gcttccggat gatttccgca tcaaggcggc caagcttcgc ggagtcgagt cccagggcat
     4861 gctctgcagc gcggccgagc tcggcatggg cgatgaccac agcggcatcc tcgacctcga
     4921 ctgccccgac cgcgtgggcg atgacctgcg acaggcgctg gccctcgacg acgtcatcgt
     4981 cgacctggac ctcacgccga accgcggtga ctgcctgagc gtgcgaggtc tcgcccggga
     5041 agtgggggtg ctcaacaacg ttccggtcgc cgccggcgag gtggaggccg tcgcgcccgt
     5101 gttggaggat acgttcagcg tcgagatcca ggatcccgca ggctgcccgc gatatctggg
     5161 acgggtaatt cggggcattg atccctcctg cgcgagcccg ctgtggctca ccgagaagct
     5221 gcgccgctgc ggcgtgcgcg ccatcgaccc ggtggtggac gtcaccaatt tcgtcatgct
     5281 cgagctgggt cagcccatgc atgccttcga cctcgggcag cttcgcgacg gcatcgtggt
     5341 ccggcgggcg cgggatggcg agcagctgag gttgctggat ggccaggaac tggccctgga
     5401 cgcggacacc ctgctgatca cggatggcag cggaccggtc gccatcgccg gggtgatggg
     5461 cggggagcgc agcggcattc aggcggcaac caccgacgtg ttcctggagt gcgcctactt
     5521 cgccccgctg gcggtagccg gcacggcgcg tcggtacgcg ctccacacgg acgcgtcgca
     5581 ccgctatgag cggggcgtgg atttcgagct gcagcatgcg gccatggaga gagcgacccg
     5641 gctgctcctg gacatcgttg gcggggagcc gggaccggtg gtggaggcgg tggaaccgaa
     5701 agcccttccg gaggtgtccg acatcgggtt gcgcgagcgt cggctcaacc gtcttctcgg
     5761 gacggagatc gcgccggaga tcgtcgacga ggccatggag aggctcgact tcgcggtgac
     5821 gtctagggag acgggagagg agggcgttcg ctggactgtg cgcgtgccca gccaccggtt
     5881 cgacatcgtg cgcgaagcgg atctcgtcga agaggtctgc cgcatctacg gttacaacaa
     5941 cgtgccggtg cgccagccgg tgaccgagct ggagctcacg ccggtgcccc tcgaaaggag
     6001 cccggcagga cggctgaagc ggctgctggc aaccctcggc tatcaggaag ccatcaccta
     6061 cagcttcgtg gacccgcgtc tgcaggatct gctcgcgccc gccgccgatg ccctggcgct
     6121 gagcaatccc atgtcgtcgg agcagtcggt catgcggctg aacctgctgc ccggcctcgt
     6181 cgaggcgctg cgtttcaacg tggcccggca gcaggaccgg gtgcggatat tcgagctggg
     6241 gtcgtgcttt gcccccggcg accccgtgct gcagaagctg atgcttggcg gtgtgctctg
     6301 gggtcggcgg cacggggaga actggaacca gtcaggcgcg gaagtcgact tctacgatct
     6361 caagggcgac gtcgaggcca tcgtggggtg gagcggcgag ccggtggcct acgagcggct
     6421 cgaagacccg gtgcttcacc ctggccaggc ggcgctcgtg gtgggttcgc gagggccggt
     6481 ggggcggctg gggcggctgc atccggagat cgaacgcgcg ctggatctgc cggggcacgt
     6541 cttcatcttc gagctggagg cagacgcggt tctgcatcgc gcccgccgca ggcatctgag
     6601 cctgtcgaag ttccccagcg tcaggcggga cctggccgta gtggttcagg ccgatacggc
     6661 ctcttcgagc atcgagagga tcctccgcga gtccctgggc gaaattctcg tcgacttcag
     6721 gatatttgac gtctatcagg gcaaaggtat tgattctaat gagaaaagcc ttggtatagg
     6781 gttgacgttg cagcatccgt cggccaccct taacgaccag gaaattggca aatacatgaa
     6841 cgaagccgtg gacgccctgg ttcgaggtgt gggcgcaagg ctgcgttgaa gaatcggacg
     6901 cccgtccccg tgatctcagg ggtcgggtgc cgaaaacaca ggagactccc gggtgggtgc
     6961 ccttaccaaa gcgcagatgg ccgaccgcct cttcgatgag ctcgggctga acaagcgcga
     7021 agccaaggag atcgtcgaga tgtttttcga cgaggtccgg gactcgctgg agaacaacca
     7081 gcaggtcaag ctctccggat tcgggaactt cgatctgcgt gagaagggag agcggccggg
     7141 ccgcaatccg aaaactgggg aagagatccc catcacggcc cggcgcgtcg tgacgttccg
     7201 acccgggcag aaactgaagg cgcgagtgga agcttatgct ggacccgggt cagactgacg
     7261 agctgccgcc cattcccgga aagcggtact tcaccatcgg tgaggtgagc cagctgtgcg
     7321 aggtcaagcc ccacgtgctg cgctactggg agcaggaatt cccccagctc aagcccgtca
     7381 agcgtcgcgg caaccgccgt tactaccagc gtcaggacgt catgaccatc cgccagatcc
     7441 ggtcgctgct ctatgaccag ggttttacga tcgggggcgc ccgacaacag ctggcgggcg
     7501 cagacgccaa acgagaccac tcccagttca agctcctggt ccggcagatg atcgtggagc
     7561 tcgaggacct gctgagggtc ctcaagtcct gactcccggg gcgcggcagc gatcttccat
     7621 gcatgacgat gatggcacgg caggcgcagg cagggaggag gggtccggct ccatacgggt
     7681 cgccatcgtg atcttcctgg ttacctttgt gatgctcatg gtcatgggga tcatctggat
     7741 gttcattcgc gtcgaaggct cgtcctctgg cggccaagct acgggcaccg gcgaaaacgc
     7801 gaccgtctgc cgttgcctgg ccgccgcctc cccagcgcgc ggcgagcctc aggtcagcac
     7861 gtttttcggc acgtcgcgaa acgggcccgt gtcaaagcgc ggctctttcg gcatgccgag
     7921 gacacgctcg gagatgatgt tccgctgcac ctcgtccgtg ccgccggcga tggacgtggc
     7981 cggcaccgac aacaggattt ccgacaccac gccgccgaga gggctctccg gaccggtgag
     8041 catggcgtcg gtgccgctga tcagcgtgtg aacgtgggaa cacgcgcgcg ccacgttgct
     8101 ggcggcgagc ttgcccagcg aaccttccgg gccctggggc cggccgagct gctgggcagc
     8161 acgcgccctg cgtgccgtcc actcggcgct tcgcgacatg atcagcgcac gtgccatggc
     8221 ctgccgcacg ttaggctgcg ccaaggcgcc cgtggctgat gcccgatcga tgactagatc
     8281 gatgcgaccg gcgcgctggg ggtaccactt gtacggctcc atcaccgtgg cgagctctgc
     8341 ccgctcctcc tcgtagatgc ggcctggcag gtctttcgcg cgcgcggcgt gacgcagccc
     8401 gtcagcgccg cgacgttcgt gggcgagcgt ggtcatggct acagcccagc cgttgccggt
     8461 cgtggatacc tggaactccg gtgcgacctt ggcgtcggtg atgaagacct ggttgaagga
     8521 cgcgtgaccg ttcatctgcc tgagaggctg cacctccacc ccgggctggg acatgtcgag
     8581 gacgaagtag gacagcccct gatgcttcgg cacgtcccag tctgtgcgtg ccagcagcaa
     8641 tccccactgg gcgtgatgag cgctggtggt ccacaccttc tggccgttga tgatccagct
     8701 ggtcccgtcg aagtcggcgc gggtggtggc accggcaagg tccgacccgc tgccgggctc
     8761 gctgaagagc tggcaccagg tgtcctcgcc ggtgaggatc cggcgcagga acttctccct
     8821 gtgcatgtcg ctgccgtgct cgagcagcgt ggccgccgcc agaagcctga tgcccgactt
     8881 tgccacgctc accgcgttga cggacgccag ctcgtcgtcc acggccggcg tcagccccac
     8941 cggcagtccc cggccgtgcc actgggtggg ccaatggggc atgccccacc ccgagtccac
     9001 gaggatgttg cgccactcga gcaggcctag ctggggatcc caattcgctt ccagccaggt
     9061 cccgacctcc tgacgtactt ccttttcgtc gatttcgctc atggtcaacg tcctcagact
     9121 ccggtccgct gcagatacag ttcccggtgg tggtcggggg tgcccagaaa gacctctgag
     9181 ctcttggccc gtttgaacca caggtgcgtg tcgtgatccc acgtgaagcc gatgccgccg
     9241 tggatctgga tgcagcttgc cgccgtgctc acgaacgcat cgcttgccgc ggctttggcg
     9301 aggcaggcga gggccggcaa ttcggggtcg cccgaagcgg cggcctgggc tgcgtagtag
     9361 gcggcggact ttgccagctc cacttccacg agcatgtcgg cgcaccggtg cttgatggcc
     9421 tggaaggatc cgatggcgcg gccgaactgc atgcggattt tggaatactc cacggagctg
     9481 tccagcatga cctgggctcc gcccacggat tcgttggcca gggccaccgc cgcgaggtcc
     9541 agcgttcgct gcagggcggg ccagtccgcg cctggctctc ccagcggccg tgcgggcgcg
     9601 tcgagcagat cgatgcgtgc gagcttgcgt gtggcatcca tggtttccaa cggggtccgg
     9661 gtgaccccgg catcgtctgg cctgacgaga tacaacccta cgccggcctc accgccggtg
     9721 tccggtgtcc gggccgccac aatcaggaag ccggccgtca ggccatcgac cacgaagctc
     9781 ttggtcccgg tcagcgagaa gccggcgccg ctgtcccggg ccacggtcgc gatgccgtcg
     9841 ctgtcccatc gcccgccggt ttcggtgacc gccagcgcgc cgagttgctc gcccgctgcc
     9901 agcgcgggca ggagctcgca cttccaatct tccgtagcgg cctcgagtat ggcggttgcg
     9961 gccagcacgc tggagccgaa gtacggcgcg cacaggaggg accgaccgag ctcctccatg
    10021 atgatgccga gctccacggg accgaatccg gcgccgccgt actgctccgg aatgtgaata
    10081 cccggcagcg ccagctcccc ggacagctgc ttccataccg cctcgtcgaa accctcagag
    10141 gtctccatca ggcgcctgac ctcggtggtc ggggacttgt cctgcagaaa tcgccgggtg
    10201 gcttcccgga actgctcctg ctcgtcggta aagctgaagg acatctcgca aactcctcga
    10261 ggtcaatccg gcaggccgag aacgcgtttc gcgatgatgt tcagctgcac ttctttggtc
    10321 ccgccggcaa tggtcaggga tttctggcgc agccaggtgc gcagtgcctc gctatcgctt
    10381 cgttcgagtc ctggcccatc ccagacgatc ccctgggtcc ccatggcggc gagcaggagc
    10441 tcgtcccctt cctgggtgtg cagtgcgccc gtcagcttca cagcggaaga ggcatacccc
    10501 ggcgcgcgcg cttttagctc ctcgatgacg cgcttctggg tcaggcgctg ggcgtggccg
    10561 ttctgctcgt agcgaaccag ccgctcgcgc agctcgggat cctgcagctt gccgtcccga
    10621 ctgggaacga actgcttgag gcgttctgcc agattgcttt cggcgctgcg gccgccctgg
    10681 gagccggaga tgttgatacc tgcgtgggtg gaacgttcga actgcagcag acgtttcccc
    10741 actgtccagc cggcgttcac ctcgccaatg acgtcgtcgg ccctggccac ggcgttctcg
    10801 aacgtggtct cgttgaaggg tgaggcgccc gaaagcaggc gaatggggcg gacctgcacg
    10861 ccgggctggt tcatgtccac gagaaggaga ctgattccct ggtgtttcgg gcggtcggga
    10921 tccgtgcggg cgagaacgaa gatgtagtcg cagtccatgg cgtcggatgt ccacgtcttg
    10981 aggccgttga gaacatactg atcgccctga agctccgccc gggtgctcag gctggccagg
    11041 tcggatccgg ctccgggctc ggagtacccc atggcccatc cgccttctcc cctggcgatt
    11101 cccggcagcc agcgttcttt ctgctcgtgc gtaccgaatt cgagtatggt cggaccgatg
    11161 tagttcacgc cccgtccggt cagcggcgtg cgggcgttga tgcgtttgag ctcatcgatc
    11221 agcaccatgt actgatccct gctcagctcg gcgccaccgt actccttcgg ccaggtcgga
    11281 accgtccagc cgcgttccgc catgcgctca agccacaggc gcacgtcgct ttccagcggg
    11341 atcttgctgc tgccccacgg tatctggccc ggcccgcggg cgccggcggg gcagctggtt
    11401 tccagccatg cccgcgtctg gttgcggaac tcgctcagct cgctcatctg gtttctctca
    11461 agcgctcgat ccagcgatgc acgccggttg aaacggcgat cacggcgacc gattaacgct
    11521 acaattcatc catgaaaatt gccacctgga atgtcaacgg gctgaacgcc cgacaggagt
    11581 tcgtcggttt gtggctggcc caccggcagc ccgacctggt cgggctgcag gagatcaagc
    11641 tggtcgacga agccttcccc gccgatttct tcgccgggct gggttatcag gtcctcaccc
    11701 atggccagaa ggcatggaac ggcgtcgcgg tgctcagccg cctgcccgtg gaaatcgtcc
    11761 agaagggact gccaggccag gagtcggcag gatcccgcct gatcaccgtg cgggtcgacg
    11821 atcgcctgga gttcacgacc gtctactgcc ccaacggcaa gacgctcgac cacgaggact
    11881 acgcgaagaa gcttgcctgg ttcacctcgc tcagcgagta ctggcgtgat cgcccctcgg
    11941 caggggccgt cctctgcgga gacttcaacg tggtgccgga agccgtcgac agctggcgag
    12001 gcgaagccgg cgacggcagg atttttcaca cgctcgaaga gcgtgcggcc ttccggtcgc
    12061 tgctcgatgc ggggcttgag gacctgtttc gtatggccaa gccggacgag caggcgtttt
    12121 cgtggtggga ctaccgcggc gggtcgtttc accgcggcca cgggctgaga atcgacctga
    12181 tactgggctc gcgcgacgtg gccgagcgcg tggtcgacgt ccgtaccgat cgggactatc
    12241 gcaagaagaa ggacggactg accgcctcgg atcacgcccc cgtcatcgcc gagatcgacc
    12301 tttaaaagat tggtcgcccc gctttcggga ctggctcaga ggccgcaggc tggacaccgc
    12361 ttggttaaat ggttcacggt ttgagggccc gaaaggggat atcgtctccc agcaggcaga
    12421 cctgggatca ggcttaccgg cagatttatt ggcggatgca cggaatgctt ccagagtggt
    12481 tcagatcaga cgagatcttc tccgacgagg ggcggtggta cttcggtgcc cggggcgggt
    12541 tgcacgtcgg tccttatggg gaccgcgagc tcgccagcgc ccgcagcctc tccgtctcgc
    12601 gccgcttacg ggcgctcaga accgatgccg atcggctcag gtacgtccgc aaggtgctcc
    12661 acgaggagtg ggagagcctg ggggtatccg ccagcgctgc ggaggcggaa acgaccatcg
    12721 atctctctcc tcccagcgag cccgtccgta gcggtgagcg ccagcggaag tggtgccgtt
    12781 cgagccgttt cttccagatc cacggcgtgt ggttcgtgtc cacccgggag ggcatcaaca
    12841 tcgggcccta cggaacgcag ttcgaggcca ggaagcacga gcgccacctc atcgcgcttc
    12901 tcgtcagggc gagagatccc gcggaggcca gacgggtgat ctatcgcttc aagcaccgac
    12961 caaacctgac gggtactccc tggtctcaaa ccgacgaggt agcccgcacc ggctgaacgc
    13021 ggcttcacgg cggcagctct caagtcgatg cgagccgctc ccgcttgatc cgcttggcca
    13081 gagaccagcg gtgaacctcg gaggggccat cgtagatccg aaaggctctg atctccctga
    13141 aaacctgctc cacgatggtg tcctgggtca ccccggtgcc gcccagcacc tgcacgcagc
    13201 ggtcggcgac acggaacagc gcctcggaga cggcgacctt cgccatgctc gactcggcgg
    13261 tgcccggcgc gcccgagtcg agcaccccgg cgcaccagtc gatcatcagc tcggcctgtt
    13321 tcaggtccat caggttgtcg gccagcatga agccgacacc ctcgtggtcg atgattggct
    13381 tgccgaaggc ctggcgccgg caggcgtagt ccgaggcgat gtcgtgggcc cgctgggcgc
    13441 tcccccacca gcgcatgcag tgggtcagac gcgcgggcgc caggcgcacc tgggcatagc
    13501 ggaagccctc gtccagctcg cccaggatct ggtccgccgg aacccgcacc ccgtcgaggc
    13561 gcacgacggc gtggcccccg ggcatggagc tgtcgatggt gtccaggacg cgctcgatgg
    13621 agatgcccgg cgtgttcatg tcggcgagaa acatggttgc cgacgacccg gtcttggcca
    13681 tgatgatgcc gaacccggcg ccttcgccgc cggtgatgaa cgacttgcgg ccgttgatca
    13741 cccagtcgtc gccctccctc tccgccatgg tggccagcat gcccgggtct gaccccgcgc
    13801 cgccgtcggg ctcggtcatc aggaacgcgg accggatggt gcccgccgcc agaggttgca
    13861 ggaaccgctc ctgctgctcg ggggttgcca ccttgtgcag caggtgcatg ttgccctcgt
    13921 cgggggccat gatgttcagc gcgatgggcc ccagcggcga gtacccggcg gcccggaaca
    13981 cggcagccat ctcccggtgg ttgaggtcat ggccgccgag gtgctcggcc aactggggca
    14041 cgaggaggcc ggcctcacga gccgccgtct gcagttcgac tcgcagagca tcgctgggcc
    14101 cgtgtgatcc gttgcgagga tccttctcgt agggaatgac tttgtcggtg acgaactcgg
    14161 tggttcgcgc gaccagttca gaaactttgg ggctcaacgc ggtgtccggc tggttcattc
    14221 gaggatgtcc tgaagtttgt agacgttgat ggcgttgtcg cggagaaatg ccggccaggt
    14281 atcgtctgtc agcggcaggt cgtccatctc cgtgaagatg cgctcgtagc tcaggccggg
    14341 gaaatagccg gcgtagagca ccttgccgcg gccgcgcttg ttggcgaact cgagaacgtt
    14401 cctggggtaa tgcttaggcg cccaggcaga cgtggaatag tagagattcg gccacttcaa
    14461 cagcagcttc acgcaaaggt ccgtccatgg gtccccgccg tgccgcatga ccaccttcag
    14521 ctccgggaaa aaccaggcca cctcgtcgag caggcccgga tgctggcacc tgtagggaac
    14581 gcgtggtccc ggcacgccga ccagcatgtt gatgggaatg tcgagctcgc agcacttcgc
    14641 gtagatggga tagaacttct tgtcgtcgat gggcacctga ggattcagca ggcagggcgc
    14701 ggcggaggcg gccttgatgt tggggtgcag cccgacgcag gtctcgagcg cgcgaacacc
    14761 ctccataccc gagttgggat cgacgcctac ctcgccgaag aatcggccgg gatgcttctc
    14821 gaaaagatcc agcgcggcct cgggtttgcg agggttgacg gcgatctgcg ccatggccac
    14881 gccgaaatcg tccatcatgc ccaccaccgc gtcgggatcg gtggccaaag acatgcgttc
    14941 cggggcgtcc ttgaagaggt actgggccgg atggtgggag aactcgcccg ttcccttgtc
    15001 ccttgtcaat gcacgggcct gcttcatgcc catgccggca gtgccggtgg gcatctcgag
    15061 catcaggtcg atcaccccaa gatctgtcgg tcgccccatc cgtgctcccc gtcggctgtc
    15121 tccaaagcgc gctcagttta accggttgcc gcgcggcgcg ctgttgcgga tgattgaagg
    15181 ccgcaagcca tggctgggaa gcccgcatga tcaggattta tcacgttgcc ggcaccagat
    15241 cggtccgggt catctggttg tgcgaggagc tcgggctgcc ttacgagaag gtggccgtcg
    15301 acttctctcc ggcctaccgg gcaacccccg aatggcgaag gctcaatccg gtaggcaaag
    15361 tgcccgtgct cgtcgacgac gacgtcacca tgttcgaatc cggtgccatg gtcgagcatc
    15421 tgctcgaccg tcacggagcg ggtcggctgc gtcccccgcc gggtactgcc gagcgtgccg
    15481 cctatctgca atggtcctgg tttgcggaag ccacctttgc ccggccgctc ggcgagatcg
    15541 tcaaccatcg ccgggagttt cccggcgaat cagaaattcc ggctgtgttg gaggagatga
    15601 gcgcccgtgc tgccctctgt gttcaggcgc tggacgaagc cctcgccggc cgccagtatc
    15661 tgcttggcga cgagttcacg gcggcggacg tgatgatggg ctattccgtt ctgctggcgc
    15721 gtgctctggg aattgagggg gcccgggccg gtcgtgtggg cgcatacttc gacgtgctgg
    15781 cccgaaggtc cggatatcct gcggcaacgg cggaccttcc cgacgcctgg cgctgacgct
    15841 gccgcgcccc ggaatgagcg atgaagaacc ggaggaaaag catgagcaga tcgacatttg
    15901 atgttcggcc gctggacgcc tcgttcggcg ccgtggtgac cggcttgcgc cttgcagacc
    15961 ttggggatga tgcttttttc gacctttatg acgcatggct gcagcacgcg ctgctcatct
    16021 tccccgatca gcacctgagc aacgccgagc aggtccgatt tgcgcagcgg ttcggagcgc
    16081 tggagttcga tctcacgccg ctgagcaacg tgcgcgggga cggcagcctg cgccccgatg
    16141 acggcagcga tgacgtggtc aaggtacttc gcggcaacat gggctggcat tgcgacagca
    16201 cctacatgcc cgtccaggca aaaggcgcgg tgttcaccgc ccacgtcgtc ccggacgaag
    16261 gcggcgagac gggctgggcg gacatgcggg ccgcttacga tgcgctggac gaggagatga
    16321 gggagcgcat tgccgacctg tgtgcctacc actcgctcta ccatagccag gcgaaggtgg
    16381 gtcatgtgcc aagcagcgaa agcgcctaca gcaactacgg gtttcacgag ggcgagccgc
    16441 ctctgcgccc tctcgtgaag attcacccgg agacaggccg gccggctctc atggtgggcc
    16501 gccatgccta cggcattccg ggcatggatg ccgatgcttc agagcgtctg cttgacgagc
    16561 tcgtcgagtt tgcctgccgc ccgccgcgca tctatcacca cacgtggtcg ccgggtgatg
    16621 cggtggtgtg ggacaaccgc tgtctgatgc atcgggcgtg tccgtgggac atgagcaagc
    16681 cgcgggtgat gtatcacgcc cgtattgccg gcgaccctgc ctcggagtcc ggactggccg
    16741 cgtgagcggt cgctctgccc cccgctcgca agatcggcgt caagcgcatc gagacctgcc
    16801 ctcagatgaa gtagtcggtg gcttcgattc cgtacgcgcc gtgaggcaac tcccgcttga
    16861 tccagaccct ggggggcgtg gcgggatcgg tgtccatggc gttgaggtcg gtgatgatgg
    16921 gcaggcggcg caggttcttc tcctcgttca gaatgaggtt gatcttgggt cgcagacgcc
    16981 gctcggggtt ctgcgcgatg accacgccca cttcgcccgt gttgagctcc acgagcgtgc
    17041 ccgtgggaaa cacgccgatg gcctgggtca gctgctcgac gagctcctcc tggaatttga
    17101 tgccggcata cgtctgcagc tggtagacgg cctcgtaaga ggacatggcc ggagcgtaac
    17161 ggcgttcggt gatcatcgcg tcgtaggtgt cgatgatccc ggcgatctgg ccgaaggtcg
    17221 ggatgtcgtc accggcgagc ccccggggat agcctgaccc gtcgatcctc tcgtgatgag
    17281 tggccaccat catgacgacc tttcggtcgt tcaaggtgga ttgccggatc atctcgacgc
    17341 cggcctccac atgcccgcgg aaggctgcct cttccgcctc ggtcagcggg tccggtttgg
    17401 aaagcaggat cctcggcagg gtgaccttgc cgatgtcgac cagcgcgcag cccagggaca
    17461 gggtgttgat ctgatccgcc gagaacccga gatgccgacc cagcacggcg gcccagacgg
    17521 cgttggagat ggagtgagag taggtgtagt cgtcgaggct gcggatgcgc agcagggcgg
    17581 ccaccgcgtc cttgttgcgc aacacgctgg agaccagcgg tgtgaccgct gcctcgatct
    17641 ggtccgactc gagccggccg gtcgccagga gccgctcgac cacgggttgc atgatctcta
    17701 tggcctggtc gattgaagtc tgggcgacgc cgatctccgc ggcggtctcc acggtgtcct
    17761 cgtaaacggc cggcgatcgc ggtttgactg gtctcagctt ggcgcgcccc gtcggggccg
    17821 gcccttccga cgccgggcgc gggacgttgt tgtgggccac cacccgcaac cggggcttct
    17881 ccgcgatctc cttgaaccgt ctgggatcga cgaatacgaa ctcgcactcg gcgccgagct
    17941 tttcgatcag cgcgcggttg ttcacgtaga agccctgcac ctcgaacgag gactccagcc
    18001 acgggcgatc gagcttggct acgtacatgc caattgagag tttggcgaca ggcgtcttta
    18061 tcaggccccg actgccgtcc gcgctagcca tcctggcgtc cttcgaccca ataaccaaca
    18121 cactagggta gtcgctgtgt ggaaactctc tgtgatccag ttcacccgga cgtcaacgcg
    18181 cccgacttct gctcacatgt ttctacctac agcggacaaa gcgggtacga agccaggaca
    18241 gcaatccggc tatgccagag ccactattga aggcgagaac cccaaaagga gcggcagctt
    18301 atgtgcaagc cgaagtccgt tcatgcgaga aattttctcg tcgccatcgc cctgacgttt
    18361 ctctttctgg gaaacgcggg ctgccagtcc atggatcgtg aagagattgc ctggcaggca
    18421 ctccacgcgg tagacgtggc gcagaccatg agcgccgccc gcgacccctg ctatcgagaa
    18481 gccgcatggc tgacccggaa catcattggc gagcagccca gcgaggtcga ggtgctggcc
    18541 tggggcgtgg gcacggcgat cattcaccgc tgggtcggtc aagtgctcga acgccgcgac
    18601 gcgccgagat gggtgcgggt ggcctgggat ctgggaacga tcggctacac cggccacacg
    18661 gtggtggaca accaccgcaa cggtgtgcgc atctggggcg acaacgtcga gcacgatggg
    18721 tgcagcgcgt gacgtgtccg acagggcagg gagcgggaca gacgtagccg agctgttcag
    18781 ccggttcggc gttcccggcg gggccgtcgt gctggtgtcc ccgagggggg aggagtcgct
    18841 cacctacgga agcgtatccg tcgcccgacc cggcccggtc ggggcgaaca ccctgttcaa
    18901 cgtcggctcg gtggccaaga ccatcgtggc cgctgccacc ggcttgctgg tggccgagcg
    18961 ccgaatgcag ctcgatgatc ggctggtgga ctgggttccc gagttcacgt ttcccgaacc
    19021 gcaccttcgc acccggatca cgctgcgcga ctgcctggcc cacaggctgg gtctggccag
    19081 ggaagggttc tgggagtggg gtgctcggga gtccctcgag ctcacgaccc tgctcgggcg
    19141 actgcgacat gcctccatgg cccggcccta tcgttccgga tatgcctact cgaacgtcgg
    19201 ctatgcagcc gtggccctgg ccgcctcccg ggcggccggg atgccgtttc tggactacct
    19261 tcggctctgg ctggatcttc cgggagacgc ttgcctgacc gggggccgcg cggctcgcga
    19321 gagcgaaagg ctctccgcct gccacgttct gacgccgggt cctcgtgcgg tccgcccgca
    19381 cttcgccgac aacgttctgg ggggctccgg aatctgggca tccgcggact tcgcccgggt
    19441 gtggctgcgc ttgcagctgg gcgcgacgcc gacgagcggc aggtgtcggc agctgcccgg
    19501 ccgcatcaga cgggagctgc gcctgcctca ccagcgggtg ccgctggcag acagaccatt
    19561 cggggtcggt ggcgaagcgt gctcagcggt gcattacgcc ctggggtggt ttgtcacgcg
    19621 ctatcgcggc catcgcgtgc tccgccacag cggcgctgaa gtcgggtcga gcgcccatct
    19681 cgccatcgcg cccgatgcgg gcatcggcgt gttcgtgatg gccaatgccg aatctccggc
    19741 cgcggacgtg atcgggctga cgctcatcga ccatctgctg ggtctgcgcc ccctgccatg
    19801 ggcgacccgc gcgcccatgg gaactcgagc gccgaacggc tcgcagccgc gcctgaacgt
    19861 gcgctccacg cgggcccatc cggatggtct tcgcgggctg acgggaacgt tccggagccc
    19921 ggccaacggg cccgtgcgga tcaccgtgca gcacgggcgg ctgcagatgg ccttccgtga
    19981 cgcgccgcga ttcgatggcg agctcgtgcc gctggccggc gggtggtttc ggccccgccc
    20041 ggcggatccg ggcgtgcgct cgctcatgga ggaaccggtc cgcttcgaag atcgaagtga
    20101 tggatcctgt gcgttgcgcc ttggcgggct cggtacctgg cagcggcgct gagtacgcgg
    20161 aaatccttaa agacactgcg gcacgacgca ggcaacctct cgtcaggttc aaagacggga
    20221 ggcttacgtg gcacagactt ggttgatcgt tgcggatggc gcccgggctc ggtttttcga
    20281 acgcaggagc gcttcggatc tcagcgagtt cgacgtcatg atttcgcccg aggggcgatc
    20341 cgaagaacgc tcgctgcact cggaccgtcc cggccgatcg tttgattccc gcggtgacgg
    20401 aaggcatgcc atggagcccg cccacagcgc ccgggatcac gaagcggagg cgttcgcaaa
    20461 gcgcgtctgc aaaaggctcg acgaagcgcg ccttgcggga tcggtggaca agctcgttct
    20521 catcgccccc ccccgttttc tggggcacct gcgagcgagc ctcagcaagg gtactcaggg
    20581 tatagtcgcg cacagcatcg acaaggaatt gacgcaggcc tcgcccgacg agattgccga
    20641 gcacataccg ggcgtcctct agggcgcctg acaacaactc tattaggagt gatcatggat
    20701 attgatgaag tggtttcagc cctgcgcacg gaacgggacg agttgagggt gcggttgcac
    20761 ctgctcaagg ccgaagctcg cgacgagttc gaggagctgg agaagaagtg gcagcacctc
    20821 gagagccgtg cggctcaggt aaaggaaggc gcgaaggagt ccgccgagga aatcggcgcc
    20881 gcagctaaga cgcttgccga ggagatcggt gccgcctaca cgcgaatcaa aaagacgctt
    20941 tcctaggaca tagacggacc tgccgccccg gcccggcgga gcggttgccg tcaggctgag
    21001 ctcgagacgc gggatttaca tgggcgacga gatcgataga cgctactacg atgctgagga
    21061 cttcagcgat ttccgccgcc gactcgatga tgaaaccgcg ctgttgagcg atctgtttcg
    21121 tcgcagcgag ttcagccgcc ggggcgacgt ggccgggttc gagctcgaag tctggctggt
    21181 ggacgacgag gaaaatcccg cgcccatcaa cgacgagtat ctcaagtgcc tgaacaaccc
    21241 gctcgtcgtt cctgagcttg ccgagtacaa cgtcgagctc aacggcagcc cgtccgcgct
    21301 ccggggccgg gtcttctccc ggctgcacga cgagctgagc gctacctggc gcgcctgcag
    21361 gcaggccgcg gatcggatgg gctgcgctct ggtgaccatc ggcatcctgc ctacggtgac
    21421 cgaggccgac ctcagctccc ggtacatgtc gcacatggtc cgctatcaga cactcaacga
    21481 tcgggtcatg gcgctgcgcg acgggcgtcc cctcgagctg gaaatcgagg gtgaagggcg
    21541 gctttccacg aaacactcgg acgtgatgct cgaagcggcc gccacgtctt tccagattca
    21601 cctgcagtgc cggccggact gggccgtcag ggacttcaac gccagcatcg tggcttctgc
    21661 gcccatcgtc gcggcaagcg ccaactcgcc gtttcttttc ggtcagtctc tttgggacga
    21721 gacccgcatc ccgctcttcg agcaggccgt cgacgtggga cccaacaacc cctcccgggt
    21781 gagcttcggt cagggttaca tccgcgagtc cctgctggag atcttcgagg agaaccgccg
    21841 cgaccacctc atcctgatcc ccgcggttca ggacgccccg gccagcaagt tcagtcacgt
    21901 gcgtttccac aacggcaccc tgtggcggtg gaatcgcccg ctcatcggct tcgacttcga
    21961 cggccaggtc cacctacgca tcgagcaccg ggtgg
//
LOCUS       DBNHLM010000236         4123 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_332747, whole genome shotgun sequence.
ACCESSION   DBNHLM010000236 DBNHLM010000000
VERSION     DBNHLM010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_332747"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /gene="crtI"
                     /locus_tag="AC16QF_10900"
     CDS             complement(<1..557)
                     /gene="crtI"
                     /locus_tag="AC16QF_10900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR6449107.1"
                     /translation="MNTSQRPTAAVIGSGFGGLALAIRLQSAGFRTTLFEGRDRPGGR
                     AYVYEDEGFTFDAGPTVITDPDCLRELFALSGRSLEDYLELTPIAPFYRLCWEDGYSF
                     DYSNDPDETAAQIGAKNPADVDGYRRFLAYSEEVLKEGYVKLGSVPFLDFASMIKAAP
                     QLVRLQSYRSVYSMVSRFVKDEQLR"
     gene            complement(554..1726)
                     /gene="crtY"
                     /locus_tag="AC16QF_10905"
     CDS             complement(554..1726)
                     /gene="crtY"
                     /locus_tag="AC16QF_10905"
                     /EC_number="5.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045436 - lycopene beta cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene beta-cyclase CrtY"
                     /protein_id="HIR6449108.1"
                     /translation="MHYDIIFVGGGLAGSLCAYRLRQLRPELGVLVLEQEARLGGNHT
                     WSFFASDLDEAELGWTEPFVAHQWDGYEVRFPGRRRRLTTGYRSSNSDRLHAVVSAAL
                     GDGVRCGAAVAAMDAGSVRLRDGETLTARCVVDARGPDRAPRLELGFQKFTGLILDLA
                     APHGLEVPIIMDATVDQGGDYRFVYVLPLGPSEVLVEDTRYSVTPGRDALSDESAVRG
                     YAQARGWQVQSVRGREEGVLPITLGGDIEGLWQDAAGMPLIGLRAALFHATTGYSFAD
                     AVRTADAIATSADLSSEALGGLIRRRSIRHWRGQSYLRFLNRMLFLAARPGQGYRVME
                     RFYGLPQGLIERFYADRLTSLDRLRILTGKPPVAILPALRCLSESSVKRFHSGIEF"
     gene            1819..2583
                     /locus_tag="AC16QF_10910"
     CDS             1819..2583
                     /locus_tag="AC16QF_10910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR6449109.1"
                     /translation="MLTFLALTAIIAFRYMLISGLFYWLLWRRKSSGAGAIKLMAGQP
                     AAGAVQKEIGWSLLSSFIYAAPAAVVFELWRLGGTAVYMDLAAMPLWYLPVSVGVYLF
                     LHDTYFYWTHRLMHQKRVFPIMHRVHHESRPPTPWASFSFHPYESVLGAIFLPALALV
                     IPIHIGAVLFILTVMTLAAVLNHSGYEIFPRWWLRGWPGHHLITAAHHDLHHKHFSCN
                     YGLYFRFWDKLMRTDIMESEYDFLAPSAEVPASPHR"
     gene            complement(2519..3859)
                     /locus_tag="AC16QF_10915"
     CDS             complement(2519..3859)
                     /locus_tag="AC16QF_10915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6449110.1"
                     /translation="MSGPVLTSSFGLRMSAFTALYLAQGVPIGLFSIALPAWLVEQGA
                     DAADIAALVAVTGLPWAFKLIAGPFMDRFSFPAMGRRRPWVMGAQAGFTVSMVSLALV
                     SGPSDGLWPIMVIGFVINTFAAVQDVAVDGMAIDVLPISERGRANAFMAFGQAAGFSG
                     FGALDGYLLSRFGLGVTAIVSAAAIGAVLVFVAVTREREGERLLPWTAGMATAREIAP
                     ARSMLEILRDILRVLLLPMSLLLIAVEFLARAGSGVGLAIMPVLAVDELGYASEVYAY
                     WLGLMGGLSAFVGIFCGPAIDRLGARKVLVVGLLGGSLVTVAYALAAGARGTAGFDLT
                     MLAAAQIFGQVFFVSMIAMFMGICWARVAATQFAIYMSLANLARSAGAGAFALVATDL
                     SVFDALYLVAALSVAAVLLLQRFDPAAHAARLALIDEERLAPRQTERESRTPTP"
     gene            complement(3856..>4123)
                     /locus_tag="AC16QF_10920"
     CDS             complement(3856..>4123)
                     /locus_tag="AC16QF_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449111.1"
                     /translation="AISQADTTRLARTASVRMSLASRQETPIDPAASVLEAGVRLGAV
                     TFYGLTSAARRFEPLLILSYQGISILEDAGQENKRRHDRGSVSL"
BASE COUNT          832 a         1386 c         1238 g          667 t
ORIGIN      
        1 tgccgcagct gctcgtcctt cacgaaccgc gacaccatgc tgtagacgct gcgatagctc
       61 tgcagccgaa ccagctgggg cgccgccttg atcatgctgg cgaagtcgag aaacggcacg
      121 ctgcccagct tgacgtatcc ctccttgagg acctcctccg agtaagcgag gaagcgccga
      181 tagccgtcaa cgtccgctgg gttcttggcg ccgatctgcg cagcggtctc atccggatcg
      241 ttgctgtagt cgaaggagta gccgtcctcc cagcagagcc ggtagaacgg agcgatggga
      301 gtcagctcca ggtaatcctc gagcgaccgg ccgctcaacg caaacagctc acgaaggcag
      361 tccgggtcgg taatgactgt gggccccgcg tcaaacgtga acccctcgtc ctcgtagacg
      421 taggcccggc cacccggacg gtcgcggccc tcgaagaggg tcgtgcggaa gccggccgat
      481 tgcaggcgta tggcgagcgc aagcccacca aagccggatc cgatgacggc ggctgtcggt
      541 cgctgggaag tgttcaaaac tcgatccccg agtgaaatcg cttcacgctg ctctccgaca
      601 ggcagcgcaa tgcaggcaga atggcaaccg ggggtttgcc agtcaatatc cgcaaacgat
      661 cgagactcgt gagacggtct gcgtagaacc gctcgatcag accttgcggc aacccgtaga
      721 atcgctccat cacgcgatat ccctggcccg gccgcgcagc gagaaacagc atgcgattga
      781 gaaacctcag ataagactgc ccacgccagt ggcggatcga ccggcgtcga atgaggccgc
      841 caagcgcttc ggaggataga tccgcggacg tggcgatggc gtctgccgtg cggacggcat
      901 cggcaaacga gtaccccgtg gtggcgtgga acagagcggc acgcaggccg atcaggggca
      961 tgccggcagc atcctgccaa agcccttcga tgtccccgcc aagggtaatc ggaagcacgc
     1021 cttcctcccg gcccctgacg ctttgcacct gccagccacg ggcttgcgcg tatcctcgca
     1081 cggcgctctc gtccgaaagg gcgtcccgac ccggagtcac gctgtagcgg gtgtcctcca
     1141 cgagcacctc cgacggaccc aggggaagga cgtaaacgaa ccggtagtcg cctccttgat
     1201 caaccgttgc atccatgatg atgggcacct ccagaccatg gggcgcggcg agatccagaa
     1261 tcaacccggt gaatttctga aacccgagtt ccaggcgcgg tgccctgtcc gggccacgag
     1321 catcgacaac gcaccgggcc gtgagggttt ctccatcccg gagcctgacc gaaccggcat
     1381 ccattgccgc caccgccgcc ccgcagcgaa ccccatcgcc gagcgcggca gacaccacgg
     1441 catggagccg atccgagttg gacgaccggt atccggtcgt gagcctgcgg cggcgtcccg
     1501 gaaagcggac ctcgtagccg tcccactgat gcgccacgaa aggttccgtc cagccaagct
     1561 cggcttcgtc gagatccgaa gcgaagaacg accaggtgtg gttaccgccg aggcgcgctt
     1621 cctgctccag gacgagcacg ccgagctccg gacgcagctg acgaagccgg taagcacata
     1681 ggctaccggc gagcccaccc ccgacgaata tgatgtcgta gtgcaatcgg cgagtccccg
     1741 ctaaacttcc gacattctag cgaagaagct cctcactccg cccaacccat gctggccgac
     1801 gttcccccgg aaaggctgtt gctcacgttc ctcgcgctga ccgcgatcat cgcgttcagg
     1861 tacatgctga tctccggtct gttctactgg ctgctgtggc gccgaaagtc atcgggcgca
     1921 ggggcgatca agctcatggc cggccagcca gctgccggcg cggtgcaaaa ggaaatcggc
     1981 tggtcactgc tgtcctcgtt catctacgct gcaccagcgg ccgtggtctt cgagctgtgg
     2041 cgcctcggcg gcaccgccgt ctacatggac ctggccgcca tgccgctttg gtatctgccc
     2101 gtgagcgtgg gggtctatct ctttctgcac gatacctatt tctactggac ccatcgtctc
     2161 atgcaccaaa agcgggtttt tccgatcatg caccgggtcc atcacgagtc caggccgccc
     2221 acgccctggg cgtcattctc gtttcacccc tacgagtccg ttctcggcgc catctttctg
     2281 cccgcgctcg ctctggtgat tcccattcac atcggtgccg ttctcttcat attgaccgtg
     2341 atgacactcg ctgccgtgct gaatcattcg ggatacgaga tctttccccg atggtggctg
     2401 cgtggctggc ccgggcacca cctgatcacg gctgcccacc atgaccttca tcacaagcac
     2461 ttcagctgca actatggtct ctacttccgg ttctgggaca agctgatgag aacggacatc
     2521 atggagtcgg agtacgactt tctcgctccg tctgccgagg tgccagcctc tcctcatcga
     2581 tgagcgccag ccgggcggcg tgagccgcag gatcgaatcg ctggagcagc aaaaccgcgg
     2641 cgacgctcag cgctgcgacc aggtagagcg catcgaatac gctgagatcc gtcgcgacga
     2701 gcgcaaacgc gcctgcgccc gccgacctgg ccaggttcgc cagcgacatg tagatggcaa
     2761 actgggtagc ggcgactctt gcccagcaga tgcccatgaa catggcgatc atcgacacga
     2821 agaagacctg accgaatatc tgggcagcgg ccagcatggt gagatcgaat ccggctgtac
     2881 ccctcgcccc cgccgccagc gcataagcaa ccgttaccag cgagccgccg agcaggccca
     2941 ccacgagcac cttccgggcg cccaggcgat cgatggcggg cccgcagaat atgccgacga
     3001 aggcgctcag cccgcccatc aatccaagcc agtaggcgta aacttccgat gcgtaaccca
     3061 gctcgtctac ggccagcacg ggcatgatgg ccaacccgac tcccgaaccc gcgcgcgcaa
     3121 gaaactccac ggcgatgaga agcaggctca tgggcagcag gagcacccgg agaatgtccc
     3181 tgagaatttc cagcatcgat cgggccggag cgatctcccg ggcagtcgcc atgcccgccg
     3241 tccagggcag cagacgctcc ccttcccgct cccgtgtcac cgcgacgaag actaagaccg
     3301 cgccgatggc tgcagccgat acgatggcgg tgacgcccag accaaaacgg ctgagcaggt
     3361 agccgtccag cgcaccgaat cccgagaagc ccgctgcctg gccgaacgcc atgaaagcgt
     3421 tcgcccggcc ccgctcgctg atcggcagca cgtcgatggc catgccgtcg acggccacgt
     3481 cctgaacggc ggcgaacgtg ttgatcacga agccgatcac catgatcggc cagaggccat
     3541 ccgaagggcc ggacaccagg gccagggaga ccatggatac ggtaaagccg gcctgggcac
     3601 ccatgaccca gggtcgacgc cgacccatgg cggggaaact gaagcggtcc atgaagggcc
     3661 cggcgatcag cttgaaggcc cagggaagcc cggtgacggc aacgagcgca gcgatgtccg
     3721 cagcatccgc gccctgctcc acgagccatg ccggaagagc gatcgagaac agaccgatgg
     3781 gtacgccctg cgccaggtaa agggcggtga atgccgacat gcgcagcccg aaggacgagg
     3841 tgagaaccgg cccgctcaca gggacacgct cccacgatcg tgacgacgct tattttcctg
     3901 acccgcgtct tccaatatgc tgattccctg gtaagacaag atgagcaacg gctcgaagcg
     3961 ccgcgctgcc gaggttaacc catagaacgt gacggctccc aaacggacac ccgcctctaa
     4021 aacggacgcg gccggatcta tcggagtttc ctggcgactg gccagcgaca tccggacgct
     4081 ggccgttctc gcaagcctcg tggtgtcagc ctggctgatc gcc
//
LOCUS       DBNHLM010000237         6036 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1231961, whole genome shotgun sequence.
ACCESSION   DBNHLM010000237 DBNHLM010000000
VERSION     DBNHLM010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6036)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1231961"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /locus_tag="AC16QF_10925"
     CDS             complement(<1..1312)
                     /locus_tag="AC16QF_10925"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6449112.1"
                     /translation="MLDDLFSKKPLSLAVASAIAVSQPMAVAAATGQIEELVVTATKR
                     AESTQDIPVSVQAITGEDMKEAGVATFEEYIVYLPNVVRSGRGPGKNDLYIRGAATEQ
                     AGVTVSSANGTTPAVALYLDEQPVSFGGRNLDIYAADLQRVEVLPGPQGTLFGVSSQA
                     GTVRLITNKPNHEQFEAGFNAALSSTKGGDLSNSVEAYINVPVTDRLALRFVGYNDHQ
                     GGWIDNVEGTFTPSIDTINRAAIAFSPTFRPKIDPNASLQSTSNDLLVEDDFNDATYS
                     GGRVSASFDINDDWNLLLQHTAQSLDVEGVFEYDPHLDGKDSVNRYTPNKNQDDFGLT
                     TWTVEGRLGMLDVIYTGGYLDREVDATIDYTGYNNGGGYVAYYLCNFGEASYDTCFDP
                     TKQYAGDTDNERVTHEFRVTTPADNRWRVTAGVFIDDQETNTVGQ"
     gene            1699..2679
                     /locus_tag="AC16QF_10930"
     CDS             1699..2679
                     /locus_tag="AC16QF_10930"
                     /inference="COORDINATES: protein motif:HMM:NF016974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HIR6449113.1"
                     /translation="MELSHPFIRLPFRFDADRLASELADIDDAAWLPHPSGMPGNSAV
                     ALVSRDGGDNDEFQGTMQPTGHLRRSPYMAQVMGSFGEILARSRLMRLSSKCEVQMHV
                     DFNYHWYTRVRIHIPIVTDPRLTFCCADERVHMAPGECWIFDSWRRHRVINASDSDRV
                     HLVVDMSGSSRFWRMVRHVQGSPDHAEEEFVAFDPGMQVSLSTERYNTAPVMAPGEVD
                     ALVKELVVDFSSHPDNDPGLVRRYALLLGDFCMDWRQIWLQHGYEREGIASYAALIDG
                     VQRQLHPNPRALVTSSNQVGVNPIIVQRILRPALAPEHLDDYLHDVRANG"
     gene            2749..3228
                     /locus_tag="AC16QF_10935"
     CDS             2749..3228
                     /locus_tag="AC16QF_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6449114.1"
                     /translation="MSEEEQVLITIRKIIRATDIYSRQLSKTVGLTVPQLLVLQAIRD
                     LGAVAISKISSEISLSQATVTSIIDRLEVRGLVSRRRSEADKRVVHAFLTEPGAELLD
                     SAPTPLQEVFRSRFDALEAWEKSMIIASLQRVAAMMDAEQLDASPMLAVGSPDPAAS"
     gene            complement(3225..3836)
                     /locus_tag="AC16QF_10940"
     CDS             complement(3225..3836)
                     /locus_tag="AC16QF_10940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6449115.1"
                     /translation="MGLYAKHVLPRMIDVLCGQPPMTELRRRYVPQASGDVLEIGIGS
                     GHNLAHYSSRVSSVTGLDPAAELTVMAQERANGLHTDVKLLQVSGEEIPADDARYDSL
                     VCTWTLCSIPNVYAAVREMRRVLKPGGRLYFVEHGASPDLPVARWQRRIEPVWKKIGG
                     GCHLTRRADELLQDAGFKLAELDTGYLPGPRFAAFMIHGIAER"
     gene            complement(3846..5411)
                     /locus_tag="AC16QF_10945"
     CDS             complement(3846..5411)
                     /locus_tag="AC16QF_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6449116.1"
                     /translation="MTHFPQRIAADRGDAPALTDERGSTSWAELNARSNQLVHAFRAM
                     GLVPGDAIAIYAGNCREYYEVMMAANHAGLIYVPVNWHFSPEELAYVIDNAGARLLVT
                     EGQFLANAEAAVARGETPGLEQCVVIRPEADTTAFTDYEAFIGDQSTAEPDDQGAGGP
                     MFYTSGTTGRPKGVKSSTFAPGGPLAVLEMIGSGLSQMLQIPTDGTTYICGPVYHSAQ
                     WAFSFLPLISGSHVVMRHRFDPAESLQVMDAHEVTNVHLVPTQFHRLLRLSDDERAGF
                     SGSSLQVVWHGAAPCPPDVKRRMIQWWGPVVAEYYGSTEGSIVTTASASQWLERPGTV
                     GKPSPLVEVTIRDDDGQPLPASESGQIYVKSLMGSDFEYHNEPAKTAEVHLEPGTYTF
                     GDIGYLDDDGYLYLSDRKIDMIISGGVNIYPAEIEAVLVNHPAVEDAAVFGIPNEEFG
                     EEVKAAIKLRPGQSGNEELSADIIGFCRDHLAHYKAPKSIDYEDEFPRHETGKLYKRL
                     LRDRYWQDADRKI"
     gene            complement(5428..>6036)
                     /locus_tag="AC16QF_10950"
     CDS             complement(5428..>6036)
                     /locus_tag="AC16QF_10950"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6449117.1"
                     /translation="NGNGWHTDVSCDEQPPMATMLQLHVLPATGGDTLFASMYGAYET
                     LSAAMRQFLAGLTATHASEHVYRGRYEDRGVEDAGRTYPSAVHPVVRTHPRSGRQALY
                     VNPSFTTRINELSGQESRTLLKMLYRHQQRAEFQVRFQWTPNAIALWDNRCTQHFALW
                     DYWPAERKGHRVTIGGERPFYEPGAEKPQAAGLRLASELSSQ"
BASE COUNT          983 a         1839 c         2031 g         1183 t
ORIGIN      
        1 actggccgac ggtgttggtt tcctgatcgt cgatgaagac gccggcggtc acgcgccagc
       61 ggttgtctgc aggtgtggtg acccggaact cgtgggtgac gcgttcgttg tccgtgtctc
      121 ccgcgtactg cttggtgggg tcgaaacagg tgtcgtagct cgcttcgccg aagttgcaca
      181 ggtagtaggc gacgtagccg ccgccgttgt tgtagccggt gtagtcgatg gtggcgtcca
      241 cctcgcggtc gaggtaaccg cccgtgtaga tgacgtccag catgcccagg cgaccctcta
      301 cggtccaggt ggtgagcccg aagtcgtcct ggttcttgtt gggcgtgtaa cggttgacgg
      361 agtctttgcc gtccagatgc gggtcgtact cgaacacccc ttccacgtcc agggactggg
      421 ccgtgtgctg cagcagcagg ttccagtcgt cgttgatgtc gaacgaggcg ctcacccggc
      481 cgccggagta ggtggcgtcg ttgaagtcgt cctcgacgag gaggtcgtta ctggtggact
      541 ggaggctggc gttggggtct attttaggac ggaaagtggg agaaaaagcg atggcggcgc
      601 ggtttatggt gtcgatgctg ggcgtgaagg tgccctcgac gttgtcgatc cagccgccct
      661 gatggtcgtt gtagccgacg aagcgcaggg ccaggcggtc ggtgaccgga acgttgatgt
      721 aggcctccac ggagttgctc aggtcgccgc ccttggtgct ggacagcgcg gcgttgaagc
      781 cggcctcgaa ctgctcgtgg ttgggcttgt tggtgatcag gcgcacggtg cctgcctggg
      841 agctgacccc gaacagcgtc ccctggggcc ctggcagaac ctccacccgc tgcaggtcgg
      901 cggcgtagat gtccagattt ctgccgccga aggacaccgg ctgctcgtcc agatacaggg
      961 ccaccgcggg cgtcgttccg ttggcggagg acacggtaac cccggcctgc tccgtggcgg
     1021 cgccgcggat gtacaggtcg ttcttgccgg gcccgcggcc gctgcgcacc acgttgggca
     1081 ggtagacgat gtactcctcg aacgtcgcaa caccggcctc cttcatgtcc tcgccggtga
     1141 tggcctggac ggataccggg atgtcctgag tggactcggc gcgtttggtg gcggtgacca
     1201 cgagctcttc gatctggccg gtcgccgcgg caaccgccat gggctgagat accgcgattg
     1261 cggaggctac ggccagtgaa aggggtttct tcgaaaatag atcatcgagc atgtacatgt
     1321 ctctccagta cgctgagtgg gcctgcgtcg cgtcgtcaat acggcttgct atggtggtcg
     1381 cgctcgttaa aggaacgcgg accgggcgcc tatacggctg gtatcgacgc caaatcgatg
     1441 tgagttaaat agattagggc cctaagtgtc tatgttactc aaatccgacg agaataccca
     1501 ttatttctca attattgggg tttaaggccg cgtgactgca gatctatata caaatgaatc
     1561 gaaataatta tcagagtttg tcgtctgttt tgtcggaaag ttggtaggct gcgaagcaaa
     1621 tttcaggtac ggcctcagac aaggaaggct cgcttgccgg actcggcgcg gttcgacccg
     1681 gacgggattc gttgatccat ggaactctca cacccattca tccggctgcc attcagattc
     1741 gacgcggaca ggctcgcctc cgagttggcc gacatcgacg atgccgcatg gttgccccac
     1801 ccttctggaa tgcccggcaa ttcagccgtc gcgctcgttt cccgcgacgg gggtgacaac
     1861 gacgagttcc agggcacgat gcagccgacg gggcatctgc gtcggtctcc ctacatggcg
     1921 caggtcatgg gatctttcgg cgagatcctc gcccgatcac gcctgatgcg tctgtcctcg
     1981 aaatgcgaag tgcagatgca cgtcgatttc aactatcact ggtacacgcg ggtccggatt
     2041 cacatcccca tcgttaccga tccgcggttg accttctgtt gtgctgatga acgcgttcac
     2101 atggcgcccg gcgagtgctg gatattcgat tcgtggcgcc gacatcgggt gatcaacgcc
     2161 agcgactccg accgcgttca cctcgtggtg gatatgtctg gttcctcgcg gttctggcgg
     2221 atggtccgcc atgtccaggg ctcgccggat cacgccgagg aagagttcgt tgctttcgat
     2281 cctggcatgc aggtctcatt gtccacggag cgttacaaca ccgcgccggt catggcccca
     2341 ggcgaggtcg acgccctggt caaggaactc gtggtcgatt tttcgtcgca tcccgacaac
     2401 gatccggggc tggttcgacg ctacgcgctg ctcctcggcg atttctgcat ggactggcgg
     2461 cagatctggc ttcagcacgg ctacgagcgg gaaggaattg ccagctacgc ggcgctgatc
     2521 gacggcgtgc agcgccagtt gcacccgaac ccccgggcgc tggtcacgag cagcaatcag
     2581 gtgggggtca atcccatcat cgttcagcgg attctgcgtc cggcgctggc accggagcat
     2641 ctcgacgatt acctccacga tgtgcgggcg aacggataac caaagctcgc cgtggcaggc
     2701 gggcgttggt aaggtttcgc tgtccatcag tcgtggcccg ggttggagat gagcgaagaa
     2761 gaacaggttc tgataacgat ccggaagatc atccgggcaa ccgacatcta ctcccgacag
     2821 ctgagcaaga ctgttgggct caccgtcccg cagttgctgg tcctgcaggc gataagggac
     2881 ctcggtgccg tggcgatttc gaagatatcc agtgagatca gcctgagtca ggccaccgtc
     2941 acctctatca tcgaccggct tgaagtccga ggtctggtct cgcggcggcg cagcgaggcg
     3001 gacaagcggg tcgtgcacgc tttcctgacg gagcccgggg ccgagctgct cgattccgcg
     3061 ccgacgccgt tgcaggaagt ctttcgaagc cgattcgatg ctctcgaagc ctgggagaaa
     3121 tccatgatca tcgcgtcgct gcagcgggtg gcggccatga tggacgccga gcagctcgac
     3181 gcatcaccca tgctggccgt tggcagccct gaccccgcgg cgagctagcg ctcggcgatc
     3241 ccgtggatca tgaacgccgc gaagcgcggg cccggaaggt agccggtgtc gagctcggcc
     3301 agcttgaagc cggcgtcctg aagcagctca tcggcgcgtc gcgtcaggtg gcagccaccg
     3361 ccgatcttct tccagaccgg ctcgatgcgt cgctgccagc gggcaaccgg caggtcgggc
     3421 gaggcgccgt gctcgacgaa gtacaggcgc ccgcctggct tcagcacgcg acgcatctcg
     3481 cgcacggccg cgtagacgtt ggggatcgag cacagggtcc aggtgcacac cagggagtcg
     3541 tagcgcgcgt cgtcggccgg tatctcctcg cctgagacct gcagaagctt gacgtccgtg
     3601 tgcagcccgt tggctctctc ctgagccatg accgtcagct cggcggccgg atccaggccc
     3661 gttaccgagg acaccctgga cgagtagtgc gccagattgt ggcccgatcc gatgccgatc
     3721 tcgagcacgt cgccgctggc ctggggcacg tagcggcgcc tgagctccgt catgggcggc
     3781 tggccgcaga gaacgtcgat catccgcggg agaacgtgct tggcgtagag tcccatggtc
     3841 gttcctcaga tcttccggtc cgcgtcctgc cagtagcggt cgcgcaggag ccgcttgtag
     3901 agcttacccg tctcgtggcg cggaaactcg tcctcgtagt cgatggattt cggcgctttg
     3961 tagtgggcga ggtggtcccg gcagaagccg atgatgtcgg cgctgagctc ctcgttgccg
     4021 ctctgtcccg ggcgcagctt gatggcggcc ttgacctcct cgccgaactc ctcgttgggg
     4081 atgccgaaca cggccgcgtc ttccaccgcg ggatgattga ccagcacggc ctcgatttcc
     4141 gcggggtaga tgttgacccc gcccgagatg atcatgtcga tcttgcggtc gctcaagtag
     4201 aggtagccgt cgtcgtccag gtagccgatg tcaccgaagg tgtaggtgcc tggctccaga
     4261 tgcacctcgg cggtcttggc cggctcgttg tggtactcga agtcggagcc catcaggctt
     4321 ttgacgtaga tctgaccgga ctcgctggcc ggcaggggct ggccgtcgtc gtcgcggatg
     4381 gtcacctcca ccagtgggga cggcttgccc acggtgcccg gccgctccag ccactgcgaa
     4441 gcgctcgccg tggtcacgat ggagccctcg gtggagccgt aatactcggc gaccacgggc
     4501 ccccaccact gaatcatgcg tcgcttgacg tcgggcgggc agggcgccgc gccgtgccag
     4561 actacctgca ggctcgatcc cgagaagccg gcgcgctcgt cgtcgctcag gcgcagcagc
     4621 cggtggaact gggtcggaac gagatgtacg ttggtcacct cgtgggcgtc catcacctgc
     4681 aggctctcgg ccgggtcgaa gcggtggcgc atgaccacgt gactgccgct gatcagcggc
     4741 agaaaggaaa acgcccactg cgcggagtgg tagacgggcc cgcagatgta ggtggtcccg
     4801 tccgtgggga tctgcagcat ctgcgacagg cccgagccga tcatctccag gacggccagt
     4861 ggcccgccgg gggcgaaggt gctcgacttc acgcccttgg gtcgcccggt ggtccccgag
     4921 gtgtagaaca tgggtccgcc ggcgccctgg tcgtcgggct ccgccgtgct ctggtcgccg
     4981 atgaacgcct cgtagtcggt aaacgccgtg gtgtctgcct cgggccggat gaccacgcac
     5041 tgctcgagcc ccggcgtctc gcccctggcc accgcggctt cggcgttggc gagaaactgc
     5101 ccctcggtca cgagcaggcg cgcgccggcg ttgtcgatca cgtaggcaag ctcctccgga
     5161 gaaaagtgcc agttcacggg cacgtagatc aatccggcat ggttggcggc catcatcacc
     5221 tcgtagtact cgcggcagtt gccggcgtag atggctatgg cgtcgcccgg gacgagcccc
     5281 atggcgcgga aggcatgaac gagctggttg ctccgggcgt tcagctcggc ccaggacgtg
     5341 ctgccccgtt cgtcggtgag cgccggggcg tctccccggt cggcggcgat tcgttgcgga
     5401 aagtgcgtca tcggtcggtt ccgtggttca ttgggaggaa agttcgctgg cgagccggag
     5461 cccggccgcc tggggctttt cagccccggg ttcgtagaag gggcgctcgc cgcctatggt
     5521 gacccggtgt cccttgcgct cggcgggcca gtagtcccag agcgcgaaat gctgcgtgca
     5581 gcgattgtcc cacagggcga tggcgttcgg cgtccactga aagcgcacct ggaactcggc
     5641 ccgctgctgg tgccggtaca gcatcttcag aagcgtgcgg ctctcctgac cggacagctc
     5701 gttgatccgc gttgtgaagc taggattcac gtagagcgcc tggcggccgc tcctcggatg
     5761 cgtgcgcacg acgggatgaa cggcgctggg gtaggttctg ccggcatcct cgacgccccg
     5821 gtcctcatag cggccgcggt agacgtgctc cgaagcgtga gttgccgtga gcccggcgag
     5881 aaactgccgc atcgccgcag acagggtctc gtacgcgccg tacatgctgg cgaacagcgt
     5941 gtcgccgccg gtggccggca gcacgtgaag ctgcagcatc gttgccatgg gcggttgctc
     6001 gtcgcaggac acgtcagtgt gccagccgtt gccgtt
//
LOCUS       DBNHLM010000238         9692 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1289961, whole genome shotgun sequence.
ACCESSION   DBNHLM010000238 DBNHLM010000000
VERSION     DBNHLM010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9692)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9692)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9692
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1289961"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="AC16QF_10955"
     CDS             complement(<1..266)
                     /locus_tag="AC16QF_10955"
                     /inference="COORDINATES: protein motif:HMM:NF016382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HIR6449118.1"
                     /translation="MQNRLRDRVRIVPGPKPRRIVGLDCAFAENDVLAVAVVWDVVGG
                     RVLETRGARRRVDFPYVPGLLSFREVPVMLDVLRRLTTSFDAVM"
     gene            449..2944
                     /locus_tag="AC16QF_10960"
     CDS             449..2944
                     /locus_tag="AC16QF_10960"
                     /inference="COORDINATES: protein motif:HMM:NF025761.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HIR6449119.1"
                     /translation="MKQEPLTEPYQRRQLADLPPSGVAYDEVLDGRELRHHWQFLDGT
                     FTATDSRSPEALNRTVQQILDESAVTYLEDGAQRPWRLDGLPYVLDPNEWPTLTRGLE
                     QRARLLNAVVADLYGPQTLLHRGLPLGLAFANPDYLLPACGYSAPEAVFLHLLAFDLG
                     RSPDGQWRVLSSRSEAPSGLGYTLENRLIMARSVPELFESGGIARIAQFFRAHSEYLH
                     ELGETTGRSDGLSVILSGGPGQESYFEHTFLGRYLGYPVVEGADLTVRDGEVFLKTLE
                     GLKPVSLIVRQVESSDCDPLELRAHSLQGVPGLLRAASTGRVAVANAIGSGLVENEAF
                     ASFLPSLCKQMLSEPLELPDLTTWWCGQPAELEHVLANRGELVLRNAFHRKPLLASSV
                     GAYMASDILPTGNASIPAKISRLPYQFVGRERIELSTVPYWTGADGWARAPMTLRLYV
                     GATGDGYKVLPGGLARLATPEGDISKDVWIPAAGAEWPATPPARTVATRRSDRDLSSR
                     TGDDLFWLGRYLERTEGAVRLYRALFSYAGGEGAASGPPVELGQLTRLLTTMDYLSPA
                     RARRAAAAGRGAFDQELWQILFDPESEDGLANVLGNVRRTAEHVRERLSRDAWRLFEG
                     LSAIPELRWRVHSIADALRLLDQLIEKLSAVNGQVHENMTRGYGWRLLDMGRRLERCT
                     YIVRVVKDLCTREPQQIGALNLLLDVCDSGLTHRFRYQTSPTLATVLDLLLVDDTNPR
                     SVAYQIGMLREHMATMPLEQRDGSLSESGRILLAAQSELALADTVKLAEVISRKGLRT
                     HLNRLLGRLEESMNGLHEVVTRTYFDHTISHWQ"
     gene            2946..3845
                     /locus_tag="AC16QF_10965"
     CDS             2946..3845
                     /locus_tag="AC16QF_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HIR6449120.1"
                     /translation="MTRYQIVHKTHYEYARPVSSSYQHLHLTPRSFAKQNVISSDLHI
                     EPAPTSNQTRQDYFGNPVTDVVIRSRHDSLEIISRALLDVNADENILLDLSPPWEAVA
                     GAVGDPRSSEAWDAARFCFPSPHVDPREALAYAEPSLSPGTPFLRSVEHITARIYEEF
                     TYKGGVTDVQTPVEEVLQSRAGVCQDFAHLAIASVRAFGLPARYVSGYLLTRPPQGQP
                     RLTGADASHAWISVWCPEFGWIDFDPTNNLRPRDEHITLGWGRDYSDVSPTRGFIHGG
                     GSQQLEVSVDVTPVPLADHRARR"
     gene            3855..5342
                     /locus_tag="AC16QF_10970"
     CDS             3855..5342
                     /locus_tag="AC16QF_10970"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HIR6449121.1"
                     /translation="MSQPNIAQTRTGAVEGRMKDGTLLFSGIPYAAAPEGSLRFRPAA
                     PVTPWTGVRPALKFGPAAPQVATGGLTNSAAVRWSEDCLSLNVCTPALDGALRPVLVW
                     IHGGSFRTGQGAVPWYNGNRFALHGDMVVVSINYRLGALGFSELSRLGSGFETSGVNG
                     LLDQIRALEWVRDNIQQFGGDPDRVTIAGESAGAFSVCSLLGSPLAQGLFHRAIAQSG
                     AAHHTLRKATAERITDLFLESLGIDDPTDLDSLPVERILDAQTEIVQRLEDRSGALAV
                     LGELHDPVGPFYPCVGNAVLPQSPIEAIRSGSGASIPLLIGTNADEATLWGYGDVPEQ
                     RLHEVVQAYNASAPLETLRAQRPKAATDQLMIALTTDFLFRIPAIRVAEARAGFDTAT
                     WMYLFSWKSRAFGGRLGATHALEIPFVFDNLDRAGVDIFLGEGPTPQALADVMHRAWI
                     RFVQGDDPGWERYEPGSRATMRFDDPCELIYDPDREEREAWDGYR"
     gene            complement(5343..7028)
                     /locus_tag="AC16QF_10975"
     CDS             complement(5343..7028)
                     /locus_tag="AC16QF_10975"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR6449122.1"
                     /translation="MRKDKGNVTPLFGERRRPPESPRPQVVVLDSADFVDAGELGREL
                     DSGADLDRPRLVLLERNELAVLRPHLEADPNLEFILKPVDQDTLQARIFKLVATSEAF
                     RDRQQQLSRVQDHVDRLAYYDRITDLPNNEFFRRHVDVQIRHARRFTRQLAVLAVDLQ
                     SFERVRRRAGEAGTEALMSLSARRIIEAIRDYDVVGQSPNVLPFGDERTMARMEGDRF
                     LLLLPELRHIEEAASVAERVVAAVEVPIQIGGAAFITRPKVGISVYPTDGDDSTSLIR
                     CAQRALLSSSDLRGGRVHFRCEAMNDVIIDRLINEAALRRAFENGGFHLVYQPKVELS
                     TGRTTGVEALLRLVDPELGELPPEEIVPLAEQVGLISELTRWVLAEVCGQIRRWRQAG
                     LPRVPVSVNVSATDMLTPDFADFLTGLLLDNGVAPQALELEIPCRSFDGANDGMAAMV
                     AELRTVGVTIAVGDFGADGSALDCLDHLPVDALKIDRHLIREITRSRISTAITAGAIA
                     ASRSLNLKVVAAGVETVEQVRLLRELDCLEVQGAAYSMPVSPEQLADWLIRTR"
     gene            7515..9362
                     /locus_tag="AC16QF_10980"
     CDS             7515..9362
                     /locus_tag="AC16QF_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR6449123.1"
                     /translation="MTTSNLLDGFTVGDFQVFPREEKIVGGDSSHHVEPKVMQVLVTL
                     ASNAGHTVSRDQILEEVWSDAVVGDEVLSRAISLLRGYFGDERTSPRYIRTVPRKGYE
                     LIAEVGAPPAKSTGAISRGRAYALVALVAALVVATAAVVRWWPATVDADLTLAVMPLA
                     ISGEASRLGYVADGLADHLITHLTRSPELHVVARRSSFLIRDTDANVRAIGEQLGARY
                     IVEGSLAERGANLLVTMYVVDTELGTNIWTTQLLGPANDLASLQVQAERAINEALRAR
                     LGVKLRTSRSAAGAISEDAYLKYHEARYQWSLRGELRIARAIALLQEAIDLAPDFAEA
                     HMALAQTLAVQPFYTDDPVAERFVKARASAARALELDASLAAEASALEGFLLLRERRW
                     QQAEIQLKKALRLDPDQVNAHYWYSWLLSALGKYDEAMREMQIAWRLNPVSAVLNDRL
                     AVAYLWVNDNAEAAARYQAAADLGYLESTQPESYILFLRRAGRFSEIAELLVRMGYSA
                     AWVEPFVRGLEHPDTRQEGAVAIEAASAAGDVPFELLFGIWVLFEDGDRAFANFDYGL
                     KTKYIEFLWADETEFLRDDARFADLIARLNLSDVINLDEIRSANQDRAK"
     gene            9419..>9692
                     /locus_tag="AC16QF_10985"
     CDS             9419..>9692
                     /locus_tag="AC16QF_10985"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HIR6449124.1"
                     /translation="MSGRDKPLLDLKGRPFVAWIAESVKDRVSQVIISANRNLQAYLE
                     VGEVLPDDGAGQGPLDGIATCARRCRTPFLFVCPGDAPHLDAAVIDR"
BASE COUNT         1756 a         3254 c         3013 g         1669 t
ORIGIN      
        1 cacatcacgg cgtcgaagga agtcgtcagg cggcgcagca cgtcgagcat caccggaact
       61 tcgcgaaagg aaaggagccc cggaacgtaa ggaaagtcga cccggcggcg cgcgccacgc
      121 gtctccaaga cgcgccctcc gacgacatcc cagaccaccg ctaccgcgag aacgtcgttt
      181 tccgcgaaag cgcaatccag cccgacgatt ctccgcggtt tcggtcccgg gacgattcgg
      241 acccgatccc gcagacgatt ctgcacggcc accgcggcgc gaggcgcgag acgccatctg
      301 tgcggggccc gaggaacgga attggggcga tcgtgccgag gcattacggc ttgcaggggc
      361 caagcccccc acctaacctt gtccggcccc ccactatgca cggcgggatc cggttcgcaa
      421 agccgacctt tctgagcgac aggccaactt gaagcaggaa cctctcacag aaccttacca
      481 gagaaggcaa ctggccgacc tgccaccctc gggcgttgcc tacgacgagg tgctggatgg
      541 cagggagctg cgccatcact ggcaatttct cgacggcacg ttcacggcga cggacagccg
      601 ctcgccggaa gccctcaacc ggacggttca gcagatcctc gacgaaagcg cggtgaccta
      661 cctggaagac ggcgctcagc gcccctggcg tctcgacggc ctgccatacg tcctggatcc
      721 caacgagtgg cccacgctaa cccgcggcct ggaacagcgg gcgcgtctgc ttaacgccgt
      781 cgtcgccgat ctgtacggtc cgcagacact gctgcaccgc ggcctgcccc tcggtcttgc
      841 ttttgccaac cccgactacc tgctgccggc gtgcggctac tccgcccccg aggccgtctt
      901 tctgcatctg ctcgcattcg atctgggacg atcacccgat gggcagtggc gggtgctgag
      961 cagccgcagc gaagcccctt ccggtctggg ctacacgctg gagaatcggc tgatcatggc
     1021 gcgatcggtg cccgagctgt tcgagtcggg cggcatcgcg cgaatcgcgc agttcttccg
     1081 cgcccacagc gagtacctgc acgagctggg agagacgacc ggacgcagcg acggtctttc
     1141 cgtcatcctc tccggaggtc ccggtcagga aagctacttc gagcacacct tcctcggccg
     1201 ctaccttggc tatcccgtgg tggagggagc cgatctgacc gtccgcgacg gcgaggtctt
     1261 cctgaaaact ctcgaagggc tgaagccggt cagcctgatc gtccggcagg tggagtcgtc
     1321 cgactgcgac ccgctggagc tgcgcgcgca ttccctgcag ggggtgccag gcctgcttcg
     1381 tgctgcgagc acggggcggg tggcggtggc aaacgccatc ggcagcggcc tggtggaaaa
     1441 cgaagcgttc gcgagtttcc tgccctcgct gtgcaagcag atgctgagcg agcccctcga
     1501 gctgccggat ctcaccacct ggtggtgcgg ccagccggcc gagctggaac acgtccttgc
     1561 caaccggggg gagctggtgc tgcgtaatgc tttccaccgc aagccgctgc tggccagcag
     1621 cgtgggcgca tatatggcca gcgacattct cccgactggc aacgcatcga ttccggcaaa
     1681 gatctcgcgc ctgccctacc agttcgtcgg gcgtgagcgc atcgagctgt ccaccgttcc
     1741 ctactggaca ggggcggacg ggtgggcacg ggcaccgatg acgctgcgtc tgtacgtagg
     1801 cgccaccggt gacggctaca aagtgttgcc cggcgggctt gcacgcctgg ccacacccga
     1861 aggagacatc agcaaggacg tctggattcc cgccgcgggg gccgagtggc cggcgacgcc
     1921 accggcgcgc acggtggcaa cccgccgcag cgaccgcgat ctatccagcc gcaccggtga
     1981 cgacctcttc tggctgggac gctacctgga gcgaaccgag ggcgccgtgc gcctttaccg
     2041 ggcgctgttc agctatgccg gtggcgaggg cgctgcaagt ggaccgcccg tcgagcttgg
     2101 ccagctgaca aggctgctca cgacgatgga ctacctctca ccggccagag cgagacgagc
     2161 ggccgcagcc ggccggggcg ccttcgacca ggaactgtgg cagatcctct ttgaccccga
     2221 gagcgaggac ggcctggcca acgttctcgg caacgttcgg cgaactgcgg agcacgttcg
     2281 cgaacgtctt tcgcgcgacg cgtggcgtct gttcgaaggg ctttcggcga taccggaact
     2341 gcgctggcgc gttcactcca tcgccgatgc gctccggctg ctggatcaac tgatcgagaa
     2401 gctgtcggcg gtcaatggcc aggtccacga aaacatgacc cgcggctacg gctggcggct
     2461 actggacatg ggtcgacggc tggagcgctg tacctacatc gtccgggtcg tcaaagatct
     2521 ctgtacccgg gaaccgcagc agatcggcgc actgaacctg ctgctcgacg tgtgcgacag
     2581 cgggctcacc caccgcttcc gataccagac gagccctacc cttgcgaccg tcctcgatct
     2641 gctgctcgtc gacgacacca acccacgctc cgtggcttac cagatcggca tgctgcgcga
     2701 gcacatggcg accatgccgc tggagcagcg ggacgggtcg ctgagcgagt caggccgcat
     2761 tctgctggcc gcccaaagcg agctcgctct ggcagacacc gtcaagctcg cggaggtgat
     2821 cagcaggaag gggctgcgta ctcatctcaa tcgtctgctc ggccggctgg aggaatccat
     2881 gaacggtctc cacgaagtgg tgacccgcac gtacttcgat cacaccatca gccactggca
     2941 gtgagatgac gcgttatcag atagttcaca agacgcacta cgagtacgct cgtccggtga
     3001 gctcgtccta ccagcatctg cacctcacgc cgcgcagctt tgccaagcag aacgtgatca
     3061 gctcggacct gcacatagag ccggccccca cttcgaacca gacgcggcag gattacttcg
     3121 gcaacccggt caccgacgtc gtcatccgca gcagacacga ctcgctcgag atcatcagtc
     3181 gtgcgctgct tgacgtgaat gcagatgaga acatccttct ggatctgagc ccgccgtggg
     3241 aagccgtcgc cggggcggtc ggcgatccca gatcgagcga ggcctgggat gccgcccgct
     3301 tctgctttcc ctcgcctcat gtcgatccgc gagaagctct tgcctacgcc gagcccagcc
     3361 tgtcgcccgg cacgccattt ctgcgatccg tcgagcacat cacggctcgg atctacgagg
     3421 agttcaccta caagggcggc gtcaccgacg tccagacgcc ggtggaagag gtgctgcagt
     3481 cgagggccgg ggtgtgccag gactttgctc acctggccat cgccagcgtg cgggcgttcg
     3541 ggctgccggc caggtacgtc agcggctacc tgcttacccg acctccccaa ggacagcctc
     3601 ggctcaccgg cgcggatgcg tcccacgcct ggatatcggt gtggtgcccg gagttcggct
     3661 ggatcgactt cgatccgacc aacaatctcc gcccaaggga cgagcacatc acgctgggct
     3721 ggggccgcga ctacagcgac gtcagcccca cccggggatt cattcacggc ggtggaagcc
     3781 agcagctgga agtttccgtg gacgtgacac ccgtcccgct ggccgatcac cgcgcccgcc
     3841 ggtaacctct tcccatgagc caaccaaaca ttgcccagac ccgaaccggt gcagtagaag
     3901 gccgcatgaa agacggcacg ctcctgtttt ccggaatacc ctacgccgct gcaccggagg
     3961 gttcccttcg tttcaggcct gcggcaccgg taacgccctg gaccggagtc cgcccggcgt
     4021 tgaagttcgg acccgccgct cctcaggtgg cgaccggcgg tctcaccaac agcgccgctg
     4081 ttcgctggtc cgaggattgc ctatccctca acgtctgcac cccggcgctg gacggagcgc
     4141 tgcgcccggt gctcgtgtgg attcacggcg gcagcttccg caccggccag ggagcggtgc
     4201 cctggtacaa cggcaaccgg ttcgcgcttc acggcgacat ggtcgtcgtc agcatcaact
     4261 accggctggg cgcgctggga ttctccgagc tgtcccgcct cggatcgggt ttcgagacct
     4321 caggggtgaa cggcctcctc gaccagatcc gagctctcga atgggtacgg gacaacatcc
     4381 agcaattcgg cggcgaccct gaccgggtca cgatagccgg tgagtctgcg ggcgctttca
     4441 gcgtctgctc gctgctcggc tcacctctcg cccagggact gtttcaccgg gcgattgcgc
     4501 aaagcggggc cgcccaccac accctgcgca aggcaactgc cgagcggatc accgacctct
     4561 ttctcgaatc gctgggcatc gacgacccga cagatctgga ctcgctgccg gtggagcgca
     4621 tcctcgatgc ccagaccgag atcgtgcagc ggctcgaaga tcgctccggc gctctcgccg
     4681 ttctggggga actccacgat cccgtgggac ccttctaccc ttgcgtgggt aacgccgttc
     4741 tgccacagag ccccatcgag gccatccgca gcggctccgg cgccagcatt ccgctgctca
     4801 ttggaaccaa tgccgacgag gctacgctgt ggggttacgg agacgttccc gagcaaaggc
     4861 ttcacgaggt cgtccaggcc tacaacgcct cggcgccgct ggaaactctc cgcgcccagc
     4921 ggcccaaagc ggcgacggat cagctgatga tcgctctgac caccgacttt cttttccgga
     4981 tccccgccat ccgggtggcc gaggcgcgag ccggcttcga caccgcgacg tggatgtatc
     5041 tcttctcctg gaaatcacgg gcgttcggcg gccgcctcgg agcgactcat gccctggaaa
     5101 tccccttcgt tttcgacaac ctggatcggg cgggcgtgga catattcctg ggcgaaggac
     5161 caacccccca ggcgctcgca gacgtcatgc atagagcctg gatacgcttc gtgcaggggg
     5221 acgatcccgg ttgggagcga tacgagccgg gctcccgggc aaccatgcgc ttcgacgatc
     5281 cctgcgagct gatctacgac cccgatcgtg aggaacgcga agcctgggac ggctatcgct
     5341 gatcagcggg tgcgaatcag ccagtcagcc agctgctcgg gcgaaactgg catggagtat
     5401 gcggctccct gaacctccag gcagtcgagc tcgcgcagaa ggcgcacctg ctccacggtt
     5461 tccacgcccg ccgccaccac cttgagattc aatgaccgcg aagccgcgat ggcaccagcg
     5521 gtgatcgccg tgctgatcct gcttctggtg atctcgcgaa tcaggtgtcg gtctatcttc
     5581 aaagcgtcca ccggaaggtg atccaggcaa tccaacgccg atccgtcggc gccaaagtcg
     5641 cccacggcga tggtcacacc gacagttctc agctcggcga ccatagccgc catcccgtca
     5701 ttcgccccgt cgaaactccg gcagggtatc tccaactcca gagcctgggg cgcgacgccg
     5761 ttgtcgagca gcaggccggt caggaagtcg gcgaaatcgg gcgtcagcat atccgtggcg
     5821 gacacgttga cggacacggg tactcgtggg agcccggcct gtcgccagcg tcgaatctgc
     5881 ccgcacacct cggcaagcac ccatcgggtg agctcgctga tcaggcccac ctgctccgca
     5941 agcgggacga tctcctccgg aggcagctcg cccagctccg ggtcgaccag gcgcagcaga
     6001 gcttccacgc cggtggtgcg gccggtcgag agttccacct tcggctggta caccaggtgg
     6061 aatccgccgt tttcgaatgc tcgacgcagc gccgcctcgt tgatcagccg gtcgatgatc
     6121 acgtcgttca tggcttcgca gcggaaatgg actcggccac cgcgcaaatc gctcgatgac
     6181 agcaacgcgc gctgggcaca gcggatgagc gatgtggagt cgtcgccgtc cgtcgggtaa
     6241 acgctgatcc cgaccttcgg tcgcgtgatg aacgccgcgc cgccgatctg gataggcact
     6301 tccacagcgg caaccacccg ttctgcgacg ctggcggctt cctcgatgtg gcgcagttcc
     6361 ggcagcagca gcagaaagcg atcgccttcc atgcgcgcca tggtgcgctc gtctccgaat
     6421 gggagcacgt tgggagactg acccacaacg tcgtagtccc ggatagcctc gatgatgcga
     6481 cgggcagaca gggacatcag ggcctcggta cccgcttcac cggcgcgtcg cctgactcgc
     6541 tcgaagctct gcagatccac cgcaagcacc gccagctgtc gcgtaaaacg ccgcgcgtgg
     6601 cgaatctgca catccacgtg acggcggaaa aactcgttgt tcggcaggtc ggtaatcctg
     6661 tcgtagtagg ccagccggtc cacgtgatcc tgcacccggc tcaactgctg ctggcggtcc
     6721 ctgaaggctt cggaggtggc caccagcttg aatatcctag cctgcaaggt gtcctggtct
     6781 accggcttga ggatgaattc cagattcggg tccgcttcca gatggggacg cagaacggcc
     6841 agttcgtttc gctcgagcaa taccagacgc gggcggtcga ggtccgcgcc gctgtcgagt
     6901 tcccggccga gttcgcccgc atccacaaag tcggcactgt ccaacacgac cacctggggc
     6961 ctgggcgatt ccggtggacg ccgcctctcc ccgaacagcg gagtcacgtt gcctttatct
     7021 tttctcatcc agtgctcact atcgcagacc cggtgcctgc cgcgaccccc tcccgttcgc
     7081 cgcggcaaca agcaccgaat tctgctttcc ccccaactcg aatccacgca gatgatcgag
     7141 tggcttttct ataatcttca gcgaggtcat cccgccgagt gtctggcccg atcagccttc
     7201 ctgcagcgca gctagactga cgacatttgg caatttcgtt gccataacgg ccgttttgta
     7261 aaccctaccc aattacctgt tagggtgata acagcgcggt gtaagcgaca tgtaacctcg
     7321 gggtgatcgc aggggcgctg gcgtgacaac aacgcggatg cgcgcagccc ccgaggacgg
     7381 tatcttcagg ctatgaacca gcacgagtcc ctaaagccgg gcaacgttgg cagatgcaac
     7441 cggcgtcgct gcccgcacgc cccgcacacc aacggcagtc gtcgatcgac ctcctcgtcc
     7501 atggacgccg cccaatgaca acaagcaatc tgctcgacgg ttttaccgtc ggcgacttcc
     7561 aggtttttcc aagggaggag aaaatcgtcg gcggcgattc cagccaccat gtcgagccga
     7621 aagtcatgca ggtgctcgtg acgctggcgt ccaatgccgg ccacaccgta tcccgtgacc
     7681 agatcttaga ggaagtctgg tcggacgccg tggtcggcga cgaggtgctt tcccgggcca
     7741 tatcgttgct gcggggctac ttcggcgacg agagaacgag cccgcgttac atccgtacgg
     7801 tcccacgcaa aggttacgag ctgattgccg aagtgggcgc gcccccagcg aagagcacgg
     7861 gcgcgatcag ccggggccga gcgtacgcgt tggtggccct ggtagccgca ctggtggtcg
     7921 ccaccgcagc cgtcgtgcgc tggtggccgg ccacggttga cgcggacctc accctggccg
     7981 tgatgcctct ggccatctca ggggaagcga gtcgattggg gtacgtggct gacggactgg
     8041 ccgaccacct gatcactcac ttgacccgat ccccagagct ccacgtcgtc gcgcgtcgat
     8101 cgtccttcct gatccgggac accgacgcaa acgtccgcgc catcggcgag cagctcggcg
     8161 cccgctacat cgttgaaggc tctctggccg agcgtggggc caatctgctg gtgacgatgt
     8221 acgtggtgga taccgaactc ggcaccaaca tctggaccac gcagctgctc ggcccggcga
     8281 acgatctggc gtcgctgcag gtccaggccg agcgggccat caacgaggct ctgcgggccc
     8341 gactcggcgt gaagcttcga acgagccggt cggcggccgg cgcgatctcc gaggacgcct
     8401 atctgaaata tcacgaagct cgctaccaat ggagcctgcg gggcgagctt cgaatagcca
     8461 gggcaatcgc cctgctccag gaggcaatcg acctggcccc cgatttcgcc gaggcgcaca
     8521 tggccctggc ccagaccctg gcggtacagc ctttctacac cgacgacccg gtggctgagc
     8581 ggttcgtgaa ggcccgggcg agcgcagcaa gggcgctcga gctggacgcc tccctggcgg
     8641 cagaagcctc ggcgctggaa gggttcctgc tcttgcgcga acgacgctgg cagcaagccg
     8701 agattcagct gaagaaagcg cttcgcctcg acccggatca ggtcaatgcc cactactggt
     8761 actcctggct gctgtccgcg ctcggcaagt acgatgaagc gatgcgcgaa atgcagatcg
     8821 catggcgttt gaatcccgta tcggccgtgc taaacgaccg actggccgtt gcgtacctat
     8881 gggtcaacga caacgcggaa gcagccgctc gatatcaggc cgccgccgat ctcggctacc
     8941 tggagagcac ccagcctgag agctacatcc tgtttcttcg tcgagccggc cgcttcagcg
     9001 aaatcgccga actgcttgtg cgaatgggat actcggcagc gtgggtggag ccgttcgttc
     9061 gcgggctcga acatcccgat acccgacagg aaggcgcggt ggccatagag gccgccagcg
     9121 cggcgggcga cgtacccttc gagcttctct tcggcatctg ggtgctgttc gaagatggcg
     9181 accgggcatt cgcaaacttc gactacggac taaagacgaa gtacatcgag tttctctggg
     9241 cggacgaaac cgagtttctt cgcgacgatg cccgctttgc cgacctgatc gcccgcctga
     9301 acctctccga cgtgatcaac ctcgacgaga ttcgaagcgc gaaccaagat cgcgcaaagt
     9361 gagcctgccc ccggaatcgc tgacggccct cgttctttgc ggcggcgcgg ggcgtcggat
     9421 gagcgggcgc gacaagccgc tcctggacct caagggcagg cccttcgtgg cgtggattgc
     9481 cgagagcgtc aaagatcgcg tgagccaggt gatcatcagc gccaacagaa atctgcaagc
     9541 ctacctcgaa gtcggggaag tgctcccaga cgacggcgcg ggtcagggcc ctctcgacgg
     9601 catagccacc tgcgcccggc gatgccgaac tccgttcctt ttcgtgtgtc ctggcgatgc
     9661 gccgcatctg gatgccgccg tgatcgatcg gc
//
LOCUS       DBNHLM010000239        11873 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_751701, whole genome shotgun sequence.
ACCESSION   DBNHLM010000239 DBNHLM010000000
VERSION     DBNHLM010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11873)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11873)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11873
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_751701"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /gene="rpsB"
                     /locus_tag="AC16QF_10990"
     CDS             complement(<1..241)
                     /gene="rpsB"
                     /locus_tag="AC16QF_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR6449125.1"
                     /translation="MNISMRDMLAAGVHFGHQTRFWNPKMAPFIFGSRNKIHIINLER
                     TVPAFNEALTELRNLASRRNKILFVGTKRAAAKVIR"
     gene            486..1277
                     /gene="map"
                     /locus_tag="AC16QF_10995"
     CDS             486..1277
                     /gene="map"
                     /locus_tag="AC16QF_10995"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR6449126.1"
                     /translation="MVAKLENEDDIARMRNAGRLAAEVLEMIGEHVRPGASTEDLNGI
                     CHRYIVEEIDCIPAPLNYSNGPGQPAFPKSICTSVNHVVCHGIPAAKKVLKSGDIVNI
                     DVTVIKDGFHGDTSKMFMVGKPSVLAERLVRVTQECLYRGIDAVRAGATLGDIGHAIQ
                     SHAEAQHFSVVREFCGHGIGRVFHDEPQVVHYGRPNTGMRLEEGMTFTIEPMINAGRR
                     HVKILPDHWTVVTKDHKLSAQWEHTILVTATGAEVLTRRSDESLP"
     gene            1297..3987
                     /gene="glnD"
                     /locus_tag="AC16QF_11000"
     CDS             1297..3987
                     /gene="glnD"
                     /locus_tag="AC16QF_11000"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HIR6449127.1"
                     /translation="MSTTDRAARSPGSDGNEGSRRFGNADPFLEELKVRLAQADERMA
                     QRYWDRADVDELIAERTTFFDGLLREVWDHALSEDAQKGLALFAVGGYGRREQFPYSD
                     IDLLIVGKRPASHREAISRFLQVLYDLNLEVGHSVRTVRDCKKEAQKDITVATAIFER
                     RFLFGSDALRRRLDRVLSSSRLWPAEAFFRAKRDEQAHRHRQYDNVEYGLEPNVKSSP
                     GGLRDLQTALWVCQRKFGTTDPEALEALGMLTPLEREWLTEGRRYLWWVRFGLHLIAG
                     RKDDHLQFEHQRALAQRLGFADTNARLGVERFMHLYYRHVLELRELNDILLQHFEEAI
                     LSSRGRPRIEPINERFQIHNDYIEATDPTVFERSPRAILEIFVIMANRRDISGVRAQT
                     IRLIRDSLHLIDDSFRADPENAALFMDLLKAPYTLVSQLTRMRRYGVLARYLPEFGRV
                     VGQMQHDLFHIYTVDAHTMMVIGNMRRFRYRASEERFPVACHCVKEIPKPELLYVSGL
                     YHDIGKGRGGDHSELGAGDVTEFCRRHGLNEADTDLVAWLVRQHLLMSSTAQRKDIYD
                     PQVIHDFATEVKSEMRLNYLYALTVADINATNPDLWNSWRATLLRQLYGETRKALRRG
                     LETPLDKATSVNACRESAHERLVAAGMTPEAIEHAWRVPGDEFFLRHSPRQVAEVTQL
                     IHGHDVRSEPLVTLLDLKGQVSQEGATEVLLYTTDRENLFAASVLALSQLDLSIYDAN
                     IHTSADGLCLNTYVVLTAEGKPLPREGDRRGQVVEKLTRSLADTSTLTGSGKRRLPRQ
                     LRQFTLPTEVSLTTPAGSSNSELTIIASDRPGLLATIGLLFAELGLVVISARIATLGE
                     RVEDVFEISSREGEPITDEEAVYLLENTLRQRLDRQVAAS"
     gene            3994..5190
                     /gene="dapC"
                     /locus_tag="AC16QF_11005"
     CDS             3994..5190
                     /gene="dapC"
                     /locus_tag="AC16QF_11005"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 -
                     succinyldiaminopimelate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HIR6449128.1"
                     /translation="MNANLEKLQAYPFERLADLLAGVRGNAEFPHIALSIGEPKHAPP
                     EFVINLLSDPDRLRAGLGAYPATRGIPELREAISGWLARRYGVRLDPDNQVLPVAGTR
                     EALFSFGLAALSGKADARVLMPNPFYQIYEGAALLGNGVPHYVNCDRANDYQPDFAGV
                     PDSVWSACEVVYLCSPGNPTGETIPEDTVLWLLEQADRHDFVVAADECYSEIYLDDSH
                     PPAGLLEIAHRAGRDDYARCVAFHSLSKRSSLPGLRSGFVAGDAAILAGYFKYRTYEG
                     CALPLHVQQASAAAWSDEAHVEANRALYRRKFETVGRVLDPHLDLRTPAGGFYHWLHI
                     GEDDTAFARQLFEHCNLTVLPGSFLGRAGGDSNPGGGHVRVAWVASEAQCVEAAERLA
                     GWLQNR"
     gene            5263..6276
                     /locus_tag="AC16QF_11010"
     CDS             5263..6276
                     /locus_tag="AC16QF_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012722589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HIR6449129.1"
                     /translation="MSGIFAFAVGTVRRNTAGAPLDFYFPAPVINPEPDLAAQIEGSF
                     SSGAHEVGPAELARFAKAVGDSAMARLLSSLAETDRAMTAVRLDADEPIDSSATAYLK
                     LHLLSHRMALPNTLNLDGIFAHLPNVAWTDRGPIDPEELAAAQLQARLAGGHIEVLAV
                     DKFPRMTNYIVPSGVRIADASRIRLGAYLGDGTTVMHEGQINFNAGALGPNMVEGRIS
                     QGVILGEHSDLGGSASTMGTLSGGGEITISIGKHCLIGANAGTGIPLGDRCTIEAGLY
                     ITAGTPVTLIDETGEDVRTMKARELAGGSDLLFIRNGRSGRIECRSNRKAIELNEQLH
                     AHN"
     gene            6284..7429
                     /gene="dapE"
                     /locus_tag="AC16QF_11015"
     CDS             6284..7429
                     /gene="dapE"
                     /locus_tag="AC16QF_11015"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003293424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HIR6449130.1"
                     /translation="MSEGSQNPVLELTVELMRRASVTPSDEGCQELIGQRLAAMGFVN
                     RPMNFGDVSNLWATHGEGGPLFVFAGHTDVVPAGPQDAWTNPPFEPTLHDGFLWGRGA
                     ADMKASLAAMVVATERFLAAHPRHRGTLAYLLTSDEEGPAVDGTRRVVETLQAERAQE
                     MDFCVVGEPSSAHLLGDTVRVGRRGSVNARLTVFGTQGHVAYPELAENPVHTLLPALA
                     ELTNKRWDEGNDGFPPTGLQVSNIHAGTGAPNVIPGSLVVDFNIRFNTEQTSSGLHRQ
                     AAEILDRHGLQYDLEWVVSGEPFLTAPGDLRAAVNKAVGDVTGRTPEASTSGGTSDGR
                     FIAPTGCEVVELGPVNATIHKIDERVATADLLPLADIYQRVLDQLLG"
     gene            7432..8151
                     /gene="gspN"
                     /locus_tag="AC16QF_11020"
     CDS             7432..8151
                     /gene="gspN"
                     /locus_tag="AC16QF_11020"
                     /inference="COORDINATES: protein motif:HMM:NF013376.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HIR6449131.1"
                     /translation="MRTLILLGFLLFLTFCIVFAPAGLTGLVLDRVNGVDLTRVNGTL
                     WQGGGDLYVRSEPVGRLQWDVHPATVVAGALGCDFTLSHAHHQLRGTAVVGLNRTFTL
                     DVSGKVGAQTVNGWLEPYDMTISGTMDLSNVLLTFDDVALRDANGVVEWEGGSISYPA
                     GNGIQSSILPPLRATLGPGPAATITTLDDPAAALVTAELLDNGIAKVGVTARMTRLLN
                     LSWTETEPDSAVVLEVEEQVF"
     gene            8163..9101
                     /locus_tag="AC16QF_11025"
     CDS             8163..9101
                     /locus_tag="AC16QF_11025"
                     /inference="COORDINATES: protein motif:HMM:NF022800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HIR6449132.1"
                     /translation="MAALLNFIVTRLAEPARWLVVALIAYTLATTVLYLVSAPQSDVG
                     AGSDAKAPQGQPGRAGTGQQGPVDINRIVARNLFGVADASAPAATQQTAMAVATRLPL
                     ELQGVFVADASEDSAAIVAQKGRPGMLYVVGQKMPGNAELVEVHADHIVLRRAGVLET
                     LKFPTSIGQFVAAPDDLEDLADQALPFDMPTDLQEDADDDRDERGRKRPTSEALAEYR
                     ERIEADPQGVLADLGISAVNEGDASGYRLDDLNDSPFLSQTGLQPGDVILSVNGRPVG
                     DINQDQQELDNMLAQGSARLEVQRGTRRFFVTASLP"
     gene            9119..11023
                     /gene="gspD"
                     /locus_tag="AC16QF_11030"
     CDS             9119..11023
                     /gene="gspD"
                     /locus_tag="AC16QF_11030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HIR6449133.1"
                     /translation="MNKIFATTLTYAMVLLLLLPTSLPAQGQEQTWKINIKNADIQEF
                     VAQVAAITGKTFVIDPRLKGNVTVISNTSMDEEAVYALFLSVLRVHNFTATPSGDVIR
                     IQQNATGKQTPGVSGELGEVPPEELVTRVVSAQNVESAELVKILRPLIPQYGHIAAVA
                     VPNVVIISDHADNILRLMRMIEQIDVADEEEIVVVPLKEAWVGTIVALLEKVAPDQMG
                     RNAQGPQRIQIIANERNNSLVLKGKPRPVAEVLKIIEKLDQPATTAGATQVIYLKHAD
                     AVKVSEILNSVIEDRSRGEDGATERPNIQADETLNAIVVRADPGTMNEILEILESLDV
                     RRTQVLIEAAVVEVTITNASDIGVEMAIVDESGQTVPLASTSLDGAINSLIGNIIGDD
                     GEIDPVSGLASITSPTLAAARIDRGDVSFGAIVRALATNSNANLLSTPSILTLDNQEA
                     KIVVGNEVPFRTGSFTTSGNDSSNPFTTVQREDVGLQLTVTPHVHEGTSVRLEVAQEI
                     TNVINAPIGNDGFSDVVTSKRSIETTILAEDRQTVVLGGLIQDDITDTVKKVPLLGDI
                     PGLGALFRSNSKSRSKSNLLVFLRPTVIRNKEDSDTATNRKYTDIWEVEISSGGGKSD
                     ITDNFRGRKD"
     gene            11055..>11873
                     /locus_tag="AC16QF_11035"
     CDS             11055..>11873
                     /locus_tag="AC16QF_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449134.1"
                     /translation="MRQLRRLGYLVSRWLIRSIIRPRLIGDPPDPPREGRPVYLYSLE
                     NRSLSDLIVLDLLNEANHLPGPLDPVTLGSHTEGRRVFFLNRASYGLFQRNVMSTPSE
                     RLIRVLTHAGEANDAVIVPVTVLWGRAPSRQGSLFRSLLAEDWAVTTRLKRILNLVVS
                     RKEIYVHYGTPISIGELAEDDLDVNRMVRRAARLLRVQFRNQRVSAIGPDFSHQRTLV
                     NQILRSRAVTDAIAAADPEEKPERMERRARKYAMSIVSNMSYPTIRVLERLLTWF"
BASE COUNT         2276 a         3944 c         3670 g         1983 t
ORIGIN      
        1 cccggatcac cttggcggcc gcacgcttgg tgccaacgaa aaggatcttg ttgcgacggc
       61 tggcgaggtt tcggagctcc gtcagcgcct cgttgaacgc cgggacggtt ctctccagat
      121 tgatgatgtg gatcttgtta cgggacccga agatgaaggg agccatcttg gggttccaga
      181 aacgggtctg atggccgaaa tgcacgccag cggccaacat gtcacgcatg ctgatattca
      241 tatcctgtac tccgggttgc gcctccgcca tccccatgcc tcaacccgat cgaacgggca
      301 cccggagaca cgtgtcggac gacgtgagtc atttgtgtca aagtgacgtt tagtcgcctg
      361 gagcccgccg cccccctccc gcagatattt cttccgaagt tgggcggctt gcgttgcccg
      421 caggcggcgc gctttataac ataatcgccc caccgcttaa acaagcccaa gccagacgta
      481 gattcatggt cgccaagctc gagaacgagg acgacatcgc aagaatgcgc aacgcggggc
      541 ggcttgccgc cgaggtgctg gagatgatcg gcgagcacgt tcgccccggg gcctccaccg
      601 aggatctcaa cgggatctgt catcgctaca tcgtggagga gatcgactgt atcccggcgc
      661 cgctgaacta ctccaacggg cccggtcagc ccgcgtttcc gaaatccatc tgcacgtcgg
      721 tcaaccacgt cgtctgtcac gggattccgg cagcgaagaa ggtgctcaag agcggagata
      781 tcgtcaacat cgacgtcact gtcatcaagg acggcttcca cggcgacacc agcaagatgt
      841 tcatggtggg aaagccgagc gtcttggcag agcggctggt ccgcgtgacc caggagtgcc
      901 tgtaccgggg catcgacgcg gtacgcgcgg gcgcgacgct gggcgatatc ggtcacgcga
      961 tccagagcca tgctgaagct cagcacttct ccgtcgtgcg cgagttctgt ggccacggga
     1021 tcggccgggt ttttcacgac gaaccccagg tcgtgcacta tggccgcccg aacacgggaa
     1081 tgcgcctgga ggaaggcatg accttcacca tcgaacccat gatcaatgcg ggcagacgtc
     1141 acgtgaagat cctgcccgat cactggaccg tggtaaccaa ggaccacaag ctttctgcgc
     1201 agtgggaaca caccatctta gtcaccgcaa cgggtgccga ggtgctcacc cgtcgcagcg
     1261 acgaatcgtt gccttgatca cgaaggccac gtcgtcatga gcacgacaga tagagcggcc
     1321 cgatctccgg gcagcgacgg gaacgagggc agccgccgtt tcggcaacgc ggatcccttt
     1381 ctcgaagagc tgaaggttcg tcttgctcag gccgatgaac gtatggccca aagatactgg
     1441 gaccgtgccg acgtcgatga gctgatcgca gagcgcacga cgttcttcga cggcctgctg
     1501 cgcgaggtgt gggaccacgc cctgagcgaa gatgcgcaaa aggggctcgc cctcttcgcc
     1561 gtgggcggct acggccgcag agagcagttc ccctactcgg acatcgacct cctcatcgtc
     1621 ggcaagcggc cggccagcca ccgcgaggcc atcagccggt ttcttcaggt gctctacgac
     1681 ctgaacctgg aggtcgggca cagcgttcgc acggttcggg actgcaagaa agaggcgcag
     1741 aaggacatca ccgtggcaac ggccatcttc gagcggcgtt ttctgttcgg gtccgacgcg
     1801 ctgcggcgcc ggctcgatcg ggtgctgtcg tcatctcgcc tgtggccggc ggaggctttc
     1861 ttccgcgcca agcgcgacga gcaggcgcac cgccaccggc agtacgacaa cgtcgagtac
     1921 ggtctcgaac ccaacgtcaa atcgtcgccc ggcgggctga gggatctgca gacggcgctg
     1981 tgggtgtgcc agcgcaagtt cggcaccacc gatcctgaag ctctggaggc gcttggcatg
     2041 ctcacccccc tggaacgcga gtggctgacg gaaggccgcc gctatctgtg gtgggtgcgg
     2101 tttggccttc atctcatcgc cgggcgcaag gacgaccacc tgcagttcga gcaccagcgc
     2161 gctctcgcgc agcggctggg atttgccgac acgaacgccc gcctgggcgt agagcggttc
     2221 atgcacctgt actaccgtca cgtgctcgag cttcgcgagc tcaacgacat actgctccag
     2281 cacttcgagg aagcgatcct gagcagccgc ggccggccgc gcatcgagcc catcaacgag
     2341 cgttttcaga ttcacaacga ctacatcgaa gccaccgacc ccacggtgtt cgagcgatcg
     2401 ccccgagcca tcctggaaat cttcgtcatc atggccaatc ggcgggacat ctcaggggtg
     2461 cgcgcgcaaa ccatccgcct gatccgggac agccttcacc tcatcgacga ctcgttccgc
     2521 gccgacccgg agaacgcggc gctgttcatg gatctgctca aagcgccgta cacgctggtc
     2581 tcgcagctga cgaggatgcg ccggtacggg gtgctggcgc gctaccttcc ggagttcgga
     2641 cgcgtagtcg ggcaaatgca gcacgacctc ttccacatct acaccgtgga cgcgcacacg
     2701 atgatggtga tcggcaacat gcgccggttc cgctatcgcg cgagcgaaga gcgctttccg
     2761 gtcgcctgtc actgcgtgaa ggaaataccc aagccggaac tcctttacgt gtcgggcctc
     2821 taccacgaca tcggcaaagg gcggggcggc gaccactcgg agctcggcgc cggcgacgtc
     2881 accgagttct gtcgccgcca cgggctgaac gaagcagata ccgatcttgt ggcatggctg
     2941 gtcaggcagc acctgctcat gtcctccacg gctcagcgaa aggacatcta cgatccccag
     3001 gtcattcatg atttcgccac agaggtcaaa tcggagatgc gtctgaacta cctttacgcg
     3061 ctcaccgtgg ccgacatcaa cgcgaccaat cccgatcttt ggaacagctg gcgggcaacg
     3121 ctgctgcgcc agctctacgg ggagacgcgc aaagccctgc ggcggggcct ggagacgccg
     3181 ctggacaagg cgaccagcgt gaacgcctgc agggagagcg cccacgagcg cctcgtcgcg
     3241 gcaggcatga cgccggaagc catcgagcac gcctggcggg ttcccggcga cgagttcttc
     3301 ctgcgccaca gcccgcgcca ggtcgccgag gtcacccagc tcattcacgg acacgacgtc
     3361 cgcagcgagc ctctggttac cctgctcgac ctcaagggcc aggtttccca ggaaggcgcc
     3421 accgaggtcc tgctctacac gactgatcgg gagaacctgt tcgccgcgag cgtgctcgcg
     3481 ctgagccagc tggatctctc gatctacgac gcaaacattc acacttcagc ggacgggttg
     3541 tgtctgaaca cctacgtggt gctcactgct gagggcaagc ccctgcctcg tgagggagat
     3601 cggcgcgggc aggtcgtcga gaagctgacg cgatcgctgg cagacacctc gaccctgacc
     3661 ggcagcggca aacgtaggct ccctcgacag cttcgccagt tcaccttgcc gaccgaggtg
     3721 tctctgacga cgcccgcggg ctcttccaac tccgagctca ccatcatcgc gtccgaccga
     3781 cctggcctgc tcgcgaccat cgggctgctg ttcgcggagc tcgggctggt cgtcatcagc
     3841 gcccggatcg ccaccctggg cgaacgtgtg gaggacgtgt tcgagatcag cagccgggag
     3901 ggcgagccga tcacggacga ggaagccgtc tacctcctgg agaacaccct ccggcaacgc
     3961 ctggaccggc aggtcgcggc ttcctgaccc agggtgaacg cgaacctgga aaagctgcag
     4021 gcctacccct tcgagcggct ggctgacctt ctggccggcg tccggggcaa tgccgagttc
     4081 cctcacatcg cgctctccat cggcgagccg aagcacgccc cgccggagtt cgtgatcaat
     4141 ctgttgagcg accccgaccg gctgcgcgcc gggctgggcg cctatccagc cacgaggggt
     4201 attccggagc tgcgagaggc catcagcggc tggctggcga ggcgttacgg cgttcgcctc
     4261 gatcccgaca atcaggttct gccggttgcc ggcactcggg aagcgctgtt ctcattcggc
     4321 ctcgcggcgc taagcggcaa ggctgacgcc cgtgtgctga tgcctaaccc gttctaccag
     4381 atctacgaag gggcagcgct gctgggcaac ggcgtgccgc actacgtcaa ttgcgaccga
     4441 gcaaacgact accagccgga cttcgccggc gtgccggact ccgtctggag cgcttgcgag
     4501 gtcgtctatc tgtgctcgcc gggcaacccg accggagaga ccatcccgga ggacaccgtg
     4561 ctctggctgc tcgagcaggc cgaccggcac gacttcgtcg tcgccgccga cgagtgctac
     4621 tcggagatct atctggacga ctcacacccg cccgccggcc tgctggagat cgctcaccgc
     4681 gccggtcgcg acgactacgc ccgctgcgtt gctttccaca gcctttccaa gcgttccagc
     4741 ctgcccgggc tgcgttccgg attcgtcgcc ggcgacgccg caattctcgc cggctacttc
     4801 aaataccgca cgtacgaagg atgcgcgctg cccctgcacg tccagcaggc cagcgcggcc
     4861 gcgtggtcgg acgaagctca tgtggaagcc aaccgcgccc tctaccgacg aaagttcgag
     4921 acggtcggca gggtgctcga cccgcatctg gaccttcgca cgcccgccgg cggcttctac
     4981 cactggttgc acatcggcga ggacgacacg gcattcgccc gtcaactttt cgagcactgc
     5041 aacctgaccg ttctgcccgg ctcgtttctg ggacgggccg gcggcgactc gaaccccggc
     5101 ggtggtcacg tacgggttgc ctgggtcgcc tccgaagcac agtgcgtcga ggccgccgag
     5161 cgtcttgccg ggtggcttca gaaccgctag tgccggtacg tactaacggc cgagacggcg
     5221 ttgtggtagg ttacgcgcca cccgcatcgc gaggtcaata gcatgtcagg catattcgcg
     5281 ttcgcggtcg ggacggtgcg aagaaacaca gccggcgcgc cgctagactt ctacttcccg
     5341 gcccccgtca tcaacccgga accggacctg gcggcgcaga tcgaagggtc gttttccagc
     5401 ggcgctcatg aagtagggcc cgccgaactc gcccgcttcg ccaaggcagt cggcgactcg
     5461 gccatggccc ggctgctcag cagcctggct gagacagacc gggctatgac ggccgttcgg
     5521 ctcgacgctg acgagcccat cgactcgtcg gcgaccgcgt atctgaagct gcacctgctc
     5581 tcgcaccgca tggcccttcc gaacacgctg aatctggacg gcatattcgc tcatcttccc
     5641 aacgtcgctt ggaccgaccg cgggcccatc gatccggaag aactggcggc cgcacagctg
     5701 caggcaaggc tcgctggcgg gcacatcgag gttctggccg tggacaagtt cccgcgaatg
     5761 accaactaca tcgttcccag cggtgtgaga atcgccgacg ccagccgcat acgactcggt
     5821 gcctacctgg gcgacggcac caccgtcatg catgagggcc agatcaactt caacgctggc
     5881 gccctcggcc ccaacatggt cgagggccgg atctctcaag gcgtcatcct gggggagcat
     5941 tcagacctcg gcggcagcgc cagcaccatg ggcacgctgt caggaggcgg agagatcacc
     6001 atcagcatcg gcaagcactg tctgatcggc gccaacgccg gaaccggcat tccgctcggc
     6061 gatcgatgca cgatcgaggc cgggctctac atcacggccg gcacgccggt cactctcatc
     6121 gacgagacag gagaagatgt gcggacgatg aaggcgcgcg aactggcagg aggctccgat
     6181 ttgctgttca tccgcaacgg ccgatcggga cgcatcgagt gccgcagcaa ccgcaaagcc
     6241 atcgagctca acgaacagct acacgcacac aactgacaaa ccattgagcg aaggatcgca
     6301 aaatccggtt ctcgaactca ccgtggagct gatgcggcgg gccagcgtga ccccgagtga
     6361 cgagggctgc caggagctga tcggccagcg gctcgctgcg atgggcttcg tgaaccgccc
     6421 gatgaatttc ggtgatgtga gcaacctctg ggccacccat ggcgagggcg gcccgctatt
     6481 cgttttcgcc ggccacaccg acgtcgtccc ggccggaccg caggacgcat ggacgaaccc
     6541 gccgttcgag cccacgctgc acgacgggtt tctgtggggg cggggcgccg cggacatgaa
     6601 ggccagcctg gccgccatgg tcgtcgctac cgagcgcttc ctggcagctc acccccgcca
     6661 ccggggcacg ctcgcgtacc tgctgacttc cgacgaggaa ggaccggccg tggacggcac
     6721 ccggcgggtg gtggagacgc ttcaggcaga gcgcgcacag gagatggact tttgcgtggt
     6781 cggcgagccg tcctcggccc accttctggg cgacaccgta cgggtcggtc ggcgcggctc
     6841 ggtgaacgcg cgcctcacgg tattcggcac ccagggccac gtcgcgtacc cggagctggc
     6901 agagaatccc gtccacaccc tgctgcccgc tctggccgag ctgaccaaca aacgctggga
     6961 tgagggaaac gatggctttc cgccaaccgg tctgcaggtg tcgaacatcc acgcgggcac
     7021 gggcgcgccc aacgtcattc ccggatccct ggtggtcgac ttcaacatcc gcttcaacac
     7081 agagcagacc tcatccgggt tgcaccggca ggcggcggaa atactcgacc gtcacggcct
     7141 tcagtacgac ctcgaatggg tagtgtccgg cgagccgttc ctcacagcac ccggcgacct
     7201 gcgagccgcg gtcaataagg cggtgggcga cgtcaccggc agaacgccgg aggcatcgac
     7261 ctctggcggc acgtcagacg gtcgcttcat tgcccccacg ggctgcgagg tcgttgagct
     7321 gggccctgtg aacgcgacga ttcacaagat cgacgagcgg gtggcgaccg ccgacctgct
     7381 cccactggca gacatctacc agcgcgttct cgatcagttg ctcggctgaa catgcgcacc
     7441 cttattctgt taggttttct gctctttctg acgttctgca tcgtctttgc gccggccggc
     7501 ctcaccggcc tcgtcctgga ccgggtgaac ggcgtcgacc tgacgcgggt gaacggaacc
     7561 ctttggcagg gtggaggcga tctgtacgtt cgctccgagc ccgtgggcag gcttcagtgg
     7621 gacgtccatc cggcaaccgt tgtggcgggc gcgctgggct gcgactttac ccttagccat
     7681 gcgcaccacc agttgcgggg taccgccgtt gtgggattga atcgaacgtt taccctggac
     7741 gtatcgggta aagtcggcgc ccaaacggta aacgggtggc tcgagccgta cgacatgacc
     7801 atctccggca ccatggatct gtcaaacgtc ctgctgacat tcgacgacgt tgcgctgcgg
     7861 gacgcaaatg gagtggtgga atgggaaggg ggcagcatca gctatcccgc cggcaatggc
     7921 attcagtcaa gcatcctgcc gcccctgcgg gcgaccctgg gccccggacc cgccgctacc
     7981 atcacgacgc ttgacgaccc ggcggctgcg ctggtgaccg ccgagctgct cgacaacggt
     8041 attgccaagg taggcgttac cgcacgcatg accaggctcc tgaatctctc gtggaccgag
     8101 acggagcccg acagcgcggt agtgctagag gtggaagaac aagtttttta gtatcgacac
     8161 acatggccgc cctactcaac ttcatcgtga cacgcctggc cgaacccgct cgctggctgg
     8221 tcgtcgcgct gatcgcctac acgctggcaa ccacggtgct ctacctggta tccgcgcccc
     8281 aatcggatgt tggtgcgggg tcggacgcca aagctccaca ggggcagccg gggcgcgccg
     8341 gcaccggcca gcagggaccg gttgacatca atcggatcgt ggcgcgcaac ctgttcggcg
     8401 ttgccgacgc atcggcgcct gccgccacgc agcagacggc catggccgtg gccacgaggc
     8461 tgccgctgga gttgcagggc gtcttcgtgg cagacgcatc ggaagactct gccgcgatcg
     8521 tcgcccagaa ggggcgcccg ggaatgctct acgttgtcgg ccagaaaatg cccggtaacg
     8581 cagagctcgt cgaggtgcac gccgatcaca tcgtgcttcg gcgggccggc gtcctggaga
     8641 cgctgaagtt tccgacatcg atcggccagt tcgtagccgc ccccgatgat ctggaggatc
     8701 ttgccgacca ggcgctcccg ttcgacatgc cgaccgacct ccaggaggat gccgacgacg
     8761 atcgggatga gcggggccgc aagagaccca ccagcgaagc gctggccgag taccgggagc
     8821 gtatcgaggc cgatccccag ggcgtcctcg ccgacctggg tatctccgcc gtgaacgaag
     8881 gcgacgcctc cggctaccgc ctggacgatc tgaacgactc accatttctc agccagacgg
     8941 ggctgcagcc cggggacgtg attctttccg tcaacggtcg acccgtcggg gacatcaacc
     9001 aagaccagca ggaactggac aacatgctgg cccagggatc cgctcgcctc gaagtacagc
     9061 gcggaacgcg tcggttcttc gtgaccgcct ccttgccata ggacccgttg aaaaacgcat
     9121 gaataagata ttcgccacca ccctaaccta cgcgatggtc cttcttctgc tgctgcccac
     9181 gagcttgccc gcgcagggac aggagcagac ctggaagatc aacatcaaga acgccgacat
     9241 ccaggagttc gtcgcccagg ttgccgccat taccggcaag acgttcgtca tcgatccccg
     9301 gctgaaaggc aacgttaccg tcatttccaa caccagcatg gacgaggaag ccgtctacgc
     9361 gctgttcctc tccgtcctgc gcgttcacaa cttcacggca acgccatcgg gtgacgtgat
     9421 ccgtatccag cagaacgcta ccgggaagca gacgccgggc gtgagcgggg agctcggcga
     9481 agtgccgccc gaagagctcg tgacccgggt tgtgtcggcc cagaacgtgg agtccgccga
     9541 gctcgtcaag atcctccggc cgctcatccc ccagtacggc cacatcgccg ccgttgccgt
     9601 gcccaacgtc gtcatcatca gcgaccacgc cgacaacatc ctgcgcctga tgaggatgat
     9661 cgagcagatc gacgtagccg acgaagagga aatcgtggtg gtaccgctca aggaagcctg
     9721 ggtcggaacg atcgtcgcgc tgctggaaaa ggtggcgcca gatcagatgg gccgcaacgc
     9781 tcagggcccc cagcgcattc agatcatcgc caacgagcgc aacaactcgc tggtgctcaa
     9841 aggcaagccg cggcccgttg ctgaggtgct gaagatcatc gagaagctcg accagccggc
     9901 aaccaccgcc ggcgcgactc aggtcatcta cctcaagcac gccgacgcgg taaaggtttc
     9961 cgagatactc aactcggtca tcgaggaccg ttcccgtggc gaagacggcg ccacggagcg
    10021 cccgaacatt caggccgacg agacgctgaa tgcgatcgtg gttcgagccg atccaggcac
    10081 catgaacgag atcctcgaaa ttctggaaag cctggatgtg cggcgaaccc aggttctcat
    10141 cgaggccgcc gtggtggagg taaccatcac gaacgcctcg gatatcggcg tggagatggc
    10201 catcgtggac gagtccggcc agaccgtacc cctggccagc acgagcctcg acggcgcgat
    10261 caacagcctc atcggcaaca tcatcggcga cgacggagag atcgatccgg tcagcgggct
    10321 cgcctccatc acgagcccga ccctggccgc tgcgcgcatc gacaggggag acgtctcctt
    10381 cggcgccatc gttcgcgcgc tcgccaccaa ctccaacgcg aacctgctgt cgacgcccag
    10441 catcctgacc ctggacaacc aggaagccaa gatcgtcgtc ggtaacgagg ttccgttccg
    10501 gaccggatcg ttcaccacca gcggaaacga cagcagcaac ccctttacca ccgtccagcg
    10561 tgaagacgtg ggcttgcagc tcaccgtgac gccccacgtg cacgagggca cttcggtaag
    10621 gctcgaggtc gcccaggaaa tcaccaacgt gatcaacgcg cccatcggca acgacgggtt
    10681 ctcggacgtt gtcacgtcga agcggagcat cgagaccacg atactggcgg aagacaggca
    10741 gaccgtggtg ctcggcgggc tgattcagga cgacatcacg gacacggtga agaaagtgcc
    10801 gctgctcggc gacatccccg gtctgggcgc gttgttccga tcgaacagca agtcacgttc
    10861 caagagcaat cttctggtgt tcctccggcc cacggtgatc cgcaacaagg aggactcgga
    10921 taccgccacg aacaggaagt acacggacat ctgggaggtg gaaatcagct cgggcggcgg
    10981 gaaatccgac atcacggata acttcagggg gcgcaaggat tagcatgcgt gcccccaaca
    11041 cctaaaggcg gtccatgcga caactcagac ggctcggcta cctggtcagc cgctggctca
    11101 tccggtcgat catacgtccc cgcctcatcg gcgaccctcc cgatccgccg cgggaaggcc
    11161 gacccgtcta cctgtactcg ctggaaaacc ggtcgctgag cgatctgatc gtcctcgacc
    11221 tcctcaacga ggccaaccac ctgccaggcc ccctggaccc ggtgactctg ggaagccaca
    11281 ccgaaggccg ccgtgtgttc tttctcaacc gggcgagcta cggcctattc caacgcaacg
    11341 tcatgagcac gccgtcggaa cggctgatcc gcgtgctcac ccacgcgggc gaagcgaacg
    11401 acgcggtaat cgtgcccgtt accgtcctct ggggccgcgc gccgtcgcgt cagggctctt
    11461 tgttccgctc actgctggcc gaggactggg cggtaaccac gcgcctgaag cgcatcctga
    11521 acctcgtcgt cagccgcaag gaaatatacg ttcactacgg cacccccatc tcgatcgggg
    11581 aactcgccga agacgacctc gatgtcaatc ggatggtccg ccgggccgcg cgcctgcttc
    11641 gagtgcagtt ccgtaaccag cgcgtttccg ccatcgggcc cgacttttcc catcagcgca
    11701 cgctcgtcaa ccagatactg cgcagccgcg cagtaaccga cgccatagcg gcggcagacc
    11761 ccgaagagaa gccggagcgc atggaacgcc gagcacgcaa gtacgcgatg agcatcgtct
    11821 cgaacatgtc gtatcccacc atcagggtac tcgagcgtct gctcacctgg ttc
//
LOCUS       DBNHLM010000240         6151 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1232269, whole genome shotgun sequence.
ACCESSION   DBNHLM010000240 DBNHLM010000000
VERSION     DBNHLM010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1232269"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..254
                     /locus_tag="AC16QF_11040"
     CDS             81..254
                     /locus_tag="AC16QF_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449135.1"
                     /translation="MVGLQSYVGELVMYRGFFYEIVRIAVRGDEVRCVAYSDELNREA
                     ELSIADVFLGLAA"
     gene            384..1934
                     /locus_tag="AC16QF_11045"
     CDS             384..1934
                     /locus_tag="AC16QF_11045"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6449136.1"
                     /translation="MTETLTGTNRSAGITYSELLDQDSHPVRDVLRLDSPMEPGPTKV
                     PVERYFSKAFHDLEVEKVWKRVWQMACHQDDIPEVGDYHVYDIAHLSFLIVRTGADEF
                     KAFWNACLHRGRQLKENHGTRARAFRCPFHGWAWNIDGTVLDIPCQWDFPTLDLDELR
                     LPEVQLGRWGGFIFINPDDDNAQSLEDYLGNLSDHFTLLPYERRYKSAHVAKVLRCNW
                     KVAQEAFSEAYHVIATHPTILETIGDANSKYDIFGNYSRAISPAFTPSPHLANMPHYD
                     PLPDARLYKKLRHPLSGYIYERGEDGLVHVIDHQGRISKFTAAGEWVEGEIRNADPNM
                     TDWIGGKQLPDSDDTSGLLPDSLPPDGNLRAMMAQGPRESLRPLLGDRVDEFSDAELL
                     DSIYFTVFPNFHPWGSFNQIVYRFRPHGDNPDECIHECMYFSPVPEGEERPPAAPIHW
                     LGADDDWVDAAELGMLAKVFNQDVVNMPYVQKGLKTMKQPYVIFADYGETKPRHFHKL
                     LNDWLDKP"
     gene            2111..3649
                     /locus_tag="AC16QF_11050"
     CDS             2111..3649
                     /locus_tag="AC16QF_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013729.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HIR6449137.1"
                     /translation="MADADQGFAYEFGDWLVEPDLNRIRRGEQEIQLEPSTLEVLCYL
                     LERPGEIVSVDELLDSVWRDSAAEPSAVHSNISRIRRALGESRREPRYIETVSTDGYR
                     AIGAVRRIEAGPHDAELAEALEAVTPPYPAYNGDEPYTFVCYSHQNREQVYQELVRLR
                     SAGINVWYDEGISPGAEWTEEVANAIANCTHLLFFVSTDSVSSKHCLDELSYAQERHK
                     SIVVVYLEPTELPQGLQLSIGRIQALFKYAMRESEYARKLIATLHGAAETRGASTARP
                     TAPRSPAPPSAGVPGRALRRSLSALGIAALIGALGLLVLRGPRIETILRDEPLENTIT
                     VEAFKDLTAAHDQWIGDTIESEVRVALPGRGLQVVGSASYQGARETSVRERARYRITG
                     TVKRLRSRIRVTAELIANDTDYMIWSEIYDEQITQDAAAQSEIAVRIVADIARKIQGG
                     AEQPLIATIPSARPAAGALQGALDAQPSVPEFSDFDAQEDGDPGQPAFDAFPSFDEDG
                     GGDD"
     gene            3642..5801
                     /locus_tag="AC16QF_11055"
     CDS             3642..5801
                     /locus_tag="AC16QF_11055"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6449138.1"
                     /translation="MISRLHRLREVALLVVTVLVVPIAHGDDIGELLRPADVRSVIIS
                     PDGRHLAMVRSDDQKDTLVIFARPAMTVATGVASSPGQRFETITWANNRLVLIEPAAD
                     AGAAQQPVPTGAMLALGVDGSRKRLLPRNPEAVRLPTAVISVLPNDLANVLIAAHSAC
                     DAESCSEAEKALRRPVKLNLETGEKTFVAPALAMPAYYVSDPSGNRMLAAGRAADGRV
                     EVYRLAAGSWTPVSSFDPAADLGTVPLAVDRAGSVFALANKVGTAGLYRWNLDSGETD
                     EIYRGAGSDIDRVITSYGGRRLLAVRSDPGYASWHYLDPEHPFTRIHTALRAAFPDSD
                     VDVTSFTAEGSEAVVRIYSDRNSGDFYSVSLGTGESTLLATSRPWLPAEDLAKVEPME
                     LTSRDMFTLRGYVTTPASAGPHPMIVWIHDKPTTTRAVWELNAEVQMLARRGFAVLQI
                     NYRGSGGLGQWYTASGAERNGQAVQRDIADATRWAIDNGIANEGQICVYGRGYGAFAA
                     VKAMATNIGLFQCAVAIDGFYDLASPFTVPEDPTLTPQNLLTFPSMLDLDDVRRSPVN
                     RVFNIDGSVLLVGESEQTITMRDALEETEKSVVWLAPENEQSGYSEILAYLEAELSAG
                     KAADTDPAGFGATLTARQAREFQRIREEMRAATKELGNRRAFTAAGVRREVEKIIEGY
                     DSDVRSIVTDDQWALYDDFKVGLAQELESELDIVRLQ"
     gene            complement(5875..>6151)
                     /locus_tag="AC16QF_11060"
     CDS             complement(5875..>6151)
                     /locus_tag="AC16QF_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449139.1"
                     /translation="ECIMECMYLAPIPDDGDYTPVADIHWLGPDDDWTDAPELGSLAG
                     VFNQDVRNMAFVYDGMKASARECVRLADYNETKIRHFHALYGEWVDG"
BASE COUNT         1173 a         2045 c         1891 g         1042 t
ORIGIN      
        1 tgacataccg cttacgctta ctcacaccac aaaccgagaa acggatcaca atattcgggt
       61 cattttctgg ataaccaccg atggtcggat tgcagagcta cgtaggcgag ctggtcatgt
      121 accgcggctt tttctacgag atcgtgcgca tcgccgtccg cggtgacgag gtccgctgcg
      181 ttgcgtacag tgacgagctc aacagggaag ccgaactctc catagccgac gtgttcctcg
      241 gtctcgcggc ctaacccacc tgcgcctcct cctctcgacc ggcggcacca ggcctttatg
      301 ccggacccct cgggtatccc gtattgcggg ttctacgggg cgataccagc atggcccgct
      361 gcgcaagagt cggggatcca tccatgaccg aaacactgac tggcaccaac cgctccgcgg
      421 gcatcaccta cagcgagctg ctcgaccagg acagccatcc ggtgcgcgac gtcctgcggc
      481 tcgattcgcc catggagccg ggcccaacga aggtaccggt ggagcgatat ttctccaaag
      541 ccttccacga cctggaggtc gaaaaagtct ggaagcgggt ctggcagatg gcctgtcatc
      601 aggacgacat tccggaggtc ggcgactacc acgtatacga catcgcccat ctctcatttc
      661 tcatcgtccg caccggtgcc gacgagttca aagcgttctg gaacgcctgc cttcaccgcg
      721 gccggcagct caaggagaat cacggcacac gagcgcgcgc gtttcgatgt ccgttccacg
      781 gctgggcctg gaacatcgac ggcacggtac tggacatccc ctgccagtgg gattttccga
      841 ctctcgatct ggatgagctg agactgcccg aggtgcagct gggccgctgg ggcggtttca
      901 tcttcatcaa cccggacgat gacaacgccc agtctctgga agactaccta ggcaacctct
      961 ccgatcactt cacgctgctc ccctacgagc ggcggtacaa gtctgcccac gtagcaaagg
     1021 tgctgcgttg caactggaag gtggctcagg aggcgttcag cgaggcctat cacgtcatcg
     1081 ccacccaccc gacgattctc gagaccatcg gcgacgcgaa ctcgaagtac gacatcttcg
     1141 gcaactactc acgggcgatc tctcccgcct tcacgccgag cccgcatctg gcgaacatgc
     1201 cgcactacga tccgctgccc gacgctcgtc tctacaagaa gctgcgtcat ccgctttcgg
     1261 gctacatcta cgagcgtggc gaggacgggc tcgtccacgt catcgatcac cagggccgca
     1321 tcagcaaatt caccgccgcc ggcgagtggg tcgaaggaga gatccggaac gcggacccga
     1381 acatgacaga ctggatcgga ggcaaacagc tcccggacag cgacgacacg agcgggctgc
     1441 tgcctgactc ccttccaccg gacggcaacc tgcgcgccat gatggcccag gggccgcgcg
     1501 aatcactcag gccgctactg ggagatcggg tcgacgagtt cagcgacgcc gagcttctcg
     1561 actccattta cttcactgtg tttcccaact tccatccatg gggatcgttc aaccagatcg
     1621 tttaccggtt ccggccccac ggggacaacc ccgacgagtg catccacgag tgcatgtact
     1681 tctctccggt gcccgaaggc gaggagcgtc cgccagctgc gcccattcac tggctggggg
     1741 ccgacgacga ctgggtggat gccgccgagc tcggcatgct ggccaaggtg ttcaaccagg
     1801 acgtggtcaa catgccctac gttcagaagg gcctgaagac catgaagcag ccctacgtga
     1861 tcttcgccga ctacggggaa accaaacccc gtcactttca caagctgctc aacgattggc
     1921 tcgataagcc ctgatctgat cggcaagggc tcgggtgcct aagccggcga cccatcgccg
     1981 gcgctatcgc gatccggtac gcgtgtggcg agactcaccg gcccgagagc cacggcgccg
     2041 ccgcgcggca gtcatcatga tggagtaagc taggcccgac gacgttgggc ggcaggatgc
     2101 gtgcgggtct atggccgacg ctgatcaggg cttcgcctac gagttcggcg actggctggt
     2161 cgagccggat ctcaaccgaa ttcgacgggg ggagcaggag atccaactcg agccgagcac
     2221 gctcgaggtg ctctgctatc tgctggaacg tccgggcgag atcgtgtcgg tcgacgagtt
     2281 gctggattcg gtctggcgcg acagcgccgc cgaaccgagc gccgtccaca gcaacatcag
     2341 ccgcattcgc cgtgccctcg gtgaaagcag acgcgagccg aggtacatcg aaaccgtctc
     2401 cacggacggc taccgggcca tcggcgcagt tcgcaggatc gaagcgggcc cccatgacgc
     2461 cgagctcgcc gaggccctgg aggccgtcac tccgccctac cctgcgtaca acggtgacga
     2521 gccctacacc ttcgtctgct actcgcacca gaatcgcgag caggtgtatc aggaactcgt
     2581 gcgccttcgg tcggccggca tcaacgtctg gtacgacgag ggcatctccc cgggggcgga
     2641 gtggaccgag gaggtggcca acgccatcgc caactgcacg cacctgctgt tcttcgtctc
     2701 aacagactcc gtttcatcca agcactgtct ggatgagctt tcgtatgccc aggagcgcca
     2761 taagtcgatc gtggtcgtct acctggaacc cacggagctt cctcagggcc tgcagctctc
     2821 cattggcaga atccaggcgt tgttcaaata cgccatgcgg gagtcggagt acgcccgcaa
     2881 gctgatcgcc accctgcacg gggcagccga gacacgcggt gcaagcaccg cacgacctac
     2941 cgcaccacga tccccggcgc cgccatcggc aggagtcccg gggagggcgc tgcgccgctc
     3001 gttgagcgcg ttgggcatcg ccgcactgat cggcgcgctt gggttgttgg tcctgcgtgg
     3061 cccgcgcatt gagacgattc ttcgcgacga gcccctggag aacaccatca cggtggaggc
     3121 cttcaaggac ctcaccgccg ctcacgatca gtggatcgga gataccatcg agtcggaggt
     3181 ccgggtcgcg cttccaggca gaggcctgca ggtagtgggc tctgcatcct accagggtgc
     3241 gcgcgagaca tcggtgcggg aacgggcccg ctatcggata accggcacgg tcaagcgatt
     3301 gcgcagccgc atccgggtga ccgccgagct catcgccaac gacacggatt atatgatctg
     3361 gtcggagatc tacgatgagc agatcactca ggacgccgcg gcccagagcg agatcgccgt
     3421 aaggatcgtc gccgacatag cccgcaaaat tcagggcggt gccgagcagc cgctgatcgc
     3481 aaccataccg tccgcaagac cggcagccgg cgccctgcag ggcgcgctcg atgcccagcc
     3541 ttccgtaccc gagttctccg atttcgacgc gcaagaggat ggcgacccgg gccaaccggc
     3601 cttcgatgca ttcccgagct tcgacgaaga cggaggtggc gatgattagc cgtctccatc
     3661 gactgcgcga ggttgcgcta ctcgtcgtca cggtcctggt cgtcccgatc gcccacgggg
     3721 acgacatcgg cgagctgttg aggccggccg acgtgcgctc ggtgatcatc tcaccggacg
     3781 gtcggcacct ggcgatggtc cgaagcgacg atcagaagga cactctggtg atattcgccc
     3841 gtccggccat gaccgtggcc accggcgtcg catcgagtcc tggtcaacgc ttcgagacca
     3901 tcacctgggc gaacaaccgc ctcgtgctca tcgagcctgc agcagatgcc ggcgcggctc
     3961 agcaaccggt tcccacgggc gccatgctcg cgctgggcgt ggacggctcg cgcaagcgcc
     4021 tgcttccccg taacccggag gccgtcaggc ttcccaccgc ggtgatcagc gtgctgccca
     4081 acgacctcgc caacgtgctc atcgcggccc acagcgcctg cgacgcggag agttgctccg
     4141 aggcggagaa agctcttcgc aggccggtca aactcaatct cgaaacgggc gaaaagacgt
     4201 ttgtcgcccc tgcccttgcc atgcccgcct actacgtcag cgacccgagc ggcaacagga
     4261 tgctggctgc cggccgcgcg gcggatgggc gggtcgaggt ctatcggctg gccgcaggca
     4321 gctggacgcc cgtgtcatca ttcgatcccg ccgccgatct cgggacggtg ccgctcgccg
     4381 tcgatcgggc cggcagcgtg ttcgccctgg ccaacaaggt cggcaccgcc gggctctaca
     4441 gatggaatct cgattccggg gagaccgacg agatctatcg cggagccggc tccgacatcg
     4501 accgggtcat cacgagctac ggtggccgca ggctcctggc cgtgcgttcc gacccgggct
     4561 acgcaagctg gcactacctg gatccggaac atcccttcac caggattcac accgccctgc
     4621 gcgcggcgtt cccggacagc gacgtcgacg tcaccagctt caccgcagaa ggttccgagg
     4681 ccgtggtccg aatctacagc gaccgtaatt caggcgactt ctacagcgtg agccttggga
     4741 ccggagagag cacgctgctt gccacgagcc gaccctggct gccggcggaa gaccttgcca
     4801 aggtcgagcc catggagctg accagccggg acatgttcac tctgcgcggc tacgtcacca
     4861 ctccggcatc ggccgggcct cacccgatga tcgtgtggat ccatgacaag cccaccacca
     4921 cccgggcggt ctgggagctc aacgccgagg tccagatgct ggcgcggcgc ggtttcgccg
     4981 tcctgcagat caactaccgg ggatcgggcg gtctgggtca gtggtacacg gcctcgggcg
     5041 cagagcgaaa cgggcaggcc gttcagcgtg acatcgccga tgccacccgc tgggccatcg
     5101 acaacggcat cgccaacgaa ggccagatct gtgtttacgg gcgaggctac ggggcctttg
     5161 ccgcagtcaa ggccatggcc accaacatcg gcctgttcca gtgcgccgtc gccatcgacg
     5221 ggttctatga tctggcatcg cccttcaccg tcccggaaga ccccacgctg acgccccaga
     5281 acctgctgac attcccctcg atgctcgatc tggacgatgt caggcgctct ccggtcaatc
     5341 gcgttttcaa catcgacggc tcggttctgc tcgtcggcga atccgaacag acgatcacca
     5401 tgcgcgatgc gctggaagag accgaaaagt ccgtggtctg gctggcaccc gagaacgagc
     5461 agagcgggta ctcggagata ctcgcatatc tcgaagccga gctgagcgcg ggcaaggcgg
     5521 cggacacgga tccggccggc ttcggcgcca cgctgaccgc acggcaggcc agggagttcc
     5581 agcggatccg ggaagagatg cgagccgcga ccaaggagct gggaaatcgc cgcgcgttca
     5641 ccgccgccgg cgtgcgccgg gaggtggaga agatcatcga aggctacgac tcagacgtcc
     5701 gatccatcgt caccgatgat cagtgggccc tctacgacga cttcaaagtc ggtctcgccc
     5761 aggaactgga aagcgagctg gacatcgtca ggctgcagtg agtcggagct cgcggcccgt
     5821 tcgcgccccc attcagcggg ccgacccgga agccggcatg accggaaagg atcgtcagcc
     5881 gtccacccat tccccgtaaa gcgcgtgaaa gtggcggatc ttcgtctcgt tgtaatcggc
     5941 cagccgcacg cattcccggg ccgatgcctt catgccgtcg taaacgaagg ccatgttgcg
     6001 tacgtcttga ttgaaaacac cggccaggct gccgagctcg ggcgcatccg tccagtcgtc
     6061 gtcgggaccg agccagtgaa tgtccgcaac cggcgtgtag tcaccgtcat cgggtatggg
     6121 cgcgagatac atgcactcca tgatgcactc t
//
LOCUS       DBNHLM010000241         9822 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1170881, whole genome shotgun sequence.
ACCESSION   DBNHLM010000241 DBNHLM010000000
VERSION     DBNHLM010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9822)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9822)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9822
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1170881"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="AC16QF_11065"
     CDS             <1..203
                     /locus_tag="AC16QF_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007999939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR6449140.1"
                     /translation="IEIRGFGSFSLHYRAPRIGRNPKTGESVGLAGKYVPHFKPGKEL
                     RDRVNQGLERERAEQESAGLPS"
     gene            213..506
                     /locus_tag="AC16QF_11070"
     CDS             213..506
                     /locus_tag="AC16QF_11070"
                     /inference="COORDINATES: protein motif:HMM:NF018058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HIR6449141.1"
                     /translation="MAWLSRFFWALLALLVFCLAGLAVNQEPISLKFVTWQTPQLSVF
                     WWLLAAFSTGVLLGLVGISVVRARLGLKNRSLVKRLEASERELRRLRSVSYHE"
     gene            513..1685
                     /gene="lapB"
                     /locus_tag="AC16QF_11075"
     CDS             513..1685
                     /gene="lapB"
                     /locus_tag="AC16QF_11075"
                     /inference="COORDINATES: protein motif:HMM:NF008757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HIR6449142.1"
                     /translation="MPENLVVYLLLVLALGIGFLLGRRERKRKQSSPGMVAQEYFRGL
                     NHLLNERHDLAIDTFVEAMAVDSDTVDTHLALGSLVRRRGEVDRAIRIHQNLLARPVL
                     SPAHRAQTELELARDYLSAGLLDRAENLLLELVKKPGDDRDVARTMLLEIYQREREWE
                     RAVEVARELSRADRSYRTPLAHFQCELAEKALADGDLRRARADLERAGDFDPGCARVA
                     LVGAKVASAGGRHRDVKRLLRKARSLEPELSGETVAMFRAASEELGDRADFVRYLREC
                     LERAAPLEVVQALTREIEADEGDEVAREFLIEQLLRNPNLAGFVSLLEQLDRGGAALE
                     ADQLALVRRFSSSLLQKQPSHRCKDCGFSGHMLIWQCPSCKTWGSIKPIVRYDGDV"
     gene            1811..2092
                     /locus_tag="AC16QF_11080"
     CDS             1811..2092
                     /locus_tag="AC16QF_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HIR6449143.1"
                     /translation="MTMTIKRAMGAALLLAGVLAGPVLAAPESVNINSANAETLAEVL
                     NGVGLTKAQAIVEYREQHGAFRDAYELANIKGIGERTVEKNEARIRLQD"
     gene            2392..3513
                     /locus_tag="AC16QF_11085"
     CDS             2392..3513
                     /locus_tag="AC16QF_11085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6449144.1"
                     /translation="MKIIYVVNEGRFFLSHRLALAVEAKRHEVEVVVVCGANTGEQGL
                     ADAGLRYVTVPMSRSGFNPFKELRTAAALLRIYRAEKPDLVHHVTIKPVLYGTSMARW
                     SGVPAVVNAVPGMGFVFTRRGPLARIRRSVVQLLYRLCMFHPNMRVIFQNNEDMQAFT
                     QRGIVDRQHVALIRGSGVDLDVFRCAPEPPGPVTFVLVGRMLRHKGVYEYVEAARRVR
                     QRRPDWRFQLVGDVDPGNPASLRPDELIAWQQAGEIEWLGQRRDVPEVLAAAHVVCLP
                     SYREGLPKTLLEAAACGRAMIASDIAGCREVVRPNVTGLLVPPRVSEPLADAMLRLGE
                     DDALRHRFARAARSAAEEVFSLEDVVRHTFRVYDELLLP"
     gene            3510..4439
                     /locus_tag="AC16QF_11090"
     CDS             3510..4439
                     /locus_tag="AC16QF_11090"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6449145.1"
                     /translation="MRRAVVTGAGGFIGTHLCRALRHEGWEVAVITRAHALPGCERLP
                     VTLASADVELSRALGGAQVVFHLAGLAHGRASGAAEDELHRVNVESSARLLAASEAAG
                     VPAMIWLSSIKVLGDTSERPLRPEDAYRAGDAYARSKVEGERALLDREGFTRAAIVRP
                     PLVYGPGVAGNFASLARWVGSGVPLPLGAASAPRSMVGVDNLCSLMLRLAEEGSGVFH
                     VADARDRSVAQLIRAMAVLLGVPDRSWRVSPRLLAAVASALGQRARYTRLFEPLQVDQ
                     SMTRERLGWQPPHDPDQQLKETMRWFRERRQAT"
     gene            4409..5392
                     /locus_tag="AC16QF_11095"
     CDS             4409..5392
                     /locus_tag="AC16QF_11095"
                     /inference="COORDINATES: protein motif:HMM:NF013146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIR6449146.1"
                     /translation="MVSGTATGYLIAGFAASTVLVFLMLRVGRALNLMAVPNARSSHV
                     VPTPTAGGLGFVIPLLVFFGMLYLDGVDVALGLLLSLVLVAAASLWDDFAELSAGLRF
                     TCQTAAVALIVWQLDLPWAWYLLGLTGLALLWHVNLFNFMDGIDGIAAGQCLLYVVGV
                     QVLTLGAPGWMGELCWLTAGAMLGFLAYNWPPAKIFMGDVGSAVLGLLLALLALLLAR
                     DGVLPLIASLILLAGFWFDATYTLCVRMATGQAFTQAHRSHLYQKLAAFKGHLWTTGV
                     FIAFGLFWLLPLAWLTLRVADLAWVWLVAAVVPIGVASMRFGAGLPAQDEG"
     gene            5421..7331
                     /locus_tag="AC16QF_11100"
     CDS             5421..7331
                     /locus_tag="AC16QF_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012271055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR6449147.1"
                     /translation="MSRRKRQAIAVVTDFAVLPIALWTAIALRLGQVNPEVAQFWPAF
                     VVSSLLCIPVFGALGLYRHVVRHTGSEAIWAIVKGATIIALVVAAVAYMIPLPGFPRS
                     VPIIFWLLTLLYVSGTRFSMRAYFQWLQNRVRLRSPAVIYGAGSKGVELARVLSQQGD
                     YLPIAFIDDDRALHKRVIDGLNVYPPKSLGRVLRDSGARDVMVAIGSAEPDERRRIIE
                     FLEPFPARVRLIPDLVELESRRQTIANIRDVEIEDLIGRNTVSPLPHLLRGSVEGRSV
                     LVTGAGGSIGSELCRQIVRQGPQRMVLLDQSEFGLFEIQRELTQLIVSEGLPVDVVAV
                     LGSVTNGTLMRRNMESYAVETVYHAAAYKHVDLVEHNIIQGLRNNTFGTLYTAQAAMD
                     AGVREFILISTDKAVRTTSVMGASKRLAEMVLQALQEISDSTRFSMVRFGNVLGSSGS
                     VVPLFAEQIAQGGPVTVTHPEVTRYFMTIPEAAELVLQAASMAEGGDVFLLDMGEPVK
                     IRELAERMIRLKGLTVRTSSQSGGDVEIRITGLKPGEKLHEELLVGDEVIGTEHAKIM
                     RAEETFLPWPALRGALNTLEQACDAYDYDAIKQFIEGLVEGASLESQLGHLNAGPRVL
                     QLDPNRESPSQP"
     gene            complement(7353..8837)
                     /gene="tcuA"
                     /locus_tag="AC16QF_11105"
     CDS             complement(7353..8837)
                     /gene="tcuA"
                     /locus_tag="AC16QF_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019690203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HIR6449148.1"
                     /translation="MARTPTVIVVGGGNAALCAAISAAEHGADVRLLERAPETLAGGN
                     SRFTAGAMRVTYDGVEDLKRLMPELTDAEIARTEFGSYPRDAFFDDMGRITHYRTDPE
                     LAETLIDSSFSTLVWMRDQGVRFLTLYGRQSFEVDGKARFWGGLTVEAWGGGPGLVEK
                     LTEVARGRGVDIAYSARAVALDHDGQRVVGVRAQTPAGLETIAGDAVILASGGFESNA
                     AWRARHLGPGWDLARVRGTRFNTGDGIRMALEVGAAARGHWSGCHAVAWDLNAPEYGD
                     LSVGDGFQKHSYPLGILVNNAGRRFVDEGADFRNYTYARYGAEILAQPQQTAWQVFDA
                     KVAGLLRDEYRIRAVTRVVADSLQSLAEKMEGMNVSGFRSTVEAFNRAVRTDVPFNPS
                     VKDGRGTVGLAIPKSNWANQLDTAPFEAYGVTCGITFTFGGLHTDSRARVIDQGGEPI
                     EGLFAAGELVGGLFYFNYPGGTGLTSGSVFGRIAGREAAGGRSA"
     gene            8927..>9822
                     /locus_tag="AC16QF_11110"
     CDS             8927..>9822
                     /locus_tag="AC16QF_11110"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR6449149.1"
                     /translation="MTLPRMSFAMVQTDVRKLEPMELPLPEIDEDSALLKLEACGICG
                     SDHEQYEGLLRTPMPVVPGHEPLGTIAKIGDRAATRWGVDQGDRVAVETMISCRHCRP
                     CLSGSYHLCDHRLIYSYVPVSEAPGLWGGYSQYMYLHPNSIVHRVSPELPAEIGVMFN
                     PLGAGFRWAVELPATRPGDTVVILGPGQRGLASVIACREVGAGTIIVTGLSADEKKLQ
                     LAREFGADYTIDVDSEDTVKRVKAITDGRGADVVVDVSAYATKPVSDALSLVRPGGTV
                     VLAGVKGFKAVEDFVSDLVVLK"
BASE COUNT         1524 a         3105 c         3364 g         1829 t
ORIGIN      
        1 ggatcgagat cagaggcttc ggcagctttt cgctgcacta ccgggcgccg cgaatcggcc
       61 gtaatccaaa gaccggcgag tccgtggggc tggcgggcaa gtacgttccc cacttcaagc
      121 cgggcaagga gctgcgagat cgggtgaacc agggtctgga gcgggaacga gcggagcagg
      181 agtccgccgg gcttccgagc tagcctaagc ccatggcttg gttgtcacga ttcttctggg
      241 cgctgcttgc gctgctggtg ttctgcctgg cagggctggc ggtcaatcag gaaccgattt
      301 cgctcaagtt cgtcacctgg cagaccccgc aactaagcgt attctggtgg ctgctagccg
      361 cgttcagcac cggtgtgctg ttgggtctgg ttggcatctc ggtcgttcgg gcccggctcg
      421 ggctgaagaa ccggtcgctg gttaaacggc tcgaggcaag cgaacgtgag ctgcgcagat
      481 tgcgcagcgt gtcctaccac gagtaaacgc gcatgcccga gaaccttgtc gtctacctgc
      541 ttcttgtgct ggcactcggc atcggctttc ttctcgggcg acgcgagcgc aaacgcaaac
      601 agtccagtcc cggcatggtg gctcaggagt acttccgggg cctgaaccat ctcctcaacg
      661 agcgtcatga cctggccatc gacacgttcg tggaagccat ggcagtggac agcgacaccg
      721 tcgacaccca cctggcgctc ggaagtctcg tccggcgacg tggagaggtg gaccgagcca
      781 tccgcattca ccagaacctg ctcgcccggc cggtcctttc tcctgcgcac cgcgctcaga
      841 ccgagctcga gctggcgcgc gactatctgt ccgccgggct gctggaccgg gcggaaaatc
      901 tgctgctgga actggtcaag aaacccggcg acgatcgcga cgtggcgcgc acgatgctgc
      961 tcgagatcta tcagcgggag cgggagtggg agcgcgccgt ggaggtggcc cgggagctct
     1021 cccgtgccga tcgatcctat cgcacgccgc tggctcactt tcagtgcgaa cttgcggaga
     1081 aggctctggc cgatggggat ctgcggcgcg cccgcgccga tctcgagcga gccggggact
     1141 ttgatccggg ttgcgctcgg gtcgccctgg tcggcgcgaa agtcgcctcc gccggtggcc
     1201 gccaccgcga cgtcaagcgg ctgctgcgga aggctcgcag cctcgagccc gagctcagcg
     1261 gggaaaccgt ggcgatgttc cgggccgcca gtgaagagct cggggatcgt gccgatttcg
     1321 tgcgctatct gagggagtgt ctggagaggg cggccccgct ggaggtcgtt caggcgctca
     1381 cccgggagat cgaggctgat gaaggcgacg aggtggcgcg ggagtttctg atcgagcagc
     1441 tgctgcgcaa ccccaatctg gcgggcttcg tctccctgct cgagcagctc gaccgtggcg
     1501 gagcggctct cgaggccgac cagctggcgc tggttcggag gttctcgagc tcgctgctcc
     1561 agaagcagcc gagccatcgc tgcaaggact gtggcttcag cgggcacatg ctcatctggc
     1621 agtgtccgag ctgcaagacc tggggcagca tcaagcccat cgtgcgttac gacggcgacg
     1681 tctgaactcc attggcccgc gtcctcgccg ggtccgaact tacggaaacg gcggctcttt
     1741 gcggcttacg cgggcgcctg ggcgcctcgt agcattgtcg tcctcctcac cactgataac
     1801 ggagataccc atgacgatga cgatcaaacg tgcgatgggc gctgcgctgc tgctggccgg
     1861 tgtgctcgcc gggccggtgc tcgcggcccc ggagagtgtg aacatcaaca gcgcgaatgc
     1921 ggagacgctt gccgaggtgc tgaacggcgt tgggctcacc aaggcccagg ccatcgtcga
     1981 gtaccgtgag cagcacggcg cgttccgcga cgcatatgag cttgcgaaca tcaagggcat
     2041 cggcgaacgg accgtcgaga agaacgaggc gcggatccgc ctgcaggatt gaccctgcgg
     2101 cttgaggcac cctcgatgcg gcgttcgccg ggcgtcgtcc tgagttcgcc aggccaggct
     2161 ccgggtggca ggcttgcccc ggagtctggc cgctcgtcgg cgtttttgtg acgccggtca
     2221 agcgcattgt ccggagcacc tctcattgcc ggggcgcggg cggtaagatg gagcacgaaa
     2281 gccgccgatg gcgtcgcgac gaagtatgga ttctttgcag cgtttgcccc taatgcccac
     2341 ctctgccggc gcgggtaccg gcgccgacct gctcatatgg atgatcgggt gatgaagatc
     2401 atctacgtgg tcaacgaagg acggttcttt ctctctcacc gcctggcttt ggccgtggaa
     2461 gcaaaacgtc acgaggtgga ggtggtggtg gtttgcggcg ccaataccgg ggagcaggga
     2521 ctggcggacg cggggctgcg ctacgttacg gtgcccatgt cgcgaagcgg tttcaatccg
     2581 ttcaaggaac tgcgcaccgc cgccgcgctg ctgcgaatct accgtgccga aaagcccgac
     2641 ctggttcacc acgtgaccat caagccggtt ctttacggca cttccatggc gcgctggagc
     2701 ggcgtgcccg ccgtggtgaa cgccgtgccg gggatggggt tcgtgtttac ccggcgcggg
     2761 ccgcttgccc gcatccgacg aagcgtcgtg cagctgcttt accgtttgtg catgttccat
     2821 cccaacatgc gcgtgatatt ccagaacaac gaggacatgc aggcgttcac ccagcggggc
     2881 atcgtagacc ggcagcacgt tgcgctgatt cgcggatcgg gcgtcgatct ggacgtgttc
     2941 cggtgcgctc ccgagccgcc cggccccgtg acgttcgtgc tcgttggacg gatgctccgg
     3001 cacaaagggg tttacgagta cgtcgaggcc gcccgccggg tcaggcaacg gcgccccgac
     3061 tggcgattcc agctcgtcgg cgacgtggat cctggaaatc ctgcatcgct gcggcccgat
     3121 gagctgatcg cctggcagca ggccggcgag attgagtggc tcggacaacg gcgcgatgtt
     3181 cccgaggtcc tggctgcggc tcacgtggtc tgcctgccgt cctatcggga ggggctgccc
     3241 aagacgcttc tcgaagcggc cgcctgcggg cgggccatga tcgcctcgga catcgccgga
     3301 tgccgggagg tggtccgccc gaacgtgacg ggactgctgg tcccgccccg ggtcagcgag
     3361 ccgcttgccg acgccatgct gcgcctgggc gaggacgatg cgcttcgtca tcgctttgcc
     3421 cgcgcggcgc gcagcgcggc ggaggaggtc ttctccctcg aggacgtggt tcgacatacg
     3481 ttccgggtct acgacgagtt gctgctgccg tgagaagagc cgtcgtcacc ggcgccggcg
     3541 gcttcatcgg tacccacctg tgccgcgcgc ttcgtcatga gggttgggag gtggcggtca
     3601 ttacccgcgc tcacgctctc cccggatgcg agcggcttcc cgtaacgctg gcctcagccg
     3661 acgtcgagct gtcgcgggcg ctcggcggcg cacaggtggt gtttcatctc gccggacttg
     3721 ctcacggccg ggcatcgggc gcggctgagg acgagctgca tcgggtaaac gtcgagtctt
     3781 ccgcccgcct cctcgccgcg tcggaagccg ccggggtgcc ggcgatgatc tggctgagct
     3841 cgatcaaggt gctcggagat acgagcgagc ggccgcttcg accagaggat gcctatcggg
     3901 ctggggatgc ctacgcgcgc agcaaagtcg agggggaacg cgcgcttctg gaccgggagg
     3961 gcttcacgcg cgcggccatc gtgcgcccac ctctggttta cgggcccggc gtggccggaa
     4021 acttcgcgag tctcgcgcgg tgggtcggca gcggcgtccc gttgccgctc ggagcggcgt
     4081 ctgcgccgcg cagcatggtc ggggtcgaca atctgtgcag cctgatgctt cgtcttgccg
     4141 aggaaggctc cggcgtgttc cacgttgcgg atgctcgcga ccgcagcgtc gcccagctga
     4201 ttcgtgccat ggcggtgctg ctgggggttc ccgaccgctc gtggcgggtt tcgccgcggc
     4261 tgctggcggc cgtcgcctcg gcattggggc agcgcgcacg ctacactcgg ctgttcgagc
     4321 ccctgcaggt agatcagagc atgacccggg agcgccttgg ctggcagccg ccccacgatc
     4381 ccgatcagca gttgaaggaa acgatgcgat ggtttcggga acggcgacag gctacctgat
     4441 cgcggggttc gcggcctcga cggtgctcgt cttcctgatg ctccgggtcg gccgggcgtt
     4501 gaacctaatg gccgttccca atgcgcgcag ctcccacgtc gtccccacgc cgacggcggg
     4561 ggggttgggg ttcgtgatac cgctcctggt ttttttcggg atgctttacc ttgacggcgt
     4621 cgacgtggcc ctcggcttgc tgctcagcct ggttctcgtc gcggcggcca gcctctggga
     4681 cgattttgcc gagctttcgg ccggtctgcg gtttacctgt cagacggctg ccgttgcgct
     4741 catcgtctgg cagctcgatc tgccctgggc ctggtatcta ctgggattga cggggctggc
     4801 gctgctctgg cacgtcaacc tgttcaactt catggatggc atcgacggga tcgcggcggg
     4861 gcaatgcctg ctttacgtgg tgggtgtcca ggtcctcacc ctgggagcgc ccggctggat
     4921 gggcgagctt tgctggctta cggccggcgc catgctggga ttcttggcct acaactggcc
     4981 gccggcaaaa atcttcatgg gagatgtcgg gagcgcggtg ctgggcctgc tgcttgctct
     5041 gctcgcgctc cttctggcgc gcgacggcgt gctgccgctc atcgcctctc tcatactgct
     5101 tgctgggttc tggtttgacg caacgtatac gctgtgtgtc agaatggcga ccggtcaggc
     5161 gttcactcag gcgcatcgca gccacctgta tcagaagctt gcggcattca agggccatct
     5221 ctggactacc ggtgtgttca tcgccttcgg cttgttctgg ttgctgcccc ttgcctggct
     5281 gacgttgcgc gttgcggatc tggcatgggt ctggcttgtg gccgctgtgg ttcccatcgg
     5341 cgtggcgtcc atgcgtttcg gcgcggggct gcccgcccag gacgagggct gagaagatca
     5401 tcgagaattt catcaatcag ttgtcccggc gcaagcggca ggctattgcc gtggtcacgg
     5461 actttgccgt gcttcccatc gcgctctgga cagcgatcgc cctgcgcctg gggcaggtca
     5521 atccggaggt ggcgcagttc tggccggcct tcgtcgtcag cagcctgctg tgcatcccgg
     5581 ttttcggtgc tcttggcttg tatcgccacg tggtccgcca tacgggcagc gaggcgatct
     5641 gggccatcgt caagggcgcg accatcattg ccctggttgt ggccgccgtg gcctacatga
     5701 tcccgctgcc cggattcccc cgatccgttc ccatcatctt ctggctgctc accctcctct
     5761 acgtcagcgg cacccgtttc tccatgcgcg cttacttcca atggctgcag aatcgggtcc
     5821 gcctgcgcag tcccgccgtc atctacggag ccgggtcgaa gggcgtggag ctggcgcggg
     5881 tgctgagcca gcagggcgac tacctcccga tagccttcat cgatgacgat cgtgcgctcc
     5941 acaagcgcgt catcgacggg ttgaacgtct acccgcctaa atcgctcgga agggtgctgc
     6001 gtgattccgg agcgcgcgac gtcatggtgg cgatagggtc tgcggagccg gacgagcggc
     6061 gccgcatcat cgagtttctg gagccgtttc ccgctcgcgt gcgcttgatt cccgatctcg
     6121 tggagctgga gtcacgccgg cagaccatcg ccaacatccg ggacgtggag atcgaggacc
     6181 tgatcggtcg caacacggta tctccgttgc cgcaccttct tcgcggttcg gtggaagggc
     6241 gcagcgttct cgttaccgga gccggcgggt ctatcggctc ggagctgtgt cggcagatcg
     6301 ttcgtcaggg cccacaacgc atggtgctgc tcgatcagtc cgagttcggg ctgttcgaga
     6361 tccagcgcga gctaacgcag ctgatcgtat cggaagggct gcccgtggac gtcgtggcgg
     6421 tgctcggctc tgtcaccaac ggcacgctca tgcggcgcaa catggagagc tacgccgtag
     6481 aaaccgttta ccacgccgcg gcttacaagc acgtggatct cgtcgagcac aacatcatcc
     6541 aggggctcag aaacaacacc ttcggcaccc tttacacggc gcaggcggcc atggacgccg
     6601 gcgtgcgcga gttcattctc atctccaccg acaaagccgt tcgcaccacc agcgtcatgg
     6661 gcgcgagcaa gcggctggcc gagatggtcc ttcaggcgct gcaggaaata tccgacagta
     6721 cccgcttctc catggtgcgc ttcggcaatg tcctgggctc gtcgggatcc gtggtgccgc
     6781 tgtttgccga gcagatcgcc cagggcgggc cggtcaccgt cacccatccc gaggtcaccc
     6841 gctacttcat gaccattccg gaggcggcgg agctcgtcct gcaggccgcc agcatggccg
     6901 aaggtggaga cgttttcctt ctcgacatgg gcgaacccgt caagatccgg gagctcgcgg
     6961 agcggatgat caggctcaaa gggctgacgg tgcgcacttc ctctcaatcg ggtggagacg
     7021 tggagatcag gatcaccgga ctcaagcctg gcgagaagct ccacgaggag ctgctggtgg
     7081 gagacgaggt gatcggcacc gagcacgcca agatcatgcg cgccgaggag acgttcctgc
     7141 cgtggccggc gctcaggggc gcgctgaaca ccctcgaaca ggcctgcgat gcctatgatt
     7201 acgatgccat caagcagttc atcgagggcc tcgtggaagg ggcgagcctg gagagccagc
     7261 tcgggcacct gaatgccgga ccgcgcgtcc tgcagctgga ccccaaccgc gagtcgccgt
     7321 cgcaaccctg aaccggcgat ctggccacgg cctcaagcag aacgcccgcc ggccgcctcg
     7381 cggccggcga tcctcccgaa caccgaacct gacgtcagcc cggtaccacc cgggtagttg
     7441 aaatagaaca gcccgccaac cagctcgccg gcggcaaaca gcccttcgat gggctcgccc
     7501 ccctgatcga tgacccgggc tcgcgagtcg gtatgcaggc cgccgaaggt gaaggtgatg
     7561 ccgcaggtga cgccgtaggc ctcgaagggg gcggtatcca gctggtttgc ccagttggac
     7621 ttggggatgg cgagcccgac ggttccacga ccgtccttta cgctggggtt gaaaggaacg
     7681 tcggttcgaa cggcccgatt gaaggcctct accgtcgacc ggaagccgct cacgttcatg
     7741 ccttccattt tttccgcaag ggattggagg ctgtcggcga ctacccgggt gaccgcgcgg
     7801 atccggtact cgtcacgaag gagcccggcc accttggcat cgaatacctg ccaggccgtc
     7861 tgttgaggct gggcgagaat ctcggcgccg tatctggcgt aggtgtagtt tcggaagtcg
     7921 gctccttcgt ccacgaagcg ccgtcccgcg ttgttgacga gaatgccgag cgggtaggaa
     7981 tgtttctgaa aaccgtcgcc gacgctcagg tccccgtact cgggggcgtt gagatcccag
     8041 gccaccgcat gacagcccga ccagtggccg cgggctgcgg ccccgacctc caatgccatc
     8101 cggatcccgt cgccggtgtt gaagcgggtg ccgcgcaccc gggccaggtc ccagcccggg
     8161 cccaggtggc gtgcgcgcca ggcggcgttg gactcgaacc cgccgctggc caggatgacg
     8221 gcgtcgccgg caatcgtctc caggccggcc ggggtctgcg ctcggacgcc caccacgcgc
     8281 tggccgtcat ggtcgagggc taccgcgcgg gccgagtagg cgatgtccac gccgcgcccg
     8341 cgggcgacct cggtgagctt ttcgaccagc ccgggtccgc caccccaggc ctcgacggtg
     8401 agcccgcccc agaagcgcgc ctttccatcc acctcgaatg attgccgtcc gtagagagtc
     8461 aggaagcgga cgccctggtc gcgcatccac accagcgtgg aaaagctcga gtcgatgagc
     8521 gtctccgcaa gctcggggtc ggtgcggtaa tgggtgatgc ggcccatgtc gtcgaagaac
     8581 gcatcgcgcg gatagctgcc gaactcggtg cgtgcgattt ccgcgtcggt gagctccggc
     8641 atcagccgtt tcaggtcctc gacgccgtcg taggtcaccc gcatggcgcc ggccgtgaag
     8701 cgggagttgc ccccggcgag agtctccggg gcgcgttcga gaagccgaac gtcggcgccg
     8761 tgctcggccg cgctgatcgc ggcgcaaagg gccgcgttgc cgccgccgac gacgatgacg
     8821 gtgggggttc ttgccattgc tctgtcaggc cgttgcccgt agtctcgtgg agactatcac
     8881 gggaggaggc gcgaccgagc gcttcgagcg agggggagcc gcaccgttga cgttgccgag
     8941 aatgtcgttc gcgatggttc agaccgacgt gcgcaagctc gagcccatgg agctcccgct
     9001 gcctgaaatc gacgaagatt cggcgctgct gaagctggaa gcctgcggta tctgcggcag
     9061 cgatcacgag cagtacgagg gtcttctgcg cacgcccatg cccgtggttc ccggtcacga
     9121 gcccctgggc accatcgcca agatcggtga ccgggcggcg actcgctggg gcgtcgatca
     9181 gggcgatcgg gtcgcggtgg agacgatgat ctcctgccgt cactgccggc cgtgcctgag
     9241 cggctcgtat cacctctgcg accaccggct catctactcg tacgtgcctg tgagcgaagc
     9301 gccggggctg tggggcggct actcacagta catgtatctg catccgaaca gcatcgtgca
     9361 tcgggtgagc ccggagctgc cggccgagat cggcgtgatg ttcaatccgc ttggcgcggg
     9421 cttccgctgg gcggtggagc tgccggcgac ccggccggga gacacggtgg tcattctcgg
     9481 ccccggccag cgggggcttg ccagcgtgat tgcctgccgg gaggtagggg ccgggaccat
     9541 catcgtgacg ggcctttccg cagacgagaa gaagcttcag ctcgcccggg agttcggggc
     9601 ggattacacc atcgacgtgg acagcgagga cacggtaaag cgggtcaagg cgatcacgga
     9661 cgggcgcggc gccgatgtgg tcgtcgacgt gagcgcatat gcgacgaaac ccgtgtccga
     9721 tgcgctgtcg ctggttcggc cgggcggcac ggtggtgctg gccggcgtga agggcttcaa
     9781 ggcggtggag gatttcgtct cggatctcgt ggtgctcaag ga
//
LOCUS       DBNHLM010000242        28516 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_154149, whole genome shotgun sequence.
ACCESSION   DBNHLM010000242 DBNHLM010000000
VERSION     DBNHLM010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 28516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 28516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28516
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_154149"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /gene="acnA"
                     /locus_tag="AC16QF_11115"
     CDS             complement(<1..1559)
                     /gene="acnA"
                     /locus_tag="AC16QF_11115"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR6449150.1"
                     /translation="MSLDTFKTRRTLTVGGREYDYFSLPEAQSAGAGNVEKLPMSLKI
                     LLENLLRFEDDNTVKVEDITALANWASGGAAAEEIAYRPARVLMQDFTGVPAVADLAA
                     MRSAVVSAGLDPTIINPLSPVDLVIDHSVMVDSFGTETAFAENVALEMQRNTERYRFL
                     RWGQKAFDNFRVVPPGTGICHQVNLEYLARTVWTREENGRTVAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPISMLIPEVIGFRLTGKLRDGITATDLVLTVVEMLR
                     RRGVVGKFVEFFGEGLDQLPLADRATIANMAPEYGATCGYFPIDRETIAYLELSGRPA
                     ETVELVEAYARANAMWRDADTPEATYTDVLALDLDSVVPSLAGPKRPQDRVAMKDLGR
                     VFDEALELQGRAGDSDARVPVPGTDYDLGHGDVVIAAITSCTNTSNPAVMLGAGLVAR
                     KALARGMQVKPWVKTSLAPGSKVVTEYLEKTAVQEDLDALGFNLVGYGCTTCIGNSGP
                     LPEHISAAIKQG"
     gene            1732..2175
                     /locus_tag="AC16QF_11120"
     CDS             1732..2175
                     /locus_tag="AC16QF_11120"
                     /inference="COORDINATES: protein motif:HMM:NF016701.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6449151.1"
                     /translation="MKVDGRCFCGAVTYDAEIDPDRVLICHCTDCQNHSGSAYRVVAG
                     VVNDSFRLLTGELRSYEKVADSGSLRSRTFCPECGTNIYACTVGEGTGFFGLRLGTIN
                     QRAELTPKLRVWCRSALPWSDDLSGLPAFPAQPDLDALDEIVHQS"
     gene            complement(2191..3018)
                     /locus_tag="AC16QF_11125"
     CDS             complement(2191..3018)
                     /locus_tag="AC16QF_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0034702 - monoatomic ion channel complex
                     [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HIR6449152.1"
                     /translation="MALDRDTVRVIIFEAHTPAGKAFDVGLIVCILVSVLAVMLDSVG
                     AIHHEYQDVLYGVEWFFTILFTVEYGLRLWCIENTGRYARSFYGIVDLLGIIPTYLSI
                     FFAGTQYLLVIRVLRVLRVFRVLRMMRYVGEAELLTQALRASRRKIIVFISSVAALVI
                     IFGSMMYLIEGEANGFTSIPRSIYWAVITMTTVGYGDITPQTPLGQAVASLVMIMGYG
                     IIAVPTGIVTLELSEASRRAMNTRTCPGCSAEGHTGEATYCWHCGHHLFEPRAVAHD"
     gene            complement(3064..3480)
                     /locus_tag="AC16QF_11130"
     CDS             complement(3064..3480)
                     /locus_tag="AC16QF_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR6449153.1"
                     /translation="MSIVRWNPWREFDDFFTNVAAVPSEHIARSEWLPPVDISETETE
                     YLIDVEIPAVARDDVAVSVKDGVLTVTGERNFEKETDGKRHRVERQYGRFSRSFRLPE
                     NVDEEHISASSKEGVLYLVVSKREKEKPRTIEVKVH"
     gene            complement(3634..4293)
                     /locus_tag="AC16QF_11135"
     CDS             complement(3634..4293)
                     /locus_tag="AC16QF_11135"
                     /inference="COORDINATES: protein motif:HMM:NF017534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6449154.1"
                     /translation="MQIRFWRDRRPWRPVSMHQSAPNPCLERHWGGLRLEHGSTVFVP
                     MCGKSDDLRWLLGRGHQGLGIELADLAIEAYFEEAGEPYEREDSDRLVRYRGPRTDIL
                     CGDFFALTAAELSAARAVLDRGALIALPPKMRARYVDHLLRVIPDRTDILLVTLEYDQ
                     NLVAGPPFSVLPEEVVSHFGPRCSIVPLESSRTDFLPPKFAAQGVDCAAETAYHIVKQ
                     E"
     gene            4531..4956
                     /locus_tag="AC16QF_11140"
     CDS             4531..4956
                     /locus_tag="AC16QF_11140"
                     /inference="COORDINATES: protein motif:HMM:NF017913.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HIR6449155.1"
                     /translation="MMQLIANIERWVVLGLLVLMLLVVVASSIELAIIIVQAALENHF
                     SEEPAYLLLDINELLGIFGFFLLVLIGLELLASVKMYLEDDIVHVELVMLIALIAVSR
                     KIIVIDYAKVDGVTVLGIAALVLVLSVGYYLMKRSHEPS"
     gene            5148..6008
                     /locus_tag="AC16QF_11145"
     CDS             5148..6008
                     /locus_tag="AC16QF_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449156.1"
                     /translation="MIDAAVRRFDAFFLTEAQETFEEDRNRLRLRLDTDYIEHHGWEF
                     SPRVKVNFHLPGLGERVRLVVNDAGGDEEDEGVELEDDENDVALRWVARKGKKTGISV
                     DGGVSFRGGEVDPFVRVNTSYKYRISENWRGQATNRLYYLARNGWRDDLRIYFNRPIG
                     ERFLFRSRSRLEYFEDKDSNPQLEQKFSLFQNISDRSAIAYEAIWRQDTAEDSIYDDE
                     EILIGPRDKYQQVKLRIRYRRNFWRPWMFVEFWPVVGWVEERDWDTVLGARMRLEINF
                     GWHAGSKLDE"
     gene            6001..7263
                     /locus_tag="AC16QF_11150"
     CDS             6001..7263
                     /locus_tag="AC16QF_11150"
                     /inference="COORDINATES: protein motif:HMM:NF028178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR6449157.1"
                     /translation="MSKREPGLAAFAALMLGFTGTNLAGCSPSVTEVDLIMPELPVEQ
                     IIARKIADLVDEDSGLKINLIPLPDDSLSVLDALEQGYGDIAFAPNTNEHRDTITTIM
                     PIYPSVLHVGSIADRADGSLEELLDGARVFAGPPGSIPRQIGERIVSGLQLEKGLSFT
                     SDQDAAPDVIILFAPIDRRRVMSNPSLESLKLFSFGDPGDTRPGSIVDRTVLLNPTLR
                     PYVIPAGTYGSLTPDPVVTFAVDTLLVARESLDETLVFDLFEEFLRLRPALFSKRPEL
                     FQPIDDDIARAHWTFSLHPGALDYLQRDEPSLIERYSGVAEVLVTLLVGMVSGAFAIL
                     KIYQVRRKNRIDAFYTQVIRTRDSVAEGAGSEERAAALAQLRSLRERAYEMLVEEQLA
                     ADTSFLIFVLLTNDAIAQIDSPENGPSV"
     gene            7340..8209
                     /locus_tag="AC16QF_11155"
     CDS             7340..8209
                     /locus_tag="AC16QF_11155"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR6449158.1"
                     /translation="MAYPRPELLEEPAALAARLDDADLRIVDATVFLVPAERGYRAES
                     GRERYEQGHIPGAAFMDLIDAFSDTTTGLGFSLPDPGVIADALGRLGISDDSEVVVYS
                     TGHLMWATRAWWLLRYVGHQRASVLNGGFDAWRDLGLPFETGTARRAAVTYRPRPDAS
                     LFTDRDGMCRAMDEPGVCTVNALSPEVYAGTGTTNYGRPGHIPGSVNVPYEELLAGGR
                     FKPAGALAEALESKGLLDADRVVTYCGGGIAATLDGFACLLLGHDNVAVYDGSMSEWA
                     RDPSLPLNVGEAP"
     gene            complement(8295..8999)
                     /locus_tag="AC16QF_11160"
     CDS             complement(8295..8999)
                     /locus_tag="AC16QF_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6449159.1"
                     /translation="MTAPGDATVRLLNDLFGGGVKNAVVLMRHSAREFAPGRHDLDNP
                     LTEEGRDLARRLGERLPEDLTVRAYASPPERCMETAELVLAGYASTGGRVTRHRPVEA
                     LGVFYALDQMKMWQVMQSAGGMVPFLEAWFSGDVPQDAVMPPRLAAALVVRLMTDKLS
                     AMDGARRLDVCVSHDMTLHLVRDRLLGQSVRDVDVDFLDGLVAFEREGARWLASHHGE
                     PVKLDAQLLSLGGLWD"
     gene            9124..10356
                     /locus_tag="AC16QF_11165"
     CDS             9124..10356
                     /locus_tag="AC16QF_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449160.1"
                     /translation="MDLGISERVKPLIDDITQYIHDYVVPNERAYAEQVDAGGRWTET
                     EAMEEMKEKAKERGLWNFFLPDSERGSGLNNLDYAHVAEVLGRYPLSSEAMNCAAPDT
                     GNMEVIERYGSEQQKEEWLRPLLEGKIRSAFAMTEPQVASSDAANISLEARLEGDEWV
                     LNGEKWWTSGIGDPRCKIMIVMCVTEPDAPRHSRQSQILVPVETDGVEVLKMMHVFGY
                     DDAPHGHGHVRFTDVRVPKDNIILGSGRGFEIAQGRLGPGRIHHCMRSIGVAERALEL
                     MCRRALSKEAFGKPIADLGGNYDKIADARINIEMARLLTLKAAYMMDTVGNKIARSEI
                     AQIKVAVPNIALKVIDDAIQIHGGAGVSQVFPLAKMWAGQRTLRLADGPDEVHRRAVA
                     RLELGKYPDKDPIRTDVW"
     gene            10369..11283
                     /locus_tag="AC16QF_11170"
     CDS             10369..11283
                     /locus_tag="AC16QF_11170"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6449161.1"
                     /translation="MGRLDDKVAVITGAGRGIGREIALMMAGEGAKIVVNDLGGNMDG
                     TGTGMIADDVVAEIRAAGGEAVSNTASVAEVAGGESLLQSALGAFGAMDILVNNAGIL
                     RDRTIFKMEEADWDAVQAVHLKGHYCCTRPFARYIRENNRTGCRVLNFSSVSGLYGNF
                     GQANYGAAKAGIAGFSRVLALELAKYGCTVNTISPGALTRMTIPLREARGEEVKDEEI
                     SESGPQHVAPICVWLATEAAAGITSQIFHTARGGVGIMQQPRVIKQYHKSSGLWTLDE
                     LDGIVPELVAARAANEAAAKDSGQPIEV"
     gene            11433..11771
                     /locus_tag="AC16QF_11175"
     CDS             11433..11771
                     /locus_tag="AC16QF_11175"
                     /inference="COORDINATES: protein motif:HMM:NF024901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR6449162.1"
                     /translation="MGIQRAAIMIIVGALAWATANGAYAGKIVSWVDEKGVTHFTDAG
                     LANGQTNAVTVATSDTPVRAAPEDIVPERKSTPTVAQSGSGKSEGQKSGLRGYRSSWA
                     RDPDRFKNLN"
     gene            complement(11823..13082)
                     /locus_tag="AC16QF_11180"
     CDS             complement(11823..13082)
                     /locus_tag="AC16QF_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449163.1"
                     /translation="MWRRHRSWLIGPFLGLWATGSAAQSDFSARVKWFGTATALPEHD
                     LQRNLSGTPAYDQNYDLRLMFQKTISAVTLQVEHSTTLVTGDSLNFPTAPGTVLEQSP
                     EGDERRLMDLTWEIEDGDRHRSFHRLDRLALKLRRDDWAVTVGREAVSWGAGLVFRPM
                     DLFNPFAPTAVDRDYKAGDDLLLIERLFSDGSDLQVLAVGRRDETGDFTGQAGSLAGK
                     WRGFIGACEVELMAAKHYRDQVYGIGVRYPLKGAMLRTDLVATRLADGGVRVGGVVNL
                     DYSLEVAGRTTYVFAEYFHNGFGVSELPETVEELPAPLLDRLGRGELFNVMRDYLAGG
                     VTYQWHPLLVQTVTLIGNLNDGSLLLQTQINFDPGDNQRLELGVVEPLGGSGDEFGGV
                     PVVADPAGSRLTVGGGSRLYARWVYYF"
     gene            complement(13066..13767)
                     /locus_tag="AC16QF_11185"
     CDS             complement(13066..13767)
                     /locus_tag="AC16QF_11185"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6449164.1"
                     /translation="MAERVVRCIGVSRTYQDDSVPVHALRDVDFEVGKGEFVTLAGPS
                     GSGKSTLLNMIGGLDRPSSGCVEVDGVDLGSLSESRLSDLRLRKIGFVFQSYNLIPVL
                     SARENVEFIMQLQGVSAAERAARARAALEALGIGELEKRRPGELSGGQQQRVAVARAI
                     VTEPVLLLADEPSANLDSATTRELLEYLRRLNQDRGVTIVAATHDPMVMGYAQRQVGL
                     RDGAIVKDSADVAPA"
     gene            complement(13775..14983)
                     /locus_tag="AC16QF_11190"
     CDS             complement(13775..14983)
                     /locus_tag="AC16QF_11190"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6449165.1"
                     /translation="MLKLLFQLSARNLLRHKRRNLMLLMAVVVAVGGVTLANALIRGF
                     QHDMREAAVQNLTGHLKVLAPGYLDDPGIQRSFTLVQGWSPDIGPRLLQGWAARIRIP
                     AVIMSERETRGVQFVGVDPAQETISFLGDASYRGEPLSGAADRRVVIGQALAEQLDTD
                     VGRRLVLITQGADGLNREAGFRIAGVYDADGQGLEKMFVFTGVTRLQELVDANVFTEV
                     SIKLREEPRGDGLKQGLAETFTDLDVMDWKELEPQTAAMFLFADTAIFIWFCIMMGAL
                     IFGLVNALITAVMERIREFGMLRAVGMRPGSVVVQVVLESTLIMAAGVALGLGLGFLL
                     VVWLSDGIDLTEWAAGVELFGMRSVLVPRLLLEDVVLVAAMSIVFGLIASLYPAWRAV
                     QIGPLDALRR"
     gene            complement(15072..16340)
                     /locus_tag="AC16QF_11195"
     CDS             complement(15072..16340)
                     /locus_tag="AC16QF_11195"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6449166.1"
                     /translation="MTQARTPAAGGRARPALLLRIGWRNLARNRRRTWLTAGGIAFAV
                     LLVVFSMAMQLGQYDIMMENATSLLTGHIQIQTADYVDSARFEDTMGSAGALRRVIEA
                     TAGVEAVAPRAEAFALASAGERSFGARIIGVDPDAERDTVRLLKMVREGRAIAAPDDG
                     LLGSLLARNLGVTLGDEVVLLGSEKHGGVAAMVITVAGILETGVADLDRVTLITPLAS
                     VQSAFGLADEVHTFAIRTEDLDRSEAVAESLRHSLTDRPALVVRHWSEVMPELRQAIE
                     IDRLGGELFFYVVEVLVVFSIVNTFIMTVFERTREFGMLKAIGMKPWRIVAMMQWEGF
                     FIWLLGASLGLGLAAIIVAWLQDVGIYMGETLEEYAEQFYMPARLYPAFSLQAFLSAP
                     LVMLMGTQIAALLPSLRIFRLNAAAALRGE"
     gene            complement(16337..17110)
                     /locus_tag="AC16QF_11200"
     CDS             complement(16337..17110)
                     /locus_tag="AC16QF_11200"
                     /inference="COORDINATES: protein motif:HMM:NF028441.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HIR6449167.1"
                     /translation="MHSVKRLLLTAAHSMLVIFPAGVLAAEEPTARELVAAAIDLTRG
                     QSSYAEFAMVIHRPDWQRTSKLVAWTRGRKDALIRFTAPARDAGNATLKQGEKMWTYN
                     PKLNRTIRLPFSLMSQSWAGSDFSYNDLSRTDKLLEEYELAITATRRSEGHTLYTIEA
                     VPREEAPVVWGKEEIVLRDDHVLISQTFFDQAMRPLKRMETLEIGEMGGRIFGTRMRM
                     SKLDEPENYTELSYARVDFDIELEDRIFTLFSLQSGQRP"
     gene            17183..17797
                     /locus_tag="AC16QF_11205"
     CDS             17183..17797
                     /locus_tag="AC16QF_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6449168.1"
                     /translation="MPLREEKKRQVRKDILDAARELIEQRGYDSSTMRDIAGTARVSY
                     QTLYNYFPTKAAILAALLNDVVEQAVADIEALIEGYDGRLVETLNAINRARIDLVARR
                     DRELWRIISVDRLRDGQDAGAVHQRLDDAAGERLKQLLALARQRGELVPDVDVGLLSD
                     TLKALGERAVARYLEASAASRRSVAESLAAQTSLLVTPYLEPAR"
     gene            17794..19803
                     /locus_tag="AC16QF_11210"
     CDS             17794..19803
                     /locus_tag="AC16QF_11210"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6449169.1"
                     /translation="MTIRWGILATGSIAHTFARAVKLSATGRLQAVGSRRGESAEAFA
                     RALDVPSAHGTYAELVNDPEVDAVYIAAPHPDHAEWSIRALRAGKAVLCEKPAGLNHA
                     EVMAVIDEAEQRQAFFMEAFMYRCHPQTLRLVELVREGAIGEVRHIEARFGFHADFDP
                     ASRLFGNALGGGGIMDVGCYPVSMARLIAGAEPDQVSGHGKVGASGVDEWAAALLKFP
                     NGLSAQVASSVSLALDNSVRLNGSAGQIIVKNPWHCADAEGRWALQIRRRGRDPETVE
                     GTAPPLYELEADHVADMIAQGRSESPHMSWTDSRNNALVLDAWRREVGVEFQRETKAA
                     PLLGGPLRRPERPPMTYGRVANLSKPVARLVMGCDNQPSLSHAAIMWDHYFERGGNAF
                     DTAYIYGGGSMEKLLGRWHTSRRVRDDIVIIGKGAHTPDNRPEAVSRQLDRSLDRLQT
                     GEVDVYFLHRDNVDIPVGEFVSALNDEAAKGRIKAFGGSNWTLARVKAANAYAAQQGL
                     QPFTVLSNHFSLARMVEPIWPGVEAASDPEFRAFLAASKIALFPWSSQARGFFTPWAD
                     PIIADRGAENRAVTTMEPTIAELKRTWFAQDNFERRARAGAVAERLDVEMINVALAYV
                     LNQPFPCFPLIGPRLPWETRSSLAALEVALSDAERAWLDLSADGG"
     gene            complement(19784..20245)
                     /locus_tag="AC16QF_11215"
     CDS             complement(19784..20245)
                     /locus_tag="AC16QF_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449170.1"
                     /translation="MILIRSGQWTGRGSILAEGQSLGQGVACDVEVQRDDEGASISAE
                     VDAKELGSWAMNVRIAPDEQGTYDLSVRTLKGSLTGTAKLDSPPNLGLLWNEEQTVHA
                     TFTLFEIAGGLGFRGFLRDGKTTYTWEIALSLRQDHIGGENVVSLVSRRRR"
     gene            complement(20242..20940)
                     /locus_tag="AC16QF_11220"
     CDS             complement(20242..20940)
                     /locus_tag="AC16QF_11220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HIR6449171.1"
                     /translation="MCGRFNVTSSPLSELLLELVGLPHPGPDNYNAAPTETIVVLRMN
                     DDGRPELVAMRWWLTPFWSKSVTTKYSMFNAKSETVEKSPAFREPFSRRRCVVPVSGF
                     YEWARSKERKLPYYLTPHLASGMLLAGIWDSWQDGENGPLLSFAVLTCEANEQLTFVH
                     KRQPVMLGMKDARIWLDPSTQASALKALCAPRVPVALDAVPVSSYVNNARNKDSRCTE
                     AIGTAVAIREDVTA"
     gene            complement(20952..21599)
                     /locus_tag="AC16QF_11225"
     CDS             complement(20952..21599)
                     /locus_tag="AC16QF_11225"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6449172.1"
                     /translation="MAHVQPRSIDDVPALKPIFDAAESAMGFVPNSMLTMAHMPQLPM
                     GFMMLTNVIFGGDLKAMADQLQPLIPEQGDAGENLSPPLIQLIAFATSVSSGCRYCQA
                     HTAHSGHRQGAEQEKFDSVLDYENSPVFSDAERAAIGLALAAGAVPNQADAMHFERLR
                     AYFSERQIVQIVAVIAIFGFLNRWNDTMATQLEAPAVDYAAASLGELAWAIGKHG"
     gene            21698..23797
                     /locus_tag="AC16QF_11230"
     CDS             21698..23797
                     /locus_tag="AC16QF_11230"
                     /inference="COORDINATES: protein motif:HMM:NF014896.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR6449173.1"
                     /translation="MIRPPVAKRRNHRHTHHGITLEDAYYWLRDPAYPDVKDPEIIEY
                     LTAENAYFDARMEAHQPLVTQLFEEIKGRQPAEDASVPHRHGAFWYQWRYQKDAQYRQ
                     WLRAPAPAGSSPDAGPWEVILDEPELAREHPYFSLGGISVSRSGRFIAWSADTSGAER
                     FTLRIRDLQTGELVDDPIEQTQGSPVWAADDTCFFYVVVNDQWRPYQVRRHKLGDPPD
                     ADAVVYEEPEDSFFVSVDETQSEAYILIAAGDHVTSEIRYVPADEPLAEPALIARRRA
                     GHEYEVDHREGAFYVVTNDAHKNFRLARAPEDDPVEQNWQTVIEGSESHYLTGLLCFK
                     DHLVVCERIDGLDQIRIRSGEEEHHVVFPESAYAVGLGTNAEYDSATLRLHYESMVTP
                     STVYDYDIARRKLIQRKVREIPTGYDRTRYETARRTVTARDGAQVPLSIVYPRDFPRD
                     GTAPVHLYSYGAYGHAMSPTFSPSRLSLLDRGFAFAVAHVRGGDDLGRQWYEDGKLDK
                     RTNTFNDFVDCARHLIDSGFSSPGLVVISGGSAGGELMGAAINAAPTLWGAVVAHMPF
                     VDVLSTMLDAGLPLTPIEWPEWGNPIEDKAAFELIRSYSPYDQLAPGAYPPILVTAGL
                     NDPRVTYWEAAKYVAKLRHLKTDENWLLLKTNMSAGHSGESGRFDSLRELAEEYAFFL
                     VSLEAAGWHPRGEDDQA"
     gene            complement(23788..24030)
                     /locus_tag="AC16QF_11235"
     CDS             complement(23788..24030)
                     /locus_tag="AC16QF_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449174.1"
                     /translation="MDYVGIDPAVGLECGHCKHISRLSYSGLLDLVLECERAECDGCG
                     RLMMHDWTTVTVVQNIIKKRMRQANEAKVRHLVHHA"
     gene            complement(24191..25408)
                     /locus_tag="AC16QF_11240"
     CDS             complement(24191..25408)
                     /locus_tag="AC16QF_11240"
                     /inference="COORDINATES: protein motif:HMM:NF024732.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HIR6449175.1"
                     /translation="MKELSALLEPGQRIFVAGSSNEPTGLLEVLADQTLPPEIEFLQF
                     PLAGYNRFDFTAFSEDAIFTTFFMTPHLRDADPARVRFLPMQMRAVYDFLAEGVDVVL
                     LQVARNASGQLCVGPNVDFVEAAMMGAKTVAAELNHGFVAPAGCPVVDAGRIDFLMET
                     DRSLFEMAPPEIDGAAARIGEHVAQLVRDGDCIQTGIGSIPAAILARLGDRNDLGMHG
                     GLIDDGGMALIRNGNMTGVNKPIDTGKHIAGMALGSRQLLDWLAQTPAVQFRGANHTH
                     EVNVIRELPQFLSVNSAVEVDLFGQVNAEVAGGRQISGTGGSVDFMRAAKASPGGRSI
                     VAMNATARGGKVSRIVPKVELVTALRTDVDIIVTEHGVAQLRALSAAERVRALIEIAA
                     PEFRDGLRKVASG"
     gene            25559..26326
                     /locus_tag="AC16QF_11245"
     CDS             25559..26326
                     /locus_tag="AC16QF_11245"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF044908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6449176.1"
                     /translation="MREARSRVYGGQFLGQGICAASQIGHGALRSFHGYYLRPGATDL
                     GIGYQVERLGDMVVGVSAVQREKQLFLMMARFGPFCPAPPEEMPDVPPPEDCIPRERG
                     IAGLDRDTDSTWAVTDSPFDNRFVEDIWNEDYRRPLHHVWFRLRDPGALPGHIDQIVM
                     QAIAAYYADDTIMDNALFPHGWHRCWSELQTASLDHAMWFHRPMDLRRWHLFAQDSPV
                     ASGGRAMTRGTIFDDRGAVVASAAQEILLREPPDDKP"
     gene            26323..>28516
                     /locus_tag="AC16QF_11250"
     CDS             26323..>28516
                     /locus_tag="AC16QF_11250"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6449177.1"
                     /translation="MTPLAQFEPGVRAWFTERFPSPTAVQAQSWPRIAAGEHVLITAP
                     TGSGKTLTAFLWALNQYASGSWVPGHTRVVYISPLKALNNDIRRNLLEPLRSLREDHE
                     FPPVSVQTRSGDTPQSERQRMLRRPPDILITTPESLSLMLTTTRGRLALGSVETVILD
                     EIHSIVDNRRGAQLMTSLERLTDLCGEIQRLALSATVRPLEGIAEYVGGYTADGAPRR
                     VSIVDPGSAKEIEFRVRYPDEVRLALENGRKIWDPLSEVFRRQIEHNASTLFFTNSRR
                     LAEKITLKINEDQAGPIAYAHHGSLAREIRTEVEERLKAGSLKAIVATSSLEMGIDIG
                     HLDEVVLVQSPPSIAATLQRIGRAGHGVGETSVGTLYPTHANDFLEAIVLAKAVAGGD
                     IEPLTPQENALDVLAQIIVSMCASESWQLDDVFALLRRSTPYHALPREQFDLLIDMLA
                     GRYAGSRVRELKPRIVLDRINNTIRAHKGAVLALYNSGGTIPDRGYYQLRHADTGAVI
                     GELDEEFVWEATTGQIFSMGTQNWQVTRVTHNDVLVRSAPPGTTAPPFWRSESYNRSF
                     HFSERIARELEQAEALLDDGRNDDLRSTFTSAGGFEPEAAVELLDYLTRQREASGAPL
                     PHRHHLLLELVRSGPGGYRGPDDTRQLVLHTFWGGRLNRPWALALEAAWQDALGRRAE
                     IHADNNAIVIQVKGEPDPARVLAMVTADNLDELLRRSLERSGFFGARFR"
BASE COUNT         5348 a         9431 c         8921 g         4816 t
ORIGIN      
        1 ccttgcttta tggcggccga tatgtgctcc ggcagaggcc cggaattgcc gatgcacgtc
       61 gtgcacccat agcccacgag gttgaacccg agggcatcca gatcttcctg gacggccgtc
      121 ttctccagat actcggtaac gacctttgaa cccggcgcca gcgacgtctt cacccagggc
      181 ttgacctgca tgccgcgagc gagcgccttg cgcgctacca ggccggcacc cagcatcacc
      241 gcggggtttg acgtgttcgt gcaggaggtg atggcggcga tcaccacgtc cccgtggccg
      301 aggtcgtagt ccgtgcccgg gaccggaaca cgtgcgtcgg agtcacccgc gcggccctga
      361 agctccagcg cttcgtcaaa aaccctgccc agatccttca tggccacacg atcctggggg
      421 cgcttgggcc ccgccaggct cggcaccacg gagtccagat ccagggcaag aacgtcggta
      481 taggtggctt ccggggtgtc ggcatcccgc cacatcgcgt tggcccgtgc gtacgcctcg
      541 acgagctcca ccgtttccgc cggccgaccc gaaagctcga gataggcgat ggtctcccgg
      601 tcgatgggga aatagccgca ggtggcgcca tactcggggg ccatgttggc gatggtcgcc
      661 cggtccgcga gcggaagctg gtccaagccc tctccgaaga actcgacgaa tttgcccacg
      721 acgcccctgc ggcggagcat ctcgaccacg gtgagcacga ggtcggtggc cgtgattccg
      781 tcgcgcagct tgccggtgag ccggaacccg atcacctcgg gaatcagcat ggagatcggc
      841 tgccccagca tggcggcctc cgcctcgata ccgccaacgc cccaacccag cacgccgagg
      901 ccgttgatca tcgtcgtgtg gctgtcggtg ccgaccagag tgtcggggta ggccacggtg
      961 cgcccgttct cttcccgggt ccagacggtt ctggcgagat attccaggtt cacctggtga
     1021 cagatgccgg tgcccggcgg aaccacgcga aaattgtcga acgccttctg gccccagcgc
     1081 aggaaccggt atcgctcggt gttccgctgc atttccaggg cgacgttctc cgcaaacgcc
     1141 gtctcggttc cgaagctgtc gaccatcacg gagtgatcga tgaccaggtc cacgggagac
     1201 agcggattga tgatcgtggg atccaggccg gcggacacca ccgcgctgcg catggcggcc
     1261 agatccgcca ccgccggcac acctgtgaag tcctgcatga gcacccgcgc cgggcgatag
     1321 gcgatctcct cggccgccgc cccgccgctt gcccagttgg ccagcgccgt gatgtcctcc
     1381 accttgacgg tattgtcgtc ctcgaacctg agcaggtttt ctaggaggat cttcagcgac
     1441 atcgggagct tttcgacgtt cccagccccg gcggactgcg cttccggcag gctgaaatag
     1501 tcatattccc taccgccgac ggtgagcgtc ctgcgggtct tgaaggtatc taagctcatg
     1561 gctgcctcgc tcgttgtttt cggtgccagg aaaaaggggc ggtattgtac tcgttacccg
     1621 gctcctgagc agtgttttgt agggcgcgaa gcggggcgta aacccgtgga tacgcccgca
     1681 gcgcgcagct ctggtagtct tggcgggaca ttcgcctggg aggatgcctt catgaaagtc
     1741 gacggacgct gcttctgcgg cgctgtgacc tacgacgccg agatcgaccc cgaccgggtc
     1801 ctcatctgcc actgcaccga ctgccagaat cactcgggca gcgcctaccg tgtcgtggcc
     1861 ggagtggtga acgacagctt ccggttgctg accggcgagc tgagatcgta cgagaaggtt
     1921 gccgacagcg gctcgctccg ctcgaggacc ttctgccccg agtgcggtac caacatctac
     1981 gcctgcacgg tgggcgaagg taccggtttc ttcggtctca ggctgggcac catcaaccag
     2041 cgggccgagc tgacgccgaa gctcagagtc tggtgccgct cggcgctgcc atggtcggac
     2101 gacctgtcag ggttaccggc cttccctgcc cagccggatc tggatgccct ggacgagatc
     2161 gtccatcaga gctgacctcg acccgtgcgg tcaatcgtgc gccacggccc gcggctcaaa
     2221 aagatggtgt ccacagtgcc agcagtaggt cgcttctccc gtgtgccctt cggcggagca
     2281 tcccggacat gtgcgcgtgt tcatggcacg acgtgaagct tcgctgagct ccagagtcac
     2341 gatgccggta ggcaccgcaa tgatgccgta acccatgatc atgaccagag aggcgactgc
     2401 ctgacccaga ggcgtctgcg gcgttatgtc gccgtatccc accgtggtca tggtgatcac
     2461 ggcccagtag atactgcgcg ggatgctggt aaagccgtta gcctccccct cgatgagata
     2521 catcatggag ccgaagatga tgaccagcgc cgcaaccgaa gagatgaaaa cgatgatctt
     2581 ccgacggctg gcgcgcaggg cctgggtcag cagctccgct tcgccaacgt agcgcatcat
     2641 gcgcagtacc cggaataccc gcagcacccg caacacccga atcaccagca gatactgggt
     2701 gccggcgaag aagatgctca gataggtggg gatgatgccg aggaggtcga cgataccgta
     2761 gaagctgcgc gcgtaccggc cggtattctc gatgcaccac aggcgcaggc cgtactccac
     2821 ggtgaatagg atggtgaaga accactccac gccgtagagc acgtcctggt actcgtggtg
     2881 aattgccccg acggaatcga gcatcaccgc gagaacgctg acgaggatgc agacgatgag
     2941 gcccacatcg aacgccttgc cggcgggcgt gtgggcctcg aagatgatga cccggacggt
     3001 atctcggtcg agcgccatgg cacgtattaa accacggcgc gacgtcgggt cagcaagcca
     3061 tcgtcagtgg acttttacct cgatggtgcg cggtttttcc ttctcccgct tggagacgac
     3121 cagatagaga acgccttcct tggacgaagc cgagatgtgt tcctcgtcca cgttctcggg
     3181 cagccggaag ctgcggctga atcgcccgta ctgccgttcc acccgatgac gcttgccatc
     3241 ggtctccttc tcgaagtttc gctcgccggt taccgtcagc acgccatcct tcaccgagac
     3301 ggcgacgtcg tcccgggcaa ctgccgggat ctcgacgtcg atgaggtatt ccgtctccgt
     3361 ttcagagatg tcgacgggcg gcagccactc cgaacgagcg atgtgctcac tgggcaccgc
     3421 ggccacgttg gtgaagaaat cgtcgaattc ccgccagggg ttccatcgga caatgctcat
     3481 ttcagttggc tcctaaccac tcagttcaaa caacgattcc cgataaatgg gcgctgccgg
     3541 aaaatttaaa agcccttgaa aagagaaaat tgatccccag acggcaacaa aactgctgct
     3601 cgccgaccgt cgaccggccc gggatgggga acgctattcc tgcttgacga tgtggtaggc
     3661 cgtttccgcc gcgcagtcca caccctgagc ggcgaatttg ggcggcagga agtcggtgcg
     3721 ggaactctcc agcggcacga tgctgcagcg cggcccgaaa tgggaaacca cttcctccgg
     3781 cagcaccgag aacggcgggc ccgccaccag attctgatcg tactcgaggg tgacgagcag
     3841 gatgtcggtg cggtcgggaa tgaccctgag caggtgatcg acgtagcggg ctcgcatctt
     3901 cggcggcagc gcgatcagcg cgccgcgatc caggacggcg cgcgcagcgg agagctcggc
     3961 ggccgtcaac gcgaagaagt cgccacagag gatgtccgtt cgaggaccac gatagcggac
     4021 gagccggtcc gaatcctccc gctcgtaagg ctccccagcc tcttcgaagt aagcttcgat
     4081 agcgaggtcc gccagctcga tgccgagacc ctgatggccc cggccaagca gccatcgcag
     4141 gtcgtcgctc ttaccgcaca tgggcacgaa cacggtggag ccatgctcca gcctgagacc
     4201 gccccaatgc ctttccaagc acgggttagg cgcgctctgg tgcatgctga ccggccgcca
     4261 gggccggcgg tctcgccaga agcggatctg catcgttatt cttctacacc gccaggcgct
     4321 gcgcccccgg cgatgtttac ctccctgttg accgcattct ggccaatgcc gtcgcctgcg
     4381 gcaaccactt tggggcccgc gcagcggatc cacggcggtg gtttctccgg ccgctgcccg
     4441 ccaacgccga cggacgccga ttggacatcg ccccgggccc tctctaatct aatcgtcgcc
     4501 ctggctaagc ggcgcgggat ggcttttcat atgatgcagc tcattgccaa catcgaacgc
     4561 tgggtggttc tcggtctact cgtgctcatg ctgctcgtgg tggtggcgag ttcgatcgaa
     4621 ctggcaatca tcatcgtcca ggcagccctt gagaaccact tttcggaaga gcccgcctat
     4681 ctccttctcg acatcaacga gctgctcggc atcttcggtt tcttccttct ggtcctgatc
     4741 gggctcgagc tgctggccag cgtgaagatg tacctcgaag acgatatcgt ccacgtggag
     4801 ctcgtgatgc tgattgctct gatcgccgta tcgcgcaaga tcatcgtgat cgactacgca
     4861 aaggtcgacg gcgttaccgt gctgggtatc gccgcgctgg tgctcgtgct ttccgtgggc
     4921 tactacctca tgaaacgcag ccacgaaccg tcttgagatt cccctggact tcagtctcga
     4981 caatcctcct cgccgggctc atggccctgc cgctgagcgc atcggccgag cccgttgact
     5041 catctgccca acccggtcgg acggcgaacg acaccccccc gcccgacgcg gatcaggtcg
     5101 agccaggacc gacaaaagta gacgatcttc acacatcgac cgcagggatg atcgacgccg
     5161 ccgtgcgccg gttcgacgcg tttttcctga ccgaggccca ggagacgttc gaggaggatc
     5221 gaaatcgcct tcggctgcgt ctggacacag actacatcga gcatcacggc tgggagtttt
     5281 cgccccgggt caaggtcaac tttcatcttc ccgggctcgg tgaaagggtc cgcctggtcg
     5341 tcaacgacgc tggcggtgac gaggaggacg agggggtgga gctggaggac gacgagaacg
     5401 acgtcgcgct gcgatgggtg gcccgcaagg gcaagaagac gggcatcagc gtcgacgggg
     5461 gggtaagctt ccgaggcggc gaggtcgacc ccttcgtccg cgtcaacacg agctacaaat
     5521 accggatctc cgagaattgg cgcggccagg caaccaatcg cctttactac ctggcccgca
     5581 acggctggcg ggacgacctg cgcatctact tcaacaggcc catcggcgag cgcttcctgt
     5641 tccgctcgcg gtcccgcctg gaatacttcg aggacaagga ctcaaacccc cagctggagc
     5701 agaagttttc actctttcag aacatcagcg accggtcagc catcgcctac gaggcgatat
     5761 ggcgccagga cacggctgag gactcgatct acgacgacga ggagatcctg attgggccaa
     5821 gagacaaata ccagcaagtg aagctgcgca ttcgttaccg ccggaatttc tggcggccct
     5881 ggatgtttgt cgagttctgg ccggtggtcg gctgggtgga ggagcgcgac tgggataccg
     5941 tgctcggtgc ccggatgcgc ctcgagatca acttcggctg gcatgccggc tctaagctcg
     6001 atgagtaaac gcgagcccgg gctcgctgcc tttgcggcgc tgatgctcgg cttcaccgga
     6061 accaacctcg ccggctgctc cccttcggtg accgaggtcg atctgatcat gcccgagctg
     6121 cccgtcgagc agatcatcgc gcgcaagatc gcggacctgg tggacgagga ctccggcctg
     6181 aagatcaatc tgatcccgtt acctgacgac tccctctcgg tcctcgacgc gctggagcag
     6241 ggttacggcg acatcgcgtt cgcgccgaat accaacgagc atcgcgacac catcaccacg
     6301 atcatgccca tctatccgag cgtgctccac gtcggctcga tcgccgatcg cgctgacggc
     6361 agcctggagg aactgctcga cggggctcgg gtatttgccg ggccgccggg ttcgataccc
     6421 cgccagatcg gcgagcggat cgtgagcggg ttgcagctcg aaaagggctt atcgttcacg
     6481 tccgatcagg acgcggcccc cgacgttatc atcctgtttg cgcccatcga ccggaggcgg
     6541 gtcatgagca atccgagcct tgaaagcctc aagctcttct cattcgggga ccccggcgac
     6601 accaggccag gctccatcgt cgatcgaacg gtgctgctga acccgacgct gcggccctat
     6661 gtcatcccgg caggaaccta cggatcgctc acgccggacc ctgtggtgac ctttgccgtc
     6721 gataccctgc tggttgccag ggaaagcctc gacgagacgc tggtgttcga tctgttcgag
     6781 gagtttctcc gcctgcgtcc cgcgctcttc agcaagcgcc cggagctctt tcagcccata
     6841 gacgacgaca ttgcccgcgc ccactggacc ttcagcctgc atcccggcgc gctcgactac
     6901 ctgcagcgcg acgagccttc gctcatcgag cgttactccg gcgtcgccga ggtgctggtc
     6961 acgctgctgg tcggcatggt gagcggagcg ttcgccatcc tcaagatcta tcaggtccgc
     7021 agaaagaacc ggatcgacgc cttctacacg caggtgatcc gaacccgcga ctccgtggca
     7081 gagggcgcgg gcagcgaaga gcgggcagcg gcgctcgccc agctgcgatc gctccgggaa
     7141 cgggcatacg agatgctcgt cgaggagcag ctggccgcgg acaccagctt cctgatcttc
     7201 gtgctgctga ccaacgatgc catcgcccag atcgactccc cggagaacgg gccatccgtc
     7261 tagcgcggca cgcccgcgga gaggacgagc cgggatgagg ccgtccagca ccaaaccgac
     7321 aaaccagcag gagaatcaaa tggcataccc acgtcctgag ctgctagagg aacccgcggc
     7381 actcgccgcg cggctggacg atgccgatct gcggatcgtc gatgccaccg ttttcctcgt
     7441 tcctgccgag cgcggctacc gagcggagag cgggcgcgaa cgctacgagc aggggcacat
     7501 acccggcgcc gcgttcatgg acctcatcga cgcgttctcc gatacgacca cgggcctcgg
     7561 cttcagcctg ccggatccgg gggtcatcgc cgacgcgctc ggccgcctgg gcatctccga
     7621 cgacagcgag gtggtcgtct actcgacggg ccacctcatg tgggctaccc gtgcatggtg
     7681 gctgctgcgc tacgtcggcc atcagcgagc gtccgtcctc aacggcgggt tcgacgcgtg
     7741 gcgcgatctg gggcttccgt tcgagaccgg caccgcgagg cgagcagcgg tgacctaccg
     7801 cccccgacca gacgccagcc tgttcaccga tcgggacggg atgtgccgag cgatggacga
     7861 gcctggtgtc tgcacggtca acgcgctctc accggaggta tacgcgggca ccggaaccac
     7921 gaactacggc cggccaggcc acattccggg cagcgtgaac gtgccctacg aagaactgct
     7981 ggcgggcgga cgcttcaagc cggccggcgc gcttgccgag gcgctcgaat ccaaggggct
     8041 gctcgatgcc gaccgggtcg tgacctactg cggcgggggc atcgccgcca cccttgacgg
     8101 cttcgcctgc ctgctcctcg ggcacgacaa cgtggccgtc tacgacggat ccatgtccga
     8161 gtgggcgcgg gacccctcat tgcccctcaa cgtaggcgag gccccttagg gcacatacgg
     8221 cacatacggc acatagggca cataggacac ttagggcacg ccatgggccg ggcgccgcgt
     8281 gcgtccagct ctcctcagtc ccacaggccc cctagagaga gcagctgcgc atcgagcttc
     8341 acgggctcgc catgatggct cgccagccag cgcgcgccct ccctttcgaa ggcgaccagc
     8401 ccgtccagaa agtccacgtc gacgtcgcgt acgctctggc ccagcaaccg gtcgcggacg
     8461 aggtgaaggg tcatgtcgtg ggacacgcag acgtcgagcc gtcgcgcccc gtccatggcc
     8521 gaaagcttgt cggtcatcag acgaaccacc agcgctgccg caagccgggg cggcatcacc
     8581 gcatcctggg gaacgtcacc ggaaaaccaa gcctccagga agggcaccat tcctccggcg
     8641 gattgcatga cctgccacat tttcatctgg tccagggcat agaagacgcc aagcgcctcg
     8701 accgggcgat gccgagtcac ccgcccgccg gtggacgcgt aaccggccag gacgagctcg
     8761 gctgtttcca tgcatcgctc cggcgggctg gcatacgcgc gcaccgtcag gtcctcgggc
     8821 aaccgctccc cgagccgtcg ggccagatca cgtccttcct cggtgagcgg gttgtccaga
     8881 tcgtgacgcc cgggcgcgaa ctcccgagcc gagtggcgca tcagcacgac ggcgtttttc
     8941 acaccgcccc caaacaggtc gttcaggagc cgaacggtgg catcgccggg cgcggtcaat
     9001 ggactccccc ctgtcaaata gcaccacgta accttctgta ttagtatcga attctcacgc
     9061 cttttaatag agatattcac tgtcccgctc gaacatcggg caccgggcac ccagggggaa
     9121 gcaatggacc taggcatctc tgagcgcgtc aagccgctca tcgacgacat tactcagtac
     9181 attcacgatt acgtggtacc caacgagcgc gcctacgccg agcaggtcga tgcgggcggt
     9241 cgctggaccg aaaccgaggc catggaggag atgaaggaaa aggccaagga gcgcggcctg
     9301 tggaactttt ttcttccgga cagcgaacgc ggttccggac tcaacaacct cgactacgcg
     9361 cacgtcgccg aggtgctggg gcggtacccg ctctcctcgg aggccatgaa ctgcgcggcg
     9421 cccgacaccg gcaacatgga agtgatcgag cggtacggct ccgagcagca gaaagaggaa
     9481 tggttacggc cgctgctgga aggaaagatc cgctcggcgt tcgcgatgac cgagccgcag
     9541 gtcgcttcct cggatgccgc caacatatcc ctggaagcga ggctcgaagg ggacgagtgg
     9601 gtgctcaacg gcgagaagtg gtggacctcg gggatcggcg acccccgctg caagatcatg
     9661 atcgtgatgt gcgttaccga gccggacgcg ccacgacact cgcggcagtc gcaaatcctg
     9721 gtgccggtgg aaaccgacgg cgtcgaggtc ctgaagatga tgcacgtctt cggctacgac
     9781 gacgcgcccc acggacacgg gcacgtccga ttcaccgacg tccgagtacc aaaggacaac
     9841 atcattctcg ggtccggtcg cggtttcgag atcgcccagg gccgactggg tccggggcgc
     9901 atccaccact gcatgcgttc catcggcgtc gccgagcggg ctttggaact gatgtgccgc
     9961 cgcgcgctat ccaaagaagc gttcggcaag cccatcgccg acctaggcgg caactacgac
    10021 aagattgccg atgcgcgcat caacatcgag atggccaggc tgctgacgct gaaggctgcc
    10081 tacatgatgg ataccgtggg caacaagatc gccaggagcg agatcgccca gatcaaggtg
    10141 gcggtaccca acatcgcgct gaaagtcatc gacgacgcca ttcagatcca tggtggcgcc
    10201 ggcgtatccc aggtatttcc gctggccaag atgtgggcag gacagagaac tctacgcctt
    10261 gccgacggcc ccgacgaagt gcaccggcgc gcggtggcca ggctggagct gggcaaatac
    10321 ccggacaaag atccgatccg aacggacgtt tggtaggagg cgatcgtcat gggccgactg
    10381 gatgacaagg tggccgtcat caccggggcg ggtcgcggga tcggccgcga gattgcgctg
    10441 atgatggccg gtgaaggtgc gaaaatcgtc gtcaacgatc tcggcggcaa catggacggg
    10501 accggaaccg gaatgatcgc cgacgacgtg gtggccgaga ttcgcgcggc cggcggcgag
    10561 gccgtctcga acaccgcatc cgtcgccgag gtggcgggcg gcgagagcct tctgcagtcg
    10621 gctctgggcg cgtttggcgc catggacatc ctggtcaaca acgcggggat attgagggac
    10681 cggaccatct tcaagatgga ggaggcggac tgggacgcgg ttcaagccgt gcacctgaaa
    10741 ggccactact gctgcacccg ccccttcgcg cgctacatcc gggaaaacaa ccgcaccggc
    10801 tgcagggtcc tcaatttttc cagcgtgtcc ggtctctacg gcaacttcgg acaggccaac
    10861 tacggagcag ccaaggcagg catcgcaggg ttttcccggg tgctggcact ggaacttgcg
    10921 aagtacggct gcacggtgaa caccatctcg cccggggcgc tgacccgcat gaccatcccg
    10981 ttgcgggagg cgcgcggcga ggaggtgaag gacgaggaga tcagcgagag cgggccgcag
    11041 cacgtcgccc cgatctgcgt gtggctcgca acagaggcgg ccgccggcat cacgtcccag
    11101 atattccaca ccgcgcgcgg cggggtcggc atcatgcagc agccccgggt catcaagcag
    11161 taccacaagt cgagcggcct gtggacgctc gatgagctcg acggcatcgt tcccgagctg
    11221 gtcgccgcgc gggcggccaa cgaggccgcc gccaaggatt ccgggcaacc catcgaggtc
    11281 tgagggaaaa atcccctgag cggctggctc accggccgct cacaactatc ctcgaacccc
    11341 cgacaaagcg gggacaactc gccggcgcgc ctgtgacaac ataggcgctg actctctaaa
    11401 cccagactgt agtgaccgag gagggttttc ccatgggaat tcagcgagca gccatcatga
    11461 tcatcgtcgg cgccctggcg tgggcgacgg caaacggcgc ctacgccggc aagatcgttt
    11521 cctgggtgga cgaaaaaggt gtgacgcact tcacggatgc cggccttgcc aacggccaga
    11581 cgaatgcggt gaccgtggct acctccgaca cgcccgtccg cgccgccccg gaggacatcg
    11641 tccccgagcg gaagagcacg ccaaccgttg cccaatcggg gtccggcaag tcagagggcc
    11701 agaaaagcgg cctgcgcggc taccgcagca gctgggcgcg cgatccggac cgcttcaaga
    11761 atctcaactg aggatcatcc gtttttgtga ggcgccagcg ggtcagaccg tctgcccgcg
    11821 cctcagaaat agtagaccca ccgcgcgtaa aggcgcgacc cgccccctac ggtgagacgg
    11881 gagcccgccg gatccgcgac cacgggcacc ccgccgaact cgtctcctga accgccgagc
    11941 ggttcgacca ctccgagctc gagacgctgg ttgtcgccag ggtcgaagtt gatctgcgtc
    12001 tgcagaagca ggctgccgtc gttgagattg ccaatgagcg tcacggtctg aaccagcaga
    12061 gggtgccact ggtacgtcac tccgccggcc agatagtcgc gcatgacgtt gaacaactcc
    12121 cctcggccga gacggtcgag caggggcgcc gggagttctt cgacggtctc cggcagctct
    12181 gatacgccaa agccgttgtg aaaatactcg gcgaacacgt acgtcgttcg gcccgcgacc
    12241 tccaggctgt agtccagatt gaccaccccg ccgactctga cgccgccgtc cgcgaggcgg
    12301 gtggccacca ggtcggtgcg cagcatggct cctttcaggg gatatctgac gccaatcccg
    12361 tatacctgat cgcggtagtg cttggcggcc atcagctcca cctcgcaggc cccgataaac
    12421 ccgcgccatt tgcccgcgag gctccccgcc tggcccgtga aatcacccgt ctcgtcacgc
    12481 cgtcccaccg cgagcacctg cagatcgctg ccatcggaga agaggcgctc gatcagcagg
    12541 agatcgtcgc ccgccttgta atcccggtcc accgcggtgg gcgcgaacgg gttgaacagg
    12601 tccatgggcc ggaataccag gccggcgccc cagctgacgg cctcgcggcc aacggtgacg
    12661 gcccagtcat ccctgcgcag cttgagggcc agccggtcga ggcgatggaa cgagcggtgc
    12721 cggtcgccgt cttcgatctc ccaggtcagg tccatcagcc ggcgctcgtc tccctcgggg
    12781 gactgttcga gaaccgtgcc gggtgccgtg gggaagttca gcgaatctcc cgtgaccagc
    12841 gtggtcgagt gctccacctg cagcgttacc gccgagatcg tcttctgaaa catcaggcgc
    12901 aggtcgtaat tctggtcgta ggcgggggtg ccgctgaggt tgcgctgaag atcgtgctcc
    12961 ggcagggcgg tagccgtgcc gaaccatttg acccgagcgg aaaagtccga ctgcgccgcg
    13021 ctgcccgtcg cccacagacc gagaaacggc ccgatcagcc agcttctatg ccggcgccac
    13081 atcggcgctg tccttcacga tcgcgccgtc gcgcagcccg acctgacgct gagcgtagcc
    13141 catgaccatg ggatcgtggg tggcggcaac gatggtcacg ccgcggtcct ggttgaggcg
    13201 acgcagatac tcgagcagct ccctggtggt ggccgaatcg agattggcgc tcggctcgtc
    13261 ggccaacagc agaaccggct cagtgacgat ggccctcgca accgccaccc gctgctgctg
    13321 acctccggac agctcccccg gacgtcgctt ctccagctcg ccgatgccca gggcctcgag
    13381 cgcggcgcga gcccgagcgg cacgctccgc ggccgacacg ccctgcagct gcatgatgaa
    13441 ctccacgttc tcccttgcgg acagaacggg aatgaggtta taggactgga aaacgaagcc
    13501 gatcttgcgc agccgcagat ccgacagccg actctcgctg agcgaaccga ggtcaacgcc
    13561 atccacctcg acgcatccgg agctggggcg gtccagcccg ccgatcatgt tgagcaacgt
    13621 ggactttccc gatccggacg gtcccgccag cgtcacgaac tcgcccttgc cgacctcgaa
    13681 gtccacatcc cgaagagcat gcacgggcac cgaatcgtcc tggtaggtcc gggagacccc
    13741 aatacagcgc actacccgtt cggccatttc ttcatcatct ccgcaaagca tccagggggc
    13801 cgatctgcac ggcacgccat gccgggtaga gactcgcaat caggccaaag acaatgctca
    13861 tggccgcgac cagcacgacg tcctcgagga gaagtctcgg aacgagcacg ctgcgcatac
    13921 cgaagagctc cacgccggcc gcccattccg tcaggtcgat tccgtcgctc agccacacga
    13981 ccagcagaaa acccaagccg agccccaagg ccaccccagc ggccatgatg agggtggatt
    14041 ccagcacgac ctgcaccacc accgagccgg gacgcatgcc gacggctcgc agcatgccga
    14101 actctctgat ccgctccatc acggcggtga tcagggcgtt gacgagcccg aatatcaaag
    14161 cgcccatcat gatgcagaac cagatgaaga tcgccgtatc cgcgaacagg aacatggccg
    14221 ccgtctgcgg ttcgagttcc ttccagtcca tcacgtccag gtcggtaaag gtttccgcca
    14281 gcccctgctt gaggccatcg cctcgaggct cttcgcgcag cttgatcgac acctcggtga
    14341 agacgttggc gtccacgagc tcctgaaggc gcgtcacgcc ggtgaagacg aacatcttct
    14401 ccagcccctg accatcggcg tcgtaaacgc ccgcaatccg gaatccggct tcgcgattga
    14461 gcccgtccgc gccctgtgtg atcagcacca ggcgcctgcc cacgtcggta tcgagctgct
    14521 cggccagcgc ctgcccgatc acgacccgcc gatcggctgc accggatagc ggctcgccgc
    14581 ggtagctggc atcccccaga aaggagatcg tctcctgcgc cgggtccacg ccgacgaact
    14641 gcacgccgcg ggtttcccgt tcgctcatga tgacggccgg gatcctgatg cgtgccgccc
    14701 atccctgaag cagccggggg ccgatgtcgg gcgaccagcc ctgcaccaga gtgaagcttc
    14761 tctgaattcc cgggtcgtcc agatagccgg gcgcaagcac tttcagatga ccggtgaggt
    14821 tctgcacagc ggcttctctc atgtcgtgct gaaaaccgcg aatcagcgcg ttggccaggg
    14881 tcacgccgcc aaccgcaacc actaccgcca tgagcagcat caggttccgg cgcttgtgcc
    14941 gaagcaggtt tcgagcagag agctgaaaga gaagcttcaa catggacgac accggcgcgg
    15001 cgacccgaac accctccctg tcctccgggc agccctcgca ccgcccgcgt tcgaagcagc
    15061 gtcacccgac atcattcgcc tctcagcgct gcggcggcgt tcagccggaa aatgcgcaga
    15121 gacggaagga gcgcggcgat ctgggttccc atcagcatga ccagcggcgc ggagagaaac
    15181 gcctgaagcg aaaacgccgg gtaaagccgg gccggcatgt agaactgctc ggcatactcc
    15241 tcgagcgtct cgcccatgta tatgcccacg tcctgaagcc atgccacgat gatggcggcg
    15301 aggcccagcc ccagcgaagc cccgagcagc cagatgaaga acccctccca ctgcatcatc
    15361 gcgacgatgc gccagggctt catgccgatg gctttgagca tcccgaattc cctggtccgc
    15421 tcgaagacgg tcatgatgaa ggtgttcacg atgctgaaca cgacgagcac ctcgacgacg
    15481 tagaagaaga gctcaccccc gagccggtct atctcgatgg cttgccgcag ctccggcatt
    15541 acctcgctcc aatgcctgac cacgagcgcg ggccggtcgg tcaaactgtg cctcagactc
    15601 tcggcgacgg cctcgctgcg atccagatcc tccgtgcgga tggcgaaagt gtgcacctcg
    15661 tcggcaagcc cgaatgcgct ctggactgac gcgagcgggg tgatgagggt gacccgatcc
    15721 agatcggcta ccccggtctc caggatgccc gccacggtga tcaccatggc ggcgacaccg
    15781 ccgtgcttct cagatcccaa gagcaccacc tcatcgccga gagtcacgcc gaggttgcgc
    15841 gccagcaaag agcccagcag gccgtcatcc ggggcggcaa tggctctacc ctcgcgaacc
    15901 atcttcagca ggcgcacggt atcgcgctcg gcgtcgggat ctacgccgat gatccgggca
    15961 ccaaatgacc gctctcccgc ggaagccagc gcgaacgctt ccgcgcgcgg cgcgacggcc
    16021 tcaacgccgg cggtggcttc gatcacccgc cggagcgcac cggcgctccc catggtgtcc
    16081 tcgaagcggg cgctgtcgac gtagtcggcc gtttgaatct ggatgtgacc cgtcagcagg
    16141 gaggtggcgt tctccatcat gatgtcgtac tgaccgagct gcatcgccat ggagaaaacc
    16201 accagcagca ccgcaaacgc gatgccaccc gccgtcagcc acgtccgcct gcggttgcgc
    16261 gccaggttgc gccaaccgat tcggagcagc agcgccgggc gggcacgccc cccggccgcc
    16321 ggcgtccgcg cctgggtcac ggccgctggc ccgactgcag cgagaagagc gtgaagattc
    16381 ggtcttccag ctcgatgtcg aagtcgaccc gggcgtagct gagctcggtg tagttttccg
    16441 gctcatcgag cttggacatg cgcattcggg taccgaagat ccgcccgccc atctcgccga
    16501 tctccagggt ttccatccgc ttgagcggac gcatggcctg atcgaagaac gtctgcgaga
    16561 tcagaacgtg gtcgtcgcgc agcacgatct cctctttgcc ccacaccacc ggcgcctctt
    16621 cgcggggaac cgcctcgatg gtgtaaaggg tgtggccctc ggatcgccgg gtcgccgtga
    16681 tggccagctc gtattcttcc aacagcttgt ccgtgcggga gagatcgttg tatgagaagt
    16741 ccgagcctgc ccagctctgc gacatcaggc tgaagggcaa ccggatcgtg cggttcagct
    16801 tcgggttgta ggtccacatc ttctcgccct gcttgagggt cgcgttgccc gcatccctgg
    16861 ccggcgccgt gaaccggatc agggcgtcct tgcggccgcg ggtccaggcg accagcttcg
    16921 aggtccgttg ccaatccggc cggtgaatga ccatggcgaa ctccgcgtaa gacgattgcc
    16981 cccgggtgag atcgatggcg gcggcgacca gctcccgagc agtaggctct tcggcggcga
    17041 gaacgccggc cgggaagatg acgagcatcg agtgcgcagc ggtgagcagc agccgcttga
    17101 cgctatgcat tgacaagcct ccacgagcag gcaatatccg gatcggcctc aacgtttgac
    17161 tctatcaaca attattgtcc ccatgccgct gagagaagag aagaaacgac aggtccggaa
    17221 ggacatacta gacgctgcgc gagagctgat cgagcaacgc ggatacgaca gctcgacgat
    17281 gcgcgacatt gccggaaccg cacgggtgag ttaccagacc ctgtacaact attttccaac
    17341 caaggcggcc atcctcgccg ccctgctcaa cgacgtcgtg gagcaggcgg tcgccgacat
    17401 cgaagcgctc atcgagggct acgatggcag gctggtggaa accctgaacg ccatcaaccg
    17461 ggcccggatc gatctcgtcg cgcgacggga cagggagctg tggcgcatca tctcggtgga
    17521 caggctgcgc gacggccagg acgcgggcgc cgtccaccag cggcttgacg acgccgccgg
    17581 ggaaaggctc aagcagctgc tcgccctggc gaggcaaagg ggcgagctgg tcccggacgt
    17641 ggacgtgggt ttgctcagcg acactctcaa ggccctgggc gagcgggcgg tggcgcgcta
    17701 cctggaggct tccgccgctt cccgacgcag cgtcgcggag tccctggctg cccagacctc
    17761 gctgctggtc acgccctacc tggagccggc gcgatgacca tccgctgggg tattctcgcg
    17821 acgggcagca tcgctcacac gttcgcccgg gccgtaaagc tgtcggcgac gggcaggctt
    17881 caggccgtcg gcagccgccg gggagagtcc gccgaggcct tcgcgcgggc cctcgacgtg
    17941 ccgagcgccc acgggaccta cgccgagctg gtgaacgacc ccgaagtcga cgccgtctac
    18001 atcgccgccc cgcaccccga ccacgccgag tggtcgatac gcgcgcttcg cgcgggcaag
    18061 gccgtgctct gcgagaagcc ggcagggctc aaccacgccg aggtgatggc cgtcatcgat
    18121 gaggccgagc agcgccaggc attcttcatg gaagcgttca tgtaccgatg ccatccgcaa
    18181 acgcttcgcc ttgtggagct ggtgcgggaa ggggccatcg gcgaggttcg ccacatcgag
    18241 gcccgcttcg gctttcacgc cgacttcgac ccggcgagcc gcctcttcgg caacgccctc
    18301 ggcggcggcg gcatcatgga cgtcggctgc tatccggtct ccatggcgag gctgatcgcg
    18361 ggtgccgagc ccgaccaggt cagcggccac gggaaggtgg gtgccagcgg cgtcgacgag
    18421 tgggcggccg ccttgctgaa gttccccaac gggctctccg cccaggttgc ctcgtccgtt
    18481 tccctggctc tggacaactc cgttcgcctc aacggcagcg cggggcagat catcgtgaag
    18541 aatccctggc actgcgccga tgcggagggt cgatgggcgt tgcagattcg ccggaggggg
    18601 cgtgatccgg agacggtcga aggcaccgcg ccccctctgt acgagctcga ggccgaccac
    18661 gtcgccgaca tgatcgctca agggcggagc gaatcccccc atatgagctg gaccgacagt
    18721 cgcaacaacg cgctggtgct cgacgcctgg cggcgggagg tgggcgtcga gtttcagcgg
    18781 gaaaccaagg ccgcgccgct gctgggcggg cccctgcgca ggccggagag accgccgatg
    18841 acctacggcc gggtcgccaa cctgtccaag ccggtagccc gcctggtaat gggctgcgac
    18901 aaccagccga gcctgagcca cgctgccatc atgtgggacc actacttcga gcgtggcggc
    18961 aacgccttcg acactgccta catctatggc ggcggcagca tggaaaagct gcttggtcgc
    19021 tggcacacca gccgcagggt tcgcgacgac atcgtcatca tcggcaaggg tgcgcacacg
    19081 cccgacaacc gtcctgaagc cgtctcccgg cagctggacc ggagcctgga ccgactccag
    19141 accggcgagg tagacgtcta cttcctgcac cgcgacaacg tcgacatacc ggtaggggag
    19201 ttcgtcagcg ccctgaacga cgaagcggcc aagggccgca tcaaggcgtt cggcggatcg
    19261 aactggaccc tggcgcgggt aaaggccgcg aacgcctacg cggcgcagca ggggctgcag
    19321 ccgttcacgg tgctttccaa ccacttcagc ctggcccgga tggtggagcc catctggccg
    19381 ggcgtcgaag ccgcttccga tccggagttc agggctttcc tggccgcatc gaagattgcc
    19441 ctgtttccgt ggtcgtccca ggctcgtggc ttcttcacgc cgtgggcgga tcccatcatc
    19501 gccgaccggg gagcggagaa tcgcgcggtg accacaatgg aaccgaccat cgccgagctc
    19561 aaacgcacgt ggttcgccca agacaacttc gagcgccgcg cgcgcgctgg cgccgtcgcc
    19621 gagcggctgg acgttgagat gatcaacgtt gcgctggcct atgtcctgaa ccagccgttt
    19681 ccctgcttcc cgctcatcgg cccgagattg ccgtgggaga cccgcagcag cctcgcagcg
    19741 cttgaagtgg cgctgagcga cgcggagagg gcctggctgg atctcagcgc cgacggcggc
    19801 tgacaagcga aaccacgttc tctccgccga tatggtcctg ccgcagcgag agggcgatct
    19861 cccaggtgta agtggtcttg ccgtcgcgga gaaacccccg gaagccgagc ccgccggcaa
    19921 tctcgaaaag agtaaacgtc gcgtgaacgg tctgctcctc gttccagagg agccccaggt
    19981 tcggggggct gtccagcttg gccgttccgg tcagagaacc cttgagggtg cgcacgctca
    20041 ggtcatacgt cccctgttcg tccggggcga tcctgacgtt catggcccac gagcccagct
    20101 ccttggcatc cacttcggcg ctgatgcttg caccctcgtc gtcccgctgc acctccacat
    20161 cacaggcaac cccctgcccc agactctgcc cctcggcgag gattgagccc cggcccgtcc
    20221 actggcccga tcgaatgagt atcatgcggt gacgtcctcc ctgatggcca cggccgtgcc
    20281 gatggcttcg gtgcagcggg aatctttgtt gcgggcattg ttgacgtagc tggacaccgg
    20341 caccgcgtcc agcgcgacgg gaacgcgcgg agcacagagc gctttgaggg cggaggcctg
    20401 cgtcgaagga tcgagccaga tccgggcgtc tttcataccg agcataacgg gctggcgttt
    20461 atgaacgaac gtcagctgct cgttcgcctc gcaggtgagc accgcgaagc tcaacagcgg
    20521 tccgttttca ccgtcctgcc agctgtccca tattccggcc agcagcatgc cggaagccag
    20581 atgaggcgtc aggtaatacg gcagcttgcg ctccttgctg cgagcccact cgtagaaccc
    20641 gctgaccggc accacgcatc gacgccggct gaagggttcc cggaaagccg ggcttttctc
    20701 cacggtctcc gacttggcgt tgaacatgct gtacttcgtc gtcaccgact tggaccagaa
    20761 gggggtgagc caccagcgca tcgcaacgag ctccggccgc ccgtcgtcgt tcatacgcag
    20821 aacgacgatg gtctccgtcg gcgcggcatt gtaattgtct ggtcccggat gcggcagccc
    20881 gaccagctcg agcagcagct ccgaaagcgg gctggaggta acgttgaaac gtccgcacac
    20941 ggcgcgcgcc ctcagccgtg cttgccgatg gcccaggcga gctcgcccag tgacgccgcc
    21001 gcgtagtcca ccgcgggcgc ctcgagctgg gtcgccatgg tgtcgttcca gcggttgagg
    21061 aacccgaaga tggcaatcac cgccacgatc tgcacgatct gccgctcgct gaaatacgcc
    21121 cgcagcctct cgaagtgcat agcatcggcc tgattgggca cggcaccggc agcaagcgcc
    21181 agaccgatcg ccgcgcgctc ggcgtccgag aagacggggc tgttctcgta gtcgagaacg
    21241 gaatcgaact tttcctgctc ggcgccctga cggtgtccgc tgtgggcggt gtgggcctga
    21301 caataacggc agcccgagct gacgctggtt gcgaaggcaa tgagctgaat cagaggcggc
    21361 gacaggttct cgccggcatc cccctgctcg gggatcagcg gctggagctg atcggccatg
    21421 gccttgagat cacccccgaa gatcacgttc gtcaacatca tgaaacccat cggcagctgc
    21481 ggcatgtggg ccatggtcag catgctgttg ggcacgaacc ccatggcgct ctcggcagca
    21541 tcgaatatcg gcttcagcgc cgggacgtcg tctatcgatc tgggctgcac gtgcgccatg
    21601 ggcttgcgct ccaatgagtt gggttgcttg agtttactgg tttgcgtttt ggcttacatt
    21661 cgccacactg ccgagccaac gaacgctcga ggctcgaatg atccgaccac ccgtcgccaa
    21721 acgccgcaac caccgccaca cccatcacgg catcaccttg gaggatgcgt attactggct
    21781 gagggatccc gcctaccccg acgtcaagga tcccgagatc atcgaatatc tgacggcaga
    21841 gaacgcgtac ttcgatgctc ggatggaggc ccaccagccc ctggtgacgc aactgttcga
    21901 ggagatcaag ggtcgtcagc ccgcggagga cgcctccgtg ccccatcgac acggcgcctt
    21961 ctggtatcag tggcgctatc agaaggatgc ccagtaccga cagtggctgc gcgcaccggc
    22021 acccgcgggt tcctctcctg atgccggacc atgggaagtg attctcgacg agccggagct
    22081 cgcccgggaa cacccgtact tcagcctggg cggaatctcc gtgagccgca gcggccgctt
    22141 catcgcgtgg agcgccgaca cgagcggagc cgaacgcttc actctcagga tcagggatct
    22201 gcaaacaggc gagctcgtcg acgatcccat agagcaaacc cagggaagcc cggtatgggc
    22261 ggccgacgac acgtgctttt tctacgtggt cgtcaacgat caatggcgcc cgtatcaggt
    22321 gcgccgccac aagctcggcg acccgcccga tgctgacgcc gtcgtgtacg aggagcccga
    22381 agactcgttc ttcgtttccg tggacgagac ccagtccgaa gcctatatcc tcattgccgc
    22441 cggagatcac gtgacctcgg aaatcaggta cgttccggct gacgagcctc ttgcagagcc
    22501 ggctctgatc gcgcggagaa gggcgggcca cgagtacgaa gtggatcatc gcgaaggcgc
    22561 cttctacgtc gtcaccaacg acgcgcacaa gaacttccgc ctcgcccgag ccccggaaga
    22621 cgatcctgtc gagcagaact ggcagacggt catcgaaggc tccgagagcc actacctcac
    22681 cggcctgctc tgcttcaaag atcacctggt ggtgtgcgag cggatcgatg gcctggacca
    22741 gatacgtatc cgcagcggcg aagaggaaca ccacgtcgtg tttccggaga gcgcatacgc
    22801 ggtcggcctg ggaacgaatg cggaatacga cagcgcaact ctgcggctgc actacgaatc
    22861 catggtcacg cccagcacgg tctacgatta cgacattgca cggcgcaagc tgattcagcg
    22921 caaagtccgg gaaatcccaa ccggctacga ccggacacga tacgaaaccg cgcggcgcac
    22981 cgtaaccgct cgcgacggcg cgcaggtacc cttgagcatc gtgtacccca gagactttcc
    23041 gcgcgacgga accgcgcccg tgcacctcta ctcctacgga gcgtacggcc atgccatgag
    23101 cccgacattt tcgccgagcc gtctgtccct tctggatcgg ggcttcgctt tcgccgtcgc
    23161 gcacgttcgc gggggcgacg acctgggacg acagtggtat gaagacggca agcttgacaa
    23221 gcgaaccaac acgttcaacg acttcgtgga ttgcgctcgg cacctgatcg actccggatt
    23281 ctcttcaccg gggctggtcg tcatatccgg cggcagcgcc ggaggcgagc tgatgggtgc
    23341 tgccatcaac gcagcgccaa cgctgtgggg agccgtggtc gctcacatgc ccttcgtcga
    23401 cgtgctgagc acgatgctgg atgccggcct gccgctcacc cccatcgagt ggccggagtg
    23461 gggtaacccg atcgaggaca aagcggcctt cgagctgatc agatcatact caccctacga
    23521 ccagctcgcg cccggcgcgt acccgcccat cctggttacc gcaggactca acgaccccag
    23581 agtcacctac tgggaggccg caaagtacgt cgccaagctg cggcatctca agacagacga
    23641 gaactggctc ctgctgaaaa ccaacatgtc tgccggccat agcggagagt ccggccggtt
    23701 cgactcgctt cgcgaactgg ccgaggaata tgctttcttt ctggtgagcc tcgaagccgc
    23761 cggatggcat ccgcgcggcg aggacgatca ggcgtgatga accaggtgac ggacctttgc
    23821 ctcgttggcc tggcgcatgc gcttcttgat gatgttctgc acaacggtga ccgtcgtcca
    23881 gtcgtgcatc atcagacgcc cgcacccgtc gcactcggcg cgctcgcatt ctagaacaag
    23941 gtccagaaga ccgctgtagg acaaacgtga gatatgcttg cagtggccgc actccaggcc
    24001 caccgcggga tcgatgccta cgtaatccat gacgctgtac tccctttcgt cacgcccgcc
    24061 attctaaagg acggatttcg tgattgagcg gtactttagc ggctttgtga ctccgtgcac
    24121 actcctgcta tgacgcagtg gcgcctgacg cgccagacca agccttgcag tcactgccga
    24181 acgacgggta tcagccggag gccactttcc gcaggccatc acggaattcc ggtgccgcga
    24241 tctcaatgag cgcgcgtacg cgctcggccg ccgacagcgc acggagctgg gcgacgccat
    24301 gctccgtgac aattatgtcc acgtccgtgc gcaacgcggt aaccaactcg actttcggca
    24361 cgatgcggga aaccttgccc ccccgagcgg ttgcgttcat ggcaacgatg gatcgtcccc
    24421 ccggagaggc tttcgccgcg cgcatgaagt cgacggagcc gcccgttccg gaaatctgcc
    24481 tgccgccagc gacctcggca ttgacctggc cgaacaggtc cacctcgacg gcggagttga
    24541 cagaaagaaa ctgcggcagc tcgcgtatca cgttgacctc gtgagtgtgg ttggctcccc
    24601 ggaactgtac cgccggggtt tgagccagcc agtcgagcag ctggcgagat ccgagcgcca
    24661 tgccggctat gtgttttcct gtgtctatgg gcttgttaac gccggtcatg ttcccattac
    24721 ggatgagcgc catcccgccg tcgtcgataa gcccgccgtg catgccgaga tcattcctgt
    24781 cgccgagcct tgcaaggata gcggcaggga tgctgcctat gccggtctga atgcagtcgc
    24841 cgtcgcgaac gagttgcgcg acgtgctcac cgatgcgcgc cgccgcccca tctatctccg
    24901 gaggagccat ctcaaacagc gaacggtccg tctccatgag aaagtcgatc cgaccggcgt
    24961 cgaccaccgg acaacccgcg ggggcgacga agccgtgatt cagctcggcc gccacggtct
    25021 tcgcgcccat catcgcggcc tccacgaagt ccacgttggg gcctacgcag agctggccgc
    25081 tggcattgcg cgcaacctgg aggagcacga cgtcgacacc ttcggccagg aagtcgtaca
    25141 cggctcgcat ctgcatcgga aggaaacgca cgcgcgccgg atcggcatcg cgaagatgcg
    25201 gcgtcatgaa aaaggttgtg aagatcgcgt cctcggagaa ggccgtgaag tcgaagcggt
    25261 tgtatccagc cagcggaaac tggaggaact cgatttccgg cggcagagtc tgatccgcca
    25321 gcacctcgag caacccggtc ggctcgttgg aactgcccgc cacgaatatc ctctgacccg
    25381 gctccagcag agccgataac tctttcatga tttcaccatc ctggaggcaa gggcgagatt
    25441 ctagcctcgc ccaggggcaa ttcgtaataa tcggcagatg attcacggca accccgcaga
    25501 cctggtcgat ttgctttcag tccacccgac gagagaggaa ggctatcaag cgggtcaggt
    25561 gcgcgaagca cgctcgcggg tatacggagg ccagtttctc ggtcagggca tctgtgccgc
    25621 cagccagatc gggcacggcg ccctgaggtc ctttcacggc tactatctgc gtcccggggc
    25681 gacggacctg ggcatcggct accaggtgga gcgcctcggc gacatggttg tcggcgtgtc
    25741 cgccgtgcag cgcgagaagc agctgtttct catgatggcg cgcttcggcc ctttctgccc
    25801 cgctccaccc gaggagatgc ccgacgtgcc gccccccgag gactgcattc cacgggagcg
    25861 cggcattgcc ggcctggacc gagacaccga ttccacatgg gccgtaacag actccccttt
    25921 cgacaaccgg ttcgtcgagg acatctggaa cgaagactac cggcgaccgc ttcaccacgt
    25981 ctggttccgc ctgcgcgatc ccggcgcgct gcccggccat atcgatcaga tcgtgatgca
    26041 ggcgattgcc gcctactacg cggacgacac catcatggac aacgcgctct ttccacacgg
    26101 ctggcaccga tgctggagcg aactccagac ggcgagcctc gaccacgcca tgtggttcca
    26161 ccgacccatg gatctcagac gctggcacct ctttgcccag gacagcccgg tggcaagcgg
    26221 cgggcgcgcc atgacccgtg ggaccatatt cgacgatcgg ggcgcggtgg tggcctcagc
    26281 cgcccaggag atccttctgc gcgaaccacc ggacgacaag ccgtgacacc tctggcgcag
    26341 ttcgaacccg gagtacgtgc gtggttcacc gagcgctttc cttcccccac tgccgttcag
    26401 gcgcagagct ggccgcggat cgcagcaggg gagcatgtgc tgatcacggc accgacgggc
    26461 agcggcaaaa cgctgaccgc ttttctgtgg gcgttgaacc agtacgcgtc cggatcgtgg
    26521 gtgccgggcc atacccgggt tgtctacatc tcgccgctga aagcgctgaa caacgacatc
    26581 cggcgcaacc tgctggagcc cctgcgatcg cttcgggagg atcacgaatt tcccccggta
    26641 agcgtgcaga cccgatccgg cgacacgccg caaagcgaaa gacaacgaat gctgcggcgg
    26701 ccgccggaca ttctgatcac gacgcctgaa agcctgtccc tgatgctgac caccactcgg
    26761 ggacgactgg cgcttggcag cgtggagacg gtgatactcg acgagataca ttccatcgtg
    26821 gacaaccggc gcggcgcgca gctgatgacc tcgctggaaa gactgacgga tctgtgcggc
    26881 gagattcagc ggctcgcgct gtctgctacc gtccgcccac tcgaagggat cgcggagtac
    26941 gtgggcggct acacggccga cggcgctccg cgcagggtca gcatcgtcga tccaggctcc
    27001 gccaaggaaa tcgagttccg cgtgcgctac cccgacgaag tcaggctcgc cctggagaac
    27061 ggcaggaaga tctgggatcc gttgtcagag gtttttcggc gccagatcga gcacaacgcc
    27121 tcgactctgt ttttcaccaa cagccggcgc ctggccgaaa agatcacctt gaagataaac
    27181 gaggaccagg ccgggcccat cgcctacgct caccacggct cgctggccag agagatccgc
    27241 acggaggtgg aagagcggct gaaggccggc agcctcaagg ccatcgtcgc gaccagctct
    27301 ctggagatgg gtatcgacat cggccatctc gacgaggtcg tcctggtcca gtctccgcct
    27361 tccatcgcgg ccacgcttca gcgcatcggt cgggccggtc acggcgtggg agagaccagc
    27421 gtcggcacgc tctaccctac ccacgcaaac gactttctcg aggccatcgt cctggccaag
    27481 gcggtagccg ggggcgacat cgagccgctg actcctcagg agaacgcgct ggacgtgctc
    27541 gcccagatca tcgtgtccat gtgcgccagc gaaagctggc agctcgacga tgtcttcgct
    27601 ctgcttcgcc gcagcacccc gtaccatgcg ctgccgcggg agcagttcga tctgctgatc
    27661 gacatgctgg ccgggcgcta tgcaggctca cgggtgcggg agctgaaacc gcgcatcgtt
    27721 ctggaccgga taaacaacac gattcgagcg cataagggcg cggtcctcgc cctgtacaac
    27781 tccggcggca ccatccccga ccgcggctat taccagcttc gccacgccga caccggtgcg
    27841 gtgatcggcg agctcgacga ggagttcgtc tgggaggcca ccacgggtca gatcttcagc
    27901 atgggcaccc agaactggca ggtcacgcgg gtgacgcaca acgatgtgct cgttcgctcg
    27961 gcaccaccgg gcacgacggc tccgccgttc tggcgctcgg agtcgtacaa ccggagcttc
    28021 cacttctcgg agcgcatcgc ccgcgagctc gagcaagccg aagccttgct cgacgacggg
    28081 cgcaacgacg atcttcgatc cacgttcacc agcgcgggcg gcttcgagcc cgaggcggcc
    28141 gtcgagctgc tcgattacct gacccggcag cgggaggcga gcggagcccc cctgccccat
    28201 cggcatcatc tgctgctgga actcgtccgc agcggaccgg gcggttatcg cggaccggac
    28261 gatacccgcc agctggtgct gcatacgttc tggggcggcc ggctgaaccg gccgtgggcg
    28321 ctggcattgg aggccgcctg gcaggacgcg ctcggcaggc gcgcggaaat tcacgccgac
    28381 aataacgcca tcgtcattca ggtaaagggc gaacccgatc ccgcgcgggt gctcgccatg
    28441 gtgacggccg acaacctcga cgagctgctg cgcaggtccc tggagcggtc cggattcttc
    28501 ggcgcgcgtt ttcgag
//
LOCUS       DBNHLM010000243         7195 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1411540, whole genome shotgun sequence.
ACCESSION   DBNHLM010000243 DBNHLM010000000
VERSION     DBNHLM010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7195
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1411540"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="AC16QF_11255"
     CDS             complement(<1..447)
                     /locus_tag="AC16QF_11255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449178.1"
                     /translation="MSKENELAEIRNAVRQLCAQYGEDYWLELDRINGYPTEFVKELT
                     TSGFLTVLIPEEYGGAGLGVLEAAAVMEEVCRSGAHAGACHAQMYVMGSVLRHGNDEQ
                     KRKYLPKIASGELRLQSFGVTEPTTGTDTTSLKTTARLDGDSYVVNG"
     gene            743..1012
                     /locus_tag="AC16QF_11260"
     CDS             743..1012
                     /locus_tag="AC16QF_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449179.1"
                     /translation="MAVVLAMGCAANAIADEEAVDSLQFEETFGFDISQYQLDDYTEV
                     TGWRLSDRWYFGRQKGVDSGLGFVWQDEADQMSISTNGIRFTRRF"
     gene            1119..2321
                     /locus_tag="AC16QF_11265"
     CDS             1119..2321
                     /locus_tag="AC16QF_11265"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6449180.1"
                     /translation="MPVTIPDPDPNNAWRLETPGSQGWTRTARATDPDRYFVVSADGH
                     VQEPSDLWRTRMDAKYRDRLPGVAMDAKGQKFQKTEGFRPLRLQNIKFEGEDALRNQS
                     GHTPEERLADLAADGVDCEILFPNKGLTMWATPDAAFSQAMCRVFNDWAWEVFGPYND
                     RLAPMACVASADIPGAVEEIQRCARLGFRGVTLPCKPVWGAPDHEALNYNLPDFDPLW
                     ACVQDAGLPVTFHVSTGRDPRTARGNGGAVINYAVHSLAPTMEPIANICASGVIDRFP
                     KLRFGTIEAGIGWVPWTLAALDEAYKKHHMWVRPKLKMLPSDYFRQNGFASFQEDQPG
                     LDLAREHRLIDNFLWANDYPHHEGTWPHSAEAIERTMGHLTDSERAKILGLNAASIFG
                     FEVPRGKA"
     gene            complement(2325..2723)
                     /locus_tag="AC16QF_11270"
     CDS             complement(2325..2723)
                     /locus_tag="AC16QF_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6449181.1"
                     /translation="MHPNEALFANESFYLAFAAGDSDAMEQLWSRQHPLVCIHPGWPA
                     LVGRESVIDSWRQILANPDQPEVEFYGARAVELGSGIAVVCYEVLPGTVLVAMNLFIA
                     ENGEARLVHHQSGPCGDPPPRDAVVRRLDA"
     gene            2842..4695
                     /locus_tag="AC16QF_11275"
     CDS             2842..4695
                     /locus_tag="AC16QF_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6449182.1"
                     /translation="MSTLHKPTCLIALFLSVTLMTPPGWTAAREIGPNHWAGVSRVVA
                     FGDVHGAHDQMVTALESAGVVDAELNWAAGDTHLVSLGDLLDRGAHSRQTMDLLMRLQ
                     RQAKGAGGRVHVVLGNHELMNLTGDMRYVAADEFAAFADDEDPSVREAAREAFVAAAD
                     PASGKDPSVEFDERYPAGYFGHRAGFAAAGSYGDWLLQLPLMVVVNETAFVHGGLPAL
                     IADQTLEEINRQYQRDLGEIMALGGRLADAGWVSAERDLLLQDDAVLARLEAAAEGDD
                     GDLSRAGTRFIELVRGPLFGSRGPNWYRGTAMCHASLEETVLKAALKALGAKRVVVGH
                     TPTSNRRVQQRMDGRAILADTGMLRSYYSGQPAPVVLTGDDIEILYPLSETRADAPDV
                     IDPLARYGLDQPRLEAVMSNAAIAEVSTAGTGQRFTTVTLLDEGKRYEFAFERGQGSD
                     NDRKVGAYRLSRLLELNVVPVTVSRTVEGAKGVLIALPEKRVSEIERANQGLSRSNWC
                     SFGNDYQLMYAFDALIRNEQRTTDTMIYDQEDWQLTLIGHEASFGNGTDLPAYLAAVE
                     KILPAAMAARMAAMNEDSLTAALGDQLSKRQIRALLKRRDRMIKDWQLQAL"
     gene            4695..5339
                     /locus_tag="AC16QF_11280"
     CDS             4695..5339
                     /locus_tag="AC16QF_11280"
                     /inference="COORDINATES: protein motif:HMM:NF027640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HIR6449183.1"
                     /translation="MRKSTRASAPVFIITVYYVVLLGGFMAASSYLPGLRVYLPVDGI
                     SELIERGGASFEPVEIIRTAIVEAPHMARLALGMIGATLLMLPISWVYFITTPKSAVD
                     RSFAQTMIMLPIIVAGIAMIVQNSIALAFSLAGVVAAVRFRFTLTQPSYTLYIFAAIT
                     IGLGGGIAAIEISTVVSIAFVYATMLMWMSDYGADMNTSFFSFLTGRDRREEDP"
     gene            5323..6183
                     /locus_tag="AC16QF_11285"
     CDS             5323..6183
                     /locus_tag="AC16QF_11285"
                     /inference="COORDINATES: protein motif:HMM:NF018659.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HIR6449184.1"
                     /translation="MKKILRAFASLAACIAFSFLPGCGSHDETFAFGGYRFATDTFQQ
                     WKLPKSLREISGLAATADGRLFTHGDERAEIYQIDYRDGKLVKSFRLGISSDSRPVKG
                     DFEGIAVRDSELYLITSNGILYRSQIGGDGSTMPYQTFNSGAGDLCEVEGLAYRPQTD
                     MLLIACKEVRKVEFEDKVVVLQWSPQTRRLERDMSILINQSSTGGRFSASGISVSPRN
                     GNLLLVSARQRRLLEVTPEAHVVAVLSLPMERYHRQTEGIEVTGSGTLVLADEGGNKR
                     GRLGTYRGRN"
     gene            6338..7123
                     /locus_tag="AC16QF_11290"
     CDS             6338..7123
                     /locus_tag="AC16QF_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449185.1"
                     /translation="MVYYGDLTNAIRAGRGKAYDEALDLADLTNALDFLKALHTAKKF
                     RRARYEALPGRSSLEEFLADIGAPILSTLRLTDKVIGRRMPEVVAYWDDRDGFSSQIC
                     ARLLNEIRKAKDEDSDILVISHCLGTIAAYDAFWELSRGQDADPSAKIDTWITMGSPL
                     GDEFVKRQLAGASEAGERRYPCNILNWYNVAAEDDFTCHDETVANDFSQMLDYHLVSR
                     IRDFRIYNLALRYGRSNPHNALGYLVHPRVSSLVGEWLLADLS"
BASE COUNT         1409 a         2263 c         2166 g         1357 t
ORIGIN      
        1 cccgttgacc acgtaggaat cgccgtcaag gcgtgccgtg gttttcaggc tggtggtatc
       61 cgtacccgtg gtcggctcgg tcactccgaa gctctgcagg cgcagctccc cggaagcgat
      121 tttgggcagg tatttccgtt tctgctcgtc gtttccgtga cgcagtaccg aacccatcac
      181 gtacatctga gcgtggcacg cgcctgcgtg ggcgcccgat cggcagacct cttccataac
      241 cgccgcggcc tcgagcacgc cgagcccggc tcccccgtac tcctccggaa tcagcacggt
      301 gaggaacccc gacgtggtca gctctttgac gaactcggtg ggatagccgt tgattcgatc
      361 gagttccagc cagtaatcct cgccatactg cgcacacagc tgcctgacgg cattgcggat
      421 ctccgcgagc tcgttttcct ttgacatcgc acttccccgg aaccaggcgg aacgatactg
      481 catgcgacac cgcgattcac gttccagcgc caactcggtc ccgcgctccc gcaaaacgat
      541 gaggccaccg gacgtcgcgc agccagttaa caaactttta caacctccat ttttataaat
      601 taattcatta ttttcattag gttacgaatt ttttctcaat cattttctgc tcatcgcaaa
      661 tccatggtat ttgggacgtt tccgcagcct tctgtgacac atttgcacca tggctaaaac
      721 gtcaaaacat ctgatcggat caatggccgt cgttctggca atgggttgcg ccgcaaatgc
      781 catcgccgac gaagaagccg ttgactccct gcagttcgag gaaacgttcg ggttcgacat
      841 cagccagtat cagctcgacg attacaccga ggtaaccggg tggagactgt ccgacaggtg
      901 gtatttcggc cgtcagaagg gggtcgactc gggtctgggt ttcgtctggc aggacgaggc
      961 tgaccagatg agcatatcga ccaacggcat ccgcttcacg cgccgtttct gagtatccgg
     1021 tccgcctctc agggagggcc ttccggtccc ccacagccgg tcgtccgcgt tacccgatac
     1081 gggtagtatt agctcgtaat cccctacgga gcccgtcgat gcccgtcacc attcctgacc
     1141 ccgatccgaa caacgcctgg cggctcgaga ctccaggcag ccaaggctgg acgcgcaccg
     1201 cacgtgcaac cgaccccgat cgttacttcg tcgtctccgc agacggccat gtgcaggagc
     1261 catcggatct gtggcgaacg cgcatggacg cgaagtaccg cgatcgcctg cccggtgtcg
     1321 ccatggacgc taagggccaa aagttccaga agacggaggg ctttcggcct ctgcggctgc
     1381 agaacatcaa gttcgaaggt gaggacgctc tgcgcaacca atccggccat acacccgaag
     1441 agcgtctggc cgatcttgca gcggacggcg tcgattgcga aatcctgttt ccgaacaaag
     1501 ggctgaccat gtgggccaca ccggatgccg ccttcagcca ggccatgtgc cgggtgttca
     1561 acgattgggc atgggaagtt ttcggccctt acaacgatcg gctcgcgccc atggcctgcg
     1621 tggcgagcgc cgacataccc ggagcagtcg aggagattca gcgctgcgcc aggttgggct
     1681 ttcgcggcgt gacccttccc tgcaaaccgg tgtggggcgc tccggatcac gaggcgctca
     1741 actacaacct gccggatttc gatccgctgt gggcatgcgt gcaggatgcg ggtctgccgg
     1801 tcaccttcca cgtttccacc gggcgcgatc cgcgcacggc tcgcggcaat ggcggcgccg
     1861 tcatcaacta cgccgtgcat tcgctggccc cgaccatgga gcccatcgcc aacatctgcg
     1921 cgtccggcgt gatcgatcgt ttcccgaagc tccggttcgg aaccatagag gcgggcatcg
     1981 ggtgggtgcc ctggaccctc gcggcgctgg acgaagccta caaaaaacac cacatgtggg
     2041 tgaggccgaa gctcaagatg ctacccagcg actacttccg gcagaacggc ttcgccagct
     2101 tccaggagga tcagcccggg ctggacctcg cccgcgagca tcggctgatc gacaattttc
     2161 tgtgggcaaa cgactatccc caccacgagg gcacatggcc tcattcggcc gaggccatcg
     2221 agcgaaccat gggccacctg acggattccg agagagcaaa gatcttgggt ctcaacgcgg
     2281 caagcatctt cggcttcgag gtcccgcggg ggaaggccta ggagtcaggc gtccaggcgg
     2341 cgaacgaccg cgtcgcgcgg cggcgggtct ccgcagggcc ccgactgatg gtggactagg
     2401 cgcgcttcgc cgttctcagc gatgaacagg ttcatggcga ccagcaccgt ccctggcaga
     2461 acctcgtaac agaccaccgc aatgccactg ccgagctcca ccgcccgggc gccgtagaac
     2521 tccacctcgg gctgatcggg attagcgagg atctgtcgcc agctgtcgat gaccgactca
     2581 cggcctacaa gcgccggcca cccgggatga atgcagacaa gcgggtgctg ccgtgaccag
     2641 agctgctcca tggcatccga gtcgccggcc gcgaaagcga gatagaacga ctcgttggca
     2701 aacagtgctt cgttcggatg catgatggcg cgagtatcca cgctctggga gaatggctca
     2761 aggcgagttc gcccggcgtc cgtcgttgca aatggagccg gcttttcgtg aaatgggggc
     2821 gactcgggtc ggacttctgc tttgagcacg ctgcacaaac ccacttgcct gatcgctctt
     2881 tttctgagcg tgacgctcat gacgccaccg ggctggacgg cggccaggga aatcggtccc
     2941 aatcactggg ccggcgtgtc tcgcgtcgtt gcgttcggag acgtccacgg cgcgcacgac
     3001 cagatggtca ccgcattgga atccgctggc gtggtcgacg ccgagctcaa ctgggccgcc
     3061 ggcgacacgc atctggtgag cctcggcgat ctgctcgatc ggggcgcgca ttcccggcag
     3121 accatggatc tgctgatgag attgcagagg caggccaagg gagcaggcgg ccgggtgcac
     3181 gtcgtcctgg gcaatcacga gctcatgaat ctgaccggcg acatgcgcta cgtggccgcc
     3241 gatgagtttg cggcgtttgc agacgatgaa gatccgtcgg tgcgcgaggc ggcgcgcgag
     3301 gcgtttgtcg ccgccgcgga ccccgcttcc ggcaaggacc catctgtgga gttcgacgag
     3361 cggtacccgg ccggatactt tggtcaccgg gcgggatttg ccgcggccgg cagctacgga
     3421 gactggctgc tgcagctgcc gctgatggtg gtcgtcaacg agaccgcatt cgtccacggc
     3481 gggctccccg cgctgatcgc cgatcagacg ctcgaagaga tcaaccggca gtaccaaagg
     3541 gaccttggcg agatcatggc gttgggcggc cggctggccg atgcaggatg ggtcagcgcc
     3601 gagcgcgatc tgctgctgca ggacgatgcg gtgctggcgc ggctggaagc cgccgccgag
     3661 ggagacgacg gcgatctttc ccgagcgggc acgcgtttca ttgagctcgt aagaggcccc
     3721 ctcttcggat cccgaggacc gaactggtat cgaggcaccg ccatgtgcca cgcctccctc
     3781 gaagagacgg tgttgaaggc tgcgctgaaa gccctgggag caaagcgcgt ggtcgtgggt
     3841 cataccccca cgtcgaaccg ccgcgttcag cagcgaatgg acggtcgcgc gatccttgcc
     3901 gataccggca tgctccgctc ttactacagt ggccaacccg ccccggtggt gctgaccggc
     3961 gacgatatcg agattctcta ccctctgagt gaaacccgcg ccgatgctcc tgacgtcatc
     4021 gacccgctgg cacgctacgg gctggatcag ccgcggctgg aggccgtcat gagcaatgca
     4081 gccatcgcag aagtcagcac cgccgggacg ggccagcggt ttaccaccgt cacgctgctc
     4141 gacgaaggca aacggtacga gttcgcgttc gaacgcggcc agggttcaga caacgatcgc
     4201 aaggtcggcg cttaccgcct gtcccgcctg ctcgagctca acgtcgtgcc cgtaacggtc
     4261 agccgcaccg tcgagggcgc caagggcgtg ctcatcgccc tgccggagaa gcgcgtttca
     4321 gagatcgagc gggcaaacca gggactgagc cgttcgaact ggtgcagctt cggcaatgac
     4381 taccagctga tgtacgcttt cgacgcgctg attcgcaacg agcagcgtac taccgatacg
     4441 atgatctacg accaggaaga ctggcagctg acgctcatcg gccacgaggc gagctttggc
     4501 aacggcaccg acctgcccgc ctacctggcg gcggtagaaa agatcctacc ggcagccatg
     4561 gccgcgcgca tggcagccat gaacgaagac agcctcacgg ctgccttagg cgaccagctg
     4621 agcaaacggc agattcgcgc gttgctcaag cggagggacc gaatgatcaa agactggcag
     4681 ctccaggctc tgtaatgcgc aagtcgacac gagcatctgc gcccgtcttc atcatcacgg
     4741 tttactacgt cgtcctcctg ggcggcttca tggcggcatc cagctatctg cccggtctgc
     4801 gggtatacct cccggtcgac ggcatctcag aactcatcga gcggggcggc gcctctttcg
     4861 agcccgtcga gatcatcaga accgccatcg tcgaggcacc ccatatggcc cggctggccc
     4921 tgggcatgat cggggccacc ctgttgatgc tgcccatctc ctgggtgtac ttcatcacca
     4981 cgccgaagag cgccgtggat cgctcgtttg cgcagacgat gatcatgctg cccatcatcg
     5041 ttgccggcat tgccatgatc gtacagaaca gcatcgcgct ggcgttcagc ctggccggcg
     5101 tcgtggctgc ggtgcgattc agattcacgc tgacacagcc ctcttacacg ctctatatat
     5161 ttgccgccat aaccattggt ctcggcggcg gcattgccgc tatcgaaatt tcgacggtgg
     5221 tctccattgc cttcgtctac gccaccatgc tcatgtggat gtcggactac ggcgcggata
     5281 tgaataccag cttcttctca tttctgacgg gacgagaccg tcgtgaagaa gatccttagg
     5341 gcttttgcca gtctggcagc ctgtatagcc ttcagtttcc ttcccggatg tggttcccat
     5401 gacgaaacct tcgcgttcgg cggttaccgc ttcgccaccg atacctttca gcagtggaaa
     5461 ctgccgaagt ccctgaggga aatctcgggc cttgccgcca ccgcggacgg gcggctgttt
     5521 acgcacggcg acgagagagc cgagatctac cagatcgact accgggacgg caagctggtg
     5581 aagtcgttca ggctgggaat ctcgtcggac agcaggccgg tcaagggcga tttcgaaggc
     5641 atcgccgtac gcgacagcga gctatacctg attaccagca acgggatcct ctaccgctca
     5701 cagatcggcg gcgacgggtc gacgatgcca taccagacct tcaattcagg cgcgggggat
     5761 ctgtgtgagg ttgagggcct ggcctaccgc ccgcaaacgg acatgctgct gatcgcgtgt
     5821 aaagaggttc gcaaggtgga gttcgaggac aaggtcgtcg tgctgcagtg gtctccgcag
     5881 actcggcgcc tggagcgcga catgagcatc ctgatcaatc aatcgtccac gggtggccga
     5941 ttcagcgctt ccggaatcag cgtttcgcca cgcaacggta atctgctact ggtctccgct
     6001 cggcagcgaa ggctccttga ggtgacacct gaagcccacg tcgtcgccgt attatcgctt
     6061 cccatggaac gctatcatcg acaaactgag ggcatcgagg tgacgggcag cggaaccctc
     6121 gttctagctg acgagggcgg aaataaaagg ggccgacttg gaacttatcg aggcagaaat
     6181 taagccgccc aagaccatca tcatggtcca cggccgcggc tggaagcctg caaggcaagc
     6241 gctcgagtca ctctgggtcg aatctctacg gaccggcctg caacgtgacg cggaaagcca
     6301 gtcgacaagc gttgcgctgg atatggcgaa gatccagatg gtctattatg gcgacctgac
     6361 caacgccatc agggccgggc gcggaaaagc ctatgacgag gccctcgacc ttgcggacct
     6421 gaccaacgcg ctggatttcc tgaaagcgct gcacacagcc aagaagtttc gacgcgcccg
     6481 ttacgaagcc cttcctggtc gcagctcgct ggaggagttt ttggccgaca tcggcgcacc
     6541 gattctctcc acactgcggc tgaccgacaa agtcatcggc cggcgaatgc cggaggtcgt
     6601 cgcttactgg gacgatcggg acggtttcag ctcccaaatc tgcgcgaggc tcttgaacga
     6661 gatccgaaaa gccaaggacg aagattccga cattctcgtc atttcccatt gcctcggcac
     6721 gatcgcggcg tatgacgcct tctgggaact ctcgcgcggc caagatgccg acccgagcgc
     6781 aaagatcgac acctggatca ccatgggctc tcccctcgga gatgagttcg tcaaacgaca
     6841 actcgccggc gcctctgaag ccggcgagcg ccggtacccc tgcaacattc tcaactggta
     6901 caacgtcgct gcagaggacg atttcacctg ccatgacgag accgttgcca acgacttcag
     6961 ccagatgctc gactaccacc tggtcagtcg aatacgcgac ttccggatct acaatctggc
     7021 gcttcgatac gggcgctcga atccgcacaa cgcgctcggc tacctcgtcc accccagagt
     7081 gtctagcctg gtcggggaat ggctgcttgc cgatctgagc tagcgcagac gccgacaggc
     7141 ttccgctatg ttctcttcaa acgactcgga gtcgtccaga tcgccataaa actcg
//
LOCUS       DBNHLM010000244         4998 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_633885, whole genome shotgun sequence.
ACCESSION   DBNHLM010000244 DBNHLM010000000
VERSION     DBNHLM010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_633885"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="AC16QF_11295"
     CDS             complement(<1..425)
                     /locus_tag="AC16QF_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR6449186.1"
                     /translation="MFRRLSELGVLGRAIDRGIVTLELFNPREFARDRHRTVDDRPYG
                     GGPGMVMQVEPVVAAVSAAREAARAALGESVPVIYLSPQGRRLTQQRVSELSQQRGVI
                     LLAGRYEGIDERAVSLAVDEEVSIGDYVLSGGELPALVVL"
     gene            complement(461..964)
                     /gene="rimM"
                     /locus_tag="AC16QF_11300"
     CDS             complement(461..964)
                     /gene="rimM"
                     /locus_tag="AC16QF_11300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR6449187.1"
                     /translation="MHSQEYVIVGRFGGPYGVHGWIRVTSFTDPPANLLLYGPWHVER
                     DGQWHAYHPVQVRPHQQGLIACYADIPDRSVAAALTGRFIAVPRDCLPVLDAEEYYWR
                     DLEGLEVWNRGARLGVVDHLIDTGANSVLAVRGDSTQTLIPFVSRFVTGVDLAAGRID
                     VDWEEVD"
     gene            complement(1021..1293)
                     /gene="rpsP"
                     /locus_tag="AC16QF_11305"
     CDS             complement(1021..1293)
                     /gene="rpsP"
                     /locus_tag="AC16QF_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR6449188.1"
                     /translation="MLTIRLARHGSKKNPFYHITVADRTAKRDGRFVERVGFYNPVAK
                     GQAEELRVDLERIDYWLSVGALASDPVTRLIRRARRQEAAVEATAD"
     gene            complement(1392..2756)
                     /gene="ffh"
                     /locus_tag="AC16QF_11310"
     CDS             complement(1392..2756)
                     /gene="ffh"
                     /locus_tag="AC16QF_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR6449189.1"
                     /translation="MFDNLSERLTQTLRGISGKAKLTEENVQDALRDVRMALIEADVA
                     LPVVKDFLSQVQTRALGQTVSSALNPGEEFVRIVHEELVHVMGDDNDALNLATTPPAV
                     VLMAGLQGAGKTTTVAKLALLLKERERKKVAVVSADVYRPAAIEQLATLAGEVGVICV
                     PSSAQEKPLEIARRAVDEARNQFADVLLVDTAGRLAIDADMMAEIKLLHEELQPIETL
                     FVVDAMTGQDAANTAKAFNDALALTGVVLAKADGDARGGAALSVRAITGKPIKFIGMG
                     ERVDALEPFYPDRMASRILGMGDVLSLIEEAERKVDKRKAQKLAKKIQRGRKFDLEDF
                     REQLDQMSNMGGVSSMLEKLPGMGQLPPAAQAQLDDGMFRKMAVIIDSMTPHERRFPD
                     VITGSRKQRVARGSGTQVPDVNRLLKQHKQMQRVMKKMSRKGGMQRMMRGMQGMRPPP
                     GVRR"
     gene            2925..3695
                     /locus_tag="AC16QF_11315"
     CDS             2925..3695
                     /locus_tag="AC16QF_11315"
                     /inference="COORDINATES: protein motif:HMM:NF013725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C assembly family protein"
                     /protein_id="HIR6449190.1"
                     /translation="MLLYLLGTAMQLRRLSRSAPVLLQPLIAVGIPALVLHALTTISV
                     IYTPAGIHLGFFSTGGLVTLVMTAFVMLASLRLPVHNLCVLIFPASALGIIGSLLGES
                     AFVPRQEMPWELLLHVLISITAYSILFMAACQSVLLAIQEHYLRARHGLAFIRLLPPL
                     ETMETLLFSMLWTGLVVLTVAILSGFAFLEDMFAQRVVHHTVLSSLSWLLYATLLAGH
                     KLLGWRGTTAVRWTLIAFLLLVLGYFGSKFVIEILLDA"
     gene            3695..4966
                     /locus_tag="AC16QF_11320"
     CDS             3695..4966
                     /locus_tag="AC16QF_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HIR6449191.1"
                     /translation="MSLTELPASVLLIFVFVLTALSAYFSSSETAMIALNRYRLKHLV
                     KQKHRGARKANRLLRRPDRLLGVILIGNNLVNFTAASIATVIGLRFFGDTGVVLAPIV
                     ITVIFLIFAEVAPKTIAAKVPERIAFPSAYLLEPLLRLLYPGVVFINGISNAIVKPFL
                     PKEGEIAADSLSVEELRTVVGEGVGLPGQGQEMLLSILDLEKVSVDDIMVPRSEIVGI
                     DVDADIGDILVQIGNSQHTRLPVFKENINNILGILHLRKVARYLQRKEREEELSKSDL
                     MSLTDEPYFVPEATPLPTQLINFQNEKQRIALVVDEYGEVQGIVTLEDILEEIVGEFT
                     TDFAASIQEIHPQDDGSFFIDGMALLRDINRSLGWDLPTHGPKTLNGLVLEHLETLPD
                     SNLCLRLGEYLIETLHIKENAIKTLKITRVE"
BASE COUNT          868 a         1672 c         1507 g          951 t
ORIGIN      
        1 agcacgacca gcgcgggcaa ctcgccgccg ctgagaacgt agtcgccgat ggaaacttcc
       61 tcgtccaccg ccagcgagac ggccctctcg tcgatgccct cgtaacgacc cgccagcagt
      121 atcaccccgc gctgctgaga caattcactg actctctgct gcgtcagccg ccgaccctgg
      181 gggctcaggt agatcaccgg cacgctctca cccagggcgg ctcgggccgc ctcccgcgcc
      241 gcgctgacgg ccgcaacgac cggctctacc tgcatgacca tcccgggccc gccgccgtat
      301 ggccggtcat ccaccgtgcg gtgtcggtcc cgggcgaatt cgcgcgggtt gaagagctcg
      361 agggtcacga tgcctcgatc gatcgctctg ccgagcacgc ccagctcgga gagccgtcgg
      421 aacatctccg gaaaaagact gacgatgccg caccacacgt ctagtcgacc tcttcccagt
      481 cgacgtcgat cctgcccgcg gcaagatcga ccccggttac aaaccggctg acgaaaggaa
      541 tcagcgtctg cgtcgagtcg cctcgaaccg ccagcaccga gttcgcgcca gtgtcgatga
      601 gatgatccac cacgccgagc cgcgccccgc ggttccatac ctcgagacct tccaggtctc
      661 gccagtaata ctcttccgcg tccagcaccg gcagacagtc ccggggtacg gcgatgaacc
      721 gcccggtgag cgcggccgcc acgctgcggt ccgggatgtc tgcgtagcag gcgatcaagc
      781 cctgctggtg gggccgcact tggaccgggt ggtaggcgtg ccattgcccg tcacgctcca
      841 cgtgccaggg cccgtagagg agaaggttgg cgggcggatc cgtgaacgag gtgacccgga
      901 tccagccgtg cacaccgtaa ggaccgccga agcggccgac gatgacgtat tcctgggaat
      961 gcatgcggaa gactccggga tccccggcaa cgcccgccgt ggggcgcccg acgcggcccg
     1021 tcagtcggcg gtggcttcca cggcggcttc ctgccgccgt gcccgacgaa tcagccgggt
     1081 gaccggatca gacgcgagcg cgccaacgga cagccagtag tcgatccgct cgagatcaac
     1141 gcgcagttcc tcggcctgac ccttggccac cggattgtag aagccgaccc gctcgacgaa
     1201 tctgccgtct cgcttcgccg tgcgatccgc gaccgtgatg tgatagaagg ggttcttctt
     1261 ggatccgtgg cgtgccaggc gaatcgttag catgtgatga ccctgctgcg ggcccgccag
     1321 gcgggcccaa tcgttgaaag gcgcggtatt ttaatggaac gcccggctca tgcaaggccg
     1381 gtgaggctcc atcaccgacg cacgccgggc ggcggccgca tcccctgcat gccccgcatc
     1441 attcgctgca tgccgccttt gcgcgacatc ttcttcatca cccgctgcat ctgcttgtgc
     1501 tgtttgagca ggcggttcac atccggcacc tgagtgccgc tgccccgcgc gacccgctgc
     1561 ttgcgggatc cggtgatcac gtccgggaag cggcgttcgt ggggggtcat ggagtcgatg
     1621 atcaccgcca tcttgcgaaa catcccgtcg tccagctgag cctgcgccgc gggcggcagc
     1681 tgccccatgc ccggcagctt ctccagcatg ctcgaaacgc ctcccatatt ggacatctga
     1741 tcgagctgct cgcggaagtc ctccaggtcg aacttgcgac cacgctgaat cttcttggcg
     1801 agtttctgtg ccttgcgctt gtcgactttg cgctcggctt cctcgatgag gctgaggaca
     1861 tcgcccatgc cgaggattcg cgatgccatg cggtccggat agaaaggctc cagggcatcg
     1921 acccgttcgc ccatgccgat gaacttgatg ggcttgcccg tgatggcgcg gaccgacagg
     1981 gcggcaccgc cccgggcatc cccgtcggcc tttgccagca cgacgccggt tagcgccaac
     2041 gcatcgttga aagccttggc cgtgttggcc gcgtcctgac cggtcatggc gtcaacgacg
     2101 aaaagcgtct cgatgggctg gagctcttcg tgcagcagct tgatctccgc catcatgtcg
     2161 gcatcgatcg caaggcgtcc cgcggtgtcc acgagcagaa cgtcggcaaa ctggttgcgg
     2221 gcttcatcaa cggcacgacg ggcgatctcc agcggcttct cctgcgcgct ggatggaaca
     2281 cagatcacgc cgacctcccc ggccagggtc gcgagctgct cgatggcagc cggacggtaa
     2341 acgtcggcac tgaccaccgc caccttcttg cgctcccttt ctttcaggag cagcgcgagt
     2401 ttggcaaccg tggtcgtttt gccagcgccc tgcaggccgg ccatgagcac gacggccggg
     2461 ggcgtggtgg ccaggttgag ggcgtcgttg tcatcgccca tcacgtggac tagctcttcg
     2521 tgaacgatcc gaacgaactc ctcgccggga ttgagagcag acgaaaccgt ctggcccagg
     2581 gccctggtct ggacctggga gagaaagtct ttcaccaccg gcaacgcgac gtcggcctcg
     2641 atgagggcca tcctcacgtc gcgcaaggcg tcctggacgt tttcctccgt gagcttcgcc
     2701 ttgcccgaga tgccgcggag cgtctgggta agtcgttcgg aaagattgtc gaacatgaaa
     2761 acctcgtcga gccgttggac gcacgccggg accatcggcg gcgggccgat tatagccctt
     2821 cacgctattg gtgcgttatg ccaaactccc cgacgctcca gccatcgacg acgccgaccg
     2881 aaatggttgc cacaactacg agcatcgccg ccggaacgtg cgccgtgttg ctgtacctcc
     2941 tgggcaccgc gatgcagctg cgacgactgt cgcgatcggc tcccgtgctg ttgcagccgc
     3001 tcatcgcagt ggggatcccc gctctggttc tgcacgccct aaccacgatc agcgtgatct
     3061 acactccagc gggaattcac ctcggttttt tcagcaccgg cggcctggtc acgctggtga
     3121 tgacggcatt cgtgatgctg gcctccctca ggctgcccgt tcacaacctg tgcgtcctga
     3181 tatttccggc cagcgctctc ggcatcatcg gctcgctgct cggagaatcc gctttcgttc
     3241 cgcggcagga gatgccctgg gaactgctgc tgcacgttct catctccatc acggcctaca
     3301 gcatcctgtt catggccgcc tgtcagtccg ttctgctggc gattcaggaa cactacctgc
     3361 gagcgcgcca cggcctggcg ttcatacgtc ttcttccgcc gctggagacc atggagacgc
     3421 ttctgttttc gatgctctgg accggcctcg tcgttctcac ggtggccata ctgagcggtt
     3481 tcgcctttct cgaggacatg ttcgcgcagc gcgtcgtcca tcacacggtg ctctcgtccc
     3541 tgtcctggct gctctacgcg accttgctgg cagggcacaa gctgctggga tggcgcggca
     3601 cgacggccgt tcgctggacg ttgatcgcct tcctgctgct cgtgctaggc tacttcggta
     3661 gcaagttcgt catcgagata ctgctggacg cctgatgtcc ctcaccgagt tgcccgcttc
     3721 cgtcctgctg atcttcgtat tcgtgctgac cgccctgtcg gcctattttt ccagttccga
     3781 gaccgcgatg atcgctctga accggtatcg cctgaagcat ctcgtgaagc agaagcaccg
     3841 cggcgcccgc aaggcaaacc ggctgctgcg gcgtcccgac cggttgctcg gcgtcattct
     3901 gatcggcaac aacctggtga acttcacggc cgcgagcatc gcgaccgtga tcggcctgcg
     3961 ctttttcggt gataccggcg tcgtgctcgc ccccatcgtc atcaccgtga tcttcctcat
     4021 tttcgccgag gtagcaccca agaccatcgc cgccaaggtt cccgagcgga tcgcctttcc
     4081 ctcagcgtac ctgctcgagc cgctgctcag gctgctgtat cccggcgtgg ttttcatcaa
     4141 cggcatttcc aacgccatcg tgaaaccctt cctgccgaag gagggcgaaa tcgccgcgga
     4201 tagcttgagc gtggaggagc tccgcaccgt cgtgggagaa ggcgtcggcc tgcctggtca
     4261 gggacaggaa atgctgctga gcatcctgga cctggaaaag gtttccgtcg acgacatcat
     4321 ggtgccgcgc agcgagatcg tcgggatcga cgtcgatgcc gacatcggcg acattctcgt
     4381 ccagatcggc aacagccagc acacgcggct gccggtcttc aaggaaaaca tcaacaacat
     4441 cctgggcatt ctgcatctgc gcaaggtcgc ccgctacctg cagcgcaagg agcgcgagga
     4501 agagctgagc aaaagcgatc tgatgagcct gacagacgag ccgtacttcg tccccgaggc
     4561 gacgcctctg cccacccagc tcatcaattt ccagaacgag aagcagcgga ttgccttggt
     4621 ggtggacgag tacggcgaag tgcagggcat cgtcactctc gaggacatcc tcgaggagat
     4681 cgtcggcgag ttcacgacgg actttgctgc gagcatccag gaaatccatc ctcaggacga
     4741 cggctcgttc ttcatcgacg ggatggcgct tctgcgggac atcaaccgga gcctgggctg
     4801 ggatctgccc actcatggcc ccaagaccct caacggcctg gttctggaac atctggaaac
     4861 gttgcccgac agcaacctct gcctgcggct gggcgagtac ctgatcgaga ccctgcacat
     4921 caaggaaaac gcgatcaaga cgctgaagat tacccgggtg gagtgagtcg tcggccacca
     4981 ccggggcgag gcccgcca
//
LOCUS       DBNHLM010000245         3311 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_453593, whole genome shotgun sequence.
ACCESSION   DBNHLM010000245 DBNHLM010000000
VERSION     DBNHLM010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_453593"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="AC16QF_11325"
     CDS             complement(<1..1039)
                     /locus_tag="AC16QF_11325"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HIR6449192.1"
                     /translation="MRKAVYLDYAATTPVDPRVAQKMSACLLVEGNFGNPASRSHVYG
                     WQAEEAVEHARTQVAKLINADPREIVWTSGATESDNLAIKGVADGAVKRHIVTSAIEH
                     KAVLDTCKYLETKGFEVTYLDPGPDGIVEPGQVHDVLRPDTLIVSIMHVNNEIGVIND
                     IASIGAVCREAGVLMHSDAAQSGGKVAIDVRAMPVDMISLSAHKMYGPKGMGALYVRR
                     EPPLKLTPLIHGGGHERGMRSGTLATHQIVGMGEASAILMRDMERENARITSLRQRLW
                     ERLGKIPGTRLNGDETRRVPGILNVSFPGVDGETLLMALDDVAVSSGSACTSASVEPS
                     YVLRALRVPDEL"
     gene            complement(1039..1497)
                     /locus_tag="AC16QF_11330"
     CDS             complement(1039..1497)
                     /locus_tag="AC16QF_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HIR6449193.1"
                     /translation="MRLTTKGRYAVTAMLDIALHRDAGPVSVVDIAHRQDISAAYLEQ
                     LFSKLKRAGLISSIRGPGGGYQLALDAEAINVSRIIAAVGEGVDATRCHGAGDCQDGL
                     TCLTHDLWTELSQEIDSFLGGITLASLVRKRGVRSIASRQDSQLIEARLI"
     gene            complement(1556..2293)
                     /locus_tag="AC16QF_11335"
     CDS             complement(1556..2293)
                     /locus_tag="AC16QF_11335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005760054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HIR6449194.1"
                     /translation="MSEVLKGIRIVLVETSHPGNIGGAARAMKTMGLSDLVLVNPKRY
                     PDPQAQWRAAGAQDVLEAARVCADLTEAIEGCYWVVGTSTRSRRIPWPVLSPEEVARQ
                     AVERAGSSIAILFGREANGLSNEELQRCHCHLQIPAAPDYPSLNVAMAVQVVCYELHK
                     LAGEAGDEIGAWDRPTATVAQLDGLIEHLEEVMVASGFLDAANPGQTMTRLRRLFSRM
                     EPDTTEVQILRGILTHLHPLRRPGPGE"
     gene            2400..3200
                     /locus_tag="AC16QF_11340"
     CDS             2400..3200
                     /locus_tag="AC16QF_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR6449195.1"
                     /translation="MQAMANMALRAARQAGQIMTRAMDRVDLLQVEEKARNDLVSNID
                     REAEQAIREVLLKAYPEHGILGEEYGETNPEAEYVWVVDPLDGTTNYLQGIPHFCISI
                     ACMRGRQIEHAVIVDPVLNEEFVASRGSGAQLNGKRIRVSRTEKLRDAVVGTGIPPKA
                     IARHLEPYMAMLHDFVGTCRAIRRSGSAALDLAYVAAGRLDGFWELALSPWDIAAGTL
                     LVREAGGFVGDLSGGDAFMQHGNIVAANPKCFKAMVTRMRPNLTPELA"
BASE COUNT          603 a         1114 c         1010 g          584 t
ORIGIN      
        1 cgagttcgtc tggcacccgc agcgcccgca gcacgtagct tggctcgacg ctggccgaag
       61 tgcacgcgga cccgctcgag acggccacgt catccagtgc catcaaaagg gtctcaccat
      121 ccacgcccgg gaagctcacg ttgaggattc ccggcacccg gcgcgtctca tcgccattca
      181 gacgggttcc cggaatctta ccgagccgct cccacaaccg ctgacgcagg gacgttatgc
      241 gggcgttctc gcgttccatg tcgcgcatga gaatggcgct agcttctccc atgccgacga
      301 tctggtgggt cgccagggta ccggagcgca ttccgcgctc gtgaccgccg ccatgaatca
      361 gcggcgtcag cttcagcggc ggctcgcgac gcacgtagag cgcacccatg cctttggggc
      421 cgtacatctt gtgcgccgac agggaaatca tgtccaccgg catggcgcga acgtcgatcg
      481 caaccttgcc gccgctctgg gcggcatcgc tgtgcatgag cacgcccgcc tcccgacaga
      541 ccgcaccgat cgaagcaatg tcattgatca cgccgatctc gttgttgacg tgcatgatgc
      601 tgacgatgag cgtgtcggga cgcagcacat cgtgcacctg ccccggctcg acgatgccat
      661 ctgggcccgg atccagatac gtcacctcga aacccttggt ctcgagatat ttgcaggtgt
      721 ccagcacggc cttgtgctcg atggcgctgg tcacgatgtg ccgtttcacc gcgccgtcgg
      781 caactccttt gattgccagg ttgtcggact ccgtcgcgcc ggaggtccac acgatttccc
      841 gcggatcggc gttgatgagc tttgcgacct gggtcctggc atgctccacc gcctcctcgg
      901 cctgccagcc gtagacgtgg gagcgagacg ccggattgcc gaaatttccc tccaccagca
      961 ggcaggcact cattttctgg gcgacccgcg gatccaccgg ggtggtcgcg gcgtaatcca
     1021 gatagacggc cttgcgcatc agatcagccg cgcttcgatc agctggctgt cctgacgcga
     1081 agcgatgctg cgcacacctc gtttccgcac caggctggcc agggtgatgc cgccgagaaa
     1141 cgagtcgatc tcctgagaaa gctccgtcca aagatcgtgc gtcaggcagg tcaacccatc
     1201 ctgacagtcc cccgcgccgt gacagcgtgt ggcgtccacg ccttccccca ccgccgcgat
     1261 gatccgggac acgttgatcg cttcggcgtc gagagcgagc tgatacccgc cgcccggacc
     1321 gcgaatgctg gagatgagcc cggcgcgctt cagcttgctg aacagctgct cgagataggc
     1381 ggcggagata tcctgacgat gagcgatgtc cacgacgctc accgggccgg catcgcgatg
     1441 cagcgcaatg tccagcatag ccgtgaccgc atatctgcct tttgtcgtca gccgcatgga
     1501 tggcccacca tcgatacaag ccaggcagta tgcaataccc gactaaaatg gtccactatt
     1561 ccccggggcc gggacgccgc aagggatgca ggtgggtaag gatcccgcgc agtatctgga
     1621 cctccgtcgt atccggttcc atcctggaga aaagccggcg caggcgggtc atggtctgtc
     1681 ccggattcgc ggcgtccaga aagccgctgg cgaccatcac ctcttcgaga tgctcgatca
     1741 gcccgtcgag ctgagcaacc gtcgcggtgg ggcgatccca ggcgccgatt tcgtctccgg
     1801 cctcgccggc cagcttgtgc agctcgtagc acaccacctg caccgccatg gcgacgttga
     1861 gcgaggggta atcgggggcc gcgggaatct gcaggtgaca gtgacatcgc tgcagctcct
     1921 cgttgctgag cccattggcc tcccggccga acaggatggc aatggagctg cccgcccgct
     1981 cgaccgcctg cctggcgacc tcctccgggg acagcacggg ccagggaatg cgccggctgc
     2041 gggtgctcgt gccaaccacc cagtagcacc cctcgatagc ctcggtgaga tcggcgcaga
     2101 cacgagcggc ctccagcacg tcctgggcac ctgccgcgcg ccactgcgcc tgcggatcag
     2161 ggtagcgctt cgggttgacg agaacgaggt cggaaagacc catggtcttc atcgcccgcg
     2221 cagcgccgcc gatgttgccc ggatgactcg tctccacgag gacgatgcgg attcccttca
     2281 atacctcaga cacaatcggc ttccagtgct caacagcaat cggccatttt ccgacattcc
     2341 ctacctgcaa gcacgttgtt aggatagccg ccctcctctt tggtaccgct ttcaacacca
     2401 tgcaggcaat ggcgaacatg gctctgcgcg cagcgcgcca ggccgggcag atcatgaccc
     2461 gggcaatgga tcgcgtcgac ctcctgcagg tggaggagaa agcacgcaac gatctggtat
     2521 ccaacatcga ccgggaagcc gagcaggcca ttcgggaggt gctcctgaag gcctaccccg
     2581 agcacggcat cctgggcgaa gaatacggcg agaccaaccc cgaggccgag tacgtctggg
     2641 tggtcgaccc gctggacggc accaccaact accttcaggg aatcccgcac ttctgcattt
     2701 ccatcgcgtg catgcgagga cgtcagatag agcatgcggt gatcgtcgac ccggtactta
     2761 acgaggagtt cgtcgccagc cgcgggtccg gcgcacagct gaacgggaaa cgcatacgcg
     2821 tcagcagaac cgagaaactt cgcgacgcgg ttgtcgggac cggcatcccg cctaaggcca
     2881 tcgcgcggca cctcgagccc tacatggcga tgctgcacga tttcgtcggc acctgtcgcg
     2941 ccatacgacg cagcggctcc gcggcgctcg acctggccta cgtcgcggcc gggcggctcg
     3001 acggattctg ggagttggcg ctgagcccct gggacatcgc cgccggcacg ttgctggtgc
     3061 gcgaagcggg aggtttcgtc ggcgatctct ctgggggcga tgccttcatg cagcacggca
     3121 acatcgtcgc ggccaacccg aaatgcttca aggccatggt tacccgcatg cgcccgaacc
     3181 tgacccccga gctcgcctga tcagggcacc gcctggtccg gattctcctt ctccggtatc
     3241 agcaggtcct cgcgggtgat gttcatcacc agaagcacgt tggccgccac gtaaatcgac
     3301 gagtaggtcc c
//
LOCUS       DBNHLM010000246        15931 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_573541, whole genome shotgun sequence.
ACCESSION   DBNHLM010000246 DBNHLM010000000
VERSION     DBNHLM010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 15931)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15931)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15931
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_573541"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1443)
                     /locus_tag="AC16QF_11345"
     CDS             complement(85..1443)
                     /locus_tag="AC16QF_11345"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020800276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HIR6449196.1"
                     /translation="MLSWNDFEEEHANAETAMQQPPAEAPLESKEDAAASFPPPGEAV
                     AGAGAVAEAAAGAAAPAPRPVEELPPITVSEPAAPLSEAPSNDEVLQRAEAAIGEMGL
                     EGLAEDGIEENYQRVTVDQKRMINCRADLNQLVPFKYDWAWQKYLDGCANHWMPQEIN
                     MTADIALWKSESGLTQDERLVVKRSLGFFSTADSLVANNLVLALYRLITNPECRQYLL
                     RQAFEEAIHTHAYQYCIESLGMDEGEIFNMYHEVPAVARKAGWALKYTRDISDPTFQT
                     GTPQADKALLKNLIAYYCVLEGIFFYCGFTQILSMGRLNKMTGTSEQFQYILRDESMH
                     VNFGIDVINQIKLENPGLWDEELQTLARQMILEGTQLEIEYAKDTMPRGILGMNANMM
                     AEYLQFIANRRLAQIGLAEQFAGAKNPFPWMSEIMDLKKEKNFFETRVTEYQTGGALS
                     WD"
     gene            complement(1457..4339)
                     /locus_tag="AC16QF_11350"
     CDS             complement(1457..4339)
                     /locus_tag="AC16QF_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HIR6449197.1"
                     /translation="MATAQPLPPSIAATAPGEIKVIKRNGAVVPYNDDKIAVALTKAF
                     LAVEGGTAAASPRIRELVAELTLQVSATFKRRFPSGGTIHIEDIQDQVELVLMRSGEH
                     KVARDYVLYREERARARAADVVQPASNALNVVQEDGSREPLDLDRIRKLVGEACVSLS
                     DVEADRIIDEAMSNMYDGISAKDVATALQIAARTLVEEEPNYTYVTARLLLDELRTEA
                     FRFLEVNAEGEHRATQSEVGGYYPAALAAFIEKGIELQLLDPVLATFDLAALGGAIKS
                     ERDLKFTYLGLQTLYDRYFIHSNDVRFELPQVFFMRVAMGLAVQEDDINARAIEFYDL
                     LSSFDYMSSTPTLFNSGTLRPQLSSCFLTTVPDDLEGIYGAIRDNAMLSKWAGGLGND
                     WTPVRALGSYIKGTNGKSQGVVPFLKVVNDTAVAVNQGGKRKGAVCSYLETWHLDIEE
                     FLELRKNTGDDRRRTHDMNTANWIPDLFMQRVTEGEDWTLFSPSDVRDLHDLYGQAFA
                     KRYEQYEEMTRTGELKLFKRVKAPELWRKMLSMLFETGHPWITFKDACNVRSPQSHVG
                     VVHSSNLCTEITLNTNGEEIAVCNLGSVNLVQHVRDGELDTRKLKQTVRTAVRMLDNV
                     IDINYYSVPQARNSNMRHRPIGLGIMGFQDALYKLRIPYASEDAITFADRSMEAVSYY
                     AIDASCKLAKERGAYASFSGSLWSRGILPIDSLKQLHQERGAQYLDANFDATMNWDKL
                     RGRVQINGMRNSNVMAIAPTATIANITGVSQSIEPTYQNLYVKSNLSGEFTVVNPYMV
                     HDLKDRGLWDKVMVNDLKYYDGSVLSIDRVPDELKQLYATAFEVEPRWLVDAASRRQK
                     WIDQAQSLNLYIAGASGRKLDVTYRMAWLRGLKTTYYLRSLGATSTEKSTLDRGRLNA
                     VSATQADPTTAARAPTPLDMDSVTAGGPAQVPLACSIDDPDCEACQ"
     gene            4914..6392
                     /gene="glpK"
                     /locus_tag="AC16QF_11355"
     CDS             4914..6392
                     /gene="glpK"
                     /locus_tag="AC16QF_11355"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIR6449198.1"
                     /translation="MSHLLALDQGTSSSKAIIFNLTGRVVGVGQHEFDNTFPADGWVE
                     QDPEVLWRTTLLAGREALASAGLKGEDIAAIGITNQRETTLVWDRQTGRCPHNAIVWQ
                     DRRTADRCELMRLDGLAEAVAETTGLLIDPYFSATKLAWILDNVSGAREQAESGALAF
                     GTVDTFLVWRLTKGRHHATDASNASRTMLFDIAGQTWSERLLNYFNIPAAMLPAVRDS
                     ADDFGVADPEWFGAPIPICGIAGDQQAALIGQGCFNPGMAKSTYGTGCFAMINTGAEQ
                     LRSQQQLLTTVAYRLDGEITFALEGSIFSAGVAVKWLRDQLGLVVDAADTEAAAIRAQ
                     GDTAGVFLVPAFTGLGAPHWAPEARGLVSGLTLATTRDQLITATLQAVAFQSHELIAA
                     MASDGAVVDNLRVDGGMVVNDWLCQCLADVVNLPVERPEITETTALGAAILAALGAGL
                     VEDLDSAARLWNLEREFLPAMDPARRESLLKGWRAAVARTLL"
     gene            6572..7624
                     /locus_tag="AC16QF_11360"
     CDS             6572..7624
                     /locus_tag="AC16QF_11360"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR6449199.1"
                     /translation="MRIVASLVAMTGYLLFCVMLFRFGYLDTGPPVFYTLLTLWSVVL
                     AALVVFDVPSARFDRGYLTFFKALWCNVGVVATALVVPASLRPLMLVVPLFGVLYTAL
                     HLERKHVLAVGTVTWFTYVLGALIQNDMRFIDETSPGLSTEGMVVVAFALMLIVMTVM
                     GGEVTALRNAFAHRRERLKQAMERLSELAMRDELTGLYNRRYIMDALAQQKAVADRGR
                     LGFAVLYCDLDHFKRINDRFGHQSGDRALCDFGKLASSVVRSIDYVARIGGEEFLLVL
                     VGADINDATIVAERLCKRTRNLPISAQGGEYHLTVSIGVSSFRSGERVEDVIQRADLA
                     VYRAKSGGRDQISVLV"
     gene            7693..11655
                     /locus_tag="AC16QF_11365"
     CDS             7693..11655
                     /locus_tag="AC16QF_11365"
                     /inference="COORDINATES: protein motif:HMM:NF019410.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HIR6449200.1"
                     /translation="MENRRRYPRYPISLSALMHPAQGRSWLCTISNFCRDGMLLVGNG
                     GPGSLDATGADAKPGDTVALHFSVALPGGQKHFRTQARVARILGSGEGMGVHFPGGLP
                     DDAYDTLMAFEAAGNPSGENSQPLDEDPPDDVESPQAPVAPQRISHEDADEIRRKMRR
                     ITQPTLDALCQKCFELIEEDLREKTLASLGDGLGEIYDSGRELIEKYRNQIRNDFIGG
                     VLRQISNVAQLEDIAPLRSRREEAIRREHEQAGSDKFDGWLDVAEVVAKAESRYRDDL
                     RDLRARLGLVEPPWRHKEVVPVGPAAITWAFDGALKDLELTPRIRTHVFEGFQAALLP
                     ALNDFYHGLGELLSECEIFPTEEALEAISALQEPARDDDLTLHEYVPGSARHGEASGD
                     EPDAGEEAAVGVKLATHDEPEAEQVGPAPREVYATARSIVKLERVARQQLEGEGGEPR
                     QAAGGGAANPFGSEEILRVLGALGPGGQGDRATVCQRLVQELDAADGDRSLGESDLDR
                     FRVLESLIDSICEDRFLSQGSLNWVRRLEVTLYKLAVHNPGFPGGDPDRPHPAEIMLN
                     QLARLSGIADSREAKARELSGRVDALVERVADGYSGDGGIFEEAVAELNPLVDEQIRN
                     YRGNMERIVRASEGQQRLTRARKAVVNALAPKMAGKEVPEPILKLLNPGWRNLMVHTC
                     LRMGAGSAQWEERVAVIERLWAEFVAEPEQGGRDASDLKQLLRQVADGLISISFDPGL
                     RKPLLLDIAKVLRAKADGKPAPVKTVSVAADGVADALGIARLLPESDAELEAAQQEMQ
                     RSWAKSLSRARTLQAGDWIATDDAQGRPLILVVAFVGDESSGFVLANRNGRKERELTL
                     TEMADNLHEARMTLLSDFDMPLVERAAERMLETVHGRLADQSGRDALTQLVNRREFER
                     RLDKALKRSRADGVDHALMLIDLEGVEAVRSDTGDAGADELLKGAADVLTRALIGRPA
                     DIGRVDADRFGILLERTSGPAAMEIAEQILKALGDDPLRVSSGQQALSASIGLVSMDT
                     QSESANAVMHYAERACRAARLSGRNQIKEFEIGEIAQLRRHGEAAWAFRLDEAMAGEG
                     LIVSFRQIAPMSGARNGVDTHYEVQLTMRDEEGGIMAPEKFQDAAESHERMPALDRWT
                     VAWCLDWMAAHKDSLDHFGGFAIRLSDRSLNEPTFSDFVLERFSACQVPTSKICFEIT
                     ESVAVANIENAVEFMNRMKIIGCRFALEDFGSGRASYAYLRRLPVDYVKIDDVFVREI
                     AANPGDYAVVRSINEIGHYMGKKTIAEFVEDEEVRARLAEIGVDYLQGMDAQSAIPLR
                     "
     gene            complement(11682..12602)
                     /gene="argF"
                     /locus_tag="AC16QF_11370"
     CDS             complement(11682..12602)
                     /gene="argF"
                     /locus_tag="AC16QF_11370"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR6449201.1"
                     /translation="MAKRDFLTLLDCSGDELSALVARGRELKQNLARRDRPLLGHTAV
                     LILALNSTRTRVGFETGMNQLGGHTVVLNPEDTQLGRGEPIEDMARVLSEMVDVIIIR
                     TLEQTDIERFARAASVPVINAMSAKYHPCQLLADVQTFEELRGPIAGRTVAFVGDGHN
                     MCHSYINAARQWGFNLKIACPEGYSPDPSVLGSTNRASLVESAREAVEGADLVVTDVW
                     SSMGQEEEAARRREQFAPFQVNETLLDHAPDHTVFMHCLPAHRGEEISDKLLDDPRSA
                     VWQEAGNRLHSQKALLEFLILGEFPGSSGG"
     gene            complement(12621..13781)
                     /locus_tag="AC16QF_11375"
     CDS             complement(12621..13781)
                     /locus_tag="AC16QF_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HIR6449202.1"
                     /translation="MTHLMNNYARLPVAFARGSGAYLEDTSGKQYLDALSGIAVCGLG
                     HAHPRVTRAIAEQAETLLHTSNLYEVPLQERLADRLCEISGMDRVFFGNSGAEANEAA
                     IKIARLYGHKRGEKRPTIVVVDNSFHGRTMATLTATGNRKVQAGFEPLLAGFVRAPFD
                     DLDSVREIAGNNGDVVAVMVEPILGEGGIILPNDDYLPGLREICDQHEWLLILDEVQT
                     GNGRTGRYFAYQHADVLPDVVTTAKGLGNGVPIGACLARGQAAELLVPGTHGSTFGGN
                     PLVCAAAHAVLDALTEDGLIERAASLGERIAAGLKQSLAGNNRVVDIRGRGLMIAVQL
                     NEPCGRLVKAGLEAGILINVTRDDTVRLLPPLTLSDAEADQLVELVSGLVSA"
     gene            complement(13891..15069)
                     /locus_tag="AC16QF_11380"
     CDS             complement(13891..15069)
                     /locus_tag="AC16QF_11380"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIR6449203.1"
                     /translation="MPTETGTSEFDVEIDRTNTSSSKWDKYAGRDVLPFWVADMDFAM
                     PSFIRAAIAERLAHPVLGYTRTPDAAVQAFQNWLERCYGWEVPEEWLVWIPGVVTGFN
                     LAARAVAEPGGSVLIPTPVYYPFLSTPRHAGQDSIEVPLVRDGERWAMDFDAMSAACR
                     NGTRLFMLSNPQNPTGRAYTEEELSELAAFCLRHDLYLCSDEIHASIILAPEARHIPI
                     AAIAPEIAERTISLYAATKTYNIPGLGCAVAVIADARLRRDFRRAMGGLVPGIGPLAY
                     AASTAAFADASDWTTRLIDYLRGNHQRVREVVGERMTPVEATYLAWIDLTDLGFADPA
                     ARLEAFGIGLSEGADFGGAGYVRFNFACPRSLLDRGLARFRDALARLDAAQRAGASPD
                     "
     gene            15196..15522
                     /gene="grxD"
                     /locus_tag="AC16QF_11385"
     CDS             15196..15522
                     /gene="grxD"
                     /locus_tag="AC16QF_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HIR6449204.1"
                     /translation="MSEEVIATIEEQIATHPILLYMKGSPGAPQCGFSAQTVQCLVAC
                     GERFAYVDVLDNPEIRAALPGYANWPTFPQLWVRGELVGGCDIITEMFQAGELEPLIK
                     EAAETE"
     gene            complement(15530..>15931)
                     /locus_tag="AC16QF_11390"
     CDS             complement(15530..>15931)
                     /locus_tag="AC16QF_11390"
                     /inference="COORDINATES: protein motif:HMM:NF013122.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HIR6449205.1"
                     /translation="ASLKVTGIDLKDPNRQAAPENDALRGLFREVRRAVREAGCTRAI
                     LVAHNAAFDQQFLNNALARQQIKRSPFHPFSFIDTASLAAVTYGHTVLSEACARAHIE
                     FEPARAHNALYDAERTAELFCAIVNAWGRPR"
BASE COUNT         2739 a         4951 c         5309 g         2932 t
ORIGIN      
        1 agcgtcgtga tgaccgaaag accgggaccc acgatcccgc ttcgcgtcaa cgttcgctga
       61 gggcggcggc gccctcagct gccctcaatc ccagctgagc gccccgccag tctggtactc
      121 ggtgacccgg gtctcgaaga agtttttctc cttcttgaga tccatgatct cggacatcca
      181 cgggaacggg ttcttcgctc ccgcaaactg ctcggcaagc ccgatctgcg ccagacgccg
      241 gtttgcgatg aactggaggt actcggccat catgttcgcg ttcatgccga gtattcctct
      301 tggcatggtg tccttggcgt actcgatttc cagctgggta ccctcgagga tcatctgccg
      361 ggcgagagtc tgcaattcct cgtcccacaa ccccgggttc tcgagcttga tctgattgat
      421 cacatcgatg ccgaagttca cgtgcatgga ttcgtcccgc agtatgtact gaaactgctc
      481 ggaggtgccc gtcatcttgt tgaggcgtcc catggacaga atctgagtga acccgcagta
      541 gaagaagatc ccctccagca cgcagtaata ggcaatcaga ttcttcagca gcgccttgtc
      601 cgcctgggga gtgcccgtct ggaacgtcgg gtccgagatg tcccgggtgt acttcaacgc
      661 ccagccggcc ttgcgggcca cagcaggaac ctcgtggtac atgttgaaga tctcgccttc
      721 gtccatcccc agcgattcga tgcagtactg gtacgcgtgg gtgtggatgg cctcttcgaa
      781 tgcctgtcgc aggagatact gtcgacactc cggattggtg atcaaccggt agagcgcaag
      841 gacgaggttg ttggcgacca gggagtcggc ggtcgagaag aatccgaggc tgcgcttgac
      901 cacgagccgc tcgtcctggg tgagcccgga ctccgacttc cacagagcga tgtccgcggt
      961 catgttgatt tcctgcggca tccagtggtt cgcgcagccg tccagatact tctgccaggc
     1021 ccagtcgtac ttgaacggaa caagctggtt gagatccgcg cggcagttga tcatccgttt
     1081 ctgatccacg gtgacccgct gatagttctc ctcaatgcca tcctcggcga gcccctccag
     1141 gcccatctcg ccaatggcgg cttccgcgcg ctgcaacacc tcatcattac tcggcgcttc
     1201 ggacaagggc gcggccggct cgctgaccgt gatgggcggc aactcctcga cgggcctcgg
     1261 ggcaggcgca gctgcgcccg cagctgcctc cgcgacagcg cccgcgcccg cgacagcctc
     1321 gcctgggggc gggaaggatg cggccgcgtc ttccttcgac tcgagcggag cctcggcggg
     1381 cggttgctgc atcgctgtct ccgcgttcgc gtgttcttct tcgaagtcgt tccagctcag
     1441 cattttcgag tctcctttac tgacaggctt cacagtccgg gtcgtctatg gagcaggcca
     1501 gcgggacctg ggcaggccct ccggcagtca cgctgtccat gtcgagcggt gtcggcgctc
     1561 gagcggcggt ggtcgggtcg gcctgggtgg ccgacacggc gttgagcctg ccgcggtcga
     1621 gggtcgattt ctccgtgctg gtggcgccca gggagcggag gtaataggtg gtcttgagcc
     1681 ctctgagcca cgccattcgg taggtcacgt ccagcttccg gccggacgct ccggcgatgt
     1741 agaggttgag tgactgagcc tggtcgatcc acttctgccg gcggctcgcg gcgtccacca
     1801 gccacctcgg ttccacctcg aaggccgtgg cgtagagctg ctttagctcg tcgggcaccc
     1861 ggtcgatgga cagcacgctg ccgtcgtagt acttgagatc gttgaccatc accttgtccc
     1921 acaatccgcg gtctttcagg tcgtggacca tgtacgggtt gaccacggtg aactcgcccg
     1981 acaggttcga tttcacgtac aggttctgat acgtgggctc gatggactgg gagacgcccg
     2041 tgatgttggc gatggttgcc gtgggggcaa tggccatgac gttcgagttg cgcatgccgt
     2101 tgatctgcac gcggccgcgc agcttgtccc agttcatggt ggcgtcgaaa ttggcatcca
     2161 ggtactgggc gccacgttcc tgatgaagtt gcttcagcga atcgatgggc aggatgccgc
     2221 ggctccacag cgagccggag aagctcgcgt aggcaccgcg ctccttggcc agcttgcagg
     2281 aagcgtcaat ggcgtaatag ctgacggctt ccatcgaacg gtcggcgaag gtgatggcat
     2341 cttcgctggc gtaaggtata cgcagtttgt acagagcatc ctggaaaccc atgatgccga
     2401 gcccgatggg gcgatgccgc atgttggagt tccgcgcctg gggcaccgag tagtagttga
     2461 tgtcgatcac gttgtcgagc atgcgcaccg cggtgcgaac ggtctgcttg agcttgcgcg
     2521 tgtcgagctc cccgtcgcgg acgtgctgca ccaggttcac cgagccgagg ttgcacaccg
     2581 cgatctcttc gccgttggtg ttcagggtga tttccgtgca gaggttggaa gagtgcacca
     2641 cgccaacgtg ggactgcggt gaacgcacgt tgcaggcgtc cttgaaagtg atccacggat
     2701 ggcccgtctc gaacagcatc gagagcatct tgcgccacaa ttccggagcc ttgacccgct
     2761 tgaacagctt gagctccccg gtgcgcgtca tctcctcgta ttgctcgtag cgcttggcaa
     2821 acgcctgccc gtaaaggtcg tgaaggtccc tcacgtcgct gggcgagaac agcgtccagt
     2881 cctcaccttc ggtcacgcgc tgcatgaaca gatccggaat ccagttggcc gtgttcatgt
     2941 cgtgggtgcg gcgccggtcg tcgcccgtgt tcttgcgcag ctcgaggaac tcctcgatgt
     3001 ccaggtgcca ggtctccaga taggaacata ccgcgccctt gcgcttgccc ccctggttca
     3061 cggccaccgc cgtgtcattg accaccttta ggaaaggcac gacgccctgg gacttgccgt
     3121 tggttccctt gatgtaggag cccagcgccc gcaccggagt ccagtcgttg ccgagaccgc
     3181 ccgcccactt cgacagcatg gcgttgtccc ggatcgctcc gtagatgcct tccagatcgt
     3241 ccggcaccgt cgtgaggaag cacgacgaca gctgcggccg gagcgttccg gaattgaaca
     3301 gcgttggcgt ggagctcatg tagtcgaacg acgacaacag gtcgtagaac tcgatggctc
     3361 gggcgttgat gtcgtcttcc tgcacggcga gccccatggc gacgcgcatg aagaaaacct
     3421 gaggcagctc gaaccgcacg tcgttcgagt ggatgaagta gcggtcgtaa agcgtctgca
     3481 ggcccaggta agtgaacttg aggtcccgtt ccgacttgat ggctccgccg agagccgcaa
     3541 ggtcgaatgt cgccagcacc ggatccagga gctgcagctc gatgcccttc tcgatgaagg
     3601 cggccagcgc cgcgggatag tagccgccga cctcggattg ggtcgccctg tgctcgcctt
     3661 cggcattgac ttcgagaaaa cggaacgcct cggtcctcag ctcgtcgagc agcaggcgag
     3721 cggtcacgta ggtgtagttc ggctcttcct cgaccagcgt tcgggccgcg atctgcaggg
     3781 ccgtcgcgac gtccttggcc gagatgccgt cgtacatgtt ggacatggcc tcgtcgatga
     3841 tccgatcggc ctcgacgtcg ctgagcgaga cacaggcttc gcccaccagc ttgcgaattc
     3901 tatccaggtc gagcggctcg cggctgccgt cttcctgcac cacgttgagg gcgttgctgg
     3961 ctggttgcac cacgtctgcg gccctggcac gcgcgcgctc ctcgcggtag agaacgtaat
     4021 cgcgcgccac cttgtgctca ccggagcgca tgaggaccag ctccacctga tcctgaatgt
     4081 cttcgatgtg gatggtcccc ccggacggga accggcgctt gaacgtggcc gagacctgca
     4141 aggtcagctc ggcaacgagc tctctgattc gcggtgaggc ggcggcggtg ccgccctcga
     4201 cggccaggaa tgctttcgtg agagccacgg cgattttgtc gtcgttgtat ggcacgaccg
     4261 cgccgttgcg cttgatgacc ttgatctccc ccggggccgt ggcggcgatg ctcgggggca
     4321 gcggctgggc cgttgccatg gcggtcgccg tcgcctgcgg atcggacggg gtggcagtgc
     4381 cggaaatggg ttggttgtcg ctttgcattg ctagttgtgc gctcctgctc gctgtttcaa
     4441 aaacgcctga accgttgaac tcgctcgcac cgggtcgacc gcaggtttcc acggtcgcag
     4501 tgccccggtc gtgccgcgag agggacgtgc ggcggcgacc gtggaaacac aatacctagt
     4561 attagcggct ccggcagaaa taggtcccct ccgaacagat ttctgtatgg ctcgcgtggt
     4621 caggcgcttc gctgacttgc tgctgcggcg actggctccc ctgcgactgg cagtcaataa
     4681 attgaccgag tcaatgctca taaccgcaac atgttgggtt tttcagccga ttcgaaccca
     4741 agaccacaca agataattcg attgcaggag ggttgcaagg aattagctgg ggatacgttg
     4801 tggatatgct gtgggccacc cggacggcgg acattttctg ccgtcgcgcc tctcgccgaa
     4861 ccggggcgac cactatatat tgtggccggt tttcgtggta ccggactctc ctcatgagcc
     4921 atctactcgc cctcgaccag ggaacctcca gctccaaagc catcattttc aacctcaccg
     4981 gtcgggtggt gggcgtaggc cagcacgagt tcgacaacac cttcccggcg gacggctggg
     5041 tggaacagga cccggaagtt ctgtggcgga ccacgctgct ggcggggcgc gaagcgctgg
     5101 ccagcgcggg tctgaaggga gaggacatcg ccgccatcgg catcaccaac cagcgggaaa
     5161 ccaccctggt ctgggatcgc cagaccggcc gctgcccgca caacgccatc gtctggcagg
     5221 accgccgcac cgccgatcgc tgcgagctga tgcgcctcga tggcctggcg gaagcggtgg
     5281 ccgagaccac gggcctgctc atcgaccctt atttttccgc aacgaagctc gcctggattc
     5341 tcgacaacgt ttccggcgcg cgcgagcagg ccgagtcggg cgcgctggcg ttcggcacgg
     5401 tcgatacgtt cctcgtctgg cggcttacca agggccggca ccacgccacc gatgcctcca
     5461 acgcgtcacg caccatgctg ttcgacattg ccgggcagac gtggagcgag cggctgctca
     5521 actacttcaa catccccgcg gcgatgctgc ctgcggtcag ggacagcgcg gatgacttcg
     5581 gcgtggccga cccggagtgg ttcggcgcgc cgataccgat ctgcgggatt gccggcgacc
     5641 agcaggcggc gttgatcggg cagggctgct tcaaccccgg aatggcgaag agcacctacg
     5701 ggacgggctg ctttgccatg atcaacaccg gggcagagca gctgcgctcg cagcagcagc
     5761 tgctcactac cgtggcctat cgcctcgacg gggagatcac tttcgcgctc gagggcagca
     5821 ttttctcggc aggggtcgcg gtcaagtggt tgagagacca gctcgggctg gttgtcgatg
     5881 cggcggacac ggaggccgcg gccatccgcg cccagggaga taccgcaggc gtctttctgg
     5941 tgccggcgtt caccggcctc ggagcacctc attgggcgcc cgaagcccgg ggtctggtgt
     6001 ccgggctcac cctggcgacc acccgcgacc agctgatcac cgccacgctg caggccgtgg
     6061 cctttcagag ccacgagctc atcgccgcca tggcctcgga tggagccgtc gtcgacaacc
     6121 tccgcgtgga cggtggcatg gtggtcaacg actggctgtg ccagtgcctt gccgatgtgg
     6181 tgaacctccc ggtggagcgt cccgagatca ccgagaccac ggcgctggga gccgccatcc
     6241 tggcggcgct tggcgctggg ttggtcgagg atctggactc ggcggcaagg ctttggaacc
     6301 tcgagcggga gtttctgcct gccatggacc cggcacgccg ggagagcctg ctcaagggct
     6361 ggcgcgccgc cgtggccagg acgctcctct gagaaagttt gtcccgcgtc acgttttagc
     6421 caaaaaggcg ggcctggcgc gcgattcccg ccgccaaggc ggtaccacgt cctgtattgg
     6481 cttcgtgcag attccctaga attcagctca gctaggagat ttgcagggaa tagcattgct
     6541 ttccgcgctt caaatcgccg gaacgaagct ggtgcgaata gtggcatctc tcgtcgccat
     6601 gacgggatat ctgcttttct gcgtcatgct gtttcgtttc ggttatctgg ataccggtcc
     6661 gccggtgttc tacacgttgc tgaccctctg gagcgtcgtc ctggcggctc tggtggtttt
     6721 cgacgtgccc agcgcacggt tcgacagggg atacctgacg ttcttcaaag cgctgtggtg
     6781 caacgtgggt gtggttgcga ccgcccttgt cgttccggct tccctgcggc ccctgatgct
     6841 ggtcgtgccg ctgttcggcg tgctctacac ggcgctgcat ctcgagcgca aacacgtcct
     6901 cgcggtgggg acggtgacct ggttcaccta tgtcctcggc gcgctgatcc agaacgacat
     6961 gcgcttcatc gacgagacct cgcccggcct ctccacggag ggcatggtgg tggtcgcctt
     7021 cgccctcatg ctcatcgtga tgacggtcat gggtggtgag gtgacggctc tgcgcaatgc
     7081 gttcgcccac cgccgcgagc ggctcaagca ggccatggaa aggctatctg agctggccat
     7141 gcgcgacgag ctcaccggct tgtacaaccg gcgttacatc atggatgcgc tggcccagca
     7201 gaaggccgtt gccgaccgcg gccgcctggg ctttgccgtg ctctactgcg acctggatca
     7261 cttcaagcgg atcaatgacc ggttcggcca ccaaagcgga gaccgggcgc tgtgtgattt
     7321 cggcaagctg gcatccagcg tggtccgcag catcgactac gttgcgagga tcggcggcga
     7381 agaatttctg ctcgtgctcg tcggtgccga catcaacgac gccacgatcg tggccgagcg
     7441 gctgtgtaaa cgcacgcgca acctgccgat ctccgctcag ggcggcgaat accacctgac
     7501 cgtatccatc ggcgtgtcga gctttcgctc gggtgagcgt gtggaggatg tgatccagcg
     7561 cgcagatctg gccgtctacc gggcgaaatc gggcggccgt gaccaaatca gcgtgttggt
     7621 gtgagcggat gagaacaaca gtaactaggc tagcaaagag gtagggcctc ctcgcagaag
     7681 gacgctgcag ttttggaaaa tcggcgacgc tatcccaggt atccgatctc gctttctgct
     7741 ctgatgcatc cggctcaggg cagatcgtgg ctgtgcacga tcagcaactt ctgtcgtgac
     7801 gggatgcttc tggtaggcaa cggcgggccc ggatcgctcg acgccaccgg ggcggacgcc
     7861 aagccgggcg acaccgtggc gctgcacttc tcggtggctt tgcccggggg tcagaagcac
     7921 tttcgcaccc aggctcgcgt agcccgcata ctgggttcgg gcgagggcat gggcgttcac
     7981 ttccccggcg gtctgcccga cgatgcctac gataccctga tggcattcga ggccgcaggc
     8041 aacccgtccg gggagaacag ccagcccctg gacgaagacc ctccggacga tgtggagagc
     8101 ccgcaagccc cggtcgcccc ccagcgcatc tcacacgagg atgccgatga aatacggcgc
     8161 aagatgcgtc ggatcaccca gccgaccctc gatgcgctct gtcagaagtg cttcgagctg
     8221 atcgaggaag atctgcgcga gaagacgctg gcgagcctcg gcgacggcct gggcgagatc
     8281 tacgattcgg gccgggaatt gatcgagaag taccgcaacc agatccgcaa cgacttcatt
     8341 ggcggcgtgc ttcggcagat atccaacgtc gcgcagctcg aggacattgc ccccctgcgc
     8401 tcgcgcaggg aggaggcgat ccggcgtgag cacgagcagg ccggaagtga caagttcgac
     8461 ggttggctgg acgtcgccga ggtcgttgcc aaagccgaaa gccgctaccg ggacgacctg
     8521 cgcgatcttc gcgcacgtct cgggctcgtc gagccgccct ggcggcacaa ggaagtggtg
     8581 cccgtgggac ctgctgccat tacctgggcg ttcgacggcg cgctgaagga tctggagctg
     8641 accccgcgga tccgaaccca cgtcttcgaa ggctttcagg cggcgctgct tccggcgctc
     8701 aacgacttct atcacggcct tggcgagctg ctttccgaat gcgagatctt tcctacggaa
     8761 gaagccctcg aagcgatcag cgctctccag gagccggccc gcgacgacga cctgacgctt
     8821 cacgaatacg taccggggtc cgcccggcac ggcgaggctt ccggcgacga gccggacgcc
     8881 ggggaggaag ccgcggtggg cgtgaagctg gctacgcacg atgagccgga agcggagcag
     8941 gttggccccg cgccgcgcga ggtctacgcc actgcccgca gcattgtgaa actcgagcgc
     9001 gtcgccaggc agcagcttga aggcgagggg ggcgaaccgc ggcaggcagc cgggggcggc
     9061 gcagccaacc cgttcggcag cgaggagatc ctccgggtgc tgggcgcgct ggggccggga
     9121 ggtcaaggcg accgggccac cgtttgtcag cgcctggtgc aggagctgga tgcggccgac
     9181 ggcgacagaa gcctcggcga gagtgacctg gaccgttttc gggtgctcga aagcctcatc
     9241 gactccatct gtgaggaccg gttcttgagc caaggcagct tgaactgggt ccggcgcctg
     9301 gaagtgactc tctacaagct ggcggtacac aaccctggtt tccctggagg ggatcccgac
     9361 cggccccacc cggccgagat catgctcaat cagcttgccc gtctcagcgg catcgcggat
     9421 tcccgggaag cgaaggcgcg tgagctgagc ggtcgcgtgg atgcgctcgt ggagcgtgtc
     9481 gcagacggct actccgggga cggcggcatc tttgaagagg cggtcgccga gctgaatccg
     9541 ctggtggatg agcagatccg gaactaccgt gggaacatgg agcgcatcgt gcgcgccagc
     9601 gagggacagc aacgcctgac ccgggctcga aaagcggtcg tgaatgcctt ggctccgaag
     9661 atggcgggca aagaggtccc ggagccgata ctcaagctgc tgaatcccgg gtggcgcaac
     9721 ctgatggtac atacctgcct gcgaatgggt gcgggaagcg ctcagtggga ggagcgggtc
     9781 gccgtcatcg aacggctgtg ggccgagttc gtcgccgagc ccgagcaggg cggcagggac
     9841 gcctccgacc tcaagcagct gctgcggcag gttgccgacg gcctgatcag catctccttc
     9901 gatccgggtc tgcgcaagcc tctgcttctc gacatcgcga aggtgttgcg ggcgaaggcc
     9961 gacggcaagc cggcacccgt caagaccgtc agcgttgcgg cggacggcgt ggcggacgcg
    10021 ctgggcattg ccaggcttct tccggagtcg gatgcggaac tcgaagccgc gcaacaggaa
    10081 atgcagcgga gctgggccaa gtcgctgagc cgagcccgga ccctgcaggc gggggactgg
    10141 atcgccacgg atgacgccca gggacgccca ttgattctgg tcgtggcctt cgtcggcgat
    10201 gagtcgagcg gctttgttct ggccaaccgc aacgggagga aggagcggga actcacgctg
    10261 accgagatgg cggacaacct gcacgaagct cggatgaccc tgctgagcga cttcgacatg
    10321 ccgctcgtgg agcgcgcggc ggaacggatg ctggagaccg tgcacggcag gcttgccgac
    10381 cagtccggtc gggatgcgct gacgcagctc gtcaaccggc gggagttcga gcggcggctc
    10441 gacaaggctc tgaagaggtc gagggcggat ggcgtggatc acgccctgat gctcatcgac
    10501 ttggaaggcg tcgaggcggt gcggtcggat acgggagatg cgggcgccga cgagctgcta
    10561 aagggcgccg cggacgttct gaccagggcg ctcattggtc ggccggcgga catcggccgc
    10621 gtcgacgcgg accgcttcgg cattcttctc gaacgcacga gcgggccggc cgcgatggaa
    10681 attgccgagc agatcctcaa ggcgctcggc gacgatccct tgcgcgtgag cagcggccag
    10741 caggcgcttt ccgccagcat cgggctggtg agtatggata cgcagtccga gtcggcaaat
    10801 gcggtcatgc actatgccga aagggcatgc cgggcggcca ggctgtcggg tcgcaatcag
    10861 atcaaggagt tcgagatcgg ggagattgcg cagctgcggc gccacgggga agccgcgtgg
    10921 gccttcaggc tggatgaagc catggccggt gagggcctta tcgtgagttt ccgccagatc
    10981 gccccgatga gcggcgcgcg caacggcgtc gatacgcact acgaagtgca gctgaccatg
    11041 cgcgacgagg aaggtgggat catggccccc gagaaattcc aggatgcggc ggaaagccac
    11101 gaacgcatgc ccgccctgga ccgctggact gtggcctggt gtctcgactg gatggcggct
    11161 cacaaggaca gcctggatca tttcggcggt ttcgcgatcc ggctgtcgga tcgctcgctg
    11221 aacgagccga cgttctcgga cttcgttctg gagcggttct ccgcctgcca ggtcccgacc
    11281 agcaagatct gcttcgagat caccgagtcg gtggcggttg ccaacatcga gaacgcggtg
    11341 gagttcatga accgcatgaa gatcatcggc tgccgtttcg ccctggagga cttcgggtcg
    11401 ggccgcgcgt cctatgccta cctgcgccgc ctgcccgtag actacgtgaa gatcgacgac
    11461 gtgttcgtcc gggagatcgc ggccaatccc ggcgactacg ccgtggttcg ctcgatcaac
    11521 gagatcggtc actacatggg caagaagacc atcgcggagt tcgtcgagga cgaggaggtc
    11581 cgcgcccggc tggccgagat cggggtggac tacctgcagg gcatggatgc ccagagcgcg
    11641 atcccgctgc ggtaatcgcc cagccgtcct ccggcttgaa atcaaccgcc ggacgagcct
    11701 ggaaattccc ccagaatcag aaactcgagc agcgccttct ggctgtgcaa tctgttgcct
    11761 gcttcctgcc agaccgcgga gcggggatcg tccaagagct tgtcgctgat ctcttcgccc
    11821 cgatgggcgg gcaggcagtg catgaagacg gtgtggtccg gagcatggtc cagcagcgtc
    11881 tcgttgacct ggaacggcgc gaactgctcg cggcgccggg cggcttcctc ctcctggccc
    11941 attgaagacc agacgtcggt gaccaccagg tcggctccct ccacggcctc ccgggcggat
    12001 tcaaccaggc ttgcccggtt tgtggagccg agcaccgagg ggtcgggtga gtagccttcg
    12061 ggacaggcta tcttcaggtt gaagccccat tgccgggccg cgttgatgta ggagtggcac
    12121 atgttgtggc catcgcccac gaacgccacc gtgcgccccg cgatgggacc gcgcagctcc
    12181 tcgaaggtct gaacatcggc gagcagctga cagggatgat acttcgcgct catggcgttg
    12241 atcaccggga cgctggccgc gcgggcgaag cgttcgatgt ccgtctgctc gagggttcga
    12301 atgatgatca cgtccaccat ctcggaaagc acccgagcca tgtcctcgat aggctcgccg
    12361 cggccgagct gggtgtcctc tgggttgaga acgacggtgt ggccgccgag ctgattcatg
    12421 ccggtctcga accccacccg ggtgcgggtc gagttcaggg cgaggatgag caccgccgtg
    12481 tgcccgagta acggtcggtc acgtcgggcg aggttttgct tgagttcccg gcctcgcgcc
    12541 acgagcgcgc tcagctcgtc gccggaacag tcgagaaggg tgaggaagtc gcgcttggcc
    12601 atggtggcag gttccgcggg tcaggcggag acgagaccgc tcaccagctc taccagctgg
    12661 tcggcctcgg cgtcgctcaa agtcagcggc ggaagcagcc tgacggtgtc gtctcgggtc
    12721 acgttgatca gaatcccggc ctccaggccc gccttcacca gcctgccgca tggctcgttg
    12781 agctgcaccg cgatcatcag gccgcgaccc cggatgtcga cgacccggtt gttgccggcc
    12841 agggattgct tcaggccggc tgcgattctt tcgccgaggc tggcggcgcg ctcgatcagc
    12901 ccgtcttccg tcagcgcgtc cagcaccgcg tgggccgcgg cgcagaccag cgggttgccg
    12961 ccgaacgtgg agccgtgggt gcccgggacc agcagttccg ccgcctgacc acgggccagg
    13021 caggcaccga tgggcacgcc gttgccgaga cccttggccg tcgtcaccac gtccgggagc
    13081 acgtcggcgt gctggtaggc aaagtagcgg ccggtgcggc cgttgcccgt ctggacctcg
    13141 tccaggatga gcagccactc atgctggtcg cagatctctc gcagcccggg aagatagtcg
    13201 tcgttcggca ggatgatgcc cccctcgccg agaatgggct ccaccatcac ggcgaccacg
    13261 tcgccgttgt tgcccgcgat ctcgcgaacc gagtccaggt cgtcgaaagg agcgcggacg
    13321 aatccggcca gcaggggctc gaagcccgcc tgcaccttgc ggtttcccgt cgccgtcagc
    13381 gttgccatgg tgcggccgtg aaagctgttg tccacgacca cgatggtggg tcgcttttct
    13441 ccccgcttgt ggccgtagag ccgggcgatc ttgatggccg cctcgtttgc ctcggcgccg
    13501 gaattgccga agaagacccg gtccatgcct gagatctcgc agagccgatc ggccagccgc
    13561 tcctgcaggg gaacctcgta gaggttggaa gtgtggagaa gcgtttccgc ctgctcggcg
    13621 atggcgcgcg tgacccgggg atgggcgtgc cccaggccgc agacggcgat gccgctgaga
    13681 gcgtccagat actgctttcc ggacgtgtcc tcgagatacg cgccgctacc ccgggcgaac
    13741 gccacgggta ggcgggcata gttgttcatg aggtgcgtca tgtgctgcct gcggatcgtc
    13801 gtagggaaga ttttcgcctc cccaaaacca ttccaggcag ctgttgctgc ccggaaaaac
    13861 gctgaatcgt actgcagggg agtggtcagt tcaatcgggc gacgcacccg ccctctgcgc
    13921 tgcgtccaac cgggcgagcg catctcgaaa gcgtgccagg ccgcggtcga gcagggatcg
    13981 agggcaggcg aagttgaaac gcacgtaccc ggccccgccg aagtccgccc cttcggaaag
    14041 accaatgccg aatgcttcca ggcgagcggc gggatcggca aagccgagat cggtgagatc
    14101 gatccaggcg agataggtgg cctcgacggg cgtcattcgc tcgccgacca cctcgcgcac
    14161 gcgctggtga ttgccgcgca ggtagtcgat caggcgcgtc gtccaatcgc tggcgtctgc
    14221 aaaggcagcg gttgaagccg cgtaggccag cggtcctata cccgggacga gcccccccat
    14281 ggcccgacgg aagtcgcgtc gcaggcgcgc atcggcgatc accgccaccg cgcagccgag
    14341 gccgggaatg ttgtaggtct tggtggcggc gtaaaggctg atggtgcgct cggcgatttc
    14401 cggagcgatg gcggcaatgg gaatgtgacg agcctcgggc gccagaatga tgcttgcgtg
    14461 aatctcgtcc gagcataggt agaggtcgtg gcgcaggcag aacgccgcga gctcggacag
    14521 ctcctcctcg gtatacgcgc ggcccgtcgg gttctgcggg ttgctcagca tgaacagccg
    14581 tgtcccgttg cggcaagcgg cgctcatggc gtcgaagtcc atggcccatc gctcgccgtc
    14641 tctcacgagc gggacctcga tggagtcctg gcctgcatgg cggggggtcg acagaaacgg
    14701 gtagtagacg ggtgtgggaa tgagaacgga gcccccgggc tcggccaccg cgcgcgcggc
    14761 caggttgaat cctgtcacca ccccgggtat ccagaccagc cactcctcgg gcacttccca
    14821 gccatagcag cgctcgagcc agttctggaa cgcctgcacc gcagcgtcag gagtgcgcgt
    14881 gtatcccagc acgggatgcg cgaggcgctc cgcgatggcc gcgcggatga atgacggcat
    14941 ggcgaaatcc atgtccgcga cccagaaagg gagcacgtcg cggcctgcgt acttgtccca
    15001 tttggagctc gacgtgttgg tgcgatcgat ctcgacatcg aactcgctcg ttcccgtttc
    15061 ggtcggcatg ttccctccgt gcaccgatcc gcgccttgat ggactgcgct ccggctccct
    15121 gttgcgcggc ctgatcggag ggtagaatgt cggcattcca tgccaagcgc aattcgcgtc
    15181 gagggttgac gagcaatgag cgaagaagtg atcgccacca tagaggagca gatcgcgaca
    15241 catccgattc tgctttacat gaagggcagc ccgggcgcac cgcagtgcgg attttctgcc
    15301 cagacggttc agtgcctggt cgcctgcgga gagcgatttg cctacgtgga cgtcctcgac
    15361 aacccggaaa tcagagccgc gctgccgggc tacgcgaact ggccgacctt tccccagctc
    15421 tgggtgcgcg gcgagctcgt cggaggctgc gacatcatca ccgagatgtt ccaggcgggt
    15481 gagctcgagc ctctgatcaa agaagccgcc gagacggagt gacgatcgtt caacgcggac
    15541 gcccccaggc gttcacgatg gcgcagaaaa gctcggccgt tcgctcggcg tcgtagagcg
    15601 cgttgtgtgc ccgggccggc tcgaactcga tgtgcgcacg cgcgcaggct tcgctgagca
    15661 ccgtgtgacc gtaagtcacc gcggccagag acgcggtgtc gatgaagctg aacggatgga
    15721 aagggctgcg cttgatctgc tggcgcgcaa gggcattgtt gagaaactgc tgatcgaacg
    15781 cggcgttgtg cgcgaccaga atggcacgcg tgcagccggc ttctcgcacg gcccggcgca
    15841 cttcccggaa aagacccctc aacgcatcgt tctccggtgc cgcctgccgg ttgggatcct
    15901 tcaggtcgat gccggtcacc ttcaacgaag c
//
LOCUS       DBNHLM010000247         5826 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_513784, whole genome shotgun sequence.
ACCESSION   DBNHLM010000247 DBNHLM010000000
VERSION     DBNHLM010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_513784"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..1630
                     /locus_tag="AC16QF_11395"
     CDS             236..1630
                     /locus_tag="AC16QF_11395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6449206.1"
                     /translation="MPAASAGPLRFNEKLGYAFGDTASNFFFQTFNIFLLYYYTDVFG
                     ISAAAVGTLFFVSKLWDAVNDPLMGALADRTNTRHGKYRPYLLWMALPYGLMGYLMFA
                     NPDFDDTGKLVYAWVTYSLMMMLYTAINVPYSALMGVMTPSTTERTLLASYRFVGAFG
                     GGLLISMLVRPMVHYFGAEDEAAGFQVTMALFAVISVVMFWYTFGATRERVRSLPETD
                     VSFGNDVKLLLSNRPWIVLFFAGIFTLSSIAIKNAVTIHYFKYYVGDDSSPILLFFDI
                     TTLFMTIGMLALIAGVACTKWLTARFEKKTLLIALSLGNAVTMVAFFFVPPDQLALML
                     ALNITGSLMAGPTAPLVWAMYADVADYGEWRFERRTTGLVFSASMFAQKFGLTIGAGL
                     AGWMLALFGYVANVTQSDDSLMGIRLMFTLFPAAVSLLNVAVIAFYTLSDAEVESIGR
                     ELEDRKAQAGPAPI"
     gene            1695..3758
                     /locus_tag="AC16QF_11400"
     CDS             1695..3758
                     /locus_tag="AC16QF_11400"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6449207.1"
                     /translation="MAEVSEQMPQLFISYSRDDGRAVYPDADWLGGLGYAVHIDRHIS
                     PGEQWREAIANAIADSDLLIFYASPSSIESKFCSQELQFALSRDIPVITVMLEETPFP
                     PWMELALGNHQILQRFDVPQRAYRSLLESGIANQLDQIPPAAPPLTSPRGRWRGMWIG
                     AGAITALVFVLWRSFLPEPLAIAVAPFETDGSLRAAEMAGGVTAELSQGLTNLDLMPV
                     KSRLPDRRPAAGAFVGGSNDASHVIIGHVQLLDPGYRIRTSLIATEDDQEIWAQTFVY
                     VEDDPKEVQHQHVQNLLVALPDVLVGTIPALDRAAPSARAAAQQIYGEGARMLAQPGE
                     LQHVDTIRQLFEQALDNQPGFGLALLGLCDAHARTYQESRELDDLLHAETRCLQALAV
                     GSEPLKAHLALGRVFLARGEAQLARFHFQRASAVAPGLAEPLIGLADVADQNGDLEMA
                     RAYYEQALALPGTSWEAHLAYGNHLLYQGEFRAAAEQYEVVADLYPAAAIAYVNLGAA
                     WYSLGEFERASQAWRKSVELEPNPMSWGNLGAAQTYARNFSAAANAYREAAALAPSDH
                     RWLGHLGEILAYGPTGDAEEAQRILQQALDLALEELAFTREEQGPVQARISTYEALLG
                     RDADAMARARRVRAEHEDDMTVAYSVLQTYTLLNRATLAARERERLLGLGYPALLLER
                     DPWLN"
     gene            3808..4380
                     /locus_tag="AC16QF_11405"
     CDS             3808..4380
                     /locus_tag="AC16QF_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449208.1"
                     /translation="MNAQLSTTSIKRLSVKAVLACLACIGLAGCVSESTTNTWGTQLF
                     SVSPDNFSCSKAPDMRLKIEQTAGDASGILCVTGGGDVNCTNPAPNGPAVVLRNQLIQ
                     WELSSGSRTEPFYIIFDEDKSPAQKGKNRGANVVASENGKLCMRVNGRATASAQGEPY
                     RYSVYVKHCAGAPTCTPKVLDPIIYVRKVY"
     gene            complement(4417..4791)
                     /locus_tag="AC16QF_11410"
     CDS             complement(4417..4791)
                     /locus_tag="AC16QF_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449209.1"
                     /translation="MAEQQHQAGKLDVGALIHSSGGVLCLVLGGLMYLDGFAWAVHAF
                     SAMMLLTFVSALIISINTDSWVAGHYLAMSPVVGVVLGYADFDWGFTVAYLLIWAAFL
                     HFLWRGYQAVQAAKRESGTRRV"
     gene            4953..5801
                     /gene="yghU"
                     /locus_tag="AC16QF_11415"
     CDS             4953..5801
                     /gene="yghU"
                     /locus_tag="AC16QF_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020800808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HIR6449210.1"
                     /translation="MTKSSAYVPPKVWKWDTESGGRFANINRPTAGATHDKELPVGEH
                     PLQLYSLATPNGVKVTVMLEELLDLGKTDAEYDAWLINIGDGEQFGSGFVAINPNSKI
                     PALLDRSTETPTRVFESAAILLYLAEKFGAFLPEDLSSRGECFSWLFWQMGSAPYLGG
                     GFGHFYAYAPEKFEYPINRYTMEVKRQLDVLDQNLAQRRYLIGDEYTIADMATYPWYG
                     AVVLHNIYDAAEFLDVTSYEHVIRWAKEIEERPAVQRGRRVNKVWGSEETQMPERHSA
                     SDFGSV"
BASE COUNT         1117 a         1888 c         1767 g         1054 t
ORIGIN      
        1 gagcatgtca ccggaattcc agttgacgat gaccacgtca acgctccgat ctgttgcaaa
       61 gcaggtatcc aacgacaggc gcaaccccac aagactcagc tcattctagc gacacgcact
      121 cccgggcgcg caccctctcc cgaccttttc ctcagcaatc gccatggata aatgatagcg
      181 ttgtcatcct gatttgcgac ccgctcgtcg ccaggcgacc gggagagggc cagacatgcc
      241 agccgccagc gccggaccgc ttcgcttcaa cgaaaagctc ggctacgcgt tcggcgacac
      301 ggcctccaat ttcttcttcc agaccttcaa catctttctg ctctactact acacggacgt
      361 cttcggcatc tcggcggcgg cggtgggaac gctgtttttc gtctcgaagc tctgggatgc
      421 cgtgaacgat ccactcatgg gtgcgctggc agaccggacc aatacccggc acggaaaata
      481 tcgcccgtac ctgctctgga tggctctccc ctacggcctc atgggctatc tgatgttcgc
      541 gaacccggac ttcgacgaca ccggcaagct ggtctacgcc tgggtgacct attccctgat
      601 gatgatgttg tacacggcca tcaacgtccc ctactccgcg ctcatgggcg tgatgacccc
      661 cagcaccacc gagcgcacgc tgctggccag ctatcggttc gtgggtgcgt tcggtggcgg
      721 gctgctgatt tcgatgctcg tccgacccat ggtccactac ttcggcgcag aggacgaggc
      781 cgcggggttt caggtcacca tggcgctctt cgccgtgatc tcggtggtga tgttctggta
      841 cacgttcggc gctacccgcg agcgcgtgcg ttccctgccc gaaaccgacg tcagcttcgg
      901 caacgacgtg aagctcctgc tcagcaaccg accctggatc gtgctcttct tcgcagggat
      961 cttcacgctg tccagcatag ccatcaagaa cgccgtcacg attcactact tcaagtacta
     1021 cgtcggcgac gacagctccc ccatcctgct cttcttcgac atcaccacgc tcttcatgac
     1081 catcggcatg ctggcgctca tcgctggggt cgcctgcacc aagtggctga cggcccgttt
     1141 cgagaagaag accctgctga tcgctctgtc tcttggcaac gccgtgacca tggtcgcctt
     1201 cttcttcgtc ccgccggacc agctcgcgct gatgctcgcg ctcaacatca ccggttcgct
     1261 catggcggga cccacggccc cgctggtctg ggccatgtac gccgacgtgg ccgactacgg
     1321 cgagtggcgc tttgagcgac gcaccaccgg gctcgttttc tccgcttcca tgttcgcgca
     1381 gaaattcggc ctgaccatcg gcgccgggct ggcaggctgg atgctcgcgc ttttcggata
     1441 cgtagccaac gtcacccaga gcgacgattc gctgatgggt atccggctca tgttcaccct
     1501 gttccccgcg gcggtgagcc tgctgaacgt ggccgtgatc gccttctaca ccttgagcga
     1561 cgccgaggtg gaatccatcg gccgggagct cgaggaccgg aaggcacagg cggggcctgc
     1621 tccgatctga gcctcggtgg cagccatcgg cgcctctggc ataatcctga gttggggttt
     1681 ccggggcggg atccgtggca gaagtatcag aacagatgcc gcagctgttc atctcatact
     1741 ctagagatga cggcagagcc gtatatccag acgcggattg gcttggcggt ctcggatatg
     1801 ccgttcatat cgacagacac atcagtcccg gagagcaatg gcgcgaggcg atcgcgaacg
     1861 ccatcgcgga cagtgatctg ctgatctttt acgcctcccc gtcctccatc gagtccaaat
     1921 tctgctccca ggaattacag ttcgctctga gccgcgacat tcccgtcata acggtcatgc
     1981 tggaagaaac cccgtttcct ccatggatgg aactggcgct gggtaatcac cagattctgc
     2041 agcgcttcga cgtgccgcag cgtgcgtacc gatccctgct ggaatcaggg atcgcaaatc
     2101 agctcgatca gattccaccc gcggccccgc ctttgacgtc cccgcgcggt cgctggaggg
     2161 gaatgtggat cggcgccgga gcaatcaccg cgctcgtctt cgtcctgtgg cgaagcttcc
     2221 tgcccgagcc tctggctatc gccgtagcgc ccttcgaaac cgacgggagc cttcgagcgg
     2281 ctgagatggc ggggggcgtc acggcggaac tctcacaagg actcaccaac ctggatctga
     2341 tgccggtgaa gagccggctc ccagatcggc gccccgcagc aggcgcgttc gtcggcggca
     2401 gcaacgacgc aagtcatgta atcatcggac acgtccagct cctggacccc ggttaccgga
     2461 tccgcacatc tctcatcgca accgaggatg accaggagat ctgggctcag acgttcgtgt
     2521 acgtcgagga cgacccgaaa gaggttcaac atcagcatgt gcagaatctg ctggtggcct
     2581 tgccagacgt gcttgtcggc accattccag ccctggatcg ggccgcgccg agcgcgagag
     2641 cggcggcgca gcagatctac ggcgagggtg ctcggatgct ggctcagccc ggcgagctgc
     2701 agcacgtgga tacgatccga caactgttcg aacaggccct ggacaaccag cctggctttg
     2761 ggctcgcgct gttgggcttg tgcgacgctc acgcccgaac ctaccaggag agccgcgagc
     2821 tagacgacct gcttcacgcg gagacccgct gcctgcaggc gctggccgtg ggttccgaac
     2881 cgctgaaggc gcatctggcc ctaggccggg tctttctggc ccgaggcgag gcacagctcg
     2941 cccgattcca ctttcagcgt gccagcgcgg tggctcccgg ccttgcggaa ccactgatcg
     3001 gcttggcgga cgtggccgac cagaacggcg atctggagat ggctcgcgcc tactacgaac
     3061 aggccctggc gctcccggga acgagctggg aagcccacct cgcctacggc aatcatctgt
     3121 tgtaccaggg cgagtttcgg gcggccgccg agcagtacga ggtggtggcc gatctgtacc
     3181 ctgccgctgc aatcgcctat gtcaatctgg gcgcggcctg gtacagcctg ggcgagttcg
     3241 agcgggcgag ccaggcgtgg cgcaagagcg tcgagctcga gcccaacccc atgagctggg
     3301 gaaacctggg cgccgcacaa acttatgcgc gaaatttttc tgctgcggcg aacgcgtacc
     3361 gggaggccgc cgccctggca ccctccgatc accgctggct cggccatctg ggggaaatcc
     3421 tcgcctacgg cccaaccggt gatgccgagg aagcccagcg tatcctccag caggcgctag
     3481 atctcgccct ggaggaactg gctttcacca gggaggaaca gggcccagtg caagcccgaa
     3541 tcagcacgta cgaagccttg ctcgggcgtg acgcggatgc catggcccgg gcaagacggg
     3601 taagggctga acatgaagat gacatgacgg tcgcctacag cgtgctgcag acctacacgc
     3661 tgctgaaccg ggcaaccctg gccgctcgcg agcgcgagcg gctgctcggc ctcggttacc
     3721 cggcgctgct gctggaacgt gacccgtggc tcaactgacg caaccagggg cgcgcaccgc
     3781 gccagataac ctcaaatcgg aggacgtatg aacgcacaac tttcaaccac ttctatcaag
     3841 cggctgtcgg taaaggccgt ccttgcctgc ctggcatgca tcgggcttgc cggttgcgtc
     3901 tcggaaagca cgacgaacac ctggggtacg cagctcttct cggtcagccc ggacaatttc
     3961 agctgttcaa aggcccctga tatgaggctg aagatcgagc agaccgccgg ggacgcaagc
     4021 ggaatactgt gtgtcaccgg cggcggggac gtcaattgca cgaatccggc tccgaacggt
     4081 cccgcggtgg tgcttcgcaa tcagctcatt caatgggaat tgagcagcgg cagcaggacc
     4141 gagcccttct acatcatctt cgatgaggac aaatcacccg ctcagaaggg caagaaccga
     4201 ggcgccaacg tcgtcgcctc ggagaacggc aagctctgca tgcgcgtgaa cgggcgggcg
     4261 acggcaagcg cccagggcga accgtaccgc tacagcgtgt acgtcaagca ctgcgcaggc
     4321 gcgccaacgt gcacgcccaa ggtattggac cccatcatct acgtcaggaa ggtctattag
     4381 ccgctaagga ggcggccatg cctgaacggc cgcgctctac acgcggcgcg ttccggattc
     4441 ccttttcgcc gcctgaacgg cttgatatcc acgccagagg aagtgcagaa aggccgccca
     4501 gatgagcagg taggcgacgg tgaatcccca gtcgaagtct gcgtaaccta agaccacgcc
     4561 gaccactggc gacatggcca ggtagtgacc ggcgacccag ctgtcggtgt tgatggagat
     4621 gatcagcgcg ctgacgaacg tcagcagcat catggcggag aaggcgtgca cggcccaggc
     4681 aaaaccgtcg aggtacatca ggccgccgag cacgaggcac agcacgccgc cgctgctgtg
     4741 gatgagggct cccacatcca gcttgcccgc ctggtgttgc tgctcggcca tttggccctg
     4801 ccacccgtgt tctgcggcct gcgcaggcct tgaccgatca agttagcacg aaacgcggcc
     4861 ggtggcaggc caggtttgaa agtctggtac attgagcacc cgtccggggg gccaccacgt
     4921 gacgtgacac acaatcaatc gggggtaaca ccgtgacaaa atcatccgcc tacgtcccgc
     4981 cgaaagtctg gaagtgggac accgagagcg gtggacgctt cgccaacatc aaccggccaa
     5041 ctgctggcgc gacgcacgac aaggagctgc cggtcgggga gcatccgctg cagctctact
     5101 ccctggcgac accgaacggc gtgaaggtca ccgtgatgct ggaggaactc ctcgatctcg
     5161 gcaaaaccga cgcggagtac gatgcctggc tgatcaacat cggcgacggg gaacagttcg
     5221 gaagcgggtt cgtggcgatc aaccccaatt ccaagatccc cgccctgctc gatcgcagca
     5281 ccgagacgcc cacacgggta ttcgagtctg ccgccatcct gctctacctg gcagaaaagt
     5341 tcggcgcttt cctgcccgag gacctctcct cccggggcga gtgcttctcc tggctgttct
     5401 ggcagatggg cagcgcgccc tacctgggcg gaggtttcgg ccacttctac gcctacgcgc
     5461 cggagaaatt cgagtacccg atcaaccgct acaccatgga ggtcaagcgc cagctggacg
     5521 tgctcgatca gaacctggcg cagcgccgct atctgatcgg cgacgagtac accatcgccg
     5581 acatggccac ctatccctgg tacggcgccg tggtgctgca caacatctac gacgccgccg
     5641 agtttctcga cgtcacgtcc tacgagcacg tgattcgctg ggccaaggag atcgaggagc
     5701 gccccgccgt tcagcgcggc cgccgggtca acaaggtctg ggggtcggag gagacccaga
     5761 tgccagagcg tcacagcgcc agcgatttcg ggtcggtttg atgggcttgt cgaggtcaga
     5821 agaagc
//
LOCUS       DBNHLM010000248         3066 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_992701, whole genome shotgun sequence.
ACCESSION   DBNHLM010000248 DBNHLM010000000
VERSION     DBNHLM010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_992701"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..163
                     /locus_tag="AC16QF_11420"
                     /pseudo
     CDS             1..163
                     /locus_tag="AC16QF_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226942.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            276..464
                     /locus_tag="AC16QF_11425"
     CDS             276..464
                     /locus_tag="AC16QF_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449211.1"
                     /translation="MAYKQPRAPEDLVHFLTIELRIGKDGAINQTSGPISHLPETCWH
                     ELPLKTLQAKKPGACVSF"
     gene            complement(465..1553)
                     /locus_tag="AC16QF_11430"
     CDS             complement(465..1553)
                     /locus_tag="AC16QF_11430"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR6449212.1"
                     /translation="MQRPFFLALAALAALLPGTQMPQAEPTIDCVPKGNARPICGFSN
                     PEDIVALPGDQAILIGEYGASAEATGRLLVLELESEKQHTVFRGGQGDGAAEPGWGDP
                     TCTTPPGETFNSHGIDLVRRDDGRLQLLVIQHGSREAVELFEVMGAGTTWRVEWRGCV
                     PAPPNASLNSVAATSNGDFYTTQMTPLEPPVDLTKGIPRHVTGHAYAWSQSETSFRKI
                     GGTDGAMPNGIVTSPDGRFIYMNATAEHSVRKVEVATGRETGRATVDTPDNARWAPDG
                     RILIASLARDLTGEDFEACVSIERGACGIPFKIVALDPDSMTIVETLYATDGAPMGAG
                     TVGLRVGDELFIGSFHGDRILRVDLKGQ"
     gene            1763..2932
                     /locus_tag="AC16QF_11435"
     CDS             1763..2932
                     /locus_tag="AC16QF_11435"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6449213.1"
                     /translation="MNGSYLKTGLASALLWLLAAPVGAIDAPGVFEDRIVFGQSAAFE
                     GPAAALGTGMREGILAAFQEANRAGGINGRRLELISYDDGYEPEAAIANTKRLLDVDQ
                     VFALIGQVGTPTSEAVQPIATGQMVPFLGPFTGAAFLRQPSLGNVVNVRASYDQETEA
                     WIEHLTSDLGFSRIAIFYQDDSFGQAGLNGVRKALEKRRMALVAEGTYQRNTKAVEPA
                     VSTIRKARPEAVVMVGAYEPCAAFIKLAQENEMSAVFVNISFVGSKALSKELGPAGEG
                     VIVTEVMPLPDDTSNALVARYQRALQATKPDAEPGFVSLEGYVAGRLVVEALRQLGEP
                     VTREGMLAKIKTVGKFDLDGVTLTFGPEDNQGLDQVFLTVIQADGSFKAVERLER"
BASE COUNT          604 a          906 c          962 g          594 t
ORIGIN      
        1 caagctgcgg ggtcggttcc cgaagctcgg cgagctcatg gatcaggctg aggaggacgt
       61 gctggcgttc atgcactttc ccaaggagca ctggccgcag ctggcctcga caaacacgct
      121 ggaacggttg aacaaggagc tcaaacgccg gtctcgggtg atcaacagaa aacagacgca
      181 gctgctcgag ggagaagctg catgagatcc aggtcaacgg agacccagag ttacaccact
      241 tgacgggaca ctagcggatt ggggatctac cccacatggc ctacaaacag ccacgcgcgc
      301 ccgaagatct cgtccatttc ctgacgatag agctcaggat cggaaaagac ggtgcgatca
      361 accagacctc gggtccaatc agccatttgc cggaaacgtg ttggcatgaa ttgcccctta
      421 aaaccctgca agcaaaaaaa ccaggcgcct gcgtttcatt ctgattactg ccccttcaga
      481 tccacgcgaa ggatccgatc accgtggaac gagccgatga aaagctcgtc gccaacgcgc
      541 agcccaaccg ttccggctcc cataggcgca ccgtcggtcg catacagggt ttcgacgatg
      601 gtcatcgagt cggggtcgag cgcgacaatc ttgaacggaa tgccgcaggc tccccgctca
      661 atgctcacgc aggcctcgaa gtcttcccct gtgaggtccc tggcgaggga tgcgatgagt
      721 atgcggccat caggggccca gcgggcgttg tcgggtgtgt cgaccgtcgc gcggccggtt
      781 tcacggccgg tcgcgacttc gaccttgcgt acactgtgct cggcggtcgc gttcatgtag
      841 atgaagcggc catcgggtga ggtgacgatc ccgttcggca tcgcgccatc cgtcccgccg
      901 atctttcgaa aacttgtctc ggactgcgac caggcgtatg cgtggcctgt aacgtgacgc
      961 ggtatgccct tcgtgagatc gactggcggt tcgagcggcg tcatctgcgt cgtgtagaaa
     1021 tcgccattgg aggtggctgc gacgctgttg agcgacgcgt tggggggtgc cggcacacag
     1081 ccgcgccatt ccacccgcca ggtggttccc gcgcccatca cctcgaacag ctctacagct
     1141 tcgcgactgc cgtgctggat aaccagcagc tgcagacgcc cgtcgtcccg gcggacgaga
     1201 tcgatcccat ggctgttgaa cgtctcacca ggtggtgtcg tgcaggtcgg gtcaccccag
     1261 cccggctcgg ccgcgccgtc gccttgcccg ccgcgaaaca ccgtgtgttg cttctcgctc
     1321 tcgagttcca aaaccagaag cctgccagtg gcttccgccg aggccccgta ctccccgatg
     1381 aggatggcct ggtcccccgg tagcgcgacg atgtcctcag gattcgaaaa accgcagatc
     1441 ggtcgcgcat tgccttttgg tacgcagtcg atggtgggtt ccgcttgggg catctgggta
     1501 ccgggcagca gagccgctag cgcggcaagg gcaagaaaga acgggcgttg catgatggac
     1561 cgtcctgcgt tcatacaatt ggagaacgcc cgatgatgcc aaggccgcat tgcgtgagca
     1621 atcggtccag tctggtgaac tcccagccac ttgcgttaca caggcagctg ttttggagct
     1681 gaacgcccgg caggataaca tcgtgccgat tgatttctgc agcgctcgat ttggcttcta
     1741 attggtggaa ggggaagagc tcatgaacgg gtcgtatctc aagacaggcc tggcatcagc
     1801 gttgttgtgg ttgctggccg cgcccgtcgg cgcgatcgat gcgccgggag ttttcgaaga
     1861 ccggatcgtg ttcggccaat ccgccgcatt tgaggggccg gcggctgcgt tgggcaccgg
     1921 catgcgcgag ggtatcctcg cggcttttca ggaagcgaat cgcgccggcg ggatcaacgg
     1981 tcgcaggctc gagctcatct cctatgacga cggctatgag ccggaagccg ccatcgccaa
     2041 caccaaacgt ctgctcgatg tggaccaggt cttcgctctc atcggccagg tgggaacgcc
     2101 cacgtccgag gccgttcagc cgatagccac cggtcagatg gtgccgtttc tagggccctt
     2161 cacgggtgct gcctttctgc gacaaccctc ccttgggaac gtggtcaacg tgcgtgcatc
     2221 ctacgatcag gagaccgagg cctggatcga acatttgacc agtgacctgg gcttttctcg
     2281 gatcgccatc ttttatcagg acgattcctt cggccaggcg ggactcaatg gcgtgcgaaa
     2341 ggcgctggaa aaacgtcgga tggcgctcgt ggccgaaggc acctaccagc gcaacaccaa
     2401 agccgtggag cctgctgttt cgacgattcg gaaggcgcgg ccggaagcgg tggtgatggt
     2461 cggagcctac gaaccctgtg ccgctttcat caagctggcg caagagaacg aaatgagcgc
     2521 cgtcttcgtt aacatctcct ttgtcggcag caaggcgctc agcaaggagc tcgggcccgc
     2581 gggcgaagga gtgatcgtga ccgaggtgat gccgctgccg gacgatacga gcaatgctct
     2641 ggtggctcgc tatcagcgcg cccttcaggc cacgaaaccg gacgcggaac ctggctttgt
     2701 ctcactggag ggttacgtgg ctggccgtct ggtcgtggag gccctgcgac aacttggcga
     2761 gccggtgaca cgcgagggca tgcttgcgaa gataaagacg gttgggaagt tcgatctgga
     2821 cggcgtcacg ctgaccttcg gcccagagga caaccagggc ttggatcagg tcttcctcac
     2881 cgtcattcag gctgatggca gcttcaaggc ggtcgaacga ctggaaaggt agcgccagca
     2941 ggggaagctg cgaggtcagc gacaagatct ggcttgtcag cttcatggag tacgatccgg
     3001 agcccaaaca ttatgaaact accaattaag attctgctca tcgttcttgg cctgtacgtc
     3061 gccctg
//
LOCUS       DBNHLM010000249        54028 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1233321, whole genome shotgun sequence.
ACCESSION   DBNHLM010000249 DBNHLM010000000
VERSION     DBNHLM010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 54028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 54028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54028
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1233321"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="AC16QF_11440"
     CDS             complement(<1..723)
                     /locus_tag="AC16QF_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HIR6449214.1"
                     /translation="MLFSIALLLPVVVAVYYGESTTRTFALAFAITLISGLALTLVAR
                     SRRELRSGDGFLITVLFYLGLGLFGAIPIYLAESVDASFTDAAFESLSGLTTTGATVL
                     TGLDELPRSILFYRQLLQWLGGMGIIVLAVAILPMLGIGGMQLYRAESPGPVKDAKLT
                     PRITETAKALWYLYVGITASCALAYYAAGMDAFDALCHSFSTVAIGGFSTHDASIGYF
                     NSPVIDLVAIVFMFIAGINFSLH"
     gene            complement(765..2138)
                     /gene="trkA"
                     /locus_tag="AC16QF_11445"
     CDS             complement(765..2138)
                     /gene="trkA"
                     /locus_tag="AC16QF_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016489939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HIR6449215.1"
                     /translation="MKILILGAGQVGGTLAENLAKESIDITLVDNNIEALEELQTKLD
                     IKTVFGSASHPDVLRKAGADDADMLIAVTSSDEVNMVACQVCYSLFRTPQKIARVRSS
                     AYLKGEGFFSGEHMPIDVLINPEEVVTENIRQLLEHPGALQVLGFADDRVQLVAVKAY
                     YGGPLVGQELRFLRQHMPNIDTRVAAIFRRGRAITPEGNTVIEADDEVFFIAAAEHIN
                     AVMSELRRVERPFKRVMIAGGGNIGVKLAQSIEPYFSVKVLEQNARRCEELAEVLDRS
                     VVLNIDATDRELLLEENIEQCDAFCAVTNDDEVNIMSSLLAKRLGVRQVITLIGKPAY
                     VDLMQGGDIDIAISPQQATTSELLTHVRRADVVKVHSLRRGAAEAIEAIAHGDAKNSK
                     VVGRAIGDVDLPTGASIGAVVRGKEVFVGRDDVVVEPDDHVILFLADKRQVAAVERLF
                     QVGLTFF"
     gene            complement(2152..3420)
                     /gene="rsmB"
                     /locus_tag="AC16QF_11450"
     CDS             complement(2152..3420)
                     /gene="rsmB"
                     /locus_tag="AC16QF_11450"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF008149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIR6449216.1"
                     /translation="MPSPAEGAPATASRLLLAVLRDKQTSDQALERIPSTPQVRDTFY
                     GTLRHVFCLQRGVDKALRKPLANKHRDVYCLMLVGAYQLAYARVPDHAAINETVAGCR
                     ALRKPWATGLVNAVLRHIQRKGLPNDCSDASEHPAWMAERIRRDFPDRAGAIFCANMG
                     RAPMCVRINRRLVATDGYRQRLADSGIAFSEGFQDEFIILTAAMPTARLPGFDDGLAA
                     IQDAGAGFAADILAPEPGSRVLDACAAPGGKLFHLVERFPDAATLAIDTSTARLERLE
                     AEAARLHHNVNAQQGDATDLRWWDGQPFDRVLLDAPCTGSGTLRRHPDIRLLRAASDL
                     DDYARLQGRLLTNLWQTLRPGGNLLYCTCSLFAEENDEVIGAFLKAADDAEARPLSLA
                     TGVARAHGWQLLPTDSRTDGFYYALLTKRV"
     gene            complement(3404..4369)
                     /gene="fmt"
                     /locus_tag="AC16QF_11455"
     CDS             complement(3404..4369)
                     /gene="fmt"
                     /locus_tag="AC16QF_11455"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR6449217.1"
                     /translation="MTRPGGSAPRLGFAGTPEFAAAILQALVTAGHSPVVVYTQPDRR
                     SGRGRKALPSPVKALAAKQGIETRQPATLRETGAAADLAGLELDTLVVAAYGLILPQA
                     ILSAPRYGCMNVHASLLPRWRGAAPVERAIMAGDEETGVTIMQMDAGLDTGPIIEQER
                     CPIESATTGGELEATLARLGARCLLDCLGNLGSLDPTPQDEAGATYAHKLTPADASID
                     WNRPAEAIERQIRALNPRLPATCASGDLRIRILQARAIRDPGGEPGRIVSADRNGIRV
                     SCRESTLVIQSVQLDRGRGRPLSAADAVNGYAAYFEEGTKLAQPS"
     gene            complement(4366..4869)
                     /gene="def"
                     /locus_tag="AC16QF_11460"
     CDS             complement(4366..4869)
                     /gene="def"
                     /locus_tag="AC16QF_11460"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010430165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR6449218.1"
                     /translation="MATLDILEYPDPRLRTQAKPVTEVDAQVQQLVEDMFETMYAAPG
                     IGLAASQVNVHQRIIVIDVSENRDEPHVFINPELSLQGAAIESEEGCLSVPGFYEPVI
                     RSEQVIVSALDRDGQSFTLEAQGMLAVCIQHECDHLEGKLFVDYLSNLKRTRIRKKLD
                     KQRRVRA"
     gene            5166..6239
                     /locus_tag="AC16QF_11465"
     CDS             5166..6239
                     /locus_tag="AC16QF_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010796361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6449219.1"
                     /translation="MRKLRIIFQVLIGIGLGISASAWSATDGLAQYLKPGHPEVYTVV
                     KGDTLWDISGKFLDRPWLWPEIWRINPQIDNPHLIYPGDQIALEWVEGQPRLSLRRGD
                     AARTYKMTPADSVSLQPQIRSTPLESAIPTIRLEAIQGFLVQNRVVDPKVLSSAPYVV
                     QGENERLVLGAGDRLYVRGVLPDSESFNVVRKGPLYVDPETKEVLGQEATYIGLGRAV
                     AQEDDIATMSLQSTREEAQIGDRVLPTEERRVDSTFFPSAPDGDVNGQIISVFGGVTQ
                     VGQYDVVVLNRGEREGIEVGNVMAIYKRGALVKDRIANQTIRLPSERAGLLMVFRAFE
                     KLSYGLVLETERPLAVNDEVRNP"
     gene            6348..7436
                     /gene="dprA"
                     /locus_tag="AC16QF_11470"
     CDS             6348..7436
                     /gene="dprA"
                     /locus_tag="AC16QF_11470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR6449220.1"
                     /translation="MGDLAGDVESENAAAACLAMELGRPRARAAIAAHGNATSAVEAR
                     AASRDWNPRLVPQRLLADCARHGISLLGGEDACFPELLRHIPDPPLALFSLGALPGRD
                     GTAVAVVGARRCSRLGREVAYQLARQIATAGVRVVSGLALGIDKAAHEGALDAPGAGR
                     TVAVLGCGLARIYPRRNQALAERVVGEGGTLISEYPPEVPPAKHRFPERNRLISGLSH
                     GVVVVEAGDRSGSLITARLALEQGREVMAVPGPVSGGTSRGCHRLLREGAALVECAEH
                     VFEALNLTRPTPGSAETEQRAGLDLGEPLNSVLSAVEPVTTTMDQLVAVTGMAPEALS
                     VCLVDLELAGFVERVHEGYIRRPFSPGI"
     gene            7461..7961
                     /gene="purE"
                     /locus_tag="AC16QF_11475"
     CDS             7461..7961
                     /gene="purE"
                     /locus_tag="AC16QF_11475"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR6449221.1"
                     /translation="MSFVAILMGSNSDWPVMQSTTEVLGALDVEFEVRVTSAHRTPAA
                     TMEYVQDATQRGCAVFICAAGMAAHLAGAVAAHTTRPVIGVPIDSGPLSGFDALLSTV
                     QMPGGIPVATVAVGKAGAKNAGYLAAQILGLGDAALADRVASERAENARKVQSQNEQL
                     QEELGR"
     gene            7961..8518
                     /locus_tag="AC16QF_11480"
     CDS             7961..8518
                     /locus_tag="AC16QF_11480"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR6449222.1"
                     /translation="MTSVHVARAVRGLKAGGVVLHATEGVWGLACDPFDVEAVARVLV
                     LKGRPVAKGLILVGGNRDHFAEELDLLEPAVRSQVESTWPGAVTWVLPSTRFPEWISG
                     GRETVAVRVSGHPQVRALCERFGGALVSTSANPTGRLPATSQIRARAYFQGRVDYVLP
                     GEVLNAGAPSRIQTLGGEVLRGGGP"
     gene            8515..9378
                     /gene="aroE"
                     /locus_tag="AC16QF_11485"
     CDS             8515..9378
                     /gene="aroE"
                     /locus_tag="AC16QF_11485"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014869363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR6449223.1"
                     /translation="MSAGKPGEPDRYAVIGHPVSHSLSPAIHAMFAEQTGQHLAYAKL
                     PAPRDGFARTASDFFAGGGSGLNVTVPFKQEAAGWVDLLEGAAREAGAVNTISLESGQ
                     TKGFNTDGLGLVRDLTVNHGLSISGARVLVVGAGGATQGVVRPLIALGPQALTVANRT
                     VSKAAELVGSLGSAAGRARVAACAPEAATGPFDLVINATSAGLEGHGEVVPADAAAGA
                     VCYDMVYSTGAGAATAFCEWALGVGAVRAIDGLGMLVEQAAEAFLIWRGVRPDTAPVI
                     DALRRVAPPAY"
     gene            9399..9638
                     /locus_tag="AC16QF_11490"
     CDS             9399..9638
                     /locus_tag="AC16QF_11490"
                     /inference="COORDINATES: protein motif:HMM:NF021072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HIR6449224.1"
                     /translation="MTIKKRFIAGAVCPDCGAQDRLVVEEMPDGKRRRCVSCGFTDVS
                     ASEPEVALPTRFARRGVDSEQARPVRLLGEGDSDS"
     gene            10016..11131
                     /gene="coxB"
                     /locus_tag="AC16QF_11495"
     CDS             10016..11131
                     /gene="coxB"
                     /locus_tag="AC16QF_11495"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR6449225.1"
                     /translation="MGLGLAAAARADELNMTPGVTQISRSIYDLHMVIFWICVAIGVV
                     VFGVMFWSIFQHRKSRGAQAASFHESTRLEIAWTLIPILILIGMAFPATQTLVAMYDT
                     GGEDMAVEVRGYQWKWQYKYLDEDYNSTFSFFSNLATSRDEIENRVVKNDNYLLEVDE
                     PLRIPANRKVRFLITSEDVIHAWWVPDFGIKRDAIPGMLNELWTVVETPGVYRGQCTE
                     LCGMDHGFMPIVVEVMAEEDYDAWYADRVRMEEARHEALSKTFTPAELMEEGERVYAT
                     FCASCHQANGKGLPPVFPALAGSPIATGPLSDHLDIIVNGRAGTSMNAFGNQLDAAEI
                     AAVTHYERNAWGNDVGDVTQPIDVIEFMSGKEAEETP"
     gene            11128..12729
                     /gene="ctaD"
                     /locus_tag="AC16QF_11500"
     CDS             11128..12729
                     /gene="ctaD"
                     /locus_tag="AC16QF_11500"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR6449226.1"
                     /translation="MSEAVLTHHDDHHHGPASGITRWLFTTNHKDIGTLYLWFSFLML
                     LIGGAMALVIRTELFQPGLQFVEPAFFNQMTTNHGLIMVFGAIMPAFVGLANWLLPMM
                     VGAPDMALPRMNNLSFWILPFAFGMLISTLFMEGGGPNFGWTFYAPLSTTYAPDTVTY
                     FIFGVHLMGASSIMGSINIIATILNMRAPGMTLMKMPLFVWTWLITAYLLLAVMPVLA
                     GAVTMMLFDIHFGTSFFNAAGGGDPVLFQHIFWFFGHPEVYIMILPAFGVVSQIIPTF
                     ARKPLFGYDSMVYATASIAFLSFIVWAHHMFTVGMPLAGQLFFMYATMLIAVPTGVKV
                     FNWVSTMWRGSMTFETPMLFAIAFVVLFTIGGFSGLMLAIAPADYQYHDTYFVVAHFH
                     YVLVPGALFSIIAAVYYWLPKWCGRMYDETLGKVHFWLSFIGVNVTFFPQHFSGLAGM
                     PRRIPDYALQFADFNMVSSVGAFIFGFSQLVFLYVVIKAIRSGRPATDRVWEGAHGLE
                     WTLPSPAPYHSFTTPPTVTEAEAHS"
     gene            12769..13305
                     /locus_tag="AC16QF_11505"
     CDS             12769..13305
                     /locus_tag="AC16QF_11505"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HIR6449227.1"
                     /translation="MHKSSKGIVWKLGLMVVGMFGFGFALVPLYDLLCEVTGLGGRTG
                     GPYAYDPAVVEVDRSRLVKVNLLTNTNDGMPWRFWSEVGGMRVHPGEAKDVKFYVKNT
                     TDRRMVGQAVPSVVPITATDYFHKTECFCFESQVLEPGEEMELPLRFIVDAGLPKNVQ
                     TISLSYALFDATGFAAAE"
     gene            13334..14248
                     /locus_tag="AC16QF_11510"
     CDS             13334..14248
                     /locus_tag="AC16QF_11510"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR6449228.1"
                     /translation="MASSSDQATYYIPHSGWYPIWVAVGSALLLFGLGSWLNDLKAET
                     EPSRALFYTGAVILSVVLFAGFAKVVNENLEGLTSPQLHRSYIWGISWFIFSEVMFFF
                     AFFGALLYARVLSVQWLGGEGAKGFTGEYLWPEFEAVWPVISNPDPAVFENPRGDMAS
                     PGLLSWGGYLPFWNTVILLTSSVTVHFAHTAIKNADRTRLLRWLGLTVALGVVFLFLQ
                     VEEYVHAYQELGLTLSSGIYGSTFFLLTGFHGFHVTLGTFMLFVQLVRAFRGHFQPKD
                     CFGFEASSWYWHFVDVVWVCLFLFVYVF"
     gene            complement(14255..14482)
                     /locus_tag="AC16QF_11515"
     CDS             complement(14255..14482)
                     /locus_tag="AC16QF_11515"
                     /inference="COORDINATES: protein motif:HMM:NF022587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2909 domain-containing protein"
                     /protein_id="HIR6449229.1"
                     /translation="MIIFLLAGVVLSLFSSLAFLFRDSERPESKRALYALGIRITLAG
                     ALLLTVLYGFATGELSMDSNAPWHQSQTSGD"
     gene            14594..15298
                     /locus_tag="AC16QF_11520"
     CDS             14594..15298
                     /locus_tag="AC16QF_11520"
                     /inference="COORDINATES: protein motif:HMM:NF014193.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial respiratory chain
                     complex IV assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HIR6449230.1"
                     /translation="MSLAVTLLVPLMLGLGFWQLDRADQKRAHQDTLFSRLASAPVAG
                     RDTLAPEPFQRVRLEGRFEADRYFLVDNQVADGVVGYWVVQSFSTPQGVRWLVNRGWI
                     AGGDRRDQLPRVSAPTGTVVTVGVAWPDLGLVPLLDEDDWPLAWPVRVQRLDVARMAE
                     RLEDAVPVEIRLETAQPGALRAAPLTVGLQAERHEGYAAQWFALAGVLIVGYAVYGLR
                     GAQRARRLNGRTDESR"
     gene            15295..15861
                     /locus_tag="AC16QF_11525"
     CDS             15295..15861
                     /locus_tag="AC16QF_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449231.1"
                     /translation="MTQAEDKTGDQRGRAGRSQLVVILLVAGFSMAGAYLVFFLASSG
                     GVWGTTNNGEFVTPAMTMEALDLRDAAGTSATADGHWWLWTVAPGGCGEQCRFAVERL
                     RQVHALLNKDASRVRRALVTSAPGLEPPPGLDVSDLERLVGPADGLASGIYIVDPIGN
                     LVLRYPLSDAGEAVLEDLKRLLKVSQIG"
     gene            15905..16837
                     /locus_tag="AC16QF_11530"
     CDS             15905..16837
                     /locus_tag="AC16QF_11530"
                     /EC_number="1.17.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR6449232.1"
                     /translation="MLVVVLGAYTRLADAGLGCPDWPGCYGFLTVPESAEDIARAETL
                     FPDAPVERAKAWWEMVHRYFAAGLGLLVLVMAGRAYLRREQGGPVKLPLALLALVILQ
                     GAFGAWTVTMKLWPQVVTAHLLGGFTTLSLLWLLCLRLGVGSGWEVSAKLRTPALIAL
                     IVLVMQIALGGWVTSNYAALACPDFPTCHGEWLPEMDIGEGFNFAQSVGPNYLGGMLE
                     SEARVAIQLGHRLGALVATLAVGWLGLLLLRTGSPLGWPVLVLLAVQVVLGILNVLLA
                     LPLSNATLHNGFGAVLLLALVTVNYRAYLGGMRS"
     gene            16834..17745
                     /gene="cyoE"
                     /locus_tag="AC16QF_11535"
     CDS             16834..17745
                     /gene="cyoE"
                     /locus_tag="AC16QF_11535"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016782738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR6449233.1"
                     /translation="MSEVSLQAASWSDYLEMTKPRVVLLMLLCALVGMFLAVPGMVPV
                     DVLVFGLVGIALVAGSAAVVNHIADAQIDARMARTQARPVATGRVGLLQGLLFSAVLG
                     VSGMVVLYFLVNPLTAWLNLASWIGYGLIYTLYLKRATPQNIVIGGLFGAAPPLFGWA
                     AVSNSVEPGALLLVLIIFAWTPPHFWALALDRKEEYASVDVPMLPVTHGERYTRLHIL
                     YYTLILVAVSLLPFVIGMSGWLYLAGATALGGGFIYWAVVLLRNRNPRAPLETFRYSI
                     VYLGLLFVALLADHYVFADHLVGSSLQ"
     gene            17742..18389
                     /locus_tag="AC16QF_11540"
     CDS             17742..18389
                     /locus_tag="AC16QF_11540"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR6449234.1"
                     /translation="MRSVTATVLLCLSLAAAAVGAFVYSVLREPVLSDAELRDLGVFV
                     LPRPRAIGAFQLESTRGGSFTAADLQGGWSFLFFGFTNCPDVCPTTMAVLAQAERELL
                     AGSPEDADRFAGVLVSVDPERDDVRTLGEYTAAFSPRFFGVTGPFDELASFARQLNVG
                     FGKVPAPEGSFSEYLVDHGAQIVIINPQGHYHGFIRAPHTADTIVQTFASLAARF"
     gene            complement(18525..19490)
                     /locus_tag="AC16QF_11545"
     CDS             complement(18525..19490)
                     /locus_tag="AC16QF_11545"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR6449235.1"
                     /translation="MNAITEPGHPGIEETLTRYGVGELISAWPGSAASRCAYRVRTRR
                     AGREHDYLLLRVQAPRFEAELAALEACDRAGLPVAKAIPNLDGQPVDTLNGETVILTR
                     RLPGRHVYNPTVRQVAALGRFMARFHEAGGNLGPLPAWAWDQRWLAEASAALDGRLSY
                     PSARLMEATVQQVVSMLDREDVAGLPAGAIHGDICRDNVLFNEHGLSGVGGFSQVSSG
                     FLVYDLAVAANDWCIDANGMLDPERTLGFLRAYQKLRPLVRAELWFFSGFTLYAALNG
                     WIKRLAFAQGRQVSPGLHFNTPLELEKVVEQHAAHFFYLDERLLT"
     gene            complement(19521..19781)
                     /locus_tag="AC16QF_11550"
     CDS             complement(19521..19781)
                     /locus_tag="AC16QF_11550"
                     /inference="COORDINATES: protein motif:HMM:NF022639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HIR6449236.1"
                     /translation="MAAAATLPPTALASEAQDGLQGPDITIIAQEDKTILEFRQAGQL
                     RMVKIVPRLGKPYYLVPRDSTTGFGDLERADMLLPSWVIVEF"
     gene            19906..22632
                     /gene="polA"
                     /locus_tag="AC16QF_11555"
     CDS             19906..22632
                     /gene="polA"
                     /locus_tag="AC16QF_11555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR6449237.1"
                     /translation="MASDETAGAVSPPLLLVDGSSYLFRAYHALPELKTSTGLHTGAV
                     RGVIGMLRRLAKDYRESPIAVVFDAKGKTFRDDIYVQYKANRPPMPDDLSEQIEPIHQ
                     IIQAMGLPLIVVPGVEADDVIGTLAAEATRKRRDTVISTSDKDMAQLVTEHVTLVNTM
                     SETTLDPEGVVDKFGVPPELIIDYLALMGDSVDNIPGVPKVGPKTAAKWLNEYGDLDG
                     VVASAEQIKGKVGESLRDHLDQLPLSKALTTIKCDVALEVGIDDLAPRPVDEDRLKGL
                     FERLEFRSWLAEVGGPQEDAPAPAAASAEAEGEYECVSSAEALDEWIERLRAAPLFSF
                     DTETTSLNYMLAELVGVSFSCEPGKAAYVPLAHDYPGAPDQLGVAAALDRLKPLLEDP
                     AVIKVGQNLKYDMSVLQRYDVQLAGVGFDTMLESYVLNSTASRHNMDDLAKNYLSRET
                     IHYEDIAGKGAKQLTFNQVPVEQAVSYAGEDADITLQLHQTLWPRLEKEPRLTEVFRE
                     IEMPLLSVLSRMERKGTLVDGALLEAHGRELAERMRGITEEAWTLAGEEFNLDSPKQL
                     QAILFDKLELPVLKKTPKGQPSTAEPVLVDLARDFDLPRVILEYRSMAKLKSTYTDKL
                     PLQINQRTGRIHTSYHQAVAATGRLSSSDPNLQNIPVRTPEGRRIRQAFVAPPGKRIV
                     AADYSQIELRIMAHLSADDGLRAAFAANRDIHRATAGEVFGVGVDAVTDDQRRSAKAI
                     NFGLIYGMSAFGLARQLGISRTLAQEYIDRYFERYPGVLAYMDETRARARDAGYVETV
                     FGRRLYLPEINARNMQRRQAAERAAINAPMQGTAADIIKKAMLAVDAWLAGSELDAVM
                     IMQVHDELVFEVADADLDHLLAGVRDHMQGAADLDVELVVDCGTGANWDEAH"
     gene            complement(22629..23411)
                     /gene="yihA"
                     /locus_tag="AC16QF_11560"
     CDS             complement(22629..23411)
                     /gene="yihA"
                     /locus_tag="AC16QF_11560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR6449238.1"
                     /translation="MAEAHGNQRICMALIPLHYPPPSGARGLYNNSAELAECNFKDHQ
                     GNRVTETERTHKANKDRGSADRLTVQFISSAPSLRKCPRPDGPEIALAGRSNAGKSSV
                     LNRITGSRTTAKVGKTPGRTRLLNFFAVEGGGRLVDLPGYGYAKAARSAQHEWQATVN
                     DYLSNREALRAVVLVSDVRHPGQRFDMELLAWADASELPLLVLLNKADKLGRNAQAKA
                     LKAMQRAAEAMPLVDVILFSATSGLGNDAVVAWLREHLNAGA"
     gene            23377..24012
                     /locus_tag="AC16QF_11565"
     CDS             23377..24012
                     /locus_tag="AC16QF_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6449239.1"
                     /translation="MQIRWLPCASAIVVCLGLWAPGLAWAAGDPEAGAAQAVVCAACH
                     GQDGASGVDPSYPNLAGQNEKYLYNQLVMIQKDDRKILLMTGQLIGKSDQDLQDLAAY
                     YASLPAKLGQAEGDDDSIALAEQIYRGGIAKKGVAACAACHAPSGEGNASAGFPAVGG
                     QAGSYTIAQLTAYREGRRQTDEVYGGMMRGVAGGLTDGEIAALADYLQGLH"
     gene            24144..24836
                     /locus_tag="AC16QF_11570"
     CDS             24144..24836
                     /locus_tag="AC16QF_11570"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HIR6449240.1"
                     /translation="MRDVLAFLLVVALGATGPAFAQEWQEGVHYQRLPVSVATADAAR
                     VEVVEVFSYACIHCKTFDPALEAWRENQPEDVGFRRVPAIFSAEWETLAKAFYTAQIL
                     DLGGAVHDSIFRAVHEEGRDLREPGAIAALFQQVAGVNPQQFAQVFNSFGVRSRIEQA
                     KAHARAYRVSGVPALVVDGTYRVDGRMAGSNAAMLEVVNHLVAKQRAARGIAAPEPAS
                     VEEEDAATSGAS"
     gene            24978..25772
                     /locus_tag="AC16QF_11575"
     CDS             24978..25772
                     /locus_tag="AC16QF_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017849031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR6449241.1"
                     /translation="MHLESDVAPEEAAHSLRLLTFNIQVGIRTTKYRHYVTKGWKHVL
                     PHEARAINIRHIADLVRDYDLVALQEIDGGSLRSGFINQVEYIADRARFPYWYTQCNR
                     DLGPFAQHGNGVLSRIAPVHMEDHKLPGAIPGRGAILMRFKLGESTVSLVVLHLSLGG
                     RSRQVQLEYVRELIDAEEHVVVMGDMNSHLAQLLFNSPLSETDLVPADQVHPTYPAWQ
                     PAFALDHVLVSPGLSIDDYEVLDCRLSDHRPIAVTLRLGEPVTIQQ"
     gene            25910..28561
                     /gene="aceE"
                     /locus_tag="AC16QF_11580"
     CDS             25910..28561
                     /gene="aceE"
                     /locus_tag="AC16QF_11580"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014603513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HIR6449242.1"
                     /translation="MAERNPDDWLSEQQEWCDAIDDVLAFRGEDEVKELLRRLQAHVS
                     HRGMILTDAALNTPYKNTIDVRDQPAYPGNIELEMKIGDIICWNAVAMVLQAYDSGSG
                     VGGHIATYLSAATMMEVGFNHVFRGASESYGGDQVHFQAHTSPGVYARAFLEGRLSEQ
                     ALTNFRRELAPGGGLPSYPHPRRLPWFWQMPTASMGLSTPSAIYQARFAKYLESRGLK
                     ANNGGKIWTFIGDGESDEPEVLGTINIASRERLDNLVLVINCNLQRLDGPVRGNGKII
                     QELERAFRGADWHVIKVIWGGGWDAILARDEHGILQERMEQAVDGDYQMYTVSSGDVV
                     REHWVETSPELEELMKTLSDEEIRSIKRGGQDHQKIYAAFARAANVRGKPCVVLLKTV
                     KGDGLGPGTEGSNTVHQKKQLNEQERQELAKRLGIPLTREEISRAAFYRPAEDSAELV
                     YLRDRRHSLGGYLPAREVRCAALEPPSVELFAAMLKGSDRPVTTTAAVVRMLSRLLRD
                     PALGRYIVPIVPDEARTFGMDGLFPQAGIYSPAGQRYQPVDAGTIAPYREAEDGQILQ
                     EGICETGAMASFLAAGTAYANYGLPMIPFYIFYSIFGFQRVGDMIWAAGDMMCRGFLL
                     GGTSGRTTLNGEGVQHQDGHSHLVANTIPNLKSYDPAFAFEIALLVREGIRRMYQLQE
                     DVFYYLTLTNQPYPMPAMPEGVEDGVLKGMYCFSRAPGAVVNLFGSGALMTEVLRAES
                     LLAEHGIACNVWSVTSYNELARQALASERARLLGVGDGDERAYAEALLEGEEGIFVAA
                     SDYQKSLPMSISRWVPGPYTVLGTDGFGLSEDRRVLRGYFEVSAEWIAFAALSSLAQN
                     NRLERDVALGFAAKAGLALDKANPAEG"
     gene            28728..29396
                     /locus_tag="AC16QF_11585"
     CDS             28728..29396
                     /locus_tag="AC16QF_11585"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR6449243.1"
                     /translation="MDLRFSQFEILTAAGAFFLCLAFICVFNEASDIQDDVGIRAQGE
                     AREHGLYWAGVEALGQRVRLTGAAPSDAQRLHAAAAVAALPGVTSVQNEIEVLGPDGA
                     CQRQMDSALAELGVTFKTGRAEIGDSSYPLIAELARTMGACATPIEIAVHTDSLGDAA
                     VNLKLSQRRADAVRKALVQRGVRPDRLVAAGYGETQPVASNARESGRRENRRVEFRVV
                     GGTA"
     gene            29393..30025
                     /locus_tag="AC16QF_11590"
     CDS             29393..30025
                     /locus_tag="AC16QF_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449244.1"
                     /translation="MIYLVVNIVGYLLVALVAGVGVGWLLHGSLAGKASGERRGLGSV
                     GVDDLELAARADAQDEQLRKLAATIRQKEREVEALSRELERRDRRFNAADKLAKISRR
                     LQQEAEQLKEQVRVLGEQLEAAREEAQGSHGASAAMEEVLRSEILELEGRLRESSQEQ
                     DRLAKTLEQENRKVEELERQRDLQNSSLQVLHKQLELARKSREGAAEAGG"
     gene            complement(30029..30247)
                     /locus_tag="AC16QF_11595"
     CDS             complement(30029..30247)
                     /locus_tag="AC16QF_11595"
                     /inference="COORDINATES: protein motif:HMM:NF022596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HIR6449245.1"
                     /translation="MSEVGKWLVLAGALTVIVGLLLQTGWLGWFGRLPGDIRVERPGL
                     SFYFPITSMLIVSAILSVAAWILRRFLG"
     gene            complement(30244..31488)
                     /locus_tag="AC16QF_11600"
     CDS             complement(30244..31488)
                     /locus_tag="AC16QF_11600"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6449246.1"
                     /translation="MTNFLLQRYPAFGFPRYRKYWLASLASVGATQLITLAQGWLVFE
                     LSGSALQLGILGAAVAAPNIAMTLIGGVFADRFDKRLILVWTSGAIATLLALLAWLDY
                     SGTVRVWHVLAVAAAVSLITGLDWPARVSIFPQLIDRPAFMSAVALNSIIWQATRMAM
                     PALGGIIIAASDTWVLFALGSGGFLTMMIVISGLHVTTSDARHGSPLEQIAEGVRFIV
                     NEELFKWLMLLTFIGMFFAGSYVQIMPVFADVLESGEAGYGYLLSAGGVGAVAGTLLV
                     GGVQEHARLGFVMLGGATLSAALTAVFALVAVGGWFIAALLATFFASVFTSVFMISSM
                     AVLQLVVPDDLRGRVMGIHAIGYSLIPLGGLFMGALAEAAGPALAVLIGCCVFGFAIA
                     GVAMTSTTIRGLDGRLIGHKQA"
     gene            31548..32282
                     /locus_tag="AC16QF_11605"
     CDS             31548..32282
                     /locus_tag="AC16QF_11605"
                     /inference="COORDINATES: protein motif:HMM:NF013934.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR6449247.1"
                     /translation="MLEDVTRFGSKADARQWAWDALDERRLARFPFPPHGRIPNFAGA
                     AAAAMRIFAQAPWSEAGAIKVNPDSPQLPVRFLALQRGIRVYVPTPKLAAGFILLDPA
                     RIPPERFREAAALKTMLRWARPVALEDLPQLDAIVTGCAAATSSGKRAGKGAGYSDLE
                     FAILRELGHDPVPVATTVHDVQLVEDFPIAHNDLPLSLICTPTRTIRVQAPPAAPAGI
                     QWGELSAEQLAAMPVLESLRRLKHGG"
     gene            32272..33546
                     /locus_tag="AC16QF_11610"
     CDS             32272..33546
                     /locus_tag="AC16QF_11610"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR6449248.1"
                     /translation="MAGERTLPVRLALAVALLVSSASAEPAAAGESTVIVLSWDGMRH
                     DYPDRGDFPGLARVISEGARAERLVTVFPSSTFPGHVSLATGTYPDRHGIVGNHFVDR
                     QRGVYRMDSDADWLLAEPIWIAAERQGVMAATYFWVGSESDWRGRGTRYRESPFDGSR
                     PEALKVDRILEWLQLPEGQRPRLIMSYWAGADRIGHRFGPDSERVTTQIAAQDLQLQR
                     LLQGLDLLDRWASTTLLIVSDHGMTASYRYTDLRGMLAAAGIGAYVTGSTVVHVYLDD
                     PDDHARAGELLGKIEGARAYPGDALPGWMRLSHAARTGDWLVVATPPLTFSRPAGWEG
                     AMVATLSMVGWTFGSHGYPPDHPEMPGMFLALGRGVPAGALLRAVHQVDLAATIAALL
                     GIEPPLESEGTPVPGLEIPVAARQAAKEDDDG"
     gene            33539..35146
                     /locus_tag="AC16QF_11615"
     CDS             33539..35146
                     /locus_tag="AC16QF_11615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6449249.1"
                     /translation="MAKWQQPRSFWTWGYRSDEPSEEERHQAAARVSERLGRVVEPPP
                     IPRIGEIALRKPRLSVPAGLRGWVTTDHEERVVHTHGGHPLELLSAVRGEFDAPPDAV
                     AHPRNETELEATLAWCDEHGHAAIPFGGATSVVWGVRVPESADAAVTIDMDHFHQVLE
                     IDEVSRAARIQAGVLGPDLENTLRPLGLTLRHFPQSFPWSTVGGWVATRSGGHYATNH
                     THIDDFVESVRMLTPAGWWESRRLPGSGAGPSPDRMVMGSEGILGVISECWLRLQKRP
                     VYRATAGVVFDSWEAGAEAARQIVQAKLWPANLRLLDPAEAAQSAGLGGGRSLLIVGF
                     ESADLPQGDLMRDALAIARGAGGLVDDDAVRVDDGSGKATGREGSVGAWRSAFIGVNA
                     GLTSGLGLLADTFETAITWDRWPEFDASVRERVGRVLEEVLGPGARLSCRFTHVYPDG
                     PAPYYSFSGVAGLGAEHEQWRVIKDEATSAVVDAGGTVTHHHAVGRMHRAGWADQHPP
                     LYAQALRAMKRTLDPNGILNPGVLIDP"
     gene            35247..36146
                     /locus_tag="AC16QF_11620"
     CDS             35247..36146
                     /locus_tag="AC16QF_11620"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006636 - unsaturated fatty acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HIR6449250.1"
                     /translation="MLATLSRQLDSERAMVRRGRGLDWLRIWPFIGLHLACLAVFWVG
                     VSPVAVVVAVACYLIRMFAITAFYHRYFAHKAFRTSRACQFVFAVLGAASTQRGPLWW
                     AAHHRRHHMHTDTADDPHRPQDGFLWSHMGWFLSGEHFQTDMARVRDWSVFPELVWLN
                     RFDTVVPILLGTAMFALGWGLERLAPGLGTSGWQMLIWGYFISTVALIHATLLVNSLA
                     HRSGRRCFDTRDESRNNWIVAVLTLGEGWHNNHHRFAGAARQGFYWWQLDVSFYLLKL
                     MSWLGLIWDLKPVPGSVLREGRS"
     gene            36143..37384
                     /locus_tag="AC16QF_11625"
     CDS             36143..37384
                     /locus_tag="AC16QF_11625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6449251.1"
                     /translation="MRIAVVGAGVSGIAAAYRLQQEADVVLFEGQGRLGGHTDTHAVL
                     VGGATHAVDSGFIVFNPDNYPRFSSWLDELGVESQPSDMSFGVSNQATGVEYGSRGLG
                     ALFCQRRNLVSPRFLRMLADLRRFYRDAVDYPLDESLTLGELLDRHGYGAGFVEDHIG
                     PMCGALWSAAPDAALAIPARHVIAFMTHHRMLQLDGRPQWRVIKGGSSTYLQAFLARF
                     SGEVRLADPVQAVARTASGVDITSASGTARFDAVVLACHSDEALGLLADPSEAERDVL
                     GAIGYQPNRVVVHSDDRVMPEHRPAWSSWNALVGAERPERCQVSYWMNLLQSLPGREN
                     FFVTLNPQMPLKRVWSVREYAHPVFTRRARAAQRRKAEISGVRNTWFCGAYWGWGFHE
                     DGFVSGMEVAEGMLGSMADAA"
     gene            37374..38132
                     /locus_tag="AC16QF_11630"
     CDS             37374..38132
                     /locus_tag="AC16QF_11630"
                     /inference="COORDINATES: protein motif:HMM:NF018770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HIR6449252.1"
                     /translation="MPPELLRDSVCEGWVYHRRAAPEHRFRYRVWMLWLDVARLGQGP
                     LRLLRRRLGLSPLGIAPKDFLDGSDDLLGAVRARLASHGYPAPARVFLLTQPRSWISG
                     FNPVSFYFCVGQAGLDCILAEINNTPWDERHTYVLDARGQSGEFSFSFPKAFHVSPFM
                     PMDLEYRWRFAIEPEAVKIAMGLFREGEETFNAGLRLSVVPMTRRRLLLGMARYPLQN
                     VATLARIYWQALRLYSKRSRFYPHPDRAEEAPAS"
     gene            38129..39382
                     /locus_tag="AC16QF_11635"
     CDS             38129..39382
                     /locus_tag="AC16QF_11635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6449253.1"
                     /translation="MTDNTLYGDGRRTAPSGPFAALARKALGRLRDGTIRLTEAGRTE
                     VFGCGAPEVHVEVRDPRFFRRLVMGGSVGAGESYMDADWSCDDLVSLIRIFARNRDAL
                     DVVDGAGSVLVRWANRSRHLLRRNTRRGSRRNIEAHYDLSNEFFETFLDGRMMYSSAV
                     FESPDATLEEASEAKLERLCRKLELGPEDHLLEIGSGWGGLAVYAAAQFGCRVTTVTI
                     SPAQLEATQARVRAAGLQGRVRAVLSDYRDLDGCYDKVVSVEMVEAVGHEFLDRYFES
                     LSGLTRPGGLIALQAITIADRRYRAALAEADFIKKHIFPGSFIPSVCVLVDAAARRTD
                     AVLVNLEDIGLDYARTLRAWRERLEARRGEIQSLGFDERFLRMWRFYLAYCEGGFLER
                     AISDVQLLFAKPAYRGQPWRGALDG"
     gene            39416..40447
                     /locus_tag="AC16QF_11640"
     CDS             39416..40447
                     /locus_tag="AC16QF_11640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HIR6449254.1"
                     /translation="MSILIDLAEARRFPDALVRAGIRRLVAQRLKDEGSQGVAAQAER
                     HQRLLRELQESPVALDTELANEQHYEVPAEFFRAVLGKHLKYSSCYWERGAADLDEAE
                     AHMLALYGERAELADGQRVLDLGCGWGSFTLWAAERFPGSRFTAVSNSNGQRAYIESQ
                     AAARGLKNVEVITADVNDLRLPGIYDRIVSIEMFEHMRNYALLLNRIAGWLASDGKLF
                     VHIFCHRHLLYPYETEGDGNWMGRYFFTSGLMPAADTLLFFQDDLRCERRWSVSGVNY
                     EKTARAWLDNLDANESRVVQALEGTYGAAEVARWVQRWRMFFMACEELFAYRGGEEWL
                     VCHYRFAPR"
     gene            40484..41779
                     /locus_tag="AC16QF_11645"
     CDS             40484..41779
                     /locus_tag="AC16QF_11645"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6449255.1"
                     /translation="MKPKGIRSKLVRAFFIQVTIISCVTLVGIAVAYNIIYGVMLREA
                     LNDEAAHFWSRYESDPSHSLPDVYNLQGFMAVDGDFSGVPEQYRGLAPVGFSRQPYED
                     AQALVHVSERDGERLFLVFEYDQVSALAFFFGIVPLALVLLLIYGTSFVTYRLSQRAV
                     SPIVRLAAYLEDFDFNANLEISRDLEPLRAHADAEVVSMIQAVEHFTTRLAAFVERER
                     IFTRDAGHELRTPVAIFKGSLDLLEQSGDRPAHEHKALARMRRTVSDMESLLETLLLL
                     AREEQVEMPREDVSVNALVAGQLELLQPMAERAENSLALHERAELRVRAPEKVLEIVL
                     GNLIRNAINYTHGGRVDVTVTDRSVQVGDTGVGMSGEELQNAFEPFYRVDESRGAIRG
                     HGLGLAIVKRLAHQAGWSISAHSKPGEGTRVEVAFFPDD"
     gene            complement(41781..42731)
                     /gene="folE2"
                     /locus_tag="AC16QF_11650"
     CDS             complement(41781..42731)
                     /gene="folE2"
                     /locus_tag="AC16QF_11650"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HIR6449256.1"
                     /translation="MSTPKPVELASMPDIAGRPHAKPQGTLDWVGMSDIHQPILIRAG
                     NVNQRVQARVQVYVDLNDPHAKGIHMSRLYLLLDEHARTRPLSVAGLKMLLGSMHSSH
                     LDLSSRAFVQFDFEYELRQMALLSDNAGWSSYPITVKGTLADGKMTLELGVEILYSST
                     CPCSAALARQLIQQAFADRFDSGRPIDFADVNEWLGTEQGIVATPHSQRSVAKVITQL
                     ADDVTELPIAALIDTLETAIGTPVQAAVKRQDEQEFALRNGQNLMFCEDAARKLQAAL
                     NDDPSFNDFWLRVEHHESLHPHDAVSIVTKGIDGGYLPIP"
     gene            complement(42811..43509)
                     /locus_tag="AC16QF_11655"
     CDS             complement(42811..43509)
                     /locus_tag="AC16QF_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6449257.1"
                     /translation="MSRPEGSILLVEDNADLAETVGAFLEAKNYVVDYAADGLLAVQL
                     ATSEAFDAIVLDIMLPGIDGIEVCRRLRRDARLSTPIIMLTARDQLQDKLDGFDVGAD
                     DYLVKPFDMPELVARIEALIRRGRGVSAAYNVGELSMDLDTMSVFRGDTEIPLSRTLF
                     DILKILLREHPKVVPRETIERELWGDDLPDSDTLRSHLYNLRKAVDRPFNEPLIETLA
                     GRGYRVRVPEPDAG"
     gene            43641..45962
                     /locus_tag="AC16QF_11660"
     CDS             43641..45962
                     /locus_tag="AC16QF_11660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HIR6449258.1"
                     /translation="MDATGFILQATYRIHAGVPVIYLFGRLESGETFLVRDHRQRPHF
                     YVRQEDARRAEALDGIALEASGARTFSGDAVCRVTVPTPSDAPPARERLHGLGVDTFE
                     ADVRFAMRYLIDRGIRGGCRIRGAASRGEGVGWVFDDPEVGAAEAGIEPVVLSFDIET
                     DAKADRLLGIAVYGAGVDEVLIVDPERRPMPERARGLPSERAVLEAFCQLVRDVDPDV
                     LTGWNVVDFDLTVLGRVAARVRHPFQLGRQAGALRIRPAQGYFGSGNATIPGRLVLDG
                     MDLLRGAFVRMEEYSLDAVAREVLGEGKTLDGDVRDRAGEIMQRYRQDLPGFARYART
                     DARLALDIVTRLDLMNLAFARSRLTGMTPDRVAASIASFDFLYLSALGKRGLVAPTVR
                     SDDSRVHAAQSGGQVFEPVTGVHRNVMVFDFKSLYPSIIRTFNVDPLGFVPSGDAEPD
                     VIRLVNGAGFRRDPAILPRMLDDLFPRREAAKTAGDSVASQAIKILMNSFYGVLGTPA
                     CRFHNVEIANAITGQGRHLLQWSKRWFETRGFDVLYGDTDSLFVRSRQGDEDAALAEA
                     RDLAARLNADIAAEVLERWGVVSRLELEFEKLYAKLFLPSVRHGAAGARKRYAGLPVG
                     AEEVEFVGMEVVRRDWTELAKRVQRELYARLFSGAAVDDYLAGVVAQLRRGELDELLV
                     YRKGLRKRLDSYTASNPPHVAAARKSGEPPGRTVAYLVTSAGPEPLDALQHEPDREHY
                     VDKQIKPVAEPVLGALGLEFARVIGDDRQMGLF"
     gene            complement(45993..47033)
                     /locus_tag="AC16QF_11665"
     CDS             complement(45993..47033)
                     /locus_tag="AC16QF_11665"
                     /inference="COORDINATES: protein motif:HMM:NF015715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HIR6449259.1"
                     /translation="MADVPTPASRLARYRTDYLLMTCAAIALVLLILALTGAAPMGTP
                     LGHAAHTVHEMLAAMWWGIALAILIIGVLERIPQEFIIALLGPPGSRNGVLRAAVAGV
                     LLDLCSHGILAVGAKLYQRGASAGQVMAFLIASPWNSVSLTFILVGLIGLGWTIAFIG
                     LSLVVAITTGLAFDRLARTGRIPANDAQVDLPVNFRFGPASRELVSTIEWEPRLLADI
                     VINGLRGTRIVLRWLMFGLVVAAALRAFLSPEDFAQWFGPSLLGLGLTLVGATIFEVC
                     SEGSVPIAADLMNRAGAPGNSFTFLLAGVASDYTEIMVLREATGSTKIALMLPAICVP
                     QVLLIGWVLNQL"
     gene            complement(47120..47638)
                     /locus_tag="AC16QF_11670"
     CDS             complement(47120..47638)
                     /locus_tag="AC16QF_11670"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6449260.1"
                     /translation="MDELFETYDEAGRAMGLMARHRVHATGAWHRSAHVFLFDTTGAL
                     LIQRRAPGKDLYPDLWDYSVGEHLVPGETYLAAARRGLEEELGVRGVSLEPIGDVRRW
                     RSHQAELNLRDNELQQAFTGVHDGPFEPDAAEVAEVRAVGLQELAAEIERWPARFTPW
                     FVRDLAELGFVK"
     gene            47745..48725
                     /locus_tag="AC16QF_11675"
     CDS             47745..48725
                     /locus_tag="AC16QF_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6449261.1"
                     /translation="MLKLQGIRLAEGAAPLDHEFRSGQITVVLGRNRSGKTRLCRLLA
                     GLEPTGEGRILLDGVDVSNIPAGQRPVSFVYQSFVNYPTLTVAENIASPMRARGLPSR
                     ERRRRVEDLAARLHISELLDRRPAQLSGGQQQRLAIARALAKQARVLVMDEPLVNLDF
                     KLREALELELRGLLRDAGIAVVYTSSDPRDAFNLGDEVLLLADHAPVQAGRPLDVYER
                     PVSVAAADLMSDPGVNLLPGPDRPAAIRPEHLHVSRHARDDETFAAELLTLETNGSET
                     YLHCRVADAHWVVKLEGLIDLKLRPGDTLPLFAEQRSLLRFSEAMTDVQD"
     gene            48712..49818
                     /locus_tag="AC16QF_11680"
     CDS             48712..49818
                     /locus_tag="AC16QF_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6449262.1"
                     /translation="MSRIELDGVSHRYPEAPSETFAELELVIGSGEAHALLGSSGAGK
                     TTLLNLLSGIEQPTTGRITFDGRDVSRTPPRARNVAQVFQFPVLYESLSVAENLEFPL
                     KTRGVRAAERRRRAKAMAERLGIAELMGRRPGKLSLFERQLVAIGRALVRPELSLVLL
                     DEPLTAVEPSIKWQLRRELKAVQQEIGATMIYVTHDQTEALTFADRISLLHRGRILQT
                     GSARTLFSAPAHEQVGHFIGSPGMNLVPGEIAEGALRLGGRVVAPAAGAEPGPCTIGF
                     RPEWASVAPSGPDGSGLLGTITAARVLGVRRGVPVGILSVDLGVGVVQVRQALSASPG
                     ERVGVNVDAGQVVAFREGWRLPGIGAETGRGAGS"
     gene            49815..50681
                     /locus_tag="AC16QF_11685"
     CDS             49815..50681
                     /locus_tag="AC16QF_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6449263.1"
                     /translation="MSGRRPDNRAWLGVLPVVLVVAFSAVIPLMTVVNYSLQDIFDQH
                     TRLFVGFDWYREILRDPRFLEALLRQLAFSGAVLLIEVPLGVMVARSLPRSGWLAPAL
                     VVLIALPLLVPWNVVGTIWQIFARPDIGLLGAALTGLGFEFNYTSSPVDAWITLLLMD
                     VWHWTSLVALLAYAGLASIQEAHYQAAEIDGAGRWQVFRLVELPRLRPVLTIAVLLRL
                     MDSFMIYTEPFVLTGGGPGNATTFLSQYLTSMAVGQFDLGPAAAFSVLYFLVTLLMCW
                     LFYTSVIRKEDE"
     gene            50678..51478
                     /locus_tag="AC16QF_11690"
     CDS             50678..51478
                     /locus_tag="AC16QF_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6449264.1"
                     /translation="MTRRSHWLIFIYCAYLLLPVYWLVNMALRSNRDIVGGLEIVPGR
                     PTLANFAEIVTNPVWYEGFANSLTYVGVNTVISLLVALPAAFAFSRFRFLGDRHLFFW
                     LLTNRMAPPAVFLLPFFQLYSAVGLFDTPLAVALAHCLFTVPLAVWILEGFMSAVPRS
                     LDEMARIDGHSLPYFFFRIFLPVIRGGVGVTVFFCFMFSWVELLLARTLTASAAKPIV
                     VVMTRTVSASGMDWGVLAAAGVLTIVPGVAVVWFVRRHLVKGFALGRV"
     gene            51498..51776
                     /locus_tag="AC16QF_11695"
     CDS             51498..51776
                     /locus_tag="AC16QF_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007946969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2160 domain-containing protein"
                     /protein_id="HIR6449265.1"
                     /translation="MSFSWMAWTLPTAIFFGAILCLLVVMTVLEIRWPTRTRRGLLPL
                     ATTRGDRLFIGLLVAAFVHILWLAALDAPVLLATGISLGLLALLIRWG"
     gene            51842..53608
                     /locus_tag="AC16QF_11700"
     CDS             51842..53608
                     /locus_tag="AC16QF_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012271558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6449266.1"
                     /translation="MRGIVLFLGAATLLLAGCDGSAPDAPGVDRHAAAKRWITQEFQP
                     STLTPDEQAAEMAWFIEAAAPFRGMEISVVSETLTTHEYEAGVLARAFAEITGIRVSH
                     DLIQEGDVIEKLQTQMQSGRNVYDAYVNDSDLIGTHARYGYVVALSDFMAGEGADVTL
                     PTLDVDDFIGRSFTTGPDGKLYQLPDQQFANLYWFRYDWFQREDLRARFRELYGYDLG
                     VPVNWSAYEDIAEFFSEYVRTIDGQRIYGHMDYGKKDPSLGWRFTDAWLSMAGAGDPG
                     IPNGLPVDEWGIRVDGCRPVGASVSRGGAANGPAAVYALEKYIDWLKRFAPPQAPGMT
                     FSEAGPVPAQGHIAQQIFWYTTFTADMIDGDLAVVDEDGLPRWRMAPSPHGPYWEEGM
                     KLGYQDAGAWTLLASTPLERRKAAWLYAQFVVSKTVSLKKTLVGLTPIRDSDLRSAAM
                     TEAAPRLGGLVEFYRSPARTLWTPTGTNVPDYPKLAQLWWPNVANAVSGAVSAQEAMD
                     TLARDQDRVLERLQRAGIQGDCGPELNPEVDPEEWLARPGAPKARLADEKPPGKTVPY
                     DELVADWGALPRPGEPIGRRSH"
     gene            53612..>54028
                     /locus_tag="AC16QF_11705"
     CDS             53612..>54028
                     /locus_tag="AC16QF_11705"
                     /inference="COORDINATES: protein motif:HMM:NF018649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIR6449267.1"
                     /translation="MAQPTQTARGGPGRGSAFGWIWIAYLAAVVVGVAGTMIVADPAT
                     DHPVYLAGIADVLATVVIFAFSVAFRNSSFYDPYWSVVPPVLGLYWWWVAGSVEDPRV
                     WLAMLVVSCWAVRLTYNWAFGWTGLGHEDWRYRDLQN"
BASE COUNT         8734 a        17171 c        18287 g         9836 t
ORIGIN      
        1 atgcagcgag aaattgatgc cggcgatgaa catgaacacg atcgccacga ggtcgatgac
       61 cgggctgttg aaatagccga tgctggcgtc gtgggtcgag aagccgccga tggcgacggt
      121 ggaaaagctg tggcaaaggg cgtcgaaggc gtccatcccg gcggcgtagt aagcgagcgc
      181 gcaggatgcc gtgatgccga cgtagagata ccacagggcc ttcgccgtct cggtgatgcg
      241 cggcgttagc ttggcatcct tgaccggccc cggggactcg gcgcgataca gctgcatgcc
      301 gccgatgccc agcatgggaa ggatcgccac cgcgagaacg atgatcccca taccgccgag
      361 ccactgaagc agctgccggt agaaaaggat ggaacggggg agctcgtcga gcccggtcag
      421 gaccgtcgcc ccggtggtcg tcagccccga cagcgactcg aaagcggcgt cggtgaaaga
      481 cgcatcgacg ctctcggcca ggtagatggg gattgccccg aacaggccga gacccaggta
      541 gaagagcacg gtgatgagaa acccgtcgcc gcttcgcaac tcgcgccggg accgtgcgac
      601 cagggtcagc gccaggcccg aaatcagggt gatggcgaaa gccagggcaa acgtgcgggt
      661 ggtgctctcc ccgtagtaga cggccaccac cacgggcagc aggagcgcaa tgctgaacag
      721 catcagcagc gttccggtca ctcggagaat gacggagaaa tgcatcagaa aaaggtcagc
      781 ccgacctgga acagccgttc caccgccgcc acctgacgct tgtccgccag aaacaggatc
      841 acgtggtcgt cgggctccac caccacgtcg tcacggccga cgaaaacttc cttgccccgg
      901 accacggcgc cgatggacgc gccggtcggc aggtcgacat ccccgatcgc ccggccaacc
      961 accttggagt ttttcgcgtc gccgtgggcg atggcctcga tggcttctgc agcaccgcgg
     1021 cgcaggctgt gcaccttgac cacgtcggcc cgtcgcacgt gggtcaggag ctcgctggtg
     1081 gtggcctgct gaggcgagat ggcgatgtcg atgtcgccgc cctgcatcag gtcgacgtag
     1141 gcgggcttgc cgatgagcgt gatcacctgt cggaccccga gccgtttggc cagcaacgag
     1201 gacatgatgt tgacctcgtc gtcgttggtg acggcgcaga aggcgtcgca ctgctcgatg
     1261 ttctcctcca gcagaagctc ccggtcggtg gcgtcgatgt tgaggaccac ggacctgtcg
     1321 agaacctcgg ccagctcctc gcagcggcgg gcgttctgct ccaacacctt caccgagaag
     1381 taaggctcga tgctctgggc aagcttgacg ccgatgttgc ccccgccggc gatcatcacg
     1441 cgcttgaagg gccgctccac ccgcctgagc tcgctcatca cggcgttgat gtgctcggcg
     1501 gcagcgatga agaagacctc gtcgtctgcc tcgatcaccg tgttgccctc gggcgtgatg
     1561 gcgcgaccgc gacggaagat ggccgccacg cgggtgtcta tgttcggcat gtgctggcgg
     1621 agaaagcgaa gctcctgacc gaccaggggg ccgccgtagt acgccttcac ggccaccagc
     1681 tggactctgt cgtcggcgaa cccgagaacc tgaagcgctc cggggtgctc gagcagctgt
     1741 cggatgtttt ctgtgacgac ctcctctggg ttgatcagca cgtcgatggg catgtgctcc
     1801 ccgctgaaga acccttcccc cttcagatat gctgacgagc gaacccgcgc gattttctgc
     1861 ggcgttcgaa acagggagta gcagacctgg caggcgacca tgttgacctc gtcgctggag
     1921 gtcaccgcaa tcagcatgtc ggcgtcgtcg gcgccggcct tgcgcagcac gtccgggtgg
     1981 ctcgcggacc cgaagacggt cttgatgtcc agcttggtct gcagctcctc gagggcttcg
     2041 atgttgttgt cgaccagcgt gatgtcgatg gattccttgg cgaggttctc ggccagcgtt
     2101 ccgcccacct gccccgctcc gagaatgaga atcttcatgt tggctgcctt gttacacccg
     2161 cttggtcaga agagcgtagt agaacccgtc ggtgcggctg tcggtgggca gaagctgcca
     2221 cccgtgagcc ctggccacgc cggtagccag cgacagaggg cgggcctcgg catcgtcagc
     2281 cgctttgagg aacgccccga tcacctcatc gttctcttcc gcaaacagcg agcacgtgca
     2341 gtaaagaagg ttaccgccgg ggcgcagggt ctgccacagg ttagtcagca gccgtccctg
     2401 cagtcgggcg tagtcgtcga ggtccgaggc tgcccgcagg agccttatgt cggggtgccg
     2461 gcgcagggtc ccgctgcccg tgcagggggc gtccagcagg acccgatcga agggctggcc
     2521 atcccaccac ctcaggtccg tggcatcccc ctgctgggcg ttcacgttgt gatggaggcg
     2581 agcggcctcg gcttcgagcc gttccaggcg tgccgtggag gtgtcgatgg ccagggtggc
     2641 cgcgtccggg aaccgctcga ccaggtggaa gagctttccg cccggggccg cgcaggcgtc
     2701 gagcaccctg gaccccggct ccggagccag gatgtctgcg gcgaatccgg cgcccgcgtc
     2761 ctggatggcc gccagcccat cgtcgaagcc tggcagccgg gccgtcggca tggccgccgt
     2821 cagaatgatg aactcgtcct gaaagccctc cgagaaggcg atgccggaat ccgcgagccg
     2881 ctgtcggtag ccgtccgtcg cgacgagtct cctgttgatg cgaacgcaca tgggcgcacg
     2941 gcccatgttg gcgcagaaga tggcgcccgc ccggtccgga aagtctcgac gaattcgttc
     3001 agccatccag gccggatgct ccgaagcatc cgaacaatcg ttcggcagac cctttcgctg
     3061 gatgtggcgg agaacggcgt tgaccagacc cgtagcccag ggcttgcgaa gcgcccggca
     3121 cccggcgacc gtctcgttga tggcggcgtg gtccggtacg cgggcgtatg ccagttggta
     3181 agcgcccact aacattaaac agtagacgtc ccgatgcttg ttcgccagag gcttgcgcag
     3241 cgccttgtcc acaccgcgtt gcaggcagaa aacgtgtcgc agggtgccgt agaacgtgtc
     3301 gcggacctgc ggtgtggacg ggatgcgctc cagagcctga tcggacgtct gcttgtccct
     3361 gagcacggcg agcagcaggc gcgacgccgt ggccggagcg ccttcagctg ggctgggcaa
     3421 gctttgtgcc ttcttcaaaa taggcggcgt agccgttgac agcgtcggct gcggacaggg
     3481 gacgcccgcg cccgcgatcg agttgcacgg actggatgac cagggtgctc tccctgcagg
     3541 agacgcggat accgttcctg tcagcggaga cgatccttcc gggttccccg ccagggtccc
     3601 ggatcgcccg cgcctggaga atgcggatgc gcaggtcgcc ggaggcgcag gtggcgggca
     3661 atcttggatt cagagctcgg atctggcgct cgatcgcttc ggcggggcgg ttccagtcga
     3721 tgctggcatc ggccggcgtc agcttgtggg cgtacgtcgc cccggcctca tcctggggcg
     3781 tggggtcgag gctgcccaga ttccccaggc agtccagcag gcagcgggcg ccaagccgcg
     3841 ccagcgttgc ttcgagctcg ccgccggtgg ttgcgctttc gatggggcac ctctcctgct
     3901 cgatgatcgg gccggtgtcc agaccggcgt ccatctgcat gatggtaacc cccgtctcct
     3961 cgtcccccgc catgatggct cgctcgaccg gcgcggcccc gcgccagcgc ggcagcagcg
     4021 aggcgtgaac gttcatgcac ccgtacctgg gcgcgctcag gatggcctgg gggaggatca
     4081 gtccatatgc agctaccacc agcgtgtcga gctcaagccc cgccagatcc gccgcggccc
     4141 cggtctccct cagcgtggcc ggctggcggg tctcgatgcc ctgcttcgcg gccagggcct
     4201 tgacggggct cgggagcgcc ttgcggccac gaccggagcg ccgatcgggc tgggtgtaca
     4261 cgaccaccgg cgagtggccg gccgtgacga gcgcttggag gatggcggcc gcgaactcgg
     4321 gtgtgcctgc aaacccaagg cgcggcgcgc tgccgcccgg cctggtcatg cgcggacgcg
     4381 gcgttgtttg tccagctttt tgcgaatccg cgtgcgcttc aggttggaca ggtaatccac
     4441 gaagagcttg ccctccaggt ggtcgcattc atgctggatg cagacggcca gcatgccctg
     4501 ggcctcgagc gtaaaggact gtccatccct gtcgagcgcg ctcactatta cctgctcgga
     4561 gcggatgacc ggctcgtaga accccggaac cgacaggcaa ccctcctccg actcgatggc
     4621 ggccccctgc agcgaaagct cgggattgat gaaaacgtgc ggctcgtctc ggttctcgga
     4681 aacgtcgatc acgatgatcc gttggtggac gttgacctgg ctggccgcca ggccaattcc
     4741 gggagcggcg tacatggtct cgaacatgtc ttcgaccagt tgctgtactt gcgcgtccac
     4801 ctcagtcacg ggtttggctt gagtgcgcag cctggggtcg ggatattcga gtatgtcgag
     4861 ggttgccatg gattgttgta gcgcctttgc ggcctatgtg atgtgtctct acttctgctg
     4921 caattcaggc tgataacatt gtgacgagta tatgtttttc gctcggaaac ccggctcggt
     4981 cgcgggctca agccttaccc ggcgacggtt gagcggatgt taatgagcct tcatttggac
     5041 ttaaaggcct acaactcgaa atggacagca tccggcggca agagttcgaa gcgagccagc
     5101 ctcggcgatc ctcttcaggg atgcttccgg cacgactagc aggtacagct aaaaggaaag
     5161 gaatcatgag gaaactgcgc atcattttcc aggttctgat cggaatcggt cttgggatct
     5221 cggcaagcgc atggagtgcc acggacggcc tggctcagta cctcaagccg gggcatccgg
     5281 aggtttacac cgtggtcaag ggcgacacgc tgtgggatat cagcggcaag ttcctggatc
     5341 ggccctggct ttggccggag atctggcgga taaaccccca gatcgataat ccccatctca
     5401 tctacccggg cgatcagatc gcgctcgagt gggtggaggg acagccccgg ttgtctctga
     5461 gaaggggtga cgcggcgcga acctacaaga tgacgccggc ggattcggtg agcctgcaac
     5521 cgcagattcg ctccaccccg ctggagtcgg ccatccccac cattcggctg gaggccattc
     5581 aggggttcct ggtgcagaat cgggtggtgg atcccaaggt tctgtccagc gcgccatacg
     5641 tcgtgcaggg cgaaaacgag cgcctggttc tgggcgccgg cgaccggctc tacgtgcggg
     5701 gcgtgctgcc ggacagcgaa agcttcaacg tcgtccgcaa agggcccctt tatgtggacc
     5761 cggagacgaa agaggtgctt ggccaggagg ctacctacat cgggctgggc agggccgtgg
     5821 cgcaggaaga cgatattgcc accatgtcgc tgcagagcac ccgcgaggaa gcccagatcg
     5881 gtgaccgagt gctgccaacc gaagagcggc gggttgactc cacgttcttc ccgagcgcgc
     5941 cggacgggga cgtcaacggc cagatcattt ccgtgttcgg tggcgtgacc caggtcggcc
     6001 agtacgacgt ggtggtgctc aatcgcggcg agcgggaggg catcgaggtc ggcaacgtca
     6061 tggcgatcta caagcgcggc gctcttgtca aggaccgtat cgccaatcag accatccggc
     6121 tccccagcga acgggccggt ctgctgatgg tcttccgggc gttcgagaag ctctcttacg
     6181 gcctggtgct cgagaccgag cgtccgctcg ccgtcaacga cgaggtacgt aatccctgat
     6241 ttgcgctacg gtgcggcgaa atcgcaccgc tgaccggccg ccgggctgac cggcggcaat
     6301 cagggtcttc aatagaatcg cccctcaagt cgaggggcga tttttttatg ggggacctgg
     6361 ctggggacgt ggagtcggag aacgcggcgg cggcctgcct tgccatggag cttggtcggc
     6421 cgcgggcacg ggcggcaatc gccgctcatg gcaatgcgac ctcggcggtc gaagctcggg
     6481 cagcgtcccg ggattggaac ccccgcctgg taccccaacg gctgctcgcg gattgtgccc
     6541 ggcacgggat ctcgctgctc ggcggggagg atgcgtgctt tcccgagctg ttgcggcaca
     6601 tccccgaccc gccgcttgca ctgttctctc tcggcgcgct gccgggacgc gacgggactg
     6661 ccgttgccgt ggtgggcgcc aggcgttgca gccggctcgg ccgggaggtc gcctaccagc
     6721 tcgcccggca aatcgcgacg gcgggcgtca gggtggtcag tgggctggcg ctcggtatcg
     6781 ataaagccgc ccacgaaggc gcgctcgatg cccctggggc cggtcggacc gttgcggtct
     6841 tgggctgcgg gcttgcccgg atctatccgc ggcgcaatca ggccctcgcc gagcgcgtgg
     6901 tgggcgaggg cggcacattg atcagcgagt acccgccgga agtgcccccc gcgaagcatc
     6961 ggtttcccga gcgcaacagg ctgatcagcg ggctctccca cggcgtggtc gtggtcgagg
     7021 ccggcgatcg cagcggctct ctgatcaccg cgcgactggc actcgagcag gggcgggagg
     7081 taatggccgt tcccggaccg gtgagcggcg ggacgagtcg aggctgccat cgcctgctgc
     7141 gggagggggc cgcgctggtg gagtgcgccg agcatgtctt cgaagcgctg aacctgaccc
     7201 gccccacccc cggatccgcc gagacggaac aaagggcggg tttggacctg ggcgagcctc
     7261 tgaacagcgt gctgtcggcg gtggagcctg tgaccaccac catggaccag ctggtcgccg
     7321 tcaccggcat ggctcccgag gccctgtcgg tgtgtctggt cgatctggag ctcgcgggat
     7381 ttgtcgagcg ggtccacgag ggttatattc ggcgaccttt ttcgccgggg atataaattt
     7441 ccggctcagg aggaaaaaac atgagctttg tggccatttt gatgggttcc aactccgact
     7501 ggccggtgat gcagagcacc accgaggtgc tcggcgccct ggacgtggag ttcgaggtgc
     7561 gggtcacctc tgcccaccgg accccggcgg cgaccatgga gtacgtgcag gacgcgaccc
     7621 agcgaggatg cgcggtcttc atctgcgccg ccggcatggc ggcccatctt gcgggcgccg
     7681 ttgcggcgca caccacccga ccggtgatcg gggtgcccat cgacagcggc ccgctttccg
     7741 gattcgacgc gttgctctcc acggtgcaga tgccgggcgg gatcccggtg gcgaccgtgg
     7801 ccgtgggcaa agcaggggcg aagaacgccg gttacctggc cgcgcagatc ctgggactgg
     7861 gtgatgcggc cctcgccgac cgggtcgcct ccgagcgggc ggagaacgca cgcaaagtcc
     7921 agtcccagaa cgagcagctg caggaagaac tcggccgctg atgacttccg tgcacgtggc
     7981 gcgcgccgtc cgagggctta aggctggcgg cgtcgtgctg cacgcgacgg agggtgtctg
     8041 gggccttgcc tgcgatcctt tcgacgtgga agcggtggct cgcgtactgg tgctcaaagg
     8101 tcgcccggtg gccaagggcc tgatccttgt tggcgggaac agggatcact tcgctgagga
     8161 gctcgacctg ctcgagccgg ctgtccggtc tcaggtcgaa agcacctggc ccggtgcggt
     8221 gacctgggtc cttcccagca cccgctttcc ggagtggatc agcggcggtc gggaaacggt
     8281 agccgttcgg gtctcgggtc atccccaggt gagagcgctg tgcgaacgct ttggcggagc
     8341 cctggtgtcc acctcggcga accccaccgg cagattgccg gccaccagcc agatcagggc
     8401 acgagcgtac ttccaggggc gcgtggatta cgtcctcccc ggcgaggttc tcaacgcggg
     8461 cgcccccagc cggatccaga ccctgggtgg cgaggtgctg cgcggaggag gcccgtgagc
     8521 gccggaaagc cgggggaacc ggatcgctac gcggtgatcg gccatccggt gtcgcattcg
     8581 ctctcgcccg cgatccacgc catgttcgca gagcagaccg ggcagcatct ggcgtacgca
     8641 aagctgccgg ccccgagaga cggttttgcc cggacggcca gcgacttctt tgccggcggt
     8701 gggagcgggc tcaacgtgac cgtgcctttc aaacaggaag ccgccgggtg ggtcgatctt
     8761 ctggaagggg ccgcccggga ggcgggggcc gtcaacacca tctcccttga atcggggcag
     8821 accaaaggct tcaacaccga tggcctgggt ctcgtcaggg atctcaccgt gaatcacggg
     8881 ctttccatct ccggtgcccg ggtgctcgtg gtcggtgccg gcggcgccac ccagggggtg
     8941 gtgcgtcctc tgatcgccct cggcccccaa gcgctgaccg tcgccaaccg cactgtctcg
     9001 aaagccgctg aactggtcgg ttcgctgggc tctgccgccg gccgcgcaag ggtggcagcc
     9061 tgcgcgccag aggcggcgac gggtccgttc gatctggtta tcaacgccac ctcggcaggt
     9121 ttggaaggtc acggcgaggt cgtgcccgcc gacgcggctg cgggggcagt gtgttacgac
     9181 atggtttaca gcacgggggc gggcgctgcc acggccttct gcgagtgggc gctcggcgtg
     9241 ggggcggttc gcgccatcga cgggctcggc atgctggtag agcaggcggc cgaagccttt
     9301 ctgatctggc gtggggtcag gccggacacc gcgcccgtca tcgacgcgct ccgtcgcgtt
     9361 gcgccgccgg cgtattgaac aggaatggag ctttcggctt gacgatcaag aagcgattca
     9421 ttgcgggggc cgtttgcccc gattgcggcg cgcaggatcg cctggtcgtc gaggaaatgc
     9481 ctgacggtaa gcgccgccgc tgcgtgagct gcggattcac cgacgtcagc gcgtctgagc
     9541 cggaagtggc tttgccgacg cggtttgcgc gaaggggcgt cgattcggag caggcgcgcc
     9601 ccgtgcgtct gctgggcgaa ggcgattcag actcctgatc gccgggcact tttgttcgac
     9661 taaggcgttg aaaacctcac aatttcgact gccttcgttg tgatctggcg gcgcgctgct
     9721 ataatgcgcc cgctcttcgg ttgctctgcc gacgaagagc ttccaggaag caccttcaag
     9781 cccgttttcg ggcacgacca agaggtcacg gtctgaccgc cgtccgcccc ccaattggga
     9841 ggtggacgct ttgactcgac cgggtcgctg gtgcgccgag gcgcaactct ttagcggctc
     9901 ccttatcagg cgaccgacgg tcgccggggg tctttgttgc ttccgaagat gccacaacct
     9961 aacgcaaggg tgagcaggtt tgaaacatta ctggcatggg ctgccgacgt gcctgatggg
    10021 ccttggactg gccgccgctg cgcgagcgga cgagctgaac atgacgccgg gagtgacgca
    10081 gatcagtcgc tccatctacg accttcacat ggtgatcttc tggatctgcg tcgccatcgg
    10141 cgtggtggtt ttcggggtca tgttctggtc gatttttcag cacaggaagt cgcggggcgc
    10201 gcaggcggcc agcttccatg aaagcactcg cctggagatt gcctggacgc tgattccgat
    10261 cctcatcctc atcggcatgg cctttccggc cacccagacc ctcgtcgcca tgtacgacac
    10321 gggcggagag gacatggcgg tcgaggtgcg tggctatcag tggaagtggc agtacaagta
    10381 tctggacgag gactacaaca gcacgttcag cttcttcagc aacctggcaa cctccaggga
    10441 cgagatcgaa aaccgtgtgg tgaagaacga caactacctc ctggaggtcg atgagccgct
    10501 caggatcccg gcgaatcgga aggtgcgctt tctcattacc tcggaggacg tgatccacgc
    10561 gtggtgggtt ccggatttcg gcatcaagcg agacgcgatc ccaggcatgc tcaacgagct
    10621 gtggaccgtc gtggagacgc cgggcgttta ccgggggcaa tgcactgagc tttgcggtat
    10681 ggaccacggc ttcatgccca tcgtcgtcga ggtcatggcg gaggaagact acgacgcctg
    10741 gtatgcggac cgggtgcgca tggaggaagc gcgccacgaa gcgctctcca agacgtttac
    10801 gccggcggag ctcatggagg agggggagcg cgtctacgcg acgttctgcg caagctgtca
    10861 tcaggcaaac ggcaaagggc tgccaccggt gttcccggcc cttgccggaa gccctatcgc
    10921 cacggggccg ctcagcgacc atctggacat catcgtcaac ggccgtgccg ggacgagcat
    10981 gaatgccttt ggcaaccagc tggacgcggc ggagatcgcg gccgtgactc attacgagcg
    11041 gaacgcctgg ggtaacgacg tgggcgacgt tacgcagccc atcgatgtca tcgagttcat
    11101 gtctgggaag gaagccgagg aaacgccatg agtgaagccg tactgactca tcacgacgac
    11161 catcatcacg ggcccgcttc cggaatcacc cgctggctgt tcaccacgaa ccacaaggac
    11221 atcggtaccc tctacctctg gttcagcttc ctgatgctgc tcatcggcgg cgccatggcg
    11281 ctggtgatac gtacggagct gttccagccg gggctgcagt tcgtggaacc cgccttcttc
    11341 aaccagatga ccacgaacca cggcctgatc atggtcttcg gggccatcat gccggccttc
    11401 gtcggtctgg cgaactggct gctgccgatg atggtgggcg cgcccgacat ggcgctgccg
    11461 aggatgaaca acctgtcgtt ctggatcctg ccgttcgcct tcggcatgtt gatctcgacg
    11521 ctgttcatgg aggggggcgg gccgaatttc ggatggacgt tctacgcccc cctgtcgacg
    11581 acctatgcgc cggacacggt gacctatttc attttcggcg ttcacctgat gggtgcgtcc
    11641 tccatcatgg ggagcatcaa catcatcgcc accatcctga acatgcgcgc gccgggcatg
    11701 acgctcatga agatgccgct gttcgtctgg acctggctga ttactgccta tctgctgctg
    11761 gccgtcatgc cggtgctggc cggggcggtc accatgatgc tgttcgacat ccacttcggg
    11821 acgagctttt tcaacgcggc cggcggcggt gaccccgtgt tgttccagca catcttctgg
    11881 ttcttcgggc acccggaggt ctacatcatg atcctgcccg cgttcggcgt cgtctcccag
    11941 atcatcccca cgttcgcgcg caagcctctg ttcgggtacg actcgatggt ctacgccacc
    12001 gccagcatcg cctttctgtc gttcatcgtc tgggctcacc acatgttcac cgtgggcatg
    12061 ccgctggccg ggcagctgtt cttcatgtac gcaaccatgc tcatcgcggt gcccaccggg
    12121 gtaaaagtgt tcaactgggt ttccaccatg tggcgtggat ccatgacgtt cgagacgccc
    12181 atgctgttcg ccatcgcgtt cgtcgtgctg ttcaccatcg gcgggttctc gggcctgatg
    12241 ctcgccatcg cccccgcgga ctatcagtac cacgacacct atttcgtggt ggcgcacttc
    12301 cactacgtgc tcgttccggg cgcgctgttc tccatcatcg ccgccgtcta ctactggctg
    12361 cccaagtggt gcggccggat gtacgacgag accctgggca aggtgcactt ctggctgtcg
    12421 ttcatcgggg tcaacgtgac gttcttccct cagcacttct cgggcctcgc cggcatgccg
    12481 cggcgaatcc ccgactacgc gctgcagttt gcggacttca acatggtctc cagcgtcggg
    12541 gcgttcatct tcggtttctc ccagctggtc ttcctctacg tggtgatcaa agccatcagg
    12601 agcgggcggc cggccaccga ccgagtctgg gagggcgccc atgggctgga atggacgctg
    12661 ccctcgccgg cgccctacca cagcttcacg actccgccca ccgtcaccga ggccgaagcc
    12721 cactcctgat gggggagggg ctaatcgggt aggaggcagc gaagccgcat gcacaagagc
    12781 agcaaaggca tcgtatggaa actcgggctg atggtcgtgg gcatgttcgg cttcggcttc
    12841 gccctggtcc cgctttacga tctgctgtgc gaggtcaccg gcctgggcgg caggaccggt
    12901 gggccctacg cttacgaccc ggcggtcgtg gaggtggatc gctcccgact ggtaaaggtc
    12961 aacctgctga ccaataccaa cgatggcatg ccctggcggt tctggtccga ggtggggggc
    13021 atgcgcgtgc atcctggtga ggccaaggac gtgaagttct acgtcaagaa caccaccgat
    13081 cggcgcatgg tgggccaggc ggtcccctcg gtggtcccga tcacggcaac cgactatttt
    13141 cacaaaactg aatgcttctg tttcgaaagc caggtcttag agcctggcga ggaaatggag
    13201 ctgccgctgc gattcatcgt cgatgcgggg ctgccgaaga acgtgcagac catatcgctc
    13261 agctacgccc tgttcgacgc caccggtttc gcggcggcgg agtaacccga caccgaaaag
    13321 ggagagaacg gccatggcca gctcatcaga ccaagccact tattacatcc cccacagcgg
    13381 gtggtacccg atctgggtgg ccgtcgggtc ggccctgctt ctgttcgggc tgggctcctg
    13441 gctgaacgat ctcaaggccg agacggagcc gagccgcgcg ctgttctata ccggggccgt
    13501 catcctgtcg gtggtgctgt tcgccgggtt tgcaaaggtg gtcaacgaga atctcgaggg
    13561 cctcacgagc ccccagcttc atcgttctta catctggggc atcagctggt tcatcttctc
    13621 cgaggtgatg ttcttcttcg ccttcttcgg cgcgctgctc tatgcccggg tgctctcggt
    13681 tcagtggctc ggcggcgagg gcgcgaaagg attcaccggg gagtacctct ggcccgaatt
    13741 cgaggccgtc tggccggtga tcagcaatcc cgacccggcg gtgttcgaga acccccgggg
    13801 tgatatggcc tcccccgggc tcctgagctg gggcggctac ctgccgttct ggaacacggt
    13861 catcctgctc acctcttccg tcacggtgca tttcgcccac acggcgatca agaacgctga
    13921 ccggaccagg ctgctgaggt ggctcgggct gaccgtcgcg ctgggcgtgg tctttctgtt
    13981 tctgcaggtg gaagagtacg tgcacgctta tcaggagctg ggcctgacgt tgtccagcgg
    14041 gatctacggc tcgaccttct tcctgctgac cggcttccat gggttccacg tcacgctcgg
    14101 cacgttcatg ctcttcgtgc agctggttcg tgccttcagg gggcactttc aacccaagga
    14161 ctgcttcggc ttcgaagcgt cttcctggta ctggcatttc gtggacgttg tctgggtctg
    14221 cctgttcctc ttcgtctacg tattctgaag ccgctcagtc gccggaggtc tgggactgat
    14281 gccacggggc gttgctgtcc atcgagagct ctcccgtggc aaagccgtac aggacggtga
    14341 gtagcagcgc gcccgccagg gttattctta tgcccagggc ataaagcgct cgctttgatt
    14401 ccgggcgttc gctgtccctg aacaggaagg ccaggctgct gaagaggctc agcaccaccc
    14461 ctgccaggag gaagatgatc acgatcttta tcatggagga tgccggcggc gcgagaagct
    14521 gccagagtaa cgcatccatc cggcgggatt aaccgcgttg ggggctgcgc gcagatgggc
    14581 gcccggttgg cgcatgtcgt tggccgtgac gctgctcgtc ccgctgatgc tcgggctggg
    14641 cttttggcag ctcgatcgcg ccgatcagaa gcgcgcacat caggacacgc tgttctcgcg
    14701 ccttgcctca gctcccgtgg cgggccgtga taccctcgcc ccggagccgt tccagcgggt
    14761 tcggctggaa gggcggttcg aggccgatcg ctattttctg gtggataacc aggtggctga
    14821 cggcgtggtg ggctactggg tggtccagag cttttcaact ccgcagggcg tgcgctggct
    14881 ggtcaaccgc ggctggattg ccggcggcga tcgtcgcgac caactgcccc gggtgtcggc
    14941 gcccacgggc acggtggtca cggtgggggt ggcctggccg gacctgggcc tggtcccgct
    15001 tctggacgag gatgactggc ccctggcctg gcccgtccgc gtgcagcggc tcgatgtcgc
    15061 aagaatggct gaacggctgg aggatgccgt gcccgttgaa atccggctgg aaacggccca
    15121 gcccggcgcc ctgcgtgcgg cgccgctgac ggtcggcctc caggcggagc gccacgaagg
    15181 ttacgcggcg cagtggtttg ccctggcagg cgtgctgatc gtcgggtacg cggtctacgg
    15241 gctgcggggc gcgcagcgcg ccaggcgatt gaacggacga acggatgaga gtcgatgacc
    15301 caagctgaag acaaaaccgg ggatcagaga ggccgcgccg ggcgcagcca gctggtggtg
    15361 atcctgctgg tggccggctt ttccatggcg ggggcgtatc tggtcttctt tctcgccagc
    15421 agcggtggcg tctggggcac cacgaacaac ggcgagttcg tgaccccggc gatgaccatg
    15481 gaggccctcg acctgcgcga cgccgcgggc acttccgcga cggcagacgg gcattggtgg
    15541 ctgtggacgg tcgcgcccgg cgggtgcggc gagcagtgcc ggttcgcggt ggagcggctg
    15601 cggcaggtgc acgcgctgct caacaaggat gcatcgaggg tcaggcgcgc gctggtcacg
    15661 tcggcgccgg ggctcgagcc gccgccgggg ctggatgtct ccgatctcga gaggctcgtc
    15721 ggcccggcgg acgggctggc ttccggtatc tacatcgtgg atcctatcgg aaacctcgtg
    15781 ctccgctacc cgctgtccga cgctggcgaa gcggtgctgg aggatctgaa acgactgttg
    15841 aaggtgtctc aaattggctg acaagaccct cctgacgctc tcgaaggccg gcgtggcgct
    15901 ggcgatgctg gtcgtcgtgc tgggagccta cacgcggttg gcggatgcgg gcctgggttg
    15961 cccggactgg cccggctgct atggattcct cacggtgccc gagtcggcgg aagacatcgc
    16021 ccgggccgag accctctttc cggacgcgcc ggtggagcgg gccaaggcct ggtgggagat
    16081 ggttcaccgt tacttcgccg ccggcctcgg gctgctggtc ctggtgatgg ccgggcgcgc
    16141 ctacctgcgt agggagcagg gggggccggt caagctgccg ttggcgctgc tggcgctggt
    16201 catcctgcag ggcgctttcg gtgcctggac cgtgaccatg aagctttggc ctcaggtggt
    16261 gaccgcccac cttcttggcg gcttcaccac cctgagtctc ctgtggctgc tctgtttgcg
    16321 gctcggtgtc ggcagtggct gggaggtgtc cgcaaagctc aggacgccgg ccctgatcgc
    16381 gctgatcgtg ctggtgatgc agatcgcgct cggcggttgg gtgacgtcca actacgccgc
    16441 ccttgcgtgt ccggacttcc ccacctgtca cggcgagtgg ctgccggaaa tggatattgg
    16501 tgaggggttc aacttcgccc agtccgtggg cccgaactat ctgggcggga tgctggagag
    16561 tgaagcccgc gtggcgatcc agctcggcca ccgcctgggt gcattggtcg ccaccctggc
    16621 cgtgggctgg ctgggtttgc tgctgctgcg caccggctcg cccctcggct ggcccgttct
    16681 cgttcttctg gccgtgcagg tggtgctggg aatcctcaac gtgttgctgg cattacccct
    16741 gagcaacgcc acgctgcaca atggtttcgg cgcggtatta ctgctggcct tggttacagt
    16801 aaactatcgg gcctacttgg gggggatgag aagttgagcg aagtcagcct gcaagccgcc
    16861 agctggagcg actaccttga aatgaccaaa ccaagggtcg tcctgctgat gctgctctgc
    16921 gcgctggtcg ggatgttcct ggccgtgccg ggcatggttc ccgtggacgt gctggtattc
    16981 ggcctggtcg gcattgccct ggttgctggc tctgcggcgg tggtcaacca cattgccgac
    17041 gctcagatcg acgcgcgaat ggcgcgcacc caggcgaggc cggtggccac gggcagggtt
    17101 ggtttgctcc agggactgct gttctcagcc gtcctgggcg tgtcgggaat ggtggttctc
    17161 tacttcctcg tcaacccgct cacggcctgg ctgaacctcg cttcctggat cggctacggc
    17221 ctgatctaca ccctttatct caagcgcgcc acaccccaga acatcgtcat cggtggcctg
    17281 ttcggggcgg ccccgcccct gttcggctgg gcggcggtga gcaacagcgt ggagccgggc
    17341 gccctgctgc tggtgctgat catcttcgcc tggaccccgc cccatttctg ggctctggcc
    17401 cttgaccgca aggaggagta cgcctcggtg gacgtgccca tgctcccggt cacccacggg
    17461 gaacgctaca cgaggctgca catcctctat tacaccctga ttctggtcgc cgtcagcctg
    17521 cttccgttcg tgataggcat gagcggctgg ctatacctgg ctggggccac tgccctgggt
    17581 ggcgggttca tctactgggc cgtcgtcctg cttcgcaacc gcaacccgcg ggcaccgctg
    17641 gaaaccttcc gctactccat cgtctacctc ggcctgctct tcgtcgccct gctcgccgat
    17701 cactacgtct tcgccgacca cctggtggga tcgagcctgc agtgagatcc gttacagcga
    17761 cggtgctgct gtgcctgtcg ctggccgcag ccgccgtagg cgccttcgtg tacagcgtcc
    17821 tgcgcgagcc ggttctcagc gatgcggagc ttcgcgatct cggcgtgttc gtgctgcctc
    17881 gcccgcgcgc cattggcgcg tttcagctgg agagcacccg gggcggttcc ttcaccgccg
    17941 ccgaccttca gggcggttgg tcgttcctct ttttcggctt caccaattgt cccgacgtgt
    18001 gcccaaccac gatggccgtg ctggcccagg ctgagcggga gctgcttgca ggctcaccgg
    18061 aggatgccga ccgcttcgcc ggggtgctgg tttcggttga cccggaaagg gacgacgtga
    18121 ggacgctggg tgagtacacg gccgctttct ccccccgctt cttcggtgtg accggacctt
    18181 tcgacgagct cgcctcgttt gcccggcagc tgaacgtggg attcggcaaa gttcccgctc
    18241 cagaagggtc tttcagcgag tatctggtcg atcacggcgc ccagatcgtc atcatcaacc
    18301 cgcagggcca ctatcacggc ttcatccgcg cgccccacac ggcggatacg atcgtgcaga
    18361 cctttgcgtc actggccgcg cggttctgag cgcggcctgc ccgcgtcgcc ggctggcggc
    18421 cggtttccgc cgctctgtcc gtccggggcc ggtgtgatcg gccggctgtt acgcccggcc
    18481 ggtaagctca cccggtgagc ccagccggca agcccgaaac ggcttcaggt gagcagccgt
    18541 tcgtccaggt agaaaaagtg cgcggcgtgc tgctcgacga ctttctcgag ctccaacggc
    18601 gtgttgaagt gcagccctgg ggacacctga cggccctgag cgaacgccag gcgcttgatc
    18661 cagccgttca aagccgcgta cagggtaaaa cccgagaaga accagagctc ggctcgaacg
    18721 agggggcgca gtttctgata cgcccgaagg aagcccagcg ttctctcggg gtcgagcatg
    18781 ccgttggcgt caatgcacca gtcgttggcg gcaaccgcca gatcgtagac gaggaacccg
    18841 ctggacacct ggctgaaccc gcccacgccg ctcagcccgt gctcgttgaa cagcacgttg
    18901 tcacggcaga tgtcaccgtg aatggccccg gcaggcagcc ccgccacgtc ttcccggtcc
    18961 agcatggaga ctacctgctg gacggttgct tccatgagcc tcgcgctcgg atacgagagc
    19021 cggccgtcga gagcggccga cgcctcggcg agccacctct ggtcccaggc ccatgccggc
    19081 agcggtccga ggttgccgcc cgcttcgtga aaacgagcca tgaaccggcc caatgccgct
    19141 acctgccgga cggtagggtt gtaaacgtgg cggccgggca agcgccgggt gaggatcacc
    19201 gtttcgccgt tcaacgtgtc cacgggttgc ccatccagat tcgggatcgc ctttgccacc
    19261 ggaaggcctg cccggtcgca ggcttccaaa gcggcgagct cggcctcgaa tcggggcgcc
    19321 tgcacccgca ataacaggta gtcgtgctcc cttccggccc gccgggttct gacgcgataa
    19381 gcgcaacggc tggcagctga gcctggccag gccgagatga gctcgccgac cccgtagcgc
    19441 gtcagggtct cttcgattcc cgggtggccg ggctcggtga tggcgttcat tggctattcc
    19501 agctgtccag catggccgta tcagaactcg acgatgaccc agctgggcag cagcatgtcg
    19561 gcgcgctcaa gatcgccaaa gcccgtcgtc gagtctcttg gaaccaggta gtagggtttt
    19621 cccaggcggg gaacgatctt gaccattctg agctgtcccg cctggcgaaa ctccaggatg
    19681 gttttgtcct cctgcgcgat gatggtgatg tccgggccct gcagcccgtc ctgcgcttcg
    19741 cttgccagcg cggtgggcgg cagggtcgcg gcagccgcca tcgccagcac tgctgcgcct
    19801 ttgcggcccg tctgtgatag tgtccggagc gagcgttgca cagggggatg ttgaccgaaa
    19861 cggctgtgca aggcaaagca tccgcatcat aaaaaaggtc gacacatggc cagcgacgaa
    19921 accgccgggg cggtaagccc gccgcttctt ctcgtggacg gatcgtccta cctgttccgg
    19981 gcgtaccacg cgctgccgga gttgaaaacg agcaccgggt tgcataccgg cgccgttcgc
    20041 ggcgtcatcg gcatgctgcg ccggctcgcc aaggattacc gggaaagccc catcgccgtg
    20101 gtcttcgacg ccaagggcaa aacgtttcgg gacgacattt atgtccagta caaggccaac
    20161 cggccgccca tgcccgatga tctgagtgag cagatcgagc cgattcatca gatcatccag
    20221 gccatgggcc tgccgctgat cgtggtcccg ggcgtcgagg cagacgacgt gatcggcacc
    20281 cttgctgcgg aggccaccag gaagcggcgc gacaccgtca tttcgaccag cgacaaggac
    20341 atggcccagc tggtgaccga gcacgtgacc ctggtcaaca ccatgagcga gaccaccctg
    20401 gacccggagg gcgtggtcga caagttcggc gtgccgccgg agttgatcat cgactacctg
    20461 gcgctgatgg gggacagcgt ggacaacatt cccggggtgc cgaaggtggg ccccaagacg
    20521 gcggcaaaat ggctcaacga atatggtgac ctggacggcg tcgttgcgag cgccgagcag
    20581 atcaagggca aggtgggcga gagcctgcgc gatcatctcg accagctgcc gctgtcgaag
    20641 gcgctcacca ccatcaaatg cgacgtggct ctggaagtcg gcatcgacga tctcgcccct
    20701 cggcccgtgg acgaagatcg cctcaagggg ctgttcgagc ggctggaatt ccgaagctgg
    20761 ctcgccgagg tgggaggtcc ccaggaggat gcgcctgccc cggcagcggc ctcggccgaa
    20821 gccgaagggg aatacgaatg cgtcagctcc gcggaggcgc tggacgaatg gatcgagcgg
    20881 ctgcgcgcgg ccccgctgtt ttctttcgac accgagacca ccagccttaa ctacatgctc
    20941 gccgagctgg tgggggtgtc gttcagctgc gagccgggca aggccgccta cgtaccgctg
    21001 gcccatgatt atcccggggc gcccgatcag cttggcgtgg cagccgccct tgaccggctc
    21061 aaaccgctgc tcgaggatcc ggcggtcatc aaggtggggc agaacctcaa gtacgacatg
    21121 agcgtgcttc aacgctacga cgtgcagctc gccggagtgg gtttcgacac catgctcgag
    21181 tcctacgtgc tcaacagcac cgcttcccgt cacaacatgg acgacctggc aaagaactat
    21241 ctctcccggg agaccattca ctacgaggac atcgccggca agggcgccaa gcagctgacc
    21301 ttcaatcagg tacccgtgga acaggccgtg tcgtacgccg gggaggatgc ggacatcacg
    21361 ctgcagctgc accagaccct ctggcccagg ctcgagaagg agccgcgtct gacggaggtt
    21421 ttcagggaga tcgagatgcc cctgctgtcc gtcctctccc ggatggaacg caagggcacg
    21481 ctggtggacg gcgccctcct cgaagcccac ggccgcgagc tggccgagcg gatgcggggc
    21541 atcacggagg aggcctggac gctcgccggc gaggaattca acctagactc acccaaacag
    21601 ctgcaggcga ttcttttcga caagctcgag ctgcccgtgc tgaagaagac gccgaaaggt
    21661 cagccctcca ccgccgagcc cgttctggtc gacctggccc gggacttcga cctgccgcgc
    21721 gtcatcctcg agtaccgcag catggccaag ctgaaatcga cctataccga caagctgccg
    21781 ctgcagatca atcagcgcac ggggcggatt cacacctcat atcatcaggc ggtggccgcc
    21841 acgggccggc tttcctcgtc ggatccgaac ctgcagaaca ttccggtgcg tacgcccgaa
    21901 ggccgacgca tccggcaggc gttcgtggcg ccgccgggca aaaggatcgt agccgccgac
    21961 tactctcaga tcgagctgcg catcatggcg catctctcgg ccgacgacgg cctgcgggcg
    22021 gctttcgcgg cgaaccggga catccaccga gccacggccg gtgaagtatt cggcgtcggc
    22081 gtcgatgcgg tcaccgatga tcagcgccgc agcgccaagg ccatcaactt cgggcttatc
    22141 tacgggatgt ccgcctttgg cctcgcgcgc cagctgggca tttcccgaac cctggcccag
    22201 gaatacatcg accgctactt cgagcggtat cccggcgtcc tggcctacat ggacgagacc
    22261 cgggcgcggg cacgcgatgc ggggtacgtg gaaaccgtct tcgggcgccg gctctatctc
    22321 ccggagatca acgcccgcaa catgcagcgg cgtcaggctg cggagcgcgc cgcgatcaac
    22381 gcgcccatgc agggcaccgc ggcagatatc atcaagaagg cgatgctcgc cgtggatgcc
    22441 tggctggcgg gctcggagct ggatgccgtg atgatcatgc aggtccatga cgagctggtg
    22501 ttcgaggttg cggatgctga cctggaccat ctgctggcgg gcgtccgtga tcacatgcag
    22561 ggcgctgccg acctggatgt cgagctggtt gtggattgcg gtaccggggc caactgggac
    22621 gaggcgcact aggctccggc gttgaggtgc tcgcgcagcc aggccaccac cgcgtcgttg
    22681 ccgagcccgc tcgttgcgga gaacaggatg acgtccacca gcggcatggc ttcggccgcg
    22741 cgctgcatgg ctttcagcgc cttggcctga gcgttcctgc ccagcttgtc ggctttgttc
    22801 aagaggacga gcaagggcag ttccgacgcg tccgcccacg ccaggagctc catgtcgaag
    22861 cgctgcccgg gatgccggac gtcgcttacg agcaccacgg cccggagcgc ttcgcggttt
    22921 gacaggtagt cgttcacggt agcctgccat tcgtgctgtg cggaccgggc ggcctttgcg
    22981 taaccgtagc ctggcagatc caccaggcgc ccgccccctt ccacggcaaa gaagttgagc
    23041 agcctggtcc ggcccggcgt cttgcccact tttgccgtgg tgcggcttcc ggtgatccgg
    23101 ttgagcacgc tggacttgcc ggcgttcgag cggccggcga gggcgatctc ggggccgtcg
    23161 ggccggggac atttgcgcag cgacggggcg ctggagatga attgcacggt caggcggtcc
    23221 gcgctgccgc ggtccttgtt ggccttgtgg gtcctttcgg tctccgtcac cctgttgccc
    23281 tggtggtcct tgaagttgca ttctgccagc tcagcggagt tgttatataa tccgcgcgcc
    23341 ccgctcgggg gcgggtagtg taaaggaatc agagccatgc agattcgttg gttgccgtgc
    23401 gcttcggcca tcgttgtttg cctcgggctg tgggcgccgg ggctggcgtg ggccgcgggt
    23461 gatcccgaag caggggctgc tcaggccgtg gtctgtgccg cctgccatgg gcaggacggt
    23521 gcgtcgggtg tggatcccag ctacccgaac ctggccggcc agaacgagaa gtatctatac
    23581 aaccagctcg tcatgatcca gaaagacgac cgcaagatcc ttctcatgac gggccagctc
    23641 atcggcaaga gcgaccagga cctgcaggat ctggcggcat actacgcgtc gttgcccgcc
    23701 aagctgggcc aggctgaggg agacgacgac tccattgcgc tcgccgagca gatctatcgc
    23761 ggcgggatcg ccaagaaagg tgtggcggcc tgcgccgctt gccatgcgcc gagcggcgaa
    23821 ggcaacgcct ctgccggctt tcccgccgtt ggcgggcagg cgggtagtta caccatcgcc
    23881 cagctcacgg cctatcggga aggtcgccga cagaccgacg aggtatacgg cggcatgatg
    23941 cgtggcgtgg ccggcggcct gaccgatggt gagatcgcgg ccctggctga ctatcttcag
    24001 gggctgcact aagcgaaccg gacgttgtcc aacgagtggt tcccaaggcg ccctcggttc
    24061 gaatcgcgca tggcggggac gaggcctacc cgtcgccgca gggtcatggt cggggccgaa
    24121 cagagtcctc cagagaggta gaaatgagag acgtgctcgc tttcctgcta gtggttgccc
    24181 tcggggcaac gggccccgcc tttgcccagg aatggcaaga gggcgttcac tatcagcggc
    24241 tgccggtgag cgtggccacg gcagacgccg ccagggtaga ggtggtggag gtattctcct
    24301 acgcctgcat acactgcaag accttcgacc cggcgttgga agcctggcga gagaaccagc
    24361 ccgaggacgt gggtttcagg cgcgttcccg cgattttcag cgccgagtgg gaaaccctcg
    24421 ccaaagcgtt ctacactgcc cagatcctgg atctcggcgg cgccgtgcac gattccatct
    24481 ttcgggcggt gcacgaagag ggccgggacc tgcgcgaacc tggcgccatt gccgccctgt
    24541 tccagcaagt cgccggcgtg aacccccagc agttcgctca ggtcttcaac tccttcgggg
    24601 ttcgcagccg gatcgagcag gccaaggccc atgcccgggc ttatcgcgtc tccggggtcc
    24661 ccgccctggt ggtggatggc acctatcgcg tggacggccg gatggcgggc agcaacgccg
    24721 ccatgctgga agtggtcaac caccttgtcg caaagcagcg agcggcgcgt ggcatcgccg
    24781 ccccggagcc ggcgtccgtt gaggaagagg acgcggccac ttctggggcc tcgtgatcga
    24841 acgaaatgtt agttagtacg cagtccaatc cgcagcgcaa ggctagactc atgaccaagt
    24901 cgatggagca gtccgccatg ttggcttggt ctaggcgcgg cgagaaccgt agtacggctg
    24961 tcaccccggt cgtgaacatg cacttggagt ccgatgtggc tcccgaggag gcggcgcatt
    25021 cgctcaggct cctcaccttc aacattcagg tgggtattcg gaccaccaag taccgccact
    25081 acgtcaccaa gggctggaag cacgtgctgc ctcacgaagc gcgggccatc aatatccgcc
    25141 acatcgccga tctggtgcgc gactacgatc tggtcgcgct tcaggaaatc gacggcggca
    25201 gccttcgcag cggcttcatc aatcaggtgg agtacattgc cgatcgcgcg cgttttcctt
    25261 actggtacac ccagtgcaac cgtgacctgg gtccgttcgc ccagcacggc aacggggttc
    25321 tgtcgcgaat cgcgcccgtg cacatggaag accacaagct gcccggcgcc attccgggac
    25381 gcggcgcgat cctgatgcgc ttcaagctcg gcgagtcgac ggtctcgctg gtggttctgc
    25441 acctctcttt gggcgggcgt tcccgccagg tgcagctcga gtatgtgcgc gagttgatcg
    25501 acgccgaaga gcacgtggtg gtgatgggag acatgaacag tcatcttgcc cagctgctct
    25561 tcaactcgcc gctttcggaa acggatctgg tgccggcgga tcaggtgcac cccacttatc
    25621 ccgcctggca gcctgcgttt gctctggatc acgtgctggt ctcgccgggg ctgtcgatcg
    25681 acgactacga ggttctggac tgccggctct ccgatcatcg accgatcgcg gtcaccctgc
    25741 gcctcgggga gcccgttacg attcagcagt aggctggcgt gctcgtccgg ccgcattctc
    25801 gcccaagatc ccgttcccgg ctcagcgacc gtttctcgga gcggtttacc gcgcttgccc
    25861 catggcgtag cttccccgct tgcctaagac attgggtacg gtgacggaga tggcggagcg
    25921 taaccccgac gattggctgt cggagcagca ggaatggtgc gacgccattg acgacgtgct
    25981 cgccttcagg ggtgaggacg aggtcaagga gctgcttcgg cggctgcagg cccatgtcag
    26041 tcatcgcggc atgattctga ccgacgccgc gctcaacacc ccgtacaaga acaccatcga
    26101 cgtgcgcgat cagcccgcct acccgggcaa catcgagctc gagatgaaga tcggcgacat
    26161 catctgctgg aacgcggtcg ccatggtgct gcaggcctac gacagcggct cgggggttgg
    26221 tggtcacatc gccacctacc tgtccgccgc gacgatgatg gaagtgggtt tcaatcacgt
    26281 ctttcgaggc gcgtccgaat cctacggcgg cgatcaggtg cactttcagg cccacacctc
    26341 gcccggcgtc tatgccaggg cgtttctgga agggcgtctt tccgagcagg cgttgaccaa
    26401 cttccgccgc gagctggcgc ccggtggtgg tctgccctcc taccctcatc cgcgccgcct
    26461 gccgtggttc tggcagatgc ccacggccag catggggctc tctacgccca gcgccatcta
    26521 ccaggcacgg ttcgccaaat atctggaaag ccgcgggctc aaggccaaca acggcggcaa
    26581 gatctggacg ttcatcgggg acggagagtc cgacgagccg gaggtgctcg gcaccatcaa
    26641 catcgccagc cgggagcggc tggacaacct ggttctggtg atcaattgca atctgcagcg
    26701 gctcgatggc ccggtcaggg gcaacggaaa aatcattcag gagctcgagc gcgcctttcg
    26761 cggcgccgac tggcacgtga tcaaggtgat ctggggcggc ggctgggatg ccattcttgc
    26821 ccgggacgag cacggcattc tccaggagcg tatggagcag gccgtggatg gcgactatca
    26881 gatgtacacg gtgtccagcg gcgacgtggt ccgcgagcac tgggtggaaa catcccccga
    26941 gctcgaggag ctgatgaaga cgctctccga cgaggagatc cggtccatca agcgtggcgg
    27001 ccaggatcac cagaagatct atgccgcgtt tgcccgggcg gccaacgttc gcggcaagcc
    27061 gtgcgtggtg cttctcaaga cggtgaaggg cgacggtctc gggccaggca ccgaaggcag
    27121 caacacggtg caccagaaga agcagctcaa cgagcaggag cgacaggagc tcgcgaagag
    27181 gcttggcatt ccgctcaccc gggaggagat atcgcgagcg gctttctatc gcccggccga
    27241 ggactctgca gagctggtct acctgcgcga ccggcgccat agcctgggcg gttacctgcc
    27301 ggcccgggaa gtgcgctgcg cggcgctgga gcccccgtcg gtggagcttt ttgccgccat
    27361 gctcaaagga tcggacaggc cggtgaccac cacggcggcg gtggtccgta tgctgtcccg
    27421 cctgctgcgt gacccggctc tcggccgata catcgtgccc atcgttcccg acgaggcgcg
    27481 caccttcggg atggacggcc tttttcccca ggcgggcatc tactcgccgg ccggtcagcg
    27541 ctaccagccg gtggatgcgg gcaccatcgc gccgtatcgg gaggccgagg atggtcagat
    27601 tctccaggaa ggaatctgcg aaacgggcgc catggcgtct ttcctggccg cgggcacggc
    27661 ctacgcgaat tatggcctgc cgatgattcc gttctacatc ttctactcga tcttcggttt
    27721 tcagcgtgtc ggcgacatga tctgggccgc gggcgacatg atgtgcaggg gttttctcct
    27781 cggcggcact tcagggcgta ccaccctgaa cggcgagggg gtgcagcatc aggacgggca
    27841 ctcccatctg gttgccaaca cgattcccaa cctgaagagc tacgacccgg cgttcgcctt
    27901 cgagatagcc ttgctggttc gtgagggcat caggcggatg tatcagctgc aggaagacgt
    27961 cttctactat ctcaccctga ccaaccagcc ctaccccatg ccggccatgc ccgaaggcgt
    28021 ggaggacggc gttctcaaag gcatgtactg cttcagccgt gctccggggg cggtggtcaa
    28081 cctgttcggc agcggcgcgc tgatgaccga ggtgttgcgg gcggagagcc ttcttgccga
    28141 gcatggcatt gcgtgtaacg tctggagcgt tacgagctac aacgagctgg cccggcaggc
    28201 gctcgcctcg gagcgagccc gcctgctcgg tgtcggcgac ggcgatgagc gggcctacgc
    28261 cgaagcgctg ctagaggggg aagaagggat attcgtggcc gcctcggact accagaagtc
    28321 ccttcccatg agcatttctc gctgggtgcc gggcccctat accgtgctgg gcacggacgg
    28381 cttcgggctg tcggaggatc gccgggtttt gcgcggctac ttcgaagttt ccgccgagtg
    28441 gatcgcgttc gccgcgttgt cgtccctggc ccagaacaac cggctcgagc gtgacgtggc
    28501 gctgggcttt gcggccaagg cggggcttgc cctggacaag gcgaaccccg ctgagggctg
    28561 aggggcgcgg gatgtgctct ggggcatgcg ccgatggggc cccggattgg cgttgccttt
    28621 gcccgcgcgg gtcgggtaag ttgagaatcg gttcacgtaa aacgagccgc catcgcttca
    28681 aaatccccca agggaccaac tcggctcgaa tcctgtaagt aaggaaaatg gaccttcgtt
    28741 tctcacagtt cgagatcctc acagccgccg gcgcattctt tctttgcctg gcgttcattt
    28801 gcgtgttcaa cgaggcatca gatatccagg acgacgtcgg catacgcgcc cagggcgagg
    28861 cgcgcgagca cggcctttac tgggcgggtg tggaggcctt gggccagcgg gtcaggctga
    28921 cgggtgccgc gccaagcgat gcccaaaggc ttcacgcggc agcagcggtc gccgcgctcc
    28981 caggtgtcac ttcggtccag aacgagatcg aggtgctcgg ccccgacggc gcgtgtcaga
    29041 ggcagatgga ctcggcgctg gcggagcttg gcgtgacctt caagaccggt cgcgccgaga
    29101 tcggcgactc cagctacccg ttgattgccg agcttgcccg gaccatgggc gcatgcgcga
    29161 cgcccatcga gattgccgtg cacacggatt ccctcggcga tgccgccgtc aacctcaagc
    29221 tgagtcagcg gcgagcggac gcggtacgca aggctctggt ccaaagaggc gttcgcccgg
    29281 accgtctcgt agctgccggc tacggcgaga cccagcccgt cgcttccaat gcccgcgaat
    29341 cgggacgtcg ggaaaatcgc cgggttgaat tccgggtggt cggaggcacc gcgtgatcta
    29401 tctcgtcgtc aacatcgtcg gctatctgct ggttgccctg gtcgccggcg tcggtgtggg
    29461 gtggctgctg cacggatccc tcgcaggcaa ggcttccggc gagcgcagag ggctcggttc
    29521 cgtgggcgtc gacgatctcg agctcgcggc tcgcgccgac gcccaggacg agcagctgag
    29581 gaagctggcc gccacgatca ggcagaagga gcgagaggtc gaggcgttga gccgcgagct
    29641 cgaaaggcgg gatcgccgct tcaacgctgc cgacaagctg gcaaagatat cccggcgtct
    29701 tcaacaggaa gccgagcagc tcaaggagca ggtccgcgtt ctcggcgagc agctcgaagc
    29761 cgcgcgggag gaggcccagg gatcccacgg cgcgtcggct gccatggagg aagttctgcg
    29821 cagcgagatc ctcgagctcg agggccggct ccgggagtct tctcaggagc aggaccggct
    29881 cgccaaaacg ctggagcagg aaaaccgcaa ggtcgaggag ctcgagcggc agcgtgatct
    29941 tcagaacagc tctctgcagg ttctgcacaa gcagctggag ctggcccgga agagcaggga
    30001 gggcgctgcc gaggccggtg gttgaggctc agccgaggaa gcgtcggagg atccaggcgg
    30061 ccaccgagag gatggccgag acgatgagca tcgaggtgat gggaaagtaa aaggagagcc
    30121 ccgggcgctc cacccggatg tctcccggca gcctgccgaa ccatccgagc caccccgtct
    30181 gcagcagcaa cccgacgatc acggttaacg ctccggcgag caccagccat ttgcccacct
    30241 cgctcatgct tgcttgtgcc cgatcaggcg accatcgaga ccgcgaatgg tcgtgctggt
    30301 catggctacg ccggcgatgg cgaagccgaa cacgcaacag ccgatcagca ccgccagcgc
    30361 cggcccagcg gcctcggcga gagcgcccat gaacaagcct cccaacggta tcaggctgta
    30421 gccaatcgcg tggatgccca tgacccggcc ccgcaggtcg tcgggaacga cgagctgcag
    30481 gacggccatg gagctgatca tgaagactga ggtgaagacc gatgcgaaaa acgtcgccag
    30541 cagcgcggcg atgaaccaac cacccactgc gaccagagcg aacacggcgg ttagggcggc
    30601 ggacagggtt gcgccgccaa gcatgacgaa cccgagcctc gcatgctcct gcaccccccc
    30661 gaccagcagc gtgccggcaa ccgcgcccac gcctccggcc gagagcagat aaccgtagcc
    30721 ggcctcaccg gactccagga cgtctgcgaa cacgggcatg atctgcacgt agctgcccgc
    30781 gaaaaacatg ccgatgaacg tgagcagcat cagccacttg aacagctcct cgttgacgat
    30841 gaagcgcacg ccctcggcga tctgctccag cggcgagccg tggcgcgcgt cgctggtggt
    30901 cacgtgcagg ccgctgatga cgatcatcat ggtcagaaag ccgccgctgc ccagggcgaa
    30961 cagcacccag gtgtcgctgg ccgctatgat gatcccgccc agtgccggca tggccatgcg
    31021 cgtggcctgc cagatgatgg agttgagcgc cacggcgctc atgaaagccg gccggtcgat
    31081 gagctgtgga aagatggaga ccctggcggg ccagtcgagc ccggtgatga gagagactgc
    31141 ggccgccacg gcgaggacgt gccacacgcg cacggtaccg ctgtagtcca gccaggccag
    31201 cagcgcgagt agcgtggcga tggcccccga tgtccagacc agaatgagac gcttgtcgaa
    31261 gcggtcggcg aacacgccgc cgatcagcgt catcgcgatg ttcggcgcgg cgacggcggc
    31321 gcccagaatt cccagctgca gcgccgaccc tgatagctcg aataccagcc aaccctgggc
    31381 cagggtgatc agctgggtcg cgcccacgga ggcaagagac gccagccagt actttcggta
    31441 gcgcgggaag ccgaaagccg ggtagcgttg cagaaggaag ttggtcatgc catctctctg
    31501 agcggccgtg tagtatgccg tgaccgtatt ggctggcacc actcgccttg ctagaagacg
    31561 tcaccaggtt cggctccaag gcggacgcgc gtcaatgggc gtgggatgcc ctcgacgagc
    31621 gtcgtctggc cagatttccg ttcccacccc atgggcggat tccgaacttc gccggcgcgg
    31681 cggcagccgc gatgcggatt tttgcgcaag ccccctggtc cgaggcgggg gctatcaagg
    31741 tcaatcccga ctcgccccag ctgcccgttc ggttcctggc gctgcagcgt gggatccggg
    31801 tttacgtgcc gacaccgaag ctcgcagcgg gattcattct gctggatccg gcgcggatcc
    31861 cccccgagcg gttccgcgag gcagcggcgc tcaagaccat gctgcgctgg gcgcggccgg
    31921 tagccctcga agacctgccc cagctggatg ccatcgtgac cgggtgcgcg gcggctacct
    31981 cgtcgggcaa gcgggcgggg aaaggcgccg ggtacagcga cctggagttc gccatcctgc
    32041 gggagctcgg ccacgacccc gtcccggtgg ccacgacggt ccacgacgtg cagctggtag
    32101 aggactttcc cattgcccac aacgacctgc ccctaagcct gatctgcacg ccgacgcgca
    32161 cgatccgcgt ccaggcgccg cccgccgcgc ctgccgggat tcaatggggc gagctctccg
    32221 ccgagcagct ggcggccatg ccggttctgg aatccttgcg caggctcaag catggcgggt
    32281 gagcgcaccc ttcccgtgcg cctggcactg gccgtggcgc ttcttgtcag ctcggcttcg
    32341 gccgagcccg ccgccgccgg ggagagcacg gtgatcgtgc tttcctggga cggcatgcgc
    32401 cacgactatc cggacagggg cgactttccc ggcctggccc gggtgatcag cgaaggtgcc
    32461 cgggccgagc ggctcgttac cgtgtttcct tcctctactt ttccgggaca cgtctcttta
    32521 gccacgggca cgtacccgga ccggcacggc atcgtcggca atcacttcgt cgaccggcag
    32581 cgcggcgtct acaggatgga ctccgatgca gactggctgc tggctgagcc catctggatc
    32641 gcggctgagc gccagggggt catggcggcg acctacttct gggtgggctc ggagagcgac
    32701 tggcgcggcc ggggcacccg ttaccgcgag agccccttcg acggttcccg acccgaagct
    32761 ttgaaggtgg atcggattct cgagtggctg cagcttccgg aggggcagcg gccgcgcctc
    32821 atcatgagct actgggctgg cgccgatcgg atcggccacc gtttcggtcc cgacagcgag
    32881 cgcgtcacca cccagatagc cgctcaggat ctgcagctgc agcggctgct ccagggcctc
    32941 gacctgctgg atcgctgggc gtctaccacg ctcctgatcg ttagcgacca cggcatgacc
    33001 gcgagctacc gttacaccga tctgcgcggc atgctggccg ccgccggcat cggcgcctac
    33061 gtcacaggca gcacggtggt gcacgtctac ctcgacgatc cggatgacca tgcccgggca
    33121 ggggaactgc tcggcaagat agagggcgcg cgggcctacc ctggggatgc gctgccgggt
    33181 tggatgcgcc tgtcccacgc ggccagaacg ggcgactggc tggtggtcgc tactcctccc
    33241 ctgaccttca gccggccggc tggttgggag ggagccatgg tggcgaccct gagcatggtg
    33301 ggatggactt tcggcagcca cggctacccg ccggaccatc ccgagatgcc gggcatgttc
    33361 ctggccctcg gccgtggcgt tccggcaggg gcacttctac gggcggtgca tcaggtggat
    33421 ctggctgcca ccatcgccgc gctcctgggc atcgagcccc cgctcgaatc cgagggcacg
    33481 ccggtgccgg gcctggagat tcccgttgcc gcccggcagg cggccaagga ggatgacgat
    33541 ggctaagtgg cagcaacccc gaagcttctg gacgtggggc tatcggtctg atgagccctc
    33601 ggaggaggag cgccatcagg ccgcggctcg ggtatccgaa cggcttggcc gggtggtcga
    33661 gccgcccccc atcccgcgga tcggggaaat tgcgctgcgc aagccgcggc tgtccgttcc
    33721 tgccggcctg cggggttggg tgaccaccga tcatgaggag cgtgtggtgc acacccacgg
    33781 cggtcacccg ctcgagctgc tctctgccgt gcgaggagag ttcgacgcgc cgccggatgc
    33841 ggtagcccat ccgcgcaacg aaacagaact cgaggcgacg ctcgcctggt gcgacgagca
    33901 cggccacgcc gcgattccct ttgggggcgc cacctcagtg gtctggggcg tgcgggtgcc
    33961 cgagagcgcg gatgccgcgg tgaccatcga catggatcac ttccatcagg tgctggagat
    34021 agacgaagtg tcccgggcgg cccgcatcca ggcgggcgtc ctggggcccg atctggagaa
    34081 caccctccgt cctctgggcc tgacgctcag gcatttcccc cagagctttc cctggtccac
    34141 cgtcggtggc tgggtcgcga cccgttccgg cgggcactac gcgaccaacc atacccacat
    34201 cgacgacttc gtcgagagcg ttcgcatgct caccccggcc ggatggtggg aatctcggcg
    34261 tctccccggc agcggcgccg gacccagccc cgatcgcatg gtcatgggct cggaaggcat
    34321 tctcggcgtc atctcggaat gctggctgcg gctgcagaaa cggcccgttt atcgggccac
    34381 ggcgggcgtg gtgttcgaca gctgggaggc gggtgcggaa gccgccaggc agatcgtgca
    34441 ggccaagctc tggcccgcca acctgcgcct gctggatccg gctgaggctg cccagagcgc
    34501 gggcctcggc ggcggccggt ccctgctcat cgtcggtttc gagtccgcgg atttgcctca
    34561 gggcgatctg atgcgcgacg cgttggccat cgcacgggga gcgggcggcc tggtggacga
    34621 cgatgcggtc cgcgtggacg acgggtcggg gaaagccacc ggcagggaag gatccgtggg
    34681 ggcctggcga tcggccttca tcggcgtcaa cgccgggctg accagcgggc tcggtctgct
    34741 cgcggacacc ttcgagacgg ccatcacctg ggaccgctgg cccgagttcg acgcgtcggt
    34801 tcgcgaaagg gtgggtcggg tgctcgagga ggtgctcggg ccgggagcga ggctcagttg
    34861 ccgcttcacc cacgtctacc ccgacggccc cgctccctat tacagctttt ccggcgttgc
    34921 cgggctcggg gcagagcacg agcaatggcg ggtcatcaag gacgaggcca cgtctgcggt
    34981 ggtggacgcc ggcggaacgg tgacgcatca ccacgccgta gggcgcatgc accgtgcggg
    35041 gtgggcggat cagcatccac ctctctatgc ccaggcgctc agagcgatga agcgcaccct
    35101 ggatcccaac gggatcctca atcccggcgt gctcatcgac ccttaggacg gcccacgcct
    35161 gcgactcgct cgtctgctcc cctgttgacg tggctttcac gcgcggccgc gcatagtggc
    35221 cgtcctcttc gttcagggtg ggggcgatgc tggccacgct ttccaggcaa ttggattccg
    35281 aacgggccat ggtgcgccgc ggccgcggct tggactggct gcgcatctgg ccctttatcg
    35341 gccttcacct ggcctgcctg gccgtgttct gggtcggggt gagcccggtg gcggtcgtgg
    35401 tcgccgtggc ctgctacctg atccggatgt ttgcgatcac ggccttctac catcgttact
    35461 ttgcgcacaa ggcgtttcgc acctcgcgcg cctgccagtt cgtgtttgcc gtgctcggcg
    35521 cggcatccac ccagcgggga ccgctgtggt gggcggcgca ccaccgccga catcacatgc
    35581 acacggacac cgcggacgat ccgcaccgcc cacaggacgg cttcctgtgg tcccacatgg
    35641 gctggtttct ctccggcgag cattttcaga ccgacatggc ccgggttcgc gactggagcg
    35701 tcttcccgga gctggtctgg ctcaaccggt tcgacacggt ggtgcccatc ctgctcggca
    35761 cggccatgtt cgctctggga tgggggctcg aacgtctggc acccggcctc ggcaccagcg
    35821 gctggcagat gctgatctgg ggttatttca tatctaccgt cgcgttgatc cacgcgaccc
    35881 tgctggtgaa ctccctggcg caccgctcgg gccgcaggtg cttcgacacg cgggacgaaa
    35941 gccgcaacaa ctggatcgtc gccgttctga ccctcggcga gggctggcac aacaatcatc
    36001 atcgctttgc cggcgctgcc cgacagggat tctactggtg gcagctcgac gtctctttct
    36061 acctgctgaa gctcatgtcc tggctcggct tgatctggga tctcaagccg gtccctggct
    36121 cggtgctccg ggaggggcgc tcgtgagaat cgcggtggtg ggagcgggcg tatccggtat
    36181 cgccgccgcc tatcggctgc agcaggaggc cgacgtcgtc ctcttcgagg ggcaggggcg
    36241 gctgggcggt cacaccgaca cccacgccgt gctcgtcggc ggtgccaccc acgccgtgga
    36301 ctccggattc atcgtgttca acccggacaa ttacccgcgt ttctcgtcgt ggctggacga
    36361 gctgggggtt gaatctcagc cgtcggacat gtccttcggc gtctcgaacc aggctaccgg
    36421 cgtcgagtac ggcagccgcg gactgggcgc tttgttctgt cagcggcgca acctggtctc
    36481 accccggttc ctgcgcatgc tggccgatct tcgcaggttc taccgtgacg ccgtcgatta
    36541 tcccctggac gagtcgctca ccctcggcga gctgctcgac cgtcacggct acggcgccgg
    36601 gttcgtcgag gatcatatcg ggcccatgtg cggtgcgctc tggtccgctg cgccggacgc
    36661 ggctctggcg ataccggccc gccacgtgat cgccttcatg acgcatcacc gcatgctgca
    36721 gctggacggt cgcccgcagt ggcgggtgat caagggcggg tcgtccacct atctgcaggc
    36781 gtttctggcc cgtttcagcg gcgaggtgcg gctggccgac ccggtgcagg cggtagccag
    36841 gacggcgtcg ggcgtggaca tcacctcggc gtcgggaacc gcgcgcttcg atgccgtcgt
    36901 gctggcctgc cacagcgacg aggccctggg tctgctggcc gaccccagcg aggcggagcg
    36961 ggacgtgctg ggcgccatcg gctaccagcc gaaccgggtc gtcgttcact ccgacgaccg
    37021 ggtcatgccc gagcatcggc cggcgtggtc gagctggaac gccttggtcg gggccgagcg
    37081 ccccgagcgt tgccaggtga gctactggat gaacctgctc cagtcattgc ccgggcgcga
    37141 aaactttttt gtgacgttga acccgcagat gcccctcaag cgcgtgtgga gcgtgcgcga
    37201 gtacgctcac ccggttttca ctcgccgtgc ccgtgcagcc cagcgtcgca aggccgagat
    37261 cagcggcgtt cgcaatacct ggttctgcgg tgcttactgg ggctggggct ttcacgagga
    37321 cggcttcgtc agcggcatgg aggttgccga aggcatgctt gggtctatgg ccgatgccgc
    37381 ctgagctcct ccgcgatagc gtctgtgagg ggtgggttta ccaccgccgc gccgccccgg
    37441 aacaccgttt ccggtaccgc gtctggatgc tctggctcga tgttgcccgc ctcggccagg
    37501 ggcccttgcg cctgctgcgg cgccggctgg gactgagccc gctcgggata gcgcccaagg
    37561 actttctcga cggcagcgac gaccttctcg gcgcggttcg cgcgcgcctg gccagtcacg
    37621 gctacccggc gccggcccgg gtgtttctgc tcacccagcc ccgcagctgg atttccgggt
    37681 tcaatccggt cagcttctac ttctgcgtgg gccaagcagg gcttgattgc attcttgccg
    37741 agatcaacaa cacgccctgg gatgaacgcc atacctacgt gctggacgcg cgcggccagt
    37801 cgggcgagtt ctccttctct tttcccaagg cctttcacgt ctcgcctttc atgccgatgg
    37861 atctcgaata ccgttggcgg ttcgccatcg agcccgaagc ggtgaagatc gccatgggtc
    37921 tgttcaggga aggggaagag accttcaacg ccgggctgag gctgtcggta gtgcccatga
    37981 cccggcgccg cctgctcctg ggcatggccc gctatccgct gcagaacgtg gcgacgctgg
    38041 cacgaatcta ctggcaggcc ctccggctct attccaagcg cagccgtttc tatccgcacc
    38101 ccgatcgcgc tgaggaggcg ccggcatcat gaccgacaac accttgtatg gcgacggccg
    38161 acgaaccgcg ccctcgggcc cgtttgccgc gctggctcgc aaggcgctgg gcaggttgcg
    38221 agatggcacc atccggctga ccgaggcggg ccgtaccgag gttttcggtt gcggcgcccc
    38281 ggaggtgcac gtggaggtcc gtgatccgcg tttcttccgg cgcctggtca tgggcggaag
    38341 cgtcggggcg ggcgagtcat atatggacgc ggactggtcc tgcgacgatc tggtgtctct
    38401 gatcagaatc tttgcccgca accgcgacgc cctggatgtg gtggatggcg ccggcagcgt
    38461 tcttgtgcga tgggccaacc gctccaggca cctgctgagg cgcaacaccc gacggggaag
    38521 ccggcgcaac atcgaagcgc actatgacct gagcaacgag ttcttcgaga cttttctcga
    38581 cggcaggatg atgtactcgt cggccgtgtt cgagtcgccg gatgcaaccc tggaggaggc
    38641 gagcgaagcc aagctggagc ggctgtgccg aaaactcgag ctcggcccgg aggatcacct
    38701 tctggagatc ggctcgggct ggggcgggct cgccgtgtac gcggcagcgc agttcggctg
    38761 tcgggtcacc acggtcacga tttcccccgc tcagctcgaa gccacccaag cgcgggttcg
    38821 ggccgccggg ctccagggcc gcgtcagggc cgttctgtcg gactatcgcg atctggacgg
    38881 ctgttacgac aaggtggtat ccgttgagat ggtagaggcg gttgggcacg agttcctcga
    38941 tcgctacttc gagtcgctgt ccggactcac ccgaccaggt gggctcatcg ccctgcaggc
    39001 aatcaccatt gcagaccggc ggtaccgcgc agcgctcgca gaagcagact tcatcaagaa
    39061 acacatcttc cccggcagct tcattccctc ggtctgcgtg ctggtggacg cggcggcgcg
    39121 ccggaccgac gcggtgctgg tcaacctcga ggacatcggc ctcgactacg cgcgcaccct
    39181 gcgcgcctgg cgtgagcgcc tggaagcccg gcgcggggag attcagtcgc tgggcttcga
    39241 cgagcggttt ctgcgcatgt ggcggtttta cctcgcttat tgcgaaggcg ggttcctgga
    39301 gcgggccatc agcgacgtac agcttctttt cgcaaagccc gcgtaccggg ggcagccctg
    39361 gcggggtgcc ctcgacggtt gagggggcgg ctcgaatcaa caaggagacg gtttcatgag
    39421 catactcatc gatctggccg aggcgcgccg atttccggac gccctggttc gcgcgggtat
    39481 ccgcaggctc gtcgcgcagc ggctgaaaga cgaggggtcg caaggcgtcg ccgctcaggc
    39541 ggagcgacac cagcgcctgc tgcgcgagct ccaggaaagc cccgttgcgc tggatacgga
    39601 gctggccaac gagcagcact acgaggtccc cgccgagttc tttcgtgcgg tgcttggaaa
    39661 gcacctcaag tacagcagct gttactggga acgcggcgct gccgacctcg acgaggcgga
    39721 ggcgcacatg ctggcgctct acggcgagcg ggcggagctt gccgacggcc agagggttct
    39781 cgatctgggc tgcggctggg gttccttcac gctctgggcc gccgagcgtt ttccggggag
    39841 ccgcttcaca gccgtcagca attcaaatgg ccagcgcgcc tacatcgagt cacaggcggc
    39901 cgctcgcggg ctgaaaaacg tcgaggtcat cacggccgac gtcaacgatc tgcggctgcc
    39961 cgggatctac gaccgtatcg tatccatcga gatgttcgag cacatgcgca actacgcgtt
    40021 gctgctgaat cgtatcgccg gctggcttgc gtccgacgga aagctgttcg tccacatctt
    40081 ctgccaccgc cacctccttt atccctacga gaccgagggg gacggcaact ggatgggccg
    40141 ttactttttt accagcgggc tcatgccggc agcggacacg ctgctgttct ttcaggacga
    40201 tctgcggtgc gagcggcggt ggagcgtttc cggagtgaac tacgagaaaa cggcccgcgc
    40261 ctggctggac aatctcgatg ccaacgaaag ccgagtagtg caggctctcg aaggaaccta
    40321 cggggctgcc gaggtcgccc gatgggttca gcgctggcgc atgtttttca tggcctgcga
    40381 ggagctcttt gcctatcgtg gcggcgagga gtggctcgtg tgccattacc gttttgcccc
    40441 acgctgacgg ggcggcgctg cgcccgccgg agaacgggtt gaaatgaaac ccaagggaat
    40501 cagatccaag ctcgtccggg ccttcttcat tcaggtgacg atcatcagct gcgtcaccct
    40561 ggtgggcata gccgtggcgt acaacatcat ctacggcgtc atgctccggg aagccctcaa
    40621 cgacgaggcc gcgcacttct ggagccgtta cgaatcggat ccgagtcaca gcctgccgga
    40681 cgtatacaac ctgcagggtt tcatggccgt ggacggggac ttctcgggcg tgccggagca
    40741 ataccggggc ctcgctccgg tgggcttcag ccggcagcca tacgaggacg cccaggcact
    40801 ggtgcacgta tcggaacgtg acggcgagcg gctctttctg gtgttcgagt acgatcaggt
    40861 gtccgccctg gcctttttct tcggcatcgt cccgctcgcg ttggtcctgt tgctgattta
    40921 cggcacgtcc ttcgtcacct accgactctc ccagcgggcg gtgtcaccca tcgtgcgcct
    40981 ggcggcctat ctggaggact tcgacttcaa tgccaatctc gagatcagtc gcgacctcga
    41041 accgctgcgc gcccatgccg acgcggaggt cgtgagcatg attcaggcgg tggagcattt
    41101 caccacccgg ctggcagcct tcgtcgaacg tgagcggatt tttacccgcg acgccggcca
    41161 cgagctgcgc acgccggtgg cgatattcaa aggcagcctg gatctgctcg agcagagcgg
    41221 ggatcgaccg gctcacgagc acaaggcgct tgcccgaatg agacgcacgg tctcggacat
    41281 ggaatcgctg ctggagaccc tgctgctgct ggccagagag gagcaggtag agatgcctcg
    41341 ggaggacgtg agcgtcaacg ccctggtggc gggccagctc gagctgctgc agcccatggc
    41401 cgagcgcgcc gagaacagcc tggcgctgca cgagcgcgct gagctgcgcg tgcgcgcccc
    41461 ggagaaagtg ctcgagatcg tgcttggcaa cctgatccga aacgccatca actacaccca
    41521 cggcggccgc gtcgatgtga cggtaaccga ccgctccgtc caggtgggcg acacgggcgt
    41581 cgggatgtcg ggtgaggagt tgcagaacgc gttcgagccg ttctaccggg tggacgagag
    41641 cagaggggcg atccgaggcc acggcctggg cctagccatc gtgaagcggt tggcccacca
    41701 ggccggctgg agcatctcgg cccacagcaa gcctggtgaa gggacccgtg tagaagtggc
    41761 cttcttcccc gacgactagg tcaggggatg ggcaggtagc cgccgtcgat gcctttggtg
    41821 acgatgctca ccgcgtcgtg ggggtgaagg ctttcgtggt gctcgacgcg cagccagaag
    41881 tcattgaagg acggatcgtc gttgagcgcg gcctgcagct tgcgcgcggc atcttcgcag
    41941 aacatcagat tctgaccgtt gcgcagggcg aattcctgct cgtcctggcg tttcacggcg
    42001 gcctgaaccg gcgtgccgat ggcggtctcc agcgtgtcga tgagggccgc gatggggagc
    42061 tcggtcacgt cgtcggccag ctgggtgatg acctttgcca cgctcctctg gctgtgaggc
    42121 gtggcgacga tgccctgctc cgtgccgagc cactcgttga cgtcggcgaa gtcgatgggc
    42181 cggccgctgt cgaagcggtc ggcaaacgcc tgctggatca gctgcctggc cagcgcggcc
    42241 gagcaagggc aggtgctcga gtagagaatc tccaccccca gctccagggt catcttgccg
    42301 tcggccaggg tgcccttcac ggtgatgggg taggagctcc agccggcatt gtccgaaagc
    42361 agcgccatct ggcgcagctc gtactcgaag tcgaactgca cgaacgcccg gctgctcagg
    42421 tcgagatgag agctgtgcat ggagccgagc agcattttca gccccgcgac ggacagggga
    42481 cgggtgcggg cgtgctcgtc gagcagaaga tacagccggg acatgtggat gcccttggca
    42541 tgggggtcgt tcaggtccac gtacacctgg accctggcct gaacgcgttg gttgacgttg
    42601 ccggcccgga tcaggatggg ctgatggatg tcgctcatgc ccacccagtc cagggtgcct
    42661 tgcggcttgg catggggtcg tccggcgatg tcgggcatgg aggccagctc gacgggcttt
    42721 ggtgtgctca tgggttatcc cctcgatcaa tgatctgatg agatcaagct agaggggcgc
    42781 cggtgaaagg ggtgtgaaag gcgttgcccg tcagccggcg tctggctcag gaacccgcac
    42841 gcggtatccg cggcctgcca gagtctcgat gagcggctcg ttgaacggcc ggtcgacggc
    42901 cttgcgcagg ttgtagaggt ggctgcgcag ggtgtcgctg tcgggaaggt cgtctcccca
    42961 caattcccgc tcgatggtct cgcgtgggac caccttcggg tgctcgcgca gcaggatctt
    43021 gaggatgtcg aacagcgtac gcgacagggg gatctcggtg tcgccccgga acacggacat
    43081 ggtgtccaga tccatgctca gctcgcccac gttgtaggct gcgctcaccc cgcggccgcg
    43141 gcggatgagc gcctcgatgc gcgcgaccag ctccggcatg tcgaagggtt tgaccagata
    43201 gtcgtccgcg ccgacgtcga agccgtcgag cttgtcctga agctggtccc gggcggtgag
    43261 catgatgatg ggggtggaga gcctggcatc cctgcgcagg cgccggcaga cctctatgcc
    43321 gtcgatgccg ggcagcatga tgtccagcac gatggcgtcg aaggcttcgc tggtcgcgag
    43381 ctgcacggcg agcagcccgt ccgcggcgta gtccaccacg tagttctttg cctccaggaa
    43441 cgcgccgacc gtctctgcca ggtccgcgtt gtcttccacg aggaggatac tgccttcggg
    43501 tcgactcatc ggcgcatctt agaggcaggg tcacgccggc cgcaacgccc tcgaagcccc
    43561 ttggtttcgg ctcgcggcaa tgggcacaat gatccgtccc cccgacatgg gcgctcgcat
    43621 ggcgtcgagc cgatagatcc atggatgcga ccggtttcat attgcaggcc acctaccgca
    43681 ttcacgcggg ggtgcccgtg atttacctgt tcggccgcct ggagtccggc gaaacgtttc
    43741 tggtgcgtga ccatcggcag cggcctcatt tctacgtgcg gcaggaggat gcccggcgag
    43801 ccgaggcctt agacggcatc gccctcgagg cgtccggcgc cagaacgttc tccggggacg
    43861 cggtgtgtcg ggtgaccgtg cccaccccct cagatgcgcc gcccgcgcgc gagcggcttc
    43921 acggccttgg ggtcgacacc ttcgaggcgg acgtccgctt cgccatgcgc tatctgatcg
    43981 accggggcat ccgcggcggc tgccgcatcc gcggggcggc aagccgcgga gaaggggtag
    44041 gctgggtgtt cgacgatcct gaggtcggcg ccgccgaagc cggtatcgag ccggtggttc
    44101 tttccttcga tatcgagacc gacgccaagg cggaccggct gctcggcatc gccgtctacg
    44161 gcgccggcgt ggacgaggtc ctcatcgtcg accccgagcg acggcccatg cccgagcgtg
    44221 cccggggctt gccctccgag cgggcggtgc tggaagcttt ctgtcagctc gttcgcgacg
    44281 tcgacccgga cgtgctgacg ggctggaacg tggtcgattt cgacctgacg gtgctcggga
    44341 gggttgccgc cagagtgcgc catccgtttc agctcggtcg gcaggcgggc gcgctccgca
    44401 tccgccccgc ccagggttac ttcgggagcg gcaacgcgac cattcccggc cggctggtgc
    44461 tcgacggcat ggacctgctt cgcggggcgt tcgtgcggat ggaagaatat tcgctggacg
    44521 ccgtggcgcg ggaggtcctc ggcgagggca aaacgctgga tggcgacgtg cgcgaccgtg
    44581 cgggggagat catgcagcgc taccgccagg atctgccggg gtttgcgcgc tatgcgcgaa
    44641 cggatgcgcg cctggcgctg gacatcgtca ctcgcctcga tctcatgaac ctggctttcg
    44701 ctcgcagccg gctgacgggc atgacgccgg atcgggtggc tgccagcatc gcctctttcg
    44761 acttccttta cctgtccgct ctgggcaagc ggggcctggt tgcgcccacg gtgcgttccg
    44821 atgacagccg cgtccacgct gcgcagtcgg gaggccaggt tttcgagccc gtgaccggcg
    44881 tgcatcgcaa cgtcatggtc tttgatttca aaagcctcta cccgagcatc atccgaactt
    44941 tcaacgtcga tcctctgggg ttcgtgccat cgggcgacgc ggagcctgac gtgatccggc
    45001 tcgttaacgg cgctggattc cggcgggatc cggccatcct gccgcgcatg ctcgatgacc
    45061 tgtttccgcg ccgggaggct gcgaagacag ctggggacag cgttgcctca caggcgatca
    45121 agatcctcat gaactctttc tacggtgtgc taggcacccc ggcctgtcgg tttcacaacg
    45181 tcgagatcgc aaacgccatc accggtcagg gccgccatct gctgcagtgg tcgaaacgct
    45241 ggttcgagac gaggggcttc gacgtgctct acggcgacac cgacagcctt ttcgtgcggt
    45301 cgcggcaggg cgacgaggat gctgcccttg cggaagcgag ggatctcgcc gcgcggctca
    45361 atgcggacat tgccgccgag gtgctcgagc gctggggggt cgtcagccgc ctcgagctgg
    45421 aatttgaaaa gctctacgcc aagctcttcc tgccgtctgt tcgtcacggt gcggcgggcg
    45481 cgcgaaagcg ctatgcgggg ttgcctgtcg gggccgaaga ggtggagttc gtgggcatgg
    45541 aggtcgtccg ccgggactgg accgagcttg ccaagcgtgt tcagcgggag ctgtatgcca
    45601 ggctcttctc cggagccgcc gtggatgact acctggccgg agtcgtcgcg cagctgcgtc
    45661 gcggcgagct ggacgagctt ctggtctatc gcaaagggct caggaagcga ctggacagct
    45721 acacggcgag caaccctccc cacgtggctg cggcgcgaaa gtcgggagag ccgcctgggc
    45781 gcaccgtggc ttaccttgtg accagcgcgg gccccgagcc tctcgacgcg ctgcagcatg
    45841 agcccgatcg ggagcattac gtcgacaagc agataaagcc ggtggccgag cccgttctcg
    45901 gggcgctggg tctggaattc gcccgggtga tcggcgacga ccggcagatg gggctgttct
    45961 gaaggggagg gctcccgccc gccggcacct ggtcagagct ggttcagcac ccagccgatc
    46021 agcagaacct ggggaacgca gatggcgggc agcatcaggg caatcttcgt cgatccggtg
    46081 gcttcgcgca ggaccatgat ctcggtgtag tcgctggcca cgccggcgag caggaaggta
    46141 aagctgtttc ccggggcccc cgctcggttc atcagatccg cggcgatggg cacggagccc
    46201 tcggagcaga cctcgaagat ggtcgcgccg acgagggtga ggcccaggcc tagcaggctt
    46261 ggtccgaacc actgcgcgaa gtcctcgggg ctgagaaagg ccctcaacgc ggcagcgacg
    46321 actagcccga acatcagcca gcggagcacg atacgggtcc cgcgcagtcc gttgatgacg
    46381 atgtccgcga gcaggcgagg ctcccactcg atggtgctga ccagctcacg ggaagccgga
    46441 ccgaaacgga agttcaccgg aagatcgacc tgggcgtcgt tagccgggat tcgtcccgtg
    46501 cgggccaggc ggtcgaacgc taggccggtg gtgatggcaa ccaccagcga caggccgatg
    46561 aaggcgatgg tccagccgag cccgatgagg ccgacgagga tgaaggtcag agaaacggag
    46621 ttccacgggc tggcgatgag aaatgccatg acctgcccgg cgcttgcgcc ccgctggtag
    46681 agcttggccc cgacggcgag gatcccgtgg ctgcacaggt cgagcagcac gccggcgaca
    46741 gcggcccgca gaacgccgtt ccggcttccg ggaggtccga gcagcgcgat gatgaactcc
    46801 tggggtatcc gctccaggac gccgatgatg aggatggcca gcgcgatgcc ccaccacatg
    46861 gccgcgagca tctcgtgcac ggtgtgagct gcatggccga gcggggtccc catgggcgcg
    46921 gcgcccgtca gcgccaggat cagaagaacc agcgcgatgg ccgcgcaggt catcagcagg
    46981 taatcggtgc ggtatctggc cagccggctg gcgggcgtcg gcacgtcagc cacttgggcg
    47041 tcccatcgag cgaagagggt tgaggctacg cctggtcctg tcctgctggc aagtgaggtt
    47101 gctcgacaag tcgaggcgcc tacttcacga agcccagctc cgcgaggtcc ctgacaaacc
    47161 agggggtgaa gcgagcgggc caccgttcga tttccgcggc gagctcctga aggccgacgg
    47221 cgcggacctc ggcgacctcg gcggcatccg gctcgaaggg gccgtcgtgc acgccggtga
    47281 acgcctgctg gagctcgttg tcccgcaagt tcagctccgc ctgatgtgac cgccaccgcc
    47341 gcacgtcgcc aatcggctcg agcgaaacgc cccttacgcc gagctcctcc tctaggccgc
    47401 gccgggcggc ggccagatat gtctcgccgg gcaccaggtg ctcgccgacg ctgtagtccc
    47461 acagatcggg atagaggtcc ttgcccggcg cgcggcgttg gatgagcagc gcgccggtgg
    47521 tgtcgaaaag aaagacgtgg gcagagcggt gccaggcacc ggtagcgtgg acccgatggc
    47581 gtgccatgag ccccatggcc ctgcccgcct cgtcataggt ttcgaacagc tcgtccaatg
    47641 gtccttcgac tctgctgggg atggcggacg ctatactttg gcctttgcac gtcgaggtca
    47701 atgatgtccc agcgacgcag gcggccggcg ggtccaggac agcggtgctg aagctgcagg
    47761 gaatccgtct ggccgagggc gccgcgcccc tggaccatga gttccggtcg gggcagatta
    47821 ccgtggtcct cggccgcaac cgctcgggca agacgcgcct gtgccgattg ctggccggcc
    47881 tggagcccac gggtgagggc cggatattgc tggatggggt cgacgtctcg aatatcccgg
    47941 cgggccaacg gccggtctct ttcgtgtatc agtcgttcgt caattacccc accctcaccg
    48001 tggcggagaa catcgcctcg cccatgcggg cccggggctt gccgtcgcgt gagcgtcgcc
    48061 ggcgggtcga ggacctggcg gcaaggctgc acatttcgga gctgctcgac cggcgcccgg
    48121 cccagctgtc cggtggccag cagcagcgtc tggcgatcgc ccgggcgctg gccaaacagg
    48181 cgcgcgtcct ggtcatggac gagccgctgg tgaaccttga tttcaagctt cgtgaggcgt
    48241 tggagctgga gctgcgcggg ctgctccggg atgccggtat cgccgtggtc tatacgagct
    48301 cggacccccg ggacgctttc aacctgggcg acgaggtgct cctgcttgcc gaccacgcac
    48361 ccgtgcaggc cggccggccg ctggacgtct acgagcgtcc ggtcagcgtc gctgccgcgg
    48421 acctgatgag cgaccccggt gtcaatctgc tgcccgggcc tgaccggccg gcggcgattc
    48481 gtcccgagca tctgcacgtg agccgccacg caagggatga cgagacgttt gctgccgagc
    48541 tcctcaccct cgagaccaac gggtcggaga cctatctcca ctgccgggtg gcggacgcgc
    48601 actgggtggt gaagctggag ggcctgatcg atctgaagct ccggccgggg gatacgctgc
    48661 cgcttttcgc cgagcagcgc tccctgctgc gattctcgga ggcgatgacc gatgtccagg
    48721 attgagctgg acggcgtcag ccaccgatat ccggaggcgc ccagcgaaac gtttgccgag
    48781 ctcgagctgg tcatcggctc cggcgaagcc cacgcgctgc tgggcagctc gggcgcgggc
    48841 aagacgacgc tgctcaacct cctctccggc atcgagcagc cgacgaccgg gcgtatcacc
    48901 ttcgatggcc gggacgtctc gcgcaccccg ccgcgcgctc gcaacgtggc ccaggtgttc
    48961 cagtttccgg tgctttacga atctctcagc gtggcggaga acctggaatt tcccctgaaa
    49021 acccgtggcg tgcgcgccgc cgagcggcgt cgccgcgcga aagccatggc ggagcgcctg
    49081 ggcatcgcgg agctgatggg ccgccggccc ggcaagctgt ccctgttcga aaggcaactc
    49141 gtggccatcg gtcgcgcgct ggtcaggccc gagttgtccc tcgtgctgct cgacgagccg
    49201 ctgacggcgg tggagccttc gatcaagtgg cagctcaggc gggagctgaa ggccgtgcag
    49261 caggagatcg gcgccaccat gatctacgtg actcacgacc agaccgaggc gcttaccttt
    49321 gccgaccgca tcagccttct gcaccgcggg cggatcctgc agaccggctc ggcccgtacc
    49381 ctgttcagcg cgcccgctca cgagcaggtg ggacacttca tcggctcgcc ggggatgaac
    49441 ctcgttcccg gcgagatcgc cgagggcgcg ttgcggctcg gagggcgcgt ggtggccccg
    49501 gcggcgggtg cggagcctgg accgtgcacc atcggcttcc gcccggagtg ggcatcggtg
    49561 gcgccgtccg ggcctgacgg atcgggcctg ctcgggacga tcaccgcggc ccgggtgctc
    49621 ggcgtgcgac gtggggtgcc cgtcgggatc ctcagcgtcg acctcggcgt aggcgttgtg
    49681 caggttcgcc aggctttgtc cgcttccccg ggggagcgtg tgggtgtgaa cgtggacgcc
    49741 gggcaggtcg tcgccttccg ggaaggctgg cgcctcccgg gaatcggtgc cgagaccgga
    49801 cggggggcag gttcgtgagc ggacgtcggc ccgacaaccg ggcctggctc ggcgttctcc
    49861 cggtggtgct ggtggtcgcg ttcagcgccg tgattcccct catgacggtg gtcaactact
    49921 cccttcagga catcttcgat cagcacacgc gtctgttcgt cggcttcgac tggtatcgag
    49981 agatccttcg cgacccgagg ttcctcgagg cgctgttgcg acaacttgcg ttcagcggcg
    50041 cggtgctcct catcgaggtt ccgctcgggg tcatggtggc acgcagcctt ccgcgcagcg
    50101 gctggctcgc gccggcgctc gtcgtcctga tcgctttgcc gctgctggtc ccttggaacg
    50161 tcgtcggaac catctggcag atctttgcgc gcccggacat cggcctgctc ggcgccgcgc
    50221 tgaccggcct gggtttcgag ttcaactaca cgtccagccc cgtcgacgcc tggatcacgc
    50281 ttctgctcat ggacgtctgg cactggacgt ccctggtggc gctcctggcc tatgccggtc
    50341 ttgcgtccat ccaggaggcc cattatcagg cggccgagat agatggggcc ggccgctggc
    50401 aggtcttccg gctggtcgag ctgccgaggc ttaggcccgt tctgaccatt gccgtgctgc
    50461 tgcggctgat ggacagcttc atgatctaca cggagccttt cgtgctgacc ggcggcggcc
    50521 cggggaacgc caccactttc ctcagccagt acctgaccag catggcggta ggccagttcg
    50581 atctggggcc ggcggccgct ttctcggttc tctacttcct cgtcaccctg ctgatgtgct
    50641 ggctgttcta taccagcgtg atcaggaagg aagacgaatg acccgccgca gccactggtt
    50701 gatcttcata tactgcgcgt atcttctgct gcccgtttac tggcttgtca acatggcgct
    50761 gcgcagcaac cgggacatcg tgggcgggct ggagatcgtt cccggccggc ctacgctggc
    50821 caacttcgcg gagatcgtga ccaacccggt ctggtacgaa gggtttgcca actcgcttac
    50881 gtacgtcggg gtgaacacgg tgatttctct cttggtggcg ctgccagccg cattcgcttt
    50941 ctcgcgcttc cggtttctgg gcgacaggca tcttttcttc tggctgctca ccaaccggat
    51001 ggctccgcct gccgtctttc tgctgccgtt ctttcagctc tattccgccg tcgggctctt
    51061 cgacacgccg ctggcagtgg cgctcgccca ctgcctcttc acggtgcctc tggcggtgtg
    51121 gattctggaa ggtttcatgt cggcggtccc ccgcagcctg gacgagatgg cgcgcatcga
    51181 tggccacagc ctcccctact tcttcttcag aatttttctg ccggtcatcc gaggcggcgt
    51241 tggcgtgacg gtgttcttct gcttcatgtt cagctgggtc gagctgctgc tggcaagaac
    51301 gctcacagcg agcgcggcga aacccatcgt cgtggtcatg acccgaacgg tcagcgcttc
    51361 cggcatggac tggggcgtgc tggcggccgc tggcgtgctg accatcgtcc cgggcgttgc
    51421 cgtggtgtgg tttgtcagaa ggcatctcgt gaagggcttc gccctggggc gggtgtgaaa
    51481 gcgggggccg cagggcaatg agtttttcct ggatggcgtg gacgctcccc accgcgatct
    51541 tctttggcgc gattctctgc ctgctcgtgg tcatgacggt gctcgagatt cgatggccga
    51601 cgaggacgcg ccggggtctt ctgccgctgg caaccacgcg cggtgatcgt ctgttcatcg
    51661 gtttgctggt ggcggccttc gttcacatcc tctggctggc cgcgctcgac gcgcccgtgc
    51721 tcttggcgac cggcatcagc ctggggcttc tcgcgctgct catccgctgg ggctgattgg
    51781 tcgagcacgg ttcctggcgt ttcctgctaa ggtatgcgcc gttcggccag gggggatgcg
    51841 gatgcggggg atcgttcttt ttcttggggc ggcgactttg ctgctcgccg gttgcgatgg
    51901 gtccgcgccg gacgcgcccg gggttgaccg gcacgcggcg gcgaagcgct ggataaccca
    51961 ggagtttcaa ccctccacgc tcacgccaga cgagcaggcg gcggaaatgg cgtggttcat
    52021 tgaggctgcc gcgccgttca ggggcatgga aatcagcgtc gtctcggaaa ccctgaccac
    52081 tcacgagtat gaagcggggg tgctcgctcg ggccttcgcc gagatcacgg gtatccgggt
    52141 gtcccacgat ctcattcagg aaggcgacgt catcgagaag ctgcagaccc agatgcagtc
    52201 cggccgcaac gtctacgacg cctacgtcaa cgactccgac ctcatcggca cccacgcgcg
    52261 ttacggttac gtggtcgcgc tgtccgactt catggccggc gagggcgccg acgtcaccct
    52321 tcccaccctg gacgtcgacg atttcatcgg ccggagcttt accacgggtc cggatggcaa
    52381 gctctaccag ctgccggacc agcagttcgc caacctctac tggtttcgat acgactggtt
    52441 ccagcgcgag gatcttcgtg cccgattccg cgagctctac ggctacgacc tcggcgtgcc
    52501 cgtgaactgg tcggcatacg aggacatcgc ggagttcttc tccgaatacg tccggaccat
    52561 cgacggccag cggatctacg gccacatgga ttacgggaag aaggacccgt cgctgggctg
    52621 gcgcttcacc gatgcctggc tgtccatggc cggtgccgga gacccgggga tcccgaacgg
    52681 cctgcccgtg gacgaatggg gaatccgggt ggacggctgc cgtccggtcg gcgcctccgt
    52741 cagtcgcggc ggggcggcca acgggcccgc ggccgtctac gcgctggaga agtacatcga
    52801 ttggctgaag cgcttcgcgc ccccgcaggc cccggggatg actttctccg aagccgggcc
    52861 ggtgccggcc cagggtcaca tcgcccagca gattttctgg tacacgacct tcaccgcgga
    52921 catgatcgac ggcgacctgg ccgtggtcga cgaagacggc ctgccgcgat ggcgaatggc
    52981 gccgtccccc catggacctt actgggagga aggcatgaag ctcggttatc aggatgccgg
    53041 cgcctggacg ctgcttgcca gcacgccgct ggagcgtagg aaggctgcct ggctgtacgc
    53101 gcagttcgtg gtttccaaga ccgtctccct caagaaaacc ctggtggggc tgacgcccat
    53161 tcgtgattcc gacctgcggt cggcggccat gaccgaggcc gcgccgcgtc tgggcgggct
    53221 ggtggagttc tacaggagcc cggcgcggac gctctggacg cccacgggga cgaacgtgcc
    53281 cgactatccg aagctggcac agctgtggtg gcccaacgtg gccaatgcgg tgagcggcgc
    53341 cgtcagcgct caggaggcca tggataccct ggcgcgggat caggacagag tgctggagcg
    53401 ccttcagcgg gcgggcatcc agggcgattg cggaccggag ctgaatccgg aggtggatcc
    53461 cgaagagtgg ctcgcacggc ccggtgcgcc caaggcccgc ctggccgacg agaagccgcc
    53521 cggcaagacg gttccctacg atgagctggt ggcagactgg ggggctttgc cgcggccggg
    53581 cgagccgatc ggccgaagga gtcactgaaa catggcacaa ccaacacaga ctgccagggg
    53641 cggcccgggc cggggcagcg cgtttggctg gatctggatc gcctatctgg cagctgtcgt
    53701 ggtgggtgtg gccggcacga tgatcgttgc cgacccggcc accgatcacc ccgtttatct
    53761 ggccggcatt gccgacgttc tggccaccgt cgtgatcttc gcattcagcg tcgctttccg
    53821 aaattccagt ttctacgatc cgtactggag cgtggtgcca ccggtgctgg gcctttactg
    53881 gtggtgggtt gcgggctccg tagaagatcc gcgggtctgg cttgccatgc tcgtcgtcag
    53941 ctgctgggcg gtacgcctga cctacaactg ggctttcggc tggacgggcc ttggccacga
    54001 agactggcgg tatcgcgacc tgcagaac
//
LOCUS       DBNHLM010000250         7528 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_993166, whole genome shotgun sequence.
ACCESSION   DBNHLM010000250 DBNHLM010000000
VERSION     DBNHLM010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7528)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7528)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7528
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_993166"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="AC16QF_11710"
     CDS             <1..226
                     /locus_tag="AC16QF_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449268.1"
                     /translation="YRRLRARRAELRAMGELPSGWELARTLTSYSERGADYVRTLHSI
                     MKANNLDAVDDAYLRDMEPIALVPVGDGAH"
     gene            complement(267..1121)
                     /locus_tag="AC16QF_11715"
     CDS             complement(267..1121)
                     /locus_tag="AC16QF_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6449269.1"
                     /translation="MSLFRIALRVIVLLALPVWAVAGTLDEIAASGEFRLAYRADTPP
                     FSSRGKGGEPEGFTIDLCRIIARDVQAALNLDEMKITWVPVTAENRFDAIVSGRAHLE
                     CGSSTVTLSRQERVDFSNLTYVTGASLMRWADSDIDRVGDLDGRRVSAIAGTTTEKVL
                     KETLERDGIDAKVVVVGDHAEALQLLLDRKIDAMAGDQATLFGIGFKTQGDDDLVITE
                     DLLSFEPYALPLRRNDADFRLAVNRSLSELYVRGDVGRSWEKWFGSHGVRPTRLLLML
                     YRLNSFSE"
     gene            complement(1207..2763)
                     /locus_tag="AC16QF_11720"
     CDS             complement(1207..2763)
                     /locus_tag="AC16QF_11720"
                     /inference="COORDINATES: protein motif:HMM:NF015746.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HIR6449270.1"
                     /translation="MREPHSSASGRGPAAMGRFLDAVERAGNRLPDPALLFFLLLLAV
                     WALSALLSQLDYGHVDPRSGDPVEVRNLLTGEALTSFFAGMVETFVGFHPLGVVLLAM
                     LGIGVADHTGFIKAGLRRLLSLTSQRLLTPMLFLIGILSHSAVDAGYVLVIPLGGVIF
                     QAAGRHPLAGIAVAFAGVSGGFSANFLPSALDPMLQGISQAGAQLMDPGLVLNPLNNW
                     FFTSASTLLVVLLGWYLTARIVEPRLADEPLDGDLADAPALDDLSDEERRGLRAGLVA
                     MLLGIALLAVTAWPDSSAWRSGGQLTAAGAPLMQSIVPLIFLLFIIPGVVYGYASGTV
                     RGHRDVIAGMSQAMSGMGYYIVMAFFASLFIAEFGRSNLGVLVALQGAAWLEAAAMPA
                     AVTVVGVVAITGVVNLFVGSASAKWALLAPVLVPMLMALGISPDLAQAAYRVGDSSTN
                     IITPLMPYFPLVVVFAQRYVTRTGIGTIVAMMLPYSVGFLVVWTLFLLVYWMLGIPLG
                     LQASYGYPGG"
     gene            complement(2839..3279)
                     /locus_tag="AC16QF_11725"
     CDS             complement(2839..3279)
                     /locus_tag="AC16QF_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449271.1"
                     /translation="MAATVIARIAGAYLLSVLVAYLLAATAATQASLAGLEGLGVTIT
                     VGERISTTGQDLMGMLPTYGLLIAIGFAIALPIAAAFARFIPSWRAVGLVAAGAAAVG
                     LIHVGLNSVFETHLVAATRTTVGLLVQVMAGGVGGYAFYRLRDP"
     gene            3418..4449
                     /locus_tag="AC16QF_11730"
     CDS             3418..4449
                     /locus_tag="AC16QF_11730"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR6449272.1"
                     /translation="MSADYRLVEVAEGLELPWCVAFLPDGRLLVTERPGRLRQISPEG
                     EIGSPMEGIPEVLFRAQGGLFDVAPHPDFAENRLIYISYAHGTPRANTTRIARGRLDG
                     NSLKDLEVIFTARPNKTTPQHYGGKFLFLPDGTLLFTTGDGFDHREEAQDLSGLLGKT
                     VRIRDDGSIPDGNPYVRREGADPAVWTWGHRNPQGLAVDHVTGRVFLHEHGPRGGDEI
                     NLLRPGRNYGWPVITFGLDYSGARISPYQQMPGMEQPLHSWVPSIAPSGLAVYRGDVF
                     PEWQGDLFVGALVDREARRVRLRDDRVVEEEALFGELGERLRDIRVGPEGHLYLVTDE
                     GRVLRVERP"
     gene            complement(4487..4972)
                     /locus_tag="AC16QF_11735"
     CDS             complement(4487..4972)
                     /locus_tag="AC16QF_11735"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012075279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4865..4867),aa:Sec)
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIR6449273.1"
                     /translation="MSTFHDIRMESIDGEPVELSKFQNNLCLIVNVASRUGLTPQYAG
                     LRTLQEQYGDRGFTVLAFPCNQFGAQEPGSNEEILDFASSKFDVNFPMFGKIEVNGSG
                     ACELYQYLKAARADEEGNADIAWNFTKFLVDGDGNVIARFAPPVTPEEIGEQIGSLLP
                     G"
     gene            complement(5036..5479)
                     /locus_tag="AC16QF_11740"
     CDS             complement(5036..5479)
                     /locus_tag="AC16QF_11740"
                     /inference="COORDINATES: protein motif:HMM:NF015180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HIR6449274.1"
                     /translation="MIVYATMPNAPSPLPEAHPAVQPVPNGMDRFAITLSGLCLLHCL
                     LIPVVLAMAPAFSSLVLGTETTVHWVFLGMAVPTSVWALWRGYGRCQHLRALVLGIAG
                     LLVMFLGVSHLFDPRLEVPLTLVGVTMVVVAHVLNLRIPEPAPRG"
     gene            5610..6038
                     /locus_tag="AC16QF_11745"
     CDS             5610..6038
                     /locus_tag="AC16QF_11745"
                     /inference="COORDINATES: protein motif:HMM:NF013487.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6449275.1"
                     /translation="MRGRDWLIGLVALGAASAATASEGELEYREHTMEAIGGHMQAAV
                     DILRQKVPHASHMTIHADALAALSGIVGTLFPEGSEGGDALPAIWEEPEDFAGRVEAM
                     REAATAFSAAARSNDDAALGQAFQALGQSCKGCHDNYRAD"
     gene            complement(6042..6644)
                     /locus_tag="AC16QF_11750"
     CDS             complement(6042..6644)
                     /locus_tag="AC16QF_11750"
                     /inference="COORDINATES: protein motif:HMM:NF013460.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR6449276.1"
                     /translation="MRIWHWVLAACVTGSLYTGLAGEIALMERHQQFGYGVLGLLLFR
                     LGWGLWGGRYARFGNYRLAPRRLIEHFRGLDHGAAHTAPGMAFTIVFLLALAAQVGTG
                     LFATDDIFTQGPLSRLVENDTARALTGIHHRVYWLILAALAAHLTAHAVYALRRDPMP
                     LAMITGRKRLDLEPARAAWTAALLTALGAAALVWAMLELA"
     gene            6775..>7528
                     /locus_tag="AC16QF_11755"
     CDS             6775..>7528
                     /locus_tag="AC16QF_11755"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6449277.1"
                     /translation="MALALEEHTATREGHTTFYLAAGPEDGPLIIFVHGWPELSLSWR
                     HQLPALAALGYRAVAPDMRGYGRSSTYERYEDYAQRAVVGDMMALLEGLGRTEAIWVG
                     HDWGAPTVWSIASHHPEACTAVAGLCVPYFTLERGLQACVDQVNRDVYGPEYPAGQWE
                     YQLFYQEHFDRARAEMDANPLNTVKLLFRRGDPNGAGRPAATAMTRINGGWFGPAGEA
                     PDVPRDDDVISEEDLAVYADALARNGFFGPNAY"
BASE COUNT         1462 a         2475 c         2467 g         1124 t
ORIGIN      
        1 ctaccggcgc ctgcgcgcac gccgagcgga actgcgtgcc atgggtgagc ttcccagcgg
       61 ctgggagctg gcccgcaccc tgacctcata ctcggaacgg ggagcggact acgtccgtac
      121 cctgcactcg atcatgaagg cgaacaacct ggacgccgtg gacgatgcct acctgcgcga
      181 catggagccc atagcgctgg ttcccgtggg cgacggggcg cactgagcag cccagcgggc
      241 ggccgcctga cgagccgtcg cgcgccctac tccgagaagg agttgaggcg gtagagcatc
      301 agcagtagcc gcgtcgggcg caccccgtgg gagccgaacc acttctccca ggaccgaccc
      361 acgtcacctc gaacgtagag ctcggagagc gagcggttga ccgccaggcg aaagtcggcg
      421 tcgttgcgcc tgagcggcag agcgtatggc tcaaaggaga gaagatcctc ggtgatcacc
      481 aggtcgtcat caccctgggt cttgaatccg atgccgaaga gcgtggcctg atcgccagcc
      541 atggcatcga tctttctgtc cagcagcagc tgcagcgctt ccgcgtgatc acccacgacc
      601 accaccttgg catcaatccc atctcgctcg agggtttcct tgaggacctt ctccgtggtc
      661 gtgcccgcga tggctgaaac gcgccgaccg tccagatcac cgacccgatc tatgtccgag
      721 tccgcccagc gcatgaggct ggcgccggtc acataggtca ggttggagaa gtcgacgcgc
      781 tcctgccggg acagggtgac cgtggaggag ccgcactcca gatgcgcccg gccggagacg
      841 atggcatcga atcggttctc cgccgtcaca ggcacccagg tgattttcat ctcgtcgagg
      901 ttcagcgctg cctggacgtc acgggcaatg atgcggcaca ggtcgatggt gaagccctcg
      961 ggctcgccgc ccttgccgcg cgacgaaaac ggcggcgtgt ccgctcggta ggcaagccgg
     1021 aactcgcccg aggcggctat ctcgtccagg gttcccgcca cggcccaaac cggcaaggcc
     1081 agcaacacga ttacccgcaa cgcgatgcga aataagctca tgagaagacc ccgattccaa
     1141 tgattctgat cagccaccta ctctagccga tcacggccgg cgggtcccct acggcgacga
     1201 tcgggctcaa cccccgggat agccgtagct cgcctgcaag cccagcggaa ttccaagcat
     1261 ccagtaaacc agcaggaaaa gcgtccacac cacgaggaag cccactgagt aaggcagcat
     1321 catcgccacg atggtgccga tgcccgtgcg cgtcacgtag cgctgcgcga agaccaccac
     1381 caggggaaag tagggcatca gcggcgtgat gatgttcgtc gaggagtcgc ctactcggta
     1441 ggcggcctgg gcgagatccg gcgagatgcc cagagccatc agcatcggaa ccagcactgg
     1501 cgcgagtagc gcccacttcg ctgaggccga gccgacgaag aggttcacca caccggtgat
     1561 ggccaccacc cccaccacgg tgactgccgc cggcatggcg gccgcctcga gccaggccgc
     1621 tccctgcagc gcgacgagca cgcccaggtt ggaccgtccg aactcggcga tgaacagcga
     1681 agcgaagaag gccatcacga tgtagtaacc catcccgctc atggcctggc tcatgcccgc
     1741 gatcacgtcc cgatgccccc gaaccgtgcc ggaggcgtaa ccgtagacca cgccgggaat
     1801 gatgaagagc agaaagatga gcggaacgat gctctgcatg aggggcgcac ccgcggcggt
     1861 cagctggccg cccgagcgcc atgccgaact gtccggccag gccgtgaccg ccagcagcgc
     1921 aattccgagc agcatggcga caagccccgc gcgtagcccg cgacgctctt cgtcggaaag
     1981 atcatccagg gcgggcgcat cggccagatc gccgtccagg ggctcgtcag ccagccgcgg
     2041 ctccacgatc cgagccgtca ggtaccatcc gagaagcacg acgagcagcg tggaggcgga
     2101 ggtgaagaac cagttgttga gcgggttcag caccaggccc ggatccatca gctgcgcgcc
     2161 ggcctggctg atgccctgca gcatgggatc taaggcggag ggcagaaagt tcgcgctgaa
     2221 ccctccggag acgccggcga aggccaccgc gattcccgcc agagggtgtc ttccagccgc
     2281 ctgaaagatg acgccgccca ggggtatgac gagcacgtat ccggcatcca cggcgctgtg
     2341 gctgagaatg ccgatcagaa agagcatcgg ggtgagcagc ctctgcgaag tcaggctgag
     2401 cagccggcgc agccccgcct tgatgaagcc ggtgtggtcg gccaccccta taccgagcat
     2461 ggcgaggaga acgacgccca gcgggtgaaa ccccacgaac gtctcgacca tgccggcgaa
     2521 gaagctggtc agggcctcgc cggtcagcag gttgcgaacc tccaccggat ccccgctgcg
     2581 agggtcgacg tgaccgtagt cgagctgcga gagcagcgcc gatagcgccc agaccgccag
     2641 caggagcaag aagaacagga gcgccgggtc gggtagacgg ttgccggcgc gctcgaccgc
     2701 atccaggaac cgacccatgg cggccggccc gcgaccgctg gctgagctgt gcggctccct
     2761 catgaatccc gcgctcccct ggtccttctg ctcttctcag gagtcgtcgg ctgcacgagc
     2821 cgactttgcc tgcctcagtc acgggtcccg cagccgatag aaagcgtagc cgccgacgcc
     2881 acccgccatg acctgaacca gaaggcccac cgtcgtccgg gtcgcggcaa cgaggtgggt
     2941 ctcgaacacc gagttcagcc cgacatgaat gaggccgacc gcggccgctc ctgccgcgac
     3001 gagcccgacg gcccgccagg aaggaatgaa ccgggcgaag gcggcggcaa tgggaagcgc
     3061 gatggcaaaa ccgatggcaa tcagcagccc gtaagtgggc aacatcccca tcaggtcctg
     3121 gcccgtggtg gatatccgct cacccaccgt gatggtcacg cccagtcctt ccaggccagc
     3181 cagggaggcc tgggtagcgg ccgtggcggc caggaggtag gcgaccagca cggataggag
     3241 ataagcgcct gcgattctgg cgatcacggt cgcggccaat ggtttcacct ctctgagcag
     3301 atgtcctagc atagcggctt cgcttcaccc ttcgaaccct cattcaatcc ggaagtcgcc
     3361 ataaagcctt caacgctaat ctttctgacg ctgtctctgg ccatctccac cgaggcaatg
     3421 tcggccgact accgcctggt cgaggtcgcc gagggcctgg agctgccgtg gtgcgtggcc
     3481 ttccttcccg acgggcggtt gctggtcacg gagcgtcccg gtcgccttcg gcagatctcg
     3541 cccgagggcg agatcggctc gcccatggaa ggaatccccg aggtgttgtt ccgcgcccag
     3601 ggcggcctgt tcgatgtggc cccccacccg gacttcgctg aaaaccggct gatctacatc
     3661 tcctatgccc acggcacgcc gcgagccaac accacgcgca tcgcccgcgg ccgcctggat
     3721 ggcaactcgc tgaaggactt agaggtgatc tttacggccc gcccgaacaa gacgacgccc
     3781 cagcactacg gcggcaagtt cctgttcctt cccgacggga cgttgctttt caccaccggg
     3841 gacggcttcg accaccggga ggaagcccag gatctttccg ggctgctcgg caaaaccgtt
     3901 cgcatcaggg atgacggaag cattcccgat ggcaatccct acgtgagacg cgagggcgcg
     3961 gacccggcgg tgtggacctg ggggcaccgc aacccgcagg gcctggccgt ggatcacgtg
     4021 accggccggg tatttctcca cgagcacggt cctcgcggcg gggacgagat caatctcctc
     4081 aggcccggcc ggaactacgg ctggccggtc atcacgttcg gcctcgacta cagcggcgcg
     4141 cgcatctctc cttaccagca gatgcccggc atggagcagc cgctgcactc ctgggtacct
     4201 tccatcgcgc cgtcaggcct cgccgtctac cggggcgacg tctttcccga gtggcagggc
     4261 gacctgttcg tcggcgcgct ggtggacagg gaagcgcggc gagtgcgcct gcgcgacgat
     4321 cgcgtcgtgg aggaggaggc cctgttcggg gaactgggcg agcggctgcg ggacatccgg
     4381 gtgggcccgg agggtcacct ctacctggtc accgacgaag ggcgggtgct gcgcgtggag
     4441 cggccctgag aaatctgggc cccgcacggt ccggggcccg agcatgctag cccggcagca
     4501 gcgacccgat ctgctcgccg atctcctccg gcgtcacggg cggcgcgaaa cgggcgatga
     4561 cgttcccgtc cccgtccacg agaaacttcg tgaagttcca ggcgatgtcc gcattgcctt
     4621 cctcgtctgc acgggcggct ttgagatatt ggtacagctc gcacgcgccc gagccgttga
     4681 cctcgatctt gccgaacatc gggaagttca cgtcgaactt cgagctcgca aaatccagga
     4741 tctcctcgtt ggagcctggc tcctgggcac cgaactggtt acacgggaag gcgagcacgg
     4801 taaaaccccg gtcgccgtat tgttcctgaa gggtacgcag acctgcgtac tgaggggtga
     4861 ggcctcagcg gctcgcgacg ttgactatca ggcagagatt gttctgaaac ttggacagct
     4921 ccacgggctc gccgtcgatg ctttccattc gaatgtcatg aaacgtggac atgagcgttc
     4981 tcctccctat gctcgatccg aaccgaccga tcatagtcga ttcggcgaaa ccaagtcacc
     5041 cgcgcggagc cggctcggga attcgcaggt tgagcacgtg agccaccacc accatggtga
     5101 cgccgacaag ggtgagcgga acctcgagcc gcggatcgaa aaggtgagaa acgcccagga
     5161 acatcacgag caggccggcg atgcccagca ccagggctcg caggtgctgg catcttccgt
     5221 agccgcgcca gagggcccag acgctggtcg gcacggccat ccccagaaaa acccagtgca
     5281 cggtcgtctc cgtgccgagc acgaggctgg aaaatgcagg cgccatggcc agcaccaccg
     5341 gaatcaacag gcagtggagg aggcagagcc cggacagcgt gatggcaaag cggtccatgc
     5401 cgttgggcac gggctgcacc gctggatgcg cttccggcag aggtgacggg gcattaggca
     5461 tggtggcgta aactatcacc gggccggccc gctgaacact gccaaagaag ggaccggatc
     5521 tgcaccgctc ccggacggcg gcaattgccg cggagccgga tttcaaatac ggtgcgcgca
     5581 atcaactcca gcaacgatga ggagagttca tgcgaggtcg agactggctg atcgggttag
     5641 tggcgctggg tgccgccagc gcggccaccg caagcgaagg ggagctcgaa taccgcgagc
     5701 acaccatgga ggccatcggc gggcacatgc aggcagccgt ggacatcttg aggcagaagg
     5761 tgccgcacgc ctcccacatg accatccacg ccgatgcgct ggctgcgctg tccggcatcg
     5821 tcggcacgct gttccccgaa ggatccgagg gcggcgacgc cctgccggcg atctgggaag
     5881 aacccgagga ttttgccggg cgggtggaag ccatgcgcga agcggccacg gccttcagcg
     5941 ctgcagcacg atccaacgac gatgcggcgc tcggccaggc ctttcaggcc ctgggtcagt
     6001 cctgcaaagg ctgccacgac aactaccgcg ccgactgacg ctcaggcgag ctccagcatc
     6061 gcccagacca gagcagcggc tcccaatgcg gtgagcaacg ccgccgtcca ggccgcacgc
     6121 gccggctcca ggtcgaggcg tttgcgaccg gtgatcatgg ccagcggcat cgggtcacgc
     6181 cgcagcgcgt aaacggcgtg agctgtcagg tgggcggcca gggccgcgag gatcagccag
     6241 tacacccggt ggtggatgcc cgtgagcgcg cgggccgtat cgttctcgac gaggcggctc
     6301 aacgggcctt gggtgaagat gtcgtcagtg gcaaacaaac ccgtgccgac ctgcgcggcc
     6361 agcgcgagca ggaacacgat ggtgaaggcc atcccgggcg cggtgtgcgc agcgccgtgg
     6421 tcgaggccgc gaaagtgctc aatcaggcgt cgaggggcca ggcggtagtt gccgaaacgc
     6481 gcatatcgac ctccccacaa gccccagccc agccgaaaga gcagcaggcc gagaacgccg
     6541 tagccgaact gctggtggcg ttccatgaga gcaatctcac ccgcaagccc ggtgtagaga
     6601 gagcccgtta cgcaggctgc caatacccag tgccagatcc tcatgggcag ttcccagatc
     6661 agggtgcccg cagaggcgcc cgcgcccgtc ggcgaaatcc tggtaggtgc cccgtttgcc
     6721 ataagggata tgattcgaac aatcacgatg aggtcaagac acgggagaag ggctttggca
     6781 ctggcactgg aggagcacac cgcaacacgc gagggccaca cgacattcta tctcgccgcg
     6841 ggccccgagg acggcccgct gatcatattc gttcatggct ggcccgagct ctccctgagc
     6901 tggcggcatc agctacccgc gctggccgcg ctcggttatc gagccgtcgc ccccgacatg
     6961 agagggtacg gaagatcctc gacctacgag cgatacgagg actacgctca gcgcgcggtg
     7021 gtgggcgaca tgatggcgct gctggagggc ttgggacgca cggaagcgat ctgggtaggt
     7081 cacgactggg gggcgccgac tgtgtggagc atagcgagcc atcatcccga ggcctgcacc
     7141 gccgtggccg ggctctgcgt gccctacttc accctggaga ggggattaca ggcgtgcgtg
     7201 gaccaagtga accgggacgt ctacgggccc gagtacccgg cggggcaatg ggaataccag
     7261 ttgttctatc aggagcactt cgacagggcc agggccgaaa tggatgccaa ccccctgaac
     7321 accgtgaagc tcctgtttcg caggggcgac cccaacggcg ccggccggcc ggcggcgacg
     7381 gcgatgaccc gaatcaacgg cggctggttc ggtcctgccg gagaagcgcc cgacgtgccc
     7441 agggacgatg acgtgatcag cgaagaagac ctggcggtct acgccgacgc cctcgcccgc
     7501 aacggcttct tcggcccgaa cgcctact
//
LOCUS       DBNHLM010000251         6997 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_574554, whole genome shotgun sequence.
ACCESSION   DBNHLM010000251 DBNHLM010000000
VERSION     DBNHLM010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6997)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_574554"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..915)
                     /locus_tag="AC16QF_11760"
     CDS             complement(115..915)
                     /locus_tag="AC16QF_11760"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6449278.1"
                     /translation="MKRQLPFELIYQSSALVLAVILVHLVYVTVIRPNADAIIAAQAE
                     RAAAGEAYAQERSLYVVLKDYEQESCFVLMLWAMAIMGYKTRSALRERSLLQEDIVKI
                     SEGMSVLPEDTREYARAVQALPDENRNYLLPRALMAALQRFASTRNVQDVSEAVRSVC
                     ESESDRLDSELSMVRYIAWAIPSIGFIGTVRGISEALGQAYEAVEGNIAGVTTSLGVA
                     FNSTFTALVISIVIMFLMHQLQLIQERLVLDAQNYCDERLLRHLQVRD"
     gene            complement(937..1143)
                     /locus_tag="AC16QF_11765"
     CDS             complement(937..1143)
                     /locus_tag="AC16QF_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449279.1"
                     /translation="MKGPLLFAAAALLASTMGAGFAVAAEEAADTRDRAGEEEEADGE
                     PSPEIFVPSEEISEDFAVSFPVDI"
     gene            complement(1140..3230)
                     /locus_tag="AC16QF_11770"
     CDS             complement(1140..3230)
                     /locus_tag="AC16QF_11770"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR6449280.1"
                     /translation="MSTRNGQDNNASQVQADDPEIIEAVSYTPREQAPKRKLPLTPFQ
                     IGAVLVAIPVMAIMWFLFTAKSVRLEFDPPAASESVSEGPAIALGDIYLMLDGEYRVS
                     ARAEGYEPLDELLVVGDARSQTHRFEFTKLPGRVTFLSNPDGAEVIVAGALLGRTPLD
                     GVKVPAGPATISMKLDRYQRLDMEAEIEGLDRAQTVEGSLLPDWAEVSVSSIPAGAEI
                     LLNDQPTGRLTPAVIEVPTGEHEIRLKAPGHRGHRQRILVAALDEVTLPEVTLLRADG
                     LLQVTSRPAGAGVTLNGQFQGETPVEIAVKSGISYRVKIFKAGFAPGERSVRVASGGE
                     RTVDLTLTRLTGQLIVTTQPAEAELFVDGRNLGNANQTLTLPTVTHRVEIALDGYAGY
                     STEITPRNGVTQEIKVRLLTLEEARLAAMKPIIQTSQGQELVLLNPGPFTMGASRREP
                     GRRANETLREVGMERIFYLSRTEVTNAQFLDFAPGHDSGEFQDHELNKDDQPVVGVSW
                     QEAALYCNWLSAREGLPPFYATDLGKVVATNPAATGYRLPTEAEWEWAARQNGPDSTG
                     YRFPWGTALPPPDRHGNYADRSAAHLVGRVVFGYNDNHIVSAETGTFAPNAIGIYDLA
                     GNVAEWVNDFYEIPGAEPTKDPLGPDTGEYRVIRGSSWMNGTITDLRLSFRDYGVDGR
                     EDVGFRIARFAEPS"
     gene            complement(3238..4749)
                     /locus_tag="AC16QF_11775"
     CDS             complement(3238..4749)
                     /locus_tag="AC16QF_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449281.1"
                     /translation="MSEDFGEIRPKSVPLTHSAPERPSASTRPSLRPLAAGASVLLVM
                     ATAFVVFVVVPDLVQPPTLPGTPATDTAGATSDSPRPAARDEKLPPFQALMKEQARAR
                     AQEELARFVELQLDLEQTMQVGQWGQAEYDAAKRLATAGDEQFLGESFEAAIESYAKA
                     ATALAALIEDGKARFASAMRDGRTALERRDEKNAAGAFARALTISPDDQEAQQGARRA
                     ALLPEVNTAMREAKNHELAAEYRDALAVYERVRAMDPKTAGLEAGIRAARAGIQAETV
                     KGHLSSGFAALEAGRLDAARAAFGQALKLDPGNPVALGGLEQVDDASAGRRLATLRGR
                     AEAAEAAEDWETALQSYVAVLESDGNIQFARDGRQRALAQRRAATGLSRIAASPEKLS
                     SPELFDQASDLLDDAMALTPRGPRLDARIREVTALLAAYRDPIPVVFVSDNRTQVTLS
                     TIGRLGSFERKQLDLRPGSYTVIGSRDGCRDVRADIVVKPNMQPVDIRCTERL"
     gene            complement(4749..5945)
                     /locus_tag="AC16QF_11780"
     CDS             complement(4749..5945)
                     /locus_tag="AC16QF_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449282.1"
                     /translation="MKLLAVALLAGLCAGCVQEVVRTVDMTPPEQLDRVQTEDELLDI
                     GVAILDPNVPESYDEQIKQLVQPEIRRAEANFIPYVAKNLLQSTGNWGAVRVVPRPTH
                     AVDVVVEGKILHSTGENLAVEMSVHDATGRHWFSRRYEALASKYAYEEAIPQSIDPFQ
                     AIYKQLADDMLDYRKGLSYDEVMVIRRTAEMKFAQDFAPDAFADHITTTSEGEFELKR
                     LPAENDPMLSRVQRVREREYLFIDTLDEYYESFHRDMFESYQSWRRATYDEAIAYKAL
                     RAQSRARTIGGATAIIAGVAAMSESSDPYVDAGGIVSVIGGAMTIKGAIEKRAEAQIH
                     AEVLQEVGVAAEAEIVPYTIELENQTVRLQGSVDEQYEELRRILRRIYFEDLGLPAPE
                     EQPEET"
     gene            complement(5991..6689)
                     /locus_tag="AC16QF_11785"
     CDS             complement(5991..6689)
                     /locus_tag="AC16QF_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449283.1"
                     /translation="MPGSPEGPDSGEPGEAGPGEKEPGVAGTEEAGSGQEQPGASGAG
                     DPGEDSPGWVDGDEAGEGGWETSNQVPAQTAEMPPMPSGSGGGPAEQAEPGQSEGDGE
                     LDKALESFDGEIMSEREVIMARSNENAGKTGSSIPMPGSGSGSGSQTGGSEGVPGAEQ
                     GGSDVPGGPAGMPRASSAPPPPMASAGDIPDDIPDAKDDDIIARQLREAAMKETDPEL
                     KEKLWDEYRRYKGA"
     gene            6805..>6997
                     /locus_tag="AC16QF_11790"
     CDS             6805..>6997
                     /locus_tag="AC16QF_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449284.1"
                     /translation="MLLPLVGGAPPGWLGEDWGLCLPGFDGELGWLGELLGWLGELLG
                     EEGGVDGLGMLGGFGGWGGV"
BASE COUNT         1072 a         2289 c         2307 g         1329 t
ORIGIN      
        1 cgccgaagag gatctcgttt tcccggacca gagcgactcc cggcgcgtga taggccagcg
       61 tctcgcccga gtgcagcgac aggtccgagt cgttgagctc gaggacgtag tccatcagtc
      121 gcgcacctga agatgacgca gcagcctctc gtcgcagtaa ttctgggcgt ccagaaccag
      181 tcgctcctgg atgagctgga gctgatgcat gaggaacatg atcacgatgc tgatcaccag
      241 agcggtaaag gttgagttga acgcgacgcc caggctcgtg gtgacgcccg caatgttgcc
      301 ctcgacagcc tcgtaggcct gcccgagggc ctcgctgata ccccttaccg tgccgatgaa
      361 cccgatggag ggaattgccc acgcgatgta gcggaccatg gacagctcgc tgtcgagccg
      421 atcggactct gactcgcaaa ccgaccgcac ggcttcggag acgtcctgca cgttacgggt
      481 ggaggcaaac cgctgcaggg cagccatcag ggccctcggc agcaggtagt ttcgattctc
      541 gtccggcaga gcctggacgg cccgcgcgta ctcccgagtg tcctccggga gaacgctcat
      601 gccttcactg atcttgacga tgtcttcctg cagcaggctt cgctcgcgca acgcgctgcg
      661 ggtcttgtac cccatgatgg ccatggccca gagcatcagc acgaaacagg actcctgctc
      721 gtagtccttg agcaccacat aaagggagcg ctcctgggcg tatgcctcgc cggctgcagc
      781 acgttcggcc tgggcggcga tgatggcgtc ggcattcggt ctgatgaccg tgacgtagac
      841 cagatgaacg aggatcaccg caaggaccag cgctgacgac tggtaaatga gttcaaaggg
      901 tagctgacgc ttcatttgct gtggttccag acgcgattag atgtctacgg ggaatgagac
      961 cgcgaagtcc tccgaaatct cctcgctggg cacgaagatc tcggggctgg gctcgccgtc
     1021 cgcctcctct tcttccccgg cgcgatcgcg ggtgtcggca gcttcctcgg cagccacggc
     1081 gaacccggcg cccatggtcg acgcaagcag cgctgccgcc gcgaaaagga gagggccttt
     1141 cacgacggct ccgcgaaccg cgcgatgcga aaaccgacgt cttcgcgacc atcaacaccg
     1201 tagtcgcgaa acgacaggcg caggtcagtg atggttccgt tcatccagct ggatcctctg
     1261 atcacgcgat actccccggt gtccggtccc aggggatcct tcgtcggttc agcccccgga
     1321 atctcgtaga agtcgttcac ccactccgca acgttgcccg ccagatcata gatgccgatg
     1381 gcgttgggcg cgaacgtccc ggtctcggcg gagacgatgt ggttgtcgtt gtagccgaac
     1441 accacgcgcc ccaccaggtg tgcggccgac cggtcggcgt agttgccgtg acgatcgggg
     1501 ggcggcagcg cagttcccca cgggaaacga tatccggtgc tgtccgggcc gttctggcgg
     1561 gccgcccact cccactccgc ttcggtgggc aaccggtagc cggtagccgc ggggttcgtt
     1621 gcgaccactt tgccaaggtc cgtggcatag aacgggggta acccttccct ggccgacaac
     1681 cagttgcagt agagggcggc ctcctgccag gagacgccaa ccaccggctg atcatccttg
     1741 ttgagctcgt gatcctgaaa ttcccccgag tcatgcccgg gcgcgaaatc gagaaactgc
     1801 gcgttggtca cctcggtccg gctgagatag aaaatccgct ccatgcccac ctctcggagc
     1861 gtctcgttgg ctcgccggcc tggctcgcgc cgggaagcgc ccatggtgaa ggggcccgga
     1921 ttcagcagca cgagttcctg gccctggctg gtctggatga tcggtttcat ggctgccaga
     1981 cgagcctctt ccagggtcag aaggcggacc ttgatttcct gggtgacccc gttgcgcggc
     2041 gttatctccg tggagtaacc cgcgtatcca tccagcgcga tctcgacccg atgggtcacg
     2101 gtgggcaggg tcagcgtctg gttggcgttg cccagattac ggccgtcgac gaagagctcg
     2161 gcttcggcgg gctgggtcgt gacgatgagc tgacctgtaa ggcgcgtgag cgtcaggtct
     2221 accgtgcgct caccgccgga ggccacccga acgctgcgct cgccgggggc gaaacctgcc
     2281 ttgaagatct tcacccggta gctgataccg gatttcaccg cgatctccac aggcgtctcg
     2341 ccctgaaact gcccgttcag cgtgacgccc gctccagccg gtcggctcgt gacctgcagc
     2401 agcccgtcgg cgcggagcag ggtgacttcc ggcagcgtca cctcatccag cgcggcgacc
     2461 aggatgcgtt gccgatgccc gcggtggccg ggcgccttga gccttatctc gtgctcgccg
     2521 gtgggcacct cgatgacggc tggggtaaga cgacccgtgg gttggtcgtt caggaggatc
     2581 tccgctccgg ccgggatgct ggaaacgctc acctcggccc agtcgggaag cagggaaccc
     2641 tccacggtct gagctcgatc cagaccttcg atctcggcct ccatatccag acgctggtag
     2701 cggtccagct tcatggatat cgtcgccggg ccggcaggga ccttgacacc gtccagtggc
     2761 gttcgtccga gcagcgcccc ggccacgatg acctccgcgc cgtcgggatt gctcagaaag
     2821 gtgacccgac cgggaagctt cgtgaactcg aagcgatgtg tctggctgcg cgcgtcgccg
     2881 acgacgagga gctcgtcgag tggctcgtag ccctcggcgc gagcacttac ccgatactcg
     2941 ccgtcgagca tcagataaat gtcgcccagg gcaatggccg gaccttcgct cacgctctct
     3001 gaggcggccg gggggtcgaa ctccagccgc acggatttcg cggtgaagag aaaccacatg
     3061 atcgccatca cgggaatcgc tacgagcacg gcgccgatct gaaacggcgt cagcggcagc
     3121 tttcgtttcg gcgcctgctc ccgaggcgtg tagcttaccg cctcgatgat ctccggatcg
     3181 tcggcctgaa cctgggaggc gttgttgtcc tggccgtttc gagtgctcac gacaagccta
     3241 gagccgctcg gtgcagcgga tgtcaacggg ctgcatgttc ggtttgacga cgatgtcggc
     3301 cctgacatcg cggcagccgt cacggctgcc gatgaccgtg taggagcctg gccgcaggtc
     3361 gagttgcttg cgctcgaagc tgcccagcct gccgatggtg gacagggtga cctgggttcg
     3421 gttgtccgag acgaagacga cgggaatcgg gtcccgatac gcggcgagca gggcggtgac
     3481 ttccctgatc cgagcgtcga gccgtggtcc ccggggcgtc agggccatgg cgtcatcgag
     3541 caggtcgctc gcctgatcga atagttccgg cgacgagagc ttctccgggc tggccgctat
     3601 ccgcgagagg ccggttgccg cgcgacgctg cgcgagcgcg cgttgacgcc cgtcgcgggc
     3661 aaactgaatg ttgccgtctg actcgagcac ggcgacgtag ctctgcaacg ccgtctccca
     3721 gtcctcggcc gcctcggccg cttcggccct gccgcgcagc gtcgccagac gccggccggc
     3781 agaggcgtcg tcgacctgtt cgaggcctcc gagcgccacc gggttgccgg gatccagctt
     3841 cagggcctgg ccaaaggcag cgcgcgcggc atcgagcctg ccggcctcca gcgccgcgaa
     3901 accggaggag agatggccct tgaccgtctc cgcctggatg ccggcgcggg cagcccggat
     3961 gcccgcttcg aggcccgcgg tcttcgggtc cattgcgcgc actcgctcgt agacggcgag
     4021 ggcgtcccga tactccgcgg ccagctcgtg attcttggct tcccgcatcg ccgtgttcac
     4081 ctcgggtagc agcgcggctc gccgcgcgcc ttgctgagct tcctgatcgt cgggagagat
     4141 ggtcaacgcc cgggcgaacg cccctgcagc atttttctcg tcgcgtcgtt ccagggccgt
     4201 gcgaccgtcc cgcatcgcgc ttgcgaaacg cgccttgccg tcctcgatga gggcggccag
     4261 cgcagttgcc gccttcgcgt agctttcgat ggcggcctcg aacgactccc cgagaaactg
     4321 ctcgtcgccg gcggtggcga ggcgcttggc ggcatcgtat tcggcctgtc cccattgccc
     4381 gacctgcatg gtctgctcca ggtcaagctg gagctccacg aaccgtgcca gctcctcctg
     4441 agccctggcc cgcgcctgct ccttcatcag cgcctgaaat ggaggcagct tctcgtccct
     4501 ggccgccggg cgtggcgagt ccgaggtcgc gccggcggta tcggtggccg gcgtgccggg
     4561 gagcgtcggg ggttggacca gatcggggac gaccacgaag acgacgaagg ctgtcgccat
     4621 gacgagcagc acggaggcgc ccgctgccag gggcctgagc gaggggcgcg tggaggccga
     4681 cggccgttcc ggagcgctgt gcgtgagcgg gaccgattta ggccggatct cgccgaaatc
     4741 ttcgctcatc aggtctcttc tggttgttct tcgggagcag gtagacccag atcctcaaag
     4801 taaatgcggc ggagaatcct acgcagctct tcgtactgct cgtccaccga cccctggagc
     4861 ctcacggtct ggttctccag ctcgatggtg taggggacga tctcagcttc cgcggcgaca
     4921 ccgacttcct gcagcacctc cgcgtgaatc tgcgcctcgg cgcgtttctc gatggcacct
     4981 ttgatggtca tggcgccgcc aatgacgctc acgatgccgc cggcgtccac gtatgggtcg
     5041 ctgctttcgc tcatggcggc gaccccggca atgatggccg tggcgccgcc gatggtccga
     5101 gcgcgcgact gcgccctcag cgccttgtag gcgatggctt cgtcgtaggt cgctcggcgc
     5161 cagctctggt aggactcgaa catgtcgcgg tggaaactct cgtagtactc gtccagcgtg
     5221 tctatgaaga ggtactcgcg ctcccgaacg cgttggacgc ggctcagcat ggggtcgttc
     5281 tcagcgggaa gacgtttgag ctcgaactct ccctcggagg tggtggtgat gtggtcggcg
     5341 aacgcgtccg gcgcgaaatc ctgcgcgaac ttcatctccg cggtgcggcg gatcaccatg
     5401 acctcgtcgt agctcaggcc cttgcgatag tcgagcatgt cgtcggccag ctgcttgtag
     5461 atggcttgaa acgggtctat ggactgcgga atcgcctctt cgtaggcgta tttgctggcc
     5521 agcgcttcgt agcgccggga aaaccagtgg cggccggtcg catcgtgcac cgacatctcc
     5581 acggccaggt tctcgcctgt ggaatggaga atcttgccct caacgacgac gtcgacggcg
     5641 tgggtcgggc gcggcacgac gcgtacggcc ccccagtttc ccgtgctctg cagcaggttc
     5701 tttgcgacgt agggaatgaa gttggcctct gcgcggcgga tctccggctg aacgagctgc
     5761 ttgatctgct cgtcgtagct ctccggcacg ttcgggtcga gaatggccac gccgatgtcg
     5821 agcagctcgt cctcggtctg aacgcgatcg agctgctcgg gaggggtcat gtccacggtt
     5881 cggacgacct cctgaacgca gccggcgcag agaccggcga gcaacgccac tgcgagtaac
     5941 ttcaatcttt ctttcgagtc tggctgcaca cgcatttgcc tactgctggt tcaagcgccc
     6001 ttgtagcgcc gatactcgtc ccatagtttt tccttcagct ccgggtccgt ctccttcatc
     6061 gcggcctctc taagctggcg tgcaatgatg tcgtcgtcct tcgcgtcggg gatgtcgtcg
     6121 gggatgtcgc ctgccgaggc catgggaggc ggcggtgcag agctcgccct gggcataccc
     6181 gcggggccgc ccgggacgtc ggagcctccc tgctccgcgc cgggaacgcc ttccgacccg
     6241 ccggtctggc ttccgctgcc gctcccggaa cccggcatgg gaatcgacga gccggttttt
     6301 ccggcgtttt cgttggagcg tgccatgatg acctcccgct ccgacatgat ctcgccgtcg
     6361 aaactctcca gcgccttgtc gagctctccg tcaccctcgg attggcccgg ttcggcctgc
     6421 tctgcggggc cgcctccgga acctgacggc atgggcggca tctcggccgt ctgcgctggc
     6481 acctggttgc tggtctccca acccccttca cccgcctcgt cgccatccac ccaaccgggc
     6541 gaatcctctc cgggatcgcc ggcaccggat gcgccaggct gctcctgtcc gctacctgct
     6601 tcctcggtgc ccgcgacgcc cggctccttt tcgccgggtc cggcttcccc aggctcaccg
     6661 gaatccgggc cttctgggga tcccggcatt cccggcattc cgggcagctg ctcggtgccg
     6721 gtatcttcgc cggtgcccgc agtgctgtcg ggaggcggag gaatgtctgc ctcgccggct
     6781 ctgggctgac ctcccggggg cggggtgctg ctgccgctgg tcggcggagc gccgcctggc
     6841 tggctgggag aggactgggg gctctgtttg ccgggtttcg atggggaact cggctggctc
     6901 ggcgagctgc tgggctggct tggcgagctg ctcggcgaag agggtggtgt agacgggctc
     6961 ggcatgctgg gtggctttgg gggctggggc ggcgtct
//
LOCUS       DBNHLM010000252        14687 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_993805, whole genome shotgun sequence.
ACCESSION   DBNHLM010000252 DBNHLM010000000
VERSION     DBNHLM010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 14687)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14687)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14687
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_993805"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="AC16QF_11795"
     CDS             <1..664
                     /locus_tag="AC16QF_11795"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF004281.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HIR6449285.1"
                     /translation="AGTVLIVGMGGLGAPAAIYLASAGVGHLIISDHDRVEASNLQRQ
                     IVHHDASIGQTKVASACQSLLQTNPELRITGIDRKLEGDELLKIVGDADVVLDATDNF
                     AARFAINAACWEAGVPLVSGAAIRWEGQLAVFDPRDAASPCYRCLYAEGHDEALNCAE
                     NGVIAPLVGVIGTCQAIEAIKLLTGIGEPLTGYVLYFDAKRMDWRKLKLPKDPACPGC
                     SA"
     gene            complement(630..1277)
                     /gene="lipB"
                     /locus_tag="AC16QF_11800"
     CDS             complement(630..1277)
                     /gene="lipB"
                     /locus_tag="AC16QF_11800"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR6449286.1"
                     /translation="MADVPVIVRQLGRTRYEPVFRAMQVFTEERAEPTADELWFTEHD
                     PVFTQGQAGKAEHLLAPGDIPVVQTDRGGQVTYHGPGQIVAYLLFDLRRMGLSVRGLV
                     SGIEEAVVRLLADYDIRSAPRRDAPGVYVAGSKIAALGLRVRRGYSYHGLALNVQMDL
                     EPFSRINPCGLFGMEVTQLADLGGPADVQTVEDELLTKLADVYGFTPSSPDTQGP"
     gene            complement(1279..1542)
                     /locus_tag="AC16QF_11805"
     CDS             complement(1279..1542)
                     /locus_tag="AC16QF_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HP0495 family protein"
                     /protein_id="HIR6449287.1"
                     /translation="MTEPPKIEFPCDYPIRVIAENHLALRETVVRIVRLHAPDLAEST
                     VSVRNSREGRYCSVRFSITARGEPQLRALHRALMDEPMVKMVL"
     gene            complement(1535..2722)
                     /locus_tag="AC16QF_11810"
     CDS             complement(1535..2722)
                     /locus_tag="AC16QF_11810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR6449288.1"
                     /translation="MLRGLLMSFWANHLAAFCLGLAAVAGPAHAGEGPIIPPPPKIAG
                     TSYLLVDADTMKVLVENEIHEPVPPASLTKIMTGYIAAVELEAGRISLEDQVPISVKA
                     WRAPGSRMFVREGTRVGLEDLLRGIIVQSGNDASIAMAEHIAGSEDAFADMMDQQAIQ
                     LGMTTTQYQNATGLPSDDHYTSAWDLAVLTQSLIKRFPDSYKIYSERSFAFNDIEQPN
                     RNRLLWRDRTVDGVKTGHTSAAGYCLVASAKRGNMRLISVVLGTESEEARMRESQKLL
                     SYGFRYFETQKLYDAGVPLKTSKIWYGEADDIQLGLAEEVFVTIPRGHYEDLKAELSV
                     VKTIEAPVAAGQELGELRLHFGDELMLSAPLVALQEVKEADMFSRLLDGTRLLFRDLF
                     GVD"
     gene            complement(2750..3505)
                     /locus_tag="AC16QF_11815"
     CDS             complement(2750..3505)
                     /locus_tag="AC16QF_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HIR6449289.1"
                     /translation="MVVRLRAAGWLLVLTLVACSGPGVRPPERSDGPPAHTPVDLAKL
                     PDPIPREEPKSARGNPPSYTVMGRTYRVMDSAAGYESTGIASWYGRKFHGRATSSGET
                     FDMFELTAAHTSLPIPTYARVTNLHNGRSTIVRINDRGPFHADRLIDLSYAAAVKLGF
                     ATRGTARVRVEALVDSPDYFLQAGAFSDMSSADALKNALGALTGARAYVVRVSSDALF
                     RVRIGPVKSRPEALRLQALIASSDFDRPLILEQ"
     gene            complement(3499..4509)
                     /gene="mltB"
                     /locus_tag="AC16QF_11820"
     CDS             complement(3499..4509)
                     /gene="mltB"
                     /locus_tag="AC16QF_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HIR6449290.1"
                     /translation="MRYAFLWATTLLLLFGSAHADFAGRAEVREYIAEMVREHGFGEE
                     ELADVFADADRKESILEAIARPAERVLKWHEYRRIFLGEKRISQGIEFWREHAETLAR
                     AEAAYGVPPEYVVAIIGVETRYGRNRGGYRVVDALTTLAFDYPPRSAFFRDELTHFLL
                     LAREEGMDPRDPKGSYAGAMGYGQFIPSSYRSYAVDFDGDGLRDIWSNPTDAIGSVAN
                     YFKRHGWQSAQPVVFPVAAEGDGLSAVANKTLDLKLTVGELKDMGMRPAGEAAGLAED
                     TAAAIFRMEMEDGAEYWLGLKNFYVITRYNRSRLYALAVHQLSQELLERQRSTSAVTQ
                     AW"
     gene            complement(4566..5651)
                     /gene="rodA"
                     /locus_tag="AC16QF_11825"
     CDS             complement(4566..5651)
                     /gene="rodA"
                     /locus_tag="AC16QF_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR6449291.1"
                     /translation="MGAGRRLVQIHLDPLLLLLLLAIVGYGLVVLYSALNGNEELFSN
                     QAVRIGLALSVMLVAAQLEPRFYLRWSPVVYGVGLVLLVLVLLIGVKVKGSQRWLDLP
                     GLPRFQPSELMKIAVPMMVAWYFHERRLPPSFRDVCVAIVIIGLPAGCIILQPDLGTG
                     ILVACAGFAVVVLAGVYWRWIAFAAFGIAALAPGLWFLLQDYQRQRILTLFDPESDPL
                     GAGWNIIQSTTAIGSGGVWGKGLMQGTQSRLDFLPESQTDFIIAVIGEELGLVGIASL
                     LVLYLVVVARGLYIATRAVNTYQRLLAGALTLTFFVYVFVNIAMVSGLLPVVGVPLPL
                     VSYGGTSALTLMASFGIIMAIRTERAW"
     gene            complement(5693..7579)
                     /gene="mrdA"
                     /locus_tag="AC16QF_11830"
     CDS             complement(5693..7579)
                     /gene="mrdA"
                     /locus_tag="AC16QF_11830"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR6449292.1"
                     /translation="MPPVLALKDSSSERRSFSARAAVLFALATCLVSVLILRLLQLQV
                     WEYDTYRTRSEENRIQVQPLAPPRGLIFDRNGVLLADNRPVHSLALVRERIADLDALI
                     TELGSLVSISEAEIESLRGRLLRRRRPYEPVALKLTLSEEDIAVLAVNRHRLVGVEIS
                     TQLVRYYPFGPLLAHAVGSVRRVTEDDLRELDPVRYSATKFVGRRGVERFYERSLHGE
                     VGYQRVETDAHGRIRQVLEINEPAAGQNITLQLDSRLQIAASAALGDRRGAIVALDPR
                     SGGVLALVSHPQYDPNQFVTGMTSAQFRELTSSRDTPLFNRAINGQYAPGSTFKPIVG
                     LAGVASGVTRWADQIDDQGWYQLPGQERIYRDWSWAVDDSGGQGLTNLTRAIYRSSNV
                     YFYDLATRLDIDRIATFAAQFGYGQRTAVDIPDASKGLLPTRVWKKGAKGEIWYPGDN
                     VNIGIGQGDLLATPLQLATVAAVVANRGEFVRPQMLLSSDRPLLEFDPARPMPRVEGP
                     SAEDWERMVDAMEDVVHRGNRGVGQNGTAWAYIGRNIPYRMAGKSGTAQVVEIKQGEE
                     YDEEELEEYQRKHAWFIAFAPADDPKIAVSVLVENGGGGSSVAGPVAREVIDAFMLPQ
                     LAAR"
     gene            complement(7581..8048)
                     /gene="rlmH"
                     /locus_tag="AC16QF_11835"
     CDS             complement(7581..8048)
                     /gene="rlmH"
                     /locus_tag="AC16QF_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR6449293.1"
                     /translation="MKIRVIAVGARPRPWVADGFREYARRLPREMPLSLVEVAPGGGR
                     GRSAEQVVAMESERLLSHLGPRDWVVALDVGGEGCSTRELALSLESWRMQGRDVAFLI
                     GGAEGLAPTCLARADMALSLSELTFPHEMVRVILAEQIYRAWTILSGHPYHRD"
     gene            complement(8068..8415)
                     /gene="rsfS"
                     /locus_tag="AC16QF_11840"
     CDS             complement(8068..8415)
                     /gene="rsfS"
                     /locus_tag="AC16QF_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR6449294.1"
                     /translation="MTAEALRDLVVDALEERKGRDVVAMDVTKSTDITDFMIIASGTS
                     NRHVKALVDQVVESAKAGGNPPLGVEGRETHEWVLVDLGDVLVHVMQADTRAFYDLER
                     LWSDIPLDSETLN"
     gene            complement(8405..9037)
                     /gene="nadD"
                     /locus_tag="AC16QF_11845"
     CDS             complement(8405..9037)
                     /gene="nadD"
                     /locus_tag="AC16QF_11845"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR6449295.1"
                     /translation="MIALFGGTFDPVHRGHLHGAATVCEALGLEAISMVLSARPGHRA
                     SPGASAEHRWEMLCLACADDGRLVPDDRELHRDRPSYTVETLLEVRGEIGDESLVWVL
                     GSDAYADLTSWHRWGEVLDLTNLVVLERPGGPSRWAPPLDGISRRHRVEGMLDAPWGQ
                     ILFLKAPMQEVSASEIRAAIAGGRPVAHLLPERVATYISRHGLYGAPSDS"
     gene            complement(9034..10296)
                     /locus_tag="AC16QF_11850"
     CDS             complement(9034..10296)
                     /locus_tag="AC16QF_11850"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR6449296.1"
                     /translation="MTDIAAYMGEVARSARAASRVIAAASSDSKSRALKAMVEALDED
                     RETLKRANEVDVRAAGDKGVDQPLIDRLVLDDRAIDRMLEGVLQVDKLADPVGEITDL
                     AYRPTGIQVGRMRVPLGVIGIIYESRPNVTADAASLCLKSGNACILRGGSEAIESNRA
                     IAAGIARGLAAAGLPETVVQLVDTTDRDAVGELLKADRFVDVIVPRGGKGLIERIIRE
                     SRIPVIKHLDGVCHVYIDDAADLEMAAAIAVNSKIEKYAVCNAMETLLVANGVADRVL
                     PVLGDRFRQAGVELRGCERARAAFSDMKPATEEDWREEYLGPVLAVRVVEGIDEAIAH
                     INTYGSQHTDAIVSNDYGRTRRFLAEVDSASVMVNASTQFADGFEFGLGAEIGISTDK
                     LHARGPVGLEGLTSQKFVVFGGGEIRHR"
     gene            complement(10364..11152)
                     /locus_tag="AC16QF_11855"
     CDS             complement(10364..11152)
                     /locus_tag="AC16QF_11855"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR6449297.1"
                     /translation="MDWLQIIILALVQGITEFLPISSSAHLILPALLTEWPDQGLAFD
                     IAVHAGTLLAVLSYFRREIGRFAASGARLLARGQRDDDADLLLKLIAATLPIVLVGYL
                     AHDWVESSLRTVPVIASATIVFALALWWADGRRSGADHPSWTAAMLIGAAQVIAIVPG
                     TSRSGITMTAALLLGLSRTGAARFSFLLSIPTILGAAVLEAASLGAGEHAVRWAELVA
                     GAVLAGAGAYCCIHFFVKLVERTGMLPYVVYRLMLGTLLLGLAL"
     gene            11232..11825
                     /locus_tag="AC16QF_11860"
     CDS             11232..11825
                     /locus_tag="AC16QF_11860"
                     /inference="COORDINATES: protein motif:HMM:NF014269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR6449298.1"
                     /translation="MEIPLFPLRVVLFDGGRLPLQIFETRYLDMVSRCTRESIPFGVV
                     LIRGAGSDARLDPLANQPAIFEVGTEAHIVDFNQLPNGRLGIIVRGGRKFRVVSTREQ
                     ADHLLLGTVDFLPAEPDSTVPSEHAPLVDILKELVKHPGVQKLGLEIDFADARSVGAR
                     LAELLPIEPEIKQSLLQLQLPRERLQELTRLVNKFRD"
     gene            complement(11822..12265)
                     /gene="ssb"
                     /locus_tag="AC16QF_11865"
     CDS             complement(11822..12265)
                     /gene="ssb"
                     /locus_tag="AC16QF_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR6449299.1"
                     /translation="MARGINKVILIGNLGRDPETRYSQGGNAVTSFSVATTENWRDRQ
                     SGEQQERTEWHNVVCFARLAEIAGEYLRKGSKVYIEGSLRTSSWEQDGQKRYRTEVMA
                     RDLQMLDSRGGGQGGQGGGFESAAPAASENAPTLADDDFDDDIPF"
     gene            12475..>14687
                     /gene="uvrA"
                     /locus_tag="AC16QF_11870"
     CDS             12475..>14687
                     /gene="uvrA"
                     /locus_tag="AC16QF_11870"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR6449300.1"
                     /translation="MDSIAVRGARTHNLKNVNLDIPRDRLVVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSMMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIYDYLRLLYARVGEPRCPNHDLPLDAQTVSQMVDQVLGLPEDKRVMVLAPVVTERKG
                     EHLHVFQELMANGFIRARIDGIVVDLDHPPNLEKNKKHTVEAVIDRLRIRADAQQRLA
                     ESFETALDLAEGIAKVASMDDADEPELVFSARFACPACGYSISELEPRMFSFNNPAGA
                     CQACDGLGVKQFFDPGLVVQDEELTLAEGAIRGWDRRNVYYFHMLSSLAAHYGFDVET
                     PFRKLPKKHRDAILYGSGRERIDFSYANDRGDVIHRRHRFEGVIPNMERRYHETDSSL
                     VRENLQKYLRVRSCPDCGGTRLREASRNVFIDGVSLPEITSGSVEGSLAYFDELKLSG
                     RRGEIADKILKEIRARLRFLVDVGLNYLTLDRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYILDEPSIGLHQRDNARLLKTLEHLRDLGNTVLVVEHDEEAIRSADHIIDIGPGAGV
                     HGGCVVAAGSLEKILKSRRSITGQYLSGRKKIPVPEKRTAFVEEETIRIIGATGNNLQ
                     SVSVEIPCGLLTCVTGVSGSGKSTLINHTLYPVAATRLNKATTLTPAPVEEVLGLNHV
                     DKVVDIDQSPIGRTPRSNPATYTGLFTPIRELFAQTQEARTRGYKPGRFSFNVKGG"
BASE COUNT         2754 a         4917 c         4553 g         2463 t
ORIGIN      
        1 cgccgggacc gtgctcatcg tcggcatggg tgggctcggc gcgcccgccg ccatttacct
       61 ggcaagcgca ggcgtgggcc acctcatcat cagcgatcac gatcgggtgg aggcttccaa
      121 cctgcagcga cagatcgtcc accacgacgc cagcatcggc cagaccaagg tggcatcggc
      181 atgccagtcg ctgctgcaga ccaacccgga actgcggatc accggcatcg accgaaagct
      241 ggagggggac gagctgctaa agatcgtggg tgacgccgac gtggtgctgg acgccaccga
      301 caactttgcc gcccgcttcg cgatcaacgc cgcctgctgg gaggccggag tccccctggt
      361 ctcaggcgcc gccatccgct gggaaggtca gctcgccgtc ttcgacccgc gggatgcagc
      421 gagcccctgt taccgctgcc tttacgccga gggacacgac gaggcgctga actgcgccga
      481 gaacggggtc atcgcccctc tggtgggagt catcggcacc tgccaggcca tagaagccat
      541 caagctgctc accggaatcg gcgagccgtt gaccggctac gtcctctact tcgacgccaa
      601 acgcatggat tggcgaaagc tgaagctgcc taaggaccct gcgtgtccgg gctgctcggc
      661 gtgaacccgt agacgtcggc cagcttcgtc aggagctcat cctcgacggt ctgcacgtcc
      721 gccggaccgc ccaggtcggc aagctgcgtc acctccatgc cgaacaaccc gcacgggttg
      781 atgcgcgaga acggctctaa gtccatctgc acattgagag ccagtccgtg gtaggagtac
      841 ccgcgccgaa ctctcagccc gagagccgcg atcttagacc cggccacgta gacccccggc
      901 gcatcacggc gcggcgcgct gcggatatcg tagtcggcca gcagcctgac gacggcttcc
      961 tcgatgccgc tgaccaggcc gcgcacgctc agccccatgc gacgcaggtc gaacagcaga
     1021 taggcgacga tctggcccgg gccgtgatag gtcacctgcc cgccccggtc agtttgcacc
     1081 accggaatgt cgcccggcgc caggaggtgc tcggccttgc ccgcctgacc ctgggtgaac
     1141 accggatcgt gctcggtgaa ccagagctca tcggcggtgg gctccgcccg ttcctcggtg
     1201 aagacctgca tggctcgaaa taccggctcg taacgggtcc gccccaactg cctgacgatt
     1261 acggggacat cagccacctc acagcaccat cttgaccatg ggttcgtcca tgagagcccg
     1321 atgcagggcc cgcagctggg gttcccccct ggcggttatc gagaagcgca ccgagcagta
     1381 gcggccttcg cggctgttcc tgaccgatac ggtgctctcc gccaggtccg gggcatgcag
     1441 gcgaacgatc cgcaccacgg tctcgcggag cgcgaggtga ttctccgcga tcacccggat
     1501 ggggtagtcg cagggaaact cgattttcgg gggttcagtc aacgccgaaa aggtcgcgaa
     1561 agaggagccg ggtgccgtcc agcaggcgcg agaacatgtc tgcttccttg acttcctgca
     1621 acgcgaccag gggcgcgctc aacatcagct cgtcaccgaa gtgcaaacgc agttccccca
     1681 gctcctgacc ggcagctacg ggagcctcga tggtcttcac cacgctgagc tcggccttga
     1741 ggtcttcgta gtggccccgg gggatggtta cgaaaacctc ctctgcaagg cccagctgga
     1801 tgtcgtccgc ctcgccgtac cagatcttcg aggtcttcaa agggacaccg gcatcgtaga
     1861 gtttctgcgt ctcgaaatag cgaaatccgt aggaaagcag cttctggctc tcgcgcatcc
     1921 tggcctcttc gctttccgtg cccagcacga cggaaatcaa tcgcatgttg ccgcgcttgg
     1981 ccgaggcgac caggcagtag ccggcggctg aggtgtgccc ggtcttgacc ccgtccacgg
     2041 tgcggtcccg ccagagcagc cggttgcgat tgggctgctc gatgtcgttg aaggcgaaag
     2101 atcgctcgga gtagatcttg tagctgtcgg gaaaccgctt gatgagcgac tgggtgagca
     2161 ccgccagatc ccaggcggag gtgtaatgat cgtcgctggg aaggcccgtg gcgttctgat
     2221 actgggtcgt ggtcatgccg agctggatcg cctgctgatc catcatgtca gcgaaagcat
     2281 cttcactccc ggcaatgtgc tcggccatgg cgatgctggc atcgttgccc gactgtacga
     2341 tgatgccacg aagcaggtct tccaacccga cccgggtgcc ctcccgcacg aacatccgcg
     2401 acccgggcgc acgccacgct ttgacgctga tgggcacctg atcttccagg ctgatccggc
     2461 ctgcctccag ctctacggcg gcgatgtagc cggtcatgat cttggtcagg ctggccggtg
     2521 gcacgggctc atgtatttcg ttttccacca gcaccttcat ggtgtccgca tctaccagga
     2581 ggtaagaggt gccggcgatc ttcggcggcg gcggaatgat gggcccctcc cccgcgtggg
     2641 cgggaccggc aacagccgcg aggcccagac agaaggctgc gagatgattg gcccagaacg
     2701 acatgagaag acctctcagc atgctcgtac gcttccggtg gattctagct cattgctcga
     2761 gtatgagcgg gcgatcgaag tcagaagacg cgatcagcgc ctgcaaacga agcgcttccg
     2821 gcctggactt gaccggcccg atacggaccc ggaacagagc atcgctggaa accctcacca
     2881 cataggcccg cgctccggtc agcgcgccga gggcattctt gagcgcatca gccgagctca
     2941 tgtcgctgaa agcaccagcc tgcagaaagt agtcggggct atccaccaaa gcctccacgc
     3001 gcacccgcgc cgtgccccgg gtcgcgaagc ccagcttcac ggcggccgcg taggaaaggt
     3061 cgatgagccg atcggcgtga aacggaccgc gatcattgat ccgaacgata gtggaacgcc
     3121 cgttgtgcag attggtcacc cgggcgtagg tgggaatcgg cagcgaagtg tgagcggccg
     3181 tcagctcgaa catgtcgaac gtctcgccgc tcgacgtcgc tcgtccatgg aacttgcggc
     3241 cgtaccagct ggcgattccc gtggactcgt agcccgccgc gctgtccatc acccggtagg
     3301 tgcgccccat caccgtgtag ctcggcgggt tgccgcgggc gcttttgggc tcctcgcggg
     3361 gtatcgggtc aggcagcttg gcgaggtcaa ccggcgtgtg cgcgggcgga ccatcgctac
     3421 gttctggcgg gcgcacgcca gggccgctgc aggcaaccag ggtgagcacc agcagccagc
     3481 ccgccgcgcg caagcggact accaagcctg ggtcaccgcg ctggtgctgc gctgccgctc
     3541 caagagctcc tgactgagct ggtgaacggc cagcgcgtac agcctgctgc ggttgtagcg
     3601 cgtgatcacg tagaaatttt tgagccctag ccaatattcc gctccatcct ccatttccat
     3661 acgaaagatc gctgctgcgg tatcttcggc gagaccggcg gcctcgccgg ccggcctcat
     3721 gcccatatcc ttaagctcac cgaccgtcag cttgaggtca agggtcttgt tggccacggc
     3781 agagagcccg tcgccctccg cggcgacggg aaataccacc ggttgcgcgc tttgccagcc
     3841 atgccgcttg aagtagttgg ccacgctgcc gatggcgtcc gtggggttgc tccagatatc
     3901 ccgcagaccg tctccgtcaa agtccacggc atagctgcga tagctggaag gaatgaactg
     3961 accgtatccc atggcgccgg cataggagcc tttcggatcc cgcggatcca tgccctcctc
     4021 ccgggcgagc agcagaaaat gggtgagctc gtcacgaaag aaagcggagc gcggcgggta
     4081 gtcaaaggcg agcgtcgtca gagcgtccac gacccggtat ccaccccgat tgcgcccgta
     4141 gcgagtctcc accccgatga tcgccacgac gtactcgggc ggcacgccat aggccgcttc
     4201 tgctcgtgcc agggtttcgg cgtgctcgcg ccagaactcg ataccctgag agatgcgctt
     4261 ctctccgagg aatatccgcc gatactcgtg ccacttcagc acccgctcgg cgggacgggc
     4321 aatggcctcg aggatgcttt ctttgcgatc ggcatcggca aagacgtcgg caagttcctc
     4381 ctccccgaaa ccgtgctccc ggaccatctc ggcaatgtac tcgcgtacct cagcccgacc
     4441 ggcaaagtcc gcgtgcgcgg agccgaacag gagaaggagg gtggttgccc agagaaaagc
     4501 gtatcgcatg aatctaccta tccgaacgtg tccaatgtga aagaagggac ccgaaaccag
     4561 gcgcactacc atgccctctc ggtgcggatg gccatgatga tgccaaaact cgccatgagc
     4621 gtgagcgcag acgtgcctcc gtagctgacc agcggcagcg gcacgccgac gaccggcagc
     4681 agcccgctca ccatagcgat gttgacgaac acgtaaacga agaacgtcag cgtcagcgcg
     4741 cctgccagca ggcgctgata cgtattcacc gcgcgcgtcg cgatgtacag cccccgggcg
     4801 accacgacga gataaagaac gagcagagag gcaatgccaa ccagcccgag ctcctcgccg
     4861 atgacggcga tgatgaaatc cgtctggctc tcgggcagga aatccaggcg gctctgcgtg
     4921 ccttgcatca gccccttgcc ccacacgccg ccagacccga tggccgtcgt cgactgaatg
     4981 atgttccagc cggcgcccag gggatcgctt tcgggatcaa acagggtgag gatccgctgt
     5041 cgctgatagt cctgaagcag gaaccaaagg cccggggcaa gggcggcgat gccgaacgcg
     5101 gcgaaggcta tccatcgcca gtagacgccg gccagcacca ccacggcaaa gcccgcgcag
     5161 gccacgagga tgcccgttcc cagatccggc tgcaggatga tgcaaccggc cggcaggccg
     5221 atgatgacga tggcgacaca gacgtcgcgg aagctcggcg gcaggcgtcg ctcatggaag
     5281 taccaggcca ccatcatggg cacggcgatc ttcatgagct ccgaaggctg gaatctgggc
     5341 agtccaggca ggtcgagcca ccgctgggag cctttgacct tcacaccgat taggaggacg
     5401 agcacgagaa gcaccaggcc cacgccgtag acgacgggcg accagcgcag atagaagcgc
     5461 ggctccagct gcgctgccac cagcatcacc gagagcgcca ggccgatgcg cacagcctga
     5521 ttcgaaaaca gctcctcgtt gccgttcagg gcgctgtaga ggaccaccag tccgtagccc
     5581 acgatggcca gcagaagcag aagcagcagc gggtcgaggt gaatctgcac cagacggcga
     5641 cctgcgccca tggcccggcc gccggggagg tgcctgacga agtcctgatt gctcaccgcg
     5701 cggccagctg aggaagcatg aaagcatcga tgacctcacg ggcaacgggt cccgccaccg
     5761 agctgccacc cccgccattc tccaccagga cggacaccgc gatcttcggg tcgtcggccg
     5821 gcgcaaacgc aatgaaccag gcgtgtttgc gctggtactc ctcgagttcc tcctcgtcgt
     5881 actcttcgcc ctgcttgatc tccaccacct gggcggtgcc ggacttgccg gccattcgat
     5941 acggaatgtt ccgaccgatg taagcccagg cggtcccgtt ctgacccacc ccgcgattgc
     6001 ctcggtgcac cacgtcttcc atggcatcga ccatgcgctc ccaatcctcg gcgctcggcc
     6061 cttctacccg gggcatcggt ctggccggat cgaactccag gagcggcctg tcgctggaaa
     6121 gcagcatctg agggcgaaca aactctccgc ggttggccac cacggccgcc acggtggcga
     6181 gctggagcgg ggtggcgagc aggtcgccct gaccgattcc gatgttgacg ttgtcacccg
     6241 gataccagat ctcgcccttc gcgcctttct tccatacccg cgttggcagc aggcccttgc
     6301 tcgcatcggg aatgtcgacc gcggtgcgct gcccgtagcc gaactgggcg gcgaaggtgg
     6361 cgatccggtc gatgtccagc cgggtggcaa gatcgtagaa gtagacgttg gaagaccggt
     6421 aaatggctcg cgtcaggttg gtgaggccct gacctcccga gtcgtctacc gcccagctcc
     6481 agtcccggta tatgcgctcc tgcccgggca gctggtacca cccctgatcg tcgatctgat
     6541 ccgcccacct cgtcacgccg ctcgcgaccc ccgcgagccc cacaatgggt ttgaaggtcg
     6601 agcccggcgc gtactggccg ttgatcgcac gattgaacag cggggtgtcc cgagaggacg
     6661 tcagctcccg aaactgggcc gacgtcatgc cggtcacgaa ctggttgggg tcgtactgcg
     6721 ggtgactcac cagggccagc acgccaccgc tgcgggggtc caaggccacg atggcgccgc
     6781 gccgatctcc cagggcagcc gacgcggcaa tctgcaggcg gctgtccagc tgaagcgtga
     6841 tgttctgacc ggccgcaggc tcattgatct ccagcacctg gcggatccgg ccgtgggcat
     6901 cggtctccac cctctgatag cccacctcgc cgtgcagcga gcgttcgtaa aagcgctcca
     6961 cccctctgcg cccgacaaat ttagtggcgc tgtagcgaac ggggtctagc tcgcgcagat
     7021 cgtcctcggt gactcgtcga accgagccca cagcgtgcgc aagaagcgga ccaaaggggt
     7081 agtagcggac gagttgggtg gagatctcaa cgccgacgag ccggtggcgg tttacggcaa
     7141 gcacggcgat gtcttcctcg ctgagcgtca gcttgagcgc caccggctcg tagggccgtc
     7201 ggcgtcgcag caggcggccg cggaggcttt cgatctccgc ctcgctgatg ctgaccagcg
     7261 agcccagctc ggtgatgagg gcatcgaggt cggctattcg ttcgcgcacc agagcaaggc
     7321 tgtgaacggg acgattgtcg gccagcagca cgccgttgcg atcaaaaatt agccctcgtg
     7381 gaggcgccag cggctgaacc tgtatgcgat tctcttccga ccgggtccgg tacgtgtcgt
     7441 actcccacac ctgcagctgc agcagacgga gtatcaggac ggatacgagg caggtagcga
     7501 gcgcgaacag caccgcggcc cgggcagaga aggaccggcg ttcggacgag ctgtctttga
     7561 gtgcgagaac aggcggcatg tcagtcccga tgataggggt ggccggagag aatggtccag
     7621 gcccggtaga tctgctccgc caggattacc ctcaccatct cgtgggggaa agtcagctcg
     7681 gacagcgaca gcgccatgtc agcacgagcc aggcacgtgg gcgcgagccc ttcggcgccc
     7741 ccaatgagga aagcgacgtc ccgcccctgc atccgccagc tctccaggct cagcgccagt
     7801 tcccgggtgc tgcaaccctc acctcctaca tccagggcga cgacccagtc tcttggaccc
     7861 aggtggctga gtaatcgttc cgattccatc gccacgacct gctcggcaga ccggccacgg
     7921 cccccgccgg gcgccacctc cacaagagac agcggcattt cccgcggcaa ccgcctggcg
     7981 tactcgcgaa acccgtcggc aacccagggc cgaggtcgtg cgcccacggc gatgacccgg
     8041 atcttcatgg cagccttccc gggcgcctca gttgagcgtt tcggagtcca acggtatgtc
     8101 ggaccagagc cgctcgaggt cgtagaatgc ccgagtgtcg gcctgcatca cgtgcacgag
     8161 cacgtcaccg agatccacca gcacccactc gtgggtttca cggccctcca cgccgagcgg
     8221 cgggttaccg ccggctttcg ccgactccac cacctgatcg acaagtgcct tcacatgccg
     8281 attggaggtg ccgctggcga tgatcatgaa atccgtgatg tcggtactct tggtcacgtc
     8341 catcgccacg acgtcgcggc ctttccgttc ttccaacgca tccacgacca gatcgcgcag
     8401 ggcctcagct gtcactcggt gctccgtata gtccgtgccg actaatatat gttgccactc
     8461 gctccgggag caaatgcgcc acggggcgcc cgccggcgat ggctgcgcga atttccgagg
     8521 cggacacctc ctgcatggga gctttcagga agagaatctg cccccacggc gcgtctagca
     8581 tcccctcgac ccgatgacga cggctgattc catccagggg cggggcccag cggctcggcc
     8641 cgcccggccg ctccagcacc acgaggttgg tcagatccag aacttcaccc cagcgatgcc
     8701 agctggtcag atctgcgtag gcgtcgctgc cgagcaccca gaccagggac tcgtcaccga
     8761 tttctccgcg gacctccagc agcgtctcca ccgtgtagga cggccggtca cgatgaagct
     8821 cgcgatcatc cggaaccagg cgcccatcgt cggcgcacgc caggcacaac atctcccagc
     8881 ggtgctcggc ggacgctccc ggcgaagcgc ggtgacccgg ccgggcggac agcaccatgg
     8941 agatggcttc gaggccgagc gcctcgcaga ccgtggccgc gccgtgcaga tgtccccggt
     9001 gaaccggatc gaaggtgccg ccaaacagag caatcaacgg tgccggatct ctccgccgcc
     9061 gaagaccacg aacttctgcg acgtgaggcc ttcgaggccc accgggccac gggcatggag
     9121 cttgtccgtc gagatgccga tctcggctcc caggccgaat tcgaagccat ctgcaaactg
     9181 ggtcgaggcg ttgaccatga ccgacgccga gtccacctcc gcaagaaatc gacgggttct
     9241 gccatagtcg ttggagacga tggcgtccgt atgctgggac ccgtaggtgt tgatgtgagc
     9301 gatcgcctcg tcgatcccct ccaccaccct caccgccagc accggcccga gatactcctc
     9361 ccgccagtct tcctcggttg ccggtttcat gtccgaaaag gcggcccgag cccgctcgca
     9421 gcctcgcaat tccacgcccg cctggcgaaa acggtcgccg agcacgggca ggacccgatc
     9481 ggctacgccg ttggccacca gcagcgtctc catggcgttg cagaccgcgt acttctcgat
     9541 cttggagttg acggcgatgg cggccgccat ctccaggtca gcggcgtcgt cgatgtagac
     9601 gtggcacacg ccgtcgaggt gcttgatgac cgggattcgt gattcccgga tgatgcgctc
     9661 gatcaggccc ttgcctcccc gaggcacgat gacgtccacg aaccgatccg ccttgagcag
     9721 ctctcccacc gcatcgcgat ccgtcgtgtc gacgagctgg acgaccgtct ccggcagtcc
     9781 tgccgcggcc aggccacggg caattcccgc ggcgatggct cggttcgact cgatggcttc
     9841 cgaaccgccc cgaaggatgc aggcgttgcc cgacttcaaa cacaggctgg cagcgtccgc
     9901 ggtcacgttc gggcgcgatt cgtagatgat cccgatcaca cccaggggca cgcgcatccg
     9961 ccccacctga atgcccgtcg gacggtaggc cagatcggtg atctccccca ccggatcggc
    10021 cagcttgtcc acctggagga cgccttccag cattcggtcg atggcacgat cgtcgagaac
    10081 cagacgatcg atgaggggct gatcgacgcc cttgtctccc gcggcgcgca cgtcgacctc
    10141 gttagcccgc ttcagcgttt cccgatcttc atcgagagcc tccaccatgg ctttgagcgc
    10201 ccttgacttg ctgtcgctgg aggccgcagc gatcactctg gaggcagcgc gagcgctcct
    10261 ggcgacctcg cccatgtaag cggcaatgtc tgtcatgact ttcgggggca tttggcccag
    10321 agattaggga ccggcaagta taccggaggt cagcgcgcga cgatcacagg gcgagaccga
    10381 gcagcaaggt gcccagcatc agccgataga cgacgtaggg cagcatcccc gtccgctcca
    10441 ccagcttcac gaaaaagtgt atgcagcagt aggcgcctgc cccggcaagc accgcgccgg
    10501 cgaccagctc cgcccatcga accgcatgct cgccggcacc cagagacgcc gcttccagca
    10561 cggccgcgcc cagaatcgtg ggtatggaga gcagaaagga gaaccgggcc gcacccgtac
    10621 gactcaagcc cagcagcagc gcggccgtca tggtaatacc cgaccgggag gtgccgggca
    10681 cgatggcgat gacctgcgca gcgccgatga gcattgccgc cgtccacgac ggatgatcgg
    10741 cgccgctccg gcgcccgtcc gcccaccaca gagcaagcgc aaagacgatg gtggcagaag
    10801 cgatgacggg cacagtgcgc aggctggact ccacccagtc gtgcgcgaga tagcccacca
    10861 gaacgatggg caacgtcgcg gcaatcagct tgagaagcag gtcggcgtcg tcatcccgct
    10921 gcccgcgggc cagcaaacgg gcaccactgg cagcgaagcg gccgatctcg cggcgaaagt
    10981 aagacagcac ggccagcagc gtgcccgcat gcacggctat gtcgaacgcc agcccctgat
    11041 cgggccactc ggtgagcaat gccgggagga tgagatgagc cgagctggag atcggcagga
    11101 actccgtgat gccctgcacc agggcaagga tgatgatctg cagccagtcc acggctgcgc
    11161 agtctaacgg caagcatccc ctttcgcgac cgcagcgcga ctgcgatgca gcagcgtgct
    11221 aaggttctcc gatggagatc ccgctgttcc ccctgcgcgt cgttcttttc gacggcggcc
    11281 ggttgccttt gcagatattc gaaacccgct acctggacat ggtgagccgc tgcacgcggg
    11341 agtcgatccc cttcggcgtc gtgctcatac ggggtgcggg cagcgacgct cgcctggatc
    11401 cgctcgccaa ccagcccgcg attttcgagg taggcaccga agcccacatc gtcgacttca
    11461 atcagctgcc gaacggacgg ctcggcatca tcgtccgggg tggccgcaaa ttccgcgtgg
    11521 tatcgacccg ggagcaggcc gaccacctgc tgctcggaac ggtggacttc ctgccggccg
    11581 agcccgatag caccgtgcca tcagagcacg cccctctggt cgacatcctc aaagagctgg
    11641 tgaaacatcc cggggtgcag aagctcgggt tggaaatcga cttcgccgac gcgcggtcgg
    11701 tgggcgcccg cctggccgag ctgcttccca tagaacccga gatcaagcag agcctgctgc
    11761 agctgcagct gccccgggaa cgcctccagg agctgacccg gctggtcaac aaattccggg
    11821 actagaaggg aatgtcgtcg tcgaagtcgt cgtccgccag cgttggcgcg ttctcggacg
    11881 ctgcgggcgc agctgattcg aacccgccgc cctggccgcc ctgaccgccc ccgcggctgt
    11941 cgagcatctg caggtccctc gccatgacct cagtccggta gcgtttctga ccgtcctgct
    12001 cccagctgct ggtacgaagg ctgccctcga tgtaaacctt cgagcccttg cgcagatact
    12061 cacccgcgat ctcggcaagg cgcgcgaagc acaccacgtt gtgccactca gtgcgttcct
    12121 gctgctcgcc cgactggcgg tcccgccagt tttccgtggt ggcgacgctg aagctggtga
    12181 ccgcattgcc gccctgggag tagcgcgtct caggatccct gcccagattg ccgatgagaa
    12241 tgactttgtt gataccccgc gccatagcct ttttcccgtt gcctcttacc cgttcaaatg
    12301 cccagaaaac tccaggcccg cagtctagca cggcgccgtg aaaagtccgt aagcgatggt
    12361 ccagcttgcc cctgagtagg gatcggttta tgttgccggg tttccccact tccggggtca
    12421 atcagcgcaa gctagggtcg gcgagccgcg ctggcagggc ataaggcacg cgcattggac
    12481 agcatcgcgg ttcgcggggc acgcacccac aatcttaaaa acgtcaatct ggacataccc
    12541 cgcgatcggc tcgtggtgat cactgggcta tcgggatccg gaaagtcctc gcttgccttc
    12601 gacaccctct atgcggaggg gcagcgccgc tacgtggagt ccctctcggc ctacgcccgc
    12661 cagttcctgt cgatgatgga gaagccggac gtcgaccata tcgagggcct gtccccggcc
    12721 atctccatcg agcagaagtc cacctcgcac aatccccgct ccaccgtggg tacgatcacc
    12781 gagatctatg actatctgcg gctgctgtac gcgagggtcg gcgagccgcg ttgccccaac
    12841 cacgacctgc cccttgacgc ccagacggtc agccagatgg tggaccaggt tctcggcctg
    12901 ccggaggaca agcgggtaat ggtcctcgcg cccgtggtca ccgaacgtaa gggcgagcac
    12961 ctgcatgtct ttcaggaact catggcaaac ggcttcatca gagcccggat cgacggcatc
    13021 gtggtcgatc tcgaccaccc gccaaacctg gagaagaaca aaaagcacac cgtggaagcc
    13081 gtcatcgacc ggctgcgtat ccgggcggat gcgcagcagc ggctcgctga atccttcgaa
    13141 accgctctag atctcgccga ggggatcgcg aaggtggcgt ccatggacga tgccgacgag
    13201 ccggaactcg ttttctcggc aaggttcgca tgtcccgcct gcgggtacag catatccgag
    13261 ctcgagcccc gcatgttctc cttcaacaac cccgccggcg cctgccaggc ttgcgacggc
    13321 cttggggtga agcagttttt cgatcccggc ctggtggtcc aggacgagga gctgacgctg
    13381 gccgaaggcg ccatacgcgg ctgggaccgg cgcaacgttt actacttcca catgctctcc
    13441 tctcttgcag cccactacgg cttcgacgtg gagacgcctt tccgaaagct gcccaaaaag
    13501 catcgggacg caatcctcta cggcagcggc agagagcgga tcgatttcag ctacgccaac
    13561 gaccgcggcg acgtgatcca tcgccgccac cggtttgagg gcgtgatacc gaacatggag
    13621 cgccgttatc acgagaccga ctccagcctg gtccgagaga acctgcagaa gtaccttcgg
    13681 gtcaggagct gccccgactg cggcggcacg cggctgcgcg aggcgtcgcg caacgtcttc
    13741 atcgacggcg tcagcctgcc ggagataacg tccgggtccg tggagggctc cctggcctac
    13801 ttcgacgagc tcaagctgtc ggggcgacgg ggagagatcg ccgacaagat cctcaaggag
    13861 attcgcgccc gtctgcggtt tctggtcgac gttgggctca actatctcac cctggaccgc
    13921 agcgccgaga cgctttcggg gggggaagcc cagcgcatcc gcctggcaag ccagatcggc
    13981 gccggcctgg tcggcgtcat gtacatcctc gacgagcctt ccatcggcct gcaccagcgg
    14041 gacaacgccc ggctgctcaa gactctggag caccttcgcg atctgggaaa cacggtgctg
    14101 gtggtagagc acgacgagga ggcgatccgc tccgcagacc acatcatcga catcggacca
    14161 ggggccggcg tgcacggcgg ctgcgtggtt gccgccggca gtctcgagaa gatcctgaaa
    14221 tccaggcgct ccatcaccgg ccagtatctc tccggtagga agaagattcc cgtgcctgaa
    14281 aagcggacgg cgttcgtcga ggaggagacc atccgcatca tcggcgccac cggcaacaat
    14341 cttcagtccg tgtcggtcga gataccgtgc ggcctgctga cctgcgtaac cggcgtttcc
    14401 ggctcgggca agtcgacgct gatcaaccac actctctacc ccgtggcagc taccagactc
    14461 aacaaagcaa ccacgctgac gccggcaccc gtggaggagg ttctgggtct gaatcatgtc
    14521 gacaaggtgg tcgacatcga ccagagcccc atcggccgca cgccgcgctc caatccggca
    14581 acgtatacgg gtctgttcac gccgatccgt gagctcttcg ctcagaccca ggaggccagg
    14641 acccggggtt acaagcccgg ccgcttcagc ttcaacgtca agggcgg
//
LOCUS       DBNHLM010000253         9036 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1353203, whole genome shotgun sequence.
ACCESSION   DBNHLM010000253 DBNHLM010000000
VERSION     DBNHLM010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9036)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9036)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9036
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1353203"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="AC16QF_11875"
     CDS             complement(<1..834)
                     /locus_tag="AC16QF_11875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6449301.1"
                     /translation="MTDFGVLIFPTAYSISPSDLARAAEARGFESIFFPEHTHIPACR
                     TTPWPGGRELPREYWNTYDPYVGLAMAAAVTKTLKVGTGITLVTERDPIVLAKQVASL
                     DRLSGGRVLLGIGAGWNVEEMRHHGVAFDERWAVLRERVLAMRAIWTEQAPEFHGEHV
                     DFEPLWSEPKPLQAGGPKILMGASSRWTWARIAEYADGWFPIHQDPARASAQGAVDYP
                     QGIQATREAWQAAGRQGEPDFSIFGMGPDRGRTEEMIRLGFNRVIFGLPSAGADTVLP
                     LL"
     gene            complement(850..2430)
                     /locus_tag="AC16QF_11880"
     CDS             complement(850..2430)
                     /locus_tag="AC16QF_11880"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6449302.1"
                     /translation="MLEVPVLIVGGGPVGLAAALECAYFGVPAMLVERHSGTTWHPKA
                     RNLNSRTMEIMRRLGLEPELQAVGLPEAWTRQIVYADTLSGAELGRMGTGGFESRSSA
                     ASPCAAVLSSQDVFEPIWRGRAEQQPLLDVRYGHQAEILDVDAAGAVVRLTGARGGDV
                     RARYLLAADGAGGAIRQGLGIGLEGPENLGHFINVYYRANLDRVVAHRPAVLYFTANE
                     AGRGVFQPLDGRKRWLSQIAYDGAADTRAGFDGERCAQWIRNAAGEPDLKVEILGISH
                     WTMNATVAERYRQGAAFLVGDAAHQLPPTGGFGLNTGIQDVHNLVWKLAYDLRDWSDA
                     GLVDTYEAERKPVARFNADRAMDNSRAVARIQRAVADGEAAAGEVARSRRYGNFRGLE
                     LGYSYRSAAVIDDGSPAPEPEDAVEDYVPCARPGHRMPHMWLEGGALSTLDLVQRRFL
                     VLSDSARWLEAASQHNADCRVLPGNGGFLQLFGLEPGGAVLVRPDGHVAARFAEPPGF
                     PGGALASALRDLLNTTTT"
     gene            complement(2499..3152)
                     /locus_tag="AC16QF_11885"
     CDS             complement(2499..3152)
                     /locus_tag="AC16QF_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR6449303.1"
                     /translation="MPTVATARQNENRKEAKRAKVRWYTPFLFVLHSLAIVGPVLLAA
                     GFAHRVEDAAYRTFAFAVPAPENSYVTWNSEVSEFGTKVSQAFGVRPSTATEFAGWIL
                     EASTRQELEPELLASLVLTESSFRKNVRSHVGAIGPAQVRPEYWSNFCGIANLHDPAE
                     NIYCGAQVLSHLRDRCGDEGCALHAYNVGLYAPRHKAGHRYVAKIDHYREQLLNLPL"
     gene            complement(3410..4417)
                     /locus_tag="AC16QF_11890"
     CDS             complement(3410..4417)
                     /locus_tag="AC16QF_11890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6449304.1"
                     /translation="MKVDGGVGWELDLVGAQAQELEEMGYSGILSAETSHDPFFPLLV
                     AAQNTERVDLMTSIAVAFARSPMTLANIGHDLNAVSKGRFVLGLGSQIRAHIMKRFSM
                     PWSHPAARMREFILAMRAIWANWHDGEPLAFTGKFYTHTLMTPFFTPTDTEYGPPRVF
                     LAAVGPMMTEVAGEVADGVIVHAFTTEKYLRETTLPALERGFAKAGKTRADFEISYPV
                     FVVTGENEEELAQAKVATARQIAFYGSTPAYKPVLESIGVGEMQGELNAMSKQGRWEE
                     MGHLISDDVLEAFAVVGEPQQIAGQIISRYGDIVDRTSAAYAHISKEHRQEIIAELRL
                     A"
     gene            complement(4436..5113)
                     /locus_tag="AC16QF_11895"
     CDS             complement(4436..5113)
                     /locus_tag="AC16QF_11895"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6449305.1"
                     /translation="MKRLYLIRHGQTEWNVAKRMQGRLDSPLTSAGRIQAAVHGRLLS
                     EIASLEQLVVSPSGRTRETACILNSHVGAPMRHDEALMERDCGEWSGLTIEEIEQHFP
                     EAWEARREDPFYHRPPGGENHEDLVQRAGPLLAEFLAGDVTEVGLVTHGVMSRAIVTH
                     LLELTPVEAVGIRHPNDLVYRLDICATGVQVSHFLAGDGPRAGLLRQNENETISGPAR
                     AGERRDR"
     gene            complement(5147..5989)
                     /locus_tag="AC16QF_11900"
     CDS             complement(5147..5989)
                     /locus_tag="AC16QF_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6449306.1"
                     /translation="MEYQKIIVDEPRPMVRRITLNRPEKRNPLSNELRSEMFHALESA
                     DQDDGVRVTIIRGAGPCFSAGYDLKSDTSQNPPFYTAGGLGHWPRHVVEGFFRMWDMA
                     KPIIAQVHGYCLAGGTELATACDLVYVAEDAKIGYPVVRTMSPPDNQFYPWIVGLRRA
                     MELMLTGDHMSGVEAAESGFANRAFPAEELEERVLAIAERVALTPTDLQQFNKRAVHR
                     QMDAMGIRAGIRAGTEMQQLAFFTDTTRAYFKEMSKGLTHALSKRDEAFGDYRTSASG
                     KAED"
     gene            complement(6031..7032)
                     /locus_tag="AC16QF_11905"
     CDS             complement(6031..7032)
                     /locus_tag="AC16QF_11905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6449307.1"
                     /translation="MALAKRMAVTLPAGPKIEHTVARLQWAEDNGFADAWFGDSGAPD
                     SLTQVAAVAHHTSSIRIGVAVTPVYTRTPAVLAATANVLEQVLPGRFVMGLGSSSQTI
                     MGQWNGIPLDRPLTRVRETAILVRSMLTGEKSNFDGETLTSHGYRQPPLDHPPPIYLA
                     ALRPKMIEMAAEVGDGVIFNLWPKSVLPKMMEHVRIGAERAGKNWEDVEVVNRAMVLC
                     TNDKQAGRNLFRAAFAPYYATPVYNAFLAWAGHADAAGAITEGWAAKDRAMTTGALSD
                     ELIDEIAIIGDEDEIRARIQADADGGVHTHIVAPLAASPEDVERTFAAFTADGFSLK"
     gene            7134..8177
                     /locus_tag="AC16QF_11910"
     CDS             7134..8177
                     /locus_tag="AC16QF_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6449308.1"
                     /translation="MSDVELTPVREMHRFDEDALDRYLQAHVNGYRGPLTVRQFEGGQ
                     SNPTFQLEAGGRLYVLRKQPPGELLPSAHQVDREYRVMKALEQTEVPVPLMKCMCEDA
                     GVIGTKFYVMEMVNGRVFAETLLPSLTPPERRAIYLDLARVLAKLHSVDPAAVGLADF
                     GRPGNYYERQIGRWTKQYEASKTEQIDAMDRLMAWLPERIPEQGETVIVHGDYRLGNA
                     LIHPTQPRIVAVLDWELSTLGDALADLGYICQDYHGDSYNDAGLSGADLGALGIPTED
                     ELVAEYCRHAGREHIDNWPFYVIYNMFRSASIIQGVYKRGLDGNASSEKALEYKDAAR
                     IRSEQAWALVESL"
     gene            complement(8179..>9036)
                     /locus_tag="AC16QF_11915"
     CDS             complement(8179..>9036)
                     /locus_tag="AC16QF_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6449309.1"
                     /translation="IMQMPARTPAMTAMTAMSLAELSGGRFICGVGASGPQVVEGWHG
                     VPYGLPVTRLKEYVQIMKRIFAREAPLEFEGRMYQLPYQGPGATGLGKPLKSILHCEE
                     DIPIYAATITPKGVQAAAEVADGFFPVWMDPEQYHVFEEPVAKGFASAGGDKDLTRFD
                     IAPFVTVVMGDDVEQCMMPIRGSMALYIGGMGARGKNFYNDYAKRLGFEEAAVRIQDL
                     YLEGRKDEAMAAVPAELIDACHLVGPADRIRERLQRWQAAGRQGHVSSMLLGCQQPEA
                     LALVAETVL"
BASE COUNT         1569 a         3049 c         2838 g         1580 t
ORIGIN      
        1 gagcagcggc aacaccgtat ccgcgcccgc cgagggcaac ccgaatatca cccggttgaa
       61 acccagccgg atcatctctt cggtgcgccc ccggtcaggc cccattccga agatgctgaa
      121 gtcgggctcc ccctgccggc cggctgcctg ccacgcctcg cgggtggcct gtatcccctg
      181 cgggtagtcc acggctccct gggccgacgc ccgggcggga tcctggtgaa tggggaacca
      241 gccgtccgcg tactctgcga tccgcgccca cgtccaccgg gaggaagcgc ccatcaggat
      301 cttcgggcca cccgcctgca gcggcttcgg ctcggaccac agaggctcga agtcgacgtg
      361 ctccccatga aactccggcg cctgctccgt ccatatggcc cgcatggcaa gcacccgctc
      421 cctgagcacg gcccaacgct cgtcgaaagc gaccccgtga tgacgcatct cctcaacgtt
      481 ccaaccggca ccgatgccga gcagcacccg gcccccggac agacgatcca gagaagccac
      541 ctgcttggcg agcacgatgg gatcccgttc ggtaaccagc gtgatccccg tacccacctt
      601 caacgtcttc gtgaccgctg cggccatggc gagccccaca taggggtcgt aggtattcca
      661 gtactcgcgc ggcagctccc tgcctcccgg ccagggcgtg gtccggcagg ccggtatgtg
      721 ggtatgctcc ggaaagaaaa tcgactcgaa accgcgggcc tcggccgcgc gcgccaggtc
      781 cgaaggggag atggagtagg cggtggggaa gatgagcacg ccgaagtccg tcatctggca
      841 atctccttat caggttgtgg tggtgttgag cagatctctg agcgccgacg ccagcgcccc
      901 gccgggaaag ccgggaggct cggcgaatcg ggccgccacg tggccgtccg ggcgcaccag
      961 caccgcgcca cccggctcga gcccgaacag ctggagaaaa ccgccgttgc cggggagcac
     1021 ccgacagtcg gcgttgtgct gagaggccgc ttccagccag cgcgccgagt ccgaaagcac
     1081 cagaaagcgc cgttgcacca ggtccagggt ggacagggcg cctccctcca gccacatgtg
     1141 gggcatgcgg tgaccggggc gggcacaggg cacgtagtcc tccacggcat cctccggctc
     1201 gggcgccggg ctgccgtcgt cgatcaccgc tgccgatcgg tagctgtacc ccagctccag
     1261 accccggaaa ttgccatagc gccgcgaccg cgccacctcg cccgccgcgg cctcaccgtc
     1321 ggcgaccgcc cgctggatgc gtgccacggc gcgcgagttg tccatggccc gatccgcgtt
     1381 gaaccgggcc accggctttc gctccgcttc gtaggtgtcg acgagaccgg cgtccgacca
     1441 gtccctcaag tcgtaggcca gtttccatac gaggttgtgc acgtcctgaa tgcccgtgtt
     1501 caggccgaat ccgccggtgg gcggcagctg gtgcgcggcg tctccgacga gaaacgccgc
     1561 gccctgccgg tatcgttcgg ccaccgtcgc gttcatggtc cagtgggata tgccaaggat
     1621 ctcgaccttg aggtcgggtt cccccgcggc gttcctaatc cattgtgcgc atcgctcgcc
     1681 atcgaacccg gcgcgagtgt ccgccgcgcc gtcgtaggca atctgcgaca gccagcgctt
     1741 gcgcccgtcc aggggctgaa acacgccgcg ccccgcttcg ttggccgtga agtaaagcac
     1801 cgccgggcga tgagccacca ctcggtcgag attggctcga tagtagacgt tgatgaagtg
     1861 gccgagattc tcgggccctt ccagcccgat gcccagccct tgccggatcg caccaccggc
     1921 cccgtccgcc gccaggaggt agcgggcgcg aacatcgccc cctctggctc cggtgaggcg
     1981 cacgacggct cctgcggcgt ccacgtcgag gatttccgcc tgatgcccgt agcgcacgtc
     2041 caggagcggt tgctgctccg cccgccctcg ccatatgggc tcgaacacgt cctgtgagct
     2101 caaaaccgcc gcgcacgggc tggctgccga gcttcggctc tcgaaccccc cggtgcccat
     2161 gcgtccgagc tcggcaccgc tgagcgtatc ggcatagacg atctggcgcg tccaggcctc
     2221 gggaaggcca acggcctgca actcgggctc caagccaagc cgacgcatga tctccatggt
     2281 ccgggagttg aggttacgcg ccttgggatg ccaggtggtg ccggaatgcc gctcgacgag
     2341 catcgccggc acgccgaaat aggcgcactc gagcgctgcg gccagcccca cggggccacc
     2401 tccgacaatg agaacgggaa cttcgagcaa cgcgcatccc ctggggcgcc agccaagagg
     2461 gtacgccctt ccgggcgggc taggctagag gcgtcagatc aaagaggcag gttgaggagc
     2521 tgctcgcgat agtggtcgat cttggcgacg tagcggtgtc cggccttgtg ccgcggggcg
     2581 tagaggccaa cgttgtaggc atgcagcgcg cacccttcgt caccgcagcg gtcccgcaga
     2641 tgggaaagca cctgggcacc acagtagatg ttctccgcgg ggtcgtggag gttggcaatg
     2701 ccgcagaagt tgctccagta ctccggccgc acctgggccg ggccgatggc ccccacgtgg
     2761 gagcggacgt tctttcgaaa cgagctctcg gtcagcacca ggctggccag caattcaggc
     2821 tcgagttcct gccgtgtaga tgcctccagg atccaccctg caaactccgt ggccgtgctc
     2881 ggccgcacgc caaacgcctg ggacaccttg gtgccgaact cgctcacctc ggaattccag
     2941 gtcacgtagg agttttccgg cgctggcacc gcaaacgcga aagtccggta ggcggcgtcc
     3001 tcgacgcggt gcgcaaagcc ggcggccagc agcaccgggc caacgatagc caggctgtgc
     3061 agcacgaaga ggaagggcgt gtaccagcgc acttttgcgc gcttcgcctc cttccggttc
     3121 tcgttctgcc ttgccgttgc caccgttggc acctcgtctc gtgttcatat cgactgtgta
     3181 cggctgcgca ttctaaagac ctgaaatgcc acaatgaacc caaatttcgt gcgaagtcgt
     3241 tggcaggttg ccaccgaaac gttgggagaa tgcaaaaatg cgggccgtgc acggactttt
     3301 gtgaggaatt ccttaccgtc cacgccccgt cacgcttgaa tcgcgaatca gaacaccgct
     3361 cgattacttt ttatacctaa aagtaatcaa tttttcattt tttagaacat tatgccaatc
     3421 tcagctcggc gatgatctcc tgacggtgtt ctttggaaat gtgcgcatag gcagccgagg
     3481 tgcgatcgac gatgtcgccg tagcgagaga tgatctgacc ggcgatctgc tgcggctcgc
     3541 ccaccacggc gaacgcctcg agcacgtcgt cgctgatgag gtgccccatc tcttcccacc
     3601 ggccctgctt ggacatggcg ttcagctcgc cctgcatctc gccgacgccg atgctttcca
     3661 gcaccggctt gtaggcgggc gtggacccat agaaggcgat ctgacgcgcc gtggccacct
     3721 tcgcttgcgc cagctcttcc tcgttctccc cggtcacgac gaataccgga tagctgatct
     3781 cgaagtcggc gcgcgtcttg cccgccttgg caaacccccg ctccagagcc ggcagcgtgg
     3841 tttcgcgcag gtacttctcc gtggtgaacg cgtgaactat cacgccgtcg gcgacctcgc
     3901 cggcgacctc ggtcatcatg ggcccgacgg cggcgagaaa gacgcgcggc ggtccgtatt
     3961 cggtgtccgt gggggtgaag aacggcgtca tgagcgtgtg ggtgtagaac ttgcccgtga
     4021 acgcgagggg ttcgccgtca tgccagttcg cccagattgc ccgcatggcg agaatgaatt
     4081 cgcgcatgcg cgccgccggg tgagaccagg gcatgctgaa tcgcttcatg atgtgcgccc
     4141 ggatctgcga gccgaggccc agcacgaaac ggcctttgga aacggcgttc agatcgtggc
     4201 cgatgttggc cagagtcatg ggcgatcggg caaaagccac cgcaatcgag gtcatcaggt
     4261 cgacccgctc cgtgttctgc gccgccacga gcagcgggaa gaacgggtcg tggctggttt
     4321 cagccgacag gattcccgaa tagcccatct cctcgagctc ctgggcctga gcgcccacca
     4381 gatcgagttc ccaacccaca ccgccgtcga ctttcatcgt cttcccccag tttttttatc
     4441 ggtcccgccg ctctccggcc cgcgcggggc ccgagattgt ttcattctcg ttttggcgaa
     4501 gcaatcccgc ccgcggaccg tctcccgcga gaaagtggct gacctggaca ccggttgcgc
     4561 agatgtccag ccgatagacc agatcgttgg ggtgacggat cccgaccgct tccacgggcg
     4621 tcagctcgag cagatgggtc acgatcgccc gggacatgac gccgtgggtc accaggccca
     4681 cctcggtaac gtctccggcc agaaactccg ccagcaacgg gcccgcccgc tgcaccagat
     4741 cctcgtgatt ctcgccgcca ggtggccggt gataaaaggg atcctcccgc cgggcctccc
     4801 aggcctccgg aaagtgctgc tcgatctctt cgatggtgag ccccgaccac tcaccgcagt
     4861 cgcgttccat gagggcttcg tcatgacgca tcggcgcccc aacgtgggag ttcagtatgc
     4921 aggcggtttc gcgcgtccgt ccggacgggg acaccaccag ctgctccaga ctggcgatct
     4981 cgctcagcag acgaccgtga acggccgcct ggatccgacc ggccgaggtc aggggcgaat
     5041 cgaggcgtcc ctgcatgcgc tttgccacat tccactcagt ctggccgtga cggatcagat
     5101 agagccgctt catgattccc gttggagatc ccggcaccgg agcccgtcag tcctcggcct
     5161 tgccggacgc ggaggtccgg taatctccga aagcctcatc gcgtttcgac aaagcgtggg
     5221 tcagcccctt gctcatctct ttgaagtacg cacgcgtggt gtccgtgaag aaggcgagct
     5281 gctgcatctc cgtgccggca cgaatgccgg cccggattcc catggcgtcc atctgccgat
     5341 gcacggcgcg tttgttgaat tgctgcagat ccgtgggcgt gagggcgacg cgctcggcta
     5401 tcgccagaac ccgctcctcc agctcctcgg cgggaaaggc acggttggca aagccgcttt
     5461 ctgccgcttc gacccccgac atgtgatcac cggtgagcat gagctccatg gcccgcctga
     5521 ggccgacgat ccaggggtag aactgattgt ccgggggcga catggtgcgc acgaccggat
     5581 agccgatctt ggcatcctcc gccacgtaaa ccaggtcgca ggccgtggca agctcggtac
     5641 cgccggccag gcagtatccg tgcacctggg caatgatcgg cttggccatg tcccacatgc
     5701 ggaagaatcc ttccaccacg tgccgcggcc agtgaccaag gccgccggcc gtgtagaacg
     5761 gcgggttctg gctggtatcg ctcttcagat cgtagcccgc cgagaagcac ggaccggccc
     5821 cgcgtatgat ggtgacccga accccatcgt cctgatcggc gctttccagc gcgtggaaca
     5881 tctccgagcg cagctcgttg gagagcgggt tgcgcttctc gggccggttg agcgttatgc
     5941 ggcggaccat gggccgcggc tcgtcgacga tgatcttctg gtattccatg atctcctcac
     6001 tgtcttcgga tcgacggacc cggagggtgc ctatttcaaa ctgaacccgt ctgccgtgaa
     6061 cgcggcgaac gttcgctcca cgtcttcggg cgaggcggcc aggggagcga cgatgtgggt
     6121 gtggacgccg ccgtccgcat ccgcctgtat gcgcgcgcgg atctcatcct cgtcgccgat
     6181 gatggcgatc tcgtcgatga gctcatcgct cagggcgccg gtcgtcatcg cccggtcttt
     6241 cgccgcccag ccctcggtaa tggcgccggc ggcatcggca tgacccgccc aggcaagaaa
     6301 cgcgttgtaa acgggggtgg cgtaataggg cgcaaaggca gcccggaaca ggttccggcc
     6361 cgcctgcttg tcgttcgtgc acagaaccat tgcacggttg acgacctcta cgtcttccca
     6421 gttctttccg gcgcgctcgg cgccgatgcg aacgtgctcc atcatcttcg gcagcacgct
     6481 cttaggccac agattgaaga tcacaccgtc cccgacctcg gccgccatct cgatcatctt
     6541 cgggcgcagg gcggccagat agatgggcgg cggatggtcg agcggtggct ggcggtaacc
     6601 gtggctcgtc aacgtctcgc cgtcgaagtt gctcttctcg ccggtcagca tggaacgcac
     6661 caggattgcc gtctcccgga cccgggtcag gggccgatcc agcggaatcc cgttccattg
     6721 acccatgatg gtctggctcg aggagcccag gcccatgacg aaacgtccgg gcaggacctg
     6781 ctctagcacg ttggccgtcg cggcgagcac cgcaggcgtg cgcgtataca ccggcgtgac
     6841 ggccacgccg atgcgtatgc tgctggtgtg atgggcaacc gccgccacct gagtgaggct
     6901 gtccggcgcg cccgagtcgc cgaaccaggc atccgcgaaa ccgttgtcct cggcccactg
     6961 caagcgcgcg acggtgtgct cgattttggg tccggcgggc aaggtgaccg ccattctctt
     7021 cgccaatgcc atgctgcctc cccaggtgcg gccttgcgga aaacccacag cctcgcattt
     7081 tgcggccgac gagatcaagc cgtatccttg cccgtcgctt cgggagcaaa gcaatgagcg
     7141 atgtagagct gacgccggtg cgcgagatgc accgattcga cgaggacgcg ctggaccgtt
     7201 acctgcaggc ccacgtgaac ggctaccgcg ggcccctgac ggtgcgtcag ttcgagggcg
     7261 gacagtcgaa cccgaccttt cagctcgaag cgggtggcag gctctacgtt ctgcgcaagc
     7321 agcctccggg tgagctgctg ccgtcggccc accaggtgga ccgcgagtac cgggtgatga
     7381 aagcgctgga gcagaccgaa gtgcccgttc ccctgatgaa gtgcatgtgc gaggacgcag
     7441 gcgtgatcgg cacgaagttc tacgtgatgg agatggtgaa cggacgcgtg ttcgcagaga
     7501 cgctgcttcc gtctctgacc ccgcccgaac ggcgcgccat ctacttagac ctcgcccgag
     7561 ttctggcaaa gctccacagc gtggaccccg cagcggtggg gctcgccgac ttcggccgcc
     7621 ccggcaacta ctacgagcgg cagatcggtc gctggaccaa acagtacgag gcatcgaaga
     7681 ccgagcagat cgacgccatg gacaggctca tggcgtggct tcccgaacgc atcccggaac
     7741 agggggagac cgtcatcgtg cacggcgact accggctggg caacgcgctg atccacccta
     7801 cgcaaccccg catcgtggcg gttctggatt gggaactgtc gacgctgggg gacgcgctcg
     7861 ccgatctcgg ctacatctgc caggactacc acggcgattc ctacaacgat gcgggtcttt
     7921 ccggcgccga cctgggcgcg ctcggcatcc ccaccgagga cgagctcgtg gccgagtact
     7981 gccggcacgc gggccgcgag cacatcgaca actggccgtt ttacgtgatc tacaacatgt
     8041 tccgctcggc gagcatcatt cagggcgtgt acaagcgcgg cctggacggc aacgccagct
     8101 ccgagaaagc gctggagtac aaggacgcgg cccgcatccg atcggagcag gcctgggccc
     8161 tggtggaatc gctctaagct agaggacggt ttccgccacc agcgccagcg cctccggctg
     8221 ctggcaaccc agcagcatgc tggaaacatg cccctgacgc cccgccgcct gccagcgctg
     8281 caaccgctcg cgaatccgat ccgccggccc caccaggtga caggcgtcga tgagctccgc
     8341 gggcaccgcg gccatggctt cgtccttgcg cccttcgagg tacaggtcct ggatcctgac
     8401 cgcagcctcc tcgaaaccca gccgcttggc gtagtcgttg tagaagttct tgccgcgggc
     8461 gcccatgccg ccgatgtaga gcgccatgct gccgcgtatg ggcatcatgc actgctcgac
     8521 gtcgtcgccc atcaccacgg tgacgaaggg tgcgatgtcg aaccgggtca gatccttgtc
     8581 cccgccagcg ctggcgaagc ccttggcaac cggctcttcg aagacgtggt actgctcggg
     8641 atccatccac acggggaaga agccgtccgc cacctccgcg gctgcctgca cgcctttggg
     8701 cgttatggtc gcggcgtaga tggggatgtc ctcctcgcag tgaagaatgc tcttcagagg
     8761 tttgcccaga cccgtggcac ccggaccctg ataaggcagc tgatacatcc gcccctcgaa
     8821 ctcgagcggc gcctcccggg caaagatgcg cttcatgatc tgcacgtatt ccttcaggcg
     8881 cgtcaccgga agcccgtagg gaacaccgtg ccagccttcc acgacctggg gtccggaagc
     8941 accgacgccg caaatgaaac gaccgcccga cagctcggca aggctcatcg ccgtcatcgc
     9001 cgtcatggca ggcgtgcgtg ccggcatctg catgat
//
LOCUS       DBNHLM010000254        10279 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1472806, whole genome shotgun sequence.
ACCESSION   DBNHLM010000254 DBNHLM010000000
VERSION     DBNHLM010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10279
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1472806"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1978)
                     /locus_tag="AC16QF_11920"
     CDS             complement(<1..1978)
                     /locus_tag="AC16QF_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6449310.1"
                     /translation="MIARIIDGAIGERLIVLLGALILAGAGTWAYLTVPVDAIPDLSD
                     VQVIVFTDYPGQAPQVVEDQVTYPLTTAMLAVPKARVVRGYSFFGLSFVYVIFEDGTD
                     MYWARSRVLESLNYVSGRLPAGVNPALGPDATGVGWIYEYALVDRSGRHDLAQLRSLQ
                     DWYLRYPLQTVPGVAEVASVGGFVKQYQVEVDPNALMALGIPLSKIKQAIQRSNNDVG
                     GRLLEISETEYMVRGLGYIRSLEDLGSIPVAADPGGTPIRLGDVARIQIGPELRRGLV
                     DLDGDGEVVGGIVVMRYGENARATIEGVRQRLDELRAGLPEGVEIVTVYDRGDLIDRA
                     VDTLSRSLLIEIVIVSLVVIVFLAHARSAMVAVLILPLGVLIAFLLMRWHGLNANIMS
                     LGGIAITIGAMVDGAIVMVENAHKHMARDAERKGAELSSAERWQAVGAAAREVGPALF
                     FSLLVITVAFLPVFSMQAQEGRLFSPLAFTATYAMAGAAILAITLVPVLMGYLVRGRI
                     RPEESNPINRWLHRLHTPLLGLAMRWRWATVVMAGLLLALTWVPFSRLGSEFMPPLDE
                     GDILYMPTTFPGVSISKARELLQQTDRILREFPEVGQVFGKAGRAETATDPAPLAMFE
                     TTIRLKPRREWPDPTKTTRELIDEMDRELKLPGLA"
     gene            complement(1975..3318)
                     /locus_tag="AC16QF_11925"
     CDS             complement(1975..3318)
                     /locus_tag="AC16QF_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6449311.1"
                     /translation="MGKKFLLLVIVALAGGVGAGYFFAELSAGKPSETAPLEPEGRAR
                     EPIFYRNPMNPSVTSAVPAKDSMGMDYIPVYADEEAAGPAGTVSIDPIVVQNIGVRTA
                     RAQRAALSHTVRALGRIDFDERSIARLHPKVEGWVEEMYVDTTGAQVSEDTMLLSVYS
                     PRLVASQQEYVLALRNYESLNDSPIPDISRGAADLVASSRARLELLDVPEHQIVELEK
                     SRVPQKSLHIHSPAEGVVLKIGARQGQHVTPMTELYVIADLSRVWVYADVFEHDLPWV
                     RVGDAVEMTLGALPGETFGGLLDYIYPYAEAKTRTVKARLVFDNPDRLLRPEMFADVL
                     IRTSTEPNALVIPAEAVVRSGLTNQVFVVRAPGRFEPRVVELGVESEGRVSVLEGLAE
                     GEEVVTSGQFLIDSESKLREAAAKMMARPGDEASSPDGVGDHDHHHGGHDRGASP"
     gene            complement(3311..4606)
                     /locus_tag="AC16QF_11930"
     CDS             complement(3311..4606)
                     /locus_tag="AC16QF_11930"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR6449312.1"
                     /translation="MMRTWLVASLAVAGVAQGSEDGGDLLRTGLTLELALERAVRTNP
                     NLAGIQSRYEAMAAIPSQVGTLPDPVLSFGAVNFPTDTFHVGQEPMTQLQVGISQTIP
                     FPGKRALREAASSHEAEAARSSVAETRLRLIRDVSFNWWTLHYLDRAQEIVQRNQELL
                     RQFVQIAKTRYEVGGGLQQDVLLAQLELSKLLDQAIQLNGARDVEAARLNRLLDQPAN
                     QPIELPRTPVGVFPDLVAQQELYQRGAESRPLLKRLRSEIDAAESRLELARKDYYPDF
                     TVGAAYGARSGENPMPGGGDRTDLLTLRVSVNLPIYQSRKRAQAVSQRRSEMDRHRYA
                     LQDEVNAMRAEISQAVADYRRAREQLVLLDSGIIPQARQTVQSMLAGYQVGEVDFLNL
                     VRSQVTLYNYETLYWKAVTLANQSVARLSAAVGEEVRGG"
     gene            4781..5197
                     /locus_tag="AC16QF_11935"
     CDS             4781..5197
                     /locus_tag="AC16QF_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449313.1"
                     /translation="MTAQPVGDEPFRTGSQWRFQSTPGEKGRRSRGGTLLETLVAAIT
                     VRGPVFDGHLPMVAMMLIVTGVLGTMTHMMPGCTRRCFAGPLAGTSQRRERRRQHKRY
                     QQMRNKGIHEAHERRLSRDPPLHNAYTVPLPTGSSD"
     gene            5292..6194
                     /locus_tag="AC16QF_11940"
     CDS             5292..6194
                     /locus_tag="AC16QF_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR6449314.1"
                     /translation="MTTEQLLRFATYASVATATVLIAAKLTAWFATDSVSVLASLVDS
                     VMDVGASLINLFAVRYSLMPADQEHRFGHGKAEALAGLGQATFIAGSAIFLILHAVDR
                     LMDPVTIEHVAAGSAVMGFAIVMTLVLLTIQRHVIRRTGSTAIRADALHYATDLATNA
                     ATLLALLLAAYGFSGLDPLFGLAIAAYILYSASRIGRDAVSLLMDRELPQAEREEIKR
                     VVDGTPGVLGVHGLRTWQSGQRKVIQMHLELDPDLPLREAHRIAVEVERRLLEHEPEA
                     DITIHQDPAGQPSHVAHNPGDAFS"
     gene            6260..6721
                     /locus_tag="AC16QF_11945"
     CDS             6260..6721
                     /locus_tag="AC16QF_11945"
                     /inference="COORDINATES: protein motif:HMM:NF039797.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HIR6449315.1"
                     /translation="MSTRILMLSMLLLAGCASAPEAEQAQQQREEDIATILNEPLDPS
                     EYGEAKRCLAGHEYRDFAIIDDRRIVFKGPGNSLWLNELRARCPDLRRADTLAFRSHT
                     MSERICELDSFVASDWMDSLSRRSPGMRCTLGSFQPISEPQLQALRDLSKR"
     gene            complement(6723..7595)
                     /locus_tag="AC16QF_11950"
     CDS             complement(6723..7595)
                     /locus_tag="AC16QF_11950"
                     /inference="COORDINATES: protein motif:HMM:NF012899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR6449316.1"
                     /translation="MSVSDSQPLLIVALGGNALSPPGRGGEGYADERRIVAQTARTLR
                     DLAGLGRRLLIVHGNGPQVGRLLRTDPEPGNLDIHVAQTQGELGYLIARELAPEPVVG
                     VLTRVVVSEPLGAPVKPIGPILAERPQKAASVPAGSGWRLVVPSPKPARVVEIKAVAQ
                     LLKSSHVVAGGGGGVPLDEEGNPLNCVVDKDWVASLFAVGLHAQSLVFATDVSGVHPD
                     PERQGERIDRLSVADAEQLLASDAVAPGSMAPKLASAVEFVSSSGRSAGICDLESLAA
                     FAGGDESAGTVITP"
     gene            complement(7582..8913)
                     /locus_tag="AC16QF_11955"
     CDS             complement(7582..8913)
                     /locus_tag="AC16QF_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449317.1"
                     /translation="MSQERRRVVICGAAGRDFHNFNVAFRDDPQVQVVAFTAAQIPGI
                     DRRRYPAELAGSRYPEGIPIIPEAELESVCRQQSVSEVIFAYSDVSYAAVMEVASRVL
                     ATGAGFELLGPDATMLKAGVPVIAVCAVRTGCGKSQTARFVSSELVRGGRRVAVMRHP
                     MPYGDIAAQRVQRFASMADMDAAGCTLEEREEYEPHVAAGGVVYAGADYGAVLEQAEQ
                     GVDVLVWDGGNNDFSFIRPDFHIVVVDALRPEQLDTHYPGAAVLRTADLVVVNKVRSA
                     SADQLDQVSAGLDRIVPGVPRVLAASPVVLDEPERLTGARVLVVEDGPTITHGGMPTG
                     AGYRAVHELPVARIIDPRTCAAGSIAALYDRYPHIGPILPAMGYGPAQLDELRETVAR
                     SGADYVVAGTPIDLARALETDIPVLRARYSYVDAGSPGLMDHVKDFLDERI"
     gene            complement(8915..9709)
                     /locus_tag="AC16QF_11960"
     CDS             complement(8915..9709)
                     /locus_tag="AC16QF_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449318.1"
                     /translation="MTERVTRVLLTLECDRLDADAMKAVVRLAGDGELEMLGLFVEDE
                     DLLRAARLPGLTEVSVNTGEVKTVSVEQIQEQVAGQARRAREVFEASARGLKVKHSFR
                     IARGRAVDAVAEAALTSDIVVVSRALRASGLRSRRGVHFESLVKKHGNILFVNEPWAS
                     GASVVVLCESTPTDCERPLSVARRIADAENIELVLAVPPGAQSVERSPADRVVHLDAW
                     SEDAVAALCDQEDARLLVLPPTEKLDWRALLMNIVDRVPCSLLRLD"
     gene            complement(9702..>10279)
                     /locus_tag="AC16QF_11965"
     CDS             complement(9702..>10279)
                     /locus_tag="AC16QF_11965"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HIR6449319.1"
                     /translation="SSFLGSRYAREMPLSLHASLVFEQSYGGVEGDSASIAEVCALIS
                     AISERPIRQCLAITGSMDQHGVAQAIGGVNEKIEGFFDICVEQGLSGDQGVIIPVSNC
                     DHLMLREDVVDAVREGKFHVYSMRTVDDAMQRLFAADGETLADVETIDAAVRDRLTQW
                     HRIWRRAAAEAGAAEGAEGGERGMGKDAGDD"
BASE COUNT         1861 a         3566 c         3155 g         1697 t
ORIGIN      
        1 tggccaggcc gggcagcttc agctcgcggt ccatctcgtc gatgagttct cgcgtggtct
       61 tggtgggatc gggccactcg cggcgcggct tgagccgaat cgtggtttcg aacatcgcca
      121 ggggcgctgg atccgtggcc gtctccgcac gaccggcttt cccgaagacc tgcccgacct
      181 cgggaaactc gcgaaggata cggtcggtct gctggagcag ttccctggcc ttggagatgg
      241 agactccggg aaacgtggtg ggcatgtaga gaatgtcccc ctcatccagc ggcggcatga
      301 attccgaccc gagacgcgag aacggcaccc acgtgagcgc cagcagcaag ccggccatca
      361 ccaccgtcgc ccagcgccag cgcatggcca aacccagcag cggcgtgtgg agacggtgca
      421 gccatcggtt gatggggttg gattcctccg gccgaatacg ccctctgacc agatagccca
      481 tgagcacggg taccagcgta atggccagga tcgctgcgcc cgccatcgcg taggtcgccg
      541 tgaaagccag cgggctgaat aaccgccctt cctgggcctg catcgagaat accggcagaa
      601 aggccacggt gatcacgagc agcgagaaga acagggccgg accaacctcg cgagccgctg
      661 cgcccacggc ttgccatcgc tcggccgatg agagctcggc acccttgcgc tcggcgtcgc
      721 gggccatgtg tttgtgcgcg ttctcaacca tgacgatcgc cccatccacc atggcaccga
      781 tggtgatcgc gatccctcca agggacatga tgttggcgtt caggccatgc cagcgcatca
      841 gcaggaaagc aatgagcacg cccagcggga ggatcaagac ggccaccatc gccgaccttg
      901 catgggccag aaagacgatc accaccaagc tgacgatgac gatctcgatg agcagcgacc
      961 ggctgagggt atccaccgcc cgatcgataa gatccccgcg gtcatagacc gtcacgatct
     1021 ccacaccttc cgggagaccg gccctgagct cgtccagccg ttggcgcacg ccttcgatgg
     1081 tggcgcgcgc attttcccca taacgcatca ccacgatgcc gcccaccact tcgccgtctc
     1141 catccagatc caccagccca cgcctgagct cgggccctat ctgaatccgc gccacatccc
     1201 caaggcggat gggcgtaccg cccggatccg cggccaccgg tatcgacccg aggtcctcca
     1261 gagatcgaat gtagcccagg ccccgcacca tgtactcggt ctcggagatt tccaatagcc
     1321 gcccgcccac atcgttgttc gaacgttgaa tggcctgctt gattttcgac agcgggatac
     1381 caagggccat gagcgcgttg gggtccacct ccacctggta ctgcttgaca aacccgccaa
     1441 cggaagcaac ctccgcaacg ccaggcacgg tctgcagcgg atagcgcaga taccagtcct
     1501 gaagagacct gagctgcgcc agatcgtggc ggccggaccg atccaccagg gcgtactcgt
     1561 atatccagcc cacgcccgtc gcatcagggc caagcgcggg gttgacgccg gccggcaggc
     1621 ggccgctgac gtagttgagc gactccagaa ctctggatct cgcccagtac atgtcggtgc
     1681 cgtcttcgaa tatcacgtag acgaaagaca agccgaagaa agagtatccg cggaccacgc
     1741 gggccttggg aacggcgagc atcgctgtgg tgagcgggta ggtcacctga tcttccacga
     1801 cctggggcgc ctgtccggga tagtcggtaa acacgatcac ctgcacgtcg gacaggtcgg
     1861 gaatggcatc cacgggcacg gtgaggtagg cccatgtacc cgcaccggcg aggatgagcg
     1921 ctccgagcag aactattaac cgctcgccga tcgccccatc gatgatgcgc gcgatcacgg
     1981 cgacgctcca cggtcatgcc cgccgtggtg atggtcgtga tcgccgactc cgtcgggcga
     2041 cgacgcttca tcacccgggc gcgccatcat cttcgcagca gcctcgcgga gcttggattc
     2101 cgaatcgatc agaaactggc cggacgtgac cacttcctcg ccctcggcaa gcccttcgag
     2161 aacactgact cgaccctcgg actccacccc gagctcgacc acccgcggct caaagcgacc
     2221 gggcgcacgc acgacgaaca cctgattggt cagacccgag cggacgaccg cctccgcggg
     2281 aatgaccagc gcgttgggct ccgtcgacgt acggatgagc acgtcggcga acatctccgg
     2341 tctcagaagc cggtccgggt tgtcgaaaac caggcgcgcc ttgaccgttc gggtcttcgc
     2401 ttccgcatag gggtatatgt agtccagcaa ccccccgaat gtctctcccg gcagcgctcc
     2461 cagcgtcatc tcgaccgcat cgcccacccg aacccagggc agatcgtgct cgaacacgtc
     2521 ggcgtaaacc cacacccggg agagatcggc aatcacgtag agctcggtca tgggcgtcac
     2581 gtgctgaccc tggcgggccc cgatcttcag gaccacgcct tcagcaggcg agtgaatgtg
     2641 cagcgacttc tgcgggacgc gggatttttc cagctcgacg atctgatgct ccggcacgtc
     2701 cagcagctcg agccgggcac gggaactcgc gacgagatcg gccgcgccgc ggctgatgtc
     2761 gggaatgggg ctgtcgttga gcgactcgta gttcctgagc gcaagcacgt attcctgctg
     2821 gctggccacc aggcgcggcg agtacacgct caacagcatg gtgtcctcac tgacctgagc
     2881 accggtggtg tccacgtaca tttcctcgac ccaaccttcg accttggggt gcagcctggc
     2941 aatactccgc tcgtcgaagt cgatacggcc taacgcgcgc acggtgtggc tgagcgcggc
     3001 gcgctgagcg cgagccgttc tgacgccgat gttctgcacg acgatgggat cgatggaaac
     3061 cgtccccgcc gggccggcag cctcctcgtc cgcgtaaacc gggatgtagt ccatacccat
     3121 ggagtctttc gccgggaccg cagaggtgac ggacgggttc atggggttac gatagaagat
     3181 gggttctcgc gccctcccct ccggctccag cggtgccgtt tcgctcggtt tgcccgccga
     3241 cagctccgcg aagaagtagc ccgcgcccac cccgccggcg agcgccacaa tcaccagcag
     3301 cagaaatttc ttacccacct cgcacctcct cgcccaccgc ggcgctcaac cgcgcgacgg
     3361 actgattcgc cagagtgacc gctttccagt acagcgtttc gtagttgtag agcgtgacct
     3421 ggctgcgcac gagattcaga aaatccacct cgccgacctg ataaccggcc agcatcgact
     3481 gcaccgtctg gcgcgcctga ggaatgattc ccgagtcgag cagcaccagc tgctcgcggg
     3541 cgcgccggta gtcggcgacc gcttgggaaa tctccgcccg catggcattg acctcgtcct
     3601 gcagcgcgta gcggtgtcga tccatctcgc tgcggcgctg gctgactgcc tgagcgcgct
     3661 tccggctctg gtagatgggc agattgacgc tcaccctgag ggtcaacaga tccgttctgt
     3721 cgccaccccc aggcatcggg ttctcgccgg atcgagcgcc gtacgccgcg cccacggtga
     3781 aatcgggata gtaatctttg cgcgcaagct cgagcctgga ttccgccgcg tcgatctcgc
     3841 tgcgcaggcg cttgagcagc ggacgcgatt ccgccccgcg ctggtacagc tcctgctgcg
     3901 cgacgagatc cggaaagacg ccgacgggcg tcctgggcag ctcgatgggt tggttggccg
     3961 gctggtccag cagacggttc agcctggcag cttccacgtc gcgggcgcca ttgagctgga
     4021 tcgcctggtc gagcagcttt gaaagttcca gctgcgccag caggacatcc tgctgcagcc
     4081 caccgcccac ctcgtagcgg gtcttggcaa tctggacaaa ctggcgcagc agttcctggt
     4141 tccgctgaac gatctcctgg gcgcgatcga gatagtgcag ggtccaccag ttgaaggaca
     4201 cgtccctgat cagccgcagc cgggtctcgg ctacgctgct gcgagcggcc tccgcctcgt
     4261 ggctggaagc cgcttcgcgc agcgcccgtt tgcctggaaa cggtatggtc tggctgatcc
     4321 ccacctgcag ctgggtcatg ggctcctggc ccacgtggaa agtgtccgtg gggaagttca
     4381 cggcaccgaa gctcagcacc ggatcaggga gagttcccac ctgggagggt atcgcagcca
     4441 tcgcttcata gcgcgactga atacccgcca ggttcgggtt cgtgcgcacc gctctctcca
     4501 gggccagctc cagcgtcaaa cccgtccgga gcagatcgcc gccgtcctcg gagccttggg
     4561 caacacccgc aaccgccagg gacgcgacca gccacgtcct catcattcgt cgtttcatcc
     4621 gccacaccct cgttcagttc actgatgacc gagaaatgag cggcaccatg ccgactccca
     4681 gaagaccgca acgggccatg ggaactgggg cgccagcagc gccgctcgat cagcagtcga
     4741 ggattggagg acggaataag gacgtgggag gcgcgcggac gtgaccgctc agccagtcgg
     4801 tgacgaaccg tttcggaccg gatcgcagtg gcgcttccag agcactcccg gcgagaaagg
     4861 acgtcgatcc cgaggcggca cactcctgga gacactcgtc gcagcaatca cagtccgcgg
     4921 ccccgtcttc gacggacacc tccccatggt cgccatgatg ctcatcgtaa ccggcgtgct
     4981 cgggaccatg acccacatga tgcccggctg cacgcgcagg tgcttcgccg gccccctggc
     5041 aggaaccagc caacgccgtg agcggcgtcg tcagcataag cgctaccagc agatgcgcaa
     5101 caagggcatt cacgaggctc atgagcgaag gctatcgcga gatcccccgc tccacaacgc
     5161 ctacaccgtg ccgttgccaa ctggttcctc tgattgagca agaggatggt caaaacacct
     5221 gcccgaggcc ccgtcgcaac cggtcgtggt gtaacatctc cggccacccg atctgcctgg
     5281 ccatgcccgc catgaccacc gaacagcttc tgcgctttgc gacctacgca tccgtggcta
     5341 cagccacggt gctcatcgcg gcgaaactga ccgcctggtt cgccaccgat tcggtgagcg
     5401 tcctggcctc actggtcgat tcggtcatgg acgtgggagc atcgctgatc aatctgtttg
     5461 ccgtgcgcta ctcgctcatg cccgccgacc aggaacaccg cttcgggcat ggcaaagccg
     5521 aggcgctggc cggtctgggt caggcgacct tcatcgccgg ctccgccata ttcctgatac
     5581 tgcacgcagt agaccgacta atggatcccg tcacgatcga gcacgtcgcg gcaggctcgg
     5641 cggtgatggg attcgccatc gtcatgacgc tggtgctgct gacgattcag cggcacgtaa
     5701 tccgccgcac cggttcgact gccatacgcg cggatgcgct gcactacgca acggacctgg
     5761 ccaccaacgc ggccaccctg ctcgcgctgt tgctggcggc ctacggtttc tcagggttgg
     5821 atccgctgtt cggcctggcc atcgcagcct acatccttta ctccgccagc cgcatcggtc
     5881 gagacgccgt cagcctgctg atggaccggg agctgcccca agccgagcgc gaggaaatca
     5941 agcgagtcgt ggacggcacg cctggggtgc tgggcgttca cgggctgcgc acctggcagt
     6001 ccggacagcg caaggtcatc cagatgcacc tggagctgga cccggacctc ccgctcagag
     6061 aggctcaccg catcgccgtg gaggtggagc gacgcctgct cgaacacgag ccggaggctg
     6121 acatcaccat ccatcaggat ccggcgggac agccatccca cgttgctcac aaccctggcg
     6181 acgcgttcag ttgactcatc caatggggtc agcggggcga tttgtcagtc gaccgcgttt
     6241 cgggctaacg ttcgtcgcca tgagcacccg gattctaatg ctctccatgc tgctgctcgc
     6301 cggctgcgcg tcggcgcccg aggccgagca ggcccagcag cagcgggagg aagacattgc
     6361 cacgattctg aacgaacccc tcgacccgtc ggagtacggt gaggccaaac gctgcctggc
     6421 gggccacgag taccgcgact tcgccatcat cgacgaccgc cgcatcgtgt tcaaaggtcc
     6481 tggaaacagc ctgtggctga acgagcttcg ggctcgctgc cccgacttgc ggcgggccga
     6541 tacgctggcc tttcgatctc ataccatgag cgagcggatc tgcgagctcg acagcttcgt
     6601 agcgtcggac tggatggaca gcctgtcgcg ccgctcgccc gggatgcgct gcacgctcgg
     6661 ttctttccag cccatcagcg agcctcagct gcaggccctt cgcgatctgt ccaagcgctg
     6721 actcagggtg tgatcaccgt gccggcggat tcgtctccgc ccgcaaacgc ggcgaggctc
     6781 tccagatcac agattcccgc ggaccggccc gatgagctga cgaactccac ggcgctggcg
     6841 agcttcggag ccatggaccc cggcgcgacg gcgtccgaag ccagcagctg ctccgcgtcg
     6901 gcaacgctca gccgatcgat ccgttcaccc tggcgttccg ggtccgggtg aacgccggac
     6961 acgtcggtgg caaagacgag actctgggcg tgcaggccaa cggcaaacag gctggcaacc
     7021 caatctttgt ccaccacgca gttcagcggg ttaccctctt cgtccaaggg caccccgccg
     7081 ccgccgccgg ccacgacgtg gctcgatttc agcagctgcg ctacggcctt gatctcgacg
     7141 acgcgagccg gtttcggcga gggcacgact agccgccacc ccgaccccgc aggcaccgat
     7201 gccgctttct gagggcgttc ggcgagaatc gggccaatgg gcttgaccgg cgcgccgagc
     7261 ggctcggaga cgaccacccg tgtcagcacg cctaccacag gctccggcgc aagctccctg
     7321 gcgatcagat atcccagctc gccctgggtc tgggccacgt ggatgtcgag gttgccgggc
     7381 tcggggtcgg tccgcagcag gcgcccgacc tgaggcccgt tcccatgaac gatcagcagt
     7441 cggcgaccca ggccggccag atcgcgcagc gtccgggcgg tctgcgccac gatccggcgc
     7501 tcatcggcgt atccttcgcc gccccgcccg ggcggcgaca gggcattgcc gcccagcgcg
     7561 acgatcagca gcggctggga atcagatacg ctcatcgaga aagtctttga cgtggtccat
     7621 caatccgggc gatccggcat cgacgtacga ataccgggcc cgcaacacgg ggatgtccgt
     7681 ctccagcgcc cgggcgagat cgatgggcgt tcccgccacg acgtagtccg cgcctgatcg
     7741 tgcgaccgtc tcgcgcagct cgtctagctg tgcgggtccg taccccatgg ccgggagaat
     7801 ggggcctatg tgcggatatc tgtcatagag cgcggcgatc gaacccgcag cgcacgttcg
     7861 cggatcgatg atccgggcga ccggaagttc atgcaccgcc cgatagccgg ccccggtggg
     7921 catgccaccg tgggtgatgg tcggaccgtc ctccacgacg agcacccggg caccggtcag
     7981 cctttccggc tcgtccagga ccaccggaga cgccgcgagc acccgcggca cacccggcac
     8041 gattcggtcc agccccgcgc tcacctgatc cagctgatcc gcgctcgcgc tgcggacctt
     8101 gttcaccacc accagatcgg ccgttcgcag caccgcggca ccggggtagt gggtgtccag
     8161 ctgctcagga cgcagcgcgt cgacaacgac gatgtgaaaa tcggggcgaa tgaacgaaaa
     8221 gtcgttgttg cccccgtccc agacgagcac gtcaacgccc tgttcggcct gctccagaac
     8281 ggctccgtag tctgcccccg catacacgac cccaccggca gccacgtggg gctcgtactc
     8341 ttcccgctct tccagggtgc agccggccgc gtccatgtcc gccatggagg caaaccgctg
     8401 cacccgttgg gccgcgatgt cgccgtaggg catgggatga cgcatcacgg cgacgcgcct
     8461 gccgcccctg acgagctcac tcgacacgaa gcgggccgtc tgagatttac cgcaaccggt
     8521 gcgcacggca cacacggcga tgacgggaac gcccgccttg agcatcgttg cgtctggccc
     8581 gagaagttcg aacccggcac ccgtggccag aacccgcgag gccacttcca tgaccgcggc
     8641 gtagctcacg tcgctgtagg caaagatcac ctcgctcacc gattgctgcc ggcagacgga
     8701 ctcgagctca gcctccggaa tgatgggtat gccctcggga taccgcgaac ccgcgagctc
     8761 ggccgggtag cggcgcctgt cgatgcccgg aatctgggca gccgtgaagg ccacgacctg
     8821 cacctgggga tcatcgcgaa acgcgacgtt gaagttgtga aaatcacgcc cggcggctcc
     8881 gcagatgacc acccggcggc gctcctggct cacttcagtc gagcctcaac agcgagcagg
     8941 gcacccggtc cacgatattc atgagcaatg cccgccaatc gagtttctcg gtgggcggga
     9001 ggaccagcag cctggcatcc tcctggtcgc agagcgcggc gacggcatcc tccgaccagg
     9061 cgtccagatg aacgacccga tcggccggtg atcgctccac gctctgggcg ccgggcggga
     9121 cggcaagcac gagttctatg ttctcggcat ccgcaatccg acgggctacg ctcagcggcc
     9181 gctcgcaatc ggtcggcgtg gactcgcaga gcacgaccac gcttgcgccg gacgcccaag
     9241 gctcgttgac gaacaggatg ttgccatgct ttttaaccag cgattcgaaa tgaacgccgc
     9301 gccgcgagcg cagtccagac gcccgcagtg cccggctcac gacgacgatg tcggaggtca
     9361 atgctgcctc cgccacggcg tccacggccc gcccgcgggc gatgcggaac gagtgcttga
     9421 ccttgagccc ccgggcagaa gcctcgaaga cctcccgggc ccgccgggcc tggccggcca
     9481 cctgttcctg tatctgctcg acggacacgg tctttacctc gcccgtgttc accgacacct
     9541 cggtgagccc cggcagccgg gcagcgcgca ggaggtcctc gtcctccacg aacagcccga
     9601 gcatctcgag ctcgccgtct ccggccagcc gcaccaccgc cttcatcgcg tcggcgtcca
     9661 gacggtcgca ttccagggtg agcagcaccc tggtaacccg ctcagtcatc gccggcatcc
     9721 ttccccatgc ccctctcccc gccttccgcg ccttccgccg cgccggcttc cgcagcggcg
     9781 cgacgccaga tccgatgcca ttgcgtgagg cggtcccgca cggcggcatc gatggtttcg
     9841 acgtcggcga gcgtctcgcc gtccgcggca aacagcctct gcatggcatc gtctacagtg
     9901 cgcatggagt aaacgtggaa cttcccttca cgaacggcat ccaccacgtc ctcccgcagc
     9961 atcaggtggt cacagttcga cacggggatg atgaccccct ggtcaccgct caagccctgc
    10021 tcgacgcaga tgtcgaaaaa tccctcgatc ttctcgttga cgccgccgat cgcctgggcg
    10081 acgccatgct ggtccatgga ccccgtgatc gccaggcact gccttatcgg gcgctcgctg
    10141 atcgcggaga tcagcgcgca gacctcggca atggacgcgc tgtcgccttc caccccgccg
    10201 taagactgct cgaacaccag gctggcgtgc agagacagcg gcatctcacg cgcgtaccgg
    10261 gagccgagaa aggacgaga
//
LOCUS       DBNHLM010000255        12400 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_635661, whole genome shotgun sequence.
ACCESSION   DBNHLM010000255 DBNHLM010000000
VERSION     DBNHLM010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12400)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12400)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12400
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_635661"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="AC16QF_11970"
     CDS             <1..644
                     /locus_tag="AC16QF_11970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6449320.1"
                     /translation="YLAGVTENLRLGTGVSLIPQRNPIYTANEFATLDYLTGGRVDLG
                     VGVGWCKEEVLACGYEWETRGERCDEILELMIKLWTEPVTTHHGKHFRVDDCRMDPKP
                     VQQPHVPLIVGGYSPAAMRRAARYGAGWIGFGTNPEQTAMALKGIDAALEAAGRSRDG
                     FEIVVMPAEANADTVRAFEDIGVDRLVPLLVASGPDAVARRIDELAELKALLA"
     gene            complement(669..1196)
                     /locus_tag="AC16QF_11975"
     CDS             complement(669..1196)
                     /locus_tag="AC16QF_11975"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6449321.1"
                     /translation="MVRETGQVPGLEEVADRLAILDVLAKHSRGVDRADAEVLKSAYW
                     PDAQVAYGGFNGLAHEFCDYLPGAIKRYARTQHSISNTAIEFSGDEARVETYVTAYHY
                     LEVDGDQGTEMTYLGRYMDRMQKRGDVWKIIHRQVVMDWNQNVDTTAVWEGPTFSGLA
                     RGARAPDDPVNALLG"
     gene            1275..2423
                     /locus_tag="AC16QF_11980"
     CDS             1275..2423
                     /locus_tag="AC16QF_11980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012336.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6449322.1"
                     /translation="MSTDVAIIGIGIHPFGRTEGVSGLDQGVFAARQALKDVRLDWTD
                     MQFAYGGSAAAGSADTMVNKLGLTGLQFINVANGCATGGSALQSAFMAIQSGMFDIGM
                     AVGFDKHPRGAFTVDPEKRGLGKWYGDIGMALTPQFFAMKIQRYMHDYGITEDALTKV
                     AVKNYKNGALNPNAWRRNAFSYEEVAQSPMVCDPLRKYMFCSPAEGGVAMILCRADKA
                     AEYTSKPVFLDAVSVKTRNFGSFEVYTQGQALDRADSPTVMASKAAFEMAGIGPEDID
                     VAQLQDTESGAEIMHMAENGFCADGDQEKWLAEGRTEIDGRLPVNTDGGCLANGEPVG
                     ASGLRQVYEVALQLRGEAGERQVPNTPRTGYTHVYGAPGLSGVTILSR"
     gene            complement(2437..3981)
                     /locus_tag="AC16QF_11985"
     CDS             complement(2437..3981)
                     /locus_tag="AC16QF_11985"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6449323.1"
                     /translation="MAEKVETVRTTDPAYHRLLDTETRPVPESYRRDSPIEPGPTFVP
                     VERYISREFFDLEVEKIWKRVWQMACHEDDLPDVGDYVPYDIAGMSFLVVRTGEDEFK
                     AYYNACLHRGRKLRENRGKAATDLRCPFHGWTWNLDGSLKQIPCQWDYPGLKMSEQSL
                     PEVKVGRWGRYIFLNPDPDCGPLEDFLGDLSSHFELLPYEKRYKEAHVARIIRCNWKV
                     ASEAFMESYHVIATHPQIILGGVHDTDTKYDVFGNYSWAIRCGALESSGLPDWGELPQ
                     DGRRRVRHPLNGFVYEATDDGLVQVTNPEGKTGLFTANADHVSGELGDVNPHLCNWVG
                     GRQLEPSEFEKAMASRAAKQSGETVKHPRVLFADIQREALAKVIPSIADKIADIEFSS
                     VFFTVFPNFHPWGSFNRINYRFRPNGRNVDECIMECIYLAPIPENGEYTPCREIHWLG
                     PDDDWTEAPELGMLAKVFNQDVRNLPYVQEGLHATAKEHLQFADYNETKPRHLHMLMD
                     EWIAKP"
     gene            4145..5542
                     /locus_tag="AC16QF_11990"
     CDS             4145..5542
                     /locus_tag="AC16QF_11990"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6449324.1"
                     /translation="MDYDLTKPADPTLAPGDARCPGPSTRDIILKEQDGAPAALTAES
                     WSFLGDEDIPFSRYTSQPFYDREIETIWRKTWQWACREEHIPNPGDWTVYDVGPYSVV
                     VIRDEGGAVKAYVNSCPHRGMQFCDAGSSGRGKQFLRCPFHGMSWHLDGSLREIPCRW
                     DFPHIEDENFGLTEVPTGTWAGFVFINLDPDCEPLESYLEVLPEHFKNWKLEDRFVAL
                     HTRKILPGNWKMCMEAFLEAYHVLATHPEGIHSAGDANADYDIFGRHVTRFVHTIGYP
                     SPHVASEVDERKLFAALGHNPDDLPDGMSARQRHADIMREQLGREWDVDLSGLSNSEM
                     LDSIEYFVFPNACFFPGINIRLVYRFRPIDVDHTMHEILLLQPVPEHGERPKPAEPID
                     LTIDESYEKANEVSLGGFALAMVLDQDTENFHRQRAGIKAAQRAGVRTAAVLANYQES
                     RIRRLHMTVDEYMAG"
     gene            5565..7160
                     /locus_tag="AC16QF_11995"
     CDS             5565..7160
                     /locus_tag="AC16QF_11995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019750117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR6449325.1"
                     /translation="MIIGAGTAGCVLANRLSADKDADVLLLEAGGKDDYFWIDIPVGY
                     LYTINNPRTDWCYQVEPEPGLNGRRINYARGKVLGGCSSINAMIYMRGQRGDYDHWAQ
                     LGNRGWSWDDVLPVFKRSEDYQHGAGEFHGAGGELRVEERRVDWEILDAWRDAAQECG
                     IPKIEEFNRGDNFGNAYFQVNQRRGVRWSASKAFLRPAMARPNLEVLTNAHVQRLEIE
                     TTDAGLRASGVTVQMGDDAPTTITARREVVLAAGAIGSPQILQLSGVGPGDLLHEHGI
                     RVIHDLPGVGENLHDHLQVRSVYKVHGTVTLNQRANSLIGKALMGLEYFTRKTGPLTM
                     PPSQLGAFAKSDPSVASADIEWHVQPLSLDAFGEPLHRFNAITPSVCNLRPTSRGHLR
                     IKSPDPADYPAITLNYLATEHDRQVILAGMRLTRRIMAAKALERFQPEEWKPGPSLTT
                     DEELLTAAGDLGTTIFHPVGSCRMGSDPMAVVGDRLAVHGVEGLRVIDASVMPQITSG
                     NTNAPTVMIAEKGAEFMRADAAS"
     gene            complement(7170..7676)
                     /locus_tag="AC16QF_12000"
     CDS             complement(7170..7676)
                     /locus_tag="AC16QF_12000"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR6449326.1"
                     /translation="MSESLDKALTVARDRVVRFHYVIRDEAGSELESTREGEPLAVLQ
                     GRGSVLRGVEEALEGRNAGERLEVTLPPEQAFGARREDWTQRVPKKYLPDAKRLKPGD
                     AAVLRTREGPRPVTVVKVGSKVVDVDLNHPRAGMTLTFDLEIVEVREATPEEIAHRHV
                     HGPGGHRH"
     gene            complement(7699..8949)
                     /locus_tag="AC16QF_12005"
     CDS             complement(7699..8949)
                     /locus_tag="AC16QF_12005"
                     /inference="COORDINATES: protein motif:HMM:NF015557.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR6449327.1"
                     /translation="MPIELLLIVCLVAIFLVPIFFDVNVGIVAFITAFLVGALAMDLS
                     ARQVLAGFPDNMFVMLVGITLLLAIAHQNGTVDWIVAKLVRAARGRLVLLPWVLFVTA
                     LVTASLGPVAAPVLFVIGVGFVSRYGLNPLMVAAMIIHGTQSGAYSPIAPYGVVIGEL
                     AATAGIGYSPVAVYAGVVTFHVLLAAFVFLMLGGRALAGVRVEVAIGETAESERLDRQ
                     RTLTLIGFACLVLGLLVYQINLGFLAMSIALVLLLTGPREDRQAAVGNIAWPVVLVIA
                     GVLTYVGLLQHAGATDWLALQAKAMGSPLLMGLVLCFIVAVVTGVASTIGTIGILVPL
                     SAPFVLAGDLEATGLLAAMAVSAAVSDISPYSTWGAMFLATAGQVLNRQEILQAQLKY
                     TIVMVALMPILAWLVFVVPGWQVG"
     gene            complement(8992..9786)
                     /locus_tag="AC16QF_12010"
     CDS             complement(8992..9786)
                     /locus_tag="AC16QF_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6449328.1"
                     /translation="MASITEFAVRDHVAYITLNRPEALNSLNPELRWTLSRHFDEVEG
                     NDDIWLAVVTGAGERAFCAGADLKHRARERDASAEQRAEWARMMEETTPLNQRWYLAK
                     PVIARVNGFALGGGLELALSCDIIVAAEHAELGLPEPRRGLIAGGVGVHRLPRQIGLK
                     AAMGYLLTGRHMSAQRAYELGLVNQVVPGAGLDAAVDEWVRDILRCAPLAVRATKEAA
                     MRGLDLPLPQAFYTEYEWERRRRGSDDALEGPRAFAEKRPPEWRGR"
     gene            9934..10608
                     /locus_tag="AC16QF_12015"
     CDS             9934..10608
                     /locus_tag="AC16QF_12015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HIR6449329.1"
                     /translation="MANYFEGIFGRSPFKPVQEHMDLCHQAAALTEELLARAGSQSWP
                     EVEEIREQIVALERRADDRKRDIRLHLPRGFFLPVARADLLDLLARQDKIANKAKEIA
                     GLIVGRRMSFPPAIQESALELTRTSISACQRALDVVNELDELIATGFRGVEVQRVEEM
                     IDALDESESRNDDAQVRLRAELHGLERELDAVDVIFLYRIIDRIGDLADNAQSVGHRL
                     EMMLAR"
     gene            10662..11915
                     /locus_tag="AC16QF_12020"
     CDS             10662..11915
                     /locus_tag="AC16QF_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR6449330.1"
                     /translation="MDPTTLLLLLALLFGLYMAWGIGANDVANAMATSVGARAITVKQ
                     AILIAAVFEFLGAFLAGGDVTSTIRKGIIDSSALVGDPDVLILGMLAALLAAAVWLTV
                     ASRFGWPVSTTHSIVGAIVGFAIATIGMEAVHFDKVGSIAASWVVSPLLAGTLSFLLV
                     RSVQWLILDRPNPELYARRYVPAYIFLAALNISLVTLLKGLKHIGLDLTAFEAIGYSV
                     AFALVIMAGGAVMVRRIHTATATEMEDYHYANVERTFAVLMIVTACAMAFAHGSNDVA
                     NAIGPVAAIISTVQSGEVFQKASVPPYVLLLGAAGIVIGLATYGYRVMATVGRKITEL
                     TPSRGFAAELAAASTVVLASGTGLPISTTHTLVGAVLGVGLARGISALNLGVIRTIFM
                     SWVITLPVGAVLAVLFYYMLRAALL"
     gene            complement(11949..>12400)
                     /locus_tag="AC16QF_12025"
     CDS             complement(11949..>12400)
                     /locus_tag="AC16QF_12025"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6449331.1"
                     /translation="RFNLTSTFLGTKRALKYMLPAGYGTIVNISSIEGKRGTPVMSAY
                     CACKHGINGFTKAVAAEVGRSGVTVNAICPGLIITDAITTGGPVMAETMGMTFDEMVE
                     TVFKANTLTGELNTVEQIGDMTVFLASTAGRGITGQQISVDCGMSPY"
BASE COUNT         2192 a         4211 c         3961 g         2036 t
ORIGIN      
        1 gctacctggc cggcgtgacg gaaaatctca ggctaggcac cggcgtctcg ctgattccac
       61 agcgcaaccc gatctacacc gccaacgagt tcgcaacgct ggattacctc acgggcggca
      121 gggtcgatct cggcgttggc gtaggctggt gcaaggagga agtgctggcc tgcggctacg
      181 agtgggagac ccggggcgag cgctgcgacg agatcctgga gctgatgatc aagctgtgga
      241 ccgagcccgt caccacccac cacggcaagc acttccgcgt ggacgactgc cgcatggacc
      301 cgaagcccgt gcagcagccg cacgtcccgc tcatcgtggg cggctacagc cccgccgcca
      361 tgcgccgagc cgcgcgctac ggcgccggct ggatcggctt cggcaccaac cctgagcaga
      421 cggccatggc actcaaaggc atcgatgccg cgctggaagc cgccgggcgc tcccgggacg
      481 gcttcgagat cgtcgtcatg ccggccgagg ccaatgcgga caccgtgcgc gcgttcgagg
      541 acatcggcgt cgaccggctg gtcccgctgc tggtggcgag cggtcccgac gccgtggcgc
      601 ggcgcatcga cgagctggcg gagctcaagg cgctgctggc ctgacccggc ggcgagccgg
      661 ccgggatgtc agcctaggag cgcgttgacc gggtcgtccg gggcacgggc gccccgcgcc
      721 agccccgaga acgtcggccc ttcccagacg gccgtcgtgt ccacgttctg gttccagtcc
      781 atgaccacct ggcggtggat gattttccag acgtcaccgc gcttctgcat gcggtccatg
      841 tagcggccga gataggtcat ctcggtgccc tggtcgccgt cgacctccag gtagtggtag
      901 gcggtcacgt aggtttccac ccgcgcttcg tcgcccgaga actcgatggc cgtgttgctg
      961 atgctgtgct gagtacgcgc gtagcgcttg atagcgcctg gcaggtagtc gcagaactcg
     1021 tgggcaagac cgttgaaccc cccgtacgcc acctgggcat ccggccagta ggccgacttc
     1081 aggacctcag catccgcgcg atccacgccc cggctgtgct tggcaagaac gtccaggatg
     1141 gcgagccgat cggccacctc ctccaaaccg ggcacctgcc ccgtctctct gaccatgatg
     1201 cgatccctcc ctgacgaccg agcccaagac aatactgtag aatctgctca tctgaaaccg
     1261 ggaggtgagc aggcatgtct acagacgtag cgatcatcgg tatcggcatc cacccttttg
     1321 gacggacgga gggcgtctcc ggcctggacc agggcgtgtt cgccgcgcgg caggcgctca
     1381 aggacgtgag gctcgattgg accgacatgc agttcgccta cggcggaagc gccgccgccg
     1441 gctccgcaga cacgatggtc aacaagctcg ggctcaccgg gctgcagttc atcaacgtcg
     1501 ccaacggctg cgccaccggc ggcagcgctc tgcaatccgc cttcatggcc atacagtccg
     1561 gcatgttcga catcggcatg gccgtgggtt tcgacaagca cccgcgcggc gctttcaccg
     1621 tggatccgga aaagcgcggt ctgggcaagt ggtacgggga catcggcatg gcgctcacgc
     1681 cgcagttctt cgccatgaag attcagcgct acatgcacga ctacggcatc accgaggacg
     1741 cgctgaccaa ggtggcggtg aagaactaca aaaacggcgc tctgaacccc aacgcctggc
     1801 gacgcaacgc cttcagctac gaggaggtgg cccagtcgcc catggtctgc gacccgctgc
     1861 gcaagtacat gttctgctca ccggccgagg gtggcgtggc catgatcctg tgccgcgcgg
     1921 acaaggcggc cgagtacacc agcaagccgg tctttctcga cgcggtcagc gtgaagactc
     1981 gcaacttcgg ctccttcgag gtttacaccc agggccaggc gctcgaccgg gccgattcgc
     2041 ccacggtgat ggcgtccaag gccgccttcg agatggccgg catcggcccg gaggacatag
     2101 acgtcgccca gctgcaggac accgaatccg gcgccgagat catgcacatg gcggaaaacg
     2161 gcttctgcgc cgacggcgat caggagaagt ggctggccga agggcggacg gagatcgacg
     2221 gcaggctgcc ggtcaacaca gacggcggct gcctggccaa cggcgagccc gtgggcgcgt
     2281 caggcctgcg acaggtctac gaggtggcgc tgcagctcag aggagaggcc ggtgagcggc
     2341 aggtgcccaa cacaccccgc acgggttaca cccacgtgta cggcgcaccg ggcttgagcg
     2401 gcgtcaccat cctgtcacgc tgaagctacc gccgggtcag ggcttcgcga tccactcgtc
     2461 catcagcatg tgcaggtggc ggggcttggt ctcgttgtag tccgcgaact gcaggtgctc
     2521 cttggcggtg gcgtgcagac cttcctgcac gtaggggagg ttgcgcacgt cctggttgaa
     2581 caccttcgcc agcattccca gctccggcgc ctccgtccag tcgtcgtcgg gccccagcca
     2641 gtgaatctcc ctgcacggcg tgtactcgcc gttctcggga atcggtgcca ggtagatgca
     2701 ctccatgatg cactcgtcca cgttccggcc gttcggacgg aaacggtagt tgatccggtt
     2761 gaacgacccc caggggtgaa agttgggaaa gaccgtgaag aagacggacg agaactcgat
     2821 gtccgcgatc ttgtccgcga tcgagggaat caccttggcc agcgcctcgc gctggatgtc
     2881 ggcgaagagc acccgcggat gcttgacggt ctcgcccgac tgcttggccg cccgggaggc
     2941 catggccttt tcgaactcgg acggctccag ctggcggccg ccgacccagt tgcacaggtg
     3001 cgggttgaca tcccccagct cgccggagac gtggtccgcg ttcgccgtga acagccccgt
     3061 cttgccttca gggttggtca cctggaccag cccgtcgtcc gtggcttcat aaacgaagcc
     3121 gttcaacggg tgacgcaccc ggcgccgacc gtcctggggc agctcgcccc aatcgggcag
     3181 gccgctgctc tccagggcgc cgcagcggat ggcccacgag tagttgccga acacgtcgta
     3241 cttggtgtcc gtgtcgtgga ccccgcccag gatgatctgc gggtgggtgg cgatcacgtg
     3301 gtaagactcc atgaacgcct cggaagccac cttccagttg cagcggatga tcctggctac
     3361 gtgggcctct ttgtagcgct tctcgtaggg cagcagctcg aagtggctcg agagatcgcc
     3421 cagaaagtct tccagcggcc cgcagtccgg atcggggttg aggaagatgt agcggcccca
     3481 gcgacccacc ttgacctcgg gcaggctctg ctccgacatt ttcaggccgg gatagtccca
     3541 ctggcacggg atctgtttga gcgaaccatc caggttccag gtccagccgt gaaacgggca
     3601 acgcagatcg gtggcggcct tgccccgatt ttcacgcagc ttgcggcccc ggtgcaggca
     3661 ggcgttgtag taggccttga actcgtcctc cccggtccgg accaccagga acgacatgcc
     3721 ggcgatgtcg tagggcacgt agtcgccaac gtcgggcagg tcgtcttcat ggcaggccat
     3781 ctgccagacg cgcttccaga tcttctctac ctcgaggtcg aaaaactccc tggagatata
     3841 gcgctccacc ggcacgaagg tgggtccggg ctctatggga gaatcgcggc ggtaggactc
     3901 cggaaccgga cgggtctcgg tatccagcag ccggtgatag gcggggtccg tggtgcggac
     3961 ggtttcaact ttttctgcca tgacgcgttc tcccctgaac tgccggaata ctaccccgat
     4021 ctggtcacaa ggaatacggc tcgcgcggag cccgccaggt aacccgatga cgtcaccgcg
     4081 ctccggttgg ctcgctggcc gaaccatgta tcatcagtgc agcacctacc cggggaatac
     4141 caagatggac tacgacctca ccaaaccagc cgacccgacg ttggctccgg gagacgcgcg
     4201 ctgtcccgga ccgagcaccc gcgacatcat cttgaaggag caggacgggg cgcccgccgc
     4261 gctcacggct gaaagctggt cgttcctcgg agacgaggac attccgttct cgcgctacac
     4321 gtctcagccg ttctacgacc gggaaatcga aaccatctgg cgcaagacct ggcagtgggc
     4381 ttgccgcgag gagcacatcc ccaacccggg tgactggacc gtttacgacg taggaccata
     4441 ctcggtggtc gtcatccgcg acgagggcgg cgcggtcaaa gcctacgtca attcctgccc
     4501 gcaccggggc atgcagttct gcgacgcggg ctcatccggg cgcggcaagc agttcctgcg
     4561 ctgtcccttt cacggcatgt cgtggcacct ggacggctcg ctcagggaga ttccctgccg
     4621 ctgggacttc ccccacatcg aagacgagaa cttcggcctg accgaggtgc ccacaggcac
     4681 gtgggccggc ttcgtcttca tcaacctcga tcccgactgc gagccgctgg aaagctacct
     4741 ggaagttctt cccgagcact tcaagaactg gaagctcgag gaccgcttcg tggccctgca
     4801 cacccgcaag atcctgccgg gcaactggaa gatgtgcatg gaggcgttcc tggaggccta
     4861 ccacgtgctc gccactcatc ccgaaggcat tcacagcgcc ggcgacgcca acgccgacta
     4921 cgacatcttc ggccgtcacg tcacccgctt cgtgcacacc atcggctacc cgtcccccca
     4981 cgtcgcctcg gaggtggacg agcgcaagct gttcgcggcc ctcggccaca acccggacga
     5041 cctgcccgat ggcatgagcg cccggcagcg ccacgcggac atcatgcgtg agcagcttgg
     5101 gcgcgagtgg gacgtggatc tgagcgggct ctccaacagc gagatgctgg attccatcga
     5161 gtacttcgtc ttccccaacg cctgcttctt ccccggcatc aacatccgcc tggtgtaccg
     5221 cttccggccc atcgacgtgg accacaccat gcacgagatc ctgctgctgc agcccgtgcc
     5281 ggagcacggc gagcggccca agccggccga gcccatcgac ctgaccatcg acgagtcata
     5341 cgagaaggcc aacgaggtct ccctcggcgg gtttgcgctg gccatggtct tggaccagga
     5401 cacggagaac tttcaccgtc agcgcgcggg catcaaggcc gcacagaggg cgggtgtgcg
     5461 aacggccgcc gtcctggcca attaccagga gtcccggatc cgccggctgc acatgacggt
     5521 ggacgagtac atggcgggtt agtcaggttc gcgagttcga ctacatcatc atcggagcgg
     5581 gcaccgccgg ctgcgtgctg gcgaaccgtc tgagcgctga caaagatgcc gacgtcctgc
     5641 tgctggaagc cggcggcaag gacgactact tctggatcga catcccggtc ggctacctgt
     5701 acaccatcaa caacccgcgc acggactggt gctatcaggt ggagcccgag cccggcctca
     5761 acggccgccg catcaactac gcccgcggca aggtcctcgg cggctgctcg tccatcaacg
     5821 ccatgatcta catgcgcggt cagcgcggcg actacgatca ctgggctcag ctcggcaatc
     5881 ggggctggtc gtgggacgac gtgctgccgg tgttcaagcg ctccgaggac taccagcacg
     5941 gcgcgggcga gtttcacggc gcgggaggcg agctgcgcgt cgaggagcgg cgcgtcgact
     6001 gggagattct ggatgcctgg cgggatgctg cccaggagtg cggcatcccc aagatcgagg
     6061 agttcaaccg cggcgacaac ttcggcaatg cctactttca ggtgaaccag cggcgcgggg
     6121 tgcgctggag cgcaagcaaa gccttcctgc gcccggccat ggcgcgtccc aatctcgagg
     6181 tgctcacgaa cgcccacgtt cagcgcctgg aaatcgagac caccgacgcc ggccttcgcg
     6241 cctccggcgt caccgtgcag atgggcgacg acgcgcccac caccatcacc gcgcgccggg
     6301 aagtggtgct tgccgcaggc gccatcggct ccccccagat tctgcagctc tccggtgttg
     6361 gccccggcga tctgctgcac gagcacggca tccgggtgat ccacgacctg ccgggcgtgg
     6421 gggagaacct ccacgaccac ctgcaggtgc gctccgtcta caaggtgcac ggcacggtga
     6481 cgttgaacca gcgggcgaac tccctcatcg gcaaggcgct gatggggctg gagtacttca
     6541 ccaggaaaac cggaccactc accatgccgc cctctcagct gggcgcgttc gcgaagtccg
     6601 atccgtcggt cgcctccgcg gacatcgagt ggcacgttca gccgctgtcg ctggacgcct
     6661 tcggggagcc gctgcaccgc ttcaacgcga tcaccccgtc ggtgtgcaac ttaaggccca
     6721 ccagccgcgg acatctgcga atcaagagcc cggaccccgc cgattatccg gcgatcaccc
     6781 tcaactacct cgcaaccgag cacgaccgtc aggtcatcct cgccgggatg cggctcaccc
     6841 gccggatcat ggcggcaaag gcactggagc ggtttcaacc ggaggagtgg aagcccggcc
     6901 ccagcctgac taccgacgag gagctgctca ccgccgccgg cgatctcggc accaccatct
     6961 ttcacccggt gggcagctgc agaatgggca gcgaccccat ggctgtggtc ggcgaccggc
     7021 tggccgtgca cggcgttgaa gggctgcggg tgatcgacgc ttcggtaatg ccgcagatca
     7081 cctccggcaa caccaacgcg cccacggtga tgatcgctga gaagggcgcg gagttcatgc
     7141 gggccgacgc ggcctcctga gacaccgcct cagtgccgat ggcccccggg gccgtgcacg
     7201 tggcggtggg cgatctcctc gggcgtggct tcgcgcacct ccacgatctc gagatcgaac
     7261 gtcagggtca tccccgcgcg gggatggttg agatccacgt ccacgacttt gctccccacc
     7321 ttcaccacgg tgaccggacg cggcccctcc cgggtccgca gcacggccgc atcgccaggt
     7381 ttgagccgtt tggcatcggg cagatatttc ttgggaacgc gctgggtcca gtcttcccgc
     7441 cgggcgccga aagcctgctc gggaggaagg gttacttcca ggcgctcgcc ggcgtttcgg
     7501 ccctccagcg cttcctcgac gccccgcagc acgctgccgc gcccctgcag cacggccaga
     7561 ggctcgcctt cgcgcgtgga ctcgagctcc gagcccgcct cgtcgcgaat cacgtagtga
     7621 aagcgcacca ccctatcccg cgccacggtg agggccttgt ccaaagactc cgacatcact
     7681 ttctccccga tagacttctc atcccacctg ccacccgggc accacgaaca ccagccacgc
     7741 caggatgggc atcagcgcga ccatgacgat ggtgtacttg agctgggcct gcaagatctc
     7801 ctggcggttc agcacctgac cagccgtagc caggaacatc gcgccccagg tggaatacgg
     7861 agagatgtcc gaaaccgcgg cggagacggc catagccgcc agcagccccg tggcttcgag
     7921 gtcgccggca aggacgaagg gtgcggatag cggcaccaga atgccgatgg tgccgatggt
     7981 ggacgctacc cccgtaacca ccgccacgat gaagcacagc acgaggccca tgagcagcgg
     8041 cgagcccatt gccttggcct gaagggccag ccagtcggtg gcgccggcat gctgcagcag
     8101 acccacgtag gtcagcacac cggcgatcac cagcaccacg ggccaggcaa tgttgcccac
     8161 cgccgcctga cggtcttccc ggggaccggt gagaagcaga accagcgcga tgctcatggc
     8221 gagaaagccc aggttgatct ggtacaccag gagccccagc accagacagg caaaaccgat
     8281 caaggtcagc gtgcgctggc ggtcgagccg ctctgactcg gcggtttcac caatggcaac
     8341 ctctacccgc acgccggcca gggcccgccc gcccagcata aggaacacga aggcggccag
     8401 cagcacgtga aaggtcacca cgcctgcgta gacggccacg ggcgagtagc cgatcccagc
     8461 ggtggcagcg agctcgccga tcaccacccc gtagggcgcg atgggagagt aagcgccgga
     8521 ctgggtgccg tggatgatca tggcggcaac catcagcggg ttgagcccgt atcgggagac
     8581 gaaccccacg ccgatcacga acagcaccgg cgcggccacg ggcccgagag atgcggtgac
     8641 cagcgcggtg acgaagagca cccacggcag cagcaccagt cgtccccggg cggctcggac
     8701 gagctttgcg acgatccagt ccacggtgcc gttctgatgg gcgatggcga gcagcagcgt
     8761 gatgcccacc agcatgacga acatgttgtc gggaaaaccg gccagcacct gtctcgcgct
     8821 caggtccatg gccagcgccc cgaccaggaa ggcggtaatg aaggccacga tgcccacgtt
     8881 gacgtcgaag aagatcggca ccaggaagat ggcgacgagg cagacgatga gcaggagctc
     8941 tatgggcatg acgtcaacgc tgccagatcg gccgcacgat ggctcgcagg ctcagcgccc
     9001 ccgccactcg ggcggacgtt tctcggcaaa ggccctgggc ccttccagag catcgtcgct
     9061 gccccgccgg cgccgttccc actcgtactc cgtataaaag gcctggggca gcggcaggtc
     9121 gaggcctcgc atggcggcct ccttggtggc ccgaacggcc agcggcgcgc agcgcagaat
     9181 atctctcacc cactcgtcca ccgccgcgtc gagccccgcc ccaggcacca cctggtttac
     9241 caggcccagc tcgtaagccc gctgggccga catgtgccgg cccgtgagga ggtaacccat
     9301 ggccgctttc aggccgatct gccgcggcag ccgatgcacg cccacgccgc cggcgatcag
     9361 tccccgtcgg ggctccggca ggccgagctc ggcgtgctcg gccgcaacga tgatgtcgca
     9421 ggacagcgcc agctccagcc cgccgcccag cgcgaagccg ttgacccgcg cgatgacggg
     9481 cttggccagg taccagcgct gattgagggg ggtcgtttct tccatcatgc gcgcccattc
     9541 ggcgcgctgc tcggcgctgg cgtcgcgctc gcgggcgcgg tgcttgaggt cggcgccggc
     9601 gcagaacgcc cgctcgccgg ctccggtcac caccgccagc cagatgtcgt cgttaccctc
     9661 cacctcgtcg aaatgtctgg agagcgtcca gcgcagctca gggttcaggg agttgagcgc
     9721 ctcggggcgg ttcaaggtaa tgtacgccac gtgatcgcgc accgcgaact cggtaatcga
     9781 tgccatcgtc cccttccccg aactcaccca gacgagtaac cggtcaatct aacgtggaaa
     9841 tcagagcccg gcaatcggca agaagaccga atgtccactt ccctttgccc agccacgtgg
     9901 gttagttttc gccgcgggaa gggagacata tccgtggcga actacttcga gggcatcttc
     9961 ggccgctcgc ctttcaagcc cgtacaggaa cacatggatc tgtgccatca ggccgcggcg
    10021 ctcaccgaag agctgctggc cagggcggga tcccagtcct ggcccgaggt ggaggagata
    10081 cgcgagcaga tcgtcgccct ggaacgccgg gccgacgacc gcaagcggga catccgcctg
    10141 cacctgcccc gaggcttctt cctgccggtt gcgcgcgccg acctgctgga cctgctggcg
    10201 cgccaggaca agatcgccaa caaggccaag gagattgccg ggctcatcgt ggggcggcgc
    10261 atgtcttttc cgccggccat tcaggaatcg gctctggagc ttacccgaac gtccatctcg
    10321 gcctgccaac gtgcgctgga cgtggtcaac gagctcgacg agctcatcgc gacgggcttc
    10381 agaggcgtag aggtgcagcg ggtggaagag atgatcgacg ccctcgacga aagtgaatcc
    10441 cgcaacgatg acgcccaggt ccgcctccga gcggaactgc atggtctgga gcgggaactc
    10501 gatgccgtgg acgtgatatt tctgtatcgc atcatcgatc gcatcggtga tctggctgac
    10561 aacgcccaga gcgtcggcca caggctggag atgatgctgg cgcgttgagg gcgcaagctt
    10621 cctggcgctg agccgacaga aaaaccggga aagggatggc gatggacccg acgacactgc
    10681 tactgctgct cgcgctgctg ttcggcctct acatggcctg gggcatcggc gccaacgacg
    10741 tcgccaacgc catggccacc tccgtcggcg cccgggccat taccgtgaag caggcgattc
    10801 tgatcgccgc ggtgttcgag tttctgggcg cttttctcgc cggcggagac gtcacgtcca
    10861 ccatccgtaa aggcatcatc gactcgagtg ctctggtggg cgatccggat gttctgatac
    10921 tgggaatgct cgccgcgctg ctcgccgcag cggtgtggct cacggtggcc tcgcggttcg
    10981 gctggccggt ctccaccacc cactccatcg tcggcgccat cgtcggcttt gccatagcca
    11041 ccatcgggat ggaggccgtt cacttcgaca aggtcggctc catcgcggcc agctgggtcg
    11101 tctccccgct gctggcgggc acgctctcgt ttctgctggt ccggtccgtg cagtggctga
    11161 tcctggaccg cccgaacccc gagctgtacg cccggcgcta cgttcccgcc tacatcttcc
    11221 tcgccgcgct gaacatctcg ctggtgacgc tgctcaaagg gctcaaacac atcggcctgg
    11281 atctgaccgc tttcgaggcc atcggctatt ccgtggcctt tgccctggtc atcatggccg
    11341 gcggcgccgt gatggtgcga cgcatccaca ccgcaacggc cacggagatg gaggactacc
    11401 actacgccaa cgtggagcgc acgtttgccg tgctgatgat cgtcaccgcc tgcgccatgg
    11461 ccttcgccca cggctccaac gacgtggcca acgccatcgg ccccgtggcc gccatcatct
    11521 ccaccgtgca gtccggcgag gtcttccaga aagccagcgt gccgccctac gtgctgctgc
    11581 tcggcgccgc gggcatcgtg atcggcctgg ccacctacgg ttaccgcgtc atggccacgg
    11641 tagggcgcaa gatcacggaa ctgaccccca gccgcggctt cgcagcggag ctcgccgcgg
    11701 catccaccgt ggtgctggcc agcggaaccg ggctgcccat ctcaaccacc cacaccctgg
    11761 tgggcgccgt cctgggcgtg gggctggccc gtggcatcag cgctctgaac ctgggcgtca
    11821 tccggaccat cttcatgtcc tgggtgatca cgcttcccgt gggcgccgtg ctggccgtcc
    11881 ttttctacta catgcttaga gcggcgctgc tctaaccggg ccatcgccgc cctggggccg
    11941 gggccgtctc agtagggcga catcccgcag tccaccgaga tctgctgacc ggtgatgccg
    12001 cgccccgcgg tggaggccag aaacaccgtc atgtcaccaa tctgctccac ggtgttcagc
    12061 tcgccggtca gggtgttcgc cttgaacacc gtctccacca tctcatcgaa cgtcatcccc
    12121 atggtctccg ccatgaccgg gccgccggtg gtgatggcgt cggtgatgat caggcccggg
    12181 cagatggcgt tgaccgtgac ccctgagcgg cccacttcgg ccgccaccgc cttggtgaac
    12241 ccgttgatgc cgtgcttgca cgcgcagtag gcggacatga ccggcgtgcc gcgctttcct
    12301 tcgatggagg agatgttgac gatggtgccg tacccggcgg gcagcatgta cttcagggcc
    12361 cgcttggttc ccagaaacgt cgaggtgagg ttgaagcgca
//
LOCUS       DBNHLM010000256         1736 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1533256, whole genome shotgun sequence.
ACCESSION   DBNHLM010000256 DBNHLM010000000
VERSION     DBNHLM010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1533256"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            397..1602
                     /locus_tag="AC16QF_12030"
     CDS             397..1602
                     /locus_tag="AC16QF_12030"
                     /inference="COORDINATES: protein motif:HMM:NF013068.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HIR6449332.1"
                     /translation="MKESITTLPARESTVDLLRESLGSRIREVIEELLQAELDEVLGA
                     ARHERTGDRTGYRNGSYERQLVTEAGPTALTVPRARIENEDSKTREHQSEILPKYERR
                     SRQVNEAIVGCYLAGANSRRIRKALQPLLGSSQLSKSAISRVVIKLKARFDEWMQRDL
                     SEEGSLVLYLDGMHLPVRLVRRVVRVPVQAVVGVRESGEKLLLSLRIAPSESTASWKG
                     VVEDLAGRGLHAPKLVVVDGNKGLLRAIHDTWSTADVQRCTKHKLENLLGAAPKHCHD
                     ELKRDYRDIVYADGLELAQKAYDRFCRKWSKLCKEVVVSLQEAGHSLLTFYRYPKSQW
                     KSLRTTNQIERLNGEFRRRTKTQGSFSNEKAALVLLWALVAFGQIQMRKIDGWQDMPE
                     IVAMLNQAA"
BASE COUNT          350 a          509 c          591 g          286 t
ORIGIN      
        1 ccacgaggat gaccgcgttc tcgagtggag ccgcgtcgtc gccgttgacc agcgtgcctc
       61 ccaccaccgc gatcttgccc tgcaatgtcg ccgccggcgc cgtgaggagc agggcgatca
      121 gcatcaccga gcagtggaag gagcgggacc tcgcgcgagt tctcatgagc ttctctccca
      181 tcgtggcgcg gacatcggct ttctttcggg ggcgcccgcg cccgtgacgg atgatcgatc
      241 gggcggagcg gagctttgcc atcgatcgac ctcgagtcag cgggtcaggc tgacacccgg
      301 gtagcgtccc gactgttgtg gaaaaaggag ttggcgtagt ctctgggtga aaactcagac
      361 gcccggaagt gggctaaccc aaaggactac gccaacatga aggaatctat cacaaccctg
      421 cctgccaggg aatccacggt tgatctgctg cgagaatccc ttggctcgag gatccgcgaa
      481 gtcatcgagg agttgctcca ggccgagctc gacgaagttc tcggtgcggc gcggcacgaa
      541 cggaccggcg atcggacggg ctatcgcaac gggagctacg agcgtcagct ggtgaccgag
      601 gcagggccga cggcgctcac ggtaccccga gcccggatcg agaacgagga cagcaagacc
      661 cgcgagcacc agagcgagat cctgcccaag tatgagcgcc gcagccgtca ggtgaacgag
      721 gcaatcgtag gctgctacct ggccggcgca aacagccggc gaatccgcaa ggcgctgcag
      781 ccgttgttgg gtagctccca gctctccaag agcgcgatct cgcgggtggt gatcaagctc
      841 aaggcccgat tcgacgaatg gatgcagcga gatctctctg aggaggggtc gctggtgctc
      901 tatctcgacg gcatgcacct gcccgtgcgg ctggtccggc gcgtggtccg tgtgccggtc
      961 caggcggtgg tcggggtgcg tgagagcggc gagaagctgc tgctttcgct tcggatcgcg
     1021 ccgagcgagt cgacggcatc ctggaagggg gtggtagagg atctcgccgg tcgagggctt
     1081 cacgcgccga agctcgtggt ggtcgacggc aacaagggcc ttctgagagc gattcacgat
     1141 acctggtcca cggccgacgt ccaacgttgc acgaagcaca agctggagaa cctgctcggt
     1201 gcggccccca agcactgcca cgacgagctg aagcgggact accgcgacat cgtgtacgcg
     1261 gacggcctcg agctcgcgca gaaggcctac gatcgtttct gtcgcaagtg gtcgaagctg
     1321 tgcaaggagg tcgtcgtgag cctgcaggag gcggggcact ccttgctgac gttctaccgc
     1381 tacccgaagt cgcagtggaa gagcctgcgg acgacgaacc agatcgagag gctgaacggg
     1441 gagtttcgtc gccggacgaa gacacaaggc tcgttcagca acgagaaggc cgcgctggtt
     1501 ctgctctggg cgctggtggc ctttggccag atccagatga ggaagatcga cggctggcag
     1561 gacatgcccg agatcgtcgc gatgttgaac caagcagcgt agacagctac gctgcgaatc
     1621 cgtacccgga ggctacgtca gacattttcc acaagaagcg gggcacgacc gcagcagcgc
     1681 ttgccgggat agaaacacgg atgccaatgg ttgtagaatt gagtcaagca tgccac
//
LOCUS       DBNHLM010000257         6014 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_336072, whole genome shotgun sequence.
ACCESSION   DBNHLM010000257 DBNHLM010000000
VERSION     DBNHLM010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_336072"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="AC16QF_12035"
     CDS             complement(<1..156)
                     /locus_tag="AC16QF_12035"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR6449333.1"
                     /translation="MEKSDFYANARSDLPGPYHGVVVVEATTTWAGPMAGCILADFGA
                     RVIKVEHP"
     gene            complement(180..1151)
                     /locus_tag="AC16QF_12040"
     CDS             complement(180..1151)
                     /locus_tag="AC16QF_12040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007979206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase family protein"
                     /protein_id="HIR6449334.1"
                     /translation="MVGVYRIHEAGGPEVLSWESEELPDPTGELVRIRHTAIGLNYID
                     TYHRSGLYPLPYPTRLGVEAAGFVEAAGDAVTDLKEGDRVAYAGGMGAYAEANNVPAS
                     RLVRIPDNLADETVAACLLKGLTACYLLTRTYPLAANDTMLLHAAAGGTGLIMCQWAR
                     HIGATVIGTAGSQEKAELAEHNGADHTILYREEDVVARVREITGGRGVNVAYDSVGLD
                     TYQVTLKSLAPLGMFVSFGNASGKVPPVDPQDLVSNGSLFFTRPSLAHYTLRPEDLRA
                     MADQLLGLVANRTVEIHINQRYALKDLVQAHRDLESRSTSGSTIIIP"
     gene            1371..1964
                     /locus_tag="AC16QF_12045"
     CDS             1371..1964
                     /locus_tag="AC16QF_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6449335.1"
                     /translation="MELILVRHGLPVREELETGRADPPLSETGRAQAERVALWLADAP
                     VDAVYSSTMQRARQTAQPYAAAIGLEVVEREGIAEFDRHESSYVPLEELKAEDPEAWR
                     AFVAGGYNEQVDIHQFHAEVVNSLEQLIARHSGQRIAVFCHGGVINVWTAHVIGITPR
                     LFFEPAYTSIHRFLCARSGERNLVSLNETGHLRAPIT"
     gene            2199..2972
                     /locus_tag="AC16QF_12050"
     CDS             2199..2972
                     /locus_tag="AC16QF_12050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6449336.1"
                     /translation="MSAVVNVRSKKVPTLYEADESKYAGPHRWQIRAWARVARRLWRY
                     RAYTRWARQAFSRIEVSGAEHLDSVEGPCVFIANHQSHLDTILVHAVLPEKIRSKIYF
                     GAAQDRWFVKGKRKLVLKPWYQSLALGNFPILRGGGTRALAYASWLLDRGQHVFLFPE
                     GTRATSTEVGEFKHGAAILALEAGVPVVPVYIAGAQKIRPKGTREVARGVGGIEILEP
                     VRFSAGSDVAAATRLLQDRLGEVHARHRGGDRERLPSAA"
     gene            complement(2977..3171)
                     /locus_tag="AC16QF_12055"
     CDS             complement(2977..3171)
                     /locus_tag="AC16QF_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017738733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HIR6449337.1"
                     /translation="MTHYEVRSVSLSCPYCGETIEILIDLSLSEQEYIEDCSVCCRPI
                     LLDVSVTDDEPLVTARSENE"
     gene            complement(3168..4148)
                     /locus_tag="AC16QF_12060"
     CDS             complement(3168..4148)
                     /locus_tag="AC16QF_12060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6449338.1"
                     /translation="MLVRRLITIPAFIAATVAVTAALPVLAIAAAICSVVPATKGALP
                     TLGFIAGYLWCETIGIVTAFAIWIRHREADRFLQANFRLQCWWANALKVIAERLFRLT
                     FDVEGEECLSGPAAIMLPRHASIADTIIPMVYYAIPRQIRLRYVLKRELLADPCLDIV
                     GHRLPNYFVDRHGQDSERARRGVAALLADIGDDEGALIYPEGTRFSEAKRSQLLERYA
                     ASEDMLAQLRRWTELLPPRLGGTLALLDANPGRDLIFCAHTGFEGSSHFATLINGSWI
                     GAHIRISFWRVPGQAVPAETDAQREFLFDQWDRMQAEVLRLQAAPGGTAP"
     gene            complement(4138..5406)
                     /locus_tag="AC16QF_12065"
     CDS             complement(4138..5406)
                     /locus_tag="AC16QF_12065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR6449339.1"
                     /translation="MMLGVSSSILVAMLEIGFIGQLGTESVAAITFTFPVVMILSSIA
                     LGVGIGTSSVIARSVGAGQLEDGRRLGTHSLLLVVAMISALSFIGWLAIDPVFLALGA
                     PAHMLPLIHSYLDIYYPSVVFFATTMAAGNIMRASGSANVPGLVMTLGAVVNLALDPI
                     FIFGWFGFPRLELAGAAVAGAISRVGTAAILLYFVARGGLLLTTGWKPRFLNSCHRIL
                     EVGLPAMATQLIGPVSSAVITRLLAGHGEIVVAGFGVATRVEAVAVMLLFALSGSIGP
                     FVGQNWGAGRSERVREGLRFTYLFCLAWGLAAAILMAFFGSTVASWVDSEPGVIGTAA
                     FYLAVVPWSYGLWGVLMMSSASFNALGKPIPSTILSFTRMFIMYIPLAVLLEGQFGYT
                     GIFVATAATNGVMGILAFLWFRSAYFADAR"
     gene            complement(5582..>6014)
                     /locus_tag="AC16QF_12070"
     CDS             complement(5582..>6014)
                     /locus_tag="AC16QF_12070"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6449340.1"
                     /translation="FTASSVMSESFGRYPFNNANERALITLAVEEDFEIHAPRLLVVH
                     VLFNLIKNALYYVQRAGKGSITISCARRCITVHDTGSGISRVARSQVFDRFYTTTSTG
                     QGAGIGLSFCKMVMESIGGSITFESAEDEHTTFILEFPAAT"
BASE COUNT         1159 a         1855 c         1946 g         1054 t
ORIGIN      
        1 ggggtgctcc accttgatca cccgcgcccc gaagtcggcc aggatgcatc ctgccatcgg
       61 tcctgcccag gtggtggtcg cctccacgac gacgaccccg tgatacggtc cggggagatc
      121 gctgcgcgcg ttggcgtaga agtctgactt ttccatgacc ttggtttcct gaatcggggc
      181 tacggaatga tgatggttga accggacgtg ctgcgggatt ccagatcgcg atgggcctgg
      241 accaggtcct tgagcgcata gcgttgattg atgtgaatct cgaccgttcg gtttgcgacc
      301 agtcccagca gctgatcggc catggctcgc aggtcctccg gccggagcgt gtaatgcgcc
      361 agggaaggtc gggtaaagaa aagcgagccg ttgctgacga gatcctgggg gtctaccggc
      421 ggaaccttgc cggaagcgtt gccgaagctc acgaacatgc cgagcggagc cagacttttc
      481 agagtcacct ggtaggtgtc caggccaacg gagtcgtagg cgacgttgac gcctcgtccg
      541 ccggtgattt ccctcaccct ggctaccacg tcctcctccc gataaagaat cgtgtggtcg
      601 gcaccgttgt gctcggccag ctcggccttt tcctggctac ccgctgtccc gatgaccgtc
      661 gctccgatat ggcgcgccca ctggcacatg atcaggccgg taccgccggc cgcggcatgg
      721 agcagcatcg tgtcgttggc ggccaggggg taggtgcgcg tcagcaggta gcaggctgtg
      781 agtcccttga gcaggcaggc ggcgaccgtc tcgtcggcga ggttgtccgg gatacggacc
      841 agtctgctgg caggcacgtt gtttgcctcc gcataggcgc ccatcccgcc cgcatatgcc
      901 actcgatccc cttcctttag atccgtcacg gcgtcccctg cggcctcgac gaagcctgcc
      961 gcttccacgc cgagtcgggt ggggtaggga agtgggtaga gaccgctgcg atggtaggtg
     1021 tcgatgtaat tcagaccgat cgcagtgtgt ctgatgcgca ccagctcgcc ggtcgggtcg
     1081 ggaagctctt ccgactccca gctgagaacc tcgggaccac ctgcttcgtg gatgcgatag
     1141 acgccgacca tgctccctcc ccaatcgagt catgaaccgc aagtctcggc catggcgcct
     1201 ggaggtgcaa tcgccctgca atgacccgac gagccctgcc gtgccgctgg tcggtgagcc
     1261 tcggctatac ttcgacgcgg gtcgctcggc ccgccccgcg gccgatccgg cacctccagc
     1321 ttccgggcgg tccgccgtcg caggccgaag acaccacaga ggacctcatc atggagttga
     1381 tactcgttcg tcacggatta cccgttcgcg aggaactgga gaccggacgt gcggacccgc
     1441 cgctttcaga gaccggcagg gcgcaggctg agcgcgtcgc gctgtggctg gccgatgcgc
     1501 ccgtcgatgc cgtctactcg agcaccatgc aaagggcgcg gcagacggca cagccctacg
     1561 cggccgctat cgggctggag gtcgtcgagc gggagggaat cgccgagttc gaccgccacg
     1621 agtccagcta cgtgcccctc gaggagctca aagctgagga tccggaagcg tggcgggcgt
     1681 tcgtcgccgg cggctataac gagcaggtgg acattcacca gttccacgcc gaggtggtga
     1741 actccctgga acagctcatc gcccgccact ccggtcagcg aattgccgtg ttctgccacg
     1801 gcggcgtgat caatgtgtgg acggcgcacg tcatcgggat cacgcctcgt ctgttcttcg
     1861 agccggctta cacgagtatc caccggttcc tgtgcgctcg ttccggtgag cgcaatctgg
     1921 tcagcctcaa cgagaccggg cacctgcgcg cgccgattac ctgacctccc atccgaccgg
     1981 ccgcgtctcg ctttgcatcg aagtggtgcg cggccgaacc cagtggtccc aagttggtgc
     2041 gccggttacc cgccgatgcc gggcgaaggc cgaaatacgg tgccttcact ttggcctgca
     2101 aattgcaatc tccatggggt aacggtcctg tcatggacgc gtcctggagt gcaacgctcc
     2161 ttcgggcgag gtcatcaaca aggcattgga ggtcagtgat gtcggcggta gtaaacgtca
     2221 ggagcaaaaa ggtccccacc ctgtacgagg cggacgagtc gaagtacgcc gggccgcacc
     2281 ggtggcagat tcgggcgtgg gcacgggtgg ctcggcggct ctggcgctac cgggcataca
     2341 cccgttgggc acggcaggcg ttcagccgca tcgaagtctc gggtgcggag cacctcgact
     2401 cggttgaggg gccttgcgtc ttcattgcca atcaccagag ccatctcgac accatactgg
     2461 tacacgccgt gctgccggag aagatccgtt cgaaaatcta cttcggcgca gctcaggata
     2521 gatggttcgt caaaggtaag aggaagctcg tactcaagcc ctggtatcag tcgctggctc
     2581 tcggaaactt tcccatcctc cgtggcggcg gcaccagagc gctggcgtac gccagctggc
     2641 tgctcgaccg tgggcagcac gtgttcctct ttcccgaggg cacccgtgca acctcgacgg
     2701 aggtcgggga gttcaagcac ggcgcggcca tcctcgcgct cgaagcaggc gttcccgtcg
     2761 tgcccgtcta catcgcggga gcgcagaaaa tcaggcccaa aggcacgcgg gaggttgccc
     2821 gaggggtggg tggaatcgag attctggaac cggtgaggtt ctctgcgggg agcgacgtag
     2881 ccgccgcgac ccggcttctg caggaccgtc tgggtgaggt tcatgcccgg cacaggggtg
     2941 gtgatcggga gaggctgcct agcgccgcct agcgcgtcac tcgttctcgc tgcgcgcggt
     3001 caccaggggc tcgtcgtcgg tcacggagac gtccagcagg atgggccggc agcaaacgga
     3061 gcagtcttcg atgtattcct gctccgacag gctgaggtcg atcagaatct cgatggtctc
     3121 gccgcaatac ggacagctca gggagacgga gcgaacctcg tagtgggtca cggggccgtt
     3181 cctcccggag cggcctgcag acgaagcacc tcagcctgca tcctgtccca ttggtcgaac
     3241 aggaattcgc gctgggcatc ggtctccgcc ggcacggcct ggcctggcac gcgccagaag
     3301 gagatgcgga tgtgggcgcc gatccaggat ccattgatga gcgtggcgaa gtggctcgac
     3361 ccctcgaagc ccgtgtgcgc gcagaaaatc agatcccgcc ccggattggc atcgagcagc
     3421 gcaagggttc cgcccagacg cgggggcagc agttcggtcc agcgtctaag ctgggcgagc
     3481 atgtcctccg aagccgcgta gcgctcgagt aactgcgatc gttttgcttc ggagaaccgg
     3541 gtgccttccg ggtagatcag cgcgccctca tcatcaccga tgtcggcgag caacgcggcc
     3601 acacctcgtc gggcccgctc gctgtcctgg ccgtgccgat cgacgaagta gttgggtagc
     3661 cggtggccga ctatgtccag gcaggggtca gccagcagct cccgtttcag cacgtacctg
     3721 agcctgattt gccggggaat tgcgtagtag accatgggga tgatcgtgtc ggcgatgctg
     3781 gcgtgtcgcg gcagcatgat tgccgccgga cccgaaagac actcttcgcc ctcgacatcg
     3841 aaggtcagcc tgaagagcct ctccgcgatg accttcagcg cgttggccca ccagcattgg
     3901 agcctgaagt tcgcctggag gaatcggtct gcctcccgat gccggatcca gatcgcgaat
     3961 gccgtgacga tcccgatggt ctcgcaccac aggtagccgg cgatgaatcc aagcgtcggc
     4021 agcgcgcctt tggtagctgg aacgacgctg caaatggccg ccgctatcgc cagcacggga
     4081 agcgccgcgg taacggccac cgtggccgca atgaatgccg gaatcgtgat caagcgccta
     4141 acgagcatct gcaaaatacg cgcttcggaa ccagaggaac gccagtatgc ccatgacgcc
     4201 gttggttgct gcggtcgcga cgaatattcc cgtatagccg aactgccctt ccagcagcac
     4261 ggccagcgga atgtacatga tgaacatccg cgtaaacgag aggatggtgg agggtatcgg
     4321 cttgcccagc gcgttgaacg acgccgatga catcatcaga acgccccaga gaccgtagct
     4381 ccaggggacc accgcaagat aaaacgccgc ggttccgatc acaccgggct cagagtctac
     4441 ccatgatgcg acggtgctgc cgaagaacgc catgagaatc gcggctgcca gcccccaggc
     4501 caggcagaag aggtaggtga agcgcaggcc ttcccggaca cgctcggacc ggcctgcgcc
     4561 ccagttctgg ccaacgaatg gaccaatgct gccggacaag gcgaagagca acatgaccgc
     4621 cacggcctcg acccgggtcg caactccgaa gcccgcaacc acgatctccc catgaccggc
     4681 caggagtcga gtgatcacgg ccgaggaaac cggtccgatg agctgggtgg ccatggcggg
     4741 aaggccgacc tccagaatcc gatggcacga gttgagaaat ctgggcttcc acccggttgt
     4801 cagcaacagg ccgcccctgg caacgaaata gagcagaatc gcggccgtgc ctactctgga
     4861 gatggctccg gccacggccg cgcctgcaag ttccagccgt ggaaacccga accagccgaa
     4921 gatgaaaatc ggatcgagcg caaggttgac caccgcgcca agggtcatca ccaggccggg
     4981 cacgttggcg cttccgctgg cgcgcatgat gttgccggcg gccatggtgg tggcgaaaaa
     5041 cacgacgctc ggatagtaga tgtcgaggta gctgtggatg agcggcagca tgtgtgcagg
     5101 ggcgccaaga gccagaaaga ccggatcgat agccagccaa ccaatgaaag acagagccga
     5161 tatcatggcc acgaccagca gcaggctgtg ggtcccgagg cgccggccat cctcaagctg
     5221 gccggcccca acgctgcggg cgattacgga ggacgtcccg attccgacac ccagcgcgat
     5281 gctcgaaagg atcatcacga ccggaaaggt gaaggtgatg gcggcgaccg attccgtacc
     5341 cagctgcccg atgaacccga tctcgagcat cgcgacgagg atactgctcg atacgccaag
     5401 catcatcggc gccgtcagac gaaacagtga tgtggcaact gggccttgtg tcagatccgt
     5461 gtggggcatt gagtatcagc cgagcgacgg tgcagtttaa gcgcaccgtg ccagggctcc
     5521 tagcgggagg gccgtttttc aggcagcggt tgcggccggt gagacggcgc agatgctgaa
     5581 gctaggtcgc cgcgggaaac tcgagaatga aggtggtgtg ctcgtcttcc gccgactcga
     5641 aggtaatgct gcccccgatg gattccatga ccatcttgca gaagctcaac ccgattcccg
     5701 caccctgtcc ggtgctggtg gtagtgtaga accgatcgaa cacctgactc ctggcgacgc
     5761 gggaaatacc tgatccggta tcgtgcacgg tgatgcagcg tcgagcacaa gagatcgtga
     5821 tcgagccctt gcctgctcgc tgcacgtagt agagagcatt cttgatcagg ttgaaaagga
     5881 cgtgaaccac cagcaggcga ggtgcgtgga tttcaaaatc ctcctcgacc gcgagagtaa
     5941 tgagcgcccg ctcgttagcg ttgttgaagg ggtaacgccc gaaactctct gacatgactg
     6001 aggacgccgt gaac
//
LOCUS       DBNHLM010000258         3761 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_276279, whole genome shotgun sequence.
ACCESSION   DBNHLM010000258 DBNHLM010000000
VERSION     DBNHLM010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_276279"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="AC16QF_12075"
     CDS             <1..263
                     /locus_tag="AC16QF_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449341.1"
                     /translation="TAPYMHNGVFRELRTAIHFYNRYLVRNPASMTNPETGEPWAPAE
                     VEETVDLDLLSEGQPLSDERIALLEAFLRTLTDERYEHLLEK"
     gene            343..783
                     /locus_tag="AC16QF_12080"
     CDS             343..783
                     /locus_tag="AC16QF_12080"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6449342.1"
                     /translation="MTSVANQEPIAEGLFTWPSNDPRLLGSRCDDCGVVTFPAQNACP
                     ACCGEDTTTIELERRGTLWTWTIQGFLPNRPPYDGPETPETFEPFGVGYVELPGQVRV
                     EARIKTDDLSKLKIGMEMELVVEKYVERDGKDIMAYFFKPADEL"
     gene            complement(801..1430)
                     /locus_tag="AC16QF_12085"
     CDS             complement(801..1430)
                     /locus_tag="AC16QF_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6449343.1"
                     /translation="MSLQAYRDARAREKRADILSAASRLFAERGFAQVSTAALAEEAG
                     VSTATLYRYFADKESLFVAVIDSLVADVIQQMADLPRASDDRLRELAFRYANLLCDPI
                     VVGIIRSVVADRQSTSAFRDSLESHGNEIFANEFDVEIRALLSAHDSDELDLAHQASI
                     ELRGALEHVTLLPELLFHEHLAEEELKGVVEQTLISWRRRWLGEQGSST"
     gene            complement(1440..2738)
                     /locus_tag="AC16QF_12090"
     CDS             complement(1440..2738)
                     /locus_tag="AC16QF_12090"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014579434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6449344.1"
                     /translation="MSEAHDVEPTSESTMLNKINPAELPYQDPWTTPLEELDVARPQL
                     FHADAQWPYFERLRKEAPVNYCNFSAFGPYWSVTSYDMIKAVDQNHEVFSSEPGITIE
                     DQDEDFQLPMFIAMDPPKHDVQRKAVQTAVAPRNLLEFESIIRERTASVLDGLPENED
                     FDWVEHVSINLTTMMLATLFDFPFEDRQKLTYWSDVVTGGVYTEFTQESQDAARADLL
                     QCLAYFTELWQERAKRPGGFDLISMLAHHPATKDLLTRPMEYLGNLVLLIVGGNDTTR
                     NSMSGGVYALNRFGDEYDKLRANENLIPNMVSEIIRWQTPLAHMRRRAKQDVELGGET
                     ISAGDKVVMWYVSGNRDESVFEEADRLIIDRANARQHLSFGYGIHRCMGNRLGEMQLR
                     ILWEEIQKRFSFVEVTGDPERSFSAFVKGYTKLPVRVHRR"
     gene            complement(2931..3710)
                     /locus_tag="AC16QF_12095"
     CDS             complement(2931..3710)
                     /locus_tag="AC16QF_12095"
                     /inference="COORDINATES: protein motif:HMM:NF018038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR6449345.1"
                     /translation="MAGRIDVHLVTGGRFHDTNFARLELLKLLAENDDLYVRVGEDFS
                     NESAIASADLLVSYTCDVRPSPDQQRWLKSFLERGGRWFALHGTNSLLDFDTTDGPIE
                     AFGVTIPGMPFAPDLTPDFMRLLGSRFVTHPPIQKFRVHVSAPDHPLVTGLDDFETED
                     EPYCCDFYDEVEVLLEARYSSEWMTELPAEWDDPGTPRPQMYLRRLGKGEVLYLTLGH
                     CCGKFDMQPIMEECDVVRCSWDRPVYYELLRRGIRWGSRTA"
BASE COUNT          734 a         1197 c         1148 g          682 t
ORIGIN      
        1 tgacggcccc gtacatgcac aacggggtgt ttcgcgagct ccgcacggcc attcatttct
       61 acaaccgcta tctggtgcgc aaccccgcga gcatgaccaa tccggagacc ggcgaaccgt
      121 gggcgcccgc cgaggtggag gaaaccgtcg acctcgacct gctcagcgag gggcaacccc
      181 tatccgacga gcgcatcgcg ctgctcgaag cgtttctgcg cacgctgacc gacgagcgat
      241 acgagcacct gctcgagaaa tgagcccgct tcccggccac ccggtgacaa acgccctccg
      301 gctctgctac aaaccatgtt cacatctttc gggaaagctt aagtgaccag tgttgccaac
      361 caggaaccca tcgccgaggg gctgttcacc tggccatcga acgatccgcg cctgctcggg
      421 agccgctgcg acgattgcgg cgtggtgacc ttccccgcgc agaatgcctg ccccgcctgc
      481 tgcggtgagg acacgacgac gattgagctc gagcgccggg gcaccctgtg gacctggacc
      541 attcagggct ttcttcccaa ccgcccaccc tacgacggcc ccgagacgcc ggaaaccttc
      601 gagccgttcg gagtgggcta cgtcgagctg cccggccagg tgcgggtgga ggcacgcatc
      661 aaaacggacg atctctccaa gctgaagatc ggcatggaga tggagctcgt ggtcgagaaa
      721 tacgtcgagc gcgacggcaa ggacatcatg gcttacttct tcaagccggc ggacgagctc
      781 tagaccaccc gcttcttcag tcaggttgaa gagccctgct ctccgagcca gcgcctgcgc
      841 caggaaatca gcgtctgctc gaccactcct ttgagctctt cttcggccag gtgctcgtgg
      901 aacagcagct caggcaggag tgtcacgtgc tcgagcgcgc cccgcagctc gatgctcgcc
      961 tgatgcgcca gatccagctc gtcgctgtca tgggcgctca gcagcgctct gatctcgacg
     1021 tcgaactcgt tggcgaatat ctcgttgcca tggctctcca gactgtcacg gaacgcgctg
     1081 gtgctctgcc gatcggccac aacggaccgt atgatgccga cgacgatggg gtcgcagagc
     1141 agattcgcat agcggaacgc gagctcacga agccggtcat cggacgcgcg cggcaggtcg
     1201 gccatctgct ggatcacgtc ggccaccagg gagtcgatga cggcaacgaa cagagactct
     1261 ttgtctgcga agtaacgata tagcgtcgcc gtcgacacgc ccgcttcctc ggccagcgct
     1321 gccgtggaca cctgtgcgaa gccgcgctca gcaaacaggc gcgacgcggc gctcaggatg
     1381 tcggcccgct tctcgcgggc ccgcgcgtca cgataggctt gcagagacat ggcgagtgat
     1441 cagcgccgat gcacgcgcac cggcagcttc gtgtatccct tgacgaacgc ggagaaggag
     1501 cgttccgggt cgccggtgac ctccacgaag gagaaccgct tctggatctc ttcccagagg
     1561 atgcgcagct gcatctcgcc cagacgattg cccatgcagc gatgaatgcc atagccgaag
     1621 gagagatgct gacgggcatt cgcgcgatcg atgatgaggc ggtcggcctc ctcgaatacg
     1681 ctctcgtccc gattgccgga cacgtaccac atgaccacct tgtccccggc ggagatggtc
     1741 tccccaccta gctccacgtc ctgcttggcc cgccgacgca tgtgggccag cggcgtctgc
     1801 cagcggatga tctccgagac catgttcggg atgaggttct cgttggcccg cagcttgtcg
     1861 tactcgtcac cgaacctgtt cagggcgtaa acgccgccgc tcatggagtt tcgcgtggtg
     1921 tcgttgccgc cgacgatcag cagcaccaga ttgcccagat actccatggg acgggtgagc
     1981 agatccttgg tggccgggtg gtgggccagc atcgagatga ggtcgaaacc accaggccgc
     2041 ttggcccgct cctgccacag ctcggtgaag taggccaggc actgcagcag gtcggcccgg
     2101 gccgcatcct gggattcctg ggtgaactcg gtatacacgc cgcccgtcac cacgtcagac
     2161 cagtaggtca gcttctgacg atcctcgaaa ggaaagtcga acagcgtcgc cagcatcatg
     2221 gtggtgaggt tgatggagac gtgctcgacc cagtcgaagt cctcgttctc tggcagcccg
     2281 tccagcacgc tggccgtgcg ctcacggatg atggactcga attccagcag gttgcgcggc
     2341 gccacggcgg tctggacggc cttgcgctgc acgtcgtgct tgggcgggtc catggcgatg
     2401 aacatgggca gctggaagtc ctcgtcctga tcctcgatgg tgatcccagg ctccgagctg
     2461 aacacctcgt ggttctgatc caccgccttg atcatgtcgt agctcgtcac cgaccagtac
     2521 ggcccgaagg cgctgaaatt gcagtagttg acgggcgctt ccttgcgcag acgctcgaag
     2581 tagggccatt gggcatctgc gtgaaacagc tggggacggg ccacgtccag ctcttcgagc
     2641 ggcgtggtcc acggatcctg ataaggcagc tccgccgggt tgatcttgtt cagcatggtg
     2701 gactcggagg tcggttcgac gtcgtgggct tcgctcatga ggactcctga aagcgtttgt
     2761 ctgactgacg cgggcaatgc acggttggcc cgcggccgaa aacgtgaatt ctaaactaaa
     2821 ctggtcagtt tacaattacc atcagaaaaa ccgccaccag gtacacagaa tccaaggagc
     2881 cgggcatcag atctgcacca ccaaggaggt atcgcgcgtg ctcaggacac tcaagccgtc
     2941 cggcttcccc agcgaatgcc gcgccggaga agctcgtagt agaccgggcg gtcccaactg
     3001 cagcggacga cgtcgcactc ttccatgatc ggctgcatgt cgaacttacc gcagcagtgc
     3061 ccgagcgtca ggtaaaggac ctcccccttc cccaggcgcc gtaagtacat ctggggccgc
     3121 ggggtaccgg gatcgtccca ctccgcaggc agctccgtca tccactcgct cgagtagcgg
     3181 gcttccagca gcacctcaac ctcgtcgtag aagtcgcagc aatagggttc gtcctccgtc
     3241 tcgaagtcgt ccagcccggt cacgagcgga tgatccggcg cgctgacgtg cacgcgaaac
     3301 ttctgaatcg gcggatgggt gacgaatcga ctgcccagca gacgcatgaa gtcaggggtg
     3361 agatccggcg cgaacggcat cccgggaatg gtcaccccaa acgcctcgat gggaccatcg
     3421 gtggtatcga aatcgagaag cgagttggtc ccgtgcagcg cgaaccatcg tcctcctctt
     3481 tcgagaaagg atttcagcca acgctgctgg tcggggctgg gacgcacatc acaggtgtag
     3541 ctgaccagca gatcggcaga cgcgatcgcg ctttcgttgc tgaaatcctc acccaccctg
     3601 acgtagagat cgtcgttctc cgccagcagc ttcagaagct ccaggcgggc aaagttggtg
     3661 tcgtgaaagc gaccaccggt cacgaggtgg acgtcaatgc gcccggccat taccacgttc
     3721 cttcaggtac ggatccaaat cggctttcca gtgaccagca g
//
LOCUS       DBNHLM010000259        14653 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_516102, whole genome shotgun sequence.
ACCESSION   DBNHLM010000259 DBNHLM010000000
VERSION     DBNHLM010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 14653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14653
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_516102"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /gene="gspL"
                     /locus_tag="AC16QF_12100"
     CDS             complement(<1..893)
                     /gene="gspL"
                     /locus_tag="AC16QF_12100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HIR6449346.1"
                     /translation="MPKLFIRALTPATRDDEGYHLEAEWLITEDDGSIRANGQTDLRG
                     LAELVDPETDWLQTADNLVVIVPGDYVLGVSCEVPGRSAGQIRRALPFVVEEFVATDI
                     EAMHLAAGEIQRGEPVRCCLVDRSLLEDWLVCLSEIGLSPGHMTAEAELLPAAEGQVS
                     VLLEARRALLKTLDQSATVDRSNLMMALESLTADKLLLVNGDPAALDFELERLEGLTI
                     LTGDEDAEAGSTLAYLANRWLADGDGVNLLQGAYLPKRPVNPVWARWQGVAALAAAWA
                     GVGIIAMIVEGLWAGAQADRL"
     gene            complement(955..1926)
                     /gene="gspK"
                     /locus_tag="AC16QF_12105"
     CDS             complement(955..1926)
                     /gene="gspK"
                     /locus_tag="AC16QF_12105"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HIR6449347.1"
                     /translation="MDRPQRVSHPPSRSRGVVLLSILLILALLSALGYQMVGRHGLTV
                     AQVRHTFMHDQALAYALGAEAFARQVLYEDWSDTGPGSDNLQEAWAQPLAPFEIEDGL
                     LEVQIRDLNGCFNLNSLAGAQTEDNLNRLKTLLRNAGVPDAMADTWRDWVDADEEVSG
                     FGAEDASYLLDDTAYRTANRMAGHISELMLLKDASLDFVEALRGSACLLPSDELRLNV
                     NTASADALAALSPDLAPAQMESFVMSERSYEEVGEVTEEFPELAGSAGVLSVVSEYFE
                     VQVRAEVGETRAELASIVHRDPNDGSMTLIARDFGRDFRSRFEEEEG"
     gene            complement(1905..2531)
                     /gene="gspJ"
                     /locus_tag="AC16QF_12110"
     CDS             complement(1905..2531)
                     /gene="gspJ"
                     /locus_tag="AC16QF_12110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HIR6449348.1"
                     /translation="MSAWIAPLTARRQDGFTLLEMLVVFSVFAVLGVVASQIVGRVLG
                     NQQILSERGERLAEIQRAMEIIQRDVMQVSSRGVRDQLGDPMDPLMIGADGLMEFTRT
                     GWRNPLGRRRAELQRVTYMMQDGDLYRAYWPVLDRAADTEPNLQQLLRGVEQIEFFAV
                     DLDGNEHSFWPLLDRSDSESGSSLGAIMMRIDLNPFGIVERLWTVPSV"
     gene            complement(2531..2911)
                     /gene="gspI"
                     /locus_tag="AC16QF_12115"
     CDS             complement(2531..2911)
                     /gene="gspI"
                     /locus_tag="AC16QF_12115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HIR6449349.1"
                     /translation="MGQRGFTLVEVLVALVVFAVMGFTITTRVGDVSSQMFGMERRTM
                     AHWVADNHLNRMRLAQLAADEVLATGLDRERVFMGDREWRLEVDVADTTHPWLRRVEI
                     TVFEATESGEVGPLDHVVAFLGRY"
     gene            complement(2911..3459)
                     /gene="gspH"
                     /locus_tag="AC16QF_12120"
     CDS             complement(2911..3459)
                     /gene="gspH"
                     /locus_tag="AC16QF_12120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HIR6449350.1"
                     /translation="MPTSSSRIFSRPAVRQRGFTLLELLVVVSLVGILTGVVVLGFTG
                     ADTQQEIKGLAQTMAYRVELARQQALTRNREWGIYVDEQAYRFAEYRPEEGEWEDQSR
                     RPFDVIDVPEHVSLRLETEGVGELPFADGERLPQILVFSSGEITPFTVYLEFGDARDP
                     WVVSSDGLARARASRSSEIGEA"
     gene            complement(3431..3850)
                     /gene="gspG"
                     /locus_tag="AC16QF_12125"
     CDS             complement(3431..3850)
                     /gene="gspG"
                     /locus_tag="AC16QF_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HIR6449351.1"
                     /translation="MKARIAGFTLIEVLVVVVIIGILGAVIVPNLLGRPDQARVTAAQ
                     SDIRSLANALDIYRLDNFQYPSTDQGLEALESKPTGFPEPKNYNPEGYIKKLPADPWG
                     SPYVYERTEEGFALYSYGADGQEGGEGLNADIFFEDL"
     gene            complement(3859..5079)
                     /gene="gspF"
                     /locus_tag="AC16QF_12130"
     CDS             complement(3859..5079)
                     /gene="gspF"
                     /locus_tag="AC16QF_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HIR6449352.1"
                     /translation="MAAFEYTALDPRGRQRKGVLEADSVRQIRQLLRDQGLVPLGVEA
                     AGERAVKKPGEGFSFKLPRRIGPLDRVLFTRQLATLIAAGLPVEEALQAVAQQSEKQH
                     IRSLVMGIRSRVLEGHSLASSLASYRSSFSEMYRSTVAAGEQSGHLDKVLDNLANYTE
                     RQFEARRDVEMAMLYPAVLTLLAFGIVGALMVYVVPDMVSVLENMGQGLPASTRFLIA
                     TSELARDYWWALIALVGLVVAGIRWALAQPPIRLAWDRQKLFMPLVSRITRSSNAARY
                     ANTLSILTSSGVPLVEAMNIASEVVSNAWLKRRLADSTQRVSEGTSLRAALEGVGHFP
                     PMLLHMVASGESSGELDSMLEKVAIYQQTEVERIVTTLVRLFEPLMLVVMGGLVMFIV
                     MAILLPILNMNQLV"
     gene            complement(5087..6607)
                     /gene="gspE"
                     /locus_tag="AC16QF_12135"
     CDS             complement(5087..6607)
                     /gene="gspE"
                     /locus_tag="AC16QF_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HIR6449353.1"
                     /translation="MSDLAHDEPDADIAQVQDAPLPLPFAFARRFGVLITGETGSREQ
                     ALVACKVQPSLTTLAEIKRVVRRELKLKMVGQDEFERLLTATYARDGSAARQMVEDMG
                     DEMDLASLADSVPETEDLLEQEDDAPIIRLINALLAEAIRENASDVHIETFEKDLIVR
                     FRVDGVMREVVKPKRQLAPLLVSRIKVMARLDIAEKRVPQDGRISLRIGGREVDVRVS
                     TMPASNGERVVLRLLDKQAGRLRLENLGMSNEGLGLLRDIVHKPHGIFLVTGPTGSGK
                     TTTLYASLAELSTQTINILTVEDPIEYNLPGIGQTQVNTKADMTFARGLRAILRQDPD
                     VVMVGEIRDIETAEVAVQASLTGHLVMSTLHTNTAVGAITRLIDMGVEPFLLSSSLVG
                     MLAQRLVRVLCPKCKEHRSAMASELEFLDQPSAFVYEARGCDACQGTGYRGRTGIYEL
                     VPVDDDMRQLIHDQVSEQELTAFARQRTPGIRDDGKMKVLSGVTSVQEILRVTLEE"
     gene            6796..7812
                     /locus_tag="AC16QF_12140"
     CDS             6796..7812
                     /locus_tag="AC16QF_12140"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF006046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HIR6449354.1"
                     /translation="MDFVGSHILSVDQFTREDIEHVFRVADDMQPYADRERVTRVLQG
                     AILSNMFFEPSTRTRISFGSAFNMLGGEVRETTEVAASSLTKGESLYDTARVLSGYSD
                     VIVMRHPEAGSVAEFAKASRVPVINAGDGPNEHPSQALLDLYTIRKEMESRGRAIDDL
                     RIALVGDLRYGRAVHSLCKLLALFEHVHYKLVSPPELSLPADIVASLRAAGHDVEEHH
                     ELETGIDHVDILYVTRTQEERFPDQEEADRYRGLFRVNQAIYTKNCEPNTVIMHPLPR
                     DSRAQAQELDDDLIENPNLAIFRQTDNGVLIRMALFALVLDVAGQVHNHAQPVTWYTA
                     KRFR"
     gene            complement(7809..8246)
                     /locus_tag="AC16QF_12145"
     CDS             complement(7809..8246)
                     /locus_tag="AC16QF_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449355.1"
                     /translation="MAVYELNNGQLLRVARSIDGKLEQRYFSLIGLNKTQQRGVRREA
                     KAQDQAWQEAQEKAKAKRVREASTDRRHSTGVRGINLVRRPSPAFRVQVQTNGRSHVR
                     EFSVKRLGAEKAWRQATRFLAECRGYKTVPKAWTASVPRIPKG"
     gene            complement(8317..9246)
                     /locus_tag="AC16QF_12150"
     CDS             complement(8317..9246)
                     /locus_tag="AC16QF_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010563760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR6449356.1"
                     /translation="MSVLVVADHDNAELKPVTLVVVAAATAIGGDIDILVAGEGCGAV
                     AETAAKIPGVNKVLLADNAAYGHQLAENVAPLVAEIGAGYSHILTAHTTVGKNFLPRV
                     AAVLDVAQISDIQGVVGPDTFERPIYAGNAVATVKSNDAIKVLSVRGTAFDPVAAEGG
                     SASIESVASAHDAGVSSFVGEELAKSERPELTAASIIISGGRGMQSGENFVLLEGIAD
                     KLGAAIGASRAAVDAGFVPNDMQVGQTGKIVAPDLYIAVGISGAIQHLAGMKDSKVIV
                     AINKDEDAPIFQVADYGLVADLFKALPELESQI"
     gene            complement(9243..9995)
                     /locus_tag="AC16QF_12155"
     CDS             complement(9243..9995)
                     /locus_tag="AC16QF_12155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR6449357.1"
                     /translation="MKVLVPVKRVVDYNVKIRVKPDNTGVDLNNVKMSLNPFCEISVE
                     EAVRLKEAGTATEVVAVAVGGTVCQEQLRTCLALGADRAVLIETDQEPQPLGIAKALK
                     ALQERENADIVIFGKQSIDGDNAQTGQMFAALTGMPQGTFASELTVADGKAQIVREVD
                     GGLQTVSVTLPCVVTTDLRLNEPRYASLPNIMKAKKKPLDVVTPDELGVNVAPTVETL
                     SVKPPAERQAGIKVGSVEELVDKLKNEAKVIS"
     gene            10257..11912
                     /locus_tag="AC16QF_12160"
     CDS             10257..11912
                     /locus_tag="AC16QF_12160"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019395839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HIR6449358.1"
                     /translation="MERESMDFDVVIVGGGPSGLAAACRLMQLAESSGQELSVCLVEK
                     GSEIGAHILSGAVFEPTALNELFPDWEERGAPLDNPVTLDEVYYLVNEENRLKMPGFA
                     VPKVTHNHGNHAISLGNLCRWLGEQAENMGVNVFPGFAAAEILYGEDGAVAGVATGDM
                     GVGADGEHKATYAPGYELRGKYTIFAEGCRGHLGKQLMAKFDLQRDSATQHYAIGFKE
                     LWDVTPENHVPGKVVHTMGWPGRHLKGMFTGSYLYHLPNQQVSLGLIVDLSYSNPHVS
                     PFDEFQRWKHHPLIAETLSGGKRVSYGARAIVKGGLSALPKLSVPGALLVGDDAGFLN
                     MTKIKGSHTAMKSGMLSAEAVFESLSGGSAGGEDLSSFQEKFDSSWLHEELSAARNSQ
                     QAIHKFGTLAGAAFIWVDQTLFRGRLPFTLKDPTPDHATLKPAAQSRRIDYPKPDGVL
                     SFDKLSSVFLSNTNHEEDQPCHLVLADPTIPISQNLPTYDEPAQRYCPAGVYEVVEEN
                     GGARFQINAQNCVHCKTCDIKDPAQNINWVVPEGTGGPNYPNM"
     gene            complement(11957..12499)
                     /locus_tag="AC16QF_12165"
     CDS             complement(11957..12499)
                     /locus_tag="AC16QF_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007253383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HIR6449359.1"
                     /translation="MTDTSSIFEAIVSAQRDRRLPPVASWNPARVGHIDIRIASDGTW
                     YHEGEPIRRKNMVDLFSTVLRRDSDGYCLVTPAEKLLITVEDAPFVAVDFEARGESGG
                     QDLVFVINTGDAVVADSAHPVRVEDHGGVPRPYVMVRDGLEALIARPVFYRLVELAVE
                     EAGELCLYSRGERFVLGSVS"
     gene            complement(12557..13522)
                     /locus_tag="AC16QF_12170"
     CDS             complement(12557..13522)
                     /locus_tag="AC16QF_12170"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010208395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990140 - molybdopterin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061798 - GTP 3',8'-cyclase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR6449360.1"
                     /translation="MIVDALGRRFRNLRVSLTAACNYACSYCVPDGKRLQSAQHELDG
                     EELAYAVELVVDAAGIEQLRITGGEPLLSPKFDEVLPRLMALPLNDVSITTNGQLVPR
                     KIDLLLESGLKRINVSLDTLDPLRFRDIARGGDLETVLGGIGALAEGGLKVKINMVPM
                     RTANLDQLVPMLEFCLERGFELRYIELMNMGHLRQGNQYLRDFVGMEEILETIGKRYE
                     FTRTDAPYDSTAVRYEIPDRGFFGIIANETEPFCSSCTRLRLSSNGFIYGCLSNTARH
                     DVKPLLALPRHQAIAQLQGVLVEALADKQDLGFTGEVTVMKFIGG"
     gene            complement(13793..13981)
                     /locus_tag="AC16QF_12175"
     CDS             complement(13793..13981)
                     /locus_tag="AC16QF_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449361.1"
                     /translation="MANTHLNGRTFNYEGHCYLIVEDNDWEGSELKVKRLDGTRAQTS
                     LPTENVMRCVFPQKPDDR"
     gene            complement(14047..>14653)
                     /locus_tag="AC16QF_12180"
     CDS             complement(14047..>14653)
                     /locus_tag="AC16QF_12180"
                     /inference="COORDINATES: protein motif:HMM:NF014874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR6449362.1"
                     /translation="VRGQTADYLIGGEPFTAHVPGGGEHRAASLAAVLATLKVLDRPV
                     RDGLQLGDDLVPKGRGNETLINGVTVVDDSYNANPESMKAALRTLAAVPGRKVAVLGD
                     MLELGAEGPAHHRGLAEACSGIDRVYCVGEAMRELFDALPEGQRGSWLPDAGGEGLID
                     EILEDLRSGDRVLFKGSNRIFWAKGLVNRFSERLHTFTMSD"
BASE COUNT         2612 a         4841 c         4566 g         2634 t
ORIGIN      
        1 tgcagccggt cggcctgggc gccggcccac aaaccttcga cgatcatggc gatgatgccc
       61 acgccggccc aggcggccgc gagcgcggcc acgccctgcc agcgcgccca aacggggttg
      121 acgggccttt tcgggaggta agcgccctgc agcagattga ccccgtctcc gtcggccagc
      181 cagcggttcg cgaggtaagc aagggttgaa ccggcttccg cgtcctcatc gccggtcagg
      241 atggtgaggc cttcgagccg ttcgagctcg aaatccagtg cggccggatc gccgttcacg
      301 agcagcagct tgtctgccgt caggctttcg agcgccatca tcagattcga gcggtcgacg
      361 gtggcggatt gatcgagcgt cttcagcaat gcccgccgag cctcgaggag cacgctcacc
      421 tgtccctcag ccgccggcag cagctcggct tccgcggtca tgtgcccggg agagaggccg
      481 atctccgaaa ggcagacgag ccagtcctcg agcagagaac ggtccaccag acagcagcgg
      541 acgggctcgc cgcgctggat ctcaccggca gccagatgca tggcctcgat gtccgtggcg
      601 acgaactcct ccaccacgaa gggcagcgcc cggcggatct gccccgcgct ccggccaggc
      661 acctcacagg aaacgccgag cacgtaatcg ccggggacga tgacgacgag attgtccgcc
      721 gtctggagcc agtctgtctc cggatcgacg agctccgcga gcccacgcag atcggtctga
      781 ccgttcgctc ggatagagcc gtcgtcctcg gtaatcagcc actcggcctc gaggtggtag
      841 ccctcgtcat cgcgcgttgc cggcgtgagc gctcgaatga acagctttgg catttacttg
      901 gctccggtct tggtgtttga cttcatacgt tttcctggtc ggaagcagcg ctcctcaacc
      961 ttcctcctcc tcgaagcggg atctgaagtc ccggccgaaa tcacgggcga tcagcgtcat
     1021 cgagccgtcg ttgggatcgc gatggacgat ggacgcaagc tcggcccggg tctctcccac
     1081 ctccgccctc acctgcacct cgaaatactc gctcaccacg ctcaggacgc ccgcggagcc
     1141 ggcaagctcc ggaaactcct cggtcacctc gcccacttcc tcgtagctgc gttcggacat
     1201 cacaaagctt tccatctggg ccggcgcgag atcggggctc agcgcggcaa gggcatctgc
     1261 gcttgccgtg ttcacgttga gccgcagctc gtcggatggc agaaggcagg cgctgcctcg
     1321 cagggcctcg acaaagtcca atgacgcatc cttgagcagc atcagctccg agatgtgtcc
     1381 tgccatgcgg ttggccgtcc gataggccgt atcgtccaga agatagctcg cgtcctccgc
     1441 gccgaatccg gaaacttcct cgtccgcgtc gacccagtcc cgccaggtgt ccgccatggc
     1501 gtcaggtacc cccgcgttgc gcaacagggt cttcaggcga ttcagattgt cctcggtctg
     1561 ggcaccggcg aggctgttga ggttgaagca gccgttcagg tcgcggatct gcacttccag
     1621 cagcccgtcc tcgatctcga aaggcgccag cggctgagcc caggcttcct gcaggttgtc
     1681 cgatccggga ccggtgtcgc tccagtcctc gtaaaggacc tgccgtgcga acgcttccgc
     1741 gccaagggcg tatgccaggg cctggtcgtg catgaaggtg tgccggacct gggccaccgt
     1801 gaggccgtga cggcccacca tctgatagcc cagtgcagag agcagcgcca gaatcagcag
     1861 tatgctgagc agcacgacgc cgcggcttcg cgacggcgga tggctcacac gctggggacg
     1921 gtccatagcc tttccacgat gccaaatggg ttcaggtcga tgcgcatcat gatcgcgccc
     1981 aggctcgagc cggactcact gtccgagcgg tccagcagcg gccagaaact gtgctcgttg
     2041 ccgtcgaggt ccacggcaaa gaactcgatc tgctccacgc cgcgcagaag ctgctgcagg
     2101 ttgggctccg tatccgccgc gcggtcgagc accggccagt aagcgcggta aaggtcgccg
     2161 tcctgcatca tgtaggtcac ccgctggagc tctgcgcgac ggcggccaag ggggttccgc
     2221 cagccggttc gagtgaattc catcaaaccg tctgcgccga tcatgagcgg atccatgggg
     2281 tcgcccagct gatcgcgcac gccgcggctg ctgacctgca tcacgtcgcg ctgaatgatc
     2341 tccatggcgc gctggatctc ggccaggcgc tcgccacgct cgctcagaat ctgctggttg
     2401 ccgagcaccc gaccgacgat ctggctggcc accacgccca gcacggcgaa aacactgaat
     2461 accaccagca tttccaggag ggtgaagcca tcctgccgcc gtgccgtgag tggagctatc
     2521 caagccgaca tcagtagcgc cccagaaagg ccacgacgtg gtccagaggt ccgacctccc
     2581 ccgactccgt cgcctcgaac accgtgatct ccacgcgacg cagccaggga tgcgtggtgt
     2641 cggccacgtc gacttcaaga cgccactccc gatcccccat gaatacccgt tcgcgatcaa
     2701 ggcccgtcgc cagaacctcg tcggcggcca gctgggccag gcgcatgcgg ttcagatgat
     2761 tgtccgccac ccaatgggcc atggtgcgtc gctccatgcc gaacatctgg ctggagacgt
     2821 cgccgacgcg agtggtgatg gtgaatccca tcacggcgaa gaccaccagc gccacgagga
     2881 cctcgaccag ggtgaatccg cgctgcccca tcaagcctct ccgatctcgc tggacctgga
     2941 ggcgcgggcg cgcgcgagcc cgtctgagct gacgacccag gggtcacgag cgtcgccgaa
     3001 ctcgagatag acggtgaacg gcgtgatctc gccggaggag aacaccagga tctgcggcag
     3061 cctctctccg tccgcgaagg gcaactcccc taccccctcg gtttccagcc gcaggctcac
     3121 gtgctcggga acgtcgatga cgtcgaacgg gcgccgggac tgatcttccc actcgccctc
     3181 ttcgggccga tactcggcga accgatacgc ctgctcgtcc acgtagatgc cccattcgcg
     3241 attgcgggtc agcgcctgct gacgggcgag ctccacgcgg taagccattg tctgggcgag
     3301 ccctttgatc tcctgctgag tgtctgcgcc cgtgaaaccc agtacgacca caccggtgag
     3361 gatcccgacc aggctcacca ccacgagcag ctccagcagg gtgaagccgc gctgacggac
     3421 cgccggccgg ctaaagatcc tcgaagaaga tgtcggcatt gagtccctct ccgccttcct
     3481 ggccgtctgc gccgtacgag taaagcgcaa agccctcctc ggtgcgctcg tagacgtagg
     3541 gagagcccca gggatccgcg ggaagcttct tgatgtagcc ctcggggttg tagttcttgg
     3601 gctccgggaa ccccgtaggc ttggactcca gggcttccag gccctggtct gtggacgggt
     3661 actggaagtt gtccagccga tagatgtcaa gcgcgttggc cagggaccgg atgtcgcttt
     3721 gggccgccgt gacgcgcgcc tggtcgggcc ggccaagcag gttcgggacg atgacggcgc
     3781 ccaggatgcc gatgatgacc accaccacca gcacctcgat cagcgtgaaa cccgcaatcc
     3841 ttgctttcat cttcaccgtc acaccagctg gttcatgttg agtatgggca gcagaatagc
     3901 catcacgatg aacatgacaa ggccccccat cacaaccagc atcagcggtt cgaaaagtct
     3961 cacaagggtt gtgacgatcc gctcaacctc agtctgctga taaatggcca ccttttcgag
     4021 catgctgtcg agctcgccgc tggactcgcc gctggccacc atgtgcagca gcatgggcgg
     4081 gaaatgcccc acaccctcca gcgccgccct gaggctcgtt ccttcgctca cacgctgggt
     4141 tgagtcggca agccgccgct tgagccacgc attggaaacc acctcgctgg cgatgttcat
     4201 ggcctctacg agcggcaccc cgctgctggt caggatgctc aaggtatttg cgtaacgggc
     4261 cgcgttgctg ctcctcgtga tgcggctcac gaggggcatg aagagcttct gccggtccca
     4321 ggccagacgg atgggcggct gggcgagcgc ccatcgaatc ccagcaacca ccagtcccac
     4381 caaggcgatg agcgcccacc agtaatcccg tgccagttct gaggtagcga tgaggaaacg
     4441 ggtgctggcc ggcagcccct gccccatgtt ctcgagcacg ctgaccatgt ccggcaccac
     4501 gtagaccatc agcgcgccga cgatgccgaa ggccagaagg gtcagcaccg ccggatagag
     4561 catggccatt tccacgtccc ggcgggcctc gaactgccgc tccgtgtagt tggcgaggtt
     4621 atcgagcacc ttgtcgagat gccccgactg ctcgccggcg gcaaccgtgg agcggtacat
     4681 ctcggaaaag ctgctacggt agctggcgag gctcgaggcc agcgagtggc cttccagcac
     4741 tctcgagcga atgcccatga ccagggagcg tatgtgctgc ttttcggatt gctgggccac
     4801 cgcctgaagg gcctcctcga cgggcaggcc tgcggcgatg agggtagcga gctgacgtgt
     4861 gaacagcacg cggtccagcg gtccgatccg acgcggcagc ttaaaggaga aaccctctcc
     4921 cggtttcttg acggcgcgtt ccccggcggc ttccacgccg aggggaacca gaccctggtc
     4981 gcgcagcagc tgacggatct ggcggacgct gtccgcctcc aggacgccct tgcgttggcg
     5041 ccctctggga tcaagcgctg tgtactcgaa tgccgccatg tcggggctat tcctccaggg
     5101 tgaccctcag gatttcctgc accgaggtca cgccggagag caccttcatc ttgccgtcgt
     5161 cacggatgcc tggcgttctc tggcgcgcaa aggccgtgag ctcctgctcg gagacctgat
     5221 cgtgaatcag ctggcgcatg tcgtcgtcca cggggaccag ctcgtagatg cccgtgcggc
     5281 cgcgatagcc ggtgccctgg cacgcatcgc agccgcgcgc ctcgtaaacg aacgcggaag
     5341 gctggtcgag gaactcgagc tccgaggcca tggcgctgcg gtgttccttg cacttcggac
     5401 agagcacgcg gacgagccgc tgcgccagca tccccaccag gctcgaggac agcaggaagg
     5461 gctcgacgcc catgtcgatc aggcgggtaa tggctcccac ggccgtgttg gtgtggaggg
     5521 tggacatcac gaggtgcccc gtgaggcttg cctggacggc gacctcggcg gtctcgatgt
     5581 cccggatctc gccaaccatc accacgtcgg ggtcctgacg cagaatggcg cgaaggccgc
     5641 gggcgaaggt catgtccgcc ttggtattca cctgggtctg gccgatgccg ggcaggttgt
     5701 actcgatggg gtcctcaacc gtcaggatgt tgatggtctg ggtgctcagc tcggccaggg
     5761 aggcgtagag cgtggtggtc ttacccgacc cggtggggcc ggtgaccagg aagatgccgt
     5821 gaggcttgtg aacgatgtcg cgcagcaacc cgagcccttc gttcgacatg cccagatttt
     5881 ccagcctcaa ccgtcctgcc tgcttgtcca gaagccgaag caccaccctt tcaccgttgc
     5941 tggccggcat ggtggaaacc cgcacgtcga cttcccggcc gcctatgcgc agggagatcc
     6001 ggccgtcctg aggaacccgc ttctcggcga tgtccaggcg ggccatgacc ttgatccggg
     6061 agaccagcag aggcgccagc tggcgcttgg gtttgaccac ttcccgcatc accccgtcga
     6121 cccggaaccg gacgatgagg tccttctcga acgtctcgat gtgaacgtcg gaagcgtttt
     6181 cgcgaatggc ctcggccagc agcgcgttga tgagccggat gatgggcgca tcgtcctcct
     6241 gctcgagaag gtcttcggtc tccgggaccg agtcggcaag gcttgcgaga tccatctcgt
     6301 cgcccatgtc ctcgaccatc tggcgggcgg cggagccgtc ccgggcgtag gtcgccgtga
     6361 gcagtctttc gaactcgtcc tgacccacca tcttcagctt cagctcgcgc cgcaccacgc
     6421 gcttgatctc ggccaaggtg gtgaggctcg gctggacctt gcaggctacc agggcctgct
     6481 cccgggagcc ggtttctccc gtgatcagaa cgccgaatcg gcgggcaaag gcgaaaggca
     6541 ggggcaacgg cgcgtcctgc acctgagcta tgtcggcgtc cggctcgtca tgtgccaagt
     6601 cgctcatctg gcggtactcg tactggggca gcccaatgat agcaacccag gcacgcaccg
     6661 ctaaacccaa agattccaac ccgtgtggcg ctgcccttgc ttccgtgacg gtcatcacaa
     6721 agcgtgaacg gcggctcgtt ttcccgtgac gccgcggtag gtgtagactt gcgcaaattc
     6781 agccggggga cgccgttgga cttcgttggc agccatatac tgtcggtgga ccagttcact
     6841 cgtgaggaca tcgagcacgt tttccgggtc gcggacgaca tgcagcctta tgcggatcgg
     6901 gaacgggtca cccgggtcct gcagggcgcc attctctcca acatgttttt cgagccgagc
     6961 acccggaccc gcatctcgtt cggctcggcg ttcaacatgc ttggcggcga ggtacgcgag
     7021 accactgagg tagccgcgtc gtcactgacc aagggggaat ccctttacga caccgcccgc
     7081 gtcctctccg gctacagcga cgtcatcgtg atgcgccatc ccgaggccgg atcggtggcg
     7141 gagttcgcca aggccagccg ggtgcccgtc atcaacgccg gagacggtcc caacgagcat
     7201 cccagccagg cgctgctgga tctctacacc atccgcaagg aaatggagag ccgcggccgc
     7261 gccatcgacg atctccggat cgcgctggtg ggtgacctgc gctacggacg ggccgtgcac
     7321 tccctctgca agctgctcgc gcttttcgag cacgtccact acaagctggt gtccccgccc
     7381 gaactttccc tgccggcgga catcgtggcg tcgctcagag cggccggcca cgacgtggag
     7441 gagcaccacg agctggagac cggcatcgat cacgtggaca tcctctacgt gacgcgcacc
     7501 caggaagaac ggtttcccga ccaggaagaa gccgaccgct accgcgggct gttccgggtc
     7561 aaccaggcca tctacacgaa gaactgcgaa cccaacacgg tgatcatgca tccgctgcca
     7621 cgggactccc gcgcccaggc tcaggagctg gacgacgatc tgatagaaaa ccccaatctg
     7681 gccattttcc gccagacgga caacggggtt ctgatcagga tggccttgtt tgcgctggtg
     7741 ctggatgtgg ccggccaggt gcacaaccac gctcagccgg tgacgtggta tacggccaag
     7801 cggttccgct agcctttggg gatacgaggc acgctggcgg tccaggcctt cggcacggtc
     7861 ttgtagccgc ggcactccgc tagaaagcgg gtcgcctggc gccaggcctt ctcggcgccg
     7921 agccgcttga ccgagaactc ccggacgtga gagcgaccgt tggtctgaac ctgcacccgg
     7981 aaggcgggtg agggcctgcg caccaggttg atgccgcgca cgcccgtgct gtgcctgcga
     8041 tcggtagagg cctcccgcac gcgcttggcc ttggccttct cctgagcctc ctgccaggcc
     8101 tggtcctgtg ctttcgcctc gcgacgcacg ccccgctgct gggtcttgtt cagcccgatg
     8161 aggctgaagt aacgctgctc gagcttgccg tcgatgctgc gggcaacccg cagaagctgg
     8221 ccgttgttga gttcgtaaac cgccaaaata ctaactcctc gctaaggaac ctcgcaaaga
     8281 caggttccgt agccgtcaaa tgtttctgta cgcgcgtcag atctgggatt ccagctccgg
     8341 cagcgccttg aagagatcgg ccacaaggcc gtagtcggcc acctggaaaa tcggggcgtc
     8401 ttcgtccttg ttgatggcca cgatcacctt gctgtccttc atgcctgcaa gatgctggat
     8461 ggcgccggaa atgcccacgg caatgtagag atcgggtgcc acgatcttgc cggtctggcc
     8521 cacctgcatg tcgttgggga cgaagccggc gtccactgcg gctcgggacg cgccgatggc
     8581 agcgccgagc ttgtcggcta taccttcgag cagcacgaag ttttccccgc tctgcatgcc
     8641 gcgcccgccg gagatgatga tgctcgccgc cgtcagctca ggacgctccg atttggccag
     8701 ctcttctcca acaaagctgg acaccccggc atcgtgggcg cttgccacgc tctcgatgct
     8761 ggccgagccg ccctcggcgg ccacggggtc gaaggcagtg ccgcgcacgg acagcacctt
     8821 gatggcgtcg ttggacttca ccgtcgccac agcgttgccc gcgtagatcg gccgctcgaa
     8881 ggtatccggc ccgaccacac cctggatgtc ggatatctgc gccacgtcga gcacggctgc
     8941 cacccggggc agaaagttct tgccgaccgt ggtatgggca gtgaggatgt gggagtaccc
     9001 ggcgccaatt tccgccacca gcggcgcgac gttctccgca agctgatgcc cgtaggcagc
     9061 attgtctgcc aggagcacct tgttgacccc gggaatcttg gcggcggttt cagccaccgc
     9121 gccgcagcct tcacctgcga ccaggatgtc gatatcgccg ccgatggccg tcgcggccgc
     9181 caccaccacc agggtgaccg gcttgagttc cgcgttgtcg tggtctgcaa ctaccaatac
     9241 gctcatgaga ttacctttgc ctcgttcttc agcttgtcga ccagctcctc gacgctcccc
     9301 accttgatac ccgcctgccg ttcggccggc ggtttgacgg aaagcgtctc cacggtggga
     9361 gcaacattca cccccagctc gtccggcgtg acgacgtcca gaggtttctt cttggccttc
     9421 atgatgttgg ggagagacgc gtagcgcggc tcgttcagcc gcaggtccgt ggtgaccacg
     9481 cagggcagcg tgacggagac cgtctgcaga ccgccatcca cctcccgcac gatctgcgct
     9541 ttgccatcgg ctacggtgag ctccgaggcg aacgtgccct gaggcatccc ggtcagagcg
     9601 gcgaacatct ggccggtctg ggcgttgtca ccatcgatgc tctgcttgcc gaagatcacg
     9661 atgtcggcgt tttcccgctc ctgcagcgcc ttgagcgcct tggcaatgcc aagaggctgc
     9721 ggctcctgat ccgtctcgat gagcaccgca cgatcggccc cgagcgccag gcaggttctg
     9781 agctgctcct gacaaacggt gccaccgacc gccacggcga ccacctcggt agccgttccg
     9841 gcttccttga ggcggacggc ttcttccacg gaaatctcgc agaatgggtt caacgacatc
     9901 ttgacgttgt tcaggtcgac gccagtgttg tcgggcttga cccggatctt gacgttgtag
     9961 tcgaccacgc gttttaccgg aaccagtacc ttcatgaatt cctcgaacgg ctaacaagtt
    10021 aaacagaaca gcaggtgccg acgtttcgac ggctacccgg aggggcgcgt attctccctt
    10081 gggtcctcac cgaggtcaag acgaaggggg cccgaggatc ggcagcgcag aaccggtgcg
    10141 cgggtgaagg ccctccccta taatatttga gctaatattt gagcccagca gcccgcccgg
    10201 tcggggcctt tgaagctgcg acgcaaaccc gcaaatcaaa gcagtggaga gtcatcgtgg
    10261 aaagagagtc catggatttc gacgtcgtca tcgtgggcgg tggtccctcc ggcctggcag
    10321 ctgcctgccg gctgatgcag ctggccgaat catcgggtca ggagctgagc gtctgcctgg
    10381 tggagaaagg ctcggagatc ggggcgcaca ttctttccgg cgccgttttc gagcccaccg
    10441 cgttgaacga gttgtttccc gactgggagg agcgtggtgc gccgctggac aacccggtga
    10501 cccttgacga ggtctactat ctcgtcaacg aggaaaaccg cctgaagatg ccgggcttcg
    10561 ccgtgcccaa agtcacccac aatcacggca accacgcgat cagtctgggc aacctctgcc
    10621 gctggctcgg ggagcaggcc gagaacatgg gcgtgaacgt ctttcccgga tttgccgccg
    10681 ccgagatcct ctatggggaa gacggggccg tggccggcgt cgccacgggc gacatgggcg
    10741 tcggcgcgga cggcgaacac aaggcaactt acgcgcccgg atacgagctc aggggcaagt
    10801 acaccatctt cgccgagggc tgccgcggcc atctcggcaa gcagctgatg gccaagttcg
    10861 atctgcagcg ggattcggcc acccagcact acgccatcgg cttcaaggaa ctatgggacg
    10921 tgacccccga gaatcacgtg ccgggcaagg tggtgcacac catgggctgg cctggcaggc
    10981 acttgaaagg catgttcacc gggtcctacc tctaccacct gcccaaccag caggtgtctc
    11041 tcgggctgat cgtggacctg tcctacagca atccccacgt gagcccgttc gacgagttcc
    11101 agcgctggaa gcaccatccg ctcatcgccg agaccctcag cgggggcaag cgcgtctcct
    11161 acggtgcccg ggccatcgtc aagggcggcc tgtccgcgct gcccaagcta tcggtgcccg
    11221 gcgcgctgct ggtgggtgac gatgccggct ttctcaacat gaccaagatc aagggcagcc
    11281 acaccgccat gaaatcgggc atgctctcgg ccgaagccgt cttcgagtcc ctttccggcg
    11341 gttccgcggg cggcgaggac ctgtccagct tccaggagaa gttcgactcg tcctggctgc
    11401 acgaggagct ttcagcggcg cggaattccc agcaggccat ccacaagttc ggcaccctcg
    11461 ccggggccgc gttcatctgg gtggaccaga ccctgtttcg cggcaggctg ccgttcactc
    11521 tgaaggatcc cacgcccgac cacgccacgc ttaagccggc cgcccagagc aggcggatcg
    11581 actaccccaa gcccgacggc gtgctttcct tcgacaagct gtcctccgtc tttctttcga
    11641 acaccaatca cgaggaagac cagccctgcc acctggtgct ggccgacccg accatcccca
    11701 tcagccagaa cctgccgacc tacgacgagc cggcccagcg ttactgccct gcaggcgtct
    11761 acgaggtggt ggaggaaaac ggcggcgcca gattccagat caacgcgcag aactgcgtgc
    11821 actgcaagac gtgcgacatc aaggacccgg cccagaacat caactgggtg gtccccgaag
    11881 gcaccggcgg gcccaactac ccgaatatgt agggaactcg ccggtctgtc cggtcctggt
    11941 cagaaacgaa ggccggctag gagacgctgc ccaggacgaa ccgctcgccg cggctgtaca
    12001 gacagagctc gccggcttcc tccacggcaa gctccaccag ccggtagaag accggccggg
    12061 caatgagcgc ttcgagcccg tcacgcacca tcacgtaggg ccttggcacg cccccgtggt
    12121 cctccacgcg cacgggatgt gcgctgtcgg caacgacggc atcgcccgta ttgatgacga
    12181 agaccaggtc ctgcccgccg ctctcccctc gagcttcgaa atccacggcc acgaacggcg
    12241 cgtcctcgac cgtgatcagg agcttttccg ccggggtcac caggcaatag ccgtcggagt
    12301 ccctgcgcag cacggtgctg aaaagatcca ccatgttctt gcgccggatg ggctcgcctt
    12361 cgtgatacca ggtcccgtcg gaggcaattc ggatgtcgat gtggcccacc cgggcaggat
    12421 tccagctcgc caccggcggc agccggcggt cccgctgcgc cgagacgatg gcttcgaaga
    12481 tgctgctggt gtccgtcatg tcggaaagaa cggcttgctt tgatggggcg ccgaccggca
    12541 gatcggtctt acccgctcac ccgccgatga atttcatcac ggtgacctca ccggtgaagc
    12601 cgaggtcctg cttgtcggcc aatgcctcca cgagcacgcc ctgcagctgc gcgatggcct
    12661 gatgacgggg cagcgccagc aggggcttca cgtcgtgccg ggcggtgttg gagaggcacc
    12721 cgtagatgaa gccgttggac gacagccgca ggcgggtaca ggagctgcag aacggctcgg
    12781 tctcgttggc gatgatcccg aagaaacccc gatcgggtat ctcgtagcgc acggccgtgg
    12841 agtcgtaagg ggcgtcggtt cgggtgaact cgtagcgctt gccaatggtc tcgagtatct
    12901 cctccatccc cacgaaatcc cgcaggtact gattgccctg gcgaagatga cccatgttca
    12961 tgagctcgat gtagcgcagc tcgaacccac gctccagaca gaactccagc ataggcacca
    13021 actgatcgag attggccgtg cgcattggga ccatgttgat cttgaccttg agcccaccct
    13081 cggcaagcgc tccgatgccg ccgagcacgg tctcgaggtc gccgccccgg gcaatgtccc
    13141 gaaaccggag agggtcgagc gtgtcgaggc tgacgttgat ccgcttgagg cccgattcga
    13201 gcagcaggtc gatcttgcga ggcacgagct ggccgttggt ggtgatcgag acgtcgttca
    13261 gcggcagagc catgagccgg ggcagcacct cgtcgaactt gggcgagagc agcggttctc
    13321 caccggtgat gcgcagctgc tcgatgccgg ccgcatcgac cacgagctcg accgcgtacg
    13381 cgagctcctc gccgtccagc tcgtgctggg ccgactgcag gcgttttccg tccggaacgc
    13441 agtagctgca ggcgtagttg catgcggcgg tgaggctgac cctcaaattg cggaagcgac
    13501 gccccagcgc gtctacgatc atctgctggc cccgcggacc ccgtggattg gggcggagta
    13561 tgacacggcg ccatgctcag gcaaatgtgc tagcgggcgc gcgtaaatga gaaccagttc
    13621 cggaaatctg agcagccgat tggtttactt gttcccggct gctgccatgt ctcccccccc
    13681 caacgaccag ggcagcggct gtggaacggc cttgccgttc cagtatgtgt gacctacgga
    13741 cctttatggt gcgtacccgg cgtcctcccc aggcgccggg ttttttttgc cttcaacgat
    13801 cgtccggctt ctgggggaat acgcagcgca tgacgttctc cgtcggcagg ctggtttgcg
    13861 cgcgggtgcc gtccaggcgt ttgaccttca gctcagagcc ttcccagtcg ttgtcctcga
    13921 cgatgaggta acaatggccc tcgtaattga aagtccggcc gttgagatgc gtattcgcca
    13981 tcgtcgatcc ccttggttac tgctgcgccc atgatggcaa agcagttccg gccagttgtg
    14041 tgaggtttaa tccgacattg taaacgtgtg cagccgttcg ctgaaacgat tgacgagccc
    14101 cttagcccag aaaatgcgat tggagccctt gaagagtacc cggtcgccgg atctgaggtc
    14161 ctcgaggatc tcatcgatga gcccttcacc gccggcgtcg gggagccacg agccacgctg
    14221 accctcgggc agcgcatcga agagctcgcg catggcctct cccacgcagt agacgcgatc
    14281 aattccgctg caggcttcag cgagcccccg gtgatgggcc ggcccctcgg cacccaactc
    14341 gagcatgtcg ccaagcacgg cgaccttacg accgggtacg gcggcaagcg tacgcagagc
    14401 cgccttcatg ctctcggggt tggcgttgta gctgtcgtcg accaccgtca cgccgttgat
    14461 gagcgtttcg ttgccacggc ccttcggcac caggtcgtca cccagctgca ggccatcgcg
    14521 caccgggcga tccagaactt tcagagtggc cagcacggcg gcaagggacg ccgcgcgatg
    14581 ctcgccaccg ccgggcacgt gagcggtgaa gggctcgcct ccgatcaggt agtcggccgt
    14641 ctgtccgcgc acc
//
LOCUS       DBNHLM010000260         3932 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_516242, whole genome shotgun sequence.
ACCESSION   DBNHLM010000260 DBNHLM010000000
VERSION     DBNHLM010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_516242"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="AC16QF_12185"
     CDS             complement(<1..572)
                     /locus_tag="AC16QF_12185"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6449363.1"
                     /translation="MTSAARIAGLPSYFIVFVASMVAIGPFAIDTYLPAMPAMATNLG
                     VEIVSVNATLSTYLFGFAFGQMFGGPISDQIGRRRIGLLGLVVFSAASVLIALAESIE
                     VILWLRALQAVGGGFATVICMAMVRDAYEPMEAAKRFPVVMLVMLGAPLAAPAIGAAL
                     LSFGWPSIFIFLALYGLAVLVAFLPVPETAS"
     gene            complement(591..2222)
                     /locus_tag="AC16QF_12190"
     CDS             complement(591..2222)
                     /locus_tag="AC16QF_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6449364.1"
                     /translation="MGETVAEETVAADADAVVIGAGFAGLYMLHKLRQCGFSVRGFEA
                     GDDVGGTWYWNRYPGARCDVESLDYQFAFSDELQRGWTWSERYPTQAEILRYLGYVAD
                     TLDLRRDIRFETRVSAARFDEAHNLWRVTTDRGEELSARYVIAAVGCLSVAQIPDIPG
                     LQDFEGETYHTGNWPHQPVDFTGRTVGIIGTGSSGVQAIPVIAAQAKALVVFQRSPNF
                     SVPARNAPLPADRAERYKDEFLAAREAACRTDFGNTTWRSLPSALEADPDERAAEYER
                     RWQEGGAGFMVTYSDLMSDRAANDTAAEFLRGKIRAIVRDPETAELLSPRDHPIGAKR
                     ICVDTDYYETFNRENVKLVDVRSHPIERITASGISTTAGDYDLDCLIFATGFDAMTGP
                     LTSMDIRGRDGVSLRAAWQDGPRCYLGLGVAGFPNLFTITGPGSPSVLSNMVLSIEQH
                     VEWIADCLVKLRKDGAEAIEALPDAQDGWIEHVNEAADATLFPQGNSWYIGANVPGKP
                     RVFMPYTGGVRAYREKCEEVVGNSYEGFDIRGGAS"
     gene            complement(2235..3512)
                     /locus_tag="AC16QF_12195"
     CDS             complement(2235..3512)
                     /locus_tag="AC16QF_12195"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6449365.1"
                     /translation="MSEATESIETCPIHAIRPMLPENLAYPVAMNRRLRKDAPVYQDP
                     DSGIWFVSRYEDVINMSMDHKTFSSVMPRLSTGRADSAVDPEVAAAMAEGYPIPDTML
                     TQDPPLQRRYRKFVDGAFSPRSLKALEPFIEQTSNELIDAFIDQGHCEFLAEFGVQLP
                     LRVIASQIGCPMEDLPLLRKWTEAFIANLSQQLDQPGRVQAAKDMVECQHYFVERIEE
                     RRRAPTDDILSKLVNARFDDDKPLDNPECLSMLQQILVAGNETTSSSLTEGMWLLIRN
                     PDQYEMIRRDPSPEMISKLVEEVLRISSPSANMFRRTTRDVEVSGVTIPANSICFARF
                     AAANQDDEQFPDPLKFDITRPNLKEHVAFGKGVHHCLGAALSRREMNGGFKVIFERME
                     NFRLTPDTPEPVFMPNALLHGLARLDLSFDKIR"
     gene            3688..>3932
                     /locus_tag="AC16QF_12200"
     CDS             3688..>3932
                     /locus_tag="AC16QF_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449366.1"
                     /translation="MKPRLRLLLAACCVALLAGCDNDDDDDIVAPPAPEPVQTEPVNG
                     SGVKGPLAGAVVELYAIDLAQANIQGELIDDGSTGPDA"
BASE COUNT          675 a         1252 c         1342 g          663 t
ORIGIN      
        1 gaagcggttt ccggcaccgg caggaaggcg acgagcacgg ccaggccgta cagggcgagg
       61 aagatgaaga tgctcggcca gccgaagctc agcagcgcag cgccgatcgc cggcgccgcc
      121 aggggggcgc cgagcatgac aagcatcacc acgggaaagc gcttggccgc ttccatgggc
      181 tcgtaggcgt cgcgcaccat ggccatgcag atcaccgtgg cgaagccgcc gccaacggcc
      241 tgcaacgccc gcagccagag gatgacttct atggattcgg ccaaggcgat gagcacggac
      301 gcggcagaga agacgactag gcccagcagc ccgatgcggc gccggccgat ctgatcggag
      361 atcgggccgc cgaacatctg cccgaaagca aagccgaaga ggtacgtgct gagcgtggcg
      421 ttgaccgaga cgatctccac gccgaggttc gtcgccatcg ccggcatggc gggcaggtag
      481 gtatcgatgg caaagggccc gatcgccacc atggacgcga cgaacacgat gaagtagctg
      541 gggagacccg cgatccgggc agcgctcgtc acgtcagttg ccggccgggc tcagctcgcg
      601 ccgccgcgaa tgtcgaagcc ttcgtaactg ttgccgacca cctcttcgca cttctcgcga
      661 tacgcgcgca cgccgccggt ataaggcatg aagaccctgg gtttgcccgg gacgttggcg
      721 ccgatgtacc aggagttgcc ctgagggaac agcgtggcgt cggcggcctc gttgacgtgc
      781 tcgatccagc cgtcctgggc gtcggggaga gcctcgatgg cctccgcgcc gtccttacgc
      841 agcttcacca ggcagtcggc aatccactcc acgtgctgct cgatggatag caccatgttg
      901 ctcagcacgg aagggctgcc cggcccggtg atggtgaaga ggttggggaa tcccgcaacg
      961 cccaggccca gatagcagcg cggcccgtcc tgccaggccg ccctcaggct gacgccatcg
     1021 cgaccgcgga tgtccatgga cgtgagcggg cccgtcatgg catcgaaacc ggtagcgaag
     1081 atcaggcagt cgagatcgta gtcgccggcc gtcgtgctga tcccgctcgc cgtgatgcgc
     1141 tcgatgggat gagaacgtac gtccacgagc ttcacgttct cgcggttgaa ggtttcgtag
     1201 tagtcggtgt ccacgcagat gcgcttggcc ccgatggggt ggtcgcgggg cgagagcagc
     1261 tcggccgtct cagggtcgcg gacgatggcg cggatctttc ctctgagaaa ttcggccgcg
     1321 gtatcgttgg ccgcccggtc ggacatgagg tcgctgtagg tgaccatgaa gccggcaccg
     1381 ccttcctgcc agcggcgctc gtactccgcc gcgcgctcgt ccggatcggc ttccagggcc
     1441 gacggcaggc ttctccacgt ggtattgccg aagtcggtcc ggcaggccgc ttcgcgcgcg
     1501 gccagaaact cgtccttgta gcgctcggcc cgatcggccg gcaggggagc gttgcgggcc
     1561 ggcacgctga agttgggcga gcgctgaaat accacgagcg cctttgcctg ggcggcgatc
     1621 acgggtatgg cctgcacgcc cgaggagccc gtgccgatga tgccgacggt gcgcccggtg
     1681 aaatccaccg gctggtgggg ccagttaccc gtgtgatagg tctcgccctc gaagtcctgc
     1741 agcccgggga tgtcggggat ctgggccacg gagaggcagc ccacggcggc gatcacgtag
     1801 cgggcgctga gctcctcgcc ccggtccgtg gtcacccgcc aaaggttgtg cgcctcgtcg
     1861 aagcgcgcgg cgctcacccg ggtctcgaac cggatatccc gacgcagatc gagggtgtcc
     1921 gccacgtagc cgaggtagcg caggatctcg gcctgggtgg ggtagcgctc ggaccacgtc
     1981 cagccgcgct gcagctcgtc ggagaaggcg aactggtagt ccaggctctc cacgtcgcag
     2041 cgcgcgcccg ggtagcggtt ccagtaccag gtgccgccca cgtcgtcgcc tgcctcgaag
     2101 ccgcgtaccg agaagccgca ctgccgcagc ttgtgcagca tgtagaggcc ggcgaagccc
     2161 gcaccgatga cgacggcatc ggcgtcagca gcgacggtct cctctgcaac ggtctccccc
     2221 acgcccgctt gcgatcaacg gatcttgtcg aaggaaagat ccagccgcgc cagcccgtgc
     2281 agcaaggcgt tcggcatgaa caccggctcc ggcgtgtccg gcgtcaggcg gaagttctcc
     2341 atgcgctcga agatgacctt gaacccaccg ttcatctcac ggcgcgacag cgccgcgccg
     2401 agacagtggt gcacgccctt gccgaacgct acgtgctcct tcaggttggg ccgggtgatg
     2461 tcgaacttca gcggatccgg aaactgctcg tcgtcctggt tggccgccgc aaagcgggcg
     2521 aagcagatgg agttggcggg gatggtcacg ccgctcactt ccacgtcccg cgtggtgcgg
     2581 cggaacatgt tggccgacgg gctggagatg cgcagcacct cctccaccag cttgctgatc
     2641 atctccggcg acgggtcccg gcggatcatc tcgtactggt cgggattgcg gatcagcagc
     2701 cacatgcctt cggtgaggga cgacgaggtg gtctcgttgc cggccaccag aatctgctgc
     2761 agcatcgaca ggcattccgg gttatccagc ggcttgtcgt catcgaagcg ggcgttgacg
     2821 agcttggaga ggatgtcgtc cgtcggcgcg cggcggcgct cctcgatgcg ctccacgaag
     2881 tagtgctggc actccaccat gtccttcgcc gcctgcaccc ggcccggctg gtcgagctgc
     2941 tgggagagat tcgcgatgaa ggcttccgtc cacttcctga gcagcggcag gtcctccata
     3001 gggcagccga tctgggaggc gatgacccgc agcggcagct gcacgccgaa ctcggccagg
     3061 aactcgcagt ggccctggtc gatgaaggcg tcgatgagct cgttggacgt ctgctcgatg
     3121 aagggctcca gggccttgag gctccggggc gagaacgcgc cgtccacgaa cttgcggtag
     3181 cggcgctgca ggggcggatc ctgggtcagc atggtgtccg ggatcgggta gccttcggcc
     3241 atcgccgccg ccacctccgg gtcgacggcg ctgtccgccc gaccggtcga caggcgcggc
     3301 atgacgctgg agaacgtctt gtgatccatg gacatgttga tcacgtcctc gtatcgggat
     3361 acgaaccaga tgccgctgtc cgggtcctga tagacgggtg cgtccttgcg cagccgccgg
     3421 ttcatggcca ccgggtaggc caggttctcc ggcagcatgg gccggatggc gtggatcggg
     3481 caggtttcga tggattctgt tgcttcgctc atgggtcttt gtctctgaat ggcaggccga
     3541 tgcgcccctc agcgctgcgt cggggagcgg ggaaactagc gcaccttgcc tttcgttgcc
     3601 agcgtattgc ccgcaggatc taaatgaggt aaaaaaagtt tctgatcaac aagattgact
     3661 aataactggc aaataggggg agatcgcttg aagccaagac tccgattact gctggcggcc
     3721 tgttgcgttg cgctgctcgc cggatgtgac aacgacgacg atgacgatat cgtcgcgccc
     3781 ccagcaccag agcccgttca aaccgaaccc gtcaacggtt ccggcgtgaa ggggcctctg
     3841 gccggcgcgg tggtggagct ctacgccatc gaccttgccc aggcgaacat ccagggcgag
     3901 ctgatcgacg acggctccac gggtcccgac gc
//
LOCUS       DBNHLM010000261         3636 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1115501, whole genome shotgun sequence.
ACCESSION   DBNHLM010000261 DBNHLM010000000
VERSION     DBNHLM010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1115501"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="AC16QF_12205"
     CDS             <1..218
                     /locus_tag="AC16QF_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449367.1"
                     /translation="THQALMQGYLTHLAEHRYPEARAAMTRWLKSGELKNVEYRLQGI
                     ENVGRAFCDLFGGNNFGKTIVRLRDEQ"
     gene            256..492
                     /locus_tag="AC16QF_12210"
     CDS             256..492
                     /locus_tag="AC16QF_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449368.1"
                     /translation="MPILKHPLSGAIYTARDDGLVEVDNNGLKGLFHFDGRHHSGELR
                     HADPHMLLWLAGPQLPADAAVRINRPAPVPPSEG"
     gene            549..1886
                     /locus_tag="AC16QF_12215"
     CDS             549..1886
                     /locus_tag="AC16QF_12215"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6449369.1"
                     /translation="MAAIVDRSKGISYQELIAQDPVAPPKTLTLENAYQPGIATRVPV
                     SRYASKEFHDLEMEKLWPRVWQMACREEEIPEVGNYTIYEIGSFSIVVVRTNQGIKAH
                     HNVCRHRGRRLCDHPGHASSFICPFHGFSWNIDGSLRSVTSEWDFPHIDKDELTLTPV
                     RCETWAGWVFVNMDMNAEPLMDFLGDLPAHFAVWEPENRYIEAHVAKIMSCNWKACQE
                     AFMEAFHVINTHPQILVGIGDENTQYDVWGNFSRAITPNGTPSPHLRFEPDEQELFDS
                     VTMRYMDRPSLGEVPEGMTARAMMAAGARPAIAALVGEGKDLSDASLSDSIYYTLFPN
                     FHPWGGYNRIVYRFRPYKNHHDKSIMECYYLSPFAGERPEPAPVHWLGEDEDWTEATE
                     LGILAKVFQQDTFNLPKVQIGLEAAQYEEIVLANYQESKLRHFHTLLGEWIGR"
     gene            1913..2341
                     /locus_tag="AC16QF_12220"
     CDS             1913..2341
                     /locus_tag="AC16QF_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449370.1"
                     /translation="MNFKIEWSRRPWWMNLIWFFCLYMTFIYMPFDLFLKPVEIDEEV
                     WFGVTLKGWPAKLTAPLHWLVYGAGAYGFWKMSRWMWPWAAVYVAQVSFSMLVWNLLD
                     HRGAGWLAGLLAAAVFAVPTVALWRARSIFRDAPEAAEPI"
     gene            complement(2338..>3636)
                     /locus_tag="AC16QF_12225"
     CDS             complement(2338..>3636)
                     /locus_tag="AC16QF_12225"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6449371.1"
                     /translation="TSLIGWEWSSVPGGANLHRIVVTDADAQTAKTFQPFSSLESPYP
                     DDLWAWLEKTSAATSAAFIAIPHNSNISKGWMVPRETLRGGPVDAAYGTTRMRWEPLV
                     EATQIKGDSETHPLLSPNDEFADFEEYSFYIEQQPGEYKPSEGDYVRPALKTGLELGE
                     ETGVNPYQFGLIGSTDAHTGLSTAAEDNFWGKMATDSTPETKINTLITGGSRGWTMSA
                     SGMAAVWAEENTREAIVDAMRRREVYATTGPRLRVRFFGGWEYDQADLEVEDLAAAGY
                     AKGVPMGGELTAAPENNGPRFMVVALKEPGGANLDRIQIVKGWLNAEGEAEERVYDVV
                     WSDGRQPDADGKLTPVGNTVDLMTVRYTNDIGAVELASVWQDPDFDPGQSAFYYARVL
                     QIPTPRHAQYDARALGMDRPTIGPAVIQERAYTSPIWYAP"
BASE COUNT          637 a         1183 c         1166 g          650 t
ORIGIN      
        1 tgacccatca ggcgctcatg cagggctacc tcacgcacct cgccgagcat cgctatccgg
       61 aagcccgggc cgccatgacg aggtggctga agtcgggcga gctcaagaac gtcgagtacc
      121 ggcttcaggg catcgagaac gtcggccgcg cgttctgcga tctgttcggg gggaacaatt
      181 tcggcaagac catcgtgcgg ttgcgcgacg agcagtgatg cggtacctgt atacgcagag
      241 gaggagaaga agaccatgcc gattctgaag catcccctgt cgggagccat ttacacggcc
      301 cgggacgacg ggctcgtgga ggtggacaac aacgggctca aaggcctgtt tcacttcgac
      361 ggtcgccacc attccggcga gctgcgccac gccgatccgc acatgctgct gtggctggcc
      421 gggccccagc tgccggctga cgcggccgtg cgcatcaacc gccccgcacc ggtgccgccc
      481 tccgagggct gaaccggcgc accgggcgcg gacgacaccg aaccaaccga catatccagg
      541 agaacgaaat ggccgccatc gtcgaccgat cgaaaggcat cagctaccag gagctcatcg
      601 cgcaggatcc cgtggcgccg ccgaagaccc tgaccctcga gaacgcctac cagccgggca
      661 tcgcgacccg cgtccccgtg agccgctacg catcgaagga atttcacgat ctggagatgg
      721 agaagctgtg gccccgggtc tggcagatgg cctgccgtga ggaggagatc cccgaggtcg
      781 gcaactacac catctacgag atcggcagct tctccatcgt ggtggtccgt accaaccagg
      841 gcatcaaggc acatcacaac gtctgccgtc accggggccg acggctttgt gatcatccgg
      901 ggcacgcctc gagcttcatc tgtcccttcc acgggttcag ctggaacatc gacggatcgc
      961 tgcgatcggt cacctccgag tgggatttcc cccacataga caaggacgag ctgacactca
     1021 cgcccgtgcg ttgcgagacc tgggccggct gggtgttcgt caacatggac atgaacgccg
     1081 agccgctcat ggacttcctt ggcgatctgc ccgcgcactt cgccgtctgg gagccggaga
     1141 accggtacat cgaagcccat gtggccaaaa tcatgagctg caactggaaa gcgtgtcagg
     1201 aagccttcat ggaggccttc cacgtcatca acacccaccc gcagattctg gtggggatcg
     1261 gcgacgagaa cacccagtac gacgtctggg gcaacttctc ccgggccatc acgcccaacg
     1321 ggacgcccag cccgcacttg aggttcgagc cggacgagca ggagcttttc gactcggtga
     1381 ccatgcgtta catggatcgg ccctccctgg gggaggtccc cgagggaatg accgcccggg
     1441 ccatgatggc ggccggtgcg cgccccgcca tagcggccct cgtgggggag ggtaaggacc
     1501 tgtcggacgc ttcgctgtcg gacagcatct actacacgct cttccccaac tttcacccgt
     1561 ggggcggcta caaccggatc gtctaccgct tccggccgta caagaaccac cacgacaagt
     1621 cgatcatgga gtgctactac ctgagcccct tcgccggcga gcggccggag ccggcccccg
     1681 tgcactggct gggtgaggac gaggactgga ccgaggccac tgagctcgga atcctcgcga
     1741 aagtcttcca gcaggacacc ttcaacctgc ccaaggtgca gatcggcctg gaagccgccc
     1801 agtacgagga gatcgtcctg gccaactatc aggaaagcaa gctgcgccac ttccacacgc
     1861 tgctggggga gtggatcggg cggtagatcc gtaaccaggc gcggtgtttg tcatgaactt
     1921 caagatcgaa tggtcgcgac ggccctggtg gatgaacctg atctggttct tctgcctcta
     1981 catgaccttc atctatatgc ccttcgatct gtttctcaag cccgtggaga tcgacgagga
     2041 agtctggttc ggggtgaccc tgaaaggttg gcccgccaag ctcacggccc ccctgcactg
     2101 gctcgtatac ggcgccggcg cctacggctt ctggaagatg agccgctgga tgtggccctg
     2161 ggcggccgtt tacgtggctc aggtgagctt cagcatgctg gtgtggaacc tgctggatca
     2221 tcgcggcgca ggctggttgg ccggcctgtt ggcggcagcc gtcttcgcgg tgccaaccgt
     2281 tgctctctgg cgcgcccggt cgatttttcg tgacgcgccg gaagctgctg agccgatcta
     2341 gggcgcgtac cagatcggcg aggtgtaagc gcgctcctga atcaccgctg gccctatggt
     2401 aggccgatcc atgccgagcg cacgcgcatc gtactgtgcg tgccggggcg tagggatctg
     2461 cagcacgcga gcgtagtaga aggccgactg ccccgggtcg aaatccggat cctgccagac
     2521 ggaggcaagc tcgaccgcac cgatgtcgtt ggtgtagcgg acggtcatca gatccacggt
     2581 gttgcctacg ggcgtcagct tgccgtccgc gtcgggctgg cgaccgtcag accagaccac
     2641 gtcgtagacg cgttcctcgg cttcgccctc ggcgttcagc catcccttaa cgatctgaat
     2701 gcggtccagg ttggcgccgc ccggctcctt gagcgctacc accatgaacc gcggcccatt
     2761 gttctctggc gcagcggtca gctccccgcc catgggaacc cccttggcgt acccggcggc
     2821 cgccaggtcc tcgacctcga ggtctgcctg gtcgtactcc cagccgccga aaaaccgcac
     2881 gcgcaggcgc gggccggtgg tggcgtagac ttcccggcgc cgcatggcgt cgacgatggc
     2941 ctcgcgggtg ttctcttcgg cccagaccgc ggccatgccg gaggcggaca tggtccaacc
     3001 ccgggaaccg ccggtgatca gcgtgttgat cttggtctcc ggcgtggagt ccgttgccat
     3061 cttgccccag aagttgtcct ctgccgcggt ggaaaggccc gtgtgggcgt cggtggagcc
     3121 gatcagcccg aactggtagg ggttcacgcc ggtctcttcg ccgagctcca gccccgtctt
     3181 cagcgcgggg cgtacgtagt cgccctcact gggcttgtac tcgcccggct gctgctcgat
     3241 gtagaagctg tactcctcga agtcggcgaa ctcgtcgttg ggcgagagca gagggtgggt
     3301 ctccgagtcg cccttgatct gggtggcttc caccaagggc tcccagcgca tccgggtggt
     3361 gccgtacgcc gcgtcaaccg ggcctccgcg cagggtttcc cgggggacca tccagccttt
     3421 cgagatgttc gagttgtggg gaatggcgat gaacgccgcc gaggtcgcag cactcgtctt
     3481 ctccagccac gcccagaggt cgtccggata agggctctcc agggagctga aaggctgaaa
     3541 ggtctttgcc gtctgcgcgt cggcgtccgt caccacgata cggtggaggt tggcgccgcc
     3601 gggcaccgag ctccattccc agccgatcag gctggt
//
LOCUS       DBNHLM010000262         4365 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1293345, whole genome shotgun sequence.
ACCESSION   DBNHLM010000262 DBNHLM010000000
VERSION     DBNHLM010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1293345"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="AC16QF_12230"
     CDS             complement(<1..1373)
                     /locus_tag="AC16QF_12230"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6449372.1"
                     /translation="MSKSQPNQLLLTNARLFPGVSDEVFADGAVWIEGRTIRYAGPGC
                     DLPAVADDVPRVDLHGQFVMPGMTESHSHLSYSNNGPTELDKSPVEDAMIHAVANARL
                     MLGSGFTSAISFGSVHRIDVFLRDAIDAGRIPGPRLEASSRDVGATSSNADLHPDYFK
                     PQMEGLGMLADGPWAVRKAVRTIRKNRGNVVKLFLDGEGPGGHAPPGELTYTDEEVAA
                     AVDEAHRRGMRVACHSRSAAAVKQAVRFGIDFIGHANYLDDEALDMLKRSRERLFVGP
                     AIAWELAFLRHAAELGSPPGSQRYKFYEQEVEATKNAVRRLREAGVRVLIGGDYGLSI
                     TPHGTYAKDLQYFVELFGLSPAEALLCATRDGGAAVDPSGMLGTLEEGKLADLVIVDG
                     DPLEDITVVQDHDRITAVMKDGVVHRRLAVDNPYQASPDALQEELAARAADPERQRQA
                     AVLLESSG"
     gene            1505..2281
                     /locus_tag="AC16QF_12235"
     CDS             1505..2281
                     /locus_tag="AC16QF_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019408213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6449373.1"
                     /translation="MTPELVKTELRPDGIAVVTIDNPPVNAHSGQVLTEMAWTFDAIS
                     DQDEARVVVLTGAGKVFCAGADIKSRSGRAPEPGDHWQSSRAAREAYHSVRECVKPVI
                     GALNGPALGGGLAFAASCDMLIAAETACLGLPEIDVGLLGGGRHAQRLFGHSRLRRMM
                     LTGYRVYGPELYRLGIVEACVAPDALMDEALALAAEVAAKSPIAIRLAKHALNTIEEL
                     SLRDGYRFEQTMTGQLAKTEDSKEAMRAFVEKRKPQFKGR"
     gene            2314..3171
                     /locus_tag="AC16QF_12240"
     CDS             2314..3171
                     /locus_tag="AC16QF_12240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6449374.1"
                     /translation="MKIGAVFPHLEIGSDPAVRRDWAQTAEDLGYSHLLAYDHVLGAV
                     HAGREPALWGPYTEESAFHEPFVLFGYFAAVTRRVELVTGVIILPQRQTALVAKQAAE
                     IDILSGGRLRLGVGTGWNYVEYDSLGENFANRGKRQEEQIEVLRSLWAEPVIDYSGNY
                     HRIERAGLKPLPGRRIPIWLGGFSDVAYRRAARMGDGFIFGGSIKDGLAGLGKIRGFL
                     AEAGREQAGFGIEAMVNYGAGPDRWRADLEAWEAAGADYVSMRGMDAGLETPQDHIDA
                     LRRYWEAVS"
     gene            3254..>4365
                     /locus_tag="AC16QF_12245"
     CDS             3254..>4365
                     /locus_tag="AC16QF_12245"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449375.1"
                     /translation="MSDLEAFRLETREWLEANCPPGVRNRPGEVVWGGRRCQFPNEDA
                     KIWLERMVEKGWTVPTWPKPYGGAGLNDEEAKVLRQEMARIEANAPLFSFGIAMLAPA
                     LMEFASEEQQLEHLPPIARGDFRWCQGYSEPGAGSDLASLRCRAVRDGDHYVVNGSKI
                     WTSDADVSDWIFCLVRTDPDVPKHEGISFLLIDMETPGVSVTPIELISGASPFCQTFF
                     DDVRVPAANLIGEENKGWTIAKRLLQHERAMLGETATRRGGANRKGLVEVARDALRAP
                     DGPLPDAAVREEIAQFDMDRMCYGATFQRSADAARAGRGPGPETSMFKLYLSELNQRG
                     ADLRQRIVGADGLGWAGEGFDADELQITREWLYGRAA"
BASE COUNT          705 a         1403 c         1530 g          727 t
ORIGIN      
        1 ccgctggact ccaggagaac ggccgcctgc cgctgacgct cggggtctgc ggctcttgcc
       61 gccagctcct cctgcagcgc gtcgggtgag gcctggtaag gattgtccac ggcgaggcgg
      121 cggtgcacga cgccgtcctt catgaccgcc gtgatgcggt cgtgatcctg caccacggtg
      181 atgtcttcca gggggtcgcc gtcgacgatc accaggtcgg ctagcttgcc ttcctccagc
      241 gttccgagca tgccggaggg atcgaccgcc gcgccgccgt cccgggttgc gcacagcagc
      301 gcctccgccg gcgacaggcc aaagagctcg acgaagtact ggaggtcctt ggcgtaggtg
      361 ccgtgaggcg tgatgctcag gccgtagtcc ccgccgatga ggacccgcac gccggcttcg
      421 cgaaggcgtc ggacggcgtt cttggtcgct tccacctcct gctcgtagaa cttgtacctc
      481 tgcgaccccg ggggcgagcc gagctccgcg gcgtggcgca gaaaggccag ctcccaggcg
      541 atggcgggac caacgaacag gcgctctctg cttcgtttca gcatgtccag ggcctcgtcg
      601 tcgaggtagt tggcgtgccc gatgaaatcg atcccgaacc gcacggcctg cttcaccgcc
      661 gcggcagacc tcgaatggca ggcgacccgc atgccgcgcc ggtgggcctc gtcgacggct
      721 gccgcgactt cctcgtcggt atacgtcagc tccccgggcg gggcgtgccc gccgggcccc
      781 tcgccgtcca ggaacagctt gaccacgttg ccgcggttct tgcggatggt tcgcacggcc
      841 ttgcgcacgg cccagggccc atcggccagc atgccgaggc cctccatctg gggcttgaaa
      901 tagtccgggt gaagatcggc attgctcgac gtcgccccca cgtcccggct cgacgcctcc
      961 aggcgcggcc ccggtattct gcccgcatcg atggcatcgc ggagaaagac gtcgattcgg
     1021 tgcacggatc cgaagctgat ggccgacgtg aagcccgagc cgagcatcag acgggcattt
     1081 gccacggcgt ggatcatggc gtcctccacc ggagacttgt ccagctcggt ggggccgttg
     1141 ttggagtagg aaaggtgaga atgggactcc gtcatgccgg gcatgacgaa ctgcccgtgg
     1201 aggtcgacgc ggggcacgtc gtcggccacg gcgggcagat cgcagcccgg gccggcgtag
     1261 cggatggtgc ggccctcgat ccagacggct ccgtcggcga agacctcgtc gctcactccg
     1321 ggaaagagtc gcgcgttggt cagcagcagc tggttgggtt ggctcttgct catgtctgcc
     1381 tccgattgcc gcggtggttc cgccagaccg tgggaaaagc ttaaaccacg atgccgggcc
     1441 ttggtagcgg ctcagatcca gagcgttaca atggaccccg tcagcagtcc ttgggggagg
     1501 gttcatgaca cccgaattgg tgaagacgga attgcggccg gacggcatcg ccgtggtgac
     1561 catcgataat ccgccggtca atgctcacag cggtcaggtg ctcaccgaga tggcgtggac
     1621 gttcgatgcc atcagcgacc aggacgaagc acgggtagtt gtgctcacgg gcgcggggaa
     1681 ggtcttctgc gcgggcgcgg acatcaagtc ccgctccgga cgcgcacctg aacccggcga
     1741 tcactggcag agcagccggg cggcccggga ggcttaccac agcgtgcgcg aatgcgtgaa
     1801 gcccgtgatc ggggcgctca acgggccggc ccttggcggc ggcctggcct ttgccgcttc
     1861 ctgcgacatg ctgattgccg cggagaccgc ctgtctcggt cttcccgaga tcgacgtggg
     1921 cctgctcggc ggcggccgcc acgcgcagcg gctgttcggt cactcccggc tgcggcggat
     1981 gatgctcacc ggatatcggg tatacggtcc cgagctttac cggctcggca tcgtggaggc
     2041 ctgcgtggcc ccggatgcgc tcatggacga ggcgctcgcc ctcgcggccg aggtcgcggc
     2101 caagagcccc atcgccatcc ggcttgccaa gcacgcgctc aacaccatcg aggagctgag
     2161 ccttcgcgac ggttaccgct tcgagcagac catgaccgga cagctggcta agaccgagga
     2221 ttcgaaggaa gccatgcggg cattcgtgga gaaacgcaaa cctcagttca aaggccgctg
     2281 aaggctcggc tcgtctgata cagggagaat gaaatgaaga taggggcggt ctttccgcat
     2341 ctggagatcg gcagcgatcc ggcggtccgt cgcgactggg cccagaccgc cgaggatctc
     2401 ggctacagcc accttctggc ctacgatcac gtgctgggcg cggtgcacgc cgggcgtgag
     2461 cccgcgctgt gggggcctta cacggaagag tcggctttcc atgagccgtt cgtgctcttc
     2521 ggatattttg cggcagtgac ccgccgggtc gagctggtca ccggggtcat catcctgccc
     2581 cagcggcaga cggcgctggt ggccaagcag gcggcggaga tcgacatcct ctccggcggg
     2641 cggctcaggc tcggcgtggg caccggctgg aactacgtgg agtacgacag cctcggggag
     2701 aacttcgcca atcggggcaa gcgtcaggaa gagcagatcg aggtgctgcg aagcctctgg
     2761 gcggagccgg tgatcgatta ctcgggcaac tatcatcgca tcgagcgcgc gggcctgaag
     2821 ccgctgcccg gccgccggat acccatctgg ctgggcgggt tctcggacgt cgcctaccgc
     2881 cgcgccgcac ggatgggtga cgggttcatc ttcggcggca gcatcaagga tgggcttgcc
     2941 ggcctcggga agatccgggg cttcctcgcc gaggcgggca gggagcaggc cggcttcggc
     3001 atcgaggcga tggtcaatta cggcgcgggg cccgaccgct ggcgcgcgga cctggaggcc
     3061 tgggaggcag ccggggcgga ctacgtctcc atgcgtggca tggatgccgg actcgagacg
     3121 ccgcaggatc acatcgatgc gctcagacgt tactgggaag ccgtatcgtg agttgccgga
     3181 ggggcgattg cgtcatgacc gagccctttg gacaggctag aatgagcggt tgctgtctgg
     3241 gaaaagggat gcaatgagcg atctggaagc tttccgcctc gagacccgtg agtggctcga
     3301 ggccaattgc ccgccgggtg tccgcaaccg gcccggagag gtggtctggg gcgggcgccg
     3361 ctgccagttt ccgaacgagg atgcaaaaat ctggctcgag cgcatggtcg agaagggctg
     3421 gaccgtgccc acctggccca agccttatgg cggggccggc ctgaacgacg aggaggcgaa
     3481 agttctgcgc caggagatgg cgcgcatcga agccaacgcg ccgctgttct cgttcggcat
     3541 cgccatgctc gccccggcgc tgatggagtt cgcctctgaa gagcagcagc tcgagcacct
     3601 gccgcccatc gcccgcggcg atttccgctg gtgtcagggc tacagcgagc cgggggccgg
     3661 ttccgacctc gcgtcgcttc gctgcagggc cgtgcgggac ggcgaccact acgtggtgaa
     3721 cggctccaag atctggacct cggacgcgga cgtttccgac tggattttct gcctggtgcg
     3781 caccgatccc gacgtgccca agcacgaggg gatcagcttc ctcctgatcg acatggaaac
     3841 acccggtgtc tccgtcaccc ccatcgagct gatcagcggc gcctcgccct tctgtcagac
     3901 ctttttcgac gacgtgcggg tccccgcggc caacctcatc ggcgaggaga acaagggctg
     3961 gaccatcgcc aagcggctgc ttcagcacga gcgcgccatg cttggcgaga ccgccacgcg
     4021 ccggggcggc gccaaccgta aaggactggt ggaggtggcg cgggacgccc tgcgtgcgcc
     4081 ggatggcccg ctgcccgacg ctgccgtgcg cgaggagatt gctcagttcg acatggaccg
     4141 catgtgctac ggcgcgacgt ttcagcgcag cgccgacgcg gccagagccg gccgcggtcc
     4201 cgggccggag acctcgatgt tcaagctcta tctttccgag ctgaaccagc gcggggccga
     4261 cctgcgtcag cgcatcgtcg gcgccgatgg gctcggctgg gccggcgagg ggttcgatgc
     4321 ggacgagctg cagatcaccc gtgagtggct ctacgggcgt gcggc
//
LOCUS       DBNHLM010000263        17682 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_636810, whole genome shotgun sequence.
ACCESSION   DBNHLM010000263 DBNHLM010000000
VERSION     DBNHLM010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 17682)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17682)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17682
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_636810"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="AC16QF_12250"
     CDS             <1..897
                     /locus_tag="AC16QF_12250"
                     /inference="COORDINATES: protein motif:HMM:NF015377.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HIR6449376.1"
                     /translation="NQIDLMNFQHTQRDGEPKLYAPAIYAETYAEALQKRLPEVHVTV
                     NASEYYQVHGHVCAAHSVNGIGRAGQLAPALIQETSQGLYMHPDGTPDVLAVDHLRRV
                     FAEALRTTLDTVRRFTAPAQIIDLHGQRQTFDFDCGVKALQMVLAYYGVEEREDKLLK
                     ALAADPELGTPLARMIEFAESRGFEVKAGTGWSLDDVKHYLDRNCPVIVLVQAWADGY
                     LSLAEWRRNFDDGHYVVVVGYDRQNLYFEDPASFHRTWLKENEFLARWHDQDPVTGEK
                     LLQAGMVLLGREPVGKGLRPMY"
     gene            1199..1954
                     /locus_tag="AC16QF_12255"
     CDS             1199..1954
                     /locus_tag="AC16QF_12255"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HIR6449377.1"
                     /translation="MIDDYSDRHHRFEGCFNFRDIGGYPTTEGRTVRWGRYYRAGRQD
                     RMSEADVARARDLGIATQIDLRKPDEVREQGRGPLEEMGVAYHHIPVIPEGGSDALSR
                     LVGDTGISGRRYLGYLEFGPEPWLAMFEIFADAGRHPIVLHCTAGKDRTGVSTAFLLS
                     VLGVDRALIEADYILTNRDVGRQADFIERTVGYPEGMDRAAMVHAAGVPETAIGDFLD
                     GLEARWGGPLGYLRSIGVSEATMDDVRREFLED"
     gene            1982..2488
                     /locus_tag="AC16QF_12260"
     CDS             1982..2488
                     /locus_tag="AC16QF_12260"
                     /inference="COORDINATES: protein motif:HMM:NF025050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6449378.1"
                     /translation="MQVAYHVTDIHRAAREMAEKVGAGPFFINENITLTWGEHRGEPT
                     DFVHSSAYGQWGELMVEFFQQENDTPNTPYRDMYGPDEEGLHHTAIMVPDMEEAFEYF
                     ERNGMPVVTRCGLGRSGGVDFAFIDARKLLGHMIEIYPRSDGVVGFYRHVREASVGWS
                     GEDPLRSV"
     gene            2517..3350
                     /locus_tag="AC16QF_12265"
     CDS             2517..3350
                     /locus_tag="AC16QF_12265"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6449379.1"
                     /translation="MGKLEGKVAIVTGAGRGIGRAIALKLAAEGAHVVVNDLDEQPAG
                     AVTDEINSGTGPRALAFPGDVTLPEFGDDLVDAALDGFGGLDVLVNNAGYIWNSALLN
                     HSDEQWEAMLAVHATAPFRILRAAGRHFREQAKAAQARGERPPCRKVVNVSSISGVYG
                     AATQASYAAGKAAVIGLTKSLSKEWGPYNVTVNAVAFGYIETRLTQTIGDEVETIDVK
                     GRQHRVGLTPEAIEAYRSTSPLRRAGAPEDAANAVALFCFPESDFVTGEVLIASGGTT
                     G"
     gene            3425..5044
                     /locus_tag="AC16QF_12270"
     CDS             3425..5044
                     /locus_tag="AC16QF_12270"
                     /inference="COORDINATES: protein motif:HMM:NF013355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HIR6449380.1"
                     /translation="MTIRFLGKNVSGPFTIPSGIVATAVPIIEYLFRHVPQIGVMTTK
                     SIGLAPRTGNREPIYSQYAPGCFVNAVGLTNPGAERSAELFAALDVPEDRFLLTSIFG
                     GSVEEFVAVAKILAPVSDGLELNLSCPHAKGYGMEMGRDPELVREITAAVKAAVDVPV
                     VPKLTPNADNIGEIAQAAVAGGADALCAINTVGPGYYSAAGEPVLSNELGGMSGKGIL
                     PIGLKCVREIAGRVNVPVIGCGGISSAADVNAYRAAGAHIFGVGSALTGMTSPEITQY
                     FQTLAADLADDGNDAEARIQYRLDMGFRPYTLVTNTRVTDDICILTFDRPLDIKAGEF
                     VFLWIPGLGEKPFSALTDDPFSLVVIDLGQFTHALMDLAPGTRTYVRGPHGLPAEPPP
                     GARIVAVAGGTGLAAVYQLARDHGNADIFMGARTAERLYFIDECEAAATLHVATDDGS
                     CGHHGLVTELLAKHLETLSENELANTVFYNCGPEPMVHAAVEVQRRFCPDEQIFSAID
                     YLTKCGVGICGACTAPDGRRLCVDGPFLSAS"
     gene            complement(5148..7403)
                     /locus_tag="AC16QF_12275"
     CDS             complement(5148..7403)
                     /locus_tag="AC16QF_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6449381.1"
                     /translation="MRRAILGRALSLLVPVIWLGIGMPGPALAESDSLALEEIVVTAR
                     KREESMQEVGMALSALGAEDLKIRFDADLQTLQNASPNLIVSDIQQGPGSPAAISIRG
                     IGTTDVEKNFDPTVGVVVDGVFIGVNSGAMLKAIDLESVEILRGPQGTLFGRNSIGGV
                     INVTRARPDFDGLSGSLRAGAGNHGDLQLDAYINITATENLAFRLGGAKRESDGWFKN
                     ATLDRDAGEMDYWVISPSVLWQPTESIELYYRYDKTEQEQDANTLHNIAQPDQIFCLY
                     YGQCAQGLKTPQSGKDYTVLQNDDPPYQTYFDTETHILNVSWDINDQYSVDFVYGGFE
                     TDEEVYQDWDSTPLTLYHTDRPATWEQDSFEARLNFAGERLSYTAGAYYWESDYRIDL
                     TSYIGFGAILGVPGAGFDDAIVFPVYQTVIQSTESYAFFLEADYQFADRWTLTVGGRY
                     TDDKKESGVIDALTPELAIKGNPDDPFKASWDEFTPKVGLRFQASNDLMFYGLYSKGF
                     RAGGFNGRPGGGQYLAAATPYDPETVDNFEVGMKSEWLDGRMRLNVSAFMMDYQDKQE
                     EQSVPVAGGTGQQTLVVNASEASINGVEVDFSWLVSANFTIEGNLGLLDAEYDELEDP
                     AFDPPLDLSDKKLRRAPDMTATISPVVTVPIGPGQLIARAALRYVDDMELTFLNSPQS
                     SVGSHTVLDASLTYTVNQLSISVWGLNLTEDDSWTQAYDVGTSPAFAGLWTYSAIRPP
                     RTYGAVVSWDF"
     gene            complement(7575..8960)
                     /locus_tag="AC16QF_12280"
     CDS             complement(7575..8960)
                     /locus_tag="AC16QF_12280"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6449382.1"
                     /translation="MRGIFLALVAVCLQVPVLAVAAEIDFTRHLVRVESAAGQHPGPD
                     VGPARPFFGVAGPARVIVTARSGEPVVTVRLNGVDVLDGQALRQTTSLEIPVELAEAN
                     TIAVGLDGRDGAAASVRVKQRADVALNVISWVHFNTNVSDFNEARAFYGVLGFETMSG
                     FPDTNTLAMAHALGIQTPTAYDGSQGEEAGGYLLHGELVGPDGFWGGVIDLIEFTIPR
                     NEEPPYPGLNHLGMARALMHTGDIAAAYDQLVGAGVNFLSPPATRSDGTRFAVFTDPD
                     GTFYELVELEGPGGEASEKPVIGVGRVNVNVSDFERSEAWYRMFGYVATRKLPPTDSA
                     EVAAAMGFDAPYRIDGAVLSHEVDGSQIELVQWLSPFDPEPAYPPPVSHLGIHRMALL
                     TGDIEADVAALKAQGVKFISGITPCCSGPDSWGSIVLFYDPDGTVVELVEQPFLGTVA
                     RAVIWLRRLFE"
     gene            complement(9029..10027)
                     /locus_tag="AC16QF_12285"
     CDS             complement(9029..10027)
                     /locus_tag="AC16QF_12285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6449383.1"
                     /translation="MINKKVVLASRPEGQPKESDFKVIEEELAELTEGQVLLATEHLS
                     IDAFIRTMLDSQEGLHGTVAIDTPVMALGVARVVDSRSPDLNQGDAVFGPMGAQTHLV
                     APGGMYRKLEEGDVPPRSYLGALGMTTGLTAYAGMLKVGQVAEGDTVVVSAAAGAVGT
                     IACQLAKIAGAKVIGIAGGPAKVAYLIDEIGCDGAIDYKNDDVAEKLRALAPAGVNVF
                     FDNVGGELLDIVLDQIAQGARVVICGAISQYNHMEEVRGPRLYLRIPERNASMLGFTV
                     DRFAADFPEMEADISRWMAEGKLKMPEHVVEGIEQFPKALTMLFTGGHMGKLLVAP"
     gene            complement(10068..11387)
                     /locus_tag="AC16QF_12290"
     CDS             complement(10068..11387)
                     /locus_tag="AC16QF_12290"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006720 - isoprenoid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6449384.1"
                     /translation="MSEAVSNEAPFWLSGNFAPTFDEVTETRLEVTGTIPPELKGRYL
                     RNGANPESGVTPHWFLGNGMIHGVELNDGRANWYRNRYVRTPLLSDGDHSDGVDERAK
                     SLANTHIIGHAGRILALEEGHWPFELTPELDTVGPYTFGGRLETGMTAHPKICAETGE
                     LLFFAYAATPPFMTYHRATADGELVQSEVIEVKGATMVHDFNITRNYVIFMDLPVVWN
                     FENAASVGLPLAWSDEYGARLGVMPRNGSNADVVWYDIDPCYVYHPLNAYEDGDDIVI
                     DVCRMAHHMKIGAPEAPPMLHRWTIHQSAGTVSEAQLDERSVDFPRVPDALVGQKHRY
                     GYTAEFGGGLPAAVAFRKYDLATGTSEAHVLADGRAGGEPVFVPARGGTSEDDGYLMS
                     FVYDPADNRSELVIIDASNVTADPVARVHLPVRVPAGFHGSWVADAQ"
     gene            complement(11384..11974)
                     /locus_tag="AC16QF_12295"
     CDS             complement(11384..11974)
                     /locus_tag="AC16QF_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6449385.1"
                     /translation="MLAAARELFAERGFEDTSTQQIAKTAGVSEGILFHHFGSKRGLL
                     ERIAEDFVAAGVQAAMPDTPGQLSEEMVVRGAFDFADAHPALYRLLEQMSRELGGSSG
                     ASRSAVIVAAIEKSLRAGMAQGIVREGDAGIMAELQFALVDAAYKAWRDGGDPDRRED
                     YIREAVGCMKAMLAPSGAEERPPLADATKLSRGDAT"
     gene            complement(12101..13435)
                     /locus_tag="AC16QF_12300"
     CDS             complement(12101..13435)
                     /locus_tag="AC16QF_12300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR6449386.1"
                     /translation="MSNQRAAEFWARTRDVAGTWGLALALLLAGLIVAYQFVGPPPPK
                     RFVLATGEPSGAYYYFGERFAQVLARDGVVVELRATGGSVDNLDLVAREGGADLAFVQ
                     GGIASSAPVDNLVTMGSLYLEPLWLFLGPGVVLDDLGDLKGRRLAVGVEGSGTRAVVM
                     QLLEANGVDAANSKLLGVASAALPDALAGGEIDAAFLIGAPQSSTVGKLLRLPGVRLH
                     DMERVEAYTRRNAFLSSVVLPRGVLDLEADLPAKDTETVAVGAMLVARDDFHPALTDL
                     LLIAAAEIHGGHSILADAGTFPTPRFVDLPLSEEAERHYRHGAPFLMRYLPFWAATLV
                     DRLWIMLVPLIGLSLPLARLVPPAYRWQIRRRLLRIYTKLHALDPLRTPIADDEDVAR
                     RLEELDRLDVDSTIEAVPKGYADDLYQLRRDIDLVRRRLGAARSAGRGQQND"
     gene            complement(13446..13820)
                     /locus_tag="AC16QF_12305"
     CDS             complement(13446..13820)
                     /locus_tag="AC16QF_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449387.1"
                     /translation="MTSSIQTGAVLVVFGLAIGAASASAEVCDVDQVLQRLQSEVLGA
                     LSEEQQTRAREVLGEFCGGMISESPRRVVSTADPTEEAASKAPGELQEAEESEGTLKV
                     LGVEFQKADEDSRGHERLKKKR"
     gene            13939..15681
                     /locus_tag="AC16QF_12310"
     CDS             13939..15681
                     /locus_tag="AC16QF_12310"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6449388.1"
                     /translation="MYDHLIRNATIVDGLGGKPCTGDIAIKDGVIAAVGEVSGNARET
                     LNAEGAYATPGWVDVHTHYDGQVSWDDTIEPSFSQGVTSIVMGNCGVGFAPCRPGDEK
                     RVIELMEGVEDIPGTALYEGIEWGNWESFPEYIEYLSTKRYTMDIGTQIPHSALRNYV
                     MGERALTHEDATAEDLEAMCRLVQDGLAAGALGFSTSRTIGHRSVDGEPIPGTFAEKD
                     ELLAIARAMREAGRGVFQAVPAGVVGDIAGPERWTTEQEVELFAEVARASGRKCTFTL
                     IQNGNRPDQWRNCLNIVEQANAEGIELTPQIATRPIGIVTSLDSYHMFMRRETYLKYA
                     DLPLSSRLAELRKPEVRAAILADKDVPPDQPGVMANVFALMQMAAPAMFPIDIPIDYE
                     PEPSANMGARAAAAGMDIQAFMYDFLTAADGDQFAILLGANFADGTFSVIEEMIPHPN
                     TTIGLSDAGAHVNLIFDAVGPTYQLTHWVRDRSRGRRFPIELMVHKQTYSNARLFGLN
                     DRGSIEVGKRADINVIDLERLGLGKLHVHRDLPAGGSRILQPAHGYVGTFVAGVKTRE
                     NDRDTGARPGRVIR"
     gene            complement(15785..16735)
                     /locus_tag="AC16QF_12315"
     CDS             complement(15785..16735)
                     /locus_tag="AC16QF_12315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6449389.1"
                     /translation="MVDAYLEGLYRRGRGPLLASLAGYTRRLAALTNQRAYDLVWLEK
                     ELLPWLPAAFERMLRRPYLVDYDDAIFHRYDQHPRALVRWLLGNKIDLVMRRAQVVVV
                     GNRYLADAARRAGARELEIVPTSVDTQRYRPLAKDSGRNASGDFNVGWIGTPQTAPFL
                     KTLTPVFERLHGSGGFRFTFIGVPAGLDVGVPYEAFPWTESDEVSLLQQLDCGIMPLP
                     DLPFERGKSGYKLIQYMACGLPVVASPVGENQHIVREGVNGFLASQTDEWIAALQGLR
                     DAADRGRALGMAGREIVEATYDCRRNAKRIAALLRAAVAQ"
     gene            complement(16852..>17682)
                     /locus_tag="AC16QF_12320"
     CDS             complement(16852..>17682)
                     /locus_tag="AC16QF_12320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6449390.1"
                     /translation="AHCANVIGQVIIVDNASTDSSLDFPMPTSLEVDLIRSGCNLGFA
                     AACNLGASRAGADFLLFANPDLVIGQDILTQPLRRLRGDATIGAVGIGLYDAGSGQRQ
                     RTCCRLPTPISMLARSLGIDRLWPRLGYRLSGWDHSSSRKVDHVIGACYLTPRAVFDR
                     LGGFDERFFLYLEDLDFSARIQQAGMDILYLANPCASHVGGGSSRQIKDERLFYMLRS
                     RLQYVHKHMGVWGLIIVGFSTLAIEPWLRILASVLAGSVTGCGEVLRGYGRLYRWLGS
                     "
BASE COUNT         3218 a         5886 c         5585 g         2993 t
ORIGIN      
        1 aatcagatcg atctcatgaa ctttcagcat acgcagcgcg acggcgagcc gaagctgtac
       61 gcgccggcga tctacgccga gacatacgcc gaggccctgc agaaacggct gcccgaggtc
      121 cacgtcaccg tcaacgcctc ggagtactat caggttcacg gtcacgtctg tgccgcccac
      181 tcggtcaacg gtatcggcag agccgggcag ctggcgcccg cgctgattca ggaaacttct
      241 cagggccttt acatgcaccc cgacgggacg ccggacgtgc tggcggtaga tcacctgcgc
      301 cgggtctttg ccgaggccct gcgcacaacg ctggacaccg tccgacggtt cacagcgccg
      361 gcgcagatca tcgatctgca tggccaacgc cagacgttcg atttcgactg cggggtgaaa
      421 gcgctgcaga tggtgctggc ctactacggc gtcgaggagc gcgaagacaa actgctcaaa
      481 gcgctcgccg ccgaccccga actcggcacg ccgctggcca ggatgatcga gttcgctgag
      541 tccaggggct tcgaggtcaa ggccggaacg ggctggtcgc tggatgacgt gaagcactat
      601 ctcgaccgca actgcccggt catcgtcctg gtccaagcct gggccgacgg ctacctctcg
      661 ctggccgaat ggcgacgcaa cttcgacgac gggcactacg tcgtggtggt gggctacgac
      721 cgtcagaatc tctacttcga ggaccccgcc tcgtttcacc gcacgtggct gaaggaaaac
      781 gaattcctcg cccgctggca cgaccaggat ccggttaccg gggaaaagct cctccaggcc
      841 ggcatggtgc tgctgggcag ggagccggtg ggcaagggcc tgcggcccat gtactgacca
      901 gaccgctttg gctcatcacc gaaggcagcc gcgccaggca tctccaccgc aggcgcccgg
      961 atcgacaagc acgagcacgc ccacgactac tttttcctgt tgaacgttgc cggcattcgg
     1021 cgaccggtgg cacgttcttc gtcgacgttc cagacgccgg gaccatcaac tttttcacca
     1081 ggccgctgtc tcactgacga caacgtcggg ccgggtcggt cagagacggt ctgatcgaga
     1141 cggtcgtccc gacacggtcc gtttgacatg gacgcgcttt ccgaatgaac ttcaacatat
     1201 gatcgatgac tattccgacc ggcatcaccg attcgaaggc tgcttcaact ttcgcgacat
     1261 cggcggctac ccgacgaccg agggtcgcac ggtgcgatgg ggccgctact atcgggcggg
     1321 tcgccaggac cgcatgtccg aagccgatgt tgccagggct cgcgatctgg gcatcgccac
     1381 gcagatcgac ctgcgcaagc ccgacgaggt ccgagagcag ggccggggtc ctttggagga
     1441 gatgggcgtt gcctaccacc acatccccgt catcccggag ggcggcagcg atgcattgag
     1501 ccgcctggtg ggggacaccg gcatctccgg acgtcgctac ctcggctatc tggagttcgg
     1561 gcccgagccg tggcttgcga tgttcgagat attcgccgac gccggccgtc accccatcgt
     1621 gctgcactgc actgcaggca aggaccgcac gggggtctcc accgcctttc tcctatccgt
     1681 gctgggcgtg gaccgcgctc tgatcgaggc cgactacatt ctcaccaacc gcgacgtggg
     1741 ccggcaggcg gatttcatcg agcgcaccgt cggctacccg gagggcatgg atcgggcagc
     1801 gatggtgcac gccgcagggg tgcccgagac tgccatcggc gacttcctgg acggcctcga
     1861 ggcgcgctgg ggcggaccgc tggggtacct tcgcagcatc ggcgtgagcg aggccaccat
     1921 ggacgacgtg cgccgggagt tccttgagga ctgaggagga aggaccatca aactcgacat
     1981 cgtgcaggtt gcctatcacg tcaccgacat ccaccgggcg gcccgggaga tggctgagaa
     2041 ggtgggggcc gggccgtttt tcatcaacga gaacatcacg ctgacctggg gcgagcatcg
     2101 cggcgagccc acggatttcg tgcactcgtc ggcctacggg cagtggggcg agctcatggt
     2161 tgagttcttc cagcaggaaa acgacacgcc gaacaccccc taccgggaca tgtacgggcc
     2221 cgacgaggag ggcctccacc acacggcgat catggttccc gacatggagg aggccttcga
     2281 gtacttcgag cgcaacggca tgcccgtggt cacccgctgc ggattggggc gcagcggcgg
     2341 cgtggacttc gccttcatcg acgcgcgcaa actgctcggc cacatgatcg agatctaccc
     2401 gcgttccgac ggcgtggtcg gtttctaccg ccacgttcgg gaagcctccg tgggctggtc
     2461 cggcgaggat ccgctgcgca gcgtctgagc gaaccgccat ggaggccgtt ccaatcatgg
     2521 gaaaactcga ggggaaagtc gccatcgtca ccggtgcggg ccgcggcatc ggccgcgcca
     2581 tcgcgttgaa gctggcggca gaaggtgccc acgtcgtggt caatgatctg gacgagcagc
     2641 cggccggcgc cgtcaccgac gagatcaact cgggcactgg ccctcgtgcg ctggcctttc
     2701 ccggggacgt gaccctgccc gagttcggcg acgatctcgt cgatgccgct ctggacggct
     2761 tcggcggcct ggacgtcttg gtcaacaacg ccggctacat ctggaactcc gcgctgctca
     2821 accacagcga cgagcagtgg gaggccatgc ttgccgtgca cgccaccgcg ccgttccgca
     2881 ttctgcgggc cgcgggccgc cacttccgcg agcaggccaa ggcggcccag gcccgcggcg
     2941 aaagaccgcc ctgtcgcaag gtggtgaacg tctcctcgat atcgggggtg tacggagccg
     3001 ccacccaggc gagctacgct gccggcaagg cggccgtgat cggcctgacc aagtccttga
     3061 gcaaggagtg gggtccctac aacgtgaccg tgaacgccgt ggctttcggg tacatcgaga
     3121 cacgtctcac ccagacgatc ggcgacgagg tggaaaccat cgatgtgaag ggccgccagc
     3181 accgggtggg cctcacgccg gaagccatcg aggcctaccg gagcacgagc ccgctgcgcc
     3241 gggccggagc tccggaggac gcagcgaacg ccgtcgccct gttctgcttt ccggagtccg
     3301 atttcgtcac cggcgaggtg ctgatcgcca gcggaggcac tacgggttga cgcgtggttc
     3361 gattgggcgc gccgaacctg tagaatcccg ctccgaatcc cgacaccgct gagacccgcc
     3421 cgccttgacg atccgattcc tgggcaagaa cgtatccggc ccgttcacca tcccatcggg
     3481 gatcgtcgcg accgccgtgc cgatcatcga gtacctgttc aggcacgttc cgcagatcgg
     3541 tgtgatgacc accaagagca tcgggcttgc accccgcacc ggcaatcgcg agcccatcta
     3601 cagccagtac gcgcccggct gcttcgtcaa cgccgtgggc ctgaccaacc cgggcgccga
     3661 acgttcggcc gagctcttcg ccgcgctcga cgtcccggaa gatcgctttc tgctcacctc
     3721 catcttcggc ggcagcgtcg aggagttcgt ggcggtggcg aagatcctgg cgccggtctc
     3781 cgacggcctg gagctgaacc tgtcctgccc ccacgccaag gggtacggca tggagatggg
     3841 ccgggatccg gagctggtgc gcgagatcac cgcggcggtg aaagcagccg tggacgtgcc
     3901 cgtggtgccg aagctcacgc cgaacgcgga caacatcggc gagattgcgc aagccgccgt
     3961 ggccggcggg gcggacgccc tgtgcgccat caacaccgtg gggcccggct actacagcgc
     4021 cgcaggcgag cctgtgctga gcaacgagct cggcggcatg tcgggcaaag gcatcctgcc
     4081 catcggcctg aagtgcgttc gggagatcgc cggaagagtc aacgtccccg tgatcggctg
     4141 cggtgggatc agcagcgcgg ccgacgttaa cgcctaccgc gcggcagggg cccacatatt
     4201 cggggtgggc tcggcgctga ccggcatgac gtcgccagag atcacccaat acttccagac
     4261 gctggcggca gacctggcag acgacgggaa cgacgcggaa gccaggatcc agtaccggct
     4321 cgacatgggc ttccgcccgt acacgctggt gacgaacacc cgcgtcaccg acgacatctg
     4381 cattctcacc ttcgaccggc cgctggacat caaagcgggt gagttcgtct tcctgtggat
     4441 acccggtctg ggcgaaaagc ctttctcggc gctgaccgac gatccgttct cgctggtggt
     4501 catcgacctg gggcagttca cccacgcgct catggatctc gctccgggta ccaggaccta
     4561 cgtgcgcggt ccccacggcc tgccggcgga accgccccca ggcgcgcgca tcgtcgcggt
     4621 cgcggggggc accggcctcg ccgccgtcta ccagctggca agagaccacg gcaacgccga
     4681 tatcttcatg ggtgcgcgaa cggccgaacg gctctacttc atcgacgagt gcgaagcggc
     4741 ggccaccctg cacgtggcca ccgacgacgg cagctgcggg caccatggcc tcgtgaccga
     4801 gctgctggcc aaacacctgg aaacgctgtc ggaaaacgag ctcgccaaca ccgtcttcta
     4861 caactgcggc ccggagccca tggttcacgc ggcggtcgag gtccagcgcc ggttctgccc
     4921 ggacgagcag atcttcagcg ccatcgacta cctcaccaaa tgcggggtcg gcatctgcgg
     4981 cgcctgcacc gcacccgacg gccggcgcct gtgcgtggac ggcccgttcc tgagcgcgag
     5041 ctgacgcaaa gcccactgcg cgttcctccc cagaggccga tcagcgccta cccttgggca
     5101 gaaaaaagcc ctccgaagag ggcttttcgt gccggcactg ccgaaagcta gaagtcccag
     5161 ctgaccaccg cgccgtaggt gcggggcggg cggatggccg agtaagtcca cagaccggca
     5221 aaggcggggc tggtgcccac gtcgtaggcc tgggtccagg agtcgtcctc ggtcaggttc
     5281 agcccccaga cgctgatgct gagctggttc accgtgtagg tcagcgacgc atccagcacc
     5341 gtgtgggagc ccaccgagct ctggggcgag ttcaggaacg tcagctccat gtcgtccacg
     5401 tagcgcaggg cggcccgggc aatgagctgg ccgggcccga tgggaacggt caccaccggc
     5461 gagatggtgg ccgtcatgtc cggcgctcgg cgcagcttct tgtcggaaag gtcgagggga
     5521 gggtcgaacg ccggatcctc gagctcgtcg tactccgcgt cgagcagccc caggtttcct
     5581 tcgatagtga agttcgcact gacgagccag ctgaaatcga cttccacccc gttgatggat
     5641 gcttccgagg cgttgacgac cagggtctgc tggccggtgc ccccggcgac aggcacgctt
     5701 tgctcttcct gcttgtcctg gtagtccatc atgaaagcgg agacgttcag acgcatgcgg
     5761 ccgtcgagcc actcgctctt catgcccacc tcgaagttgt ccacggtttc tggatcgtaa
     5821 ggggtagccg cggccagata ctgaccgcca ccgggacggc cgttgaagcc ccctgcccgg
     5881 aagcccttgg agtagaggcc gtagaacatg aggtcgttgc tggcctggaa gcgcaggccg
     5941 accttgggcg taaattcatc ccaggaagcc ttgaacggat catccggatt gcccttgatg
     6001 gcgagttccg gcgtcagcgc atcgatcacg ccgctctctt tcttgtcgtc cgtgtagcgt
     6061 cctccgaccg tcagagtcca gcgatctgca aactggtagt cggcctccag gaagaacgca
     6121 taggattccg tcgactgaat gaccgtctgg tacacgggga agacgatggc gtcatcgaag
     6181 cccgcacccg gcacgccgag aatcgctcca aagccgatgt agctggtcag atcgatacgg
     6241 tagtcggact cccagtagta ggcgcccgcc gtgtagctca gccgctcgcc ggcgaagttg
     6301 agcctggcct cgaagctgtc ctgctcccag gtggcgggcc ggtcggtgtg gtagagggtc
     6361 aggggggtcg aatcccagtc ctgatagact tcttcgtcag tctcgaaacc gccgtagacg
     6421 aagtccacgc tgtactgatc gttgatgtcc caggagacgt tcaggatatg ggtctcggtg
     6481 tcgaagtacg tctggtaggg cggatcgtcg ttctgcagca ccgtgtagtc cttacccgac
     6541 tggggcgtct tcagcccctg ggcacactga ccgtaataca gacagaagat ctgatcaggc
     6601 tgcgcaatgt tgtgcagtgt gttggcgtcc tgctcctgct cggtcttgtc gtagcggtaa
     6661 tacagctcga tgctttcggt aggctgccag agcacgctgg ggctgatgac ccagtaatcc
     6721 atctccccgg cgtcgcgatc gagggtggcg ttcttgaacc agccgtcgct ctcgcgcttg
     6781 gcgccgccaa gacggaaagc gagattttcc gtagccgtga tgttgatgta agcatcgagc
     6841 tgcaggtcgc cgtggttgcc tgcaccggct ctgagcgacc cggacaggcc gtcgaagtcc
     6901 ggccgcgcgc gggtcacgtt gatgacgccg ccgatggaat tacggccgaa cagagtgccc
     6961 tggggtccgc gcaggatctc cacgctttcc aggtcgatgg ccttcagcat cgcacccgag
     7021 ttcaccccga tgaaaacgcc gtcgaccacc acacccaccg tggggtcgaa gttcttctcc
     7081 acgtccgtgg tgccgatgcc gcgaatcgag atggccgcgg ggctgcccgg accctgctga
     7141 atatcgctga cgatcaggtt gggcgaggcg ttctgcaggg tctgcaggtc cgcgtcgaag
     7201 cggatcttca gatcctcagc gccgagcgcg ctcagggcca tccccacttc ctgcatgctc
     7261 tcctcgcgct tccgcgcggt gacgacgatc tcctccaggg caagcgagtc ggactcggcc
     7321 agcgcgggcc caggcatacc gatgccgagc cagattaccg gtacgagcag cgacagcgct
     7381 cgtcccaaga ttgctctcct catgattctc tccagtaggc tagtttccga ttctgttatt
     7441 cgaacacacc gcgcagcggc tacccgcgaa cgctcgttcc ccttggtccc gcccaccggc
     7501 gggcagtggc atattataga cttgaggctg agcgcgccga aaccactcgc tcggcggcag
     7561 aaaacctgcg tcggctactc gaacagtcgc cgcaaccaga tgactgcacg ggccaccgtg
     7621 cccaggaagg gctgctccac cagctccacc accgtcccgt ccggatcgta aaacaggacg
     7681 atgcttcccc acgaatccgg ccccgagcag cacggggtga tcccggagat gaacttaaca
     7741 ccctgggcct tgagcgcagc cacgtcggcc tcgatgtcgc cggtgagcag cgccatgcga
     7801 tgaatgccga ggtggctgac cggcggcggg taggcgggct cggggtcgaa gggcgaaagc
     7861 cactggacca gctcgatctg cgagccgtcc acctcgtggc tgagcacggc cccgtcgatc
     7921 ctgtagggcg catcgaaccc catggccgct gccacctcgg cggagtccgt cggaggcagc
     7981 ttgcgggtgg ctacgtatcc gaacatccgg taccaggctt cggagcgttc gaagtcgctc
     8041 acgttgacgt ttacccgacc aacaccgatg acgggtttct cggatgcctc gccgcctggg
     8101 ccttccagct ctaccagctc gtagaaggtg ccgtccggat cggtgaagac ggcaaaccgg
     8161 gtaccgtccg agcgggtggc aggcggagag agaaagttta cgccggctcc cacgagctgg
     8221 tcgtaggcgg ccgcaatgtc gcccgtgtgc atgagagccc gcgccatgcc caggtggtta
     8281 aggcccgggt acggcggctc ttcgtttctc ggaatggtga actcgatgag atcgatgacg
     8341 ccgccccaga aaccatcggg gcccaccagc tcgccgtgga gcaggtagcc gccggcctct
     8401 tctccctggg agccgtcgta ggccgtcggc gtctggatgc ccagggcgtg ggccatcgcc
     8461 agggtgttgg tgtccggaaa gccgctcatg gtttcgaacc cgagcacgcc gtaaaacgcg
     8521 cgggcttcgt tgaaatcgct cacgttggtg ttgaagtgca cccaggagat gacgttcagc
     8581 gcgacgtccg cgcgctgctt gacgcgcacc gacgccgctg cgccgtcccg gccgtcgagg
     8641 ccaacggcaa tggtgttcgc ctcggcaagc tctaccggga tctccaggga ggtcgtttgc
     8701 cgtaacgcct gcccgtcgag cacgtcgacg ccgttcagcc ggacggtcac caccggctca
     8761 ccggatcgtg cggttacgat aacccgggca gggccggcaa ccccgaagaa cggccgcgcc
     8821 ggcccgacat cgggcccggg gtgctgcccg gccgcgcttt cgacccgtac cagatggcgg
     8881 gtgaagtcga tctcagcggc gaccgccaag acgggcacct gcaggcaaac ggcaaccagc
     8941 gcgaggaata tcccgcgcac gcgccgcggc tttgccccgg agcgatggtc cggcaaaagc
     9001 cgccgcgcgc gtgcggcgtc gtgccggatc acggagcgac caggagctta cccatgtggc
     9061 cgcccgtgaa cagcatggtc agcgctttgg ggaactgctc gatgccctcg acgacgtgct
     9121 cgggcatctt gagctttcct tcggccatcc agcgcgagat gtcggcctcc atctccggaa
     9181 agtccgccgc aaagcggtcc acggtgaagc ccagcatgct ggcattgcgc tcgggtatcc
     9241 tgagataaag gcgcggcccc ctgacctctt ccatgtggtt gtactgagag atggcgccgc
     9301 agatcaccac cctggctccc tgggcaatct gatcgagcac gatgtcgagc agctcgccgc
     9361 ccacgttgtc gaagaacacg ttcacgccgg caggcgcaag ggctcttagc ttctctgcca
     9421 cgtcgtcgtt cttgtagtcg atggcaccat cgcaaccgat ctcgtcgatc aggtaggcaa
     9481 ccttggccgg cccgccggcg atgccgatga ctttggcgcc ggctatcttg gccagctggc
     9541 acgcgatggt tcccaccgca cccgcggcgg cggaaaccac cacggtgtcg ccttcggcca
     9601 cctgtcccac cttgagcatg ccggcatagg ccgtaaggcc cgtggtcatc cccagcgcgc
     9661 cgagatagct gcgcggcggc acgtcgccct cctcgagctt ccggtacatg ccgcccggcg
     9721 cgacgaggtg agtctgtgcc cccatagggc cgaacacggc atcaccctgg ttcaggtcgg
     9781 gcgagcggct gtccacgacg cgcgccacgc ccagggccat cacgggcgta tcgatggcca
     9841 ccgtgccatg gagcccttcc tgggagtcca gcatcgttct gatgaaggcg tcgattgaga
     9901 gatgctccgt cgccagcagc acctgccctt cggtcagctc cgccagctcc tcctcgatga
     9961 ccttgaaatc cgactccttc ggctggcctt ccgggcgtga tgccagcact accttcttat
    10021 tgatcaacgt cgtctcctcg tcacgttgtt gttcgccatt aaggcggcta ctgggcgtcc
    10081 gcgacccagc tgccgtggaa tccggccggc acccgcacgg gcaggtgcac cctcgcgacg
    10141 gggtcggcgg tgacgttgct ggcatcgatg atcaccagct cgctgcggtt gtccgccggg
    10201 tcgtagacga agctcatgag atatccatcg tcttcggacg taccccctcg tgccggaacg
    10261 aagaccggct cgccgcccgc acggccgtcg gccagcacgt gcgcctcgga tgttcccgtg
    10321 gccaggtcgt acttacggaa ggccaccgcg gcgggaagcc caccaccgaa ctccgccgtg
    10381 tagccgtagc gatgcttctg accgaccagc gcatcgggca cgcgcgggaa atctaccgac
    10441 cgctcgtcca gctgcgcttc gctgacggtg cccgccgact gatgaatggt ccagcgatgc
    10501 agcatcggcg gcgcttccgg cgccccgatc ttcatgtggt gggccatgcg acagacgtca
    10561 atgacgatgt cgtcgccgtc ctcataggca ttcagcgggt ggtagacgta gcagggatcg
    10621 atgtcgtacc acaccacgtc ggcgttggag ccgttgcggg gcatcacgcc gaggcgcgcg
    10681 ccgtactcgt cgctccaggc cagcggaaga ccgacagagg cggcgttctc gaaattccac
    10741 accacgggca ggtccatgaa gatcacgtag ttccgcgtga tgttgaagtc gtggaccatg
    10801 gtcgcgccct tgacctcgat gacctcgctc tgaacgagct cgccatcggc ggtcgcgcgg
    10861 tgataggtca tgaagggggg cgtcgccgcg taagcaaaga agagcagctc gccggtctcc
    10921 gcgcagatct tcggatgggc cgtcatgccg gtctccagcc ggccgccgaa agtataagga
    10981 ccaacggtgt cgagctccgg ggtcagctcg aagggccagt gtccttcttc cagcgccaga
    11041 atccggcccg cgtggccaat gatgtgggtg ttggcaaggg acttggcgcg ctcgtccacc
    11101 ccatcgctgt ggtccccgtc gctgagcagg ggagtgcgga catagcggtt gcggtaccag
    11161 ttcgcccgac cgtcgttcag ctcgacgccg tgaatcatgc cgttgcccag aaaccagtgc
    11221 ggcgtgacgc cggattccgg gttggcgccg ttgcgcaggt aacgtccctt gagctccggg
    11281 gggatggtcc cggtcacctc gagcctggtc tcggtcacct cgtcaaaggt cggggcgaaa
    11341 ttgcccgaga gccaaaaggg tgcttcgttg ctgactgctt cgctcatgtt gcgtctcctc
    11401 gactgagttt cgttgcgtcc gccagcggcg gtcgttcctc ggctccgctc ggggccagca
    11461 tggctttcat gcagccgacc gcttcgcgga tgtaatcttc tctgcggtcc ggatcaccgc
    11521 cgtcccgcca tgccttgtag gccgcgtcca ccagggcgaa ctgcagctcg gccatgattc
    11581 cggcatcccc ttcccggacg atgccctgag ccattccagc ccgcaggctc ttctcgatgg
    11641 ccgcgacgat gacagcgctg cggctggcgc cactcgaccc gccgagttct cggctcatct
    11701 gctcgagcag ccggtacagt gccggatggg catcggcaaa atcgaacgcc ccccgcacca
    11761 ccatctcctc gctgagctgg cccggcgtat cgggcatggc cgcctgaacg ccggcggcca
    11821 cgaaatcctc tgcgatgcgc tcgagcagcc ctcgtttcga accgaagtgg tgaaagagga
    11881 tgccttccga gacccccgcc gtcttggcga tctgctgagt cgaagtgtct tcgaacccgc
    11941 gctcggcaaa cagctcccga gcggctgcca gcaatcgctc ccgcttttcc gcaggggccc
    12001 tggtgtaggt aaccgattta gagtgcgcga ccatttattg agtagcactt tacaaagtgt
    12061 tactcaacaa gtaaaccccc aatttgcggc atgcaggttt tcagtcgttc tgctggccgc
    12121 ggcccgccga gcgagccgcc ccaaggcgcc gccggacgag gtcgatgtcg cggcgaagct
    12181 ggtagaggtc gtcggcatag cccttcggaa ccgcctcgat agtcgagtcc acgtccagcc
    12241 gatcgagctc ctccagccga cgggccacgt cctcatcgtc ggcgatgggc gttcgaagcg
    12301 gatccagggc gtgcagcttc gtgtagatgc gcagcagccg ccgacggatc tgccatcgat
    12361 acgcaggggg caccagccgc gccagcggca ggctcaaccc gatcagcggg acgagcatga
    12421 tccagagccg gtcaaccagc gtggccgccc agaacggcag gtagcgcatg agaaacggcg
    12481 ccccgtgccg gtagtgcctc tccgcctcct cgctgagcgg caggtcgacg aaacgcggcg
    12541 tgggaaacgt gcccgcatcc gcgagaatgc tgtgaccacc gtgtatctcg gcggccgcga
    12601 tgagcagaag gtccgtgagc gccggatgga aatcatcacg ggccacgagc atggccccca
    12661 cggcaaccgt ctcggtgtcc ttcgccggca ggtccgcttc cagatccaga acgcccctgg
    12721 gcaggacgac tgaagagagg aaggcgttgc gccgcgtgta agcctcgacc cgttccatgt
    12781 cgtgcagacg aactcccggc aggcggagaa gcttcccgac cgtggaggat tgcggcgcgc
    12841 cgatgagaaa cgccgcgtcg atctcgcccc cagccagcgc gtcgggaagc gcggctgacg
    12901 ccacccccag aagcttcgag ttcgccgcgt ccaccccgtt ggcctcgagc agctgcatca
    12961 cgacggcgcg tgttccgctg ccttccacac ccaccgccaa ccgccggccc ttcagatcgc
    13021 ccaggtcgtc gagcaccacg cccggtccaa ggaacagcca gaggggttcc agatacaggc
    13081 tgcccatagt gacaaggttg tccacggggg cagacgacgc gatgcccccc tgcacgaaag
    13141 cgaggtcggc ccccccttcc ctggccacca gatcgagatt gtccacgctg ccaccggtgg
    13201 cgcgcagttc gaccaccacc ccgtcccggg ccagaacctg ggcaaaccgc tcaccgaaat
    13261 agtagtaagc gccggacggc tccccggttg cgagaacgaa ccgtttcggc ggcggcggcc
    13321 cgacgaactg ataggcgacg atgagccccg ccagcaggag cgcgagcgcg agcccccagg
    13381 ttcccgctac gtccctggtc cgggcccaga attcagctgc acgttgattg gacatgctcg
    13441 aacggtcaac gcttcttctt caggcgctca tggccgcgcg aatcctcgtc ggccttctga
    13501 aattccaccc cgagcacctt caacgtcccc tccgactcct cggcttcctg caactccccg
    13561 ggcgctttgg aggcagcctc ttcggtcggg tcggccgtgc tgaccacccg tctggggctt
    13621 tccgaaatca tgccgccgca gaattcccca agcacctcgc gggctcgcgt ctgctgttct
    13681 tccgacagcg cgcccagcac ttcggattgc aggcgctgta gtacctgatc gacatcgcaa
    13741 acctctgcgc ttgccgaagc cgcgccgatc gcgagcccga agacgacgag aacggccccg
    13801 gtttgaatcg agctggtcat ggcggcatta tgcaccaccg cggctcagac gtgctgccgt
    13861 tcgtgcacga gaggccaccc ttttcttcgt tacgcccaca gtcaccacga cgggcacggt
    13921 attgctcgag gagccaccat gtacgaccac ctgatcagga acgccaccat cgtcgacggt
    13981 ctcggcggaa agccatgcac gggcgacatt gccatcaagg atggcgtcat cgccgcggtg
    14041 ggcgaagtct ccggaaacgc cagggagacc ctgaacgcgg agggcgccta cgccactccc
    14101 ggctgggtgg acgtgcatac ccactacgac ggtcaggtca gctgggacga taccatcgag
    14161 ccgtcgttca gccagggtgt gaccagcatc gtcatgggca actgcggcgt cggcttcgcg
    14221 ccgtgccgcc cgggggacga aaagcgcgtg atcgagctga tggagggggt cgaggacatc
    14281 cccggcaccg cactgtacga gggcatcgag tggggcaact gggaatcgtt ccccgagtac
    14341 atcgagtacc tttcgaccaa gcggtacacc atggatatcg gcacccagat tccgcacagc
    14401 gctctgcgta actacgtgat gggcgagcgg gcactcaccc acgaggatgc caccgccgag
    14461 gacctcgaag ccatgtgccg gctggtccag gacggtctcg ccgccggagc cctgggattc
    14521 tctacctcga gaaccatcgg ccaccggtcc gttgacggcg agcccatccc cgggacgttc
    14581 gccgagaaag acgagctgct ggccatcgcc cgggccatga gggaggccgg acgcggcgtc
    14641 ttccaggccg tgccggccgg ggtcgtcggc gacatcgcag ggccggagcg atggacgacg
    14701 gagcaggaag tcgagctttt cgccgaggtg gcccgagcca gcggccgcaa gtgcacgttc
    14761 accctgattc agaacggcaa caggcccgat cagtggcgca actgcctgaa catcgtggag
    14821 caggccaacg ccgagggcat cgagctcacg cctcaaatcg cgacacgccc catcggcatc
    14881 gtcacgagcc tggactccta tcacatgttc atgcgccggg agacctatct gaagtatgcg
    14941 gacctgcccc tgtcttcgcg gctcgcggag ctgagaaagc ctgaggtgag ggccgcgatc
    15001 ctggcagaca aggacgtccc gccggaccag ccgggcgtga tggccaacgt cttcgccctg
    15061 atgcagatgg ccgcaccagc catgtttccc atcgacatcc ccatcgacta cgagccggag
    15121 ccgtcggcaa acatgggcgc gcgtgcagcc gctgcgggca tggacatcca agccttcatg
    15181 tacgattttc tcaccgccgc agacggcgac cagttcgcca ttctgctcgg cgcgaacttc
    15241 gccgacggta ccttcagcgt gatcgaggag atgattcccc accccaacac gaccatcggc
    15301 ctttccgatg cgggtgcgca cgtcaatctc atcttcgacg ccgtggggcc cacctaccag
    15361 ctcacacact gggtgcgcga ccgcagccgg ggcagacggt ttcccatcga gctgatggtg
    15421 cacaagcaga cctacagcaa tgccaggctg ttcggcctga acgaccgggg cagcatcgag
    15481 gtcggcaagc gggccgacat caacgtcatc gacctggagc ggttgggcct cggcaagctg
    15541 cacgtgcatc gagatctgcc ggccggcggc agccgcatcc tgcagcccgc ccacggctac
    15601 gtcggcacgt tcgtggctgg agtgaaaacc cgggaaaacg atcgcgacac cggcgcgcgg
    15661 ccggggcggg tgatccgata gctacgctag cggtggtctt ccggagctgc tggtcctgcc
    15721 aatagagcag gcgtgcgacc gaaaaaaccc gggttgagac ccgcaaagcg gctcgatacc
    15781 ggattcattg agcaacggca gcacgcaaca aggccgctat ccgctttgcg ttgcgtcggc
    15841 agtcgtatgt cgcctccaca atttccctgc ctgccatccc gagggcgcga ccccgatcag
    15901 ccgcatcgcg cagaccctgc aaagccgcga tccactcgtc ggtttgcgag gcaagaaagc
    15961 cgttcacacc ttcgcggacg atatgctggt tttcccctac cggacttgca accaccggga
    16021 gcccacaggc catgtactgg atcagtttgt aacccgactt gccgcgctcg aagggcaggt
    16081 ccggaagcgg catgattccg caatcaagct gttgcagcag gctgacttcg tcactctccg
    16141 tccaggggaa cgcttcgtag ggcaccccga cgtcaagacc ggctggcacg ccgataaacg
    16201 taaaccggaa cccgcccgat ccgtgcagcc gttcgaacac gggggtcaat gtcttcagaa
    16261 acggcgccgt ctgcggcgtc ccgatccaac ccacgttgaa gtcacccgat gcgtttcttc
    16321 cagaatcctt cgccagcggc cggtatcgct gcgtatccac cgaggtcggg acgatctcga
    16381 gttcacgggc accggcacgg cgcgcggcgt cggcaaggta acgattgcca accaccacga
    16441 cttgcgcgcg acgcatcacg agatcgatct tgtttccgag cagccaccgt accagcgccc
    16501 gcgggtgctg atcgtagcgg tgaaatatcg cgtcgtcgta gtcgaccagg tacggccgcc
    16561 gtaacatccg ttcgaacgct gcggggagcc agggcagcag ttccttctcc agccatacca
    16621 ggtcgtaggc acgctggttg gtcaacgcag cgagccggcg cgtgtagccg gcgagggaag
    16681 ccagaagcgg cccccgcccg cggcgataca gcccctcgag ataggcgtcg accaagagag
    16741 gcgaaacatc tatgtcaatg ccgtcctcac gcagaagcgg aacgtattgc agcgttcgca
    16801 gccgggtgct ggctccaagc gcgccatatc tcgtcaaggc gagaacgcgg atcacgagcc
    16861 cagccaacga tatagtcgcc cgtagccacg caggacctcg ccacaacccg ttaccgaacc
    16921 agccagcacg ctggccagaa tccgcaacca gggctcgatc gcgagcgtgc tgaacccaac
    16981 gatgatcagg ccccacacgc ccatatgctt gtgcacatac tgcaaccgcg acctcaacat
    17041 gtagaaaagc ctctcgtcct tgatctgtcg ggatgaacca cctccgacgt ggctggcgca
    17101 gggattggct agatagagaa tgtccatccc ggcctgctga attcgcgccg aaaagtccag
    17161 atcctcgagg tagaggaaaa atcgctcgtc gaatccgccg aggcgatcaa atacagcgcg
    17221 tggcgtcagg tagcaggccc cgatcacgtg gtcgactttg cgcgagctgg aatgatccca
    17281 tccactcaga cgatagccca gtcgcggcca gagacgatct atgccaagag aacgcgcgag
    17341 catgctgatt ggcgtcggta accggcagca cgttcgctgg cgctggcctg aaccagcgtc
    17401 atacaaacca atgcccaccg cgccaatggt ggcgtcccca cggagccggc gcagcggttg
    17461 ggtcaatatg tcctggccga tgaccagatc cggattcgcg aacagcagga aatccgcccc
    17521 agcccgcgac gctccgagat tgcaggcggc tgcgaatcca aggttgcacc cggagcggat
    17581 cagatcgacc tccaatgagg tcggcatcgg aaagtccaga gaagaatccg tggatgcatt
    17641 gtccacgatg atgacctgac caattacgtt cgcgcagtgg gc
//
LOCUS       DBNHLM010000264         6963 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_157945, whole genome shotgun sequence.
ACCESSION   DBNHLM010000264 DBNHLM010000000
VERSION     DBNHLM010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6963)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_157945"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="AC16QF_12325"
     CDS             <1..338
                     /locus_tag="AC16QF_12325"
                     /inference="COORDINATES: protein motif:HMM:NF048554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6449391.1"
                     /translation="ITLVSGSPARKCPPGFAAISSVGNAVEGFVRAVAAEIVPRRINA
                     VSPGFIDTPMFPVEGEAREKFLAQATAGNLIKRPGTAEEVAQAILFTIRNDFVTGTTV
                     DVDGGALLP"
     gene            335..1357
                     /locus_tag="AC16QF_12330"
     CDS             335..1357
                     /locus_tag="AC16QF_12330"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008126 - acetylesterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HIR6449392.1"
                     /translation="MRFAGEQPFRPPRLLSNGHVQTVLGNRVRQSWVERKAQPMKATS
                     DALVLCTSEGVRLQALASLRDGAPTVVIIHGWLGHADSSYVLSAAGELWAQGFSVIRL
                     NLRDHGGTAHLNEEMFHSARTREVVEAVTQLSTDFATGPVGVLGYSLGGNFALRVARA
                     TGLPTLAVCPAVDPDRTMQAIDQGWMGYRLYFVRKWQQALASKQQAFPARYDFTRALE
                     LRSVSALTDHFVRDHTDYPSTRAYFDRYTLTGPALSDTEATVVYAADDPVIPVAGFAD
                     LAPGIELVPVARGGHCAFLDHPRRASWIDRYAADHFARRLGPAQITPDAPSSARRSAA
                     IPSSSP"
     gene            complement(1291..2478)
                     /locus_tag="AC16QF_12335"
     CDS             complement(1291..2478)
                     /locus_tag="AC16QF_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6449393.1"
                     /translation="MSSEGPLHGIRVVELGQLIAGPFCGQLLGDFGAEVIKVEPPGAG
                     DPMRGWGQGAPVWWPVIARNKKSVTLNLREAEGQELLKQLAVKADVLIENFRPGTMEK
                     WGLDYGTLAALNPGLIMVRVSGFGQTGPYAHRAGYGSIGEAMGGIRYLAGDPDRPPSR
                     VGLSIGDSLAATFACLGTLAALHSRHATGRGQVVDSAIYEAVLAMMESTIPEFTEAGV
                     IRERSGAILPKIAPSNVYPTADGEMILIGANQDSVFARLCRAMERLELTEDARYRDHA
                     ARGEHQQELDELIARWTRTLAADALLDVLESNGVPAGRIYRAPEMLADPQYAARESIV
                     AVAHRAFPNLKMQNVAPKLSETPGRIRWHGPELGEHNEEIYGELLGIAADRRAELGAS
                     GVI"
     gene            complement(2468..3403)
                     /locus_tag="AC16QF_12340"
     CDS             complement(2468..3403)
                     /locus_tag="AC16QF_12340"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HIR6449394.1"
                     /translation="MSEADQRAVTVMEVSPRDGLQNEAEVLSTQNKLALIDLALEAGC
                     RRLEAVSFAHPARVPQMADAEAVCLGLPQRGDVIYTGLVMNQRGYERLLATERLDEAG
                     LVVPATDTFGQRNQGMDVAANVAMARSVLRDARLRGLRAQATIAVAFGCPFEGEVLVQ
                     RVVAIASAIAEEGPVEIGLADTVGVGVPGQVLEAFSAVAEAVGPDIPLRAHFHDTRNT
                     GIANAFAALQAGVATLDASIGGIGGCPFAPNATGNIATEDLVYMLDRMGVDHGLSLER
                     LLAGAEWLQGVLGRPVPSSLLRAGGFPQPEPVDVV"
     gene            complement(3449..5086)
                     /locus_tag="AC16QF_12345"
     CDS             complement(3449..5086)
                     /locus_tag="AC16QF_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HIR6449395.1"
                     /translation="MAEEPNDFIRQRIRADLASGVVKGGVVTRFPPEPNGHLHIGHAK
                     SICLNFGVAEEFGGYTYLRFDDTNPLKESEEFALAIQEDVRWLGYDWGDHLTHASDYF
                     EELYGFAAQLIEQGKAYVCSLTGDEIRATRGSLTEPGTESPYRDRSVEENLDLFRRMR
                     EGEFADGEHVLRAKIDMASPNINMRDPVIYRIRHASHQRTGDAWVIYPMYDFTHCICD
                     ALEGITHSLCTLEFEDHRPLYDWVLDNININFHPPQIEFSRLGLEYTVMSKRLLHRLV
                     AEGLVEGWDDPRMPTIAGLRRRGVTPGAIREFCRRIGVTKQDGMVEMNLLEFCIRQDL
                     ESSAPRGMAVLDPLKVVITNHPGAEEYLVAPWHPNEAELGERELPFGPEIYIERADFM
                     EEPPRKYKRLSPNEMVRLRYAYVIRCDEVVRDEVGNIAELRCTYFSDSKSGADTSGLK
                     PKGVIHWVSAATGMPTRIRLYDRLFTVPAPRGEELVGELNTQSLAEMSGFVEPAVAAS
                     AARRFQFERQGYFCKDSQLDGVFNRTVTLRDSWKPAP"
     gene            5184..6587
                     /locus_tag="AC16QF_12350"
     CDS             5184..6587
                     /locus_tag="AC16QF_12350"
                     /inference="COORDINATES: protein motif:HMM:NF013961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR6449396.1"
                     /translation="MTLRLIVAGAICIALGAGSVLLEQHASQAVQSARHFLGERWYVL
                     ELGGRQVGYLHTGASHDREGQWQFRSELRFALQPDEAVTVEDELTFAAVAPHPLSEAT
                     HRISQPGSTQLVVIRRDATHYSAIVSSTEGERGEPTARLDWSYALGDYLAFETWLRTA
                     SPPPGAARTVPTLNFSRLAPFAKAYRIAERNPTGYRVSSPDPLDETIIQLDDDHLPVT
                     LEMAGLFKLRRTSRDLAVGGRSHLKAATYYVPADQRLPNHTQIETIVLGVHGAPDARR
                     LWPQAEGRSGSWTLTLNANPVSSDADPRDHTATTLEFPATDERIVELARTAVAGPSGQ
                     AERVAALTRFVHHYLTYAPGGASRPVLALLDEPVGDCTEHADLFTTLARSLNIPARTV
                     CGLAYADREEPAFAFHAWNEVSLDETWRPVDPTWNLLRVDATHIPLPSNRGAALALIT
                     GATKLSFSVLEVGYFDD"
     gene            complement(6584..>6963)
                     /locus_tag="AC16QF_12355"
     CDS             complement(6584..>6963)
                     /locus_tag="AC16QF_12355"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF044908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6449397.1"
                     /translation="GSDEWAANRHWNPVWIRFAGEVDPDDDPLARSLLAYASDVGLVS
                     TGVLPHVESISRAEVRMASLDHALWFHRAVPIADWLLVHKRTTMARGGRALIQAEIFS
                     RDGKLVASVSQEGLIRRRSAAPG"
BASE COUNT         1177 a         2434 c         2206 g         1146 t
ORIGIN      
        1 gcatcaccct ggtgagcggc tcgccggcac gaaagtgccc ccccggcttt gcggcgatat
       61 ccagcgtcgg caacgcggta gaggggttcg tacgagccgt cgccgccgag atcgttccgc
      121 gacggatcaa cgcggtgtca cccggcttca tcgacacgcc catgtttccg gtagaaggcg
      181 aggcgcgcga gaaattcctc gctcaggcga ccgccggcaa cctgatcaaa cggccgggca
      241 ccgcagaaga ggtcgcccag gccatcctct tcaccatccg caacgacttc gtgacgggca
      301 cgacggtgga cgtcgacgga ggcgccctgc tgccgtgaga ttcgccgggg agcaaccatt
      361 tcgaccgccc cgcctgctga gcaacgggca cgtccagacc gttctcggca accgggtgcg
      421 acagtcctgg gtcgagcgta aggcgcagcc gatgaaggcg accagcgatg cactcgtgct
      481 ctgcacgtcc gagggcgttc ggctccaggc ccttgccagc ttgcgagatg gcgcgcccac
      541 ggtggtcatc attcacggct ggctgggtca cgccgattcg agctacgtcc tttccgccgc
      601 cggcgagctc tgggcacagg gtttttcggt gatccggctg aacctgcgcg atcacggcgg
      661 gacggctcat ctgaacgagg agatgtttca ttccgcacga acccgcgagg tcgtggaagc
      721 ggtaacacag ctgagcaccg acttcgcaac cggccccgtc ggcgtgctgg gttattccct
      781 gggcggcaac ttcgcgctgc gggtggcgcg cgccaccggt ctgccgaccc ttgcagtctg
      841 tccggccgta gacccggatc ggaccatgca agccatcgat cagggctgga tgggctaccg
      901 cctgtacttc gtccgcaagt ggcaacaggc gctggcttcc aagcagcagg cattccccgc
      961 acgctacgac ttcacccgcg ctctggagct gaggtccgtc agcgcgctga ccgatcactt
     1021 cgtccgcgac cataccgact acccgagcac gcgcgcctac ttcgaccgct acacgctgac
     1081 cgggccggcg ctgtccgaca ccgaggcaac cgtcgtctac gcggcggacg atcccgtcat
     1141 cccggtcgcg ggcttcgccg atctggcgcc gggaatcgaa ctcgtgccgg tggcgagggg
     1201 cggtcactgc gccttcctcg accaccccag gcgcgccagc tggatcgacc gctacgcggc
     1261 cgatcacttc gcccgacgac tcggcccggc tcagatcacc cctgacgcgc cgagctcggc
     1321 gcgacggtcc gcagcgatcc ccagcagctc gccgtaaatc tcttcgttgt gctcgccgag
     1381 ctccgggccg tgccagcgga tacggccagg cgtttccgag agcttcggcg cgacgttctg
     1441 catcttgaga ttgggaaatg cccggtgcgc tacggccacg atggattccc gcgccgcgta
     1501 ctgaggatcc gcgagcatct ccggcgcccg gtagattctc ccggccggca cgccgttcga
     1561 ctccagaacg tcgagcaacg catccgccgc cagcgtccgg gtccagcggg caatgagctc
     1621 gtcgagctcc tgctggtgct cgccccgggc cgcgtggtct cgatagcgcg cgtcttccgt
     1681 aagctcaagc cgctccatgg cgcggcaaag gcgggcgaac acggaatcct ggttggcccc
     1741 gatgaggatc atctccccgt cggcagtggg gtagacgttg gaaggcgcga tcttcggaag
     1801 gatggcgccg ctgcgctcac ggatgacgcc ggcttcggtg aactcgggaa tcgtcgactc
     1861 catcatggcc agcacggctt cgtagatggc cgaatccacc acctgccctc gtccggtcgc
     1921 atgccggctg tggagggccg ccagggtgcc caggcaggcg aaggtggctg ccagggagtc
     1981 gccgatggaa agccccacgc ggctcggcgg ccggtccggg tcacccgcca gatagcgtat
     2041 accgcccatg gcctcgccga tcgagccgta gcccgcccgg tgagcgtatg ggcccgtctg
     2101 tccgaatccc gacaccctga ccatgatgag gcccggattc agcgcggcaa gagtgccgta
     2161 gtccaggccc cacttctcca tggtgccggg ccggaagttc tcgatcagca cgtccgcctt
     2221 gaccgcgagc tgcttgagca gctcctgccc ttctgcctcg cgcaggttca gcgtcaccga
     2281 tttcttgttg cgcgcgataa ccggccacca gacgggcgcg ccctggcccc acccgcgcat
     2341 gggatccccc gcaccgggag gttccacctt gatcacttcc gcaccgaagt cacccagcag
     2401 ctgaccgcag aaaggcccgg caatcagctg gcccagctcg acgacgcgta tgccgtgcag
     2461 gggcccttca gacgacatcg acgggctcgg gctgcggaaa accgccggca cgcagaaggc
     2521 tcgacggcac cggacgacca agcaccccct gcaaccattc cgccccggcc agaaggcgtt
     2581 cgagggacaa cccgtggtct acgcccatgc gatcgagcat gtaaacgaga tcctcggtgg
     2641 cgatgttgcc ggtggcgttg ggggcgaagg ggcaaccgcc gataccgccg atgctggcgt
     2701 ccagggtggc aacccccgcc tgcagggcgg cgaaggcgtt ggcaatgccg gtgttacggg
     2761 tgtcgtgaaa gtgggccctc aacgggatgt ccgggccgac ggcttctgcc accgcgctga
     2821 acgcctcgag cacctgaccg ggaacgccga cccccaccgt gtcggccagg ccgatctcca
     2881 ccggtccctc ttcggcaatg gccgacgcga tcgccacgac gcgctggacg agcacctcgc
     2941 cctcgaaggg gcaaccgaac gccacggcaa tggtcgcctg ggcgcgcagc cctctcaaac
     3001 gcgcgtcccg cagcaccgaa cgggccatgg cgacgttggc ggcaacgtcc atgccctgat
     3061 tgcgctggcc gaacgtgtcg gtggccggaa cgaccagccc ggcctcgtcg agacgctcag
     3121 tggccaaaag acgctcgtag ccgcgctggt tcatcaccag gccggtgtag atcacgtctc
     3181 cacgctgcgg caggcccagg cacacggctt ccgcatcggc catctgtgga acccgagcag
     3241 ggtgggcaaa gctgaccgcc tccagccggc gacagcccgc ctcgagggcc aggtcgatga
     3301 gggccagctt gttctgcgtg ctgagcacct cggcttcgtt ctgcagcccg tcgcgggggc
     3361 tcacctccat gacggtcacg gcccgctggt cggcttctga catttacgtg acctcctggt
     3421 tatgccctga acggaccacc gcgacggcct aaggagcggg cttccagctg tcccggagcg
     3481 tcaccgtgcg gttgaaaacc ccatcgagct gagagtcctt gcagaaatag ccctgtcgct
     3541 cgaactgaaa ccttctcgcg gcgctcgccg ccaccgccgg ttcaacgaaa ccggacatct
     3601 cggccagcga ctgcgtattg agctcgccga cgagctcctc gccacgcggc gcggggacgg
     3661 tgaagagccg atcgtacagc ctgattcgtg ttggcatgcc cgtggccgcc gatacccagt
     3721 gaatgacgcc tttgggcttg aggccgctgg tgtccgcgcc gctcttcgag tcggagaaat
     3781 aggtgcagcg cagctcggcg atgtttccca cctcgtccct gaccacctcg tcgcaccgga
     3841 tcacgtaggc gtagcgcagg cgcaccatct cgttgggaga cagacgcttg tacttcctcg
     3901 gcggttcctc catgaagtcg gcgcgctcga tgtatatctc cgggccaaac ggcagctcgc
     3961 gctcgcccaa ctctgcctcg ttgggatgcc acggcgccac cagatactcc tcggcgcccg
     4021 ggtggttggt gatcaccacc ttgagcgggt ccaaaacggc catgcctcgg ggcgcggagc
     4081 tttccagatc ctgtcggatg cagaactcca gcaggttcat ctccaccata ccgtcctgct
     4141 tggtcacccc gatacggcgg cagaactcgc gaattgctcc aggggtcacc ccacgacgcc
     4201 tgagacccgc gatggtcggc atgcgcgggt cgtcccagcc ttccaccagc ccttccgcca
     4261 ccaggcggtg caggagacgc ttgctcatga ccgtgtactc gagccccagg cgggaaaact
     4321 cgatctgcgg cgggtgaaag ttgatgttga tgttgtccag cacccagtcg tagaggggcc
     4381 ggtggtcctc gaattccagc gtgcaaagcg agtgcgtgat cccttccagg gcatcgcaga
     4441 tgcagtgcgt gaagtcgtac atcgggtaga tcacccaggc atcacccgtt cgctggtgag
     4501 aagcatggcg gatccggtag atcacgggat cgcgcatgtt gatgttcggg gacgccatgt
     4561 cgatcttcgc ccgcagaacg tgctcaccgt ccgcaaactc gccttcgcgc atgcgccgga
     4621 agagatcgag gttctcctcc acggatcggt cgcgataggg actctcggtg ccgggctcgg
     4681 tgagggaccc ccgggtcgcc cggatctcgt caccggtcag gctgcagacg taggccttgc
     4741 cctgctcgat cagctgcgcg gcaaagccgt agagctcctc gaaatagtcc gaggcgtggg
     4801 tgaggtggtc gccccagtcg taacccagcc agcgcacgtc ctcctgaatg gccagggcga
     4861 actcctcgct ctccttgagc gggttggtgt cgtcgaagcg aagataggtg tagcccccga
     4921 actcctcggc aaccccgaag ttcaggcaga tggacttggc gtggccgatg tgcagatgcc
     4981 cgttcggctc cggcggaaat cgagtcacca cgccgccctt cacgacgccg ctggcaaggt
     5041 ccgcgcgaat gcgctggcgt atgaagtcgt tgggttcttc ggccatcggc ccatctacgg
     5101 aatctgcggg gcgcgtattc taaccaaagg ccgaaaccgg cgtctgctgc taatattctt
     5161 cgggaaagca cagggggagc ttcgtgactt tacggttgat cgtcgccggc gcgatctgca
     5221 tcgcgctcgg cgcgggatcg gtcctgctcg agcaacatgc ctcccaagcc gtccagagcg
     5281 cccgtcactt cctcggtgaa cgctggtacg tcctggagct cggcggtcgc caggtcggct
     5341 acctgcacac cggcgccagc cacgatcgcg agggccaatg gcagttccgc agcgagctgc
     5401 gattcgcgct gcagcccgac gaggcggtca ccgtcgaaga cgagctgacc ttcgctgccg
     5461 tggcacctca ccctctctcc gaggccactc accgcatcag ccagccaggc agcacacagc
     5521 tcgtcgtcat caggagagac gcgacgcact actcggcgat cgtctcgtcg actgaagggg
     5581 aacgcggcga acccacggcc aggctggact ggtcttacgc gctcggcgac tacctggctt
     5641 tcgagacctg gctgaggacc gcctcgccgc ccccaggcgc cgccaggacc gtgcccacct
     5701 tgaatttctc ccggctcgcg ccgtttgcca aggcgtaccg gatcgcggag cgaaacccga
     5761 cagggtatcg cgtgtccagc cccgaccctc tggatgagac catcatccag ctcgatgacg
     5821 accacctgcc cgtcaccctg gaaatggccg gactgttcaa gctgcgccgc accagccggg
     5881 acctggccgt gggcgggcgc agccatctga aggccgccac ctactacgtt cccgcagacc
     5941 agcgactgcc caaccacacc cagatcgaga cgatcgttct cggcgtgcac ggcgccccag
     6001 acgcccgccg tctctggccg caggccgagg gccgatcggg gagctggaca ctgacgctga
     6061 atgccaaccc ggtgtcatcc gacgcggatc cacgcgatca caccgcaacg acgctagagt
     6121 ttccggccac tgacgagcgc atcgtcgagc tcgcgcgcac cgcggtcgcg ggtccgagcg
     6181 ggcaagctga gcgggtggcc gcgctcaccc ggttcgtcca tcactacctg acctacgcgc
     6241 cgggcggggc ctcgcgtccc gtgctggcgc tgctcgacga accggtcggc gactgcacgg
     6301 agcacgccga cctcttcacc accctggccc gcagcctgaa cattcccgcc cggaccgtct
     6361 gcggtctggc ctacgccgat cgggaggagc ccgcgttcgc cttccacgcg tggaacgagg
     6421 tgtccttgga cgagacgtgg cggccggtgg acccgacatg gaacctgctt cgcgtcgacg
     6481 ccacccacat accgctgccc agcaatcgcg gggcggccct ggccctgatc acgggcgcaa
     6541 cgaaactcag cttttccgtg ctcgaggtcg gctacttcga cgactagccg ggggcggcgc
     6601 tgcggcgtct gatcagaccc tcctgggata cggaagcgac cagcttgccg tctcgtgaaa
     6661 atatctccgc ctgaatgagc gcccgcccgc cccgggccat ggtcgtcctt ttgtgaacga
     6721 gcagccagtc ggcgatgggc acggcccgat gaaaccacag cgcgtggtcc aggctcgcca
     6781 tgcgcacctc ggcgcggctg atcgactcca catggggaag caccccggtc gagaccaggc
     6841 ccacgtcgga ggcgtaggcg agcagcgacc gggccagcgg gtcatcgtca ggatccactt
     6901 cgcccgcgaa gcggatccac acggggttcc aatgccggtt tgccgcccac tcgtcgctgc
     6961 ccg
//
LOCUS       DBNHLM010000265         4056 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1236469, whole genome shotgun sequence.
ACCESSION   DBNHLM010000265 DBNHLM010000000
VERSION     DBNHLM010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1236469"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="AC16QF_12360"
     CDS             complement(<1..876)
                     /locus_tag="AC16QF_12360"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR6449398.1"
                     /translation="MSKGSRKRLTLGLVLGVGALGVALGWPIPDSERWEFGPDPKLAA
                     GKTSYLSALAAETFDKRPNVLLIVTDDLGRYDLSAYGHTAAHTPNIDALARDGVQFLS
                     AFATAAICAPSRAALLTGRYQNRFGFESQPMQRYVRNLGEYLGFRYLIDTDEMQPFLL
                     DTYPNAEQRLRQGLPASEISLGDALDAAGYATGIFGKWHLGYGPENHPQAFGFEQQYG
                     FTEAFSLYANEGAPGIVEHRHELFWEEHIWNMGREGPSAITRNGEPIAETRYLTDAIV
                     EETKRFISDARVQDRP"
     gene            complement(889..1659)
                     /locus_tag="AC16QF_12365"
     CDS             complement(889..1659)
                     /locus_tag="AC16QF_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIR6449399.1"
                     /translation="MTVDVRQVGRVTVVTIDRPEVRNAVDGPTADALAEAFSAFDAND
                     ASDVAVLTGGDGVFCAGADLKAVAAGAGPRLRETGNGPLGCTRMLLSKPVIAAVEGFA
                     VAGGLELALWCDMRVAASDAVFGVYCRRWGVPLVDGGTIRLSRLIGHSHALDLILTGR
                     GVSGDEAKAMGLANRIAPPGEALEAAMKLARSLTEFPQLCLRNDRLSSYRQWSMPLED
                     ALREETRLGLEVIRSGETHKGAARFADGKGRHGDFGDI"
     gene            complement(1683..2948)
                     /locus_tag="AC16QF_12370"
     CDS             complement(1683..2948)
                     /locus_tag="AC16QF_12370"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6449400.1"
                     /translation="MSGNRPTIDPAVFASAEYAADPYPTLKLLRDQYPVYNVPGTDHW
                     IITRYEDVVNGFRDTENFSASPNGESIGAVFGPTLMEYDGDDHTKLRNIVAPQFVGKK
                     LKALLPTVGRNAMALINAFTELHARRIAGQAAAVGEIDIVDDFATRLPLNVILDVLDL
                     PQEAHEMFHQWYPAMMNGINGGPELRALGVAANAEYHAYLDPLIDARSRNPADDLLSR
                     LAVAVVDGQRMTKEEIKSFASLILVAGAETTDKAIANLWYQLLANPEQWKAVQADPDL
                     LDQAFSEMMRIHPPASAQARRAKRDVELHGKVIPAGAYVQLSIYGANRDERVFRDPDR
                     FDLFREDLYFGKELRSGYHQQGMAGHLGFGLGKHFCVGYQLARAETVIGTRMLMEVIR
                     NPRFKTGSKPTPIAIRIEPWSLPLEFDVA"
     gene            3032..3415
                     /locus_tag="AC16QF_12375"
     CDS             3032..3415
                     /locus_tag="AC16QF_12375"
                     /inference="COORDINATES: protein motif:HMM:NF016211.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HIR6449401.1"
                     /translation="MRRKTTKLLFITGGWLSLALGIAGIPLPLLPTTPFLLLAAFCFA
                     EGSDRLHDWLVNHVHFGPPIRRWREHRAVSRRTKWMGSLSLLAVLAVSLGFGAPLWLL
                     AVQGCVVVGGGAFLWTLPEPPAEEQ"
     gene            complement(3469..>4056)
                     /locus_tag="AC16QF_12380"
     CDS             complement(3469..>4056)
                     /locus_tag="AC16QF_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6449402.1"
                     /translation="FAEYKDLQRGVIREANTSTGQETFVDNAAGAESFGAELSFSFVP
                     VAGLNINGNIGYLDIEYKGFVADLTGDGVETDNSDLDLVRAPKWDMSIGASYEFDLAD
                     MGNLTIGARFSYTDDMVLTTPNDVGFYRDELTTIDAQAVWESANRRYRFSVWGKNLSD
                     NIERLGGTPVATLFAFAAPTQPRQYGATLAVMLGD"
BASE COUNT          645 a         1292 c         1383 g          736 t
ORIGIN      
        1 gggcctatcc tggaccctgg cgtcggagat gaagcgcttc gtctcctcga cgatggcgtc
       61 ggtcagataa cgggtctctg caatcggctc gccgttgcgg gtgatggcgg acggaccctc
      121 cctgcccatg ttccagatgt gctcctccca gaagagctcg tggcggtgct cgacgatgcc
      181 gggcgcgcct tcgttggcgt aaagcgagaa ggcttcggtg aagccgtact gctgctcgaa
      241 accgaaggcc tgcgggtggt tttccgggcc gtatcccaga tgccacttgc caaagatgcc
      301 ggtggcgtag ccggccgcgt cgagggcgtc cccaaggctg atctcgctgg ccggaagccc
      361 ctgccgcaat cgctgctcag cgttgggata ggtgtcgaga agaaacggct gcatctcgtc
      421 ggtgtcgatg aggtagcgga atcccaggta ctcgccgagg ttgcgcacgt agcgctgcat
      481 gggctgagac tcgaaaccga agcggttctg gtagcgtccc gtgagcagcg ccgctcgaga
      541 cggcgcgcag atggcggcgg tggcgaaggc gctgaggaac tgcaccccgt cccgcgccag
      601 ggcgtcgatg ttgggggtgt gcgcggcggt gtggccgtac gcggacaggt cgtaccgccc
      661 cagatcgtcg gtgacgatta acaggacgtt gggtcgcttg tcgaaagtct ccgccgcgag
      721 cgctgacaga tagctcgtct tgcccgctgc aagttttggg tcggggccga actcccagcg
      781 ttccgagtcg ggaatcggcc atcccagagc gacccccaga gccccgacac caaggaccag
      841 cccgagcgtc agccgttttc tgctcccctt gctcatgacc aatgggcgct atatgtcacc
      901 gaagtcgccg tgacggccct tgccgtccgc gaaccgggca gcgcccttgt gggtctcgcc
      961 ggatcggatg acctccagtc ccagacgcgt ctcctccctg agcgcatctt ccagtggcat
     1021 cgaccactgg cgataggaag aaagccggtc gttgcgcagg cacagctggg gaaattccgt
     1081 gaggctccgg gccagcttca tggccgcttc gagggcttca cccggcggcg cgatgcggtt
     1141 ggccagaccc atggctttcg cctcgtcccc cgagacgccc cggcccgtga ggatcagatc
     1201 cagcgcgtgg ctgtggccga tgagccggga cagccggatg gtgccgccgt ccaccagcgg
     1261 tacgccccag cgccggcagt agacgccgaa gacggcatcg gaggctgcca cccgcatgtc
     1321 gcaccacagt gccagctcca ggccgccggc cacggcgaac ccctcgaccg ccgcgatgac
     1381 gggcttggag agcagcatgc gcgtgcatcc gaggggcccg ttgccggtct cgcgcaatcg
     1441 gggcccggcg ccggcggcga cggctttcag atctgcgccc gcgcagaaaa cgccatcgcc
     1501 tccggtgagc acggcgacgt ctgacgcgtc gttcgcgtcg aatgccgaaa acgcctcggc
     1561 cagggcgtcg gcggtgggac cgtccacggc gttgcgaact tcggggcggt cgatggtgac
     1621 cacggtgacg cggccgacct gtctaacgtc tacggtcatc actctccccc tttccggcgg
     1681 cttcaggcca cgtcgaattc caggggcagg ctccagggct cgatgcggat ggcgatgggc
     1741 gtgggtttcg agccggtctt gaaacggggg ttgcggatga cctccatgag catgcgggtg
     1801 ccgatgacgg tctccgcccg cgccagctga tagcccacgc agaaatgctt gccgaggcca
     1861 aagccgagat gcccggccat gccctgctgg tggtagccgg agcgcagctc cttgccgaag
     1921 tagagatcct cacgaaacag atcgaaacga tccggatccc tgaacactcg ctcgtcccgg
     1981 ttggccccgt agatggacag ctgcacgtac gcgccggcgg ggatcacctt gccgtggagc
     2041 tccacgtccc gtttcgcccg tcgcgcctgg gcgcttgccg ggggatgaat gcgcatcatc
     2101 tccgagaagg cctggtccag cagatcgggg tctgcctgca cggccttcca ctgctccggg
     2161 ttggccagca gctggtacca gagattggcg atggccttgt ccgtggtctc cgcgccggcc
     2221 accaggatca ggctggcgaa ggacttgatc tcttccttgg tcatgcgctg gccgtccacc
     2281 accgcaacgg ctaggcgcga gagcaggtcg tcggcggggt tgcgactacg ggcatcgatg
     2341 agcggatcta agtaggcgtg atactcggcg ttggccgcca cgccgagagc ccgcagctcc
     2401 ggccccccgt tgatgccgtt catcatggcc ggataccact ggtgaaacat ctcgtgggct
     2461 tcctggggga ggtcgaggac gtcgaggatg acgttcagcg gcagccgggt ggcaaagtcg
     2521 tcgacgatgt cgatctcgcc cacggcggcg gcctggcccg cgatccggcg ggcgtgcaac
     2581 tcggtgaagg cattgatgag agccatggcg ttgcgcccga cggtgggcag cagggccttg
     2641 agcttcttgc cgacgaactg cggcgcgacg atgttgcgca gcttggtgtg gtcgtcgccg
     2701 tcgtactcca tgagcgttgg gccaaacacc gcgccgattg attccccgtt gggagatgcg
     2761 gaaaagttct cggtatcccg gaagccgttc accacgtcct cgtagcgggt gatgatccag
     2821 tgatcggtgc ctggaacgtt atagaccggg tactgatcgc gcagcagctt cagcgtcggg
     2881 tagggatccg ccgcgtactc cgcgctggcg aagacggctg gatcgatggt tggccggttt
     2941 ccgctcatgg tggcgtcctg ttcatcgcaa gattcgatta ggattgatga ttgcacaaac
     3001 agcgggaggc tgccccagga cctgttcgag catgcgcaga aaaacgacga agctgctgtt
     3061 catcactgga ggttggttga gcctcgctct cggcatcgcc gggatcccgc tgccgttgct
     3121 gcccaccacg cctttcctgc tgctggccgc cttctgcttc gccgaaggat ccgatcgtct
     3181 gcacgactgg ctcgtcaatc acgttcactt cggaccgccc atccggcgct ggcgcgaaca
     3241 ccgcgcggtt tcccgccgaa ccaaatggat gggcagcttg agtctgctgg ccgttctggc
     3301 cgtgagcctc gggttcggtg cgccgctgtg gctgctggcg gtgcagggat gcgtcgtggt
     3361 tggcggcggg gcgttcctgt ggacccttcc ggagccgccg gcagaggagc agtagagcag
     3421 gggccggggg acggagcggg cgttctgccc gctccgagcc cggttggctc aatcgccgag
     3481 catcaccgcc agggtcgcgc cgtactggcg cggctgggtg ggggcggcga atgcgaacag
     3541 cgtggccacg ggcgtaccgc ccaggcgctc gatgttgtcc gacaggttct tgccccacac
     3601 cgagaaccgg tagcggcggt tggccgactc ccaaacggcc tgggcgtcga tggtggtcag
     3661 ctcgtcccgg tagaagccca cgtcgttggg cgtggtgagc accatgtcgt ccgtgtagga
     3721 gaaccgggcg ccgatggtca ggttgcccat gtcggccagg tcgaactcgt agctcgcacc
     3781 gatgctcata tcccacttgg gcgcccgcac cagatcgaga tcgctgttgt cggtctccac
     3841 gccgtcgccc gtaaggtcag cgacgaagcc cttgtactcg atgtccaggt agccgatgtt
     3901 gccattgatg ttcagcccgg ccacggggac gaagctgaag ctgagttcgg ccccgaaaga
     3961 ctccgccccc gcggcgttgt ccacgaaggt ctcctggccg gtggaggtgt tcgcctcccg
     4021 gatcacgccc cgctgcaggt ccttgtactc ggcgaa
//
LOCUS       DBNHLM010000266        13778 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_98954, whole genome shotgun sequence.
ACCESSION   DBNHLM010000266 DBNHLM010000000
VERSION     DBNHLM010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13778)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13778)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13778
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_98954"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="AC16QF_12385"
     CDS             complement(<1..824)
                     /locus_tag="AC16QF_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014869324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6449403.1"
                     /translation="MTDQSYDSRNIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVQEL
                     VDNAIDEALAGHCDVIDVVVHPGEAVTVRDNGRGIPVDVHPEEGVSAAEVIMTTLHAG
                     GKFDDNSYKVSGGLHGVGVSVVNALSEEFRLTVRRDGRVYEQIYNDGVPAAPLKEVGE
                     TERRGTECFFKPSDKTFSNIEFHYEVLAKRLRELAFLNSGVHINLQDERTGKQESFVY
                     EGGLRAFVEYLNQKKNPLNEVFHFSVEREDGIGVEVAMQWNDSFQEQVFAYTNNIP"
     gene            complement(817..1911)
                     /gene="recF"
                     /locus_tag="AC16QF_12390"
     CDS             complement(817..1911)
                     /gene="recF"
                     /locus_tag="AC16QF_12390"
                     /inference="COORDINATES: protein motif:HMM:NF014516.7"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR6449404.1"
                     /translation="MHFERLEISHFRILDDVALDLNPGLNFFHGDNGAGKTAILEAAH
                     LLVRGRSFRSHRPRELIQQGQEQLTVRARVADGAMGQRTIAMGRDLSGRTELKINGMQ
                     ERRLSEAARLIPLQIMLPNVADLVLGGPGVRRQWLDWGVFHVKPDYLRTQREYVRLLR
                     QRNAALKAAYVSSPEFEAWTAQLVQAADRVDAQRKSHIAEVGPLFRATLARLAPDLEV
                     ELIYRRGWPSGETLDKVLGEWAPREVKLGSTQAGPHRADVELRVGDARAAGLLSRGQA
                     KAVASAMMVSQAQLLADGLKQTSVFLIDDVGAELDEGHNARFFQLLDDMDSQILATST
                     RLPLGGSCFAGERMTVFHVEHGRAQRSLND"
     gene            complement(2011..3114)
                     /gene="dnaN"
                     /locus_tag="AC16QF_12395"
     CDS             complement(2011..3114)
                     /gene="dnaN"
                     /locus_tag="AC16QF_12395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR6449405.1"
                     /translation="MKFTTNREALLRPLQLVTGVVERRQTLPVLANLLVRARDGVLSM
                     TGTDLEVELVAESSDLEIERDGEATIPARKLADIWRSLPEGAEVTVSVEGDRANVRSG
                     RSRFSLATLPAADFPKVESGPGDLELKLDQSSLRSLLDQVSFSMAQQDVRYFLNGMLL
                     EVTTEHTRSVATDGHRLAMCTLPGGAAGVDRVQAIVPRKGVLELSRLIDDGDDEVVVQ
                     MGANHLRVTQGPYSLTTKLVDGKFPDYEKVIPRDAARSVTGDRETLKQAFTRASILSN
                     EKYRGVRLIVEPEQLTIQANNPEQEEAEEIVPVDYEGERLEIGFNVSYLQDVLNVLDT
                     DEIKISVSDANSSALVEGTGNENAVFVVMPMRL"
     gene            complement(3134..4459)
                     /gene="dnaA"
                     /locus_tag="AC16QF_12400"
     CDS             complement(3134..4459)
                     /gene="dnaA"
                     /locus_tag="AC16QF_12400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR6449406.1"
                     /translation="MGRSVWHKCLDRLQDELSAQQFNTWIRPLQARQHDSALVLMAPN
                     RYVKNQVQVAYLDRITELLDQLGDPLSPLEVKVDVGIADEGESRPLRADGSGGARIQP
                     VHQLNGEFRFETFVEGKSNELAKAAALQVAANAGKSYNPLLLYGGVGLGKTHLMQAVG
                     NCVLDENPDAKVVYLHSQRFVQDMVKALQQGTMQDFMQYYRSVDTLLIDDIQFFAKKL
                     RSQEEFFHVFNGLLERGHQMVLTCDRYPREIDGLEERLQSRFVWGLTVEVEPPELETR
                     VAILMKKAEAEQVDLDPAVAFFIGERIRSNVRELEGALRRVIANARFTGTRITIEQVK
                     RALRDLLAIQDRQVSIDNIQRTVAEYYNIKISDILSKRRNRSIARPRQMAMCLAKELT
                     NHSLPEIGEAFGGRDHTTVLHACRKIAELRESSADVAEDFKNLNRVLSS"
     gene            5014..5148
                     /gene="rpmH"
                     /locus_tag="AC16QF_12405"
     CDS             5014..5148
                     /gene="rpmH"
                     /locus_tag="AC16QF_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR6449407.1"
                     /translation="MKRTFQPSNLKRKRTHGFRSRMSTRGGRQVINARRAKGRKRLSV
                     "
     gene            5090..5512
                     /gene="rnpA"
                     /locus_tag="AC16QF_12410"
     CDS             5090..5512
                     /gene="rnpA"
                     /locus_tag="AC16QF_12410"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR6449408.1"
                     /translation="MAARSSTRGAPKVANASPSEAGDRAAASIHSFPRRRRLTRSRDF
                     DRVLKNAEVRRRSGPLRLTAVANTMQGARLGMIVGKRAVGRAHERNRMKRVIRDEFRR
                     RHAELADMDMVILVMAELENRELRAHLARLFESEVQKR"
     gene            5509..5751
                     /gene="yidD"
                     /locus_tag="AC16QF_12415"
     CDS             5509..5751
                     /gene="yidD"
                     /locus_tag="AC16QF_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR6449409.1"
                     /translation="MMLRNAPRRLLQAGIRVYQWTLSPYLGRQCRFYPTCSHYAHEAI
                     EEFGAVRGSVLAARRLGRCHPFHPGGVDLVPTQEEG"
     gene            5758..7488
                     /gene="yidC"
                     /locus_tag="AC16QF_12420"
     CDS             5758..7488
                     /gene="yidC"
                     /locus_tag="AC16QF_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIR6449410.1"
                     /translation="MLPTEVLRIVLLIALGVVGYLLILQWNEDYMQRPAAPDYAAEPS
                     PSAAEPALTAAPDLPVGAELPAGTGPSDIPDESLIGQDGAETQPALSVPMPDIGERLV
                     VVRTPLLHVWIDRLGGDVVRVALPKFPVDLDRPDVPFVLLDNGLDRTYVAQSGLIGPD
                     GIDAGGDRPLYATQAQSYTLEAGELVVELSTLRDGLEVAKRYMFRSDDYLMEVSYQVR
                     NLTGRTISARMFGQLKRDRKEPYVDTSIALGPQPYLGAAFTTAENRYEKLDFDDLDEG
                     RFRTTEQGGWVAFLQHYFLSAWIANPEETNSYYGQRRPDGTYMFGFSSPVSQVASGAL
                     GEWAASLYVGPKDQKRLEEIAENLNLTVDYGFLWWLAVPLFYLLDWLHGFVGNWGVAI
                     ILLTLLVKLVLYPLSATSYKSMANMRRVAPQMKRLQERHADDRQKLSQEMMALYKKEK
                     VNPLGGCLPMLLPMPIFIALYWVLFESVELRQAPFALWINDLAAMDKYFVLPLLMGAS
                     MYLQQLLSPAVGDPMQVRMMRLMPIMFTVLFLFFPAGLVLYWLVNSVLSIAQQWYVIR
                     QTERAYAAKS"
     gene            7466..8836
                     /gene="mnmE"
                     /locus_tag="AC16QF_12425"
     CDS             7466..8836
                     /gene="mnmE"
                     /locus_tag="AC16QF_12425"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007160130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR6449411.1"
                     /translation="MPTPPKADSLGLDVDTIAAVATPVGSGGVGIVRISGPRARAIGE
                     TVSARSLHPREAFYCRFRNAQGEVVDDGIVLYFAGPASFTGEDVVELQGHGGPVVVQM
                     VLGIVLEAGARQARPGEFSERAYLNGKMDLAQAEAIADLISSSSTAAARGAVRSLQGV
                     FSERVRKIDEAVLGVRMYVEAAIDFPEEEVDFLSEGDIAARITAILADVDDLIRDSRQ
                     GVLLRDGVTIALLGAPNVGKSSLLNALAGEDRAIVTDIPGTTRDLVRADLELAGIPVT
                     VVDTAGLRTTDDPVEREGVRRAEDQARSADLILVVTDVTDPNECSIELPEASQEIPVI
                     QVHNKIDRTSAPPGPLTGEPLPVVRVSALTGDGIDELRAQILAAVGYTEHAGTFTARQ
                     RHLLALQAAAAGLARAAGLAASADGGELVAEELRGVHTELGTLLGGITADDLLGEIFS
                     SFCIGK"
     gene            8910..10847
                     /gene="mnmG"
                     /locus_tag="AC16QF_12430"
     CDS             8910..10847
                     /gene="mnmG"
                     /locus_tag="AC16QF_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR6449412.1"
                     /translation="MQFPESFDVIVIGAGHAGAEAAAAAARVGARTLLLTQSIETIGQ
                     MSCNPAIGGIGKSHLVREIDALDGIMAKAADRAGIQFRILNSSKGPAVRATRAQADRA
                     LYRQAIRELLEAQENLYLFQQSVVDLIVEDGSVRGVVTQIGLEFRAPCVVLTTGTFLG
                     GRIHIGLDNHPGGRAGDAPSNALAERLRALPFRVDRLKTGTPPRIDARSVDFSVMAEQ
                     PGDEPIPCLSFLGSPDDHPRQVSCHVTHTNDRTHSLIRAALDRSPLYTGVIEGVGPRY
                     CPSIEDKVVRFSERTEHQIFVEPEGLDTHELYPNGISTSLPFDVQVALVRSIAGFERA
                     HITRPGYAIEYDFFDPRDLHYSLETKALEGLFFAGQINGTTGYEEAAAQGLLAGTNAA
                     LKVAGRAPWCPRRDEAYLGVLVDDLVNMGTKEPYRMFTSRAEFRLRLREDNADHRLTP
                     VGRDLGLVGEKRWDAYTTKRERVERVHGRMATRNVIAGSEVADRLASLTGERMTRDQT
                     LITYLKRPGVGIAALVGLLGDVLDLQDGDLDGQVLAQVEVEAKYEGYIARQEEEIARI
                     RRHEGLKLDEGLDFAAIDGLSAELKQKLTEARPSTLARAARIPGMTAAGLSVLLVHAR
                     RARATGAVAAPEDEPARGSGG"
     gene            10831..11475
                     /gene="rsmG"
                     /locus_tag="AC16QF_12435"
     CDS             10831..11475
                     /gene="rsmG"
                     /locus_tag="AC16QF_12435"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR6449413.1"
                     /translation="MVPVAEAIAEELRGGAAELGMTLSACQVEKLVAYADLLRRWNDK
                     FNLISRRDVPRLVSRHLLDSLSISPWLGGETTLDLGSGAGLPGIPLAIAHPEREFLLA
                     DRSARKARFVEQAAMRLGLSNVSVFAGDAAALEQSFACVVARAVAGPAELWRIAGHLV
                     RPGGRLVCMSRTAGSGEGATLAADVPDAGRVLAERVAVPGNAGPHEVIVIEAAA"
     gene            11472..12248
                     /locus_tag="AC16QF_12440"
     CDS             11472..12248
                     /locus_tag="AC16QF_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR6449414.1"
                     /translation="MTRIIAVANQKGGVGKTTTSVNLSASLALSGSRVLLIDLDPQGN
                     ATMGSGVEKGGLERSIYHWLMEEVPLDDVLQRCASGYALLPSNTDLTAAEVELLSQPQ
                     REHRMRRRLAEDAARFDFVIIDCPPSLNILTLNALIAADGVLIPMQCEYYALEGLTAL
                     LETVEGVRQQANPSLSIEGLLRTMYDPRNALTRDVSRQLIQYFGDRVFRTVIPRNVRL
                     AEAPSHGLPAILYDRQSRGAVAYMALAGEIKRKHEEDDDP"
     gene            12253..13137
                     /locus_tag="AC16QF_12445"
     CDS             12253..13137
                     /locus_tag="AC16QF_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009402535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR6449415.1"
                     /translation="MRVTKKRLGRGLDALLSGEVVADLGAGQKGPGIPEGVQVAEIGL
                     DQVVPNRYQPRRDFNEEALAELVASIRSQGVMQPVVVRRRAQGGYELVAGERRWRAAG
                     LAGLDAIPALIRDVSDEQASAMALIENIQREDLNPLEEAHALQRLRDEFGLTQQQVAD
                     AVGKSRVAVTNLLRLLNLGPAVRDLLVAGAIEMGHARALLSLDSVVQERAANDVVARG
                     LSVRQTEALVRRLQQGKGPGRPSEAVRDADTAHLERELSEKIGAPVSISCNAKGRGRL
                     VIRYGSLEELDGILGHIR"
     gene            13283..13621
                     /locus_tag="AC16QF_12450"
     CDS             13283..13621
                     /locus_tag="AC16QF_12450"
                     /inference="COORDINATES: protein motif:HMM:NF015834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HIR6449416.1"
                     /translation="MPYRTVALQALAGLGVVLAALAWDAREAVAALAATIVSVVPNAY
                     FAWRVSRERAAARILAAEVVKVVATVGMMGLAFATMELRPLGFFATFGVLQLAHVVGG
                     TSLAGKATSR"
BASE COUNT         2487 a         4318 c         4332 g         2641 t
ORIGIN      
        1 tgcgggatgt tgttggtgta cgcgaacacc tgctcctgaa agctgtcgtt ccactgcatg
       61 gctacttcta cgccgatgcc gtcttcccgt tcgaccgaga agtgaaagac ctcattgagc
      121 gggttctttt tctggttgag gtactccacg aaagcccgca ggcccccctc gtagacgaaa
      181 gactcctgtt ttcccgtgcg ctcgtcctga aggttgatgt gaacgcccga gttgaggaag
      241 gcgagttccc gcagccgctt ggccaatacc tcgtagtgaa actctatgtt gctgaaggtc
      301 ttatcagagg gcttgaagaa gcactccgta ccgcgacgct ccgtctcgcc gacctctttc
      361 aacggtgccg ctggcacccc gtcgttgtag atctgctcgt aaacgcgccc atcgcggcga
      421 acggtgaggc ggaattcctc tgaaagcgcg ttgacgaccg aaacgccgac cccgtgcagg
      481 ccgccagaaa ccttgtagct attgtcatcg aatttgccgc cggcgtgcag cgtggtcatg
      541 attacctcgg ctgcggacac gccttcttcc gggtggacgt ccacgggtat gccccgaccg
      601 ttgtcgcgca ccgttaccgc ttctcctggg tggaccacca cgtcgatgac gtcgcaatgg
      661 ccggccaggg cttcatcgat ggcgttgtcc accaactcct gaaccatgtg atgcaggccg
      721 gtgccgtcat cggtatcacc gatgtacatt cccgggcgct tgcggacggc gtcaaggcct
      781 ttgagcacct tgatgttgcg tgaatcgtat gattgatcag tcattcaggc tcctttgcgc
      841 gcgaccgtgt tccacgtgaa acaccgtcat cctctctccg gcgaagcaac tgccacctag
      901 aggcagacgg gtggacgtag ccagaatctg gctatccatg tcgtcgagca attgaaaaaa
      961 acgggcgtta tgaccttcgt ccaactcggc gcccacgtca tctatcagaa atacgctggt
     1021 ctgcttgagc ccatcggcca gcagctgagc ctggctgacc atcatggcac tggcgaccgc
     1081 ttttgcctga ccccgggaca gcagccccgc ggctcgagca tcacctaccc gcaactccac
     1141 gtccgcccga tggggccctg cctgcgtaga acccaatttt acctctctcg gcgcccattc
     1201 gcccaacact ttgtccaagg tttctccgga gggccaacct ctgcgataga tgagctcgac
     1261 ctccaagtcc ggggcaagtc gggcaagagt ggcacgaaac agcgggccaa cctctgcaat
     1321 gtggcttttc cgctgggcgt ctacccggtc tgccgcctgg acgagctgcg cggtccaggc
     1381 ctcgaactcc ggcgaggaaa cgtaggcggc cttcaacgcc gcgttacgtt gccgaagtag
     1441 tcgaacgtac tcccgctgcg tcctgaggta atcaggtttc acgtgaaaca cgccccagtc
     1501 cagccactgg cggcgaactc cagggccgcc aagcacgaga tctgccacgt ttggcaacat
     1561 gatctgcagc ggtatgagcc gcgcggcctc ggagagccgg cgttcctgca tcccgttgat
     1621 cttcagctcg gtacgcccgg acaggtcccg ccccatggcg atggtgcgct ggcccatggc
     1681 tccatccgcc accctcgccc tgacggtgag ctgctcttgg ccctgctgaa tcagctccct
     1741 ggggcggtgg cttcgaaaag accgcccgcg gaccaggaga tgcgcggcct cgagaatggc
     1801 cgttttgccc gcgccgttat ctccgtgaaa gaagttgaga cccggattca gatccagagc
     1861 cacatcatcc agtatccgaa aatgggaaat ttctaaccgt tcgaaatgca cgttcgggcg
     1921 ggttcgacga acatcattgc attatccgct tttgcagcgc aggcgtcccc tgtccacggc
     1981 gtgcaggcgc gggtcacccg cgccttgggg ctagagccgc atgggcatga cgacgaagac
     2041 cgcgttctcg ttgccggtgc cttcaacgag agcgctgctg ttcgcgtcgg atacgctgat
     2101 cttgatctcg tccgtatcca gcacgtttag cacgtcctgg aggtagctga cgttgaaacc
     2161 gatctccagg cgctctccct cgtaatccac cggaacgatc tcctccgcct cttcctgctc
     2221 cgggttattc gcctgaatgg tcagctgttc cggctcgacg atcaggcgca cgccacgata
     2281 cttctcgttg gaaagaatgg aagcacgcgt gaatgcctgt ttgagcgttt cgcgatcgcc
     2341 ggtgaccgac cgggccgcat ctcgaggaat gaccttctcg tagtccggaa acttgccgtc
     2401 caccagcttg gtcgtcagag agtagggccc ctgggtgacc cgcaggtgat tcgcgcccat
     2461 ctggaccacg acctcgtcat ccccatcgtc gatgaggcgg ctgagttcga gcacaccctt
     2521 tcggggaacg atggcctgca cccgatccac acccgccgct ccgccgggca gcgtgcacat
     2581 ggccagcctg tgaccgtccg ttgccacgga gcgtgtgtgt tccgtggtga cctccagcaa
     2641 cattccgtta agaaagtagc gtacgtcctg ctgggccatg gagaaactca cctgatctag
     2701 caggcttcga agactggact gatcgagctt cagttccagg tcgccagggc cgctttccac
     2761 tttgggaaaa tctgctgctg gcagggtggc tagagaaaaa cggctgcggc cggagcgcac
     2821 gttggcgcgg tcgccttcca ccgaaaccgt aacctcggcg ccctcgggga gcgatcgcca
     2881 tatgtccgcc agcttccggg cggggatggt agcctctcca tcccgctcga tctcaagatc
     2941 tgacgactcc gctaccagct cgacctcgag gtccgtgccg gtcatcgaca gcacgccgtc
     3001 ccgggcgcgg actaacaagt ttgccaacac gggcagggtc tggcgccgtt caacgacccc
     3061 cgtcaccaac tgcagcggtc gaagcagggc ttcccgattg gtcgtgaatt tcatgtcttc
     3121 ctacgatgtg aaatcagctg gacaacacgc ggttgaggtt cttgaagtcc tcagccacgt
     3181 cggcgctact ttcccgaagc tccgcgatct tgcggcaggc gtgaagaacc gtcgtatgat
     3241 ccctgccgcc gaacgcttca ccgatctccg gcagagaatg gttggtcagc tccttggcaa
     3301 gacacattgc catctgccgg ggtcgggcta tggaccggtt gcgccgcttg gacagaatat
     3361 ccgaaatctt gatgttgtaa tactcagcga cggtgcgctg aatgttgtcg atgctgacct
     3421 gccgatcctg aatcgcgagc agatcccgca acgcccgctt gacctgctcg atggtgatcc
     3481 tggttccggt gaaacgcgca ttggctatca ctctgcgcaa cgctccttcg agctcccgca
     3541 cgtttgacct gatccgctcg ccgatgaaga acgccaccgc cgggtccaga tcgacctgct
     3601 ccgcttctgc cttcttcatc agaatggcta cccgggtttc gagctcgggc ggctccacct
     3661 cgacggtcag accccaaacg aaccgcgatt gcagccgctc ctctagacca tcgatctccc
     3721 gcggatagcg gtcgcaggtg agaaccatct gatggccacg ctcgagcagg ccgttgaaaa
     3781 cgtgaaagaa ttcctcctgg gaacgcagct tcttcgcaaa gaactgaatg tcgtcgatga
     3841 gcagggtatc caccgaacgg tagtactgca tgaagtcctg catggtgccc tgctggagcg
     3901 ctttgaccat gtcctgaacg aagcgctgag agtgaagata gaccaccttg gcatcgggat
     3961 tttcatccag cacacagttg ccgaccgcct gcatgagatg cgtcttgccc aagcccaccc
     4021 cgccatagag cagcaacggg ttgtacgact tgccggcgtt ggccgcaacc tgcagggcgg
     4081 cggccttggc cagctcgttt gacttgccct cgacgaacgt ctcgaaacga aactcgccgt
     4141 ttaactgatg aaccggctga atgcgagcgc cgccgctgcc gtcggcacga agcggtcggg
     4201 actcgccctc atcggctatg cccacgtcca ccttgacctc gagcgggctc aacggatctc
     4261 ccagctgatc gagcagctcg gttattcgat caagataggc gacctgcacc tggttcttca
     4321 catagcgatt gggcgccatg agaaccaggg cgctgtcgtg ctgacgtgcc tgcagaggtc
     4381 ttatccaagt gttgaactgc tgagcggaaa gctcgtcctg caaccgatca aggcacttat
     4441 gccaaacgga ccgtcccacc aaatgccccc gtatgacccg ttctcttggc agagaatctg
     4501 tagttttgat gtgcgcgaac tgcgaagcat tctagccagt aaacgaaagc atttgccatg
     4561 caataggaag catttccggc aggttttttg gtatcgactg caaattcaga aacttacgtc
     4621 tgccgttggc catggtgagc tcgcgattcg agcttgcggc gatgtgagca actcgttgaa
     4681 gaacctgtgg atagttttcc gccgctcgct ttacccccga tgatcccgcc cgggtcctca
     4741 acgggcccca agcatatcca cacccttgca gcgaggaaaa caacttgccg gggcaaagac
     4801 ttacgcccga tatcaacaga tgctgccgtc tcaacaagca acaactacaa attatctata
     4861 tatatttctt actatgagaa ggagtgatgt cgcagctgcg ttgacactca aggggcctga
     4921 ttcataaaat cccgctccct cgcgatccag gccgccgact aaagcgcgcc aaaggggcgg
     4981 gagcttcggt aagtcagcaa cggaaacccg aacatgaaga gaacgtttca gccaagcaat
     5041 ctaaagcgca agcgcactca cggcttccgt tcgcgtatgt ccactcgagg tggccgccag
     5101 gtcatcaacg cgaggcgcgc caaaggtcgc aaacgcctct ccgtctgagg caggcgatcg
     5161 cgcagctgct tcgatccact catttccgcg aaggcgccgg ctaaccagat cgcgggattt
     5221 cgaccgcgtc ctgaagaacg ccgaggtgcg ccgccggagc gggccgttac ggctgacggc
     5281 ggtagcgaat acaatgcagg gcgcccgcct ggggatgata gtcggcaagc gggccgtcgg
     5341 cagggcccac gaacgcaacc gaatgaagcg cgtgatcagg gacgagtttc gtcgacgcca
     5401 cgcagagctg gccgacatgg acatggtcat tctggtgatg gctgagctgg agaaccgaga
     5461 gcttcgcgcc catcttgcga ggctgtttga gtcggaggtg caaaaaagat gatgcttcgc
     5521 aacgcaccgc gccgcttgct tcaggcgggc atccgtgtgt atcagtggac gttgagtccg
     5581 tacctggggc gccagtgccg cttctacccc acctgctctc actacgccca cgaggccatt
     5641 gaagagttcg gcgccgtccg gggctccgtc cttgccgcgc gaaggctggg gcgctgccac
     5701 ccgtttcatc ccggcggcgt cgatctggtt ccgacccaag aggagggttg atcagccatg
     5761 ctgccaacgg aagtcctcag gatcgttctc ctcatcgcgc tgggggtggt gggttacctg
     5821 ctcatcctgc agtggaacga ggattacatg cagcgtcctg ctgctcccga ctacgcggcc
     5881 gaaccctcgc catccgctgc ggagcctgcg ctgacggccg cgccggattt gcctgttggc
     5941 gcggaactcc ctgcgggtac gggaccatcc gacatcccgg acgaatcgtt gatcggtcaa
     6001 gacggtgctg aaacccagcc ggccctcagc gtgccgatgc cggacatcgg ggaacggctc
     6061 gtcgtcgtca ggacgccgtt gctgcacgtc tggatcgacc ggctgggcgg ggacgtggtg
     6121 cgcgtcgcgt tgcccaagtt tcccgtggac ctggatcggc ccgacgtccc gttcgtgctg
     6181 ctcgacaacg ggctcgatcg cacctatgtg gcgcagagcg ggctcatcgg cccggacggc
     6241 atcgatgcgg gcggcgatcg accgctgtat gccacccagg ctcagagcta caccctggag
     6301 gcgggggagc tcgtagtgga gctctcgacg ctccgggacg gtctcgaggt cgccaagcgc
     6361 tacatgttca gaagcgacga ctacctcatg gaagtgtcct accaggttag aaacctgacc
     6421 gggcgcacca tctcggctcg gatgttcggc cagctcaaac gggatcgcaa agagccgtac
     6481 gtggatacct cgattgctct cggaccccag ccctatctgg gggctgcgtt caccaccgca
     6541 gagaaccgct acgagaagct cgatttcgac gatctcgacg aaggccgctt ccggaccacc
     6601 gagcagggcg gttgggtggc cttcctgcag cactacttct tgagcgcctg gatagccaac
     6661 ccggaggaaa ccaacagtta ctacggtcaa cgccgacccg atggcaccta catgttcgga
     6721 ttttccagcc cggttagcca ggtggccagc ggggccttgg gcgagtgggc ggccagcctg
     6781 tatgtgggcc ccaaggatca gaagcgcctg gaagagatcg ctgaaaatct taatctcacc
     6841 gtggactacg ggtttctctg gtggttggca gtccctctct tctacctcct cgactggctg
     6901 cacggtttcg tcgggaactg gggagtcgcc atcattctgc tcaccctgct ggtcaagctg
     6961 gtgctctacc cgctctcggc aacgagctac aagtccatgg cgaacatgcg gcgcgttgcc
     7021 cctcagatga aacggttgca ggagaggcat gcggacgacc gtcagaagct gtcccaggag
     7081 atgatggccc tatataagaa agagaaggtc aacccgttgg gtggatgcct tcccatgctc
     7141 ctgcccatgc ccatattcat cgctctgtac tgggtgcttt tcgagagtgt ggagctcaga
     7201 caggcgccct ttgccctctg gatcaacgac ctcgcggcca tggacaagta tttcgtcctg
     7261 cctcttctga tgggcgcgag catgtatctg cagcagctgc tgagccccgc ggtcggtgat
     7321 cccatgcagg tccgcatgat gcggctcatg cccatcatgt tcaccgtgct gtttctcttc
     7381 ttcccggccg ggctggtgct ctactggctc gtcaacagcg tgctgtccat cgcccagcag
     7441 tggtacgtca taaggcagac ggagcgtgcc tacgccgcca aaagctgact ctctcggcct
     7501 ggacgtcgac acgatcgcgg ccgtggcgac gcctgtcggt tccgggggcg tgggcatcgt
     7561 gcgaatctcc gggccccgag cccgcgccat cggagagacg gtctcggccc gtagcctcca
     7621 cccacgcgag gccttctatt gcaggttccg aaacgcccag ggtgaggtcg tcgatgacgg
     7681 catcgttctc tactttgccg gaccggcctc ctttacagga gaggacgtgg tggagctgca
     7741 ggggcacggc gggccggtag tcgtgcagat ggtccttggt atcgtgctcg aggcaggggc
     7801 ccggcaggca cgccccgggg agttctccga acgggcctat ctgaacggca agatggatct
     7861 tgctcaggcc gaggccattg ccgatctgat ttccagctcg tccacggcag ccgctcgcgg
     7921 ggccgtgcgc agcctccagg gcgttttctc cgagagggtc cggaagatag atgaagcggt
     7981 actcggtgtg cggatgtacg tggaagcggc catcgacttt ccggaggaag aagtggattt
     8041 tctcagcgaa ggcgacattg ctgcccggat cacggccatc ctggctgacg tcgacgatct
     8101 gatccgggat tcccggcagg gcgttctgct tcgggacgga gtcaccatag cgcttctcgg
     8161 cgcgcccaac gtgggtaagt cgagtctgct gaacgcgctg gccggagagg accgggccat
     8221 cgtgaccgac attcccggga ccacgcgcga tctcgtgcgc gccgacctcg agctcgcagg
     8281 cataccggtc acggtggtgg acacggcggg tttgcgaacc accgatgacc cggtggaacg
     8341 cgaaggcgtt cggcgggcag aggatcaggc gcgctcggca gatctgatcc tggtggtcac
     8401 cgacgttacc gacccgaacg aatgttcgat tgagctgccc gaagcgtcgc aggagatccc
     8461 cgtcattcag gtgcacaaca agattgaccg gacaagcgcg ccacctgggc cattgacggg
     8521 cgaacccctg cccgtggtca gggtttctgc gctcaccgga gacggtatcg acgagcttcg
     8581 cgcccagatt ctcgctgccg tcggctacac cgagcacgcg ggaaccttca ctgcccggca
     8641 aaggcacctg ctggcgctgc aagctgctgc tgcgggcctg gcgcgagccg ctggtctggc
     8701 tgcttcggcc gacgggggcg agctcgtggc ggaagagctt cgcggcgtgc acaccgaact
     8761 cggcactctg ctgggcggca ttacggccga cgacctgctc ggcgaaattt tctcgagctt
     8821 ctgcatcggc aagtagcccc ggccgcgccc ttccaatcgg aatccgagta cttatacttc
     8881 gcgggcttgc aacctggcgg atattagctt tgcagtttcc cgagtcgttc gacgtgatcg
     8941 tcattggtgc cggccatgcc ggcgccgaag ctgccgctgc ggcagcccgg gtgggggccc
     9001 ggactctgct gctcacccag agcatcgaaa ccatcgggca gatgtcgtgc aatccggcca
     9061 ttggtggaat aggcaagtcc cacctcgtcc gggagattga cgcgctggac gggatcatgg
     9121 ccaaagccgc cgatcgcgcc ggcatacagt tccgaatcct caactccagc aaggggccag
     9181 ccgtcagggc cacccgcgcc caggcggaca gggcccttta ccgacaagcc atccgggagc
     9241 tgttagaggc ccaggagaac ctttacctct tccagcagtc ggtggtggat ctgattgtcg
     9301 aagacggctc ggtccgtggt gtggtcaccc agatcggcct ggaattccgg gcgccctgtg
     9361 ttgtgctgac gacggggacg tttctcggcg gccgtattca catcggcctg gacaatcacc
     9421 ccggcggccg agcgggagat gcaccgtcca acgcgctggc ggagcgtctg cgcgcgctgc
     9481 cgttccgcgt ggaccggctg aagacgggta cccccccgcg aatcgacgcc cgctccgtgg
     9541 atttctcggt gatggccgag cagcctgggg atgagccgat cccctgcctg tcttttctcg
     9601 gctcccccga cgatcacccc cgtcaggtca gttgccacgt cacccatacc aacgatcgga
     9661 cccactccct tattcgcgcg gcgctggatc gctcacctct ctacaccggg gtcatcgaag
     9721 gcgttgggcc gcgatactgt ccgagtatcg aggacaaggt cgtgcggttc tcggagcgaa
     9781 ctgaacatca gatattcgtc gagccggaag ggctggacac tcacgagctc taccccaacg
     9841 ggatctccac gagcctgccg ttcgatgtgc aggtcgccct ggtgcgcagc atcgccggat
     9901 tcgagcgggc ccacatcacg cggccgggct atgccatcga gtacgacttt ttcgaccccc
     9961 gggacctgca ttactcgctg gagacgaagg cgctggaagg gctgtttttc gccggtcaga
    10021 tcaacggcac caccggatac gaggaggcgg cggctcaggg cctgctcgcc ggaacgaacg
    10081 cggcgctcaa ggtggccggc cgggcgccct ggtgtccccg ccgcgacgag gcctatctgg
    10141 gcgttctggt ggatgatctg gtgaacatgg gcaccaagga gccctatcgg atgttcacca
    10201 gccgggcgga gttccggctg aggcttcgcg aagacaacgc ggaccatcgc ctcaccccgg
    10261 taggccggga tctggggctc gtgggcgaaa agcgctggga tgcgtacacc acgaagcgtg
    10321 agcgggtcga gcgcgtccac gggcggatgg cgacccgcaa cgtaatcgcg gggtcggagg
    10381 tggctgaccg gctcgccagc ctcaccggtg agcggatgac tcgagatcaa acgctcatca
    10441 cctatctcaa gcgtccgggc gtgggcatcg ccgcgttggt cggtctcctt ggggatgttc
    10501 tggacctgca ggatggggac ctcgacggcc aggtgctggc gcaggtggag gtggaagcta
    10561 agtacgaggg ctacattgcg cgccaggaag aagagattgc cagaatccgt cggcacgagg
    10621 gcctcaagct cgacgaaggc ctggacttcg ccgctatcga cgggctttcc gcagagctca
    10681 agcagaagct gaccgaggct cgaccctcga cgcttgctcg cgctgcgcgt atccccggaa
    10741 tgacggcggc cggcctgtcc gttctgctgg tgcacgcccg aagagcccgg gctacaggtg
    10801 ctgtcgccgc cccggaagac gagccggcgc gtggttccgg tggctgaggc gatcgccgag
    10861 gagctgcggg ggggcgccgc cgagctggga atgaccctat cggcctgcca ggtggaaaag
    10921 ctcgttgcct acgccgatct cctgcgccga tggaatgaca agttcaacct gatctcgcgg
    10981 cgggatgttc ctcggttggt atcccgccac ctcctcgaca gtctgagcat ctcgccatgg
    11041 ctgggcggcg agactactct ggacctcggc tcgggggccg gcctgcctgg aatccctctg
    11101 gccatcgccc atccggaacg cgaattcctg ctggctgacc ggtcggcgcg caaggcgcgg
    11161 tttgtcgagc aggcggcgat gcgtctaggc ctttcgaacg tgagcgtgtt tgccggggac
    11221 gccgccgccc tggagcaatc gttcgcttgt gtggttgccc gggcggtggc cggacctgcc
    11281 gagctgtggc gtattgccgg gcacctggtt cgtccggggg gaaggttggt ctgcatgagc
    11341 cggacggcag gctccgggga aggcgcaacc cttgccgccg acgtgcccga cgccggtcgc
    11401 gttctcgccg agcgggtggc agttcctggc aacgcagggc cccacgaggt gatcgtcatc
    11461 gaggccgctg cgtgacgagg atcatcgcgg ttgcgaatca gaaagggggg gtcggcaaga
    11521 ccaccaccag cgtgaacctg agcgcatccc ttgcgctttc cggctcccgg gttcttctca
    11581 tcgacctgga tccccagggc aatgccacca tgggctctgg tgtggaaaag ggtgggcttg
    11641 agcgcagcat ttaccactgg ttgatggagg aggtcccgct ggacgacgtc ctgcagcgat
    11701 gcgccagcgg ctacgccttg ctgccgtcca acacggatct cactgccgcc gaggtggaac
    11761 tgctgagcca gccgcagcgc gagcacagga tgcggcggcg gttggccgag gatgcggcgc
    11821 gcttcgactt cgtcatcatc gactgcccac catcgctgaa catactgacc ctcaacgctc
    11881 tgatcgccgc tgacggcgtg ctcattccca tgcagtgcga gtactacgcc ctcgagggcc
    11941 tgacggctct gctcgaaacg gtcgaaggcg tccggcagca ggccaacccc tcgctgtcca
    12001 tagagggcct gttgcgaacc atgtatgatc cccgcaacgc cctcacgcgg gacgtttccc
    12061 ggcagctgat ccagtatttc ggcgaccggg tgttccgaac agtgatcccg cggaacgtcc
    12121 ggctggcaga ggcgcccagt cacgggcttc cagccattct ctacgatcgg cagtcgcggg
    12181 gcgcggtggc ctacatggcg cttgcggggg aaatcaagcg caagcacgaa gaggacgacg
    12241 acccgtgagc aattgcgcgt gaccaagaag agactgggca gaggtctgga cgcgctgttg
    12301 tcgggcgagg ttgtggccga tctgggcgcg gggcagaagg gccctggaat ccccgaaggc
    12361 gttcaggtcg ccgaaatcgg gctcgaccag gtggtaccga accgctacca gccccgccgg
    12421 gatttcaacg aggaagccct tgccgagctg gtcgcctcca tcagatccca gggagtgatg
    12481 cagcccgttg tggtcagacg acgagcccag ggcggatacg agctcgtcgc cggtgagcgg
    12541 cggtggcgtg ctgccggcct tgcgggcctg gacgccattc cggccctgat tcgtgatgtt
    12601 agcgatgagc aggcttcggc catggcgctc atcgagaaca tccagcgcga ggatctcaac
    12661 ccgctggaag aggcccacgc cctgcagcgg ctgcgagacg agttcggcct cacccagcag
    12721 caggtcgccg atgcggtagg caagtcgcgc gttgcggtga ccaacctgct gcgcctgctg
    12781 aatctggggc ctgcggtgcg ggacctgctg gtggcaggag cgatcgagat gggccatgcg
    12841 cgcgccttgt tgtcgctgga cagcgttgtt caggagcgcg cggccaacga cgtggtcgcc
    12901 cgggggcttt cggttcgcca gaccgaggcg ctggtgaggc gcctgcagca gggcaagggg
    12961 cccgggcgcc cgtccgaggc cgtccgcgat gcagacaccg cgcacctgga acgcgaactc
    13021 tctgagaaga tcggcgcgcc ggtttcgatc tcctgcaatg ccaagggccg aggtcgcctc
    13081 gtcattcggt acggatcgct cgaggagctc gacggtatac tcggtcacat caggtaggcg
    13141 ccggcgccgc tcagatgcgc gtcgttcgaa atggccgctt tgtcaggatc acgctaattg
    13201 aacgcgctct ggcccgtctc tataatacgc gccgcctttc gggggttggt gtttgtcagg
    13261 ctgtcctgac gtggacatgt ctgtgccgta tcgaaccgtg gcgctgcagg cgctcgccgg
    13321 gctcggggtg gtgctggcag cgctggcgtg ggacgcccgt gaagcggtgg cggcgctggc
    13381 cgctacgatc gtcagcgtcg tgccaaacgc ttacttcgcc tggcgcgtca gccgcgagcg
    13441 ggcggcagcc aggatcctcg cggcagaggt cgtgaaggtg gtcgccaccg tgggcatgat
    13501 gggattggcc tttgcgacga tggagttgcg gcctctgggt ttcttcgcca ccttcggcgt
    13561 gctgcagctg gcgcacgtgg ttggcggaac gagcctggcg ggaaaagcga cttcacgctg
    13621 aacgccggcg cgtttgttga cgcgctggtc aaattgccgg ggacgtggtc ttcggcagac
    13681 attggggacg agagcaacgc agcatgtcga gcggaaccgt aacctccggg gagtacatcc
    13741 agcatcacct gacgaacctg acgttcggtc gccatcct
//
LOCUS       DBNHLM010000267         5425 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_995977, whole genome shotgun sequence.
ACCESSION   DBNHLM010000267 DBNHLM010000000
VERSION     DBNHLM010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_995977"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="AC16QF_12455"
     CDS             complement(<1..1290)
                     /locus_tag="AC16QF_12455"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR6449417.1"
                     /translation="MRRRLLRSGYPAPVRQSLAQVGRLIARRRPQLRLQLCALLLPGL
                     VLSGMAVAGPGEKVYKEMLANDQIYQDQEWQAYVNQIGRRLLAVSPDAGKDYHFYILD
                     NPTVNASAFPDAYIFVNRGLIAYLRSEDELAGVIGHEIGHVVGRHARRSNMMGFFGNV
                     AGFIGSIMTGTGAIADMGNTVTQTLRSGYGREFELEADEYGGEFLARAGYNPLAMIDV
                     IHVLKDHSLFAKNVLGQPAVYHGLFSTHPKNDKRLHDAVQKSQHIFPDELREPERDFW
                     EMIDGMVYGDEAATGLIKGETYYHGGLRVVVQFPQGWDVTNTASEVLGRAPGGTPDAF
                     ITVQRLNPAESEQTPEEYVKETLKRDDVTDGESLEISGYSAFIGTVEVASGNAQARRI
                     AVIYKDNGVYLFKGEVGPVGDVNAFEPQWRATLESFRA"
     gene            complement(1455..1670)
                     /gene="rpmE"
                     /locus_tag="AC16QF_12460"
     CDS             complement(1455..1670)
                     /gene="rpmE"
                     /locus_tag="AC16QF_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007350412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR6449418.1"
                     /translation="MKADIHPEYNQIKATCSCGNVIETCSTLEGDIHLDVCSACHPFY
                     TGKQKILDAGGRVERFRKRFGNRSVSS"
     gene            1790..3961
                     /locus_tag="AC16QF_12465"
     CDS             1790..3961
                     /locus_tag="AC16QF_12465"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004067.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HIR6449419.1"
                     /translation="MNPLTQTNVDVVRVAVAKPLHQLFDYAVPPDMTPPVAGARVVVP
                     FGRQRLTAICVERNPDDAHAQLKPLLSVLDDTAAIPEELRALAGWLCDYYQHPLGEVL
                     ATMLPAEARRGHELTIVRPEQWTATGTRSDLARAPRQQALMEALAAAGGTLSASALAE
                     AGFSRALIRAAAGKGLITRTEAATGFTLGIDEQPPELSDEQAGVLSDLLARTDCFAPS
                     LLEGITGSGKTEIYLQLIDNVRKAGHQVLVLVPEIALTPQTVGRFERRFGSGGGVASL
                     QSNLGDQERLQTWLKCRSGEVSVLIGTRSAVFAPFAKLGLIVVDEEHDGSFKQQDGLR
                     YSARDVAVKRAQTLDIPLLLGSATPSLESLNNAMAGRYAHLRLRHRAGGARPPRFHLL
                     DIRGQSLDDGLSQDLLRIVGQHLNRGGQALVFLNRRGYAPTYLCPRCSWQAACGHCEM
                     RLTLHLSPRALLCHHCGHQRPLPSACPDCGHAELIAVGAGTQRTEAGLAKRFPGIPLY
                     RVDRDTTRSQRRMEAQFEAIHKGDPAILVGTQMLAKGHHFPKVTVVAVVNADGGFLSA
                     DYRAPERTAQLITQVAGRAGRAEDPGEVWIQTYQPDGVLLRSLIDEGYDGFARRELAI
                     RKSSGLPPFRPMALLRAESRDAESARNFLTSVRDRLGSPLEVLGPAPAPIARVANRLR
                     FQLMVMADSRRELHAGIRPIRDLTAPGTIRWSVDIDPYDTF"
     gene            4039..4605
                     /locus_tag="AC16QF_12470"
     CDS             4039..4605
                     /locus_tag="AC16QF_12470"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR6449420.1"
                     /translation="MANDFANRLNPPTTRRPKARPPSATRRKERRFSGSSFASGLLIG
                     GACMAVALNLPDPQPERPPAAATAASAPPGSVTFEFDKDLRDAFVEADPGQYETDAGH
                     DGSRPVSYLIQAASFPVAADADSLQAELAQMGLAAQSVPARLNAGTWFRVTVGPFDSQ
                     LKANRALTQLREIDLDAFMIQQRLPEPR"
     gene            4716..5258
                     /gene="hslV"
                     /locus_tag="AC16QF_12475"
     CDS             4716..5258
                     /gene="hslV"
                     /locus_tag="AC16QF_12475"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014754676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HIR6449421.1"
                     /translation="MADIHGTTIVSVRNDRQVAIGGDGQVSMGQTIMKGNARKVRRLH
                     DDKVLAGFAGGTADAFTLFERFEGVLDKYHGNLTRAAVELAKDWRSDRALRRLEALLV
                     VADREATLLVSGTGDVIEPEAGVIAIGSGGPFAQAAARALNENTELDPADIVEKSLSI
                     AADICVYTNHHRTIEVLDLT"
     gene            5255..>5425
                     /gene="hslU"
                     /locus_tag="AC16QF_12480"
     CDS             5255..>5425
                     /gene="hslU"
                     /locus_tag="AC16QF_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576020.1"
                     /note="heat shock protein involved in degradation of
                     misfolded proteins; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HIR6449422.1"
                     /translation="MTQMTPREIVHELDKHIIGQDDAKRAVAIALRNRWRRMQLEEEM
                     REEISPKNILMIG"
BASE COUNT          942 a         1825 c         1746 g          912 t
ORIGIN      
        1 cgcgcgaaac gactccagcg tcgcccgcca ttggggctcg aaggcgttga cgtcgccaac
       61 cgggcccacc tctcccttga agagatatac cccgttgtcc ttgtagatca ccgcgatgcg
      121 tctcgcctgc gcgttgcccg aggccacctc cacggtgccg atgaaggccg agtagccgct
      181 gatctcgagg ctctcgccgt cggtgacgtc gtcccgtttg agggtttctt tgacgtactc
      241 ctccggggtc tgctcggatt cggcagggtt caggcgctgg acggtgatga aggcatcagg
      301 cgtcccgccg ggagctctgc cgagaacttc cgaggccgtg ttcgtgacgt cccatccctg
      361 cgggaactga acgacaaccc gcaacccgcc gtgatagtag gtctcgccct ttatcaaccc
      421 cgtcgccgcc tcgtccccgt agaccatgcc gtcgatcatc tcccagaaat ctctctccgg
      481 ctcgcgcagc tcgtcgggaa agatgtgctg agacttctgc acggcgtcgt gcagccgctt
      541 gtcgttcttc gggtgcgtcg agaacaaacc gtgatagacg gccggctggc cgagcacgtt
      601 cttggcgaac agcgagtggt ccttgaggac gtggatcacg tcgatcatgg ctaacgggtt
      661 gtaaccggca cgggccagaa actctcctcc gtactcgtca gcttcgagct cgaactcgcg
      721 gccatagccg gagcgcagcg tctgggtcac cgtgttgccc atgtctgcga tggcaccggt
      781 tccggtcatg atcgatccga tgaacccggc aacgtttccg aagaaaccca tcatgttgct
      841 gcggcgggcg tggcggccga cgacgtggcc gatctcgtgc ccgatgaccc cggccagctc
      901 gtcctcagac ctgaggtagg cgatgagccc gcggttcacg aatatgtacg cgtcgggaaa
      961 agccgaggcg ttcaccgtgg gattatcgag gatatagaag tggtaatcct tgcccgcgtc
     1021 agggctcacc gcgagcaggc gccggccaat ctggttcacg taggcctgcc actcttggtc
     1081 ctggtagatc tgatcgttgg ccagcatctc cttgtagacc ttctctcccg ggccagccac
     1141 cgccatgccg gagaggacca agccgggcag aagcagcgcg cagagctgca ggcgcagctg
     1201 cgggcgccgt cgcgcgatca gccgacccac ctgcgccagc gattgccgca caggcgccgg
     1261 gtagccgctt cggagtagac gtcttctcat tcgccgggat ccaggaatca cgagcaggtc
     1321 agccgtatct tacgagacgc tgcgaaaacc gccagggaca ccgaacctta gactggagga
     1381 gcatgaaacg gttcaacgcc gtccgcgatc cacggggcgg cgtcagcgct gaggggatgc
     1441 ggtgaggggt gcggtcagga agaaacggag cggttgccga agcgcttgcg gaatcgctcg
     1501 acgcgaccgc cggcgtccag gatcttctgc ttgccggtgt agaacggatg gcaagccgag
     1561 cagacgtcca ggtggatgtc accttccagc gtcgagcagg tctctatgac gtttccacag
     1621 ctgcaagtgg ctttgatttg gttgtattcg ggatggatat ccgctttcac ctgactgctc
     1681 ccttggatca cgttccggga aaagggcgcg aagtttatta agaggcgcgg tgaatggcaa
     1741 cccgcgcgcc ggtcgaagca ttacaatgcc agctcccgag gagagggctg tgaacccgtt
     1801 gacccagacg aacgtggacg tggtgcgcgt ggccgtggca aagcctcttc accagctgtt
     1861 cgactacgcg gtgccgccgg acatgacgcc accggttgcg ggggcgcgtg tcgtcgtgcc
     1921 gttcggcagg cagcgtctta ccgcgatctg cgtggagcgc aaccccgacg acgcccacgc
     1981 ccagctcaag cctctgctca gcgtcctgga cgataccgcc gccattcccg aggagctgcg
     2041 cgccctcgcc gggtggcttt gcgactacta ccagcaccct ctcggcgagg tgctggccac
     2101 catgctgccg gcagaggctc gccgcgggca cgagctgacg atcgtcaggc cggagcaatg
     2161 gacggcgacc ggcaccaggt ccgacctggc gcgagcgccc cgccagcagg cgctcatgga
     2221 agccctcgct gccgcgggcg gcaccctgtc cgcgtcggcg ctcgccgaag cgggtttttc
     2281 ccgggccctc atccgcgctg ccgcaggcaa ggggctgatc acccgaaccg aggccgccac
     2341 gggctttacc ctgggcatcg acgagcagcc gccggagctg agcgacgagc aggccggcgt
     2401 gctctccgac ctgctcgctc gcaccgattg ctttgcgcca tcacttctgg aggggataac
     2461 gggcagcggc aagaccgaaa tctacctgca actgatcgat aacgtgcgga aagctggcca
     2521 ccaggttctc gtgctcgtcc ccgagatcgc gctcacccct cagaccgtcg ggcgcttcga
     2581 acgccgcttc ggatccggcg gcggggtggc cagcctccag tccaacctgg gtgatcagga
     2641 gaggttacag acctggttga agtgccgcag cggcgaggtc agcgtgctca tcggcacgcg
     2701 ctccgccgtg ttcgcgccgt tcgcgaagct gggcctgatc gtggtggacg aggagcacga
     2761 cggctccttc aagcagcagg acgggctgag gtactcggct cgtgacgtgg cggtgaaacg
     2821 tgcgcagaca ttggacatac cgctgctgct cgggagcgcc acgccttcac tggaaagcct
     2881 caacaacgcc atggccggcc gctacgccca ccttcggctg cgccaccggg cggggggcgc
     2941 ccggccgccc cggttccacc tgctcgacat ccgcggtcag tccctcgatg acggcctcag
     3001 ccaggatctg ctgcgcatcg tgggccagca tctcaaccgg ggcgggcagg ccctggtctt
     3061 tctgaaccga cgcgggtacg cgcccaccta cctgtgtccc cgctgcagct ggcaggctgc
     3121 ctgcgggcac tgcgagatgc gactgacgct gcacctgtcg ccgcgggcgc tgctctgcca
     3181 ccactgcggc catcagcgac cgctgccgag cgcctgcccg gactgcgggc acgcggagct
     3241 gattgccgtc ggtgccggta ctcagcgcac cgaggccggg ctggctaagc ggttcccagg
     3301 gattcctctg tatcgtgtcg accgcgatac cacccgaagc cagcgtcgca tggaggccca
     3361 gttcgaggcc atccacaagg gcgatccggc aatcctcgtc ggcacgcaga tgctggccaa
     3421 agggcaccac tttccgaagg ttaccgtggt ggccgtggtc aacgccgacg gcgggttcct
     3481 cagcgccgac taccgcgcac ccgagcgcac ggcccagctc atcacccagg tcgccgggcg
     3541 cgcgggtcgt gccgaggatc ccggcgaggt gtggatccag acctatcagc cggacggcgt
     3601 gctgctgcgc tcgctcatcg atgagggcta cgacgggttc gcccggcggg agctggcgat
     3661 tcgcaagtcc agcggcctgc cgcccttccg gcccatggcg ttgttgcggg cggagagccg
     3721 ggacgccgag agcgcccgaa attttttgac cagcgtccgc gaccgcctgg gaagcccctt
     3781 agaagtgctc ggcccggctc cagcgcccat cgcccgagta gccaatcgcc tccggtttca
     3841 gctcatggtg atggcagact cgcgccgcga gctgcatgct ggcatccggc cgatacgaga
     3901 cctcaccgcg ccaggcacca tccgctggtc tgtggacatc gatccctacg atacgttctg
     3961 acctttaccg gccgccccaa cgggcgttca ccggacatcg tggcggacga cccgccgcga
     4021 gcctatcatt cgcagcccat ggccaacgat tttgcaaacc gactcaatcc gccgacgacc
     4081 cggcgcccca aggcccggcc gccctccgcg acacgtcgca aggagaggcg tttcagcggt
     4141 tcgagcttcg cttccggtct gctgatcggc ggcgcgtgca tggccgtggc tttgaacctc
     4201 cctgacccgc agcccgaacg cccgcccgcc gcagctaccg cggcaagcgc accgcccgga
     4261 tccgtgacct tcgagttcga taaggatttg cgcgacgcct ttgtggaggc ggaccctggg
     4321 cagtacgaga cggacgccgg ccacgacgga tcccgaccgg tcagctatct catccaggcg
     4381 gcgtcgtttc cggtagccgc cgatgccgac tcccttcagg cagagcttgc gcaaatgggc
     4441 ctggcggccc agtcggttcc ggcgcggctt aacgccggca cctggttccg ggtcaccgtc
     4501 ggcccgttcg acagccagct gaaggccaat cgagcgctta cgcagcttcg cgaaatcgat
     4561 ctggacgcgt tcatgatcca gcagcgcctg cccgaaccac ggtgaccaaa ccccgttggc
     4621 ggaaccccga tggcggaacc ccgttggcgg aaccccgttg aaatgccggg cagagggccc
     4681 cactatgacc agttcgcaat cggttgaaga gctaaatggc ggatatccac gggacgacca
     4741 tcgtcagcgt gcgcaacgac aggcaggtgg ccattggcgg agacggtcag gtctccatgg
     4801 gccagaccat catgaagggc aatgcccgca aggtgcggcg cctgcatgac gacaaggtcc
     4861 tggctggttt cgccggcgga accgccgacg ccttcaccct tttcgagcgc ttcgagggcg
     4921 tcctcgacaa gtatcacggc aacctcaccc gtgccgccgt ggagctggcc aaggactggc
     4981 ggtcggaccg cgcccttcga cgtctcgagg cccttctggt ggtggcggac cgggaggcca
     5041 cgctcctggt gagcgggacc ggcgacgtca tcgagccgga agccggcgtg atcgccatcg
     5101 gctccggcgg cccctttgcc caggcggcag cgcgggccct gaacgagaac acggaactgg
     5161 atcccgccga catcgtcgag aaatccctgt ccatcgccgc ggacatctgc gtctacacca
     5221 accatcaccg gaccatcgag gtgctggatc tgacatgacc cagatgacgc cgagagagat
     5281 cgttcacgag ctcgataagc acatcatcgg ccaggacgat gccaaacggg ccgtggccat
     5341 cgcgctgaga aaccgctggc ggcggatgca gctcgaagag gagatgcgcg aggagatatc
     5401 gcccaagaac atcctgatga tcggg
//
LOCUS       DBNHLM010000268         7240 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_697046, whole genome shotgun sequence.
ACCESSION   DBNHLM010000268 DBNHLM010000000
VERSION     DBNHLM010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7240)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7240)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7240
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_697046"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="AC16QF_12485"
     CDS             complement(<1..321)
                     /locus_tag="AC16QF_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HIR6449423.1"
                     /translation="MSIKWQEYSAEGLWDELITAGGRARPGAGSLCRMLAKLSDEELN
                     ARKAAAELAIRSMGITFTVYTGEGSGSIDREWPFDIIPRLLLKKEWDQISAGLTQRVK
                     ALNCF"
     gene            472..1656
                     /locus_tag="AC16QF_12490"
     CDS             472..1656
                     /locus_tag="AC16QF_12490"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6449424.1"
                     /translation="MLDGAVHPDFADVAAALIRQIPRGRDGGSAVCVYHRGRCVVDVW
                     GGIRDHHGNPWEHDTTAPSFSTTKGVIATLLHILVDQGKAGYDDPIAHHWPEFGARNK
                     ENITIRQALCHEAGLYRVADMIRRPQEMLDWGHMKRVIADAEPAHEPGSSHGYHALTY
                     GWLIGGVIEAVAGRPLQAVLQDELVDPLGLDGMFIGMPHNELHRRAELIHGRVRPPRA
                     TEPWQETLLEWVDAALGQLGVELAEFSAAMDVFSEDFDWNADETAQAIIPAANGQFTA
                     RSLARMYAMIAQGGELDGVRVLSEGRVREITEVRSRTRDRVLFIPMHWRMGYHRAFAF
                     GVSAPHAFGHYGYGGSGAFCDPSRELAVALILNSGAGTPMGGRYMPLVARAAIRAADR
                     CR"
     gene            1653..2495
                     /locus_tag="AC16QF_12495"
     CDS             1653..2495
                     /locus_tag="AC16QF_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010448255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HIR6449425.1"
                     /translation="MSRQASPAGVDEARLEAFLARHRHLLVITGAGCSTPSGIGAYRD
                     EAGEWLRAEPVQMRDFVASEAARRRYWARSMHGWRAFRDAAPNAAHQALADLERRGVV
                     RGLITQNVDGLHQRAGHDDVIELHGSLRRVVCLECGVSREREEVQRWLEARNGFVLDA
                     SASLAPDGDADLVIDDLSAFEVPRCADCGGLLKPDVVFYGDSVPRDRVDACYAGVAQA
                     DAVLVVGSSLMVFSSFRFVRRAVDLGKPVAAVNRGRTRADAWLEFKVDEDCAAVLERI
                     AANL"
     gene            2717..3535
                     /locus_tag="AC16QF_12500"
     CDS             2717..3535
                     /locus_tag="AC16QF_12500"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6449426.1"
                     /translation="MGGYTTREVSDLIGLKPDQVRHYVRRSLLRPARGDRGEYRFNFQ
                     DVVLLRSAKGLLDADVSSRKAYRVLLKLQSELSQVKSLAAVRIFADGNNVVVREDNQV
                     WDVETGQGQLDFTVRELADNVATLAQRNLIVLRESDGLDSDEWYNLGLDLEEVDPDRA
                     PDAYSRAIELNPKNADAHVNLGRLMQLKGNLKFAKRHYELALVAVSNHQLAYYNLGTV
                     YDELDDMDKAAEYYRRAPGVPDAHFNLSRIYQMQGDEVSALRHMRQYRELLDVE"
     gene            complement(3560..4213)
                     /locus_tag="AC16QF_12505"
     CDS             complement(3560..4213)
                     /locus_tag="AC16QF_12505"
                     /inference="COORDINATES: protein motif:HMM:NF015863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR6449427.1"
                     /translation="MLVLVYLLATGVPLQYAAELDLGQRHVASEWVLDWYGLQAPDEA
                     TRSGPAVMLGGTLFLDGNEQAGLERLVGAIPAGGILAVASSDEVWLFHPGVPDPERTA
                     FRPNIRRIGSLDGQIYLDTTEGLLAADGLLMNWTPTNAPAGAIEWGRVTPLSAGEAAA
                     YRQRFRGRMLTAERWLQDLHSGRFFGTPGVIIVDVASLLMLILAGTGIVLWWRSRRG"
     gene            complement(4254..4778)
                     /locus_tag="AC16QF_12510"
     CDS             complement(4254..4778)
                     /locus_tag="AC16QF_12510"
                     /inference="COORDINATES: protein motif:HMM:NF016121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR6449428.1"
                     /translation="MIIGCVLTLFGPALLAGAAVYQKPDEFLAEAFVGDAPSAQALWI
                     KAELKHELSQVLAHPPGLRVRYWQAGSRTAWILDEIGKDLPITAGIVINDGAIEDVRV
                     LEFRESRGWEIRYPFFTRQFQNARLQGDRLTQNIDGITGATLSVRAMKRMATAALLLH
                     EHSSASSTTLAKAP"
     gene            complement(4850..6103)
                     /locus_tag="AC16QF_12515"
     CDS             complement(4850..6103)
                     /locus_tag="AC16QF_12515"
                     /inference="COORDINATES: protein motif:HMM:NF019042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR6449429.1"
                     /translation="MRVVVVMAAMALVSSATAEEVTLEQLWAVVQAQQAQIEALTDAL
                     ETTQAQLASTEEQVEVAEEQIGLTADYLAEIEASSTRGSRTQLGGYGELHYNNVDARD
                     PDESFKEMDFHRFVLFFSHEFTDRIRFHSELELEHSLVKDTADGSNGGEVELEQAYVE
                     MDLNENHWTRAGLFLLPVGILNETHEPNTFYGVERNDVESIIIPSTWWEGGVAAGGRY
                     GNGLSWEAGVHSGLAMPTDGGNAFRVRSGRQKVSNASAENLAYTLRLKYTGIPGLELA
                     GSYHHQSNPSQMDEDGLDGGDLFSFHGIWSQGPFTLKGLWARWNFDGDLVESSGADRQ
                     TGWYLEPSVRLDLAGTDWGFYGRYEDLEGGRTQDRFDQWEVGFNFWPTENVVLKFDYR
                     DRDHDLETELGRDFKAIDLGVGYQF"
     gene            complement(6220..>7240)
                     /locus_tag="AC16QF_12520"
     CDS             complement(6220..>7240)
                     /locus_tag="AC16QF_12520"
                     /inference="COORDINATES: protein motif:HMM:NF019292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HIR6449430.1"
                     /translation="IMAPYMSCGARLTVYALFAAAFFPTGGQNVVFALYLIGIVVAVL
                     SGLAVRRYLISDSRSTFLLELPTYHLPTMKGLLLQTWQRLKGFVLRAGKAIVAVVIVL
                     NVVGSVGTDGSFGNQNTEKSVLSALGRTITPIFHPMGISQENWPATVGIFTGIFAKEV
                     VVGTLDALYAPGEVASADDLAGQLRAAAASVPANLAELGEQLTDPLGIGLGALDDVAE
                     AASDQEVEINTIQAMRELFHGKLGAFSYLLFILLYMPCVATIGAIFKEIGGFWAAFST
                     SWSVTVAYSAAVICYQLGNLAEQPGPAMLWIAGVAAATVAAFAGLVAMGRRRRQRLIP
                     LVNLE"
BASE COUNT         1228 a         2337 c         2397 g         1278 t
ORIGIN      
        1 gaagcagttc agggccttga cgcgctgagt caaccctgcc gagatctggt cccactcctt
       61 cttcaggagc aggcggggga tgatgtcgaa cggccactca cgatcgatgg aaccgcttcc
      121 ctctccggta tagacggtga aagtgatccc catggatctg atggccagct ccgctgccgc
      181 cttgcgggcg ttgagctctt cgtcgctcag cttggcaagc attcggcaga ggctgccggc
      241 acccggccgg gcgcggccgc cggccgtgat cagctcgtcc cacagccctt cggccgagta
      301 ctcttgccac ttgatgctca tgctcgaatt cgatcgcgcg ccgccaggtt tgcccgcggg
      361 tcattctacg ggacatggcg cttgtgatgg cagccttctc gatgctggtg cgcattgctt
      421 gcgggttcca taaaatgtcc gccccctaat cccaggactc tgatggcggc catgctcgac
      481 ggcgctgttc atcccgactt cgcggacgtt gccgccgccc tcatccggca gatacccaga
      541 gggcgcgatg ggggctcggc ggtgtgtgtc tatcatcgag ggcgctgcgt tgtcgacgtc
      601 tgggggggca tccgcgatca tcacggtaat ccctgggaac acgatacgac tgcgccttcg
      661 ttctccacga ccaagggggt catcgccacg ctgctgcaca tcctggtgga tcagggaaag
      721 gcgggatacg acgaccccat tgcgcaccat tggcccgagt tcggcgcccg caacaaggag
      781 aacatcacca tccgccaggc gctctgccac gaggccgggc tctaccgcgt ggcggacatg
      841 attcggcggc ctcaggagat gctggactgg gggcacatga aaagggtcat tgccgacgcc
      901 gagcccgccc acgagcctgg cagctcccac ggttatcacg cgcttaccta cggctggctc
      961 atcggcggcg tcatcgaggc ggtggccggg cgtcccctgc aggcggttct tcaggacgag
     1021 ctggtcgacc ccctgggcct cgacggcatg ttcatcggca tgccccacaa cgagctccac
     1081 cggcgtgccg agctgatcca cgggcgcgtc cggccgccta gagccaccga gccgtggcag
     1141 gaaaccctgc tggaatgggt cgacgcggcg ttggggcagc tgggggtgga gttggcggaa
     1201 ttcagcgcgg ccatggacgt tttttccgaa gacttcgact ggaacgccga cgagacggcg
     1261 caggccatca tcccggcggc aaacggtcag ttcacggccc gctcgcttgc gcgcatgtat
     1321 gccatgatcg cccagggcgg cgagctggac ggggtcaggg tcctgtccga ggggagggtc
     1381 cgcgagatca ccgaggtccg cagccgtacc cgtgatcgcg tgctcttcat ccccatgcac
     1441 tggcgcatgg gctatcaccg ggcattcgcg ttcggggtca gcgcgcccca cgcgttcgga
     1501 cattacgggt atggcgggag cggagcgttc tgcgatccga gccgggagct tgcggtggcg
     1561 ctcatcctga acagcggagc gggtacccca atgggcggcc gctacatgcc gctggttgcg
     1621 cgcgccgcga ttcgcgccgc cgatcggtgc cggtgagccg gcaagccagc ccggccggcg
     1681 tcgacgaagc ccggctggaa gcattcctcg cccgtcatcg gcatctgctg gtcattaccg
     1741 gcgcgggttg cagcacgccg tcgggaatcg gggcctatcg ggacgaggcg ggagaatggc
     1801 tgcgtgccga gccggttcag atgcgtgact tcgttgccag cgaggcagcg cgtcggcgct
     1861 actgggcccg gagcatgcac ggctggcggg cgtttcgcga tgccgcaccc aacgccgctc
     1921 accaggcgct ggcagacctg gaaaggcgcg gcgtcgtgcg gggactgatc acccagaacg
     1981 tcgacgggct gcaccagcgg gcgggacatg acgacgtcat cgagctgcac ggcagcctgc
     2041 ggcgggtcgt ctgcctcgaa tgcggggtga gccgcgagcg cgaggaggtg cagcgatggc
     2101 tggaagcccg caacgggttc gtcctcgatg ccagtgccag ccttgcgccc gacggcgatg
     2161 cggacctcgt catcgacgat ctctccgcct tcgaagtgcc acgctgtgca gactgcggcg
     2221 gcttgctcaa gccggacgtg gtgttctacg gagactccgt gccccgcgac cgggtggatg
     2281 cgtgctacgc gggcgttgcc caggcagatg ccgttctcgt cgtgggctcg tccctgatgg
     2341 tcttttcgag cttccgtttc gtgcgtcgcg cggtggacct gggaaagccg gtggccgcgg
     2401 tgaaccgggg gcgaacccgg gcggatgcat ggctcgagtt caaggtcgac gaagactgtg
     2461 ctgctgtgct ggaacgaatc gccgccaatc tctaggtggg ccttgtggcg ggcttccggc
     2521 ccggctcgca gcagggctcg cagacgcgcc tccacccggg cctccgcccg gggactttgc
     2581 ccggcccgtg ttgagggctc accaccccta cgctatagtg ccggcgttcc ctgccgccgg
     2641 cggtctcgaa gaggcgggaa gccgtggcta gacacctcgg tcaggcacca atgaaggaac
     2701 cgcatctgcc aatctgatgg gcgggtatac cactcgggaa gtctctgatc tcatcgggct
     2761 taagcccgat caggtccgtc actacgttcg gcgcagcctc ctgcggccgg ctcgtggaga
     2821 ccgcggcgag taccgcttca atttccagga cgtcgtgctt ctccgcagcg ccaagggtct
     2881 gctcgacgcg gacgtttcct cgcgcaaggc ctaccgggta ctgctgaagc tgcagtcgga
     2941 gctcagccag gtcaaatcgc tggcagcggt gcgcatcttc gccgatggca acaacgtggt
     3001 ggtccgggaa gacaaccagg tctgggacgt ggaaacgggg caggggcagc tggacttcac
     3061 cgtgcgggag ctggcggaca acgtggcgac cctggcccag cgcaacctca tcgtcctgcg
     3121 cgaaagcgac ggtctggaca gcgacgagtg gtacaacctg ggcctcgacc tcgaggaggt
     3181 cgatccggac cgcgctccag acgcatactc ccgggccatt gagctcaatc ccaagaacgc
     3241 cgacgcccac gtgaacctcg ggcggctcat gcagctcaag ggcaatctca agttcgccaa
     3301 gcgacactac gagcttgccc tcgtcgcggt ctcgaatcat cagctcgcct actacaacct
     3361 cggaaccgtt tacgacgagc tcgacgacat ggataaggcg gccgagtact accggcgtgc
     3421 gcccggcgtg ccggatgcgc atttcaacct ctcccgcatc taccagatgc agggtgacga
     3481 ggtgtctgcg ctcaggcaca tgcgccagta cagggagttg ctcgacgtcg agtgaccgtc
     3541 ggccggtccg gcacggctct cagccgcgtc tcgaacgcca ccacagcacg atcccggttc
     3601 ctgccaggat cagcatcagc agcgacgcca cgtcaacgat gatcacgccc ggcgtgccga
     3661 agaaccgccc gctgtgcagg tcctgcagcc agcgctccgc ggtcagcatc ctgccccgga
     3721 atcgctgccg gtaggccgct gcctccccag cgctcagggg cgtcacgcgt ccccactcga
     3781 tggcgccggc gggcgcgttc gtcggggtcc agttcatcag cagaccgtct gctgccagca
     3841 gaccctcggt ggtgtcgagg tagatctgac cgtccaggct gccgatgcgc ctaatgttgg
     3901 gccggaatgc cgttctttcc gggtcgggta ccccggggtg gaaaagccag acctcgtctg
     3961 aactcgccac ggccagaatc ccgcccgccg gtatcgcgcc caccaggcgt tcgagcccag
     4021 cctgctcgtt tccgtccagg aacagggttc cgccgagcat cacggccggg ccgctgcgtg
     4081 ttgcctcatc gggcgcctgt agcccgtacc aatcgagcac ccactcgctg gcaacgtgac
     4141 gttgcccgag gtccagctct gcggcgtatt gaagcggcac cccggtagcg agcaggtaga
     4201 cgagcacgag caccgcgcag atgcccgcgg ttctgtgcca ttgattgacc cgtctagggt
     4261 gccttggcga gcgtggtgct gctggcgctg gaatgctcat gaagcagaag cgcggccgtg
     4321 gccatacgct tcatggcccg gacggagagg gtggcgccgg taataccgtc gatgttctgg
     4381 gtcaggcgat cgccctggag ccgggcattc tggaactgcc gcgtgaagaa cggatagcgg
     4441 atttcccagc cccggctttc gcgaaactcg aggacccgta cgtcctcgat ggcgccgtcg
     4501 ttgatcacga tcccggccgt gatgggcagg tccttgccga tctcatccag gatccaggca
     4561 gtccgggatc ccgcctgcca gtagcgtacg cgcagcccgg gcgggtgagc gagcacctga
     4621 gaaagctcgt gcttgagctc tgctttgatc cacaacgcct gggccgacgg ggcatcgccg
     4681 acgaaagcct cggcgagaaa ctcgtcaggt ttctgataca cggccgcgcc ggccagcagg
     4741 gccggcccga aaagggtgag aacacaaccg ataatcattc ttattttcat atgggtcgat
     4801 tttgccgtga tcggtgcaaa aatcaattcc gggcttgtaa gacggctgct cagaactggt
     4861 agccgacgcc caggtcgatg gccttgaagt cgcgcccgag ctccgtctcc aggtcatggt
     4921 cgcgatctcg gtagtcgaat ttcagcacca cgttctccgt gggccagaaa ttgaagccga
     4981 cttcccactg atcgaatctg tcctgggtcc gaccgccttc gagatcttcg tagcggccgt
     5041 agaatcccca gtcggtgccg gccagatcga gccggacgct gggctccagg taccagcccg
     5101 tctgccgatc ggcgcccgag gactcgacga gatcgccgtc gaagttccag cgcgcccaga
     5161 ggcctttcag agtgaaaggc ccctggctcc agatgccgtg aaagctgaac aggtcgccgc
     5221 cgtcgagacc gtcttcgtcc atctggctgg ggttgctctg atggtgatag ctgccggcca
     5281 gctcgagccc gggaatcccg gtgtacttca ggcgcagggt gtaggcgaga ttttcggcgc
     5341 tggcgttgga cactttctgg cggccgctgc gcacgcgaaa cgcattgccg ccgtcggtgg
     5401 gcatggccag gccgctgtgc acgccggcct cccaggacag gccgttgccg tagcgccccc
     5461 cggccgccac gcccccctcc caccaggtgg acgggatgat gatgctctcc acgtcgttgc
     5521 gctcgacgcc gtagaaggtg ttcggctcgt gggtctcgtt gagaatgccg acgggcagca
     5581 gaaaaagacc agccctggtc cagtggttct cgttgaggtc catttccacg taggcctgct
     5641 ccagctccac ctcgccgccg ttgctgccgt ctgcggtgtc cttgaccagt gaatgctcca
     5701 gctccagctc cgagtggaac cggatccggt cggtgaactc gtgactgaag aacagcacga
     5761 agcgatggaa gtccatctcc ttgaaactct cgtccggatc gcgggcgtcg acgttgttgt
     5821 agtgaagctc gccgtaaccg cccagctggg tgcggctgcc gcgcgtgctg gacgcctcga
     5881 tctccgcgag gtagtcggcg gtgaggccga tctgctcctc ggcgacctcc acctgctcct
     5941 cggtgctggc gagctgggcc tgggtggttt cgagcgcgtc ggtcagcgct tcgatctggg
     6001 cctgttgagc ctgcaccacg gcccacagct gctccagggt gacttcctcg gcggtggcgg
     6061 acgaaaccag ggccatggcg gccataacca cgacgacacg catgagcgat tctcccgaac
     6121 gtgttggatc aaggggaacg ggagtctagc ggatgtgttt atgaatagga attattatta
     6181 tttagattaa ggtattggtc ttgccgcagc gccgtgggcc tattccaggt tgaccagcgg
     6241 aatcaggcgt tgccgacgtc tgcggcccat ggcgaccagc cccgcgaagg cggcgacggt
     6301 ggccgccgcg acgcctgcga tccacagcat ggccgggccc ggctgctcag cgaggttgcc
     6361 cagctggtag cagatgaccg cggccgagta agcgacggtc acagaccagc tcgtgctgaa
     6421 cgccgcccag aagccgccga tctccttgaa gatggcgccg atggtggcca cgcagggcat
     6481 gtacagcagg atgaacagca ggtagctgaa cgcgccgagc ttgccgtgaa agagttcgcg
     6541 catggcctga atggtgttga tctcgacctc ctggtcggag gcggcctcag ccacgtcgtc
     6601 cagggcccct agcccgatgc ccagcgggtc ggtgagctgc tcgccgagct cggcaagatt
     6661 ggcggggacc gaggcggcgg ctgcccggag ctggccggcg aggtcgtccg cggacgccac
     6721 ctcgcccggt gcgtacaggg catccagagt cccgaccacg acctccttgg cgaagatgcc
     6781 ggtgaagatg ccaacggtag ccggccagtt ttcctggctg atgcccatgg gatgaaagat
     6841 cggggtgatg gtacgcccca gagcggagag cacagacttc tcggtgttct gattcccgaa
     6901 cgagccgtcg gtgcctaccg atcccaccac gttgagaacg atcaccacgg ccacgatggc
     6961 cttgccggcg cgcaggacga agcctttgag ccgttgccag gtctgcagca gcagcccctt
     7021 catcgtcggc aggtggtacg ttggcagctc cagcaggaac gtgcttcggc tgtcggagat
     7081 caggtaccgt cgaaccgcca atcccgacag caccgcgacg acgatgccga tcaggtagag
     7141 ggcgaatacc acgttctgcc cgccggtggg gaagaacgcc gcggcgaaaa gcgcgtagac
     7201 cgtcagccgg gccccgcagg acatgtaggg agccatgatc
//
LOCUS       DBNHLM010000269         3285 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_577997, whole genome shotgun sequence.
ACCESSION   DBNHLM010000269 DBNHLM010000000
VERSION     DBNHLM010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_577997"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1349)
                     /locus_tag="AC16QF_12525"
     CDS             complement(120..1349)
                     /locus_tag="AC16QF_12525"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HIR6449431.1"
                     /translation="MPEAAPPGTATADSNPWPISQQLPPDTMGTSGPARALRFGSATL
                     TVTTMYTLLITFFTLAIVFSFLCSLWEAVLLSITPAYAEIQKQHGSAIGRHLSTFKSN
                     IDRPLAAILTLNTIAHTVGAIGVGGQAAAIWAETSPWVTKAIIPVAMTLAILILSEII
                     PKTIGATFWKELAPFTVRSLLVVIAALGPLVWLSQRVTRILKREAGDSVLSRSDFLAM
                     TKIGERAGVFEKSESEIIKNLLRFEQVCAKDIMTPRTVVIAEPESRTVAEFYAAHDQL
                     RFSRIPIFSDAKDHITGYVLKDELLSSLVQQEGDRTLGDFRRDIMVAKEDQPLPDLFS
                     RLVEKREHIALVVDEFGGMAGIVTMEDVIETLLGLEIVDESDQVYDMQAHARAQWARR
                     AKAHGLTETDAVGPTSS"
     gene            1403..2278
                     /locus_tag="AC16QF_12530"
     CDS             1403..2278
                     /locus_tag="AC16QF_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007943000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR6449432.1"
                     /translation="MGAIEQPFEMIRYERPGEHVARIMLAREETRNAQNVQMLYEIDA
                     ALDAAMQDDDVRAVIIGADGPHFSSGHDLKGGGRIGDFGPPKMGVGGFGKPGQEGHMS
                     HEQEVFIGFCWRWRNMPKPTIAAVQGKAIAGGLMLVWPFDLIVASEDAQFSDPVVTFG
                     VNGHEYFVHAWEVGHRKAKEMLFTGESISAQEGKELGMVNRVVKREDLMSETLALAEK
                     IARQPSMGLKLSKLAVNQSLDAQGMWTAIQSAFNLHHLGHAHMRIMHGQAVEPAGAKK
                     IKTQARADKPKLTGP"
     gene            complement(2293..>3285)
                     /locus_tag="AC16QF_12535"
     CDS             complement(2293..>3285)
                     /locus_tag="AC16QF_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HIR6449433.1"
                     /translation="RPERNLAEDQPTNVLRLARAIRPSGSTGTAQQVFYDWGIGSYYD
                     PFVGGVTGRGINKNIMDGYRYLVQNYAPGDQIFLFGFSRGAYTVRSLCGLINNCGILK
                     RPDARLIQAAFDHYKRPGSAHHPDGPRSREFRAEHAHPSRRIRFVGVWDTVGALGIPF
                     SFLGMLDREDEFYDTKIGPNIEIARHAMAIDERRSDFEPTIWQQRAGTDISQVWFAGA
                     HGNVGGGLYREGKGGRLSDIALAWMINEARGAGLSVEGHLGDRLRPDPLAPVYGSRRH
                     IYRSRPRADRPIDHGSAPVLVHESVRQRWDALPGYRPRNLVRYLEINGWPTLVP"
BASE COUNT          576 a         1013 c         1120 g          576 t
ORIGIN      
        1 atgcggtgaa cgccattccc tacgtttgtc agtccgagcc gggaatcagg acgtttctgg
       61 atctgccctg gatcatgtac cgtggtgctg gcgccccatg agcctgccgc caggcgtcgt
      121 caggaagacg tcggcccgac agcgtccgtt tccgtgagtc catgggcctt tgcgcgtcgg
      181 gcccactgcg cccttgcgtg ggcctgcata tcgtagacct gatcggactc gtcgacgatc
      241 tccaggccga gcagcgtctc gatcacgtct tccatggtga cgatgccggc catgccgccg
      301 aactcgtcca ccaccagggc aatgtgctcg cgcttctcca cgagcctcga gaacaggtcc
      361 ggcaggggct ggtcctcctt cgccaccatg atgtcgcggc ggaagtcgcc cagggtcctg
      421 tcaccttcct gctgaaccag gctggacagc agctcgtcct tgagcacgta gccggtgatg
      481 tggtctttcg catcgctgaa tatcgggatg cgggaaaagc gcagctgatc gtgcgcggcg
      541 tagaactcgg ccacggtgcg gctttccggc tcggcaatca ccacggtgcg cggcgtcatg
      601 atgtctttgg cgcaaacctg ctcgaagcga aggagattct tgatgatctc cgactccgac
      661 ttctcgaaga cgccggcccg ctcgccgatc ttggtcatgg cgagaaagtc ggagcgcgag
      721 aggacgctat ctccggcctc ccgcttgagg attcgggtga cccgctggct cagccagacg
      781 agcggtccca gcgcggcgat caccacgagc agcgagcgca ccgtgaaagg cgccagctcc
      841 ttccagaagg tggcgccgat ggtcttcggg atgatctccg acaggatgag aatggcgagc
      901 gtcatcgcca cgggaatgat ggccttggtg acccaggggc tggtttccgc ccagatggcg
      961 gcggcctgac cacccacgcc gatggcgccg acggtatggg cgatggtgtt gagcgtgagg
     1021 atggcggcca gcggccggtc gatgttgctc ttgaaagtgc tcaggtggcg cccgattgcc
     1081 gagccgtgct gtttctgaat ctcagcgtac gcgggcgtaa tgctcagcag cacggcctcc
     1141 cacagggagc agagaaaaga gaaaacgatg gccagcgtga agaaggtgat cagcagcgtg
     1201 tacatggtcg tgactgtgag cgttgcggac ccaaagcgca acgccctggc cggaccggac
     1261 gttcccatgg tgtcgggcgg aagttgctgg ctgatcggcc agggattaga gtcggcagtc
     1321 gccgtgcccg ggggcgcggc ttccggcaaa cagggtccga gccgcggacg cgcgcgggcc
     1381 cgccttgaat tggggggatg tcatgggtgc cattgagcag cctttcgaga tgatccgcta
     1441 cgagcggccc ggcgagcacg tggcgcgcat catgctggcg cgggaagaga cccgcaacgc
     1501 gcagaacgtc cagatgctct acgagatcga cgcggcgctg gatgcggcca tgcaggacga
     1561 cgacgtgcgg gcggtgatca tcggcgccga cggcccgcat ttctcgtcgg gccacgacct
     1621 gaagggcggg ggccgcatcg gcgacttcgg acccccgaaa atgggcgttg gcggtttcgg
     1681 caagcccggg caggaaggcc acatgtctca cgagcaggag gtcttcatcg gcttctgctg
     1741 gcgctggcgc aacatgccca agccgaccat cgccgccgtg caggggaagg ccatcgccgg
     1801 agggctcatg ctggtatggc ctttcgatct catcgtcgcc agcgaggatg cgcagttctc
     1861 cgaccccgtg gtcacctttg gcgtcaacgg ccatgagtat ttcgtgcacg cctgggaagt
     1921 cgggcaccgc aaggctaagg agatgctgtt taccggggag tcgatttccg cccaggaggg
     1981 taaggaactc ggcatggtca accgcgtggt caagcgggaa gacctgatga gcgagaccct
     2041 ggcccttgca gaaaaaatcg ctcggcagcc cagcatgggg ctgaagctgt ccaagctcgc
     2101 cgtcaaccag tccctggatg ctcagggcat gtggacggcc attcaatcgg cgttcaacct
     2161 gcatcacctc ggccacgccc acatgcgcat catgcacggc caggccgtgg agcccgccgg
     2221 cgctaagaag atcaagactc aggcccgggc ggacaagccc aagctcactg gcccttgaag
     2281 cgagccgccg gctcagggaa ccagcgtcgg ccagccgttg atctcaaggt agcggaccag
     2341 gttgcgcggc cggtagcctg gcagcgcatc ccagcgctgg cgaacgcttt cgtgaacgag
     2401 caccggagcg ctgccgtggt cgatgggacg atccgctcgg ggccgggagc ggtagatgtg
     2461 gcggcgtgac ccgtacacgg gcgcgagggg atcgggccgc agccggtcac ccaggtggcc
     2521 ctccactgaa agcccggcgc cccgggcttc gttgatcatc catgccagcg cgatgtccga
     2581 aagccggcca cccttgcctt cccggtagag cccgcccccg acgtttccgt gggcgcctgc
     2641 aaaccagact tgtgagatgt cggtgcccgc gcgctgctgc cagatcgtag gttcgaagtc
     2701 ggagcggcgc tcgtcgatgg ccatggcgtg gcgggcgatc tcgatgttgg gaccgatctt
     2761 ggtgtcgtag aactcatcct cacgatcgag catccccagg aaggagaacg gaatgcccag
     2821 cgcaccgacg gtgtcccaga cgcctacgaa gcggatcctg cggctggggt gcgcatgctc
     2881 cgctcgaaac tcccgggagc gcgggccgtc gggatgatgg gcgctgcctg gtcgtttgta
     2941 atgatcgaat gccgcctgga tgagcctggc gtcagggcgc ttgaggatgc cgcagttgtt
     3001 gatcagcccg cacaggctgc ggaccgtgta ggcgccgcgg ctgaagccga acaggaagat
     3061 ctggtcgccg ggggcgtagt tctgcaccag gtagcggtat ccgtccatga tgttcttgtt
     3121 gattccccgt cccgtcacgc cgccgacgaa gggatcgtag taagagccga tcccccagtc
     3181 gtaaaacacc tgctgggcgg tgcccgtgct gcccgatggc cggatggccc tggccagccg
     3241 caggacgttg gtgggctgat cttcggccag gttgcgttcc ggtcg
//
LOCUS       DBNHLM010000270         8435 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_638128, whole genome shotgun sequence.
ACCESSION   DBNHLM010000270 DBNHLM010000000
VERSION     DBNHLM010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8435)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_638128"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="AC16QF_12540"
     CDS             <1..461
                     /locus_tag="AC16QF_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449434.1"
                     /translation="FALMAALGFFAMNACADTEEVSSTAYWTIVAPAEEYKMTNGATM
                     TGGGIAHANVINNEGEHLSQWCRGITAADAEGKGLGGAGFCTIIDQAGDAMWVWFSNN
                     ADGQGGTWGVIGGTGAYAGASGGGTSGAPTSMSPDGRSWINTSTGTITTP"
     gene            complement(562..1812)
                     /locus_tag="AC16QF_12545"
     CDS             complement(562..1812)
                     /locus_tag="AC16QF_12545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6449435.1"
                     /translation="MSDYDVVIVGGGLGGSALGGFLADAGRKVLVLERLTEFSDRVRG
                     EWMACWGVVELKKLGLYERFMAAGGHHLSRSIPYDELLPAERAEAIATKFADLHPDVP
                     GPLCMEHVVMQNEAIAWAAESGADVLRGVSGVAVTAGAKPRVRYTHDGVDHDVGCRWI
                     VGADGRGSSVRRQLDIPINEAPLDHLIAGLLIEGADDWPEDLQCLGKFGDLHYLVFPQ
                     GGGKVRIYADYAYQGSARFSGPDGASEMLAAFDLPRVPHSKAVANARPIGPCRSYPSQ
                     DAWVEEPCVEGAVLIGDAAGYNDPILGQGLSVTLRDAHSVGAILAGGDAWSADLFRPY
                     VEERGERLRRLRFAASFVTTLYARFEPGDVERRAEAFRRMGADPSLAVMSLAAILGPE
                     TLPAECFTPAFYERAFGTTENMVA"
     gene            complement(1823..2773)
                     /locus_tag="AC16QF_12550"
     CDS             complement(1823..2773)
                     /locus_tag="AC16QF_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6449436.1"
                     /translation="MAFGHDTTTEEVLQGIDLRGKTALVTGASGGLGAETARALASRG
                     AAVTLAVRNVAKGEDVAESIRESTGSTVDVVELELDSLPSVRACAESWLGNHGPLNLL
                     INNAGVMACPLARTEAGWELQFATNHLGHFLLTLLLLPALRAGAPARIVNLSSGAHRF
                     GGVDFDDIHFERREYDKFVAYGQSKTANILFAVELGRRLEGSGITVNAVHPGVIVTEL
                     GRHLTPEDLANFPGAKSVDELRYKTVESGAATSVWAATSPELEGRSGLYCEDCHVAGP
                     AVEDGVQEGYSSWARDPEAAARLFALSEELVGEKYAGAAA"
     gene            2908..4398
                     /locus_tag="AC16QF_12555"
     CDS             2908..4398
                     /locus_tag="AC16QF_12555"
                     /inference="COORDINATES: protein motif:HMM:NF014165.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449437.1"
                     /translation="MSAYSELEAKAREVARLREVAAITHWDEACMMPRGGGAGRSEAL
                     ASLATLIHERVADPEVGELLNRARGEGFELDDWQSANVTNMEREFVEATAIPSDLVRA
                     SRLAQMKCEQAWREARARNDWETVKPLLAEVIGLARQQADALADAMHLSRYDALLEVY
                     EPELRIADIEPVFEDLKAFLPGALETALDRQGEVAPLEGPFTVEEQRQLSLEVMGLLG
                     FDFDRGRLDVSHHPFCGGVPDDTRITTRYSESSFIEALMATCHETGHALYQQGLPRDW
                     RQQPVGDSLGAAVHESQSLLMELQVCRSREFVGFLAPMIRRHFDRSETEPAWSTDNLY
                     GHVTRVARGYIRVDADEITYPLHVILRFELERQLLDGTLAVADLPEAWNDAMQRYLGL
                     STEGNNADGCMQDVHWYAGLFGYFPTYTLGALTAAQWFATARKVMPDLASDIAAGNLE
                     PLVAWLRENIHGRGRQTTMQPLLEEVTGSTLDAGFYKRHIQARYLG"
     gene            complement(4444..5523)
                     /locus_tag="AC16QF_12560"
     CDS             complement(4444..5523)
                     /locus_tag="AC16QF_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015648740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HIR6449438.1"
                     /translation="MHGLQLLVNRNDLEDTRWQQAPVPAPDELAPGEVLLRIDEFALT
                     ANNITYAVAGEMMRYWNFFPAPDGWGRVPVWGFATVVASNHAEVATGERLYGYLPMGS
                     HLLIEAGRVRANQLTDVSAHRVDLPPVYNQFQRVDAASEPAADAAQMLYRPLFMTSFV
                     LDHFLHANGFFGAQSVILTSASSKTALGLALLLARRGDVRVIGLTGRANVGFVEGLGC
                     YDEVVAYEDLATLDAAREVVSVDMAGNGEVLAALHNHFADNLKYSCLVGATHWDHRAG
                     AREMAGPEPELFFAPSHIQDTDWGPGGLEARFADAWDMLVERMSGLVTVVRADDPAEV
                     EAVYQRLLAGEVGPDVGYVLGVDVG"
     gene            complement(5539..6408)
                     /locus_tag="AC16QF_12565"
     CDS             complement(5539..6408)
                     /locus_tag="AC16QF_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6449439.1"
                     /translation="MELDGFETREIELDGVRMHVRIGGAGPPLLLLHGYPQTHLMWHR
                     IAPRLARNFFVVAPDLRGYGASGKPPAGEEYRAYSKRVMAAEMVALMAGLGHERFAVA
                     GHDRGGRVAYRMALDHPEVVSRLVILDIIPTLDQFEALGWRGGLFGYHWYFLAQPPPL
                     PETLIAHEPELYLRTAMSSWAGDFSALDAAFPAYLAAFTPETIRASCDDYRAGAYVDT
                     EIDRADREAGRRIGCPVLALWGEINGPRPGVLEAWQRWADDVQGVGLPCGHFLPEEAP
                     EAVLAAMLEFLKA"
     gene            6708..>8435
                     /locus_tag="AC16QF_12570"
     CDS             6708..>8435
                     /locus_tag="AC16QF_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6449440.1"
                     /translation="MPKQMHITFILLAISSTFAENVRAERTTTTQIEEIVVTAQKREE
                     SMQDVAISMSAMDANAIEKSFSRTIDEITGMSPNLVINPILGNGTVGVSIRGMQHAEV
                     EKSFDPAVAIYQDGIYLSSTTGALLNVWDAERVEVLRGPQGTLFGRNTIGGLVHVIRS
                     KPTGEIGGKIVATIAEDSQEDFKALLNLPEIGGVSLKLSAASSKGGAYIKNASRGTTD
                     GENDLFMFTIDALWQPTDNFDLRVIYDNIDDDTPTRPVTALTPSGEAFGAFCGDNCAV
                     GKESKALYTTYTTQEQFAELHTDAWTIHANWQFHENHKLAFVFGDRDTDELARQEFDG
                     VAADLFWTDRPTFESQTSYELRLESDWNERLRSTLGVFLWESDYTLQQNTGLFNIFNP
                     TSVTNFTASPFYQQDVDSMGVFGQVDWDITDRLTLSLGGRWLDEEKSACMTVTGYSEN
                     PFQQILDLPPGEVVYVDVAGYEKTLDPYPLFPGPGDLTAGSWGEDCPEWAAPVYDPTF
                     DGKESWSEFTPRVGAQYNFDAGMAYVTYSEGFRSGGFNGRATAPDNTGPYDPESVESW
                     EIGGKLMFLD"
BASE COUNT         1581 a         2869 c         2647 g         1338 t
ORIGIN      
        1 ccttcgcgtt gatggccgcg ctgggctttt tcgccatgaa tgcctgcgcg gataccgagg
       61 aagttagcag cacggcctac tggacgatcg tggcgcctgc cgaggagtac aagatgacca
      121 acggcgcaac gatgaccgga ggcggcatag cccacgccaa cgtcatcaac aacgaaggcg
      181 agcacctaag ccaatggtgc cgcggcatca cggcggcaga tgctgaaggc aagggcctgg
      241 gcggcgccgg gttctgcaca attatcgatc aggccggcga tgcgatgtgg gtctggttca
      301 gcaacaatgc tgacggtcag ggcggcacct ggggcgtcat cgggggtacc ggcgcgtacg
      361 ccggcgcgag cggcggcggg acgtccggcg caccaacctc catgagcccg gacggccggt
      421 cctggatcaa cacgtccacc ggcaccatca ccacgccgta ggttcggcac gcccggcttt
      481 cccatggaaa gccgggcgca cggtcccttc caggcccgat catccaaacc ccgcaccgga
      541 ccgcccgggc ggcgccggct gtcaggccac catgttctcc gtggttccga aggcccgctc
      601 gtagaaggcg ggcgtgaagc actcggcggg cagagtctcg ggacccagga tagccgccag
      661 cgacatgacg gcgaggctgg ggtccgcacc catgcgccgg aacgcctccg cccgacgttc
      721 gacgtccccg ggttcgaacc gtgcatacag cgtcgtgacg aaagaggcgg caaagcgcag
      781 gcgccgcagc cgttccccgc gttcttccac gtagggccgg aacaggtcgg cgctccaggc
      841 gtcgccgccg gcgaggatcg cgcccacgct gtgggcatcg cgaagggtca ccgaaaggcc
      901 ctgtcccagg atgggatcat tgtagccggc cgcatcacca atcagcaccg caccctccac
      961 acaaggctct tccacccagg cgtcctggct gggataagac cggcagggac cgatgggccg
     1021 ggcgttggcc actgccttgc tgtggggcac gcggggcaga tcgaaggcgg cgagcatctc
     1081 gctggcgccg tcgggacccg agaatcgagc gctgccctgg taggcgtaat ccgcgtaaat
     1141 cctcaccttg ccgccgcctt gtggaaatac caggtagtgc aggtctccga acttgccgag
     1201 gcactgcaga tcttccggcc agtcgtcggc gccctcgatc aggagcccgg ctatcaggtg
     1261 atccagaggc gcttcgttga tgggaatgtc gagctggcgc cgaacgctgg acccgcggcc
     1321 atcggcgccg acgatccagc gacagccgac gtcatggtcg acgccgtcgt gcgtgtaacg
     1381 cacgcgcggc ttggcgcccg ccgtcacggc aacgccgctc accccgcgca gaacgtccgc
     1441 accggactcc gccgcccagg cgatggcctc gttctgcatc accacgtgct ccatgcacag
     1501 cggtcccggc acatcggggt ggaggtcggc aaatttggtg gcgatggctt ccgcccgttc
     1561 cgcgggaagc agctcgtcgt agggaatgct gcgggataag tgatgaccgc ccgcggccat
     1621 gaagcgctcg tagagtccga gcttcttcag ctccaccacc ccccagcagg ccatccactc
     1681 tccgcgcacc cggtccgaaa actccgtcag ccgctcgagc accagcactt tgcgcccggc
     1741 gtcagccagg aagcccccga gcgcgctgcc gcccagcccg ccgcccacga tcaccacgtc
     1801 gtagtcactc atcggcagac cctcaagctg ccgcgccggc gtacttctcg ccgacgagct
     1861 cctcggacag cgcaaacagc cgtgccgccg cctccgggtc cctggcccag gacgagtaac
     1921 cctcctgaac gccgtcctcc accgcgggac ccgcgacgtg acagtcctcg cagtaaaggc
     1981 cgctgcgccc ctccagctcc ggcgaggtag ctgcccacac gctggtggcg gcaccggact
     2041 cgacggtctt gtacctcaac tcgtccacgg atttcgcacc cggaaagttg gccaggtctt
     2101 ccggcgtgag atgccggccg agctcggtca cgatgacgcc cgggtgcacg gcgttgacgg
     2161 tgatcccgct gccttccagg cgccggccga gctccaccgc aaagagtatg ttggccgtct
     2221 tggactgacc gtacgcgacg aacttgtcgt actcgcgccg ctcgaagtgg atgtcgtcga
     2281 aatccacacc gccgaagcgg tgagcgccgg agctcaagtt gacgatccgc gccggtgcgc
     2341 cggcccgaag cgccggcagc aggagcagcg tcaacaggaa gtggccgaga tggttggtcg
     2401 cgaactgaag ctcccacccc gcctccgttc gggccagcgg gcaggccatg acgccggcgt
     2461 tgttgatgag caggttgagc gggccgtgat tcccaagcca gctttcggca caggcgcgca
     2521 cgctgggaag cgaatccagc tccagctcaa ccacatccac cgtgctgccc gtcgactccc
     2581 ggatgctctc cgccacgtct tcgcccttcg ccacgttgcg caccgcgagg gtgaccgccg
     2641 cgcccctgga tgccagcgcc cgggccgtct cggcgcccag ccccccggag gccccggtga
     2701 ccagcgccgt cttgcccctg aggtcgatgc cctgcagaac ctcctcagtg gtggtgtcgt
     2761 gtccaaaagc catgccgctg atcccccaaa aaatacccgc tcaatcctat ccatccggcc
     2821 atcggggcgc cacggcccaa cgctcccaaa acgacgccga aggtctttat actgcgccgc
     2881 tccaaattca atcccgccag gaagaccatg tccgcctact cggagctcga agccaaagcc
     2941 cgcgaagttg cccgcctgcg cgaggtggca gccattaccc actgggacga ggcctgcatg
     3001 atgcccaggg gcggcggcgc gggccgctcc gaagcgctgg cgtccctggc cacactgatc
     3061 catgagcgcg tggcggatcc ggaagtcggc gagctgttga atcgcgcgcg tggggagggg
     3121 ttcgagctcg atgactggca atcggccaac gtgaccaaca tggagcggga gttcgtggaa
     3181 gccacggcaa tcccttcgga cctggtgcgg gcgagccggc tggcgcagat gaaatgcgag
     3241 caggcctggc gcgaggcccg ggcgcgaaac gactgggaga cggtgaagcc cctcctcgcg
     3301 gaggtgatcg gtctcgcccg tcagcaggcg gatgccctgg ccgatgccat gcatctgtcc
     3361 cgctacgatg cgcttttaga ggtctacgag ccggagctgc gcatcgccga catcgagccc
     3421 gtcttcgagg atctcaaggc gtttctcccc ggcgcgctgg agacggccct ggatcgccag
     3481 ggcgaggtgg cgcccctcga ggggcctttc acggtggagg agcagcgtca gctgtccttg
     3541 gaggtcatgg gcctgctcgg cttcgacttc gatcggggca ggctagacgt cagccatcac
     3601 ccgttctgcg gcggcgtgcc ggacgatacc cgcatcacca cccgttactc tgagtcgagc
     3661 ttcatcgagg cgctcatggc gacctgccac gaaaccgggc acgcgctcta ccagcagggt
     3721 ctgcccaggg actggcggca gcagccggtc ggcgactcac tgggtgcggc cgtccacgaa
     3781 agccagtcgc tgctcatgga gctgcaggtc tgccgcagcc gcgagttcgt cggctttctg
     3841 gcgcccatga tccgccggca cttcgaccgc agcgagaccg agccggcgtg gtcgacggac
     3901 aacctgtacg gccacgtcac ccgcgtggcg cgcggctaca tccgcgtgga cgctgacgag
     3961 atcacctacc cgctccacgt catcctgcgt ttcgagctgg agcgccagct gctggacggc
     4021 accctcgccg tggccgacct gcccgaggca tggaacgacg ccatgcagcg ctatctgggg
     4081 ctgtccaccg agggcaacaa cgcagacggt tgcatgcagg acgtgcactg gtacgcgggc
     4141 cttttcggtt acttcccgac ctataccctg ggcgcgctca ccgccgcgca atggttcgcc
     4201 accgcccgaa aggtcatgcc ggatctcgcc tcggacatcg ccgccggaaa cctcgagcca
     4261 cttgtggcct ggctgcggga gaacatccac ggccgcggtc ggcagacgac catgcagccg
     4321 ctcctggagg aagtcaccgg ctcgacgctg gacgccgggt tctacaaacg gcacattcag
     4381 gcgcgttatc tcggctgagg ccgcctcgcc acgatcggat cgccccaaaa ggggcggcgc
     4441 gattcaaccc acgtcgactc ccagcacgta gcccacgtct ggtcccacct ctccagcaag
     4501 cagccgctgg tagacggcct cgacctctgc cgggtcatcg gcccgaacca cggtgacgag
     4561 ccccgacata cgctccacaa gcatgtccca ggcatcggcg aagcgggctt cgagcccgcc
     4621 ggggccccag tccgtgtcct gaatgtggct cggcgcgaag aacagctccg gttcggggcc
     4681 cgccatctcc ctcgcgccag cgcggtgatc ccagtgggtg gcccccacca ggcagctgta
     4741 cttcaagttg tccgcgaagt ggttgtgcag ggcggccagc acctcaccgt taccggccat
     4801 gtcgacgctc accacttccc gtgcagcatc cagagtggcc aagtcttcat aggccactac
     4861 ctcgtcgtag cagccgagcc cttccacgaa cccgacgttg gcccggccgg tgagaccgat
     4921 cacgcgaacg tccccgcgcc gcgcaaggag cagcgcaagc ccgagagccg tcttgctgga
     4981 cgcgctggtc agaatcaccg actgcgcgcc gaagaagccg tttgcatgga gaaaatggtc
     5041 cagcacgaaa gaggtcatga acaacggccg atagagcatc tgggccgcgt cagcggccgg
     5101 ctcggaagca gcatcgacgc gctggaactg gttgtagacc gggggcaggt cgacgcgatg
     5161 ggcgctgacg tcggtaagct ggttggcccg cacccggccg gcttcgatga gcagatggct
     5221 gcccatgggc aggtagccgt agaggcgctc gcccgtggcc acctcagcgt gattcgacgc
     5281 gaccacggtt gcaaatcccc agaccggcac gcggccccag ccgtcgggcg ctggaaagaa
     5341 gttccagtag cgcatcatct ccccagccac cgcataggta atgttgttgg cggtgagggc
     5401 aaactcgtcg atgcgcagca gcacctcgcc cggggccagc tcgtcgggag ccgggaccgg
     5461 cgcctgctgc cagcgggtgt cctcgagatc gttccgatta accagcagct gaagaccgtg
     5521 cattcatcct ctccctattc aggctttgag aaattctagc atcgcggcca gaaccgcctc
     5581 gggtgcctcc tcgggcagga agtggccgca gggcagcccg acgccttgga cgtcgtccgc
     5641 ccagcgctgc caggcctcga gcactccagg ccgcggcccg ttgatctcgc cccacagtgc
     5701 cagcaccggg cagccgatcc gacggccagc ctcacgatca gcccgatcga tttcggtgtc
     5761 gacgtaggct ccggcccggt aatcgtcgca gctggcgcgg atcgtctccg gggtgaaggc
     5821 cgcgaggtac gcaggaaagg ccgcatcgag ggcagagaag tcgccagccc agctgctcat
     5881 ggccgtgcgc aggtagagct ccggctcgtg ggcgatgagg gtctccggca ggggcggtgg
     5941 ctgagcgagg aagtaccagt ggtagccgaa aagaccgccg cgccacccga gggcctcgaa
     6001 ctgatccagg gtcggaatga tgtccaggat gacaagccgg ctcaccactt ccggatgatc
     6061 cagcgccatg cggtaggcca cgcgaccgcc gcggtcgtgc ccggccacgg cgaagcgctc
     6121 gtgaccaagg cccgccatca gcgccaccat ctccgccgcc atcacccgct tggaataggc
     6181 gcggtattct tccccggccg gtggcttgcc gctggcaccg tagccgcgca ggtcgggcgc
     6241 caccacgaaa aaattccgcg cgagtcgggg ggcaatccgg tgccacatga gatgagtctg
     6301 agggtagccg tgcagcagca ggaggggcgg tccagcgccc ccgatccgaa cgtgcatgcg
     6361 cacgccatcc agttcgatct cgcgggtctc gaagccgtcc agctccattg ttatcccccg
     6421 gcgttctgcc tcgacggccc cgacctactc ccgggcgagc cgggtccaag tgccccgatc
     6481 gttatccaat acccggccct tcaggtcgtt aacttcgtac ccgtaacgtg cccgctcggt
     6541 gcagggaggg gacatttcag accggaatca catcaacccg caccaacacg ctccaggcgt
     6601 ccgagctctc gaggcgactg gactccgttt gtacatcaaa gtagagtccc cgcccgaatc
     6661 gcttccacag cagaagcggc cacgagataa gactatcgag gggaaacatg ccaaagcaaa
     6721 tgcacataac gttcatcctc ctggcgatct cgtcaacgtt cgccgaaaac gttcgtgccg
     6781 agagaacgac caccacgcag atcgaagaga tcgtcgtgac tgcgcagaag cgcgaggaaa
     6841 gcatgcagga cgttgccatc tcaatgtccg ccatggatgc caatgcgatc gagaaatcat
     6901 tctcccgaac gatcgacgag attaccggga tgtcgcccaa cctggtgatc aacccaatcc
     6961 tggggaacgg aacggttggc gtgtcgattc gcgggatgca gcacgctgag gttgaaaaga
     7021 gtttcgatcc ggccgtcgcc atctatcagg acggtatcta tctgtcatcg accaccggcg
     7081 ccttgctgaa cgtctgggac gccgagagag tcgaagtgct cagggggccc cagggcaccc
     7141 tgttcggcag aaacaccatc ggcggcctgg tccatgtcat ccgctccaaa ccgacgggag
     7201 agatcggcgg gaagatcgtg gccaccattg ccgaggacag ccaggaagac ttcaaagcgc
     7261 tgctcaatct gccggaaatc ggtggcgtgt cactgaagct gtcggccgcc agcagcaagg
     7321 gcggcgccta catcaagaac gcgtcccgcg gcaccaccga tggggagaac gacctcttca
     7381 tgttcaccat cgacgcgctt tggcagccca ccgacaattt cgatctgcga gtcatctacg
     7441 acaacattga cgacgacacg ccaacgcgtc cggtcaccgc attgacccca tcgggcgaag
     7501 ccttcggagc cttctgcggc gacaactgtg ccgtgggcaa ggaatccaag gctctttata
     7561 ccacctacac gacgcaggag cagttcgccg agctgcacac cgacgcgtgg accattcacg
     7621 caaactggca gttccacgag aatcacaagc ttgccttcgt cttcggcgat cgcgacacgg
     7681 atgaacttgc gcgccaggag ttcgacggcg ttgcggcaga cctattctgg acggaccgcc
     7741 cgacgttcga aagccagacc agctacgagc tgcgactcga atcggactgg aacgaacgcc
     7801 tgcgatccac tctcggcgtc tttttgtggg agagcgatta cacgctgcag cagaacaccg
     7861 gcctcttcaa catcttcaat cccacgagcg ttacaaactt caccgcctcc cccttctacc
     7921 aacaggacgt ggactcgatg ggagtcttcg gacaggtcga ttgggacatt accgatcgtc
     7981 tgacgctcag cctcggcgga aggtggctcg acgaggagaa gtcggcctgc atgacggtga
     8041 cgggctattc cgaaaacccg ttccaacaga tcctggatct gcccccgggt gaggtggttt
     8101 acgtcgacgt cgcgggctac gaaaagacgc tcgatcccta cccgctgttt ccaggcccgg
     8161 gagacttgac agccggatcg tggggcgaag actgtcccga gtgggccgca ccggtatacg
     8221 accccacctt tgacggcaag gaatcctgga gcgagttcac cccaagagtc ggcgcgcaat
     8281 acaacttcga tgcgggtatg gcctacgtga cctattccga aggattccgt tcgggaggct
     8341 tcaacgggcg ggcaacggcg cctgacaata cgggccccta cgatccggaa tccgtggaaa
     8401 gctgggaaat cggcggcaag ctgatgttcc tggac
//
LOCUS       DBNHLM010000271        10097 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_757342, whole genome shotgun sequence.
ACCESSION   DBNHLM010000271 DBNHLM010000000
VERSION     DBNHLM010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10097
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_757342"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..371)
                     /locus_tag="AC16QF_12575"
     CDS             complement(156..371)
                     /locus_tag="AC16QF_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449441.1"
                     /translation="MTDIPDVGAQDPELKNSPIALESDEETVLLREEIPDEPFCYFNS
                     EAFPNGAVVKSGPMKLRCDLGIWVPIG"
     gene            542..1336
                     /locus_tag="AC16QF_12580"
     CDS             542..1336
                     /locus_tag="AC16QF_12580"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR6449442.1"
                     /translation="MKTQRLFLLIMVCALSACATSPMGRSQLILFPDSEMDQMGVAAY
                     REMQQQTPREPTAGVNDYVQCVVEHVVLAMPDGRMADWEVTVFADDQANAFALPGGKI
                     GVYTGLLDVAENQDQLATVIAHEVAHVLARHSNERVSTTYATQTGLQVVQIAGGTSSP
                     ARNQLYGLLGLGAQVGVLLPFSRAQESEADLMGLDLMAEAGFDPRESVRLWENMAESG
                     GARPPEFLSTHPSGETRMRDLNERMRRAMDLSLQARSAGRNPDCRR"
     gene            1391..2185
                     /locus_tag="AC16QF_12585"
     CDS             1391..2185
                     /locus_tag="AC16QF_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HIR6449443.1"
                     /translation="MIVEQIWTGNSYRNFNYLIVCPESGEALAIDPLDHKKCLARARD
                     KGWQITQVLNTHEHPDHTGGNGAVIEATGAKLLAHANAQRSIKGMDVALNAGDVVKVG
                     RTVELLALDTPGHTMAHVCLLSQTETPALFCGDTLFNAGAGNCHGGGHPDELYQTFAS
                     QLDQLPETTLVYPGHDYITNNLAFTLDREPDNEAAAALLKELEANHDPDAALVTSMAQ
                     EKEINTFFRLTSRTVIDRLREAFPDLPDEPTPHEVFVKLRELRNSW"
     gene            complement(2193..3068)
                     /locus_tag="AC16QF_12590"
     CDS             complement(2193..3068)
                     /locus_tag="AC16QF_12590"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6449444.1"
                     /translation="MGMLDGKVALVTGAGGGLGRAHALLLAQHGAAVVVNDLGGARDG
                     SGEGHAMADQVVAEIEAAGGRAAADYGSVSDPAAAKQMVQIAVDRFGKIDICINNAGI
                     LRDKSFKNMTDDMWDVVVDVHLRGTYLVAKAAWDQMIEQGTGGRIINTSSTSGLIGNF
                     GQANYGAAKAGIAGLTRVLALEGMKYGITVNTLAPAAWSRLTEDIMPEGMEERLAPEK
                     VSPAVVWLCTDDAANVTGRQFCAGQNRVSLLSWQVTEIADKNAMDAPWTVEEIGERMQ
                     ATMDAWPKLLKPMEV"
     gene            3206..4426
                     /locus_tag="AC16QF_12595"
     CDS             3206..4426
                     /locus_tag="AC16QF_12595"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6449445.1"
                     /translation="MNEPSGPLADITIIDCTMALAGPFGAAILADLGANVIKVEPPGG
                     DVARSVPPLPEDYANANSDQSAGCDFGGYFASVNRNKRSVVIDLKTPAGRETLLALCD
                     KADAIIENMRTGVMDRLGLGYEVLRARNPALVYGCIRGFGDPRTGRSPYAEWPAYDIV
                     AQSMGGIAHITGPESSHGFPAGASIGDLYPGTLLALGVVAAVHHARRTGEGQFMDVGM
                     YDAVMFLSETVIANFGYAGTELGPRGAHHPNLCPFGIFPTSDGAVAIAAPGPGHWQAL
                     CQAIGRPDLVDDEKTRNTFVRRKHQHFVEGVVSDWTATRTRAEVVAALGGRVPCGPVN
                     TAADIFADPHVAARGMVSQVELPGENGEVSIVGTPIKFTGTPTGLYRRPPMLGEHTEE
                     VLREMGLKGAANDE"
     gene            4457..5212
                     /locus_tag="AC16QF_12600"
     CDS             4457..5212
                     /locus_tag="AC16QF_12600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6449446.1"
                     /translation="MRLGALLGPIVDASEPNALADQARRYAGEGFESLWSAQAIGRGA
                     MYTDPFVALSVAAAVTEKVELGTAVVQVPLYHPVDLAHRALSLRQVAGERVLLGVGAG
                     STQTDFDAFGRDYASRFRTFGESVEVLRQLFEEGRAGETNLSPWPAVLGGLPLLLGSW
                     GKGVERAAASFDGWIASAHYRTPDQVVEALDGYRAAGGRRAVVSTILLGAGTDLGETG
                     DLLGRFAEAGFDDAVVMLLPGGPSAADVRRLVA"
     gene            5300..6511
                     /locus_tag="AC16QF_12605"
     CDS             5300..6511
                     /locus_tag="AC16QF_12605"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6449447.1"
                     /translation="MGLLFQAVVFGIGFFCFTFWVAPWRAEFGSSRGEVMAILVTVQI
                     VMGCAAPFAGRAMDHLSIRALVVAGAVCFAAALALAAHATALWQIGLIYSVLFVAGAL
                     LAGPLAAQTLAAKWFFARRGTAIGLSSVGTSLGGFVLPLLVTWLIAAYGWRQANLVLA
                     ALVVVVVVPVVWLVVSNTPGDRGVAPEPEASRPDGTSPVAFPQWTTRTILSERAFWVA
                     VIAFAPMMAAFGAAQQNFAPYTADLGIDARATSYLVSLMAIVMVAGKVFFGAMSDRLD
                     HRLLYWIAVGVLIVSLVLMTRTPAYGLLVVISGLLGFAAGGFLPLLGAVVGSRFGPAA
                     FGRVMGLLGPFTTVAAIGPWIAGNLRDASGTYDDAWMLFVGIMVPAALAMAFMTPRPQ
                     PLALASAERAS"
     gene            6594..7427
                     /locus_tag="AC16QF_12610"
     CDS             6594..7427
                     /locus_tag="AC16QF_12610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6449448.1"
                     /translation="MRIGVSLPVRELENDLGAIRAFAQLAEHLGLTHLRVPDQVLRQG
                     NKHLHEPLTLLAWIAGATERIELVPSVIVLPSRQTALVAKQAAEIDVLSNGRLRLGIG
                     VGGSPDEYAAMGMDYTTRGARCEEQMKLLRLLWTRKIVSFDGRWDKVNEAGLNPLPVQ
                     RPIPLWIGARGVPAQRILHRIGRLADGWFVLCTPEELPALDGAIAGAAREAGRDPGEI
                     GTEAGVAVVGPREAEWQDRVVGWRQAGLTHLCLRTLGGGLSPAEHMSKLEEVVDRLRE
                     L"
     gene            8200..9051
                     /gene="cysD"
                     /locus_tag="AC16QF_12615"
     CDS             8200..9051
                     /gene="cysD"
                     /locus_tag="AC16QF_12615"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HIR6449449.1"
                     /translation="MREVAAQAENPVMLYSVGKDSSVMLHLARKAFYPSVPPFPLMHV
                     DTRWKFKAMYDFRDQMARETGMELIVYINPEGVEKDINPFDHGSALHTDVMKTQGLKQ
                     ALDLHGFDVAFGGARRDEEKSRAKERVFSFRTSSHRWDPKNQRPELWNLYNGYKSPGE
                     SIRVFPLSNWTELDIWEYIYRERIPIVPLYFAAERPVVERDGMLIMVDDDRMRLFPGE
                     KIQTRRVRFRTLGCYPLTGAIDSTASDLSAIVLELLQSRTSERQGRAIDTDSSGSMEK
                     KKQEGYF"
     gene            9051..>10097
                     /locus_tag="AC16QF_12620"
     CDS             9051..>10097
                     /locus_tag="AC16QF_12620"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008049148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HIR6449450.1"
                     /translation="MAEAEVIQEDLQERVDAYIANQARLDLLRFITCGSVDDGKSTLI
                     GRMLYEAQLIFEDQVTSLKADSKRQGTQGGDIDFALLVDGLAAEREQGITIDVAYRFF
                     NTDRRKFIVADTPGHEQYTRNMVTGASTADVAVILVDASQGVLTQTRRHSFITSLLGI
                     RHVVLAVNKMDLVDYDRETFETITAAYEQFAEQLAFESITAIPLSALAGDNVIERSQR
                     TSWYSGPTLLGFLETVEVGRSQREHDLRFPVQWVNRPHSEFRGYSGTVVAGAVAVGDS
                     VRVLPSGEVAQVDGIVLHEDSLDQAVADQAVTITLDRELDVSRGDVIVAADAPCEVSD
                     QFDASLVWMDQETGF"
BASE COUNT         1616 a         3271 c         3398 g         1812 t
ORIGIN      
        1 cggcggatcg gtgtgcaaag tatctcaaat ttgcgacgac agcaaccgat ctgcggcctt
       61 gtcggactcc taaccgccta atcttccggg tttttttcga gtcgcttcag ccgatcggcg
      121 gcgctccatg cgccggtctc tcgcgcttcg cgtcttcagc cgatcggcac ccagatcccg
      181 agatcgcagc gcagcttcat cgggccgctc tttaccacgg cgccgtttgg aaatgcttcg
      241 gaattgaagt agcagaaggg ctcgtccggg atctcttctc tgagcagcac ggtttcctcg
      301 tcgctctcca gcgcaattgg cgagttcttg agctcggggt cctgagcgcc cacgtccggt
      361 atgtcggtca ttggaatgtg tcctgttggc tttcgagtca tgctagcgga cggaacgaat
      421 gcaatggcat ccggtcaacc ccgtagatgg acattctaca atgcccggag agggtggcgc
      481 gcgtgttgtc tctctcgggc agttcgcgca tcctttccgc gggccacatg gggatcatcc
      541 cgtgaagaca caacgactct ttctcctgat catggtttgc gcgctgtccg cctgcgcgac
      601 ctcgcccatg gggcgcagtc agctcatcct gtttccggat tcggaaatgg accagatggg
      661 ggtagccgcc taccgggaga tgcagcagca gacgccccgc gagccgacgg ccggcgtcaa
      721 cgactacgtt cagtgcgtgg tcgagcacgt ggtgctggcc atgcccgacg gcaggatggc
      781 cgactgggag gtcaccgtct ttgccgatga tcaggccaac gccttcgccc ttccgggggg
      841 caagatcggc gtctacaccg gcttgctcga tgtggccgaa aaccaggacc agctcgccac
      901 cgtgatcgcg cacgaagtgg ctcacgtgct ggcgcgccac tcgaacgagc gggtatcgac
      961 cacctacgcc acccagacgg gcctgcaggt cgtgcagatc gccggcggga cgagctctcc
     1021 cgcgcgcaat cagctgtacg ggcttctagg gctcggcgct caggtcggcg tgctcctgcc
     1081 gttcagccgg gcccaggaga gcgaggcgga cctgatgggt ctcgacctca tggccgaggc
     1141 aggcttcgat ccccgcgaga gcgtgaggct ctgggagaac atggcggaat ccgggggcgc
     1201 ccggccgccg gagtttctct cgacgcaccc ctccggggag acgcgcatgc gtgacctcaa
     1261 cgagcgtatg cgccgcgcca tggacctctc cctgcaggca cgctcggcgg ggcgcaaccc
     1321 cgactgtcgt cggtaagtcc cgggcgcgtg cgtgggttac actgacgctt tcgactaaac
     1381 gggcacgcgc atgatcgtcg agcagatctg gaccggtaac tcctaccgaa acttcaacta
     1441 cctcatcgtc tgtccggagt cgggcgaggc gctggccatc gaccctctgg accacaagaa
     1501 atgcctggcc cgcgcgcggg acaaggggtg gcagatcacc caggttctca atacccacga
     1561 gcacccggat cacaccggcg gcaacggtgc ggtgatcgag gccaccgggg caaagctgct
     1621 ggcccacgcc aacgcccagc gcagcatcaa gggcatggac gtcgcgctca atgcgggcga
     1681 cgtggtcaag gtgggtagaa ccgtggagct gcttgcgctg gatacgccgg gccacacaat
     1741 ggcccacgtc tgcctgctgt cccagacaga gacgccggcc ctgttctgtg gcgacaccct
     1801 gttcaacgcc ggggccggca actgtcatgg cggggggcat cccgacgagc tctaccagac
     1861 gttcgcctca cagctcgatc agctgccgga aaccaccctc gtgtatccgg gacatgacta
     1921 catcaccaac aacctggcct tcaccctgga ccgggagccc gacaacgagg ccgcggcagc
     1981 gcttctgaag gagctggagg cgaatcacga cccggacgcc gctctcgtga cgtccatggc
     2041 ccaggaaaag gagataaaca cctttttccg gctgacgagc cgcacggtca tcgaccgcct
     2101 gcgggaggcg tttccggacc tgccggacga gcccacccct catgaggtgt tcgtgaagct
     2161 ccgcgagctc aggaactcct ggtagccgcg gcctagacct ccatgggctt gagcagcttg
     2221 ggccaggcgt ccatggtggc ctgcatgcgc tcgccgatct cctcaaccgt ccacggcgcg
     2281 tccatggcgt tcttgtcggc gatctcggtc acctgccagg agagcagcga gacccggttc
     2341 tgcccggcgc agaactgacg gccggtaacg ttggctgcgt cgtcggtgca cagccagacc
     2401 acggccggag agactttctc cggggccagc cgttcctcca tgccttcggg catgatgtcc
     2461 tcggtcaggc gtgaccaggc ggccggggcc agggtgttga cggttatgcc gtatttcatc
     2521 ccctccagcg ccagcacccg ggtgagcccg gcgatgcccg ccttggcggc gccgtagttg
     2581 gcctgaccga agttgccgat gagcccggag gtggagctgg tgttgatgat ccggccgccg
     2641 gttccctgct cgatcatctg gtcccaggcc gccttggcga ccaggtaggt cccgcgcagg
     2701 tgaacgtcca ccacgacgtc ccacatgtca tcggtcatgt tcttgaacga tttgtctctc
     2761 aaaatgccgg cgttgttgat gcagatgtcg atcttgccga atcggtccac ggcaatctga
     2821 accatctgct tcgctgcggc cggatcgctc accgacccgt agtcggctgc ggccctgccg
     2881 cccgcagcct caatctcggc caccacctgg tcggccatgg cgtgcccctc tccggagccg
     2941 tccctggcgc cgcccagatc gttgaccacc accgcagcgc cgtgctgcgc cagcagcagg
     3001 gcgtgggcgc ggccaagccc tccaccggct ccggtgacca gcgctacctt gccatccaac
     3061 atgcccatgt tcttcccccg gcggtttcgc ctgtttgtcg agatcgaaaa ttctagctaa
     3121 aagcggggtc gcccggccac gggcgcttcg aggtgtgccg gcccgatcgg tgcgggtata
     3181 gttgctccgg gaggagagat ggcgcatgaa cgagccctct gggccgctgg ccgatatcac
     3241 catcatcgat tgcaccatgg cgctggcggg gccgtttggt gcggcgattc ttgccgacct
     3301 cggcgccaac gtcatcaagg tggagccgcc gggcggggat gtggcgcgaa gcgtgccgcc
     3361 gctgcccgag gattacgcca acgcaaactc ggaccagtcg gccggctgcg atttcggcgg
     3421 ctacttcgcc agcgtcaacc gcaacaagcg cagtgtggtc attgatctga agaccccggc
     3481 ggggcgcgag accctcctgg cgttgtgcga caaggcggat gccatcatcg agaacatgcg
     3541 aaccggggtg atggatcgcc tcgggctcgg ctacgaggtg ctgcgggcgc gcaacccggc
     3601 cctggtctat ggctgcatcc ggggtttcgg ggatccgcgc acgggccgca gtccatacgc
     3661 cgagtggccc gcctacgaca tcgtggcgca gagcatgggc ggcatcgcgc acatcacggg
     3721 gcctgagagc tctcacgggt ttccggctgg cgccagcata ggcgatctct atccggggac
     3781 tctgctcgcc ctgggagtgg tcgcggccgt ccaccatgcc agacggacgg gcgaagggca
     3841 gttcatggac gtggggatgt acgatgccgt gatgtttctc tccgagacgg tgatagccaa
     3901 cttcggctac gccggcacgg agcttggccc ccggggcgcg catcatccca acctctgccc
     3961 gttcggcatt ttcccgacct cggacggcgc ggtggccatc gccgctcctg gtccgggaca
     4021 ctggcaggcg ctctgtcagg ccatcggccg gcccgacctc gtggacgacg agaagacgcg
     4081 caacacgttc gtgcggcgca aacaccagca tttcgtggag ggcgttgtgt cagactggac
     4141 ggcgacgcgc accagggccg aggtggtggc cgcgctcggg ggcagggtgc cgtgcgggcc
     4201 ggtcaacacc gctgcggaca tcttcgccga cccccacgtg gcggcgcgcg gcatggtcag
     4261 ccaggtggaa ctgccgggag aaaacgggga agtttccatc gtgggcaccc ccatcaagtt
     4321 cacgggtacc cccaccggcc tctaccgccg accgcccatg ctgggcgagc atacggaaga
     4381 ggtgcttcga gaaatgggct taaaaggtgc cgccaacgat gagtgagcga tcaattcgaa
     4441 tgaaggagga gttgtcatga ggcttggggc attgctggga ccaatcgtcg acgcgtccga
     4501 gcccaatgcg ctggcggatc aggcgcgccg atacgctggc gagggtttcg aaagtctctg
     4561 gtcggcccag gccatcgggc gtggcgccat gtacacggac cccttcgtcg cgctttccgt
     4621 ggcggcggcg gtgaccgaaa aggtggagct cggcactgcg gtggttcagg tgccccttta
     4681 ccacccggtc gatctggctc accgcgcgct ttcgctgcgg caggtagccg gcgagcgtgt
     4741 gctgctcggg gtgggggcgg ggtcgaccca gacggacttc gacgccttcg gccgggacta
     4801 cgcttcccgc ttccggacgt ttggcgagtc ggtcgaggtg ctcaggcagc tcttcgagga
     4861 agggcgtgcg ggggagacca atctctctcc ctggccggcc gtgctcggcg ggctgccgct
     4921 gctgctgggc tcgtggggca agggcgtcga gcgagcggcc gcgtcatttg acggctggat
     4981 cgcttctgcc cactaccgca ccccggacca ggtggtggag gcgttggatg gctaccgcgc
     5041 cgccggtgga aggcgcgccg tggtgtccac catcctgctc ggagccggca ccgacctggg
     5101 cgaaaccggc gacctgctcg gccgctttgc ggaggctgga ttcgatgacg ccgtggtcat
     5161 gctcctgccc ggcgggccat ccgccgcgga cgtccggcgc ctggtcgcct gacccaggcc
     5221 ccggggagcc cgcaacatcg acggcacgcc gcgagcacga accgaacccc gctggtacta
     5281 cggctggaac gtgctgggcg tggggctttt gttccaggcc gtggtcttcg gcatcggctt
     5341 tttctgcttc accttctggg tggcgccgtg gcgcgcggag ttcggcagca gccggggcga
     5401 ggtcatggcc atcctggtca ccgttcagat cgtgatgggc tgcgcggcac ccttcgcggg
     5461 ccgcgccatg gaccacctgt cgatccgggc tctggtggtt gcgggcgcgg tctgtttcgc
     5521 tgcggccctg gcgcttgccg ctcacgccac cgcgctgtgg cagatcggcc tcatctattc
     5581 ggttctgttc gttgccggcg cgctgctggc gggaccgctg gcggctcaga ccctggcggc
     5641 caagtggttc ttcgcccgtc ggggcacggc catcggcctg tcgtcggtgg ggacctccct
     5701 gggcggcttc gtgctcccgc tgctggtgac ctggctcata gccgcctacg ggtggcgcca
     5761 ggccaacctg gtcctggctg ccctggtggt ggtcgtggtg gtgcccgtgg tctggctggt
     5821 ggtgagtaac acgccgggcg atcgcggcgt tgccccggaa ccggaggcat ctcggcccga
     5881 cggcaccagc ccggtggcgt tcccccaatg gacgacgcgc accattctct cggaacgggc
     5941 cttctgggtg gccgtcatcg cgttcgcgcc gatgatggcg gcatttggcg ccgcccagca
     6001 gaatttcgcg ccctacacgg ccgatctcgg catcgacgcc cgagccacgt cataccttgt
     6061 ctcgctcatg gccatcgtga tggtggccgg caaggtgttc ttcggcgcca tgtccgatcg
     6121 cctggatcat cgcctgctct actggatcgc cgtgggtgtg ctcatcgtct cgctggtgct
     6181 catgacccgg accccggcgt acgggctgct ggtggtgatc agtggcctgc tgggattcgc
     6241 ggcgggcggt ttcctgccgc tgctcggcgc ggtggtgggc agccgcttcg ggccggccgc
     6301 gttcggtcgg gtcatgggcc tgcttgggcc gttcaccacc gttgctgcca tcggtccctg
     6361 gatcgccggg aatctgcgcg acgcctcggg aacctacgat gacgcctgga tgctcttcgt
     6421 cggcatcatg gttccggcgg ctctggccat ggcgttcatg accccgcgcc ctcagccgct
     6481 cgctcttgca agcgctgaac gggcgtcgtg aaagtgatgg acacaacagg ctgactcatc
     6541 tcgaaaactg accctctcgg cgtcgcgagg gagaactcta agggaggatc cacatgcgaa
     6601 tcggcgtatc gctacccgtg cgggagcttg aaaacgatct gggtgctatc agggcgttcg
     6661 cgcagctggc cgagcacctc gggctgaccc atctccgggt acccgaccag gtgctgcgtc
     6721 agggtaacaa gcatctgcac gagcccctga cgcttctcgc ctggatagcc ggcgcgaccg
     6781 aacgcatcga gctcgtgccg tcggtcatcg tcctgccgtc gcggcagacg gctttggttg
     6841 ccaagcaggc cgcggagatc gacgtgctct ctaacggccg gttgcggctg ggcatcggcg
     6901 tcggcggcag cccggacgag tacgcggcca tggggatgga ctacacgacg cgtggcgccc
     6961 gctgcgagga gcagatgaag ctgctgcgac tgctgtggac ccggaagatc gtgagcttcg
     7021 acgggcgctg ggacaaggtc aacgaggcgg gcttgaaccc tttgcccgtg cagcgcccga
     7081 ttcccctgtg gatcggcgcc cgcggggttc cagcgcagcg gattcttcac cgcatcggcc
     7141 ggcttgccga tggctggttc gtgctgtgca cgcccgagga gcttcccgcg ctcgacggcg
     7201 ccatcgccgg ggcggcacgg gaagcgggac gtgatcctgg ggagatcgga acggaggcgg
     7261 gtgtggctgt ggtaggaccg cgggaggccg agtggcagga ccgggttgtg gggtggcggc
     7321 aggccgggct gacgcacctc tgcctgcgga ccctgggcgg cgggctgtcg ccggcagagc
     7381 acatgagcaa gctggaagaa gtggtcgatc ggttgcggga gctttgaggc ccgctgttgg
     7441 gtgtcgcaat atgcagattt gtacgtattg gttacattaa tccgtgtcta attcgttgca
     7501 ggcgcgctga tttcgaatat tattgcgcat cacgacattc gaggtgcgcg cccagtggag
     7561 ctacccgcca gcctttacaa gaaccggtcc caggcccagg gtgcctggga tacggccgcc
     7621 gtccacgagc tgctcagcga atcgggcagc gctcagtctc tcaggtcgac ggagcgtctt
     7681 ctggaaagcg ccgacctgct gcccgccacg ctcaagctgg acgaggtgcg cgacgagctc
     7741 ggccgcgagc cggtccggct ggtggtggat gctctggaag ccgaagccaa gcgtaagcgt
     7801 catctctccc tgattctcca ggctcacgag gttcccgggg cggggctcat gctcttcgcc
     7861 aacgacgggc gcatgggcag acgctggctc tacctggatg gcgggcggct ggatgtcgat
     7921 gggctcctga acgcgctgga cgtgcttgcc ggcggtcggg aagcgcccaa gcagctgctg
     7981 atctggcccc atggggaggt agcgcgcgcc tgtcgggcgc tggtggaacg ggcagactcc
     8041 cacgatatcg agctgccttc cggcggcaag gcgcggatgg ggacgctggc ctatcgtgcc
     8101 gccgaaagcg tcgctcgcca taatgactcg ccgccccgtc ggcacccggg gcaggagctt
     8161 tcacacctgg accggctgga ggcggaaagc atccacatca tgcgggaagt cgccgcccag
     8221 gcggagaacc cggtgatgct gtactcggtg ggcaaggaca gctcggtgat gctgcacctc
     8281 gcccgtaagg ccttctatcc cagcgtaccg ccgtttccgc tcatgcacgt ggacacgcgc
     8341 tggaagttta aggccatgta cgacttccgc gaccagatgg cccgggagac gggcatggag
     8401 ctgatcgtct acatcaaccc cgaaggggtg gagaaagaca tcaacccctt cgatcacggc
     8461 agcgctctgc acaccgacgt gatgaaaacc cagggtctca agcaggcgct ggatctccac
     8521 ggcttcgacg tcgcctttgg cggcgcccgc cgggacgagg agaagagccg ggcgaaggag
     8581 cgcgtgttct cgttccgcac gtcgagccac cgctgggatc cgaagaacca gcgccctgag
     8641 ctgtggaacc tgtacaacgg ctacaaaagc ccgggcgaga gtatccgggt gtttcccctg
     8701 tcgaactgga ccgagctcga catctgggaa tacatctacc gtgagcgcat ccccatcgtt
     8761 cccctctact tcgcggccga gcgccccgtc gtcgagcgcg acggcatgct gatcatggtg
     8821 gacgacgacc gcatgcggtt gttccccggc gagaagattc agacccggcg ggtgcgcttt
     8881 cgaaccctcg gctgctaccc gctcacgggc gccatcgatt ccacggcaag cgacctgtcc
     8941 gccatcgtgc tcgagctcct gcagagccga accagtgagc gccagggccg ggccatcgat
     9001 acggactcct ccgggtccat ggagaagaag aaacaggagg gctatttctg atggccgaag
     9061 cagaagttat tcaggaagac ctgcaggagc gggtcgacgc ctacatcgca aaccaggcgc
     9121 gcctcgatct gctccgcttc atcacctgcg gcagcgtcga cgacggcaag agcacgctca
     9181 tcggccgcat gctctacgag gcccagctca tcttcgagga tcaggtgacc tcgctgaaag
     9241 ccgactccaa gcgccagggg acccagggcg gcgacatcga cttcgccctg ctggtggacg
     9301 gccttgcggc ggagcgggag cagggcatca ccatcgacgt ggcctaccgc ttcttcaata
     9361 ccgaccggcg caagttcatc gtggcggaca cgccgggcca cgagcagtac acgcgcaaca
     9421 tggtcaccgg tgcttccacc gcggacgtcg ccgtgatcct ggtggatgcc tcccagggcg
     9481 tgctcaccca gacccggcgc cactccttca tcacctcgct cctcgggatc cggcacgtgg
     9541 tgctggccgt gaacaagatg gacctcgtgg actacgaccg ggagaccttc gaaaccatca
     9601 ccgcagcata cgagcagttt gcggagcagc ttgccttcga gtcgatcacc gccattccgc
     9661 tgtccgcgct tgcgggcgac aacgtgatcg agcggtcgca gcggacgtcc tggtacagcg
     9721 gcccgacgct gctgggtttt ctggaaaccg tggaagtggg gcgctctcag cgtgagcacg
     9781 acctgcgttt tcccgtgcag tgggtgaacc ggccgcactc ggagtttcgc ggttactccg
     9841 gcacggtggt ggcgggcgcg gtggccgtcg gcgactccgt gcgggtgctg ccatccggag
     9901 aggtggcaca ggtcgacggc atcgtcctgc acgaggattc cctggaccag gccgtggccg
     9961 atcaggccgt gaccattact ctggaccggg agctcgacgt ttcccggggc gacgtgatcg
    10021 tggccgcgga tgccccatgc gaggtctcgg atcagttcga tgcgtccctg gtctggatgg
    10081 atcaggaaac gggattc
//
LOCUS       DBNHLM010000272         7960 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1356961, whole genome shotgun sequence.
ACCESSION   DBNHLM010000272 DBNHLM010000000
VERSION     DBNHLM010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7960)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1356961"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="AC16QF_12625"
     CDS             <1..324
                     /locus_tag="AC16QF_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6449451.1"
                     /translation="DDAEPKTQVGKILQTLEPDPHRYRILLYHRPDGARDAAAWGVNL
                     MLCGHTHAGQIVPFNLLVKRVFPRIRGVYRVDGLTLYVSPGTGTWGPILRLGSRSEIS
                     MMTLG"
     gene            347..1027
                     /locus_tag="AC16QF_12630"
     CDS             347..1027
                     /locus_tag="AC16QF_12630"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6449452.1"
                     /translation="MAEEQELIVEQIEIGPMQNFTYIVGSRSAREVAIVDPAWDVEHL
                     VRHIEEKGYKLTAALVTHYHPDHIGGSFGGHNIDGIAELLAINPLKVYAHKEEAEGVR
                     KVTGISESDLVRVESGDDLRIGDVSIEFLHTPGHTPGSQCFRVHNTLVSGDTLFIQGC
                     GRVDLPGSNSEDMYHSLQKLKSLPDETLLLPGHNYAHVPNATMGETKKFNTYMHVKDL
                     QTWRMIMG"
     gene            1165..1416
                     /locus_tag="AC16QF_12635"
     CDS             1165..1416
                     /locus_tag="AC16QF_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449453.1"
                     /translation="MNAYDHLVGQHFLRAGVRYTVVKCVGSTAITAYLRDNRIYRVVM
                     RVSDVLDLLEVTEISITVPAMEPKKALPAPQLERASGQS"
     gene            1572..1922
                     /locus_tag="AC16QF_12640"
     CDS             1572..1922
                     /locus_tag="AC16QF_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449454.1"
                     /translation="MREQPVFETQGLWVRWSWDEKRKSLKLEKAEPRPQPATAQPTSH
                     WIFTLRDQLVSRSSRLLLRVGVWVLRQRMSSAEVGEYLRETADRVASGHHEPGDPLES
                     HARRCNWNHFFVAK"
     gene            complement(1967..2941)
                     /locus_tag="AC16QF_12645"
     CDS             complement(1967..2941)
                     /locus_tag="AC16QF_12645"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR6449455.1"
                     /translation="MKDPVRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQLLEIT
                     PALNALQGVVMELEDCAFALLHGVVATDDPNVAFKDATHALLVGSRPRSKGMERKDLI
                     EANAAIFSVQGKALNDHADRAVKVLVVGNPANTNCLIAQRNAPDLDPRQFTAMTRLDH
                     NRAAAQLAARTGKRVTDVAGLCIWGNHSATQYPDLHAATVAGTPAMELVDMAWYTGEF
                     IPTVQQRGAAIIEARGASSAASAANAAIDHMHDWSLGSEGVVSMGVYADGSYGVEPGL
                     IYSFPVTCEGGDWQIVQGQEIGEFSREKMAATEKELVEERDAVAHLLS"
     gene            complement(3056..3487)
                     /locus_tag="AC16QF_12650"
     CDS             complement(3056..3487)
                     /locus_tag="AC16QF_12650"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR6449456.1"
                     /translation="MERDRFPVVVHVLMFRGDELFLLRRASTGFMDGYYALPGGHQRA
                     GESVAQAARRECEEETGVTDLDLAPICVMPYRAGRHQGLNFVFEAASWLGEPRVAEPE
                     LFSEGVWAAARDLPEPHAMWISDALQCRDEGDWFREFLWHG"
     gene            3556..4320
                     /locus_tag="AC16QF_12655"
     CDS             3556..4320
                     /locus_tag="AC16QF_12655"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF049302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6449457.1"
                     /translation="MTRAGKPDAALTRRIEEASLNAWPAMEQLLMDGWLLRFSRGFTK
                     RANSVVPLYPWTGNGREPLAERVRHCENLYAREGLQTIFRLPAIDDNTALDAHLESRG
                     YNLVDPTLVLVAPTRPGPGHEDFALVPAEHWLDIYAELTGMPDTARKLHGAIVAGIPT
                     PCGFAVLRRGERPVACGLAVVERDLLGLFDIVTDPDARRSGHGTALVGSLLGWGHARG
                     ARLAYLQMIESNAPASALYRKLGFEIAYNYWYRISP"
     gene            4604..>7960
                     /gene="gltB"
                     /locus_tag="AC16QF_12660"
     CDS             4604..>7960
                     /gene="gltB"
                     /locus_tag="AC16QF_12660"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HIR6449458.1"
                     /translation="MSIRTPWGFPPKRGLYDPANEKDSCGVGFICDIKGRASRDIIDQ
                     AQHMNCCMTHRGGVGYERNTGDGAGILTGLPDKFLRRVAASELGVTLPPQGQYGVGNV
                     FLPNDEAERRHCIAIFEREIQAAGQKLLGWRELPVHPDGAGIGHAARAAMPTFAQLFV
                     GAKDASGDAFERKLYLIRKHSTHLLRGDMALGERKLFYVCSLSSKVIIYKGMLTPEQV
                     FPFYQDLDDPDYESHLAMVHSRFSTNTFPSWDRAQPNRFMSHNGEINTLLGNVNSMNA
                     RQGLLSSPLFGADIEKLYPIVEEDCSDSGTFDNVLEFLLMSGRKLQEAVLMMIPEAWQ
                     QHPTMDQAKRAFYEYHSCLMEPWDGPASIAFTDGTYIGAVLDRNGLRPSRYYITNDDR
                     CIMASEVGVVPVDPACVLEKGRLQPGRIFLIDFEQGRMIPDAEVKQEWVKARPWQQWL
                     DNQRIDLAEIPPGDDPHGFHPANLTPRMQAFGYTVETMEIMLQPLVKELRDPLGSMGN
                     DAALAVMSDKPRMIYDYFKQRFAQVTNPAIDSIREEVIMALECYIGPERNLLETTEEH
                     AHRLRVPHPILSNEELAALKHMDHRGWRSRTIDITFKQGTGRDGLVAALDRICAEASG
                     AIADGYSLVVLSDRGIGPDRVPVSSLLAVGAVHHHLVREQSRTRIGIVLETGEAREVH
                     HHCLLVGFGADAINPYLAFEALWQARRNGYLDDAAHITSDDDVVAAYRKGTAKGMLKV
                     MAKMGISTLHSYKGAQIFEAVGLADDVIDVAFVGTASRVQGIGLRVLAEEMERRHVIG
                     YSKRSQNVTVLPNPGDFHWRRTGDTHMWDPSAIASLQVAVRGNSEDAYWQFARHVNEE
                     TTRHANLRGLLTFNKDVNGGPIPIEEVEPATEIVKRFATGAMSFGSISAEAHESLAIA
                     MNRLGGKSNTGEGGEDPSRFIPLENGDSKRSAIKQVASGRFGVTINYLTNADELQIKI
                     IQGAKPGEGGELPGHKVDDIIANIRHSTPGVGLISPPPHHDIYSIEDMAQLIHDLKNA
                     NPEARISVKLGAEVGVGTVAAGVTKARADHLVIAGDTGGTGASPLTSIKHAGVPWELG
                     IAETHQTLVVNNLRSRVVLQTDGQLKTGRDV"
BASE COUNT         1519 a         2729 c         2399 g         1313 t
ORIGIN      
        1 gacgatgccg agccgaagac ccaggtcggc aagatcctgc agacgctcga gcccgaccct
       61 cacaggtacc ggatactgct gtatcaccgt ccggacgggg cgcgggacgc ggcggcctgg
      121 ggcgtcaacc tcatgctctg cgggcacacc cacgcggggc agatcgtgcc gttcaatctg
      181 ctcgtcaaac gagtattccc acgcatccgg ggcgtttacc gggtggatgg tttgaccctt
      241 tacgtctctc cgggcaccgg aacgtggggc cccattctcc gcttaggatc tcgttcagag
      301 atcagtatga tgacccttgg ctgaagaaac gaccggacaa gcaatcatgg cagaagagca
      361 ggaactcatc gtcgagcaga tcgagatcgg gcccatgcag aacttcacgt acatcgtggg
      421 gtctcgatcg gcgcgcgagg tggccatcgt ggacccggca tgggacgtgg agcacctcgt
      481 gcgccacatc gaggaaaagg gctacaagct caccgcagcg ctggtgactc actaccatcc
      541 cgatcacatc ggcgggtcct ttggcgggca caacatcgac ggcatcgccg agctgctcgc
      601 catcaatcct ctcaaggtct acgcgcacaa ggaggaagcc gaaggagttc gcaaggtcac
      661 gggcatatcc gagtccgatc tcgttcgcgt ggagtccggc gacgatctgc gcatcggaga
      721 cgtgagcatc gagttcctgc acacgcccgg gcacacgccc ggctcccagt gctttcgagt
      781 tcacaacacg ctggtgtccg gcgacaccct cttcattcag ggctgcgggc gcgtggacct
      841 tcccggatcg aactccgagg acatgtacca cagccttcag aaactcaaat cgctccccga
      901 cgagaccctt cttctgccgg gacacaacta cgcccacgtc cccaacgcaa ccatgggcga
      961 gacgaaaaaa ttcaacacct acatgcacgt caaggatctg cagacctggc gcatgatcat
     1021 gggctgacgc gcctgccgcc gatcggccgg tttccgccat tcgtcatcgc cgctcgccgg
     1081 gcggccgcta aaccccgact gccggtcgtg cgtcccggcg atccgcgccg ctaagattcc
     1141 gtaggagttg aagaggaatt cgacgtgaac gcctacgatc atcttgttgg ccagcatttc
     1201 ctgcgcgccg gagtgcgcta caccgtggtc aagtgtgtcg gctcgaccgc catcaccgcc
     1261 tatcttcgcg acaaccggat ctaccgggtc gtgatgcgcg tgagcgacgt tctcgatctc
     1321 ctggaagtga cggagatcag catcaccgtt cccgcgatgg agcccaagaa agccctgccg
     1381 gcaccgcagc tcgagcgcgc atccggccag tcctgacaag gcgccgccct gggtatccca
     1441 ggccggacct ttcctcccgg ttccgccaag ggggcatgag cctgccaacg cggctagtct
     1501 caaaggccgg ttaacgaacc gcttacatca ctgcgatatc agttgagctc ggacacctat
     1561 agattgagcc gatgagagag cagccggtat ttgagacgca gggcctgtgg gtccggtgga
     1621 gctgggacga gaagcgcaag tccctgaagc tcgagaaggc ggagccacgt cctcaaccgg
     1681 ccaccgctca acctacgtcg cactggatct tcacgctccg cgaccagctc gtatcgcgca
     1741 gcagccggct gctgcttcgc gtcggggtgt gggtgctcag acaacggatg tccagcgccg
     1801 aggtcggcga gtacctgcgc gaaacggccg accgcgtcgc aagcggtcac cacgagcccg
     1861 gcgaccccct ggaaagccac gcccggcgct gcaactggaa ccacttcttc gtcgccaagt
     1921 agcggtttgc gaaagctgct gcctccgcca gcctctacag gccgtttcag ctcagcaggt
     1981 gcgctacggc gtcgcgttcc tcgacgagtt ctttctctgt cgcagccatc ttctcacggc
     2041 tgaattctcc gatctcctgg ccctgcacga tctgccagtc gccaccctca caggtgaccg
     2101 ggaaggagta gatcagcccc ggctcgacgc cgtagctgcc atccgcgtag acgcccatgc
     2161 tcaccacgcc ctcgctgccc agagaccagt catgcatgtg gtcgatggcg gcgttcgctg
     2221 ccgaagccgc gctggacgct ccgcgggcct ctatgatcgc ggccccccgc tgctgcaccg
     2281 tgggaatgaa ctcccccgtg taccacgcca tgtcgacgag ctccatggcg ggcgtgccgg
     2341 caacggtcgc cgcgtgaaga tcgggatact gcgtggccga atggttgccc cagatgcaca
     2401 gcccggcaac gtccgtcacc cgctttccgg tccttgccgc cagctgcgcg gccgcacggt
     2461 tgtgatccag ccgggtcatg gcggtgaact gccggggatc caggtcgggc gcgttgcgct
     2521 gagcgatgag acagttcgtg ttcgccgggt tgccaaccac gagcaccttc accgcgcgat
     2581 ccgcatggtc gttgagcgcc ttgccctgca cggagaatat cgccgcgttc gcttcgatga
     2641 ggtccttgcg ttccatcccc ttgcttcgcg ggcgtgagcc gaccagcagc gcatgggttg
     2701 cgtccttgaa agccacgttg ggatcgtccg tggcgacgac cccatgaagc agcgcaaacg
     2761 cacagtcttc cagctccatc accaccccct gaagcgcgtt cagcgcaggc gtgatctcca
     2821 ggagctgcag gatcaccggc tgatcctttc ccagcatctc cccggaagca attcgaaaca
     2881 gcaatgcata tccaatctga ccggcagcgc cggtaaccgc gacccgcacg ggatctttca
     2941 tgtctgactc cctttcacaa tctcgactgg ccgcgcgtgc cgatacggca tgtcgttacg
     3001 ccggcccata gctcagcggc ggcgcattgt aaggaaagca aatcgctttc gcacgtcacc
     3061 cgtgccagag aaactccctg aaccaatccc cctcgtcccg acattgcagc gcgtcggaaa
     3121 tccacatggc gtggggctcg ggaagatcac gcgcggccgc ccatacgcct tccgagaaca
     3181 gctccggctc ggcaacgcgc ggctcgccca gccaagacgc cgcctcgaag acgaagttga
     3241 ggccctgatg ccgaccggct cgatacggca tcacgcagat cggcgcgagg tccaagtcgg
     3301 ttacacccgt ctcttcctcg cactcgcgcc gagcggcttg cgcaacactc tcacccgcgc
     3361 gctgatggcc gcccggaagg gcgtagtagc cgtccatgaa cccggtgctg gcgcgccgca
     3421 ggagaaagag ctcgtcgccc ctgaacatca gcacgtgcac caccaccgga aaccgatcgc
     3481 gttccaccag acccgctcaa accccttttg atcctgctat ggtgcgcggg tgaatgatgc
     3541 cgagcaaccc cgcccatgac ccgagcaggc aagccagacg cggcgttgac ccgacggatc
     3601 gaggaggcgt cactgaacgc gtggccggcc atggagcaac tgctcatgga cggctggctg
     3661 ctccgcttct cccgcggctt caccaaaagg gcgaacagcg tcgttcccct ctacccctgg
     3721 accgggaacg ggcgcgagcc actggcagaa agggtgcgcc actgcgaaaa cctgtacgcc
     3781 cgggaaggcc tgcagaccat attccggctg ccggccatag acgacaacac cgctttggat
     3841 gcgcatctcg aaagccgcgg ctacaacctc gtcgacccga ccctggtgct cgtcgcacca
     3901 acgcggccgg gcccgggcca cgaggatttc gcgctcgttc ccgcggagca ttggctcgac
     3961 atctacgccg agctcacggg catgcccgac accgcgcgaa agctgcacgg cgcgatcgtc
     4021 gccggcattc caaccccctg cgggttcgcc gtcctgcgac gcggcgagcg cccggtcgcc
     4081 tgtggcctgg cggtcgtcga gcgtgacctg ctcggcctgt tcgacatcgt cacggaccct
     4141 gacgcccgcc gttccggtca cgggaccgcc ctggtgggtt ccctcctcgg ctggggccac
     4201 gcccgcggtg cccggctggc atatcttcag atgatcgaat ccaacgcgcc ggccagcgcg
     4261 ctctaccgaa agcttggctt cgagatcgca tataactatt ggtaccggat ttcgccctag
     4321 ccataacctt ttgttcctag gcggccgaac ttgattcata cccgcatttt tgagatgttt
     4381 tgtctttcgc gcggacacac cgcagctagc ttgccagcaa atatataaga atcagtaagt
     4441 tataaacggc aagcagatct ccgacaggac ctgcgcgctt ccgtggcgga agccgacggc
     4501 tgcggtcgcg cccgacatcc gctcccactt tgggcggcgt cggcgtctgg ctcccaccat
     4561 tgaggtcgaa gcggccgggc caaacacgaa agagaacagt tgcatgagca ttagaacgcc
     4621 atgggggttt cccccgaagc gaggtctgta cgacccggct aacgagaaag acagctgcgg
     4681 cgtcggcttc atctgcgaca tcaagggtcg cgcgagccgg gacatcatcg atcaggccca
     4741 gcacatgaac tgctgcatga cccatcgcgg cggcgtcggc tacgagcgca acacgggcga
     4801 cggggccggg atcctgacgg gcctgccaga caagtttctg cggcgcgtcg ccgccagcga
     4861 gctgggcgtc acccttcctc cccagggcca gtacggcgtg gggaacgttt tccttcccaa
     4921 cgacgaagcc gagcgccgac actgcatcgc catcttcgag cgggaaattc aggcggcggg
     4981 tcagaagctg ctcggctggc gggaactgcc cgtgcacccc gacggcgcgg gaatcggcca
     5041 cgcggcccgg gccgccatgc ccacattcgc ccagctgttc gtgggcgcca aggacgcatc
     5101 cggcgatgcg ttcgagcgca agctctacct gatccgcaag cacagcaccc atcttctgcg
     5161 tggggacatg gcgctcggtg agcgaaaact cttctacgtc tgcagcctct cctccaaggt
     5221 gatcatctac aagggcatgc tgacgcccga acaggtcttt cccttctatc aggacctgga
     5281 cgatcccgat tacgaatcgc atctggccat ggtccactcg cgtttcagca ccaatacctt
     5341 cccgtcgtgg gaccgggcac agcccaaccg gttcatgagc cacaacggcg agatcaatac
     5401 cctgctcggc aacgtcaact ccatgaacgc ccgccagggt ctgctcagca gcccgctgtt
     5461 cggcgcggac atcgagaagc tctacccgat cgtcgaggag gactgctcgg actcgggcac
     5521 cttcgacaac gtcctcgagt tcctgctgat gagcggccgc aagctgcagg aagcggtgct
     5581 catgatgatt ccggaggcct ggcagcagca cccgacgatg gatcaggcga aacgggcctt
     5641 ttacgagtat cactcctgcc tgatggagcc gtgggacggc cccgcatcca tcgccttcac
     5701 cgacgggaca tacatcggcg ccgtgctcga ccgcaacggc ctgcgcccga gccgctacta
     5761 catcaccaac gacgacaggt gcatcatggc ctccgaggtc ggcgtcgtgc ccgtcgaccc
     5821 cgcctgcgtg ctcgagaagg ggcgcctgca gcccggtcgg atcttcctca tcgacttcga
     5881 gcagggtcgc atgattccgg acgccgaggt caaacaggag tgggtgaagg cccgcccctg
     5941 gcagcagtgg ctggacaacc agcgaatcga tctggccgag ataccacccg gcgacgatcc
     6001 gcacggcttc catcccgcga acctgacgcc gcgcatgcag gcgttcggct acaccgtcga
     6061 gaccatggag atcatgctcc agccgctggt caaggagctg cgagatcccc tggggtccat
     6121 gggcaacgac gccgcgcttg cggtcatgtc cgacaagccg cgaatgatct acgactactt
     6181 caagcagcgc ttcgcccagg tgaccaaccc ggccatcgac tccatccgcg aggaggtgat
     6241 catggcgctg gagtgctaca tcgggccgga gcgcaatctg ctcgaaacca cagaggagca
     6301 cgcccaccgg ctccgggtgc cgcacccgat actttccaac gaggagctgg ccgcgctcaa
     6361 gcacatggac caccgcggct ggcgttcgcg caccatcgac atcaccttca agcaaggcac
     6421 cggcagagac gggctcgtgg ccgcgctcga ccggatctgc gcggaagcca gtggcgccat
     6481 cgccgacggc tacagcctcg tcgttctctc ggaccgcggt atcgggccgg atcgggtccc
     6541 ggtgagctcg ctgctcgccg tcggcgccgt gcaccatcac ctggtccgtg agcagtccag
     6601 gacccgtatc ggcatcgtgc tggaaaccgg agaagcccgg gaggtgcacc accactgcct
     6661 gctggtgggt ttcggcgccg acgccatcaa cccgtatctg gccttcgagg cgctctggca
     6721 agcccgccgc aacggctatc tcgacgacgc cgcgcacatc acctcagacg acgatgtggt
     6781 cgccgcctac cgcaagggca cggcgaaagg catgctcaag gtcatggcga agatggggat
     6841 ctcgaccctg cactcataca agggcgcgca gatattcgag gccgtcggcc tcgccgacga
     6901 cgtcatcgac gtggcctttg tcggcaccgc aagccgtgtc cagggcattg gcctgcgggt
     6961 gctggccgaa gagatggagc gccgccacgt tatcggctat tccaagcggt cccagaacgt
     7021 caccgtgctg cccaacccgg gcgacttcca ctggcggcgc acgggcgata cgcacatgtg
     7081 ggatcccagc gccatcgcca gcctgcaggt tgccgtgcga ggcaacagcg aggacgcgta
     7141 ctggcagttc gccaggcacg tcaacgagga aaccacccgt cacgccaacc tgcgcggcct
     7201 gctcacgttc aacaaggacg tcaacggtgg ccctatcccc atcgaggagg tcgagccggc
     7261 cacggagatc gtcaagcggt tcgccaccgg cgccatgagc ttcggctcga tcagcgccga
     7321 ggcccacgag tcgctggcca tcgccatgaa ccgcctgggg ggcaaatcga acaccggtga
     7381 gggcggcgag gaccccagcc gcttcattcc gctggagaac ggggattcca agcggagcgc
     7441 catcaagcag gtggccagcg gtcggttcgg cgtgacgatc aactatctca ccaacgccga
     7501 cgagctgcag atcaagatca tccagggcgc caagccaggc gagggcggcg agctgcccgg
     7561 ccacaaggtg gacgacatca tcgccaacat ccgccactcg acgcccggcg tcgggctcat
     7621 cagcccgccg cctcatcacg acatctactc catcgaggac atggcccagc tgatccacga
     7681 cctgaagaac gcgaaccccg aggcgcggat cagcgtcaag ctcggcgccg aggtgggcgt
     7741 aggaacggta gcggccggcg taaccaaggc ccgggccgat catctggtca tcgctggcga
     7801 caccggcggc accggcgcct cgccgctgac gtccatcaag cacgcgggcg tgccctggga
     7861 actggggatc gccgagaccc atcagaccct cgtggtcaac aacctccgtt cgcgggtcgt
     7921 tctgcagaca gacggccagc tgaaaacggg ccgcgacgtg
//
LOCUS       DBNHLM010000273         5621 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_40472, whole genome shotgun sequence.
ACCESSION   DBNHLM010000273 DBNHLM010000000
VERSION     DBNHLM010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_40472"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="AC16QF_12665"
     CDS             complement(<1..1193)
                     /locus_tag="AC16QF_12665"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     sulfotransferase family protein"
                     /protein_id="HIR6449459.1"
                     /translation="MQDSVAELRKEAARALNQRAYERAAVLCRRLLQQDPKCADGWFL
                     MGMVAAANGRITKALELVGNAALLDADNAEYLAQKARLHLLLSQPEAARETADRALAA
                     RPEDPLTLDTLGVVYTRTGNYDQARQLLQRAVAAADDNPQFHFNLASVEQFLGNLEAA
                     AAGYERSIELHPRFFRARWALSELRKSDSDARGIDVLEHLLADSQLTAEDELYLAHAL
                     ATEYEKDARHDDAFRVLARAKARRRARIGYAHENDARLFTAIRDAFPVGSLPARAQGD
                     AGSDAIFVIGMPRTGTTLVERILDSHPEVQSLGEIQDFAMAVKTASGSRSGLILDEDV
                     ISRARDADALQIGQSYLQSIAARLGHDGRFVDKMPLNFLYAGFILSTLPGARIVCLKR
                     HPLDT"
     gene            complement(1261..3744)
                     /locus_tag="AC16QF_12670"
     CDS             complement(1261..3744)
                     /locus_tag="AC16QF_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6449460.1"
                     /translation="MSKSALTSALTKVPFARSVMAKSVSLASAGLVAMPVVSVDAATG
                     SSVIEEVIVTSSRREQSVQDIPINITAVSDTMIKELRLDGIAEIARYVPGLTVVDRGP
                     RDEQPDILVRGLNTTGLGPGFISDTVAVYFGEIPLNVDFKPVDMERVEVLIGPQGTLY
                     GQGTLGGAIRYIPKRADTSALSIDARAKAMSNKESDGLSTDIGVTLNIPLVEDVLAAR
                     VNFDRLNDKGFIDYPFVVQESGVSNPEPDFSDPADVAANLREVEDANGEEATSIRANL
                     RFTPTDGIDVNLWYLNQENETEGRQIANRLAIGTGKYESGLRYEEPNDYENELWAIEA
                     TADLRFAEATLIYGETQYDELGQRDQTDLLLNFEYGYEFFPTFSSFTREILEEESDTL
                     EVRLTSQHGGPLSWVVGYFKNEVSSVGTSEEFTPGFDQFAVDNFGGVQLRPDSLEYYS
                     IDDTEEEETAFYGEVSFAITDALDVTVGYRNYEFKADSTSGFALPLLDTVFFGDDPDA
                     INVDLGDPNQGKDDGDLFKFNVSYDIENFGLLYATFSEGYRNGGVNSVPLCTPEQIAS
                     DQQQLCALEDEVLIDPDTIDNYEIGLKGMFLDNTLSLNLAAFFIDWKDLQVAEVTANG
                     NLPITGNGSAAESKGIEMSGRWLINDNWEVMMSYAYTNAELTETAPGLVGPLDALKGT
                     RLPGHAEHQGSFNVVYNRTVWGDVDMTLNYGLVFSTDVFNIPGGDEDTLVDEDGVPGD
                     RGGEAIPGYDVHHLSASFSRDSWTVQAFVDNLWNEYYITGTRTSRRFLQDENLGPGNT
                     INGFTLRSYGQYVGMPRNIGISVSYTFER"
     gene            complement(3923..5107)
                     /locus_tag="AC16QF_12675"
     CDS             complement(3923..5107)
                     /locus_tag="AC16QF_12675"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449461.1"
                     /translation="MAETAAQEPIVTGDELIARARALTPVLAAEAAESERLRRPTDAA
                     IDALRESGIFRMMVPRAYGGYELDLDTFLGVGLALGAGDASLAWVANFYVEHNWIFCQ
                     FPEAFQKPFFAERDYSLAPATVAPTGVAEPVEGGYRLAGRWSWGTGVMHGDWVIVSAV
                     VPGDDPVPDMRFFALPIEEITVDDVWHIEGMLGTGSNDILIDGRVIPEERAVSLNAMR
                     DGRAPGAEIHDGPLYRTPMMPVLVMAAAMPAVGQARAVVDAFKQSLQDRFMPMWGITQ
                     AERPAAQMRVARAELEMRQTEMLMGEVVEDVMARRNEATLADRARWMASLALAVDQSK
                     QIVASVATASGASAHFSSHPLQRAARDLSTLSCHAVFDLDARLETAGKAMLGLDPGPI
                     MI"
     gene            5262..>5621
                     /locus_tag="AC16QF_12680"
     CDS             5262..>5621
                     /locus_tag="AC16QF_12680"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6449462.1"
                     /translation="MSVGIDPELRDALDKQAIHDVCMYYCRAVDRLDAELLERVFHDD
                     ATVEYGVYDGPASGFVANMFPLLESLERTSHCIGNELVAVDGDSAVGEIYVFAYLGER
                     SEAGLTDRILVGRYLDRY"
BASE COUNT          934 a         1791 c         1839 g         1057 t
ORIGIN      
        1 caggtgtcca gcggatgccg tttcagacag acgatccggg cgccggggag ggtgctgaga
       61 atgaagccgg catagagaaa attcaggggc atcttgtcga cgaacctgcc gtcgtgtccc
      121 agtcgggccg cgatggactg cagataactt tgaccgatct gcaaagcgtc cgcgtcgcgg
      181 gctcgcgata tgacatcttc gtccaggatc aggccgctgc gtgagcccga cgcggtcttt
      241 accgccatgg cgaagtcctg aatctcgcca agcgattgaa cctcgggatg actgtccaga
      301 atgcgctcga cgagcgtcgt gcccgttcgc ggcatcccga tcacgaagat ggcatcgctg
      361 cctgcatctc cctgcgcccg tgcgggcagc gaaccgacgg ggaaggcgtc tcttatcgca
      421 gtgaacagcc gcgcatcatt ctcatgggcg tatccgatgc gggcgcgccg cctggctttg
      481 gcccgagcca gcacccggaa cgcgtcgtcg tggcgcgcgt ccttttcgta ctccgtcgca
      541 agggcgtggg ccagatagag ctcgtcctcg gccgtgagct gcgaatcggc gagcaggtgc
      601 tcgagcacgt cgatgccacg ggcatccgaa tccgacttcc tcagctccga cagggcccag
      661 cgcgcccgga agaagcgcgg atggagctca atgctgcgct cgtacccggc ggccgcggct
      721 tccaggttgc cgagaaactg ctccacggac gcaagattga agtggaactg gggattgtcg
      781 tctgcggcgg ccaccgcgcg ctgcagcaac tgccgggctt ggtcgtagtt acccgtgcgc
      841 gtgtagacca cgcccagcgt gtccagggtg agcgggtcct ccggcctggc ggcgagcgct
      901 cggtctgccg tctcccgggc cgcctccggc tgactcagca ggaggtgcag cctcgccttc
      961 tgggccaggt actccgcatt gtcggcgtcc agcagcgcgg cgttgccgac gagctccagg
     1021 gccttggtga tccgcccgtt ggcggccgcc accatgccca tcaggaacca tccgtcggcg
     1081 cactttgggt cctgctgcag aagacgccgg cacagcacgg cggctcgctc gtaggcccgc
     1141 tggttcagcg ctctggcagc ttctttcctg agctcggcaa ctgaatcttg catccgcgca
     1201 ccttaggccg gccgcggcaa cgatacaacg ctgctgctgg ccaggccggt aagggcgggc
     1261 tcaacgctcg aaggtgtagc tcacgctgat cccgatgttg cgcggcatgc ccacgtactg
     1321 gccgtacgag cgcagcgtga agccgttgat cgtgttgccg ggtccgagat tctcgtcctg
     1381 caggaaccgg cgcgaggtgc gggtgcccgt gatgtagtac tcgttccaca gattgtcgac
     1441 aaaggcctga acggtccagg aatcgcggct gaacgatgcc gacaggtgat gcacgtcgta
     1501 acccgggata gcctcgccgc cccggtctcc cggcacgccg tcctcgtcca ccagcgtgtc
     1561 ctcgtcgccg ccgggaatat tgaagacgtc tgtggagaag acgagcccgt agttcagcgt
     1621 catgtccacg tcgccccaca ccgtccggtt gtagacgacg ttgaaggatc cctgatgctc
     1681 ggcgtggccg ggcagacgcg tgcccttcaa cgcgtccaga gggcccacca ggccgggcgc
     1741 cgtctccgtc agctcggcgt tggtgtaggc gtagctcatc atcacttccc agttgtcgtt
     1801 gatgagccag cggcccgaca tctcgatgcc cttgctctca gcggcgctgc cgttaccggt
     1861 gatgggcagg ttgccgttgg cggtgacctc ggcaacctgg aggtccttcc agtcgatgaa
     1921 gaaggcggcc aggttcagcg acagggtgtt gtcgaggaac atgcccttga ggccgatctc
     1981 gtagttgtcg atggtatcgg gatcgatgag aacctcgtcc tcgagcgcgc acagctgctg
     2041 ctgatcgctg gcgatttgtt ccggggtgca caaaggtacg gagttcacgc cgccattgcg
     2101 gtagccctcc gagaacgtgg cgtagagcag cccgaagttc tcaatatcgt aggagacgtt
     2161 gaacttgaag agatcaccgt cgtccttccc ttgattggga tcgcccaggt cgacgttgat
     2221 ggcatccgga tcgtccccaa agaacaccgt gtccagcagc ggcagcgcaa atccgctggt
     2281 gctgtcggcc ttgaactcgt aattgcggta gccgacggtc acgtccagcg catccgtgat
     2341 cgcgaacgag acctcaccgt aaaaggcggt ctcttcttct tccgtgtcgt caatcgagta
     2401 gtactcgagg ctgtccggcc gcagctgcac gccgccgaag ttgtccacgg cgaactggtc
     2461 gaagccaggg gtgaactctt cgctcgttcc gacactcgac acctcgttct tgaagtagcc
     2521 caccacccat gacagggggc cgccatgctg ggaggtgagc cgcacttcca gggtgtcgct
     2581 ttcctcttcc aaaatctccc gggtgaagga agagaaggtg gggaagaact cgtagccgta
     2641 ctcgaaatta agcagcaggt ccgtctgatc gcgctgcccg agctcgtcgt actgggtttc
     2701 cccgtagatg agggttgctt cggcgaatcg caggtcggcg gtcgcttcga tggcccacag
     2761 ctcgttctcg tagtcgttgg gctcctcgta gcgcagtccc gattcgtact tgccggtacc
     2821 gatcgccaga cggttggcga tctgccgtcc ctccgtctcg ttctcctggt tcaggtacca
     2881 gaggttcacg tcgatcccgt cggtgggggt gaatcgcagg ttggcgcgaa tggaggtggc
     2941 ttcctcaccg tttgcgtcct ccacctcacg caggttggct gcgacgtccg ccgggtcgct
     3001 gaagtcgggc tccgggttcg acacgcccga ttcctgaacg acgaacgggt agtcgatgaa
     3061 gcccttgtcg ttgagccggt cgaagttgac gcgtgccgcc agcacgtcct ccaccagcgg
     3121 gatgttcaac gtcacgccga tgtcggtgct caggccgtcg ctctccttgt tggacatcgc
     3181 cttggcgcgg gcgtcgatgc tcagcgcgga ggtatccgcc cgcttgggta tgtagcgaat
     3241 ggcgccgccg agggtgccct ggccgtacag cgtaccctgg ggaccgatca ggacctcgac
     3301 ccgctccatg tcgacgggct tgaagtcgac gttcagcggg atctcgccga agtagacggc
     3361 cacggtgtcg ctgatgaatc cgggccccag ccccgtggtg ttcaggccgc gcaccagaat
     3421 gtccggctgt tcgtcccgcg gcccccggtc gaccaccgtc agcccgggaa catatctggc
     3481 gatctcggca atgccgtcta atcgcagctc cttgatcatc gtgtcgctta ccgcggtgat
     3541 gttgatggga atgtcctgga cgctctgctc ccggcggctg gaggtgacga tcacttcctc
     3601 gatgacggac gagcccgtgg ccgcatcgac cgagacaacc ggcatggcga cgagcccggc
     3661 cgaagccaga gacaccgatt tcgccatgac cgatcgggcg aacggcacct ttgtcagtgc
     3721 agatgtcagt gcagactttg acatgtttcc cccaagttcc ggccgggtgg ccgaggatct
     3781 tgttttttgt tgtggacagc ttgtttttga ccgcgcagcc tatgaacttt gcggcgttcg
     3841 tacaacccga accgcaatga aattgagccg tgagcgccga tccccagggt ccccgccgag
     3901 cccgccgccg gcctcggact tttcagatca tgatcggtcc ggggtcaaga ccgagcatgg
     3961 cctttccggc agtctcgagc cttgcgtcca gatcgaagac cgcgtggcac gaaagcgtgc
     4021 tcagatcccg cgcggcgcgc tgcagcgggt ggctcgagaa atgcgcgctg gcgccgctgg
     4081 ccgtcgcaac ggaggcaacg atctgcttgc tctggtccac cgcgagggcg aggctcgcca
     4141 tccaccgcgc acggtctgcc agggtcgcct cgttgcgccg cgccatgacg tcctcgacca
     4201 cctcgcccat gagcatctcg gtctggcgca tctccagctc cgccctggca acgcgcatct
     4261 gggccgccgg ccgctctgcc tgggtgatgc cccacatggg catgaagcga tcctgaagcg
     4321 actgcttgaa cgcatcgacc accgctctcg cctggcccac ggccggcatg gcggccgcca
     4381 tgacgagcac gggcatcatc ggcgtccggt acagcggtcc gtcgtggatt tcggcacccg
     4441 gcgcacggcc gtcgcgcatg gcgttcaggg ataccgcccg ctcttccggg atcacccggc
     4501 cgtcgatgag aatgtcgttg ctgccggtgc cgagcatgcc ttcgatgtgc cagacgtcgt
     4561 ccacggtgat ctcctcgatg ggcagcgcga agaagcgcat gtcgggcacg ggatcgtcgc
     4621 cgggcaccac ggcgctcacg atgacccagt cgccgtgcat gacgcccgtc ccccacgacc
     4681 agcgtccggc aagccggtag ccgccttcca ccggctcggc gaccccggtg ggcgccacgg
     4741 tggccggggc cagggagtag tcgcgttcgg caaagaaggg tttctgaaag gcctcgggga
     4801 actggcagaa gatccagttg tgctcgacgt agaaattggc gacccacgct aatgaggcgt
     4861 cacccgcacc cagggcgagc ccgacgccca gaaaggtgtc cagatccagc tcgtagccgc
     4921 cgtaggcccg gggaaccatc atccggaaaa tgccggactc ccgcagggcg tcgatggcgg
     4981 cgtcagtggg ccgcctgagc cgttcggatt ctgcggcttc tgccgccagc acgggcgtga
     5041 gtgcccgggc ccgggcgatg agctcatccc cggtcacgat gggttcctgt gcggcggttt
     5101 cggccatggc gcccgctcct tccccaatga ggcgttgtcg tcgatcgaga gtaggacaga
     5161 tgaagcgggg gcacaaaccg gctcgcggct gtgatggcgg gtttcggcgg cggcgggcat
     5221 ggtctaggct agtgctcggg aagaaagggg aggagagaag catgtccgtg gggatcgatc
     5281 cggagctcag agatgctctg gacaagcagg ccattcacga cgtctgcatg tactactgcc
     5341 gggccgtgga tcgcctggac gccgagctgc tcgagcgcgt ctttcacgac gacgccacgg
     5401 tggaatacgg cgtgtacgac gggccggcca gcgggttcgt cgcgaacatg tttccgctgc
     5461 tggagagcct cgagcgcacc tctcactgca tcggcaacga gctggtggcg gtggacgggg
     5521 acagcgccgt gggcgagatc tacgtctttg cctacctcgg cgagcggtcg gaggcgggtt
     5581 tgaccgaccg gattctggtg ggccggtatc tggaccgcta c
//
LOCUS       DBNHLM010000274         3745 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_160253, whole genome shotgun sequence.
ACCESSION   DBNHLM010000274 DBNHLM010000000
VERSION     DBNHLM010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_160253"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="AC16QF_12685"
     CDS             <1..305
                     /locus_tag="AC16QF_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6449463.1"
                     /translation="FDWITSVGFRDSQYMTIFNGEDYQQPEDPRLRLDDKVDSYWTVD
                     MGAAYVHGEGDSWTVEAYVANLTDEENEQAIIITQFDNTRFFQRPRTYGLRFRYRR"
     gene            complement(343..2202)
                     /locus_tag="AC16QF_12690"
     CDS             complement(343..2202)
                     /locus_tag="AC16QF_12690"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6449464.1"
                     /translation="MSNNLIAAAVRPLAGAVMLWSGLGWAQEYSVPVDGDYPKNVYWG
                     DTHLHTRNSPDAYSMGNMNLTPADAYRFAQGQEVVAHNGMRVQLRRPLDFLVVSDHSE
                     YIGGYYRFNVGDPIVTETEAGRQWQGYLDEGGDAVANIYATFVNSMQDPENYPPLPER
                     SRLLIWQDVATTADEYNRPGQFTAFTGYEWTAMIDGNNLHRVVVYKDSADKVSQLPPF
                     SGQDSLDPRELWKALARYEAQTGGEVMAIAHNGNLSNGMMFPDKSVDGKPIDRAYAEL
                     RARWEPIYEVTQVKGDGEAHPTLSPDDEFADYENWDWDNIGRSQAKEDWMLPHEYARG
                     ALKLGLKHEKKLGVNPFKFGLIGSTDGHNTISTTEEDNFFGKFPESEPGPERLENAMA
                     MDRLWKNWNIVASGYAAVWARENTREALFEAMQRREVYATTGSRISVRFFGGWDYGAE
                     DIHRPTYLDVAYAGGVPMGGDLTAAPKDRAPKFMVHAVKDPDGANLDRVQVVKGWLER
                     DGSLQEKVYDVAWSGDRSPDPKTGKLFPVGSTVDVEAATYDNSIGTAQLSTVWEDPDF
                     DAKERAFYYVRVLEIPRPRWTAYDAAFFDLDLPEEIPMVIQDRAYTSPIWYTP"
     gene            complement(2195..3175)
                     /locus_tag="AC16QF_12695"
     CDS             complement(2195..3175)
                     /locus_tag="AC16QF_12695"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6449465.1"
                     /translation="MEDPRLAGIGNSSINLKELRHVLLLHETLSFKRAAELAGVTQSA
                     LSQSIATLEERLGVKLFDRNRRKVSATGFGDLIAGRAVAILNDLSDMDQQIGAMRDVR
                     EGTMRFGMGIVPAVLILEQATSAFQAEHPDVATRVIVDVPEVLAERMEANEIEFFVAH
                     YPPQIRSRDHQHETLLSYEHRLICAPEHPLAGLNRVTFADLIRHPVVSFGPMYLADLG
                     LRTLRDSDDYRRFERNYPAVQTQQPWLLADLVAAGDFVMFAPGEPLADRIGQGLLVTR
                     EVADLQTRIEVKLVWKAGVSRSPAAKRMAEILHELAERQARDDLRRNFVE"
     gene            3386..>3745
                     /locus_tag="AC16QF_12700"
     CDS             3386..>3745
                     /locus_tag="AC16QF_12700"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6449466.1"
                     /translation="MSIKPLHGGPSRLLTLVCLLLPATPGFVVAEERTAAIEEIFVTA
                     RKREEPLQQTPVAVTALTGESLERVFATDITDLHVRAPNVIVSDISGFSSSAAVFIRG
                     IGNADIDSTVDPPVAIFI"
BASE COUNT          698 a         1218 c         1097 g          732 t
ORIGIN      
        1 gcttcgactg gatcacgtcc gtcggctttc gcgactcgca gtacatgacg atcttcaacg
       61 gcgaggacta ccagcagcca gaggatccga gacttcggct ggacgacaag gtagacagct
      121 actggacggt cgacatgggc gctgcttacg tccacggtga gggcgacagc tggacggttg
      181 aagcctacgt ggccaacctg accgacgagg agaacgagca ggccatcatc atcacgcagt
      241 tcgacaacac gcggttcttc cagcgccccc gaacctacgg gttgcgcttc cgctatcgca
      301 ggtgatgctt cggggccgcg gcgcccgccg cggccctccc cttcagggcg tataccagat
      361 cggagaggtg taagcccgat cctgaatgac catggggatc tcttcgggca ggtccaggtc
      421 gaagaaggcg gcgtcgtaag ccgtccaccg gggccgggga atttccagga cccgaacgta
      481 gtagaaggcc cgctccttcg catcgaagtc cgggtcctcc cacaccgtgc tcagctgtgc
      541 cgtgccgatg gagttgtcgt aggtggcggc ctcgacgtcc accgtggagc ccaccgggaa
      601 cagcttgccc gtcttcggat ccggcgaccg gtcacccgac caggcaacat cgtagacctt
      661 ctcctgcaac gatccgtccc tttcgagcca ccccttcacg acctgaaccc ggtcgaggtt
      721 cgcgccgtcc gggtccttca cggcgtgcac catgaacttc ggagcacgat ccttgggcgc
      781 cgccgtcagg tcgccaccca tgggcacgcc gccggcgtag gccacatcca gataagtcgg
      841 gcggtgaatg tcctcggccc cgtagtccca gccgccgaag aagcgcaccg agatccgcga
      901 gccggtggtc gcgtagacct cccgccgctg catggcctcg aacagcgctt cccgggtatt
      961 ttcgcgcgcc cacacggccg cgtagccgga agccacgatg ttccagttct tccagagccg
     1021 atccatggcc atggcgttct ccagccgctc agggccgggc tcggactcag gaaacttgcc
     1081 gaagaagttg tcttcctcgg tcgtggagat cgtgttgtgg ccgtcggtgc tgccgatcag
     1141 gccgaacttg aacggattca cgcccagctt cttctcgtgc ttcagcccga gcttgagggc
     1201 gcctcgggcg tactcgtgcg gcagcatcca gtcctccttg gcctggctgc ggccgatgtt
     1261 gtcccagtcc cagttctcgt agtcggcgaa ctcgtcatcc ggagagaggg ttggatgcgc
     1321 ctctccatcg cccttcacct gggtcacctc gtagatgggc tcccagcggg cgcgcagctc
     1381 ggcgtaggcg cggtcgatgg gtttgccgtc cactgacttg tcaggaaaca tcatgccgtt
     1441 ggacaggttg ccgttgtggg cgatggccat cacctcgcct ccggtctggg cctcgtaacg
     1501 ggcaagcgcc ttccaaagct cccggggatc gaggctgtcc tgccccgaga acgggggcag
     1561 ctgagagacc ttgtccgctg aatccttgta aaccaccacc cggtgcaggt tgttgccgtc
     1621 gatcatcgcc gtccactcgt agccggtgaa ggcggtgaac tgccccggtc ggttgtactc
     1681 gtcggcggtc gtggccacgt cctgccagat cagcaggcgc gagcgctcgg gcaggggcgg
     1741 atagttctcc ggatcctgca tgctgttgac gaaggtcgcg tagatgtttg ccaccgcatc
     1801 gccaccttca tccaggtagc cctgccattg gcggccggct tcggtctcgg tgacgatggg
     1861 atcgcccacg ttgaagcggt aataaccacc gatgtactcg gagtggtccg agaccaccag
     1921 gaaatccagc gggcgccgca gctgcacccg catgccgttg tgcgcaacga cctcctggcc
     1981 ctgggcaaac cgataggcgt ctgccggcgt gaggttcatg ttgcccatgg agtaggcgtc
     2041 cggcgagttg cgcgtgtgca ggtgggtgtc tccccagtac acgttcttgg ggtaatcgcc
     2101 gtccaccgga acggaatact cctgggccca acccaaacca ctccagagca tcacggctcc
     2161 cgccagcggt cgaaccgccg ctgcaataag attattactc aacgaagttc ctcctgagat
     2221 catccctcgc ctgcctctcc gcaagctcgt gtaatatttc cgccattcgc ttggccgccg
     2281 gcgagcgact cacgcccgcc ttccaaacca gcttcacctc gatgcgcgtc tggaggtcgg
     2341 cgacctcgcg tgtgaccaga agaccctggc cgatccgatc ggccaacggc tcaccggggg
     2401 caaacatcac gaagtccccc gccgcgacca ggtctgcaag cagccacggt tgctgagtct
     2461 gcaccgcagg atagttgcgc tcgaaccgcc tgtagtcatc actatcgcgc agcgtccgaa
     2521 ggcccagatc ggcgagatac atcggaccga aactcaccac ggggtggcgg atcaggtcgg
     2581 caaacgtcac ccgattcagg ccggccaacg ggtgttcggg ggcacagatc agacgatgtt
     2641 catagctcaa caacgtctcg tgctggtggt cgcggctgcg aatctgaggg ggatagtggg
     2701 cgacgaaaaa ttctatctcg ttcgcctcca ttcgctctgc cagcacctcg ggcacgtcga
     2761 cgatgacccg tgtcgccacg tccgggtgct cggcctggaa agccgacgtc gcctgttcca
     2821 gtatcaatac ggccggcacg atgcccatcc cgaaacgcat ggtgccttcc cggacatccc
     2881 gcatcgcgcc aatctgctga tccatgtccg acagatcatt caggatcgcc accgcacgcc
     2941 cagcgatcag gtccccaaat ccggtcgctg agacctttct gcgattccgg tcgaacagct
     3001 tgacgcccag gcgctcctcc agcgtcgcga tggactggct cagcgccgat tgggtcacgc
     3061 cggccagctc tgctgcacgt ttaaagctca gagtttcgtg aagcagcagg acgtgccgca
     3121 gctcctttag gttgatggac gagtttccaa tcccggctag gcgaggatct tccatatgac
     3181 tctgctgaag gtgtgtcaaa ctcaatatta gctcaactta tgaaatatat taaaatttat
     3241 tgttttaatt ataactatct gtttttatta atgaattttc ttgaagtcta ctcggaacga
     3301 gttaacatgc ccagccgaaa tgcgccaaag atctcctgct gaactcgttc cgcagtccaa
     3361 catgaaaaac cgaggggatt accacatgtc gatcaaacca cttcacggtg ggccttcgcg
     3421 cctgttgacg ctggtgtgcc tgttgctgcc ggccacaccc ggctttgtcg tcgccgaaga
     3481 gcgcaccgcc gccatcgaag aaatcttcgt caccgcgcga aagcgcgagg agccgctgca
     3541 gcagacaccg gtggcggtaa ccgcgctcac cggggaaagt ctggagcgag tgttcgccac
     3601 ggacatcacc gacctccacg tcagagcacc caacgtgatc gtgtccgaca tcagcgggtt
     3661 ctccagttcg gcggccgtct tcatccgcgg catcggcaac gccgacatcg actcgaccgt
     3721 ggatccgccg gttgccatct tcatc
//
LOCUS       DBNHLM010000275        13685 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1296403, whole genome shotgun sequence.
ACCESSION   DBNHLM010000275 DBNHLM010000000
VERSION     DBNHLM010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13685
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1296403"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..1696
                     /locus_tag="AC16QF_12705"
     CDS             326..1696
                     /locus_tag="AC16QF_12705"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6449467.1"
                     /translation="MTQPRLSKATLFCYGVADLPVMLAIMPMSIWLSRFYTGDMGLSL
                     SAVANIMLLARLFDVFTDPLVGHLSDHTRTRWGRRKPWMLASLPLLMIGLYKVFLPPD
                     GIGIWYLFSWMMVMWLGWTMLMIPYYAWAAELSDDYDERTRITGWRAVMGSVGGIGAQ
                     LIPFIALVLFGFGGTANVMTMLGIAALILMPLCVGVALLRVPEFPEMRAPSVPILAGI
                     RIMWRNGPFRRLLLAFVLSSTGLAIVMPLYIFFVEFIVEEPPANVPYMIIISSVAGFL
                     GIPFWVWLSKHVGKHKAWIGGFLVVAAVSPMYLFLGPGDFWLMVPGIIIIGIGTGSFS
                     ALPNSMKADVIDLDTARSGKNRAAFFFSAWSLVTKLASSLGSWLALQSLALFGFDAAN
                     GAQNTPEALTGLRLTFAVLPAIIFVIAAAVIWRYPITKERQARIRAAIDRRTLRRNAA
                     QAGFAK"
     gene            1704..2909
                     /locus_tag="AC16QF_12710"
     CDS             1704..2909
                     /locus_tag="AC16QF_12710"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HIR6449468.1"
                     /translation="MIARTGVLLVSMLWAASAKAEPVGFAACVSNLESVAVASGISEA
                     TAKQVLGEVRHLERVIELDRTQPEFVRTFSEYYRVRVTPSRVADGRRLLEENAALLGR
                     LQRQYGIPGHYLIALWGLETNFGSYFGNMSTMDSLATLACDERRSAFFTAELMDALRI
                     VDAGDMEPAAMQGSWAGAMGHVQFMPSVFRRYAVDDDGDGRRDLWTSRRDALASAANY
                     LKQAGWERGFRWGREVRLPEAFDYAGAARDPTRPLADWAAAGVRNAFGGPLASTDLPA
                     TLRLPSGHRGPAFLTYPNFEVIMQWNRSESYALSVGNLADRIAGRSPLTRPPPTDELK
                     LTPDSVRQLQADLNALGLDAGEADGVIGPATRAAIGRFQIGHGLVPDGYPDHRLVEAL
                     RASREAGER"
     gene            complement(2939..3850)
                     /locus_tag="AC16QF_12715"
     CDS             complement(2939..3850)
                     /locus_tag="AC16QF_12715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6449469.1"
                     /translation="MKIGLMMGATGTTTLDDVVALAKSAEAAGLDSLWMANIFSFDAI
                     STLAIIGRETSTIGLGTAVTPTYPRHPTAIAQQALTTAAASGSRFTLGIGLSHKIVIE
                     NMLGFSYDRPARHMREYLSVLMPLVRGETCNFQGEQYRVAGVRIDIPGEITMPVVVAA
                     LGPAMLKITGELADGTNTWMVGPKTMAEHIIPRISAAADEAGRPPPRIVGGYPVILTN
                     KPDEAREKIARELTIYGQLPSYRAMLDREGVAGPADIAIAGDENLVRGELKRLEDMGV
                     TDFNAAIMPVEEGAQERTVSFLASLKG"
     gene            3955..4428
                     /locus_tag="AC16QF_12720"
     CDS             3955..4428
                     /locus_tag="AC16QF_12720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6449470.1"
                     /translation="MDVASFTDLSEKFTEITERIVWCTVSTVDAKGRPRSRILHPVWE
                     GATGWIATGRHSHKARHLANNPYVSLSYWDPQHEQTMIECKAEWQDDQPTRDRIWELL
                     KTTPEPVGYDPALFWSSAADENFGVLKLTPWRLEVWSLAAMSAGKPAQVWRQEVG"
     gene            4509..5168
                     /gene="npdG"
                     /locus_tag="AC16QF_12725"
     CDS             4509..5168
                     /gene="npdG"
                     /locus_tag="AC16QF_12725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006740 - NADPH regeneration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR6449471.1"
                     /translation="MAETIAILGGTGDLGTGLAIRWSKAGHKIIIGSRTLEKAQNAVG
                     NLHKISPETPADAMVNADAAAAGDIVVLTVPAEHQLSTLEGVKQSLTGKILIDVTVPL
                     VPPKVGTVQLPPEGSAGKRAQDMLGEDVMVVTAFQNIAAHLLQEDVHIECDVLVAGNK
                     KAARDKVIELVEQAGMKGYHAGPIENSAAAEALTSILIQINRRHDISHSGIKIVGQSD
                     H"
     gene            5179..5973
                     /gene="cofE"
                     /locus_tag="AC16QF_12730"
     CDS             5179..5973
                     /gene="cofE"
                     /locus_tag="AC16QF_12730"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HIR6449472.1"
                     /translation="MDKPEATSRTASPGFSVLGIPGVPMVSPGDDLTAIIVDALRDAR
                     ITLEDGDIVCVAQKIVSKAEGRQIPLASVTPSEEAQTLAEETDKDPRLVELILRESSE
                     VMRKKEGVLIVRHRLGLVGANAGIDQSNISHEDGEQALLLPEDPDRSAAELKSALDEA
                     SGARVGVIITDSANRPWRLGTVGIAIGCAGISVLDDHRGGVDLFDRELKVTLISRADG
                     IAGAATLVMGETTERIPVALVRGFPAEQSTDDARMVCRPLHEDLFR"
     gene            5973..6902
                     /gene="cofD"
                     /locus_tag="AC16QF_12735"
     CDS             5973..6902
                     /gene="cofD"
                     /locus_tag="AC16QF_12735"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HIR6449473.1"
                     /translation="MSGYLAITGGVGGAKLGLGLAQILPPDELTFAVNTGDDFEHLGL
                     HIAPDVDTLTYTLAGLSNTETGWGRADETWDFMDALDGLGGETWFRLGDRDLALHVMR
                     THLLSAGACLTEVTDHICRALGVAHRVLPMSDDPVRTTVHTERGPLPFQHYFVRERCE
                     PAVTGFEFAGIGQARLNPAITSRLDGLRGIIICPSNPFVSIDPMLNLRGARAALKESG
                     VPIVAVSPIVGGAAIKGPTAKMMRELNVPATAAQVAEHYGDLLCGLVLDEQDAHLEGT
                     LSTPTIVAQTVMVTLQDRVDLGRTVMDFIEQLS"
     gene            6941..7612
                     /gene="cofC"
                     /locus_tag="AC16QF_12740"
     CDS             6941..7612
                     /gene="cofC"
                     /locus_tag="AC16QF_12740"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HIR6449474.1"
                     /translation="MWAVVPLKQLEQAKARLANVLTAEERRALMLAMARDVLTALGRS
                     KRLTGILIVSRTPEADALAQAFGTERFAEGPGANLSQALVQASEHLASRLEARGVMIV
                     PADVPLITPAEIDAILEQQDRVTVVPDDENVGTNCLICSPPNCIPYVFDGSSFKPHVD
                     AAFSLGITPTIMPSAGFALDIDTPDDLRTLLDRDPACQTGSYLAKSGIAERLSDADNG
                     SLTSR"
     gene            7616..10093
                     /gene="cofH"
                     /locus_tag="AC16QF_12745"
     CDS             7616..10093
                     /gene="cofH"
                     /locus_tag="AC16QF_12745"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: protein motif:HMM:NF005609.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HIR6449475.1"
                     /translation="MALKTLIDDIRALALAGGLPSPAQALALADEPDTAALAAIAAEV
                     RDRGFQNVVTYSRKLFIPLTHLCRDVCHYCTFAQVPRKLKAPYMSVDEVLEIARHGAA
                     MGCKEALFTLGEKPELRYRAAREALAELGFETTLDYLAHVAEIVYRETGLLPHLNPGT
                     MTPEELARLRPLSPSMGIMLESTAERLGEKGMPHHGSPDKVPAVRLETLDHAGAARVP
                     FTTGILIGIGETRRERIESLLAIREVHERHGHVQEIIVQNFRAKPETKMARAPEPDLN
                     ELLWTIAVTRILFGSAMSVQAPPNLSPGVLAQIVAAGINDWGGVSPLTPDFVNPEAPW
                     PHLDDLARETAAAGKDLFERLTVYPGYVRDLDTWVAPELQERVLELSDSEGFARTSQW
                     CPGAEVEPPARLLESVTRPPERVSADLERILRKAGDGRRLEASEVVRLFQARGEDFSA
                     VVQAADGLRRETNGDVVSFCVNRNINYTNICYFKCQFCAFSKGKLSENLRGRPYDLSH
                     EEIARRTQEAWSRGATEVCMQGGIHPEYTGETYVGILQAVKEAVPEMHIHAFSPLEVW
                     QGAATLGLDLDTYLERLKAAGLGTLPGTAAEILDDEIRQIICPDKINTEQWLDVMRAA
                     HRVGFKTTATIMYGHVESAVNWARHLLRIRDLQAETGGFTEFVPLPFVHMEAPMYLKG
                     KARKGPTFREAILMHAVARLTLHPLIPNIQASWVKMGHAGVQACLQAGCNDLGGTLMN
                     ESISRAAGTTHGQETSPQRMAEMIKAVGRIPRQRTTLYEDASEERIAVGLAAGELTEI
                     VNTPARKYERKRSGPLLRPGLIDAVEV"
     gene            10138..11193
                     /locus_tag="AC16QF_12750"
     CDS             10138..11193
                     /locus_tag="AC16QF_12750"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR6449476.1"
                     /translation="MAVPWPEAPLFAADVPELQDFLIVEVETASGMVGMGYLQPLAGG
                     LKTLACCVHEMLAPLLVGQEADDVEALWDRMWSATYIQGRMGITVMAMSALDIALWDL
                     VGKRAGEPLYRLWGGVDQHIPTYGSGCYRGLGQDGMIRKAERYAADGFSAIKMQVAHM
                     ATHDQDVENVAAMRAALGADIEIMVDVNQGWTAAEAIEVGSRLDESGIYWLEEPVIAH
                     DFAGYRQVARALKTRVVGGENHFTHHDLQPFFDDPCVPILQPDVMRGGLTELKRIAAM
                     AEPLGISIAPHLFPELMTHLLASIPNGSWLEYMGWHDELWAQPVVPSGGLTRPPDRPG
                     HGLAFRADVLAEYRVAG"
     gene            complement(11190..12416)
                     /locus_tag="AC16QF_12755"
     CDS             complement(11190..12416)
                     /locus_tag="AC16QF_12755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6449477.1"
                     /translation="MHDDDKTNDDLGLGRNPTVTLLLAFFQVFLVIMPVAVPFFQSKG
                     LSMQEVFSLQALFALVVLLAEVPSGYVADIFGRKRTLIVGAAFCGLGHTLLLGADGFW
                     TLALFEISLGIGSSLISGADIALLYDTEVALGRGERQQRQVVGRLYSMRTFSEAVAGV
                     VCSVVLLWSMDLAVYVQALIGWLPLVFAFALVEPPGERMERSGHLVNMARICRYLVSH
                     SAVLRLTFLALCIWALTTFYAVWLLQKIWEAQGLGLIHFGYLWGFLSLVAALAGRWAY
                     RAEEMLGTTGVLVFIGLAPAAGYLCLDLFGVVGSVLAGLTFWISRGLGMVILRDALNR
                     RIPGEFRATANSLASFGFRGAFVLTGPLVGYAFDLWGMSVTLSLLALATLVIFAGLVL
                     PLVAAARSQRLEAQAT"
     gene            complement(12846..>13685)
                     /locus_tag="AC16QF_12760"
     CDS             complement(12846..>13685)
                     /locus_tag="AC16QF_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449478.1"
                     /translation="ILASMSQAQFVGTLALFSIMPPYFLCMVTLQRLRTEESLAEMAD
                     LLEPMSAARVQGCIAGIHPAGWFLVVLGAVFGGYQNEEFIRRVGASGTLNAMDVAFVL
                     ANCFVWAAIAFVLSWRLPISLALSRLGAQMKVDVYRLDRLRPLARIATLDILVVAGAM
                     ALMPLQSLDAEFRIYNYNYGIIVGVPSAIVLFVLPLWGVRGNIGDRKADRVAVLQAKL
                     DVVDRNDVAAVEALSAHIERLRGLSNWPVDLRLATRIFAYVIIPPLAWVGAALVEILI
                     DRL"
BASE COUNT         2466 a         4625 c         4423 g         2171 t
ORIGIN      
        1 agacaccgat tactatgcct ctaaggaggg catggcggcc gaggaacggt tccagaagac
       61 cagcacctgc accaactcca tctggggcgg ctactggatc gtaccgcccg ccgagggccc
      121 caccgcccaa tagggtactc caccatgccg gccctggaag cacaccaggg tcggcatgac
      181 gccgtcgccg gcttgggaac cgtcacgacg ggattgacga ccagcggccg atcgccgagc
      241 acccagagcg cgccgagtca tggtggattc ctcaggcgcg gccgacttac actgaagcct
      301 ccaagcccgc caatcgggaa cgcagttgac ccagccgcgg ctcagcaagg ccaccctgtt
      361 ctgctacggc gtcgccgatc ttccggtgat gctggcgatc atgcccatgt ccatctggct
      421 gtcgcgcttc tacaccggcg acatgggcct gagcctgtcg gcggtggcca acatcatgct
      481 gctggccagg ctcttcgacg tgttcaccga ccccctggtg gggcacttga gcgatcacac
      541 ccgcacccga tggggcaggc gcaagccctg gatgctggcg tcgctgccat tgctcatgat
      601 cggcctgtac aaggtgtttc tgccgcccga cggcatcggc atctggtacc tgttctcgtg
      661 gatgatggtc atgtggctcg gctggaccat gctgatgatt ccctactacg cctgggccgc
      721 cgagctctcc gacgactacg acgagcgcac gcgcatcacc ggctggcgcg cggtcatggg
      781 cagcgtggga ggcatcggcg cccagctgat ccccttcatc gcgctggttc tgttcggctt
      841 cggcggcacc gccaacgtca tgaccatgct aggtatcgcg gcgctcattc tcatgcccct
      901 gtgcgtgggc gtggcgctgc ttcgggtgcc cgagtttccg gagatgcggg cgccgtcggt
      961 gcccatcttg gcgggcatcc ggatcatgtg gcgcaacggt ccgttccgtc gcctgttact
     1021 cgctttcgtc ctgtcctcca ccgggctcgc catcgtcatg ccgctgtaca tcttcttcgt
     1081 cgagttcatc gtcgaggagc cgcctgccaa cgtcccctac atgatcatca tcagctcggt
     1141 agccggtttt ctgggcatac cgttctgggt ctggctctcc aagcacgtag ggaagcacaa
     1201 agcgtggatt ggcggctttc tggtggtagc cgcggtgagc cccatgtatc tcttcctcgg
     1261 ccccggcgac ttctggctga tggtgcccgg catcatcatc atcggtatcg gcaccgggtc
     1321 cttcagcgcc ctgcccaact ccatgaaggc cgacgtgatc gacctggaca cggcacgcag
     1381 cggcaagaac cgggccgcct tcttcttctc cgcgtggtcc ctggtgacca agctggcctc
     1441 gagccttggc agctggctgg ctctgcagtc tctggccctg ttcggcttcg acgcggccaa
     1501 cggtgcgcag aacacgccgg aggcgctgac cgggctgcgg ctcaccttcg ccgtgctgcc
     1561 ggccatcatc ttcgtgatag ctgccgccgt gatctggcgc tacccgatca ccaaggagcg
     1621 ccaggcccgc atccgggcag ccatcgaccg gcgcacgctg cgccgaaacg cagcccaagc
     1681 aggcttcgcg aagtaacgcc gcggtgatcg cgcgaacggg cgtgctgctc gtatccatgc
     1741 tctgggccgc gtcggctaaa gccgagcccg tggggttcgc ggcctgcgtc agcaacctgg
     1801 aatccgttgc cgtggcgtcc gggatttccg aggcgaccgc gaagcaggta ctcggcgagg
     1861 tacgccacct ggagcgggtg atcgagctcg accgcacgca gccggagttc gttcgcacgt
     1921 tcagcgagta ctaccgggtg cgggtgaccc catcgagggt ggccgacggg cgccgcctgc
     1981 tcgaggagaa tgccgccctt ctcggccggc tgcagcgcca gtacgggatt cccggccact
     2041 acctgatcgc gctgtggggt ctggagacca acttcggcag ctacttcggc aacatgagca
     2101 ccatggactc gctcgccacg ctggcctgcg acgagcgccg cagcgccttc ttcacggcgg
     2161 agctgatgga cgccctgcgc atcgtcgacg ccggggacat ggagcccgca gccatgcagg
     2221 gctcctgggc gggcgccatg ggccacgttc agttcatgcc ttcggtgttc cgccgctacg
     2281 ccgtggacga cgacggcgac ggcagaaggg atctctggac cagccggcgc gacgccctgg
     2341 catctgcggc caattacctc aaacaggctg gctgggagcg gggtttccgc tgggggcgcg
     2401 aggtacgcct gcccgaggcg ttcgactacg cgggcgccgc ccgtgacccc acgcggcccc
     2461 tcgcggactg ggcggccgcc ggggtgcgca acgcgttcgg aggcccgctg gcgtccacgg
     2521 atctgccggc caccctgcgc ctgccctccg ggcaccgggg gcctgcgttt ctgacgtatc
     2581 ccaacttcga ggtgatcatg cagtggaatc ggtcggagag ctacgcgctg agcgtcggca
     2641 acctggccga ccggatcgcc ggacggtcac cgctcaccag gccgccaccc acggacgagc
     2701 tgaagctgac gccggactcg gtgcgccagc tgcaggcgga cctgaacgcc ctggggttag
     2761 atgccggcga agccgacggc gtgatcggcc cggcgacccg ggccgccatc gggcggttcc
     2821 agatcggtca cggtctggtc ccggacgggt atcccgacca tcggctcgtg gaggccctgc
     2881 gcgccagccg ggaagccggc gagcgctgaa cctcaaaagg gatcgctgaa gccgggactc
     2941 agcccttgag gctggccagg aacgacaccg tgcgctcctg cgccccttcc tccaccggca
     3001 tgatcgcggc gttgaaatcc gtcaccccca tgtcctcgag ccgcttgagc tcgccgcgca
     3061 ccaggttttc gtctccggca atggcgatgt ccgcggggcc cgccaccccc tcccggtcga
     3121 gcatggcgcg gtagctggga agctggccgt agatggtgag ttcccgggcg atcttctccc
     3181 gtgcttcgtc gggcttgttg gtcaggatca ccgggtaacc gcccacgatg cgcggtggcg
     3241 gacgcccggc ctcgtccgca gcggcgctga tccgggggat gatgtgctcg gccatggtct
     3301 tcgggcccac catccaggtg ttggtgccgt cggccagctc accggtaatc ttgagcatcg
     3361 ccggcccgag agcggcgacc accacgggca tcgtgatctc gccggggatg tcgatccgca
     3421 cgccggcaac ccggtactgc tcgccctgaa aattgcaggt ctccccccga acgagcggca
     3481 tcagcacgct gagatattca cgcatgtgtc gcgcgggtcg gtcgtaggag aagccgagca
     3541 tgttctcgat cacgatcttg tgagaaagcc cgatacccag ggtaaaccgg ctgccgctgg
     3601 cggccgccgt ggtcagggcc tgctgggcga tggccgtggg gtgcctcggg taggtgggcg
     3661 taaccgcggt gccaagacct atggtgctgg tctcgcggcc gataatggcc agggtggata
     3721 tggcatcgaa ggagaagatg ttcgccatcc agaggctgtc gagcccggcc gcttcagcgc
     3781 tcttcgccaa agcgacgacg tcgtcgagcg tggtggtgcc ggtggcgccc atcatgagtc
     3841 ctattttcat ttctgccccc taggcgatgg ttgtacaggt cgggaaatgc cgcacaataa
     3901 ggcaaaccag ggccggcaac aaagccgcct ggaggcgaac ggggaggccg gcgagtggac
     3961 gtggcgtcat ttacggatct cagcgagaag ttcacagaga tcacggagcg catcgtgtgg
     4021 tgcacggtca gcacggtgga cgccaagggc cggccccgct cgcgcatcct ccatccggtc
     4081 tgggagggcg ccacgggctg gattgccacg ggccgccact cgcacaaggc ccggcacctc
     4141 gcgaacaacc cctacgtctc gctgagctac tgggatcctc agcacgagca gacgatgatc
     4201 gaatgcaaag ccgagtggca ggacgaccag cccacccggg accggatctg ggaactgctg
     4261 aagaccacgc cggagcccgt gggctacgac cccgcgctgt tctggtcaag cgctgcggac
     4321 gagaatttcg gtgtcctcaa gctcacaccc tggcggctcg aggtctggtc cctggccgcc
     4381 atgtccgccg gcaagccggc gcaggtatgg cgccaggaag tcggttagcg gcataatcgc
     4441 ccgcttttca ccgccgtcag cgcctgcacc cgggcgcgcc ggcaatgagc aacaagcggg
     4501 agaaagacat ggcagagacc atagccatac ttggcggtac cggagatctc ggcaccgggc
     4561 tggccatacg gtggtccaaa gcaggccaca agatcatcat cggctcgcgg acgctggaga
     4621 aggcgcagaa cgcggtgggc aacctccaca agatcagccc ggaaacgcca gccgacgcca
     4681 tggtgaacgc cgatgccgcc gcggctgggg acatcgtcgt cctgaccgtt cctgccgagc
     4741 atcagctgtc gacgctggag ggcgtcaagc aatcgctcac cgggaagata ctcatcgacg
     4801 tgaccgtgcc cctggttccc ccgaaggtcg gcaccgtgca gcttccgccc gagggcagcg
     4861 ccggcaagcg ggcccaggac atgctgggtg aggacgtgat ggtcgttacc gcctttcaga
     4921 acatcgccgc ccacctcctt caggaagacg tgcacatcga gtgcgacgtg ctggtggcgg
     4981 ggaacaagaa ggccgcccgg gacaaggtca tcgagctggt cgagcaggcc ggcatgaagg
     5041 gctaccatgc ggggcccatc gagaattccg ccgcggccga agcgctcacc tcgatcctga
     5101 ttcagatcaa caggcgccac gacatctccc actccgggat caagatcgtc ggtcaatcgg
     5161 atcactgacc aaacgcgaat ggacaagccg gaagccacca gcaggacggc atcgcccggc
     5221 ttttccgtgc tgggcatccc gggcgtgccc atggtgagcc ccggggacga cctgacggcc
     5281 atcatcgtcg acgccctgcg cgatgcccgc atcaccctgg aagacggcga catcgtctgc
     5341 gttgcgcaga agatcgtctc caaggccgag ggacgccaga tacctctggc gtcggtgacg
     5401 ccgagcgagg aggcccagac gctggcggag gaaacggaca aggacccgcg gctcgtcgag
     5461 ctgatcctgc gcgaatcgtc agaggtgatg cggaaaaaag agggcgtgct gatcgtgcgc
     5521 catcgccttg gcctggtcgg agcaaacgcg gggatcgacc agtcgaacat ctcccacgag
     5581 gatggcgagc aggcgctcct ccttcccgag gacccggacc gctcggcggc cgagctgaag
     5641 tctgccctcg acgaggccag cggcgcccgg gtcggcgtga tcatcaccga cagcgccaat
     5701 cgcccctggc ggctgggcac cgtgggcata gccatcggct gcgcgggcat cagcgtgctc
     5761 gacgatcatc gcggcggcgt cgatctgttc gaccgggagc tgaaggtcac cctcatcagc
     5821 cgggcggacg gcatcgccgg cgccgccacc ctggtcatgg gcgaaaccac ggagagaatt
     5881 cccgtggccc tggtgcgcgg cttcccggcc gagcaatcca ccgacgacgc ccgcatggtc
     5941 tgccgacctc tgcacgagga tctgtttcga taatgtccgg gtatctcgcc atcacgggtg
     6001 gcgtgggcgg ggcgaaactg gggctcggtc tcgcgcagat cctgccgccg gacgagctca
     6061 ccttcgccgt gaatacgggc gatgacttcg agcacctggg tctgcacatc gcgccggacg
     6121 tcgacaccct cacctatacc ctggcggggc tgagcaacac cgagaccggc tgggggcgcg
     6181 ccgacgagac ctgggatttc atggatgccc tggacggcct cggcggtgag acctggttcc
     6241 gtctcggcga tcgggacctg gcgctgcacg tgatgcgaac ccacctgctg tcggccggtg
     6301 cttgcctcac cgaagtcacc gaccacatct gccgagccct gggcgtcgcc caccgggtgc
     6361 tgccgatgtc cgacgatcct gtgcgcacga ccgtgcacac tgaacggggg cccctgcctt
     6421 tccagcatta cttcgtgcgg gaacggtgcg agccggcggt gaccgggttc gagttcgccg
     6481 ggatcgggca ggcgcggctc aacccggcga tcacgtcgcg cctggacggc cttcgcggca
     6541 tcatcatctg cccctccaac ccgttcgtct ccatcgaccc catgctgaac ctgcgcggcg
     6601 cccgcgccgc tctaaaagaa agcggcgtgc ccatcgtggc ggtctctccc atcgttggcg
     6661 gagccgccat caaggggccc accgcgaaga tgatgcgcga gctcaacgtc ccggcgaccg
     6721 cggctcaggt ggccgagcac tacggcgatc tgctctgtgg actcgtgctc gacgagcagg
     6781 atgcgcatct ggaaggcacg ctgagtaccc cgactatcgt ggcccagacc gtcatggtaa
     6841 cgttgcagga ccgtgtggat ctcggccgca cggtcatgga tttcatagaa caactcagct
     6901 gattcaagct gaaaccaagc aagacggaga gaacctcccc atgtgggccg tggtaccgtt
     6961 gaaacagctc gaacaggcga aggcccggct ggccaacgtt ctgacggcag aggagcgccg
     7021 ggcgctcatg ctcgccatgg cgcgcgacgt gctcacggcc ctcggccgct ccaagcggct
     7081 gacgggcatc ctcatcgtat cccgtacacc agaagcggac gccctggccc aggccttcgg
     7141 cacggagcgc ttcgcggaag gccccggcgc gaatctttcc caggccctcg tccaggcgag
     7201 cgagcacctc gcctcgcggt tggaagcccg cggcgtgatg atcgtgcccg ccgacgtgcc
     7261 gctgatcacg ccggcggaga tcgacgcgat cctcgagcag caagatcggg tcaccgtggt
     7321 cccggacgac gagaacgtcg gcacgaactg cctgatctgc tcaccaccca actgcatccc
     7381 ctacgtcttc gacggttcca gtttcaagcc ccatgtggac gctgcgttct ccctgggcat
     7441 cacgcccacc atcatgccct ccgccggctt cgccctggac atcgataccc ccgacgacct
     7501 gcgcactttg ctggaccgcg atccggcctg ccaaacgggc tcgtatcttg ccaaatcagg
     7561 tatagccgag cggcttagcg atgccgataa tggctcgctg acctcgaggt aggaaatggc
     7621 cctgaagacg ctcatcgacg acattcgcgc cctggccctg gccggcgggc ttcccagccc
     7681 ggcacaggcc ctcgcgctgg ctgacgagcc ggacaccgcc gccctggcgg cgatcgccgc
     7741 tgaagtacgc gaccgcggct tccagaacgt ggtgacctat tccagaaagc tattcattcc
     7801 gctcacccat ctgtgtcggg acgtctgcca ctactgcacg ttcgcccagg tcccgcggaa
     7861 gctcaaagcg ccctacatga gcgtggacga ggtgctcgaa atcgcccgtc acggcgccgc
     7921 catgggttgc aaggaagccc tgttcaccct aggcgagaaa cccgagctgc gttaccgagc
     7981 agcccgggaa gcgcttgccg aactgggttt cgagaccacg ctcgactatc tggcccacgt
     8041 ggcggagatc gtctatcggg agaccggcct gctgccgcac ctcaaccccg gcaccatgac
     8101 ccccgaggag ctcgcccggc tgcgccccct ctcgccatcc atgggcatca tgctggaatc
     8161 cacagccgag cggctgggcg agaaagggat gcctcaccac ggctcgccgg acaaggtgcc
     8221 cgccgtgcgt ctcgagaccc tcgaccacgc cggcgccgcg cgggttccgt tcaccaccgg
     8281 cattctcatc ggtatcggcg agacccgccg ggagcgcatc gaatcgcttc tggccatccg
     8341 cgaggtgcac gagcgccacg gccacgtgca ggagatcatc gtccagaact tccgggccaa
     8401 gcccgagacc aagatggccc gcgcacccga gcccgacctc aacgagctgc tctggaccat
     8461 cgcggtgacc cgcatcctgt tcgggtccgc catgagcgtg caggcgcccc cgaacttgag
     8521 cccgggggtg ctcgcacaga tcgtggcggc cggcatcaac gactggggcg gcgtctcgcc
     8581 gctgacgccg gatttcgtca acccggaagc gccgtggccg cacctggacg atctcgcaag
     8641 agagacggcg gcggccggca aagacctttt cgaacgcctg acggtctatc cgggctacgt
     8701 ccgggatctc gacacatggg ttgcacccga gctgcaggag cgcgtgctgg agctctccga
     8761 cagcgaaggc tttgcccgca ccagccagtg gtgtccgggt gccgaggtgg agccaccggc
     8821 gaggctgctc gagtccgtaa cgcggccacc cgagcgcgtc tcggcggatc tggaacgtat
     8881 tctccgcaaa gcgggcgatg gccggcggct ggaggcgtcc gaggtggtgc gtctgtttca
     8941 agcgcggggc gaggacttca gcgccgtggt tcaggcagcc gacgggctgc gccgggagac
     9001 gaacggcgac gtggtgagct tctgcgtcaa ccgcaacatc aactacacca acatctgcta
     9061 cttcaagtgc cagttctgcg cgttctccaa gggcaagctg tcggagaatc ttcgcgggcg
     9121 tccctacgac ctgtcccacg aggagattgc ccgccgtacc caggaagcat ggtcccgggg
     9181 cgctacggaa gtgtgcatgc agggcggcat acaccccgaa tacacgggtg agacttatgt
     9241 gggcatcctc caagcggtca aggaagccgt ccctgagatg cacatccacg cgttttcccc
     9301 gctggaggtc tggcagggcg cagcaacgct ggggctggat ctggatacgt acctcgaacg
     9361 cctcaaggcg gccggcctgg gtaccctgcc cggaacggcg gcggagatcc tcgacgatga
     9421 gatccggcag atcatctgcc cggacaagat caacaccgag cagtggctgg acgtcatgcg
     9481 cgcggcccat cgggtagggt tcaagaccac ggcgaccatc atgtacgggc acgtggagag
     9541 tgccgtcaac tgggcccggc acctgctgcg gatccgcgat ctgcaggcgg aaaccggcgg
     9601 tttcacggag ttcgtgccgc tcccgttcgt ccacatggaa gcgcccatgt acttgaaggg
     9661 caaagcccga aagggtccaa ccttccgcga ggcgatcctg atgcacgccg tggcgcgcct
     9721 gaccctgcac ccgctgatcc cgaacattca ggcgagctgg gtgaagatgg gccacgcggg
     9781 cgtgcaggcg tgcctgcagg ccggatgcaa cgatctgggc ggcaccctga tgaacgagag
     9841 catcagccgg gcggcaggga ccacccacgg tcaggaaacg tcaccgcagc gaatggcgga
     9901 gatgatcaag gcggtggggc gaatcccgcg gcagcgaacg accctttacg aggacgccag
     9961 cgaagagcgc atcgcggtgg gccttgcggc gggcgagctc accgagatcg tcaacacgcc
    10021 ggcgcggaag tacgagcgca agcgatcggg ccccctcctc cgtccgggtc tcatcgacgc
    10081 ggtcgaggtc taatcctgac ccaaccagat cccatcgcgg acatccggac cacgcggctg
    10141 gccgtgccct ggccggaggc acctctgttc gcagccgacg tcccggaact ccaggacttt
    10201 ctgatcgtcg aggtcgagac cgcgtccggc atggtgggaa tgggctacct gcagccgctg
    10261 gcgggcgggc tgaaaaccct ggcctgctgc gttcacgaga tgcttgcacc cctgctggtc
    10321 ggccaggagg ccgacgacgt cgaagcgctg tgggaccgca tgtggagcgc cacctacatc
    10381 caggggcgga tgggcatcac cgtgatggcc atgtcggcgc tggacatcgc tctgtgggac
    10441 cttgtcggca agcgcgcggg cgagcccctg tacagactat ggggcggggt cgaccagcac
    10501 atcccgactt acgggagcgg ttgctaccgc ggcctcggcc aggacgggat gatccgcaag
    10561 gccgagcgtt acgcagccga cggcttttcc gccatcaaga tgcaggtggc ccacatggcc
    10621 acgcacgatc aggacgtgga gaacgtggcc gccatgcgcg cagcgcttgg ggcggacatt
    10681 gaaatcatgg tggacgtcaa ccagggctgg acggcggcgg aagccatcga ggtcggatcg
    10741 cggctggacg aatcgggcat ctactggctt gaagagccgg tgatcgccca cgattttgcc
    10801 ggctaccggc aggtcgcccg ggccctgaag acacgggtgg tcggtggcga gaaccacttc
    10861 acccaccacg atcttcagcc cttcttcgac gatccctgcg tgcccatcct gcagcccgac
    10921 gtcatgcgcg gcgggctgac ggagctcaag cggatcgcgg ccatggcaga gcccctcggc
    10981 atcagcatcg cgccccacct gttccccgag ctcatgaccc accttctcgc cagcattccc
    11041 aacgggagct ggctcgagta catgggctgg cacgacgagc tgtgggcgca gccggtggtc
    11101 ccgtccggcg ggcttacgcg cccgccggac aggcccggac acgggctggc gttcagagcc
    11161 gacgtcctcg ccgagtaccg cgtcgccggc taggtagcct gggcttccag cctctggctg
    11221 cgggcggcgg caaccagcgg cagcaccagc ccggcgaata tgaccagcgt cgccagcgcg
    11281 agcaggctca gcgtgacgct catgccccag aggtcgaagg cgtagcccac caggggcccg
    11341 gtcaggacga aggcgccccg gaagccgaag ctcgccagcg agttggccgt cgcccgaaac
    11401 tccccgggaa tccgccgatt gagcgcatcc cgcaggatca ccatcccgag cccccgactg
    11461 atccagaacg tcagcccggc aagaacgctg cccaccactc cgaacaggtc taggcaaagg
    11521 tacccggcgg cgggcgccag gccgatgaag accagcacgc ccgtggtgcc gagcatctcc
    11581 tcggcccggt aagcccaccg tccggccaga gcggcgacca ggctcaggaa cccccagagg
    11641 taaccgaagt gaatcagtcc caggccctgt gcctcccaga tcttctgcag cagccagacg
    11701 gcgtagaacg tcgtcagcgc ccagatacac agcgccagga aggtcagccg gagcacggcg
    11761 ctgtggctca ccagatagcg acagatacgg gccatgttca ccaggtgccc cgagcgctcc
    11821 atacgctcgc ccgggggctc gaccagagcg aaggcgaaca ccaggggtag ccagccgatc
    11881 agcgcctgaa cgtaaacggc caggtccatg gaccacagca gcacaacgct gcagacgacg
    11941 ccggccactg cttccgagaa cgtccgcatc gagtacagcc gaccgaccac ctgccgttgc
    12001 tgacgctcgc cgcgccccag cgccacttcc gtgtcgtaga gcaacgcgat gtcggcgccc
    12061 gagatcaggc tgctgccgat gcccagcgat atctcgaaca gcgccagcgt ccagaacccg
    12121 tccgcgccga ggagcagcgt gtggcccaga ccgcagaacg ccgcgccgac gatgagcgtg
    12181 cgcttgcgac cgaagatgtc ggcgacgtag ccggaaggga cctcggccag gagcaccacc
    12241 agggcaaaca gagcctgcag cgagaacacc tcctgcatgc tcagcccctt gctctgaaag
    12301 aacggcacag ccaccggcat gatgaccaga aacacctgga agaatgccag cagcagcgtc
    12361 accgtcgggt tgcgacccaa cccgagatcg tcgttcgtct tgtcatcgtc gtgcatcttc
    12421 atccctccga ccgttcgccg ggacccgcca cggacgccgg aatccggcgc ccagaaaaga
    12481 aaaaccccga aggcttgcgg ctcttcgggg tttttttcgg aactcgctgc tgcgtttcct
    12541 aaagcatcat ccgctcgagc cgtgccctcc tcgcgcaata cgcaccatgt ggtccgcaca
    12601 cagggactgc gcagacacac ggccacccgc gaaggtggta gctggtttgt ttcgatacgc
    12661 gttcatgatc tcgagcttct ctgatccggc tcacgccgaa aaaacagggc gcgcatgttg
    12721 cgccgggcgt tctctggtgt caacggaagt cagcaaaaaa agggggaatc tgtgacccct
    12781 ttcagcaagc cagcctccag acagcttcga gcatcggggt ccgatcgctg gggttgggct
    12841 tgctgctaga ggcgatcgat cagaatctcg accagcgccg cgcccaccca tgccaacggc
    12901 gggatgatca cgtacgcgaa gatgcgggtg gcaaggcgca gatcgaccgg ccagttggaa
    12961 agaccccgca aacgttcaat gtgtgcgctc agggcttcta cggcggctac gtcattgcgg
    13021 tccaccacat ccagcttcgc ctgcagcacg gcaacccgat cggccttgcg gtcgccgatg
    13081 ttcccgcgca ccccccacag gggcagaacg aacagcacga tggcagacgg cacacccacg
    13141 atgatcccgt agttgtagtt atatatccgg aactcggcat ccagggactg cagcggcatc
    13201 aacgccatgg cgccggcaac caccaggatg tccagcgtgg cgattcgggc cagcggccgc
    13261 agccgatcca gccggtagac gtcaaccttc atctgcgcgc ccagtcgcga cagcgccagg
    13321 ctgatcggca ggcgccagct gagaacgaac gcgatggcgg cccagacgaa acagttggca
    13381 agcacgaagg ccacgtccat ggcgttgagc gtgccacttg cgccgactct gcggatgaac
    13441 tcctcattct ggtagccacc gaacaccgcc ccaaggacga cgaggaacca acccgcagga
    13501 tgaatgcccg cgatgcatcc ttggacccgg gcggcgctca tcggctcgag caggtcggcc
    13561 atctcggcaa gggattcctc cgtcctgagc cgctgaaggg tgaccatgca gaggaaatag
    13621 ggcggcatga tgctgaagag tgccagggtg ccaacgaact gtgcctggct catgctggcg
    13681 agaat
//
LOCUS       DBNHLM010000276         7953 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1656994, whole genome shotgun sequence.
ACCESSION   DBNHLM010000276 DBNHLM010000000
VERSION     DBNHLM010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7953)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7953
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1656994"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3399)
                     /gene="dnaE"
                     /locus_tag="AC16QF_12765"
     CDS             complement(<1..3399)
                     /gene="dnaE"
                     /locus_tag="AC16QF_12765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR6449479.1"
                     /translation="MTAVTPASTEGFVHLRVHSEFSVADGLMKVGALADAARRLNMPA
                     VALTDRSNLFGLVKFYQACLSAGVKPLVGADLVYQTDEGGRFRIAVLAMNSQGYRNLL
                     ALVSAAYLDTDERGVLSAGQIFARQEGLLVLSGGRQGEVGQALAKGDLPGAQRIAALW
                     EERFPERFYLELVRTGRGGEDAYIDGAVRLAAELELPVVATNDVCFLAADDFEAHETR
                     VCIYDGRTLDDPRRERSYSSDQYLRTPAEMTALFSDLPEAIENTVQIARRCNVQIELG
                     DYHLPNYPVPEGETLGGFLQRCAREGLERRWQTLAGRPEQAFSFEEYRERLSYELDVI
                     NQMGFAGYFLIVMEFIAWAREHGIPVGPGRGSGAGSLVAYALGITDLDPLGYDLLFER
                     FLNPERVSMPDFDIDFCMEGRDRVISHVSERYGHDAVSQIITFGTMAAKAVVRDVARV
                     QGKPYGLADKLSKLIPFEVGMTLEKAVSQSSELADFIASSEEVGEIMDMAYKLEGIVR
                     GLGRHAGGVVIAPSALTDFVPLYMDDQSGGLVSQFDKDDVESAGLVKFDFLGLKTLTI
                     IDWAVQAVNAARGDADELLDIDHIPLDDPGTFEFLRTAETTGVFQLESSGMKDLIRRL
                     KPDSIDDIIALVALFRPGPLQSGAVDDYIDRKHGQEPITYPHPEMKAVLQNTYGVMLY
                     QEQVMQTAQVLAGFSLGQADLLRRAMGKKKPEEMAKVREQFLSGCSERGVDAALAGQI
                     FDLMEKFSGYAFNKSHSATYALVSFQTAWLKTHHPAEFMAATLSVDMQNTDKVVTLVD
                     ETRNMGLPLSPPSVNRSEYRFSAREGAVVYGLGAVRGVGEGPVQALVEARASGGAFES
                     LPDFCRRVDSRKANRRVLEALIRAGAMDDFAEPGEALDCLRARLMAELGDALHGAEQA
                     ARDVALGMSDMFGGLEEPTLGNGNGRGAPPALGKRERLEWEKEALGLYLSGHPIEDYL
                     GEIREFCGRPIADLEAGKRTQVVAGLVFSARTMRGRRGEMGFVVLDDRSKRIEVSLFS
                     EVFQAGKEKVAKDAVLVVEGEVQPDDYTGELKMRADNVYTIEEARRRFARELVIDCCD
                     ASPTADFSSRLKTCLEPYRDGSQGCSIAVMYQSPATSGPGARGRI"
     gene            complement(3396..4016)
                     /gene="rnhB"
                     /locus_tag="AC16QF_12770"
     CDS             complement(3396..4016)
                     /gene="rnhB"
                     /locus_tag="AC16QF_12770"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008566891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR6449480.1"
                     /translation="MGLFDDLAGTNLTAGVDEVGRGPLAGPVVAGAVVLDPLRPIDGL
                     KDSKRLNARRRAELAEEIRAKALAWGLGRAEVEEIDALNLLRASHLAMQRAVAALSVE
                     PEMILVDGNLLPVFSVPAVAVVKGDARVPEISAGAILAKVARDREMIELDRLYPGYGF
                     AGHKGYPTAEHLSALAELGACAVHRTSFAPVRDLKLRRPTVERLPL"
     gene            complement(4016..5233)
                     /gene="lpxB"
                     /locus_tag="AC16QF_12775"
     CDS             complement(4016..5233)
                     /gene="lpxB"
                     /locus_tag="AC16QF_12775"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HIR6449481.1"
                     /translation="MSWSAGAADTDPAAPRGDICLGILAGEASGDSLGAGLMAALKRR
                     YPRARFVGIGGPRMLAQDLDPVAPMERLQVNGLVDPVKRLPDLVRLLRQLVRCYEASR
                     PAVFIGVDFNVFNFLLERMLKARGIPTVHYVSPSVYAWRRGRVGRIARSADLLLTLFP
                     FEPALYDDAGLEAVFVGHPRADAIDIDAGGAEGRARARRELQIPPDRFCMAVLPGSRL
                     SEVRLMGAPFLDAAAIVARRLPGTLVIVPCLRPEIRTLLEALLAARPELDAVLYDGDA
                     ELALTASDGALVKSGTSTLEAMLLRRPMVVSYKLGPLTYRLIRLLVRTPHIALPNILA
                     GEELVPEVLQDEATPEALAEKFLDQLDKARRDPEHLARFSDLHRQLRMNADERAADAV
                     SRLLDGRAAHTEA"
     gene            complement(5230..6030)
                     /gene="lpxA"
                     /locus_tag="AC16QF_12780"
     CDS             complement(5230..6030)
                     /gene="lpxA"
                     /locus_tag="AC16QF_12780"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HIR6449482.1"
                     /translation="MIDPRAIIDPSAVIGEGVTIGPWTFIGPDVVIGDGCWVAPHVII
                     RGPTRIGRNNKIYQFATVGEDTPAMAYHGEPTTLEIGDDNVIREGVTIHRGMMQDLGR
                     TVIGSHCLLMAYVHVGHDCIVGDHVIMANNSSISGHVRVGDHANFGGYSGVPQFRRIG
                     AYSHISGMSLVLKDVPAYMTVMGNPASAIGLNIEGMRRRGYSKELIDALRSAHRVVYR
                     EGRTIQDACRRLEPAARDWPEVELFLESIRDSRWGIVRPRGRGGETRN"
     gene            complement(6032..6469)
                     /gene="fabZ"
                     /locus_tag="AC16QF_12785"
     CDS             complement(6032..6469)
                     /gene="fabZ"
                     /locus_tag="AC16QF_12785"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR6449483.1"
                     /translation="MKDITEIFDYLPHRYPLLLVDRVLEIVPGERIRGFKNVTFNEDF
                     FNGHFPGNPVMPGVLIVEAMAQLSGVLAFETHDRRPSDGYLYYLASIDNTRFKRPVVP
                     GDRLMMESELVADRRGLMKFKLAAFVDDDLACTAEIACMERKL"
     gene            complement(6530..7573)
                     /gene="lpxD"
                     /locus_tag="AC16QF_12790"
     CDS             complement(6530..7573)
                     /gene="lpxD"
                     /locus_tag="AC16QF_12790"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIR6449484.1"
                     /translation="MSEPAYTLGELADHIQARLVGDANAVINGLGSLATAEAGEVSHL
                     SSPAYRKHLAGTRATALILREADLPLWDGNALVSENPYLSFALVSQLFTRRPELAEGL
                     HPTAEIHPEAVVEPGARLGPGVVVGARTRVSAGARLHAHVVVGEDCRIGEEVTLMPGV
                     VIYPRVKIGARSTAHSGVVIGADGFGFTPSSNGSLEPIAQLGGVSIGADVSIGAGTTI
                     DCGAIEDTVIEDGVKIDNQVQIGHNCHIGAHTVICGCVGIAGSTRIGRHCVLAGKCGI
                     GGGGPVELADGVVLSGGTNVMVSIDRPGVYSGSVLHETHPRWKRNAMRLRRLDDLFRR
                     VADLEKKVKDQTG"
     gene            complement(7579..>7953)
                     /locus_tag="AC16QF_12795"
     CDS             complement(7579..>7953)
                     /locus_tag="AC16QF_12795"
                     /inference="COORDINATES: protein motif:HMM:NF015871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR6449485.1"
                     /translation="QQIQSELQKEQDEVRGLGEEIVALQEKLQKDGEVMSATEQRKVQ
                     KDIEDKQIDYQFLVNKLQKEVNDRRQELLGQMLPKIDKVLKDLIELEGYDMIMQRGNL
                     YYVNSKHDITRKVTEKLNENRQ"
BASE COUNT         1390 a         2687 c         2467 g         1409 t
ORIGIN      
        1 gatgcggccc cgggccccgg gcccggaggt cgccggcgac tggtacatga ccgctatgga
       61 gcatccctgg gaaccatcgc gataaggctc caggcaggtc ttcagacggg aggagaaatc
      121 ggctgtcggg gacgcgtcgc agcagtcgat gacgagctcg cgggcgaagc gccgccgtgc
      181 ctcctcgatc gtgtagacgt tgtcggcccg cattttgagc tctccggtgt agtcgtccgg
      241 ctgaacctcc ccctccacga cgagcacggc gtccttggcg accttctcct tgcccgcctg
      301 gaagacctcc gagaacagcg agacttcgat ccgcttgctg cgatcgtcca gaaccacgaa
      361 acccatctcg ccccggcggc cgcgcatcgt gcgcgcggaa aaaaccagtc cggcgacgac
      421 ctgggtgcgc ttgccggcct cgaggtccgc gatggggcgg ccgcagaact ccctgatctc
      481 cccaaggtag tcttcgatgg ggtggccgct gaggtagagg ccgagcgctt ccttctccca
      541 ctccaggcgc tcccgcttgc cgagcgctgg cggcgcccca cggccgttgc cgttgccgag
      601 ggtgggctcc tccagcccgc cgaacatgtc ggacatgccc agagcgacat cccgcgcggc
      661 ctgctcggcc ccgtgcaacg cgtcgccaag ctcggccatg aggcgcgcgc gcaagcagtc
      721 taaggcttct ccgggctcgg caaagtcgtc catggccccc gcgcggatca gcgcttccag
      781 aacccgccga ttggctttgc gagagtcgac gcggcggcag aagtcgggaa ggctttcgaa
      841 cgcgccgccc gacgcgcgcg cctcgacgag tgcctgcacg gggccctcgc ccacgccccg
      901 cacggcgcca agaccgtaaa ctacggcgcc ctcccttgcc gagaaccggt actcgctgcg
      961 attgacgctc ggcgggctga gcggcagccc catgtttctc gtctcgtcca ccagagtcac
     1021 caccttgtcg gtgttctgca tgtccacaga cagggtggcc gccatgaact ccgccggatg
     1081 gtgggtcttc agccacgcgg tctgaaagga aacgagggcg taggtcgcgc tgtgcgactt
     1141 gttgaaggcg taaccggaga acttctccat aaggtcgaag atctggccgg cgagagcggc
     1201 atcaacgcca cgctcgctgc agccggagag aaactgctcg cgcaccttgg ccatctcttc
     1261 aggcttcttc ttccccatgg cgcgccgcaa caggtcggcc tgaccgaggc tgaacccggc
     1321 gagcacctgg gcggtctgca tgacctgctc ctgatagagc atcacgccgt aggtgttctg
     1381 cagcacggct ttcatctcgg ggtgcggata cgttatgggt tcctgaccat gcttgcgatc
     1441 gatgtagtcg tccaccgcac cggactggag cggaccgggc cggaacaggg ccaccagcgc
     1501 gatgatgtcg tcgatgctgt ccggcttcag ccgcctgatc agatccttca tcccggagga
     1561 ttccagctga aacaccccgg tggtttcggc cgtacgcagg aactcgaagg tccccgggtc
     1621 atccagaggg atgtggtcga tgtccaacag ctcgtctgcg tctccccgcg ccgcgttcac
     1681 tgcctgcacg gcccagtcga tgatggtcag cgtcttcagg ccgaggaagt cgaacttgac
     1741 caggcccgcg ctctccacgt cgtccttgtc gaactgcgaa actaacccgc cgctctgatc
     1801 gtccatgtac agggggacga agtccgtcag cgccgacggc gcgatgacca cgcctccggc
     1861 gtggcgcccc aggccccgaa cgattccttc cagcttgtaa gccatgtcca tgatctcacc
     1921 cacttcctcg ctggaagcga tgaaatccgc aagctcgctg ctctgcgaca ctgccttttc
     1981 cagcgtcatg ccgacttcga acgggatcag cttggagagc ttgtcggcca gaccgtaggg
     2041 cttgccctga acccgggcga cgtcccgaac caccgcttta gcggccatgg ttccgaacgt
     2101 gatgatctgg ctgacggcat cgtggccgta gcgctcggac acgtggctga tgacccggtc
     2161 acggccctcc atgcagaagt cgatgtcgaa gtccggcatg gaaacccgtt ccgggttgag
     2221 gaatcgctca aacagaaggt cgtaacccag ggggtctaga tccgtgatgc ccagcgcgta
     2281 ggcgaccagc gagcctgcgc cggagccgcg cccgggccct acgggaatgc cgtgctcgcg
     2341 cgcccaggca atgaactcca tgacgatcag gaagtagccg gcaaaaccca tctgattgat
     2401 cacgtcgagc tcgtagctca ggcgctcccg gtactcctcg aacgaaaagg cctgctcggg
     2461 acgacccgcc aatgtctgcc atcggcgctc gaggccttcc cgcgcgcagc gctggaggaa
     2521 tccgcccagc gtctcgcctt ccgggaccgg gtagttgggc aggtgatagt cgccgagctc
     2581 gatctgaacg ttgcagcggc gggcaatctg aacggtgttc tcgatcgctt caggcaggtc
     2641 gctgaagaga gccgtcatct ccgcgggcgt tcgcagatac tggtcggacg agtagctgcg
     2701 ttcacgacgc ggatcgtcca gcgttcggcc atcgtagatg cagaccctcg tctcgtgagc
     2761 ctcgaagtcg tccgcggcca gaaagcagac gtcattggta gctaccacgg gcagctcgag
     2821 ttccgccgcc agccgcaccg ccccatcaat gtaagcatcc tcgccgccgc gaccggtgcg
     2881 gacgagctcc aggtagaagc gttcgggaaa gcgctcctcc cacagggccg ctatcctttg
     2941 agccccaggg aggtcgccct tggcaagagc ctggcccacc tcaccctggc gcccgccgga
     3001 aagcaccagc agcccctcct gccgggcgaa tatctggccg gcgctgagca cgccgcgttc
     3061 gtcggtatcg aggtaggcgg cggaaacgag ggccagcaag ttgcgatatc cctgggagtt
     3121 catggccaga acggctatcc ggaaccggcc gccttcatcc gtctggtaga cgagatccgc
     3181 gcccaccaac ggcttcacgc ccgcgctgag acaggcctgg tagaacttca ccagcccgaa
     3241 caggttgctc cggtcggtca gggcgactgc cggcatgttc aagcgccgcg cggcatcagc
     3301 cagggcgccc actttcatca ggccatcggc gaccgagaac tcgctgtgaa cccgcaggtg
     3361 gacgaagcct tcagtgcttg ccggggttac ggcggtcaca gcggaagtcg ctccacggtc
     3421 ggccgacgga gcttgagatc gcggaccggc gcaaagctcg tccggtggac ggcacacgca
     3481 cccagctccg cgagcgcaga gaggtgctcg gcggtcggat agcccttgtg gccggcaaag
     3541 ccgtaacccg gataaagacg atccagttcg atcatttccc gatcacgcgc caccttggcc
     3601 aggattgctc cggcgctgat ctccgggacg cgggcgtccc ctttgaccac cgcgacggcg
     3661 ggaaccgaga agaccggcag caggtttcca tcgacgagga tcatctccgg ctccacggaa
     3721 agagccgcta ccgcccgctg catcgcaaga tggctggccc ggagtaggtt gagcgcatcg
     3781 atctcctcga cctccgcgcg tccgagcccc cacgcaaggg ccttagcccg gatttcctca
     3841 gccagctcgg cccgccgcct ggcgttcagc cgcttggagt ccttgaggcc gtcaatgggc
     3901 ctcagcggat cgagcaccac ggcgccagcc acgaccggac cggcgagggg cccacgccct
     3961 acctcgtcta cgcccgcggt gaggttagta ccggcgagat cgtcgaatag tcccatcaag
     4021 cctcggtatg cgcggcgcgg ccgtccagca gccggctcac ggcatctgcc gccctttcat
     4081 cggcgttcat tctgagttgt cggtgcaggt ctgagaaccg ggctaaatgt tcagggtcgc
     4141 gtctggcttt gtccagttga tcgaggaatt tttccgcgag cgcctcgggg gtggcctcat
     4201 cctgaagcac ttccggcacc agttcctccc ctgcaaggat gttcggcagc gcaatatggg
     4261 gagttctcac cagcagccgg atcaaccgat aggtcaaggg gccaagcttg tagctcacca
     4321 ccattggccg gcgcagcagc atcgcctcca gcgtgctggt gccggacttg accagcgcgc
     4381 cgtccgaggc cgtcagtgcc agctcggcat cgccgtcgta gagcaccgcg tccagttcgg
     4441 gccgggcggc cagcaatgcc tccagcaatg tgcggatctc cgggcggagg cacggcacga
     4501 tcaccagggt gccgggcaga cgccgggcga cgatggctgc cgcgtcgaga aacggcgcgc
     4561 ccatgagccg gacctcggag agccggctgc cgggcagaac cgccatgcag aaccggtcag
     4621 gagggatctg gagttcgcga cgagcccgcg cccgcccttc ggcccccccc gcgtcgatgt
     4681 cgatggcgtc tgcccgggga tggcccacga acaccgcctc gagaccggca tcgtcataca
     4741 acgccggctc gaacggaaac agggtgagga gcagatcggc agatcgggcg atgcgcccaa
     4801 cgcgcccgcg gcgccaggcg taaaccgagg ggctgacata gtgcacggtc gggatgcctc
     4861 gagccttgag catccgctcc agcagaaagt tgaacacgtt gaagtcgacg ccgatgaaca
     4921 cagccggacg ggaggcctcg tagcaacgca cgagctgccg cagaaggcgg accagatcag
     4981 gaaggcgctt tacggggtca accagcccgt tgacctgcag gcgctccatg ggcgcgaccg
     5041 gatctagatc ctgcgccagc atccgcgggc caccgatgcc gacgaaacga gcgcgcgggt
     5101 agcgtctctt gagcgcggcc atgagccccg ctccgaggct gtccccggat gcctctccgg
     5161 cgagaatacc cagacagatg tcgccgcgcg gcgccgccgg atcggtgtcg gcagctcccg
     5221 ccgaccagct caatttcggg tttcgccgcc gcgaccccgg ggacggacga tgccccatcg
     5281 cgagtcccga atggattcca ggaacaactc gacctcgggc cagtcccggg ccgccggctc
     5341 caggcgtcga caggcatcct gaatggtcct gccctcgcga tagacgaccc tgtgcgcgct
     5401 gcgaagcgcg tcgatgagct ccttggagta accgcgccgc cgcatccctt cgatgttcag
     5461 gccaatggca gaggccggat tgcccatcac cgtcatgtag gccggcacgt ccttcagcac
     5521 caggctcatg cctgaaatgt gggagtaggc gccgatccgg cgaaactgcg ggacgcccga
     5581 gtaaccgccg aaattggcat gatctcctac cctcacatgc ccggagatgg acgagttgtt
     5641 cgccatgatc acgtgatcgc ccacgatgca gtcatggccc acgtgcacgt atgccatgag
     5701 cagacaatgg ctgccgatca cggttctgcc gagatcctgc atcatgccgc gatgaatggt
     5761 gacgccctcg cggatcacgt tgtcgtcgcc gatctccagg gtggtgggct ccccgtgata
     5821 cgccatggcc ggcgtgtcct cgcccaccgt ggcgaactgg taaatcttgt tgttgcggcc
     5881 gattcgggtt gggcccctga tgatcacatg gggcgcgacc cagcagccgt cgccgatcac
     5941 cacgtcgggg ccgatgaacg tccacggacc gatagtgacg ccttccccga tgaccgcgct
     6001 cgggtcgatg atggcgcgcg ggtcgatcac ctcagagctt ccgttccatg caggcgatct
     6061 ccgcagtgca ggcaagatcg tcgtccacga acgccgcgag cttgaatttc atcagccccc
     6121 gccggtctgc caccagctcg ctctccatca tcaggcggtc accgggcacc accggacgct
     6181 tgaatcgggt gttgtcgatg ctcgccagat agtagaggta gccatccgaa gggcgccggt
     6241 catgggtctc gaaggccagc acgcccgaga gctgcgccat ggcttcgacg atgagaacgc
     6301 ccggcatcac ggggttgcct ggaaagtgac cgttgaagaa gtcttcgttg aaggtcacgt
     6361 tcttgaatcc ccgaatccgc tcccccggga cgatttccag cacgcggtcc acgagaagca
     6421 gcgggtagcg atgggggaga tagtcgaaga tttcggtgat gtctttcatg ccgatgacca
     6481 cgttctttgt cgggtttgcg ctgatcgacc cgaaagccaa aactgccgat cagcctgtct
     6541 ggtcctttac cttcttctcg aggtccgcca ctcgccggaa cagatcgtcc agccggcgca
     6601 gccgcatggc gttccgcttc cagcgcggat gggtctcgtg cagcaccgag cccgaataca
     6661 caccgggccg atcgatggaa accatcacgt tggtcccgcc actgagcact accccatcgg
     6721 ccagctccac aggcccgcct ccgccgatgc cgcatttgcc ggcgagcaca cagtgtctgc
     6781 cgattcgtgt gcttcccgca atccccacgc acccgcagat cacggtgtgg gcgccgatgt
     6841 ggcagttgtg gccgatctgc acctggttgt cgatcttgac cccatcctcg atgaccgtgt
     6901 cttcgatggc gccgcagtcg atggtggttc ccgcgccgat gctgacgtcc gcgccgattg
     6961 acacgccgcc gagctgggca atgggttcca ggctaccgtt tgatgacggc gtgaacccga
     7021 aaccgtcggc cccgatcacc acgccgctgt gggccgtgct gcgcgcgccg atcttcacgc
     7081 gagggtagat cactaccccc ggcatcaagg ttacctcctc gccgatccgg cagtcttccc
     7141 ccaccacgac gtgggcgtgc agcctggcac ccgcggacac ccgggttctc gcccccacga
     7201 ccacgcccgg gcccagccgg gcaccgggct cgaccaccgc ctcgggatga atctccgctg
     7261 tggggtgaag tccctccgca agctcgggtc gccgggtgaa gagctgtgaa accagggcaa
     7321 atgacaggta tgggttctcc gagaccaggg cgttgccgtc ccacaacggc agatctgcct
     7381 cccggagaat cagcgcggtt gcccgcgtac cggccagatg ttttcggtac gcggggctcg
     7441 aaagatggct cacttcgccc gcctcggccg tcgcgaggct ccccagcccg ttgattaccg
     7501 cattggcgtc gccaacgagg cgggcctgga tgtggtcagc gagctctcca agcgtatagg
     7561 ccggctcgct cattcggtct actgacggtt ctcgttcagc ttctcggtga ccttgcgtgt
     7621 gatgtcatgt ttcgagttga cgtagtagag gtttccgcgc tgcatgatca tgtcgtagcc
     7681 ctcgagctcg atcaggtcct tgagcacctt gtcgatcttg ggcaacatct ggccgagcag
     7741 ctcctggcgt ctatcgttga cttctttctg cagcttgttg acgaggaact ggtaatcgat
     7801 ctgcttgtcc tcgatgtcct tctgcacctt gcgctgctct gtggcgctca tcacctcgcc
     7861 gtctttctgc agtttttcct gcagcgcaac aatctcctcg ccgagacctc tcacctcgtc
     7921 ctgctccttc tgcagctccg actgtatctg ctg
//
LOCUS       DBNHLM010000277        20972 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_579844, whole genome shotgun sequence.
ACCESSION   DBNHLM010000277 DBNHLM010000000
VERSION     DBNHLM010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 20972)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20972)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20972
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_579844"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="AC16QF_12800"
     CDS             <1..307
                     /locus_tag="AC16QF_12800"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6449486.1"
                     /translation="AELAFADLRGSQWFGYVFVARAYRGEPVETAEARPSWHPLNAIP
                     YGRMWEDDRVWLPRVVAGERVRGRFLFRAGRLLAHALEGLGEVDGSDAEHRGGCHGR"
     gene            297..923
                     /locus_tag="AC16QF_12805"
     CDS             297..923
                     /locus_tag="AC16QF_12805"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6449487.1"
                     /translation="MAADLNWLDSCRRLRAIAQTGLAYSRDPYDRDRFQELRLIAERM
                     LAWLSDAHPDRVAEVFLPERGYPTPKIDVRAGVFRGDSVLLVKERADGRWALPGGWGD
                     EGDSPRSSIEREVLEESGYEVRAARLVAIKDRHLHPYEKHSMLRVYKLFFLCDLLGGE
                     PRASLETESAEFFPITRLPELSLGRTLPDDVHLLNAYRAEPDRDVYLD"
     gene            complement(1045..2352)
                     /locus_tag="AC16QF_12810"
     CDS             complement(1045..2352)
                     /locus_tag="AC16QF_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase family protein"
                     /protein_id="HIR6449488.1"
                     /translation="MTDLNWPFLGGAALEIESASGVHLTTRDGRRILDAAGGAIVVNI
                     GHGRRRVADRIAEAALTCSYVVPPWVTPSRRAMVEALEANWLDAAFTRLHFTSGGSEA
                     VEAAMKLALQYHAARGHGSRNQILSRAVSYHGTTVATTAVSGHAARRRGLEHGLPRPP
                     TIPTPDALRCPLGANHPDAGAYYAGAVREAIVEAGPENVAAFLAEPITGASGGAIVPP
                     ADYWPRVSEICREFGVLLILDEVMTGFGRTGTNFAYQQWEILPDILIAGKGLAGGYAP
                     LGGVFATEPVGAAIGAAGLQVMFHTFAAHPTACAAAAEVLDILVEEQLVARADRMGTM
                     LLERLQDAFREHPHVSDVRGRGLLQAIEIVSDRATLTPFPMEAQITRRVVDAALERGV
                     FFYAGGTGEMRDIICLGPPFVIEERHVDEMVSVLRQSVDAVTR"
     gene            complement(2357..3994)
                     /locus_tag="AC16QF_12815"
     CDS             complement(2357..3994)
                     /locus_tag="AC16QF_12815"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449489.1"
                     /translation="MTRHLLFAAAGLLLAACSTVSIDGPTAPRGDPAAGRLTLPSGRT
                     VWVYPNDLAFTGQARYEWPDGRAYAGGWLSGRPHGTGLMRSDDGERYSGMWNEGRRHG
                     HGELTNPNGDHYVGSFVNGLREGAGTQKSQDGFYRGEWAGDLPNGEGRFNANDGSLYQ
                     GQWLDGERAGTGTYTDASGNRYEGEWLADVPHGFGILTEQDGSEYDGRWTAGSRDGYG
                     ASRNALDMAYEGTWNAGNYHGFGILLRPDGSSYEGEWHHGKRQGQGRETDPEGGFHDG
                     AWENNQPLGPGTRRDVAGVEIAGMWTGSRVRTGLVKLPTGLEYAGPLFKDRNRGVSAP
                     LQEWLLTQASRGDPYAQLYLGRAFDDLGGTSADAARAAQWYALAAAAGVAEARYRLAL
                     LNIEDNPPRAVELLAAAAGQGHARANAALADCYLAGKIVPRNAARAIAYLETAMDAGS
                     VAARNTLARVLATTTEDGLRDGARAVALIRPLALYTESWWQLDTLAAALAETGAYGDA
                     VTALQLALERADQISPRDVQQLVDHLAAYRAETPLRE"
     gene            4073..5107
                     /locus_tag="AC16QF_12820"
     CDS             4073..5107
                     /locus_tag="AC16QF_12820"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6449490.1"
                     /translation="MTASKDDGSIEGVDIPRVTHWLAGRIAGLRPPLEFSLIAGGHSN
                     LTYRFADQAGAEYVLRRPPLGHVLESAHDMAREHRIVSALAGTEVPVAPTHGLCEDRD
                     VNGAPFYVMSYVRGAVLHDADAARELDPAERHALGEDVINVLARLHALDPDAVGLGTL
                     GRKEAYLARQLKRWKKQWEASKTHEIPEMEETARLLEENMPEQIGAAIVHGDYRLGNM
                     IVADGRILAVLDWELCTLGDPLADVGYLLNNWIQPEDGAGELVPTAAGGFPSREALCD
                     GYTAATGRDLSGINYYRAFSHWRLAAIGQGVYKRYLVGAMGERPDMDLDTYKASVQQR
                     AAAALELLTA"
     gene            complement(5104..5574)
                     /locus_tag="AC16QF_12825"
     CDS             complement(5104..5574)
                     /locus_tag="AC16QF_12825"
                     /inference="COORDINATES: protein motif:HMM:NF025701.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HIR6449491.1"
                     /translation="MTRSRTILGALLVAALYGCSGRDDVSLVEVLNHDQCRMSQAGTE
                     VVSIEDVARIRGGRLLSAPDEPATTPTDLLLVAVSRGQQPTPGYGFELVAGTMKGDAV
                     TVRLAWNQPPADAVLAQVITNPCIVIGLEGEGFSRLRVIDQDDTPLGEIVLPGG"
     gene            complement(5571..8438)
                     /locus_tag="AC16QF_12830"
     CDS             complement(5571..8438)
                     /locus_tag="AC16QF_12830"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR6449492.1"
                     /translation="MIRQALPKTVWTVTCVLTLTACGGGGGGSDTPTPAPANRSPAAS
                     FTATPTEGTVPLSVDFDASGSSDADGRIASYAWTFGEPPGASSTSGVNTSHTYEEAGT
                     YTVELTVTDDDGATGSTTRTVTVEGPVASISGTVRILASSAIDSDVNDVGSTPAPNND
                     FASAQPLPNPVTLGGFANVAGVGVGDEPERGKLFQDGDPGDFYAMTMVGNEFIYLNIA
                     DPENAEQELLLYDSQQNVISADGGPGEVQVAEVPAAGDYFIEVRPIAGASNYVLTVGQ
                     DLVPERATTMIDPFLPREIILRTTDDEIAAEYGLSMLAQSGPRMLLKAGPATAALRHI
                     HRRAPFDASWTEDQQDRYATLAVIEALGRDPRVELAEPNLIRKPHREPGDSFYRSQWH
                     YQAINLPLAWDITTGDGDVIVAVVDTGIRADHPDLDEKVIAGFDFIANLQRAADGDAR
                     DDDPTDPGDGAFGGSSSFHGTHVAGTVAAETSASVTPASGVAGVAWEAKIMPLRVLGV
                     GGGTSFDVIESMRWAARLDNVSGTLPDRAADVINLSLGGGGFLESEQQAIDEIRAAGT
                     IIVASAGNDASASPSYPAAYDGVVSVSATTIENNPAAYSNFGTTVDVAAPGGSNVTDV
                     NGDGIGDGVISTMADDSDPSSLRFGYASLQGTSMAAPHVAGVVALMKAVHPGLTPIQF
                     DNALASGRITDDLGAPGRDDRYGWGLINAQKAVLAALDLASNPPGPTLAVSASSLTFG
                     AALSSQSLRLENVGSGTVTVQSIGTAASWLDVAPSSVLPGGMGSYTVTVDRTGLTPGS
                     YTGAIEIVTDAVNPTATVTATMRVFTTSPDANAGVHYVVLVDAAGETLPSPQNVLEAT
                     NGEYAYRLDGVAPGDYRIFAGTDSDDDAFLCDAGEACGTYGTLDSPAVVTVDGEDLTG
                     IDFTSEFRVNLTNLSANADVSGQVAPEAVRLPGKADGGAPR"
     gene            complement(8875..9777)
                     /locus_tag="AC16QF_12835"
     CDS             complement(8875..9777)
                     /locus_tag="AC16QF_12835"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6449493.1"
                     /translation="MVSRSAIDDDLYRIGTVATLTGISVERLRAWERRYGLAPTYRSG
                     KTRFYSKQQLQTLQRIKRLIDQGHPISSLAPLSDEQLDERLSAATPRLQALQSATVGL
                     IGPNLLVLEQQQGSDNRLEVRARWANMDAFMSDQTGTERIDVIVAQLPVLLVDHINNL
                     ARFHPESRIVAIYQFATPAQIAAVQELGIPTLTWPAGWQEIEHACATTAGLPLRAART
                     AVRRFSDAELIAISASEHGDPAACPQHLVELISGLNAFAEYTLNCADEAAEPEVYQRV
                     HTDTTQARAQLELALEMMVDQEAG"
     gene            10203..11750
                     /locus_tag="AC16QF_12840"
     CDS             10203..11750
                     /locus_tag="AC16QF_12840"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0032787 - monocarboxylic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6449494.1"
                     /translation="MENNLGLFLTKRAFLTPQREGYVDSHSGLRLTFRQLNERSNQLA
                     NSLLQAGVQKEERVGLLLMNSAEFMEAYFALAKIGAVVVPLNWRLVADELEFILKDSG
                     TARLIFDEDFVDTVAELHARGDRTDIRHWLQVEGSQEVAHFAESYEAFRDNGAAEEPP
                     CTACDDDMLYIMYTSGTTGLPKGVVHTHTTAIWAILTIAANAYYEETDRYLGALPMFH
                     VGALTPLAVNVYRGATSVVMRAFDPVLAWELIDSERITSGLMVPAMLNFMLQVGEAKD
                     RYDYSALRWIMTGAAPVPVALTEQYVQMGISILQVYGLTESCGPACLMDSENALRKPD
                     STGKAFFHTDVRVVDAHGNDCAPGEAGEVVVRGKHIMREYWNRPDATTETIVDGWLHT
                     GDVAVMDEEGFVSIQDRIKDMIISGGENVYPAEIEGVLISHPKISEAAVIGQPSERWG
                     ESPLAIVVKADPDLDVAEVMEFCRGKLAGYKQPKGVEFVDEIPRNPSGKILKRILREQ
                     FPGPAPA"
     gene            complement(11796..12179)
                     /locus_tag="AC16QF_12845"
     CDS             complement(11796..12179)
                     /locus_tag="AC16QF_12845"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HIR6449495.1"
                     /translation="MTKPGYTGIKRIRKAAGYSSQGIRAAWQHESAFRQECTVALVLL
                     PCAVLLAETWMQLAVLITVCVLVLIAELLNSAVEAVVDRIGHEHNDLAGQAKDMASAA
                     VALAIFLVPLIWGLVALERFVGYWI"
     gene            complement(12194..13075)
                     /locus_tag="AC16QF_12850"
     CDS             complement(12194..13075)
                     /locus_tag="AC16QF_12850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR6449496.1"
                     /translation="MGHDHNHIHTRDNEVIVGRAFVLIAGFMVVEVIGGLLANSLTLL
                     ADAGHMFLDATALGLSWYALRLSKRSNDHKLSYGYHRFQVLAAFINGLTLMALVVWIL
                     VEAYARLRAPEGMLALPALAVAVVGMVVNLIAFRWLHGAEDNATVRSAALHVIGDLLG
                     SAAAIIAALTVYLTGWLYADPLLAVVIVVILGRGAWRVLKESAHILLEGVPHGINLTE
                     IAETLAARVPGVIEVHHVHAWALTSEKPLVTLHASVDEGTELAGVVSRMKVVLNDDFG
                     IDHSTIQVEHGPCPDDD"
     gene            complement(13190..14335)
                     /locus_tag="AC16QF_12855"
     CDS             complement(13190..14335)
                     /locus_tag="AC16QF_12855"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449497.1"
                     /translation="MNPVETAAGFAAEIRRRAPEIEQHRRMPGDLANRMAGAGLFRLL
                     VPELYGGLQVEPLAFFETLKETARADGAVGWCQMIGVTTGMMAASLPDEWSRTIYGCN
                     PDTITSGVTAPLGIAVAEGDGLRVSGRWPFGSGCEVSDWICGGCQLMEDGEPRKNRFG
                     SPEIALVFFPADDVTIHDTWHTSGLCGTGSHDIEVKDAEVPAGRWVTMGKRARVDAPL
                     YRFPTLGLLALGVSAVALGIAEHAIEAFTTLARDKVPTGSARPLSARSGAQRDLARAM
                     AAVQSAAALTREAIEDAWGEAHREGSLGLETKARLRLAATNNAWSAAEAVDLLYHAAG
                     GSSIYAHSALQRCFRDVHVATQHLMVAQPIYEVVGKVALGIDPKTLL"
     gene            complement(14332..14811)
                     /locus_tag="AC16QF_12860"
     CDS             complement(14332..14811)
                     /locus_tag="AC16QF_12860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6449498.1"
                     /translation="MAELKVCDVKLAAIRDAGLIARMSRDYIEHGLRWRWRAPQIRAK
                     IRHPETAVIVAEVELGGVRFIGGFASMDFGEDKAALNLLAVHPNLRRRGIGGNLVEWL
                     HKSAGVAGCQHIELQVRAGNVSARRFYGRMGYTEEALINGYYDGKEAAYQMKFRLAQ"
     gene            complement(14889..15338)
                     /locus_tag="AC16QF_12865"
     CDS             complement(14889..15338)
                     /locus_tag="AC16QF_12865"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6449499.1"
                     /translation="MDNQLIAEIQRDCEALSLAYARAIDFRDYDHFVSLFTDDAVLDL
                     GRPLRGREEIHAAISARPDELRSRHVITNVFVDVVDENTARGIAYLTLYRHIADKPSI
                     PGPGSEPAPTTLPAAVGHYQDRFARGPDGWRFEARSLHLAFRDPAAF"
     gene            15443..16225
                     /locus_tag="AC16QF_12870"
     CDS             15443..16225
                     /locus_tag="AC16QF_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011533963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6449500.1"
                     /translation="MERMSLEFVGKVAVLKFNHPEVMNAVGSQMLLDFADALKDIAHP
                     GNGARCLLLTGEGRGFCAGANLQDGERSERRGGAGDGLRASYHPILLTLRDLNMPMVT
                     AVNGAAAGVGMSFAMMGDIVCASSNAFFLQAFARIGLVPDGGSTFLLPRLVGWGRAME
                     LSMLAERLPAEKAHEWGLVNRLFDDNAALLEGAMAIADRLASGPRSLALIRQAYWNTW
                     HNAFEQQLDLEARLQNQAGASRDFAEGVAAFLEKRDAEFTGE"
     gene            complement(16229..17146)
                     /locus_tag="AC16QF_12875"
     CDS             complement(16229..17146)
                     /locus_tag="AC16QF_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR6449501.1"
                     /translation="MLLITHRDCLLHEMQHRHPERPDRLRAVLQLLESSGLSRDLKHA
                     EALPASQEALRAVHDPDYLRTLSESVPESGLLPVDPDTAMGPSSLRAAALAAGAVADG
                     VRAVLEGDQSTVFCAVRPPGHHAEHDAAMGFCFYNSIALGAVTALEDPRVERVAVLDF
                     DVHHGNGTVDIFKDRPEVLVCSSFQHPFYPHRYADLARPNIVNTPLPAGTAGADFRRA
                     VEAQWLPAIDNHQPQLIMVSAGFDAHRLDPLAQLELCEDDFRWITRLIVASAKTHANG
                     RIVSALEGGYDLAALAASAHAHIEELLAA"
     gene            complement(17187..18767)
                     /locus_tag="AC16QF_12880"
     CDS             complement(17187..18767)
                     /locus_tag="AC16QF_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR6449502.1"
                     /translation="MIVCALIKDEQLRDRLRRLGARAGWRVLFAADDRDLLVTLISGK
                     ATPDVIVSDEPDNLTGVGALPTTRIYLRPDRGEPGTDAFDPALDDQALLQHINNCVNA
                     RRFRARFAAIDRNEPITSLPRHEELFDALSRHVDEPVGMMVVKLDHAEHLYGSLDPVA
                     KTDLLAALSQRLESKLPTQARLGFYDATCFVVTLPRIQSSELERLGNDLRLAVSAPLI
                     DAGGEIHLTISIGCSFASSFNGADHLWASAWAAANAASQAGGDRVLGANPDTAISERI
                     PSALERDEFSLLLQPQWSLQDNRLTAVEALLRWQGRDIGGLAPSHFIPLVEQQGQMAR
                     VGDWVLERACRETAAWLPRMVQPILLTVNVSPQQFHGRSIVTQIARNSAEHRLNPASL
                     ELELSQRNMLRLVDDYREQMYTLRDWGVRFAIDNLGGGLIEPEKLLRCPVDTLKIDRA
                     VVSALSEDTQAADLAAQICELGARFNLRVVGVGVETEEQLKLLAEWGCTDAQGLLVSR
                     PVPVTGIQHLMNRLADLN"
     gene            complement(18817..>20972)
                     /locus_tag="AC16QF_12885"
     CDS             complement(18817..>20972)
                     /locus_tag="AC16QF_12885"
                     /inference="COORDINATES: protein motif:HMM:NF020083.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lhr family helicase"
                     /protein_id="HIR6449503.1"
                     /translation="LEQSGFFGARFRDCAGRALLLSKQRFNQRLPLWMSRLQAKKLMS
                     ATAGYPDFPMLLESWRTCLKDEFELDSLRQMLTELEDGTIEWTFVSTSSPSPFAANVT
                     FAQINRYMYADDTPEEDAGPSALSDDLIRSAVRNAGLRPPIRREIIQEYLAKRQRTAE
                     GYRPVSAEDWSEWVKERVLIPAAEVRAATSADAPVPEHEHLVWLEERAGSGENRAWLT
                     HRELLHGLAQRGLRDGLAFQGTVPDVEESRSAEQLALEILSFYGPLTESDIAWILPRV
                     PESLLDENEVLVSGALVADDPGLYYCDAENYAALLRFQRAAARPELQPRPFHTLPGFL
                     ASWQRFGVSGGDEHVLEVMEPLRGFRAPVRAWLEDVVSARIPAMADSQLDDVLLRHGM
                     QWRGAGRKAIAIGYPEDLQLIDDGVDEPSTLAKLFTDPTARYPFVQLADRSGLSGEAF
                     NEQWWASVWEGEITSDTLAPLRQGLERGYALNAAAPQRASSHRQGRRRARPAPAWTGN
                     WSVKPPFDADIDPLTELEDAKDRARMLLDRYGIICRELANREGRLLRWRCVFRALRLM
                     ELAGEVTAGYFFTGLSGPQFVSPGALRRLQQGWQTPEDFWISAVDPASPCALVTAIPD
                     LPARRPNNYLAFHEGSLALVIENAGKRLQFHRGPDDACLGRVCEPLAHLVRRRRRVEV
                     ELINDVPARSSPYLEPLGRFFHAVTDHKQLILMIG"
BASE COUNT         3438 a         6695 c         7206 g         3633 t
ORIGIN      
        1 cgccgaactc gcgttcgccg atctgcgcgg cagccagtgg ttcggctacg ttttcgtcgc
       61 gcgcgcgtac cggggagagc cggtggagac agccgaggcc aggccgagct ggcatccgct
      121 gaacgcgatt ccctatggcc ggatgtggga ggacgatcgg gtctggttgc cgcgggtggt
      181 tgccggcgag cgcgttcgtg gccggttcct cttccgcgct ggtcggttgc tcgcccacgc
      241 gctggaaggc ctcggtgaag tggatggcag cgatgccgag caccggggag gctgccatgg
      301 ccgctgatct gaactggctg gacagctgcc ggcgcttgag agccattgcc cagacgggac
      361 tcgcttacag tcgggatccc tacgatcggg atcgatttca ggagctgcgc ctgatcgccg
      421 agcggatgct ggcctggctg tcggatgcgc accccgatcg cgtcgctgaa gtattcctgc
      481 cggaacgcgg ctatcccacg ccgaagatcg acgtgcgcgc cggcgtgttc cgcggcgaca
      541 gcgtcctcct ggtcaaggag cgcgccgacg gccggtgggc cctgcccggg ggatggggcg
      601 acgagggcga ttcgcccaga tcctccattg agcgcgaggt gctcgaggag tccggctacg
      661 aggtcagggc agcacggctg gtcgccatca aggaccggca tctgcacccc tacgaaaagc
      721 actcgatgct cagggtgtac aagctctttt ttctgtgcga tctgctcggg ggagagcctc
      781 gcgccagcct ggaaaccgag tcggccgagt tcttcccgat cacccggctg ccggagctca
      841 gcctcggcag aacgctgccg gacgacgttc acctgctcaa cgcctaccgg gcggaaccgg
      901 accgcgacgt ttacctggac tgagcccatg ggtaatcgct cgtgcgccgg cgcgattgct
      961 cctccaaatt agtcgggccg gcgcgtctgg tccgttacat cgaccggcac ttctgctgat
     1021 acgcgaacgg gccgagcgtg tcgcctatcg ggtcacggcg tcgacggact gccgcagcac
     1081 cgagaccatc tcatcgacgt gtctttcctc tatgacgaac gggggcccca gacagatgat
     1141 gtcgcgcatc tcaccggtac ccccggcata gaagaagacc ccccgttcca gcgccgcgtc
     1201 gaccacccgc cgggttatct gggcttccat cggaaacggc gtgagggtcg cccggtcgct
     1261 gacgatctcg atggcctgga gcaggccccg accccgcaca tcggacacgt gggggtgctc
     1321 gcggaaggca tcttgaagcc tctccagcag catcgtgccc atgcgatcgg cgcgcgcgac
     1381 cagttgctcc tcgaccagga tgtcgagcac ctcggcagcg gccgcgcacg cggttgggtg
     1441 ggctgcgaag gtgtgaaaca tgacctgcag cccggccgcg ccgatggcgg cgcccaccgg
     1501 ctccgtggcg aaaacaccgc ccagcggagc atacccgccg gcaagcccct ttccggcgat
     1561 gaggatgtcc ggaagaatct cccactgctg gtaggcgaag ttcgttcccg tccggccgaa
     1621 gcccgtcatg acctcgtcca gaatcagcag cacgccgaac tcccggcaga tctcgctcac
     1681 cctcggccag taatccgcgg gaggcacgat ggcgccgccg ctcgcgccgg tgatgggttc
     1741 ggccaggaat gccgcgacgt tttcaggccc tgcctcgacg atggcctcgc gcacggctcc
     1801 ggcgtagtag gcgcccgcgt ccggatgatt ggcgcccagc gggcagcgca gggcgtcggg
     1861 ggtcgggatc gtcggaggtc gaggcagccc gtgttcgagg ccgcgtcttc tcgccgcgtg
     1921 accgctgacc gcggtagtcg cgacggtggt cccgtgatag gacacggcgc gtgacaggat
     1981 ctgatttcgt gacccgtggc ctcgggcagc atggtattgc agcgcgagct tcatggcggc
     2041 ttccacggcc tcggaaccgc cggaagtgaa atgcagcctc gtgaaagccg cgtccagcca
     2101 gttggcttcc agcgcctcca ccatggcacg ccgggagggc gttacccagg gaggcaccac
     2161 gtagctgcag gtcagcgccg cctcggcgat gcggtccgct acccgccgtc ggccgtgtcc
     2221 gatgttcacg acgatcgcgc cgccggcggc atcgaggata cgcctgccgt cccgggtcgt
     2281 cagatggacg ccgctggcac tttcgatctc caaggcggcg ccgccaagga acggccagtt
     2341 gagatcggtc atgggctcac tctcgcaagg gtgtttccgc ccggtaggcg gccagatggt
     2401 ccaccagctg ctgcacgtcc cgcgggctta tctggtcggc ccgctccagg gccagctgca
     2461 acgcggtcac cgcgtccccg taagcaccgg tctccgccag cgcggcggcc agggtgtcga
     2521 gctgccacca gctctcggtg tagagcgcca agggacggat cagcgcgacg gcccgggcgc
     2581 cgtcgcgcag gccgtcctcc gtggtggttg cgagcaccct ggcgagggta ttgcgtgccg
     2641 cgacgctgcc ggcgtccatg gccgtctcca ggtaggcgat ggccctggcg gcgtttctgg
     2701 ggacgatctt gcccgccagg taacagtcgg ccagcgcggc gttggcccgg gcgtggccct
     2761 ggccagcggc tgcggccagc agctcgacgg cgcgcggcgg gttgtcttcg atgttgagca
     2821 aggccagccg atagcgcgcc tcggcgacgc ccgccgccgc tgcgagggcg taccactggg
     2881 ccgcccgggc cgcgtcggcc gacgtgccgc ccagatcgtc gaacgcgcgg ccgaggtaga
     2941 gctgagcata ggggtcaccg cgcgacgcct gggtgagcag ccattcctgg agcggcgcgc
     3001 tgacgccgcg gttgcggtcc ttgaaaagcg gaccggcgta ctccagcccg gtgggcagct
     3061 ttacgagccc ggtccgcacc cggctgccgg tccacatgcc ggcaatctcc acgccggcga
     3121 cgtcgcgacg cgtacccggg ccgaggggct ggttgttctc ccaggccccg tcgtggaagc
     3181 ctccttcggg gtccgtctcg cggccctgac cctgccgctt gccgtgatgc cactcgcctt
     3241 cgtagctgct gccgtccggg cgcagaagaa tgccgaagcc gtgataattg cccgcgttcc
     3301 acgtgccctc gtaggccata tccagggcgt tccgcgaagc tccatagccg tcgcggcttc
     3361 cggccgtcca tcggccgtcg tattcggatc cgtcttgctc ggtgagaatg ccgaagccgt
     3421 ggggcacgtc ggcgagccat tccccttcgt agcggttgcc gctcgcgtcg gtgtacgtcc
     3481 cggtgccagc acgctcgccg tcgagccact ggccctggta gaggcttcca tcgttggcat
     3541 tgaagcggcc ctcgccgttc gggagatcgc ccgcccattc gccccggtag aagccgtcct
     3601 gggatttttg ggttcctgcg ccttctcgca ggccgttgac aaagctgccc acgtaatgat
     3661 cgccgttcgg gttcgtcagc tcgccgtggc cgtggcgtct gccctcgttc cacatgccgc
     3721 tgtagcgctc accgtcgtcg ctgcgcatga gcccggtgcc gtgtggcctg ccggacagcc
     3781 agccgcccgc ataggcgcgc ccgtcgggcc attcgtagcg agcctgcccg gtgaatgcca
     3841 gatcgttcgg gtagacccac acggtgcgcc cggagggcag cgtgagcctg ccggcagcgg
     3901 gatcgcctcg gggggcggtg ggaccgtcta tggagaccgt agagcatgcc gccaggagca
     3961 gacctgcggc agcgaagagc agatgtcgcg tcactgggtt atggaaaacg atctcatgtc
     4021 aaaactcaaa ggctttgggt aaccttgccc gggtctggaa cgggagaatt ccgtgaccgc
     4081 gagcaaagac gacggcagca tcgaaggcgt cgacatcccg cgcgtgaccc attggctggc
     4141 aggccggatc gccggcctgc ggccgccgct ggagttttcg ctgattgcgg gcggtcactc
     4201 gaacctcacc taccgctttg ccgatcaggc tggcgccgag tacgtgctgc gtcgcccgcc
     4261 cctgggccac gttcttgaaa gtgctcacga catggcccgg gagcatcgta tcgtctccgc
     4321 gctcgcgggc accgaggttc ccgtggcgcc cacccatggc ctttgtgagg accgggacgt
     4381 caacggggcg cccttctacg tgatgagcta cgtccggggg gccgtgctgc acgatgcgga
     4441 cgccgcccgt gagctcgatc cggccgagcg gcatgccctt ggcgaggatg tgatcaacgt
     4501 gctcgccagg ctccatgccc tggatcccga cgcagtgggt ctcggcacgc tggggcgcaa
     4561 ggaagcctac cttgcccggc agctcaagcg ctggaagaag cagtgggagg catcgaagac
     4621 ccacgagatt cctgagatgg aggagacggc caggctgctc gaggaaaaca tgcccgagca
     4681 gatcggggcg gccatcgtcc acggtgacta tcgcctgggc aacatgatcg tcgccgacgg
     4741 ccgtattctt gccgtcctcg actgggagct ctgcacgctg ggcgacccgc tggcggacgt
     4801 cggctacctt ctgaacaact ggattcagcc cgaggacggc gcgggtgagc tcgtgccgac
     4861 ggcggccggg gggttcccgt cccgggaggc cctctgtgac ggttacacgg cggcgacggg
     4921 gcgggacctc tcggggatca actactaccg ggctttttcc cactggcgtc tggccgccat
     4981 cggtcagggc gtctacaagc gatacctggt gggcgccatg ggcgagcgcc cggacatgga
     5041 cctggacacc tacaaggcga gcgtccagca gcgggcggcc gccgcgctcg agctgctgac
     5101 ggcttagccg ccgggcagca cgatctcgcc cagcggggtg tcgtcctggt cgatgacgcg
     5161 cagccggctg aagccctcac cttccagccc gatgacgatg cagggattgg tgatcacctg
     5221 agcgagcacg gcatccgcag gcggctggtt ccaggccagc cgtaccgtga ccgcatcgcc
     5281 tttcatggtc cccgcgacga gctcgaagcc gtaacccggc gtgggctgct ggcctctgga
     5341 gaccgcgacg agcagcaggt ccgtgggcgt ggtggcgggc tcgtcagggg cagacagcag
     5401 cctgcccccg cggatgcgcg ccacatcctc gatgctcacc acttccgttc cggcctggga
     5461 catcctgcat tggtcgtggt tgagcacttc caccaaagag acgtcgtcac gaccgctgca
     5521 gccgtaaagc gcggcgacga gcaacgcacc tagaattgtc cttgagcggg tcatctggga
     5581 gctcccccat ctgccttgcc cggcaagcgt acggcttcgg gtgcgacttg cccagagacg
     5641 tcggcgttcg ccgagaggtt ggtcaggttg acgcgaaact cggaggtgaa gtcgatgccg
     5701 gtcaggtctt cgccgtccac cgtgacgacg gccggagagt ccagagtccc gtaggtgccg
     5761 cacgcttctc ccgcgtcgca cagaaaggcg tcgtcgtcgg agtccgtccc ggcgaatatc
     5821 cggtagtcgc cgggcgcgac gccgtcgagg cggtaggcgt actccccgtt cgtggcctcc
     5881 aggacgttct gaggtgacgg aagggtctcc cccgccgcat cgacgagcac gacgtagtgc
     5941 acgccggcgt tggcgtccgg gctggtggtg aaaacccgca tcgttgcggt gacggtggcc
     6001 gtggggttca cggcatcggt cacgatttcg atggcgccgg tgtaggagcc cggggtaagg
     6061 cccgtgcggt ccacggtgac cgtgtaggaa cccatgccgc cgggcagcac cgagcttggc
     6121 gccacgtcca gccaggatgc ggctgtgccg atgctctgca ccgtcaccgt gccggagccg
     6181 acgttttcca gtcgaagaga ctgtgacgac agtgccgccc cgaaggtcag ggaactggct
     6241 gacacggcca gggtcgggcc aggcgggttg ctggccaggt ccagggcggc cagcaccgcc
     6301 ttctgggcgt tgatcaatcc ccagccgtag cggtcgtccc gccccggcgc gcccagatcg
     6361 tcggtaatcc ggcccgacgc gagcgcgttg tcgaactgga tgggggtcag gccggggtga
     6421 acggccttca tcagagcaac gacgccggca acgtgaggcg cggccatgga cgtcccctgc
     6481 agggatgcgt agccgaagcg caggctcgag gggtcgctgt cgtcggccat ggtgctgatg
     6541 accccgtctc cgatcccgtc gccgttgacg tcggtgacgt tggacccgcc cggtgccgcc
     6601 acgtcgacgg tggtcccgaa gttcgaatac gccgccggat tgttctcgat ggtggtggcg
     6661 ctgacggaga cgaccccgtc gtaagcggct ggatagctgg gcgaggcgga cgcatcgttg
     6721 ccggcgctgg caacgatgat ggtgcccgcc gcgcggatct cgtcgatggc ctgctgctcg
     6781 ctttcgagaa acccgccccc tccgagactc aggttgatca cgtccgcggc gcggtctgga
     6841 agggtgccgg agacgttgtc gaggcgggcg gcccatcgca tggactcgat gacgtcgaag
     6901 ctggtgccgc cgcccacacc gagaacccgc aggggcatga tcttcgcctc ccacgcgacg
     6961 ccggcaacgc ccgaggccgg ggtgaccgag gcggatgttt ccgccgccac ggttcccgca
     7021 acgtgggtgc cgtgaaagct gctggaaccg ccgaacgccc cgtcgcccgg atcggtcgga
     7081 tcgtcgtcgc gagcatcccc gtcggctgcc cgctgcagat tggcgatgaa gtcgaagccg
     7141 gcgatgacct tctcgtcgag gtcggggtga tccgcccgga tgcccgtgtc gaccaccgcg
     7201 acgatcacgt ccccatcgcc cgtggtgatg tcccacgcca gaggcaggtt gatggcctgg
     7261 tagtgccact gggaccggta gaaagagtcg cccggctccc ggtgtggctt ccgaatcagg
     7321 ttgggttccg caagctcgac gcggggatcg cggcccagcg cctcgatgac ggccagggtg
     7381 gcgtatcgat cctgctgatc ttcggtccac gaggcatcga atggtgcccg ccgatggatg
     7441 tgccggagcg cggccgttgc cgggccggct ttcagtagca tccgcgggcc ggactgagcg
     7501 agcatgctga ggccgtactc cgccgcgatc tcatcatcgg tcgtgcgcag gatgatctct
     7561 cgtggcagga acggatcgat catggtggtg gcccgctcgg ggacgaggtc ctgacccacc
     7621 gtgagcacgt agttgctggc gccggcgatg gggcgaacct cgatgaagta gtctccggcg
     7681 gcgggaacct cggcgacctg aacctctccc ggcccgccgt cggcgctgat cacgttctgc
     7741 tgcgagtcgt aaaggagcag ctcctgctcc gcgttctcgg gatcggcgat gttcaggtag
     7801 atgaactcgt tgccgaccat ggtcatggcg tagaaatcgc cgggatcgcc gtcctggaag
     7861 agctttcccc tttccggctc gtcgcccacg ccgacgcccg ccacgttggc gaaccccccg
     7921 agggtcaccg gattaggcaa aggctgcgcg ctggcaaagt cgttgttcgg cgccggcgtg
     7981 ctgcctacat cgttcacgtc gctgtcgatg gcgctggagg ccagaattcg tacggtaccg
     8041 ctgatgcttg cgacaggccc ctccaccgtg acggtgcgag tcgtgctccc ggtggcgccg
     8101 tcgtcatcgg taaccgtaag ttccacggtg taggtgccgg cctcctcgta ggtatgggac
     8161 gtgttaacgc cgctggtgct cgatgctcct ggaggctcgc cgaaggtcca tgcgtagctt
     8221 gcgatgcgtc cgtccgcatc gctggacccg ctggcatcga aatcgacgct taaaggcacg
     8281 gttccctcgg tcggggtggc cgtgaaggat gccgccggcg accggttagc gggggccggg
     8341 gtgggggtgt cgctgccgcc cccgcccccg ccacaggcag tcagggttaa tacgcaggta
     8401 accgtccaca cggtttttgg caatgcttgg cgaatcatct ttgcccactt ctgcccatca
     8461 cggccgggtc cgccgatttt tgcacagatc gggtgccggc tctgtgcgcc gggcagcacg
     8521 atggcgttgc ccgggcggtc aacgttgcga aaaggctggg gcgttgcgaa aaggctgggg
     8581 ccggaaacga cacaggcctc tgaaagaggc ctgtgcgtta ccgtgggtct gatccggccg
     8641 gctgccggct cagtccgtgt aaaaaaggcg tgtcagcagt gggcaccgct gaaacaactt
     8701 ctcttctggg ggaaggacac ttgaaaggga ggactgcatg aagttgtgtt gcacgcctga
     8761 actgttagtg gggctattat gtacagtaca aaatctgtgt caacccattt ttaataaaaa
     8821 tgtacaggtt ttgtttaggt cgttttgttt aggtcaatgt cgagcctcat gggatcagcc
     8881 cgcctcttga tccaccatca tctccagagc cagctccagc tgcgcgcgtg cctgggtggt
     8941 atcggtgtgc acgcgttggt agacctccgg ctcggcggcc tcatccgcgc agttcaacgt
     9001 gtactcggcg aaggcgttca gcccgctgat cagctcgacc agatgctgag ggcacgctgc
     9061 gggatccccg tgctcgctgg ccgagatggc gatgagctcc gcgtcgctga atcggcgcac
     9121 ggccgttcgg gcggcgcgta gcggcagccc ggccgtggtc gcgcaggcat gctcgatctc
     9181 ctgccagcct gccggccagg tgagggtggg tatgccgagc tcctggacgg cggcgatctg
     9241 ggccggcgtt gcgaactggt agatggcgac gatccggctt tcagggtgga aacgcgccag
     9301 attgttgatg tggtcgacga gcagcacggg cagctgcgca acgatcacgt cgatccgctc
     9361 ggtgccggtc tggtcgctca tgaaagcgtc catgtttgcc cagcgggcgc ggacttccag
     9421 gcggttgtcc gagccctgct gttgctccag cacgaggagg ttcggcccga tcaggccgac
     9481 cgtggccgat tgcagggcct gaagccgggg ggtggcagcg gaaagtcttt cgtcaagttg
     9541 ctcgtcggaa agcggcgcaa ggctgctgat tgggtgcccc tggtcgatca gacgtttgat
     9601 gcgctgaagg gtctgaagct gctgcttgct gtagaagcgc gtcttgcccg agcggtaggt
     9661 gggggccagc ccgtaacgcc gctcccaggc acgcaggcgc tccaccgaga tgccggtgag
     9721 cgtggcgacc gtgcctatcc tgtagaggtc gtcgtcgatg gcggagcgtg ataccaccct
     9781 gcagcgtacc agtacaatac ctgtacagaa ttgtacatgc taacgtcatt ttgtccaggt
     9841 agccggtctg acgccggtgc agggagtgca gagagtgcag gaggaaggtg gcgtggcgac
     9901 agcgcattgt ggcgctgtct tcacggtgag ggaggaaggg gagagagaca ccgcgccacg
     9961 ccgatttcat ttgcagatcc gacgagcggg tccgcaaaga aggcgcgcat tctacggatc
    10021 tgcaggcagt gatcaagcgc gtccccgggt tttttgttac gaatcttttc acctgggcgg
    10081 agactgcgac cagggtgttc acacggacga tctggttcgg gagaatgggc tcgagaggcg
    10141 gtcctgtgag ttcctgcagg gggccttgcg ctttgatggg ttactcgcaa ggggaggcaa
    10201 cggtggagaa caatctcggt ctttttctga cgaagcgggc gtttctgacg ccccagcgtg
    10261 aagggtacgt cgacagccat tcgggcctgc gtctgacgtt tcggcagctg aacgaacgat
    10321 ccaaccagct cgccaattcg ctcctgcagg cgggcgttca gaaggaggaa cgcgtgggtc
    10381 tcctgctcat gaacagcgcg gagttcatgg aggcctattt tgcgctggcc aagatagggg
    10441 cggtggtggt tcccctcaat tggcgtcttg tcgccgacga gctggagttc atcctcaagg
    10501 acagcggcac ggcgcggctg atcttcgacg aggatttcgt cgacaccgtc gccgagctcc
    10561 acgcccgcgg cgaccggacc gacatccggc actggctgca ggtggagggt tcccaggagg
    10621 ttgcccactt cgctgagagc tacgaggctt tccgggacaa cggggcggcc gaggagccgc
    10681 cctgcacggc ctgtgacgac gacatgctct acatcatgta cacgtccgga accaccggtc
    10741 tgccgaaagg ggtcgtccac actcacacca cggccatctg ggcgatcctc accatcgcgg
    10801 cgaacgcgta ctacgaggaa accgaccgct atctcggggc gctgccgatg tttcacgtcg
    10861 gcgcgctgac acctctggcc gtcaacgtct atcgcggggc cacttcggtt gtgatgcgcg
    10921 cgttcgatcc cgtgctcgcc tgggagctca tcgatagcga gaggatcacc tcgggattga
    10981 tggttcccgc catgctgaac ttcatgctcc aggtcggaga ggcgaaggac cgctacgact
    11041 actcggctct gcgctggatc atgaccggcg ccgcgccggt acccgtcgcc ctgaccgagc
    11101 agtacgtgca gatggggatc agcatcctgc aggtctatgg cctcaccgaa tcctgcggcc
    11161 cggcgtgcct gatggattca gagaacgcgc tgcgcaagcc cgactccacg ggtaaggcct
    11221 tcttccacac ggatgtgcgg gtggtcgacg cccacggcaa cgactgcgcg ccgggcgagg
    11281 ccggcgaagt ggtggtgcgg ggcaagcaca tcatgcgtga gtactggaac cggccggacg
    11341 ccaccacgga gactatcgtg gacggctggc tgcacaccgg cgatgttgcc gtcatggatg
    11401 aagaggggtt cgtttccatc caggatcgga tcaaggacat gatcatctcg ggcggggaga
    11461 acgtttaccc cgccgagatc gagggcgtgc tgatcagcca cccgaaaatc tccgaggcgg
    11521 ccgtgatcgg tcagccgagc gagcgttggg gcgaatctcc cctggccatc gtggtaaagg
    11581 ctgatcccga tctggacgtt gccgaggtga tggagttctg tcgcggcaag cttgcagggt
    11641 acaaacagcc aaaaggggtg gagttcgtcg acgagattcc gcgcaatccc agcggcaaga
    11701 tcctcaagcg catcctgcgg gagcagtttc ccggtcccgc gcctgcttga ccgtctgtcg
    11761 aggccgcggg tggcgggtcc tccgcagggg tcggatcaga tccagtagcc gacgaagcgt
    11821 tcgagggcca cgaggcccca tatcagaggc accaggaaga tcgccagcgc gacggcggcg
    11881 gaagccatgt ccttggcctg gccggccaga tcgttgtgct cgtgacctat cctgtccacc
    11941 accgcttcaa ccgccgagtt cagcagctcc gcgatcagca ccagcacgca gaccgtgatc
    12001 agcacggcca gctgcatcca cgtctccgcc agcaggacgg cgcagggcag cagaaccagg
    12061 gccacggtgc attcctggcg aaaggcggac tcgtgttgcc aggcggcgcg gatgccctga
    12121 gacgagtagc ccgctgcctt tctgatccgc ttgatcccgg tgtagcctgg tttggtcatc
    12181 ctagccctcg agcctaatca tcgtccgggc aaggcccgtg ttcaacctga atggtcgagt
    12241 gatcgatgcc gaaatcgtcg ttgagcacga ccttcatgcg agacaccacc ccggcgagct
    12301 cggtcccctc gtcgacgctc gcatgcagcg tgaccagggg cttctcggag gtcagtgccc
    12361 acgcgtgcac gtggtgaacc tcgatcactc cgggtacccg cgcagcgagg gtctcggcga
    12421 tctccgtgag gttgatgcca tgcggcacac cttccagaag aatgtgggcc gattccttca
    12481 gcacccgcca ggcgccccgg ccgaggatga cgacgatgac gacggcgagc agcggatcgg
    12541 cgtagagcca accggtcaga tagacggtca gcgcggctat gatggcggcc gccgagccga
    12601 gcaggtcgcc gatgacgtgg agcgcggcgc tgcgcacggt ggcgttgtcc tcggccccgt
    12661 gcagccagcg gaaggcgatc aggttgacca ccatgccgac gacggcaacg gccagcgcag
    12721 gcagagccag cattccttca ggcgcgcgca gccgggcgta ggcctcaacg agtatccaca
    12781 ccacgagcgc catcagggtg agcccgttga tgaatgcggc cagcacctga aagcggtggt
    12841 agccgtagga gagcttgtga tcgttggagc gtttcgacaa ccggagcgcg taccaggaca
    12901 ggcccagcgc ggtggcgtcg aggaacatgt gccccgcatc ggcgagcagg gtcagggagt
    12961 tggccagcag cccaccgatg acctcgacga ccatgaagcc ggcgatgagc acgaaagcgc
    13021 ggccaacgat gacctcgttg tccctggtat gaatgtggtt gtggtcgtgg cccacgttta
    13081 cgcgttgtcc tgcagccgac tcacgaacgc ccgtgattat accgactcac cgcgctcggt
    13141 ggtccgctcc gtcccgggcg tcgtgggtca cgtcgccacg ggactcggct cagagcagcg
    13201 tcttcggatc gatgcccagg gcgaccttgc cgaccacctc gtagataggc tgcgctacca
    13261 tcagatgctg cgtggcgacg tgcacgtcgc gaaaacaccg ctgcagcgcg ctgtgcgcgt
    13321 aaatcgacga tcctcccgcg gcatggtaga ggaggtccac cgcctcggcc gccgaccagg
    13381 cgttgttggt ggcggccagc ctgaggcggg ccttggtttc caggccgagg gatccttcgc
    13441 ggtgggcttc cccccaggcg tcttcgatgg cctcgcgggt gagcgccgcc gctgactgga
    13501 cggcggccat cgctctggcc aggtcgcgct gggccccaga ccgggcggaa agaggccggg
    13561 cgctgccggt ggggaccttg tcccgggcga gcgtggtgaa agcctcgatg gcgtgctctg
    13621 cgatgcccag tgcaaccgcg gacacgccaa gggcgagcaa gcccagcgta ggaaatcggt
    13681 agaggggcgc gtccacccgc gcgcgcttgc ccatggtgac ccatcgcccc gcgggaacct
    13741 cggcgtcctt tacctcgatg tcgtggctgc cggtcccgca taagcccgac gtgtgccagg
    13801 tatcgtgaat cgtcacgtcg tctgcgggaa agaaaaccag ggcgatctcg ggcgagccga
    13861 accggttctt gcgcggctcg ccatcttcca tgagctggca gccgccgcag atccaatccg
    13921 agacctcgca cccgctgccg aagggccaac ggccgctgac cctgagaccg tcgccttcgg
    13981 caacggcgat gcccagcggg gcggtgacgc cggaagtgat ggtatcgggg ttgcaaccgt
    14041 agatcgtgcg agaccattcg tcgggcagcg aggcggccat catcccggtg gtcacgccga
    14101 tcatctggca ccagccgacc gccccgtcgg cgcgggccgt ctccttgagg gtttcgaaga
    14161 aagctagagg ctcgacctgc aggccgccgt agagctccgg gacgagcagg cggaacagtc
    14221 ccgcaccagc catgcgattt gcgaggtcgc cgggcatccg gcgatgctgc tcgatctccg
    14281 gagcacggcg gcggatttcc gcagcgaagc ctgcggcggt ctcgaccggg ttcattgggc
    14341 caggcggaac ttcatctggt aagcggcctc cttgccgtcg tagtagccgt tgatgagggc
    14401 ttcctcggtg tagcccattc gaccatagaa acgacgcgca gacacgttgc ccgctctgac
    14461 ctgcagctcg atgtgctggc agccggccac gcccgcgctc ttgtgcagcc actcgacgag
    14521 gtttccaccg atgccgcgcc gtctcaaatt cgggtgcacg gcaagcaggt tcaatgcggc
    14581 cttgtcctcg ccgaaatcca tcgaggcaaa accgccgatg aagcgaacgc cgccgagctc
    14641 cacctccgcg acgatgacgg ccgtttcggg atgccggatc ttggcgcgga tctgcggcgc
    14701 gcgccagcgc cagcggagcc cgtgctcgat gtagtcccgg gacatgcgcg cgatcagccc
    14761 cgcgtctctg attgcggcca gcttgacatc gcagactttc aattcggcca ttgaccgatt
    14821 attgccaatc ccccatcccg aggggaacgg gtccgggcgc cgaggttgca aacgacttgt
    14881 agaaccggtc agaaggcagc cgggtcgcgg aacgccagat gcaggctgcg cgcctcgaac
    14941 cgccatccgt ccggcccgcg ggcgaaccgg tcctggtagt ggccgacggc ggcgggaagc
    15001 gtggtcggcg cgggttcgct tccgggaccc ggtatcgacg gtttgtcggc aatgtggcga
    15061 tagagggtca gataagcgat gccgcgggca gtgttttcat cgacgacgtc tacgaacacg
    15121 ttggttatga cgtgacgcga gcgcagctcg tctggccgcg ccgagatggc ggcgtgaatc
    15181 tcctcgcgcc cgcgcagcgg gcggccgagg tcgagcacgg cgtcgtcggt gaaaagggag
    15241 acgaaatggt cgtagtcccg gaagtcgatg gcgcgggcgt atgccaggga gagcgcttcg
    15301 cagtcgcgct ggatttccgc gatcagctga ttgtccacgt cgggtttctc cctcctggtt
    15361 tgagcctggc catcgggttg gttatcgttc ccgatccgtc gcagatcgac aagtcggtcg
    15421 cgctttcaga ttgggggaac caatggaacg aatgtcgctg gagttcgtgg gcaaggtggc
    15481 cgtgctcaaa ttcaatcatc ccgaggtaat gaatgcggtc ggcagccaga tgctgctgga
    15541 tttcgccgac gcgttgaagg acatcgctca tccgggcaat ggcgcccgct gcctgctgct
    15601 caccggcgag gggcgcggct tctgcgccgg cgccaacctt caggatggcg agcgctccga
    15661 gcgccgcggc ggcgccggtg acggcctgcg tgccagctat catccgatac ttctcacctt
    15721 gcgggatctc aacatgccga tggtcaccgc cgtcaacggg gccgctgccg gcgtcggcat
    15781 gagctttgcc atgatgggcg atatcgtctg cgcatcttcg aacgccttct ttctgcaggc
    15841 gttcgcgcga atcggtctgg tgcccgacgg tggctcgacc ttcctgctgc cccgcctggt
    15901 gggatggggc cgcgccatgg agctctccat gctggccgag cggctgccgg cggagaaagc
    15961 ccacgagtgg gggctggtga accgtttgtt cgacgacaac gcggccctgc tcgaaggtgc
    16021 catggccatc gccgaccggt tggccagcgg gccgagatcg cttgccctga ttcgccaggc
    16081 ttactggaac acgtggcaca acgcgtttga gcagcagctc gatctcgaag ccagactgca
    16141 gaatcaggcc ggcgcgtcga gagacttcgc cgagggcgtg gccgcgttct tagagaagcg
    16201 cgacgcggag ttcaccggcg agtaacgact acgcggcgag cagctcctcg atgtgcgcat
    16261 gagccgatgc ggccagcgcg gcgaggtcgt aaccaccttc taaagcggag acgatgcgtc
    16321 cgttggcatg ggtcttcgcg ctggcgacga tgagccgggt gatccagcgg aaatcgtcct
    16381 cgcacagctc gagctgagcc agggggtcga ggcggtgggc gtcgaacccc gctgagacca
    16441 ttatcagctg gggctggtgg ttgtcgatgg cgggaagcca ctgcgcctcc acggcccggc
    16501 gaaagtcggc gcctgcggtg cctgcgggca ggggcgtgtt gacgatgttg gggcgcgcca
    16561 ggtctgcgta gcggtggggg tagaacgggt gctggaagct cgagcagacc agcacctcgg
    16621 gccggtcctt gaatatgtcc accgtgccgt taccgtggtg gacgtcgaag tccaggacgg
    16681 cgacgcgctc tacgcggggg tcttcgaggg cggtgacggc ccccagggcg atgctgttgt
    16741 agaagcagaa acccatggcc gcgtcgtgct cggcgtgatg acctggaggg cgaacggcac
    16801 agaacactgt ggattggtct ccctcgagga cggcgcgaac gccgtcggca accgcgccgg
    16861 cggccagggc ggccgcccgc aggctggatg gccccatggc cgtgtccgga tcaacgggca
    16921 gcagtccgga ctcgggcacc gattcggaca gcgtgcgcag gtaatccgga tcgtgcacgg
    16981 ctcgcagcgc ttcctggctc gccggcaagg cctctgcatg cttcaggtcc cgggacagac
    17041 cgctggactc gagcagttgc agcaccgcgc gaagacgatc gggtcgctcg ggatgccggt
    17101 gctgcatctc gtgcagcagg cagtcccggt gagtgatcag cagcatcggc cgatggtagc
    17161 agggtccccg gaaggaagca gcggggtcag tttaagtcgg cgaggcgatt catcaggtgc
    17221 tggattccgg ttaccggtac cggacgagag acgagcagcc cctgggcgtc cgtgcagccc
    17281 cactcggcga gcaatttgag ctgctcctcg gtctcgacgc caaccccgac gacgcgcagg
    17341 ttgaatcgcg cccccaactc gcagatctgc gcggcgagat cggccgcctg ggtatcctcg
    17401 gacaacgcgc tcaccacggc tcggtcgatc ttgagcgtgt ccaccgggca gcgcagaagt
    17461 ttctcgggct cgatgagccc gccgccgaga ttgtcgatgg caaagcggac gccccagtcg
    17521 cgcagggtat acatctgctc gcggtagtcg tccaccagtc gcagcatgtt gcgttgcgac
    17581 agctccagct ccagggacgc ggggttgagc cggtgctcgg cgctgttgcg ggcgatctgg
    17641 gtgacgatgc tgcggccgtg aaactgctgg ggcgatacgt ttaccgtcag cagaatgggc
    17701 tgcaccatgc ggggaagcca cgcggcggtc tcgcggcagg cgcgctctaa aacccagtca
    17761 cccacccggg ccatctgtcc ctgctgctcg accaggggaa tgaagtggct gggggcaagc
    17821 ccgccgatgt cgcggccctg ccagcgcagc agcgcctcaa cggcggtcag ccggttgtcc
    17881 tgcagactcc actggggctg cagcagcagc gagaactcgt cccgctccag ggcggaaggg
    17941 atgcgttcgg agatcgcggt gtcggggttt gcgccgagga cccggtcccc gccagcctga
    18001 gaggcagcgt tggcggcagc ccaggcgctg gcccacagat gatcggcgcc gttgaaggag
    18061 ctggcgaagc tgcacccgat gctgatcgtc agatggatct ccccgcccgc gtcgatgagc
    18121 ggtgcgctca ccgccaggcg aagatcgttg cccagccgct cgagctctga cgactggatt
    18181 ctgggcaggg tgaccacgaa gcaggtggcg tcgtagaacc cgagtcgggc ctgagtgggc
    18241 agctttgact ccagcctctg gctgagcgcg gcgagaaggt ccgtcttcgc gaccggatcc
    18301 aggctcccat agagatgctc cgcatgatcg agcttgacca ccatcatccc cacgggctcg
    18361 tcgacatgcc tcgacagcgc atcgaacagc tcctcgtgtc gcggcaggct ggtgatcggc
    18421 tcgttgcgat cgatggcggc gaatcgggcc cggaagcgtc gggcattcac gcagttgttg
    18481 atgtgctgga gcaaggcctg gtcatccaga gcaggatcga acgcgtccgt gccgggctcg
    18541 cctctgtcgg gccgcaggta aatcctggtc gtgggcagcg cgccgactcc ggtcagattg
    18601 tccggctcgt cgcttacgat gacgtccggc gttgccttgc cgctgatcag ggttacgaga
    18661 aggtcgcgat cgtcagccgc gaagaggacg cgccagccgg cgcgggctcc cagccgccgc
    18721 aaccggtctc gcagctgctc gtccttgatc agggcgcaca cgatcacgat cgacctctta
    18781 aaactcacgc ggaaaatgcc cgcacaagtg ccgaagctag ccgatcatca ggattaattg
    18841 cttgtggtcg gtcacagcgt gaaagaaacg ccccaacggt tccaggtagg ggctcgagcg
    18901 ggcgggaacg tcgttgatga gctctacctc cacccgcctg cggcgccgga cgagatgggc
    18961 caggggctcg cagacccgac cgaggcaggc atcgtccggc ccgcggtgga attgcaggcg
    19021 cttgccggca ttttcgatca ccagggccag ggatccctcg tgaaaagcaa gataattgtt
    19081 cggtcggcgg gccggcaggt cgggtatggc ggtgacgagg gcgcagggcg aggccggatc
    19141 gacggcgctg atccagaaat cctccggcgt ctgccagccc tgctgcagac gccgcagcgc
    19201 gccgggtgac acgaactggg gtccagacag gccggtgaaa aagtagccgg ccgtcacctc
    19261 gcctgccagt tccatgaggc gcagtgccct gaagacacat cgccaccgca gcagacgccc
    19321 ttcgcgattg gcgagctccc ggcagatgat gccgtatcgg tcgagcagca tgcgggcgcg
    19381 gtccttggcg tcctccagct cggtgagcgg atcgatgtca gcgtcgaacg ggggcttcac
    19441 cgaccagttt cccgtccagg caggcgccgg cctggcgcgc cgtcgtccct gccgatggct
    19501 ggaggcccgc tgcggcgccg cggcgttcaa ggcgtagccg cgttcgagtc cctgccgtag
    19561 cggggcgagg gtgtccgagg tgatctcgcc ttcccagacc gacgcccacc attgctcgtt
    19621 gaatgcttct ccagaaagtc cgcttctgtc ggccagctgc acaaacgggt atcgcgcggt
    19681 cggatccgtg aagagcttgg ccagggttga tggctcatcg acgccgtcgt cgatgagctg
    19741 cagatcctcg ggatagccga tggctatcgc cttgcgcccg gctccgcgcc actgcatgcc
    19801 gtgacgcagc agcacgtcgt cgagctggga atcggccatt gccggaatgc gggcggaaac
    19861 gacgtcttcg agccacgccc gcacgggggc gcggaacccg cgcaagggct ccatgacctc
    19921 gagaacgtgc tcatccccgc cgctcacgcc gaagcgctgc cagcttgcca ggaaaccggg
    19981 cagggtatgg aagggccggg gctgcagttc cggcctggct gccgcgcgct gaaaccgcag
    20041 cagggccgcg tagttttccg catcgcagta gtacagcccc gggtcgtctg ccaccagggc
    20101 gccgctcacg aggacctcgt tctcatcgag caggctctcg ggaacccggg gcagaatcca
    20161 ggcgatgtcg gactccgtca aggggccgta gaaagacagg atctccaggg cgagctgctc
    20221 ggcacttcgg ctctcctcga cgtccgggac cgtcccttgg aaggcaaggc cgtcccgcag
    20281 accccgttgg gcgagcccgt ggagcaactc ccgatgggtg agccaggcgc ggttctctcc
    20341 ggagccggcg cgctcctcca gccagacgag gtgctcgtgc tcgggtaccg gcgcgtccgc
    20401 actggtggcg gcccggacct cggcggccgg aatcagaacc cgctccttga cccattcgga
    20461 ccagtcctcg gcgctcaccg ggcggtatcc ttcggccgtg cgctgccgtt tcgccaggta
    20521 ctcctgaatg atttcgcgcc ggatgggcgg gcgcaggccg gcgttgcgca cggcgctgcg
    20581 gatcagatcg tcggacagcg cgctgggccc ggcgtcttct tccggggtgt cgtcggcgta
    20641 catgtagcgg ttgatctggg cgaaggtgac gttggccgcg aaggggctgg gcgagctggt
    20701 ggacacgaac gtccactcga tggtgccgtc ctccagttcg gtcagcatct gtcgaagcga
    20761 gtcgagttcg aactcgtcct tcaggcaggt gcgccagctt tcgagaagca tggggaagtc
    20821 ggggtagccg gccgtcgcgg acatcagctt ctttgcctgc agccggctca tccacagcgg
    20881 caggcgctga ttgaagcgct gcttgctcaa cagcagcgcc cggccggcgc agtcacgaaa
    20941 acgcgcgccg aaaaagccgc tttgctcgag gc
//
LOCUS       DBNHLM010000278        12152 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1177915, whole genome shotgun sequence.
ACCESSION   DBNHLM010000278 DBNHLM010000000
VERSION     DBNHLM010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12152
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1177915"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..1049)
                     /locus_tag="AC16QF_12890"
     CDS             complement(219..1049)
                     /locus_tag="AC16QF_12890"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR6449504.1"
                     /translation="MRKFLGRMTRRGIVSLLAGLLVTLPIGVNAAKDQSPVLAQIAKW
                     GKIRVGMSGSQPPYNFVSREGKLIGMDVDIAELLAKSLDVQLEMVQMNFNELLPALES
                     GKVDLVISGMTATLQRNMRAAFVGPYHITGKSILTRSETIAALGSNDLNREDLRIAAL
                     KGSTSEDFVRRVAPNAKLVATDTYDEAIDQLMANKAELFVADAPRVMLEVMRWPDAGL
                     TAAQRPLTIEPIGIAVPASDPLLLNLVENYMAALESSGALEKLEEKWFKSGGWLVQLP
                     "
     gene            complement(1170..2186)
                     /locus_tag="AC16QF_12895"
     CDS             complement(1170..2186)
                     /locus_tag="AC16QF_12895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HIR6449505.1"
                     /translation="MQAIRCHEISEDIGAVRLETISLDPPGEGEVQVRLKACAVNFPD
                     LLMIQGKYQFKPPLPFSPGTEAAGDVVAVGDGVSAFDVGDRVVVALRYGGFAEAVNVP
                     AESVQPVPGSMSYDKAASYRTAYLTAYVALAKRGDLTPGEVLLVHGATGGVGMAAVDL
                     GKRLGAVVIATGGSDEKLAVVKARGADHVINYTQADGTLGGFREEVKALTDGRGADVI
                     YDPVGGDVFDESMRCINWGGRILTIGFTSGRWPQAPVNLILIKQIAVIGVRAGEYGRR
                     DPAKGREAERALYAMAAAGEIDPYVCAGFPLERAVEAMRMLEQRRVVGKVVVTMNGFD
                     VASP"
     gene            complement(2196..2591)
                     /locus_tag="AC16QF_12900"
     CDS             complement(2196..2591)
                     /locus_tag="AC16QF_12900"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449506.1"
                     /translation="MIRDAETFEHLLAAVRRFVAEVAVPNEERVEADDEIPAEGVEAM
                     RNLGTFGRSIPEAYGGSGLTSEELALAFMELTRCSVAYRVVQIFGGQGYVKGRGSERF
                     YRDVRAFRIYEGTSQIHLLNIAKQLLKDA"
     gene            complement(2591..3535)
                     /locus_tag="AC16QF_12905"
     CDS             complement(2591..3535)
                     /locus_tag="AC16QF_12905"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6449507.1"
                     /translation="MEIYLHHYPASLFSEKIRLLLGYLGGSWHSVEIPSIMPRPLLMP
                     LSGGYRRTPVLQIGANVYCDTAVIARGLARHFGDEDLFAAGFTANRVAEWADTQLFRV
                     TVALNFRPEAVGGFMSQLSPEEIAAFQKDRAELVGDAPMVSFSPEAAQAYLATYLDQA
                     DRALDGGFLFGGKPCIADFCFYHCLWFLENNPLNAPLLDAYGNVRGWMQTMAGFGHGS
                     VAPSSGEAALEHARSCSPVAPVLESPLAAPFELDVGRRVTVTPADYGRVPVAGSLVAW
                     DCEEVVILREDAATGPIMNHFPMAGFQVAPAGNLGSSG"
     gene            complement(3620..4945)
                     /locus_tag="AC16QF_12910"
     CDS             complement(3620..4945)
                     /locus_tag="AC16QF_12910"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR6449508.1"
                     /translation="MMRDTSLTGLAESLVAYGTRRGADQVEVAIRTGSNFSASIREGE
                     LETLVEAGFRELGVKLIVDQKVATEASSDLSEATLKRLIDNGIGRARLSGADPFAGLP
                     EKEPVKVRAEALDIFDEAVVDMSPESKIAAARRVEAICLGDKRIRRSMGAGFGTTIET
                     LYLANSNGFSDAFRQTACSNGVYLQVGEPDNLISEGKYDYSRNLEGLRSPEDIAAEAI
                     DRSTRLIGARKVPTQNVPVVLEPEMSAELLQFLASCVDGRGVYRKQSFLADRIGERVA
                     GANVNVWDDGLMPGAPGAKPFDREGVPLRKTAVIEKGVLKSFLLDSYSARKLGMKPTG
                     NGAGPNNLYLASGKDSPADIIGSVDEGLLLTGSIGFGAVPTTGDISRGAFGIWIEGGE
                     LAYPVAGITIGANLGTLLQQVEMIGDDLDHRRYVNGPTIKVAEMTIGGT"
     gene            complement(4942..6465)
                     /locus_tag="AC16QF_12915"
     CDS             complement(4942..6465)
                     /locus_tag="AC16QF_12915"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR6449509.1"
                     /translation="MRADRRDFLKKTGSAVAAASTASLWPDLLLAEEVPTTAEALFET
                     RFGVSRGDMGRLLSLALGKGGEFAELFFEYKIANGVTMEEDIVKESSERVSLGVGIRV
                     LKGEQTGYGYTSDLTFEGMSQAARTAAAIASEGGRVEIAPLRLRANALQVYDLERPFA
                     ESSLSSRMILLRQTYDAAQAYHPKIEKVRVSLRDEIQYVLVANSEGLLASDTRPQVRL
                     IASVTAADGGNRSTGLSTAGGRVGSAFFSSARTPKELGREAAEEAIVLLGAVSPRPGD
                     QTVVLDSGQSGVMIHEAVGHPLEADSNRKKRSVFWDKLNQTVANPLVTIYDDPTIAHF
                     RGSHNMDDEGTLPRKTMLIEKGKLVGYLQDRLSAKLMKMSPSGHGRRDGFENVPLPRM
                     ANTNLARGESDPEQIVRSVKRGFFAKTYQGGMVQPSGKFTFSVNMGYLIEDGKLTAPV
                     KNATLIGSILDVLPEVTMVGNDMGTFLGTCGKGGQSAPVTAGTPTLKINSMTVGGRS"
     gene            complement(6534..8207)
                     /locus_tag="AC16QF_12920"
     CDS             complement(6534..8207)
                     /locus_tag="AC16QF_12920"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR6449510.1"
                     /translation="MARPLIRPGIMLRVLRFSLPVAVAVLAFAVPLRVGAEVVAFELR
                     EFFDDYYSSGAMAVQGQALLTERVLAEFYARRGYRPAWGDPALVAELFDLLARSVDDG
                     LDPDDYNLTRLRALRERAASSPKAVPERDLELLYTSSLLRFAYHQRFGKVNPKTLVST
                     WNFGRQLAPGVDAADTMNQIVESGSLREGLAARVPRGPLYRNIQMALARYRSAAAAGG
                     WEPVPEGVTLTIGMRDERVVSVRRRLAVTGDLETGAVSDPELFDADLESGVRRFQHRH
                     SLSVDGAVGPATVRAMNVSVEERIDQLRLSLERARWIMTARDTSVVVVNIAAAEVYVL
                     EEGEMVWQRRAMVGRTYRQTPVFRGLMTYLEINPTWTVPPGILRADILPKVREDPGYL
                     ARSNMTLLDRSGRAVDPYAVDWQALEGMPYIFRQEPGPTNALGLIKFMFPNEHFVFIH
                     DTPNRELFERAERTFSSGCIRVEDPFSLAALLLRDPANWDEAAVRAAVETGETRRVRL
                     ADPWPVYILYWTAQVDDDGVVRFYPDVYQRDVELLGELDGDVRIDLPAS"
     gene            8351..9274
                     /locus_tag="AC16QF_12925"
     CDS             8351..9274
                     /locus_tag="AC16QF_12925"
                     /inference="COORDINATES: protein motif:HMM:NF019896.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HIR6449511.1"
                     /translation="MKWLICLLVAGFSLPAAAYREATVPFGVKVQGIENRFAEFGVYA
                     RPGEILALDLIDVAPEEIGATFDSAPVKTGHGRINLKMPGAPGTHRVELTDQRSGSRM
                     RLNVFVMWQHDGARDDVLNGYRIGQYPRDPFRGLAVYRPPEAFVEVTPDNAHTRVSPN
                     FTLGQFVCKQGSGFPKYVVLRPQLVQKLELVLGELNAVNPDVTGLTVMSGYRTPAYNR
                     SLGNGRHSRHLWGGAADVYVDANGDGIMDDLNGDGRRDRGDAEWLATFIDRLEHAGRF
                     GEHIGGIGTYNSNAHHGPFVHVDARGYRARW"
     gene            complement(9375..10328)
                     /locus_tag="AC16QF_12930"
     CDS             complement(9375..10328)
                     /locus_tag="AC16QF_12930"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6449512.1"
                     /translation="MEFREIENGEIVLRVALSGHGPVILCVHGWPELWYSWRHQLRHF
                     AERGYTVAAMDVRGYGGSSKPHAIEAYRMTELCADVAAVARELSDEPVVLFGHDWGAP
                     IVWNTALRHPESVRAVAGLSVPYLPAGDVGTLDLFRGIYAGRFFYQLYFQEEGVVEAE
                     VEGDFETALRKIYFSLSGDAPLDDWLKNKPADASLLDGMVDPRPFPEWMSEEDLGIYV
                     SAFTAGGFRGPINRYRAQNLDVAESGPLRGLPVEQPACFVGGERDAVRRFVPGMDLYA
                     DPGAACADFRGSTIIAGAGHWVQQEAPEATNEALQRFLDGL"
     gene            complement(10426..11208)
                     /locus_tag="AC16QF_12935"
     CDS             complement(10426..11208)
                     /locus_tag="AC16QF_12935"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HIR6449513.1"
                     /translation="MRHRLLGLADHRSPFPGCGRAELLRAALSWGRRVRNRRESVLND
                     RLTIDYFSDVLCVWAWIAQRRVDEMNDQWGETVRLGFHCVDVFGDAGAKVAAGWSARG
                     GYEGFAQHVREVAIRYEAPAVHEYLWTKVRPVTSGTAHLVLKATEIAESPLEMTRLSR
                     ALREAFFVDARDVGDLGVCLEVAQGLGLAAAELKRHVDSGAAMAALLADYRKAEERGI
                     RGSPSWVMNEGRQVLYGNVGYRILNANIEELLRHPEQEASWC"
     gene            11224..>12152
                     /locus_tag="AC16QF_12940"
     CDS             11224..>12152
                     /locus_tag="AC16QF_12940"
                     /inference="COORDINATES: protein motif:HMM:NF021254.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HIR6449514.1"
                     /translation="MRLAINQAFSYLLTMQSGIPLSGKAPNLFGLQLALVSALLVSEM
                     FFISYRYDAWTLLQTTDSPWLTWLAYGGYFLKSAIAFVAALLVTIGTRLKAHYDDLVT
                     VTAPRSPWALLLAQLAAYAVLVWLTHLIFADPAEGAPPGAIIVGAWVLATTATVCIWL
                     SLIAPFSYWRRLVRQERTPIAASVLVASVALVIGASSQQLWGPLSQMTFASVEWVLWL
                     IDDEVYVDRERMILGLPYFQSRIAEPCSGYEGIGLVTVFTTLYLAIYRDHFKFPNALL
                     LFPIGIIAIWCANVLRIVALIVIGNDFSPSIAI"
BASE COUNT         2192 a         4174 c         3679 g         2107 t
ORIGIN      
        1 cctggctggt cctgcccgat cttaacaaaa gccgcccgag ccgagccccg gctccgcggt
       61 ttcagccgtc ttcgctttgg gtcattgaca tcccgacgag cgcaaggatg acgggcacaa
      121 cgaggatcag gttgggctga cggggcgagg atagggacat ggtccaggct cgcaccgaag
      181 gaatcggcgc gaaaccagga ccacgggttc atctcaggtc acggcagctg aacgagccaa
      241 ccgccgctct tgaaccactt ttcttcgagc ttctccaagg cgccgctcga ctcgagagcg
      301 gccatgtagt tctccaccag gttcagcagc aacgggtcgc tggcgggaac cgcgatgccg
      361 atgggctcga tggtcagcgg acgctgggcc gcggtcaggc ccgcatccgg ccaccgcatg
      421 acctcgagca tgactcgggg agcatcggcg acgaacagct ccgccttgtt ggccatcagc
      481 tggtcgatgg cctcgtcgta agtgtccgtg gccaccaact tggcattcgg agccacccgc
      541 cgcacgaaat cctcgctggt cgagcctttc agcgcggcga tgcgcaggtc ctcgcggttc
      601 agatcgtttg agcccagggc ggcaatggtt tccgagcgcg tcagaatgga cttgccggtg
      661 atgtggtaag gcccgacaaa cgcggcgcgc atgttgcgct gcaacgtggc cgtcataccg
      721 gatatcacca gatcgacctt gccggactcc aatgccggca gaagctcgtt gaagttcatc
      781 tgcaccatct cgagctgaac atcgagcgac ttggccagca gctcggcgat gtccacgtcc
      841 atgccgatga gcttgccctc ccggctgacg aagttgtacg gcggctggct accggacatg
      901 ccgacgcgga tcttgcccca cttggcgatc tgagcgagaa cgggcgactg atccttggcg
      961 gcattgacgc cgatggggag agtgaccagg aggccggcga gcagactgac aatgcccctt
     1021 ctggtcatac gtccgagaaa cttacgcatg ggtcttcctt agttgattgg tttccatatt
     1081 tcttgacgcc cgccttcaaa cgggcgggtc gaacggggag gccgttatac aacaagccag
     1141 gcgcgacgaa cagcctctcg gcggaggagc tatggtgagg cgacatcaaa gccgttcatg
     1201 gtgaccacca ccttgccaac caccctgcgc tgctcgagca ttcgcatggc ctctaccgcc
     1261 cgctccagcg gaaaacccgc gcagacgtac gggtcgatct cgccggcggc cgccatggca
     1321 tacagtgccc gctcggcctc gcgccccttc gcgggatccc gccgcccgta ctcgccggcg
     1381 cgaacgccga tcacggcaat ctgcttgatc agaatcagat taacgggcgc ctggggccag
     1441 cgcccgctgg tgaacccgat ggtaaggatg cggccgcccc aattgatgca gcgcatggat
     1501 tcatcgaaca cgtcgccgcc aacgggatcg tagatgacgt cagccccccg cccgtcggtc
     1561 agcgccttta cttcctcgcg aaacccgccc agcgtcccat cggcctgggt gtagttgatc
     1621 acatgatccg cgccacgggc cttgaccacc gccagcttct cgtcgctccc gccagttgcg
     1681 atgaccacgg cacccagacg tttgcccaga tccaccgccg ccatgcccac acctccggtg
     1741 gcaccgtgaa cgagcagcac ctcgccgggc gtcaggtcgc cacgcttggc cagcgccacg
     1801 taggcggtga ggtaggccgt ccggtacgaa gccgccttgt cgtagctcat cgagccggga
     1861 acgggctgca cactctccgc gggcacattc accgcctccg cgaaaccgcc gtagcgcagc
     1921 gccacgacca cccggtcgcc cacgtcgaac gcgctcaccc catcgccgac ggcgacgacg
     1981 tcaccggcgg cttccgtgcc gggagaaaac ggcaggggcg gcttgaactg atactttccc
     2041 tggatcatga gcagatcggg aaagttgacc gcacaggcct tcaggcgcac ctgaacctcc
     2101 ccctcacccg gcgggtcgag gctgatggtt tcgagacgga cagcgccgat gtcctcggag
     2161 atctcgtgac agcggatggc ttgaatatcg gtcacctagg cgtccttgag cagttgcttg
     2221 gcaatgttga gcagatgaat ctggctggta ccctcgtaga tccgaaacgc gcgaacgtcc
     2281 cgatagaacc gctcgctgcc gcggcccttg acgtagccct gcccaccgaa gatctgcacc
     2341 acccggtagg ctaccgagca acgggtcagc tccatgaagg cgagcgcaag ctcctcgctg
     2401 gtgagaccgc tgccgccata ggcctcaggg atgctccggc cgaaggtacc gagattacgc
     2461 atcgcctcca cgccctccgc cgggatctca tcgtccgcct ccacacgctc ctcgttggga
     2521 acggcaacct ccgcgacgaa tcggcgcacg gctgccagca gatgctcgaa ggtttctgcg
     2581 tccctgatca tcagcccgac gaccccagat tgccggcggg agccacctga aagcctgcca
     2641 tcggaaagtg gttcatgatc ggcccggtag ccgcgtcctc tcgcaagatc acgacctcct
     2701 cgcaatccca ggcaacgagg gatccggcca cgggcactcg tccgtagtcg gccggcgtca
     2761 cggtgacacg ccgacccaca tcgagctcga agggagccgc aagcgggctc tccagtaccg
     2821 gggcgacggg actgcaggac cgcgcgtgct cgagcgcagc ctcgccgctg cttggtgcaa
     2881 cggatccgtg accgaagccc gccatggtct gcatccagcc gcgaacgttc ccataggcgt
     2941 ccagcagagg agcgttcagc ggattgtttt ccagaaacca cagacaatga taaaagcaga
     3001 agtcggcgat gcagggcttc ccgccgaaca ggaacccgcc gtcgagcgcc cggtctgcct
     3061 ggtcgaggta ggtggccaga taggcctgcg ccgcttcagg ggagaacgag accatcggcg
     3121 catcacccac gagctcggcc cggtccttct gaaaggctgc aatctcttcc ggggaaagct
     3181 ggctcatgaa accgccgacc gcctccggcc ggaagttgag cgcgaccgtc acgcggaaca
     3241 gttgggtatc cgcccactcc gcgacccggt tggccgtaaa gccggccgca aacagatctt
     3301 cgtcgccgaa atgccgggcg agccccctgg caatgaccgc ggtatcgcag tagacgttgg
     3361 caccgatctg cagcacgggc gtgcgtcgat agcctcccga cagcggcatc agcaaagggc
     3421 gcggcatgat gctcggtatc tcgactgagt gccaggaacc gcccaggtac cccagaagca
     3481 gccggatctt ctccgaaaac agggaagccg gatagtggtg caggtaaatt tccaactgtt
     3541 cccccagaac ggaccttcgc ggtacgcgcg cgaccactgc gtttgccgcc cgggacacac
     3601 gcgtgctccg tacccggcct caggtcccgc cgatggtcat ctccgccacc ttgatggtcg
     3661 ggccgttgac gtagcggcga tggtccaggt cgtcgccgat catctccacc tgctgcagca
     3721 gcgtcccaag attagcaccg atggtgattc cggcaaccgg gtaagcgagt tcgccccctt
     3781 cgatccagat gccgaaggcg ccccgggaga tgtcgcccgt ggtgggcacc gccccgaacc
     3841 cgatgctgcc cgtgaggagc aggccctcgt ccactgaacc gatgatgtcc gcaggagaat
     3901 cctttcctga cgctagatag agattgttcg gccccgcccc gttccccgtg ggcttcatcc
     3961 ccagctttcg agcggagtag gagtcgagca ggaagctctt gagcacccct ttttcaatca
     4021 ccgccgtctt gcgcaggggg accccctcgc ggtcgaaggg cttcgcgccc ggcgcgcccg
     4081 gcatcagacc gtcgtcccat acgttcacgt tcgccccagc cacccgctcg ccgatccggt
     4141 cggccagaaa gctctgcttg cgatacacac cgcggccatc gacgcagctc gcgagaaact
     4201 gcaacagctc tgcgctcatc tccggctcca ggaccacggg cacgttctga gtaggaacct
     4261 tgcgggcgcc aatcaggcgg gtagatcgat cgatggcctc cgccgcgatg tcctccgggc
     4321 tcctcagccc ttcgaggtta cgggagtagt cgtactttcc ctcgctgatg aggttgtcgg
     4381 gctcccccac ctggaggtac acgccattgg aacaggccgt ctgccggaac gcgtcgctga
     4441 acccgttgga attcgcgaga tacagcgtct ctatggtggt gccgaagccg gcacccatgg
     4501 atcgcctgat tcgtttgtcg ccaaggcaaa tggcctccac gcggcgtgca gccgcgatct
     4561 tgctctccgg agacatgtcg accaccgcct cgtcgaatat gtcgagggct tcggctcgta
     4621 ccttgaccgg ctccttctcc ggcaaccctg caaacgggtc cgcgccggac aaccgcgccc
     4681 gtccgatccc gttgtcgatc agccgcttca acgtcgcctc cgacagatcg gatgacgcct
     4741 ccgtggcgac cttctggtcg acgatcagct tcaccccgag ctcccggaaa cccgcctcca
     4801 ccagggtttc cagctcgcct tccctgatgg acgcagagaa gttgctgccc gtgcggatgg
     4861 caacctcgac ctgatccgca ccacgccggg ttccgtaagc aaccaacgac tcggcgagcc
     4921 ccgtcaggct ggtgtcgcgc atcatgagcg gccacccacg gtcatgctgt tgatcttcag
     4981 agtgggcgtg cccgccgtca cgggcgcgct ctgaccgccc ttgccgcatg ttcccaggaa
     5041 cgtgcccatg tcgttgccca ccatggtcac ctcgggaagc acgtccagaa tgctgccgat
     5101 cagcgtggcg ttcttgaccg gcgcggtgag cttgccgtcc tcgatgaggt aacccatgtt
     5161 gaccgagaag gtgaacttgc ccgaaggctg caccatgccg ccctggtagg tcttggcgaa
     5221 gaacccgcgt ttgacggacc ggacgatttg ttccgggtcg gactctccgc gcgcgaggtt
     5281 cgtgttggcc attcgcggca gcgggacgtt ctcgaaacca tcacgacggc cgtggccgct
     5341 cggcgacatc ttcatgagct ttgccgagag acggtcctgc aggtagccga ccagcttgcc
     5401 cttctcgatc agcatggtct ttcggggcag cgtgccttcg tcgtccatgt tgtggctgcc
     5461 cctgaagtgc gcaatggtgg gatcgtcgta gatggtgacc aggggattgg cgacggtctg
     5521 gttcagcttg tcccagaaca ccgaccgctt tttgcggttc gagtccgctt ccagcgggtg
     5581 gccgaccgcc tcgtgaatca tgacgccgga ttgaccggaa tcgagcacca ccgtctggtc
     5641 ccccggtcgg gggctgaccg caccgagcaa cacgatggcc tcctcggccg cctcccgacc
     5701 gagctccttc ggcgtgcgcg ccgaactgaa aaaagcggat cccactcgcc caccggccgt
     5761 cgacagtccc gtgctgcgat tgcctccgtc ggccgcggtg accgaggcaa tgagtcggac
     5821 ctgcggccgc gtatccgatg ccagcaggcc ctcgctgttg gcaaccagga cgtactgaat
     5881 ctcgtctctc agagacaccc gcaccttctc gatcttcggg tggtaggcct gagcggcatc
     5941 gtacgtctgc ctaaggagga tcattcggct gctcagggaa ctctctgcaa acggtcgttc
     6001 gaggtcgtag acctgcaagg cgttggcgcg gagcctcaac ggtgcaatct ccaccctgcc
     6061 cccttcagag gcgatcgccg cggccgtgcg cgctgcctga ctcatgccct cgaaggtgag
     6121 gtcgctcgtg tacccgtagc cggtctgctc tcccttgagc acccggatgc cgacaccgag
     6181 gctgacccgc tccgaggact ccttgacgat gtcctcctcc atggtgaccc cgttggcgat
     6241 cttgtactcg aagaacagct cggcaaactc gccccccttg ccgagcgcca gcgacaggag
     6301 cctgcccatg tcgccacggc tcacgccgaa gcgcgtttcg aacagggctt ctgccgtggt
     6361 gggcacctcc tctgcgagca gcaggtcggg ccaaagggac gccgtggagg cggccgcgac
     6421 cgcagagccc gttttcttca gaaagtcgcg tctgtctgcc cgcattgtga cgagttctcc
     6481 tttttcactg atgtagcgcc ctcagtcggc agcatcgctc gaaccagccg ggttcaggag
     6541 gcgggaagat cgatccgaac gtcgccgtcg agttccccaa gcagctccac gtcgcgctga
     6601 tacacatccg ggtagaagcg gacgactccg tcgtcgtcca cctgggcggt ccagtagagg
     6661 atatagacgg gccacggatc cgcaaggcgg acccgccggg tctcaccggt ttccaccgcg
     6721 gcgcgcaccg cggcttcgtc ccagttcgcc ggatcgcgaa gaagcagggc ggcgagggaa
     6781 aacggatcct ccaccctgat gcagcccgag ctgaacgtac gctccgcccg ttcgaagagc
     6841 tcacggttcg gggtgtcgtg gatgaagacg aagtgctcgt tgggaaacat gaacttgatc
     6901 aggcccagcg cgttcgtcgg gcccggctcc tgccggaaga tgtaaggcat gccctcgaga
     6961 gcctgccagt ccacggcgta ggggtcgacg gcgcgacccg atcggtccag cagcgtcatg
     7021 ttggagcggg ccagatatcc aggatcctcg cgcaccttcg gcaggatgtc cgcccgcaga
     7081 attccgggcg gtaccgtcca ggtggggttg atttccagat aggtcataag gccgcgaaac
     7141 accggcgtct ggcgataggt ccggccgacc atggcccgac gctgccagac catctcacct
     7201 tcttccagaa cgtagacctc cgcggccgcg atgttcacga cgaccaccga cgtgtcccgc
     7261 gcggtcatga tccatcgcgc cctttcaagg gacaggcgca gctggtcgat ccgctcctcc
     7321 acggagacgt tcatggcgcg caccgtagcg ggaccaacgg ccccgtccac cgacaggctg
     7381 tggcgatgct ggaaccgcct gacaccgctt tccaggtcag cgtcgaacag ttccgggtcg
     7441 gacactgcgc ccgtctcgag atcgcccgtt acggccagcc gccggcgaac gctgacaacc
     7501 cgctcgtcgc gcatgccgat ggtcaatgtg acgccctcgg ggacgggctc ccatcccccc
     7561 gccgcggcgg cggaccggta cctcgcgagc gccatctgga tgttacggta gagggggcca
     7621 cgcgggacgc gagcagccag cccttcgcga agactccccg actcgacgat ctggttcatt
     7681 gtgtccgcag catcgacgcc cggcgcgagc tgccggccaa agttccacgt gctcacgagc
     7741 gtcttcggat tgaccttgcc gaaccgctgg tgataggcga aacgcaacag gcttgaggtg
     7801 tacagaagct cgaggtcgcg ctccgggact gccttcgggc tcgatgccgc ccgttcgcgc
     7861 aaggcgcgca aacgcgtgag gttgtagtcg tcgggatcga ggccgtcgtc cacactccgg
     7921 gccaggagat cgaacagctc ggcgacgagc gccgggtcac cccacgccgg ccgatagccc
     7981 cgacgagcgt aaaactcggc cagcaccctc tcggtcagca gggcctgccc ctggaccgcc
     8041 atggcaccgc tgctgtagta gtcgtcgaaa aattcccgca gctcgaaggc gaccacctcc
     8101 gcgccgacgc gaagcggcac ggcgaaggca agaacggcga ctgccaccgg caggctgaaa
     8161 cggagcactc gaagcatgat tccgggccgg atcaacgggc gagccaaggt agcaatctcc
     8221 acacagcagt acaaactgca ggcttgccgg gcgggcgccg ggaactttag agcgccaaac
     8281 ggatcctata ttgttggaca atccagggcg tgagtccgtc caaactcccg aaaaacagag
     8341 acgctccccg atgaaatggc tgatctgcct gcttgttgcc ggatttagcc ttccggccgc
     8401 cgcctatcgt gaagccacgg tacctttcgg cgtgaaggtt caggggatcg agaaccgatt
     8461 cgccgaattc ggcgtgtacg cgcggcctgg agagatcctg gctttagacc ttatcgacgt
     8521 cgcccccgaa gagatcggcg caaccttcga ctcggccccc gttaaaacgg gtcacggccg
     8581 gatcaacctc aaaatgccag gcgcgccggg aacccatcgg gtggagctga ccgaccagcg
     8641 cagcgggtcc aggatgcggc tcaacgtctt cgtgatgtgg caacacgacg gggcgcgtga
     8701 cgacgtgctc aacggttacc gcatcggtca gtaccccagg gatcctttcc gcggcctggc
     8761 cgtctaccgc ccgcccgagg cgttcgtcga ggtcaccccc gacaacgcgc acacgcgcgt
     8821 ctcgccgaac ttcaccctgg ggcagttcgt ctgcaagcag ggttcgggat tccccaagta
     8881 cgtggtcctg cgcccacagc tggtgcagaa gctggagctc gtgctcggtg agctgaacgc
     8941 cgtcaacccg gacgtcacgg gcctgacggt gatgagcggc taccgcaccc cggcctacaa
     9001 cagatccctg ggcaacggcc ggcacagccg gcacctttgg ggcggcgccg ccgacgtcta
     9061 cgtggacgcc aacggcgacg gcatcatgga cgatctcaac ggcgatggcc ggcgcgaccg
     9121 cggcgacgcc gagtggctgg cgaccttcat cgaccgactg gagcacgcag ggcgcttcgg
     9181 cgagcacatc ggcggcatcg gcacctacaa cagcaacgcc caccacggcc ccttcgtcca
     9241 cgtcgacgcg cgcggttacc gggctcggtg gtgaagcggc gaacgcgacg gcctgcctgg
     9301 cctgagcgcc gccgcccgaa cgtgggcccg catcaagcgt ccgaattgac ggccgcttgg
     9361 ctcacgactc agagctacag accatccagg aaacgctgca gagcctcgtt agtcgcctcc
     9421 ggtgcctcct gctgaaccca atgcccggca ccggcgatga tcgtcgatcc gcggaaatcg
     9481 gcgcacgcag cgcccggatc cgcgtacaga tccatgccgg gcacgaaacg tcgcaccgcg
     9541 tcgcgctcgc cgccaacgaa gcacgcaggc tgctccacgg gcagccccct taacggcccc
     9601 gactcggcaa cgtcgaggtt ctgcgcgcga tagcggttga tgggcccccg gaaaccgccc
     9661 gcggtaaacg cgctcacgta gatccccagg tcttcctcgc tcatccattc ggggaaggga
     9721 cggggatcga ccatcccatc cagcagtgag gcgtcggccg gcttgttttt caaccaatcg
     9781 tccagcggcg catcaccaga cagcgagaag tagatcttgc gcagcgcggt ctcgaagtcc
     9841 ccttccacct cggcctccac gacgccctcc tcctgaaagt agagctggta gaagaaccgg
     9901 ccggcgtata tcccgcggaa cagatccagg gtgccaacgt cgccggccgg caggtagggc
     9961 acgctcaagc ccgccacggc gcgcacggac tccggatgcc gcagcgccgt gttccagacg
    10021 atgggggcgc cccagtcgtg accgaacagc acgacgggct cgtcgctgag ctctcgcgcc
    10081 acggccgcca cgtccgcgca aagctcggtc atccggtagg cttcgatggc gtgaggtttg
    10141 gagctgccgc cgtaaccgcg cacgtccatg gcagctacgg tgtatccgcg ctcggcaaag
    10201 tgcctcagct gatggcgcca cgagtaccag agctcaggcc agccgtgcac gcaaagtatc
    10261 accggcccgt gcccggaaag cgcgacgcga agcacgatct cgccgttttc gatctcccgg
    10321 aattccattc cccctccccc gatcagctgg ccggttcccc gccgagcccc tgttcgcagc
    10381 accccctctt cggcgcctgt ggccgggcgc cggacgggcg gcaacctagc accagctggc
    10441 ttcctgttcc gggtggcgca gcagctcctc gatatttgca ttcagaatgc ggtagccgac
    10501 gttgccgtac aacacctggc gaccctcgtt catcacccag gacgggcttc ccctgatgcc
    10561 ccgctcctcc gccttgcgat agtccgccag aagggcggcc atggccgcgc cagaatccac
    10621 gtgtcgcttc agttccgcgg cggcgagacc aagcccctga gcaacctcca ggcagacgcc
    10681 gagatcgccg acgtctcgag catcgacgaa gaacgcctcg cgcagggccc gagacaggcg
    10741 agtcatctcc agtgggctct ccgcaatctc cgttgccttg agcaccagat gggcggtgcc
    10801 cgacgtcacc ggcctgacct tcgtccagag atactcgtgg acggcaggcg cctcgtagcg
    10861 gatggcaacc tcgcgcacgt gctgcgcaaa tccttcgtag ccgccacggg cgctccaacc
    10921 cgctgccacc ttcgcgcctg cgtcgccgaa gacgtcgacg cagtggaagc ccagccgaac
    10981 cgtctcgccc cattggtcgt tcatctcgtc gacgcgccgc tgcgcgatcc aggcccacac
    11041 gcacagcacg tcggagaagt agtcgatggt caggcggtcg ttcaggacag attctcgccg
    11101 gttccgtaca cgtcggcccc atgataacgc agcacgcaag agctccgcgc gaccgcaacc
    11161 cgggaaagga gatctgtggt cagccaagcc cagcaacctg tgtcgcacgt taccagtttg
    11221 taagtgagac tagcgatcaa ccaggccttc tcctatcttc tgaccatgca gagcggcatt
    11281 cccctttcag gcaaagcgcc aaatctattc gggctgcagc tcgctctagt gagcgccctg
    11341 ctcgtgagcg agatgttctt catcagctac cgctacgacg cctggacgct gctgcaaact
    11401 accgacagtc cctggctcac ctggctcgct tacggcggct actttcttaa gagcgcgatt
    11461 gccttcgtgg ccgcgctgct cgtcacgatt ggcacgcggc tcaaagccca ctacgacgat
    11521 ctggtgaccg tgacggcgcc ccgctcgcca tgggcgcttc tcctcgccca gcttgccgcg
    11581 tacgccgtcc tggtctggct gacgcacctg atctttgcag atccggcgga gggcgccccg
    11641 cccggcgcga tcatcgtcgg cgcatgggtt ctggccacca ctgcgacggt ttgcatctgg
    11701 ctctcactga tcgccccgtt ctcctactgg cggcgcctgg tccgccagga acgcacacca
    11761 atcgcggctt ccgtgctcgt cgcctccgtg gcgctggtca tcggtgcgtc atctcagcag
    11821 ctgtgggggc cgttgagcca gatgacgttt gcttctgtag aatgggtgct ctggctgatc
    11881 gacgatgagg tttacgtcga tcgagagcgg atgatactgg gcctgcccta ctttcagagc
    11941 cgaattgcgg agccctgctc aggatacgaa ggcatcggcc tggtcaccgt tttcaccacg
    12001 ctctatctcg ccatctaccg ggaccacttc aagtttccca acgccctgtt gctgtttccc
    12061 atcgggatca tcgccatctg gtgcgccaac gttctgcgga tcgtcgcgtt gatcgtcatc
    12121 ggcaacgact tctccccctc catcgcaatc aa
//
LOCUS       DBNHLM010000279         2315 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_340231, whole genome shotgun sequence.
ACCESSION   DBNHLM010000279 DBNHLM010000000
VERSION     DBNHLM010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_340231"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="AC16QF_12945"
     CDS             <1..1202
                     /locus_tag="AC16QF_12945"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6449515.1"
                     /translation="LPANPFLFPAPFGNPDDPQDEVNPYMQGYDNDTRKYELESWAIF
                     GELYWDITDSLSSTIGLRYSDEKKSSKQRTCYVTFLQCGPFDPEGENGYFFPKYDDNE
                     VTWKVNLTYDVNDDLMVYGTVSSSYKSGGFNPISDSDQLVIDDPSNATFEPETIDAFE
                     VGFKSTLLDRTMQLNGSFFYYDYKDLQVSKIVDVTSLNKNTDAKILGAEVEVLWAPIE
                     NLQFDLTGSWLDSEIKDFEDYDTADPKGDGSTDGLIVCLASVFLDTSPNGCGPGRIPG
                     VLQSQDGNQLAQSPEFSFNIGARYRVPMGGLGLTVATNFYWQDSFYVRNNNALHDKVE
                     SWDVWNARARFAPNDERWYAEAWIKNILDNDYVVQQYLSTQVSGLFTNQFLLDPQTFG
                     LTVGFNW"
     gene            complement(1427..>2315)
                     /locus_tag="AC16QF_12950"
     CDS             complement(1427..>2315)
                     /locus_tag="AC16QF_12950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6449516.1"
                     /translation="FTLKKGETLGLVGESGCGKTTVGKGIMRLVDITSGQVIFKRRKG
                     QSVDLVPLDRGQMRPIRSEIQMIFQDPYSSLNPRMTVGEIIGEGLRLHANLGVREVRE
                     RVADWLKHVGLEPDHMSRYPHEFSGGQRQRIGVARALILEPEFVVCDEPISALDVSVQ
                     AQVINLLDDLKRSMGLTMLFIAHDLSMVRYVSDRMAVMYLGSVVEIGPANDVFFNPKH
                     PYTEILVGSNPEPDPEAERHRASTAIVGEIPSPVNVPEGCRFANRCPKVMDVCRSVTP
                     ELIPLKNAEAVREVACHLY"
BASE COUNT          443 a          731 c          711 g          430 t
ORIGIN      
        1 tactgcccgc gaatccgttt ctgtttcccg cgcccttcgg taaccccgac gatccacagg
       61 atgaagtaaa cccctacatg cagggctacg acaacgacac ccgaaagtac gagctggaga
      121 gctgggccat cttcggtgag ctctactggg acatcaccga cagcctgagc tcgaccatcg
      181 gtctgcgtta ctccgacgag aagaagagct ccaagcagcg gacgtgctac gtcacgttcc
      241 tgcagtgcgg gcccttcgac ccggagggcg agaacggcta cttcttcccg aaatacgacg
      301 acaacgaggt cacctggaag gtcaatctca cctatgacgt caacgacgac ctgatggtgt
      361 atggaaccgt gtcgtccagc tacaagagcg gcggcttcaa ccccatcagc gacagcgacc
      421 agctggttat cgacgatccc tccaacgcca ccttcgagcc cgagaccatc gacgctttcg
      481 aggttggctt caagtcgacg ctgctggaca ggaccatgca gctgaacggc tcattctttt
      541 actatgatta caaagacctt caggtgtcca agatcgtcga cgtcacctcg ctcaacaaaa
      601 acaccgacgc aaagatcctc ggggccgagg tcgaggtgct ctgggcgcct atcgagaacc
      661 tgcagttcga tttgacgggc tcttggctcg attccgagat caaggacttc gaggattacg
      721 acacggccga cccgaaagga gatggcagca cggatggatt gatcgtctgt cttgccagcg
      781 tgttcctgga cacgagcccc aacggctgcg gtccgggccg gattccgggt gttctgcaga
      841 gccaggatgg caatcagctc gcccagtcgc ccgagttcag cttcaacatc ggcgcccgct
      901 accgcgtgcc catgggcggg ctgggcctga ccgtggccac caatttctac tggcaggact
      961 ccttctacgt gcgcaacaac aatgccctgc acgacaaggt ggaatcctgg gacgtgtgga
     1021 atgcgcgggc tcggttcgcg cccaacgacg agcgctggta cgcggaagcc tggatcaaga
     1081 acatcctcga caacgactac gtggtgcagc agtacctgtc cacgcaggtg tcagggctgt
     1141 tcaccaacca gttcctgctg gatccgcaga ccttcggtct gaccgtcggg ttcaactggt
     1201 agggacgatc ttcagggtgc ctggatgacg ggcgcccgct gatccgaaac cccgccgggc
     1261 tcaagggcct gagcggggtt tttttatgtc cgccgcttac gaaagccgat cgtgcccagc
     1321 gcccgctgca tcggcccgcg attcgagccc gcaacttgtg cgggcctttg cttgcctcgg
     1381 atgttgatgg cccttgcatc atgaagatgg cgtccatagg cggctatcag tacaggtgac
     1441 aggccacttc acggacggct tccgcgttct ttaacgggat cagctccggc gtgacgcttc
     1501 ggcacacgtc catcactttc gggcagcggt tggcgaagcg gcacccctcg gggacgttca
     1561 ccggtgaagg gatctcgccg acgatggccg ttgacgcccg atggcgttcg gcctccgggt
     1621 ccggctccgg gttggagcca acgaggatct ccgtgtacgg atgcttcggg ttgaagaaaa
     1681 cgtcgttggc ggggccgatc tccaccacgg agcccaggta catcaccgcc atgcggtcgc
     1741 tcacgtagcg caccatggac agatcgtggg cgatgaagag catggtgagc cccatggagc
     1801 gcttcaggtc gtcgagcagg ttgatgacct gcgcctgcac cgagacgtcg agcgcggaga
     1861 tgggctcgtc gcagacgacg aactcgggtt ccaggatcag tgcccgggcg acgccgatgc
     1921 gctggcgctg gccgccggag aactcgtggg ggtagcggga catgtggtcg ggctcgagcc
     1981 ccacgtgctt gagccagtcg gccacccgct cgcggacctc gcgaacgccc aggttggcgt
     2041 ggagccgcaa gccctcgccg atgatctcgc ccaccgtcat gcgcgggttc agcgacgagt
     2101 aggggtcctg gaagatcatc tggatctcgg accggatcgg ccgcatctgc ccacggtcga
     2161 gcggcaccag gtcgacgctc tggcccttcc ggcgcttgaa gatcacctga cccgacgtga
     2221 tgtcgacgag gcgcatgatg cccttgccga cggtggtctt gccgcagccc gactccccca
     2281 ccagcccgag ggtctcgccc ttcttcagcg tgaac
//
LOCUS       DBNHLM010000280         9178 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_280218, whole genome shotgun sequence.
ACCESSION   DBNHLM010000280 DBNHLM010000000
VERSION     DBNHLM010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9178
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_280218"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..876)
                     /gene="prmC"
                     /locus_tag="AC16QF_12955"
     CDS             complement(76..876)
                     /gene="prmC"
                     /locus_tag="AC16QF_12955"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR6449517.1"
                     /translation="MTTVRAWIDRERELDLLDRHLLLAELLQTNRAGVMTYPERVLND
                     AELRTLDLWSSRRRHGEPMAYILGRKDFWGLTLRVNPTVLIPRPETELLVESALEVAA
                     AGQRLLDLGTGSGAVAIALARELANAAVSASDVSQAALKLAAANAAAHGARLTFIASH
                     WFENVTPSYDVIVSNPPYVAERDAHLTSLRAEPTAALVSGADGLDAIRYIVPAARRYL
                     NEAGWLIVEHGHDQGSSVRSLMRCSGYHEVTTRRDLAGLERVTRGRRP"
     gene            complement(873..1961)
                     /gene="prfA"
                     /locus_tag="AC16QF_12960"
     CDS             complement(873..1961)
                     /gene="prfA"
                     /locus_tag="AC16QF_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR6449518.1"
                     /translation="MSASMMAKLSDLTDRFDEVSALLSDPETMADRDRFTALSKEYAE
                     LEPLILTFRRHDVLDTELREAQELAGDEDPEMRELAREDVDRLSKALDEEEQELQTLL
                     LPRDPSDASNVYLEIRAGTGGDEAALFAGDLFRMYGKFAESRGWSFEVLGARAGEHGG
                     YKEIITRVAGKDVYSQLKFESGAHRVQRVPETESQGRIHTSACTVAVMPEVDEVDSIE
                     INKSDLREDTFRASGAGGQHVNKTDSAIRLTHLPSGIVVECQDERSQHKNRARALALL
                     QAKLLDAAQSQQRSEQAETRRNLVGSGDRSERIRTYNFPQGRVTDHRINLTLYKLTEV
                     MEGALENVIDPLVREHHADQLRSLGDEQ"
     gene            complement(1971..3224)
                     /gene="hemA"
                     /locus_tag="AC16QF_12965"
     CDS             complement(1971..3224)
                     /gene="hemA"
                     /locus_tag="AC16QF_12965"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR6449519.1"
                     /translation="MAILAYGINYRTAPIDVRERVAFPEDALPAALEDAMSSIPDVSE
                     IAIISTCNRTELIVSAAPEAEESVARWLASYRPVSMGELNSMAYSHWDQDAARHLIRV
                     AAGLDSQVLGEPQIMGQVKQAYELARAAGALGSELSLLSRLTLNAAKRIRTDTDIGRN
                     PVSVAYAAVTMAQQIFADLHHNKALLIGAGDTIQRVAEHLSALGTAGLAIANRTLANA
                     EALAAGFDAVPMQLTDIAAHLADYDIVITSTGSSLPVLGKGTVEAAIKRRRHKPMFIV
                     DIAVPRDVEPEVAELPDVYLYTIDDLTEIIEENIKQRRSAAESAEHLVMEGAEAYTRE
                     RRAHEGQALLRSFRENAEAIQRAELEKALTALGRGGDPNEVLKSLSRSLTNKLIHQPT
                     VAIRDASASDRSDLLEYLKSLYQLD"
     gene            3333..3863
                     /gene="lolB"
                     /locus_tag="AC16QF_12970"
     CDS             3333..3863
                     /gene="lolB"
                     /locus_tag="AC16QF_12970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HIR6449520.1"
                     /translation="MALCAGCASFRPPVETPADFEVRGKAGVVEGAESFSARFVWRQY
                     GSAFTIDLWGPLGQGRVRLDGDERRLAIVDGKGAVISEGPHAEVMRTHLGWSLPLDVL
                     PSWVLGKPGEGTGAGGRQYDDDGRLVAFEQLDWSVAYARHNQVQTARGSRWLPGRITA
                     VRGDYRVRLSISDWVL"
     gene            4013..4087
                     /locus_tag="AC16QF_12975"
     tRNA            4013..4087
                     /locus_tag="AC16QF_12975"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4045..4047,aa:Gln,seq:ttg)
     gene            4154..5098
                     /locus_tag="AC16QF_12980"
     CDS             4154..5098
                     /locus_tag="AC16QF_12980"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR6449521.1"
                     /translation="MSNLMIFTGSSNPVLSARIAEELRMDLGHADLGRFSDGEVNVEI
                     HENVRGQDVFVIQSTCAPTNDNLMELMIMSDAIRRASAQRVTAVIPYFGYARQDRRAR
                     SARVPISAKVVADMLGSVGIDRMLTVDLHADQIQGFFNMPVDNVYGSPVLIDHMLGEH
                     YENPIIVSPDVGGVVRARAVAKQCNDLDLAIIDKRRPQANDSRVMNVIGDVRGKTCIL
                     VDDIVDTAGTLCTAAEALKEEGAIGVVSYITHAVLSGPAIERIAGSELDRMVVTDTIP
                     LSGSAESCSKIEQLSLARLLAEAIRRVSNEESISAMFR"
     gene            5168..5857
                     /locus_tag="AC16QF_12985"
     CDS             5168..5857
                     /locus_tag="AC16QF_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HIR6449522.1"
                     /translation="MTQRIEIHAELREDVGKGASRRLRRLADKVPGVIYGGDADPVPL
                     MLSSNELSKAMQLEAFYSQILNVVLDGKGEQAVVRDLQRHPASEKVQHIDFLRIRADR
                     LIQVSVPLHFIDEDKCIGVKQGGGQIVHNMNEVEISCLPGDLPEFIEVYMAQVDVNQS
                     VHLTDLALPEGVSIVALMHGSDRDANVVSVQMPRGGLEEEEAAEAAEAAAEEGGEASD
                     EGAGGEEASED"
     gene            5860..6468
                     /gene="pth"
                     /locus_tag="AC16QF_12990"
     CDS             5860..6468
                     /gene="pth"
                     /locus_tag="AC16QF_12990"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR6449523.1"
                     /translation="MADVSLIVGLGNPGPRYELTRHNAGAGFVEYLANRFGVVLRDEA
                     RFRGRMGRGEVLGHDVRLLIPTTYMNLSGSSVAAVARFYKLAVDEILVAHDEMAFEPG
                     MLRLRTGGGDNGHNGLRSIISQLGNAAGFPRLRIGVGHPGDRGRVEAYLTTERMPESE
                     RGQMEAAWRMEDAVLAAILDGDLQAAMNALHAPAGAGDEGED"
     gene            6469..7560
                     /gene="ychF"
                     /locus_tag="AC16QF_12995"
     CDS             6469..7560
                     /gene="ychF"
                     /locus_tag="AC16QF_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR6449524.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIDAENFPFCTIDPNTGVV
                     PVPDPRLERVAQLARSAKIVPTSMEFVDIAGLVAGASRGEGLGNQFLAHIRETDAIAH
                     VVRCFEDDNVTHVSGAVSPADDIEIINTELALADLETAERVFDRTRRIANAGDKDAKL
                     LVAVLDKVIGGLNTGRPVRALALGPEELKLLHECHFITAKPVLYVANVAEDGFDNNPL
                     LDGVRAIAAAESSEVVVICNKIEAEIVELEEDERAEFLEDLGIEEPGLNRVIRAGYRL
                     LGLHHYFTAGPKEARAWTVRIGTRAPQAAGVIHTDFEKGFIRAEVIAYDDYVGCGGEQ
                     GAKDTGKWRLEGKDYVVQDGDVILFRFNV"
     gene            complement(7608..8381)
                     /locus_tag="AC16QF_13000"
     CDS             complement(7608..8381)
                     /locus_tag="AC16QF_13000"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6449525.1"
                     /translation="MDNPRTALITGASAGIGKAFADALAAGGVNLVLVARRADRLQQA
                     AQALREAHGIDVATISADLSDPTAPVRIHEETESRGLVIDYLINNAGVAGPDLLEPDT
                     WQANAGFHQLMMIAPAHLCHLYVPAMRARRWGRIVNVASVAARIPRAGGCNYGPAKAF
                     LVALSEELSLTLKAEGIKVCALCPGFTHTEFHNEGSAAELKANTPAFVWYEAETVVKE
                     GLRALERGRSVYVSGRMYRWMDPFFQSVWTRRLFKIAAS"
     gene            8466..8542
                     /locus_tag="AC16QF_13005"
     tRNA            8466..8542
                     /locus_tag="AC16QF_13005"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8500..8502,aa:Met,seq:cat)
     gene            complement(8585..9136)
                     /locus_tag="AC16QF_13010"
     CDS             complement(8585..9136)
                     /locus_tag="AC16QF_13010"
                     /inference="COORDINATES: protein motif:HMM:NF016763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR6449526.1"
                     /translation="MGEVLGLALYDRALTATEVEQHANAGPGSGFAELARNSGAVGAY
                     QFDAGGGTSVQDQAGGGHDLKIPEYFRPPGRGLLRSVDPAERTALWNVGDAILNVLGF
                     APLGFLISIVLRKHVKPTSLILTATMVGFVVSLGIEWTQSLMITRSSSLQDLVLNTVG
                     SLVGAVAALSSSRFRGRENNTKA"
BASE COUNT         1593 a         2878 c         3025 g         1682 t
ORIGIN      
        1 cctggccggc gacgtcgacc tccggcagca tgatctggcg gctgtatcga agcagctgat
       61 cgtcgctcag cccattcaag gtcttcggcc tcgcgtgacg cgctccagcc cggcgaggtc
      121 ccgtcgcgtc gtcacctcgt ggtagccgct gcaacgcatg agcgaacgga ccgagctgcc
      181 ctgatcgtgg ccgtgctcga cgatgagcca accggcttcg ttgaggtagc gcctggcggc
      241 gggaacgatg tagcgaatgg cgtccagccc gtctgcgccg ctcaccagcg cggcggtggg
      301 ctcggcccga agcgaggtga gatgggcatc gcgctcggca acgtaaggcg ggttgctgac
      361 gatgacatcg taggaagggg tgacgttctc gaaccagtgg ctcgcaatga acgtcagcct
      421 ggcgccgtgg gctgcggcat ttgcagccgc gagcttgagc gctgcctggg agacgtcgct
      481 tgcgctcacc gccgcgttcg cgagctcccg ggccagcgcg atggcgacgg cgccgctgcc
      541 cgtgccgaga tccagtaggc gctgaccggc cgcggctacc tccagcgcgc tctccaccag
      601 cagctccgtc tcgggtcggg ggatcaatac cgtcgggttc acccgaagcg tcagccccca
      661 gaaatccttg cggccgagga tgtaggccat gggctcgccg tggcgccgac ggctcgacca
      721 gaggtccagg gtgcgaagtt ccgcgtcgtt cagaacgcgt tccggatagg tcatgacgcc
      781 ggcgcggttg gtttgcagca gctcggcgag cagcaggtgg cgatccagca gatccagctc
      841 ccgctccctg tcgatccagg cacggaccgt ggtcactgct cgtcgcccag gctgcgcagc
      901 tgatccgcgt gatgctcacg caccaggggg tcgatcacgt tttccagtgc cccctccatg
      961 acctcggtca gtttgtagag ggtcaggttg atccgatgat cggtgacgcg accctgggga
     1021 aagttgtagg tgcggatgcg ctccgagcgg tcgcccgagc ccaccagatt acggcgcgtc
     1081 tccgcctgct cggatcgttg ctgcgactgg gcggcgtcga gcagcttcgc ctggagcagc
     1141 gccagcgccc gcgcgcggtt cttgtgctgc gagcgctcgt cctgacactc cacgacgatg
     1201 ccgctcggca ggtgagtcag ccggatggcc gagtccgtct tgttcacgtg ctgtccgccg
     1261 gccccggagg cgcggaaggt gtcctcgcgc agatcggact tgttgatctc gatggagtcc
     1321 acctcgtcca cctccggcat cacggccacg gtgcacgcgg acgtgtgtat gcgtccctgg
     1381 gactccgttt cgggaacgcg ctgcacccgg tgcgcgcctg actcgaattt cagctggctg
     1441 tagacgtcct tgccggcgac acgggtgatg atctccttgt atccgccatg ctcgcctgcc
     1501 cgggcgccca gcacttcgaa gctccagccg cgggactccg cgaacttgcc gtacatgcgg
     1561 aagaggtccc cggcaaacag cgccgcctca tcaccacccg tacccgcgcg aatctccagg
     1621 tagacgttcg acgcgtcgct ggggtcccgt ggcagcagca gggtctgcag ttcctgctcc
     1681 tcctcgtcga gcgcctttga caggcgatcc acgtcctccc gcgcgagctc gcgcatctcg
     1741 gggtcctcat ccccggcgag ctcctgggct tcgcgcagct ctgtgtccag cacgtcgtgg
     1801 cggcggaacg tcaggatgag gggctccagc tcggcgtact ccttggacaa cgcggtaaac
     1861 cggtcccggt cggccatcgt ctcgggatcc gacaggaggg cgctgacctc gtcgaagcgg
     1921 tcggtcagat ctgacagctt ggccatcatc gaggcggaca ggctcatgat tcagtctagc
     1981 tggtagagag acttgagata ttctaagaga tcgctgcggt cactcgcgct ggcatcgcga
     2041 atcgcgacgg tcggctggtg aatcagcttg ttggtcagag accggctcaa tgacttcagc
     2101 acctcattcg ggtcgccccc ccggcccagt gccgtcagcg ctttttcgag ctccgcccgt
     2161 tggatggctt cggcgttctc gcggaaactg cgcagcagcg cttggccctc gtgggcgcga
     2221 cgctcgcggg tgtaggcttc ggcgccttcc atgaccagat gctcggcgct ctcggcagcc
     2281 gatcggcgct gtttgatgtt ctcctcgatg atctcggtga gatcgtcgat ggtgtagagg
     2341 tacacgtcgg gcagctcggc cacctcaggc tccacgtctc gcggcacggc gatgtcgacg
     2401 atgaacatcg gcttgtggcg ccgacgcttg atggcggcct ccacggtccc tttgccgaga
     2461 acgggcagtg agctgccggt ggacgtgatg acgatgtcgt agtcggcaag gtgagcggcg
     2521 atgtcggtca gctgcatggg gacggcatca aagcctgccg caagagcctc cgcgttcgcc
     2581 agggtccggt tcgcgatggc caggcccgcg gtgcccagcg cggaaaggtg ctcggccacc
     2641 cgctgaatcg tgtcgccggc gccgatgagc agtgctttgt tgtggtgcag gtcggcaaag
     2701 atctgctgcg ccatggtgac cgcagcgtag gccaccgaga cggggttgcg gccgatatcg
     2761 gtgtcggtgc ggatccgctt ggcggcattg agcgtaagcc gggacagcag gctcagctcg
     2821 gaaccaagag cgccggccgc cctagccagc tcgtaagcct gtttcacctg gcccatgatc
     2881 tgcggctcgc cgagcacctg agaatccagg cccgccgcga cacgaatcag gtgtcgcgct
     2941 gcgtcctggt cccagtggct gtaggccatg ctgttcagct cgcccatgct cactggccga
     3001 tagctcgcca gccaacgggc cacggattcc tcggcctcgg gcgctgcgga gacgatgagc
     3061 tccgtgcggt tgcacgttga gatgatggcg atctcgctga catcgggaat agacgacatg
     3121 gcatcctcta gggccgccgg cagggcgtcc tccggaaatg cgacgcgctc ccggacgtcg
     3181 atgggagccg tgcgatagtt aatgccgtat gccaatatcg ccatgttgtg cagggtatga
     3241 tctcagctga gagccttctt gccgacccag cattctagca gcttcggaga tttgtgaacg
     3301 tacttaagcc attcatttta aagttgtttt gtttggccct ctgcgccggc tgcgcgtcgt
     3361 tccgccctcc cgtcgagacc ccggccgact tcgaggtgcg cggcaaggcc ggcgtcgtcg
     3421 aaggcgcgga gagtttttcg gcgcgcttcg tctggaggca gtacggctcg gcattcacca
     3481 tcgacctctg ggggcccctc gggcaggggc gcgtcaggct cgatggggac gagcgccgcc
     3541 tggccattgt agacggcaag ggagccgtga tctccgaggg gcctcacgcc gaggtcatgc
     3601 gtacgcatct tggctggagc cttccactgg acgtgctgcc cagctgggtg ctgggcaagc
     3661 cgggagaggg aaccggcgcc ggcggccggc agtatgacga tgatgggcgc ctggtcgctt
     3721 tcgagcagct cgactggtca gtcgcctacg cccggcacaa ccaggtgcag acggctcgtg
     3781 gttcccgctg gctgccgggc cggatcacgg ctgtgcgggg cgactatcgg gtgcggcttt
     3841 ccatatccga ctgggtgctc tgacgggccc cgatacgtgg acgtctgccc gtcaggtctg
     3901 gcggtttgac agccaacaga cccaacatca caatagcgcc ccgctgccgg ggcgagaacc
     3961 gcgaaacgtc aggaacagcg ccggcgggcg cacatggcaa cgagctgtct gttggggtgt
     4021 cgccaagtgg taaggcaccg ggttttgatc ccggcattcg caggttcgaa tcctgccacc
     4081 ccagccaaac tgttgatctc ttgaagccgc aaccgcatag cacgcgcaaa gagcgcggac
     4141 gaggggaagc caattgtcaa acctgatgat cttcacgggg agctcgaacc ccgtgctgag
     4201 cgcccgcatc gccgaggagc tgcggatgga tctcgggcac gccgacctgg ggaggttcag
     4261 cgacggtgag gtgaatgtgg agatccacga gaacgtgcgc ggtcaggacg tcttcgtcat
     4321 ccaatccacc tgcgctccga ccaatgacaa cctcatggag ctcatgatca tgtccgacgc
     4381 catccgccgc gcatcggcgc agcgcgtgac ggcggtgata ccgtattttg gctatgcgcg
     4441 tcaggacagg cgagcccgct cggcacgagt gcccatcagc gccaaggtgg tcgccgacat
     4501 gctcggcagc gtcggcatcg accgtatgct caccgtcgat ctccacgcgg accagatcca
     4561 gggctttttc aacatgccgg tggacaacgt ttacgggtcc ccggtcctga tcgaccatat
     4621 gctcggtgag cactacgaga atcccatcat cgtctcaccc gacgtgggcg gggtggtgcg
     4681 cgctcgcgcg gtggccaagc agtgcaacga tctggacctc gccatcatcg acaaacgtcg
     4741 cccgcaggcg aacgattccc gcgtgatgaa cgtcatcggc gacgtccgcg gcaagacgtg
     4801 catcctggtt gacgacatcg tcgatacggc gggcacgctg tgcacggcgg ccgaagcgct
     4861 caaggaagag ggcgccatcg gcgtggtttc ctacattacg catgcggttc tttcgggacc
     4921 cgccatcgaa agaatcgccg gctccgagct cgaccggatg gtcgtgactg acacgatacc
     4981 gttgagcgga tcggccgaaa gctgctcgaa gatcgagcag ctcagcctcg cccgtcttct
     5041 ggcggaagcc atccgacggg tgagtaacga ggaatccatc agcgcgatgt ttcgctagct
     5101 gatggttgca attggacgcc aggacttggt cgcgatccgg gacgtccgta tttgatggag
     5161 aaatctgatg acgcagcgaa ttgaaattca tgccgagctg cgcgaggacg tggggaaagg
     5221 tgcgagccgc cgcctgcgtc gccttgcaga caaagtgccc ggcgtaatct atgggggtga
     5281 tgctgatccg gtcccgctga tgctgagcag caacgagctt tccaaggcca tgcagctcga
     5341 ggccttttac tcgcagatcc tgaacgtcgt tctggacgga aagggtgagc aggcggtggt
     5401 acgggacctg caacgccatc cggcctcgga aaaggtgcag cacatcgact ttctgcgaat
     5461 ccgcgccgac cgtctcattc aggtgtccgt gccgctccac ttcatcgacg aggacaagtg
     5521 catcggcgtg aagcagggcg gcggtcagat cgtgcataac atgaacgagg tcgagatcag
     5581 ctgtctgcct ggagatctgc ccgagttcat cgaggtctac atggctcagg tggacgtcaa
     5641 tcagtcggta cacctcaccg atctggcgct tccggaaggg gtcagcatcg tcgccttgat
     5701 gcacggcagc gaccgtgatg ccaacgtggt cagcgtccag atgccgcgcg gcgggctgga
     5761 agaggaagag gccgccgagg ccgcggaagc ggccgctgaa gagggcggcg aagcctccga
     5821 cgaaggcgct ggcggcgagg aagccagcga agactgacca tggcggacgt cagcctgatc
     5881 gtgggcctgg gaaacccggg cccgcggtac gagctcacgc gccacaatgc gggtgccgga
     5941 ttcgtcgagt acctggctaa ccggttcggc gtcgtgcttc gggacgaggc gcgtttcagg
     6001 ggccgaatgg gccgcggtga ggtcctcggc catgatgtgc gcctgctcat tcccaccacc
     6061 tacatgaatc tgagtggcag ctcggtggct gcggtggcgc gcttctacaa gctggccgtg
     6121 gacgaaattc tggttgccca cgacgagatg gcgttcgagc cgggcatgct gcgtctgagg
     6181 acgggcggtg gtgacaatgg gcacaacggt ttgcgcagca tcatctctca gctcggcaac
     6241 gctgcgggtt ttccccgtct acgcattggg gtaggccatc ccggagacag gggccgtgtc
     6301 gaggcctacc tcactaccga gcgcatgccc gagtcggaac ggggtcagat ggaagccgcc
     6361 tggcgtatgg aagacgcggt gctggcggcg attctcgacg gggatctgca ggctgccatg
     6421 aacgccctgc atgcgccggc gggggcgggc gacgaagggg aggactgaat gggattcaag
     6481 tgcggaatcg tcgggttgcc gaacgtcggc aaatccacgc tgttcaacgc cctgaccaag
     6541 gcgggcatcg atgcggagaa ctttccgttc tgcaccatcg atcccaatac tggtgtggtc
     6601 cccgtccctg atccgcggct cgagcgggtt gcccagctgg cccgttcggc caagatcgtt
     6661 ccaaccagca tggagttcgt cgatatcgcc ggtcttgtcg ccggtgcgtc ccgtggtgaa
     6721 gggctcggaa atcagttcct cgcgcacatt cgagaaaccg atgccattgc tcacgtcgtg
     6781 cgttgcttcg aggacgacaa cgtcacccac gtctccggcg cggtatcgcc tgctgacgac
     6841 atcgagatca tcaatacgga gctcgccctt gccgatctgg agacggcgga gcgcgtcttc
     6901 gaccggaccc ggcgcatcgc caacgcgggc gacaaggacg ccaagttgct ggtcgccgtg
     6961 ctggacaaag tcatcggggg cctgaatacg ggacgcccgg tgcgggcgct ggcgctcggt
     7021 cctgaggagc tgaagttact gcacgagtgc cacttcataa cggccaagcc cgttctctac
     7081 gtggcaaacg tggcggagga cggcttcgac aacaacccgt tgctggacgg cgtgcgggcc
     7141 atcgccgctg ccgaatcctc ggaggtggtg gtcatctgca acaagatcga ggccgagatt
     7201 gtcgagctcg aagaagacga gcgagcggag tttctggagg atctcggtat cgaagagccg
     7261 ggactcaatc gtgtcatccg ggcaggctat cgcctgctcg ggctgcacca ttacttcacc
     7321 gccggaccca aggaggctcg cgcctggacc gtccgcatcg gcacgcgggc gccccaggcg
     7381 gcaggtgtga ttcataccga cttcgagaag ggattcatcc gcgcggaggt catcgcctac
     7441 gacgactacg tcggctgcgg cggcgagcag ggtgccaagg acaccggaaa gtggcggcta
     7501 gagggcaagg actacgtcgt tcaggacggc gacgtgatcc tcttccgctt caatgtctga
     7561 tctcggggtc ggctcgcagg attcgaggca ccggccgggt cgggcgttca gctcgccgcg
     7621 atcttgaaga gtcggcgggt ccacaccgac tgaaagaacg ggtccatcca gcgatacatg
     7681 cgaccggaca cgtagacgct gcggccccgc tcgagggccc gtaatccttc cttcaccacc
     7741 gtctccgcct cgtaccagac gaaggcgggc gtgttcgcct tgagctctgc tgcggatcct
     7801 tcgttgtgaa actcggtgtg cgtgaagccc gggcacagcg cgcacacctt gatgccctca
     7861 gccttcaggg tgagggagag ctcctctgag agcgccacca gaaaggcctt tgccggtccg
     7921 tagttgcacc cgccggcgcg aggtatgcgc gccgccaccg atgcaacgtt gacgatcctc
     7981 ccccaccgcc gggcacgcat ggccggcacg tacagatggc acaggtgcgc gggcgcgatc
     8041 atcatcagct gatggaagcc ggcgttggcc tgccaggtat ccggctcgag gaggtcgggc
     8101 ccggctaccc ccgcgttgtt gatgaggtag tcgatgacca ggccacggct ctccgtctcc
     8161 tcgtgtatgc gcacgggggc cgtcggatcg gataggtctg cgctgatcgt ggcgacgtcg
     8221 atcccgtgag cttcgcgcag cgcctgggct gcctgctgca gccgatcggc tcgtcgcgcc
     8281 accaacacca gattgacacc ccccgctgcc agggcatcgg cgaaggcctt gccgatccct
     8341 gcggatgctc cggtaatcag cgccgttctg ggattgtcca cgccgtcctc ctgcgtccgc
     8401 cgcacgttga cagctcatcg gcttgtcagg acaatcgcga tccttcgcca gcacaggcaa
     8461 gccatggcta cgtagctcag ctggttagag cacagcattc ataatgctgg ggtcgttggt
     8521 tcaagtccaa ccgtagctac caatcccttt ctcgacgaac aaccggccgt gtcatctggc
     8581 tctgttacgc tttggtattg ttttcgcggc ctcgaaaacg ggaactggaa agggccgcca
     8641 cggctccgac caggctgcca acggtgttca gcacgagatc ctgtaaagat gaagacctcg
     8701 taatcatcag cgattgtgtc cactcgatgc ctaggctgac gacgaaacca accatcgttg
     8761 cggtcagaat gagggaagtc ggtttcacgt gcttgcgtag cacaatcgat atcaagaagc
     8821 ccaatggggc aaagcccaga acgttcagaa tcgcgtcgcc gacgttccag agtgctgtgc
     8881 gctcagccgg atcgacgctc cgaagcagcc cgcggcccgg tgggcgaaag tactcgggga
     8941 tcttgaggtc gtgcccgccg cccgcctgat cctgaaccga ggtgccgccg cccgcgtcga
     9001 actgataggc gccaacggca cccgagttcc ttgccagttc ggcgaatccg gatcccgggc
     9061 ccgcgttcgc atgctgctct acctccgtcg cggtcagcgc gcgatcgtat agggcgagcc
     9121 cgagcacttc tcccatccac ggcgtgaccc agggtggccc ggcgccaacc acgagccg
//
LOCUS       DBNHLM010000281         9444 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_879836, whole genome shotgun sequence.
ACCESSION   DBNHLM010000281 DBNHLM010000000
VERSION     DBNHLM010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9444)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9444
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_879836"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /locus_tag="AC16QF_13015"
     CDS             <1..1536
                     /locus_tag="AC16QF_13015"
                     /inference="COORDINATES: protein motif:HMM:NF044325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E domain-containing protein"
                     /protein_id="HIR6449527.1"
                     /translation="DTKSLALGVLRLVEEEALKERSSIVRALVPLSIASYLLNEKRAD
                     VAQIEQRTGTHLVIVPSVNMDTPHFEIHRVRDDHAREVGIVPSYELAESAAAEEAMPL
                     QEAPRPPAPQAAVQAIQPSAPKPAPAPAQAPQPAARKPQSPSQSNRPGLFSRLRDALF
                     TSGESQAEGSTSTERQAQGERAEDRGKKDDGRRNRQRGNGGQRKDTARHNDGAESRRG
                     QPEEAKGRKRGPKKDSEEGSNARKAKGGERGGERGGERGKGSEKAQDKAKEKGKDKGK
                     DKDKGKDKAAGAGTPPTEEEITRSKRRPRRDRSQVEKSEPANGRPTAAAGGEAAPVAT
                     TTAQPAARAGAATAAPVTETPEAVSSETSAAAVEPAAEQQPQESAKVASGSSTPQGRA
                     ANDPRERNIADQPTPAAREKAPASDAAPPTPPAEPVAQAPDVPAASPDKPAAEEPAVA
                     KRPEAKGQPKDASVEPAPEAPTPEPRPSRAFNDPREVRRREREAELKAEGVIPKTGDQ
                     STP"
     gene            complement(1521..2540)
                     /gene="murB"
                     /locus_tag="AC16QF_13020"
     CDS             complement(1521..2540)
                     /gene="murB"
                     /locus_tag="AC16QF_13020"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR6449528.1"
                     /translation="MIAAPRLDALNTLGVAATADRLVVVESERALQGLLREAADQRGG
                     LVVLGGGSNVVLKRHVPGTVAVIRTRGTHIVPGRGGRVNVSVAAGESWHGLVRFTIGQ
                     GLYGLENLALIPGSVGAAPLQNIGAYGVELADRLISLRAMEVASGAVREFHREECGFA
                     YRDSRFKSADAGRFIILDVTLALSREAQPVIDYPELRLELQRLGGSRISPVLVAEAVV
                     RVRRRKLPDPRRVGNAGSFFKNPVVSAPKAEGLRAAVPGLVEHFQSDGRVKLSAAQLI
                     DRAGFKARAAGKVGVWPRQPLVLINRDGASGADVLAFGQSIRAEVQRLYGVSLELEPQ
                     VYGVD"
     gene            complement(2537..3145)
                     /locus_tag="AC16QF_13025"
     CDS             complement(2537..3145)
                     /locus_tag="AC16QF_13025"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF024922.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB
                     family protein"
                     /protein_id="HIR6449529.1"
                     /translation="MTATLPFRSTVLPSTNERASIPQAGYRHFPSYLADTPADVLLGW
                     LRESVDWRSERIRILGKSVAVPRLVAWYGDPGVCYRYSGVEHVAGGWPSQLCSVRDLL
                     RREWSAGFNFVLINRYRDGSDSMGWHRDDEPELAGPIASLSLGATRRLRVRVDGGTGS
                     IALDLSHGALLCHERHLPHALMKTRRPVGERINLSFRTVVSR"
     gene            3117..3887
                     /locus_tag="AC16QF_13030"
     CDS             3117..3887
                     /locus_tag="AC16QF_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6449530.1"
                     /translation="MDLNGKVAVITGGASGLGRATAEMVINNGGKVALFDLNEDLARQ
                     TAAALGDDAAPFAVDVTNDESAAAAVDQVLERFGAIHVNVNCAGIGAAGRTISKEGAM
                     PLKHFNFIIQVNLVGTFNTLRLCAAAMNENEPTTEDGEKGVIINTASVAAFDGQIGQA
                     AYSASKAGVAGMTLPIARDLARSGIRVCTIAPGIFETPMMAGAPDRVRDPLIEMTQFP
                     KRLGNAAEYALLARQIIENTYLNGETIRLDGGIRMSPR"
     gene            complement(3895..4659)
                     /gene="kdsB"
                     /locus_tag="AC16QF_13035"
     CDS             complement(3895..4659)
                     /gene="kdsB"
                     /locus_tag="AC16QF_13035"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003317470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HIR6449531.1"
                     /translation="MAEPFKVVVPARYGSSRLPGKPLADLHGKPMIVRVAERCAASGA
                     EEVVIATDDVRIVEAVTSAGFEATMTRADHASGSDRVMEVARLRAWDDDAIVINVQGD
                     EPQMPPAVVMQVAQILQADPARPVATLCEPIREVAVLTDPNVVKVVRDRWGRALYFSR
                     APIPYERDEAPGKSGQDRGRPIGLRHIGVYGYRLSALQQFVELPVSDLERLEKLEQLR
                     LLENGIPIFVTEACETVPAGVDTPADLERVRTRSPS"
     gene            complement(4652..5737)
                     /gene="lpxK"
                     /locus_tag="AC16QF_13040"
     CDS             complement(4652..5737)
                     /gene="lpxK"
                     /locus_tag="AC16QF_13040"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - lipid-A 4'-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HIR6449532.1"
                     /translation="MAEAPTGSTAPGLTGKLTVGQTLRPLAQAWYTGSRWTVLLAPFA
                     WAFERYAVRRRLRFLTGKRRSWRSPVPVIVVGNITAGGTGKTPMVIWLVEQLRALGYS
                     PGVVSRGYGGKRGRKPLLVTPDSDPAVVGDEAPMIASRARCPVVVAADRARAVESLLQ
                     AANSDVVVSDDGLQHYALARDLEIALIDGHRGVGNGRCLPAGPLREPVSRLESVDWVI
                     SNGLGSGIVARETVMTLIPTAFVRVTPGPGEARQRPEDFVAAHPNVNAVAGVGNPARF
                     ALALRELGLAPVLHAQPDHHPYDGSELVFDNDWPVVCTEKDAVKIRRLTDLPADVWYL
                     EVEAHLPDGAAAFLEELLRAHGISRHG"
     gene            complement(5717..7522)
                     /gene="msbA"
                     /locus_tag="AC16QF_13045"
                     /pseudo
     CDS             complement(5717..7522)
                     /gene="msbA"
                     /locus_tag="AC16QF_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285214.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid carrier activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
     gene            complement(7551..7976)
                     /locus_tag="AC16QF_13050"
     CDS             complement(7551..7976)
                     /locus_tag="AC16QF_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR6449533.1"
                     /translation="MKFTRRRQPEATVELTPLIDVVFLLLIFFMVSTTFIRETQLKIN
                     LPEASGEIQEVERDFIEITIDRRGEYAVNDRLLVNAELRTLIRALEEMVAERGESRRV
                     IVTADAQASHQAVVRAMDAAGRVGLTQISLTTKQPEEEQ"
     gene            complement(7992..8615)
                     /locus_tag="AC16QF_13055"
     CDS             complement(7992..8615)
                     /locus_tag="AC16QF_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017519262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6449534.1"
                     /translation="MLEIVQSGGWLMVPILLCSIIAAAISVERLWTLQRGRIAPTDLL
                     AEVWDAIKGDRLDAQRLKDIRASSPLGQVLAAGIGNAKRGREIMKEAMEESAAQVSHD
                     MERYLTSLGVIASISPLLGLLGTVVGMIKVFTALMLEGAGNANVLAGGISQALITTAA
                     GLSVAIPALMFHRFFLRRVDELMVTMEQEAGKLVEIMQGGPDDDPRL"
     gene            complement(8647..>9444)
                     /locus_tag="AC16QF_13060"
     CDS             complement(8647..>9444)
                     /locus_tag="AC16QF_13060"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR6449535.1"
                     /translation="RKGRVSVVVDGPLALQVLREAACPDAEVGVLVDIDVDMGRTGCR
                     DPDQVLALAESIETTPGLRYEGVQHYAGHLMHVEGHQNRRRRSTALWEQVAGVVDRLR
                     QNGLAPAVVTGGGTGTYDIDTDIEAVTDVQVGSYVFMDHEYRVIGGARGEVFDDFEVA
                     LTVAATAISKPLDSAVTVDAGYKAFASDTVAPEPMHIAGARYRFAGDEHGVVIPAAGS
                     QEPLLGEVHQFITSHCDPTVNLYDHYWVHRDGLAHSIWPVAARGCSW"
BASE COUNT         1814 a         3203 c         2979 g         1448 t
ORIGIN      
        1 gacaccaagt cgctggctct cggtgtgctg aggctcgttg aagaggaagc actcaaggaa
       61 cgcagctcta tcgttcgcgc gctggtgccg ctgagcatcg cctcgtacct gctcaacgaa
      121 aagcgggcgg acgtcgcgca gatcgagcag cgcaccggca cccatctggt catcgtgccg
      181 agcgtgaaca tggacacccc gcacttcgag attcataggg tgcgggacga ccatgcccgg
      241 gaggtgggca tcgtgccaag ctacgagctg gccgagtcag cggcagctga ggaagcgatg
      301 cctttacagg aagcaccccg ccccccggcc cctcaggctg cggttcaggc cattcagcct
      361 tccgccccca aaccggctcc cgcccccgcg caggcgccgc aacccgccgc ccgcaaaccg
      421 cagtcgccga gtcagagcaa cagacccggc ttgttctccc ggctgaggga tgcgctgttc
      481 accagtggag agtcccaggc agaaggctcg accagcacgg agcgccaggc ccaaggcgaa
      541 cgcgccgagg atcgtggcaa aaaggacgac ggcagacgca ataggcaaag gggcaacggc
      601 gggcagcgca aggacaccgc ccggcacaat gacggcgctg agagccgacg cgggcagccc
      661 gaggaggcca agggcaggaa gcgagggcca aagaaggatt ctgaagaggg ctccaacgcc
      721 aggaaggcga agggcggcga gcgcggcggc gagcgcggcg gcgagcgcgg caagggctcc
      781 gagaaggcac aggacaaggc gaaggagaaa ggcaaggaca agggcaaaga caaggacaag
      841 ggcaaggaca aagcggccgg agcaggaact ccgcccaccg aggaggagat cacaaggagc
      901 aaacgtcgcc ccaggcgcga ccgcagtcag gtcgagaagt ccgaacctgc caacggcagg
      961 cccaccgcag ccgccggtgg cgaggcagcg cccgtggcga ccaccactgc gcagcccgca
     1021 gcgagggcag gcgccgcgac cgcggcaccg gtcaccgaaa cgcctgaggc cgtctcatcc
     1081 gagacctccg ccgcggcagt cgaaccggcc gccgagcagc agccacagga gagcgctaag
     1141 gtcgcttccg gctcgtcgac gccgcaaggc cgtgcagcaa acgatccacg tgagcggaac
     1201 atagccgatc aaccgacgcc cgcggcgcgt gagaaagcgc cggcttcgga cgcggcgccg
     1261 ccaacgccgc cggctgagcc cgtggcacag gctccggacg taccggcagc gagcccagac
     1321 aagccagcgg ccgaagaacc ggcggtcgcg aaacgacccg aggcaaaagg ccagccgaag
     1381 gatgcctccg tcgagcccgc gcccgaggcg ccaacgccgg agccgcgccc gtcccgggcc
     1441 ttcaacgatc cgagagaagt gcgccgacgt gagcgggaag cggaactcaa agccgagggc
     1501 gtgataccca agaccgggga tcaatccacc ccgtagacct gcggttccag ctccagcgac
     1561 acgccgtata gtcgttgtac ctccgcgcgg atggactggc caaacgccag aacgtccgcg
     1621 ccgctggcgc cgtcgcgatt gatcagaacg agcggctggc gcggccagac gccgactttg
     1681 ccggcggccc gcgccttgaa accggcccga tcaatcagct gcgctgcgga tagcttcacc
     1741 ctgccgtcgc tctggaaatg ctcgaccagg cccggcactg cagcgcgtag accttcggcc
     1801 ttgggcgcgg aaaccaccgg attcttgaag aagctgccgg cattccctac ccggcgaggg
     1861 tcaggcagct tgcggcgacg aacacgcaca accgcttcgg cgaccagcac gggcgagatc
     1921 cgtgagccgc ccagacgctg cagctcgagg cgcagctccg gatagtcgat gaccggctgc
     1981 gcctcacgcg aaagcgcgag ggtcacgtcc agaatgatga aacgaccggc gtcagcagat
     2041 ttgaagcggc tgtcgcgata ggcgaaaccg cactcttccc gatgaaactc tcgaacggcg
     2101 cccgaggcga cttccatggc ccgcaagctg atcagccgat cggccagttc cacgccgtag
     2161 gcgccaatgt tctgcaatgg ggcagcccct acggaacctg gtatcagggc aaggttttcc
     2221 agcccgtaca ggccctggcc aatagtaaaa cgcacgagcc catgccagct ctccccggcc
     2281 gctacggaga cgttcacccg ccctccccgg ccagggacga tgtgcgtgcc acgggtacga
     2341 atgacggcga cggttcccgg aacgtggcgc ttgagaacga cgttgctgcc gccgccgagc
     2401 acgacgagcc cgccccgctg atcggcggct tcacgcagga gcccctgcag cgcccgctcg
     2461 ctctcgacca cgaccaaccg atcggccgtc gcagcgacgc ccagggtatt gagtgcgtcc
     2521 agtcgaggcg ccgcgatcac cgggagacga ccgtgcgaaa cgacaggttg atccgttctc
     2581 ccacgggccg gcgtgtcttc atcagggcgt gaggaaggtg ccgctcgtgg caaagcagcg
     2641 ccccgtggct caagtccagc gcaatgcttc cggtcccgcc gtccacccgg actcgcagcc
     2701 gacgagtcgc gccgaggctc agagacgcga tcggccctgc cagctcgggc tcgtcatccc
     2761 ggtgccaacc catggagtcg gagccgtcgc gatatcgatt gatcagcacg aagttgaagc
     2821 ccgccgacca ttcgcgccgc aggagatcgc gcaccgagca aagctgagag ggccagcccc
     2881 cggcaacgtg ttcaacgccc gaataccggt agcagacccc ggggtctccg taccaggcaa
     2941 cgagccttgg caccgccact gacttcccga gtattctgat ccgctcgctt cgccagtcga
     3001 cgctttcgcg cagccagccg agcaggacgt ctgccggggt atcagcgagg tatgacggga
     3061 aatggcgata gcctgcttgg ggtatactcg cgcgctcgtt tgtggaggga agaacagtgg
     3121 atctgaacgg caaagtggct gtcatcaccg gtggggcatc cggcctcggg cgcgcgacgg
     3181 cggagatggt aatcaacaat ggcggcaaag tcgccttgtt cgacctcaac gaggaccttg
     3241 cccgtcagac cgccgccgcg ctcggcgacg acgctgcacc ctttgccgtc gacgtgacga
     3301 acgacgaatc cgccgctgcg gccgtcgacc aggttctgga aagattcggc gcgattcacg
     3361 tgaacgtgaa ctgcgcaggc atcggtgctg cgggccggac catctccaaa gaaggtgcga
     3421 tgcccttgaa gcacttcaat ttcatcattc aggtgaatct cgtcggcacg ttcaacaccc
     3481 tgaggctttg cgcggccgcg atgaacgaga acgagccgac cacggaggac ggcgagaaag
     3541 gcgtcatcat caataccgcc tcggtggcag cgttcgatgg ccagatcggt caggcagcgt
     3601 actccgcgag caaggccggc gtcgccggca tgaccttacc catcgccagg gacctggcca
     3661 gatccggtat ccgggtctgc accatcgccc ccggcatctt cgaaacgccg atgatggctg
     3721 gcgcaccgga ccgagtcagg gatccgctca tcgagatgac tcagtttcca aaacgcctcg
     3781 gcaacgcggc ggagtacgca ttgctcgccc ggcagatcat cgagaacacc tacttgaacg
     3841 gcgaaaccat tcggctggac ggaggaatcc ggatgtcgcc ccgctaagag cgcctcacga
     3901 tggggatcgg gttcggactc tttccaggtc cgccggcgtg tccacgccgg ccggaaccgt
     3961 ttcgcaggcc tctgtgacga agatcggaat gccgttttcc agcaggcgca gctgctccag
     4021 cttctccaag cgctcgaggt ctgacacggg cagctcgaca aactgctgca gggctgagag
     4081 gcgatatccg tagacgccga tgtggcgcag gccgatcgga cgccctcgat cctgtccgga
     4141 tttccctggc gcttcgtcac gctcgtaagg tatcggggcg cgggaaaagt acagcgctcg
     4201 cccccaccgg tcccgcacca ccttgacgac gttgggatcg gtcaagaccg ccacctccct
     4261 gatcggctcg cacagcgtgg ccaccggacg ggccgggtct gcctgaagga tctgcgcgac
     4321 ctgcatgacg acggcgggtg gcatctgggg ctcgtcaccc tgaacgttga tcacgatggc
     4381 atcgtcgtcc caggcccgca agcgggcgac ctccatgacc cggtcggaac ccgacgcgtg
     4441 atcggcgcgg gtcatggtcg cttcgaaacc cgcggaagtc accgcttcca cgattcggac
     4501 atcgtccgtg gcaatcacca cttcctccgc gccggaggcc gcgcagcgtt cggccacccg
     4561 cacgatcatc ggcttgccgt gcagatctgc cagaggcttt ccgggaagtc tggacgagcc
     4621 gtaccgggcg ggcaccacga ccttgaacgg ctcagccatg gcggctgatc ccgtgggctc
     4681 tcaacagctc ctctaagaac gcagcggcgc cgtcgggcag atgcgcctcc acttcgaggt
     4741 accagacatc ggccggcagg tcggtcaggc gccgaatctt caccgcatcc ttttcggtgc
     4801 agaccaccgg ccagtcgttg tcgaagacta gctcgctgcc gtcgtaggga tgatgatcgg
     4861 gctgggcatg aagaaccggg gcaagcccca gctcgcgcaa cgccagcgca aatctggcgg
     4921 gattgcccac accggcgacg gcgttgacgt tcggatgagc ggccacgaaa tcctccggac
     4981 gctggcgtgc ctctcccggt cccggcgtga ctcgaacgaa agcggtgggg atcagcgtca
     5041 tgacggtctc tcgggccacg atcccggatc ccaggccatt gctgatcacc cagtcgacgc
     5101 tctcgagccg gctgacgggc tcgcgtagcg gcccggcggg aaggcagcgg ccattgccga
     5161 cgcctcgatg accatcgatc agcgcgattt cgagatccct cgccagggcg tagtgctgca
     5221 gcccgtcgtc cgagacgacc acgtcgctgt ttgccgcctg cagaagactc tctaccgccc
     5281 tggcacgatc ggctgccacc accaccgggc agcgggcccg gctcgcgatc atgggcgctt
     5341 cgtcaccgac gacggcgggg tccgaatccg gggtcaccag caggggcttg cgtccccgtt
     5401 taccgccgta acctctggag acgactcccg gcgagtaccc gagcgccctc aactgctcga
     5461 ccaaccagat aaccatgggc gtcttgcccg taccacccgc ggtgatgttt cccaccacga
     5521 tcacgggaac cgggctgcgc caggagcggc gcttgccggt aagaaagcgc aacctgcgac
     5581 gcaccgcgta gcgttcgaac gcccatgcga aaggcgcgag cagcaccgtc cagcggctgc
     5641 ccgtgtacca ggcctgggcc agggggcgca gggtctggcc caccgtcagc ttgccggtca
     5701 ggcccggagc cgtggacccg gtgggtgcct cggccaccgc agcggaatcc ggctcgtcgt
     5761 cgaactgggc ctgatagagc gcggcgtaat gtccaccccg gttcagcagc gcctggtgag
     5821 tacccgcctc tacgatcctg ccttcatcaa cgacgagaat ctcgtcggcg ttctcgatgg
     5881 tggacagacg atgggcaatg acgagcgtgg tgcgtccgcg catcacctca tccagcgccg
     5941 cccggatatg ccgttcggac tccgtgtcca gagccgacgt tgcctcgtcc agaatgagca
     6001 ccggcgcgtc cttgagcagg gccctggcga tggcgacccg ctggcgctga cctcccgaaa
     6061 gcagaacgcc gtcgtcgccc accaccgtct gcagtccgtc cggaagctcg gagacgaacc
     6121 gatccgcatg agcccgcctc accgcgcgat cgacctcgtc cggatcagcc ccggccaggg
     6181 ctccgtaggc tatgttgcgc tcgagggtat cgttgaagag cgtcacctgc tgggtcacaa
     6241 gggctatctg gctgcgcaga gcttccaacc ggtagcgcgg aagcggcacc ccgtccagga
     6301 gaatctcgcc ccctgtcggc tcgtaaaacc ggggtatcag gctcgcgatg gtggttttgc
     6361 cgctgccgga gcgacccacc aaagccacgg tttttcccgg ctcgacggta aagctgacgt
     6421 ggctgagcac cggtgcgctg ccgcgttcat aggtaaagct gacgtcgcga aattccagtc
     6481 gacccgcgac gcgttcggtg gtcatgctac ccagatcgct ctcggtgtcg gcatccatct
     6541 gcccgaaaac gtcctccgct gcggcaagac cacgctgcag tcgggcgttc acctccgaga
     6601 gtttgcggat cggccttgcg agcagcccgc tcaagcccag aaaggtgacc acctgacccg
     6661 tgctgagggt tccgccgatc tccgggcgga acagcaggcc gatgagcagc gccagggcca
     6721 cggcgacaag cacctggatt gcctgagtgc tggaaacctt ggtccccacc attttcagat
     6781 tctggcgacg attggaccgg ctcgatttgt ggaaccgggc ctgctcgtag gcttccccgc
     6841 cgaagatccg cacgacgcgg tacccgctaa cggcttcgga agccacgtga gtgacgtcgc
     6901 ccatggagtt ctgaatgcgc cggcttatcc gcctgaaacg ccggctggcg tagacggcca
     6961 ccaggccgac cagcggagcc gcggcgacga agatcagcgt cagcttccag ctcaggtaga
     7021 acatgtaacc caggtagacg atgaccttcc cgccatcctg gatgatcgtt ttgagcgcgt
     7081 cggtacccgc atcgcgcagc tgcgccacgt tgtaagtgag gcgcgagacc aggtgtccct
     7141 gagagctggc atcgaaatac gcgctgggca tgagcagaag ccggtcgaac agcgccgtgc
     7201 ggatgttgtg aacgacgccg aacgaaatct ggctgagcag catctcgccg atcacctcgc
     7261 cgctgccgcg aacgagggcg accacgcaca tggttgccgg aatggcccag ctggcgtgca
     7321 cgacgatgtc gtcccagttg tcgatcaggt caccgaagag cttgacgaag taagcctcgg
     7381 cggcgctgcc gacgagaaag ccgaggatgg ccagcagaaa cacgaaccag tagggacgta
     7441 cgtagccgag cagccgccga tacgtgctcc agtctccgcc ggacggaggg ctatccgccg
     7501 gggcgggcgc ttcgtcgctc atctgcgcgc gccacgccgg ctgggcacga ctactgctcc
     7561 tcctccggct gcttggtggt gaggctgatc tgggtcagac ccacccggcc agcggcatcc
     7621 atggctcgga cgaccgcctg atgagaggct tgcgcgtcgg ccgtgacgat cacgcgccgc
     7681 gattcgcccc gctcggcgac catttcctcg agggcccgga tcagcgtgcg gagctccgcg
     7741 ttgaccagaa gccggtcgtt gaccgcgtac tcgccacgcc tgtcgatggt gatttctatg
     7801 aaatctctct ccacctcctg aatctcgcca gatgcttccg gaaggttgat cttcagctgc
     7861 gtctcgcgaa tgaacgtcgt cgacaccatg aagaaaatga gcagcagaaa caccacgtcg
     7921 atgagcggcg tcagctcgac ggtggcttcg ggttgtcggc gtcgcgtgaa tttcatcgac
     7981 ggcaaacccc gttagagccg cggatcatca tccggcccgc cctgcatgat ctccaccagc
     8041 ttgcccgcct cctgctccat ggtcaccatg agctcgtcca cccgccgcag aaagaaacgg
     8101 tggaacatga gtgcaggaat ggcgacggag agaccggcgg cggtcgtgat cagcgcctgg
     8161 gaaatgcctc cggcgagcac gttggcgttg ccggcccctt ccagcatgag ggcggtgaaa
     8221 accttgatca tacccaccac ggtcccaagc aggcccagca gcggcgagat ggacgcaatg
     8281 accccgagcg aggtgaggta gcgctccatg tcgtggctca cctgtgcggc cgactcctcc
     8341 atggcttctt tcatgatctc ccggccgcgc ttggcattgc cgatacctgc ggcgagcacc
     8401 tgcccgagcg ggctcgaggc ccgaatgtcc ttcagacgct gagcgtccag ccggtcgccc
     8461 ttgatggcat cccagacctc agctaagaga tcggttggag cgatgcgccc acgctgaagc
     8521 gtccagagcc gctctacgct gatcgcagcc gcaatgatgc tgcacaacag gatcggcacc
     8581 atcagccagc caccggattg aacgatctct aacacgccgt agcctccatt gctatttttc
     8641 gcgcccctac caggagcatc cgcgcgccgc gacgggccat atgctatgcg ccaatccatc
     8701 ccggtgcacc cagtagtggt cgtagaggtt cacagtcgga tcacaatgtg aggttatgaa
     8761 ctgatgaacc tctccgagca ggggctcctg gcttcccgcc gccgggatga cgacgccgtg
     8821 ctcgtctccg gcaaaccggt accgcgcacc cgcaatgtgc atgggctcgg gcgccacggt
     8881 atccgaggcg aacgccttgt agcccgcatc caccgtcacc gcgctgtcca acggtttgct
     8941 gatggccgtg gcagcgacgg tcaatgccac ctcgaagtcg tcgaagacct cgccccgggc
     9001 accgccaatg acccgatatt cgtgatccat gaacacatag ctgcccacct gaacgtcggt
     9061 gacagcctcg atgtccgtgt cgatgtcgta agtcccggta ccgccgccgg tgaccactgc
     9121 cggggcaagg ccgttttggc gaaggcgatc gacgaccccg gcgacctgct cccagagcgc
     9181 cgtcgacctc cggcgtcggt tctggtggcc ttcgacgtgc atcagatgcc ccgcatagtg
     9241 ctgcacaccc tcgtagcgca ggcccggggt cgtctcgatg ctctccgcga gcgcgagcac
     9301 ctgatcagga tcccgacagc ccgtgcgccc catgtccaca tcgatgtcca ccagcacgcc
     9361 cacctctgca tccggacagg ctgcctctct aagcacctgc agtgccaacg ggccgtctac
     9421 caccacgctc acacggcctt tccg
//
LOCUS       DBNHLM010000282         7656 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1418396, whole genome shotgun sequence.
ACCESSION   DBNHLM010000282 DBNHLM010000000
VERSION     DBNHLM010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7656)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7656
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1418396"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="AC16QF_13065"
     CDS             complement(<1..891)
                     /locus_tag="AC16QF_13065"
                     /inference="COORDINATES: protein motif:HMM:NF014826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR6449536.1"
                     /translation="MATSPDKPYDGGFAELHCISNYSFLRGASHPEELVKRATELGYR
                     ALALTDECTYAGLVKAHVAARELGIKLIVGAEFRLEEGIHLILLAPDRSAYGQLSALI
                     TKLRRRSPKGAYEVCLDDFRWGVDECLALWVAGDGDIETLMAQGRRLSERWRELWIAL
                     ELFREDKDLDKMANALTLSMRLDLPIVASNDVHAHLPARQPLQDVLTAIRLKTTVAEL
                     GCAAHRNGERHLRSLSELVSLYPPEMLEASCQIADRCRFSMDELRYEYPEELVSPHLT
                     PGEYLRQLTLAGAAERWPDGV"
     gene            complement(875..2290)
                     /locus_tag="AC16QF_13070"
     CDS             complement(875..2290)
                     /locus_tag="AC16QF_13070"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIR6449537.1"
                     /translation="MLWLGLHLPNLGLEIFEAAAPGTPQVLVEDNRVILQNPAAVAAG
                     ITPGSTLATAHSIEPRLRHCNRDEIRERERLELLAGALYGFSSHVSTQPPSSLVLEAG
                     GSLELFGDLAKLEQAVRETCEALGHVTIMRTALTPSAAMTLARARQDDLKHVPLELTE
                     LATSMVERFANMGMHALGALIALPEDELAHRFGPELVNYLKRLTGRMPDPREAVTPAE
                     AFSSRLHLLEAVTGKDALLFPMQRLLGELQIWLVSRQLGAEQIVWTLDDHGSKDTAEL
                     TIRFARAQQRREAFLDITRLKLESAELPGDVLGIGLRAERLVPWQAGSRTLFQSQPGQ
                     APADAAGLVDQLCARLGNRACHGIRPVDQHAPEQAWRLSRPAMNGRGSRGKQGNDRMT
                     DGKEALLPAPDERPIWLLSRPRPINRSELKLLKGPERIRTAWWQRGISRDYYVARDRR
                     GAECWAFVDAGNNWYLHGYFA"
     gene            complement(2290..3084)
                     /gene="imuA"
                     /locus_tag="AC16QF_13075"
     CDS             complement(2290..3084)
                     /gene="imuA"
                     /locus_tag="AC16QF_13075"
                     /inference="COORDINATES: protein motif:HMM:NF033429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HIR6449538.1"
                     /translation="MAEPSGKASRLLEHPALWRAGQLNQATEALSTGFPELDAHLPGR
                     GWPRAGLSEFLLDTTGAGELKLLAPLLKDLSRSEARWIAWVNPPFIPYAPALTALGID
                     VDRVLLIHPRDHKDALWAFERAARSGGCSVVLGWLDEKQLKVKDTRRLQLAARQGRAF
                     SCLFRPVSAAAESSMAELRLKMKALGSGKLGVSITKRRGGWPVTGLNLTVAEPRRPAE
                     IREQLSLWRSLQHPQDAGNEAELPVLGNPAPAGATHAPSSPGRLVH"
     gene            complement(3167..4504)
                     /locus_tag="AC16QF_13080"
     CDS             complement(3167..4504)
                     /locus_tag="AC16QF_13080"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007352049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose dehydrogenase family protein"
                     /protein_id="HIR6449539.1"
                     /translation="MKITMFGTGYVGLVTGTCFAEMGNEVLCVDVNAEKIEALNAGQI
                     PIYEPGLEQMVKSNASGGRLKFSTDVEEGVHHGRVLFIAVGTPSDEDGSADLSYVIGV
                     AKTIGQHMAEPKVVVDKSTVPVGTADQVRAAIGETLAARGAEIDFDVVSNPEFLKEGA
                     AINDFMKPDRIVIGATNPETIATMEALYAPFNRNHDKIVHMDVRSAELTKYAANVMLA
                     TRISLMNEIANLADLLGADIEAVRRGIGTDPRIGYHFIYAGSGYGGSCFPKDVKALIH
                     TASEAGYDAAIVRGVEAVNERQKSVLVGKLRRHFDDDLEGRTFALWGLAFKPNTDDMR
                     EAPSCTIMEQLFAAGARVRAYDPAAMPEAAHLYDGQDGLTLCEARDGTLEGADALVVV
                     TEWNEFRSPDFLDVKARLKQPVIFDGRNLYDPDYLAQLGFDYYAIGRGKPSQT"
     gene            complement(4514..6058)
                     /locus_tag="AC16QF_13085"
     CDS             complement(4514..6058)
                     /locus_tag="AC16QF_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HIR6449540.1"
                     /translation="MNGAQSLLKTFVNAGVEVCFTNPGTSEMHFVAALDTEDGMRCVL
                     GLFEGVVTGAADGYARMAGKPACTLLHLGPGLANGLANLHNARRANSPIVNVVGEHAT
                     YHRHLDAPLTSDIEGFASPVSGWTRVSQSAASVAADGAEALRASLQPPGQIATLILPA
                     DTAWNEPAAEADPLPSADPRPFDALAVEAAARALRSGEPCALLLNGVGVSEAGQILAS
                     RIAEHCGARLLGDTFIARIPGGAGRVTIPRLPYFAEQAEEALAGVRHLIMVDTKPPVS
                     FFAYPGRASELTPNGAEAIVLTQAGEDTIGALEALVDAVGATGQQPVLADLQRPDLPD
                     GPLNPETIARALAALLPEQAILVNEAATSGFGLPAFLPRTAPYDMLDLTGGAIGMGLP
                     AATGAAIACPDRRVVALQADGSAMYTVQALWTMAREGLDVTVVLFSNRKYAILQVEFM
                     RVGAHNPGPKAMDMLDLTRPDLDWVSIARGMGVDAVRADDAASFNQALGRSFATPGPF
                     LIEAVI"
     gene            6189..7541
                     /gene="gorA"
                     /locus_tag="AC16QF_13090"
     CDS             6189..7541
                     /gene="gorA"
                     /locus_tag="AC16QF_13090"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016561825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HIR6449541.1"
                     /translation="MSYDYDLVVIGAGSGGVRAARMSAGYGARVAVAESTYLGGTCVN
                     VGCVPKKLFVYGSHFHEDFEDAAAYGWDVSPTGFDWPRLRDNKTREIERLNGVYANLL
                     EKAGVETKWGHARIAGPNTVAMDGQTFTAEHILIATGSWPAVPDVPGRELAITSNEAF
                     YLESFPKRVVVVGGGYIAVEFAGIFKGLGANVELLYRRDLFLRGFDDGVRQFVANELT
                     QKDIGLRFNTNVARIEAVDGRRRVHLVEGGSLDADLVLYATGRTPKTGDLGLETVDVA
                     LNERGGIVVDEFYRTSVPGIYALGDVIDNVQLTPVAIAEGMCVSANLFTDRPASRVNY
                     ENIPTAVFCQPNIGTVGPTETEARERYPDLAVFESEFRPMKHTLTGRQERTFMKLLVD
                     GASDRVVGAHMVGPEAGEIVQGIGVAITAGATKAHFDQTIGIHPTAAEEFVTMREPAR
                     "
BASE COUNT         1216 a         2475 c         2669 g         1296 t
ORIGIN      
        1 cacgccgtcc ggccagcgtt cggccgcgcc ggcgagggtg agctgtctta agtactcgcc
       61 cggagtcagg tggggcgaca ccagctcttc gggatactcg tagcgaagct cgtccatgga
      121 gaagcggcag cggtcggcga tctggcagga cgcctcgagc atttccggtg ggtagagaga
      181 gacgagctcg ctcagggagc gcaggtggcg ctcgccgttg cgatgggccg cgcagccgag
      241 ctcggcgacc gtggtcttca ggcgaatggc ggtgaggacg tcctgcaggg gctgccgggc
      301 ggggaggtgc gcgtggacgt cgttgctggc cacgatgggc aggtccaggc gcatggacag
      361 ggtcagggcg ttggccatct tgtcgaggtc cttatcctcc cggaagagtt cgagagcaat
      421 ccagagctcg cgccagcgtt cgctgagccg tctgccctgg gccatgaggg tttcgatgtc
      481 gccgtcgccg gctacccaca gcgcaaggca ttcatccacg ccccagcgga agtcgtccag
      541 gcagacctcg taggcgccct tgggggagcg tctgcgcagc ttggtgatca gcgcggagag
      601 ctgcccgtag gcgctgcggt ccggcgccag caggatgagg tgaatgcctt cctcgaggcg
      661 gaactccgcg cccacgatga gcttgatgcc gagctcgcgc gccgctacgt gagccttgac
      721 gagtcccgca taggtgcact cgtcggtcag cgcaagcgcg cggtagccga gctcggtggc
      781 ccgtttgacg agctcctccg gatgggaggc tccgcgcaga aagctgtagt tgctgatgca
      841 gtgcagctcc gcgaagccac cgtcgtacgg cttgtcaggc gaagtagcca tgcaggtacc
      901 agttgttccc cgcgtcgacg aaggcccagc attcagcgcc ccggcggtcc cgggcgacgt
      961 agtaatcccg gctgatgccg cgctgccacc aggcagtgcg gatacgctcg gggcccttga
     1021 gcagtttcag ctcgctcctg ttgatcgggc gcggccggct gagcagccag atcggccgct
     1081 cgtcgggcgc gggcaggagc gcttccttcc cgtctgtcat cctgtcgttg ccctgcttgc
     1141 cgcggctccc gcgcccgttc atggcaggtc tcgagaggcg ccaggcctgc tccggcgcgt
     1201 gctggtccac cgggcggatg ccgtggcagg cccggttgcc cagccgggcg caaagctggt
     1261 cgacgagccc ggcagcgtct gcgggggcct gtccgggctg ggattgaaaa agtgtccggc
     1321 tgccggcctg ccagggcacc aggcgctcgg cacgcaggcc gatgcccagc acgtcgccgg
     1381 gcaattccgc gctttcgagc ttcagccggg tgatgtcgag aaaggcctcc cgccgctgct
     1441 gggccctggc aaagcggatt gtcagctcgg ccgtgtcctt cgatccgtga tcgtcgagcg
     1501 tccagacgat ctgctcggca cccagctgcc ggctcaccag ccagatctgc agctcgccga
     1561 gcagccgctg catggggaag agcagggcgt ccttgcccgt caccgcctcc agcaggtgca
     1621 gccggctaga gaacgcctct gccggcgtca cggcctcccg gggatccggc atccggccgg
     1681 tcagccgttt gagatagttc acgagctccg ggccgaaccg atgggcgagc tcgtcctccg
     1741 gcagggcgat cagcgctccc agggcgtgca tgcccatgtt ggcgaagcgc tccaccatcg
     1801 acgtggccag ctccgtgagc tccaggggga cgtgtttcag atcgtcctgt cgggcacggg
     1861 ctaaggtcat ggccgccgag ggggtcaggg ccgttcgcat gatcgtgacg tgaccgagag
     1921 cttcgcaggt ctcgcggact gcctgctcca gtttggccag atcgccgaac agctccaggc
     1981 tgccgcctgc ctccagcacc agggagctcg gcggctgggt ggagacgtga gaggagaaac
     2041 cgtagagcgc tccggccagg agctccagcc gctcccgttc ccgaatctcg tcccgattgc
     2101 agtgccgcag ccgcggctcg atgctgtgag cggtggccag ggtgctgcct ggggtgatgc
     2161 cggcggcgac cgcggccggg ttctgcagga tgacccggtt gtcctccacc agcacctggg
     2221 gcgtgccggg cgctgctgct tcgaagattt cgagccccag gttggggagg tgcaggccga
     2281 gccagagcat cagtggacga ggcggccggg ggaagagggc gcgtgggttg cacccgccgg
     2341 cgccgggttg ccgaggacgg gcagctccgc ctcgttaccg gcgtcctgcg gatgctgcag
     2401 gctgcgccag agcgacagct gctcgcggat ctctgcgggc cggcggggtt cggccacggt
     2461 caggttcagc cccgtgaccg gccagcctcc gcgccgtttg gtgatcgaca ctcccagctt
     2521 tccggatccg agagccttca tcttcagcct cagctcggcc atggagctct ccgccgcggc
     2581 gctcaccggg cggaacaggc aggagaacgc acgcccctgc cgggcggcga gctgcagcct
     2641 ccgggtgtct ttgaccttta gctgcttctc gtcgagccag cccagcacca cgctgcaccc
     2701 gcccgaccgc gccgcccgct cgaaggccca cagagcgtcc ttgtggtccc gcggatggat
     2761 gagcagcacc ctgtccacgt cgatgccgag cgcagtgagg gccggggcgt aagggatgaa
     2821 cggcgggttg acccaggcga tccagcgcgc ctcgctgcgg ctcaagtcct tgagcagagg
     2881 tgcgagcagc ttcagctcgc cggcgcccgt ggtgtccagg agaaactcgc tcaggcccgc
     2941 tctcggccag ccccggccgg gcagatgagc atcgagctcg ggaaaacccg tgctgagcgc
     3001 ctcggttgcc tggttcagct gcccggcacg ccagagtgcg gggtgttcaa gaagtcggga
     3061 ggcttttccg gacggctcgg ccatgctcgc gctcgatacg gtcttttcat acaggggtcg
     3121 gccagattac ctgtatggat atacagtatc aagagattcc ggagcgtcag gtctggctcg
     3181 gcttgccccg tccgatggcg tagtagtcga agcccagctg ggcgagatag tccgggtcgt
     3241 agaggttgcg gccatcgaag atcacgggct gcttgagccg cgccttgacg tccaggaagt
     3301 cggggctgcg gaactcgttc cactccgtga ccacgaccag tgcgtccgcg ccttccaggg
     3361 tgccgtcccg cgcttcgcac agggtgaggc catcctgccc gtcgtaaaga tgcgcggctt
     3421 ccggcatggc ggcggggtcg taggccctca ccctggcacc ggctgcaaac agctgctcca
     3481 tgatggtgca gctcggcgcc tcacgcatgt cgtcagtgtt gggcttgaag gcgaggcccc
     3541 acagggcgaa cgtgcgtcct tcgaggtcgt cgtcgaaatg cctgcgcagc ttgcccacca
     3601 gcacgctctt ctggcgctcg ttcactgctt ccacgccgcg gacgatggcc gcgtcgtagc
     3661 ccgcctcgct ggccgtgtgg atcagcgcct tcacgtcctt tggaaagcag gagccgccat
     3721 agccgctgcc cgcgtagatg aagtggtagc cgatgcgcgg atccgtgccg atgccgcggc
     3781 gcaccgcttc gatgtcggca ccgagcaggt cggccaggtt ggcgatctcg ttcatgaggc
     3841 tgatgcgcgt ggccagcatc acgttcgccg cgtacttggt gagctccgcc gagcgcacgt
     3901 ccatgtggac gatcttgtca tggttgcggt tgaagggagc gtacagcgcc tccatggtcg
     3961 caatcgtctc gggattggtg gcgccgatca cgatgcggtc cggcttcatg aagtcgttga
     4021 tggccgcgcc ttccttgagg aactcgggat tggagaccac gtcgaagtcg atctccgcac
     4081 cccgggccgc aagggtttcc ccgatggcgg cgcgaacctg atccgccgtg cccacgggca
     4141 cggtggattt gtcgaccacc accttgggct cggccatgtg ctgcccgatg gtcttcgcca
     4201 cgccgatcac gtagctcaag tccgccgagc cgtcctcgtc cgaaggcgtc ccaacggcga
     4261 tgaagagaac ccggccgtgg tgcacgcctt cctcgacgtc tgtgctgaac ttcagacggc
     4321 caccggaggc gttggacttg accatctgct cgagcccggg ctcgtagatg ggaatctgtc
     4381 cagcgttgag ggcttcgatc ttctccgcgt tcacgtccac gcagaggacc tcgttgccca
     4441 tctcggcaaa gcaggtaccg gtgaccaggc ccacgtagcc ggtcccgaac atggtgatct
     4501 tcatgatttc attctaaatg acggcttcga tgagaaacgg gcccggggtc gcaaagctgc
     4561 gcccgagcgc ctggttgaac gacgccgcat cgtcagcgcg aacggcgtct acccccatcc
     4621 cccgggcaat gctcacccag tcgaggtccg gtcgggtgag atccagcatg tccatggctt
     4681 tcggcccggg attgtgagcg cccacgcgca tgaactccac ctgcaggatg gcgtacttgc
     4741 ggtttgagaa cagaaccacc gtcacatcca gcccctcccg tgccatggtc cacagagcct
     4801 gcactgtgta catggcactg ccgtctgcct gcagcgccac gacccgccgg tctggacagg
     4861 cgatggcggc accggtagcg gcgggcagcc ccatgccgat ggcgccgccg gtcaggtcga
     4921 gcatgtcgta gggggccgtg cgaggcagaa acgccgggag cccgaacccg gaggtggccg
     4981 cctcgttgac gaggatggcc tgctcgggta gcagggcggc aagagcccgg gcgatggtct
     5041 cggggttcag cgggccgtcg gggagatcgg gccgttgcag atcggcaagc acgggctgct
     5101 ggccggtcgc ccccacggca tcgaccaggg cctccagcgc gccgatggtg tcttctcccg
     5161 cctgggtgag cacgatggcc tccgccccat tgggagtgag ctcgctggcg cgacccgggt
     5221 aggcgaaaaa gctcaccggt ggtttcgtgt ccaccatgat caggtggcgg acgcctgcca
     5281 gagcctcttc ggcctgctcg gcaaagtagg gaagacgcgg gatggtgacc cggccggcgc
     5341 ccccggggat ccgcgcgatg aaggtatctc ccagcagccg cgcgccgcag tgctcggcga
     5401 tgcggctggc caggatctgt cccgcctcgc tcacgcccac gccgttcaag agcagcgcgc
     5461 agggctcgcc ggagcgcaac gcccgggcgg ccgcttccac ggcgagggca tcgaatggtc
     5521 tggggtctgc cgaaggaaga ggatcggcct cggccgcagg ttcgttccag gccgtgtcgg
     5581 cgggcaggat gagggtggcg atctgccccg gcggctgcag cgaagcccgc agcgcctcgg
     5641 cgccatcggc ggctaccgat gcggctgact gcgagacgcg cgtccagccg gaaaccgggc
     5701 tcgcgaagcc ttcgatgtcg gaggtcagcg gcgcgtccag atgccggtgg taggttgcgt
     5761 gctcacccac cacgttgacg attggagagt tcgcccggcg ggcgttgtgc agattggcga
     5821 ggccgttggc caggcccggg cccaggtgca gcagcgtgca tgccggcttg ccggccatgc
     5881 gggcgtaccc gtccgccgct ccggtgacca ctccctcgaa cagcccgagc acgcagcgca
     5941 tgccgtcctc cgtgtccagc gcggcaacga agtgcatctc cgaggttccc ggattggtga
     6001 agcagacttc cacgcctgcg ttcacgaacg tcttcaacaa gctctgcgcg ccgttcatgg
     6061 ccgtaccggt gacgagagga aagcggagtg tatgtgggcc ggaagaagca ttgcaattcg
     6121 gtgctcatca attacgctcc gtcacatccc gcatctgcgg gtccgtttcc ccggggagaa
     6181 tcaagacgat gagctacgac tacgacctgg tcgtgatcgg cgcgggatcc ggcggcgtca
     6241 gagccgcgcg gatgtcggcc ggatacggcg cccgggtggc cgtagccgag agcacctacc
     6301 tgggcggcac gtgcgtcaac gtgggctgcg tgcccaagaa gctcttcgtc tacggctccc
     6361 actttcacga ggatttcgag gacgccgcgg cgtacggctg ggacgtttcg cccacgggat
     6421 tcgactggcc gaggctgcgc gacaacaaga cgcgggagat cgagcggctc aacggcgtgt
     6481 acgcgaacct gctcgagaag gcgggcgtgg aaaccaaatg gggccatgcg cgaatcgcag
     6541 ggccgaacac ggtggccatg gacgggcaga cgttcacggc cgagcacatt ctgatcgcca
     6601 caggcagctg gcccgcggtg cccgacgtcc ccggccggga gctggccatc acctccaacg
     6661 aagcgttcta tctggagtcg tttcccaaac gcgtggtggt ggtcggcggc ggctacatcg
     6721 cggtggagtt tgccggaata ttcaaaggcc tcggggcgaa cgtcgagctg ctctaccggc
     6781 gcgatctgtt cctgcggggc ttcgacgacg gggtgcgtca gttcgtcgcc aacgagctca
     6841 cccagaagga cattggtctg aggttcaaca ccaacgtggc ccgcatcgaa gcggtggacg
     6901 gccggcggcg cgtgcacctg gtggagggtg gatctctcga cgcggatctg gtgctctacg
     6961 ctaccggcag gaccccgaag acgggcgatc tgggcctgga aacggtagat gttgcgctca
     7021 acgagcgtgg cggcatcgtg gtggacgagt tctaccggac ttcggtgccc ggcatctatg
     7081 cgcttggcga cgtgatcgac aacgtgcagc tgacgccggt agccatcgcc gaggggatgt
     7141 gcgtgtctgc caacctgttt accgaccggc cggccagccg cgtgaattac gagaacattc
     7201 ccaccgccgt gttctgccag ccgaacatcg gcacggtggg gccgacggag accgaagccc
     7261 gggagcgcta cccggacctt gccgtgttcg agtcggagtt ccggcccatg aagcacacgc
     7321 tgaccggccg gcaggagcgc acgttcatga agcttctggt cgacggtgcg agtgaccgcg
     7381 tcgtcggtgc gcacatggtc ggtccggaag ctggcgagat cgtccagggc atcggcgtcg
     7441 ccatcacggc gggtgcgacc aaggcgcact tcgaccagac catcggcatt caccccacgg
     7501 cggcggagga gttcgtcacc atgcgcgagc ccgcaaggta gccgcgggtg cagcaagggg
     7561 agcggacggc atgaagagga tgacaagttt ccggacgcga tggccgagat ggcttgcggc
     7621 tgcgtcgctt acggggctga cggcctgcgc gatcat
//
LOCUS       DBNHLM010000283         5911 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_102117, whole genome shotgun sequence.
ACCESSION   DBNHLM010000283 DBNHLM010000000
VERSION     DBNHLM010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_102117"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="AC16QF_13095"
     CDS             complement(<1..398)
                     /locus_tag="AC16QF_13095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6449542.1"
                     /translation="MRFSLMLPVFVDAGAASPFERTLGFARTADELGYHMGTFGHHSF
                     TPQIADPSAPFALLSAVAARTERLRLGTGVYLAPLHHPVTVAEQAAMLDQISGGRAVL
                     GVGLGYRPYEYEGFGLDFHTRGKRLDELLTA"
     gene            complement(416..1027)
                     /locus_tag="AC16QF_13100"
     CDS             complement(416..1027)
                     /locus_tag="AC16QF_13100"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6449543.1"
                     /translation="MKFYNSIGPNPQVVRTFAAERGIELDMEEVDLMAADNRREPYLS
                     KNPAGQSPCLELDNGDYVSEVTAICEYLDETSPGTSLIGSTPEERAETRMWVRRIDLN
                     ICEPLGYGFRFGEGLDFFKDRITTLPEASEGMKRIAAEKIAWLDGLMAGREFVCGDRI
                     TLADVLLYCFLAFGKTVGQPYAETNQNIHAWFERMSQRPSVSA"
     gene            1277..2737
                     /locus_tag="AC16QF_13105"
     CDS             1277..2737
                     /locus_tag="AC16QF_13105"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6449544.1"
                     /translation="MTKGYQVRLPTKIVYGLGSVSDGVKNTTFNVFLLFFYTQVAGLS
                     GSLAGAAILIALVFDAVSDPLIGNISDNFHSRWGRRHPFMYAAALPMAVFFGLIFSPP
                     QGLGQAGLFAWMLTMAIGGRVSRTVYAFPSGALVAEMTSHYDERTSLVSFRVLSGWLG
                     GLVASQMGYLVFFAPSAAYADGRLDPQAYGDYAVFGAITIFCAILICAIGTHRLIPEL
                     KSPPAHTPFTLRRFGVELREVFSNRPYLVLVFTVLVVSTATGFTDVMGLYVNTYFWEF
                     TTAQLAIIVFGALAGVVAAFSVTATLAQRTDKRTMALAMLLVVVIIGPLPVVLRLLGL
                     MPANGDPLLLGIMIAWTGVIVFVAVGVTILAGSMIADTVDLNELRTGKRQEGMFSAAM
                     GLTAKATSGIGGFVGGVVLDVVDFPTGVAAGEVDADTLFALGVAVGPGMFLLWITVLI
                     ILSRYRLSRADHADILGKLEARRNAAEQPGGEAAGG"
     gene            2811..4151
                     /locus_tag="AC16QF_13110"
     CDS             2811..4151
                     /locus_tag="AC16QF_13110"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6449545.1"
                     /translation="MSEAVDARSPGTTYQELLDTDSHAVPDVLRLESPAPRRLNDLSI
                     DRYTSRDFHDREMEKLWSRVWQFACREEHIPNPGDYLVYEIGRHSYVVVRSHQGAIKC
                     FVNACLHRGRMLKHYDGNCTELRCPFHGFAWETDGRLKDIPASWDFPQIVASEFSLPE
                     AQVATWAGFVFVNPDPDAAPFREFLGGMAAHFDRWNLADRYVQVHVIKEMRANWKIAQ
                     EAFCEAFHVNATHPQTMPYLGDTNSQVDVWDTFSRVITPGGTPSPLLDWEPDESDILR
                     HMLDIREDMELPWSLEGTTARKSAADSARERWRPVVGGLVDEWSDAEFIDNLDYTLFP
                     NFHPWGAFNRIVYRFRPNGDDHRSSLMEVMMLAPFTGERPPPADVVRLDADQPWTDAS
                     ELGMLAKVFEQDTFNMGRVQRGLETTRKPGVTLAGYQEAKVRWLNETLDEWLER"
     gene            4154..4363
                     /locus_tag="AC16QF_13115"
     CDS             4154..4363
                     /locus_tag="AC16QF_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449546.1"
                     /translation="MKGMKHPFGRALYEQDGNGNVLVTTVDGVWGRFRTDGSWLEGEL
                     KEADPHMCGWVGGPTVAHHRIQVEE"
     gene            complement(4401..4895)
                     /locus_tag="AC16QF_13120"
     CDS             complement(4401..4895)
                     /locus_tag="AC16QF_13120"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6449547.1"
                     /translation="MSDQAFTPEEIAELRNLLEIEKIRKVKLLYSQLMDSRDIDALAD
                     IFAEDAICEFGPYGTWHGQDEIRANYHQVFKDAAPYDGIHATTNQWVELTSPTTAVSR
                     TYLIDVSHEANPRVNPVVWYGLYDEDYEKIEGAWKVKRCSLQFLWPERLVNEGWPGPF
                     PPAS"
     gene            complement(4935..>5911)
                     /locus_tag="AC16QF_13125"
     CDS             complement(4935..>5911)
                     /locus_tag="AC16QF_13125"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6449548.1"
                     /translation="CSHRGAPVVTEPEGKRPRLVCKYHGWTYSHDGELLAIRDPEDFR
                     DLDFSCRGLRPIRCERFGNLIFVNFDDQAPTLLDWLGPVAAEWEEFQFDKCRLASRHI
                     FDLNCNWKIAMEANTEVYHVRSIHPKTVAPMLDDRRNVNTLYPNGHGRMVAPVPPSAK
                     VPSIVDLPSGSSIETVGEIARTCTQSYGVFPNWVSPLNHRVLPPLLFWPNGIDKCRLE
                     TWTLAPDWGNEPKPDMWTVNDGESLSDVLLEDTEFGEWIQKSVESYGFKGVPLSYQEA
                     RIYHWNQFADRMIGIDNIPPELRVAQVIGEEWVWPNEPRLALARASAG"
BASE COUNT          921 a         1940 c         1973 g         1077 t
ORIGIN      
        1 gcggtgagca gctcgtccag ccgctttccg cgggtgtgga aatcgagccc gaagccctcg
       61 tactcgtagg gccggtagcc gaggccgacc cccagcaccg cgcgcccgcc ggatatctgg
      121 tccagcatgg cggcctgctc tgcaacggtc accggatggt gcagcggggc gaggtagacg
      181 ccggtgccca ggcgcagccg ctcggtacgc gccgccaccg ctgacagaag cgcaaacggg
      241 gcggacggat cggcgatctg tggcgtgaac gagtgatggc cgaaggtgcc catgtggtag
      301 ccgagctcgt ccgcggtgcg ggcgaagccc agtgttcgtt cgaaggggct cgcggccccg
      361 gcgtccacga acaccggcag catgagactg aacctcatgg gcgcttactc gggaatcacg
      421 ccgacacgct tggccgctga gacatccgct cgaaccacgc gtgtatgttc tgattggtct
      481 ccgcgtaggg ctggcccacg gtcttgccga aagcgaggaa gcagtacagc agcacgtcgg
      541 ccagcgtgat cctgtcgccg cagacgaact cgcggccggc catgaggccg tcgagccagg
      601 cgatcttctc cgccgcgatg cgcttcatgc cttcggaagc ttccggtagg gtggtgatgc
      661 ggtccttgaa gaaatccaat ccctcgccga agcggaagcc gtagcccagg ggctcgcaga
      721 tgttcaggtc gatgcggcgc acccacatgc gcgtttcggc gcgctcctcc ggcgtgctgc
      781 cgatcagaga cgtgcctggg ctcgtctcgt cgagatactc gcagattgcc gtgacctcgc
      841 tgacgtagtc gccgttgtcg agctcgaggc acggcgactg gccggccggg ttcttcgaca
      901 ggtagggctc ccggcggtta tcggccgcca tgaggtcgac ctcctccatg tcgagttcga
      961 tgccgcgctc ggcggcaaac gtgcgcacca cctgagggtt cgggccgatg gagttgtaga
     1021 atttcattgc gtgttacccg attgattcaa acgtcggatt ctaggggccg gcgccgctgc
     1081 gcaccaccac gggcgaccgg cgcgccgccg gcctggaggc tgatgcccgg gttgtacggg
     1141 ccgcgcgggc aggtgcaagc accgttccag gcgcgtagac tcgccgacct tccccggagc
     1201 ccggattctg ccgatagccg tgtgcccggg tccgcccccg gcggggccgt gatattgagg
     1261 catcgtggaa ggtcccgtga cgaaaggcta tcaggtcagg cttcccacca agatcgtcta
     1321 cgggcttggc agcgtttccg acggcgtcaa gaacaccacg ttcaacgttt ttctgctctt
     1381 cttctacacc caggttgcgg gcctctccgg ctcgttggcc ggtgcggcta tcctcatcgc
     1441 gctggtgttc gatgcggtca gcgacccgct catcggcaac atctcggaca actttcactc
     1501 ccgctggggc cgccgccatc cgttcatgta tgccgccgcg ctgcccatgg ccgtcttttt
     1561 cgggctgatc ttcagcccgc cacaagggct gggccaggcc ggactcttcg cctggatgct
     1621 gaccatggcc attggtggcc gggtgtccag gacggtgtac gcctttccca gcggcgcgct
     1681 ggtggcggag atgacgagcc actacgacga gcgaacgtcc ctggtcagct tccgggtgct
     1741 ctccggctgg ctgggagggc tggtcgcctc ccagatgggc tacctcgtct tctttgcccc
     1801 gtctgccgcc tacgcggacg gtcgcctgga tcctcaggct tacggcgact acgccgtctt
     1861 cggcgccatc acgatcttct gcgccatcct gatctgcgcg atcggcaccc atcgattgat
     1921 tcccgaactg aaatcgccgc ccgcgcatac accgtttacc ctgcgccggt tcggcgtcga
     1981 gctgcgcgag gtgttcagca accgcccgta tctcgtgctc gtgttcacgg tgctcgtcgt
     2041 ctccacggcc accggcttca ccgacgtcat gggtctgtac gtgaacacgt acttctggga
     2101 gttcaccacc gcgcagcttg ccatcatcgt cttcggcgcg ctggccggcg tggtagcggc
     2161 cttctcggtc acggcaacgc ttgcgcagcg aacggacaag cgcaccatgg cgctggcgat
     2221 gctgctggtg gtggtgatca ttggacctct gcccgtggtg ctgcggctgc tgggcctcat
     2281 gccggcgaac ggcgatccgc tgctgctcgg catcatgatc gcctggacgg gcgtgatcgt
     2341 gttcgtcgcg gtgggcgtga cgattctggc cggctccatg atcgccgaca ccgtggacct
     2401 caacgagctg cgcacgggca aacgccagga aggcatgttc agcgcggcca tgggcctgac
     2461 ggccaaggcg acatcgggca tcggcggctt cgttggcggt gtggtcctgg acgtggtcga
     2521 ctttccgacg ggcgtggccg cgggcgaggt ggatgcggat accctcttcg cgctgggcgt
     2581 ggccgtgggc cccggcatgt ttctgctctg gatcacggtg ttgatcatcc tgtcgcgcta
     2641 tcggctgagc cgggccgatc acgcggacat cctgggcaag ctcgaagccc gccggaacgc
     2701 cgccgagcag cccggcgggg aagccgccgg gggctagccg agcgctgctt tgcattccgc
     2761 ccagcgccgg tggcacactc ggcggtcact cagatcaaag gaaggccgcc atgtcagagg
     2821 ccgtcgacgc ccgctcgccg ggaacgacct atcaggagct gctcgacacc gactcccacg
     2881 ccgtgccgga tgtccttcgg ctggagagcc cggcgccgcg ccggctcaac gacctgtcca
     2941 tcgaccgcta cacctcccgc gactttcacg atcgggagat ggagaagctc tggtcgcggg
     3001 tctggcagtt cgcctgccgc gaggagcaca tccccaaccc gggcgactac ctggtctacg
     3061 agatcggccg gcactcttac gtggtggtgc gttctcatca gggcgcgatc aaatgcttcg
     3121 taaacgcgtg ccttcaccgg ggccgcatgc tcaagcacta cgacggcaac tgcaccgagc
     3181 tgcgctgccc gtttcacggc ttcgcctggg aaaccgacgg gcggctgaag gacataccgg
     3241 cgagctggga cttcccccag atcgtcgcct ccgagttctc tctgccggaa gcccaggtag
     3301 ccacctgggc cggtttcgtc ttcgtcaacc ccgacccgga cgcggcgccg ttccgggaat
     3361 tcctgggcgg catggccgcc cacttcgatc gctggaacct cgccgaccgc tacgtgcagg
     3421 tccatgtgat caaggagatg cgggcgaact ggaagattgc tcaggaggcc ttctgcgagg
     3481 ccttccacgt caacgccacg cacccgcaga ccatgcccta tctgggcgac accaacagcc
     3541 aggtggacgt gtgggatacg ttttcccggg tgatcacgcc gggcggcacc cccagcccgc
     3601 tgctcgactg ggagcccgac gagagcgaca tcctccggca catgctcgac atccgcgagg
     3661 acatggagct gccctggtcc ttggagggaa ccaccgcgcg caagtcggcg gccgattccg
     3721 cgcgagagcg atggcggccc gtggtcggcg gtctggtgga cgagtggtcg gacgccgagt
     3781 tcatcgacaa cctggactac accctgtttc ccaattttca cccctggggc gccttcaacc
     3841 gcatcgtcta ccgcttccgc cccaacggcg acgatcaccg gtcctcgctg atggaggtga
     3901 tgatgctggc gcccttcacc ggtgagcgcc cgcccccggc ggacgtcgtc cgcctcgatg
     3961 ccgaccagcc gtggacggac gcatcggagc tgggaatgct ggccaaggtg ttcgagcagg
     4021 acacgttcaa catggggcgc gttcagcggg gcctggagac cacgcgcaag ccgggcgtca
     4081 cgctcgccgg ctatcaggaa gccaaggtgc gctggctcaa cgagaccctg gacgaatggc
     4141 tggagcgctg aacatgaaag gcatgaaaca tccgtttggt cgggccctgt acgagcagga
     4201 cggcaacggc aacgtgctgg tgaccacggt ggacggcgtg tgggggcggt tccgtaccga
     4261 cggcagctgg ctcgagggcg agctcaaaga ggccgacccg cacatgtgcg gctgggtggg
     4321 cgggcccacg gtggcccacc atcggattca ggtggaggaa tgagcggcgc cgccccagcg
     4381 tcgcgcccgc accggtcggg ctagctggcc ggcgggaacg gccctggcca gccctcgttg
     4441 acgagccgct ccggccagag aaactgcagg ctgcagcgct tcaccttcca ggcgccttcg
     4501 atcttctcgt agtcctcgtc gtaaagaccg taccagacca ccgggttcac ccgggggttg
     4561 gcctcgtggc tgacgtcgat gaggtacgtg cggctcaccg ccgtggtggg actggtcagc
     4621 tccacccact ggttggtggt ggcgtggatg ccgtcgtagg gcgcggcgtc cttgaatacc
     4681 tggtggtagt tcgcgcggat ctcgtcctgg ccgtgccagg tgccgtaggg cccgaattcg
     4741 cagatcgcgt cctcggcaaa gatgtcggcg agggcgtcga tgtcgcggct gtccatcagc
     4801 tgcgagtaga gcagcttcac tttccgaatc ttctcgatct ccaggagatt tctcagctcc
     4861 gcgatctcct cgggggtaaa ggcctggtca gacatctctt cgtgctcctg tgttgagtgc
     4921 gcgcccgatg gccgtcagcc ggcgcttgcc cgggcgagcg cgagtcgggg ctcgttcggc
     4981 caaacccact cctcgccgat cacctgggcc acgcgcagct ccggcggaat gttgtcgatg
     5041 ccgatcatgc ggtcggcgaa ctggttccag tggtagatcc tggcctcctg gtagctcagc
     5101 gggacccctt tgaagccgta ggactccacc gacttctgga tccactcgcc gaactcggtg
     5161 tcctccagca gcacgtcgct gagactctcc ccgtcgttca ccgtccacat gtcgggtttc
     5221 ggctcgttcc cccagtccgg cgccagggtc caggtctcca gccggcactt gtcgatgccg
     5281 ttgggccaga acagcagggg agggagcacc cggtgattca ggggtgacac ccagttggga
     5341 aagacgccgt aggactgggt gcaggtgcgg gcgatctcgc ccacggtctc gatgctgctg
     5401 ccgctcggca ggtcgacgat ggacggcacc ttggcgctgg gcggcaccgg cgcaaccatc
     5461 cgtccgtgcc cgttgggata cagcgtgttc acgttgcggc gatcgtcgag catgggcgcg
     5521 acggtcttcg ggtggatgct gcgaacgtgg tagacctccg tgttcgcctc catggcgatc
     5581 ttccagttgc agttcaggtc gaagatgtgg cgggaggcga gccggcactt gtcgaactga
     5641 aactcctccc actccgcggc caccgggccc agccagtcca gcagcgtggg ggcctggtcg
     5701 tcgaagttca cgaagatcag gttgccgaag cgctcgcagc ggatgggccg caggccgcgg
     5761 caggagaaat ccagatcgcg gaaatcctcg ggatcgcgga tggccagaag ctcgccgtcg
     5821 tggctgtagg tccagccgtg atatttgcac accagacgcg ggcgtttgcc ttccggttcg
     5881 gtgacgacgg gcgcgccgcg gtggctgcac g
//
LOCUS       DBNHLM010000284        15734 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_759322, whole genome shotgun sequence.
ACCESSION   DBNHLM010000284 DBNHLM010000000
VERSION     DBNHLM010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 15734)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15734)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15734
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_759322"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..771)
                     /locus_tag="AC16QF_13130"
     CDS             complement(55..771)
                     /locus_tag="AC16QF_13130"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6449549.1"
                     /translation="MPELKVITFDLDNTLWDVDRVIINAEQRMRTWLLAHVPAFHERF
                     PPETIFELRTEIVNRQPHLKHDLSRLREEVLYQAISRCGYPEALAREYAHQAFQLFFE
                     ARHEVEFFEGALESLAILAKHYVLGALTNGNADFTKLKLDGYFSFGYSAASVGAGKPA
                     PDMFHAALEHTGAKPEQVVHVGDHPVDDIQGASQLGMHTIWVNLSGEALPPGTPKPSD
                     EVARVHDIPTAVSRIAGRGR"
     gene            complement(786..1664)
                     /gene="xerC"
                     /locus_tag="AC16QF_13135"
     CDS             complement(786..1664)
                     /gene="xerC"
                     /locus_tag="AC16QF_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HIR6449550.1"
                     /translation="MTDPAVKRFQDYLRVEKRYSPNTLAAYRRDLERLGAVARVPWTE
                     IRGHHVSTFVAKLHAEGLAPRSIQRALSSIRSFFSYLEKIKLVASNPAAGIKAPKTRM
                     KLPKTLDTDQASQLFAFEAKTPIQKRDRAMAELLYGSGIRLSELVGIDVGDMDLSAGF
                     VRVVGKGNKVRQVPMGGHCIAAIEYWLAARGHPAGDAPLFTGRGERRISPRTVQLRLK
                     RLATQQLGSNQLHPHMLRHSFASHMLESSSDLRAVQELLGHADISTTQIYTHLDFQHL
                     AKVYDAAHPRAQSQDD"
     gene            complement(1661..2335)
                     /locus_tag="AC16QF_13140"
     CDS             complement(1661..2335)
                     /locus_tag="AC16QF_13140"
                     /inference="COORDINATES: protein motif:HMM:NF016243.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HIR6449551.1"
                     /translation="MTAAQDEPTRLTDKDVISYLRLDPDFFQRHSKLLSELNLPHDTG
                     GAVSLIERQVAILRERNMAMRRRMNELVQAAKANETLFEKTRSLTLELLHVDTWHALN
                     EVLATYVLVDFHADFVCCHLRNSEIRMDHVRGHGEELPNERYSRSIDPACVTLRADEL
                     EVLFPAENHDEGGSAVLAPLVLNEAIGCLAIGSRDPAHFESDMDTLFVAYIAEVVARV
                     VQRLSP"
     gene            complement(2332..3183)
                     /gene="dapF"
                     /locus_tag="AC16QF_13145"
     CDS             complement(2332..3183)
                     /gene="dapF"
                     /locus_tag="AC16QF_13145"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016703183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR6449552.1"
                     /translation="MKLEFAKMHGLGNDFMVLDLISQDFEPNREQIHAWADRRTGIGF
                     DQMLTISAPTDPDADFRYRIYNADGTEVEQCGNGARCAARFIAERKLSPKDTLVLETL
                     AGPISTALLDAETVEVDLGVPSTRAEDVPFSPEAACGAQGDAVGTFTLRAGEGEHEVT
                     PVSVGNPHGVLFVTQITDAPVDAVGASLTRHPFFPEGANIGFCQVVDPHFIRLRVYER
                     GVGETRACGTGACAAVVAGQLLGHLEDRVKVSLPGGKVRIAWQGPETTVRMAGPATLV
                     YEGHMSL"
     gene            complement(3180..4454)
                     /gene="lysA"
                     /locus_tag="AC16QF_13150"
     CDS             complement(3180..4454)
                     /gene="lysA"
                     /locus_tag="AC16QF_13150"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR6449553.1"
                     /translation="MDAFSITDGVLHAENVPLPRIAEAVGTPTYVYSTAHLKAQYQRL
                     RRALGELDADVCYAVKANANLAILRLFAEQGAGFDIVSGGELQRVISAGGDPARVIFS
                     GVGKSVAEIDFALKLGIRCFNVESASELDCLSERARVLKRQAPMSIRVNPDVDAGTHP
                     YISTGLRSNKFGIPPGQAVELYRRAQADEYLSVHGIDCHIGSQIGTIDPLIDALRNLL
                     ELTDRLSAEGIRLEHVDVGGGLGIRYGDEEELDVEAYGAALCELLRGRELKLLLEPGR
                     YLVGNGGVLLTRTRFLKPTTAETEKNFAVVDAAMNDLIRPALYQAWHEVRPVTPRAGA
                     AELCWDVVGPVCETGDFLAERRELALQEGDLLAVMSAGAYGMVQSSNYNTRPRPAEVL
                     VAGDRFRVVRRRETTRDQLALELEAEHAETWR"
     gene            complement(4638..6017)
                     /gene="argH"
                     /locus_tag="AC16QF_13155"
     CDS             complement(4638..6017)
                     /gene="argH"
                     /locus_tag="AC16QF_13155"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR6449554.1"
                     /translation="MSRDSKPWGGRFSEATDAFVESFTASVAFDKRLYHHDIRGSIAH
                     ANMLSRIGVLTEGERNEIVSGLQAIHKEIDEGELAWRVELEDVHMNVESRLIELIGET
                     GKKLHTGRSRNDQVATDIRLYLKDAIDELVALETQLLGALVDLAEAHADTVMPGFTHL
                     QPAQPVTFGHHLLAWFEMLHRDRDRLNDCRRRVDVMPLGSAALAGTTYPIDRAGVAAE
                     LGFGAVAENSLDAVSDRDFAIEFTSVAALTMNHFSRWSEELVLWTSQQFGFVELPDRF
                     CTGSSIMPQKKNPDVPELIRGKSGRVAGNLVALLTLMKGQPLAYNKDNQEDKEPLFDT
                     VDTLRDCLTALNGLVPAITPNRERMRSAAAAGFTTATDLADYLVRRDVPFRDAHEVVG
                     RAVAHAIGQGKGLDELTIEELRAFSDSIGEDVFSVLTLEGSVSARNHPGGTAPDSVRE
                     AAARARDRL"
     gene            6043..7083
                     /locus_tag="AC16QF_13160"
     CDS             6043..7083
                     /locus_tag="AC16QF_13160"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6449555.1"
                     /translation="MADYSNHRERFVVPELCNAAAISLLVLFGELLVLVLLFAGGPIT
                     WLRLALMSLFVQWVVIMSAGALCWTRAWLSRFAVVPGAVLAFSMVMAITLFVGISADR
                     IMAPPGVEAINWMGILGQLLISGIITSLALRFLYVQQQLRAQEQSELQSRIQALQSRI
                     RPHFLFNSMNIIASLIATDPETAESVVEDLSELFRASLNDAGNQVPLQDELELCERYV
                     RIESLRLGDRLRVEWNVDDSGQNLKIPLLTLQPLLENAIYHGVQPLPEGGTITVHLWL
                     EGNDVNVRITNPIPEGEASNSSAGNRMAVNNIRSRLMVLYGTRAELRAAPEEGLFVTS
                     LKYPVSNMPLQT"
     gene            7169..7897
                     /locus_tag="AC16QF_13165"
     CDS             7169..7897
                     /locus_tag="AC16QF_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003157680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR6449556.1"
                     /translation="MRVLIVDDEQLARDRLSRMVAELDDYEVAGEAGTGQDAVRLVAD
                     LQPEIILLDIRMPGMDGLEAARHLESVDEPPAVIFCTAYEEHAIEAFNLQAVGYLLKP
                     VRRENLRQALGNAQRVNKAQLAALSDDGSRRRSHISARTRKGIELVPLEAVRYFQADQ
                     KYVTVRHGEGELIIDETLRELEREFGELFIRVHRNALVAARHIIALDRLGDGHYQIRL
                     NDVDEHIDISRRHVASVRKFVRNL"
     gene            7894..8811
                     /gene="hemC"
                     /locus_tag="AC16QF_13170"
     CDS             7894..8811
                     /gene="hemC"
                     /locus_tag="AC16QF_13170"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIR6449557.1"
                     /translation="MSALRLVIATRQSQLALWQANFVSARLREAHPGLTVELLGITTR
                     GDRWLSSPLAEVGGKGLFIKELEEALASGTADVAVHSVKDVPAELPEGFAMPVIAYRE
                     DPRDVLVSRDGVTLAGLHAGARVGSSSLRRQAQLLARRPDLLIEPIRGNVDTRLRKLD
                     DGDYDALVLAAAGLARLGLAGRASEYLATDDCLPAAGQGALGIECRAVDGRVRALLEP
                     LEDAQVAACVCAERTVSAVLGADCTAPLAAHATPLGQGVMLQSRLATTDGRKILEARG
                     EGADPVAVGKSVAESLLAQGAAEVLGGGD"
     gene            8813..9514
                     /locus_tag="AC16QF_13175"
     CDS             8813..9514
                     /locus_tag="AC16QF_13175"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIR6449558.1"
                     /translation="MRVWVTRTQPGADRQARRLRAAGHEPVVGPALRIVEVPGGAPRG
                     PFTDVVYLSEHAVRFGIARLTNQAWFSGARAHAVGAATAEHLAVAGIRAGFPEQASSE
                     GLLSLPEFADARGLTVLIVCGEGGRDLLETTLTARGARVEKLVVYRRRAVDEAPPDVG
                     SVDAIAVSSGDGFAAVARLWFAAGGRGDVPVLAPSPRVAGLGSALGFSNVYTCAGAGA
                     DALLKALESVSSTGR"
     gene            9514..10617
                     /locus_tag="AC16QF_13180"
     CDS             9514..10617
                     /locus_tag="AC16QF_13180"
                     /inference="COORDINATES: protein motif:HMM:NF016275.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR6449559.1"
                     /translation="MDDDTSKGPAGDDAIGEPAPEATESASRTSGEAADQAAKAASAK
                     PRGRLLGGLGLLLALGALCGVGYLYYLLVFLGPSDELDARLGRLEASLPEIRRDLAAA
                     RDAQAEEIESAVAAQQASLDETRRALLGALNEVTAAAAPAPREWKIAEVEYLLRIANH
                     RVLMEGDVEAALQLMGAADVILEELDDFAFFQVRARLADEMLALKGVRGGDLQGVYLR
                     LEAIKTSIGELPVRLPDYFRPESSADASVEAEGFWSALAAELSGYFQLRRFDGATKPL
                     MAPEENAYLELNLRLAMERAQLAVLRRNQLVFERSLSSAREWILELLDPDDPSVEQVA
                     EELDALSTLKLERDLPDVSGSLTALLELRRGNP"
     gene            10614..11828
                     /locus_tag="AC16QF_13185"
     CDS             10614..11828
                     /locus_tag="AC16QF_13185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HIR6449560.1"
                     /translation="MKTGIVLIAIAAVLGGLVGTLVVRDPGYVLLAYDDMAVETSLWF
                     ALVALVVIYFVVRLLVFAFSRLAATGGSFGAWNRQRQSKKAREQTIRGLLLMAEGEWA
                     EARRLLVLAAPRVDSPLINYLSAARGAHATGDVEGRDELLRKAHQSTPGARFAVGLTQ
                     AQLQASAEQWEQCLASLLQLQSESPRHQLVLSMLAECYEALQDWRALIELLPGIRRAK
                     LRDKAQLDELQKRAWSRRLAATTEDLTGLWAQMPKELRRNPELVAVLCDRLLEAGLAN
                     DAEAAVRGALEHEWRSELVQRYGVIVSDDVGRQLIVAEGWLKSRPNDSALLLALGRIC
                     LMNEQWAKAREYLEASLRLRHTPEVYGELGRLCVAMGDVDRGSQYLKQSVIGLPDLPL
                     PEGEPGAAAGTG"
     gene            complement(11825..12523)
                     /locus_tag="AC16QF_13190"
     CDS             complement(11825..12523)
                     /locus_tag="AC16QF_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase domain-containing protein"
                     /protein_id="HIR6449561.1"
                     /translation="MQARIVRRTGAPVQEIVLQVEPDFAFAAGQYLEILLPNGARIPL
                     SIASGPYRLPDVHLHYRSTPGIAEAVLFDELLQTSQTLDVAGPFGDVRLELPLAGPIL
                     IAAGGTGASQALGMLDALAQAAPRGEVPFEVSFLWCADREADLYRRDWLASLNADWLS
                     TTLIADSRRDRDNRALAWLRAQSARFRDRRIFLCGGPQFVYAAADALQAGGVPRGQLA
                     ADAFGYAPRPDNSG"
     gene            complement(12535..13797)
                     /gene="rho"
                     /locus_tag="AC16QF_13195"
     CDS             complement(12535..13797)
                     /gene="rho"
                     /locus_tag="AC16QF_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR6449562.1"
                     /translation="MNLTDLKRKPVGDLIDMAREMGLENLARSRKQEVIAAIVRKQAK
                     EGEDIYGEGTLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDSIAGKIR
                     PPKDGERYFALLKVDEINFSEPNSKKHKILFENLTPLFPDDRLVLERGNGSTEDLTAR
                     IIDLIAPIGKGQRALIVSPPKAGKTLVLQNIAQSIAANNPEVVLIVLLIDERPEEVTE
                     MQRMVQGEVVASTFDEPPSRHVQVAEMVIEKAKRLVEHQKDVVILLDSITRLARAYNT
                     IVPSSGKVLTGGVDAHALERPKRFFGAARNIEEGGSLSIIATALVDTGSKMDEVIYEE
                     FKGTGNQELHLERKIAEKRVYPAINIRRSATRREELLMGEEELQRVWILRKLLHDMDD
                     IAAIEFMIDKLKDTKTNEEFFALMRGGR"
     gene            complement(14041..14367)
                     /gene="trxA"
                     /locus_tag="AC16QF_13200"
     CDS             complement(14041..14367)
                     /gene="trxA"
                     /locus_tag="AC16QF_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HIR6449563.1"
                     /translation="MSDNIVHTSDSDFDNDVLSSDKPVLVDYWAEWCGPCKMIAPILD
                     EIAEEYSDRIKVCKLDIDANQATPPKYGIRGIPTLMLFKNGEVEATKVGALSKSQLAA
                     FLDSNI"
     gene            complement(14481..15473)
                     /gene="hemB"
                     /locus_tag="AC16QF_13205"
     CDS             complement(14481..15473)
                     /gene="hemB"
                     /locus_tag="AC16QF_13205"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011336494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR6449564.1"
                     /translation="MPRSFPATRLRRNRQTDFIRRLVRETELTVNDLIYPVFVVEGAD
                     QQIAVDSMPGINRLSIDNLLRHAEEAAKFGIPAIALFPYTQPDLRTPDGCEAWNPDGL
                     IPRAVAALKEALPDLGVMTDAALDPYTTHGQDGIIDESGYVLNDVTVEALCRQAAVCA
                     NAGADVIAPSDMMDGRVGAIRQMLESEGHDNTLIMAYSAKYASAYYGPFRDAVGSSAT
                     LGTGSKHTYQMDPANSDEALQEIALDLEEGADMVMVKPGMPYLDIIRRVKDRFGVPTF
                     AYQVSGEYAMHMAAVGNGWLDQSVVAESLLCLKRAGADGILTYFAFTVAAALAD"
BASE COUNT         2518 a         5022 c         5320 g         2874 t
ORIGIN      
        1 gtcgaggcgg tcaaggaggc gattcaggcc agcgcccgct gagggcgatt cggatcagcg
       61 gccgcgcccg gcgatgcggg atacggccgt gggaatgtcg tgcacccttg cgacctcgtc
      121 cgagggcttc ggggtcccgg gcggcagcgc ctcgccgctc aggttgaccc agatggtgtg
      181 catgccgagc tgggatgcgc cctggatatc gtcgaccggg tggtcgccca cgtggaccac
      241 ctgctcgggc ttcgctcccg tgtgctcgag ggccgcatga aacatgtccg gcgcgggctt
      301 gccggcaccg acgctggccg cggagtaacc gaaagaaaaa tagccgtcca gcttgagctt
      361 ggtgaagtcg gcgttgccgt tggtcagagc gccgaggacg tagtgcttgg ccaggatggc
      421 caggctttcc agcgcaccct cgaagaactc cacctcgtgg cgcgcctcga agaacagctg
      481 aaaggcctga tgggcgtatt ctcgggccag cgcttccggg tagccgcagc ggctgatggc
      541 ctggtagaga acctcttcgc gcagtcggga caggtcgtgt ttcagatggg gctgcctgtt
      601 cactatttcc gtgcgcagct cgaagatcgt ctccggcgga aaacgttcgt ggaaggctgg
      661 aacgtgtgcg agcagccacg tccgcatgcg ctgctcggcg ttgatgatga cccgatccac
      721 atcccacagg gtgttgtcga gatcgaaggt gatgactttc agctcgggca cgatcagggg
      781 cctaatcagt cgtcctggga ttgcgcgcgg ggatgggcgg cgtcgtacac ctttgccaga
      841 tgctggaagt ccagatgtgt gtagatctgg gttgttgaga tgtcggcgtg gccgagaagt
      901 tcctgaaccg cccgcaggtc gctggacgac tcgagcatgt gactggcaaa gctgtgcctg
      961 agcatgtgcg ggtgcagctg gttgctgccg agctgctggg tggccaggcg ctttaggcgc
     1021 agctggacgg ttcgggggct gatacgccgc tcgccccgac cggtgaacag cggcgcgtcg
     1081 ccggcgggat gtccccgggc tgccagccag tattcgatgg ccgcgatgca gtgcccgccc
     1141 atggggacct gcctgacttt gttgcctttg ccgacgaccc tcacgaaccc ggcgctcagg
     1201 tccatgtcgc cgacgtcgat gccgaccagc tcggacaggc gaattccgct gccgtagagc
     1261 agctcggcca tcgcgcggtc acgtttctga atcggcgttt tggcctcgaa ggcgaacagc
     1321 tgtgaggcct gatccgtatc gagcgtcttg ggcagcttca tccgggtctt tggcgctttg
     1381 atgccggctg cggggttgct ggcgaccagc ttgatcttct ccagatagct gaagaaggaa
     1441 cggatgctcg acagggcgcg ctggatgctt cgcggagcca gcccttcggc gtgcagcttg
     1501 gcaacgaacg tgctcacgtg gtgtccgcgg atctcggtcc agggcaccct ggccaccgcg
     1561 cccagccgtt ccagatcccg ccggtaggcg gccagcgtgt tgggtgagta gcgcttctcg
     1621 acccgcaggt agtcctggaa ccgcttgacg gcgggatcgg tcacggcgac aggcgctgga
     1681 ccacccgcgc gaccacctcc gcgatgtagg cgacgaacag cgtatccatg tctgattcga
     1741 agtgagcagg atcgcggctg ccgatggcga ggcagccgat cgcctcgttg agcaccagcg
     1801 gtgccagcac cgcgctgccg ccctcatcgt gattctccgc ggggaaaagc acctcgagct
     1861 cgtcggcgcg cagggtgacg cacgcgggat cgatgcttct ggagtagcgc tcgttgggaa
     1921 gttcttcgcc gtggccccgc acgtggtcca tgcggatctc cgaattcctc agatggcagc
     1981 atacgaagtc cgcatggaaa tccaccagca cgtaggtcgc gagcacctcg ttcagagcgt
     2041 gccacgtgtc cacgtggagc agctccagcg tcagggaacg cgtcttctcg aacaacgtct
     2101 cgttggcctt ggccgcctga acgagctcgt tcatccggcg ccgcatcgcc atgttgcgtt
     2161 cgcgaaggat ggctacctga cgctcgatca gcgagaccgc gccgcccgtg tcgtggggca
     2221 ggttgagctc ggaaagcagc ttcgagtgcc gctggaagaa gtcggggtcg agtcgcaggt
     2281 aagaaatgac gtctttgtcg gtaagcctcg tcggctcgtc ctgggccgcg gtcatagact
     2341 catgtggcct tcgtaaacca gtgtggcggg tccggccatt ctaaccgtgg tctccgggcc
     2401 ctgccaggcg atgcggacct tgcctcccgg caacgatacc ttcacccggt cttccaggtg
     2461 gcccagcagc tggccggcaa cgacggcggc gcatgcgccc gttccgcaag cccgcgtttc
     2521 gcccacccct cgctcgtaga cgcgcagccg gatgaaatgc ggatccacca cctggcagaa
     2581 cccgatgttc gcgccctcgg gaaagaacgg gtggcgggtc aggctggccc ccaccgcgtc
     2641 gacgggcgca tcggtgatct gcgttacgaa gagcaccccg tgaggattcc cgacggagac
     2701 cggggtcacc tcatgctcgc cctcccccgc gcgcaacgtg aaggtgccga ccgcatcgcc
     2761 ctgcgcgccg cacgccgcct ccgggctgaa cggcacgtct tcggcccggg tgcttggaac
     2821 cccaagatcg acctccacgg tctcggcgtc cagcagggcc gtggatatgg gccccgccaa
     2881 cgtctcgagc accagggtgt ctttcgggct cagctttcgc tcggcgatga atctggccgc
     2941 gcatcgggcg ccgttgccgc actgctccac ctcggtgccg tccgcgttgt agatgcggta
     3001 gcggaagtcc gcatccgggt cggtgggcgc cgagatggtg agcatctgat cgaagccgat
     3061 cccggtgcgg cgatcggccc aggcgtgaat ctgttcccgg ttcggctcga aatcctggct
     3121 gatcaggtcc agcaccatga agtcgttacc gagcccgtgc atcttcgcga actcgagctt
     3181 catcgccacg tctccgcgtg ctccgcctcg agctccaggg cgagctgatc gcgagtggtt
     3241 tcgcggcgcc gcaccacccg gaagcggtct ccggcgacca gaacctccgc gggtctggga
     3301 cgcgtgttgt agttggagct ctgcaccatc ccgtaggccc cggcggacat gacggccaga
     3361 aggtcgcctt cctgaagggc cagttctctg cgttcggcca ggaagtcgcc ggtctcgcag
     3421 acggggccca ccacgtccca gcacagctcg gccgcccccg ctcgcggcgt gacgggacgg
     3481 acctcgtgcc acgcctggta cagcgccggg cggatcagat cgttcatggc agcgtcgacc
     3541 accgcgaagt tcttctcggt ctcggccgtc gttggtttca ggaatcgcgt tcgggtcagc
     3601 agcacgccgc cgttccccac cagatacctg ccgggttcca gcaggagctt gagttcccgg
     3661 ccgcgcagca gctcgcaaag ggccgcaccg tatgcctcga cgtcgagctc ctcctcgtcc
     3721 ccgtagcgga tgcccagccc gccgcccacg tccacgtgtt ccaggcggat tccctccgcg
     3781 gacagccggt cggtgagttc cagcaggttg cgaagggcgt cgatcagcgg gtcgatggtg
     3841 ccgatctggg agccgatgtg gcagtcgatc ccgtgcacgg ataggtactc gtctgcctgc
     3901 gcccgcctgt acagctcgac cgcctggccc ggcggtatac cgaacttgtt gctgcgtaac
     3961 cccgtggaga tgtagggatg cgtcccggca tccacgtccg ggttgacccg gatggacatg
     4021 ggcgcctggc gcttgagcac ccgtgcccgc tcgctcaggc agtcgagctc gctggcactc
     4081 tccacgttga agcagcggat gccgagcttg agcgcgaagt cgatctcggc caccgacttg
     4141 cctaccccgg agaaaatcac cctggcaggg tcgcctcctg cgctgatgac ccgctgcagc
     4201 tcgccgccgg agacgatgtc gaaaccggcg ccctgctcag cgaagagcct cagaatggcc
     4261 agattggcgt tggccttgac cgcgtagcag acgtccgcgt cgagctcgcc gagcgcgcgg
     4321 cgcagccgct ggtactgcgc cttcaggtgg gcggtcgagt agacgtacgt cggcgtgccg
     4381 accgcctcgg caatccgcgg cagcggcacg ttctccgcgt ggagcacccc gtctgtgatg
     4441 gagaaggcgt ccacgtcagg gccggtggct ggcggcgggc gcttctgact gagcgtcggg
     4501 cagctccagg ggacccttct gaccgcacat gaccgcctga gaggcaagaa tgattaacag
     4561 gatccagcgc accagtggct cgcagctagg ccgaacgagt cagtatacac cgcctcatgg
     4621 cggcgcagcg gccgcggtca aagccggtcg cgggcgcggg ctgccgcctc gcgcacgctg
     4681 tcgggggcgg tgccgcccgg gtgattgcgc gcactgacgg acccctcgag cgtgagcacg
     4741 gaaaacacgt cctcgcctat gctgtcgctg aacgcgcgca gctcctcgat cgtcagctcg
     4801 tcgagaccct tgccctgccc gatcgcgtgc gccacggcgc ggcccaccac ctcgtgggcg
     4861 tcccggaagg gcacatcgcg ccgcaccagg taatcggcca ggtccgtcgc ggtggtaaag
     4921 cccgcggcgg cggccgaacg catccgctcc cgattggggg tgatggccgg gacgaggccg
     4981 ttcagagccg tcaggcaatc gcgcagggtg tccaccgtgt cgaacagcgg ctccttgtcc
     5041 tcctggttgt ccttgttgta ggccagcggc tggcctttca tcagcgtcag cagagccacc
     5101 aggttgccgg ccaccctgcc cgacttgccc cggatcagct cggggacgtc ggggttcttc
     5161 ttctgcggca tgatgctcga gcccgtgcag aaacgatcgg gcagctcgac gaatccgaac
     5221 tgctgggacg tccacagcac cagctcctcg ctccagcgag aaaagtggtt catcgtcaac
     5281 gcggcaaccg acgtgaactc gatggcgaag tcccggtcgc tcaccgcgtc cagcgagttc
     5341 tcggccacgg cgccgaatcc gagctcggca gcgacaccgg cccgatcgat gggataggtg
     5401 gtgccggcaa gcgccgcgct gcccaggggc atgacgtcca cgcgccggcg gcagtcattg
     5461 aggcggtcgc ggtcgcggtg aagcatctcg aaccacgcca gcagatggtg gccgaacgtt
     5521 acgggctgcg ccggctgcag atgcgtgaaa ccgggcatga cggtgtccgc gtgggcttcg
     5581 gccaggtcca ccagcgcgcc gagcagctgg gtttccaaag cgaccagctc gtcgatggcg
     5641 tccttcaggt agagccggat atcggtggcg acctggtcgt tgcgggaccg gcccgtgtgc
     5701 agctttttcc cggtttctcc aatgagctcg ataaggcgcg actccacgtt catgtggacg
     5761 tcctcgagct caacccgcca ggcgagctcg ccttcgtcga tctctttgtg aatcgcctgc
     5821 aatcccgata cgatctcgtt gcgctcacct tcggtgagca cgccgatccg gctcagcatg
     5881 ttggcgtggg cgatggagcc cctgatatcg tgatggtaaa gacgcttatc gaatgccacc
     5941 gaggccgtga acgattcgac gaacgcatcg gtggcttccg aaaagcggcc gccccagggt
     6001 tttgaatctc ggctcatggt gacaagaggg ttcctttagc ggatggccga ttatagcaac
     6061 catcgcgagc ggttcgtggt ccccgagctt tgcaacgcgg cggccatttc cctgctcgtg
     6121 ctcttcggtg agcttctggt tctggtgctg ctgttcgccg gcggccccat cacgtggctc
     6181 cggctcgcgc tcatgtccct gttcgtgcag tgggttgtca tcatgagcgc gggagccctg
     6241 tgctggacgc gagcctggct ctcccggttc gcggtggtgc ctggcgccgt gctcgctttc
     6301 tcgatggtca tggcgatcac cctgttcgtc gggatcagcg ccgatcggat catggctccg
     6361 cccggcgtgg aagcgatcaa ctggatgggc attctcggcc agctcctcat ttccgggatc
     6421 atcacttccc tggcgctgcg cttcctgtac gtccagcagc agctgcgcgc tcaggagcag
     6481 tcggagctcc agtcgcgcat ccaggcgttg cagtcgcgca ttcgcccgca cttcctgttc
     6541 aacagcatga acatcatcgc aagcctcatc gccaccgacc ccgagaccgc ggagtctgtc
     6601 gtggaggacc tctccgagct ctttcgtgcc agcctcaacg acgcgggaaa tcaggtgccg
     6661 ctgcaggacg agctggagct ttgcgagcgt tacgtgcgca tcgagtccct gcggctcggt
     6721 gatcggctgc gcgtcgaatg gaacgtggac gactcgggcc agaatctcaa gattcctctg
     6781 ctgactctgc agccacttct ggaaaatgcc atctaccacg gggtccagcc gctgccggaa
     6841 ggcggcacga tcaccgttca tctgtggctg gaggggaatg acgtcaacgt tcgcatcacc
     6901 aacccgatac cggagggcga ggcgagcaac agcagcgcgg gaaaccgaat ggcagtcaac
     6961 aacattcgca gcaggctgat ggtgttgtac ggtacccgtg cggaattgag ggcggcgccc
     7021 gaggaagggc tgttcgtcac gtcgctgaag tacccggtgt cgaacatgcc gttgcagaca
     7081 tgagcgcggc ggcgagcgcg gcggcaagcg cggcggcgag cgcggcggcg agcctggaga
     7141 ccagcacgct cagggagggg atatgagcat cagggttctc atcgtggacg acgaacagct
     7201 ggcccgagat cgcctgtcgc gaatggtggc cgagctggac gattacgagg tagccggtga
     7261 ggctggcacc gggcaggacg ccgtgcgcct ggtggctgac ctgcagccgg agatcatcct
     7321 gctcgatatc cgaatgccgg gcatggacgg gctggaagca gcgcgtcatc tggagtcggt
     7381 cgacgagccc ccggccgtca tcttctgcac ggcctacgag gagcacgcca tcgaggcgtt
     7441 caacctccag gccgtcgggt accttctgaa acccgtgcgc cgggagaacc tgcgccaagc
     7501 gctcggcaac gctcagcggg tcaacaaagc tcagctggca gcgctctcgg atgacggcag
     7561 ccgacgccgc tcccatatct ccgcgcgcac gcgcaagggc atcgagctcg tacccctcga
     7621 agccgtgcgc tactttcagg ccgatcagaa gtacgtcacc gtgcgccacg gcgagggcga
     7681 gctgatcatc gacgaaaccc ttcgcgaact agagcgcgag ttcggcgagc tgttcatccg
     7741 cgtccatcgc aacgccctgg tggccgcgcg ccacatcatc gcgctcgatc gtctgggcga
     7801 cggccactac cagatccgcc tcaacgacgt cgacgagcac atcgacatca gccggcgcca
     7861 cgtggcctcc gtgcgcaagt ttgtcaggaa cctgtgagcg cattacggct cgtcatagcc
     7921 acgcggcaga gtcagcttgc cttgtggcag gccaacttcg tcagcgcgcg tcttcgcgag
     7981 gcccatccgg gcctcaccgt ggaactcctc ggcatcacca cccgtgggga ccgatggctt
     8041 tcttcgcccc tcgccgaggt cggcggcaag ggcctgttca tcaaggagct cgaagaagcg
     8101 cttgcctcgg gcacggccga cgtcgccgtg cactcggtaa aggacgtgcc ggcggagctt
     8161 cccgaaggat tcgccatgcc ggtcatcgcc tatcgtgagg atccccgtga cgtgctggtt
     8221 agccgggatg gggtgactct ggccgggttg cacgccggtg cccgggtcgg ctcatcgagc
     8281 ctgcgcaggc aggctcagct cctggcccgg cgccccgatc tgctgatcga gcccatccgg
     8341 ggcaacgtgg acacccggct gcgcaagctg gacgacggcg attacgacgc cctggtgctt
     8401 gccgcagcgg gtctcgcccg gctgggactg gcggggcgcg ccagcgagta cctggcaacc
     8461 gacgactgcc tgcccgccgc cggccaggga gcgctgggca tcgagtgccg ggcggtcgac
     8521 gggcgcgtgc gggcactgct cgaacccctc gaagatgctc aggttgctgc ctgcgtttgc
     8581 gccgagcgca cggtgagcgc cgtgctggga gcggattgca cggccccgct ggccgcacac
     8641 gccacgccgc tcggccaagg cgtgatgctg caatcccgtc tggcgaccac cgacggccgg
     8701 aagattctgg aagcccgtgg cgagggggcc gatccggtag ccgtgggcaa atcggtcgcc
     8761 gagtcgcttt tggcgcaggg cgcggccgag gtgctcggcg gcggagattg agttgcgcgt
     8821 ctgggtgacc cgaacccaac ccggcgccga ccgccaggcc cgaaggctga gagcggccgg
     8881 ccacgagccg gtcgtgggcc cggcgctgag aatcgtggag gtgcccggcg gcgcgcctcg
     8941 cggacctttt accgacgtgg tctatctttc cgagcatgcc gttcggtttg gcattgcgcg
     9001 cctgaccaac caggcgtggt tctccggtgc gcgcgcccat gccgtgggag cagcgaccgc
     9061 cgagcacctg gccgttgccg ggattcgtgc gggcttccct gagcaggcga gctccgaggg
     9121 gctgctctcg ctgccggaat ttgccgatgc ccggggcctg acggtgctca tcgtctgcgg
     9181 agaaggaggg cgggatctgc tcgagacgac gttgacggca aggggcgcca gggtggaaaa
     9241 gctcgtcgtc taccggcgcc gcgccgtgga cgaggcccct ccggatgtcg gctcggtgga
     9301 cgccatcgcc gtctccagcg gcgatggatt cgcggcggtg gcgcggttat ggtttgccgc
     9361 aggcggtcgc ggggacgtgc cggtgttggc gccgtcccca cgggtggccg ggctgggttc
     9421 cgcattgggg ttcagtaacg tgtacacatg tgcgggagcc ggcgccgatg ccctgctaaa
     9481 ggcgctcgag tccgtgagtt cgacgggtag gtgatggacg acgataccag caaaggtccg
     9541 gccggcgacg acgccatcgg tgagcccgcc ccggaggcga cagaatccgc ttccaggacc
     9601 tcaggggagg cagctgacca ggccgcgaag gcagcgtcgg caaaaccgcg cggccgcctt
     9661 ctcggcgggc ttggcctcct gctcgccctg ggcgcgcttt gcggggtcgg ctatctctat
     9721 tacctcctcg tcttcctggg accgtcggat gagctcgatg cccggctcgg gcgcctggag
     9781 gcgtctcttc cagagatccg ccgagatctc gcggcggcgc gcgacgcgca ggccgaggag
     9841 atcgagagcg ccgtcgccgc tcagcaggcg tcactggacg agacccgtag agcgctcctc
     9901 ggggcgctca acgaggtcac tgccgccgcg gcccccgccc cccgggaatg gaagatcgcc
     9961 gaggtggagt acctgctcag gattgccaac catcgggtgc tcatggaggg tgacgtggag
    10021 gccgccctgc agctcatggg ggctgctgat gtcattctcg aggagctcga cgattttgcc
    10081 ttctttcagg ttcgcgcccg gctggccgac gagatgctgg cgctcaaggg cgtccgcggc
    10141 ggcgacctgc agggcgttta ccttcgcctg gaagccatta agaccagcat cggcgagttg
    10201 cccgtccggc tgcccgacta cttccgaccg gagagctctg cagacgcgtc tgtggaggct
    10261 gaaggcttct ggagcgcgct ggccgcggag ctttccggct attttcagct gcgccgattc
    10321 gacggcgcga ccaagccgct catggcgccg gaagagaacg cttacttaga gctcaacctg
    10381 cggctggcca tggagcgtgc ccaactcgcc gttctgcgcc gcaatcagct cgtcttcgag
    10441 cggagcctct ccagcgcccg cgaatggatt ctcgagttac tcgaccctga cgatccgagc
    10501 gtcgagcagg ttgccgagga gctggacgcg ctgagcacgc tcaagctcga gcgggatctg
    10561 cccgacgtgt cgggctccct caccgcgctg ctcgagctcc gccggggcaa cccgtgaaga
    10621 cgggcattgt gctgatcgcc atcgccgccg tgctgggcgg gctggtcggc acgctggtgg
    10681 tgcgtgaccc ggggtacgtg ctgctggctt atgacgacat ggccgtcgag accagtctct
    10741 ggttcgcgct tgttgctctg gtcgtcatct atttcgtcgt gcgtctgctt gttttcgcat
    10801 tctcacggct ggccgccacg ggcggcagct tcggcgcatg gaaccgccag cggcagtcga
    10861 agaaggcgcg tgaacagacg attcgcgggc tgctgctcat ggccgaggga gagtgggccg
    10921 aagcgcggcg gctgctcgtt ctggccgccc cacgggtgga ttcgcccctg atcaactact
    10981 tgagcgccgc tcggggcgcc cacgccacgg gcgacgtcga aggccgcgac gagctgcttc
    11041 gcaaggccca ccagtcgaca ccgggggcgc gattcgccgt ggggctcacc caggcgcagc
    11101 tgcaggcttc ggctgagcag tgggagcaat gcctggcttc gcttctccag ctgcagtccg
    11161 agtcgccgcg tcatcagctg gtgctgtcta tgcttgcgga gtgttacgag gcgctgcagg
    11221 actggcgggc tctcatcgag cttctgccgg gcatccggcg cgcaaagctg cgtgacaagg
    11281 cccagctcga cgagctgcaa aagcgcgcct ggagccggcg gctcgcggcg accacggaag
    11341 acctcacggg tctttgggcg cagatgccca aggagctgcg ccgtaacccg gagctcgttg
    11401 ccgtgctctg cgaccggctg cttgaagcag ggcttgcgaa cgatgccgag gccgcggttc
    11461 ggggcgccct ggaacacgag tggcgcagcg agctggtcca gcggtacggt gtgatcgtct
    11521 cggatgatgt gggcaggcag ctcatcgtgg ccgaggggtg gttgaagtcc cggcccaacg
    11581 attcggcgct gctgctggcc ctgggtcgaa tctgcctgat gaacgagcag tgggccaagg
    11641 cccgcgagta cctcgaagcg agcctgcgcc tgcggcacac gcctgaggtc tacggtgagc
    11701 tgggccgcct gtgcgtcgcc atgggcgacg tggaccgcgg cagccagtat ctcaagcagt
    11761 cggtgatcgg gctgccggat ctgccgctgc cggaaggcga gccaggcgcg gcggcgggga
    11821 ccggctagcc cgagttgtcc ggacggggcg cgtagccgaa cgcatcggcg gccaattgcc
    11881 cgcggggcac accgccggct tggagcgcgt cggccgctgc gtagacgaac tgcgggcccc
    11941 cgcacaggaa gatccgccga tcccggaacc gcgccgattg cgcccgcagc caggcaagcg
    12001 cccgattgtc ccgatcccgt ctcgaatccg cgatcagcgt ggtagacagc cagtccgcgt
    12061 tcagcgaggc aagccagtcc ctgcgataca gatcagcctc ccggtccgcg caccagagaa
    12121 acgagacctc gaacgggacc tcaccgcgcg gcgctgcctg cgcgagcgcg tcgagcatgc
    12181 cgagcgcctg gctcgcgccc gttccccctg ccgcgatgag aatcgggccg gccagaggca
    12241 actccagccg gacgtcgcca aaagggccgg cgacatcgag ggtttggctc gtttggagca
    12301 gctcatcgaa aaggacggcc tcggcgatgc cgggggtgga tcggtaatgc aggtgcacgt
    12361 cggggaggcg gtagggacct gaggcgatgg aaagtggaat gcgcgcgccg ttcggaagaa
    12421 gtatttccag gtactggccc gcagcgaaag cgaagtctgg ctctacttgc aggacgatct
    12481 cctgcacggg tgcgcccgtg cggcggacga tcctggcctg cacggttggc tcggtcagcg
    12541 accgccgcgc atcagcgcga agaactcctc gttggtcttc gtgtccttca gcttgtcgat
    12601 catgaactcg atggcggcga tgtcgtccat gtcgtggagc agcttgcgca ggatccagac
    12661 ccgctgaagc tcttcctcgc ccatcagcag ctcctcgcgg cgggtggccg agcggcggat
    12721 gttgatggcc gggtagacgc gcttttctgc gatcttgcgt tccaggtgaa gctcctggtt
    12781 gcccgttccc ttgaactcct cgtagatgac ctcgtccatc ttggagccgg tatccaccag
    12841 ggcggtggcg atgatggaca ggctgccgcc ctcttcgatg tttcgggccg caccgaagaa
    12901 tctcttgggg cgctccagcg cgtgagcgtc gacgccgcca gtcagaacct ttccggacga
    12961 cggcactatg gtgttgtagg cccttgcgag acgggtgatc gaatccagca gaatcacgac
    13021 gtccttctgg tgctccacca gccgctttgc cttctcgatg accatctccg ccacctggac
    13081 gtgacgggag gggggctcgt cgaacgtgct cgccaccacc tcgccctgaa ccatgcgctg
    13141 catttccgtg acttcctcgg gccgctcgtc gatgagcagc acgatcagca cgacctcggg
    13201 gttgttggcc gcgatggact gagcgatgtt ctgcagaacc agcgtctttc ccgctttcgg
    13261 gggagagacg atgagcgctc gctggccttt gccgatgggc gcaatgaggt cgatgatgcg
    13321 cgcggtcaga tcctcggtgc tgccgttgcc gcgctcgagc accagccgat cgtcggggaa
    13381 gagcggggtg aggttttcga aaagaatctt gtgtttcttg gagttaggct cgctgaaatt
    13441 gatctcgtcg accttcagca gagcgaagta gcgctcgccg tccttgggcg gccggatctt
    13501 gccggcaatg ctgtcgcccg ttcgcaggtt gaatcggcgt atctggctag gcgagacgta
    13561 gatgtcgtcg ggccccgcga gataggaact gtctgcggat cgcagaaaac cgaagccgtc
    13621 ctggagaatt tccagggtcc cctccccgta gatgtcctcg ccctctttgg cttgcttgcg
    13681 gacgatggcg gcgatgacct cctgcttgcg cgaacgcgca aggttctcca gccccatctc
    13741 gcgggccatg tcgatcaggt cgccgacggg ttttctcttc aggtctgtaa ggttcatggg
    13801 cataagcgca aaccggcagg ttgtaaaaac gacgaacgga ctgttcttag atttttatgg
    13861 ctgttctttg ggtgctgcga ccggcgaagc gcggtggcgg cgggtgggcg ctggagttcg
    13921 actccagcga ttgcagccaa gtttagtgta ttgaaagacc ccgtcaagca cccctttggt
    13981 cgcacaatcc gggtttgtga acccgtttac gcgccgcctg acggggccga taggtggata
    14041 tcagatgttg ctgtccagga aggcggccag ctgagacttc gacaacgcgc ccaccttggt
    14101 cgcttcgacc tcgccgttct tgaacagcat gagggtaggt atgccacgga tgccgtactt
    14161 gggcggcgtg gcctggttgg cgtcgatgtc tagcttgcag accttgatgc ggtcggagta
    14221 ctcctcggcg atctcgtcga gaatgggtgc gatcatcttg caggggccgc accactccgc
    14281 ccagtaatcc accaagacgg gcttgtcgga agagagcacg tcgttgtcga agtcggaatc
    14341 gctggtgtga acgatgttgt cgctcatcaa tttgctccag taatgatatg acagtcaccc
    14401 ggtctgggac gcgacgcttg gagagggccc agggccgtgg cggaacggtg gcagtttatc
    14461 acgacatcgg cgggcggcca ctaatccgcg agggctgcgg cgacggtgaa cgcgaagtag
    14521 gtcagaatgc cgtcggcgcc cgctcgtttc aggcagagca gcgactcggc gacgacggat
    14581 tggtcgagcc agccattgcc gacggcggcc atgtgcatcg catactcgcc gctgacctga
    14641 taggcgaagg tcggcacgcc aaaccggtcc ttgacccgtc ggatgatgtc caggtacggc
    14701 attcccggtt tgaccatcac catgtccgcc ccttcctcca ggtccagggc gatttcctgc
    14761 agggcctcgt cgctgtttgc cgggtccatc tggtaggtat gcttgctgcc ggtgccgagg
    14821 gtggccgacg agcccaccgc atcgcgaaag ggcccgtagt aggccgacgc gtactttgcc
    14881 gagtaggcca tgatgagggt gttgtcgtga ccctcgctct ccagcatctg ccgaatggcg
    14941 cctacccgtc cgtccatcat gtcggagggc gcgatgacgt ccgcgccggc gttggcgcag
    15001 acggctgcct gtcggcagag cgcctccacc gtgacgtcgt tgagcacgta tccgctttcg
    15061 tcgatgattc cgtcctggcc gtgggttgtg tagggatcaa gggccgcgtc ggtcatgacg
    15121 ccgagatcgg gtaaagcttc cttcagggcg gcgaccgccc gcgggatcag gccatccggg
    15181 ttccaggcct cgcaaccatc cggcgttctg agatccggct gcgtgtacgg gaacagagcg
    15241 atggcgggga tcccgaactt tgcggcttcc tccgcgtgcc tgagaaggtt gtcgatggac
    15301 agccggttga tgcccggcat ggaatccacc gctatctgct gatcggcgcc ctccacgacg
    15361 aagaccgggt agatgaggtc gttgaccgtc agctcggtct cgcgcacgag gcgccggatg
    15421 aagtcggtct gtcgattgcg tcgcaggcgc gtcgcgggga aactgcgcgg catgatgcgt
    15481 gtcctaactc ttgcgatgct ggctttctct gtgcctgggc ggctatggta atgcaacgcg
    15541 acgcggactc cagcgtatga gcggatccgg ttccggcgcc ccgggggcgg aatcccgcgg
    15601 ccgccatcga aagatcttgg tgcttgtgct cgtcgcaggt cttctcggcg cctttttcct
    15661 gaccggcctg aacgactatc tcagcctctc ctttctcaag ggcgttcacc acgatgccgt
    15721 cgttttcgtg agag
//
LOCUS       DBNHLM010000285         5722 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_880161, whole genome shotgun sequence.
ACCESSION   DBNHLM010000285 DBNHLM010000000
VERSION     DBNHLM010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5722)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_880161"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="AC16QF_13210"
     CDS             complement(<1..1105)
                     /locus_tag="AC16QF_13210"
                     /inference="COORDINATES: protein motif:HMM:NF015714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR6449565.1"
                     /translation="MLAGILLGVVRRGLPACGFLVGFAWAAFAMQQLLESRLPGCLDG
                     AEELMVVDVVSEPRRREDGAYTFSADVRSTRAGLCADVLGRRLRLTWYEAPGIGQGER
                     WRVLARLRTPWAYQNPGGFDFERWLLAEGLNGSGYVVSGTRIGGPRASWRAPWLEGVR
                     GYLETEHPAAAGVLYALVSGDGSLIASAGWRTLRDTGTVHLMVVSGLHVGLVAGMGYQ
                     IARLAVRALPLVLLWVPARRAGALGGVCAASGYVALTGGGVPGLRALCMSALVLGALA
                     WGRRLSPWLVWLVALALVLVGQPAVVHRQGLWLSFGAVAVLIWFFAGRSVREPRLAGV
                     AAALVRAQYALLLGLSPLLVMLQGQIPSSGPVAN"
     gene            1307..1816
                     /locus_tag="AC16QF_13215"
     CDS             1307..1816
                     /locus_tag="AC16QF_13215"
                     /inference="COORDINATES: protein motif:HMM:NF021363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HIR6449566.1"
                     /translation="MRKWLKEPEIWHLHRRSVAGAAFIGMFCAFLPVPFQMLIAAVLA
                     VASRCNLPMSVVLVWITNPLTIPPMFYFAYRLGAWLLNMEIEVATIELSWEWLKTHIG
                     RIGYPLIVGSLVCGWVSGLTAMVVVRIAWRMHVIKRWRERRRRRLERQMATDDSGGVT
                     PDEAPRAGS"
     gene            complement(1782..2990)
                     /locus_tag="AC16QF_13220"
     CDS             complement(1782..2990)
                     /locus_tag="AC16QF_13220"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR6449567.1"
                     /translation="MSTSLWIALRYLVTRRRQFAAFVTWVSVAGLALGVLVLTVVVSV
                     MNGFDAELRHRILGSVPHLIIEGESVTDPNVGSLLDRPEIVGSFDFFLGSGMVTRSGA
                     VHPVTVYGLDAEGLTGLGEIGQAMRSGRLSGLFEIPRGIVIGEPLAAHLGLLPGDSVA
                     VVISEPTASGVQPRFGRYELVGTFEVGAVIDYSVVVVARDALDGLRLDEVGVNGARLV
                     LADPLDAPRLAAELAMARPAWNLVSWTDSYGELFQAVGLEKMVMFIILLMVVAVAAFS
                     IVSGQMMAVADKRADIAILSTMGASRRTILGIFLLQGVLVSGLGIAVGLALGTLTAEH
                     IGAVIAGVEDLLGFRFLAGTYFVQVPSEVEVADLVIIGVMSSCLCLASAWLPAHRAAI
                     MNPLEGLHPG"
     gene            complement(2987..3661)
                     /locus_tag="AC16QF_13225"
     CDS             complement(2987..3661)
                     /locus_tag="AC16QF_13225"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6449568.1"
                     /translation="MSEPVLEARGLVKSFTQGTTRVVVLSGADLDVVAGERVAIVGRS
                     GSGKSTLLHLLAGLDDADAGSVIVGGKDMTAGSPAQRAHIRNAHMGFVYQFHHLLPEF
                     SALENVIMPLRLGGMDPAAAAERARGMLERVGLAERFEHRPSALSGGERQRVAVARAL
                     VGSPLVVLADEPTGNLDRDNAEHVFRMMCELSDEHGVGFVIVTHDEEMLGSMHRVLRL
                     ENGRLA"
     gene            complement(3654..4877)
                     /locus_tag="AC16QF_13230"
     CDS             complement(3654..4877)
                     /locus_tag="AC16QF_13230"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6449569.1"
                     /translation="MIKAVALSVGSRYAFSRRSTLSFISLVAISGLALSVAVLVIVTS
                     VVNGFDRELRERVFGVLPHLSFVGREPLAFSLDDLEALRAIPGVRGIAPFVEGAALVA
                     VADGVGAVMVTGMEPESYESVSDVHQFVRGRMPQAGEYGVLLGDQVAQRLGVAIGDAV
                     TLVLPSATVTPAGLFPRQKRFVITGLLDSQSDLDGRAAYVRLTVAQKLFRLGDRIHGY
                     QARLDDLFAALEVGSAGVLALGPQRLFARSWMRTHGNLYQAIGMLKSTLFVLLSFLIA
                     VAAFNLISTLVMVVDQRSGDIAVLRTLGSDTSTLIWTFVILGVLLGGAGIAAGLGLGA
                     ALALALPGLYEWLTTLLGADLLAQYFVTYLPVELQPGDLLGIAVTAFLLCVLSTLYPA
                     RRAAGLQPFKVLAYE"
     gene            complement(4958..5182)
                     /gene="nqrM"
                     /locus_tag="AC16QF_13235"
     CDS             complement(4958..5182)
                     /gene="nqrM"
                     /locus_tag="AC16QF_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HIR6449570.1"
                     /translation="MTILLTFLCMMAVVGLMAIGVMFGRRPIAGSCGGLKNLGIGGEC
                     EICGGDPARCESPGDTRLAVDATNRARRSR"
     gene            complement(5184..>5722)
                     /locus_tag="AC16QF_13240"
     CDS             complement(5184..>5722)
                     /locus_tag="AC16QF_13240"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF014477.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR6449571.1"
                     /translation="KSTDVYVDLSAIAKGFAVDRIAQRLFELECSDFLVDVGGEVRAS
                     GVNPQGAAWRIGVEVPDPDSFGAIQRVLRISGSGLATSGDYRNFLEVDGERFAHTIDP
                     RTGYPVDHTLASVTVVHESAMLADGYATLLNVLGPQEGFSLAEGLGLPVLFLIRTPNG
                     FEERYTVGLEQFLGQTDY"
BASE COUNT         1198 a         1992 c         1747 g          785 t
ORIGIN      
        1 ggttggccac cggtcccgat gagggaatct gtccctggag catgaccagc agcgggctga
       61 gccccagcag caacgcgtat tgagcgcgaa cgagagctgc agccacccca gcgagcctcg
      121 gttcccggac gctgcggcct gcgaagaacc agatcagcac cgccaccgcg ccgaaggaga
      181 gccagagtcc ctgccgatgg actaccgccg gttggccgac cagcaccagc gccagggcaa
      241 ccagccatac cagccagggc gacagccgcc tgccccacgc cagggccccc agaaccagcg
      301 cggacatgca cagtgcccgc aatccgggaa ccccaccgcc ggtcaatgca acgtatccgg
      361 atgcggcgca aacaccgcca agggctcccg cccggcgagc cggaacccac aggaggacca
      421 gcgggagtgc acgaacggct aaacgcgcaa tctgatagcc catgccggcc acgagtccaa
      481 cgtggagccc ggatacgacc atcagatgaa ccgtgcccgt atctcgcagg gtgcgccagc
      541 cggccgaggc gatgagcgag ccgtctccgc ttaccagggc gtagagcaca ccggcggccg
      601 ccggatgttc cgtttcgagg taacctcgca cgccttcgag ccacggggca cgccaggatg
      661 cgcgcgggcc gccgatccgc gtgcccgata cgacgtagcc gctcccgttc agcccttcgg
      721 caagcagcca gcgttcgaag tcgaaccctc cgggattctg atacgcccat ggcgtgcgca
      781 gccgcgccag aacccgccag cgctcgcctt gcccgatccc gggagcttcg taccaggtca
      841 gccgaagccg acggccgagg acgtcggcgc agaggccggc cctggtgctg cggacgtcgg
      901 ccgaaaaagt ataggccccg tcctcccgcc ggcgcggctc cgagacgaca tcgacgacca
      961 tgagctcctc ggctccgtcc aggcagccgg gcaggcggga ctccagcagc tgctgcatcg
     1021 caaacgccgc ccaggcgaac cccaccagaa acccgcaggc gggaagcccg cgccggacga
     1081 cgcccagcaa gattccggcc agcatgagcc acgccgtcgg gacaggcagt ggaagcgggg
     1141 cgacgggcgc ggcgagaacg aggtgaccca gaacgagccc aacaagccaa gcccgcatgt
     1201 gtgcaaggtt tccatcgggt gcataatttc cgttatgcca aaaaagtttc tccgcaagca
     1261 cctgccaaac ccgacggttc tcagtgagca tcgggcgctg cgccccgtgc gaaaatggct
     1321 caaggaaccg gaaatctggc acctgcaccg ccgttcggtt gccggcgcgg ctttcatcgg
     1381 catgttctgc gctttcctgc cagtgccgtt tcagatgctc atcgcggccg tgcttgccgt
     1441 ggcgtcacgt tgcaacctgc ccatgagcgt cgtcctggtc tggataacga acccgctgac
     1501 catcccaccc atgttctact tcgcctatcg gctgggcgca tggctgctga acatggaaat
     1561 cgaggtggcc acgatcgagc tcagctggga atggctcaaa acccacatcg gacgcatcgg
     1621 ctatccgctg attgtaggat ccctggtctg cgggtgggtc agcggcctca ccgccatggt
     1681 agtcgttcgc atcgcctggc ggatgcacgt catcaaacgg tggcgcgaaa gacggcggcg
     1741 gcgtctggag cgccagatgg caactgacga ctccggcggc gtcaccccgg atgaagcccc
     1801 tcgagcgggt tcatgatcgc ggcccggtgc gcgggcaacc aggccgaagc caggcaaagg
     1861 cacgacgaca tgacgccgat gatcaccaga tcggctacct cgacctctga gggcacctgg
     1921 acaaaatagg tgccggcgag aaagcgaaaa ccgagaagat cctcgacgcc tgcgatcacg
     1981 gcaccgatat gctcggccgt caaagtcccg agcgccaggc cgacggcgat gccaaggcca
     2041 gagaccaaaa ctccctgcag caggaagatg ccgaggatcg tccttcgcga agcgcccatc
     2101 gtgctcagaa tcgcaatgtc cgcgcgcttg tcggccaccg ccatcatctg ccccgatacg
     2161 atgctgaacg cggccaccgc aaccaccatg agcagaatga tgaacatcac catcttctcc
     2221 aggcccaccg cctgaaagag ctcgccgtag ctgtccgtcc agctgaccag attccatgca
     2281 ggccgagcca tcgccagctc cgctgcgagc cgcggcgcat cgaggggatc cgccagaacc
     2341 aggcgcgccc cgttgacgcc cacctcgtcc aaacgcaagc cgtcaagcgc gtcgcgggca
     2401 acaacgacca ccgagtagtc gatgacggcg ccgacctcga aagtaccgac cagctcgtag
     2461 cgaccaaagc gtggctgcac gccggacgcc gtcggctcgg agatcaccac agccaccgaa
     2521 tcgcctggca gcaggcccag gtgcgccgcc agcggctcac caatcacgat tccccgcggg
     2581 atctcgaaca ggccggacaa acgtccgctg cgcatggctt gaccgatctc tccaagcccg
     2641 gtgagtcctt ctgcgtccag gccgtagacg gtcaccggat gaaccgcgcc gcttcgggtg
     2701 accattcccg aacccaagaa gaagtcgaaa gagccgacga tttccggccg gtcgagcagc
     2761 gaaccgacat tcgggtcggt gacgctctcc ccttcgatga tgagatgggg cacggatccg
     2821 agaatgcggt gcctcaactc ggcatcgaac ccgttcatca cgctgacgac caccgtcagc
     2881 accagaacgc ccagcgccag accggcaacg gagacccacg tgacgaaagc ggcaaactgg
     2941 cggcgccgcg tgaccagata gcgcagagca atccagagcg acgtgctcac gccagccggc
     3001 cgttttccag acgcaggact cggtgcatgc tgcccagcat ctcttcgtcg tgggtgacga
     3061 tgacaaaacc cacaccatgc tcatcgctga gctcgcacat catgcggaaa acgtgctccg
     3121 cgttgtcccg gtcgaggttc cccgtcggct cgtcggcgag caccaccagc ggactgccca
     3181 ccagcgcgcg ggcaacggcc acccgctggc gctctccgcc cgacagagct gatggccgat
     3241 gctcgaaccg ctcggccaga cccactcgct ccagcatacc gcgcgctctc tctgcggcgg
     3301 cggcagggtc catgcctccg agccgcagag gcatgatgac gttttccaga gccgagaact
     3361 ccggcagcag atggtgaaac tggtagacga agcccatgtg cgcgttgcgg atgtgcgccc
     3421 gctgcgcggg agatcccgcc gtcatgtcct tgccgccgac gatcaccgac cccgcgtcgg
     3481 catcgtcgag acctgccagc agatgcaaca gggtgctctt gcccgagccg gaccggccga
     3541 cgatggcgac ccgctcgccg gcgacaacgt cgagatccgc ccccgacaac accacgaccc
     3601 gggtcgtgcc ctgagtgaac gacttgacga ggccgcgggc ttcgaggacc ggctcactca
     3661 tacgcaagaa ccttgaaagg ctgtaacccc gccgcgcgcc gcgccgggta cagggtgctc
     3721 aaaacgcaca gcaaaaaggc cgtcacggcg atgccgagaa gatcgcccgg ctgcagctcg
     3781 accggcaggt aggtaacgaa gtactgggcc agcagatcgg ctcccagaag cgtcgtcagc
     3841 cactcataca ggccgggaag agccagagcc agcgcggcgc ccaggccgag acccgcggcg
     3901 atcccggcac cgccgagcag aacgccgaga atgacgaacg tccagatcag cgtcgaggtg
     3961 tcactgccca gcgtccgcag caccgcaatg tcgccgcttc gctggtccac caccatcacc
     4021 agggtcgaga tcaggttgaa cgcggccacc gcgatcagaa acgaaagcag gacgaacagc
     4081 gtgctcttca acatgccgat ggcctgatag aggttgccat gggtacgcat ccaggaacgc
     4141 gcgaacaggc gctgcggacc gagggcgaga acacctgccg aacccacctc caacgcagcg
     4201 aacagatcgt ccagcctggc ttgatacccg tgtatgcgat cgccaagccg gaacagcttc
     4261 tgcgccacgg tcaggcgcac ataggccgcg cgaccgtcca ggtcggactg cgagtcgagc
     4321 agccccgtaa tcacaaagcg tttctgccgc gggaacagcc ccgcaggcgt gaccgtcgcc
     4381 gacggcagca ccagggtgac agcatcgcca atggccacgc cgagccgctg ggccacctga
     4441 tcaccgagca ataccccgta ctcgccggct tgaggcatgc ggccgcgaac aaactgatgc
     4501 acgtcggaga cgctctcgta gctttccggc tccatgcccg tgaccatcac ggcgcccacc
     4561 ccgtcggcca ccgcgaccag cgcggcgcct tcaacgaatg gggcgatccc ccggacgccg
     4621 ggaatggccc gaagcgcctc cagatcatcg aggctgaatg ccaacggctc gcgcccgacg
     4681 aagctcagat gaggaagcac gccgaaaacc cgctcgcgga gttctctgtc gaagccgttc
     4741 acgaccgacg tcacgatcac cagcacggca acgctgaggg cgagcccgct gatggcgacc
     4801 aggctgatga aggaaagcgt gctccgtctc gagaaggcgt agcgactgcc cacgctgagt
     4861 gcaactgctt tgatcaatgc aaaccgaccc ggcccggggg aggccgcatg ctaacacgtc
     4921 ggtagcgccg cgcgccctcg gggagaagcc ctggtaatca gcgggagcgt cgtgcccggt
     4981 tggttgcgtc cacggcaaga cgggtgtccc caggcgattc gcagcgcgcc gggtcgccgc
     5041 cgcagatctc gcactcgccg ccgatcccca ggttcttcag cccaccacag ctgcccgcga
     5101 tgggccggcg cccgaacatg acgccgatgg ccatgagccc caccaccgcc atcatgcaga
     5161 gaaaggtcag aagaatcgtc acgtcaataa tccgtttgcc cgagaaactg ctccaagccg
     5221 acagtatacc tctcttcgaa gccgttcgga gttcggatca ggaaaagtac cggtaatccc
     5281 agcccctcgg ccagggaaaa gccctcttgc ggccccagga cgttgagaag cgtggcgtaa
     5341 ccgtccgcca gcatggcgga ttcatgaacc acggtcaccg aggcgagggt gtggtcgacc
     5401 ggatagcccg tgcggggatc gatggtgtga gcaaagcgct cgccgtcgac ctctagaaaa
     5461 ttgcgatagt cgccggaagt ggcaaggcca gacccgctga tccgaagcac ccgctgaatc
     5521 gcgccgaagc tgtccgggtc cgggacctcc acgccaattc gccaggccgc gccctgaggg
     5581 ttcacgccgg aggcgcgaac ctcgccgcca acgtcgacga gaaaatcgga acattcaagc
     5641 tcgaagagcc tttgcgcaat ccgatcgacg gcgaacccct tggcgatggc agacagatcg
     5701 acgtaaacgt cggtggattt gc
//
LOCUS       DBNHLM010000286        29957 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_460625, whole genome shotgun sequence.
ACCESSION   DBNHLM010000286 DBNHLM010000000
VERSION     DBNHLM010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 29957)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 29957)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29957
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_460625"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..549
                     /locus_tag="AC16QF_13245"
     CDS             193..549
                     /locus_tag="AC16QF_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6449572.1"
                     /translation="MKYVRIYSGDDGQSHFEDVEVDLADHGPLGRISDLVRGKGVVFR
                     EVEGEYHYDFHNAPRRQFVVNLTGSVDIEVGDGTVRRMEPGSILLAEDLTGQGHVSRS
                     VNGEPRTCLFIPLADD"
     gene            577..1473
                     /gene="rarD"
                     /locus_tag="AC16QF_13250"
     CDS             577..1473
                     /gene="rarD"
                     /locus_tag="AC16QF_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HIR6449573.1"
                     /translation="MSEQRAGMLFALAAYGFWGFAPVYFKVLDFAGPVEIIAHRVLWS
                     VPLLALLIGARRQFGALLALRADQVGWLGLSGLLVAVNWGVFVWALLNGRMLETSLGY
                     YINPLVSVLLGVLFLGEWLRRGQLVAVALAAIGVVNEVVSVEVIPWAGLTLAVSFGFY
                     GLVRKRLGVDSAVGLGVEATLLLPVALGYLAYQFEAGQGALNRGEGGEIAWLAASGLV
                     TTFPLVCFAAAALKLPLSTLGFVQYLAPTITFFLAVLVFGEAFRWEQSVTFGCIWLAV
                     VIFSAEGVYHQRRLQRRLGRWA"
     gene            complement(1479..2177)
                     /locus_tag="AC16QF_13255"
     CDS             complement(1479..2177)
                     /locus_tag="AC16QF_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449574.1"
                     /translation="MGKVRAMLRLPIMLLFALLVACGRGGILQGGKLEIPQSTGNWFC
                     QPGTGGDEWDCIQDEELARSPQLAAATTPAPPVEESGEGPEAEPPEPAATESPVPRDV
                     EPGAVQEQPPIEEPSAADPEPPKAAEPELDADGLMGLPDDYFAVQLVALDSAAELSAF
                     VDTHGLGYLPHARVERDGRIHHVLLLGFYESYALAEAASLDPPAPIEPGDAWIRPLAG
                     LKRAVERAVSLDAG"
     gene            2397..2987
                     /locus_tag="AC16QF_13260"
     CDS             2397..2987
                     /locus_tag="AC16QF_13260"
                     /inference="COORDINATES: protein motif:HMM:NF025637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR6449575.1"
                     /translation="MIATLPEFDRAPRVLRLNIAGHPVDWVSWQEAVCLYARDIVIWT
                     LGDPILRIRGGHSRLDSSQSSVEIHAIIACDGRVTRPSRSVPPLTNEALFRRDLNVCL
                     YCGGTFADGALTRDHVVPTSRGGRDVWDNCVAACRRCNHHKGSRLAEECGMELLALPY
                     VPNFAEYLALINSGRILGDQMDFLRKSFGSNSRLLQ"
     gene            complement(3004..4437)
                     /locus_tag="AC16QF_13265"
     CDS             complement(3004..4437)
                     /locus_tag="AC16QF_13265"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR6449576.1"
                     /translation="MSDAIAPFVNELWDRSIIPTLSDYVRIPNKSPAFDADWEAHGYM
                     QAAVELLRGWCSVPDIGGLTTEIVTIPGRTPVLFCEVPATAPDAGTVLLYGHYDKQPE
                     FDGWEPGLGPWEPVLRDGKLYGRGGADDGYALFGSLAAIAALQAEGIPHGRCVLLIEG
                     CEESGSFDLPAYVELLEERIGQPDLVICLDAECGNYEQLWTTTSLRGMLPGVLTVKVL
                     SEGQHSGAAGGIVPSSFRILRALYDRVEDADTGSLHDCLYVPIPDDARRQLADVAQVL
                     GATTIDRFPWLDGVTPDGDDLQDLLLANAWEPSLATVGLAGAPDVGNAGNTLRPGTSA
                     KLVFRLPPTLDADEAARTIKAELERSPPNGAHVRFELETPQTGWCAPPVAPWLESALM
                     RASQQYFGKPAMYMGMGGTIPFMKMLGDSFPGVQFVVTGVLGPRSNAHGPNEFLEIAT
                     GKRITACVAAVLAEHARRQARRQARQN"
     gene            complement(4434..6281)
                     /locus_tag="AC16QF_13270"
     CDS             complement(4434..6281)
                     /locus_tag="AC16QF_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6449577.1"
                     /translation="MPMVLATVLALLAPSLSFAEPDPELETITVTAHRIRTDRPVLPV
                     LTRTTGTGPAVGADALRSLPSLAISQAGSLGSVTQVRVRGSEANHLMVLMDGVELADP
                     ATDASFNFANLSLTGTTRVELLAGAHSAIWGSDAVAGVLNFITKPTERRRRLSLEGGS
                     FDTRQAALQLADTTGSYHYNLALSEFRTDGTNIATTGEEDDGFDQSSYRLSGGYRSES
                     WTIEALVHHLKSDADFDPIPFPAFQPRDGDRVSKHGEYLGSVAATWHGDERFEQQIRL
                     SYFDTDNRSFADGARESRTAAARFKATSLTRLRASSRHAVTLMLEHEKERFEQRGAPS
                     IFGDPNQNQSVRTNSLALEYLWAPLDDLSLALSARTDDNSDFENKESYRAAARYRLTG
                     AVELWANWGTGIKNPSFVERFGFTPDTFLGNPDLDPETNRHLSAGAVIGHRQWTLRAT
                     AFRDRLEDEINGFYFDGAAGAFTSINEPGTSKRDGVEVETTLAIPTGVLSMGASWLDA
                     AEPDAAREIRRPQWQAYATVSHERGPFSAELSGYYVGNREDLDFSGFPAERVRLGDYV
                     LLRAHLAYRLGRHLKLALRGENLLDESYQDQLGYASPGRAVYLQVAIDL"
     regulatory      complement(6322..6531)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(6534..7676)
                     /locus_tag="AC16QF_13275"
     CDS             complement(6534..7676)
                     /locus_tag="AC16QF_13275"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6449578.1"
                     /translation="MGAGIGGLTLAAALGEAGFRDVTVYDRGEPGSPEPSDLVLSPNG
                     TRVLRALHLGPFLSRAHKPDAYVQRSWRSGFVLAHRPLGAFMEARYGAPEVVVARQAL
                     LEALATRCAHHGVAVRTGQPCARIAQDARSVTLTFGSQEICHDVVIGCDGEASAVRAG
                     MGYAETTQTTQDVVWTGTCPGTDLPIGLRANAVTTWLGPRAYLTQWPSADGCAARFLA
                     FARNPGAGAKLAGAFRNWHEDVFRLVDSATGVTRSAPVEREPIAQWYDRRVALLGDAC
                     HPVTPHLQQGASLAMEDAWVLSRMLERWEDEPAKGFPDYERYRKVRLGRFRQGQRHHL
                     AELTTDPGGKAWRRNLRLSLTSRFLPEIAMQRMDWLYGYDCIKGFE"
     gene            complement(7696..9093)
                     /gene="cysS"
                     /locus_tag="AC16QF_13280"
     CDS             complement(7696..9093)
                     /gene="cysS"
                     /locus_tag="AC16QF_13280"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR6449579.1"
                     /translation="MDIRLHNTKSGRKEAFDPVDPQRITMYVCGPTVYGLVHIGNGRP
                     AVVFDVLYRLLRAAHPEVVYARNITDIDDKINAAARDNDEPIQALADRFSSAYREDVA
                     ALGVLEPTVEPRATHHIAEIIAMIETLIAKGNAYEAQGHVLFHVPSDPEYGSLSHRSL
                     EDMLDGARVEVAPYKQDPKDFVLWKPSTTDLPGWDSPWGRGRPGWHIECSAMISKHLG
                     QTIDIHGGGSDLAFPHHENEAAQSRCANDHPEYVRYWLHNGMLTMGQEKMSKSVGNIV
                     TIRDLLDRFPGEVLRYALLSGQYRSQLAWSEDLLDQAGSSLERLYQALRDAGVEDETS
                     SDYRDLPAEEYPTVVVAALADDLNTPQALAAMHQLASAIHRTSDASEKVELARRLLAG
                     GWLLGILTESAKAHFTAGRDIDAAEIDRLIEERNAARKAKDFARADEIREELTDLGIE
                     LEDTRDGTRWKLRDG"
     gene            complement(9112..9513)
                     /gene="hisI"
                     /locus_tag="AC16QF_13285"
     CDS             complement(9112..9513)
                     /gene="hisI"
                     /locus_tag="AC16QF_13285"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015637550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HIR6449580.1"
                     /translation="MFEYLEDAELGHEVALDALLDAVKFDSDGLVPAIAQDADSRDVL
                     MLAWMNREAVERTLKDGYACYWSRSRGAFWRKGESSGHLQALKEMRFDCDGDAILLIV
                     DQTGPACHTNRPSCFYLSVSDGTARVSSEPV"
     gene            complement(9515..10879)
                     /locus_tag="AC16QF_13290"
     CDS             complement(9515..10879)
                     /locus_tag="AC16QF_13290"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013079.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HIR6449581.1"
                     /translation="MAEALIVSSDAAATPLRLIRTSDFEAFASELSEAERSWIRRAQF
                     AAKSGEFAWLPGADGHPAELVAGWDGGDDLASLGGLPTRIPEGVYELKTEVSDLQLLG
                     WALGSYQFVRYRERDRAPAQLLIPARNDADLVANMDAATTLVRDLINIPAGDMLPSHL
                     ADEAIQVADANQANCRVLVGEQLLEGGFDAIHAVGRAAADAPRLIDITWGDPDHPLIV
                     LVGKGVCFDSGGLDIKPAASMRLMKKDMGGAANALGLAQLIMASNLPVRLRVLIAAVE
                     NAVGANAYRPGDVVPTYKGISVEIDNTDAEGRMVMCDCLALGAEDEPALMVDFATLTG
                     SARSAVGAEIAAMFCNDDEVAAGLSAHGDRWDDPVWRLPLHKPYAYLLDSKVADTVNS
                     ASSPFAGSITAALFLEKFVGSAPWVHFDIMAFNTRPRPGRPEGAEAMAIRAVFGYLQE
                     RFGG"
     gene            10948..11508
                     /locus_tag="AC16QF_13295"
     CDS             10948..11508
                     /locus_tag="AC16QF_13295"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6449582.1"
                     /translation="MLTEVTTHHLELTDPDQFKPAATGPAYALKQVQRPTAELNRFLY
                     STVGAAWFWYERLSWSRARWLEFLDRPEVVTWVAYVDGSPAGYFELEDQPGGSVEICY
                     FGLLPAYIGQGMGGALLTDAIRAAWARGASRVWLHTCSLDHPFALANYLARGFSLFDI
                     VTTTKNLPDPMPEPRPGDAEAGRSSD"
     gene            complement(11500..12492)
                     /gene="lipA"
                     /locus_tag="AC16QF_13300"
     CDS             complement(11500..12492)
                     /gene="lipA"
                     /locus_tag="AC16QF_13300"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR6449583.1"
                     /translation="MSKPVVSGKKFRTPEGFTAIKDGMKANGQAEPATGRKPPWLRVR
                     LGGGEKYQAVRRTVATHRLATVCEESHCPNIGECWNNGTATIMLMGSVCTRACRFCAV
                     DTGNPGGRLDPDEPANAAESVRLMGLRYVVLTSVDRDDLADGGAGHYAKCIQAIKAVN
                     PETAVEALTPDFSGSAEAVAVVVGSGLDVFAQNLETVERLTGDVRDPRAGYDQTLGVL
                     AEAKQVRRDLLTKSSLMLGLGEQDDEICQAMDDLREHGVDILTLGQYLRPTRNHLAVA
                     RWVHPDEFERYREWGLERGFTEVASGPLVRSSYRADRILEGNNLGLGRIPVKSI"
     gene            12541..13977
                     /locus_tag="AC16QF_13305"
     CDS             12541..13977
                     /locus_tag="AC16QF_13305"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6449584.1"
                     /translation="MQERPAVTRRVRWLYGAGAAAYGVKENGFSYFLLFYYNQVLDLP
                     GAYAGTAILIAMIFDAISDPLVGVWSDNTRSRWGRRHPFMYSAALPVAVIYFFLWNPP
                     ELTEFQLFVYLTVGCVLVRFFVTLYEIPSSAIVAELTDDYDERTRLLSYRYMMGWFGG
                     LGMAVLNWGLFMVVWGEAGETTFRVYGAVGAVIMFVAIVGSSLGLHRYIPYLHPPPER
                     TSYRVRQLTGDLIETISNRNFAALFFAGLFAAVGAGVATNFDTYIITHFWEFEAEQWR
                     WVIISLFVSALIPMYLAPLVTRRWDKKRSALGIYAFQIVFAALPILLRLAGWFPDNDS
                     PWTYPLIWAHAVVNVVVIVMFGVIQSSMLADVVEHSQASTGRREEGLFFAARSFAQKA
                     TSGVGAFIAGLALDWIAFPRGATPGTVPDAVIWDLGFIYGPALMIFYVLALVSIGFYR
                     ISRQGHDQRVGALRAGATAPSGSMEESR"
     gene            13974..14696
                     /locus_tag="AC16QF_13310"
     CDS             13974..14696
                     /locus_tag="AC16QF_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019689442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6449585.1"
                     /translation="MNDRPVIVTGGAGGIGSAIVARLVADGHPVAVLDLHPASATPSG
                     SLFSYPCDIADLDAVERAVAEFEAAVGAPFGLVNNAGWDQALPFLETGPDLWEKVIDV
                     NLRGPVNVTYAVLRRLAGAGRGRVVSIASDAGRVGSSGEAVYSACKGGIIAFMKTLAR
                     EHARQGITFNSVCPGPTDTPLLDSFDESGRLAQALERAIPMRRLGRPEDFPGIVAFLL
                     SDEASFITGQTVSVSGGLSMQG"
     gene            complement(14698..16575)
                     /locus_tag="AC16QF_13315"
     CDS             complement(14698..16575)
                     /locus_tag="AC16QF_13315"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6449586.1"
                     /translation="MTAASQPGAPERRNYSMFDAEISGAVLNLALLRRLLKWLRPYRV
                     TFGISAVLVLLASTLQVLMPIIISLVVIDHLIRGETDSLAPDLGMVELTEWLADILAL
                     HPLMAACLLYAGLQIAWAAAGHAHRMTLISSVINGLRDLRLDLFRHLETRPSSFYDRV
                     AVGRVMTRVTNDIEALYELLRGLGSLIGEFMPFFVALAIMLAIDVELTLLLLLALPVM
                     GSVTFYFRRATRRLFRMVRMSLSALNQNMQENLAGLQVVQLSERQQYNLDRYTKINEE
                     NRGHELRTTRFETLYGAFNDSMAHLALGVIIWYAGGAAVQEEMTLGAVILFTRFIDML
                     FHPIVVLGEQTNILFRAMASGERIFQALDWDEKIHEPADPVTLPPRLQGEVTFRHLNF
                     AYDPDVQILHDVSFEIGAGEKLAIVGPTGSGKSTLIRLLGRFYEFDDGQIFLDGIDLN
                     RIHTRDLRQRIGVVLQDFHIFSGTVFDNIALGDPRITRERAEEAARRVNAHGFISDLP
                     RGYDTELSERGQNLSQGQRQLLAFARVLAADPEILVLDEATASIDTETELLIQDALRK
                     LTAGRTSIIIAHRLQTIQEADRVLVLQGGRVVELGTHEELIASEGLYHTLHSLQFQDE
                     G"
     gene            complement(16572..18332)
                     /locus_tag="AC16QF_13320"
     CDS             complement(16572..18332)
                     /locus_tag="AC16QF_13320"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6449587.1"
                     /translation="MIRSLIRLKSFLAPYRLRLGAGVGAFGIARFFEAMVPIFVAIGI
                     DRMSAGNADMTTPLLGIAGAVIGRYVVVTYARYSVRCVGQHVAFDLRQALYESLQQQG
                     VRFFTRYTIGDMMTRAVADIGLIQRLIAMGTILMVILVYATLVGFGFMLYLSPSLTLL
                     LLPPLPLVFFYASWSARQMGVASRDVQNRLSDLGAHAQENLSGIRTIQAMVQEENEIR
                     RFGRTNQQYANAFYRQSQINSLMAAWMPSLAAICSVTILGYGGYLVLDGAMTVGELVA
                     FFMYVNMVVQPFRVAGFIVNLFQRAAVASDRLFEVITLSPEIADDPSGTTPATIRGDV
                     EIRHLTFAYDEGIRPALDDVSLTVARGESVTIMGRVGSGKTTLLKQLVRLLDTPPGAV
                     LIDGHDVRDYPLAQLRSQVAMVPQDAFLFGEPLGDNLTYDDPGRAVEKIWEAATSADL
                     KETIEEFPEQLDTIVGERGVTLSGGQKQRATLARGLIRGAPVLILDDCLSSVDTETEE
                     HILRELRRLRRRQTTILVSHRVSTARHSDRIVVMDAGRIVESGTHEELLARAGQYAEL
                     ERIQREGAVDTDYDLIGATT"
     gene            complement(18357..19748)
                     /gene="asnS"
                     /locus_tag="AC16QF_13325"
     CDS             complement(18357..19748)
                     /gene="asnS"
                     /locus_tag="AC16QF_13325"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003037.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HIR6449588.1"
                     /translation="MTDTIKDILAHRLPVGAEVTIEGWVRSRRTSKGGFSFVHVSDGS
                     CFDTIQAVADSQLANYEHDVEHLGTGCSVRIRGTVVESQGRGQDREIQATEVVVVGWV
                     DDPETYPIAKKRHTFEYLREVAHLRPRTNTFGAIARVRNAIAQAVHGYFNDNGFVWVN
                     TPIITASDCEGAGQLFRVSTLDIANRALTRGDSPFDDDFFGRETFLTVSGQLNLESYC
                     LALSKVYTFGPTFRAENSNTSRHLAEFWMIEPEIAFADLNANAELAEDFLKAVCRRVL
                     DACPDDMSFFADRVDEQAISRLEHVIATPFERIDYTDAVSILEKAGQNFEYPVQWGLD
                     LQSEHERYLTEVHVGGPVVVVNYPKTIKAFYMRLNDDDATVAAMDVLVPGVGEIIGGS
                     QREERLDVLDARMAEQDIGEDLWWYRDLRRYGTAPHAGFGLGLERLVLYMTGMDNIRD
                     AIPFPRVPHNASF"
     gene            19846..20622
                     /locus_tag="AC16QF_13330"
     CDS             19846..20622
                     /locus_tag="AC16QF_13330"
                     /inference="COORDINATES: protein motif:HMM:NF022665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HIR6449589.1"
                     /translation="MRNLIIVIALVLVLLGGLWLASDRSAGDREVPLPGAEPVPVPGP
                     RPAEPARRPQPMPVAEPAIVLPDLDASDPFLRERLAGVELPQNWIDRDDLVRRLAVVI
                     ENAARGEYPRRQLGFLAPSGRFRVVERQGRLFVDPDNYRRFDGYLDVLEGLDPRTAAE
                     LLSLIEPLLAVAMGELGSDGSVSDQIDAAVAEVMAVPDVVGEVELAQPKVLYEYADPA
                     LEALSPLQKQVLRMGPENAARLKRYVAEMAVALQPDPSDG"
     gene            complement(20648..21856)
                     /locus_tag="AC16QF_13335"
     CDS             complement(20648..21856)
                     /locus_tag="AC16QF_13335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6449590.1"
                     /translation="MTSRTARILHSIDEIPAEDWNRCAMPGGEIPHDPFLRHEFLAAL
                     EHSGSAAARTGWQPFHLVLEEADRILGVVPMYVKNHSQGEYVFDYAWADAWHRAGGDY
                     YPKLQVSVPFTPATGRRVLAAGQDEVSRPEIERALLGACAQVAAEIKVSSLHVTFLPE
                     AQWQIAGELGFLRRMDQQFHWHNAGYGSFEEFLGDLSSKKRKNLRRERRDALATGIRI
                     EWLTGSDIQESHWDAFYRFYMDTGSRKWGSPYLTRQFFSLVTETMAEDVLLIMCSRDG
                     RYIAGALNFIGGEALFGRNWGCIEHHPFLHFEACYYQAIDFAIERGLKRVEAGAQGPH
                     KVARGYLPCPTYSAHWIADAGFRNAVARYLDEEREHVREDIEYIEERSPFKSTVSLDK
                     FHNTRTLDIG"
     gene            complement(21923..22384)
                     /locus_tag="AC16QF_13340"
     CDS             complement(21923..22384)
                     /locus_tag="AC16QF_13340"
                     /inference="COORDINATES: protein motif:HMM:NF015325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR6449591.1"
                     /translation="MKHLLIVYHTQTGRTGQLAAAVHRGATNELVSDVDVRLLTAQNA
                     GADDLLWADGLLLGTPENFGYMSGGMKDFLDRTFYPVEGKILSLPYAIFISAGNDGSG
                     ALRAIRRIANGYPFKEVQEPVIARGEITSDHIDRCEELGLTLAAGLEAGIF"
     gene            22453..23658
                     /locus_tag="AC16QF_13345"
     CDS             22453..23658
                     /locus_tag="AC16QF_13345"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF005600.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR6449592.1"
                     /translation="MTQLDDITQARQRICGQIELTACSRSATLSAMTGADVHLKFENL
                     QFTASFKERGALNRLLQLTGEQQRFGVVAMSAGNHAQALAYHGKRLGVPTCIVMPRFT
                     PNAKVEQTRVHGAEVLLHGAEFDETLAFTEALAEERGLTLIHPFDDPAVIAGQGTLGL
                     ELVEQVPGLEAVLVPVGGGGLIAGVATAVKSVKPEVELLGVQVERFASVYAAFHGREV
                     PPGPPATVAEGIAVRAPGKNTLPLIRRHVDDVVLVSEEQVEQSIFKLLEIEKTVVEGA
                     GATSLAALLAEPGRFAGRRVACVLSGGNIDMMMLSSVVQRGLVRSRRLVRLSVEIPDV
                     PGALSELTRVLGALDSNIVDIAHQRAFGGSSVRATRVDLVLQMRGEEQVDEVVQELQA
                     RGYECARGG"
     gene            23735..24289
                     /locus_tag="AC16QF_13350"
     CDS             23735..24289
                     /locus_tag="AC16QF_13350"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6449593.1"
                     /translation="MRVNEPRIPALEQSEWSEAAQEIMGPMVAQDRVFNIFRTLSNHP
                     DLMRRWLVFANHILGKSTLPVRERELVILRIGYLCQSGYEWGQHVRIAQEAGMTAQEI
                     RSAKTGPDTPGLSDLDRLLLKATDELHADAHVSDETWKALTGHLNTQQLMDLVFTVGQ
                     YNMVSMVLNSFGVQLDEGLPGWDI"
     gene            24642..25049
                     /locus_tag="AC16QF_13355"
     CDS             24642..25049
                     /locus_tag="AC16QF_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449594.1"
                     /translation="MLYLNGAIGCIFIVIALLHAPHPDTFSWAPYLGAALLALISCRR
                     EVSMGVARALAVLTMVAMFYFFAAFFVHVPRLAADWYMHQEGWSAVCEILAAFAMIPI
                     LSDFSCRLKAECREARAARRAFFSVPSNIQPHR"
     gene            25062..25922
                     /locus_tag="AC16QF_13360"
     CDS             25062..25922
                     /locus_tag="AC16QF_13360"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR6449595.1"
                     /translation="MQFRPRRSVLYMPGANARAMEKARELPCDAIIFDLEDAVAVDAK
                     VAARDQVAQAVSAGGYGYRELIVRVNGLDTEWGRDDVAAAARLPVDALLFPKVESPEQ
                     VATIVAAVDAAGGKALPVWLMIETPAGVLDAPAVACGSPRVRCLVMGTSDLVKELRGR
                     HTRTRSNLNFALQRCVLSARSAGVDILDGVHLDFRNAETFRDACEQGRDMGFDGKTLI
                     HPTQIEAANEAFGYSAADVDHARAVIAAWESAQSEGKGVAVLDGQLVENLHAAEAQRV
                     LAFAAAVGDR"
     gene            complement(25956..26903)
                     /locus_tag="AC16QF_13365"
     CDS             complement(25956..26903)
                     /locus_tag="AC16QF_13365"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6449596.1"
                     /translation="MSISLGVHAGQQNMAMNDMRALWRKLDDKGVDWISAWDHFYEAP
                     PKGGTEPHFEALTTLGALAAETSNARIGCLVFYVGYRNPALLAKAATTLDHITSGRFE
                     LGIGAGWHVWEASAYGYPFPDIGTRLDMLEEAAQIIRGMLTQDRTSFSGKHYQVDDAS
                     CLPKPVQERLPIWIGGVGEKRTLKLVADYADGWNAAYLSPEEFARVNEVLNHWCEAEA
                     RDPASLRRAANVMFNLGMDARDLERQREALQRDWGAMADRVADGALLCTPEAAVDRIM
                     EYVNAGADEINIALRAPWNEEALDAYLTDIMPKVRALCE"
     gene            complement(27311..>29957)
                     /locus_tag="AC16QF_13370"
     CDS             complement(27311..>29957)
                     /locus_tag="AC16QF_13370"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6449597.1"
                     /translation="AELRGHREEMLEAVIDPAALEAYAISNEQLITTIVRNNRLIPAG
                     SIDTGEGRFAVKIPSIIERAEDVLDLPVRASGDTVVTLDDVAVVRRTFKDRTSYARVN
                     GNAALSLQITKRANANIIDTIDAVKAVVERHRPDLPNRVDVFFSQDQAPFAEGQVTEL
                     QGNILTALALVMVLVVAAMGFRSGVIVGLAIPVSFLFSLIFIYLLGYTFNFMVMFGML
                     LGLGMLIDGAIVVTEYADRKMTEGFERRAAYAMAAKRMFWPVTASVATTLAAFLPLMF
                     WPGIPGKFMSYLPVTVLTVLSGSLIYALIFGPVLGSLFGKAGARDAKAQRTLNELEDG
                     DPTRLKSVTGWYARLLTITSRHALATLSGILVILVTIFWGYGAYGKGMVFFSDSDPQF
                     AQVAVRARGNLSADEIDALVREVDSEILEVSGIRTVNTYTLLSGGSTRGGFDRVGSMF
                     LELHPESERDRKGAEIFAEIRQRTAGLAGIAVEVQKLEQGPPVGKPVQIQFSSYDREL
                     LEPAVTRVREYMETLPGLLDIDDTRSLPGIEWKLSVDRAQAALYGADVSQVGIAVQLV
                     TNGVKVGEYRPERADDAVDIRVRYPSDARGISALDDLKISTTKGLIPISNFVKREPAP
                     NVDTFQRIDGVPVEFIRANVAEGVLADDQVTLIRDWLSAQTFDPDLDIDFRGANEEQA
                     QSLAFVQVAFGLSLLLMFVLLVTQFNSIYQSALILVAVVMSTAGVLLGLLILQAPFSA
                     ILTGVGIVALAGIVVNNNIVLIDTYNHLRRQHPDLDHVTLIVRTGAQRLRPVMLTTVT
                     TIFGLLPLASNLSIDLVNRSIIYGGQLSGFWVPLSQAIVSGLTFATLLTLIATPALLA
                     LPHQLKDLAAQLRALVSRTRQPTGA"
BASE COUNT         4836 a         9415 c        10214 g         5492 t
ORIGIN      
        1 tgtccgcggt agaggttcgt ttcgatccgc tcgacgttca ggatgtcgac gatctctcga
       61 agcacgtggt tgctggtctg atccatggtc gggtcgatgg ctgcgccgca cggttggtgg
      121 cattctactg gacatggcac ccgatgagat gcccggatcg gggtcgggtg gggacactac
      181 ggaggcagcg acgtgaaata cgtgaggatc tacagcgggg acgacggcca atcgcatttc
      241 gaggacgtag aggtggatct cgccgatcac gggcccctgg gcaggatctc ggatctggtg
      301 cgcggcaagg gggtggtgtt ccgcgaagtc gaaggcgagt accactacga ttttcacaat
      361 gctccgcgcc ggcagttcgt ggtcaacctg acggggtccg tggatataga agtcggtgat
      421 ggcaccgttc ggcgcatgga gcccggcagc atcctgctcg ccgaagatct caccgggcag
      481 gggcacgtca gccgtagcgt caatggcgag cctcgcacct gtcttttcat accgcttgca
      541 gacgactgaa tcgtctctgc cattccggaa accgacatga gcgagcaacg cgcgggaatg
      601 ctgttcgcgc tggccgctta cggcttctgg ggctttgccc ccgtttactt caaggttctg
      661 gattttgccg ggccggtgga gatcatcgcg cacagggtcc tctggtcggt tcctctgctc
      721 gcgctgctga tcggcgcgcg gcgccagttc ggcgcgctgc tcgcgcttcg tgcggatcag
      781 gttggctggc ttgggttgag cggcctgctc gtggccgtga actggggcgt gttcgtctgg
      841 gctctgctca acgggcgcat gctcgaaacc agccttgggt actacatcaa cccgctggtc
      901 tccgttctgc tcggggtgct gtttctcggc gagtggctgc gtcgcgggca gctggtggcg
      961 gttgctctcg ccgcgatcgg cgtggtgaac gaagtcgtct ccgtggaggt catcccctgg
     1021 gcgggcctga ccctggcggt gagcttcggc ttctacgggc tggtccgtaa gcggctggga
     1081 gtggactcgg ccgtggggct gggcgtggag gccaccctgc ttctgcccgt ggcgctgggg
     1141 tacctggcgt atcagttcga ggcggggcag ggtgccctca accgtggcga aggcggcgag
     1201 atcgcgtggc tggctgccag cgggctcgtc acgaccttcc cactggtctg ctttgcggcg
     1261 gcggcgctaa agctgccgct gtcgaccctg ggcttcgttc agtatctggc gcccaccatc
     1321 acgttctttc tggccgtcct cgtgttcggc gaggcgtttc gctgggagca gagcgtcacg
     1381 ttcggctgca tctggttggc cgtggtgatc ttctccgccg agggcgtcta ccaccagcgc
     1441 aggctgcagc ggcgtcttgg ccgctgggcc tgaccagttc agcctgcgtc cagggagacg
     1501 gccctctcca cggcgcgttt gaggccggcc agcggacgaa tccaggcgtc gccgggctcg
     1561 atcggcgctg gcgggtcgag gcttgccgcc tcggcgaggg cgtagctttc gtagaagccg
     1621 agcagcagca cgtggtggat gcgcccatct ctctccaccc gggcgtgggg cagatacccg
     1681 aggccgtggg tatccacgaa cgcgctcagc tcggctgcgc tatccagggc gaccagctga
     1741 acggcgaagt agtcgtccgg gaggcccatc agaccatcgg cgtcaagctc gggttccgca
     1801 gcctttgggg gctccgggtc tgcggcggac ggctcttcga tcggaggctg ctcctggacc
     1861 gcgccgggct cgacatcccg cggaaccggc gattcggtgg ctgcgggctc tggcggttcg
     1921 gcttcgggac cctcgcccga ctcctccacg gggggggcgg gggtcgttgc agcggccagc
     1981 tgtggcgacc gagccagctc ttcatcctga atgcagtccc actcgtcgcc gccggtgcct
     2041 ggctgacaga accagttgcc ggtggattgc gggatctcca gcttgccgcc ctgcaggatc
     2101 ccgccacggc cgcaggccac gagcaacgcg aagagcagca tgatgggtag gcgcagcatg
     2161 gcgcgtactt tgcccaccgc cgcagttggt gtcaaagctg atctggcgag tcgagcaccg
     2221 tatgcccctg ggtcacgaag cggcccgaac gctgctgctg ggtgctcggg ccaggcgcgg
     2281 gcgcccgcgg gagctccacg ttgaatcgtc aggaacgatt ggtcatcata ccggcgccgc
     2341 catcggggcg agcgtgcccc gattgcgcat caatctgagg ttttgagctg ccaggcatga
     2401 ttgcaacgct ccccgagttc gatcgcgcgc ctcgggtgct gcgactgaac atcgccggcc
     2461 atcccgtgga ctgggtgagc tggcaggagg cggtgtgcct gtacgcccgg gacatcgtca
     2521 tctggaccct gggcgatccc atcctgcgaa ttcgcggcgg tcactcacgg ctggacagct
     2581 cccagtccag cgtcgaaatt cacgccatca tcgcctgcga cggccgcgta acgcggccgt
     2641 ccaggagcgt cccgcccctg actaacgaag cgctgttccg tcgggacctg aacgtctgcc
     2701 tctactgcgg cgggacgttt gccgacggcg ctctcacgcg cgatcacgtg gtgcccactt
     2761 cccgtggcgg acgggatgtc tgggacaact gcgtggcggc ctgcaggcgc tgcaatcatc
     2821 acaagggcag ccggctggcc gaggagtgcg ggatggaact gctggcgctg ccctacgttc
     2881 cgaactttgc cgagtacctg gccctgatca acagcggccg tatcctcggc gaccagatgg
     2941 actttctgcg aaagagcttc ggctcgaaca gccggctgct gcaataggcg tcggggcgtc
     3001 cggctagttc tgccgcgcct gccgccgcgc ctgccgccgc gcatgttcgg ccagcacggc
     3061 ggcaacgcag gcggtaatgc gcttgcccgt ggcgatttct agaaactcgt tcggcccgtg
     3121 ggcgtttgag cgtggcccga gaacgccggt cactacgaac tgcacgcccg ggaagctgtc
     3181 gcccagcatc ttcatgaacg ggatggtgcc gcccatgccc atgtacatgg ccggcttgcc
     3241 gaagtactgc tgggatgcgc gcatgagcgc gctttccagc cacggcgcga ccggcggcgc
     3301 gcaccatcca gtttgcggag tctcgagctc gaagcggacg tgggcgccgt tgggcgggct
     3361 ccgctccagt tccgccttga tggtccgcgc ggcctcgtct gcatccaggg ttggcggcag
     3421 ccggaagacc agcttggcgc tggtgcccgg tctgagggtg ttgcccgcgt tccccacgtc
     3481 cggagcgccg gcaaggccca cggtggcgag gctaggctcc catgcattgg ccagcagcag
     3541 gtcctgcagg tcgtcgccgt cgggagtgac gccgtccagc caggggaagc ggtcgatggt
     3601 cgtggcaccg agcacctggg cgacgtctgc gagctgacgg cgcgcatcgt cggggatggg
     3661 cacatacagg caatcgtgca gggaaccggt gtcggcatcc tccacccgat cgtagagcgc
     3721 gcgcaggatc cggaagctcg agggaacgat gccgccggcg gctccggagt gctgcccctc
     3781 gctgagcacc ttcacggtca gcacgccggg gagcatgccg cgcagcgagg tggtggtcca
     3841 cagctgctcg tagttgccgc actcggcgtc gaggcagatg acgagatcgg gctggccgat
     3901 tcgctcctcg agcagttcca cgtaggcggg cagatcgaag ctgccgcttt cctcgcagcc
     3961 ttcgatgagc agcacgcatc ggccgtgggg aatgccttcc gcctgcaggg cggcaatggc
     4021 ggccagcgag ccgaacaggg cgtagccgtc gtcggcgccg ccgcgcccgt acagcttgcc
     4081 gtctctgagc accggctccc aagggccgag gccgggctcc cagccgtcaa actccggctg
     4141 cttgtcgtag tgaccgtaca gcaggacggt cccggcgtcg ggcgccgtgg ccggcacctc
     4201 gcagaacaac accggcgtgc ggccggggat cgtgacgatt tcggtggtga ggccgccgat
     4261 gtcgggcacg gagcaccacc ctcgcaggag ctcgacggcg gcctgcatgt agccgtgggc
     4321 ttcccagtcg gcgtcgaaag ccggcgactt gttgggaata cgaacgtagt cgctcagcgt
     4381 cgggatgatg cttcgatccc acagctcgtt cacgaacggg gcgatggcgt cgctcatagg
     4441 tctatggcca cttgcaggta cacggcacgt cctggagatg cataacctag ttggtcctga
     4501 tagctctcgt ccagaaggtt ctcgccgcga agcgcgagct tcagatgccg gccgaggcga
     4561 taagccagat gggcgcgcag cagcacgtag tcgcccagcc gaacccgctc ggcggggaag
     4621 ccgctgaagt cgagatcctc ccggttgccc acgtagtagc ccgacagctc ggccgagaac
     4681 ggcccacgtt cgtggctcac ggtggcgtaa gcctgccact gcggcctgcg aatctctctc
     4741 gctgcgtccg gctcggcagc atccagccag ctcgcgccca tgctcaagac gcccgtgggt
     4801 atcgccagcg tggtctctac ctctacgccg tcgcgtttgc tggtgcccgg ctcgttgatg
     4861 gaggtgaacg cgccggcggc gccgtcgaag tagaagccgt ttatctcgtc ttccaggcga
     4921 tcgcggaagg cggtggcgcg cagggtccat tgccggtgcc cgatcaccgc ccccgccgac
     4981 agatgcctgt tggtctcggg gtccagatcc ggattgccga gaaacgtgtc cggggtgaac
     5041 ccgaagcgtt ccacgaagct cgggttcttg atgccggttc cccagttggc ccagagttcc
     5101 actgcccccg tcaggcgata acgggcagcc gcgcggtagc tctccttgtt ctcgaagtcg
     5161 gagttgtcgt cggtgcgtgc ggacagcgcc agggagagat cgtccagggg cgcccaaagg
     5221 tactccaggg cgaggctgtt cgttcgcacg gactgattct gattcggatc accgaagatc
     5281 gagggcgcgc cgcgctgctc gaatcgctcc ttttcgtgct ccagcatcag cgtgaccgcg
     5341 tgacgcgagc tcgcccgcag ccgggtgagg gaagtggctt tgaatcgggc cgcggccgtt
     5401 ctgctttccc gggcaccgtc ggcgaagctg cgattgtcgg tatcgaaata ggagaggcga
     5461 atctgctgct cgaaccgctc gtcgccgtgc caggtcgccg cgacgctgcc gaggtactcg
     5521 ccgtgcttcg agacgcgatc cccgtcgcgc ggctgaaacg caggaaacgg gatgggatcg
     5581 aagtccgcgt cgctcttcag gtgatgaacg agcgcctcga tggtccagga ttcgctgcgg
     5641 tagccgccgc tcaagcggta gctgctctgg tcgaagccgt cgtcctcctc gccggtggtg
     5701 gcgatgttcg ttccgtcggt tctgaattcg ctgagcgcga ggttgtagtg atacgagccc
     5761 gtcgtgtccg cgagctgcag cgccgcctgg cgcgtgtcga acgacccgcc ttccagggac
     5821 aacctgcgcc ggcgctcggt gggcttcgtg atgaagttca gcacgccggc caccgcgtcg
     5881 ctcccccaga tggcgctgtg ggcaccggcc agcagctcga cgcgcgtcgt gcccgtgagc
     5941 gagaggttgg cgaagttgaa gctcgcatcg gtggcagggt ctgccagctc gacgccgtcc
     6001 atgaggacca tgagatggtt ggcttcggat cctcgaacgc gaacctgggt cacggagccc
     6061 aggctgccgg cctggctgat ggcgagactc ggcaggctgc gcaaggcgtc ggcgcccacg
     6121 gcgggcccgg tcccggtcgt gcgagtcagg acggggagca ccggccggtc cgtgcgtatt
     6181 cgatgcgcgg ttacggtgat ggtctccagc tcggggtcgg gctcggcaaa ggagaggctc
     6241 ggggcgagca gggccagcac tgtggccagg accatgggca tgacggcaga ctccggcggc
     6301 ctgcctcacc ccgggcaggg ttgctgatac cgggatcggg ccggtctccg gactcgcgag
     6361 catcaatggc gtgcgccgcc ttcccaacgt ttcagcgtca gtggctgagc ctgctcagtg
     6421 gcggctccgg cgcggccgtc tcgattaccg ttgcgggggc agtgtcggat ttccaccgac
     6481 ttcccgttca cccgatccca ccgcgcggca accgcgcagc ggcgccagta tagctactcg
     6541 aaccctttga tgcagtcgta tccgtagagc cagtccatgc gctgcatggc gatctccggc
     6601 aggaagcggc tggtcagcga cagtcgcaga ttgcggcgcc aggctttccc gccggggtcc
     6661 gtggtcagct ctgccaggtg atgccgctgt ccctggcgaa agcggccgag gcgcaccttc
     6721 cggtagcgct cgtagtcggg gaaccccttg gccggctcgt cctcccagcg ctcgagcatg
     6781 cgcgacagca cccaggcgtc ttccatggca agcgacgcgc cctgctgcag gtgcggggtg
     6841 acggggtggc aggcgtcgcc caggagcgcg acccgccggt cgtaccactg ggcgatgggt
     6901 tcccgttcca ccggggcaga ccgggtgacg ccggttgccg agtccacgag acggaagacg
     6961 tcctcatgcc agttccggaa ggcgccggcc agcttcgccc ctgctcccgg gtttcgggca
     7021 aaggcaagga aacgcgcggc gcagccgtct gcagacggcc actgggtgag gtaggcgcgg
     7081 ggtccgagcc aggtggtgac cgcattggcg cgcagaccga ttggcaggtc ggtgcccggg
     7141 caggtgcccg tccagaccac gtcctgggtc gtctgcgtcg tctcggcgta tcccatgccc
     7201 gcgcgaacgg ctgaggcctc gccatcgcag ccgatgacga cgtcgtgaca gatctcctgg
     7261 gagccgaacg tcagcgttac gcttcgcgca tcctgagcga tgcgggcaca gggttggccc
     7321 gtgcgcacgg cgacgccgtg atgcgcgcat cgcgtcgcca gtgcttccag cagcgcctgg
     7381 cgggcgacga cgacctccgg cgctccgtac cgggcctcca tgaacgcgcc gaggggtcgg
     7441 tgcgccagca cgaagccgct gcgccagctt ctctgcacgt aggcatccgg cttgtgggca
     7501 cgagatagaa acgggcccag gtgcagcgcg cgcagcacgc gggtgccgtt gggcgacagg
     7561 accaggtcgg atggctcagg ggagcccggc tcgccccggt cgtagacggt gacgtcgcga
     7621 aatccagctt cgccgagggc ggctgcaagg gtcaggccgc cgatgccggc acccacgacc
     7681 cccaccgatg gcgattcagc cgtcccgcag cttccagcgt gtgccgtcgc gggtgtcctc
     7741 cagttcgatg ccgagatccg tcagctcctc gcgaatctcg tccgcgcggg cgaagtcttt
     7801 cgcctttcgg gccgcgttgc gttcctcgat caagcgatcg atctcggcgg cgtcaatgtc
     7861 ccgcccggcc gtgaagtgcg cttttgcgga ctcggtgaga atgcccagca gccagccgcc
     7921 ggccagcagc cgtcgtgcca gctcgacctt ctcggaagcg tccgacgtgc gatgaatggc
     7981 gcttgccagc tgatgcatgg cggccagggc ctggggcgtg ttcaggtcgt cggcaagcgc
     8041 tgccaccacg acggtcgggt actcttcggc gggcaggtcg cggtagtcgc tggatgtctc
     8101 gtcctccacg ccggcatcac gcagcgcctg atacagacgc tccaggctgc tacccgcctg
     8161 gtcgaggaga tcctccgacc aggccagctg ggaacggtac tgacccgaaa gcagcgcgta
     8221 gcgcaggact tcccctggaa agcgatccag aaggtcgcgg atggtgacaa tgttccccac
     8281 ggacttggac atcttctcct ggcccatggt gagcatgccg ttgtgcagcc agtagcggac
     8341 gtactcggga tggtcgttcg cgcagcggct ctgagcagct tcgttctcgt ggtgaggaaa
     8401 cgccagatcc gagccgccgc cgtggatgtc gatggtctgg ccgagatgct tgctgatcat
     8461 cgccgagcac tcgatgtgcc agccgggccg cccccgtccc caggggctgt cccagcccgg
     8521 aagatccgtc gtggaaggct tccacagcac gaaatccttg gggtcctgct tgtacggcgc
     8581 tacctctacc cgcgcgccgt cgagcatgtc ctcgaggctg cggtgggaaa ggctgccgta
     8641 ttcgggatcg gagggaacgt ggaagagaac gtgcccctga gcctcgtagg cgttgccctt
     8701 ggcgattagg gtctcgatca tcgcgatgat ctctgcgatg tggtgtgttg ccctcggttc
     8761 caccgtgggc tcgagcacgc ccagcgcggc cacgtcttcc cggtaggcgc tgctgaaacg
     8821 gtcggccagg gcctggatgg gctcgtcgtt gtcccgggcg gcggcgttga tcttgtcgtc
     8881 gatgtccgtg atgttgcgcg cgtagaccac ctcggggtgc gcggcgcgga gcagtcggta
     8941 gagcacgtcg aacaccacgg ccgggcgccc gttgccgatg tgcaccaggc cgtagacggt
     9001 aggcccgcag acgtacatcg ttatgcgctg gggatcgacg ggatcgaagg cttccttgcg
     9061 gccggatttc gtgttgtgga gtcggatgtc cataagcggc tcgccgtctg ctcagaccgg
     9121 ttcggacgac acgcgcgcgg tgccgtcgct gacgctaagg taaaagcacg agggccggtt
     9181 cgtgtggcag gccggccccg tctggtccac gatgagcagg atggcgtcgc catcgcagtc
     9241 gaagcgcatc tccttgagcg cctgcagatg tccggaactc tcgcccttgc gccagaaggc
     9301 cccgcggctg cgagaccagt agcaggcgta gccgtctttc agggtgcgtt cgaccgcctc
     9361 gcggttcatc caggcgagca tgagaacgtc cctgctgtcg gcgtcctggg cgatggcggg
     9421 caccaggccg tccgagtcga acttgaccgc gtccagcagc gcgtcgaggg cgacctcgtg
     9481 gccgagctcc gcgtcctcga ggtattcgaa catctcagcc tccgaatcgt tcctgcaggt
     9541 agccgaaaac ggccctgatc gccatggctt ccgcaccctc gggtcggccc gggcgggggc
     9601 gtgtgttgaa ggccatgatg tcgaagtgca cccacggcgc cgagcccacg aacttctcca
     9661 ggaagagggc ggccgttatg gatccggcga acggcgagct tgccgagttg acggtgtcgg
     9721 ccaccttgct gtcgagcagg taggcgtagg gtttgtgcag cggcagccgc cacacagggt
     9781 cgtcccagcg gtcgccgtgg gcgctcagcc cggcggcgac ctcgtcatcg ttgcagaaca
     9841 tcgccgcgat ctctgcgccc acggccgatc gcgcggatcc ggtcagcgtg gcgaaatcga
     9901 ccatgagggc gggttcgtcc tcggcaccca gcgccaggca gtcacacatg accatgcgcc
     9961 cttcggcgtc ggtattgtcg atttccaccg agatgccctt gtaggtcggc acgacgtcgc
    10021 caggccggta agcgttcgcg ccgacggcgt tctccactgc cgcaatgagc acgcgcagac
    10081 gaacgggcag attgctcgcc atgatgagct gggcgaggcc gagagcgttg gctgccccgc
    10141 ccatgtcctt tttcatgagc cgcatactgg cggccggctt gatgtcgagc ccgccgctgt
    10201 cgaagcacac ccccttgccc accagcacga tcagcggatg gtccgggtct ccccaggtga
    10261 tgtcgatcag gcgcggcgcg tcggccgccg cccgcccaac ggcgtgaatc gcgtcgaagc
    10321 cgccctccag cagctgctcg cccaccagca cccggcagtt tgcctggttg gcgtccgcca
    10381 cctggatggc ctcgtctgcg agatggctcg gcagcatgtc gcctgcgggg atgtttatga
    10441 gatcgcgcac cagggtagtg gccgcgtcca tgttcgcaac gaggtccgcg tcgttgcgtg
    10501 caggaatcag gagctgggcg ggagctctgt cgcgctcccg gtagcggacg aactgatagg
    10561 aaccgagcgc ccagccgagc agctgcaggt cggagacctc ggttttcagc tcgtacacgc
    10621 cttcgggtat gcgggtggga aggccgccca ggctggccag gtcgtccccg ccgtcccatc
    10681 ctgccaccag ctcggccggg tggccgtcgg ctccgggcag ccaggcgaac tcgccgctct
    10741 tcgccgcgaa ctgagcacgc cggatccagc tccgttcggc ttccgaaagc tcgctggcaa
    10801 acgcctcgaa gtccgaggtt ctgatcagcc tgagcggcgt tgcggcggcg tcggatgaaa
    10861 cgatgagcgc ttctgccatt ggggctccgg ttggttgaag tccgagatgg tctatgatgg
    10921 cctcccggtt cgcaagcggt ctgagccgtg ctcacggaag tcaccacgca tcacctcgag
    10981 ctcaccgatc cggatcagtt caagccggcg gccaccggtc cggcctacgc gctcaagcag
    11041 gtccagcgtc ccaccgcgga gctgaaccgc tttctctact cgacggtcgg ggcggcgtgg
    11101 ttctggtacg agcgcttgag ctggtcccgg gcccgctggc tcgagtttct cgaccggccg
    11161 gaggtcgtca cttgggtcgc ctatgtggat ggcagccccg ccggctattt cgagctggag
    11221 gatcagccgg gcgggtcggt ggagatctgc tatttcggcc tcctgccggc ctacatcggt
    11281 cagggcatgg gcggcgctct gctgacggat gccatccgcg ccgcctgggc caggggcgcg
    11341 tcccgcgtct ggcttcacac ctgctcgctg gatcaccctt ttgcgctcgc caactatctg
    11401 gcgcggggct tttccctgtt cgacatcgtc acgaccacga aaaatctgcc cgatcccatg
    11461 ccggagcccc ggcccgggga tgccgaggcg ggccggtcgt cagattgatt tgaccgggat
    11521 ccgcccgagc ccgaggttgt tgccttcgag tatgcggtcg gcccggtagc tcgaccttac
    11581 cagcgggccc gacgcgactt ccgtgaagcc acgctccaag ccccattccc gataccgttc
    11641 gaactcgtcc gggtgcaccc agcgcgccac tgccagatgg ttgcgggtgg gacgcaggta
    11701 ctggcccagg gtgaggatgt cgacgccgtg ctcgcgcaga tcgtccatgg cctggcaaat
    11761 ctcgtcatcc tgctctccca ggccgagcat gaggctcgat ttggtgagca agtcccgacg
    11821 gacctgcttg gcctcggcga gaacgcccag cgtctgatcg tatcccgccc ggggatcgcg
    11881 aacgtcgccg gtcaggcgct ccacggtttc cagattctgg gcgaagacgt ccagccccga
    11941 gcccaccacg acggcaacgg cctcggcgga tccgctgaaa tccggcgtga gcgcctcgac
    12001 ggcggtctcg gggttgaccg ccttgatggc ctggatgcac ttcgcgtagt ggccggcgcc
    12061 gccgtccgcc aggtcgtccc ggtcgacgga ggtcagcacg acgtagcgca gccccatcag
    12121 acgcacggac tccgctgcgt ttgcgggctc gtccggatcg agacgccctc cgggattgcc
    12181 cgtgtcaacc gcgcagaacc ggcaggcccg ggtgcacacg gagcccatca gcatgatggt
    12241 cgccgtgccg ttgttccagc actcaccgat gttcgggcag tgagattcct cgcacacggt
    12301 ggccagacgg tgggtggcca ccgtcctgcg cactgcctgg tacttttcgc cgccgccgag
    12361 ccggacgcgc agccacgggg gcttgcggcc ggtagccggc tctgcctgcc cgttcgcctt
    12421 catgccgtcc ttgatggcgg tgaaaccttc gggcgtgcgg aatttcttgc cgctgacgac
    12481 tggtttgctc atggcccgtc attgtaccca taatgccgcg tcgttgaggt gcggtaacca
    12541 atgcaggaac gtccggcagt cacccggcgg gtgcgatggc tatacggggc cggagccgcc
    12601 gcctacggcg tgaaggaaaa cggcttctcc tatttcctgc tcttctacta caaccaggtg
    12661 ctcgatcttc cgggcgccta tgccggcacg gcgatcctga tcgccatgat cttcgacgcc
    12721 atctcggacc ctctggtggg cgtctggtcg gacaatacca ggtcgcgctg gggacgccga
    12781 caccccttca tgtacagcgc cgccctgccc gtggcggtga tctacttctt tctctggaac
    12841 ccgccggagc taacggagtt tcagctcttt gtctatctca ccgtagggtg cgtgctcgtg
    12901 cgtttctttg tcacgctgta cgagataccg tccagcgcca tcgtcgcaga gctcacggac
    12961 gactacgacg aacggacccg gttgctcagc taccgctaca tgatgggctg gttcggcggt
    13021 ctcgggatgg cggtgctgaa ctggggtttg ttcatggtgg tctggggaga ggcgggcgaa
    13081 acgacgttcc gcgtctacgg agcggtgggt gcggtgatca tgttcgtggc catcgtggga
    13141 tcctccctcg gcctgcaccg ctacataccc tacctgcacc cgccgccgga aaggacctcc
    13201 taccgggtcc ggcagcttac cggcgatctc atcgagacca tcagcaacag gaacttcgcc
    13261 gcgctgttct tcgccggcct cttcgccgcg gtgggcgccg gcgtggcgac gaatttcgac
    13321 acctacatca tcacccactt ctgggagttc gaggccgagc agtggcgctg ggtgatcatc
    13381 tcgttgttcg tctcggcact cattcccatg tatctggcgc cgctggttac ccggcgctgg
    13441 gacaagaagc gaagcgcgct gggtatctac gctttccaga tcgtctttgc cgccctgcct
    13501 atcctgttgc gcctggccgg ctggtttccg gataacgaca gcccctggac ctacccgctg
    13561 atctgggctc acgccgtcgt caacgtggtc gtgatcgtga tgttcggcgt catccagagc
    13621 tcgatgctgg cggacgtggt ggagcacagc caggcgtcca ccggccggcg tgaggagggg
    13681 ctgttcttcg ccgcgcgatc gtttgcccag aaggcgacct cgggggtcgg cgcgttcatc
    13741 gccgggctgg cgctggactg gatcgcgttc ccgaggggcg cgacgccggg gacggtgccg
    13801 gacgccgtca tatgggatct tggtttcatt tacggccccg cgctcatgat tttctacgtg
    13861 ctggcgttgg tttccatcgg gttctataga atctcgcggc agggacacga tcagcgggtg
    13921 ggcgcgctgc gcgcgggcgc cacggccccg tcgggaagca tggaggaatc gcgatgaacg
    13981 accgacccgt gatcgtcacc gggggcgccg gcggcatcgg cagcgccatc gttgcccgtc
    14041 ttgttgccga cggccatccg gtggccgtgc tcgatctgca ccccgcgtct gctacccctt
    14101 cgggctccct gttttcctac ccgtgcgaca tcgccgatct cgacgcggtg gagcgggcgg
    14161 tcgccgagtt cgaggcggcg gtgggcgcgc cgttcgggct cgtcaacaat gccggctggg
    14221 accaggccct gccgtttctg gagacggggc cggatctgtg ggagaaggtg atcgacgtga
    14281 acctgcgcgg ccccgtgaac gtcacctacg cggtgctgcg caggctggcg ggcgcgggcc
    14341 gcggccgggt ggtcagcatc gcctcggatg ccggccgtgt gggctcgtcg ggagaggccg
    14401 tctactcagc gtgcaaaggc ggcatcatcg ccttcatgaa gacgctggct cgtgagcacg
    14461 cgcgtcaggg catcacgttc aactccgtct gccctggccc cacggacacg cctctgctcg
    14521 attccttcga cgagtcgggt cggctggccc aggcgctcga gcgggccata cccatgcggc
    14581 ggctgggaag accggaggac tttccgggga tcgttgcctt cctgttgagt gacgaggcgt
    14641 cgttcatcac cggccagacc gtgagcgtct ctggcgggtt gtcgatgcag gggtgaccta
    14701 cccctcgtcc tgaaactgca gcgagtgcag ggtgtgatag agcccctcgc tggcgatcag
    14761 ctcctcatgg gtgccgagct cgaccaccct tcctccctga agaaccagca cccggtcggc
    14821 ttcctgaatg gtctgcaggc gatgggcgat gatgatcgaa gtccgcccgg cggtgagctt
    14881 tcgcagcgcg tcctggatga ggagctcggt ttcggtgtcg atgctcgcgg ttgcctcgtc
    14941 gagcacgagg atctcgggat ccgcggccag cacccgggcg aaggccagca gctgccgctg
    15001 cccctgggac aggttctgac cgcgctcgga gagctcggtg tcgtatccgc ggggcaggtc
    15061 gctgatgaag ccgtgggcgt tgacgcggcg cgcggcctcc tcggcgcgct cccgggtgat
    15121 ccggggatcg ccgagggcga tgttgtcgaa gacggtccct gagaagatgt ggaagtcctg
    15181 gagcaccacg ccgatgcgct ggcgcaggtc gcgcgtatgg atgcggttga gatcgatgcc
    15241 gtcgaggaaa atctggccgt cgtcgaattc gtagaacctt ccgagcaggc ggatcagcgt
    15301 gctcttgccg gaccccgtcg ggcccacgat ggcgagcttc tccccggccc cgatctcgaa
    15361 ggagacgtcg tggagaatct gaacgtccgg gtcgtaggcg aagttcaggt gcctgaaggt
    15421 gacctcgccc tgcagccggg gcggcagcgt gacgggatcg gccggctcgt gaatcttctc
    15481 gtcccagtcg agcgcctgaa agatccgctc gccgctcgcc atggcccgga agagaatgtt
    15541 cgtctgctcg ccgagaacca cgatgggatg gaagagcatg tcgatgaagc gggtgaacag
    15601 aatcacggcg cccagggtca tttcttcctg cactgcagcg ccgccggcgt accagatgat
    15661 caccccgagg gccaggtgcg ccatgctgtc gttgaaggcg ccgtagaggg tctcgaatcg
    15721 cgtcgttctg agctcgtggc cgcggttttc ttcgttgatc ttcgtgtaac ggtccaggtt
    15781 gtattgctgt cgctcggaga gctgcaccac ctgaaggccc gccaggtttt cctgcatgtt
    15841 ctgattcaga gccgacagcg acatgcgcac catccggaac aggcggcgcg tcgcccggcg
    15901 gaaatagaag gtcacgctgc ccatgacggg caacgcgaga agcagaagca gggttagctc
    15961 gacgtcgatg gccagcatga tggcgagcgc cacgaagaag ggcatgaact cgccgatgag
    16021 cgagccgaga ccgcgaagca gctcgtagag cgcttcgatg tcgttggtca cccgcgtcat
    16081 gacccgtcct acggcgactc ggtcgtagaa cgaggacggt ctggtctcga ggtgtcgaaa
    16141 cagatccagc cgcaggtccc tcagaccgtt gatgaccgac gagatcaggg tcatgcggtg
    16201 agcgtgaccg gcagccgccc aggcaatctg cagccccgca tacaggagac aggccgccat
    16261 gagagggtga agtgccagga tgtccgccag ccactcggtc agctccacca tgccgaggtc
    16321 cggtgccagg gaatccgtct cgccccggat caggtggtcg atcaccacca gcgagatgat
    16381 gatgggcatg agaacctgca gcgtggacgc cagcagcacc agcacggcgc tgattccgaa
    16441 ggtcacccgg tagggccgca accatttcag gaggcgtcgc aggagcgcaa ggttcagaac
    16501 cgcgccggag atctcggcgt cgaacatgga gtagttgcgc cgctcgggag cgccaggctg
    16561 gctggccgcc gtcacgtcgt cgccccgatc aggtcgtagt cggtatcaac agcgccctcg
    16621 cgctgaatcc gttcgagctc ggcgtactgt cctgccctgg ccagaagctc ttcgtgggta
    16681 ccggactcga cgatgcgccc ggcgtccatg accacgatgc ggtcggagtg gcgggcggtc
    16741 gaaacgcggt gggacaccaa aatggtggtc tggcggcgcc gcagccggcg cagctcccgg
    16801 agaatgtgct cttccgtctc ggtgtcgacg ctggacaagc agtcgtcgag gatcagcacg
    16861 ggcgccccgc gaatgaggcc gcgggccagg gtggctcgct gcttctggcc tccggagagc
    16921 gtcacgccgc gctcgccgac gatggtgtcg agctgctccg ggaactcctc gatggtttcc
    16981 ttgagatctg cggacgttgc cgcctcccag atcttctcca ccgcccggcc cgggtcgtcg
    17041 taggtcaggt tgtcgcccag gggctcgccg aacagaaaag catcctgggg caccatggca
    17101 acctgggacc tcagctgggc gagtgggtag tcgcggacgt cgtggccgtc gatgagaacc
    17161 gcgcctggcg gcgtgtccag cagccgcacg agctgcttga gcagcgtcgt cttgcccgat
    17221 ccgaccctgc ccatgatggt cacggactcg ccccgggcga cggtcagcga gacgtcatcg
    17281 agagccggac gaatgccctc gtcgtaagcg aaggtcaggt ggcgtatctc gacgtcgccc
    17341 cggatcgtcg ccggcgtcgt cccgcttgga tcgtcggcga tctctgggga cagggtgatg
    17401 acctcgaaca gccggtcgct ggccacggcc gcccgctgga agaggttgac gatgaagccg
    17461 gccacccgaa acggctggac gaccatgttc acgtacatga agaaggcaac gagctcgccc
    17521 accgtcatgg cgccgtccag gaccaggtag ccgccatacc ccaggatggt gacggagcag
    17581 atggcggcca gcgaaggcat ccaggccgcc atgagcgagt tgatctgaga ctggcggtag
    17641 aacgcgttgg cgtactgctg gttggtcctg ccgaaccggc ggatctcatt ctcttcctgg
    17701 accatggcct ggatggtgcg aatcccggag aggttttcct gggcgtgggc gcccaggtcg
    17761 gacaatcggt tctgaacgtc cctcgaggcc acgcccatct ggcgcgcgga ccagcttgcg
    17821 tagaagaaca ccaggggcag gggtggcagc agcagcagcg tgagtgacgg ggacagatag
    17881 agcatgaagc cgaagcccac cagcgtcgcg tagacgagga tgaccatcag gatcgtgccc
    17941 atggcgatga gtctctggat caggccgatg tcggctaccg cccgggtcat catgtcgccg
    18001 atggtgtagc gcgtgaagaa acgaacgccc tgttgctgca gggattcgta cagagcctgc
    18061 cgcagatcga acgccacgtg ctggccgacg cagcgcacgg agtagcgggc gtaggtgacc
    18121 acgacgtagc gcccgatcac cgctccggca attccgagca gtggggtcgt catgtccgcg
    18181 ttgccggcgg acatccgatc gatgccgatg gccacgaaga taggcaccat ggcttcgaag
    18241 aatcttgcga ttccgaacgc gccgacgccg gcccccagtc gcagccgata cggggcgagg
    18301 aacgatttca gacggatcag agagcgaatc atcggtgggg cgcagccgct gccgcgtcag
    18361 aaactggcgt tgtgcggcac tctggggaac gggatggcgt cacggatgtt gtccatcccc
    18421 gtcatgtaaa gcaccaggcg ttccagaccg agcccgaacc ccgcgtgggg tgcggtgccg
    18481 taccggcgaa ggtcccggta ccaccagaga tcctcgccga tgtcctgttc cgccatgcgg
    18541 gcgtcgagca cgtccaggcg ctcttcccgc tggctgccgc cgatgatctc gccgacgccg
    18601 gggacgagca cgtccatggc ggccaccgtc gcgtcgtcgt cgttcaggcg catgtagaac
    18661 gctttgatgg tcttggggta gttgaccacc accaccggcc cgcccacgtg cacctcggtg
    18721 agatagcgct cgtgctcgga ttgcaggtcg agtccccact gcaccgggta ctcgaagttc
    18781 tgacccgcct tctcgaggat gctgacggcg tccgtgtagt cgatccgttc gaacggtgtg
    18841 gcgatgacgt gttccaggcg gctgatggcc tgctcgtcga cccggtcggc gaagaaactc
    18901 atgtcgtcgg gacaggcgtc cagcaccctg cggcagacgg ctttcaggaa gtcctcggca
    18961 agctccgcgt tggcgttcag gtcggcgaag gcgatttccg gttcgatcat ccagaactcg
    19021 gccagatgtc ggctggtatt cgagttctcc gcgcggaagg tcgggccgaa cgtgtacacc
    19081 ttgctcagag caaggcagta gctttccagg ttcagttgcc ccgagacggt cagaaaggtc
    19141 tcgcgaccga agaagtcgtc gtcgaagggg ctgtccccgc gggtcagtgc ccggttggcg
    19201 atgtccagcg tggagacgcg aaacagctgc ccggcgccct cgcaatcgct ggccgtgatg
    19261 atcggcgtgt tgacccagac gaaaccgttg tcgttgaaat agccgtgcac cgcctgggcg
    19321 atggcgttac gcacgcgagc gatggcgccg aaggtgttcg tgcggggacg cagatgggcg
    19381 acttctctga ggtattcgaa ggtgtggcgt ttttttgcga tcgggtaagt ttcagggtcg
    19441 tccacccatc ccaccaccac gacctcggtg gcctgaatct cgcggtcctg gccgcggcct
    19501 tgagattcca ccacggttcc cctgatccgc acggagcagc ctgtgccgag atgctcgaca
    19561 tcgtgctcgt agttggcgag ctgcgagtcg gccaccgcct gaatggtgtc gaagcaggag
    19621 ccgtcgctga cgtgcacgaa ggagaagccg cctttcgacg ttcgcctcga tctgacccaa
    19681 ccttcgatgg tgacctctgc tccgacggga aggcggtggg cgaggatgtc tttgatggtg
    19741 tcggtcatcg gcgcggctgc tcaggcagga atgagggatt gtggaatgat aatggaattg
    19801 gcccggcctt ggataattcc ccgcgggtcg tggtcataat gagacatgcg caacctcatc
    19861 atcgtcatcg ccctcgtgct ggttctgctc ggcggcctct ggctggcttc ggatcgcagt
    19921 gccggtgatc gggaagtccc gctcccgggc gccgagccgg tgccggttcc cggaccgcgg
    19981 cccgctgaac ccgcgcggcg accgcagccg atgcccgtcg ctgagccggc tatcgtgctg
    20041 cccgacctcg acgcgagcga tccgttcctg cgggaacggc tggctggggt cgagttgccc
    20101 cagaactgga tcgaccgtga tgatctcgtc cgccgcctgg cggtggtgat cgaaaacgcg
    20161 gctcgcggag agtatccaag gcgtcagctg ggttttcttg cgccgtccgg ccgtttccgc
    20221 gtggtggaaa gacagggcag gctcttcgtg gaccccgaca actaccgacg cttcgacggg
    20281 tatctcgacg tgctcgaagg cctggacccc cggacggccg ccgagctgct gtctctcatc
    20341 gagcctctgc tggccgtcgc catgggcgag ctcggcagcg acgggtcggt ttccgatcag
    20401 atcgatgctg ccgttgcgga ggtgatggcg gtgcccgacg ttgtcggcga ggtggagctg
    20461 gctcagccga aggtgctcta cgagtacgcg gatccggcgc tggaagcgct gtcgccgctg
    20521 cagaagcagg tgcttcgaat gggtccggaa aatgcggcgc ggctcaagcg ctacgttgcc
    20581 gagatggccg tcgcgttgca gcctgacccc tccgacggct aggcgggcgc ccttcggcgc
    20641 aagccgttca gccgatgtcc agggtgcggg tattgtggaa cttgtccagg ctgacggtgg
    20701 acttgaaggg gctgcgttcc tcgatgtact cgatgtcctc gcggacgtgc tcgcgctcct
    20761 cgtccagata gcgggcaacg gcattacgaa agcccgcgtc ggctatccag tgggcgctgt
    20821 aggtagggca gggtagatac ccccgggcga ccttgtgcgg gccctgggcg ccggcttcca
    20881 cccttttcag tccccgctcg atagcgaagt cgatggcctg atagtagcag gcttcgaagt
    20941 gcagaaaggg gtggtgctcg atgcaacccc agttgcgacc gaagagggct tccccgccga
    21001 tgaagttgag cgcgcccgcg atgtagcgcc cgtcccggct gcacatgatc agcagcacgt
    21061 cctcggccat ggtctcggtg acgaggctga aaaactgccg agtgaggtat ggggaccccc
    21121 attttcggga tcccgtgtcc atgtagaagc ggtagaaggc gtcccagtgc gattcctgga
    21181 tgtcgctccc cgtgagccat tcgatcctga tgccggtggc cagggcgtcc cgccgctcgc
    21241 ggcggaggtt cttgcgcttc ttcgaggaga gatcgccgag aaactcctcg aagctcccgt
    21301 agcctgcgtt gtgccagtgg aactgctgat ccatgcgccg aagaaagccg agctcgccgg
    21361 cgatctgcca ctgggcctcc ggcaggaagg tgacgtgcag ggacgagact ttgatctctg
    21421 ccgctacctg ggcacacgcc cccagcagcg cccgctcgat ctccggccgc gagacctcat
    21481 cttggccggc ggcaagcacg cgccgaccgg ttgccggcgt gaacggaacg ctgacctgca
    21541 gcttggggta gtagtcgccg ccggcccgat gccaggcgtc agcccaggcg tagtcgaaca
    21601 cgtactcgcc ctgggagtgg ttcttcacgt acatgggcac cacgcccaga atacggtcgg
    21661 cttcttcaag gaccaggtga aagggttgcc agcccgtcct ggccgcggcg ctgccgctgt
    21721 gctcgagggc agccagaaac tcgtggcgca ggaacgggtc gtggggaatc tcgccgcctg
    21781 gcatcgcgca ccggttccag tcttcggccg gtatctcgtc gatgctgtgc agaattctcg
    21841 cggtgcggga agtcaatcgg ttctccttcg ttccgagttt aaccgctcgc cgcaaggtgc
    21901 gctgccctgg caggcgaacg cttcagaaaa taccggcctc gaggccggcg gccagggtca
    21961 ggccgagctc ttcgcagcgg tcgatgtggt cgctggtgat ctctccccgg gcgatgacgg
    22021 gctcctggac ttccttgaac ggatatccgt tggcgatgcg tcggatggcg cgcagcgccc
    22081 cgctgccgtc gtttccggcg ctgatgaaga tcgcgtaggg cagggaaaga atcttcccct
    22141 ccacggggta gaacgtccgg tcgagaaagt ccttcatgcc gccggacatg tagccgaagt
    22201 tctcgggcgt gccgagcagg aggccgtcgg cccagaggag atcgtctgcc cccgcgttct
    22261 gcgccgtcag caggcggacg tccacgtcgc tcacgagctc gttggtggca cctctgtgga
    22321 cggccgccgc cagctggccg gtgcggccgg tctgcgtgtg gtagacgatg agaagatgct
    22381 tcatctgctg gcgggaggtg ggcgaggctg gtgggtcgtc gagtatactg acgccttttc
    22441 agaagttcga tcgtgaccca gctcgacgac attactcagg cccggcagcg catctgcggc
    22501 cagattgagc tgacggcctg cagccgctca gcgacgctgt cggcaatgac gggcgcggac
    22561 gtccatctca agttcgagaa cctgcagttc acggcgtcgt tcaaggagcg cggagcgctc
    22621 aaccggttgc tccagctgac gggcgagcag cagcgcttcg gcgtggtggc catgtcggcg
    22681 ggcaatcacg cccaggccct ggcctatcac ggcaagcggc tgggcgtacc cacctgcata
    22741 gtcatgcccc ggttcactcc gaacgcgaag gtcgagcaga cgcgcgtcca cggcgcggaa
    22801 gtgctgctgc acggcgccga gttcgacgag acgctggcgt tcaccgaagc cctggccgag
    22861 gagcgcggcc tcacactcat tcaccccttc gacgatccgg cggtcattgc tggccagggc
    22921 accctggggc tggagctcgt ggagcaggtc ccgggactcg aggcggtgct ggttccggtg
    22981 gggggcgggg gcctgatcgc cggggtggcg acggcggtga aatcggtgaa gccggaggtg
    23041 gagttgctcg gcgtgcaggt ggaacgcttc gcttcggtct atgcagcctt tcacggccgc
    23101 gaagtgccgc cggggccacc cgcgacggtt gctgaaggca tcgcggtgcg cgccccggga
    23161 aagaacaccc tgccgctgat caggcggcac gtagacgatg tcgtcctcgt ctccgaggag
    23221 caggtggagc agagcatatt caagctgctg gagatcgaga aaaccgtggt cgagggggcc
    23281 ggcgccacca gcctggccgc tctgctggct gagcccgggc gttttgccgg ccgccgggtg
    23341 gcctgcgtgc tcagcggcgg caacatcgac atgatgatgc tgtcgtcggt cgttcagcgc
    23401 ggtctggttc gcagccggcg gctcgttcgg ctgagcgtgg agattcccga cgtgccgggc
    23461 gcgctcagcg agctcacgcg ggtgctgggc gccctggaca gcaacatcgt cgacattgcc
    23521 catcagcgag ccttcggcgg ctcatcggtg cgggccacgc gggtagacct ggtgctgcag
    23581 atgcgcggtg aggagcaggt ggacgaggtc gtccaggagc tccaggcgcg gggctacgaa
    23641 tgcgcccgcg gcgggtgaat cccggcggcg cgccatcgtc tgtcgggtta tattgacggg
    23701 gcaatcggaa ccagagaact ttcggggagg cgtcatgcgc gtaaacgaac ccaggatacc
    23761 tgcgctcgag caatccgagt ggagcgaggc tgctcaggag atcatggggc cgatggttgc
    23821 ccaggatcgg gtcttcaaca tcttcaggac gctttccaat cacccggacc tgatgcgccg
    23881 ctggctggtg ttcgcgaatc acatcctggg caagtccacc ttgcccgttc gtgagcgcga
    23941 gctggtgatc ctcaggatcg gctatctgtg ccagtcgggg tacgagtggg ggcagcacgt
    24001 ccggatcgcg caggaagcgg ggatgacggc gcaagagatc cgctctgcga aaacgggccc
    24061 ggacactccc ggcctttccg atctggaccg cctgctgttg aaggccaccg atgagctcca
    24121 cgcggacgct cacgtgagcg acgagacctg gaaggcgctc accgggcatc tgaacaccca
    24181 gcagctcatg gatctggtct tcaccgtggg gcagtacaac atggtttcca tggttctcaa
    24241 cagcttcggg gtacagctgg acgaggggct gcctggctgg gacatttagg gtgggcttgg
    24301 ggagttgacg gctattctgt aaaaaaggct gctcaaaaac ccgaaacccc tatcttgccg
    24361 caaacctaaa gggacccgca cggtctaact tatgtagatg gttagccgtt ttgtcaccat
    24421 cggtaacaaa gtggttgact ggataagggc tattttggac tattcaggtg gctgtagcac
    24481 ataaacacaa aggctccgga cgtccccgcc gtcaacgggc ggaaggacga gaatgcctgg
    24541 gcaagtgcct gaaaagcgct gctcgacggg cagacaacca gacagagagc cgtgccgtgc
    24601 tcacaggtgt gacaatgaca tgaaagggag tgtcgcaggg catgctgtat cttaacgggg
    24661 caatcggttg catcttcatc gtcatcgcgc tgctccacgc tccgcatccg gacaccttta
    24721 gctgggcccc ttacctcggc gccgctctgc tggctctgat ctcctgtcgc cgtgaagtca
    24781 gtatgggggt ggcccgggcg ctcgcggtgc tgaccatggt ggccatgttc tacttctttg
    24841 ccgctttctt cgtgcacgtg cccaggttgg cagccgactg gtatatgcat caggaaggct
    24901 ggagcgcggt ctgcgaaatc ctcgctgcgt ttgcgatgat cccgattctt tccgatttca
    24961 gctgccgtct gaaagccgaa tgccgtgagg cccgcgcggc tcgccgtgcg ttcttctcgg
    25021 tgccgagcaa catccagccc catcgctagc aacgcgtaat catgcagttc cggcctcgcc
    25081 gatccgtgct ttacatgccc ggcgccaacg ctcgcgccat ggaaaaggcg cgggaacttc
    25141 cctgtgatgc catcattttc gatctcgaag atgcggtggc cgtggatgcc aaggtcgctg
    25201 ctcgggatca ggtggcccag gcggtgagtg ccggcggcta cgggtatcgg gaactcatcg
    25261 tgagagtaaa cggcctggat accgaatggg gccgtgatga cgttgcagcc gcagcccgcc
    25321 tgccggtgga tgcgctgctc tttcccaagg tcgagtcgcc ggagcaggtt gcgaccatcg
    25381 tagctgcggt ggatgccgct ggcggtaaag cgctgccggt gtggctcatg atcgaaaccc
    25441 cggccggcgt cctggacgct ccggcggtgg cctgtgggtc accgagggtt cgttgtcttg
    25501 tcatggggac cagtgacctg gtcaaggaac tgcgagggcg gcacacgcga acccggagca
    25561 atctgaactt cgctctgcag cgttgcgtgc tctcggcccg atcggcaggg gtcgacatcc
    25621 tcgacggggt gcatctggat ttccgcaatg cggaaacgtt tcgtgatgcc tgcgagcagg
    25681 gccgcgacat gggatttgat ggcaagacgc tgattcaccc gacgcagatc gaggccgcca
    25741 acgaggcctt cggctactcg gcggcagacg tggatcatgc ccgggcggtc attgcggcat
    25801 gggaatccgc gcaaagcgaa ggcaaagggg ttgccgtgct cgacgggcag ctcgtcgaga
    25861 acctgcatgc ggcagaggcg cagcgggtgc tcgcgttcgc ggccgcggtc ggcgatcgct
    25921 gacgggccta tccgatcagc agcagacgtc cgccgctatt cgcagagggc gcggaccttg
    25981 ggcatgatgt ccgtgaggta agcgtccagc gcctcctcgt tccagggcgc gcgcaaagcg
    26041 atgttgatct cgtccgcgcc ggcattcacg tactccatga tccggtccac ggcggcttcc
    26101 ggcgtgcaga gcagggcgcc gtccgccaca cgatcggcca tggcgcccca gtcgcgctgc
    26161 agcgcttccc gctggcgctc gaggtcccgg gcatccatgc ccaggttgaa catcacgttg
    26221 gctgcgcgac gcagagaggc gggatcccgc gcctcggcct cgcaccagtg attgagaacc
    26281 tcgttgactc tggcaaactc ctccggtgac aggtacgcgg cgttccagcc gtccgcgtag
    26341 tcggcaacga gcttcagcgt ccgcttttcg ccgacgccgc cgatccagat cggcaggcgt
    26401 tcctggaccg gcttcggcag gcagctggcg tcgtcgacct ggtagtgctt gcctgagaag
    26461 ctggtgcggt cctgggtgag catcccgcgg atgatctgcg cagcttcctc gagcatgtcg
    26521 agccgcgtgc cgatgtccgg aaaagggtag ccgtatgccg aggcttccca gacgtgccag
    26581 ccggcgccga tgccgagctc gaaccgccct gaggtgatgt gatccagagt ggtcgccgcc
    26641 ttggccagca gcgccggatt gcggtacccg acgtagaaaa cgaggcagcc gatgcgcgcg
    26701 ttgcttgttt cggcggccag cgcgccgagg gtggtcagag cctcgaagtg cggctctgtg
    26761 ccgcccttgg gcggggcctc gtagaagtga tcccacgcgg aaatccaatc cacgcctttg
    26821 tcgtcgagtt tgcgccagag cgcgcgcatg tcgttcatgg ccatgttttg ctggcccgcg
    26881 tggacgccca acgagatgct catccctgcc tctaccggtt cagtgctgcg ccggcaggat
    26941 acatgagcgg gagggcggac ggaattcgtc gagttgccac tgtcggggcg ctcgagctgg
    27001 tcggccgtcg cttacgaact gatacagtct cgaatcaagc ggttacaaga tcgatgtggc
    27061 gtttcggcgg ttttatcgga actcttacgc cgaatatcgg acaaagtgta gtgagagctg
    27121 agccatttgc tataaacgat ctcgaacggt ttggagatga tatccagcgt taagaaagaa
    27181 aagatccaca acgtctgacg agtttctgag taccccggcg gcaacctccc ctcccctccc
    27241 caatcccttg ccgccggggt cttttttttg gccggcaaag accctcaggg tgctgcagga
    27301 ccggcgggtt tcaggcgccg gtgggctgcc gggttcggct gacgagcgcg cgcagctggg
    27361 cggcgagatc cttgagctga tgggggagtg ccagcagcgc gggcgttgcg atcagcgtca
    27421 gcagcgttgc gaaggtcagt ccggaaacga tggcctggga tagaggcacc cagaagcccg
    27481 acagctgacc gccgtagatg atgctgcgat tcaccaggtc gatggacagg ttgctggcaa
    27541 gcggcagcag cccgaagatc gtcgttaccg tcgtcagcat gaccgggcgc agccgctgag
    27601 cgccggttcg aacgatgagc gtcacgtggt ccaggtccgg atgctggcgc cggaggtggt
    27661 tgtacgtgtc gatgaggacg atgttgttgt tcacgacgat gccggccagg gccacgatac
    27721 cgacgccggt gaggatggcg ctgaagggtg cctgcaggat caacagaccg agcagcacgc
    27781 cggcggtgga catgaccacg gcgaccagga tcagcgcgct ctggtagatg ctgttgaact
    27841 gggttaccag cagcacgaac atgagcagca gggacaggcc gaaagcgacc tgaacgaacg
    27901 ccagggactg ggcctgctct tcgttggcac ctcgaaagtc gatgtccagg tcggggtcga
    27961 aagtctgcgc gctcagccag tcgcgaatca gcgtcacctg atcgtcggca agaacgcctt
    28021 cggctacgtt ggcgcggatg aactccaccg ggacgccgtc gattcgctga aacgtgtcga
    28081 cgttgggggc gggctcccgc ttcacgaagt tggaaatggg gatcagccct ttggtcgtgc
    28141 ttatcttcag gtcgtccagg gcgctgatgc cgcgagcgtc gctcgggtac ctgacccgga
    28201 tgtccaccgc atcgtccgcg cgctccggac gatactcgcc gactttcacg ccgttcgtga
    28261 ccagctgcac ggcaatgccg acctgggaaa cgtcagcgcc gtacagggcg gcctgggcgc
    28321 gatccacgga cagtttccat tcgatgccag gcagggatcg cgtgtcgtcg atgtccagca
    28381 gcccgggcaa ggtttccatg tactcgcgca ccctcgtaac ggcgggttcc agcagctcac
    28441 ggtcgtagga agaaaactgt atctgcaccg gtttgcccac cggagggccc tgctcgagct
    28501 tctgcacctc gacggcaatg ccggccaggc cggcggtgcg ttgccgtatc tccgcgaaga
    28561 tttccgcacc cttgcggtcg cgctcgctct cggggtgcag ctcgaggaac atggagccca
    28621 cgcggtcgaa accgccccga gtggagccgc cgctcaagag cgtgtaggtg ttgacggttc
    28681 gtatgcccga aacctcgagg atctcgctgt ccacctcgcg gaccagagcg tcgatttcat
    28741 cggctgacag gttgccgcgg gcgcgcacgg caacctgggc gaactgggga tcgctgtctg
    28801 agaagaacac catgcccttc ccgtaggcgc cgtaccccca gaagatggtg accaggatga
    28861 ccaggatgcc ggagagggtg gccagggcgt gtcggctggt gatggtcagc agccgcgcgt
    28921 accagccggt cacgctcttc agacgcgtgg gatcgccgtc ttccagctcg ttgagcgtcc
    28981 tctgcgcttt ggcgtcgcgc gcgcccgcct tgccgaacag ggaacccagc acggggccga
    29041 atatcagcgc gtagatcagc gatcccgaaa gcacggtgag aacggtgacc ggcagatagc
    29101 tcatgaactt gcccggtatt cccggccaga acatcagcgg caggaaggct gccagggttg
    29161 tcgcgacgga tgcggtgacg ggccagaaca tccgtttggc ggccatggca taagccgcgc
    29221 ggcgctcgaa gccttcggtc atcttgcggt ccgcgtactc ggtcacgacg atggccccgt
    29281 cgatgagcat gcccagaccg aggagcatgc cgaacatgac catgaagttg aacgtgtacc
    29341 ccagcaggta gatgaagatc agcgagaaca ggaacgacac cggtatggcc agcccgacga
    29401 tcacgccgct gcggaaaccc atggcggcca cgaccagaac catcaccagc gcgagcgcag
    29461 tgaggatgtt gccctgcagc tcggtgacct gtccctcggc gaagggggcc tggtcctggg
    29521 agaagaagac gtcgacccgg ttcggcaggt cgggccgatg cctctcgacg acggctttga
    29581 ccgcatcgat ggtgtcgatg atgttcgcgt tggcgcgctt ggtgatctgc aatgaaagcg
    29641 ccgcgttgcc gttcacgcgc gcgtagctgg tccggtcctt gaacgtgcgg cgcacgacgg
    29701 ctacgtcgtc gagggttacc accgtgtctc cgctggcccg tactggcagg tccagaacgt
    29761 cctctgcgcg ttcgatgatg ctgggaatct tgacggcgaa tctgccttcg ccggtgtcga
    29821 tgctgccggc cgggatcagc cggttgtttc gaacgatcgt ggtgatcagc tgctcgttgg
    29881 aaatggcgta tgcttccagc gcggcgggat cgatcaccgc ctcgagcatt tcctcccggt
    29941 ggccccggag ctcggct
//
LOCUS       DBNHLM010000287         4157 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1658423, whole genome shotgun sequence.
ACCESSION   DBNHLM010000287 DBNHLM010000000
VERSION     DBNHLM010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1658423"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="AC16QF_13375"
     CDS             complement(<1..1135)
                     /locus_tag="AC16QF_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR6449598.1"
                     /translation="MKRMLINATQPEEVRVALVDGQKLFDLDIESRGREQKKASIYKA
                     KITRVEPSLEAAFVDFGADRHGFLPLKDISREYFKQKPPEGGKLIIQDLVSEKQEILV
                     QVDKEERGTKGAALTTFISLAGRYLVLMPNNPRAGGISRRIEGDDRDQLRDALGQLNV
                     PEGMGVIVRTVGVGRSPEEMQWDLDYLLQLWEAIQKADEQERAPKLIYQENNVILRAI
                     RDNLRKDIGEVLIDGKEAFEEAKDFIEQVMPHYSQRVKYYSDPIPLFNRFQIESQIEM
                     AFQHSVRLPSGGSLVIDPTEALASIDINSARATKGADIEETALNTNLEAADEIARQLR
                     LRDMGGLIVIDFIDMTSVKNQRAVENRMREALDADRARVQISRI"
     gene            1647..2624
                     /locus_tag="AC16QF_13380"
     CDS             1647..2624
                     /locus_tag="AC16QF_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR6449599.1"
                     /translation="MSQTLEEKSSTSVRYLDIGEEAGQRIDNYLMARLPGVPKSRIYR
                     IIRRGEVRVNGGRIKPTYRLSAGDRVRVPPLHLATRPPPAVGQRDLARIEAAIIFENE
                     RLLVLNKPAGVAVHGGSGVSYGIIEGLRRLRPEARLELAHRLDRETSGCLLVAKGRQS
                     LLEIHAALRERTVRKRYEVIVHGRWPGNVRTVRLPLKKYSTSSGERRVRVDAQGKPSR
                     TDFEVVGRSEAATWLVAHLHTGRTHQIRVHAKASGHEVVGDEKYGKPEQRALEGRLGI
                     SGMCLHAEGITLVLNGEKYKFSCPVPDTFAAAWERLSEDVDPLGSEHDA"
     gene            complement(2580..3161)
                     /locus_tag="AC16QF_13385"
     CDS             complement(2580..3161)
                     /locus_tag="AC16QF_13385"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012075171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR6449600.1"
                     /translation="MSTIVLASSSKYRAALLARLQLPFETADPAIDEAVQKGEAPEDL
                     VRRLAQAKAEAVAARFPRALVIGSDQVAVCDDHILGKPGTEARAREQLAKLSGRRVRF
                     VTGLCLLNATQTRSQLAVVETPVHFRELSEREIADYVMREQPVDCAGAFKSEGLGIAL
                     FREIGGPDPNALIGLPLIELCVMLRSEGVNVLG"
     gene            3282..3797
                     /locus_tag="AC16QF_13390"
     CDS             3282..3797
                     /locus_tag="AC16QF_13390"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR6449601.1"
                     /translation="MKTRPQEALRYVELAQRGARFERSLGGADLPRWSEVVEGEVAVD
                     VSLSFAQDEASRPCVKGSYRARSDMLCRRCSEVLPYEWAGDFELTIVGDETAASQLGV
                     EHDVLLVEGEELEVAEILEDELLMAMPDQLCREEPCERMMPREFPAPGGATESAAERG
                     NPFAAIAKLKD"
     gene            3816..3992
                     /gene="rpmF"
                     /locus_tag="AC16QF_13395"
     CDS             3816..3992
                     /gene="rpmF"
                     /locus_tag="AC16QF_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR6449602.1"
                     /translation="MAVQKNKVTRARRGKRRSHDALRAPALSIDPTTGETHRRHHVTA
                     DGFYRGRKVLEQED"
BASE COUNT          746 a         1222 c         1357 g          832 t
ORIGIN      
        1 aaatcctgct gatctgaacc cgggccctgt ccgcatcgag ggcctcccgc atgcgattct
       61 cgaccgctcg ctggttcttc acgctggtca tgtcgatgaa gtcgatgacg atcaggccgc
      121 ccatgtcccg cagacggagc tgccgggcaa tctcgtcggc ggcttcgaga ttggtgttca
      181 gcgcagtttc ttcgatatcg gcgcccttgg ttgcacgggc cgagttgatg tcgatggatg
      241 cgagggcttc cgtggggtcg atgacaaggc tgccgccgga cggcagcctc accgaatgct
      301 ggaaagccat ctcgatctgg ctttcgatct gaaaacggtt gaagagaggg atcggatccg
      361 agtagtactt cacccgctgg ctgtagtggg gcatcacctg ctcgatgaag tctttggctt
      421 cctcgaaagc ttccttaccg tcgatcagca cttcaccgat gtccttgcgc aggttgtcgc
      481 ggattgcgcg caggatgacg ttgttctcct gatagatgag ttttggagcg cgttcctgct
      541 cgtcggcctt ctggattgcc tcccagagct gcagcaggta gtcgaggtcc cattgcatct
      601 cctcggggct tcgacctacc ccgacggtgc gcacgatgac gcccatgccc tcggggacgt
      661 tgagctgacc gagcgcatct cggagctgat ctcggtcgtc gccttcgatg cggcgggaga
      721 tgccgcccgc cctggggttg ttgggcatca ggacgagata ccggccggcg aggctgatga
      781 aggtcgtcaa ggcagcgcct ttggtgccgc gttcttcctt gtcgacctgg accaggattt
      841 cctgtttttc gctcaccaga tcctggatga tcagcttccc gccttcgggc ggcttctgct
      901 tgaaatactc gcgcgaaatg tctttcaggg gcagaaagcc atgcctgtcc gcaccgaagt
      961 ccacgaaggc ggcctccagg ctcggctcta cgcgggtgat tttggctttg tagatgcttg
     1021 ctttcttctg ctctcgtccc cggctctcga tgtccaggtc aaaaagtttc tgaccatcta
     1081 cgagggcaac ccgaacctct tccggctggg tcgcgtttat cagcattctt ttcatctaag
     1141 tcatttcctt agtgccgcgg gctacgcgct gcaccggaaa ttgagagaaa ggacgatggt
     1201 gatgggaggt gcaccagatc aggttgccgc cagccttatc gcggcgctca tggcgctctg
     1261 cgttgctggc cgcgctcagt tgcgctgttt ggctatctgt ctgatctgtc ctgcgtgctc
     1321 caaacctgtc gctcttcgga aaggggcggc aatgccgctg ctctgtcgtc tgtcccgcct
     1381 gatgttcggc ggggtccgcc atgcccttgc atggtggctc ggtattccgt tctcaatttc
     1441 ccggcagccc catggaccgc gttcttcgag agccttggtg tagtcggctc tccatttgca
     1501 attagccgta ttgttggctt aattccgttt aagttgcgcc cggttaggtg ggcgatcgat
     1561 caaaaaagcc ggctgaacag tttagaaaca agtaacctgc cagccagata ccctgtgggg
     1621 cgaccaatat tagcagtcat cattaagtgt ctcaaacact tgaagaaaaa tcgtcaacgt
     1681 cggttcgcta tctggacatc ggcgaggaag cgggccagcg aatagacaac tatctcatgg
     1741 ctcggctgcc gggcgttccc aaaagccgca tatatcggat cattcgccgt ggcgaggttc
     1801 gcgtgaatgg cgggcgtatc aagccgacct accgtctatc ggccggcgac cgggtccggg
     1861 tgccgccgct gcaccttgct acccggccac ccccggcggt tgggcagagg gaccttgctc
     1921 ggatcgaggc cgcgatcatc ttcgaaaatg agcgcctgct ggttctgaac aagcccgccg
     1981 gcgtagccgt gcacggcggg tcgggcgttt cctacggaat aatagagggc ttgaggcggc
     2041 tcaggccgga ggctcgcctg gagctcgcgc atcggctgga ccgggaaacc tccggctgtc
     2101 tgctggtggc gaagggccga cagtcgctcc tcgaaataca cgctgctctg cgcgaaagga
     2161 cggtgcgaaa gcgctacgag gtaatcgtgc acgggcgctg gccgggcaat gttcgtacgg
     2221 tgcggctgcc tctgaagaag tactcgacct ccagcgggga gcgccgggtc cgggtagacg
     2281 ctcagggaaa gccgagccgc actgacttcg aggtggtcgg gaggtcggag gcagcgacct
     2341 ggctcgttgc ccatctgcac accggccgta ctcatcagat tcgcgttcac gccaaggcca
     2401 gcgggcatga ggtcgtcgga gacgaaaaat acgggaagcc ggagcaaagg gcgctggagg
     2461 gccgcctggg gatctcgggg atgtgtcttc acgcagaggg gatcacgctc gtgctgaacg
     2521 gcgaaaagta caagttcagc tgcccggttc cggacacgtt tgccgccgct tgggaacggc
     2581 tatccgagga cgttgacccc ctcggatcgg agcatgacgc atagctcgat gagaggcaga
     2641 ccgatcaggg cgttggggtc tggcccgccg atttcccgga aaagagcgat cccgagcccc
     2701 tccgacttga atgcgcccgc gcaatcgaca ggctgctcgc gcatcacgta gtcggcgatc
     2761 tcccgctcgc tgagctcgcg aaagtgcacg ggcgtttcca cgaccgcaag ctgggaacgg
     2821 gtctgcgtgg cgttgagcag gcacaagccg gtcacgaacc gcacgcgtct ccccgacagc
     2881 ttggcgagct gttcgcgggc ccgcgcctcg gtgcccggct tccccaggat gtggtcatcg
     2941 cagacggcaa cctgatcaga gccgatcacc agagcccgcg ggaagcgggc ggcaaccgcc
     3001 tcggccttgg cctgagcgag ccgtcggacc agatcttccg gagcttcccc cttctgcacg
     3061 gcctcgtcta tggccgggtc ggccgtttcg aaaggcagct gaagacgggc aagcagcgcg
     3121 gcgcgatact tcgacgacga tgccaggacg atggtgctca aggcccgtat cttcgcagcg
     3181 cgctgacgga aaatgccaga aattttgggg gcttagcgta caagcggtcg ctcgttgcgt
     3241 tctttacagg ggcgggtagg gtacctatga ttgcgcccgc tatgaagacc cgcccccagg
     3301 aggccctgcg ctatgtagag cttgcgcaga gaggcgcccg cttcgagcgg tcacttggcg
     3361 gtgcggatct gccccgctgg tcggaggtgg ttgaaggcga ggtcgccgtc gacgtgtctc
     3421 tgagctttgc gcaggacgaa gcgtcgagac cgtgtgtaaa ggggagctat cgggcaaggt
     3481 cggacatgtt gtgccgacgc tgctcggagg tgctgccgta cgagtgggcg ggcgacttcg
     3541 agctcaccat cgtcggtgac gagacggcgg cgagccagct gggtgtggag cacgacgtcc
     3601 tcctcgtaga gggggaagag ttggaagtgg ccgagattct cgaggacgag ctgctgatgg
     3661 cgatgcccga tcagctctgt cgagaggagc cgtgtgagcg aatgatgccg cgcgaatttc
     3721 ccgcgcccgg cggggcgacc gaatcggccg cagagcgcgg caacccgttc gcggcgatag
     3781 ccaaactgaa ggattgaacg agaaccggag gccagatggc tgttcaaaag aacaaagtga
     3841 cccgagcgcg gcgcggtaag cggcgctcac atgatgcgct tcgcgcaccc gctctctcca
     3901 tcgacccgac gacgggagag acccatcgcc ggcatcacgt gacggcggac gggttctacc
     3961 ggggcaggaa agtcctcgag caggaagact gacgcaccgt gagtctcggt ttcatgttcc
     4021 cggggcaggg ctcccagacc gttgggatgc tctcggacgt cgccgccgaa tacccgcagg
     4081 tgacggagca ttttcggcgc gctggagact gcatcgagcg gccgctgtgg gaaattgcca
     4141 cgtcgggacc cgatacg
//
LOCUS       DBNHLM010000288         3324 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1297932, whole genome shotgun sequence.
ACCESSION   DBNHLM010000288 DBNHLM010000000
VERSION     DBNHLM010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1297932"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="AC16QF_13400"
     CDS             complement(<1..337)
                     /locus_tag="AC16QF_13400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR6449603.1"
                     /translation="MTENEDVDAFRELIEAAGNVVFFTGAGISTESGIPDFRSPGTGL
                     WNKIRPIEFQDFVASEDKRREYWQRHFTGERPMANAKPNKGHDGVQRLIASGKATAVI
                     TQNVDNLHQD"
     gene            complement(404..1048)
                     /locus_tag="AC16QF_13405"
     CDS             complement(404..1048)
                     /locus_tag="AC16QF_13405"
                     /inference="COORDINATES: protein motif:HMM:NF019544.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol phosphotransferase CPT family
                     protein"
                     /protein_id="HIR6449604.1"
                     /translation="MQIPSPRMQTGKIIFLNGSSSAGKTTIAVTLQQLLEEPFQHIAL
                     DQFRDGMPGRFRGLNSPEGTPGARGLNVVPVERDGEKMTEIRFGEVGEQILRGMRRAI
                     SAFAAAGNNVIVDDLLFKPDYLHDYAKALADHDAWLIGVRCSLDVVNEREAQRPGRFP
                     GTATSHFHQVHEHGAQYDLEVDTTTTTPRACAQLIIERLARPPEALERLRSARP"
     gene            1036..1743
                     /locus_tag="AC16QF_13410"
     CDS             1036..1743
                     /locus_tag="AC16QF_13410"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase family
                     protein"
                     /protein_id="HIR6449605.1"
                     /translation="MVSAPMLHHNSGMQGEPGQALAAELDIRIPRMDGLSLRVAPVAD
                     FEHELHGPELEAVARAVPKRRQEFAPGRHLARAAMRELGLPAAAVPRRADRSPAWPDG
                     CLGSITHTNALAGVAVARAGVVEGIGIDLEESHRVSAELHDKLFTPTELGRYVDAPPG
                     WADLLFSAKESVYKAVNPIVGKYIGFHEVEVDVTFAEQRFSARYVGEHAPNALLENGA
                     GYFLFAEQHVFSVFIIP"
     gene            1770..3008
                     /locus_tag="AC16QF_13415"
     CDS             1770..3008
                     /locus_tag="AC16QF_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HIR6449606.1"
                     /translation="MQAILGIGFLLGLAWILSESRRTVRWRPVMVGLVVQLGLAALLL
                     RVPVVAESLLVLNKLVYAVEAATGAGAGFVFGYLGGGDAPFTVSAQGAMYVFAFRVLP
                     QILVFSVLVALLWYWRVLPAVIRGFGYLLRRALDVGGAVGTAAASSVFLGMVEAPLVI
                     RAYLERLSRSELFTVMTCGMSTVAGSIMVLYASVLGDVITGALGHILAASVINVVGAV
                     VVSRIMIPADDATAEEDGASGLSYVSNMDAITRGTADGLRLAVNVGAMLVVLVSLVAL
                     VNQGLGAVTVGEGPLSLERMMGWVFSPVAWLIGIPWGEAQAAGALLGTKLILNELVAY
                     LQLAGLPEGSLSDSSRLVMTYALCGFANFGSLGILLGGLNTLGPERRKEILSIGPKTL
                     ISGTIVTCNTGAIVSLIGLF"
     gene            complement(3029..>3324)
                     /locus_tag="AC16QF_13420"
     CDS             complement(3029..>3324)
                     /locus_tag="AC16QF_13420"
                     /inference="COORDINATES: protein motif:HMM:NF013384.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HIR6449607.1"
                     /translation="AGILAAGGIYALNHHVDRLADDHSRAARLAQAVNERYPDGAEAH
                     TNMVFLNLAEAEVDRLKDHLADRGIVIRGPRWVTHLDVSDEDVARVVDAIRAS"
BASE COUNT          489 a         1043 c         1131 g          661 t
ORIGIN      
        1 agtcctggtg caggttgtcc acgttctggg tgatcacggc ggtcgccttg cccgaggcga
       61 tcagccgctg aaccccgtcg tggcccttgt tcggtttggc gttggccatg ggcctctcgc
      121 cggtgaagtg gcgttgccag tactcccggc gtttgtcttc gctggcaacg aagtcctgaa
      181 actcgatggg tctgatcttg ttccacaggc cggtgcccgg gctgcgaaaa tcgggaattc
      241 ctgactcggt gctgataccc gcgcccgtga agaacaccac gttcccagcg gcttcgatca
      301 gctcgcgaaa cgcgtcgaca tcctcgttct cggtcattgg cctgcctccc tgtggggttg
      361 tcgcgtgttc agccccgcgt tgtcgattgc cgatgtccca ggatcagggt ctggccgagc
      421 gcagtcgctc gagcgcttcc ggcggccggg ccagacgctc gatgatgagc tgcgcgcacg
      481 cgcgcggggt cgtcgtcgtg gtgtcgacct ccaggtcgta ctgcgcgccg tgctcgtgca
      541 cctggtgaaa gtgcgaggtg gcggtgccgg gaaagcgccc ggggcgctgc gcttcgcgtt
      601 cgttgaccac gtcgaggctg cagcgcacgc ctatcagcca ggcgtcgtga tctgccaggg
      661 ccttcgcgta gtcgtgcagg tagtcgggct tgaagagcag atcgtcaacg atcacgttgt
      721 tgccggcggc ggcaaaggcg gagatcgccc gacgcatgcc tctcaagatc tgctcaccca
      781 cctcgccgaa gcggatctcg gtcatcttct ccccgtcgcg ctctaccgga acgacgttca
      841 ggccccgggc cccgggcgtt ccctcggggg aattcagccc gcggaaacgg cccggcatcc
      901 cgtcgcggaa ctggtccagg gcgatgtgct gaaagggctc ctcgagcagc tgctggagcg
      961 tgacggcgat ggtggtctta ccagcgctcg aagagccgtt cagaaagatg atcttgccgg
     1021 tctgcatacg cggcgatggt atctgcaccc atgctgcacc acaattcggg catgcaggga
     1081 gagcctggac aagcgttggc ggccgagctc gacatccgca taccgcggat ggatggcctg
     1141 agcctgcgcg tcgcccccgt ggcggatttc gagcacgagc tccatggccc ggagctagag
     1201 gctgttgccc gggccgtgcc aaagcggcgg caggaattcg cccccggccg tcatctggcc
     1261 cgtgcggcca tgcgcgagct cggcctgccg gccgccgcgg tcccgcgccg ggccgaccgc
     1321 agccccgcat ggcccgacgg gtgccttggc agcatcaccc acaccaatgc gctggccggc
     1381 gtcgccgttg cccgagccgg cgtcgtggaa ggcatcggca tcgatctcga ggaatcgcac
     1441 cgggtgtctg ccgagctgca cgacaagctc ttcaccccga cagagctcgg ccggtacgtg
     1501 gatgcgccgc cggggtgggc cgatcttctg ttcagcgcca aagaatccgt ctacaaggcg
     1561 gtcaacccga tcgtcggcaa gtacatcggg tttcacgagg tggaggtgga tgtgacgttc
     1621 gcagagcagc gcttttctgc ccgctacgtg ggcgagcacg ctcccaacgc cttgctcgaa
     1681 aacggcgcgg gctacttcct tttcgccgag cagcacgtct tcagcgtgtt catcattccg
     1741 tgaaccccta cctcgaaggc agagtctgaa tgcaggccat actcggaatc ggctttctgc
     1801 tggggctagc gtggattctg agcgagagcc ggcgcaccgt gcgctggcgg cccgtgatgg
     1861 tgggtctcgt cgttcagctc ggccttgccg cgctgctgct gcgggttccc gtggtggccg
     1921 aatctctgct ggtgctcaac aagctcgtct atgcggtgga ggcggcgacc ggggccgggg
     1981 cggggttcgt gttcggttat ctgggcggtg gggatgcgcc gttcacggta agcgctcaag
     2041 gggccatgta cgtctttgcg tttcgcgttc tgccgcagat cctcgttttc agcgtgctcg
     2101 tggcgctgct gtggtactgg cgcgtgctgc cggcggtgat ccgcgggttc ggctacttgc
     2161 tccgtcgtgc actcgatgtg ggcggcgccg tgggaacggc ggctgcctcc agcgtgtttc
     2221 tcggcatggt ggaagcaccg ctggtgatcc gggcctatct cgagcgcttg tcccgttcgg
     2281 agctcttcac ggtcatgacc tgcggcatgt ccaccgtggc cggatccatc atggtcctct
     2341 acgccagcgt gctcggcgac gtcattacgg gcgcgctcgg gcacatcctg gccgcttcgg
     2401 tgatcaacgt ggtcggcgcc gtcgtcgttt cgcgcatcat gatcccggcg gacgacgcca
     2461 cggcagaaga ggacggcgct tccggtctca gctacgtctc caacatggac gccatcacgc
     2521 gcggcacggc ggacggtctg cgtctggccg tgaacgtcgg cgccatgctc gtagttctcg
     2581 tgagccttgt cgctctggtg aatcagggcc tgggcgcggt gacggtgggg gagggcccgc
     2641 tttctctgga gcggatgatg ggatgggtgt tctcccccgt agcctggctg atcgggattc
     2701 cctggggaga agcccaggcg gccggggcgc tgctcggcac caagctgatc ctgaacgagc
     2761 tggtggccta tctgcagctc gccgggctgc cggagggaag cttgagcgac tcgagccggc
     2821 tggtgatgac ctacgcgctg tgcggctttg ccaatttcgg cagcctgggc attctgctcg
     2881 ggggcctgaa tacgctcggt ccggaacgac gcaaagagat attaagtatc ggacctaaga
     2941 cgttgatttc cggtacgatt gttacctgca acaccggggc aatcgtgtcg ctcatcggcc
     3001 tgttctgaag cggtaagacc aaggcgcctc agcttgcccg aatggcgtcg acgacgcgcg
     3061 cgacgtcctc gtcggagacg tcgagatggg tcacccagcg cggcccgcgg atgacgatgc
     3121 cacgatcggc cagatggtct ttaagcctat ccacttccgc ctcggcgagg ttcaggaaca
     3181 ccatgttggt gtgtgcttca gcaccgtcgg ggtatcgctc gttgacggcc tgcgccagac
     3241 gcgcggcccg tgagtggtcg tcagccaggc gatccacgtg gtgattgagc gcgtaaatgc
     3301 ccccagcggc gaggatgccg gcct
//
LOCUS       DBNHLM010000289         3456 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_222308, whole genome shotgun sequence.
ACCESSION   DBNHLM010000289 DBNHLM010000000
VERSION     DBNHLM010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_222308"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="AC16QF_13425"
     CDS             complement(<1..348)
                     /locus_tag="AC16QF_13425"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6449608.1"
                     /translation="MSWCSALCALLVVSPPSIAAEGETGSFVEEVVVTAQKREQSIND
                     VGMSIDVATGEKLSDLGISDTADLGKVVSGFNANTNYYGSYVYTIRGIGFQDTALASS
                     NTVSVSIDEMPIPF"
     gene            complement(687..2105)
                     /locus_tag="AC16QF_13430"
     CDS             complement(687..2105)
                     /locus_tag="AC16QF_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019751080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6449609.1"
                     /translation="MQTYDKFYINGEWVAPHGTGTIEVIDPSTEEVCGVVPSGDDVDV
                     DAAVAAAKAAFKTWSRSSAAERSEIIGKLADKITENMAKIGELCARELGTPLQASIAT
                     HGGLGIGVMSSYKEIPFEMEKEEQIGNSIVVKEPIGVCAFITPWNFPLHQIVAKLAPA
                     LAAGCTVVVKPSSDTPLTAYFLAEMIEEIGVPAGVFNLITGPGRTVGEAMCTHPDVDL
                     VSITGSTEAGIRVAQLAAGTVKRVCQELGGKSPLVALEDADISATAARAAGGICFNSG
                     QVCAALSRLIVPRGKQDEAIAAAKAAAESVVVGGAFEEGVTMGPVASKSQFDSVRAYI
                     EKGIEEGATLVTGGTEMPDGRNAGYFVKPTIFAAVRNDMTIAQEEIFGPVLCIIPYDS
                     EEEAVEIANDTVFGLSGAVDSGDPERAKAVARQIRTGQVSVNGGAFNVAAPFGGYKQS
                     GNGRELGPHGLAEYVELKSMQT"
     gene            complement(2243..>3456)
                     /locus_tag="AC16QF_13435"
     CDS             complement(2243..>3456)
                     /locus_tag="AC16QF_13435"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6449610.1"
                     /translation="KLPGLANSWTMPIKARIDMLSTGIKTPVGIKVSGPDLEVLQRLS
                     QEIEIVMSSMPETLSAFGDRAVGGYYMDSDIDRSQAARYGLTVGDVQDVIQSAIGGMN
                     VTATVEGLERYPVNVRYPRELRDTPDALRRVLIATPTGVQIPLEAVADISMNRGAPVI
                     KSENGRPNAWIYVDPRTSDIGGFVAKAQRLVAQQVAIPAGYTIRWSGQYEYMERARTR
                     LQILVPVTLLAIFLLLYGHFRNVLAPAVVMLSIPFGLIGGVWLTFALGYNLSVAVAVG
                     FIALAGMAAEIGVLVLSFIDAEIARLRGDGGRLSAAAIREAARSATAQRVRPVVMTAA
                     STMAGLLPIMLSSGTGSDVTHRIAAPMLGGMLTVMILNLLVLPVIYSLALQHRERRLG
                     SEGTRQSESSA"
BASE COUNT          628 a         1147 c         1043 g          638 t
ORIGIN      
        1 gaaggggatc ggcatctcgt ctatgctgac gctgacggta ttggatgacg cgagcgccgt
       61 gtcctggaag ccgattccgc gaatcgtgta aacgtagctg ccgtagtagt tggtgttcgc
      121 gttgaacccg ctgacgacct tgccgagatc ggcggtgtcg ctgatgccga ggtcagagag
      181 cttctctccc gtggctacgt cgatggacat accgacgtcg ttgatgctct gctcccgctt
      241 ctgcgcggtc acgaccacct cttcgacgaa agatccggtt tcgccttcgg ccgcaattga
      301 aggcggcgag acgacaagca gcgcgcaaag ggcgctgcac caactcataa gcgccctacc
      361 agggctcgtt ccccgattca ccaattcgac ttgaattatc cgtcccgaca tggctctctc
      421 cccgtgagtc tcatgaccaa tctgatcact aacgcaataa gggcagcctt ccagaaaacc
      481 gcacagcctt gcagcatctg caaatccaaa cgctctcagg ctgacgattc tcaatatccc
      541 taccctccct tggaggatgc aggaaacgaa ttcgattgtc tattaccgag ccttgaacgc
      601 cgcgactgcg gaccactcgt cagaaaggag gagcgctccg cctgcaacca tcaatgtcac
      661 aggcggggcg cacgtggagg gttctcctag gtctgcatgg atttcagctc gacgtactcc
      721 gccaggccgt ggggaccgag ctcgcgcccg ttgcccgact gcttgtatcc gccaaagggt
      781 gccgccacgt tgaacgcgcc accgttgacc gacacctgcc ccgtgcggat ctgccgtgcg
      841 accgccttgg cccgctcggg atcgccagag tccaccgcgc cggacaggcc gaagacggtg
      901 tcgttggcta tctcgaccgc ctcttcttcg gagtcgtaag gaatgatgca gagcaccgga
      961 ccgaatatct cttcctgggc gatggtcatg tcgttgcgga cggccgcgaa gatcgtcggt
     1021 ttcacgaagt agccggcgtt gcgcccatcg ggcatttccg tgccgcccgt gaccagagtt
     1081 gcgccttcct cgataccctt ttcgatgtac gcccgcacgg agtcgaactg cgacttggaa
     1141 gccacgggcc ccatggtcac cccttcctcg aaagcgccac cgaccaccac ggattccgct
     1201 gccgccttgg ccgccgcgat ggcttcatcc tgcttcccac ggggcacgat gagccggctg
     1261 agcgcggcgc agacctgccc gctgttgaag cagatgccac cagccgcccg ggcggcggta
     1321 gccgagatgt ccgcatcctc cagggcgacc aggggcgact tgccaccaag ttcctggcac
     1381 acgcgcttga cggttcccgc tgcgagctgg gcgacccgaa tgccggcttc cgtggatccc
     1441 gtgatggaga ccaggtccac gtccggatgc gtgcacatgg cctctccgac cgtcctgccg
     1501 ggacccgtga tcaggttgaa cacaccggcc ggcacgccga tctcctcgat catctcagcc
     1561 agaaagtagg ccgtcagcgg cgtgtcggaa ctgggcttga ccaccacggt gcagccggcc
     1621 gccagagccg gcgccagctt ggcgacgatc tgatgaagcg ggaagttcca cggggtgatg
     1681 aaggcgcaaa ccccgatggg ttccttgacg acgatggagt tgccgatctg ctcctccttc
     1741 tccatctcga agggtatttc cttgtacgag ctcatcaccc cgatgcccag cccaccgtgg
     1801 gtggcgatgc tcgcctgcag cggggtgccc agctcacggg cgcagagctc gccgatcttg
     1861 gccatgttct cggtaatctt gtccgccagc ttcccgatga tctcggaacg ctcggcggcc
     1921 gacgatcgcg accaggtctt gaaagccgcc tttgccgcgg ccaccgccgc gtcgacgtcg
     1981 acgtcgtcgc ccgacggcac gacgccgcag acttcctccg tggacggatc gatgacctcg
     2041 atggtcccgg tcccgtgagg cgcgacccac tcaccgttga tgtaaaactt gtcgtaagtc
     2101 tgcatattca tctcctactt ggtatctgca gtgctcagca tgggccgtgg attgaaagcc
     2161 cccgattccc ccgaaccttc ggcggcccca gattgccgcc gcacccctga tctcaacatg
     2221 atgccatggc ggttgtaaac agctacgccg acgactccga ctgccgtgtt ccctcggatc
     2281 cgaggcgcct ttcgcgatgt tgcagagcca ggctgtagat caccggcaac accaggagat
     2341 tcagaatcat tacggtcaac atgcctccga gcatgggcgc cgcgatgcga tgagtcacgt
     2401 ccgaccccgt cccgctgctg agcatgatgg gtagcaggcc ggccatggtc gaggccgccg
     2461 tcatcaccac cggacgcacc cgctgtgccg tggcggaccg ggccgcctcc cggatcgcgg
     2521 cagccgacag gcggccgcca tcaccgcgca gacgcgcgat ctccgcgtcg atgaagctca
     2581 gcaccagaac cccgatctcg gcggccatgc ctgccagggc gatgaagccc acagcgacgg
     2641 caaccgatag gttgtagccg agggcgaatg tcagccacac cccgccgatg agtccgaacg
     2701 ggatggaaag catcaccacg gccggtgcga gcacgttgcg gaagtgaccg tagagaagga
     2761 gaaaaatggc cagcagggtc accgggacaa gaatctgcag gcgggtacgc gctcgctcca
     2821 tgtactcgta ctgtccggac cagcggatgg tgtagccggc tgggatcgcg acctgctggg
     2881 ctaccagccg ttgggccttg gcgacgaagc cgccgatgtc ggaggtcctc gggtccacgt
     2941 aaatccaggc gttgggacgt ccattttcgc tcttgatcac cggcgcgccc cggttcatgg
     3001 agatatccgc caccgcctcg agcgggatct gcaccccggt gggcgtggcg atgagcaccc
     3061 ggcgcagcgc gtccggcgta tcgcggagct cccgagggta acgcacgttc accgggtagc
     3121 gctccaggcc ctccaccgtc gccgtgacgt tcatgcctcc tattgcgctc tggatgacgt
     3181 cctgcacgtc gcccaccgtc aacccgtagc gggcggcttg agaccggtcg atgtcggagt
     3241 ccatgtagta gccgcccacg gcacgatcgc caaaggctga aagggtctcc ggcatggaac
     3301 tcatgacgat ctcgatctcc tgggagaggc gctgcaacac ttccaggtcg gggccggata
     3361 ccttgatgcc cacaggggtt ttgatgccgg tggacagcat gtcgatcctc gcctttatgg
     3421 gcatggtcca gctgttggcc aggccgggca gcttca
//
LOCUS       DBNHLM010000290         8834 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_760012, whole genome shotgun sequence.
ACCESSION   DBNHLM010000290 DBNHLM010000000
VERSION     DBNHLM010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8834)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8834)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8834
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_760012"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /locus_tag="AC16QF_13440"
     CDS             <1..1462
                     /locus_tag="AC16QF_13440"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6449611.1"
                     /translation="KMALERPFRSAIELAHLIQTREVSSVELTRTYIERIERYDGAVN
                     AVPVRMFDQALADAAEADAVLARGEDVGPLHGVPMTIKESYVLKDTPATWGLEAFKDN
                     VATRDGLAVSRFRQAGAHFLGKTNVPVDLADFQSYNPIYGTTGNPWNTERIPGGSSGG
                     SSAALAAGFGALEAGSDIGGSIRNPAHYCGVYGHQPTWGIVPRSGHELMEGVPDADLS
                     VCGPLARSAADLALALGIMAGPVEREAVGWRLDLPGPDIASLKSLRVAIWPNDDQAPV
                     SRETEARVVMVGETLERLGATVSDTARPDFDVRKAHINYRSLLESVMGSAKPMAYFEE
                     AQARAADLDPEDMSDEAVTLRGIVMHHRDWIRHNFRRERLRRAWDEFFAEWDVLVCPQ
                     MATPAFPHDHRPFAERTINVDGVEQPYWQQLFWAGMVSNAYLPSTVFPTGPGSDGLPI
                     GLQAISGPYRDYRTIEFASLMAEEIGGFAAPPAMRG"
     gene            1735..2676
                     /locus_tag="AC16QF_13445"
     CDS             1735..2676
                     /locus_tag="AC16QF_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0050896 - response to stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6449612.1"
                     /translation="MKAFEHAIVGLDFNRDCAAAILDRATQLVDPEEIDVVHAFSGND
                     HSPVTAEEAGLMTMCADYGVASVKFVSGESAHALHEQAGKRSDLIIVGSHGRSGLRGI
                     IHSHSNATLHATPCDLLTVHLDDPPQSRSPPYRSVLVAIHLGPVTLPSKCERCSFWPS
                     AQEEDRLNRGHRFMNALKPIKLIPRHSHASEESHYPLCHVPTEVILHARKIAQQSGAE
                     LSLCHVYMGYDVDTQQMERELLDSLGELYEVPEDRRYSVPGSVSGAIHRLASRIGADL
                     VVLGTHGRSGPSLAVGSTANAVLHGATCDQLSVRIGA"
     gene            2874..3578
                     /locus_tag="AC16QF_13450"
     CDS             2874..3578
                     /locus_tag="AC16QF_13450"
                     /inference="COORDINATES: protein motif:HMM:NF015682.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIR6449613.1"
                     /translation="MTELTADLIAELQRLDTPTVCNALEVVAPERRGYGYTVRPLVCT
                     RPELPPLVAVARTATIRAAHPGHLRGSDAQALRDAYYAYVDEGPKPSVMVIQDMDGDQ
                     RGYGSWWGEVNSNVHKGFGCLGVITDGSVRDIPDIAEGFQMLADRIGPSHAYVHPVDF
                     GRTVNVAGMRVNGGDLIHADQHGAVVIPWEVAAEVAAAADTIARRERVIIEAARQPGF
                     DMASLRDAWGDAADIH"
     gene            complement(3594..4922)
                     /locus_tag="AC16QF_13455"
     CDS             complement(3594..4922)
                     /locus_tag="AC16QF_13455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HIR6449614.1"
                     /translation="MNVQPLSVRERMGYGVGDFGVNLFFIPAMTYLLYFYTDVFGLSA
                     AAAGGVFLVARVVDAVTDPLMGGIAERTSSRWGRLRPHILFGALPLGAIAVLTFTTPD
                     LNDAGKLWWAAVTYVLFGIAYTVVSIPYSALTASITADHHERTLLTTVRMGCAFAGGY
                     VVSVGMLPLVGLFETQAAGFAWAMVIFATFATGLLWLTVANTRERVQPPATQKLTVRD
                     SFRAVFANPPLLVVIVLFTGGMLSFTIRQSVTLYYFKYNLGREDLIALFFGITLPVMM
                     VGLLGVPRLAAAVGKAGGIVVGALVTIVGCAGLYLTPYDSVTWVFVWSCVIALGGTPI
                     AVLGWAMIPDTVEYAQWRMGLRADGAIFGFASFFQKLAKAVGGAGVAGALALAGYVAN
                     VEQSEASLRAIHNMMTLAPALIMLVLIIAARAYRLDAATHRRIVQELSGA"
     gene            complement(4930..6129)
                     /locus_tag="AC16QF_13460"
     CDS             complement(4930..6129)
                     /locus_tag="AC16QF_13460"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6449615.1"
                     /translation="MGPLSHIRVLDLTIARAGPSAVRLLADWGADVIKIEPVPGAGQS
                     VTGARRGPDEQNLHRNKRSLCLDLKHPDGHGLFLELAAVADVVVENFKVDVKHRLRVD
                     YETLKQVNESIIYASISGFGQEGPYADRGGVDQIVQGMSGLMSVTGEPGGGPMRVGVA
                     ISDTSAGMFLGQGILLALLHRERTGEGQWVHTSLLEAMMCKLDFQGARYTMNGEVPTQ
                     QGNDHPAYTPMGTFLASDGQVNIAASMPRLWQRFCEALGADDLLAHPDYQTTADRIAR
                     KDQLKEAINAITSQRTSADLVGVLNEAGVPCGSINTIGEGFEDPQVRHLGMAKPAPHP
                     DMGSVNLIRSPINLSRFPQESAFRNAAPDPGFDTRAVLTELGIPSSRIDALAAAGAIA
                     CTDPASA"
     gene            complement(6131..7828)
                     /locus_tag="AC16QF_13465"
     CDS             complement(6131..7828)
                     /locus_tag="AC16QF_13465"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6449616.1"
                     /translation="MSEHELVIRGGTVVDGTGSPERTADVAVRAGVIVAVGEVDGSGR
                     REIRADGALVTPGFVDIHTHYDGQATWSNRLSPSSHHGVTTVVMGNCGVGFAPVKPTD
                     RDLLIELMEGVEDIPGAALHEGIHWEWETFPQYLDYLSQRSFDMDIGAQLPHGALRVY
                     VMGRRGADREAASADDIALMRALTAEAIEAGALGFSSSRTLNHRSSKGAPTPSLTAAR
                     QELLGIARGLKDAGRGVMEMISDFQDADEEFAHLTAMTRESGRPMSISLAQGLSPNGW
                     RSLLGRIASASEAGLPMRAQVAPRPIGILLGLTTTLNPFVTHPAYREIAGLALAERVR
                     AMRDPERRAAILAGSPGRGFQRLWGLMANGAKIWRLEDPPDYEPAPQDSLASRARVAR
                     VDPWEYVYDQLLQAEGRQILYTPFANYAEDNLDCCREMILHPDTVMGLGDGGAHVGTI
                     CDASFVTTLLTHWGRDRTRGERIPLPVLVKSQTADTARAVDLNDRGVLAPGMKADINV
                     IDFERLKVRAPEIVHDLPAGGARLQQKADGYLATIVSGQVTYENGEATEALPGRLIR"
     gene            7924..>8834
                     /locus_tag="AC16QF_13470"
     CDS             7924..>8834
                     /locus_tag="AC16QF_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIR6449617.1"
                     /translation="MQRVTIILIVLSGLILALFAWFPAETLLDAARALDRTPPEPATP
                     DDVTRRTTESGDVVGFIGNHGARTWRGVPYARPPVGALRWRAPEPAEPWPGVREATAA
                     GPLCAQRAGVISGDGFAPTRVAAGSEDCLYLDIYAPANARRLPVMFWIHGGGNSVGHN
                     GPYDGARLATWRDVVVVTVNYRLAHLGWFAHPALTTGNRLHDSGNYGTLDLIQALTWV
                     RDNIQAFGGDPDNVTVFGESAGGTNTLSMLASPRAAGLFHRAIAQSGGLTPMPMARAS
                     AYAEDGGHPSSAREIVNRLLIADGRASS"
BASE COUNT         1485 a         3066 c         2910 g         1373 t
ORIGIN      
        1 aaagatggcg ctcgaacggc ccttccgctc ggccatcgag ctggcacacc tcattcagac
       61 ccgcgaggtc agctccgttg agctgacgcg cacctacatc gagcgcatcg agcgttacga
      121 cggcgccgtc aacgccgtgc ccgtgaggat gttcgaccag gccctggccg acgccgccga
      181 ggccgacgcc gtgctggccc gcggcgagga cgtgggcccg ctgcacggcg tgcccatgac
      241 catcaaggag tcctacgtcc tgaaggacac gccggccacc tggggcctgg aggctttcaa
      301 ggacaacgtg gcgaccagag acggcctggc cgtgagccgg ttccgccagg ccggcgccca
      361 cttcctgggc aagacgaacg tccccgtgga tctggccgac ttccagagct acaaccccat
      421 ctacggcacc acgggcaacc cctggaacac cgagcgcatc cccggcggct cctcgggagg
      481 cagctcggcg gccctggcgg ccgggttcgg cgccctggaa gccggctcgg acatcggcgg
      541 gtccatccgc aacccggccc actactgcgg cgtgtacggc caccagccca cctggggcat
      601 cgtgcccagg tccggccacg agctgatgga gggcgtgccc gatgccgacc tctccgtgtg
      661 cgggccgctc gcgcgcagcg ccgcggatct ggccctggcg ctggggatca tggcggggcc
      721 cgtcgagcgg gaggcggtcg gctggaggct cgacctgccc gggcccgaca tcgcgtcgct
      781 caaatccctg cgcgtggcca tctggcccaa cgacgaccag gctcccgtct cgagagaaac
      841 cgaggccaga gtcgtcatgg tgggcgagac gctcgagcgg ctcggcgcca ccgtctcgga
      901 caccgcgcgt ccggatttcg atgtgcgcaa ggcccacatc aactaccgca gcctgctcga
      961 atccgtgatg gggagcgcca agcccatggc ttacttcgag gaggcccagg ccagggcagc
     1021 ggacctggat cccgaagaca tgtccgacga ggcagtgacc ttgcggggca tcgtcatgca
     1081 ccaccgggac tggatccgtc acaacttccg ccgcgagcgc ctgcgccggg cgtgggacga
     1141 gttcttcgcc gagtgggacg tcctcgtctg cccgcagatg gccaccccgg catttcccca
     1201 cgaccaccgg cctttcgcgg agcggacgat caacgtagac ggcgtcgagc agccctactg
     1261 gcagcagctc ttctgggcgg gcatggtcag caacgcctac ctgccgagca cggtgtttcc
     1321 caccgggccc ggcagcgacg ggctgccaat cggcctgcag gccatcagcg ggccgtaccg
     1381 cgactatcgg accatcgagt tcgcgagcct gatggccgag gagatcggcg ggttcgcagc
     1441 cccgcccgcc atgagaggat gagttggggg cgtaaggcgc cccatatcct cgaccacccg
     1501 ctgctgccag aaccgcgaat ggccgtggcc ccgcatggtg agcccatgcg aaccgttctc
     1561 gcccgcttct gatcaggcag cgatactcca ctgaaacctt cagatttcct aatcgaaaat
     1621 cattcttata ttcatcgggc taattagaaa accgggggtt attccaaagg ggtcgttcag
     1681 accatggctt gagggtgtta gactgtttgc tactacccaa acggacgcgt tggcatgaaa
     1741 gcctttgaac acgcgatagt cgggttagat ttcaaccgcg attgcgcggc ggcgatcctt
     1801 gatcgggcca ctcagctggt cgaccctgaa gagatcgacg tcgtgcacgc cttctccggg
     1861 aatgatcact ctcccgtcac tgccgaggag gccggactga tgacgatgtg cgccgattac
     1921 ggtgtcgctt ccgtcaagtt cgtcagcggc gaatctgccc acgcacttca cgagcaagcc
     1981 gggaaacgaa gcgatctaat cattgtcggc tcccacggcc ggtccgggct gcggggcatc
     2041 attcactccc attccaacgc cacgctgcac gcaaccccct gcgatctgct gacggtgcac
     2101 ctggacgatc cgccccagag tcgctcgcca ccgtatcgaa gcgttctcgt cgcgattcac
     2161 ctgggcccgg tcacgcttcc ttccaaatgc gagcgatgca gtttttggcc gagcgcgcaa
     2221 gaggaggatc gactgaaccg aggccaccgg ttcatgaatg ccctcaaacc cataaagctg
     2281 atcccgcgcc attcacacgc cagcgaagag tcgcactacc ctctgtgcca cgttccaacc
     2341 gaggtaattc tgcacgcccg caagattgct cagcaaagcg gagccgagct ctctctgtgc
     2401 cacgtgtaca tgggctacga cgtcgatacc caacaaatgg aacgcgagct gctcgacagc
     2461 ctcggtgagc tctacgaggt tcccgaggat aggcggtact cggtgcccgg atcggtctca
     2521 ggtgcaattc accggctggc cagtcgcatc ggagcagatc tcgtcgtgct cggcacgcac
     2581 ggacgctcag gcccgagtct tgcggtgggt tcgaccgcca atgcggtcct gcacggcgca
     2641 acgtgcgatc agctgtcggt gcgaatcggc gcctgacacc cggcacgtcg gcgggcacct
     2701 cgtcaggccg ggctcagtcg gcgaacttgc gcacgttgat taaccggacg ataagacgat
     2761 aaacagcatt ccgttcgcaa cgtgcacgcc ggtgaaagag ctccgacgca gcgcttggcc
     2821 catccgcgcg cgggcgtgat actttcagcg tatggaaccg tcaggaagag cccatgaccg
     2881 aactcaccgc ggacctcatc gccgagcttc agcgtctgga tacgcccacc gtctgcaacg
     2941 cgctggaggt ggttgccccg gagcggcgcg gctacggcta caccgttcgc ccccttgtct
     3001 gcacccgccc ggagctgccg cccctggtgg cggtggcgcg cacggccacc atccgtgccg
     3061 cgcaccccgg acacctgcgc ggcagcgatg cacaggcact gcgtgacgcc tactacgcct
     3121 acgtggacga ggggcccaag cccagcgtca tggtcatcca ggacatggac ggcgaccagc
     3181 gcggctacgg ctcctggtgg ggcgaggtga acagcaacgt gcacaagggg ttcggctgcc
     3241 tcggcgtgat caccgatggc agcgtgcgcg acatcccgga catcgccgaa ggttttcaga
     3301 tgctggccga ccgcatcggc ccgtcacacg cctacgttca cccggtggac ttcggccgca
     3361 cggtgaacgt ggccggcatg cgcgtgaacg gcggcgacct gattcacgcc gaccagcacg
     3421 gcgcggtggt gatcccctgg gaagtcgcag ccgaggtcgc cgcggcggcg gacaccatcg
     3481 cgcgccgcga gcgggtcatc atcgaggcag cccgacagcc cggcttcgac atggcgagcc
     3541 tgcgcgacgc ctggggggac gcggcggaca ttcactagaa ttacccgggc gcgtcacgcg
     3601 ccggagagct cctgcacgat tcgccgatga gtcgcggcgt ccaggcggta ggcccgggcg
     3661 gcgatgatca gcaccagcat gatgagcgcc ggcgccaggg tcatcatgtt gtgaatggct
     3721 ctgagcgacg cctcggactg ctccacgttg gccacgtagc cggccagggc cagagctccc
     3781 gcgacgcctg ctccgcccac ggccttggcc agcttctgaa agaagctcgc gaaaccgaag
     3841 atggctccat cggctctgag ccccatgcgc cattgggcgt actccaccgt gtccgggatc
     3901 atggcccagc ccagcacggc gatgggcgtc ccgccaaggg cgatgacgca cgaccagacg
     3961 aacacccagg tcaccgagtc gtagggcgtc aggtacagac ccgcgcatcc gacgatagtg
     4021 acaagcgccc ccacgacgat gccgcccgct ttgccaacgg cggccgccag ccggggcacc
     4081 ccgagcaggc cgaccatcat cacgggaaga gtgatgccga agaaaagcgc gatgagatcc
     4141 tcccggccga ggttgtactt gaagtagtag agggtgaccg actggcggat ggtgaaagag
     4201 agcatgccgc cggtgaaaag cacgatcacg acgagcagcg gcgggtttgc gaacacggcc
     4261 ctgaagctgt cacgaaccgt cagcttctgg gtagccggcg gctgcacgcg ctcgcgcgtg
     4321 ttcgccacgg tcagccacag cagccccgtg gcaaacgtag cgaagatcac catggcccag
     4381 gcaaaacccg cggcttgggt ctcgaaaaga cccaccaggg gcagcatgcc cacggagacc
     4441 acgtagccac cagcgaacgc gcaacccatg cgcacggtgg tcagcagcgt ccgctcgtga
     4501 tgatcggccg tgatcgacgc ggtcagcgcc gagtagggaa tggagaccac cgtgtaggcg
     4561 atgccgaaca gcacgtacgt gacggccgcc caccagagct tgccggcatc gttcaggtcc
     4621 ggggtggtga aggtgagcac ggcgatcgcc cccagaggca gcgcgccgaa gagaatgtgg
     4681 ggccgaagcc gcccccagcg actgctcgtg cgctcggcaa tcccgcccat gagcgggtcg
     4741 gtcaccgcgt ccaccacccg ggccacgagg aacacgcctc cggcggccgc tgcgctcagc
     4801 ccgaacacgt ccgtgtagaa gtacagcagg tacgtcatgg ccgggatgaa gaacagattg
     4861 acgccgaaat cgcccacgcc gtagcccatg cgctcccgaa ccgaaagcgg ttggacgttc
     4921 acggcggctt caggccgatg ccgggtcggt gcaggcgatg gcgcccgccg ccgccagcgc
     4981 gtcgatgcgg gagctgggaa taccgagctc ggtcagcacg gcgcgggtgt caaaaccggg
     5041 gtccggagcc gcgttgcgga aggccgactc ctgaggaaag cgcgacaggt tgatggggga
     5101 gcggatgagg ttcaccgaac ccatatcagg gtgcggcgcc ggcttcgcca tgcccaggtg
     5161 gcgcacctga ggatcctcga aaccttcgcc aatggtgttg atggacccgc acggcacgcc
     5221 cgcctcgttc aggacgccca ccagatcggc cgacgtgcgc tggcttgtga tggcgttgat
     5281 cgcctccttg agctggtcct tgcgcgcgat gcgatccgcc gtggtctggt aatcgggatg
     5341 ggccagcaga tcgtccgccc ccagcgcctc gcagaaccgc tgccacaggc gcggcatgga
     5401 ggcagcgatg ttcacctggc cgtcgctggc caggaacgtg cccatggggg tgtaggccgg
     5461 gtggtcgttg ccctgctggg tcgggacctc gccgttcatg gtgtagcgcg cgccctgaaa
     5521 atccagcttg cacatcatgg cttcgagcag cgacgtgtga acccactgcc cctcgcccgt
     5581 ccgctcccga tgcagcagcg ccagcaggat gccctgccct agaaacatgc cggcggacgt
     5641 gtcgctgatg gccacgccca cccgcatggg gccgcctccc ggctcccccg tcaccgacat
     5701 gagcccgctc atgccctgga cgatctgatc cacgccgccc cggtccgcgt aagggccctc
     5761 ctgaccgaag cccgagatgc tcgcgtagat gatgctttcg ttaacctgct tgagcgtctc
     5821 gtagtccacc ctcaggcggt gcttgacgtc caccttgaag ttctcgacca ccacgtcggc
     5881 cacggctgcg agctcgagaa acagcccgtg gccatccggg tgcttgagat cgagacagag
     5941 gctgcgcttg ttgcggtgca ggttctgctc gtccggcccg cgcctggccc cggtcaccga
     6001 ttgccctgcg cccggcacag gctcgatctt gatcacgtcg gcgccccagt ccgccagcag
     6061 ccgtacggca ctcgggcccg cgcgggcgat ggtgaggtcg agcacgcgaa tgtgggaaag
     6121 cggccccatc tcagcgaatc agccggccgg gcagcgcctc cgtggcctcg ccgttctcgt
     6181 aggtgacctg gccgctgacg atggtggcca gataaccgtc cgccttctgc tgcaggcggg
     6241 caccgccggc ggggaggtcg tgaacgatct ccggcgcgcg caccttcagg cgctcgaagt
     6301 cgatgacgtt gatgtcggcc ttcatgccgg gtgcgaggac gccgcggtcg ttgaggtcca
     6361 cagcccgggc cgtatccgcc gtctggcttt tcaccagcac gggtagaggg atgcgctcgc
     6421 cccgggtgcg gtcccggccc cagtgcgtga gcagggttgt cacgaagctg gcatcgcaga
     6481 tcgtgcccac gtgggcgccg ccgtcaccca ggcccatgac ggtatccggg tgcaggatca
     6541 tctcccggca gcagtccagg ttgtcctcgg cgtagttggc gaacggcgtg tagagaatct
     6601 gccgcccttc ggcttgcaga agctggtcgt agacgtactc ccaggggtcc acccgggcca
     6661 cccgggcgcg gctcgccagg ctgtcctgcg gcgccggctc gtagtcgggc gggtcctcta
     6721 aacgccagat cttggcgccg ttggccatga gcccccacag gcgctggaat ccgcgccccg
     6781 gggatcccgc gaggatggcg gccctgcgtt ccgggtcgcg catggcgcgc acccgctcgg
     6841 ccagcgccag gccggcgatc tcccggtagg ccgggtgggt gacgaacggg ttcagcgtgg
     6901 tggtgagccc caggagaatg ccgatgggcc ggggggcgac ctgcgcgcgc atgggcaggc
     6961 ctgcttcgct ggcgctggca atgcggccga gcaggctgcg ccagccgttg gggctcaagc
     7021 cctgagccag cgagatggac atgggtcggc cgctctcccg cgtcatcgcc gtcagatgag
     7081 cgaactcctc gtcggcatcc tggaagtcgg aaatcatctc catgacgccg cggcccgcgt
     7141 ccttgagccc gcgggcgatg ccgagcagtt cctgcctggc cgcggtgagc gaaggcgtcg
     7201 gtgcgccctt gctgctgcgg tggttgagcg tgcgcgagct ggaaaaaccc agcgcgccgg
     7261 cctcgatggc ttccgccgtg agcgcgcgca tcagggcgat gtcgtctgcg ctggctgcct
     7321 cccggtccgc gcctcgccga cccatgacgt acacccgcaa ggcgccgtgg ggcagctgtg
     7381 cgccgatgtc catgtcgaac gagcgctgcg atagatagtc gaggtactgg ggaaaggtct
     7441 cccactccca gtggatgcct tcgtgcagcg cagcgccagg gatgtcctcc acgccttcca
     7501 tgagctcgat gagcaggtcc cggtcggtgg gtttgacggg cgcgaacccc acgccgcagt
     7561 tgcccatcac caccgtggtg accccgtgat ggctcgaggg ggacaggcgg ttggaccaag
     7621 tggcctggcc gtcgtagtga gtgtgaatgt ccacgaagcc gggcgtgacc agggcgccgt
     7681 cggcgcggat ctcgcgccgg cccgagccgt cgacctcacc caccgccacg atgaccccgg
     7741 cacgcaccgc cacgtcggcc gtccgttccg ggctgccggt gccgtcgacc accgtgccgc
     7801 cgcgaatcac cagctcgtgc tcgctcatct ccacgtctcc cggacatttc ccccgaccgc
     7861 cggattctcg cacatcgccc cgtcttgtct acaatgcgcg ctttcgcgac taagagcccg
     7921 ccgttgcagc gcgtcaccat catcctaatc gtcctgtcgg ggctcatcct tgccctgttt
     7981 gcctggtttc cggcggaaac cctgctggac gccgcgagag cgctggaccg cacgccgccc
     8041 gagccggcga cgccggacga cgtgacccgg cggaccaccg aaagcgggga cgtcgtcggg
     8101 ttcatcggca accacggcgc gcgcacgtgg cgcggcgttc cctatgcccg cccgcccgtg
     8161 ggcgcgctgc gctggcgtgc gccggagccc gccgagccgt ggcccggcgt tcgcgaggcc
     8221 accgctgccg gaccgctgtg cgcgcagaga gcgggggtga tctccggcga cgggttcgcg
     8281 cccacccggg tggcggcggg cagcgaggac tgtctgtatc tggacatcta cgccccggcc
     8341 aacgcccggc gcctgccggt gatgttctgg atacacggcg gcggcaactc cgtggggcac
     8401 aacggcccct acgacggcgc ccgtctggcc acctggcgcg acgtggtggt ggtcacggtg
     8461 aattaccggc tcgcccacct cggctggttc gcccaccccg ccctgaccac cggcaaccgg
     8521 ctgcacgact cgggcaacta cggcaccctg gacctcatcc aggcgctgac ctgggtccgc
     8581 gacaacatcc aggcgttcgg cggcgacccg gacaacgtca ccgtcttcgg cgagtccgcc
     8641 ggcggcacca acacgctcag catgctggcc tcgcccaggg cggccggcct gtttcaccgg
     8701 gccatcgccc agagcggcgg gctcacgccc atgcccatgg cccgggcaag cgcatacgcc
     8761 gaggacggcg gccaccccag cagcgctcgt gagatcgtca accgcctgct gatcgccgac
     8821 ggccgcgcaa gctc
//
LOCUS       DBNHLM010000291        22297 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_581455, whole genome shotgun sequence.
ACCESSION   DBNHLM010000291 DBNHLM010000000
VERSION     DBNHLM010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 22297)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 22297)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22297
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_581455"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..973
                     /locus_tag="AC16QF_13475"
     CDS             167..973
                     /locus_tag="AC16QF_13475"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6449618.1"
                     /translation="MADLKGKVAVVTGASRGVGRGVAEGLAEAGATVYVTGRSESHDN
                     PPQERTIQATAASVDALGGKGIAVRVDHNEDAEVKALFERVMSEQGRLDVLVNNVYKI
                     PDPPVFDGGFWEHPVSVWDDQCGVGLRGYYVASVFAAPMMVARRSGLIVNISSRGGDQ
                     YVFSTSYGVGKAGVDRMARDMAHELRGYHVAAVSLRPSSVKTEFILDAVASGAVKLDM
                     AKAQSPRFTGRCVAALAMDPDLMEKSGGVYMVADLAAEYRFTDPDRVEAS"
     gene            975..1697
                     /locus_tag="AC16QF_13480"
     CDS             975..1697
                     /locus_tag="AC16QF_13480"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6449619.1"
                     /translation="MQPVERHVDVATPDGSMNTFIAYPQGGGPFPVVVFYMDAPGKRE
                     ELHDMARRLASAGYYVMLPNLYYRRVREFVMTAEARPEMMAHMNSLSNHMACEDTAAL
                     FAFASADRAARDGPAGCVGYCMSGPFAFAAAAAFPDRIAAAASIHGVRLCTDAIDSPH
                     LNAEHVGGELYFACAEHDDWAPPEMIEALEGHLSRVGASYRIEWYPGTSHGFVFPARP
                     VYHPAAAERHWERVLDLFSRNL"
     gene            complement(1681..2751)
                     /locus_tag="AC16QF_13485"
     CDS             complement(1681..2751)
                     /locus_tag="AC16QF_13485"
                     /inference="COORDINATES: protein motif:HMM:NF014653.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HIR6449620.1"
                     /translation="MLIDRDALTRFVSEIFVRLDAEASDAQEVAAHLVSANLKGHDSH
                     GVGMVPTYVGNIRSGALALNTHARLVRDKGAVMLIDGQMGFGQVVGREAVELAIERVR
                     QTGVVAAGVRNCHHLGRIGHYGEICGRAGYVSMHFVNVVGHQPLVSPFGGRQARISTN
                     PFCCVVPRSDEPPVVLDMATSAIAQGKVRVAYMKGDKVPDGSLIDHEGKPTNDPSVMF
                     EDPRGSLGPFGHHKGYGLALMCELLGGGLAGEWTAQAPPPDRFTIVNHMLMVVLDPDL
                     FGGAEAFHREVEEMIAYLRSTMPAAGVDRVRVPGEPELESMAERAAGGIPIDDNTWAA
                     IRKSAQVSGMTEEEIAGFTGSG"
     gene            2832..3689
                     /locus_tag="AC16QF_13490"
     CDS             2832..3689
                     /locus_tag="AC16QF_13490"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR6449621.1"
                     /translation="MDKCAEGFSLIEGPVWDPDRGLLFSDVLVGGVFCLGGDGTVTTV
                     FEHRRGIGGMALHEAGGLIVSGRNLSFKPYDEGSTVTVLDRDEENGLVGFNDLTTDAE
                     GRIYAGSLGASPVFADGREPRAGDLYLVDLDGSARVVAEDVRLTNGLGFSPDGTTLYH
                     SDSGRRTVFRYDVQPDGSLSAKEPFVTPDNGVPDGLAVSRDGAVWVALARGGHGVAVY
                     EASGRLREFIEIPLPMCTSVCFGGEDLKDLYIVSGSAGAGSDRAGAVFRERVDVAGLI
                     MTPARVRLA"
     gene            3757..4587
                     /locus_tag="AC16QF_13495"
     CDS             3757..4587
                     /locus_tag="AC16QF_13495"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6449622.1"
                     /translation="MAIEPMDTGTEDLLAHQEDGVAVITLNRPQARNAMSAAMNAGLA
                     KALDEAERNPAVRCVVITGAGGAFCAGGDVKGMAARSQGGGPGPTVDERIHLQRLNQR
                     ATAGRIYQMPKPVLASLPGPAAGAGLALALAADLRIMADSAFITTAFAKVGFSGDFGG
                     SFFLSRLVGSGKAKELYFLSDRVSAAECERLGIANAVVPAEELEAQTMALARRLASGP
                     PVAYRYMKENFNRAAAGAGVLECLDLEATHHVHTGLTEDHRAAAKAFVEKTEVEFKGR
                     "
     gene            complement(4625..5602)
                     /locus_tag="AC16QF_13500"
     CDS             complement(4625..5602)
                     /locus_tag="AC16QF_13500"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIR6449623.1"
                     /translation="MDIKDGFAGTIGNTPLIRMNRLSEETGCEILGKAEFLNPGGSVK
                     DRAALWMVREHERSGALKPGGVVVEGTAGNTGIGLVHVCNARGYRSIIYMPDTQSPEK
                     VQLLKTLGAEVREVPAVPYRDENNYQKQAGRCAETLENGVWANQFDNVANRLAHYEST
                     GPEIWDQTEGRVDAFTCATGTGGTLAGVSRFLKERREDVRIVLADPMGSALYNWVTTG
                     ETTMSQGPSITEGIGNSRVTDNLAGTCIDDAVQVTDRDAVNMVYRELREEGWFFGSST
                     GINLCAAVQVAHSMGPGHTIVTVLCDTGAKYQSRLFNPEWLAERGLTPG"
     gene            5693..6859
                     /locus_tag="AC16QF_13505"
     CDS             5693..6859
                     /locus_tag="AC16QF_13505"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6449624.1"
                     /translation="MKKADLQDRWLGDAGENGVNPLRIAALMARVRKEVDEGLLPAAQ
                     VALARNGALVLFETMGAADNDSLFCVFSATKAITSAAAWILMQEGRLKEDELVADIIP
                     EFAANHKHDITVGQLFVHTAGFPSAPFKPLDWNDRARRLERFAQWQLNWPPGSRYEYH
                     PTSSMWVIAEIIERRANTTYQEFVRERIALPLGLPELYVGLPATENERVVPNVHVGDA
                     LTRADYELLGLPEPPVTEVTEEAILKFNRPEVRAVGVPGGGGIMSAADLALFYQGLLH
                     GGLGEEVWSAATLADARRVRSGELRDPVMGHRANRALGLVIAGDPQRNLRGFGKTNSP
                     ETFGHNGAGGQLAWADPATGLSLAYCTAGHDRNRIRQGRRGVAISSLAAVCCED"
     gene            complement(7382..7777)
                     /locus_tag="AC16QF_13510"
     CDS             complement(7382..7777)
                     /locus_tag="AC16QF_13510"
                     /inference="COORDINATES: protein motif:HMM:NF039797.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HIR6449625.1"
                     /translation="MPSTSKFAKLACSIAGTVLLYASSGLALPPDSPIGPRAESIVEG
                     TVQAPVSSIPAANRIEGWARVDDKRVVLEVSPQTRYLLTLRQGCIGLGFARNVSVTMS
                     NNTIYAGFDAITADGEHCEIRTINPLVTP"
     gene            8065..9435
                     /locus_tag="AC16QF_13515"
     CDS             8065..9435
                     /locus_tag="AC16QF_13515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6449626.1"
                     /translation="MAASAESVLERVDAEAAAAVSMAERLWALAELGYQETRSSELLG
                     AYLAESGFSLESGVAGIPTAFVATFGSGRPVIGVLAEFDALPGMSQAAEPSRRPLEPG
                     GAGHACGHHLFGAGAAAAAVSVARWMEANGVPGSIRVYGTPAEEGGSGKVYLTRAGLF
                     DDVDVVLHWHPGSRNDASPATTTANKSGRFTFRGVAAHAAAAPERGRSALDGVESMNY
                     MVNLMREHVPSDSRIHYVITKGGDAPNIVPEIAQVYYYVRHPEAATVIELFERVVAAA
                     RGAAMGTETDVDYEVMHGNYAVLPNETLAKRVHAHLLSLGGVSYSPDERRFAEQIQAT
                     LGDSLPPLSRAASVEPFQMRQNMGSTDVGDVSWNVPTVGFYAATWVPGTPAHSWQAVA
                     AGGTSIGHKGMLLATRTLALTAADLLRTPSLIAAARAELEERRGQDFRYVALLGERDP
                     PLDYRR"
     gene            9432..10274
                     /locus_tag="AC16QF_13520"
     CDS             9432..10274
                     /locus_tag="AC16QF_13520"
                     /inference="COORDINATES: protein motif:HMM:NF020694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6449627.1"
                     /translation="MNLGVLHPGEMGVSVGAVLTAAGHRVYWLPESRSRATRERAEGA
                     GFTGCASLRELADRVAGIVSVCPPAAAEEVASQVRSAGFTGVYVDANAVSPATARRMA
                     RTMGPTYVDGGIVGPPAVRPGLARLYLSGAAAEEVAGWFHGGLLEARVLAGEAGAASA
                     LKMCYAAYTKGSSALVLAIRALAEAEGVDEPLLAEWDISQPGLADRSQRAAAGTAAKA
                     WRFVGEMEEIAATFRGVGLPGEFHEAAAVVYRRLAPLKGNAAAELADVVRRLIENPAD
                     VGED"
     gene            10258..11298
                     /locus_tag="AC16QF_13525"
     CDS             10258..11298
                     /locus_tag="AC16QF_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449628.1"
                     /translation="MSGKISRIARMAVSWAIAAPAIAAPGYLFDEAATLDLELEAPLG
                     ALMGDRYPDSEYRPAQLRYVGKDGSRVALPVEVRTRGKTRRDEEVCKMPPLRVRFDQA
                     AADGTPFAGQPALKLVTYCRDSDSFDQLVLLEYLAYRTYNLLTPQSHRVRLARISYLE
                     RGDRRTTRYGIFLEDWRAVAARNDLSADPVDGGVNVDKLNHDAANRVAVFAYLIGNED
                     YSLLWPEPDENCCHNIKPLLDRDGRVVPLPYDFDYSGLVNAPYAVAKNPRRKVRQRAY
                     RGLCDTQEGLEAALELTRARREDIYAVFRAQEGLSPAKLKSSMGYLDRFYDLIDQPAQ
                     VEKRLRRRCRKS"
     gene            complement(11303..11683)
                     /locus_tag="AC16QF_13530"
     CDS             complement(11303..11683)
                     /locus_tag="AC16QF_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449629.1"
                     /translation="MGYPATKLTVILIVSAIAAPAFANPVQCTQGSLVRSVEVVYSGP
                     DQTLPCEVIYDKTQEGAGIHSLWRASNEAGYCEAQAAAFADKLGAMGWDCGEPAGSGI
                     DVDSGDAPSAADVPAEIPIAPDPA"
     gene            complement(11700..13286)
                     /locus_tag="AC16QF_13535"
     CDS             complement(11700..13286)
                     /locus_tag="AC16QF_13535"
                     /inference="COORDINATES: protein motif:HMM:NF013225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR6449630.1"
                     /translation="MALRPPQPDADHMSWEEEIRELRKREQLAYQMGGAEKVARQHEF
                     NKLTIRERIAAISDSGSFQEIGTLAGVGQYDDDGNLIGFTPSNFVFGAAEIDGRPVIL
                     SGDDFTVRGGSADASIIGKRLQAEGLATELRLPHVRLVDGMGGGGSVKTIEMAGRTYI
                     PEVRGWETVVQHLAVAPSVSLALGSVAGIGAARVATSHYSVIVKNSAQMMIAGPALVD
                     WASLGDVSKEELGASRIHTRNGAIDDEASSEEEAFDMARRFLSYLPTSTDELPPVTDA
                     TDDPERTDEALLEIVPKDPRKVYKMHRIIDSVVDEGSFFEIGRRWGRSIITGLARLNG
                     MPVALFAENPMVYGGAWTADASRKLVRFIDLASTFHLPLVHLEDCPGFLIGKQSEESG
                     TIRFGCQALAALGQAPVPFCCVVVRKAFGVAGASNHKPGSHCVRMAWPSGDWGSLPIE
                     GGIEVAYKADLAQADDYDAHLSRIKERLNRLRSPFRSAEHFEIEQIIDPRETRPALCR
                     WMKLARQALRTDPPTFWFRP"
     gene            complement(13315..13611)
                     /locus_tag="AC16QF_13540"
     CDS             complement(13315..13611)
                     /locus_tag="AC16QF_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449631.1"
                     /translation="MSADGTWNTVMNTPMGAQNGTMTLATDGGTLTGTLKGPQGEIEL
                     KDGTADGDSLAWKADITTPMAMTLEFSATVNGDEMTGNVKLGAFGDATFTGTRA"
     gene            13707..14402
                     /locus_tag="AC16QF_13545"
     CDS             13707..14402
                     /locus_tag="AC16QF_13545"
                     /inference="COORDINATES: protein motif:HMM:NF053177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8091 domain-containing protein"
                     /protein_id="HIR6449632.1"
                     /translation="MSIGTLNEGPLHQAIKSLYNGAGAREEVPVGDFVADVFGPDEVI
                     YEIQTGGFGSLKKKLARLVEDHRVVLVHPIAEVRYIVKLPQGEDQQASRRRSPKRGSV
                     AHVLDRLVSIPALLNHPNFELEVLLIEEEELRTFDPRRVRRRDGWRVVQRRLGSVLAS
                     ERFTCAGDLLRMVPGPLPAEFTTEDLAGAMGQPRWLAQKLAYCLREAGEISISGKRGN
                     ALCYRLARSGAVA"
     gene            14458..15135
                     /locus_tag="AC16QF_13550"
     CDS             14458..15135
                     /locus_tag="AC16QF_13550"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6449633.1"
                     /translation="MIELYTASTPNGWKASVTLEELGLPYEVRPVDLMAGEQKAPEFL
                     KINPNGRVPAIVDDGFPIFESGAIMIHLAEKTGRLMPADAHGRSLVIQWLMFQMGGVG
                     PMMGQANVFYRAMEEKIQPAIDRYQNECRRLFEVLDGRLRNSEWLADHFSIADIANWC
                     WARLYYWSGVSLDGLDAMQRWMAAMEARPACQRGVEVPNRLELPRDDDPDGIERQALQ
                     SRRVLVR"
     gene            15135..16952
                     /locus_tag="AC16QF_13555"
     CDS             15135..16952
                     /locus_tag="AC16QF_13555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR6449634.1"
                     /translation="MALESVIEGPAWDLTGEYSSPDSQGLQADLDRLSELLDRIEALN
                     GQLNGSGAVAAAQQIHRLADQARELLADPRAYASCLLSVNGRDEAALMLQGRLQGYQT
                     RFGDLLKPLELFIDLADDEGIEAFLADPEVAPAEFVVRHARKRRHERLGLAEEQLING
                     LSQEGIHAWGNLYDQLSGTLTCEVLVGNETREMGIAEAAGLLQKPDVATRRNAWRAIN
                     DSWSAHAETCAASLNAIAGWRLELGRRRGREAPVHFLDAPVHMNRISRETLDTLMDVA
                     AGSRPLARRAARLMARAYGLETIGPWDQRAPAPDLGTAPAAGIPYDQALHTIADAYAD
                     VHPSMGEFVRMMHANRWIEGTRGPNKRPGAYCTGFAKSRTPRIYMTYSGGSSDVITLA
                     HELGHALHSWVMRDLPNSQRGYGMALAETASTFGETLVRDALLGSAGSPQAKLDMAWE
                     EMAALVSFVLNIPTRFEFERRFYEARRERPVLPRELNAMMADAWTECFGDVLEEPDPM
                     FWASKLHFYISGISFYNFPYLFGYLFSLGVYARRGDGADFYPRYEGLLRDTGRMTAEA
                     LAAKHLSEDLSRPGFWEATVASLETRVDAFEDLLDELAL"
     gene            complement(16949..19276)
                     /gene="lon"
                     /locus_tag="AC16QF_13560"
     CDS             complement(16949..19276)
                     /gene="lon"
                     /locus_tag="AC16QF_13560"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR6449635.1"
                     /translation="MPVLPVLPLKDTVVYPQIVRPLGVGRRRSLAALNHAVEGPREII
                     TVAQHDGANEEPGLTDLYAVGCIASISRVENRDGGAQVIVQGTRRVRLTGIAEDEGAF
                     LAAQYEELPGLGMNDLGTDGPAVDATLRENLQLAQRIAEIMDSENGAQIYQQLVGSIS
                     DPITQMYRIASLAPGIELIQEQRILEADTTLALMEAIHELLGHELQVTELRRQIAERA
                     RDDMDQQQREHVLRQQKRAIEQALGESGEDDDIAELKGQLDEARLPEPVQAEVDRELK
                     RLARMSANAADYQVARAYLELIGELPWHQETEDQLDLKHAREILDEDHYGLEDVKERI
                     LESLAVMQLNPGAKAPILCFVGPPGVGKTSLGQSIARAMGRKFERLSLGGLHDEAELR
                     GHRRTYIGAMPGRILQGVRRAGVTNPILMLDEVDKLGRDFRGDPAAALMEILDPAQNR
                     EFRDNYLNLPYDLSRVLFITTANTLEGIPRPLLDRMEVLDLTGYSDLEKQAIARQYLI
                     PRQRSEAGLSEAQLDISDDALTTMIRRYTREAGVRGLERVIGRLARKRARQVVDGQSL
                     DTPVAADDLAELLGPERFKSDRGRTGLEPGVAAGLAWTEAGGDVLYVEATLTQKDEKV
                     TLTGHLGQVMQESARAARSHLWSAADRLGLDRPAIEESGVHIHVPAGAVPKDGPSAGV
                     TMACALYSAYKGRALRDDVAMTGELTLSGLVLPVGGVKEKILAAHRAGLRRVILPKDN
                     EADLAKLPQTVLDEMQISLAAHLDDVLAVAFAEES"
     gene            complement(19287..20354)
                     /locus_tag="AC16QF_13565"
     CDS             complement(19287..20354)
                     /locus_tag="AC16QF_13565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449636.1"
                     /translation="MEFGLSEEQTLLQDSLNGFLDANCPLDRVRRFAEEPVAADLHSG
                     LAELGVFGLLVPEEAGGVGLGCFEAALVAEALGSHTAPCAFLGSAVMAPLALTLAGNE
                     PTDTLSRLATGELTVGVAVAEFVGARADGGITADGETLSGKALSVLDFDAQAYLVADA
                     AGSTYLVDAGAPGVFAEALPTIDKTRRTGALTLNGAPAQLICDDPDVLARVIDAGRVM
                     LAADTLGAAQNMLDQAVEYAMQREQFNRPIGSFQAVKHMCAEMAASLEPCRAMVWYAS
                     HALDDLPAEASLCACHAKAHLSEVGKFVAKTATEVHGGMGFTDLLGLHYWFKRIGYNR
                     QMLGAPELLREQAARLQGLCA"
     gene            complement(20364..21554)
                     /locus_tag="AC16QF_13570"
     CDS             complement(20364..21554)
                     /locus_tag="AC16QF_13570"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449637.1"
                     /translation="MDLSYGPHYEEFREEVRSFLADHAGSAPRGEHIRSEKTLAWQHL
                     LIENGYAARTIPREYGGYGAAPDILESRIIAEEFAAARVSSGLGGQGISMLVPTLLEM
                     GTQEQKTTFIPPTLRGEMLWCQGYSEPGAGSDLASLSTRAELDGDEWVINGQKIWTST
                     AHIADWMFCLVRTEPDAPKHQGISFLLLPMDTPGIEVRPLVDMTGAANFNETFFTDVR
                     VPKDQIVGRRGEGWLVANAILGHERDSLGDPNVMLSRFNALTELMKTETLGGERLIDH
                     PVFRDRLMQIQGRVMALSYNDLRLLSAKVNAADARLARMIVKLQGTELRHDLEGLAVD
                     AMGELGLAYGDNPYLRDHGSWQSQYMFYLGLIIGGGTSQIQKNIISERGLGMPKEPKY
                     EKQA"
     gene            complement(21654..>22297)
                     /locus_tag="AC16QF_13575"
     CDS             complement(21654..>22297)
                     /locus_tag="AC16QF_13575"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6449638.1"
                     /translation="ANKRVFQLNRVYLEHREHRPPWTKEAPDVGNYHKSVGIVGASHV
                     GRLVMEHLRRFDIRLLLYDPFVTPLAARDLGATKVGLSELLSQSDTVSLHAPLLKETR
                     HMIGARELALMHDGATIVNTARGALIDQAALEAELTRGRLYAVLDTTDPEELPPESPL
                     YGLPNVFLTPHIAGSLGTETQRLTDCIIEELERYGRGVPLKYRVRREQLDRLA"
BASE COUNT         3558 a         7269 c         7541 g         3929 t
ORIGIN      
        1 ttcaggccgg cacgtttgat gtcggatccg ggcaacggct ttccccaacg gtagttgggg
       61 acactagccc atgcagggct gaccgtccac ccgtgaccat ggggcgtgga gtggctgggt
      121 tacaatcgcc gtcggcacgg ggtaacgaca tcgggaggac gatcacgtgg cagatctgaa
      181 aggcaaggtg gcagtggtta ccggtgcgag ccgcggggta ggccggggcg tagccgaagg
      241 cctggccgag gccggcgcca ccgtctacgt caccgggcgc agcgagagcc acgacaaccc
      301 gcctcaggag cgcaccattc aggcgaccgc ggcgagcgtc gatgcccttg gcggcaaggg
      361 aattgccgtg cgggtagatc acaacgaaga cgccgaggtc aaagctctct tcgagcgcgt
      421 catgagcgag cagggcaggc tcgacgtcct ggtcaacaac gtctacaaga ttcctgatcc
      481 gccggttttc gatggcggct tctgggagca cccggtatcc gtctgggacg atcagtgcgg
      541 ggtggggctt cgaggctact acgtggcctc cgtgttcgcc gcgccgatga tggtcgcccg
      601 gcgcagcggt ctcatcgtga acatctcctc acggggcggc gaccagtacg tgttcagcac
      661 ctcctacggc gtgggcaagg cgggggtcga tcgcatggct cgggacatgg cccacgagct
      721 gcgcggctac catgtcgctg ccgtgtccct gcgaccctcc tcggtgaaga ccgaattcat
      781 tctggacgcg gtggccagcg gcgcggtcaa gctcgacatg gccaaggcgc agtcgccgcg
      841 gttcaccggg cggtgcgtgg ccgcgctggc catggacccg gatctcatgg agaagtcggg
      901 cggcgtctac atggtggccg acctcgcggc cgagtaccgg ttcaccgacc cggaccgcgt
      961 ggaggcgagc tgaggtgcag ccggtggaac gtcacgtgga cgttgccacg cccgacgggt
     1021 ccatgaacac gttcatcgcg tacccccagg ggggcggtcc gttccccgtg gtggtgttct
     1081 acatggacgc gccgggcaag cgcgaggagc tgcacgacat ggccagacgg ctggcgtcgg
     1141 ccggctacta cgtcatgctg ccgaacctct actaccggcg cgtccgggag tttgtcatga
     1201 ccgccgaagc ccggccggag atgatggccc acatgaactc gctttcaaac cacatggcct
     1261 gcgaggacac cgccgctctg tttgccttcg catcggcaga ccgagcggcg cgggatggcc
     1321 cggccggctg cgtgggctac tgcatgagcg gacccttcgc ctttgccgcg gctgccgcct
     1381 tccccgatcg cattgccgcg gccgcctcca ttcacggcgt gaggctttgc acggacgcga
     1441 tcgattctcc ccatctgaac gcggagcacg ttgggggcga gctttacttc gcctgcgccg
     1501 agcacgacga ctgggctccc ccggagatga tcgaagccct ggaggggcac ctttcccggg
     1561 tgggcgccag ctatcggatc gagtggtatc cgggcacttc tcatggcttc gtcttccccg
     1621 cacggcccgt ctaccacccg gcggcagccg agcggcattg ggaaagggtt ctcgatctct
     1681 tcagccggaa cctgtgaacc cggcgatttc ctcttccgtc atgccggaca cctgggccga
     1741 tttccggatc gcggcccagg tgttgtcgtc gatgggtatg cccccggccg cgcgctcggc
     1801 catggactcg agctcgggtt ccccgggcac ccgcaccctg tcgacgccgg cagccggcat
     1861 cgtgctgcgc aggtaggcaa tcatctcctc tacctcccga tggaaggcct ccgcgccgcc
     1921 gaacaggtcc gggtccagca cgaccatgag catgtggttg acgatggtga accggtccgg
     1981 aggaggtgcc tgggccgtcc actctccggc gagcccgcca ccgagaagct cgcacatcag
     2041 ggcgagcccg taacccttgt gatgtccgaa tggccccagc gagccccggg ggtcttcgaa
     2101 catcacgctg ggatcgttgg tcggttttcc ttcgtggtcg atcagggatc cgtccggcac
     2161 cttgtctcct ttcatgtagg ccacccggac cttgccctga gcaatggcgc tggttgccat
     2221 gtcgagcacc acagggggct cgtcgctcct cggcaccacg cagcagaaag ggttggtgct
     2281 gatgcgcgcc tggcgcccgc cgaacggcga gaccagcggt tggtgtccca ccacgttgac
     2341 gaagtgcatc gacacgtagc ccgctcgccc gcagatctcg ccgtagtgcc cgatgcggcc
     2401 caggtggtgg cagttgcgca cgccggccgc gacgacgccc gtctgcctga cccgctcgat
     2461 ggcgagctcg acggcttcgc gccccaccac ctggccgaaa cccatctgcc cgtcgatgag
     2521 cataacggcg cccttgtccc ttaccagcct ggcatgggtg ttcagcgcga gagcgcctga
     2581 gcggatattg ccgacgtagg tcggcaccat gccgacccca tgggaatcgt ggcctttgag
     2641 attggcggac accagatggg cggcgacctc ctgcgcgtcg ctggcttcgg cgtcgagccg
     2701 aacgaagatt tcgctgacga agcgcgtcag tgcatcgcgg tctatgagca tggtggcttc
     2761 tcatcgtgtt ggaacgctca gtgttgcctc tttaaagagc ctgtgacaag gtctgacgag
     2821 agggggttgg catggacaag tgcgcggaag ggttcagcct gatcgaaggg ccggtctggg
     2881 atccggaccg cggcctgctt ttcagcgacg tcctggtcgg tggggtcttc tgcctcggcg
     2941 gcgacggcac ggtgacgacg gttttcgagc atcgtcgggg catcggcggc atggccctgc
     3001 acgaagccgg tggcctgatc gtcagcggcc ggaatctgtc tttcaagccc tacgatgagg
     3061 gctcgacggt caccgttctc gatcgcgacg aggagaacgg cctggtgggc ttcaacgatc
     3121 tcaccaccga tgccgaaggg cgaatctacg ccggcagtct gggagcgagc cccgtgttcg
     3181 ccgacgggcg tgagccgcgt gcgggggatc tctacctggt cgatctggac ggcagcgccc
     3241 gggtcgtggc cgaggacgtt cggctgacca acgggctggg gttctctccg gacggtacca
     3301 cgctctacca ctccgacagc gggcgccgaa cggtcttccg ctacgacgtg cagccggacg
     3361 gttcgctctc ggcaaaggag ccgttcgtta cgccggataa cggggtgccc gacgggctcg
     3421 cggtatcccg cgacggcgct gtctgggtgg cgcttgcccg gggcggtcac ggcgtagccg
     3481 tctatgaggc gtccggccga ctgcgtgagt tcatcgagat tcccctgccc atgtgcacca
     3541 gcgtgtgctt tggcggcgaa gatctgaagg acctttacat cgtctccggg tccgcgggcg
     3601 ccgggtctga cagggcggga gccgtcttcc gagagcgtgt cgatgtggcc ggcctgatca
     3661 tgacaccggc tcgggtgcgg ctcgcgtgac gaaggcgtcg ccgtcgacct gcaggcaggt
     3721 gcgtcttcat cggttagagc agaggagttt gcaggcatgg cgatagaacc catggacacg
     3781 ggaaccgagg atctcctcgc acatcaggaa gatggcgtcg cggtgatcac gttgaaccgg
     3841 ccccaggcac gcaatgccat gtcggccgcg atgaacgcag gcctggcaaa ggctctggac
     3901 gaggccgagc ggaatcccgc cgtgcgctgt gttgtcatca ccggagccgg cggcgcgttc
     3961 tgcgccggcg gagacgtgaa aggcatggcg gcgcgaagcc aggggggcgg ccccgggccc
     4021 accgtggacg agcgcattca ccttcagcgc ctgaaccaga gggcgacggc cggccgcatt
     4081 taccagatgc cgaagccggt tttggcgagc cttcccggac cggctgcagg agcggggctg
     4141 gctctcgccc tggcagccga tctgcgcatc atggccgact ccgccttcat cacgacggcc
     4201 ttcgcgaagg tgggcttcag cggcgacttc ggtggcagct tcttcctctc tcgcctggtt
     4261 ggcagcggga aagccaagga actctacttt ctttccgatc gggtgagtgc cgctgagtgc
     4321 gagcggctgg gaatcgccaa cgcggtggtg ccggcggaag aactcgaggc ccagacgatg
     4381 gcgcttgcgc ggcggctggc gtccggaccg cccgtggcat accggtacat gaaggagaac
     4441 ttcaaccggg ccgccgcagg tgccggggtg ctcgagtgcc tcgacctcga ggccacgcat
     4501 cacgtgcaca ccggtctcac ggaggatcat cgcgcggcgg caaaggcctt cgtcgagaaa
     4561 accgaggtcg agttcaaggg ccgatgaggc gttcctccag gagccgcggc gctcgggcgc
     4621 gtcctcagcc cggtgtcaga ccccgttcgg ccagccactc ggggttgaac agtcgggact
     4681 ggtacttggc accggtgtcg caaagcacgg tgacgatcgt gtgtcctggc cccatcgagt
     4741 gggcaacctg aacggcggcg cacaggttga tccccgtgct cgaaccgaaa aaccaaccct
     4801 cctcccgcag ctcgcggtag accatgttca cggcgtcccg gtcggtcacc tgaaccgcgt
     4861 cgtctatgca ggtaccggcc aggttgtccg ttacccggct gttgccgatc ccttcggtga
     4921 tggacgggcc ctggctcatg gtggtctcgc cggtggttac ccagttgtag agggcgctgc
     4981 ccatggggtc ggcgagaacg atgcgaacat cctcgcgtcg ttccttgaga aagcggctca
     5041 cccctgccag cgtcccccca gtcccggtgg cgcaggtgaa agcgtccacc cgtccctcgg
     5101 tctggtccca gatctccggc cccgtggact cgtagtgcgc caggcggttg gcgacgttgt
     5161 cgaactggtt ggcccacacc ccgttttcca gggtctcggc gcagcgtccg gcctgtttct
     5221 ggtagttgtt ctcgtcgcgg tagggaaccg caggcacctc ccggacctcg gcgcccaggg
     5281 tcttcaagag ctgaaccttc tccggggatt gggtgtcggg catgtagatg atcgaccggt
     5341 agcctctggc gttgcagacg tgcaccaggc caatcccggt gttgcccgcg gtgccttcca
     5401 ccaccacgcc gccgggcttg agcgcgccgg acctctcgtg ctcgcggacc atccacagcg
     5461 cggcccggtc cttgaccgag ccgccaggat tcaggaactc tgccttgcct aggatctcgc
     5521 agccggtctc ttcgctcagc cggttcatgc ggatcaacgg ggtgttgccg atggtgccgg
     5581 cgaagccatc tttgatgtcc atggccgatt ttagtcagga tccgattgaa tgcggaagtt
     5641 gccatgggtg atcatcccgg ctcgcgggca agttcaggag atgcgcacag acatgaagaa
     5701 ggccgatctg caggaccgct ggctgggcga tgcgggggaa aacggggtca acccgctgcg
     5761 catcgccgcg ctcatggcgc gggtgcgcaa agaggtggac gaggggctgc tgcccgcggc
     5821 gcaggttgcc ctggccagaa acggcgccct ggtgctgttc gaaacgatgg gcgctgcgga
     5881 caacgactcg ctcttctgcg tcttctcggc aaccaaagcc atcacgtcgg ccgcggcctg
     5941 gatcctcatg caggaggggc gcctgaagga ggatgagctc gtagccgaca tcatccccga
     6001 gttcgccgcg aaccataagc acgatatcac cgtggggcag ctgttcgtgc acaccgccgg
     6061 ctttccgtcc gcgcccttca agcccctgga ctggaacgac cgcgcgcgcc gtttggagcg
     6121 ttttgcccaa tggcagctga actggccgcc gggcagccgc tacgagtacc acccgacctc
     6181 ctcgatgtgg gtgatcgcag agatcatcga gcggcgcgcg aataccacct atcaggagtt
     6241 cgtccgcgaa cgcatcgccc tacccctggg gctgcccgag ctctacgtgg ggctcccggc
     6301 gaccgagaac gagcgggtgg tgccgaacgt gcatgtgggc gatgccctga cgcgagcgga
     6361 ctacgagctt ctcggtctgc cggagccgcc cgtgactgag gtgaccgagg aagcgatcct
     6421 gaagttcaac cggccggaag tccgcgcggt tggggtcccg ggcgggggcg ggatcatgag
     6481 cgcagcggat ctggcgctgt tctaccaggg attgctgcat ggtggcctgg gcgaggaagt
     6541 ctggagcgcc gccacccttg ccgacgcccg tcgcgtgcgc agcggcgaac tccgcgaccc
     6601 ggtgatgggc caccgcgcca atcgagctct gggcctcgtc atcgcgggag atccgcaacg
     6661 gaatctccgc ggtttcggca agaccaactc cccggagacc ttcggccaca acggagccgg
     6721 cgggcagctc gcctgggccg atccggcgac cgggctgtcc ctggcctact gcacggccgg
     6781 tcacgatcgc aaccggatac gccaggggag gcggggcgta gcgatctcga gcctggctgc
     6841 cgtgtgctgc gaagattgac caggacgcaa caccgcgcgc aggaaccgct tgatttgctt
     6901 ctcaatctgc tccctggcgt cggggagcgc ggaacgaact gtgacagacg cctctgtccc
     6961 cgcagggcgg ctgaactaat ctgccgacta cgctgattgc caccggcaca gcgagacgcg
     7021 agaggagtca ccggcccgtg cggcggtgaa cttaacggtt acaccgggat tcgctgtcga
     7081 ttcttcgggg cttccccgac gggtcgagca gtcggcgagt tccggtttta catccggcgt
     7141 cgacccgatg tccgggtcaa ggatctcccg gcatcagccc gaccgccagg acggcggagg
     7201 cgaacagagc aaggactgcg gaaacggtgg ttgccggtag ttcgaaggat ttagccgaaa
     7261 ggatttcggc tccgaaggga cttcggagtg gtgcaaatgg atctcaggag catggagcac
     7321 ccaatctttc gacggcacac tgttgtctcc cccgcgtctg aaagcggcgc atccccgcgc
     7381 ctcatggggt taccaacggg ttgatggtgc ggatctcgca atgttcgcca tcggcggtga
     7441 tggcgtcgaa cccggcgtag atggtgttgt ttgacatggt cacggacacg tttcgcgcga
     7501 atcccagacc gatgcatccc tggcgcaggg tcagcaggta tcgcgtctgc ggactgacct
     7561 ccagcacgac ccgcttgtcg tccactcttg cccagccttc gatccggttg gctgccggga
     7621 tgctcgagac gggggcctgc acagtgccct cgacaatgga ctccgcgcgg gggccaatgg
     7681 gagagtccgg cggcagggcg agtccggacg acgcgtagag cagtactgtc ccggcgattg
     7741 agcaagcaag ttttgcaaat ttcgaggtgc tgggcatgac gttttcctct ggcaagggcg
     7801 acgttcaggc tatgagcgcg acgctgaatg tcggctgaac atgaggcaac ggtacgggag
     7861 cccgtgtgaa aaggtcgtga gaccgcttga gggggctgat gcggttcgag ggttggcaac
     7921 gccggggata ttccgcaaag tgtccacgag gcgaaccagc actgggggta cggtttgatc
     7981 gatcggggag cacatcggcg ccgcgccgca atcctgaatg aggccgcccg gcggatcgtt
     8041 ctgctcaacc tggcgatatt cagcatggcc gcgtcggccg agtccgtcct cgagcgggtc
     8101 gatgcggaag ccgccgccgc cgtatcgatg gccgagcgcc tctgggcgct ggctgagctc
     8161 ggctatcagg aaacccgcag ctccgagctg ctcggggcct atcttgcaga gagcggattc
     8221 tcgctcgaaa gcggcgtggc ggggataccc acggcctttg tcgcgacatt cggttccggc
     8281 cggcccgtca tcggcgtcct cgcggagttc gacgcgctgc cgggcatgtc acaggccgcg
     8341 gaaccctcca gacgacccct ggagcccggc ggggccggcc acgcctgcgg gcatcacctg
     8401 ttcggcgcag gggcggcagc ggcagcggtt tccgtggcgc gctggatgga ggcaaacggg
     8461 gtgcctggct cgattcgcgt gtacggcacg cccgccgagg aaggcggttc cgggaaggtc
     8521 tacctcacca gggcaggcct tttcgacgac gtggacgtcg tgctccactg gcaccccggc
     8581 agccgcaacg acgcctcgcc ggcaaccacc acggcgaaca agtcgggccg cttcactttc
     8641 cggggagtgg ccgcgcatgc cgccgctgcc ccggagcgcg ggcgatccgc tctggacggc
     8701 gtggagtcca tgaactacat ggtgaatctc atgcgcgagc acgtgccgtc tgacagccgc
     8761 atccactacg tgatcaccaa gggtggcgat gcgccgaaca tcgtcccgga aatcgcccag
     8821 gtctactact acgtgcggca tccggaggcg gccaccgtca tcgagctttt tgagcgggtc
     8881 gtcgcggcgg cgcgcggcgc tgcaatgggc accgagaccg acgtggacta cgaggtgatg
     8941 cacggcaact atgcggtgct tcccaacgaa acgctggcca aacgggttca cgcccacctc
     9001 ctgagtctcg ggggcgtgag ctactcgccc gatgagcgcc gatttgccga gcagattcag
     9061 gcgaccctgg gagactcgct gccgccgctc agccgtgccg cgagcgtcga gccgtttcag
     9121 atgcggcaga acatgggctc cacggatgtg ggcgatgtga gctggaacgt gcccaccgtc
     9181 ggcttttacg cggccacctg ggtaccgggc acccccgcgc acagctggca ggcggtggcc
     9241 gcgggcggaa cgagcatcgg ccacaagggc atgttgctgg cgaccaggac cctggcgctc
     9301 accgcggcgg acctgctcag gaccccctcg ctcatcgcgg cggcccgggc ggagctcgaa
     9361 gagcgtcgcg gccaggactt ccgctacgtt gccctgctcg gcgagcgcga tccgcctttg
     9421 gattaccggc ggtgaacctc ggggtcctgc accccgggga gatgggcgtc agcgtgggcg
     9481 ccgttctgac ggccgcaggg caccgggtgt actggcttcc cgagtctcgc agccgcgcaa
     9541 cccgcgagcg cgccgaaggc gccggcttca ccggctgcgc ctcgctccgg gaacttgcgg
     9601 accgcgttgc cggcatcgtc agcgtctgtc ctccggcggc agccgaagag gtggcgagcc
     9661 aggtccgctc ggcgggcttc accggcgtct acgtcgatgc caatgcggtg tctcccgcca
     9721 cggcgaggcg gatggctcga acgatgggtc ccacgtacgt cgacggcggg atcgtcggcc
     9781 ctcccgcggt ccgtcccggc cttgcgcggc tgtacctgtc tggagctgcg gccgaggaag
     9841 tggcgggatg gtttcatggc ggccttctcg aggcccgggt cctggcgggc gaggcgggcg
     9901 cggcgtccgc gctgaagatg tgttacgcgg catataccaa gggcagcagc gctcttgtgc
     9961 tggccatccg ggcgctggcc gaggccgagg gggtggacga gccgctgctg gccgagtggg
    10021 acatctctca gcccggtctt gccgatcgga gccagcgtgc ggccgcgggc acggcggcca
    10081 aagcctggcg gttcgtcggc gagatggagg aaatcgcggc cacctttcgc ggggtgggat
    10141 tgccgggcga gtttcacgag gcggcggcgg ttgtctaccg gcgtctggca ccgttgaaag
    10201 ggaacgcggc ggcagaactc gccgacgtgg ttcgacggct catcgagaat cccgcggatg
    10261 tcggggaaga ttagccgcat cgcccggatg gccgtgtcgt gggccatcgc ggccccggcc
    10321 atcgcggccc cgggctatct gttcgacgag gcggccacgc tcgacctcga gctggaggcc
    10381 cccctcggag cgctcatggg tgaccgctac ccagacagcg agtaccggcc tgcgcagctc
    10441 cgctacgtgg gcaaggacgg tagccgcgtc gcgctgcccg ttgaggtccg gacacgtggg
    10501 aaaacccgcc gggacgagga agtgtgcaag atgccgccgc tgcgtgtgcg cttcgatcaa
    10561 gccgccgcgg acggtacgcc gttcgccggt cagcccgcac taaagctggt cacttactgt
    10621 cgcgacagcg actccttcga tcagctcgtg ctgctcgagt accttgccta cagaacctac
    10681 aacttgctga ccccgcagtc gcatcgggtc cggcttgcgc gcatcagcta cctcgagcgt
    10741 ggggaccggc gaacgacccg ctacggcatc ttcttagagg actggcgggc agtggcagcc
    10801 cgcaacgacc tctccgccga cccggtcgac ggcggtgtga acgtcgacaa gctcaaccac
    10861 gacgccgcca accgcgtggc ggtctttgcg tacctgatcg gcaacgagga ctactcactg
    10921 ctctggccgg aacccgacga gaactgctgc cacaacatca agccgctgct cgaccgggat
    10981 ggccgggtgg tgccgctgcc ctacgatttc gactattccg ggctcgtcaa cgcgccttat
    11041 gccgtggcca agaatccgcg cagaaaagtt cgccagcgag cctaccgggg tctttgcgac
    11101 acccaggaag gtctcgaggc agcgctggag ctcacgcgtg cgcggcgcga ggacatctac
    11161 gcggtcttcc gcgcccagga agggttgtcg ccggcaaagc tgaagtcgtc aatgggctat
    11221 ctcgaccggt tctacgactt gatcgaccag cctgcgcagg tcgaaaaacg cctgcgccgt
    11281 cggtgcagaa aaagctgaac gatcaggcgg ggtccggcgc gatgggtatc tctgcaggga
    11341 catccgcggc ggacggggcg tcaccggagt cgacgtcgat tccggatccc gccggctcgc
    11401 cgcagtccca gcccatggcg ccgagcttgt ctgcgaaagc ggccgcctgg gcctcgcagt
    11461 agccggcttc gttggatgcc cgccacagcg agtggattcc cgcgccttcc tgagtcttgt
    11521 cgtagatcac ttcgcaggga agggtctggt cgggaccgct gtagacgacc tcgacgctgc
    11581 gaaccaggct cccctgagtg cattgcaccg gattggcgaa cgccggagcg gcgatggccg
    11641 agacgatgag gatgacggtc agtttcgttg ctgggtatcc catgagttgc tccttgagat
    11701 cacggccgga accagaaggt cggcggatcg gttctcagtg cctgccgcgc cagcttcatc
    11761 catcggcaca gcgcggggcg ggtttcgcgg gggtctatga tctgctcgat ctcgaagtgc
    11821 tccgcagaac ggaacggcga gcgcagccgg ttgaggcgct ctttgatccg gctgaggtga
    11881 gcatcgtaat cgtcggcctg ggcgaggtcg gccttgtagg ccacctcgat gccgccctcg
    11941 atgggaagcg agccccagtc tcccgacggc cacgccatgc gtacgcaatg gctgccgggt
    12001 ttgtggttgg aagcgccagc gaccccgaac gccttgcgca ccaccacgca gcagaagggg
    12061 acgggtgcct ggcccagagc ggccagcgcc tggcaaccga agcggatggt tccgctctcc
    12121 tcggactgct tgccgatcag aaatccgggg caatcctcca gatgcaccaa cggcaggtgg
    12181 aacgtgctgg ccagatcgat gaaacgcacc agcttgcggc tcgcatccgc cgtccacgcg
    12241 cccccgtaca ccatcgggtt ctctgcaaaa agggccaccg gcatgccgtt cagccgggcc
    12301 agaccggtga tgatggatcg gccccaacgc ctgccgatct cgaagaacga cccctcgtcg
    12361 accaccgagt cgatgatccg gtgcatcttg tagaccttgc gcggatcctt cggaacgatc
    12421 tctagcagcg cctcgtccgt ccgctcggga tcgtccgtgg cgtcggtgac gggcggcagc
    12481 tcgtccgtgc tggtgggcag gtaggagaga aatcgccgtg ccatgtcgaa cgcttcttcc
    12541 tcggaagacg cctcgtcgtc gatggccccg tttcgggtat ggatgcgaga ggcgccgagc
    12601 tcttccttgc tgacatcgcc gaggcttgcc caatccacca gtgcgggtcc cgcgatcatc
    12661 atctgggcgc tgttcttgac gatgacggag tagtggctgg tcgccacccg ggcagcgccg
    12721 ataccggcca cggagcccag cgccagagag accgatgggg caaccgccag gtgctgaacg
    12781 acggtctccc agccgcgcac ctcgggtatg taggttcggc cggccatctc gatggtcttc
    12841 acggatccgc cgccgcccat gccgtccacc agacgcacgt ggggaagccg gagctcggtg
    12901 gccaggccct ctgcctggag ccgtttgccg atgatcgacg cgtccgcgga gccgccgcgc
    12961 accgtgaagt cgtcgcctga gagaatcacc ggccggccgt cgatctccgc ggcgccgaat
    13021 acgaaattgg agggggtgaa cccgatgaga ttgccgtcgt cgtcgtactg cccgacgccg
    13081 gccagggtgc cgatctcctg gaaagagccg gagtcgctga tggcggcgat ccgctcgcgg
    13141 atcgtcagct tgttgaattc gtgctggcgc gccaccttct cggcgccgcc catctgatag
    13201 gcgagctgct cgcgcttgcg cagctcgcga atttcctcct cccagctcat gtggtcggcg
    13261 tccggctgcg gggggcgaag cgccacgcag ccggttcgct cgggctcgga tccgtcaggc
    13321 gcgggttccg gtaaacgtgg cgtcgccgaa agcgccgagc ttgacgtttc cggtcatctc
    13381 gtcaccgttt acggtcgcgc tgaactccag cgtcatcgcc atgggcgtcg tgatgtcagc
    13441 cttccaggcc agcgagtcgc cgtctgccgt gccgtccttg agctcgatct cgccctgggg
    13501 tcctttcagc gtgcccgtca gggtgccgcc gtcggtggcc agggtcatgg tgccgttctg
    13561 ggcgcccatg ggcgtgttca tcacggtgtt ccaggtgccg tctgcgctca tgtgtccgtc
    13621 cctccagtcg ttgcagttga gggctcggag tatggcatag cgggcctggt agacaaacat
    13681 tccggagcct gagttaatgg ccgcccgtga gcatcggcac cctcaacgaa gggcctctgc
    13741 accaggcaat caagtcgctc tacaacggcg ccggcgcccg tgaggaggtg ccggtcggcg
    13801 acttcgtagc cgacgtcttc ggccccgacg aggtcatcta cgagattcag accggcggtt
    13861 tcggcagcct caagaagaaa ctcgctcgcc tggtggagga tcatcgcgtc gtcctggtcc
    13921 atcccatcgc agaggtgcgc tacatcgtca agctgcccca gggggaagac cagcaggcca
    13981 gccgtcggcg ctcgcccaaa cgcggcagcg ttgcccacgt gctcgaccgg ctggtgagca
    14041 ttcctgcgct tctgaatcat cccaacttcg agctcgaggt tctgctgatc gaggaagagg
    14101 agttgcggac cttcgatccg cgcagggtcc gccgacggga tggatggcgt gtggtgcagc
    14161 gccgtctggg cagcgtgctc gcctccgagc gtttcacctg tgctggcgat cttctgcgca
    14221 tggtgcccgg gccgcttcct gcggagttca ccaccgagga tctcgccggc gcgatgggtc
    14281 agccgcgctg gcttgcccag aagctggctt actgcctgcg cgaggccgga gagatcagca
    14341 tctccggcaa gcggggcaac gccctgtgct accggctagc gcgttcgggc gccgtggctt
    14401 gacatgcggc ccgggggcct cgaatactcc ttcgcccgcc catcagcgag catacccttg
    14461 atcgagcttt acaccgcgtc tacgcccaac ggctggaaag cgtccgtcac tttggaagaa
    14521 ctcggccttc cctacgaggt acggcccgtc gacctgatgg ccggcgagca gaaggccccg
    14581 gagtttctga agatcaaccc gaacggcaga gttcccgcca tcgtggacga cggttttccg
    14641 atattcgagt ccggcgccat catgattcac ctggctgaga aaaccgggcg cctcatgccg
    14701 gctgacgctc atggacgttc tctggtcatc cagtggctga tgttccagat gggcggcgtc
    14761 ggccccatga tgggtcaggc gaacgtcttc tatcgggcaa tggaggaaaa gatccagccc
    14821 gccatcgacc gctaccagaa cgagtgtcgg cgcttgttcg aagttctcga tggccgtctt
    14881 cggaacagcg agtggctggc cgaccacttc tcgattgccg atatcgccaa ctggtgctgg
    14941 gcgcgccttt actactggtc gggtgtttcc ctcgacgggc tcgacgccat gcagcggtgg
    15001 atggccgcca tggaggcgcg tcccgcctgt cagagaggcg ttgaggttcc caaccggctc
    15061 gagctgcctc gagacgatga tccggacggg atcgaacgcc aggcactcca gtcacgccgc
    15121 gtgctggtgc gctgatggcc ctcgaatcgg taatcgaagg gccggcctgg gatctgaccg
    15181 gcgagtactc gtcgcccgac tctcagggct tgcaggccga cctcgaccga ctcagcgagc
    15241 tgctcgatcg gatcgaggcg ctcaacgggc agcttaatgg ctctggcgct gttgcggccg
    15301 cccagcagat tcaccgtctg gccgatcagg cccgggagct gctggcggac ccacgcgctt
    15361 acgcctcctg cctgctctcg gtgaacggcc gggacgaagc cgccctcatg ctgcaggggc
    15421 gtttgcaggg ttaccagacg cgcttcgggg atctgctcaa gccgctggag ctgttcatcg
    15481 atctggcgga cgacgagggc atcgaagcgt ttctggccga cccggaggtg gcgccggccg
    15541 agttcgtggt gcgccatgcg cgcaagcggc gccatgagcg tctggggctg gccgaggaac
    15601 agctcatcaa cggcctctcc caggagggca ttcacgcctg gggcaatctc tacgatcagc
    15661 tctccggcac gctcacctgc gaagtgctgg tgggcaacga gacccgggag atgggcatcg
    15721 ccgaagcggc cggcctgctg cagaagcccg acgtggcgac ccggcggaac gcctggcgcg
    15781 cgatcaacga ctcatggtcg gcccatgccg aaacctgtgc tgccagtctc aacgccatcg
    15841 ccggttggcg gctggagctc gggcgccggc gcggccgcga agctcccgta cacttcctgg
    15901 atgcgccggt gcacatgaac cggatcagcc gcgaaaccct ggataccctg atggacgtgg
    15961 ctgccggctc gcggcccctc gcccggcggg cggcgcggct gatggcccgt gcttacggcc
    16021 tggaaaccat cggtccctgg gaccagcgcg cgccggcacc cgaccttggt acggcccccg
    16081 ccgcggggat cccgtatgac caggcgctgc acaccatcgc cgatgcctat gcggacgtgc
    16141 atccctccat gggcgagttc gtccgcatga tgcatgccaa ccgatggatc gaggggaccc
    16201 ggggccccaa caagcgtccg ggcgcctact gcacgggctt cgccaagtcc cgcacgccgc
    16261 gtatctacat gacctactcc ggtggctcca gcgacgtcat caccctggct cacgagcttg
    16321 gccacgccct gcacagctgg gtcatgcggg acctgccgaa cagccagcgc ggctacggga
    16381 tggcgctggc cgagacggca agcacttttg gtgagactct ggttcgggac gcgctcctcg
    16441 gcagtgccgg ctctccccag gcaaagctcg acatggcctg ggaagagatg gcggcgctgg
    16501 tgagcttcgt cctcaacatc cccacccgtt tcgagttcga acggcgtttc tatgaagccc
    16561 gccgggagcg gcccgtgctg ccccgggagc tcaacgccat gatggcggat gcgtggacag
    16621 agtgtttcgg cgacgtgctc gaggagcccg acccgatgtt ctgggccagc aagctgcact
    16681 tctacatatc cggcatcagc ttctacaact ttccctacct gttcggatac ctgttcagcc
    16741 tgggcgtcta cgcgcggcgc ggcgacggtg ccgacttcta cccccgttac gaagggctgc
    16801 ttcgggacac cgggcggatg acggccgagg cgctcgcggc caagcatctt tccgaggacc
    16861 tgtcgcggcc cggtttctgg gaggccacgg tcgcatctct cgagacccgg gtcgatgctt
    16921 tcgaggatct gctcgacgag ctggcgctct agctttcctc ggcgaaggcc actgccagga
    16981 cgtcgtcgag gtgggcggcc agcgagatct gcatctcgtc cagaaccgtc tgcgggagct
    17041 tggcaaggtc ggcttcgttg tctttgggca ggatgacccg acgcagcccg gcgcggtgcg
    17101 cggcgaggat cttctccttc accccgccga cgggcagcac caggccggac agcgtcagct
    17161 ctccggtcat ggcgacgtca tcgcgaaggg ccctgccttt gtatgccgag tagagcgcgc
    17221 acgccatggt gacaccggcg gaaggtccgt ctttcggcac cgcaccggct ggaacgtgta
    17281 tgtgcacacc gctttcctcg atggcgggcc tgtccaggcc gagcctgtcc gccgctgacc
    17341 acaggtggct gcgggccgcc ctggcagatt cctgcatcac ctgcccgaga tgcccggtga
    17401 gcgtgacctt ctcgtccttc tgggtgagcg tcgcttcgac gtagagcacg tccccgcccg
    17461 cctcggtcca ggccagcccc gccgcgacgc cgggctccag cccggtgcgt ccgcggtcgg
    17521 atttgaatcg ctccggtccc agcagttcgg ccagatcgtc ggctgcgacc ggtgtgtcca
    17581 ggctctggcc gtccaccacc tgacgcgcgc gctttctcgc cagacggccg atgacgcgct
    17641 ccagcccccg cacgcccgct tcacgggtgt agcggcggat catggtggtg agcgcgtcgt
    17701 ctgagatatc cagctgagcc tcggaaaggc ccgcctcgct gcgctgtcgg gggatcaggt
    17761 actggcgcgc aatggcctgc ttctcgaggt cgctgtagcc ggtgagatcc agcacctcca
    17821 tccggtccag cagcggccgc gggatcccct ccagggtgtt tgccgtggtg atgaagagga
    17881 cgcgagacag gtcgtaaggc agattcaggt agttgtccct gaactcgcgg ttctgggcgg
    17941 gatcgagtat ctccatcagg gccgcggcgg ggtcgccgcg aaagtcgcga ccgagcttgt
    18001 ccacctcgtc gagcatcagg atcgggttgg tgacgccggc gcgccggacg ccctggagga
    18061 tgcggccggg catggcgccg atgtaggtgc gccggtgtcc tctgagctcc gcctcgtcgt
    18121 gcagaccacc gaggctcaac cgctcgaact tgcgccccat ggcccgggca atggactggc
    18181 ccagggacgt cttgcccacc ccgggcgggc ctacgaagca gagaatgggc gctttcgcgc
    18241 cagggttcag ctgcatgacg gccagagatt cgaggatgcg ctccttgacg tcctccagac
    18301 cgtagtgatc ttcgtccaga atttccctgg cgtgcttcag atcgagctga tcctcggtct
    18361 cctgatgcca gggcagctcc ccgattagct ccagataggc ccgggcgacc tggtagtcgg
    18421 cggcgttcgc cgacatgcgg gcgagccgct tcagctctct gtccacttcc gcctgaacgg
    18481 gctccggcag gcgggcctcg tcgagctggc ccttcagctc ggcaatgtca tcgtcctccc
    18541 ccgactcccc cagggcctgc tcgatggcgc gtttctgctg gcgcaggacg tgttcacgct
    18601 gctgctgatc catgtcgtcc cgggcccgtt ccgcgatctg cctgcgcagc tcggtgacct
    18661 ggagctcgtg tcccagcagc tcgtggatgg cttccatcag ggcgagcgtg gtgtcggcct
    18721 cgagaattcg ctgctcctgg atcagctcga tccccggggc gagggacgcg atgcggtaca
    18781 tctgagtgat gggatcggag atcgagccca ccagctgctg atagatctgc gcgccgttct
    18841 cgctgtccat gatctctgcg atgcgctggg cgagctggag gttctcccgc agggtagcgt
    18901 ccacggccgg gccgtcggtc ccgaggtcgt tcatgccgag acccggcagc tcctcgtact
    18961 gcgcggcgag gaacgcgccc tcgtcctccg cgattccggt caaccgtacc cggcgcgtcc
    19021 cctgaacgat cacctgggcg ccgccgtcgc gattctccac gcgactgatg ctggcgatgc
    19081 agccgacggc gtagagatcc gtcaggcccg gctcctcgtt ggcgccatcg tgctgggcaa
    19141 cggtgatgat ctccctgggc ccctccaccg cgtggttcag ggccgccaga gagcgacgcc
    19201 gacccacgcc gagggggcgg acgatctgcg ggtagacgac cgtgtcttta aggggcagga
    19261 cggggaggac tggcatggcg ttctcgtcag gcgcacaggc cctgaaggcg ggctgcctgc
    19321 tcgcgcaaca actccggagc tccgagcatc tggcgattgt agccgatccg cttgaaccag
    19381 tagtggagtc cgagaaggtc cgtgaagccc atgccgccgt gtacctcggt cgccgtcttg
    19441 gcgacgaact tgccgacctc cgaaaggtgg gccttggcgt ggcaggcgca cagggaggct
    19501 tcggcgggaa ggtcgtcgag cgcgtgggat gcgtaccaga ccatggctcg gcacggttcg
    19561 aggctcgcgg ccatctctgc gcacatgtgc ttgacggcct gaaacgagcc gatgggccgg
    19621 ttgaactgct ctcgctgcat cgcgtattcc accgcttgat cgagcatgtt ctgggcagcg
    19681 ccgagggtgt ccgctgcgag catgacccgt cccgcatcga tcacccgggc gagcacgtcc
    19741 gggtcgtcgc agatcagctg tgccggcgcc ccgttcagcg tgagcgcgcc ggtgcggcgg
    19801 gtcttgtcga tggttggaag cgcttcggcg aacaccccgg gcgctccggc gtcgacgagg
    19861 taggtgctcc ctgccgcgtc cgcgaccagg taagcctggg catcgaaatc cagaacggag
    19921 agcgctttgc cggagagcgt ctccccgtcg gcggtgatgc cgccatccgc ccgcgcgccg
    19981 acgaactcgg ccacggcgac cccgaccgtg agttctcctg tcgccagtcg ggacagggta
    20041 tccgtcggct cgttgcccgc cagcgtgagg gccagcggcg ccatgacggc ggaacctaga
    20101 aaagcgcaag gggcggtgtg gctgccaagg gcctcggcga ccagcgccgc ctcgaagcag
    20161 cccaggccca ccccgcccgc ttcctccggg acgagcagcc cgaagacgcc gagctccgcc
    20221 aggccggaat gaaggtcggc cgccacaggc tcctcggcaa agcggcgtac cctatccaga
    20281 gggcagttgg cgtccaggaa gccgttcaag ctgtcctgca acagtgtctg ctcttcggaa
    20341 agcccgaact ccatggcggc ctcctaagcc tgcttttcgt atttgggttc tttcggcatg
    20401 ccgagcccgc gttcgctgat gatgttcttc tggatctgcg aggtgccccc gccgatgatc
    20461 agccccaggt agaacatgta ctggctctgc cagctgccgt gatcgcgcag gtaggggttg
    20521 tcgccgtagg ccagccccag ctctcccatg gcgtccacgg ccagaccctc gaggtcgtgg
    20581 cgcagctcgg tgccctgcag cttgacgatc attcgtgcga ggcgggcatc cgctgcgttg
    20641 accttggcgg acagcagccg aaggtcgttg tagctcagcg ccatgacccg accctggatc
    20701 tgcatgaggc ggtcccggaa caccggatgg tcgatcagcc gctccccgcc gagggtctcc
    20761 gtcttcatga gctctgtaag ggcgttaaat cgggagagca tgacgttcgg gtcgcccaga
    20821 gagtcccgct cgtggccgag gatggcgttg gcgaccagcc agccctcacc gcggcggccg
    20881 acgatctgat cttttgggac ccggacgtcg gtgaagaacg tctcgttgaa gttcgccgcg
    20941 ccggtcatgt ccaccagcgg gcggacctct atgccggggg tgtccatggg cagcaggaga
    21001 aagctgatcc cctggtgctt gggcgcatcg ggctcggtcc ggaccaggca gaacatccag
    21061 tccgcgatgt gcgccgtgct ggtccagatc ttctgcccgt tgatcaccca ctcgtcaccg
    21121 tcgagctcgg cgcgcgtgga caggctggcg aggtcactgc cggccccggg ttccgaatat
    21181 ccctgacacc acagcatctc gccgcgcagc gtgggcggga tgaaggtggt tttctgctcc
    21241 tgcgtgccca tctccagcag ggtcggcacc agcatggata tgccctgacc gccaagcccg
    21301 gagctcacgc gcgcggcggc gaactcctcg gcgatgatcc gtgattcgag aatgtcgggc
    21361 gcggcgccgt agcccccgta ctcgcgtgga atcgttcttg ccgcgtagcc gttttcgatg
    21421 agcaggtgct gccaggcgag tgttttctcc gaccggatgt gctcgccgcg cggggcgctc
    21481 ccggcgtggt cggcgagaaa cgatctgacc tcctcgcgga actcttcgta atgtggaccg
    21541 taggaaagat ccataccaat ctcccgggcg gaatcagggg cgcaaaacag ggtgcggtcg
    21601 acaacataac cgctaccgga aggggcggca acttggccgg tcgggcaggg ggttcaggcc
    21661 aggcggtcga gctgttcccg ccttacccgg tacttgagcg gcacgccgcg accgtagcgt
    21721 tcgagctcct cgatgatgca atcggtgagc cgctgggtct ccgtccccag cgagccggcg
    21781 atgtgtggcg tcaggaagac gttcggcaac ccgtacaggg gtgattccgg cggcagctcc
    21841 tcgggatcgg tggtgtccag cacggcgtag aggcggccgc gcgtgagctc tgcctccagc
    21901 gccgcctggt cgatgagagc gccgcgcgcg gtgttcacga tggtcgcgcc gtcgtgcatc
    21961 agcgcaagct cgcgtgcccc gatcatgtgg cgcgtctctt tcaacagcgg cgcgtgcagg
    22021 ctgaccgtgt ccgattgcga cagcagctcg gagagcccca ccttggtcgc gccgaggtct
    22081 cgcgcggcaa ggggcgtcac gaacggatcg tagagcagca gacggatgtc gaagcgccgc
    22141 aggtgctcca tcacaagcct accaacgtga gaagcgccca cgatgccgac gctcttgtgg
    22201 tagttgccta cgtccggcgc ctccttcgtc caaggtgggc ggtgctcccg atgctcgaga
    22261 tagacgcgat tgagctgaaa cacccgcttg ttcgcga
//
LOCUS       DBNHLM010000292         5398 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1479390, whole genome shotgun sequence.
ACCESSION   DBNHLM010000292 DBNHLM010000000
VERSION     DBNHLM010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1479390"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="AC16QF_13580"
     CDS             complement(<1..697)
                     /locus_tag="AC16QF_13580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017736997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6449639.1"
                     /translation="MREFEDFRDKVAAITGAASGMGRALAVALAERGCHVAIADVHAD
                     GLDETCRRVQAVAGVRCSTHLVDVADRAAVEAFAADTVREHGGVDLIFNNAGVDVTAT
                     VEEMSYADFEWLVNINFWGVVHGTKAFLPYLRQAGQGHVVNTSSLFGLMSVPTQSAYH
                     AAKFAVKGFTDSLRLELADTDIGVSCVMPGGVRTNIVRNARFRISDNQAPTQDEVVER
                     FESRAGLSSEQAAE"
     gene            794..1282
                     /locus_tag="AC16QF_13585"
     CDS             794..1282
                     /locus_tag="AC16QF_13585"
                     /inference="COORDINATES: protein motif:HMM:NF019003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HIR6449640.1"
                     /translation="MSRPYVDYIQRTREQYDGLGYPPYQWVSNDDEPPFQPLAKPLAD
                     CRVGLIASGGIYRHGQVAFHFRDDLSFREIPRDTEVSELRATHFAYDLTDARKDPNVV
                     FPMETLRDLARTGRIRELSANAYTFMGGIYSARKVRDILAPAIASRLQKDEVDVAILV
                     PV"
     gene            1322..1723
                     /locus_tag="AC16QF_13590"
     CDS             1322..1723
                     /locus_tag="AC16QF_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449641.1"
                     /translation="MESRGIPTICMSSAYSITAAVNPPRAVYLDFPLGHTAGKAGDKA
                     LQRRVMIDTLSALDGILAPGTIRELPYEWSSDDAWKDQAMRPKEGSRPSDDRVERHDT
                     PQYQTAEDRDAAEAQLATGQCPGCVWLSETT"
     gene            1862..2488
                     /locus_tag="AC16QF_13595"
     CDS             1862..2488
                     /locus_tag="AC16QF_13595"
                     /inference="COORDINATES: protein motif:HMM:NF022426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HIR6449642.1"
                     /translation="MPRTLATILLAGTAALGGCASLSESQCLAGDWQTVGYRDGSSGQ
                     NRAALLEHQNACVKHGVTPDREAYLAGWDQGILQYCQPRNGFALGERGAAFPTLCPGA
                     VVDPFEAAYHDGRRLYLARAEIRRLEREINAREHRLEHLDEAIASAESRLVEDGLSSA
                     QRRELLEETKHMSREQGEIEVELKELRIDVALEHQRLAQLRHALASAS"
     gene            complement(2538..3767)
                     /gene="nqrF"
                     /locus_tag="AC16QF_13600"
     CDS             complement(2538..3767)
                     /gene="nqrF"
                     /locus_tag="AC16QF_13600"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HIR6449643.1"
                     /translation="MLDTTIILGVVMFTVVVLFLVMVILFARSRLVSTGEITIEINED
                     PENTIVVPAGGKLLSTLASKGIFLASACGGGGTCAQCRCQVLDGGGSILSTEEGHFTR
                     GEIRDHFRLSCQVAVKQDMKIHIPEDAIGVQRWECEVTSNPNVATFIKELNLKLPEGA
                     NVDFRAGGYVQLEIPPYEMDYKDIDVEDEYQEDWDRFDVWKYHSKVTEPTIRAYSMAN
                     YPEEKGILKFNIRIASPPPGMDVPPGNMSSYVFGLKPGDKLTVFGPYGDFFVKDTPAE
                     KIWIGGGAGMAPLRSQIFDELKRRKQSYTMSYWYGARSLREMFYTEEFDDLASEYDNF
                     EWHVALSDPLPEDEWTGHTGFIHQVVYDNYLKDHPNPEDCEYYLCGPPPMVAAVLQML
                     EDLGVEPENILLDDFGG"
     gene            complement(3778..4386)
                     /gene="nqrE"
                     /locus_tag="AC16QF_13605"
     CDS             complement(3778..4386)
                     /gene="nqrE"
                     /locus_tag="AC16QF_13605"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HIR6449644.1"
                     /translation="MEQYLGLFVRAVFVENMALTFFLGMCTFIAISKKIATAIGLGVA
                     VIVVLAITTPVNNLIFNYLLRDGALAWAGFPDVDLSFLGFLSYIGVIAAMVQILEMFL
                     DRFVPVLYNALGVFLPLITVNCAILGASLFMVERDYTFAESVVFGLGAGVGWALAITA
                     LAGVREKLKYSDVPEGLRGLGITFISVGLISLCFMSFGGIDI"
     gene            complement(4389..5051)
                     /locus_tag="AC16QF_13610"
     CDS             complement(4389..5051)
                     /locus_tag="AC16QF_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HIR6449645.1"
                     /translation="MATTKEVLVDPIFNNNPIALQVLGICSALAVTVSMSTTLVMCAA
                     VVFVTTMSNVAVSLVRNRVPTNIRIIVQMTIIASLVIVVDEVLKAVAYDISKSLSVFV
                     GLIITNCIVMGRAEAFAMQNGPLMSALDGLGNALGYSVILIGVALIREPLGSGTLLGF
                     EILPRVMDGGWYHANGMMLLAPSAFFIIGLFIWAIRSWKKGQVEESEYEIAPNVQYGE
                     AI"
     gene            complement(5069..>5398)
                     /locus_tag="AC16QF_13615"
     CDS             complement(5069..>5398)
                     /locus_tag="AC16QF_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR6449646.1"
                     /translation="YGYLALEGDLNTVAGLGFYSQKETPGLGGEVDNPSWKGQWPGVE
                     LFNAAGQPAVRLVKSRSPSASAAAVHEVDALAGATLTTRGVENLVRFWTGELGFGPFL
                     ASLKANS"
BASE COUNT         1066 a         1756 c         1622 g          954 t
ORIGIN      
        1 actctgcggc ctgctccgag ctcagccccg cccggctctc gaaccgctcg accacttcgt
       61 cctgggtggg cgcctggttg tcagatatcc ggaacctcgc attgcgcacg atattcgtcc
      121 ggacccctcc gggcatcacg cagctcacgc cgatgtccgt gtcagccagc tcgagcctca
      181 gagagtcggt aaagcccttc acggcgaact tggcggcatg gtaggccgac tgcgtcggca
      241 cggacatgag cccgaacagg ctcgacgtgt tgaccacgtg cccttgccca gcctgccgga
      301 ggtagggaag gaacgccttg gtgccgtgca cgacccccca gaaattgatg ttcacgagcc
      361 actcgaagtc tgcgtagctc atttcctcca cggtggcggt cacgtctacc cccgcgttgt
      421 tgaatatcaa gtcaacgccg ccgtgctcgc gcaccgtatc cgctgcgaac gcctccaccg
      481 cagcacgatc ggcaacatcg acgaggtggg tggagcatcg cacgccggca acggcctgca
      541 cccgcctgca cgtctcgtca agcccgtcgg cgtgcacgtc ggcgatggcg acgtggcagc
      601 cgcgctcggc aagcgccacc gcaagagcgc gtcccatgcc ggatgccgcc ccggtgattg
      661 ccgccacctt atccctgaag tcctcaaact cgcgcattga cctcgacgca gtgtggttcg
      721 cgttcacctt tgcatttaat gatcgcggca aaagggatgc aatggcgggc aaaggccgta
      781 tgatgagaaa acgatgtcga gaccatacgt cgattacatc cagcgcaccc gggagcagta
      841 cgacggcctg ggctacccgc cgtatcagtg ggtaagcaac gacgacgagc cccccttcca
      901 gccgctggca aagcccctgg cagattgccg ggtcggcctg atcgcctcag gcggcatcta
      961 ccggcatggt caggtggcct ttcacttccg ggacgacttg agcttcaggg aaatccctcg
     1021 tgataccgag gtatccgagc tgcgagccac ccacttcgcc tacgatctca ccgacgcgcg
     1081 caaagacccc aacgtcgtgt ttccgatgga gacgcttcgc gatctcgccc gaaccggacg
     1141 cataagggag ctctccgcca atgcctacac gttcatgggc gggatttact cggcccgtaa
     1201 ggtccgcgat attctggcgc cggccatagc ctccagactg cagaaggatg aggtggacgt
     1261 ggccattctg gtgccggtct gacccgtctg ccatcagtcc gtcgggctga tagcaagaga
     1321 ggtggaaagc cggggcattc ccaccatctg catgtccagc gcctactcca tcaccgcagc
     1381 cgtcaatccg ccgagagccg tgtacctgga ctttccgctg ggtcacacgg caggaaaggc
     1441 cggcgacaag gccctgcagc gccgcgtgat gatcgacacc ctgtcggccc tggacggcat
     1501 tctcgccccc ggcaccattc gcgagctgcc gtacgagtgg tcgtccgacg acgcctggaa
     1561 agaccaggca atgcgcccga aggaggggtc gcggccttcg gacgaccgcg tcgagcggca
     1621 cgatacgcct caatatcaga ccgccgagga ccgggacgcc gccgaggcgc agctcgccac
     1681 cggccaatgc cccggctgcg tctggttgtc cgaaacgacc tgattccgac cgcggcccgg
     1741 ttccggccgc cctgcacctc cgggcgggct gccaaagtgt gaactgattg gcggtccggc
     1801 tcgcgcgttt gggccatctt tccccgctga ccctcaggaa gcatgtgaca acggaggtcc
     1861 tatgccccgg accctagcaa ccattctcct cgccgggacg gccgccctgg gtgggtgcgc
     1921 cagcctgagc gaaagccagt gcctcgccgg cgactggcag accgtcggct accgggacgg
     1981 gtcaagcggc cagaaccgcg cggcgcttct cgagcaccag aacgcatgcg tcaagcatgg
     2041 ggtgaccccc gatcgcgaag cctacctggc cggttgggac cagggaattc ttcagtactg
     2101 ccagccgcgc aacgggtttg ctttaggtga gcgcggcgcc gcttttccga ccctttgtcc
     2161 gggcgccgtg gtagacccgt ttgaagccgc ctaccacgac ggtcgccggc tttatctggc
     2221 gcgggcggaa atccggcggc ttgagcgcga gatcaatgcc cgcgagcatc gcctcgagca
     2281 cctcgatgag gccatcgcgt cagctgagag ccgtctggtg gaagatgggt tgagcagcgc
     2341 gcagcggcgg gaactgctgg aggaaaccaa gcacatgtcc cgcgagcagg gcgagatcga
     2401 agtcgagctc aaggaactgc gcatcgacgt cgctctcgag caccagcgcc tcgcgcagct
     2461 tcgtcacgcc ctcgcatcag cgagctgatc cagcacgtcg gcgcccgggt gctttcgcgt
     2521 cgcgctacaa aacccgacta acccccgaaa tcgtcgagca gaatattctc cggctcgacg
     2581 ccgaggtcct cgagcatctg caggacggcc gccaccatgg gcggaggccc gcagaggtag
     2641 tactcgcaat cttccggatt gggatggtcc ttgaggtaat tgtcgtacac cacctgatgg
     2701 atgaatcccg tatgacccgt ccactcgtcc tcgggcaacg gatccgacag ggcgacgtgc
     2761 cactcgaagt tgtcgtactc gctggcaaga tcgtcgaact cctcggtgta gaacatctcc
     2821 cgcagtgatc gggctccgta ccagtagctc atcgtgtagc tctgcttgcg ccgcttgagc
     2881 tcgtcgaaaa tctgcgagcg cagcggcgcc atgccggcgc ccccgccgat ccagatcttc
     2941 tcggcaggcg tgtctttgac gaagaagtca ccgtagggcc cgaataccgt gagcttgtcc
     3001 ccgggcttga gcccgaagac gtaggaagac atattgccgg gcgggacgtc catcccgggc
     3061 ggcggcgagg caatacgaat gttgaacttt aggatcccct tctcttccgg gtaattggcc
     3121 atggagtatg cccggatggt gggttcggtc accttgctgt gatacttcca gacgtcgaac
     3181 cgatcccagt cctcctggta ctcgtcctcc acgtcgatgt ccttgtagtc catctcgtaa
     3241 ggcggaattt ccagctggac gtaaccgccg gctcggaaat caacgttggc gccctccggg
     3301 agcttcaggt tgagctcttt gatgaaggtc gccacgttgg ggttcgaggt cacttcacac
     3361 tcccatcgct ggacgccgat ggcgtcctcc ggaatgtgaa ttttcatgtc ctgcttcacc
     3421 gccacctgac acgaaagccg gaagtgatca cgaatctcgc cgcgggtgaa gtgaccctcc
     3481 tcggtggaga ggatgctccc gccaccatca agaacctggc aacgacactg ggcgcaggtc
     3541 ccgccgccac cgcaggcgct ggcgaggaaa atccccttgg aggccagggt gctgagcagc
     3601 ttgccgcccg ccggcaccac gatggtgttc tcaggatcct cgttgatctc tatggtgatc
     3661 tcgccggtgc tgaccaggcg cgagcgggcg aacaggatca ccatgaccag gaaaaggaca
     3721 accaccgtga acatgaccac accaaggatg atcgtcgtgt ctaacatcta accgtcctta
     3781 aatgtcgatg ccaccgaagg acatgaagca cagactgatc agccccacgg agatgaatgt
     3841 gatacccaga ccacgcagcc cctccggcac gtcgctgtac ttgagcttct cccgaacgcc
     3901 ggcgagcgcc gtgatggcaa gcgcccaccc gacgccggcc ccaaggccga aaacgacgct
     3961 ttcggcaaag gtgtagtcgc gctccaccat gaacagggac gctccgagaa tggcacagtt
     4021 caccgtgatc agcggcagaa acacgccgag cgcgttgtag agcacgggca cgaaacggtc
     4081 caggaacatt tccagtatct gcaccatggc cgcgatcacg ccgatgtacg agagaaagcc
     4141 gagaaagctc aagtccacgt cggggaaacc ggcccaggcc agcgctccgt cacgcagaag
     4201 atagttgaag atcaggttgt tgaccggcgt cgtgatggca agcaccacga tgacggcaac
     4261 gccgaggccg attgccgtcg cgatcttttt ggagatggcg atgaacgtgc acatgcctag
     4321 aaaaaacgtc agggccatgt tctcgacgaa gaccgcgcga acgaaaagcc ccagatactg
     4381 ttccagcatc agatggcctc cccatactga acgttcggcg cgatttcgta ctcgctttcc
     4441 tccacctgcc cctttttcca gctgcgaatg gcccagatga aaaggccgat gatgaaaaat
     4501 gcgctggggg ccagcagcat catgccgttg gcgtgatacc acccgccgtc catcaccctc
     4561 ggcagtatct cgaacccgag cagagttccc gagcccagag gctcacgaat gagcgcgacg
     4621 ccgatgagga tgacgctgta acccagcgcg ttgcccagcc cgtcgagggc ggacatgagc
     4681 ggcccgttct gcatcgcgaa tgcctccgct cggcccatga cgatgcagtt ggtgatgatc
     4741 aggcccacga agacggacaa actcttggag atgtcgtaag ccaccgcctt gagcacctcg
     4801 tcgaccacga tcaccagaga ggcgatgatg gtcatctgca cgatgatccg gatgttggtc
     4861 ggaacccggt ttcgcaccaa cgacacggcc acgttggaca tggtcgtcac gaacacgacg
     4921 gcggcgcaca tcaccagcgt ggtggacatg ctgaccgtga ccgcaagcgc ggagcagatg
     4981 ccgagcacct gcagggcaat gggattgttg ttgaagatcg gatctacgag gacttctttc
     5041 gttgtcgcca tctttcctta ccctcgcctc atgagttcgc tttcagactt gccagaaatg
     5101 ggccgaagcc cagctcgccg gtccagaacc ggacgaggtt ctcgacgccg cgggtagtca
     5161 gggtcgcacc ggccagagcg tcgacttcgt ggacggcggc ggcgctggcc gacggcgagc
     5221 gcgacttgac caggcgcacg gccggttgac cggcggcatt gaatagctcc acgcccggcc
     5281 attgcccctt ccagctcggg ttgtccactt cgccgccgag ccccggcgtt tccttctggg
     5341 agtagaagcc aaggccggca acggtgttga ggtcgccctc cagggcgaga tagccgta
//
LOCUS       DBNHLM010000293         5882 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1240653, whole genome shotgun sequence.
ACCESSION   DBNHLM010000293 DBNHLM010000000
VERSION     DBNHLM010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1240653"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="AC16QF_13620"
     CDS             <1..527
                     /locus_tag="AC16QF_13620"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR6449647.1"
                     /translation="TPSIAEPAQGWFDVAIYEMKETIGPVVEQYGSETLKQIFHWYYD
                     FGDLVDADGYREAIAARTTLTRRWNLFLDEYPLVLSPFLMRPTYPWNYDAVSYEQAHD
                     LFNSGIYSFGINYLGLPAGVVPVGLVEGLPAGVQIVGRRFREDLILDAMEAVEARVGV
                     LAHELWRSEAPAAS"
     gene            535..1215
                     /locus_tag="AC16QF_13625"
     CDS             535..1215
                     /locus_tag="AC16QF_13625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR6449648.1"
                     /translation="MRSISVLIPVLNDAAALRRLLDRLEALVSESIIGCEIVVIDGGS
                     TDGSQNVCAERDCLVIQSAGGRGRQLQAGFEASSGDLIWMLHADTDPDSEALKYLVTL
                     DAPAWGRFDVRFADGPPFLSVVATLMNWRSRMTGIATGDQAIFAHRSLLEAIGGVPRQ
                     ALMEDIELSKRLKTRMRPLCSRHIVETSGRRWEARGVIRTILSMWRFRLRYWIGADPE
                     RLAAEYYS"
     gene            1212..1826
                     /locus_tag="AC16QF_13630"
     CDS             1212..1826
                     /locus_tag="AC16QF_13630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR6449649.1"
                     /translation="MSHLGTLCIFARAPERGAVKTRLAARIGADAALAAYQRLVDLAL
                     SRYAALPDLRSELWVSGDRCLPEVRGWSADWGLPLFEQRGGDLGERMAAAMAHGCARD
                     VATLIVGIDCPCIDGAYVRAAAQTLLTTDLVLAPAEDGGYGLIGLRRPMPELFRGVAW
                     GSSSVLAETLVKAERLRLRYELLETVWDVDDAADWERFLESGYG"
     gene            complement(1858..2274)
                     /locus_tag="AC16QF_13635"
     CDS             complement(1858..2274)
                     /locus_tag="AC16QF_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HIR6449650.1"
                     /translation="MDIGDIRDSFAFFDSWEDKYRFVIEIGRDLPPLDAALKTEDNLV
                     RGCQSQVWLVAAYDPVADRLTFAIDSDAHIVRGLIAIILAAFDKRSPAEILAFDVEAL
                     FEELELLQHLSPSRGNGLRAMVGRIRGIAAENAAGA"
     gene            complement(2278..2802)
                     /gene="sufT"
                     /locus_tag="AC16QF_13640"
     CDS             complement(2278..2802)
                     /gene="sufT"
                     /locus_tag="AC16QF_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HIR6449651.1"
                     /translation="MEKRIIATTRECPARLVPTGDPITIPSNTFVTLTQNLGGTYTVI
                     MNGNMARVDSADADALGFEPQTLEFEPPADGKVDMEQVKAALATVYDPEIPVNVVDLG
                     LIYDCRLTDRHVHIAMTLTAPGCGMGPVLVEDIKSRVGRVPYVESVDVELVFDPPWSR
                     DMMTAEAQLELGLF"
     gene            complement(2817..3173)
                     /locus_tag="AC16QF_13645"
     CDS             complement(2817..3173)
                     /locus_tag="AC16QF_13645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIR6449652.1"
                     /translation="MSVRTFEPTQIAVTPAAAAHIRSQIATSGHKYLRLGVKESGCNG
                     YMYTLDYLDEPAENDRAFEVADSLSLYVSTDDLPMVSGTEVDYVTEGLNSSLKFKNPN
                     AESHCGCGESFSIAGS"
     gene            complement(3205..4419)
                     /locus_tag="AC16QF_13650"
     CDS             complement(3205..4419)
                     /locus_tag="AC16QF_13650"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR6449653.1"
                     /translation="MSFDVDRLRLDFPILGREVNGAPLVYLDNAATSQKPEAVLHAID
                     AYYRTCNANVHRAAHALSDEATRAFEDARERVRQFINATSTSEIVFTRGTTEAINLVA
                     SCFTQRLTAGDEILISHLEHHSNIVPWQMLAQRTGARLVAAEITESGDIDLEDFRAKL
                     SERTQMVAIGHVSNALGTINPVRQIVELARSAGAATLIDGAQAVAHLPVDVRALDCDF
                     YAFSGHKMFGPTGTGVLFGRESLLDAMPPWQGGGEMIEHVSLEESTYNRLPYKFEAGT
                     PNIAGAIGLSAAVDYLSNVPRDDARLHEDHLVDLTLSQLRQMDGVRLIGEPAERSSVV
                     SFLVDNSHPHDIGTLLDQQGVAVRTGHHCAMPLMQRLGIPGTIRASLGLYNSEADVER
                     FVAALRKALSFV"
     gene            complement(4416..5549)
                     /locus_tag="AC16QF_13655"
     CDS             complement(4416..5549)
                     /locus_tag="AC16QF_13655"
                     /inference="COORDINATES: protein motif:HMM:NF013614.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufB/SufD family protein"
                     /protein_id="HIR6449654.1"
                     /translation="MSDATAQMRDLAGTAPSWFDAQAGLARLDRGLTAPAIREYWKYT
                     PIQPFVTALVEAAHADGPAPEIHNADQPGITVCPMGELAAHQAVTVAAMAGEIDPDRY
                     PLADLALLNGSVGTFCHVAESLPQPLILKSGSNANQATFIHVAAGAEVELQESAPTGP
                     SNGQLIVVRVEAGGRVRHARCALDRLAAHWSLLVAILADNAAYELNQHHFGGRRRRSD
                     CHFVLAGPGATANVTAAFLADEGSHIDQQFVVEHRSRDTVSRQKVHGIGTGRSRCVFN
                     GRIHIHEGATASDATLTNRNLSLHPQAEIDTKPELEIYTDDVRCAHGATVGQLSEESL
                     FYLRSRGVPEAQARLLLCNGFLRECIKGPFADAVMARIREALQ"
     gene            complement(5546..>5882)
                     /gene="sufC"
                     /locus_tag="AC16QF_13660"
     CDS             complement(5546..>5882)
                     /gene="sufC"
                     /locus_tag="AC16QF_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580513.1"
                     /note="part of SUF system involved in inserting
                     iron-sulfur clusters into proteins; in Escherichia coli
                     this protein forms a complex with SufBD; the SufBCD
                     complex stimulates the cysteine desulfurase SufS in
                     conjunction with SufE; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIR6449655.1"
                     /translation="EFLKRGVNEGFSGGEKKRNEILQLLLLQPRLAVLDETDSGLDID
                     ALQVVAAGVNRFSAPGNAVLLITHYQRLLNYIVPDAVHVLSQGQIVRSGGKELALELE
                     EKGYGWITE"
BASE COUNT          971 a         1795 c         1968 g         1148 t
ORIGIN      
        1 caacgccgtc cattgcggaa cccgcccagg gctggttcga cgtcgcgata tacgagatga
       61 aggaaaccat cggcccggtg gtggagcagt atggcagcga aacgctcaag cagatcttcc
      121 actggtacta cgacttcggc gatctggtgg acgcggacgg ctatcgcgag gcgatcgccg
      181 ctcgcaccac gttgacgcgg cgctggaacc tgtttctgga cgagtacccg ctcgttctca
      241 gcccgtttct catgcggcca acctatccgt ggaactacga tgcggtcagc tatgagcagg
      301 cgcacgatct cttcaactcg ggcatctaca gcttcggcat caactatctg gggctccccg
      361 cgggggttgt tcccgttggt ctggtggaag gtctgccggc gggggtgcag atcgttggtc
      421 ggcgtttccg ggaagatctg attctcgatg ccatggaggc cgtggaggcc cgggttggcg
      481 tgctggccca cgagctgtgg cggtccgagg ccccagccgc cagctgatac ccggatgcga
      541 agcatcagcg ttctgatacc cgtcctgaac gacgcagccg ccttgcgccg attgctcgac
      601 cgcctggagg ccctggtttc ggagtcgatc atcgggtgcg agatcgtcgt gatcgatggc
      661 ggcagcaccg acggctcgca gaacgtctgc gccgagcgcg attgcctcgt cattcaatcc
      721 gcgggcggca ggggccggca actgcaagcg ggtttcgagg cgagctccgg cgatctgatc
      781 tggatgctgc atgccgacac tgatccggat tctgaagcgc ttaaatacct tgttaccctc
      841 gatgctccgg cgtgggggcg ctttgacgtc cggtttgcgg acgggccgcc ctttctctca
      901 gtggtggcga cgttgatgaa ctggcgctcc cggatgaccg gtatagccac tggggatcag
      961 gccatcttcg cccaccgctc gttgctcgaa gcgattggcg gagtgccacg ccaggcgctg
     1021 atggaggaca tcgagctgtc gaaacgtctc aaaacccgta tgcgcccgct ctgctcgcgc
     1081 catatcgtgg agacctcggg gcgtcgctgg gaagcccgcg gcgtgatccg caccatattg
     1141 agcatgtggc ggtttcgtct gcgctactgg atcggagccg atcccgagcg cctggccgcc
     1201 gagtactact cgtgagccat ctgggaacgt tgtgcatctt cgcgcgcgcg ccggagcgag
     1261 gcgctgtgaa gacgcgcctg gctgcgcgaa tcggtgccga tgctgcgttg gccgcctatc
     1321 agcgcctcgt tgaccttgca ctctcccgct acgctgcact ccccgatctc cgctccgagc
     1381 tctgggtaag cggcgatcgt tgcttgccgg aggtgcgcgg gtggtcggcg gactggggcc
     1441 tgccgttgtt cgagcagcgt ggtggcgatc tgggagagcg catggccgca gccatggccc
     1501 acggctgtgc ccgggacgtt gcaacgctga tcgttggaat cgactgtcca tgcattgacg
     1561 gcgcctatgt gcgcgctgcg gcccaaacgc ttctcacgac ggatctcgtt ctggcacccg
     1621 cggaggacgg cggttacggg ctgatcgggc tgcggcggcc aatgcccgag ctgttccggg
     1681 gcgtggcgtg gggcagcagc agcgtcctcg ccgaaacgct ggtgaaagcc gagaggctgc
     1741 ggcttcgata cgagctgctc gaaaccgtct gggacgtgga tgacgcggcg gactgggagc
     1801 gatttctcga gtccggctac gggtgaatct tggggagccg ggcgatgcgt gcttcgatca
     1861 ggcgcctgcc gcgttctcgg cagcgatgcc tctgatcctg ccgaccatcg cacgcaaccc
     1921 gtttccccgg ctggggctga gatgctggag cagctccagc tcctcgaaga gggcttccac
     1981 gtcgaatgcc agaatctcgg cgggcgagcg tttgtcgaaa gcagcaagga tgatcgcgat
     2041 cagtccccgg acgatgtgcg cgtcactgtc gatggcgaag gtgagcctgt cggcaaccgg
     2101 gtcgtaggcg gctaccagcc acacctggct ctggcagccg cgcaccaggt tgtcctcggt
     2161 cttcagggcg gcgtccagcg gtggaaggtc acgcccgatc tcgatgacga agcggtattt
     2221 gtcctcccag gaatcgaaaa acgcaaagct gtcgcggatg tcgccgatgt ccatggatca
     2281 gaacaggccg agctccagct gggcttccgc ggtcatcatg tcccgggacc agggcggatc
     2341 gaagacgagc tccacgtcca cggattcgac gtagggaacc cgtccgaccc tggatttgat
     2401 gtcctcgacc agcacgggtc ccatcccgca acccggtgcg gtgagggtca tggcgatgtg
     2461 aacgtgccgg tcggtcagcc tgcaatcgta aatcagcccg agatcgacga cgttgacggg
     2521 gatctccgga tcgtaaaccg ttgccaaggc ggccttgacc tgctccatgt cgaccttgcc
     2581 atccgcagga ggctcgaatt cgagggtctg gggctcgaac cccaaagcgt cagcatcggc
     2641 actgtccaca cgagccatgt tgccgttcat gatcacggtg tacgtcccgc cgagattctg
     2701 ggtcaacgtc acaaaggtgt tggatgggat cgtgatggga tcacctgtcg ggacgagcct
     2761 tgccgggcac tccctggtgg tggcgatgat gcgcttttcc accggccggt cgctctctag
     2821 gaacctgcga tggagaagct ttccccgcaa ccgcagtgag actcggcgtt ggggttcttg
     2881 aacttgaggc tcgagttcag gccctcggtc acgtaatcga cctccgtgcc gctcaccatg
     2941 ggcaggtcgt cggtgctcac gtagaggctc agagaatcgg caacctcgaa cgcccggtcg
     3001 ttctctgcag gctcgtccag gtaatccaga gtgtacatgt agccgttgca gccgctttcc
     3061 ttgacgccca gccgcagata tttgtggccg gacgtggcga tctggctccg gatgtgggca
     3121 gccgcggcgg gcgtcaccgc gatctgggtg ggttcgaagg tgcgaacgct catgattctc
     3181 ctcttgctcc tcctcttgct tgggtcacac gaaagacaga gctttgcgca acgcggcaac
     3241 gaatcgttcc acgtcggctt ccgaattata cagacccaga gaagctcgga tggtgccggg
     3301 aatccccaga cgctgcatca acggcatggc gcaatggtga cccgtacgca cggcgacacc
     3361 ctgctggtcg agcagcgtgc cgatgtcgtg cggatggctg ttgtccacca ggaaggacac
     3421 caccgacgat cgttcagcgg gctcgccgat gagccggacg ccgtccatct gccgcagctg
     3481 gctgagcgtc agatcgacca ggtgatcctc gtgaaggcgg gcgtcatccc gtggcacgtt
     3541 gctcaggtag tccacggcgg ccgagaggcc gatggcgccg gcgatgttgg gggtgcctgc
     3601 ttcgaacttg tagggcagcc ggttgtaggt gctctcttcg agggagacgt gctcgatcat
     3661 ctcgccgcct ccctgccatg ggggcatcgc atcgagcagc gattcgcgtc cgaagagcac
     3721 gccggtgccg gtggggccga acatcttgtg gcctgagaac gcgtaaaagt cgcaatccag
     3781 cgcgcggacg tccaccggca ggtgggcgac tgcctgggcg ccgtcgatca gcgttgcggc
     3841 gcccgcgctc ctggcaagct cgacgatctg cctgacgggg ttaattgtcc cgagcgcgtt
     3901 ggagacgtgt cctatggcga ccatctgcgt acgttcgctg agttttgccc gaaagtcctc
     3961 gaggtcgatg tctccggatt cggtgatctc cgcggccacg aggcgcgcgc ccgtccgctg
     4021 cgcgagcatc tgccagggga cgatgttgga gtggtgctcg aggtgagaga tcagaatctc
     4081 atcgccggcg gtcaagcgtt gggtgaagca gctggccacg aggttgatgg cttcggtggt
     4141 gccccgtgtg aagacgatct cgctggtcga cgtcgcgttg atgaactgac gcacgcgttc
     4201 ccgtgcatcc tcgaacgctc gggttgcctc gtcggagagc gcgtgcgcgg ctcggtggac
     4261 gtttgcgttg caggtccgat agtaggcgtc gatggcatgg agcacggctt ccggcttctg
     4321 ggaagtcgcg gcattgtcga gatacaccaa aggtgccccg ttgacctcgc gtccgaggat
     4381 gggaaagtcc agccgcagtc ggtcgacgtc gaagctcatt ggagggcttc ccggatccgc
     4441 gccatgacgg catcagcgaa tggccccttg atgcactccc ggaggaatcc gttgcacagt
     4501 agcagccgtg cctgggcctc cggaacgccg cgcgagcgaa ggtagaagag gctttcctcg
     4561 gagagctgcc cgaccgttgc gccgtgggcg cagcgaacgt cgtcggtgta gatctcgagc
     4621 tcgggcttgg tgtcgatctc ggcctgaggg tgcagtgaga ggtttcgatt ggtcagggtg
     4681 gcatcgcttg cggtggcgcc ctcgtgaatg tggatcctgc cgttgaacac gcaacggctg
     4741 cggccggttc cgatgccgtg gaccttttgt cggctcacgg tgtcgcgcga gcggtgctcg
     4801 accacgaact gctgatcgat gtggctgccc tcgtcggcga ggaaagcggc cgttacgttc
     4861 gctgtcgctc cgggcccggc cagcacgaaa tggcaatcgc tgcgccggcg gcgaccgccg
     4921 aagtgatgct ggttcagctc gtaggctgca ttatcggcca ggatcgcgac gagcagtgac
     4981 caatgcgccg ccaggcggtc cagggcgcaa cgtgcatgcc ggacccgtcc gccggcctct
     5041 acccgcacga cgatgagctg gccgttgctc ggcccggtcg gtgcgctttc ctgcagctcg
     5101 acctcggcgc ccgccgccac gtggatgaac gtggcctgat tggcgttact acccgatttt
     5161 agaatcagag gctgcggcag gctttctgcg acgtggcaga aggttccgac gctgccattg
     5221 agtagggcaa gatccgcgag aggataacga tcgggatcga tctcccccgc catggcggca
     5281 acggtgacag cctggtgcgc ggccagctcg cccatagggc agaccgtgat gcccggctga
     5341 tcggcattgt gaatctcggg tgcggggcca tcggcatgcg ctgcctcgac gagggccgtc
     5401 acgaacggct ggatgggcgt gtacttccag tattccctta tcgcgggggc agtcagccct
     5461 cgatccaggc gcgcgagccc ggcttgcgcg tcgaaccagg acggtgcggt accggccaga
     5521 tccctcattt gcgcggtcgc gtcgctcatt cggtgatcca tccgtatccc ttctcttcga
     5581 gttccagcgc cagctccttt ccgcccgacc tcacgatctg gccctgtgac agaacgtgca
     5641 cggcgtcggg cacgatgtag ttgagaagcc tctggtagtg cgtgatgagc agcacggcgt
     5701 tgcccggcgc gctgaaacgg ttcacgcctg cggcgacgac ctgaagcgcg tcgatgtcca
     5761 gccccgaatc ggtctcgtcg aggaccgcca gccttggttg gagcagcagc agctgaagga
     5821 tctcgttgcg cttcttctcg ccacccgaga aaccctcgtt aacgccacgc ttcaagaatt
     5881 cg
//
LOCUS       DBNHLM010000294         5962 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_999970, whole genome shotgun sequence.
ACCESSION   DBNHLM010000294 DBNHLM010000000
VERSION     DBNHLM010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_999970"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="AC16QF_13665"
     CDS             <1..807
                     /locus_tag="AC16QF_13665"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF044908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6449656.1"
                     /translation="NHQTEHVFGGQVLAQAIAAAYRTVDVPHGLHSIHAYFLRPGDWR
                     VPILYEVDRIRDGRSFSTRRVVAVQNGRAIFSMASSWQKDEEGLEHAVQMPDVPPPGA
                     LRGDREAYLEAAREHPEISRFAFRFEAIDSRQVERILMTDSGTHPPYKHTWLRTRETL
                     PDDPEIHLAMLAYMSDLDFMSTSMLPHGRNTIREHVQGASLDHSIWFHRPFRADEWLL
                     FAKESPNAGGARGFVRGRFFNRDGELVASAAQECLIRPRGALAENLASPA"
     gene            complement(817..1296)
                     /locus_tag="AC16QF_13670"
     CDS             complement(817..1296)
                     /locus_tag="AC16QF_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HIR6449657.1"
                     /translation="MAIQEGDKLPEATMHIIQDGRPTPVSTGDLFAGKKVVVFAVPGA
                     FTPTCSQAHLPGYVVNADAIKAKGVDSIVCLSVNDAFVMDCWGQDRNAEEILMVGDGN
                     GDFTRAIGLEMDGSGFGLGTRSQRYAMIVEDGTVTKLALEEPGQFEVSKAEAILEAL"
     gene            complement(1388..2047)
                     /locus_tag="AC16QF_13675"
     CDS             complement(1388..2047)
                     /locus_tag="AC16QF_13675"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6449658.1"
                     /translation="MECDERLLELISENLARFEASDADVDDAHHAAVAITVTDVGPGA
                     DLPGLSGTDGWQSQAGLLLTRRSAQLRKHAGQWAFPGGRLDPGETPAETALREMHEEV
                     RVDLGPDRILGRLDDFVTRSGFVMTPFVVWGGAGLQASPNPDEVQSIHRIPLTEFLRE
                     DAPVLEDADSSDRPVLKMPVGTEHIAAPTAALIYQFREVCLLGRHTRVAHYEQPAFAW
                     R"
     gene            2156..3040
                     /locus_tag="AC16QF_13680"
     CDS             2156..3040
                     /locus_tag="AC16QF_13680"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6449659.1"
                     /translation="MSHDSERQALVAALAAVLLWSTVATGFKLGLAVMAPLQLLLAGT
                     TVSAIFFAAVTTARRSWSVTGRQLGEAAAFGIVNPALYYLVLFEAYDRLPAQLAQPLN
                     YTWAITLAVLAVPILKQRLSARTLAGILVSYLGVLVLLSQGRFDRLPAVDWTGVMLAL
                     GSTLLWAGYWLMNARSRSDSVNLMALSFLIATPLVAIACVLGPGLPTLDWNVAWFGAW
                     VGLVEMGVTFLLWQRAMGLTSHAARIGQLIFLSPFISLMLISTVLGETIHWTSVAGLV
                     VIVGGVLLTRSAAPGRAR"
     gene            3108..3704
                     /locus_tag="AC16QF_13685"
     CDS             3108..3704
                     /locus_tag="AC16QF_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6449660.1"
                     /translation="MIRTTTAVVTALLALAAAPNLTASESDTIARGQYLVTLLGCGRC
                     HTEGYLTGDKATGPYLGGSRVGIAYTAYSMDDTHPGVVFAGNLTPDDATGLGTWSEEE
                     IVQAMTVGVAKSGHERLTVMPWTNYGALKEKDVRAIARYLKSLSPVRWPIPEAIPEGD
                     PIDEPYVRFGVYKFYPHGPVEAINLPVPTNEPSPEGVQ"
     gene            complement(3775..4563)
                     /locus_tag="AC16QF_13690"
     CDS             complement(3775..4563)
                     /locus_tag="AC16QF_13690"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6449661.1"
                     /translation="MSYETLLFEVADGVGTITINRPEAFNAMSPLCAKEFADVAVRCD
                     DDPDVRAVVLTGAGKAFCAGGDLGAFASAGSSAKSLLKVMAGDLHMGLSRLARGNAPV
                     IAAVNGTAAGAGFSLVMAADLAIAAHSAVFTMAYTNAGLSPDGSSTFYMPRKIGDRRT
                     RELMLTNRLLKAEEALAWGVVNQVVADDQVVAEAMKLAQKLAAGPTLAFGAVKTLLNG
                     TFDQTLESQMELEARAIAELSVTADGQEGINAFMAKRKPEFRGA"
     gene            complement(4586..5188)
                     /locus_tag="AC16QF_13695"
     CDS             complement(4586..5188)
                     /locus_tag="AC16QF_13695"
                     /inference="COORDINATES: protein motif:HMM:NF036525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6449662.1"
                     /translation="MANERITRATTPARRASVLDAALACFSERGLEGTTVSDIQSAAG
                     CSVGSIYHHFGSKEGIAEELFILGVQRLNQRMLKRLRRCKTGEESVRAVVGEYVRWAT
                     RESAMARYLHSRDVSFSADARERLRDIHREYVEALFAWFAPFVETGEMRRLPVQVYIP
                     LISGPIQEYVRRWLSGQHTRSPQQVEALFADAAWNAVKAR"
     gene            5274..>5962
                     /locus_tag="AC16QF_13700"
     CDS             5274..>5962
                     /locus_tag="AC16QF_13700"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449663.1"
                     /translation="MDIELSTEDVEFRAEVRAFLKDNAYEAGTDYNEWRNGWFEKAAE
                     KGGWDVPKWPEAFGGPGWSPTQHYIWEQETAVATLPWDLPFGIGMLAPILMSYGNEEQ
                     QKRFLPDIRARKVNWCQGYSEPGAGSDLASLKTKAVLSEDGSHYTVNGTKIWTTVAHI
                     ADWIFCLTRTDDSGKKQEGITFLLFPMKQPGVEVKPIITLGGAHSVNTVFLTDVKVPV
                     ENRIGEEGKGWT"
BASE COUNT          994 a         2046 c         1889 g         1033 t
ORIGIN      
        1 aaccatcaga ccgagcacgt tttcggcggc caggtgctgg ctcaggccat cgccgccgcg
       61 taccgcaccg tggacgtacc ccatggcctg cactccatcc acgcctattt cctccgcccc
      121 ggtgactggc gggtgccgat cctgtacgag gtggaccgga tccgggacgg ccgcagcttc
      181 tccacgcgcc gggtggtggc cgtccagaac ggacgggcca tcttcagcat ggcgtcctcg
      241 tggcagaaag acgaggaagg actagagcac gccgtgcaga tgcccgacgt gccgccgccc
      301 ggcgctctgc gcggagaccg tgaggcgtac ctggaagccg cgcgcgagca cccggaaatc
      361 agccgtttcg cctttcgttt cgaagccatc gacagcaggc aggtggagcg gatcctgatg
      421 accgactcgg gaacccaccc gccgtacaag cacacctggc tgcggacccg cgagaccttg
      481 cccgacgatc ccgagatcca tctcgccatg ctcgcctaca tgtccgacct ggacttcatg
      541 agcacgtcca tgctgcccca tgggcgcaac acgatccgcg agcacgttca gggagcgagc
      601 ctggatcact cgatctggtt tcaccggccg ttccgggccg acgagtggct gctgttcgca
      661 aaggagtcgc ccaacgcggg cggcgcgaga ggtttcgtgc gcgggcggtt cttcaaccgg
      721 gatggcgaac tcgtggcgag cgccgcacag gagtgcctga tccgaccgcg gggcgcgctg
      781 gccgaaaacc tcgcgagccc cgcgtgaccg gcagacctag agcgcttcta gtatcgcctc
      841 ggccttgctc acctcgaact gcccgggctc ttcgagcgcg agcttggtga cggtgccgtc
      901 ctccacgatc atcgcgtagc gctgggatcg cgttcccaga ccgaaaccgc tgccgtccat
      961 ctcgagaccg atggcacggg taaagtcccc gttgccatca ccgaccatca ggatttcctc
     1021 agcgttgcgg tcctggcccc agcagtccat gacgaacgcg tcgttcacgg acaggcacac
     1081 gatggagtcg acgcctttgg ctttgatggc gtccgcattg accacgtagc ccggcaggtg
     1141 agcctgggag cacgtgggcg tgaacgcgcc cggtacggca aagaccacca ccttcttgcc
     1201 ggcgaaaaga tcgccggtgc tgaccggcgt cgggcgcccg tcctggatga tgtgcatggt
     1261 ggcttctggg agcttgtcgc cttcttgaat cgccatggcc ctttaatgtc cttcttctgt
     1321 tgagcgggtc cgaaagtttg gggaaagacg cttcgatggt caacgaacgc tgatcgatcc
     1381 gggcggatca gcgccaggca aaggccggct gctcgtagtg ggcgacccga gtgtgccggc
     1441 cgagcaggca gacctcccgg aactggtaga tcagcgccgc ggtgggcgct gcgatgtgct
     1501 cggttcccac cggcatcttc agcacgggac ggtcgctgct gtcggcatcc tccagcaccg
     1561 gcgcgtcctc ccgcagaaac tcggtgagcg gtatgcggtg gatggactgc acctcgtcag
     1621 gattcggcga tgcctgaaga cccgccccgc cccagacgac gaagggcgtc atcacgaacc
     1681 cggagcgcgt cacgaagtcg tccagacgac cgaggatccg gtcggggcca agatcgaccc
     1741 gcacctcctc gtgcatctcc cgcagcgcgg tctccgccgg cgtctcgccg gggtcgagcc
     1801 gcccgccggg aaacgcccac tggccggcat gcttcctgag ttgcgccgag cggcgggtga
     1861 gcagcaggcc tgcctgggat tgccatccat cggtgcctga aagacccggc agatcggcac
     1921 cggggccgac gtcggtgacg gtaatggcca ccgcggcgtg gtgggcgtcg tccacgtccg
     1981 catcgctggc ttcgaagcgg gccaggttct cgctgatcag ttccagcagg cgctcatcgc
     2041 attccatcga agcagccgtc aatttgggcg gtgtgtggcg agcccccttc ggggcccgtt
     2101 cgcgaatcat gcgccctggt gtacgctgac gcaatttcac gccccggcga ccccattgag
     2161 ccacgactcg gaaaggcagg cactcgtcgc cgcgctcgcg gccgtgctgc tgtggtccac
     2221 ggtcgccacc ggcttcaagc tggggcttgc ggtgatggcg ccgctgcagc tgctgctggc
     2281 cggaaccacg gtctccgcca tcttcttcgc cgccgtcacc accgcgcgcc gcagctggtc
     2341 ggtgaccggc cgccagctgg gggaagcggc cgcgttcggc atcgtcaatc ccgcccttta
     2401 ctacctggtt ctgttcgagg cctacgaccg gctgccggcc caactggccc aacccctgaa
     2461 ctacacctgg gccatcaccc tggcggtgct ggccgtcccc atcctgaagc agcgtctctc
     2521 ggcgcgcacc ctggccggta tcctcgtgag ctatctgggg gtgctcgtcc tgctctccca
     2581 agggcgcttc gaccgcctgc ccgccgtgga ctggaccggc gtgatgctgg ccctgggcag
     2641 cacgctgctg tgggccggct actggctgat gaacgcccgt tcccggagcg attccgttaa
     2701 cctgatggct ttgagctttc tgatcgcgac tcccctggta gccatcgcct gcgtgctggg
     2761 ccccggcctg ccgaccctgg actggaacgt cgcgtggttc ggcgcgtggg tgggtctggt
     2821 ggagatgggg gtgacttttc ttctgtggca gcgggccatg gggctgacct cccacgcggc
     2881 tcgcatcggc cagctcatct ttctctcgcc cttcatttcc ctgatgctca tctcgacagt
     2941 gctgggtgaa accatccact ggacgtccgt cgccggcctc gtcgtcatcg tcggcggcgt
     3001 gctcctgacc cgatctgcgg cgcccgggcg ggctaggtga ttcccgcggt atcgcgacgc
     3061 cgccaagtcg ggcaccatcg gcccactgtc acaagtggat gcaacccgtg atcaggacga
     3121 ctaccgcggt ggtgaccgcc ctcctcgcac tggccgccgc cccgaacctc acggcctcgg
     3181 agtcggacac cattgccaga ggccagtacc tggtcaccct gctgggctgc gggcggtgtc
     3241 ataccgaggg ctacctcacc ggcgataagg ccacgggtcc ctatctcggc ggttcccgcg
     3301 tgggcatcgc gtacacggcg tacagcatgg acgacaccca tccaggggtg gtcttcgccg
     3361 gcaacctgac ccccgacgac gcaaccggtc tgggaacctg gagcgaagag gagatcgtcc
     3421 aggccatgac cgtgggcgta gccaagagcg gtcacgagcg cctgaccgtg atgccgtgga
     3481 cgaactacgg ggcgctgaag gaaaaggacg tccgagccat cgcccgctat ctgaagagcc
     3541 tctcccccgt gcgctggccg atccccgagg ccattcccga gggagatccg atcgacgagc
     3601 cctacgtacg cttcggcgtc tacaagttct acccccacgg cccggtggag gccatcaacc
     3661 tgccggttcc caccaacgag ccgtcgcccg aaggggtgca atgaccgcaa caagccgcgg
     3721 gctttcgtgc ggctccaggg ccgacggggt tgcagattcc gggcgcgccg cgatctaggc
     3781 gccgcggaac tccggcttgc gcttggccat gaaggcgttg atgccctcct ggccgtcggc
     3841 tgtcacggat agctcggcga tcgctcgggc ttccagctcc atctgcgatt cgagggtctg
     3901 atcgaacgtg ccgttgagca gggttttcac cgcaccgaac gcaagcgtcg gccccgccgc
     3961 cagtttctgc gcgagcttca tggcctcggc aaccacctga tcgtccgcca caacctggtt
     4021 gacgaccccc caggccaagg cctcctcggc tttcagcagc cggttggtga gcatgagctc
     4081 tcgcgttcgc cggtcgccga ttttccgcgg catgtagaaa gtcgagctgc cgtcgggaga
     4141 aaggccggca ttggtgtagg ccatggtgaa caccgcgctg tgtgcagcga tggccagatc
     4201 ggcggccatg accaggctga atcccgcccc cgccgccgtg ccgttcacgg ccgcgatgac
     4261 gggcgcgttg ccccgggcaa gccgcgagag ccccatgtgc aggtccccgg ccatcacctt
     4321 gagcaggctc ttcgcgctcg acccggcgct ggcgaaggcg cccaggtccc caccggcgca
     4381 gaacgctttg cccgctcccg tgagcaccac cgcgcgcacg tcgggatcgt cgtcgcagcg
     4441 cacggccacg tcagcaaact ccttcgcgca cagcgggctc atggcgttga atgcctcggg
     4501 ccggttgatg gtgatcgttc ccacgccgtc ggccacttcg aacagcaacg tctcgtaact
     4561 catctctacg ccttaacccg tgcggttatc gagcttttac cgcattccag gccgcatccg
     4621 caaacagcgc ctccacctgc tgcggcgagc gggtgtgctg cccggagagc cagcgtcgca
     4681 cgtactcctg gatgggaccg ctgatgaggg ggatgtaaac ctggaccggc aggcggcgca
     4741 tctcgccggt ttccacgaac ggcgcaaacc aggcgaacag ggcctccaca tactcccggt
     4801 ggatgtcccg cagccgctcc cttgcgtccg ccgagaagct gacgtcgcgt gagtgcagat
     4861 agcgggccat cgcagactcc cgcgtggccc agcggacata ctcgcccacc accgcgcgta
     4921 cgctttcctc cccggtcttg caccgccgca ggcgcttgag catgcgctgg ttgagacgct
     4981 gcacgccgag gatgaacagc tcctccgcga tgccttcctt gctgccgaaa tgatgataga
     5041 tgctgcccac actgcagccg gcggcgctct ggatgtcgct gaccgtggtc ccctcgagac
     5101 cgcgttcgga aaagcaggcg agcgccgcat ccaggacgct cgcgcgccgc gccggggtag
     5161 tcgctcgggt gattctctcg ttggccatgg cgtcatggta actagaattc agttctagta
     5221 aaagattcta atcagcgtta atatccggcc cttctcgaat ggaggattca gccatggaca
     5281 tcgagctctc gacggaggac gtcgagttcc gcgcggaggt ccgcgcgttc ctgaaagaca
     5341 acgcctacga agccggcacg gattacaacg agtggcgcaa cggctggttc gagaaagccg
     5401 ccgagaaagg cggctgggac gtgcccaagt ggccggaggc cttcggcgga ccgggatggt
     5461 cacccaccca gcactacatc tgggaacagg aaaccgcggt ggccaccctg ccctgggacc
     5521 tgcccttcgg catcggcatg ctggctccca ttctcatgtc ctacggcaac gaggagcagc
     5581 agaagcggtt tctgcccgac atccgggccc gtaaggtcaa ctggtgccag ggctactcgg
     5641 agcccggggc cggctccgac ctagcctcgc tcaagaccaa ggcggttctg tcagaggacg
     5701 gcagccacta caccgtcaac ggcaccaaga tctggaccac cgtagcccac atcgccgact
     5761 ggatcttctg cctgacccgc accgacgatt ccggcaagaa gcaggaggga atcaccttcc
     5821 tgcttttccc catgaagcag cccggcgtag aggtcaaacc catcatcacc ctcggaggtg
     5881 cccacagcgt caacacggtg ttcctcaccg acgtgaaggt tcccgtggaa aaccgcatcg
     5941 gcgaggaggg caagggctgg ac
//
LOCUS       DBNHLM010000295         4885 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_760389, whole genome shotgun sequence.
ACCESSION   DBNHLM010000295 DBNHLM010000000
VERSION     DBNHLM010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_760389"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="AC16QF_13705"
     CDS             complement(<1..1283)
                     /locus_tag="AC16QF_13705"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6449664.1"
                     /translation="MAAVVKPGVRTMDPSFAKLLDSDTNAAHVTRAMRRDSPIQPGPT
                     LVPTARYISREYHELEKQRLWSRVWQMAAHEDDFPNVGDVVPYDIVDRSYLVVRVADD
                     EYKAYYNACLHRGRKLRESRARGLDELKCPFHGWAWELDGTLKQVPCAYDYTDLDREA
                     ERLPEVRIGRWGRFIFVNPDPDCEPLEDFLGDLSSQFEFFPYERRYKQAHVAKIIRAN
                     WKVVQEAFMESYHVYMTHPQALAHGVHDGDTKYDVFGNYSRAILCGALDGSGVPDWGP
                     PPEQNEGITRVRHPLTGTIYESAGDGVVNVTTRDGRSGRFDALANWLDGDLIDANPHM
                     CIVVAGVQLPDIGVAGVETTPEDAARAMERLGDDATGRALMSEMQREVLRRVIPSVAD
                     DVPDIELTAAIYLTVFPNYHPWGSFNRINYRFRPNG"
     gene            1416..2654
                     /locus_tag="AC16QF_13710"
     CDS             1416..2654
                     /locus_tag="AC16QF_13710"
                     /inference="COORDINATES: protein motif:HMM:NF012503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR6449665.1"
                     /translation="MRTRLPESGSDWPTIKAEMVQLGRGDAKWRDGKTAVYVFNAGDD
                     VAQVQREAYALYMSENGLGPLAFPSLKRMEDEVIDMALSILHAPEDAAGAMTSGGTDS
                     ITMAVKAARNHARAQRGMNAPGNLVIPYSAHPAFDKAAHLMDVQVRRVPVGEDLLADV
                     PAMANAIDRETIMLVGSAPCFPYGLIDDIAALSAVALERGLWLHVDACVGGFIAPFVR
                     MNGSDVPPFDFELPGVASMSADLHKYGYCAKGASTVMFRSDGLYQNMPFDFGDWPGGR
                     MVTPTLAGTRPGGAIAAAWAVMNFLGRTGYQTKHRQVTNARQAVERGVRQLGFEVLGS
                     PKLGIVAFSHAQADVIAVYRHMYRKGWFAGLTTEPKALHLMLSPVHEGVTDLYLTDLA
                     DSLRLAEGQESDDGFEARYS"
     gene            2698..2835
                     /locus_tag="AC16QF_13715"
     CDS             2698..2835
                     /locus_tag="AC16QF_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449666.1"
                     /translation="MWAIAITGFAPGSISGLLDGTFVDSQAVHVHAAVCGAHHDAATI
                     D"
     gene            complement(2981..3421)
                     /locus_tag="AC16QF_13720"
     CDS             complement(2981..3421)
                     /locus_tag="AC16QF_13720"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6449667.1"
                     /translation="MKVESLGHLVLKVRDLDRSEQFYSGVLGMPVCARFDDKGLKMTF
                     FSLGNHHDLAIMQVSGEEASAGEQAVGLHHVAFCIGRSMDQLREAKAQLEAAGVETDP
                     IDHDVTKSLYFDDPDGHNIELYVDVSDAWKKEPQRIAVLEPLEL"
     gene            complement(3501..4073)
                     /locus_tag="AC16QF_13725"
     CDS             complement(3501..4073)
                     /locus_tag="AC16QF_13725"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6449668.1"
                     /translation="MSEAEGLGARVAALEEELGRLTDLQAIQDVLVRYSRALDWLDDA
                     MLETVFYDDADIDYGFFRGNGKDFKKALMELEHGIGRRWHFTSQLEIDLNGDSADVES
                     YNLSLALPEVEPSDGGGYSVFFGFYKDRLVKRGGKWGIIARKHIQVSGAPLAELAMEG
                     DMGLLNQIGLTSPSHPDYRRLTNAQPLDDS"
     gene            complement(4108..>4885)
                     /locus_tag="AC16QF_13730"
     CDS             complement(4108..>4885)
                     /locus_tag="AC16QF_13730"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6449669.1"
                     /translation="RRYGDSDHLLMCVSGALQTMAIWRTVVKRFADRYTVVIFDMPGI
                     GRSEIMSGGAHVTVQEQLETVHALIQETHPGGEVTLAGSSWGTAIAAAYAALRPDEVQ
                     HLILSSFGMKANAGMEEIVRRALALYEGGDYARGADLILEMFGGQIGETYRRQIIAQF
                     AQLNDAHAEAFAEHCRNIVKLGNLDDIIDLTRIKARTFIINGANDTIVDLEDMYVAQR
                     LIPDCQVRLIEGVGHFLHFERPELLDDYAEFMTGSAVLTS"
BASE COUNT          874 a         1587 c         1491 g          933 t
ORIGIN      
        1 ccgttcggtc gaaagcggta gttgatgcgg ttgaaagaac cccacggatg atagttcggg
       61 aacacggtga gatagatggc cgcggtcagc tcgatgtcgg gaacgtcgtc ggctacggag
      121 ggtatgaccc tgcgtagcac ctcccgctgc atttcgctca tgagcgcacg acccgtggcg
      181 tcgtccccca gtcgctccat ggcccgggcc gcatcctccg gcgtggtctc cacgcctgca
      241 accccaatgt ccggcagctg cacgccggcc accacgatgc acatgtgcgg attcgcgtcg
      301 atcagatcgc catcgagcca gttggcaagc gcatcgaatc ggcccgagcg gccgtcgcgg
      361 gtcgtgacgt tcaccacgcc gtcaccggcg gactcgtaga tcgtccccgt gagcggatgt
      421 cgaaccctgg tgatcccttc gttctgctcg ggcggcggcc cccaatcggg aacccctgat
      481 ccgtccaggg ctccgcagag gatggcccgc gaatagttgc cgaagacatc gtatttggta
      541 tcgccgtcat gaacaccatg agccagcgcc tgggggtgcg tcatgtagac gtgatacgac
      601 tccatgaagg cctcctgaac gaccttccag ttcgcgcgga tgatcttggc aacgtgggcc
      661 tgcttgtaac gacgctcgta gggaaagaac tcgaactgcg acgagaggtc gccgaggaag
      721 tcttccaggg gctcgcagtc cggatccgga ttgacgaaga tgaagcgccc ccagcgtccg
      781 atccgaacct cgggcaggcg ctccgcctcc cggtcgaggt cggtgtagtc gtaggcgcag
      841 ggaacctgct tgagcgtgcc gtcgagctcc caggcccagc catgaaacgg gcatttgagc
      901 tcatcgagcc ctcttgcccg agactctctg agcttgcggc cccggtgcag gcaggcgttg
      961 tagtaggctt tgtattcatc gtcggcgacc cgaaccacca ggtacgacct gtccacgatg
     1021 tcgtagggca cgacgtcgcc cacgttcggg aaatcgtcct cgtgggcggc catctgccac
     1081 acgcgcgacc agagacgttg cttctccagc tcgtgatact cacgggatat gtaacgtgcg
     1141 gtgggcacca gggtagggcc cggctgaatg ggcgaatcgc gccgcatggc acgggtcacg
     1201 tgagcggcgt tggtgtcgga gtcgagcaac ttcgcaaacg acgggtccat cgtccgcaca
     1261 ccgggtttaa cgactgccgc catggctctc ctcccccaat ttaccctgat cgatgcccta
     1321 atctacccca aacccgccga ccggtctgct gatagatggt aacctccggg catcagcggc
     1381 agcgccgcgc cgcgccgccg gacaggggag cacagatgag aacgcgccta cctgaatcgg
     1441 gctccgactg gccgacaatc aaggccgaga tggttcagct cggccgcggc gatgcgaagt
     1501 ggcgggacgg caagacggcc gtttacgtgt tcaacgcggg agacgacgtc gcccaggttc
     1561 aaagggaagc ctacgccctg tacatgtccg agaacggcct gggaccgctg gcatttccca
     1621 gcctgaagcg gatggaggac gaggtgatcg acatggccct gtcgatcctc cacgcacccg
     1681 aggacgctgc gggcgcgatg acgtcaggcg gaaccgattc catcaccatg gccgtcaaag
     1741 ccgcccggaa ccatgcccgg gctcagcgcg gcatgaacgc cccgggcaac ctggtgattc
     1801 catactcggc ccacccggct ttcgacaagg cggcgcacct catggacgtg caggtgcgca
     1861 gggtaccggt cggagaggat ctccttgccg acgtgcccgc catggcgaac gccatcgacc
     1921 gagagacgat catgctcgtt ggctcggccc cctgcttccc ctacgggctg atcgacgaca
     1981 tcgcagcgct ctctgccgtt gccctcgaac gcggactatg gcttcacgtg gatgcctgcg
     2041 tgggcgggtt catcgcccct ttcgtgcgga tgaacggctc ggacgttccc cctttcgatt
     2101 tcgagctgcc aggcgttgcg tccatgtcgg ccgacctgca caagtacggc tactgcgcga
     2161 aaggcgcgtc caccgtgatg tttcgcagcg acggcctcta ccagaacatg ccgttcgact
     2221 tcggcgactg gccagggggt cgcatggtga cgccgacact ggcgggcaca cgtccggggg
     2281 gcgccatcgc tgccgcctgg gccgtgatga acttcctcgg caggaccgga tatcagacaa
     2341 agcaccgcca ggtcaccaat gcccggcagg ccgtcgagcg gggggtgcgg cagctcggct
     2401 ttgaagtgct cggctcgccg aagctcggca tcgttgcctt ctcccatgct caggccgacg
     2461 tgatcgccgt gtacagacac atgtaccgca aaggctggtt cgcggggctg acgaccgagc
     2521 cgaaggctct tcacctcatg ctgtcacccg tccacgaggg ggtcaccgat ctctacctga
     2581 ccgatcttgc agacagcctc cggctcgccg aaggccagga atccgatgat ggtttcgagg
     2641 cacgatacag ctgagctcag cccgtcacgg cccttttact tctggctcag cttgtggatg
     2701 tgggccatag ccatcacggg ctttgcgccg ggatcgattt ccggtctgct ggacgggacc
     2761 ttcgtggatt cgcaggccgt gcacgttcac gccgccgtct gcggcgcgca ccatgatgct
     2821 gccaccattg attgacatgc ttgtttttcc cgtgctcttc tgggtcgcct atcgttttcg
     2881 gaatcggccc gaaacgcaca agcggctgat ggtcgtcgcc acgacgttgc tgctcgtcgc
     2941 ggcagtgggg cgcatgagct tccttggccg cccgccggct ttacagttcc agcggctcca
     3001 gcacggcgat gcgctgcggt tctttcttcc aggcgtctga gacgtccacg tagagctcga
     3061 tgttgtgccc gtccgggtca tcgaaataga gcgacttggt gacatcgtgg tctatcgggt
     3121 ccgtctcgac gcccgcggct tcgagctgcg ctttcgcttc acgcagctga tccatggacc
     3181 tgccaatgca gaaggccacg tgatggagcc ccactgcctg ttcaccggct gaagcttctt
     3241 cgccggatac ctgcatgatc gccaaatcat ggtgattgcc cagactgaag aacgtcatct
     3301 tcaatccctt gtcatcgaac cgcgcacaaa ccggcatgcc gagcacgccg gagtagaatt
     3361 gctccgagcg gtcgaggtcg cgaactttga ggacgaggtg cccgagagat tcaactttca
     3421 tcgtcagctc cagaacttcg acatcgcaac tatgccggtc ccacgcgtgc aagccaatca
     3481 cgaatcggcc aggaagccgg tcaggaatcg tccagcggct gggcgttcgt aaggcgccga
     3541 tagtcggggt gggaggggct cgtcaggccg atctggttga gcaggcccat gtccccttcc
     3601 atcgccagct ccgccaacgg ggcgccgctc acctggatgt gcttgcgcgc gatgatgccc
     3661 cacttgcccc cgcgcttgac gagccggtcc ttgtagaagc cgaagaagac cgagtaaccc
     3721 ccgccgtcgc tcggttccac ctccggaagc gcaaggctga ggttgtagct ctcgacgtcg
     3781 gccgaatcgc cgttgaggtc gatctcgagc tggctggtga aatgccagcg gcgccctatc
     3841 ccgtgctcga gctccatcag cgcctttttg aaatcctttc cgtttccgcg gaaaaagccg
     3901 tagtcgatgt ccgcgtcgtc gtagaacacg gtctccagca tggcatcgtc cagccagtcc
     3961 agcgcccggg agtagcgcac cagcacgtcc tgaatggcct gcaggtccgt aagcctccca
     4021 agttcctctt cgagcgcagc aacccgcgca cccaaccctt ctgcttcact catggtcttc
     4081 tcctggactg cggatgctcg acgggtttca gctcgtcagg acggcggagc cggtcatgaa
     4141 ctccgcatag tcgtcaagca gctccggccg ctcgaaatgt agaaagtgcc ccacgccctc
     4201 gatgaggcgc acctggcaat ccggtatcag tcgctgagcc acgtacatgt cctcgagatc
     4261 gacgattgtg tcgtttgcac cgttgatgat gaacgttcgc gccttgatcc gggtcaggtc
     4321 gatgatgtcg tccagattgc cgagcttgac aatgttgcgg caatgctcgg cgaacgcctc
     4381 cgcatgggcg tcgttgagct gagcgaactg ggcgatgatc tgacgtctgt acgtctcgcc
     4441 gatctgcccc ccgaacattt ccagaataag gtcggcgccg cgagcgtaat ctcccccctc
     4501 atacagcgcc agagcgcgcc tgacgatttc ctccataccc gcgttggcct tcatgccgaa
     4561 ggagctcaga atcaggtgct ggacctcgtc gggcctgagc gcggcatacg ccgcggcgat
     4621 ggcggtgccc catgagctcc cggcaagggt cacctcgccg cccggatgag tttcctgaat
     4681 gagcgcatgg acggtttcga gctgctcctg gaccgtgacg tgcgcaccgc cggacatgat
     4741 ctcgctgcgc ccgatccccg gcatgtcgaa gatcaccacc gtgtaacgat cggcgaaacg
     4801 tttgaccacg gttcgccaga tcgccatggt ctgcagcgcg ccgctcacgc acatcagcag
     4861 atggtcgctg tccccatacc ggcgg
//
LOCUS       DBNHLM010000296         6657 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1180081, whole genome shotgun sequence.
ACCESSION   DBNHLM010000296 DBNHLM010000000
VERSION     DBNHLM010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1180081"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="AC16QF_13735"
     CDS             complement(<1..583)
                     /locus_tag="AC16QF_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR6449670.1"
                     /translation="MASPAYGNWHSPLQAAEIASGAIALRDLRSHGDTLYWLESRPAE
                     QGRTTLMALRPGERPTELTPEPFNVRTRVHEYGGGAYLPCAEGVFFANWSDQRIYLIA
                     NGAISALTRNDQSLRFADFCWDGARERLVAVTEQHGAEGEPVNRLQAIELRNPGELTT
                     LHAGHDFYSSPTVSPDMGHLVFMCWDHPNMPWDG"
     gene            complement(588..1109)
                     /locus_tag="AC16QF_13740"
     CDS             complement(588..1109)
                     /locus_tag="AC16QF_13740"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6449671.1"
                     /translation="MAERIYIRPVSRRDRSEFLHMTQRSQHVHEPWISPPLSDATFHS
                     YLKRTEQDDHEGLLVCLTDNDDIVGVININNIVRGSFLSASLGYYVSATQMGHGYMRE
                     GLELVKTYAFCQLGLHRLEANIQPENGRSIELVRSCGFQLEGLSRSFLFIAGSWRDHE
                     RWTAFHPRATLLP"
     gene            complement(1102..1326)
                     /locus_tag="AC16QF_13745"
     CDS             complement(1102..1326)
                     /locus_tag="AC16QF_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449672.1"
                     /translation="MRYLSRFALLCMGFALTTAGLMLWHAVGFGFHGIWFFDNDYRLH
                     PLHLLIVGLAMIPPALWEIFVLESRRRSRG"
     gene            complement(1323..2249)
                     /locus_tag="AC16QF_13750"
     CDS             complement(1323..2249)
                     /locus_tag="AC16QF_13750"
                     /inference="COORDINATES: protein motif:HMM:NF024919.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIR6449673.1"
                     /translation="MILTERFADPDTGKRYPLRCGGPIGWRSAGDKHTAIIQLPPIPA
                     GAILVPSLSTAPSPERFQFVLRAASRLWALHEVPCRAAPQPSADTAVTTHVDCFHVHE
                     DLPRAELTVTVTGPSSPARYLLTVTARQLELERAPAPTRSAICKAPPFLSQMALGTSV
                     GPRVCSPACITMLLARHGHAADLKSVSESCFDAVTNLYGIWPLAIRAAAEQGSLGAVE
                     LFDDWEEPLSVLDAGMPFAASVRFGEGELPGAPLTRTAGHLVVVHGADPQDVHVNDPR
                     APEAASVPRRYPAVDFSRAWLRHRGAAYILLP"
     gene            2271..2522
                     /locus_tag="AC16QF_13755"
     CDS             2271..2522
                     /locus_tag="AC16QF_13755"
                     /inference="COORDINATES: protein motif:HMM:NF017574.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR6449674.1"
                     /translation="MNETGLILFSTDHCALCDEALGLLLSMPELAGISLRVVDVADDD
                     DLIATYGQRLPVLSYAGRELDWPFAATDLLRWIRQVEQP"
     gene            complement(2490..3308)
                     /locus_tag="AC16QF_13760"
     CDS             complement(2490..3308)
                     /locus_tag="AC16QF_13760"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR6449675.1"
                     /translation="MSERLTDIGVNLLNRQFQEDRQAVIDRAHRAGVTRMLITCTDLA
                     ESRLGQAFCQSNPEGFWCTAGVHPHHAKDVGPGWLEELAELAGQPCVRAVGETGLDFN
                     RNFSTPEQQLAVFDAQLKLAANTGKPVFVHDRDSDGKTFELLSRYREKLVGVVVHCFT
                     GTQTDLRHYLEAGFYIGITGWVCDPRRGGGLRDIVTMIPLDRLLIETDAPYLRPHNAP
                     VSPEGPARHKRRNEPALLGCVAAQLAELYGIGEADIARASWQNASRLFDLPDPA"
     gene            complement(3305..5200)
                     /locus_tag="AC16QF_13765"
     CDS             complement(3305..5200)
                     /locus_tag="AC16QF_13765"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR6449676.1"
                     /translation="MFFSLLFVAPVAPSFANDDEYRTPYQQRTAYRAALSHLRVGNTQ
                     AFRRIESDLKGYSLHPYLEYHELRARLSSASASEVLAFRERHADLPVTPLLYQRWLER
                     LGQGREWKAFLDHWEESGDPELRCYHSRALYGTGQKEAAFAGVADLWIVGRSQPEACD
                     PLFKVWIDNDNLTEGMVWQRLDLALQANKRQLARYLQRFFSGSRKPWAQSFYNVHVTP
                     DLIARTDRFETDNELSRAVIAHGLRRLAARDAEKASAAWQSYQRSHDFSPETVRELDQ
                     SIAIALAGEGSFPSQRPEKPSSEFVMEMAKAAVAQQNWSEAYYWIERLPSETLGSTRW
                     QYWSARAVGSTHLDSERARLTLQALAERRDYYGFLAAEHLGIETKLNHSALALNPIHT
                     LQLNRMPAVQRALELYAVGDLLNARREWNRVLPDLDQARQVQAAFLIQQSGWTSQAIR
                     MANAASLSDHLDLRFPLAYPEFFKRASHVTAIPESFLIAIARQESAFDPRARSHADAL
                     GLMQLLPSTAKRVARRTGLTAPSDDDLLNPAVNVEIASHHLAELMLRYGNRRPLAAAA
                     YNAGASRVDRWIRDAGGVPMDVWIETIPFGETRNYVKNVLAFAQVYGRLLGTPVPMLQ
                     VNETSIP"
     gene            complement(5283..5747)
                     /locus_tag="AC16QF_13770"
     CDS             complement(5283..5747)
                     /locus_tag="AC16QF_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6449677.1"
                     /translation="MADYRRIMLAVDLTEESNHVAERAIAIAKAFDAELHLVHVIEPL
                     SLAYGGDIPMDLSSVQEQIHDQAKSHLAEFANRLGVPAERQNLIFGRPESEIHRVAES
                     SEADLIVVGSHGRHGLALLLGSTANGVLHGATCDVLAVRVGSIAEKSPDSDS"
     gene            5821..6393
                     /locus_tag="AC16QF_13775"
     CDS             5821..6393
                     /locus_tag="AC16QF_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449678.1"
                     /translation="MTWLIIGLILLAAFGPVLWLVPSRKDRHLSALREQARLEGLVVE
                     LRRLPKLQPSAHERVTAGGRVKKPVQECAAYMQMLRRRLVMLPGWRVHRGTGDLEARG
                     GWYFDFELRARGDAFDATMSALDPLFGGLPADVVAVELTDRTLLAYWLERGAATRETV
                     SEMALQMRAAEQALLDLDARFQSLAENDDS"
     gene            6458..>6657
                     /locus_tag="AC16QF_13780"
     CDS             6458..>6657
                     /locus_tag="AC16QF_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR6449679.1"
                     /translation="MPPSLVIVESPAKAKTINRYLGSDYIVRSSVGHIRDLPVGGKSV
                     DPKARAREAAKTRKLSPEKKAE"
BASE COUNT         1202 a         2030 c         2249 g         1176 t
ORIGIN      
        1 tgccgtccca gggcatgttg gggtgatccc agcacatgaa gacgagatgt cccatgtctg
       61 gagaaaccgt cggcgatgag tagaagtcgt ggccggcgtg cagcgtggtg agctcgccgg
      121 ggtttcgcag ctcaattgcc tggagtctgt tcacgggctc gccctcggcc ccgtgctgct
      181 cggtcacggc aaccaggcgc tctctggccc cgtcccagca gaagtcggca aagcgcaggg
      241 attggtcgtt tcgagtgagc gcgctgatcg ctccgttggc gatgaggtaa atgcgttgat
      301 ccgaccagtt ggcaaagaac acgccctccg cgcagggcag gtaggcgccg ccaccgtact
      361 cgtgcacgcg cgtgcgaaca ttgaacggct ccggggtgag ctcggtgggc cgttctcccg
      421 gccgaagggc catgagcgtg gtgcgcccct gctctgccgg ccggctctcg agccagtaca
      481 gggtatcccc gtgagagcgc agatccctca gagcgatggc tccggaagcg atctcggctg
      541 cctgcaaagg ggagtgccag ttgccgtagg cgggagacgc catgggctca aggaagcagc
      601 gtggcgcgcg gatgaaaggc ggtccagcgc tcgtggtcgc gccatgaccc ggcaatgaac
      661 agaaacgacc tggacaggcc ctcgagctgg aaaccgcagc tgcggaccag ctcgatggag
      721 cggccgttct cgggctggat gttcgcttcc agacgatgca ggcccagttg gcagaaggcg
      781 taggttttga cgagttccag gccctcgcgc atgtagccgt gacccatttg cgtcgcgctg
      841 acgtagtagc ccagggaagc gctcagaaac gaacctcgga cgatgttgtt gatgttgatc
      901 acccccacga tgtcatcgtt gtccgtcagg cagaccaaca gcccctcgtg atcgtcctgc
      961 tcggtgcgct tgaggtagct gtgaaacgtt gcgtcgctca ggggtggaga tatccacggc
     1021 tcgtgaacgt gctggctgcg ctgcgtcata tgcagaaact cgctgcggtc ccggcgcgag
     1081 accggtcgga tgtagatgcg ttcagccacg gcttcttcgt cggctctcca gcacgaagat
     1141 ctcccacagc gcgggcggga tcatggcgag accaacgatg agcaggtgca gcggatgcaa
     1201 tcggtagtcg ttgtcgaaaa accagatgcc atgaaagccg aagccaacgg cgtgccagag
     1261 catgagtcca gccgtcgtca gtgcgaagcc catgcacagc agcgcgaagc gcgaaagata
     1321 tctcatggca gcaaaatata cgctgctccc cggtgcctaa gccaggctct ggagaaatcg
     1381 acggcagggt agcggcgcgg tacggaagcc gcttccggcg cccgcggatc gttgacgtga
     1441 acgtcctgcg gatcggcgcc gtgcacgacc acgagatgac cggcggtgcg ggtcagaggc
     1501 gctccgggca gctcgccctc gccgaagcgg acgctcgctg cgaatggcat tccagcatcc
     1561 agcacgctca gcggctcctc ccagtcgtcg aacaactcca ccgcaccaag ggatccttgc
     1621 tccgcagcgg cgcggatggc caggggccag attccgtaaa ggttggtcac cgcgtcgaag
     1681 caggattcgc tcacggactt cagatctgca gcgtgtccgt gccgcgcgag gagcatggtg
     1741 atgcaggcgg gcgagcacac gcgtggcccg acagacgtcc ccagtgccat ctgagagaga
     1801 aagggaggtg ccttgcagat cgccgatcgc gttggagcag gcgcacgttc gagctcgagc
     1861 tggcgcgccg tgacggtcaa gagatagcga gccgggctcg aagggccggt gacggtgacc
     1921 gtcagctcgg cgcgcggcag gtcctcgtga acgtgaaaac agtccacgtg cgtcgtgacg
     1981 gcggtgtcgg ccgacggctg cggcgccgcg cggcagggaa cctcgtgcag cgcccacagc
     2041 cgggaagcag cgcgaagcac gaactggaac cgctccgggg acggcgccgt cgagaggctg
     2101 ggtacgagaa ttgcgccggc cggtatgggc ggcagctgga tgatcgcggt atgtttgtca
     2161 ccggccgacc gccatccgat gggccccccg cagcgaagag ggtaccgctt gccggtgtca
     2221 gggtcggcga agcgttctgt cagtatcatg agttccagcg attgaccaac atgaacgaaa
     2281 ccggcctgat tctcttctcc acagaccact gcgcgctatg cgacgaggcg ctcgggctgc
     2341 tgttaagtat gccagaactg gccgggatct ccctgcgcgt cgtcgatgtg gccgatgacg
     2401 acgacctcat cgccacctat gggcagcggc tgccggtgct gagctacgcc gggcgcgagc
     2461 tggactggcc attcgccgcg accgacctgt tacgctggat ccggcaggtc gaacagccgt
     2521 gatgcgttct gccagctggc ccgggcgatg tccgcttcgc caatcccgta gagctcggcg
     2581 agctgcgcgg ccacacagcc gagcagagcg ggctcgttgc gcctcttgtg gcgcgccggc
     2641 ccttcaggag aaacgggcgc gttgtgcgga cgcagataag gcgcgtcggt ttcgatcagc
     2701 aggcggtcga gggggatcat ggtgacgatg tcgcgaagac cgcccccccg ccgcgggtcg
     2761 caaacccatc cggtgatgcc gatgtagaag ccggcctcca ggtagtgcct gagatcggtt
     2821 tgggtgccgg tgaagcaatg gacgaccacc cccaccagct tctcccggta tcggctgaga
     2881 agctcgaacg ttttgccgtc ggagtctcga tcgtggacga acacgggctt ccccgtgttt
     2941 gccgccagct tgagttgcgc gtcgaatacg gccagctgct gctccggcgt ggagaagttg
     3001 cggttgaagt ccaggccggt ttctcctacc gcccgcacgc agggttgccc cgcaagctcg
     3061 gcgagctcct ccagccagcc cggtcccacg tccttggcgt gatgcggatg gacgccggcc
     3121 gtgcaccaga aaccctcggg gttcgactgg cagaatgcct ggccgagccg ggattcggcg
     3181 aggtcggtgc aggtgatcaa catccgggtc actcccgccc ggtgagccct gtcgatcacg
     3241 gcctggcggt cctcctggaa ctgacggttg agcagattga cgccgatgtc ggtcaggcgc
     3301 tcgctcatgg gatgctggtc tcgttgacct ggagcatggg aacgggcgtg cccagaaggc
     3361 gcccgtagac ctgagcgaag gccaggacgt tcttgacgta gttgcgggtt tcaccaaagg
     3421 ggatggtctc gatccagacg tccatgggaa caccgccggc gtctctgatc cagcggtcga
     3481 cgcgactcgc gccggcattg taggcggccg cggcgagtgg tcgccggttg ccgtatcgga
     3541 gcatcaactc ggccagatga tggctcgcga tctccacgtt caccgcaggg ttgagcaggt
     3601 cgtcgtccga cggggcggtc agcccggttc gccgcgccac cctctttgcc gtgctgggca
     3661 gcagctgcat gagccccaac gcgtcggcgt gagatcgggc ccgcggatcg aaggcgcttt
     3721 cctggcgagc gattgcgatc agaaaggatt cgggaatggc ggtgacgtgg cttgcgcgct
     3781 tgaaaaactc gggataggcc agcgggaagc gcagatccag atggtccgac aggcttgccg
     3841 cgttggccat gcggatggcc tgtgaggtcc agccggattg ctgaatgaga aaggctgcct
     3901 gcacctggcg tgcctgatcc agatcgggca gcacgcggtt ccactcgcgc cgggcgttga
     3961 ggagatcgcc gacggcatac agctccaggg ctctttgcac cgctggcatg cgattcagct
     4021 gcagggtatg gatggggttc agtgccagcg ccgagtggtt cagcttcgtc tcgataccga
     4081 ggtgctccgc ggcgagaaat ccgtagtaat cgcgtctttc tgccaaggcc tgcagggtca
     4141 gcctggcgcg ctccgaatca aggtgagtgg acccgacggc ccgtgcggac cagtattgcc
     4201 agcgggtgct tcccagggtt tccgacggca gacgttcgat ccagtagtag gcctcggacc
     4261 agttctgctg ggccaccgcc gccttggcca tttccatcac gaattctgac gatggcttct
     4321 ctggccgttg cgatggaaaa gagccttcgc cggcaagcgc tatcgcaatc gattggtcca
     4381 gctcccggac ggtctccggg gaaaagtcgt gggaccgttg gtagctctgc cacgctgcgc
     4441 tggccttctc cgcgtctcgg gcggccaggc gccgcaatcc gtgggctatc acggctctgg
     4501 aaagctcgtt gtccgtctcg aagcggtcag tccgcgcgat gaggtctggc gtgacgtgca
     4561 cgttgtagaa cgattgagcc cacggctttc gtgaaccgga gaagaagcgc tgaagatacc
     4621 gggcgagctg acgtttgttg gcctgtagcg ccagatccag gcgctgccag accatgccct
     4681 ccgtcaagtt gtcgttgtcg atccacacct tgaacaacgg atcgcacgct tccggctggg
     4741 acctgcctac gatccacagg tccgcaacgc ctgcgaaagc agcctccttc tggccggtgc
     4801 cgtaaagggc ccggctgtga tagcaacgca gctccggatc gccgctttcc tcccagtggt
     4861 cgagaaacgc cttccactct cgcccctggc cgagtcgctc cagccagcgc tggtagagaa
     4921 gcggggtgac cggaaggtcg gcgtgccgct cgcgaaatgc cagcacctcg ctcgccgaag
     4981 cgctggacag gcgagcgcgg agctcgtggt actccagata cgggtgtagc gagtaaccct
     5041 tcaggtccga ctcgatgcgg cgaaacgcct gcgtattgcc gacccgcagg tgcgaaagag
     5101 cggcccgata cgcggtgcgc tgctggtatg gggtgcggta ctcgtcatcg ttggcgaacg
     5161 acggcgccac cggcgccacg aacaacagcg agaagaacat caggtgccag cgccgacagt
     5221 ggccgggcag agcggtgctg ggtgacggga gggtcagccg gccccttaga ctggcaatca
     5281 gatcagctat cggaatccgg cgacttctcc gcgatgctgc ccacgcgaac ggccagaacg
     5341 tcgcaggtgg cgccgtgcag cacgccgttg gcggtggagc cgagcagcag ggcaaggccg
     5401 tgccggccgt ggcttcccac cacgatgagg tcggcttcgc tggattcggc gacgcgatgg
     5461 atctcactct cggggcgtcc gaagatgagg ttctggcgct ctgccggaac gcccagacgg
     5521 ttggcgaact cggcgagatg ggatttagcc tgatcgtgga tctgctcctg aacagacgac
     5581 aggtccatgg gaatgtcgcc accgtaggca aggctcaatg gttcgatgac atgcacgaga
     5641 tgaagttccg cgtcgaaggc cttcgcaata gcgatagccc gctccgcgac gtgattggac
     5701 tcctcggtga gatccactgc caacatgatt cgacggtagt cagccatgtt gcctcccggc
     5761 acgaattgcg gcgacgatac acacaatcca aaggcccctc aacctctcgg ggagcgtccc
     5821 atgacgtggt taatcatcgg tttgatcctg ctagccgcct tcggtccggt gctctggctg
     5881 gtgcccagca gaaaagaccg ccacctcagc gcgttgcgag agcaggcccg actcgagggg
     5941 ctcgtggtgg aattgaggcg attgcccaag ctgcagccgt ccgcccatga gcgggtcacg
     6001 gccgggggac gcgtcaagaa accggtgcag gagtgcgcgg cgtacatgca gatgctgcgg
     6061 cgaagactcg tgatgctgcc cggctggcgc gtccatcgtg gcaccgggga tttggaagcc
     6121 cgcggcggct ggtatttcga cttcgagctc cgggcacggg gggatgcatt cgacgcgacg
     6181 atgtccgctc tggaccctct tttcgggggg ctccccgctg acgtcgtcgc ggtggagttg
     6241 accgaccgaa cgctgctcgc ctactggttg gaacgaggcg ctgccacccg ggaaaccgtg
     6301 tccgagatgg ccttgcagat gcgagcagcg gagcaagcgc tgctcgatct tgatgcccgt
     6361 tttcagagcc tggcggagaa cgacgattct tgacagccgt tttggccagg ccgtataacg
     6421 gcgcccgaac ccatcctcgg gcgctgttca ggcacatatg cccccttctt tagtcatcgt
     6481 tgaatcaccg gccaaggcca agacgatcaa tcgttacctt ggcagcgact acatcgtgcg
     6541 ctccagcgtg ggtcacattc gggatctgcc ggtgggcggc aagagcgtcg atcccaaggc
     6601 gcgagccagg gaggctgcca agacgcgcaa gctgtctccg gagaaaaaag cggagta
//
LOCUS       DBNHLM010000297        11812 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1659490, whole genome shotgun sequence.
ACCESSION   DBNHLM010000297 DBNHLM010000000
VERSION     DBNHLM010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11812
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1659490"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /gene="rlmB"
                     /locus_tag="AC16QF_13785"
     CDS             <1..594
                     /gene="rlmB"
                     /locus_tag="AC16QF_13785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR6449680.1"
                     /translation="VERVDRRQLDRLAGSGHQGVAAHCHELALADERAFAEHLDTLPE
                     PWLMLALDGVTDPRNLGACLRSAEAAGVQAVLLPRRRSAPLNAAALKAAAGAAEGLYL
                     VEVANLARRLEWLRDRGVWVVGAAGEADTPWTEADLDGNCLIVVGSEGQGLRQLTARS
                     CDQLVGIPMAGTVSSLNVSVAAGVLLFEAVRQRRARG"
     gene            716..1204
                     /gene="rpsF"
                     /locus_tag="AC16QF_13790"
     CDS             716..1204
                     /gene="rpsF"
                     /locus_tag="AC16QF_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR6449681.1"
                     /translation="MRHYEVVFLVHPDQSDQVPGMVERYQSLIERGTGVVHRLEDWGR
                     RQLAFPISKIHKAHYLLMNIEVNRETLEELESAFRFNDAVLRYLTIRRKAAVTGNSKI
                     YEEELRDQEKERERDQRREEETAQRRREREEAASAASDEAPAEEPAGESAATEAGEEV
                     TE"
     gene            1201..1425
                     /gene="rpsR"
                     /locus_tag="AC16QF_13795"
     CDS             1201..1425
                     /gene="rpsR"
                     /locus_tag="AC16QF_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR6449682.1"
                     /translation="MSRRFGRKKFSRLTGDDVNIDYKDLDLLKQYIGETGKIVPSRIT
                     GTSARHQRQLAQEIKRARFLALLPYTDQHK"
     gene            1436..1882
                     /gene="rplI"
                     /locus_tag="AC16QF_13800"
     CDS             1436..1882
                     /gene="rplI"
                     /locus_tag="AC16QF_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR6449683.1"
                     /translation="MNIILLEKIGSLGDLGEEVTVKGGFARNYLIPQGKAVRATTENR
                     GVFEERRAELERVAEEQRAAAQSRAEGLEGKVVTIVAKAGEEGKLYGSVGTQDIVDAL
                     HNEQIEIARSEVRMPEGVIRVTGEYEIDVQLHSDVTVTIQVNVVAE"
     gene            1968..3401
                     /gene="dnaB"
                     /locus_tag="AC16QF_13805"
     CDS             1968..3401
                     /gene="dnaB"
                     /locus_tag="AC16QF_13805"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR6449684.1"
                     /translation="MPDSATTPAEAHENETQRPAVAAVSHTAFKTPPHSVEAEQSVLG
                     ALMLNDEAWFNVAEIVTARDFYRPQHQIIFEAMAELSADTQPLDAVTVSERLQSKGLL
                     DKAGGNAYLGELAESIPGTSNVVAYAQIVREHSTLRQLISAANRIAETAFAPDGRPSG
                     ELLNMAEQEVFRIAEGRLRDSGPEKVVPLLHRAVERIEKLYETRNPITGVATGFDDLD
                     KKTAGLQPSDLVIIAARPSMGKTALAVNIVEHAVMEGSGAVLMFSLEMPSEQIVMRML
                     SSLGRIDQTRMRTGDMHEDDWPRFTSAVSQLKDKPLYLDDTPALTPNDIRTRARRVAR
                     ESGGLGLVVVDYLQLMRGSGPVENRTNEISEISRSLKAIAKELRCPVVALSQLNRGLE
                     SRTDKRPVMADLRESGAIEQDADVILFIYRDEVYNPESEQKGTAEIIIGKQRNGPIGM
                     CRLSYIGNLTKFENLAPDRYNDFQPFD"
     gene            3401..4771
                     /gene="radA"
                     /locus_tag="AC16QF_13810"
     CDS             3401..4771
                     /gene="radA"
                     /locus_tag="AC16QF_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR6449685.1"
                     /translation="MAGKRKAKSAYVCSDCGAEHTQWQGQCVACSAWNTLTRVSIGPI
                     KEPVSRGYAGSQSQVSLLGEVEREEAERLPTGFAELDRVLGGGLVPGSVVLMGGDPGA
                     GKSTLLLQVASRLAGRSGVLYVTGEESLHQLALRAERLQLAADQIRVAAETRAESVAE
                     LIAEYGPRVVIVDSIQVMQLESVDSTPGSVSQVRETAAFLTRLAKQTQTAVILVGHVT
                     KEGNLAGPKVLEHMIDCFMMLDSPAGSRYRTLRGLKNRFGAVNELGVFAMTEQGMKEV
                     ANPSAIFLQRGELDSPGSVATVTWEGTRPLLVEIQALVDEVQTGYAKRVAVGLDQQRL
                     AMHLAVLHRHCGITLGDQDVFANVVGGVRIGETGADLAALLAVLSSYRNRVLPKNLIV
                     FGELGLTGEVRAVANGQQRLSEARKHGFSQAIVPFANRPKEAISGMAVHGVKSLRAAM
                     DVIDTI"
     gene            complement(4772..6454)
                     /gene="ettA"
                     /locus_tag="AC16QF_13815"
     CDS             complement(4772..6454)
                     /gene="ettA"
                     /locus_tag="AC16QF_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HIR6449686.1"
                     /translation="MAQYVYTMQGVSKTVPPKRTILENISLSFFPGAKIGVLGLNGAG
                     KSSLLRIMAGVDTEFEGEARPQKDIRVGFLPQEPKLDDSLDVRGNVEQGMGEIMSDLA
                     EYNEISDKFAEPMSDDEMAELLDRQGELATRIDAANGWDVDRTLEIAADALRLPPWDA
                     KVAHLSGGERRRVALCTLLLSKPDMLLLDEPTNHLDAESVAWLERFLAEYPGTVVAVT
                     HDRYFLDNVAGWILELDRGRGIPFEGNYSAWLEAKEKRLEMEASKEKARQRAIHSELE
                     WVRSNPKARQAKSKARLQRFEELMSKETQSRNETAELYIPPGERLGEKVIELSHVSKG
                     FDDRLLIDDLSVSIPQGAIVGIIGGNGVGKSTFFRMLTGQEAPDGGSIELGDTVELAY
                     VDQSRDALADQKTVWEEIADSQDVIRVGSYEIQSRSYVSRFNFKGPDQQKRVGDLSGG
                     ERNRVHLAKLLRRGANVLLLDEPTNDLDVETLRALEEALLNFPGTVLVISHDRWFLDR
                     VATHILAFEGDSHVEWFAGNFSEYETDHRRRLAEQGLDTEPRRVRYKPLSRG"
     gene            6601..7866
                     /gene="glyA"
                     /locus_tag="AC16QF_13820"
     CDS             6601..7866
                     /gene="glyA"
                     /locus_tag="AC16QF_13820"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003340745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0006545 - glycine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR6449687.1"
                     /translation="MFGKDQQIAGFDDEVAAAINEETRRQEEHIELIASENYASPRVM
                     EAQGGVLTNKYAEGYPRKRYYGGCEWVDKVEELAIERVKALFGAHYANVQPHSGSQAN
                     AAAYLALLEPGDTMLGMSLDAGGHLTHGAAPNFSGKVYNPVQYGLDNATGEVDYEEVA
                     RLAEEHRPRMIIGGFSAYSRRMDWQRFRDIADAVGAYLLVDMAHVAGLVAAGLYPNPV
                     PIADVTTSTTHKTLRGPRGGIILARENAEIEKKLNSALFPGTQGGPLMHVIAAKAVCF
                     KEAQGEEFVEYQRQVIANARIMAAGFQSRGYKVVSGGTDNHLFLLDLIDKEITGKAAD
                     AALGRANITVNKNAVPNDPRSPFVTSGLRIGTPAVTTRGFREAECETLTGWMCDVLDD
                     IDNDNMIGSVRQKVLDLCRRFPVYEASAA"
     gene            7901..8359
                     /gene="nrdR"
                     /locus_tag="AC16QF_13825"
     CDS             7901..8359
                     /gene="nrdR"
                     /locus_tag="AC16QF_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR6449688.1"
                     /translation="MHCPFCGADDTKVIDSRLVAEGDSVRRRRECQTCGERFTTFETA
                     ELVMPRIVKSDGTREPFNEDKLRYGLQKALEKRPVSIEEIESALNRIMHRLRASGERE
                     LPSRDVGEAVMTELRTLDQVAYVRFASVYRDFQDLSEFQAEIQKLTFDLE"
     gene            8407..9501
                     /gene="ribD"
                     /locus_tag="AC16QF_13830"
     CDS             8407..9501
                     /gene="ribD"
                     /locus_tag="AC16QF_13830"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR6449689.1"
                     /translation="MAADSDLVYLQSAVELAARGMYTCTPNPRVGCIIVRDGTVLGRG
                     WHVRAGEGHAEVNAIADAGGFVEGATVYVSLEPCAFHGRTPPCSDTLIEARVGRVVAA
                     MTDPHPEVAGRGFAALQQAGIPVEVIEMPQALELNAGYRCRIAKGRPLVRLKTAISLD
                     GRTGMASGESKWITSEAARADVQAWRAQSCAIVSGSGTIMADDASLTVRDERFRVDGT
                     IRQPLRAIADSRLELPADAAVFAGPGQTILFHGPDAEPHVPDIEHVCLGVATGADRGK
                     VDLGGMLAELGRRGCNEVLVEAGARLLGAFIRAGLWDEMIVYVAPKLLGSDARPLAHL
                     PIETMSGAVQAKMIDCEPVGDDLRIRLARA"
     gene            9520..9954
                     /gene="nusB"
                     /locus_tag="AC16QF_13835"
     CDS             9520..9954
                     /gene="nusB"
                     /locus_tag="AC16QF_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR6449690.1"
                     /translation="MNPKPNPWARRRARRALVQAVYQWQITGADAATVTAEFGKALTR
                     ADGDFFDELLRGVLSSAAELDALFAPLLDRGVNELDQVERAILRLGTYELAHRIDVPY
                     RVVIDEYVELAKLFGAEEGYKYVNGVLDGLSHLLREPERRGG"
     gene            9951..10856
                     /gene="thiL"
                     /locus_tag="AC16QF_13840"
     CDS             9951..10856
                     /gene="thiL"
                     /locus_tag="AC16QF_13840"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HIR6449691.1"
                     /translation="MNEFDLIREILDVLGAATGGGAVRVGPGDDAAVIAAPEGADLVS
                     SIDAVVGDVHFPASAGASLIGYRSLMVSLSDLAAMGAEPGFALVALTLPEMDAEWTRQ
                     LASGMARAAQEASAPVVGGNIARGPLTITVSVHGWLPAGSAITRGGARPGHGVYVTGE
                     LGGAAAALTRGGLGERRVEADLDALDARYFLPAPRLEAGKRLRGLASSAIDVSDGLLQ
                     DLGHLCRASGTGAELDGASIPVMAGAKLEHALFGGDDYELCFTAAAEPGELGVACTRI
                     GTVVAGSEITLDARPVSAAGYQHFR"
     gene            10856..11344
                     /locus_tag="AC16QF_13845"
     CDS             10856..11344
                     /locus_tag="AC16QF_13845"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF016489.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A family protein"
                     /protein_id="HIR6449692.1"
                     /translation="MSTISWRDARDPAVLISCGFGSGFLPGAPGTWGSLAATALWWPL
                     LGGALWWVQAAGALLVLIAGTLLIGRVHRRHDVGDAPAIVIDEFAGMWLVLIGTGQDP
                     LLVAAAFGLFRLFDIWKPWPVRAVEARVRGGLGVMLDDVIAGAMALFVLQVSIWAIGG
                     GF"
     gene            11396..>11812
                     /locus_tag="AC16QF_13850"
     CDS             11396..>11812
                     /locus_tag="AC16QF_13850"
                     /inference="COORDINATES: protein motif:HMM:NF025341.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HIR6449693.1"
                     /translation="MKYLVRVLCVFSLVMPINAIASALPVEVVGLFKNHAVVRMQGGE
                     FVMKAGETRGGVTLVSADARQARMAYQGREHLVGLSERVSSQYRAVDKAQVMISSDSL
                     GQYWIRGAINNRFTNFLVDTGASVVAISAVHARQLGI"
BASE COUNT         1999 a         3844 c         3983 g         1986 t
ORIGIN      
        1 gtcgagcggg tcgaccgacg tcagctcgat cgcctggcag gctcgggcca ccagggcgtc
       61 gccgcgcact gccacgagct ggccctggcc gacgagcgtg cgtttgccga gcatctcgac
      121 acccttcccg agccttggct catgctggcg ctcgacgggg tcacggatcc gcgcaatctc
      181 ggcgcctgcc tgcgctcggc tgaggcggcg ggcgtgcagg cggttctgct tcccaggcgg
      241 cgcagcgcgc ccctgaacgc ggcggctctg aaagcggcgg ctggcgcagc ggaaggcctc
      301 tatctggtgg aggtcgccaa tctggcccgc cggctcgagt ggcttcggga ccgcggcgtc
      361 tgggtggtgg gcgcggccgg cgaggccgat acgccctgga cggaggcgga tctcgacggc
      421 aactgtctca tcgtcgtcgg cagtgagggg cagggtctgc gacagctcac cgcgcgcagc
      481 tgtgatcagc tggtgggcat ccccatggcg ggcaccgtgt cgagcctgaa cgtctccgtg
      541 gccgccggcg tgctcctttt cgaggccgtt cggcagcgcc gcgccagggg ctgagcgccc
      601 ttgcatccga tctggcgcct acctacaatt cgcgtcccct cgtcggaggg gtccttgcct
      661 cacgccgcaa acctctgcga gcggaggcgg taacgccaaa aggagaattc gcaacatgcg
      721 acattacgaa gtcgtttttc tcgtgcaccc cgatcagagc gatcaggtgc ccggaatggt
      781 cgaacgctac cagagcctca tcgagcgtgg caccggcgtc gtccatcgtc tggaggactg
      841 gggccgccgc cagctggcct ttcccatctc gaagattcac aaggcccatt acctgctgat
      901 gaacatcgag gtcaaccgtg agaccctgga ggagctcgag agcgcgttcc gtttcaacga
      961 cgcggttctg cggtatctga ccatccgccg caaggcggcg gtcaccggca actccaagat
     1021 ctacgaggag gagctgcgcg accaggagaa agagcgcgag cgcgaccagc gtcgtgagga
     1081 agaaacggct cagcggcgcc gtgaaaggga ggaggccgct tccgcggctt ccgatgaagc
     1141 gcccgcggag gagcccgccg gcgaatcagc cgcaactgaa gccggagagg aggtgacgga
     1201 atgagcagac gattcggacg caagaagttc agccggctga ccggcgacga tgtcaacatc
     1261 gactacaagg atctggatct gctcaagcag tatatcggcg aaacggggaa gatcgtcccc
     1321 agccggatca cgggcaccag cgcgcgccat cagcgtcagc tggcccagga gatcaagcgc
     1381 gccaggttct tagcgcttct gccatacacc gatcagcaca agtaggtctt cgatcatgaa
     1441 catcattctg ctggaaaaga tcggcagcct cggcgatctc ggcgaggaag tgaccgtcaa
     1501 aggtgggttt gccaggaact atctaatccc tcagggaaag gccgttcggg cgaccaccga
     1561 gaatcgggga gtgttcgagg agcgcagggc ggagctcgag cgtgtggccg aagagcagcg
     1621 tgcagctgcc cagagccgcg ccgagggact cgaaggcaag gtcgtcacca tcgtggcgaa
     1681 ggccggcgag gagggcaagc tgtacggatc cgtgggaacc caggacatcg tggatgccct
     1741 gcacaacgag cagatcgaga ttgctcgcag cgaggttcgc atgcccgaag gcgtgatccg
     1801 ggtaaccggc gagtacgaaa tcgacgtgca gctgcactcc gacgtgaccg tcacgattca
     1861 ggtgaacgtc gtcgccgagt aatttctcgc gccgataggc aatcttcgct tgcaatgggg
     1921 accggaaaga cagagtcttt tcctcccccg cggaaagtcg tccagcgatg cctgacagcg
     1981 caaccacgcc cgcagaggct cacgaaaacg aaactcagcg accagccgtc gcggccgtga
     2041 gccacacggc gttcaagacg ccgccccact ccgtagaggc cgagcagtcc gtgctgggcg
     2101 cgctgatgct gaacgacgaa gcgtggttca acgttgccga gatcgtcacc gcccgggact
     2161 tctaccggcc gcagcatcag atcatcttcg aagccatggc ggagctgtcg gcagacacgc
     2221 agcctctgga cgcggtgacc gtctcggagc ggctgcagtc caagggcctg ctcgacaagg
     2281 cgggcggcaa cgcttacctg ggcgagctgg ccgagtcgat tcccggcacg agcaacgtgg
     2341 tggcctacgc gcagatcgtg cgcgagcact ccacgttgcg tcagctgatc agcgcggcca
     2401 accgcattgc ggaaacggcg ttcgctcccg acggcaggcc cagcggggag ctgctgaaca
     2461 tggccgagca ggaggtcttc cgcatcgccg aagggcgtct gcgcgacagt ggacccgaaa
     2521 aggtcgtccc gctgctgcac cgggcggtgg agcgcatcga gaagctctac gagacccgca
     2581 accccattac cggcgtggcc accggcttcg acgatctgga caagaagacg gccggcctgc
     2641 agccgtccga tctggtgatc atcgccgcgc ggccgtccat gggcaaaacg gcccttgcgg
     2701 tcaacatcgt cgagcacgcg gtgatggaag gcagcggcgc cgtgctcatg ttcagcctgg
     2761 agatgccgtc cgagcagatc gtcatgcgca tgctctcatc gctgggcagg attgatcaga
     2821 cccgcatgcg caccggggac atgcacgagg acgactggcc gcggttcacc tccgccgtca
     2881 gccagctgaa agacaaacct ctgtacctgg acgatacgcc tgcgctgacg ccgaacgaca
     2941 tccgcacccg ggcgcggcgg gtagcccgcg aatccggcgg cctcggcctg gtggtggtcg
     3001 actacctgca gctcatgcgc ggttccggac ctgtggagaa ccgcacgaac gagatttcgg
     3061 agatctcccg atctctgaag gccatcgcca aggagctgcg ctgcccggtg gtcgcgcttt
     3121 cccagctcaa tcgggggctg gagagccgta ccgacaagcg gccggtcatg gccgatctta
     3181 gagagtcggg cgccatcgag caggacgcgg acgtgatcct tttcatctac cgcgacgagg
     3241 tttacaaccc cgaatccgag cagaagggca ccgccgagat catcatcggc aagcagcgca
     3301 acggccccat cgggatgtgc cgtctgtcct atatcggcaa cctgaccaag ttcgaaaacc
     3361 tggcgccgga tcgctacaac gactttcagc catttgactg atggcgggca agcgcaaagc
     3421 caaatccgcg tacgtctgct cggactgcgg cgccgagcat acccagtggc agggccagtg
     3481 cgtggcctgc agcgcctgga acaccctcac ccgggtttcc atcgggccca tcaaagaacc
     3541 cgtttcccgg ggctacgccg gcagccagag ccaggttagc ctgctcggcg aggtggagcg
     3601 cgaggaggcc gagcgtttgc ccaccggatt tgccgagctc gaccgggtgc tcggcggcgg
     3661 cctggtgccc ggctccgtcg tgctgatggg tggagatccc ggcgcgggaa agagcacgct
     3721 gctgctgcag gttgccagcc ggctggcagg gcgcagcggg gttctctacg tgaccggcga
     3781 agagtcgctc caccagctgg cgctgcgcgc cgagcgtctg cagctggcgg cggatcagat
     3841 tcgcgtggcg gcggagaccc gtgcggaatc ggtggccgag ctcatcgccg agtacggtcc
     3901 gcgggtcgtc atcgtggact ccattcaggt catgcagctg gagtccgtgg acagcacgcc
     3961 gggcagcgta tctcaggtcc gcgagaccgc cgccttcctc actcgcctcg ccaagcagac
     4021 ccagacggcg gtcattctcg ttggccacgt gaccaaggag ggtaatctgg cggggcccaa
     4081 ggtcctcgag cacatgattg attgcttcat gatgctcgac agccccgccg ggagccgtta
     4141 ccgcacgctg cgcggtttga agaaccggtt tggagcggtc aacgaactgg gcgtcttcgc
     4201 catgaccgag cagggcatga aggaggtcgc caacccgtca gccatcttcc tgcagcgcgg
     4261 cgagctcgac tcgcccggca gcgtcgccac ggtcacctgg gaaggcacgc gcccactgct
     4321 cgtggagatc caggctctcg tggacgaggt gcagaccggc tacgccaagc gggtcgccgt
     4381 gggcctggac cagcagcggc tggccatgca cctcgccgtg ctgcaccggc actgcggcat
     4441 cactcttggc gatcaggatg tctttgccaa cgtggtcggc ggcgtgcgca tcggggagac
     4501 gggcgcggat ctggcggcgt tgctggcggt gctgtcgtcc taccgcaatc gtgtgctgcc
     4561 caagaacctc atcgtgttcg gagagctcgg cctgaccggc gaggtgcgtg cggtggccaa
     4621 cgggcagcag cgtctgtccg aagcccgcaa gcacggcttc agccaggcga tcgtgccgtt
     4681 tgcgaaccgg cccaaggagg ccatttccgg catggccgtc cacggggtca agagcctgcg
     4741 tgccgccatg gacgtgatcg acacgatctg atcagccgcg ggaaagcggc ttgtagcgca
     4801 cgcggcgggg ctccgtatcc agcccctgct cggcgagccg cctgcggtgg tcggtttcgt
     4861 actccgagaa gttccccgca aaccactcca cgtgcgagtc tccttcgaaa gccaggatgt
     4921 gggtggccac acggtccagg aaccagcggt cgtgggatat caccaggacg gttcccggaa
     4981 agttcagcaa cgcttcctcc agcgcgcgca gggtctccac gtccaggtcg ttggttggct
     5041 cgtccagcag cagcacgttc gcgccgcggc gcagcagctt ggccagatgc acgcggttgc
     5101 gctcgccgcc cgagagatcg ccgacccgct tctgctggtc cggacctttg aagttgaacc
     5161 gggagacgta gctgcgcgac tggatctcat agcttcccac gcggatgacg tcctgactgt
     5221 cggctatctc ctcccatacc gtcttctggt cggccagggc gtcccggctc tggtccacgt
     5281 aggccagctc cacggtgtcg cccagctcga tgctcccgcc gtccggcgcc tcctgcccgg
     5341 tcagcatacg aaagaaggtc gacttgccca ccccgttgcc gccgatgatg ccgacgatgg
     5401 cgccctgagg gatggatacg ctcagatcgt cgatgagcag ccggtcgtcg aagcccttgc
     5461 tcacgtggga cagctcgatg accttttccc ccagccgctc ccctggcggg atgtagagct
     5521 cggcggtttc gttgcggctc tgggtctcct tggacatgag ctcctcgaag cgctgcaggc
     5581 gagccttgct cttggcctgc cgggccttgg ggttcgatct cacccactcg agctcagagt
     5641 ggatcgcccg ctggcgggct ttctccttgg acgcttccat ctccagccgt ttctccttgg
     5701 cttccagcca tgcgctgtag ttgccctcga acgggatgcc gcggccgcga tcgagctcca
     5761 ggatccagcc ggcgacgttg tctaggaaat agcggtcgtg ggtgacggcg accacggtcc
     5821 ccgggtactc ggcgaggaag cgctccagcc aggccacgct ttcggcgtcg aggtgattgg
     5881 tcggctcgtc cagcaggagc atgtccggct tggagagcag cagggtgcac agagccaccc
     5941 gccgccgctc accgccggag aggtgggcaa ctttggcatc ccacggcggc aggcgcaggg
     6001 cgtccgcggc gatctccagg gtccggtcca cgtcccagcc gttggccgcg tcgatcctgg
     6061 tggcgagctc accctgacga tccagcagct ccgccatctc gtcgtcggac atgggctcgg
     6121 cgaacttgtc ggagatctcg ttgtactcgg cgaggtccga catgatctcg cccatgccct
     6181 gctcgacgtt gccgcgcacg tccagggaat cgtcgagttt cggctcctgg ggaagaaacc
     6241 ccacccggat gtccttctgg gggcgagcct cgccctcgaa ctcggtgtcg acgccggcca
     6301 tgatcctgag caggctcgac ttgccggccc cgttgaggcc gagcacgccg atcttcgccc
     6361 cgggaaagaa cgaaagggag atgttctcga ggatcgtcct cttgggtggg accgtcttcg
     6421 agacgccctg catggtgtag acgtactggg ccaaggatgc cgctcctggt cgatggactc
     6481 agagagcgcg caagctaccg aaatcgccgc ggtggttcaa ttcaatttcc ccatgaaagg
     6541 attgcgtacg agatggcgcc gagctggggt aaacttgcgc gttttcgacc gggggcgccc
     6601 atgtttggca aagatcagca aattgccggg tttgacgacg aagttgccgc agccatcaac
     6661 gaagagacgc gacgccagga agagcacatc gagctcatcg cctcggagaa ctacgcaagc
     6721 ccccgggtca tggaggccca gggcggcgtg ctgaccaaca agtacgccga aggctatccc
     6781 cgcaagcgtt actatggcgg ctgcgagtgg gtagacaagg tggaagagct ggccatcgag
     6841 cgggtgaaag cgctgttcgg cgcgcactac gccaacgtcc agccccactc cggatctcag
     6901 gccaacgcgg ccgcctacct tgccctgctc gaacccggcg acaccatgct gggcatgagc
     6961 ctggacgccg gcggccacct cacgcacggc gctgccccca atttttccgg caaggtctac
     7021 aacccggtgc agtacggcct ggacaacgcc acgggagagg tggactacga agaggtagcc
     7081 cgccttgccg aggagcaccg gccccgcatg atcatcggcg ggttttccgc ctattcccgg
     7141 aggatggact ggcagaggtt tcgcgacatc gccgatgccg tcggggccta tctgctggtg
     7201 gacatggctc acgtcgccgg gctcgtggct gccggtctct acccgaaccc ggtaccgatc
     7261 gccgacgtga ccacctccac cacccacaag acgcttcgtg gaccgcgcgg cggcatcatc
     7321 ctggcccgcg agaacgcgga gatcgaaaag aagctcaact ccgcactctt cccgggaacc
     7381 cagggcggcc cgctcatgca cgtgatcgcg gccaaggccg tgtgcttcaa ggaagctcag
     7441 ggcgaggagt tcgtggagta ccagcgtcag gtcattgcca atgcgcggat catggccgcc
     7501 ggcttccaga gccgcggcta caaggtggtg tccggcggta cggacaacca tctctttctg
     7561 ctcgacctca tcgacaagga aatcaccggc aaggcggctg acgctgccct ggggcgcgcc
     7621 aacatcacgg tcaacaagaa cgcggtgccc aacgaccccc gctcgccgtt cgtcacctcg
     7681 ggtctgcgca tcggcacccc ggcggtcacg acccgcggct tccgcgaagc ggagtgcgag
     7741 accctgacgg ggtggatgtg cgacgtcctc gacgacatcg acaacgacaa catgatcggc
     7801 agcgtccgac agaaggtgct ggatctgtgc cgccggttcc ccgtttacga ggccagcgcg
     7861 gcctgagccg caaggccccg gcttcgcctg cgcagacgcc gtgcattgcc ccttctgtgg
     7921 tgccgacgac accaaggtga tcgactcccg gctggtggct gagggggact cggttcgccg
     7981 ccggcgcgaa tgccagacct gtggcgagcg attcacaacc ttcgagaccg ccgagctcgt
     8041 catgccgcgc atcgtcaaga gcgacggcac ccgcgagccg ttcaacgagg acaagctgcg
     8101 ttacggtctg cagaaagcgc tggagaaacg tccggtcagc atcgaggaga tcgagagcgc
     8161 cttgaaccgc atcatgcacc ggctgcgggc cagcggggag cgtgagcttc cgtcccggga
     8221 cgtgggcgag gctgtcatga ccgagctgcg caccctcgat caggtggcct acgtgcgttt
     8281 tgcttcggtt taccgtgatt ttcaggactt gagcgagttt caggcggaga tacagaagct
     8341 caccttcgac ctggaatagc ccgggcgaat agccacggcg gacgcgaacg cctatatttg
     8401 cgcgccatgg ccgccgactc ggatctcgtc tacctgcaaa gcgccgtgga gctggccgcg
     8461 cgaggcatgt acacgtgcac gccgaaccca cgtgtgggat gcatcatcgt ccgtgacggc
     8521 accgtgctcg gccgcggctg gcacgtgcga gccggtgaag gccacgcgga ggtgaacgcc
     8581 atcgccgacg ccgggggctt cgtggaaggt gccaccgtat acgtttccct cgagccctgc
     8641 gcttttcacg gccgcacgcc gccgtgcagc gacactctca tcgaggcgcg ggtcggtcgc
     8701 gtggtggcgg ccatgacgga tccgcatccc gaggtcgccg ggcggggctt cgccgcgctg
     8761 cagcaggctg gcataccggt tgaagtcatc gagatgccgc aggccctgga gctgaacgcg
     8821 ggctatcgct gccggatcgc caagggccgc cccctggtca ggctcaagac cgccatatcc
     8881 ctggacgggc gcacgggaat ggcgtcgggg gagagcaagt ggatcaccag cgaggcggcg
     8941 cgtgccgacg tgcaggcatg gcgcgcccaa agctgcgcca tcgtcagcgg ctcgggaacc
     9001 atcatggcag acgatgcgag cctcaccgtc cgcgacgagc ggttccgggt ggacgggacg
     9061 atccgtcagc cgctgcgggc catcgccgat tcccggctgg agctgcccgc ggacgccgcc
     9121 gtctttgccg ggcccggcca gaccatcctc tttcacggcc cggacgccga accccacgtg
     9181 cccgatatcg agcacgtctg cctcggggtt gcgacgggag ccgatcgggg caaggtggac
     9241 ctcggcggga tgctggcgga gctcggccgc cggggctgca acgaggttct ggtggaagct
     9301 ggcgcgaggc tgctgggcgc gttcatccgc gccggcttgt gggacgagat gatcgtctac
     9361 gtcgcgccca agctgctcgg cagcgatgcg cggccgctgg ctcaccttcc catcgagacc
     9421 atgagcgggg cggttcaggc taaaatgatc gattgcgagc cggtgggcga cgatctgagg
     9481 atccgcctgg cgcgcgcctg aacgggaccc ctatcagcaa tgaaccccaa gccgaatccg
     9541 tgggcccgcc gccgcgcccg aagggccctg gtccaagccg tgtaccagtg gcagatcacg
     9601 ggcgccgatg cggcgacggt cacggcggag ttcggcaaag cgctcactag ggccgacggc
     9661 gacttcttcg acgagctgct gcgcggcgtg ctgagctcgg cagccgagct ggatgcgctt
     9721 ttcgcgcccc tgctggaccg gggcgtgaac gagctcgatc aggtcgagcg cgcaatcctg
     9781 cgcctcggca cttacgagct cgcccatcgc atcgacgttc cctaccgcgt ggtcatcgac
     9841 gagtacgtcg agcttgccaa gctctttggc gccgaggagg gctacaagta cgtcaacggg
     9901 gttctggacg gactgtccca cctcctgagg gaaccggagc ggcggggcgg gtgaacgagt
     9961 tcgatctcat cagagagatc ctcgacgtgc tgggcgcggc caccggaggt ggcgccgtcc
    10021 gcgttggccc cggcgacgac gccgcggtga tcgcggcgcc tgaaggcgcc gatctcgtct
    10081 cctccatcga cgccgtggtc ggcgacgtcc actttcctgc ttcggctggc gcttccctca
    10141 tcggttaccg gtcgctgatg gtgagccttt cggacctggc cgccatgggt gcggagccgg
    10201 ggttcgccct ggtagcgctg acgcttccgg agatggacgc cgagtggacg cggcagcttg
    10261 cgtccggcat ggcccgggcg gcgcaagagg cgtccgcgcc cgtggttggc ggcaacatcg
    10321 cgcgtggacc gctgaccatc acggtgagcg tgcacggctg gttacccgcg ggttcggcca
    10381 ttacccgtgg cggcgcccgt cccgggcacg gcgtgtacgt caccggagag cttggtgggg
    10441 ctgcagctgc ccttacccgg ggtgggctcg gtgagcgccg ggtggaagcc gacctcgacg
    10501 ctctggacgc tcgctacttt ctgcccgcac cgcgcctcga ggcgggaaag cggctgcgcg
    10561 gtttggccag cagcgccatc gacgtgtccg acgggctgct gcaggacctc gggcacctgt
    10621 gccgtgccag cggcaccggg gccgagctcg acggcgcatc gatcccggtc atggccggcg
    10681 cgaagctcga gcacgcgctc tttggcggcg acgattacga gctgtgcttc accgccgccg
    10741 cggagcccgg ggagctcggt gtcgcctgca ccaggatcgg caccgtggtg gcggggtcgg
    10801 aaatcacgct ggacgctcgg ccggtttcgg ccgccggcta ccagcacttt cgctgatgag
    10861 cactatctcc tggcgcgacg cgcgcgaccc cgcagtattg atctcctgcg gcttcggcag
    10921 cggctttctg ccgggagcgc cgggtacttg gggcagcctg gcggcgacag cgctctggtg
    10981 gccgctcctt ggcggtgcgc tctggtgggt ccaggccgcc ggggccttgc tggtgctaat
    11041 cgcgggcacg ctgctcatcg gccgggtgca ccgacgccac gacgtcggtg atgcgcccgc
    11101 catcgtcatc gacgagtttg ccggcatgtg gttggtgctg atcggtaccg gccaggatcc
    11161 tctcttggtc gccgccgcct tcggcctgtt tcgcctgttc gacatctgga agccctggcc
    11221 ggttcgtgcc gtggaagccc gcgtgcgcgg aggcttgggc gtcatgctcg atgacgtcat
    11281 cgccggcgcc atggcgcttt ttgtgctgca ggtctcaatc tgggccattg gcggcgggtt
    11341 ttagcgtcga ggctgactac actcgaaggc cgtcaagtcg cgttcacgga cgaccatgaa
    11401 atacctcgtg cgggttctct gcgtgttctc tctcgtcatg ccgatcaacg cgatcgcttc
    11461 ggctttgccc gtggaggtgg tggggctgtt caagaaccac gccgtggttc gcatgcaggg
    11521 cggcgagttc gtgatgaaag cgggcgagac ccgcggcggg gtgaccctcg tctccgcgga
    11581 tgccaggcag gcgcgcatgg cctatcaggg ccgggagcac ctcgtcgggc tgtccgaacg
    11641 cgtttccagt caataccgcg cggtcgacaa ggcccaggtg atgatcagct ccgattcatt
    11701 gggccagtac tggattcgcg gcgccatcaa caatcgcttc accaacttcc tcgtcgacac
    11761 cggcgcgtcg gtggtcgcca tcagcgcggt gcatgcccgg cagctgggca tc
//
LOCUS       DBNHLM010000298         4541 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1061086, whole genome shotgun sequence.
ACCESSION   DBNHLM010000298 DBNHLM010000000
VERSION     DBNHLM010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1061086"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..959)
                     /locus_tag="AC16QF_13855"
     CDS             complement(186..959)
                     /locus_tag="AC16QF_13855"
                     /inference="COORDINATES: protein motif:HMM:NF024375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6449694.1"
                     /translation="MTSSDWGRVRPADIPWKQFDDPHGRPTTPVRVLKEGDPAVLEVR
                     FPPGYVAGLHWHPYDTLYIVTRGSMQIGDEGVYRPGDIRWVRAGHVYGPEAAGAEGVD
                     FFLVSLGGEIELNWADLVDVPERLRERLETSGEPWGRVNLQEVPWQDFEDPAGRPTQP
                     VQVLLDDDPYVLRTRFSPDYVAGDHWHDFDTLYFITSGSMRFGDEGWYEPGEVRWVRG
                     GHSYGPEEPGEGGVEFILVSCGGPVNLHWSDLEPPPSKP"
     gene            1045..2034
                     /locus_tag="AC16QF_13860"
     CDS             1045..2034
                     /locus_tag="AC16QF_13860"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6449695.1"
                     /translation="MSLASAQTIDVHAHAVLEETMGAAGEHGPELGREAAPRFRVGNY
                     ELHGVRYRGSPFMDPDLRLDAMDAAGIDYQVLSPNPLTYFHFIDAGAARDFCRIHNDT
                     LSAIVGERADRLGAFAALPMQDVDFAIEETERAVRELGMYGPYIGTDFGRPLNDPALD
                     PFYEKLIELDVPLFIHPAPAGIDGPPGDPNLKQFDLDIIIGFAAQETIATCTLIYGGV
                     LERHPKLDVCLSHGGGATGYAFGRMASAAKKRPWASDSLRQDGAFEALLQRLWFDVHV
                     HDPDALALLHKKVNPDHLVFGTNFAGWDQQPGDVRHDAAPYTENARRLLRATR"
     gene            2031..3140
                     /locus_tag="AC16QF_13865"
     CDS             2031..3140
                     /locus_tag="AC16QF_13865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6449696.1"
                     /translation="MTSSPVNPLRSDEHPFKLAVFGINVSGGCTMTRAPGTLEVSWPE
                     TRQVALAAEQAGFDALIPVARWKGMGGAVNFNHRSWEPFTLASALAAITERIALFSTC
                     HVPTVHPVRLAKESATVDQISGGRFCLNIVAGWNEREIGMFGVQQKPHDERYDVADDW
                     IHLTKRLWTETQEFDHQGPYFSAPGAYSEPKPVQAPHPLIMSAGNSPRGLAFAAEHAD
                     LNFVVAADLDKAGESASAVRAKAAEQGRSIKVFGQGYIVCRSSEAEARAARDRYVHEL
                     GDWEGVRNLLDVLIPNSQSALGDEWEAMAANMIAGYGALPLVGTPDQVVDGMQAFSDA
                     GLDGMTLSWLDYPAGIEQFEKELRPRLVEAGLRAR"
     gene            complement(3137..3976)
                     /locus_tag="AC16QF_13870"
     CDS             complement(3137..3976)
                     /locus_tag="AC16QF_13870"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6449697.1"
                     /translation="MLYGIKTSPQLTTWHDMLDVWRAADGMEVFHSAWNFDHFYPINV
                     PDTSGPCMEAWTTLTALAQATERIRIGCMVSGVVYRHPAVLANMIASLDIISDGRLEV
                     GLGAGWNEEECDAYGIELGTLTERFDRFDEACAVIHGMLSVPTTDFAGRYFTLTAARC
                     NPPPLQQPHPPICIGGNGERRTFPNVVRWAQHWNYPGFDVDGFVAKRDVLWDQCRAVG
                     RDPGEIMTSAHQPVDLDDLDDLRRRVTAFREAGLGLLIFYLPPPHRALALEPIAEIAA
                     QVG"
     gene            4150..>4541
                     /locus_tag="AC16QF_13875"
     CDS             4150..>4541
                     /locus_tag="AC16QF_13875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6449698.1"
                     /translation="MSFKRALVVDDSKSARLSLKKLLEQHHLEVDEAFSGEEALDFIK
                     DHSVDVIFMDHTMPGMDGLEAVSAIKSNPKTATIPVMMYTTQEGEVYVGQARALGAVE
                     VLPKQVQPGVLFEMLEKLGLVTDRRTED"
BASE COUNT          853 a         1507 c         1419 g          762 t
ORIGIN      
        1 tacacgacgg tgtcgcgaga aacctcgcga acgtccttca tgcggccgat ccagaatccg
       61 actccgggca cctgcaccac ccgcgcatcg gcagcatgaa gcgccctgta gaacggatag
      121 gggtcgcgca tcacttccgg gtcggtcagg gtgtggtcat tcagcagatc catctcttgt
      181 gcgtcctagg gtttcgaggg cggcggttcc agatccgacc agtgcagatt gacggggccg
      241 ccgcaactga cgaggatgaa ctccaccccg ccttctccag gctcttccgg tccgtagctg
      301 tgaccgcccc gcacccagcg gacttcacca ggctcgtacc agccctcatc gccgaaccgc
      361 atcgagccgg acgtgatgaa gtaaagcgtg tcgaaatcgt gccagtggtc gccggcaacg
      421 taatccggtg agaatcgggt tcgcaggacg tagggatcat cgtccaggag aacctgaacc
      481 ggctgggtgg gtcgtccggc gggatcctcg aagtcctgcc acggcacctc ttgaaggttt
      541 acccgccccc acggctcgcc cgaggtctcc agtcgttcgc gcaaccgctc cggcacgtcg
      601 accaggtcgg cccagttaag ctcgatctcg ccgccgaggc tcaccaggaa aaagtccacg
      661 ccttcggcgc cggcggcctc cggcccgtaa acgtgccccg cccgtaccca gcgaatgtct
      721 cccgggcgat acacaccttc atcacctatc tgcatcgatc ctcgagtgac gatgtagagg
      781 gtgtcgtatg ggtgccagtg cagcccggca acgtaacccg gcgggaaccg gacctcgaga
      841 acggcggggt cgccctcttt gagaacgcga accggggtgg tcggtcgtcc gtgaggatcg
      901 tcgaactgct tccagggtat gtcggcgggc cgcacgcgac cccaatcaga actcgtcatg
      961 acgttgatct taccatccga ccgggtccat catcgctctg gtttcggtga gtgaacccac
     1021 ttcagcagcc cgaggaaacg acacatgagc cttgcgagcg cccagaccat cgacgtccat
     1081 gcccacgccg ttctggaaga gaccatgggg gccgcgggcg agcacggccc ggagctcggt
     1141 cgcgaggctg ccccgcgctt ccgagtgggc aactacgagc tgcacggcgt gcgctaccgc
     1201 ggcagcccgt tcatggaccc ggatctgcgc ctcgacgcca tggacgcggc cggaatcgac
     1261 taccaggtcc tgtcacccaa cccgctcacc tactttcact tcatcgatgc cggcgccgcg
     1321 agggatttct gcaggatcca caacgacacc ctgtctgcca tcgtcggcga gcgggcagac
     1381 cgtctcggcg cgtttgccgc gctgcccatg caggacgtgg acttcgccat cgaggagacg
     1441 gagcgggcgg tgcgcgagct cggcatgtac ggtccctaca tcggcaccga cttcggacgg
     1501 ccgctgaacg accccgcgct cgatcctttc tacgaaaagc tcatcgagct ggacgttcct
     1561 cttttcatcc accccgcccc cgcgggcatc gatggcccgc cgggggatcc caacctcaag
     1621 cagttcgatc tggacatcat catcggcttc gcggcgcagg aaacgattgc cacctgcacg
     1681 ctcatctacg gcggggtgct ggagcgtcat ccgaagctgg acgtctgctt gagtcatggt
     1741 ggcggggcca cgggatacgc cttcggaagg atggcgagcg cggcgaaaaa gcgcccctgg
     1801 gcatccgact cgctgcgcca ggacggcgcg ttcgaggctc tgctccagcg tctgtggttc
     1861 gacgttcacg tgcacgatcc ggacgctctg gcccttctgc acaaaaaggt gaaccccgat
     1921 caccttgtgt tcggcaccaa ttttgcgggc tgggaccagc agccgggaga cgtccgtcac
     1981 gacgccgcgc cgtacaccga gaacgcgcgt cgcctgctcc gcgcaacgcg atgaccagct
     2041 cgcccgtcaa cccgctgcgc agcgacgagc accccttcaa gctcgccgtg ttcggcatca
     2101 acgtcagcgg tgggtgcacg atgacccgtg ctccaggcac cctggaagtg agctggcccg
     2161 agacccgcca ggtagcgctg gccgccgagc aagcggggtt cgatgcgctg atacccgtgg
     2221 cgcggtggaa gggcatgggc ggcgcggtga acttcaacca ccgcagctgg gagccgttca
     2281 cgctggcaag cgcgctggcg gcgatcaccg agcggatcgc gctcttctcc acctgtcacg
     2341 tgcccaccgt gcatcccgtg cgtctggcga aggaatcggc aactgtggat cagatctccg
     2401 ggggcaggtt ctgcctgaac atcgtagcgg gctggaacga gcgggagatc ggcatgttcg
     2461 gtgtgcagca gaaaccccac gacgagcgct acgacgtcgc tgacgactgg attcatctga
     2521 ccaaacgatt gtggacggaa actcaggagt tcgaccatca gggtccctat ttctcagcac
     2581 ctggcgccta ctccgagccc aagcccgtgc aggcgcctca ccctctgatc atgagcgccg
     2641 ggaacagccc cagaggtctg gcattcgccg ccgagcacgc agacctcaac ttcgtcgtcg
     2701 cggccgatct cgacaaggca ggggaaagtg ccagcgcggt ccgggcgaag gcagccgagc
     2761 aggggcgttc catcaaggta ttcggccagg gttacatcgt ttgccgttcc agcgaggccg
     2821 aagccagggc agcccgggac cgctacgtcc acgagctcgg cgactgggaa ggcgtccgca
     2881 acctgctcga cgtcctcatc cccaactctc aaagcgcgct gggcgatgag tgggaggcca
     2941 tggccgccaa catgatcgcc ggctacggcg cgcttccgct ggtaggcacg ccggatcagg
     3001 tcgttgacgg catgcaggcc ttcagcgacg cagggctcga tgggatgacg ctcagctggc
     3061 tcgactatcc agccggcatc gagcaattcg aaaaggagct ccgaccccgg ctcgtcgagg
     3121 ccgggttgcg cgcccgctaa cccacctgtg cagcgatctc ggcgatgggc tcgagagcta
     3181 gcgcccggtg cggcggcggc aggtaaaaaa tcagcaatcc caggcccgcc tcgcggaagg
     3241 ccgtgacgcg ccggcgcagg tcgtccagat cgtccagatc taccggctga tgggcggacg
     3301 tcatgatctc gcctgggtct cggcctacgg cccgacactg atcccacagc acgtcccgct
     3361 tcgcaacaaa tccgtcgacg tcgaaccccg ggtagttcca gtgctgcgcc cagcgcacga
     3421 cgttggggaa cgtacgccgc tcgccgttgc caccaatgca gatgggcggg tgcggctgct
     3481 gaagcggcgg cggattgcag cgggcggcgg tcagcgtgaa gtatcgtccc gcaaaatccg
     3541 tcgtcggaac gctcagcatg ccgtgaatga ccgcacaggc ctcatcgaaa cggtcgaagc
     3601 gctcagtcag ggttcccagc tcgattccgt aggcgtcgca ctcctcctcg ttccatcctg
     3661 ccccgagccc gacttccagc cgaccgtcgg agatgatgtc gagggaggcg atcatgttgg
     3721 ccagcaccgc gggatggcga tagacgacgc cgctcaccat gcagccgatg cgtatcctct
     3781 cggtcgcctg agccagggcg gtgagcgtgg tccacgcttc catgcagggc cccgaggtgt
     3841 ccgggacgtt gatggggtaa aagtggtcga agttccaggc ggagtggaat acctccatcc
     3901 catcggccgc ccgccagacg tcgagcatgt catgccaggt agtgagctgc ggggaggtct
     3961 tgattccgta aagcatcttt caaccccctt cccgatccga tgacgacggg gcagatgata
     4021 gaaccgccga cgaagaatta caccgtgcgt cccgatccgg cgcgaaagcg aagtccatca
     4081 caggcttggg cccgcaaaag ggctaccctt cgcgctgact acctcgcctg aaagtgcagg
     4141 gaagccacta tgtcattcaa acgtgcgctg gtcgtcgacg attcgaaatc cgcgcggctt
     4201 tcgctgaaaa agctgctcga gcagcaccac ctggaggtcg acgaggcctt ttccggcgaa
     4261 gaagcgctcg acttcatcaa ggaccactcc gtcgacgtca tcttcatgga ccacaccatg
     4321 cccggcatgg atggtctcga agcggtctct gccatcaaga gcaatccgaa aacggccacc
     4381 attcccgtca tgatgtacac cacccaggaa ggggaggtct acgtcggcca ggcccgcgct
     4441 ctcggcgcgg tggaagtcct gccgaagcag gtgcaacccg gcgtgctgtt cgagatgctc
     4501 gagaagctgg gcctggtgac cgaccgcagg accgaggacc a
//
LOCUS       DBNHLM010000299         3115 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_342692, whole genome shotgun sequence.
ACCESSION   DBNHLM010000299 DBNHLM010000000
VERSION     DBNHLM010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_342692"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /gene="glnL"
                     /locus_tag="AC16QF_13880"
     CDS             complement(<1..1047)
                     /gene="glnL"
                     /locus_tag="AC16QF_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HIR6449699.1"
                     /translation="MTQTHGNTAYNMPSLAKSDILDQLTGAVIVCDGSLRVCYMNPAA
                     ESLLNTSEHQSTGVQLAELLFDAAASPFADLAGIFTTEQSITKRAAQFQLRDGTEVIA
                     DLTASLEPNSGHLVIELQPLNRLLRINRDEHSFFSQQTTRTLIRGLAHEVKNPLGGLR
                     GAAQLLQRELDGSELTEYTRIIIEEADRLTQLVDNMLGSNRQPEYADVNIHELLEHVV
                     RLVDAELGGRVKFARDYDPSLPPIRADRAQLVQALINIVRNAGQALGETANPSITLRT
                     RAVRQFTIGSTRHRVVLQIEIADNGPGIPEGMVDQIWFPMISGRADGTGLGLAITQSI
                     VGHHNGIIECVSRPG"
     gene            complement(1304..1921)
                     /locus_tag="AC16QF_13885"
     CDS             complement(1304..1921)
                     /locus_tag="AC16QF_13885"
                     /inference="COORDINATES: protein motif:HMM:NF024901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR6449700.1"
                     /translation="MQLLATLKRIAFLSIISTPWLMLSTQSMAGTTIYKTVDEDGNVV
                     FTDTPPRADGPSETITLTSPNSFDPAEAGTEGSGRQLWIVDPDAEGEASGEFYGALTV
                     TSPADDESIRENAGNVTVTVKLEPPNLQPGHRIRLLLDGSPTGGTNTTGRFALSNVDR
                     GTHSLKAEVVDDAGKLIYAGPSSTFHLQRYSVLNAPKRPVNPINN"
     gene            complement(2035..>3115)
                     /gene="glnA"
                     /locus_tag="AC16QF_13890"
     CDS             complement(2035..>3115)
                     /gene="glnA"
                     /locus_tag="AC16QF_13890"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR6449701.1"
                     /translation="EAYLTSTGIGDTAYFGPENELFVFDDVRWKVEMSGASYSIGAEE
                     AAWESHSEFEGGNLGHRPSVKGGYFPVPPVDSAADLRSAMCSAMMAMGLDVEVHHHEV
                     ATACQNEIGARFNTLVRKADEVQIYKYCVHNVADAYGKTATFMPKPLVGDNGNGMHVH
                     QSIAKGDTNVFHGDEYAGLSKTALYYIGGIIKHARAINAFANASTNSYKRLVPGFEAP
                     VLLAYSARNRSASIRVPYIQGGNPRAYRVEVRFPDSTGNPYLTFAAMLMAGLDGIQNE
                     IHPGDSFDKDLYDLPAEELEGVPTVCSSLEQALQALDEDRDFLKAGGVFSDGMIDGYL
                     ALKQQEVDYLNSTTHPAEFEMYYSV"
BASE COUNT          526 a          917 c         1037 g          635 t
ORIGIN      
        1 cccggggcgg ctgacgcact cgatgatccc gttgtgatgg ccaacgatgg attgggtaat
       61 ggccagcccc agaccggtgc cgtcggctcg cccgctgatc atcgggaacc agatctggtc
      121 caccatgcct tcgggtattc cgggtccgtt gtcggctatc tcgatctgca acacgacccg
      181 atgccgggta gagccgatgg tgaactgcct gactgccctg gttctcagcg tgatggaggg
      241 gttggccgtc tcacccaggg cctggccggc gtttctgaca atgttgatca gcgcctggac
      301 gagctgcgcg cgatcggccc gaatcggcgg caggctgggg tcgtagtccc tggcaaattt
      361 gacccggccg ccgagctccg catccaccag ccgcaccacg tgctcgagaa gctcgtgaat
      421 gttcacgtcc gcgtactcgg gctgacggtt cgatcccagc atgttgtcca cgagctgggt
      481 gaggcggtcc gcctcctcga tgatgatgcg cgtgtactcg gtcagctcgc tgccgtcgag
      541 ctcgcgctgg agcagctggg cagcgccccg caggccgccg agcgggttct tcacctcgtg
      601 agcgagcccc ctgatcagag tacgggtagt ctgctgggag aagaacgagt gctcgtcgcg
      661 gttgatccga agcaggcggt tcaagggctg cagctcgatg accaggtgac cggagttcgg
      721 ctccagggag gcggtcaggt cggcaatcac ctcggtgccg tcgcgcagct ggaattgcgc
      781 cgctcttttg gtgatggact gctctgtggt gaagatgccc gccagatccg cgaacggcga
      841 agcggcggcg tcgaagagca gttccgcgag ctgcaccccg gtgctctgat gctcgctcgt
      901 gttcagcaga gactccgcgg cggggttcat gtagcagacc ctcagagagc catcgcagac
      961 gatcaccgcg cccgttagct ggtccaggat gtcggatttg gccagagacg gcatgttgta
     1021 ggcagtgttc ccgtgtgtct gagtcatggg gcgttgacgg aatgcgtgct cctgacgcaa
     1081 gaaacggtcc aactcctcgc gatcaggctg caagacgtga gtgagtattt acttcggtct
     1141 cattgccgga ataaaaggcc gatcagggcg ggcaccccct tgcgttagcg gcgccgtgcc
     1201 ccaggcagta tgcaccaata cggtgcaaag gtcgattgtg gtgcggccgg ttgacggccg
     1261 ccctcgcgat ccggcgtggt cgaatgggcc ctgcggtgtc gggtcagttg ttgatcgggt
     1321 tcaccgggcg cttgggcgcg ttgagaaccg aatagcgctg caggtggaag gtggacgacg
     1381 ggcccgcgta gatcagcttt ccggcgtcgt ccacgacctc tgccttcagc gagtgcgtac
     1441 cgcggtccac gttgctgagg gcgaaacgcc cggtcgtgtt ggtgccgccc gtgggggagc
     1501 catcgagcag gaggcgtatt cggtgaccag gctgcaggtt cggcggctcc agcttcaccg
     1561 taaccgtcac gtttcctgcg ttttcccgga tgctctcgtc gtctgctggg gaagtgaccg
     1621 tcaatgcccc gtagaactcg ccagaggctt ccccttcggc gtctggatca actatccaga
     1681 gctggcggcc ggagccctcg gtgcctgctt ctgcgggatc gaaagagttg ggggatgtca
     1741 gggtgatcgt ctcggaaggc ccatcggctc tgggcggcgt gtccgtgaac accacgttgc
     1801 cgtcctcgtc gacggttttg tagatcgtcg ttccggccat ggactgcgtg ctcagcatca
     1861 gccagggcgt ggatatgatg ctcagaaatg caattctttt cagtgttgcc agtagctgca
     1921 tgttgcccgc cttgggttgg attcagccat tttggaccac gccgaggcaa aaaaaagccc
     1981 caattcgtaa acgagttggg gctttgccgc gggcgaaacc cgcgcgaggg cgtcttacac
     2041 gctgtaatac atctcgaact cagcggggtg ggtcgtggaa ttcaggtagt ccacttcctg
     2101 ctgcttgagg gcgagatagc cgtcgatcat gccgtcggag aacacgccgc cggccttgag
     2161 gaagtcgcgg tcctcgtcga gagcctgcag cgcctgctcc aaagaagagc agacggtcgg
     2221 cacgccctcc agctcttcgg cgggcaggtc atagagatcc ttgtcgaacg agtcgccagg
     2281 atggatctcg ttctggatgc cgtcgagccc ggccatgagc atggcggcga aggtcaggta
     2341 cgggtttccg gtgctgtccg ggaagcggac ttcgacccga taggcccggg ggtttccgcc
     2401 ctgaatgtag ggaacccgga tcgatgcgga tcggttgcgg gcggagtaag ccaggagcac
     2461 gggggcctcg aagcctggca ccagccgctt gtaggagttg gtgctggcat tcgcaaacgc
     2521 gttgatggcc cttgcatgct tgatgatccc gccgatgtag tacagagccg tctttgagag
     2581 ccccgcgtac tcgtcgccgt ggaatacgtt ggtgtccccc ttggcgatgg actggtgaac
     2641 gtgcatgccg ttgccgttgt cgccaacgag cggcttgggc atgaaggtcg ccgtcttgcc
     2701 gtaagcgtcc gccacgttgt gcacgcaata tttgtatatc tgcacctcgt cggctttacg
     2761 caccagagtg ttgaagcgtg cgccgatctc gttctgacag gcggttgcca cttcgtggtg
     2821 gtgcacctcg acgtccaggc ccatggccat catcgccgag cacatggccg agcgcagatc
     2881 ggcggcggaa tccaccgggg gcacggggaa gtagccgccc ttcacggacg ggcggtggcc
     2941 cagattgcca ccttcgaact cggaatggga ttcccaggct gcctcctcag cgccgatgct
     3001 gtaggacgcg ccgctcattt ccaccttcca gcgcacgtcg tcgaacacga ataactcgtt
     3061 ctcgggcccg aagtaggcgg tatcgccgat tccggtggaa gtcaggtaag cctcc
//
LOCUS       DBNHLM010000300         2256 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1480803, whole genome shotgun sequence.
ACCESSION   DBNHLM010000300 DBNHLM010000000
VERSION     DBNHLM010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1480803"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="AC16QF_13895"
     CDS             complement(<1..936)
                     /locus_tag="AC16QF_13895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system-associated ATPase"
                     /protein_id="HIR6449702.1"
                     /translation="MYEEQFGFSAKPFQLAPDAKFYYPSAKHSRALAFLEYGLDQGDG
                     FIVVTGDVGTGKTTVLQTLLNKIDTRRLLVQTIVTTQLKEDDLLQLVASSFGLKVADQ
                     SKAMILQELERLFVQQRRRNRRVLIIVDEAQNLPPASVEELRMLSNFHLDGQPLLQVF
                     LVGQQEFRETLLGEGFEQLRQRVIATYHLEPLDLNEVQTYIEHRLKQVGWKGIPQFGT
                     GAFAAIYEFTGGVPRRINNLCDRLLLFAYLEDLNSIDAKAVESVAEEIGAEFLAGAPE
                     RMLKSGDLVADAVPEPIAAGPRSDLSDVLQRVAALE"
     gene            complement(944..1747)
                     /locus_tag="AC16QF_13900"
     CDS             complement(944..1747)
                     /locus_tag="AC16QF_13900"
                     /inference="COORDINATES: protein motif:HMM:NF025001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6449703.1"
                     /translation="MSGSPGAARRFDVWKPEPRPGVDLSSLGPRGYLTPSSGRGQLAQ
                     EMRRIKRPLVLNAQKQRSTGATDKPPANIIMVTSAVPEEGKSFIAINLAVSLAAEVDR
                     RVLLVDADVAMRDITRDFDLEGKPGVAELLAESGSRYEDYVLTTSVPGLSILPAGAPV
                     DHVDELFASRRMEALIKLLAEEDPERIVLFDTSPLLRTTESTVLARRMGQVVLVVEAN
                     KTSQSAVTKAVGLLEGCANVSLLLNKASRTESSDYGYGYSYGADLETKT"
BASE COUNT          418 a          708 c          676 g          454 t
ORIGIN      
        1 ttcgagggcc gcaacgcgct gaagcacgtc gctcagatcc gatctaggac ccgccgcgat
       61 gggctccggc accgcgtccg ctaccagatc gcccgacttg agcatccgtt ccggcgcgcc
      121 ggcgagaaat tcagcaccga tctcttctgc aacgctttcc accgccttgg catcgatgga
      181 gttcaggtct tcgagatagg caaagagcag aagccggtca cacagattgt tgattcgacg
      241 tgggacgccg ccggtgaact cgtagatcgc agcaaaagcg ccggtcccga attgcggaat
      301 gcccttccac cccacctgct tcaggcggtg ctcgatgtag gtctgaacct cgttcagatc
      361 caggggctcg aggtgatagg tcgcgatgac gcgctggcga agctgctcga atccttcgcc
      421 cagcaacgtc tcgcggaact cctgctgccc gaccaggaag acctgcaaca gcggctggcc
      481 gtccaagtga aaattggaga gcatgcgaag ctcttccacg gaagccggcg gcaggttctg
      541 cgcttcgtcg acgatgatga gtacccggcg attgcgccgg cgctgctgaa cgaagagcct
      601 ttcgagctcc tgtaagatca tcgctttgct ctggtccgcc accttcagcc cgaagctcga
      661 agcgaccagc tgaaggagat cgtcttcctt gagctgggtg gtgacgatgg tctgcaccag
      721 cagccgccgg gtgtcgattt tgttcagcag cgtctgcagg acggtggtct tgccggtgcc
      781 aacgtccccc gtgacaacga tgaaaccgtc tccctggtcc agcccgtact ccaggaaggc
      841 gagcgcccgg ctatgcttgg cgctggggta atagaacttc gcgtccggcg cgagctggaa
      901 gggtttggct gaaaatccga attgctcttc gtacatagtt aaatcaggtc tttgtttcca
      961 gatccgcccc gtagctatag ccataaccgt agtcgctcga ctcggtccgg ctggccttgt
     1021 tcaggagcag gctcacgttc gcgcatccct ccagcaaacc caccgccttc gttacggcgg
     1081 attgagaagt tttgttcgct tcgacgacca gcaccacctg ccccatgcgc cgcgcgagga
     1141 ccgtggattc cgtggtgcga agcagcggag aggtatcgaa cagcacgatg cgctccggat
     1201 cttcctcagc gagcagcttg atgagggcct ccatgcgccg gctggcgaac agctcgtcca
     1261 catgatccac cggagcgccg gccggcagga ttgaaagacc cggcacggag gtggtgagca
     1321 cgtaatcttc gtagcggctg cccgactcgg ccaggagctc ggcaacgccc ggtttgcctt
     1381 cgagatcgaa atctcgggtg atgtcgcgca tggccacgtc ggcatccacc agcagcacgc
     1441 gacggtccac ttcagcggcg aggctcaccg ccagattgat ggcgatgaag gattttcctt
     1501 cttccggcac cgcgctggtt accatgatga tgttggccgg tggcttgtcg gtagcgcccg
     1561 tgcttcgctg cttctgcgcg ttgagcacca acggccgctt gatccgacgc atttcctggg
     1621 ccaactgccc gcgccccgat gacggcgtta gatatcctcg ggggccgaga ctcgagagat
     1681 ccacgcccgg cctcggctcg ggcttccaga catcgaagcg tcgtgctgca ccgggcgagc
     1741 cagacactgc atctgctacc tttgccttcg attcattcac ccgagattca ttgagatcta
     1801 ccgcagttgg atcgactcct agcccgggtt catcaggggc gggcaggaca cggtcggaaa
     1861 ccaccgggat gtgctgttca gaggccggcc ccgaagcatc agcttcagcg tctgggcgcc
     1921 ccgcggctgc ggccacgaac gggtcgacgt ccgcctgccc gaggtcttca ctctcgggtt
     1981 ccggtttttt gtcctgggcc gaccgcgaag caagctttgc ggcggctttc tcaattaagc
     2041 tcatggacct cggggcgcgt tgcgcaatga tcgctgttag aagtgtatcc aaccggcctc
     2101 acggcctgtg taagatgttg tatcgcatac cgtcatgacc ggccgcaacc cccaaatggt
     2161 cagtccaact gacgtttttg aattgccgaa ccaggtatca gcagcgcggc gaccggttcg
     2221 ggcataatgc gctccgttcg caccgagaac gccgta
//
LOCUS       DBNHLM010000301         8375 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1001537, whole genome shotgun sequence.
ACCESSION   DBNHLM010000301 DBNHLM010000000
VERSION     DBNHLM010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8375
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1001537"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /gene="aspS"
                     /locus_tag="AC16QF_13905"
     CDS             complement(<1..1622)
                     /gene="aspS"
                     /locus_tag="AC16QF_13905"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR6449704.1"
                     /translation="MRSHYCGELNAGNVGETVELYGWVNRRRDHGGVIFLDVRDRTGI
                     AQVVFDPDAPESFAVADSVRNEYVLRLTGRVRPRPEGTVNAEMATGEVEVLGAELEVL
                     NVSETPPFQLDEHSEAGEDVRLKYRYLDLRRPEMQSRIFARARIASAVRRFLEDEGYW
                     EVETPTLTRATPEGARDYLVPSRTHPGEFFALPQSPQVFKQLLMISGMDRYFQIARCY
                     RDEDLRHDRQPEFTQIDLEASFVTAEDVMALTERMLKQVFSQALDVELPEFPVLTWAE
                     AMSRYGSDKPDLRNPLELVDVADLMRDVEFKVFNAPANDPGGRVVALRAPGGGSLSRK
                     VIDDYADFVARYGAKGLAYIKVNDLGAGRAGLQSPILKFLPDEVVSEVLARVGAGDND
                     VIFFGADTAGVVADAMGALRDALAGDLDLLEDRWAPCWVINWPMFEMGRDGVPTPHHH
                     PFTRPTCTPEEMLAEPMVAEAAAYDVVLNGYELGGGSLRIHDREMQQAVFDVLRLGEE
                     AEVRFGFLLDALKFGCPPHGGIALGLDRLVML"
     gene            complement(1816..2028)
                     /locus_tag="AC16QF_13910"
                     /pseudo
     CDS             complement(1816..2028)
                     /locus_tag="AC16QF_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017139808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
     gene            2327..2743
                     /locus_tag="AC16QF_13915"
     CDS             2327..2743
                     /locus_tag="AC16QF_13915"
                     /inference="COORDINATES: protein motif:HMM:NF012442.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR6449705.1"
                     /translation="MARKKAPAKKKAPAKKKAPAKKKAAAKAAPQRQAPALKAKMSKT
                     AMLSEIAEKTNLTRGQVGSVLDELEVLIERHVKKRSVGEFTLPGLLKIRSVKRPASKA
                     RMGRNPATGEEIMIKAKPASIRVRVTPLKKLKDMVG"
     gene            2875..4575
                     /locus_tag="AC16QF_13920"
     CDS             2875..4575
                     /locus_tag="AC16QF_13920"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR6449706.1"
                     /translation="MRQSMLLIPTMKENPADADVASHRLMLRAGLIRQVASGLYTWLP
                     LGLRVIRRIESIIREEMNQSGAQEVLMPVVQPAELWEESGRWQKMGPEMLRMRDRHER
                     DFCLAPTHEEVITDLFRREVHSYRQMPCNFYQIQTKFRDEIRPRFGVMRAREFTMKDG
                     YSFHLDQACFDDTYRTMYDCYSRILTRMELEFRAVEADTGNIGGANSHEFHVLAESGE
                     DTIAYATHGPYAANLDKARAAPPAAGRSPGDELRKVATPGVETIEAVARFLEVSPAQT
                     LKTLLVRGSSEPVVALILRGDHDLNELKAEKLDEVADPLSFATEAEIQAATGCRPGSL
                     GPIGLDVPAYVDVEAAALTNFVCGANEDGFHYTGANWDRDQTLDTARVVDFRNVVAGD
                     PAPDGQGTLQFLRGIEVGHIFQLGSVYSEPLGAAVLGPDGASITPIMGCYGMGVTRLV
                     AAIIEQNHDEVGIIWPDPVAPYDLHIIALNYGKSETVREAADRLYEACLSDGMEVLLD
                     DRDERPGVKFAEADLIGIPRRITVGERGLKDQTVEYRRRRDSENEVVTLDGMLDRLRS
                     "
     gene            4578..5225
                     /locus_tag="AC16QF_13925"
     CDS             4578..5225
                     /locus_tag="AC16QF_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR6449707.1"
                     /translation="MGRFGLSVALLLCAICPLRTPSALADVRIDPVLLEKLAVSMADR
                     YEHTDRFDAEVWLVTSERRLARYMDDHDERLALLQTVYREADRNALDADLVLAVMQVE
                     SAFDRYAISRVGAQGLMQVMPFWRNEIGRPRDNLTDMETNIRYGTTILAHYIEVSDGD
                     LVDALARYNGSRGRLKYPEKVVGAWRRVWRHKSSDELPDLVSTCATYALHACRYR"
     gene            complement(5272..5763)
                     /locus_tag="AC16QF_13930"
     CDS             complement(5272..5763)
                     /locus_tag="AC16QF_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449708.1"
                     /translation="MLRKLAIRDVLIVAVATAGWLGLLPLSEGRPLISDSLALLLGLG
                     VTVSAYLLHEWGHALAGLVVGSRIHAPASLRSVSLFSFDAEANSKTQFAAMSIGGFVV
                     TAAAMGFVYFVLPDGYMASRVARGGVTVLASITVFIEVPLLLYGLLARRLPGVVAVFR
                     PET"
     gene            complement(5766..6245)
                     /locus_tag="AC16QF_13935"
     CDS             complement(5766..6245)
                     /locus_tag="AC16QF_13935"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HIR6449709.1"
                     /translation="MKAKTSSLLLAGALFLAGCDFLDQADEPPLALADGTTMKVDDWH
                     GRWLLINYWAEWCAPCRKEIPELNRLQAEVGEERLLVIGVNFDGLTGQRLTELIEKMD
                     VRFPVLVEDPRGRWDVEQPTVLPSTLVIAPNGRLKEVLVGPHTFESFSRALGLTAST"
     gene            complement(6242..7471)
                     /locus_tag="AC16QF_13940"
     CDS             complement(6242..7471)
                     /locus_tag="AC16QF_13940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HIR6449710.1"
                     /translation="MLEATYDQFQERVVVPLRWYVGQLGQQFLIHDCMTRAGALTYTT
                     LFAVVPMMTVAYAMFSVLPEFETVGERIQEYIFQNFVPDSSAVVQERLLEFSARARQL
                     TAAGFLVLFITAFMMLVSVEKTFNTIWSVAEPRRGLQRFLLYWGVLSLGPPLIAAGIF
                     ISLYLISLPLIADLDTFGLDSAVLDYLPSVLTAAGLTVLFLAVPNCRVPFHHALAGGV
                     LTMFAFELAKKVFRLTVANSSMEPIYGTFAAVPLFLIWLYLVWVLILSGAIFVRTLSL
                     KRDRPVDNPEPAVVKCSRVVKFLHAAHLEGRSVSDSEINDQVPLARAERERVFEALLA
                     LKLVRETEDGGWMLGRSLKTLTLWDLYRALPDGVDAARLGTVKDMDGVVEPLKAFVQF
                     GSNQMAVSLDSVFGKSS"
     gene            7534..7884
                     /gene="arsC"
                     /locus_tag="AC16QF_13945"
     CDS             7534..7884
                     /gene="arsC"
                     /locus_tag="AC16QF_13945"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252402.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HIR6449711.1"
                     /translation="MREVTIYHNPRCSKSRATLALLEEHGVTPNVVEYLSTPPDAATL
                     KRLLDLLGLSPRELMRRKETVYSDLGLDDDALGEDALIEAMIANPILIERPIVVAGDR
                     AVIGRPPENVLGLV"
     gene            7888..>8375
                     /locus_tag="AC16QF_13950"
     CDS             7888..>8375
                     /locus_tag="AC16QF_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIR6449712.1"
                     /translation="MTGPVHPYVLILYYSRTGGTAHLARYVARGVQAQGDFEARVRTV
                     PPVSTTVEQTEPVVPEHGAVYCTKADLAGCGALAIGSPTRFGNMAAPLKHFLDDTADL
                     WMQHALVGKPAGVFCSTASLHGGQESTLLSMMIPLLHHGMLVSGVPYSEPALNRTARG
                     GGP"
BASE COUNT         1628 a         2741 c         2616 g         1390 t
ORIGIN      
        1 atgagcatca ccagacgatc gagcccgagc gcgatgcctc cgtgaggcgg acacccgaac
       61 ttcagcgcat cgagcagaaa accgaagcgc acctccgctt cctcgccgag cctgagaacg
      121 tcgaaaacgg cctgctgcat ttctcgatca tgaatccgga gggagccccc gcccagctcg
      181 tagccgttga gcaccacgtc gtatgcggca gcttcggcga ccatgggctc ggccagcatc
      241 tcctcggggg tgcacgtagg ccgggtgaaa ggatggtggt gcggcgtcgg gacgccgtcg
      301 cgccccatct cgaacatggg ccagttgatc acccagcacg gcgcccatct gtcctccaga
      361 aggtcgagat cgccggccag cgcgtcgcgc agcgcgccca tggcgtcggc aaccacgccg
      421 gcggtgtcgg cgccgaagaa gatcacatcg ttgtcaccgg ctccgacgcg cgcgagcacc
      481 tcggacacca cctcgtcggg gaggaacttg aggatcggcg actgcaggcc ggccctgccg
      541 gctccgaggt cgttcacctt gatgtaggcg agccccttcg cgccgtagcg cgcgacgaaa
      601 tcggcatagt cgtcgatgac cttgcggctg agcgagcctc cgccgggggc ccgtagcgcc
      661 accacacgac cgccagggtc gttggccgga gcgttgaaga ccttgaactc gacgtcgcgc
      721 atcaggtcgg cgacgtcgac gagttccagc gggtttctca ggtccggctt gtcgctgccg
      781 taacggctca tggcttcggc ccaggtgagc acgggaaact cgggcagctc gacgtcgagc
      841 gcctggctga acacctgctt gagcatgcgc tcggtcaatg ccatgacgtc ctcggccgtg
      901 acgaacgagg cctcgaggtc gatctgcgtg aactcgggct gcctgtcgtg gcgaagatcc
      961 tcgtcccggt aacagcgggc gatctggaag tagcgatcca tgcccgagat catgagcagc
     1021 tgcttgaaca cctgcggcga ctgcggaagc gcgaagaact cgcccgggtg ggtacggctg
     1081 ggaaccagat aatcgcgcgc gccctcgggg gtggcgcgcg tcagcgtcgg ggtttcgact
     1141 tcccagtacc cttcgtcctc caggaagcgg cggaccgcgc tggcaatgcg tgcccgcgcg
     1201 aaaatgcggc tctgcatttc cgggcggcgc aggtcgaggt agcggtactt gagacggacg
     1261 tcctcgcccg cttccgaatg ctcgtcgagc tgaaacggcg gggtctcgga gacgttgagc
     1321 acttcgagtt ccgctccgag gacctccacc tcgccggtgg ccatctcggc attcaccgtg
     1381 ccctcgggtc gcggccggac ccgcccggtc agccggagaa cgtactcgtt gcgaacgctg
     1441 tcggccactg cgaagctctc cggggcatcc gggtcgaaca ccacctgggc aatccccgtc
     1501 cggtcacgaa catcgagaaa gatcacgccg ccatgatcac ggcggcgatt cacccagccg
     1561 taaagctcta ccgtctcgcc aacgttgccg gcgttgagtt ccccgcagta gtgactacgc
     1621 atggctgctt cctgctcgca tgaatccaga gcgtcccttg gttccgatgg gttgtgctaa
     1681 gacttattcc gatttgctcg tggtcgagcc cgaggtggtc gagctgccgg gcttggcacc
     1741 gccatcggcc ggcttgctcg agctcgctga cgacgactcc gaagtggcgc ccgcgctcga
     1801 cttgtccgcg ctttcctttt tgtcttcggt gaggttgcgc ttgtttccgg acttgaaatc
     1861 ggtctcgtac cagccgccac ccttcaaccg gaatccgggc gccgacacct tcttgaacag
     1921 agcgtcctgc gcacactcgg gacacgtctg caaaggcgct tcgcttatct tctggatgac
     1981 ttccagctcg tggccgcagt tcgagcagcg atattcgtag atgggcatga tcgaaaatct
     2041 ctgattctgc aaacgtcagt tgagcgagcg gcgcatcatc ttaaaaaaac cagcaaaaat
     2101 acagcgatat cgcaggcttt ttgttgcttg gccgcttccg atcggctata aaccgcggcc
     2161 atcgagagtg tcggagtgac ggcgagctag ccaggaatat caggcgtttc cgtgcaagcc
     2221 gcgatctcag ccgccgcagt ctttcggtaa atggggaccc gctacgcaac cgggtatcgg
     2281 agagcgaagc tggttcagct aaataatcca gctcaaggaa tattgaatgg cacgcaaaaa
     2341 ggcccccgcc aagaaaaagg ctccggcaaa gaagaaggca cccgcaaaga aaaaggctgc
     2401 agcgaaggct gcaccgcagc gccaggcgcc tgctctgaag gccaagatgt ccaagacggc
     2461 aatgctctcg gagatcgcag agaagaccaa cctgacccgc ggccaggtgg gctcggttct
     2521 cgatgagctc gaagtcctca tcgagcgcca cgtcaagaag cgttcggtcg gtgagttcac
     2581 tctgccgggc ctgctgaaga tccgttccgt caagcgcccc gcgagcaaag cgcgaatggg
     2641 ccgcaacccg gccaccggcg aggagatcat gatcaaggcc aagccagcga gcatccgtgt
     2701 tcgggtgacc ccgctgaaga agctcaagga catggtcggc tagatcgtgg ctccgtgacc
     2761 gaacgctggc gatgcgggcg gtcatcggca gaaaaggggc cttgcgcccc ttttttgctg
     2821 caatttcgct aatctaggcc aaacgtcctg caatcgatcc agagggcact gcacatgcgg
     2881 caatccatgc tgctcattcc caccatgaag gaaaatccgg ccgacgccga cgtggccagt
     2941 catcgcctca tgcttcgggc cggcctcatc cgacaggtcg cctccggtct ttacacctgg
     3001 ttgcccctgg ggctgcgggt gatccgtcgg atcgagtcga tcattcgtga ggagatgaat
     3061 caaagtggcg cccaggaggt gctgatgccg gtggtgcaac cggcagagct gtgggaggaa
     3121 tccggacgct ggcagaagat gggacccgag atgctgcgca tgcgggatcg tcacgagcgc
     3181 gacttctgcc tggcgcccac tcacgaggaa gtgatcaccg atctgttccg gcgcgaggta
     3241 cacagctacc ggcagatgcc gtgcaacttc taccagatac agaccaagtt ccgtgacgag
     3301 atccgcccca gattcggcgt gatgagagcg cgggagttca ccatgaagga cggctactcg
     3361 ttccatctgg accaggcctg cttcgacgat acgtatcgaa cgatgtacga ctgttactcc
     3421 cgtatcctga cgcgaatgga gttggagttc cgcgccgtgg aagcagatac cggcaacatc
     3481 ggcggcgcga attcccatga gtttcacgtc ctcgcggagt cgggcgagga caccatcgcc
     3541 tacgccaccc acggaccgta cgcggccaac ctggacaagg cgcgcgccgc gccgccggca
     3601 gccggccgtt cgccgggtga tgagctccgg aaggttgcca ccccgggcgt cgagaccatc
     3661 gaggcggtcg cccgttttct ggaggtctcg ccggcccaga ccctgaagac cctgctggtc
     3721 cgtggttctt cggaacccgt ggtcgcgctg atcctgcgcg gagaccacga tctgaacgag
     3781 ctcaaggcgg agaagctgga cgaggtggct gacccgctca gcttcgccac ggaggctgag
     3841 atacaggcag ccacggggtg caggcccgga tccctcggac cgatcgggct cgacgtgccg
     3901 gcctatgtgg atgtggaggc ggctgccctg accaacttcg tttgcggcgc caacgaagac
     3961 ggcttccact acaccggggc gaactgggat cgcgaccaga ctctggatac agcccgcgtg
     4021 gtggacttcc ggaacgtggt ggccggcgac ccggcgcccg acggccaggg cactctgcag
     4081 ttcctgcgcg gtatcgaggt cggccacatc ttccagctcg gcagcgtcta cagcgagccg
     4141 ctgggcgccg cggtgctggg accggacggc gcgagcatca cacccatcat gggctgctac
     4201 ggaatgggcg tgacgcgcct ggtcgcggcc atcatcgagc aaaaccatga cgaggtcggc
     4261 attatctggc ccgatcccgt cgcaccctac gaccttcaca tcattgcgct caattacggc
     4321 aagtcggaga cggttcgcga agcggccgac aggctctacg aagcctgctt gagtgacggc
     4381 atggaggtac tcctcgatga tcgggacgag aggccgggcg tgaaattcgc cgaggcggat
     4441 ctcatcggaa tcccacggcg catcaccgtg ggcgaacgcg gactgaagga ccagaccgtc
     4501 gagtatcgtc gccgccgcga ctcggagaac gaggtggtga cgcttgacgg gatgctggac
     4561 cggctgagga gctgatcttg ggccgcttcg gcttgtctgt ggcgttgctc ctgtgcgcca
     4621 tctgcccgct gcggacgcca tctgcgctgg cagatgtacg catcgacccc gtcttgctgg
     4681 aaaagctcgc cgtttccatg gccgaccgct acgagcacac ggaccgtttc gacgcggaag
     4741 tctggctggt cacatccgag cgacgcctcg ccagatacat ggacgatcac gacgagcggc
     4801 tggcactgct tcagaccgtc taccgcgaag ccgacagaaa cgcgctggat gcggatctgg
     4861 tgcttgccgt catgcaggtc gagagcgctt tcgaccgcta cgcgatctcg cgagtcggtg
     4921 ctcaggggct catgcaggta atgccattct ggcgaaacga gatcggccga ccccgtgaca
     4981 acctcaccga catggaaacc aacatccgct acggcacgac cattctggcg cactacatcg
     5041 aggtatcgga cggcgacctg gtagacgctc tggcacgcta caacggcagc cgcggccgcc
     5101 tgaagtatcc ggaaaaggtc gtcggcgcct ggcgccgcgt ctggcgccac aaatcgtccg
     5161 acgagctgcc cgacctcgta tccacctgcg caacctacgc cctgcacgcc tgccgctacc
     5221 gatagggcat cgtctcgatc tcgacggttc cggcaaacaa cggtccagcg gtcaggtttc
     5281 cgggcggaat accgccacga cgcctggcag gcgcctggcg agcaatccgt acaaaagcag
     5341 gggcacctcg atgaaaacgg tgatcgaggc cagcacggtc accccgcccc gagcaacacg
     5401 ggaagccata tagccgtcgg gcagcacaaa atagacaaag cccatcgcgg ccgccgtgac
     5461 cacgaaccca ccgatggaca tcgcggcgaa ctgggttttg ctgttggctt cggcgtcgaa
     5521 gctgaacagc gacacgctgc gcagcgaggc cggcgcgtgg atgcggctgc cgaccacgag
     5581 accggcgagc gcgtgacccc attcgtgcag gaggtaggca ctcaccgtca cgccgagacc
     5641 gagcaacagg gccagcgaat cgctgatgag cgggcgtcct tcggacaatg gcagcaggcc
     5701 gagccagccg gcggtcgcca ccgccacgat cagcacgtcc cggatggcga gcttgcgcaa
     5761 catcgtcacg tggacgcggt tagacccagg gcccggctga agctctcgaa ggtatgcggc
     5821 cctaccagca cctccttgag gcgaccgttg ggcgcgatga ccagcgtgct cgggagcacg
     5881 gtgggctgct ccacgtccca tctgccccga gggtcctcta caagcaccgg aaaccggacg
     5941 tccatctttt cgatgagctc cgtgagccgc tgcccggtga ggccgtcgaa gttgacgccg
     6001 atcaccagca gccgctcctc gcccacctcc gcctgcaggc gattgagctc cggaatctcc
     6061 ttgcggcacg gcgcgcacca ctccgcccag tagttgatca gcagccagcg cccgtgccag
     6121 tcgtcgacct tcatggtcgt accgtcggcc agagccagag gcggctcgtc tgcctggtcg
     6181 agaaaatcac agccggccag gaaaagcgca ccagccagca agagagagct cgtcttcgcc
     6241 ttcatgagga tttaccaaac accgaatcga ggctcaccgc catctggttg gagccgaact
     6301 ggacgaaggc cttgagcggt tcaaccacgc catccatgtc cttcaccgta ccgagccgtg
     6361 ctgcgtccac gccgtccgga agcgcacggt agagatccca aagcgtcagc gtcttcaggc
     6421 tgcgaccaag catccaaccc ccatcctccg tttccctcac cagcttgagg gcgagcagcg
     6481 cttcgaatac ccgctcccgt tcggcccggg ccagcggcac ctggtcgttg atctccgagt
     6541 cgctgacgct tcggccctcg agatgggcag cgtggagaaa cttcacgacc cgcgaacact
     6601 tgaccaccgc gggctcgggg ttgtccacgg gccggtcgcg cttgagcgac agggtgcgga
     6661 cgaaaatcgc cccgctgagg atcagcaccc agacgaggta gagccagatc aggaaaaggg
     6721 gcaccgcagc aaaggttccg tagatgggct ccatgctgga gttggcaacc gtgagcctga
     6781 ataccttctt cgccagctcg aaggcgaaca tcgtcaggac gccgccggcg agggcatggt
     6841 ggaagggaac ccggcagttg ggaaccgcga ggaagagaac ggtcagcccc gccgcggtca
     6901 gcacgctcgg caggtaatcg aggacggcgc tgtccagccc gaaagtgtcg agatcggcga
     6961 tcagaggaag ggatatgagg tacagagaaa tgaaaatccc ggcagcgatc agcggcgggc
     7021 ccagggagag aacgccccag tacagcagga atcgctgcag tccccgccgc ggctcggcta
     7081 cgctccagat ggtgttgaac gtcttctcca cggagaccag catcatgaaa gccgtgatga
     7141 acagcaccag aaagcccgcc gcggtgagct ggcgcgccct tgccgagaat tccaggaggc
     7201 gttcctggac cacggcgctg ctgtccggca cgaagttctg aaatatgtat tcctggatcc
     7261 gctcgccgac ggtctcgaac tccggcagca ctgaaaacat ggcgtacgcc accgtcatca
     7321 tcggcaccac ggcgaagagc gtggtgtagg tcaacgctcc cgcccgcgtc atgcagtcgt
     7381 ggatcaggaa ctgctgaccc agctgcccga cgtaccagcg cagcggcacg accacccgtt
     7441 cctggaactg atcatacgtc gcctccaaca ttgccagtct cccgcgcgac cggttaaatg
     7501 aaatcgccgc cagaaccgaa cattataaag accatgcgtg aagtcaccat ctaccacaat
     7561 ccgcgctgct ccaagtcgcg ggccacgctg gccctgctcg aagagcacgg ggtgacgccc
     7621 aacgtcgtcg agtacctgag cacgccgccc gacgcggcga ccctcaaacg gctgctcgac
     7681 ctgctcggac tctccccgcg agagctcatg cgccggaagg aaaccgtcta ctccgatctc
     7741 ggtctggacg acgacgccct cggcgaggat gccctcatcg aggcgatgat cgccaacccg
     7801 atactcatcg agcggcccat cgtcgtcgcc ggcgacaggg ccgtcatcgg ccggcctccc
     7861 gagaacgtcc tggggctggt ctgacccatg accggacccg tgcaccccta cgtcctgatc
     7921 ctctactaca gccgtaccgg cggaaccgcg cacctggccc gatacgtcgc gcgcggcgtt
     7981 caggcccagg gtgacttcga ggcgcgggtg cgcaccgtgc cgcccgtatc caccacggtg
     8041 gagcagaccg agcccgtggt gcccgagcat ggcgccgttt actgcacgaa ggcggacctg
     8101 gccggatgcg gcgccctggc catcggctcc cccacccgat tcggcaacat ggcggcaccg
     8161 ctgaagcact tcctcgacga tacggccgat ctgtggatgc agcacgccct ggtgggcaaa
     8221 ccggctggcg tgttctgctc gaccgcctcg ctgcacggcg gccaggaatc caccctgctg
     8281 tcgatgatga tcccgctgct ccaccacggc atgttggtct ccggcgtgcc ttacagcgag
     8341 cccgccctca accggaccgc caggggcggt gggcc
//
LOCUS       DBNHLM010000302         3445 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_343404, whole genome shotgun sequence.
ACCESSION   DBNHLM010000302 DBNHLM010000000
VERSION     DBNHLM010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_343404"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..309
                     /locus_tag="AC16QF_13955"
     CDS             4..309
                     /locus_tag="AC16QF_13955"
                     /inference="COORDINATES: protein motif:HMM:NF020474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcaB family transcriptional regulator"
                     /protein_id="HIR6449713.1"
                     /translation="MSARHVGLLARDEAEGMLHQGGRVVRRVLQSPVGYRFLGVTRRD
                     LAQGTAKALHAIEVMGEVPGDVLTGTCRAPHAPTAVDQPRENPLTDPTSLRSLPNLG"
     gene            407..799
                     /locus_tag="AC16QF_13960"
     CDS             407..799
                     /locus_tag="AC16QF_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449714.1"
                     /translation="MSATVSVSDVTTPKRLSITLDPGLVLERLILRRLADLQRKRGQD
                     WIRSLLVQGFLAESRWLRIGEPGGRQSRVPVDPAIPTTPFARWLQGAHDNGGSDQEPA
                     PRSAEAPTTPPSTSSNAKPFAHLRKVIG"
     gene            829..1845
                     /gene="parM"
                     /locus_tag="AC16QF_13965"
     CDS             829..1845
                     /gene="parM"
                     /locus_tag="AC16QF_13965"
                     /inference="COORDINATES: protein motif:HMM:NF044815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /protein_id="HIR6449715.1"
                     /translation="MADSETLKPISIGLDDGYVFTKVAMPDGRLIAIPSRARIGRSNV
                     TWLNGSEQRVFEYETDDTLFAVGEVEGEPTHFDGYPFSGLNRAIVQHALHEAALAGQS
                     VHAVSGLPVSSFYLKDGDLRLELIERKRASLKRAVQPIDGRLPAAIAFHEVIPEALAA
                     WYDHIISEANGGVQLEAERLGVPVAVIDIGGRTTDFVVVADQAVRHDSSGSLRCGLLD
                     LKRQVASSIRANFDLEELSERATDDAVRSGSVRLFGKTHDVAELVRHARRELVERLHA
                     ETQRQLGRGAELERILFVGGGAVALAEDIRDWFPNQTLAPHPAFANARGMLKYLRYVC
                     QEPT"
     gene            2055..2450
                     /locus_tag="AC16QF_13970"
     CDS             2055..2450
                     /locus_tag="AC16QF_13970"
                     /inference="COORDINATES: protein motif:HMM:NF023523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HIR6449716.1"
                     /translation="MSNEDTKYFDLYTTGIGYLNRVREVTPKEGSPFWSVTIAALRGS
                     TDDAQYSYFECRVSGKQAQEIVRQLKPAVEGKLKVLVGFTLSDLFAEAYTYKNGDKAG
                     ETGVSLKARLLRVGWAKVDGQPFYSEQAA"
     gene            2553..3065
                     /gene="radC"
                     /locus_tag="AC16QF_13975"
     CDS             2553..3065
                     /gene="radC"
                     /locus_tag="AC16QF_13975"
                     /inference="COORDINATES: protein motif:HMM:NF015935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIR6449717.1"
                     /translation="MNNQDAESFSDANRFDALKPGGLNREEKESVIKLALSVLAERHR
                     AGRALGSPEQTRSFLRLKLVDRKHEVFGALFLDNRHRVIQVVEMFQGTIDGASVHPRV
                     VVQRSLELNAAAVVFFHNHPSGVAEPSHADEAITRRLRDALALVDVRVLDHFVVAAGE
                     SVSFAERGLL"
     gene            3162..>3445
                     /locus_tag="AC16QF_13980"
     CDS             3162..>3445
                     /locus_tag="AC16QF_13980"
                     /inference="COORDINATES: protein motif:HMM:NF042459.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6573 family protein"
                     /protein_id="HIR6449718.1"
                     /translation="MSNQPSEHPFFGEVISVYTRAQALADGVLIDAGPMSREAGFRWP
                     TAITAGAWADCVAWDDADSERQAHQDQSGRLWDVLFMAAYAARANATAGS"
BASE COUNT          601 a         1123 c         1110 g          611 t
ORIGIN      
        1 gtcctgagtg cgcgccatgt ggggcttctc gcgcgcgatg aagccgaagg catgttgcac
       61 cagggcggcc gcgtcgtgcg acgtgtgctg cagagtcctg tcggctatcg cttcctcggt
      121 gtgacgcggc gcgacctcgc ccagggtacg gcgaaggcgt tgcatgctat cgaggtcatg
      181 ggcgaagtgc cgggtgacgt tctcacgggg acgtgccgcg cgccgcacgc gcccacggcc
      241 gtggatcagc ctcgagagaa tcccctcacc gatccaacaa gtctccgctc attgccgaac
      301 ctcgggtaac gcccagactc cgcggccgcg aatcgctgcg gtggccggat acgtttgctc
      361 tggtcccaga atccctcaac cgaataatca cgacatcgat catgtcgtga gcgccaccgt
      421 gtcggtttcc gatgtcacta cgccgaagag actcagcatc accctggatc cggggttggt
      481 gctggaacgg ctgatcttgc gccggctggc cgacctgcag cggaagcggg gacaggattg
      541 gattcggtcg ctgctggtgc aggggttcct cgcggagagt cgctggcttc gcatcggcga
      601 gcctggcggc aggcaaagtc gcgtgcccgt cgaccccgcc atcccaacga caccgttcgc
      661 tcgatggctg caaggcgcgc acgacaacgg cggtagcgac caggagccgg cgccgcgttc
      721 ggcggaggcg ccgacaacgc ctcccagcac cagcagcaat gccaaaccct ttgcccatct
      781 ccgcaaggtg atcggctgaa ccctgtaacc gacgagccgg aggtgaccgt ggccgacagc
      841 gagactctga aacccatcag catcggcctg gacgacggct atgtgttcac caaggttgcg
      901 atgcccgacg ggcgcctgat cgcgatcccg tcccgcgcac ggatcggtcg ctccaacgtg
      961 acctggctga acggcagcga acagcgggtg ttcgagtacg agaccgacga cacgctgttc
     1021 gccgtcggcg aggttgaagg cgagccgacc catttcgacg gctatccctt ctctggtctg
     1081 aatcgcgcca tcgtccagca cgcgttgcac gaagccgccc tggccggcca gtccgtgcac
     1141 gccgtgtctg gcttgcccgt cagcagcttc tacctgaagg acggcgacct gcggctggaa
     1201 ctcatcgagc gcaagcgggc gagcctgaag cgggccgttc agccgatcga cgggcgtttg
     1261 ccggccgcga ttgcgttcca cgaggtcatt ccggaagcgc tcgcggcctg gtacgaccac
     1321 atcatctccg aggcgaacgg cggcgtgcag ctcgaggccg agcgcctcgg cgtgcccgtc
     1381 gcggtgatcg acatcggcgg acggaccaca gatttcgtgg tggtagccga ccaggccgtg
     1441 cgccacgaca gctcggggtc tctgcgttgc gggctgctcg atctcaagcg ccaggtcgca
     1501 tcatccatcc gtgccaactt cgatctcgag gagctttccg agcgcgcgac cgacgatgct
     1561 gtgcgtagcg gcagcgtccg cctgttcggc aagacgcacg atgtcgccga gttggttcgg
     1621 catgcgcgtc gcgagctcgt cgagcggctg cacgctgaga cacagcggca gctggggcgg
     1681 ggcgccgagc tcgagcggat tctcttcgtg ggcggcggcg ccgtggcgct cgccgaggac
     1741 attcgcgact ggttcccgaa tcagaccctc gcgccgcacc ccgccttcgc caatgcgcga
     1801 gggatgctca agtacctgcg ctacgtctgt caggagccga cctgaagccg gtttcatccg
     1861 ttgtcactgc ggtctcagtg acacctgatg catcgtcggg ctgtgaaatc gatgcacaca
     1921 cccgcgccgc aaggcaacgg tgtcagccca gaagaggtcg ttcgcgacct cgactcaacc
     1981 caccctgccg ggaatgtgct cccgacaggg atggcgcgtt ctccggatcc cgttccgttt
     2041 gaaggagaaa cgtcatgtcc aacgaagata cgaagtattt cgacctctac accaccggca
     2101 tcggttatct gaatcgtgtc cgggaggtga cgccgaagga aggctcgcct ttctggagcg
     2161 tcaccatcgc tgctcttcgg ggcagcaccg acgacgccca gtacagctac ttcgagtgtc
     2221 gcgtgtccgg caagcaggcg caggagatcg tgcgtcagct caagccggcc gtcgagggca
     2281 agctcaaggt gctcgtgggg ttcacgctca gcgacctgtt cgctgaagcc tacacctaca
     2341 agaacggcga caaggccggt gagaccggag tcagcctgaa ggctcgcctg ttgcgagtcg
     2401 gctgggcgaa ggtcgacggc caaccgttct acagcgagca ggccgcctaa tccgccacta
     2461 ccgaccgggt gacttcgtca cccggtcatc ttcaacccgt cggggagcca tccctgacgg
     2521 gcggtctccc catctttcgt gaggagacct taatgaacaa tcaggatgcg gagtcgtttt
     2581 cggacgcgaa tcgtttcgat gcgctgaagc cgggcggact caaccgcgag gagaaggaat
     2641 ccgtgatcaa actggccctg tccgtgctcg ccgaacgcca tcgtgcgggt cgcgcgctgg
     2701 gcagtccgga gcagacacgg tccttccttc gactgaagct cgtcgaccgc aagcacgagg
     2761 tgttcggtgc gctgttcctc gacaaccgcc accgggtcat ccaggtggtg gagatgttcc
     2821 agggcaccat cgacggcgca tcggttcatc cacgtgtcgt cgtacagcgt tccctcgagc
     2881 tcaatgcagc cgcggtcgtc ttctttcaca accacccttc gggggtcgcc gagccgagcc
     2941 atgcagacga ggcgatcacc cggcggctgc gggacgcgtt ggcgctggtg gacgtacgag
     3001 tgctcgatca cttcgtcgtc gctgccggcg agagcgtgtc ctttgccgaa cgcgggctcc
     3061 tttgagccca tctgtcatca accatcgacc tgccgggaag gcgccccttc ccggcaggga
     3121 ggaggtgtct ctccggggtc gcaatcccaa ggagaacgac gatgagtaat caaccgagtg
     3181 aacacccgtt tttcggggag gtcatttccg tctacacccg cgcccaggcc ctggccgacg
     3241 gcgtcttgat cgatgcagga cccatgtcgc gtgaggccgg cttccgctgg ccaacagcca
     3301 tcacggctgg tgcctgggca gactgcgtag cctgggacga cgccgacagc gagcgacagg
     3361 cccatcagga tcagtccggt cgcttgtggg acgtgctgtt catggctgcc tacgctgcac
     3421 gcgccaatgc cactgcgggt tccga
//
LOCUS       DBNHLM010000303        21374 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1421941, whole genome shotgun sequence.
ACCESSION   DBNHLM010000303 DBNHLM010000000
VERSION     DBNHLM010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 21374)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21374)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21374
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1421941"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="AC16QF_13985"
     CDS             <1..960
                     /locus_tag="AC16QF_13985"
                     /inference="COORDINATES: protein motif:HMM:NF012363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIR6449719.1"
                     /translation="LIADGRASSPEDARRVQENMPAGAIRDYLYGKSLTELFAVFDNG
                     FAGMIDTPEALGDGHVLPDGPLADVFGDPSRHNAVPVILGSNRDEPALFMAASPEHVN
                     TYLWIFRRLKDEAGYRRQVKYLGQAWKARGVDEFANAMAAAGNPHVYAYRFDWDEEPS
                     VLGFDLSVALGAAHALEIPFVFGSFSGGLGLGYLYHESEGRHALAESITSYWTEFAYN
                     GDPGTGRDGGEVRWLPWGRNGATSIVLDTLEDKGIYMTTERVTTDSVKTAIATDPDVP
                     DQRERCLLYYRAFRWGSQFDVDEFAGFGDGGCAAYDPAALGDF"
     gene            complement(983..2215)
                     /locus_tag="AC16QF_13990"
     CDS             complement(983..2215)
                     /locus_tag="AC16QF_13990"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6449720.1"
                     /translation="MARGPTTVSTTFQAGWRSPLMLLMLMSVAMPVAFNAWSALLNNF
                     VVERAAFTGVEIGILQSLREVPGFLAFTTIFVLLVVREQTFAVLSLAMLGLGVALTGF
                     LPSEYGLYFTTVVMSTGFHYFETLKQSLSLQWLHRDEAPGMLGRLLAVGSLTSLVTYG
                     VLWVLLELFRLDYVWNFLISGGVCVGLAVVMWLWFPHFETKTPQTKRLVLRRRYWLYY
                     ALTFFSGARRQIFVVFAAFLMVEKFGYSAAQVTLLFLINYAFNWAFAERIGRLIGRFG
                     ERAALTFEYIGLIGVFVGYAFVSDPYVAAGLYVVDHMFFALAIAMSTYFQKVADPADI
                     ASSAGVSFSINHIAAVVVPAALGLLWIHSNALVFLIGAGFAVCSLLLSQLIPERPAPG
                     NEIARGRLFPQPADSAGR"
     gene            2415..2780
                     /locus_tag="AC16QF_13995"
     CDS             2415..2780
                     /locus_tag="AC16QF_13995"
                     /inference="COORDINATES: protein motif:HMM:NF012610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR6449721.1"
                     /translation="MPDWKDNEHIYLQLKDLVLRQIMLGTLLEGEPVPSVRQVASQER
                     INPLTVSRAYQMLVDEGLLEKRRGVGMFVCEGAREQALKQERTRFLEEDWPEVVERIA
                     LLGLTRDELLNTAAEEGRQ"
     gene            2777..3631
                     /locus_tag="AC16QF_14000"
     CDS             2777..3631
                     /locus_tag="AC16QF_14000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6449722.1"
                     /translation="MSLITARGLAKSFGDVRALDGLDLTVEAGRIVGLIGPNGSGKTT
                     ALKAILGLSHLDAGTLDVLGMNPATSRKALMQRTAYIADVGILPRWMRVSQLLDFVAG
                     VHPGFRREVAEAHLESTDVRPDKRINGLSKGMNVQLHLALVLAIDAELLVLDEPTLGL
                     DIIYRQKFYDALLGDYFDGNRSILVTTHEVREIEHILTDVVFIHHGRSLLSLSMESIH
                     DEFAKVVAQPTQAGAVRSLGPIAERTTLSGAEFVFRGADRDALGSYGEVSTPSLPELF
                     IAIMGDNA"
     gene            3628..4578
                     /locus_tag="AC16QF_14005"
     CDS             3628..4578
                     /locus_tag="AC16QF_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449723.1"
                     /translation="MSTMVALIRREWQEHQVGLGWSPVAILAVLALITFMALATSTVG
                     GVNVSVEGSEGSHSFHEEVEISSLSALAQFMELDRWSDAELEENLAKFRSLVAMPFKA
                     VYFLIAVFVLISALHDDRRDRSVLFWKSMPVSDAETVLSKLVTVVWVAPVVVIAGIVA
                     AQLFLLIVISTLTLVEDVGGAGRLWWHSGLLTGTVELVVGYVIQGFWALPLYAWLLAV
                     SAAAPRLPILWAVLLPGLPAIAERMVLGTHGVSDFVRAHLEFAALPAAASEDDGIIQP
                     AVGLAEQLSLVFSFQMWLGVVVGGLLLWLAVYLRNRNNDL"
     gene            4652..5944
                     /locus_tag="AC16QF_14010"
     CDS             4652..5944
                     /locus_tag="AC16QF_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449724.1"
                     /translation="MAWDFSTEPEFQKELDWMDEFVREEIEPLDFIIGHPNDFSDPNR
                     AKLIPPLQEEVKKRGLWACHLGPELGGQGYGQVKLGLMNEILGRAKCAPVVFGCQAPD
                     SGNAEILAHYGTPEQKDRYLEPLLNNEICSCYSMTEPQGGADPKVFTCRAELIGDEWV
                     INGEKWFSSNARYASFLIVMAVTDPEAPPYQRMSMFIVPTETAGIEIVRHVAVGYESK
                     GAHAYIRYNDVRIPRDHMLGEPGQGFIVSQVRLGGGRIHHAMRTMGKARRAFDMMCER
                     ALSRETQGEVLAQKQLVQAMIADSWIDIEQFRLFVLRTAWKIDKLKNYKEVRADISAV
                     KAQMPKVYHDVYARALQVHGSLGLSTEMPFAAGVLDSFHMGLADGPTEVHKVTVARQV
                     LGQYQGTDDLFPEYHLIRRKEAALAKYADIIEHEVGNA"
     gene            complement(5961..6737)
                     /locus_tag="AC16QF_14015"
     CDS             complement(5961..6737)
                     /locus_tag="AC16QF_14015"
                     /inference="COORDINATES: protein motif:HMM:NF018777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HIR6449725.1"
                     /translation="MIRITFLLRRKPELSAEEFYRYWREEHGPLVASHASRLNTLRYV
                     QVHTIDDPANQAMATARGGMEAPYDGVAEMWWESRAALVASLETKGGRRAGAELVEDE
                     ARFIDLPNSPMWLGHEYPQVNPTPENLVARERDTLVKLYFPLRPVQGLDERDAQSYWY
                     SNHGPIIRRQAAGVGIQRYVQVHRVADELEAGLRKARGTQVEGYMGHAELWFDRTVLG
                     VSTPERRAANERAIADESNFIDFRRSCMWLAKEHVFVDRR"
     gene            complement(6837..8045)
                     /locus_tag="AC16QF_14020"
     CDS             complement(6837..8045)
                     /locus_tag="AC16QF_14020"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6449726.1"
                     /translation="MALGDRHYRWVVVGYTLVIQGVSIGMLVYSFALFAIPWLDLFAA
                     PRRDVMLTVAILQLVNGAVSPLAGRAMDRYSIRTIVLAGLALMLVGLVVVSRAQALWQ
                     VWLVYATLFPVSMAMMSTLASQTLVTRWFDSRRGVAIGISATGTNVGGIVFPLLIAGW
                     LTDFGWRETFLWLAVTAAVVVGPLSWFVLGRTPAGHHPATRTTPGGGSVWTTRAILST
                     RRFWIPGLAILPLNLAFGAVQYNLGVLSRDLGLATGTAANLIALHAVCMIAGKFFFGS
                     LGDRIDHRYLFWLSAGLMVAALLVLQGETPVWMLVTGIVMMGLAGGGILPMFGMVFGA
                     RFGVAAFGRVMGLVMTTMTIGAMGPLLAGWAYDLTGSYDAAFLGMALLLLPAAVAMIW
                     LPQPGTDTAS"
     gene            complement(8047..9222)
                     /locus_tag="AC16QF_14025"
     CDS             complement(8047..9222)
                     /locus_tag="AC16QF_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449727.1"
                     /translation="MSAFRDDVRGWLEENCPPSMRTPLPADEYPGGGRRAVYGNPDTG
                     VWMQRMAERGFTVPLWPTEYGGAGLDAGENQVLQEELRRINARPALLGMGISMIGPAL
                     LEFGTEAQKREHLPKIARGEIWWCQGYSEPNAGSDLAGLGTSAVEDGDDYIVNGQKIW
                     TSGADRADWIFCLVRTDPSAPKHEGITFILFDMASPGVTVKPIELISGYSPFCETFFD
                     NVRASRRNVVGEPNKGWTVAKRLLQYERTSIGGIGGSSQKAATLEEIAREALGTVDGR
                     IADADLRAGILRHRMNDRAFGLTMKRSVQQTAAGIAPGAESSFFKYYGTEQNKGRYEL
                     LMSLLGTRGLGWSGPGFSDEERDATRAWLRSKANSIEGGTSEVQLNVIAKRILGLPD"
     gene            9364..10674
                     /locus_tag="AC16QF_14030"
     CDS             9364..10674
                     /locus_tag="AC16QF_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012614627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR6449728.1"
                     /translation="MSESTDRPATDDTHQFGFHTRSVHAGSRPDPATGARALPIYQTS
                     SYVFEDSESAAAYFNLQEYGNTYSRIMNPTVAALEERIANLEGGAGAVAFASGLAAQS
                     AAFFTLLEPGDHVVASAALYGGTVTQLKHLFRKMSVALTFVDPDRIDTWEAALQPDTK
                     LLFAETIGNPGGNVLDIEPLAELAHRHGMPLMVDNTFATPYLTRPMEWGADIVVHSAT
                     KFLGGHGTSIGGLVVDSGEFDWSNGRFPVIAEPSPAYHGLAFHETFGTYGYLMKLRAE
                     TLRDLGAAMSPFNAFLFAQGLETLPLRMRQHVANAEAVAAYLDGREDVERVSYPGLPG
                     SPYRDRVARYLPRGAGAIFCFDLPGGREAGQRFIEQLTLFSHLANVGDAKSLVIHPAT
                     TTHRQLSDEELAAAGIGPGTIRVSIGIEDADDIIWDLEQGLAGI"
     gene            10676..11161
                     /locus_tag="AC16QF_14035"
     CDS             10676..11161
                     /locus_tag="AC16QF_14035"
                     /inference="COORDINATES: protein motif:HMM:NF024773.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR6449729.1"
                     /translation="MTEHDMERYQDPHVIQGALNQRRIAVVGLSGNPLRASNFVGYYM
                     KRHGYEVVPVNPREEEILGLKSYPDLAAVPEPVGVVDVFRDPSAVPDIARAAVAAGAK
                     YLWLQFGVISFEGAEIAEAGGMQVIMDRCIKIEHARYRGRMHWLGFNTNQISAQRRRA
                     D"
     gene            11292..11804
                     /locus_tag="AC16QF_14040"
     CDS             11292..11804
                     /locus_tag="AC16QF_14040"
                     /inference="COORDINATES: protein motif:HMM:NF016408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR6449730.1"
                     /translation="MFRKVKGGTRMAPAMGERDEIRGELTHFSPADAANEATIGATIV
                     IKGEITGDENLVIEGTLDGTANLSDRNLTIAQTGRILGNVSANSITVEGEIQGNLTAT
                     ERVFVAKTGKVLGDIMAPRVSVEGGAKFTGRIDMDTKGGRVVRPSGPKRSETLTRPAA
                     ETAPAAENKA"
     gene            complement(11868..13124)
                     /locus_tag="AC16QF_14045"
     CDS             complement(11868..13124)
                     /locus_tag="AC16QF_14045"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACS family MFS transporter"
                     /protein_id="HIR6449731.1"
                     /translation="MSALPRRHVVVGLAALSVFVCYIDRVNISVAIIPMTEDLGWSLA
                     TQGTVLSSFFVGYLLLQIVGGRLADRFGGKVVLGVGVLVWSLFTVLTPPAAYLGLGVL
                     IAARIGMGMGEAVTFPSIYSLYGRWVPLSERARAVGFTNSGIPLGTVFALVATPYIVE
                     WLGWEWAFYLFGAVGVFWFVAWHRFVTAAPQAHPGVCDDELQVIEAGTQVAQAGESPP
                     LRVFLKLPAVWAIVVAHFCNNWSLYVLLSWLPTFVNKGLGVDYSAVGWFTMIPHLTAF
                     VFLNVAGGVADRLIHRGMDVGRVRKLMQTIGFGGIAAALLVVGEVQSAWMAIGVMSVG
                     NALGAFCTGGFAVNHMDIAPRHAGTLMGITNTAGTLPGIVGVFVSGVILELTGSWALV
                     FQVAAGVNLFGLVFYLTFASGRRLID"
     gene            complement(13212..13853)
                     /locus_tag="AC16QF_14050"
     CDS             complement(13212..13853)
                     /locus_tag="AC16QF_14050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_1061 family FMN-dependent PPOX-type
                     flavoprotein"
                     /protein_id="HIR6449732.1"
                     /translation="MGDETFAAFEGVVSDEESLRQVLGPPMQLAVDKVIDHIDDVSRA
                     IIEKSPFALLASATADAYPDVSPKGDPAGFVRVIDENYIAIPDRPGNRRGDTLTNLLD
                     NPYLAVIFLIPGKGETLRVSGECRIVRDRALRESMAVKGRVPELAIVMHVERVFIHCP
                     KCVVRSSLWDPQAWPDTSDLPNVAEAMIAHTKVKITPDELVEIAEKEDVVRLY"
     gene            13971..15071
                     /locus_tag="AC16QF_14055"
     CDS             13971..15071
                     /locus_tag="AC16QF_14055"
                     /inference="COORDINATES: protein motif:HMM:NF025627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HIR6449733.1"
                     /translation="MSRSHLLRTGLIHHDRESAFAGYTLFCGGQPASAHLIDMQGRIC
                     RRWENDMGISYAQLLADGNLLCRASSLPEVGGVRGLNGQSPCVYELDWDGNLVWAFHD
                     DWLHHDQHRLANGNTLLLAWRRVPAEITARVAGGRRSADDPEEMLGDVILEVDPSGAI
                     VRQWNSWEHLDPEEDAICPIDHRLEWTHANSITTTPSGEWLISLRRTNTVMAIEPDSG
                     EIAWRWGPGTLAHQHDARILDNGNLLVFDNGAHRSGEAEYSRVLEVDVPSDEIVWRYQ
                     SNPPFHFYSFMAGGAQRLPNGNTFICDAAVGRLFEVSPVGEIVWEYVNPFYASNPRLG
                     GDINIVFKASRHGPDDPGLAAKHLDPNRYENA"
     gene            15255..16811
                     /locus_tag="AC16QF_14060"
     CDS             15255..16811
                     /locus_tag="AC16QF_14060"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HIR6449734.1"
                     /translation="MRLHDYLEFFARENPDHPFADMNGASISYAQANERANRMAHGYL
                     ASGLEKGDRISYLSKNSIDMAIMFFAASKAGVVPVPLNYRLAPREWLYIINDAQSRTL
                     FCSGEYVDGIDSVRDKLNQVNAFVCTTGTADGWTDNESWLSKETANPGLDISENDQLY
                     QMYTSGTTGHPKGAMLSHRAIDCNTRMISSVMTLPVGSARILIVAPMYHAAAGISIMN
                     GISNGATLVIHEDFHPGHVIQALSDGGITSATLVPAMIQACLVSVPDAGERAYPSLEY
                     MAYGASPIAEETLRQAMAVFKCNFAQVFGMTETTAMATVLGPAEHRRALAGEPALLKS
                     AGRAALGTEVKIVDEEDREVDRGTIGEVIVRGPQVMMGYWNLPEATEKSLKDGWMHTG
                     DAAYMDDEGFIYIQDRIKDMIVSGGENIYPTEIENALFEHDAVADAAVIGIPSEQWGE
                     SVLAFLVLKDGQTVTPEEMIEFCRPRLAGYKIPRRIEFLDAIPRNASGKILKKDLREP
                     FWEGVERRVS"
     gene            complement(16860..17585)
                     /locus_tag="AC16QF_14065"
     CDS             complement(16860..17585)
                     /locus_tag="AC16QF_14065"
                     /inference="COORDINATES: protein motif:HMM:NF018777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HIR6449735.1"
                     /translation="MSDEGRVPATDGVRMMFLIRRRVGSSREELVAHWFANHMPAVIA
                     AMQGTARYYMAVLYDADASGSYPWDGVAQILVDEAMPTPSEPFGTTPTDSFQEHAEPY
                     WPWATTEYVVIDGSENLPVRPLTLNDPFPTTRSGFFKISFLVKAKPGTDHGAFFDHWL
                     DVHVPNVAGTMDEVGGFRYVVSHSLNPERAPYAGMAELYFHDEDGWRRYKAAIQPDGM
                     ERWAADEGTLVLRSHTEMIGIPG"
     gene            17666..18178
                     /locus_tag="AC16QF_14070"
     CDS             17666..18178
                     /locus_tag="AC16QF_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449736.1"
                     /translation="MPALDDRSLGWLRYLHRKATTPDNWDRDGRPHPDRYRSIAPAGL
                     WGEYDVSGWTANGIEPYGMQPDPVAADGMLFYKGFFNLLMSLHRYVSGSTKWDQPFEM
                     IRDEANTFEWAHSGADLYYDPVADEHVGTGWSMQTPGRETVPLTGENNRVNVQLTTDN
                     RTVIVRRSVG"
     gene            18278..18598
                     /locus_tag="AC16QF_14075"
     CDS             18278..18598
                     /locus_tag="AC16QF_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449737.1"
                     /translation="MPLHIAKYERRDHTDRTDLALAALEACRSARATAGVNSSRFYWV
                     NPDEIAIVTDAEPGAWGQGSGNQPEPRAMKAMFELADLARNTSVETWADARSGEETFK
                     ISQS"
     gene            18631..19980
                     /locus_tag="AC16QF_14080"
     CDS             18631..19980
                     /locus_tag="AC16QF_14080"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6449738.1"
                     /translation="MNDIVKHPLAMDGKDPRNLRGDPITPERYYSRDYMQQEWDNLWT
                     KIWHIAGREKQLAEPGDFIVHDFMRESVIIVRQRDGSLRGFYNACGHRAQRLVWNHGH
                     QESFLCPYHGWQWGLDGSLEDLPDRDDYPQGDPVGKLGLIELRVDTWAGLVWYTMDDE
                     APDLMKYLEPLPEIYRSFQFEKTVRSRWLRVELECNWKFWSDNFNESYHTRTAHPQVA
                     PIIDQDHFTSRYEMFPMGHARIIQMGRPSLRDRVPEGQPHPWDKELRFWGIDPDGYPD
                     YETKVMQGWLDLKAAKRTRWKEKGFHHYEHLTDEELTESPFQTVFPNIAFGASADSFG
                     VFRWEPHPTDPEKCYFDIWSMTYPVEGIEETVHRTARNPLKFEEAEYEFRVYDNGQGV
                     LDLSDQVVFQDWMLNAGQTTGWHSRGYQEPYLAAQETRVRRFHEVLHDYLAGNPPGR"
     gene            20055..20432
                     /locus_tag="AC16QF_14085"
     CDS             20055..20432
                     /locus_tag="AC16QF_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449739.1"
                     /translation="MPRFVLIGLCEPPNPESQQQFDEWFVDQHIEDTAKCPNFIRGSV
                     YKLSGPHLDGETVSGYLSLYEVEAPSYPEAERVLNEWQANPEAWEGRKHHLATAQKLG
                     GMPLAIKGSGWYELLKSFDGPAA"
     gene            complement(20452..21243)
                     /locus_tag="AC16QF_14090"
     CDS             complement(20452..21243)
                     /locus_tag="AC16QF_14090"
                     /inference="COORDINATES: protein motif:HMM:NF021465.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HIR6449740.1"
                     /translation="MSRDPASEGRVSIRTLDQLALWSSLSIVVALSWWYLFLLAQDMA
                     DMAGMPMGDMAGMHPWTALDFLLMFLMWAVMMVGMMVPTAVRAIYIYARVASQAQARG
                     SPVAATQIFVAGYVLVWAAFSLGATALQAALDALGLLSPAMRSSSPLLGAGLLVAAGV
                     YQLTPWKDTCLRHCQSPAAYLAGRFGPRALDALSLGIRHGLYCLGCCWVLMLLLFLGG
                     VMNLLWIAAIAAFVLLEKVLPPALRVSRLAAVMMIASGAAYLMIA"
BASE COUNT         4007 a         7211 c         6911 g         3245 t
ORIGIN      
        1 ctgatcgccg acggccgcgc aagctccccg gaggacgcca ggcgggttca ggagaacatg
       61 ccggccggcg ccatccgcga ctatctttac ggcaagagcc ttaccgagct gttcgccgtg
      121 ttcgataacg ggttcgccgg gatgatcgac acgccggagg ccctcggcga cggccacgtg
      181 cttcccgatg gtccgctggc cgacgtgttc ggcgacccct cgcgccacaa cgccgtgccc
      241 gtgatcctcg gcagcaaccg ggacgagccc gccctgttca tggccgcgag ccctgagcac
      301 gtgaatacct atctgtggat cttccgcagg ctgaaggacg aggccggcta ccggcgccag
      361 gtgaaatatc tcggccaggc gtggaaggca cgcggcgtcg atgagttcgc caacgccatg
      421 gcggccgccg gcaaccccca cgtctacgcc taccgtttcg actgggacga ggagccaagc
      481 gtgttgggct tcgatctgag cgtcgccctc ggcgcggccc acgccctgga gatccccttc
      541 gtcttcggtt ccttctcggg cgggctgggg ctcggctacc tctaccacga aagcgaaggc
      601 cgccacgcgc ttgccgagag catcacttcc tactggacgg agttcgccta caacggcgac
      661 ccgggcacgg gacgggacgg cggtgaggtc cgctggttgc cctggggccg gaacggcgcc
      721 acgtccatcg tgctggacac cctcgaggat aagggcatct acatgaccac cgagcgcgtg
      781 accacagact cggtgaaaac cgccatcgcg acggacccgg acgtccccga tcagcgcgag
      841 cgctgcctgc tctactaccg ggctttccga tggggatcgc agttcgacgt cgatgagttc
      901 gccggattcg gcgacggcgg ctgcgccgcc tacgaccccg ctgccctggg cgacttctga
      961 ccgatctcca ggggaggcgg cgtcagcggc cggcggaatc ggccggctgc ggaaacagcc
     1021 ggccccgggc gatctcgttg ccgggggccg ggcgctcggg gatcagctgg gagagcagca
     1081 gcgagcagac ggcgaaaccg gcgccgatga gaaagaccag ggcgttggag tggatccaga
     1141 gcaggcccag agcggccggc accaccaccg cggcgatgtg gttgatggag aaggacaccc
     1201 cggcggagct ggcaatatcc gccggatcgg ccaccttctg gaagtaggtg ctcatggcga
     1261 tggccagcgc gaagaacatg tgatccacca cgtacagacc ggctgccacg tacgggtccg
     1321 acacgaaggc gtagcccacg aacacgccga tgaggccgat gtactcgaag gtgagcgccg
     1381 cccgctcgcc gaagcggccg atgagccggc cgatgcgctc ggcgaacgcc cagttgaagg
     1441 cgtagttgat caggaacagc agcgtcacct gggccgccga gtagccgaac ttctcgacca
     1501 tcagaaacgc cgcgaagacc acgaagatct gccgccgggc gccggagaag aacgtcagcg
     1561 cgtagtagag ccagtagcgg cgccgcagca ccaggcgctt cgtctggggc gttttcgtct
     1621 cgaagtgggg aaaccacagc cacatgacca cggccagccc cacgcagacg ccacccgaga
     1681 tcagaaagtt ccagacgtag tccagccgga acagctcgag caacacccat agcacgccgt
     1741 aggtcaccag cgaggtgagc gaccccacgg caagcagccg tcccagcatg ccgggcgcct
     1801 cgtcccggtg cagccactgc agggaaaggg actgcttgag cgtctcgaag taatgaaagc
     1861 cggtggacat gaccacggtg gtgaaataaa gaccgtactc cgaaggcaga aagccggtaa
     1921 gcgcgacgcc gagccccagc atggccagtg acagcacggc aaacgtctgc tcgcgcacca
     1981 cgaggagcac gaagatggtg gtgaacgcca ggaaacccgg cacctcgcgc aggctctgca
     2041 gaatgccgat ctccacgccg gtgaaggccg cacgctccac cacgaagttg ttgagcaggg
     2101 cagaccaggc gttgaacgcg acaggcatgg ccaccgacat cagcatcagc agcatcagcg
     2161 ggctgcgcca tcctgcctgg aacgtggtgg agacggttgt gggtcctcgg gccaatgcgg
     2221 tgcggtccag tgatatgccc ggctgggcga aggggcgatt ctaaaggagg cgccgccgga
     2281 actcgacgcc atgaacgggc ccagaaaccg ggccctgatg gccgcagggg cccgaataag
     2341 ccggggttgc ccccggaatc gttatggtgt tatggttatg tataacacta ctgcacatat
     2401 agagcgacgg ccccgtgccc gactggaagg acaacgagca catctatctt cagctgaagg
     2461 acctggtcct gcggcagatc atgctgggca cgctgctcga aggcgagccg gtcccctcgg
     2521 tgcgccaggt cgcaagccag gagcgcatca atcccctaac cgtctcccgc gcctaccaga
     2581 tgctggtgga cgagggcctt ttagaaaaac gccgcggcgt gggcatgttc gtctgcgagg
     2641 gtgccaggga gcaggccctg aaacaggaga gaacgcgttt tctcgaagag gattggcccg
     2701 aggtggtcga gcgcatcgcc ctgctgggcc tgacccggga cgagctcctg aacaccgctg
     2761 ccgaggaggg ccgccaatga gcctcatcac cgcccgcggt cttgccaaat ccttcggcga
     2821 cgtgcgcgcc ctggacggcc tcgacctcac cgtggaggcc gggcgcatcg tcggcctgat
     2881 cggccccaac ggctccggca agaccaccgc cttgaaagcc atcctcggcc tgtctcacct
     2941 cgacgccggc accctcgacg tactcggcat gaacccggcg accagccgga aagcgctgat
     3001 gcagcgaacg gcctacatcg ccgacgtggg catattgccc cgctggatgc gcgtctccca
     3061 gctgctggac ttcgtagctg gcgtgcatcc cggcttccgc cgcgaggtag cggaagccca
     3121 tctcgaatcc accgacgtcc gtccggacaa acgaataaac gggctgtcca agggcatgaa
     3181 cgtccagctg cacctggccc tggtgctggc catcgacgcc gagctgctcg tgctggacga
     3241 gccgaccctg gggctcgaca tcatctaccg gcagaaattc tacgacgcgc tgctgggcga
     3301 ctacttcgac ggcaatcgca gcattctggt caccacccac gaggtccgcg agatcgagca
     3361 catcctcacg gacgtggtgt tcattcacca cggcaggagc ctgttgagcc tctcgatgga
     3421 aagcatccac gacgagttcg ccaaggtggt cgctcaaccg acgcaagccg gcgcggtgag
     3481 gtccctcggg cccatcgcgg agcgcaccac cttgagcggt gccgagttcg tgtttcgcgg
     3541 cgccgacagg gacgccctcg gcagctacgg cgaggtcagc acgccgtctc ttcccgagct
     3601 cttcatcgcc atcatgggag ataacgcatg agcaccatgg ttgccctgat cagacgcgaa
     3661 tggcaggagc atcaggtggg cctcggctgg tccccggtgg ccatcctggc cgtgctcgcg
     3721 ctcatcacct tcatggccct ggcgacgtcg accgtgggcg gggtcaacgt ctccgtggag
     3781 ggcagcgaag gcagccacag ctttcacgag gaagtggaga tctcgagcct ctctgcgctc
     3841 gcgcagttca tggagctcga ccggtggtcg gacgccgagc tcgaggagaa cctcgccaag
     3901 ttccgcagcc tggtggcgat gccgttcaag gccgtgtact tcctgatcgc cgtgttcgtc
     3961 ctgatctccg ccctgcacga cgaccggcgc gaccgcagcg tcctgttctg gaaatccatg
     4021 ccggtgagcg atgccgagac ggtgctcagc aagctcgtca ccgtggtctg ggtcgccccc
     4081 gtcgtcgtca tcgcgggcat cgtggccgcg cagctcttcc ttctcatcgt catctccacc
     4141 ctgaccctgg tggaggacgt cggcggcgcg gggcggctgt ggtggcattc cggcctgctg
     4201 accggaaccg tggagctcgt ggtcggctac gtgattcagg gcttctgggc cctgcccctg
     4261 tacgcctggc tgctggccgt gtccgcggcg gcgccccggc tgccgatcct gtgggccgtg
     4321 ctcctgcccg gcttgccggc cattgccgag cgcatggtgc tcggcaccca tggggtaagc
     4381 gacttcgttc gcgcccatct ggagttcgca gcacttccgg cggccgccag cgaggacgac
     4441 ggaatcatcc agcccgccgt ggggctggcc gagcagctct cgctggtctt cagcttccag
     4501 atgtggctgg gggtcgtcgt gggcggcctg cttctctggc tcgccgtgta cttgcgcaac
     4561 cgcaacaacg acctctgaag acgacctcgg agaacgacct gcaaagacgc ctctggtaag
     4621 ctcgccttct tcaccaggga ggacgcacgc catggcatgg gatttctcga ccgagcccga
     4681 gtttcagaaa gagctggact ggatggacga gttcgtccgc gaggagatcg aaccgctcga
     4741 cttcatcatc ggccacccca acgacttctc cgaccccaac cgggccaagc tcattccgcc
     4801 cctgcaggaa gaggtgaaga agcgcggcct gtgggcctgc catctgggcc ccgagctcgg
     4861 cggccagggc tacggccagg tgaagctggg gctgatgaac gagatcctcg gccgggccaa
     4921 atgcgcaccc gtggtcttcg gctgccaggc gccggactcg ggcaacgcgg agatcctcgc
     4981 ccactacggc acgccggagc agaaggaccg ctacctggag ccgcttctga acaacgagat
     5041 ctgctcctgt tactccatga cggaacctca gggcggcgcc gatcccaagg tgttcacctg
     5101 ccgcgcggag ctcatcggtg acgagtgggt catcaacggg gagaagtggt tttcctccaa
     5161 cgcccgctac gcgtcgttcc tcatcgtgat ggcggtgacc gacccggagg caccccccta
     5221 tcagcgcatg tccatgttca tcgttcccac ggagacggcc ggcatcgaga tcgtccgcca
     5281 cgtggccgtg ggctacgagt ccaagggggc ccacgcctac atccgctaca acgacgtgcg
     5341 catcccccgg gaccacatgc tcggggagcc gggccaggga ttcatcgtct ctcaggtgcg
     5401 gctcggcggc ggccgcattc accacgccat gcgcaccatg ggcaaggcgc gccgcgcatt
     5461 cgacatgatg tgcgagcggg ccctgtcgcg ggaaacccag ggggaagtgc tggcgcagaa
     5521 gcagctcgtc caggccatga tcgcggactc ctggatcgac atcgagcagt tccgcctgtt
     5581 cgtgctgcgc accgcgtgga agatcgacaa gctcaagaac tacaaggaag tccgcgcgga
     5641 catctcagcc gtcaaggccc agatgcccaa ggtctaccac gacgtctatg cccgcgcgct
     5701 gcaggtgcac ggctccctgg gcctgtccac ggagatgccc tttgctgccg gcgtgctgga
     5761 ctcgttccac atggggctgg ccgacgggcc caccgaggtt cacaaggtga ccgtggccag
     5821 gcaggtgctc gggcagtacc agggtaccga cgacctgttc ccggaatatc acctgatcag
     5881 gcgcaaggaa gcggccctgg ccaagtacgc ggacatcatc gagcacgagg tgggcaacgc
     5941 ctgagccacc cgcgtcgccg tcaccggcgg tcgacgaaga cgtgctcctt ggccagccac
     6001 atgcacgagc gccggaagtc gatgaagttg ctctcgtcgg cgatggcgcg ctcgttggcc
     6061 gcccgccgct ccggcgtgga aacgccgagc acggtgcggt cgaaccacag ctcggcgtgg
     6121 cccatgtaac cctccacctg ggtacccctg gctttgcgca gcccggcctc cagctcgtcc
     6181 gccacccggt ggacctgaac gtagcgctgg atgccgacac cggcggcctg tcgccggatg
     6241 atgggcccgt ggttgctgta ccagtaactc tgggcgtcgc gctcgtccag gccctgaacc
     6301 ggtcgtagcg ggaagtacag cttcaccagg gtgtcgcgct cccgggcgac cagattctcc
     6361 ggcgtcgggt tcacctgggg gtactcgtgg ccgagccaca tgggcgagtt gggcagatcg
     6421 atgaaacgcg cctcgtcctc caccagctcg gcgcccgccc gccggccgcc tttggtttcc
     6481 agagaggcca cgagcgccgc acggctctcc caccacatct cggcaacgcc gtcgtacggc
     6541 gcttccatgc cgccccgggc ggtggccatg gcctgattgg cggggtcgtc gatggtgtgg
     6601 acctggacgt agcgcagggt gttcagccgg cttgcgtgac tcgccaccag cgggccgtgc
     6661 tcctcgcgcc agtaacgata gaactcctcg gccgacagct ccggcttcct gcgcagcaga
     6721 aaagtgatgc gaatcatgct tcccccaaga aaaatttcga cagcccctgg ccgctgaaat
     6781 taaaccgcgg gggatctcac atgcaaccgg caccggccga aatgcatgcg cacgggtcag
     6841 gacgccgtgt cggttccggg ttgcggcagc cagatcatgg cgaccgcagc gggaagcagc
     6901 agcagcgcca tgcccagaaa agccgcgtcg tagctgcccg tgaggtcgta tgcccatccc
     6961 gccagcagcg gacccatggc gccgatggtc atggtggtca tgacgagccc catcacacgg
     7021 ccgaaggcgg ccacgccgaa acgcgcgccg aacaccatgc cgaacatggg caggatcccg
     7081 ccaccggcca gacccatcat gacgatgccg gtgacaagca tccacacggg cgtctcgccc
     7141 tgcagcacca gcagggcggc gaccatgagc ccggcggaca gccagaacag ataacggtga
     7201 tcaatgcggt cgcccaggct gccgaagaaa aacttgccgg cgatcatgca gacggcgtgc
     7261 aaggcgatga ggttcgccgc cgtcccggtt gccagcccca gatcgcggct gagcaccccc
     7321 aggttgtact gcacggcccc gaaggccagg ttcagcggca ggatggcgag cccgggaatc
     7381 cagaagcgtc gggttgaaag aatcgcccgg gtggtccaga cgctgccacc gcccggggtc
     7441 gtgcgggtgg ccggatgatg tccggccggc gttcgcccga gcacgaacca gctcagggga
     7501 cccacgacca ccgcagcggt gaccgccagc cacaggaagg tctcccgcca gccgaagtcc
     7561 gtcagccagc cggcaatcag caacgggaaa acgatgccgc cgacgttggt gcccgtggcc
     7621 gatatgccga tggcgacccc gcggcgcgag tcgaaccagc gagtcaccag ggtctgggaa
     7681 gccagcgtgc tcatcatggc catggagacg gggaagagcg tcgcgtagac gagccagacc
     7741 tgccacaggg cctgagcccg ggaaaccacg acgagcccga cgagcatcag cgccaggccc
     7801 gccagcacga tggttcggat ggagtatcga tccatggcac ggcccgccag agggctgacc
     7861 gcgccgttga ccagctgcag gatcgccacg gtgagcataa cgtcgcgacg cggggcggcg
     7921 aagaggtcga gccagggaat ggcgaagagc gcaaagctgt agaccagcat cccgatgctg
     7981 accccctgta tcaccagggt gtagccgacg actacccagc ggtaatggcg gtcgcccagc
     8041 gccacgtcag tcgggcaggc cgaggattcg tttggcgatg acgttcagct gcacctccga
     8101 ggtgccgccc tcgatggagt tggccttcga ccggagccac gcgcgcgtgg cgtcgcgctc
     8161 ctcgtcgctg aacccgggac cgctccagcc caggccacgc gtgccgagca gggacatcag
     8221 cagctcgtag cggcccttgt tctgctcggt tccgtagtac ttgaagaagc tggactcggc
     8281 gccgggggcg ataccggccg ccgtctgctg caccgagcgc ttcatggtca ggccgaaggc
     8341 ccggtcgttc atgcgatggc gcaggatgcc agcccggagg tcggcgtcgg caatccgccc
     8401 gtccacggtg cccagcgcct cccgggcaat ctcctcgagg gtcgccgcct tctggctgga
     8461 gccgccgatc ccgccgattg aggtccgctc gtactgcaaa aggcgcttgg cgactgtcca
     8521 gcccttgttg ggctccccca ccacgttgcg ccgcgaggca cgcacgttgt cgaagaaggt
     8581 ctcgcagaac ggcgagtagc cgctgatcag ctcgatgggc ttcaccgtca cgcccggcga
     8641 agccatatcg aacaggatga aggtaatgcc ctcgtgcttg ggcgcgctcg gatcggtccg
     8701 gaccaggcag aagatccagt ccgcgcggtc ggctcccgag gtccagattt tctgcccgtt
     8761 gacgatgtag tcgtcgccgt cttccaccgc ggacgttccc aggccggcca ggtccgagcc
     8821 ggcgttgggc tcgctgtagc cctggcacca ccagatctcg ccccgggcga tcttcggcag
     8881 gtgctcgcgc ttctgggcct cggtgccgaa ctcgagcagc gcgggcccga tcatgctgat
     8941 gcccatgccc agcagcgcgg gccgcgcgtt gatgcggcgc agctcctcct gcagaacctg
     9001 gttctcgccg gcgtcgagcc ccgccccgcc gtactcggtg ggccacagcg gcacggtgaa
     9061 gccccgttcc gccatccgct gcatccacac gccggtgtcc ggattgccgt agacggcacg
     9121 ccggccgccg ccgggatact cgtccgccgg caggggtgtg cgcatgctcg gcgggcagtt
     9181 ctcctccagc cacccacgca catcgtctct gaacgcgctc atcgctttcc tcgtcgaccc
     9241 atgaattcca ggcgagcaga cccgtcagaa cgcacgctag gcgttgcgac gcggccggcc
     9301 cgcataggtt atcttacctg cacaggcagc aggcagcgcg agcccgcgct gcggggaggc
     9361 agcatgagcg aatccacgga ccggccggct acggacgaca cccaccagtt cgggttccac
     9421 acccggtcgg tgcatgcggg cagccggcct gatccggcca ccggcgcgcg ggcgctgccc
     9481 atctaccaga cgagcagcta cgtcttcgag gactccgagt cggcggcggc atatttcaac
     9541 ctgcaggaat acggcaacac ctactcacgg atcatgaacc ccaccgtggc cgccctggag
     9601 gagcgcatcg ccaacctgga gggaggcgcg ggcgcggtgg cctttgccag cgggctggcc
     9661 gcccagtcgg ccgccttctt caccctgctc gagcccggcg accacgtggt tgcgtccgcc
     9721 gctctgtacg gcggcacggt gactcagctc aagcacctgt tccgcaagat gagcgtggcg
     9781 ctcaccttcg tggaccccga ccgcatcgat acctgggagg cggccctgca acccgacacc
     9841 aagctgctgt ttgccgagac catcggcaac cccggcggca acgttctgga catcgagcct
     9901 ttagccgagc tcgcccaccg tcacggcatg ccgctgatgg tggacaacac gttcgccacg
     9961 ccctacctca cccgacccat ggagtggggc gccgacatcg tcgtgcactc ggccaccaag
    10021 ttcctcggcg gccacggcac gtccatcggc ggcctggtgg tggattcagg agagttcgac
    10081 tggtccaacg gccgctttcc ggtgatcgcg gagccctccc ccgcctatca cgggctggcc
    10141 ttccacgaga ccttcggcac ctacgggtac ctgatgaagc tgcgggccga gaccctgcgg
    10201 gatctgggcg ccgccatgag cccgttcaac gccttcctgt tcgctcaggg tctggaaacc
    10261 ctgccgctgc ggatgcgcca gcacgtcgcg aacgccgagg ccgtcgccgc ctacctggac
    10321 ggtcgcgagg acgtcgagcg ggttagctac cccggcctgc ccggaagccc ctatcgagac
    10381 cgggtcgccc gctacctgcc gcgcggcgcc ggagccatct tctgcttcga cctgccgggc
    10441 gggcgcgagg cgggccagcg gttcatcgag cagctcacct tgttctccca tctggcgaac
    10501 gtgggcgacg cgaagagcct ggtcatccac ccggccacga ccacccaccg ccagctgagc
    10561 gacgaggaac tggcggccgc cggcatcggt ccgggcacca tacgcgtttc catcggcatc
    10621 gaggatgccg acgacatcat ctgggatctc gagcagggac tggcgggcat ctgacatgac
    10681 ggagcacgac atggaacggt atcaggaccc ccacgtgatc cagggggcgc tcaaccagcg
    10741 caggattgcc gtggtggggc tgtcgggcaa ccctctgcgg gcgagcaact tcgtgggcta
    10801 ctacatgaaa cggcacggct acgaggtggt tcccgtcaat ccccgggagg aagagatcct
    10861 cgggctgaaa agctatccag atcttgcagc cgtgccggag cccgtgggcg tggtggacgt
    10921 cttccgcgac ccgagcgcgg tgccggacat tgcgcgcgcc gcggtggcgg ccggcgcgaa
    10981 gtacctgtgg ctgcagttcg gcgtgatcag cttcgagggc gccgagatcg ccgaagctgg
    11041 cggcatgcag gtgatcatgg accgctgcat caagatcgag cacgcacgct atcgggggcg
    11101 catgcactgg ctcggcttca ataccaacca gatctccgca cagcgccgcc gggcggactg
    11161 aaatccgcag cgccgacctg gatatagcta cttgtcaatt cggccggtgc tcagttaatc
    11221 tgcacggtga aaggagcccc aatcgaacaa aagaaagggg gcatggggaa ttgggacacg
    11281 aggccgaagg catgtttaga aaggtcaaag ggggcacccg catggcaccc gccatggggg
    11341 aacgggacga gattcgcgga gagctaacgc acttcagccc tgcagacgca gcaaacgagg
    11401 ccacgatcgg agcaacgatc gtgatcaaag gagagatcac gggcgacgag aacctggtca
    11461 tcgaggggac cctggacggt accgccaacc tctcggaccg caatctcacc atcgcccaga
    11521 cgggtcggat tctcggcaac gtcagcgcca actcgattac cgtcgagggg gaaatccagg
    11581 gcaacctgac cgccaccgag cgggtgttcg tcgccaagac cggtaaggtc ctcggcgaca
    11641 tcatggcgcc cagggtgagc gtcgagggtg gcgccaagtt caccggccgg atcgacatgg
    11701 acaccaaggg tggccgggtc gtcaggccgt cgggtccgaa gcgctcggag accctgaccc
    11761 gcccggccgc cgagaccgcc ccggccgccg aaaacaaggc ctgagaacgc cgcagccgcg
    11821 gatgcggggc acctcgcgac gaggtcgtcc cgcgctcgcg cgcctgatca atcgatcagg
    11881 cgcctgccgc tggcgaacgt gagataaaac acgaggccga acaggttgac cccggcggcc
    11941 acctgaaaca ccagggccca ggatccggtc agctcgagga tcacgccgct gacaaacacg
    12001 cccacgatgc ccggcaacgt tcccgccgtg ttggtgatcc ccatgagcgt acccgcatgg
    12061 cgcggcgcga tatccatgtg gttgacggca aagccgccgg tgcagaacgc gcccagcgcg
    12121 ttgccgacgg acatgacgcc gatggccatc catgcggact gcacctcgcc gacgacgagc
    12181 agcgcggccg ctatgccgcc aaagccgatg gtctgcatca gcttgcggac gcggcccacg
    12241 tccattccgc gatggatgag ccggtcggca acgccgccgg ccacgttgag gaagacgaag
    12301 gccgtcaggt gcggaatcat cgtgaaccat cccaccgcgg agtagtccac gcccaggccc
    12361 ttgttgacga acgtcggcaa ccagctcagc agcacgtaga gtgaccagtt gttgcagaag
    12421 tgggcgacca cgatggccca gacggcgggc agcttgagaa agacccgaag cggcggcgac
    12481 tcgcccgcct gcgcaacctg agtacccgcc tcgatgacct gcagctcgtc atcgcagacg
    12541 ccagggtgcg cctggggcgc tgcggtcacg aagcggtgcc aggccacgaa ccagaacacg
    12601 cccaccgcgc caaagaggta aaaggcccac tcccagccga gccactcgac gatgtacggc
    12661 gtcgcaacaa gcgcaaaaac ggtgcccagc gggatgccgc tgttggtgaa cccgacagct
    12721 cgggcgcgct cgctcaacgg cacccagcgg ccgtagaggc tgtagatcga gggaaacgtc
    12781 accgcttcgc ccattcccat accgatgcgg gccgcgatca gcaccccgag gccgaggtag
    12841 gcggcgggcg gtgtcagtac cgtgaacagc gaccagacga ggacgccaac gccaagcacc
    12901 accttgccgc cgaagcggtc ggcgagccgg ccaccgacga tctgcagcag caggtatccg
    12961 acgaagaagg acgacagcac agtgccctga gtggccaggc tccagccgag atcctcggtc
    13021 atggggatga tcgccacgga gatgttgacc cggtcgatgt agcagacgaa caccgacaga
    13081 gccgccagcc ccaccaccac gtgccgacgg ggaagcgcgc tcatcgtgcg ctgctcggtc
    13141 ccggacgggg ccgcgccgcg tcaaccaggc gcggggccgt cacgacgtcc ggggagggaa
    13201 tcgccacggg ctcagtagag cctgaccacg tcctctttct ccgcgatctc gacgagctcg
    13261 tcgggagtga tcttcacttt ggtgtgggca atcatggcct cggcgacgtt gggcagatcc
    13321 gaggtgtccg gccaggcctg agggtcccac aaacttgacc gcaccacgca tttgggacag
    13381 tgaatgaaaa ccctctccac atgcatgacg atggcgagtt cgggcacacg acctttcacg
    13441 gccatgcttt cccggagcgc ccgatcgcgc acgatccggc attcgccgct gacccgcaac
    13501 gtctcgccct tgcccgggat cagaaagatc accgccagat aggggttgtc gagcaggttg
    13561 gtgagcgtgt cgccgcggcg gttaccgggc cggtccggga tggcgatgta attctcgtcg
    13621 atgacgcgca cgaacccggc cggatcgccc ttgggagaga cgtccggata cgcgtcggcg
    13681 gtcgccgatg ccagcagggc aaacggggat ttctcgatga ttgcccggct gacgtcgtcg
    13741 atgtgatcga tgaccttgtc caccgccagc tgcatgggcg ggccgagcac ctgccgaagt
    13801 gactcctcgt cggagacgac gccctcgaac gctgcaaacg tctcgtcgcc catgatcccc
    13861 tcctatctcg tggcgcgatc gtctcccgat catggtccat accccgcaac ggaatcaagg
    13921 cgcccctccc gacgtgacgc tcgcgccccg aagccgatac catcacgacc atgagccgat
    13981 cccacctgct tcgcaccggc ctgattcacc acgaccggga atcggcgttt gccggttaca
    14041 ccctgttctg cggcgggcag cctgcgagcg cgcatctgat cgacatgcag ggcaggatct
    14101 gccgccgctg ggaaaacgac atgggcatct cttatgccca gctgctggcc gatggcaacc
    14161 tgctgtgccg ggcttcctcg ctgcccgagg tgggcggcgt tcgcgggctg aacggccaga
    14221 gcccgtgcgt ctacgagctc gactgggacg gcaacctggt ctgggccttt cacgacgact
    14281 ggctgcacca cgaccagcac cgtctggcca acggcaacac cctgctgctc gcctggcgcc
    14341 gcgtgccagc ggagatcacc gcccgggtcg ccggcggccg gcgcagcgcg gacgaccccg
    14401 aggagatgct cggggacgtg atcctcgagg tcgacccgag cggcgcgatc gtcaggcagt
    14461 ggaactcgtg ggagcacctc gaccccgagg aggacgcgat ctgccccatc gaccatcgcc
    14521 tggaatggac ccacgccaac tccatcacca ccaccccgtc cggggagtgg ctgatcagcc
    14581 tgcgccgcac gaacacggtg atggccatcg agccagattc cggcgagatc gcctggcgct
    14641 ggggcccggg caccctggcc caccagcacg acgcccggat tctcgataac ggcaacctgc
    14701 tcgtgttcga caacggcgcg caccggtcgg gggaggccga gtactcgaga gtgctcgagg
    14761 tggacgtgcc gagcgacgag atcgtctggc gctaccagtc caacccgccc tttcacttct
    14821 acagcttcat ggccggcggc gcccagcggt tacccaacgg aaacacgttc atctgcgatg
    14881 cggcagtggg caggctcttc gaggtctcgc ccgtcggcga aatcgtctgg gagtacgtca
    14941 acccgttcta tgccagcaat cccaggctcg gcggcgacat caacatcgtc ttcaaggcga
    15001 gccgccacgg gcccgacgac ccgggcctcg cagcaaagca cctggacccc aatcggtacg
    15061 agaacgcctg aaaggcgcgg gatgcacgtt cgtccggcgc gccggagccg cgagcccttc
    15121 aggcctgcgc ggtcagcggg cccgccccgc gcgcggacgt tgccgagact atgcctgctg
    15181 agataagttg gctgggccga caacgcttcg gctcgatcgg cggacatcaa ttcgcaggca
    15241 tggggagggc cttgatgcgg ctgcacgact atctggaatt cttcgcccgg gaaaatcccg
    15301 atcacccctt cgccgacatg aacggcgcgt cgatctcgta cgctcaggcg aatgaacgcg
    15361 ccaaccgcat ggcgcacggc tacctggcat ccggtctcga gaaaggggac aggatctcgt
    15421 acctgtcgaa gaactcaatc gacatggcca tcatgttctt cgccgcctcc aaggccggcg
    15481 tcgtgcccgt gcccctgaac taccggctgg cgccccggga gtggctgtac atcatcaacg
    15541 atgcccagag ccggaccctg ttctgctccg gcgagtacgt ggacggcatc gacagcgtcc
    15601 gcgataagct gaaccaggtg aacgccttcg tgtgcaccac gggcaccgcc gacgggtgga
    15661 cggataacga gtcgtggctt tccaaggaaa ccgccaatcc gggcctagac atctcggaga
    15721 acgaccagct ctaccagatg tacacgtccg gcaccacggg ccaccccaag ggagccatgc
    15781 tcagccaccg cgccatcgac tgcaacacgc ggatgatatc gagcgtgatg acgctgcccg
    15841 tgggctccgc ccggatcctg atcgtcgcgc ccatgtacca cgcggcagcg ggcatctcga
    15901 tcatgaacgg catcagcaac ggcgcgaccc tcgtcatcca cgaggacttc catccgggcc
    15961 acgtgatcca ggcgctcagc gacggcggca taacgtcggc caccctggtg ccggccatga
    16021 tccaggcctg cctggtgagc gtgccggacg caggtgagcg tgcctacccc tcgctcgagt
    16081 acatggccta cggcgcgagc cccattgcgg aagagacgct gcgccaggcc atggcggtat
    16141 tcaagtgcaa cttcgcccag gtcttcggga tgacggagac cacggccatg gccacggtgc
    16201 tgggaccggc cgagcacagg cgcgcgctgg ccggcgagcc tgcgctcctg aaatcggcgg
    16261 gccgtgcggc cctgggcacc gaggtgaaga tcgtcgacga ggaggaccgg gaggtcgacc
    16321 gcggcaccat cggcgaggtg atcgtccgcg gaccccaggt gatgatgggc tactggaacc
    16381 tccccgaggc gacggagaag tcgctgaagg acggctggat gcacaccggg gacgcggcct
    16441 acatggacga cgagggtttc atctacatcc aggaccgcat caaggacatg atcgtctcgg
    16501 gcggcgagaa catctacccc acggagatcg agaacgcgct cttcgagcac gacgccgtag
    16561 ccgacgcggc cgtgatcggc atccccagcg agcagtgggg agagagcgtg ctggccttcc
    16621 tggtgctaaa ggacggccag acggtgaccc cggaggagat gatcgagttc tgccggccgc
    16681 gcctggccgg ctacaagatc ccgcgacgca tcgagtttct cgacgcgata cccagaaacg
    16741 ccagcggcaa gatcctcaag aaggatctcc gcgagccgtt ctgggaaggc gtagagcgcc
    16801 gggtgtcgta gaggggcctc cagctgggcg cccggtagcc tgccgctcag cccggccggc
    16861 tacccgggga tgccgatcat ctcggtatgg gagcgaagca ccagggtgcc ttcgtcggcc
    16921 gcccaccgct ccatgccgtc gggctggatg gccgccttgt agcgccgcca gccgtcctcg
    16981 tcgtgaaagt agagctcggc catgccggca tagggcgccc gttcggggtt gaggctgtgg
    17041 ctgaccacgt agcggaaccc accgacctcg tccatcgtcc ccgccacgtt gggcacgtga
    17101 acgtcgagcc agtgatcgaa gaacgcgccg tggtccgtac cgggtttcgc tttgaccaga
    17161 aacgagatct tgaagaagcc cgagcgcgtg gtcgggaacg ggtcgttcag ggtcaggggc
    17221 ctcaccggca ggttctctga gccgtcgatg accacgtact ccgtggtcgc ccagggccaa
    17281 tagggctccg cgtgctcctg aaaactgtcc gtgggcgtgg tgccgaaggg ctcggagggt
    17341 gtcggcatgg cttcgtccac gagaatctga gccacgccgt cccaggggta ggaccccgag
    17401 gcatcggcgt cgtaaagcac ggccatgtag taccgggcgg ttccctgcat ggcggcgatg
    17461 acggcgggca tgtgattggc aaaccagtgg gcgaccagtt cctcgcggct cgacccgacc
    17521 cggcgcctga tcagaaacat catgcgcacc ccgtccgttg cgggaacccg gccttcgtcc
    17581 gacatgagca tcctccccac ccgtcgcttg gctgcagcgt agcaccattg ctgccagctt
    17641 acccgcattg gaccgggagc tgccgatgcc tgcactggac gaccgctcgt taggctggct
    17701 gcgctatctg caccgcaagg ccaccacacc cgacaactgg gatcgcgacg gccgacccca
    17761 cccggaccgt taccgttcca tcgccccggc cgggttgtgg ggggagtacg acgtgtcggg
    17821 ctggacggcc aacggcatcg agccctacgg catgcaaccg gatcccgtgg ccgcagacgg
    17881 catgctcttc tacaaaggct ttttcaacct gctgatgagc ctgcaccgct acgtgtccgg
    17941 cagcacgaag tgggatcagc cgttcgagat gatccgcgac gaggccaaca ccttcgagtg
    18001 ggcgcactcc ggcgccgacc tctactacga cccggtggcc gacgagcacg tggggaccgg
    18061 ctggagcatg caaaccccag gccgagagac cgtgcccctg acgggcgaaa acaatcgcgt
    18121 gaacgttcag ctgacgaccg acaaccgcac ggtgatcgtg cgccggtctg tcggctgagg
    18181 tccgacctgg ccctgccggg cgttcgcccc ggggttccaa accgccttca ttcgggccat
    18241 tatccggaga cgggacagaa aggatcaggg gaaaaccatg ccattacaca ttgcaaagta
    18301 cgaacgccga gaccataccg accgtaccga cctggcactt gccgcgctgg aagcctgccg
    18361 cagcgcccgg gccacggcgg gggtcaacag cagccgcttt tactgggtaa acccggacga
    18421 gatcgccatc gtcaccgacg ccgaaccggg cgcctggggc cagggttccg gcaaccagcc
    18481 cgagccgaga gcgatgaagg ccatgttcga gctcgccgat ctggcgcgca acacgtcggt
    18541 ggaaacctgg gcagacgccc gctccggcga ggagacgttc aaaatctcac aatcctgatc
    18601 acacccggag ccaaaacgag gggatatccg atgaacgaca tcgtcaagca tccacttgcc
    18661 atggacggaa aagatccgcg caacctgcgc ggcgatccca ttacgccgga acgctactac
    18721 tcccgcgatt acatgcagca ggaatgggac aacctgtgga cgaaaatctg gcacatcgcc
    18781 ggacgcgaga agcagctggc ggagcccggg gacttcatcg ttcacgactt catgcgcgag
    18841 tcggtgatca tcgttcgcca gcgggacggc tccctgcgcg ggttctacaa cgcctgcgga
    18901 caccgcgccc agcggctcgt gtggaaccat ggccaccagg agagtttctt gtgcccctat
    18961 cacggctggc agtgggggct tgacgggtct ctggaggacc tgcccgaccg ggacgactat
    19021 ccccagggag accccgtggg caaactgggc ctgatcgagc tgcgcgtgga tacctgggcc
    19081 gggctcgtct ggtacaccat ggacgacgaa gcgccggacc tgatgaagta tctcgagccc
    19141 cttccggaga tctaccgcag cttccagttc gagaagacgg tacgctcccg ctggctgcgg
    19201 gtagagctcg agtgcaactg gaaattctgg tcggacaact tcaacgagtc ctaccacacc
    19261 cgcaccgctc atccccaggt agcacccatc atcgaccagg atcacttcac ctcgcgctac
    19321 gagatgtttc ccatggggca cgcgcgcatc atccagatgg gccgcccgtc gcttcgcgac
    19381 cgggttcccg agggccagcc gcacccgtgg gacaaggagc tgcgcttctg gggcatcgac
    19441 cccgacggct atcccgacta cgagaccaag gtcatgcagg gatggctcga tctcaaagcc
    19501 gccaagcgca cgcgctggaa ggagaaaggc tttcaccact acgagcacct gaccgacgag
    19561 gaactgacgg agagcccctt ccagacggtc tttcccaaca tcgccttcgg cgcctcggcc
    19621 gattcgttcg gcgtcttccg ctgggagccg cacccgaccg acccggaaaa gtgctacttc
    19681 gacatctgga gcatgaccta cccggtggag ggcatcgagg agaccgtgca ccgcacggcg
    19741 cgcaacccgc tcaagttcga ggaggccgag tacgaattcc gcgtctacga caacggccag
    19801 ggcgttctcg acctctccga ccaggtggtc ttccaggact ggatgctcaa cgccggccag
    19861 accacgggct ggcattccag gggctatcag gaaccgtatc tggcggccca ggaaaccagg
    19921 gtccggcggt tccacgaggt gctgcacgac tacctggcgg ggaacccgcc ggggcgctga
    19981 gccgaggggt cacggcgctc tccacacgag ggcgctccgt cccccttacg tatttgacaa
    20041 gtcaggacac acaaatgccg agattcgttc tcatcgggct ctgcgagccg cccaaccccg
    20101 aatcccaaca gcagttcgac gagtggttcg tcgaccagca catcgaggat accgccaagt
    20161 gccccaactt catccggggc agcgtctaca agctgagcgg gcctcacctg gacggcgaga
    20221 ccgtttccgg atacctctcg ctctacgagg tggaggcccc cagctacccg gaagccgagc
    20281 gggtgctcaa cgagtggcag gcaaatccgg aggcctggga gggacgcaag caccacctgg
    20341 ccaccgccca gaagctcggg ggcatgccgc tggccatcaa aggctcgggc tggtacgagc
    20401 tgctgaagtc gttcgacggg cccgccgcct gaacgttagc gactcgagcg gctaggcgat
    20461 catcaggtag gcggcgccgc ttgcgatcat catcacggca gcaagccggg atacacgcag
    20521 cgcgggaggc aggactttct ccagcagcac gaacgccgcg atggccgcga tccacagcag
    20581 gttcatcacg ccgccgagaa agagcagcag catcagcacc cagcagcagc caaggcagta
    20641 aaggccgtgc cggataccca ggctcagagc gtccagcgct cgcggcccga agcgtccggc
    20701 cagataagcg gccggggact ggcagtggcg caggcaggtg tccttccacg gggtgagctg
    20761 gtacacgccc gcggcaacga gcaacccggc gcccagcagc gggctggacg agcgcatggc
    20821 cggtgacagc aggcccaatg cgtccagcgc ggcctgcaac gccgtcgcgc cgaggctgaa
    20881 agcggcccag accagcacgt agccggccac gaagatctgg gtggcggcca ccggcgagcc
    20941 ccgggcttgg gcctggctcg caacccgcgc gtagatgtag atggcgcgca ccgcagtggg
    21001 caccatcatg cccaccatca tcacggccca catcaagaac atgagcagga agtccagcgc
    21061 ggtccaagga tgcatgcccg ccatgtcgcc catgggcatt ccagccatgt cggccatgtc
    21121 ttgggccagc aggaacaggt accaccagga cagcgctacc acgatgctca gcgacgacca
    21181 gagggcaagc tgatcgaggg tccggatcga gacgcgccct tccgacgccg gatctcggct
    21241 cacgcgggaa ttacgagccc gtcgtgatcg tagttgtgct cacagaactg gccgtagctg
    21301 ccctgcagcg acatgggcag ctcgccatgc acctccgcga atccctggcc gatctccgca
    21361 taggtgaact cgaa
//
LOCUS       DBNHLM010000304         2071 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_344497, whole genome shotgun sequence.
ACCESSION   DBNHLM010000304 DBNHLM010000000
VERSION     DBNHLM010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_344497"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="AC16QF_14095"
     CDS             complement(<1..212)
                     /locus_tag="AC16QF_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449741.1"
                     /translation="MRTVRHRARSTKDGRRVGLAFAGLLATAFFASCASYSAGSLNQI
                     HSELVWERRIECRKAWSAYESACRGNL"
     gene            complement(407..1420)
                     /locus_tag="AC16QF_14100"
     CDS             complement(407..1420)
                     /locus_tag="AC16QF_14100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019821071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR6449742.1"
                     /translation="MSLPFSNLARPLARQPSSACRASTRPTIITATTAIYPERKSEGG
                     RRRRPSGDGRLKWHQGANASILTPMEPSEATDTLIRLACFIGALVLFALLEAAFPRRQ
                     RQVSRKARWPSNLGISALNQLVLRLVLPASAIALAVRLEQEGWGLLAQLTWPTWIELA
                     AAVLILDLAIYLQHRLYHAVPFLWRFHRMHHADLDFDVTTGIRFHPLSLLLSGLIKLG
                     AVLIIGPSPFAVLVFEVLLNATTMFNHSNLKLPRPFDRILRLVIVTPDMHRVHHSADP
                     AETNRNFGFNFPWWDRLFKTYRPQPALGHEAMTVGLEDFRSAGELRLDRMLSQPFRSG
                     TGS"
     gene            1419..>2071
                     /locus_tag="AC16QF_14105"
     CDS             1419..>2071
                     /locus_tag="AC16QF_14105"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6449743.1"
                     /translation="MVTSRQEALVQRSQAVSGSVSRIFPADVTITGERYTSREFMELE
                     LDRLWPRVWNIGAWAREIPVAGDFVTHAIGRESILMVRQADGSVRAFHNVCPHRGNRL
                     VYSERGSVERFTCSYHGWQFDQAGTVVHVQDEEDFPRGNPCGKLTLAEIPCEVWAGFV
                     WYNMDAECASLAEFLGEVKDYVDLHDLSAARRIYYKVCEVGFNWKLLHDNFCESYHLP
                     "
BASE COUNT          413 a          602 c          699 g          357 t
ORIGIN      
        1 agattgccgc ggcaggccga ctcgtaggcg gaccatgcct ttctgcactc gatccgccgc
       61 tcccagacga gttcgctgtg aatctggttg aggcttccgg cggagtagga ggcacagctg
      121 gcgaagaagg cggtggcgag cagtcccgcg aaggccagac caacccgtcg accatccttg
      181 gtgctccgag ctctgtgtct gactgtccgc atctgctacc tcgctttgga ggaggacagc
      241 ccggcgaccc ctatcagcga tgaagatcta ccgggtcgat tcagatgtta ggagccaatg
      301 atggagagag actgcagaga ataacgagcg agccaacgca aatgccaaac gcgtcacaaa
      361 ccgggaaact ttccgaccgt tcaggcgtgt gcaagcctga attccgtcag ctgccggtac
      421 cgctccggaa gggttgagaa agcatgcggt cgagccggag ttcgcccgct gatctgaaat
      481 cctccaggcc caccgtcatg gcttcgtggc cgagcgcggg ctgaggccgg taggttttaa
      541 agaggcgatc ccaccacggg aagttgaagc cgaaattccg attcgtctcg gccggatcag
      601 cggaatggtg cacgcggtgc atgtcggggg tcacgatgac gagtcgcaga atccgatcga
      661 acgggcgggg aagcttcagg ttgctgtgat tgaacatggt ggttgcgttc agcagcacct
      721 cgaagacgag cacggcgaag ggtgacggtc cgatgatcag aaccgcgccg agcttgatga
      781 ggccggagag caacagtgag agcggatgaa accggatgcc cgtggtgacg tcgaagtcga
      841 ggtcggcatg gtgcatgcgg tgaaagcgcc ataggaaagg gaccgcgtga tagagccgat
      901 gctgcaggta gatggccaga tccaggatca gaaccgccgc cgccagctcg atccaggtcg
      961 gccatgtcag ctgagccaag aggccccagc cctcctgctc caggcggacg gcgagcgcga
     1021 tcgcagaggc gggcagcacc agccgcagga ccagctggtt cagagcggag atgccaaggt
     1081 tgctgggcca gcgtgctttg cgggacacct ggcgctgccg ccggggaaac gctgcctcca
     1141 gcagggcaaa caaaaccagc gcgccgataa aacatgccag gcggatgagc gtatcggtag
     1201 cttcgctcgg ttccatgggt gtaagaatgc tcgcattggc cccctggtgc cacttaaggc
     1261 gtccgtcgcc tgacggccgg cgccgacggc cgccctcgct tttgcgctct ggataaatcg
     1321 ctgtggtggc tgtaataatc gttggacgcg ttgacgcacg gcaagcggaa gagggttgtc
     1381 gtgcaagcgg ccgcgccagg ttggagaagg ggagcgacat ggttacgtcc agacaggaag
     1441 cgctcgttca gcgcagccaa gccgtatcgg ggagcgtgag ccggatcttt cccgctgacg
     1501 ttaccatcac cggcgagcga tacacctccc gcgagttcat ggagctggag ctcgaccgct
     1561 tgtggccccg ggtctggaac atcggggcct gggcgcggga gatcccggtg gcgggtgact
     1621 tcgtcaccca cgcgatcggc cgggagtcga tactcatggt gcggcaggcg gacggctcgg
     1681 tccgtgcctt ccacaacgtc tgcccccacc gcggcaaccg gctggtctac tcggagcggg
     1741 gatccgtaga gcgcttcacc tgcagctatc acggctggca gttcgaccag gccgggacgg
     1801 tcgttcacgt gcaggacgag gaggactttc cccgggggaa cccgtgcggg aagctcacgc
     1861 tggcagagat cccctgcgag gtctgggccg ggttcgtctg gtacaacatg gacgccgaat
     1921 gcgcctcgct cgcagagttt ctcggcgagg taaaggacta cgtcgacctg cacgatcttt
     1981 cggctgccag gcgcatctac tacaaggtct gcgaggtcgg cttcaactgg aagctgctgc
     2041 acgacaactt ctgcgagtcg taccatctgc c
//
LOCUS       DBNHLM010000305        11938 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_524618, whole genome shotgun sequence.
ACCESSION   DBNHLM010000305 DBNHLM010000000
VERSION     DBNHLM010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11938
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_524618"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="AC16QF_14110"
     CDS             complement(<1..1008)
                     /locus_tag="AC16QF_14110"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6449744.1"
                     /translation="MKIEADAALLTLALVAILVALAPAPALGEESARPIEEVVVTARY
                     REETAQESPTSVTAFNSVMLEQITAQDLRDVGPASPNVHIQPVVTFPNSAAIHIRGMG
                     QQNIESTNEMRTGVSINGVFISRPIATLIDFFDVETVEVLRGPQGTTFGKNSLAGGLA
                     VTTKRPDGSFDYAAELTGGNEGRLDFRGAIQFPIIEDKLSARISLLMQNYDGHFKNRV
                     NGEDLNGEDIDTIRGTIVWTPTDTFEATLIGTWLEERSDAPGGDDRSDPCPTQILSCI
                     FTEPDDGAFTVGRDALDFHNTDQNSITGIINWDIGSFTLTSVTGWMETDGFIASDFDQ
                     TE"
     gene            complement(1092..1631)
                     /locus_tag="AC16QF_14115"
     CDS             complement(1092..1631)
                     /locus_tag="AC16QF_14115"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6449745.1"
                     /translation="MQTNEKFEIRGINHIALVCRDMKETVDFYSGVLGMPLTKTIELP
                     NGMGQHFFFDIGKGDSLAFFWFPNAGDSQPGVTHAPDLVGRGDITSAHASMNHLAFDV
                     PEEKIEEYQEKLAAAGIDVTAVVNHDDSETQASPTVTESTFVRSIYFKDPDGILLEFA
                     AWTRELDERDVNCEPVSVA"
     gene            1761..2417
                     /locus_tag="AC16QF_14120"
     CDS             1761..2417
                     /locus_tag="AC16QF_14120"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6449746.1"
                     /translation="MPRLKQVSRAEATENVRKYYDALFGERDPVAEPGTATGTPGNWW
                     TVFALVPYVFDHATSHFGMFGMFADKSISQLDGKVRELGIMRAGYTQGSQFVFSQHCK
                     AARRFGISDEQIEAIPHWEVSDAFDAKERAVLAWADALILQGGRASDALFEALHSHLS
                     DEDILELTYHIMGYNLHAVCCKALRLEYDDVPERIREVPVPAEGETADWAGGAWQREE
                     "
     gene            2422..3009
                     /locus_tag="AC16QF_14125"
     CDS             2422..3009
                     /locus_tag="AC16QF_14125"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6449747.1"
                     /translation="MSDSDRIHYGGLHHLALVCRDMERTVDFYTNVLDMKLKKGFDLD
                     GGYGQHFFFDMGGGNELAFFWFRDAKDAQPGVASAAHLVGSGEGSITSAHGSMNHVAF
                     DVAPDQIEAYREKLVSRGVNCTPLVNHDDVVTGEESRRGSGISAKTWLRSFYFYDPDG
                     IMLEFCATLNTGSPEVELPVNAAGIKANGEPITGA"
     gene            3006..3482
                     /locus_tag="AC16QF_14130"
     CDS             3006..3482
                     /locus_tag="AC16QF_14130"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6449748.1"
                     /translation="MTNRDQTSTASPLEHASGPALLRELVHATYWLDDGLQAYMKEHA
                     GLSLPRAQSMMMVYIAEGVDSPSDLARRLRVSKQAIRQGLKELMAKDMVTLEPDPGNR
                     RQKVVRFTKHGRALRQVAWEGLQNLERELGRRLGGDRLAALHDALFLDWGDVPGEP"
     gene            complement(3575..3889)
                     /locus_tag="AC16QF_14135"
     CDS             complement(3575..3889)
                     /locus_tag="AC16QF_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449749.1"
                     /translation="MSAVNIIRIVALVLAVVGAFVTIPYAAAALVILGLVAGFMGVDE
                     ERRLMVLLMAVALAVVADGLGPIPAVGDYLTAILTNLSGVFDGLAIAVILTAIYERIT
                     QG"
     gene            4181..4627
                     /locus_tag="AC16QF_14140"
     CDS             4181..4627
                     /locus_tag="AC16QF_14140"
                     /inference="COORDINATES: protein motif:HMM:NF025473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABA4-like family protein"
                     /protein_id="HIR6449750.1"
                     /translation="MLDTIFSATNALAMIGWAGLILLPGRRFVVQILARVLIPAIIAV
                     IYIGLMVGHVGSFPEGGGFGSLAGIATLFSVDALLLAGWIHYLAFDLFVGSWEVTDSR
                     KEGIPHLLLVPCLVLTFMAGPAGLALYFAIKYGRRSLASEAAQPSQ"
     gene            4624..5469
                     /locus_tag="AC16QF_14145"
     CDS             4624..5469
                     /locus_tag="AC16QF_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449751.1"
                     /translation="MSERGRQPVLAGESPVEATPLLMGIRARHALWFDCAMGFAALAV
                     VCLLMLAMDERALNGVNVWAKPLKFSISLAVYFATLAWFAPLMPDGYFAGTGGRWLSA
                     IPVACAVFEIVYIIVQAALGEPSHFNVSTTFHSTMYTLMGGGAFILVAICLWMGLIIL
                     SVHRMSNPYALSVGLALVLTFVLGGGFGAYLGGQTGHWVGGTLSDADGLWLVNWSRDG
                     GDLRVPHFFGMHAMQILPALATLVPHRMTRDGANVAVLAMTLLYAAFAGWTFQQAVSG
                     IPFAG"
     gene            5506..7101
                     /locus_tag="AC16QF_14150"
     CDS             5506..7101
                     /locus_tag="AC16QF_14150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR6449752.1"
                     /translation="MTTYDVAIIGGGHNGLVCAAYLAGAGLDVIVLEKRGVVGGAAVT
                     EEFHPGFRNSVASYTVSLLNPKVIQDLRLAEHGLRIVNRPYSNFMPLPDGDYLRTGAT
                     IEETQQQFARFSRTDAERLPGYYAALEAGADLLRGMLLETPPNAGGGLRDVMRAVRLG
                     GRLRRLSTAARRDVLDLFTKSAGELLEYWFESDPVKALFGFDAVVGNYASPYAPGSAY
                     VLLHHVFGEANGQAGQWGHAIGGMGAITQAMLKEALARGVTVRTDADVRLVRVRNGRA
                     NGIVLEDGEEIGARAVASNLNPKLLFTRLIEAEALDDDFRARIARYRCGSGSFRINVA
                     LSQLPRFECAPPGQPELGTRSGIIMAPSLKYMERAYFDAREHGWSRKPIVEMLIPSTV
                     DDSLAPPGQHVASLFCQQFAPTLDGQSWAEARDAAAQTVFDTVEGYAPGFRQSILGAM
                     VLSPEDLETEFGLTGGDIFHGALSLDQLFSARPVLGHGDYRAPVRGLYMCGSGTHPGG
                     GVSGAPGHNAAREIIRDFRARRL"
     gene            7207..8865
                     /gene="oxc"
                     /locus_tag="AC16QF_14155"
     CDS             7207..8865
                     /gene="oxc"
                     /locus_tag="AC16QF_14155"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF006721.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HIR6449753.1"
                     /translation="MMATLDGTTLIARSLKQQGVEYMFGVVGFPVFGIAAAAQAEGIT
                     YIGMRNEQSASYAAQAAGYLTGRPQACLVVSGPGVIHAFAGLANAQQNCWPMILIGGA
                     SPTYQNGMGAFQEERQVELAKPYCKYAHGIDSVARIPYYVEQAIRTSLYGRPGPVYLD
                     LPDDIIRGTVEEDELVSAAPVGPAPRSQARPEDVEAALCVLESAERPLVVVGKGMAWA
                     RAENEVREFIERTQLPFLASPMGKGVLPDDDPLSVGAARSTALKEADVVFLMGARLNW
                     IMHFGLPPRFAPDVRIIQLDIAAEEIGTNVPAEVALLGDGQAVAAQLNAALDNRQWFY
                     PADAPWRTLLREKASDNQAAVAPMIDAEAMPMNYYRAYRDIEAWLPEDAIIIGEGANT
                     MDIGRTQMANRSARNRLDAGTYGTMGIGLGFTIAAAVVHPDRPVVAVQGDSAFGFSGM
                     ELETACRYKLPIKVIVLNNGGIGAGIDELPEDGPIPPFSLTPGARYEGIMETLGGRGL
                     YVEDPKDLRGALDEAMAFDGPALVNVRIDPRAGRKQQQFGWLTT"
     gene            complement(8867..10126)
                     /locus_tag="AC16QF_14160"
     CDS             complement(8867..10126)
                     /locus_tag="AC16QF_14160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450 family protein"
                     /protein_id="HIR6449754.1"
                     /translation="MTAIDRTVFECLRFTTAVADRLVTGSRMSGPAFLENPYGEYARL
                     RARGEVIRNFSIGGWNVLGFEAVRDAMRDARFGVDVRKSPFVDRIVRLVSRGRPFRML
                     DEPTMLNIDPPDHTRLRRLARQGFLHRYVCALEPKIEGLVEECLATVQGGMFDLMDTL
                     ARPLPANVIADILGVPREDRARFQAWSELIAKNGRNIEYDALQQTDAVFNELIDYLAQ
                     ITDAKRADPGDDLISHLIAAEEDGDRLSADEIHATSALLLIAGHETTTRLIGNAMWLL
                     LSHPEQLEILRANPALMPNAVEEALRFEAPVQHMTRIALEDMDFHGVHIRKHQLCEVY
                     IGSANRDPQANPNPDVFDICRENIQHVGFGYGIHLCLGAQLARLETQMALKMLLERFP
                     RLALTGAPPAWGDSTFVRGLDELLLAA"
     gene            complement(10260..11768)
                     /locus_tag="AC16QF_14165"
     CDS             complement(10260..11768)
                     /locus_tag="AC16QF_14165"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HIR6449755.1"
                     /translation="METPLFLELLNLLASIFVFLLGLGLIAVIVIYIVDRTQTKHAVR
                     RNFPVIGRFRYVFEHLGEFFRQYFFALDREEMPFNRAERTWVYRAAKGEDTTVAFGST
                     RDLRPPGTVLFVNCAYPTLDEDAAPLGRVTIGPDCEHPYDCGSLFNISGMSFGAVSRP
                     AVTALSNGARMAGVWMNTGEGGLSSYHLDGGADIVFQIGTAKYGVRNERGELCDEKLA
                     EMAAHRQVKMFEIKLSQGAKPGKGGILPGNKVTDEIARIRLIPVGSDSISPNRHPEIE
                     NEGDLLDMIDRVRRATGKPVGFKTVIGAYGWLEDLFAEINARGPASAPDFITIDSADG
                     GTGAAPMSLIDYMGLPIRESLPLVVDLIEAYGLRERIRVVASGKMVTPGEAAWAVCAG
                     ADFVVSARGFMFALGCIQALQCNKNTCPTGVTTHDPDLQRGLDPVDKAERVRRYAQHM
                     HRQLGMIAHSCGVAEPRQLKRFHCRIVTQTGISVPLDELYPPLVRDPARLSA"
BASE COUNT         2123 a         3881 c         3830 g         2104 t
ORIGIN      
        1 ttccgtctgg tcgaagtcac tggcaatgaa gccgtccgtc tccatccatc ccgtgaccga
       61 cgtcagcgtg aaggagccga tgtcccagtt gatgatcccg gtgatgctgt tctgatcggt
      121 gttgtggaag tccagggcgt cacggcccac ggtgaacgcg ccgtcgtctg gctcggtgaa
      181 gatgcaggac agaatctggg tcgggcaggg gtccgagcgg tcgtcgccgc ccggcgcgtc
      241 gctgcgctcc tccagccatg tcccgatcag ggtcgcttcg aaggtgtccg tgggcgtcca
      301 gacaatggtg ccgcggatgg tatcgatgtc ctcgccgttg agatcttcgc cgttcacccg
      361 gttcttgaaa tgaccgtcgt agttctgcat gagcagggaa atccgcgccg acagcttgtc
      421 ctcgatgatg ggaaactgaa tggcaccccg aaaatccaac cggccttcgt tgccgccggt
      481 gagctcggct gcgtagtcga aggatccgtc cgggcgtttg gtggtgacgg ccagaccgcc
      541 agccagcgag ttcttcccga acgtcgtgcc ctggggcccg cgaagcacct caaccgtctc
      601 cacgtcgaag aagtcgatca gggtagcgat ggggcgggat atgaagaccc cgtttatgga
      661 caccccggtg cgcatctcgt tggtggactc gatgttctgc tgtcccatgc cgcgtatgtg
      721 gatagccgca gaatttggaa aggtcaccac gggctggatg tgaacgttgg gcgatgcggg
      781 cccgacgtca cgcagatcct gggccgtaat ctgctccagc atgaccgagt tgaaggcggt
      841 caccgaggtg ggggattcct gagccgtttc ctcccgataa cgggcggtca ccaccacttc
      901 ctcgatcggc cgcgccgatt cttcgcccag cgccggcgcc ggggcaagcg ccaccagaat
      961 cgcaaccagg gcaagggtca gcaacgccgc gtcagcttca atcttcatcg tctatctcct
     1021 tacaatgatc ttgagcgcgc gcacaccctc ctctcgcaac ttctgcgtgc ggcgtgtttt
     1081 tcaggcgagc gtcaggccac gctgacgggc tcgcagttca cgtcgcgctc atcgagctcc
     1141 cgggtccagg cggcaaactc gaggagaatg ccgtcggggt ccttgaagta gatggagcgc
     1201 acgaaagtgg attccgtcac cgtggggctc gcctgggttt ccgagtcgtc atggttgacg
     1261 actgcggtaa catcgatgcc cgcagcagca agcttttcct gatactcctc gatcttctcc
     1321 tccggcacgt cgaacgccag atgattcatg gatgcatggg ccgacgtgat gtcgccccgg
     1381 cccacgagat ccggggcgtg agtgacgccg ggctggctgt cgccagcatt gggaaaccag
     1441 aagaacgcca gggaatcgcc cttgccgatg tcgaagaaga agtgctggcc catgccgttg
     1501 ggcagctcga tggtcttggt cagcggcatg cccagaacgc ccgaatagaa gtccaccgtt
     1561 tccttcatgt cccggcagac cagggcgatg tggttgattc cccgaatctc gaatttttcg
     1621 ttcgtctgca tctttccctc ctcctgttgg gctacgtccg gcccgcgccg gctgatcgta
     1681 atcagcgtca atatcattga ctatccgggg agttgtcaat agcatttttc cagcaaaaac
     1741 tgaatacagg ggaggatgaa atgccaagac tgaaacaggt ttcccgggcc gaagcgaccg
     1801 agaacgtgcg caaatattac gacgcgcttt tcggggagcg cgacccggtc gcggaacccg
     1861 gtacggcaac gggcacgccg ggcaactggt ggacggtctt cgccctggta ccctacgtgt
     1921 tcgatcatgc caccagccat ttcggcatgt tcgggatgtt cgccgacaaa agcatcagcc
     1981 agctcgacgg caaggtcagg gagctcggca tcatgcgcgc cgggtacacc cagggcagcc
     2041 agttcgtatt ttctcagcac tgcaaggcgg cgcggcgctt cgggatcagc gacgagcaga
     2101 tcgaagccat tccccattgg gaggtctcgg atgccttcga cgccaaggag cgcgccgtgc
     2161 tggcgtgggc cgacgcgctg attcttcagg gcgggagggc gtcggatgcg ctgttcgagg
     2221 ccttgcactc ccacctctcg gacgaggaca tcctcgagct cacctatcac atcatgggct
     2281 acaacctgca cgccgtctgc tgcaaggctc tgcggctgga gtacgacgac gttccggagc
     2341 gcattcgcga ggtgccggtt cccgccgagg gagagacggc cgactgggcc ggtggggcct
     2401 ggcagcgcga agagtaagcc catgagcgac agcgacagaa ttcactacgg cggtctgcat
     2461 catctggcac tggtctgccg tgacatggag cggacggtgg acttctacac caacgtgctg
     2521 gacatgaagc taaagaaggg cttcgacctg gacggcggct acggccagca cttcttcttc
     2581 gacatgggcg gcggcaacga gctggcgttc ttctggttcc gcgatgcaaa ggacgctcaa
     2641 cccggggtcg ccagcgccgc acatctggtc ggaagcggag agggctcgat cacctcggct
     2701 cacggctcca tgaaccacgt cgctttcgac gtggctccgg atcagatcga ggcttatcgg
     2761 gaaaagctgg tttcgcgcgg cgtgaactgc acgcccctgg tcaaccacga cgacgtcgtc
     2821 accggagagg aatctcgacg cgggtcgggc atcagcgcga agacctggct tcgctcgttc
     2881 tacttctacg acccggacgg catcatgctg gagttctgcg cgaccctgaa caccggttcc
     2941 ccggaagtgg agctgccggt aaacgctgca gggatcaagg ccaacggcga gccgatcacc
     3001 ggcgcgtgac caaccgcgat cagacatcca cggcatcacc gctcgagcac gcctcgggac
     3061 ccgccctcct gcgcgagctg gtgcacgcca cctactggct ggacgacggg ctgcaggcct
     3121 acatgaagga gcacgcgggc ctatcgctcc cgcgcgccca gtccatgatg atggtctaca
     3181 ttgccgaggg cgtggatagc ccgtcagacc tcgcccgacg acttcgcgtg agcaagcagg
     3241 ccatccgcca gggcctcaag gagctgatgg ctaaggacat ggtcacgctg gaacccgatc
     3301 ccggcaaccg cagacagaag gtggtccgct tcaccaagca tggccgcgcg ctgcgacagg
     3361 tggcctggga aggacttcag aatctggaac gggaactggg gcgacgactc ggcggggacc
     3421 gcctggccgc cctgcatgat gcccttttcc tggattgggg cgacgtcccc ggagagccct
     3481 gatcagggct catccgggag ccgtctcatt tagtgaaggt tgccgcgggc aatccaacgg
     3541 tcatcgccag acacccgcga aaccagttgc cgcgttaccc ctgggtgatg cgctcgtaaa
     3601 tggccgtcag gatgaccgca atggccaggc catcgaacac accgctgagg ttggtcagta
     3661 tggcggtcag gtaatcaccg acagccggaa ttggccccaa accgtcggcc accactgcca
     3721 acgccacggc catcaacagc accatgaggc gacgttcctc gtccacaccc atgaaccccg
     3781 ctaccaggcc gagaatgacc agggcggcgg cggcataggg aatggtaaca aacgcgccga
     3841 caactgccag aaccagcgca acgatccgaa taatgtttac agcactcata ctcattagcg
     3901 agctcccctc actgtgaatt cacgtctact gcattgtttt cctcgcgctc ggactgcagg
     3961 cgcaactcga atttagcaca acacgtcaca cggccgcgaa cagatcgttg aactgctcgt
     4021 tattcgacgg cggcgatgct tcggatgccg ccgcggaaaa gatctgccac cgaccgggtt
     4081 accagagcgc catgaatccg gtctcgggtt gagatcggac ttccgagccg ggagaatcgg
     4141 agatccccaa acagccaatc cgctaatcag gaggtcggcc gtgctggaca ccatattctc
     4201 cgccaccaac gcactcgcga tgatcggttg ggccggcttg atcttgctcc cgggccggcg
     4261 gttcgtggtt cagattctgg cccgggtgct gatcccggcc atcatcgccg tgatctacat
     4321 cggcctgatg gtcggccatg tgggcagctt cccggagggt ggcgggttcg gctcgctggc
     4381 gggcatcgcg acgctcttca gcgtggacgc actgctcctg gccgggtgga ttcactacct
     4441 ggcattcgat ctgttcgtgg gttcctggga ggtgacggat tcccggaaag aaggcattcc
     4501 ccacctgctc ctggtgccct gcctggtctt gaccttcatg gccggtcccg ccgggttggc
     4561 tctttacttt gccatcaagt acggacgccg atcgctggcg agcgaggcag cacagccgag
     4621 ccaatgagcg aaagggggcg gcagccggta ttggcagggg aatcgccggt ggaggcaacc
     4681 ccgctgctca tgggcattcg cgcgcgccac gccctctggt tcgattgcgc catggggttt
     4741 gccgccctgg ccgtcgtctg cctgctgatg cttgccatgg acgagcgcgc cctgaacggg
     4801 gtaaacgtct gggccaagcc cctgaagttc tctatctccc tcgccgttta cttcgccacg
     4861 ctggcctggt tcgcgccgct catgcccgac ggctatttcg caggaaccgg cggtcgctgg
     4921 ctcagcgcca tccctgtggc ctgcgccgtc ttcgagatcg tctacatcat cgtgcaggcg
     4981 gccctgggtg agccatcgca cttcaacgtc tccacgacgt tccacagcac gatgtacacg
     5041 ctgatgggag gcggcgcctt catcctcgtt gccatttgcc tgtggatggg cctgatcatc
     5101 ctgagcgtcc accggatgtc gaacccctac gcgctctcgg tgggcctcgc cctcgtgctg
     5161 acgttcgttc tcggcggtgg cttcggtgcc tacctgggcg gccagaccgg gcactgggtg
     5221 ggcggcacgc tctccgacgc cgacggtctt tggcttgtga actggtcgcg ggacggcggc
     5281 gatctaaggg tgccgcactt tttcgggatg cacgccatgc agatcctgcc ggctctggcc
     5341 accctggtcc ctcaccgcat gacccgggac ggggcgaacg tcgcggtgct cgccatgacc
     5401 ctgctttacg ccgcctttgc cggctggacg ttccagcagg ccgtgagcgg tataccgttc
     5461 gccggctagc gattagagtg cccgtccacg ggggcgccgg agggcatgac aacgtacgac
     5521 gtagcgatca tcggcggcgg tcacaacggg ctggtctgcg cggcttatct ggccggcgcc
     5581 ggcctggacg tgatcgtcct ggagaaacgg ggcgtggtgg gcggggctgc cgtcacggag
     5641 gagttccatc caggcttccg caattccgtg gccagctaca cggtcagcct gctcaacccg
     5701 aaggtcatcc aggatctcag gctcgccgag cacggcctgc gcatcgtcaa ccggccatac
     5761 tcgaacttca tgccgctccc cgacggagac tacctgcgca cgggggccac catcgaggaa
     5821 actcagcagc agttcgcccg gttctcccgg acagacgcgg aacgccttcc cgggtactac
     5881 gccgcgctcg aggccggcgc cgatctgctg cgaggcatgc ttctggaaac cccgcccaac
     5941 gccggcggcg ggcttcgcga cgtgatgcgc gctgtccgtc tcggcggccg cctgcgccgc
     6001 ctctccaccg cagcgcgacg cgatgtgctc gatctgttca cgaaaagcgc cggcgagctg
     6061 ctcgagtact ggttcgagtc cgatccggtc aaggcgctgt tcggcttcga cgccgtcgtc
     6121 ggcaactacg cgagccccta cgccccggga tcggcctacg ttctgctgca ccacgttttc
     6181 ggcgaagcca acggccaggc gggtcagtgg ggacacgcga tcgggggcat gggcgccata
     6241 acccaggcga tgctcaaaga ggcgctcgct cggggagtaa cggtgagaac ggacgccgac
     6301 gtgcggctcg ttcgcgtccg caacggtcgt gcaaacggta tcgtgctcga agacggggaa
     6361 gagatcggcg cccgggcggt agcgtcgaac ctcaacccaa agctgctgtt cacccggctc
     6421 atcgaagccg aggcgctgga cgacgacttc agggcgcgta ttgcccgcta tcgatgcgga
     6481 tcgggttcct tccgaatcaa cgtggccttg agccagcttc cgcgcttcga gtgcgctcca
     6541 ccgggccagc cggaactcgg cacccggtcg ggcatcatca tggcgccgag cctcaagtac
     6601 atggaacgcg cctattttga cgcccgcgag catggctggt cccggaagcc catcgtcgag
     6661 atgctcattc ccagcacggt ggacgactcg cttgctccgc cggggcagca cgttgccagc
     6721 ctcttctgcc aacagttcgc ccccacgctg gacgggcaaa gctgggccga ggcccgcgat
     6781 gcggctgcgc aaaccgtctt cgacacggtg gagggctacg cgcccgggtt caggcagagc
     6841 attctcggcg ccatggtgct ctccccggag gacctcgaga ccgagttcgg gctcacgggc
     6901 ggggatatct ttcacggcgc cctgtccctg gaccagctct tctcggcccg gccggtgctc
     6961 ggccacggcg actatcgcgc ccccgtgcgg ggcctgtaca tgtgcggctc cggcacccat
     7021 cccgggggcg gggtgagcgg ggcaccgggc cacaacgccg cccgggagat catccgcgac
     7081 ttccgggcgc gccggctctg aacggacaca cctcgaagcg agaggggccg gcggccttgc
     7141 tatactcggc ccctcggggc acgccagagc caccgcagag aagcagataa caaagcgagg
     7201 gagggaatca tggcaacctt agacggcact acgctcatcg caaggagcct caagcagcag
     7261 ggcgtcgagt acatgttcgg cgtcgtgggc tttccggtgt tcggcatcgc cgccgccgct
     7321 caggcggagg gcatcaccta cataggcatg cgtaacgagc agtccgcgag ctacgccgct
     7381 caggcggccg gctacctcac cggccgtccc caggcatgtc tggtggtttc gggtcccggc
     7441 gtgatccacg ccttcgccgg gctggcgaac gcccagcaga actgctggcc catgatcctc
     7501 atcggcggcg cctcccccac ctaccagaac ggcatgggcg cgttccagga agagcgccag
     7561 gtggagctgg ccaagcccta ctgcaaatat gcccacggca tcgacagcgt cgcgcggatt
     7621 ccctactacg tggagcaggc cattcgaacg tcgctctacg gccggcccgg gcccgtctac
     7681 ctcgacctgc ccgacgacat cattcgcggc acggtggagg aggacgaact cgtcagcgcc
     7741 gcgcccgtgg gcccggcgcc ccgctcccag gccagacccg aggacgtgga ggcagccctc
     7801 tgtgtgctcg aatcggccga gcggccactc gtcgtggtcg gcaaagggat ggcatgggcg
     7861 cgcgccgaga acgaggtgcg cgagttcatc gagcggaccc agctgccgtt tctcgcctcc
     7921 cccatgggca agggcgtgct cccggacgat gatccccttt cggtgggagc cgcgcgcagc
     7981 acggcgctca aggaggccga cgtggtcttc ctgatgggcg cgcgcctgaa ctggatcatg
     8041 cacttcggcc tgccaccgcg gttcgctccc gacgtgcgga tcattcagct ggacatcgcc
     8101 gctgaggaga tcggcaccaa cgtccccgcg gaggtggcgc tgctgggcga cggccaggcc
     8161 gtggccgccc agctcaacgc cgccttggac aaccgccagt ggttctatcc cgccgatgcc
     8221 ccgtggcgga cattgctgcg ggagaaggct tcggacaacc aggcagccgt ggcgcccatg
     8281 atcgacgccg aggccatgcc gatgaactac taccgggcct accgcgacat cgaggcgtgg
     8341 cttccggaag acgcgatcat catcggggaa ggggccaaca ccatggacat cggccgcacc
     8401 cagatggcga accggtcggc ccgcaaccgt ctggacgcgg gcacgtacgg aaccatgggc
     8461 atcggcctgg gcttcaccat cgcggccgcc gtcgttcatc cggaccggcc cgtggtggcc
     8521 gtgcagggcg acagcgcttt cggtttctcc ggcatggagc tcgagaccgc ctgccgctac
     8581 aagctgccca tcaaggtcat cgtcctcaac aacggcggca ttggcgccgg catcgacgag
     8641 ctgcccgaag acgggcccat cccgccgttc tcgctgaccc cgggcgcgcg ctatgagggc
     8701 atcatggaga cgctcggcgg gcgcggcctt tacgtggagg atcccaaaga cctccggggc
     8761 gcgctggacg aggccatggc cttcgatggc cccgccctcg tgaatgtacg catcgatccg
     8821 cgggccggcc gcaagcagca gcagttcggc tggctgacga cctgaatcag gcggccagca
     8881 gaagctcgtc gaggccgcgg acgaaggtgc tgtcacccca ggcggggggc gcgcccgtca
     8941 gggcgagccg gggaaaacgc tccagcagca tcttcagcgc catctgcgtc tcgagccgcg
     9001 caagctgggc ccccaggcaa aggtggatgc cgtagccgaa gcccacgtgc tgaatgttct
     9061 cacggcagat gtcgaagacg tccgggttcg gattcgcctg gggatcgcga ttcgctgagc
     9121 cgatgtacac ctcgcacagc tggtgcttgc ggatatgcac gccgtgaaag tccatgtcct
     9181 ccagagcgat ccgcgtcatg tgctgcacgg gcgcttcgaa ccgaagcgct tcctcgacgg
     9241 cgtttggcat gagggccgga ttcgcgcgca ggatctcgag ctgctccgga tggctgagca
     9301 gcagccacat ggcgttgccg atcaggcggg tggtggtttc gtgaccggca atcagcagca
     9361 gggcgctggt ggcatgtatc tcatccgcgg acagccggtc gccatcctct tcggcggcaa
     9421 tcagatggct gatcagatcg tcgcccggat cggcccgctt ggcgtcggtg atctgcgcaa
     9481 gatagtcgat cagctcgttg aaaacggcgt cggtctgttg cagggcatcg tactctatgt
     9541 tgcggccgtt cttcgcgatc agctccgacc aggcctgaaa ccttgcgcgg tcttcccgag
     9601 gcacgcccag gatgtcggca atcacgttag ccggcagggg acgcgccagg gtatccatga
     9661 gatcgaacat accgccctgg accgtcgcca ggcactcctc gaccaggccc tcgatcttcg
     9721 gctccagcgc gcagacgtac cggtggagaa agccctgacg ggccagccga cgcagccggg
     9781 tgtggtccgg cgggtctatg ttgagcatcg tcggttcatc gagcattcga aacggcctgc
     9841 ctcggctcac gagccgcacg atcctgtcca cgaaagggct cttgcgaaca tcgacgccga
     9901 aacgcgcatc ccgcatggcg tctcggaccg cttcgaagcc caggacgttc catccgccga
     9961 tgctgaagtt tcgtatgacc tcaccccggg cgcggagcct tgcatattcg ccataggggt
    10021 tctccaggaa agcggggccg ctcatccggc tgccggtcac caggcgatcg gccacggccg
    10081 tggtgaagcg taggcactcg aacaccgtcc tgtcgatggc cgtcatggcg ggactcccgt
    10141 tagaagcgct ggcctgcgcc gacacctcat tgttgcaccc accgatgccc cgacaaggct
    10201 tcgaacgcga acctgtgcgc cgcctcacag attggaggcc cagcgcggac cagctccgac
    10261 tatgcggaca gccgggcggg atccctgacg agcggcggat acagctcgtc taaaggcacg
    10321 gaaattcccg tctgcgtgac gatgcggcaa tgaaagcgtt tgagctggcg gggctcggcc
    10381 acaccgcagg agtgggcgat catgccgagc tggcggtgca tgtgctgcgc gtaacgacga
    10441 acgcgttccg ccttgtctac agggtccaga ccccgctgca ggtcggggtc gtgcgtggtc
    10501 acgccggtgg ggcaggtatt cttgttgcac tgcagcgcct gtatgcagcc cagggcaaac
    10561 atgaagcccc gggcggagac tacgaagtcg gcacccgcgc acacggccca agccgcctct
    10621 cccggtgtga ccatctttcc gctggcgacg accctgatgc gctcgcgcag accgtacgcc
    10681 tcgatgaggt cgaccacgag tggaagactt tctcttatgg gaagacccat gtagtcgatc
    10741 aggctcatgg gagcagcccc ggtcccgccg tcggcgctgt cgatggtgat gaagtccggt
    10801 gccgaggccg gcccccgagc attgatttct gcaaagaggt cctcgagcca gccgtacgcc
    10861 ccgatcacgg tcttgaagcc caccggcttc cccgtcgccc gcctgacgcg atcgatcatg
    10921 tccagcaggt ctccctcgtt ctcgatctca gggtgacgat tggggctgat ggagtccgaa
    10981 ccgaccggga tcagacgaat ccgcgcgatc tcgtcggtaa ctttgttacc cggcaggatg
    11041 ccgcctttcc ccggtttggc gccctgggag agcttgatct cgaacatctt cacctgccgg
    11101 tgcgcggcca tctccgcgag cttctcgtcg caaagctcgc cgcgctcgtt gcgcacgccg
    11161 tacttggcgg tcccgatctg aaagacgatg tccgcccctc cgtcgaggtg gtatgaggac
    11221 agcccgcctt caccggtgtt catccacacg cccgccatgc gcgcgccgtt ggaaagggcg
    11281 gtgacggccg gtcgcgagac ggcgccgaag ctcatgccgg agatgttgaa aagagaaccg
    11341 cagtcgtagg ggtgctcgca atcaggcccg atggtgacgc ggcctaaggg agccgcgtcc
    11401 tcgtcgaggg tggggtaggc gcagttgacg aacagcaccg tgcccggcgg gcgcagatcg
    11461 cgcgtggagc cgaaggccac cgtggtgtcc tcgccctttg cggcgcgata cacccacgtt
    11521 ctctccgctc ggttgaacgg catctcctcg cgatccagcg caaaaaagta ctgtcggaag
    11581 aactctccga ggtgctcgaa cacgtaacga aagcgcccga tgacgggaaa attgcggcga
    11641 accgcatgtt tcgtctgggt ccgatcgacg atgtagatca cgatcaccgc gatcagcccg
    11701 aggccaagca gaaagacgaa gatgctggcg agcaggttca aaagctcgag gaataagggc
    11761 gtttccatgg gcagcctccc gatagccacg gaccgagaat agagcaccga aagcggacaa
    11821 tcgacccgtc ggacgccgcc cggactgggg gcgggatatc aggagctgca ggacaacatg
    11881 gagcagccca cccgcgttct tgcccagtcc ggccgccggg ccgcaaaccg ctcacgtg
//
LOCUS       DBNHLM010000306         3487 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_45676, whole genome shotgun sequence.
ACCESSION   DBNHLM010000306 DBNHLM010000000
VERSION     DBNHLM010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_45676"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /gene="oadA"
                     /locus_tag="AC16QF_14170"
     CDS             complement(<1..1547)
                     /gene="oadA"
                     /locus_tag="AC16QF_14170"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HIR6449756.1"
                     /translation="MSTKRPLGITDVVLRDAHQSLLATRMRVEDMLPIAGKLDQVGFW
                     SIESWGGATFDACIRYLGEDPWERMRKLKAAMPNTPQQMLFRGQNILGYRHYADDVVG
                     KFVERAAANGVDVFRIFDALNDIRNLRTAIRATLDVGRHAQGTISYTVSPVHTMEVWV
                     DLGKQIEDTGAHSIAIKDMAGLLKPYVAFELVSRLKESVSVPIHMQCHATTGLSTATY
                     LKAIEAGIDNVDTAISSMSMTYGHSPTETLVSILEGGDRDTGLDISLLEEIAAYFRQV
                     RKKYAKFEGTLKGVDSRILVAQVPGGMLTNLESQLREQNASDRLDEVLDEIPRVREDL
                     GFIPLVTPTSQIVGTQAVINVLMNERYKNVTKETAGVLKGEYGATPAPVNADLQARVL
                     NGESPITGRPADHLQPEVEALTGELQELAAERGFALAGDVVDDVLTYALFPQVGLKFL
                     ENRGNPDAFEPAPWVVEVPALEAGRVVPAPPAPPRASTYNVRVNGRQFTVEVSEAGAV
                     SSVKPAEP"
     gene            complement(1558..1752)
                     /locus_tag="AC16QF_14175"
     CDS             complement(1558..1752)
                     /locus_tag="AC16QF_14175"
                     /inference="COORDINATES: protein motif:HMM:NF016187.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HIR6449757.1"
                     /translation="MVQGLHLMLAGMATVFVFLSVLVGATVVMSRVLMRLQGEPGPTA
                     DEVAAIVAAIERHRASGPDR"
     gene            1955..2031
                     /locus_tag="AC16QF_14180"
     tRNA            1955..2031
                     /locus_tag="AC16QF_14180"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1989..1991,aa:Arg,seq:acg)
     gene            2082..2216
                     /locus_tag="AC16QF_14185"
     CDS             2082..2216
                     /locus_tag="AC16QF_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449758.1"
                     /translation="MNKDNRRFHGWRLIGVIMIVIIALAAVSFVVDWMVIGPLEGRVF
                     "
     gene            complement(2227..2793)
                     /locus_tag="AC16QF_14190"
     CDS             complement(2227..2793)
                     /locus_tag="AC16QF_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6449759.1"
                     /translation="MRLSKLNRDDLTADQRAVLDAIQSGPRKSKHGAIGLVGPFGVWV
                     RAPRVGLATQALGAAARFDSRLPENVKEVAICTVGAFYRAKFEFAAHRELALAAGVDS
                     GEIEKLRVGDLPAFRGAEDLAYRVATSLLNLHRLDSNTYTGALGQFGEEGLIELVTII
                     GYYCMVALTLNAFEVPLTSGMDDPFPDA"
     gene            3085..>3487
                     /locus_tag="AC16QF_14195"
     CDS             3085..>3487
                     /locus_tag="AC16QF_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449760.1"
                     /translation="MLHGVVAVHLTAALLLISLLPLAGWASPDSALIITNARILDGAG
                     SVIDNGTVHVLGERIAAVGAEPSSSDASIMIDAQGMTVMPGFIDTHRHLLLYSNAGSA
                     RQLRRYINRTLSRELSQTLAQGITTALSPGDH"
BASE COUNT          647 a         1149 c         1027 g          664 t
ORIGIN      
        1 ggttcggccg gcttgacgct ggacacggcg cccgcttcgg aaacctccac ggtgaattgc
       61 ctgccgttca ccctcacgtt gtaggtgctt gcccgagggg gtgcgggcgg cgccggcacc
      121 accctgccgg cttccaatgc aggcacttcc accacccagg gcgcaggctc gaacgcatcg
      181 gggttgcctc gattctcgag aaacttgagc cccacctggg gaaacaaagc gtaagtcagg
      241 acatcgtcca cgacgtcgcc tgccagcgcg aaaccccgtt ccgccgcaag ttcctgcagt
      301 tcgcccgtca acgcttccac ctctggctgc agatgatctg ccggacgacc agtgatcggc
      361 gactccccgt tgagcacacg ggcctgaaga tccgcgttga ccggggccgg cgttgcgccg
      421 tattccccct tgagcacgcc ggccgtctcc ttggtgacgt tcttgtagcg ctcgttcatc
      481 aggacgttga tgaccgcctg ggtaccaacg atctgggagg tgggcgtaac cagagggatg
      541 aaaccgagat cctcacggac acgggggatc tcatcgagca cctcatcgag ccggtccgag
      601 gcgttctgct cgcgcagctg gctctccagg ttcgtcagca tcccgccagg cacctgggct
      661 accagaatgc gcgagtccac gcccttgagc gtgccctcga attttgcgta cttctttcgg
      721 acttggcgga agtaggcggc aatctcctcc agcagcgaga tgtcgagccc ggtgtcgcga
      781 tcgccgccct cgagtatcga caccagcgtc tccgtaggcg agtgaccata ggtcatgctc
      841 atggacgaga tggccgtgtc cacgttgtcg atgccggcct cgatggcctt gaggtacgtg
      901 gccgtcgaca gccccgtcgt ggcatggcac tgcatgtgga tgggcacgct gaccgactcc
      961 ttcaggcggc tcaccagctc gaaggccaca tagggcttga gaagcccggc catgtccttg
     1021 atggcgatgg agtgcgcgcc cgtatcttcg atctgcttgc ccaggtcgac ccagacttcc
     1081 atggtgtgca cggggctgac ggtgtacgag atcgtcccct gggcgtgcct gcccacatcg
     1141 agcgtcgcgc ggatggcggt tcgcagattc ctgatgtcgt tgagcgcatc gaatatccga
     1201 aaaacgtcga ccccgttggc cgccgcgcgc tcgacgaatt tgcccacgac gtcgtccgca
     1261 taatgccggt aacccagaat gttctggccg cggaagagca tctgctgcgg tgtgttcggc
     1321 atggccgctt taagcttgcg catccgctcc cacggatctt cacccagata ccggatgcag
     1381 gcatcgaagg tcgcgccgcc ccaggattcg atggaccaga acccgacctg atcgagcttg
     1441 ccggcaatcg gcagcatgtc ctcgacgcgc atgcgggtag ccagcaggct ctgatgggcg
     1501 tcacgcagga ccacgtcggt gatccccaac ggtctcttgg tgctcatggg tacctcatca
     1561 tcggtccggg ccggaggcgc gatgcctttc aatggccgca acgatggctg ccacttcgtc
     1621 cgcggtgggg ccgggctcgc cctgcagccg catgagcacc ctggacatga cgaccgtcgc
     1681 gccgacgaga accgagagaa agacaaacac cgtcgccatg ccagccagca tcaggtgcaa
     1741 accctgaacc atcagatcag acatctccgg accgattgcg ccactaaaac tcgggttgcg
     1801 cattctgcat caggtgcccg ggcaaaggta gccgcagcgc cgcgctccta tgaactgtcg
     1861 gccctttctt gacggggttt ttcgccttcc agataatagc gcgccctcgc ggactggaac
     1921 ggcaagcgat acgccatttc cccgcctgga ccgcgcgccc gtagctcagc tggatagagc
     1981 gcatggctac gaaccatgag gccgggagtt cgaatctctc cgggcgcgcc atttccatca
     2041 ccgaaaccgg ttccgaccgg aagacgatga gctctgcccc gatgaacaag gacaacaggc
     2101 gatttcacgg ctggcggctc atcggcgtca tcatgatcgt cataattgcc ctcgcggcgg
     2161 tcagcttcgt cgtggactgg atggtcatcg gtccgctgga aggccgcgtc ttctgagctt
     2221 gcgcggctaa gcgtccggaa acgggtcgtc cattccggag gtcaggggta cttcgaaagc
     2281 gttcagggtc agggcaacca tgcagtagta gccgatgatg gtgacgagct cgatgagccc
     2341 ctcctcacca aattgtccga gcgccccggt gtaggtgttc gagtccagcc gatgcagatt
     2401 cagcaggctc gtcgccacac gatatgcaag atcctccgcc cctcggaacg caggcagatc
     2461 ccccacgcgc agcttctcga tctctccgga atcgacgccg gccgccaaag cgagctcccg
     2521 gtgcgcagca aactcgaact ttgctcgata gaaagcgccg acggtgcaga tggccacttc
     2581 cttcacgttc tccggaagac ggctgtcgaa tcgtgccgct gcacccaggg cctgagttgc
     2641 gagccccacg cgcggcgccc ggacccagac gccaaacgga ccaacgagcc cgatggctcc
     2701 gtgtttgctc ttgcgcggcc cgctctgaat ggcatcgagc acggcccgtt gatccgcggt
     2761 caggtcgtcc cgattcagtt tcgacaatct catgaggcac ctcccctttc tccgggatca
     2821 tcttcgttca aacgccgtga aatatcgaat ggccccaggc gcgggagccg cgactttcaa
     2881 cacgtaacaa ttggttacct tatctccgtt gactgccatg gtgggggtgc ctatggtcct
     2941 gcccgcagcg tcgcttcacg gcttcgcacc aagcccgaaa ccacagctaa cggaccgcca
     3001 agtgatgatg cacagcaacg agactgcccg cgacgactcc gtctttgaga atgtcgttgg
     3061 agtcatggcg gccgtgtctg tgctgtgctt catggggttg ttgctgttca tctgacggcc
     3121 gcactactcc tcatatcact cctaccgctg gccggatggg ccagtcccga ttccgccctg
     3181 atcatcacca atgcccgcat cctcgacggt gcgggcagcg tcatagacaa cggcaccgtg
     3241 cacgttctgg gtgagcgaat tgcggccgtt ggggccgagc catcaagcag cgacgcatcg
     3301 atcatgatcg acgcgcaagg catgaccgtc atgcccggct tcatcgacac ccatcggcat
     3361 ctgctgcttt acagcaacgc aggcagtgcg cgacagctgc gtcgctacat caaccgaacc
     3421 ctttcccgcg aactctcgca gacgctggct cagggaataa ccaccgccct ctcgcccggc
     3481 gaccacg
//
LOCUS       DBNHLM010000307         5707 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1123656, whole genome shotgun sequence.
ACCESSION   DBNHLM010000307 DBNHLM010000000
VERSION     DBNHLM010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1123656"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="AC16QF_14200"
     CDS             complement(<1..861)
                     /locus_tag="AC16QF_14200"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6449761.1"
                     /translation="MFNATRRSTGCRALTLTTLAVMLSLLASGASAETTEQPSDDVLL
                     EEMVVTVERRSQNLQDYAGTAAAFTGEELKSLGVQNIQDLDRFLPGFSVANNGGNIEV
                     YIRGIGSSNNTELGDPAAATHLNGVYVPRPSGFGSAFFDIERVEVNIGPQGTLRGRNA
                     TAGSVDIIPWKPGLGITDASVEASIGNYGEWSVEGMVNVPVGEISALRVAGFILEHDS
                     YYDDKGPADVGVAEAAENWGGRLTYLIEPNDQWRFTLTGDYISEQGTGYTGTNYANPL
                     GNGIDPSDIKN"
     gene            complement(1176..2594)
                     /locus_tag="AC16QF_14205"
     CDS             complement(1176..2594)
                     /locus_tag="AC16QF_14205"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6449762.1"
                     /translation="MAEQAAHQELTPQEEIRGLIERSRAAQAQIENYTQEQVDELIRA
                     MVWCVAKPGVAEEIAQFTVDETQLGNYDGKYLKIHRKTRATLMDIINDKSVGILEEDK
                     ERNIVKIAKPMGVIGALAPSTNPEATPVIKAIHAVKGRNSIIVAPHPRAKLTNKMICD
                     KMREAIAACGAPADLVIAIESPSLELTNELMRQCDRVLATGGGAMVHAAYSSGTPALG
                     VGVGNAVITVDETADLEDAAEKIRLSKTLDLAASCSADNSVILVDAIYDEMMNKLEHQ
                     GGYLCNAEEKAKLQSTLWDDEGHLNTAIVAQPAEKIAGMAGIELPEGKRFFIVKETGW
                     GPEHPFSGEKLSVIMALYRARDIDHAIELTNNIQSYQGQGHSCGIYSTSDDNIMKLAN
                     ATKTSRVMVNQPQAASNSGNLWNGMRQTFSLGCGSWGGNGTNHNISWRDLINETWVSK
                     PLAKTKELPSDEELFGDVMGRI"
     gene            complement(2689..4308)
                     /locus_tag="AC16QF_14210"
     CDS             complement(2689..4308)
                     /locus_tag="AC16QF_14210"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6449763.1"
                     /translation="MSAHRIEVTTLGDLLLRAADRHGGRDAIIFPEARLTYDQLREGA
                     YARARSLLALGVDRGDHVGILMPNCLDYVECLFAVQLLGAMAVPINARYRARELAYVV
                     ENADLSAILISDVISEYADFAELLQEAFPELSANADPRALELPGAPKLKTVVMLGSSA
                     PAGILPRAELDALASGYQVEDVEARRVCARVRDQAIMMYTSGTTSDPKGCPLSHEVLV
                     RNGFNMNRERYFLDETDCFWAPLPMFHMASILPMICCMDAGAALQSMTHVEAGGALAM
                     MERDATTVAFPSFPTVTNELINHPEFTTRDLSRIRRINNVAPPDVLRKFQDAFPQAVQ
                     TGAYGLTEVGGVIAFNHPDESLEQRLTTCGRPFPGVEVKVVDPDTGAELGPGERGEIL
                     VRGYAVFDGYYKAPEKNAEAFADGWFRTGDLCALDADGSIEFHGRIKDMLKVGGENVA
                     ALEIESYLATHPAVKLAQVIGVPDERLLEVPAAFIELNPNAFATAQELIDYCKGKIAS
                     FKVPRHIRFVDEWPMSSTKVQKFRLLEQFEA"
     gene            complement(4340..5029)
                     /locus_tag="AC16QF_14215"
     CDS             complement(4340..5029)
                     /locus_tag="AC16QF_14215"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6449764.1"
                     /translation="MSRVLISGASRGIGLELVKQYAAAGDEVIACVRDTGNAALLDEV
                     ASGADNVTVQQMDAGDPASIEAAAAAIGDAPIDILINNAGAVGGQHQSLDDVDIAEWH
                     RTLDINTIGPLLISRAFKSNLAASGDGRLMIVTSQLAASTWPMGGMYIYSTTKAGVSK
                     VGQILALDWKEEPITVGLMHPGWVQTDMGGAHAEITAEESASGIRSVIAGMTKADSGS
                     FYKWNGEIHPW"
     gene            complement(5057..5470)
                     /locus_tag="AC16QF_14220"
     CDS             complement(5057..5470)
                     /locus_tag="AC16QF_14220"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR6449765.1"
                     /translation="MTSSADDIAAIEKLQADWNAAVEAGSVDGYAAVLDDDIELLPTD
                     AETIRGIGNYRAMLDGVFTTDDFRIEIAVPATIEVDGDMAWSRYDYVIHRTAKDGSGT
                     FSSYRKFLDVMRRQDDGTWRVYKHIWNYNDPDVVP"
     gene            complement(5512..>5707)
                     /locus_tag="AC16QF_14225"
     CDS             complement(5512..>5707)
                     /locus_tag="AC16QF_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6449766.1"
                     /translation="IRGAGLDVYEGEPDVPAGLLELDNVILLPHLGSATEETRTAMGM
                     RVAENLAAFFAGSEPPDRLV"
BASE COUNT          897 a         1802 c         1863 g         1145 t
ORIGIN      
        1 gttcttgatg tcagaaggat ctatcccgtt gccgagcggg ttggcgtagt tggtgcccgt
       61 gtagcctgtg ccttgctcgc tgatgtagtc gccggtcagc gtgaatcgcc actgatcgtt
      121 tggctcgatc aggtaggtga gtcggccgcc ccagttctcg gcggcttcgg ccacgccgac
      181 gtccgccggc cctttgtcgt cgtagtagga atcgtgctcg aggatgaaac cggccacccg
      241 cagagcggag atctcgccga cgggtacgtt caccatgcct tcgaccgacc actcaccgta
      301 gttgccgatg gatgcctcga cggatgcgtc ggtgatgccc agaccgggct tccagggaat
      361 gatgtccacg gagccggcgg tggcgttccg gcctctgagc gtgccctggg gcccgatgtt
      421 gacctcgacc cgctcgatgt cgaagaaggc ggagccgaac ccgctcggac gcgggacgta
      481 gacgccgttg aggtgcgtgg cagccgccgg gtcaccgagc tcggtgttgt tgctggagcc
      541 gatgccgcgg atgtagacct cgatgttgcc cccgttgttg gccaccgaga atcccggcag
      601 gaagcggtcg aggtcctgga tgttttgaac gcccagagac ttcagctcct ccccggtgaa
      661 tgctgccgcg gtgcctgcat agtcctgcag gttctgagac cgccgttcca cggtgacgac
      721 catctcctcg aggagcacgt cgtcggatgg ctgctcggtg gtttccgctg aggctcctga
      781 agccagcaag ctcagcatga ccgccagggt cgtcaatgtc agggctctgc agccggtgct
      841 gcgtcgtgtt gcgttgaaca tgatttgagg atccccctcg cgtttattcc gggtcaactc
      901 ctggggcatc ggattggcgt cccttgtgga gtgcgcgatg acatcgctgt cataatcgcg
      961 gtggaacagg atatacacag ttatgacagc gctgtcaaat ttgtctccgg aattgtgcgg
     1021 gcgcgcatcg ggtccccgcc cggggccgct cgtggggacg acggcggcct tcccacttgc
     1081 gtttgatgtt ttgctggaga ggtccgcaaa aaaaacgggc gtccacagga cgcccggttg
     1141 attggtgatg gcggggcccc ggtcggcccg ggccgctaaa tccggcccat cacgtctccg
     1201 aacagttcct cgtccgacgg cagctccttg gtcttcgcca ggggcttgga cacccaggtc
     1261 tcgttgatga gatcccgcca ggagatgttg tggttggtgc cgttgccgcc ccaggacccg
     1321 cagcccaggg agaacgtctg acgcatgccg ttccacaggt tgccgctgtt cgacgctgcc
     1381 tgaggctggt tgaccatgac ccgtgaggtc ttggtggcgt tggccagctt catgatgttg
     1441 tcgtcgctgg tggagtagat cccgcaggaa tgcccctggc cctggtacga ctggatgttg
     1501 ttggtcagct cgatggcgtg atcgatgtcc cgggctctgt agagcgccat gatcaccgac
     1561 agcttctcgc cggagaaggg atgctccggg ccccagccgg tttctttaac gatgaagaag
     1621 cgcttgcctt ccggcagctc gatgcccgcc atgcccgcga tcttctccgc cggctgggcg
     1681 acgatggccg tgttgaggtg cccctcgtcg tcccacagcg tgctctgcag cttggccttt
     1741 tcctcggcgt tgcacagata gccgccctgg tgctccagct tgttcatcat ctcgtcgtaa
     1801 atggcgtcca ccaggatcac cgagttgtcg gcggagcagg aggcagcgag gtctaaggtc
     1861 ttggacaggc gaatcttctc tgcggcgtct tccagatcgg ccgtctcgtc gaccgtgatc
     1921 accgcgttgc ccaccccgac acccagggcg ggcgtgccgc tcgagtaggc cgcgtggacc
     1981 atggcgccgc cgcccgtggc tagcacccgg tcgcactgcc gcatcagctc gttggtaagc
     2041 tccaggctgg gggattcaat ggcgatgacc agatccgccg gcgcgccgca ggcggcgatg
     2101 gcctcgcgca tcttgtcgca gatcatcttg ttggtgagct ttgcccgcgg atgaggcgcg
     2161 acgatgatgg agttgcgccc cttcacggcg tgaatggcct tgatcaccgg cgtggcttcc
     2221 gggttggtgg atggcgccag ggcaccgatc acgcccatgg gcttggcgat cttgacgatg
     2281 ttccgttcct tgtcctcctc gaggatgccg accgacttgt cgttgatgat gtccatcagc
     2341 gtcgcgcggg tctttcggtg gatcttcagg tacttgccgt cgtagttacc gagctgggtc
     2401 tcgtccacgg tgaactgggc gatctcctcg gccacgcccg gcttcgctac gcaccagacc
     2461 atggcccgga tgagctcgtc cacctgctcc tgggtgtagt tttcgatctg cgcctgcgct
     2521 gcgcgcgagc gctcgatgag cccgcgaatc tcttcttgcg gggtcaactc ttgatgggcg
     2581 gcctgctcag ccatagcgga atctcccttg acaacgatgg tggcccggat gcgggcccgc
     2641 cgaaccgggt actgtacaca accgtatctg gcctgacaaa agcctttctc aagcttcgaa
     2701 ctgctccagg agccggaact tctgtacctt ggtggacgac atgggccact cgtccacgaa
     2761 gcggatgtgg cgcggaacct tgaagctcgc gatcttccct ttacagtagt cgatcagctc
     2821 ctgcgcagtg gcgaaggcat tggggttcag ctcgatgaaa gcggcgggca cttccagcag
     2881 ccgctcgtcg ggcacgccga tcacctgggc gagcttcact gccggatgcg ttgccaggta
     2941 agactcgatc tccagcgccg ccacgttctc tccccccacc ttgagcatgt ccttgatgcg
     3001 tccgtgaaac tcgatggagc cgtcggcgtc aagggcgcac agatcgccgg tgcggaacca
     3061 gccgtcggca aaagcctccg cgttcttctc cggcgccttg tagtagccgt cgaaaacggc
     3121 gtagccgcgt acgaggatct cgccgcgttc tcccggtccc agctcggcgc cggtatccgg
     3181 atccaccact ttgacctcga cgccgggaaa tggccggccg caggtggtga gacgctgctc
     3241 caggctctcg tccgggtggt tgaaggcgat cacgccgccc acctcggtga gcccgtaggc
     3301 gcccgtctgc acggcttgag gaaacgcgtc ctgaaatttc cgcagcacgt ccggtggtgc
     3361 cacgttgttg atccggcgga tacggctcaa gtcccgggtg gtgaactcgg gatggttgat
     3421 cagctcgttg gtgaccgtcg gaaaggacgg gaaggcaacg gtggttgcgt cgcgctccat
     3481 catcgccagg gccccgccgg cctcgacgtg ggtcatggac tgcagggcgg caccggcatc
     3541 catgcagcag atcatgggca ggatggaggc catgtggaac atcggcaggg gagcccagaa
     3601 gcaatcggtc tcgtcgagga agtagcgctc gcggttcatg ttgaagccgt ttcgcaccag
     3661 cacctcgtgg ctgagcgggc aacccttcgg atccgaggtg gtgccggagg tatacatcat
     3721 gatggcctgg tcccgcaccc gggcacagac ccggcgggcc tccacgtcct cgacttggta
     3781 cccggacgcc aacgcgtcga gctcggcccg gggcaagatg ccggcaggcg ccgagcttcc
     3841 gagcatcacg accgtcttga gcttcggcgc gccgggcagc tcgagagccc ggggatccgc
     3901 attggcggac agctcgggga acgcttcctg cagcagctcg gcgaagtcgg cgtactcgga
     3961 gatgacgtcg ctgatcagga tggccgagag atcggcgttc tccaccacgt aagccagctc
     4021 ccgggccctg tagcgagcgt tgatcggtac cgccatggcg ccgagcagct ggacggcgaa
     4081 aaggcactcc acgtagtcca ggcagttggg catcaggatg ccgacgtggt cgccccggtc
     4141 gacgcccaga gccaacaggc tccttgcgcg ggcgtaggcg ccctctcgca gctggtcgta
     4201 ggtgagccgt gcctcgggaa agatgatggc gtcacggccg ccgtggcgat ccgccgcgcg
     4261 cagcagcaga tcgccaaggg tggtgacctc gatgcggtgt gcgctcaagc cgacaccttc
     4321 acgggtgcca ggaaacctgc taccagggat ggatctcgcc gttccacttg tagaagctgc
     4381 ccgagtctgc cttggtcatg ccggcgatca cgctgcggat gcccgatgcg ctctcctcgg
     4441 cggtgatctc ggcgtgggca ccgcccatgt cggtctgcac ccagcctgga tgcatgagac
     4501 ccacggtgat gggttcttcc ttccagtcca gcgccagaat ctgaccaacc ttgctcaccc
     4561 cggccttggt cgtggagtag atgtacatcc cgcccatggg ccacgtggag gccgcgagct
     4621 ggctcgtgac gatcatcagc ctgccgtcgc cggaagctgc caggttgctc ttgaacgccc
     4681 gggagatgag cagcggcccg atggtgttga tgtccagggt tcgatgccac tccgcgatgt
     4741 cgacgtcgtc gagggactgg tgctgtccgc ccacggcgcc ggcgttgttg atcaggatgt
     4801 cgatgggggc atcgccgatg gcagcagccg ccgcttcgat ggaggccggg tcgccggcgt
     4861 ccatctgctg aacggtgacg ttgtccgcgc cgctggcaac ctcgtcaagc agggcggcat
     4921 tgcccgtgtc ccgcacgcac gcgatcacct cgtcgccggc cgccgcgtac tgcttcacca
     4981 gctccagtcc aattccccgg ctcgcgccgc ttatcagtac tctcgacata tttgagtcct
     5041 ccctcttcga atttccctag ggtactacat cgggatcgtt gtagttccag atatgtttgt
     5101 acacccgcca ggtgccgtcg tcctggcgtc gcatgacgtc caggaacttc cggtagctgc
     5161 tgaacgtccc gctgccgtcc ttggccgtgc ggtgaatgac gtagtcgtag cgggaccagg
     5221 ccatgtcgcc gtccacctcg atggtggcgg ggaccgcgat ctcgatgcgg aagtcgtcgg
     5281 tggtgaacac cccgtcgagc atggcccggt agttgccgat gcccctgatg gtttccgcat
     5341 cggtgggcag cagctcgatg tcgtcgtcca gcacggcggc gtaaccgtcg acgctgccgg
     5401 cctcgacggc cgcgttccag tcggcctgga gcttctcaat ggcggcgatg tcgtccgctg
     5461 atgacgtcat ggttccccca acacgttgcc tgtgcgaagc gcgaaagttt atcagacaag
     5521 ccgatccgga ggctcgctcc ctgcgaagaa cgcggccaga ttttcggcga cccgcatgcc
     5581 catggcggtg cgcgtctcct cggtggcgct gcccagatgc ggcagcagga tgacgttgtc
     5641 gagctcgagc aggccggcgg gcacgtccgg ttcgccctcg tagacgtcga gcccggcgcc
     5701 gcggatt
//
LOCUS       DBNHLM010000308         2154 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_704524, whole genome shotgun sequence.
ACCESSION   DBNHLM010000308 DBNHLM010000000
VERSION     DBNHLM010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_704524"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /gene="nhaB"
                     /locus_tag="AC16QF_14230"
     CDS             complement(<1..602)
                     /gene="nhaB"
                     /locus_tag="AC16QF_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017679291.1"
                     /note="involved in regulation of intracellular pH under
                     alkaline conditions; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton antiporter"
                     /protein_id="HIR6449767.1"
                     /translation="MGGLSTASFWENFLGQAPRWYKTTIVGFLIVNPFLFMISEFVTG
                     WILVLEFIFTLAMALKCYPLQPGGLLAIEAVAIGMTSTDTIREEVFNNFPVILLLMFM
                     VAGIYFMKELLLFTFTKIILGVRSKTLVSLLFCAVAAILSAFLDALTVTAVIISVAVG
                     FYSVYHRVASGKSFRDEHEHGADESIGELHREDLESFRAFL"
     gene            864..1715
                     /gene="nudC"
                     /locus_tag="AC16QF_14235"
     CDS             864..1715
                     /gene="nudC"
                     /locus_tag="AC16QF_14235"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HIR6449768.1"
                     /translation="MWPIYPDEFESLVQAPPGEEDDRDAWYFLCINGQIACVVDRGAP
                     RPITADEFRWLDIEVFSKHYLGRFGNRPCFAMEGRGQLPPDGYLADSLYSWLGRVEPS
                     VFYLAGRAQQVVEWHNTHQYCGRCGAEMQEHAADRAKTCGGCGLICYPRIAPSIIVLV
                     TKGDEMLLARNARWPTGMYSTLAGFVEVGESIEQTVHREVYEEVGIQVDNLRYLGSQS
                     WPFPNSLMLGFHADYAGGEIICNEEEIADAKWFAHDELPSVPPATAISRWLIDDFIEE
                     VGRRSSG"
     gene            complement(1724..2056)
                     /locus_tag="AC16QF_14240"
     CDS             complement(1724..2056)
                     /locus_tag="AC16QF_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HIR6449769.1"
                     /translation="MPHDDLTRRLQSAFHPPAASHMDATFALDLGQLRISFRVRQGRL
                     DFVEGVPADATFYFDTPDEALQLLTGDADVFEAFMEGRFRADGYLMWTFALLSMFRGD
                     GAEPTPTG"
BASE COUNT          428 a          644 c          694 g          388 t
ORIGIN      
        1 aggaaggcgc ggaacgactc caggtcctca cgatgcagct caccgattga ttcgtccgca
       61 ccgtgctcgt gctcatccct gaaagacttt ccggaggcga ccctgtggta gacgctgtaa
      121 aagcccacgg caacgctgat gatcaccgcc gtcaccgtca gcgcgtcgag aaaggcggac
      181 aggatcgcgg cgacggcgca gaacagtaag gagacgaggg tctttgagcg aaccccgaga
      241 atgatcttgg tgaaggtgaa gagcagcagc tccttcatga agtaaatgcc cgccaccatg
      301 aacatgagga gcaggatgac gggaaagttg ttgaagactt cctcgcgtat ggtgtccgtg
      361 gacgtcatgc cgatcgccac ggcttcgata gcgagcagac cgccgggttg cagcggataa
      421 catttcaggg ccatggccag ggtgaagatg aactcgagga ccagaatcca cccggtgacg
      481 aactcgctga tcatgaaaag gaacggattg acgatcagga aaccgacgat ggtcgttttg
      541 taccaccgtg gtgcctggcc gagaaaattt tcccagaagc tggccgtgga tagtccgccc
      601 atctgctttt gcccggcgcg ccgggaaaac tgctccctgt aattgcctgc gaggtggccg
      661 cagcaaacca gcagaccttt gcctccccgg ccggtcctcc cttccggcca aaccggtggt
      721 cgagcgcacg ggtgtcagat cacgcttcga gaacattatc cggctcggca ggggagttca
      781 atacgatcgt ggccggaccc tgaacaggcc ggtgggtgtg ttatcgtttc ggctcatcgt
      841 cgataccacg agtcttgccc gccatgtggc ccatctaccc cgatgagttc gagtctctcg
      901 ttcaagcccc tcccggtgag gaggacgatc gggacgcctg gtacttcctt tgcatcaacg
      961 gtcagatcgc ctgcgtcgtg gaccgcggcg ccccccggcc gatcactgcc gatgagttcc
     1021 ggtggctgga catcgaggtc ttctccaagc actaccttgg ccgcttcggc aatcgcccct
     1081 gcttcgctat ggaggggcgc ggccagctgc cgccggacgg ttacctcgcg gacagccttt
     1141 actcgtggct agggcgcgta gagccgtcgg tgttctacct ggcgggtcgg gcgcagcagg
     1201 tggtggagtg gcacaacacc catcagtact gcgggcgctg cggcgcggag atgcaggaac
     1261 acgccgccga tcgtgccaag acgtgcggcg gatgcgggct gatctgctat ccgaggatcg
     1321 cgccgagcat catcgttctg gtgacgaaag gcgacgagat gctgctggct agaaacgcgc
     1381 gttggccgac gggcatgtac agcacgctgg cggggttcgt cgaggtgggc gagtccatcg
     1441 agcagacggt gcatcgggag gtctacgaag aggtcggaat tcaggtagac aatcttcgct
     1501 accttggcag tcagagctgg ccgtttccca actcgctcat gctgggcttt cacgcggact
     1561 acgcaggcgg cgagatcatc tgcaacgagg aggagatagc cgacgccaag tggtttgccc
     1621 acgacgagct gcccagcgtg ccccctgcta ccgccatctc ccgatggctc atcgatgact
     1681 tcatcgagga agtcgggcgc cgcagctccg gctgatgcgg ccttcagccg gttggtgtgg
     1741 gctctgcccc gtccccgcga aacatgctca gcagggcaaa agtccacatc aggtagccgt
     1801 ccgcgcgaaa gcggccctcc atgaaggcct cgaacacgtc cgcatcgccc gtgagcagct
     1861 gcagcgcctc atccggcgtg tcgaagtaaa aggtcgcatc tgccgggacg ccctccacga
     1921 aatcgagccg gccctgacgg acgcgaaaac taatgcggag ctggcccaga tcgagggcaa
     1981 acgtggcgtc catgtgggaa gccgccggcg gatgaaacgc gctctgcagt cgccgggtca
     2041 ggtcgtcgtg gggcatcgtt gaatcagaca cgcatgtgcg gatgagatca agccgtgcgc
     2101 atgtggctgc aggcgcggcg gtggacgccc gttgacgcag ccagtacatt agcg
//
LOCUS       DBNHLM010000309         2722 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_705012, whole genome shotgun sequence.
ACCESSION   DBNHLM010000309 DBNHLM010000000
VERSION     DBNHLM010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_705012"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..396
                     /locus_tag="AC16QF_14245"
     CDS             217..396
                     /locus_tag="AC16QF_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449770.1"
                     /translation="MTEIPEIPHANDNRWTKADLRAYVLEHLELAAQIERTRPLTFRE
                     RRARQILIDLLHRHG"
     gene            complement(595..1746)
                     /locus_tag="AC16QF_14250"
     CDS             complement(595..1746)
                     /locus_tag="AC16QF_14250"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 5 family protein"
                     /protein_id="HIR6449771.1"
                     /translation="MPHPVVSFATTLAGMARGAYPRRWTAPVPSIARWSIERARRWRE
                     DTGWLNGCNFVPSSAGNQLEMWQAETWDPETIEREIGWAADLGMNSLRVFLHDLLWTA
                     EGPAFLDRVDKFLTITERHGVSTMLVLFDGVWNPEPALGPQGEPTPRRHNALWVQGPG
                     RAILGDPGRWPELRPYVEAVVGRFKSDPRVAVWDLFNEPDQSNPLSYPRQEIFGKTRL
                     ADGLLNQVFDWCQAIDPEQPLTAGIYLGVNGAAERVSRLNRTMLGRSDVISFHSYAPR
                     SKLLGTIAYLERYERPLLSTEWLARTLGSSVSLIRDLADQDVDAWCWGLVDGRSQTRY
                     SWTSWLRRPADDSGPWFHDLLHADGRAYDEAEVALLRSVSTSKKRVPRI"
     gene            1872..>2722
                     /locus_tag="AC16QF_14255"
     CDS             1872..>2722
                     /locus_tag="AC16QF_14255"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6449772.1"
                     /translation="MNWYRIKTALIGLKRLATLPEEDKQACVDAYKFFQRMQAGEETS
                     TEDETKAVADYYRVLNNMLSVFDLEKLYIPPQLDDSQGLYGNQLLCEQAVLKELALEK
                     PDKSHLLDMGCGRGRISHYLATLTGGQVSGYNIDPNQIENAIDWAAETGMSDRLHFKV
                     GNHHEPLEYESEFFDGCFSFQAVWPFFKKEELDDHAREMYRVLKPGARYACSEYLLSP
                     YFDWNNEEHVALHKTYLPTLAATQSMYPADVCSALERAGFQILVSAPSKSEAWPLCEQ
                     KRDLILMG"
BASE COUNT          537 a          855 c          865 g          465 t
ORIGIN      
        1 cctccactac aggaacgctc atgatatggc ggcttgcggg ggcgccgcgc gcccaagggg
       61 cgctacgcgg gctgtgcgat cgcgaccccg cgacgacgct gcccagaaga ctgccgagct
      121 cgagcggaac gagcccgagc gggattgagg cgtgatttcg tcggcacttt ggccccgctt
      181 ctcagccagg gggaggagaa aaagcggagg ccaaagatga ccgaaatccc cgagatccca
      241 cacgcgaacg acaaccgctg gacgaaggcc gaccttcggg cctacgtgct cgaacacctc
      301 gagctggcgg cccagatcga gcgaacgcgc cccctcacct tccgcgaacg ccgcgcccgt
      361 cagatcctca tcgatctcct gcatcgacac gggtgaccat caggaatgcc gattgctggg
      421 ccgcaggggc tcgctgagag gcgaaaccgc tccgcggcac cctacccctc cattgctctt
      481 tcttgatggc tcctcgcgct tccccggcaa gaaatggcgg tcgcgccttg gtcacccccc
      541 gccggcgagc tcaggagtcg ttccgccacc tgaccgtaga cgtactctga atcgctagat
      601 ccgaggcacg cgcttcttgc tcgtagatac gctacgaagc agcgccactt ccgcctcgtc
      661 gtaggcgcgc ccgtcagcgt gcagcagatc gtggaaccac gggcccgagt cgtctgcggg
      721 gcgccgtagc caactcgtcc acgagtagcg agtctggctg cggccatcga ccagtcccca
      781 gcaccacgcg tcgacatctt ggtcggcgag atcacggatc agggagacgg atgagcccag
      841 tgtgcgcgcc aaccactcgg tacttagaag cgggcgctcg tagcgctcca gatacgcgat
      901 ggtacccaga agcttgctcc gcggtgcgta ggagtgaaac gagatcacgt cggagcgccc
      961 cagcatggtg cgattcagac ggctcacccg ctcggcagca ccgttcacgc cgagatagat
     1021 cccggccgtg aggggctgct ccggatcgat cgcttgacac cagtcgaaca cctggttcag
     1081 caagccgtct gcgagacggg tcttgccgaa gatctcctgc cgcgggtagc tcaacgggtt
     1141 ggactggtcg ggctcgttga agagatccca gactgcgacg cgggggtccg acttgaagcg
     1201 gccgacgacc gcttcgacgt aggggcggag ctcgggccag cgcccgggat cgccgaggat
     1261 ggcgcggccc ggcccctgca cccagagtgc gttgtggcgt cgcggggtcg gttcgccctg
     1321 ggggcccaga gcaggctccg gattccacac gccgtcgaag aggacgagca tggtcgagac
     1381 cccatgtcgc tcggtgatcg tgaggaattt gtcgactcgg tccagaaagg ccgggccttc
     1441 cgctgtccag agaagatcgt gcaggaatac gcgcaggctg ttcatgccga ggtcggcagc
     1501 ccacccgatt tcgcgctcga tggtttcggg gtcccaggtt tcggcttgcc acatctcgag
     1561 ttggttgccc gcactcgacg gaacgaagtt gcagccgttg agccagccgg tgtcctcgcg
     1621 ccaacgccga gcacgttcga tcgaccagcg tgcgatcgat ggcaccgggg cagtccaccg
     1681 gcgcgggtag gcaccacgag ccatgccggc aagagtcgtc gcgaacgaaa cgaccgggtg
     1741 gggcatgaca tcagtgtatc catagggacg ggcccacgtc tggctcatcc ggaaaggcgt
     1801 cccggagctg ctggatcacc cagcaggccg ctgggctacc ctacaggccc gatgaggcag
     1861 gagtgcacaa ggtgaactgg tacaggatca agaccgcgtt gatcggcttg aagcggctgg
     1921 caacgttgcc tgaagaggac aagcaagcgt gtgtcgacgc gtacaagttc tttcagcgca
     1981 tgcaggccgg tgaggagacc tcgacggaag acgagacaaa ggccgtcgcg gactattaca
     2041 gggtcttgaa caacatgttg tcggtcttcg acctcgagaa gctctacatc cctccgcagc
     2101 tggacgattc acagggtttg tacgggaacc agctgctgtg cgaacaggcg gtgttgaaag
     2161 agctggctct cgagaagcca gacaagtccc atctgttgga catgggatgc gggcgaggac
     2221 ggatttccca ctacctcgcc acactgaccg gaggtcaggt ctccggctac aacatcgatc
     2281 cgaatcagat cgagaacgcg atcgactggg ccgccgaaac gggaatgagc gatcggctcc
     2341 acttcaaggt cggcaatcat cacgagccgc tcgagtacga gtccgaattc ttcgatggat
     2401 gcttttcctt ccaagcggtc tggcccttct tcaagaagga ggagctcgac gatcatgcga
     2461 gagagatgta ccgcgtgctc aaacccgggg ctcgttacgc ctgctccgag tatctcctga
     2521 gcccctattt cgactggaac aacgaagagc acgtggcgct tcacaagacc tacctgccaa
     2581 cgctggccgc cacgcagtcg atgtatccgg ccgacgtttg ttccgctttg gaaagagccg
     2641 gcttccagat cctcgtttcg gcgccttcga agagcgaagc ctggccgctc tgcgagcaga
     2701 agcgcgatct gatcctgatg gg
//
LOCUS       DBNHLM010000310         4530 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_167024, whole genome shotgun sequence.
ACCESSION   DBNHLM010000310 DBNHLM010000000
VERSION     DBNHLM010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_167024"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="AC16QF_14260"
     CDS             complement(<1..385)
                     /locus_tag="AC16QF_14260"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6449773.1"
                     /translation="MAETNIQSAPQESRAEKLDAGHPQFLRGDPITGDRYYSQDFARR
                     EWDRMWTRVWHIAGRESELPDAGDWIVHNFLRESIIATRQSDGSIRAFYNSCQHRGNR
                     LVWAGEGRGNFTCSYHGWEYGTDGVL"
     gene            complement(406..1407)
                     /locus_tag="AC16QF_14265"
     CDS             complement(406..1407)
                     /locus_tag="AC16QF_14265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6449774.1"
                     /translation="MTVNTQVLIDSLPQGKLTADNYRIAESPVPEIGDGEVLVQTTAF
                     AITAGTRAGLQGSASYAGAPQTGVVMNGTGVGEVTESRSEDFAAGDRVLGPTGWQAYS
                     VHKPRALTLIPEGHDPVDYLGPLGVNGLTAYFGLFEVGRPAAGETVMISAAAGSVGHL
                     VGQMATTVDARVVGVCGSDAKGRILTDELGFDAVVNYREDGFRQALKEATPDGVDVYF
                     DNTGGDILGAALRRMNVAGRIACCGVVSQYDTDNPEPGPRGIPGLLINKRLDMRGFLV
                     FDFADRYDEARGNIAAWLESGKLKALVDEVDGVEAAPAAFVDLLAGGNVGTRVVRVT"
     gene            complement(1404..2699)
                     /locus_tag="AC16QF_14270"
     CDS             complement(1404..2699)
                     /locus_tag="AC16QF_14270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spinster family MFS transporter"
                     /protein_id="HIR6449775.1"
                     /translation="MSGARSHWSVGGRNYVLVLLTATAAFNFVDRQILGVLLEPIGQE
                     FDLPDSLLGLLSGIAFAVFYAVLGIPIAALADRLNRRNIIVVCIALFSTMTALCGAVV
                     NFWQLFLARIGVGVGEAGTMPASQSMLSDLFPVQERPWAMGLLATGGNIGLMLGLLIG
                     GWVNEWFGWRAAFVTAAIPGALIAAVIYFTVPEPVRRHAAAARSGWMREAFTGMRLVG
                     SIRSVRRFAMGGTLYGLSAYGIHTWMPVYYIRYHGLSTGEAGTVISIIVGVLGGIGAF
                     AGGVFCAKLSQRDLRWNGWLPALAIFTSVPLLIAMLITDNTWLAIGLFVLPGILSSVY
                     AGPTWSIIQELVPAQRRAMAASVYMLFFNLIGLGLGPLTVGVLSDLYIPAIGEASLRW
                     AMVSVLLVSLGGITAYVLAARSLIGDLDVLRSRAGGAQT"
     gene            complement(2731..3339)
                     /locus_tag="AC16QF_14275"
     CDS             complement(2731..3339)
                     /locus_tag="AC16QF_14275"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6449776.1"
                     /translation="MLILHHAPNSRAGRIVWLLEELGLDYELNRMDFHPRDLKSEAHR
                     ERHPLGRVPVLEDGEIMLYESGAIVEYIVARHSDGALKPSVDSPLFPEYLQWFHYCEG
                     MVMPPVNTIVVQTLLLPPERRNEEALGQAQRLLAKAVAPVDEALAGREYLIGDFSAAD
                     IMLGHALYMSRRLGAVSEEMTNLNAYIDRIEARPAFQKGIQA"
     gene            3487..>4530
                     /locus_tag="AC16QF_14280"
     CDS             3487..>4530
                     /locus_tag="AC16QF_14280"
                     /inference="COORDINATES: protein motif:HMM:NF025114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6449777.1"
                     /translation="MNDEPDVLVVGAGFAGMYAVHRLGELGLTVKVLEAGGDVGGTWY
                     WNRYPGARCDVPSLEYSYGFSPELEQEWDWPEVFSSQEDILAYANHVADRFDLRRHMR
                     FDTRIVAVEYREDENVWRLTSEAGEVFEAPFCVMATGCLSVPNTPRLPGADSFAGLVL
                     HTGRWPHEAVDLAGKRVGIIGTGSSGVQAIPELARQSGHLVVFQRTPVYTVPANRKAM
                     RPDVQREFREHYRSIRGMQQRNAGGVSGYRPLRGGRAGPAVPGERALRTGILDLDPDQ
                     RRRIVAERGLETLLNFPDVYTDPEANEVANALYRDAVRAEVRDPETAEKLLPKDYGLG
                     CKRQVLDRDFFETF"
BASE COUNT          782 a         1480 c         1538 g          730 t
ORIGIN      
        1 cgagcacgcc gtcggtaccg tactcccagc cgtgatacga gcaggtgaag ttgccgcgac
       61 cctcgcctgc ccacaccagc cggttaccac ggtgctggca gctgttgtag aacgcgcgga
      121 tcgatccgtc gctctgacgg gtcgcgatga tggactcgcg cagaaaattg tggacgatcc
      181 agtcgccggc gtcgggcagc tccgattccc gcccggcgat gtgccacaca cgcgtccaca
      241 ttcggtccca ctcacggcgg gcaaagtcct ggctgtagta gcgatcgccg gtgatgggat
      301 ctccgcgcag aaattgcggg tgtccggcgt ccagcttttc tgcgcggctc tcctgcggag
      361 ccgactgaat gttggtttca gccattccag gttcctcctc gacgttcagg tcacgcggac
      421 cacgcgcgtg cccacgttgc ccccggctaa cagatcgacg aacgcggcgg gcgcggcctc
      481 gaccccgtcc acttcgtcta ccagggcctt cagtttgccc gactcgagcc aggctgcaat
      541 attcccgcgc gcctcgtcgt agcggtcggc aaaatcgaaa acgagaaagc cgcgcatgtc
      601 caggcgcttg ttgatcagca accccgggat cccgcgcggg ccgggttcgg gattgtcggt
      661 gtcgtactgg gagaccacgc cgcagcaggc gatgcggccg gccacgttca tgcgccgcag
      721 ggcagcaccg aggatgtcgc cacccgtgtt gtcgaagtag acgtccacac cgtcgggtgt
      781 cgcttccttg agcgcctggc ggaagccgtc ttcccggtag ttgaccacgg cgtcgaagcc
      841 gagctcatcc gtgagaatgc ggcccttggc gtcgctcccg caaacgccca cgacccgcgc
      901 gtccaccgtt gtggccatct gcccgaccag gtggccgacg ctgccggcgg cggccgagat
      961 catcaccgtt tccccggctg ccgggcggcc cacctcgaag agcccgaagt aggcggtgag
     1021 cccgttgacg cccagaggcc ccagatagtc aacgggatcg tggccctcgg ggatgagcgt
     1081 cagcgcgcgc ggtttgtgaa cggagtacgc ctgccagccg gtgggcccga gcacccgatc
     1141 gcccgcggcg aagtcttcag agcggctttc cgtcacctcg cctacgccgg tgccgttcat
     1201 gaccaccccg gtctgcggcg cgccggcata gctggcgctt ccctggagcc ccgcccgggt
     1261 gccggccgtg atggcgaagg ccgtggtctg cacgagcacc tcgccgtcgc cgatctcggg
     1321 cacggggctc tcggcgatcc gatagttgtc agcggtgagc ttcccctgcg gcagcgagtc
     1381 gatgagcacc tgggtattga cggtcatgtt tgggcacctc cggcacgcga gcgtagcacg
     1441 tccagatcgc cgatgaggga cctcgctgcc agcacgtagg cggtgatgcc tcccaggctc
     1501 acgagcagca cgctcaccat ggcccagcgc agcgaagcct cgccaatggc gggaatgtac
     1561 aggtcgctga gaacccccac cgtcagcggc cccaggccaa ggccgatgag gttgaagaaa
     1621 agcatgtaaa ccgacgccgc catggcccgc cgctgcgcgg gcaccagttc ctgaatgatg
     1681 gaccaggtgg gcccggcata gacgctggag aggatcccgg ggagcacgaa caggccgatg
     1741 gcgagccagg tattgtcggt gatgagcatg gcgatcagga gcggcacgct ggtgaagatc
     1801 gccagggccg gcagccagcc gttccagcgc agatcgcgct ggctgagctt cgcgcagaag
     1861 acgccgccgg cgaaagcgcc gatgccgccg agcacgccga cgatgatgga gatcaccgtt
     1921 cccgcttcgc cggtggaaag gccgtggtag cggatgtagt aaacgggcat ccaggtgtgg
     1981 atgccgtagg ccgaaagacc gtagagcgtg cctcccatgg cgaatcggcg gaccgagcgg
     2041 atactgccca ccagccgcat cccggtgaag gcctcgcgca tccagccgga ccgcgcagcc
     2101 gccgcgtgcc tgcggacggg ctccgggacg gtgaagtaga tgactgcggc gatgagcgcg
     2161 ccgggtatcg ccgcggtcac gaaggcggcc cgccagccga accactcgtt cacccagccg
     2221 ccgatgagca ggccgagcat cagaccgatg ttgcctcccg tggccagcag tcccatggcc
     2281 cagggtcgct cctggaccgg aaagagatcg gagagcatcg attggctggc cggcatggtg
     2341 ccggcttcgc ccacccccac gccgatgcgc gcgaggaaca gctgccagaa gttcaccacc
     2401 gccccgcaca gagcggtcat ggtgctgaag agcgcgatgc acaccacgat gatgttgcgg
     2461 cggttgagac gatcggcgag ggcagcgatg ggaatgccga gcaccgcgta gaaaacggca
     2521 aaggcgatgc ccgaaagcag gcccagcagg ctgtcgggca ggtcgaactc ctgacctatg
     2581 ggctcgagca gcaccccgag gatctgtcga tcgacgaagt tgaaggcggc ggttgccgtg
     2641 agcagcacca gcacgtagtt gcggccgccg acggaccagt gtgatcgcgc gccgctcatt
     2701 ttccgctccg ggccctgtcc gcccgccgga tcaggcctgg atgcccttct ggaacgcggg
     2761 ccgggcttcg atgcggtcga tgtaggcgtt gaggttggtc atctcctcgg agacggcacc
     2821 caggcgccgg gacatgtaaa gggcgtgacc cagcatgatg tccgcggcgg aaaagtcgcc
     2881 gatgaggtat tcgcggcccg ccagggcctc gtcaacgggt gccaccgcct tggccagcag
     2941 tcgctgggcc tggccgaggg cctcctcgtt gcgccgctcg ggaggcagca gcagcgtctg
     3001 cacgacgatg gtgttcaccg gcggcatgac catgccctcg cagtagtgaa accattgcag
     3061 gtactcgggg aacagcggcg agtccactga gggcttgaga gcgccgtcgc tgtgccgcgc
     3121 gacgatgtac tccacgatgg cgcccgactc gtagagcatg atctccccgt cctctaaaac
     3181 gggtacgcgg ccgagcgggt ggcgttcgcg gtgggcctcg gacttgaggt ccctgggatg
     3241 gaaatccatg cggttgagct cgtaatccag cccaagctcc tcgagaagcc agacgatgcg
     3301 tccggcccgt gaattgggcg cgtggtgcag tatcagcact gaaatctctc cctccagacg
     3361 acgtcggcag tgtaacccga caggcatgga atatcgctgc gagatccctg cggggccggc
     3421 cggtgacgct gagcatcggc tgcccttaga atcaggtcat gtcaaagagg ggaatccggc
     3481 gcccggatga acgacgaacc tgacgtgctg gtggtgggtg cgggttttgc ggggatgtac
     3541 gccgtgcatc gcttaggcga gctcggcctc acggtgaagg tgctggaggc gggtggagac
     3601 gtcggcggca cgtggtactg gaaccgctac cccggcgccc gctgcgacgt tccgagcctg
     3661 gaatactcct acggcttttc cccggagctg gagcaggagt gggactggcc cgaggtattt
     3721 tcctcacagg aggacattct cgcctacgcg aaccacgtgg ccgaccggtt cgatctccgg
     3781 cgccacatgc ggttcgatac gcggatcgtc gccgtcgagt accgggagga tgagaacgtc
     3841 tggcgcctga ccagcgaggc gggcgaggtc ttcgaagcgc cgttctgcgt catggccacc
     3901 ggttgcctgt ccgtccccaa cacgccccgc ctgccgggtg ccgactcctt cgccgggctc
     3961 gtgctccata ccggacgctg gccccacgaa gccgtggatc tcgcgggcaa gcgggtgggc
     4021 atcatcggca ccggatcctc cggcgttcag gccattcccg agctggctcg tcagtcggga
     4081 cacctggtgg tgtttcagcg cacgcccgtc tacacggtcc cggcgaaccg caaggcgatg
     4141 cggcccgacg tgcagcgcga gttccgtgag cactatcgca gcatccgcgg catgcagcag
     4201 cgcaatgccg gtggggtttc cggatatcgg ccgctcagag gagggcgggc tggtcccgcg
     4261 gtgcccggcg agcgcgcctt gcgcacgggc atcctcgatc tcgaccccga tcaacgccgt
     4321 cggatcgttg ccgagcgagg tctggaaacg ctgctcaact tccccgacgt ctacacggac
     4381 ccggaggcca acgaggttgc caacgccctt taccgggacg cggtccgggc ggaggtgcgg
     4441 gatccggaga ccgcggagaa gctgctgccg aaagactatg ggctgggatg caagcgccag
     4501 gtgctggacc gcgacttttt cgagaccttc
//
LOCUS       DBNHLM010000311         4284 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1663673, whole genome shotgun sequence.
ACCESSION   DBNHLM010000311 DBNHLM010000000
VERSION     DBNHLM010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1663673"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..913
                     /locus_tag="AC16QF_14285"
     CDS             248..913
                     /locus_tag="AC16QF_14285"
                     /inference="COORDINATES: protein motif:HMM:NF018961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HIR6449778.1"
                     /translation="MEAVEDLLAHCESLLDVTVQAVDGIAEAQGVDLETRFASDRKDL
                     YRRYLAHCLDDKILTEDENADLQHLLNLLHLNPDDVVPVHDEMAREVYGKAIQEVLAD
                     LEVDADEEAFLRRLRGDLKLSDDVASDLLERGRRDAHDVALREASTPDHDFLVYRAPA
                     GEFTGRSDVSFEAAVTDALSKAVIAIPMLHWFEVSNISGYVGDGKPRGWHVTVRGGIE
                     PEK"
     gene            914..1729
                     /locus_tag="AC16QF_14290"
     CDS             914..1729
                     /locus_tag="AC16QF_14290"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR6449779.1"
                     /translation="MPRRPARWLLSGWCVTALLGLAACEPGHTPFPDGEIVDLSHTYD
                     ETTVFWPTADGFQLEVESAGYTDKGYYYAANNFSTSEHGGTHLDAPIHFAEGAPATDE
                     IPLERLVGPGVVIDVSAACTDDADYQIDVADVTRWERANGQTVDGMIVLFRTGFAERW
                     PDVARYLGTAERGAEAVAKLHFPGLHPELAAWLVAERDVKAVGLDTASIDYGQSTLYE
                     THQILFKAGIPAFENVANVSRLPASGFSVVALPMKIGGGSGGPLRIIAIVPPA"
     gene            complement(1734..3293)
                     /locus_tag="AC16QF_14295"
     CDS             complement(1734..3293)
                     /locus_tag="AC16QF_14295"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF017085.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050355 - inorganic triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HIR6449780.1"
                     /translation="MNELEAKYLVAAGRKPRRVLEHVLQTLSWAGYRARLRSERLVVD
                     TYFDTPDWALQRAGWAYRLREDEQKKTRTLAVKAIKTGHYGVFRREEFEQSLDAPLNR
                     PGDPPAGQVADQLWVIVTEEASLKPLFHLRNQRTKYDITSDAYPGGTVELSLDRVTVD
                     EAPGCGYTELELELLEGPEDILNDIVEMLNQQSDIFLARVGKYQRGLLASGRPPSLPS
                     AEPTPSPDKECRWLDLGLYNLRCELDDIRRYEPHAWESIHPEGVHQMRVATRRARAAL
                     RAFGDVLPPKAAKRLTRDIRWLTSSLGPLRDLDVQIAHIDAERERLDARDASAQALYR
                     KHLEKKRRRAHRRLVEALASDEYRKLIADFERLLEASASYHGPNRLMTIREAAHTQMK
                     PQLDRLLARGRNARKRPASRRLHRLRIALKRLRYQLEYLEGSYGKPMGNLTRAMEDVQ
                     DSLGNHQDAMVAVAQLERFRRKGGLKSGSRKSVKRLIAAEQENAARYRKRFRKDWRKF
                     EEAAAKMVKRL"
     gene            complement(3412..3834)
                     /locus_tag="AC16QF_14300"
     CDS             complement(3412..3834)
                     /locus_tag="AC16QF_14300"
                     /inference="COORDINATES: protein motif:HMM:NF019500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HIR6449781.1"
                     /translation="MDYLLATAVSVVLVALCISIHYEMLNLMARVVHRPERHRWIIMV
                     MILGLLFAHVVEIWLFGFGYLLAQEQLALGHITGTGDGLFDYIYYSAMVYTTVGFGDQ
                     VPGGAIRMISSTEALAGLSLITWSASFTFLQMQRLWRD"
     gene            3934..4009
                     /locus_tag="AC16QF_14305"
     tRNA            3934..4009
                     /locus_tag="AC16QF_14305"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3967..3969,aa:Val,seq:tac)
     gene            4027..4103
                     /locus_tag="AC16QF_14310"
     tRNA            4027..4103
                     /locus_tag="AC16QF_14310"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4061..4063,aa:Asp,seq:gtc)
BASE COUNT          706 a         1365 c         1436 g          777 t
ORIGIN      
        1 agggcagttc gagcgccatc tttccccctg atcgttggtc tcgggactca agaatgaccc
       61 ctagccggtt gggaggcaat tcgtccccgt cgaggagctt gcggcctggt agccacaatc
      121 ggctcaaggt ggcctacaat gccccccttc acgcgaggag aggagtagac ccgtggcaga
      181 accgtttcga ccagccccac cgaggaccgg gttcgggcag gccctgcgct cgaagcgcgc
      241 gagtaccatg gaagccgtcg aggacctgct ggcgcattgt gagtcgcttc tggacgtgac
      301 cgtgcaggcc gtggacggga tcgccgaagc ccagggggtg gatctggaga cgcgctttgc
      361 gagcgatcgc aaggacctgt accgccgcta tcttgcccac tgtcttgacg acaagatcct
      421 cacagaggac gagaacgccg atcttcagca cctgctcaat ctgctgcatc tgaatccgga
      481 cgacgtggtt cccgtgcacg acgagatggc ccgggaggtc tacggcaaag cgatccagga
      541 agtgctggcc gatcttgagg tggatgcgga cgaggaagcc tttctgcgac gcctgcgcgg
      601 cgatctgaag ctctccgacg acgtggcctc cgacctgctc gagcgcggcc gccgggacgc
      661 ccacgacgtg gcgctgcgcg aggcatccac gccggatcac gacttcctcg tctaccgcgc
      721 ccccgccggc gagttcaccg ggcggtcgga cgtgtctttc gaggcggccg tgaccgacgc
      781 gctctccaag gccgtgatcg caatccccat gctgcactgg ttcgaggtct ccaacatctc
      841 cggctatgta ggcgacggca aaccgagggg ctggcacgtg accgtgcggg gcgggatcga
      901 gccggagaag tagatgccgc gccgtcccgc ccgatggctc ctgtccgggt ggtgcgtcac
      961 cgccctgctg ggtctggccg cctgcgagcc tgggcatacg ccgtttccgg acggcgagat
     1021 cgtcgatctc tctcacacct acgacgagac caccgtgttc tggcccacgg ccgacggctt
     1081 tcagctggag gtggagtcgg ccggttacac ggataagggc tactactacg cggccaacaa
     1141 cttctccacc tccgagcacg gcgggacgca cctggacgcg cccatccact tcgccgaggg
     1201 cgcgcccgcc accgacgaga tcccgctgga gaggctggtg gggccggggg tagtgatcga
     1261 cgtgagcgct gcctgcacgg acgacgccga ctaccagatc gacgtggcgg acgtcacccg
     1321 atgggagcgt gctaacggcc agacggtgga cggcatgatc gtgctgttcc gcaccgggtt
     1381 cgccgagcgc tggccggatg tcgcgcgcta tctcggcacc gccgagcgcg gcgcggaggc
     1441 ggtggccaag ctgcattttc ccgggctgca tccggagctg gcggcgtggc tcgtggccga
     1501 gcgcgacgtg aaggcggtgg gtctggacac ggccagcatc gactacggtc agtcgaccct
     1561 ttacgagacc caccagatcc tgttcaaggc gggcatcccg gcgttcgaga acgtggccaa
     1621 cgtctcacgc cttccggcca gcggcttctc ggtggtggcg ttgcccatga agatcggtgg
     1681 cggcagcggc ggcccgttgc ggatcatcgc catcgtccca cccgcctgaa gggctacagc
     1741 cgcttgacca tcttcgccgc ggcttcctcg aatttgcgcc agtccttgcg gaagcgcttg
     1801 cggtaacgcg cggcgttttc ctgctctgcg gcgatcagcc gcttcacgga tttgcgcgag
     1861 ccggacttca accctccctt cctgcgaaat cgctcgagct gagcgacggc caccatggcg
     1921 tcctgatggt tgcccagaga atcctggacg tcctccatgg ccctggtgag gttgcccatg
     1981 ggtttgccgt acgaaccctc caggtactcc agctggtatc gcagccgctt cagggcgatc
     2041 cgcagccggt gcagccgccg cgaggcaggg cgcttgcggg cgttgcgtcc ccgggccagc
     2101 agccggtcca gctgcggctt catctgcgtg tgggcggcct cgcggatggt catcagccgg
     2161 ttggggccgt ggtagctggc ggaagcctcc agaagccgtt cgaagtctgc gatgagcttg
     2221 cgatactcgt cgctggccag cgcttccacc aggcgccggt gggcgcgtcg gcgcttcttt
     2281 tccaggtgct tccggtagag cgcctgggcc gatgcgtctc tggcgtcgag ccgttccctc
     2341 tccgcgtcga tgtgggcgat ctgcacgtcc agatcgcgca gcgggcccag gctggaggtg
     2401 agccagcgga tgtcccgggt gaggcgcttg gcggccttgg gcggcaggac gtcgccgaag
     2461 gcgcgcaggg cggcccgggc ccggcgagtg gccacccgca tctggtggac gccctccggg
     2521 tggatggatt cccaggcgtg gggctcgtag cgccggatgt cgtccagctc acagcgcagg
     2581 ttatacaggc cgaggtccag ccagcggcac tccttgtccg ggctgggcgt cggctctgcg
     2641 ctcggcagtg agggcgggcg tccgctggcg agcaggcccc gctggtactt gcccacccgg
     2701 gccaggaaga tgtccgactg ctggttcagc atctccacga tgtcgttcag gatgtcctcg
     2761 ggcccttcaa gtagctccag ctccagctcg gtgtacccgc agcccggggc ctcgtccacg
     2821 gtgacccggt ccagggacag ctccaccgtg ccgcccggat aggcgtcgct ggtgatgtcg
     2881 tatttcgtgc gctggttgcg caggtggaac agaggcttca gggaagcttc ctcggtgacg
     2941 atcacccata gctggtcggc cacctggccg gcgggcgggt ctccggggcg gttcaagggg
     3001 gcatcgagag actgctcgaa ctcttcgcgc ctgaacacgc cgtagtgccc ggtcttgatg
     3061 gccttcacgg ccagggtgcg cgtcttcttc tgctcgtcct cgcgcaggcg gtaggcccat
     3121 ccggcccgct gcagggccca gtccggcgtg tcgaagtagg tgtcgacgac caggcgctct
     3181 gagcgcagtc tggcccggta accggcccag ctcagcgtct gcagcacatg ctccagaacg
     3241 cgcctgggct ttctgcccgc agcgacgaga tatttggctt ccagctcgtt cacgggcggg
     3301 gaaataaatc aaagaagttg acagattatt acccgaatcg gcgcgccctg tcatcgatga
     3361 tccgatcgac ggtatcgccg gcggcgccga tcggtggcga tcgcccggtg ctcagtcgcg
     3421 ccagagcctc tgcatctgca ggaaggtgaa ggacgccgac caggtgatca gcgaaagccc
     3481 ggccagggcc tcggtgctgg agatcatgcg gatggcccct cccggcacct ggtcgccaaa
     3541 gcccacggtg gtgtagacca tggccgagta atagatgtag tcgaacagcc cgtcgccggt
     3601 gccggtgatg tgcccgagcg ccagctgttc ctgggccaac aggtagccga agccgaacag
     3661 ccagatctcc accacgtggg cgaacagcag ccccaggatc atgaccatga tgatccagcg
     3721 gtggcgttcg ggccggtgca cgaccctggc catgaggttc agcatctcgt agtggatgga
     3781 gatgcacaac gcgacgagga cgacggaaac cgcggtggcc aggaggtagt ccatgggcgg
     3841 attgtagcgc gactgcccaa ggcctcccac gtgccggcgg atccgattga cagccatagg
     3901 tcagggggta agatacgccc ccttgattcc ccagggtgtt tagctcagtt gggagagcga
     3961 cggccttaca agccgtaggt cgcaggttcg acccctgcaa cacccaccac tagtgaaggg
     4021 aacatcggag cggtagttca gttggttaga ataccggcct gtcacgccgg gggtcgcggg
     4081 ttcgagtccc gtccgctccg ccatctatat ctccaattat ctgaaatata agaagaaatt
     4141 ttcggacgaa aatttcagcc catacttctg cccatactcg aagaacacta gctcgggagg
     4201 ctggtggaag gactggtaac ctcgcttatt ggcgagtttc tcggggtgct tcaggattcg
     4261 gatgggttct ttgtttccga tctt
//
LOCUS       DBNHLM010000312         9672 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_646453, whole genome shotgun sequence.
ACCESSION   DBNHLM010000312 DBNHLM010000000
VERSION     DBNHLM010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9672
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_646453"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2601
                     /locus_tag="AC16QF_14315"
     CDS             <1..2601
                     /locus_tag="AC16QF_14315"
                     /inference="COORDINATES: protein motif:HMM:NF036578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HIR6449782.1"
                     /translation="LRCEYLSNPLGIDETRPRLSWWVDDDRPAELQTAYRILAASSPQ
                     RLAAEEGDLWDSGRVASHQTLNVEYHGGPLTTGQRVWWSVRSFDSDGLPSPWSEPAFF
                     EVGPCGQEDWSARWIGASLMGGAATSVQVPVFRRGFELPEAALAARLHVAALGVCAVE
                     INGQRVGRDELTPGWTDFRKRVRYRTYDVTDHLQTGENALGALLGDGWYCGHPGAGQR
                     QQYGDRPLLLAQLNITLASGATIAIGTDEAWKWRPSWLLQADVLGGESVDARQRIGDW
                     SSPGCADDGWDPAQVHDLPDLTVCASMHPPIRAQGVLEPVADAERRDDPVRPPRWIFD
                     FGQNLLGRVLVSVKAAAGSGVRVRYAESLGPTDDFGDRAGDGVDWYTASGVPGGETFE
                     PTFSVHGFRYVEVCGDLADDEPGVVAVAVNTDLDATATFESDHPLLDRLHANIVANQR
                     GCALDVPNAGFSLERRIPLTGEARAFLGTAAMNLDVAAFLAKWLTDLADAQLPDGGFP
                     SVAPVPPLVPALQQDGGPGWSDAFAACAWILYRHFGDRRVLERNYPALRTYVLGQAAA
                     WPDCLARPGSHPLGPADRGAVASLKATALFLQAVRLASRIAGVLGNLSDFEQFDGLAQ
                     SIRSAFRNRFVTHDGLVVGDCVTSYTLALHLGLLERGERRAAFEELVAKLEDGEPPAV
                     EPLAAPYLLGVLTRGGRVDLAYLVLLGSRPGSWLYPVLHGASSLWDGETESSRLARGS
                     VGEWLYTGLAGLDLDDDLSETNNAFRRIRIQPHPPLRDGIELAGNVPPMRRVKASLDT
                     VHGRYESAWEILEDGFVLRVQVPGNCSALVVMPDGTEHEVVAGRHEFKQDFLGETDEI
                     PILREIAEAS"
     gene            complement(2640..3671)
                     /gene="mnmH"
                     /locus_tag="AC16QF_14320"
     CDS             complement(2640..3671)
                     /gene="mnmH"
                     /locus_tag="AC16QF_14320"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HIR6449783.1"
                     /translation="MNGTDPETLLAEGARFIDVRAPSEFTRGAVPGAINLPILDDDER
                     HEVGSTYNNEGHNAAVALGYRLVSGAVRQARVAAWAEALDAYPEAWIYCWRGGQRSQI
                     AQEWLHEAGYEVPRVEGGFKALRQCCLEVLARAPHALPWLVLAGRTGSGKTVLIRERP
                     DSIDLEGQANHRGSAFGGRSCTQPSPISFENALAVAFLDLDSQRLIVEDESRTIGRLA
                     LPAAWHERMQTAPVVVVEADLSERVENIRREYVDEPLAEGVTPGDLQARYQGALERIS
                     RRLGGDRCRAVARALAEGFASGHHHAWIEQLLLWYYDPMYDYQLSKKQDRIVMQGDRD
                     AVREFLATP"
     gene            complement(3668..5374)
                     /locus_tag="AC16QF_14325"
     CDS             complement(3668..5374)
                     /locus_tag="AC16QF_14325"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6449784.1"
                     /translation="MKIIHFAAPSATASSPSRRATRLFPFGIQGAALIVVLAASMVGC
                     QSMPLDYAGLNDKIANGDQVAVGELRRAFLDLEDLPERMERLRDLELQAAQLFEDEPL
                     KLGSIGTAILDTYYGSLTGHFALSRFYEFVGSEEAAVPHRQWQARIREDMEASADGSR
                     ESPYPAVTDVEAQMYAVSLGLSPVGSIYQTGEAFPYTLLIQARPADGPLRNLYFDLGG
                     WYESMRRQFPDENEFSPFALIGFQARRGDTAAQVAVGAFLATQGRGDEAVDWLRAASR
                     TGNLLANSLLARIFWEEANKDGEDDARQAALDEVLENYLHAVALGSADAMYALGVLYM
                     NEHYGAENRDSGVPLLRQAAELEHSDAAMLLAHMHYTGEGVERDLDRATDYYVASAES
                     GNDFARRSYARFLLAEAREDPRALQWLEELAEDDDAEAMILLGNLHARGVGVKQSLRR
                     AVGWYKDAVDASGGDANIVNEVAWTLTVSDLEGLKRTRYALNIMEDLMTRDDEARRKP
                     EYLDTWAATYAANGDFIRAVAVQEEAVEAAEDDQYEGVREIIKTHLDAFKSGKTITET
                     AP"
     gene            5403..6056
                     /gene="msrA"
                     /locus_tag="AC16QF_14330"
     CDS             5403..6056
                     /gene="msrA"
                     /locus_tag="AC16QF_14330"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR6449785.1"
                     /translation="MAWEQKLRIPSESEALPGRAEEMPVPAAHHVNGAPLKEPFPEGL
                     AQAVFALGCFWGAERCFWNTPGVYSTAVGYAGGSTPNPTYEEVCSGLTGHTEVVLVVY
                     DPQKVSYAELLKVFWESHDPTQGMRQGNDVGTQYRSAIYTFDDEQAAAARASADEYQK
                     ALSAAGFSEITTEIRSAPAFYYAEPYHQQYLAKVPNGYCGLGGTGVGCNVAPLVKAV"
     gene            6101..6484
                     /locus_tag="AC16QF_14335"
     CDS             6101..6484
                     /locus_tag="AC16QF_14335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HIR6449786.1"
                     /translation="MGTSLDDARYFNLATFRKNGSAVETPVWFAPSAGEAPGVYYVFS
                     AGDAGKVKRLRRDQTARVAACDVKGKLQGEWLQARAELIEGADAQAALVALRLRYGWQ
                     MRLADLLSRLTGRFRRRAYIRVTLA"
     gene            6554..7942
                     /locus_tag="AC16QF_14340"
     CDS             6554..7942
                     /locus_tag="AC16QF_14340"
                     /inference="COORDINATES: protein motif:HMM:NF012242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR6449787.1"
                     /translation="MRVGVGIDFGTTNSAAATYDGERLQLVALEGADPIMPSATYIDR
                     ELQTLTGQAAINQYITDNTGRTVELVPEVIAETSAFVEHGDSENPAPVETVTQKIYGQ
                     PLIDSGLHGRLFRGTKRLLGDPRVRRLMVFDHPFRLVALITPILLRMRKALSRTVSGA
                     DYAHLGHPVNFEGRDEFRNQTALARLGEAYKYAGITQQRFYPEPIAAAVSYLFAHPEA
                     AGEHLLTLDFGGGTLDFCILRRAAGDFEVVTTHGIALGGDHIDQRLFRELLFPLLGKG
                     ETWRRRGMDREIETVFPFEDYQELLVNWAVTYTLNQNRYTTPVLECIGQGGPGARKFR
                     RLRELIQQNLSYVVFQTIKDFKADLSAREEAVLDISEIDVEVRLTRAGFESLIGDLLE
                     QAEQALDETVALAGLVHGDVDIVLCTGGSSLIPAVRRILEDRFPGRVVEHDPFTSVAA
                     GLAIANFHGMDV"
     gene            complement(7959..8567)
                     /locus_tag="AC16QF_14345"
     CDS             complement(7959..8567)
                     /locus_tag="AC16QF_14345"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6449788.1"
                     /translation="MHDLNLIRSRLSEHRAEEFAPEPDMREAAVAVILREVSSRPAEI
                     LFIKRAEKPGDPWSGHMAFPGGHRDPEDESLRAAAERETLEEIGLDLDSARYLGPLNQ
                     QRAVARGRPLNMLIAPHVFEIAGDPSFTINYEVAQVVWTPLAPLVSGALHDTETRPLA
                     GTPTVFNGYRLPQGHFVWGLTYRMVKSFFAAIDPGWEPPPEV"
     gene            8611..9009
                     /locus_tag="AC16QF_14350"
     CDS             8611..9009
                     /locus_tag="AC16QF_14350"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6449789.1"
                     /translation="MSLESRANRCFVCGPGNPIGLHVRFRLEEDVCLAEFTPGEEHVG
                     YDKVAHGGIIFSLLDDVMANWIWLRGEQCFTARADVRYRAQLPVGTPVRLEGRCLKRK
                     GRLAIMEGKVIRTDTEEVVAEASGSFMIPS"
     gene            complement(9068..9556)
                     /locus_tag="AC16QF_14355"
     CDS             complement(9068..9556)
                     /locus_tag="AC16QF_14355"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6449790.1"
                     /translation="MNAIKGLNHAAYRCRNSEETRQFYEDFLGLPLVDALPIGTTKTG
                     RAAQVLHTFFAMDDGSCVAFFEEPDAAFDFKDQRDFDLHLALTVDMETLHEMFERGKA
                     QGIETRGISDHGFIHSIYFRDPNGYVVELAAPVELRSGASADERRLRARQVLDEWEAR
                     RP"
BASE COUNT         1533 a         3099 c         3295 g         1745 t
ORIGIN      
        1 cttcgctgcg agtacctttc caatccgctt ggcattgacg agacccgacc ccgcctttcc
       61 tggtgggtgg acgacgaccg gcccgccgaa ctccagaccg cgtaccgtat tcttgccgcg
      121 agcagcccgc agcggctagc tgcggaagag ggcgatcttt gggactcggg tcgggtggcc
      181 agccatcaga ccctgaacgt ggagtaccac ggcggcccgc ttaccactgg tcagcgcgtg
      241 tggtggtcgg tgcgctcgtt cgacagcgac ggtctgccga gcccctggag cgaacccgct
      301 ttcttcgagg tcggcccctg cgggcaggag gactggagcg ctcgttggat cggcgcctcg
      361 ctcatgggcg gcgcggccac cagcgttcag gttcccgtct tcaggcgcgg tttcgagctt
      421 cccgaggcag cgctcgccgc tcggttgcac gtggccgccc tcggcgtctg tgccgtcgag
      481 atcaacgggc aacgtgtcgg acgtgacgag ctcacgccgg gctggacgga tttccgcaaa
      541 cgggttcgct accgcactta cgacgtgacc gaccatctcc agactgggga aaatgccctt
      601 ggcgcgctgc tgggggacgg ctggtactgc gggcaccccg gggccgggca acggcagcaa
      661 tacggtgacc gaccgctgct gctggcccag ctgaacatca ccctggcctc cggggcgacg
      721 atcgccatcg gcaccgacga agcctggaaa tggcgcccgt cctggttgct gcaggcggac
      781 gtgctcggcg gcgaaagcgt cgacgcccgt cagcgcatcg gggactggag ctcaccgggc
      841 tgcgcggatg acggctggga tccggcccag gtgcacgatc tgccggacct caccgtgtgc
      901 gccagcatgc acccccccat ccgtgcccag ggggtcctgg agcccgtggc cgatgctgag
      961 cgccgtgacg acccggttcg gccgccgcgc tggatattcg atttcgggca gaacctgctc
     1021 ggccgggtgc tggtcagcgt gaaggcggct gcgggctctg gcgtacgggt gcgatacgcg
     1081 gaaagcctgg gcccgacgga tgacttcggc gaccgggcag gagacggcgt cgattggtac
     1141 acggccagcg gcgtgcccgg cggagagacc ttcgaaccga ctttcagcgt acacggcttc
     1201 cggtatgtag aggtttgcgg cgatctggca gacgacgagc cgggagtggt tgcggtagcc
     1261 gtcaataccg acctggacgc cacggccacg ttcgagtcgg accatcccct gctggatcgc
     1321 ctgcacgcca acatcgttgc caaccagcgc ggctgcgccc tggacgtacc caatgcgggc
     1381 ttctccttgg aacgcaggat tccgctgacg ggggaggcgc gtgcctttct cggcaccgcg
     1441 gccatgaatc tcgacgtggc ggcctttctg gcgaagtggc tgacggatct ggcagatgcg
     1501 cagctgccgg atggggggtt ccccagcgtc gctcctgtgc ctccgctggt gcccgcgctg
     1561 cagcaggacg ggggcccggg ctggtcggac gcgtttgcgg catgtgcctg gattctctac
     1621 cggcatttcg gagatcggcg ggttctcgaa cgcaactatc cggcgctgcg gacctatgtg
     1681 cttgggcagg cggcggcctg gccggactgt ctggccaggc cgggcagcca tccgctcggg
     1741 cctgccgacc gaggtgccgt tgcctcgctg aaggcgacgg ctttgtttct tcaggcggtt
     1801 cggcttgcct cgcgcattgc cggggtgctg gggaatctga gcgacttcga gcagttcgac
     1861 ggcttagcgc agagtatccg ttccgccttc aggaaccgct tcgtgaccca cgacggcctg
     1921 gtggtcggcg actgcgtcac cagctatacc cttgctctgc atctagggct tctggaaagg
     1981 ggcgagcggc gcgcggcgtt cgaggagctg gtggcgaagc tggaagacgg cgagccgcct
     2041 gcagtggaac cgctggccgc accctatctc cttggggtgc tgacgcgggg cggccgcgtc
     2101 gatctggcct atctggtgct gctcggctcg cgcccgggca gctggctgta tcccgtgctg
     2161 cacggtgcaa gctcgctgtg ggatggggaa acggagtcga gccgccttgc tcggggctcg
     2221 gtaggcgagt ggttgtacac ggggcttgcc gggctcgatc tggacgatga tctatcggag
     2281 accaacaacg ccttccggcg catccggata caacctcacc cgccgttacg tgacgggata
     2341 gagctggccg gcaacgtccc cccgatgcgt cgggtcaagg cgtcgctgga taccgtgcac
     2401 gggcgctacg agtcggcctg ggagatcctc gaggacgggt tcgtgctccg cgtccaagtc
     2461 ccgggcaatt gctcggcgct ggtcgtcatg ccggacggga ccgagcacga ggtggtggcg
     2521 ggacgtcacg agttcaagca ggatttcctt ggcgagactg acgagatccc cattctccgg
     2581 gagattgccg aggcgtcttg attccctgcg gcggggttgg gccgccgtgg ggcagccggt
     2641 cagggtgtgg cgagaaactc ccggaccgcg tcgcggtcgc cctgcatgac gatgcggtcc
     2701 tgcttcttgc tcagctggta gtcgtacatg gggtcgtagt accacagcag cagctgctcg
     2761 atccaggcgt ggtggtggcc ggaggcgaag ccctccgcga gggcgcgcgc cacggcccga
     2821 cagcggtcgc cgccgaggcg gcggctgatg cgctcaagcg ctccctggta gcgggcctgc
     2881 aggtcgccgg gcgtcacgcc ctcggcaagc ggttcgtcaa cgtactcgcg gcggatgttc
     2941 tccacccgct cggacaggtc ggcttccacc accaccacgg gcgcggtctg catgcgctca
     3001 tgccaggcgg ctggcagggc gaggcggccg atggtccggc tttcgtcctc cacgatcaat
     3061 cgctgggagt cgagatcgag gaaggccacc gccagagcgt tctcgaagct gatcggggat
     3121 ggttgcgtgc acgatctgcc gccgaacgcg gagccgcgat ggttggcttg gccttccaga
     3181 tcgatgctgt ccggccgctc acgaatcagc acggttttgc ccgagccggt ccgcccggcg
     3241 aggaccagcc agggcagcgc gtgcggcgcc ctggccagca cctccaggca gcattgccgc
     3301 agagctttga agccgccttc gacccggggg acttcgtagc ccgcttcgtg cagccactcc
     3361 tgtgcgatct gggagcgctg gcccccacgc cagcagtaga tccacgcctc ggggtacgcg
     3421 tctagggcct cagcccaggc ggctacgcgg gcttgcctca cggcgccgct caccaggcgg
     3481 taccccaggg cgacggcagc gttgtgaccc tcgttgttgt aggtggagcc gacttcgtgg
     3541 cgctcgtcgt catccaggat cggcaggttg atggcgccgg gtacggcgcc ccgggtgaac
     3601 tcggagggcg ccctgacgtc gatgaagcgc gcgccttcag ccagcagcgt ttccggatcg
     3661 gttccgttca cggcgcggtt tcggtgatcg tctttccgct cttgaaggca tccagatggg
     3721 tcttgatgat ctcccgcacg ccctcgtatt ggtcgtcctc tgccgcctcg acggcttcct
     3781 cctgaaccgc cacggcgcgg atgaaatcgc cgttggccgc ataggttgcc gcccaggtgt
     3841 ccaggtactc cggcttgcgg cgcgcctcgt cgtcgcgggt catgagatct tccatgatgt
     3901 tgagcgcgta gcgtgttcgc ttcaggccct ccaggtcgct gaccgtcagc gtccatgcca
     3961 cctcgttcac gatgttcgca tcgcccccgg acgcgtccac ggcgtccttg taccatccga
     4021 ccgcgcgccg caggctctgt tttaccccga cgccgcgtgc gtggaggttg cccagcagga
     4081 tcatcgcctc cgcatcgtca tcctcagcaa gttcctccag ccactgcagg gccctgggat
     4141 cctcgcgcgc ctcggccagc aggaaacgcg cgtagctgcg acgagcgaag tcgttgccgc
     4201 tctcggctga agcgacgtag tagtccgtcg cgcggtccag atcgcgctcg acgccctcgc
     4261 cggtgtagtg catgtgcgcg agcagcatgg cggcgtcgct gtgttccagc tccgctgcct
     4321 ggcgcagcag cggcaccccg gagtcacggt tctcggctcc gtagtgctcg ttcatgtaga
     4381 ggacgcccag cgcgtacatg gcgtcggccg agccgagcgc gacggcgtgc aggtagtttt
     4441 ccagcacctc gtccagcgcg gcctgccggg catcgtcttc gccgtccttg ttcgcctcct
     4501 cccagaagat tcgggcgaga agggagttgg cgagaaggtt tccggtacgc gaggccgctc
     4561 gcagccagtc cacggcttcg tcgcctctgc cctgggtggc gagaaacgcg ccgacggcga
     4621 cctgggctgc ggtatcgccg cgccgggcct gaaagccgat cagcgcgaac ggcgaaaatt
     4681 cgttttcgtc ggggaactgc cggcgcatgg actcgtacca tccgcccaga tcgaaataca
     4741 ggttgcgcag aggcccatcg gccggccgcg cctggatgag cagcgtgtag gggaacgcct
     4801 cgcccgtctg gtagatggag cccaccgggc tcaagcccag gctgacggca tacatctggg
     4861 cttccacgtc ggtaaccgcc gggtaaggcg attcccggct gccatccgcg gacgcctcca
     4921 tgtcctcgcg aatgcgcgcc tgccactgcc ggtggggtac cgcggcctct tcggagccca
     4981 cgaactcgta gaaccgcgaa agcgcgaagt gacccgtcag gctgccgtag taggtatcca
     5041 gaatggcggt gccgatggat cccagcttca gaggttcgtc ctcgaacagc tgcgccgcct
     5101 gcagctccag atcgcgaaga cgctccatcc gttccggcag atcctccaga tcgagaaacg
     5161 ctctgcgcag ctcgcccacg gcgacctgat ccccgtttgc aatcttgtca ttgaggccgg
     5221 cgtagtcgag gggcatgctc tggcagccga ccatcgatgc tgccagcacg acgatcagag
     5281 ccgccccctg aatcccgaag gggaagaggc gagtggctcg gcggctgggc gaactggcag
     5341 ttgcggaggg ggcggcgaaa tgtatgattt tcatcttcct tcaatgttac aggcaattgg
     5401 acatggcttg ggagcaaaaa ctccgtatcc ccagtgaatc cgaggctctg ccgggacgag
     5461 ccgaggagat gcccgtgccg gcggcacacc acgtcaacgg cgcccccctc aaggaaccgt
     5521 ttcccgaggg cctcgctcag gccgtgttcg ctcttggttg tttctggggc gcggagcggt
     5581 gtttctggaa cacgcccggg gtgtactcca cggccgtcgg ctatgccggc ggcagcacgc
     5641 cgaacccaac ctacgaggaa gtctgctccg ggctgacggg gcacaccgaa gtcgtgctcg
     5701 tcgtatacga tcctcagaag gtttcctacg ccgagctgct caaggtcttc tgggagtcgc
     5761 acgaccccac ccagggcatg cgccagggca atgacgtggg cacccagtac cgctcggcga
     5821 tctacacgtt cgacgacgag caggcggccg ccgcgcgggc gtccgccgac gaatatcaga
     5881 aggcgctttc tgctgccgga ttttccgaga tcacgacgga gataaggtct gccccggcgt
     5941 tctactatgc cgagccgtac catcagcagt accttgcgaa ggttcccaac ggctattgcg
     6001 gtcttggagg gaccggagtc ggctgtaacg tggcgcccct cgtgaaggcg gtttgaattc
     6061 gcgataaact ccgccgcttg cttcgcggcg gagattgtcg gtgggcacct cgctcgacga
     6121 cgcacgctac ttcaatctgg ctaccttcag gaagaacggc tcggcggtcg agacgccggt
     6181 gtggtttgct ccctcggcgg gcgaagcgcc gggcgtttac tacgtcttct cggcgggtga
     6241 tgccggaaag gtcaaacgtt tgcgccgtga ccagaccgcg cgggttgctg cctgtgacgt
     6301 caagggcaag ctgcaggggg agtggctgca ggcacgcgcg gagctgatcg agggcgcgga
     6361 cgcccaggcg gcgctcgtgg cgctgcgcct ccggtacggg tggcagatgc gacttgccga
     6421 tctcctctcg cgattgacgg ggcggttccg ccggcgtgct tacatccggg tgactcttgc
     6481 gtgattgcgc cggccggcaa ggtttccggg acgaggcgtt ggagatgaat tggcaacgag
     6541 agggcggcaa ctgatgcgcg tgggggtcgg cattgatttc ggcacgacca actcggcggc
     6601 ggccacctac gacggcgagc ggctgcagct cgttgcgctg gagggcgccg atccgatcat
     6661 gccctccgcc acctacatcg accgtgagct gcagacgctt accggtcagg cggccatcaa
     6721 tcagtacatc accgacaata ccggccgaac cgtggagctg gttccagagg tgatcgcaga
     6781 gaccagcgcc ttcgtggagc atggggattc ggagaacccc gcgccggtgg aaaccgtcac
     6841 ccagaaaatc tacgggcagc cgctgatcga cagcggcctg cacgggcggc tgttccgggg
     6901 gaccaagcgg ctcctgggcg atccccgggt gaggcgcctg atggttttcg atcatccttt
     6961 ccgcctggtt gccctcatca cgccgatact gcttcgcatg cgtaaggctc tctcccggac
     7021 ggtgagcggg gccgactacg ctcacctggg ccatccggtg aacttcgaag gccgggacga
     7081 gttccgcaac cagactgctc tggcgcgact gggggaggct tacaagtatg cgggcatcac
     7141 gcagcagcgc ttttatccgg agcccatcgc cgctgccgtg agttatctct ttgcccatcc
     7201 cgaggctgcc ggtgagcatc tgctcaccct ggatttcggc ggcggcacgc tggatttctg
     7261 catactgcgg cgggctgccg gcgatttcga ggtggtcacc acccatggta tcgcgctggg
     7321 cggcgaccac attgatcagc gcctgtttcg cgagctgctc ttcccgctgc tcggcaaagg
     7381 cgagacgtgg cgccggcggg gtatggaccg ggagatcgaa accgtcttcc ccttcgagga
     7441 ttaccaggag ctcctggtca actgggcggt gacctacacg ctcaaccaga accgctacac
     7501 cacgccggtc ttggagtgca tagggcaggg cggtccgggc gcgcgcaagt tccgccggct
     7561 tcgggagctg atccagcaga acttgagcta cgtggtgttt cagaccatca aggacttcaa
     7621 agcagatctt tcggctcggg aagaagcggt gctggacata tccgagatcg acgtggaggt
     7681 gcgtttgacc cgtgcgggtt tcgaatcgct gatcggcgac ctcctggaac aggcggagca
     7741 agcgctggac gaaacggtag cgctcgccgg gctcgtccat ggggacgtgg acatcgtgct
     7801 gtgcactggc ggctccagcc tgattcccgc ggtgcggcgc attctggagg atcgatttcc
     7861 cgggcgggtc gtggagcacg atccgttcac cagcgtggcc gcaggcctgg ccatcgccaa
     7921 cttccacggc atggacgttt aggcgtcagc ggcgctcgct agacctccgg cggaggttcc
     7981 cagcccgggt cgatggcggc gaagaacgac ttcaccatcc ggtaggtgag accccagaca
     8041 aagtgtccct ggggcaggcg gtagccgttg aacaccgtcg gcgtgcctgc cagcggacgg
     8101 gtctcggtat cgtgaagcgc ccctgatacg agcggtgcca gtggggtcca gaccacctgt
     8161 gccacctcgt agttgatggt gaacgaaggg tccccggcga tctcgaaaac gtggggcgcg
     8221 atgagcatgt tgagggggcg gccgcgggcc acggcgcgct gctggttcag cggacccagg
     8281 tagcgagccg aatcgaggtc cagcccgatc tcctccagag tctcccgttc tgcggccgct
     8341 ctgagactct cgtcctccgg gtcccggtgc ccgcctggaa aagccatgtg ccccgaccag
     8401 gggtctccgg gcttctccgc ccgcttgatg aacaggatct cggccggccg gctggagacc
     8461 tcacgcagga tgacggccac ggcggcctcc cgcatgtcgg gctccggcgc aaactcttcc
     8521 gcgcggtgct cggacaggcg ggatcgaatt aaattcaggt cgtgcacttg ggctatcata
     8581 ccggctgggt cgggcaactc taaattcagc atgtcattag aaagtcgggc taaccgctgc
     8641 ttcgtctgcg ggcccggaaa ccccatcggt ctccacgtgc gctttcgcct cgaggaggac
     8701 gtctgcctgg cggaattcac gccgggcgaa gagcacgtcg gctacgacaa ggtagcacac
     8761 ggaggaatca tcttcagcct gctggacgac gtcatggcga actggatctg gctgcgcggc
     8821 gagcagtgct tcactgcccg ggcggacgtc cgctaccggg cgcagctgcc ggtgggcacg
     8881 ccggtgcgtc tggaaggccg ctgcctgaag cgcaagggcc gcctcgcgat catggaaggc
     8941 aaagtgattc gcacggacac cgaggaggtg gtcgcggagg ccagcgggag cttcatgatc
     9001 ccgtcctgac gcggcgccgg cctgcagcgg tctcgagccg cgtgaacggc aagcggcatc
     9061 gggaaagcta gggccgtcgt gcctcccact cgtccagaac ctgccgggcg cgcagccgcc
     9121 gctcatccgc gctagcgccg cttcgcagct ccacgggtgc cgccagctcg accacgtagc
     9181 cgttcggatc gcggaagtag atcgagtgaa tgaacccgtg atctgagatg ccgcgcgtct
     9241 cgatgccctg cgcctttccc cgctcgaaca tctcgtggag ggtctccatg tcgacggtca
     9301 gtgccagatg cagatcgaag tcgcgctgat ccttgaagtc gaacgccgcg tccggctcct
     9361 cgaaaaaggc gacgcaggag ccgtcgtcca tggcgaagaa cgtgtgcagc acctgggcgg
     9421 cgcggccggt cttggtagtg ccgatgggca gggcgtcgac caggggcaat cccaggaaat
     9481 cctcgtagaa ctgccgggtc tcctcagagt tgcggcatcg ataggcggcg tggttcaagc
     9541 ccttgatggc gttcattggc tgctccgtcg tcggttcgaa ggatagtttc gctcgagcca
     9601 gtccagcatc aggcgattgg tctcgccggg cagttcctgc tggatccagt ggccgcagtc
     9661 gagcacgtgg gt
//
LOCUS       DBNHLM010000313         5294 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_406800, whole genome shotgun sequence.
ACCESSION   DBNHLM010000313 DBNHLM010000000
VERSION     DBNHLM010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_406800"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="AC16QF_14360"
     CDS             complement(<1..359)
                     /locus_tag="AC16QF_14360"
                     /inference="COORDINATES: protein motif:HMM:NF013962.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR6449791.1"
                     /translation="MADYVLTFSCPDRPGLVHAVSSWLVDLNGNILDSDQFRDDTTER
                     FFMRVHFEAPDDLPALNQTFIPTAGAMNMDWQIWPTARRPRVLMLVSKIDHCLADLLY
                     RRRIGELKIDMPLIASN"
     gene            complement(653..2059)
                     /locus_tag="AC16QF_14365"
     CDS             complement(653..2059)
                     /locus_tag="AC16QF_14365"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6449792.1"
                     /translation="MAEPAKPERLKQSTLMLFGLPEYAVYLGSIPVGLYLPFVYSKDL
                     GLELVDVGLILMLARISDVITDPLIGYLSDRTGGRFGRRKPWLAAGALLMMVSAFQLF
                     NPNALSAAPVDNWHLLSWSVLLWLGWTMINIPYYAWGAELSDDYNERTRITGWRQAFG
                     FLGNVSVLAVPVIAGEITGYGSMPREGLTIIGSMALVALPTLIAVTLWRVPEREAYAK
                     PKASVLTHARAMSRNGSFILLFLGFMLMSLGTGWGSATFMLFATYVVGAEGQTQAILL
                     GYFGANVIALPLWVALAQRIGKKTTWLIGGVLFVIVTPCFLLLEHGDLWWFFLCLALY
                     GVAGGNFAALSMSMKADVIEIAARRSGENMAGSYIAVWSLGQKMVAALALGLALPFLQ
                     LLGFDPAGGNDAEQIRALALVYVVPPWLFYALAVVVIWRYPITASRLQRIRAAFERRD
                     ARRQRKGPAGFAEQAEPT"
     gene            2211..3503
                     /locus_tag="AC16QF_14370"
     CDS             2211..3503
                     /locus_tag="AC16QF_14370"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6449793.1"
                     /translation="MSQGQKTPYEPVVPEKTDDIDLLDVGLQNCPYHAYRVLRDDAPV
                     WQDPRTGFFVITRFEDLRQVLLDTEHFSNGANSNARDKLDSDRAMRMRELYETKGWVP
                     APTLAGRDDPNHKQMRSMFDEAFRPARINSMEPLVEGTARKLIDAFVDDGHCDWVKQF
                     AVPMPLIIIGVQMGAKEEDIWRIKAWTDAWVQRLGMMQTEEEERWSVEMEIEAQNYFQ
                     PIFERLRKEPDDSLISDLVNRVIPEWGRPLNDNELHAEMMADTFVGGSETSTNALSYG
                     VKLLIENPDSWDRLKSDPDKYLRTFCEEVLRLEGPVQGLFRRAAKDIELHGVQIPEGS
                     MINIRYAAANRDEREFENPEKLDLDRKKPGRHLAFGSGIHHCLGAPLARRELYWGFKV
                     LVERIDEMWFAPGKNDFTVAPNFSLRALKELHIEFNKA"
     gene            3514..4284
                     /locus_tag="AC16QF_14375"
     CDS             3514..4284
                     /locus_tag="AC16QF_14375"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6449794.1"
                     /translation="MNMHDKFRLDGQVAVVTGGGRGIGRGIALALADCGADVVVVARR
                     QADVEEVAEEIRERGRRGLGVSADVMDFTAIPGVLDRVVEEFGRLDIMVNNAGGNLDR
                     KTYSLPDIPIEKFDEQLTLNMKTKWWGAQQAAKRMENGGRIINIISVAAHRAAPGFGV
                     YSAANMGMISMTRTLAVELAPKKITVNCIAPGIIVTEMLKETMHISEEEAHQTFDAQI
                     PLGRTGTPEDCAAAAVFFASPAAEWTTGQFLDVAGGPA"
     gene            complement(4371..5021)
                     /locus_tag="AC16QF_14380"
     CDS             complement(4371..5021)
                     /locus_tag="AC16QF_14380"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6449795.1"
                     /translation="MPATRSLTANQAQRRAKILQATRDLVTEHGYDGMIMRDVAALAD
                     VSATTLYNLYNTKDELLLEALRDRIEASTVQARQHAPEPGYEYLLAHVRSVCRQTRRS
                     PAYVNAIAQALFRSTPGDPLAEVLLAGLQEDVERSLQAMSDAGHLHPQARINALARAL
                     TGSFWSNFLLWNKGRLALKDLETAQLSGYLSLLIPTTVGSANEALRSRLKALSGLS"
     gene            complement(5035..>5294)
                     /locus_tag="AC16QF_14385"
     CDS             complement(5035..>5294)
                     /locus_tag="AC16QF_14385"
                     /inference="COORDINATES: protein motif:HMM:NF024247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6449796.1"
                     /translation="SCIEVCPVDCIYFDEEDDRMCYIQPDECIDCAVCESACPVGAIF
                     KDEDVPKEVEEFTAINLLWFEDKEAARAKIDAFAAGNSSIT"
BASE COUNT         1062 a         1571 c         1749 g          912 t
ORIGIN      
        1 tgattcgagg cgatcaaggg catatcgatc ttcagttccc cgattcgcct gcggtacagg
       61 agatcggcca ggcagtggtc tatcttggag accagcatga gcactcgggg ccgtcgcgcg
      121 gttggccaga tctgccaatc catgttcatg gcaccggcag tcgggatgaa cgtctgattc
      181 aaggcgggaa ggtcgtcggg cgcctcgaaa tgcacccgca tgaaaaagcg ttccgtggtg
      241 tcgtcacgga actgatcact gtcgagtatg ttcccgttca ggtccaccag ccagctcgat
      301 accgcatgaa ccagcccggg acgatcggga catgagaaag tgagaacgta atcagccaag
      361 cacgaacacg ccgcaattcc agcggcgtgc atcgtacctg cgatcagcct ccaaagcgaa
      421 cgcgaatggt ccttccggca atggcaagcg ccatgccgcc cgcccttacg gcgatgttca
      481 gaccttgatc agccccgctg cgatggcgat gagcgtgatc gtgatcccgg taacggccac
      541 gaggccaagt gccatgctga tgtgctgtgc ggtctcaacc atgatccatc tccacggttt
      601 gattgatcat ggcttttata ggacgggcag cgaacgcgga gaatcagggg atctacgtag
      661 gctccgcctg ctcggcgaaa ccagccgggc ccttgcgctg ccgacgcgcg tcgcggcgtt
      721 cgaaagccgc ccggattcgt tgcaggcggg aggccgtgat cgggtagcgc catatcacca
      781 ccaccgccaa cgcgtaaaac agccagggag gaaccacgta gacgagcgcg agggccctga
      841 tctgctcggc atcgttgccg ccggcagggt cgaaaccgag cagttgcaga aagggtagcg
      901 ccagacccag agccagggcg gcgaccatct tctggccgag ggaccagacc gcgatgtagg
      961 aacccgccat gttctcgccg gaccggcgcg cggcgatctc gatcacgtct gccttcatgg
     1021 acatcgacag cgcggcgaaa ttgcccccgg ccaccccgta gagtgccagg caaaggaaga
     1081 accaccagag atcgccgtgc tccagcagca ggaagcaggg ggtgacgatg acgaagagca
     1141 cccctccgat cagccaggtg gttttcttgc cgattcgctg ggcgagggcc acccacagag
     1201 gcagggcgat aacgttcgcg ccgaagtaac cgagcagaat ggcctgagtc tgtccctcgg
     1261 ccccgacgac gtaggtggcg aacaacatga acgtcgccga tccccagccg gtaccgaggg
     1321 acatgagcat gaatcccagg aacagaagga tgaaggagcc gttgcgtgac atcgcccgcg
     1381 cgtgggtgag caccgatgcc ttcggcttcg cgtaggcctc gcgttcgggg acgcgccaga
     1441 gggtcacggc gatcaacgtg ggcagcgcca cgagcgccat ggatccgatg atcgtcaggc
     1501 cctctctggg catgctgccg tatccagtga tctcgccggc aatgacgggc actgccagga
     1561 cgctcacgtt cccgaggaag ccgaacgcct gccgccatcc ggtgatgcgg gtgcgttcgt
     1621 tgtagtcgtc ggagagctcg gcgccccagg cgtagtaggg gatgttgatc atggtccagc
     1681 caagccagag cagcacgctc cagctcaaca ggtgccagtt gtccaccggt gctgcgctga
     1741 gcgcgttcgg gttgaagagc tggaatgccg agaccatcat caggagcgcg cccgccgcga
     1801 gccagggctt gcggcggccg aagcggcctc ctgtgcggtc gctgagatag ccgatcaacg
     1861 ggtcggtgat cacatcggag atgcgcgcca gcatcagaat gaggcccacg tcgaccagct
     1921 ccaggccgag gtctttggag tagacgaaag gcagatacag cccgaccggg attgatccga
     1981 ggtagacggc gtattccggg agcccgaaca acatcagcgt ggactgcttc aggcgctctg
     2041 gttttgcggg ctcggccacc ggtggctcca cgaagctgac gaaccaaggc tagggggtga
     2101 tgcttcgtgg ggtcaacaaa atttctggtg agcgctacaa taaattgccg ttctggatgg
     2161 ccaggacgga caatggcccc atggggccgc gcatcacagg ggaggacgcc gtgtcacaag
     2221 ggcagaagac accatacgag ccggtagttc cagagaagac cgacgacatc gacctgctgg
     2281 atgtcggtct gcagaattgt ccgtaccacg cctatcgggt cttgagagac gacgcccccg
     2341 tgtggcagga cccgaggacg ggctttttcg tgattacccg attcgaggat ctgcgacagg
     2401 ttctgctgga taccgagcac ttctcgaacg gggcaaacag caacgcgcgc gacaagctcg
     2461 attccgatcg ggccatgcgg atgcgcgagc tctacgaaac caagggatgg gttccggcgc
     2521 cgaccctggc cggtcgcgac gatccgaacc acaagcagat gcggtccatg ttcgacgaag
     2581 cgttcaggcc ggcgcggata aactccatgg aaccgcttgt agagggcacg gcgcgcaagc
     2641 tcatcgacgc ctttgtcgac gatggacatt gcgactgggt caagcagttc gccgtcccca
     2701 tgccgctgat catcatcggc gtgcagatgg gggccaagga ggaggacatc tggcggatca
     2761 aggcgtggac ggatgcctgg gtccagcgcc tcggaatgat gcagaccgag gaagaggagc
     2821 gctggtccgt ggaaatggag atcgaggcgc agaactactt tcagccgatc ttcgagcgac
     2881 ttcgcaagga gccggacgat tctctcatct cggatctcgt gaaccgtgtc attcccgagt
     2941 ggggcaggcc gctcaacgac aatgagctgc acgccgagat gatggccgat accttcgtcg
     3001 gaggctcgga gacctccacg aacgcgctgt cctacggagt gaagctgctc atcgagaacc
     3061 ccgattcctg ggaccggctg aagtcggatc cggataaata ccttcgtacg ttctgcgagg
     3121 aggtgctccg gctcgaaggc ccggtgcagg ggttgttccg gagagcagcc aaagacatag
     3181 agctgcacgg cgtgcagata ccggaaggat caatgatcaa catcagatac gcggctgcga
     3241 accgggacga gcgggaattc gagaatccgg agaagctcga tctcgatcgc aagaagcccg
     3301 ggcgccacct cgcgttcggt tccggcatac accactgcct cggggctccg ctggcgcggc
     3361 gcgagctcta ctggggtttc aaggtgctcg tggagcggat agacgagatg tggtttgccc
     3421 ccggtaagaa cgacttcacc gttgcaccga atttctctct gcgcgctctc aaggagctgc
     3481 acatcgagtt caacaaggcc taggaggcgc gaaatgaaca tgcacgacaa gttccgactg
     3541 gatggtcagg tagccgtggt caccggtggt ggacgcggga tcggacgggg aatcgcgctg
     3601 gcactggccg actgcggagc ggatgtggtg gtggtcgccc gccgtcaggc agacgtcgaa
     3661 gaggtggcgg aggagattcg cgagcggggc cggcgcgggc tgggcgtctc cgcggacgtc
     3721 atggacttca cggccattcc cggggtcctg gaccgggtgg tggaagagtt cggccgtctg
     3781 gacatcatgg tgaacaacgc gggggggaat ctggatcgga aaacgtactc cctgcccgac
     3841 atacccatag agaagttcga cgagcagctc accctgaaca tgaagaccaa gtggtggggt
     3901 gctcagcagg cggccaagcg aatggagaac ggtggccgca tcatcaacat catttccgtg
     3961 gccgcccatc gagcggcgcc ggggttcggc gtctactctg cggccaatat ggggatgatc
     4021 agcatgaccc ggacgctggc ggtggagctt gcgcccaaga aaatcaccgt gaactgcatc
     4081 gcgcccggca tcatcgtcac cgagatgctc aaggaaacca tgcacatttc cgaggaggaa
     4141 gcccaccaga ctttcgatgc gcagattccg ctcgggcgga cgggcactcc ggaggactgc
     4201 gcagccgctg ccgtgttctt tgcgtccccg gcggcggagt ggaccaccgg ccagttccta
     4261 gacgtcgcgg gggggcccgc ctagggccga cctgggctag ccgaccgcgt ctggcagacc
     4321 gcgtctagcc gaccgcgtct agccgaccgc gtctagccga ccgcttgggt tcagctcaga
     4381 ccgctcagag ccttcaggcg actgcgcaag gcttcgttgg cggagccgac ggtggtcggg
     4441 atcagcaggg aaaggtagcc gctcagctgg gcggtttcca ggtccttgag cgcgagccgg
     4501 cccttgttcc acagcagaaa gttggaccag aaggaacccg tcagggctcg ggcgagcgcg
     4561 ttgatgcgcg cttgggggtg caggtgcccg gcgtcgctca tcgcttgtag cgaccgctcc
     4621 acgtcctcct gcagccccgc cagcagcacc tcggcaaggg ggtctcccgg agtggagcga
     4681 aacagcgcct gggcgatcgc gttcacgtag gcaggggagc ggcgggtctg ccggcagacg
     4741 ctgcggacgt gggccaggag gtactcgtag cccggttcgg gagcgtgctg acgggcctga
     4801 acggtgcttg cttcgatcct gtcgcgcagg gcctccaaaa gcagctcgtc cttggtgttg
     4861 tagaggttgt aaagggtcgt agccgagacg tctgcgaggg cggccacgtc ccgcatgatc
     4921 atgccatcgt agccgtgctc ggttaccaga tcccgggtag cctgcaggat cttcgcccgc
     4981 cgctgggcct gattggccgt caacgagcgt gttgcaggca cgggctcgct ccgttcaggt
     5041 gatggacgag ttaccggccg cgaaggcatc gatctttgcc cgcgcggctt ctttgtcctc
     5101 gaaccagaga aggttgatgg ccgtgaactc ctcgacctcc ttcggcacgt cctcgtcctt
     5161 gaatatggct cctaccggac aagccgactc gcacaccgcg cagtctatgc actcgtcggg
     5221 ctggatgtag cacattcgat cgtcttcctc gtcgaagtaa atgcagtcca ccgggcacac
     5281 ttcaatgcag gatt
//
LOCUS       DBNHLM010000314         9861 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_586347, whole genome shotgun sequence.
ACCESSION   DBNHLM010000314 DBNHLM010000000
VERSION     DBNHLM010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9861)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9861)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9861
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_586347"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..952
                     /gene="fabD"
                     /locus_tag="AC16QF_14390"
     CDS             50..952
                     /gene="fabD"
                     /locus_tag="AC16QF_14390"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007945732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR6449797.1"
                     /translation="MFPGQGSQTVGMLLDISAEYPQVIECFQRAGDSIGRPLWEITTS
                     GPDTELNSTEITQPALLTASVALWEIWQTSGGEAPAMLSGHSLGEYSALVCAGSLSLE
                     DGVRLVHERGKLMQKAVPHGKGAMAAVLGLDDDVVAACCEAVDGVVSPANFNAPGQVV
                     IAGATAAVESAVEACREAGARRAVLLPVSGPFHCELMRPVQNAFADVLGRVDLVMPSI
                     PIVHNVDGEVAPDLDALRSKLMAQIAEPVLWTRCAQNMVAAGVTRLVECGPGKVLSGL
                     VKRIDKSVQAESLGTLDGLRAALG"
     gene            953..1684
                     /locus_tag="AC16QF_14395"
     CDS             953..1684
                     /locus_tag="AC16QF_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HIR6449798.1"
                     /translation="MAAERKVALVTGASRGIGAAIAQRLSLDHHVVGTATSEQGALAI
                     SESLGDAGEGLIMRVQDDASVSAALDRLAQGCGMPAVVVNNAGITRDNLLLRMKPEEW
                     QEVMDTNLGALYRVVKPCLRSMMKSRWGRIVNLSSVVARMGNPGQSNYVASKAGIEGF
                     TRSLAIEVASRGITVNAVAPGFIETDMTSQLSQDQVEEMLARVPAGRMGSADEVAEAV
                     AYLVSDAAGYITGTTLAINGGLHMT"
     gene            1824..2057
                     /gene="acpP"
                     /locus_tag="AC16QF_14400"
     CDS             1824..2057
                     /gene="acpP"
                     /locus_tag="AC16QF_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR6449799.1"
                     /translation="MSSVEERVKKLICEQLGVKEDEVKDEASFVEDLGADSLDTVELV
                     MALEEEFETEIPDEEAEKITTVKEAIDYILAHQ"
     gene            2144..3379
                     /gene="fabF"
                     /locus_tag="AC16QF_14405"
     CDS             2144..3379
                     /gene="fabF"
                     /locus_tag="AC16QF_14405"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR6449800.1"
                     /translation="MTRRRVVVTGIGLLTPIGNDVDSAWSNALAGKSGAGPIEQFDAT
                     DFSVRICAPVKGFDATQYVDRKEVRRNDPFIVYGMAAGVQAIEDAGLDAHPADGHRYG
                     LAIGSGIGGLGTIEDTHGSLVAGGPRKVSPFFIPASVINMISGNLSIRYGFTGPNVAV
                     VTACTTGTHNIGFGARMIQSGDADVMVVGGAEYATTPVAVAAFSMMKALSTRNDEPEL
                     ASRPWDRDRDGFLLGEGAGVLVLEEYERAKARDAKIYAEVVGFGMSADAFHITSPAED
                     GAGAAACMQNALADAGLRSDQIDYINAHGTSTPLGDVAETVAIKRVFGDDTRVAVNST
                     KSMIGHLLGAAGSVEAIFTILAIRDQVSPPTINLDNPGEGCDLDYVPNVARQMQIRAG
                     LSNSFGFGGTNGTLIFASV"
     gene            3409..4395
                     /gene="mltG"
                     /locus_tag="AC16QF_14410"
     CDS             3409..4395
                     /gene="mltG"
                     /locus_tag="AC16QF_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR6449801.1"
                     /translation="MRLFLVSFAALILLSGSAAFYVVRWNDAPLALTEPVVVVLEAGE
                     PFSKFAARISASGYLTHPRLWSRLARLRGLARQAKAGEYALQPGDTPERLLQRIVRGE
                     VVTYEVRLLEGWTVMQVLLALQENQVLEHELVGANVTTLLEALGLPGGHAEGLFYPDT
                     YQFVRGSSDRDILRRAYDRMQMVLEGAWDQRASGLPYRTPYEALIVASLVEKETGRDE
                     DREHISQVFVSRLNANMRLQTDPSVIYGLGERFDGNLKRVHLRESTPYNTYVIRGLPP
                     TPIALPGGKSIDAALHPGPGDYLYFVSRGDGTSQFSRSHAEHLAAVRKYQLK"
     gene            4392..5033
                     /gene="tmk"
                     /locus_tag="AC16QF_14415"
     CDS             4392..5033
                     /gene="tmk"
                     /locus_tag="AC16QF_14415"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR6449802.1"
                     /translation="MSVDSGSRGRFITLEGTEGVGKTSNLEHVCHLLQRAGYEVVATR
                     EPGGTPLAESIRSLLLEVREEPVDPTVELLMVFAARAQHVARVIEPALAAGRWVVCDR
                     FTDATFAYQGGGRGISMEDIATLESLVQGDLRPDLTLYLDVPVEVAAKRIAGRDHDRF
                     EREQMDFFERVRKTYLERAADEPQRVRVVDARQDLATVQAEIDRVVAEFLDRS"
     gene            5030..5935
                     /locus_tag="AC16QF_14420"
     CDS             5030..5935
                     /locus_tag="AC16QF_14420"
                     /inference="COORDINATES: protein motif:HMM:NF024575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449803.1"
                     /translation="MTALLPWHVTLADRIEGLMSAGRLPHGILITGTEGWGDSILAAW
                     LALRILAEDTDQNPRELAHPNLRWIVPDGAEIKVSAVRELGEFAFSTSQGGGAKVAVL
                     ENAHLLNRNAANALLKTLEEPPANTFLVLATSHPGRLLATIRSRCQRFVIQPDAQAAR
                     GWLQGRWSEAELASRLPETGGAPLLVDAALTEGSTALRECLEVALSSPRPLDSIAELL
                     ERDAGQVTSGWYRTLRDLIAGREEIGALRQTDSRALFEFADELLWVRHQLVNTNSANA
                     RLLFERLVAKWCSLGMESARRPGNT"
     gene            6020..6370
                     /locus_tag="AC16QF_14425"
     CDS             6020..6370
                     /locus_tag="AC16QF_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR6449804.1"
                     /translation="MAARRGARNGILSLAIKDKAVLYAAYMPFVKNGGLFIPTKKDYE
                     LGDEVFMLLNLMDEAEKIPVAGKVVWVTPSGAQGNRAAGIGVQFNDQDDVARSKIETY
                     LAGALQSDRHTHTM"
     gene            6377..7138
                     /locus_tag="AC16QF_14430"
     CDS             6377..7138
                     /locus_tag="AC16QF_14430"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009406818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR6449805.1"
                     /translation="MLVDSHCHLNYLDDPDSRLQAARLAGVDGFLCIGVDKDGIAAVL
                     DIAERHLDVWASVGQHPEAAESPPDWVSDYLTHPRVVAVGETGLDYHHGRQAHIQRLQ
                     RERFAWHLEIAGVAGLPVVVHTRDAVADTADLLRAHRDVGGVLHCFTETWDLAKSALD
                     LGYYISISGIVTFRNGENVREVASRVPRDRLLVETDCPWLAPAPHRGRTNEPAFVRDT
                     ATFLADHLNWDRDALAAQTSENFFRLFACGPQTAA"
     gene            complement(7135..7602)
                     /locus_tag="AC16QF_14435"
     CDS             complement(7135..7602)
                     /locus_tag="AC16QF_14435"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6449806.1"
                     /translation="MSERTTNVSVRSAGSGDASLLIEFNLAMAMESEDKGLDRATLAA
                     GVEHLIEHPLDGFYLVAELAGVPAGSLMVTYEWSDWRAGRFWWIQSVYVKPEYRRKGV
                     YSALHQSVRERALSAPDTCGIRLYVERENAGAQATYASLGMSETAYRLYEEEF"
     gene            complement(7599..9725)
                     /locus_tag="AC16QF_14440"
     CDS             complement(7599..9725)
                     /locus_tag="AC16QF_14440"
                     /inference="COORDINATES: protein motif:HMM:NF013123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6449807.1"
                     /translation="MKLTVERLFSDPPLTGSVPAAGQFSPDGRRAAFLRTAEDDRERQ
                     DLWRLDCGNGDLACWLDARQIDDQGSSPTAAEKAERERRRQFQGGISGFAFSPGGDKV
                     LVTAAGAGYLMDTANGRVDRFTPAGTRQTDLRFSPDGRHITYVRDGNLLCWDLAASSE
                     RAITNDGGGTVSYGAAEFIAQEEMHRFDGYWWSPDGTRIAYTRVDESPIPVTNRLEID
                     ADQIRTVEQRYPFAGGANAAVALLVMDWASGAVTEISYLQTPSDYLARVNWAGSELAV
                     QTQSRDQRELRLRFFDPVSGDSRDGLEERSATWINLHDNFRYLEGERFLWTSERDGHS
                     HLYLCDGSRELPLTRGEGRVNRVLHADEHRILFTGWFGNPQEQHLYSVSASAASGPEE
                     PVRLTAEPGWHEVTVDLRGEQFLDRFSAVDRQPSCRVRKLEGPQVNTIGQELADGLSN
                     YAPYLTHHVTPQLGTVVCEDGQTLHYRLTRPSNGSRQKSPVIVHVYGGPGVQRVRNEW
                     APALLQLFVQHGFGVFELDNRGSGNRDRGFEEPIHRQLGKVEVADQVLGADFLRSLDW
                     VDPERIGVFGHSYGGYMTIMCLAQAPASFKAGVSVAPVTDWHLYDTHYTERYLGHPED
                     NASGYAASSVFPHLDGIKGRLLLIHGMADDNVLYTNSTMLYRALQARHFPFEMMAYPG
                     SKHSLQERDVSIHRFNLILDFFERAL"
BASE COUNT         1761 a         3039 c         3246 g         1815 t
ORIGIN      
        1 caggaaagtc ctcgagcagg aagactgacg caccgtgagt ctcggtttca tgttccccgg
       61 gcagggctcc cagaccgttg ggatgctctt ggacatctcc gccgagtatc cgcaggtgat
      121 tgagtgtttt cagcgcgcgg gcgacagcat cggccggccg ctgtgggaga taacgacgtc
      181 cgggccggat acggagctca acagcacgga gatcacccag ccggcgctcc tgacggcgag
      241 cgtcgcgctc tgggagatct ggcagacgtc gggaggagaa gcgcccgcga tgctttcggg
      301 ccacagcctg ggcgagtatt cggccctggt ttgcgctggt tcgctttccc tcgaagacgg
      361 ggtgcggctc gttcacgagc gtggcaagct gatgcagaag gccgtgcctc acggtaaagg
      421 cgccatggcg gccgtgctcg gtctcgacga cgatgtcgtg gctgcgtgct gcgaggccgt
      481 ggacggggtc gtctccccag ccaacttcaa cgcgccgggc caggtagtca tcgctggagc
      541 cacggccgcg gtggagtcgg ccgtagaagc ctgccgcgag gcgggtgccc gacgcgccgt
      601 gctgctgccg gtgagcgggc cctttcattg cgagctcatg aggccggttc agaacgcctt
      661 tgccgacgtg ctgggccgcg tcgacctcgt catgccgagt attcccatcg ttcacaacgt
      721 cgatggagag gtcgcgcccg acctggatgc gttgcgatca aaactgatgg cccagatcgc
      781 cgagcccgtg ctctggacgc gctgcgcgca gaacatggtc gctgccgggg tcacccggct
      841 tgtggaatgc ggtcccggca aggtgctcag tggcctcgtc aagcgaatcg acaagtcggt
      901 ccaggccgaa tctctgggca ccctcgacgg gttgcgcgcg gcgctcggtt agttggcggc
      961 ggaacgtaaa gtagcgctgg tgaccggcgc atcgagaggg atcggggctg ccatcgccca
     1021 acgtctgtct ttagaccatc acgttgtcgg aaccgccacc tcggagcagg gtgccctggc
     1081 gatctccgag tccctcggcg atgccggcga gggtctcatc atgcgcgtgc aagacgatgc
     1141 ctcggtgtcg gccgccctgg accggctggc ccaggggtgc gggatgcccg ccgtcgtcgt
     1201 gaacaatgcc ggcataacgc gcgacaacct tctgctgcgg atgaaaccgg aagaatggca
     1261 ggaagtaatg gacacgaacc tgggcgcgct ttaccgcgtg gtgaagccgt gcttgcgcag
     1321 catgatgaaa tcgcgatggg ggcggatcgt gaacctgagc tccgtggttg cccggatggg
     1381 aaaccctggc cagagcaact acgtagcgag caaagccggc atcgaaggct tcacgcgatc
     1441 gctggccatc gaggttgctt cccggggaat caccgttaat gcggtcgcgc cagggttcat
     1501 cgagaccgac atgacctcgc agctgtcaca agaccaggtc gaggaaatgc tggcgcgggt
     1561 gcctgctggc cggatgggat ctgcggacga agtcgccgaa gccgttgctt atcttgttag
     1621 cgacgcggcg ggctatatta cgggaaccac gcttgccatc aacggcgggc tgcacatgac
     1681 ttgaaaagcg acgaaatttg agctttcgga ttccggattc aagcgattcc gggtgcccga
     1741 gcctcaactt gaattggcca gggggggtct atagaattcg gcgctccctg cacccaggaa
     1801 cctgattggg agatgactgc agcatgagca gtgtagaaga acgcgttaag aagctgattt
     1861 gtgagcagct gggagtcaaa gaggacgagg tcaaggacga agcttcgttc gtggaggatc
     1921 tcggtgcgga ttctctggat acggtcgagc tggtgatggc gctggaagaa gagttcgaga
     1981 ccgagattcc cgacgaagaa gccgagaaga tcaccaccgt caaagaggcc atcgactata
     2041 tcctggcgca tcagtagccg gcgaaccctt gtaattcacg tctaagccgc atctacttgg
     2101 gatggtgcgg ctttttgttt ctgggtaatc gagagatcga aacatgacca gacgacgcgt
     2161 ggtggttacc ggtattgggc ttctcacgcc gatcggcaac gacgtcgact ctgcgtggtc
     2221 gaacgcgctg gcgggcaaga gcggcgcagg gcccatcgaa cagtttgatg ccaccgactt
     2281 cagcgtgagg atctgcgcac ccgtcaaagg gttcgacgcg acgcagtacg tagaccgcaa
     2341 ggaagtgcgc cgcaacgatc cgttcatcgt ctacggcatg gcagcgggcg tgcaggccat
     2401 cgaggatgcg gggctcgacg cgcatccggc cgacggccac cgttacggtt tagccatcgg
     2461 atccggtatc ggaggtctgg ggaccatcga ggacacacac ggcagcctcg tcgctggcgg
     2521 tccgagaaag gtttccccgt ttttcattcc tgccagcgtg atcaacatga tcagcggcaa
     2581 cctgtccata cgctacggat tcaccgggcc caacgtggcg gtcgtgactg cctgcaccac
     2641 cggaacccac aacatcgggt tcggggcgcg gatgattcag agtggcgatg ccgacgtcat
     2701 ggtggtcggg ggggcggagt acgcgacgac ccccgtggcg gttgcggcat tctcgatgat
     2761 gaaggcgctc tccacgcgga acgacgagcc ggagcttgcc agccggccct gggatcgtga
     2821 tcgggacgga ttcctgctcg gggaaggggc gggcgtcctt gtcctcgagg agtacgaacg
     2881 ggcaaaggcc cgggacgcca agatctatgc cgaggtggtt gggttcggga tgagcgccga
     2941 tgcctttcac atcaccagcc ccgcggagga tggcgcaggc gccgctgcgt gcatgcagaa
     3001 cgcgcttgca gacgcgggcc tgcgttcgga tcagatcgac tacataaacg cccacggaac
     3061 atccacgccg ctcggcgacg tggcggagac ggtggccatc aaacgcgtct tcggagacga
     3121 cacccgggtg gccgtcaatt ccaccaagtc catgatcggg catctgcttg gcgcggcggg
     3181 cagcgtggaa gcgatcttta cgattctcgc catccgcgat caggtgtctc cgccgaccat
     3241 caacctggac aatcctggcg aaggctgcga tctggactac gtcccgaacg tggccagaca
     3301 gatgcagatc agggctggtc tgagcaattc gttcggattc ggcggaacca acgggacctt
     3361 gatcttcgca tcggtctgaa cgacgcgcct gatgtagaat ccggcgcgat gaggctcttc
     3421 ctggtcagct tcgccgcctt gatccttctc tccgggtccg cagcattcta tgtggttcgg
     3481 tggaatgatg ccccgctcgc cctgaccgag ccggtggtgg tcgtgctcga ggcgggcgaa
     3541 ccgttttcca agttcgcagc ccggataagc gcgtcggggt acctcacaca cccaaggctc
     3601 tggtcccggt tggctcgtct gcgcggtctt gccaggcagg ccaaggccgg agagtacgcg
     3661 ttgcagcctg gggatacgcc cgaacggctg ctgcagcgga tcgtccgcgg tgaggtcgtt
     3721 acttatgaag tccggctgct cgagggctgg accgtgatgc aggtgctgct cgcgttgcag
     3781 gagaaccagg tgctcgagca cgaactcgtc ggcgcgaacg tcaccacgct gctggaagcc
     3841 ctgggactgc caggaggtca tgcagagggg ctcttctatc cggacaccta tcagttcgtg
     3901 cgcggttcgt ctgatcggga catcctgcgc agagcctacg accggatgca gatggttctc
     3961 gagggcgcct gggaccagcg cgcgtcgggc cttccctacc gaactccata cgaagcgctc
     4021 atcgtggcat ccctcgtgga gaaggagacg ggtcgcgacg aggatcgcga gcacatcagc
     4081 caggtgttcg tcagccgact gaatgccaac atgcgcctgc agacggaccc gtctgtgatt
     4141 tacggtctgg gcgagcggtt cgacggaaac ctgaaacggg ttcatctgcg cgagtcgacc
     4201 ccttacaaca catacgtgat cagggggctc ccgccaacgc ccattgctct tcccggtggc
     4261 aaatcgatcg acgcggcgtt gcatccgggc cccggggatt acctgtattt cgtctcccgg
     4321 ggggacggta ccagccagtt ttcccgaagc cacgccgagc atctcgccgc ggtgcgtaag
     4381 tatcagctga aatgagcgtc gacagcggca gcaggggtcg gttcatcacc ctcgaaggga
     4441 ctgagggcgt cggcaagacg agcaatctgg agcacgtctg ccatcttctg cagcgtgccg
     4501 gatacgaagt cgtcgcgacc cgtgagcctg gcggcacgcc gctggccgag agcattcgat
     4561 cgttgctgct cgaggtgcgc gaggagccgg tagatcccac cgtggagctt ctgatggttt
     4621 tcgccgcccg cgcccagcac gtcgcccgcg tcatcgaacc ggcgctggcg gccgggcgct
     4681 gggtggtgtg cgaccggttt acggacgcca ccttcgctta tcagggcggc gggagaggca
     4741 tctcgatgga agacatcgcc accctggaat ccctggttca gggggatctg cgtcctgatc
     4801 tgacgctcta tctcgacgtg cccgtcgagg tggctgccaa gcgtattgcc gggcgggatc
     4861 atgaccgctt cgagcgcgag cagatggact tcttcgagcg cgtgaggaaa acctacctcg
     4921 agcgggcagc ggacgaaccg cagcgcgtgc gggtcgtgga tgcccggcag gacctcgcga
     4981 cggtgcaggc ggaaatcgac cgcgtcgtcg ccgaattcct ggatcgctca tgacggcact
     5041 actgccctgg catgtgacgt tggccgatcg tatcgagggc ctcatgtctg ccggccggct
     5101 tccgcacggc atcctcatca ccggcaccga aggatggggc gacagtattc tggcggcctg
     5161 gctggcactg cggattcttg cagaggatac cgatcagaac ccccgggagc tggcgcatcc
     5221 gaatctgcgc tggatcgttc ccgacggtgc ggagatcaag gtcagcgcag tgagagaact
     5281 cggcgagttc gctttttcca ccagccaggg cggcggtgcc aaggtcgccg ttctggaaaa
     5341 cgctcacctg ttgaatcgca acgcggcgaa cgcgcttctg aagaccctgg aggaaccgcc
     5401 ggccaacacg tttctcgtcc tggcgacgag ccatcctgga cggttgctcg cgacgatccg
     5461 cagccgttgt cagcggttcg tcatccagcc ggatgcgcag gctgcccgtg gctggctgca
     5521 gggtcggtgg agcgaggccg agctggcctc gcggctgccg gaaaccggcg gcgcgccgct
     5581 gctcgtggac gcggcgctca cagagggctc gacagccctg cgcgaatgcc tggaggtggc
     5641 gctgtcctca ccacggcccc tggacagcat cgccgagctg ctcgagcggg atgcaggcca
     5701 ggtcaccagc ggctggtacc gcacgctacg cgacctgatc gccggccggg aggagatcgg
     5761 cgctttgcgg cagacggaca gcagggcgct gttcgaattc gccgacgagc ttctatgggt
     5821 gcgccaccag ctcgtcaaca ccaacagcgc caacgcccgg ctgctctttg agcggctggt
     5881 ggcgaaatgg tgcagccttg ggatggagtc ggcgcggcga ccgggcaata cgtgatgccg
     5941 ttctcggtct tgcgattgac agttttcgcc cgcgcgcgga tcatcatctg cgttgtacgg
     6001 ggcacaaggt attgtccaga tggcggctcg tcgcggcgct agaaacggaa ttctctccct
     6061 cgcgatcaag gacaaggcgg tgctttatgc cgcttacatg cctttcgtga aaaacggggg
     6121 gctgttcatt cccaccaaga aggactacga gctcggcgat gaggttttca tgcttctcaa
     6181 tctcatggac gaagcagaaa agattcccgt ggccggcaag gtcgtctggg tgacgccctc
     6241 gggcgcgcag ggaaatcgcg cggccggaat aggcgtgcag ttcaacgatc aggacgatgt
     6301 tgcccgctcc aagatcgaaa cctacctcgc cggtgcgctg cagtccgaca gacacactca
     6361 caccatgtga acggccttgc tggtcgattc tcactgtcac ctgaactatc tcgatgaccc
     6421 ggactcccgt ctgcaggcgg cgcggcttgc cggcgtcgat gggtttctgt gcatcggggt
     6481 ggataaagac ggcatcgccg ccgtgctcga cattgccgag cgccaccttg atgtctgggc
     6541 gagcgtcgga cagcatcctg aagccgccga gagcccgccg gactgggtca gcgactacct
     6601 gactcatccc cgggtcgtcg ccgtggggga aacgggcctg gactaccacc atggtcgcca
     6661 ggcgcatatc cagcgcctgc agcgggaacg gtttgcctgg catctcgaga ttgcgggcgt
     6721 cgctggactg cccgtcgtcg tgcatacccg cgatgcggtg gcggacacgg ccgacctcct
     6781 gcgggcgcat cgggacgtgg gaggcgttct gcactgcttt acggagacct gggacctcgc
     6841 caagagcgcg ctcgacctcg ggtactacat ctcgatatcc ggcatcgtga cgtttcgaaa
     6901 tggtgagaac gtccgcgagg tggcgagcag ggtgccccgt gaccgtctgc tcgtagaaac
     6961 cgactgcccg tggttggcgc cggcaccgca ccgtggccga accaacgagc ccgccttcgt
     7021 tcgtgacacg gcgacgtttc tcgcggacca cctgaactgg gatcgggacg cgctggcagc
     7081 gcagaccagc gaaaacttct ttcgcctctt cgcctgtggg ccgcagacgg cggcctagaa
     7141 ttcttcctcg taaagccggt aggcggtctc tgacatgccg aggctggcat aggttgcctg
     7201 ggcgcctgcg ttttcccgct cgacgtagag ccggatgccg cacgtgtcgg gtgctgaaag
     7261 cgcccgttcg cgcacgctct gatgcagcgc cgagtagacg cccttgcggc gatactccgg
     7321 tttcacgtac acgctctgga tccaccagaa tcgtcccgct ctccagtcgc tccactcgta
     7381 ggtgaccatc agggagccgg ccggcacccc ggcaagctct gctaccagat aaaacccgtc
     7441 gagcgggtgc tcgatgaggt gctccacgcc cgcggccagc gtcgcgcgat ccaggccctt
     7501 gtcctcgctc tccatcgcca tggccaggtt gaactcgatc agcaggcttg cgtcgccgct
     7561 gcccgccgac cttacgctga cgttggttgt tctttcgctc acagcgcccg ctcgaagaaa
     7621 tcgagaatca gattgaaacg atgaatggag acatcgcgct cctgaagtga atgcttggac
     7681 cccgggtagg ccatcatctc aaaggggaag tgccgggcct gaagcgcccg gtagagcatg
     7741 gtgctgttcg tgtagagaac gttgtcatcg gccatcccgt gaatgagcag cagcctgcct
     7801 ttgatcccgt cgagatgagg aaatacgctg ctggcagcgt acccggaagc attgtcttcg
     7861 gggtgcccga gataacgttc cgtgtagtga gtgtcgtaaa gatgccagtc ggtcaccggt
     7921 gcgaccgaaa cgccggcttt gaacgacgca ggggcctggg ccaggcacat gatggtcatg
     7981 tagcccccgt agctgtgccc gaaaacccca atgcgttccg gatcaaccca gtcgagcgag
     8041 cgaaggaaat cggcgccgag cacctggtct gccacctcga ccttgcccag ctggcggtga
     8101 atgggctctt cgaacccgcg gtcgcggttc ccggaacccc ggttgtccag ctcgaacacg
     8161 ccgaacccgt gctggacgaa aagctgcagc agcgcaggcg cccattcgtt acgcacccgc
     8221 tgcacgcctg gcccgccgta cacatgcacg atcaccggac tcttctgtcg gctcccgttt
     8281 gatggtcgcg tgagccggta atgcagggtc tggccgtcct cgcagacgac ggtgccgagc
     8341 tgcggtgtca cgtggtgggt gaggtagggc gcgtagttcg aaagcccgtc cgcgagctcc
     8401 tgcccgatgg tgttcacctg gggcccctca agcttcctga cccgacagga cggctgccgg
     8461 tcgacggcgc tgaagcggtc gagaaactgc tcgcccctga ggtcgacggt aacctcatgc
     8521 cagcctggct ccgccgtcaa cctgacaggc tcctccggcc ccgaagcagc ggacgcgctg
     8581 accgagtaga gatgctgctc ctgggggttg ccgaaccagc cggtgaacag gatgcggtgc
     8641 tcatccgcgt gaagcacgcg gtttaccctt ccctcgccgc gagtcagcgg cagctcccgg
     8701 ctcccgtcac agaggtagag atggctgtgg ccgtcgcgct ccgacgtcca gagaaagcgc
     8761 tcgccttcga ggtagcggaa gttgtcgtgc aggtttatcc aggtggccga tctttcttcc
     8821 agcccgtctc tggagtcccc cgataccggg tcgaagaagc gcaaccgcag ttcccgttga
     8881 tcccggctct gtgtctgcac cgccagctcg ctcccggccc agttcactct cgccaggtag
     8941 tcgctcggcg tctgcaggta cgaaatttcg gtaaccgcgc cggacgccca gtccattacc
     9001 agcagcgcga cggccgcgtt ggcgccgcct gcaaacggat agcgctgctc cacggtgcgg
     9061 atctgatcgg catcgatctc caaccggttg gtgacgggga tgggcgactc gtccactcgg
     9121 gtgtaggcga tcctggttcc gtcgggcgac caccagtagc cgtcgaatcg gtgcatctcc
     9181 tcctgggcga tgaactctgc cgctccgtag gagacggtgc ccccgccatc gttggtgatg
     9241 gcgcgctctg acgaagctgc gagatcccag cagagcagat tgccgtctct gacataggtg
     9301 atgtggcgcc cgtccggcga aaaacgcagg tccgtctggc gagttccggc aggcgtgaac
     9361 cgatcgacgc ggccgttggc cgtatccatc agatagccgg cgccggcagc cgtgaccagc
     9421 accttgtcac cgccggggct gaacgcaaag ccggagatcc cgccctgaaa ctggcggcgg
     9481 cgttcgcgtt cggctttttc ggccgccgtc gggctcgatc cctggtcgtc aatctgtctt
     9541 gcatccagcc agcaggccag gtcaccgttg ccgcaatcca ggcgccacag gtcctggcgc
     9601 tcgcgatcgt cttcggcggt gcgcagaaat gccgcccggc ggccgtcagg agagaactga
     9661 cccgcggcgg gcacgctgcc cgtcaacggc ggatcgctga aaagccgctc gacggtcagc
     9721 ttcatagatt ggcgaaggcg tttcgcgtca ggttctcgac tccgttcgtg cccaccaaca
     9781 cgttgtcttc gatgcgaata ccgccgtggg gcagaaaggc gtccacgcgg tcccagttga
     9841 cgacgtgggc gtgctcgctt g
//
LOCUS       DBNHLM010000315         3398 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1184515, whole genome shotgun sequence.
ACCESSION   DBNHLM010000315 DBNHLM010000000
VERSION     DBNHLM010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1184515"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..885
                     /locus_tag="AC16QF_14445"
     CDS             139..885
                     /locus_tag="AC16QF_14445"
                     /inference="COORDINATES: protein motif:HMM:NF018038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR6449808.1"
                     /translation="MSGPINAYLVANARYHDTDFAALELLKLLAEDDNIQTKVADSFA
                     NVEAIAASDFLITYTCDLRPTHEEEVGLREFVSNGGRWIALHGTNALLDFTDEGQVIA
                     PRINDTFMETLGSRFISHPAIEPYTVTVSNPDHPMVRGIEPFEATDEIYLCEYYGKVE
                     PLLETRYTGTFEAGYVENEWPDDDPRLVAYTHRVGEGEVLYITLGHCCGKYDMQPIVE
                     EAPVVRGSWDNPIFYELLRRAIRWAKDELN"
     gene            complement(927..1316)
                     /locus_tag="AC16QF_14450"
     CDS             complement(927..1316)
                     /locus_tag="AC16QF_14450"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR6449809.1"
                     /translation="MIYLDTSVALAHLLAEDRRPPDWIWQETLVASRLMEYELWNRLH
                     VMGFAASHGEAARGLVSQIALLELSPEVLQRALEPYPVRIRTLDALHLASLGFLQTHG
                     QKVELATYDTKMADAARAMRMSLIDLP"
     gene            complement(1313..1606)
                     /locus_tag="AC16QF_14455"
     CDS             complement(1313..1606)
                     /locus_tag="AC16QF_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR6449810.1"
                     /translation="MKAVGIKVLKNKLSEYVRLAAGGETVLVTDRDRVVAQITAPGPT
                     VSPLLADAALADAVRNGHIRAPLLRTGQPPVAPPPALKLSDVLEGLEQDRGER"
     gene            complement(1803..3194)
                     /locus_tag="AC16QF_14460"
     CDS             complement(1803..3194)
                     /locus_tag="AC16QF_14460"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6449811.1"
                     /translation="MVKHASTDAAGRDAVPPLSRRTLFYYGLADMPIQMAAVPVAAFV
                     PNYYGQDLGVSLAAVGLVLLLSRSFDAITDPLIGWLSDRTDTRWGRRRVWMLASVPIL
                     MLAIYKLFMPDPGDASAVYLLIWLVVLWLGWTMLFIPYYAWAAELSPEYNERSRITGW
                     RSSIGLAANVLSKLVPVVAIFCCAYGGTQETLFLVGTMTLILLPLTVGLTVAMVPEGK
                     DFIPARMPLVPGLKIMWRNGPFKRLVAAFFVNQLGTSISTALIVFYVRSVLQDDDKTI
                     LQLLVYYGFNLCGIPFFVRYSGIVGKHRAWCIALLLFAFFMLGYLLLGAGDFYWMLPI
                     TAITGFLAGSFWVIPNSMKADVIDIDRLESGEDRTAWYFAVWSLATKVALSVGPAIAL
                     GLLSLTGYVPAAEAGAYEGSTLGLKLLFVFGPSTGLLLTALIAWRYPLTETKHRELRQ
                     ALARSRSRPRPAG"
     gene            complement(3178..>3398)
                     /locus_tag="AC16QF_14465"
     CDS             complement(3178..>3398)
                     /locus_tag="AC16QF_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449812.1"
                     /translation="WLALTLLAFVGYDPASQPEPTAGGLLGLKLLYVFGPACGFWLAA
                     LIAWNHPLTESRHREIRASLEKVRGQAR"
BASE COUNT          654 a         1089 c         1120 g          535 t
ORIGIN      
        1 cctctcccgg tgggagaggg ccctactatc tcaatctgaa gcgagcgtgt tcgaaagccc
       61 accggcgcgc atctgcggat gtgttaccgt tggccgccgc catgctttcg tttgccgggt
      121 acatttcgga ggatactcat gtccggaccc atcaacgcct atcttgtcgc caacgcgcgc
      181 taccacgaca cggacttcgc tgctctggag cttttaaagc tcctggccga ggacgacaac
      241 atccagacca aggtggccga cagctttgcc aacgtcgagg ccatcgccgc gtcggatttc
      301 ctgatcacct acacctgtga cctgcgcccg actcatgagg aagaagtggg gctgcgggaa
      361 ttcgtgagca acggcggccg ctggatcgcg ctgcacggca cgaatgccct gctcgacttc
      421 acggacgaag gccaggtgat cgccccgcgc atcaacgaca ccttcatgga gaccctgggc
      481 agccgcttca tctcgcaccc cgccatcgag ccctacaccg tgacggtctc caaccccgac
      541 caccccatgg tccggggcat cgagccgttc gaggctaccg acgagatcta tctgtgcgag
      601 tactacggca aggtggagcc gctgctggag acgcgctaca cgggcacgtt cgaggccggc
      661 tacgtggaga acgagtggcc cgacgacgat ccccggctgg tggcgtacac ccatcgggtg
      721 ggcgaagggg aggtgctcta catcaccctc ggccactgct gcggcaagta cgacatgcag
      781 ccgatcgtgg aggaagcccc cgtggtcagg ggatcctggg acaaccccat cttctacgag
      841 ctgctgcgcc gcgccatccg ctgggcaaag gacgagctga actagcggcg tagccgccga
      901 agcctctctt tcgctgctga gggcgtttac ggcaggtcga tgaggctcat ccgcatggcc
      961 cgcgcggcgt cggccatttt cgtgtcgtaa gtcgccagtt ccactttctg gccgtgggtc
     1021 tgcaggaagc ccagcgaggc gaggtgcaaa gcatccagtg tccggatcct gaccgggtac
     1081 ggctccaacg cccgttgcag cacctcgggc gagagttcga gcaacgcgat ttgcgacacc
     1141 agccccctgg cggcttcgcc gtgggatgcc gcaaacccca tgacgtgcag ccggttccac
     1201 agctcgtact ccatgagtcg gctggccacc agggtctcct gccagatcca gtcagggggg
     1261 cggcgatcct cggcgagcag gtgggccagg gcgacggacg tatccaggta aatcaccgct
     1321 cgccgcgatc ttgctcgagg ccttccaaaa cgtcgctcag ctttagcgcc gggggagggg
     1381 ccactggcgg ctggccggtg cgcagaagcg gggcgcggat gtggccgttt cgaactgcgt
     1441 ccgccagggc ggcgtctgcg agcaatgggc tgacagtggg ccccggcgcc gtgatctgcg
     1501 ccacgacgcg atcgcgatcg gtcaccagaa cggtttcgcc gcccgctgcc aggcgcacgt
     1561 actcgctcag cttgttcttg agtactttga tgcctacggc cttcatttca caaaagtagc
     1621 tactagtagc taccaatgca accgggattt tcgtctgccc gaaaaaacta ctcgagcgcg
     1681 gctccctgcg gaaccggcag cgcttgtacg agtatgtagt ctgtcgccgg aggcgagcgg
     1741 cagccgaagc cgcctctagc ctctccaccg ctggacgggg cctttacagg agcgcgctga
     1801 gttcaacccg ccggccgcgg cctgctgcgc gagcgggcga gagcctgtct cagttcccgg
     1861 tgcttggtct cggtgagcgg gtagcgccac gcgatcagcg cggtgaggag gagcccggtg
     1921 gacggcccga agacgaacag cagcttgagc cccagcgtcg agccttcgta agcgcctgcc
     1981 tcggcagccg gcacgtagcc ggtgagggag agcagcccca gagcaatagc cggcccgact
     2041 gacagggcga ccttggtggc cagggaccag acggcgaagt accaggcggt gcggtcctcg
     2101 ccgctctcca gccggtcgat gtcgatcacg tcggccttca tggagttggg gatcacccag
     2161 aaagacccgg cgagaaaacc cgtgatcgcc gtgatgggca gcatccagta gaagtcgccg
     2221 gctcccagca gcaggtagcc cagcatgaag aaggcgaaca gcaacagagc gatgcaccag
     2281 gctcgatgct tgccgacgat gccggagtag cgcacgaaaa acgggatgcc gcagaggttg
     2341 aacccgtagt acaccagcag ctgcaggatg gtcttgtcgt cgtcctgcag cacgctgcgc
     2401 acgtagaaga cgatgagcgc cgtggagatg gaggtcccca gctggttgac gaagaacgcg
     2461 gcgaccagac gtttgaaggg cccattgcgc cacatgatct tcagccccgg caccaggggc
     2521 atgcgggccg ggatgaagtc cttgccttcg ggaaccatgg ccaccgtcag ccccacggtc
     2581 agtggcaaaa gaatcagcgt catggtgccg acgaggaaca gggtctcctg ggtgcccccg
     2641 taggcacagc agaagatggc caccacgggt accagcttgc tgagcacgtt ggcggcgagc
     2701 ccgatgctgg accgccagcc cgtgatgcgg gaacgctcgt tgtactccgg agacagctcg
     2761 gccgcccagg cgtagtaggg aatgaacagc attgtccagc cgagccacag aaccaccagc
     2821 cagatcagca ggtagacggc ggaggcgtcg ccgggatcag gcatgaagag cttgtagatg
     2881 gcgagcatca ggataggcac ggaagccagc atccagaccc ggcgacggcc ccagcgcgta
     2941 tccgtgcggt ccgacagcca gccgatcagc ggatcggtga tggcgtcgaa cgatcgcgag
     3001 agcaggagca ccagcccgac ggccgccagg ctcacgccca gatcctggcc gtagtagttg
     3061 ggcacgaagg cggccaccgg caccgcggcc atctggatgg gcatgtcggc caggccgtag
     3121 tagaacagcg tacgccgcga aagcggcggc acggcgtccc ggccggctgc gtcggtgcta
     3181 gcgtgcttga ccacggacct tctccaggct ggcgcgtatc tcccggtggc gcgactcggt
     3241 gagcggatga ttccaggcaa tgagggcagc cagccagaag ccgcaggccg gcccgaacac
     3301 gtaaagcagc ttgagtccca gcaggccacc cgccgtgggc tccggctggc tggccggatc
     3361 gtagcccacg aaggccagaa gcgtcagcgc gagccagg
//
LOCUS       DBNHLM010000316         5616 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_526533, whole genome shotgun sequence.
ACCESSION   DBNHLM010000316 DBNHLM010000000
VERSION     DBNHLM010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_526533"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="AC16QF_14470"
     CDS             <1..955
                     /locus_tag="AC16QF_14470"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HIR6449813.1"
                     /translation="DAKYSSAVITKALTDEGFSNVRCVNDGFAALDTLENRSAQILIA
                     DWVMPAMDGLELTRRVKRLDEENGHFTYVMLLTGREDFEGMLEAFDTGVDDFLNKHNL
                     RAELQPRIIAAQRIAARQNELLRTNKLLRKKVRELSVVDLIDPVTGLGNRRFTIERLT
                     AAMEQAESRRMAACLLLVGVDNVAALTERYGPPAVDELMSGIGSKIRTLVRPLDLVTR
                     PEPGIFAVITLHPSIDECTSSTFQRIFDGLYLNSFKTSEAYIPVSVGISITAADAQTG
                     FPHPRQLMRQAVHALGESFESGQIEVQRAATLNEPVTAAEL"
     gene            1000..1533
                     /locus_tag="AC16QF_14475"
     CDS             1000..1533
                     /locus_tag="AC16QF_14475"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR6449814.1"
                     /translation="MTTKAGDTGKTSLADGSKLAKTEPRMHAIGTVDELNSFIGLLVT
                     QLDPDAALREPCARIQQELFDIGATLATVGAIPCPDNAWIDEEVQTLNARLPPLTEFV
                     IPGGTVAAALAHVCRTTCRRAERELWALQDDDSATAAARYTNRLSDLLFVMARTLNAA
                     EGAEPQWRGTRGPKGPE"
     gene            complement(1552..3234)
                     /locus_tag="AC16QF_14480"
     CDS             complement(1552..3234)
                     /locus_tag="AC16QF_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HIR6449815.1"
                     /translation="MATEIRIPDIGDVEHVEVVEICVAAGAEVAADDALIIIESDKAS
                     MEVPAGVAGVVASLAVAVGDTVAEGDLIAVIEAAGAAPVEDQAGEVAAEAPPQMQDPK
                     VPPERAVASPPTKPEAGPSGTVEIRVPDIGEAEDVVVVEVAAAAGQQVGEDDLLVVVE
                     SDKASMEIPAGCSGTVLEMHVSEGAAVTEGTLIATVEVKGSSAAPAPAADRPEVEAGE
                     VPRGAEAAAPQPISTTAAPPAGPDTAAAEQESAGTALGERIYAGPAVRRLARELGVDL
                     ARVRGTGARERIVKDDVKAYVKDAMTAAPGGAGAGAASGAGIPAIPQIDFSRFGPVEI
                     RPLTRIRAAGAANLQRSWLNLPHVTQHDEADVTELEAFRASLKSEAERRGVKITPLAF
                     IIKACCYALKKFPTFNASLDAEARNYVLKGYYNIGFAVDTEEGLVVPVIKGADQMGIW
                     ELSEAVTDLAGRAREQKLGMDDMQGGSFSVSSLGAIGGTGFTPIINAPEVGILGVSRL
                     AMRPVWDGESFVPRKLLPLSLSYDHRAINGAEAGRFVSHLCELLADIRRLSL"
     gene            complement(3247..>5616)
                     /gene="aceE"
                     /locus_tag="AC16QF_14485"
     CDS             complement(3247..>5616)
                     /gene="aceE"
                     /locus_tag="AC16QF_14485"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HIR6449816.1"
                     /translation="RRPGDLGGHISSFASSATLYDVGFNYFFRGPTDEHPGDLIYFQG
                     HASPGIYARSFLEGRLSEAQLDNFRREVAGEGLSSYPHPWLMPDYWQFPTVSMGLGPI
                     QAIYQAHIMKYLDARGLLPMGERKVWAFLGDGECDEPEALGALSKAGTEKLDNLIFVV
                     NCNLQRLDGPVRGNSKIIQELEGNFRGAGWNVIKVVWGRLWDPLFANDTAGLMQLRMN
                     EVVDGEYQNYKAKGGAYTREHFFGAHPELLAMVERLSDDDIMRLNRGGHDPFKVYAAY
                     HEAVHHKDEPTVILAKTVKGYGMGDAGEAENDTHQVKKLNLDELKRFRDRFDVPLSDT
                     ELEDIPFYRPPKDSPEMVYLRKKRADLGGPIPQRTTASEALQVPELSAFAQQLQGTGD
                     RSNSTTMAFVRMLGTLVRDKNIGKRIVPIVPDEARTFGMEGMFRQLGIYSSAGQLYEP
                     EDYGEIAAYRESRDGQVLEEGINEAGAFSAWLAAATSYSSHRFTLIPFYIYYSMFGFQ
                     RVGDLAWAAGDIQARGFLLGGTSGRTTLNGEGLQHQDGHSHVLASTIPNCVTYDPCYA
                     YELAVIVHDGLKRMVENGENVFFYITVMNESYRQPAMPEGVEEGILRGIYRVRTVGDG
                     ETRRVRLLGSGTILREVEAAADMLHEDYGVSAEVFSVTSYNELARDGQDVERWNLLHP
                     EEPPRVSYVSQQLAGDDPVIASSDYMKSLAEQVRPYIGAPYKVLGTDGFGRSDTRESL
                     RHFFEVDRKFVALAALRSLADAQKIPLEEVRQARDAMGIDADKPNPRTA"
BASE COUNT          956 a         1954 c         1760 g          946 t
ORIGIN      
        1 tgacgcgaag tattccagcg ccgtcatcac caaagctttg accgacgaag ggttcagcaa
       61 cgtgcgctgc gtgaacgacg gcttcgccgc gttggacacg ctggaaaacc gttcggccca
      121 gatcctcatc gccgactggg tgatgcctgc catggacggg ctggagctga cccgccgggt
      181 gaaacgcctg gacgaggaga acggccactt cacgtacgtg atgctgctca cggggaggga
      241 agatttcgaa ggcatgctgg aagctttcga caccggggtg gacgactttc tcaacaagca
      301 caacctgcgc gccgagctgc agcccagaat catagccgcc cagcgcatcg ccgcgcgcca
      361 gaacgagctg ctgcgcacca acaagctcct gcgaaagaag gtacgcgagc tcagcgtggt
      421 cgatctcatc gacccggtga ccggcctggg caaccgccgc ttcaccatcg agcgcctcac
      481 agccgccatg gagcaggcgg aaagccgccg aatggcggca tgccttctac tcgtgggcgt
      541 cgacaacgtc gcggcgctca cggaaaggta cggtccgccc gcggtggacg agctgatgag
      601 cggcatcggg tcgaagatcc gcaccctcgt gcggcccctg gacctggtca ccaggcccga
      661 gcccggcatc ttcgcggtga tcacgctgca cccgagcatc gatgagtgca cgtccagcac
      721 ctttcagcgc atcttcgacg ggctctatct gaactctttc aagaccagcg aggcctacat
      781 tcccgtctcc gtcgggatat cgattaccgc cgctgacgcc cagaccggct ttccccatcc
      841 gcgccagctc atgcgccagg cggtgcacgc cctgggcgaa tcgttcgagt ccggccagat
      901 cgaggtccag cgggcggcga ccctgaacga gcccgtgacc gccgcagagc tctgagccgg
      961 cacgcaccca tgacacgaga gcgcaagcgg ataacccagg tgaccacgaa ggccggcgac
     1021 accggcaaaa cgtcgctggc ggacggctct aagctcgcca agacagaacc ccgcatgcac
     1081 gcgatcggca ctgtcgacga gctgaacagc ttcatcgggc tgctcgtcac gcagctcgac
     1141 cccgacgctg cgctgcgcga gccgtgcgcc agaatccagc aggagctctt cgacatcggc
     1201 gccacgctgg caaccgtggg agcgatcccc tgtcccgaca atgcctggat cgacgaggag
     1261 gttcagacgc tgaatgcccg cctgccgccc cttaccgagt tcgtcatccc cggcggcacg
     1321 gtcgcggccg ctctggccca cgtctgcagg acgacgtgcc gccgcgccga gcgcgagctg
     1381 tgggccttgc aggacgacga ttcggcgaca gcggccgccc gctataccaa ccggctgagc
     1441 gatctgctgt tcgtgatggc ccgcaccctg aacgccgccg agggagccga gcctcagtgg
     1501 cgcggcacgc gcggcccgaa gggcccggag tagcgcaggg gcccgaaagg cctagagaga
     1561 aagccggcgg atgtcggcca gcagctcgca gagatggctg acgaacctgc ccgcctcggc
     1621 gccgttgatt gcccgatggt cgtagctcaa cgacagcggg agcaattttc tcggcacgaa
     1681 gctctcgccg tcccagaccg gccgcatggc gagccgggac acacccagga tgcccacctc
     1741 cggggcattg atgatgggcg tgaagccggt tccaccaatg gcccccaggc tcgaaacgct
     1801 gaagctcccg ccctgcatgt cgtccatgcc cagcttctgc tcgcgggccc tgcccgcgag
     1861 gtcggtaaca gcctcggaga gctcccagat gcccatctga tccgcgcctt tgataaccgg
     1921 aacgaccagg ccctcctcgg tgtcgacggc gaagccgatg ttgtaataac cctttagaac
     1981 gtagttgcgg gcttcagcgt ccagggaggc gttgaaggtc gggaacttct tcagcgcgta
     2041 gcagcaggcc ttgatgatga atgccagcgg cgtgatcttc acgccccgcc gctccgcctc
     2101 gctcttcaga gaggcgcgaa aggcctcgag ctccgtgacg tccgcctcgt cgtgctgggt
     2161 gacgtggggc aggttcaacc agctgcgctg caggttggcg gcgcccgccg cgcgaatccg
     2221 cgtcagcggc cggatctcca ccgggccgaa tctggaaaag tcgatctgcg gtatggccgg
     2281 gatgcccgct ccgctcgcgg cccctgctcc ggcaccgccc ggcgccgcgg tcatggcatc
     2341 cttgacgtac gccttgacgt catccttcac gatgcgctcc cgcgcgccgg tgcccctcac
     2401 ccgggcgaga tccaccccca gctcacgggc gagccggcgc acggctgggc cggcgtagat
     2461 gcgttcgccc agagccgtcc cggccgactc ttgctccgcc gccgcagtgt ccgggccggc
     2521 cgggggcgca gccgtcgttg agataggctg cggggccgct gcttcggcgc ccctgggaac
     2581 ctcgcctgct tcgacctcgg gccgatctgc cgcaggtgcg ggggccgccg acgatccctt
     2641 aacctcgacc gtggcgatga gggtcccttc ggtaactgcc gcaccttccg atacgtgcat
     2701 ttccagaacc gttccggaac accctgccgg tatctccatg gacgccttgt cggactccac
     2761 cacgaccaga agatcgtcct cgccaacctg ctgcccggcg gcggctgcaa cctccaccac
     2821 cacgacgtcc tcggcctcgc cgatgtccgg cacccggatc tcgacggtac cggacgggcc
     2881 agcctcgggc tttgtcggcg gcgaggcgac ggcccgttcc gggggcactt tcggatcctg
     2941 catttgcggg ggcgcctcag cggccacctc gccggcctga tcctcgaccg gcgccgcccc
     3001 ggcagcctct atgacggcaa tgagatcgcc ctcggccacg gtgtcgccca cggccaccgc
     3061 cagggaagcc accacgcccg ccacccctgc cggcacctcc atggacgcct tgtcggactc
     3121 gatgatgatg agcgcgtcgt ccgcggccac ctcggcaccc gctgcgacgc agatctccac
     3181 cacctccacg tgctccacat cgccgatgtc gggaatgcgt atctctgtgg ccaactccag
     3241 gctccctcag gctgtccgcg gattgggttt gtcggcatcg atgcccatgg catccctggc
     3301 ctggcgaacc tcttcgagcg ggatcttctg ggcatccgcc aggctgcgca gcgcggccag
     3361 cgcgacgaac ttgcggtcga cctcgaagaa gtgtctgagc gattccctgg tatcgctgcg
     3421 tccgaaccca tccgtgccga gcaccttgta cggcgccccg atgtagggcc ggacctgctc
     3481 ggccagggac ttcatgtaat cgctggaggc gatcaccgga tcgtctccgg cgagctgctg
     3541 cgagacgtag ctcacccgcg gcggctcttc cggatgcagc aggttccaac gctccacgtc
     3601 ctggccgtcg cgagccagct cgttgtagct cgtgacgctg aacacctcgg cgctgacgcc
     3661 gtagtcctca tgcagcatat cggcagccgc ttcgacctcg cgcagtatgg ttccgctgcc
     3721 gagcaggcgt acccgccgcg tttcgccatc gccaaccgtg cgcacccggt agatgccccg
     3781 caggatgccc tcctccaccc cttcgggcat ggccggctgc cgatagctct cgttcatcac
     3841 cgtgatgtag aagaaaacgt tctcgccgtt ctcgaccatc cgcttcagac catcgtgaac
     3901 gatcacggcc agctcgtacg cgtagcaggg gtcataggtg acgcagttgg ggatcgtcga
     3961 ggcgagcacg tggctgtgac cgtcctgatg ctgcagcccc tcgccgttga gcgtcgtccg
     4021 tcccgacgta cccccgagca gaaaaccgcg cgcctgaatg tccccagcgg cccaggccag
     4081 atcgcccacc cgctgaaaac cgaacatgga atagtagatg tagaagggaa tcagcgtgaa
     4141 gcggtgcgag ctgtaggacg tggcggccgc caaccaggcc gagaaagcgc cggcttcgtt
     4201 gatgccctcc tcgagcacct ggccgtcccg ggactcgcga taggcggcaa tctctccgta
     4261 atcctcgggc tcatatagct gcccggccga ggagtagatg cccagctggc ggaacatgcc
     4321 ctccatcccg aacgtgcggg cctcgtcggg aacgatggga acgattcgct tgccgatgtt
     4381 cttgtcgcga accagggtcc ccaacatgcg cacgaacgcc atggtggtcg agttggaccg
     4441 gtctccggtt ccctgcagct gctgcgcgaa ggcgctgagc tccggaacct gcagcgcttc
     4501 cgatgccgtc gtgcgctggg gaatgggacc gccgaggtcg gcccgtttct tgcgcaggta
     4561 caccatctcc gggctgtcct ttggaggacg gtagaacggg atgtcctcca gctccgtatc
     4621 agaaagcggg acgtcgaagc gatcgcggaa acgcttgagc tcgtccaggt tgagcttctt
     4681 cacctggtgg gtgtcgtttt ccgcctcgcc agcgtcgccc atgccgtatc ccttgacggt
     4741 cttcgccagg atcaccgtag gctcgtcttt gtgatgcacg gcctcgtgat aggccgcgta
     4801 caccttgaag gggtcgtggc cgccccggtt gagccgcatg atgtcatcgt ccgagaggcg
     4861 ctcgaccatg gccaggagct ccggatgcgc gccgaagaag tgttcgcggg tgtaggcccc
     4921 gcccttggct ttgtaattct ggtactcgcc gtccaccacc tcgttcatgc gcagctgcat
     4981 caggcccgcg gtgtcgttgg cgaacagcgg atcccagagc cgaccccaca ccaccttgat
     5041 cacgttccag cccgcgccgc ggaagttacc ttccagctcc tggatgatct tgctgttgcc
     5101 ccgcacagga ccgtccagcc gctgcaggtt gcagttgacg acgaagatca gattgtcgag
     5161 cttctcggtg ccggccttgg acagggcgcc gagcgcttcg ggctcgtcgc actcgccgtc
     5221 gccgagaaag gcccacacct tgcgctcgcc catgggcaga agcccccggg catccaggta
     5281 cttcatgatg tgcgcctggt agatggcctg gatcggcccc agccccatgg agaccgtggg
     5341 aaactgccag tagtcgggca tcagccaggg gtgcggatag gatgagagcc cctcccctgc
     5401 cacttcccgg cgaaagttgt ccagctgggc ctcgctcagg cggccctcga gaaacgaccg
     5461 ggcgtatatg cccggcgagg cgtgcccctg aaagtagatc aggtcgcccg ggtgctcgtc
     5521 cgtcgggccc cggaagaaat agttgaagcc cacgtcgtaa agcgtggccg acgaggcgaa
     5581 gctggagatg tgcccgccca gatcgcccgg acgccg
//
LOCUS       DBNHLM010000317         8999 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1544246, whole genome shotgun sequence.
ACCESSION   DBNHLM010000317 DBNHLM010000000
VERSION     DBNHLM010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8999)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8999)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8999
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1544246"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="AC16QF_14490"
     CDS             <1..676
                     /locus_tag="AC16QF_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449817.1"
                     /translation="AVAPSVLESYACSYKPGKDREDLDKLRDFYLKQAEKAGIAAPPA
                     WLWTLNKGAAPVNFVWLNVHESLTAFGGTDDAIAASPEMAEAFERADTVMTCQAGLGT
                     FRQTYGGEMQESDSSVVSFGCGFREGSGPATMDALHAQIADLNEMAGDLAVNSIFEVT
                     PLTQGPGGSDIVMVVITDGTSGWARHMDYINSIPAGLAAQSHFNAALDCNMNLWRLEQ
                     IVGGGD"
     gene            complement(725..1033)
                     /locus_tag="AC16QF_14495"
     CDS             complement(725..1033)
                     /locus_tag="AC16QF_14495"
                     /inference="COORDINATES: protein motif:HMM:NF012267.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HIR6449818.1"
                     /translation="MTHVVTEACIGVKGEGCMEVCPEFCILSEPADHMSYIDPARCTD
                     CGVCVAACVVGAIFADKDVPRASREFININESWFRHKTGVRRRVRAMASEIGHWLPPE
                     "
     gene            1183..2055
                     /locus_tag="AC16QF_14500"
     CDS             1183..2055
                     /locus_tag="AC16QF_14500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6449819.1"
                     /translation="MKVGLAFASSIGIDGATALDICRRAEAAGFESVWGGEHVIIPDA
                     IASKYPYTPDGKVPVEADTPIPDPLMWLAFAAAAAPTLRLGTCILIVPQRNPLILAKE
                     LATLDQLSGGRVELGLGVGWLKEEFEALGVPWERRGARNDEYIAAMRALWAGPHAEYH
                     GDFVDFAPVTCSPRPVNGSIPVIVGGDTDAAIARAVRLADGYFPGEGDAERLGALLGR
                     LRAAAEQAGRDPASIEVNAMFGVQMADPAAGIEQMAGLGVDRIMIPAFFFAGPDGLDR
                     LSEFGERVMPLTRG"
     gene            complement(2072..3088)
                     /locus_tag="AC16QF_14505"
     CDS             complement(2072..3088)
                     /locus_tag="AC16QF_14505"
                     /inference="COORDINATES: protein motif:HMM:NF016241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HIR6449820.1"
                     /translation="MRHFPLLIAAMLTGILSSHAHATDFVMMKNGDRITGEIKQIWNG
                     EVFIEPEYGDTYPIDLDYVAYVHTDEDFEVEFVEGRRSRTVTGRLGLDDLGRPSVIVG
                     DAAETYPLAKIDNMQEIEDFFEWELATDISVNLATGNSDTRSSRFNVVGKMKLGEHRH
                     RLEYTRDQASAEGDTTKDQTYVAYEDVWTFRDDWFVRGAVNWTRDPIRDLASRSQIFL
                     GPGFHFWDDSKRTLNLSVGPNYLSEDIGGENKQSVAAQLVFRYEQHFLDDDLVVFQET
                     DYQTIVSGRKNKILNTSTGVRWDVLEDVYIKMQVDFDYETNPASAREKEDVTYLVGVG
                     LELD"
     gene            complement(3208..4272)
                     /locus_tag="AC16QF_14510"
     CDS             complement(3208..4272)
                     /locus_tag="AC16QF_14510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6449821.1"
                     /translation="MKAETLLPLGKLDPGLAAPEAALDLHAVAEDARTVDALGYWGLL
                     MEETKDDPFQVLAMAAAATSRVRLGTSVAIAFARSPFVTAMSAWTLQKLSNGRFELGL
                     GSQVRGHIRRRFGLQWHPPGPWMRDYVRAVRAIWASWQNGTKLDFSSQHYEIDLTVPL
                     FTPTPIDHPDIPIFVAAVNAYMCGVAAEVADGVRLHPVCSPRYIRDVVAPAVAEKRPA
                     GKPFEVCLKPLIAAADDGKTLSERAEVARQRLAFYVSTPAYRRPFENAGLGDLAAELS
                     RLSKEQRWHAMAERISDDVLEEWAVVATYDELAATIRSRFDGLVDRVEVGIPVASPED
                     RESLSRIVAELQSGGGSRAS"
     gene            complement(4435..4668)
                     /locus_tag="AC16QF_14515"
     CDS             complement(4435..4668)
                     /locus_tag="AC16QF_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449822.1"
                     /translation="MGIQDLGAIGEFISSLVIVVTLLVLIYEVRGAKNATLQANAQER
                     QPKLDAVDTDLSRQSHQSTSEVYKSGWRGCSDY"
     gene            complement(4822..5280)
                     /locus_tag="AC16QF_14520"
     CDS             complement(4822..5280)
                     /locus_tag="AC16QF_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449823.1"
                     /translation="MDHQAFAEMLGNYGEFLGSVAVLATLLYLAAQIRQNSALAKAQL
                     ETDVMQAFAGAADFPNQHPEVIAKLRAGVDLDDVETVLRDGFVLNYLFACAIGHQASK
                     YADPDRTQRFVMSAALTLGNVDMLIEEHETRLRTSGYEEFVNRVKLELAN"
     gene            complement(5551..6528)
                     /locus_tag="AC16QF_14525"
     CDS             complement(5551..6528)
                     /locus_tag="AC16QF_14525"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HIR6449824.1"
                     /translation="MKICIFGAGAIGGYLGARLAATDVDVSLVARGPHLEAMRRDGLT
                     LREAAEETVVAVNATDDPATLGRQDYVVVTLKAHSVPAIVERLPPLLGPSTAVVTASN
                     GVPWWYFHGLEGPHANRRLRSVDPDDVQWNGIGPERAIGCVVYPACEIEAPGVVRHTE
                     GNRFVLGEPSGEKSERVSALSEAFIDAGLRAPIRRIRDEIWMKLWGNLCFNPISALTT
                     ATLDVIATDSPGTREVAAAMMREAQTIGERLGVRFGVDMDKRIAGAAAVGAHRTSMLQ
                     DLELGRPMEIDALLTAVQEMGEITGVATPTIDVVLALVRQRARIAGCYG"
     gene            complement(6525..8042)
                     /locus_tag="AC16QF_14530"
     CDS             complement(6525..8042)
                     /locus_tag="AC16QF_14530"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HIR6449825.1"
                     /translation="MNTIRDLLASGMPDAPAIAAPDSEPCTFADLMDQVERTVGQLNA
                     FGVTTADRAAIVLPNGPEMAAAFVSIACGTTTAPLNPGYREDEFEFYLSDLDPKVLVV
                     ETGSVSPARAVAERLDIRTLEIAPHSSGRAGLFDFADAPVPVTPGYGTEDDVALMLHT
                     SGTTSRPKLVPLSHRNLSTSATNIQATLALTPADRCMNIMPLFHIHGLAAAILASLGA
                     GGSTYCTPGFNALRFFAWLEDAQPTWYTAVPTMHQAILGRTAGNRAAIDAHPLRFIRS
                     CSAALPPQIMQAVEEAFGCPVIEAYAMTEAAHQMTSNPLPPAPRYAGSVGVAAGPEVA
                     IMDDAGNLLGPGETGEVVIKGPNVTSGYIANPKANAEAFHHGWFRTGDQGVMDDGGYL
                     RITGRLKEIINRGGEKIAPREIDEIILEHPAVDQIVTFAMPHDKLGEDVAAAVVLAEG
                     ASADAAEIRAFAAERLADFKVPRKVVFVTEIPKGPTGKLQRIGLAEQLAEALSRS"
     gene            8155..8277
                     /locus_tag="AC16QF_14535"
                     /pseudo
     CDS             8155..8277
                     /locus_tag="AC16QF_14535"
                     /inference="COORDINATES: protein motif:HMM:NF017470.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8410..8862)
                     /locus_tag="AC16QF_14540"
     CDS             complement(8410..8862)
                     /locus_tag="AC16QF_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449826.1"
                     /translation="MGIQDLGAIGEFISSLVIVVTLMVLIYEVRGAKNATLQANAQER
                     QRKLDDYLRSVAESPDLAGIIETANQHLGGARLADPATEFGLERDQYARLYYQNLRVL
                     TQARDAYVSSLPEDERLALDALIAANLSVPSQACRRHRPLPTVPPGNV"
BASE COUNT         1570 a         2827 c         2900 g         1702 t
ORIGIN      
        1 ggcggtggcg ccgagtgttc tggaaagcta tgcctgcagc tacaagccag ggaaagacag
       61 ggaagatctg gacaagctga gggacttcta tctcaagcag gcggaaaagg ccggcattgc
      121 cgcgccgcct gcatggttgt ggaccctcaa caagggcgct gcaccggtaa acttcgtttg
      181 gttgaacgtg catgaaagcc ttaccgcttt cggggggacc gatgatgcca tcgccgcatc
      241 gccggagatg gcggaagctt tcgagcgggc agataccgtc atgacctgtc aagctggact
      301 gggaacgttc aggcagacgt atggtggaga gatgcaggaa agtgacagca gcgttgtttc
      361 attcgggtgc ggattcagag agggatccgg tccggcgacc atggatgccc ttcacgccca
      421 gatcgctgat ctgaatgaga tggccggcga tctggcggtc aactcgatct ttgaagtcac
      481 gccgctgacg cagggaccgg gcggatccga catcgtgatg gtggtaatca cggacggcac
      541 gtccggatgg gcccgtcaca tggactacat caactccata cctgccgggc tcgcagccca
      601 gagccacttc aatgccgctc tggattgcaa catgaacctc tggcgtctcg agcagatcgt
      661 cggcggcgga gattaatctc gacgcggggc cgaccccatg cgggtcggct cctcgcggag
      721 cgcctcattc cggcggcagc cagtggccaa tttcggaagc cattgctctg acgcgcctgc
      781 gcacgcccgt cttgtggcga aaccaggatt cgttgatgtt gatgaactcc cgcgatgctc
      841 tgggaacgtc cttgtcggcg aatatggcgc cgaccacgca ggcggcgacg cagaccccgc
      901 agtccgtgca gcgggcggga tcgatgtacg acatgtgatc ggccggctcg ctgaggatgc
      961 agaactccgg gcacacctcc atgcacccct cgcccttgac gccgatgcag gcttcagtca
     1021 cgacgtgggt catcaattct gttccgttcg tctgcggtca gccgatctta ccccaagccc
     1081 ggggctaccc tgcgggtttc cgatcgggac cccgaagaga ttgggtcctg gcgaacgagg
     1141 cggtgtagga tgtgcctttc aaaccagcag gggaatcaaa cggtgaaagt cggactagcc
     1201 ttcgcgagca gcatcggcat cgatggcgct actgctctgg atatctgccg ccgagccgag
     1261 gcggcgggat tcgagtcggt ttggggcggt gagcacgtca tcatccccga tgccatcgct
     1321 tccaaatatc cctacacgcc ggacggcaag gttccggtgg aggcggatac cccgatcccc
     1381 gaccccctca tgtggctggc ttttgcagcc gcggcggcgc ccacgttgcg tctcggcacc
     1441 tgcattctga tcgtgcccca gcgcaacccc ctgattctcg cgaaggagct ggcgacgctg
     1501 gaccagctct ccggcggccg ggtcgagctt ggtctcggcg tcgggtggct gaaggaggag
     1561 ttcgaagccc tgggcgtgcc ctgggagcgg cgcggagcgc gcaacgacga gtacatcgcg
     1621 gccatgcgcg cgctttgggc cggaccacat gcggagtatc acggggattt cgtggatttc
     1681 gcgcccgtga cctgcagccc gcgtcccgtg aacggctcga ttccggtgat cgtcggcggc
     1741 gacaccgatg cggccatcgc tcgcgcggtg cgcctggcgg acggctattt ccctggcgag
     1801 ggggacgcgg agcggctcgg tgccttgctg ggacggctgc gtgccgcggc agagcaggcg
     1861 ggtcgggacc ccgccagcat cgaggtcaac gccatgttcg gcgtgcagat ggcagacccg
     1921 gcggccggca tcgagcagat ggcggggctg ggcgtggaca gaatcatgat tccggccttc
     1981 ttctttgccg gaccggatgg tcttgaccgt ctcagcgagt tcggcgagcg ggtgatgccc
     2041 ctgacccggg gttgagcgcg cctcagcgga ctcaatcgag ctccagcccg acgccgacca
     2101 gataggtgac gtcttctttc tcgcgggcgc tcgccgggtt ggtctcgtag tcgaagtcca
     2161 cctgcatctt gatgtagacg tcttcgagca cgtcccatcg cacgcccgtg ctggtgttga
     2221 gaatcttgtt cttccggccg ctcacgatgg tctggtagtc ggtttcctga aaaacgacga
     2281 gatcgtcatc caggaagtgc tgctcgtagc ggaagaccag ctgagctgcc acggattgtt
     2341 tgttctcccc gccgatgtcc tccgagaggt agttggggcc gacgctgagg ttcagcgtgc
     2401 gcttggagtc gtcccagaag tggaagccgg ggccgaggaa gatctggctt cggctcgcca
     2461 ggtcccgaat cgggtcgcgc gtccagttga cggcgccgcg gacgaaccag tcgtcgcgga
     2521 acgtccagac gtcctcatag gcaacgtatg tctggtcctt ggtagtgtcg ccttcggcgc
     2581 tcgcctggtc ccgggtgtat tcgagccggt gtcgatgctc ccccagcttc atttttccca
     2641 ccacgttgaa gcgggaggag cgggtgtccg agtttccggt tgcgaggttg acgctgatgt
     2701 cggtcgccaa ttcccattcg aagaagtcct cgatttcctg catgttgtct atcttggcca
     2761 gcgggtaggt ctccgccgca tcgccgacga tgacggacgg acggcccaaa tcgtccagcc
     2821 ccagccgtcc cgtcaccgtg cggctgcgcc ggccctcgac gaactcgacc tcgaagtctt
     2881 cgtccgtgtg cacgtaagcg acgtagtcga ggtcgatggg atacgtatcg ccgtactcgg
     2941 gctcgatgaa gacctcgccg ttccagatct gttttatctc gcccgtgatt cggtcgccgt
     3001 tcttcatcat gacgaagtcg gtcgcgtggg catggctgct cagaatgccg gtaagcattg
     3061 ctgcgatgag caatgggaaa tgtcgcatgt cgggtcctcg agctcgacga attcagcggc
     3121 gcgatgagtg gcgcgctctt caggggcggg tgttctgcca caattcgctg gaaaggtcca
     3181 attctcgtgc ggtggacgag ccccggctca gctcgcgcgg ctcccgccac cgctttgcaa
     3241 ctcggcgacg atcctcgaca gcgactccct gtcctcgggg cttgcgaccg ggatgccgac
     3301 ctccaccctg tcaacgaggc cgtcgaagcg ggaccgaatc gttgcggcga gctcgtcgta
     3361 ggtggcgacc accgcccact cctccagcac gtcgtcgctt atgcgctcgg ccatcgcatg
     3421 ccatcgctgt tccttagaca ggcggctgag ctcggcagcg aggtcgccca ggccggcgtt
     3481 ctcgaacgga cggcggtatg cgggtgtgct cacgtaaaag gccaggcgct gccgggcgac
     3541 ctctgcccgc tcgctgagcg tcttcccgtc gtcggcggcc gcgatcagcg gcttcaggca
     3601 gacttcgaac ggcttgcccg cgggccgttt ctcggccacg gcgggtgcca ccacgtcccg
     3661 gatgtagcgg ggcgagcaga cgggatgcag gcgcaccccg tcggccacct ccgcggcgac
     3721 gccacacatg taggcgttga ctgcggcaac gaatatggga atgtcgggat gatcgatggg
     3781 ggtcggcgta aaaaggggta cggtcaggtc gatttcgtaa tgttggctcg aaaagtcgag
     3841 cttcgtgccg ttctgccagc tcgcccagat ggctcgcacc gccctcacgt agtcgcgcat
     3901 ccacggtccc ggaggatgcc actgcaggcc gaagcgtctg cggatgtggc cgcggacctg
     3961 agaacccagg ccgagctcga agcggccgtt ggacagtttc tgcaatgtcc acgccgacat
     4021 ggcggtgacg aatggactgc gggcgaaggc gatggccacg gaggtgccca ggcgcacgcg
     4081 gctggttgcc gcggccgcca tggcgagcac ctggaacgga tcgtccttgg tttcctccat
     4141 gagaagcccc cagtagccga gcgcgtccac ggtgcgggca tcttctgcaa cggcgtggag
     4201 gtcgagcgct gcctcgggcg ccgcgagccc tggatccagc ttgcccagcg gcagaagggt
     4261 ctcggctttc atgtcggttt ccccgtcccg gtcgacgtgg tgaaggcggg gagagtctaa
     4321 cgaaccggtg gggggtcgtc gaaacgccct gattggctga tccttcccgc ctggcgccgg
     4381 cggtaatacg cgcccgcccg tgatctcccc ccatctggcg ggtttgctct tacctcaata
     4441 gtctgaacaa cctcgccacc cgcttttgta gacttcagaa gttgactggt gggactgccg
     4501 ggagagatcg gtatctacgg cgtctagctt gggctgccgc tcctgtgcgt tagcttgaag
     4561 agtcgcgttc ttcgcgccgc gtacctcgta tatcagcacc aagagcgtga cgacgatgac
     4621 gagcgagctg atgaactccc cgatggcccc gagatcctga atacccattg gacgtctccg
     4681 gttgtgggtg acggggttta gtccaacgcc agcagtggtc ggatcaactt agcttcaatg
     4741 cgaccggcgc tgtttcaatt tcgggcaact catcaagata ggcgcgcacc cgctgaccgg
     4801 ttgcggcatc tgctgatgtc gtcagttggc cagttcgagc ttcactcggt tgacgaactc
     4861 ctcgtaccct gatgtgcgaa ggcgagtctc atgctcctca atcagcatat cgacgttccc
     4921 cagtgtaagc gccgcactca ttacgaaccg ttgggtacgg tccggatcag cgtacttcga
     4981 agcctgatga ccgatggcgc aggcaaatag gtaattcaac acgaagccgt cccgcaaaac
     5041 ggtctctacg tcgtccagat caacgccggc ccgcagcttg gcaatgacct ccggatgttg
     5101 gttcgggaag tccgcggccc cagcaaacgc ctgcatgaca tcggtctcga gttgtgcttt
     5161 cgccagtgca gagttctgtc tgatctgcgc cgcgagatag aggagcgtcg ctaagactgc
     5221 gaccgagccc agaaactccc cgtagttgcc cagcatctcc gcaaaggcct gatggtccat
     5281 gtcggtctcc tctccaatga ttggaacgag gctacctcag ccgatctgcg gggcaagtct
     5341 tctgcgccaa catccttcca tgcgcaggcg ccggtgagac gtcgactgga acgtgatcgg
     5401 tgcaatcggg gagcctatcg gggcagccct tttccttatc tcaatgcttt atcttgccct
     5461 gcaggtgaag acggcaaagc agatgatggc tgtaaaaacc gctcggcgtt tccgacttcc
     5521 cggaagaacg ggcggggtcc gggacagcgc tcagccgtag cagccggcta tccgcgcccg
     5581 ttgtctgacc agagccagaa cgacgtcgat ggtcggcgtt gctacccccg tgatctcgcc
     5641 catctcttgg accgccgtca gcagcgcgtc gatctccatc ggccggccaa gctcaagatc
     5701 ctgcagcatg gaggtgcggt gtgcgcccac ggcggcggca cccgcgattc gcttgtccat
     5761 gtccacgccg aacctcactc cgagtcgctc gccgatggtc tgcgcctcac gcatcatggc
     5821 ggccgcaacc tcccgggtcc cgggcgagtc ggtcgcgatg acgtccagcg tagccgtcgt
     5881 cagggcgctg atggggttga agcagagatt gccccaaagc ttcatccaga tctcgtcgcg
     5941 gatccgacgg atcggagcgc gaaggccggc gtcaatgaat gcctcgctga gcgcggacac
     6001 ccgctcggat ttctcgccgc tgggctcgcc gagtacgaag cgattgcctt cggtgtggcg
     6061 gaccaccccg ggtgcctcga tctcgcaggc cggatagacg acgcagccga tggcccgttc
     6121 cgggccgatg ccgttccact gcacgtcatc gggatcgacg ctcctgagac ggcggttggc
     6181 atgcggacct tcaaggccgt gaaaatacca ccagggtacg ccgttgctgg ccgttaccac
     6241 agccgtggac gggccgagca gcggcggcaa ccgttcgacg atcgccggca ccgagtgcgc
     6301 cttgagcgtg acgacgacgt agtcctgacg gccgagggtc gccggatcgt ccgtggcgtt
     6361 gaccgccacg acggtctcct ccgcagcctc ccgcagcgtc aacccatctc gccgcatggc
     6421 ctccagatgc ggcccacgcg cgacaagcga cacgtcgacg tcggtcgcgg cgagccgtgc
     6481 gccgagatat ccgccaatgg cgcccgctcc gaagatacag atcttcacga ccgcgaaagc
     6541 gcttcggcaa gctgttcggc cagcccgatc cgctgcagct tgccggtcgg acctttcggg
     6601 atctcagtga cgaaaaccac cttgcgcggc accttgaagt cggcgagccg ctcggcggcg
     6661 aacgcacgaa tttccgccgc gtcagcactc gcaccttccg caagcaccac cgcggccgcg
     6721 acgtcttcgc cgagcttgtc gtgcggcatg gcgaaggtaa cgatctggtc tactgcggga
     6781 tgttctagga tgatctcgtc gatctcacgg ggcgcgatct tctcaccgcc gcgattgatg
     6841 atctccttca ggcgtcccgt gatccggaga tatccgccgt cgtccatcac gccctgatcg
     6901 ccggtccgga accagccgtg gtggaaggcc tccgcgttgg ccttcgggtt ggcaatgtag
     6961 ccggacgtga cgttcgggcc ctttatgaca acctcgccgg tctcgcccgg cccgagcaga
     7021 ttcccggcat catccatgat cgcgacctcc gggcccgccg ccacgccgac gctgcccgca
     7081 tagcgcgggg ccggcggcaa ggggttgctg gtcatctggt gcgccgcctc cgtcatggcg
     7141 taggcctcga tgaccgggca gccaaacgcc tcctcgaccg cctgcatgat ctggggcggc
     7201 aacgccgcgg agcatgagcg gatgaacctt agcggatgag catcgatcgc tgctcgatta
     7261 ccggccgttc gacccaggat cgcctggtgc atggtcggca ccgcggtgta ccaggtcggc
     7321 tgcgcatcct cgagccaggc gaaaaaccgc agggcgttga agccgggtgt gcagtaggtc
     7381 gagccgccgg cacccaggga ggccagaatg gcggcggcga gcccgtggat gtgaaagagc
     7441 ggcatgatgt tcatgcaccg gtccgccggt gtcagcgcga gcgtcgcctg aatgttagtc
     7501 gccgaggtcg acaggttgcg atgggacagc ggcacgagct tggggcgcga cgtcgtgccc
     7561 gaggtgtgca acatcagcgc aacgtcgtcc tcggttccgt agcccggcgt gacgggaacc
     7621 ggcgcgtccg caaagtcgaa aagcccggca cgaccgctcg agtgcggcgc aatctcgagc
     7681 gtgcggatat cgagtcgctc cgcgacggcg cgagccgggg acaccgagcc ggtctcgacc
     7741 acgagcacct tagggtcgag atcggagagg tagaactcga actcgtcctc ccgatagccg
     7801 ggattgagcg gcgccgtcgt cgtgccacac gcgatgctga cgaatgccgc cgccatctcc
     7861 ggcccattcg gcaggactat ggcggcgcga tccgccgtgg tcacgccaaa cgcattgagc
     7921 tgccccacgg tccgctcgac ctgatccatc aggtcggcaa aggtgcaggg ctccgagtcc
     7981 ggcgcggcga tggcaggcgc atcgggcatt ccgctggcga gcaggtcgcg aatggtattc
     8041 atgaacatcc tcggatcgtg gggcgcctat tctgcctccc tctccggaca atgttaacgc
     8101 gccgcacgaa agaccggtct ctgctctcga actgccgaac ggcaaagcgg ctggtggtgc
     8161 ctgatcatcc tgatgccgat tgtcggcgtt atcgtcctgc tgtttttctt ggtcatgagg
     8221 ggtacggacg gcaccgatcg cttcggcggc gatccgaaga cctcacccga gccatagata
     8281 cgcactgcaa tcggtgggcg gcacagtagt tgggccacaa acggaccacg tccagggcac
     8341 ttcgctggcc aatacggcca gcggatcaag cggtagccaa acaaatcgcg ccacctgcca
     8401 ctgtggactt cagacgttgc cgggtgggac tgtcgggaga ggtctatgtc gtctgcatgc
     8461 ttgcgatgga acactgagat tggccgcaat cagcgcgtcc agagccagtc tctcgtcctc
     8521 gggcaggctg gaaacatagg cgtcccgagc ctgagtcaac acccggaggt tttgatagta
     8581 caaacgtgca tattgatcgc gctctagtcc gaattccgtc gccggatcag ccaaccgggc
     8641 cccgccgagg tgctgattcg cggtttcgat gatgcccgcc agatcgggcg attccgccac
     8701 ggaacgcagg tagtcgtcga gcttacgctg ccgctcctgc gcattggctt gcagggtcgc
     8761 attcttcgca ccccgtacct cgtagatcag taccatcagc gtgacgacga tgacgagcga
     8821 gctgatgaac tccccgatgg ccccgagatc ctgaataccc attggacgtc tcccgtagtg
     8881 gatgtcggga gggtagtcca agctcgccgt caacagtccg agagggtgga gttttgctgc
     8941 gctatcgccc gttcaactct cggaggtagc ggaatgccca atcgtcggca gcagggacc
//
LOCUS       DBNHLM010000318         7705 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1125581, whole genome shotgun sequence.
ACCESSION   DBNHLM010000318 DBNHLM010000000
VERSION     DBNHLM010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7705
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1125581"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="AC16QF_14545"
     CDS             complement(<1..378)
                     /locus_tag="AC16QF_14545"
                     /inference="COORDINATES: protein motif:HMM:NF015684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR6449827.1"
                     /translation="MIINRYLVRELLLPFFAVGGVLTVIFTTYSMTRFLDKAADGLLR
                     TSEVLQLTALKSFIAQEVLLPVSLFVALVLALGRLYSDWEMTALRSFGFAERQVLFPI
                     MSVALGFALVVAVFSLVGRPWAYE"
     gene            468..977
                     /locus_tag="AC16QF_14550"
     CDS             468..977
                     /locus_tag="AC16QF_14550"
                     /inference="COORDINATES: protein motif:HMM:NF015901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HIR6449828.1"
                     /translation="MLKRFFAAELASAIALALVQLSCSAWAGELPALDDQDTVVVTAD
                     QAWESEDGDSLSFAGNFNLNAPDFHVASDTAVIHGPLEDPNRIVAEGSPVRFWVADPD
                     SGNRTHGTGRRLEYDRQRDLVRLEGDAVLEDDRTVMRSSELEYNTATKRLVAQGSGGV
                     EIVTNPNRD"
     gene            complement(987..1415)
                     /locus_tag="AC16QF_14555"
     CDS             complement(987..1415)
                     /locus_tag="AC16QF_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR6449829.1"
                     /translation="MNATAEKFGYPDTLVREYGHWLVLVRPQQVSLGSLVLVCKEPVT
                     RFSEVSRAAFTELHDVIAAAEGALSSLYSYDRINYLMLMMVDPDVHYHVIPRYEAARL
                     LGTVEIADHGWPGPPALGEFHELDAAEREALLGQLREAWS"
     gene            complement(1417..2169)
                     /locus_tag="AC16QF_14560"
     CDS             complement(1417..2169)
                     /locus_tag="AC16QF_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HIR6449830.1"
                     /translation="MKAIILSAGQGRRLLPLTAGTPKCGVEIAGQSLLEWQLGEVAQC
                     GIDEVVVVTGFHADIVDDLVSRCSMVPVRTLHNPFFDASDNLGTCWVARSEMTDPFVI
                     INGDTLFEAAILQKLLASPANAPVTLVVDRKDHYDEDDMKVVVSDGRLRRVGKRLELD
                     DVNGESIGMMAFRGLGPAKFVSKVESLMRSDMGLKLWYLSAVDQLAQSGEVGVCDIHG
                     LSWCEVDDRDDLDNAAGVVTAWRKPASRAAAD"
     gene            complement(2222..3832)
                     /locus_tag="AC16QF_14565"
     CDS             complement(2222..3832)
                     /locus_tag="AC16QF_14565"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6449831.1"
                     /translation="MLLDLEKLPQGERFQADVCIAGAGAAGVALARRLAELGHSICLL
                     ESGGLDFEQSTQDLYAGTSDGMTYYDLVDARLRFLGGTTNIWGGRCALLDDIDFRRRP
                     WVPHSGWPITNAELTPYYAAVHDELELGEVRYDESLWDEVGERVPPFDPDAVSTRFWR
                     FDDVRERFAARRVQDLASSAAVTLVLHANVTHIQAAANAASIEHLKVSSLSGIEGRVT
                     ARHYVLACGGIENARLLLASRDVEAAGVGNGNDQVGRYFMEHPHGRAGRVSGPGAFPL
                     WAAFRKRFRSHGPDLAPVLVPSAAAQEREGILNSAFTLSLQRDPERGVAVTRQMYQTL
                     KHGMAPTRRGRTLWHAYRAVRSSAQRLARVPFERLRAGLGMRRLYLMIRAEQAPNPDS
                     RVTLGSERDALGMPRAALSWQLGELDKRTIQVMVRLLDGELTRLGLGFVEASSWLGEP
                     GNDWPVDPTVGNHPIGGYHHIGTTRMSADPRHGVVDGSGRVHGYGNLFVAGSSVFATA
                     GWANPTLTILALALRLGDHLHGLLGGKS"
     gene            complement(3835..4575)
                     /locus_tag="AC16QF_14570"
     CDS             complement(3835..4575)
                     /locus_tag="AC16QF_14570"
                     /inference="COORDINATES: protein motif:HMM:NF016974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HIR6449832.1"
                     /translation="MSGTASLPRRWVIKGGKRLLRAIGRFQGRHSLVGTGPFLENAEF
                     PWVPSLEGRWEEIRSELATVMEHPEDIPAFHQLSPDQKRISKGDNWKTFAFYVFGIRL
                     EENCRRCPVTAATIECLPGLQNAWFSILAPRYHIPPHKGPTRAVIRCHLGLAVPRDGE
                     RCWLRVDDQIRHWEAGGCLLFDDTFEHEVRNDTDETRIVLFLDVDRPSDRLGTWFNRL
                     IVTLIRGSAYVKDPLKNLADWNRARREA"
     gene            complement(4572..5363)
                     /locus_tag="AC16QF_14575"
     CDS             complement(4572..5363)
                     /locus_tag="AC16QF_14575"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HIR6449833.1"
                     /translation="MSEVSVCGAVVLAGQRAGPTPLCAQAGVDVEVLVPVGGVPAIER
                     VLGALSRAELVRPAVVVGPSPEVLSAHPEVKAMIDAAGAAWVAPAGDPASSALAGLQG
                     QAPPTLVTSGDHALLCASIVDAFCEAAHRVQADLIVGLVPYQAVRAAYPESRRTVLKF
                     ADGARCGANLYFLRTEEARRGIEFWVAVQRDRKRPWRIARRLGWRLLLRYLTGRLTLG
                     AALGALSERAGCRLGSVIVDEPRAAVDVDSVDDWRLAQRIVGEAS"
     gene            complement(5360..6496)
                     /locus_tag="AC16QF_14580"
     CDS             complement(5360..6496)
                     /locus_tag="AC16QF_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017845642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HIR6449834.1"
                     /translation="MSVVPVSGRSGLNDFIEVPWVVYADDPCWVPPLKMERRDALSPK
                     HPFFEHARWQPFVAYRDGRAVGRISAQIDELYQQRHDPKGGFFGMFESIPDDAVIDGL
                     LASAEAWLAERGCEHVVGPFNLNINQEVGLLVDGFDSSPFFMMGHGMPYYGEALARRG
                     YAEVQRMLAYEMPVLFERPRLMSIMMKQLGPRVTMRTLDRRKLVEELEILRDIFNDAW
                     SENWKFVPWTSREFLAVGKEMMMLIPPDLVQIAEVDGEPAAFIVMLPNLNEVIGDLDG
                     RLLPFGWAKLLWRIKVRYPKSGRVPLMGVRRRFHRTRLGPGLAMFCISALREPAHRAG
                     LERAELSWILEDNQGMRSILEAVGGEVTRTYAMFEKSLSRGPGS"
     gene            complement(6516..7034)
                     /locus_tag="AC16QF_14585"
     CDS             complement(6516..7034)
                     /locus_tag="AC16QF_14585"
                     /inference="COORDINATES: protein motif:HMM:NF021438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HIR6449835.1"
                     /translation="MRELHYLRGPWAAAASILMLVTGVLWTPVASANQTVDTDAKASR
                     VEGVWASKGAVFAIERSGDTLQATVVALRKPRPDRKNAKAELRDRPLIGLQLLSDYRF
                     KRGRWRGDFYDPGSGRTYSSYIRLDDEGNLRLRAWVLMFGHTEVMEPVGNCTERIIAM
                     LEVSGVDGLCPP"
     gene            7157..>7705
                     /locus_tag="AC16QF_14590"
     CDS             7157..>7705
                     /locus_tag="AC16QF_14590"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR6449836.1"
                     /translation="MAAPLEDITVVAIESWMAAPSASALLADLGANVIKVEPPSGDPM
                     RNLSRAPKVDGPLKALDCQFDVDNRGKRSVAVDLDTDQGKAVVHRLVARADVFMCNLL
                     IHRQERFGLDPDSVLALNPRIVHATLTGYGTTGPDAWRPGYDVTAFFGRSGLYDASRE
                     GDDGDVPMARPAQGDHTTGLALL"
BASE COUNT         1412 a         2641 c         2463 g         1189 t
ORIGIN      
        1 ctcgtacgcc cagggcctgc ccaccaggga gaaaacggcg acgacgagcg caaagcccag
       61 ggcgacgctc atgatcggga aaagcacctg acgctcggca aacccgaacg agcgcagggc
      121 cgtcatctcc cagtcgctgt agagtcgccc gagggcaagc accagggcga cgaagaggct
      181 gaccggcagc agcacttcct gagcgatgaa agacttgagc gccgtaagct gcagcacctc
      241 cgacgtccgc agcaaaccgt cggcagcctt gtcgaggaac cgggtcatgc tgtaggtggt
      301 gaatatcacc gtcaacacgc cgccaaccgc gaaaaacggc agcagaagct cgcgaacgag
      361 gtaccggttg atgatcagtg ctctaaccct ctcccgaact gcaggccagc ccgtcgccgg
      421 atcctgatat catcgacccg gtttcccgac gaccccggcc gagctccatg ctgaaaagat
      481 ttttcgccgc ggaactggct tcggccattg cgctggcgtt ggtgcagctc tcttgctcgg
      541 catgggccgg cgagctgccc gcgctggacg atcaagatac cgtagttgtc accgccgacc
      601 aagcttggga gtccgaggac ggcgactcgt tgagctttgc cggcaacttc aacctcaacg
      661 cccccgactt ccacgtcgca tccgacacgg cggtcattca cgggcctttg gaggatccca
      721 accgcatcgt cgcggagggc agccccgtgc gcttctgggt tgccgatccc gattccggga
      781 acaggactca cggaaccggc aggcggctgg aatacgatcg gcaaagggat ctggtccggc
      841 tcgaggggga tgccgtactg gaagacgacc ggaccgtgat gcgaagttcc gagctcgaat
      901 acaacaccgc cacgaaacga ctcgtcgcgc aaggatcggg cggcgtggag atcgttacga
      961 acccgaaccg cgactgagca cccggctcag ctccaggcct cacgcaactg cccgagcagg
     1021 gcttcacgtt cggccgcgtc gagctcatga aactccccca gggccggcgg tccgggccag
     1081 ccgtggtcgg ctatctccac cgtccccagc agacgggcgg cctcgtagcg gggaatgacg
     1141 tgatagtgca cgtcggggtc gaccatcatc agcatcaggt agttgatcct gtcgtagctg
     1201 tacaggctcg acagggcccc ttcggccgcg gctatcacgt cgtgaagctc ggtgaacgcc
     1261 gccctgctga cctcggagaa tcgggtgaca ggctctttgc agaccaggac cagggagccg
     1321 agagagacct gctgcggccg gacgagaacc agccagtgac cgtactcgcg caccagcgtg
     1381 tccggatagc cgaatttctc tgccgtggcg ttcaagtcaa tccgccgccg cccgcgaggc
     1441 gggcttgcgc caggcagtga ccacgcccgc cgcgttgtcg agatcgtccc ggtcgtcgac
     1501 ttcgcaccat gacagaccgt gaatgtcgca aaccccaacc tcgccgctct gagcgagctg
     1561 atcaacggca gagaggtacc agagcttcaa acccatgtcc gaacgcatca gcgactccac
     1621 cttgctcacg aacttggcag gacccagacc cctgaatgcc atcatgccga tggactcacc
     1681 gttcacgtcg tccagctcga gtcgcttgcc cacacgccga agacgcccgt cgctgaccac
     1741 gactttcatg tcgtcctcgt cgtagtgatc cttacggtcc accacgagcg taacgggcgc
     1801 attcgcggga gatgccagca gcttctgcag gatggccgcc tcgaacagcg tgtcgccgtt
     1861 gatgatgacg aaggggtcgg tcatttcgct gcgcgcgacc cagcaggtgc caaggttgtc
     1921 gctcgcatcg aagaaggggt tgtgcagggt tctcaccggg accatgctgc agcggctgac
     1981 caggtcatcg acgatgtcgg catggaaacc ggtaacgacc acgacctcgt cgatgccgca
     2041 ttgagccacc tcgccgagct gccattccag caggctctgg ccggcgatct cgacgccgca
     2101 tttgggcgtc ccggccgtca gcggcagcag cctgcggccc tgaccagcgc tcagaatgat
     2161 ggccttcact agggtaccgg tatcgggaaa acgcgaaggg tcggggtttt caccgcgagg
     2221 gtcaagactt tccgcccagc aggccgtgca gatggtctcc cagccgcagc gccagggcca
     2281 gaatcgtcag ggtggggttc gcccagccgg cggtggcaaa gacggagctg ccggcgacga
     2341 acagattgcc gtagccgtga acgcgcccac tcccgtccac cacaccgtgc cgaggatcgg
     2401 cactcatgcg agtggtgccg atgtggtggt acccgccgat ggggtggttg cccacggtcg
     2461 ggtcaacggg ccagtcattg cctggctcgc ccagccagga actcgcctcg acgaaaccca
     2521 ggcccagccg ggtcagctcg ccgtccagca gccgcaccat cacctggatc gtgcgcttgt
     2581 ccagctcgcc gagctgccag ctcagggcag cccggggcat gcccagggcg tcacgctcgc
     2641 tgcccagggt gacgcgactg tccgggttcg gggcctgctc ggcccggatc atgagataaa
     2701 ggcggcgcat gccgaggccg gcccgcagcc gctcgaaggg aacccgggca agccgctggg
     2761 ccgagctgcg cacggccctg taggcgtgcc acagggtacg cccccgccgg gtcggcgcca
     2821 tgccgtgctt cagggtctga tacatctgcc gcgttaccgc gaccccgcgc tccggatccc
     2881 gctgcagact gagcgtgaac gcagaattga gtatcccctc gcgctcctga gcggccgcag
     2941 acgggacgag aacgggcgcc agatccggtc cgtgactgcg aaagcgcttg cggaaggccg
     3001 cccacaaggg gaacgcgccc ggccccgaga cccggcccgc gcggccgtgc gggtgctcca
     3061 tgaagtagcg cccgacctgg tcgttgccgt tgccgacccc ggccgcctcc acatcgcggg
     3121 aggccagcag gagacgggca ttttcgatcc cgccgcaggc gagcacgtag tgccgcgccg
     3181 tcacccgccc ctcaatcccg gaaagcgaag acactttcag atgctcgatg ctggccgcgt
     3241 tggccgccgc ctgaatgtgg gtcacgttgg cgtgcagaac cagagtcacg gcagcgctgc
     3301 tggccaggtc ctgcacccga cgggcggcga agcgttcccg cacgtcgtcg aagcgccaga
     3361 atcgcgtgct gaccgcgtcc gggtcgaagg gcggcacccg ttcgccgacc tcgtcccaca
     3421 acgattcgtc gtaacgcacc tcgccgagct cgagctcgtc gtgcacggcc gcgtagtaag
     3481 gcgtgagctc cgcattggtg atgggccagc cgctgtgcgg cacccacggc ctgcgccgga
     3541 aatcgatgtc gtcgagcaag gcgcagcgcc cgccccagat gttggtggtg ccccccagaa
     3601 agcgcaggcg ggcatcgacc aggtcatagt aggtcatgcc gtcgctggtc ccggcgtaga
     3661 gatcctgggt ggactgctcg aaatccaggc cgccgctctc cagcaggcag atggagtgac
     3721 cgagctccgc caggcgccgg gccagcgcca caccggctgc gccggcgccg gcaatgcaga
     3781 cgtcggcctg gaaacgctcg ccctggggca gcttttcgag atcgagcagc acgactaagc
     3841 ctcgcgcctc gcccggttcc agtcggccag gttcttcagc ggatccttca cgtaggcgct
     3901 gccgcggatg agcgtgacga tcagtcggtt gaaccacgtg cccaggcggt ccgacggccg
     3961 gtccacatcc agaaacagca cgatgcgtgt ctcgtccgtg tcgtttcgca cctcgtgctc
     4021 gaacgtatca tcgaaaagca gacagccgcc cgcctcccag tggcggatct ggtcgtcgac
     4081 gcgcagccag caccgttctc cgtcacgggg cacggcaaga cccaggtggc aacgaatcac
     4141 cgcacgggta ggccccttgt gcggcgggat gtggtagcgg ggcgcgagga tggagaacca
     4201 ggcgttctgc agacccggaa ggcattcgat ggtcgcagcc gtgaccgggc atcgccggca
     4261 gttttcttcc aggcggatac cgaacacgta gaaggcgaac gtcttccagt tgtcgccctt
     4321 ggagatgcgc ttctggtccg gcgacagctg atgaaacgcg gggatgtcct ccgggtgttc
     4381 catgaccgta gccagctcgg atcggatctc ctcccagcgc ccctccagcg agggtaccca
     4441 gggaaattcg gcattctcca gaaacggtcc cgtgcccacc agagagtggc ggccctggaa
     4501 ccggccgatg gcccgcagca gacgtttgcc gcccttgatc acccagcgcc tgggaagcga
     4561 agcggtcccg ctcacgacgc ctctccgacg atccgctgcg cgagccgcca gtcgtcgacg
     4621 ctgtccacat ccaccgcggc gcgcggctcg tccacgatca ccgatcccaa ccggcagccg
     4681 gcgcgctccg acaacgcccc gagcgcggcc cccagagtca gccgtcccgt caggtagcgc
     4741 agcagcaggc gccatccgag ccgacgcgcg atgcgccagg gacgcttgcg atccctctgc
     4801 acggccaccc agaactcgat gccccgccgc gcctcctcgg tgcgcagaaa atagaggttg
     4861 gcgccgcatc tggcaccgtc ggcgaacttg agcaccgttc tgcggctttc cggataggcg
     4921 gcccgcaccg cctggtaggg aaccaggccg acgatcaggt ccgcctgcac ccggtgcgca
     4981 gcctcgcaga aagcgtcgac gatggatgcg cagagcaacg cgtgatcgcc gctggtgacc
     5041 agcgtcggcg gtgcctggcc ttgcaggccg gcaagtgcgc tggaggccgg gtcgccggcc
     5101 ggagcaaccc aggccgcccc ggcagcgtcg atcattgcct taacctcagg atgggccgac
     5161 agcacctcgg gggacgggcc caccaccaca gccgggcgca cgagttccgc gcgcgaaagg
     5221 gcccccagaa cgcgctcgat ggccggcacg ccgcccaccg gaacgagaac ttctacgtcg
     5281 acgcccgcct gggcgcacaa aggcgtcggg ccggcacgct ggccggcgag caccaccgcg
     5341 ccgcaaacac tcacctcgct cacgacccgg ggcctcgaga aagcgacttc tcgaacatgg
     5401 cataggtccg ggtgacttcc cccccgaccg cctcgagaat gctgcgcatg ccctgattgt
     5461 cttccaggat ccaagaaagt tccgcccgct cgagaccggc acgatgggcg ggctcacgca
     5521 gggcgctgat gcagaacatg gccaggcccg gaccgagccg cgtgcggtga aagcgccggc
     5581 gaacgcccat gagcggcacc cggccggact tcggatagcg gaccttgatg cgccagagca
     5641 gcttggccca gccgaacgga agcagccggc cgtcgagatc accgatgact tcgttgaggt
     5701 tcggcagcat gacgatgaac gctgccggct cgccgtcgac ctcggctatc tgcacgagat
     5761 ccggcgggat cagcatcatc atttccttac ccacggcaag aaactctctg gacgtccagg
     5821 ggacgaactt ccagttctcg gaccaggcat cattgaagat gtcgcgcagt atttcgagct
     5881 cttcgacgag cttgcgccgg tcgagcgtgc gcattgtcac ccgcgggccc aactgcttca
     5941 tcatgatgct catcagccgc ggcctttcga agagcacggg catctcgtag gcaagcatgc
     6001 gctgcacctc ggcgtaacca cgccgcgcca gcgcttcgcc gtaatagggc attccatgcc
     6061 ccatcatgaa gaagggcgac gaatcgaagc cgtcaacgag caggcccacc tcctggttga
     6121 tgttcaggtt gaacggaccc accacgtgct cgcagccacg ctcggccagc cacgcctcgg
     6181 cgcttgcgag caggccgtcg atgacggcgt cgtccgggat gctttcgaac atgccgaaga
     6241 acccgccctt ggggtcgtgg cgttgctgat acagctcgtc gatctgggcg gagatgcggc
     6301 cgacggcccg gccgtcgcgg tacgcgacga acggctgcca gcgggcgtgc tcgaagaagg
     6361 gatgcttggg ggacaacgcg tcccggcgct ccatcttcag cggcggaacc cagcacgggt
     6421 catccgcata cacgacccag ggcacctcga tgaagtcgtt aagcccggac cgtccggaca
     6481 cgggtacgac gctcaatgac atgccgaagc gaacgttatg ggggacacaa gccgtcgacc
     6541 cccgacactt cgagcatggc gatgatcctt tcggtgcagt tgccgaccgg ctccatcact
     6601 tcggtgtgac cgaacatcag cacccaggcg cgaagcctaa ggttgccctc atcatccagc
     6661 cggatgtaac tggaataggt cctgcctgac cccgggtcgt agaaatcccc gcgccagcgg
     6721 cccctcttga aacgatagtc gctcaacagc tgcagcccga tcagcggccg gtcgcgcagc
     6781 tcggctttag cgtttttgcg gtcgggtcgg ggtttgcgca gagcgacgac ggtggcttgc
     6841 agggtgtccc cggagcgctc gatggcaaac acggcaccct tggaggccca gaccccctct
     6901 acgcggctgg ctttggcgtc cgtgtctacg gtttggttgg ccgaggcgac gggtgtccag
     6961 agcacgcccg tcacgagcat gaggatcgaa gcagccgccg cccacggccc tcgcagatag
     7021 tgcagttccc gcatggcgcg caaggttacc gcgccgccga agctactgcc agttctcgcg
     7081 cggaagctga atttgccgcg aatatgcggt acaaccgggc tttcacgccc cgtgagctca
     7141 aagaggaggc gagccgttgg ctgcaccgct ggaagatatc accgtcgtcg ccatcgagag
     7201 ctggatggcg gcgccgagcg cgagcgccct gctggcagac ctcggcgcaa acgtgatcaa
     7261 ggtcgagccg ccgagcggcg atcccatgcg caatctctcg cgcgcgccca aggtcgacgg
     7321 tccgctcaaa gcgctggact gtcagttcga cgtggacaac cgcggcaagc gatcggtggc
     7381 cgtggacctg gacaccgacc agggcaaggc cgtcgtgcat cgcctcgtcg cccgggccga
     7441 cgtgttcatg tgcaacctgc tcattcatcg gcaggaacgt ttcgggctcg atcccgacag
     7501 cgtcctggcg ctgaacccgc gcatcgtgca cgccacgctg accggctacg gcaccaccgg
     7561 gccagacgcc tggcgccccg gctacgacgt gaccgccttc ttcggccgct cgggtctcta
     7621 cgatgccagc cgcgagggcg acgacggaga cgtgcccatg gcgcgcccgg cccagggcga
     7681 ccacaccacc ggcctggcgc tgctc
//
LOCUS       DBNHLM010000319         4842 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1184754, whole genome shotgun sequence.
ACCESSION   DBNHLM010000319 DBNHLM010000000
VERSION     DBNHLM010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1184754"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(347..1621)
                     /locus_tag="AC16QF_14595"
     CDS             complement(347..1621)
                     /locus_tag="AC16QF_14595"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6449837.1"
                     /translation="MSDARSVADYHFLKLNDEVLQNPYPFFRRLREEGGVVREADYDT
                     FLVGSLDAIREVNRQPEVFSSVLVANAPYVPLPCPLDEIAQWRDANPHSDKILSNDPP
                     DHTRHRKLVNRFFTTSRVRDLEPRIRQLTNEIIDTFVDLGEVEFVFGFAHMLPRLVVG
                     ELIGLPEQDQPRFRRHFEDRLALMARAAEKPLEGFQQRTTQASLTEDTFLRDYFVDAI
                     NERREQPRDDIMSELARATFEDGAPVPLESLVSMISLLYGAGGDANTPELMTNAMLVL
                     LDRPDLERALRDDPSLSKAFIEEVLRYDTPVPGVFRIALQNTSIAGTPVPKGSKVMVV
                     YGAANHDEAYFDDPETFVMGRDCPHPHLGFGLGVHFCPGAPLARLEGQVAVQELLRRL
                     TNIRRADNEPIPYVPTVIQRIPIQVRLRFDKA"
     gene            complement(1618..2436)
                     /locus_tag="AC16QF_14600"
     CDS             complement(1618..2436)
                     /locus_tag="AC16QF_14600"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6449838.1"
                     /translation="MPITIANGIDLYFERRGDGAPLLFISGSHGDLRARPNQFDTPLA
                     RAFDMIAYDQRGLGQTGKPPGEYTMAQYADDAAALLDALRLDAVPVVGVSFGGMVAQE
                     FALRHPDRVDRLILACTSSGGAGGSSYPLHELQALPPRERAEAHLRISDLRHDDEWVA
                     ANPERWERLVELTMAARRPDRDEQGAAKQLRARKGHDTYDRLPRLDVPVLLAGGRRDG
                     IAPVANMEALHRQIAGSRFELFDGGHMFLIQDKTAYPFIVGWLQNDWSELRDEP"
     gene            complement(2538..2613)
                     /locus_tag="AC16QF_14605"
     tRNA            complement(2538..2613)
                     /locus_tag="AC16QF_14605"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2578..2580),aa:Ala,seq:ggc)
     gene            2773..4008
                     /locus_tag="AC16QF_14610"
     CDS             2773..4008
                     /locus_tag="AC16QF_14610"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR6449839.1"
                     /translation="MTKNEAGRPELKRALNLPMLTLYGLGTTIGAGIYALLGEIAGTA
                     GYGAPFSFLIASLLAAFTACSFAELAARFPRAAGAALYVQKGLGTERLSTVVGLLVVA
                     SGLVSSAALVNGFQGYLQEFVELDRTIAIPAICAALAAIAAYGIVQSVTVAAAITVVE
                     IGGLILVIAVGADNFAALPERWPLMLPGTATTSWVGVLMGVSLAFYAFIGFEDMVDLA
                     EEVKNVRLNMPRGILLTLGITTVLYFVLMISALLALTPEELAASSAPMARLFEAGTGK
                     TSMLISVIALFAIINGALIQLVMVSRVLYGLGSRRKLPQWFARVNPKTRTPLVATLTA
                     AGVLFVLALSGHLAALAKTTSLLMLTIFMLVNLALWRIKSTEAQAPDDLPTFPRIVPA
                     MGALLSGLFVLRELSTRLF"
     gene            complement(4048..>4842)
                     /locus_tag="AC16QF_14615"
     CDS             complement(4048..>4842)
                     /locus_tag="AC16QF_14615"
                     /inference="COORDINATES: protein motif:HMM:NF039902.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HIR6449840.1"
                     /translation="WEMPVLCHLPLLRNPDQSKLSKRKNPTSVNYYRRIGILPEALVN
                     YLGMMGWSMPDERELFGPQDMADDFELGGIHLGGPVFDLEKLSWLNGQYLRALSPDDF
                     MQRISDWMLNKQALSALVPLVQERTERLSDLVGQVDYLLGDRRSVEATDFEHKTLSRE
                     QVVLVLDHALREFDAMRRWERDDLFARCKALADGLSLKIREFLFPLFVAVSGREVSLP
                     LFDSLVFLGPDVTRMRIRDALAVLGVSRKESKRLEKRYQEYLSTRP"
BASE COUNT          901 a         1544 c         1466 g          931 t
ORIGIN      
        1 gtgcatcgaa gagtcgcacc tccgaggctg tttccgtcaa cttgctccga gagcacgatc
       61 tgcgcgaaca accgttcgga gctctgctgc ctggcggacc ctctccggtt ggtgaagctc
      121 gctctagccg acggatcggc ataggactcc acggctttta ttttcatggc gtctttttat
      181 tctgccaatg ctatttcgcg gccatcctag cctgacaact cacccttcct gaatgagcgc
      241 aatcccccgc cagcagaaac gttccgctga cttgttttga atcatacacc ccagaacgcg
      301 ttttgaaacc atcaggaaaa ctacctaatt atccgtaatg gcgcggtcaa gccttgtcga
      361 aacgaagtct cacctgaatg ggtatccgct gaatcacggt gggcacatag gggatcggct
      421 cattgtctgc tcgtcgtatg ttggtcagcc gacgcagcaa ttcctgaact gccacctgac
      481 cttccagccg agcgagcggc gcacccggac agaagtgtac tccgagacca aagcctaggt
      541 gcgggtgcgg acagtcgcgg cccatcacga acgtctcggg atcatcgaaa taggcttcgt
      601 catgattggc ggcgccgtag accaccatca ccttggagcc ctttggcacg ggagtgcccg
      661 caatggacgt gttctgcagc gcaattcgga acaccccggg gaccggggtg tcgtaacgca
      721 acacctcctc gatgaaagcc ttgctcagcg agggatcgtc acgcagcgca cgctctaggt
      781 cgggccgatc cagcagcacc agcatggcgt tggtcatgag ctcgggcgtg ttggcatcac
      841 cccccgcgcc gtaaagcagc gagatcatgc tgaccagcga ttccagagga accggcgctc
      901 catcctcgaa cgtggcccgg gcaagctcgc tcatgatgtc gtcacgcggc tgctcccgtc
      961 gctcattgat ggcatcgaca aaatagtcgc gcagaaaagt gtcttcggtc aggcttgcct
     1021 gggtggtgcg ctgctgaaag ccctccaggg gtttttccgc ggccctggcc atcaacgcga
     1081 gccggtcctc gaagtgacgg cgaaaccgcg gctgatcctg ctcgggcaag ccaatcagct
     1141 cgccgaccac cagccgtggc agcatgtgcg cgaacccgaa cacgaactcc acttcgccga
     1201 gatcgacgaa ggtgtcgatg atctcgttcg tcagttggcg gatgcgcggc tcgaggtcgc
     1261 ggacgcggct ggtcgtgaag aatcggttca ccagctttcg gtgacgggtg tggtcaggcg
     1321 gatcgttgga gagaatcttg tcgctgtgcg ggttggcatc ccgccactgg gcgatctcat
     1381 cgagcgggca aggcagcggc acgtagggcg cgttcgctac caggacgctc gagaacactt
     1441 ccggctggcg gttgacttca cggatggcgt caagagaacc aacgagaaac gtgtcgtaat
     1501 ccgcctcgcg gactacaccc ccctcctcgc gcagccggcg aaagaaagga tagggattct
     1561 gcagaacctc atcattgagc ttgagaaagt ggtagtcggc tacagatctt gcatcgctca
     1621 cggctcgtcc cttaactcac tccagtcgtt ttggagccat cccacaataa acggataggc
     1681 ggtcttgtcc tgaatcagga acatgtggcc gccatcgaaa agctcgaacc ggcttccggc
     1741 aatctgccgg tgcagggctt ccatgttggc aacgggcgcg atgccgtccc tcctgccgcc
     1801 ggccagaaga acgggaacgt cgagtcgggg cagacgatcg tacgtgtcat gccccttgcg
     1861 ggcccggagc tgcttggccg cgccctgctc gtctctgtcc gggcgtcgcg cagccatcgt
     1921 cagctccacc agcctctccc agcgttccgg gttcgctgca acccactcgt cgtcgtgacg
     1981 cagatcggag atccggagat gggcttcggc tctctcccgg ggaggcagcg cctgaagctc
     2041 atggagcggg taagaggatc ccccagcgcc tcccgacgac gtgcacgcga gaatcaggcg
     2101 gtcgacacgg tccggatgac gaagcgcgaa ctcctgcgcg accatgccgc cgaacgaaac
     2161 gccgaccacg ggaaccgcat cgagccgcag cgcgtccagc aatgcggccg cgtcgtcggc
     2221 atattgcgcc atggtgtact ctcccggcgg cttgccggtc tggccgagcc ctctctggtc
     2281 gtaggcgatc atgtcgaagg cccgggccaa cggggtatcg aactgattcg gccgggcgcg
     2341 caggtcgccg tggctcccgc tgatgaacag cagcggcgcg ccatcgcccc gacgctcgaa
     2401 gtagaggtcg atcccgttgg ctatggtgat tggcatgctg gccctcggcg ctgacacgtt
     2461 cgtttatatg ccaagcgccg catcaaggcc accggtcagg aaaggcgggg tctccaacct
     2521 caagtcattg atttaattgg tggagccaga cgggatcgaa ccgtcgacct ctacaatgcc
     2581 attgtagcgc tctcccagct gagctatggc cccacgatgc cgtctcggcc gagacgggag
     2641 gagggagcgg atggtaagaa cccggcgatc cggagtcaac atgtcaccct ggccgagccg
     2701 gtcgcagaat ccctagatgc atcccatcga ttccccgcct atggttgggt cctgctccag
     2761 gaagcaggga acatgaccaa gaacgaggca ggccgaccag aactcaaacg tgcgttgaac
     2821 ctgccaatgc tcacgctcta cgggttgggc acgaccatcg gcgcaggaat ctacgctctg
     2881 cttggcgaga ttgctggcac cgccggttac ggggcgccct tctccttcct gatcgcatcg
     2941 cttttggcgg ccttcaccgc gtgttcgttt gcggagctgg ccgcgcgatt cccgcgtgcc
     3001 gccggcgcgg cgctttacgt gcagaagggc ctgggaaccg aacgcctttc caccgtggtt
     3061 gggctgctcg tcgtcgcctc gggactggtt tcctctgccg cgctggtgaa cggctttcaa
     3121 ggctatctgc aggaattcgt agagctcgac cggaccatcg ccatcccggc aatctgcgcc
     3181 gcgcttgccg ccattgcggc ctacggaatc gtgcagtcgg tgaccgtcgc agcggcgata
     3241 accgtcgtgg agatcggcgg cctgattctg gtcatcgccg tgggcgccga caacttcgct
     3301 gcgctgcccg agcgctggcc actgatgttg ccgggcacgg ccaccacttc gtgggtaggc
     3361 gtcctcatgg gcgtcagcct cgcgttctac gcgttcatcg gcttcgagga catggtcgat
     3421 ctcgccgagg aggtcaagaa cgtcaggctc aacatgccgc gcggcatatt attgaccctg
     3481 ggcatcacca ccgtgctcta cttcgtgctc atgatctcgg ccctcctcgc cctgacgccc
     3541 gaggagctgg ccgccagcag cgcccccatg gccaggctct tcgaggccgg caccggaaaa
     3601 acctccatgc tcatcagcgt catcgcgctg ttcgccatca tcaacggcgc cctgatccag
     3661 ctcgtgatgg tctcccgcgt tctgtacggg ctgggttctc gccgcaagct gcctcagtgg
     3721 ttcgcacgcg tgaacccgaa gacgcgcaca cccctggtgg caaccctcac cgctgccggc
     3781 gtgcttttcg tgctcgctct gtccggccat ctggcagccc tcgccaagac aacgtcgctg
     3841 ctcatgctga cgatcttcat gctggtgaat ctggcgttgt ggcgtatcaa aagcacagag
     3901 gcgcaggcgc ccgacgacct gcccacgttt ccgcgcatcg tcccggcaat gggtgcgctg
     3961 ctcagcggct tgttcgtgtt gcgggagctg tctacccggc tcttctgact gccggtgaga
     4021 tcatcacttg cagatcacga cggccggtca tgggcgcgtc gagagatact cctggtagcg
     4081 cttctccagc cgtttcgact ctttcctgga gacgccgaga acggcaagcg cgtcgcggat
     4141 ccgcatgcgg gtgacatccg ggcccagaaa caccagggaa tcgaacaacg gcagcgacac
     4201 ctcacgaccg gaaacagcca cgaagagcgg gaagaggaac tcgcgtatct tcagactcag
     4261 accatcggcg agcgccttgc accttgcaaa caggtcatcg cgctcccagc ggcgcatggc
     4321 gtcgaactcg cgaagggcgt gatcgagaac cagcacgacc tgctcgcgag aaagcgtctt
     4381 gtgctcgaag tccgtcgctt cgacgcttcg gcggtcgcct agcaggtagt cgacctgccc
     4441 gaccaggtcg gagagccgtt ccgtgcgctc ctgcacgaga ggaaccagcg cagaaagcgc
     4501 ttgcttgttc agcatccaat cggagatccg ctgcatgaag tcgtccgggc tgagcgcccg
     4561 caggtactga ccgttcagcc aggagagctt ttccaggtcg aagacggggc cgcccagatg
     4621 aatgccgccg agctcgaagt cgtcggccat gtcctggggt ccaaagagtt cccgttcgtc
     4681 aggcatggac catcccatca tgccgaggta gttcaccagg gcctcaggga ggattccgat
     4741 ccgccggtag taattgacgc tggtggggtt cttgcgtttc gacagcttgc tctggtccgg
     4801 attccgcagc agcggcagat gacacagaac gggcatttcc ca
//
LOCUS       DBNHLM010000320         5035 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_228352, whole genome shotgun sequence.
ACCESSION   DBNHLM010000320 DBNHLM010000000
VERSION     DBNHLM010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5035)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_228352"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="AC16QF_14620"
     CDS             <1..612
                     /locus_tag="AC16QF_14620"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF003743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HIR6449841.1"
                     /translation="IFILGDLCEAWVGDDDDSPLATALRAALKSAGEQTSLHVMRGNR
                     DFLLGERFAAEIGCCLVEDPCLIDPDTLIAHGDAFCIDDEAYQAMRRVLRSEAWQQDV
                     LARPLEERRGLAAQMREQSRLSNANKAENIMDVSADEVDRTVKEYGARRLIHGHTHRP
                     GVHPGDWGTRYVLGSWEHCGWLLREQDTVTALECFPLSGRYGT"
     gene            complement(566..1204)
                     /locus_tag="AC16QF_14625"
     CDS             complement(566..1204)
                     /locus_tag="AC16QF_14625"
                     /EC_number="1.14.99.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 - tRNA
                     2-(methylsulfanyl)-N(6)-isopentenyladenosine(37)
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaE family protein"
                     /protein_id="HIR6449842.1"
                     /translation="MSATRSRAKPEDAAEFLKCSTPVLWFEQAAGNLETLLVDHANCE
                     KKAAGTALSLIYRYVDRPELLQRLSRLAREELRHFEQVHALMCSMGIAYHALAPGRYA
                     ASLMQQVRTHEPARLVDTLLIGAIVEARSCERFAGLVDVLPGGVGELYAALMVSEARH
                     FRGYLDLAQRYGDGSLEERLDHLLGFESELASGPDTQFRFHSGPIAGNIPAQ"
     gene            complement(1215..1661)
                     /locus_tag="AC16QF_14630"
     CDS             complement(1215..1661)
                     /locus_tag="AC16QF_14630"
                     /inference="COORDINATES: protein motif:HMM:NF018630.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HIR6449843.1"
                     /translation="MTATRRSTPCVGICSTTYGDLVCRGCKRFAHEIVSWNAFDVDQR
                     EAVWSRLEELRTGAIARCLRVLDEERLIAEAEAVRVPQAAQRELLDLAYEALRRWRDR
                     DAPPERLGVEALAGADTAQDLIRQIDREFYSRSLAHYERSFKIAAQ"
     gene            complement(1672..3693)
                     /locus_tag="AC16QF_14635"
     CDS             complement(1672..3693)
                     /locus_tag="AC16QF_14635"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF023418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase TgpA family protein"
                     /protein_id="HIR6449844.1"
                     /translation="MASARASSGVGFAYQIPRNSLALLMFAQVVVVLPFALQLSPWII
                     AVGLFCGYWRAGVYQGRWSYPHRWVKALLVLASVVGVAVSGAGAFSLEAAASLLILAF
                     ALKLIEMKSRRDAYLVIFLAYFVIATQFLFDQSILVAVYELGAICVVTAAMIGLNQLH
                     TRVRPLASLRDASKLVLQALPFTLVLFLLFPRIAPLWTVPLPTAGKTGITDVVKPGAI
                     AALTQSDELAFRVVFEGEIPRYRDLYWRGLVYSEFEDGAWSVGPELPAVPSSLRPEPG
                     RGEVAYEVLLEPTMSDWLFALDTAFPRSAGVASTQDYRLRARDPILSVFRYGVASYPD
                     FVMDERLTGRLITRETRIDLQDSPRAVALARSLHDGDDERFVDAVLELIRQEPFRYTL
                     NPPRLPDAGSIDAFWFESRAGFCTHYAGAFVYLMRSVGIPARMVGGYQGGEINPISNH
                     LMVRQYDAHAWAEVWLRGKGWQRVDPTAAVAPERIERGLSAALSANDRARLSALTSAR
                     LGAWSTLADMLYWVDSLEHRWNLWVIGYDSRFQAGFLERLLGKVTPGRTGLVMLAGGV
                     ASIGLVAVFLFWRRRPRKRHPVERMFLRFAGKVSGYGLQRNPQESPGAFVRRAARNAG
                     WRDEQADQIVAQLNALLYNPAVSWGRAEVASLAAQLRRLQFRLAFASAR"
     gene            complement(3674..4642)
                     /locus_tag="AC16QF_14640"
     CDS             complement(3674..4642)
                     /locus_tag="AC16QF_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6449845.1"
                     /translation="MADTAAHAFVGQRFGRWLDRRIPPGSRITLSQRNVFIFPTPTGF
                     VFGGLLILLVLGAINYQASLVYGVAFLLGSVFLMTILYTFRNLSGLSLELVSTRAGFV
                     GEAVEFSVRVVRPKGAGREGIQLGWPNGIKQWAELYEQEADTLRLYVKGERRGYLNPG
                     RLLVETYYPLGLLRAWTWVDLDARALVYPKPIFQDFPLSEQGRRDEGELIDPLGSEDF
                     TDIKGYQPGDPIKHIIWRSYARSNDVMVKRYASYLEPRLWLDLETVAGPLEERLSRLT
                     GMALTATRLEREFGLRLGGLEIKPATGETHLEQVLRELALHGIRES"
     gene            complement(4648..>5035)
                     /locus_tag="AC16QF_14645"
     CDS             complement(4648..>5035)
                     /locus_tag="AC16QF_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6449846.1"
                     /translation="ELLRGGDRRAVLEGLEPVSDLEGLRALQRAVDEVKVSDLLIEYV
                     QRLVAHSRRGSEFAFGLSPRGALALLHGARAWALINDRSHVVPEDVQAVLPSVVEHRL
                     RQAADFSGHGGGALAQKLLSSVDVVG"
BASE COUNT          949 a         1693 c         1628 g          765 t
ORIGIN      
        1 attttcattc ttggcgacct gtgcgaagcg tgggtaggtg acgacgacga ctcaccgttg
       61 gccaccgcgc ttcgcgcagc gctgaagtcc gccggcgagc agaccagtct gcacgtcatg
      121 cgaggcaaca gggacttcct gctcggcgag cggttcgcag cggaaatcgg atgctgcctg
      181 gtcgaggatc cctgtctgat cgaccccgac accctgatcg cccatgggga cgccttctgc
      241 atcgatgacg aggcctatca ggccatgcgc cgcgttctgc gctccgaggc gtggcagcag
      301 gacgtgctgg cacgcccgct ggaggagcgg cgcgggcttg ctgcccagat gcgcgagcag
      361 agccgcctga gcaacgccaa caaggcagag aacatcatgg acgtctcggc tgacgaggtg
      421 gatcggacgg tcaaggagta cggggccagg cggctaatac atggtcatac ccatcgcccc
      481 ggcgttcacc cgggggactg gggaacccgt tacgtgctcg gctcctggga gcattgcggg
      541 tggctgctgc gcgagcagga caccgtcact gcgctggaat gtttcccgct atcgggccgc
      601 tatggaacct gaactgagtg tccggacctg acgcgagctc ggactcgaag cccaacagat
      661 gatcgagcct ctcctccagc gaaccgtcgc cgtacctctg cgctagatcc aggtatcccc
      721 tgaaatggcg cgcttccgag accatcagag ccgcgtacag ctcgccgacc ccgccaggaa
      781 gcacgtccac gagcccggca aaccgctcac aggatcgggc ctccacgatg gcgccaatca
      841 ggagggtgtc aacgagccgg gccggctcgt gggtcctgac ctgctgcata aggctggccg
      901 cgtagcggcc gggagccagc gcgtgatagg cgatgcccat ggagcacatc agcgcgtgca
      961 cctgctcgaa gtgacgaagc tcctcccggg cgagacggga cagccgctgc agcagctcgg
     1021 gccgatccac gtagcgatag atgagactga gcgcggtgcc cgccgccttc ttctcgcaat
     1081 tggcgtggtc gacgagcaga gtctcgaggt tcccggccgc ctgctcgaac cacagcaccg
     1141 gtgtcgagca cttgagaaac tcggcggcat cctcgggttt cgcgcggctt cgggtggcgc
     1201 tcacagcgtg gcgctcattg agcggcgatc ttgaagctgc gctcgtagtg ggcgagcgac
     1261 cggctgtaga actcacggtc gatctggcga atcagatcct gcgccgtgtc tgccccggca
     1321 agggcctcca cgcccagccg ctccgggggc gcgtcgcgat cgcgccacct cctgagcgct
     1381 tcataggcca ggtccagcag ctctctctgc gcggcctggg ggacccgcac ggcttcggct
     1441 tcggcgatca gccgctcctc gtcgaggacg cgcaggcatc gggcgatggc gccggttcgc
     1501 agctcctcga gacggctcca gacggcctca cgctggtcca catcgaaggc gttccagctg
     1561 acgatctcgt gggcgaaccg cttgcacccg cggcagacca ggtcgccgta ggtcgtcgag
     1621 catatgccga cacagggggt gctgcggcgc gtggccgtca tgacgatgtc gctacctcgc
     1681 agatgcaaag gccaatctga actgaagccg ccggagctgc gcggcaagcg acgcgacctc
     1741 tgcgcggccc caggagacgg ccggattgta cagaagcgca ttcaattggg cgacgatctg
     1801 atccgcctgc tcgtcccgcc atccggcatt gcgggctgcc cggcggacga aagcacccgg
     1861 cgactcttgg ggatttcgct gcaggccgta gccggaaact tttccggcaa agcgcaggaa
     1921 catgcgctcg accgggtgcc gtttgcgggg gcgcctgcgc cagaagagga agacggcgac
     1981 gaggccgatg cttgcgacac cgccggcaag catcaccaga cccgtgcggc ctggcgtgac
     2041 cttgccgagc aggcgctcga gaaatccggc ctggaagcgg gaatcgtagc caatcaccca
     2101 gaggttccag cgatgctcca gcgaatctac ccagtacagc atgtccgcca gggtgctcca
     2161 ggcgccgagc cttgcgctcg tcagcgccga cagcctcgcg cgatcgttgg ccgatagcgc
     2221 agccgagagc ccccgctcga tgcgctccgg ggccacggcc gccgtgggat cgacgcgctg
     2281 ccagcctttg ccgcgcaacc aaacttccgc ccaggcatga gcgtcgtact gcctcaccat
     2341 gaggtgattg ctgatcgggt tgatctcacc gccctgatag ccacccacca tgcgcgccgg
     2401 gatcccaacg gaccgcatga gatagacgaa cgcccccgcg tagtgcgtgc agaagccggc
     2461 gcgagactcg aaccagaacg catcgatact gcccgcgtca ggcagcctgg gagggttcag
     2521 cgtgtagcga aagggctcct ggcgaatcag ttcgagcacg gcgtcgacaa agcgctcatc
     2581 gtcgccgtcg tgcagcgatc tggccaacgc caccgctctg gggctgtcct ggagatcgat
     2641 cctcgtttca cgcgtgatca ggcggccggt cagccgctcg tccatcacga agtccggata
     2701 ggacgccacg ccgtatcgga agacggacag gatcgggtcg cgcgcccgca gccgataatc
     2761 ctgggtcgac gcaacgccgg ccgaacgcgg aaatgcggtg tccagggcga aaagccagtc
     2821 gctcatggtc ggctcgagca gaacctcgta ggcgacttcg cccctcccgg gctcgggcct
     2881 gagcgagctc ggcactgcgg gcagctcggg cccgacggac caggcgccgt cctcgaattc
     2941 cgagtagacg agcccgcgcc agtacaggtc gcgataacgc gggatctcgc cttcgaacac
     3001 gaccctgaac gccagctcgt ccgactgggt cagagcggcg atcgcgccgg gcttgaccac
     3061 gtccgtgatg cccgtcttcc ccgcggtagg tagaggcacc gtccacagcg gcgcgatgcg
     3121 cgggaaaagg agaaacagca ccagggtgaa aggcagcgcc tggagcacca gcttggaggc
     3181 gtcgcgcaac gaagccagcg ggcgcacgcg cgtgtgcagc tggttcaggc cgatcatggc
     3241 ggccgtgacc acgcagatgg cccccagctc gtagacggcc accaggatgg actgatcgaa
     3301 gagaaactgc gtggcgatca cgaagtaagc gagaaagatc accagatagg cgtcgcggcg
     3361 gctcttcatc tcgatgagct tgagagcgaa cgccaggatg agcagcgaag ccgccgcctc
     3421 caggctgaac gcccccgcac cgctcacggc cacgccaacc acggacgcca gcaccagcaa
     3481 ggctttcacc catcgatgcg gatagctcca ccggccctgg tagacgcccg cgcgccagta
     3541 gccgcagaac aggccaacgg caatgatcca gggggagagc tgcagcgcaa agggcaatac
     3601 caccacgacc tgcgcgaaca tcagcagcgc caggctgttg cggggaatct ggtaggcgaa
     3661 gcctacgccg gaactagctc tcgcggatgc catggagggc cagctccctg agcacctgct
     3721 cgagatgcgt ctcacctgtg gcgggcttga tttccaaccc ccccagcctc agaccgaact
     3781 cccgctccag gcgggtggcc gtcagtgcca tgcccgtgag acggctcaaa cgctcttcca
     3841 gcggcccggc gacggtctcc agatccagcc agagcctggg ctcgagatag ctggcatacc
     3901 gcttcaccat gacgtcgttc gatcgcgcgt acgagcgcca gatgatgtgc tttatcggat
     3961 ctccgggctg gtagcccttt atgtccgtga agtcctcgct gcccagggga tcgatgagct
     4021 cgccttcgtc ccggcgcccc tgctccgaaa gagggaagtc ctggaagatg ggcttgggat
     4081 aaacgagggc ccgggcgtcg aggtctaccc acgtccacgc ccgaagcaat ccgagggggt
     4141 agtaggtctc gacgagcagc cgcccggggt tgagatatcc tcgtcgctca cccttcacgt
     4201 acaaccgcaa ggtatcggcc tcctgctcat agagctcggc ccactgcttg atgccgttcg
     4261 gccatcccag ctgaatcccc tcgcgcccgg cgccttttgg acgcaccacg cgaacgctga
     4321 actccaccgc ctcgccgacg aacccggcgc gcgtgctgac cagctccagg ctgagccccg
     4381 acagattgcg gaacgtgtag agaatggtca tcaggaagac gctgccgagc aggaaggcga
     4441 cgccgtagac caggctcgcc tgatagttga tggcgccgag gacaagcagg atgagcagac
     4501 cgccgaaaac gaaccccgtc ggggtcggaa agatgaaaac gtttcgctga ctgagcgtga
     4561 tacgcgaacc aggcgggatg cgtctgtcga gccagcgccc gaaccgttgc ccaacgaagg
     4621 cgtgagcggc tgtgtcggcc acggatgcta gcctacgacg tcaacgctcg acagcagctt
     4681 ctgtgcgagc gcgccgcccc cgtgacccga gaagtcggca gcttgccgca gccggtgctc
     4741 caccacgctt ggcagcacgg cctgcacgtc ttccggaacc acatggctgc gatcgttgat
     4801 gagcgcccag gcccgggcgc cgtgcagcag ggccagggcg ccgcgaggcg aaagcccgaa
     4861 ggcgaactcc gatccacgac ggctgtgcgc caccagccgc tgcacgtact cgatcagcag
     4921 atccgagacc ttcacctcgt ccaccgctcg ctgcagggcg cgaagccctt ccaggtccga
     4981 aaccggttcg agcccctcga gcacggcgcg ccggtctccc cctcgcagaa gctcc
//
LOCUS       DBNHLM010000321        16854 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_765768, whole genome shotgun sequence.
ACCESSION   DBNHLM010000321 DBNHLM010000000
VERSION     DBNHLM010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 16854)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16854)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16854
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_765768"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="AC16QF_14650"
     CDS             complement(<1..287)
                     /locus_tag="AC16QF_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449847.1"
                     /translation="MAQPELVEHLLRPEAYPHPCRAVRLVETHISWVFLTGDYAYKVK
                     KPVLFSFVDFSTARLRKHYCEEELRCNRAFSPELYLDVVEIVRDVEGRIRVG"
     gene            420..2465
                     /locus_tag="AC16QF_14655"
     CDS             420..2465
                     /locus_tag="AC16QF_14655"
                     /inference="COORDINATES: protein motif:HMM:NF014312.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HIR6449848.1"
                     /translation="MTPAKPIKATLIGLFAVCPLISPMVAHPNTAEQEKTGKELYEQQ
                     CARCHQRDGGGVDGLYPSLRNAPSLWLDRKTAIRGVLAGRADDTVVNSPLMPTHGYLG
                     NETVASTLTFLLQEWGPGGQPYTAEEVAAERLELLASQPAPHPGLPDVSPLADMDAVQ
                     YVTSSGPPMSVSEFDRARSLYYGHCTGCHGVLREGTAGSPLTPVLMRQRGTEYLQAVI
                     NYGSSAGMFNWGTSAALTAEEINMLARFLQHPVPQAPDMTEADVRASWSLEVPLKDRP
                     GVPQGRHPLDELFVVTLHDVAQIALIHGPSRRVIGTVATGQSPHRVRGSASGRYLYVI
                     ARDGSLTMIDLYARKPRRVASVRVGYEARAVGASRHPDYADRYVLAGAYWPPQLVLLD
                     GRTLEPLKLISTRGRTADGGRYHPEPRVSDIAGSYLRPEFIANVKETGRVYLFPYYAT
                     DRLQLVDIGTTAELRAGTFSQDKRYFITPADVNAISVVDTKEQRQVAQIPARVFAGGP
                     GVTYDDPEYGPVWSTSTMVEDEIISIGTDPEGHPNYAWEKVRSLKGPGSGSMFMASHP
                     ASTNLWVDSPLNADETVSQSVAVFDKRDLDAGYRSIDVAAIADVGEGPSRVLQPTYDP
                     TGREVWVVVWNPQDLNSAIVVIDDRTLKPLAVIDDPRLITPTRIYSLAALRDAPSEN"
     gene            2468..2884
                     /locus_tag="AC16QF_14660"
     CDS             2468..2884
                     /locus_tag="AC16QF_14660"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6449849.1"
                     /translation="MLNTLKLILALAATCYFGLAAADDIAYDGGELYQANCANCHGMY
                     GEGDGIVTPSLSVVLQDLRYLAERNDGEFPRWFVTEIIDGRELRLAHGPDGMPVWGEA
                     FTRMEGYGEQAQSRVEQKIDALVNFIESIQIAPEDE"
     gene            complement(2964..3848)
                     /locus_tag="AC16QF_14665"
     CDS             complement(2964..3848)
                     /locus_tag="AC16QF_14665"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6449850.1"
                     /translation="MKIRSAMVVMAKPKHKQFALERAKQLQQSCGARLRLVSFCWNAM
                     CEQSDVFEAHQRRAIKATIMRERESWLIDQVRDAGMAAADVSIEVQWTDNIAGWVSEK
                     AAANEFDLVLKSVNRSRTLVHTPLDWQLLEQCSKPVYISAPPRRSASGNVLAALDFRH
                     RDRKHQALNIRVLEAAQEFADMSGGKLHCVNVVEFSQVLSDLDLIDPRSVKKKALANN
                     QELMNALLEPFDVPKSRMHTPLGKVGQMIAITARKIDADLLVIGTSTRRKGGTVVIGN
                     SAQRTLEKSPCDLLAVHP"
     gene            complement(3876..4775)
                     /locus_tag="AC16QF_14670"
     CDS             complement(3876..4775)
                     /locus_tag="AC16QF_14670"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6449851.1"
                     /translation="MDSINNILVALSDTAEARFVLEKVLILAKAGQAKVHVVSVVYEG
                     IAELTIDEIEDSARLKTLILESAESALEELVDPLRAQLPAIECATLWNRNAWEGVLHA
                     AEAVEADLIVKPATLHEKFGDVVRTPDDWNLLRHASVPVMLVKPQAWVDEPVLLCAVD
                     PFDDAHANLNAAMLREGRALADMLGGELHVVCAYPLFEPWVGELGAVRSYAEIKENVE
                     REIIRRVDAIAARAGVKFAMLHAEEGYPTHTIGAVAELTGAELVIVGTHAREGVRGLL
                     LGNTSERILHAVATDVLTMHGTD"
     gene            4916..5818
                     /locus_tag="AC16QF_14675"
     CDS             4916..5818
                     /locus_tag="AC16QF_14675"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6449852.1"
                     /translation="MKQINNILVAGDSLNGMEAALAKAAKIEHYTGANVWVAVVIYDA
                     IAEEDPDRLPPEQQSQLVEALKAAERNGLRQLTEPYRSRIATLDDRVVWNKNAAEGLL
                     RVQTEVHADLIIKPISEHGFITDHLHTPLDWALMRGSQGAVLISKKSDWANPQRVLAA
                     IDVADTRHADLSREILLTAATMARTLDAELHVVTAFPSLGQTVNDLQVAMDYEGIKRD
                     MRENRTAAIARWIEELDIGISDSHVLEGKPELVIPQLASDLSATLTVLGTAARQGIRK
                     LLIGNTAENIIARLDCDILTVREP"
     gene            complement(5835..6614)
                     /locus_tag="AC16QF_14680"
     CDS             complement(5835..6614)
                     /locus_tag="AC16QF_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015275390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HIR6449853.1"
                     /translation="MEIFSTVIAVIIVGLLISAFRVLREYERGIVFMLGRFYKVKGPG
                     LIIIVPVIQQMVRVDLRTIVLDIPSQDLITRDNVSVKVNAVLYFRVVDPERSIINVED
                     YMEATGQLAQTTLRSVLGQHELDELLAERERLNSDVQAILDKQTDQWGIKVANVEIKH
                     VDIDESMIRAIAKQAEAERERRAKVIHAEGEAQAASKLVEAAQLLASQGEAMQLRYLQ
                     TLTEIAGEKSSTIVFPLPMNVMQSIERILEHSGVPKSGQSD"
     gene            complement(6618..7982)
                     /locus_tag="AC16QF_14685"
     CDS             complement(6618..7982)
                     /locus_tag="AC16QF_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR6449854.1"
                     /translation="MKAWLPILIACAGLALGFGEARPATGAQPAADGVVWLVDLDGPL
                     GPATADLIIRSLDDAQEAGARALVIRMDTPGGLDKAMRGLIKEMLSADLPVVTFVGPD
                     GARAASAGTYIAYASHIAAMAPATNIGSSTPVSIAPTPQMPDPRDAGETEGDAGRAPS
                     GDAMTRKVVNDAVAYLQGLAELRGRNIDWAEETVRLGANLRASEALEANVVDLITRDV
                     SSLLADIDGWVVELPNRTVTLDVANARVHTVETDWQHELLEVITDPTIAYGLLIFGVY
                     GLILEFYNPGMVFPSVIGIICLLLGAYGLQMLPVNYAGLALIVVGIGMMIAEVFTPTM
                     GVLGLAGVVAFVAGSMILMDTEAPGYGIPITVVAAFAAASAGLTIFAIGAAMKARRAK
                     IVTGVEAMIGASGEALEDFQGTGHVRAFGEIWEARCSEPVAAHAKVRVTEVDGLILVV
                     QPED"
     gene            complement(8092..8559)
                     /locus_tag="AC16QF_14690"
     CDS             complement(8092..8559)
                     /locus_tag="AC16QF_14690"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6449855.1"
                     /translation="MAISYRNVLIAIDASDEADEVLEAATTLDPRGAARFQVCTVIPP
                     VMGGVSGMDGASFAATWPLREMEETITKDISSHVRERVARFGITPDRVAVLYGRPAVE
                     IRAYAAKIGADLIVIGSHGRHGVPGVLLGSTANAVLHGAECDVLTVRVRPAEG"
     gene            complement(8572..9012)
                     /locus_tag="AC16QF_14695"
     CDS             complement(8572..9012)
                     /locus_tag="AC16QF_14695"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6449856.1"
                     /translation="MSYKHVLAAVDMTDEARQVLDQAREVASEHEAKLSIVTVVKPLT
                     HVYGGLDMVSFTQASVDFEEEARSLALSKAREMAADAGLEAADVHVMVGSPSGQIVAA
                     AEAHGADLIVVGSHGKHGLGLLLGSTANGVLHHAETDVLTVRIT"
     gene            9231..9680
                     /locus_tag="AC16QF_14700"
     CDS             9231..9680
                     /locus_tag="AC16QF_14700"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6449857.1"
                     /translation="MYKNILVAIDLMEGPAHQVLSGALAFGEETAQYTVVHVVEPQYV
                     QYSFDPTFTGSLTRELEQQAIENATDRMSELCASMEMTPQEQQIVLGRAADQIHELAK
                     HRDADLIVIGSHGRHGWRRLLGSTANAVLHGAPVDVAVIRIPENQDT"
     gene            9677..10129
                     /locus_tag="AC16QF_14705"
     CDS             9677..10129
                     /locus_tag="AC16QF_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449858.1"
                     /translation="MTDNNSEGRISAFAKDVQRVAEEVEHLGEDTKLVGEEVVGAEIL
                     LTKELTEKIGIAWETAERAGAIGADQVRHLIEDYIATAREFSETPGLHNFLDLTLNHL
                     GRRITHVSEGARAVADLVAEESGRLEDAVLGVWKPFLAVLNRDWESRG"
     gene            10179..11603
                     /locus_tag="AC16QF_14710"
     CDS             10179..11603
                     /locus_tag="AC16QF_14710"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR6449859.1"
                     /translation="MKLLAIDKLQLPAFASTPDGRLVFVNAAWRERFAAGVGDPWHDP
                     FPKVEIDTIGEAWQACHLAHQPFMLEASSPSERYRILCQPVLENGQVLVLGTMADITQ
                     QSRQEAETEAILDTAVDAIIIIDETGRIETFNKAATQLFGYTAAETLGKPVSMLMPEP
                     HRSQHDHYMSRYLTTGEAKIIGIGRDLEAVDQSGRRIPIHLSVSEVQSESGRRFTGII
                     RDLSEQQAARQALAEQREKLAHVGRLSSMGEMTASIAHEINQPLTAISMYAQAGIKLI
                     DRGEPDLARLRDALEKLNTQSLRAGAVIERIQRFARAQKSERIMLDVNHLLDDLGKLA
                     ESDARLYNVELELELAPGLPEVFGDPIQVQQVALNLIRNAIDAMRDIRFVHGRRISLS
                     TSLRSDRMVEIAVSDLGPGVAEDQKELLFTPFHTTKKEGMGMGLSICRSIMTEHGGAL
                     SYRDNDGPGATFFFTLPTWRADDE"
     gene            11596..12219
                     /locus_tag="AC16QF_14715"
     CDS             11596..12219
                     /locus_tag="AC16QF_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6449860.1"
                     /translation="MSEHETTVFVVDDDEPVRDAIGLLLETVEIPYESFPTAQAFLDA
                     YDPSRTGCLVLDIRMPGMSGLELQERLLEDKAPIPIVFITGHGDIPMAVEAMKRGAID
                     FIRKPFRDQELLDRIHAALNADEDQRGAYDNLTTIRERVAALTPREHEVFERVAQGQA
                     NKVVAIELGISERTVEIHRSQVMQKTEARTLADLVRMKLTLDQADGP"
     gene            complement(12297..13337)
                     /locus_tag="AC16QF_14720"
     CDS             complement(12297..13337)
                     /locus_tag="AC16QF_14720"
                     /inference="COORDINATES: protein motif:HMM:NF024779.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein N-acyltransferase Lnb
                     domain-containing protein"
                     /protein_id="HIR6449861.1"
                     /translation="MHSTGKQNFDDLDAEGRIGLVLRRGIRWTVVGFASLWAAGAVYV
                     DGPLGMDDGNIWLAVAWLAVAAACLALIRIAWKKYLVWLVWFLVVLIPWTAKNPSNER
                     PWKPEWAKTGWVEFSDGALEFHNFRNFDYGADGSVVESWETRTVSIRNLRGMDYFHDA
                     FGGDLIAHPMLSFDFGADGYVALSVETRREVDEAYSKLGGLYKMFELQYLWGDERDFV
                     RVRTNVRDEPVYLYRTSFPREQALFVLLDSVRETNLLARQPRFYNVISANCTTSLLAQ
                     TVEFRNAPFDIRMLANGRFDELIYETGGILGDGLPFEELRERALINPVALYAHGDAAF
                     SQRIRADRVGFD"
     gene            13536..13886
                     /locus_tag="AC16QF_14725"
     CDS             13536..13886
                     /locus_tag="AC16QF_14725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6449862.1"
                     /translation="MNANVCVVDGDPAVRDSLATLMRLSGHEVSTFATGQEFLDAAEA
                     DTVGCVICEADLPDTTGLAVFGALTEKSLAAPFALLISQSDPARVAQAYRAGIRNVFP
                     KPLVHRRILSFVSR"
     gene            13900..14829
                     /locus_tag="AC16QF_14730"
     CDS             13900..14829
                     /locus_tag="AC16QF_14730"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6449863.1"
                     /translation="MSSNSTTVDSSMELKNILVALHDRESMGSAMRKAARLEHFTGAN
                     ITVVQTCWDVVSEEPTQHFPQEEIDTIASRMKEAELNNLSAELARYSERVAELNAEIL
                     WSKHHDDAVARFAIQSGSDLIVAPRHARGGVKRLLLPEELKLAARAHAPLLLTSASTW
                     REPTNVLVALDAGDRQHAALNSLLIEWGRTLAETLGGELHLVAASPPPSSSAPNQTLE
                     EFQREAEARRMDALRALADAHPHDYGSLQVKTGDVCDVIDEVASACEVDIVVMGTAAR
                     TGLKRLFIGNSAESVLSQLTEIDILTVPDSQQS"
     gene            complement(14842..15210)
                     /locus_tag="AC16QF_14735"
     CDS             complement(14842..15210)
                     /locus_tag="AC16QF_14735"
                     /inference="COORDINATES: protein motif:HMM:NF048679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI/CycH"
                     /protein_id="HIR6449864.1"
                     /translation="MDEAVGRVVAQPMRPGEPVGPAAVVASVSYEGDVAGLPKTAAVF
                     VFARRSGERMPVAVERLAPARLPVDVVFRHEAGAELEIVARVSLLGGAKLEEGDVESA
                     VTVVAPGEGEKVIALRLPGI"
     gene            15456..>16854
                     /locus_tag="AC16QF_14740"
     CDS             15456..>16854
                     /locus_tag="AC16QF_14740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR6449865.1"
                     /translation="MTRIEKRTFCRVCEPACGLIATVEDGALTALRGDADHPVSKGFV
                     CNKGIYGLDIHNDPDRLRTPLKRTANGFEPIGWEQALEEIAGKLRRILDNHGTSALAT
                     YTGNPNAFNALYGPSFGNFARQLGVRKNFSSGTQDCSNKFAGSEAVFGTRTMHPLPDI
                     DHSDFILILGENPAVSHMSFCSIPNPMQHLKEAEQRGARILYVNPREIESARMAGSVL
                     RAKPDTDAFLLAAMIHEIYASRGFHDDVSRHGSHAEGLIEFIRQYDPERVAPITGIPA
                     ETIRQLARDFADAPSACAHMSTGVNMGRLGTMAYWLLHMLVLVTGNLGRRGGNFYSLG
                     FYERSPSAGRNVPEGHLETPFGAVRKPGGVGISLPGNLLASYLADPDEPVRAMFVNAG
                     NPVLSIAGEAATRQALENLELLVCVDIYRNATGEYAHYVLPAAGAFEREDINITGLGM
                     QFQPSVQFTEAVVPPA"
BASE COUNT         3267 a         5568 c         5125 g         2894 t
ORIGIN      
        1 ccaacccgga tccggccctc gacatcccgg acgatctcga ccacgtccag atagagctcc
       61 ggagagaacg cccggttgca gcggagttcc tcctcgcaat agtgtttcct gagacgagcg
      121 gtgctgaaat ccacaaagct gaagaggacc ggtttcttga ctttgtaggc gtaatctccc
      181 gtgaggaaga cccaggagat gtgcgtctcg accaggcgaa cggctctaca ggggtgcggg
      241 taagcctcgg gacgtagcag atgctcgacg agctcgggct gggccactat caacacctcg
      301 ccggagcggg gctgacgtcc gagcctgccg ccagatgctc actaggggaa ctacggatag
      361 gcaaatcgtc gccgaaaacc gttagcattg acacgaccat gccatgccgg gaaaccctct
      421 tgacgccagc taaacccatt aaggccacgt tgatcggctt gtttgccgtc tgcccgctca
      481 tctctccgat ggtagcccac cccaatacgg cagaacagga aaagacgggc aaggagctct
      541 acgagcagca gtgcgcccgc tgccaccagc gcgacggggg cggcgtagac gggctctatc
      601 cgagcctgag aaatgctccc tccctttggc tcgaccggaa aacggcgatt cgtggggtgc
      661 ttgccggacg cgctgacgac accgtggtca attcccccct catgccgacc cacggctatc
      721 tcggcaacga gaccgtcgcc agtaccctga cgttcctgct gcaggaatgg gggccggggg
      781 ggcagcctta caccgccgag gaggtcgccg ctgagcgact cgagctgctg gcaagccaac
      841 cggcccccca cccgggactg cccgatgtga gcccgctggc cgatatggac gcggtgcagt
      901 acgtgacgtc gagcggccct cccatgtccg tgagcgagtt cgaccgggcg cgatctctgt
      961 actacgggca ctgcacgggt tgtcacggcg tgctccgcga aggtactgcg ggatcgccat
     1021 tgacgccggt gctgatgcgc caacgcggga ccgaatacct tcaggcggtc atcaactacg
     1081 gttcatccgc gggaatgttc aactggggca cctcggccgc gctgacagcc gaggagatca
     1141 acatgctcgc tcgctttctg cagcacccgg tgcctcaggc tcccgacatg accgaggcag
     1201 acgtgcgcgc gagctggtct ttggaggtac ctctaaagga ccgtccaggg gtgccccagg
     1261 ggcgccatcc gctggatgag ctgttcgtgg tgaccctgca cgatgttgct cagatagcgc
     1321 tgatccacgg tccttcgcga cgcgtcatcg gcacggtggc aacgggccag agcccgcacc
     1381 gggttcgcgg atccgccagc ggccgctatc tctacgtgat cgcccgggac ggatcgctca
     1441 ccatgatcga cctgtacgcc aggaagccgc gcagggtggc cagcgtaagg gtcggctacg
     1501 aagcgagagc cgttggcgct tcacgccatc ccgattacgc ggatcgttac gtccttgccg
     1561 gcgcctactg gcctcctcag ctcgtgctcc tggacggccg cacgcttgaa cccctgaagc
     1621 tgatctccac ccgaggccga acggccgatg gcggccgcta tcatccggag ccacgagtca
     1681 gcgacatcgc aggctcatac ctgcgccccg agttcatcgc caacgtgaag gagacgggcc
     1741 gggtctacct gtttccctac tacgccaccg accgcctgca gctggtggac atcggcacca
     1801 cggcggaact cagggcgggc acgttttctc aggacaagcg ctacttcatc acgccggccg
     1861 acgtcaacgc catcagcgtt gtagacacca aggaacagcg ccaggtggca cagatacccg
     1921 cccgggtgtt tgccggcggg cccggcgtga cttacgacga tccggagtac gggcccgtct
     1981 ggtccacgag caccatggtc gaggacgaga tcatcagcat cggcaccgat cccgagggtc
     2041 accccaacta cgcctgggag aaggtgagat cgctgaaagg acccgggtcc ggctcgatgt
     2101 tcatggcaag ccatcctgca tccaccaacc tctgggtcga ttcaccgctc aacgctgacg
     2161 agaccgtcag ccagtccgtc gccgtgttcg acaaacgcga cctggatgcc ggctaccgca
     2221 gcatcgacgt cgccgccatc gccgacgtcg gcgagggacc aagccgcgtc ctgcagccga
     2281 cctacgaccc gaccggccga gaggtgtggg tggtcgtctg gaacccccag gacctgaact
     2341 ccgccatcgt ggtcatcgat gaccgtaccc tgaagccgct ggctgtgata gacgatcccc
     2401 ggctgatcac gcccaccaga atctacagtc tcgccgcctt gcgggacgct ccatcggaga
     2461 actgaacgtg ctcaacactc tcaagctgat cctggccctg gcagcaacct gctacttcgg
     2521 tctggctgcc gccgacgaca tcgcctacga cggcggcgag ctctaccagg caaactgcgc
     2581 caactgtcac ggcatgtacg gcgagggcga cggcatcgtg acgccgtctc tgtcggtcgt
     2641 cctccaggat ctgcgctacc tggccgagcg caatgacggc gagtttcccc gctggttcgt
     2701 cacggagatc atcgatggcc gcgagctcag gcttgcccac ggaccagacg gcatgcccgt
     2761 ctggggagag gccttcaccc gcatggaagg ctacggagag caagcccaga gccgcgtcga
     2821 gcaaaaaatc gacgcgctgg tgaatttcat cgaatccatc cagatcgctc cggaagacga
     2881 atgacccgtc atgcctttgc ggacgcctcg gtcccggctg tttcatgacc ggatgagagc
     2941 aatactgccg gatcgttacc tggctacggg tggacggcca gcaagtcgca cggcgatttc
     3001 tcgagcgttc gctgagcgga attcccgatg acgacggtgc cacccttgcg ccgggtgctc
     3061 gtaccgatca cgagcagatc cgcatcgatc ttgcgcgcgg tgatcgcaat catctgaccg
     3121 accttgccca gcggcgtgtg catcctcgac ttgggcacat cgaacggctc cagcagcgcg
     3181 ttcatgagct cctggttgtt ggccagcgcc ttcttcttga cgctgcgcgg atcgatcagg
     3241 tcaaggtcgc tcaacacctg tgaaaactcc accacgttca cgcagtgcag cttgccgccc
     3301 gacatgtcgg caaactcctg ggccgcctcc agaacacgga tgttgagggc ctgatgcttc
     3361 ctgtcgcgat gacggaaatc cagggcggcg aggacattac ccgacgctga gcggcgcggc
     3421 ggcgccgata tgtagacagg cttggaacac tgctccagga gctgccagtc cagcggcgtg
     3481 tgaacgagcg tccgggagcg attgacggac ttgagcacca gatcaaactc gttggccgcc
     3541 gctttctcgc tgacccagcc tgcgatgttg tcggtccact gaacctcgat gctgacgtcc
     3601 gcggctgcca tacctgcatc gcggacctga tcgatcagcc aagactcacg ctcacgcatg
     3661 atcgtagctt tgatcgcacg gcgctgatgc gcctcgaaca cgtccgactg ctcgcacatg
     3721 gcgttccagc agaaggagac cagcctcagc cgggcgccac aactctgctg cagctgcttg
     3781 gccctctcca gagcaaactg tttgtgcttg ggcttcgcca tcaccaccat ggccgacctg
     3841 atcttcaaac ccgttctccc gtcgatcgct ttcgtttaat cggtaccatg catggtcagc
     3901 acgtccgtgg caaccgcatg aagtatgcgc tccgaggtgt tgcccagaag cagcccccgc
     3961 acgccttccc gggcgtgggt acctacgatg acgagttcag ccccggttag ctcggcaacc
     4021 gccccgatgg tgtgggtggg atacccctcc tcggcatgca gcatggcaaa cttcacgccc
     4081 gctcgggcag cgatggcgtc gacgcggcgg atgatctcgc gctcgacatt ctccttgatt
     4141 tccgcgtagc ttctgaccgc cccgagctca cccacccagg gctcgaacag cggataagcg
     4201 cagaccacgt gaagctcgcc gcccagcatg tccgccagcg cccggccctc gcgcagcatc
     4261 gccgcgttca ggttggcgtg agcatcgtcg aaagggtcca ccgcgcagag caggacgggc
     4321 tcgtcaaccc aggcctgggg cttgaccagc atcacgggca cgctggcgtg gcgcagcaga
     4381 ttccagtcgt cgggggtacg caccacgtct ccgaatttct cgtgaagggt ggccggcttg
     4441 acgatgaggt ctgcctccac ggcctcagcg gcatgcagga cgccctccca ggcgttgcga
     4501 ttccaaagag tcgcgcattc gatcgccggc agctgggcac gcagcgggtc cacgagctcc
     4561 tcaagcgccg actcggcgga ttcgagaatg agcgttttca gacgtgccga gtcctcgatc
     4621 tcgtcgatcg tcagttcggc tatgccttcg taaacgacgc tcaccacatg cactttcgcc
     4681 tgaccggcct tcgcgagtat caggaccttc tccaggacga accgcgcctc ggccgtgtcg
     4741 ctgagcgcca cgaggatgtt gttgatagaa tccattgctc gaaccccacc ggcagattca
     4801 ggtggaattg atcataggtg gcgggctcgc cgcggcgtat ccgtagtttc cactggcgct
     4861 tcggtatgta cgtactgacc cgcgcccgga ggcggcgtga aatgggttca tcgacatgaa
     4921 acagatcaac aacatactcg tagccggcga ctcgctgaac ggcatggaag ctgccctggc
     4981 caaggcagcg aagatcgagc actacaccgg cgcgaacgtg tgggtcgccg tggtgattta
     5041 cgacgccatc gccgaggaag atccggacag gctgcctccc gagcagcaat cacaactcgt
     5101 cgaggcgctc aaggcagcgg aaagaaacgg ccttcgtcag ctgacggaac cgtaccggtc
     5161 aaggatcgcc acgctcgatg accgggtcgt ctggaacaag aacgcggccg aagggctgct
     5221 ccgggttcag accgaggtcc atgcggatct catcatcaag ccgatttccg agcatggctt
     5281 catcaccgac catctccaca caccgctgga ctgggcgttg atgcggggat cccagggcgc
     5341 ggtgctgata tcgaaaaagt cggactgggc caacccccag cgggtcctgg ccgccatcga
     5401 cgttgccgac acccgccatg ctgacctcag ccgcgaaatt cttctgaccg cagcgaccat
     5461 ggcccggact ctggacgcgg agctgcacgt tgtgaccgcc ttcccgagcc tgggccagac
     5521 ggtcaacgac cttcaggtag ccatggacta cgaagggatc aagcgcgaca tgcgtgagaa
     5581 ccggaccgcc gcgatcgccc gatggatcga ggaactcgac atcgggatca gcgactcaca
     5641 cgtgctggag ggcaagcccg aactcgtgat accccagttg gcaagcgatc tctccgcgac
     5701 gctcaccgtc ctgggcaccg ccgcgcgaca gggcatcagg aaactgctaa tcgggaacac
     5761 cgcggaaaac atcatcgccc ggctcgattg cgacattctc accgtacgcg agccgtgaga
     5821 acttcgtcga gcgttcagtc cgattgaccc gacttcggca cgccgctgtg ctccagtatt
     5881 cgctcgatgg actgcatgac gttcatcggc aacgggaaga cgatggtgga gcttttctct
     5941 ccggcgatct ccgtcagcgt ctgcaggtag cgcagctgca tggcttctcc ctgtgaggcg
     6001 agaagctgag cagcctccac cagcttcgag gcggcctgcg cttcgccctc cgcgtgaatg
     6061 accttggctc ggcgttctct ctcggcttcc gcctgcttgg caattgcccg gatcatgctc
     6121 tcgtcgatgt cgacgtgctt gatctccacg ttggcgacct tgatgcccca ctgatcggtt
     6181 tgcttgtcca ggatcgcctg gacgtccgag ttgagccgct cccgctccgc cagcagctcg
     6241 tcgagctcat gctggccgag cactgaccgg agcgtggtct gggcgagttg cccggtcgct
     6301 tccatgtagt cttcgacgtt gatgatcgac cgctccggat cgacgacccg gaaatagaga
     6361 acggcgttga ccttgacgga cacgttgtcg cgggtgatga gatcctgcga ggggatgtcg
     6421 agcacaatgg ttctcaagtc cacccgaacc atctgctgaa tgacaggaac gatgatgatc
     6481 agaccgggcc ccttgacctt gtagaagcgt ccgagcatga agacgatgcc ccgctcgtac
     6541 tcccgcagga cccggaaagc gctgatcaga agaccaacga tgatgaccgc gatgactgtc
     6601 gaaaatattt ccattaatca atcctccggt tgaacgacca gaatcagccc gtctacttcg
     6661 gttacgcgca ctttcgcgtg tgccgccacg ggctcgctgc agcgtgcctc ccagatctcg
     6721 ccgaatgctc gaacgtggcc cgtcccctga aagtcctcca gcgcttctcc cgaggcgccg
     6781 atcattgctt ccacgccggt tacgatcttc gcccgacggg ccttcatcgc agccccgatg
     6841 gcaaaaatcg tcaggcccgc actggctgcc gcgaatgcgg ccaccacggt aatcgggatg
     6901 ccgtagccgg gcgcctcggt gtccatcagg atcatcgagc ccgcgacgaa cgcgacgacc
     6961 ccggcgaggc ccagcacacc catggttggc gtgaagacct ccgcgatcat catgccgatg
     7021 ccgaccacga tcagcgccag gcccgcgtag ttgaccggaa gcatctgcag accgtatgcg
     7081 cccagaagca ggcagatgat tccgatcacc gagggaaaga ccatcccggg attgtagaat
     7141 tccagtatca ggccgtagac gccgaagatc agcaggccgt aggcaatcgt cggatccgtg
     7201 atcacttcga gaagctcgtg ctgccagtcg gtctccacgg tgtgcacgcg cgcgtttgcc
     7261 acatcgagcg tgacggtccg attgggaagc tccaccaccc acccgtcgat gtcggcaaga
     7321 agcgaggaga cgtcccgcgt gatgagatcc accacgttgg cttccagagc ttccgaggcg
     7381 cgcaggttcg cgccaagccg caccgtctcc tcggcccaat cgatattccg gcccctgagc
     7441 tccgccagcc cctgcagata ggctacggcg tcgttcacga ccttgcgtgt catggcgtcc
     7501 cccgacggtg cccgcccggc gtcgccttcc gtttcgccgg cgtcacgcgg gtcgggcatc
     7561 tgaggcgtcg gcgcgatgct gacgggcgta gaggaaccga tgttggtagc cggtgccatg
     7621 gctgcgatgt ggctggcgta agcaatgtag gttccggcgc ttgccgcacg ggcgccgtcc
     7681 ggcccgacga aggtgaccac gggcaggtct gccgaaagca tctccttgat cagcccgcgc
     7741 atggccttgt ccagccctcc cggtgtgtcc attcgaatca ccagcgcgcg ggccccggcc
     7801 tcctgggcat catcgagcga gcggatgatg agatccgccg tggcaggacc gaggggaccg
     7861 tcgagatcca ccagccacac cacaccgtcc gccgcgggct gcgccccggt ggctggacgg
     7921 gcttccccga agcccagggc aagtccggca caagcgatga gaatcggtag ccaggccttc
     7981 atggtgggag cagcaatgtc caagttcccg aacgttgcag cattgtacgc cttattgccg
     8041 ggatcggctg atcgaactcg cccacggcct agccgcgggc aatgagcgga cctaaccttc
     8101 tgccggccgc acccgcacgg tcagcacatc gcattcggcg ccgtgcagca ctgcgttggc
     8161 cgtggaacca agcagcacgc cgggcacccc gtggcgaccg tgagacccga tgacgatcag
     8221 gtcggctccg atcttcgcgg cgtaagcgcg aatttcgacc gccggcctgc cgtagagtac
     8281 ggcaacgcgg tccggcgtga tgccgaaccg cgccacacgc tcacgcacgt ggctggagat
     8341 atcctttgtg atggtttcct ccatctcgcg cagcggccag gtggcagcga acgatgcgcc
     8401 atccatcccg ctcacaccgc ccatgaccgg aggaatgacc gtgcagacct gaaagcgggc
     8461 tgccccgcga ggatcgaggg tggtcgcggc ctccagcacc tcgtcagcct cgtcggacgc
     8521 gtcgatcgca atcaggacgt ttctgtagct gatcgccata gccgccgccg ctcaggtgat
     8581 acggacggtc aggacgtccg tttccgcgtg gtgcagcaca ccgttggccg tggacccgag
     8641 caggagacca agaccgtgct ttccgtggct gccaacgacg atgagatcgg ctccgtgagc
     8701 ctcagcggcc gcgacgatct gccccgacgg ggagcccacc atcacgtgca cgtccgcagc
     8761 ctccaagccg gcgtcagcgg ccatctcgcg ggctttggaa agcgccaggc tgcgcgcttc
     8821 ttcttcgaag tcgacggaag cctgggtgaa ggagaccatg tccagaccac cgtagacatg
     8881 cgtcaggggc ttgacgaccg tcacgatgga gagcttcgct tcatgctcgg acgctacttc
     8941 ccgggcctga tcgaggacct gtcgggcttc atccgtcatg tcgactgccg ccagcacgtg
     9001 cttgtatgac atttagaggc acctttgatg aaactccgga caatctactc gcgttgggac
     9061 cagggccaca tcgggacatg tcccctggag gtttcgggtt ccccttagta ggaatcggtc
     9121 ggcatttgcg cgatggttct ggaggctccg tggatgcaag ttgagccttt ggtaggatga
     9181 agcccgcatc cgctcaccgg ggcagaccct caatcatcgg ggatcaggat ttgtacaaga
     9241 acatactcgt tgccatcgac ctcatggaag gtcctgccca tcaggtgctg tccggcgcgc
     9301 tggcgttcgg agaggaaacc gctcaataca cggtcgttca cgtcgtcgag cctcaatacg
     9361 tgcagtacag cttcgacccg acgttcaccg gatcactcac ccgcgagctc gagcagcagg
     9421 ccatcgaaaa cgcaaccgat cggatgtccg agctgtgcgc ctccatggag atgacgcctc
     9481 aggagcagca gatcgtgctg ggacgagccg cggatcagat ccacgagctg gcaaagcacc
     9541 gcgatgccga tctcatcgtc atcggctctc acggaaggca cggctggcgc cgcctgctcg
     9601 ggtccacggc caatgcggtg ctgcacggtg cgcccgtgga cgtggccgtc atccgcatcc
     9661 cggagaatca agacacatga cggacaacaa cagtgaaggt cgcatatcag cgttcgcaaa
     9721 ggacgtgcag cgtgtcgccg aggaggtgga acatctcggc gaggatacga aactggtcgg
     9781 cgaggaagtc gttggcgcgg aaatcctgct gaccaaggag ctgaccgaga agatcggcat
     9841 tgcctgggag acggccgagc gcgccggggc cattggcgcc gatcaggtcc ggcacctcat
     9901 cgaggactac atcgcaaccg cccgggaatt ttcggaaact cccgggctcc acaacttcct
     9961 ggatctcacc ctcaaccacc tgggaagacg gattacccat gtaagcgaag gcgccagggc
    10021 cgtcgccgat cttgttgccg aggaatcggg acgcctggag gacgcggtgc tgggcgtgtg
    10081 gaagccgttt ctcgcggtgc tcaatcggga ctgggagtcg cgcggctagg cggatccgac
    10141 atgggcagca ccgcaaagct agccatgtcc gcggcaacgt gaagctgctg gcaatcgaca
    10201 agctccagct tccggcgttc gcgagcacgc ccgatggccg cctggtgttc gtgaacgccg
    10261 cgtggcggga acggttcgcc gcgggcgtgg gagatccgtg gcacgatccg ttccccaagg
    10321 ttgagatcga caccatcggc gaagcgtggc aggcatgcca tcttgctcat cagcccttca
    10381 tgctcgaggc cagctcgcct tcggagcgct atcgcatcct ttgccagccg gtcctggaga
    10441 acgggcaggt tctcgttctc ggcaccatgg cggacattac ccagcagtcc cgccaggaag
    10501 cggagacgga ggcgattctc gacaccgccg tcgacgcgat catcatcatc gacgagacgg
    10561 gccggataga gaccttcaac aaggcggcca cgcagctgtt cggctacacg gccgctgaga
    10621 ccctcggcaa gcccgtcagc atgctgatgc ccgagcccca tcgatcgcag cacgatcact
    10681 acatgagccg gtacctgacg acgggcgagg cgaagatcat cggcatcggg cgtgatctgg
    10741 aagccgtgga ccaaagcggc aggcggatac ccattcacct ctcggtgtcg gaggtgcaat
    10801 ctgagagcgg caggaggttc accggcatca tccgcgatct ttccgagcag caggcggcgc
    10861 gtcaggcgct cgccgagcag cgcgaaaagc tggcgcacgt cggtcggctc agctccatgg
    10921 gggagatgac ggcaagcatc gcccatgaga tcaaccaacc gctcaccgcg atctcgatgt
    10981 acgcccaggc aggcatcaag ctcatcgacc ggggagagcc ggacctcgcc cggctgcgcg
    11041 acgctctcga gaagctcaac acccagtcct tgagagccgg agccgtcatc gaacgcattc
    11101 aacggttcgc acgcgcgcag aaaagcgagc ggatcatgct ggacgtcaat catctgctcg
    11161 acgatctcgg caagcttgcc gagagcgacg ccagactcta caacgtcgag ctggagctcg
    11221 agcttgctcc cggtctgccc gaagtcttcg gcgatccgat tcaggttcag caggtcgcct
    11281 tgaacctcat ccggaacgcc atcgacgcca tgcgggacat tcggttcgtt cacggccggc
    11341 gcataagcct gagcacgtcg cttcgaagcg atcgcatggt ggagatcgcc gtgtcagacc
    11401 tcggcccagg ggttgcagaa gaccagaagg aactgctatt tactcccttc catacgacca
    11461 agaaggaagg aatgggtatg ggtttatcga tctgccggtc gatcatgacc gagcacggtg
    11521 gcgccctgag ctatcgcgac aacgacggac ccggagcgac cttcttcttc acgctgccca
    11581 catggcgcgc ggacgatgag tgagcacgaa accacggttt tcgtcgtcga cgacgacgag
    11641 ccggttcgcg acgccatcgg gttgctgctc gaaaccgtcg aaattcccta cgaatccttc
    11701 cctacggccc aggctttcct ggacgcctac gatccgtccc gaaccggttg cctggtgctc
    11761 gacatccgca tgcccggcat gagcgggctg gagctccagg agcgcctgct ggaggacaaa
    11821 gccccgatcc cgatcgtttt catcaccggc cacggcgaca ttcccatggc cgtcgaggcc
    11881 atgaagcggg gcgcgatcga tttcattcgc aaaccctttc gggaccagga gctgctcgat
    11941 cgcatacatg cggcgctcaa cgcggacgaa gatcagcgcg gcgcgtacga caacctgacg
    12001 accatccggg agagggtcgc ggcgctcact ccgcgcgagc acgaggtatt cgaacgggtg
    12061 gcccagggcc aggcaaacaa ggtcgtcgcc atcgagctgg gcatcagcga gcgaaccgtg
    12121 gagattcatc gaagccaggt catgcagaag accgaagctc gaaccctggc cgacctggtg
    12181 cgtatgaagc tgaccctgga ccaggccgac gggccgtagc cggcagcagc tgaccggaga
    12241 tcccggcccg gcgaccatcg cggagagccg ttggagcgcg gggttccccg aagggactag
    12301 tcgaacccga cccgatctgc tcggatgcgc tgcgagaacg ccgcatcgcc gtgcgcgtag
    12361 agcgccaccg ggttgatcag cgcacgctcc ctgagctctt caaacggcag gccgtctccc
    12421 agaataccgc cggtttcgta tatcagctcg tcaaagcgtc cattggcgag cattcggatg
    12481 tcgaacgggg cattgcgaaa ctccacggtc tgcgcgagca ggctggtggt gcagttggcg
    12541 ctgatcacgt tgtagaagcg cggctggcgt gcgagcaggt tggtctcccg aaccgagtcg
    12601 agaagaacga acagcgcctg ctcgcgcggg aagctggtgc ggtacagata cacgggctca
    12661 tcacgtacgt tggtccgtac ccggacgaag tcgcgctcat cgccccacag gtactgcagc
    12721 tcgaacatct tgtagagccc gccgagcttc gagtaggcct catcgacctc gcggcgcgtt
    12781 tccacggaca acgcaacgta accgtcagct ccgaaatcga aggagagcat ggggtgggct
    12841 atcaggtcac cgccgaacgc atcgtgaaag taatccatgc cgcgaagatt gcgaatactg
    12901 accgtgcggg tttcccagga ctccaccacc gaaccgtcgg cgccgtagtc gaagtttcgg
    12961 aaattgtgaa actcgagcgc accgtcgctg aactccaccc atccggtctt ggcccactcg
    13021 ggtttccacg gcctctcgtt ggaagggttc ttggccgtcc agggaatcag caccactaga
    13081 aaccagacca gccagacgag atatttcttc caggcaatcc ggatcagagc caggcacgcc
    13141 gcggcgaccg caagccacgc aacagccagc cagatgttgc cgtcgtccat gcctaacggt
    13201 ccgtccacgt aaacggcgcc cgcagcccac aggctggcaa acccgaccac cgtccacctg
    13261 attccccttc tgagcacgag cccgatacgg ccctcggcat ccaaatcatc gaagttctgc
    13321 ttaccggtcg aatgcatctg ttcggaaaga ctcaccatct cgaaaacaat gcgtccctcg
    13381 cgggaaggac accaagccgc cgcgccggat tctaccaggg tcaacggccg ggcccgaacg
    13441 cctccgggcg atgcgttagg tacatatccg tatttgcagg tatcggcccg cgccataagt
    13501 tatcggccca gagtcattct tcgtttcgga ttgtcatgaa cgctaacgtc tgcgtcgtcg
    13561 acggcgaccc cgccgtacgt gacagcctgg ccacattgat gcggttgagt ggtcacgaag
    13621 tgtcgacctt tgccaccggg caggagtttc tggacgccgc agaagccgat accgtgggct
    13681 gcgtcatctg cgaggccgac ctcccggaca ccacggggct ggccgtgttc ggcgcgctga
    13741 ccgaaaagag cctggcagca ccctttgccc tgctgatcag ccagagcgac cccgcccgag
    13801 tagcacaagc ctatcgcgcg ggcattcgca acgtgtttcc gaagccgctc gtccatcgcc
    13861 ggatactgtc cttcgtctcg agatgatggg ctggcacaat tgtcttccaa ctcgaccacg
    13921 gtggattcgt ccatggagct gaagaacata ctggtggccc tgcacgatcg ggaatcgatg
    13981 ggaagcgcga tgaggaaggc cgcccgcctg gagcacttca ccggggcaaa catcaccgtg
    14041 gttcagacct gctgggacgt ggtcagcgag gagcccaccc agcacttccc gcaggaagag
    14101 atcgacacca tcgcctcgcg catgaaggag gcggagctca acaacctgtc ggccgagctc
    14161 gcgcgctaca gcgaacgggt cgcggagctg aatgcggaga tcctgtggtc aaagcaccat
    14221 gacgatgccg tggcccgctt cgccattcaa tcgggatccg acctcatcgt cgctccccga
    14281 cacgcccgcg gcggtgtgaa gcgcctgctg ttgcccgaag agctcaagct cgccgcgcgc
    14341 gctcatgctc cgctcctgct gacttccgct tcgacctggc gcgaaccgac caacgtgctg
    14401 gtcgcgctgg acgccgggga ccgtcagcac gcagccctga acagcctgct catcgagtgg
    14461 ggcaggaccc tcgcggaaac tctggggggc gagcttcacc tggtggcggc gagcccgccg
    14521 ccgagctcga gcgcgcccaa ccagacgctg gaggagttcc agcgagaggc ggaagcacga
    14581 cggatggacg cgctgcgcgc cctggccgat gctcatcccc acgactacgg gtccctgcag
    14641 gtcaaaaccg gagacgtctg cgacgtcatc gacgaggttg ccagcgcatg cgaggtggac
    14701 atcgtcgtga tgggcaccgc cgcgcgcacg ggattgaagc ggctgttcat cggcaactcg
    14761 gcggaatccg tgctcagcca gctgaccgaa atcgacatcc tcacggtgcc cgattcccag
    14821 cagagctgat ccgcgcgcct gctaaatccc aggcaaacga agcgcgatca ccttctcgcc
    14881 ctccccgggc gcgacaacgg tcaccgccga ctccacatcg ccctcctcga gctttgcgcc
    14941 ccccagcagg ctcaccctgg ccacgatctc gagctccgca cccgcctcat ggcggaacac
    15001 cacgtccacc ggcagacgtg ccggcgcgag ccgttccacc gcgaccggca tgcgctcgcc
    15061 agatcggcga gcgaagacga acacagccgc cgttttcggc agcccggcaa catccccctc
    15121 atagctgacc gaagctacca cggctgcagg gcccacaggc tcgccgggcc gcatcggctg
    15181 ggccaccacc cggccgacag cttcgtccac atgggctgct gatgcaggcg atccgccaac
    15241 gccacgctca tctgacgaat accgttgcgc gaccacggtc gtgatggcga tcgtcggtaa
    15301 gcagcacaga gcaacaattg ccagattgcg ccggttcaac gtggtctcga tattgaggaa
    15361 gaagccacat tctcgacgcg tcccgccgca ccggcaagac ctcacgcagc aagcggcgct
    15421 gagctatgat cggatcagcg attcggaggg ttacggtgac acgcatcgag aaacgcacgt
    15481 tctgcagagt ctgcgagccc gcctgcggcc tgatcgccac cgtcgaggac ggtgcgctga
    15541 ccgcgctgcg cggcgacgcg gaccaccctg tctcgaaggg cttcgtctgc aacaagggca
    15601 tctatgggct ggacatccac aacgatccgg accgcttgcg cactccgctc aaacgcaccg
    15661 ccaacggctt cgagcccatc ggctgggagc aggctctcga ggagatcgcc gggaagctgc
    15721 gccgcatcct ggacaaccac ggaacatcgg ccctggcgac gtacacggga aacccgaacg
    15781 ccttcaatgc gctgtacggg ccctcgttcg gaaacttcgc gcggcagctg ggcgtgcgga
    15841 agaacttcag ctccggcacc caggactgca gcaacaagtt cgcgggcagc gaggccgtct
    15901 tcggcacccg aaccatgcac ccgctgcccg acatcgacca ctccgacttc atcctgatcc
    15961 tcggcgagaa tccggccgtc agccacatgt ccttctgctc cattcccaac cccatgcagc
    16021 acctgaagga ggccgagcag cgcggcgccc ggatactgta tgtgaacccc cgggaaatcg
    16081 agtccgcccg catggccggc tcggtgctca gggcaaagcc cgacaccgac gcgttcctgc
    16141 tggccgcgat gattcacgag atatacgcat cacgcgggtt tcacgatgat gtgtcccgtc
    16201 acggcagcca tgccgaaggc cttatcgagt tcatccggca atatgatccc gagcgggtcg
    16261 ctccgatcac cggcattccc gccgagacca tccgccagct cgcacgcgac ttcgccgacg
    16321 ccccgagcgc ctgcgcccac atgtccacgg gggtcaacat gggccggctc ggcaccatgg
    16381 cctactggct gctgcacatg ctcgtgctcg ttaccggcaa tctcggtcgc cgcggcggca
    16441 acttctactc tctgggattc tacgagcgca gcccttcggc cggccggaac gtccccgagg
    16501 gccatctgga aacgcctttc ggggcggtac gcaaaccggg aggggtgggc atcagcctgc
    16561 cgggcaatct gctcgccagc tatctggcgg atcccgacga gcccgtacgc gccatgttcg
    16621 tcaacgcggg taatccggtg ttgtccattg ccggtgaggc cgccacgcga caggcgctgg
    16681 agaacctgga gctgctcgtc tgcgtggaca tttaccggaa cgcgacgggg gagtacgccc
    16741 actacgtgct gcccgccgcc ggagccttcg agcgtgaaga catcaacatc acggggctgg
    16801 ggatgcagtt tcagcccagc gtgcagttca ccgaggccgt cgtcccaccg gcct
//
LOCUS       DBNHLM010000322        35050 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1365458, whole genome shotgun sequence.
ACCESSION   DBNHLM010000322 DBNHLM010000000
VERSION     DBNHLM010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 35050)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 35050)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35050
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1365458"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..3697)
                     /locus_tag="AC16QF_14745"
     CDS             complement(140..3697)
                     /locus_tag="AC16QF_14745"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/M1 family
                     aminopeptidase"
                     /protein_id="HIR6449866.1"
                     /translation="MFLEVFRFESRFQVRSPLFLTISTVWFLFGFLITGSESVSVGGV
                     GSNLNLNASFAIIQTITVLTIIGMFAAIAFVASAITRDRETKTAEIFYSSGISERSYL
                     LGRFAGGCVFATAAVTAGVVGTMLGTFMPWLDAERIGSFSLAPYVFSMLVIVLPNMFI
                     ICALFFSVAALTRSMMASYVAALGFLVSFAVIGSATDPENIRYLALGEPFGQVALGEI
                     TRYWTVFDRNFRVPELAGTLLLNRVIWVLVATAVLGITVARFAFELKPARKRWWRRRP
                     NRSEARAAPRHVVDVARASAGMTASLLSQLRMDFRGIIRSVPFYVLLAFGMLNVIGGF
                     YGAVSQWYGTEVYPVTRIMTQVVGSTFAFVVLLILIYYSGELVHRERQSGVADYLDAA
                     PYPNGIMILSKVFGLWLVISLLYLVVVLAGMLVQALNGYREFEPLLYLVSVFGVIGGS
                     FYLWAVPAVLIQSLASNKFLGMAALLVIFLGLTTLEGFGFEHMLYNFGLPAAPYSDMN
                     GYGHFVGRIVSAFAYWASLCVILVVVAHLFYPRGLQTAAERLATARQRFSGAVGAVVV
                     LAALTFCGLGGWIFYNTNVLNEYLTSEDLEEAQADYEKAYRQHRRLPPLQVVDLNLAV
                     DVFPDERRLESRGTARLINANEQPVSDVHLTLPPLLRVNELSLERAGLVAEDERLGYY
                     QYVLDEALPPGAETTLSWDLSWINEGFPNSGATTRVVGNGTFVDSTEIAPTLGYDSGR
                     ELNDNNVRREYGLPPVERLPKLGDPDWLGTNQLGVSVRSDFRAVVSTSADQIAVAPGY
                     LQREWVEDDRRFFEYAMDQPIWPFVSVSSARYVVARDKWDDVDLEVYHDPKHPYNVET
                     MITATKKSLDYFTREFSPYQYRQFRILEFPAYASFAQSFPNTIPYSEGIGFIADLRDP
                     AEIDYVFYVTAHELAHQWWAHQVVGAQMQGMTVIVETLAQYSALMVMEQEYGPERMRR
                     FLKYELDNYLQSRGGELIEELPLLYVENQGYIHYSKGSVVMYALKDAIGEDKVNAALK
                     SFIAKYGFKEGLFPTSRDLLDEFRAVAGAEHQPLITDLFERITVYDLAVTDASVAEND
                     GGFDVTITVAARKLDADGEGREAEVPLSQMFDLAVFPEAPEDLSEDDLPDPLLAERRQ
                     VVSGEQTFTVRVDERPERVGIDPYNKMIDRNPDDNLRRL"
     gene            complement(3709..4596)
                     /locus_tag="AC16QF_14750"
     CDS             complement(3709..4596)
                     /locus_tag="AC16QF_14750"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017737462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:0140657 - ATP-dependent activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6449867.1"
                     /translation="MLKINALSKTYPNGVQALDGVDLEIAKGMFGLLGPNGAGKSTLM
                     RTIATLQSPDSGSIHFNDVDVLADPNALRRVLGYLPQEFGAYPRTSPEVMLDHIAALK
                     GIGGRGARKALVEQLLVQTNLWDVRKKSIDSFSGGMRQRFGIAQALIGDPQLVIVDEP
                     TAGLDPAERRRFHNLLARIGEEVVVILSTHIVEDVAELCPNMAVMGGGRILVQGEPKE
                     LTGRLAGRLWSKVVPRDEAEGLRRSLPVVSTRLVAGQVELRVLSEQDPEGGLAAVEPS
                     LEDVYFATLMKHGMNPVVD"
     gene            4757..6190
                     /locus_tag="AC16QF_14755"
     CDS             4757..6190
                     /locus_tag="AC16QF_14755"
                     /inference="COORDINATES: protein motif:HMM:NF005686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6449868.1"
                     /translation="MSELARQAALDTAGMIRRGDASAVEVMDAHYDRIEILNPQINAL
                     VNVLPREAALELARQADRARSRGEPCGPLHGLPMAVKDTHDAAGFPTTWGFVPWAERV
                     ARHDGAVAARQRAAGALFIAKSNVPEFALGSHTFNNLFGTTSNPYDLSRTAGGSSGGA
                     AAALATGMLPLADGSDMGGSLRNPAGYCNVVGLRPSLGRVPADGPFGWFARLLTPGPM
                     ARNVPDAALLLSVQAGPNQDDPLSRPEPGRHFRDLPHADPATLKIAWSADLGFAPVDA
                     AMVRLLEAAAVVFEDLGCEVENACPDLRGGMSVFQTQRAASLAQTGRALDRSLPRWRE
                     HAKETMIWNVEQGYALTAEDLLRCEVERTAIYQRVGAFFAHHDALLLPSAQVPPFDKS
                     LDWVREIDGRAMETYIDWMTICCAVTVTDLPAISVPGGFTDDGLPLGLQIVGRPHGDL
                     DLLAIARLFEEATGHGQRRPPQSEHAG"
     gene            complement(6200..6382)
                     /locus_tag="AC16QF_14760"
     CDS             complement(6200..6382)
                     /locus_tag="AC16QF_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449869.1"
                     /translation="MYTVVDIASLQDYVLVEARRKAQEAGTVPIYRKFPVREVLDTLV
                     VDEEIVLFKPSLFPAL"
     gene            6549..7343
                     /locus_tag="AC16QF_14765"
     CDS             6549..7343
                     /locus_tag="AC16QF_14765"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR6449870.1"
                     /translation="MPESLRVMVVCAHPDDAEFHAGGLLVQLAAAGTDIHLLTLTDGS
                     AGHAELDRASLARRRAREAQAAARRLGARCTVWDTPDGELEPSLQLRGRLIVAVREFA
                     PHVLITHRSGDYHPDHRAAAQLVQDASFLLRVPNVQPDAPIMNEDPVILGFCDFFRRP
                     SPFRSDLLVDTEEQLEAVVELLACHESQVYEWLPHIMGLEVGENRRSWLREFYTQRPA
                     AVAHAHADDGARYAEAFELSEYGRQVTPQELAGLLAAGSAKANHSG"
     gene            complement(7346..7804)
                     /locus_tag="AC16QF_14770"
     CDS             complement(7346..7804)
                     /locus_tag="AC16QF_14770"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HIR6449871.1"
                     /translation="MRLQLALNVADIDEAVSYYSRILGVAANKREPGYANFVIEDPAL
                     KLVLFEAPGADERLNHLGFEAFADEDIEAVAGRLEDARVEHEVQTEEVCCFAKQNKVV
                     SYDPQGMMWEWYRVVEDSPTFFAEPEALACCAPAEPEAQGCCPPAERSAG"
     gene            7947..8246
                     /locus_tag="AC16QF_14775"
     CDS             7947..8246
                     /locus_tag="AC16QF_14775"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6449872.1"
                     /translation="MKLDAASACLESLGNSTRLAIYRALVRAGPAGLPVGKLQARLDI
                     PGSTLSHHIAHLVKNDLVSQTREGRVLRCTANYRTMNDLITYLSDECCRDTPRTT"
     gene            8246..8908
                     /locus_tag="AC16QF_14780"
     CDS             8246..8908
                     /locus_tag="AC16QF_14780"
                     /inference="COORDINATES: protein motif:HMM:NF012455.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HIR6449873.1"
                     /translation="MARRLAGEFMGTLLLLATVVGSGIMGEQLSGGNAAIALLGNTIA
                     TGAILVVIILMFAPVSGAHFNPAVTLTFLLRREIPAGAAAAYCVTQVAAGLAGVWLAH
                     GMFGLEIVQTSENSRSGFGQWLAEGVATFGLVATILAVLRSRPDLVAFAVGLYISAGY
                     WFTASTSFANPAVTIARAFTDTFSGIQPDHVPAFIAAQLVGAALATWTFGWLLSEGER
                     TN"
     gene            complement(8905..9555)
                     /locus_tag="AC16QF_14785"
     CDS             complement(8905..9555)
                     /locus_tag="AC16QF_14785"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF036807.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR6449874.1"
                     /translation="MTLKVIGAGFGRTGTLSLKLALERLGLEKCYHMLEVMQNEGHAA
                     VWRAAARGESVGWDGLFAGYQASVDWPSCNFWEAQLQHFADAKVILTRRDPERWYASV
                     MNTIYPSSVARREQADPAIREHMDMVFEVIWDGLFDGRMDDKEHVIAIYEAHGQHVID
                     ALPPEKLLVFEASAGWGPLCDFLDLPVPDEPYPWVNSTEDFRSRVAAASSKPQRDG"
     gene            complement(9578..10015)
                     /locus_tag="AC16QF_14790"
     CDS             complement(9578..10015)
                     /locus_tag="AC16QF_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HIR6449875.1"
                     /translation="MNGLRSIALALALVTSAAVLADGIRSPAPEGASLYFIAPADGAV
                     VSGPFTVKFGLKGMGVAPAGVDAPNTGHHHLLINLEEAVNPEVPLPATDQVRHFGGGQ
                     TETQLSLPAGTYRLQLMLGNYLHVPHDPPVMSELITVTVADQP"
     gene            complement(10037..10534)
                     /locus_tag="AC16QF_14795"
     CDS             complement(10037..10534)
                     /locus_tag="AC16QF_14795"
                     /inference="COORDINATES: protein motif:HMM:NF016001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR6449876.1"
                     /translation="MTIRDAVWRGFSLFVSLKPVTVFFMNLATWVDRPLMRATSGRIR
                     LSFVIPTLLLRCTGARSGLLREVPLLYVPEGDGALLVGSNGGKPYDPAWCHNLRAQRR
                     VSCLLAGREGTFRVEELSGADRDAAWESAVAIYPGYRRYEQRAGRVIPLFRLIPEADG
                     RARQT"
     gene            complement(10531..11535)
                     /locus_tag="AC16QF_14800"
     CDS             complement(10531..11535)
                     /locus_tag="AC16QF_14800"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6449877.1"
                     /translation="MARDQASWLNQVVEDVLEPDLPICDPHHHLWDHPGSRYLLDELL
                     ADTGSGHNVVATVFVECASMYRADGPEELRSLGETEFVNGVAAMSASGDYGPARVCAG
                     IVGYVDLTLGSSAGPVLDAHMAASDRFKGIRHAAGWDPSEKVRNSHTNPSEGLLLDDE
                     FRAGFAELGKRGLSFDAWLYHPQIPQLTDLARAFPHTTIIFDHFGGPLGIGPYEGRQD
                     QIFAAWQRDVAALAECHNVYAKLGGLVMPVNGFGFHKRERPATSDEIVAATGRYYRHA
                     IDCFGVDRCMFESNFPVDKQSCSYHVLWNAFKKMVADGSPAEKASLFHDTAARAYRLT
                     "
     gene            11611..13386
                     /locus_tag="AC16QF_14805"
     CDS             11611..13386
                     /locus_tag="AC16QF_14805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR6449878.1"
                     /translation="MASATLCRVRFELRVKNWRSYIPILDVITHYRRSDFSRDLTSGL
                     VVGVITIPQAIAYAFLAGLPAQAGLYACLVPMVIYAILGSSRHLVVGPVAVAALMVAA
                     AVGEHAPLYNESYLGITAVLGLQAGIFLWLLRLWKMGGVVNLLSHPVIVGFVNAAALL
                     IIVSQLNAFTGIADEGSKNPIDRLGQLVSKLGELHTTTTIIGVCCVVGLWLVRRWGVS
                     VAELLVHPLGFNLGDRQLIARLGPLLVASVAVIVVWAGDLNGTAGVATIGLVPGGLPS
                     LTVPPFNWDMWVDLAPSSAMIAVVAYVESFSIGTMIATRQRTRINAHQELIALGAANI
                     GAAFTGAYPVAGSFSRSSVNYQTGARTPVSSLVCAAVIILTLLFFTPLFTYLPHAALA
                     AIVIVSVTSLLDFRSFRTHWRIHREDSVTQMVTLASVLLFGVEAGLLTGVTLSIAFFI
                     RQSSRPNVTLVGRMGDTEQFRSARRYPVKTFEHVAAVRVDENLYFGNANQVENKLLKV
                     VHRRPATKHLVLVCSAINMIDVSGLEMLYRINQNLTDMGITLHLSEVKGPVMEQLAAT
                     DFISALAGTVYFTTDQAMRDLAPRT"
     gene            13398..14291
                     /locus_tag="AC16QF_14810"
     CDS             13398..14291
                     /locus_tag="AC16QF_14810"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR6449879.1"
                     /translation="MTDELDTADWECVLEGPRGAMTEAGFVLTAVAIEHRIELCGRIS
                     RLLVPPSQLAQAMAELESYQQENRPLPGSVYSVPTVDGGWAGVLGYLMVIWLQPSLEA
                     NQAFGWDWREAGHLQAGLVAAGEWWRCITALTLHGDLGHIVANSLFGSLFGLYVGRYL
                     GSGFGWLLILASGALGNAMNAWIQPDAFSSIGASTASFGALGVSAAFVWRRGYYRNRD
                     WRRSLAPIFAGIALLAFTGTGGENTDVVAHFTGFAAGALLGAAAAAFDIRRLGVSGQY
                     LAGAAAITLVVTAWSFAGTVG"
     gene            complement(14292..14870)
                     /locus_tag="AC16QF_14815"
     CDS             complement(14292..14870)
                     /locus_tag="AC16QF_14815"
                     /inference="COORDINATES: protein motif:HMM:NF016270.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HIR6449880.1"
                     /translation="MSHITTTFLRGLGVVLPLSLTIWFVVWLAVTTESLLRSVFLVIL
                     PEGLYLPGLGLIFGIVMVYGVGVLAQIFFLQSLWRHARNLLDRIPLVKTVYTAISDFY
                     QFFSSGPADGAARVVSVDVSDDVSVIGFVTGPSPSFMDQGGSRRIAVYFPMSYQIGGY
                     TLMVPEDRVSPLDIGVEDAMRVILTASVRSRR"
     gene            15007..16047
                     /locus_tag="AC16QF_14820"
     CDS             15007..16047
                     /locus_tag="AC16QF_14820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6449881.1"
                     /translation="MKLGLALGYSGAYLNIPLKKVQLAERLGYDSVWTAEAYGSDAIT
                     PLAYIAAHTERIRLGTGVIQLAARTPANAAMAFATLDQLAGGNRAICGIGVSGPQIVE
                     GWYGQPWGRPYYRIKDYVAIMKKIFAREEPVTHAGKEISLPYQGEGALGIGKPLKSIL
                     HMNPNIPIFLGTGMESTVRLTAEVADGWLPLGFVPETAALYDGWIDEGLAKGGKTSEQ
                     FERQAGTSVQVTDDVKSALDRLKPGIALYVGGMGHKTKNFHKEMMIRRGFGEAAERIQ
                     ELYLAKRKDEAIAAVPDEFVDQGALVGPKERIARRFRDWEDSGLTGLTISGDEEAVRF
                     MAECARLNFAAE"
     gene            complement(16059..17393)
                     /locus_tag="AC16QF_14825"
     CDS             complement(16059..17393)
                     /locus_tag="AC16QF_14825"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR6449882.1"
                     /translation="MSRHWCQGALASMLGCALLWSSCAAADQAGGWYAGVGIGWNHAE
                     NMELDELGGVVDYDFGAPVWSAALGWAFGPDWRVEVEASRQRNDVELVSFSAPVSASA
                     AALDPAPENAFSATTLAINAVREFAVGRDFRPYLGAGVGLAWTELDITDGSIEAQIDP
                     EPRDPLVDDQATSFAFQLIGGISLPLTRRMDLGVEYRYWQAPSVKLKEFTGTKLDTKH
                     AVHSGWLQLRYRSGGVERSRPAASPRPSPRGFYLTTFAGAGFSVDVEIDPGQTNFDAL
                     DVGPVAAFGVGYALSRRWRFELETAFRKNEVELLDFGLRFGSKQGSGRIRSTSLLASG
                     IYQFFPEARTRPYLGVGAGLVWADYDVDVLDELFVDDKDQSYAAEFRLGAEVSVSQRL
                     TVSAEYRYWISSDLKMTGPDGGPVEAYQQTHSMMVGLRYALGSGRSAGASGR"
     gene            complement(17434..18273)
                     /locus_tag="AC16QF_14830"
     CDS             complement(17434..18273)
                     /locus_tag="AC16QF_14830"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6449883.1"
                     /translation="MAYDYELLDVDIDGRVATVTVSNPPINLITVALYRELASLGAEL
                     RADPNLTVVVMKSADPDFFLAHFDVETILSFPVDGEAQRSAELSEYHLMCERFRTMDK
                     VTIAQIEGRVGGGGSELAASFDMRFGVRGRTKINQMEVPLGILPGGTGTQRLPRLVGR
                     GRALEIVLGGADLDAETAESWGYLNRIFEPEEIGGFVAALARRIASYPVEAVRLAKEA
                     VLGAETPVHQGLSNEAYLFQRLLRTEGAQRNMRRFLQIGGQTREGELRMGELCAELGE
                     ESR"
     gene            18375..19556
                     /locus_tag="AC16QF_14835"
     CDS             18375..19556
                     /locus_tag="AC16QF_14835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR6449884.1"
                     /translation="MDGRQQAIVNRIDELADELLAVSHAIHANPELAFEEHDACALLA
                     STARRFDLPVETGVFTLETAFQGSFGGRTSGPTVAILAEYDALPGIGHACGHNLIATS
                     ALGAALGLKAVEDELPGTVRLIGTPAEERGGGKELMARAGAFDGVDAAMMIHPAGVNL
                     ATMPCICVAEVEVIYHGRSAHASAMPHKGINALDGLLLAYQAISNLRQHIRDHERIHG
                     IVTEGGLAPNIVPDRAAGAFYVRAANEQDLARLKPRVQACFEAGAKGSGCELEVNWAN
                     VDYLDLNTNWPLANRFQAHGERLGREFLKPASLGGAGSTDMGNVSYRVPSIHPMLACA
                     PPNVVIHNPEFAKWARSEQGDAAAIDGAKALALTAADYLLDGSLQAEVNKAFAISKGL
                     A"
     gene            19573..20112
                     /locus_tag="AC16QF_14840"
     CDS             19573..20112
                     /locus_tag="AC16QF_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HIR6449885.1"
                     /translation="MNVWLFGYGSLIWRPEIAYVERRPARLHGWARRFWQGSHDHRGM
                     PRQPGRVVTLIEEEGGHCDGMAYLVDRGDVEAIFAALDHREKNGYRRFDVELHFSRYN
                     PAPDTTGVVYIAPPGNHAFLGPAPLAEMARQISECAGPSGRNVDYLLDLAEALRQLDA
                     QDAHVFELEAAVRHVSGSR"
     gene            20112..21311
                     /locus_tag="AC16QF_14845"
     CDS             20112..21311
                     /locus_tag="AC16QF_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449886.1"
                     /translation="MDISFSAEHERFRDEVRVFLDGALDDELREAQRFCPGIFLDYEH
                     NIRWHRILHAKGWVAPAWPREYGGTGWDLTRRYIWSTETTLAGAPSLAPMGLGMCGPM
                     LIGYGTEAQKAYYLPRILSGEDYWCQGYSEPGSGSDLASLKLRADGDGDHYVLNGTKI
                     WTTHAHYANRMFCLVRTDDGGRPQQGITFLLLDMDTPGISVEPIVFASGTHEVNQVFF
                     DDVRVPKANRVGEENDGWTVAKYLLEFERGGGGSAAYRTALTRVRKLAGQVTADGASL
                     AEDPDFKRALDETDVKLQAIQYTEFRIMAALSRGQNPGPESSIMKNLGADIGQRLTEL
                     TVEAMGQYIAPHQPEARQVGSNVPVVGPADGATAMPRYFNLRASSIAGGTNEVQKNIV
                     AKLVLGL"
     gene            21348..22085
                     /locus_tag="AC16QF_14850"
     CDS             21348..22085
                     /locus_tag="AC16QF_14850"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6449887.1"
                     /translation="MSELVLREDREGLAILTLNRPEALNALSPSLFVELRRHVDDIAQ
                     DVDNVGCVLLRGEGRSFSAGNDLKAIQAGERAPGPHFQAETLDAIEGLPQPVIAAVQG
                     HCYTGSLELALACDLIITARGAKIADTHGKWAMSPTWGMSQRLPRRVGLARAKELMYS
                     GRVIDGEEAAAIGLAVRCVPDEELLEAAVAMARTFLENSWFTLRADKMLVNGGLNHTL
                     ADGLAFERSNSPGRGPDLEERLERFGK"
     gene            complement(22105..22695)
                     /locus_tag="AC16QF_14855"
     CDS             complement(22105..22695)
                     /locus_tag="AC16QF_14855"
                     /inference="COORDINATES: protein motif:HMM:NF009205.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIR6449888.1"
                     /translation="MTRLDGVEEAAEVAKQFQFLENTLLKNRTLTLFGEINKDVAQRT
                     AEKLLALAYESDDPIVLYIGSPGGHVESGDTIFDMIRYIKPNVKAVGTGWVGSIATHI
                     YLAPDREDRFCLPNTRFLIHQPAGGFGGSASDVEIHAREIVRTRQRINAIIAEQTGQP
                     LERVEEDTDRDYWMSAEESVEYGIVGRIISSIDELG"
     gene            22756..23694
                     /locus_tag="AC16QF_14860"
     CDS             22756..23694
                     /locus_tag="AC16QF_14860"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6449889.1"
                     /translation="MPLAPEYQSFLEQLAQVPGPPLHELSPADARAAYRLLRPRDEQI
                     TVAAVNELAAPGPAGSIALRVYRPQGDGPFPMLVNFHGGGWVFGDLDTADTVCRNLCR
                     NTGCLVVSVDYRLAPEHPYPAAIDDAYAATCWLAEHASELEGNGRLAVAGESAGGNLA
                     AAVSLRAREEGGPAIDYQLLFYPVIDADFNRASYEENRSGYLLETATMIWFWDHYVPD
                     RSRRSQAYASPIRADDLGRLPPALILTAEFDPLRDEGNAFAEALKAAGTPAELICCAG
                     LIHDFCGTAHLFESSRKPFEDACAALRRAMSGTPES"
     gene            23739..24026
                     /locus_tag="AC16QF_14865"
     CDS             23739..24026
                     /locus_tag="AC16QF_14865"
                     /inference="COORDINATES: protein motif:HMM:NF015735.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR6449890.1"
                     /translation="MLYVMICEDKPDSEALRLANRADHLEWVGGKTAMIRLAGPMLSD
                     DGEHMRGSLFIIQAESADAVRQFNGEDPYTRAGLWGKVTIRPFRQVLPADS"
     gene            24040..24756
                     /locus_tag="AC16QF_14870"
     CDS             24040..24756
                     /locus_tag="AC16QF_14870"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6449891.1"
                     /translation="MRKAVILGVGPDLGLGAQLCKRFAGEGLHVMVAGRTDTSVEAVA
                     GDIRANGGAATAVVADATSEDDVRALFDQAGDDLDLAIYNAGNNMPGRIIDMEADYFE
                     NSWRVGCFGGFLFGREAVRRMVPKGAGTLLFTGASASLRGRAKFGAFNSAKGALRNLA
                     QAMAKEYGADGIHVGHVIIDGPIAGDKIIKAFPEYAQRLGPEGMIGIEGIVEGFAFLY
                     RQPRNAWTFEVDVRTSLEKW"
     gene            complement(24827..25234)
                     /locus_tag="AC16QF_14875"
     CDS             complement(24827..25234)
                     /locus_tag="AC16QF_14875"
                     /inference="COORDINATES: protein motif:HMM:NF024844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR6449892.1"
                     /translation="MALNASDLNAGDTYTECLVENLSRTQIVMYAGTSGDYNPLHSDE
                     VFTTQIAGYPSVFAHGMLTMGMTGRMLTNYVGDGRLTKYGVRFTNQVWPGDTLNATAT
                     VDEVADGIVKLNVETTNQDGAVVLSGYAEARVD"
     gene            complement(25246..25740)
                     /locus_tag="AC16QF_14880"
     CDS             complement(25246..25740)
                     /locus_tag="AC16QF_14880"
                     /inference="COORDINATES: protein motif:HMM:NF024844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAS1-like dehydratase domain-containing protein"
                     /protein_id="HIR6449893.1"
                     /translation="MSVDRFPIEASHIMMFARSIADDNQIYYDEEYAKGTEPGTIIAP
                     PTFAQASAQFDPDYFLRPKIGEPWFGSAKEATGVKREGGGSGGGGGGGLHAEQHFEYH
                     RHLKPGDVLSAEVKPGKTWEKASKRAGKLLFSETITEYRDQNGELVITARGVGVRTER
                     PVDR"
     gene            complement(25849..26628)
                     /locus_tag="AC16QF_14885"
     CDS             complement(25849..26628)
                     /locus_tag="AC16QF_14885"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6449894.1"
                     /translation="MSEFENILYEVQGPMGLITINRADKHNAISLATLDELHQAVGLA
                     AADDAVRVLTITGAGDKAFASGSDLSEVLHRDFRKALEPIVQGLADQLERMPKPTIAA
                     INGICMGGGLEVALGCDLRIAVPKARFATPEGKLGIIPGGGATARLPRIVGRGWGMEM
                     LLMGEPVDAERALQIGLVTRVVEPAELIPEARRMADHLAGFAPFVPRTMKAMVHFGME
                     ASLAGALMFEKYAQGALVQTDDKVEGITAFLEKRDPEFKGR"
     gene            complement(26670..27326)
                     /locus_tag="AC16QF_14890"
     CDS             complement(26670..27326)
                     /locus_tag="AC16QF_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HIR6449895.1"
                     /translation="MANWKTALVAGLVALGSAGCETAYYGAMEQVGVYKRDILVDRVE
                     DAVEAQQDAKEEFADALEQFASVVAVEPSRLKDTYEELSDAYDSAEDRAERVRDRIDS
                     VEDVSEDLFDEWSEEIELIGNPKLKSASASQLRASQRKYDELIKGMRRAERKMEPVLD
                     TFRDHVLFLKHNLNARAIASLKSELTSIEADVAGLIREMEASIAQSQAFIEEMQLVDG
                     "
     gene            27568..28377
                     /locus_tag="AC16QF_14895"
     CDS             27568..28377
                     /locus_tag="AC16QF_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6449896.1"
                     /translation="MTAYTDTAIELLMTYGPKFLLALVVLIIGLWIIKRVVKVTSKAM
                     ERGNAEPTLAKFVASLVSVGLKALLIISVASMIGIETTSFIAILGAAGLAVGLALQGT
                     LANFAGGALILLFRPYKVGDWIDAQGVAGTVHAIQIFNTILKTPDNRTIIIPNGAISN
                     GIITNNSTEPTRRVDFVFGIGYGDDIAKAKGIIERLIGADERTLSDPAAQIVVSALAD
                     SSVNLTVRVWVNAADYWGVFFDLTEKVKLAFDEEGVSIPFPQQDVHMHQAG"
     gene            complement(28620..29900)
                     /locus_tag="AC16QF_14900"
     CDS             complement(28620..29900)
                     /locus_tag="AC16QF_14900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6449897.1"
                     /translation="MPEGRRGLWGPASLLARTNLILAVGSLVIALIAIAALNVFVIEP
                     IAERSADDEAALLVLSAQTWVELPPAARPYFEIELAQNHDLIVSAEVRDLPFADLDQP
                     YLSLVEEKLSQRLNTPARLREGDDLIWVTVPMGGYDIQIGFASNRRDIQPLYVGIIIV
                     GLGAAVVFITSLLIVLRIARPLVETAERAETYRGGENFEPLPERGPRELVSLTRNFNT
                     MAREITALLANRTTLLAGISHDLRTPLTRMRLALELLPDNVDPKLVERFERNLESMDE
                     LITDALRFARGTSEVAQEVELKPFVEDVVASFDQSIPLTSDGDDVRVSVAPGALQRVL
                     INLITNALQHGKGVRVHIAGREVHVLDSGPGIPEEYRERVFQPFFRLDDSRSVATGGS
                     GLGLAIVQQLCQAQGWRVAIQEADNGEIDVFVTL"
     gene            complement(29893..30603)
                     /locus_tag="AC16QF_14905"
     CDS             complement(29893..30603)
                     /locus_tag="AC16QF_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6449898.1"
                     /translation="MDAEGAKLLVVDDDPEIRELAQAYLSQQGFEVDCVDSGEAMDAY
                     LESNEVDLIILDLMLPGEHGLSIARRLKTDGDVPIIIVSAQGEEIDRIVGLEVGADDY
                     IGKPFNPRELLARVRAVLRRAQRTGQASSAENRVCFGSFELDLSAHQLTADGKTVPLT
                     SGEFDLLAILVSHPNKVLDRDRILDLLTGAERSPFDRSIDVRVTRLRAKIEPDPSTPV
                     FIKTIWGKGYMFCPDGRA"
     gene            complement(30613..31035)
                     /locus_tag="AC16QF_14910"
     CDS             complement(30613..31035)
                     /locus_tag="AC16QF_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449899.1"
                     /translation="MLFTSLSGGAFAADEAVRAGDAASIPNVYAAKSDQELTALAAQW
                     ESLDTAERRALLTEVKMRMARQRGPDGTIRITFQRRYGRIIRNPDGSVVRIETNVVRV
                     RPGAAGNQTYGVGFEQRTADGQREEQSPPVITVKDPSP"
     gene            complement(31299..32483)
                     /locus_tag="AC16QF_14915"
     CDS             complement(31299..32483)
                     /locus_tag="AC16QF_14915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6449900.1"
                     /translation="MKDVVIVDSVRTGLAKSFRGSFNLTRSDDMLAHCIDALLDRNGG
                     VSPHEVEDVVVGAASHVGEQDGNLARLAVVLSKLPITTAGLTINRFCSSGLQSIAIAA
                     NQIATGQTQVAIAGGVDSISMRTRQEAAKAELNKRLVEEKPDIYMAMGNTAEVVARRY
                     QVTREAQDEYALQSQQRYAAAAEAGYVAEEIVPMAATMLKVDKETGEESRVDVVVDRD
                     ECNRPTTTLEGLASLPPAFEEDGSVTAGNASQLSDGASMTLLMSAERAAQLGLEPLGI
                     YRGFTVAGCEPDEMGIGPVFAIPRLLENAGLKQDDVDLWELNEAFASQCLYCRDALGI
                     DNEIYNVNGGSISIGHPFGMTGSRMTGLVLRELKRRGKRYGVVTMCIGGGQGAAGLFE
                     AA"
     gene            complement(32772..33116)
                     /locus_tag="AC16QF_14920"
     CDS             complement(32772..33116)
                     /locus_tag="AC16QF_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449901.1"
                     /translation="MSTIKNWMMALLGVVVLVGFVGCGQDAGSGPADAPAAAEEAAAD
                     ETAGGFIQPAAEAAEEAMDEGMEALEEAADETATEAAAEAAEMMEEAAAEAEEAAESA
                     MGEMNITPPGSN"
     gene            33295..34137
                     /locus_tag="AC16QF_14925"
     CDS             33295..34137
                     /locus_tag="AC16QF_14925"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR6449902.1"
                     /translation="METITLVEGLCFGEGPRWRDGRLWLSDMHAQQVIAVDGDGHVQV
                     VVEVPNLPSGLGWLPDGDLLIVSMRDRRVLRHDGARLHTHADLSQLASFDCNDMVVDV
                     HGRAYVGNFGFDLHGGEAPRAAELICVEPDGTARIVADDLLFPNGTVITPDGNTLIVA
                     ESWGGRLTAFDIDQAGNLSNRRDWAKLPDGAVPDGICLDSDGGIWTASPATNECLRQI
                     EGGTVTHRVPVNQGAFACMLGGDDGRSLFILTAGSSAPDACRAARSGRVELVTAPFPH
                     AGWP"
     gene            34176..>35050
                     /locus_tag="AC16QF_14930"
     CDS             34176..>35050
                     /locus_tag="AC16QF_14930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449903.1"
                     /translation="MYENYESPWLNDELRLLQDAAAKFFQQEFVPLNEKWINQGKVDR
                     EAWYKAGEAGLLCAEMPVEYGGGGGDYRHETVIMEEQLATGVTGFGNQVHSSIVAPYI
                     LHYGSEDQKQRWLPKMATGELVGAIAMTEPNTGSDLQAVRTSAIRDGDEYVINGSKTY
                     ISNGQHADLVIVVAKTDPAQGAKGISLIVVETGKSPGFERGRNLKKLGQKAADTSELF
                     FADCRVPVTNRLGEEEGRGFVQLMQQLPQERLNIGQAAVVAMERAIGITLDFVRERRA
                     FGQRILDFQNTRFKLS"
BASE COUNT         6350 a        11688 c        10904 g         6108 t
ORIGIN      
        1 cgtcccagca cgcgcggccc gctgcctggt accgacgacg gctgaacaga cactgggcga
       61 acgtgggcgg cgggctctat ctcaagcgcc ccctaggcgt aaccgtctgg accctcgatg
      121 cgctgaacac cgtcccctcc tagagacgtc gaaggttgtc gtcgggattg cggtcgatca
      181 tcttgttgta aggatcgatg cctacccgct cggggcgctc gtccacgcgt accgtgaacg
      241 tctgctctcc cgataccacc tggcgcctct cggcgaggag cggatccggc agatcatcct
      301 cgctcagatc ttcgggcgct tccggaaaga cggccagatc gaacatctgg ctgagcggca
      361 cctccgcctc cctgccctcg ccgtccgcgt cgagcttgcg ggccgcaacc gtgatcgtca
      421 catcgaaacc cccgtcgttc tcagccaccg atgcgtccgt caccgcgagg tcgtataccg
      481 tgatcctctc gaacagatcc gtgatgagcg gctggtgctc cgccccggcc acggcccgga
      541 actcgtccag cagatccctg gaggttggaa acaagccttc cttgaacccg tatttcgcga
      601 tgaagctctt gagcgcggcg ttgaccttgt cctcgccgat ggcatccttg agcgcataca
      661 tcaccacgct gcccttgctg taatggatgt acccctggtt ctccacgtaa agcagaggca
      721 gctcctcgat cagctcaccg ccgcgggact gcagatagtt gtccagctcg tacttgagga
      781 aacggcgcat gcgctcgggc ccatactcct gctccatcac catcagcgcc gagtactgag
      841 ccagagtctc gacgatgacg gtcatcccct gcatctgcgc cccgaccacc tgatgcgccc
      901 accactggtg ggcaagctcg tgcgcggtca cgtagaacac gtaatcgatt tccgcgggat
      961 cgcgaagatc ggcgatgaac ccgatgccct cggagtaggg aatcgtgttc gggaacgact
     1021 gtgcgaacga cgcgtacgcg ggaaactcca ggatccggaa ctggcggtac tggtaggggg
     1081 agaattcccg ggtgaagtag tccagcgact tcttcgtggc ggtgatcatg gtctcgacgt
     1141 tgtacgggtg cttggggtcg tggtacacct ctaagtccac gtcgtcccac ttgtcgcggg
     1201 ccacgacgta tcgcgcggac gagacggaca cgaagggcca gatcggctga tccatcgcgt
     1261 actcgaagaa gcgtcgatca tcttccaccc actctcgctg gagatagccc ggggccacgg
     1321 caatctgatc ggccgaggtg ctgaccacgg cgcgaaagtc gctgcgaacg ctgacgccga
     1381 gctggttggt ccccagccag tccggatcgc ccagcttggg aaggcgctcc acgggcggca
     1441 gcccatactc gcgacggacg ttgttgtcgt ttagttcccg gccgctgtcg tagccgagcg
     1501 tcggggctat ctcggtgctg tcgacgaaag tgccgttgcc aaccacccga gtcgtagcgc
     1561 ccgaattcgg aaaaccctcg ttgatccagg acaggtccca ggaaagcgtc gtttccgctc
     1621 ccggcggcaa cgcttcatcg aggacgtact ggtagtagcc gagccgctcg tcttcggcga
     1681 cgagcccggc tcgctctagg gacagctcgt tgacccggag gagcggcggc agcgtaaggt
     1741 gaacgtcgct gaccggctgc tcgttggcgt tgatcagacg cgcggtgccc ctcgactcca
     1801 accggcgctc atcgggaaac acgtcgaccg ctaggttcag atccacgacc tgcaaaggcg
     1861 gcagccgacg gtgctgtcgg taagcctttt cgtaatccgc ctgggcttcc tccaggtcct
     1921 cggaggtcag gtactcgttc aagacgttgg tgttgtagaa gatccaaccc cccaggccgc
     1981 agaaggtgag ggcggcgagg accaccacgg cgccgaccgc gcccgagaaa cgctgacggg
     2041 ccgtcgccag cctctctgcc gccgtctgca ggccgcgggg atagaaaaga tgggcgacga
     2101 cgacgaggat gacgcacaag ctcgcccagt acgcaaacgc cgaaacgatt cgcccgacga
     2161 aatgcccgta gccgttcatg tccgaataag gtgcggcggg cagcccgaag ttgtagagca
     2221 tgtgttcgaa gccgaaaccc tccagggttg tcaggcccag aaagatcacc agcagcgccg
     2281 ccatgccgag aaacttgttc gacgccagcg actggatgag caccgccggg accgcccaca
     2341 gatagaacga accgccgatc acgccgaaca cgctgacgag atagagcaac ggctcgaact
     2401 cccggtagcc gttgagcgcc tggacgagca tgccggcaag cacgaccacc aggtacagca
     2461 ggctgatcac caaccagaga ccgaacacct tggagaggat catgatgccg ttgggatacg
     2521 gcgcggcgtc gaggtagtcg gccaccccgc tctggcgctc gcgatgcacc agctcgccgg
     2581 aatagtagat gaggatcagc agcaccacga acgcaaaggt gctccccacc acctgagtca
     2641 tgatgcgggt gacggggtaa acctccgttc cgtaccactg gctgacggct ccgtagaaac
     2701 caccgatcac gttcagcatc ccgaaggcca gcagcacgta aaagggaaca ctccggatga
     2761 tcccccggaa gtccatgcgc agctgcgaga ggaggctcgc agtcatcccg gcacttgccc
     2821 gggcaacgtc aaccacgtga cggggcgcgg ctcgcgcctc ggaccggttc ggccggcgtc
     2881 gccaccaccg cttgcgggca ggcttcagct cgaaggcaaa tcgggccacc gtgatcccca
     2941 ggacggccgt cgcgacgagg acccagatca cgcggttgag cagcagcgtc cccgcgagct
     3001 ccggaacccg gaagttccga tcgaacactg tccagtatcg ggtgatttct cccagcgcca
     3061 cctgtccgaa cggttctccg agcgccagat accggatgtt ctcaggatcg gtggcgctgc
     3121 cgatcaccgc gaaagagacc agaaacccga gcgcggcgac gtaggacgcc atcatcgatc
     3181 gggtcagagc cgccacgctg aagaacaaag cgcagatgat gaacatgttc ggcagcacga
     3241 tgaccagcat gctgaacaca taaggcgcca ggctgaagct gccgatacgc tccgcatcga
     3301 gccagggcat gaaggtgccc agcatggtcc cgacgacgcc ggcggtcacc gcggcggtgg
     3361 cgaagacgca ccccccggca aatcgtccca gcagatagct cctctcggat atcccgctgc
     3421 tgtagaagat ctcggccgtc ttggtttcgc gatcgcgcgt gatggcgctg gccacgaagg
     3481 ctattgccgc aaacatgccg atgatggtga gcaccgtgat cgtctggatg atggcaaacg
     3541 atgcgttcag gttcaggttg ctgccgacac cgccgacgct caccgactcg ctgccggtga
     3601 tcagaaaacc aaaaaggaac cacaccgtgg atatcgtcag gaacagcggg gagcggacct
     3661 gaaagcggct ttcgaatctg aagacctcga gaaacatggc gcctcctctc agtcgaccac
     3721 cgggttcatg ccgtgcttca tgagggtggc gaagtaaacg tcctccaggc tcggctcgac
     3781 cgcggcgagg ccaccctcgg ggtcctgctc cgagagcacc cttagttcca cctgccccgc
     3841 caccagtcgc gtcgacacca ccggcaggga acgccgcaac ccttcggcct cgtcgcgggg
     3901 aaccactttc gaccacagcc gtcccgcaag acgcccggtc agctccttgg gttcaccctg
     3961 caccagaatc cgcccgccgc ccatgacggc catgttcggg caaagctcgg caacgtcctc
     4021 gacgatgtgg gtggagagaa tgaccaccac ctcctcgccg attctggcca gcaggttatg
     4081 aaagcgccgg cgctctgcgg gatcgagccc cgccgtgggc tcgtcgacga tcaccagctg
     4141 cggatcgccg atgagcgcct gggcgatccc gaagcgttgg cgcatacccc cggaaaagct
     4201 gtcgatgctc ttcttgcgca cgtcccagag gttcgtctgc accagcagct gctcgacgag
     4261 cgctttgcga gcgccgcgac cgccaatgcc cttgagcgct gcgatgtgat cgagcatcac
     4321 ttccggagac gtgcgcggat acgcgccgaa ctcctgcggg aggtagccca gcacgcgacg
     4381 cagggcgttg ggatccgcga gcacatcgac gtcgttgaag tgaatggaac cgctgtccgg
     4441 gctctgcagc gtcgcgatgg tgcgcatgag cgtggacttg ccggcaccgt tcggcccgag
     4501 caacccgaac atgcccttgg cgatctccag atccacgccg tccagcgcct gcacaccgtt
     4561 gggataggtc ttcgacaaag cgtttatctt cagcatgctt tctccttgca gagctcgatc
     4621 ggtcgttcct cgacgcccga gaaccaccgc gctcccggcg cggcccgttc caccaacagt
     4681 tcgaccatgc cggccaaccg caatgcgtgc gcgccaccac cttgccggcg cgcgttggcg
     4741 aatcgagaat acctcaatga gcgaactggc gcggcaagcg gctcttgata ctgccgggat
     4801 gattcgacgg ggagacgcat ccgccgtgga agtcatggac gcacactacg atcgcatcga
     4861 aatcctgaac ccccagatca atgcgctcgt caacgttctg cccagagagg cggcactcga
     4921 gctggctcgc caagcggatc gggcgcgcag ccgcggtgag ccgtgcggtc ctctgcacgg
     4981 tctgcccatg gccgtcaagg acacccatga cgccgcaggc tttccgacca cctggggatt
     5041 tgtcccctgg gccgaacgcg tggctaggca cgatggagcg gtggccgcac gccaacgggc
     5101 cgcaggcgca cttttcatcg cgaaatccaa cgtgcccgag ttcgccctgg gatcacacac
     5161 gttcaataac ctcttcggaa ccacgagcaa tccctacgac ctgtcgcgca cggccggagg
     5221 cagcagtggc ggcgccgccg cggcgctggc aacggggatg cttcccctgg ccgacgggtc
     5281 ggacatgggc ggatcgctgc gcaacccggc cgggtactgc aacgttgtcg gccttaggcc
     5341 ctcattgggg cgcgtcccgg cggatggccc gttcggctgg tttgctcggc tgctcacccc
     5401 cggccccatg gctcgtaacg tgcccgacgc ggctctgctg ctgtccgttc aggcaggccc
     5461 caatcaggac gaccccctga gccggccgga gccgggccga cacttccgcg atctgcccca
     5521 cgccgatccg gcaaccctga agatcgcctg gtccgcagat ctcggctttg cacccgtgga
     5581 cgccgccatg gtccggctgc tcgaggcagc ggcggtcgtt ttcgaagacc tcggctgcga
     5641 ggtggagaat gcctgtccgg acctgcgcgg aggcatgtcg gtattccaga cccagcgcgc
     5701 ggccagcctg gcccagaccg gccgggccct ggatcgctcg ctaccgcgct ggcgcgaaca
     5761 tgccaaggaa accatgatct ggaacgtgga gcagggctac gcgctcaccg cagaggatct
     5821 gttgcgctgc gaggtcgaac gtacggcgat ttaccagcgc gtcggcgctt tctttgccca
     5881 ccacgacgcg ctgctgctgc cttctgccca ggtaccaccg ttcgacaagt cgctggattg
     5941 ggtgcgcgag atcgatggcc gggccatgga gacgtacatc gactggatga ccatctgctg
     6001 cgccgtgacc gtcaccgatc tccccgcgat ctcagtcccc ggcgggttca cggacgacgg
     6061 cctgcccctg ggcctgcaga tcgtcgggcg acctcacggc gacctcgatc tgctggccat
     6121 cgcccggctg ttcgaggagg ctaccggcca tggccagcgg cgtcccccgc agagcgagca
     6181 tgccggctga ataacgaccc tagagcgcgg gaaacaggct cggcttgaag aggacgatct
     6241 cctcgtcgac cacgagcgtg tccagaacct cccggacggg aaatttgcgg tagatgggga
     6301 cggtgccggc ttcctgagct ttccggcgag cctccacgag cacgtagtcc tgcaacgagg
     6361 cgatgtccac cacggtgtac acgtcctcat cggcgcaaaa atcgcgaccg acgagacagc
     6421 gaaagtcgct tctcaaaacc aacgaacaac ggcccgaagc gggtccaacc cccaacaccc
     6481 acagtctatc cacgccgtaa atggttgatg taaaaaggat gtaagagatg taacggagac
     6541 ctgacgccat gccggaatcg ttacgagtca tggtcgtctg cgcccatccc gacgatgcgg
     6601 agtttcacgc cggcgggctg ctcgtccagc tggccgcagc gggcacggac atccacctgc
     6661 tcaccctcac ggacggctcc gccggtcacg ccgagctgga tcgcgcctcg ctcgcccgac
     6721 gacgggccag ggaagcccag gccgccgctc gccggctcgg cgcgcggtgc accgtctggg
     6781 atacgccaga cggcgagctc gagccgagcc tccagctgcg cgggcgactg atcgtcgccg
     6841 tgcgcgaatt cgctccccac gtgctgatca cgcaccgctc gggcgattac cacccggacc
     6901 accgagcagc agcccagctg gtccaggacg ccagcttcct gctgcgcgta ccgaacgtcc
     6961 agcccgatgc gcccatcatg aacgaggatc cggtaatcct gggattctgc gacttcttcc
     7021 gtcgcccctc gcccttccgg tcagaccttc tggtggacac ggaagagcag ctggaagccg
     7081 tggtggagct gctggcgtgc cacgaatccc aggtttacga gtggttgcct cacatcatgg
     7141 gcctcgaggt gggggagaac cgtcgcagct ggctgagaga gttctacaca cagcggccgg
     7201 cggccgtcgc ccatgcccat gccgacgacg gtgcccgcta cgccgaggct ttcgagctgt
     7261 cagagtacgg gcgacaggtc acgccacaag agctggccgg gctgctcgcc gcgggctctg
     7321 ccaaagccaa ccacagcggc tgacttcaac ccgccgaccg ctcggcaggg gggcagcagc
     7381 cctgggcttc gggctcggcc ggcgcgcagc acgccagggc ctcgggctcc gcgaagaaag
     7441 tcggggagtc ctccaccacc cgataccact cccacatcat gccctgcgga tcgtagctga
     7501 cgactttgtt ctgctttgcg aagcagcaga cctcttcggt ctgcacctcg tgctccacgc
     7561 gcgcatcctc gagccgtccg gccaccgcct cgatgtcctc gtcggcaaag gcctcgaagc
     7621 ccaggtgatt cagccgctcg tcggctccgg gcgcttcgaa gagcacgagc ttgagcgccg
     7681 ggtcttcaat cacgaagttg gcatagcccg gctcccgctt gttggccgcc acgcccagga
     7741 tccgcgagta gtagctcacc gcctcgtcta tgtctgcgac gttcagtgcc agttgaagac
     7801 gcatgcgatt ctcctgcatc aatactttga aaatatattt ccactttatt cgaaataatt
     7861 gacccgcgcc aataccgttt cgttatttct agcctcatgg aaataacgta aactgcgatg
     7921 gcccgaagga ttcaagcaga ctccagatga aactagatgc cgcctcggca tgcctcgaaa
     7981 gcctcggcaa cagcacccgg ctcgccatct acagagccct cgtgcgtgcc ggaccggccg
     8041 gcttgcccgt cggaaagctt caggctcgtc tggacattcc cgggtccacc ctttcgcatc
     8101 acatcgctca cctggtgaaa aacgatctgg tctcccagac ccgcgagggc cgcgtgcttc
     8161 gctgcacggc gaactacagg accatgaacg atctgatcac ctacctgtcc gacgagtgct
     8221 gtcgggacac cccgaggacc acctgatggc tcgccggctt gcaggcgagt tcatgggcac
     8281 cctgctgctg ctggccaccg tcgtgggatc ggggatcatg ggagagcagc tttccggggg
     8341 caacgcggcc atcgccctgc tcggcaacac catcgccacc ggcgccatcc tggtggtcat
     8401 cattttgatg ttcgcgcccg tctccggagc ccacttcaac cccgcggtca cgctcacgtt
     8461 cctcctgcgg cgcgagatcc ccgcgggtgc ggcggccgcc tactgcgtga cccaggtggc
     8521 cgctgggctg gccggcgtgt ggctggccca tgggatgttc gggctggaaa tcgttcagac
     8581 atccgagaat tcccggtcag gcttcgggca atggctggcc gagggggtcg ccaccttcgg
     8641 cctggtagcg accatactcg ccgtgctgcg ctcccgtccc gacctggtag cgttcgccgt
     8701 ggggctctac atctccgccg gctactggtt caccgcgtcg acgtccttcg ccaaccccgc
     8761 agtgaccatc gcccgggcgt tcacggacac gttttcggga attcagcccg accacgtccc
     8821 cgcattcatc gcagcccagc tcgtcggagc cgcgctcgca acctggacgt tcggctggct
     8881 gctctccgag ggcgaaagga ccaactaacc gtcccgctga ggcttcgagc ttgctgcggc
     8941 cacccgcgag cgaaagtcct cggtgctgtt cacccaggga tatggctcgt cgggcaccgg
     9001 cagatcaaga aaatcgcaca acggtcccca accggcactc gcctcgaaga cgaggagctt
     9061 ttccggcggc agcgcatcga tgacgtgctg gccgtgggct tcgtagatcg cgatgacgtg
     9121 ctctttgtca tccatgcgcc cgtcgaacag accatcccag atcacctcga acaccatgtc
     9181 catgtgctcg cggatcgccg gatccgcctg ctcgcgccgg gcaacgctcg acggatagat
     9241 cgtgttcatg acgctggcgt accagcgctc ggggtcccgg cgggtgagga tgaccttggc
     9301 gtcggcaaag tgctgaagct gcgcttccca gaagttgcag gagggccagt ccaccgaagc
     9361 ctggtagccg gcaaagaggc cgtcccagcc caccgactcg ccccgtgccg ccgcccgcca
     9421 gacggccgcg tgaccctcgt tctgcatgac ctcgagcatg tggtaacact tctccagccc
     9481 caggcgttcc agggcgagct tgagcgagag cgtgcccgtt cggccgaagc ccgcgccgat
     9541 gactttcagc gtcacgatga gttcctcgac cttgtcctca cggctggtcg gcgaccgtga
     9601 ccgtgatcaa ctcggacatc accgggggat cgtgaggcac gtgcagataa ttgcccagca
     9661 tgagctgcag gcggtaggtt ccggccggaa ggctgagctg tgtttccgtc tggccgccgc
     9721 cgaagtggcg aacctgatcc gtggcaggca gcggcacttc gggattcacc gcttcctcga
     9781 gattgatcag gaggtggtgg tggccggtgt tgggcgcgtc cacgccggcc ggggcgacgc
     9841 ccatgccttt caggccgaac ttcaccgtga acggcccact caccacggcg ccatcggcgg
     9901 gggctatgaa atagagcgac gccccttctg gtgccggcga gcggatgccg tccgccagca
     9961 cggccgccga cgtcaccaga gccagcgcca aggcaattga tctcaagccg ttcatcgtga
    10021 tcctccgtcg atgttttcag gtttgccgtg cccgcccgtc ggcctccggg atcaggcgga
    10081 acaacgggat gacccgtcct gcccgctgct cgtagcgtcg gtatcccggg tagatggcga
    10141 ccgccgactc ccaggccgcg tctcgatccg ccccgctgag ctcctccacc cggaaggtgc
    10201 cctcgcggcc ggcgagcagg cagctgaccc ggcgctgggc acggagattg tgacaccagg
    10261 ccggatcata cggctttccg ccgttggaac ccacgagcag cgccccgtcg ccctcgggaa
    10321 cgtagagcag cggcacctcc ctgagcaggc cgctgcgcgc ccccgtgcac cgcaacagca
    10381 acgtcgggat cacgaagctg aggcggatgc gtccggaagt tgcccgcatg aggggtctgt
    10441 cgacccaggt ggcgagattc atgaagaata cggtcaccgg cttgagcgac acgaagaggg
    10501 aaaatccccg ccagacggcg tcccgaatgg tcacgtcagg cgataggctc gagcggccgt
    10561 gtcgtgaaag agcgaagcct tctccgccgg agacccatcg gccaccatct tcttgaaggc
    10621 attccagagc acgtgatagg agcagctctg cttgtccacc gggaagttgc tttcgaacat
    10681 gcacctgtcc acgccgaagc agtcgatggc atgccggtag tagcggccgg tcgccgccac
    10741 gatctcatcg gaggtagcgg gccgttcacg cttgtgaaac ccgaagccgt tgacgggcat
    10801 gaccaggcca ccgagcttgg cgtagacgtt gtgacactcg gccagcgcag ccacgtcgcg
    10861 ctgccaggcg gcgaaaatct ggtcctgccg gccttcgtag gggccgatgc cgaggggccc
    10921 gccgaagtga tcgaagatga tcgtcgtgtg cgggaaagct cgcgccaggt cggtgagctg
    10981 ggggatctgt gggtggtaga gccaggcgtc gaagctcaat ccgcgcttgc cgagctccgc
    11041 aaaacccgcc cggaattcgt cgtcgagcaa caaaccctcg gacgggttgg tgtgggagtt
    11101 gcgaaccttc tcgctgggat cccagccggc ggcgtgacga atccccttga accggtcgct
    11161 cgccgccatg tgggcgtcga gcacggggcc cgcggaggat ccgagggtca ggtctacgta
    11221 gccgacgata ccggcacaca cccgggcggg cccgtaatcg ccgcttgctg acatggccgc
    11281 gacgccgttg acgaactccg tttcacccag ggatctcagc tcctcgggtc cgtcggctcg
    11341 atacatgctg gcacactcca cgaaaaccgt agctaccacg ttgtgcccgc tgcccgtgtc
    11401 ggcaagcagc tcgtcgagaa ggtaccggct cccaggatgg tcccacaggt gatggtgggg
    11461 atcgcagatg ggcagatccg gttcgaggac gtcctcgaca acctgattca gccagctcgc
    11521 ctggtcgcgg gccatgcggt ctctccggga atgattcgat ttgcgacgag gctatcgctt
    11581 cgcaggcgaa aagcaatacc acgcgaatgg atggcgtctg ctacgctttg ccgtgtgcgc
    11641 ttcgagctga gagtcaaaaa ctggcgcagc tacattccga ttctcgatgt gatcacccac
    11701 tatcgacgat cggatttcag ccgcgacctg acgtcaggac tggtggtcgg cgtcatcacg
    11761 atcccccagg ccatcgccta cgccttcctt gcgggcctcc ccgcacaggc aggcctgtac
    11821 gcgtgcctgg tgcccatggt gatctacgca attctcgggt cttcccgaca cctggtagtg
    11881 gggccggtcg cggtggcagc gctgatggtg gcggccgccg tgggcgagca cgcgccgctg
    11941 tacaacgagt cctacctcgg cattacggcc gtgctgggcc tgcaggccgg catattcctc
    12001 tggctgctga ggctttggaa gatgggcggc gtcgtcaatc tcctcagcca tcccgtgatc
    12061 gtcggcttcg tcaacgcggc cgcgctgctg atcatagtca gccagctcaa cgcgttcacc
    12121 ggcattgccg acgaaggcag caagaatccg atagatcgcc tcggtcagct cgtctcgaag
    12181 ctgggcgagc tgcacacaac gaccacgatc attggcgtgt gctgtgttgt cgggctctgg
    12241 ctggtacggc gctggggggt gtctgtcgcc gagctcctgg tgcacccgct gggcttcaac
    12301 ctgggcgacc ggcaactgat cgcgcggctg gggccactcc tggtggcctc ggtcgcggtg
    12361 atcgtcgtct gggcagggga tctgaacggc acggcaggcg tcgccaccat cggcctggtt
    12421 cccggcggtc tgccgtcact caccgtgccg cctttcaact gggacatgtg ggtagacctc
    12481 gccccgtcat ccgcgatgat cgcggtggtc gcctacgtcg aaagcttctc catcgggacc
    12541 atgatcgcga cgcggcagcg aacgcggatc aatgcgcacc aggagctcat cgcgctgggc
    12601 gccgccaaca tcggcgcggc tttcacgggc gcctatccgg tggccgggag cttctcccga
    12661 tcaagcgtca actaccagac cggagcgaga acgccagtca gctcgctggt ctgcgcggcg
    12721 gtgatcattc tcacgctgct gttcttcacc ccgctgttca cctacctgcc tcatgcggcg
    12781 ctcgccgcca tcgtcatcgt atccgtaacg agcctgctcg acttccgcag cttccgcaca
    12841 cactggcgga tacaccgtga ggacagcgtg acccagatgg tcaccctggc ctcggtgctg
    12901 cttttcggcg tggaagccgg gctcctcacg ggcgtcactc tttccatcgc gttcttcatc
    12961 cgtcagtcca gccgtcccaa cgtgacgctg gttggtcgga tgggggacac ggagcagttc
    13021 cgctccgcac gacgctaccc cgtcaaaacg ttcgagcacg tagcggccgt gcgcgttgac
    13081 gagaacctct acttcgggaa cgccaaccag gtggaaaaca agctgctgaa agtcgtgcat
    13141 cgaaggcccg ccaccaagca cctggtactg gtgtgcagcg ccatcaacat gatcgacgtc
    13201 agcggcctgg aaatgctcta ccgcatcaac cagaacctga ccgacatggg aatcacgctg
    13261 cacctttccg aggtgaaagg ccccgtgatg gagcagctgg ccgccaccga cttcatctcg
    13321 gcgctggcgg gcaccgtcta cttcaccacc gaccaggcca tgcgcgacct ggcgcccagg
    13381 acctgagcgc tagcggcgtg accgacgaac tcgacaccgc agactgggaa tgcgttctcg
    13441 aagggccccg gggagccatg acggaggcag gattcgttct gacagcggtg gccatcgagc
    13501 accgcatcga gctctgcgga cgcatttcca ggttactggt ccccccatcg cagctggccc
    13561 aggcgatggc ggagctcgag agttaccagc aggaaaaccg accgttgcct ggctccgtat
    13621 acagcgtgcc gacggtggac ggcggctggg cgggcgtgct cggctatctg atggtgatct
    13681 ggctgcagcc gtccctggag gcaaaccagg ccttcggctg ggattggcgc gaagccggac
    13741 accttcaggc aggcctcgtc gccgccggcg agtggtggcg ctgcatcact gcgctcaccc
    13801 tgcacggcga cctgggccac atcgtagcca actcgctgtt cggctcgctg ttcggcctgt
    13861 acgtcggccg gtacctcggc tccggcttcg gctggctttt gatcctcgcc agcggcgcgc
    13921 tcggcaacgc catgaacgcg tggatccagc cggacgcgtt cagctccatc ggcgcatcca
    13981 ccgcgagctt cggcgccctg ggcgtgagcg cggctttcgt gtggcgccgc ggctattacc
    14041 gcaaccgcga ctggcgccgc agccttgcgc cgatattcgc gggtatagcc ctgctggcgt
    14101 tcaccggcac cgggggggag aacaccgacg tggtcgcgca cttcaccggc tttgccgcgg
    14161 gcgccctcct cggcgccgca gccgccgcgt tcgacatccg tcgtctgggc gtctccgggc
    14221 agtatcttgc gggggccgcg gccatcaccc tcgtcgtgac ggcctggtcg ttcgccggca
    14281 cagtcggttg atcaccgcct gctgcgcacg gaggcagtca gaatcacccg catggcgtcc
    14341 tctacgccga tgtccaacgg cgacacccga tcttccggga ccatgagcgt gtagccgcca
    14401 atctggtaac tcatgggaaa gtagacggcg atccgccggc tgccgccttg gtccatgaag
    14461 gatggagagg ggccggtcac gaagccaatg accgagacgt catctgagac gtccacgctc
    14521 acgaccctcg ccgcgccgtc ggccggcccg ctggagaaga actggtaaaa gtcgctgatt
    14581 gccgtgtaca ccgttttgac cagcggaatt cggtccagga gattccgggc gtgccgccat
    14641 aggctctgca ggaaaaagat ctgagcgagc acgccgaccc cgtagaccat cacgatgccg
    14701 aatatgaggc ccaggcccgg caggtagagg ccctccggca ggatgaccag gaacaccgag
    14761 cgcagcaggg actccgtggt cacggccagc cagacgacga accagatggt cagggaaagg
    14821 ggcagcacga cgccgagacc gcgcaaaaag gtagtggtga tgtggctcat gacgatacga
    14881 ccttgaagtt gatgcagcga agtctagccg ttcgcgcgac gccgcgttca accgcgccga
    14941 tggtcggccc gacgggcggc cgccacttga gttaagctcc aggcaaaagt tgcgcaggag
    15001 ctgacgatga aactgggact ggctttaggg tattcgggcg cgtacctgaa catcccattg
    15061 aagaaggttc agctggccga gcggctgggc tacgactccg tctggacggc cgaggcctac
    15121 ggttccgacg cgataacgcc gctggcttac atcgcggctc acaccgagcg catccggctc
    15181 ggcaccggcg tcatccagct cgctgcccgt acgccggcca acgccgccat ggcgttcgcc
    15241 acgctcgatc agctggcggg cggcaaccgt gccatctgcg gtatcggggt ttccggcccg
    15301 cagatcgtcg agggctggta cggacagccc tggggacggc cctactaccg gatcaaggat
    15361 tacgtggcca tcatgaagaa gatcttcgcc cgggaagagc ccgtcaccca cgccggcaag
    15421 gagatcagcc tgccttacca gggcgagggc gccctgggga tcggcaaacc gctgaagtcg
    15481 atcctccaca tgaacccgaa catacccatc ttcctgggaa ctggcatgga gtccaccgtg
    15541 cgcctgacgg ccgaggtcgc cgacgggtgg ctgccgctgg gattcgtgcc cgaaaccgca
    15601 gcgctctacg atggctggat cgatgagggc cttgccaaag gcggcaagac gtcggagcag
    15661 ttcgagcggc aggcaggtac gtcggtccag gtgaccgacg acgtgaaaag cgctctggac
    15721 cggctcaagc caggcatcgc gctgtacgtc ggcggcatgg gccacaagac caagaacttc
    15781 cacaaggaga tgatgatcag gcgcggcttc ggcgaagccg cagagcggat ccaggagctc
    15841 tacctggcaa agcgcaagga cgaggccatc gccgccgttc ccgacgagtt cgtcgaccag
    15901 ggcgccctgg tgggtcccaa ggagcgcatc gccaggcgtt tccgggactg ggaggattcg
    15961 ggcctcaccg gtctgaccat ttccggggac gaggaagccg tgcgcttcat ggccgagtgc
    16021 gcgcggctga atttcgccgc cgagtgagcc cgcggcgcct agcggccgga agcgcccgct
    16081 gagcgccccg aacccagggc gtaccgcaga cccaccatca tcgagtgggt ctgctggtag
    16141 gcctccaccg ggccgccgtc cggccccgtc atcttcagat cgctcgaaat ccagtaccgg
    16201 tattccgccg acaccgtcag ccgctgggac acgctgacct ctgcacccag gcgaaactcg
    16261 gccgcgtaag attggtcctt gtcgtccacg aacagctcat cgagaacgtc tacgtcgtag
    16321 tcggcccaca cgagccccgc gccgacaccc aggtaagggc gggtccgggc ttccggaaag
    16381 aactggtaga tgccggacgc gagcaggctg gtggagcgga tccggcccga gccctgcttg
    16441 gaaccgaacc tgagcccgaa atccagaagc tcgacctcgt tcttccgaaa cgcggtttcc
    16501 agctcgaagc gccagcgccg gctgagcgcg taaccaaccc cgaacgccgc caccgggccc
    16561 acgtccagag catcgaaatt cgtctgaccg ggatcgatct cgacgtccac ggagaatccc
    16621 gcgcccgcaa aggtggtgag gtagaagccc cggggcgaag gccgagggga cgctgccggc
    16681 cgcgaccgct cgacgccgcc ggaacggtag cgcagctgca gccagcctga atgaacggca
    16741 tgtttcgtat cgagcttggt ccctgtgaac tccttcagct ttacggacgg cgcctgccag
    16801 taacggtact cgacccccag atccatcctt cgggtcaggg gcaggctgat ccccccgatc
    16861 agttgaaatg caaagctcgt ggcctggtcg tcgactaaag gatctctggg ctcgggatca
    16921 atctgcgcct ctatgctgcc atcggttatg tccagttccg tccaggccaa cccgacgcca
    16981 gcgccgaggt aagggcgaaa gtcccttccc accgcaaact cgcgcaccgc gttgatcgcc
    17041 aacgtcgtcg cggaaaaagc gttctcggga gcggggtcga gggcggcggc agacgccgat
    17101 accggcgcgg aaaacgacac gagctccacg tcgttgcgct gccgcgacgc ctcgacttcc
    17161 acccgccagt ccggcccgaa agcccaaccc agcgccgcag accagaccgg agcgccgaag
    17221 tcgtagtcga cgacgccgcc caactcgtcc agctccatgt tctcggcgtg attccagccg
    17281 atccccacgc ccgcatacca gccgccagcc tggtccgcgg cagcacagga gctccaaagg
    17341 agggcacacc ccagcatgga cgccagagcg ccctggcacc agtggcgact cacgccggga
    17401 tcgaccggcg accggatggt tgccgatacg gggtcatcgg gattcctcgc caagctctgc
    17461 acacagctcg cccatccgca gctcgccttc ccgggtttgc ccgccgatct ggaggaacct
    17521 gcgcatgttg cgctgggcgc cttccgtgcg cagcagccgc tgaaacaggt aggcttcgtt
    17581 cgagagccct tggtgaacgg gggtctcggc ccccagaacg gcctctttcg ccagcctcac
    17641 ggcctccacc gggtacgacg cgatccgtcg cgcaagcgcc gccacgaacc cgccgatttc
    17701 ctccggctcg aatatgcggt tcagatagcc ccagctttcc gccgtctcgg catcgagatc
    17761 agcgccgccc agcacgatct ccagcgcgcg cccccgaccc accagcctag gcagacgctg
    17821 tgttccggtc ccgccaggga gaatgcccag gggcacctcc atctggttga tcttcgttcg
    17881 accgcgcacc ccgaaacgca tgtcgaagct tgctgccagc tcgcttccgc caccgccgac
    17941 gcgcccttcg atctgcgcga tggtcacctt gtccatggtg cggaaccgct cgcacatgag
    18001 gtggtactcc gaaagctcgg cggagcgttg ggcctcaccg tccacgggga acgaaagaat
    18061 ggtttccacg tcgaaatgcg cgagaaagaa gtccgggtcc gcactcttca tcaccaccac
    18121 cgttaggttc gggtcggccc gaagctcggc gccgagactc gcaagctcgc ggtagagggc
    18181 cacggtgatg aggttgatgg gcggattgct gacggttacc gtcgctaccc gcccatcgat
    18241 gtccacgtcg agcagctcgt aatcgtaagc cacggatatc tcccttcctg gtgatcaaag
    18301 cgccgcggca ttcctctgga ggcgtgccgc acgatgcccc atactcgagg gcattatatc
    18361 gacctgaccg gaatatggac ggaagacagc aggccatcgt caaccgaatc gacgagctcg
    18421 ccgacgagct gctcgccgtg tcccatgcca ttcacgccaa tccggagctc gctttcgagg
    18481 agcacgatgc ctgcgcgctg ctggcaagca cggccaggcg gttcgatctt ccggtggaaa
    18541 cgggcgtttt caccctggaa accgccttcc agggaagctt tggcgggcgc acgtcggggc
    18601 ccaccgtggc gattctcgcg gagtacgatg ctctgcccgg gatcggccac gcgtgcggcc
    18661 acaacctcat cgcgacatcc gctctcggtg ccgctctggg gctcaaggcg gtggaagatg
    18721 agctgcccgg gaccgtccga ctcatcggga cccctgccga ggaacgcggg ggcggaaagg
    18781 aactgatggc gcgggcgggc gcattcgacg gcgtggacgc ggcgatgatg atccatcccg
    18841 cgggggtgaa tcttgcgacc atgccctgca tctgcgttgc cgaggtggag gtgatctatc
    18901 acggccgctc tgcccacgcc tcggcgatgc ctcacaaggg catcaatgcc ctcgacggcc
    18961 tcctgctcgc ctaccaggcc atatccaacc tgcgccagca catacgcgat cacgagcgca
    19021 tccacggcat cgtcaccgaa ggaggcctgg caccgaacat cgtccccgac cgggccgccg
    19081 gggccttcta cgtgcgagcc gccaacgaac aggatcttgc ccgcctgaag ccgcgcgtgc
    19141 aggcatgttt cgaggcaggc gcaaaaggga gcggctgcga attagaggtc aactgggcga
    19201 acgtcgacta cctggacctg aacaccaact ggcctcttgc caaccgcttc caggcccacg
    19261 gcgagcgtct ggggcgcgag tttctcaaac ccgcttccct cggcggcgcg ggcagcaccg
    19321 acatggggaa cgtcagctac cgggtaccat ccatacatcc gatgctggcc tgtgctccac
    19381 ccaacgtcgt catccacaac ccggagttcg ccaagtgggc tcgttccgag cagggcgacg
    19441 ccgcggccat cgacggcgcg aaagccctgg ctctgaccgc cgccgactac ctcctggatg
    19501 gctcgctgca ggcggaggtg aacaaagcgt tcgcgatctc gaagggcctg gcctgaccgc
    19561 ccgcccggcc tgatgaacgt ctggctgttc ggctacggat ccctcatctg gcgcccggag
    19621 atcgcttacg tggagcgccg ccccgcccgc ctccacggct gggcgcgccg cttctggcag
    19681 ggctcccacg accaccgcgg catgccccgc cagcccggca gggtggtgac cctcatcgag
    19741 gaggaaggcg gtcattgcga cggcatggcc tatctcgtgg acaggggcga tgtcgaagcc
    19801 atattcgcgg cgctggatca tcgagagaag aacggctatc ggcgcttcga cgtggaactg
    19861 catttctctc gctacaatcc tgcgccggac accacgggag tggtctacat cgcgcccccg
    19921 ggcaatcacg ctttcctggg acccgccccg cttgcggaga tggctcgcca gatcagcgag
    19981 tgcgcggggc cgagcggccg caacgtcgac tacctgctcg acctggccga agcgctgcgt
    20041 cagcttgacg cccaggacgc ccatgtgttc gagttggaag cggccgtccg gcacgtatcc
    20101 gggagcagat aatggacatt tccttcagcg cggagcacga acgcttccgc gacgaggttc
    20161 gggtttttct cgacggggcc ctggacgacg agctgcgcga agcccagcgc ttctgccccg
    20221 gcatcttcct ggattacgag cacaacatcc gctggcaccg catcctgcac gccaagggct
    20281 gggtcgcccc cgcgtggccc cgggaatacg ggggcaccgg ctgggacctg acgcgccggt
    20341 acatctggtc cacggagacc acccttgccg gcgcaccctc gctcgcgccg atgggccttg
    20401 gcatgtgcgg cccgatgctg atcggctacg gaaccgaagc ccagaaagct tattacctcc
    20461 cgcggattct ctccggcgag gactactggt gccagggcta ctctgagccg ggttccgggt
    20521 cggatctcgc ctccctcaag ctccgggcag acggcgacgg cgaccactac gtcctgaacg
    20581 gaaccaagat ctggaccacc cacgcgcatt acgccaatcg catgttctgc ctcgtcagaa
    20641 ccgacgacgg gggacgtccc cagcagggga tcacgtttct gcttctcgac atggacacgc
    20701 cgggcatcag cgtggagccc atcgtgttcg catcgggcac tcacgaggtc aatcaggtgt
    20761 tcttcgacga tgtcagggtt cccaaggcca accgtgtcgg tgaggagaac gacggctgga
    20821 ccgtggccaa gtacctgctc gagttcgaac gaggtggcgg cggcagcgcc gcctaccgga
    20881 cggccctgac ccgggttcgc aaactggccg gacaggttac cgccgatggc gcttccctgg
    20941 ccgaagaccc ggatttcaag cgggcgctgg acgaaaccga cgtaaagctg caggccattc
    21001 aatacaccga gtttcgcatc atggccgcgc tctcccgggg ccagaacccc gggcccgaat
    21061 cctccataat gaagaacctg ggcgccgaca tcggccagcg cctcaccgag ctgaccgtcg
    21121 aagccatggg ccagtacatc gcgccccatc agcccgaggc ccggcaggtg ggcagcaacg
    21181 tccccgtggt aggacccgcg gacggcgcaa ccgccatgcc gcgctacttc aacctgcgcg
    21241 ccagctccat cgctggaggc accaacgagg tgcagaagaa catcgtcgcc aagctggtcc
    21301 tcggcctgtg aacggggcgc tggccgacaa ctctcaaggg aagtgacatg agcgaactcg
    21361 tactgcggga ggaccgcgaa ggtctggcaa tcctcaccct gaacagaccg gaagccttga
    21421 acgcgctgag ccccagcctg tttgtggagc tgcgccgcca cgtggacgac atagcgcaag
    21481 atgtcgacaa cgtgggctgc gtgctgctgc gcggcgaggg aaggtccttc tccgccggga
    21541 acgacctgaa agccattcag gccggggagc gcgcgccggg cccgcacttc caggccgaaa
    21601 cgctggatgc catcgagggg ctgccacagc cggtcatcgc ggccgttcag ggccattgct
    21661 acacgggctc gctggagctt gcgctggcct gcgatctcat catcaccgcc cgcggcgcga
    21721 agattgcgga cacccacggc aagtgggcaa tgtcaccaac ctgggggatg agccagcgcc
    21781 tgcctagaag agtcggcctg gctcgcgcca aggagctcat gtacagcggc cgcgtcatcg
    21841 acggtgagga ggcggccgcc atcggtctag cggtgcgctg cgtgccggat gaggaactgc
    21901 tcgaggcagc cgtcgccatg gcccgcacgt tcctggagaa ctcctggttc acgctgcgag
    21961 ccgacaagat gctggtgaac ggcgggttga accacacgct agccgacggc cttgcgttcg
    22021 aacgaagcaa cagcccaggc agggggcccg atctcgagga gcggctcgag cgcttcggca
    22081 agtgagaccg gtggtggccc gtcgtcagcc gagctcgtcg atgctcgaga tgattcgacc
    22141 cacgatgccg tactccaccg actcttcggc gctcatccag tagtcgcggt cggtgtcctc
    22201 ttccacccgt tcgaggggct ggcccgtctg ctccgcgatg atggcgttga tgcgctgacg
    22261 ggttctgacg atctcgcggg cgtggatctc cacgtccgat gcgctcccgc caaagccgcc
    22321 cgcaggctga tggatgagaa accgggtgtt ggggaggcag aagcggtcct cgcggtcggg
    22381 cgcgaggtag atgtgcgttg cgatgctccc gacccagccg gtgcccacgg ctttcacgtt
    22441 cggcttgata taccggatca tgtcgaaaat cgtatcgccc gactccacgt gcccaccggg
    22501 ggatccgatg tagagcacga tggggtcgtc cgactcgtat gccagcgcca gaagtttctc
    22561 cgccgtgcgc tgggcgacgt ccttgttgat ctccccgaac agggtcaagg ttcggttctt
    22621 gagcagcgtg ttctccagaa actgaaactg cttggctacc tccgccgctt cttccacgcc
    22681 atcaagcctc gtcatccggt gtctccagat atctttcggt ttaaacttgg ggcgcattct
    22741 acacggggga tacgaatgcc gttagcgccg gaataccaga gttttctcga gcagctcgcc
    22801 caggtgccgg gacctcccct gcacgaactg tcgcccgccg atgcccgcgc cgcttaccgc
    22861 ctcttgcggc cgagggacga gcagatcacc gtcgccgcgg tgaacgagct ggccgcgccc
    22921 gggccagccg gatccattgc gctgcgcgtc taccgtcccc agggagatgg cccgtttccc
    22981 atgctggtga actttcacgg cgggggctgg gtattcggcg acctcgatac cgccgatacc
    23041 gtctgccgca atctctgccg aaacacgggg tgcctggtcg tctccgtgga ttaccggctg
    23101 gcgcccgagc atccctaccc cgcggccatc gacgacgcgt acgccgccac ttgctggctg
    23161 gcggaacacg cgagcgagct ggagggcaac ggccgcctgg cagtggccgg cgaaagcgcg
    23221 ggcggaaatc tggccgccgc cgtgagcctg cgcgcacggg aggaaggcgg tccggccatc
    23281 gactatcagc tgctcttcta tcccgttatc gatgcggact tcaaccgggc ctcctacgag
    23341 gagaaccgga gcggctacct gctcgagacg gcaacgatga tctggttctg ggaccactac
    23401 gtgcccgata ggtcacgccg ttctcaggcg tacgcttcgc ccattcgggc agacgatctg
    23461 ggccgcctgc cgccagcgct gatcctgacc gcggagttcg acccgctccg ggacgaaggc
    23521 aacgctttcg ccgaggcgtt gaaagccgcg ggcacgccgg cagagctgat ctgctgcgcc
    23581 ggcctgattc acgacttctg cggcaccgcg catcttttcg agtcgagccg aaagccgttc
    23641 gaggatgcct gtgccgcctt gcgccgggcc atgagcggca cgcccgaaag ctgaagccga
    23701 acacccccgc aaaactgaag cctgaccgag gaagaagcat gctttacgtg atgatctgcg
    23761 aagacaaacc ggacagcgag gcgctgcgtc tggcgaaccg ggccgaccat ttggaatggg
    23821 tgggcgggaa gacggcgatg atacggctcg ccggccccat gctgagcgac gacggtgagc
    23881 acatgcgcgg atccctgttc atcatccagg ccgagtcagc cgatgcggtg agacagttca
    23941 atggggagga tccctacacg cgcgcggggc tctggggcaa ggtgaccatc cggcccttca
    24001 gacaggtcct gccagcagat tcatgaggcg acccagacca tgagaaaagc cgtcatcctc
    24061 ggcgtcgggc ccgacctcgg gctcggcgcc cagctgtgca aacgatttgc cggcgagggt
    24121 ctccacgtga tggtcgccgg acgcacggat acttccgtgg aggcagtggc cggggacatc
    24181 cgcgccaatg gcggcgccgc caccgccgta gtggcggacg cgacttcaga ggacgatgtc
    24241 cgcgcccttt tcgaccaggc cggcgacgac ctcgacctgg ccatctacaa cgccggcaac
    24301 aacatgcccg gccgcatcat cgacatggaa gccgactact tcgagaacag ctggcgcgtg
    24361 ggctgctttg gcggtttcct tttcggccgg gaggccgtcc gccgcatggt tccaaaaggg
    24421 gccgggaccc tgctgttcac cggtgcgagc gcttcgctgc gcggtcgcgc caagttcggc
    24481 gccttcaact cggccaaggg cgccctacgc aatctggctc aggcaatggc caaggagtac
    24541 ggggcagacg gcatccacgt gggtcacgtg atcatcgacg gacccatcgc gggagacaag
    24601 ataatcaaag ccttcccgga gtacgcgcag aggctggggc cggagggcat gatcggcatc
    24661 gaaggtatcg tcgagggctt cgccttcctg taccgccaac cgcgcaacgc ctggacgttc
    24721 gaggtggacg tgcgcacttc cctggagaag tggtaacccg ggcagacccc ctgagcccat
    24781 cggcgagacc acgaaggtcc accgggcctc atggcctctg ccccgttcag tcgacgcggg
    24841 cttctgcgta accggaaagc accaccgcgc catcctgatt ggtggtttcc acgttcagct
    24901 tcacgatgcc gtcggccact tcgtccaccg tggcggtcgc gttcagcgtg tcgcccggcc
    24961 agacctggtt ggtaaagcgc acgccgtact tggtgagccg tccgtccccc acgtagttgg
    25021 tcagcattcg gccggtcatg cccatggtca gcatcccgtg ggcgaaaacc gacgggtagc
    25081 cggcgatctg cgtcgtgaac acctcgtcgg agtgcagcgg gttgtaatcc cccgatgtgc
    25141 cggcatacat gacgatctgg gtccggctca ggttctcgac gagacactcg gtgtaggtgt
    25201 cgccggcgtt gaggtcgctt gcgttcagtg ccatctctcc ctcccttatc ggtccaccgg
    25261 gcgctcggtg cgcacaccta cgccgcgggc agtgatcacc agctcaccgt tctgatcccg
    25321 atactcggtg atcgtctcac tgaacagcag cttgcccgcc cgtttgctcg ccttctccca
    25381 ggtcttgccg ggctttacct ccgcgctcag gacgtcgccg ggcttcaggt gccggtgata
    25441 ctcgaagtgc tgctcggcat gcaggccgcc gcctccaccg cccccgctgc cgccgccttc
    25501 gcgcttgacc ccggtggctt ctttggcaga gccgaaccag ggctcaccga tcttcggccg
    25561 gaggaaataa tccggatcga actgggcgct cgcctgggcg aacgtcggtg gggcgatgat
    25621 ggtgcccggc tcggttcctt tcgcgtactc ctcgtcgtag tagatctgat tgtcgtcggc
    25681 aatcgagcgt gcaaacatca tgatgtggct tgcctcgatc gggaatctgt ccacggacat
    25741 cgtcgtttcc ccctcgtgtt cgttgtgcgc gttggcgctc tacccctcgc cgatatacgg
    25801 cgtctcagag caaaggagcg gaagtatccc ccaatggtct gggggggatc aacgaccctt
    25861 gaattccggg tcgcgcttct ccagaaaggc cgtgatgcct tctactttgt catcggtctg
    25921 aaccaacgcc ccctgggcat acttctcgaa catgagcgcg ccggccaggc tcgcctccat
    25981 gccgaaatgc accatcgcct tcatcgtgcg gggcacgaag ggggcgaagc cggccaggtg
    26041 gtcggccatg cggcgtgcct cagggatcag ctcggccggc tcaacgactc gcgtcacgag
    26101 acctatctgc agagcccttt cggcatctac cggctcgccc atgagcagca tctccatccc
    26161 ccagcccctc cccacgattc ggggcaggcg ggccgtggcc ccgccaccgg ggatgatgcc
    26221 cagctttcct tcgggggtcg caaagcgcgc cttcggcacc gcgatcctca ggtcacagcc
    26281 gagagcgact tccagacctc cacccatgca gatcccgttg atggccgcga tggtcggctt
    26341 cggcatgcgc tccagctggt ccgccaggcc ctgcacgatg ggctcgagcg ccttcctgaa
    26401 atcgcggtgc aggacctcag agaggtcgga tcccgaagca aaggccttat ccccggcgcc
    26461 ggtgatggtc agcaccctca ccgcgtcgtc cgccgcggcg aggcccaccg cctgatgcag
    26521 ctcgtctagg gtggccaggg agatcgcgtt gtgcttgtcc gcccggttga tggtgatgag
    26581 ccccatgggg ccctggactt cgtagaggat gttctcgaat tcgctcacgt gcctggttcc
    26641 gttgtttggc ccatcgtctg ctgcgcgggt cagccgtcga ccagctgcat ctcttcgatg
    26701 aaggcctggg attgcgcaat ggacgcttcc atctcgcgga tgaggcccgc cacgtccgct
    26761 tctatggagg ttagctcgct tttcagggac gcgatggccc gggcgttgag attgtgcttc
    26821 aaaaacagaa cgtggtcccg gaaagtgtcc agcaccggct ccattttccg ttccgcccgc
    26881 cgcattccct tgatcagctc gtcgtacttt cgctgtgagg ctcgcagctg gctggcgctg
    26941 gcgcttttga gcttcgggtt gccgatcagc tcgatctcct cactccactc gtcgaaaaga
    27001 tcttccgaga cgtcttcgac cgaatcgata cggtcccgaa cccgctcggc tcgatcctcc
    27061 gccgaatcgt aggcatccga cagttcctcg taggtgtcct ttagtctcga tggctctaca
    27121 gcaaccacgc tggcgaactg ctctaaagcg tctgcgaact cctccttggc gtcttgctgg
    27181 gcttccacgg cgtcctcgac gcgatccacg agtatgtcgc gcttgtaaac gccaacctgc
    27241 tccatcgcac cgtaataggc cgtttcgcag cctgctgacc ccagcgctac gagcccggct
    27301 acaagcgccg ttttccaatt cgccaccagc cttctcccaa cccgatacaa accctttcca
    27361 cgacccgacc aatctcaacg aactcgggaa cacggtgctg aacgctcagg tcgggctgtt
    27421 gtttctcgcg ccgatcctta ggacaatcgc gtcccaaatc aggcgctcaa cgcagaagcg
    27481 cgcgcatagc gtgcccgagc cccatcaaaa caacaaccac tgaaacacgg gaggccacac
    27541 cccatggagg agatttccac agagcagatg actgcttaca ccgacaccgc catcgagctg
    27601 ctgatgacct acggacccaa gttcctgttg gcgctcgtcg ttctcatcat cggcctgtgg
    27661 atcatcaagc gcgtcgtcaa agtcacgtca aaggcgatgg agcgtgggaa cgccgagccc
    27721 acccttgcga agtttgtcgc aagcctcgtc tccgtaggct tgaaagccct gttgatcatc
    27781 agcgtggcat cgatgatcgg catagagacc acgtccttca tcgccattct cggtgccgcc
    27841 ggcctggccg tcggtctggc tctgcagggc accctcgcga attttgcggg cggcgctctg
    27901 atcctgctct ttcgccctta caaggttggt gactggatcg acgctcaggg cgttgcagga
    27961 acggtgcacg ccattcagat tttcaacacg attctgaaga caccggacaa ccggaccatc
    28021 atcatcccga acggcgccat atcgaacggc atcatcacca acaactccac cgagccgacc
    28081 cgccgcgtgg acttcgtgtt cggcatcggc tacggggacg acattgccaa agccaaaggc
    28141 atcatcgaga ggctgatcgg ggccgacgag cggacccttt ccgaccccgc ggctcagatc
    28201 gtggtttccg cgctcgccga cagctcggtc aacctcaccg taagggtctg ggtcaacgcc
    28261 gccgattact ggggcgtgtt cttcgatctc accgagaagg tcaagctcgc attcgacgag
    28321 gagggagttt ccattccgtt ccctcagcag gacgtgcaca tgcaccaggc aggctgacgc
    28381 tcgctggggt gttttcgggc gccgcctgac cagcggcgct cgagcaaaaa aaaacgccgg
    28441 cttggagaga aagccggcgt ctaaaggacc ttttacaagg tctcaagggg atagagattg
    28501 cctcatgggg aagcggttta acccgcttcg aggtccaata tgagggaaag acgtttcgcc
    28561 gcgatttcct gccgcgacct ttttgtgaca ttttgtttcc cgatgcccac cggttcgacc
    28621 tagagcgtaa caaagacgtc gatctcgccg ttgtccgctt cctgaatggc cactcgccag
    28681 ccctgcgcct ggcaaagctg ctggacgatg gccagcccga ggccgctgcc acccgtggct
    28741 acactgcgtg aatcgtcaag gcgaaagaaa ggctgaaaga cccgctcccg gtactcttcg
    28801 gggatgcccg gcccgctgtc gaggacgtgc acctcccggc cggcaatgtg gacgcgcacg
    28861 cccttgccgt gctgcaaggc gttggtgatc aggtttatca acacacgctg cagcgcgccc
    28921 ggggcgacgc tgacgcgaac gtcgtctccg tcactggtca gcgggatgga ctggtcgaag
    28981 ctggcaacca catcctccac gaagggcttg agctccacct cctgcgccac ctcggaagtc
    29041 ccccgcgcaa atcgcagggc gtcggtgatc agctcgtcca tggattcgag attgcgctca
    29101 aatcgctcaa cgagcttggg gtcgacattg tccggcagca attcgagcgc cagccgcatt
    29161 cgcgtgaggg gggtgcgcag gtcgtgggag atcccggcaa gcagcgtcgt gcgattggct
    29221 agcagagcgg tgatctcacg cgccatggtg ttgaaattgc gggtcagcga gaccagctcc
    29281 cgaggcccac gctccggcag cggctcgaaa ttctcgccac cgcgataggt ctcggcgcgt
    29341 tcggcggtct cgaccagagg gcgcgcgatc ctgagcacga tgagcagcga cgtgatgaac
    29401 accacggcag caccaagccc gacgatgatg atcccgacgt aaaggggctg gatgtcgcgc
    29461 cggttggacg cgaagcctat ctggatgtca tacccgccca tgggcacggt gacccagatg
    29521 aggtcatcgc cctcacgcag gcgagccggc gtgttcaggc gctggctgag cttctcctcg
    29581 accagagaaa ggtagggctg gtccaggtct gcgaaaggca gatcgcgaac ctctgcgctc
    29641 acgatcagat cgtgattctg cgccagctcg atctcgaagt acgggcgggc tgccggcggc
    29701 aactctaccc aggtctgggc agaaagcacg agcagggccg cttcgtcgtc cgccgagcgc
    29761 tccgcgatgg gctcgatgac gaaaacgttg agcgcggcga tggcgatcag cgcgatgacc
    29821 agcgaaccga ctgccaggat gaggttggtc cgtgcgagca gagacgccgg accccagagc
    29881 ccccgccgcc cctcaggcac ggccatcggg gcagaacatg taccctttac cccagatggt
    29941 cttgatgaat accggcgtgg agggatccgg ctcgattttc gcccgcagcc gggtcacccg
    30001 cacgtcgatg ctgcgatcga agggcgaccg ctcggctccg gtcagcagat ccaggatgcg
    30061 gtctcggtcc agaaccttgt tgggatggga gacgaggatc gcgagcagat cgaactctcc
    30121 cgacgtgagc ggtaccgtct tgccatctgc cgtcagctga tgagccgaaa ggtccagctc
    30181 gaagctcccg aaacacacgc ggttctctgc ggacgacgcc tgcccggttc gctgagcgcg
    30241 gcgcagcacg gctcgaaccc gggcaagcag ctcgcggggg ttgaacggct tgccgatgta
    30301 gtcgtctgcg ccaacctcga gcccgacgat gcggtcgatc tcttcgccct gggcagacac
    30361 gattatgatg gggacatcgc cgtcggtctt gagccggcgg gcgatcgaaa ggccgtgctc
    30421 accgggcagc atgagatcga gaatgatgag atccacctcg ttggattcca ggtaggcgtc
    30481 catggcctcg ccgctgtcga cgcagtccac ctcgaatccc tgctgggaga gataggcctg
    30541 tgccagctca cgaatctctg ggtcgtcgtc aacgactaga agcttggcac cttcagcatc
    30601 cacggcgggg gcttacggtg agggatcctt gaccgtgatg accggcggcg actgctcctc
    30661 ccgctggccg tctgcggtcc tttgctcgaa gcctacgccg taggtttgat tgcccgccgc
    30721 gccggggcgg actcgaacca cgttggtctc gatccgaacc acggacccgt ccggattgcg
    30781 gatgatgcgg ccgtaacggc gctgaaacgt gatgcggatg gtgccgtccg gaccgcgctg
    30841 gcgcgccata cgcatcttga cctcggtcaa cagggcccgg cgctcggcgg tgtccaggga
    30901 ttcccactgg gcggcgagag ccgtcagctc ctggtcggat ttggctgcat agacgttcgg
    30961 gatgctggcc gcatcgcccg cacgcacagc ctcgtcggcg gcgaaagcgc cgccggacag
    31021 tgacgtgaag agcaaaacgc tcagggtcag gcttctgatg aacgtaaccc ctgttccatt
    31081 cggagctccg accgcatccg cgcaccggat gctcccattt tccgccccgt gatgattgga
    31141 ccactataaa tgcttatggt gccggcagcc tctacgtgtc ctacaagaac gcgcacaggc
    31201 ctcgcgaatt atccccagag cgacggcaat tctgactctg cgctgtttca gcgccatttc
    31261 ccttgggttt caatttgtta cttaaccccg ggggctgatc aggctgcctc gaacagacct
    31321 gctgcaccct ggccaccgcc aatgcacatg gtgacgactc cgtacctctt gccacgcctt
    31381 ttcagctcgc ggagaaccag gcccgtcatg cgcgagccgg tcatcccgaa cggatggccg
    31441 atgctgatgc tgccgccgtt gacgttgtaa atctcgttgt cgatgcccag cgcatcccga
    31501 caatacaggc actgcgacgc gaacgcctcg ttgagctccc acagatcgac gtcatcctgc
    31561 ttgagacccg cgttttccag cagccgcggg atggcgaaca ccgggccgat gcccatctcg
    31621 tcgggctcgc agccggccac ggtgaagccc cggtagatgc ccaacggttc taggcccagc
    31681 tgcgctgcgc gctcagcgct catgagcagc gtcatggacg cgccgtcgga gagctgagaa
    31741 gcatttcccg cagtgacgga gccatcttcc tcgaaggccg gcggcagcga tgccagacct
    31801 tccagggtgg tggtggggcg gttgcactcg tcccggtcga cgacgacgtc cacgcggctt
    31861 tcttcacccg tctccttgtc caccttgagc atggtggccg ccatgggcac gatctcttcg
    31921 gcgacatatc cggcctcagc ggcggccgcg tatctttgct ggctctgcag ggcatactcg
    31981 tcctgggctt cgcgggtcac ctgatagcgc cgcgcgacca cctcggcggt attccccatg
    32041 gccatgtaga tgtccggttt ctcctccacc agccgcttgt tgagctcggc cttggccgcc
    32101 tcctgacggg tacgcatgga gatcgagtcg acgccgcctg caatggcgac ctgagtctgg
    32161 ccggtcgcaa tctggttggc ggcgatggcg atggactgca ggccggacga gcagaagcga
    32221 ttgatggtca ggccggcggt cgtgatcggc agcttcgaca gcaccacggc cagtcgggcc
    32281 aggttgccgt cctgctcgcc gacgtggcta gccgccccca ccacgacgtc ttccacctcg
    32341 tgcggcgata cgccgccgtt cctgtcgagc agcgcgtcga tgcagtgcgc gagcatgtcg
    32401 tcggagcggg tgaggttgaa gctcccacgg aaggatttgg cgagcccggt tcgaacgcta
    32461 tcgacgatga ccacgtcttt catgatgttc ccctttcaag ttgttcgctc aacgcaggcc
    32521 ctgggttttg cagcgccccg acaaaagtgc cccgggagcg taccagaaaa aaaaagacac
    32581 ggtctcggct ggctgggccc gcaccctcgt catacgcgcg ccaatgacgc cgaccgcttc
    32641 gctgcagggc aaacagaaac ggcccgaggc tggcgcaccg cgccgcgttc ggaccgttac
    32701 ctgatcacga gcgggccaga cttcgaacag ttcgaaactg cactgacccg ttggctggaa
    32761 gtggaaggcg gctagttgga gcccggcggg gtgatgttca tctcccccat ggccgattcg
    32821 gctgcctctt ctgcctccgc ggctgcttct tccatcatct cggctgcctc tgctgcagct
    32881 tcggtggcgg tttcgtcagc ggcttcctca agcgcttcca tcccctcgtc catagcctct
    32941 tcggctgctt cagcggcagg ctgaatgaac ccacccgcgg tctcatccgc ggctgcctct
    33001 tccgccgcgg cgggcgcatc agcgggtccg gaccctgcgt cctggccgca gccgacaaac
    33061 ccgacgagaa cgacaacgcc gagcagcgcc atcatccagt tcttgatcgt actcatctat
    33121 ccctcctcgt tgctttcgac ccagggccag accctgcagg ctgagcagac gcaggcctta
    33181 ttgccaccgt cattccccag cggtcgaggt attcgcaaga ctacagaaaa aggtctggcc
    33241 aaccaacctg tacctgtcgg cgtgctaagc taagcggcca cctctggagg gtccatggaa
    33301 acgatcactt tggtcgaagg cctgtgcttc ggcgaggggc cgcgctggcg agacggccgt
    33361 ctgtggctgt ctgatatgca cgctcaacag gtgatcgccg tggacggcga cggccacgtt
    33421 caggtcgtgg tcgaggtgcc gaacctcccc tccggcctgg gatggctccc ggacggcgac
    33481 ctcctgatcg tctccatgcg cgatcggcgc gttcttcgcc acgacggcgc ccgcctgcac
    33541 acccatgctg acctgtcaca gctggcaagt ttcgactgca acgacatggt ggtggacgtc
    33601 cacggtaggg cctacgtggg aaacttcggt ttcgatcttc acggcggaga agcgcccagg
    33661 gccgcggagc taatctgcgt ggaacctgac ggaaccgcga ggatcgtcgc agacgacctg
    33721 ctctttccca acgggacggt gatcacgccg gacggcaaca cgctgatcgt tgcagaatcc
    33781 tggggcggcc gactgaccgc tttcgacatc gaccaggccg gcaacctgtc gaaccgcaga
    33841 gactgggcca agctccccga cggcgccgtg cctgacggca tctgcctgga ttccgacggg
    33901 ggaatctgga ctgcctcgcc cgcgaccaac gagtgcctgc gccagatcga agggggcacc
    33961 gtcacgcacc gggtcccggt gaaccagggc gcttttgcgt gcatgctcgg gggagacgac
    34021 ggccgctccc tcttcatcct caccgccggg tcatcggcgc ccgacgcctg ccgtgcggcc
    34081 cgctccggac gcgtggagct cgttaccgcc ccgttcccgc acgccggctg gccgtaaaag
    34141 ccgcgcaacc gactcacacg aggaaacaag acgtgctgta cgaaaactac gagtcaccct
    34201 ggctgaacga tgagcttcgc ctgctccagg acgccgcagc gaagtttttt cagcaggagt
    34261 tcgtgccgct gaacgagaag tggatcaacc agggcaaggt ggaccgtgaa gcctggtaca
    34321 aggccggcga ggcaggcctg ctctgcgcgg agatgcccgt cgagtacggg ggcggtggcg
    34381 gcgactaccg ccacgagacc gtcatcatgg aggagcagct cgccaccggg gtgaccggct
    34441 tcggcaacca ggtccacagc agcatcgtcg ccccgtacat cctgcattat ggctccgaag
    34501 accagaaaca gcgctggctg ccgaagatgg ccaccggcga gctcgtcggc gccatcgcca
    34561 tgaccgagcc caatacgggc tccgatcttc aggccgtgcg cacgtcggcg atccgcgacg
    34621 gtgacgagta cgtcatcaac ggctccaaga cctacatttc caacggccag cacgccgatc
    34681 tggtcatcgt cgtggcaaag accgatccgg cccagggcgc gaagggcata tcgctgatcg
    34741 tggtggagac gggcaagagt ccgggtttcg agcggggacg caacctcaag aagctgggac
    34801 agaaggccgc cgacacctcg gagctgttct ttgccgactg ccgggttccc gtcacgaacc
    34861 gcctcggcga ggaggaagga cgaggcttcg tccagctgat gcagcagctg cctcaggaac
    34921 gcctgaacat cggccaggca gccgtggtgg ccatggaacg cgccatcggg atcacgctcg
    34981 acttcgtcag ggagagacgc gccttcggtc agcggatcct ggatttccag aacaccagat
    35041 tcaagctgtc
//
LOCUS       DBNHLM010000323         3921 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1066358, whole genome shotgun sequence.
ACCESSION   DBNHLM010000323 DBNHLM010000000
VERSION     DBNHLM010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1066358"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="AC16QF_14935"
     CDS             complement(<1..828)
                     /locus_tag="AC16QF_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450 family protein"
                     /protein_id="HIR6449904.1"
                     /translation="MSDPASYNLMEDETRECPFPYFNAIRHEQPVYFMEELGAWYVSR
                     YEDVRHVKKHPEIFSNNIFEHGGHRGAERNIAEEYKSEHGWRRVSTLQRTDPPVHSKY
                     RKLINHAFTVSRIRKMTGYVETVVNDLIDQFIDKGEVDLVWEFAIPLPCTVIADQLGV
                     PRDKIWRLKEWSDAMLAPGGGFVDEAAALKCGQLVVEAQKFFAEVMEARRAEPRDDII
                     TDLVQAKVVDEPGGPERPLDMFELQDLLDQLLTGGNETTTNAIGSAMMLLLQRPGLME
                     "
     gene            928..1791
                     /locus_tag="AC16QF_14940"
     CDS             928..1791
                     /locus_tag="AC16QF_14940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6449905.1"
                     /translation="MKIGVFATFMSPLGTAEMIQDFGRRAEAAGLDSIWMGEHVVLFD
                     EMEFPYPGSRDGRIPVPEGGGLLDTVATFGLLAGSTSRLRFGTGICLVPQRNPIYTAK
                     EFATLDWLSGGRIDFGIGVGWCKEEVIACGYGWEDRGERCDEFLTLMRKLWSEPVVSY
                     SGKHVNVQGCRMDPKPIQKPHIPIIVGGHTPRAMRRAAEFGAGWYGFGITPDQTSAVL
                     TELDAALGAAGRSRDGFEIIITPASEDADSMAAFADLGVDRLILHLGSQRPEKLAARV
                     DQLASLVQAAA"
     gene            1877..2758
                     /locus_tag="AC16QF_14945"
     CDS             1877..2758
                     /locus_tag="AC16QF_14945"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6449906.1"
                     /translation="MARLAGKNIVVTGSSRGLGRAFAIAMAAEGASVVINGTNREALA
                     EVEKTISSAGGNVLAVPGSVTEETTCAQLVSGCVETFGSIDVMINNAGIVRDRTLLKM
                     TVEEWDDVLAVNLRGAFACTKHAALAMREAGGGHIVQVISASGLSGGFGQGNYAAAKA
                     GMMGLLRTAVLELSRFNIRNNALWPVAETDMTQVVFERAAAMSREQGAEPPTPAEMGF
                     GKPEEVAQGLVWLVSDGAAHFDGQCMSFNGRKTAIWRHPAEEHVSFHDEPWSFDELND
                     YYRDVKPLPIYRPVRAE"
     gene            complement(2768..3382)
                     /locus_tag="AC16QF_14950"
     CDS             complement(2768..3382)
                     /locus_tag="AC16QF_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449907.1"
                     /translation="MKAWALLLGIYGVFVYWYTSFQGPLSEAEIETYLAALPEGGGND
                     ERLALWRAFMESDTGDDFVMINVVDLRDRPLAMEGVSPDETAADVLARYTAPFLGAAI
                     RSAAHPILYGNAAAPALDLWGIDGAERWSNGGLVRYRSRRDLMDQVVRTSRLDIHEFK
                     IAAMEKTIAYPLDPWWHLGDPRLLLALVLGCIGFGLQARGARGR"
     gene            complement(3408..>3921)
                     /locus_tag="AC16QF_14955"
     CDS             complement(3408..>3921)
                     /locus_tag="AC16QF_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6449908.1"
                     /translation="SQVFGWIFFLAGPLGPILIALLARRLGERGRGDANITAGMIGGL
                     ISVPTILLIQFVPSPMWAFVLYVPAMIFINSPFGIAAGSLPVITPPNMRAQVAALYML
                     TVSVGMMLGPPVAGAFNEYVFPEAEGVRYSLITLTSLFGAIAGLSLWLGRRAYAVSLA
                     EAEEQAAREG"
BASE COUNT          701 a         1299 c         1286 g          635 t
ORIGIN      
        1 ctccatcagc cccggccgct gaagcagaag catcatggcg gacccgatgg cgttggtggt
       61 ggtctcgttg ccgcccgtca ggagctgatc gagcagatcc tggagctcga acatgtccag
      121 cggacgctcg ggaccaccgg gctcgtcgac aaccttggcc tgcacgagat cggtgatgat
      181 gtcgtcgcga ggctcggcgc ggcgcgcctc catgacctcg gcaaagaact tctgggcctc
      241 gaccaccagc tgcccgcact tcagcgcagc cgcctcgtcc acgaacccac cgccaggtgc
      301 gagcatggcg tcggaccatt ccttgaggcg ccagatcttg tcacggggaa cgccgagctg
      361 gtcggcgatc accgtgcagg gcagcgggat ggcgaattcc cagaccagat ccacctcccc
      421 cttgtcgatg aactgatcga tgagatcgtt gaccacggtc tccacgtatc ccgtcatctt
      481 gcggatccgg ctgacggtga aggcgtggtt gatcagcttg cgatacttcg aatgcaccgg
      541 cggatccgtc cgctgcagcg tggacacgcg ccgccagcca tgctcgcttt tgtactcttc
      601 ggcgatgttc cgctccgcgc cgcggtggcc gccgtgctcg aaaatgttgt tcgaaaagat
      661 ctcggggtgc ttcttcacgt ggcggacgtc ttcgtagcgg ctgacgtacc acgcgccgag
      721 ctcttccatg aagtagacgg gctgctcgtg ccttatcgcg ttgaaatagg gaaacggaca
      781 ctctcgcgtc tcgtcctcca tcaggttgta actcgccggg tctgacatgc gctctcccac
      841 caaacacaca ttcaaccgct gaatcctacc agcaaagcgg gcggcgaacc cctagaatct
      901 gtagaacaga acagggaggc aactgctatg aagatcggag tgttcgcaac cttcatgtcg
      961 ccgctgggca cggcggagat gatccaggac ttcggccgtc gggccgaggc ggcgggtctc
     1021 gattccatct ggatggggga gcacgtggtt ctgttcgacg agatggagtt tccctacccg
     1081 ggatcccggg acggtcgcat cccggttccc gagggcggcg ggctgctcga caccgtcgcc
     1141 accttcgggc tgctggcggg aagcacttcc cggctgcgct tcggcactgg tatctgcctg
     1201 gtcccccagc gcaaccccat ctacaccgca aaggagttcg ccaccctgga ctggctgagc
     1261 ggcggacgga tcgactttgg catcggggtg ggatggtgca aggaggaggt aattgcctgc
     1321 gggtacggct gggaagaccg gggcgagcga tgcgacgagt tcctcacgct catgcgcaag
     1381 ctgtggagcg agccggtggt cagctactcc ggcaagcatg tgaacgtgca gggatgtcgg
     1441 atggacccca agccgatcca gaaaccacac atccccatca tcgtcggcgg ccacacccct
     1501 cgcgccatgc gccgggccgc cgagttcggc gcgggctggt atggtttcgg gatcaccccg
     1561 gatcagacat ctgccgtgct gaccgagctg gacgcggcgc tgggggcagc gggccggtca
     1621 cgggacggct tcgagatcat catcaccccg gcgtcggagg acgccgacag catggccgcc
     1681 tttgccgacc tgggcgtcga tcgcctgatt ctgcacctgg gctcacagcg cccggagaag
     1741 ctcgccgcgc gggtagatca gctggcgtca ctggtccaag cggccgcctg aaccggccgt
     1801 gccggccggt ggtgagcgct ctcgtggcac aatggggccg cggggggatc ctcaaccaga
     1861 ctggagacat ttgctcatgg cacgccttgc agggaaaaac atcgtcgtca ccggctcgtc
     1921 gaggggctta gggcgcgctt tcgccatcgc catggccgcg gaaggagcca gcgtggtcat
     1981 caacggcacc aatcgggagg ccctggccga ggtcgagaag accatatcct ccgcgggcgg
     2041 caacgttctg gcggtccccg gatccgtcac cgaggagacc acctgcgcac agctggtgtc
     2101 cggctgcgtg gaaaccttcg ggagcatcga cgtcatgatc aacaacgccg gcatcgtgcg
     2161 cgaccggacg ctcctgaaga tgaccgtcga ggagtgggac gacgtgctgg ccgtcaacct
     2221 tcgcggcgcg tttgcctgca ccaagcacgc ggcgctcgcc atgcgcgagg ccggcggtgg
     2281 ccacatcgtc caggtcatct ccgcgtcggg cctctccggc gggttcggtc agggcaacta
     2341 cgccgcggcc aaggcgggca tgatgggcct gctgcgcacg gccgtgctgg agctctcccg
     2401 cttcaacatc cgcaacaacg cgctgtggcc ggtggcggag accgacatga cccaggtggt
     2461 gttcgagcgc gcggccgcca tgtcccgcga gcagggcgcc gagcccccca caccggcgga
     2521 gatgggcttc ggcaagcctg aggaagtcgc ccaggggctc gtgtggctgg tcagcgacgg
     2581 ggccgctcat ttcgacggcc agtgcatgtc gttcaacggc cgcaagacgg ccatctggcg
     2641 ccacccggcc gaggagcacg tgtcgtttca cgacgagccc tggagcttcg acgagctcaa
     2701 cgactactac cgggacgtca aaccgctacc catctaccga ccggtgcgcg ccgagtaggc
     2761 ggatcgctca gcggccccgg gcgccccgcg cctgcagccc gaagccaatg cacccgagca
     2821 ccagggcgag cagcagacgc ggatcgccca ggtgccacca ggggtccagc gggtaggcga
     2881 tggtcttctc catggcggcg atcttgaatt cgtggatgtc caggcgactg gttcgaacca
     2941 cctgatccat gagatcccgg cggctgcgat agcgcacgag cccgccgttg ctccagcgct
     3001 ccgcgccgtc gattccccat agatccaacg cgggggccgc cgcgttaccg tagaggatcg
     3061 ggtgcgctgc gctgcggatg gcagctccaa gaaacggagc cgtgtaccgg gccaggacgt
     3121 cggcggcggt ttcatccggc gaaaccccct ccatagcaag cgggcgatcg cgcagatcga
     3181 cgacgttgat catgacgaag tcgtcacccg tgtccgactc catgaacgcg cgccacaagg
     3241 caagccgctc gtcgttcccg cccccctccg ggagcgcggc caggtaggtt tcgatctccg
     3301 cctcgctgag cgggccctgg aatgacgtgt accagtagac gaacacccca tagattccca
     3361 gcaggagagc ccacgccttc actttcgatg cctcgttttg atcagggtca cccctcccgg
     3421 gccgcctgct cctccgcttc ggcgaggctg acggcgtagg cgcgccggcc cagccagagc
     3481 gagagcccgg caatggcccc gaacaggctg gtcagcgtga tcagcgagta gcgcacgccc
     3541 tccgcctcgg ggaagacgta ctcgttgaat gcccccgcca ccggggggcc cagcatcatg
     3601 cccacggaca ccgtcagcat gtagagcgcc gccacctggg cacgcatgtt gggcggcgtg
     3661 atcacgggaa gagaaccggc ggcgatgccg aacggggagt tgatgaagat cattgccggc
     3721 acgtacagca cgaaggccca catcggcgag ggcacgaact gaatcagcag aatcgtcggc
     3781 accgaaataa gcccgccgat catgccggcc gtaatgttgg cgtcgccccg gccccgctcg
     3841 cccagcctgc gggcgagcaa ggcgatgagg atggggccca gggggcccgc cagaaagaat
     3901 atccacccga acacctggga c
//
LOCUS       DBNHLM010000324         3687 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_49157, whole genome shotgun sequence.
ACCESSION   DBNHLM010000324 DBNHLM010000000
VERSION     DBNHLM010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_49157"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="AC16QF_14960"
     CDS             <1..765
                     /locus_tag="AC16QF_14960"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6449909.1"
                     /translation="EVVTRLILRASEHVEPAQGTLAGYREREEKRLRELVHEANNPLS
                     VVYNYLHILELRLQHEPSAVEQLRMIGEELKRTGDIIRRARELPPVEEPELSGTVEIS
                     AVDVNRLVGQVFELHRGYAADRGVTLELEAAPGALVAASDESRLLQVLTNLVRNAIEA
                     APDGSVTIGSLGGVFRDGVEGVELSVADSGPGLPREVLERLGDPKQSAKGGDHAGLGL
                     HIVHRLVEEVKARIDVRTSTGSGTTFTVFLPLSQPV"
     gene            848..2026
                     /locus_tag="AC16QF_14965"
     CDS             848..2026
                     /locus_tag="AC16QF_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008367295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR6449910.1"
                     /translation="MPALEPFVWDDPLLLEDQLTEDERMVRDTAREYAQRELMPRVLK
                     ANREEHFDREIMNEMGALGLLGATLPAKYGCSAVNYVSYGLMAREVERVDSGYRSAMS
                     VQSSLVMHPIYAYGNEDQRERYLPKLASGEWVGCFGLTEPDHGSDPGGMVTRAEPVSG
                     GYRLNGAKMWITNSPIADLAVVWAKLDGVIRGFVVERAFDGFSTPKIEGKMSLRASVT
                     GEIVLDDVVVPEENLLPNVKGLAGPFGCLNRARYGIAWGSMGAAEFCWHAARTYTLDR
                     EQFGRPLAANQLIQKKLADMQTEIALGLQGCLRMGRLLDEDRLAVENISMLKRNNCGK
                     ALEVARMARDMHGGNGISDEYHVIRHVMNLETVNTYEGTHDIHALILGRAQTGLQAFS
                     "
     gene            2166..2828
                     /gene="uvrY"
                     /locus_tag="AC16QF_14970"
     CDS             2166..2828
                     /gene="uvrY"
                     /locus_tag="AC16QF_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HIR6449911.1"
                     /translation="MIRVMVVDDHKLFRLGLRRMLADVKGIHVIGEADCGEAAVRLAR
                     AERPNIVLMDILMPGIGGIEATRRIARLEMDIKVIAVTGCHENPFPMQMLRAGACGYL
                     SKGVDRDELVAAIRKVFLGRRYLSADIAQEVAASAFDQTRESPFEQLSNREMQIMMMV
                     VNCHKVNDISEDLHLSPKTVNSYRYRIFEKLNVSSDVELALMAVRHGMVIPQLNGMAR
                     AV"
     gene            2818..3456
                     /locus_tag="AC16QF_14975"
     CDS             2818..3456
                     /locus_tag="AC16QF_14975"
                     /inference="COORDINATES: protein motif:HMM:NF015146.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR6449912.1"
                     /translation="MRYDRLESCRICPRLAAHLDQVRQAHPGYRCLPVGATGSVRAKL
                     LMVGLAPGLHGANRTGRPFTGDASGTFLFAALARVGLASDADPLVARLVGARLTNAVK
                     CLPPQNRPNAAEMSACSRYLREEIDELWSPKVRSARCVLCLGRLAHRSVEIALGKRLP
                     AFLHGQLCEVKPGLWLADSYHPSRQNTNTRRLTPEMLDSVLENVKGVVGLTG"
     gene            3493..>3687
                     /locus_tag="AC16QF_14980"
     CDS             3493..>3687
                     /locus_tag="AC16QF_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008369689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR6449913.1"
                     /translation="MTAFDSEAFLGTLTRKPGVYRMLDADGNVLYVGKARNLRSRVSS
                     YFRASGLATKTMALVGRIADI"
BASE COUNT          664 a         1129 c         1224 g          670 t
ORIGIN      
        1 gaggtggtga cccgtttgat ccttcgggcg tcggaacacg ttgaaccggc gcaaggcacg
       61 ctcgcgggct atcgggaacg ggaagaaaaa cgtctgcggg aactcgttca cgaggccaac
      121 aacccgctga gcgtggtcta caactatctc cacatcctgg agctcaggct gcagcacgag
      181 ccgagcgcgg tagagcagct gcgcatgatc ggcgaggagc tcaaaaggac gggcgacatc
      241 attcgccgcg cgcgcgagct cccgcccgtg gaggagcccg agctgtcggg caccgtggag
      301 atcagcgcgg tggatgtgaa tcgcctcgtc gggcaggtct tcgagctgca ccgcggttac
      361 gccgccgatc gcggggtgac gctcgagctg gaggcggcgc ccggcgcgct ggtggcggcc
      421 tcggacgaaa gccggctgct gcaggtgctc acgaacctgg ttcgaaacgc catcgaggct
      481 gcacctgacg gcagtgtcac cattggcagc ctcggcggcg tctttcgcga cggtgtcgag
      541 ggtgtcgagc tttccgtggc ggacagcggt ccgggacttc cgcgggaagt cctggagcgt
      601 ctcggcgacc cgaagcagtc tgccaaggga ggcgatcatg cgggtctcgg tcttcacatc
      661 gttcaccggc tggtggagga ggtgaaagct cgaatcgacg ttcgtaccag tacgggcagc
      721 ggaaccactt tcaccgtctt ccttccgctc tcccagccgg tctgatatgc ctggccaggc
      781 gccggttgcc tagcggccgt atcgctccta cccttgggcc tgactttccg caaactggga
      841 tgagcccatg cctgctctcg aaccgttcgt ctgggacgat ccgttgctcc tggaagatca
      901 gctgaccgaa gacgagcgca tggtccgcga tacggcgcgt gaatacgcgc agcgcgagct
      961 catgccgcgc gttctcaagg ccaatcgcga agaacacttc gaccgggaaa tcatgaacga
     1021 gatgggggcg ctcggtctgc tgggtgccac gctgccggcg aagtatggct gttcggccgt
     1081 caactacgtc tcttacggcc tgatggcccg ggaagtggag cgcgtggatt cggggtaccg
     1141 gtcggccatg agcgtgcaat cctcgctggt catgcatccg atatacgctt acggcaacga
     1201 ggatcagcgt gagcgctacc tgccgaagct ggccagcggt gagtgggtcg gctgtttcgg
     1261 cctgacggag cccgaccacg gatcggaccc cggcggcatg gtgaccaggg ccgagccggt
     1321 gtcgggcggc taccgtctga acggtgcgaa gatgtggatc accaactcgc caatagccga
     1381 tctggcggtg gtctgggcaa agctcgacgg cgtgatccgc gggtttgtgg ttgagcgcgc
     1441 gttcgacggt ttcagcaccc ccaagataga gggaaagatg agcctgcggg cgtcggtcac
     1501 cggggagatc gtgctcgacg acgtcgttgt tcccgaagag aatctgctgc ccaacgtcaa
     1561 aggcctggcc ggtcccttcg gttgcttgaa ccgcgcgcgc tacggcatcg cctggggatc
     1621 catgggggca gccgaattct gctggcatgc ggcacgcacc tacacgctcg atcgggagca
     1681 gttcgggcgc ccgctcgccg ccaaccagct gattcagaag aagctcgcgg acatgcagac
     1741 ggagatcgcc ctcggcctgc agggctgcct caggatgggt cgcctgctgg acgaggatcg
     1801 tcttgccgtc gagaacatct ccatgctcaa gcgcaacaac tgcggcaaag cgctggaggt
     1861 cgcgcgcatg gcgcgggaca tgcacggcgg caacgggatc tccgacgagt accacgtgat
     1921 ccggcatgtc atgaatctgg agacggtcaa cacctacgag gggacccacg acatccacgc
     1981 gctgatactg gggcgcgccc agaccggtct gcaggcgttt tcctgatcgc atctgatatc
     2041 agcgcgacat cggaaaatgg aaatttcgtg aaaacccggc agattctcac cccgcgccgt
     2101 tgttagccgc ctggcgcccg cctatcgtca ctgcttcaga tgctggacac tttgttgggg
     2161 gcaagatgat cagggttatg gttgtggacg atcacaagct gtttcgtctg gggctgcggc
     2221 gcatgctggc ggacgtcaaa ggtatacacg taatcggcga agcggactgc ggggaggcgg
     2281 cggtgcggct cgcccgcgcg gagagaccga acatcgttct catggacatc cttatgcccg
     2341 gtatcggcgg catcgaagcc acccggcgga tcgcgcgtct ggagatggac atcaaggtga
     2401 tcgccgtgac cgggtgccac gaaaaccctt tccccatgca gatgctccgg gccggagcct
     2461 gcgggtatct cagcaagggc gtcgaccgcg acgagctggt cgcggccatc cgaaaggtct
     2521 ttctcggcag gcgctacctg agcgccgaca ttgctcagga ggtggcggca agcgcgttcg
     2581 accagacccg ggagagcccg ttcgagcaac tttccaatcg tgaaatgcag atcatgatga
     2641 tggtggtcaa ctgtcacaag gtgaacgaca tctccgagga cctgcacctt tcgccgaaga
     2701 cggtgaacag ctatcggtac cgaatcttcg agaagctcaa cgtctccagc gacgtggagc
     2761 tcgccctgat ggcggtacgc cacggcatgg tgatacccca gctgaacggg atggcccgtg
     2821 cggtatgacc gcctggaaag ttgccgcatc tgccctcggt tggccgctca tctcgatcag
     2881 gtacggcaag cccatcccgg ttaccgctgc ctgcccgtgg gcgccacggg gtctgtgcgc
     2941 gccaagctgc tgatggtcgg gctcgcgccg gggctgcacg gcgccaaccg cacgggacgc
     3001 ccctttacag gggacgccag cggcacgttt cttttcgcgg cgcttgccag ggtcggattg
     3061 gccagtgacg ctgatcctct cgttgctcgt ctcgtcgggg cccggttgac gaacgccgtc
     3121 aaatgcctgc cgccgcagaa ccggcccaat gccgcggaga tgtcagcctg tagccgctac
     3181 ctgcgcgagg agatcgacga gctgtggtct ccgaaggtgc gatcggcgcg ctgcgtgctg
     3241 tgccttgggc gcctggccca tcgctcggtg gagatcgctc tcggaaagcg tctgcccgca
     3301 ttccttcatg ggcagctctg cgaggtgaag ccgggcctgt ggctggccga ctcctatcat
     3361 ccaagccgtc agaacacgaa cacgcgtcgg ctcacacccg agatgctcga ttcggtcctg
     3421 gaaaacgtga aaggcgtcgt cgggttgacc ggctgattgc ggcccatggg cgcggactct
     3481 atagttccgc cgatgacggc cttcgactcc gaagctttcc ttgggaccct gacgcgcaaa
     3541 cccggcgttt atcgcatgct cgacgccgac ggaaacgttc tgtacgtggg caaggcccgg
     3601 aatctcagga gccgggtcag cagctatttc cgtgcttcgg gactcgcgac caagaccatg
     3661 gctctggtcg gccgcatcgc ggacatc
//
LOCUS       DBNHLM010000325         3558 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_528082, whole genome shotgun sequence.
ACCESSION   DBNHLM010000325 DBNHLM010000000
VERSION     DBNHLM010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_528082"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="AC16QF_14985"
     CDS             <1..855
                     /locus_tag="AC16QF_14985"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR6449914.1"
                     /translation="QGLQKEFGPERVYDTPISEEGIVGLGVGAAATGCRPIIDLMFMD
                     FLGECMDEVSNQLAKMRYMFGGHATLPVTMLTMAGAGASLAAQHSQSLEAWICHLPGL
                     KVCMPSSPYDAKGLIISAARDDNPVIVVLNKMSLAITGEVPEEPYAIPLGEANIVRPG
                     GNFTLIATGRMVYEAMTAAEKLKDLGIDTEVIDPRTIQPFDTQMVIESVKKTHRVMIV
                     HEAVRFGGIGGEIAAQIQELAFDYLDAPVARVGAPFSPVPFSPALEAVYVPNADRIVA
                     EAKKLLGL"
     gene            918..1841
                     /locus_tag="AC16QF_14990"
     CDS             918..1841
                     /locus_tag="AC16QF_14990"
                     /inference="COORDINATES: protein motif:HMM:NF012981.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR6449915.1"
                     /translation="MARASVFDNQEKQAIVRRILVGMREAGCNEVAYLDDGHGIVRTA
                     LDDVREPFIRKPVATTETANALDTINAARALRAEGCDVVLTLGGDGTNRAFALGWPDP
                     PLIPISTGTNNVFPVLVEATIAGAAAGLIASGQISLADVATQHKAIHVTIVGERDDLA
                     LIDAVLSSERFVGARALLAPETLQTALVTRADPAAVGITSIGGLLKPVADAEDGGLLM
                     QLGGTGGSVVNAPIAPGYYRRVRVSELRRVGFAETIEVTGPGVLAFDGERERSLKPGQ
                     RATMSVHRSGPWVVDVTKTLMLAAEKGCFRR"
     gene            1896..3254
                     /locus_tag="AC16QF_14995"
     CDS             1896..3254
                     /locus_tag="AC16QF_14995"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR6449916.1"
                     /translation="MPTEIFLVKVGMNMTEGVVDEWYIPDGGAVEVGELLYRLETEKV
                     NLDVDAEVAGTVKHLVGEGVTMKPGDVVGYVYAPGEEIPDDLPAAGAATPAPAAETAE
                     TTAPAAAAPAAITRGDGERILSSPAARRLAGELGVDISTLAGTGPGGRIVEADVQAAA
                     AGAGEERGSRSSPMARKVARELGVDIATVTGTGPGGRITKEDVELAAKAPPAAAPAAR
                     EAVASRGDQTVPVRGMRKTIAARMHESLQSMAQLTMDMDVIMDDAVKLRGQLIEEWAD
                     EGVRPTYTDLVIRAAAKALEKHPLMNSVFGETEMTLKGEVNVGMAVALEEGLIVPVVR
                     HANVLNMKELARESSRLAAACRDGNLTPDDLQGGTFTVSALGMFGVDSFTPIINSPQV
                     GILGVNRLRDDVAWDGAVPVKRQAMRLSLTWDHRALDGAPAAQFLATVRDLLEAPYRL
                     LV"
     gene            3264..>3558
                     /locus_tag="AC16QF_15000"
     CDS             3264..>3558
                     /locus_tag="AC16QF_15000"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6449917.1"
                     /translation="MTITVRPLTPVIGAEVSGADLTDLGDQTFADIDRALTEHLVLFF
                     RDQPRLEPDVQIAFARRFGDLHIHPAAPHLEGYPEIFVIHTDRNSKVANGNGWH"
BASE COUNT          621 a         1208 c         1197 g          532 t
ORIGIN      
        1 cagggcctgc agaaggagtt cggacccgag cgggtttacg acacacccat ctccgaggag
       61 ggcatcgtcg ggctcggcgt cggcgccgcc gcgacgggct gccggcccat catcgacctg
      121 atgttcatgg acttcctcgg ggagtgcatg gacgaggtga gcaaccagct cgccaagatg
      181 cgctacatgt tcggaggcca cgcgacgctt cccgtcacca tgctcaccat ggccggcgcc
      241 ggcgcgagcc tggcagccca gcattcccag agcctggagg cgtggatctg ccacctgccg
      301 ggcctgaagg tctgcatgcc ctccagcccc tacgacgcca aggggctgat catctctgca
      361 gcgcgggacg acaatccggt catcgtggtg ctgaacaaga tgtccctggc catcacgggc
      421 gaggtacccg aggagccgta cgccatcccc ctcggggagg cgaacatcgt caggccgggc
      481 gggaacttca cgctcatcgc caccgggcgc atggtctacg aagccatgac cgcggcggag
      541 aagctgaagg atctcggaat cgacaccgag gtcatcgatc cacgcaccat tcagccattc
      601 gacacccaga tggtcatcga gtcggtcaag aaaacccatc gggtgatgat cgtgcacgag
      661 gccgtgcgct tcggcggcat cggcggcgag atcgccgcgc agatccagga gctagcgttc
      721 gactacctcg atgcgcctgt tgcgcgggtt ggcgcgccct tctcgccggt gccgttcagc
      781 ccggcgctgg aagccgttta cgtgcccaac gccgatcgca tcgtagccga agcgaagaag
      841 ctgctgggct tatagcgacc ctgggcacca taggcatcat cgcgaacccg gcgtcgggca
      901 aagatgtccg gcgcctggtg gcgcgtgcgt ccgtcttcga caaccaggaa aagcaggcca
      961 tcgtccgccg catcctggtg ggcatgcgcg aggcgggctg caacgaggtc gcctatctgg
     1021 acgacggcca cggcatcgtt cgcacggccc tggacgacgt gcgcgagccc ttcatccgca
     1081 agccggtggc caccacggaa accgccaacg ccctggatac catcaacgcg gcccgggcac
     1141 tgcgagcgga aggttgcgac gtggtgctca cgctcggcgg cgacggaacc aatcgagctt
     1201 tcgcgctcgg ctggccggat ccgcccctga ttcctatctc cacgggaacc aacaacgtct
     1261 ttccggttct ggtggaggcc accatcgcag gcgccgctgc ggggctcatc gcctccggcc
     1321 agatctcgct cgccgacgtg gcaacccagc acaaggccat acacgtgacc atcgtgggcg
     1381 agcgcgacga cctcgccctc atcgacgccg tgctgagcag cgagcgcttc gtcggcgccc
     1441 gtgcgctcct tgcgccggag acgctgcaga cggcgctggt tacccgggcc gatccggcgg
     1501 cggtgggcat cacgtccatc ggcggcctcc tgaagcccgt ggccgacgcc gaggacggcg
     1561 gcctgctgat gcagctcggc ggcacgggcg gcagcgtggt caacgcgccc atcgcccccg
     1621 gctactaccg gcgggtccgg gtgagcgagc tgcgtcgggt ggggttcgca gagacgatcg
     1681 aggtgaccgg acccggcgtg ctggccttcg acggcgagcg cgaacgttcc ctcaaacccg
     1741 gccagcgagc gacgatgtcc gtccaccgct ccggcccgtg ggtggtcgat gtcacaaaaa
     1801 cccttatgct cgcggctgaa aaaggctgtt tcaggcgcta ggatgtcgcg ttcggcgcgg
     1861 gtgacgccga agaacaacga aaggaaggtt tcgcaatgcc caccgagata tttctggtaa
     1921 aggtcggtat gaacatgacc gaaggcgtcg tcgatgagtg gtacataccc gacggcggcg
     1981 ctgtagaggt cggcgagctg ctctaccgcc tggaaaccga aaaggtgaat ctggacgtcg
     2041 acgccgaggt ggccgggacg gtcaagcacc tcgtgggcga gggcgtgacc atgaagcccg
     2101 gcgacgtggt cggctacgtc tacgcccccg gagaggagat tcccgacgac cttcccgccg
     2161 ccggcgcagc aacgcccgcg ccagccgccg agacggccga gaccaccgcc cccgcggccg
     2221 cagcaccggc cgcgataact cggggcgatg gggagcgcat cctgtcctcg ccagccgcca
     2281 ggcggctggc cggcgagctc ggcgtcgaca tctcgaccct ggccgggacc ggtccgggcg
     2341 ggcggatcgt cgaggcagac gttcaggcag cagctgcggg cgcgggcgaa gagcgcggca
     2401 gcagatcgtc gcccatggcg cgcaaggtgg cccgggagct cggtgtggac atcgccacgg
     2461 tcaccgggac ggggcccgga gggcgcatca ccaaggaaga cgtggaactg gccgccaagg
     2521 cacccccggc ggccgccccg gcggccaggg aggcggtggc ctcgcgtggc gaccagaccg
     2581 tgcccgtccg cggcatgcgc aagaccattg ccgcccgcat gcacgagagt ctccagtcca
     2641 tggcccagct gaccatggac atggacgtga tcatggacga cgcggtcaag ctgcgcggcc
     2701 agctcatcga ggagtgggcc gacgaaggcg tgcgcccgac gtacaccgac ctggtgatcc
     2761 gtgccgccgc caaagccctg gaaaagcatc cgctcatgaa cagcgtgttc ggcgagacgg
     2821 agatgacgct caagggcgag gtcaacgtgg gcatggccgt ggctttagag gaagggctga
     2881 tcgtgcccgt cgtccgccac gccaacgtgc tcaacatgaa agagctcgcc cgggaatcga
     2941 gccggctggc ggccgcatgc cgggacggca acctcacgcc cgacgatctt cagggcggca
     3001 cgttcacggt ctcggccctg ggcatgttcg gcgtcgactc ctttacgccc atcatcaact
     3061 cgccccaggt gggcattctc ggggtcaacc ggctgcgcga cgacgtggcg tgggacggcg
     3121 cggtgcccgt caaacgccag gccatgcggc tgtcgctgac ctgggaccac cgcgcgctgg
     3181 acggcgcgcc ggcggctcag tttctggcaa cggttcggga tctgctggag gcgccctacc
     3241 ggcttctcgt ctgaggtgtc caattgacca tcaccgtcag gcccctgaca cccgtgatcg
     3301 gcgcggaggt ctcgggagcg gatctcaccg atctcggcga ccagaccttc gcggacatcg
     3361 atcgggcgct caccgagcac ctggtgctct tcttccggga tcagccgcgt ctcgagcctg
     3421 atgtccagat cgcgttcgcc cgccgcttcg gcgacctgca cattcacccc gcagcccctc
     3481 atctggaggg ctacccggag atcttcgtca tccataccga tcgcaactcc aaggttgcca
     3541 acggcaacgg ctggcaca
//
LOCUS       DBNHLM010000326         6577 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1606229, whole genome shotgun sequence.
ACCESSION   DBNHLM010000326 DBNHLM010000000
VERSION     DBNHLM010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6577)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6577
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1606229"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..279)
                     /locus_tag="AC16QF_15005"
     CDS             complement(7..279)
                     /locus_tag="AC16QF_15005"
                     /inference="COORDINATES: protein motif:HMM:NF040598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpp/OprI family alanine-zipper lipoprotein"
                     /protein_id="HIR6449918.1"
                     /translation="MKNLFFAMIATLLLAGCATTEQVEALQNRVSALENQIGSAHDAA
                     GDAASAASRAASDAAAAQSTAERALDAANAANERAERISETCCSRT"
     gene            complement(370..1446)
                     /gene="amrS"
                     /locus_tag="AC16QF_15010"
     CDS             complement(370..1446)
                     /gene="amrS"
                     /locus_tag="AC16QF_15010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HIR6449919.1"
                     /translation="MDQTIVPTRYWHTLDDGRTQCDVCPRHCRLREGQRGLCYVRQCL
                     DGEVKLVSYGRSSGFCVDPIEKKPLNHFYPGSSVLSFGTAGCNLACKFCQNWDMSKSR
                     EMDTLADRAMPERLAEAAVDLACRSVAFTYNDPVIFLEYAVDVADACREVGVKSVAVT
                     AGYVDPEPRREFFSAVDAANVDLKAFSERFYHKICGGHLAPVLETLEFIRHETNTWLE
                     LTTLLIPGENDSDEELDEMTGWVVEHLGPDVPMHFTAFHPDWKMRDVPSTPPGTLTRA
                     RSIARRNGVHFAYTGNVHDPAGSSTYCSACDALLIERDWYQLGRWGLDGAGRCSACGE
                     PLAGHLEGPPGQFGSRRIPVRLDQ"
     gene            1507..2292
                     /gene="amrB"
                     /locus_tag="AC16QF_15015"
     CDS             1507..2292
                     /gene="amrB"
                     /locus_tag="AC16QF_15015"
                     /inference="COORDINATES: protein motif:HMM:NF013992.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HIR6449920.1"
                     /translation="MVSRSPAVADLFYPGDPAELAGTVRAYLDRADAPTVALRPKALI
                     VPHAGYAYSGPVAATAYRTLEPFRHEVERVILMGPAHRVYLEGLAVPETDAFAVPTGN
                     VPVDVEAVARLLELPGVLASDQAHAAEHCLEVHLPFLQAVLDRFSIVPVVVGACSSEL
                     VADVLDLLWGGDETLIVVSSDLSHYHPYEVAQALDAATAGEILARACNLTGEQACGAV
                     AINGLMLAATGHDLQVRELDLRNSGDTAGDRDRVVGYGAFALD"
     gene            2279..2905
                     /gene="amrA"
                     /locus_tag="AC16QF_15020"
     CDS             2279..2905
                     /gene="amrA"
                     /locus_tag="AC16QF_15020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HIR6449921.1"
                     /translation="MPSTDYGPDARQALLGIARDAIRIGLERHGVADPMSVVDVPSLL
                     PELLEPRATFVTLTQAGSLRGCTGSLEALRPLALDVVENACHSAFRDPRFPPVQAGEL
                     NRLRIELSVLSPLERMRVASEGELLERLEPSMGLVIEEGGRRGTFLPKVWESLPDPRV
                     FLDELKAKAGFPAGHWSDATEVFYYRTQTFAEPVAAFGSVVSHGGDSV"
     gene            2930..4108
                     /locus_tag="AC16QF_15025"
     CDS             2930..4108
                     /locus_tag="AC16QF_15025"
                     /inference="COORDINATES: protein motif:HMM:NF047238.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR6449922.1"
                     /translation="MNRSLRGRVAVVGVGETMYYKAGQSPDAEFKLALKAILAACEDA
                     GIDPKDVDGFASYSNDRSDPSRLAAALGCRELRFANMQWGGGGGGGSGALGNAAAAVA
                     TGMADCVVVFRALAQGQFGRFGQGPRRNVIAGDMAHVVPYGLMSPAQMFAMKVQRFMH
                     DHGVEQSALRAISLASYHHAQNNPRAVMHGRPLDEATYDASRWIVEPFHLYDCCLEND
                     GAAAMIVVPADRAGDCRPVPAYLLGAVSGSHYRAGASVHNTPDYATSTFKTLAPRLYE
                     MAGVVPADVDVLQSYENFTGGVLMSIVEHGFCEAQRCNEFFVPERLTAPDGDLPLNTS
                     GGNLAECYMHGLGLNIEAVRQIRGESTNQVMGAEISMVISGPMVTPVSACILGSEAAL
                     "
     gene            4105..4548
                     /locus_tag="AC16QF_15030"
     CDS             4105..4548
                     /locus_tag="AC16QF_15030"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6449923.1"
                     /translation="MSYYLRPGLPQPAPLPDGLDRPYWDGLREDRLMLQRCAQCGGWQ
                     WGPEWICHHCLGFELAFEAVTPRGIVYSYERVWHPVHPALAEQGPYLVVLVEIPQADG
                     VRLVGNLLGDPLQDVEIGSAVEAVFEHHEDVDEPFTLLQWRLLAD"
     gene            4575..5735
                     /locus_tag="AC16QF_15035"
     CDS             4575..5735
                     /locus_tag="AC16QF_15035"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6449924.1"
                     /translation="MIEIHGYCDERFSPVREVFARNFADKDEVGASLALTVEGEMVVD
                     MWAGHRDAAKSLPWEQDTIVNVWSTTKTMSALCALVLADRGEIDLYAPVTRYWPAFGQ
                     NGKEKIEVRHFMSHSAGLAGTDEPIRGEALYDWERVVNALERQAPWWEPGTASGYHAI
                     TQGHLIGEVVRRVTGMSCGTFFRREIAEPLGADFHIGTAPEHFSRIGDLLPPGVSLGG
                     ALAHEPSGRGGGEGRAASIAVRAFRSPFVRAEDAATAEWREAEIPAANGHGNARSVAR
                     IQTVIANGGVSAGTRILSEAGCRAVFDEQTHGRDLVLKAPLTFGMGYALLHGQSPREL
                     AFWGGWGGSSVVVAPQSRMCIAYVMNRMEPSLLGDDRGVSLNRAALAVMGRG"
     gene            complement(5738..>6577)
                     /locus_tag="AC16QF_15040"
     CDS             complement(5738..>6577)
                     /locus_tag="AC16QF_15040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HIR6449925.1"
                     /translation="KFEYPAHPKLTAHPTLWKVLPDARELVTANVERLSELQERLYAN
                     NRYSVLMIFQGMDAAGKDSTIKYVTSGVNPMGFQVVNFKQPTYKELDHNYLWRYWRAM
                     PERGRIGIFNRSHYEEVGVARVHPEIIERQNLPSEHVNGDFWENRFEDIRAMERHLHT
                     NGTIVLKFFLNVSKKEQKARLMKRLTNRDKHWKFDPRDIDERVHWDDHMKAYEAAIAA
                     THFDHAPWYVIPADDKWTMRVIVAEVLCTHLEQLRLSYPPAKGAHKHALAEALSRLEA
                     EKE"
BASE COUNT         1030 a         2051 c         2231 g         1265 t
ORIGIN      
        1 ggagcactac gtccgggagc agcaggtctc cgaaatccgc tccgcccgct cgttggccgc
       61 attggcggcg tccagcgcac gctccgcggt ggactgagca gccgcggcgt cgctcgccgc
      121 tcgggaagca gcagaagcgg catctccggc agcgtcgtga gcggagccaa tctggttctc
      181 cagcgcgctg acgcggttct ggagagcctc gacctgttcg gtagttgcgc agcctgcgag
      241 cagcagggtg gcgatcatgg caaagaaaag atttttcact tgggggcctc actcagccag
      301 gtttttctcg gaagcgaacc ctaccatagt cagcggcttt gtcatcagtt gattgcgctg
      361 aaattatgct cattgatcga gtcgcaccgg gatgcgccgg cttccgaact ggcccggagg
      421 cccttcgaga tgtccggcca gcggctcgcc gcaggcgctg cagcgtccgg ccccgtccag
      481 tccccagcgg cccagctgat accagtcgcg ctcgatgagc agggcatcgc aggccgagca
      541 gtaggtgctg gagccggcag gatcgtgcac gttgccggtg taggcgaagt gcaccccgtt
      601 gcgcctggca atgctgcgag cccgggtcag ggtgcctggg ggcgtgctgg gcacgtcgcg
      661 catcttccag tccgggtgaa aggcggtgaa gtgcatgggc acgtcgggac ccaagtgctc
      721 gacgacccag cccgtcatct cgtcgagctc ctcgtcggag tcgttctcgc ccggtatcaa
      781 cagcgtggtc agctcgagcc aggtgttggt ctcgtggcgg atgaattcca gcgtctccag
      841 aaccggcgcg aggtggccgc cgcagatctt gtggtagaag cgctcgctga acgccttgag
      901 gtcgacgttg gcggcgtcca ccgcggagaa aaactcgcgc cggggctcgg ggtccacgta
      961 tccggccgtc acggccacgg acttcacgcc gacttcgcga caggcgtcgg cgacgtcgac
     1021 ggcgtactcc aggaagatga ccgggtcgtt gtaggtgaaa gccacgcttc ggcacgccag
     1081 atccaccgcg gcttcggcca gccgctccgg catggctcga tcggccaggg tgtccatctc
     1141 gcgggacttg ctcatgtccc agttctggca gaacttgcag gcgaggttgc agcccgcggt
     1201 gccgaaggag agcacgctgc tgccggggta gaagtggttc agcggtttct tctcgatggg
     1261 gtccacgcag aaccccgacg atctgccgta gctgacgagc ttcacctcac cgtcgagaca
     1321 ctggcgcacg tagcacagtc cccgctggcc ctcgcgcagg cggcagtggc gcggacaaac
     1381 gtcgcactga gtgcggccat cgtccagcgt atgccagtag cgcgtgggca cgattgtctg
     1441 atccatggtt gctttatggg gtcggtaccc ccatatacca agcgccttaa caatcttccg
     1501 aatgccatgg tcagcagatc gcccgccgta gcggacctgt tctatcccgg agatccggcc
     1561 gagctggccg ggacggtgcg ggcgtacctg gaccgcgcgg atgcgcccac ggtcgccctg
     1621 cgaccgaagg cgctgatcgt gcctcacgcc ggctacgcat actccgggcc ggtggcggcg
     1681 acggcgtatc ggaccctcga gccttttcgg cacgaggtgg aacgtgtgat cctcatgggc
     1741 ccggcccatc gggtgtatct ggaaggcctg gcggtccccg agactgatgc gtttgcggtc
     1801 ccgacgggta acgtgccggt ggacgtcgaa gccgtggccc gactgctcga gctccccgga
     1861 gtgctggcct ctgaccaggc ccacgccgcc gagcactgcc tggaggtgca cctgcccttc
     1921 ctccaggcgg tgcttgatcg tttttccatc gttcccgtgg tcgtcggcgc gtgctcgagc
     1981 gagctggtgg ccgacgtgct cgacctgctc tggggcggag acgaaaccct catcgtcgtg
     2041 agttccgatc tgtcccacta tcatccctac gaggtggctc aggcgctcga tgcggcgacg
     2101 gcaggcgaga ttctcgctcg cgcctgcaac ctgaccggcg agcaggcgtg cggagcggtc
     2161 gccatcaacg gtttgatgct ggcggctacc ggtcacgacc ttcaggtgcg ggagctcgac
     2221 cttcgcaatt cgggcgatac ggccggcgac cgtgatcggg tggtgggtta tggcgcgttt
     2281 gccctcgact gattacggcc ccgacgcgcg acaggccctg ctgggcatcg cgcgggacgc
     2341 cattcggatt ggtctcgaac gccatggcgt cgcggacccg atgtccgtgg tggacgttcc
     2401 cagcctgctt cccgagcttc tcgagcctcg agcgacgttc gtcaccctga cccaagccgg
     2461 gtcgttgcgc ggttgcacgg gttcgctgga ggcgttgagg ccgctcgcgc tcgatgtggt
     2521 ggaaaacgcc tgccactccg catttcgcga cccgcgcttc cctccggttc aggcaggcga
     2581 gctgaaccgt ctgcgcatcg agctttcggt tctctccccc ctggagcgga tgcgggtagc
     2641 cagcgagggg gagctcctgg agcgtctcga gccgtccatg ggtctggtga tcgaggaagg
     2701 cggccggcgc ggtacctttc tgcccaaggt ctgggaaagc ctgccggatc cacgagtttt
     2761 cctggacgag ctcaaggcca aagccggctt cccggctggc cactggtcgg atgccacaga
     2821 ggttttctac taccgaaccc agacgtttgc cgagccggtg gcagcgttcg gttccgtggt
     2881 gtcgcatggc ggtgattcgg tgtagattcc ccgggatggg ggaaaccaga tgaatcgctc
     2941 gctcaggggc cgggtcgccg tcgtcggcgt gggcgagacc atgtactaca aggccggcca
     3001 atcgccggat gccgagttca agctcgcgct caaggcgatc ctcgcggcct gcgaggatgc
     3061 aggcatcgac ccgaaggacg tcgatggttt cgcttcctac agcaacgacc gcagcgaccc
     3121 gtcgcggctg gccgcagcgc tgggttgtcg tgaactccgc tttgccaaca tgcagtgggg
     3181 cggtggcgga gggggcggct ccggggcgct cggcaacgcc gcggccgccg ttgccactgg
     3241 catggccgat tgcgtggtgg tttttcgcgc gctcgcccag gggcagttcg gccggttcgg
     3301 gcagggcccg cgacgcaacg tcatcgccgg tgacatggcc cacgtggtgc cgtacggcct
     3361 gatgtcgcca gctcagatgt tcgcgatgaa ggtgcagcga ttcatgcacg accacggtgt
     3421 tgagcagtcg gcgctgcggg cgatctcctt ggcgtcttac caccatgctc agaacaaccc
     3481 ccgtgcggtc atgcacggac ggcctctgga tgaggcgact tacgatgcgt ctcgatggat
     3541 cgtcgagccg tttcatctct acgattgctg tctcgagaac gacggcgcgg ctgccatgat
     3601 cgtggtgccg gcggatcgtg caggcgactg ccgcccggtt cctgcctatc tgcttggtgc
     3661 cgtgtccggt tcccactatc gggccggggc gtcggtgcac aacacgcccg actacgcgac
     3721 gagcacgttc aagacgctgg ctccccgcct gtacgagatg gccggggtgg tccccgccga
     3781 cgtcgacgtg ctgcagagct acgagaactt cactggcggc gtgctcatga gcatcgtcga
     3841 gcacggattc tgcgaggcgc aacggtgcaa cgagttcttc gtcccggagc ggttgacggc
     3901 gcccgatggg gaccttccgc tgaacaccag cggcggaaat ctcgccgagt gctacatgca
     3961 cggtctgggg ctcaacatcg aagcggttag gcagattcgc ggtgagtcca ccaatcaggt
     4021 gatgggcgca gagatatcca tggtgatctc gggccccatg gtcaccccgg tgagcgcgtg
     4081 catcctgggt tcggaggcgg cgctatgagc tactacctca gacccggcct gccacagccc
     4141 gcgccgcttc cggacggcct ggaccgacct tactgggacg ggctgcgcga ggatcggctg
     4201 atgctgcagc ggtgcgcgca gtgcggtggc tggcagtggg gtcccgagtg gatctgccac
     4261 cactgtctcg gctttgagct tgctttcgaa gcggtaacac cacgcggtat cgtctacagc
     4321 tacgaacggg tctggcaccc ggtccatccc gcgcttgccg agcagggccc ttacctggtc
     4381 gttctggtcg agattcccca ggcggacggg gtgcgattgg tcggcaacct gctcggcgac
     4441 ccgctccagg atgtggagat cggctcggcc gtggaggccg tgttcgagca tcacgaggac
     4501 gtggacgaac ccttcacgct gctgcagtgg aggcttctgg ccgactaatt ctgcccttga
     4561 actcggagtt cagcatgatc gaaatccacg gctactgtga cgagcgcttc tcgcccgtgc
     4621 gtgaggtctt tgcccgaaac tttgccgaca aggacgaggt tggggcttcg ctcgccctga
     4681 cggttgaggg cgagatggtc gtcgacatgt gggccggcca ccgggatgcc gcaaagtctc
     4741 tgccctggga gcaggacacc atcgtcaacg tgtggtcgac caccaagacc atgtcagcgc
     4801 tttgcgccct ggtgctcgcc gatcgcggcg agatcgatct ttacgccccg gtcacccggt
     4861 actggccggc gttcggccag aacggcaaag agaagatcga ggtaagacac ttcatgagcc
     4921 actccgccgg ccttgccggc accgatgagc cgattcgtgg tgaagctctc tacgactggg
     4981 agcgggtcgt caacgcgctt gaaaggcagg cgccctggtg ggaacccgga acggcgagcg
     5041 gataccacgc gatcactcag ggacacctca tcggcgaggt cgtgcgccgc gtgacgggta
     5101 tgtcctgcgg cacgttcttc cggcgcgaga tagctgagcc tctgggcgcc gactttcaca
     5161 tcggcaccgc tcccgaacac ttctcgcgca ttggcgacct gctgccgccg ggtgtgagcc
     5221 ttggcggggc tcttgcgcat gagccctcag ggagaggtgg aggcgagggc cgagcggcca
     5281 gcatcgcggt gcgcgcgttc cgcagcccgt tcgtgcgggc ggaagatgcg gcgaccgcgg
     5341 aatggcgcga agcggagatc ccggccgcca acggtcacgg caacgcgcga tccgtggccc
     5401 gcatccagac ggtgatcgcc aatggcggcg tgtctgctgg aactcggatc ctgtccgagg
     5461 ctgggtgccg ggcggttttc gacgagcaga cccacggccg cgacctcgtt ctcaaagcgc
     5521 cgctcacgtt cggtatggga tatgccctgt tacacggaca atctccaagg gaactcgcgt
     5581 tctggggcgg ctggggcggg tcctcggtgg tcgtcgctcc tcagtcgcgc atgtgcatcg
     5641 cttacgtgat gaatcgcatg gaaccgagcc ttctgggcga tgatcgtggg gtgtccttga
     5701 accgcgcggc gcttgccgtc atgggccgcg gctaggctca ctccttctcg gcttcaagac
     5761 gtgatagcgc ctcggccagc gcgtgcttgt gcgcgccctt ggccggcggg tagctgagcc
     5821 tgagctgctc gaggtgcgtg cagagcacct cggcgacgat gacgcgcatg gtccatttgt
     5881 cgtccgccgg aatcacgtac cagggcgcgt gatcgaagtg ggtggccgcg atggcggcct
     5941 cgtaggcttt catgtggtcg tcccagtgca cgcgctcgtc gatgtcgcgg gggtcgaact
     6001 tccagtgctt gtcgcggttc gtcaggcgct tcatcaggcg cgctttctgc tctttcttgg
     6061 aaacgttcag aaagaacttc aatacgatgg tgccgttggt gtgcaggtgc cgttccatgg
     6121 cacggatatc ctcgaagcgg ttctcccaga aatcgccgtt gacatgctcg ctgggcagat
     6181 tctgccgctc gatgatctcg gggtgaacgc gggcgactcc gacctcttcg tagtgcgaac
     6241 ggttgaagat gccgatgcgg cctcgctccg gcatggcgcg ccagtagcgc cacaggtagt
     6301 tgtggtcgag ttccttgtag gtgggctgct tgaagttgac cacttgaaag cccatgggat
     6361 tgacgccgct ggtgacgtac ttgatggtgc tgtccttccc cgctgcgtcc atgccctgga
     6421 agatcatgag aaccgaatag cggttgttgg cgtacagtcg ctcctgcagc tcggagagcc
     6481 gctcgacgtt cgcggtgacc agctcccttg cgtctggcag cactttccac agcgtggggt
     6541 gcgctgtcag cttggggtgc gctggatact cgaattt
//
LOCUS       DBNHLM010000327        23516 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_109870, whole genome shotgun sequence.
ACCESSION   DBNHLM010000327 DBNHLM010000000
VERSION     DBNHLM010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 23516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 23516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23516
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_109870"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..690)
                     /locus_tag="AC16QF_15045"
     CDS             complement(1..690)
                     /locus_tag="AC16QF_15045"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR6449926.1"
                     /translation="MTDLAAGNYRTLTFDCYGTLIDWENGILGYLQPLLESYDVHVLD
                     EWVLEFFAENEPALQAEGGSYRSVLARLLERLGTRCAFTPNDDALKGFADSIEYWQPF
                     ADTNAALRALSEHFDLAVVSNVDNELFSLSARQLGVEFAQVITADDVGAYKPARAMFD
                     EALRKVSGPVLHVAQSLYHDIAPAKALGLDTVWINRPSAAGSGATRFVEVEPTWTFGD
                     LSELVEAFDLD"
     gene            complement(683..1060)
                     /locus_tag="AC16QF_15050"
     CDS             complement(683..1060)
                     /locus_tag="AC16QF_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cereblon family protein"
                     /protein_id="HIR6449927.1"
                     /translation="MVDVLDKTRQDLDPKLKEIVEKDERNFVYCGICSHVLTREVERI
                     EVSGSHDHHCTNPFGLRFHIGCFAEALGCDISGEPMAADTWFPGFRWRHASCAECHQH
                     LGWYFEAGERYFYGLILDRIQHD"
     gene            1116..1763
                     /locus_tag="AC16QF_15055"
     CDS             1116..1763
                     /locus_tag="AC16QF_15055"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR6449928.1"
                     /translation="MHRAVERKGGRAALDALLPAARSGSDLAALPDRDYLAEMTRCVF
                     RSGFVWKIIENKWPGFEVAFRGFDVAGCAMLSDEDIEQLSADERIVRHAAKIRSVPAN
                     AAFILDVRREAGGFGRYLADWPEDEIVGLWDELKRRGSRLGGQTGRYFLRFVGKDTPI
                     LSRDVVKALIGQGVIDREPTSKKALARVQDAFNEWHAESGRELCQISRVLSLSVD"
     gene            complement(1968..3173)
                     /locus_tag="AC16QF_15060"
     CDS             complement(1968..3173)
                     /locus_tag="AC16QF_15060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6449929.1"
                     /translation="MTGMVIVGAGHAAGQAAASLRQAGYEGEITIIGDEPHIPYQRPP
                     LSKQYLAGEQGLERVYLRPEKFYADKEITLRMGVSVTAIDTQAHTVTTDDGETIGYEK
                     LLLATGSRPRILNVPGSDLAGIHYLRTIADVDGIRAELAEGRRLVIVGGGYIGLEVAS
                     VGIQAGLEVHVLEMEERILQRVTTPEMSDYYHQLHTGRGVHIHTSTMVSGFTGEGRVG
                     AVQCGDEEIAADIVVVGIGIIPNVEVAQAAGIECDNGIVVDEGCRTSADDVFAAGDCT
                     NHPNPLLHRRLRLESVPNAMEQARVAAANMCGADKVYESIPWFWSDQYELKLQMVGFS
                     ADGDTQVVRGDKAANQFAVFYLKDGAVVAADAVNSPKEFMVCKQLVGKPVDAARLGDP
                     DVELKSLLA"
     gene            3345..4415
                     /locus_tag="AC16QF_15065"
     CDS             3345..4415
                     /locus_tag="AC16QF_15065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HIR6449930.1"
                     /translation="MRYRTLHEIVRQAQRNLDRDCRDYLLGGADTESSLRRNRHGLDS
                     WVFRPRILNDVSEVNLTIELLGAPMRIPVVLPPIGSVQLFEPGGGQTVAEAAHEFGIL
                     QILSSVCTPDFEAVAAAVPGPRIYQLYLMGDRVWLDDMIGRTIEAGYTGFCLTADTQI
                     YSRRERDILKGFVPPSGSQAGSRDFNYQARMTWETVAHIKSTFDIPLIVKGVNAGEDA
                     ARCVEAGVDVVYVSNHGGRQLDHTRACIDALPEVVEAVAGRVPVIVDGGFLRGADVVK
                     GLCLGADVVGMGRLEGLAMAAGGRGALVRALEIVEQEIRTTMALLGVSDLADLNPGLM
                     ERAQPLAESTVFSAFPLLEEGY"
     gene            4516..6567
                     /locus_tag="AC16QF_15070"
     CDS             4516..6567
                     /locus_tag="AC16QF_15070"
                     /inference="COORDINATES: protein motif:HMM:NF024144.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HIR6449931.1"
                     /translation="MAPTLRTSIRWFGTFLIVFTMAACGGGGGGDDAPRPPGTDASLS
                     GMSLSSGSLEPAFAAATTEYTADVPFAVAEITLTATTSDSRASLTVDGATASSGSPSD
                     PVALAVGETKVDVVVTAEDGTTTRAYSITLTRAAAATNADLVSLSLSAGALDQIFDTT
                     QPDYTATLGYLAASVLVLAEPEDPNAVVTVSAGTVDERVALDEGVNVIDLLVTAEDGT
                     TTRAFELTITRGAALDFAQRLYVKPDVIRESYRFGAAVDLEGSWLAVGAPGDQSLANT
                     IDGDDGDFAGVNVGGVTVFQADASGLWTRQAYLKAANAGDGDGFGEALAMSGNTLAVG
                     APLEQGLDGAPDDDSGNGVGAVYVFVRDESDVWTQQAYLKASNADNGDEFGRYLDLDG
                     DTLVVGAWHEQSAGAEDDNSLADAGAAYVFVRDESGEWSQTAYLKPDEIDTGDLFGGN
                     VALSGDTLVVGATGEDGVAGDPADNSSNNSGAAYVFERDLAGGWSQSAYLKADNADPD
                     DAFGANPDLEGDVLMVAAIGEQSALPGDPDDNSGNNVGAVYEFARDIDGNWSQTGYFK
                     ASNAELGDEFGSAVAYEGDTLVVGARKEAGLSGNENDNSGARVGAAYVFFREDGIWRQ
                     AAYVKASNPDNEDEFGNAVSFDGNALVVTAEGEDSAETGLGGAGLDNSLNAAGAGYLF
                     R"
     gene            complement(6556..8124)
                     /locus_tag="AC16QF_15075"
     CDS             complement(6556..8124)
                     /locus_tag="AC16QF_15075"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6449932.1"
                     /translation="MVSGLHPLHWAEVEPDKTAWIFGDQRVSYARLVQSALKGAQLLR
                     KLGLKTGDGVAVVAENHPNTLAFFWAAQMAGLYYTPISVQFHPDEIGYILADCDARLL
                     MASRAQEERVAGAPQPMRLTLDTWLDAIAGEPATLIADACEGAEMLYSSGTTGRPKGV
                     RAASPGAPLGTVSELFRRRLALHEVDGQAVYLSTAPLYHSAPLRYNTMMHRCGATSVI
                     MPRFDAAESLALIETHRVTHSQWVPTMFVRLLKLPAETREAFDLSSHRLAVHAAAPCP
                     VPIKRQMLDWWGPILHEYYSGTEGNGQTAITPEEWLAHPGSVGKPVLGNVHILDEAGN
                     EAPIGTEGCVYFGDGARFEYYKDPAKTAASYDERGWSTLGDIGYLDQEGYLYLTDRAS
                     HMIITGGVNVYPREVEDVLVTHPAVQDAAVFGIPDPEFGEQVKAAIELVPGTTVNGDE
                     LIAFCRARIAHLKCPKSVDFHDTLPRHQTGKLYKEALKRPYWPEPSANKEPSANKEPS
                     AKKEPSAKKEPSAE"
     gene            complement(8118..8453)
                     /locus_tag="AC16QF_15080"
     CDS             complement(8118..8453)
                     /locus_tag="AC16QF_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003249769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HIR6449933.1"
                     /translation="MSDDQKHIYKVLFHNQGQIYEVYAHNIYQSDLYGFIEVEDYIFG
                     KKTQVVIDPAEDKLRTEFDGVQRSFIPMHAVIRIDEVEREGVAKVTESSGGNITPFPV
                     PMPKDTGKS"
     gene            8546..9877
                     /gene="miaB"
                     /locus_tag="AC16QF_15085"
     CDS             8546..9877
                     /gene="miaB"
                     /locus_tag="AC16QF_15085"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR6449934.1"
                     /translation="MMKKLYIKTHGCQMNEYDSARMADLLRESQGYELVAEPEDADLI
                     LLNTCSIREKAQEKVFHQLGRWRALKVRKPGLVIGVGGCVASQEGAAIQSRAPHVDVV
                     FGPQTLHRLPEMIDAAAGPARVPLVDVSFPEIEKFDRLPQPRAEGPSAFVSVMEGCSK
                     YCSFCVVPYTRGEEVSRPVADVMREVIQLAGQGVREVNLLGQNVNSYLGPIDDDHADL
                     AELIYYVAEVDGIDRIRFTTSHPLDFNDSLVRAFADVPELVDHLHLPVQSGSDRVLAL
                     MKRGHTVSEYREKIAALKEVRGGISLSSDFIVGFPGETEADFQATMDLVAEIGFDTSF
                     SFVYSARPGTPAAALPDDTPEAVKRERLALLQGLLRSQADAIACDMVGTRQTVLVTGE
                     SRKNREELQARTENNRVVNFPCPDRRLVGGFVDVDITEALPNSLRGVMAIT"
     gene            9970..11007
                     /locus_tag="AC16QF_15090"
     CDS             9970..11007
                     /locus_tag="AC16QF_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR6449935.1"
                     /translation="MSEDDSRVVALTTNVSLEPNEPRRLAALCGPFDQNLKHLERRLG
                     VHIRSRGNLFQISGPEQRVQAAGALLSQLYRETLEHETIDPDMVHLFLQESGVDELLA
                     RQRGPDEMPADGEGAVISTGRKSVKPRGGNQTRYVERIRGHDVNFGIGPAGTGKTYLA
                     VACAVQALESQQVSRILLVRPAVEAGEKLGFLPGDLAQKIDPYLRPLYDALYEMMGVD
                     RVNKAIERNIIEVAPLAYMRGRTLNNAFIILDESQNTTIAQMKMFLTRIGFGSTAVIT
                     GDITQIDLPKGQKSGLVNVRNVLSGIDGISFTHFGSKDVVRHPLVQKIVDAYEAYQEP
                     HASPDSDRDAG"
     gene            10997..11452
                     /gene="ybeY"
                     /locus_tag="AC16QF_15095"
     CDS             10997..11452
                     /gene="ybeY"
                     /locus_tag="AC16QF_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017903146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR6449936.1"
                     /translation="MPVEVQIASVQAGLPSVARIEGWASAALDELGDEGEPPELCIRV
                     VDGEESRELNARFRGHDKPTNVLSFPADVDLPEAKLLGDVVICAPVVGVEAAEQGKSP
                     EDHFAHMVVHGVLHLSGYDHEQAGEAELMEAIERRILERVGIADPYAER"
     gene            11459..12294
                     /locus_tag="AC16QF_15100"
                     /pseudo
     CDS             11459..12294
                     /locus_tag="AC16QF_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798742.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
     gene            12291..13772
                     /gene="lnt"
                     /locus_tag="AC16QF_15105"
     CDS             12291..13772
                     /gene="lnt"
                     /locus_tag="AC16QF_15105"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIR6449937.1"
                     /translation="MKPLLALACGALVPLSLAPFGWWPLGLVAVGGWFALLQSSDRGG
                     LLLGWLFGLGKYAAGVSWIYVSINVYGNAPPPLAAFLVALFAGGLALFPMVNAWVFQR
                     LRTKGAPLVNAWLFACLFVAFEWLLTWFLTGFPWLYPAYGHVETVLGGLAPAGGVMLV
                     SLGLAGSACSVAAAHQCARRLPVLTAALAPWVVGAVLGAVSWVEPGAVRTVALVQGNV
                     DQAVKWLPENRRPIIDRYRGLSEDHWGVDVMVWPEAAITVMAHDAGEVLGDLEREGER
                     SGTAVVLGLPAVERLADDRIVLRNTAIALGDGSGRYVKRRLVPFGEYVPLEGWLRGLI
                     EFFDLPMSRAAPGDWRQPLLRVGQEQAALAICYEIVYPDLVRAQAVGADVLVTISNDT
                     WFGASIGPLQHLQMARMRALENGRWLLRGTNNGVTAIVDHRGRLVARLPQFEAGVLRG
                     EYRLVAGRTPYNRHGDGPVLVLVAFGLGVAFWRRLGAARGADG"
     gene            13788..14318
                     /locus_tag="AC16QF_15110"
     CDS             13788..14318
                     /locus_tag="AC16QF_15110"
                     /inference="COORDINATES: protein motif:HMM:NF016290.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly lipoprotein LptE"
                     /protein_id="HIR6449938.1"
                     /translation="MRTRLRYTWLIPPLFLLIGGCGFQLRTWDLGTSVESFYVRDASN
                     NPLASPLRRALRQAGVDEAGNASDAQVVVLLLNAQRGRRSVSVTGQARAAEYEISLGV
                     QYGVLDGQGQELIAPRWVRGTRVFRVDRDNIVGTSEEQALLEREIQNDLIQQIVRALN
                     TVSGPAGPAGQTANAA"
     gene            14308..15312
                     /gene="holA"
                     /locus_tag="AC16QF_15115"
     CDS             14308..15312
                     /gene="holA"
                     /locus_tag="AC16QF_15115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR6449939.1"
                     /translation="MPLRLSDLQSRLAAGLAPVYFISGDETLLVQEACDAILGKAHSE
                     GFTERSVFHVEKGFRWNDILQDAASLSLFAERRIIDVRVPGNTFDREASEVLRTYSAE
                     PSPDTLLLIRSARLEPRQRSSAWFKALDAAGAVVLVWPVGIAELPRWLAGRLRAAGLS
                     LTPDAQSYLAQQVEGNLLAAVQEVEKLRLADLPQPIGSEELMRQMEDSAHYDSFELID
                     AVFAGDGARVSRILANLRQEGVSLYAVLAALTGQLRRLPGGRMAPQRQRLVDGFMRRM
                     RSPRNLDRALAQCALVDAQGKGQLLGDAWLSLENVCLRLAGQRGLPSLEEELPSLRRA
                     "
     gene            complement(15351..16544)
                     /locus_tag="AC16QF_15120"
     CDS             complement(15351..16544)
                     /locus_tag="AC16QF_15120"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6449940.1"
                     /translation="MAELEVLTHDEEMMSLARRALEHFVNKTTDQAASTLEMPVEAYV
                     GQSRYQAERDRIFMHLPLALALSLELPEPGCYRAMTVMNVPVLLIRGEDDTVRAFVNV
                     CTHRGARLCPEGAGERRVISCPYHAWTFDFEGRLSGRYAAETFGDLDPEAYALTELAC
                     AERAGVIWVILTPGEQFDADAWLGGFAEQLGSLHLTDWYLYEQRDLEGPGWKVAMDGY
                     LEIYHHNPLHGATVGQHTVGNLLVLDTYGPHQRLTLGRRTLGTLAETPESEWAPLEHI
                     RLIHSGFPNLSVSGILGDHCLVSQIFPGEDPFGTVTRQSILVAEKPATDAAEAAARNF
                     SDMVLQAVRDEDYAIGQTIQAGLRAPARAAFIFGRNEPAVQNYHRWVANFMTQTAEAR
                     DWGGV"
     gene            complement(16531..17094)
                     /locus_tag="AC16QF_15125"
     CDS             complement(16531..17094)
                     /locus_tag="AC16QF_15125"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6449941.1"
                     /translation="MSELDELRTRVDRLESREAIRELVSAYAVACDHHDMPALDDLFT
                     EDAVFASPNGAMIATGRNAIHDMFVRILGIRGPGYHWTHDVTVNIDPSDPDRATGVVH
                     SHAETTPSGVMSIAAMRYHDSYRREAGIWRFASREISFLYYVPVSEYGASLTRPDRVV
                     MGTERHAADYPEALEAWQAFEASHGGA"
     gene            17403..18404
                     /gene="cobS"
                     /locus_tag="AC16QF_15130"
     CDS             17403..18404
                     /gene="cobS"
                     /locus_tag="AC16QF_15130"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HIR6449942.1"
                     /translation="MTVNQSGLSADMAPDTTVSAREQFGVEHDFQVPAFSKRSDYVPE
                     VDVDYRFDPDTTLAILAGFAHNRRVMIAGYHGTGKSTHIEQVAARLNWPCIRVNLDSH
                     ISRIDLIGKDAIVLEEGKQVTEFREGILPWAIQHPVALVFDEYDAGRPDVMFVIQRVL
                     EVEGKLTLLDQNKVITPHPFFRLFSTTNTIGLGDTTGLYHGTQQINQAQMDRWSIVTN
                     LNYLEHDAEVDIVLGKAKHYAETEDGARTISNMVAVADLTRKGFVNGDVSIVMSPRTV
                     ITWAENAEIFGDIGYAFRVTFLNKCDELEQPIVAEYYQRCMGEDLGYATAGIALKSA"
     gene            18421..20232
                     /gene="cobT"
                     /locus_tag="AC16QF_15135"
     CDS             18421..20232
                     /gene="cobT"
                     /locus_tag="AC16QF_15135"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HIR6449943.1"
                     /translation="MSEEEHPLEPFKRATTATIRAIAENDELEVTFGQGPPTVRGNRI
                     RVPLPAVGSAPQEVDAIRGMGDEFALRIRYHDDDVHRSRAPRSGPAQEMFQWIEDARI
                     ASIGSLRMEGVAQNLDASLEVQCQQAAFDTITAEADAPLSVAVGLIVRQHLTGRDLPP
                     SAENVVRFWRAYVEENAGDDIDALRDCLLDQAEFAERCRAIIADLGLGAELDEPPELD
                     ESQDDTDAMDEDQDADSDISPDDVVLDDDSMMDESAEGDATMMEMDADMDMDELGAEM
                     EPDEAPTQMPDESGRIRMDFNYEAYTQEFDETVRAEELCDADEMIRLRALLDQQLVPL
                     QHATSKLANRLQRKLMAKQSRTWEFDLDEGMLDASRLSQVIIDPMNPLAYKQEKEMQF
                     RDTVVTMLIDSSGSMRGRSITIAAMCADILGRTLERCSVRVEVLGFTTRAWRGGQSRE
                     KWIASGKPSNPGRLNDLRHIIYKAADDPWQRTRRNLGLMMREELLKENIDGEALIWAH
                     NRLVVRHEERKVLMVISDGLPVDNSTLLVNPSNYLEQHLKYAIDQIENYSSVELIAIG
                     IGHDVTHHYQRAVTITDAEQLGGAMTEQLAELFEAKR"
     gene            complement(20291..22780)
                     /gene="leuS"
                     /locus_tag="AC16QF_15140"
     CDS             complement(20291..22780)
                     /gene="leuS"
                     /locus_tag="AC16QF_15140"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR6449944.1"
                     /translation="MSSEAENQYAPGEVEAKWQRLWEERGTNSFTADELRSAQSPYYN
                     LMMFPYPSAEGLHVGNMYAFTGADVHGRFHRLTGRDVFEPIGFDAFGIHSENFALKQG
                     VHPMDLIPSNIANFTRQLKAIGGMYDWDHTVDTTDPAYYKWTQWLFLQLFKAGLAERK
                     EAPVNWCPSCMTVLANEQVIQGLCERCDSAVEQRHIAQWFFRITEYAQRLLDNLEHID
                     WSESTRKAQQNWIGRSEGATLRFPVIEGGNGDPGTVDPLTVTADGDVIEVFTTRPDTL
                     FGATFMVLSPEHPLVASITTDAQQAAVAAYQAQAAKTDLVSRQKADKEKTGVFTGGYC
                     RNPANGAPIPVWIADYVLMEYGTGAIMAVPGGDERDFEFATAMGIEIVRVVAGEGDGA
                     DTPLAEAYTGDGVLVNSGPFDGLGVADGKRAVTAWLGERGLGELKVNYRLRDWCISRQ
                     RYWGPPIPIVHCDACGAVAVPEDQLPVELPRVDDFKPDDTGVSPLARVASWYETTCPG
                     CDGPARRETDVSDTFLDSSWYFLRYPSTDRNDAPMDDGITARWLPVDSYIGGNEHAVL
                     HLMYSRFINMVLKDLGHIDFEEPFVKFRAHGLITRNGAKMSKSRGNVITPDAIIDQYG
                     ADTFRTYLMFMGPLEEGGDYRDEGIQGPFGFLHRLWDAVVTAKPDTAADEDVERKLHK
                     TIKQATEQIPELGYNTAIAAMMECLNAVRAGGRQPAVAEVEPLVVMIAPFAPHLAEEL
                     WERLGHGKSIFAGANWPAYDEAKVRDEVVSVAVQVNGKLRGTISLAPGTDQAEAEALA
                     RAEENVARHLDNAEVRRVIYVPNRLLNFVVG"
     gene            complement(22803..23087)
                     /locus_tag="AC16QF_15145"
     CDS             complement(22803..23087)
                     /locus_tag="AC16QF_15145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6449945.1"
                     /translation="MIAVIAKLNVTPGREADFEKAMLALAAQVRENEPGNQLYTLCRD
                     ADGNYLMMELYDSEEALAAHGQTEHIKASGASFAGLMSGRPEIQRLQVIG"
     gene            23139..>23516
                     /locus_tag="AC16QF_15150"
     CDS             23139..>23516
                     /locus_tag="AC16QF_15150"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6449946.1"
                     /translation="MHRRGLIGLLEDYGRRHPAESAVVERFLAFVREHERCFERDCFP
                     GHVTGSAWLVNGAGTHVLLTHHKKLGRWLQLGGHADGETDPLLAATREAEEESGLAVE
                     PVGEAVFDVDIHEIPARGPDPLHL"
BASE COUNT         3905 a         7517 c         7938 g         4156 t
ORIGIN      
        1 tcagtcgagg tcgaaggcct cgacgagctc gctgagatcg ccgaacgtcc aggtcggttc
       61 cacctcgacg aaacgcgtgg ccccgctgcc ggccgcgctc ggccggttga tccacacggt
      121 gtccagaccc agcgctttgg cgggcgcgat gtcgtggtag aggctctggg cgacgtgaag
      181 cacgggcccc gatacctttc tgagcgcctc gtcgaacatc gcgcgggcgg gtttgtaggc
      241 cccgacgtca tcggcagtga tcacctgcgc gaactccacg cccagctgcc tggcggagag
      301 ggagaacagc tcgttgtcga cattcgagac cactgccagg tcgaagtgct cggacagggc
      361 gcgcaacgcg gcgttggtgt ctgcgaacgg ctgccagtac tcgatgctgt ctgcaaagcc
      421 cttcagggcg tcatcgttgg gcgtgaacgc gcaccgcgta ccgagccgct ccagcagacg
      481 cgcaagcacc gatcgatacg agccgccctc ggcctgcagc gcgggttcgt tctccgcaaa
      541 gaattccagc acccactcgt cgagcacgtg cacgtcgtag ctctcgagca ggggctgcag
      601 gtagccgagg atgccgttct cccaatcgat gagcgtcccg tagcagtcga aggtcagcgt
      661 gcggtagttg ccggcagcga ggtcagtcat gctgaatgcg gtccagtatc aatccgtaga
      721 agtatctttc gcctgcttcg aaataccagc ccagatgctg gtgacactcc gcgcaggatg
      781 cgtggcgcca gcggaagccc ggaaaccagg tgtctgccgc catgggctct cccgagatgt
      841 cgcagcccag ggcctcggcg aagcagccga tatgaaagcg aagaccgaac gggttcgtgc
      901 aatgatgatc gtgactgccc gacacctcga tgcgctcgac ctcccgggtc agcacgtgcg
      961 agcagatccc gcagtagacg aaattccgct cgtccttctc gacgatctcc ttgagcttcg
     1021 ggtctaggtc ttgacgggtt ttgtcgagga catccaccat ggtgttcgtc gtggctcttt
     1081 gggttccgcg ggatatggaa tcgttcgata gcatcttgca cagggcggtt gagcgcaagg
     1141 ggggcagggc ggccctagac gcgctgctgc ccgcagccag gtcaggcagc gatctggccg
     1201 ccttgccgga ccgggactac ctcgccgaga tgaccaggtg tgtcttccgc tcgggtttcg
     1261 tgtggaaaat catcgagaac aagtggccgg ggttcgaggt tgcattccgc gggttcgatg
     1321 tggccggctg tgcgatgctg tccgatgaag acatcgagca gctctcggcg gacgagcgga
     1381 tcgtgcgtca cgcagcgaag atccgttcgg ttcctgccaa cgcggccttc attctggacg
     1441 tgcgccgtga agccggtggc ttcgggcgct acctggccga ctggccggaa gacgagatcg
     1501 tcggtttgtg ggacgagctc aagcgtcgcg gctcgcggct cgggggccag accggtcgtt
     1561 actttctgcg tttcgtcggc aaagacacgc cgatcctgtc cagagacgtc gtcaaagccc
     1621 tgataggtca gggcgtgatc gatcgggagc ccaccagcaa gaaagcgctc gcgcgggtcc
     1681 aggacgcctt caacgaatgg cacgcggaga gcgggcgcga gctgtgccag atttcccggg
     1741 tcctttccct gtccgtggac tgagcggccc cgggcgaagt gggcccataa agaaacgccg
     1801 gatccagagg atccggcgtg aaaagacgcg cgggaagggg agaggaggag acgatcccgc
     1861 gcacctgtcg cagaaatcat tgtcatcttt ccctgtgccc atacccatca gggattctac
     1921 gtatggcacg gacggcgaag gtgtcgatcg gccgcctttg aggctgctca agccagcagg
     1981 gacttcagct ccacatccgg atcgcccagc ctggccgcat ccaccggctt gcccacgagc
     2041 tgcttgcaca ccatgaactc tttcgggctg ttcaccgcgt cggcggccac caccgcgccg
     2101 tctttgaggt agaacacggc aaactggttg gctgccttgt cgccccggac cacctgcgtg
     2161 tcgccgtcgg cggagaagcc gaccatctgg agtttcagct cgtactggtc cgaccagaac
     2221 catggaatcg actcgtacac cttgtctgcg ccgcacatgt tcgcggccgc tacccgggcc
     2281 tgctccatgg cattgggcac ggattccagg cgcaggcgcc ggtggagcag cgggttggga
     2341 tgattggtgc agtcgccggc ggcgaacacg tcgtccgccg acgtgcggca gccttcgtcc
     2401 acgacgatgc cgttgtcgca ctcgatcccg gcggcctggg caacctcgac gttggggatg
     2461 atgccgatgc ccacgaccac gatgtcggcg gcaatctcct cgtcgccgca ctgcactgcg
     2521 ccgacgcggc cctcgccggt gaagccgctc accatggtgg aggtatggat gtgcaccccc
     2581 cggccggtgt gcagctggtg gtaatagtca gacatctcgg gcgttgtgac cctctgcagg
     2641 atgcgctctt ccatctccag cacgtggacc tccaggcccg cctggattcc cactgacgcc
     2701 acctcgagcc cgatatagcc gcccccgacg atgacgagcc ggcgcccttc agccagctcc
     2761 gcgcgaatgc cgtccacgtc ggcaattgtc cgcaggtagt gaatgcccgc aaggtcgctg
     2821 ccgggtacgt tgagaatcct gggccgactg cccgtggcga gcaggagctt ctcgtagccg
     2881 atggtttcgc cgtcgtccgt ggtcacggtg tgtgcctggg tgtctatcgc ggtgacgctg
     2941 acgcccatgc gcagggtaat ttccttgtcc gcgtagaact tttcggggcg caggtacacc
     3001 ctttccaggc cctgctctcc ggccaggtac tgcttggaca ggggtggccg ctggtagggt
     3061 atgtgcggct cgtccccgat gatggtgatc tcaccttcgt aacccgcctg gcgcaggctg
     3121 gcagccgcct gccccgcagc atgtccggcc cccacgataa ccatccccgt catttgctca
     3181 gctccttggt tcgtccgctc gccccggccc gtgcgccggc ccgcgctgga ggggcatctt
     3241 actaaggttc ccttgctcag gacgagccga aaccgggatt gtcgatggat ttgtccggcg
     3301 caggcgttag catccgcggt cgagcatcga aggtggaaat cgcgatgagg tatcggacgc
     3361 tgcatgagat cgtgaggcag gcccagcgca atctggatcg cgactgccgg gactacctcc
     3421 tcggcggcgc ggacaccgag tcttcgctgc ggcgcaaccg ccatgggctc gactcctggg
     3481 tctttcgtcc gcgtatcctg aacgacgtca gcgaggtgaa tctgaccatc gagctgctgg
     3541 gcgcgccgat gcgcatcccg gtggttcttc cgcccatcgg ttcggtgcag ctgttcgagc
     3601 cggggggcgg ccagacggtt gccgaggcgg cgcacgagtt cggcatcctc cagatcttga
     3661 gctccgtgtg cacgcccgat ttcgaggccg tggccgccgc ggtgcctgga cccaggatct
     3721 accagctcta cctgatgggc gatcgggtct ggctggacga catgatcggc cggaccatcg
     3781 aagccgggta cacgggcttc tgcctgacgg ccgacaccca gatctactcc aggcgcgagc
     3841 gggacattct caagggcttc gtgcccccgt cgggcagcca ggccggcagc cgggacttca
     3901 actaccaggc gcgaatgacc tgggagacgg tggcgcacat caagagcacg ttcgacatac
     3961 cgctcatcgt caagggcgtc aacgcgggcg aggacgcggc gcgctgcgtg gaggcgggtg
     4021 tggacgtcgt ttacgtttcg aaccacggtg gtcgtcagct ggaccacacc agggcctgca
     4081 tcgacgcgct gccggaggtc gtcgaggccg tggccggccg cgtgccggta atcgtcgacg
     4141 gcggttttct gcgtggtgcc gatgtggtca agggcctgtg cctgggggcc gacgtggtcg
     4201 ggatgggtcg tctcgagggc ctcgccatgg cggccggagg acgtggggcc ctcgttcggg
     4261 cgctggagat cgtggagcag gaaatccgga ccaccatggc gcttctgggc gtctccgatc
     4321 ttgccgatct caatccgggc ctcatggagc gggctcagcc tctggcggag tccacggttt
     4381 tcagcgcgtt cccgctgctg gaagaggggt attgagcccc tgcaaccaag ggaagtgacc
     4441 tagtttcaaa tacgtcaaga tatgttacgg ttacaccgtc gccacttggg cagtttattt
     4501 gcggaaggtc cggtcatggc acctacattg cgaacgtcta tccgctggtt tggaacgttt
     4561 ctcatcgtct tcaccatggc ggcatgcggt ggcggaggag gcggggacga tgcgccacgc
     4621 ccgccgggca cggatgccag cctgtccggg atgagcctgt cgtcgggcag cctggaaccg
     4681 gcgtttgcag cggctacgac ggaatacacg gccgacgtgc ccttcgcggt ggccgagatc
     4741 acgctgacgg cgacgaccag cgactcccgc gcgtctctca cggtagacgg tgcgaccgcc
     4801 tcatccggaa gccccagcga cccggtcgcg cttgccgtcg gggaaacaaa agtggacgtc
     4861 gtcgtcacgg ccgaggacgg aacgacgacc cgtgcttatt cgatcactct cacccgggcg
     4921 gccgccgcga ccaatgccga cctggtctcc ctctccttga gcgccggcgc tctggatcag
     4981 atcttcgaca cgacccagcc ggactacacg gccaccctcg ggtacctggc cgcatcggtg
     5041 ctggtgctgg cagagcccga ggatccgaac gccgtggtca ccgtcagcgc cgggaccgtt
     5101 gacgaacggg ttgcgctcga tgagggcgtg aacgtgatcg acttgctggt gacggcggag
     5161 gacggcacca ccacccgcgc cttcgagctg acgatcaccc ggggcgctgc gttggatttc
     5221 gcgcagcggc tgtacgtcaa acccgacgtc atccgcgaga gttaccgttt cggcgcggcc
     5281 gtggacctgg aaggttcctg gcttgcggtc ggcgcgcccg gagatcagag ccttgccaac
     5341 accatcgacg gggacgatgg cgacttcgcc ggcgtgaacg tgggcggggt caccgtcttc
     5401 caggcagacg cctcgggcct ctggacccgc caggcctacc tcaaggccgc caatgcaggg
     5461 gatggtgacg gcttcgggga ggctctcgcc atgtccggca acacccttgc agtgggggcc
     5521 cctctcgagc agggcctgga cggcgccccg gatgacgaca gcggaaacgg ggtgggcgcg
     5581 gtgtacgttt tcgtgcgcga cgaaagcgac gtctggaccc agcaggccta cctcaaggcg
     5641 agcaacgccg acaacgggga tgagtttggc cgttacctcg acctcgacgg cgacaccctg
     5701 gtggtgggcg catggcacga gcagagcgcg ggagccgagg acgacaactc cctggccgac
     5761 gcaggcgcgg cctacgtgtt cgtccgcgac gagtccggcg agtggtctca gaccgcttac
     5821 ctgaagcccg acgagatcga cacgggcgat ctcttcggtg gcaacgtcgc gctttccggc
     5881 gacacgctgg tggtgggggc taccggcgag gacggggtgg ccggcgaccc ggccgacaac
     5941 agctcgaaca actccggcgc cgcctacgtg ttcgaacgcg acctggcggg gggctggtcc
     6001 cagagcgcat acctcaaggc tgacaacgcg gacccggacg acgccttcgg cgcgaacccg
     6061 gacctcgagg gcgacgtgct catggtggcg gccatcggcg agcagagcgc cctgcccggg
     6121 gacccggacg acaactcggg caacaacgtg ggggccgtct acgaattcgc tcgagacatc
     6181 gacgggaact ggagccagac gggctacttc aaggcgtcca acgcggagct tggcgacgag
     6241 ttcggctcgg ccgtggccta cgagggcgac accctggtgg tgggcgcccg caaggaggcc
     6301 ggtttgagcg gcaacgagaa cgacaattcg ggggcgcgtg tgggggccgc ctacgttttc
     6361 ttccgtgagg acggcatctg gcgccaggcc gcgtacgtga aagcgtccaa ccctgataac
     6421 gaggacgagt tcggcaatgc tgtgagcttt gacggcaacg ctctggtggt caccgcggaa
     6481 ggcgaggaca gcgcggagac cgggctcggc ggagcgggtc tcgacaactc cctgaacgcc
     6541 gcgggcgccg gctacctatt ccgctgaagg ctctttcttc gctgaaggct ctttcttcgc
     6601 tgaaggctct ttattcgctg aaggctcttt attcgctgaa ggctccggcc agtagggccg
     6661 cttgagggct tctttgtaga gcttgcccgt ctggtggcgc ggcagggtgt catggaagtc
     6721 caccgacttc gggcacttca ggtgggcgat gcgcgcccgg cagaaggcga tgagctcgtc
     6781 gccgttcact gtggtgccgg ggacgagctc gatggcggct ttgacctgct cgccgaattc
     6841 cgggtccgga atgccgaaca cggccgcgtc ctgcaccgcc ggatgggtca ccagcacgtc
     6901 ctccacctcc ctcggataga cgtttacccc gccggttatg atcatgtgcg aggcgcgatc
     6961 ggtgaggtac aggtagcctt cctggtccag atagccgatg tcacccagag tggaccatcc
     7021 gcgctcgtcg taggaggccg ccgtcttcgc cggatccttg taatactcga aacgcgctcc
     7081 gtcgccgaag taaacgcatc cctcggtgcc gatcggcgct tcgttgccag cctcgtccag
     7141 gatgtgcacg ttgccgagga cgggcttgcc cacggacccc gggtgggcga gccactcttc
     7201 gggcgtgatg gccgtctgcc cgttgccctc cgtgcccgag tagtactcgt ggaggatggg
     7261 gccccaccag tcgagcatct gccgcttgat gggcacgggg cacggtgccg cggcatggac
     7321 ggccaggcgg tggctggaaa ggtcgaacgc ttcccgggtc tctgccggca gcttcagcag
     7381 ccgtacgaac atggtgggca cccactggct gtgggtgacc cggtgagtct cgatcagcgc
     7441 caggctttcg gcagcgtcga agcgcggcat gatcacgctg gtggcgccgc agcggtgcat
     7501 catggtgttg tagcgcagcg gcgcggagtg atacagcggc gcggtggaga ggtagacggc
     7561 ctggccgtcc acctcgtgca gcgccagccg gcgcctgaac agctcggaga ccgtgccgag
     7621 aggggcgccc ggacttgccg cgcgcacgcc tttgggccgg cccgtggtgc cggaggagta
     7681 gagcatctcc gcgccctcgc aggcgtcggc gatcagggtc gcgggctcgc cggcaatggc
     7741 gtcgagccag gtgtccagag tcaggcgcat cggctggggc gcgccggcca cccgctcctc
     7801 ctgggcgcgg ctcgccatca gcagccgggc gtcgcagtca gcgaggatgt agccgatctc
     7861 gtcgggatga aactggacgc tgatgggggt gtagtagagc cccgccatct gtgccgccca
     7921 gaagaatgcc agggtgttcg ggtggttctc cgcgaccacc gcgacgccgt cgcccgtttt
     7981 caggccgagc ttgcgcagga gctgcgcgcc cttcagcgcg ctttggacca gccgcgcgta
     8041 gctcacgcgc tgatcgccga agatccatgc ggtcttgtcg ggctcgacct ccgcccagtg
     8101 gagcgggtga aggccgctta cgatttgccg gtgtccttgg gcatgggaac cgggaagggg
     8161 gtgatgttgc cgccgctgga ctccgtgact ttcgccacgc cctcgcgttc gacctcgtcg
     8221 atgcggatca cggcatgcat ggggatgaag ctgcgctgca cgccgtcgaa ctccgtgcgc
     8281 agcttgtcct cggccgggtc gatgaccacc tgggtcttct tgccgaagat gtagtcctcc
     8341 acctcgatga agccgtagag gtcgctctga tagatgttgt gcgcgtagac ctcgtagatc
     8401 tgaccctgat tgtgaaacag cactttgtag atgtgcttct ggtcgtcgct cattggcgtt
     8461 gatacctgct gtgccagatg tcctatatag aggtgtatgt ggaaaattca agcatttata
     8521 atcccgcgcc ccgaatccgg gtcgtttgat gaagaagctc tacataaaaa cccacggttg
     8581 ccagatgaac gagtacgact cggcccgcat ggccgatctg ctgcgggaga gccagggtta
     8641 cgagctcgtc gccgagccgg aagacgcgga cctgatcctg ctgaacacct gctcgatccg
     8701 cgagaaagcc caggagaaag tctttcacca gctgggtcgg tggcgcgcgc tcaaggtgcg
     8761 caagcccggg ctggtgatag gcgtcggggg gtgcgttgcc agccaggaag gcgcggccat
     8821 ccagagccgg gcgccccacg tggacgtggt attcgggccg cagaccctgc accggctgcc
     8881 tgaaatgatc gatgccgccg cgggtcccgc acgggttccg ctcgtggatg tgagctttcc
     8941 ggagatcgag aagttcgacc gtctgcccca gccgcgggct gaagggccct cggccttcgt
     9001 ctccgtgatg gagggctgca gcaagtactg cagcttctgc gtggtgccgt acacccgcgg
     9061 agaggaggtc agccgaccgg ttgccgacgt gatgcgcgaa gtgatccagc tcgccggtca
     9121 aggggtccgg gaggtgaacc tgctcgggca gaacgtgaat tcctacctgg gccccatcga
     9181 cgacgaccat gcagacctgg cggagttgat ctactacgtg gccgaagtcg acggcatcga
     9241 ccgcatccgc ttcaccacct ctcatccgct ggacttcaac gattcgctcg ttcgcgcgtt
     9301 cgccgacgtt ccggaactgg tggatcatct gcatctgccc gttcagtccg ggtccgaccg
     9361 ggtgcttgct ctgatgaagc ggggccacac cgtgagcgag tatcgtgaaa agatcgcggc
     9421 cctcaaagag gtgcgagggg gcatcagcct ttcgtcggac ttcatcgtcg gcttccctgg
     9481 cgagaccgag gcggacttcc aggccaccat ggacctcgtc gccgagatcg ggttcgatac
     9541 ctcgttcagc ttcgtctaca gcgcacgccc cgggactccg gcggccgccc tgccggacga
     9601 cacgccggaa gccgtcaagc gcgagcgcct ggcgctcctg cagggcctgc tgcgctctca
     9661 ggcggatgcc atcgcctgcg acatggtcgg aacgcggcag acggtgctgg tcacgggcga
     9721 gtccaggaag aatcgggaag agctgcaggc gcgcaccgag aacaatcggg tcgtcaactt
     9781 cccctgtccg gaccgtcgcc tcgttggcgg cttcgtggac gtggacatta ccgaagccct
     9841 gccaaactcc ctgcggggcg tgatggcgat cacatagggc gcagacgggc gtttggggcg
     9901 gcgaaacccg atttgacagg taactgcgcg ccgatatcct agacaaccct cttctgcaca
     9961 gggcgcgcat tgagcgaaga cgattcacgg gtggtggcgc tcaccacgaa tgtcagcctg
    10021 gaacccaacg aaccccgccg attggcagcc ttgtgcgggc cgttcgacca gaacctcaaa
    10081 cacctcgaga ggcggctggg cgtacatatt cgcagccgcg gcaacctatt ccagatcagc
    10141 gggccggagc agcgtgttca ggcggccggc gcgctgctgt cccagctata ccgggagacg
    10201 ctcgagcacg agaccatcga cccggacatg gttcacctgt tccttcagga atcgggcgtc
    10261 gacgagctgc tcgcgcgtca gcgcgggcca gacgagatgc ccgcggacgg ggagggtgcg
    10321 gtcatctcga ccgggcgaaa atcagtcaag ccccggggcg gcaaccagac ccgttacgtc
    10381 gagcggattc gcggccacga tgtgaatttc ggcatcgggc cggccggaac cggcaagact
    10441 tacctggcgg tggcctgtgc tgtgcaggcg ttggaaagcc agcaggtgag ccggattctg
    10501 ctggtgcgcc cggccgtgga ggcgggcgag aagctgggct ttctgcccgg ggatctggcc
    10561 cagaaaatcg acccctacct gcgcccgctc tacgacgccc tgtacgagat gatgggcgtg
    10621 gatcgcgtca acaaggccat cgagcgcaac atcatcgagg tggcccccct tgcctacatg
    10681 cggggccgga cgttgaacaa tgccttcatc atcctcgacg agagccagaa caccaccatc
    10741 gcccagatga agatgtttct cacgcgaatc gggttcggct ccactgcggt gatcaccggc
    10801 gacatcaccc agatcgacct gcccaaaggg cagaagtcgg ggctggtcaa cgtgcgcaac
    10861 gtgctcagcg gcatcgacgg catcagcttc acccacttcg gctccaagga cgtggtgcgc
    10921 caccccctgg ttcagaagat cgtcgacgct tacgaggcat atcaggagcc tcatgcaagc
    10981 ccggacagcg accgtgatgc cggttgaagt gcagatcgca tccgtccagg cggggcttcc
    11041 gtccgtcgcg cgcatcgagg gctgggcgtc cgccgcgctg gacgagctcg gggacgaagg
    11101 cgagccgccg gagctgtgta tccgggtcgt cgacggagag gagtcgcggg agctcaatgc
    11161 ccgtttccgc ggtcatgaca aaccaaccaa tgtgctgagc tttccggccg acgtcgatct
    11221 gccggaagcc aagctgctcg gagacgtcgt catctgcgcg cccgtggtag gcgtcgaggc
    11281 ggccgaacag ggcaagtcgc cggaggatca cttcgcgcac atggtcgtgc acggggtgct
    11341 tcatctgagc ggctacgatc atgagcaggc gggggaagcg gagctcatgg aagcgatcga
    11401 aagacgaata ctggagcggg tcggcatagc cgacccctac gccgagcgat gagcgaggtc
    11461 ggaggatcgg agcagcagcg gcgttccttg ctggactggc tcgcagacct tttcaacgac
    11521 gagcccacgg accgttccga gctgatggag acgctgcgcg atgctgccga ccggcagatc
    11581 atggatagcg aggcgctcaa catcattttc ggggccctgc aggtgtcgga catgcaggcg
    11641 cgggacatca tgattccgcg gacacagctc gtgtatctgg aggactccaa ggcccccgag
    11701 gagctgctcg ccaccatcat cgagtctcag cattcgcgct ttccggttgt cggcgatgat
    11761 ctggacgacg tcaaaggcat tctgcacgcc aaggatctgc tgccgctggc gctctccagc
    11821 ggctcgggca gcaatggctt cgacatcaag gactgcatcc ggccggcaac gatcgttccg
    11881 gaaagcaagc ggttgaacgt cctgctgcag gagttcagaa gcacccgcaa ccatatggcg
    11941 gtcgtggtgg acgagtacgg tcacgtttcc ggcgtggtga ccatcgagga cgtgctggag
    12001 cagatcgtgg gcgagatcga ggacgagcac gacgtcatcg acgacagctt cgtcaagcag
    12061 ctcgattccc gaacgttcaa cgtgaaggca acgactacca tcgaggattt caacgagcac
    12121 ttcgacctcg agctcgacga cgacgagttc gacaccattg gcggcctcgt gctcaaggcg
    12181 ttcggccacc tgcccgatcg cggagagtcg gtgcggatcg gagatctcga cttcaaagtg
    12241 ctcaatgccg attcgcgaag agtcaggctg ctgcaggtca agctgcgcaa atgaagcccc
    12301 tcctcgcgct cgcctgcggc gcgctggtcc ctctctctct tgcacccttc ggctggtggc
    12361 cgcttgggct cgtcgccgta ggtggctggt ttgctttgct gcagtcgagt gatcggggcg
    12421 ggctgcttct gggctggctg tttggtttag gcaagtacgc ggccggcgta tcctggatct
    12481 acgtcagcat caacgtttac ggcaacgcgc cgccgccgct ggccgccttc ctcgtcgctc
    12541 tgtttgccgg tgggctcgcg ctgttcccca tggtaaatgc ctgggtgttc cagcgcctgc
    12601 ggaccaaggg cgcgccgctc gtcaacgcct ggctgttcgc ctgcctgttc gtggcgttcg
    12661 agtggctgct cacctggttt ctgaccggct tcccctggct ctatcccgcc tatggtcacg
    12721 tcgagacggt cctcggcggg ttggcccccg cgggcggggt gatgctggtg agcctcgggc
    12781 ttgccgggtc ggcctgctcc gtcgccgcgg cccatcagtg cgcgcgacgg ctgcccgtgc
    12841 tcacggcggc tctggcgccc tgggttgtcg gtgccgttct gggcgcggtt tcctgggtcg
    12901 agcccggggc cgttcgcacc gtagcgctcg tgcagggcaa cgtggatcag gccgtgaagt
    12961 ggctgcccga gaaccggcgg cccatcatcg atcgctaccg ggggctgtcg gaggaccatt
    13021 ggggcgtgga cgtcatggtc tggccggaag ccgccatcac ggtgatggcc catgacgccg
    13081 gggaggttct gggtgatctg gagcgcgagg gcgagcggtc gggcaccgct gtcgtgctgg
    13141 ggctgccggc ggtggagcgg cttgccgacg atcggatcgt cctgcgcaac acggccattg
    13201 ccttagggga tggatcgggg cgttacgtca agcggcggct cgttcccttc ggcgagtacg
    13261 tcccgttgga ggggtggctg cgggggctca tcgagttctt cgatctgccc atgtcgcgcg
    13321 ctgcgcccgg cgattggcga cagcctctgc tcagggtcgg tcaggagcag gcggcgctgg
    13381 ccatctgcta cgagatcgtc tatccggatc tggtcagggc ccaggccgtc ggggcagacg
    13441 tgctggtcac catttccaac gatacctggt tcggtgccag catcggcccg ctgcagcatc
    13501 tgcagatggc gaggatgcgc gctctggaaa acggccgctg gctgcttcgg ggaaccaaca
    13561 acggggtgac cgccatcgtc gaccaccggg gcagattggt tgcgcggctg cctcagttcg
    13621 aggccggcgt gctgcggggc gagtaccgac tcgtggcagg ccgaacgccc tacaaccgtc
    13681 atggtgatgg gccggttctg gtgctggttg ccttcggtct cggcgtcgcc ttctggcgca
    13741 ggctcggcgc ggcgcgtggc gccgacgggt agtgtagtat gcggcgcgtg cgcacccgtc
    13801 tacgctacac ctggctgatc ccgcctctgt tcctcctcat cgggggctgc ggctttcagc
    13861 tgcgcacatg ggatctcggc accagcgtgg agagcttcta cgtcagggac gccagcaaca
    13921 atccgctggc atcgccgctt cggcgggccc ttcgtcaggc cggcgtcgac gaggcgggca
    13981 acgcttcgga tgcccaggtg gtggtgctgc tgctgaacgc ccagcgggga cggcgcagcg
    14041 tgtcggtcac cggtcaggct cgggcggccg agtacgaaat ttcgctcggc gttcagtacg
    14101 gggtgctcga cgggcaggga caggagctca tcgcgccgcg ctgggtccgc gggacccgcg
    14161 tgttccgggt agaccgggac aacatcgtcg gcaccagcga ggagcaggcg ctgctcgagc
    14221 gggaaatcca gaacgatctc atccagcaga tcgtcagggc gctcaacacg gtttccggcc
    14281 cggccggccc ggcgggtcag acggccaatg ccgcttaggc tgtcggacct gcaaagccgt
    14341 ttggccgccg gccttgcgcc cgtgtacttc atctccggtg acgagaccct gctggttcag
    14401 gaagcctgtg acgcgatcct gggcaaggcg cactcggagg ggttcacgga acggtccgtg
    14461 ttccatgtcg agaagggatt ccgctggaac gacattcttc aggacgctgc gagcctgtcg
    14521 ctgtttgccg agcggcggat catcgacgtc cgggtgcccg gcaacacgtt cgaccgggaa
    14581 gcctcggagg ttctgcgtac ctactcggca gagccctcgc cggacaccct tctgctcatc
    14641 cgctcggcgc ggctcgagcc gcgacaacga tcgtcggcct ggttcaaagc cctggatgcg
    14701 gccggcgcgg tggtcctcgt gtggccggtg ggcattgccg agctgccgcg ctggctggcc
    14761 gggcggctgc gcgcagccgg gctgtcgctg acgccggacg cccagagcta cctggcgcag
    14821 caggtggagg gcaatctgct cgctgcggtt caggaggtgg agaagctcag gcttgccgat
    14881 ctgccccagc ccatcggcag cgaggaactg atgaggcaga tggaagactc tgcccactac
    14941 gactccttcg agctcatcga tgcggttttc gcgggcgatg gggcaagggt gagccgcata
    15001 ctggccaatc tccggcagga gggcgtttcc ctctacgccg ttctggctgc actgactggt
    15061 cagctccggc gtctgcccgg cgggcggatg gcgccccagc gccagcgcct ggtcgacggc
    15121 ttcatgcgtc gcatgaggtc cccgcgcaac ctcgacaggg ccctggccca gtgcgcgctc
    15181 gtggacgctc agggtaaggg ccagctgctc ggggatgcct ggctttctct ggagaacgtc
    15241 tgtctgcggc tggcgggaca gcgcggcctg ccgtctctcg aggaggagct gccttcgctg
    15301 cgcagagcct gatcgtgccc ggcgccaagc gggcgatcag caggcccggc tcacaccccg
    15361 ccccagtccc gcgcctcggc ggtctgggtc atgaagttgg ccacccagcg gtggtagttc
    15421 tgcaccgcgg gctcgttgcg gccgaagatg aaggcggcgc gcgcaggggc acgaagcccg
    15481 gcctgaatgg tctggccgat ggcgtagtcc tcgtcgcgga ccgcctggag caccatgtcg
    15541 gagaaattgc gggcagcggc ctcggcggcg tccgttgccg gtttctccgc caccaggatg
    15601 ctctgccggg tgacggtgcc gaacgggtcc tctcccggga agatctggct gaccaggcag
    15661 tggtcgccga ggattcctga gacagacagg ttgggaaacc cggagtggat caggcgaatg
    15721 tgctcgagcg gagcccactc ggactccggc gtttccgcca gcgtccccag cgtgcggcgc
    15781 cccagggtga gccgctggtg ggggccgtag gtatccaaca ccagcaggtt tcccacggtg
    15841 tgttgcccca cggtggcacc gtgcagcggg ttgtgatgat agatctccag gtagccgtcc
    15901 atggcgactt tccagcccgg gccttcgaga tcccgctgct cgtacagata ccaatccgtg
    15961 agatgcagcg aacccagctg ctcggcaaac ccgccgagcc aggcgtccgc gtcgaactgc
    16021 tcgcccgggg tgagtatcac ccagatgacg ccggcccgct cggcgcaggc aagctccgtg
    16081 agcgcgtaag cctcggggtc caggtcgccg aaggtctccg ccgcgtaccg gcccgacagt
    16141 cggccctcga agtcgaaagt ccaggcgtgg tagggacagc tgatgaccct gcgctctccg
    16201 gcaccttccg ggcacagccg tgccccccgg tgagtgcaga cgttgacaaa agcacgaacg
    16261 gtgtcgtctt ccccgcggat gagcaggacg ggtacgttca tgaccgtcat ggcccggtag
    16321 cagccgggct cgggcagctc gaggctcaat gccagcgcca gcgggagatg catgaagatg
    16381 cggtcccgct ctgcctggta gcgcgactgc cccacatagg cctccaccgg catctcgagg
    16441 gtggatgccg cctggtcggt cgtcttgttc acgaagtgct cgagcgcgcg gcgcgccagc
    16501 gacatcatct cttcatcgtg ggtcaagact tcaagctccg ccatgactcg cctcgaacgc
    16561 ctgccatgcc tccagcgcct cggggtagtc ggcggcgtgt cgctcggtgc ccatgaccac
    16621 gcggtcgggg cgcgtcaggc tggccccgta ctcggaaacg ggcacgtagt acagaaagct
    16681 gatctcgcgg ctggcgaaac gccagatgcc ggcttctctg cgatagctgt cgtgatagcg
    16741 catggccgca atgctcatca cgccgctggg cgtggtctcc gcgtggctgt gcaccacgcc
    16801 ggtggcgcgg tcggggtcgc tcggatcgat gttgacggtc acgtcgtggg tccagtggta
    16861 acccgggccc cggataccga gaatccgaac gaacatgtcg tggatggcgt tccggcccgt
    16921 ggcgatcatg gcgccgttcg gtgaggcgaa cacggcatcc tcggtgaaca gatcgtccag
    16981 cgccggcatg tcgtggtggt cgcaggcaac ggcgtaggcc gacaccagct cgcggatggc
    17041 ttctctggac tccagacggt ccactcgtgt gcgcaattcg tccagctcgc tcaagatcgc
    17101 ctccccagct cttcatcgct tgattcaact gaatatagag ggcgctcacc acgcgatcaa
    17161 caaggcccgc agccggcccg cgcccaatcg gctggagagg gcctgcagac ggcggaaatc
    17221 gggcgttcga gggggcttga gccggtctgc cgcccgggcg tcctcgcgga tttgaccaac
    17281 tctttgccca ggtggtgcga aaacgccggc ctggcgggca gcgagattaa tcgcccgcat
    17341 ctgctgctct tttaccggtg gttccttaca ataaacggct cgcccgcagt tgaggaattg
    17401 cagtgacggt aaaccaatcc ggcctgtccg cggacatggc tccggacacg acggttagtg
    17461 cccgggaaca gttcggcgtc gagcacgact tccaggtgcc ggcgttcagc aagcgatcgg
    17521 actacgtgcc ggaggtggac gtggattacc gtttcgatcc ggacaccacg cttgcgatcc
    17581 tcgctggttt cgcccacaac cgccgggtga tgattgccgg ctaccacggc acgggtaagt
    17641 ccacccacat cgagcaggtg gccgcgcggc tgaactggcc gtgcatccgc gtgaacctcg
    17701 acagccacat cagccggatc gacctgatcg gcaaggacgc catcgtgctg gaggagggca
    17761 agcaggtcac cgagttccgc gaaggcatcc tgccctgggc gatccagcat ccggtggcgc
    17821 tggtattcga cgagtacgac gcgggccgcc ccgacgtgat gttcgtcatt cagcgggtgc
    17881 tggaggtcga aggcaagctc acccttctcg accagaacaa ggtcatcacg ccccatccgt
    17941 tcttccgtct cttctccacc accaacacca tcggtcttgg cgacaccacc gggctgtacc
    18001 acggcaccca gcagatcaac caggcccaga tggaccgctg gagcatcgtg accaacctca
    18061 actacctcga gcacgatgcg gaggtggata tcgtgctggg caaggccaag cactacgcgg
    18121 agaccgagga cggcgcgcgc acgatctcaa acatggtggc ggtggccgat ctcacccgca
    18181 aaggcttcgt caacggcgac gtctccatcg tgatgtcccc gcggacggtg atcacctggg
    18241 cggagaatgc ggagatcttc ggagacatcg gttacgcctt ccgcgtgacg ttcctgaaca
    18301 agtgcgacga gctcgagcag cccatcgtgg ccgagtacta ccagcgctgc atgggcgagg
    18361 atctgggtta cgccacggcg ggaattgcgc tcaagtcggc ttgagtcctg acaagcgccg
    18421 atgtctgagg aagaacaccc gctagagccg ttcaagcggg ccaccacagc caccatccgg
    18481 gccatcgcgg agaacgacga gctggaggtc accttcggcc aggggccgcc caccgtgcgc
    18541 ggcaaccgga tccgggtgcc ccttccggcg gtggggtccg caccgcagga ggtggacgcc
    18601 atccgcggca tgggtgacga gttcgccctg cgcatccgct atcacgacga tgacgtgcat
    18661 cgcagccgcg cgccgcgatc gggcccggcc caggaaatgt tccagtggat cgaggatgcc
    18721 cgcatcgcct ccatcggctc cctgcgtatg gagggtgtcg cccagaacct ggatgcaagc
    18781 ctggaagtgc agtgccagca ggcggccttc gacaccatca ccgctgaggc tgacgccccc
    18841 ttgagcgtcg ccgtcgggct catcgtccgc cagcacctca cgggccgcga cctgccgccg
    18901 tcggccgaga acgtggtccg tttctggcgc gcctacgtcg aggagaacgc gggcgacgac
    18961 atcgacgccc tgcgcgattg cctgctcgac caggcggagt ttgccgagcg ctgtcgggcc
    19021 atcatcgccg acctcggcct gggtgccgag ctcgacgagc cgccggaact cgacgagagc
    19081 caggacgata ccgacgccat ggacgaggac caggacgcgg attcggacat cagtccggac
    19141 gacgtggttc tcgacgatga ctccatgatg gacgagtccg ccgagggcga cgccaccatg
    19201 atggagatgg acgccgacat ggacatggac gagctcggcg cggagatgga gccggacgaa
    19261 gcgcccacgc agatgcccga cgagagcggt cggatccgca tggacttcaa ctacgaggcc
    19321 tacacccagg agttcgacga gaccgtccgc gccgaggagc tgtgcgacgc cgacgagatg
    19381 atccggctcc gcgcgctgct cgaccagcag ctcgtccccc tgcagcatgc cacctccaag
    19441 ctcgccaacc ggctgcagcg caagctcatg gccaagcaga gccggacctg ggagttcgat
    19501 ctggacgagg ggatgctgga cgcctcgcgg ctatcccagg tgatcatcga tcccatgaac
    19561 ccgctggcct acaagcagga aaaggagatg cagtttcgcg acacggttgt cacgatgctc
    19621 atcgacagct ccggatccat gcgcggacgg tccatcacca tcgccgccat gtgcgcggac
    19681 attctgggcc gcaccctcga gcgctgctcc gtgcgggtgg aggtgctcgg tttcaccacg
    19741 cgagcctggc ggggcggcca gtcccgggag aagtggatcg cctcgggcaa gccgtcgaac
    19801 ccggggcgcc tgaacgatct ccgccacatc atctacaagg cggccgacga cccctggcag
    19861 cgcacgcgcc gcaatctcgg cctcatgatg cgcgaggagc tgttgaagga gaacatcgac
    19921 ggcgaggcgc tcatctgggc ccacaatcgc ctggtggtgc gtcacgagga gcgcaaggtg
    19981 ctcatggtca tctccgacgg cctgccggtg gacaactcca cgctgctggt gaacccgagc
    20041 aactacctag agcagcacct gaagtacgcc atcgaccaga tcgagaacta ctcgtcggtg
    20101 gagctcatcg ccatcggcat cgggcatgac gtgactcacc attaccagcg cgcggtcacc
    20161 atcacggatg cggagcagct cggcggtgcc atgaccgagc agctggcgga gctcttcgag
    20221 gccaagcgct gacgggccgg gccctgggaa gccgacttca aacgggagcc tgcggtcccc
    20281 gacacgacga ctaaccgacc acgaagttca gcaggcggtt gggcacgtag atgacgcgac
    20341 gcacttcggc gttgtccaga tggcgggcca cgttctcctc ggcccgggcc agcgcctcgg
    20401 cttcagcctg gtcggtgccg ggtgcaaggc tgatggtgcc gcgcagcttg ccgttgacct
    20461 gcacggccac cgagaccacc tcgtcgcgga ccttggcctc gtcgtaggcc ggccagtttg
    20521 cgcccgcgaa gatgctcttg ccgtgtccga ggcgctccca cagctcctcg gccaggtggg
    20581 gggcaaaggg cgcgatcata accaccagcg gctccacctc cgccactgcc ggttgcctgc
    20641 cgccggcgcg cacggcgttc aggcactcca tcatggcggc gatggccgtg ttgtaaccga
    20701 gctcgggaat ctgctcggtg gcctgcttga tggtcttgtg cagcttgcgc tccacgtcct
    20761 cgtccgcagc ggtgtcgggc ttggcggtca caaccgcatc ccacagccgg tgcaggaacc
    20821 cgaaggggcc ctggatgccc tcgtcccggt agtcgccgcc ttcctccagg gggcccatga
    20881 acatgaggta ggtgcgaaag gtgtccgcgc cgtactggtc gatgatggcg tccggggtga
    20941 tgacgttgcc ccgggatttg gacatcttcg cgccgttgcg ggtgatgagc ccgtgggcgc
    21001 ggaacttcac gaagggttcc tcgaaatcga tgtggccgag gtccttgagc accatgttga
    21061 tgaaacgcga gtacatcagg tgcagcacgg cgtgctcgtt gccgccgatg tagctgtcca
    21121 ccggcagcca gcgggcggtg atcccgtcgt ccatgggcgc gtcgttccgg tccgtggacg
    21181 ggtagcgcag gaagtaccag gaggagtcca ggaaggtgtc ggacacgtcg gtttcgcggc
    21241 gcgccgggcc atcgcagccg gggcaggtgg tttcgtacca gctggccacc cgggcgagcg
    21301 ggctcacgcc ggtgtcgtcg ggcttgaagt cgtcgactct gggcagctcc accggcagct
    21361 gatcctcagg caccgccacc gcgccgcagg catcgcagtg cacgatgggg atgggcggtc
    21421 cccagtagcg ctgtcgggag atgcaccagt cccggagccg atagttgacc ttgagctcgc
    21481 ctaggccccg ctcgcccagc cacgcggtga ccgcgcgctt gccgtcagcc acgccgagcc
    21541 cgtcgaaggg gccggaattc accagcacgc cgtcgccggt gtatgcctcg gccagcggcg
    21601 tgtcggcccc gtcgccttcc ccggccacca cgcggactat ctcgatgccc atggcggttg
    21661 caaactcgaa atcccgctcg tccccgcccg gcaccgccat tatggcgccg gtgccgtact
    21721 ccatgagcac gtagtcggcg atccacacgg gaatgggcgc cccgttggcc gggttgcggc
    21781 agtagccgcc ggtgaaaacg ccggtctttt ccttgtccgc tttctgccgg ctcaccagat
    21841 ccgtcttcgc cgcctgggcc tggtaggcgg ccacggccgc ctgctgcgcg tccgtggtga
    21901 ttgaggcgac cagcgggtgc tccggggaga gcaccatgaa ggtggcgccg aacagggtgt
    21961 ccgggcgtgt ggtgaagacc tcgatcacgt cgccgtcagc tgttacggtc agggggtcga
    22021 cggtgcccgg gtccccgttg cccccctcga tcaccggaaa acgcagggtt gccccctcgc
    22081 tgcggccgat ccagttctgc tgagccttac gggtgctctc cgaccagtcg atgtgctcca
    22141 ggttgtccag caggcgctgg gcgtactcgg tgatgcggaa gaaccactgg gcgatgtggc
    22201 gctgctccac ggcgctgtcg cagcgctcgc acagcccctg gatcacctgc tcgttggcga
    22261 gcaccgtcat gcacgagggg caccagttca ccggcgcctc tttccgctcg gcaaggcccg
    22321 ccttgaaaag ctggaggaac agccactggg tccacttgta gtaggcgggg tccgtggtgt
    22381 ccacggtgtg gtcccagtcg tacatgccgc cgatagcctt cagctgccgg gtgaagttcg
    22441 cgatgttgct ggggataagg tccatggggt ggacgccctg cttgagcgcg aagttctccg
    22501 agtggatccc gaaagcgtcg aagccgatgg gctcgaagac gtcgcgtccc gtcaggcggt
    22561 ggaagcgccc gtgcacgtcg gcgcccgtga aggcgtacat gttgcccacg tgcagcccct
    22621 cggccgaggg gtaggggaac atcatcaggt tgtagtaggg cgactgggcg ctacgaagct
    22681 catcggcagt gaacgagttg gtaccgcgct cttcccagag ccgctgccac ttggcctcca
    22741 cctcgccggg cgcgtactga ttttccgctt cgctgctcat cccgctgcgc tcgcttaact
    22801 tcctagccga tgacctggag tcgctggatc tctggccggc ccgacatcag cccggcgaag
    22861 ctcgcaccgg aggccttgat gtgctcggtc tggccgtggg cggccagcgc ttcctcggag
    22921 tcgtacagct ccatcatcag gtagttgccg tcggcgtccc tgcacagcgt gtagagctgg
    22981 ttgccgggtt cgttctcccg cacctgggcg gcgagtgcga gcatggcctt ctcgaaatcc
    23041 gcctctctgc ccggggttac gttaagcttg gcgatcacgg cgatcatggt gctgccctcc
    23101 tcaaaaaagg aggcgagcat agcaagggat ttccgctaat gcacaggcgc gggctcattg
    23161 ggctgctgga ggattacggg cgcaggcacc cggcggaatc tgcagtggtg gagcggtttc
    23221 tggcgttcgt tcgcgagcac gagcgctgct tcgagcggga ctgctttccc ggccacgtca
    23281 ccggctccgc ctggctggtg aacggggccg gtacccacgt gctgctcacc catcacaaga
    23341 agctgggccg ctggctgcag ctcggcggtc acgccgacgg cgagacagac cccctattgg
    23401 ccgcgacgcg ggaggcggag gaggagtccg gccttgccgt cgagccggtg ggagaggccg
    23461 ttttcgacgt cgacatccac gagattccgg cccgtggccc ggatcccctg cacctg
//
LOCUS       DBNHLM010000328        15907 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_947406, whole genome shotgun sequence.
ACCESSION   DBNHLM010000328 DBNHLM010000000
VERSION     DBNHLM010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 15907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15907
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_947406"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="AC16QF_15155"
     CDS             <1..189
                     /locus_tag="AC16QF_15155"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6449947.1"
                     /translation="YADPKVRETRARAVPLGRLGTAEDVANAVAFLDSEAGAYVNGHE
                     LVVDGGVTHTLLKHLPRD"
     gene            189..866
                     /locus_tag="AC16QF_15160"
     CDS             189..866
                     /locus_tag="AC16QF_15160"
                     /inference="COORDINATES: protein motif:HMM:NF018038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR6449948.1"
                     /translation="MRNVMLIGGIYHPFEEAAPVLARLLESLGIESVITSDVSEAVAE
                     LESADLFTIYALRWRMLNDEKYVPFRKQWAFELPEEDAQAIENFVEAGGGMLGLHTAS
                     ICFDTWYEFPRLLGGSWRWGETFHPPEGNVEVRAVADHPATRGVTDFGLHDEIYHHLA
                     IGNESKVLLEGRVPDGDWHPISWAHEAAAGRVVYSALGHDGRSLGDPHHARFIRQAVA
                     WLLGEDE"
     gene            863..1624
                     /locus_tag="AC16QF_15165"
     CDS             863..1624
                     /locus_tag="AC16QF_15165"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF048554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6449949.1"
                     /translation="MRPIAGMSILVTGASSGLGAGAAAYFAERGARVTISARREDRLA
                     AVKEAIGDAAAMVVGDVTSAADRQRMIDEAVRHGGGLDALISNAGNMYRGPITDLDEQ
                     ALLDIFHSNVVAGMLLTGLAVPELEKRQGAVIFVGSIHTMRAFPGASPYAATKGALQT
                     LTRVLAAELGERGIRVNCVIPGAVPTEIYQRAGLLSDEDARARIAGMASMHPLGRVGT
                     EEELAEAMAYLIQAEWTTGAELVVDGGLGLGATNA"
     gene            1698..3083
                     /locus_tag="AC16QF_15170"
     CDS             1698..3083
                     /locus_tag="AC16QF_15170"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6449950.1"
                     /translation="MAESQPKPGEARCPGISVQDLMDRDSRTVPAYLREHSYAFLGDE
                     DIPFARYTSRDWFDKEIEHMWPRTWQWACREEHLPAPGDYYVYDVGPYSVIVLRDSSG
                     AIRAYRNACMHRGTKLKPSFSTGSADSIRCPYHGWQWNLEGELQSFPCEWDFPHVDPD
                     AVGLSSVRAELWGGFVFINLDPEAVPLREYLHPLPEHVDSSVFEQRYVAIHIQKELNC
                     NWKIASEAFLEAYHVMETHPQLMRTNGDANTQYDFYGDHVNRLINVAGSPSAYLEEPI
                     TEQEVLDLFLVGDREDIGEALVVPEGGTARQVMAEHFRESVNASGGADLSEVCDSEIV
                     DTIAYFVFPNQQFFSGVSFPIVYRFRPLGMSHDRSLFDFVILKTRPNDGSPAPTAEPV
                     RLTADESYTLVPGMDPYVGHVFDQDTGNVQAAQEGAMATDKAGATLGNYQEIRIRHFH
                     QTLGKYVAGEL"
     gene            3093..3788
                     /locus_tag="AC16QF_15175"
     CDS             3093..3788
                     /locus_tag="AC16QF_15175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6449951.1"
                     /translation="MTDVLITGAARGIGFELARQYAEAGDRVYATSRNPDGAAQLSAL
                     AADFPDNVSVHRMDVGDMGSVQGCAAELNAAPIDVLINNAGVWGGLETQTFQNMDYEN
                     WAFEFNVMAMGPFRVIQTFLPNVLAGRDKKIVTLSSQVAAHVYDHVIGYSYASAKAAV
                     NRLMTALATELQDQGIIVVPMHPGWVKTDMAGDVADIYPSESAEGVRRVIAGMTMKDT
                     GKFLKWTGDIHPW"
     gene            3858..4946
                     /locus_tag="AC16QF_15180"
     CDS             3858..4946
                     /locus_tag="AC16QF_15180"
                     /inference="COORDINATES: protein motif:HMM:NF024375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6449952.1"
                     /translation="MNTGYNPEFNPTGIEGGIDTNALPWIDLPQAPGVSIKPLRASSE
                     SGMFSTVVRVADGSELSSIVYLGAMDMIVLSGRLTYPSGPMSGTLEPGTWGYVPANAR
                     VERFIAEQDTEFLANFYGPVAFLADDGRTVSSLLTSLDIQAAARDRGINLVPNTLAEC
                     MQERPPAYQGPVEPLAIAGSDSKALVVAAEGVAADAGRAVHPHFIDTRAVPWIVNPDT
                     PDLGLKILRVSEETGITSLIVRHNGVAGPHYHLGAADFMVLSGHIGYRAGPPEGYGPG
                     VWFYEPAGARHEATQRIGEEDLIYTANVYGPLQFDEGPDTPIAAVLSWMQYKAMADAG
                     GVDLVHSTFDNDASLLAWAPIGSEGARL"
     gene            4987..5538
                     /locus_tag="AC16QF_15185"
     CDS             4987..5538
                     /locus_tag="AC16QF_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449953.1"
                     /translation="MDVNKGIRVCEEIAASETDPRKRHNLEMVIAHMRAEARCDVQGV
                     LDTLVDEPCYVWHSAPDDPLLNPRGSREAVADFYDRMIVRTGAHRLEWDITRVMVDEH
                     AVLTEGVMRVAFPGWALAEMDIAVDDPDAYYVAEGITTVIWPVDPETGLLVGEEVYEY
                     DKMAGIADRKIAPSDILPLSSAA"
     gene            5566..6018
                     /locus_tag="AC16QF_15190"
     CDS             5566..6018
                     /locus_tag="AC16QF_15190"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6449954.1"
                     /translation="MTDPVTELRDAIDRQTIADLQNRYAYAIDAGRYDDLDGVFTPDA
                     VADYGPAGRVEGVEAIKATCRHALEPLTAAQHINTNHWAVIEGDHARAGCYLHVHQHR
                     ESTPGGDHLEMGGRYEDALIRTPDGWRITSRKLTILWSQGNPEVRWER"
     gene            complement(6015..6938)
                     /locus_tag="AC16QF_15195"
     CDS             complement(6015..6938)
                     /locus_tag="AC16QF_15195"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR6449955.1"
                     /translation="MRQALLETNLGLPNRRSGKVRDLYDVTLDGGEEALLIIATDRIS
                     AFDVVMANGLPGKGIVLTQISRFWFDHFAGEVDHHLISTDVADVPGLSDAQREALAGR
                     IMLCRKTRVVPIECIARGYITGSGWKDYQRTGAVCGIELPEGLRNSDRLEEPLFTPST
                     KADTGHDENISFEEGVGIVGRELMDWLGDTTLRLYAKARDYALERGIILADTKFEFGQ
                     LPGREEPILIDEIFTPDSSRFWPADEWEPGREQPSFDKQIVRNYLETVVAAGEWDKTP
                     PGPALPDEVVEKSIGRYLEAYQRLTGSTLDL"
     gene            complement(6975..7742)
                     /locus_tag="AC16QF_15200"
     CDS             complement(6975..7742)
                     /locus_tag="AC16QF_15200"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6449956.1"
                     /translation="MGRLKGKVAVITGGAGGIGAAAGRLFAEEGADVLLVDVDEKALE
                     ETVRGIGSNRVSYFVGDVTASADNAAMVACAEERYGGVDVLIANAGIEGDVAVPIVDY
                     DEARFDQVLGVNVKGVFLGLKSAIPAMARRGGGSIVITSSVAGVGGSPGMSAYVTSKH
                     AVIGLMRSAARECAGQNIRVNTVNPSPVETRMMRSIEEGIAPGGGEQVKSLMQSRIAL
                     QRYAEPEEIAKVMLFLASDDSAWVTGSVYMADGGMTA"
     gene            complement(7769..8992)
                     /locus_tag="AC16QF_15205"
     CDS             complement(7769..8992)
                     /locus_tag="AC16QF_15205"
                     /inference="COORDINATES: protein motif:HMM:NF004609.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HIR6449957.1"
                     /translation="MKGFTTKIVHSDRLEVIEHGSLHKPVHSTVAYGYDDARDLAAVF
                     QGTKAGYTYGRQVNPTVEALQTKISTMEQGLQTVCFSTGMAAIGTMLFALLRNGDHFV
                     SSSFLFGNTNSLFNSFSTHGVDVTFVDATDVDAVAAGINERTRLVFVETIANPRTQVS
                     ALEEIGKLCAERGLIYVVDNTMTSPYLFLPASVGASLVVNSLTKYIGGHGNALGGAVT
                     ELGNFDWADFSNIYDTYKSGAAEKWAITQIKKKGLRDFGAALAPEAAHHIAVGSETLG
                     LRLERQCANAAAMTAMLDAHPKVKAVYYPGLKQHPQHERASRLFRHPGALFSFELVPE
                     VDIWNFMNALDVIIKSSNLGDNRTLAIPVAHTIYYEMGPERRASMGIDDSLIRISVGI
                     EESEDLLGDLERALG"
     gene            complement(9011..11263)
                     /locus_tag="AC16QF_15210"
     CDS             complement(9011..11263)
                     /locus_tag="AC16QF_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HIR6449958.1"
                     /translation="MSESSTADASSPRESFSRLLNRKSAGSGLVGASILLVDDKPELL
                     NSLYQLMVLHGYEPDKALGGQQALNLLAEKSYDVVLLDLIMPGVSGHDVLDYAARNEL
                     TCKIIVVSGDSSFSGVKHALHCGAFDFVKKPYEAGELIATMETALRQCQLEAQNELME
                     EKVKESEELHRYIVNSSPDLVYMLDRNGCFTFLNDRVESLLGFSRDELIGKHYTELVD
                     DDHLELARNVFNERRTGDRAVSNAEMRLKSRHARRGARLFHAHAIWTELTAMGVYSDP
                     KERTRENFVGTYGIARDISERKEAQEVISFQAYHDLLTHLPNRALLKDRLSLAITQAR
                     RSKRRLAVMFLDLDRFKIVNDTLGHSMGDRLLKAVANRLQSCLRRGDTLSRFGGDEFT
                     LLLPEVRTRDDVVVIATKILDRLGGPFVIDGLELFVGASIGVAMYPEAGETEEALIQN
                     ADIAMYHVKGRGKNGYQFFSEEMNHKYSTRLSMERELRNGLTQEELVVYYQPQVALKT
                     GEIVGVEALVRWRHPERGLVEPGDFLPMAEETGLITQIDTFVQRRACTDVAGWERAGL
                     GSIRLSVNLSAPQIEQEDFVDNFVADMAAAGLDPSRVKLEITENILMQDMEVVIPKLK
                     QLRQLGVRIAIDDFGTGYSSLSYLQQFPIHTLKIDRSFVGDIRADHGDASIVNAIVAM
                     ARGLNLDLIAEGVENRTQLRYLRSQGCSEVQGFIFSDPVPADQVKLMLQQNPFRAMVL
                     DEQPEAATAV"
     gene            complement(11340..11927)
                     /locus_tag="AC16QF_15215"
     CDS             complement(11340..11927)
                     /locus_tag="AC16QF_15215"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6449959.1"
                     /translation="MSVDSELELRWERLGSEYGNDLILFKTRFDRMRNPRNDAVLRRL
                     VLESVDWVNIVALTADGLSVMVRQFRFGVDYTTLETPGGMVDPGEDSLAAARRELLEE
                     TGYGGGDWTYLGAVEPNPAFHDHLCHHWLASGVSLLQEPAPTPGEAIEVTLMSERAVA
                     DAVQQGVIKHALALSALSRVFELWPRPFRHERDPS"
     gene            complement(11924..13048)
                     /locus_tag="AC16QF_15220"
     CDS             complement(11924..13048)
                     /locus_tag="AC16QF_15220"
                     /inference="COORDINATES: protein motif:HMM:NF018805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HIR6449960.1"
                     /translation="MRIVTLPALLLTLLIVAPGCQPDEPAGGAMGEGTLRLGAVLGET
                     DAQGFARAEAVVPFAFPGDHGPHPRFRSEWWYLTANVQNEFGAEFGVQFTVFRQALAP
                     GPGPANPWLSNQVFLAHAAVTGVLEQGHWHEERVARGHPGVAGATAEPFRVWVDGWEL
                     AGRNGGFDEQRLSVKADAFAVDLQLDALKRPVLQGDRGLSAKGPEQASYYYSVPRLSA
                     RGTLRIGEVEHQVSGRAWIDREWSTSALSSGQVGWDWFALHLDDSWEIMVYQLRRADG
                     SRDRYDHAVLIDPYGAKHPLGADDFELEPEIFWQDDDGVAWPLQWRLKALDRELRIRA
                     AVPDQRMETSVSYWEGLVGVFDMAGRRTGRGYMELTGYEG"
     gene            complement(13055..15427)
                     /locus_tag="AC16QF_15225"
     CDS             complement(13055..15427)
                     /locus_tag="AC16QF_15225"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6449961.1"
                     /translation="MSLLGLSQLRFLLRARWSTLSVLVGLTLGVAAVVAVHQIGSRIN
                     ASLEMVGLPHLDGLTELLDRDGANLTDADYFALRARWRAGEYTDVRALVPMVEGQRVM
                     AGRRALVVGVDWIAASSLPASGSGIDGRALIADGRLLVDGSFGLRAGESIEVGGASFQ
                     VEGVLDADLGGLVLADLGIAQTLLGLPPQALSQIGVRRSDPYARASELAERVLPGISA
                     GFARRSSGWQPQGWRARSISAEQPSLGFARSVVFNVGALGLLVLVVAWFLIYQIAIIW
                     HRRRRLLNDRLEAMGVSRIELTRGFVRAFFLLGLVASFAGLLGGVVLARGLGAMTTLA
                     ADAAGQWPPITGWLLLKAGVSGMGVGSLSAWMACRREWGSGSGAPTWILAGVLLAVAA
                     AGVYFPVTGLFGGFAAVLALGMLVVAVVQPMLEALRASALRLRGRLLNRHALREVAWY
                     PRDLSVAVGALSLAVATSMAVGLMVESFRTDFAQMLDQRLAHDLYVDGGGRDLADVHG
                     WLLEQPSVDHVQPYGRARHRVGTVPIELGYSPLDEAEAARYGVRGGLGPGEALVSERL
                     AGLLGADVGDRLALGGGLRIAGVLSGFGEAGYRVLVDESVAADLGIELVHDRLSVSLA
                     ADDQVAGADLDARLLAAFPGVEVEPRGRMRQVAMEIFDRTFAITSSLTLLALLVAAIG
                     VHNSLVALRLNQQQGVHLLVMLGVTAGELARVAVRRALGIGSVAVALALPLGVLMAWL
                     LCAVINPRAFGWTVDLHLALSDVAVPLALGIGAAVLAGLLPSPRDSAGEI"
     gene            complement(15424..>15907)
                     /locus_tag="AC16QF_15230"
     CDS             complement(15424..>15907)
                     /locus_tag="AC16QF_15230"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6449962.1"
                     /translation="IELTTPEGTLRIDGLSDAARADVRRRQLGYVFQFFNLVPTLTVR
                     ENVLLPLELTGRGHLRAAALQRLADLGMDDRLDDFPENLSGGERQRTAIARALAHEPV
                     LVLADEPTGNLDGGNANLVTDLLWREVRAQGAALLLATHNEAVAARADHVLRLGAGAV
                     "
BASE COUNT         2873 a         5428 c         5057 g         2549 t
ORIGIN      
        1 tatgcggacc cgaaggtaag ggagacccgc gcccgcgcgg ttccgttggg ccgactgggc
       61 accgcagagg acgtggccaa cgcggtcgcc tttctcgact cggaggccgg cgcgtacgtc
      121 aacggccacg agctggtcgt ggacggcggc gtcacccaca ccctcctcaa gcacctgccg
      181 agggactgat gcgcaacgtg atgctcattg gcggcatcta tcatccgttc gaggaggcgg
      241 cacccgtgct cgcccgactc ctcgaatctc tcggcatcga gagcgtcatt accagcgacg
      301 tctcggaggc ggtggcggaa ctcgaatccg ccgacctctt caccatctac gccctgcgct
      361 ggcgcatgct caacgacgag aaatacgtgc ccttccgcaa gcagtgggca ttcgagctgc
      421 ccgaggaaga tgcccaggcc atagagaact tcgtggaagc cggcggcggg atgctcggac
      481 tgcacaccgc aagcatctgc ttcgacacct ggtacgagtt tccgaggctg ctcggcggct
      541 cctggcgctg gggcgagacg tttcatccgc ccgagggaaa cgtggaggtg cgcgcggtgg
      601 ccgatcatcc ggcgacgcgg ggcgtcaccg acttcggact ccacgacgag atctaccacc
      661 acctggccat cggcaacgaa agcaaggtac tgctcgaggg ccgggttccc gacggcgact
      721 ggcaccccat cagctgggcg cacgaggccg ccgcgggcag ggtcgtctac tcggcgctcg
      781 gccacgacgg acgatccctt ggcgaccctc atcatgcccg gttcatccgc caggcggtgg
      841 cctggctgct gggagaggac gagtgaggcc catcgccggc atgtcgatcc tcgtcaccgg
      901 cgccagctcc gggctcggcg ccggtgcggc ggcttacttc gccgagcggg gggccagggt
      961 caccatctcc gcccgccggg aggaccggct cgcggcggtg aaggaggcca tcggcgacgc
     1021 agccgccatg gtcgtcggcg acgtgaccag cgcagcggac cggcagcgca tgatcgacga
     1081 agcggtccgc cacggcgggg ggctcgacgc gctgatcagc aacgccggca acatgtaccg
     1141 cgggcccatc accgacctcg acgagcaggc gctcctggac atctttcaca gcaacgtcgt
     1201 cgccgggatg ctgctcacgg gcctcgccgt accggagctg gaaaagcgcc agggcgcggt
     1261 gatcttcgtc ggcagcatcc acaccatgag ggcattcccc ggcgcttcgc cctacgcggc
     1321 gacgaagggc gcgctgcaga ccctgaccag ggttctggcc gccgagctcg gcgagcgcgg
     1381 catccgggtg aattgcgtga tccccggcgc cgtgcccacc gagatctatc agcgtgcggg
     1441 cctgctgtcg gacgaggacg cgcgggctcg catcgcgggg atggcctcca tgcaccccct
     1501 cggccgggtc ggcaccgagg aggagctcgc cgaggccatg gcttacctga ttcaggcgga
     1561 gtggaccacg ggggccgagc tggtggtgga cggcgggctg gggctagggg ccaccaacgc
     1621 gtagcgggca gtggtggcgt atcgtcgccg gtgacttaaa atgtccgctg gggataagga
     1681 caaatcaggg ggaggcgatg gccgagagcc agcccaagcc gggtgaggca cgctgcccgg
     1741 gcatttcggt acaggacctg atggacaggg acagccgaac ggtgcccgcc tatctgcgcg
     1801 agcacagcta cgcgtttctc ggcgacgagg acattccttt tgcccgctac acgtcgcgcg
     1861 actggttcga caaagagatc gagcacatgt ggccgagaac gtggcagtgg gcgtgtcgcg
     1921 aggagcacct gccggccccc ggtgattatt acgtttacga cgtcggcccg tactcggtca
     1981 tcgtgttgcg ggacagcagc ggcgcgatcc gggcataccg caacgcctgc atgcatcgcg
     2041 ggacgaagct caagccctcg ttcagcacgg gttcggctga ctccatccgc tgcccttatc
     2101 acggctggca gtggaacctg gagggcgagc tgcaatcgtt tccctgcgag tgggatttcc
     2161 cccacgtcga tcccgatgcc gtgggcctgt cctccgtccg ggccgaactc tggggcggct
     2221 tcgtgttcat caacctggat cccgaggccg ttccgctacg ggagtacctt catcccctgc
     2281 ccgagcacgt ggacagcagc gtcttcgagc aacgctacgt ggccatccac atacagaaag
     2341 agctgaactg caactggaag atcgcctcgg aggcgttttt agaagcctat cacgtgatgg
     2401 aaacccaccc tcagctcatg cgcaccaacg gcgacgcgaa cacccagtac gacttctacg
     2461 gcgatcacgt caaccggctg atcaacgtcg ccggctcgcc gagcgcttac ctcgaggagc
     2521 cgataaccga gcaggaggtg ctggatctgt ttctggtggg ggatcgtgag gatatcggcg
     2581 aggcgctggt ggtgcccgag ggcggcaccg ctcgccaggt catggccgag catttcagag
     2641 agagcgtgaa cgcgtccggg ggagcggatc tctccgaagt gtgcgattcg gagatcgtgg
     2701 acaccatcgc ctacttcgtc ttccccaacc agcagttctt ctccggggta tcgtttccca
     2761 tcgtttaccg cttccggccg ctcggcatga gccacgaccg cagcctgttc gacttcgtca
     2821 tcctcaaaac gcggcccaac gacggctctc cagcacccac ggccgagccc gtgcggttga
     2881 cggcggacga gtcgtacacg ctggtaccgg gcatggatcc ctatgtcggc cacgttttcg
     2941 atcaggacac cggcaacgtc caggcagctc aggagggtgc catggccacc gacaaggccg
     3001 gcgcgaccct gggcaattat caggaaatac gcatccgtca ctttcaccag actctcggca
     3061 agtacgttgc tggcgagctt taggagcaca cgatgacaga cgttctgatc accggggcag
     3121 cccgcggtat cggtttcgag ctggccaggc aatacgcgga ggccggggac cgggtctacg
     3181 ccaccagccg taatcccgac ggcgcagccc aactcagcgc gctggcggcc gatttcccgg
     3241 acaacgtcag cgtgcaccga atggacgtgg gcgacatggg gtcggtgcag ggctgcgccg
     3301 ccgagctgaa cgcagcgccc atcgacgtgc tgatcaacaa cgccggcgtc tggggcgggc
     3361 tggagaccca gacttttcag aacatggact acgagaactg ggcgttcgag ttcaacgtca
     3421 tggccatggg ccccttccgg gtcatccaga cctttctgcc gaacgtgctc gccgggcgcg
     3481 acaagaagat cgtgacgctc tccagccagg tagccgccca cgtctacgat cacgtcatcg
     3541 gctactccta cgccagcgcc aaggccgccg tgaaccggct gatgacggcc ctggccaccg
     3601 agctccagga ccagggtatc atcgtcgttc ccatgcaccc ggggtgggtg aaaaccgaca
     3661 tggcgggcga cgtcgcagac atctatcctt cggaaagcgc cgagggcgtt cgccgggtca
     3721 tcgccgggat gaccatgaag gacacgggca agttcctgaa atggaccggc gacattcacc
     3781 cgtggtagcg acacgaacgg tttctgccgg gcgaccggca cacacgcaat gcaaacagat
     3841 cagggaaggc taaaccaatg aatacaggct acaacccgga gttcaatccc accggaatcg
     3901 aaggcggcat cgacaccaac gcgcttccct ggatcgacct gccccaggca cccggggtat
     3961 cgatcaagcc gctccgggcc agcagcgagt cgggcatgtt ttccaccgtg gtcagggtag
     4021 ccgatggcag cgagctgtcg agcatcgtct acctcggcgc catggacatg atcgtcctgt
     4081 cgggccggct cacctacccc tcgggaccca tgtcggggac cctggagccc ggaacctggg
     4141 gctacgtgcc cgcgaatgcc cgggtggaac ggttcattgc ggagcaagac acggagtttc
     4201 tggcgaactt ctacggtccg gttgcctttc tcgcggacga cgggcgcacg gtgtcgagcc
     4261 tgctgaccag cctggacatc caggcggccg cgcgcgaccg gggcatcaac ctggtgccca
     4321 acaccctggc ggagtgcatg caggagcggc cgcccgctta tcagggcccc gtggagccac
     4381 tggccatcgc cggcagcgac tcaaaggcac tggtggttgc cgccgagggc gtcgctgcag
     4441 atgcgggccg tgcggtgcac ccgcacttca tcgacacccg agcggttccc tggatcgtca
     4501 atccggacac gcccgacctg ggcctgaaga tactccgggt cagcgaggaa accggcatca
     4561 cgtcgctcat cgtgcggcac aacggcgtcg ccgggcccca ctaccacctg ggcgccgccg
     4621 acttcatggt gctcagcggt cacatcggtt atcgggcagg accgcccgag ggctacgggc
     4681 cgggggtctg gttctacgag cccgccggag cgcgtcacga agccacccag cgcatcggcg
     4741 aggaagatct catctacacc gcaaacgtct acggcccgct gcagttcgac gagggccccg
     4801 acacgcccat cgcggccgtg ctgtcctgga tgcagtacaa ggcaatggcc gatgccggcg
     4861 gggtagatct cgtgcacagc accttcgaca acgacgcctc gctgctcgcc tgggcgccca
     4921 tcggcagcga gggcgcccgg ctctgagccg ggcttcggcg gaagcaagcg ggggatcgac
     4981 gtgcggatgg acgtgaacaa aggcatccgg gtctgcgagg aaatcgcagc ctcggaaacc
     5041 gaccccagga agcgccacaa tctggagatg gtgattgccc acatgcgggc ggaagctcgc
     5101 tgtgacgtcc agggcgttct cgacacgctc gtcgacgagc catgctacgt ctggcacagc
     5161 gcgcccgacg atccgttgct caacccacgc ggcagcaggg aggcggtcgc cgacttctac
     5221 gatcggatga tcgtcaggac cggcgcccat cggctcgaat gggatatcac ccgggtcatg
     5281 gtggacgagc acgccgtgct caccgaaggc gtcatgcggg tcgcgtttcc cggctgggcg
     5341 ctggcggaga tggacatcgc cgtcgacgac ccggacgcct actacgtggc ggagggcatc
     5401 accacggtca tctggcccgt cgacccggaa accgggctgc tcgtcggaga agaggtctac
     5461 gagtacgaca agatggccgg gatcgccgat cggaagatcg cgccgagcga catcctgccg
     5521 ctgtcctcgg cggcgtaacg tccgggcaat gacacgcatg gcaccatgac cgatcccgtc
     5581 acggaactgc gcgatgccat cgatcggcag accatcgccg atctgcagaa ccgctacgcc
     5641 tatgccatcg acgccggtcg ctacgacgac ctggacgggg tgttcactcc cgatgccgtg
     5701 gccgactacg gccccgcggg acgggtcgag ggcgtggaag cgatcaaggc gacctgccgc
     5761 cacgctttag agccgctgac ggccgcgcag cacatcaaca ccaaccactg ggccgtcatc
     5821 gaaggtgacc acgcgcgggc cgggtgctac ctgcacgtcc atcagcaccg ggagagcacg
     5881 ccgggcggcg atcatctgga gatgggcggc cgctacgagg acgcgctgat caggacgccg
     5941 gacggctggc gcatcacctc gcgcaagctg accatcctct ggagccaggg caaccctgag
     6001 gtgcgctggg agcgctagag atcgagcgtc gagcccgtga gacgctggta ggcttcgaga
     6061 tagcgcccga tgctcttctc cacgacctca tcgggcaacg cgggtccagg gggcgttttg
     6121 tcccactccc ccgccgccac caccgtctcc aggtaattgc ggacgatctg cttgtcgaag
     6181 ctgggctgct cgcgtccggg ttcccactcg tcggcaggcc agaacctcga gctgtcgggc
     6241 gtgaatatct cgtcgatcag aatcggttcc tcccgacccg gcagctgacc gaactcgaat
     6301 ttcgtgtcgg cgagaatgat gccgcgctca agcgcgtagt cgcgcgcctt ggcgtacaac
     6361 ctcagggtcg tgtcgccgag ccagtccatg agctcgcggc cgacgatgcc gacgccttcc
     6421 tcgaagctga tgttctcgtc gtgaccggtg tccgccttgg tggatggcgt gaacagcggc
     6481 tcttccagcc gatcgctgtt tctcaacccc tcgggcagct cgattccgca caccgcaccc
     6541 gtgcgctgat aatccttcca gccgctgccg gtgatgtagc cgcgcgcgat gcattcgatg
     6601 gggaccacgc gggtcttgcg gcacagcatg atgcgtcctg cgagggcctc tctctgggcg
     6661 tcgctcagcc cgggcacgtc tgccacgtcg gtggaaatca gatggtggtc gacctctccg
     6721 gcgaagtggt cgaaccagaa ccgtgagatc tgggtgagaa cgatgccttt gccgggcagg
     6781 ccgttggcca tcacgacgtc gaacgcgctg atccgatcgg tggcgatgat cagcagagcc
     6841 tcctcgccgc cgtcgagggt gacgtcgtag agatcgcgaa ccttgcctga gcgtcggttc
     6901 ggcaaaccga gattcgtttc cagtagcgcc tgtctcattt ttcccccatg tccaatcgag
     6961 tcaaactttc aatgtcaggc cgtcatcccg ccgtccgcca tgtagaccga gccggtgacc
     7021 caagcgctgt cgtcgctggc aagaaagagc atgaccttgg cgatctcctc cggctcggcg
     7081 tatcgctgca gcgcgatgcg cgactgcatc aggctcttca cctgctcgcc gccccccggc
     7141 gcgatgccct cctcgatgct tcgcatcata cgcgtctcca cgggagatgg attcacagtg
     7201 ttgacgcgga tgttctgccc ggcgcattcc cgggcggccg atcgcatcag cccgatcacc
     7261 gcgtgcttgc tggtgacgta agcggacatg cccggagatc ctccgacgcc ggcaaccgag
     7321 gaggtgatga cgatgctgcc gccgccacgc cgcgccatgg cggggatggc cgatttcagc
     7381 cccaggaaga cgcccttgac gttgacgccc aggacctgat cgaacctcgc ctcgtcgtag
     7441 tccacgatgg gcacggcaac gtcgccttcg atccccgcat tggcaatgag cacgtccacc
     7501 ccgccgtagc gctcttccgc gcacgcgacc atggcggcgt tgtccgcaga agcggtgacg
     7561 tcaccgacaa agtagctgac gcggttgctg ccgataccgc gcacggtttc ctccagcgcc
     7621 ttctcgtcca catcgaccag caggacgtcg gcgccttcct cggcgaaaag ccgacccgca
     7681 gcagcaccga tgccaccggc gccccccgtg atgacggcca ctttaccctt cagtcttccc
     7741 atgccttgcg cttcccccgc acaccgattc agcccagcgc gcgctcgaga tcccccagga
     7801 gatcctcgct ctcctcgatg cccacggata tgcggatcag cgaatcgtct atgcccatgc
     7861 tcgcccgccg ctccggaccc atctcgtagt aaatcgtgtg agccacggga atcgccaggg
     7921 tccggttgtc acccaggttg ctggacttga tgatcacatc caacgcgttc atgaagttcc
     7981 agatgtccac ctcgggaacc agctcgaagc tgaacagcgc gcccggatgg cggaacaggc
     8041 ggctcgcccg ctcgtgctgg ggatgctgct taaggccggg atagtagacg gctttgacct
     8101 tcgggtgcgc atcgagcatc gccgtcatgg cggccgcgtt ggcacactgg cgctccagcc
     8161 gcaacccaag ggtctccgag cccacggcga tgtgatgagc ggcttccggg gcaagcgcgg
     8221 cgccgaagtc ccgcagtcct ttcttcttga tctgggtgat ggcccacttc tcggcggccc
     8281 cggatttgta cgtatcgtag atgttggaaa aatccgccca gtcgaaattc ccgagctccg
     8341 tcaccgctcc gcccagcgcg ttgccgtgac cgccgatgta cttggtcagg ctgttcacca
     8401 ccaggctggc gccgacgctg gccggcagaa acaggtaggg cgacgtcatg gtgttgtcca
     8461 ccacgtaaat gaggccgcgc tcggcgcaca gcttaccgat ctcctccaac gcagatacct
     8521 gcgtgcgcgg gttggcaatg gtttccacga acacgagccg ggtacgctcg ttgatgccgg
     8581 cggccacggc gtccacatcg gtggcatcga cgaacgtgac gtccaccccg tgggtcgaaa
     8641 aggagttgaa caggctgttg gtgttgccga acaggaagga gctcgacacg aagtgatcgc
     8701 cgttgcgaag cagggcgaag agcatggtcc cgatggcagc catgcccgtg gagaaacaga
     8761 cggtctgcag cccctgttcc atggtggaga tcttggtctg gagggcttcc acggtggggt
     8821 tcacctgccg gccataggtg tagccggctt tggtgccctg gaacaccgcc gcaaggtcgc
     8881 gggcgtcgtc gtacccgtat gccaccgtgg agtgcaccgg cttgtgcagc gagccgtgct
     8941 cgatgacctc gagccggtcg ctgtgcacga tcttcgtggt gaaacccttc atcggtagca
     9001 gcgcgccgtg ctagacggct gtggcggctt ctggctgctc gtccaggacc atggcccgga
     9061 acggattctg ctggagcatc agcttgacct gatctgccgg caccgggtca ctgaatatga
     9121 agccctgcac ctcgctgcag ccctgggaac gcagatagcg aagctgagtg cggttctcca
     9181 cgccctcggc aatgaggtcc aggttgagac cccgcgccat ggcgacgatg gcgttgacga
     9241 tggaggcatc gccatggtct gcgcggatgt cgcccacgaa gctgcggtcg atcttcagcg
     9301 tgtggatggg gaactgctgc aggtagctca gcgacgagta tccggtgccg aaatcgtcga
     9361 tggcgatccg caccccgagc tgccgcagct gcttgagctt gggaatgacc acctccatgt
     9421 cctgcatcag gatgttctcg gtgatctcca gcttgaccct ggagggatcc aggccggcag
     9481 cagccatgtc cgcaacgaaa ttatcgacga agtcctcctg ctcgatctgc ggggcagaca
     9541 ggttcacgga cagccggata cttccaagcc ccgcgcgctc ccagccggca acatcggtgc
     9601 acgcccggcg ctgaacgaag gtgtcgatct gggtgatcag accggtttct tcggccatgg
     9661 gcaggaagtc gccgggctct accagcccgc gctccggatg ccgccaccgg acgagcgcct
     9721 cgacaccgac gatctccccg gtcttcagcg ccacctgagg ctggtagtac acgaccagct
     9781 cttcctgggt aagcccgttg cgaagctcgc gctccatgga cagccgggtc gagtacttgt
     9841 ggttcatctc ctcggagaag aactggtagc cgttcttgcc gcgacccttg acgtggtaca
     9901 tggcaatgtc ggcgttctgg atcaacgctt cctcggtctc cccggcctcc gggtacatgg
     9961 ctacgccgat gctggcaccc acgaacagct cgaggccatc aatgacgaag ggaccgccca
    10021 gccggtcgag gatcttggtg gcgatgacca ccacgtcgtc gcgcgtgcgc acctccggca
    10081 gcagcagcgt gaactcgtcg ccgccgaagc gcgacagcgt gtcgccgcgc cgcagacagc
    10141 tctgcaggcg attggctacc gctttgagaa gccgatcgcc catgctgtgg cccagggtgt
    10201 cattgacgat cttgaagcgg tccagatcca ggaacatcac ggccagtctt cgcttgctcc
    10261 gccgtgcctg ggtgattgcc agcgacaggc ggtccttgag cagagcgcgg ttcggcagat
    10321 gggtgagcag atcgtggtag gcctgaaagc tgatgacctc ctgggcctct ttgcgttcgc
    10381 tgatatcccg cgcgatgccg taggttccaa cgaagttttc ccgggtacgc tccttgggat
    10441 cggaataaac gcccatggcc gtgagctcag tccagatggc gtgggcatga aacagacgcg
    10501 cgccccgtcg ggcgtggcgg ctcttcaggc gcatctccgc gttggacacc gccctgtcgc
    10561 ccgtgcgccg ctcgttgaac acgttccgcg caagctccag gtggtcgtcg tccaccagct
    10621 ccgtgtagtg cttgccgatg agttcgtcac gggagaatcc cagcagactt tcgacacggt
    10681 cgttgagaaa ggtgaagcag ccgttgcggt cgagcatgta gaccagatcc ggggagctgt
    10741 tgacgatgta gcgatgcagc tcctctgatt ccttgacctt ctcttccatg agctcgtttt
    10801 gagcctcgag ctggcactgt cgcagcgccg tctccatggt cgcgatgagc tcgcccgcct
    10861 catagggctt cttgacgaag tcgaaggccc cgcaatgcag cgcgtgcttg acgccgctga
    10921 agctggaatc gccgctgacg acgatgattt tgcaggtgag ctcgttgcgg gccgcgtagt
    10981 cgaggacgtc gtgcccgctc acgcccggca tgatcaggtc cagcagaacg acgtcgtagc
    11041 ttttctccgc cagaagattt agcgcctgct ggccgccgag ggctttgtcc ggctcgtagc
    11101 cgtgcagcac catcagctgg taaaggctgt tgagcagctc gggtttgtca tcgacgagga
    11161 gaatcgacgc acccaccagc ccggacccgg cgctttttcg attgagcaat cgggagaatg
    11221 actctcgggg actggaagcg tccgccgtgc tgctttcact cataccagaa ctgtcccacg
    11281 tcgggctcga catgcttacc aattgtgacg aatttttgct gctctggata ggcgcccgct
    11341 catgacggat cacgctcgtg ccgaaacggg cgcggccaga gctcgaacac gcgcgacagc
    11401 gcggaaagcg ccagcgcgtg cttgatcacc ccttgctgca ccgcgtcggc gaccgcccgc
    11461 tcgctcatca gcgtcacctc gatggcttcg cccggggtcg gcgccggctc ctgcagcaac
    11521 gaaacgccgg aggccagcca gtgatggcag aggtggtcgt gaaaagcggg attcggctca
    11581 acggcgccga ggtaggtcca atccccaccg ccgtaaccgg tctcctccag gagctcccgc
    11641 cgggcggcgg cgagcgagtc ctcgcccggg tccaccatgc ctcccggagt ctccaaagtc
    11701 gtgtaatcca cgccgaagcg gaactggcga accatgacgg agagcccatc ggccgtaagc
    11761 gctacgatgt tcacccagtc cacggactcc agaaccagcc gccggagcac cgcatcgttg
    11821 cgcggattgc gcatcctgtc gaagcgggtc ttgaacagaa tcaggtcatt gccgtactcc
    11881 gagcccagac gctcccagcg cagttccagc tccgaatcca cactcatcct tcgtaccccg
    11941 tcaattccat gtagccgcgg ccagtgcgtc tgcccgccat gtcgaagacg cccaccagcc
    12001 cttcccagta ggaaacgctg gtctccatgc gctgatcggg aacggcggcg cggatgcgca
    12061 gctcgcgatc gagcgccttc aggcgccact gcagcggcca ggcaacgccg tcgtcgtcct
    12121 gccagaagat ctccggctcg agctcgaagt cgtcggcgcc aagaggatgc ttcgcgccgt
    12181 aggggtcgat gagcacggcg tgatcgtagc gatcccggga cccatccgca cgcctgagct
    12241 ggtagaccat gatctcccag ctgtcgtcga ggtgaagggc gaaccagtcc caacccacct
    12301 gcccggacga gagcgcgctg gtgctccact cgcggtcgat ccaggcccgg ccgctgacct
    12361 gatgctctac ctcaccgatc ctgagcgtgc cccgcgcact tagccgcggc accgagtagt
    12421 aataggaagc ctgctccggc cccttggcgc tcaggccgcg atcaccctgc agaacgggcc
    12481 ttttcagcgc atccagctgc aggtcgacgg cgaacgcgtc agccttcacg ctcaacctct
    12541 gctcgtcgaa gccgccgttc cggccggcaa gctcccagcc gtccacccag acgcgaaatg
    12601 gctctgcggt ggcaccggcg acgcccgggt ggccacgcgc cacgcgctcc tcatgccagt
    12661 gcccctgctc caggacgccg gtaaccgctg cgtgcgccag aaagacctgg ttggacagcc
    12721 acgggttggc tggcccgggc ccgggcgcca gcgcctggcg aaacacggtg aactgcaccc
    12781 cgaactccgc gccgaactcg ttctggacgt tggcggtcag gtaccaccac tcactcctga
    12841 acctcgggtg agggccgtgg tccccaggga acgcgaacgg cacgacagcc tccgcccggg
    12901 caaaaccctg cgcgtccgtc tcgccaagca cggcgccgag ccgcagagtc ccttccccca
    12961 tggcgccgcc cgccggctcg tccggttggc aaccgggggc cacgatcagc agcgtcagca
    13021 gcaacgcggg cagggttacg atgcgcattc cctatcagat ctccccggcc gaatcccgcg
    13081 gcgacggcaa cagtcccgcc agcacggcag caccgatccc gagcgcgagt ggcacggcca
    13141 cgtcgctcaa tgccagatgc agatccaccg tccagccgaa ggcccgcggg ttgatcaccg
    13201 cgcacaacag ccatgccatg agcaccccca ggggcagggc caacgctacc gcgacgctgc
    13261 cgatgcccag cgcccggcgc acggccaccc gggcgagctc gcccgcggtg acgcccagca
    13321 tgacgagcag gtgcacgccc tgctgctgat tgaggcgcag ggcgacgagg gagttgtgca
    13381 cgccgatggc ggccaccagc agggcgagca gggtcaggga ggacgtaatg gcaaacgtgc
    13441 gatcgaagat ctccatcgcc acctgacgca tccgcccgcg gggctccacc tccacgccgg
    13501 ggaaggccgc aaggagacgc gcgtcgagat cggcaccagc gacctgatca tccgccgcca
    13561 gcgagacgct gagccggtcg tgcacgagct cgatgcccag gtcggccgca accgactcat
    13621 ccacgagcac acggtaaccg gcctcgccaa aaccgcttaa aacgcccgcg atccggaggc
    13681 ccccgcccag agccagacga tcgcccacat ctgctccgag caagccggcc agccgctcgc
    13741 tgaccagcgc ttcccctggg cccagccctc cgcgaacgcc gtatcgggcc gcctccgcct
    13801 cgtcgagcgg cgagtagcca agctcgatgg gcacggtgcc gacgcgatgt ctggccctcc
    13861 cgtagggctg cacgtgatcg accgacggct gctctaagag ccaaccgtgc acgtcggcca
    13921 agtcgcgccc gcccccatcc acataaagat cgtgggcgag gcgctgatcg agcatctgcg
    13981 cgaagtccgt gcggaagctc tccaccatca gccccacggc catgctagtg gccacggcca
    14041 gcgacagcgc accaacggcc acggacagat cccgcgggta ccacgccacc tcgcgcagag
    14101 cgtgccggtt gaggagacgg ccacgcaagc gcagcgcaga ggcccgcaac gcctccagca
    14161 tgggctggac gacggcaacc accagcattc ccagcgcgag cacggctgcg aagccgccga
    14221 acagacccgt caccgggaag tagacgccgg ccgcggcgac cgcgagcagc acgccagcca
    14281 ggatccaggt gggcgccccg gagcccgatc cccactcgcg gcggcaggcc atccacgcgc
    14341 tcagcgagcc aacgcccatg cccgacacgc cggctttgag caggagccaa cccgtaatcg
    14401 gcggccactg cccggccgcg tctgcagcca gggtcgtcat ggcaccgagc ccgcgggcca
    14461 gcacaacgcc gccgagcaac ccggcgaagc tcgccaccaa gcccagcagg aaaaacgcgc
    14521 gcacgaaccc acgggtcagc tcgatcctgg acacgcccat ggcttccagc cggtcgttga
    14581 gcaggcgacg gcgacggtgc cagatgatgg ctatctggta gatcagaaac cacgccacca
    14641 ccagaaccag cagacccagc gctcccacgt tgaaaaccac cgatcgcgca aagcccaggg
    14701 acggctgctc cgccgagatc gagcgagcgc gccatccctg cggctgccac cccgacgagc
    14761 ggcgggcaaa ccccgcggag attcccggga gcacgcgctc ggccagctcg ctggcccgcg
    14821 cgtaggggtc cgatcggcgg acaccgatct ggctcagcgc ctggggaggc aatcccagca
    14881 gcgtctgggc gatgcccaga tcggcaagca cgagcccgcc gaggtcggcg tcgagcacgc
    14941 cctccacctg gaatgacgcg ccgcccacct cgatggactc gcccgcccga aggccgaagc
    15001 tcccgtctac cagcaggcgg ccgtcggcga tcagcgcccg accatcgatc ccggaaccgg
    15061 aggccggcaa actcgaggcc gcgatccagt cgacgcccac cacgagcgcg cggcgtcccg
    15121 ccatcacccg ctgaccttcc accatgggaa ccagcgcccg cacgtccgta tactcgccag
    15181 cgcgccagcg cgcccgaagc gcgaaataat cggcatcggt gagatttgcg ccatcgcgat
    15241 cgagaagctc ggtgaggccg tctagatggg gcaggccgac catttccagg gaggcgttga
    15301 tgcgggaacc gatctgatgg acggccacga ccgcggcaac gcccagggtc agccccacca
    15361 gcaccgaaag cgtgctccac cgcgcccgca gcagaaaccg cagctggctg agcccgagca
    15421 ggctcacacc gcgcccgcgc ccagccgcag gacgtggtcc gcgcgcgccg cgaccgcctc
    15481 gttgtgtgtc gccaacagca gggcggcgcc ctgggcgcgt acctcgcgcc agagaaggtc
    15541 cgtcaccaga ttcgcgttgc cgccgtccag gttgcccgtc ggctcatccg ccagaaccag
    15601 caccggctcg tgggcgagcg cccgggcgat ggccgtgcgt tgacgctcac cgcctgagag
    15661 gttctccggg aaatcgtcga ggcggtcgtc catccccaga tcggccagcc gctgcagggc
    15721 ggccgcccgc agatggccgc gcccggtcag ctcgagcggc agcagcacgt tttcccgaac
    15781 cgtgagcgtc ggcacgagat tgaagaactg aaacacgtat ccgagctgcc ggcgtctgac
    15841 gtccgcacgg gccgcatcgc taagcccgtc gatccgcaga gtaccttccg gggtggtcag
    15901 ctcgatc
//
LOCUS       DBNHLM010000329         7998 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_109999, whole genome shotgun sequence.
ACCESSION   DBNHLM010000329 DBNHLM010000000
VERSION     DBNHLM010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_109999"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..312
                     /gene="rpsT"
                     /locus_tag="AC16QF_15235"
     CDS             46..312
                     /gene="rpsT"
                     /locus_tag="AC16QF_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011336008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR6449963.1"
                     /translation="MANSPQARKRARQAEKRRRQNASQRSMVRTYIKRVDAAIQSREY
                     DAASAALNDAIPVIDRMASKGIMHKNKAARHKSRLNAKVAALKA"
     gene            complement(313..1449)
                     /gene="proB"
                     /locus_tag="AC16QF_15240"
     CDS             complement(313..1449)
                     /gene="proB"
                     /locus_tag="AC16QF_15240"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR6449964.1"
                     /translation="MRARLAAARRVVVKIGSALLTDDGAGLATSAMGSWCEQVNRLMD
                     AGVQVLIVSSGAVAEGVSRLGWSSRPVGVHEQQAAAAVGQMGLMQAYERAFAGHARHT
                     AMVLLTHDDLADRERYLNARATLTRLLDLGVIPIVNENDTVATDEIRFGDNDTLAALV
                     TNLLEADLLVILTDTDGLHERDPRKDSQAPLVSRADAADPRLDVMAGSTSGALGRGGM
                     VTKIQAARIAARSGAHTVIPSGSIEGVLNRVLEGADVGTLLLASMNPLDARKRWIAGQ
                     LKVKGELELDRGAVEALRRRGVSLLPVGVSAVRGRFVRGDVVRCVDGDGLAIAQGLVN
                     YSSEEAARLLRAGSDEIADRLGYAAEPELVHRDNLVLLSGGSKP"
     gene            complement(1473..2663)
                     /gene="cgtA"
                     /locus_tag="AC16QF_15245"
     CDS             complement(1473..2663)
                     /gene="cgtA"
                     /locus_tag="AC16QF_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016713347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HIR6449965.1"
                     /translation="MQFIDEATIRVEAGKGGGGCLSFRREKYVARGGPDGGDGGDGGD
                     VILVADDSLNTLVDFRYQPLYRAGNGQPGSGRNKTGAHGDARLVRIPVGTSVIDDETL
                     ETLGDLTEAGQELLVARGGRRGLGNAHFKSSVNRAPRRTTTGEPGEVRRLRLQLKLLA
                     DVGLLGLPNAGKSTLIAQVSAARPKVADYPFTTLVPSLGVVGAGTDNSFVMADIPGLI
                     QGAAEGSGLGVQFLRHLARTRILLHLVEVAPLDGSDPIDNARLIEAELADYSQALRER
                     PVWLVLSKTDLVTDADLEGLERRFADAFPGRPLFAVSAVTTAGIGRLTDALLAAVAGI
                     RERLAGDEEYAEREAELQARIGEDVLRGAVLAKPGALRGNSEERDEADDDGEDQVQVT
                     YVPE"
     gene            complement(2712..2981)
                     /gene="rpmA"
                     /locus_tag="AC16QF_15250"
     CDS             complement(2712..2981)
                     /gene="rpmA"
                     /locus_tag="AC16QF_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020291029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR6449966.1"
                     /translation="MAHKKAGGSTRNGRDSESKRLGVKKYGGQFVAAGNIIVRQRGTK
                     FHPGDNVGCGRDHTLFATADGYVQFLTRGPHKRKFVCVEPATAEA"
     gene            complement(2999..3310)
                     /gene="rplU"
                     /locus_tag="AC16QF_15255"
     CDS             complement(2999..3310)
                     /gene="rplU"
                     /locus_tag="AC16QF_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR6449967.1"
                     /translation="MFAVFSSGGKQHRVTEGEVIRVERLAGEPGDEVVFDKVLMVADG
                     DDVSVGQPFVDGGKVTAEVVSSGRGKKIRVIKFKRRKDYMRRQGHRQWYTELKITGIN
                     G"
     gene            3501..4526
                     /locus_tag="AC16QF_15260"
     CDS             3501..4526
                     /locus_tag="AC16QF_15260"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR6449968.1"
                     /translation="MPGDPAIATTTTRISADPNDALTPVYDLARDDFAEVNRLIPHQL
                     TSDVELVEEIGRYIVDSGGKRLRPLMVLLGARCCGYEGDDHVRLAAIIEFLHTATLLH
                     DDVVDRSELRRGRATANALWGNAPSVLVGDFLYSRAFQLMVELEDMDIMAILADATNT
                     IAAGEVQQFASIGNFAIDEAQYMEIIRCKTALLFQAATHTAGVLSSEDPDEVAALKNF
                     GLHFGLAYQLVDDWLDYEGDSSTLGKNVGDDLAEGKLTLPLIHALANAPDADANVVRD
                     AVSARSAERLAEVLQVVRSSGALSYTRSAATRQCARAVECLTGLPDNEYRDALETLAN
                     FCLDRLR"
     gene            4529..5986
                     /locus_tag="AC16QF_15265"
     CDS             4529..5986
                     /locus_tag="AC16QF_15265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6449969.1"
                     /translation="MRPAPSILLACLCFLPFASALAAAPALEQCELSGSHGVGRVEAV
                     CGRLPRPENPEQPDGARLDLFIAKIPSLSPEPAPDAFTIINGGPGASSVSLFVDLAQA
                     FSGVLRERDIVLVDQRGTGRSNALECPELEKAAEDYSIEAVQEATRACLESLAGDPRF
                     YTTSQAVQDLEAVRAALGYERLNVYGVSYGTRVSMHYLRRYPDRVRTLILDGVVPPGL
                     ALGVNVAENAQRRLDELFAQCDRAPECSEAFPDLQASFTQLQSNLNAEPASLRFPNPI
                     TGLEESLAFGYDHLALVVRLLSYAPETASLLPLIIDQAAGGNYLPAASQALRITDALS
                     GTMSFGMHNSVTCTEDLPFIDFSQVNWDALEATYIGADQVRALRAICEIWPRGAMDPD
                     LKDPVQADTPTLILSGELDPITPPAYGDQIKPGFGNSMHIIGAGQGHGVVSRGCLPGL
                     VTQMVEAGHLGDLDAGCVERLSAAPFFIDLMGPAP"
     gene            5983..6708
                     /locus_tag="AC16QF_15270"
     CDS             5983..6708
                     /locus_tag="AC16QF_15270"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6449970.1"
                     /translation="MIRAEGLDKSFGEVTAIRHVSFDAADGKVTALLGPNGAGKSTSL
                     RILATVLQPDGGKAEVDGVDVVADPMAVRRQIGVLPHAAGLYARLTARENVAYFGSLH
                     GIAKSRLSERIDELIELLEMGEFAERRVAGFSQGQRIKVALARALVHDPRNVLLDEPT
                     NGLDVMATRALRDIIIRLRDEGRCVLFSSHIMQEVANVCDDVIVIGHGRVLFQGTLDG
                     LRDHAGEADLEEAFIRVIDEEDV"
     gene            6705..7877
                     /locus_tag="AC16QF_15275"
     CDS             6705..7877
                     /locus_tag="AC16QF_15275"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6449971.1"
                     /translation="MTGFAIVLAKECRDNIRDRRTIISSLSLAVLGPALFVGLMAFVL
                     DSAVGESSDPVEITVVGADHAPGLMAFLETQNTRIERVDLEDPRGAVAGGSRKLVLSI
                     PDDYSERFAAGQVTTVSLIYDSSDMGMSRRNFRMAQQFILAHARMIGLLRLQLRGVDP
                     AIASPVQVQAVDTASPAARALAILATLPYLLVLVIFMGGFYLAIDATAGEREHGSLEP
                     LLTQPISRGALVLGKVAATAVFSAASLALFLASLAISLPYAPLHKVGMSLHIDVSTAL
                     TVFVMALPLILFAAALLNVVASFAKSYKEAQTYLTFVILIPTLPLIITRMMNVEPSAA
                     LMMIPSLSQGTLINDAVAGEPVQWVFLLISVVSTTLTAGALTMLAVYLYKRERILI"
     gene            7940..7998
                     /locus_tag="AC16QF_15280"
     tRNA            7940..7998
                     /locus_tag="AC16QF_15280"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7966..7968,aa:Pro,seq:cgg)
BASE COUNT         1349 a         2789 c         2465 g         1395 t
ORIGIN      
        1 ggcatattac cgcgccttcg caatccggac ccccaggagc cacgcttggc caacagtcca
       61 caagcacgca agcgcgcacg ccaggccgag aaacggcggc gacaaaatgc cagccagcgt
      121 tccatggtgc gcacctacat caagcgcgtc gacgcggcca tccagagccg ggagtacgac
      181 gcggccagcg cagccctgaa cgatgcgata cccgtcatcg accggatggc ttccaagggc
      241 atcatgcaca agaacaaggc agcccggcac aagtcgcgcc tgaacgccaa agtcgccgcc
      301 ctgaaagcct gatcagggct tcgacccacc ggagagcagc accagattgt cccggtgcac
      361 gagttccggc tccgccgcat accccaacct gtcggcaatc tcgtcagacc cggcccgcag
      421 caatcgggca gcctcctcgc tgctgtaatt gaccagcccc tgagcgatcg ccagcccgtc
      481 gccgtccacg cagcggacca cgtcaccgcg cacgaaccgt ccccgcacgg cagacacccc
      541 tacgggcaga aggctgaccc cgcggcggcg cagcgcctcg accgcgcccc tgtcgagctc
      601 cagctcgccc ttcaccttga gctgaccggc gatccacctt ttgcgcgcat cgagcgggtt
      661 catcgaagcg agcagcagcg tgcccacatc ggccccttcg aggacgcggt tcagcacgcc
      721 ctcgatactg ccgctgggaa tcacggtgtg ggccccggag cgtgcggcaa tgcgggccgc
      781 ctggatcttg gtcaccatgc cgcctcgccc caacgcgccc gaagtgctgc ccgccatcac
      841 gtccaggcgc ggatcggccg catcggccct ggataccagc ggggcctggg aatccttgcg
      901 cggatcgcgc tcgtgcaggc catcggtgtc cgtgaggatc accagcagat cggcttccag
      961 cagattggtg accagcgccg ccagagtatc gttgtccccg aagcggatct cgtccgtggc
     1021 gacggtatcg ttctcgttga cgatggggat gacgcccagg tcgagcaggc gggtcagggt
     1081 ggcccgggca ttgaggtagc gctcgcggtc cgccagatcg tcgtgggtca gcaggaccat
     1141 ggcggtgtgt cttgcgtgcc cggcaaaagc gcgctcgtaa gcctgcatga gacccatctg
     1201 ccccacggcc gctgcggcct gctgctcgtg cacgcccaca ggccgcgagc tccagcccag
     1261 acggctgacg ccctcagcca ccgctccgga ggacacgatg agcacctgca ctcctgcgtc
     1321 catcagccgg ttcacctgct cgcaccacga ccccatggcc gatgtggcca gcccagcgcc
     1381 gtcgtcggtc agcagcgcgc tgccgatctt gaccacgacc cgtcgggcgg cggccagccg
     1441 cgcacgcacc tcgtcactcg gaatatccct gctcattcgg gaacgtacgt tacctgaacc
     1501 tgatcctcgc cgtcatcgtc cgcttcgtcc ctctcctcgg agttcccccg cagcgcaccg
     1561 ggctttgcga gcacggcgcc gcgcagcacg tcctccccga ttcgggcctg cagctccgcc
     1621 tcccgttcgg cgtactcttc gtcaccggca agacgctcgc gaattccggc cacggcagcc
     1681 agcagcgcgt ccgtcagcct gccgattcca gcggtggtga cggccgacac ggcaaagagg
     1741 ggacgcccgg gaaaagcgtc ggcgaaacgc cgctcaaggc cttcgagatc ggcatccgta
     1801 accaggtccg tcttgctgag cacgagccag acgggccgtt cgcgcagtgc ctgactgtag
     1861 tcggccagct ccgcttcgat gagcctcgcg ttgtcgatgg ggtcggagcc gtccagcggc
     1921 gcgacctcca ccaggtgcag cagaatcctg gtacgcgcaa gatggcgcag aaactggacc
     1981 ccgaggccgg atccttccgc agcgccctga atcaatccgg gaatgtccgc catgacgaag
     2041 ctgttgtcgg taccggcgcc cacgaccccc aggctgggaa cgagcgtggt gaagggataa
     2101 tccgccacct tgggccgcgc cgcggagacc tgcgcgatca gcgtggactt gcccgcgttg
     2161 ggcagcccca gcagcccgac gtcggcgagc agcttgagct gcaggcgcag ccggcggacc
     2221 tcaccaggct cgccggtggt cgttctccgc ggggcccgat tgacactgga tttgaagtga
     2281 gcgttgccca gaccccgccg gccacccctg gcgacgagca gttcctgacc ggcctcggtg
     2341 agatcgccga gggtctccag ggtctcgtcg tcgatcaccg aggttcctac cggaatccgc
     2401 accagcctgg cgtcaccatg agccccggtc ttgttgcgcc cgctgcccgg ctgtccgtta
     2461 ccagctcggt acagcggctg gtagcggaaa tcgaccaggg tgttgagcga gtcgtcggca
     2521 accagaatga cgtcgccgcc atctccgccg tcaccgccgt cgggccctcc gcgggccacg
     2581 tacttctccc tgcggaagct caggcagccc ccgccgccct ttcctgcttc caccctgatc
     2641 gtggcttcgt cgatgaactg catggcgtgc tcaacgcaaa aaaaaccccg cgtccgcggg
     2701 gtctggctgg ttcaggcttc cgccgtcgcc ggctccacgc agacgaattt gcgcttgtgc
     2761 ggaccccggg tgagaaattg aacgtagccg tccgcggttg caaagagcgt gtgatcgcgc
     2821 ccgcagccca cgttgtcgcc cgggtgaaac ttggtgcccc gctgccgaac gatgatgttt
     2881 cccgccgcaa cgaactggcc gccatacttc ttgacgccaa gccgtttcga ctcggaatcg
     2941 cgaccgttcc gggtgctgcc gccagctttc ttgtgagcca tttcctgcct cccgccgttt
     3001 agccgtttat gcccgtgatc ttgagctcgg tgtaccactg ccggtggccc tgacgacgca
     3061 tgtagtcctt gcgccgcttg aacttgatca cccttatctt cttgccgcgg ccgctggaca
     3121 caacctcggc ggtgacttta cctccgtcga cgaacggttg gcccacggac acatcgtcac
     3181 cgtcggcgac catgaggact ttgtcgaaca ccacctcgtc gcccggttca cctgccagcc
     3241 gctccactcg tatgacctct ccttcggtga cccggtgctg cttgccaccg ctggagaaaa
     3301 ccgcaaacat acctaaactc ctctatccag gcggcagcac gatgccgcga cgaacaccca
     3361 cccgagggct ataattcgag ccttgctgaa gcgattgtcc cgggcttaag ctggcggccc
     3421 gtcgcgtaaa atgctccggg acggcgccga aaggcgcgta attctacggg caaggatatt
     3481 gaattacaac gagttagccg atgccaggtg acccggccat agcaaccaca acgacgcgaa
     3541 tcagcgccga cccgaacgac gcgctgacgc ccgtgtacga cctcgcgagg gacgatttcg
     3601 cggaggtcaa tcgcctcatt ccccaccagc tgacttccga cgtggagctc gtcgaggaga
     3661 tcggccgcta catagtggac agcggaggca aacgcctccg tccgctgatg gtgctgctgg
     3721 gcgcccgctg ctgcggctac gagggcgatg accacgtgcg ccttgccgcc atcatcgagt
     3781 tcctccacac cgcgaccctt ctccacgacg acgtggtgga ccgctccgag ctgcgccggg
     3841 gccgggcaac ggccaacgcg ctctggggca atgccccgag cgtgctcgtc ggcgattttc
     3901 tctactcccg ggcttttcag ctgatggtcg agctggaaga catggacatc atggcgatcc
     3961 tagcggacgc gaccaacacc atcgcagcgg gcgaggtgca gcagttcgcg agcatcggca
     4021 acttcgcaat cgacgaagcc cagtacatgg agatcatccg ctgcaagacg gcgctgctgt
     4081 ttcaggccgc cacccatact gccggcgtgc tgagcagcga agatcccgac gaagtcgccg
     4141 ctctcaaaaa tttcggcctc catttcgggc tcgcctacca gctggtcgac gactggctcg
     4201 attacgaggg cgactcctcc accctgggca agaacgtcgg cgacgacctg gccgagggca
     4261 agctgaccct gccgctgatt catgcgctgg caaacgcacc ggacgccgac gccaatgtcg
     4321 ttcgcgacgc cgtgagcgcc cgatccgccg agcgtctcgc cgaagtgctt caggtcgtgc
     4381 gttcctccgg cgcgttgagc tacacccgca gcgcggcaac ccggcaatgc gcgcgggccg
     4441 tggagtgcct aacgggactt cccgacaacg agtaccgcga cgcgctggag acgctggcga
     4501 acttctgcct ggatcgactc cgctagcggt gcgtcccgca ccctcgattc tgctggcctg
     4561 cctgtgtttc ttgcccttcg ccagcgcgct ggcggccgcg cccgcgctcg agcaatgcga
     4621 gctcagcggc agccatgggg tcggccgcgt ggaggccgtg tgcggccggc tgcctcgacc
     4681 cgagaacccc gaacagcccg acggagctcg gctcgacctt ttcatcgcga agataccctc
     4741 cctgagcccg gaacccgcac cggatgcgtt caccatcatc aacggcggtc ccggggcttc
     4801 ctcggtgtcg ctgttcgtcg acctcgccca ggcgttctcg ggcgttctgc gcgagcggga
     4861 catcgttctg gtcgaccaaa gaggcaccgg gcggtccaac gccctcgaat gcccggagct
     4921 ggagaaggcc gccgaggact actccatcga ggccgtccag gaagcaacac gggcatgcct
     4981 ggagtccctc gccggcgacc cccgtttcta taccacctcc caggccgtgc aggatctcga
     5041 ggcggtgcgg gcagcgctgg gatacgaaag gctcaacgtc tatggcgtgt cctacggaac
     5101 ccgggtttcc atgcactatc tgcgtcgtta tccggaccgg gtcagaacgt tgatcctgga
     5161 cggcgtggtg cctcccgggc tggctctcgg cgtcaacgtg gccgagaacg cgcagcggcg
     5221 cctggacgag ctgttcgccc agtgcgaccg ggcaccggag tgcagcgagg cgtttcctga
     5281 ccttcaggcc agtttcaccc agctgcagag caatctgaac gccgagccgg ccagcttgcg
     5341 gttccccaat ccgataaccg gcctggaaga aagcctggcg ttcggctacg accatctcgc
     5401 cctggtggtg cgcctgttga gctacgcccc ggaaaccgca tcgcttttgc ccctgatcat
     5461 cgaccaggcg gctggcggca actatctgcc ggcggcgtcc caggcactgc gaatcacgga
     5521 cgcgctgagc ggcaccatga gcttcggcat gcacaactcg gtcacctgca ccgaggatct
     5581 gcccttcatc gatttcagcc aggtgaactg ggatgctctg gaggcgacct atatcggcgc
     5641 cgatcaggtc cgggcccttc gcgcgatctg cgagatctgg ccgcgcgggg caatggatcc
     5701 ggacctgaag gatcccgttc aggccgacac gcccacgctc atcctgtccg gcgagctgga
     5761 ccccatcaca ccgcccgcat acggggacca gataaagccg ggcttcggca actcgatgca
     5821 catcattggt gcgggtcagg gtcacggcgt cgtatccagg ggatgtctgc cgggtctcgt
     5881 cacgcagatg gtggaggcag ggcacctggg ggacctggac gccggctgcg tcgagcgcct
     5941 gtccgcggca ccattcttca tcgatctgat gggtccagcc ccatgatccg cgccgagggg
     6001 ctcgacaaat cgtttggcga ggtcaccgcc attcgtcacg tctcgttcga tgcggccgac
     6061 ggcaaagtca ctgccctgct cggccccaac ggcgccggga agtccacctc gctcaggatt
     6121 ctggccaccg tgctgcaacc cgatgggggg aaagccgagg tcgacggcgt cgacgtcgtc
     6181 gccgacccca tggcggtgcg gcgtcagatc ggcgtgctgc cgcacgccgc agggctgtac
     6241 gcccgcctca cggcccggga gaacgtcgcg tacttcgggt ctctgcacgg catcgcgaag
     6301 tcacggctga gcgaacgcat cgatgagctg atcgagcttt tagaaatggg cgagttcgcc
     6361 gaacgccggg tcgccggttt ttcccagggt cagcggatca aggtcgctct tgcccgcgcg
     6421 ctcgtccacg acccgcgcaa cgtcctgctg gacgagccca ccaacgggct cgacgtcatg
     6481 gcaacgcggg cgctccgaga catcatcatc aggcttcgcg acgaggggcg ctgcgtcctg
     6541 ttctcgagcc acatcatgca ggaggtcgcc aacgtctgtg acgacgtcat cgtcatcggc
     6601 catggacgcg tgcttttcca ggggaccctg gacgggctcc gcgaccacgc cggcgaagcc
     6661 gatctggagg aggccttcat ccgcgtgatc gacgaggagg acgtgtgacc ggtttcgcca
     6721 tcgtccttgc caaggaatgc cgcgacaaca ttcgcgaccg tcgaacgatc atctcgtcgc
     6781 tgtctctcgc cgtcctggga cccgcgctgt tcgtcgggct gatggccttc gttctggact
     6841 ccgcggtggg agagtccagc gatccggtgg agatcaccgt cgtcggcgcc gaccacgcgc
     6901 ccgggctgat ggccttcctg gaaacccaga acacgcgcat cgagcgggtg gatctcgaag
     6961 acccccgggg tgcggtggcc ggcggatccc gaaagctggt tctgtcgatt cccgatgatt
     7021 attccgagcg cttcgcggcg ggccaggtga ccaccgtgtc cctcatctac gacagctcgg
     7081 acatgggcat gtcccgccgc aactttcgga tggcgcagca gttcatcctg gcgcacgcca
     7141 ggatgatcgg gcttctccgg ctccagcttc gcggggtcga cccggccatc gcgtcccccg
     7201 ttcaggttca ggcagtcgac acggcctcgc cggccgcccg cgcgctggcc atcctagcca
     7261 ccttgcccta cctcctggtg ctcgtcatct tcatgggcgg cttttacctg gccatcgacg
     7321 caacggccgg cgagcgcgag cacggctccc tggagccgtt gctgacccag cccatatcgc
     7381 gcggcgccct cgtgctgggc aaggtagccg ccacggcggt gttcagcgca gcctccctgg
     7441 cgttgttcct cgcaagcctg gccatctctc tgccctacgc gcccctccac aaggtgggca
     7501 tgtcgctgca catcgatgtc tcgaccgcgt tgaccgtttt cgtcatggcg ctgccgctga
     7561 tactgttcgc ggccgccctg ctgaacgtgg tcgcttcgtt cgcgaagagc tacaaggaag
     7621 cgcagactta cctgacgttc gtcatcctca tccccacgct tcccctgatc atcacgcgaa
     7681 tgatgaacgt ggagccttcc gcggccctga tgatgattcc gagcctgagc caggggacgt
     7741 tgatcaacga cgcggtggcg ggtgaacccg tgcaatgggt ttttctgctg atatccgtgg
     7801 tctcgacgac gctgacggcc ggggcgctga cgatgctggc cgtgtacctt tacaagcgcg
     7861 aacgcatatt gatctgatgt tgttcccgcg ccggggcgct tttacaatac gcggccctca
     7921 ccaggggcag tcggagtgta gctcagcttg gtagagcacc acgttcggga cgtgggggcc
     7981 ggaggttcaa atcctctc
//
LOCUS       DBNHLM010000330         8135 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1546708, whole genome shotgun sequence.
ACCESSION   DBNHLM010000330 DBNHLM010000000
VERSION     DBNHLM010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8135)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8135)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1546708"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="AC16QF_15285"
     CDS             complement(<1..637)
                     /locus_tag="AC16QF_15285"
                     /inference="COORDINATES: protein motif:HMM:NF025114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR6449972.1"
                     /translation="MSRSEVEGAEFGFDPEALRAKYAEERDKRLRADGNAQYLEVAGK
                     FAHFLDDPYVERTPRDALTDEVEVAIIGGGFGGLLAGARLREAGVEDLRIIEKGGDFG
                     GTWYWNRYPGAQCDIESYIYLPLLEEVGYIPREKYSFAPEIMAHSQAIGRKYDLYRNA
                     CFQTEVTELRWDEEDQRWEIRTNRGDRMKARFVCMSNGPLNRPKLPGIEGIE"
     gene            945..1613
                     /locus_tag="AC16QF_15290"
     CDS             945..1613
                     /locus_tag="AC16QF_15290"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6449973.1"
                     /translation="MFKLTDANLVFRALVIFGLVLFGFFLAEQQGLVSLALGSDKSRI
                     SYLILGLYGAVSVHWLWLARALGAERRRFVALENSICEGEAVDVRGGLLGGFLENLSL
                     KRDDDASALLEAFGDELANRHAFGHFASDVLLKLGLLGTVVGFILMLLPVGEIEEFDP
                     SLMQQLLSAMSGGMAVALYTTLAGLVTSTLLKLQYHLLDASAAQLVTRLAVLVDVRLK
                     RDAA"
     gene            1603..2220
                     /locus_tag="AC16QF_15295"
     CDS             1603..2220
                     /locus_tag="AC16QF_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449974.1"
                     /translation="MRHDVFRQRESQDAFTDLLFNALLGFAFMFVAAFALISDPSNTG
                     KIDSKAEVLITVRWADRHPDDVDTLVEGPRGQLVWYHNRDSGLMHLDRDDRGLFADRI
                     VLDGREVSNPINQETVTVRALQPGEYVVNVLHYQSNYSEPLPVTVKVEKLNPTVTLVY
                     YGTRQLTGVGDEQTAVRFTIGADGQVASTSELPKPLLTRAVGGKT"
     gene            2217..2783
                     /locus_tag="AC16QF_15300"
     CDS             2217..2783
                     /locus_tag="AC16QF_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449975.1"
                     /translation="MTGPILGLTLAYVAVAALLLCLNLATRFSPWVKGIAVVLVTALY
                     ASAWLGYQGLLGWASPAALPETFRVLWITVEEPDKESGAPGTIFFWVRELDEAGFPVA
                     PPRAHRIAWDEASARAAQEALEKLEEGERLNGRLSRGVVDEGEGRPEETTGYAAEQSV
                     TGAVGRRPDFEFVQVPPPALPPKAVPGG"
     gene            complement(2787..5009)
                     /locus_tag="AC16QF_15305"
     CDS             complement(2787..5009)
                     /locus_tag="AC16QF_15305"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003239772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HIR6449976.1"
                     /translation="MRAFIWIALVAIAAVVYFFATSTAPDGATEDGPTAAGGGTLESG
                     PSVGLIDDARINAADSEPGNWLAYGRTYEEQRFSPLTQINRESVGELGLAWYKDMGTN
                     RALEATPIVVDGIMFFTTTWSRVHAVDARTGETVWFFDPEVPGETGRKACCDVVNRGV
                     AVYQGKVFVGSLDGRLIALDAATGEKVWEVDTIVDRTRAYTITGAPRAARGKIFIGNG
                     GAEYGVRGYVTAYDAQTGEQVWRFYTVPGDPSKPFEHPEMEMAAKTWKGGEWWEIGGG
                     GTVWNSIVYDPDFNHVYLGVGNGSPWTRVIRSPGGGDNLFLSSIVALNADTGEMQWYY
                     QTTPGDNWDYTAVQDIALADMEVDGEMRKVLLQAPKNGFFYVIDRTNGELLRANPYVA
                     VNWATHVDMATGRPVENPAMDYADTAKWILPGPLGGHNWQAMSVDVEAGLVYIPALEN
                     PLIYGMPDEWQQTGVYKRDPGRWNTGIELGAIAQLFVDNIAEQPTPRGLLKAFDPLSG
                     EERWSVEIPHYWNGGVLGTAGGLVFQGNAMGMFKAYDKSTGEALWEFNTYTSMLAPPV
                     TFELDGEQYVSILTGFGGGDLFGGEPLPPLSMHASLKYNNHGRLLVFKLGAKEQLPVP
                     SARDRTIPEQLVADVDVADIARGERLYNDYCAVCHGLVARSSGAIVDLRLMSAESHDA
                     FDGVVLEGLMSARGMTAFSDVLTVEDSERIHHYVRARAHEDREVALGNSEAARLTWLN
                     "
     gene            5155..5331
                     /locus_tag="AC16QF_15310"
     CDS             5155..5331
                     /locus_tag="AC16QF_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449977.1"
                     /translation="MKSWIDFRENGSSPLTKIKREYFGELRLVWKEELGNYHVRQATA
                     LVGGGGRSREVSFD"
     gene            5324..6121
                     /locus_tag="AC16QF_15315"
     CDS             5324..6121
                     /locus_tag="AC16QF_15315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6449978.1"
                     /translation="MTEEASRFVGSIPEHYDAGLGPHIFEGYAEDLAGRVAALDPAAV
                     LELAAGTGIVTRRLRDVLPAGCDIVASDLNQPMLDFAEAKFAPDESVGFEVADATDLP
                     WQDERFDVVTCQFGVMFFPDKHRSYAEARRVLRAGGHYVFNVWDAWPFNAFAEITHDV
                     VAGFFPDDPPGFYRVPFGYHDVEVIRRSLLDAGFRAASAETVQLTSAIPSAENFARGL
                     VFGNPLFQEIEQRGGDADAIHAAVADAVARRLGSQMQIRAIVVHAQK"
     gene            complement(6151..6648)
                     /locus_tag="AC16QF_15320"
     CDS             complement(6151..6648)
                     /locus_tag="AC16QF_15320"
                     /inference="COORDINATES: protein motif:HMM:NF016213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HIR6449979.1"
                     /translation="MDSAMLIDVLLWILVVGLVIVGVAGLLLPALPGAPLVFAGLLIG
                     AWIDDFAYVSWGTLITLGIMAALTYAVDFAAGALGASRFGASPRAVWGAVIGGIVGIF
                     MGLVGIIIGPFVGAVIGELSAQRSLEEAGRAGLGATLGLVIGTAIKLTLAMAMVGIFV
                     LMRFL"
     gene            complement(6720..7634)
                     /locus_tag="AC16QF_15325"
     CDS             complement(6720..7634)
                     /locus_tag="AC16QF_15325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6449980.1"
                     /translation="MQIGIHAPHLGRELDPVTVRSFVQECDTLGVHSLWVSDHVCWPA
                     DIASKYPYTEDGSFPATPEMGWLDPIGTLLFIAGCTDRVRLGTTVLILPYRPPVATAK
                     QLATLDVVSGGRLILGVGIGWMAEEAAVLGMPWDHRGRRSDEQLEIFRRLFTETEPSF
                     EGEFYSFPKVGFEPKPVQQPVPVWVGGSTPAAFRRVARFGQAFHAAFQPLAEVGREWR
                     AVQAACEEIGRDPSDLSLSLRLYLDPDSRMEPEKSVAGSAEQMRERLFELAAAGVGHV
                     VLDPVARGGVQGRLDAVRGFMTDVAPAS"
     gene            7759..>8135
                     /locus_tag="AC16QF_15330"
     CDS             7759..>8135
                     /locus_tag="AC16QF_15330"
                     /inference="COORDINATES: protein motif:HMM:NF014816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6449981.1"
                     /translation="MLKLYLMPGSCALASHITLREAGADFELKVVDFMSGEQRTPEYR
                     KVNPKGRVPALVTDRGVLTETPAILLYVAQTHPEARLAPLDDPFELARLQAFTSYLCA
                     TVHVAHAHSRRGERWADDESAIEA"
BASE COUNT         1317 a         2668 c         2706 g         1444 t
ORIGIN      
        1 attcgatgcc ttcgatgccg ggcagcttcg gccggttgag cggaccgttg gacatgcaga
       61 cgaaacgagc cttcatccga tcaccgcggt tagtgcggat ctcccaccgc tggtcctcct
      121 cgtcccagcg aagctccgtg acttcggtct ggaagcaggc atttcggtaa aggtcgtact
      181 tgcggccgat ggcctggctg tgggccataa tctccggcgc gaagctgtac ttctccctgg
      241 ggatgtagcc gacttcctcg agcagcggga gatagatgta cgactcgatg tcgcactggg
      301 cgcccgggta acggttccag taccacgtgc ccccgaaatc cccgcccttc tcgatgatcc
      361 tcagatcctc gacgcctgct tcccgaagcc gggcgccggc aagcaggccg ccaaacccgc
      421 cgccgatgat ggcgacttcg acctcgtcgg tcagggcgtc ccttggtgtg cgctccacgt
      481 agggatcgtc caggaaatgg gcgaacttgc cggcaacttc caagtactgg gcgttgccgt
      541 cggcgcgcag gcgcttgtcc cgctcctctg cgtattttgc tcgcagggct tccgggtcga
      601 acccgaactc cgccccttcg acttctgacc tgctcatggg tcgacctcca actgcacgcg
      661 gaaaagcggc ctatttagga atgccgctat gtagattgca accttcgctg gcgggagcgt
      721 atgcggccgt cggggttgga cggggatgcg gcttcttgac acctgccggc gtccttggtc
      781 gcattgtgca gtccatcggt cggcggggag gggcaccgtg attgggggaa gctgtcagtt
      841 tggtgccgtg agctttgagc ttgcggctga tccagcgagc atttgcgagc tgcccgtccg
      901 gcatatcgac ggggctgaaa cccggcagtt cgtggattag ggccatgttc aagctcacag
      961 acgccaacct cgtctttcgc gccctcgtca tcttcggtct cgtgctgttc gggttttttc
     1021 tggccgagca gcagggcctg gtgagcctgg ctctcggctc cgataagagc cgcatctcat
     1081 acctcattct cggtctttac ggcgctgtga gcgttcactg gctgtggctg gcccgcgcgc
     1141 tgggtgcgga acggcggcgc ttcgtcgcgc tggagaacag catctgcgaa ggtgaggccg
     1201 tcgacgtcag aggcggtctg ctcggcgggt ttctcgagaa cctctccctc aagcgcgatg
     1261 acgacgcctc ggctctgctc gaagcgtttg gcgacgagct ggcgaaccgg cacgcgttcg
     1321 gtcatttcgc cagcgacgtt ctgttgaagc tcggcctgct cggcaccgtg gtcggcttca
     1381 tcctcatgct cctgcccgtc ggggagatag aagagttcga cccgagcctc atgcagcagc
     1441 tgctgagtgc catgagcggc ggcatggcgg tagcgctcta cacgacgctg gccgggttgg
     1501 tcaccagcac cctgctcaag ctgcaatatc acctgctcga tgcgtcggcc gctcagctcg
     1561 tcacgcggct tgcggtgctg gtggacgtga ggctgaagcg cgatgcggca tgacgtcttc
     1621 cggcagcgcg aatctcagga cgctttcacc gacctgcttt tcaacgcgct gctgggcttc
     1681 gccttcatgt tcgtcgccgc gttcgccctg atcagcgatc cgagcaacac ggggaagatc
     1741 gactcgaaag ccgaggtgct catcaccgtg aggtgggccg accggcaccc ggacgatgtc
     1801 gataccctgg tcgaggggcc aagagggcag ctggtctggt accacaatcg cgacagcggg
     1861 ctcatgcacc tggaccgcga cgatcggggc ctgttcgcgg atcggatcgt tctcgacggc
     1921 cgggaggttt ccaacccgat caatcaggag acggtgaccg ttcgcgcgct gcagccgggt
     1981 gagtacgtgg tgaacgtgct ccactatcag tcgaactaca gcgagccgct gcccgtcacc
     2041 gtgaaggtgg agaagctcaa ccccacggtt acgctcgtct actacggcac ccggcagctc
     2101 accggggtgg gggacgagca gacggccgtt cggttcacca tcggcgccga cggacaggtc
     2161 gcgagcacca gcgagctgcc gaagccgctc ctgacccggg cagtgggcgg caagacgtga
     2221 ccggcccgat tctcgggctc accctggcct acgtggcagt ggccgccctg ctcctgtgcc
     2281 tgaacctggc gacccgcttc tcgccgtggg tcaagggaat tgcggtggtg ttggtcactg
     2341 cgctttacgc gtcggcctgg ctcggctacc agggtctgct cggctgggca tcgcctgctg
     2401 cgctgccgga gacgttccgc gtgttgtgga tcacggtgga ggagccggac aaggaaagcg
     2461 gcgcccccgg caccatcttt ttctgggtgc gtgagctgga cgaggcgggt tttccggtcg
     2521 ccccccccag ggcgcaccgg attgcctggg acgaagcgtc tgccagggcg gcccaggagg
     2581 ctcttgaaaa gctggaggag ggggagcggc tcaatgggcg tttgagccga ggcgtggtgg
     2641 acgagggcga aggccgtccc gaagagacca ccggctacgc ggccgagcag tccgtgacgg
     2701 gcgccgttgg gcgccgcccg gacttcgagt tcgtgcaggt cccgccgccg gcgctgccgc
     2761 ccaaggcggt cccgggcggg tagcggtcag ttcagccagg tgagcctggc agcctccgag
     2821 tttccgagcg ccacttcccg gtcctcgtgt gcccgggctc tgacgtagtg gtgaatgcgt
     2881 tcggaatcct ccaccgtgag cacgtcgctg aacgccgtca tgccccttgc ggacatcagc
     2941 ccctcgagaa cgaccccgtc gaaggcgtcg tgggactccg cggacatcag gcgcaaatcg
     3001 acgatggccc cgctggagcg tgcgaccagc ccgtggcaga cggcgcagta gtcgttgtag
     3061 aggcgctcgc cccgggcgat gtccgccacg tcgacgtcgg cgaccagctg ctccggaatg
     3121 gtccggtcac gggccgaggg aaccggcagc tgctctttgg cgccaagctt gaacaccagc
     3181 aaacggccgt ggttgttgta cttcagcgaa gcgtgcatcg acagcggcgg caggggctcg
     3241 ccgccgaaca ggtcgccgcc gccgaacccg gtgaggatcg atacgtattg ctcgccgtcc
     3301 agctcgaaag tgacgggtgg tgcgagcatg gaggtgtaag tgttgaactc ccacagcgcc
     3361 tcgccggtgg atttgtcgta ggccttgaac atccccatgg cgttgccctg gaagaccagc
     3421 ccgccggcgg tgccgagcac gccgccgttc cagtagtgtg ggatctccac cgaccagcgc
     3481 tcctcgcctg agagcgggtc gaaggctttg agcagaccgc gaggcgtggg ctgctcggcg
     3541 atgttgtcca cgaacagctg cgcgatggcg ccgagctcga tccccgtgtt ccagcggccc
     3601 gggtcgcgct tgtagacgcc cgtctgctgc cactcatccg gcatgccgta gatcagcggg
     3661 ttctccagcg ccgggatgta gaccaaaccg gcctccacgt ccacggacat ggcctgccag
     3721 ttgtgcccgc cgagcggtcc cgggagtatc cacttcgctg tgtcggcgta atccatggcc
     3781 ggattttcca ctggccggcc ggttgccatg tccacgtggg tggcccagtt caccgcgacg
     3841 tagggattgg cgcgcagaag ctcgccgttg gtccggtcga tgacgtagaa gaagccgttc
     3901 ttgggcgcct ggagaagcac cttgcgcatc tctccgtcca cttccatgtc ggcaagcgcg
     3961 atgtcctgaa cggccgtgta gtcccagttg tcgcccgggg tggtctggta gtaccactgc
     4021 atctcgccgg tgtccgcgtt cagggcgacg atggaggaga ggaacaggtt gtcgccgccg
     4081 cccggtgatc ggatcacccg cgtccagggg gaaccgttgc ccacgccgag gtagacgtgg
     4141 ttgaagtccg ggtcgtagac gatggagttc cacaccgtgc cgccaccgcc gatctcccac
     4201 cactcgccgc ccttccaggt cttcgcggcc atctccatct ccggatgctc gaagggcttg
     4261 gagggatcgc cgggcaccgt gtagaagcgc cagacctgct cgccggtctg ggcatcgtag
     4321 gcggtgacgt agccgcgcac gccgtactcg gcgccgccgt tgcctatgaa gatcttgccc
     4381 cgtgccgctc gcggcgcgcc ggtgatggtg taggcccggg tacgatcgac gatggtgtcc
     4441 acttcccata ccttctcgcc ggtggccgcg tccagcgcga tgagccggcc gtccaggctg
     4501 ccgacgaaga ccttgccctg ataaacggcg accccgcggt tgaccacgtc gcagcaggct
     4561 ttgcgtccgg tctctcccgg cacctcggga tcgaagaacc agaccgtctc cccggtccgt
     4621 gcgtccaccg cgtggacgcg gctccaggtg gtggtgaaga acatgatgcc gtccacgacg
     4681 atgggcgtgg cctccagcgc acggttggtg cccatgtcct tgtaccaggc gaggcccagc
     4741 tccccgacgc tttcccggtt gatctgagtc agcggcgaga agcgctgctc ctcgtaggtg
     4801 cgcccgtagg ccagccagtt tccgggctcg ctgtcggcgg cgttgatgcg cgcgtcgtct
     4861 atcagcccga cggacggccc agattccaga gtgccgccgc cggccgcggt cggcccatcc
     4921 tccgttgccc cgtcaggagc ggtggatgtc gcgaaaaagt agaccacagc agcgatcgcg
     4981 accaatgcga tccagatgaa cgcgcgcatg atgtctccct ttacctgcgt cggggatcat
     5041 accgccgctc tcccgaccgc gacaaacttc gtgcgcggcg tatgccgcgg ggttcgcgct
     5101 gcgcttctgg cgggcgtgtg gttcgaggga tagactgcaa ttgggagggt tgccatgaag
     5161 agctggatcg actttcgcga gaacggctcc tcgccgctga ctaagatcaa gcgggagtac
     5221 ttcggggagc tgcgtctggt ctggaaagag gagctcggca actaccatgt ccggcaggcg
     5281 actgcgctgg tgggcggcgg cggacggtcc cgggaggtga gctttgactg aagaagcttc
     5341 ccggttcgtc ggctccatac ccgagcacta cgacgccggc ctgggtccgc acatcttcga
     5401 aggctacgcc gaggacctgg ccggtcgggt tgccgcgctg gatcccgccg ccgttctcga
     5461 gctcgcggca gggacgggga tcgtcacccg acggctccgc gacgtccttc cggcgggttg
     5521 cgacatcgtc gcgtccgacc tcaaccagcc catgctggat ttcgctgagg cgaagtttgc
     5581 gccggacgaa agcgtggggt tcgaggtcgc cgatgccacc gacctgccgt ggcaggatga
     5641 gcgcttcgac gtggtgacgt gccagttcgg ggtcatgttt tttcccgaca aacaccgttc
     5701 ctacgccgag gcccgccggg tgctgcgcgc gggcggtcac tacgtcttca acgtctggga
     5761 tgcctggccg ttcaacgcct tcgcggagat cacccacgac gtggtggccg gcttcttccc
     5821 tgacgatccc cccggtttct accgcgtgcc cttcggctac cacgacgtcg aggtcattcg
     5881 ccgctcactg ctcgacgccg gcttccgggc cgcgtccgcg gagacggtcc agctgacgtc
     5941 tgccataccc tcggcggaga acttcgcgcg aggcctggtg ttcggtaacc cgctgtttca
     6001 ggaaatcgag cagcgcggcg gcgatgcgga tgcgatccac gcagctgtcg cggacgccgt
     6061 tgcccggcgt ctgggcagcc aaatgcagat ccgcgccatc gtcgtgcacg cgcagaagta
     6121 gacgtctgcc tgataaggtg cccggcgccg tcagagaaag cgcatcagga cgaagatgcc
     6181 caccatggcc attgccaggg tcagcttgat ggcggtgccg atcacgagcc ccagggtggc
     6241 gccgagtccg gccctgccgg cctcctccag ggagcgctgc gcggagagct cgccgatgac
     6301 ggcgccgacg aagggcccga tgatgatgcc aaccaggccc atgaagatgc ccacgatgcc
     6361 gccgatgacg gctccccaga ccgcccgcgg cgacgcgccg aagcgtgatg cgcccagggc
     6421 gccggcggcg aaatccacgg cgtaggtgag agcggccatg atccccagag tgatgagggt
     6481 gccccagctc acgtaggcga aatcgtcgat ccaggcgccg atgagaagtc cggcgaacac
     6541 caggggtgcc cccggcaggg ccggcagcag cagcccggcc acgccgacga tcaccaggcc
     6601 cacgaccaga atccagagca gcacgtcgat gagcatcgca gagtccatgg gcctattgtg
     6661 gcgcgtcagt gctcacggag aaaccgttcc gcaggggcct gcacggccgg cttcgctcct
     6721 caggacgcgg gggcgacgtc ggtcatgaat cccctaacgg cgtccaggcg cccctgcacg
     6781 cccccgcggg caaccggatc cagcaccacg tggcccacgc cggcagcggc cagctcaaaa
     6841 agcctctcgc gcatctgctc cgcgctcccg gccaccgact tttccggctc catgcggctg
     6901 tccggatcga gatagagccg cagagacagg ctcaggtctg agggatcgcg cccaatttcc
     6961 tcgcaagccg cctgcaccgc ccgccattcc cggccgacct cggccagcgg ctgaaaggcg
     7021 gcgtgaaagg cctgaccgaa tcgcgccacc cgccggaacg cggccggcgt ggatccccct
     7081 acccagaccg gaaccggctg ttgcaccggt ttcggttcga agccgacctt ggggaagctg
     7141 taaaactctc cttcgaagct cggctccgtt tcggtgaaca gccgcctgaa tatctccagc
     7201 tgctcgtcgg accggcgtcc gcgatgatcc cagggcatgc ccaggacggc ggcctcctcg
     7261 gccatccagc cgatgccgac gccgagaatg agccggccgc ccgagacaac gtccagggtg
     7321 gccagctgtt tggctgtggc caccggcggg cgatagggca ggatcagcac cgtggtgccc
     7381 agacgtaccc ggtcggtgca tccggcaatg aagagcagcg ttccgatggg gtccagccag
     7441 cccatctcgg gcgtggcggg aaaggatccg tcttcggtgt agggatattt cgaggcgatg
     7501 tctgccggcc agcagacgtg gtcgcttacc cacagggagt ggacgccgag ggtgtcacac
     7561 tcttggacga atgaacgaac cgtcaccgga tcgagctcgc gcccgaggtg cggcgcgtga
     7621 atgccgatct gcattgtgga gcctcccagt ggcttcggtt tgagtctagc agaccgcagt
     7681 atcggggacg cgacgtgagc ccccagccga gctaaagtga agatcccatc tgcggttgtg
     7741 ggctcacagg gagatcagat gctgaagctc tacctcatgc cgggcagctg cgccctggcc
     7801 tcgcacatca cacttcgcga ggcgggcgcc gacttcgagc tcaaggtggt ggatttcatg
     7861 tccggcgagc agcgcacgcc ggagtacagg aaggtgaatc ccaagggccg cgtccccgcg
     7921 ctcgtcacgg accggggcgt gctgacggag acgcccgcca tcctgctgta tgtcgcccag
     7981 acccatcccg aagcccgatt ggccccgctg gacgaccctt tcgagcttgc caggctgcag
     8041 gcgttcacca gctacctctg cgccacggtg catgttgccc acgcccacag ccgccgcggc
     8101 gagcgctggg cggacgacga gagcgccatc gaggc
//
LOCUS       DBNHLM010000331        11169 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1486528, whole genome shotgun sequence.
ACCESSION   DBNHLM010000331 DBNHLM010000000
VERSION     DBNHLM010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11169)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11169)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11169
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1486528"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="AC16QF_15335"
     CDS             <1..396
                     /locus_tag="AC16QF_15335"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6449982.1"
                     /translation="LEAARNTEPFPATRTTLATLRRNGKRLGVVTRNCSTAVRTVFSD
                     IDVYIDAVLARDDVDFLKPDARHVRQALSVLDGTPERAAMIGDGRLDMHVGRDLGMTC
                     VGVLTGSADADALLEAGAHIIVGALEELL"
     gene            494..787
                     /locus_tag="AC16QF_15340"
     CDS             494..787
                     /locus_tag="AC16QF_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449983.1"
                     /translation="MHRATQARNAIAPLLDQLIVQLDAEGRASQRAYFARIRRSLLLA
                     RHEADLATPIRELSTSVAVGFVFSNDADVLMSRILEKVDQLAGELEFSRPVIH"
     gene            784..1662
                     /locus_tag="AC16QF_15345"
     CDS             784..1662
                     /locus_tag="AC16QF_15345"
                     /inference="COORDINATES: protein motif:HMM:NF013607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6449984.1"
                     /translation="MTSPARDAPITGGCLCGAVRFAARGRPLWTAHCHCESCRRHTSS
                     LLATYAGFPSEDVEFSGDQPALIRSSPQAERGHCGRCGSPLCYRSARWPGETHLFLPS
                     FDDPARFEVEAHVMFDERVAGFDVYDDLPRYATGSPTPIAWGPRPAARVLFLCTGNSA
                     RSIIAQTIANDLRATVNGERVRAHSAGSRPVGEVNSGAMEVLRAHGHDVRGLRSKSWD
                     EYTGDAAPALRWVITLCDSAAGETCPAFASRAGVPVERLHWGLPDPASGAASFEAVYQ
                     TLAARIDELIAGAAPA"
     gene            complement(1664..2878)
                     /locus_tag="AC16QF_15350"
     CDS             complement(1664..2878)
                     /locus_tag="AC16QF_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007943237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449985.1"
                     /translation="MDFNDTAEEAKFREEVRGWLEVNVPTAEELAGLNYIEAAKLWQK
                     RKYDAGWACIRWPEEYGGRGASAIDQVIWNQEEAKYPDLPQGIFGIGQGMAAPTLMTW
                     ADDEAKARYLPRLASGEDIWCQLFSEPAGGSDLAALRTKAERDGDDWVINGQKIWTSG
                     AHYSDYGILVVRTDPTVPKHKGLSYFYLDMKSPGIEIKPIKQISGDSNFNEVYFTDVR
                     IPDSQRLGAVGQGWQVSLTTLMNERASIGGGGTAVGFDSVFKLAQRVNIGDRPAIEDS
                     QVRAKLADWFCQEAGLRFTGYRSMTALSRGEIPGPENSIGKLVGAPKTQDMASFAIDL
                     MEMTGAIWDKEFAEEAGLFQGTYMAIPGLRIAGGTDEIMANIIAERVLGLPQEPRMDK
                     GIPFNEVPTGSG"
     gene            complement(2888..3988)
                     /locus_tag="AC16QF_15355"
     CDS             complement(2888..3988)
                     /locus_tag="AC16QF_15355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6449986.1"
                     /translation="MNFEFNEDQNLLREQARGFLADNCSPAVVRAVLEGDASYDADLW
                     RKIVEMGYTATVIPEEFGGLGLSYLELSVIAEELGRVAAPVPFSSSVYLATEAILLAG
                     SREQKETWLPRLAAGEVIGTFAFGESHGRPTAENLTARVTDGKLSGTKLPVPDGGAAD
                     VAVVVASNGLYLVDLAQDGVAVTDVPTLDPTRGHSRIEFAGVQAEPLGDPADGWSLTE
                     KVLDRAAVLFAWEQVGGSEASLEMAKEYALGRYAFGRPIASYQAIKHKLANMYVKNTL
                     AKSNCYYGAWALNTDASELTLAAATARVSATQAYYFASKENIQTHGGMGYTWEFDCQF
                     YYRRAKLLSVNIGSEAHWQDRLITAVEQSNAA"
     gene            4068..5993
                     /locus_tag="AC16QF_15360"
     CDS             4068..5993
                     /locus_tag="AC16QF_15360"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HIR6449987.1"
                     /translation="MDYTELGFIRVAAVAPELSIAEPATNAEKIVEHLTALEGRAVTI
                     ALFPELALTGYSCEDLFFSEQLHAAARSALLMLARSAGEAVCVVGTPYLLEDGRLLNC
                     AAVIADGRVRAMIPKIAQPNYGEFYERRWFVSGAGVDVSVDDPELGSFQVATNQLMRI
                     GNMRFAVEICEDLWAPEPPGIRHSLAGAELVVNLSASNELVAKADYRRDLVHMASARG
                     ICGYVYASSGPLESTKDLVFGGHLIACENGQLLGESPRYAFEPQTLITDIDIAKLRHD
                     RMQNTTFTNAPRPAPYHVVDVLQRPRPIAALERVYTAHPFVPDDEAQFEARAAEILDI
                     QTTGLARRCLAANTETLVIGLSGGLDSTLAFLVCLDTLDKIGRPRQSIQAITMPGPGT
                     TEHTLESARGLCAAAKVALKEIPIHEAVAQHLDALEHTGRDDLVFENTQARERTQILF
                     DYANKVSGIVVGTGDLSELALGWCTYNADHMASYNVNASVPKTMMAYLVRWYAKHRAG
                     EALAGVLQRVLQTPISPELIAPDTAGSISQETEAIIGPYELHDFFLFHFLRNGAGPAK
                     IFHLARLAFTGSYADDEIKRWLRLFFQRFFTQQFKRTTLPPGPKVGSVSLSPRGDWRM
                     PDEAGVQRLLADIDDLG"
     gene            6048..6974
                     /locus_tag="AC16QF_15365"
     CDS             6048..6974
                     /locus_tag="AC16QF_15365"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6449988.1"
                     /translation="MRYLSLSITGLALLAAIGTAHAADAPFCGDEGVWVQILGAGGPE
                     LDDGQAGPSYLIWRDNHARLLVDAAPGSSAAFDRAGAEFEDLDAIAFTHLHADHSADF
                     PAFIKGSYFAERDRPLTVLGPSGEGVFPDTVTFVERMIGKDGAYPYLADFLTYGSAGG
                     YKVNARNVPSTGQRRWSRFGTEHIRLSAIPVHHDIVPAVAWRVEVGDKTIVFTGDFSN
                     QKNVIPSFAKDADALVVSHAIPETARGAARELHAIPSQLGQIAAQANARMLILGHRMN
                     RTRGRETQSREAIDKHYQGSVIFANDMECWGL"
     gene            7009..7656
                     /locus_tag="AC16QF_15370"
     CDS             7009..7656
                     /locus_tag="AC16QF_15370"
                     /inference="COORDINATES: protein motif:HMM:NF017819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HIR6449989.1"
                     /translation="MKGTGDSPAATAEHEPLLTCWFGPLEAGFADAAHRERWFAPTRS
                     FDAALAERFAPLVATAAAGGLDEWQQTPRGRLAYVLVCDQLPRNIYRGTARAFATDRR
                     AVAAAREAVRIGVDLALALEERAFLYLPFQHSEDILDQHLAVGLFTALRDASPKGYRD
                     LTGGYLRSAQQHRDLIERFGRFPHRNAALGRASTTEEAAYLEDGKTFGQAADAKG"
     gene            complement(7686..8975)
                     /locus_tag="AC16QF_15375"
     CDS             complement(7686..8975)
                     /locus_tag="AC16QF_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016982861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6449990.1"
                     /translation="MTLRRLAPLLLVLNAAGSLAEVSPEIRRGAEAVEAQVIAWRRDI
                     HAHPELSNREFRTAALVAEHLDSLGMEVTSGTAHTGVVGVLRGGLPGPVVALRADMDG
                     LPVEERTGLPYASKVRSTYNGLDVGVMHACGHDNHVAILMGAATVLSGMRERLPGTVK
                     FVFQPAEEGPPAGEDGGARMMVQEGVLEDPDVEAIFGLHISQADTVGFANYRPGGAMA
                     SAQRFEITITGRQTHGARPWQGVDPIVAGAHVVTALQTIVSRQVDISKAPAVVTVGSF
                     HGGVRNNIVPDSVKLEGTIRTFDADMRADIHARIRRIATTVAESMGATADVYINDGVP
                     VTYNDPALTQAMLPTLHKVYGADRVRYALPITGAEDFSFFQEQVPGFYFFIGGRPADM
                     AAEDAIPNHSPLFFVDEGALKLGVQAMSQLAVDYLNR"
     gene            9077..9715
                     /locus_tag="AC16QF_15380"
     CDS             9077..9715
                     /locus_tag="AC16QF_15380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6449991.1"
                     /translation="MEFVIAATDDLLATTRAVRKRLDLTRPVPREIINECLELAVQAP
                     TGSNSQGWRWVVVDDAAKRKALADLYREAAGGYLSGAEEQANESGNQQTQRVFSSAIY
                     LMEHLHEVPVHVIPCLEGRPPEDAPPITYAGVYGSIYPAVWSFQLALRARGLGSTLTT
                     LHLFKEREAAELLNIPDNVMQVALLPVGYTVGTDFKRADRPPVAGITHWNEW"
     gene            complement(9800..10195)
                     /locus_tag="AC16QF_15385"
     CDS             complement(9800..10195)
                     /locus_tag="AC16QF_15385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6449992.1"
                     /translation="MLAGLRESPSGFAMTRCAALILVAILLCGCAVSSNRPTQLVSGS
                     AAYPEDARAQGIEGLVVVRYDVAVDGRVINARVVRAEPPDVFDDAALASVRSWRFNPA
                     LADGVPRATQDIESTVTFKLDGGDEYADY"
     gene            10275..10520
                     /locus_tag="AC16QF_15390"
     CDS             10275..10520
                     /locus_tag="AC16QF_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HIR6449993.1"
                     /translation="MSIPTEDDHDPDPPERGPLTLTQTVASALAAAFGVQSSRNRTRD
                     FSRGRPAQFIAVGVVLTALLILGIAALVHLVLAGIES"
     gene            complement(10517..>11169)
                     /locus_tag="AC16QF_15395"
     CDS             complement(10517..>11169)
                     /locus_tag="AC16QF_15395"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6449994.1"
                     /translation="AARAFARYQNLLADLPAPPLDDPIPGFMRLDGYLAALDAATAGA
                     AGEHGAEDLLGFVEGRRDLAQVLQDPGKTGGYIHGDCKINNLLFRENSAEVVCVVDLD
                     TTMHGHWAWDFGDLARSGAVTEAGALSLELFGALVEGFAGEKEAALGADELVLAPRYV
                     CFMLGVRFLTDHLDGDRYFKIAARGDNLRRARAQFRLLEEMEARESEMAEVAASRR"
BASE COUNT         1875 a         3876 c         3581 g         1837 t
ORIGIN      
        1 ctggaggctg cccggaacac cgagccgttc ccggccacgc ggaccaccct cgctactctg
       61 cggcggaacg ggaagcgtct tggcgtggta acgcgcaact gctcgacggc ggtgaggacc
      121 gtctttagcg acatcgacgt atacatcgac gccgtcctgg cacgcgacga cgtggacttt
      181 ctcaaacccg acgcccggca cgtgcgccag gcgctgagcg tcctcgacgg gacccctgag
      241 cgagcggcca tgatcggcga cggccgcctc gacatgcacg tcggtcggga tctgggcatg
      301 acgtgcgtgg gcgtgctcac cgggtccgcc gacgcggacg ccctgcttga agcaggcgcc
      361 cacatcatcg tgggcgcgtt ggaggagctg ctgtgacgtc gcttccggca ccgggcgcga
      421 caggctggcc ttccgcggcg actgggttca gaattgcggg cagtcgcgtc ggtcctggaa
      481 cctggagaag gagatgcatc gagccacgca agcacgcaat gccatcgccc ctctgctgga
      541 ccagctcatc gtccagctgg acgccgaggg ccgggcttcc caacgcgcct acttcgctcg
      601 aatccgccgt tccctgcttc ttgcacgaca cgaagccgac ctagccacgc ccattcgcga
      661 gctgtccacg tctgttgcgg taggcttcgt tttctcaaac gacgccgacg ttctgatgtc
      721 ccggatcctc gagaaggtcg atcagctggc cggcgagctg gagttttccc ggcccgtaat
      781 ccattgacga gcccggcccg cgacgcgccg atcaccggcg ggtgcctctg cggcgcggtc
      841 cggtttgccg ccagaggtcg tcccctctgg acggcccact gtcattgcga atcctgcaga
      901 cggcacacca gctccctgct cgccacctac gcgggctttc cgagcgagga cgtagagttc
      961 agcggggacc agcccgccct gatccgttcg tcgccccagg ccgagcgcgg gcattgcggc
     1021 cgctgcggtt ccccgctgtg ctaccgctcg gcgcgctggc cgggtgagac gcacctgttc
     1081 ctgcccagct tcgacgatcc ggctcgattc gaagtcgagg cccacgtcat gttcgacgag
     1141 cgcgttgccg ggttcgacgt gtacgacgat ctgccgcgct acgccaccgg atccccgacg
     1201 cccatcgcgt ggggtcccag gccggccgcg agggtgctgt tcctgtgcac gggaaactcg
     1261 gcacgaagca tcattgccca gaccatcgcc aacgacctgc gagcgaccgt gaacggagag
     1321 cgggtgcgtg cccacagcgc cggcagccgc cccgtcggag aggtgaattc gggtgccatg
     1381 gaggtgctcc gggcccacgg tcacgacgtg cgcggcctgc gcagcaaatc ctgggacgag
     1441 tacacgggcg atgcggcgcc cgctctgcgg tgggtcatca cgctgtgcga cagcgcggca
     1501 ggggaaacgt gccccgcgtt tgcctcgcgg gccggcgtgc ccgtcgagcg gctgcactgg
     1561 ggcctgccgg atccggcatc cggggcggct tccttcgagg ccgtgtacca gacgctggcc
     1621 gcccgcatcg atgagctgat cgcgggcgcg gcgcccgcct agatcagcct gatcccgtgg
     1681 ggacctcgtt gaacgggatc cccttgtcca ttctcggttc ctgaggcagc cccagcaccc
     1741 gctcggcgat gatgttcgcc atgatctcgt cggtgccgcc ggcgatcctc aagcccggaa
     1801 tggccatgta ggttccctgg aacaacccgg cctcctcggc gaactccttg tcccagatcg
     1861 cgcccgtcat ttccatcagg tcgatggcga aagacgccat gtcctgtgtc ttgggtgcgc
     1921 cgaccagctt gccgatggag ttttccggtc ccgggatctc gccccgggaa agcgccgtca
     1981 tggagcggta gccggtgaag cgcagaccgg cttcctgaca gaaccagtcg gcaagcttcg
     2041 cccgcacctg ggagtcctcg atggccggcc tgtcgccgat gttgacccgc tgggcgagct
     2101 tgaaaacgga gtcgaagccg acggcggtcc cgccaccgcc gatggaagcc cgttcgttca
     2161 tcagcgtggt caacgaaacc tgccagccct gacccacggc gcccaggcgc tggctgtcgg
     2221 gaatcctcac gtccgtgaag tagacctcgt tgaagttcga gtcgccggaa atctgcttga
     2281 tcggcttgat ctcgatgccc ggggatttca tgtccaggta gaaatagctc agccccttgt
     2341 gcttcggcac cgtggggtcg gtcctgacca cgaggatccc gtagtctgag tagtgcgcgc
     2401 ccgaggtcca gatcttctgg ccgttgatca cccagtcgtc gccgtcacgc tccgccttgg
     2461 tcctgagcgc cgcaaggtcc gagcccccgg cgggctcgct gaacagctgg caccagatgt
     2521 cctcgccgct ggcaaggcgc ggaagatagc gggccttggc ctcgtcgtcc gcccaggtca
     2581 tcagcgtggg ggcggccatg ccctggccga tgccgaatat gccttgcggc agatcgggat
     2641 acttggcttc ctcctggttc cagatgacct gatcgatggc tgaagcgccg cgcccgccgt
     2701 actcctccgg ccagcgaatg caggcccagc cggcgtcgta cttgcgcttc tgccacagct
     2761 tggccgcctc gatgtaattg agccctgcga gctcctcggc ggtgggcacg ttgacttcga
     2821 gccagccgcg cacttcctct ctgaattttg cttcttccgc ggtgtcgttg aaatccatga
     2881 ctgctcctta tgccgcgttg ctctgctcga cggcggtgat caaacggtcc tgccagtggg
     2941 cctcgctgcc gatgttcacg gacagcagct tcgcccggcg atagtagaac tgacagtcga
     3001 attcccaggt gtatcccatg ccgccatggg tctggatgtt ttccttggag gcgaagtagt
     3061 aggcctgagt ggcggacacc cgcgccgtcg ccgcggccag ggtcagctcc gatgcgtccg
     3121 tgttgagcgc ccacgccccg tagtagcagt tagacttggc cagggtgttc ttcacgtaca
     3181 tgttggccag cttgtgcttg atggcctgat aagaggcgat ggggcggccg aaagcgtagc
     3241 gcccgagagc gtattccttg gccatctcca gcgacgcttc cgagccgccc acctgctccc
     3301 aggcgaacag cacggcagcc cgatcgagca ccttctcggt gagcgaccaa ccgtcggcgg
     3361 ggtcgcccag gggctccgct tgaacgcccg cgaactcgat gcggctgtgg ccgcgggtcg
     3421 ggtccagggt cggaacatcc gtcaccgcaa ccccgtcctg ggcaagatcc accaggtaca
     3481 gcccgttgct cgccacgacc acggcaacgt ctgccgcccc gccgtcgggc accggcagct
     3541 tggttccgga gagcttgccg tctgtcacgc gggcggtcag gttctccgcg gtggggcgcc
     3601 catggctctc gccgaaggcg aacgttccga tcacctcgcc ggcggccagt cgcggcagcc
     3661 aggtttcctt ctgctcccgg gagccagcca gcaggatggc ttcggttgcc aggtaaacgg
     3721 atgacgagaa cggcaccgga gcggcaacgc gaccgagctc ctcggcgatc acggacagct
     3781 ccaggtagga gagcccgagc cccccgaact cctcgggaat caccgtggcc gtgtaaccca
     3841 tctccacgat cttccgccag aggtcggcat cgtaggaggc atcaccctcc agcacggctc
     3901 tgaccaccgc cggagagcaa ttgtcggcga gaaagccccg cgcctgctcc cgcaagaggt
     3961 tctggtcttc gttgaattcg aagttcatga gcatccccag aatcgccaag aacgggcgat
     4021 tttccatgct ttgggtttaa aacgcgagcc cagttaaact gtgcaccatg gactacaccg
     4081 aactgggctt catccgcgtt gccgccgtgg cgccggaact ctccatcgcc gaaccggcca
     4141 ccaacgcgga gaaaatcgtc gagcacctga cagcgctcga gggacgcgct gtaaccattg
     4201 cgctgttccc cgagcttgcg ctgaccggct actcgtgcga ggacctcttt ttcagcgaac
     4261 agctccatgc cgccgcccgt tcggccctcc tcatgctggc ccgatccgcc ggcgaggccg
     4321 tctgcgtcgt cggcacgccg tacctgctgg aggacggcag gctgctgaac tgcgcggccg
     4381 tcatcgccga cggcagggtc cgcgccatga tccccaagat cgcccagccg aattacggcg
     4441 agttctacga gcgccgctgg ttcgtctccg gcgcaggggt ggacgtcagc gtcgacgacc
     4501 ctgagctcgg gtccttccag gtggctacca accagctgat gcgcatcgga aacatgcggt
     4561 tcgcggtgga gatttgtgag gacctctggg cgccggagcc gcccggcatc cgtcacagcc
     4621 tggccggagc cgagctggtc gtgaacctgt ccgccagcaa cgagctcgtg gccaaagccg
     4681 actatcgaag ggatctggtg cacatggcca gcgccagggg aatctgcggc tacgtctacg
     4741 cgtccagcgg cccgctggag tcgaccaagg acctggtgtt cggcgggcac ctcatcgcct
     4801 gcgagaacgg ccagctcctg ggcgagagcc cgcgttacgc gttcgagccc caaacgctga
     4861 tcacggacat cgacatcgcc aagctgcgtc acgaccggat gcagaacacg acgttcacca
     4921 acgcgccgcg cccggcgccc taccacgttg tggacgtcct gcagcgcccc cggcccatcg
     4981 cggctctgga gcgcgtctat acggctcacc cgttcgttcc cgacgacgag gcccagttcg
     5041 aggcccgggc ggccgagatc ctcgacatcc agacgacggg gctcgcccgc cgatgcctcg
     5101 ctgcgaatac cgaaactctc gtcatcggcc tgtcgggcgg gctcgactcc accctggctt
     5161 tcctcgtctg cctggacacc ctggacaaga tcggccgtcc ccgccagtcc atccaggcca
     5221 tcaccatgcc gggccctggg accaccgagc acactctgga gtcggcgagg gggctctgcg
     5281 ccgccgcgaa ggtggcgctc aaggagatcc ccattcacga ggcggtggcg cagcacctcg
     5341 acgcgctgga gcacaccgga cgtgacgatc tggtgttcga aaacacccag gctcgggaac
     5401 gcacccagat tctcttcgac tacgccaaca aggtttcggg catcgtcgta ggaaccggcg
     5461 acctgtcaga gctggcgctc ggctggtgca cgtacaacgc ggaccacatg gcgagctaca
     5521 acgtcaacgc cagcgtgccg aaaaccatga tggcctacct agtgcggtgg tacgccaaac
     5581 accgcgcggg ggaagcgctg gccggcgtcc tgcagcgcgt cctgcagacg cccatctcgc
     5641 cggagctgat cgcgcccgac accgcgggca gcatcagcca ggaaaccgag gccatcatcg
     5701 gcccctacga gctgcatgat ttcttcctct tccacttcct gagaaacggg gccggtccgg
     5761 caaagatctt tcatctggcc aggctggcat ttaccgggag ttacgcggac gacgagatca
     5821 aacgctggct gaggctattc ttccagcgct tcttcaccca gcagttcaag cgcaccaccc
     5881 ttcccccggg tccaaaggtg ggctccgtga gcctgtcgcc ccgcggcgac tggcggatgc
     5941 ccgacgaggc cggcgtccag cgcctgcttg ccgacatcga cgacctgggc tgaacgccag
     6001 gggccccttg aacgaaacga cccgataacc gaaccgagga cttcagcatg cgttacctca
     6061 gcttgagcat caccggcctg gcactgctgg ccgctatcgg caccgctcat gccgccgatg
     6121 ccccgttctg cggcgacgag ggagtctggg tgcagatcct cggcgcaggc gggccggagc
     6181 tcgacgacgg ccaggccgga cccagctacc tgatatggcg cgacaaccac gcccgcctgc
     6241 tggtggatgc ggcgcccggt tcttccgccg ccttcgaccg cgccggggcc gaattcgagg
     6301 atctggacgc catcgcgttc acgcacctgc acgccgacca ctcggcggat ttccccgcct
     6361 tcatcaaagg atcctacttt gccgagcgcg accggccact gacggtgctc ggcccgtccg
     6421 gcgagggcgt cttccccgac accgtgacgt tcgtggaacg gatgatcggc aaggatggcg
     6481 cgtaccccta cctcgccgat tttctgacct acggctccgc gggaggctac aaggtgaacg
     6541 cgcgaaacgt gccttccacc gggcagcgtc gctggtcgcg gttcggcacc gaacacatca
     6601 ggctgtctgc catccccgtt catcacgaca tcgttcccgc cgtggcctgg cgggtggagg
     6661 tgggcgacaa gaccatcgtc ttcaccgggg atttcagcaa ccagaagaac gtcatcccct
     6721 catttgccaa ggacgcagac gcgctggtgg tcagccacgc gatcccggag acggcgcgag
     6781 gcgccgcgcg ggagctgcac gccatcccca gccagctcgg ccagatcgcc gcccaggcca
     6841 acgcacgcat gctgattctc gggcaccgca tgaaccggac ccgcggtcgt gagacccaga
     6901 gccgcgaggc catcgacaag cactaccagg gctcggtgat attcgccaac gacatggagt
     6961 gctgggggct ttgagccact cgcgtcaggg gcggggcgaa gcgctcgggt gaaagggaca
     7021 ggcgactcgc cggcggccac agccgagcac gaaccgcttc ttacctgctg gttcggaccg
     7081 ctcgaggcgg gcttcgcgga cgcggcccat cgggagcgct ggtttgcccc gacccgctcg
     7141 ttcgacgccg ccctcgccga acgcttcgcg ccgctggtgg ccaccgccgc cgcgggcggg
     7201 ctcgatgagt ggcagcagac gccgaggggc cgcctggcct acgtgctggt ctgcgatcag
     7261 ctgccgcgca acatctacag gggcacggcc cgggccttcg ccaccgaccg ccgggccgtg
     7321 gccgccgccc gggaggcagt tcgcatcggc gtggatctcg ccctcgccct ggaggagcgg
     7381 gccttcctgt acctgccctt ccagcactcc gaggacatcc tggaccagca cctggccgtg
     7441 ggccttttca ccgccctgag ggacgcaagc cccaagggct accgcgatct gacgggcgga
     7501 tatctgcggt ccgctcagca gcaccgcgac ctcatcgaga gattcggccg ctttccgcac
     7561 cgcaatgccg ccctcggacg cgcttccacc accgaagagg cggcctacct cgaggacggc
     7621 aagacgttcg gacaagccgc tgacgccaag ggctgacgct cttgcagcct gtcctttgcc
     7681 gtaaatcagc gattcagata atccaccgcg agctggctca tggcctggac gccgagcttc
     7741 agcgcgcctt cgtccacgaa gaacagcggc gagtgattgg gaatcgcgtc ctccgccgcc
     7801 atgtccgcgg ggcgccctcc gatgaagaaa tagaacccgg gcacctgctc ctggaagaac
     7861 gagaagtcct cggccccggt gatgggcaat gcgtagcgaa cccgatcggc accgtagacc
     7921 ttgtgcaggg tgggcagcat ggcttgggtt agcgctggat cgttgtaggt caccgggacg
     7981 ccgtcgttga tgtagacgtc cgccgtcgcg cccatggatt ccgccacggt ggtcgcgatg
     8041 cggcgaatcc ttgcgtgaat gtccgcccgc atgtcggcat cgaaggtacg gatggtgccc
     8101 tcgagcttga cggagtccgg gacgatgttg ttgcgcacgc caccgtggaa cgagccgacg
     8161 gtgaccaccg cgggcgcctt gctgatgtcc acctgacggc tgacgatggt ctgcagcgcc
     8221 gtgacgacgt gagcaccggc gacgatgggg tcgacgccct gccagggccg ggccccgtgg
     8281 gtctggcggc cggtgatggt gatctcgaag cgctgggcgc tggccatggc gccgccgggc
     8341 cgatagttgg caaaacccac ggtatccgcc tggctgatgt gcagaccgaa gatggcctcc
     8401 acgtcgggat cctcgagaac gccctcttgc accatcatcc tcgccccacc gtcctcaccg
     8461 gcaggcgggc cctcctcggc cggctgaaaa acgaacttga ccgtgccggg cagccgctcg
     8521 cgcatgccgc tgagaaccgt agccgcgccc atgaggatgg ccacgtgatt gtcatgccca
     8581 caagcgtgca tcaccccgac gtccaggccg ttgtaggtcg agcggacctt ggaggcatag
     8641 ggcaatcccg tgcgctcctc tacgggaaga ccgtccatgt cggcgcgcag ggccaccacg
     8701 ggacctggca gtccgcctcg cagcacgccc accacccccg tatgggcagt gccggaggtc
     8761 acctccatgc cgagactgtc caggtgctcg gcaacgagag ccgccgtgcg gaactcgcgg
     8821 ttggaaagct ctggatgggc atgtatgtcc cggcgccagg caatgacctg ggcctccacg
     8881 gcttccgctc cccggcgaat ctccggcgag acctcggcga gggagccggc agcgttgagc
     8941 acgagaagta gaggagcgag tctgcgcagc gtcatctggg attcctcagc cgtccccggt
     9001 ttgggcgagc ttaatgcgcc tcaagcatgc gcacaaagtg agttaaactt cccgggcatt
     9061 cagaggggga acaagcatgg aattcgttat cgccgccaca gacgacctgc tggccacgac
     9121 ccgggccgtg cgcaagcggc tcgacctgac gcggcccgtg ccgcgcgaga tcatcaacga
     9181 gtgcctggag ctggccgtcc aggcgccgac ggggtcgaac tcccagggct ggcgctgggt
     9241 cgtggtcgac gacgcggcga agcgcaaggc attggcggac ctgtaccggg aggccgcggg
     9301 cggctacctg tcgggcgcgg aggagcaggc aaacgaatcc ggcaaccagc agacccagcg
     9361 ggtgttcagc tcggccatct acctcatgga acacctccac gaggtgcccg tccacgtcat
     9421 accgtgcctg gaaggacgtc ctccggaaga cgctccgccc attacctacg cgggcgtcta
     9481 cgggtccatc tacccggccg tgtggagctt tcagctggct ctgcgtgcgc gggggctcgg
     9541 ctccacgctg acgacgctgc atctcttcaa ggagcgggag gccgccgagc tgctgaacat
     9601 tccggacaac gtgatgcagg ttgcgctgct tccggtgggg tacaccgtcg gcaccgactt
     9661 caagcgcgcc gatcggccgc cggtggcggg catcacccac tggaacgagt ggtagcgacc
     9721 gggcggcgcc cagcccgagc accacgagcg cggttaccct gaaaggatct tcggggtcag
     9781 gcggtggtcc cgaacgccat cagtaatcgg cgtactcgtc cccgccgtcg agcttgaagg
     9841 tcaccgtgct ctcgatgtcc tgggtcgccc ggggcacgcc atcggcaaga gcggggttga
     9901 aacgccagct gcgcacggaa gccaacgccg catcatcgaa aacgtccggc ggctcggcgc
     9961 gaaccacccg ggcattgatc actcggccgt ccaccgccac gtcgtagcgg accaccacga
    10021 gcccctcgat tccctgggcc cgggcatctt ccgggtaggc cgcgctgccc gagacgagct
    10081 gggtcggccg gttcgaggat accgcgcatc cgcagaggag gatcgcgaca agaatgaggg
    10141 ccgcacatcg agtcatcgca aagccgcttg gtgattcccg caagccggcg agcatggcac
    10201 cttcgctccg gccctggcaa gaccgcgccg gtcgacgacg ctgccgcccg ctcgcccggc
    10261 gctatactcc ccccatgagc atccccacag aggacgacca cgatcccgac ccgccggagc
    10321 gcggccccct gaccctgacg cagaccgtgg ccagcgctct ggccgccgcc ttcggcgtgc
    10381 agtcaagccg caaccggacg cgcgacttca gcagaggccg gcccgcgcag ttcatcgccg
    10441 tcggcgtggt gctcactgcc ctgctgatcc tcggcatcgc ggcgctcgtt catctcgtcc
    10501 tcgccggcat cgagagctag cgacgactcg cggcgacctc ggccatctcc gactcccgcg
    10561 cctccatctc ctcgagcagg cgaaactgcg cccgggcgcg gcggaggttg tcgccgcggg
    10621 cggcgatctt gaagtagcgg tcgccgtcca gatgatccgt gaggaagcga acgcccagca
    10681 tgaagcagac gtaacgcgga gcaaggacaa gctcgtccgc gccgagcgcg gcttccttct
    10741 caccggcaaa cccctcgacc agcgctccga agagctcgag agacagcgcc ccggcctccg
    10801 tcaccgcgcc tgagcgcgcc agatcgccga agtcccaggc ccagtggccg tgcatggtgg
    10861 tgtccagatc gacgacgcag accacctccg cgctgttctc acgaaagagc aggttgttga
    10921 tcttgcagtc gccatgaatg taaccacccg tcttgcccgg atcctgcagc acctgggcca
    10981 gatctcgtcg cccctccacg aatccgagaa ggtcctccgc accgtgctcg cctgccgcgc
    11041 ctgctgtggc ggcatcgagc gctgcgaggt agccgtcgag ccgcatgaag ccgggtatcg
    11101 gatcatcgag cggcggggcc ggaagatcgg cgagaagatt ctggtaacgg gcgaacgcac
    11161 gcgccgctg
//
LOCUS       DBNHLM010000332         8064 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1486648, whole genome shotgun sequence.
ACCESSION   DBNHLM010000332 DBNHLM010000000
VERSION     DBNHLM010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8064)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8064)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8064
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1486648"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="AC16QF_15400"
     CDS             <1..498
                     /locus_tag="AC16QF_15400"
                     /inference="COORDINATES: protein motif:HMM:NF025627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HIR6449995.1"
                     /translation="DSFPQFQEGDLLLSLRNRNLVMVLDAQTREIKWWMIGNWVRQHD
                     PDWIDGRIRVFDNNRDGTNEGAILGSSKIVEVDPTTNETRVVYGEKPSQFFYTERRGK
                     HQNLPDGRVLITEAEAGRVFEVDAKGGIVWEYVNRFDETYSARISQAIAYPESYFTVA
                     DWSCS"
     gene            complement(522..1559)
                     /locus_tag="AC16QF_15405"
     CDS             complement(522..1559)
                     /locus_tag="AC16QF_15405"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HIR6449996.1"
                     /translation="MDQLAHRVAFSSRRVQFLAAEIEDSLYCSNFQNFEIGPPIFVTS
                     LPRAGTTVLLTALNSVPELATHLYRDMPFIMAPVLWSRLIGGYRQEAATRERAHRDGI
                     EIGYDSPEAFEEVIWQAFWPEHFKPDGISLWSPEDADAEAKAFMHRHFQKIVYLRLGE
                     NTAAGRYISKNNANIARLDLIPHLFPGAQIIVPVREPVAHAASLKRQHENFRNLHAED
                     QFTRRYMADIGHFEFGALHRPIQFELFESLSTGLTPGDLDYWLAYWVAAFEHIGQHRE
                     SAHLVNYERVCEGGEMAGGLLCELLGLDPSNAGAIGAHFRAPPGRQHLNSHRSELRDR
                     AEALYQNLVGS"
     gene            complement(1574..1963)
                     /locus_tag="AC16QF_15410"
     CDS             complement(1574..1963)
                     /locus_tag="AC16QF_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449997.1"
                     /translation="MLPLIILTALSVAAFLWAFWLIQIVPQAKRAIDTAKLALVVMRD
                     PELEEIDRERAVQRAATRLVLFSGSLFLRSALCLVVAFIPVYVADLAGLAPLDEVLAF
                     MALWETMLIASLIVTASYIAYVNLWRR"
     gene            complement(2000..2326)
                     /locus_tag="AC16QF_15415"
     CDS             complement(2000..2326)
                     /locus_tag="AC16QF_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6449998.1"
                     /translation="MNNVTRALTGLALVALYPQMAFAYIGPGSGLSAIGTVIAVVSAF
                     FFLIVGFVWYPFKRLIRKKKVGVEGGGEMSTEDGPKGQLQSAETAVAEMDNGRESQQS
                     EYQRLT"
     gene            complement(2785..4140)
                     /locus_tag="AC16QF_15420"
     CDS             complement(2785..4140)
                     /locus_tag="AC16QF_15420"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR6449999.1"
                     /translation="MPDRPAGMPATLPGWLGYIEGQHPQQIELGLDRVRAVATRLEVL
                     KPAPVSIIVAGTNGKGSTATCAEAVLLGAGLSVGATLSPHLHVFNERIRVNGRPCEDA
                     ALVAAFEKVEAARNDVPLTYFEFAALAALEVFRSAAVDVAVLEVGLGGRLDAFNIVDA
                     QVAVVTSIGLDHQSFLGDDLETIGREKAGVFRPGQKVVLGADVTDSVRDRARALGCDI
                     AEPGHGYRIRCQSGGGWSFAGLGLDLPLVPGGALAPENCAMGLCAAVLAIGFCNAADD
                     AAPVVDGDLVLQTLARASLPGRMEMVPWRERHVLLDVAHNPAGARFLADQLRRKGCEQ
                     LTGIIGSLRDKDTEGVARWLDGAVRRWICVGLDDPRGLPAEAVAERISGVSPCETAAD
                     AEAALERARSLTGPGDVILVCGSFSIVGQVRAFLNEKKAADGRSTAKPGKTTSAKDQS
                     C"
     gene            complement(4133..4936)
                     /gene="trpA"
                     /locus_tag="AC16QF_15425"
     CDS             complement(4133..4936)
                     /gene="trpA"
                     /locus_tag="AC16QF_15425"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR6450000.1"
                     /translation="MSRLSRRLSALGGQGRKALVSFITAGDPDPSVTVPALHALVAGG
                     ADVLELGVPFSDPEAEGPVIQASSERALAKGTTLRAVLEMVARFRKDDDLTPIVLMGY
                     LNSVLAMGEETFAEAAAEAGVDGLIMVNLPPEEAGALRGLLKEKDIDLIFLVAPTTTP
                     ERAALITEQAGGFVYYVSLKGITGADNLETGSVSKQVGALKQMTELPVLVGFGIKDGG
                     TARAVAEKADGVVVGSALVRTMAGSADALAAIPALLTAQLAEIRRALDA"
     gene            complement(4933..6147)
                     /gene="trpB"
                     /locus_tag="AC16QF_15430"
     CDS             complement(4933..6147)
                     /gene="trpB"
                     /locus_tag="AC16QF_15430"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR6450001.1"
                     /translation="MSEGKGFNKYDQPVGGHFGQHGGRFVAETLMHALDELEALYERA
                     RKDPEFQAELAHDLEHFVGRPTPLYHAARLSRDLGGAQIYLKREDLNHTGAHKINNTV
                     GQALLAKRMGKPRVIAETGAGQHGVATATVAARLGLECCVYMGAEDVKRQSLNVYRMK
                     LLGAEVVPVNSGSRTLKDALNEAMRDWVTNVDTTHYIIGTVAGPHPYPMLVRDFQAVI
                     GQEARAQSLSMLGRLPDVLVACVGGGSNAIGLFYPFVDDVDVRMVGVEAGGLGLETGR
                     HAAPLSAGRVGVLHGNRTYLMEDDDGQIIETHSISAGLDYPGVGPEHSFLKDVGRAEY
                     DTVTDDEAMAGFRLLNRTEGIMPALETSHAIAWAARHARDFDRDAVMLINLSGRGDKD
                     ILTVAEIDGIEV"
     gene            complement(6125..6760)
                     /locus_tag="AC16QF_15435"
     CDS             complement(6125..6760)
                     /locus_tag="AC16QF_15435"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003104200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR6450002.1"
                     /translation="MPTKVKICGIARPEDARCVADAGADALGLIFVRQSPRFVSTGLA
                     REIAGEVAGSLSLIGLFVDADAAEVEAVLRDVDLDVLQFHGDEPGSYCEAFGVNYMKA
                     TRVSGPVDVAGLEAEYPGACCLLLDAYVPGVAGGTGVRLDLACWPEAGAKPLVLAGGL
                     TPENVADAISRVRPFGVDVSSGVEGGIKGRKDPERVRKFVSEVRRVGREGV"
     gene            complement(6813..7610)
                     /gene="truA"
                     /locus_tag="AC16QF_15440"
     CDS             complement(6813..7610)
                     /gene="truA"
                     /locus_tag="AC16QF_15440"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR6450003.1"
                     /translation="MHLALGVEYDGSAFHGFQLQKAAATVQGALEQALSRIADHPLRI
                     AAAGRTDAGVHATGQVVGFASNAERPIQAWVKGVNALTPPSVKVRWAREVDPRFHPRY
                     SATARRYMYLWYEDDVTSPTLDRWAVRVRHLDDDAMHAASRSLVGEHDFSAFRASGCQ
                     SESAHRCVHRITVHRHGPLVVLDIAANAFLLHMVRNIAAALRDVGEGRRPAGWLAETL
                     AGRDRKHVGPTAPAHGLYLVGVRYPDQAFPPGEPPPLLRSLGGLARV"
     gene            complement(7610..>8064)
                     /locus_tag="AC16QF_15445"
     CDS             complement(7610..>8064)
                     /locus_tag="AC16QF_15445"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FimV C-terminal domain-containing protein"
                     /protein_id="HIR6450004.1"
                     /translation="QEAPVKAEAAEDEGESNVMDFDLGLDDEPASTTAVEADGGESEF
                     DLELDEDEGASRGAGDQLGGDLGIDFKPEATALDADEEGSEVATIDEDEFDFDVESDA
                     DSAETKLDLAKAYIDMGDEDGARDILKEVLEEGTSDQQTKAQTLLEQL"
BASE COUNT         1567 a         2631 c         2426 g         1440 t
ORIGIN      
        1 gatagttttc cgcagttcca agaaggcgat ctcctgctat cgctacgcaa ccgcaatctg
       61 gtgatggtgc ttgacgcaca aactcgtgag atcaaatggt ggatgatcgg caattgggta
      121 aggcaacacg atccggattg gattgacggg cgaattcgtg tgttcgacaa caatcgcgac
      181 ggtacgaacg aaggcgccat tctcggtagc tcaaaaattg tcgaggttga tccgacgacc
      241 aacgagaccc gggtcgtcta cggggagaag cccagccagt tcttttacac cgagcgcaga
      301 ggcaaacatc agaacctgcc cgacgggcga gtgttgatca ccgaagcaga agccgggcgg
      361 gtattcgagg tcgacgcgaa ggggggcatc gtttgggagt acgtgaaccg gttcgacgag
      421 acgtattcgg cgagaatctc ccaggcgatt gcgtaccctg agagctactt tacggtcgcc
      481 gactggtctt gctcctgagc cttcatgcgc gcgtcgccgg actacgagcc aactaggttt
      541 tgatagagcg cctcggcccg atcacgcaat tcgcttcgat gactgttgag gtgctgtcgg
      601 ccgggcgggg cacggaaatg cgcaccgatt gcgccagcgt tgctaggatc tagaccgagc
      661 aactcgcaca acagtccgcc ggccatctcc ccgccttcac aaacccgttc gtagttgacg
      721 agatgggcgc tttctcgatg ttggccaatg tgctcgaaag cggctaccca gtaggcgagc
      781 caatagtcca agtcgcctgg ggtgagcccg gttgacagag actcaaacag ttcaaattga
      841 ataggccgat gtagcgcgcc gaactcgaag tgcccaatgt cagccatgta gcgacgcgtg
      901 aattggtcct ccgcatgaag attgcggaaa ttttcgtgct ggcgtttgag cgaggctgca
      961 tgtgccaccg gctcgcggac cggcacgatg atttgcgcgc cgggaaaaag gtgtggaatc
     1021 aaatccaagc gggcaatatt ggcgttgttt ttcgagatgt agcgtccggc agcggtgttt
     1081 tcccctaggc gtaggtaaac gattttctgg aaatgtcgat gcataaacgc tttggcctca
     1141 gcgtctgcat cctcaggcga ccacaatgat atgccatcag gcttgaaatg ctccggccaa
     1201 aacgcttgcc agatgacttc ttcgaacgcc tccgggctgt cgtacccgat ctcgatcccg
     1261 tcgcggtgcg cccgctctcg cgtagctgcc tcttggcggt aaccgccgat caggcgggac
     1321 cacaacactg gtgccatgat gaacggcatg tcgcgataaa ggtgcgtggc tagctccggt
     1381 acggagttca acgccgtgag caaaacggtg gtgccggcgc gcggcaggga agtcacgaaa
     1441 attggaggac cgatttcgaa gttctggaaa ttactgcaat agagggagtc ctcgatctcc
     1501 gccgccaaaa attgaaccct acgggatgag aaggcgacgc gatgtgccaa ctgatccaag
     1561 atagagtacg catttaccga cgccataggt tgacgtacgc aatgtagctg gctgtcacga
     1621 tcagcgacgc aatcagcatc gtttcccaca gtgccatgaa ggctagcacc tcatcgagtg
     1681 gtgcaagtcc tgccaagtcc gcaacgtaga cgggtatgaa agcgaccacg aggcataggg
     1741 cggaccggag aaacagtgat cctgagaaca acaccagtcg ggtagcggct ctttggactg
     1801 cgcgctctcg atcaatctcc tccaattcgg gatcacgcat cacgaccagc gccaacttcg
     1861 ccgtatcaat ggccctcttc gcctgaggaa cgatctgaat cagccaaaat gcccagagga
     1921 aagcagcaac tgagagcgcc gtaaggatga tcaatggcag cataacggcc tttcaggccc
     1981 tcgccttgcc tcgcagtgcc taagtcagtc gctgatattc ggactgctgg ctctccctgc
     2041 cattatccat ttctgcgacc gcagtttccg ccgattgtaa ctgccctttg gggccatctt
     2101 ccgtgctcat ttccccgcca ccttccacac cgaccttttt tttccggatc aggcgtttga
     2161 agggatacca gacgaagccg acgatcagga aaaaaaaggc cgacacgacg gcaatcaccg
     2221 tcccaatagc gctgaggccc gagcctggcc caatgtacgc aaatgccatc tgggggtaca
     2281 acgccacgag tgccaggcca gttagtgcgc gcgtcacgtt gttcatgcta ctgactctca
     2341 cctggtttcc gaggcggggg ctacctgcga catcgactgc ccgccgcgcg accccattca
     2401 tcgaaacgcg aagctgggtc ttccgagtgc tttcttgcta atgacaattg aatgccgaag
     2461 accactttaa tgggagccgc tcgacagcac cacccaaaat cctagattgg cgaaccgctt
     2521 aacagtcaca tcgtacccaa gcagcaaatc ccaccgaaat catctgacga gcccctcggc
     2581 ccaagcgaca gagaacccgc gcgttgaccg ggtgcccggc gttgagcgca ccgcatggag
     2641 agcggagaac aaggcgctcc aatctttggc ctaatcatcg aaatccgcgg aggccgatgg
     2701 caatggattc gcgctatccg tcactttcct cacgggaaat gaccaccaaa aggggaggag
     2761 cgagccgtgt accctgaggt gtagctagca ggactgatcc tttgcgctcg ttgttttacc
     2821 tggcttcgca gtagatcgtc catcagccgc cttcttctcg ttcaggaacg ctcgcacctg
     2881 acccacaatc gagaacgacc cgcacacgag gataacatcg ccgggcccgg tcaacgagcg
     2941 cgcgcgctcc agcgcagctt ccgcatccgc agcggtctcg cagggcgaca cgccggagat
     3001 acgctcggct accgcctccg cgggaaggcc acgcgggtca tccaagccga cacagatcca
     3061 ccgtcgcacg gccccgtcca gccatcgggc gacaccctcg gtgtccttgt ccctgagcga
     3121 tccgatgatt ccggtcagct gttcgcaacc cttcctgcgc agctgatcag caagaaaacg
     3181 cgcccctgca ggattatggg cgacgtctaa aagcacgtgc cgctcgcgcc agggcaccat
     3241 ctccattcgc cccggcaggc tcgccctggc gagggtttgc aggaccagat cgccgtcaac
     3301 caccggggcc gcatcgtcag cggcattgca gaagccgatg gccagcacag cggcgcagag
     3361 gcccatggcg cagttctccg gagccagcgc tcccccaggc accaaaggca ggtccagccc
     3421 gagacccgcg aagctccaac cgccgccgct ttggcagcgg attcgatacc cgtgcccggg
     3481 ttctgcgata tcgcagccca gggcccgggc ccggtcgcga acggaatccg tgacgtcggc
     3541 cccgagcacc actttctggc caggccggaa gacgcccgcc ttctcccggc cgatggtttc
     3601 gagatcgtct cccaggaagc tctgatgatc gagcccgatg ctggtcacca ccgccacctg
     3661 cgcatccacg atgttgaacg catccaggcg ccccccgaga ccgacttcga gcacggccac
     3721 gtccaccgcc gcagatcgaa agacctccag cgccgccagg gcggcgaact cgaagtaggt
     3781 cagcggaacg tcgttacgcg ccgcttcgac cttctcgaac gcggcgacca gcgcagcatc
     3841 ttcgcaggga cgaccgttga ctcgaatccg ctcgttaaaa acgtgcagat ggggggataa
     3901 cgtggcccct accgacagac cagccccgag cagcacggcc tcggcgcagg tcgctgtgga
     3961 acccttcccg tttgtcccgg cgacgatgat cgagacgggt gcaggtttga ggacctccag
     4021 ccgggtggcg accgcccgca ccctgtcgag gcccagctcg atctgttgcg ggtgctgccc
     4081 ctcgatgtac ccaagccacc ctggcagcgt ggcgggcatt ccggctggcc ggtcaggcat
     4141 ccagggcacg ccggatctcg gccagctgag cggtgaggag agcgggtatg gcggccaggg
     4201 cgtccgcgct gccggccatt gtcctgacga gagcgcttcc aacaacgacc ccgtcggctt
     4261 tctcggcaac cgcgcgagcc gtcccgccgt ccttgatgcc aaagccgacc agcacgggca
     4321 gctcggtcat ctgcttcagc gccccgactt gcttcgagac cgacccggtt tcgaggttgt
     4381 cggcccccgt gatgcccttc agcgagacgt agtacacgaa gccgcctgcc tgctcggtaa
     4441 tcagcgccgc ccgctccggt gtcgtggtcg gggccacgag aaaaatgagg tcgatgtcct
     4501 tttccttgag cagaccgcgc aacgcgccgg cttcttccgg tggcaagttg accatgatca
     4561 aaccatcgac gcctgcctcg gcagccgctt cggcaaatgt ttcctccccc atggcgagca
     4621 cggagttcag gtaacccatg agaacgatcg gggtaaggtc atcgtctttg cggaatctgg
     4681 ccaccatctc cagcacggca cgcaacgtgg tccccttggc aagcgcccgc tctgaggacg
     4741 cctgaatcac gggcccttcg gcctccggat ccgagaaagg cacgccgagt tccagcacgt
     4801 cggctccgcc cgcgaccagc gcgtgcaggg ccggtaccgt gactgacgga tcgggatcgc
     4861 cagcggttat gaacgagacc agggctttgc ggccctggcc gcccagcgcg ctcagccttc
     4921 gggagagacg gctcacacct cgatcccgtc tatctcggcc accgtgagga tgtccttgtc
     4981 tccgcgcccg gacaggttga tcagcatgac ggcgtcacga tcgaaatctc gggcgtgccg
     5041 cgctgcccag gctatggcgt gactggtctc cagcgcaggc atgatgccct ctgtccggtt
     5101 cagcaggcga aagccggcca tggcttcgtc gtccgtgacg gtatcgtact cggcgcggcc
     5161 aacgtccttg aggaacgaat gctccggccc gacgccgggg tagtcgagcc cggccgatat
     5221 ggaatgcgtt tcgatgatct gaccatcatc gtcttccatg aggtaggtgc ggttgccgtg
     5281 gagcacgccc acccgcccgg cgctcagggg cgccgcgtgc ctgccggtct cgagacccaa
     5341 gccgcccgct tccacaccga ccatgcgtac gtcgacgtcg tcgacgaacg ggtagaaaag
     5401 accaatggcg ttggagccgc cccccacgca ggccacgagg acgtcgggca gccggccgag
     5461 catgctcaga gactgagccc gcgcttcctg cccgatcacc gcctggaagt cacggaccag
     5521 catgggatag ggatggggtc cggccaccgt gccgatgatg tagtgggtcg tatcgacgtt
     5581 ggtcacccaa tcccgcatgg cctcattgag cgcatccttc agggtgcgcg agccggagtt
     5641 gacggggacc acctccgccc cgagcagctt catccgatag acgttcaggc tctgcctttt
     5701 gacatcttcg gcccccatgt agacgcagca ttccaggccg agacgggcag cgacggtcgc
     5761 cgtcgcgacg ccgtgctggc cagcgccggt ctcggcgatg acgcgaggct tgcccatgcg
     5821 cttggcgaga agcgcctggc cgacggtgtt gttgatcttg tgggcgccgg tatggttgag
     5881 gtcctcgcgc ttgaggtaga tctgcgcccc acccagatcc cgggacagac gcgcggcatg
     5941 atagagaggg gttggccgtc caacgaagtg ctcgaggtcg tgcgcgagct cggcctgaaa
     6001 ctcgggatcc ttgcgtgcgc gctcgtaaag ggcctccagc tcgtccaggg cgtgcatgag
     6061 cgtctcggcg acgaagcggc cgccgtgctg gccgaagtga ccgcccacgg gctggtcgta
     6121 cttgttaaac cccttccctt ccgacacggc gcacctcgct gacaaatttc ctgacccgct
     6181 ccgggtcctt gcggcccttg atcccgccct cgacgccgct gctgacgtcc acaccgaacg
     6241 gccgcacacg gctgatggca tcggccacgt tctccggtgt caggccaccg gccagaacca
     6301 gcggcttggc accagcttcc ggccagcagg ctagatccag gcgtacgccc gtgccgccag
     6361 caacccccgg gacataggcg tccagaagca ggcagcaggc gcccggatac tcggcctcga
     6421 ggccggcgac gtccacgggg ccgctcacac gcgttgcctt catgtaattc accccgaacg
     6481 cctcgcaata ggatccgggc tcgtctccgt gaaactgaag cacgtccagg tcaacgtccc
     6541 gaaggacggc ttccacctca gcagcatccg cgtcaacgaa aagcccgatg aggctgagcg
     6601 acccggccac ctcccctgcg atctcacgag cgagcccggt ggaaacgaag cgagggctct
     6661 gccgcacgaa gatcaaccca agggcgtcgg cgccggcgtc cgcaacgcaa cgtgcgtcct
     6721 cgggccgcgc aatcccacaa atttttacct tggtcggcac gaccggatcc gggggaaaag
     6781 gggcgtagtc tagcggagac gtaactggcc ttctagacgc gtgcgagccc acccagcgag
     6841 cgcagcaagg gtgggggttc gccgggagga aaggcttgat ccgggtagcg aacgccaacg
     6901 aggtagagcc catgggcagg tgccgtcggc ccgacatgct ttcggtctct gccggcaagg
     6961 gtctcggcca accacccggc cggccggcgc ccttcgccga cgtcgcgaag agccgccgcg
     7021 atgttgcgca ccatgtgcag aagaaacgcg tttgcggcga tgtccagcac caccagggga
     7081 ccgtgacgat ggacggtgat ccgatgcaca catcggtgag ccgactcgga ctggcacccg
     7141 cttgctctga acgccgagaa atcgtgctcg ccaacgaggc tgcgcgacgc cgcgtgcatg
     7201 gcgtcgtcat cgaggtgccg cacccgcacg gcccaccgat ccagcgtcgg ggaggtcacg
     7261 tcgtcctcgt accacagata catgtagcgt cgcgccgtgg ccgagtagcg cggatggaat
     7321 cgaggatcga cttcccgcgc ccagcgcacc ttgacggatg gcggggtcag ggcgttcacc
     7381 cctttgaccc aggcctgaat cggccgctcg gcattggagg cgaaaccgac gacctgaccc
     7441 gtggcgtgaa ccccggcatc ggtgcgcccg gcggctgcaa ttctcagagg atgatcggca
     7501 atacgcgaaa gcgcctgctc cagggcaccc tgaaccgtag ctgccgcctt ctgcagctgg
     7561 aagccgtgaa aggcactgcc gtcatactcg acgcccagcg ccaggtgcat cagagctgtt
     7621 cgagcagcgt ctgcgctttt gtctgctggt cgctggtacc ttcctcgaga acttccttga
     7681 gaatgtcccg cgcgccgtcc tcgtcaccca tgtcgatgta ggccttcgcg agatccagct
     7741 tcgtctccgc gctgtcggcg tcgctttcga cgtcgaaatc gaactcatcc tcgtcgatgg
     7801 tggcgacttc gcttccttct tcgtcggcat ccagcgcggt cgcctcgggc ttgaagtcga
     7861 ttccgaggtc accgccgagc tgatcgccgg cgccgcggga tgcgccctcg tcctcatcga
     7921 gctccagatc gaactcgctc tcgccgccgt ccgcttcgac cgccgtcgtc gaggccggct
     7981 catcgtctag gccgagatcg aagtccatga cgttggactc gccttcgtcc tcagcagcct
     8041 ccgccttaac gggagcctcc tgag
//
LOCUS       DBNHLM010000333        12263 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_50303, whole genome shotgun sequence.
ACCESSION   DBNHLM010000333 DBNHLM010000000
VERSION     DBNHLM010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12263)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12263)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12263
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_50303"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..247)
                     /gene="csrA"
                     /locus_tag="AC16QF_15450"
     CDS             complement(56..247)
                     /gene="csrA"
                     /locus_tag="AC16QF_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HIR6450005.1"
                     /translation="MLILTRREGESLMIGDEISVTILSVKGNQVRLGVKAPKDVVVHR
                     EEVYQRPESRVSQGGRNNQ"
     gene            complement(373..1605)
                     /locus_tag="AC16QF_15455"
     CDS             complement(373..1605)
                     /locus_tag="AC16QF_15455"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR6450006.1"
                     /translation="MALIVQKYGGTSVGDVERIRAVAERVTKLRREGHDVVVVVSAMS
                     GETNRLEALGRSLAGRPNPREFDVLLASGEQVTVALLAIVLGEMRCPAVSYLGSQVQI
                     RTDNHFGKARIEHIDTHRLRQDLDAGRVPVIAGFQGIDLAGNLTTLGRGGSDTTAVAV
                     AAALGVDECQICTDVDGVYTADPRVVDDARRLDHVTFEEMLELASLGSKVLHPRSVEF
                     AGKYHVPLRVVSTFEDGPGTLISTELKSMEQPIVSGIAHARDEAKITMSGVPDVPGVA
                     SRILGPIGRAPIEVDMIVQNTGADGLTDFTFTVKRSDFEHALELLEPVRDELGARELA
                     GDNKIAKVSVVGVGMRSHAGVAMRMFDALAEENINIQMISTSEIKISIVVAERYLELA
                     VRCIHDEFGLGEPDASAE"
     gene            complement(1609..4215)
                     /gene="alaS"
                     /locus_tag="AC16QF_15460"
     CDS             complement(1609..4215)
                     /gene="alaS"
                     /locus_tag="AC16QF_15460"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR6450007.1"
                     /translation="MNTAEIRSAFLSFFAEREHRVVPSSSLVPANDPTLLFTNAGMNQ
                     FKDALLGREDLGYRRAATAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     EETIGWAWDLVTDVFAVPVEKLWVTVHTSDDESRAIWERKIGVPAERVISLEENFWAM
                     GDTGPCGPCSELFYDHGPEVAGGPPGSPDEDGDRYIEFWNLVFPQYDRQPDGELDLLP
                     QPGVDTGMGLERIAAILQGVHSNYEIDAFRSLLSAAGGLAGINDPRDCLTNASVRVIS
                     DHIRSSAFLIADGIMPGNEDRSYVLRRIIRRALRHGYKLGIREPFFHRLVKPLAEEMG
                     EAYPEIVERQADVARALLREEEKFAETLGHGMALLNQTIDDLTGSEIPGEVVFQLYDT
                     FGFPTDLTADVARERHLTVDVDGFDKAMKAQQERSKASARFSANVAQRIYTEGKVEFS
                     GYELAEEQSTVVGVFGAEGQAADALGAGENGIVVLDRTPFYAESGGQVGDTGVLQGDG
                     FQFQVTDTRPGGDQHLHIGSVTKGTVTVGQTVTARIDAQRRRAIVLNHSATHLMHAAL
                     RDELGSHVQQKGSLVAPDRLRFDFSHPEPMSREQLRRVEAAVNAQIRENSEVLAEVVG
                     YDDAINRGAMALFGEKYGDQVRVLTMGEGYSVELCGGTHVGRTGDIGVFRMVSESGVA
                     AGVRRIEAVTGRGALDWIDEGEEKLLAVGALVRSGRVDVEEKVAALLEENRRLARALE
                     QANEKLASAAGSDVAERAVDVKGVKVIAAQLDGDPKTMLQTLDTLKSKLGSCVVVLGN
                     VSGGKANLIVGVSKDLTDRITAPSILEVVGTKAGARGGGRPDMARAGGGDPDALPGAL
                     GSVADLIAQRLG"
     gene            complement(4333..4821)
                     /locus_tag="AC16QF_15465"
     CDS             complement(4333..4821)
                     /locus_tag="AC16QF_15465"
                     /inference="COORDINATES: protein motif:HMM:NF046475.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR6450008.1"
                     /translation="MLIEHKAVQEPDSAGDEQFQVAFARGLKLLATREHSAAELRRKL
                     RVKGFSEALAGDAVAALQDQGLQSDRRFAEVFVRSRLSKGYGPVRIRRDLYQRGIGDD
                     VIDEELTFPAERWVELATAVRAKRFPESEPTEHGVWNVQARFLSRKGFPADIIYRVLG
                     QV"
     gene            complement(4854..5942)
                     /gene="recA"
                     /locus_tag="AC16QF_15470"
     CDS             complement(4854..5942)
                     /gene="recA"
                     /locus_tag="AC16QF_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR6450009.1"
                     /translation="MANEQSNRKDSQADSQQDPQRSRALSAALAQIERQFGKGSMMRL
                     GDRELVAIPAIPTGSLGLDIALGIGGLPRGRVVEIYGPESSGKTTLTLQVIAEAQKGG
                     GTCAFIDAEHALDPIYADALGVDTDNLLVSQPDTGEQALEICDMIVRSGAVDVVVIDS
                     VAALTPKAEIEGEMGDAHVGLQARLMSQALRKMTGNIKQSNTLVIFINQIRMKIGVMF
                     GSPETTTGGNALKFYSSVRLDIRRIGAVKEGEEVVGNETRVKVVKNKVAPPFRQVEFQ
                     ILYGEGINHLGEVLELGVQQGIVEKSGAWYAYKSDRIGQGRKNACDYLESNPEIAAEI
                     EAQIREQLLPVRERSEDLVIPEPAQETL"
     gene            6076..8643
                     /gene="mutS"
                     /locus_tag="AC16QF_15475"
     CDS             6076..8643
                     /gene="mutS"
                     /locus_tag="AC16QF_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR6450010.1"
                     /translation="MNTGSKTNHTPMMQQYLSIKARHPDDLLFYRMGDFYELFYEDAE
                     RAAELLDITLTARGQSAGKPIPMCGVPYHAADGYLARLVRQGVRVAICEQIGDPATSK
                     GPVAREVQRIVTPGTLTEEALQDARRDSILMAINPGPDEVYGLALLNLAAGRLQVAEV
                     RTRPLLAAEVARSDPSEILAVDELPEAVTGQRHLTRRDPLEFDTDLGRAKLTRHFATR
                     SLEGFDVPEDSPMVGAAAAVLEYAKSSQCRDLAFIDAMHRVAADDVVVLDPHSRRNLE
                     IDRRVDGSEENTLFALFNTTQTPMGARLLRSWLAGPSRNAHTVEARQASVESLLGADN
                     DNLRRLLKEVGDLERIVARVALGSATPRDLSRLRTGLGQVPDVKDFLQTLEAPRLADL
                     MARLPDFNDEVELLGTAIVEAPPATIRDGGVIASGYDAQLDSLRNLSDNAAGWLADLE
                     LRERERTGIATLKVGYNRVHGYYIETSRAAGNEVPAEYVRRQTLKNAERYITPELKQF
                     EDEALTSQARALKLEKQLYQNLLEHLGARAGGSRDAAAILAELDVLSCFAERASALRL
                     VRPEFSADPIIDIRQGRHPLVEQASAKPFVANDLSLGEKVRMLIVTGPNMGGKSTYMR
                     QSALIVLLAFAGSYVPAEAARLGPIDRIFTRIGAADDLAGGRSTFMVEMTETANILRN
                     ATPRSLVLLDEIGRGTSTYDGLALAWATAEHLARETGAFTLFATHYFELTELPEELSQ
                     CRNVHLTAAEHDGKIVFLHSVEPGPASQSYGVQVARLAGVPNEVIARARTRLQSLEQQ
                     QAERIAQQPDLFEGQSAEPEAPSPLEARLSELDVDGLSPREALALLYELKGLASPDS"
     gene            8737..9060
                     /gene="fdxA"
                     /locus_tag="AC16QF_15480"
     CDS             8737..9060
                     /gene="fdxA"
                     /locus_tag="AC16QF_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HIR6450011.1"
                     /translation="MTFVIGENCIKCKLTDCVEVCPVDCFYEGPNMLVIHPDECIDCA
                     LCEPECPVDAIYSEDELPEDQQDFLEINREQAELWPNITEKKDPAEDYEDWIGKPDKR
                     HLLER"
     gene            complement(9077..9949)
                     /locus_tag="AC16QF_15485"
     CDS             complement(9077..9949)
                     /locus_tag="AC16QF_15485"
                     /inference="COORDINATES: protein motif:HMM:NF015951.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HIR6450012.1"
                     /translation="MSARGARQWSGLFARGMAMGVAEVVPGVSGGTIAFITGIYHELV
                     TTLAGFRPASARLLVTAGPVAFWQQHNLGFLAVLVAGMAASVLLFARLIGYWLDVVPT
                     LVWAFFFGLIAASVFQIGRGRPTRTLAVYGTIGLAAGASLLLVDPLPAQGAVWVFFFA
                     GMIAISAWLLPAISGSFILLILGLYEPVIEALNGFDWVVLTSLGLGCATGLMIFARAL
                     EWLLSYWREPLLSLLTGFMAGSLVRLWPWAQGGTLLTPAEYAAVTGQASLELWAVAAA
                     AAGALALWLLSRLE"
     gene            complement(9960..10595)
                     /locus_tag="AC16QF_15490"
     CDS             complement(9960..10595)
                     /locus_tag="AC16QF_15490"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR6450013.1"
                     /translation="MTSQRTRDRLVQRLAEHGIVDQEILDLIGHVPRHIFVDEALSHR
                     AYEDSALPIGHGQTISQPFVVAFMTSTLLNAPRARVLEVGTGSGYQTAVLRSSRRVRR
                     VFTVERLGPLLARAEERLKAMGLESVRYRHGDGYEGWPEEAPFDGILVTAAPRQVPEA
                     LLDQLAEGGRMVVPVGGGGVQELRVYDRTATGVNVDTVRHVRFVPLVKGLL"
     gene            complement(10622..11587)
                     /gene="truD"
                     /locus_tag="AC16QF_15495"
     CDS             complement(10622..11587)
                     /gene="truD"
                     /locus_tag="AC16QF_15495"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HIR6450014.1"
                     /translation="MTIDPHFGRLPLAVPGKRAKARVKATPEDFLVDELRAAAPSGAG
                     EHLYLHVEKRLLETRQVARRLAELYGVAAVDVGYAGMKDKRAVARQWLSVRTSLGPEV
                     AERGGVVVLAAGRDERKLRRGELAGNRFEIRLREVSGDDWRDRLGRVFESGAPNYFGP
                     QRFGGDNLDRALAWLPVRRRRSISPFRRGLYLSVLRSLLFNAVLARRVQEKTWRALLP
                     GDLDLDGMPTAPLWGRGRSATSGVALAAESAALAPFADVLDGLEHAGVDQARRSLVLL
                     PQNAWVAEEDEAVRLGFELPPGGYATSLLREAFELGEATDGAPAS"
     gene            complement(11577..11900)
                     /locus_tag="AC16QF_15500"
     CDS             complement(11577..11900)
                     /locus_tag="AC16QF_15500"
                     /inference="COORDINATES: protein motif:HMM:NF016839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HIR6450015.1"
                     /translation="MPLSTAVAASCSEPMATRLLIVVLAVLVALLQYRAWFGEVGYFA
                     AQELADEVDEQRRRGAELARRNELLTAEVVALKTGVDALEARARKDLGMIKRGETFYL
                     IPDDD"
     gene            complement(11863..>12263)
                     /gene="eno"
                     /locus_tag="AC16QF_15505"
     CDS             complement(11863..>12263)
                     /gene="eno"
                     /locus_tag="AC16QF_15505"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784437.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR6450016.1"
                     /translation="WDGWRALTDRVGDRIQLVGDDLFVTNTEILAEGIRRGVANAILI
                     KLNQIGTLSETLSTIRMAQEAGYRTVISHRSGETEDTTIADLAVATGAGQIKTGSLSR
                     SDRVAKYNRLLRIEEMLGDAAEYRGRGELL"
BASE COUNT         2274 a         4217 c         3702 g         2070 t
ORIGIN      
        1 cacctctccg ggagggccga agtttaccgg ccgacggcca ccgtagccag cgatgctact
       61 ggttgttgcg cccgccttgg gacacgcgtg attccggccg ctggtagact tcttcgcggt
      121 gaaccaccac gtctttcggc gctttgacgc cgagacggac ctgattacct ttgacgctga
      181 gaatcgtcac cgaaatctca tcgccgatca tcaggctttc accttcccgt cgggtcagta
      241 tcagcatgcc tgtgctccgc tgcccggcgt gctgtccttt ccctcgctcg cggcttatca
      301 attgaggctg atcgggcccg ctccatcgag ccgtgctaac cgtttcgcct tatcgtttcg
      361 cgattcgacg ccctattcgg cgctcgcgtc aggctcgccc agtccgaact catcatgaat
      421 gcagcgaacg gcaagctcaa gataccgctc cgctacgaca atggaaatct ttatctcgga
      481 agtcgatatc atctggatgt ttatgttctc ctcggccaga gcatcgaaca tccgcatggc
      541 gacgccggca tgggagcgca tgccgacacc caccacggac accttggcga tcttgttgtc
      601 accggcaagt tcccgcgcgc cgagctcgtc gcgcaccggc tccagaagct ccagagcgtg
      661 ctcgaagtcg gaccgcttga cggtgaacgt gaagtcggtg aggccatcag cgcccgtgtt
      721 ctgaacgatc atgtcgacct cgatgggggc acggccgatg ggcccgagaa ttctggaggc
      781 gacgccgggc acgtccggca ccccggacat ggtgatcttc gcttcatccc gagcatgcgc
      841 gatccccgat acgatgggct gttccatgct tttcaactcc gtactaatca gcgttcccgg
      901 tccatcttcg aaagtggaga caacccgcag aggaacgtgg tacttgccgg caaactccac
      961 cgagcgcgga tgcagaactt tcgagccgag gctcgcaagc tccagcatct cctcgaaagt
     1021 cacgtggtcg agtcggcggg catcgtcgac gaccctggga tcagccgtgt agacaccgtc
     1081 cacgtcagtg cagatctggc attcgtccac gcccagcgct gccgccacgg ccaccgccgt
     1141 cgtgtcagac ccgccccggc cgagggtcgt gaggttaccg gcaagatcga tgccctggaa
     1201 tcccgcaatc acgggcaccc ttccagcgtc gaggtcctgc ctgagccgat gcgtatcgat
     1261 gtgctcgatg cgcgccttac cgaaatgatt gtccgtccgg atctgcacct ggctacccag
     1321 gtacgagacg gcaggacacc tcatctcacc gagcacgatg gcaagcagcg caacggtcac
     1381 ctgctcgcct gaagccagca gaacgtcgaa ctcccgagga ttcggtcggc ccgccaggct
     1441 tcggccgagg gcttccagac gattggtttc cccgctcatt gcggacacga cgacgaccac
     1501 gtcgtggccc tcccgccgca gcttcgtaac ccgctcggcg acggcccgga tgcgctccac
     1561 gtcacccaca ctggtgccgc cgtatttctg aacgatcaga gccatggttc agcccagtcg
     1621 ttgcgcgatc agatcggcaa cgctcccaag cgccccaggc agagcgtctg ggtcaccacc
     1681 gcccgctctg gccatatcgg gccgcccgcc accccgggcg ccagccttgg tccccaccac
     1741 ctccagaatg ctcggggcgg tgatgcgatc cgtcagatcc ttgctgaccc cgacgatgag
     1801 gtttgccttg ccgccagaca cgttgccaag caccaccacg cagcttccga gcttggattt
     1861 gagggtgtcc agggtctgca gcatcgtctt cgggtcaccg tcgagctgcg cggcgatcac
     1921 tttcactccc ttgacgtcca ccgcacgctc ggcgacgtcc gagcctgcgg cagacgcgag
     1981 cttctcgttg gcctgctcca gcgcacgggc aagacggcgg ttctcctcca gcagcgcggc
     2041 cactttctcc tccacgtcaa cccggccgct cctgaccaga gcacccaccg ccagcagctt
     2101 ctcctcgccc tcgtcgatcc agtccagggc accgcgaccg gtgacggcct cgattcgcct
     2161 cacacccgcg gccacgccgg attcgctgac catccggaag acgccgatgt cgccggtccg
     2221 accgacgtgg gtgccaccgc agagctccac cgaatagcct tcgcccatgg tgagaacccg
     2281 gacctgatcc ccgtactttt ctccgaaaag agccatggcg ccgcgattga tggcgtcgtc
     2341 gtacccgacc acctcggcca gcacttcgct gttctcccgg atctgcgcgt tgacggcggc
     2401 ttcgacgcgc cggagctgct cccggctcat cggttcggga tgggaaaagt cgaacctcag
     2461 ccgatccggc gccaccagag atcctttctg ctggacgtgg ctcccgagct catcgcgaag
     2521 agcggcgtgc atgaggtgag tggcagagtg gttgagaacg atggcacgac ggcgctgggc
     2581 gtcgattcgc gccgttaccg tctggccgac cgtcaccgtg cctttcgtga ccgaaccgat
     2641 gtgaaggtgc tgatccccac cgggccgggt atccgtcacc tgaaactgga acccgtcacc
     2701 ctgcaacacg cccgtatcgc ccacctgacc gcccgactcc gcataaaacg gggtccgatc
     2761 gagaacgacg atgccgttct ctcctgcccc cagcgcatcg gcagcctggc cttccgcacc
     2821 gaacacgcct accacggtgg attgctcctc cgcgagctcg tagccggaaa actccacctt
     2881 tccttcggtg tagatcctct gcgcaacgtt ggcggagaac cgtgccgacg ccttgcttct
     2941 ctcctgctgc gctttcatgg ccttgtcgaa gccgtccacg tccacggtca ggtggcgctc
     3001 tctggcaacg tccgccgtga ggtccgtcgg gaagccgaaa gtgtcgtaga gctggaagac
     3061 cacttcgccg ggaatctcgc tgcccgtcag gtcgtcgatg gtctggttga gcagcgccat
     3121 gccgtgaccg agggtctcgg caaacttttc ctcctctcgc agcagagccc tggctacatc
     3181 ggcctgtcgc tccacgatct cgggatacgc ctcgcccatc tcctccgcga gcggcttgac
     3241 gagcctgtgg aagaacggct cccggattcc cagcttgtat ccgtgtcgca gcgcgcgacg
     3301 aatgatccgc cgcagaacgt agctgcgatc ctcgttgcca ggcatgatcc catcggcaat
     3361 cagaaaagcg ctcgagcgta tgtggtcgga tatcacccgc acggaggcgt tggtcagaca
     3421 gtcacgcgga tcgttgatgc ccgcgagccc gcctgctgcg ctgagcagcg accggaaagc
     3481 gtcgatctcg tagttgctgt gcaccccctg caggatggcc gcgatgcgct cgagccccat
     3541 gccggtgtct acacccggct gcggaaggag atccagttct ccatcgggct gacggtcgta
     3601 ctgcggaaac accaggttcc agaactcgat gtaacgatcc ccatcctcgt cgggagatcc
     3661 cggcggccca ccggccacct cgggcccgtg atcgtaaaac agctcgctgc aaggcccgca
     3721 gggtcccgtg tcacccatgg cccagaagtt ctcttccagc gagatgaccc gctccgcggg
     3781 aacgccgatt ttccgctccc agatggccct cgactcgtca tccgaggtgt gaacggtcac
     3841 ccacagcttc tctacgggaa cagcaaatac gtccgtgacg agatcccacg cccagccgat
     3901 ggtctcttcc ttgaagtagt cgccaaaact gaagtttccg agcatctcga aaaacgtgtg
     3961 gtggcgagcc gtgtatccga cgttttccag atcgttgtgc ttaccacccg ccctgacgca
     4021 acgctgagcc gtcgccgcgc gacgatagcc tagatcctcg cgaccgagca gcgcgtcctt
     4081 gaactggttc attccggcgt tggtgaaaag caaggtcgga tcgttggccg gcaccaggga
     4141 actcgacggc acgacgcggt gctcccgctc ggcgaagaac gacaggaatg ctgaacggat
     4201 ttcagcggtg ttcatctttg gaatcaacca attaggaggc gcgcagtata cggaaaccgc
     4261 gtccaattga catggccgcc tggtctgcca cggggtgacg gacagggcct ccgcggcggc
     4321 gagattgggc gcctagacct gccccagcac ccggtaaatg atgtcggccg gaaacccctt
     4381 gcgggacaga aaacgcgcct gcacattcca gacgccgtgc tctgtgggct cgctttccgg
     4441 aaaccgtttg gcccgaaccg cagtggcgag ttcgacccag cgttccgccg ggaaggtcag
     4501 ctcctcgtca atcacgtcat cgccgatgcc gcgttgatat agatcccgac gaattcggac
     4561 gggcccgtac cccttgctga gacgactgcg aacaaacacc tccgcgaagc gccggtcgga
     4621 ttgcagcccc tggtcctgca gcgcagcgac ggcgtctccg gccaacgcct cagaaaatcc
     4681 tttgacgcga agcttgcgtc gaagctcggc cgccgaatgc tcccgggtgg cgagcagctt
     4741 gaggcctcgg gcaaacgcca cctggaactg ctcatcgccc gcgctgtccg gctcctgaac
     4801 cgccttgtgc tcaatcaaca tgcgaccacc gcgctccccg gggcctgatt cgtctacagc
     4861 gtttcctggg ccggctcggg aatgacgaga tcctcggaac gctctctaac gggcaggagc
     4921 tgctcgcgaa tctgggcttc gatctcggcc gcgatttcag gattggactc gaggtagtcg
     4981 caggcgttct tccgtccctg cccgatccga tcggacttgt aggcatacca ggcacccgat
     5041 ttctcgacga tgccctgctg cacacccaac tccaggacct caccgaggtg attgatgccc
     5101 tccccgtaaa ggatctggaa ctcgacctgc ctgaagggcg gagccacctt gtttttgacc
     5161 actttgaccc gagtctcgtt gccgaccacc tcttcgcctt ccttcacggc gccgatccgg
     5221 cggatgtcca gccgcaccga cgagtaaaat ttcagcgcgt tcccgccggt ggtggtttcc
     5281 ggagaaccga acatgacccc gatcttcata cgaatctgat tgatgaagat gaccagggtg
     5341 ttcgactgct tgatgttgcc cgtcatcttt cgaagcgcct ggctcatcag acgagcctgc
     5401 aggccgacgt gggcatcccc catttcccct tcgatctccg ccttgggggt cagtgccgcc
     5461 acggagtcga tgaccaccac gtccacggct ccggaacgga cgatcatgtc gcagatttcc
     5521 agagcctgct cgccggtatc cggctgggat accagcagat tgtccgtatc gacaccgagc
     5581 gcatcggcgt agatggggtc gagggcgtgc tccgcgtcga tgaaagcgca ggtgccgccg
     5641 cccttttgag cctcggcaat gacctggagg gtgagcgtcg tcttacctga cgactccgga
     5701 ccgtagatct cgaccacccg gccccttggc agaccgccga tacccagagc aatgtctagc
     5761 cccagcgagc ccgttgggat cgccggtatg gcgaccagct cccgatctcc aagccgcatc
     5821 atcgagcctt tgccgaactg gcgctcgatc tgcgccagcg ccgcggacag cgcccgggat
     5881 ctctgcggat cctgctggga atccgcctgc gaatctttgc ggttggactg ctcgttggcc
     5941 atgaatctct cctgcggggt taacggacgg tactgtatat ataatcagta ttgatggcaa
     6001 ccgctcgaag ggcccttcta tacgttctcg cacatgccct tgagacatct cggagccaca
     6061 gagaataata ggttcatgaa cacgggatcc aagaccaacc acacgccgat gatgcagcag
     6121 tacctgagca tcaaggcccg gcatccggac gacctgctgt tctaccgcat gggcgatttc
     6181 tacgagcttt tctacgagga tgccgagcgc gcagccgagc tgctggacat cacgctcact
     6241 gcccgtggcc agtcggcggg caagccgata cccatgtgtg gcgtgcccta ccacgcggct
     6301 gacgggtacc tcgcccggtt ggtgcgccag ggcgtgcggg tggccatctg cgagcagatc
     6361 ggcgacccgg ccaccagcaa gggaccggtg gccagagagg tccagagaat agtcaccccc
     6421 ggcaccctca ccgaggaggc tctccaggac gcccgacgcg acagcatcct catggccatc
     6481 aatccgggcc cggacgaggt ctacggcctg gcgctgttga accttgcggc cggcaggctg
     6541 caggtcgcag aggtgcggac ccgcccgctg ctggcagccg aggtggcccg cagcgatccg
     6601 agcgagatct tggccgtcga cgagttgccc gaagcggtca ccggacagcg ccacctgacg
     6661 cggcgcgatc cgctggagtt cgacaccgat ctcggccggg ccaagctgac cagacacttc
     6721 gcgacgcgct ccctggaggg cttcgacgtc cccgaggact cgcccatggt gggcgccgcg
     6781 gccgccgtgc tcgagtacgc aaagtcctcg cagtgtcgcg atctggcgtt catcgacgcc
     6841 atgcatcgcg tggctgcgga cgatgtggtc gtgctggatc cgcactcacg gcgcaatctg
     6901 gaaatcgatc ggcgcgtgga cggcagcgag gagaacacgc ttttcgctct gttcaacacg
     6961 acgcaaacgc ccatgggagc acgcctgctg cgaagctggc tcgccgggcc cagccgcaac
     7021 gcccacaccg tcgaagcgcg ccaggcctcg gtcgaaagcc tgctgggtgc ggacaacgac
     7081 aacctgcgcc gactgctcaa ggaggtcggc gacctggagc gaatcgtcgc ccgggtggcg
     7141 ctgggttcgg cgacgccgcg cgacctgtca cggctgcgca cgggcctggg acaggtgccg
     7201 gacgtgaaag acttcctgca gacgctcgaa gccccccggc tcgccgatct gatggctcgc
     7261 ctgcccgact tcaatgacga ggtcgagctg ctcgggaccg cgatcgtcga ggcgcctccg
     7321 gccacgattc gagatggcgg cgtgatcgcc tccggctacg acgcccaact cgatagcctg
     7381 cggaatttga gtgacaacgc cgccggctgg ctggccgatc tcgagctgcg cgagcgggag
     7441 cggacgggca tcgcgaccct gaaggtcggc tacaaccggg tccacggcta ctacatcgag
     7501 acgagccgcg cggccggcaa cgaggttccg gccgagtacg tccgcagaca gaccctgaaa
     7561 aacgccgagc gctacattac cccggagctc aaacagttcg aggacgaagc gctcaccagc
     7621 caggcacgag cgctcaagct tgaaaagcag ctgtaccaga acctgctgga gcacctgggc
     7681 gcaagagccg gcggttcgcg cgatgccgcg gccatcctcg cggagctgga cgtgctctcg
     7741 tgcttcgccg agcgcgcaag cgccctgagg ctggtgcgac ccgagttctc ggcggacccc
     7801 atcatcgaca tcaggcaggg ccgccatccg ctcgtcgagc aggcgagcgc aaagcccttc
     7861 gtagccaacg acctctcgct gggtgagaag gtacggatgc tcatcgtgac ggggcccaac
     7921 atgggcggca agtctacgta catgaggcaa tcggccctca tcgtgctgct cgctttcgcc
     7981 ggcagctacg tcccggccga agccgcgcgg ctaggcccca tcgaccgcat cttcacccgc
     8041 atcggcgcgg ccgacgacct cgccggcggt cgctctacct tcatggtgga gatgaccgaa
     8101 accgcgaaca ttctgagaaa cgccacgccg cgcagcctcg tcctgctcga cgagatcggt
     8161 cgcgggacca gcacgtatga cgggctggca ctggcctggg caaccgccga gcatctggcc
     8221 cgggaaacgg gcgcgttcac cctgttcgca acgcactact tcgagctgac ggagctgccc
     8281 gaggagcttt cccagtgccg caacgttcat ctgaccgctg ccgagcacga cgggaagatc
     8341 gtcttcctgc acagcgtcga gccagggccc gccagccaga gctacggtgt gcaggtggcc
     8401 cgacttgccg gcgtgcccaa cgaggtcatc gcccgcgcgc gaactcgcct gcagtcgctg
     8461 gagcaacagc aggcagagcg tatcgcgcag caacccgatc tcttcgaggg acaatccgcg
     8521 gaacccgagg cgccgagtcc cctcgaagcg aggctgtccg agctggacgt agacgggttg
     8581 agccctcggg aagccctcgc gctgctctac gaactcaagg gtttggcgtc ccccgatagc
     8641 tgacgggcgc ccgctcgaca ggggatcgaa tatggttaga atacggtccc ttcgcagacc
     8701 cgaaacacca acttacccga caggaaggca atacagatga cgttcgtcat tggggaaaac
     8761 tgcatcaagt gcaagctcac agactgcgtt gaggtatgcc cggtagactg cttctacgaa
     8821 gggccaaaca tgctcgtcat ccatccggac gaatgcatcg actgcgccct ttgcgagccc
     8881 gaatgcccgg tggatgccat ttactcggaa gacgagcttc ccgaggatca gcaggacttc
     8941 ctggagatca accgcgagca ggccgagctg tggccgaaca tcaccgagaa gaaggatcct
     9001 gcggaagact acgaggactg gatcggcaaa cccgacaagc gccatctcct cgaacgctga
     9061 ccgattcggc ctgcgtctac tccagcctgg acaacagcca gagggccaat gcgcccgcag
     9121 cggccgcagc aaccgcccac agttccagac tcgcctgacc cgtcaccgca gcatactcgg
     9181 ccggcgtcag cagggtgccg ccttgggccc agggccacag gcgcaccagc gagcccgcca
     9241 tgaatcccgt cagtagagaa agcaacggct cgcgccagta ggagagcagc cactccagcg
     9301 cccgggcgaa gatcataagc ccggtggcac atccgagacc cagactcgtg agcaccaccc
     9361 agtcgaaccc gttcagcgcc tcgatgacgg gctcatagag cccgagaatc agcaggatga
     9421 agctacccga gatagcgggg agcagccatg cagagatggc gatcatcccg gcaaagaaga
     9481 agacccaaac ggccccttgg gcgggcaacg ggtcgacgag caggagcgat gccccggcag
     9541 cgagcccgat ggtgccgtac acggcaagcg tacgcgtcgg gcgaccgcgg ccgatctgga
     9601 acaccgaagc ggcgatgagc ccgaagaaga aagcccagac cagggtcggc accacgtcga
     9661 gccagtagcc gatgagccgg gcgaagagca gaacgctggc agccataccc gccaccagca
     9721 cggccagaaa ccccagattg tgctgctgcc agaacgcaac cggtcctgcg gtcacgagca
     9781 gacgcgcgga agcgggcctg aacccggcca gggtggtcac gagctcgtgg tagatgcccg
     9841 tgatgaaggc aatggtgcct ccggacacac cgggaaccac ctcggcgacg cccatggcca
     9901 tgccgcgcgc gaagagaccg ctccactgac gggcgccgcg ggcgctcacg ctgcgaccct
     9961 catagcaggc ccttgaccag cggcacgaag cgtacgtgcc tcaccgtgtc cacgttgacg
    10021 ccggtggcag tgcgatcgta aacgcgcagt tcctgcacgc caccgccgcc cacgggcacc
    10081 accatcctcc cgccttccgc cagctgatcc agcagagctt ccggaacctg ccgcggtgct
    10141 gccgtcacga gaatgccgtc gaacggggct tcctccggcc atccttcgta accgtcgcca
    10201 tggcggtacc tgaccgattc cagccccatg gccttcaagc gctcttcggc gcgggcgagc
    10261 agcggcccga gacgctcgac ggtgaagacc cggcgaacgc gccggctcga tcgcagcacg
    10321 gccgtctggt atccggaccc ggtccctact tccagaaccc gcgcacgtgg cgcgttcagc
    10381 agcgtggagg tcatgaaggc gacgacgaag ggctgcgaga tggtctgccc gtggccgatg
    10441 ggcagggccg aatcctcgta cgcgcggtgg gacagcgctt cgtccacgaa gatgtgccgg
    10501 ggcacgtggc cgatcaggtc gagtatttcc tgatcgacga tgccgtgctc ggccagtcgc
    10561 tgcacgagcc gatcccgggt gcgctgagag gtcatgccga tgccgaccag atcgtagccc
    10621 gtcacgacgc cggtgctcca tccgtggcct cgcccagctc gaaagcctcg cgcagcaggc
    10681 tggtcgcata tccgccaggg ggcagttcga agccgaggcg cacagcctcg tcttcctccg
    10741 ccacccaggc gttctgcggc aacagcacca gggatcgcct ggcctgatcc acccccgcat
    10801 gctcgaggcc gtctagcaca tccgcgaacg gggcgagcgc ggcgctctcc gccgccaggg
    10861 ccacgccgct ggtcgcagac cgaccccggc cccagagggg ggctgtgggc attccgtcca
    10921 gatccaagtc gcccgggagc agtgcccgcc aggttttctc ctgcacccgc cgggccagaa
    10981 ccgcgttgaa gagcaggctg cggagcacgg acaggtagag acccctcctg aacggcgaga
    11041 tggaccggcg gcggcgtacg ggaagccagg ctagggcgcg atcgagattg tcgcctccaa
    11101 aacgctgggg cccgaagtag ttcggcgctc ccgactcgaa gacgcgcccg aggcggtcgc
    11161 gccagtcgtc accggacacc tcgcgcaacc ggatctcgaa ccggtttccg gcgagctcac
    11221 cccgacgaag cttgcgctca tcacgacctg cggcaagcac gaccacgccg ccccgttccg
    11281 ccacctcggg gccgagagac gtgcgcacgc tcaaccactg acgggcaacg gcgcgcttgt
    11341 ccttcattcc ggcatagccc acatccacgg ccgcgacccc gtaaagctcg gccagacgac
    11401 gggccacctg ccgcgtttcc aagaggcgct tctccacgtg gaggtagaga tgctcgcccg
    11461 cgccgctcgg cgccgcggct cgcagctcgt cgaccaggaa gtcttccgga gtcgccttga
    11521 cacgggcctt ggcccgcttt cccggtacgg ccaagggcag acggccgaaa tgcggatcaa
    11581 tcgtcatccg gaatcagata aaaagtttca ccccgcttga tcatgcccag atccttgcgt
    11641 gcccgggcct cgagcgcgtc tacgcccgtc ttgagcgcca ccacttccgc cgtcagcagc
    11701 tcgttgcgcc gcgccagctc cgcaccccgg cggcgctgct cgtccacctc gtccgccagc
    11761 tcctgcgcgg cgaagtaacc aacctcgccg aaccatgccc ggtactgcag gagggcgacc
    11821 agaacggcca gaacgacgat gagcaaccgc gtcgccatgg gctcagagca gctcgccgcg
    11881 accgcggtac tcagcggcat cgccgagcat ctcctcgatg cgcagcaggc ggttgtattt
    11941 tgccacccga tccgaacggc tcagggagcc ggtcttgatc tggcccgcgc cggtggcaac
    12001 ggccagatcg gcaatggtgg tgtcctccgt ttcacccgac cggtgggata tcacggtgcg
    12061 atatcctgcc tcttgagcca ttctgatcgt gctgagggtt tcgctcagag tcccgatctg
    12121 gttgagcttg atcaggatcg cgtttgccac cccccggcgt atgccctcgg caaggatctc
    12181 cgtgttcgtg acgaacagat cgtcgcccac gagctgaatc cgatcgccga cccgatcggt
    12241 cagcgcgcgc cagccgtccc agt
//
LOCUS       DBNHLM010000334         2607 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_589365, whole genome shotgun sequence.
ACCESSION   DBNHLM010000334 DBNHLM010000000
VERSION     DBNHLM010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_589365"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1684
                     /locus_tag="AC16QF_15510"
     CDS             <1..1684
                     /locus_tag="AC16QF_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450017.1"
                     /translation="TATLICRGLFLSLLILAGCQPGDSAGEGSEPATVIDDAAALPAG
                     NSVAPLLEGIGPVDFPISTTSERAQAYFNQALTFSYGFNHAEAARSFREAARLDDTCG
                     ICWWGVALAHGPNINKEMTSEDVPVAWEALRNALARQHHETARERAYIDALTTRYTAD
                     GADRPTLDQEYAQAMEDVVRDYPDDLNAQALYAEALMDLYPWDYWDDDGSPRMHTGAI
                     VAALEKVMEADPDHPGALHLYIHVMERFTPELAEPAADRLGPLVPVAGHLVHMPSHIY
                     LRVGRYHDAVAANRRAALADEDYIAQCNAQGFYPAAYYPHNIHFLWYSAMMEGRRALA
                     VESAEKLRSKVPVDMARQFGSIQAYLAVPSYTFVRFGMWQEALAVPEPEEDLVIAAFM
                     RHYGRGVAHAALDDTGSAKAELAAMEAILASGEFRAQMDRQGDIGDILASIASSLVRA
                     RIARADGDVDAEIRHLRDAVASQDSLPYSEPPYWHYPVRQSLGSALLRLGDADGAAAA
                     FTEDLGKFPKNGWSLHGLAEARRALGKPTDELEAAHREAWQYADIEPTAGAE"
     gene            1709..>2607
                     /locus_tag="AC16QF_15515"
     CDS             1709..>2607
                     /locus_tag="AC16QF_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450 family protein"
                     /protein_id="HIR6450018.1"
                     /translation="MSEALAPIDLTTADPYSVPLDQIDMSQAALFQTNTFGPYFERLR
                     REDPVHYTADSFFGPYWSITRFDDIVYVDKHHELFSSEDGITLADRQQDFATVNFIAM
                     DPPKHDDQRKAVQGVVAPRNLAKMEALIRGRVADILDSLPLGETFNWVDRVSIELTTQ
                     MLATLFDFPFEERRKLTYWSDMATSSELAGGPTPEPVRREALMECLEAFTRIWHERAS
                     QPPDESVDLISMLAHSDDTKDMLNRPMEYLGNLILLIVGGNDTTRNSISGGVLALNQH
                     PKEYEKLRSDPSVIPSMVDEIIR"
BASE COUNT          488 a          917 c          797 g          405 t
ORIGIN      
        1 gaccgcaacg ctcatctgca ggggcctgtt cctcagcctg ctgattctcg ccggctgcca
       61 gcccggggac tcggcgggcg aaggttccga accggccacc gtgatcgatg atgcagctgc
      121 gctgcccgcc gggaacagcg tggcacccct gctcgaaggc atcggacccg tcgactttcc
      181 catcagtacc acctcggagc gcgcccaggc ctatttcaac caggcgctca cgttcagcta
      241 cggtttcaac cacgcagaag cggcccgctc gttccgggaa gccgcccggc tcgacgacac
      301 ctgcgggatc tgctggtggg gcgtcgcgct ggcccacgga cccaacatca acaaggaaat
      361 gacatccgaa gacgtgcccg tagcctggga agcgctgcgc aacgccctgg cgcgccagca
      421 tcacgaaacg gcccgcgagc gggcttacat cgacgcgctc accacgcgct acacggccga
      481 cggcgccgat cggcccaccc tggatcagga atacgcccaa gcaatggagg acgtggtgcg
      541 cgactatccc gacgacttaa acgcccaggc gctctacgcc gaggcgctga tggatcttta
      601 cccctgggat tactgggacg acgacggatc cccccggatg cacacgggtg ccatcgttgc
      661 cgccctggaa aaggtcatgg aggccgatcc cgatcatcct ggcgcattgc acctgtacat
      721 acacgtgatg gagcggttca caccggagct tgccgagccc gccgccgacc gccttggtcc
      781 gctggttccc gtggccggcc acctggtgca catgccgtcg cacatctatc tgcgcgtcgg
      841 ccgctaccac gacgccgtag ccgccaaccg ccgggccgcc ctggcagacg aggactacat
      901 tgcccagtgc aacgcccagg gcttctaccc ggccgcctac tacccgcaca acatccattt
      961 cctgtggtac tcggccatga tggaaggccg gcgggcactg gccgtcgagt cagcggagaa
     1021 gctgcgcagc aaggtgcccg tcgacatggc cagacagttc gggtccatcc aggcctacct
     1081 cgcggtgccg tcctacacct tcgtgcgctt cggcatgtgg caggaagctc tggcggttcc
     1141 ggaacccgag gaagatctgg tgatcgccgc gttcatgcgt cactacggcc gcggggtcgc
     1201 tcacgccgcc ctggacgata cggggtccgc aaaggcggag ctcgcggcca tggaggcaat
     1261 cctggcatcc ggcgagttcc gggcccagat ggatcgccag ggagacatcg gggacatcct
     1321 cgccagcatc gcctcgtccc tcgtgcgcgc ccggatcgca agagcagacg gggacgtgga
     1381 cgcggagatt cggcatctgc gcgacgccgt cgcaagccag gactcgctcc cgtacagcga
     1441 gccgccctac tggcactacc ccgtccgcca atcgctgggc tccgcgctgc tccggctggg
     1501 cgatgctgac ggggcagcgg cggccttcac cgaggatctc ggcaagtttc cgaagaacgg
     1561 ctggtccctg cacgggctgg ccgaagcccg ccgcgctctg ggaaagccaa ccgacgaact
     1621 ggaggcggcc caccgggaag cgtggcaata tgccgatatt gagccaacgg caggcgccga
     1681 gtagcgagtt caaaaccgga ggttcgtgat gtccgaagcc ctagcaccga tcgatctgac
     1741 cacagcggac ccgtactcgg tgcccctgga tcagatcgac atgagccagg ccgccctctt
     1801 ccagaccaac accttcggcc cgtacttcga gcgcctgcgc cgggaagatc cggtgcacta
     1861 caccgcggac agttttttcg ggccttactg gtccatcacc cgcttcgacg acatcgtcta
     1921 cgtggacaag caccacgagc tcttctcgtc cgaggacggc atcaccctgg ccgaccgcca
     1981 gcaggacttt gccaccgtca acttcatcgc catggatccg cccaagcacg acgatcagcg
     2041 aaaggccgtc cagggcgtcg tggcgccgcg caacctggcc aagatggaag cgctcatccg
     2101 cggacgcgtc gccgacattc tcgattcgct gccgctgggt gagaccttca actgggtcga
     2161 ccgggtttcc atcgagctca ccacccagat gctggccacg ctgttcgact tccccttcga
     2221 ggagcggcgc aagctcacct actggtcgga catggccacg tccagcgagc tggccggcgg
     2281 cccaacgccc gagccagtgc ggcgtgaggc gctgatggaa tgtctggaag ccttcacccg
     2341 aatctggcac gagcgcgcaa gccagccgcc ggacgagagc gtggatctga tctccatgct
     2401 cgcccacagc gacgacacga aggacatgct caatcggccg atggaatacc tcggcaacct
     2461 gatcctgctg atcgtgggcg gcaacgacac cacgcgcaac tccatctccg gcggcgtgct
     2521 ggcgctgaac caacacccga aggagtacga gaagctccgc agcgacccga gcgtgatccc
     2581 cagcatggtc gacgagatca tccgctg
//
LOCUS       DBNHLM010000335         2096 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1547528, whole genome shotgun sequence.
ACCESSION   DBNHLM010000335 DBNHLM010000000
VERSION     DBNHLM010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1547528"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            376..1593
                     /locus_tag="AC16QF_15520"
     CDS             376..1593
                     /locus_tag="AC16QF_15520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6450019.1"
                     /translation="MRTTYLTAGAIALAIVVWLLSGQMASGDPVRHQTLAENNAEALA
                     RTQDKAPTRVRARVMNATPQFQHVVLRGKTESKRVVEVRSETSGRVIERPVERGAYVT
                     QGDLLCRLAMDDREAALAEARESLNQARIEYEGSLKLKQRGFQSDTAIAQTKARLAAA
                     EAKLARSALEVSRTEIRAPFDGVVETIHQDVGDYVNVSAPCVTVVDADPMLLIGRVAE
                     KDVHRLLLDQNATGLLSNGTTVTGPVTFIGQQSDPATRTYAVEIQIPNPERAIRSGIT
                     TEIRIPVAETMAQKVSPAVFTLDDAGHIGIRIVNADYRVELHPVTIIREAPDGVWVSG
                     LPNVATVITVGQELVVAGEMVEVDFEPAATLPAAAPAERAVAPEADGPDSGPSAANLT
                     SRTSSTEALSRAT"
     gene            1590..>2096
                     /locus_tag="AC16QF_15525"
     CDS             1590..>2096
                     /locus_tag="AC16QF_15525"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6450020.1"
                     /translation="MNSLIDAAISRSRTTLLVMFMVVIAGLIARGAIPIASEPRIEVP
                     FFFVGVVHEGISPEDAERMLVMPMEIELRKVEGVKELTAYASEGSATILVEFDVSHDL
                     DQALLDVREAVDRARPELPSTAEEPYVREETTEDFPIVQVNLVGEGVPERILYNLAIE
                     LRDDIEAIP"
BASE COUNT          401 a          715 c          640 g          340 t
ORIGIN      
        1 agaagccggc caggtctgga tcgaggacga cgagatcggc ttcgacaacc tggaagcggc
       61 cggcagcctg tttcttggtc tggacagtcc catagggccc gtttttctcg ccgggggcat
      121 ggccgaaggg gggaacaagt ccatctacgt cttcctcggg caaccgtttt agccgcaagg
      181 ccgcctttag agccacatcg cgcggcgttt tcacgccgcc tacacaccgc catcgctacc
      241 tttaaccctg acagcttcca ggcccgccga gcatccttga ttgtgcagcg gcccatggaa
      301 ataccaccgg cagtttgtta ctttaggtaa cgtattgggt gctttagtaa caccacttgc
      361 tcatcggaga ccaaagtgcg aaccacatat ctcaccgcag gcgccatcgc gctggccatc
      421 gttgtctggc tgctttcggg ccagatggcg agcggcgacc ctgtcaggca tcagacgctg
      481 gcagagaaca acgcggaagc cctcgcgcgg acccaggaca aggcaccgac cagggtgcgc
      541 gcccgggtaa tgaacgccac gccgcagttt cagcacgtcg tcttgagggg gaaaaccgaa
      601 tcaaagcgcg tcgtcgaggt ccgctccgag acgtccggcc gcgtgatcga gagaccagtc
      661 gagcgcggtg cgtacgttac ccagggggat ctcctgtgcc ggcttgccat ggacgatcgc
      721 gaggctgcac tggccgaggc ccgcgaatcc ctcaatcagg cccggatcga gtatgaaggg
      781 agcctgaagc tcaagcagcg gggctttcag tccgataccg ccatcgccca gaccaaggcg
      841 cgtctagcag ccgccgaagc caagctcgcc cgaagcgctc tggaggtctc ccgcacggag
      901 atccgcgcgc ccttcgacgg ggtggtggaa accatacacc aggacgtggg cgactacgtc
      961 aacgtgagcg cgccgtgcgt caccgtcgtc gatgccgacc ccatgctttt gatcgggcgg
     1021 gtcgccgaga aagacgtgca ccggctcctt ctggaccaga acgcgaccgg gctgttgagt
     1081 aacgggacga ccgtcaccgg ccccgtcacc ttcatcggcc agcagagcga tcccgctacc
     1141 cgcacctacg cggtggagat ccagattccc aatccggagc gggcgatccg ctccggaatc
     1201 accaccgaaa ttcgcatccc cgtggccgag accatggccc agaaagtcag ccctgccgtc
     1261 ttcacgctcg acgatgcggg gcacatcggc attcgcatcg tcaacgccga ctaccgcgtc
     1321 gagcttcacc ccgtgaccat catccgagaa gcgccagacg gcgtgtgggt gtccggcctt
     1381 cccaacgtcg ccaccgtgat aactgtcggc caggagctgg tcgtggccgg agagatggtg
     1441 gaggtagact tcgagcctgc cgccaccctg ccggccgccg cccccgcgga gcgcgccgtc
     1501 gcgccggaag ccgacggccc cgacagcgga ccgtcagccg ccaacctcac cagccggacg
     1561 agctcgacag aagccctctc acgcgcgaca tgaacagcct gatcgacgcg gccatctccc
     1621 ggtcgcgcac cactctcctg gtcatgttca tggtcgtgat cgcgggcctg atcgcccgcg
     1681 gcgcgatacc gatcgccagc gagccccgca tcgaagtgcc cttcttcttc gttggcgtgg
     1741 tccacgaggg catctctccg gaagacgccg agcgcatgct cgtcatgccc atggagatcg
     1801 agcttcgtaa ggtagagggc gtcaaggagc tcaccgccta cgcatccgaa ggatctgcga
     1861 ccattctggt cgagttcgac gtcagccacg atctggacca ggcgctgctc gacgtccgcg
     1921 aagccgtgga ccgggcgcga ccggaacttc cgagcaccgc cgaagagccc tacgttcgcg
     1981 aggagaccac cgaggacttc cccatcgtgc aggtcaacct ggtcggcgag ggggtgcccg
     2041 agcgcatcct ctacaacctg gccatcgagc tgcgcgacga catcgaggcg atccca
//
LOCUS       DBNHLM010000336        12873 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1187778, whole genome shotgun sequence.
ACCESSION   DBNHLM010000336 DBNHLM010000000
VERSION     DBNHLM010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12873)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12873)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12873
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1187778"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="AC16QF_15530"
     CDS             <1..242
                     /locus_tag="AC16QF_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450021.1"
                     /translation="RFAELAANREAYFGAELTAIFDNNENLGRETAAWLLNHMAERQS
                     VRMYTRFVELAIDFRELADDAPNWLPPEEPCQITC"
     gene            complement(267..479)
                     /locus_tag="AC16QF_15535"
     CDS             complement(267..479)
                     /locus_tag="AC16QF_15535"
                     /inference="COORDINATES: protein motif:HMM:NF015957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR6450022.1"
                     /translation="MANSNLNNVRRRLRLDPRNGWIAGACAGFANRLQIDPAIVRVGV
                     VIAALFFPKTVIACYLLAWLVLDEAR"
     gene            complement(507..722)
                     /locus_tag="AC16QF_15540"
     CDS             complement(507..722)
                     /locus_tag="AC16QF_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450023.1"
                     /translation="MIIKDNAPAGPIARLSRITYPRASAANLMLLSVVIAAIGAIAYI
                     LHPDAAQPAPNTFHGAALLVSNWLLCG"
     gene            996..2609
                     /locus_tag="AC16QF_15545"
     CDS             996..2609
                     /locus_tag="AC16QF_15545"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6450024.1"
                     /translation="MDFHFASAWEAIADTVPDRTALICEGVTRTWADYDGRATRVAGL
                     LQAHGLGADTKVGLYLHNCNEYLEAQYGVFKLSGCPINVNYRYKAEELVYLLDNADAE
                     AVFYQGCYAPRIEEIRDQLPRVKLYVQVDDGTEDLLAGSVDYESSLDAAEPMARQKRD
                     PASVYMLYTGGTTGMPKGVMYPGGEFCYLLTAMGAGGRGLVPPQNLEALREYVAGIED
                     PPVTVVACPLMHGTGMWLGSLAPLLTGGTVITTPQLGLDPDLIWTLVGSHRATDLVVV
                     GDAFARPLLAALDEAERRGEPYDLSSLRQIVSSGVMWSREVKEGLLRHRDMMLVDAMG
                     STEGSMGSSVSSRASGPTTAKFQLAEGVKVFTDEGREVEPGSGEIGKLATSGFVPLGY
                     YKDPVKSAETFREVDGVRYSFPGDYATVEADGSITLLGRGSVCINTAGEKVFPEEVEE
                     AVKRHPAVVDCLVVGIPDERFGERVVAVASFHDDQDVSEPELIEFARDHLAGYKLPKQ
                     VLRVPQVQRAPNGKADYKWAKSTALAELT"
     gene            2619..3794
                     /locus_tag="AC16QF_15550"
     CDS             2619..3794
                     /locus_tag="AC16QF_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6450025.1"
                     /translation="MSGPLTGVRVLDLTSMVSGPVATMLLGDQGADVIKVEPLTGDLV
                     RYMGPNRDGLTSGFISSNRNKRSIAVDLKQEAGMDVVKRLAATSDVFVQNFRPGAIER
                     MGLGEDVVREIRPDIVYVSISGFGETGPYAHKRVYDPVIQALSGLAAIQRDRETGRPK
                     MVRTIIPDKTTALTAAQAITAALFARERSGEGQHVKLAMLDTMVAYLWPEGMAGFTFI
                     GKEVKAARAQFAQDLIFATTDGFITAGAVSDAEWQGMCRALEREEWIDDERFNTPNGR
                     IVNVQERLRLTAEVLETRSSAEWLARLDAEGVPCAPVLDRHEVFEHEQVRINEMVEEL
                     DHPVAGRIRQPRPAARFDRTPAGIHRHAPMLGEHTDELLQEMGLDADALRAAGVIGG"
     gene            3850..5427
                     /locus_tag="AC16QF_15555"
     CDS             3850..5427
                     /locus_tag="AC16QF_15555"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0032787 - monocarboxylic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6450026.1"
                     /translation="MTSSMMRLTQGIHRALSLYPRRRAVVSAEGEITWEQFADRVARL
                     AGALRELGVGPGDRVAMLALNSPRYVEYYFAVLWAGGVLAPVNCRWAAPEKAHCLNDS
                     GAKVLLADAAHLPEALELLETCGSVSHLVSCGEVEGSGTAALGYESLVSASLPVEDAE
                     RGGADLAALFYTGGTTGRAKGVMLSHDNFIANSMTALVNLGISEHSVHLHVAPLFHLA
                     GGSRLFTLTVAGGTHAVIPRFDAAEFLGAIERFGVTVTVIVPTMLNTLLQHPNFEAFD
                     LSSLDLLCYGASPMPESLLREAMERLPGARFLQSYGMTELSPVATMLEPQFHTFEGPL
                     AGKIRSAGRAVFNADVAIMSPDDLPLASGEVGEICVRGPMVMQGYWGQPELTAQALRG
                     GWMHTGDAGYLDDDGFLFLVDRVKDMIITGGENVYSAAVENVLYLHPGVHECAVIGVP
                     SDAWGEAVHAIIVPRGGARLDEAAVIEHCRAYLAGYECPKTVEFVDAELPKSGAGKIL
                     KAELRKTYWQGQARNIH"
     gene            5500..7146
                     /locus_tag="AC16QF_15560"
     CDS             5500..7146
                     /locus_tag="AC16QF_15560"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HIR6450027.1"
                     /translation="MQHTPLLMSNILDRGAKVAPDVEIVTATENGTRRQTYAEIRDRA
                     HQLAHALRDAGIGVGDRVATFMWNGSRHLEAYHAIGGMGAVLHTLNIRLSAKDLEYII
                     NHAEDRVIIADADVLPLLEPLKGRIPSVERIVVATEDGFEGWSTEVAEAVDYEEFIAG
                     KPTRFDWPRIDENSALGLCYTSGTTGNPKGVEYEHRSQYLHTLTQCMTDSMGLSATDC
                     VLGIVPMFHAMGWGMPWSCLMLGCKQVMPHRFMDTSRLLDLLVSEGVTMSAGVPTIWQ
                     GIKALVEADPERYDLSRLARMACGGSAPPPSLIRWYWDTLGVEMIQGWGMTETSPLAT
                     LSRRVMKRSHLELSEDDQFRNVAKAGQLMPGLELDIFDEDFNRLPHDGETVGEILIRG
                     PWICSEYYHDPQPEKFHDGWLITGDVGKIDSEEYLIISDRSKDLVKSGGEWISSVDLE
                     NHIVSLDGVEQACVVAQPHPKWDERPVALVVLGDGADVSLETVLAHCSQAFAKWQLPD
                     DVLYVDSIPLTSTGKMDKKIVRADLEAQGYVLPDLREKAG"
     gene            7207..7932
                     /locus_tag="AC16QF_15565"
     CDS             7207..7932
                     /locus_tag="AC16QF_15565"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6450028.1"
                     /translation="MTTRIRGVFFDLGGTLFSYRNMGRSTGPALLEAAARMGITGDPR
                     SIGKAYGLASREVNGAYAERDFYLHRDLFRDTFVRFVEQMEGQFDEAAYAWFRARQHE
                     LVLSGLELKPDCTETLSHLKSEGLYLSIVSNIDDDMLDPLVEREGLHDYLHHWTSSEA
                     AESCKPHRRFFEISLEKSGLAPGEVLFVGDSPEHDVQGAYEVGMNTVLIREEGLEPPL
                     QVGRQTVEPDYTINSLAEIRGLI"
     gene            7929..9107
                     /locus_tag="AC16QF_15570"
     CDS             7929..9107
                     /locus_tag="AC16QF_15570"
                     /inference="COORDINATES: protein motif:HMM:NF014954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6450029.1"
                     /translation="MSGRGEGPLPIPETPAALTPEWLTEALRRGGILDDQKVVDIEAE
                     VLGEGVGFLGDLLRLSLSYDRPGNLPDSLIAKLPKLDNRAMGEMLGAYERENCFYMEM
                     AGSLPLSTPRMYYGDFDRDRASEKQEDILRLFDRMPRFLLSFLPALGLRIAAAKKRRY
                     ILLLEDLSDAEPGDQVAGVSVRRCERALRAIARAHAACWGAELVDAFWLLPHDIDARM
                     RHSMFKGSRSTFSDFFGPELEARLEPYLRRLPDQGLDLIRRLNQAPTTLLHCDLRLDN
                     LFFREDEVIVIDWQLVRRGPAVYDVAYFLSSALDEAAGRAVVEGLLEAYLEELQAGGV
                     DGYTMDELLRDYRIALRVVLMTLSTVDQVELGDGRGIDLMRGWISRLHGRLAEMGEPG
                     "
     gene            9283..9873
                     /locus_tag="AC16QF_15575"
     CDS             9283..9873
                     /locus_tag="AC16QF_15575"
                     /inference="COORDINATES: protein motif:HMM:NF039725.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6316 family protein"
                     /protein_id="HIR6450030.1"
                     /translation="MPGKLPQWFRADEVYAYSGQWYFGSREGLHIGPYADREVAKAKG
                     TQVAKRLRAMDEAHQLKFVRKVLDREWDSVSKLIPTTGIGDAAAGSEPPPKEAVRHGE
                     GYRNWYRSGRYFQADGAWFFSTREGIDVGPFETEVQAKKHERRLVALLQRTQSAEEAF
                     QIIHEYKYRPSARSVGRVWANVTPASRTRPTKPRWA"
     gene            10005..10796
                     /locus_tag="AC16QF_15580"
     CDS             10005..10796
                     /locus_tag="AC16QF_15580"
                     /inference="COORDINATES: protein motif:HMM:NF013793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HIR6450031.1"
                     /translation="MYTCRQTELIVHVVCLLSLKGGSGKSTVVQSLSVCAQQHGHRTL
                     IVELDPQGTLKNWSKRRESEQPRVVQTLPQSVGDVLEEAKAEGVHWVFLDTPGHHVAS
                     ASAAAELADLILIPCKIQSVKDFDAVLPSLAEARRADKPAYVLMNQVPPNSPRLIRRR
                     QLQIQKHYDIAVLSRFLCRRADFEYCDERGLSAAELNPSGPAAEEIDRLYALLQSIFI
                     MRSLKVDKQALVPAPGTSVGPVSRSSEVEPRPLERGQARTEELTQ"
     gene            10821..11063
                     /locus_tag="AC16QF_15585"
     CDS             10821..11063
                     /locus_tag="AC16QF_15585"
                     /inference="COORDINATES: protein motif:HMM:NF042903.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HIR6450032.1"
                     /translation="MPQEKRKRRATAAPRTDARPPAMANRPPSRQGKKPVTAYVDKQV
                     HKQLRSLGLELEKSNQEMILEALNDYFERHRLSTKG"
     gene            complement(11483..12280)
                     /locus_tag="AC16QF_15590"
     CDS             complement(11483..12280)
                     /locus_tag="AC16QF_15590"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6450033.1"
                     /translation="MALVDYEIHGHIAVITLNRPEARNAINPEVAVRLADAWESVRDD
                     DQVRVAILTGTGSAFCAGADLGQLIPMVSGTRKPENEWDERVASEPGITNRALLRNFD
                     TEKPIIAAINGHAIAGGMEIVQGTDIRVSVAEAKFGVQEVKWAIFPAGGSTVRLPVQL
                     PYARAMELLLTGDLISAEEALNIGFLNYVTDDIMGKAMEIAEKIAANGPLAVKAIRRS
                     VKECLGKPEREALKIESEISGPVFRTEDAKEGPRAFMEKRAPEYHGR"
     gene            complement(12340..>12873)
                     /locus_tag="AC16QF_15595"
     CDS             complement(12340..>12873)
                     /locus_tag="AC16QF_15595"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6450034.1"
                     /translation="TFLSDLGFSLGEVAAVIAPAILIGSVLGASVTSWYLGRFGYKAT
                     VLTAAFAVVLAMLPIMWMGSLEEPTFVVVFAVTLNAIALPSFLDVSFQAARLKWASKS
                     QAATDYTSQIVTMAAGGGLAVAVGGFMAEYLGWFYYFLVAGTLVSGACFVLYYLFDTI
                     EDIVEERDRTEAEGGNF"
BASE COUNT         2272 a         4009 c         4278 g         2314 t
ORIGIN      
        1 atcgcttcgc ggagctggcc gctaatcgcg aggcctattt cggggccgag ctgaccgcca
       61 tcttcgacaa caacgagaac ctggggcgcg agaccgccgc ctggcttctc aaccacatgg
      121 ccgagcggca gtcggtgcgc atgtacacgc gtttcgtgga gctggctata gactttcgcg
      181 agctggccga cgacgcgcca aactggctgc cgccggagga gccctgccag atcacctgct
      241 gatggaaggg ctcaccgccg gtgggctcag cgagcttcgt ccagcaccag ccatgccagc
      301 aggtaacagg cgatcaccgt cttggggaag aacagggcgg cgatcaccac accgacccgc
      361 acgatggcag ggtcgatctg taaccgattg gcgaagccgg cgcaggcgcc ggcaatccag
      421 ccgttgcgcg ggtcgagacg cagtcgtctg cggacgttgt tcaagttcga atttgccact
      481 gggatctctc cggagtcttt ctgtcgtcag ccgcacagca gccagttgct gaccagcagg
      541 gccgcgccgt ggaaggtgtt cggcgccggc tgggcggcat cggggtgcag gatgtaggca
      601 atcgcgccga tggcggcgat caccacgctc agcagcatca ggtttgccgc cgaagcccgg
      661 ggataggtga tcctagacag gcgcgcgatc gggcctgcgg gtgcattgtc tttgattatc
      721 atctggtttt gccgtttcat tgtctgctcg cgttcatttg cagctgaagc cgggtcgttt
      781 ccgttggcct cgttactgaa tattcaaatg ccatgccaac tccgaacaaa cccgatctcc
      841 aggcttaatg ccgcctcgcc gggtggaagg ggcgacgggc gggcctgtgg tgttggcgat
      901 ttctgcgaac agttggcgaa ttcggtcggc ctggcgatgt caggacggac gacccattgc
      961 caatccggat ttcttctaca gggagggcgt ctgccatgga ttttcatttc gcatcggcct
     1021 gggaggccat cgccgacacg gttccggacc ggacggccct catctgcgag ggcgtgaccc
     1081 gcacctgggc ggattacgac ggccgtgcta cgcgggtggc cggtctgctt caggcccacg
     1141 gcctgggcgc ggatacgaag gtgggcctgt atctccacaa ctgcaacgag tatctcgaag
     1201 ctcagtacgg cgtcttcaag ctcagcggct gtccgatcaa cgtgaactac cgctacaagg
     1261 cggaggagct ggtttacctg ctggacaacg cggacgcgga ggcggtcttt taccaggggt
     1321 gttacgcccc gcgcatcgaa gagatccgcg atcagcttcc ccgcgtgaag ctctatgtgc
     1381 aggtggatga cgggaccgag gatctgctgg cggggtcggt tgactacgaa agctcgctcg
     1441 acgccgccga gcccatggcg aggcagaagc gggatccggc gagcgtgtac atgctctaca
     1501 cggggggcac cacgggcatg cccaagggcg tcatgtaccc cggcggcgag ttctgctacc
     1561 tgctcaccgc catgggcgcc ggggggcgcg gcctggtgcc cccgcagaac ctcgaggccc
     1621 tgcgggaata cgtggcgggt atagaggatc cgcccgttac ggtggtcgcc tgcccgctga
     1681 tgcacggcac gggcatgtgg ctgggcagcc tcgcgccgct gctcaccggc ggcacggtga
     1741 tcacgacgcc gcagctgggg ctggatcccg atctgatatg gaccctggtg ggcagccacc
     1801 gggcgacgga tctcgttgtt gtcggggacg ctttcgctcg tccgctgctg gccgcgctcg
     1861 acgaggccga gcgtcgcggc gagccttacg acctcagttc cctgcggcag atcgtctcca
     1921 gcggggtgat gtggagcaga gaggtcaagg aagggctgct gcgtcatcgg gacatgatgc
     1981 tcgtggacgc catgggctcg acggaaggaa gcatgggcag ctccgtcagc tcccgggcgt
     2041 cggggccgac cacggccaag tttcagctcg ccgaaggggt gaaagtcttc actgacgagg
     2101 gccgcgaggt tgagccgggt tcgggcgaga tcggtaagct cgccaccagc ggcttcgtgc
     2161 ccctcggcta ctacaaggat ccggtcaaat ctgcggaaac gttccgcgag gtggacggcg
     2221 ttcggtacag ctttcccggc gactacgcca ccgtggaagc ggacggcagc atcacgctgc
     2281 tcgggcgcgg cagcgtctgc atcaacacgg ccggcgagaa ggtgttcccc gaggaggtcg
     2341 aagaggccgt caagcgccat ccggccgtcg tcgattgcct ggtcgtcggc attcccgatg
     2401 agcgttttgg cgagcgcgtg gtggcggtcg cgtcctttca cgacgaccag gacgtatccg
     2461 agcccgaatt gatcgagttt gcccgcgacc accttgcagg ctacaagctt cccaagcagg
     2521 ttctgcgggt gccgcaagtg cagcgcgcgc ccaacggcaa ggcggactac aagtgggcga
     2581 aaagcacggc gctcgcggaa ctgacttgag aggttgatat gagcggaccg ctaactggag
     2641 tccgggtcct cgacctgacc agcatggttt ccggcccggt ggccaccatg ctgctcggtg
     2701 atcagggtgc cgacgtcatc aaggtcgagc cgttgacggg cgatctcgtc cgctacatgg
     2761 ggcccaaccg ggatggcctc acgtccgggt tcatcagctc gaaccgcaac aagcggagca
     2821 tcgcggtgga cctcaagcag gaggccggca tggacgtggt caagcgcctg gctgccactt
     2881 ccgacgtctt cgttcagaac ttccggcctg gggccatcga gcgcatgggt ctgggagagg
     2941 acgtggtgcg ggagatccgc ccggacatcg tctacgtgtc tatcagcgga ttcggagaga
     3001 cgggccctta cgcgcacaag cgggtgtacg acccggtgat tcaggcgctt tccggtcttg
     3061 ccgcgatcca acgcgaccgg gaaaccggaa ggccgaaaat ggtgcgcacc atcattccgg
     3121 acaagacgac ggcgcttacc gcggcccagg ccatcaccgc ggccctcttc gcccgggagc
     3181 gcagcggcga aggtcagcat gtgaagctag ccatgctcga caccatggtg gcctatctct
     3241 ggccggaggg catggcgggc ttcacgttca tcggcaagga agtgaaagct gcgcgggccc
     3301 agttcgctca ggacctcatc tttgccacca ccgacggctt catcacggca ggcgcggtgt
     3361 ccgatgcgga gtggcagggt atgtgccgcg cgctagagcg tgaggagtgg attgatgacg
     3421 agcggttcaa cacgcccaac ggccggatcg tcaacgtgca ggagcggctg cgcctgactg
     3481 ccgaggtgct ggagacgcgc agcagcgcgg agtggctggc ccgactcgat gccgaaggcg
     3541 ttccctgcgc ccccgtgctg gatcgccacg aggtcttcga gcacgagcag gtgaggatca
     3601 acgagatggt cgaagagctg gaccacccgg tggccggccg cattcgtcag ccgagaccgg
     3661 cggcgcggtt cgacaggacg ccggccggca tccaccggca cgcgccgatg ctcggcgagc
     3721 ataccgacga gctgctgcag gaaatgggtc tggacgcgga cgcgctcagg gccgccgggg
     3781 tgatcggcgg ctgatccgcc ggggctggct cgctcgtcgc ctgagctgcc tgtagaatcc
     3841 cgcgccgcca tgacttcctc aatgatgcgc ctcacccagg gcattcaccg ggccctaagc
     3901 ctctaccctc gccggcgggc ggtggtttcg gcggagggcg agatcacctg ggagcagttt
     3961 gccgatcgcg tggcgcgcct ggcaggggcg ctgagagagc tgggtgtggg tccgggcgat
     4021 cgagtcgcca tgcttgcgct caactcgccg cgctatgtgg agtactactt cgccgtgcta
     4081 tgggccgggg gcgttcttgc tccggtcaac tgccgctggg cggcgccaga gaaggcgcac
     4141 tgcctcaacg acagcggcgc gaaggttctg cttgcggacg cggcccacct cccggaagcg
     4201 ctcgagctgc tcgaaacctg cgggagcgtc tctcatttgg tctcctgcgg agaggtggag
     4261 ggctcgggaa ccgcggcgct gggctacgag tcgctggttt cggcgtcgct cccggtcgag
     4321 gacgccgagc ggggcggagc ggatctggca gcgctgttct acacgggcgg gaccaccggg
     4381 cgtgccaaag gcgtgatgct cagccacgac aacttcatcg ccaactcgat gacggctctg
     4441 gtgaatctgg gcatctcgga gcacagcgtc caccttcatg ttgcgccgct tttccatctg
     4501 gccggcggct cccggctgtt caccctgacc gtggccggag ggacccatgc ggtgatcccc
     4561 cgcttcgacg ccgccgagtt cctgggggcg atcgagcgct tcggggtgac cgtcaccgtc
     4621 atcgtcccga ccatgctcaa cacgctgctg cagcatccga acttcgaagc cttcgatctt
     4681 tcgtccctgg atcttctctg ctacggcgcc tcgcccatgc ccgagagcct tctgcgtgaa
     4741 gccatggagc ggctgccggg agcccgcttc ctgcagtcct acggcatgac cgagctgtct
     4801 cctgtggcta cgatgctcga gccgcagttc cataccttcg agggtccgct ggcggggaag
     4861 atccgttcgg ccggccgtgc cgttttcaac gcggacgtgg ccatcatgag ccccgacgac
     4921 cttccgcttg cgtcgggaga ggtgggagag atctgtgttc gcggacccat ggtcatgcag
     4981 ggatactggg gccagccgga gctcaccgct caggcgctgc gcggtggctg gatgcacacg
     5041 ggagacgccg gttacctcga cgacgatggt tttctgtttc tcgtcgatcg ggtgaaggac
     5101 atgatcatca cgggcggcga gaacgtctac tctgcggccg tggagaacgt gctgtacctg
     5161 caccccggag tgcacgagtg cgcggtgatc ggggtcccga gcgatgcctg gggcgaggcc
     5221 gttcacgcca tcatcgtgcc tcgcggtggc gcccggctcg acgaggcggc cgtcatcgag
     5281 cactgccgtg cctaccttgc cggctacgag tgcccgaaga cggtggagtt cgtcgacgcc
     5341 gagctgccga agagcggggc gggaaagatc ctgaaggccg agcttcgcaa gacctactgg
     5401 cagggtcagg cgcgcaacat tcactgagga tgatagactc gctgccctca cgctgaattc
     5461 accggaacga aagtctaagg gaggtggcga accgtggcga tgcagcatac gccattgctg
     5521 atgtccaata ttctggatcg tggcgcgaag gtggcgcccg acgtggagat cgtcaccgct
     5581 accgaaaacg gcacgcggcg gcagacctat gccgagatcc gcgatcgtgc ccaccagctc
     5641 gcccacgccc tgcgtgatgc cggcatcggc gtgggagatc gcgtggcgac tttcatgtgg
     5701 aacgggtccc gtcatctgga ggcgtatcac gccatcggcg gcatgggcgc ggtgctgcat
     5761 acgctgaaca tccgcctttc ggccaaggac ctcgagtaca tcatcaacca cgccgaggat
     5821 cgcgtgatca ttgcggatgc ggacgtgttg ccgctgctgg agccgctcaa gggacggatt
     5881 cccagcgtgg agcggatcgt ggtcgccacc gaggatggtt tcgaaggctg gagcaccgag
     5941 gtggccgagg ccgtcgacta cgaggaattc attgccggca agccgacccg tttcgactgg
     6001 ccccggatcg acgagaactc cgccctcggc ctgtgctaca ccagcggcac cacgggcaac
     6061 ccgaagggcg tcgagtacga gcaccgttct cagtatctgc acacgctcac tcagtgcatg
     6121 accgactcca tgggcctctc cgccacggat tgcgtgctcg gcatcgtgcc catgtttcac
     6181 gccatgggct ggggcatgcc ctggtcgtgc ctcatgctgg gctgcaagca ggtcatgccg
     6241 caccggttca tggatacgtc gaggctgctg gatctgctgg tgtccgaggg cgtgaccatg
     6301 tcagccggcg tgccgaccat ctggcagggc atcaaagcgc tggtcgaggc cgacccggag
     6361 cgctacgacc tttcccgcct ggctcgcatg gcctgcggcg gctccgcgcc gccgccgtcg
     6421 ctgatccgct ggtactggga caccctcggg gtagagatga ttcagggatg gggcatgacc
     6481 gagacgtcgc cgctggccac gctgtcgcgg cgggtgatga agcgttccca cctcgagctc
     6541 agcgaggatg atcagttccg caacgtcgcc aaagccggac agctgatgcc cggtctggag
     6601 ctcgacatct tcgacgagga tttcaaccgc ctgccccacg atggcgaaac cgtcggcgag
     6661 atcctcatcc gtggaccctg gatctgctcg gagtactacc acgatcccca gccggagaag
     6721 tttcatgacg gctggctgat cacgggcgac gtcggcaaga tcgacagcga ggagtacctg
     6781 atcatcagcg atcgctccaa ggatctcgtc aagagcggcg gggagtggat ctcctccgtg
     6841 gacctggaga accacatcgt ctctctcgac ggggtcgagc aggcctgcgt ggtggcgcag
     6901 ccgcacccga aatgggatga gcgtccggta gcgctcgtgg tgctcggcga cggagccgac
     6961 gtcagcctgg agaccgtgct ggctcactgc agccaggcgt ttgcaaagtg gcagctcccc
     7021 gatgacgtgc tttacgttga cagcattccg ctgacgtcca ccggcaagat ggacaagaaa
     7081 atcgtccgcg cggacctgga ggctcagggt tacgtgctcc ccgatctgcg ggagaaagcg
     7141 gggtgatcgg ttggaaattc cggcgttttg gccgatggcc gcgcatcgcg tgagcatcgt
     7201 ttcgccatga cgacgcgcat caggggcgtg ttcttcgacc tgggcggcac gctgttcagc
     7261 tatcgcaaca tgggccgctc caccggcccc gcgctgctcg aggcggctgc gcgcatgggc
     7321 attactggcg atccgcgcag catcggcaag gcgtacgggc ttgcatcccg ggaggtcaac
     7381 ggcgcttacg ccgagcggga cttctacctg caccgggatc tcttccgcga caccttcgtg
     7441 cgctttgtgg agcagatgga ggggcagttc gacgaggccg cctacgcctg gttccgcgcc
     7501 cggcagcacg agctggtgct gagcgggctc gagctcaagc cggactgcac cgaaaccctt
     7561 tcgcatctga agtcggaagg gctgtacctc tccatcgtct ccaacatcga cgacgacatg
     7621 ctcgacccgc tcgtcgagcg ggaagggctt cacgactatc tgcatcactg gacgagctca
     7681 gaggctgcgg agtcgtgcaa gccccaccgg cgattcttcg agatcagcct ggagaagagc
     7741 ggcctcgcgc ctggcgaggt tctgttcgtc ggcgattccc ccgagcacga cgtccaaggc
     7801 gcctacgagg tgggcatgaa cacggttctc attcgtgagg agggtctcga gcctccgctt
     7861 caggtgggca ggcagacggt ggagccggat tacaccatca acagccttgc ggagatcagg
     7921 gggctgatat gagcgggcgc ggagagggcc cgctgccgat tccggaaacc ccagccgcgc
     7981 tgacgcccga gtggctgacg gaggcccttc gccggggcgg gattctggac gatcagaagg
     8041 tcgttgatat tgaggccgag gtgcttggcg agggcgttgg cttcctgggt gacctgctga
     8101 ggttgtccct cagctacgat cgccccggga accttcctga ctcgctgatc gccaagctgc
     8161 cgaagctgga taaccgcgcc atgggggaga tgctcggtgc gtacgagcgc gaaaactgct
     8221 tttacatgga aatggcgggg agcctgccgc tgagcacccc tcgcatgtat tacggcgatt
     8281 tcgaccgcga cagggcatcc gagaagcagg aagacattct ccgcctcttc gaccgcatgc
     8341 cgcggtttct cctctcgttc cttcccgcgc tggggctgcg gatcgccgcg gccaagaagc
     8401 ggcgctacat tctgctgctc gaagatctca gcgatgccga gccgggcgat caggtggcgg
     8461 gcgtcagcgt gcgacgctgc gaacgggctc tgcgcgccat tgcccgcgcc cacgccgcgt
     8521 gctggggagc ggagctcgtc gacgcgttct ggctgctccc ccacgacatc gatgcgcgca
     8581 tgcggcacag catgttcaag ggctcgcgat cgacattctc ggatttcttc ggccccgagc
     8641 tggaggcgcg cctggaaccc tatctgcgac gccttcccga ccagggcctg gacctgatcc
     8701 gccgcttgaa ccaggcaccg accacgctgc ttcactgtga cctgcgactg gacaatctgt
     8761 tcttccgtga ggacgaggtg atcgtcatcg actggcagct cgtccggcgg ggcccggcgg
     8821 tgtacgacgt ggcctacttt ctctccagcg ccctggacga ggcggccggc cgcgcggtcg
     8881 tggagggctt gctcgaggct tacctggaag agctccaggc gggcggcgtg gacgggtaca
     8941 cgatggacga gctgctgcgc gactatcgca tcgccctgcg cgtcgtgctg atgacgcttt
     9001 cgacggtgga ccaggtggaa cttggcgacg gtcgtggcat cgacctgatg cgtggctgga
     9061 tctcccgcct gcacggccgg ctcgcagaaa tgggagagcc ggggtgaatc aggtttgcgc
     9121 ggccgtcgtc gtgagggccc gcgcacggcg acggggcctg ttgcccctct ccgagccggc
     9181 cggaagtttg cgctgcgtca cacactgaac gcgacgcagc tcgcccggcg aacgaggtcg
     9241 cgacgttaga atcgggtgat cgagtccacg ttggagccca gcgtgcccgg aaaactgccg
     9301 cagtggttca gagccgatga ggtttatgcc tacagcggtc agtggtattt cggctcgcgt
     9361 gaggggcttc acatcgggcc ctatgcggat cgggaagtcg ccaaggcgaa aggcacccag
     9421 gtagccaagc gcctgcgcgc gatggatgag gcgcaccagc tcaagttcgt ccgcaaggtc
     9481 ctggataggg aatgggacag cgtctccaag ctgattccaa ccacgggcat cggcgatgca
     9541 gccgcgggct ccgaacctcc tcccaaggaa gccgtccgcc acggcgaagg ctatcgaaac
     9601 tggtatcggt ccggccgcta tttccaggcc gacggcgcgt ggttcttctc tacccgcgag
     9661 ggtatcgacg tcggtccctt cgaaacggaa gttcaggcaa agaagcatga acgccggctg
     9721 gttgcgcttc tgcagagaac ccaatccgcg gaagaggcgt ttcagatcat tcacgaatac
     9781 aagtatcgtc cctcggcgcg gtcggtgggc agggtctggg cgaacgtcac ccccgcgagt
     9841 cgaaccaggc cgacgaagcc gcgctgggca tagatcccgg cccggtcttc ccgctcttta
     9901 actccgtttc ttttacgcat ggttgccgtg tcgccagcgc gataagtact tttaccttac
     9961 aaaaacgtgg gttaacaagc ggctggaacg tgtgttacgg tgacatgtat acatgtcgac
    10021 aaacggagtt gattgtgcat gttgtctgcc tgctgagcct caaaggagga tcagggaagt
    10081 ccacggtggt gcagtcgctg tcggtttgcg cgcagcagca cgggcaccgc accctgatcg
    10141 tcgagctgga tccccaggga actctcaaga actggtccaa acgccgcgag tccgaacagc
    10201 cccgcgtcgt tcagacgttg ccgcagtcgg tgggcgacgt tctggaggag gctaaagcgg
    10261 aaggtgtgca ctgggtgttc ctggacacgc ccggtcacca tgtcgcgtct gcatccgccg
    10321 cagcggagct tgcagatctg attctgattc cctgcaagat acagtcggtc aaggacttcg
    10381 atgccgtgct gccgagcctc gccgaagcgc gccgggctga caagccggcc tacgtcctga
    10441 tgaatcaggt gccgcccaac tcgccgcggc tgatccgtcg tcgccagctg cagatacaga
    10501 aacactacga cattgccgtg ttgtcgcgat tcctgtgccg gagagccgac ttcgaatact
    10561 gcgacgagcg cggactgtcg gctgcggagc tcaacccctc cggcccggct gcggaagaga
    10621 ttgaccggtt gtacgcgctg ctgcagtcga tcttcatcat gcgcagcttg aaggtggaca
    10681 agcaggcgct ggttcccgca ccaggcacat cggttgggcc ggtcagccgc tcgagcgagg
    10741 tagagccccg gccgctggag cgcgggcagg caagaaccga agaactcacc cagtaaacaa
    10801 caacacacag ggaaggacac atgccgcagg agaagcgcaa gcggcgggcc acggctgctc
    10861 cgcgcacgga cgcccgccca ccggccatgg ccaaccggcc gccgagcagg cagggcaaga
    10921 agcccgtgac cgcctacgtc gacaagcagg tacacaagca gttgaggtcg ctgggtctgg
    10981 agcttgagaa gtccaatcag gagatgatcc tggaagcctt gaacgactac ttcgaacggc
    11041 accggctgag cactaaaggc tgaggcatca cagcgtccac gagttccgct gacggggtcg
    11101 ccgccagcgg gaacgttccg ttacgttggc attacgatgc gaatacgatc tgcacacggg
    11161 attcgaaatc gtgaaggcct cgagagagta tgctgtgtct cctcagtaag gaggtgtcgc
    11221 ttggctttta tgctccggtt tccagccttg acctgctcat cggacggccc tgtcttcgag
    11281 aggctgaccg atgcgattcg ggcaggaagg cgcataggtt ccagattcgc cgtctggcag
    11341 gacgacgaga tgcgagcagt ctgggcgcct ccggcagctg ctcagatgcg caaaagccga
    11401 gagcgcaggc tggcgggcgg cagcagtgag cttcgccgcg cgccgagacc cgtcggggag
    11461 cgcttcgctt agcccgtcgt tgtcagcgtc cgtggtattc gggcgcccgc ttctccatga
    11521 aagctcgagg cccttccttg gcatcctcgg tacggaacac gggtccggat atctccgatt
    11581 cgatctttag agcctcgcgc tcgggcttgc ccaagcactc cttcacggaa cgtcggatcg
    11641 ccttcactgc cagtggtccg ttcgccgcga tcttctcggc gatttccatc gccttgccca
    11701 tgatgtcgtc ggtaacgtag ttgaggaacc cgatgttcag ggcttcctcg gcagagatca
    11761 gatcaccggt cagcagcagc tccatagcgc gcgcgtaggg cagctgaacc ggcagccgca
    11821 ccgtggatcc gccggcgggg aatatggccc acttgacctc ctgaacgccg aacttcgcct
    11881 ccgccacgga gacgcggatg tccgtgccct ggacgatctc catgccacct gcgatggcgt
    11941 ggccgttgat tgccgcaatg atcggcttct cggtatcgaa gttgcgcagc agcgcccggt
    12001 tggtgatgcc cggctcggaa gccacacgct cgtcccactc gttctcgggc ttgcgcgttc
    12061 cggagaccat tgggatgagc tggcccagat cggccccggc gcagaacgcg ctgcccgtgc
    12121 ccgtcaggat ggcgacgcgg acctggtcgt cgtcacgtac cgattcccag gcatctgcca
    12181 ggcgtacggc cacctccgga ttgatcgcgt tgcgggcttc cggccggttc aaagtgataa
    12241 ccgcgatgtg tccgtgaatt tcgtagtcta cgagtgccat tggatcctcc ccttcctctg
    12301 catttgttgt gacgcgggct tggctctgct cgtgaagggt cagaagttac cgccctcggc
    12361 ttccgtccgg tcgcgctcct cgacgatgtc ttcgatcgtg tcgaagagat agtagagcac
    12421 gaagcaggcg ccgctcacca gggttcccgc gaccaggaag tagtagaacc atcccaggta
    12481 ctcggccatg aaaccgccca cggcgaccgc cagccccccg cccgccgcca tggtgacgat
    12541 ctgcgaggtg tagtcggtgg ccgcttgaga tttggacgcc cacttcaggc gcgcagcctg
    12601 aaagctcaca tcgaggaacg aaggcagagc aatggcgttg agggtcaccg cgaagaccac
    12661 gacgaatgtc ggttcctcca gcgatcccat ccacatgatg ggcagcatcg ccagaaccac
    12721 cgcgaacgcc gccgtgagaa ccgttgcctt gtacccgaat cgtcccagat accagctggt
    12781 gaccgacgcc cccagaaccg aaccgatcag gatggccggg gcgatcacgg cggcaacctc
    12841 gccgaggctg aagcccagat cggaaagaaa cgt
//
LOCUS       DBNHLM010000337         3590 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_529913, whole genome shotgun sequence.
ACCESSION   DBNHLM010000337 DBNHLM010000000
VERSION     DBNHLM010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_529913"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..727
                     /locus_tag="AC16QF_15600"
     CDS             320..727
                     /locus_tag="AC16QF_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450035.1"
                     /translation="MLKVGRERSARQITALLLVTASIIGPAARAEPWAFTGRVGTAQG
                     DQAYFGSTGLGLSAFTGNVRLPNGRFIERDRFGLDAQWYRGPFGVMTEVSVGDDDGAD
                     VVNVLTELNLISRRERFAAYVQERWFNKKPNES"
     gene            1535..2020
                     /locus_tag="AC16QF_15605"
     CDS             1535..2020
                     /locus_tag="AC16QF_15605"
                     /inference="COORDINATES: protein motif:HMM:NF016955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HIR6450036.1"
                     /translation="MSLREKIAGDGYVRLRNRPFLEASMAASALIAMADEHVNAAERL
                     SIESVMENIRRLNVFEPRTAIRLHCQFVAAIRANAATGEALALRTISRFKGDLKAGEM
                     LVNIAHYTAHADNELVPEELVAIRRVYRELGIDPAHHEPAIEYEEPDGPRSRTYRLRT
                     A"
     gene            complement(2374..2583)
                     /locus_tag="AC16QF_15610"
     CDS             complement(2374..2583)
                     /locus_tag="AC16QF_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450037.1"
                     /translation="MKQGHARNKLLQTARSIFYIEVSPENQVAVTDLFRSCDYDIFHL
                     QGDASEQRVQECTFYTVARPRDAAG"
     gene            complement(2934..3009)
                     /locus_tag="AC16QF_15615"
     tRNA            complement(2934..3009)
                     /locus_tag="AC16QF_15615"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2974..2976),aa:Gly,seq:gcc)
     gene            complement(3080..>3590)
                     /locus_tag="AC16QF_15620"
     CDS             complement(3080..>3590)
                     /locus_tag="AC16QF_15620"
                     /inference="COORDINATES: protein motif:HMM:NF014585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR6450038.1"
                     /translation="GKDGLSTTAFPSGVRTTSTEVNEATAPLLIWRKEYRQDSAGPGE
                     HRGGLGQTVEVAHARGESFVISKMFDRIRHPARGRNGGGDGEPGRVYVKGGDDFRGKG
                     RDVVPPGATLVMETPGGAGMGRVAERDPDRIAADVEAGLVSPDAARDRYGLDAGVRQN
                     DDEGGAADV"
BASE COUNT          674 a         1169 c         1111 g          636 t
ORIGIN      
        1 gcgacgatct cgctgtccac cagcacccca tcgcagtcga agatgagcgc cgccgggtac
       61 tcagggacca aggggaacgc tcctgtggat cacctggcac gaaccgtgta gaccatccgg
      121 cggcggcgtc attgatcgct gcctgagtct gtttggcttg agattcgcat tttgcgacgc
      181 atttcgcgaa ttctgttgca ttgcgcggcg ctcgatggcg ctgagagccc tgattatccg
      241 gcgatccgtc agctggcgca ccaattgcat tcattgccat agaagttgcg ctcgacggtt
      301 gcggcggacg gagacaacca tgctaaaggt cggacgggaa cgctcggctc gccagatcac
      361 ggcgctcctg ctggtcacgg catcgatcat cgggcccgca gcccgggcag agccctgggc
      421 attcaccggc agggtcggca ccgcgcaggg cgaccaggcg tacttcggct ccacagggtt
      481 gggcctgtcg gcgttcaccg gcaatgtccg cctgccgaat ggtcgtttca tcgagcgcga
      541 ccgcttcggg ctggatgcgc aatggtaccg ggggccgttc ggcgtgatga ccgaagtctc
      601 ggtcggcgat gacgacggtg ccgacgtcgt gaacgtgctc accgaattga acctgatctc
      661 gaggcgcgag cgtttcgccg cctatgttca ggaacgctgg ttcaacaaga agcccaacga
      721 aagctgaaaa gatcagacct cctgggcggc aggtgcccgc tacctggctt cgcgcaatct
      781 cgaggtcagc ttccagtacc tcaacgagca ggaggtctac ggaccccgcg acgaggagtc
      841 cgttgggcag cttcaattca ggtacaggac acttcaatga cgctttcccg caaggacggt
      901 cctctggcaa gtctcacact tctggccgcg ctgctcgcag ccggcggacc ggcgtatgca
      961 ccgttggcaa atccgcgcgg gatgccctgg agcgcggtct gccctttccc ttctaattcg
     1021 acgagttcgg tcgccactcc ctgtacgtgg tcaccgccgg cggcaaaccc aaaggattgg
     1081 tccatgcgcg aaccgagctg ggtcagtggg gactcgacga gatcgtccgg gcgttggacc
     1141 acgacctgcg ggttcacgat ttccggtttc agcggacgcg agaccccgct cgcaggctca
     1201 tcaaaggcga cgagtttcgc gccctgctca caggtaagag cacccgagaa ttgatgacgc
     1261 tgctcagcga cggtgagtta cgcgcggcgg tcggccgatg gatcaaactg cagccatcgc
     1321 aatccaacgt cgtggcgctt cccgtaggga aaacttgaac acacttcacg cttgccgtaa
     1381 tacgcggtcg gggttttgat ccattgccat gggatgtgcc cgcctcttgt gaactgattc
     1441 gtggcaggtg catcggcccc atggttcgat cgctctgggc gtagaacaga gcattcaatg
     1501 ccggcgccca cgttcgaggg aagcggagtc gatagtgagt ttgagggaga agatcgccgg
     1561 agacggttac gtacgattga gaaatcgtcc gttcctcgag gcttccatgg cagccagcgc
     1621 gctgatcgcc atggccgacg agcacgtgaa cgccgcggag cgcctgtcca tcgagagcgt
     1681 gatggagaac atccgacggc tcaacgtctt cgaaccacgc accgcgattc ggcttcactg
     1741 tcagttcgta gctgcgatca gggccaacgc tgctacgggc gaagcgctgg cgctgcgcac
     1801 catctcccgc ttcaagggcg acctgaaggc cggcgagatg ctggtgaaca tcgcccacta
     1861 cacggcacac gccgacaacg agctcgtgcc ggaggaactg gtcgccattc gtcgcgtcta
     1921 cagggaactc ggcatcgatc cggctcacca cgaaccagcc atcgagtacg aggaacccga
     1981 cggcccgcgt tcgcgcacat accgcctgcg caccgcctga gtcaggaatc cagcgcctgc
     2041 cgatacggcg tcatgttcat tccgaatacg ccgtagcggg cctcaacggc atcgatggtc
     2101 gccgtcgtcc agtaggggga accgggctgc gggaaaccca gaacggcggt cggtccccgg
     2161 cgccgtactt aggccagacg tcagattggg tcagcctcag ttcggtcacc gcgacgccga
     2221 gaagctcggc aaccgcgcgg atcagccgta ggtgcagccg cgggtgcagc cgcgggtgca
     2281 gccgatctgc gcgggctcgg cgagcagcca tcgatccgag gcgatgccgt gatctcccgt
     2341 cacggcgctg ccgaagcgcc cttgccggac gggttacccg gcagcatcgc ggggccgggc
     2401 caccgtgtag aaggtgcact cctgcacccg ctgctcggag gcgtcaccct gaaggtggaa
     2461 gatgtcgtag tcacagcttc ggaacagatc ggtgactgcc acctggtttt ccggagacac
     2521 ctcgatatag aagatcgacc gcgcggtctg gagcagcttg ttccgtgcgt gcccctgttt
     2581 cacaacagcg aactccagca cccccgcgtg atcggcgatg gcggcgcaca gaacgttgac
     2641 cggctggtag gccgatgaca ggcggcggct cgagcggatt atccgatctg cgtaagccgg
     2701 atcggcttcc agcgcgtaaa tggcgccctg cggaccaacc ctgtacgcgg atgtcggacc
     2761 gcgccgtgac gtacataagc tcgcccccga actccctggg caaacgttta ctgaagtcga
     2821 aattgacgag gttgcggaaa ttcatggttc ctcgactgtc ccgggaaacc gccctctcat
     2881 caacatcatc ggcgtgcatg ggagaaagaa tcgacgaacg cgtcgaaatg aagtggagcg
     2941 ggaaacgaga ctcgaactcg cgaccccaac cttggcaagg ttgtgctcta ccaactgagc
     3001 tattcccgcc ctggaagaat ccgtccggtc cgacggccgg acgcgaacta tagccgaatc
     3061 ggcccgccga gtggaagcgc tagacgtctg ccgcgccgcc ttcatcgtca ttctgccgga
     3121 ccccggcgtc gagcccgtag cgatcgcgcg ctgcatcggg gctgaccaga ccggcctcga
     3181 catcggcggc gatccggtca gggtcgcgct cggccaccct gcccatgccg gccccgcccg
     3241 gggtctccat gaccagcgtg gcgcctggtg gcaccacgtc cctgcccttg ccccggaagt
     3301 catctccccc tttcacgtag acccgaccag gctcgccgtc tcccccgcca tttcgacccc
     3361 gggcaggatg ccggatgcga tcgaacatct tggagatcac gaaagattct ccccgcgcat
     3421 gcgcaacctc caccgtctgc cccagcccgc cgcgatgctc ccccggacca gccgagtcct
     3481 gccgatactc cttgcgccat atcagcagcg gcgccgttgc ttcgttgacc tccgtcgacg
     3541 tggtgcgcac ccccgaaggg aaagccgtgg tggaaagacc atccttgccc
//
LOCUS       DBNHLM010000338         9376 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_50800, whole genome shotgun sequence.
ACCESSION   DBNHLM010000338 DBNHLM010000000
VERSION     DBNHLM010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9376)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9376)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9376
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_50800"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="AC16QF_15625"
     CDS             <1..766
                     /locus_tag="AC16QF_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015371271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR6450039.1"
                     /translation="TVVEATSGNTGIALAMVCAATGHPFVATMVETFSIERRKIMRAL
                     GAKVILTPAAERGSGMVRRAEELAAEHGWFLARQFENPANPAYHRNTTGPEILKDFAG
                     RRLDYWVTGWGTGGTLTGAGEVIKVARPDVKIIAAEPEQASMLAGKEWSPHMIQGWTP
                     DFIPAVLNPEVCDAIVPVNQDVAKDTALALARNEGIFVGVSAGGTLAAALDVAEDAAP
                     GSVFVVMLPDTGERYLSTFMFEGINEGSDDDWLESL"
     gene            836..2044
                     /locus_tag="AC16QF_15630"
     CDS             836..2044
                     /locus_tag="AC16QF_15630"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6450040.1"
                     /translation="MAELSLESIFTPEIIADPYPLYRQLRETSPVLELPDANMVILTR
                     YADVQTVLRDRRLGHADESMLTRGDLEQARNNPAIANLRRTMLLKNPPDHTRLRGLVV
                     KAFDARRVEAMRTRIRAIANELVDAFVDAGSGDLVRLFTHPLPVIVICDLLGVPKADQ
                     AEFVQGTRISGRLIDPSPMTEEELAQANASTTESQRYFEQLCDERRQAPSDDLITALV
                     QSETEHGKLSKEELTSNISLLFAAGHETTVNLMGNGLIALYRNRDQLDRLRADPSLMP
                     NAVEEFLRYDSSVQLTGRDALEDAEIAGIALPRGRAVLALLAAANRDPEAFDRPEELD
                     IARKRIKPVSFGGGIHLCLGAQLARIEAHEALNVLLERLPNLELDDVATPDWKQTITL
                     RGVRTLPATW"
     gene            complement(2069..2497)
                     /locus_tag="AC16QF_15635"
     CDS             complement(2069..2497)
                     /locus_tag="AC16QF_15635"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6450041.1"
                     /translation="MIELDEEIKGRLDRAIEEHKTVTVAYVDVDGKPHISFYGSTHVH
                     SSNQLAIWVRNPQNDLIRTIPDRPNVAIIYGDIGGRVYYTFEGTASVTEDAEEQERVY
                     QEMHPVERQFDPDRGGVAVVVDLDRVTLLSAAAGKQVMER"
     gene            2695..3051
                     /locus_tag="AC16QF_15640"
     CDS             2695..3051
                     /locus_tag="AC16QF_15640"
                     /inference="COORDINATES: protein motif:HMM:NF023178.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HIR6450042.1"
                     /translation="MPEILFLLVAGHAICDFVLQSEAMATGKNHRLKEKMRERHGPSF
                     PSWPYWLTSHTITHGAAVFLITGNLLLGMIETVLHTVIDYTKCAGRISLLTDQILHGA
                     CKIGYVYYLWHAGALG"
     gene            complement(3072..3200)
                     /locus_tag="AC16QF_15645"
     CDS             complement(3072..3200)
                     /locus_tag="AC16QF_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450043.1"
                     /translation="MSGQDQGGFRANETIARLVAFVGPYILWGTLGLVVWGMLLTA"
     gene            complement(3726..4547)
                     /gene="cysQ"
                     /locus_tag="AC16QF_15650"
     CDS             complement(3726..4547)
                     /gene="cysQ"
                     /locus_tag="AC16QF_15650"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HIR6450044.1"
                     /translation="MDAELTNALIDLTRRAGDAILEIYQTDFDVEHKGDSSPLTQADL
                     ASHAVIAAGLAELMPDVPVLSEESSPPEFAVRQGWSRYWLVDPLDGTKEFVSRNGEFT
                     VNIALIEGHRAVFGVVGVPTQGYVYTGDLATATALRHGPEGAVPLRGRKRNDSDPLVV
                     VASRSHGGQRLEDYLDALARSIGEIERKPVGSSLKLCILAEGHADLYPRLGPTSEWDI
                     AAAHAVLEAAGGAVWAVGGGALSYNAKESFLNPEFFAAADGSYPWRTVLPAVPAA"
     gene            complement(4557..5114)
                     /gene="nudE"
                     /locus_tag="AC16QF_15655"
     CDS             complement(4557..5114)
                     /gene="nudE"
                     /locus_tag="AC16QF_15655"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008736.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HIR6450045.1"
                     /translation="MPVPEIKAVRTVASTRFFRIEEIDLEFATGERRTYERLPGVGTP
                     AVIVVPVNERGELLLIREYCAGFHEMQLTMVKGAANRDEALEDAANRELKEEIGMGAR
                     QVRFLKCLSVAPGHMGFTINVMLATDLYPEQLPADEPEPPELVPWPLARLDELIAGDE
                     FREARAIAALSVARPWLDGRLDNEE"
     gene            5195..5851
                     /gene="yrfG"
                     /locus_tag="AC16QF_15660"
     CDS             5195..5851
                     /gene="yrfG"
                     /locus_tag="AC16QF_15660"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF011564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HIR6450046.1"
                     /translation="MICWDSVDTVLLDMDGTLLDLNYDNTLWNQLVPMRYSEAKSIPV
                     EAARELLFARMLDTRGQLDFYCLDHWAEFTGLDIVGLHRELTDLIRYRPYAQEFLVWL
                     RARGKRSLLVTNAHRESLKVKDAHSDVTSKLDADVSCHDYGEPKESPAFWAELMARHP
                     YEPARTLLIDDNDAVLTAAREFGIGHLLTVAQPDSGRPPREGLGYPAFTDFREILPDE
                     "
     gene            5844..6266
                     /locus_tag="AC16QF_15665"
     CDS             5844..6266
                     /locus_tag="AC16QF_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR6450047.1"
                     /translation="MSEGQAARLDRWLWAARFFKTRAQAKAAIEGGKVHLHLNRDESG
                     ATVRPKVSREVSVGDVVTVRRGGAPETVVIRAIAHQRGSASVAATLYDETPASIEGRE
                     AQRAQRQLERLGLKVPSTKPGKRDRRALKKLKEDSAPS"
     gene            6263..7159
                     /gene="hslO"
                     /locus_tag="AC16QF_15670"
     CDS             6263..7159
                     /gene="hslO"
                     /locus_tag="AC16QF_15670"
                     /inference="COORDINATES: protein motif:HMM:NF013589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIR6450048.1"
                     /translation="MTADRLHRFSFQNAPIRGNWVRLDAVLAEVFARQSYPGRLQAVL
                     AEALSAVAMLADTVKFQGTISLQSRGRGPVTTLLAECRSRSVLRGIARWDRSAGIPED
                     APLGELLGDARLAISLVDSDPATPRPVSYQGLVTLDHESLAGNLEGYFAFSEQLPTRF
                     YFGRAGHTVTGLLLQRLPADDDATEIDLDQHESDWKQIQEAAGESSADLAALPPQALL
                     RRLFPSRPLTLQPGRPLSFGCTCSRDRSTASLNAVPAPELLDILHSDGEVTVTCEICG
                     TVYAYDPIDIHLLLQREAPQLH"
     gene            7253..8818
                     /locus_tag="AC16QF_15675"
     CDS             7253..8818
                     /locus_tag="AC16QF_15675"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HIR6450049.1"
                     /translation="MAVDIESTAKHTTHLNLAAAALAELAVLRGEGQFASNGAIVVRT
                     GHRTGRSPRDRFIVEEPSTADAIDWGTINQPIAQDVFDALWDRVQIHLEDRERFVSNL
                     HVGADPEHYLPIEVTTEYAWHALFGRALFIKPENYNPHNKSVWNIANAPSFVCDPERD
                     GTASDGTVMINFAQRKVLLAGMRYAGEMKKSMFSVLNFLLPEKDVLPMHCSANMGHDG
                     AVTLFFGLSGTGKTTLSADPDRLLIGDDEHGWGKGTVFNFEGGCYAKCIDLTRENEPL
                     IYDAIRFGAIVENVIIDQDTREPDYTDGSLTENTRACYPRSNIEAKVTDNRGGEPGQV
                     VFLTCDVSGVLPPVSLLSKEAAAYHFLSGYTAQVGSTEVGSTEAYKATFSTCFGAPFF
                     PRAAGVYADLLIKRIEEFGSKVFLVNTGWTGGGYGVGKRFAIPDTRAIIRAIQSGALD
                     DAETRRLPGFNLDIPTSVPGVDAGLLNPRDTWSDKAAYDESARSLIAKFAENFEKFSV
                     AQAIVDAGPTTDY"
     gene            8858..>9376
                     /locus_tag="AC16QF_15680"
     CDS             8858..>9376
                     /locus_tag="AC16QF_15680"
                     /inference="COORDINATES: protein motif:HMM:NF016174.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HIR6450050.1"
                     /translation="MAHAAAPIDPALLDALRDQPASLREIQRGIEKESLRVTPEGSLS
                     DRPHPAALGSALTHPNITTDFSEAQLELITEVHQSPEATLAQLEGVHRFVYQNLDGEL
                     LWSASMPCVLGTDEEIPVGRYGGSNIARAKTIYRLGLGNRYGRLMQTISGIHYNFSVP
                     QTFWKVLAESRGK"
BASE COUNT         1721 a         3126 c         2956 g         1573 t
ORIGIN      
        1 gaccgtggtc gaggccacgt cgggcaacac gggcatcgcg ctcgccatgg tctgcgcggc
       61 cacgggacat ccgttcgtcg ccaccatggt ggagacgttc tccatcgagc ggcgcaagat
      121 catgcgcgcc ctgggcgcga aggtcattct cacacccgcc gcagagcgcg gcagcggtat
      181 ggtccgtcga gcggaggagc tggctgccga gcacggctgg ttcctggcgc gacagttcga
      241 gaacccggcc aatccggcct accacagaaa caccaccggc cccgagatcc tgaaggactt
      301 cgccgggcgc cggctggact actgggtgac cggctggggc accggcggca cgctgacggg
      361 cgccggggaa gtgatcaagg tcgcccgccc cgatgtgaag atcatcgccg ccgagccaga
      421 gcaggcatcc atgctggcag gcaaggagtg gagcccgcac atgattcagg gatggacgcc
      481 cgacttcatc cccgcggtgc tcaatcccga ggtgtgcgat gcgatcgttc ccgtcaatca
      541 ggacgtggcg aaagacaccg cgctggcgct ggcgcgcaac gagggcatct tcgtcggcgt
      601 ttccgccggg gggacgctcg ccgcggccct ggacgtcgcc gaggacgcgg cgcccggcag
      661 cgtgttcgtc gtgatgctgc cggacaccgg agaaaggtat ctttccacct tcatgttcga
      721 gggcatcaac gaaggttcgg acgacgactg gctcgagtcc ctgtgatcgg ccgccgagtc
      781 acgccgccga gctgccctga caaccggcag gtcggcgggc gaacggagag taacaatggc
      841 tgaattgagc ctcgaatcga tcttcacgcc cgagatcatc gcggacccct acccgctata
      901 ccggcagctt cgcgagacga gcccggttct cgagcttccc gacgccaaca tggtgattct
      961 cacccgctac gccgacgtgc agacggtcct gcgggatcgc cgcctgggcc acgccgacga
     1021 gtccatgctg acccggggcg atctcgagca ggcgcgaaac aacccggcca tcgccaattt
     1081 acggcgcacc atgctgctca agaacccgcc cgaccacacc cgcctgcggg gtctggttgt
     1141 caaggctttc gacgcgaggc gggtggaggc catgcgcacg cgcattcgag ccatagccaa
     1201 cgagctcgtg gacgcgttcg tcgatgccgg cagcggtgac ctggtccgcc tgttcaccca
     1261 tcctcttccg gtcatcgtca tatgcgacct gctcggcgtg cccaaggcag accaggccga
     1321 gttcgtgcag ggcacgcgca tttccggacg gctcatcgac ccgagcccca tgacggagga
     1381 agagctcgct caggcaaacg cgagcaccac ggagagccag cgttacttcg agcagctgtg
     1441 cgacgagcgc cgccaggcgc ccagtgacga cctgatcacc gccctcgtgc agagcgagac
     1501 cgagcacggc aagctcagca aggaggagct cacgtcgaac atctctttgc tgttcgcagc
     1561 cggccacgag accaccgtca acctgatggg caacgggctc atcgccctct accggaaccg
     1621 ggaccagctc gaccggctgc gcgcagaccc ctcgctcatg cccaacgccg tcgaggagtt
     1681 cctgcgttac gacagctccg tgcagctgac gggccgggac gcgctggaag acgccgagat
     1741 tgccggtatt gccctgccgc gcgggcgcgc cgtcctggcg ctgctggcag ccgccaaccg
     1801 cgaccccgag gcgttcgacc gcccggagga gctggacata gcccggaagc gcatcaagcc
     1861 ggtgtcgttc ggcggaggca tccacctgtg tctgggggcg cagctggcgc gaatcgaggc
     1921 ccacgaggcc ctgaacgtgt tgctcgaaag actgccgaat ctagagctgg acgacgtggc
     1981 cacgccggac tggaagcaga cgatcaccct gcgcggcgtg cgcacgctgc cggcaacctg
     2041 gtagtcggcg gcggagagcg tccccccctt agcgctccat gacctgtttg ccggcggccg
     2101 cgctcagcag ggtgacccgg tcgagatcga cgaccacggc aacgccaccc cggtccgggt
     2161 cgaactgccg ctccacgggg tgcatctcct gatacacccg ctcctgctct tcagcgtcct
     2221 ccgtcaccga ggcggtaccc tcgaaggtgt agtacacgcg accgccgatg tcaccgtaga
     2281 tgatggcgac gttgggccgg tcgggaatcg ttctgatcag atcgttctgc gggttgcgca
     2341 cccagatcgc cagctggttg ctgctgtgaa cgtgagtgct tccatagaag gagatgtggg
     2401 gcttgccgtc cacgtccacg taggccaccg tgacggtctt gtgttcctcg atggcccggt
     2461 ccagccggcc cttgatttcc tcgtccagct cgatcatcgc gtctcctcct ggggctttgg
     2521 ctgcccgtct ttggtctata ctcgcttgcc acgagggcga cccctgcgtc gccgaagcgc
     2581 cgtcgccgcc cgggcagacc gggggctgga gcgcttcgcg aggggggcga aacttaccat
     2641 actttcgatg cggattgccg gccgcggccc ggtcgtccac ggattggaca agcaatgcct
     2701 gagatactgt ttcttctcgt tgcaggacac gccatctgcg atttcgtcct gcagtccgaa
     2761 gccatggcga ccggcaagaa ccaccgcctc aaggagaaga tgcgggagcg gcacggcccc
     2821 tccttcccct cctggcctta ctggctgacc tcacacacga tcactcacgg ggccgccgta
     2881 ttcctgatca cgggaaatct gctgctgggg atgatcgaaa ccgtgctcca cacggtgatc
     2941 gactacacca agtgcgccgg tcggatcagc ctgctgaccg accagatcct gcacggcgcc
     3001 tgcaagatcg gctacgtgta ctacctttgg catgcgggcg cgctgggctg atccggcgct
     3061 ggccgccctc atcacgcggt cagcagcatc ccccagacca caaggcccag cgtgccccag
     3121 agaatgtacg ggcctacgaa agcgacgagc cgggcaatgg tctcgttcgc ccgaaatccc
     3181 ccctggtcct gtccgctcat caacttctcc cagctacgga cgcctgcgga gcccccaatt
     3241 gctccgcgtc tcggggcgat gtccggctgg ctgaagagag tggcgccggc ggaacgcccg
     3301 tcttctctag gttttatgcc cttcctaact atttggcaat taaatttacg tgagaggatg
     3361 tgcggcagat gtaacgtggt gctgctttgc ggaatcgggg ttcctgggaa aaatcgggga
     3421 aaagtgaaaa taatatggat tcgacaactt caactgattg atttcaaagg ctattgcctt
     3481 cgactagccc atgaggccgg ccagcaggct ttttttccgg aatgatttcg gtatattggt
     3541 ccacctcact agccggtgct gtatgtaaat acagtgtaat tctcctcggc agggcgaaca
     3601 cacgaacata agcccggcta gtgaggccaa cctcccgacc tttcaagctc tcccgaccac
     3661 tctacctgct ggactgactg acgctcttct ggagcccgtc tacggccttg ggcgctggtg
     3721 cccgctcagg cagcgggcac ggccggcaga accgttcgcc aggggtagga gccgtcggcg
     3781 gcggcgaaaa attccggatt gagaaaggat tccttcgcgt tgtaggagag cgctccgcca
     3841 cccaccgccc agacggcacc accggcagct tccagcaccg cgtgggctgc ggcgatgtcc
     3901 cattcagagg taggtcccag gcgaggatag agatccgcat gcccctcggc gagtatgcag
     3961 agcttcaacg agctgcccac gggcttgcgt tcgatctcgc cgatgctgcg ggcgagcgcg
     4021 tccaggtagt cctccagccg ctggccacca tggctccggc tggccaccac caccagagga
     4081 tcggagtcgt tgcgcttgcg tccgcgcagc ggcacggcgc cctcggggcc gtggcgcagc
     4141 gccgtcgcgg tggcaaggtc cccggtatac acatagccct gggtcggcac cccgacgacg
     4201 ccgaaaaccg cgcggtgacc ctcgatgagc gcgatgttga ccgtgaactc gccgttgcgg
     4261 ctcacgaact ccttggtgcc gtcgagagga tccaccagcc agtagcgcga ccagccctgc
     4321 ctcacggcaa actccggcgg gctggactcc tccgagagca cgggcacgtc cggcatcagc
     4381 tcggcgagcc ccgcggcaat gaccgcatgg gatgcaagat cggcctgtgt gagcggggac
     4441 gaatcgccct tgtgctccac gtcgaagtcc gtctggtaga tctccaggat cgcgtccccg
     4501 gcccgtcgtg tcaggtcgat cagcgcgttg gtcagctctg cgtccatctc gtctggctac
     4561 tcctcgttgt ccagtcgccc atcaagccaa ggccgcgcaa cgcttagagc ggctatggcg
     4621 cgggcctcgc gaaactcgtc gccggcaatg agctcgtcga gccgagccag cggccacgga
     4681 accagctccg gcggctccgg ctcgtcggca ggcaactgct cgggatagag atccgtggcc
     4741 agcatcacgt tgatggtgaa tcccatgtgg cccggcgcca cgctgaggca tttgagaaac
     4801 cgcacctgcc gggcgcccat gccgatctcc tccttgagct cgcgattggc cgcgtcctcc
     4861 agagcctcgt cccggttggc ggcgcccttc accatggtca gctgcatctc gtgaaagccg
     4921 gcgcaatatt cccggatcag caacagctcg ccacgctcgt tcaccggaac gacgatgacg
     4981 gccggcgtgc ccacacccgg caggcgttcg taggtgcgcc gctcaccggt ggcaaactcc
     5041 aggtcgatct cctcgatgcg gaagaaccgg gtggatgcga cggtgcgaac ggctttgatt
     5101 tccgggacgg gcacggcgaa ctctcctgct gctcggcacc aagtccgagt gcgaaaccgc
     5161 tattatacgg gccccgaacg gacagtgacg gcaaatgatc tgctgggact ccgtcgacac
     5221 cgtcctgctg gacatggacg gcactttgct cgacctgaat tacgacaaca ccctgtggaa
     5281 ccagctggtg ccgatgcgct acagcgaggc gaagtccatt cctgtcgagg ccgcccgcga
     5341 gctcctgttc gcacgcatgc tcgacacccg cgggcagctc gacttctact gcctggacca
     5401 ctgggcggaa ttcacgggcc tggacatcgt gggcctgcat cgcgagctta ccgacctgat
     5461 tcgctaccgc ccttatgcgc aggagttcct ggtgtggctg cgggctcgcg gaaagcgctc
     5521 cctgctggtc accaacgccc accgtgaaag cctgaaggtg aaggacgccc actccgacgt
     5581 gacgagcaag ctggacgcag acgtgtcgtg ccacgactac ggcgagccga aggagagccc
     5641 cgccttctgg gccgagctga tggcgaggca cccctacgaa ccggcacgca cgctgctcat
     5701 agacgacaac gacgccgtgc tgacggcggc cagagagttc ggcatcgggc acctgctcac
     5761 cgtggcccag ccggattccg ggcgaccccc cagggaaggc ctgggctatc cggcttttac
     5821 cgacttccgc gagatcctgc ccgatgagtg agggccaagc ggctcggctg gacagatggc
     5881 tgtgggcggc gcggttcttc aaaacccgcg cccaggccaa ggcggccatc gagggcggca
     5941 aggttcatct gcacctcaac cgcgacgaga gcggggcgac ggtccggccg aaggtcagca
     6001 gggaagtgag cgtcggagac gtggtcacgg tgaggcgagg gggagcgccc gagaccgtgg
     6061 tgatccgggc gatcgcccac cagcggggca gcgcctccgt cgccgcaacc ctctacgacg
     6121 aaacgccggc aagcatcgag gggcgagaag cccagcgcgc gcaacgccag ctggaacggc
     6181 tcgggctcaa ggtgccgagc accaagccgg gcaaacgcga tcgtcgagcg ctcaagaagc
     6241 tcaaggaaga ttcggcgccg tcatgaccgc ggaccgcctg catcgtttca gctttcagaa
     6301 cgcgccgatt cgaggcaact gggtgcgtct ggacgccgtg ctggccgagg tgttcgcacg
     6361 tcagtcctat ccgggccggt tgcaggcggt tctggccgaa gccctgagcg cggtcgccat
     6421 gctcgccgac accgtcaagt tccaggggac catcagcctg cagtcccgag ggcggggacc
     6481 agttacaacc ctgctggcag agtgccgctc gcgaagcgtg ctgcgcggga tcgctcgatg
     6541 ggaccgatcg gccggcattc cggaagacgc gcccctgggt gagctcctcg gtgacgctcg
     6601 gctggccatc tctctcgtgg actcggatcc ggccacaccc cgtccggtga gctatcaggg
     6661 tctggtcacc ctggatcacg aaagcctggc cggcaacctg gaaggctact ttgccttcag
     6721 cgagcagctg cccacacggt tctacttcgg cagggcggga cacaccgtca ccgggctgct
     6781 gctgcagcgg ctgcccgccg acgacgacgc cacggagatc gatctcgacc agcacgaatc
     6841 ggattggaaa cagatccagg aagccgcagg cgagtcctcc gccgacctgg ccgcgttgcc
     6901 cccgcaagcg ctgttgaggc gtttgtttcc cagccggccg ctgacgctgc aaccgggacg
     6961 gccgctgtct ttcggctgca cctgcagccg cgatcgcagc accgcctcac tgaacgcggt
     7021 gccggccccg gagctgctgg acattctgca cagcgacggc gaggtcacgg tgacctgcga
     7081 gatctgcggc accgtctacg cctacgaccc catcgacatt cacctgctgc tgcaaaggga
     7141 agccccgcag cttcactgag cgagcttccc ttacaatccc tttcattccg aataggccaa
     7201 atctcaccgc tctggcataa tcgatcgatt gtcaggggaa gacgctgaaa gcatggcagt
     7261 cgacatagag tcaacggcaa agcacaccac tcatctcaac cttgccgccg ccgcgcttgc
     7321 cgaactggcc gtattgaggg gggagggtca attcgccagc aacggcgcca tcgtcgttcg
     7381 aaccggtcat cgcaccgggc gctcgccgag ggatcggttc atcgtggagg agccgtcgac
     7441 ggccgacgcc atcgactggg gaaccataaa ccagcccatc gcgcaggacg tattcgacgc
     7501 gctctgggac cgggtgcaga ttcacctgga ggaccgcgaa cggttcgttt ccaacctcca
     7561 cgtcggggcg gacccggaac actatctgcc catcgaggtg actacggagt acgcgtggca
     7621 cgcgctgttc gggcgggcac tgttcatcaa gccggagaac tacaaccccc acaacaagtc
     7681 cgtgtggaac atcgccaacg caccgagttt cgtctgtgac cccgagcgcg acgggacggc
     7741 cagcgacggg acggtgatga tcaactttgc tcagcgcaag gtgctgctcg ccgggatgcg
     7801 ctacgcgggc gaaatgaaaa aatccatgtt ctcggtgctc aacttcctcc tcccggagaa
     7861 ggacgtcctg cccatgcact gctcggcaaa catgggccac gacggtgcgg tgacgctgtt
     7921 cttcggcctg tccggcaccg gcaagacgac cctgtcggcg gatcccgacc ggctgctcat
     7981 cggcgacgac gagcacgggt ggggcaaagg aaccgtcttc aacttcgagg gcggctgcta
     8041 cgccaagtgc atcgacctga cccgggagaa tgagccgctc atctacgatg cgatccggtt
     8101 cggcgccatc gtcgagaacg tcatcatcga ccaggacacc cgcgaacccg actacacgga
     8161 cggatcgctg accgagaaca ccagggcctg ttatccgcgc agcaacatcg aagccaaggt
     8221 gaccgacaac cgtggcggcg aacccggcca ggtggtattt ctcacctgtg acgtgagcgg
     8281 cgtgctgccg ccggtttccc tgctctccaa ggaagctgcc gcctaccact tcctgtccgg
     8341 ctacacggct caggtggggt ccacggaagt gggctctacg gaagcctaca aggcaacctt
     8401 ctccacctgc ttcggggcgc ccttcttccc gcgtgcggcc ggtgtgtacg ccgatctgct
     8461 gatcaagcgg atcgaggagt tcggctccaa ggtgttcctg gtcaacacgg gctggaccgg
     8521 cggcggctac ggcgttggca aacgcttcgc catcccggac acgcgggcca tcatccgggc
     8581 cattcagtcc ggcgccctgg acgacgccga gacccggcgc ctgccaggtt tcaacctgga
     8641 cattccgaca tcggtgccgg gcgtggacgc aggtcttctc aacccgagag atacgtggtc
     8701 cgacaaggcc gcttacgacg aatccgcgcg cagcctgatc gcaaaattcg cagagaactt
     8761 cgaaaagttc agcgtcgctc aggccatcgt tgatgctggt ccgaccacgg attattgagg
     8821 tagcctgaag gccgccccgt tgggctaaga ggcggatatg gcccacgcag ccgcacccat
     8881 cgacccggcg ctcctggacg cattgcgcga ccagccggct tccctgcgag agatccagcg
     8941 gggcatcgag aaggaaagcc tgcgggttac gccggaaggc tcgctctcag accgaccgca
     9001 tccggccgcc ctgggctccg cgctcaccca tccgaacatc accacggact tcagcgaggc
     9061 tcagctggag ctgatcaccg aagtgcatca gtctcctgag gcgacgctcg cccagctgga
     9121 gggcgttcac cggttcgtct accagaacct ggacggcgag ctgctctggt ccgccagcat
     9181 gccgtgtgtg ctcggcacgg acgaggagat tcccgtaggt cgctacgggg gctcgaacat
     9241 cgccagggcc aagacgattt accggctggg gctcggtaat cgctacgggc ggctgatgca
     9301 gaccatctca ggcatccact acaacttttc ggtcccgcag acgttctgga aggtgctggc
     9361 ggaaagcagg gggaaa
//
LOCUS       DBNHLM010000339         6438 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_350156, whole genome shotgun sequence.
ACCESSION   DBNHLM010000339 DBNHLM010000000
VERSION     DBNHLM010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6438
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_350156"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="AC16QF_15685"
     CDS             complement(<1..1268)
                     /locus_tag="AC16QF_15685"
                     /inference="COORDINATES: protein motif:HMM:NF024825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HIR6450051.1"
                     /translation="MQVPFPHHSVRLRFALLATLLATGCGGDTDAPSFDGDVRVGILH
                     SRTGTLALSENTVAEAELLAIREINEMGGIQVGNARLRIVPVEEDGQSDPSVFATRLS
                     RMIDQDGVKVVFGGWTSASRKAMVPVLEARDHLLFYPIQYEGEECSANVLYAGATPNQ
                     QAGPAIEWLLENRGRRFVLAGSDYVYPRTANAIIRRQIAALGGEITGEFYIDLGEMSL
                     EPLVRNIEASLPSGGVIVNTINGDSNVAFFESLQRAGITGDAGYSVMSFSLAEEEIAA
                     MGAQAMVETLAAWSFFQSLETPGSIEFTRRFRDAYGAHRVTSDPAATGYSMVHLWAAA
                     VEKAGSVEPEAVRDALPGVTIASPLGELAVLSNHHVRKATLIGEVQPDGQFKILHAEE
                     PIIPTAWSPWLPENEGFVCDFSQERPDASR"
     gene            complement(1575..2432)
                     /locus_tag="AC16QF_15690"
     CDS             complement(1575..2432)
                     /locus_tag="AC16QF_15690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HIR6450052.1"
                     /translation="MIELSNGHSVWSKTVGDGSGLPLLLLHGGPGAGHDYLEPLEVLS
                     SERPVIFYDQLGCGKSDKPSSKALWTIERFADEVQEVREALGLDRCHILGQSWGGWLG
                     IEYVLRQPEGLASFVAASTSASVREFSANCDRLIAQMPEKHRDALHHYGARGEIEHED
                     YVAAEEFFYSEHLCRLEKWPDCLGRTIENLDGNDVYYTMNGPNEFATIGNLRYWDRTA
                     DLDQIKVPVLITCGRYDELGPTCAATLQDGIENAQTVIFEESAHVAHIEEAEAYARCL
                     SEFLKAHDS"
     gene            3478..4491
                     /locus_tag="AC16QF_15695"
     CDS             3478..4491
                     /locus_tag="AC16QF_15695"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6450053.1"
                     /translation="MKDVRAVYHETPGQTADTILLTLEYNGDTRLPDRMFGKVNTRNA
                     DTLDIVKSMDLYRREVAFYNQVEDVGLPVPECYYGSFDPENYQFVLLLEDLSDGICPS
                     WGISEAQARDAIALLPSFHARWWNSSEARRFDWAIQLDEAVYWNNWRADTPVNLERAI
                     AKLGPALTDDFIATVRLALDKWEALLAYLATRPFALVHGDYHFKQIFFPTAEGAGRLA
                     VFDWQFPYVAPGPWDLARVMGATLSSQTRRSLQPEILAGYLNGLARCGVAGYDMDGLH
                     EDLATGSLINLLIHTNAFGATDLSILEREAASYGADMRQVLIDQMVDLAADFDTAGLL
                     RGL"
     gene            complement(4697..5167)
                     /locus_tag="AC16QF_15700"
     CDS             complement(4697..5167)
                     /locus_tag="AC16QF_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450054.1"
                     /translation="MVERLNHWLALIGNLGVIAGIVFLAYEIQANTNAVRSATYQAFN
                     DSSFSWADSAIENAAIMAKAAANGSLAELSPKERIIWDRFMFKTFTVMESNYLHHRAG
                     SMDDDVFESKISGSIGAILNEPLWIESWNRAGFLLPEFKEYMNSRLRATKISGS"
     gene            5437..5982
                     /locus_tag="AC16QF_15705"
     CDS             5437..5982
                     /locus_tag="AC16QF_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450055.1"
                     /translation="MSWENWLALGTNVAVFLGLLLVVLELNQNSELARVELINEGNAI
                     ENELWQSLMDQVPKDAIAKSIECPEKLDLSDYVVLDSYLYTAMNLVYRNYELSKEGLF
                     SQDDWRSEVDNYAHWYLSGEFGRAYWDNAGRDYFDSEFSEYVDSVLEKDGIDIGEAWT
                     KIASALPSKNGTVPTTSSICM"
BASE COUNT         1341 a         1882 c         1861 g         1354 t
ORIGIN      
        1 aaccggctcg catcgggccg ctcctggctg aagtcacaca caaacccctc gttctcggga
       61 agccacggcg accacgctgt ggggataatc ggttcttcgg catgcaggat cttgaactgg
      121 ccgtcgggct ggacctcacc gatcagtgtg gccttccgga cgtggtggtt gctcaggacg
      181 gccaactcac caagcggcga cgcgattgtg acgccaggaa gcgcgtctcg cactgcctcc
      241 ggctccaccg agcccgcctt ctcgaccgct gcggcccaca gatggaccat gctgtacccg
      301 gtggctgccg ggtcgctcgt cacccgatgt gctccgtacg catcacgaaa ccgccgggtg
      361 aattcgatcg accccggcgt ctccaggctc tggaagaaac tccacgcggc aagagtctct
      421 accatcgcct gggcgcccat cgccgcaatc tcttcctccg ctagtgagaa actcatcacg
      481 gagtaacccg cgtcacccgt gatccccgct cgttgcaggc tctcaaagaa ggcaacgttg
      541 gagtctccgt tgatcgtgtt cacgataacg ccgccgctgg ggaggctcgc ctcgatattc
      601 cgaacgagcg gttcgagact catctcaccg agatcgatgt agaactcccc ggtaatctct
      661 cccccgaggg cagcgatctg ccggcggatg atcgcgttgg cggtgcgagg atagacatag
      721 tcagaaccag ccaggacgaa acgccgcccc cggttctcga gcagccattc gatcgcgggg
      781 cccgcctgct ggttgggagt cgcgccggca tacaggacat tggcggagca ctcttccccc
      841 tcgtactgga tcggatagaa gagcaagtgg tcgcgagcct cgagaaccgg gaccatggcc
      901 tttcggctcg ccgacgtcca tccgccgaac acgaccttga ccccgtcctg atcgatcatc
      961 cgggacaggc gcgtcgcgaa gacagatggg tcagactgcc cgtcttcctc gaccggaact
     1021 atcctcagcc gggcgtttcc gacctggatt cctcccatct cgttgatctc tcgaatggcg
     1081 aggagctctg cctccgcgac cgtgttttcc gaaagagcga gtgtcccggt tcgggagtgg
     1141 agaataccaa cgcgcacgtc cccgtcgaac gacggtgcat ccgtgtcacc gccgcagccc
     1201 gttgcaagca acgtggcgag cagcgcgaac ctaaggcgca ccgagtggtg agggaacggt
     1261 acctgcatgt gagctggagc tccggatcag ttggtagaga ttctacatag tctgggacga
     1321 aagatggcct gaaacgaggt gaaataagtc cgatttagac tgtggattaa actggtcaac
     1381 gcaacagcct agtctcctgt tagggaaaag gagtgttgca gttgaaggga agacgcgggc
     1441 gctattacag tgaagggcgg aaggtgcaga tgtgggaccg ctggcagccc ggcgagtcat
     1501 tgaattcgat tgcacgatca ttctaggcca gccattcagc gccggtgata tgcgcagcaa
     1561 gcgtatgctg atttctatga gtcatgcgcc ttcaggaatt ccgataagca tctggcatac
     1621 gcctccgctt cctcgatatg ggctacgtgg gcactttctt caaatattac ggtctgggcg
     1681 ttctcaatac cgtcctgcag ggttgctgca cacgtcgggc ccagctcatc gtagcgccca
     1741 caggtaatca gcacgggcac tttgatctgg tcgaggtcgg cggtgcggtc ccagtagcgc
     1801 aggttgccaa tcgtggcgaa ctcatttggg ccgttcatcg tgtaatagac gtcgttgcca
     1861 tcgaggtttt caatagtacg tccaagacaa tccggccact tttctagccg gcacagatgc
     1921 tcagaataga agaattcttc agctgccacg taatcttcgt gttcgatctc gccgcgggcg
     1981 ccatagtgat gcagcgcatc gcgatgtttt tccggcatct gcgctatcag gcggtcgcag
     2041 ttagcagaaa actcgcgaac agaggccgag gttgaggcgg ctacgaaact cgccagtcct
     2101 tccggctgac gcaatacgta ctcgatgccc agccatccgc cccagctttg gccaaggata
     2161 tggcaccggt cgagacccag cgcttcgcgc acctcctgca cttcgtctgc gaagcgttca
     2221 atggtccaca aagctttgct ggacggcttg tcagatttac cgcaacccaa ctggtcataa
     2281 aagatcacgg ggcgttccga gctcagcacc tcgagcggct cgagatagtc gtgccctgca
     2341 ccgggacctc catgcagcaa gagtaatggc aaacccgacc catctcccac ggtctttgac
     2401 cagacgctgt ggccattgga aagttcaatc atgcgttcca gaacagttct ccttcatgaa
     2461 ctcgatgcgc caaccgtaac gtaccatagg attattgcga cgaggcagga cgaaaccgtt
     2521 agggccgcct gcgttccctc cagattggcg gttaagaagc cggtctgaac agcgatctaa
     2581 cgtctacgcg ccaccaatcc ggagcacaac tctgtccgct ccggatcgga agcggccatc
     2641 accggccata ccgcggaacg tctgctatcg ggcgggaagc ggacgtggcc attgtgcagt
     2701 gcaatgaatc gctatcggcg attaggcgac acattggagc gtcgtagatt cacttgaatt
     2761 aaggtgccgc gttagcaagg gccgccaaga tatcttcagt cctacatttg cgccctggag
     2821 acgatgccag gagcgcaaag tattacgctt cttcacgcca catttcgtcg agattccagc
     2881 ccactatacg gcccatgaac cgtgcagtgc cgacggcccc agaattggat actcggttct
     2941 ggcgaagctc ttcgaggcgc ctttcggtgt aatcaccgct ccaatgggta agatgtgttt
     3001 tccctttagc aatgaatcga gttcggaccg tccatgcgta cggtgtgcag tgtttcagcg
     3061 caaagtccgg cgtaaactct tgacccagaa tgccgcggga ttcatgcagc atcgcgcctg
     3121 gaaccagcaa tatgggcgtc tcggtcgaat gggggaacaa taatggatcc gcgttccccg
     3181 acatccgcta tcggaaggga gcggacttca acatcctgcc attcagcccc caattggtaa
     3241 tcggttcgca accggaggga tggtgcatgc tggaatatag gagctgtgca ttagtcggcc
     3301 cgaatggtcc aatcgttgtt ccagctttcg cgtctcacgg caccgatgct actcttgcgc
     3361 gcgcttcatc ggtgatcaga ggggggatga ttggtgatac ctgccaagcc acaagatttc
     3421 acggtcgact ggttgcagag cgccctggcc gcccggctag cggagagtaa tgcgtacgtg
     3481 aaggatgtgc gggctgtcta tcacgagact cccgggcaga cggcggacac cattctgctg
     3541 acgttggagt acaacggaga tacccggctg ccggacagaa tgttcggcaa ggtcaacacc
     3601 cgcaatgcgg acacgctcga catcgtgaaa tccatggacc tgtaccgccg cgaggtggcg
     3661 ttctacaacc aggtcgaaga tgtgggcctg ccggttccag agtgctatta cgggtcattt
     3721 gacccggaga actaccagtt cgtcctcctg ctggaggatc tgtcagacgg tatctgcccg
     3781 tcctggggta tctccgaagc tcaggcgcgc gatgcgatcg cgctgctgcc gtccttccac
     3841 gccaggtggt ggaacagctc cgaggcgcgc cgcttcgatt gggcaattca gctcgacgag
     3901 gcggtctact ggaacaactg gcgcgcagac acgccggtca acctcgagcg ggccatcgcc
     3961 aagctcggcc ccgccctgac cgacgatttc attgccaccg tgcgcctcgc tctggacaag
     4021 tgggaggctc tgctcgccta tctggcaacc cgcccattcg ccctcgtgca cggggactat
     4081 cacttcaagc agatattctt tcccaccgct gaaggcgccg gtcgactggc cgtcttcgat
     4141 tggcagttcc cctacgttgc gcccgggccc tgggatctgg cgcgggtgat gggcgcgacg
     4201 ctgtcctccc agacccggcg cagtctgcaa cccgagatcc tggcgggcta tctcaatggg
     4261 ctggcgcgct gcggcgtcgc gggctacgac atggatgggc tccacgagga cctggccacc
     4321 ggcagcctca tcaacctgct gatacacacc aatgccttcg gcgccacaga ccttagcatc
     4381 ctggagcggg aagccgcctc ctacggcgcc gacatgcgcc aggtactcat cgatcagatg
     4441 gtcgatcttg ccgcggactt cgacaccgcc ggcctgttgc gcgggctttg aagtgtgttc
     4501 ccgagcttgg gcgccgtccc gctggtagga acggtgggga cgcgagcgaa cctcagcatc
     4561 cccgcctcgg attgtccgct ttagagcggg acgcggacgt tttggttcgt gcccgctaac
     4621 ttccgctatc ggccaggagg gaagtgccta cgccggggtc tgcagtaacg ccggcaagat
     4681 gtgcgacacg accgccttaa ctgccgctga ttttggtagc tctgaggcgc gagttcatat
     4741 actccttgaa ttccggaagc aggaaccctg cgcggttcca agattcaatc cacaaaggct
     4801 cattcaatat ggctccgata gatccactga tcttcgactc aaagacatca tcatccatgg
     4861 agcctgcgcg atgatgcaga taattagatt ccataacggt gaatgtcttg aacataaagc
     4921 ggtcccatat gattcgctct ttcggggaca actcagccaa actgccgttc gcggccgcct
     4981 tcgccataat cgcagcgttt tcgattgcag agtcggccca ggagaaacta ctgtcattga
     5041 acgcctgata ggtggccgat ctcacggcgt tagtattcgc ctgtatttcg taagccagga
     5101 aaacgatccc agcgattacc ccaaggttgc cgattaacgc caaccaatga ttgagtcttt
     5161 ccaccatctt ctcccgcttt tcggattgct cacgactatc gtacttcagg aacggcgcaa
     5221 gcgaatggcg gctttggagc gcgggcgctg gtattccgac ttcaccgaaa gcggcagcta
     5281 cgagtgagcg cggtcagcac ccgggttcga cctcgtgccc tgtcggccaa cagcagcctc
     5341 ttgccgattc cgttgtaagc tacgcagatc gagctggtca gcgggcttgc cgtagccacg
     5401 gtaccctgaa gacgtgcgtg tagtggtagg tatcagatga gctgggaaaa ctggttagca
     5461 cttggaacaa atgtcgcggt gttcctgggc ctccttctgg tcgtgctgga gctgaaccag
     5521 aattcagagt tggcacgcgt agagttgatc aacgaaggta acgccatcga aaacgaactt
     5581 tggcaatcct tgatggatca ggtgcctaag gacgccattg caaaatcgat cgaatgcccc
     5641 gagaagcttg atctgtctga ctatgtggtg ttggactcgt atctctatac agcgatgaat
     5701 ctcgtctacc gaaactacga actgtcaaag gagggccttt tctcgcagga cgattggagg
     5761 tcggaggtcg acaactacgc acattggtac ctgtctgggg agtttggaag agcatactgg
     5821 gataacgctg gacgcgatta ctttgattcc gagttttcgg agtatgtaga cagcgttttg
     5881 gagaaggacg gcatagacat aggtgaagcg tggacgaaga tcgcgtcggc actcccgagc
     5941 aagaacggta ccgtacccac gactagctcg atatgcatgt gatcgcccac gagcattacg
     6001 tagtgggcgg tcacgcatgg acggctaggt caaaagtggt ttagggtcgt aagcaggcat
     6061 caccgcccag ggtccggaac gtctgctttg gagcgcgaag ccgacgtcgc tattgtgccg
     6121 cgcaatagac cgctaccggc cgtggccgtg tgagaactcg tggatttcgg ttcggacact
     6181 ggcgcgtatc cacttccacg gctggcgcag cgctagattc gcgagattgc gttcgatttt
     6241 ctcaaggcaa acgatctcgt tagatcgcct cgatgatctt ctgcgctccc agtaggttga
     6301 atccgcagtc aattgcagac gcctgctaat ctggtggtag catggccgcg gacacattta
     6361 caactatgac actgcgggct ggaacggcct aggattcaat tcgtgacgat ccctgattcg
     6421 ccgacatgct cgaactgc
//
LOCUS       DBNHLM010000340        12136 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1368037, whole genome shotgun sequence.
ACCESSION   DBNHLM010000340 DBNHLM010000000
VERSION     DBNHLM010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12136
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1368037"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="AC16QF_15710"
     CDS             <1..654
                     /locus_tag="AC16QF_15710"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF017509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR6450056.1"
                     /translation="EVTEGKLPELDDEFFAAFGIEEGGVDAFREDVRNNMQRELDNAV
                     ASQVKTQVMDQLNELHTVQLPKAVVKQEIEALKAQMLQQFQMPGTAAGGLPDLPDELF
                     AEQAQRRVAVGLIVSELVSRKSLEADADKVRARIQSIAEQYAEPQQVINWYYGNEDQL
                     RQIEMAVLEEQVIDEVMEQAAVEAVESSYNDIISGRAFAAPEAEPDEEATETSGRIE"
     gene            651..1301
                     /gene="clpP"
                     /locus_tag="AC16QF_15715"
     CDS             651..1301
                     /gene="clpP"
                     /locus_tag="AC16QF_15715"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIR6450057.1"
                     /translation="MSQIGDANIPEITNLGLVPMVVEQSARGERAFDIYSRLLKERIV
                     FMVGPVEDNVANLVVAQLLFLESENPDKDIHLYINSPGGSVTAGLSIYDTMQFIRCDV
                     STMCIGQAASMGAFLLAAGAKGKRYALPNSRMMLHQPSGGGRGVAADIEIQAKEILLM
                     REQLNRLLADHTGQSVERIATDTDRDYWMSPDQAAEYGLVDMVQRPRSDVAKAVNE"
     gene            1446..2726
                     /gene="clpX"
                     /locus_tag="AC16QF_15720"
     CDS             1446..2726
                     /gene="clpX"
                     /locus_tag="AC16QF_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR6450058.1"
                     /translation="MSDQSGKGDDSGKLLYCSFCGKSQHEVRKLIAGPSVFICDECVE
                     LCNDIIREEVSEGGAAKESDRLPVPQEIKDILNQYVIGQEHAKKVLSVAVYNHYKRLN
                     YTGKKDDVELSKSNILLIGPTGSGKTLLAETLARLLDVPFTIADATTLTEAGYVGEDV
                     ENIIQKLLQKCDYDVERAQVGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIE
                     GTVASVPPQGGRKHPQQEFLQVDTSNILFICGGAFAGLDKVITDRSDKSGIGFGAQVK
                     GEEDRKSIGETLRRVEPEDLIRYGLIPEFVGRLPVVATLEELDTDALVQILTEPKNAL
                     TKQYTKLFEMEGVEVDFREDALRAVAQKAMERKTGARGLRSILESVLLDTMYDLPSSD
                     SVTKVVIDESVIKGESEPMLMYENVEKAQAAPKE"
     gene            2840..5269
                     /gene="lon"
                     /locus_tag="AC16QF_15725"
                     /pseudo
     CDS             2840..5269
                     /gene="lon"
                     /locus_tag="AC16QF_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365214.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
     gene            5405..5677
                     /locus_tag="AC16QF_15730"
     CDS             5405..5677
                     /locus_tag="AC16QF_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016782315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR6450059.1"
                     /translation="MNKTELIDRIAESADISKAAAGRALDAAIDAITDSLKQADPVSL
                     VGFGTFTVRDRAARTGRNPQTGAEIQIAAARVPAFKPGKALKGALN"
     gene            5782..7680
                     /locus_tag="AC16QF_15735"
     CDS             5782..7680
                     /locus_tag="AC16QF_15735"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HIR6450060.1"
                     /translation="MLQKLRDQTQSFGFKVLAGVMIFVLAVFGFGAFNLFVNSDPEVA
                     SVNGEEITQSELLVAMEREQGRIAAQFGEGFDPSLIDGARLQAAVLDQLIARQLLGQA
                     VDELGFGVSQSRVDEAVTANPAFQVDGRFDAELYKRRVRQLRYTPQQFHRETGALMAL
                     EQLRRGLTETAFLTDWEVRQSARLLSQKRDIAYLAFTEDDFLPLVEVSDEEVATRYAE
                     NELEYRTEETVDVEYLELSAEGLVNDESISVSEAAIEETYAADSAEALSGDRRQARHI
                     LLEVGASRTAEQAENELNAVRERIVGGADFASLAEELSEDPGSAALGGDLGMVAKGIF
                     DPAFEQALWELEVGEISEPVRTDFGYHLIQLDAVDVRELPPLSEQRDDIELRLRREQA
                     EELFSERLREFDNLAFEQPDGLEGIAAALALETQSVAGVSRSRGDGVFANASLRDAVF
                     ASEVLDNGYNTQAVALTDNRAVVARVKARYEPEAIPLDDVRDDIRAAIASEKARVALR
                     QAQQNALARVRSGESVSAVADDYGLGWQTFELVSRSSADAPRAVLSAAFSLPRPGERE
                     KSVGEATLDDGSVAVVTVTRVVDGDVSVMAEREIESMRGFLGNRVASLDFAGLTTTLE
                     REASISTP"
     gene            complement(7711..9258)
                     /locus_tag="AC16QF_15740"
     CDS             complement(7711..9258)
                     /locus_tag="AC16QF_15740"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /protein_id="HIR6450061.1"
                     /translation="MTATAPVVRFASTLRTLMFVILASLVGCQTNPIPEAAETAAAEP
                     PPPVAVPPPVIEPTLTYELKPDLWRDIRERFELAHSLDQKRVQQELRWLRENPRYLHN
                     LKPRMERFLAYIHGEVAARELPGELALLPIVESALDPYAFSHGGAAGLWQFIPATAKR
                     FGLQRNWWYDGRRDPIAATQAALDYLEYLHLLFEDWHVAVAGYNAGEGNVRRAQRKAA
                     PGATFWEMALPRETQMYVPRLLALAAVVTDPEAYGLTLPELSPDVPFVAVDTQGQFDL
                     AKAADTLSMNLDALYDWNPALNQWSTPPDGPHRLMIPALEPALSQAALAAVPAGERVQ
                     WLRIKVADGDTLSEIAHRHRTDTATLRRVNNLTGSSIRAGQAMYIPKSDSAPSRSPRS
                     RAQKGLEYAVQPGDSLWTISRAHGVSMTRIMKANHVGPKDLLKVGQTLTIPGAQRSTP
                     AAGEDGREVIRTVRYGVRKGDSLARIAARFNVRVSEIAQWNQLDVDNYLQPGQSLKLH
                     VNVAAGQ"
     gene            9333..10115
                     /locus_tag="AC16QF_15745"
     CDS             9333..10115
                     /locus_tag="AC16QF_15745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6450062.1"
                     /translation="MSAWLATPVGERLLADEAPYVAETARRFHGDTLLWAGCHTPLTD
                     TVRGCMVRNRVFALDGSSPAGEGLTTFRCELDAIPLANNALDGVLLHHALEMAEDPRT
                     ALRELSRVLAAGGRLTVVGFNPFSFWGVRIAYARVFRDSFSGLRLVSPARLVDWLTVL
                     GFELCEEVRFTAYNLPFRLGNTGEQDERSTQQLIPSSWGPRLRNALDHLNPPVGGVYM
                     VSAVKQALGVRRDWRLSAAREPKLAPVAYPKLSARNRIERSR"
     gene            10099..10551
                     /gene="rnhA"
                     /locus_tag="AC16QF_15750"
     CDS             10099..10551
                     /gene="rnhA"
                     /locus_tag="AC16QF_15750"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HIR6450063.1"
                     /translation="MSAPVEIFTDGACRGNPGPGGWAALLRYGDAEKLISGAAPDTTN
                     NRMELQAAISALEVLTRPSSVAITTDSEYLRQGINQWIQKWKRNGWRTSQRKPVKNRD
                     LWQKLDELVQQHQIEWHWVRGHSGHPENEQVDEAANVAIDRMLTGGES"
     gene            10548..11267
                     /gene="dnaQ"
                     /locus_tag="AC16QF_15755"
     CDS             10548..11267
                     /gene="dnaQ"
                     /locus_tag="AC16QF_15755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HIR6450064.1"
                     /translation="MTRQIVLDTETTGLEAQKGHRVIEIGAVEMIDRKPTGRHFHKYI
                     NPDRDIDDGAFEVHGLSSEFLSDKPRFEDIAQEFLAFVSGAELVIHNAGFDVGFLNYE
                     LAMLEAVPDGIESVCLVTDSLSLARSKHPGQKNNLDALCRRYGVDNSARTLHGALLDA
                     EILADVYLAMTGGQTALFGSEDAVGAGVDRDQAVASFDGQRPRLKVILATDEEIQAHE
                     AVLDFLEQKSGDCVWRQPSGS"
     gene            complement(11268..>12136)
                     /locus_tag="AC16QF_15760"
     CDS             complement(11268..>12136)
                     /locus_tag="AC16QF_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019579219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR6450065.1"
                     /translation="TALGGVCTTVGEPQNLLIAGQVGWEFMEFFLRMAPVTMPVLVVG
                     LTTCVVLEKARAFGYGTDLPGPVRDILERFDQAEAEARSGRDKARLVIQAVAAMLLVV
                     ALAMHWAEVGLIGLMVIVIQTAFNGVIEEHNLGRAFEEALPFTSLLVVFFGIVAVIHD
                     QHLFAPVIDYVLTLEVSVQPGMFYIANGVLSAISDNVFVATVYINEVTNAFEEGQIGR
                     EHFENLAIAINTGTNLPSVATPNGQAAFLFLLTSSIAPLVRLSYSKMVFMALPYTIVL
                     GITGWFGVQYLL"
BASE COUNT         2419 a         3589 c         3971 g         2157 t
ORIGIN      
        1 gaagttaccg agggcaagct tcccgagctg gacgatgagt tttttgcggc gttcggcatc
       61 gaagaaggag gcgtggacgc gtttcgggaa gacgttcgca acaacatgca gcgcgaactc
      121 gacaacgcgg ttgccagcca ggtcaaaacc caggtaatgg atcagctcaa cgagcttcac
      181 acggtccagt tgcccaaggc cgtggtcaag caggaaatcg aagcgctcaa ggcccagatg
      241 ctgcagcagt ttcagatgcc gggtaccgct gcgggcggtc tgcctgacct tcccgacgag
      301 ctgttcgccg agcaggcgca acgtcgggtt gccgttggtc tcatcgtcag cgagctggtg
      361 tccagaaaaa gcctggaagc cgacgccgac aaggtgcggg cccggatcca gagcatcgcc
      421 gagcagtacg ccgagccgca gcaggtcatc aactggtact acggcaacga agaccagttg
      481 cgccagattg agatggccgt cttagaggag caggtcatcg acgaagttat ggaacaagcc
      541 gcagtagagg ccgtcgagag cagctacaat gacatcattt cgggtcgagc cttcgcggcg
      601 ccggaagcgg agcccgacga agaggcgacg gaaacctcag ggagaatcga atgagccaga
      661 ttggtgacgc caacattccc gaaatcacta acctgggcct ggtgcccatg gttgttgagc
      721 aatctgcacg tggcgagcgg gcgttcgaca tctattcccg ccttctgaag gaacgcatcg
      781 tgttcatggt cggtcccgtg gaggacaacg tggccaacct cgtggtggcg cagctgctgt
      841 tcctcgagtc ggaaaatccg gacaaagaca tacatcttta cattaactcc ccggggggat
      901 ccgtcaccgc tggattgtcc atttacgaca cgatgcagtt catccgctgc gacgtcagca
      961 ccatgtgcat cgggcaggcg gccagcatgg gcgccttcct cctcgcggcc ggagcaaaag
     1021 gcaagcgata tgcgcttccg aactcccgca tgatgctgca tcagccgtcg ggtggcggcc
     1081 gcggcgtagc ggccgacatc gagattcagg ccaaggaaat attgctcatg cgggagcagc
     1141 tgaaccggct tctggcggat cacaccggtc agagcgtgga gcgaattgca acggatacgg
     1201 atcgggacta ctggatgagc cccgaccagg cggctgaata cggtctggtg gatatggtgc
     1261 agcgaccacg cagcgatgtc gcgaaggctg tcaacgagta aggggatcga aaagccgctt
     1321 gcatctgagc cacatggcaa gacttgcatt atcatttctg aaatcgcaag gtagcgaagg
     1381 tcaaaagcac gcagcgccag ggtgcggcgt ggttcggcgg agacaggcag cagacgaggt
     1441 tttctatgtc cgaccaaagc ggtaagggcg acgactcagg caaattgctc tactgctctt
     1501 tctgtggaaa gagccagcat gaggttcgca agctcattgc cgggccatcg gtgttcatct
     1561 gcgatgagtg cgtggaactt tgcaacgaca tcatccgtga ggaggtgtcg gaaggcggcg
     1621 ccgccaagga gagcgatcgc ctgcccgtac ctcaggagat caaggatatc ctgaaccagt
     1681 acgtcatcgg ccaggagcat gccaaaaagg ttctttccgt ggcggtctac aaccactaca
     1741 agcggctgaa ctacacgggc aagaaggacg atgtcgagct gtcgaagtcc aacatcctcc
     1801 tcatcgggcc cacgggtagc gggaagacgc tgctcgccga gaccctggcg cgactgctag
     1861 acgttccctt caccatcgcg gacgcaacga cgttgaccga ggcgggctac gtcggcgagg
     1921 acgtcgaaaa catcattcag aagctgctgc agaagtgcga ctacgacgtc gagcgcgccc
     1981 aggtgggcat cgtctacatc gacgagatcg acaagatttc gcgaaagtct gacaacccct
     2041 ccatcactcg cgacgtgtcg ggtgagggcg tgcagcaggc cctgctcaag ctgatcgagg
     2101 gcaccgtcgc gtcggtgccg ccccagggcg gccgcaagca tccgcagcag gagttcctgc
     2161 aggtcgacac ctcgaacatc ctcttcatct gcggtggggc tttcgccggt ctggacaagg
     2221 tcatcactga ccggtcggat aagtcgggca tcggattcgg tgctcaggtg aaaggcgagg
     2281 aggaccggaa gtccatcggc gaaacgctgc gccgtgtgga gccggaagat ctgatccgct
     2341 acgggcttat cccagagttc gtcggccgcc tgcccgtggt cgccacctta gaggaactgg
     2401 atacggacgc gctggtccaa atcctcacgg agccgaagaa cgccctgacc aagcagtaca
     2461 cgaagctgtt tgagatggaa ggcgtggaag tggacttccg tgaggacgca ctgcgcgcgg
     2521 ttgcccagaa ggccatggaa cgcaaaacgg gcgcccgggg cctgaggtcg attctggagt
     2581 cggtcctgct cgacaccatg tatgacctgc cttcttcgga ctcggtgacc aaggtggtca
     2641 tcgacgagag cgtcatcaaa ggcgaaagcg agccgatgct gatgtacgag aacgtcgaaa
     2701 aggcgcaggc cgctccgaag gagtgaagac aggcctgccc ggagcggcac catcctagcc
     2761 accggcgact acgctggtcg gcaacgccag ccatttatgg cgtatctgat tgcccacaac
     2821 tttcacagag ggctttttca tggtgacagt agctgatact acccgagtgc ccgtgcttcc
     2881 ccttcgcgac atggtggtct acccccatgg ggtgcaccct ctcttcgtgg gcacggaaag
     2941 ctccatcaag gcgctggacg ccgccatggc tgacgacaag cagatccttc tggttgccaa
     3001 gcgcgacgcc gaggtggaat cgcccggagc cgaagatctc tttgccgttg gaaccgtcgc
     3061 gacgatcctg cagttgctga agcttcccga cggcaccgtc aaggtcctcg ttgaaggagg
     3121 ggagcgggct cgcctgctcc agctcgatca ggaggaggac ttcctcgtcg cgagcgtcga
     3181 gctcgttgcc gaacccgttg tcgaagccag ggagtccgag gcgctggttc gaaccctgat
     3241 gagccagttc gagcagtacg tcaaggtcag caagaaggtg ccccaggaag tgctctcctc
     3301 gctgtccagc atcgacgacc cggcgagact tgccgacacc gttgccgcgc agatggcgct
     3361 gaagatcgac gacaagcagg ggattctcga ggcgttcgag ctcaaggagc ggatggacgc
     3421 cctgctagtg ctcatggact cggagatcga cacccagcag ctggagaagc gtattcgcgg
     3481 gcgcgtcaaa aagcagatgg aaaagagcca gcgcgagtac tacctcaacg agcagatgaa
     3541 ggccattcag aaggagctgg gcgaccttga agaagccccg aacgagttcg aggccctgga
     3601 ggcgcgaatc gaagagcagg ggatgtcgaa agaggctcgg gagaaagccc tggccgagct
     3661 cggcaagctg aagctgatgt cgccgatgtc ggccgaagcc acggtggtga ggagctatct
     3721 ggactggatg ctcagcattc cctggaaaaa acgctctcgc gttcgaaacg atctggcagc
     3781 ggcgcagcgc attctcgatg aggatcacta cggtctggag gaggtcaaag accggatcat
     3841 cgagtatctc gcggttcagt ccagggtccg caagatcaaa gggcccgtgc tctgccttgt
     3901 cggcccacct ggcgtgggca aaacgtccct gggcgaaagc attgcccgcg cgaccaaccg
     3961 gaagttcgtc cgcatggcgc tgggcggcgt ccgtgacgaa gccgagatac gcgggcaccg
     4021 gcgcacctac atcggttcgc tgcccggtaa gattctgcag aagatggcca aggctggtgt
     4081 tcgcaatcct ctgttcctgc tcgacgaggt cgacaagatg ggcatggacc ttcgcggcga
     4141 ccccgccagc gcgctgctcg aggtgctgga tcccgagcag aacaacagtt tcaacgatca
     4201 ctacctcgag gtggactacg atctatccga cgtgttcttc atctgcacct cgaactcgat
     4261 gaacataccg gccgccttgc tggaccgcat ggagatcatt cgtctgccgg ggtacacgga
     4321 agacgagaag ctcaacatcg ccaagcgcta tctcgtaccc aagcagatga agctcaacgg
     4381 cctttccgag gcagagctcg agatcggtga cggcgccatc gttcacctga tccgctacta
     4441 cacgagagaa gccggcgttc gcgggttgga gcgggaaatc gcgaagctcg ctcgcaaggt
     4501 ggtcaagcag cagtctcttg aaggcaaagg gagaggcaaa ggcaaaaagg gaacgatcac
     4561 cgtcgacgag ttgcagattg aagattacaa cggcgttcga aagttcagct acgggctggc
     4621 agagaccgag aaccagatcg gcatcgtcac cggtctcgcc tggacccagg tagggggcga
     4681 gctgctgaac atcgaggccg tggcggttcc cggaaaaggc cgccagacca gaaccgggtc
     4741 tcttggcgac gtgatgcagg agtccatcca ggcggcgctg accttcgtcc gcagccggtc
     4801 ggccattctc ggcgtcagga aggatttcca cgagaagttc gatctgcaca ttcacgttcc
     4861 cgaaggcgcg acccccaagg atggacccag tgccggcatc ggtatgtgca ccgcgctcgt
     4921 ttccgtgctg accggcatcc ccgtgcgcgc cgatgttgcc atgaccggcg aaattactct
     4981 gcgcggccag gtattgccca tcggcggcct caaggagaag ctgctggccg ctcatcgtgg
     5041 cggggtctaa cacgtgatca ttccggcgga gaatgagcgg gatctcaagg agatacccga
     5101 gaatgtcaaa gagaagctga ccattcatcc ggtgaagtgg atggacgacg ttctagagat
     5161 tgccctggaa cgcatgcccg agccacggct cgacgaagaa gagtcgagcg aaaagccgat
     5221 ctcggaggtc gcttcccagg atggcgagct gcccatcaat ccgcactgaa acccacgatt
     5281 taccgggctt tcaagcttgc aggcccgtaa ccgtgttggt ataaaaccgg cgtcgccacg
     5341 cctgaattgg cagcgtttct aggccatgct ggcgatagca aaccacatca ttgaggggcc
     5401 gattgtgaac aaaaccgaac taattgatcg tatcgccgag tccgccgaca tttccaaagc
     5461 cgccgccggc agggctctgg acgcagccat tgacgccatc accgactcgc tcaagcaggc
     5521 cgacccggtt tcgctggtgg gctttggaac gttcacagtg cgtgaccgcg ccgcgcgcac
     5581 gggcagaaac ccgcagacgg gtgcggagat ccagatcgct gccgcccgcg tacccgcctt
     5641 caagccaggc aaggcgttga aaggcgcact gaactagacc gtccaacact tgcccggaat
     5701 taaggcgcag cactgcgcct ttttttcttt tcgctgccga gacgcgtagg cgcgttaatc
     5761 gtcctctgaa ggagttcgta aatgctgcag aagctgcgtg atcagaccca gagcttcggc
     5821 ttcaaggtcc tggccggcgt gatgatcttc gtgctggccg tgtttggctt cggggcgttc
     5881 aacctgttcg tgaattccga tcccgaagtt gccagcgtga acggcgagga gatcacccag
     5941 agcgagctgc tggtagccat ggagcgcgag caggggagga ttgcggcgca gttcggtgaa
     6001 ggctttgacc ccagcctgat cgacggcgca cgtctacagg ccgccgtgct ggatcagctg
     6061 attgccaggc agctgctggg gcaggccgtg gacgagctcg gtttcggcgt gtcccagagt
     6121 cgcgtggacg aagcggtcac cgcgaacccc gcctttcagg tagacggccg attcgacgcc
     6181 gagctctaca agagacgggt acgtcagctg cgctacacgc cccagcagtt ccacagagaa
     6241 acgggtgccc tcatggctct ggaacagctt cggcgagggc ttaccgaaac ggctttcctg
     6301 acggactggg aagtgaggca gagcgctcgg ctgctgagtc agaagcggga cattgcttac
     6361 ctggcgttca ccgaagacga ctttctgcct ctcgtcgagg tttccgacga ggaggtcgca
     6421 acgcgctacg ccgagaacga gctggaatac cgcaccgagg agaccgtcga cgtcgagtat
     6481 ctcgagctca gtgcagaagg gctggtgaac gacgagtcca tctcggtgag cgaggccgcc
     6541 atcgaggaaa cctatgcggc ggattcggcg gaggctcttt ccggcgatcg ccgacaggcg
     6601 cgccacatcc tgctggaagt tggcgccagc cggacggcgg agcaggccga gaacgagctc
     6661 aacgcggtgc gagagcgaat cgtgggcggc gcggacttcg cgtccctggc cgaggagctc
     6721 agcgaggatc ctggatcggc cgcgctgggc ggcgatctcg ggatggtggc caagggcatt
     6781 ttcgatccgg cgttcgagca agcgctctgg gagctcgagg tcggcgagat ttccgagccg
     6841 gtcagaaccg actttggcta ccacctgatc cagctggacg ccgtcgacgt gcgggagctg
     6901 ccgccgctgt ccgagcaacg cgatgacatc gagctgcggc tgcggcggga acaggccgaa
     6961 gagctcttct ccgagcgtct gcgcgagttc gacaatctgg ctttcgagca acccgacggt
     7021 ctcgagggta tcgccgcggc cctggcgctg gagactcagt cggtggccgg cgtatcacgt
     7081 tcgcggggcg acggtgtttt cgccaacgcg tccttgaggg atgcggtatt cgccagcgag
     7141 gttctggaca acggctacaa cacacaggcg gtagcgctga cggacaaccg cgcggtggtc
     7201 gcccgcgtca aggcccgata cgagccggag gcgattcccc tggatgacgt tcgcgatgac
     7261 atacgcgcgg ccatcgcgtc ggaaaaggcg cgggttgcgc tcagacaagc ccagcagaac
     7321 gcgctggcgc gcgttcgttc cggcgagagc gtttccgcgg tggccgacga ctacggcctg
     7381 ggttggcaga ccttcgaact cgtcagccgc agctcggccg acgccccgcg agccgttctc
     7441 tcggcggcct tcagcctgcc acggcccggt gagcgggaga agagcgtggg tgaagccacg
     7501 ctcgatgacg gctccgttgc ggtggtcacc gttacccgtg tggtcgacgg ggatgtttcc
     7561 gtgatggcgg aaagagagat cgaaagcatg cgcggcttcc tgggaaatcg cgtggcttct
     7621 ctggatttcg cggggctgac gaccactctg gaaagggaag cgtcgatcag cacgccgtag
     7681 gtcgaccccg tccggcgacc ggctggtcag ttactgtcca gccgctacgt tgacgtgcag
     7741 tttcagcgac tggccgggct gcaggtagtt gtccacgtcc agctggttcc actgcgcgat
     7801 ctcgctgacc cgcacgttga agcgagcggc gatgcgcgcc agcgagtcgc ccttgcgcac
     7861 gccgtagcgc accgtgcgaa ttacttcccg tccgtcttct cccgcagcgg gcgtcgagcg
     7921 ctgagcgccg ggaatggtga gagtctggcc taccttcagc aggtccttgg gccccacgtg
     7981 attggctttc atgatgcgcg tcatcgaaac cccgtgcgcc cgactgatgg tccacaggga
     8041 atctccgggc tgcaccgcgt attccagtcc tttctgagcg cggctgcggg gactgcggct
     8101 gggggccgaa tccgatttgg gaatgtacat agcctggccg gcacgaattg aggagcccgt
     8161 cagattgttc acccgtcgca aggtggccgt gtccgttcgg tgacggtggg cgatttcgct
     8221 gagggtgtct ccgtctgcca ccttgatccg cagccactgg acccgctcgc cagcgggaac
     8281 ggccgcaagg gccgcctggc tcagtgctgg ttcaagtgcg ggaatcatca gccggtgagg
     8341 accatccggc ggcgtcgacc actggttcag ggccggattc cagtcgtaga gcgcgtcgag
     8401 attcatggaa agggtgtcgg ctgccttggc gaggtcgaac tgtccctgcg tgtcgacggc
     8461 cacgaacggc acgtccggcg aaagctcggg cagcgtcagg ccgtaagcct cggggtcggt
     8521 taccaccgct gccaacgcca gcaggcgcgg cacgtacatc tgggtttccc ggggcagagc
     8581 catctcccag aaggtggccc ccggtgcggc tttccgctga gcgcggcgga cgttgccctc
     8641 tccagcgttg tagcccgcga cggcaacatg ccagtcctcg aaaagcagat gaagatattc
     8701 gaggtagtcg agggcggctt gagtagccgc gatgggatcc cgacggccgt cgtaccacca
     8761 gtttcgctgc agcccgaatc gcttcgccgt ggcggggatg aactgccaca gaccggcggc
     8821 gccgccgtga gaaaacgcat acgggtcgag cgcgctctcc acgatgggga gcagggcgag
     8881 ctcaccggga agctcccgag cggccacctc cccgtgaatg taggcgagga accgctccat
     8941 tcggggtttc agattgtgca ggtagcgcgg attctcgcgc agccagcgca gctcctgctg
     9001 cacgcgtttc tggtcgaggc tgtgggccag ctcaaagcgc tcgcggatgt cgcgccacag
     9061 atcgggtttc agctcgtagg tgagcgtcgg ctcgatgacg ggaggcggaa ccgcgacggg
     9121 cgggggaggc tcggcggccg cggtttcggc cgcttcgggg atgggattgg tctggcaccc
     9181 cacgagcgac gccaggatca caaacatcag cgtgcgcagg gtcgaggcaa accgaacgac
     9241 aggtgcggtt gcggtcatcg agtggtgcct tcagaatgat ccttcaactt tgcggtatcc
     9301 ctgtgcgatt ggcaagatcc ttgcgtcatc agatgtccgc ctggctggct acaccggtcg
     9361 gtgagcgcct ccttgccgac gaggcgccct acgtggcgga aacggcccgc cgctttcacg
     9421 gcgatacgct gctctgggca gggtgccata cccccctgac tgataccgtg cgcggatgca
     9481 tggttcgcaa tcgcgtgttc gcgctcgatg gttcgagccc ggcgggcgag ggcctcacga
     9541 cctttcgctg cgagctggac gccatacctc tggccaacaa cgccctggat ggtgtgctgc
     9601 ttcaccatgc gctggagatg gccgaggacc cgcgcaccgc gctgcgggag ctctcccgcg
     9661 tgcttgcggc cggcgggcgg ctgacggttg tggggttcaa cccgttcagt ttctggggcg
     9721 ttcggatcgc ttacgcgcga gtctttcgcg acagcttcag cgggctgcgg ctggtcagcc
     9781 ccgctcggct tgtggactgg ctgaccgtgc tcggtttcga gctttgcgag gaagtgcgct
     9841 ttaccgccta caacctgccg ttcagattgg gaaataccgg cgagcaggac gaacggtcga
     9901 cgcagcagct gattccgtcg agttggggtc cccggctgcg aaacgcgttg gatcacctca
     9961 accccccggt cggcggggtt tacatggtca gcgcggtcaa gcaggcgctc ggcgttcgcc
    10021 gggactggcg tctgtctgct gcccgggaac cgaaactcgc ccctgtggcg tatccgaaac
    10081 tgagcgcgcg gaaccggatt gagcgctccc gttgagatct tcaccgacgg ggcctgcagg
    10141 ggcaatccgg ggcctggcgg ctgggccgct ctgctgcggt atggggatgc cgagaagctc
    10201 atcagcggtg ctgctccgga cacgaccaac aaccgaatgg agctgcaggc ggcgatctcc
    10261 gcgctcgagg tgctcacgcg gccgagcagc gtggccatta ccaccgactc tgaatatctc
    10321 aggcagggca tcaaccagtg gattcagaaa tggaagcgca acggctggcg aacgtctcaa
    10381 cgaaagccgg tcaagaaccg cgatctctgg caaaagctgg atgagctggt gcagcagcac
    10441 cagatcgagt ggcattgggt acgcgggcac agcgggcatc cggaaaacga acaggtggac
    10501 gaagcggcga acgtggccat cgaccgcatg ctcacaggag gcgaatcttg acgcggcaga
    10561 tcgttctcga cactgagacc accggcctcg aagctcagaa ggggcatcgg gtcatcgaga
    10621 tcggtgccgt ggaaatgatc gaccgcaagc cgaccggccg tcacttccac aagtacatca
    10681 accccgatcg cgatattgat gacggcgcct tcgaggtgca cggtctgagc tcggagtttc
    10741 tcagcgataa gccccggttc gaggacatcg cgcaggagtt tctcgctttt gtttcgggtg
    10801 cggagctcgt catccacaat gcgggtttcg acgtcggatt tctgaactac gagctggcaa
    10861 tgctcgaagc ggttccggac ggaatcgaga gcgtttgcct cgtaacggac tcgctttccc
    10921 tcgcccggtc caagcatccg ggccagaaaa acaatctgga tgcgctctgt cgccggtacg
    10981 gggtggacaa ctccgcccgc acgctccacg gggcgctgct ggacgcggaa atccttgccg
    11041 acgtttatct agccatgacg ggcgggcaga cggctctgtt cggctcggag gacgccgtgg
    11101 gggccggcgt cgatcgggat caggcggtcg cgtcctttga cggccagcgt ccacggttga
    11161 aggtcatatt ggctaccgac gaagagatcc aggctcacga agccgtgctg gacttcctcg
    11221 agcagaaatc tggcgattgc gtctggcgcc agccatccgg gtcctgatca gagcaggtac
    11281 tgcaccccga accagcccgt gatccccagg acgatggtgt aggggagcgc catgaaaacc
    11341 atcttgctgt acgacaaccg caccaggggg gcgatggacg acgtcagcag gaacagaaag
    11401 gcggcctggc cgttgggggt cgctacgctc ggcaggttgg tgccggtgtt gatcgcgatc
    11461 gccaggtttt cgaagtgctc gcggccgatc tggccttcct cgaaagcgtt cgtcacctcg
    11521 ttgatgtaga cggtcgcgac gaagacgttg tcgctgatgg cggatagcac gccgttggcg
    11581 atgtagaaca tgccgggctg cacggacacc tctagggtga ggacgtagtc gatcaccggg
    11641 gcgaacaggt gctggtcgtg gatgacggcg actatcccga agaacacgac gagcagagac
    11701 gtgaaaggca gcgcctcctc gaaggcgcgg ccgagattgt gctcctcgat gacgccgttg
    11761 aaagcagtct ggatcacgat gaccatcagc ccgatcagcc cgacctcggc ccaatgcatg
    11821 gccagcgcga cgacgaggag cattgcggcc accgcctgaa tcactaagcg ggccttgtcg
    11881 cggccgctgc gtgcttcagc ttcggcctgg tcgaagcgct cgagaatgtc gcgcaccggg
    11941 ccgggcaggt cggttccgta gccgaaagct ctggcttttt ctaacacgac gcaggtagtc
    12001 agcccgacca ccagaaccgg catcgtgacg ggtgccatgc gcaggaagaa ctccatgaac
    12061 tcccatccca cctggccggc gatcagcagg ttctgtggtt cgcccaccgt ggtgcagaca
    12121 ccgcccaatg cggttc
//
LOCUS       DBNHLM010000341         3344 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_231298, whole genome shotgun sequence.
ACCESSION   DBNHLM010000341 DBNHLM010000000
VERSION     DBNHLM010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_231298"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..141
                     /locus_tag="AC16QF_15765"
     CDS             7..141
                     /locus_tag="AC16QF_15765"
                     /inference="COORDINATES: protein motif:HMM:NF051921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7931 domain-containing protein"
                     /protein_id="HIR6450066.1"
                     /translation="MPDYREYDGLTNLYDPVQASKLTERFDYLWNLSSPDPELRILRI
                     "
     gene            281..1585
                     /gene="aceA"
                     /locus_tag="AC16QF_15770"
     CDS             281..1585
                     /gene="aceA"
                     /locus_tag="AC16QF_15770"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019361792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HIR6450067.1"
                     /translation="MALHFIPRKPREEQVRELQTEWDTNPRWKDVKRGYTAADVVNLR
                     GTIPHRSMLAAHGADKLWDYLQNEDFINSLGAVTGGQAVQQVKAGIKAIYCSGWQVAA
                     DANDSETMYPDQSLYAVNSVPTLVNRINNAFRRADEIQWSKGIDDGIDYFAPIIADAE
                     AGFGGILNAFELMKSMIGAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLIAAR
                     LAADVCDVPTILLARTDANAADMVTSDIDPWDKDFLTGERTPEGFYKTHAGLDQAISR
                     GLAYAPYADLLWCETAVPDLDEAKRFAEAIKKEYPDQMLSYNCSPSFNWRANLDDATI
                     AKFQKELAAMGYKYQFITLAGIHSMWFNMFNLAQGYASRQMSAYVELQEEEFAAADRG
                     YTFVSHQQEVGTGYFDQVATVIQGGQSSVTALTGSTEEAQFH"
     gene            complement(1655..2251)
                     /gene="thrH"
                     /locus_tag="AC16QF_15775"
     CDS             complement(1655..2251)
                     /gene="thrH"
                     /locus_tag="AC16QF_15775"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019689458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HIR6450068.1"
                     /translation="MDVLCLDLEGVLIPEIWQAVADRTGIEELRKTTRDIPVYDDLMG
                     MRLEVLGANDLSMSLIESVIDGLEPLPGASDFLGWARARYQVAIISDTFYQFANPLMA
                     KLGWPTLLCHNLVVENDRIVGYRIRQPDPKRCSVQAFKSLNYRVLAAGDSYNDIAMLE
                     EADAAFFYRAPENVLAEYPQYPCAGSYGELQALLKAAA"
     gene            2363..3076
                     /locus_tag="AC16QF_15780"
     CDS             2363..3076
                     /locus_tag="AC16QF_15780"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HIR6450069.1"
                     /translation="MQPSPAPDQRPQEVLADAVGRFAPGHIAISFSGAEDVVLIDMAH
                     QLGLAVDVFSLDTGRLHAETYRFIESVRNRYGVAIEMVMPEPTAVGELVKSKGLFSFY
                     EDGHSECCGVRKIKPLKRKLSTLDAWITGQRQDQSVTRTSVPTEQEDSAFAGRSGSLV
                     KFNPLAQWSSADVWAYIREHGVPYNPLHDRGFVSIGCEPCTRPVGPHEHERSGRWWWE
                     EATRKECGLHAQNVVRIVG"
     gene            complement(3099..>3344)
                     /gene="cysB"
                     /locus_tag="AC16QF_15785"
     CDS             complement(3099..>3344)
                     /gene="cysB"
                     /locus_tag="AC16QF_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715759.1"
                     /note="LysR-type transcriptional regulator; contains
                     helix-turn-helix (HTH) motif; in Escherichia coli this
                     protein regulates cysteine biosynthesis by controlling
                     expression of the cys regulon; autoregulates expression;
                     crystal structure of Klebsiella aerogenes showed tetramer
                     formation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HIR6450070.1"
                     /translation="ATMALDEELDDDLVAIDAAHLFSPSVTHIGFRRGTFLRRYMYDF
                     IERFAPHLDQQMVDLAAACATSAERAALFEDLELPVR"
BASE COUNT          671 a         1088 c         1019 g          566 t
ORIGIN      
        1 tggctgatgc ccgactaccg ggagtatgac ggtctgacca acctctacga tcccgtccag
       61 gcatcaaagc tcaccgagcg gttcgactac ctgtggaacc tcagcagccc cgatccggag
      121 ctgcgtatcc tgcgtatctg agacgggcaa ccggcgccgt cgttcacgtc gctgatatat
      181 tcccattggg aaagcattaa ttgttttcct ttcgctattc ttttcaatct gcgtctctac
      241 aatcccggcc ccgacattcc ttgagacggg cgaacgatcc atggcgctac atttcattcc
      301 gcggaagccc cgcgaagagc aggtcagaga attgcagacg gagtgggaca ccaatccgcg
      361 ctggaaagac gtcaagcgcg gttacacggc ggccgacgtc gtcaatctga ggggcaccat
      421 accccaccgc agcatgctgg ccgctcacgg cgcggacaag ctctgggact acctgcagaa
      481 cgaggacttc atcaactctc tcggcgccgt caccggcggc caggccgttc agcaggtcaa
      541 ggcgggcatc aaggccatct actgttcagg ctggcaggtg gccgcggacg ccaacgactc
      601 ggagaccatg tacccggacc aatccctcta cgcggtcaac tccgttccta ccctggtcaa
      661 ccggatcaac aacgcgttcc ggcgggccga cgagattcaa tggtcgaaag gaatcgacga
      721 cggcatcgac tacttcgcac ccatcatcgc cgatgccgag gcgggcttcg gcggcatcct
      781 caacgccttc gagctcatga aatccatgat tggcgccggg gctgccggcg tgcacttcga
      841 ggaccagctg gcctcggtga agaagtgcgg ccacatgggc ggcaaggtgc tggtacccac
      901 ccaggaggcg gtgcagaagc tgatcgccgc acgtctggcc gccgacgtgt gcgacgtgcc
      961 gaccatactg ctcgcgcgca cggacgcaaa cgccgccgac atggtaacca gcgacatcga
     1021 cccctgggac aaggattttc tgacaggaga gcgcacgccg gaagggttct acaagaccca
     1081 cgcgggcctt gatcaggcca tctctcgcgg cctggcctac gcgccgtacg ccgatctgct
     1141 gtggtgcgaa accgcggtac cggatctgga cgaggccaag cggttcgccg aggcgataaa
     1201 gaaggaatac cccgaccaga tgttgtcgta caactgctcg ccgtccttca actggagggc
     1261 caacctggac gacgcgacca tcgccaagtt ccagaaagag ctggcggcca tgggttacaa
     1321 gtatcagttc atcacgctgg caggcattca cagcatgtgg ttcaacatgt tcaacctggc
     1381 gcaggggtat gcgagcaggc agatgtccgc ctacgtggag ctccaggagg aagagtttgc
     1441 cgccgccgac agggggtaca cgttcgtcag ccaccagcag gaagtcggca ccggctactt
     1501 cgaccaggtc gccaccgtca tccagggcgg gcagagctcg gtgaccgctc tcaccggttc
     1561 aacggaagaa gctcagttcc actgacacac ggccgtcgct gccgggcgca atgcccggcg
     1621 gcgcagcgcg ccacccgttg aagcgatcgg gccgtcaggc cgcagccttt aaaagcgcct
     1681 gcagctcccc gtaagacccc gcgcacgggt actgcggata ctccgccagc acgttctccg
     1741 gggcccgata aaagaacgcc gcgtctgctt cctccagcat ggcgatgtcg ttgtaggagt
     1801 cccccgcggc cagcacccgg tagttgagag acttgaaagc ctgaaccgag catcgcttgg
     1861 gatccggctg gcgaatccgg tacccgacga ttcgatcgtt ctctaccacc aggttgtgac
     1921 acagcagcgt cggccagccg agcttggcca tcagcgggtt ggcgaactgg taaaacgtgt
     1981 ccgagatgat ggccacctgg tagcgcgccc tggcccaccc cagaaagtct gaagcgcccg
     2041 gcagcggctc caggccgtcg atgacggatt cgatgaggct catggacagg tcgttcgcgc
     2101 cgagcacctc gagccgcatg cccatgagat cgtcgtaaac gggaatgtcc cgtgtggtct
     2161 ttcggagctc ctcgatgccg gtgcggtctg cgaccgcctg ccatatttcg gggatcagca
     2221 ccccttccag atccaaacac aaaacgtcca cgggttgggt tttccagctc gattgagggc
     2281 gtaagcatat accaagagat tattttctct gccgcgcgag cattgctagt ctgcgcgcca
     2341 actcccggac acgaccagga ccatgcagcc aagccccgcg ccagatcaaa gaccccagga
     2401 agtgctcgcc gatgcggtcg gccgttttgc tcccggccac atcgccatct cgttcagcgg
     2461 cgccgaggac gtcgtgctga tcgacatggc ccatcagctg gggttggccg tggacgtctt
     2521 cagcctggat accggccgtc tccacgccga aacctatcgc ttcatcgaat ccgtgcgcaa
     2581 ccgctacggc gtggccatcg agatggtcat gcccgaaccc acggcggtgg gcgagctggt
     2641 gaagagcaaa ggactcttca gcttctacga ggacggccac tccgaatgct gcggcgttcg
     2701 caagatcaag ccgctgaagc gcaagctgtc cacgctggat gcctggatca ccggtcagcg
     2761 ccaggaccag agcgtcacga gaaccagcgt ccccaccgag caggaagatt ccgcctttgc
     2821 cggcagaagc ggttctctgg tcaagttcaa cccgctggcg cagtggtcgt cggcggacgt
     2881 ctgggcatac atccgcgagc acggcgtccc ttacaacccc ctgcacgacc gcggcttcgt
     2941 ctccatcggc tgcgagccgt gcacgcggcc ggtggggccc cacgagcacg agcgctccgg
     3001 acggtggtgg tgggaagaag ccacccgcaa ggagtgcggc ctgcacgccc agaacgtcgt
     3061 caggatcgtc ggctgacccg cggccaggcc ctcgagcgtc agcgcacggg aagctcgaga
     3121 tcctcgaaca gcgcggcccg ctccgcggag gtcgcgcagg cggccgcaag gtcgaccatc
     3181 tgctgatcca ggtgcggcgc aaaccgctcg atgaagtcgt acatgtagcg ccgcagaaac
     3241 gtaccccgac gaaagccgat gtgggtgacg ctgggtgaga acaggtgggc cgcgtcgatg
     3301 gccaccaggt catcgtccaa ctcctcgtcg agcgccatgg tggc
//
LOCUS       DBNHLM010000342         2225 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_469974, whole genome shotgun sequence.
ACCESSION   DBNHLM010000342 DBNHLM010000000
VERSION     DBNHLM010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_469974"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="AC16QF_15790"
     CDS             complement(<1..553)
                     /locus_tag="AC16QF_15790"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR6450071.1"
                     /translation="MTLLASSIARLDADARWALRGHELFFASLAVGEAQRAEGAAAEL
                     LASVEHGARALGVGKIGIDRPQDEALVAWYQQRGYYPGAYPRGYLGEGRGEGPALLAK
                     DLNAAREMEVFFRGWAPELIGTLLFVIDGSRVLLIDKKTGHGAGKINAPGGKLEPGES
                     PVACAVREVREEIGITVAAPRLHA"
     gene            656..1165
                     /locus_tag="AC16QF_15795"
     CDS             656..1165
                     /locus_tag="AC16QF_15795"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HIR6450072.1"
                     /translation="MTGIEVVEGDITTQSVDAIVNAANESLLGGGGVDGAIHRAAGPG
                     LKAECRRLGGCPTGEARITGGYDLPAAHVIHTVGPIWRGGDAGEAALLTACYRNSLDL
                     ARRHHIRSIAFPAISTGAFGYPLAEATAIAVATVGSFIATTPLLARFVCFDARTARAY
                     REELAAQTG"
     gene            complement(1153..1779)
                     /locus_tag="AC16QF_15800"
     CDS             complement(1153..1779)
                     /locus_tag="AC16QF_15800"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF020341.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR6450073.1"
                     /translation="MDRARALAMIQTMAEMQESHNRHVHPEWIEQGYAYYRAVWVECA
                     ELLDHFGWKWWKKQQPDLGQVKLEVVDIWHFGLSDLIRAGTLDTAAPVLLEVSASEPT
                     DLEGFRLAVEALAADTLSSRAFAMAPFAAVLESLPMSFAELFEMYVGKNVLNNFRQDH
                     GYKTGEYQKLWQGREDNEHLIDILGELDCPAESVPAELYRALQARYPV"
     gene            1840..>2225
                     /locus_tag="AC16QF_15805"
     CDS             1840..>2225
                     /locus_tag="AC16QF_15805"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR6450074.1"
                     /translation="MAKSPSTALVMHPVVRDELITHAHMERLRKTVNFIDEEPFRDLD
                     AIGGALGQVELLITSWGCPRVDADCLARLPRLKLIAHLAGSVKGFLDDIVWRRGILVT
                     NAVAANAVPVAEYTLAAILFANKRVF"
BASE COUNT          385 a          780 c          684 g          376 t
ORIGIN      
        1 cggcgtggag ccgcggcgct gcaaccgtga tgccgatctc ttcgcgaact tcgcggactg
       61 cgcaggccac agggctttct cccggctcca gcttgccgcc cggcgcgttg atcttgcccg
      121 ccccgtgacc cgttttctta tcgatgagca gcacccgcga gccatcgatg acgaagagca
      181 gggtcccgat gagctcaggc gcccacccgc gaaagaagac ctccatctca cgggcagcgt
      241 tcagatcctt tgctaggaga gcggggcctt cgccccgacc ctccccgagg tacccccgtg
      301 ggtaggcccc tgggtagtag ccgcgctgct gataccaggc gacgagcgcc tcgtcttgcg
      361 gccggtcgat tcctatcttg cccacaccca gagcccgagc accgtgctcc accgaagcca
      421 gcagctcggc ggcagcgcct tccgcccgct gcgcctcgcc gaccgccaac gaggcgaaga
      481 agagctcgtg gccgcgcagc gcccaacggg cgtccgcgtc gagccgtgcg atggagctgg
      541 ccagaagcgt catggcccgc cattgtcgac aggccgccgg aagatggcaa ttttacccgg
      601 ggacgggtgg gggctaccct tcgggcgaag ggcgacagct tcgggcggca tcgacatgac
      661 cgggatagag gtcgtggaag gcgacatcac cactcagtcc gttgacgcga tcgtcaacgc
      721 ggccaacgag tctctgctcg gcgggggcgg cgttgacggt gccattcacc gggccgccgg
      781 ccccggtctg aaggcggaat gccgcaggct cggcggatgc cccaccggag aagcacgaat
      841 cacgggaggc tacgacttgc ccgccgccca cgtcattcac acggtagggc ccatctggcg
      901 cgggggtgac gccggggagg cggcgctgct gaccgcctgc taccgaaatt ccctggatct
      961 ggccaggcgc caccacatcc gcagcatcgc cttcccggcc atcagcaccg gcgcgttcgg
     1021 ctacccgctg gcggaagcca ccgccatcgc cgtcgccacg gtcggcagct tcatcgccac
     1081 cacacccctg cttgcccggt tcgtctgctt cgacgcccga acggctcgcg cctaccgcga
     1141 ggagctcgcc gctcagacgg ggtagcgggc ttgcagagct cgatagagct cagcaggaac
     1201 cgactccgcg gggcaatcca gctcacccag aatgtcgatc aggtgctcgt tgtcctcccg
     1261 gccctgccag agtttttgat actccccggt cttgtagccg tgatcctgcc ggaagttgtt
     1321 gagcacgttc ttgccgacgt acatctcgaa aagctccgcg aaggacatcg gcaggctttc
     1381 gagcaccgcg gcaaacggcg ccatggcgaa agcacggctg gaaagcgtgt cggccgcgag
     1441 cgcctccacc gccaggcgga acccctcgag atccgtgggc tcgctcgccg atacttccag
     1501 cagcaccggc gctgccgtat ccagggtgcc cgcccgaatc agatcgctca ggccgaagtg
     1561 ccagatgtcc accacctcga gtttcacctg gcccagatcg ggctgctgct tcttccacca
     1621 tttccatccg aagtggtcga gcagctcggc gcactccacc caaacggccc ggtaataggc
     1681 ataaccctgc tcgatccatt ccgggtgcac atggcgattg tggctctcct gcatctccgc
     1741 catggtctgg atcatcgcca gcgcgcgcgc tctgtccatt ttcaaaccga tcctcgcggg
     1801 taaacgaaag acagaaaatt atggggtatt cttcacccta tggccaaaag cccgagcacc
     1861 gcgctggtta tgcatcccgt cgtccgcgac gagctgatca cgcacgctca catggagcgg
     1921 ttgcgcaaga cggtcaactt catcgacgag gaaccgttcc gcgacctgga cgccattggg
     1981 ggcgcgctcg gccaggtaga gctcctgatc accagctggg gctgcccccg ggtcgatgcg
     2041 gactgcctcg cacggcttcc acgcctgaag ctgatcgccc atctcgccgg cagcgtgaag
     2101 ggctttctcg acgacatcgt ctggcgccgc ggcattctgg tgaccaatgc ggtggcagcc
     2161 aatgcggtgc cggtggccga gtacaccctg gccgccatcc tcttcgcgaa caagcgggtg
     2221 tttca
//
LOCUS       DBNHLM010000343         3914 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1249412, whole genome shotgun sequence.
ACCESSION   DBNHLM010000343 DBNHLM010000000
VERSION     DBNHLM010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1249412"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="AC16QF_15810"
     CDS             <1..734
                     /locus_tag="AC16QF_15810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6450075.1"
                     /translation="LDQISGGRAVLGVGLGYRPYEYEGFGLDFHTRGKRLDELLTAIR
                     EGWTRGEFRHDGEHFRIPPSPVNPPCVQKPQVPILVGGSAPAAITRAARLGDGWFALP
                     QEGLEIMRRQVDAYRAACREAGRTPYVCLMRNAWIAPTMEQVQAEWLPRMVEFHKTFA
                     DARADRSDTVIERLLAGETFTLDEYIRARAIAGTPEVCIAEIERWQAAIQPDEYSLIF
                     GGSDDAARLEDAVTLFAREVMPAFS"
     gene            749..1168
                     /locus_tag="AC16QF_15815"
     CDS             749..1168
                     /locus_tag="AC16QF_15815"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6450076.1"
                     /translation="MAASLEDNPMPVNLIDYMPFARVLGLEVTHSDKDKVTASIEVSE
                     DLCTTGKIMHGGAVMAVADTLGAIGAYLNLPDGAKATTTIESKTNFLGAARLGTRVLA
                     ETTPVHVGGRTSVWQTRLTGEDGKPVALVTQTQLVLS"
     gene            1260..3182
                     /locus_tag="AC16QF_15820"
     CDS             1260..3182
                     /locus_tag="AC16QF_15820"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HIR6450077.1"
                     /translation="MADIFISYARSTRRQAEAIGEALVELGYSIWRDDALPAHRAYAD
                     EIQKQLNIAKAVVVVWSADAIKSDWVRSEADRARNDRKLVQLTVDGAALPMPFDQIQC
                     ANLAGWRGEGRAAGWATVLESIRALTDTAGKESPVSAGTQSQQQPEPILAVLPFDNLS
                     TDPEMQFFSDGVSEEIIQRLSRGASLKVIGRTSSFQFRGERKAEAASALGCSHILDGS
                     VRRGAGRMRLSAHLVETSSGTTLWSERYDRSLEDVFAVQDDVSENIAGALHHTFQSFS
                     TPAVDPGAYDLYLRASPESYAPEEMRTNIGLLEVATQRAPDFPEAWGRLAYLRAFLRF
                     YEPFPGRGASAAIIARESAHALQADPENGDALAARAFVVAPFGNFIELDTAVERLRSA
                     PGDFDARKFAGWLLRNFGRVRESLAEDERAYLGNALDHMCANTLALARLAAGRVAEAL
                     PVLENLVERVPDMVFPFANLLRAQALLENWEALDELLELNADRPLREFQDTLDFIRTK
                     RNPSAENIAVWKSQFEAHIARTGCIDISRVVYAAHLGLVDEAYQATEQAHLGPTGTNE
                     DVMGPDGYRTSLLFQAAMPELRNDPRFVPLCARLGLVEFWTSTGKWPDCADEVPYDFR
                     AACEDARGIPKLEFGF"
     gene            complement(3227..>3914)
                     /locus_tag="AC16QF_15825"
     CDS             complement(3227..>3914)
                     /locus_tag="AC16QF_15825"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6450078.1"
                     /translation="ATHPQISDYYDDDYRNTDFELYETGHNLMKMKGSLPSLRYDEPF
                     AINETLAADMQNWGLDPAAFEGRAHAVREALQAQRRKLGPARGYRYFERLPASWLTDA
                     YHFNLFPGTSLTVVADGAQMQRCEPHPTDPTRSIYEHWFLALKGADSDLVPGPEGMVP
                     WEEAERRVVEYGQETLSEVADQDLERASGQQLGVQSRGFAGAYLSGQENRVQQFHNFI
                     DRYLRDDIGS"
BASE COUNT          643 a         1342 c         1282 g          647 t
ORIGIN      
        1 tgctggacca gatatccggc gggcgcgcgg tgctgggggt cggcctcggc taccggccct
       61 acgagtacga gggcttcggg ctcgatttcc acacccgcgg aaagcggctg gacgagctgc
      121 tcaccgcgat cagagaagga tggacgcggg gcgaatttcg ccatgacggc gagcacttcc
      181 gcataccgcc gtcgccggtg aacccgccct gcgtgcagaa gccccaggtg ccgattctgg
      241 tgggtggctc ggcacccgcc gccatcaccc gggccgcccg tctgggagac ggctggttcg
      301 ccctgcccca ggaaggcctg gagatcatgc ggcgccaggt ggacgcctac cgcgcagcgt
      361 gtcgggaagc cgggcgcaca ccctacgtct gcctgatgcg caacgcctgg atcgcgccca
      421 ccatggaaca ggttcaggcc gagtggctgc cgcgcatggt cgagtttcac aaaaccttcg
      481 ccgatgcccg ggccgatcgg tccgacaccg tcatcgagcg cctgctggcc ggggagacgt
      541 tcacgctgga cgagtacatc cgagcgcgcg ccatcgccgg cacgccggag gtctgcatcg
      601 ccgagatcga gcgctggcag gcggccatcc agcccgacga gtattcgctc atcttcggcg
      661 gctcggacga cgccgcgcgg ctcgaagacg cggtgaccct gttcgcccgc gaggtcatgc
      721 cggcgtttag ctgaacacgg acgcccggat ggcggcgtca ttagaggaca accccatgcc
      781 cgtgaacctg atcgactaca tgcccttcgc ccgcgtgctg gggctcgagg tgacccattc
      841 ggacaaagac aaggtgaccg catccatcga ggtgagcgag gatctgtgca ccaccggcaa
      901 gatcatgcac ggcggggccg tgatggcggt ggcggacacc ctcggcgcca tcggcgccta
      961 cctgaacctg cccgacggcg ccaaggccac caccaccatc gagagcaaga ccaactttct
     1021 aggtgcggcg cgtctgggca cccgagtcct ggcggagacg acgcccgtcc acgtgggcgg
     1081 gcgcacctcc gtgtggcaga cgcgcctcac cggcgaggac ggcaagcccg tcgccctcgt
     1141 cacccagacg cagctggttt tgagctgacc ggctgacggc ggcagtactt gggtctgccc
     1201 aaccggtttc tggtttcccg ttatactcgc cgtcgctcct gtcaccaggt cgagcgttag
     1261 tggccgacat cttcatctcg tacgcgcggt cgacccggcg ccaggcagag gccattggcg
     1321 aggccctggt agagctcggt tactccatct ggcgagacga cgccctgccc gcccatcgcg
     1381 cctacgccga cgagatccaa aagcagctca acattgccaa ggcggtggta gtcgtctggt
     1441 ccgcggatgc gatcaagtcg gattgggtgc gctccgaagc cgaccgtgcg cgcaacgacc
     1501 gcaagctcgt ccagctgacc gtcgacgggg cagcgctgcc catgccgttc gatcagattc
     1561 agtgcgccaa cctcgccggc tggaggggag aaggccgggc cgccggctgg gcgacggtgc
     1621 tggagagcat tcgtgcgctc accgatacag ccgggaaaga atcaccggtg tctgcgggca
     1681 cgcaatcgca gcagcagccg gagcccatcc tcgccgttct gcccttcgac aacctctcca
     1741 ccgatccgga gatgcagttt ttctccgacg gggtcagcga ggagatcatt cagcggctgt
     1801 cccggggggc gagcctcaag gtcatcggcc ggacgtcgag ctttcagttt cgcggggagc
     1861 gcaaggcgga agccgcctct gcactcggct gcagccacat cctggacggt tcagttcgac
     1921 gcggcgcggg gcggatgcgc ctgagcgcgc acctcgtgga gacgtcctcg ggcaccacgt
     1981 tgtggtcgga gcgctacgac cgttccctgg aggacgtgtt cgccgtccag gacgacgttt
     2041 ccgagaacat cgccggggcg ctgcatcaca cgtttcagag tttctcgacc cccgccgtgg
     2101 acccaggcgc ctacgacctt taccttcggg cgagccccga gtcctacgcg cccgaggaaa
     2161 tgcgcacgaa catcggcctg cttgaagttg ccacccagcg tgcgcccgat tttcccgagg
     2221 cctggggccg gctcgcctac ctgcgagcct tcctgcggtt ctacgaaccg tttcccgggc
     2281 gcggcgccag cgccgcgatc atcgccagag aatcggccca tgctttacag gcggatcccg
     2341 agaacggcga cgccctggcc gcgagggcat tcgtggtcgc gcctttcgga aacttcatcg
     2401 agctcgacac tgccgtcgag cgcctgcgca gcgcgcccgg agacttcgac gcgagaaagt
     2461 tcgccggttg gctgctgagg aacttcggcc gggttcgcga aagcctcgcc gaggacgagc
     2521 gcgcctacct cggcaacgcc ctcgaccaca tgtgcgccaa tactctggct ctggcccgac
     2581 tggcagcggg acgcgtcgcg gaagcgctgc cggtcctgga gaatctggtc gagcgcgttc
     2641 ccgacatggt ttttcctttc gccaacctgt tgcgtgccca ggccctgctc gagaactggg
     2701 aggcgctgga cgagctgctc gagctcaatg ccgaccgtcc cttgcgggaa tttcaggaca
     2761 ctctggactt catacgcacc aagcggaacc cgtcggcgga gaacatcgcc gtctggaaga
     2821 gccagttcga ggcgcacatc gcgaggacag gctgcatcga catctcccgc gtcgtctacg
     2881 ctgcccatct cggcctggtc gacgaagcct accaggccac cgagcaggcc cacctgggac
     2941 ccaccggcac gaacgaagac gtcatgggcc cggacggcta ccgcacgtcg ctgctgtttc
     3001 aggcggccat gcccgagctg cgcaacgacc cgcgcttcgt acccctgtgc gcccggctgg
     3061 ggctggtgga gttctggaca tccaccggca agtggccgga ttgcgccgac gaggtgccct
     3121 acgacttccg cgccgcctgc gaggatgcgc gcggcatccc caagctggag ttcggtttct
     3181 gagacgcagg cccggacgcg cgggactgcc agcgcagcgg gtgccgctag gaaccgatat
     3241 cgtcgcgcag atagcggtcg atgaagttgt ggaactgctg cacccgattc tcctgacctg
     3301 agagatacgc gcccgcaaat cccctcgact gcactccgag ctgctggcct gaggcgcgct
     3361 cgagatcctg atcggccacc tcgctcaacg tctcctggcc gtactccacc acccgccgct
     3421 cggcctcctc ccagggcacc atcccttccg gcccgggcac caggtccgag tctgcgccct
     3481 tcagcgccag gaaccagtgc tcgtagatgc tccgtgtggg gtccgtgggg tgcggctcgc
     3541 agcgctgcat ctgggccccg tccgccacca cggtgagcga ggtgccggga aagaggttga
     3601 agtgataggc atccgtcagc caggacgccg gcagacgctc gaagtagcgg tagcccctcg
     3661 ccggccccag cttgcgcctc tgcgcctgca gagcctcccg cacggcgtgc gcgcgcccct
     3721 cgaaggcggc ggggtcaagc ccccagttct gcatgtcggc ggcgagggtt tcgttgatgg
     3781 cgaacggctc gtcgtaacgc agcgacggca gcgagccttt catcttcatc aggttgtggc
     3841 cggtctcgta gagctcgaag tcagtgttcc ggtagtcgtc gtcgtagtag tcgctgatct
     3901 gcgggtgggt ggcc
//
LOCUS       DBNHLM010000344         6276 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1129174, whole genome shotgun sequence.
ACCESSION   DBNHLM010000344 DBNHLM010000000
VERSION     DBNHLM010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6276)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1129174"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1692)
                     /gene="ligA"
                     /locus_tag="AC16QF_15830"
     CDS             complement(<1..1692)
                     /gene="ligA"
                     /locus_tag="AC16QF_15830"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR6450079.1"
                     /translation="MTITRAGAGSRISELRRLVRHHNHRYYVLDSPEIADAEYDALYD
                     ELVALEGDHPDLVTDDSPTQRVGGAPLEQFNSVTHEVAMLSLDKCTTHEELADWIERC
                     RGRLEEGEALDFVCEPKVDGVAVALTYENGRLVLGATRGDGQTGEDITANVRTVGAIP
                     LVMRDDAGHDFPPRIEVRGEIYMPVADFEAFNEQARQRDEKTLVNPRNGAAGSLRQLD
                     PKVSASRPLTMYCYSIGWVEGDWQPRTHMEALERLADWGFRVNPDARAVSGLAGCLRY
                     VEEMLERRDSLGYDIDGVVIKVNDLDQQRRLGAVTRKPRWSIAFKYPAEEATTTLIGV
                     EFQVGRTGAITPVARLAPVFVGGVTVSNATLHNMDEVRRLDIRVGDTVMVRRAGDVIP
                     QVVSVIDSRRPPGAEAVALPQGCPSCGSPITRPDDEAVARCSAGPEICPAQRKEGLRH
                     FASRLALDIEGLGDKLIEQLVDKDQVRIAADLFSLRKDELIRLERMGAKSAENLLAAI
                     ERSRETTLARFIYALGIREVGEATAQSLARHFGDLEPLRRAQVAELEQVPDVGPVV"
     gene            complement(1689..2642)
                     /gene="zipA"
                     /locus_tag="AC16QF_15835"
     CDS             complement(1689..2642)
                     /gene="zipA"
                     /locus_tag="AC16QF_15835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HIR6450080.1"
                     /translation="MDIKDFILIGGGLLIAGVIAHGFYIAWRGRRDTLRMDIAHDLIP
                     EAVDDLHRLRGELPNGGARVVSQASLGEQASLELDTVVVAEPLDAQPPEPAMLAEESA
                     VGESVKAREPTIGADDAPRTPAGEKDQKRPGAPRVADVTVAPEPIVAERPRRTRRPPA
                     RKAQETAPEPALEQPPVEELVVMNVLAPRGQPFAGDELFASLRGRGLKFGNMNIFHRL
                     DAGSKKVQFSVANIVEPGTFEMSDMETIRSPGLCFFLQLPGPENPLEAFETMLSVAHG
                     VAEELNGELKDEQRSFMTPQTEEHYRQRILEFSRRRMSKRA"
     gene            complement(2708..6208)
                     /gene="smc"
                     /locus_tag="AC16QF_15840"
     CDS             complement(2708..6208)
                     /gene="smc"
                     /locus_tag="AC16QF_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019363838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR6450081.1"
                     /translation="MRLSKIKLAGFKSFVDPTTITFPSSLVGVVGPNGCGKSNVIDAV
                     RWVMGESSASRLRGDSITDVIFNGSSARKPVGAASVELLFDNSDTTLEGQYAKYAEIG
                     IRREVSRDGISTYYLNGTRCRRKDITGVFLGTGLGPRSYSIIEQGMISQLVEAKPEDL
                     RVYLEEAAGISKYKERRRETENRVRHTRENLDRLNDIREELDRQLQHLKRQSRAAERY
                     RELKEEERKLTAELHTIRLVALEAVLDAHRERIQQLEVELSRRQAEQQAIDTQIEQGR
                     QAHADTSAAVNQVQGTFYQLGADIARIEEAIQFNQQRVKQLELDLETVTRRSEETGRQ
                     LAMDETQIRELREQLAELEPRRQEAERGDGLAAARLAELEQKHRAWQAEWDEFRNRAS
                     QNERDAEVQASRVEHLDQLLQRLRGRHDQLLDDAQEVPVLASDDVASLAEEIAGIESR
                     RSGLEADTDRTIKALGSIREDLLVRERMVEEARGELQSLRHDLASLQAVQRAALGRDE
                     RSAQDWVDARGLGSAERLGEALAVVPGWEHAVESVLGDDLQGIRVDDVSPFGVALDHL
                     PGAGVTLVEGSFHAESSGELPSLSSLIRSGSLKLDSLLYGIFAAESVEVAFAHRGGLR
                     AGQSIITRQGIWVGPDWVRILPVSEQDSGIIQRTQELETLGLRVEEAELGLAELQRQV
                     VEGRGQAEELEHDRAKLQAEVNDLNVRLGDMKAQHGVKRVQIEEAGARRERIRREREE
                     LDAQVSEETRKLLEAREALAIAERTREQQDVTREELQARGAASESELAAAREEARANR
                     ERYHALNAEWQSLESRMSASETARGRLVRQRRELDEQRDNLQAGIASSATPLPELRQD
                     LEARLNERLEVERQLGAARAELEQLGERIRGLQAERGEAEAAVETVRSKLETTRVERQ
                     GLAVQQSNLTDQIAATGLGADEVREGLAEDAEEARWVELLERTDRRIQRLGPINLAAI
                     EEFEAQSERKVYLDKQNEDLEQALETLLGAIRRIDRETRARFKETFETVNRHLGELFP
                     KVFGGGHAYLELTGEDLLDTGVSLMARPPGKRNSSVHLLSGGEKAMTAVALIFAIFHL
                     NPSPVCMLDEVDAPLDDVNVMRFAELIKDMSTDVQFVVITHNKLTMEMADHLMGVTMN
                     EPGVSRLVSVDVEEASAMAG"
BASE COUNT          992 a         2162 c         1953 g         1169 t
ORIGIN      
        1 gaccaccggg cccacgtccg gcacttgttc gagctcggct acctgcgccc gccgaagggg
       61 ttccagatcg ccgaaatgac gggccaggct ctgggcagtc gcttccccca cctcgcgaat
      121 gcccagagcg tagatgaaac gcgcaagcgt ggtctcgcgg ctgcgctcga tggcggccag
      181 caggttctcg gccgacttgg cgcccatgcg ctccagacgg atcagctcgt ccttgcgcag
      241 gctgaagaga tccgcagcga tcctcacctg atccttgtcc accagctgct cgatgagctt
      301 gtcgccgagg ccctcgatgt ccagtgccag acggctggca aagtggcgca ggccctcttt
      361 gcgctgggcg ggacagatct cggggccggc cgagcagcgc gcaactgcct cgtcgtccgg
      421 gcgcgtgatg ggactgccac acgacgggca cccctgaggc agcgccaccg cttcggcgcc
      481 gggcgggcgt ctggaatcga tgacgctgac gacctgggga atgacgtcgc ccgcccgcct
      541 caccatgacg gtgtcgccca cgcggatgtc caggcgccgg acctcgtcca tgttgtggag
      601 cgtcgcgttg ctcaccgtca cgccgcccac gaagacgggc gccagacgag ctaccggggt
      661 aatggctccg gtacgaccca cctgaaactc cacgccaatc agggtcgtgg tcgcttcttc
      721 cgccgggtac ttgaaggcga tggaccatcg cggcttgcgg gtgacggccc ccaggcgccg
      781 ctgctgatcg aggtcgttca ccttgatcac gacaccatcg atgtcgtagc ccagcgagtc
      841 gcggcgctcc agcatttcct ccacgtaccg caggcatccg gcgagcccgc tcaccgcacg
      901 ggcgtcggga ttgacacgaa aaccccagtc ggcgagacgc tccagggctt ccatgtgcgt
      961 ccggggctgc cagtctcctt ccacccagcc gatgctgtag cagtacatgg tcaacgggcg
     1021 gcttgcgctc accttcggat ccagctggcg aaggctgccc gcggctccgt tgcgcgggtt
     1081 gaccagcgtc ttctcgtcgc gctgacgcgc ttgctcgttg aacgcctcga aatcggcgac
     1141 gggcatgtag atctcgccgc gcacctcgat gcgcggcgga aagtcgtgcc ccgcatcgtc
     1201 ccgcattacc aggggaatcg cgccgacggt gcgaacgttg gcggtgatgt cctctccggt
     1261 ctgcccgtcc ccccgggtgg ctccgagaac cagccgaccg ttctcgtagg tcagggcgac
     1321 cgcaacgcca tccaccttgg gctcgcagac gaagtcgagc gcttctccct cttccagacg
     1381 tccgcgacaa cgctcgatcc agtccgcgag ctcctcgtgg gtcgtgcact tgtccaggga
     1441 gagcatggcc acctcgtggg tgaccgagtt gaactgctcg agcggcgccc ccccgacccg
     1501 ctgggtgggc gaatcgtcgg tcacgagatc gggatgatcg ccctccagag caacgagctc
     1561 gtcgtacaac gcgtcgtact ccgcgtcggc gatctctgga gaatcgagca cgtagtaacg
     1621 atggttatgg tggcgtacca gccgacggag ctcgctgatg cggctcccgg ctccggccct
     1681 ggtgatggtc atgcccgctt ggacatgcgc cgccgcgaga actccaggat ccgctggcgg
     1741 tagtgctcct cggtctgggg cgtcatgaag ctgcgctgct cgtctttcag ctcgccgttg
     1801 agctcttcgg cgacgccgtg ggcaacagac agcatggtct caaacgcctc gagcggattt
     1861 tcaggtcccg gcagctgcag aaagaagcag agtcccggcg agcggatggt ttccatgtcc
     1921 gacatctcga acgtccccgg ctcgacgatg ttcgccacgc tgaactgaac tttcttggag
     1981 cccgcatcca aacgatgaaa gatgttcatg ttgccgaact tcaggccgcg tcctcgcagc
     2041 gacgcgaaga gctcgtcgcc cgcaaacggc tgcccacggg gcgcgagcac gttcatcacc
     2101 acgagttctt ccaccggcgg ctgctcgagc gcaggctcag gagccgtttc ctgggccttg
     2161 cgggccggcg ggcggcgcgt gcgccgcggt ctttccgcga cgatgggctc cggggccacg
     2221 gtgacgtcgg ccacccgcgg ggcgcccggg cgtttctgat ctttttctcc ggcaggcgtc
     2281 cttggcgcgt cgtccgctcc gatggtgggc tctcgtgcct tcaccgactc cccgaccgcg
     2341 ctctcttcgg ccagcatcgc aggctcgggc ggctgcgcgt ccaagggctc agccaccacc
     2401 acggtgtcga gctcgaggct tgcctgctca ccgagagacg cctgcgacac cacgcgggcg
     2461 cccccgttgg gcagctcgcc gcgcaaccga tgaagatcgt cgacggcctc gggaatcagg
     2521 tcgtgagcaa tgtccatgcg cagggtatcg cgacgaccgc gccaggcaat gtagaacccg
     2581 tgggcaatca ccccggcgat tagcaggccg ccgccgatca gaatgaaatc tttgatgtcc
     2641 acaaccggct cctcggccgc ctgggcgacc tgacacgtcc agagggtgaa gcttccggcg
     2701 acatggctca gccagccatc gccgaggctt cttctacgtc taccgagacg agccgcgata
     2761 cgccgggctc gttcatggta acgcccatga gatggtcggc catctccatg gtgagcttgt
     2821 tgtgggtgat cacaacaaac tgcacgtcgg tagacatatc cttaatcagc tcggcaaaac
     2881 gcatgacgtt cacgtcgtcg agcggcgcgt ccacctcgtc gagcatgcag accgggctcg
     2941 ggttgaggtg gaagatggca aaaatcagag ccaccgccgt catcgccttc tccccgcccg
     3001 aaagcaggtg aacgctggaa ttgcgtttgc ccggcggcct cgccatgagc gaaaccccgg
     3061 tgtcgagcag atcctcccct gtcagctcca gataggcgtg tcctccgccg aatactttcg
     3121 ggaacagctc tcccagatgt ctgttcaccg tctcaaaggt ttccttgaag cgtgcgcgag
     3181 tctcgcgatc gatcctgcga attgcgccga gcagggtttc cagcgcctgc tccaggtcct
     3241 cgttctgttt gtcgaggtag accttccgct ccgactgcgc ctcgaactcc tcgatggcag
     3301 ccaggttgat ggggccaagg cgctggatgc gccgatcggt cctttcgagc agctcgaccc
     3361 agcgggcctc ttccgcatcc tcggccagcc cctcgcgcac ctcgtccgcg ccgaggccgg
     3421 tggcggcgat ctgatccgtg aggttggact gctgcacggc gagtccctgg cgctcgaccc
     3481 gcgtggtctc gagcttcgag cgaacggtct ccaccgctgc ttccgcttcg ccacgctctg
     3541 cctgaagccc gcgaatccgc tcgccaagct gttcgagctc ggcgcgcgcc gcgccgagct
     3601 gccgctcaac ttccaaccgc tcgttcagcc tggcctccag gtcctggcgc agctcgggca
     3661 gcggcgtggc gctggaggcg atgccggcct gaaggttgtc acgctgctcg tccagctcgc
     3721 ggcgctggcg aaccagccgg ccccgcgcgg tctcgctggc cgacatgcgg gactccaggc
     3781 tctgccactc ggcgttgaga gcgtggtacc gctcacgatt ggcccgggct tcttcccggg
     3841 ccgcggcaag ctccgactcc gacgcggcac cacgagcttg gagctcctca cgggtgacgt
     3901 cctgttgctc tctggtccgc tcggcgatgg ccagggcttc ccttgcctcc agaagcttgc
     3961 gcgtctcctc ggaaacctga gcgtcgagct cctcgcgctc gcgacggatc cgctcccgac
     4021 gggcgccggc ctcctcgatc tgcacccttt tcacgccgtg ctgggctttc atgtcgccga
     4081 gccgaacgtt caggtcgttg acctctgcct gcagcttcgc tcgatcgtgt tcgagctcct
     4141 cggcctgccc gcgcccttcc acgacctggc gttgcagctc ggcaaggccg agctcggctt
     4201 cctcgacccg cagcccgagg gtttccagct cctgagtccg ctggatgatg cccgaatcct
     4261 gctcgctgac cggcaggata cggacccagt cgggtccgac ccagatcccc tggcgggtaa
     4321 tgatgctctg gccggcccgc aacccgccgc ggtgcgcaaa agccacctcg acggactccg
     4381 ccgcgaaaat cccataaagc agggaatcga gcttgagact cccggatcta atgagggagg
     4441 aaaggctcgg cagctctccg ctgctctcgg cgtggaaact tccttccacc agggtcacgc
     4501 cggcgcccgg caggtgatcc agagcaacgc cgaacgggct cacatcgtca acgcgtatgc
     4561 cctgaagatc gtcgcccagc accgattcaa cggcatgttc ccatccgggc accaccgcca
     4621 gagcttcacc cagacgctcg gccgagccga ggcctcgggc gtcgacccaa tcctgcgcag
     4681 aacgctcgtc acggcccagg gcggcacgct gaacggcctg caggctggcg agatcgtgtc
     4741 tgagcgactg cagctcacca cgggcctcct cgaccatgcg ctcccgaacc agcagatctt
     4801 cccggatgga gccaagggcc ttgatggtgc gatcggtgtc ggcctccagg ccgctccgcc
     4861 gcgattcgat cccggctatc tcttcggcca gggacgccac gtcgtcgctg gcaagaacgg
     4921 gcacctcctg ggcatcgtca agcagctggt cgtggcggcc tcgaagacgc tgaaggagct
     4981 gatcgaggtg ctccacgcgg gaggcctgaa cctctgcgtc cctttcgttc tgagacgctc
     5041 gattcctgaa ctcgtcccat tcagcctgcc acgcccggtg cttctgctcg agctcggcca
     5101 ggcgggcggc ggccaggcca tccccgcgct cggcctcctg ccggcgcggc tccagctcgg
     5161 cgagctgctc ccgcagctcg cgaatctgcg tctcgtccat ggccagctga cggcccgttt
     5221 cctccgagcg ccgggtaacc gtctccaggt cgagctcgag ctgcttgacc cgctgctggt
     5281 tgaactgaat ggcctcctcg atgcgcgcga tgtccgcacc gagctggtaa aaggtcccct
     5341 gaacctggtt gacggcggcg ctggtatcgg cgtgagcctg gcgaccctgc tcgatctgcg
     5401 tgtcgatggc ctgctgctcc gcctgccggc ggctcagctc gacctcgagc tgctggatcc
     5461 gctcccgatg ggcgtcgagc acggcttcga gcgccaccag acggatggtg tgcagctcgg
     5521 cagtcagctt gcgctcttcc tctttgagct ctcgatatcg ctccgccgcg cgggattgac
     5581 gcttgagatg ctgaagttgc ctgtcgagtt cctcacggat gtcgttgagc cggtccaggt
     5641 tttcccgggt atggcgaacc cggttctcgg tctcccggcg acgctccttg tacttcgaga
     5701 tcccggctgc ttcctcaagg tagacccgca ggtcttcggg tttcgcctcg acgagctggc
     5761 tgatcatccc ctgctcgatg atggagtagc tgcgcggacc caggcctgtg ccgaggaaga
     5821 cccccgtaat gtccttgcgg cgacaccgcg tgccgttcag gtagtaagtc gagatcccgt
     5881 cccggctgac ctcgcgccgg ataccgatct ccgcgtactt ggcatactgg ccttccaggg
     5941 tcgtatcgga attgtcgaac agcagctcga ccgaggcggc gcccaccggt ttccgggcgc
     6001 tggagccgtt gaagatcacg tccgtgatgg agtcgccgcg gagcctgctt gccgagcttt
     6061 cgcccatcac ccagcggacg gcgtcgatta cgttggattt accgcagcca ttggggccca
     6121 ccacgccgac gaggctgctg ggaaaggtaa tagtggtggg gtctacaaat gatttgaagc
     6181 cggcaagctt gatcttgctt agtcgcatgc gatggattgt gcccaatacc agagagttcg
     6241 ccgtagttta aaggaatttc caaaataaaa cgattc
//
LOCUS       DBNHLM010000345        10719 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1249955, whole genome shotgun sequence.
ACCESSION   DBNHLM010000345 DBNHLM010000000
VERSION     DBNHLM010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10719
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1249955"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="AC16QF_15845"
     CDS             complement(<1..815)
                     /locus_tag="AC16QF_15845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR6450082.1"
                     /translation="MYTARFLKSISLGIGLCLLSACDQTSMSAQSDGSAAHTEEQYLK
                     MSNAALNAIPDVVFYKDVDGVYLGGNQAWLELIGKSDIAPEGMLGKTDFDLFPKEVAE
                     GFREMDRAMLSQGRTTRNDEWVTYPDGTRVLLETVKTPFVDENGELLGVLGISRDVTH
                     REAVDSAGVAVATEAWYEALNAVFEGDSQPMKEIWSHGEDVVYMGPAGDYLIGWDAVE
                     KAWDIQTAQNLGGRVLPTRVNMIVGKDMALINCVEIGENQVDGETKTVEIRSST"
     gene            complement(976..1272)
                     /locus_tag="AC16QF_15850"
     CDS             complement(976..1272)
                     /locus_tag="AC16QF_15850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR6450083.1"
                     /translation="MLEIELTAAGTVTVGGRLDALEAPKAQTFLDAVDGDCLLDLSNL
                     EYVSSAGLGVLLKTHKRLAGAGGGLRLVNVRSHIFDIFSYSGFDKLFEITRAAD"
     gene            complement(1276..1677)
                     /locus_tag="AC16QF_15855"
     CDS             complement(1276..1677)
                     /locus_tag="AC16QF_15855"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6450084.1"
                     /translation="MKRRFRRTREELASIVAMTETFFASERIDPSLRHVVDLVMEELF
                     VNTISYNTGTDEDVLIEMTATGDGVEVSLVDFDVERFDPTLAGQVDVDAPLEDREPGG
                     LGIFLVLKMVDAINYEYHDRQSRTTFRVSGN"
     gene            complement(1674..2963)
                     /locus_tag="AC16QF_15860"
     CDS             complement(1674..2963)
                     /locus_tag="AC16QF_15860"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR6450085.1"
                     /translation="MDQNEISRESLVSILEIMHRLASPDAMPELLREIVEVGKLAIVA
                     ETGVLWLAEHAGQQLVMVVPSHEPPSRIDVGVTWAGRCARDQAISNVHECRDDALFQE
                     HPLHLEGMETRSLLNVPILGRDRSLLGVMQWLGASPGQFDQHDEWVGPALAAQAAVAI
                     QHAYMTDELLANAILSREVAVAREIQRSTLPDVMPTCPGYDVHGYFQPAEATGGDLYD
                     LVMINDHLFMLLGDATGHGFGPALSATQLQAMLRVSFRHGATLDEAYMHVNNQLSEDL
                     PDDRFVTAFMGFLDPRTHSVSYHSGGQGPILHFSAADLSCRWFKPTSFPVGALDIHDE
                     PSAAQIELAPGDVLALISDGVYEYPNPADEIFGQQRVAELFALHQGAPVESLCRRLLQ
                     AVSEFGEGMPQADDITMVLVRRLPEQASAGDSEITQS"
     gene            3181..4950
                     /locus_tag="AC16QF_15865"
     CDS             3181..4950
                     /locus_tag="AC16QF_15865"
                     /inference="COORDINATES: protein motif:HMM:NF013056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HIR6450086.1"
                     /translation="MSGGNDSTDLIRYSADETPPHGLSAVLGAQIVVLILAAIAITPI
                     IVLKAAGAEDQGADWVVFAALLVSGIATMLQARPIGPFGAGYVLFMGTSGAFIAVSIT
                     AIQAGGLPLLGTLVATSALLQFLFALRLGWFRKIVNPTVGGTVITLIAVTVFPIGFGM
                     ISDVPRNFSGHELSPVWTAIGTLVGILVISLFGTGKLRLWGPMIGVVFGCAIAYPLDL
                     IDFSLVSQASWVGLPSSGWPGLDLSFGLAYWTLLPSFLIVTLVGALETFGDGIAIQQI
                     SHRERRPTDFKVVQGAVNADGMGNLLSGLMGTVPCTTYSTSISVVDLTGVGARRVGLY
                     GGGLLALLAFSPKLSALLQSIPGPVMGAFIVVLLVLLFAHGIRLILSSGLSFDNGIVF
                     GLAFWIGVGFQNQQIFSEFLPPVVLQILGNGMTAGGITAILLSWVVSLKSRRARRISV
                     PLAAESLADLHAFARENALKLGWRGDDLSRLELVVEEAFFYQLAQASEAAGHRLFTLL
                     MRPSGAELELEMISAPGDANMESLMASLSEAPEDPMDDIHLRILKHTVNSLSHQQFHN
                     AEYLLMHLGRSQSPDLAVAEDAH"
     gene            complement(5089..6003)
                     /locus_tag="AC16QF_15870"
     CDS             complement(5089..6003)
                     /locus_tag="AC16QF_15870"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HIR6450087.1"
                     /translation="MSQPSATPHLPSTRQVRYFAALAEHGHFGRAAASCHVSQSAFSV
                     AIKELETLLGVQLVDRTNRKVTVTAAGREVAVLARLCLQDLGALVEAARGASGPLEGP
                     LHLGVIPTIAPFLLPRALPAIRRAYPELKLFLYEELTATLYSDLMDGSIDVALLALPW
                     SLPGAETLELFRDPFRLAARKGTSLVDPERYRFNVLPAGSVLLLREGHCLREHAVEAC
                     RIRETEKMSRVSASSLLTLIEMVDADLGITFLPEMAEGSALLARTQVKTYPLKRDNYR
                     TIGLAWRKGTARAEEFAELGRLFSKHRP"
     gene            6108..6578
                     /locus_tag="AC16QF_15875"
     CDS             6108..6578
                     /locus_tag="AC16QF_15875"
                     /EC_number="1.16.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020302708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HIR6450088.1"
                     /translation="MSIDIGIPEKQRADIANGLSRLLADSYTLYLKTHNYHWNVTGPL
                     FNTLHLMFETQYTELSAAVDQIAERIRALGMPAPGSYSAFEELTSIEEERGQPSAEEM
                     IRQLVIGQETVARTAREIFPLADQANDEPTADLLTQRMQLHEKNAWMLRSMLER"
     gene            6608..7348
                     /locus_tag="AC16QF_15880"
     CDS             6608..7348
                     /locus_tag="AC16QF_15880"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIR6450089.1"
                     /translation="MKNLEGQKVPQVSFRTRKGSDWINRTTDEIFAGRTVVVFSLPGA
                     FTPTCSSSHVPRFNQLAGAFARHGVDEIVCISVNDTFVMNEWQESQKAWDVTFLPDGN
                     GEFTEKMGMLVRKDDLGFGARSWRYSMLVRDGVIEKMFIEPEIPGDPYEVSDADTMLD
                     YLAPNEAHPASVTLFTRKGCAHCVRAKAMLEDHGYEYEELVLNRDYTDRTLRAVAGAT
                     SLPQVFIDGRLVGGADDLEAVLAEDRAA"
     gene            complement(7459..8010)
                     /locus_tag="AC16QF_15885"
     CDS             complement(7459..8010)
                     /locus_tag="AC16QF_15885"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6450090.1"
                     /translation="MSNEEYSPKLSLAHATLSVTRFEEALDFYCDVLGFQITNSGEVP
                     GGGKMAFISQDPNNHHQIAMVEAAEPGSHAFVMADHLAFRTGSLQDLRILRERLTAAG
                     IETILPICHGNAWSLYFSDPEGNGLECFVDTPFHVAQPFAQGFDLDQPDEEIAAATKA
                     LIESEPEFQTMANWKAAFENRLG"
     gene            complement(8007..9545)
                     /locus_tag="AC16QF_15890"
     CDS             complement(8007..9545)
                     /locus_tag="AC16QF_15890"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004065 - arylsulfatase activity [Evidence
                     IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase B"
                     /protein_id="HIR6450091.1"
                     /translation="MRASSDRHGYTRRAAWFGCCMALSFASAALGSTQTGPNIVLILA
                     DDLGWNDVGYHGSEISTPNIDALAAQGVVLNQFHSQPTCTPTRAALMTGKSPQRMGIY
                     RQFAKISTEGLPVGEKTLADHLRAAGYQTWLTGKWHLGHSRAEFHPNARGFDHFYGHV
                     TGGIGYWDHVHGGGLDWQRNGRTLREEGYSTHLIAEEAVRLVRTRQPSRPFFLYASFN
                     APHLPNEAPEKAMQAYAGIENPQRRVHAAMVTELDAAVGEIVRTLEDEKLLDDTLIWF
                     MSDNGGLNRDAGLGGFLTLAEALDDWFEAPVPIRFLEFIRTNALDGGSDNGPLRFGKG
                     SVYEGGVRVPSFVTFPPALDPHRSDAMVTVQDVLPTLLAAAALEPADGLDGRNRWPEL
                     RQEPGESPPADYVTIGQNGAQAYYRWPWKLIATNGQHELYNLETDPLEEQNLADVETE
                     VVSTLALELSQLPRAESLHVPVYRVVWDPDFFGGEEDRQPWADIIDRAMHEQRAVKTG
                     GYLP"
     gene            complement(9529..10008)
                     /locus_tag="AC16QF_15895"
     CDS             complement(9529..10008)
                     /locus_tag="AC16QF_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6450092.1"
                     /translation="MDLGINGRHAIVCASSRGLGKACEAPDILVTNNAGPPPGAFADW
                     GPDEWQAALEANLLAPTFMIQGVIEGMKERRFGRIVNITSAMVKSPHAAMGLSTAARS
                     GLTFEDARQAIANTINAKRFGQPEELGDACAYLCSAQAGFICGQNLQLDGGSYAGLI"
     gene            complement(10010..>10719)
                     /locus_tag="AC16QF_15900"
     CDS             complement(10010..>10719)
                     /locus_tag="AC16QF_15900"
                     /inference="COORDINATES: protein motif:HMM:NF044751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6450093.1"
                     /translation="PPTGGLGLNTGIADAHNLAWKLAMVLHEQADPSLLDTYDGERRP
                     IAQINSHQSVTNSAKLLHLFGALYGPDPAATPEHYASACADPTAFPEISQNVEIQRPH
                     FDSFNLQLGYRYGATVDPDTMDISRYVPAFEVGDHLPHVRLQDGSWLVGKLPVNGFSL
                     ITGPKAQAWAKVACPVFSEDVDFKADQRAFFEQAGLHRDGALLLRPDGHICARWLKAP
                     EDPQAAVDEQVNEATGR"
BASE COUNT         1910 a         3268 c         3369 g         2172 t
ORIGIN      
        1 gtcgatgatc ggatctcgac cgtttttgtt tcaccatcta cctggttttc gccgatctcg
       61 acgcagttga tcaacgccat gtccttgccg acgatcatat tgacacgtgt gggtaaaaca
      121 cggccgccca gattctgcgc tgtctggatg tcccaggctt tctcaacggc atcccagccg
      181 atcagatagt cgccggccgg gcccatatag acgacgtcct ctccgtggga ccagatctct
      241 ttcatcggct gactgtcgcc ctcgaacacc gcattgagag cttcgtacca agcctctgtt
      301 gcgacggcga cgccggcgct gtcgactgcc tcccggtgcg tgacgtcacg gctgatgccg
      361 agaaccccaa gcagttcccc gttctcatcg acgaatgggg tcttgacggt ctcgagcagc
      421 acgcgggttc cgtcggggta ggtgacccac tcgtcattgc gtgtggtgcg tccttggctg
      481 agcatggcgc gatccatctc gcggaagccc tcggcaactt ccttcgggaa aagatcgaag
      541 tcggtcttgc ccagcatacc ctcgggggcg atatccgatt tcccgatcag ctcgagccat
      601 gcctggttgc cgccgagata aacgccgtcc acgtccttgt agaaaactac gtcaggaatg
      661 gcattcagcg cagcgttcga catcttcaaa tactgttctt cagtatgcgc tgccgaaccg
      721 tccgattgtg cggacatgct cgtctggtcg catgcgctga gcaggcagag gccgatgccc
      781 agagaaatcg acttgaggaa tcttgctgtg tacatgttgg ttctctaatc tgtgaattga
      841 agtttgccgt catcggttcg gcggatccat ttctggaagc cgccgcggaa atcagattgt
      901 ggattcgtca tcctctccgt tcgtgcgtag atcagtcctg tcccgttgat tgtgctgttt
      961 gaggaggctg cgtcatcaat cggctgccct ggtgatctcg aagagcttgt cgaagcctga
     1021 gtaactgaag atgtcgaaga tgtggcttct gacgttgacc agcctgagcc caccgccggc
     1081 cccggccaga cgtttgtgcg tcttgaggag cacgccgagt ccagcgcttg atacatactc
     1141 cagattggag agatccagca aacagtctcc gtccacggca tcaagaaagg tctgagcctt
     1201 tggcgcttcg agcgcgtcca gacggccgcc taccgtgacc gttcccgccg ccgtcagttc
     1261 gatttccagc atccgtcagt tcccacttac acgaaatgtc gttctactct ggcgatcatg
     1321 gtattcgtag ttgatggcgt cgaccatctt tagaaccagg aagataccga gaccgccggg
     1381 ttcccggtct tcaagcggcg cgtcgacgtc cacctgccct gcaagggtcg ggtcgaagcg
     1441 ctccacatcg aaatcgacga ggctcacctc tacaccgtcg ccggtggcgg tcatctcgat
     1501 cagcacgtcc tcgtcggttc ccgtgttgta cgagatcgta ttcacgaaca gctcctccat
     1561 caccagatcg accacgtggc gcagcgacgg gtcgatacgt tccgacgcga agaacgtctc
     1621 cgtcatggcc actatgctgg caagctcctc gcgtgttcgc cggaatctgc gcttcacgat
     1681 tgggtgattt ccgaatcgcc cgcggaagcc tgttccggga ggcggcgcac gagcaccatg
     1741 gtgatgtcat cggcctgggg cataccttcg ccgaactcgc taactgcctg cagcagccgg
     1801 cggcagagcg attccacggg ggcgccctga tgcagggcga aaagctcggc aacccgctgc
     1861 tgcccgaaga tctcgtcggc ggggttcggg tactcgtaca cgccgtcgga aatcagcgcg
     1921 agcacgtctc ccggagcgag ctcgatttgg gcagcgctcg gctcgtcgtg gatgtccagt
     1981 gcccccaccg ggaagctggt cggcttgaac cagcggcagc tcagatccgc cgcgctgaag
     2041 tgcagaatgg gtccctggcc gccggaatga tagctgaccg agtgcgtgcg agggtcgagg
     2101 aaacccatga atgccgtaac gaaccgatcg tcgggaagat cctcgctgag ctgattgttg
     2161 acgtgcatgt aggcctcatc cagggtcgcg ccgtgacgaa aggagacgcg cagcatggcc
     2221 tgcagctggg tggctgacag ggcgggtccg aacccatgtc cggtcgcgtc ccctagcagc
     2281 atgaagaggt ggtcgttgat catcacgagg tcgtagagat cgccgccggt tgcctccgcc
     2341 ggctgaaaat agccgtgcac gtcgtatccc ggacaggtag gcatgacgtc gggaagcgtg
     2401 ctgcgctgga tttcgcgggc caccgcaacc tctctgctga ggatcgcgtt cgcgagcagc
     2461 tcgtcggtca tgtaggcatg ctggatggcc acggccgcct gggcggcgag cgccgggcca
     2521 acccactcgt cgtgctggtc gaactgccct ggcgatgctc cgagccactg catgacgccg
     2581 agaagagaac ggtctcgacc gagtatcgga acgttgagaa gggatcgagt ctccatgccc
     2641 tcgagatgaa gcggatgctc ctggaacaat gcatcatccc ggcattcgtg cacgttgcta
     2701 atcgcctggt cccgggcaca acggcccgcc caggtgacgc ccacgtcgat ccgagatggg
     2761 ggctcatgtg agggcacgac catcactagc tgttgccctg cgtgttcggc cagccagagc
     2821 acccccgtct cggcaacgat cgccagcttt cccacctcca cgatctcgcg caggagttcg
     2881 ggcatggcat ctggcgacgc aagccgatgc atgatctcga gaatgctgac gagagactcc
     2941 ctcgagattt cgttctgatc catggcttct ggcataggca aggcctctgt ggcagctcgc
     3001 cagtatacgc cgatcgcgca gtaactagtt cttggcccag gcgaatattg ctggagcggc
     3061 atttggcgcg cgtatatgct gatcagttgt ggtcgcgggc ggcggcattg cccgacgacc
     3121 gccggtggtc gctaagataa gaacaacaag agaacaacaa gtcaaacgac gggggctcaa
     3181 atgagcgggg gcaacgactc gacggacttg attcgctact cggcggatga aactcctcca
     3241 catggcctct cggccgtgct tggcgcacag atcgttgtgc tcatcctcgc cgcgattgcg
     3301 atcacgccga tcatcgtgct caaggcggcc ggtgcggagg accagggcgc tgactgggtc
     3361 gtattcgcag cgctgctcgt cagtgggatc gccacgatgc tgcaggccag gccgattggt
     3421 cctttcggcg cggggtacgt gcttttcatg ggcacgtcgg gcgccttcat cgccgtctcc
     3481 atcactgcca ttcaggccgg cggcctgccg ctgctcggca cgctggtggc cacctcggca
     3541 ttgctgcagt tcctgttcgc gctacgcctc ggctggttcc gcaagatcgt caatcccacg
     3601 gtgggcggaa ccgtgattac gctgatcgcc gtgaccgtgt ttcccattgg ttttggaatg
     3661 atttcggatg tgccgaggaa cttcagcggc catgagctca gcccggtgtg gactgccata
     3721 ggcacgctgg tcggcatact cgtcatctcg ctcttcggta ccggcaagct caggctctgg
     3781 ggccccatga tcggtgtcgt tttcggatgc gccatcgcct acccgttgga tctcatcgac
     3841 ttttcgctcg tcagccaggc gagctgggtc ggtttgccct cctcgggatg gccgggtttg
     3901 gacctgagtt tcggcctggc ttactggacg ctgcttccga gctttctgat cgtgaccctg
     3961 gtcggagcgc tggagacctt cggcgatggc atcgccattc agcaaatttc gcatcgcgag
     4021 cgtcggccga ccgatttcaa ggtcgtgcag ggcgccgtca acgcggacgg catgggcaac
     4081 ctgctgtcgg gcctgatggg cacggtgcct tgcaccacct actcgacgag tatctccgtc
     4141 gtcgacctca ccggggttgg ggcgcgaaga gtcggcctct acggcggcgg tttgctggct
     4201 ctgctcgcct tctcgcccaa gctgtcggct ctgctgcagt cgatccccgg acccgtgatg
     4261 ggtgcgttca tcgtggtgct gctggtgctt ctctttgctc acgggatccg gctgatcctg
     4321 tcgagcggcc tgtctttcga caacggaatc gtcttcggcc tcgccttctg gataggggtg
     4381 ggctttcaga atcagcagat cttttcggag ttcctgccgc cggtcgtgct ccagattctt
     4441 ggcaacggaa tgacggccgg cggtatcacg gccatcctgc tttcgtgggt cgtttctctc
     4501 aaatccagac gcgcgcgacg catctcggta ccactcgccg ccgaatccct cgccgacttg
     4561 catgcctttg ccagagaaaa cgcgttgaag cttggctggc gcggagatga cctttcgcgc
     4621 ctcgagctgg tcgtggagga agccttcttt tatcagcttg ctcaggcgag cgaggccgca
     4681 ggtcacagat tatttaccct tctgatgcgt cccagcggcg ctgagctcga gctggagatg
     4741 atcagcgcgc ccggtgacgc caacatggaa tcgctgatgg cttcgctcag cgaggcgccc
     4801 gaagacccca tggacgacat ccacctgcgc attctgaagc acacggtcaa ctcgctcagt
     4861 caccagcagt ttcacaacgc cgagtatctt ctaatgcatc tgggacgaag ccagagtccg
     4921 gatctggcgg tggcggagga cgctcattga attcaccgta atgcgtttcc agcgcctgag
     4981 gacctgcctg ccttgcccgg cgcgttcgca ggggtcaggg cagtcccggt gcgctggtct
     5041 cggtcatgtc gaaccggata atgcagcctt tggggccatc gttggccgtt acggccggtg
     5101 tttcgaaaaa agccgtccga gctccgcgaa ctcctccgcc cgcgcggtgc ccttgcgcca
     5161 ggcgaggccg atcgtgcggt agttgtcgcg cttgagcggg tacgtcttca cctgtgttct
     5221 ggcgagcaaa gccgatccct ccgccatttc gggcagaaac gtgatgccga ggtcggcgtc
     5281 caccatttcg atgagcgtca gcaggctgga ggccgagacg cggctcatct tctcggtctc
     5341 ccggatgcgg cacgcctcca ccgcgtgctc gcgaaggcag tggccttccc taagcagcag
     5401 cacgctgccg gcaggcagca cgttgaagcg atagcgctcg ggatctacca gggacgtgcc
     5461 tttccgggcg gcaagcctga acggatcgcg gaacagctcg agcgtctcgg cccccggcag
     5521 ggaccaaggt agcgccagca gcgcgacgtc gatactgccg tccatcagat cgctgtagag
     5581 cgttgcggtg agttcctcgt aaaggaacag cttgagctcc ggataggcgc gccgtatcgc
     5641 gggcagcgca cgcggcaaca ggaagggcgc tatcgtcggt ataaccccga gatgcagggg
     5701 tccctccaga ggtcccgaag ctccccgagc cgcttctacc agcgcaccga ggtcctgcag
     5761 gcagagcctg gcaagcacgg ccacctcgcg gccggcggcg gtgacggtca ctttgcggtt
     5821 cgtccggtcg acgagctgca cgccgagcag cgtctcgagt tccttgatgg cgacgctgaa
     5881 agctgactgc gatacgtgac agctcgcggc agcgcggccg aaatgaccat gctcggcaag
     5941 cgccgcgaag tagcgaacct ggcgggtcga cggcagatga ggggttgcgg acggttggct
     6001 caatgatatg taaaacagat caatttgata taaatattct gatttacgga tcattttcag
     6061 tcaatagact tctggtatct gctcaactga aaaacaagga ggtcgtaatg agcatcgaca
     6121 tcggaatccc ggaaaagcag cgcgccgaca tcgcaaacgg gctgtcgcga ctgctcgcgg
     6181 acagctacac gctgtatctc aagacgcaca actaccactg gaacgtgacg ggacctctgt
     6241 tcaacaccct gcacctgatg ttcgagactc agtacaccga gctctccgca gccgttgacc
     6301 agatcgccga gcgtatacgt gcccttggta tgccggcgcc cggttcgtac agcgccttcg
     6361 aggagctcac gtccatcgag gaggaacggg gacagccctc ggccgaggag atgatcaggc
     6421 agctggtcat cggccaggaa acggtagcgc gtacggcccg ggagatcttt cccctggctg
     6481 accaggccaa cgacgaaccg acggccgacc tgctgaccca gcgcatgcag ctgcatgaga
     6541 agaatgcctg gatgctgcgc agcatgctgg aacggtaaat tcattgacaa cacgaggaac
     6601 tgcaatcatg aagaatctgg aaggtcaaaa agtcccccag gtgtcgttcc gcacccgcaa
     6661 gggatctgac tggatcaatc gcaccacaga cgagatcttt gccggccgga cggtggtcgt
     6721 cttttcgctg cccggcgcgt tcacgccgac ctgctcatcg tcccatgtgc cgcgtttcaa
     6781 ccagctcgcc ggggctttcg cacgccacgg cgtggacgag atcgtctgca tttcggtcaa
     6841 cgacacgttc gtcatgaacg aatggcagga gagccagaag gcctgggacg tgacgtttct
     6901 gcccgatggc aatggcgagt tcaccgaaaa gatgggaatg ctggtccgta aggacgatct
     6961 tggtttcggc gcgcgctcgt ggcgctactc gatgctcgtc cgcgacgggg tgatcgagaa
     7021 gatgttcatc gagcccgaaa tcccgggtga cccttatgaa gtgtccgacg ccgacacgat
     7081 gctcgactac cttgcgccga acgaggcgca tccggcaagc gtgacgctgt ttacacgcaa
     7141 gggctgcgct cactgcgtac gggcgaaggc aatgctcgag gatcacggtt acgagtacga
     7201 ggagctcgtt ctcaaccgtg attacacgga ccggacgctg cgtgccgtgg cgggcgccac
     7261 gtcgttaccg caggtcttca tcgacggccg cctggtcggg ggggccgatg atctggaggc
     7321 cgttctggcg gaggatcggg cggcctaggg accgggcctg gcagggcgct catgcagacg
     7381 tgcctcttcc gggcgcgtgg cctgatcgcc cagccatcgc caacccgcct cgttcgatca
     7441 ccagcccctc gcgatggttc aacccaggcg gttctcgaac gctgccttcc agttggccat
     7501 ggtctggaac tcgggttccg actcgatgag cgccttcgtc gcggcggcga tctcctcgtc
     7561 gggttgatcc agatcaaagc cctgcgcgaa aggttgggcg acatggaaag gcgtgtcgac
     7621 gaagcattcg agaccgttgc cctccggatc gctgaagtac aaagaccacg cgttcccgtg
     7681 acagatcggc agtatggtct cgataccggc agccgtgagc cgctcgcgca gaattcgcag
     7741 gtcctgcagg gagccggttc ggaaagcgag gtggtcggcc atgacgaagg catggctgcc
     7801 gggctcggcg gcctccacca tggcgatctg gtgatggttg ttgggatcct ggctgatgaa
     7861 agccatcttt cccccgcccg ggacctcacc gctattggtg atctgaaaac ccagcacgtc
     7921 gcaataaaag tcgagcgcct cttcgaatct ggtgacgctc aacgtggcat gggccagcga
     7981 gagtttcggt gagtattctt cgttgctcat ggcaggtagc ctcctgtttt tacagctctt
     8041 tgttcatgca tcgcgcggtc gatgatgtcc gcccagggtt gccgatcctc ctctccgccg
     8101 aagaagtcgg ggtcccagac cacccggtaa acggggacgt gcaggctctc ggctctcggc
     8161 agctggctga gctcgagggc gagggtgctc acgacctcgg tctcgacatc ggccaggttc
     8221 tgctcttcca gcggatccgt ttccaggttg tagagctcgt gctggccgtt cgtcgcgatc
     8281 agcttccacg gccaccggta gtaggcctgg gcaccgttct ggccgatggt gacgtagtcg
     8341 gcgggcggtg actcgccggg ttcttgccgc aactcgggcc agcggttcct gccgtccaga
     8401 ccgtctgcgg gctcgagggc tgcggctgcc agaagcgtcg gcagcacgtc ctgcaccgtc
     8461 accatggcgt cggaacggtg gggatcaagt gctggcggga aggtgacgaa ggagggcacg
     8521 cgcacgccac cttcgtagac ggaacccttg ccgaaacgca gcggcccgtt gtcggatcct
     8581 ccgtctaaag cattggtgcg tatgaactcg agaaaccgaa tgggcaccgg cgcttcgaac
     8641 cagtcatcca gggcttcggc cagggtgagg aagcccccca accccgcgtc gcgattcagg
     8701 ccgccgttgt cactcatgaa ccagatcagg gtgtcgtcga gcaacttctc gtcctcgagc
     8761 gtgcggacaa tctctccaac ggcggcatcc agctcggtga ccatggctgc gtgcacgcgt
     8821 cgttgcggat tttcaatgcc cgcgtaggcc tgcatggctt tctcgggggc ctcgttcggc
     8881 aggtgaggtg cgttgaagct cgcatataga aagaacggtc ggctcggctg cctcgtgcgc
     8941 accagacgta cggcctcctc ggcgatcagg tgcgtgctgt atccctcctc acgcaaggtt
     9001 ctgccgttgc gctgccaatc cagcccgccg ccgtgcacgt ggtcccagta gccgatgccg
     9061 cccgtgacgt ggccgtagaa gtggtcgaag ccgcgggcgt tcgggtgaaa ttccgcccgg
     9121 ctgtgcccca gatgccactt tccggtgagc caggtctggt agccggccgc tcggaggtga
     9181 tctgccagcg tcttctcgcc gaccgggaga ccttcggtgc tgatcttcgc gaactgccgg
     9241 tagatgccca tcctttgggg cgacttgccg gtcatgagcg cagctcgcgt gggcgtgcag
     9301 gtgggctggc tgtgaaactg attcagcacc acgccctgcg cggctagggc gtcgatgttg
     9361 ggtgtcgaaa tctcgctgcc gtggtagccg acatcgttcc accccaggtc gtcggccagg
     9421 atcaggacga tgtttggccc cgtttgggtc gagcccagag cagccgaggc gaacgacaaa
     9481 gccatgcagc agccgaacca cgctgcccgg cgcgtgtagc cgtgacggtc agatgaggcc
     9541 cgcataggaa ccgccgtcca gctgtagatt ctggccgcag atgaacccgg cctgtgcgct
     9601 gcacagatag gcgcaggcat ctccgagctc ttccggctga ccgaagcgtt tggcattgat
     9661 ggtgtttgcg atggcctgtc gcgcatcctc aaaggtcagg ccgctgcgcg ctgccgtgga
     9721 caaccccatg gctgcgtggg gggacttcac catcgcggag gtgatgttga cgatgcgccc
     9781 gaaacgacgc tctttcatgc cctcgatgac cccctggatc atgaaggtgg gtgctaggag
     9841 gttcgcctct agtgcagcct gccattcgtc ggggccccag tcggcaaacg cgccgggggg
     9901 cgggccagcg ttgttggtga ccaggatgtc gggtgcttcg caggccttgc ccaaaccgcg
     9961 cgatgacgcg cagacgatgg cgtgacgacc gttgattcca aggtccatgt cagcgtcccg
    10021 ttgcctcgtt cacttgctcg tcgaccgccg cttgcggatc ttcgggggct ttcagccacc
    10081 gcgcgcagat atggccatcc gggcgcagca ggagcgcccc gtcgcggtgg agccctgcct
    10141 gctcgaaaaa ggcgcgttga tcggctttga agtcgacgtc ttccgagaac accgggcaag
    10201 cgaccttcgc ccaggcctga gctttcggtc cggtgatcag ggaaaagccg tttaccggca
    10261 gctttccgac caaccaggat ccgtcctgaa gccttacgtg gggaagatgg tcgccaactt
    10321 cgaaggcggg tacgtagcgg ctgatatcca tggtatccgg gtcgacggtt gcgccgtatc
    10381 gatagccgag ctgcaggttg aagctgtcga agtgaggtcg ttggatctcg acgttctggc
    10441 tgatttccgg gaacgcggtg ggatcggcgc atgccgaagc atagtgctcg ggcgtcgccg
    10501 ctggatcggg cccgtacagc gcgccgaaca gatgcagcag ctttgcggag ttcgtcacgc
    10561 tctggtggct gttgatctgc gcgatgggtc ggcgttctcc atcgtatgtg tccaggaggc
    10621 tcggatcggc ctgctcgtgc aacaccatcg ccagcttcca ggccaggttg tgcgcatcgg
    10681 caatgccggt attcaggccc agaccgccgg tgggtggaa
//
LOCUS       DBNHLM010000346         6109 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1250363, whole genome shotgun sequence.
ACCESSION   DBNHLM010000346 DBNHLM010000000
VERSION     DBNHLM010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6109
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1250363"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..144)
                     /locus_tag="AC16QF_15905"
     CDS             complement(<1..144)
                     /locus_tag="AC16QF_15905"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6450094.1"
                     /translation="MNIPIDAIRHAESLGYESIWTAEAYGSDAVTPAAWILAQTQKIK
                     VGTA"
     gene            complement(288..545)
                     /locus_tag="AC16QF_15910"
     CDS             complement(288..545)
                     /locus_tag="AC16QF_15910"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR6450095.1"
                     /translation="MTESYQLFQYDQCPFCRRVRQFMDAAGIDIEIRDTLRDPEALRE
                     LIRGGGRSMVPCLKIQSEEGTRWLYESADIITYLRDRFAAG"
     gene            679..1374
                     /locus_tag="AC16QF_15915"
     CDS             679..1374
                     /locus_tag="AC16QF_15915"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HIR6450096.1"
                     /translation="MSSRKKSDKKVTRVRMLIVGFTILVGIVVLGYGTLYSTGLGEGE
                     FLAGEHYRVIENPPRRRAGAPIMVREYFSYGCIHCRNFEPLLDEWRATQPERTAFERS
                     PVAFSPAWQLLAQTYFTLKRLDVLEQNHMRLFRAIHDNARQFATPESMADFVDGNGVT
                     RAEFLRMFNSPEVRGDLREADAIQRNIVISSVPTLVVDDRYVVNMDLGRKRALDVVDY
                     LISQQLAETAAAP"
     gene            complement(1401..3497)
                     /locus_tag="AC16QF_15920"
     CDS             complement(1401..3497)
                     /locus_tag="AC16QF_15920"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6450097.1"
                     /translation="MNINTRGLLAGGLLLLAPVVSAQADEFVLIEEIVVTSEFRPTAL
                     EDTAASVSVISLADQKAGALNHLEEVVGWAPNVNLASGGGRARFVQIRGIGERGQFAE
                     PLNSSVGLLLDGVDMSGVGTVATLFDVEQVEILRGPQGTLYGANALAGLINVVSADPS
                     DELYTRLSLDAGNYGAFGVGGVISGPLGDNVGYRLAVQSYGDDGFMDNRFLDRDDTDD
                     HDELTIRGKLHWQVGPETDLRLTLGHVDVDNGYDAFSLDNDRNTLSDAPGQDRQQSTF
                     GSAQLNTALGEAVTLQATVGYADSDIDYGYDEDWTYAGFDPIGYTSEDRYRRDRQTLN
                     FDVRLLSGQAARMPGSVDWVLGVYGLHQEVDLERDYTFFFGEPFASRFEVDRLAVYGE
                     LGVQMSQRIRASLGLRFEHHESDYSDSEAVAADPKDDMVGGRLVLEADVADDAMVYFS
                     ASRGYKAGGFNTSGTLERELRDFDPEKLWNYEVGVKGTSADGRLQARVAAFLMKRDDV
                     QVSTFIAVSREDGSTEFIDLIDNAAEGTNAGLEAEVLWMPTEQLEVFASFGWLDTEFD
                     DFINAAGEDLDGEEQAHAPGYQFHVWGEYRFADGWFLRLAVEGRDAFYFTDGRRFAPN
                     AEDLKSDSYELVHASAGYEADRWRVSAWGRNLTDEDYAVRGFYFGNDPRDFYTARGFT
                     QLGEPRRYGITLQLEL"
     regulatory      complement(3549..3660)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3763..4476
                     /locus_tag="AC16QF_15925"
     CDS             3763..4476
                     /locus_tag="AC16QF_15925"
                     /inference="COORDINATES: protein motif:HMM:NF024937.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HIR6450098.1"
                     /translation="MEEYQAVVQTIALTMGVAWASGLNLYAALAVLGLTGSLGYVDLP
                     AQLEIVQDPLVILAAGFMYCVEFMADKIPGVDTGWDALHTLVRIPAGALLAAGALGDV
                     SPALAVTGGILGGGVAAATHATKAGSRALINTSPEPFSNWGASILEDLAVIGGLWAAL
                     THPMIFLAAFTIFMLLLCWLLPRIWRGVRRVFRTVGGWLGLVDASPANLQAELMKLGE
                     AGVLTAAEVHNARRRLHAA"
     gene            4509..>6109
                     /locus_tag="AC16QF_15930"
     CDS             4509..>6109
                     /locus_tag="AC16QF_15930"
                     /inference="COORDINATES: protein motif:HMM:NF013106.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR6450099.1"
                     /translation="MAERISRRRKALGLLGIAVVLALAALTAYLVYLDRTITTTFEGR
                     RWTEPAVIYARPLELHPGAALTTAEVIAELDRLGYALQPSPPKPGAYHRSGDELDVYL
                     RAFDFPNGSRDAQRIMLKFSAGSLARVQDALGRPVPLIRLDPVTIGSFFPSHGEDRLV
                     LPPERVPDLLRETLKVVEDRNFDRHAGFDLRAIARAFWVNVTAGEVRQGGSTLTQQLV
                     KSYFLDNRRTIGRKLRELAMAIILDARFGKEDLLNAYVNEIYLGQDGRRAVHGFGLGA
                     QFYFNRPLTELDIQQLALLIAVIRGPSYYDPFRHPERARARRDLVLDKMLQGGLVDED
                     QHARAVRSPLTTVAGTRRGGAYYPAFLDLVRANLTALDRDALTTRGLAVFTTLEPQTQ
                     DDLEQAVAQALGDLESSRGLEPGSLQAAAVMTATQTGEVLALSGARSAGSDGFNRALN
                     ARRPVGSLLKPVVYLAALEQGLHLASLVDDAPVVLPLAGGTTWQPENFDHVTHGEVPM
                     IRALAESLNLATVNLGLEVGVGTVAA"
BASE COUNT         1060 a         2044 c         1947 g         1058 t
ORIGIN      
        1 ggccgtgccc accttgatct tctgcgtctg cgccaggatc caggccgccg gcgtcacggc
       61 gtccgagccg taggcctcgg cggtccagat ggattcgtag cctagactct cggcgtgtct
      121 gatcgcgtcg atgggaatgt tcatcttgcg tccggactag ccggaaagga ttccgagttt
      181 catatgagga gcccccaggt gtgtagtttc tggcgggacc gcgcccgccg aaggcgcagc
      241 aagttaacag accgcccaac ccacggcaac gcgagccccg agaccgctca gccggctgcg
      301 aagcggtccc gcaggtaggt gatgatgtcc gcgctctcgt agagccagcg ggtgccttcc
      361 tcggattgaa tcttcaggca gggaaccatg gaccgcccgc cgccgcgaat cagctcgcgg
      421 agcgcttcgg gatcacgaag ggtgtcgcgt atctcgatgt cgatgccggc cgcatccatg
      481 aactgacgga cccgacggca gaacgggcac tgatcgtact ggaacagctg ataggattcg
      541 gtcaaaggat tccgctcgcc gaaaaaaagc ggcgcagtat gccaccgcca ccgaaagtca
      601 tgcaaagggc gcgctacccg agtttccaca ccagcgtcgc tgtgggtaaa ctccggcgca
      661 aacgacaggg gaggccccat gtccagtcgc aaaaagtcgg acaagaaagt gacccgggtg
      721 cgtatgctga tcgtcggctt caccatcctg gtcggcatcg tggtgctggg gtacggcacg
      781 ctctacagca ccggcctggg cgaaggcgag tttctggccg gcgagcatta ccgggtcatc
      841 gagaacccgc ccagacggcg ggcgggcgcg cccatcatgg tgcgcgagta tttctcctac
      901 ggctgcatcc actgcaggaa tttcgagccg ctgctcgacg agtggcgagc gacgcagccg
      961 gaacggaccg ccttcgagcg ctcaccagtg gctttctcgc cggcctggca gctgctggcg
     1021 cagacctatt tcacgttgaa gcgcctggac gtgctggagc agaaccacat gcgtctgttc
     1081 cgagccattc acgataacgc tcggcagttc gcaacgccgg aatccatggc cgatttcgta
     1141 gacggcaatg gcgttacccg agccgaattt ctccggatgt tcaactcacc ggaagtgcgc
     1201 ggcgacctgc gcgaagcaga cgccatccag cgcaacatcg tcatctccag cgtccccacc
     1261 ctggtcgtgg acgacaggta cgtggtgaac atggacctgg ggcgcaaaag agcgctcgac
     1321 gtggtcgact atctgatctc ccagcagctt gcagagactg ccgccgcgcc ctgacgcggc
     1381 ctgcacggga ttgacgacgg tcagagctcc agctggaggg tgattccgta acggcgaggc
     1441 tcgccgagct gggtgaagcc gcgcgccgtg tagaaatccc gcggatcgtt gccgaaatag
     1501 aagcctcgta cggcgtagtc ctcgtccgtc agattgcggc cccaggcgga cacccgccac
     1561 cggtctgctt cgtaacccgc agaggcatgc accagctcgt agctgtccga cttcagatct
     1621 tcggcgttgg gcgcgaaccg gcggccgtcg gtgaagtaaa aagcgtccct gccctccacc
     1681 gccaggcgca ggaaccagcc gtccgcgaat cgatactcgc cccagacgtg aaactggtag
     1741 ccgggcgcgt gagcctgctc ctcgccatcg agatcctctc ccgcggcgtt gatgaaatcg
     1801 tcgaactcgg tatcgagcca gccgaagctg gcaaacacct ctagctgctc ggtgggcatc
     1861 cagaggacct cggcttccag cccggcgttg gtgccttcgg ccgcgttgtc gatgaggtcg
     1921 atgaactcgg tgctgccgtc ttcgcgggac acggcgatga aagtggacac ctgaacgtcg
     1981 tcccttttca tcagaaacgc cgcgacgcgc gcttggagcc ggccgtcggc cgacgtcccc
     2041 ttcacgccca cctcgtagtt ccagagcttt tcggggtcga aatcccgcag ctcgcgctcc
     2101 agggtgccgc tggtattgaa gccgccggcc ttgtagccgc ggctggcgct gaaatagacc
     2161 atggcgtcgt ccgcgacgtc cgcttccagc accaaccgac cgccgaccat gtcgtccttc
     2221 ggatcggccg ccaccgcctc ggaatccgag tagtcggact cgtggtgctc gaaccgaagc
     2281 cccaaagagg cccggatgcg ctgcgacatc tgcacgccga gctcgccgta gacggccagc
     2341 cggtccacct cgaaacggct ggcgaacggc tcgccgaaga agaaggtgta gtcgcgctcg
     2401 aggtccactt cctgatgcag gccgtacacg cccagcaccc agtcgacgga cccgggcatt
     2461 cgggctgcct gcccggaaag cagccggaca tcgaagttga gcgtctggcg atcgcgccgg
     2521 tagcggtctt cggaggtgta gccgatgggg tcgaaacccg cgtaggtcca gtcctcgtcg
     2581 taaccgtagt cgatgtcgct gtccgcatag cccacggtag cctgtagggt gacggcctcg
     2641 cccagcgcgg tgttcagctg agcgctgccg aacgtgctct gctggcggtc ctggcccggg
     2701 gcatcggaaa gcgtgttgcg gtcgttgtcg agcgagaacg catcgtagcc gttgtccacg
     2761 tccacgtggc ccagcgtgag tcgcagatcg gtttccgggc caacctgcca atggagcttg
     2821 ccgcggatgg tcagctcgtc gtggtcatcc gtgtcgtcgc gatccagaaa gcggttgtcc
     2881 atgaagccat cgtcaccgta gctctgcacg gccaggcgat agccgacgtt gtcccccagg
     2941 ggaccggaga tcacgccacc caccccgaac gccccgtagt tgccggcatc cagggagagc
     3001 cgcgtgtaga gctcgtcgct ggggtcggcg ctgaccacgt tgatgaggcc agccagggca
     3061 ttcgccccgt agagcgtccc ctggggcccg cgcagaattt ccacctgttc cacatcgaag
     3121 agcgtggcga cggtgccgac cccgctcata tctacgccgt cgagcagaag ccctaccgac
     3181 gagttgagcg gctcggcgaa ctggcccctc tcaccgatgc cgcggatctg cacgaatcgg
     3241 gcccggccgc cgccgctggc cagattcacg ttgggcgccc accccacaac ctcctcgaga
     3301 tggttgagag cccctgcttt ctggtcggcc agggagatca ccgacacgct cgccgcggtg
     3361 tcctccagcg ccgtggggcg gaactcgctg gttacgacga tctcctcgat gagaacgaac
     3421 tcgtcggcct gtgccgaaac caccggtgcc agcaacagca agccgccagc cagcagtcct
     3481 cgggtattga tgttcatggt ccacccactc aggttgcctt gttgcgaccg cgaaggggtg
     3541 aaatgacgga gaagagaagt gcctattcct acgccggaat taaccgaatc aggttcaaag
     3601 ggtccaccga cgagccgcgc tcgaccggtg tctcaggcag taccgcccct taggtctgca
     3661 gagattctcg cccggggtcc ttgcgggcgc aagcgcgtgc ttcagttagg gttcagattc
     3721 gccacgtaga gtgacgctga gaaaccgcag atccgaagcg aaatggaaga atatcaagca
     3781 gttgtgcaga ccatcgccct gaccatgggc gtggcgtggg cgagcgggct gaacctctac
     3841 gcggccttgg ccgtgctcgg ccttaccgga tcactgggct acgtggacct tccggcgcag
     3901 ctcgaaatcg tccaggaccc cctggtgata ctggccgccg ggttcatgta ctgcgtggag
     3961 ttcatggccg acaagatccc cggcgtggac acggggtggg atgcgctgca caccctggtc
     4021 aggattcccg ccggcgccct cctggcagcc ggcgcgctcg gcgacgtgtc gcccgcactg
     4081 gccgtgaccg gaggcatcct cggtggcggg gtggccgcgg cgacccatgc caccaaggcg
     4141 gggtcgcggg cgctgatcaa cacctccccc gagccgttca gcaactgggg cgcttccatc
     4201 ctcgaggacc ttgccgtgat cggcggcctc tgggcggcgc tcacgcaccc catgatattc
     4261 ttggccgcct tcacgatttt catgttgttg ctatgctggt tgttacccag gatatggcgt
     4321 ggggtgcggc gcgtgttccg cacggtgggc ggctggctgg gcctggtcga cgcctcgccc
     4381 gccaacctgc aggcggagct gatgaagctg ggcgaggcgg gcgttctcac cgcggcggaa
     4441 gttcacaacg caaggcgcag gcttcacgct gcgtgacatc gggccgccgg tcgtgatccc
     4501 gcgcctcgat ggctgaacgg atttcccgtc gacgcaaagc cctgggtctg ctgggtatcg
     4561 ccgttgtgct ggcactggcg gcactcacag cctatctggt ctatctggac cggacgatca
     4621 ccaccacctt cgaaggtcga cgctggacgg agccggccgt catctacgcc cggcccctgg
     4681 agctccatcc cggcgctgcg ctcaccaccg ccgaggtgat cgccgaactc gaccggctgg
     4741 gctacgcgtt gcagccatcc ccgccgaaac ccggcgctta ccaccgaagc ggagacgagc
     4801 tggacgttta cctgcgcgcc ttcgactttc ccaacggatc ccgggacgcc cagcggataa
     4861 tgctcaaatt ctctgccggg tccctggcgc gggttcagga cgccctcggc cgtccggtgc
     4921 ccttgatccg gctggacccg gtcaccatcg gcagcttctt tccttcccac ggcgaggacc
     4981 gcctggtgct cccgcccgag cgggtacccg atctgctgcg tgaaaccctc aaggtcgtag
     5041 aggatcgcaa cttcgaccgc cacgccggtt tcgacctgcg tgccatcgcc cgagcgttct
     5101 gggtaaacgt caccgccggg gaggtcagac aggggggcag cacgctgacc cagcagctgg
     5161 tgaagagcta tttcctcgac aaccggcgca ccatcggccg caagctgcgt gagctcgcca
     5221 tggcgatcat cctggacgcc cgcttcggca aggaagatct cctcaacgcc tacgtcaacg
     5281 agatttacct gggtcaggac gggcgccgcg ccgttcacgg cttcgggctc ggcgcccagt
     5341 tctacttcaa ccgtcccctg acagagctgg acatccaaca gcttgcgctg ctcatcgcgg
     5401 tgatcagagg cccctcctac tacgaccctt ttcgacatcc cgagcgggcg cgggcgcgcc
     5461 gggatctggt cctcgacaag atgcttcagg gcggcctcgt cgacgaagac cagcacgcac
     5521 gggcagtgcg atcacccctg accacggtcg cgggcacccg ccgcggcggg gcctactatc
     5581 cggccttcct ggatctggtc cgcgccaacc tgaccgccct cgaccgcgat gcgttgacca
     5641 cccggggcct tgcggtcttc acgacgctcg agccccagac ccaggatgac ctggagcaag
     5701 cggtggctca ggctcttggg gatctggagt ccagccgggg gctggaaccc ggctccctgc
     5761 aggcggccgc cgtcatgacc gccacccaga ccggcgaggt gctggccttg agcggcgcgc
     5821 gcagcgcggg aagcgacggt ttcaaccggg cgctcaatgc caggcggccg gtcgggtccc
     5881 tgctcaagcc cgtggtctat ctggcggcgc tggagcaggg gcttcatctg gccagcctgg
     5941 tcgacgacgc gcccgtcgtg ctcccgcttg ccggcggcac cacctggcaa cccgaaaact
     6001 tcgatcacgt gacccacgga gaagtcccca tgatccgggc actggccgag tcgctgaatc
     6061 tcgccacggt caacctggga ctcgaggtgg gcgtggggac ggtggccgc
//
LOCUS       DBNHLM010000347        12942 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1130195, whole genome shotgun sequence.
ACCESSION   DBNHLM010000347 DBNHLM010000000
VERSION     DBNHLM010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12942)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12942)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12942
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1130195"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="AC16QF_15935"
     CDS             complement(<1..712)
                     /locus_tag="AC16QF_15935"
                     /inference="COORDINATES: protein motif:HMM:NF013954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HIR6450100.1"
                     /translation="MAGNINRFARAPCAALLLMAAACSEAPAPKPDRSYEFESHTDLE
                     ALFAELNYTPEAWADGNRDVPRLYLTSIPSRWRHRTADSLPVTRKKQLFFRLISPAAL
                     RANELVAADRARLLELAKGPGQLAPDDADWLANLAARYRLRDESAAGPEAIEELMLRV
                     DVLPPSLILAQAAEESGWGTSRFADVGNALFGQWAWDDGIIPLERREELGEYYVARFE
                     TPLDSIRAYMRNLNTHRAY"
     gene            complement(725..1279)
                     /locus_tag="AC16QF_15940"
     CDS             complement(725..1279)
                     /locus_tag="AC16QF_15940"
                     /inference="COORDINATES: protein motif:HMM:NF024978.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HIR6450101.1"
                     /translation="MRRCQWGWAVALVLTCAGCAAVAPIGADSDYDSSANFTSYRSFG
                     WISDKPLLLTDATLTSPQFQGRAMKAIRQALEARGYGFEADVENADFAVAFTLGTREQ
                     IRVNAYPDQVRETWTWGGPVPQAADVRNYTEGTLSIDLFDVRLRRPVWHGWATRTISY
                     ADRTNPSPVIAEVVEAILAQFPPQ"
     gene            1392..2291
                     /locus_tag="AC16QF_15945"
     CDS             1392..2291
                     /locus_tag="AC16QF_15945"
                     /inference="COORDINATES: protein motif:HMM:NF024946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HIR6450102.1"
                     /translation="MGERSIFSVCCLILLTTQAARANDVEPRLYSAVPTGANFLSVGY
                     VRSEGEVSLDSSIPVIDAKGEVDSLVLQYSRGLNVAGRSGLLSVVVPYANLALEGIYL
                     GQPASGEVRGFGDPVVRLAVNFTGAPAMTGDEFRGYQQKTIVGASVAVSVPVGRYETE
                     RVLNTSSNRWNVVGQVGFSHRVRRWTLEGAVGLSAFSDNDELKGDNELSQESITLFRG
                     TALYHFTPRVWMSLGVVHSRGGATSLNGVKRRDHQNNWRSGLAFSFPVGRRHRMQLVA
                     TEGVSARIGADFTTFIANYTLSF"
     gene            complement(2346..4562)
                     /locus_tag="AC16QF_15950"
     CDS             complement(2346..4562)
                     /locus_tag="AC16QF_15950"
                     /inference="COORDINATES: protein motif:HMM:NF025875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6450103.1"
                     /translation="MVPIRAALLPLIVSFLSGCDARPPVATDTPDHELEPSTAALTYV
                     GSQQCAGCHAGQHDAWQRSHHRKAMEKPGSHSIVGNFDGASFVHDGTTTEFRAESGSY
                     VVYTDGENGEIARFPVAYTFGVEPLQQYLLPLPRGRLQALSVAWDSRPREAGGQRWFH
                     LYPEDRVDHTDVLHWTQGSQNWNYMCADCHSTNVRKGYDETLDEYDTSFSEVTVGCEA
                     CHGPASAHVDWALTGDGQAGLPSLGGQTAEINACAPCHSRRSQIAEGFRPDLAYLDHY
                     VPALLDEGLYHSDGQILDEVYVYGSFLQSRMHAQGVSCSNCHDPHSAELRATGNDVCT
                     GCHSEAGRADFPTLTKARYDASAHHFHSDGGPGSQCVDCHMPARTYMQVDDRRDHSFR
                     VPRPDLTVELGVPNACTGCHEDRPASWARDVLAERFGKAEREHYGAVFDAARRADPSA
                     ERALAALAEDEARPAIVRATALSLMTAYDRGASSFALSAGLRDESPLVRLGALKGAER
                     WPPDRRWRSAAHLLEDDYLAVRLEAVRVLAPAAGSLPAASRERLNRGVSEYASAQALH
                     GDRAEAHTNMAGVYLNLGEPSLAERALSRAIQINPRWVPALINLADLYRATGRDAAGG
                     GFLDRALSAAPDSGDVLLAKALWLVRQRSQDEALTLLARAAAVEPTSTRIAYVYAAGL
                     HSNDRTQEALEVLDTILATRPGNRQLLEAAAAMARDNGQDGRALGYLEQLKAADGL"
     gene            complement(4660..5373)
                     /locus_tag="AC16QF_15955"
     CDS             complement(4660..5373)
                     /locus_tag="AC16QF_15955"
                     /inference="COORDINATES: protein motif:HMM:NF023197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HIR6450104.1"
                     /translation="MKKNSRIARFALPLAVLLLGACASTTPTINTEAAPDEMSYDGLY
                     PVKGTRVTQAWARSDLDLRSYNKILLQGAGIQYRPVKSTGYRGTAGRSGKNEFPLDEQ
                     QKERVAAAASEAFRSELAKIKSLEIVSEPGPDVLIVRGALLDVVSRVPPEPIGRGNTY
                     LDSVGEATLVIELADSQSGSVLVRAVDRRAAQQTGWMTESTSVTNAAEVRRLFSGWAR
                     LVRTALEELTDDLDIGEAQ"
     gene            5843..8245
                     /locus_tag="AC16QF_15960"
     CDS             5843..8245
                     /locus_tag="AC16QF_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450105.1"
                     /translation="MVIHAPQIRAWDDFEDIEGLAALELRTDDGAPGRFGTIAFRARA
                     VPDLEARVVHFDDIEVTSVKEQGEEISEEARALFVAAMDLGARDVPLDLVLSHLADGV
                     IPESTTGIRFEPPEILVSTAPAVIVLLHGEPALAPVGDRGALFAANTNWPLFRNGAEG
                     AWYLRDNDNWLTAADLDGPWAWAGELPASLVQLPETDDWRSTRAAAKAWSRPPESEPP
                     SVFVSTEPAELILIDGEPELVEIGTLGLSFVANTESPLFGYSERWYYLVSGRWFTSRA
                     LDGRWSAVLELPKAFAQIPEDHEKADVLAAVPDTPAARMAALDAQIPEKTEVPRDLSM
                     PETVTYVGEPVFEPIPGTNLKRATNTAYSVIQAGADYYLCYSGMWYAASAPEGPWRVT
                     SHVPSQVYQIPPSSPVYNTTYVKVQEESPVSVTFGVTQGYSSVYVSNGVPVYGTGWYY
                     PPYYYYYGGYPIYFAYPYTYGSASFYNPATGTYGSVSRAYGPYGGWGYASAYNPTTGT
                     YGRAEAVWDHDEWYAVGEAYNPHTGRYYTTERYYDADDERWEIDSRFSGERGYVDVSR
                     EFDEDSGRATIETSRGGEGTFSREKSDGGWNTSGEFETAGGRTVTGSGRYEDGEGSST
                     LRGSEGGTGTIERSVDDGVVTRDGTFSKDGKTIETETTRDGTGRRTSFETSEGTSGAV
                     GGRGQQRTALAQTESGDLYAARDGNVYRKSDDGWSRHSDGSWETIPMPSRDADAGARS
                     YGGGDYRQSASSAYRGGADLDADRYRGLDRDYSARQRGMTRAGSYGGQRGSFGRLQGR
                     RR"
     gene            complement(8316..8699)
                     /locus_tag="AC16QF_15965"
     CDS             complement(8316..8699)
                     /locus_tag="AC16QF_15965"
                     /inference="COORDINATES: protein motif:HMM:NF028308.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HIR6450106.1"
                     /translation="MAQRHSRILTLLLLVVVAAPATAFWGWMRGSAANEFKESDWDLF
                     KAEMRQALEDGADGERFDWSNPKTGNSGSIRILETAEVEGETCRKAAFRNVTAKGTQG
                     QSVHYLCRQENGAWQFAAASAFESG"
     gene            8988..10526
                     /locus_tag="AC16QF_15970"
     CDS             8988..10526
                     /locus_tag="AC16QF_15970"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008031994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004065 - arylsulfatase activity [Evidence
                     IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HIR6450107.1"
                     /translation="MRRILLAMGASLLLPAMGLAADKPNILVIWGDDIGQSNISAYTR
                     GMMGYPTPNIDRIAAEGILFTDYYGEQSCTAGRSSFIMGQSVFRTGLSKVGLPGAEEG
                     MQVEDPSIAGLLKDQGYATGQFGKNHLGDRDEHLPTNHGFDEFFGNLYHLNAEEEPEN
                     EDYPKNPEFRERFGPRGVIRSSADGQIEDTGPLTKKRMETVDDETVAAAMDFMERQHA
                     AGKPFFVWWSGTRMHFRTHVKDEMRGISGQDEYSDGMVEHDMHVGMFLDKLDALGIAD
                     NTIIHYSTDNGPHYNTWPDAASTPFRGEKNTNWEGGWRVPAAVRWPGVVKPGTVSNGI
                     VHHMDWLPTFLAAAGKTDIKEDLLDGYRSPAMKRRYRVHLDGYDLTDHLKDPQGVESP
                     RKEIFYFSDDGDLTALRYQDWKLIFMEQRVEGTLQAWAEPFVPLRVPLLFNLRRDPYE
                     RSQKTSNTYYDWLIDRVFLMVPAQVYVGQFLATFREYPPRQKAASFSLERVLEQLQEG
                     GDSR"
     gene            complement(10632..11969)
                     /locus_tag="AC16QF_15975"
     CDS             complement(10632..11969)
                     /locus_tag="AC16QF_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450108.1"
                     /translation="MNIRNVSLALILAAVCVPSQATDTEEQQEFDSPLILKASEVLSA
                     ETRNGEHHEVREEIVNDGFMNTYVIDSPFGTFEAYGSSMLRTRINEIGALAELDGLSK
                     TKVFMEAAAQSAVRAVTAPVSAAATIVTNPIGTVTGLPGGLARMFNRQKRKVEGAVAT
                     TASAAKSATGADDSGEEASIAEQAGEGAVDLGKSLMGTTRAERRWAKKLGTDPYTSNE
                     TLRKGIADVAVVDSSGYFLAELAVPIGGIVDLVVDVNEAIWERDAYELRQMNLVKLQD
                     MGVAEEEATAFLDNVAISPTIQTLIINGLGLLETTAGRDAFLRQATLAEDEAESRFYL
                     QSLAMIAWLNQEGENVAAITVHGPVSIAVDTGGNRVAMLPVDHLTWNPTTVEAVGLWP
                     AQDPDVPSDGRVYISGDISALARSHLEQAGWSARSKIARDFFTYLSSQESDEP"
     gene            12201..>12942
                     /locus_tag="AC16QF_15980"
     CDS             12201..>12942
                     /locus_tag="AC16QF_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450109.1"
                     /translation="MTGLFLRALLAWLAVCPALVEGAEAVSPDTATAGEPGRPLPMES
                     DGKRNPLTPDVRDDDTELTMQRGDFVIAPIPISDPVLGEGLVLAAAYFYPQTEEQAAS
                     QPASVTAVAGMYTSSESYAYGIGQQNYWGEDRWRFSGALAHADLQLKLRPGAENGDGG
                     RTDWLIDGNVLQAELSRMIAGDWYLGLISRFVDINQSFETDRQTGNFDLGSSIRTVGM
                     GLSVEHDSRDLPLNSFSGRLFELRGLFND"
BASE COUNT         2175 a         4145 c         4294 g         2328 t
ORIGIN      
        1 ggtaggcccg gtgcgtgttc agattccgca tgtacgcgcg tatggaatcc agcggtgtct
       61 cgaagcgagc gacgtagtac tcgcccaact cctcacggcg ttccagagga atgatgccgt
      121 cgtcccatgc ccattggccg aacagggcgt tgcccacgtc ggcgaagcgc gacgtgcccc
      181 agccgctctc ctctgcggcc tgagccagga tcagagatgg gggcagaacg tccacgcgca
      241 gcatcagctc ctcgatggcc tccggcccgg cggcactttc gtccctcagc cggtaacggg
      301 cggccagatt cgccagccag tcggcatcat ccggtgccag ctgcccgggg cccttggcca
      361 actcgagcag ccgagctcga tcagcggcga ccagctcgtt tgcccgcagc gcggcaggac
      421 tgatcaggcg gaagaaaagc tgtttcttcc gggtgacggg gaggctgtcg gcggttcgat
      481 gccgccatcg gctcgggatg ctggtgaggt acagtcgggg cacatcccga ttgccgtccg
      541 cccaggcttc cggcgtgtag ttcagctctg cgaacagcgc ctcgaggtcc gtgtgggatt
      601 cgaactcata gcttctgtcc ggttttggag ccggcgcctc ggagcaggcg gcggccatca
      661 acagcagcgc tgcgcaaggt gcccgggcga aacgattgat gttgcctgcc attgccggtt
      721 ccggtcattg cggcggaaac tgcgccagga tcgcctcgac gacctcggcg atcaccggcg
      781 acgggttcgt ccgatcggcg taggagatcg tcctggtcgc ccacccgtgc catacggggc
      841 ggcgcaaacg cacgtcgaac aggtcgatgg acagggtgcc ctcggtatag ttccgcacgt
      901 ccgcggcttg aggcacgggc ccgccccagg tccaggtttc ccgaacctgg tccgggtagg
      961 cgttcacacg aatctgctcg cgcgttccca acgtaaaagc gaccgcgaaa tcggcgttct
     1021 cgacatcggc ctcgaagcca tagcccctgg cttccagagc ctgcctgatg gccttcattg
     1081 cccgtccctg aaactgcggg ctcgtcaagg tcgcgtcggt cagcagcagc ggcttgtcgg
     1141 atatccatcc gaagctccgg taggaggtga aattcgcgct gctgtcgtag tcgctgtcgg
     1201 cgccgatggg cgcgacggct gcgcacccgg cgcacgtcag gaccaaggcg acggcccagc
     1261 cccattggca tcgtctcatt tcggtgtcct cccgtggatc ccgtcgcggc gtccgcgcgc
     1321 cgtttccaga acgccgatgg tagcagtttt cgctatgatc acgggcctgc accgttaccg
     1381 gcgaggctgg cttgggagag cgctccatct tcagcgtttg ctgcctgatc ctgctgacga
     1441 cccaggcggc ccgcgcaaac gacgtcgagc cgcgcctgta ctcggcagta cccacggggg
     1501 ccaatttcct ctcggtgggc tacgtgcgat ccgagggtga agtgtctctg gacagcagca
     1561 ttcccgtgat cgatgcgaaa ggagaggtgg actccctggt cctccagtac tcgcgagggt
     1621 tgaacgtcgc cggcagatcg gggctgctca gcgtggtcgt gccatatgcc aacctggcgc
     1681 tcgaggggat ataccttggg cagccggcgt ccggcgaggt acgcggtttt ggagaccccg
     1741 tcgtgcgtct ggcggtgaac ttcaccggcg cgccggccat gaccggggac gagttccgcg
     1801 gctaccagca gaagaccatc gtcggtgcca gcgttgccgt ctcggtgccg gtggggcgct
     1861 acgaaaccga gcgtgtcctc aacacgagca gcaatcgctg gaacgtcgtc ggccaggtgg
     1921 gcttctcaca tcgggttcgt cggtggaccc tggaaggcgc cgtcggccta tcggcgttct
     1981 ccgacaacga cgagctcaag ggcgacaacg agctgtctca ggagtccata acgctgttcc
     2041 gcggcaccgc gctgtatcac ttcaccccgc gggtctggat gagccttggc gtggtacaca
     2101 gccgcggagg tgccaccagc ctcaacggtg taaagcgtcg cgatcatcag aacaactggc
     2161 gctcgggcct ggctttctcg ttccccgttg gccgcagaca caggatgcag ctggtggcca
     2221 cggaaggggt cagcgcgcga atcggcgccg acttcaccac tttcatcgcg aactacaccc
     2281 tctctttctg agtcctctgg ggagccgttg gcgcgcgtaa gatccgccag gcgggcaggg
     2341 ttcgctcaga gaccgtcggc tgcttttagc tgctccaggt atccgagcgc tcggccatcc
     2401 tggccgttat cccgggccat ggccgctgct gcttcgagca gctgtcggtt gccagggcgg
     2461 gtggcgagga tggtgtcgag cacctccagg gcctcctgcg ttcggtcgtt cgagtgaagc
     2521 ccggcggcgt acacgtaggc gattcgggtg ctggtcggct cgacggctgc tgcgcgggca
     2581 agcagggtga gggcctcgtc ctgagaccgt tgccgcacca gccacagcgc cttggcgagc
     2641 agcacgtccc cagagtcggg tgctgcgctg agcgcacggt ccagaaaccc gccccccgcg
     2701 gcatcacgcc ccgtcgctct gtacaggtcg gccaggttga tcagagccgg cacccaccgg
     2761 ggattgatct ggatggcccg ggatagcgcc cgttcggcca ggctcggttc gcccaggttc
     2821 agatagacgc cagccatgtt cgtgtgcgcc tccgcccgat ccccatgcag cgcctgagcg
     2881 gaagcgtatt ccgacacacc gcgattcagg cgttctcgtg atgccgccgg gagcgagcct
     2941 gctgccgggg ccagcacgcg gacggcctcc agccgcacgg cgaggtagtc gtcctccagc
     3001 aggtgcgccg ccgaacgcca gcggcgatcg ggcggccagc gctcggcccc tttcagggca
     3061 ccgaggcgca ccagggggct ttcgtccctc aagccggcgc tcagcgcaaa cgagctggcg
     3121 ccacggtcgt aagcggtcat cagggaaagc gcggtggcgc gaacgatggc cggccgggcc
     3181 tcatcctcgg ccagcgcggc gagcgctcgc tctgcgctcg gatccgcgcg acgggccgcg
     3241 tcgaaaacgg cgccgtagtg ctcgcgttca gccttgccaa agcgctctgc caacacgtcc
     3301 ctcgcccaac tggccgggcg atcctcgtgg cagccggtgc aggcgttcgg cacacccagc
     3361 tcgacggtca gatcaggccg gggaacccgg aagctgtgat ccctccgatc gtcgacctgc
     3421 atgtaggtgc gggcaggcat gtggcagtcc acgcattgcg agccagggcc tccatccgaa
     3481 tgaaaatggt gggcggatgc atcgtagcga gcctttgtga gcgtggggaa gtcggcccgg
     3541 cccgcttcgg agtggcaccc agtgcacacg tcgttgccag tggcgcgcag ctcggccgag
     3601 tgcgggtcgt ggcagttgct gcagctaacc ccttgtgcat gcatcctgct ctgcaggaac
     3661 gatccgtaga cgtacacctc gtcgagaatc tgcccgtcgc tgtggtagag cccctcgtcc
     3721 agcagggcgg gaacgtagtg atcgaggtag gcgagatccg gccggaaccc ctcggcgatc
     3781 tggctgcgtc ggctgtgaca gggcgcgcag gcgttgatct cggcggtctg acccccaagg
     3841 ctgggaaggc cggcttggcc gtcaccggtt agcgcccagt cgacgtgcgc ggacgccggg
     3901 ccatggcagg cctcgcagcc tacggttacc tcgctgaagc tcgtgtcgta ctcatccagc
     3961 gtctcgtcgt aacccttgcg tacgttggtg gagtgacagt cggcgcacat gtagttccag
     4021 ttctggctcc cctgggtcca gtgcagaacg tccgtgtgat ccacccgatc ctcgggatag
     4081 aggtggaacc agcgttgccc gccggcttcc cggggccgcg agtcccaggc cacgctcaac
     4141 gcctgcaggc gaccgcgcgg gagaggcagc agatactgtt gcagcggctc gacgccgaac
     4201 gtgtaggcga cggggaagcg ggcgatctcg ccgttctcgc catcggtgta caccacgtag
     4261 gaacccgatt ccgcgcgaaa ctcggtggtg gtcccgtcat gaacgaacga ggcgccgtcg
     4321 aaattgccga cgatgctgtg ggagcctggc ttttccatgg ctttgcgatg gtgcgagcgc
     4381 tgccaggcgt catgctggcc ggcgtgacag cccgcgcatt gctgggagcc aacgtatgtg
     4441 agcgccgccg tcgagggctc gagttcgtgg tccggggtgt cagtggcgac cggtgggcgg
     4501 gcatcgcagc ccgacaggaa gctcacaatc aggggcagga gagcggctcg gatcgggacc
     4561 atggcaggcg acggggtcag cgagcgttaa ccgtcaccca tcgccccggg aggacgcgat
     4621 gcgcctgaac ccggggcgag agggcggaag atggctcagc tattgggctt cgccgatgtc
     4681 cagatcgtcg gtcagctcct ccagggccgt gcgcaccagg cgagcccaac cgctgaacag
     4741 acgccgcact tccgcggcgt tggttaccga ggtggactcc gtcatccagc cggtctgctg
     4801 ggcggcgcgc cgatccacgg cgcgcaccag cacgctgccg ctttgggaat ccgccagctc
     4861 tatgaccagc gtcgcctcgc cgacagaatc caggtaggtg ttgcctcgac caatgggttc
     4921 cggcggtacc cgggacacca cgtcgagcag cgcgccgcgc acgatgagca cgtcgggccc
     4981 gggctccgaa acgatctcca ggcttttgat cttcgcaagc tcgctgcgga aagcttcgct
     5041 tgcggccgcg gcgacgcgct ctttctgctg ctcgtccagg ggaaactcgt tcttgccgct
     5101 gcgaccggcg gtgccccggt atccggtcga cttgaccggt cgatactgaa tgcccgctcc
     5161 ctgcagaaga atcttgttgt agcttctcag gtcgaggtcc gagcgggccc aggcctgggt
     5221 aacccgggtt cccttcacgg gatacaggcc gtcgtaggac atctcgtccg gggccgcctc
     5281 ggtgttgatg gttggtgtcg tgctcgcgca ggcccccagc agcaggacgg ccagcggcag
     5341 cgcgaacctg gcgatgcggc tgttcttttt catcgcggat tccccctttt ttgtcattgg
     5401 tggacggttg ctttccatgt cgtgaggggc cgatgttagc gtcaagctaa ccgaaagcaa
     5461 ggcggcctac tggcaggtgg cctcgcagcc cgcagcctgg gcgtcccctg ggcagtcacc
     5521 gcgttcgggt tgtcgtcgtg ggcacgatca cggctttcga ctacactgcc cggtctcctg
     5581 cgcagacccc ccgaggaacg ttatggaccg ctgggcacca acaggccttg cgttgatcag
     5641 ttcgctgttg cttgtgagcg cgtcgcccgt cccgtcgtgg ctgatgctga gttcggctca
     5701 ggcgtcagag ccggcggctg gcccgtctgc caccgcgtct gccaccgcgt ctgccaccgc
     5761 gtctgccacc gcgtctgccg gcaagactga agcgccgacg ccagggcagt ttccgaggtc
     5821 cgtggatgtc gagggcgggt tggtggtgat tcacgctccg cagattcgcg cgtgggatga
     5881 tttcgaggac atcgagggac tcgcggcgtt ggagctgcgc acggacgacg gcgcccccgg
     5941 ccggtttggc accatcgcgt tcagggcaag ggccgtcccg gacctggaag cccgcgtggt
     6001 ccactttgac gacatcgagg tcacctcggt gaaggagcag ggcgaagaaa tctccgagga
     6061 ggcacgggcg ctgttcgtgg cggccatgga tttgggggcc cgggacgttc cgctcgacct
     6121 ggtcctgagc cacctggctg atggggtgat cccggagtcc accacgggca tccgcttcga
     6181 gcctccagag atactcgttt ccaccgcccc agccgtgatc gtccttttgc atggcgagcc
     6241 ggcgctcgcg cctgtggggg atcggggagc gctgtttgct gccaatacca actggcccct
     6301 gttcagaaac ggcgccgagg gagcctggta cctgcgggac aacgacaact ggctcaccgc
     6361 cgccgatctc gacggtccgt gggcttgggc gggtgagctt ccggcctctc tggttcagct
     6421 gccggaaacg gacgactggc gctccacccg cgcagcggct aaggcatggt cgcggccgcc
     6481 ggaaagcgag cccccatcgg ttttcgtatc caccgagccg gccgagctga tcctgattga
     6541 cggcgagccg gaactggtag aaatcggcac ccttggcctg tccttcgtcg cgaataccga
     6601 aagcccgctg ttcggctact ccgagcgctg gtactacctc gtttccggac gctggttcac
     6661 gagcagggcg ctcgatggca ggtggtcggc cgtgctcgag ttgcccaagg cgtttgccca
     6721 gattcctgaa gatcacgaaa aggctgacgt gctggccgcg gttcccgata cgccagcagc
     6781 cagaatggcc gctctggatg cccagattcc cgagaagacc gaggtgcctc gggatctcag
     6841 catgccggaa accgtgacct atgtgggcga accggtgttc gagcccatcc cgggcaccaa
     6901 cctcaagcgt gcgacgaaca ccgcctactc ggtcattcag gccggcgcgg actactacct
     6961 gtgctacagc ggcatgtggt acgcggcttc ggcgcccgaa gggccctggc gcgtgaccag
     7021 tcacgtgccc tcccaggtct accagatccc gcccagctcg ccggtctaca acaccaccta
     7081 cgtgaaggtt caagaggaaa gcccggtctc ggtgaccttc ggcgtgaccc agggctactc
     7141 gagcgtgtac gtctccaacg gggtgcccgt ctacggaacc ggctggtact acccgcccta
     7201 ctactattac tacggcggct atccgatcta cttcgcttat ccgtacacgt atggctccgc
     7261 ctctttctac aacccggcca cgggcaccta cggcagcgtt tcgagggcct acggacctta
     7321 cggcggctgg ggttacgcct cggcctacaa cccgaccacg ggcacttacg gtcgggcgga
     7381 ggcggtttgg gatcacgacg agtggtatgc ggtgggcgag gcctacaacc cccacaccgg
     7441 gcggtactac accaccgagc ggtactacga tgccgatgac gagcgctggg aaatcgactc
     7501 ccggttctcc ggcgagcgtg gttacgtaga cgtgtccaga gagttcgacg aagactccgg
     7561 tcgcgccacc attgaaacgt cccggggcgg ggaaggcacc ttctctcgcg agaaaagcga
     7621 cgggggctgg aacaccagcg gcgagttcga gaccgccggc ggccgcaccg tcaccggcag
     7681 cggccgctac gaagacggcg aaggaagctc cacgctgcgt ggctccgaag ggggtaccgg
     7741 caccatagaa aggtcggtgg atgatggcgt ggtgacccgg gacggcacct tttccaagga
     7801 tggcaagacc atcgagacgg aaaccacccg cgacgggacg gggaggcgca cgtcgttcga
     7861 gacctcagaa ggcaccagcg gcgcggtcgg cggccgggga cagcagcgca cggcgctggc
     7921 gcagaccgag tccggtgatc tgtacgcggc gcgagacggg aacgtctaca ggaaatcgga
     7981 tgacggctgg tcgcgtcaca gcgacggcag ctgggagacc atcccgatgc ccagccgcga
     8041 cgccgatgcc ggcgcccgat cctatggcgg gggcgattac cgccagtccg cgtctagcgc
     8101 ctatcgaggc ggcgcggatc ttgatgccga tcgctatcgc ggcctggatc gcgattactc
     8161 cgcccgacag cgcggcatga cccgcgccgg ctcgtatggg ggacaacgcg gcagcttcgg
     8221 tcggctgcag ggccggcgcc gttagcacca gctggtacgg cctggaaata cggttgctag
     8281 gtgccggatc gggagcggtc caggggcgat tgggatcagc cggattcgaa cgcgctggcc
     8341 gcggcaaact gccaggcgcc attttcctgt cggcaaagat agtggacgga ttgcccctgg
     8401 gtacccttgg cggtcacgtt gcggaacgcc gccttgcgac aggtctcgcc ttccacctcg
     8461 gcggtctcga gaattctgat cgacccgctg ttgccggttt tcgggttgct ccagtcgaag
     8521 cgctcgccgt cagcgccgtc ttccagggcc tggcgcattt ccgctttgaa cagatcccag
     8581 tcgctctcct tgaactcgtt ggcggccgac ccgcgcatcc agccccagaa ggccgtggca
     8641 ggcgccgcaa cgacgaccag caggagcagg gtaaggattc gcgagtgccg ctgcgccatg
     8701 atgttttcga ccccgtcgaa aaaggagtca agtctagcct aaggtcgcgg atctgctcca
     8761 ccgatcccgg cctggcagcc ggcgcagcgc ttccggcggc gagggggtga ggtgactcga
     8821 tgcgccaggt ttagccggtt tgcctgtcca cgagacccgg gtatcatgcg ggcggcgcgc
     8881 gacggaccga gacgaaaatg ctatgtgcgg cgcgctgcag atgcctcgaa gctcgctatg
     8941 ggcgggctca acgaaaatcc atttttgaaa cgctagggag tttacacatg cgacgcattt
     9001 tgctcgcgat gggcgcgtct ttgctcttgc ccgccatggg gctggctgcc gacaagccaa
     9061 acatactcgt catctggggc gacgacatcg gtcaaagcaa catcagtgcc tacacccgcg
     9121 gcatgatggg ctaccccacg cccaacatcg accggatagc ggccgagggt atcctgttca
     9181 ccgactacta cggcgagcag agctgcaccg ccgggcgctc gtccttcatc atgggtcaga
     9241 gcgtcttccg taccgggctg tcgaaggtcg gtctgccggg cgcggaggaa ggcatgcagg
     9301 tggaggaccc ctccatagcc ggcctgctga aggaccaggg ctacgctacg ggccagttcg
     9361 gcaagaacca tctgggtgac cgagacgagc acctgcccac caatcacggt ttcgacgagt
     9421 tcttcggcaa tctgtatcac ctgaacgccg aagaggaacc cgagaacgag gactacccga
     9481 agaatcccga gtttcgcgag cgtttcggcc cgcgcggcgt cattcgctcg agcgccgacg
     9541 gccagatcga ggacaccggg ccgctcacca agaaacgcat ggaaaccgtc gatgacgaaa
     9601 ccgtggcggc ggccatggat ttcatggagc ggcagcatgc cgcgggcaag ccgttcttcg
     9661 tctggtggag cggaacccgc atgcacttcc gcacccacgt caaggacgag atgcgcggta
     9721 tttccgggca ggacgagtac tcggacggca tggtcgagca cgacatgcac gtggggatgt
     9781 tcctggacaa gctggatgcg ctcggcattg ccgacaacac catcatccac tactccacgg
     9841 ataacggccc gcactacaac acctggccgg acgctgcctc tacgcctttc cggggcgaga
     9901 agaacaccaa ctgggaaggc ggctggcgcg tgccggcagc cgtgcgctgg cctggcgtcg
     9961 tgaagcccgg gacggtaagt aacggcatcg ttcatcacat ggactggctg ccgacatttc
    10021 tggcggcggc gggaaagacc gacatcaagg aagaccttct ggacgggtac cgctcccctg
    10081 ccatgaagcg gcgctaccgt gtccatctcg atggctacga cctcaccgac cacctcaagg
    10141 atccccaggg ggtggaaagc ccgcgcaagg agatttttta cttctccgac gatggcgatc
    10201 tgacggctct acgatatcag gactggaagc tgatcttcat ggagcaacgc gtcgaaggca
    10261 cgcttcaggc ctgggccgag cccttcgtgc cgctgcgtgt tccgctgctc ttcaacttga
    10321 ggcgcgatcc ttacgagcgc tcgcagaaga cgtccaacac ctactacgac tggctcattg
    10381 atcgggtctt cctcatggtg ccggcgcagg tctacgtggg ccagttcctg gccacgttca
    10441 gggagtaccc gccgcgccag aaggcggcga gcttctccct ggagcgggtc ctcgagcagc
    10501 tgcaggaggg cggcgactcg cgctagcgcc gttcatcgac ccagtgggcg gccgtattcg
    10561 gcggtcgccc gctgcttctc ctgaagcgcc aggtcctttc cgcagacgct ctcccgttcg
    10621 aaaccggcgg gctacggctc gtctgactcc tggctgctca aatacgtgaa gaaatcccgg
    10681 gcaatcttgg atcgtgccga ccagcctgct tgctccagat ggcttctcgc cagggcagag
    10741 atgtctccgg aaatgtagac ccggccgtcc gaagggacgt cggggtcctg tgccggccac
    10801 aggccgacgg cctcgacggt ggtcgggttc cacgtcaggt ggtcaacggg aagcatggcg
    10861 acacggttgc cgccggtgtc gacggcgatc gagacgggtc cgtgcacggt gatggcagca
    10921 acgttctccc cctcctggtt caaccacgcg atcatggcca ggctctggag gtagaatcgg
    10981 gattcggcct cgtcctcggc cagggtcgcc tgacgcagga aggcgtcccg cccggcagtg
    11041 gtttcgagca gccccagacc gttgatgatc agggtctgaa tggtggggct gatggcgacg
    11101 ttatctaaaa acgcggtcgc ctcctcctcc gccacaccca tgtcctgcag cttgaccagg
    11161 ttcatctggc gcaactcgta ggcgtcgcgt tcccagatgg cctcgttgac gtccaccacc
    11221 agatcgacga tgcctccgat gggcaccgcc agctcggcca ggaaatagcc gctgctgtcg
    11281 acgacggcca cgtcggcgat tccctttctg agagtttcgt tggaggtata tggatcggta
    11341 cccagcttct tggcccagcg ccgctccgcg cgggtggttc ccatcaggct tttgccgaga
    11401 tcgacggccc cctcgcctgc ctgctccgcg atgctggcct cctccccgga gtcgtcggcg
    11461 cccgtcgccg actttgctgc cgacgccgtc gtggccaccg cgccctcgac tttgcgtttc
    11521 tgcctgttga acatgcgtgc caggcccccg gggagcccgg tgacggtgcc gatggggttc
    11581 gtgacgatgg tcgcggcggc gctgacgggg gcggtgacgg cccttaccgc cgactgggca
    11641 gctgcttcca tgaaaacttt ggttttcgac aggccgtcca gctcggccag cgcgcctatc
    11701 tcgttgatgc gcgttcgaag catggacgag ccgtaggctt caaatgtccc gaaaggcgag
    11761 tcgatgacgt aggtgttcat gaagccatcg ttgacgattt cctcgcgtac ctcgtggtgc
    11821 tctccgttgc gggtctcggc gctgagcact tcgctggcct tcagaatcaa gggggaatcg
    11881 aattcctgct gctcctcggt gtcggtggct tgggatggca cgcacaccgc ggctagaatg
    11941 agcgccagcg agacgttacg tatattcata gaaaatcctt gggcgttgtc gagaaccggc
    12001 tctgatgccg gcaggtccct cgccgggaac agcgctgcgg ggtcttcaca tcttagtcgg
    12061 gtggatcgag ggctgcatcc gccagggaaa tcatgtggct cgcgccatcg cgagggctct
    12121 tccgggaaag cttatggtca gtgtcaggcg acgacggggc cggcagggtg gcggacgttg
    12181 ctgcccgagg tgacgattgg atgacgggct tattcctgcg cgcgctgctc gcttggctcg
    12241 ctgtgtgtcc tgcgctggtg gagggggccg aagcggtctc cccggacacg gcgacggcgg
    12301 gcgagcccgg ccggccactg ccgatggaat cggatggaaa gaggaaccct ctgacgcccg
    12361 atgttcgcga cgacgatacc gagctgacga tgcagcgggg cgacttcgtg attgccccca
    12421 ttccgatttc ggacccggtg cttggagagg gtctggttct cgcggccgcc tatttctatc
    12481 cccagacgga ggagcaggca gcttcccagc ctgcttccgt caccgcggtg gcgggaatgt
    12541 ataccagcag cgagagctac gcttacggta tcggccagca gaactactgg ggcgaggaca
    12601 gatggcgctt cagcggcgcc ctggctcatg ccgacttgca gctgaagctt cgccccggcg
    12661 cggagaacgg cgacgggggg cggaccgatt ggctgatcga tggcaacgtc ctgcaggcag
    12721 agctttcacg catgatcgcg ggagactggt acctgggcct gatcagccgg ttcgtggata
    12781 tcaaccagag ctttgaaaca gaccggcaaa ccggaaactt tgacctgggc tcgagtattc
    12841 gcactgtcgg gatgggcctc agcgtggaac acgacagccg ggacctcccg ctgaactcct
    12901 tctcgggccg gctcttcgag ctgcgggggc tgttcaacga ct
//
LOCUS       DBNHLM010000348        12409 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1668689, whole genome shotgun sequence.
ACCESSION   DBNHLM010000348 DBNHLM010000000
VERSION     DBNHLM010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12409
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1668689"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..2065
                     /gene="xsc"
                     /locus_tag="AC16QF_15985"
     CDS             299..2065
                     /gene="xsc"
                     /locus_tag="AC16QF_15985"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: protein motif:HMM:NF005713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HIR6450110.1"
                     /translation="MKMTSEEAFVKVLQRHGIDNAFGIIGSAFMPVSDLFTRAGITFW
                     DCAHEGSGGMMADGFTRASGRMSMMIAQNGPGITNFVTAVKTAYWNHTPVLLVTPQAA
                     NQTIGQGGFQEVEQMKLFEDMVCYQEEVRSASRMAEVLNRVILQAKRHSAPAQINVPR
                     DLFTQVIDIELPPIIDFERPSGGTRAIDEAAALLSKAEFPVILNGAGVILADAIGDSA
                     ALAERLEAPVCCGYQHNDAFPGSHPLFAGPLGYNGSKAGMELIAHADVVLALGTRLNP
                     FSTLPGYGIDYWPKDAAIIQVDINPSRIGLTKPLSVGIVGDAGDVARALLERVEEVSD
                     EERDARRNLIARTKANWQQELAGMDHEDDDPGTTWNERARADKPDWMSPRMAWRAIRS
                     VLPTEAIISSDIGNNGAIGNAYPTFETGRKYLAPGLFGPCGYGLPSIIGAKIGCPDVP
                     VVGFAGDGAFGISVTELTAIGREEWPAITMIVFRNYQWGAEKRNSTLWFDDNFVGTEL
                     DEQVSYAGIAQACGLLGVQARTMDELSAALGEALDNQMNHGRSTLIEAMINQELGEPF
                     RRDAMKKPVEVAGIDPADMRRA"
     gene            complement(2105..2689)
                     /locus_tag="AC16QF_15990"
     CDS             complement(2105..2689)
                     /locus_tag="AC16QF_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450111.1"
                     /translation="MGVFEYVAGFVAIVVGLAVARVMGGIGAFILSDRRAAMDWLLAG
                     WCLALIVTLIGWWMLAWFTLGQLETITYGAVFGWFAATALLYLASYILVPAGGAAFGG
                     AGAALGELRPAFFVCLAVHFAANIVESAVTGSLLPAQGLVVLMVVLSGIGAFLRGARA
                     HALLLLTWMVCMLVLNFVAVPAVGVNSGSVLEAG"
     gene            2835..3539
                     /locus_tag="AC16QF_15995"
     CDS             2835..3539
                     /locus_tag="AC16QF_15995"
                     /inference="COORDINATES: protein motif:HMM:NF013223.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriorhodopsin"
                     /protein_id="HIR6450112.1"
                     /translation="MDKLFLQSGDFVGVTFWLVSIAMIAATVFFLYEGVKVKPQWRLS
                     LLVAGLVTLVAGVHYDYMRDYWVVEQATPIVYRYIDWLITVPLLMIEFYIILRAAGAA
                     LGHGSFVRLLVGTLVMLIFGYLGEAQVLNIHVGFWIGMVGWAIIIYEIFGGAAGKAAS
                     ASAQAVQSAFSAMRLIVLVGWAIYPIGYIWGYMLGGVDEGALNMVYNLADFVNKILFG
                     LIIWNLAIKDSGEAGH"
     gene            complement(3601..4122)
                     /locus_tag="AC16QF_16000"
     CDS             complement(3601..4122)
                     /locus_tag="AC16QF_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450113.1"
                     /translation="MSIVELGAIGEFLGAFAVVITLVYLGLQVRHNTTSTRAGTLAYA
                     TDAWSDYLQRQSVDDLNLLLGLSIEPDELSSAEFMRAYYLYRVMFRRMESDFYQYRSG
                     ILDADTWDAYVESWRGDVFITPGGRAMWKLQRDYFGPEFRSAFDDVEASTPRTPQHLR
                     RRFAELWEAERPV"
     gene            4278..4790
                     /locus_tag="AC16QF_16005"
     CDS             4278..4790
                     /locus_tag="AC16QF_16005"
                     /inference="COORDINATES: protein motif:HMM:NF016162.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HIR6450114.1"
                     /translation="MWKFTGKSRPEFAEEPGPGQESVWDYPRPPALDPSDEQVEVRAD
                     GTPIASSRRTLRVLETAGPPTYYIPEQDIDWSQLTEIAQTTYCEWKGHASYYALASDP
                     ARVPVAWLYADPSDSFRAIDRHASFYPGRVECLVNGERVRPQPGEFYGGWITDRVVGP
                     FKGEPGTGHW"
     gene            complement(4806..5780)
                     /gene="egtD"
                     /locus_tag="AC16QF_16010"
     CDS             complement(4806..5780)
                     /gene="egtD"
                     /locus_tag="AC16QF_16010"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052706 - L-histidine
                     N(alpha)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HIR6450115.1"
                     /translation="MKQARRKARNVMFRDEHPDLGDSRREILAGLQRDQKAVNPKWFY
                     DERGSQLFDQITRLPEYYPTRTEVGILQENRREISACCGSGCLFIEPGSGSSEKVRIL
                     LETLKPSAYVPVDISAGFLEEAALKLGDEYPWLDIYAVCADFNRSWSYVDDLPEGKRV
                     VFYPGSTIGNLEPEAAVGFLRRVREITGDSGGVVIGVDTHKSTERLNAAYNDAAGVTA
                     DFNLNVLRRMNDLLDAEFDEGSFRHHAFYNTELKRIEMHLVSERAQAVRCNGATVEFA
                     EGETIHTENSYKYTVEDFAELVDAAGLELQQSWTDADELFSVHYLRVA"
     gene            complement(5777..7051)
                     /gene="egtB"
                     /locus_tag="AC16QF_16015"
     CDS             complement(5777..7051)
                     /gene="egtB"
                     /locus_tag="AC16QF_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HIR6450116.1"
                     /translation="MGKALAIDATAAAFSDTRRQSVDVCRPLEIEDYGLQAAVFASPP
                     KWHLAHTTWFFETFILKPFVSGYAPVEPIYEVLFNSYYNGIGEQHPRHQRGLLSRPTL
                     DEVLAYREHVDAAMAGCLELAGRADPQAIRQRTVLGIEHERQHQELFFTDLKYSLSVN
                     PLYPAYLAAELPVQSGTPVRWLGCEGGLVEIGHDGSGFCFDNELPRHRCFVEPFEIAS
                     RLVTNEEYQAFVDDDGYGRPELWLADGWTTLQSEGWKAPLHWLERDGQALEFTLHGLQ
                     PRRGSRPVCHVSGYEADAFARWANARLPTEQEWETVAAGHPAGAAGMDDGVYHPQAAP
                     ADEGPTQLYGACWQWTRSAYGPYPGYSASPGAIGEYNGKFMSNQWVLRGGSCVTKSSQ
                     ARPTYRNFFYPQDRWQFSGIRLARSVEGERRL"
     gene            complement(7193..8668)
                     /locus_tag="AC16QF_16020"
     CDS             complement(7193..8668)
                     /locus_tag="AC16QF_16020"
                     /inference="COORDINATES: protein motif:HMM:NF015995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR6450117.1"
                     /translation="MKGACLLLAAACIVLVAPVRAAERVTVASKAFSESYVLAEIAAQ
                     LLEAEGFEVERKLGLGGTLIAFGALREGAVDVYPEYTGTLTQAILDEPGMTIAELDAA
                     LAERGLVFRVPLGFNNSYAVAVTEEMAGARGLTRVSDLAGQTDLSFGFSHEFLNRADG
                     CPALKTAYGLTQTASGIEHALAYRALASGHIDVTDAYTTDGELSLYALRLLADDRGFF
                     PTYDAGLLTRDDMPAPVAAVLSRLESRIDEETMQGLNRRVASQGEEPARVAADYLRET
                     GLVSAADAVAVAGYERILGNTLVHLKLTGIALLLGCLVAVPLALVVARHQRIASGLLY
                     VTGLVQTVPSLALLALLIPLVGLGQLPAIIALFLYSLLPIVRNTLTGLFNVDPLLKEV
                     AGSMGLTTAQQLRKVELPLAMPTILAGIKTAAIISIGTATLAAFVGAGGLGEPIITGL
                     TLNDHALILEGAVPAALLAVVVELLFELIERATVPAHLRVS"
     gene            complement(8665..9405)
                     /locus_tag="AC16QF_16025"
     CDS             complement(8665..9405)
                     /locus_tag="AC16QF_16025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6450118.1"
                     /translation="MPVLSLQDVTKTYGRRPVLENVDLALESGQVTAIVGRSGCGKST
                     LLKLFNGLVVPDRGRVSAFGAELDYADLVSVRRRMGYAVQGTGLFPHMSARENVVLSA
                     EIAGWKEQAVAQRLGELLELTRLSAELLAKYPHELSGGQQQRVGLCRAMMLRPEVLLL
                     DEPFAAIDPITRYDIHHQLLEILAAEPATVVLVTHDMREAMQLADRIVLLEGGRVFLD
                     ESRDSLARSRPGVEPEALLQSLMEEALA"
     gene            complement(9508..10059)
                     /locus_tag="AC16QF_16030"
     CDS             complement(9508..10059)
                     /locus_tag="AC16QF_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450119.1"
                     /translation="MMRPAMTRIAALVVLFAPIASLQADHHGALIAQARSAAPELVSA
                     DATVMYRGEVLVEGTNGWVCMPETLPDDNSPMCNDAVWMGLMQAMDAGEEYEVERIGI
                     SYMLQGDAGVSNSNPAHADHVNAPDFIKEGAHLMIAVPRAMLEGITDDPHGGGPYVMW
                     KDTPYAHIMVPLEDANRSAPPAQ"
     gene            10221..10622
                     /locus_tag="AC16QF_16035"
     CDS             10221..10622
                     /locus_tag="AC16QF_16035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6450120.1"
                     /translation="MATFLAKIKIFEGKEAEFEETARMMFRETHANEPTCRRYEYWRG
                     AEPRTYYCMESFDDFVGFMEHQVSPHHEAPDFASMLEEIELEWIDPIQGACDLTSTNP
                     KDLPEDASDLMKEYAATHPLVMQDWWLNLRK"
     gene            10635..11744
                     /locus_tag="AC16QF_16040"
     CDS             10635..11744
                     /locus_tag="AC16QF_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HIR6450121.1"
                     /translation="MKTRAAVAFEPKKPLEIVEVDLEGPKAGEVLIEIKATGICHTDA
                     YTLDGLDSEGVFPSILGHEGAGVVVETGPDVTSVAPGDHVIPLYTPECRQCKSCLSRK
                     TNLCTAIRATQGQGLMPDGTSRFSFEGKPIHHYMGCSTFSNYTVVPEIAVAKIRADAP
                     FDKACYVGCGVTTGVGAVVNTAKVEAGANCIVFGLGGIGLNVIQGLRMVGASRIIGVD
                     VNPRKEAWGRQFGMTHFVDPGTVDGDLVAHLVELTDGGADYTFDCTGNTDVMRTALEA
                     CHRGWGTSVIIGVAEAGREIATRPFQLVTGRVWKGTAFGGARGRTDTPKVVDWYMDGK
                     IEIDPMITHTLPLERINEAFDLMHRGESIRSVVVY"
     gene            11909..12346
                     /locus_tag="AC16QF_16045"
     CDS             11909..12346
                     /locus_tag="AC16QF_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6450122.1"
                     /translation="MTANSPTGDYVQMDSVDWIPFPEGLCEGPITWKLLHVSPESGAW
                     TAIFDCPAGTSFAKHIHMGPGEYFLTKGRMNVRGGSAEGGATAVAPGYGYEECNAQHD
                     HTEFPVDSEFYMTFLGPLNFIDDDGNTIALVGCKEVLDAWNAT"
BASE COUNT         2323 a         4126 c         3918 g         2042 t
ORIGIN      
        1 ggcaatctgc tcatgtatca gggcatgacg gagatgagct tctgccaggc ttgggcaaag
       61 ccgcgcaccg tcaacgggct catggtgtgg ccgccacacc aactgctccc ccaatcggtc
      121 gacaggcacg gccgcccctg ccggtaagcg acgaccggac tttcggcaac ccgctggaaa
      181 agtccgtggt cccgtgttaa acacgcccgc tggctctgcg gacaccgagc cgaacgggac
      241 ttgaatcacc atcgagcagc tgctcgcgcg acggcgcggg cgtggagatt tgtcgaccat
      301 gaaaatgacg tctgaagaag ccttcgtgaa agtcctgcaa cggcacggca tcgacaacgc
      361 gttcggcatc atcggatccg ccttcatgcc cgtctcggat ctcttcaccc gggccgggat
      421 taccttctgg gactgcgcgc acgaaggatc cggcggcatg atggccgacg ggttcacccg
      481 ggcctcgggc cggatgagca tgatgatcgc ccagaacggt cccggcatta cgaacttcgt
      541 gaccgcggtc aagaccgcct actggaacca cacccctgtg ctcctggtga cccctcaggc
      601 cgccaaccag accatcggtc agggtgggtt ccaggaagtg gagcagatga agctgttcga
      661 ggacatggtc tgctaccagg aggaggtgcg ctccgcctcc cgcatggccg aggtgctcaa
      721 ccgcgtgatc ctgcaggcca agcgacactc ggcacccgcc cagatcaacg tgccgcggga
      781 cctgttcacc caggtgatcg acatcgagct gccgccgatc atcgacttcg agcgcccgag
      841 cggcggtacg cgggccattg acgaggccgc cgccctgctg tcgaaagccg agttccccgt
      901 catcctgaac ggcgccggcg tgatccttgc cgatgccatc ggcgattccg ccgccctggc
      961 cgagcgcctg gaggctcccg tgtgctgtgg ataccagcac aacgatgcct ttcccggctc
     1021 gcaccccctg ttcgcgggcc cgctggggta caacggatcc aaggccggca tggagctcat
     1081 tgcccacgcc gacgtggtgc tcgccctggg gacgaggctc aacccgttct cgaccctgcc
     1141 cggctacggc atcgactact ggccgaagga tgccgccatc attcaggtcg acatcaatcc
     1201 gtcacgcatc ggcctcacca agcccctgag cgtaggcatc gtgggtgatg cgggcgacgt
     1261 cgcccgggcc ctgctggaac gcgtggagga ggtgagcgac gaagagcgtg acgcgcggcg
     1321 caacctgatc gccagaacca aggccaactg gcagcaggag ctggccggca tggatcacga
     1381 ggacgacgat ccgggaacca cctggaacga gcgcgcgcgc gccgacaagc cggactggat
     1441 gagcccccgc atggcctggc gggcgatacg cagcgtgctg cccacggaag ccatcatcag
     1501 ctcggacatc ggcaacaacg gcgccatcgg caacgcctac cccaccttcg agaccggcag
     1561 aaagtatctt gcgcccggcc tgttcggccc ctgcggctac ggcctgcctt cgatcatcgg
     1621 cgcgaagatc ggctgccccg acgtgccggt ggtgggcttc gccggcgacg gcgctttcgg
     1681 catctcggtc accgagctca ccgccatcgg ccgcgaggag tggccggcca tcaccatgat
     1741 cgtcttccgc aactaccagt ggggggcgga gaaacgcaac tccacgctgt ggttcgacga
     1801 caacttcgtc ggcacggagc tcgacgagca ggtgagctac gccggcatcg cccaggcctg
     1861 cgggctgctc ggagtccaag ccagaaccat ggacgagctt tccgccgctc tcggtgaagc
     1921 gctggacaac cagatgaacc acggcaggtc gacgctcatc gaggccatga tcaaccagga
     1981 gctcggcgag cccttccggc gcgacgccat gaagaagccg gttgaggtcg caggcatcga
     2041 tcccgcggac atgcgacgcg cttaggctgg aatcggggct ggccgggcag cgggcgcgtt
     2101 cggctcagcc tgcttccagc accgagcccg agtttacccc cacggccggg accgcaacga
     2161 agttcaggac aagcatgcag accatccagg tcagcagcag cagggcgtgt gcccgggcgc
     2221 cgcgaagaaa cgcgccgatt cccgagagca cgaccatcaa cacgaccagc ccctgagccg
     2281 gcaagagaga acctgtaaca gcggactcga cgatgttggc agcaaagtgc acggccaggc
     2341 agacgaagaa ggcgggccgc aattcgccca gggccgcacc ggctccgccg aaagccgctc
     2401 ctccagcggg aaccagaatg tagctcgcca gatagagcag cgccgtcgcc gcgaaccaac
     2461 caaatacggc gccgtaggtg atggtctcca gctgccccag ggtgaaccag gccagcatcc
     2521 accatccgat cagcgtgacg atcagcgcca ggcaccagcc ggccaggagc cagtccatcg
     2581 ccgcgcgcct atccgacaga atgaaagcgc cgatcccacc catgacccgc gcgacggcga
     2641 ggccgacgac gatggcaacg aacccggcta cgtattcgaa cacgcccatg gcaactcccc
     2701 gtcgcacacc ctggcctttc ccgatccagc atcaccagtg atgacagacc cgttcacgca
     2761 gctttcacgt gagccctgtt gacatccacg atgcagtaaa actcaagcaa taacgcaatt
     2821 tacagggatt aacaatggac aagctctttc tgcagagcgg cgacttcgta ggcgtcacct
     2881 tctggctggt gtctatcgcg atgatcgctg ccaccgtctt cttcctttac gagggcgtca
     2941 aggtcaaacc gcagtggcgg ctctcacttc tggtcgccgg cctcgtgact ctggtcgccg
     3001 gcgttcacta cgactacatg cgcgactact gggtcgtcga gcaggcgacg cccatcgtct
     3061 accgctacat cgactggctg atcaccgttc cgctgctgat gatcgagttc tacatcatcc
     3121 tgcgcgcggc gggtgcagcc ctgggacacg ggtcgttcgt gcggcttctc gtcggcacgc
     3181 tggtgatgct catcttcggc tatctcggcg aagcccaggt gctcaacatt cacgtcggct
     3241 tctggatcgg catggtcggg tgggccatca tcatctacga gatattcggc ggagctgccg
     3301 gcaaagcggc atctgcctcg gcgcaggcgg tgcagtccgc tttcagcgcc atgcgcctga
     3361 tcgtgctggt tggatgggcc atctacccga tcggctacat atggggctac atgctcggcg
     3421 gcgtggacga aggcgcgctg aacatggtgt acaacctggc cgacttcgtg aacaagatcc
     3481 tgttcggtct gatcatctgg aacctcgcca tcaaggactc gggcgaagcc ggacactgag
     3541 ttagcccagc cacctgaggg ccccacccgg ggtggggccc gttcggctga gagcagaagg
     3601 tcaaaccggc cgctcagcct cccacaactc cgcgaaccgg cggcgcaggt gctgaggcgt
     3661 gcggggcgtg ctcgcttcga cgtcatcgaa agcgcttcga aactccggtc caaaataatc
     3721 gcgctgcaat ttccacatgg cgcgtccgcc cggcgtgatg aacacgtcgc cgcgccagct
     3781 ctccacgtaa gcatcccatg tgtccgcatc gaggattccg ctgcgatact gatagaagtc
     3841 gctttccatc cgccggaaca tcacgcgata cagatagtag gcgcgcatga actccgcgct
     3901 ggacagctcg tcgggctcga tcgaaagccc cagcagcagg ttcagatcgt ccaccgactg
     3961 tctctgcagg tagtcgctcc aggcgtccgt cgcgtaggcg agcgtgcccg cccgggtcga
     4021 cgtggtgttg tgacggacct gcagacccag gtagaccaga gtgatgacca cggcaaacgc
     4081 gcccagaaac tccccaatcg cgccaagttc tacgatgctc atgaatggct cctcaattac
     4141 ggcatgccaa gcgtaacgaa gctcaggcgt ttggccgagt cccgatctcc agggaagcac
     4201 ggcatcgacc acgaaccgcc gtcgacgggc tagcgcccca tgccggccgg gttgaacacc
     4261 tgttagtatc ctcggccatg tggaaattta cgggaaagag cagaccggag tttgccgagg
     4321 aaccgggacc cggccaggag tccgtctggg actaccctcg cccaccagcg ctggacccga
     4381 gcgacgagca ggtcgaggtg agggctgacg ggacgccgat cgcatcaagc cgcaggacgc
     4441 tgcgggttct ggagacggcg gggccgccca cctattacat cccagagcaa gacatcgact
     4501 ggagtcagct gacggagatc gcccaaacca cctactgcga gtggaagggt cacgccagct
     4561 attacgctct cgcatccgac cccgccaggg tgcccgtcgc gtggctttac gccgaccctt
     4621 ccgactcctt ccgggccatc gaccggcacg cctcgttcta tccggggcgg gtcgagtgct
     4681 tggtcaacgg cgagcgggta aggccccagc ccggggagtt ctacgggggc tggatcaccg
     4741 accgcgtggt cggcccgttc aagggagaac ccggcaccgg gcactggtag acctcgggct
     4801 tcgggtcagg ccactctcag gtagtgaacg ctgaagagct cgtcggcgtc cgtccagctc
     4861 tgctgcagct ccaggccggc cgcgtccacg agctccgcaa agtcctccac ggtgtacttg
     4921 tacgagttct ccgtgtggat ggtctctccc tcagcaaact cgactgtggc accgttgcag
     4981 cgcacggctt gggcccgctc gctcaccagg tgcatctcga tccgcttcag ctcggtgttg
     5041 tagaacgcat ggtggcgaaa gctcccctcg tcgaactcgg catcgagcag gtcgttcatc
     5101 cgccgcagca cgttcaggtt gaagtccgcc gtgacgccgg ccgcatcgtt gtacgccgcg
     5161 ttcagtcgtt cggttgactt gtgcgtgtcc acaccgatga cgacgccgcc cgaatcgccc
     5221 gtgatctcgc gaacccgacg cagaaacccc accgccgcct cgggttcaag gttgccgatg
     5281 gtcgacccag ggtagaaaac cacccgttta ccttccggca ggtcgtccac gtaggaccag
     5341 ctccgattga aatccgcgca gaccgcgtag atgtccagcc aggggtactc gtcgccgagc
     5401 ttcaaggcgg cttcctcgag gaaaccggcg gagatgtcca cggggacgta ggctgacggt
     5461 ttgagagtct cgagcaggat cctgaccttc tcgctggaac cgctcccagg ctcgatgaac
     5521 agacaaccgc tgccgcagca ggcgctgatc tcgcgacgat tctcctgcag gatccccacc
     5581 tccgtccggg tcgggtagta ctcgggcagg cgggtaatct gatcgaacag ctgggagccc
     5641 cgctcgtcgt agaaccactt cgggttgacc gctttctgat ctctctggag accggccaat
     5701 atctcgcggc ggctgtcgcc gagatccgga tgctcgtccc ggaacatgac gtttcgagcc
     5761 tttctgcgcg cctgcttcat aaccgtcgtt cgccctccac ggagcgggcc aggcggatgc
     5821 cgctgaactg ccagcgatcc tggggataga aaaaattgcg gtaagttggc ctcgcctggc
     5881 tggacttggt cacgcaggag cctcctcgca gaacccactg attgctcatg aacttgccgt
     5941 tgtactcgcc gatggcgccg ggcgaagcgc tgtaacccgg gtacggaccg tacgcgctcc
     6001 gcgtccactg ccagcacgcg ccgtacagct gcgtcgggcc ttcatctgca ggagctgcct
     6061 gagggtggta gacgccgtcg tccatgcccg ccgctccggc gggatgtccg gccgccaccg
     6121 tttcccactc ctgctcggtc ggcagccgcg cgttcgccca gcgggcgaaa gcatcggcct
     6181 cgtagccgct cacatgacag acgggccgcg acccccgcct cggctgcagg ccgtgcaggg
     6241 tgaactccag cgcctggccg tcacgttcga gccaatgcag cggcgctttc catccttcac
     6301 tctgcaacgt cgtccagccg tcggccagcc acagctccgg acgcccgtag ccgtcgtcgt
     6361 ccacgaacgc ctggtactcc tcgttggtga cgagcctgga ggcgatctcg aaaggctcca
     6421 cgaaacatcg atgtcgaggc agctcgttgt cgaagcagaa gccgcttccg tcatggccga
     6481 tctcgacgag gccgccctcg cagccaagcc accgcaccgg cgtcccgctc tgcaccggca
     6541 gctcggcggc gaggtaggcc gggtacagcg ggttgacgct cagcgagtat ttgaggtccg
     6601 tgaagaagag ctcctggtgc tggcgctcgt gctcgatgcc cagaacggtg cgctggcgta
     6661 tggcctgcgg atcggcccgt ccggccagtt ccagacagcc tgccatcgct gcgtccacgt
     6721 gctcccggta ggcgagcacc tcatccagcg tggggcgcga tagcaacccc cgctgatgcc
     6781 gcggatgctg ctcgccgatc ccgttgtagt aagagttgaa gagcacctcg tagatgggct
     6841 cgacgggcgc gtagcccgag acgaacggct tgaggatgaa ggtctcgaaa aaccaggtgg
     6901 tatgagcgag atgccacttc ggagggcttg cgaacacggc ggcctgcagg ccgtagtcct
     6961 cgatctccaa cggccggcag acgtccacgg attgtcgccg ggtatcgctg aacgcggccg
     7021 ccgtcgcgtc gattgccaaa gcctttccca tattgatcgt ctgcccctcc cggcccgcag
     7081 atccgagccg cgcgccgctg agcgtgcgcc attccggggg gtcaggccat aggcccgccg
     7141 cctccgactc ctctaaagcc taacagcttc ggaccggact ttgacgacat cgtcaggaaa
     7201 cccgcagatg tgcgggaacc gtcgcccgct cgatgagctc gaagagcagc tccacgacga
     7261 cggccagcaa agcggcagga accgcgcctt ccagaatcag cgcgtgatcg ttcagcgtga
     7321 gaccggtgat gatcggctcg ccgagcccgc cggcgcctac gaaggcggcc agggtggccg
     7381 tcccgatgct gatgatcgca gcggtcttga tgcccgcgag gatggtgggc atggccaggg
     7441 gcagctccac cttcctgagc tgctgcgccg tggtcagccc catgctcccg gccacctcct
     7501 tgagcagcgg gtcgacgttg aacagcccgg tgagcgtgtt gcgcacgatg ggcagcaggg
     7561 agtagagaaa cagcgcgatg atggccggca gctggcccag gccaacgagc gggatgagca
     7621 acgccagcag cgcaagggaa ggcacggtct gcaccaggcc cgtcacgtac agcaggccgc
     7681 tggcgatgcg ctggtgccgc gcgacgacaa gggccagggg cacggccacg aggcaaccca
     7741 aaagcagcgc aatgccggtg agcttcaggt gaacgagggt gttgcccagt atccgctcgt
     7801 acccggccac ggccacggca tcagccgccg acaccagccc cgtttcccgg aggtagtcgg
     7861 ccgctacgcg ggcaggctcc tcgccctggc tggccacgcg ccggttcagc ccctgcatgg
     7921 tctcctcgtc gatacggctt tcaagccgcg acagcacggc ggccacgggc gcgggcatgt
     7981 cgtcacgggt cagcagcccg gcgtcgtagg tgggaaagaa gccgcggtcg tctgccagca
     8041 ggcgaagcgc gtagagcgaa agctccccat ccgtggtgta ggcatcggtc acgtcgatat
     8101 gcccgctcgc cagggcgcgg taggccaggg cgtgctcgat tccggatgcc gtctgggtca
     8161 ggccgtaggc cgtcttgagc gcaggacagc cgtcggcgcg attgagaaac tcgtgggaga
     8221 agccgaagct gagatccgtc tgcccggcca ggtcgctcac ccgcgttaag ccccgggcac
     8281 cggccatctc ttcggtgacg gccacggcgt agctgttgtt gaacccgaga gggacccgga
     8341 aaacgagccc gcgctcggcc agagcggcgt cgagctccgc aatggtcatg cccggctcgt
     8401 cgagtatcgc ctgggtcagg gtacccgtgt actcgggata tacgtccacg gcgccctccc
     8461 gaagcgcgcc gaaggcaatc agcgtgccgc caaggcccag cttgcgttcc acctcgaagc
     8521 cttccgcctc cagcagctgg gcggcgatct ccgcgagcac gtagctttcg ctgaacgctt
     8581 tggatgccac ggtgacccgc tcggccgctc gcacaggtgc cacaaggacg atgcaggcag
     8641 ccgcgaggag caggcatgct cccttcatgc cagcgcctcc tccatcagcg actgcagcaa
     8701 agcctcgggc tccacgcccg gcctggagcg ggcaagggag tcccgggact catcgagaaa
     8761 cacccggcct ccttccagca ggacgatgcg gtccgccagc tgcatggctt cccgcatgtc
     8821 gtgggtgacc agaacgacgg tggcgggctc cgcggcgaga atctccagca gctgatggtg
     8881 gatgtcgtag cgggtgatgg gatcgatggc cgcaaacggc tcatccagca gcagaacctc
     8941 gggcctgagc atcatggccc ggcacaggcc cacgcgctgc tgctgcccgc cactgagctc
     9001 gtgggggtac ttggcgagga gctcagcgga aaggcgcgta agctcgagca gctcgccaag
     9061 ccgttgagcc accgcctgct ctttccagcc cgcaatctcc gccgagagaa cgacgttttc
     9121 ccgggcactc atgtggggaa agaggccggt gccctgcacg gcgtaaccca tccgccggcg
     9181 cacggatacc agatcggcgt aatccagctc cgctccgaac gccgagaccc taccgcggtc
     9241 gggcaccacc agcccgttga acagcttgag cagggtggac ttgccgcacc cgctgcgccc
     9301 gacgatggcc gtcacctggc ccgactccag cgcgagatcg acgttctcga gcaccggacg
     9361 ccggccgtag gttttggtca catcttggag tgacagcact ggcatggcgc tatctaaaca
     9421 tgtcgacggc gccggatgaa aatctcaacg gcaatggacg ccggccgggg ccggcatcag
     9481 ccccgccggg cgctggcacc gggcgtccta ttgcgccggg ggagccgacc ggtttgcgtc
     9541 ctccagcggc accatgatgt gcgcgtaggg cgtgtccttc cacatcacgt agggcccacc
     9601 cccgtgcggg tcatcggtaa tgccctcgag catcgcgcgc gggacggcaa tcatgagatg
     9661 cgcgccttcc ttgatgaaat caggggcatt cacatggtcg gcatgcgcag ggttcgagtt
     9721 gctcacgcca gcatctccct ggagcatgta ggagatgccg atgcgctcca cctcgtattc
     9781 ttcaccagcg tccattgcct gcatcaggcc catccagacc gcatcgttgc acatgggcga
     9841 gttgtcgtcc ggcagcgtct ccggcatgca gacccagccg ttggtccctt cgaccagcac
     9901 ctcgccgcga tacatcacag tcgcatcggc gctcaccagt tcgggggccg ccgaccgggc
     9961 ctgggcgatg agcgccccgt ggtggtccgc ctgcagcgac gcgatcggcg caaacaacac
    10021 aaccagggca gcgatccggg tcatagctgg tctcatcatc tgtctccttg gaaaatcgac
    10081 gctccagacg ttacgcccgg cggcaccctg gggcaaggcg aatcacccag ttgcccatgt
    10141 ccccggtcgc caaacgagga agggtgcggt tatccttgct agaccgataa atcatcatcc
    10201 agggagcatg aggggggacc atggccacgt ttctggcaaa gattaaaatt ttcgagggca
    10261 aggaggctga gttcgaggag accgcgcgga tgatgttccg cgagacccat gccaacgagc
    10321 ccacctgccg acgttacgag tactggcggg gcgcggagcc gcgcacgtac tactgcatgg
    10381 agtcgttcga cgacttcgtc ggcttcatgg agcatcaggt ttcgccccac cacgaagcac
    10441 cggactttgc ctcgatgctc gaggagatcg agctggagtg gatcgatccc atccagggcg
    10501 cctgcgacct gacgtccacc aatcccaaag acctgcccga ggatgccagc gatctcatga
    10561 aagagtatgc ggcaacccac cccctggtca tgcaggactg gtggctgaac ctccgcaagt
    10621 aacagggagc aaatatgaag acgcgtgcgg ccgtggcctt cgagcccaag aagcccctcg
    10681 agatcgtcga ggtcgatctc gaggggccga aggcgggtga ggtgctcatc gagatcaagg
    10741 ccaccggcat ctgccacacg gacgcctaca ccctcgatgg gctcgattcc gaaggggtct
    10801 ttccgtccat cctcggccac gagggcgccg gcgtcgtggt cgagacaggc ccggatgtca
    10861 cttcggttgc gcccggcgat cacgtcattc ccctgtacac gccggagtgc cggcagtgca
    10921 aatcctgtct gtcccgaaag acgaatctgt gcacggccat ccgcgccact cagggccaag
    10981 gcctgatgcc cgacggcacc tcccgattct cgttcgaggg caagcccatc catcactaca
    11041 tgggctgctc gacgttctcc aactacacgg tggtccccga gatcgcggtg gcgaagatcc
    11101 gcgccgacgc ccccttcgac aaggcctgct acgtgggctg cggcgtcacc accggggtgg
    11161 gggccgtggt gaacacggcc aaggtagagg ccggcgccaa ctgcatcgtc ttcggcctgg
    11221 gcggcatcgg gctgaacgtc atccagggcc tgcgcatggt gggcgccagc cggatcatcg
    11281 gggtggacgt caacccgcgc aaggaggcgt ggggacgcca gttcggcatg acgcacttcg
    11341 tggacccggg gacggtggac ggcgatctgg tcgcgcacct ggtggagctc accgacggcg
    11401 gcgcggacta caccttcgac tgcacgggca acacggacgt catgcgcacg gcgctggaag
    11461 cctgccaccg gggctgggga accagcgtaa tcatcggggt ggccgaagcg ggcagggaga
    11521 tcgccacgcg tccgttccag ctggtcaccg ggcgggtctg gaaaggcacc gccttcggcg
    11581 gcgccagagg gcggaccgac acgccgaagg tggtggactg gtacatggac ggcaagatcg
    11641 agatcgatcc catgatcacc cacacgctgc cgctcgagcg catcaacgag gcgttcgacc
    11701 tgatgcaccg gggtgaaagc atccgctccg tggtcgttta ctgaccggac agctctgatc
    11761 gcgaagggag acgggcgccg ccaacgcggt gctaaagcat tgacgcccac ctgagcgcgg
    11821 gacaaactgc agtggagggt gtccgagtct tcatcggacg agtcagcaaa gaacgcagaa
    11881 gctctgcgtt caggttggga ggatcgacat gacagcaaac agccccactg gggattacgt
    11941 ccagatggat tccgtggact ggattccttt tccggaaggc ctttgcgaag gccccatcac
    12001 ctggaaactg ctccacgtct caccggaatc cggcgcgtgg acggccatct tcgactgccc
    12061 cgcaggcacg tcgttcgcca agcacattca catgggcccc ggcgagtact tcctcaccaa
    12121 gggcaggatg aacgtgcgcg gaggctcggc cgaaggcggt gccactgccg ttgcgcccgg
    12181 ctacggctac gaggagtgca acgctcagca cgaccacacc gagttccccg tggacagcga
    12241 gttctacatg acctttctcg ggccactcaa cttcatcgac gacgatggca acaccatcgc
    12301 gctggtgggg tgcaaagagg ttctggacgc ctggaacgcc acttagtcat cattgcccgg
    12361 ccccggcgcc gggcaaaccg ctggaactag ccgggcgctc gtgtgatta
//
LOCUS       DBNHLM010000349         3521 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_890825, whole genome shotgun sequence.
ACCESSION   DBNHLM010000349 DBNHLM010000000
VERSION     DBNHLM010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_890825"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="AC16QF_16050"
     CDS             complement(<1..321)
                     /locus_tag="AC16QF_16050"
                     /inference="COORDINATES: protein motif:HMM:NF017505.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HIR6450123.1"
                     /translation="MKTIRKMLAQLAAGALLAMPVWADETCMSPYMAKIVGQEDFVYV
                     WTLGMEGVGDGQDKLVTVDVNPTSKTYGEVVHSLSVGGRNEAHHSGLTDDRRFLWASG
                     LDTSK"
     gene            390..1619
                     /locus_tag="AC16QF_16055"
     CDS             390..1619
                     /locus_tag="AC16QF_16055"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR6450124.1"
                     /translation="MWRGPITSQAVAALMLTACCTWAVTPGDPRDGYEHADYETASRQ
                     LSALRGSALALAELAGNPPLGLPPLPAERLPVAAAIDLGRRLFFDRRLSANATLSCAM
                     CHVPEQGFTQNELKTPVGIEGRFVHRNAPSLYNVAYQEALFHDGREDDLANQIWAPLL
                     AANEMGNASREAVVERLGGLEEYEGEFLRVFGDGISAATLGRALASYQTALLSADSPF
                     DRWYFGGEADAVGAEVKRGFRSFVNEGCATCHQLQTGHATFTDGAYHDTGIGFLAAAS
                     AGTPARRLQIAPGVIIDVETDIATPHVEDDGRAAVTGDPADRWRYRTPGLRNVALSAP
                     YMHDGSLATLEAVIDYYAAGGAPHDGQDARIRPLSLGADERADLVAFLQALTGSNVDA
                     LAGDARAAPIGDTHHGP"
     gene            complement(1635..>3521)
                     /locus_tag="AC16QF_16060"
     CDS             complement(1635..>3521)
                     /locus_tag="AC16QF_16060"
                     /inference="COORDINATES: protein motif:HMM:NF012361.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR6450125.1"
                     /translation="FVDKLVKNNQQIRWVPDHIKTGRFGNWLEGAIDWSISRNRVWGT
                     PIPIWINDVTGNEVCIGSIDELEALTGVRVDDLHREHVDPLTFTLQGEEGTYHRIEEV
                     LDCWFESGSMPYAQLHYPFENEKVFKAGFPAEFIAEGLDQTRGWFYTLTVLAAALYDK
                     PAFRNVIVNGMVMAEDGKKMSKRLRNYTPPDELMETYGADALRLYLINSGLMRGEEQR
                     FADAGVRDMTRRALLPWYNAFTFLKTYAEIDQWSPDKGQHTGDNVLDHWILSRLQTLK
                     ANVGREMEVYKLYNVVPQLFDFIEDLTNWYIRLNRQRFWGEDITADKIAAYSTLYAAL
                     YELCQVMAPFAPFLAEHVYQELSALGGGRAKPESVHLCDYPEPEGALIRSELEEAVDR
                     MQQVVLLGRQKREEVKIGLRTPLRRLTIVNRDRALLEELARLESYVKTELNVLAVSYD
                     DDEGAYIEVVAKPNFPVLGKRLGKRMKAFQSAIATLSPEQIAAFQAEGEIELDGEVFN
                     LEEIEVLQKARPGTNTISNSRIAVDLDCVLDDELVRGGYAREIVNRIQRRRKELELNV
                     ADRIEVCFAGDRALVEAAAEHRDYIAAETLATDFAEGDPGSQAADSEIDGKAFRFSVV
                     RAGS"
BASE COUNT          602 a         1188 c         1110 g          621 t
ORIGIN      
        1 cttgctggta tccagaccgc tcgcccagag aaagcgccgg tcatcggtca ggcccgagtg
       61 atgcgcctcg ttgcggccgc ccaccgacag gctgtgcacc acctctccat aggttttcga
      121 ggtcgggttg acgtccacgg tcacgagctt gtcctgaccg tcgcccacgc cctccatccc
      181 gagcgtccag acgtaaacga aatcttcctg cccgacgatc ttggccatgt agggtgacat
      241 gcacgtctcg tctgcccaga cgggcatggc gagcaacgcg cccgccgcca gctgcgcgag
      301 catcttcctg atagtcttca tcggttcccc ctgttctgga acggtctcgc aggtatccta
      361 tcaccccaat ggagtcggtc ctaagacaca tgtggcgagg cccgatcacc agtcaggcag
      421 tagccgccct catgctgacc gcgtgctgca cctgggccgt cacgcccggt gatccgagag
      481 acgggtacga gcacgccgac tacgagacgg catcgaggca actcagcgca ctccgcggat
      541 cggcgctggc gctcgcggag ctggcaggca acccgcctct cggcttgccg cccctgccgg
      601 cagagcggtt gccggtggcg gcggccatcg acctcggccg acggctgttc ttcgacaggc
      661 gactgtctgc caacgcgacg ctgtcctgcg ccatgtgtca cgtgccggag cagggtttca
      721 cccagaacga gctgaaaacc ccggtgggca tcgaaggccg cttcgtgcac cgcaacgcac
      781 cctcgctcta caacgtggct taccaggaag ccctgtttca cgacggccgg gaagacgacc
      841 tggccaacca gatctgggcc ccgctgctgg cagccaacga gatgggcaac gcctcccgcg
      901 aggccgtggt cgagcgcctg gggggcctgg aggagtacga gggcgagttt ctccgggtgt
      961 tcggcgacgg gatcagcgcc gcaacactgg gtcgggcgct ggccagctac cagacggctc
     1021 ttctgtcggc cgactccccc ttcgaccgct ggtacttcgg cggcgaggcc gatgccgtcg
     1081 gcgcggaggt caaacgcgga tttcgatcat tcgtcaatga aggttgcgcg acctgccatc
     1141 agctgcagac gggccacgcc acgttcacgg acggggccta ccacgatacc ggcatcggct
     1201 tcctggcagc ggccagtgcg ggcacgccgg cccgccgcct gcagatcgcg cccggggtca
     1261 tcatcgacgt cgaaacggac atcgcgaccc cgcacgtcga ggacgacggc cgagcagccg
     1321 tgacggggga tccggccgac cgctggcgat accgaacgcc gggtttgcgc aacgtggcgc
     1381 tgagcgcgcc ttacatgcac gacggctcgc tcgccaccct ggaggcggtc atcgactact
     1441 acgcagcagg gggcgctccc cacgacggcc aggacgcccg aatcaggccg ctgtctctgg
     1501 gcgctgatga acgcgccgac ctcgtcgcct tcctgcaggc gctgacaggc agcaacgtcg
     1561 acgccctggc tggcgacgcc agggccgcgc ccatcggcga cacgcatcac ggtccttgag
     1621 cgcggtctga gtgctcagct cccggcgcgg acgacggaga agcggaacgc cttgccatcg
     1681 atctcggagt ccgccgcctg ggatcccgga tcgccctcgg caaagtcggt ggccagagtc
     1741 tccgcagcga tgtagtcacg gtgttcggcg gccgcctcga ccagagcccg atccccagcg
     1801 aagcagacct cgatgcgatc cgccacgttc agctccagct ccttgcgccg gcgctgaatg
     1861 cggttgacga tctcgcgggc gtaaccgccg cgcacgagct cgtcgtccag aacgcagtcc
     1921 agatcaacgg cgatacggct gtttgagatg gtgttggtcc caggtcgcgc tttctgcagc
     1981 acctcgatct cctcgaggtt gaagacctcc ccgtcgagct cgatctcgcc ctcggcctga
     2041 aaggcggcga tctgctcggg agacaaggtc gcaatcgcgc tctgaaaggc cttcatccgc
     2101 ttgcccagcc gcttgccgag cacgggaaag ttcggcttgg cgaccacctc gatgtacgcg
     2161 ccttcgtcgt catcgtagct caccgccagg acgttgagct ccgttttgac atagctctcc
     2221 agccgggcga gctcctcgag cagcgctcgg tcgcggttga cgatggtcag gcgtctgagc
     2281 ggcgtgcgaa gaccgatctt cacctcctcg cgcttctgac gaccgagcag cacgacctgc
     2341 tgcatgcggt cgacggcttc ttccagctcg ctcctgatca gcgcgccctc cggctccgga
     2401 taatcgcaca gatgcaccga ctcgggcttg gcccggccgc cgcccagcgc cgacagctcc
     2461 tggtacacgt gctccgcaag gaacggcgcg aaaggcgcca tcacctggca gagctcgtac
     2521 agcgcggcgt agagcgtgct gtaggcggca atcttgtctg ccgtgatgtc ctcgccccag
     2581 aaccgctgac ggttgaggcg gatgtaccag ttggtcaggt cctcgatgaa atcgaacagc
     2641 tgcgggacca cgttgtagag cttatagacc tccatctccc ggccgacgtt tgccttgagg
     2701 gtctgcaggc gcgagaggat ccagtgatcc aggacgttgt cgccggtgtg ctgacccttg
     2761 tccggcgacc actgatcgat ctccgcgtag gtcttcagga aagtgaacgc gttgtaccag
     2821 ggcagcaggg cccggcgcgt catgtcccgc acgccggcat cggcgaaacg ctgctcctcc
     2881 ccccgcatca gccccgagtt gatgagatac aggcgcaggg cgtccgcgcc gtaagtttcc
     2941 atgagctcat cgggcggggt gtagttgcga agccgcttgg acatcttctt gccgtcctcc
     3001 gccatcacca tcccgttgac gatgacgttg cggaaagcgg gcttgtcgta gagcgcggcg
     3061 gctagaaccg tcagcgtgta gaaccagcct cgcgtctggt ccagcccttc ggcgatgaac
     3121 tcggccggaa agccggcctt gaagaccttc tcgttctcga acggatagtg cagctgggca
     3181 tagggcatgg agccagactc gaaccagcag tccagcacct cctcaatccg atggtaggtg
     3241 ccttcctcgc cttggagcgt aaaggtcagc ggatccacgt gctcgcggtg cagatcatcg
     3301 acgcggacgc cggtgagcgc ttccagctcg tcgatggagc cgatgcagac ctcattgccc
     3361 gtgacgtcgt tgatccagat gggaatcggc gtgccccaga cccggttgcg ggagatggac
     3421 cagtcgatgg cgccttccag ccagtttccg aaccgcccgg tcttgatgtg gtcgggaacc
     3481 cagcggatct gctggttgtt tttgacaagc ttgtcaacga a
//
LOCUS       DBNHLM010000350        17118 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1549573, whole genome shotgun sequence.
ACCESSION   DBNHLM010000350 DBNHLM010000000
VERSION     DBNHLM010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 17118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17118
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1549573"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..331)
                     /locus_tag="AC16QF_16065"
     CDS             complement(146..331)
                     /locus_tag="AC16QF_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450126.1"
                     /translation="MTRGLITFLAMAAPGLAFAGPECTVFICPDPVPELDGGMAVLAL
                     GLTAAVVAIVRERLRRR"
     gene            520..1971
                     /locus_tag="AC16QF_16070"
     CDS             520..1971
                     /locus_tag="AC16QF_16070"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIR6450127.1"
                     /translation="MRLDDKGLLRTAAYVAGEWVAADSGETFDVRNPATGEVVAAVAR
                     CGAAETRRAIEAAERAQVDWRARTAKERARVLRRLYELMMLHQEDLAQILTAEMGKPI
                     VEARGEIAYGASYLEWFAEEAKRVYGDTIPGPAPDKRIVTIKQPVGVVGCITPWNFPN
                     AMLTRKVGPALAAGCAVVCKPATATPLSALAIAELGARAGVPAGVLNVLAGSTREIGS
                     EITGNPAVRKLTFTGSTGVGKALIEQCAATVKRTSMELGGNAPFIVFDDADVDAAVAG
                     AIASKYRNAGQTCVCANRLLVQAGVYEEFAEKLSAAVAEFRLGDGAEETTTIGPLIDA
                     GAADEVLAMIDDAIANGASAVIGGARAAQGGSFVQPTVLTEVTPRMRVFREEIFGPVA
                     PLIRFETEDEAIRLANDTEFGLASYFYARDVGRIWRVAEGLEYGIVGINEGIVSNEMA
                     PFGGVKESGNGREGSKYGMDDYLEIKYLCMGGI"
     gene            1986..4127
                     /locus_tag="AC16QF_16075"
     CDS             1986..4127
                     /locus_tag="AC16QF_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450128.1"
                     /translation="MWFHEKAKHRPVSLGPYPLETLPRDPAQSEIELRMPLARQLPLE
                     ESASELGAATGRYLSLFLEFRDGEVAPQPAPVPDDVDLRADDVKGLAYFFDADHVGIC
                     ELPSGARLIESDALDHAMAVVILVRFGRQPEADNLARDWIAGAEHDVAELRAAEIAVT
                     VAGYIRQLGYPATAHVRRATTVDLDRLAVLSGVCVRRDGAVCSPYFGDGFAAAAVTTD
                     YALAVDAPLAPDQGKARGRRYHRGQNGAVSGRERRRRDRRASHLSRYPMEQVRRVERP
                     TTLIIDDEVPRVPKRANFFTRAAFGDLGEKSQREVRRFAYKHPSTAGTMQLIRSMVSL
                     QDGEIAGSRWASLDSKANAAAIKSLGYHLGADLVGICEIPPYAWYSHQADGTLIEPYH
                     RFAVVMLIDQGFDTMEGASGDDWISGAQSMRGYVRGAEIAGVMAEFLRSVGVSARPQT
                     NADSDVLHIPLILWAGLGELSRIGELVLNPFVGPRFKSVVLTTDLPLEVDAPIDFGLQ
                     YFCNHCLKCARECPCNAIPTGSKVMFNGYEIWKPDVERCTRYRITNQKGAACGRCMKT
                     CPLNKVVGADGALAHRAGNWLAIHLPWLRPLLVPIATRLDDRTGYGARNPVKKWWFDL
                     EIVDGVCVHAEANQRDLDVGRSAASRRDTVGYYPADTMPAPDAQGPVPVNHREAVGRS
                     ALLETPADARRRKRKGEPGPSHYVPLVDVTD"
     gene            4228..4683
                     /locus_tag="AC16QF_16080"
     CDS             4228..4683
                     /locus_tag="AC16QF_16080"
                     /inference="COORDINATES: protein motif:HMM:NF047172.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6450129.1"
                     /translation="MNDFDRSLPMLLYRALDLIMPRFRAIFGGFGLTEQQWRVLRVLW
                     QRDGRALLELADATLISAPSMVGVVDRLARSGLVERRRSQSDRRVVRVFLTGRGRDLE
                     EQVTPLVDAAYADLRGSVGEGDWEVLLGALARLNAGLADLDRIDEVANR"
     gene            4708..6159
                     /gene="hpaB"
                     /locus_tag="AC16QF_16085"
     CDS             4708..6159
                     /gene="hpaB"
                     /locus_tag="AC16QF_16085"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HIR6450130.1"
                     /translation="MGIRTGAQYLEGLRDGREVWMHGRRIEDVTTEPGLCRGAHTLAD
                     FLDRVHEPAHREKLSYAEAGSDERFSLSFLKPGSKEDVERRGAAFYEWAKWSGGMFGR
                     TPDYKNASVMAFAAASEFLAGEGGRPEFAENMRRYYEKARVEDLVLTHTLVNPQFNAA
                     LAAAGRSSPEVALQIVGENDAGVVVNGARLLATLGPHADEIEVFPSTLLKASPENVPF
                     AIAFTIPIETRGLRLICRDTYDAGKSHFDAPLASRFEEMDAVVIFDNVLVPWERVFMY
                     QAPLLCNRAFAETNAVVHMMHQVACGKLAKAEFIVGLLCAMAKASGKDKDANVKGQIA
                     EAMWIVETVRAFLFSAEQQAEPDRWGLWVPKRLPLDTSRNLFPRLYPKLVETVHMLGS
                     SSLMATPCEADFENAIAGDVEQYFQLTNLESRDRVALFRLAHDVAVSGFGNRQVLYER
                     FFFGPQPVMASIYYDLYDKDSVMDRVTEMLNGC"
     gene            6282..6713
                     /gene="hpaR"
                     /locus_tag="AC16QF_16090"
     CDS             6282..6713
                     /gene="hpaR"
                     /locus_tag="AC16QF_16090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HIR6450131.1"
                     /translation="MLLLRARETSMAYFRPSLQERGLTEQQWRVLRALQESGELSASG
                     LSHACVILAPSMTRILRRLAEEGLVSSKRSRRDGRELTVRLTRAGERLIDEIAPISER
                     QYALIRERLQPEKLEQLHSLLHDFVELDGEHLKGAASGETQ"
     gene            6710..8233
                     /gene="hpaE"
                     /locus_tag="AC16QF_16095"
     CDS             6710..8233
                     /gene="hpaE"
                     /locus_tag="AC16QF_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010324201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HIR6450132.1"
                     /translation="MSQLLHENLAKLDRHLARFRNRTLGHLIDGEEAEGVSGETFTNT
                     TPVDDSPVNEVARGAEEDVTRATRAAVRAFGEWRDMPGARRRGLLHGIADAIEARAEE
                     IALVESYDTGQPLRFMSSAALRGAENFRFYADRAPEARNGLSTPADQHVNYSVRQPIG
                     PVGVITPWNTPFMLSTWKIAPALAAGCTVVHKPAEWSPLSASILAEICRDAGLPAGVL
                     NTVHGLGEEAGVALTEQPDIRAIAFVGESQTGRAIMSQGASTLKRVHFELGGKNPVIV
                     FDDADLERALDACTFMIYSLNGERCTSSSRLLVQATIYDEFCARVAERAGQIRVGHPL
                     DPATEVGPLIHPRHLEKVLGYCALAQTEGATVAVGGGPPGDAAAGNYVAPTLFTHARN
                     DMRIAQEEIFGPVLTAIPFDDEAEAVELANQVEFGLAGYVWTGDVGRSHRLARDLDAG
                     MIWVNSENNRHLPTPFGGMKASGIGRDGGDYSFDFYMETKNVCISLGKHRVPSLGKG"
     gene            8241..8555
                     /locus_tag="AC16QF_16100"
     CDS             8241..8555
                     /locus_tag="AC16QF_16100"
                     /inference="COORDINATES: protein motif:HMM:NF019366.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450133.1"
                     /translation="MSLYYVQKLLYQLNRDPHARRRYEEDFDELLAEYDLDAEELHAI
                     REPDIGLLYVMGVNGQILMHYAALRGYAWDEYIKAMRDGLAEHGDVRAGLYVTTDGRG
                     AV"
     gene            8555..9373
                     /locus_tag="AC16QF_16105"
     CDS             8555..9373
                     /locus_tag="AC16QF_16105"
                     /inference="COORDINATES: protein motif:HMM:NF014899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DODA-type extradiol aromatic ring-opening family
                     dioxygenase"
                     /protein_id="HIR6450134.1"
                     /translation="MGVVFAGVVSHAPGITGRAERADPALRDALYENFYRLGRELKES
                     RPDALIVIAAEHFANFFMDNMPAYCMGMADYYEGPIEDEQWLGISRVRIPGNAALAGS
                     LIESMLAEFDLAYAQEWKFDHGVMVPLHFLTPEYDIPVIPVNVNCQGPPLTPLARAYD
                     FGRALRRACDARPERIAIIGTGGISHWPATPDSGKINEAWDRAFLESWLANDRAALTS
                     FTDEETYLEAGQGGFEIRTFIAVSGATQGCAAELYFYEPIPIFAVGCTVARVHV"
     gene            9501..9980
                     /locus_tag="AC16QF_16110"
     CDS             9501..9980
                     /locus_tag="AC16QF_16110"
                     /inference="COORDINATES: protein motif:HMM:NF024375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6450135.1"
                     /translation="MALQNIKPINDQLADIIVRADEPWIEQEPGMAWIKVLWTGAETG
                     RWVALFRWNKGYTAAPHKHLSDAHTYVLKGKLQVRDGVLNAGDYDYEPNGVLHGATTA
                     LEDTEYLFVCEGPIVFFDENGLTNYLSWEELARMRDAAKAGGDASMGISVTAESSAG"
     gene            complement(10030..10440)
                     /locus_tag="AC16QF_16115"
     CDS             complement(10030..10440)
                     /locus_tag="AC16QF_16115"
                     /inference="COORDINATES: protein motif:HMM:NF016701.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6450136.1"
                     /translation="MTVEGGCYCGAVRYKAEGDPVFSAQCHCRECQYLTGGAPNMFMA
                     VPRSGFEYTKGEPSAFTRTDIENPVTREFCGNCGTHLLTRANAMPDAVILKVGSMDDP
                     GQYPGPQVAIFCMDKQTFHTVPAGVPEFERLPGG"
     gene            complement(10564..11424)
                     /locus_tag="AC16QF_16120"
     CDS             complement(10564..11424)
                     /locus_tag="AC16QF_16120"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR6450137.1"
                     /translation="MRFLTYRLEGRTAFGAVIDGGIVNLAERLPAFGSLRDLLAAGAL
                     EQARRVAEDADPDHRLQDVGFLPPIPDPQKICCIGVNYGNRNEEYADAAPAARYPSLF
                     LRTRESLVGHLEPIVRPPESEQLDYEGEIVIVIGKAGRRIPESQARAHVAGLTIMNEG
                     TIRDWLRHAKFNVTQGKNFERSGSVGPWLVSLDEIERFDDLHLTTKVNGELRQDDRTD
                     NLLFSFEYLISYLSTFMRLQPGDLISTGTPTGAGARFDPPRYLKPGDVVEVAVDGIGT
                     LRNTVEDEVA"
     gene            11534..11950
                     /locus_tag="AC16QF_16125"
     CDS             11534..11950
                     /locus_tag="AC16QF_16125"
                     /inference="COORDINATES: protein motif:HMM:NF014958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate isomerase"
                     /protein_id="HIR6450138.1"
                     /translation="MPHLIIEYSANIDEELMLDALMLRLRDSAVATGVFPLPGIRVRA
                     ARRDRFVVADGDPENAFVHLMVRIGHGRTPEVRRQVADRLFAVLCDHLREAATRRGLG
                     LSLEIQEIDPVGSLKRNNLHERLAGRAAGHVSVKVE"
     gene            11947..12582
                     /locus_tag="AC16QF_16130"
     CDS             11947..12582
                     /locus_tag="AC16QF_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450139.1"
                     /translation="MKIGPVLICAFALAMAPFAGAQSVSAWDLVYPSNFNWEIRTHRV
                     RVANDLASRVSLLVAVVPRQTEEESARLRETEQTLEARGADASPRDRSRLYLSRAYQH
                     RRLLELLADTLDALQCVRSAEELPREVHCWTRAAVNLMDEETVDVALTVLRDARMVPR
                     DEDMPVKAQDPKVWYGEYGKGIVRHIVMPYLAGLAAPRTPAATEEEVSHDG"
     gene            12572..13192
                     /locus_tag="AC16QF_16135"
     CDS             12572..13192
                     /locus_tag="AC16QF_16135"
                     /inference="COORDINATES: protein motif:HMM:NF019339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6450140.1"
                     /translation="MMVERAPPIMLTLLLSSTLAAASETVLFNESAAFECYQAALHDG
                     GEYDIESCSRALEYQALTTIDRAATHSNRGILYARIGDIRNALRDHNRAVKLAPEVGS
                     IYINRSNTLVRAKRYEGAMRDLEKAIEIADDSLAYAHYNRALLFQKLGDLQAARADAE
                     QAAEIAPDSPAYRRFLAAFGETEGDPLLPDAEPGNPPEEPGGEGQD"
     gene            complement(13183..16275)
                     /locus_tag="AC16QF_16140"
     CDS             complement(13183..16275)
                     /locus_tag="AC16QF_16140"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6450141.1"
                     /translation="MWLSDISVQRPVFASVISLALIAFGALSFNLLPLREYPDINPPL
                     VSISTAYVGASAEVIESRVTQVIEDQISGIEGIKSISSSSRDEASNINIEFELNRNID
                     EAANDVRDRVARIVDRLPDDVELPIVAKQDSDARPVMYISLTSSRMSVMELNDYAERY
                     IVDRFEIIPGVASAAMHGSGKPSMRIWLDRLALAARDLTVIDIEDALRRENLELPAGR
                     IESAEREFRVRLARGYQSDEDFRQLVLVVGGDGHLIRLGEVADVNLAPSNTRESFRAN
                     RQNTVGLAITKQSTANTLATLEAVKAEIARVNEALPDHMEFVPSSDDSLFIREAINAV
                     YQTIAITTVLVSLVIFVFLGSIRSMIIPAVTIPVCLTSAFIALALFGYSVNLVTILAL
                     VLCIGLVVDDAIVMLENIHRRVERGEPPLVAAYRGSRQVAFAVIATTVVLVAVFTPIM
                     FLRDNMGVIFSELAVTVSAAVVFSSVLALSLTPMMSSKLLRKTTAKNPVTRMVERAFA
                     RLEAGYQRLLKRLLPYSWAAAVITLLVIGLAYWLMGMVPREYAPREDQGFFMAQVLAP
                     EGTGFDRMQEHMGGVEAPLMQMQDEGLISRVLVRVPSWGGTSPNTGMVIVSLEPWRER
                     DATTREIIDRASTAWEEIPAVRIFAFERSGLSRGGGGRPVQFVIGGPNYETLAQWRDL
                     LIARAMEYPGMTRVDSDLKETQPQLIVRVDKNRAAALGISVQNVGRTLAAMMSERRVT
                     TFVQDGEEYDVILQAKEDQRATAADLQNIYVRSESTGRLVPLANLIEVEETAGAGRLN
                     RYNRLRAVTIEAGLAPGYALADALEFLETVVREELPESAQVDYRGESLEFKEASGSLL
                     FTFAIALLIVYLVLAAQFESFVHPLVILLTVPMAIAGALLGLKLTGGTLNIYSQIGIV
                     MLIGIAAKNGVLIVEFINQLRDAGRSFDDAIVEAAGIRLRPVIMTTISTVMGSTPLIL
                     ATGAGSTSRSMLGVVIFSGVSLATLLTLFIVPAFYHLLARRTGSPEETARRLKGLIQS
                     "
     gene            complement(16281..>17118)
                     /locus_tag="AC16QF_16145"
     CDS             complement(16281..>17118)
                     /locus_tag="AC16QF_16145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6450142.1"
                     /translation="TDSVSQVRFEDGDYVNAGDVLVELTNEEETALLAEAEANARDTR
                     TQLDRLEDLVLQGSVPISQVDEARARHSAAVARYQSVVARLEDRLITAPFSGVLGFRQ
                     VSTGTLITPGTPITTLDDVSVIKLDFSIPEIHLSRLQPGQRIEAESPAYSGSVFDATV
                     STIGSRVDPVTRAAVVRAHIDNGDMRLRPGMLLTARLTTSEREVLMIPEAALLQRGTQ
                     VRVFTEVDGTARSRLITHGVRDAGWVEVREGLTEGERVITDGLIKVRDGSPITTVRAE
                     QG"
BASE COUNT         3014 a         5338 c         5742 g         3024 t
ORIGIN      
        1 aatgtagcgt gtggtgccgt tgtggcccga atgtagggaa gcagcctacg gtgttcccgg
       61 ttcgcttaca ttcggggccc gcgctgaggc cgctcgagcg aagctgatgt aagccgcctg
      121 ccctcgcgcg gccaagcggc ctatgtcagc ggcggcgcag acgctcccgc acgatggcga
      181 cgacggcggc ggtcagaccc agtgccagta ccgccattcc cccgtcgagt tcgggtaccg
      241 gatcgggaca aataaaaaca gtacattctg gtccagcaaa agccagcccc ggcgccgcca
      301 tcgccaaaaa tgtaatcaat ccacgtgtca tttcgagcgc tccccctctg ggtgtttaat
      361 agaaatagtc agtattcttg atattaaata ggcgcagcgt gaatatcaag taaattgttt
      421 aaaaattccc tcgcttgggt ggtctgtctc acaccggcgc caggcggcca ggtcggcgtt
      481 agaatgggcg ggtatttcac cggcgggcag gggataggcg tgcgtctaga cgacaagggt
      541 ctgctgcgaa ccgcagctta cgtggccggc gagtgggtgg cggcagacag cggcgaaacc
      601 ttcgacgttc gcaatccggc aaccggagaa gtggttgccg cggtggcgcg gtgcggcgcc
      661 gcggagaccc gccgtgccat cgaggcagcg gagcgtgctc aggtggactg gcgggcgaga
      721 acggccaagg aacgagcgcg cgtgctgcgc cggctctacg agttgatgat gctgcatcag
      781 gaggatcttg cgcagattct taccgccgag atgggcaagc ccatcgttga agcgcgcggc
      841 gagatcgcct acggggcaag ctatctggaa tggttcgccg aagaggccaa gcgcgtttac
      901 ggcgacacca tcccgggccc cgcgccggac aagcggatcg tcaccatcaa gcagcccgtg
      961 ggcgtcgtcg gctgcatcac cccgtggaat ttccccaatg ccatgctgac ccgcaaggtc
     1021 gggcccgccc tcgccgccgg ctgcgcggtg gtgtgcaagc cggccaccgc gacgccgctg
     1081 tctgcactgg ccatcgcgga gcttggggcg cgggccggcg tgccggcggg tgttctgaac
     1141 gtgctggccg gaagcacccg ggaaattggc agcgagatca ccggcaaccc ggcggtgcgc
     1201 aagctgacct tcaccggatc caccggcgtg ggcaaagcgc tcatcgagca gtgcgcggcc
     1261 acggtcaagc gcacgtccat ggagctcggg ggcaacgcgc ccttcatcgt cttcgacgac
     1321 gcggacgtgg acgccgccgt cgcgggggcc atcgcatcca agtaccgaaa cgcgggccag
     1381 acgtgcgtgt gcgccaaccg cctgctcgtc caggcagggg tatacgagga atttgcggag
     1441 aagctctcgg cggcggtggc ggagttccgg ctgggcgacg gggcagagga gaccaccacc
     1501 atcggtcccc tcatcgatgc aggggctgcc gacgaggtgc tggcaatgat cgacgacgcg
     1561 atcgccaacg gcgccagcgc ggtgatcggc ggggcgcggg ccgcccaggg gggctccttc
     1621 gtccagccaa ccgttctcac ggaagtgacc ccgcgcatgc gggtgtttcg cgaagagatc
     1681 ttcgggccgg tggcgccgct catccggttc gagacggagg acgaagcgat ccggctggcc
     1741 aacgataccg agttcgggct ggcgtcgtat ttctacgccc gggacgtggg tcgaatctgg
     1801 cgcgtcgccg agggactcga atacggtatc gtcgggatca acgagggcat cgtctcgaac
     1861 gagatggcgc ctttcggcgg ggtcaaggag tcgggtaacg gccgcgaggg atcgaagtac
     1921 gggatggacg actatctgga gatcaaatat ctctgcatgg gcgggatcta gcggggctcg
     1981 ccgtcatgtg gtttcacgag aaggcaaagc atcggcccgt gagcctcggc ccctatcccc
     2041 tggagacgtt gccccgggat ccggctcaga gcgagatcga gctgcgcatg ccgctggcaa
     2101 ggcagctgcc gctggaagag tcggcgtctg agcttggcgc ggccaccggg cgctacctct
     2161 cgctcttcct ggagtttcgc gacggcgagg tggctcctca gcccgcgccc gtccccgacg
     2221 acgtcgacct ccgtgcggac gacgtcaaag gtctggccta cttcttcgat gcggatcacg
     2281 tgggtatctg cgagctccca tcgggcgcac gactgattga atctgatgcc ctggatcacg
     2341 ccatggccgt cgtcatcctc gttcgcttcg ggcgccagcc ggaagccgac aacctcgccc
     2401 gggactggat tgcgggcgcc gagcacgatg tggcggagct cagagcggcg gagatcgccg
     2461 tcacggtggc cggctacatc cggcagctgg gctacccggc caccgctcac gtccggcggg
     2521 ccaccaccgt ggacctggac cggctggccg tcctgtccgg cgtctgcgtg cgcagggacg
     2581 gtgccgtctg ctccccgtac tttggtgacg gcttcgctgc agccgcggtg accacggatt
     2641 acgccctggc ggtggatgcc cctctggccc cggaccaggg caaggcccgg ggccggcgct
     2701 accaccgggg tcaaaacggc gcggtctccg gcagagaacg ccgtcgccgg gaccggcgtg
     2761 cgtctcactt gagccgctat cccatggagc aggtccgtcg ggtcgaacgg ccgaccacgc
     2821 tgatcatcga cgatgaggtt cccagggttc cgaagcgggc gaactttttt acgcgggcgg
     2881 cttttggcga ccttggcgag aagtcccagc gcgaggtccg ccggtttgcc tacaagcacc
     2941 cttccacggc ggggaccatg cagctcatca ggagcatggt gtcgttgcag gacggggaga
     3001 tcgccggttc ccgttgggcc tcgctggact cgaaagccaa cgctgcggcc ataaagtcgc
     3061 tcggctacca cctcggggcg gatctcgtgg gcatctgcga gattccgccg tatgcctggt
     3121 actcccatca ggcagacgga acgctcatcg agccgtatca ccgctttgct gtggtgatgc
     3181 tcatcgatca gggcttcgac accatggagg gcgccagcgg ggacgactgg atttccggtg
     3241 cgcagtccat gcgaggctac gtgcgtggtg cggaaatcgc tggcgtgatg gccgagtttc
     3301 tgcgctccgt gggagtctcg gcgcgaccgc agaccaacgc ggacagcgac gttctgcaca
     3361 taccgctgat tctgtgggcg ggccttggcg agctcagccg aatcggcgag ctggtgctga
     3421 acccgttcgt cggtccccgt ttcaagtccg tggtgctgac cacggatctg cccctggagg
     3481 tggacgcgcc catagacttc gggctgcagt atttctgcaa tcattgcctg aagtgcgcgc
     3541 gggaatgtcc ctgcaacgcg attcctacgg gctccaaggt catgttcaac gggtacgaga
     3601 tctggaagcc ggacgtggag cggtgcacgc gctaccgcat taccaaccag aaaggggcgg
     3661 cctgcggaag gtgcatgaag acctgcccgc tcaacaaggt ggtgggcgcc gatggcgctc
     3721 ttgcgcaccg agccggcaac tggctggcaa ttcatcttcc ctggctgcgg ccgctcctgg
     3781 ttcccatcgc cacgcggctg gatgaccgga ccggctacgg ggcccgtaac ccggtgaaga
     3841 agtggtggtt cgatctggag atcgtcgacg gcgtatgcgt ccatgcagaa gcgaaccagc
     3901 gcgacctcga cgtcggtcgg tcggcggctt cccggcgcga taccgtgggc tactatccgg
     3961 cggatacgat gccggctccc gatgctcagg gtcccgttcc cgtcaaccac cgggaagccg
     4021 ttggccggag cgccttgctg gaaacccctg ccgatgcgcg tcgccggaaa cgcaaaggcg
     4081 agcctggccc ctctcattac gttccgctcg tcgacgtcac cgattgaccc caccgctttt
     4141 cggccccctt gcagccgatg attacccggt tgacgcgtct cgagatttaa ctatcatttt
     4201 aattattaat acatgaacta aaagcgcgtg aacgacttcg accgatccct gcccatgctg
     4261 ctttaccggg cccttgacct gatcatgccc agatttcgcg ccatattcgg cgggttcggg
     4321 ctcactgagc agcagtggcg cgtgctgcgg gtgctgtggc agcgggatgg tcgtgcgctg
     4381 ctcgaacttg ccgacgccac cctgatctcg gcgcccagca tggtcggggt ggtggatcgc
     4441 ctggcgcgca gcggtctggt ggaacgacgc cggtcgcaga gtgatcggcg ggtcgtgcgg
     4501 gtgtttctca ccgggcgcgg cagggactta gaggaacagg tcacgcccct ggtggacgcg
     4561 gcctacgcgg acctgcgcgg gagcgtgggg gagggtgact gggaggttct gctcggcgcg
     4621 ctggcccggc tgaatgcagg gcttgctgac ctggaccgga tcgacgaggt ggccaatcgc
     4681 tgaaccggcg aagcgaggag atttacgatg ggaatcagaa cgggtgcgca atacctcgaa
     4741 gggctcagag acggccgtga ggtctggatg catggcaggc ggatagagga cgtgacgacc
     4801 gagccggggc tctgccgcgg tgcccacacg ctggcggatt ttctggatcg ggttcacgag
     4861 cctgcccatc gcgaaaaact ctcctatgcc gaggcgggta gcgacgagcg attcagcctg
     4921 tccttcctca agcccgggtc caaggaggac gtggagcgcc gaggtgccgc gttctacgaa
     4981 tgggcgaagt ggtcgggcgg catgttcgga cgcacgcccg actacaagaa cgcctcggtg
     5041 atggcgtttg ccgcggcgtc ggaatttctc gccggggagg gcgggcgacc ggagttcgcg
     5101 gagaacatgc gtcgctacta cgaaaaggcc cgtgtggagg acctggtgct cacccacacg
     5161 ctggtcaatc cgcagttcaa cgcggcgctt gcggcggcgg gtcgatcgag tcccgaggtg
     5221 gccctgcaga tcgtgggcga gaacgatgcc ggcgtggtcg tcaacggcgc gcggctgctt
     5281 gccacgctgg gcccccatgc ggacgagatc gaggtgtttc cgtccaccct cctgaaggcc
     5341 tcgccggaga acgtgccctt tgccatcgct ttcaccatcc ccatcgaaac caggggcctg
     5401 cggctcatct gtcgggacac ctacgacgcg ggcaagagcc atttcgatgc gcctctggca
     5461 tcccgattcg aggaaatgga cgcggtggtc atcttcgaca acgtgttggt gccctgggag
     5521 cgtgtgttca tgtaccaggc gccgctgctg tgcaaccgcg cgtttgcgga gaccaacgcc
     5581 gtcgtgcaca tgatgcatca ggtggcctgc gggaagctgg ccaaggcgga gttcatcgtg
     5641 ggtctgctct gcgccatggc caaggcctcg ggcaaagaca aggatgccaa cgtcaaaggc
     5701 cagatcgcgg aagcgatgtg gatagtcgaa accgtccggg cctttctgtt cagcgccgag
     5761 cagcaggcag aacctgatcg ctggggcctg tgggtaccca agcgcctgcc tctggatacg
     5821 tcccgtaacc tgttcccgag gctttatccg aagctcgtgg aaaccgtgca catgctcggc
     5881 tctagttcgc tcatggccac gccctgcgag gccgatttcg aaaacgccat tgccggggac
     5941 gtggagcaat actttcagct caccaatctg gagagcaggg accgcgttgc cctgttccgg
     6001 ctggcgcacg acgtcgcggt ctcaggattc ggcaaccgtc aggtgctcta cgagcgcttt
     6061 ttcttcggtc cccagccggt catggcctcg atctactacg atctttacga taaggacagc
     6121 gtgatggatc gcgtcacgga aatgctcaat ggctgttgag acgacgggtt ccagtggcat
     6181 tgcggatgca acacagggaa ttaacatgtt aaataatcgc tcatcttgga aggccggagc
     6241 cttgcgtggc gcaaccccgc aaattcagtg agtctctgcc catgcttctg ctccgggccc
     6301 gcgagacctc tatggcctat ttccggcctt ccctgcagga gcgggggctc accgagcagc
     6361 aatggcgtgt gcttcgggcg ttgcaggaat ctggcgagct gtcggcgtcg gggctttcac
     6421 acgcgtgcgt gattctggcg cccagcatga cgcggatact gcgtcggctt gccgaggagg
     6481 ggctcgtttc gtcgaagcgg tcgcgccgcg acggcaggga gctcacggtg aggttgaccc
     6541 gcgcgggcga gcgtctgatc gacgagatcg cgcccatcag cgagcgtcag tacgcgctca
     6601 tccgcgaacg gctgcaaccg gaaaagctgg agcagctcca cagcctgctg cacgacttcg
     6661 tggaactgga tggcgagcac ctgaaagggg ccgcatcggg agagacccaa tgagccaact
     6721 gcttcacgaa aacctggcaa agctcgaccg gcacctggcg cgtttccgca accgaacgct
     6781 ggggcacctc atcgacgggg aggaagccga aggcgtctcg ggcgagacgt ttacgaacac
     6841 taccccggtg gacgactctc cggtgaacga ggtggcccgg ggtgccgagg aagacgtaac
     6901 cagagcgacg cgggcagccg taagggcgtt tggcgagtgg cgtgacatgc cgggtgcccg
     6961 ccggcgcggc ctgctccacg ggatcgccga tgccatcgag gcgcgggctg aggagattgc
     7021 cctggtggag tcgtacgaca ccggccagcc gctgcgtttc atgagcagcg cggcgctgcg
     7081 cggagccgaa aacttcaggt tttatgccga ccgagcgccg gaagcccgca acggcctctc
     7141 cactcccgcc gatcagcatg tgaactacag tgtccgccaa ccgatcgggc cggtcggcgt
     7201 catcacccct tggaacacgc cgttcatgct ctccacctgg aagatcgctc cggctctggc
     7261 cgccggctgc acggtggtgc acaagccggc agagtggagc cccctctcgg ccagcatact
     7321 cgcggagatc tgtcgagacg ccggtctgcc tgccggcgtg ctcaacacgg ttcatgggct
     7381 aggcgaggag gctggcgtgg cgctcacgga gcagccggac atccgagcca tcgctttcgt
     7441 gggcgagtcc caaacgggcc gcgcgatcat gagccagggc gcgagcacgc tcaagcgcgt
     7501 gcacttcgag ctcggcggga agaatccggt catcgtgttc gacgacgccg atctcgaacg
     7561 cgcgctggat gcctgcacgt tcatgatcta cagcctcaac ggcgagcggt gcacctcgtc
     7621 gagccgactg ctcgtgcagg cgacgatcta cgatgagttc tgcgcccggg tggctgagcg
     7681 agccgggcag atccgtgtgg gccacccgct ggatcccgcc accgaggtgg gaccgctgat
     7741 tcatccgcgc cacctcgaaa aggtgctcgg gtactgcgcg ctggcgcaga ccgaaggggc
     7801 taccgtggcg gtcgggggcg gccctccggg ggacgcggcc gccgggaact acgtggcgcc
     7861 cacgctcttt acccacgcgc gcaacgacat gcgtatcgcc caggaggaga tattcggtcc
     7921 cgtgctgacg gcgatccctt tcgacgacga agccgaggcc gttgagcttg ccaaccaggt
     7981 ggagttcggc ctggccggtt acgtctggac gggggacgtg ggcagatcgc accggcttgc
     8041 gcgcgacctg gatgcgggca tgatctgggt caactcggaa aacaaccgtc atctgccgac
     8101 cccgttcggc ggcatgaagg caagcggcat cggccgggac ggcggtgact acagctttga
     8161 tttctacatg gaaaccaaga acgtgtgcat atccctcggc aagcaccgtg tgcccagcct
     8221 gggcaagggg tgaggctacc gtgagtctct actacgtgca gaagcttctc taccagctga
     8281 atcgggaccc tcacgcccgc cgacgctacg aggaggattt cgatgagctg ctggccgagt
     8341 acgacctcga cgccgaggag ctccacgcca ttcgggagcc cgacatcggg cttctctacg
     8401 tgatgggcgt gaacggccag atcctcatgc actacgccgc gctgcgcggc tacgcgtggg
     8461 acgagtacat caaggccatg cgtgacggcc ttgccgagca cggagacgtc cgggccgggc
     8521 tttacgtgac cacggacggc aggggggccg tctgatgggg gtcgtgtttg ccggcgtggt
     8581 cagccacgct ccggggatca cggggcgcgc agagcgcgcc gatcccgcgc tccgggacgc
     8641 cctgtacgaa aatttctacc gcctggggcg cgagctgaag gagtcgcgcc cggatgcgct
     8701 gatcgtcatc gccgccgagc acttcgccaa cttcttcatg gacaacatgc ccgcctactg
     8761 catgggaatg gccgattact acgaagggcc catcgaagac gagcagtggc tgggcatcag
     8821 tcgggtccgg attccgggca acgccgcgct ggctggcagc ctgatcgaat ccatgctggc
     8881 ggagttcgat ctggcctacg ctcaggagtg gaagttcgat cacggcgtca tggtcccgct
     8941 gcactttctc accccggagt acgacattcc ggtgattccc gtgaacgtca actgtcaggg
     9001 gccgccgctg acgccgctgg cgcgggcata cgatttcgga cgtgctttga ggcgggcttg
     9061 cgatgcgcgc ccggaacgca tcgccatcat cggcaccggc ggcatctcgc actggccggc
     9121 gacgcctgac tcgggaaaga tcaacgaagc ttgggatcgc gccttcctgg aaagctggct
     9181 ggccaatgac cgggcggcgc tcacctcttt cacggacgag gagacctatc tcgaggccgg
     9241 gcagggcggg ttcgagatcc gcaccttcat cgccgtttcg ggagccacac aaggatgtgc
     9301 ggcggaactc tacttctacg agccgatccc gatattcgcg gtgggttgca ccgtagcaag
     9361 ggttcacgtg tgacgacctg ctgccgcttc gcgctccccg tgtggccata ggcgggatga
     9421 cgggaaatag attgagcacg ccttcccaat gggtgtaggc tggctggcga acttgtcacg
     9481 acggataacc aggaggggcc atggctctgc agaacatcaa acccatcaac gatcagcttg
     9541 ccgacatcat cgtccgggcg gacgagccct ggatcgaaca ggagcccggc atggcctgga
     9601 taaaggtgct ctggacaggg gccgagaccg gccgatgggt ggctctgttc cgctggaaca
     9661 agggctacac cgcagcgcct cacaagcatc tgtccgacgc gcatacgtac gtgttgaagg
     9721 gcaagctgca ggtgcgtgac ggggtgctga acgcgggcga ctacgactac gagcccaacg
     9781 gcgttctgca cggcgcgacc acggccctgg aggacacgga gtacctgttt gtctgcgaag
     9841 gcccaattgt cttcttcgac gaaaacggcc tgacgaacta cctgagctgg gaagagctgg
     9901 cccgcatgcg cgatgcggcc aaggccggcg gcgacgcttc catgggtatc tccgtcacgg
     9961 ctgagtcctc ggcaggctga atctctgctg tgcccgcgcc gtaaagcggc gcgggcgctc
    10021 cggcctggat cagcccccgg gcagccgttc gaactcaggc accccggcag gaacggtgtg
    10081 gaacgtctgc ttgtccatgc agaagatggc cacctgaggc cccggatact ggccggggtc
    10141 atccatggag ccgaccttca ggatcaccgc atccggcatc gcgttggccc gcgtcagcag
    10201 gtgggttccg cagttgccgc agaactcccg ggtgacggga ttttcgatgt ccgtgcgggt
    10261 aaacgccgac ggttcgccct tggtgtactc gaatccggac ctgggaacgg ccatgaacat
    10321 gtttggcgcg ccgccggtga ggtactggca ctcacggcaa tggcattgcg cgctgaagac
    10381 cgggtcgccc tccgctttgt agcgcacggc tccgcagtaa catccgcctt ccaccgtcat
    10441 gggacctctc ctgtgattga ttggcttccc ttacgagtgt aacgcgcccg gcggataagt
    10501 gcctcctcca ccagcctggc agggccgaga cctctgggcc tctgcggacg ccgagggctg
    10561 aaactacgct acctcatcct ccacggtgtt tctcagcgtg ccgatgccgt ccaccgctac
    10621 ctcgaccacg tcaccgggct ttagatagcg tggcgggtcg aatctcgctc ctgcgcctgt
    10681 tggggttccc gtggagatca ggtcgcccgg ctgcagccgc atgaacgttg acaggtagct
    10741 gatcagatac tcgaaggaga agagcaggtt gtcggtgcgg tcgtcctggc gtagctcgcc
    10801 gttgaccttg gtggtcaggt gcagatcgtc gaaccgttcg atctcgtcga gggagacgag
    10861 ccacgggccg acggatccgg agcgttcgaa attctttccc tgagtaacgt tgaacttggc
    10921 gtgtctcagc caatcgcgga tcgttccctc gttcatgatg gtcaagccgg ccacgtgagc
    10981 gcgcgcctga ctctccggta tgcgtcggcc cgctttgccg atgacgatga cgatctctcc
    11041 ctcgtaatcg agctgctcgg actccggcgg tcgcacgatc ggttccaggt gacctacgag
    11101 agattcccgc gtgcgcagaa aaaggctcgg gtagcgggcg gccggagcgg cgtcggcata
    11161 ctcctcgttg cgattaccgt agttcacgcc gatgcagcag atcttctgcg ggtcgggaat
    11221 gggcggcaga aagccaacgt cttggagccg atggtcggga tcggcgtcct cggccacccg
    11281 gcgggcctgt tccagcgcgc cggctgcgag caggtcgcgc agggagccga aggccggcag
    11341 acgctccgcg agattcacga tgccgccgtc gatgaccgcg ccgaaagcgg tccttccctc
    11401 caatcgatat gtcaaaaatc gcacgcgctc tccccgtctg gcgcctatga gcatagaatg
    11461 tcagcgattc cctgatccgg agcgctgtca tacttcaccc tgattcctgc agtgtcaacg
    11521 cgaggctctc ccggtgcccc atctgatcat cgagtactcg gcgaacatcg acgaagagct
    11581 gatgcttgac gcgctcatgc tccggctgcg tgacagcgcc gtcgctacgg gcgtgttccc
    11641 cctgccaggc atccgcgtgc gcgcagcccg acgggaccgc ttcgtcgttg ccgatgggga
    11701 cccggaaaat gccttcgtgc acctcatggt caggatcggt catggccgga cgccggaggt
    11761 tcgccggcag gtcgcggatc gtctgttcgc ggtcttgtgt gatcacctca gagaggcggc
    11821 tacccggcgc gggctggggc tgtccctgga gatccaggag attgacccgg tgggaagcct
    11881 gaagcggaac aacctgcacg aacggctggc cgggcgcgcc gcaggccacg tctccgtcaa
    11941 ggtcgaatga agatcgggcc ggttctgatc tgcgccttcg cgctggcgat ggcccccttc
    12001 gccggcgcgc aaagcgtcag cgcctgggat ctggtttacc ccagcaattt caactgggag
    12061 atccgtactc accgggtgcg ggtggccaac gatctggcct cccgcgtgtc gctgctcgtg
    12121 gcggtggtgc cacggcagac ggaagaagag tcggcgcgat tgcgcgaaac cgagcagacg
    12181 ctggaggcgc gcggagccga tgcgtcccca agggatcgga gcaggctcta cctctcgcgc
    12241 gcctaccagc atcggcggct gctggagttg cttgcagata cgctggatgc gctgcagtgc
    12301 gttcgatcgg cagaagagtt gcccagggag gttcactgct ggacccgggc ggccgtcaat
    12361 ctcatggacg aggagaccgt cgacgtggcg ctcacggtgc tcagagatgc ccgcatggtg
    12421 cctcgggacg aggacatgcc ggtgaaggct caggacccga aggtctggta cggcgagtac
    12481 ggcaagggca tcgtgcggca catcgtgatg ccctatctgg ccggtctcgc ggcgcctcga
    12541 acgccggcgg ccacggaaga ggaagtcagc catgatggtt gagcgtgccc ccccgatcat
    12601 gttgactttg ctgctctcat cgacgctggc ggctgcctcg gagacggtgc tcttcaacga
    12661 gtcggccgcg ttcgagtgct accaggcggc cctgcacgac ggcggcgagt atgacatcga
    12721 gtcgtgcagc cgggctctgg agtatcaggc gctgaccacc atcgacaggg cggccaccca
    12781 ttccaaccgc ggcattctct acgcccgcat cggcgacatc cgcaacgctc ttcgggacca
    12841 caaccgcgcg gtgaagctcg ccccggaggt gggcagcatc tacatcaacc gttccaacac
    12901 cctggtccgg gcgaagcgct acgagggcgc catgcgcgac ctcgagaagg cgatcgagat
    12961 tgccgacgac agtctggctt acgcccatta caaccgggcg ctgctctttc agaagctcgg
    13021 tgatcttcag gcggcgaggg cggatgccga gcaagccgcg gagatagcgc cggactcgcc
    13081 ggcgtaccgg cgcttccttg ctgccttcgg cgaaaccgag ggcgacccgc tcctcccgga
    13141 tgccgagcca ggcaatcctc cggaggaacc gggcggtgag ggtcaggact gaatcaggcc
    13201 cttgagccgg cgcgcggttt cctcgggcga gcccgttcgc cgggcgagca gatgatagaa
    13261 agccggaacg atgaagagtg tcagcagcgt tgccagggag acgccggaga aaatgacgac
    13321 cccgagcatg ctgcggctcg tcgagccggc accggtcgcg aggatcagcg gcgtcgatcc
    13381 catgaccgtg gagatggtcg tcatgatgac ggggcgcagg cgaatgcccg ctgcttccac
    13441 gatggcgtcg tcgaaagaac ggcctgcatc gcgcagctga ttgatgaact cgacgataag
    13501 aacgccgttc ttggcggcaa tgccgatgag catgacgatg ccgatctggc tgtagatgtt
    13561 gagcgtgccg ccggtcagct tgaggccgag cagcgcgccg gcaatcgcca tggggacggt
    13621 cagcaggatg accagcgggt ggacgaagct ctcgaactgg gcggccagca ccagatagac
    13681 gatgagcagc gcgatggcga acgtgaacag aagtgatccg ctggcttcct tgaactccag
    13741 cgattcgccc cggtagtcca cctgggcgga ttccggcagc tcctcacgca ctacggtctc
    13801 cagaaactcc agagcgtccg ccagggcgta gcctggggcc aaaccggcct cgatggtcac
    13861 ggcgcggagc cggttgtagc gattgaggcg cccagctccg gcggtctcct ccacctcgat
    13921 gaggttggcc agcggaacca gacggccggt gctttccgag cgaacgtaga tgttctgaag
    13981 atctgcggcc gtcgcccgct gatcctcctt ggcctgcagg atcacgtcgt actcttcgcc
    14041 gtcctgcacg aaggtcgtga ccctgcgttc gctcatcatg gccgcaaggg ttctgcctac
    14101 gttctgcacg gagatgccca gcgccgccgc gcgattcttg tcgacccgca cgatcagctg
    14161 cggctgggtt tccttcaggt cgctgtcgac gcgcgtcatg ccggggtact ccatggcgcg
    14221 tgcgattaga agatcgcgcc attgggccag ggtctcgtag ttgggcccgc cgatgacgaa
    14281 ctgaaccggg cggccaccgc cgcctcggga cagaccggag cgctcgaagg cgaagatccg
    14341 cacggcggga atttcctccc acgctgtgga ggcccggtcg atgatctcgc gtgtggtggc
    14401 gtcccgttcc cgccacggct ccagggagac gatcaccatg ccggtgttcg ggctcgttcc
    14461 gccccagctg gggacccgga ccagcactct cgagatcagg ccctcgtcct gcatctgcat
    14521 caagggcgcc tcgacgccgc ccatgtgttc ctgcatgcga tcgaaaccgg ttccctccgg
    14581 cgccagcacc tgagccatga agaagccctg gtcctcccgg ggagcgtatt ccctgggcac
    14641 catgcccatg agccaatagg cgaggccgat gacgagcaag gtgatgacgg cggccgccca
    14701 gctgtagggg agaagacgtt tgagcaggcg ctgatagcct gcctccagcc gggcgaaggc
    14761 gcgctccacc atccgggtta ccgggttctt tgccgtcgtt ttgcgtagaa gcttggagct
    14821 catcatgggc gtcagcgaca gggccaggac gctggagaag accacggcgg cggaaaccgt
    14881 gacagccagc tcgctgaata tcacgcccat gttgtcgcgc aggaacatga tgggcgtgaa
    14941 cacggccacc agcacgacgg tggtcgcgat caccgcaaag gccacctgcc tcgaaccccg
    15001 gtacgcggcg acgagcggag gttcgccacg ctccacccgg cgatggatgt tctccagcat
    15061 gacgatggca tcgtccacga ccagcccgat gcacagcacc agggccagga tggtgaccag
    15121 gttgaccgag taaccgaaca gggccagggc gatgaatgcc gacgtcagac acacggggat
    15181 ggtcacggcg gggatgatca tcgagcggat gctgccgaga aagacgaaga tcacgaggct
    15241 gaccagcacc gtggtgatgg cgatggtctg gtacacggcg ttgatcgcct cgcgaatgaa
    15301 cagcgaatcg tccgagctag gcacgaactc catgtgatca ggcagcgcct cgttcacgcg
    15361 cgcgatctcg gctttgacgg cttccagcgt ggcgagcgtg ttggctgtgg attgcttggt
    15421 gatggccagg ccaacggtgt tctggcgatt ggcccggaac gactctctgg tgttgctggg
    15481 cgcgaggttc acgtcggcga cctcacccag gcgaatcaga tgcccgtctc cacccacgac
    15541 cagcaccagc tgtcgaaaat cctcgtcgct ttggtaaccc cgggccaggc gcactcggaa
    15601 ctcgcgctct gcggactcga tgcgtccggc gggaagctcg aggttctctc gccgcagagc
    15661 atcttcgata tcgatcacgg tcaggtcgcg ggcggccagc gcaagccggt ccagccatat
    15721 ccgcatgctc ggtttgccgc tgccgtgcat ggccgcgctg gcgacgccgg gaatgatttc
    15781 gaagcggtcg acgatgtagc gctccgcgta gtcgttgagc tccatcacgc tcattcgcga
    15841 gctggtcagg ctgatataca tgacgggacg ggcgtccgag tcctgtttgg cgacgatggg
    15901 cagctcgacg tcgtcaggca gccgatcgac gatgcgggcc accctgtccc ggacgtcgtt
    15961 ggccgcctcg tctatgttgc ggttgagctc gaactcgatg ttgatgttgg aggcctcgtc
    16021 acggctcgac gagctgatgc tctttatgcc ctcgatgccg ctgatctgat cctcgatcac
    16081 ctgagtgacg cgggattcga tgacctcggc cgacgcgcct acgtaagcgg tgctgatgga
    16141 aaccagcggg gggttgatgt cgggatactc gcgcagaggg agcaggttga aggacagagc
    16201 gccgaacgcg atcagggcca ggctgatcac gctggcgaac accggcctct gaacggagat
    16261 gtcagaaagc cacatgatgc tcagccctgt tcggcgcgaa ccgtcgtgat gggagagcca
    16321 tcgcgcacct tgatgaggcc gtcggtgatg acgcgctcac cttccgtcaa gccctcccga
    16381 acctccaccc agccggcgtc gcgtacgccg tgagtgatca gcctgctgcg ggcggtgccg
    16441 tcgacctcgg tgaagacgcg cacttgcgta ccgcgctgaa gcagcgctgc ctcgggaatc
    16501 atgagcacct cccgctcgga ggtggtcagc ctggccgtga gcagcatgcc cgggcgcagg
    16561 cgcatgtccc cgttgtcgat atgcgcccgc accacggcag cgcgcgtgac gggatcgacc
    16621 cgggagccga tggtgcttac cgtcgcgtcg aacaccgacc cggaataggc ggggctttcc
    16681 gcctctattc gctggccggg ttggaggcgg ctcaggtgaa tctccgggat ggagaagtcg
    16741 agcttgatga cggagacgtc gtccagggtc gtgatcgggg ttccgggggt gatcagcgtg
    16801 ccggtgctta cctggcgaaa ccccagaaca ccggagaagg gcgcggtgat cagccggtcc
    16861 tcgagacggg cgaccaccga ctggtagcgc gcgacggcgg ccgagtggcg cgcgcgagcc
    16921 tcgtccacct gagagatcgg cacgctgccc tgaaggacca ggtcttccag ccggtccagc
    16981 tgggttcggg tatccctggc gttggcctct gcttctgcca gcagagccgt ttcctcctcg
    17041 ttggtgagct ccaccagcac gtcgcccgcg ttgacgtagt cgccgtcctc gaaacgcacc
    17101 tggctgacgc tgtccgtt
//
LOCUS       DBNHLM010000351         9421 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1251046, whole genome shotgun sequence.
ACCESSION   DBNHLM010000351 DBNHLM010000000
VERSION     DBNHLM010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9421)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9421
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1251046"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="AC16QF_16150"
     CDS             <1..468
                     /locus_tag="AC16QF_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450143.1"
                     /translation="GPADPVFDSIGQFQGWLVPVDVSGIARSERGPEWTMSAGFIFNF
                     NLDQMGSLTLSGDWQWSDDAGIGGSRVSEPDGVLQYNGDFIKHQRDSYNILNASATWR
                     SARERYQVSAFVKNITNELYNQATTVVGGLLNFRNTNIRRHYAVEFKMNLGSI"
     gene            590..1807
                     /locus_tag="AC16QF_16155"
     CDS             590..1807
                     /locus_tag="AC16QF_16155"
                     /inference="COORDINATES: protein motif:HMM:NF025134.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HIR6450144.1"
                     /translation="MPKRDQRRARQQAARREKVRKQKARAKTTAGKRSKPPVDVTPHN
                     LPEAMTAAVALHREERLREAAVIYRRVLAIEPNHPDALSSLAMIEHQAARYPDAIEML
                     TRAIAIDGANPGYHMNLAAVQDAARNQREAEACYQRAIEIEPNYPDPYYNLGDLYLRQ
                     GKPDQAIDVFDACMAAIGREFHALAYKAHALDDAGRHEEARYLLNFDDYVKTYAFEPS
                     DGFESIADFNAALAAHIKTHPTLQGNVMSTEHGEHTGELLREPVGPMGAMEIRIHEAV
                     RWYIGQLPDDPQHPAVRWVPKAWKLTTWGVVMFDRGHERAHIHPNGWLSGVFYLELPD
                     LIADPARKPEGWLEFGRPTTDLHVQSELTLRHYQPAYGQMFLFPSYFYHGTVPFRSDQ
                     ERVCEAFDVEPLY"
     gene            complement(1823..2464)
                     /locus_tag="AC16QF_16160"
     CDS             complement(1823..2464)
                     /locus_tag="AC16QF_16160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6450145.1"
                     /translation="MSEEPEDVQIHLDSVDHVLSTARSVRRKLDFSRPVDPEVIFDCI
                     NVSTQAPTGLGGESWRFLVVTDLGRKREIAGLYRDVLVGLQQERGLVIKPTQQALVDR
                     LHEIPAMIFVCSMGAPPGPEIPTQVAYYGSVLPSAWSLMLSLRARNLGATWTTLLSSQ
                     QEALGRILDVPEDVVFTVMLPVGYMKGARLKKADRMDARRVTFWNRWGNDAPH"
     gene            2523..4517
                     /locus_tag="AC16QF_16165"
     CDS             2523..4517
                     /locus_tag="AC16QF_16165"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR6450146.1"
                     /translation="MNNEPLGDTVEGETMSELTHLFQPIQIGTMNLRNRIMMPPHGGF
                     IGDLWGSEEAAEQNLAYWARRARDGVAWIDGITGFVDNTLMVPGFLPTGLGAITRGVF
                     RMPHFKERAGRYADVIHEGGACATSQLVLQGGMPHGPSARLANYTNNSVPHVLDKDEI
                     RWLVEEYAYSARQIADAGLDGLELHANHEDLLELFLSPATNHREDEYGGDLNGRLRFI
                     KEILEAIRAEVGNSITVGVRLNMDEFFEGGYDLDEGLEIAKGLDATGHVDYFHGVIGN
                     NWGAPSYIQPHHYGLANWSEMAGRFKAAVGVPVVYAGRVTTPEAGEAVLAAGHADVVG
                     MARAMFADGEIVSKSKAGRLDEIRPCIGCNDCLHAGVVEGLPFGCTVNPRTGYEHKPP
                     PAPASPPKNLLVVGGGPAGLELAAAAAERGHTVTLWERDDALGGQMRLAAQVPENRAF
                     GDFIEFQERRLARLGVTVETGREATAESIAEAGFDVVAIATGATPRGHEASGAHLPFV
                     LEGRDVMSGEAKPGKRVAVIAMEDHLQPLTVASFLADRGHEVQLIYQTPSIAPLIGKY
                     SIGAPLAKITASGARVRVMERVTSIEQGRIETANVYSGAGSEITDFDSVVLACGGVAD
                     SGLYHSLKGKVDELHVLGDAYAPRRIWFATRQAYALAATL"
     gene            4557..5438
                     /locus_tag="AC16QF_16170"
     CDS             4557..5438
                     /locus_tag="AC16QF_16170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6450147.1"
                     /translation="MKFGLSMFTTDYSASPTEVAKAAEANGFESFWVSEHSNIPVDTD
                     FPMADTVPREYSSMLDPFVALAAAATVTERINLGTAICLVTQRDPINCAKLVASIDQL
                     SGGRMLFGIGAGWNEPEMRNHGTDPNTRFRLMRERVEAMKALWTNEVAEYHGRLVNIE
                     PTWQWPKPVQQPHPPVIVAGAGPNVLKRVIAYGEGWLPVVTPVAPEQMKGRVTPMDEF
                     VEMAGELERMADAAGRPTPAITVSGMNLDAATFDAFAELGVERMILRLTTAPLDDVLA
                     QIEQHVETVRSAGGSLH"
     gene            complement(5496..6503)
                     /locus_tag="AC16QF_16175"
     CDS             complement(5496..6503)
                     /locus_tag="AC16QF_16175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018926267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6450148.1"
                     /translation="MMSDTNRQFLLAARPVGMVKESDLQLHEAPVPEPGEGEVLVRVE
                     YISLDPSMRGQMENRADYVAPLALGDVMRARGVGRVVRSNDPEFPVGAVVSGTFGMQE
                     YAIGSDRVDRFRRWDDAVDPKVVLGTLGGTGLTAYFGLLDVGEPKSGDMVVVSGAAGA
                     TGSVAGQIAKIKGCTVIGMAGTDEKCRWLTDELGFDGAINYKTQDVAAELDRLCPDGI
                     DVYFDNVGGEILDICLDRIALNARVVLCGGISRYNVEGELPGPKNYFNLIFRRARMQG
                     FLAPDYAPRFDEAYAELQGWLAEGKIRQRETVIEGFENLPKALVLLFEGANTGKMMVK
                     V"
     gene            complement(6605..7750)
                     /locus_tag="AC16QF_16180"
     CDS             complement(6605..7750)
                     /locus_tag="AC16QF_16180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012336.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6450149.1"
                     /translation="MSDVAIVGIGMHPFGRHEGVTGMEQGASAVREACRDAGVEWKDI
                     QFAFGGSSAAGAPDTMVSQLGLTGLQFINVQNGCATGGSALFSAYNTIMSGAYDLGIA
                     IGFDKHERGAFRIQSGSGKGRDWYGGSGMALTTQFFGMKINHYMQQYGISQSTLAKVA
                     SKAFKNGSMNPMAWRRQALSEEEVLQSQMLSYPLTQYMFCSPGEGGVALILARADQAH
                     KYTKTPVYLKAAVVRSRRFGSFEVLAPSLALEHNAGPTIDASKAAFEIAGIGPEDVDL
                     AQLQDTESGAEVMHMAENGFCEHGEQEHIIQNGETEIGGRFPVNTDGGCLANGEPVGA
                     SGLRQVYENVLQLRGGAGERQVPGNPKVAYTHVYGAPGISGVNILTT"
     gene            complement(7762..8187)
                     /locus_tag="AC16QF_16185"
     CDS             complement(7762..8187)
                     /locus_tag="AC16QF_16185"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6450150.1"
                     /translation="MTQVSINDNVLELSAEGPRLKGMRCKTCGNHVFPFQKGCPKCAG
                     TDVEPVDLDTRGTLWAWTIQGFPPKAPPYLGEADPEKFKPYGVGYVEIPGQTKVEARL
                     TESDPTKLKEGMEMELTVEPISKDEDGNDIVTYAFKPVE"
     gene            complement(8204..9340)
                     /locus_tag="AC16QF_16190"
     CDS             complement(8204..9340)
                     /locus_tag="AC16QF_16190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6450151.1"
                     /translation="MVLVLSEEQELLRDSARDFAGNAIEVDWLRKVREQGAAGFDRDV
                     WRQMAELGWTAIPFAEEVGGLGLGYAEAGVVLEELGKALAVTPYLSSVVLGGGAVDLG
                     GSAEQKAEILPGVCDGTTLLTLAYQETARHDPYAIETSAVKTDGQWVLNGRKQLVLDG
                     GSADRIVVAARSSGAPGDRSGLTLFLVDAGAAGLDVKPGLLLDSRSAAHVALNDVTVG
                     EDAVIGEVDGGAEILDRVFERAAVALSAEMLGGIQEAFDRTVEYLKEREQFGAKIGSF
                     QGLKHRAARWFCEVELTRSIVMQALRAIDDGAENLAEIVSTCKARASDAFRLSGQEGI
                     QMHGGMGVTDEADIGFFLKRARVSELLLGDSAFHRNQFAELKGY"
BASE COUNT         1641 a         3256 c         2997 g         1527 t
ORIGIN      
        1 gggccggcag atccggtttt cgactcgatc ggccagttcc aaggctggct ggttccggtg
       61 gacgtttcgg gaatcgcccg cagtgaacgt ggacccgagt ggaccatgtc cgcaggcttc
      121 atcttcaact tcaacctgga tcagatgggc agcctgacgc tctccggcga ctggcaatgg
      181 tcggacgatg cagggatcgg gggctcgcgg gtgagcgagc cggacggcgt gctgcagtac
      241 aacggcgact tcatcaagca tcagcgcgat tcctacaaca tcctgaacgc gtcggccacc
      301 tggcgcagtg ccagggagcg ctaccaggtt tcggctttcg tgaagaacat cacgaacgag
      361 ctgtacaacc aggccaccac cgtggtcggg gggctgctca acttccggaa cacgaacatc
      421 cggcgccatt acgccgtgga gttcaagatg aatctcggaa gtatctgata gaggatcggt
      481 tcgcgaacgg gggcctcggc ccccgttttt ttagttgaac cgtcgggagg acgacaacca
      541 cccgccacgg atctatcatc cggcagatca attccgctgc agtgtcgaga tgcccaaacg
      601 ggatcaacgc cgggcacgcc agcaggcggc ccgccgggaa aaagtgcgca agcagaaggc
      661 tcgcgcgaag acgaccgctg gcaagcggtc gaagccgccc gtcgacgtga cgccccacaa
      721 tctgcccgaa gccatgaccg cggctgtagc cctccatcgc gaggaacgcc tgcgggaggc
      781 cgccgtcatc taccgtcgcg tgctcgccat cgagcccaac caccctgacg ctctgagcag
      841 cctggccatg atcgaacacc aggcggcgcg gtatcctgac gccatcgaga tgctgacccg
      901 cgcgatcgcc atcgacggcg ccaaccccgg gtaccacatg aacctggccg ccgtgcagga
      961 cgcggcacga aaccagcgcg aggcggaggc ctgctatcag cgcgccatcg agatcgagcc
     1021 taactatccg gacccctact acaacctcgg cgatctctat ctgcgccagg gcaaaccgga
     1081 ccaggccatc gacgtgttcg acgcctgcat ggccgccatc ggccgcgagt ttcacgccct
     1141 ggcctacaaa gcccacgccc tggacgatgc cgggcgccac gaggaagcac gatatctgct
     1201 gaacttcgac gattacgtga agacctacgc attcgagccg tcagacggct tcgagagcat
     1261 cgctgacttc aacgccgcgc tggcagccca catcaaaacc caccccaccc tgcaggggaa
     1321 cgtgatgtcg accgagcacg gcgagcacac gggcgagctg ctgcgcgagc cggtggggcc
     1381 catgggagcc atggagatcc ggatccacga ggccgtgcgc tggtacatcg gccagctgcc
     1441 ggacgatccg cagcatcccg ccgtgcgctg ggtgccgaaa gcctggaagc tgaccacctg
     1501 gggcgtcgtg atgttcgacc gcggccacga gcgcgcccac attcacccca acggctggct
     1561 gagcggcgtg ttctacctcg agctgccgga tctgattgcc gacccagccc gaaagcccga
     1621 aggctggctg gagttcggcc gccctacgac cgatctgcac gtgcagtccg agctgaccct
     1681 gcgccactac cagccggcct acggccagat gtttctgttc ccgtcctact tctatcacgg
     1741 gaccgttccg ttccggagcg atcaggagcg cgtctgcgag gccttcgacg tggagccgct
     1801 ctactgatcc agcaacagcg cttcaatgcg gcgcgtcgtt gccccagcga ttccagaagg
     1861 taacccgtcg ggcgtccatc cggtcggcct tcttgaggcg agcccccttc atgtagccca
     1921 ccggcagcat gacggtgaag accacgtctt cgggaacgtc gaggatgcgg ccgagcgctt
     1981 cctgctgcga ggagagcagc gtggtccagg tggcgcccag gttgcgggcg cgaagagaca
     2041 gcatcagcga ccacgccgag ggcagcaccg agccgtagta ggccacctgg gtaggtattt
     2101 ccggccccgg cggcgcgccc atggagcaga cgaagatcat ggccggaatc tcgtgcagcc
     2161 gatccaccag ggcctgctgg gtgggcttga tgacgagccc ccgctcctgc tgaagcccca
     2221 ccagcacgtc ccggtaaagg ccggcgattt cccgcttccg gcccaagtcc gtcaccacca
     2281 ggaagcgcca gctttcgccg ccaaggccgg tgggcgcctg ggtggagacg ttgatgcagt
     2341 cgaagatcac ctccgggtcc accggacggg aaaagtcgag cttgcgccgc accgagcgcg
     2401 ccgtgctcag cacgtgatcc acgctgtcga gatggatctg aacgtcttcg ggctcttcgg
     2461 acatgaggct acctccgcga tccatcgtgc cacgcgccgg ggttgtaatc gagccgcaat
     2521 tggtgaataa cgagccgctg ggggatacgg tcgaagggga gaccatgagc gagctcacac
     2581 acctgtttca gccgattcag atcggcacca tgaacctgcg caaccgcatc atgatgccac
     2641 cgcacggcgg gttcatcggc gacctctggg gcagcgagga agccgccgag cagaacctgg
     2701 cctactgggc ccgacgggcc cgcgacgggg tcgcctggat cgacgggatc acgggtttcg
     2761 tggacaacac gctgatggtt cccgggtttc tgcccacggg cctcggcgcc atcacccgcg
     2821 gcgtcttccg catgccccac ttcaaggagc gcgccggccg ctacgcggac gtcattcacg
     2881 agggcggcgc ctgtgccacc agccagctcg tcctgcaggg cggcatgcct cacggaccgt
     2941 cggccaggct cgccaactac accaacaact ccgtgcccca cgtgctggac aaggacgaga
     3001 tccgctggct ggtggaggag tacgcctact cggcccggca gattgcggat gccggcctgg
     3061 acggcctgga gctgcacgcc aaccacgagg acctgctgga gctgtttctt tccccggcca
     3121 ccaatcatcg ggaggacgag tacggcggcg atctgaatgg acgtctgcgc ttcatcaagg
     3181 agatcctgga ggccatccgg gccgaggtgg gcaactccat caccgtgggc gtgcgcctga
     3241 acatggacga gttcttcgaa ggcggctacg acctcgacga agggctcgag atcgccaaag
     3301 gcctcgacgc caccgggcac gtggactact tccacggggt gatcggcaac aactggggcg
     3361 cgcccagcta catccagccg caccactacg gccttgccaa ctggagcgag atggccggcc
     3421 gcttcaaggc cgccgtgggc gtgcccgtgg tctacgcggg cagggtcacc acgcccgaag
     3481 ccggggaggc cgtcctggcc gccggccacg ccgacgtggt aggcatggcc cgcgccatgt
     3541 tcgccgacgg cgagatcgtc tccaagtcga aggccgggcg tctggacgag attcgtccct
     3601 gcatcggctg caacgactgc ctgcacgcgg gcgtggtaga gggcctgcca ttcggctgca
     3661 cggtcaaccc ccgcacgggc tacgagcaca agccgccgcc ggcgccggcg agcccgccta
     3721 agaacctgct ggtggtgggc ggggggccgg ccggcctgga gctggcagcc gctgccgccg
     3781 agcgcggtca caccgtcacc ctgtgggagc gcgacgacgc ccttggcggg cagatgcgcc
     3841 ttgccgccca ggtgcccgag aaccgggcct tcggagactt catcgaattt caggagcgcc
     3901 gccttgcccg gctgggcgtc accgtggaga ccggccggga ggccaccgcc gaatccatcg
     3961 ccgaggcagg cttcgacgtg gtggccatcg ccaccggcgc cacgccccgc ggccacgagg
     4021 cctcgggcgc ccacctgccc ttcgtcctgg agggccgcga cgtgatgtcg ggagaggcca
     4081 agccgggcaa gcgggtggcc gtcatcgcca tggaggacca tctgcagccc ctcacggttg
     4141 ccagcttcct ggccgatcgg ggccacgagg tgcagctcat ctaccagacg ccgagcatcg
     4201 cgccgctcat cggcaagtac tccatcggcg ccccgctggc caagatcacc gcatccggcg
     4261 ccagggtgag ggtgatggag cgggtcacca gcatcgagca gggccggatc gagaccgcca
     4321 acgtctactc cggcgccggc tcggagatca ccgacttcga ctccgtggtg ctcgcctgcg
     4381 gcggcgttgc ggacagcggg ctgtaccact cgctcaaagg caaggtcgac gagctgcacg
     4441 ttctcggcga cgcctacgcg ccgcgaagga tctggtttgc cacgcggcag gcctatgccc
     4501 tggcagcaac cctctgaccg ttcggcgggc acgcttccga caaggagacc aacgccatga
     4561 aattcggcct gagcatgttc accacggact acagcgcgag ccccaccgaa gtcgcaaagg
     4621 ccgcggaggc aaacgggttc gagtcgttct gggtgtccga gcactccaac atccctgtgg
     4681 acacggactt tccgatggcg gataccgtgc cccgcgagta ctccagcatg ctggatccct
     4741 tcgtcgccct ggccgccgcg gcgacggtca ccgagcgcat caatctcggc acggccatct
     4801 gcctggtgac tcagcgggat cccatcaact gcgcgaagct cgtggccagc atcgaccagc
     4861 tctccggcgg gcgcatgctg ttcggcatcg gcgcgggatg gaacgagccg gagatgcgca
     4921 accacggcac ggatccaaac acgcgcttcc gcctgatgcg ggagcgggtg gaggccatga
     4981 aagcgctgtg gaccaacgag gtcgccgagt accacgggcg tctcgtgaac atcgagccca
     5041 cctggcagtg gccgaaaccc gtgcagcaac cccacccgcc cgtcatcgtt gccggggcag
     5101 ggcccaacgt cttaaagcgg gtgatcgcct acggcgaagg ctggctgccc gtggtcacgc
     5161 ccgtggcacc cgagcagatg aagggccgcg tgacgcccat ggacgagttc gtggagatgg
     5221 ccggcgagct ggaacgcatg gcggacgcag ccgggcgccc cacccccgcc attacggtct
     5281 cgggcatgaa cctggatgcc gccacgttcg atgcgttcgc cgagctggga gtggagcgga
     5341 tgattctgag gctgaccacc gcccccctgg acgacgtgct cgcccagatc gagcagcacg
     5401 tggagacggt gcgctccgcg ggaggatcac tgcactgaaa cgtcgacgcc cgggtgccgg
     5461 ggccgggagg aacgaccggc gacggctgag cggcgctaga ccttcaccat catcttgccg
     5521 gtgttcgcac cttcgaaaag cagcaccagc gctttcggca gattttcaaa gccttcgatg
     5581 acagtctcgc gctgcctgat cttgccttcg gcaagccagc cctgcagctc ggcgtaggcc
     5641 tcgtcgaagc gcggggcgta atccggggcc agaaagccct gcatgcgcgc ccgccggaag
     5701 atcaggttga agtagttctt cggccccggc agctctcctt ccacgttgta gcgggagata
     5761 ccgccgcaga gcacgacccg ggcgttgagc gcgatccggt ccaggcagat gtcgaggatc
     5821 tcgccgccca cgttgtcgaa gtagacgtcg atgccgtccg ggcacagccg gtcgagctct
     5881 gcggccacgt cctgagtctt gtagttgatg gcgccgtcga agccgagctc gtcggtgagc
     5941 catcggcatt tctcgtccgt gcccgccatg ccgatgacgg tgcagccttt gatcttcgcg
     6001 atctggcccg ccacgctgcc ggtggcgccg gccgcgccgc tgacgaccac catgtctcca
     6061 gacttcggct cgcccacgtc aagcaggccg aagtaggccg tgagccctgt gccaccgagg
     6121 gtccccagca ccaccttggg gtccacagcg tcgtcccagc ggcggaaacg gtccaccctg
     6181 tcactgccga tggcgtactc ctgcatcccg aacgtgccgc tcaccacagc gcccaccggg
     6241 aactccggat cgttggaccg caccacccgg ccaacccccc gggcccgcat gacgtcaccg
     6301 agagcgagcg gggcgacgta gtcggcgcgg ttctccatct gcccgcgcat ggaggggtcc
     6361 agagagatgt actccaccct caccagcacc tccccctcgc cgggctccgg aacgggtgcc
     6421 tcgtggagct gcagatccga ctccttgacc atgcccacgg gacgggcggc cagcaggaac
     6481 tgccggttcg tgtcactcat cagcgccctc ccacggtttc cagggcagac ggtgctgccc
     6541 cggatggccc gtggcgcggg cgcttaagag cgcgtcgtgc gcgttgagga cagtcatctt
     6601 tcgactacgt cgtgaggatg ttcaccccgc tgatgccggg cgcgccgtac acgtgggtat
     6661 aggcgacctt gggattgccg ggcacctgcc gctcgcctgc accgccgcgc agctgaagaa
     6721 cgttctcgta aacctggcgc agacccgagg cccccaccgg ctcgccgttg gccaggcagc
     6781 cgccgtcggt gttcaccggg aaccgcccgc cgatctccgt ttcgccgttc tgaatgatgt
     6841 gctcctgctc gccatgctcg cagaagccgt tttccgccat gtgcatgacc tcggcgccgg
     6901 actccgtgtc ctgcagctgg gccaggtcca cgtcttccgg accgatgccg gcgatctcga
     6961 aggcggcttt ggacgcatcg atggtcggcc cggcgttgtg ctcgagcgca agggacggcg
     7021 ccagcacctc gaagctgccg aaacgccgcg agcgcaccac ggccgccttc aggtagacgg
     7081 gcgtcttcgt gtacttgtga gcctgatccg cacgagcgag tatcaatgcc acgccaccct
     7141 cgcccgggga gcagaacatg tactgggtca gcgggtagga gagcatctgg gactgcagca
     7201 cctcctcctc ggacagcgcc tggcgccgcc aggccatggg gttcatggag ccgttcttga
     7261 aggccttgga agcgaccttg gcgagcgtgc tctgcgagat gccgtactgc tgcatgtagt
     7321 ggttgatctt catgccgaag aactgggtcg tcagcgccat gccgctgccg ccgtaccagt
     7381 cacgcccctt gccggagccg ctctgaatcc gaaacgcccc gcgctcgtgc ttgtcgaagc
     7441 cgatggcgat cccgagatcg taagccccgg acatgatggt gttgtaggcg gagaagagcg
     7501 ccgacccgcc agtcgcgcag ccgttctgga cgttgatgaa ctgcaaaccc gtgagaccga
     7561 gctgcgagac catcgtgtcc ggcgcgccgg ccgccgagct gccgccgaag gcgaactgaa
     7621 tgtccttcca ttcaacgccc gcatccctgc aagcttcgcg gaccgctgac gcaccctgct
     7681 ccatcccggt cacgccctcg tgccggccga atgggtgcat gcctatgcca acaattgcta
     7741 cgtcgctcat ggtttctctc cttactcgac cggtttgaat gcgtaggtga cgatgtcgtt
     7801 accgtcttcg tctttgctga tgggctccac cgtcagctcc atctccatgc cctccttgag
     7861 cttcgtcggg tccgactcgg tcaaccgggc ctcgaccttg gtctgcccgg gaatctcgac
     7921 gtagcccacg ccatagggtt tgaacttttc cggatcggct tcacccagat agggaggggc
     7981 cttgggcgga aagccctgaa tcgtccaggc ccacagcgtg ccgcgggtgt ctagatccac
     8041 gggctccacg tcggtgcccg cgcacttcgg gcagcccttc tgaaacggaa aaacgtggtt
     8101 gccgcaggtc ttgcagcgca tgcctttgag ccgcggccct tccgccgaca gctccagcac
     8161 gttgtcgttg atggaaacct gggtcatgtc acctccagtc gggctaatag ccctttaact
     8221 cagcaaattg gttgcgatgg aaggccgagt ccccgagcag cagctccgaa acccgggcgc
     8281 gcttcaagaa gaagccgatg tccgcctcgt ccgtcacgcc catgccgccg tgcatctgta
     8341 tcccttcctg gcccgaaagc cggaaggcat ccgaagccct cgccttgcag gtgctcacga
     8401 tctcggcgag gttttcagcg ccgtcatcga tggcgcgcag cgcctgcatg acgatggagc
     8461 gggtcagctc cacctcacag aaccagcggg ccgcgcggtg cttcaagccc tgaaaggaac
     8521 cgatcttcgc gccgaactgc tcgcgttcct tgagatactc gacggtccgg tcgaaggctt
     8581 cctggatgcc tccgagcatc tcggccgaga gcgccaccgc cgcgcgctcg aagacccggt
     8641 cgagaatctc ggcaccaccg tcgacctcgc cgatcacggc atcctcgccc acggtcacgt
     8701 cgttcagggc cacgtgagcc gcgctgcggc tgtcgagcag caggcccggc ttcacgtcga
     8761 gcccggcggc gcccgcgtcg accaggaaca gggtcagccc cgaccgatcg ccgggcgccc
     8821 cgctcgacct tgccgcgacc acgatccggt ccgcgctgcc gccgtccaga accagctgct
     8881 tgcggccgtt gagcacccac tggccatcgg ttttcacggc gctcgtctcg atggcgtagg
     8941 gatcgtgacg ggcggtctcc tggtaggcga gcgtcagcag cgtcgtgcca tcgcagacgc
     9001 cgggaaggat ctcggccttc tgctcggcac tgccgccgag gtctacggcg ccgccgccta
     9061 gcaccacgct ggacaggtag ggcgtcaccg ccagcgcctt gccgagctcc tcgagcacca
     9121 cgccggcctc cgcgtagccg aggccgagcc cgccgacctc ttcggcaaac gggatcgccg
     9181 tccagccgag ctcggccatc tgccgccaga cgtcccgatc gaaaccggcg gcgccctgtt
     9241 cccgcacttt ccgcaaccag tcgacctcga tggcgttgcc ggcgaaatcc ctggcgctgt
     9301 cccgcagcag ttcctgctct tcgctgagta ccagaaccac ggcggctaat ccggcaggcc
     9361 aagcacgcgc ttggcgatga tgttcatctg aatctccgag gtcccgccca ggatggtggc
     9421 c
//
LOCUS       DBNHLM010000352         3549 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1429867, whole genome shotgun sequence.
ACCESSION   DBNHLM010000352 DBNHLM010000000
VERSION     DBNHLM010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1429867"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="AC16QF_16195"
     CDS             complement(<1..413)
                     /locus_tag="AC16QF_16195"
                     /inference="COORDINATES: protein motif:HMM:NF028441.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HIR6450152.1"
                     /translation="MSRPIIVCLVILILLPWGLRSAAAVDLRLDGLTAEQSGRAIFEA
                     ADQLEDGYADMQVDLEMILRGRGGSEVHRQLRIKQLEVPEDGDKLLVVFDTPKPIKGT
                     ALLSVTHKRAPDDQWLYLPAMKRVKKIASQNKSGP"
     gene            581..1180
                     /gene="lexA"
                     /locus_tag="AC16QF_16200"
     CDS             581..1180
                     /gene="lexA"
                     /locus_tag="AC16QF_16200"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR6450153.1"
                     /translation="MTNLTPRQAQVLDLIKQHISDTGYPPTRADIAQELGFKSPNAAE
                     EHLKALSRKGAIEMIPGTSRGIRLPEETGLPVIGRVAAGNPILAEENIEDHVDIPSGF
                     FHPRADYLLRVRGDSMIDAGILDGDLLAVHKTPEAQNGQIVVARVEDEVTVKRFQRGR
                     NRSRVTLLPENSNYAPIEVDLQAQPFAIEGLSVGVIRRR"
     gene            complement(1188..1412)
                     /locus_tag="AC16QF_16205"
     CDS             complement(1188..1412)
                     /locus_tag="AC16QF_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450154.1"
                     /translation="MVFEITQLVDGDVVLREADDEGEPLVRIRFSAEVKDMLGDDAVV
                     VAEAMIDAAAAFLEADPEDQDLTAPAPGLH"
     gene            complement(1472..>3549)
                     /gene="topA"
                     /locus_tag="AC16QF_16210"
     CDS             complement(1472..>3549)
                     /gene="topA"
                     /locus_tag="AC16QF_16210"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR6450155.1"
                     /translation="AGRVQSVAVRLVVEREREIRAFIPEEYWEAFADLKRGADGDVLR
                     FQLIGDGGSNFRPDNGEDAHEALGRLKDRELVVRSREDKPTRTKPGAPFITSTLQQAA
                     STRLGFSVKKTMTLAQRLYEAGFITYMRTDATNLSADAVAACRDYIGEQHGAEYLPDQ
                     PRTYASKEAAQEAHEAIRPSNVRTLAANLSGVDDDAVRLYDLIWRQFVACQMPEARYL
                     STTVVVEAGELELRVRGRIMQFDGYTRVQPPSSRREEDVNLPDYAIGEKLALVHAEAV
                     QHFTKPPPRYGEASLVRELEKRGIGRPSTYAAIISTIQDRGYVTLRNRRFHAEKIGEL
                     VTDRLLEKFENLMDYGFTAQMEGELDRIAEGEIGWQAVLDEFYGDFSDKLVRAQSADD
                     GMRRNEPSDTDIKCARCGRVMQVRTASTGVFLGCSGYNLPPKERCTNTMNLIPGEEAV
                     DVEQDAEAESKLLRQKLHCPRCGTAMTAYLIDEARKLHVCGNNPDCAGFVVEAGQFRI
                     KGYEGPVIECDKCGADMQLKSGRFGKYFGCSSDECSNTRKLLRNGEPAPPKADPVPMP
                     ELRCEKVDDHYLLRDGASGIFLAASQFPKNRETRAPLVREIKPHGNELDPKFNYLLDA
                     PEEDPEGRPTVIRYSRKSREQYVQSELDGKATGWKAFYRDGRWVEENQPSTRSRKSAA
                     RKSSKARAG"
BASE COUNT          597 a         1194 c         1078 g          680 t
ORIGIN      
        1 aacggtcccg acttgttctg gctggcgatc ttcttgactc gtttcatggc aggcaggtag
       61 agccactggt cgtccggggc gcgtttgtgg gtgacgctga ggagcgcggt tcccttgatg
      121 ggtttcgggg tgtcgaaaac caccagcagc ttgtctccgt cctccggcac ttccagctgc
      181 ttgatccgca gctggcgatg cacctccgat cctccgcggc cgcgcagaat catctccaga
      241 tccacctgca tgtcggcata cccgtcctcg agctggtctg cagcctcgaa tatggctcgg
      301 ccggactgct cggcggtcag accgtcgagg cgcagatcca cggccgccgc ggagcgcaag
      361 ccccacggca gaagaatgag aatgactaga cagacgatga tggggcggct catgaactcc
      421 tcttccgctc cttcgcaaga ggccccccgc ggcccacggg gcttccgggg gatggacggc
      481 ggctcggata ttaagtaacc cccggggacc gtgcaaaccg ctcggatact gtatatactc
      541 acagcccact gtatattcaa ccaggctcga ttgaccgcaa atgaccaacc tcaccccaag
      601 acaggctcag gttctcgacc tcatcaaaca gcacatctcg gacaccggct acccgccgac
      661 ccgcgcggat atcgcccagg aactgggctt caaatccccc aacgccgccg aagagcatct
      721 caaggcactc tcgcgcaagg gcgccatcga gatgattccc ggcacctcac gcgggatccg
      781 gctgccggag gagacggggc ttcccgtgat aggcagggtt gcggcgggca acccgattct
      841 tgccgaggag aacatcgagg accatgtgga cattccgtcc gggttcttcc acccccgcgc
      901 cgactatctg ctgcgcgtgc gcggcgacag catgatcgat gcgggcattc tcgacgggga
      961 tctgctggcg gtgcacaaga cgccggaagc ccagaacggc cagatcgtcg tcgcccgagt
     1021 ggaggacgag gtcacggtga agcgcttcca gcggggccgg aaccgcagca gggtgacgct
     1081 gctgccggag aattcgaact acgcgcccat cgaggttgat ctgcaggctc agcctttcgc
     1141 catcgaaggg ctcagcgtgg gggtgatccg cagacgctga ctcggcgtca gtgcagaccg
     1201 ggagccggcg cggtgaggtc ctgatcttcg ggatccgcct ccaggaaagc ggcagccgca
     1261 tcgatcatcg cctccgcaac caccaccgca tcatccccca gcatgtcttt gacctcggca
     1321 gagaaccgga ttcgcacgag gggttctccc tcgtcgtccg cctctctgag gaccacgtct
     1381 ccatcgacga gctgcgtgat ctcaaacacc attccagatt agctccctgt gcgtctgaat
     1441 ttgctcgaac tctgcccgat gctcaacgca gctaacctgc gcgggctttg ctcgacttgc
     1501 gcgcggccga cttgcgcgac cgggtcgacg gctggttttc ctcaacccac cgaccatcgc
     1561 gatagaaggc tttccagcct gtcgctttgc catccaactc ggactgaacg tactgctcgc
     1621 ggctcttgcg gctgtagcgg atgacggtcg ggcgcccttc cggatcctct tccggcgcgt
     1681 cgagcagata attgaacttc ggatcgagct cgttcccgtg gggcttgatt tcccgtacca
     1741 gcggcgcgcg cgtttcccgg tttttgggaa actgactggc ggcaagaaaa atgcccgacg
     1801 ctccgtctct gagcaggtag tggtcgtcca ccttctcgca gcgcagctcg ggcatgggca
     1861 ccggatccgc cttgggcggc gccggctcgc cgttgcgcag cagcttgcgg gtgttgctgc
     1921 actcgtcgct gctgcacccg aagtacttgc cgaaacggcc cgacttgagc tgcatgtccg
     1981 cgccgcactt gtcgcactcg atcaccgggc cctcgtagcc ctttatgcgg aactggccgg
     2041 cctccaccac gaagccggca caatccggat tgttgccgca cacgtgcagc ttgcgggcct
     2101 cgtctatcag gtaggcggtc atggcggtcc cgcacctggg gcaatggagc ttctgccgga
     2161 gcagcttgga ttcggcctcg gcatcctgct cgacgtccac ggcctcttcg ccgggaatca
     2221 ggttcatggt gttggtacag cgctccttcg gcggcaggtt gtaccccgag cagcccagaa
     2281 acacgcccgt gctggcggtt cgaacctgca tcacgcgccc gcagcgcgcg cacttgatgt
     2341 cggtgtctga cggctcgttc cgacgcatgc cgtcgtccgc gctctgagcc cgcacgagct
     2401 tgtcgctgaa atccccgtag aactcgtcca gcacggcctg ccagccaatc tcaccctcgg
     2461 caatgcggtc gagctcccct tccatctgcg cggtgaaacc gtagtccatc aggttttcga
     2521 acttctccag caaccggtcg gtcacgagct cgccgatctt ctcggcatgg aacctgcggt
     2581 tccgcagcgt cacgtacccg cgatcctgaa tggtggagat gatggccgcg taggtggaag
     2641 gccgcccgat cccacgcttt tcgagctctc gtaccaggct ggcttcgccg tatcgcggcg
     2701 gcggcttggt gaagtgctgc acggcctccg catgaaccag agcgagcttc tccccgatcg
     2761 cgtagtcggg cagattgacg tcctcctccc tgcgcgaaga cggcggttgc accctggtgt
     2821 agccgtcgaa ctgcatgatc cggccacgaa cccgcagctc cagctcgccg gcctctacga
     2881 caaccgtggt gctgagatag cgggcctccg gcatctggca cgcgacgaac tgcctccaga
     2941 tcaggtcgta caggcgcacg gcgtcgtcat cgactccact gagattggca gccagcgttc
     3001 gcacgttgga cggccggatg gcttcgtgag cctcctgcgc ggcctccttg cttgcatatg
     3061 tgcgcggctg gtcgggcaga tattcggcgc catgctgctc gccgatgtag tcgcgacagg
     3121 cggccacggc atcggcgctc agattggtgg cgtccgtgcg catgtaggta atgaagcctg
     3181 cctcatagag cctctgggcc agcgtcatgg tctttttcac gctgaacccg aggcgcgtgc
     3241 tggccgcctg ctgaagggtg gacgtgatga agggcgcgcc gggcttggtg cgggtaggct
     3301 tgtcctcacg gctcctgacc accagctccc ggtctttgag acgccccagc gcctcgtggg
     3361 catcctcgcc gttgtccggc cggaagttgc tgccgccatc gcctatcagc tggaatctga
     3421 gcacatcgcc gtcagccccg cgcttgagat cggcgaaagc ctcccagtac tcctcgggga
     3481 tgaaggcccg gatttcccgc tcccgctcca cgaccagccg cacggccacc gactgcaccc
     3541 gaccggctg
//
LOCUS       DBNHLM010000353        10565 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1308580, whole genome shotgun sequence.
ACCESSION   DBNHLM010000353 DBNHLM010000000
VERSION     DBNHLM010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10565)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10565
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1308580"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..598
                     /locus_tag="AC16QF_16215"
     CDS             116..598
                     /locus_tag="AC16QF_16215"
                     /inference="COORDINATES: protein motif:HMM:NF013777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6450156.1"
                     /translation="MEDQPNEVLMRGLLRAYYWMDESLQNGLQKAGYLPRTRTQTMIL
                     INISNGVTRAAELARVLGVSRQAIQQQINELERDDLVTQLPDPDDRRANRIVFSERGS
                     QLINAALATLRQSEQALAIRLGYETVNQLRRALMADWGPVIGERRAPGRKRSISNQND
                     "
     gene            complement(699..2555)
                     /locus_tag="AC16QF_16220"
     CDS             complement(699..2555)
                     /locus_tag="AC16QF_16220"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6450157.1"
                     /translation="MLVALFSSGVTLAADHASRIEAHVESSATPWTGLQANDAEEDFT
                     FVVVADRTRGHRPGVFESAMPKVNLLQPAFVVSVGDLIEGYTDDPARLTGEWDEVEAF
                     VDQLDAPFFYAPGNHDMSNAVMSEAWRARFGPSFYHFLYKDVLFLVVNSELFGMVTNP
                     WVSVPGPWTQEEQMSYIERVLSRHADVRWTIVITHQPLWDVAEVHPDWLKVEALLGER
                     PYTVFAGHAHRYQRSLRHDRKYITLATTGGASPLRGALYGEFDHVAMVHMSSRGPTIA
                     NLMLDGIQPEDVTTEDSLRALRQLSDAVQVETEYLDGDLFTSGAVRYRIVNSGSAPLE
                     ATPIVLGNDVLRAQATPQAISVPPGGERRVALNLKSTHALAFESLPPARVTWSLRSML
                     LDRPVFVEIETALLPQRRFPLERISRLVRLDGDLEEWHLPYSVTRQGDIASPQTDPAD
                     ISFRFGIGYDDQNLYLAAEVIDDSIVASGELTAMDQDALIVSVDARPDPDRSANQPLV
                     EAMASGATSRMAIELLTLAPGRPAPVLEMLDGVRDAIASAVVRTSGGYAAEIAIPGEL
                     LSRQQGREWQVVRVGIHANDFDESERGSKSLHWQPYRFGSAAVVGTGTFMRR"
     gene            complement(2728..2991)
                     /locus_tag="AC16QF_16225"
     CDS             complement(2728..2991)
                     /locus_tag="AC16QF_16225"
                     /inference="COORDINATES: protein motif:HMM:NF014609.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIR6450158.1"
                     /translation="MQLTIVDVFAERRYAGNQLAVVRDASGLSTAEMQDIAREMNFSE
                     TAFVLESGSGHARARYFTPPPVRLGGEFDRALAGASSSIRAWR"
     gene            3121..3573
                     /locus_tag="AC16QF_16230"
     CDS             3121..3573
                     /locus_tag="AC16QF_16230"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR6450159.1"
                     /translation="MTGTVEGIYLAAKHGDSQRAVPAARLTIGVGLEGDRHANSKGGV
                     VTLIEAEAVERFIAETGLDITAADTGRNIATRGIGLNDLVGRQFRVGGVLMEGFELCE
                     PCATLGARLASRDVSAAAIVAAFTHSGGIRAFVRGTGTVAPGSAVEPA"
     gene            complement(3583..4173)
                     /locus_tag="AC16QF_16235"
     CDS             complement(3583..4173)
                     /locus_tag="AC16QF_16235"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6450160.1"
                     /translation="MTRQSGADGYPKQVRLPGGEEIEIRLMTGEDRDAVCEFARGLAE
                     EDLMFLRVDLTEPAVIDQWIRNLETGHSTSIVAYDGTGLVGYATVHRDPARWTRRVGE
                     IRVNVSPAYRAKGLGRTLTSQIFDLARSLGLRKLMANMTVDQSGAQAAFRRLGFVPEA
                     LLADYVEDRSGTPRDLVIMSYDIEGHTDQVAEPLRV"
     gene            complement(4193..5698)
                     /locus_tag="AC16QF_16240"
     CDS             complement(4193..5698)
                     /locus_tag="AC16QF_16240"
                     /inference="COORDINATES: protein motif:HMM:NF014998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR6450161.1"
                     /translation="MRLTSHDASFLYSESASGPMHTASIFVLEGELPFERVFSHFQAR
                     MHLIARYMQKLAFVPFNLAHPKWVDDPDFDLANHVVPHSVPEGSTLEEAVDLLLAVNE
                     GLLDRNRPLWKTYVVDGVPGKTLMLQQVHHAMIDGVSGIQLTMVQFDFDRDAPDPPPG
                     EPRQPATPPSAMDLVGEVVRESAERLSVNPLAGVHTDRKSFELLRNAGRAFSRFFTEP
                     AITAPWNAGLVGPKRRLRWTSHPFSEFREIRRAFGGTINDVVLAMVSEGAARYLDRRR
                     EETEGKYLRIMCPVSVRTEDEAGSLGNRVSAIFPLLPAWSMDAAERLRAVIGETERIK
                     RDEEAQALTFLSETMPSVPAVAMAPTLLVGTRFDPTALAGRMPLPVVPNLGTRLPLYG
                     FNFTCTNVPGVQVPQYLAGHEISNIIGILMLTGTLGYGMAVVSYNQNMRFNFVCEPRL
                     MPDLDVMVEAVEQVYQELLEEARRVNEPPAPPSRGGRSEKASRKSYDSPAA"
     gene            5836..7500
                     /locus_tag="AC16QF_16245"
     CDS             5836..7500
                     /locus_tag="AC16QF_16245"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR6450162.1"
                     /translation="MRGGQVFMEGLVAHGADCIFGNPGTTENSLLDRLIDYPGLRYYV
                     ALHEGVAVGAASFYAQASGKTGIVNLHVAPGLGNGIGMIYGALKANSPLIITAGQQDT
                     RMRMREPLLAHDLVAMAEPVCKWSAEPRSADEIGPMLRRAFKIANEPPRGPVFMSLPV
                     DVMEQETSVGATTAGDLRYGTLADAEGIRRLARWLAGSANPAIVVGDDIAAEGAHRAL
                     ITLAVQTGAAVWQQGLRAQISFPNVHPNSKGRLPFEAGAIRRALAGHDLILLMGGPFF
                     EEIWFDAENALPEEARVVHVESSHARLALNLAPDLGLVGNLRTTIEHLVEEIAEGAPD
                     DFGAAAKARNDALAESHRAAGDAAADRIERQGDTSPMTPARALLEISRALPKGAIVVD
                     ESITASLEVASRFDYAAPGDFFAGSGGGIGQGLAGALGVQVAQPNRRVLAISGDGSAM
                     YSIQALWSAVHHGLPIVFVILSNREYRILKHNLDIYRQRFDAGSNKPYPHMDLTRPAL
                     GFPAMAEGMGMEGELVTRPEKIGSAIERAFAANAPYLVEVEISGKP"
     gene            complement(7624..9036)
                     /locus_tag="AC16QF_16250"
     CDS             complement(7624..9036)
                     /locus_tag="AC16QF_16250"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIR6450163.1"
                     /translation="MSLELINLWYIVAAALFIFGLKQLSSPDTAVRGNLLSSVGMLIA
                     VVVTLFSRDILEFQWIAAAAAVGAIIGAVAAQRVAMTSMPEMVALFNGSGGIASLLVG
                     WAALYLGDGAAAEAAVADTAVVTVGLSTFTIITILLSVLIGGLTFSGSLIAWGKLSEV
                     MTSRAIVFGAQRLVNAALLIGLVACGVMFAMEPSLTSPYLYAVIALSLIFGVMAVIPI
                     GGADMPVVISLLNSYSGLAACAAGFAINNNILIVAGSLVGAAGIILTNIMCKAMNRSL
                     ANVLFSGFGAVKTATKVEGEVKPITPDDAFLILEAASSVTFVPGYGMAVAQAQHVVRE
                     LGELLEENGAEVTYAIHPVAGRMPGHMNVLLAEANVPYDQLVEMDDINPRIDNIDVAV
                     VIGANDVVNPAAREDENSPIYGMPIINVDQARTVFVLKRSMASGFSGVDNPLFFGENT
                     RMLFGDAKASISSVIAEFKG"
     gene            complement(9033..9338)
                     /locus_tag="AC16QF_16255"
     CDS             complement(9033..9338)
                     /locus_tag="AC16QF_16255"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha part 2"
                     /protein_id="HIR6450164.1"
                     /translation="MEAVFLAFVLMLSIFLGFELISKVPATLHTPLMSGANAISGITL
                     VGAITSAGAGADQIATLLGAVAVGFATINVVGGYLVTNRMLSMFKRKDTGGQSGGRS"
     gene            complement(9351..10478)
                     /locus_tag="AC16QF_16260"
     CDS             complement(9351..10478)
                     /locus_tag="AC16QF_16260"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HIR6450165.1"
                     /translation="MLIGVPKETWPGEHRAALVPQSVKKLVGAGFEVVIEAGTGVPAG
                     YPDAAYVDAGATIGGDRAGLLASADVVLRVRKPEADEIAGLKKGSVHVSFLDPFNERG
                     LVDAMAAQGVTAISMEMIPRSTRAQKMDALSSQANLAGYVTVVLAANHCPKIFPMMMT
                     PAGTIAPSRVFVIGAGVAGLQAIATAKRLGARVEAFDTRPVVAEQVRSLGGKFVEIDL
                     GEVGQTEQGYAKELTPEQVELQKEGQKKVIGQSDVVITTAQVFGRPAPRIVSKDMVEA
                     MQPGSVVVDMAVETGGNVEGSVLNEVVDVGGVKIIGLGNLPSQVARHASDMYSSNLFN
                     LLDEFWNQEESKLDLDPEDEIVRACVITRDGSIVNETIKNL"
BASE COUNT         1946 a         3483 c         3364 g         1772 t
ORIGIN      
        1 ctgatctccc aatttataac agttgcttga cagcgcactt tgagaaacaa taccggatac
       61 tgcgtacctc ggaaacgggc cgcatgaatt gtgttgcgat tgattgagga ttgacatgga
      121 agatcaaccc aacgaagtgc tgatgcgcgg cttgctgcgc gcctactatt ggatggatga
      181 gagcctccag aacgggctgc agaaagcagg ctacctgcct cgcacccgca ctcagacgat
      241 gattctcatc aacatatcca acggcgtgac cagagctgcg gagctggcac gggtgctggg
      301 cgtgagccgg caggccatcc agcagcagat caacgaactg gagcgtgacg acctcgtcac
      361 ccagcttcca gatccggacg accggcgcgc caaccgaatc gtcttcagcg agcgcggttc
      421 gcagctgata aacgctgcac tggcaacact gcgccaatca gagcaggccc tggcgatacg
      481 gctgggctac gagacggtga accagctccg acgcgctctg atggcagatt ggggccccgt
      541 gatcggagag cggcgcgctc ccggacgcaa gcgctccatt tcgaaccaga acgactgacc
      601 ggcgcgccgc cttccggcgg cgctctcgct ccccccggac ctggcgtcgc tgaacgccgg
      661 gcgggtgcga ctccccccca acatcgtcgc cgagctgatc accttcgcat gaatgtgccc
      721 gtgccgacca cggccgccga gccgaatcga tagggctgcc agtggagcga cttcgagccg
      781 cgttcgctct cgtcgaagtc gttggcatga atgccgacgc gaacgacctg ccactccctg
      841 ccctgctggc gactgagcag ttccccaggg atggcgatct cggctgcgta tccgccggac
      901 gttcgcacca ccgcgcttgc aatggcgtcg cgcacgccat cgagcatctc cagcaccggt
      961 gccgggcgcc cgggggccag ggtcagcagc tctatggcca tgcgcgacgt ggcaccgctg
     1021 gccatggcct ctactagagg ctggttcgca gagcggtccg ggtcgggacg ggcatccacc
     1081 gagacgatga gcgcgtcctg atccatcgcg gtcagctcac cggaggcaac gatggagtcg
     1141 tcgattacct cggcagcaag gtaaaggttc tggtcgtcgt agccgatccc gaagcgaaac
     1201 gagatgtccg ccggatcagt ctgcgggctg gcaatgtcac cctggcgcgt caccgagtac
     1261 ggcagatgcc attcctcgag gtctccgtcc aggcgaacca gacggctgat gcgctccagc
     1321 ggaaagcggc gctggggaag cagcgcggtc tcgatctcta cgaataccgg ccgatcgagc
     1381 agcatcgacc gcaacgacca ggtgaccctc gccgggggca ggctctcgaa ggcgagcgcg
     1441 tgcgtggatt tcagattcag cgcgacccgc cgctcccctc cgggcggcac cgagattgcc
     1501 tggggagtcg cctgggcgcg cagaacgtcg tttcccagga cgatgggcgt cgcctcgagg
     1561 ggagccgacc ccgagttgac gatccggtag cgaaccgcgc ccgaggtgaa caggtctccg
     1621 tcgagatact cggtttcgac ctgcaccgcg tccgacagct gcctcaacgc acgcagcgag
     1681 tcctctgtgg tgacgtcctc gggttgaatc ccatcgagca tcagattcgc gatggtgggt
     1741 ccgcgcgagg acatgtgcac catcgccaca tgatcaaact cgccgtaaag agcgccccgg
     1801 agcgggctgg ccccgccggt ggtcgccagc gtgatgtact tgcgatcgtg gcgtaacgat
     1861 cgctggtagc ggtgcgcgtg accggcaaac accgtatacg ggcgttcacc gaggagcgct
     1921 tcgaccttta gccagtcggg gtgaacctcg gcgacgtccc acagcggctg atgggtgatg
     1981 acgatcgtcc agcggacgtc ggcatgcctg gagagcacgc gctcgatgta actcatctgc
     2041 tcttcctggg tccagggccc cggcacggac acccacggat tcgtcaccat gccgaacagc
     2101 tcggagttga cgaccaggaa cagcacgtcc ttgtagagga agtgatagaa ggacggtccg
     2161 aaacgggcgc gccaggcctc ggacatgacc gcgttggaca tgtcgtgatt gcccggcgcg
     2221 tagaagaagg gcgcatcgag ctggtcgaca aaggcctcga cttcatccca ctcccccgtg
     2281 agccgggccg gatcgtccgt gtaaccttca atgagatctc ccacgctgac cacgaatgcc
     2341 ggctgcagca gattgacctt gggcatggcg gactcgaaca cgcctggccg atgaccgcgc
     2401 gtgcggtcgg ccaccacgac aaaggtgaaa tcctcttccg catcgtttgc ctggagtccc
     2461 gtccacgggg tggctgagga ttcaacgtga gcttcgatgc ggctcgcgtg gtcggcagcc
     2521 agggtgactc ccgaggagaa caacgcgacg agcacaacca ggcccctcgc gggccagctc
     2581 gccctgaccg gcgggcggct cgcggacggc cgtcctgtct tctgccgacg cgaatctttc
     2641 aagccagtcg gccctctgcc accagttgca ccttgccgcc gacgcggatc tcgtctccca
     2701 cgtcgagata gagccgcgag ggccgtttca tctccacgcc ctgatcgacg acgacgcgcc
     2761 cgccagggcc cggtcgaact ccccgcccag ccgcacgggc gggggcgtga aatagcgggc
     2821 gcgggcgtgg ccgctgccgc tttccaggac gaaggcggtc tccgagaaat tcatctcccg
     2881 ggcaatgtcc tgcatctctg cggtggagag gccgctcgca tctcgcacca ccgccagctg
     2941 attaccggcg tagcggcgct cggcgaacac gtcgacgatg gtgagctgca ttcgagaacc
     3001 caaggcggca cgtttcgcca tcgagactag cacccgctct ctgcatttcc caacctccgc
     3061 ccatcaggcc gcgtgatgaa cttgcgctaa actcgctcat cagcaccgga gggcttcccg
     3121 atgacaggca cagtcgaggg catatatctg gccgcaaagc acggcgattc ccagcgggcc
     3181 gtgcccgcgg ctcgactgac cataggcgtc ggcctcgagg gtgatcgtca cgccaactcg
     3241 aaaggcgggg tggtcaccct gatagaggcc gaggcggtag aacggttcat cgccgagacg
     3301 ggcctggaca tcacggcggc ggacaccggt cgaaacatcg ccacccgcgg catcggcctg
     3361 aacgacctgg tgggcaggca gtttcgcgtc ggcggggtgc tgatggaagg cttcgagcta
     3421 tgcgagccct gcgccaccct gggcgcgcgg ctggcgagtc gggacgtgag cgcggcggcc
     3481 atcgtcgctg cctttacgca cagcgggggc attcgcgcct tcgttcgggg aaccggcacc
     3541 gtggcaccgg gaagcgccgt cgagccggcc tgatcgcatc tctcacactc gcagcggttc
     3601 ggcaacctga tccgtgtgac cctcgatgtc gtaggacatg atcaccaggt cccgcggggt
     3661 tccgctacgg tcctccacgt agtccgccag cagcgcctcg ggcacaaagc ccagccgacg
     3721 aaaggccgcc tgggcaccgg actgatccac cgtcatgttc gccatgagct ttcgcaagcc
     3781 gagcgaccgg gccaggtcga aaatctgcga cgtcagcgtc cggcccagcc ccttggcgcg
     3841 gtatgcgggg cttacgttca cccggatctc ccccactctg cgggtccatc gggcggggtc
     3901 gcgatgcacg gtcgcgtacc ccaccaggcc ggtgccgtcg taagccacga tcgaagtcga
     3961 gtgacccgtc tcgaggttgc ggatccactg atcgatcacc gcgggctccg tcaggtccac
     4021 gcgcagaaac atcaggtctt cctcggcaag cccccgggca aattcgcaaa ccgcgtcccg
     4081 gtcctctccg gtcatcaggc ggatctcgat ctcttcgccg ccgggcagcc gcacctgctt
     4141 ggggtacccg tccgcaccgc tctgtctggt catctttcta caccctcctc agtcaggccg
     4201 ccggcgagtc gtaggacttc cggcttgcct tttccgaccg gccgccccgg ctgggcgggg
     4261 ccggcggctc gttgacccgg cgcgcttcct cgagcagctc ctgatacacc tgctcgacag
     4321 cctccaccat cacgtccaga tcgggcatca ggcgcggctc gcagacgaaa ttgaagcgca
     4381 tattctggtt gtagctcacg actgccatgc cgtacccgag ggtgccggtg agcatcagta
     4441 tgccgatgat gttgctgatc tcgtgaccgg ccaggtactg cggcacctga acgcccggca
     4501 cgttggtgca ggtgaagttg aacccgtaga gcgggagccg ggtgcccagg ttgggcacga
     4561 ccggcaacgg cattcgtcca gcgagggcgg ttgggtcgaa gcgggttccc accagcagcg
     4621 ttggcgccat ggcgaccgca ggcacggacg gcatcgtctc gctgaggaac gtcagcgctt
     4681 gcgcctcctc gtcccgtttg atgcgttcgg tctctccaat cacagcgcgc agcctctcgg
     4741 cggcgtccat ggaccaggcc ggcagcagag ggaaaatggc ggacacacga ttgccgaggc
     4801 tgccggcttc gtcctcggtg cgcacgctca ccgggcacat gattctcagg tacttccctt
     4861 cggtttcctc gcggcggcga tccaggtagc gggcggcccc ctctgagacc atggcgagaa
     4921 ccacatcatt gatggtccca ccgaaggccc gtctgatctc tctgaactcg gagaacgggt
     4981 ggctggtcca gcggagccgg cgcttcggcc cgaccaggcc ggcgttccaa ggcgccgtga
     5041 tggcgggctc agtgaagaag cgcgagaacg cgcgaccggc attgcgcagg agctcgaagc
     5101 tcttgcggtc ggtgtgcacg cctgcgagcg ggttgaccga cagccgctcg gcgctctccc
     5161 gcaccacctc gccaacgaga tccatggcag agggcggcgt tgcaggctgc cgcggttctc
     5221 cgggcggagg atccggggcg tcccgatcaa agtcgaactg gaccatggtg agctggatgc
     5281 ccgaaacccc gtcgatcatg gcgtgatgca cctgctgaag catcagcgtt ttccccggca
     5341 cgccgtccac cacataggtc ttccaaagcg gtcggttgcg gtcgagcagc ccctcgttca
     5401 ccgcgagcag gagatccacc gcctcctcca gggtcgagcc ctcgggaacc gagtgcggca
     5461 ccacgtgatt ggcgagatcg aagtccgggt cgtccaccca cttcgggtgc gccaggttga
     5521 atggtacgaa ggccagcttc tgcatgtaac gcgcaatgag gtgcatgcga gcctgaaagt
     5581 gactgaacac acgctcgaac ggcagctcgc cctccagcac gaagatggaa gcggtgtgca
     5641 tcgggccgct ggccgattcc gagtacagaa acgaggcgtc gtgcgaagtc agtcgcatgg
     5701 gttcatccga tcaggggcgc acactatgcg cggagacgaa ccggctcgtc aacgcgacgc
     5761 cgcggctggg cggcctggct accggcgctg cgctgcctgt aacattcgac gctcggttta
     5821 cccagggaga aggcgatgcg cggtggtcag gtgttcatgg aggggctcgt cgcccacggc
     5881 gcggactgca tcttcgggaa ccccggcacc accgaaaatt ctctgctcga ccggctcatc
     5941 gactacccgg ggctgcgcta ctacgtcgcc ctgcacgagg gcgttgcggt gggcgccgcg
     6001 agcttctacg cccaggcgag cggcaagacg ggcatcgtga acctgcacgt cgctcccggg
     6061 ctcggcaacg gcatcgggat gatctacggc gcgctgaaag ccaactcgcc gctcatcatc
     6121 accgcggggc agcaggacac ccgcatgcgc atgcgggagc ctctgctcgc tcacgacctc
     6181 gtcgccatgg ccgagccggt ctgcaagtgg agcgccgagc cacgcagcgc cgacgagatc
     6241 ggccccatgc ttcggcgggc gttcaagatc gcgaacgagc cgccgcgggg cccggttttc
     6301 atgtccctgc cggtggacgt catggagcag gaaaccagcg ttggcgccac taccgcaggg
     6361 gatctccgct acgggacgct cgcggacgcc gagggcatcc gccgactggc gcgctggctg
     6421 gccgggagcg caaacccagc catcgtcgtc ggcgacgaca tcgccgccga gggtgcccac
     6481 cgggcgctga tcacactcgc cgtgcagacc ggggccgccg tatggcagca gggtttgcgg
     6541 gctcagattt ccttcccgaa cgtgcacccc aacagcaagg gacgcctgcc gttcgaagcc
     6601 ggcgccattc gccgcgcgct cgccggccat gatctgattc tgctgatggg cggcccgttc
     6661 ttcgaggaga tctggttcga tgcggaaaac gcgttgcccg aggaggcccg ggtcgttcac
     6721 gtggagtcca gtcatgcccg tctggcactg aacctggccc ccgatctcgg tctggtcggc
     6781 aacctgcgga caacgatcga gcatctggtg gaggaaatcg ccgagggcgc gcccgacgac
     6841 ttcggggcgg cggcaaaggc gcgaaacgac gcgctggccg aatcccatcg ggccgccggc
     6901 gatgccgccg cggatcggat cgagcgccag ggcgatacct cgccgatgac gccagccagg
     6961 gccttgctgg aaatcagccg cgcgctgccc aaaggcgcca tcgtcgtgga cgaatccatc
     7021 accgcatcgt tggaagtggc atcgcgattc gattacgccg cccccggcga cttcttcgcg
     7081 ggctcgggcg gcggtatcgg ccagggcctg gccggcgccc tgggcgttca ggtggctcag
     7141 cccaatcggc gcgtcctggc gatctccgga gacggctctg ccatgtacag cattcaggcc
     7201 ctgtggtccg cggtgcatca cgggctgccc atcgtgttcg tgatcctgtc gaaccgtgaa
     7261 tatcggattc tcaaacacaa tctcgacatt taccgccaac gcttcgatgc agggtccaac
     7321 aagccgtacc cgcacatgga tctcaccaga ccggcactgg gttttcccgc catggccgag
     7381 ggaatgggaa tggagggaga gctggtcaca cggccggaga agatcggctc cgccatcgag
     7441 cgcgcgttcg ccgcaaacgc gccctatctg gtggaggtcg agatctccgg aaagccctag
     7501 cgttggcaga gaaccctcca gtgcaacgcg atccggacca cgctgccggg tatcacgatg
     7561 cccaacctcg atgggttcat ctcggcggtg tcgggcgtgt ctacgccctg aaaggcctgt
     7621 cggctaccct ttgaactcgg cgatcaccga ggagatcgac gccttggcgt cgccgaacag
     7681 catccgcgtg ttctcgccga agaacagcgg gttgtccacg cccgagaacc cggaagccat
     7741 cgatcgcttg aggacgaaca cggttcgcgc ctggtcgacg ttgatgatgg gcatgccgta
     7801 gatcgggctg ttctcgtcct cccgggcagc cggattgacc acgtcgttgg cgccgatcac
     7861 gaccgcaacg tcgatgttgt cgatgcgggg gttgatgtca tccatctcga ccagctgatc
     7921 gtaaggcacg ttcgcctcgg ccagcaggac gttcatgtgc cccggcatcc gcccggccac
     7981 cggatggatg gcgtaggtca cctcggcacc gttttcctcg agaagctcac caagctcccg
     8041 aaccacgtgc tgcgcctggg caacggccat tccgtaaccc gggacgaacg taaccgatga
     8101 ggctgcttcc agaatgagga acgcgtcgtc cggcgtgatc ggcttgacct cgccctcgac
     8161 cttggtggcg gtcttgacgg caccgaagcc ggagaaaagc acgttggcaa gggaacggtt
     8221 catggccttg cacatgatgt tggtcaggat gatgcccgca gcgcccacca gtgaacccgc
     8281 gacgatgagg atgttgttgt tgatcgcaaa tcccgccgcg catgcggcaa gaccggagta
     8341 gctgttgagc agcgagatca ccaccggcat atcggcgcca ccgatgggaa tgaccgccat
     8401 tacgccgaag atgagcgaca gcgcgattac cgcgtagaga tagggtgagg tcagcgacgg
     8461 ctccatggcg aacatcacgc cacaggcaac gagaccgatc agcagagcgg cgttgaccag
     8521 gcgctgagcc ccgaagacga tggcccggct cgtcatcacc tctgacagct tgccccaggc
     8581 gatgaggctg cccgagaagg tcaagccgcc aatcagcacg gagagcagga tggtgatgat
     8641 ggtaaaggta gaaagaccca ccgtgacgac ggcggtgtcg gccactgcgg cctcggcggc
     8701 ggcgccatcg ccgaggtaga gggccgccca gccaaccagc aggctggcaa tgcctcccga
     8761 gccgttaaac agggcgacca tctccggcat gctcgtcatg gccactctct gcgcggcaac
     8821 ggccccgatg atcgcgccaa cggcagcagc cgccgcaatc cactggaact cgaggatatc
     8881 ccgggagaac agggtcacca ccacggcaat gagcatgccc acggatgaca gcaggttgcc
     8941 acgcacggcc gtgtccggcg agctgagctg cttgaggccg aatatgaaaa gcgcggcggc
     9001 gacgatgtac cagaggttga tcagttccag gctcatgacc gaccccccga ctgaccaccg
     9061 gtgtctttcc tcttgaacat ggacaacatg cgattggtga cgaggtaacc gcccaccacg
     9121 ttgatggtgg caaaaccgac ggcgacagcg cccagcagcg tcgcgatctg gtcggccccc
     9181 gcgcccgcgg atgtgatcgc gccgacgagg gtgatgcccg agatcgcgtt cgcgcccgac
     9241 atcagaggcg tgtgcagcgt cgcaggcact ttggaaatga gctcgaagcc caggaagatg
     9301 gagagcatca atacgaaggc gaggaaaacg gcttccacgg ttccggactc ctagaggttc
     9361 ttgatggttt cgttgacgat cgatccgtcc cgcgtgatca cgcaggcccg aacgatttcg
     9421 tcttccggat cgaggtcgag cttgctttcc tcctggttcc agaattcatc cagcaggttg
     9481 aagagattgc tcgagtacat gtcactggca tgccgcgcaa cctgcgacgg caaattgccc
     9541 aggccaatga tcttcacgcc gccgacgtcg accacttcgt tgagaacaga gccctcgacg
     9601 ttgccgccgg tctcgacggc catgtcgacc acgacgcttc ccggctgcat ggcctcgacc
     9661 atgtcctttg acacgatgcg gggtgccgga cgaccaaaca cctgcgcggt ggtgatgacc
     9721 acgtcggact ggccgatcac ctttttctga ccttctttct ggagctccac ctgctcaggc
     9781 gtcagctctt ttgcatagcc ctgctctgtc tgcccgacct cgccaagatc gatctcgacg
     9841 aacttgccgc cgagagagcg aacctgctcg gccaccaccg ggcgggtatc gaatgcttcg
     9901 actctagcgc cgagccgctt cgccgtggcg atcgcctgca gtccggcgac ccccgcgccg
     9961 atcacgaaca cccgcgacgg ggcaatcgtc ccggccggcg tcatcatcat gggaaagatc
    10021 ttggggcaat gattggcggc gagaaccacc gtgacgtatc cggcgaggtt ggcctgggat
    10081 gacagggcgt ccatcttctg cgcgcgcgtg ctgcgcggga tcatctccat ggaaatggcc
    10141 gtgacgccct gggccgccat ggcatcgacc agccctctct cgttgaaggg atccagaaag
    10201 ctgacgtgca ccgatccctt tttgagcccg gcgatctcgt ccgcttccgg cttgcgcacc
    10261 cgcaggacga cgtccgccga ggcgagcaga cccgcccgat cgcccccgat ggtcgcaccg
    10321 gcatcgacgt aggcggcgtc gggataaccc gcagggactc ccgtcccggc ttctatgact
    10381 acttcgaacc cggcacctac gagctttttg acgctctgag gaacgagcgc cgcacgatgt
    10441 tcccccggcc aggtttcctt gggtacccca atgagcatgc aatcccccca aatcgcagtc
    10501 gcgccggcat gaagaagaga gcggaagatg cggcgcagga aagcgcagca agttagccaa
    10561 acccc
//
LOCUS       DBNHLM010000354         6359 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_173667, whole genome shotgun sequence.
ACCESSION   DBNHLM010000354 DBNHLM010000000
VERSION     DBNHLM010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6359)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_173667"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="AC16QF_16265"
     CDS             <1..807
                     /locus_tag="AC16QF_16265"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6450166.1"
                     /translation="PSPFTLYGTGTYILGTGNVAVIDPGPADPAHIAAIVAGLQGETI
                     SHVLVTHTHMDHSPGCALLREHTDAPTYAFGPHGAGKLEEGVPVEEGGDMEFVPDVVV
                     RHGDVVEGADWSVECVYTPGHTSNHMCFQLREQKALFTGDHVMGWSTSIISPPDGDMF
                     AYMKSLDLLLERDDAVYWPTHGPCIDAPKTHVRAFIEHRREREEQILKCVSRGVGQIR
                     DMVPIMYVDTPEFMYPAAARSVLAAVEGLVARGDLACDGAVSLDGNYRLT"
     gene            complement(804..3005)
                     /locus_tag="AC16QF_16270"
     CDS             complement(804..3005)
                     /locus_tag="AC16QF_16270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6450167.1"
                     /translation="MKIFSRIKGPRLGTKIMLLGLTLLIVPWFSYRQLVEMERLLIQG
                     QSQAQLLTAEGISTLFNGREDLFNDLPVAVEDFENLYANPLQAAVRLDGRLDDWGSEI
                     PGKLMHFGSEKTGADADFELVLGERGGQLHVFMRVTDPDLIYRNPDYLRLDNADHVRL
                     SFIRADGEDGRILLTLPAPGLVTAYEMDADWRFAESGNPTNAVQGFMEPVDGGLQLEF
                     RLPLQLLGSSRYFGIAYVDVDDPALREIVHVTQTLPKAGLESFNLVVLRSPEVLNIIQ
                     GLGYSGARILVIDAQQRVRAETGSNRPIEETDPASPTFVLLRDGFEQVRPFLHWVSTG
                     EDYLPVKSRPEDSEGTADVAITTSLTGDPIALRRALSENNEIIIAAHPIVSRDAVIGT
                     VVVEQNIDDILSFQRSALEQVILLSIASLFAVFIALLAFAGRLAWRIRNLRQEASSAI
                     DQYGRLKISELHNEMKAGDEIGDLARSVSNMLSKLHQHNNFLENMPRTLRHEINNPLN
                     TLSTSLQNLAEENPEIQDSKYMESAKRGVNRIGSIVQNLADAANLEESLEAEELEVVD
                     IAQLLENYVANCRITHPTCEFVFRGPNRPVLSRVSDFRIEQLLDKIIDNAIDFHRSNS
                     PIVVQLDAYRDHLQITVANRGPILPAKAEKSLFDSMVSHRGQQNRLHFGLGLYVVRVI
                     ADHHGGFVRALNLADGSGVAIVVQLPTVETTEGTPGRQRDAEDIEPGVAAS"
     gene            complement(3021..3719)
                     /locus_tag="AC16QF_16275"
     CDS             complement(3021..3719)
                     /locus_tag="AC16QF_16275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6450168.1"
                     /translation="MAKTIAIVEDDPDQRANYADAITMKGYKVSAYGSRQEALEGFDQ
                     ALPDLVILDIILGEEVDAGFQLCRDLLARAPSLPVIFLTERINEIDKISGLRLGAWDY
                     LPKPISLDYLAERISSLLRINEVRAEQQETESATAKRIGDLMLDQDALLVSWKGHRID
                     LSGTEFRMLAKLVRMPGHAVSYETLMNATMQSLVTNNTINTHMRNIRKKFEKVDKDFS
                     SIKSEYGFGYRWSA"
     gene            3935..4117
                     /locus_tag="AC16QF_16280"
     CDS             3935..4117
                     /locus_tag="AC16QF_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450169.1"
                     /translation="MKVFIPFSEALVEESGFPAGELVPFQLEYECWRHLGENADCESA
                     GAASGQGTAQTVELPA"
     gene            4164..4772
                     /gene="pnuC"
                     /locus_tag="AC16QF_16285"
     CDS             4164..4772
                     /gene="pnuC"
                     /locus_tag="AC16QF_16285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HIR6450170.1"
                     /translation="MPEIFEQIIAMAWLESVGLGSGLLCVWLLIRQNIWTFPIGLVYS
                     VVSVGVFAHERLYADVLLSVYYVGMNAYGWYYWLYGGRRTVADLLPVTHTPRPTLLWL
                     AGVTALATFVMGWFFDTQTNASLPYWDSATTCMSFAAMWMTARKYIENWVVWLVVDVM
                     ATVIYLVKGIELYAVLYGIYLAMAIMGWRAWRRSLNLASSSA"
     gene            4733..5278
                     /locus_tag="AC16QF_16290"
     CDS             4733..5278
                     /locus_tag="AC16QF_16290"
                     /inference="COORDINATES: protein motif:HMM:NF024911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6450171.1"
                     /translation="MATEFEPGLVVCLTGPESTGKSTIAEQLASALAVPLVPEVAREY
                     LAGRPDYEQSDLLAIARAQLAAEQDALAEGTGLVVCDTDLQVIQIWWEERYAELDPWL
                     AQALVDRSPRRYLLAVPDLPWEPDPLRESPHDRERLFRRYRQVLREGAFPYAEVRGLG
                     RSRFDSALVTVQRWLAEPQQD"
     gene            complement(5280..6116)
                     /locus_tag="AC16QF_16295"
     CDS             complement(5280..6116)
                     /locus_tag="AC16QF_16295"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6450172.1"
                     /translation="MPGEDPDIGDIRRGICRLLPEWAPEELLDFDYLPGGYSNHNFRF
                     ARRGNQYVVRVPFERPGEQAVAGERSVYEALTDPSVPAVIALGDDGWLVSRWVEGPLL
                     VDAPPSAQTIVGFIRRLHESLRVRAAPYDPAARSRAYLETPGDSSPDARVMAIAHSLN
                     WRLDDTVACHNDLNPWNVIVSASGDWVTLDWQSVGANDPLFDLVTLHQGLSLPDAGLT
                     DLCEALLERPVAPERVERVLVGFWLREYAWAFAALQLGNRRQEILDQLALSRDRLASL
                     AG"
     gene            6177..>6359
                     /locus_tag="AC16QF_16300"
     CDS             6177..>6359
                     /locus_tag="AC16QF_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450173.1"
                     /translation="MEVTLSRFVHALRSAEVEVSPAETLDGFAVVRQVGIADPVLLQD
                     ALGLALAKTREEKARFA"
BASE COUNT         1064 a         1995 c         2099 g         1201 t
ORIGIN      
        1 cccagcccgt tcaccctcta cggtaccggc acgtacattc tgggcaccgg caacgttgcg
       61 gtgatcgatc ccgggccggc cgatccggcc cacatcgcgg ccatcgtcgc cggccttcaa
      121 ggcgaaacca tttctcacgt gctggtgacc cacacccaca tggatcactc cccgggctgc
      181 gcgctgctgc gtgagcacac cgatgcgcca acttatgcct tcgggcctca cggcgccggc
      241 aagctcgagg agggcgtccc cgtggaggag ggcggcgaca tggagttcgt gcccgacgtg
      301 gtggtccgtc acggtgacgt cgtcgagggc gccgactggt ccgtggagtg cgtctacacg
      361 cctggccaca cctccaatca catgtgtttc cagctgcgcg agcagaaagc tcttttcacc
      421 ggcgaccatg tcatgggctg gtcgacgagc atcatctcgc caccggatgg cgacatgttt
      481 gcctacatga aaagcctcga tctgctgctc gagcgcgatg acgccgtcta ctggcccacc
      541 cacgggccct gcatcgatgc gccgaaaacc cacgttcggg ccttcatcga gcatcgcagg
      601 gagcgggagg agcagattct gaagtgcgtg agccggggcg tcgggcagat tcgcgacatg
      661 gtgcccatca tgtacgtcga cactcctgag ttcatgtatc cggccgcagc ccgcagcgtt
      721 ctggctgcgg tggaagggct ggtggcccgg ggcgacctcg cctgcgacgg cgcggtctct
      781 ctcgacggca actaccgact gacttagctc gccgctaccc cgggctcgat atcctccgcg
      841 tcccgctgac gacccggcgt tccctccgtc gtctcgactg tgggcagctg aacgacgatg
      901 gcgactccgg agccatcggc caggttgagc gcccgcacga acccgccgtg gtggtcggca
      961 atgacccgga ccacgtacag gccgaggccg aagtgcagcc ggttctgctg tccccggtga
     1021 ctgaccatgg agtcgaaaag cgacttttcc gccttggcgg gaaggatggg gccgcggtta
     1081 gccaccgtga tctgcagatg gtcacgatag gcgtcgagct gaaccacgat tgggctgttg
     1141 gagcggtgaa agtcgatggc gttgtcgatg atcttgtcga gcagctgctc gatgcggaag
     1201 tcggataccc ggctgagaac cggacgattg ggcccgcgaa acacgaactc gcacgtcggg
     1261 tgggtgatgc ggcagttcgc cacgtagttt tccaggagct gagcgatgtc caccacttcg
     1321 agctcctcgg cttccagcga ctcttcgaga ttcgcagcgt ccgccagatt ctgcacgatg
     1381 gagccgatgc ggttgacgcc gcgcttggcg ctctccatgt acttgctgtc ctgtatctcg
     1441 gggttctcct cggccaggtt ctgcagggac gtgctgaggg tgttcagcgg gttgttgatc
     1501 tcgtgccgga gcgtgcgggg catgttctcc aggaagttgt tgtgctggtg cagcttggac
     1561 agcatgttgg aaacgctgcg cgcgagatcg ccgatctcgt cgccggcctt catctcgttg
     1621 tgcagctcgc tgatcttcag acgcccgtac tgatcgatgg ccgagcttgc ctcctgacgc
     1681 aggtttcgga tgcgccatgc gaggcggccg gcaaaggcga gcagggcgat gaagacggca
     1741 aacaggctgg caatggacaa caggatcacc tgctccaggg ccgaccgctg gaaggagagg
     1801 atgtcgtcga tgttctgctc caccaccacg gtgccgatga cggcgtctct ggagacgatg
     1861 gggtgcgcgg cgatgatgat ctcgttgttc tcgctcagcg cccggcgcag agcgatgggg
     1921 tcgccggtga gcgaggtggt gatcgccacg tccgccgtgc cttcggaatc ctccgggcgg
     1981 ctcttcacgg gcaggtagtc ctcgccggtg gagacccagt gcagaaaggg ccgcacctgc
     2041 tcgaagccgt cgcgcagcag gacgaacgtc gggctcgcag gatcggtctc ctcgatgggg
     2101 cggttgctgc cggtctccgc gcgaacccgc tgctgggcgt cgatgaccag gatgcgggcg
     2161 ccggaatacc ccaggccctg aatgatgttc agcacctcgg gcgagcgcag caccaccagg
     2221 ttgaagctct ccagccccgc cttgggcagg gtctgggtga cgtggacgat ctcgcgcagg
     2281 gccgggtcgt ccacgtccac gtaggcgatg ccgaagtagc gtgacgagcc cagaagctgc
     2341 agcggcaggc ggaactccag ctgcagcccg ccatccaccg gctccatgaa tccctgaacc
     2401 gcgttcgtcg ggttgcccga ctcggcgaag cgccagtcgg cgtccatctc gtaggcggtg
     2461 acgagcccgg gagctggcaa agtgagcaga atccgtccgt cctcgccatc ggcgcggatg
     2521 aagctcaggc gaacgtggtc ggcgttgtcg agccgcaggt aatcggggtt gcggtagatc
     2581 agatccgggt cggtgacgcg catgaagacg tgcagctgcc cgccgcgctc accgagcacc
     2641 agctcgaagt ccgcatcggc ccccgtcttc tcgctgccga agtgcatgag cttgccgggg
     2701 atctcgctgc cccagtcgtc aagccggccg tccaggcgca cggccgcctg gagggggttg
     2761 gcgtaaaggt tttcgaagtc ttccaccgca acgggcagat cgttgaacag atcctcccgg
     2821 ccgttgaaga gggtggagat gccttcggcg gtgagcagct gggcctgtga ctggccctga
     2881 atgagcagcc gctccatctc caccagctgg cggtagctga accagggaac gatgagcagg
     2941 gtgagcccga gcagcatgat cttggtgccg agccggggac ccttgatgcg actgaagatc
     3001 ttcatgggcg ttggcgagcg tcaggccgac cagcgatatc cgaacccgta ctcgctcttg
     3061 atgctcgaga agtccttgtc gaccttttcg aacttcttgc ggatgttgcg catgtgggtg
     3121 ttgatggtgt tgttggtgac gaggctctgc atggtcgcgt tcatgagcgt ttcgtagctc
     3181 acggcatggc ccggcatgcg aacgagcttt gccagcatcc ggaactcggt gccggacaga
     3241 tcgatgcggt gacctttcca ggacaccagc agcgcgtcct ggtcgagcat gaggtcgccg
     3301 atccgctttg ccgtggcgct ttccgtctcc tgctgctcgg cccgcacctc gttgatgcgc
     3361 agcagcgagg agatccgctc ggccaggtag tccaggctga tgggcttggg caggtagtcc
     3421 caggcgccca gacgaagccc cgagatcttg tcgatctcgt tgatccgctc ggtgaggaag
     3481 atgacgggca acgatggcgc gcgggcgagc aggtcccggc aaagctgaaa gccagcgtcc
     3541 acctcctcgc cgagaatgat gtccaggatg accagatcgg gcagcgcctg atcgaagccc
     3601 tccagtgctt cctgccggct cccgtaggcg gaaaccttgt agcccttcat ggtgatcgcg
     3661 tcggcgtagt tggcccgctg atcggggtcg tcctcgacga tcgctatggt tttcgccata
     3721 cctgtgtgcc tgcgcggcgg caaaatccgg gtggattcta gcccgttttg gaagttttag
     3781 cgcgaggagt gatccatgca cggtcactgg tccacagttt ctccatgatc atcaatcacg
     3841 actccacgtt ttctggcgga agcgtgggtt aagggcaggt ttaatgcttt agacctgcga
     3901 agagcagcac agcaaggatg ttttgcgctt agtcatgaaa gtcttcattc ccttcagcga
     3961 ggcgctcgtc gaggaatccg ggtttcccgc cggcgagctg gtgcccttcc agctcgagta
     4021 cgagtgctgg cggcatctgg gcgagaacgc ggattgcgaa tccgcggggg ccgcgtccgg
     4081 tcagggcacc gcccagaccg ttgaattgcc agcctagaac ctagtctcta tcgttgggcg
     4141 ctcctttacc gagcgtccat ccgttgccgg aaatctttga gcagatcatc gccatggcgt
     4201 ggctcgaatc cgtaggtctg ggttcggggc tgttgtgcgt gtggcttctc atccgtcaga
     4261 acatctggac ctttcccatc gggctcgtct actccgtcgt ctccgtcggc gtttttgctc
     4321 acgagcggct ctacgccgac gtgctgctca gcgtctacta cgtcgggatg aacgcctatg
     4381 gctggtacta ctggctttac ggcggcaggc gtaccgtggc cgatctgctt ccggtgaccc
     4441 acacgccgcg gcccacgctg ctgtggctcg ccggggtgac cgcgctcgcc accttcgtca
     4501 tgggctggtt cttcgacacc cagaccaacg ccagcctccc ctactgggac agcgccacca
     4561 cctgcatgag cttcgccgcc atgtggatga cggcccggaa atacatcgag aactgggtcg
     4621 tctggctcgt cgtggacgtc atggccacgg tcatctatct ggtcaagggc attgagctgt
     4681 atgctgttct ctatggcatt tatctggcaa tggccatcat gggctggcgg gcgtggcgac
     4741 ggagtttgaa cctggcctcg tcgtctgcct gacgggcccc gagagcaccg gcaagagcac
     4801 catcgcggag caattggcca gcgccctggc cgtcccgctg gtgccggagg tcgcgcggga
     4861 gtatctcgcc ggaagacccg actacgaaca gtccgacctg ctggccatcg cccgggcgca
     4921 gctcgctgcg gagcaggacg ccctggccga aggcaccggt ctcgtcgtgt gcgataccga
     4981 cctgcaggtc atccagatct ggtgggagga acgctacgcg gagctggacc cgtggctggc
     5041 ccaggcgctc gtcgaccgtt cgccccgtcg ctacctgctc gccgttccgg atctgccctg
     5101 ggagcccgac cccctgcgcg agtcgcccca cgaccgggag cggctctttc gacgctaccg
     5161 gcaagtcctg agggaaggcg cttttccgta cgccgaagtc cgcggtctgg ggcggtcgcg
     5221 gttcgattcc gcgctggtta ccgtgcagcg ctggctggcc gaaccccagc aggattgaat
     5281 cagccggcca gagaagccag ccggtcacgg gacagggcga gctgatcgag aatttcctga
     5341 cgccggttac caagctgaag ggcggcaaag gcccaggcgt actcgcgcag ccagaatccc
     5401 acgagcactc gctccacccg ctcgggcgcg acgggccgct ccagcagcgc ttcgcacagg
     5461 tccgtcagcc cggcgtccgg caggctcaaa ccctgatgca gggtcaccag atcgaacagc
     5521 gggtcgttgg cgccgacgga ctgccagtcg agggtcaccc agtcaccgct ggccgagacg
     5581 atgacgttcc acgggttgag atcgttgtgg caggcgacgg tgtcgtccag gcgccaatta
     5641 aggctgtggg cgatggccat cacccgggcg tccggtgacg agtctccggg cgtttcgaga
     5701 taggcacggc tgcgcgccgc aggatcgtaa ggcgctgccc tcacgcggag cgattcgtgc
     5761 agccgcctga tgaaaccgac gatggtctgc gctgacggcg gggcgtcgac gagtaagggg
     5821 ccttcgaccc agcgggatac cagccagccg tcatcgccca gggcgatcac ggccggtacc
     5881 gaagggtctg tcagcgcctc gtagacggaa cgttcgcccg ccacggcctg ctcgccgggc
     5941 cgttcgaagg gaacccgaac cacgtactgg ttgccccggc gggcgaagcg gaagttgtga
     6001 ttgctgtaac ccccgggcag gtagtcgaag tcgagcaact cttccggcgc ccactcgggg
     6061 aggaggcggc agatgccgcg acggatgtcg ccaatgtctg gatcttcgcc cggcaagcgc
     6121 aaggccctta aactcagcca tcggatggta gcccggcttg ctagaatcgg tcacccatgg
     6181 aggtcaccct aagccgcttc gttcacgccc tgcgcagcgc cgaggtcgag gtttcgcccg
     6241 cggagaccct ggacgggttc gccgtggtgc gtcaggtggg tattgccgat ccggttctgc
     6301 ttcaggacgc cctggggcta gcccttgcca agacccgcga agagaaagcg cgctttgcc
//
LOCUS       DBNHLM010000355         5831 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1550447, whole genome shotgun sequence.
ACCESSION   DBNHLM010000355 DBNHLM010000000
VERSION     DBNHLM010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1550447"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="AC16QF_16305"
     CDS             complement(<1..359)
                     /locus_tag="AC16QF_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043711 - pilus organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis chaperone"
                     /protein_id="HIR6450174.1"
                     /translation="MKTERFHICSIIVCSMVALATGSACAELVVNRAIVVFDSPSQHR
                     QDVELVNIDAGDNLFVDIESFQVERPGEDDQALTSLRGNENPGLIATPQKAMIGPSNS
                     QLVRFMNLDRDGDREKV"
     gene            complement(397..972)
                     /locus_tag="AC16QF_16310"
     CDS             complement(397..972)
                     /locus_tag="AC16QF_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450175.1"
                     /translation="MLARLRTASVNALCLGIALLVVVGGGSARGAQPNGIDGAFGETS
                     KAEARVTLRIRSGARIVRGPGTGGRPDAPPGGGRGRPDLNLSSRDLSETGEAVLPLCL
                     NAPSGQAFEVSALPDGGDEASGLRTASGSAVSYEVSLGAEGGASATFISSGRDCTPEN
                     SLRLSISTTRSAPDTELTNLTGHVRLFVVPV"
     gene            complement(978..1922)
                     /locus_tag="AC16QF_16315"
     CDS             complement(978..1922)
                     /locus_tag="AC16QF_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450176.1"
                     /translation="MTYAANIGNDNYLSVGGVPDVDFGTWSGGSLSTTVRGCIASANE
                     RQPNPPSPPAEVYPYQAGVDNRNPGSDFYLYLNNDASSPESARITVVVEHRDIVAGSA
                     FETLTEGVFDTHYHLGRFQECLGTGDNSELRISIPEANLTNKVAGVYHGQFRLEAVGG
                     TSGTKYDRTDFDLILTVQSANVRISGMDDMALGTYVPGTGNVRQDEHFCVQSLADNAA
                     YRISVSSSNQTPGGDFRLKHATDAVYLPVDVLFSSLGSGSGTEQITNNSISALGSPDS
                     YCGGSDNATITVRALEADVQAVPSGSYGDTLVLLVVPE"
     gene            complement(2108..2629)
                     /locus_tag="AC16QF_16320"
     CDS             complement(2108..2629)
                     /locus_tag="AC16QF_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450177.1"
                     /translation="MKKTSAIALTTLGLLAASSAMAATDGSYDTTSTGTTDVSLTVVD
                     ATRITGLDDIDLGTYAATDTDGVDASDTFCVHVNGGDAYNITATYGPLASPTTSDTIA
                     VQVSLIDNAAGTGAEDVNTGGVSSNYTGAALIDCGGSDNAYINVEVEDADIRAATTAS
                     DYATTVSLLVSPQ"
     gene            complement(3046..5004)
                     /locus_tag="AC16QF_16325"
     CDS             complement(3046..5004)
                     /locus_tag="AC16QF_16325"
                     /inference="COORDINATES: protein motif:HMM:NF012927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR6450178.1"
                     /translation="MKLLSPVEVGPIEVKNRVVSTAHAAFLEFFNPVSDGERYMAYQE
                     RRAQGGTGLIIFTAMHVHESSQIPYHYVFDADTMAPKFAQISTRLHRHGAKCISQLFH
                     FGAQGKSDPRDDFHPLWSFSGTTTLEGEVTHKMTDAEIEEVIDAFAFAAKVATENGMD
                     GVELHGTHGYLIQQSFSPFANQRDDKWGEELVFVKTLARRVRDAIGPGKIMGFRISAD
                     DFIDPADGGVGHERLCHIASEVVGTGLFDYLNHSEGAGGVHYARAIGSFRHKFGEYLP
                     LTRNLRDAIGGAVPLIGVGKIPTPDLAEQALQAGDCDLVGMTRAQIADPDLVSKVAAG
                     RAHRVRTCTGSNQGCFERASYPITCFQNPEVGEENRFKALDIPVVQLKKVLVVGGGPA
                     GMKAAEIAARRGHSVTLAESGDRLGGRLNLVETAGDATNLLSATAWIEEELGGLDVDI
                     RLQTTVDEAFVRQVNPDAIVLATGAYVTNDLGVATDGSVQVLSTDDAASGTFEGNRFE
                     LEGTRCLMIDFRGNYETAVVLESLGRRGSKVTLVTPAPVFGRNIGVSHADDFRRTVIP
                     QLGIRLLPNTTLAQISQGRGHLVNHVTGEEIEEFFDFIVAGTPPRPRDVLFEVLGRHA
                     PVTVVGDAVAPRDAMLAFREGDRAGRTI"
     gene            complement(5120..>5831)
                     /locus_tag="AC16QF_16330"
     CDS             complement(5120..>5831)
                     /locus_tag="AC16QF_16330"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6450179.1"
                     /translation="PYLEATPFTKQRHELTGSIYERVDDGRVKVTTTDNRVGYFDDRG
                     NHIDGEKFHADIHLCNWVGGKLIEGMDELPSSVITGDFAEKRAKAAAVRREELRKILG
                     DKMDEVSDAELLDPIYYSLFPNVSPWGSFNQIFYRFRPYGDNPEECIHECLFMWPVAE
                     GQPRPPAAKMQWLDLDDDYVDAPELGHLAKVFNQDTLNLPYVQKGLHSLDEVIFANYG
                     ETKPRHFHMLYNEWMSKE"
BASE COUNT         1040 a         1822 c         1865 g         1104 t
ORIGIN      
        1 aatacttttt cccgatcgcc gtcgcgatcc aggttcatga agcgcacgag ctggctgttg
       61 gaggggccga tcatggcctt ctgcggcgtc gcgatcagcc cgggattttc attgccccgc
      121 aacgaggtca gggcctggtc gtcctctccc ggccgctcga cctgaaagga ttcgatgtcg
      181 acgaaaaggt tatcaccggc gtcgatgttg accagttcca catcctggcg atgctggctg
      241 ggagagtcga agacaacaat ggcgcgattg accaccagct ccgcgcacgc ggagccggtg
      301 gccaatgcga ccatggagca aacgatgatc gaacagatat ggaaacgctc agttttcacg
      361 gcagcctcct atttggcgtc tggttgatgt gcgtggtcac acgggaacga cgaacagccg
      421 gacgtggccg gtcaggtttg tgagttcggt gtccggcgca gatcgggtgg tgctgatcga
      481 caggcgaaga gagttctccg gcgtgcagtc gcgaccgctg gatatgaagg tcgccgacgc
      541 gcctccttcg gccccaaggc taacctcgta actcacggcg gagcccgatg ccgtgcgcag
      601 cccggaggct tcgtcaccgc cgtctggcaa cgcggacact tcaaaggcct gcccgctggg
      661 cgcgttgaga cacagcggca gcaccgcctc acccgtctcg ctgagatccc ggctcgacag
      721 attcaggtcg ggtctccctc gcccgccgcc gggaggcgcg tccgggcgcc cgccggtacc
      781 gggtcccctg acaatccgcg caccgctccg gattcgcaac gtcacgcgcg cttctgcttt
      841 gctggtctcg ccaaacgccc catcgatccc gttcggctgg gcgccccggg cgcttccgcc
      901 cccgacgacg accagcaagg cgatgccaag gcagagcgca ttgaccgaag cagttcgcaa
      961 acgtgccaac atcgccgcta ctccggtacg accagcagaa ccagcgtgtc cccataggag
     1021 cctgagggga cggcctgcac gtcggcctct aaggcgcgca cggtgatggt ggcgttgtcg
     1081 gagccgccgc agtagctgtc ggggctgccc agggccgaga tggagttgtt ggtgatctgc
     1141 tccgtgccgc tgccagaccc tagagaagag aagagcacgt ccacaggcag gtaaacggca
     1201 tccgtggcgt gcttcagtcg aaaatcgccc ccaggggtct gattgctgga tgagacgctg
     1261 atgcggtagg cagcgttgtc cgcgaggctc tgcacgcaga aatgctcgtc ctgccgaacg
     1321 ttaccggtcc cggggacgta agtacccagc gccatgtcat ccataccgct gatacggacg
     1381 tttgcgctct gcacggtgag gatcagatcg aaatcggtcc ggtcgtactt cgtcccgctc
     1441 gttcccccca ccgcttcgag ccggaactgc ccgtgataga ccccggccac cttgttcgtc
     1501 aggttcgcct ccggaatgga gatgcgcagt tccgagttgt ccccggtccc aaggcattcc
     1561 tggaagcggc cgagatggta gtgcgtgtca aagaccccct cagtgagggt ctcgaacgcg
     1621 ctccccgcaa cgatgtcgcg gtgttcgacc acaacggtga tgcgggcact ttcaggactc
     1681 gaggcgtcgt tgttcagata aagatagaag tccgagcctg gattgcggtt gtccacaccg
     1741 gcctggtacg ggtaaacctc cgccggcggg ctcggcgggt tgggctggcg ctcattggca
     1801 gaagcaatgc agccgcgcac ggtggtggac aggcttccac cggaccaggt gccgaagtcc
     1861 acgtcgggga cgccgccaac gcttaagtag ttgtcgttac caatgtttgc ggcataggtc
     1921 atcttaggaa acccggcgga cagcaggacg caaagcccgg tgagcaggag cggaagttgc
     1981 ggccatctgt gtagaggaat ggggtgcaag tcggatcagc tcggtgagtg aaaacatgtc
     2041 ggtcagctcg gaaaccgggc gccgcgggcc tggtgcccgt gttcgggcac caggtcaccg
     2101 ccccgggtca ctgaggcgaa accagcagcg agacggtcgt ggcgtaatcg ctggccgtcg
     2161 tcgcggcgcg gatatccgca tcctccacct cgacgttgat gtacgcgttg tcactgccgc
     2221 cgcagtcgat cagggcagct cccgtgtagt tggaagagac acctccggtg ttgacgtctt
     2281 cggcgcccgt gccggcagcg ttgtcaatga gcgacacctg cacggcgatc gtgtccgacg
     2341 tggtcgggct cgccaggggt ccgtaggttg ccgtgatgtt gtatgcgtca ccgccgttga
     2401 cgtgcacgca gaacgtatcc gaagcgtcca cgccgtccgt gtcggtggct gcgtaggtgc
     2461 cgaggtcgat gtcgtccagc ccggtgatgc gggtggcatc cacgaccgtc aggctgacgt
     2521 cggtggtgcc ggtgctggtc gtgtcgtagg acccgtcggt ggccgccatg gcggagcttg
     2581 cggcgaggag gccgagggta gtcagggcaa ttgcggaggt ctttttcact gttcagattc
     2641 cattggtagt tgagtcacac gtttacgacg accttcaggt gaaggatctg ccgcgcgctt
     2701 caacagccgt tgctgagttc cgatctgcct gagctgggat cccggcatac tgcatcgaag
     2761 cttcgagccc ctggtgcagg gggcgtgcca actttccggc aatgcaattt tgctggccgc
     2821 gtcgccgcct gaaattctca tgaatttcag cttgttagcg acgagcgaag aaattttccc
     2881 gacgagcggc cgctgctcgc cgtagccgac atggatcgcg cgcgattgtc acagcgggtc
     2941 acatttcgac ccacgctgtg acgccttcgt cagtcgagcc gagaagccag gaacacgatt
     3001 tgtgcccagc gcgtcatggc ggcagagaga tcgggcacgc gggggtcaga tggtgcggcc
     3061 cgcccggtcc ccctcgcgga acgccagcat cgcatcccgc ggcgcgacgg catcgcccac
     3121 cactgtcacc ggggcatggc gaccgagaac ctcgaagagg acgtcccggg gccggggcgg
     3181 cgtgccggca acgatgaagt cgaagaactc ttcgatctcc tcacccgtca cgtgattgac
     3241 cagatggccc ctgccctggc tgatctgcgc cagcgtcgtg ttgggcagca agcggattcc
     3301 cagctgcgga atgaccgtgc gtctgaagtc gtcggcgtgg gagacgccga tgttgcggcc
     3361 gaagaccggc gccggggtca ccagggtaac cttgctgccc cggcgtccga gcgattcgag
     3421 caccacggcc gtttcgtaat tgccgcggaa atcgatcatc aaacagcgcg tcccttccag
     3481 ctcgaagcgg ttgccctcga aggtgccgga ggctgcgtca tcggtggaca gcacctgcac
     3541 ggaaccgtcg gttgcgacgc cgaggtcatt ggtcacgtag gcgccggtgg cgaggacgat
     3601 ggcgtccgga ttcacctggc ggacgaatgc ttcgtctacg gtggtctgca gcctgatatc
     3661 gacatccagg ccgccaagct cctcttcgat ccatgcggtt gcgctcaaca ggttggtagc
     3721 gtcgccggcc gtttccacca ggttgagccg tccgccaagc cggtcaccag actcggccag
     3781 ggtgaccgag tgtccgcgcc tggcggctat ctcagccgcc ttcatgcccg ccggaccacc
     3841 gcccaccacc agcaccttct tgagctggac cacggggatg tcgagggctt tgaagcggtt
     3901 ttcctcacca acctcggggt tctggaaaca ggtgattgga tacgaggcgc gctcgaagca
     3961 gccctggttg gagccggtgc aggtgcgcac ccgatgggct cggcccgcgg ccaccttgct
     4021 gaccagatcg ggatccgcaa tttgcgcgcg ggtcatgccg acgagatcgc agtcgccggc
     4081 ctggagcgcc tgctccgcca gatccggggt cggaatcttc cccacaccga tgaggggcac
     4141 cgcaccccca atcgcatcgc gcaggttgcg cgtcagcggc aggtactcgc cgaacttgtg
     4201 ccggaacgag ccgatggcgc gcgcatagtg cacgccgccg gcaccctcgg aatggttcag
     4261 gtagtcgaag aggcccgtgc ccaccacctc ggaggcgatg tggcatagcc gctcatggcc
     4321 cacgccgccg tcggccggat cgatgaagtc gtcggccgag attcgaaatc ccatgatctt
     4381 tccaggcccg atggcatccc gaacccggcg cgccagggtc ttcacgaaaa cgagctcctc
     4441 accccacttg tcgtcgcgct ggttggcaaa ggggctgaaa gactgctgga tcagatagcc
     4501 gtgggtgccg tgcagctcca cgccgtccat gccgttctcg gtagcgacct tggcggcgaa
     4561 ggcgaaagca tcgatgacct cctcgatctc cgcgtcggtc atcttgtggg tgacctcgcc
     4621 ttccagcgtc gtggttccgg agaagctcca caaaggatga aagtcatcgc gagggtccga
     4681 cttgccctga gcaccgaaat gaaacagctg ggagatgcat ttcgccccgt gccgatgcag
     4741 ccgcgtcgag atctgcgcga acttcggcgc catggtgtcc gcatcgaaaa cgtagtggta
     4801 ggggatctgg ctcgattcgt gtacgtgcat ggccgtgaag atgatcagcc cggtcccgcc
     4861 ctgggcacga cgctcctgat acgccatgta gcgttcgcca tcggagacgg gattgaagaa
     4921 ctcgaggaac gcagcgtggg cggtggagac cacccggttc ttcacctcga tcgggccaac
     4981 ctcgacaggg ctgagcagct tcatcatggc gttcctcgca gaccgatact tcgagtgtag
     5041 taccgccaca gagcaaatgt ctcgattcgt tcgacaggca tcggcccccg ctcggctcag
     5101 cgaattccgc ccgccttgct tactccttcg acatccactc gttgtagagc atgtggaagt
     5161 ggcgcggttt ggtttctccg taattggcga agatcacctc gtccagcgaa tgcagcccct
     5221 tctgcacgta gggcaggttc agggtgtcct ggttgaagac tttcgcgaga tgtccgagct
     5281 ccggcgcgtc gacatagtca tcgtccagat ccagccactg catcttcgcc gccggcggac
     5341 gcggttgtcc ttcggctacc ggccacatga acagacactc gtggatgcac tcctccgggt
     5401 tgtcgccgta aggtcggaaa cggtagaaga tctggttgaa agacccccag gggctgacgt
     5461 tggggaacag gctgtagtag atgggatcga gcagctccgc gtcgctcacc tcgtccatct
     5521 tgtcgccgag aatcttgcgc agctcttcgc gccgcacggc agccgccttc gcacgctttt
     5581 cggcaaagtc gcccgtgatt accgaagagg gcagctcgtc catcccctcg atcagcttcc
     5641 cgcccaccca gttgcacaag tgaatgtcgg cgtggaactt ctcaccatcg atgtggttgc
     5701 cacgatcatc gaagtagccc acgcggttgt cggtggtggt gaccttgacg cgcccgtcat
     5761 cgactcgttc gtaaatgctg ccggtgagct cgtgacgctg cttcgtgaac ggcgtcgcct
     5821 ccagataggg g
//
LOCUS       DBNHLM010000356         3015 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_533339, whole genome shotgun sequence.
ACCESSION   DBNHLM010000356 DBNHLM010000000
VERSION     DBNHLM010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_533339"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="AC16QF_16335"
     CDS             <1..319
                     /locus_tag="AC16QF_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450180.1"
                     /translation="TWEPAADLPRAHPRKVGLRTLVRRIYRRPTPLPRRPPNERNDNW
                     LMQGLEYGPVVRSMGPYVVSGGWWASTVHREYYFVETRRGDLLWTYYDRKRRRWFLHG
                     KVE"
     gene            complement(668..1092)
                     /gene="tnpA"
                     /locus_tag="AC16QF_16340"
                     /pseudo
     CDS             complement(668..1092)
                     /gene="tnpA"
                     /locus_tag="AC16QF_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012164419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1318..2157
                     /locus_tag="AC16QF_16345"
     CDS             1318..2157
                     /locus_tag="AC16QF_16345"
                     /inference="COORDINATES: protein motif:HMM:NF025080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HIR6450181.1"
                     /translation="MTDRTRQSVLFPSLLDRPVHVAFDHPAMSSDGGVLLLKAVDRRI
                     GLSDELAACLRDGRDQSKVQHTLQDLLCQRVFGLACGYGDTNDVARIGADPVFKLLLD
                     RDPFAGQDLASQPTLSRFENSVGSTELYAMGMAMSKKVVSYHKKRLGRRRVKRVTIDM
                     DPTDTATYGGQQLTFFNAHYGHYCYLPMLGFLTFNDEPEQYLFAAVLRSGTAPASQGA
                     IGLLRRIIAQVRKAFPKAKLRVRLDGGFACPAVSSSFSTRRSSSMSSASGKTVFSSAA
                     PGG"
     gene            2115..2696
                     /locus_tag="AC16QF_16350"
     CDS             2115..2696
                     /locus_tag="AC16QF_16350"
                     /inference="COORDINATES: protein motif:HMM:NF025080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR6450182.1"
                     /translation="MGKNSVLKRRAGRLMGTARRLSREKNHSVALFGQTRYAAKSWKN
                     TKRRVVYKAEVTRLAGREPRDNPRFVVTNMRYKPETVYQIYRMRGESENRIKELLIGL
                     ELDRMSCSSFKANQLRILLTAAAYVLIQTFRCRLSRTRLGRRQVDSIRLMILKIGAKI
                     GRSVRRYVVSMAENHPWRDDWIMAARAWGAIVT"
BASE COUNT          577 a          979 c          895 g          564 t
ORIGIN      
        1 cacatgggag ccggccgccg acctcccacg cgcacaccca cgcaaggtag ggctgcgcac
       61 gctggtccgg cgcatctaca gacgccccac gcccctgccg cggcgtccgc cgaacgaacg
      121 caacgacaac tggctcatgc agggtctgga gtacggcccg gtcgtacgct cgatggggcc
      181 atatgtggtc tccggcggct ggtgggcttc caccgtgcat cgcgagtact acttcgtcga
      241 gactcgtcgc ggcgatctgc tctggacgta ctatgaccgc aagcgccgcc gctggttcct
      301 gcacggcaaa gtagaatgag ctcatcgggc gcaaacaaga ccggtcgcgg aaaggatcag
      361 ccgccggagg cggcgtcgtg agaggccagc gcttctagca gcgaggcgtg aacgcgagcc
      421 ggcgagcgtg cagggttcga aggctacagt acgcccgcca gcgaatagcg agcacggccc
      481 gagatgatgg acgttcgggc gtcggagcag agtgcttctt gaataatccg gagtaacgat
      541 ccctcagctc agctcagctc agctcagctc agctcagcaa agccgggggc tttcattgtg
      601 agccgctcaa agcggcttgg acagggccgc tgcgcggccc cttgcgccac ctgaaggtgg
      661 cttcccctta cagcaatccc agctggtctt gcctcttatc ctcggcttcc tggtgccgga
      721 tgtactcccg gattacctct tcgtcacgac ccaccgtcga gacgaagtac cctcgtgccc
      781 agaagtgctc tccaacgaag ttgcgtttcc gttctccata gttgcgcgca atgtggatcg
      841 cgctcttccc tttgatgtac cccacgacct gagagaccga gggcttcgga ggaatcgaga
      901 tcatcacgtg gacgtggtcg gccatcagat gcccctcctc cacccagctc tccttttgct
      961 cggccagccg acggaaaacc ttcccgaggt cgcgccgcag cgcaccgtag atctgtttcc
     1021 gacggtactt cgggatgaac acaacgtgat acactcccat ttcgtatgct gtagcctctt
     1081 gtagttcgac acttggctcc ctctcgtgat gctttgagcg gctcacggga gggagtctcc
     1141 tcgtcagatt tccggaactg tcaaactttg gctgcctccc cggcagagcc ggggggactc
     1201 ccgattggtc tagcctgaat aattcacgag gtaagcacag acagaaaaga gaaaagcctc
     1261 cgccgctgct agaatccgcg taccagcgag aattccaaac agcaggaagg ctttctcatg
     1321 acggatcgta cgcgacagtc tgttcttttt ccatcgctgc tcgacaggcc tgtgcacgta
     1381 gctttcgacc atccggcgat gagctccgac ggtggtgtct tgctgctcaa ggccgtcgac
     1441 cgaaggatcg gcctgagcga tgagctggct gcctgcctgc gcgacggccg cgatcagagc
     1501 aaggtgcagc acacgctcca ggatctgctc tgccagcgcg tgttcgggct tgcctgtggc
     1561 tacggcgata ccaacgatgt cgcccgcatc ggtgccgatc ccgttttcaa gctgctgttg
     1621 gatcgcgacc catttgccgg ccaggatctc gcctcgcagc ctacgctgtc gcgcttcgag
     1681 aactcggtcg gctcgaccga gctttacgct atgggcatgg cgatgagcaa gaaggtcgtc
     1741 tcctaccaca agaagcgcct tggccgccgg cgcgtaaagc gggtgaccat cgacatggac
     1801 cccaccgata cggccactta tggaggccaa cagctgacct tcttcaacgc ccactacggc
     1861 cactactgct acctgccgat gctcggcttt ctcaccttca acgatgagcc cgagcagtat
     1921 ctgttcgcgg ccgtactgcg ttcgggcact gcgccggcat cgcaaggggc cattggtctg
     1981 ctgcgacgga tcatcgcaca agtgcgcaag gcctttccca aggccaagct acgggtgcgg
     2041 ctcgacggcg gctttgcctg ccccgcagtc tcttcgagtt tctcgactcg gagaagctcg
     2101 agtatgtcgt cggcatcggg aaaaacagtg ttctcaagcg ccgcgccggg cggctgatgg
     2161 gtacggccag gcggctttcg cgcgagaaga accacagcgt cgcgctgttc ggccagacgc
     2221 gctatgcggc caagtcgtgg aagaatacca agcggcgggt ggtctacaag gccgaggtca
     2281 cgcggcttgc gggacgagag ccgcgcgaca acccgcgttt tgtcgtaacc aacatgcgct
     2341 acaaaccgga gacggtctat cagatctacc gcatgcgtgg cgagagcgag aacaggatca
     2401 aggagctgct catcggcctg gagctcgacc gaatgagctg cagcagcttc aaggccaacc
     2461 aactacgcat cctgctcacc gctgccgcct acgtgctgat ccagaccttt cgttgtcggc
     2521 tctcgcgcac acgccttggc cgccggcagg tcgattcgat tcgactcatg atcttgaaga
     2581 tcggcgccaa gatcggtcga tccgtgcgcc gctacgtggt cagcatggcc gagaaccacc
     2641 cgtggcgaga cgactggatc atggccgcca gagcctgggg cgcgatcgtc acctaaccgc
     2701 gcccatctca ctcacgaacg aatagcatcg aggttgcaca cggcaccggt gcgcgcacat
     2761 tgcgaacgct cgagggccga ttggccgccg caacgctgac agaccagctc gcaccctgat
     2821 cttggcctcc ctgcgctggc ggatcgccga ccacaaggcg gcgagtacgc tcatctcgac
     2881 tcgacgctcc cagtcatgaa taaaggccgc tagcgtgcgc gcgcgtgaag actcactcaa
     2941 gcagccacgg gccgccggac cgaaatcccc gccgttggct gagctcgcgt gcaggacttg
     3001 ttcgacggct ccgcc
//
LOCUS       DBNHLM010000357         8157 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_831663, whole genome shotgun sequence.
ACCESSION   DBNHLM010000357 DBNHLM010000000
VERSION     DBNHLM010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8157)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_831663"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="AC16QF_16355"
     CDS             complement(<1..475)
                     /locus_tag="AC16QF_16355"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6450183.1"
                     /translation="MERTVKISQGRHAIEDIMYGYAEGIDTGNLETVAPLFAHGVMVM
                     PDGSELNGPEEVLRTFSDVIIFYDDEENVVPYQRGACSPRTKHVVTNSIFEFDNAVRN
                     ADVRSYFTVYQSLGGANEIIVGGRYIDQYELMIQGWVLVRREVYLENVGDLSRHLN"
     gene            607..1707
                     /locus_tag="AC16QF_16360"
     CDS             607..1707
                     /locus_tag="AC16QF_16360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6450184.1"
                     /translation="MAKRLIVMAGLLALVFGSLFGWKAFERHKTAEYLANARPPAVVV
                     SSTRVEEDRWRQQVESVGSLSAIQGVDVSSEVPGTVADISFQSGQQISAGELLVQLDA
                     TAERAELRSLEAQLVLARLDYERALGLLERTMLSQAQLDRAKSVMDSLQAQVEEQAAF
                     IAKKAIRAPFAGELGIRQVNLGEYVSPGTPIVTLQALDPIYANFALPERFLRELAVAQ
                     PLRVVVAAYPEDEFLGTVTAISPKVEETTRNVVVQATIANADRRLRPGMFARITVQTG
                     GDHRVLTVPRTAITFNPYGESVFLINEQDGALLVDRRQVITGEIRNERVEVVSGLLAD
                     DLVVATGQLKLRAGQRVKIDNSIDLSRPVTGP"
     gene            1704..4790
                     /locus_tag="AC16QF_16365"
     CDS             1704..4790
                     /locus_tag="AC16QF_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6450185.1"
                     /translation="MNFTDTFVRRPVLASVISLLVLLVGIRAAGLLELREYPESNRAT
                     ITVTTAYPGADADLVQSFITVPLQRAISEAEGIDYLTSDSRQGVSIIEAFMELNYDPN
                     AAIAEIQAKVASQRNLLPQESMDPVIDSRTSLGWALMYLAFTSQEMSPAQVTDYLHRS
                     VIPRLQAIPGVAKAQINGNPTLAMRIWLNPHRMAALGVTATDVRAALQRNNFQAGVGE
                     TKGDYVRVTLSSTTGITAREDFARLIIRRDGETLIRLRDVAEVNLDSKNYDQTSWYNG
                     QPAILLALETSPGANPLTVSQAVRVLLPDIESQLPSNMEMQLAHDGSQYIDDSINEVL
                     RTLLEAMLIVLLVIFLSLGSWHAAVVPAVSVPLSLVGACALMLAFGFSLNLLTLLAML
                     LAIGLVVDDAIVVVENVHRHIAMNKTPFQAAIDGARELGLPILSMTTTLLAVYAPIAF
                     MGGLVGILFTEFAFSLAGAVLISGLVALTFSPMLSSKVLTSGEEPGRFESAVERFFAR
                     LAERYRARLSRALDYQPVTYLFAIVVMGSIYFMFTTSNTELAPTEDREVLNVQITAPE
                     TATLEYAVAHVRPAVKMFETFPEQRRTFLMIGGGGTPATSFGGFRFTPVAERERSQME
                     MQPILQRMLNEIAGVQIAVFSFPSLPGAARGTPLQFVIASDQDYRQLDTLADDVVQAA
                     YRSGNFLFLNKSVEFALPQTQIVIDRDRAGDLGIEMNDIGVALATLLGGNYVNRFSLA
                     GRSYEVIPQVERRFRASHEMLNDNYLRAASGELVPLSSFISFEHSIEPTRRSQFQQLN
                     AVTINGVMAPGVSLGDAITFMERTAAELFPREFSYDYVGESRQFKQQGSALVVTFFLS
                     IIVIYLVLAAQFESWRDPLIILISVPMSIAGALAFVMLGFASINLYTQVGLITLIGLI
                     AKNGILIVDFANRQQLVAGLSKRAAVEQAAAVRLRPILMTTVAMLMAMVPLLIATGPG
                     AVSRFQIGLVIATGLGIGTLFTLFVVPSVYLLLARDRTDPAQRDAATPRPTPGLEP"
     gene            4787..5374
                     /locus_tag="AC16QF_16370"
     CDS             4787..5374
                     /locus_tag="AC16QF_16370"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR6450186.1"
                     /translation="MSSEAGLRHLPNLISSLRLLCAPVLIWLTVQGLSDVFAWILIAA
                     WLSDLADGWLARSFGWVSRIGAMLDSVADIALTLATLYGIWVFHNDVFAVHGWLIWLV
                     LGLWVTVNSIALVRYGRPASFHARLAQVGMALFGVFVLTLFFYDFVGWIYYLCGAVCL
                     AAGVENLAMIYLLDDWTPNVRGGLMAALRRRQGDR"
     gene            complement(5407..6189)
                     /locus_tag="AC16QF_16375"
     CDS             complement(5407..6189)
                     /locus_tag="AC16QF_16375"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6450187.1"
                     /translation="MNRLDGRVAVVTGGSRGIGAAIVRAFVDEGAMVAFNGRSAESGA
                     ALMEALGEPERVWFCQGSVTVTADLEKLIDGAVDRFGKLDILVNNAGGSNAMQPVAEL
                     DEAAWESDLRFNLTSTFLGTKLALKHMLPAGYGTVINISSVEGKMGVPVMAAYTACKH
                     GVNGFTKAVAAEVGRAGITVNAICPGLILTDAVTGGGPALAESMGMTFDEMVENVFKA
                     KTLTGELNTVEQIGETAVFLASPAGRGITGAQISVDGGMTPY"
     gene            complement(6212..7252)
                     /locus_tag="AC16QF_16380"
     CDS             complement(6212..7252)
                     /locus_tag="AC16QF_16380"
                     /inference="COORDINATES: protein motif:HMM:NF014954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6450188.1"
                     /translation="MKDYPVAPEQLTAEWLGTALGFPVSDIEVSSLGEGVGIMALVRR
                     VRFRAADGKPSSVIVKFPSPSADNRAVAHTYDMYGREVNFYRNIAPLISLPTPACYLA
                     EFDPADDDFVLVLEDVGHLRIGDQVAGCSLDEARAVLSAIAKFHADGWQPSPDRFGQV
                     ISHNNPAQRDGMIGGWQVGWPVVKAQFPDLIPSAALEAGDRMPDAVGRLLEEMCREPV
                     CLIHTDVRLDNVFFGADEIMLVDWQAVCTSAPEQDVAYFVTQSVAPEVRAQDDLVKFY
                     HGELVSHGIDYGLERCRERYRVSALYLLCFAVVIAGTLDLANERGAQLGRTLLRNAFE
                     ALDEMDAFALLS"
     gene            7251..>8157
                     /locus_tag="AC16QF_16385"
     CDS             7251..>8157
                     /locus_tag="AC16QF_16385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6450189.1"
                     /translation="MTLHSTHWTPVIHGGILPAMPMITDTSPIVFRDDLPASIDVIVI
                     GAGVAGISTALFLRERGVSVLVCEKGRVAGEQSSRNWGWIRQQRRDAAELPIMMESIN
                     LWERLASEVDEDIGFARHGVLRLAETQAELDGLAEWLPVAEQHQLETRLLNASEVDEL
                     VSGRPRQWRGGLYTPSDGRAEPFKAVPALARLLQHRGGLVRESCAVRWIDREGGRVAG
                     VITEEGLVRTQAVVCAAGAWSALVVRHLGVRLPQLTVRATVARTARAPDLFAGNANAG
                     DIAFRRRNDGGYTVAAAGENEHFVSA"
BASE COUNT         1383 a         2760 c         2583 g         1431 t
ORIGIN      
        1 cgttgaggtg gcgggacaga tctccgacgt tttccaggta cacctcgcgt ctgacgagca
       61 cccagccctg gatcatcagc tcgtactgat cgatgtagcg cccgccgacg atgatctcgt
      121 tggccccgcc cagagactga tagacggtga agtagctgcg cacgtcggcg ttgcgaaccg
      181 cgttgtcgaa ctcgaagatg ctgttggtga ccacgtgctt ggttcgcggc gagcaggcgc
      241 cgcgctgata cggaacaacg ttctcctcgt catcgtagaa gatgatgacg tcggaaaacg
      301 tccgcagcac ctcttccgga ccgttcagct cgctgccgtc cggcatgacc atgacgccat
      361 gggcaaacag aggcgccacg gtttctagat ttcccgtgtc gatgccctcg gcgtagccgt
      421 acatgatgtc ctcgatggcg tgccgcccct gagaaatctt gaccgtgcgt tccatctcac
      481 cctccgtgcc aggcgacatt atgcccattc acccgccatg gagacagcca cgggccgcct
      541 cggcgctata atgcagcgtc ttccgggggg atccagatcg atgtgcagag caaggatcga
      601 ggcgccatgg ccaagcgact gatcgtgatg gccgggctgc tggcgctggt cttcggcagc
      661 ctgttcggct ggaaggcgtt cgagcgccac aaaacggccg agtatctggc aaacgcccgg
      721 ccgccggcgg tggtcgtctc gtcaacgagg gtggaagagg accgctggcg gcagcaggtg
      781 gaatccgtcg gctcgttgag cgccatccag ggcgtggacg tcagctccga ggtgcccggc
      841 accgtcgcgg acatcagttt tcaatccggg cagcagatca gcgcaggcga gctgctcgtc
      901 cagctcgacg ccaccgccga acgggccgag ctgcgctcgc tggaagccca gctggtgctg
      961 gcgcgactgg actacgagcg cgctttaggt ctccttgagc gcacgatgtt gtcacaggcg
     1021 cagctcgatc gggccaagag cgtgatggac agcctgcagg cgcaggttga ggagcaggcc
     1081 gccttcattg ccaagaaagc catacgggca cccttcgccg gcgagctcgg tatccggcaa
     1141 gtcaaccttg gcgagtacgt ctccccgggc acgcccatcg tcactctgca ggcgcttgat
     1201 cccatctacg cgaacttcgc gctcccggaa cggttcctgc gcgagctcgc cgtggcccag
     1261 ccgctgcgcg tggtcgtggc cgcctacccg gaggatgagt tcctcggcac ggtgaccgcc
     1321 atcagcccca aggtcgagga gaccacgcgc aacgtcgtgg tccaggctac cattgccaac
     1381 gccgatcgca ggctccgtcc aggaatgttc gcgcgcatca ccgtgcagac cggcggggac
     1441 caccgggtgc tgacggttcc gcgaacggcg atcaccttca atccctacgg cgagtccgtc
     1501 ttcctcatca acgagcagga cggcgctctg ctggtcgacc gccgccaggt catcaccgga
     1561 gagatccgca acgagcgcgt ggaggtcgtt tccggcctgc tggccgacga tctcgtggtg
     1621 gccaccgggc agctgaagct gagagccgga caacgcgtga agatcgacaa cagcatcgat
     1681 ctttccaggc cggtgacggg cccttgaact tcaccgatac gttcgtccgg cgtccggtgc
     1741 tggccagcgt gatcagcctg ctcgtgctgc tggtcggcat ccgggccgcc gggctgctcg
     1801 agctgcggga gtatccggaa agcaaccgcg ccacgatcac ggtcaccacc gcctaccccg
     1861 gcgcggacgc ggacctggtg cagagcttca tcaccgtgcc cctgcagcgc gccatctcag
     1921 aagccgaggg gatcgactac ctgacatccg attcccgtca gggggtttcg atcatcgaag
     1981 ccttcatgga gctcaactac gacccgaacg cagccatcgc cgagatccag gccaaggtgg
     2041 ccagccagcg caacctcctg ccccaggaat ccatggaccc ggtgatcgat tcgcgcacga
     2101 gcctgggttg ggcgctcatg tacctcgcct tcaccagcca ggagatgtcc cccgctcagg
     2161 tgaccgacta cctgcaccgc agcgtcatcc cgcggctgca ggcaatcccc ggcgtcgcca
     2221 aggcacagat caacggaaac ccgacccttg ccatgcgcat ctggctgaat ccgcaccgca
     2281 tggcagccct gggcgtcacg gcgaccgacg tgcgggcggc gctgcaacgc aacaacttcc
     2341 aggccggcgt cggcgagacc aagggcgact acgttcgcgt caccctgagc tccaccaccg
     2401 gcatcaccgc cagggaagat ttcgccaggc tgatcatccg cagggacggc gagacgctga
     2461 tccggctgcg cgacgtcgcc gaggtgaatc tggattcgaa gaactacgac cagaccagct
     2521 ggtacaacgg ccaaccggcc atcctcctgg ccctggagac cagtccaggc gccaatcctc
     2581 tgaccgtttc ccaggccgtc cgcgtcctgc tgccggacat cgaaagccag ctgccatcga
     2641 acatggagat gcagctcgcc cacgacggca gccagtacat cgacgactcc atcaacgagg
     2701 tgctgcgaac cctgctggaa gccatgctca tcgtgctgct ggtgatcttc ctttccctcg
     2761 gctcctggca tgcggccgtg gtacccgccg tctccgtgcc gctctccctg gtcggcgcct
     2821 gcgcgctcat gctggccttc ggcttctcgc tgaacctgct caccctgctg gccatgctgc
     2881 tcgccatcgg gctggtggtg gacgacgcca tcgtggtggt ggagaacgtt caccgccaca
     2941 tcgccatgaa caagacgccg tttcaggcgg ccatagacgg ggcccgggag ctgggactgc
     3001 ccattctctc catgacgacc accctgctcg ccgtctacgc ccccatcgcc ttcatgggcg
     3061 ggctggtggg catcctcttc acggagttcg cgttctccct ggccggagcc gtgctcatct
     3121 cggggctggt ggctttgacg ttctcgccca tgctctcctc caaggtgctt accagcggcg
     3181 aggagccggg gcgattcgaa agcgccgtag agcgtttctt cgcccgactc gccgagcgct
     3241 atcgggcgag gctctcgaga gccctcgact accagcccgt cacgtacctc ttcgccatcg
     3301 tcgtcatggg cagcatctac ttcatgttca ccaccagcaa caccgagctt gcgcccaccg
     3361 aagaccggga ggtgctgaac gttcagatca ccgcgccgga aaccgccacg ctggaatacg
     3421 ccgtggcaca cgtccggcct gcagtgaaga tgttcgagac gtttccagag cagcggcgaa
     3481 ccttcctgat gatcggcggc ggcggcacgc cggccaccag cttcggcggg tttcgcttca
     3541 cgcccgtcgc ggagcgcgag cggtcccaga tggagatgca gcccatcctg cagaggatgc
     3601 tcaacgagat cgccggcgtg cagatcgccg tgttctcgtt cccctccctg ccgggcgctg
     3661 ccagaggtac gccgctgcag ttcgtgatcg cgtcggatca ggactaccgg cagctggaca
     3721 ccctggccga cgacgtggtg caggccgctt atcggagcgg caacttcctg tttctcaaca
     3781 aatcggtgga gttcgcgctg ccccagaccc agatcgtgat cgatcgggac cgggcaggtg
     3841 atctcggtat cgagatgaac gacatcggcg tcgcgctcgc gacgctgctg ggcggcaact
     3901 acgtcaaccg gttcagcctc gcaggccgga gctacgaggt gatcccccag gtcgagcgcc
     3961 gcttccgcgc cagccacgag atgctgaacg acaactacct gcgagcagcg tccggcgagc
     4021 tcgttcccct gtcgagcttc atcagcttcg agcactccat cgagcccacc cgtcgcagcc
     4081 agtttcagca gctgaacgcc gtgaccatca acggcgtcat ggcgccgggg gtatccctcg
     4141 gggatgccat caccttcatg gagcgcaccg ccgcagagct gtttccccgc gagttctcct
     4201 acgactacgt gggcgagtcc cgtcagttca agcagcaggg cagcgctctg gtggtgacgt
     4261 tctttctgtc gatcatcgtc atctatctgg tgctggcggc ccagttcgag agctggcggg
     4321 atccgctgat catcctcatc tcggtgccca tgtccatcgc cggcgcgctg gcgttcgtca
     4381 tgctcggatt cgcctccatc aacctctaca cccaggtggg gctgatcacg ctcatcggcc
     4441 tgatcgcgaa gaacggcata ctcatcgtcg acttcgccaa ccgccagcag ctcgtcgccg
     4501 ggctcagcaa gcgggcagcg gtggagcagg ccgcggcggt ccggttgcgg ccgatcctga
     4561 tgaccacggt agccatgctc atggccatgg tgccgctgct catcgccacc gggccagggg
     4621 cggtgagccg cttccagatc ggcctcgtga tcgccacggg gctcggcatc ggcaccttgt
     4681 tcacgctgtt cgtcgtgccg agcgtctatc ttttgctggc aagggaccga accgatcctg
     4741 cgcagcgcga tgcagcaacg ccccgcccga cgccggggct cgagccatga gttcagaggc
     4801 aggcctgcgt cacctgccca acctgatctc gagcctgcgt cttctctgcg cgcccgtgct
     4861 gatctggctc acggtgcagg ggctgtcgga cgtattcgcc tggatcctga tcgcagcctg
     4921 gctcagcgat cttgcagacg ggtggctggc ccgctcgttc ggctgggtca gcaggattgg
     4981 cgccatgctg gattccgtcg ccgacatcgc attgacgctg gccacgcttt acggcatctg
     5041 ggtctttcac aacgacgtct tcgccgtgca cggctggctg atctggctgg ttctcggcct
     5101 gtgggtgaca gtcaattcca tcgcgctcgt ccggtacgga cgaccggcca gcttccacgc
     5161 gaggctcgca caggtcggca tggccctctt cggggttttc gttttgacgc tcttcttcta
     5221 cgacttcgtc ggctggatct actacctgtg cggcgccgtc tgcctggctg cgggcgtgga
     5281 aaacctcgcc atgatctacc tgctcgacga ctggacgccg aacgtgcgag gcggcctcat
     5341 ggccgcgctg cggcgccgcc agggcgaccg ctgattgcag gcaccggcaa ccgcccgatg
     5401 ccgctgctag tagggcgtca tgcccccgtc gacggagatc tgggcacccg tgatcccgcg
     5461 gccggccggt gaggccagaa aaacggcggt ctcgccgatc tgctccaccg tgttcagctc
     5521 acccgtcagc gttttcgcct tgaagacgtt ttccaccatc tcatcgaacg tcatgcccat
     5581 ggattccgcc agagcagggc cgccgccggt taccgcgtcg gtgaggatca ggcccgggca
     5641 gatggcgttc acggtgatgc ccgcacgccc cacctccgct gccacggcct tggtgaagcc
     5701 attcacgccg tgcttgcagg ccgtgtaggc ggccatgacc ggcaccccca tcttgccctc
     5761 cacggaggag atgttgatga ccgtgccgta cccggccgga agcatgtgct tgagcgccag
     5821 cttggtgccc aggaacgtcg aggtgaggtt gaatctcaga tcgctctccc aggcggcctc
     5881 gtccagctcc gcaaccggct gcatggcgtt gctgcccccg gcattgttca ccaggatgtc
     5941 cagcttgccg aagcgatcga ccgcgccgtc gatgagcttc tcgagatccg ccgtgacggt
     6001 cacggagccc tgacagaacc agacccgctc gggctcacca agggcttcca tcagcgcagc
     6061 gccagactca gcggagcggc cgttgaacgc caccatggcg ccctcgtcga cgaatgcccg
     6121 aacgatcgcg gcgccgatgc cgcggctgcc gccggtgacc acggccaccc ggccgtccaa
     6181 tcgattcatg atccctcccg aatgccggct ctcagctcag aagcgcgaag gcgtccatct
     6241 cgtccagcgc ttcaaaagca ttgcgcagca gcgttcggcc aagctgggcg cccctttcgt
     6301 tggcgagatc cagggtcccg gcgatgacca ccgcaaagca gagcaggtaa agggctgaaa
     6361 cccggtaacg ctcccggcag cgctcgaggc cgtagtcgat gccgtggctg acgagctcgc
     6421 cgtgatagaa cttcaccaga tcgtcctggg cccgcacctc gggcgcaacg ctttgggtga
     6481 caaagtaggc aacgtcctgc tcgggggcag aggtgcacac agcctgccag tccaccagca
     6541 tgatctcgtc agcgccgaag aagacgttgt ccagacgcac gtcggtgtgg atgaggcaaa
     6601 cgggttcccg gcacatttcc tccagcagcc gccccacggc gtcgggcatc ctgtcccccg
     6661 cctcgagggc ggccgacggg atcagatccg gaaactgggc cttgaccacg ggccaaccca
     6721 cctgccagcc gccgatcatc ccgtcgcgct gggcggggtt gttgtgggaa atgacctggc
     6781 cgaaccggtc cggcgagggc tgccacccgt cggcatgaaa cttcgctatc gcggaaagca
     6841 cggcccgcgc ctcgtcgagg ctgcaaccgg ccacctgatc gccgatgcgc aggtggccca
     6901 cgtcctcgag cacgagcacg aagtcgtcgt cggcagggtc gaactcggcg aggtaacaag
     6961 ccggcgtggg gagcgagatc aaaggcgcga tgttgcggta gaagttcact tcgcgcccgt
     7021 acatgtcgta ggtatgggcg accgcgcggt tgtctgccga cggcgaggga aacttgacga
     7081 tgaccgagga aggcttgcca tccgcagccc taaacctgac ccggcggacg agcgccatga
     7141 tgcccacccc ttctccgagg gaagagactt cgatatcgct caccgggaat ccaagggccg
     7201 tgccgagcca ctcggcggtc agttgctctg gagccacggg gtagtctttc atgactctgc
     7261 attctaccca ttggacaccg gtaatccacg gcggaatact gcccgccatg ccgatgatca
     7321 ccgacacgtc ccccatcgtc tttcgcgacg atctgccagc aagcatcgac gtaatcgtca
     7381 tcggcgctgg cgtggccggc atcagcacgg cgctctttct gcgcgagcgc ggcgtcagcg
     7441 tgctcgtctg cgagaaggga cgcgtggcgg gcgagcagtc gagccgcaac tggggctgga
     7501 tacgccagca gcgacgggac gccgccgagc tgcccatcat gatggaaagc atcaacctgt
     7561 gggaacgcct cgcctcggag gtcgacgagg acatcggctt cgcgcgccac ggggttctgc
     7621 gactcgctga aacacaggcg gagctggacg gtctggccga gtggctgccc gtggccgagc
     7681 agcatcagct ggagacgcgt ctgctgaacg catccgaggt ggatgagctc gtgagcggtc
     7741 ggccccgaca gtggcggggc ggtttgtaca cgccgagcga cgggcgcgcg gagcctttca
     7801 aagccgtgcc ggcgctggcg cgcctgcttc agcatcgggg cgggctcgtg cgcgagtcct
     7861 gcgcggtgcg ctggatcgat cgggagggcg gccgcgtcgc cggggtgatc accgaggaag
     7921 gcctggttcg cacccaggcc gtggtgtgcg cggccggcgc ctggtcggct ctggtcgtgc
     7981 ggcatctggg cgttcgcttg ccgcaactca ccgtgcgcgc cacggtcgcg cgcacggcca
     8041 gggccccgga cctcttcgcg ggcaacgcca atgcggggga catcgccttt cggcgccgaa
     8101 atgacggggg ctacacggtg gcggcggccg gcgagaacga gcatttcgtg agcgccg
//
LOCUS       DBNHLM010000358        15875 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_713083, whole genome shotgun sequence.
ACCESSION   DBNHLM010000358 DBNHLM010000000
VERSION     DBNHLM010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 15875)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15875)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15875
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_713083"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="AC16QF_16390"
     CDS             <1..304
                     /locus_tag="AC16QF_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HIR6450190.1"
                     /translation="PKETLKAEGFTDEQIDEGFELFEPVGCDSCNNGYKGRVGIYEVV
                     KITPEMSRIIMEDGNSIKLAETASKLGFKNLRQSGLLKVIQGVTSLAEANRMTTGH"
     gene            308..1522
                     /locus_tag="AC16QF_16395"
     CDS             308..1522
                     /locus_tag="AC16QF_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR6450191.1"
                     /translation="MAESAIFLWEGIDKGGRKTKGEISSSTPAIAKAELRRQGINATK
                     VKKKSKAFSLGGGGSVKPVDIALFTRQMSTMMRAGVPLVQAFEIVAGGVDKPKVATLI
                     RALRDDVAGGSSFTAALRKHPLHFDDLFCNLVGAGEQSGALETMLDRIATYKEKTESL
                     KAKVRKAMTYPAAVLVVAFIVSGILLIKVVPQFEEVFAGFGAELPAFTQFVINISEFT
                     QQWWFLIVVAIGGTIATFMAARKRSKKLREAIDRIALKAPIFGNIIEKSAVARYARTL
                     ATTFAAGVPLVEALNSVAGSTGNSVYTKAVYQIRDDVSTGQQLNFSMRSTGVFPNMVV
                     QMVAIGEEAGALDEMLDKSASYYEEQVDNAVDNLTALMEPMIMAVLGVLVGGLIIAMY
                     LPIFQLGRVVGG"
     gene            1572..2426
                     /locus_tag="AC16QF_16400"
     CDS             1572..2426
                     /locus_tag="AC16QF_16400"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR6450192.1"
                     /translation="MGETLLAYGWAGLTFAVWVGLSVGSFLNVVIYRLPIMLSRQWEA
                     QARELLEEEPAETGQERFNLMVPRSRCPACQQPISAWQNVPVFSWLFLKGRCRSCGVR
                     ISIRYPMVEVLTGAATITVLALFGYTWLGLAAAVYTWAMIALTFIDYDTQLLPDQITL
                     PLLWLGLITCHFGGVTDLGSGVLGAVIGYLFLWTTFWAFKLITGKEGMGYGDFKLFAA
                     IGAWLGWQVLPGTILIAATLGLAYALAITLAGKREAAQPIPFGPFLAVAGWVSLMARD
                     NVLTLFAA"
     gene            2456..3055
                     /gene="coaE"
                     /locus_tag="AC16QF_16405"
     CDS             2456..3055
                     /gene="coaE"
                     /locus_tag="AC16QF_16405"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003302803.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR6450193.1"
                     /translation="MSRLVIGITGGIGSGKTAVSDRFKALGVTVVDADIASRVIVEPG
                     RPALVAIAEHFGCDVIQADGSLDRAELRRRVFADPDERRWLEQLTHPLINEHIREELE
                     QAESPYAVLVSPLLAETGQSRFCHRILVVDVPEEVQVRRTMARDANDEAQVRKIIEAQ
                     ASREQRLALADDVIVNDSSLEVLDEEVARLHQVYTEMAQ"
     gene            3052..3264
                     /gene="yacG"
                     /locus_tag="AC16QF_16410"
     CDS             3052..3264
                     /gene="yacG"
                     /locus_tag="AC16QF_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HIR6450194.1"
                     /translation="MTASEPDRTARIVTCPTCGGRVKWTSGNEFRPFCSERCKLMDLG
                     AWASEQHRIPGNEVDDDLLSGDLDPD"
     gene            complement(3292..4512)
                     /gene="argJ"
                     /locus_tag="AC16QF_16415"
     CDS             complement(3292..4512)
                     /gene="argJ"
                     /locus_tag="AC16QF_16415"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019471521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR6450195.1"
                     /translation="MAVNVPPLQELLVVPGVSLGTAGAAIKRQGRDDIAVLLLHPQTR
                     AAGVFTRSSFRAAPVTLADRRLSGGRARALVINSGNANAATGDPGLADAVAVCDAVAA
                     ALDVPGDSVLPFSTGVIGERLPVSRMLPAVVSACGSLAPDGWEAAARAIMTTDTAPKA
                     VSRRVRVAGEEVVITGMAKGAGMIKPDMATMLGFVCCDAAVADGCLPAMVREVAGRSF
                     NRITVDGDTSTNDAFVVCATGCAENAPVTDVGSEGYAELRDGLAAVATELAQSIVRDG
                     EGATRFVTVRVSGGESAAECLKVAYTVAESPLVKTAIFAGDPNWGRFCMAIGRAGIAD
                     LDTGRVAVHLDDVPIALGGLVAPQYEEAAAARVMARQEFEVHVDLGRGSAEETVWTTD
                     LSYEYVRINAEYRT"
     gene            complement(4526..7249)
                     /gene="secA"
                     /locus_tag="AC16QF_16420"
     CDS             complement(4526..7249)
                     /gene="secA"
                     /locus_tag="AC16QF_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR6450196.1"
                     /translation="MLKKIFGTKNARELKRMGRIVARINALEADLLEISDDELKATTG
                     RLKERLEQGASLEDLLPEAFAAVRESARRAKDMRHFDVQLIGGITLHEGRIAEMRTGE
                     GKTLVATLAAYLNALAGRGVHVVTVNDYLARRDAEWMGPIYETLGLTVGVIQSGQAPQ
                     EKREAYQSDVTYGTNNEFGFDYLRDNMAFSLADKFQRDLGYAIVDEVDSILIDEARTP
                     LIISGPAEQSTELYQKIDRIAPKLKQQPPVEDPEAEPTGDFVVDEKNRQVELTEVGHE
                     KVEAELMRLGLLEEGDSLYAAANLNLLHHVHAALKAHALFKRDVDYMVANGEVVIVDE
                     HTGRAMPGRRWSEGIHQAIEAKERVAIQNETQTLASTTFQNYFRLYDKLAGMTGTADT
                     EAFEFRQIYGLDVVVIPTHMPMVREDNNDLVYLTIEEKYDAIVEDINDCIERGQPVLV
                     GTASIESSERLSGELDRRKIAHNVLNAKQHEREADIIAQAGSPGAVTIATNMAGRGTD
                     IVLGGNWEAEAAQLDSPTDEELQRLRGEWQKRHDAVIAAGGLHIIGTERHESRRIDNQ
                     LRGRSGRQGDPGSSRFYLSMEDNLMRIFASDRIRGIMQSIGLEKGEAIEHRMVNNAIE
                     KAQRKVEGHNFDIRKNLLEFDDVANDQRQVIYQQRRELMVADDIAADIEGLREEVVLD
                     LIAEYIPPQSIEEQWDVDGLEQALQTEFASRQPVRGWLDEDDALNEDSLSRKILAEVE
                     AEYRAKEQDWASHGIDMRVVEKQIMLQVLDQRWKEHLAAMDYLRQSIHLRAYAQKQPK
                     QEYKRESFQLFQELLENIKRDVIRLLCRVQIETSDELEEAERRRREEAERRMRYTHAE
                     SSGLDGGEQEQPDDGAGKPETFVRSERKVGRNEPCPCGSGKKYKQCHGKAA"
     gene            complement(7432..8340)
                     /locus_tag="AC16QF_16425"
     CDS             complement(7432..8340)
                     /locus_tag="AC16QF_16425"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR6450197.1"
                     /translation="MKIILLRSSGGAHALRVPRWMGALALAIGLVATAAGAVLGMNLL
                     GSHQMAPETVVHWRQEVEAQRLELQALLDRSTAETQAVGRQLAQMQARLLRMEALGAR
                     VAEVADLDDGEFSFDLPVPVGGPDTTTSEPMPMGDLRADVDALARNLKIRETELEILE
                     SMLRDRDYRAEVAVAGRPVTWGWISSSYGERVDPFSGKMAWHAGLDFAGKQGSDVIAV
                     ASGVVTHAGNRFGYGEMVEVTHGDGYVTRYGHHAEIVVAVGEIVKKGQVVGKMGSSGR
                     STGPHVHFEVLKNGRRVDPARYVVRR"
     gene            8454..8762
                     /locus_tag="AC16QF_16430"
     CDS             8454..8762
                     /locus_tag="AC16QF_16430"
                     /inference="COORDINATES: protein motif:HMM:NF017103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HIR6450198.1"
                     /translation="MANLKVDLLLSESQRRFTPLQRLLRQSANQESWTAGLLALLPAS
                     LQNDCRVSDIRNGSIIVVCRNAATATRLRFLAPELLSKLHDLADFRVAQRIEVRVSEF
                     "
     gene            complement(8863..9777)
                     /gene="lpxC"
                     /locus_tag="AC16QF_16435"
     CDS             complement(8863..9777)
                     /gene="lpxC"
                     /locus_tag="AC16QF_16435"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103117 - UDP-3-O-acyl-N-acetylglucosamine
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HIR6450199.1"
                     /translation="MALNQKTLKNSIRATGVGLHTGDKVFLTLRPAPVDTGIVFVRTD
                     LDGEPRLPARVENVGDTTMATSLTNGTVSVSTVEHLMSAFAGLGIDNAFVDVSAPELP
                     IMDGSAAPFVFLIQSAGVEEQTAPRRFVRIKKAVAFRQGDVVAELKPFDGFRVSYTLV
                     YDHPVFRKHERSATVDLSNTAYVKEVSRARTFGFLADYEQLRSMNLARGGSLDNAVVV
                     DDYRILNGEGLRQADEFVKHKILDAVGDLYLLGCGLIGEFCGYKSGHGSNNALLRRLL
                     ADPEAFEIVTFANEADAPTGFAAPVVAN"
     gene            complement(9980..11128)
                     /gene="ftsZ"
                     /locus_tag="AC16QF_16440"
     CDS             complement(9980..11128)
                     /gene="ftsZ"
                     /locus_tag="AC16QF_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR6450200.1"
                     /translation="MFELVDSAPQSAVIKVVGVGGGGGNAVQHMLSRQVEGVDFIAAN
                     TDAQALKHLDAKTILQIGSNITKGLGAGANPDIGRQAAIEDRDRLGEVLVGADMVFIT
                     AGMGGGTGTGAAPVVAEVARELNILTVAVVTRPFKFEKRSDVADRGIAELRETVDSLI
                     TIPNEKLLAVLGERTTMLDAFGAANDVLLGAVQGIADLIIRPGIINVDFADVRTVMSE
                     MGMAMMGTGRGTGDNRARDAAEAAIRSPLLEDVDLHGARGILVNVSAGPDLTIGEYSD
                     VGDIINEFASEQATVVVGTVIDQDLGDELKVTVVATGLGEPARPNVVIDNTVIEEVIQ
                     GQIDYRDLDQPTYKRHNQALHKDNLAEDLDTQDLDYLDIPAFLRRQAD"
     gene            complement(11142..12371)
                     /gene="ftsA"
                     /locus_tag="AC16QF_16445"
     CDS             complement(11142..12371)
                     /gene="ftsA"
                     /locus_tag="AC16QF_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR6450201.1"
                     /translation="MASDSELTRIVGLDIGTSKVAAIVAELNLEGELEIIGLGTHPSR
                     GLKKGVVVNIESTVQSIQRAVEEAELMAGCHIENVYAGIAGSHIRSINSHGIVAVRDR
                     EVYPQDVDRVIDAAQAMAIPADQKILHILPQEYVIDNQEGVKEPLGMSGVRLEAKVHL
                     VTCAVNAAQNIEKCIVRCGLTVNDIILEQLASSYSVLTDDERDLGVCLVDIGGGTTDI
                     AVFTGGAIRHTAVIPIAGDQVTNDIAMALRTPTQNAEEIKIRYACALTQLAGPDETIK
                     VPGVGDKPAKELSRQALAEVVEPRYDELFTLIQAELRRSGFEDLIAAGIVLTGGASKM
                     EGVVELAEEIFHMPVSLGAPRDVAGLKDIVRNPVYATGVGLLQYGREREREMPGRGRL
                     RGKGAFGRMKKWLSDNF"
     gene            complement(12361..13065)
                     /locus_tag="AC16QF_16450"
     CDS             complement(12361..13065)
                     /locus_tag="AC16QF_16450"
                     /inference="COORDINATES: protein motif:HMM:NF015739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR6450202.1"
                     /translation="MRRVSAVLLIVLAGAVVAALVWQMDRPIENVRVIGELTPGEQQQ
                     IEAAVLTGLDAGLLSVDLAEVRDRLRSLSWTRRVTVRRVWPPGLDIAVDKDVPVAVWG
                     DGAHLTSNGRVIAIPDAPAALPVFDCALASPRSALEVYLLLQESLPGAGRRISALRQT
                     SLGEWEVELRGGLRVALGDRDIAQRIARFGIVYERALKDRIETVDYVDARYTGGLAVG
                     WKDPLLAYEEPTANGI"
     gene            complement(13058..14446)
                     /gene="murC"
                     /locus_tag="AC16QF_16455"
     CDS             complement(13058..14446)
                     /gene="murC"
                     /locus_tag="AC16QF_16455"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR6450203.1"
                     /translation="MRRVRRIHFVGIGGAGMCGIAEVLLNQGYQVSGSDLRASETTAR
                     LERLGATVHVGHAAGHVEGKDVVVVSSAVAGDNPELRAAHARRIPVVARAEMLGELMR
                     YRHGIAVAGTHGKTTTTSLITAIFQTAGLDPTFVIGGLLNSTGTNAQLGAGRYLIAEA
                     DESDASFLHLKPMQAVITNIDRDHMGTYHNDFAELRGAFVRFVHRLPFYGSVVLCTDD
                     ANVRDVLPELARPMLTYGFAEDGDYRARDLDVNGASWRFVAERPDGHGDLEISLAVPG
                     EHNVLNALAAAAVATDEGIPDEAIVAGLSQFRGVGRRFEVFAQTPVGCARVTMVDDYG
                     HHPSEVAAVIQTARRVWPDRRLVMVYQPHRFTRTRDLYDDFVRVLSEVDALVLLEVYA
                     AGEEPIPGADGKALCQGLRQRGLVNPTYAADPEEALGLLPDIVRDGDVVLVQGAGSVN
                     QISLSLKEVTDA"
     gene            complement(14466..15551)
                     /gene="ftsW"
                     /locus_tag="AC16QF_16460"
     CDS             complement(14466..15551)
                     /gene="ftsW"
                     /locus_tag="AC16QF_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIR6450204.1"
                     /translation="MTARLDMTLLLSWLLILVLGTVMVASAAVAISESLVWKHGIFLL
                     VSLTAFGVVLAVPLAVWQRCHHLAWGLAVILCILVLVPGIGVEVNGARRWIGLGAFTL
                     QASEFAKLLVLVYVAGYLARFDEAVRESSIALLRPLMMVAIVALLLLMEPDFGSVVVL
                     GAAVCGMLFVAGTRLRHFLLLVVVAALLLAALALLQPYRMQRLVSFTDPWSFAFGSGY
                     QLTQALIAFGRGEVTGLGLGEGIQKLFYLPEAHNDFIFAVIAEELGLLGTVAVVVLFG
                     TLIARVLLIAGRAVAEERYFAGYLTYGVGLLLGVQCLVNMGVNTGMLPTKGLTLPFVS
                     FGGNSLLVCSTMLGLAFRAQMEAKGRG"
     gene            complement(15548..>15875)
                     /locus_tag="AC16QF_16465"
     CDS             complement(15548..>15875)
                     /locus_tag="AC16QF_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR6450205.1"
                     /translation="ESSGGSLILIAGGDGKGADFTALREPVSRYVKTAVLLGVDADAM
                     ARALDGAVPLVRVDTLEAAVVAARNAAGPGDLVLLSPACASLDMFENFADRGERFAAA
                     VMEFAA"
BASE COUNT         2818 a         5337 c         4902 g         2818 t
ORIGIN      
        1 gcccaaggaa acgctcaagg cagaagggtt tacagatgag cagatcgacg agggtttcga
       61 gctcttcgag cccgtgggct gtgacagctg caacaatggc tacaagggcc gagtcggaat
      121 ttatgaagta gttaaaatta cgccagaaat gtcccgtatt atcatggagg acggaaacag
      181 cattaagctg gcggaaactg caagcaaact cggcttcaaa aatctccgcc aatcaggtct
      241 gctgaaagta atacaaggcg tgaccagtct ggccgaagcg aacaggatga cgacggggca
      301 ctgacacatg gccgaaagcg caatcttcct ctgggaaggc atagacaaag gcggtcgcaa
      361 gaccaagggc gagatatcga gctccacacc ggccatcgcg aaagcggaac tgcgccgtca
      421 gggcatcaac gccaccaagg taaagaagaa gagcaaggcc ttttcgctgg gcggaggcgg
      481 cagcgtcaag cctgtcgaca tcgccttgtt cacgcggcag atgtccacga tgatgagagc
      541 cggcgtgccg ctggttcagg cattcgagat cgtggcaggc ggggtcgaca agcccaaggt
      601 cgctaccctc atccgggcac tgcgcgacga cgtggcaggc ggcagctcgt tcaccgcagc
      661 gctgcgcaag catcctctgc acttcgacga ccttttctgc aacctggtcg gagccggtga
      721 gcaatccggt gctctggaaa ccatgctcga ccgcatcgcg acgtacaaag agaaaacgga
      781 gtccctgaag gccaaggtcc gcaaggccat gacctacccg gccgccgtcc tggtggtggc
      841 gttcatcgtt tcaggcatcc tgctcatcaa ggtggtgcct caattcgagg aggtcttcgc
      901 aggtttcgga gcggagctgc ccgccttcac ccagttcgtg ataaacatct cggagttcac
      961 gcagcaatgg tggtttctga tcgtcgttgc tatcggcggc accatcgcca ccttcatggc
     1021 cgcgcgaaaa cgctcgaaga aactccgcga ggcgatagac aggattgcgt tgaaagcacc
     1081 catcttcggc aacatcatcg agaaatccgc cgtggcccgt tacgcacgta cgctggccac
     1141 caccttcgcc gccggcgtac ctctggtaga ggctctgaat tctgtagccg gatccaccgg
     1201 caactccgtt tacaccaagg cggtttacca gatacgcgat gacgtgagca ccggtcagca
     1261 gctgaacttc tccatgcgct ccaccggcgt gtttcccaac atggtggtgc agatggtcgc
     1321 catcggcgaa gaggccggcg cgctggacga gatgctggat aagtcggcgt cctattacga
     1381 agagcaggtc gacaacgccg tggacaacct caccgcgctc atggagccga tgatcatggc
     1441 cgttctcggc gtgctggtcg gcggcctcat catcgcgatg tacctgccca tcttccagct
     1501 gggtcgggtc gtcgggggtt aacccaggct cggcacggcg aatcgtatgc gaccgggcta
     1561 gcgctcacgt catgggggaa acacttcttg cctacggatg ggccggcctc acctttgcgg
     1621 tgtgggtggg cctgtcggta ggcagcttcc tcaacgtggt catctaccga ctgcccatca
     1681 tgctctcacg gcaatgggag gcgcaggcgc gcgaactcct cgaagaggaa ccagcggaaa
     1741 ccgggcagga gcgtttcaac ctcatggtgc cgcgttcgcg ctgccccgcc tgtcagcagc
     1801 ccatctcggc gtggcagaac gttccggtct tcagctggct ctttttgaaa ggccgctgcc
     1861 gaagctgcgg cgtcagaatc tccatccgct accccatggt ggaggtgctc accggggccg
     1921 cgaccataac ggtgctggcg ctcttcgggt acacgtggct gggtctagcc gccgctgtgt
     1981 acacctgggc aatgatcgcg ctgacgttca tcgactacga cacccagctg cttcccgacc
     2041 agatcacgct gccgctgctc tggctgggcc tgatcacctg ccacttcggc ggcgtcacgg
     2101 atctgggctc cggggtcctc ggcgccgtga tcggctacct gtttctctgg acgaccttct
     2161 gggctttcaa gctcatcacc ggcaaggagg gcatgggcta tggcgacttc aagctcttcg
     2221 cggccatcgg agcgtggctc ggatggcagg tgctgccggg cacgatcctc atcgccgcga
     2281 cgctggggct cgcgtatgcc ctggcgatca cgctggccgg caaacgggag gcagcgcagc
     2341 cgatcccatt tgggcccttc ctggcggttg cgggctgggt ttcgcttatg gcccgtgata
     2401 acgtgctgac cctgtttgca gcctgaccgg tcctccgacc tcaaacgaca tcccaatgag
     2461 cagactggtg atcggaatca ccggcggcat cggcagcggc aagaccgccg tctccgaccg
     2521 gttcaaagcg ctgggggtaa ccgtcgtcga cgccgacatc gcctcccgcg tgatagtcga
     2581 gccaggccga ccagccctcg tcgccatcgc cgagcacttt ggctgcgatg tcatacaggc
     2641 ggacggatca ctcgatcggg cggagcttcg acgcagggtg ttcgcagacc ccgacgagcg
     2701 ccgctggctg gagcagctca cccacccgct gatcaacgag cacatccgcg aggagcttga
     2761 acaggccgag tctccctacg ccgtgctcgt cagcccgctg ctggccgaga ccggccagtc
     2821 gcgattctgt catcgcattc tggtggtgga cgtgccggag gaagtacagg tgcggcgaac
     2881 catggcccgg gacgccaacg acgaggccca ggtgagaaag atcatcgagg cccaggcatc
     2941 ccgcgagcag cgcctggccc tcgccgacga cgtcatcgtc aacgactcga gcctggaggt
     3001 gctggacgag gaggtggcgc gcctgcacca ggtctacacg gagatggcgc aatgaccgcc
     3061 tccgaacccg accggaccgc gcgcatcgtc acctgcccga cctgcggggg ccgcgtcaaa
     3121 tggacgtccg gcaacgagtt ccgacccttc tgctccgagc ggtgcaagct catggacctc
     3181 ggcgcctggg ccagcgagca gcacagaatt ccgggcaacg aggtggacga cgatctgctc
     3241 tcgggggatc tggacccgga ctgaccggcc aggccgagaa gcttccatgg atcaggtgcg
     3301 atactcagcg ttgatgcgca cgtactcgta ggacagatcg gttgtccaga ccgtctcctc
     3361 ggcgctgccg cggccgaggt cgacgtggac ttcgaactcc tgccttgcca tcaccctggc
     3421 agcggcggct tcctcgtact gcggcgccac gagcccgccc agggcgatgg gcacgtcgtc
     3481 caggtgaacg gcgacgcgac cggtgtcgag atcggcaatg cccgccctgc cgatggccat
     3541 gcagaaccgc ccccagttcg gatcgccggc aaatatcgcc gttttgacca gaggggactc
     3601 ggctaccgtg taggccacct tgagacattc ggccgcactc tcgccgccgg agacgcgcac
     3661 ggtgacgaat cgcgtggctc cctcgccatc gcgaacgatg ctctgagcca gctcggtagc
     3721 caccgcggcc aggccatcgc gaagctccgc gtagccttcg gagccgacgt cggtcacggg
     3781 cgcgttctcc gcgcatcccg tcgcgcagac cacgaaggcg tcgttggtgg acgtgtcgcc
     3841 atccacggtg attcgattga acgagcggcc ggccacttcc ctcaccatcg cgggaaggca
     3901 gccgtcggcg acggcagcgt cgcagcagac gaagcccagc atcgtggcca tgtcgggctt
     3961 gatcatgccc gctccctttg ccatgccggt gatcaccacc tcttcgcccg cgaccctgac
     4021 gcgacggctg acggccttgg gcgccgtgtc cgtggtcatg atggcgcgag cggcggcctc
     4081 ccagccgtcc ggcgccaggc tcccacaggc ggaaacgacc gccggcagca ttctggaaac
     4141 ggggagacgc tcaccgatga ccccggtgga gaacggcagc acggaatcac cgggcacatc
     4201 cagggcggcg gcaaccgcat cgcagacggc aacggcatcg gcaagaccgg gatccccggt
     4261 ggccgcgttg gcgttaccgc tgttgatcac cagagctcgc gcgcgcccgc cggaaagtcg
     4321 ccgatcggcc agggtcacgg gagcggctcg aaacgagctg cgcgtgaaca cgcccgccgc
     4381 cctggtctgg gggtgcagca gcaggacggc gatgtcgtca cggccctgtc tcttgatggc
     4441 agcccctgca gtacccaaag acacaccggg gaccaccagc agttcctgca gcggaggaac
     4501 gttcaccgcc atgtcgggct tccccctacg cagccttgcc gtgacactgt ttgtacttct
     4561 tgcccgagcc gcacgggcaa ggctcgttgc ggcccacctt ccgctcactc ctcacgaagg
     4621 tctcgggttt gccggcgcca tcgtccggct gctcctgctc acccccgtcg agcccgctgg
     4681 actcggcatg ggtgtaacgc atgcgacgtt cggcctcttc gcgccgacgg cgctcggctt
     4741 cctccaactc gtcggaggtc tcgatctgca cccggcagag caggcggatc acgtcccgct
     4801 tgatgttctc cagcagttcc tggaacagct gaaagctctc ccgcttgtat tcctgcttgg
     4861 gctgcttctg ggcgtaggca cgcagatgaa tgctctggcg caggtagtcc atggccgcca
     4921 gatgctcctt ccagcgctgg tccagaacct gcagcatgat ctgcttctcg accaccctca
     4981 tgtcgatccc gtgggacgcc caatcctgct ccttggcgcg atactccgcc tcgacctcag
     5041 ccaggatctt gcgcgacaac gaatcctcgt tgagcgcgtc gtcctcgtcg agccagcctc
     5101 tcaccggctg cctgctggca aactcggtct gcagggcctg ctccaggcca tcgacgtccc
     5161 actgctcctc gatgctctgc ggagggatgt actctgcgat caggtccaga acgacctctt
     5221 cgcgaagccc ttcgatgtcg gccgcgatgt cgtccgccac catcagctcc cgtcgctgct
     5281 gatagatcac ctggcgctga tcgttggcaa cgtcgtcgaa ctccagaagg ttcttgcgga
     5341 tgtcgaagtt gtggccctcg accttccgct gcgctttctc gatggcgttg ttcaccatgc
     5401 gatgctcgat ggcctctccc ttctcgagcc cgatggactg catgattccc ctgatgcggt
     5461 ccgatgcgaa tatccgcatc aggttgtctt ccatggaaag atagaagcgg gacgagcccg
     5521 ggtcgccctg acgccccgac cggccgcgga gctggttgtc tatgcgccgc gactcgtggc
     5581 gctcggtgcc gatgatgtgc aggccaccgg cagcaatgac cgcatcgtgg cgcttctgcc
     5641 actcgccccg caggcgctgc agctcctcat cggtaggcga atccagctgg gccgcctcgg
     5701 cttcccagtt gccgccgagc acgatgtccg tgcctcgacc ggccatgttc gtggcgatgg
     5761 tcaccgcgcc cgggctgccg gcctgggcaa tgatgtcggc ctcgcgctcg tgctgcttgg
     5821 cgttgagcac gttgtgagcg atcttccggc gatcgagctc cccagacagc cgctcggaag
     5881 attcgatgga ggcggtcccc accagcaccg gctggccgcg ctcgatgcag tcgttgatgt
     5941 cctcgacgat cgcgtcgtac ttttcctcga tggtgaggta aaccagatcg ttgttgtctt
     6001 cccgaaccat gggcatgtgg gtggggatca cgacgacgtc caggccgtag atctgccgga
     6061 actcgaacgc ctcggtgtcg gcggtgcccg tcatgccggc aagcttgtcg tagagccgga
     6121 agtagttctg gaacgtggtc gaggccaggg tctgggtctc gttctgaatc gccacccgtt
     6181 ccttggcctc gatggcctga tgaatgccct cggaccagcg cctgcccggc atggcccgac
     6241 cagtatgctc atcgacgatg accacctcac cgttggccac catgtaatcc acgtcccgct
     6301 tgaacagggc gtgtgccttg agtgccgcat gcacgtgatg cagcaggttc aggtttgcgg
     6361 ccgcgtaaag gctgtcgcct tcctcgagga ggccgagccg catgagctcc gcctccacct
     6421 tctcatggcc gacctcggtc agctccacct ggcggttttt ctcgtccacc acgaaatcgc
     6481 ccgtcggctc ggcttccgga tcctcgaccg gcggttgctg cttgagcttg ggcgcgatcc
     6541 ggtcgatctt ctgataaagc tccgtgctct gctcggcggg gccggagatg atgagcggcg
     6601 tgcgggcctc gtcgatgaga atggagtcca cctcgtcgac aatggcgtaa ccgaggtcac
     6661 gctgaaactt gtcggccagc gagaacgcca tgttgtcgcg caggtaatcg aagccgaact
     6721 cgttgttcgt gccgtaggtc acgtccgact gataggcttc ccgcttctct tgcggcgcct
     6781 gacccgactg gatcacgccc accgtgaggc cgagcgtctc gtagatcggt cccatccact
     6841 ccgcgtcgcg tcgtgcaagg tagtcgttga cggtcaccac gtgcaccccg cgaccggcca
     6901 gggcgttgag gtaggcggcc agcgtcgcca ccagggtctt gccctcgccg gtgcgcatct
     6961 ccgcgatgcg cccctcgtgg agggtgatcc caccaatcag ctggacgtcg aaatggcgca
     7021 tgtccttggc gcggcgggca gactcacgca ccgcggcaaa cgcttccggg agcaggtcct
     7081 ccagactggc gccctgctcc agccgttcct tcagccggcc ggtggtggcc ttgagttcgt
     7141 cgtcggaaat ctcgagcagg tcggcctcga gcgcgttgat ccgggccacg atcctgccca
     7201 tccgcttgag ctctcgcgcg ttcttggtgc cgaaaatttt cttgagcatg tgctttcgcc
     7261 tgggtgccgg atgcgaaacc ggcggcagtt taccctaatt cgaccacaat ccgcgggaga
     7321 cgatcgcttc atccgatgaa ggatggagcc tgcccgacaa ggcagcgaag gtcggctggg
     7381 ttccgggcct gccggcgcgt gtcgagacca gatcggggct gccggatcgc gtcagcgacg
     7441 tacgacgtaa cgggctggat cgactcgacg gccgttcttc aaaacctcga aatgcacgtg
     7501 cgggccagtg gaacggccgc tggagcccat ctttccaacg acctggcctt tcttgacgat
     7561 ctcgccgacg gcaacaacga tttccgcgtg gtggccgtat cgggtgacgt acccgtcgcc
     7621 gtgggtgacc tcgaccatct ctccgtaccc gaagcgattg cccgcatggg taaccacgcc
     7681 gctggccacc gcgatgacgt cagaaccctg tttgccggcg aagtccaatc ccgcgtgcca
     7741 ggccatcttg ccgctgaacg ggtccacccg ctcgccgtac gaggaggaga tccagcccca
     7801 cgtcaccggg cggccggcca cggccacctc agcccggtag tcgcggtcgc gcagcatgga
     7861 ttcaagaatc tccagctccg tttcgcgaat cttcagattc cttgccagag cgtccacgtc
     7921 ggcgcgcaga tcccccatcg gcatcggctc cgaagtggtc gtgtccgggc caccaacggg
     7981 aacgggcaga tcgaacgaga actcgccgtc gtccaggtcg gccacttccg ccacgcgggc
     8041 tccgagcgcc tccatgcgca gaagccgtgc ctgcatctgg gcaagctgtc ggccgacggc
     8101 ctgggtttcc gcggtggatc gatcgagcag ggcctgcagc tcgaggcgct gagcctcgac
     8161 ctcctggcgc caatggacga cggtttccgg ggccatctga tggctcccga ggaggttcat
     8221 cccaagcacg gcgcccgcag cggtcgctac gaggccgatg gcaagcgcca gcgcccccat
     8281 ccagcggggc acccgcagcg catgagcacc acccgagctt ctcagcaata tgatcttcac
     8341 cgtcgcgata cccccctgac cgcaacccga aacggtggcg aaacgaaccg ccccgggcca
     8401 aaatccatag cccgagggcg gccgacacgg tcgttcatga tcgagacccg gccatggcga
     8461 atctcaaagt cgatcttctg ttgagcgaaa gccaacgccg atttactcct ctccaacgtc
     8521 tcctacgtca gtcggcgaat caagagtcct ggacggccgg gctgttagcc ctgctgcccg
     8581 cttcgctgca gaacgactgt cgcgtcagcg acatccgcaa cggctcgatc atcgtggtct
     8641 gccgaaacgc tgcaaccgct acgcggttgc gattccttgc gcctgaactg ctgagcaaac
     8701 ttcacgatct agccgatttc cgagtggctc agcgaatcga ggtgcgcgtc agcgaattct
     8761 gacaggcgcc cggttccttg tgccctcggc tgtgacgatt atcccctcga ccccagcctc
     8821 attttgagac atgtcggtca aattggccaa tgagtctgcg aatcaattag caaccacggg
     8881 agcggcgaag cccgtcggcg cgtccgcttc gtttgcgaag gtaacgatct cgaaagcctc
     8941 ggggtcagct agtaacctgc gcaaaagcgc attgttggat ccatggcctg atttgtagcc
     9001 gcagaactcg ccgatcaggc cacagccgag caggtagagg tcgcccacgg cgtcgagaat
     9061 cttgtgcttg acgaactcgt cggcctgccg gagcccctcc ccgttgagga tgcggtagtc
     9121 gtccaccacc accgcattgt cgaggctccc tccccgcgcg aggttcatgg atcgcagctg
     9181 ttcgtaatcg gccagaaaac cgaacgtacg cgcccgcgac acctccttga cgtaggcagt
     9241 gttggaaaga tccaccgtgg ctgagcgttc gtgcttgcgg aataccgggt ggtcgtagac
     9301 cagggtgtag gaaacgcgga acccgtcgaa cggcttgagc tccgcgacaa cgtctccctg
     9361 ccggaacgcc accgccttct ttatgcgaac gaatcgtctg ggcgcggtct gttcctctac
     9421 cccggcggac tggatgagaa acacgaacgg cgcggcgctg ccgtccatga tcggcaactc
     9481 cggggcgctg acgtccacga aggcattgtc gatcccgagc ccggcaaacg ccgacatgag
     9541 atgctcgacc gtggaaacgc tgacggtgcc gttggtcagc gacgtcgcca tggtggtatc
     9601 gcccacgttc tccacccgcg ccgggagcct cggctcgccg tcgagatcgg tgcgcacaaa
     9661 gacgattccc gtatccacgg gggccgggcg cagggtcaga aagaccttgt ctcccgtatg
     9721 cagaccaacg ccggtggcgc ggatcgagtt cttgagcgtt ttctgattca gcgccatgtt
     9781 gcccgtcctt caggccaggg cccctgcaag aagccgcgaa tcgcgctcct tcttcctctt
     9841 ttacgcaaag cgctccccgc gcacacgccc tttcgagcct gtgcaccgac cggcacccgg
     9901 cagaagggga tcatctgccg gccggcgcta aattatggca gccggcagcc ggtaagtcac
     9961 acgtttcacc cgggcacgtt cagtcggcct ggcgacgcag gaaagccgga atgtccagat
    10021 agtccagatc ctgagtgtcc agatcctctg caaggttgtc cttatgcaga gcctggttgt
    10081 gacgcttgta agtcggctgg tccagatccc ggtagtcgat ctgcccctgg atgacctcct
    10141 cgatcaccgt gttgtcgatg accacattgg gtcttgccgg ctcgccgagc cccgtggcca
    10201 ccacggtgac cttcagctcg tcgcccagat cctgatcaat gaccgtcccc accacgacgg
    10261 tggcctgctc ggaggcaaac tcgttgatga tgtcgcccac gtcggagtac tcgccgatgg
    10321 tgagatccgg cccggcagag acgttgacca gaatgcctct cgcgccgtgc agatccacgt
    10381 cctcgagcag aggcgagcgg attgccgctt ccgccgcgtc gcgcgcgcga ttgtctcccg
    10441 ttccccggcc cgtgcccatc atggccatgc ccatctccga catgaccgtc cgcacgtcgg
    10501 caaaatccac gttgatgatt cccggccgga tgatgaggtc ggcaatcccc tgcacggccc
    10561 cgagcagcac gtcgttggcc gcaccgaacg catccagcat ggtggttcgc tccccgagca
    10621 cggcaaggag cttttcgttg ggtatggtga tcagggaatc gacggtctca cgcagctccg
    10681 cgatcccgcg atcggcgacg tccgagcgct tttcgaactt gaaaggccgg gtgaccaccg
    10741 cgacggtcag gatgttcagc tcacgggcca cttccgcgac cacgggcgca gctccggttc
    10801 ccgtgccgcc tcccatcccg gcggtgatga acaccatgtc cgccccgacc agcacctcac
    10861 ccagacgatc ccgatcctcg atggcggcct gccgcccgat gtcgggattc gcgccggcgc
    10921 cgaggccttt ggtgatattg ctcccgatct gcagaattgt cttcgcgtcg aggtgcttca
    10981 gagcctgggc atcggtattg gcggcgatga aatccacccc ctctacctgc ctgctcaaca
    11041 tgtgctggac ggcgttgcca ccgccgcctc ccactccgac gaccttgatc accgcgctct
    11101 gcggggcact gtctaccagt tcaaacatcc tgatctccta actagaaatt gtcgctcaac
    11161 cactttttca ttcggccgaa agcacccttg ccccgaagcc ttccgcgacc gggcatctcc
    11221 cgctccctct ccctgccgta ctgcaacagg ccgacgccgg tcgcgtatac gggatttctc
    11281 acgatgtcct tcagacccgc cacgtcacgt ggcgcgccca ggctgaccgg catgtggaag
    11341 atctcttccg caagctcgac aaccccctcc atcttcgacg ctcccccggt gagcacgatg
    11401 cccgcggcga tgaggtcttc gaacccgcta cgccgcagct ccgcctgtat cagcgtgaac
    11461 agctcgtcgt agcggggctc caccacctcc gcaagcgcct ggcgcgacag ctctttggct
    11521 ggcttgtcgc cgaccccggg aaccttgatg gtttcatcgg gaccggcgag ctgcgtcagc
    11581 gcgcaggcat atctgatctt gatctcctct gcgttctgcg tgggcgttcg cagcgccatg
    11641 gcaatgtcgt tggtgacttg gtcaccggcg atggggatca ccgccgtgtg acgaatggcg
    11701 ccgcccgtga acaccgcgat gtccgtggtg ccgccgccta tgtccaccag gcagacgccc
    11761 aggtcgcgct cgtcgtccgt aagcacggag tagctcgagg caagctgctc gagaatgatg
    11821 tcgttgacgg tcaggccaca ccgcacgatg cacttctcga tgttctgcgc ggcgttcacg
    11881 gcgcaggtga ccagatgcac tttggcttcg agccgcaccc ccgacatccc gaggggctct
    11941 ttcaccccct cctggttgtc gatgacgtat tcctggggaa ggatgtgcag aatcttctgg
    12001 tccgccggga tggccatggc ctgggccgca tcgatcaccc ggtccacgtc ctggggatag
    12061 acctcgcggt cccgcacggc gacgatcccg tgggagttga tgcttcgtat gtgactgccg
    12121 gcgatgcccg cgtagacgtt ctcgatgtgg cagccggcca tgagctcggc ctcctccacg
    12181 gcgcgctgga tggactgcac ggtggattcg atgttcacca ccacgccttt cttcaatccc
    12241 cgtgatgggt gcgttccgag cccgatgatc tcgagctccc cttcgaggtt gagctcggcc
    12301 acgatggcgg ccaccttgct cgtgccgatg tcgagaccga cgattcgggt cagctcactg
    12361 tcagatgcca tttgctgtgg gttcctcgta tgcaaggagc ggatctttcc aaccaaccgc
    12421 gagaccgccg gtataccgcg catcgacgta gtcgacggtc tcgatgcggt ccttcaacgc
    12481 ccgttcgtag acgatgccga atcgggcgat tcgctgggcg atatcccggt cgcccagcgc
    12541 aacccgaaga ccgccgcgaa gctcgacttc ccactcgccg agggacgtct gcctgagcgc
    12601 cgagatgcgt cttcccgcgc ccggcaggga ctcctgcagc agcagataga cttccagcgc
    12661 gctgcgcggg ctggccagcg cgcagtcgaa gacgggcagc gcggcgggcg cgtccgggat
    12721 cgcgatcacc cggccgttgc tggtgaggtg agcaccgtcg ccccagaccg cgacaggaac
    12781 atccttgtcg acggcaatgt cgagccccgg aggccatacg cggcggaccg tcacgcgacg
    12841 cgtccaggaa aggctccgca gccggtcacg cacctcggcg agatccacgc tcagcaggcc
    12901 cgcatcgagg ccggtgagca ccgccgcctc gatctgctgc tgctccccgg gggtgagctc
    12961 gccgatgacg cgcacgttct cgatgggccg atccatttgc cacaccagcg cagccaccac
    13021 ggccccggcc agcacaatca ggagcacggc ggacacccta cgcatccgtc acctccttga
    13081 ggctcagcga gatctggttg acgctgccgg ccccctgaac gagcaccacg tcgccatccc
    13141 ggacgatgtc gggcaggagc cccagggctt cctcggggtc ggcggcgtag gtggggttga
    13201 cgagcccgcg ctgccgcagg ccctggcaca acgccttgcc gtccgcgcca ggtatgggtt
    13261 cctcgcccgc cgcgtaaacc tccagcagca cgagggcgtc cacctcggag agaactctca
    13321 cgaaatcgtc gtaaagatcc ctggtacggg tgaaccggtg cggctgatag accatgacga
    13381 gccgccgatc gggccagacg cggcgcgcgg tctgaatcac tgcggccact tcggaaggat
    13441 ggtggccgta gtcgtcgacc atcgtgacgc gggcgcagcc caccggcgtc tgcgcgaaaa
    13501 cctcgaaccg ccggcccacg ccgcggaact gcgacaggcc ggcgacgatg gcctcgtccg
    13561 gtatgccttc gtcggtggcg accgctgccg ccgcgagcgc gttgagcacg ttgtgctcgc
    13621 cgggaacggc cagggagatc tccaggtcac cgtgaccgtc gggacgctcg gcgacgaaac
    13681 gccacgatgc gccgttcacg tcgagatccc gggcgcggta gtcgccgtcc tccgcgaagc
    13741 cgtaagtcag catgggccgg gcaagctcgg gcagaacgtc gcgcacgttc gcatcgtccg
    13801 tgcacagcac gacgcttccg tagaagggca gacggtgcac gaaccgcacg aacgcgccgc
    13861 gcagctcggc aaagtcgttg tggtaggtgc ccatgtgatc ccggtcgatg ttggtgatca
    13921 cggcctgcat gggtttgagg tgaagaaacg acgcgtcgct ttcgtcggct tccgcaatca
    13981 ggtaccggcc ggcgcccagc tgcgcattgg ttcccgtgct gttgagcaaa ccgccgatga
    14041 cgaaggtggg gtccaggccc gcggtctgga atatggccgt gatcaggctg gtagtggtcg
    14101 tcttgccgtg ggttccggcg acggcaatgc cgtgacgata gcgcatgagc tccccgagca
    14161 tctccgcccg cgccacgacc ggtatccgcc gcgcgtgagc cgcgcgaagc tcagggttgt
    14221 ctcccgcgac cgcgctggat accacgacca cgtccttgcc ctcgacgtgc cccgcagcat
    14281 ggcccacatg gacggttgcg ccgagccgtt ccagtcgcgc cgtggtctcc gacgcgcgaa
    14341 ggtctgatcc cgacacctgg tatccctggt tgagaagcac ctcggcaatg ccgcacatgc
    14401 cggcaccgcc aatgcccacg aagtgaatgc gccgcacccg gcgcatctcc ggcacgtagt
    14461 acgactcagc cacggccttt cgcctccatc tgggcccgga acgcgagccc cagcatggtg
    14521 ctgcacacca gcaggctgtt accaccgaag ctcacgaatg gcagcgtgag gcctttggtc
    14581 ggcaacatcc cggtgttgac gcccatattg acaaggcact gcacgcccaa cagcaggccg
    14641 acgccgtagg tgaggtagcc ggcgaagtat ctttcttccg ccaccgcccg accggctatg
    14701 agcagcaccc tggcgatcag cgtgccgaac agaacgacca ccgcgacggt gcccagcagg
    14761 ccgagctcct cggcaatcac ggcgaatatg aagtcgttgt gcgcttccgg caggtagaag
    14821 agtttctgga taccctcgcc aagaccgagc ccggtgacct ctccacgccc gaaggcgatc
    14881 agcgcctggg tgagctggta gccgctgccg aaggcgaacg accacgggtc cgtgaagctc
    14941 accagcctct gcatgcggta gggctgcagc agcgcaaggg cggccagcag cagagccgcg
    15001 accacgacga gcaggagaaa gtgacgaagc cgggtgcccg cgacgaagag catcccgcag
    15061 acggcggcgc cgagaaccac gacggagccg aagtcgggct ccatgagcag cagcagcgcg
    15121 acgatggcca ccatcatgag cggccgcagc agcgcgatgg agctctcccg caccgcttcg
    15181 tcaaagcggg ccagatagcc ggccacgtag accagcacca ggagcttggc gaactctgac
    15241 gcctgcagcg tgaaggcgcc aagccctatc cagcgccgcg cgccgttcac ctcgacgccg
    15301 atgccgggaa ccagcaccag gatgcagaga atcaccgcga ggccccaggc gaggtggtgg
    15361 caacgctgcc aaacggcgag cggcaccgcg agcacgacgc cgaacgcggt caggctgacg
    15421 agcaggaaga tgccgtgctt ccagaccagg ctttcactga tggcgacggc ggcggaggcc
    15481 accatgaccg tgcccagcac taggatgagc agccaggaca gcaggagcgt catgtcgagc
    15541 ctcgccgtca tgccgcgaac tccatgaccg ccgccgcgaa gcgctcaccg cgatcggcga
    15601 agttctcgaa catgtccagg ctggcgcagg cgggggaaag caggaccaga tcgccgggac
    15661 ccgccgcgtt gcgggccgcg accaccgcgg cctccagggt atccactctg acgagcggaa
    15721 cggcgccgtc cagagcccga gccatggcgt cggcatccac gccgagcaga acggccgtct
    15781 tcacgtagcg cgagaccggc tcccgcagag cggtgaaatc cgcacccttg ccgtccccgc
    15841 ccgcaatcag tatcagcgat cccccggagg actct
//
LOCUS       DBNHLM010000359         7512 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1133515, whole genome shotgun sequence.
ACCESSION   DBNHLM010000359 DBNHLM010000000
VERSION     DBNHLM010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7512)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7512)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7512
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1133515"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="AC16QF_16470"
     CDS             complement(<1..536)
                     /locus_tag="AC16QF_16470"
                     /inference="COORDINATES: protein motif:HMM:NF018827.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450206.1"
                     /translation="MSADTNTDTEPNRQPSASDTPGAGSDAFSDEIADRTEGIAAASA
                     FGVDTRAIMDAWLSIANEATTHPRAVLRAATKLGRELGSIVMGSSELAPAPEDKRFSD
                     AAWEANPIFRRIGQSYVAWTRSLDGWLDESGLEGIERERARFVLDVFKDVLAPVNSLA
                     GNPEALKKLRASDGGSLTR"
     gene            complement(785..1132)
                     /locus_tag="AC16QF_16475"
     CDS             complement(785..1132)
                     /locus_tag="AC16QF_16475"
                     /inference="COORDINATES: protein motif:HMM:NF020918.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HIR6450207.1"
                     /translation="MSMIEKQAKLGRELLELNVDTVRKFAELSSENFRRYMELNQSYA
                     QKLPEVREVGAFVELQRDYGQSLWDGVRDDFKARGDIVRTAVEHTGSLSRGAFGSEPE
                     VAEGQAEAAAEAA"
     gene            complement(1262..2773)
                     /locus_tag="AC16QF_16480"
     CDS             complement(1262..2773)
                     /locus_tag="AC16QF_16480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT/MGAT family O-acyltransferase"
                     /protein_id="HIR6450208.1"
                     /translation="MYKLGALDAGFLYNETERCPHHIASVQVLEPLPGTVPGEFVARL
                     KALLLERVHLVPYFTNKLQRAPFDLDHPVWVRDGDFDIDHHVRTIEVPAPGGREEFEA
                     TIAELHATPLDMSRPLWDLWVLTGLEGGRYAYYNRAHHACLDGMAGQKMIETIMDVTP
                     EPRKVTAPPLGFFAAQSETPTNLLVGALENFARHQVKQSSIWMNQLETTTRLIQRAMD
                     PSKGLGAAAEVAPRTRFNRTIERSRTYASGELSLADVKAVAKATGTKVNDVFLAVIGG
                     ALRRYLRRKGELPSKPLIAGCPVSLRQAGDESTNNQVTMMLVSCASDEADPIRRLLKV
                     AASSAQAKGLTADTAGSYDPDPALPGLPNVLAGMAQLIDRYNLADLDGVPLPCNLVVS
                     NVPGPVTQLYSNGAKMLTHYPVSLPVHGQGLNITVQSYLDEMFFAVTACAKALPDAGR
                     LRDDMLEEFAELREAMGPSDAMQHDVANERAPLPSRPQVVDGGSGAEIHDRAA"
     gene            3262..4062
                     /locus_tag="AC16QF_16485"
     CDS             3262..4062
                     /locus_tag="AC16QF_16485"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6450209.1"
                     /translation="MYEEVEVVEHRQKGPSVWDSATELPRILLEVTSLAYSWPLLTSA
                     PRGDGHPILVLPGFTAGDESTLVLRRFLSRLNYVPHAWEQGQNTGSFEVQERLVAHFY
                     RLTANLDRKISLIGQSLGGVYARALARQFPDRIRQVITLGSPFSSQGPEATNALVARL
                     FQYMSGMTAEEMRDQMLVLPEASLSVPTTAIYSKSDGVVHWSSCLELDGEQSENVEVL
                     GSHSGMAHNPLVFHVLADRLAQPEGEWRPFQRGKGCRSLLFPKPEARS"
     gene            4065..4511
                     /gene="phaR"
                     /locus_tag="AC16QF_16490"
     CDS             4065..4511
                     /gene="phaR"
                     /locus_tag="AC16QF_16490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HIR6450210.1"
                     /translation="MRELKKYPNRRLYDLDESHYVTVDDVRQMIVAGESIRVLDSKDG
                     SDITRSILLQILAEQEAEGHEPILTNRAIEQIIRFYGQNFGSVVSRYIEQSILTFLEH
                     QDQFRRRMREMSDLNPLNFVRQAMERTFGDTDRDGGRDGGRNKGDK"
     gene            complement(4522..4665)
                     /locus_tag="AC16QF_16495"
     CDS             complement(4522..4665)
                     /locus_tag="AC16QF_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450211.1"
                     /translation="MEAAFLMSQLPLLRLEAPRWRGPVLVLPGFMADVYRLISRILAR
                     PGS"
     gene            complement(4728..5936)
                     /locus_tag="AC16QF_16500"
     CDS             complement(4728..5936)
                     /locus_tag="AC16QF_16500"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6450212.1"
                     /translation="MSGTLLERVASLKSVIEEGGDEAQKLRRVPDETIRTLVDSGFFR
                     FTLPEELGGENATIRETIEVLEAISAIDGSVGWNVMLGSEINAMAAGGMDKELAKEVY
                     LDNPRVIMCGGGGPGSMPTKAVAQEDGSYRVWGQTTFISGCHNAEWCFMAAPIVENDA
                     PKMDENGMPMVRMWFLHRSEWEIVDTWDVAGLRGSGSHDVRAEGALVPERFVPVQLVS
                     LPAHYPNPVYRVPVPLRLAYNKAAVAIGVARGALDTFADLAQSKIPMLSSSTLRDRPI
                     AQYKMGECEAKLRAARAFLMEAMDAVHDELEAGAPTPSAERTYMGRLACTYAANASME
                     VVDVIHNAAGTSGMRMYSPLERKLRDAHGCATHRWVAHPLYQDLGKIFLGHEPAPEFA
                     GEGDPMQGKK"
     gene            complement(6110..6183)
                     /locus_tag="AC16QF_16505"
     tRNA            complement(6110..6183)
                     /locus_tag="AC16QF_16505"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6149..6151),aa:Cys,seq:gca)
     gene            complement(6254..6853)
                     /gene="pgsA"
                     /locus_tag="AC16QF_16510"
     CDS             complement(6254..6853)
                     /gene="pgsA"
                     /locus_tag="AC16QF_16510"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012315213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR6450213.1"
                     /translation="MNLPNLLTLGRILFVPVFVLVYVIPGERTYLLAATLFALAAFTD
                     WLDGYLARRLNQTTPFGAFLDPVADKLIVVTALVLLIGHHRTLWLTLPALVIVGREIV
                     ISALREWMAEMNRRGVVAVSWLGRVKTTFQMIAVVVLLANPPAFDRPWVLLGYGLLYV
                     AAAMTLASMALYLHAAWPTLKSGYGGPEGEPGEDPRDRS"
     gene            complement(6890..>7512)
                     /locus_tag="AC16QF_16515"
     CDS             complement(6890..>7512)
                     /locus_tag="AC16QF_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR6450214.1"
                     /translation="SHTMGEATVASCVVFDRNGPLKSDYRRFNIEDVAAGDDYAAMEQ
                     ALRRRYTRLKQGEGKLPDVLVIDGGRGQLQRAKDVLDELQVDGVHLLAIAKGPSRKPG
                     LEKLFANGAEVDYPEQGAAAHLLQHIRDEAHRFAITGHRQRRQRKRSRSELDDVAGVG
                     PKRRRELLTHFGGLSGVKGASVEEIAKVPGISRRLAEEIYGTLHAE"
BASE COUNT         1398 a         2453 c         2263 g         1398 t
ORIGIN      
        1 cgagtgaggc tacccccatc actggcgcgc agctttttca gcgcctcggg gtttccggcc
       61 aggctgttca ccggagccag cacgtccttg aagacgtcca gaacgaatcg cgcacgctcg
      121 cgttcgatgc cctcgagtcc cgactcatcg agccaaccgt ccagggatcg ggtccatgca
      181 acgtaggact gtccgatcct tcggaaaatg gggttggctt cccaagccgc atcactgaaa
      241 cgcttgtctt ccggcgccgg cgccagctcg ctggatccca tcacgatgct gcccagctcg
      301 cgacctagct tggttgcggc acgtagaacg gcgcggggat gagtggtcgc ttcgttcgcg
      361 atgctcagcc aggcatccat gatggccctg gtatccacgc caaacgcgct tgccgccgca
      421 atcccttccg tccgatcggc gatctcgtcg gagaacgcat cgctcccggc acccggggta
      481 tccgatgctg atggctgcct gttcggctcg gtgtctgtgt tggtgtcagc gctcacgcta
      541 gtcacctcgt ctgggtcgca tccatggtcc acatcttaga ccacctgatg atggttgaaa
      601 gaaaagcatc cggccatccc ggagattcgg acgtcaaccc ggcgggttga atcgacggcg
      661 acaggacggc atctcagccc ccaccaccga aaaaaaaagc cggctccgta acaacacaga
      721 gccggccttt tcatctgagc gccgggggaa cagcagatcc cgcggcatgc tcagtggaga
      781 tgcactacgc tgcttcggct gcggcttctg cctgaccttc ggctacctca ggctcggagc
      841 cgaacgcgcc gcgactcagg gagcccgtgt gctccacggc ggtgcgcacg atgtcgccac
      901 gcgccttgaa gtcgtcgcga acgccgtccc agaggctctg gccatagtcg cgctgcagtt
      961 ccacgaaagc gccgacctca cgaacttccg gaagcttctg cgcgtagctc tgattgagct
     1021 ccatgtacct gcggaagttc tccgaggaca actcagcgaa cttgcgaacg gtgtccacgt
     1081 tgagttccag gagctcgcgg ccaagcttgg cctgtttctc gatcatcgac atctcatttc
     1141 tcctcggcta ctagccatcg aatcggctaa tctgttgcga tgcacaacga attgtgcacc
     1201 acaatatatt gcattgcaac atttatttaa tttcaggagt acacgaccct cctgtacccg
     1261 atcacgcagc ccgatcatgg atttcggccc cggagccgcc atcgacaacc tgcggccggg
     1321 acggaagagg cgcgcgctcg ttggccacgt cgtgctgcat tgcgtcgctc gggcccatgg
     1381 cctcgcgcaa ctccgcaaat tcctcgagca tgtcgtcgcg caaacggccc gcgtcgggca
     1441 gcgctttcgc gcacgccgtc accgcgaaga acatctcatc cagatagctc tgcaccgtga
     1501 tgttgagccc ctggccgtgc acgggcaggg agaccggata gtgggtcagc atcttggcgc
     1561 cgttcgagta gagctgggtg acgggcccgg gaacattaga caccaccagg ttgcacggca
     1621 acggcacgcc gtcgaggtcg gcaaggttgt accgatcgat gagttgggcc atcccggcca
     1681 gcacgtttgg gaggcccggc aatgccgggt ccggatcgta gctgcccgca gtgtcagcgg
     1741 tgagcccttt cgcctgggcc gaggacgccg cgaccttgag gagtcggcgg atcggatcgg
     1801 cctcatcgga agcacagctg acgagcatca tggtgacctg attgttggtc gactcatcgc
     1861 ccgcctgccg caacgacacg gggcaaccgg cgatcagagg cttggacggg agttccccct
     1921 ttcggcgcag gtagcgccgc agagcgcccc caatgacggc caggaacacg tcgttgacct
     1981 tggtgcccgt ggcttttgcc acggccttga cgtccgccag ggacagctct cccgaagcat
     2041 aggtcctgga gcgctctatg gttcggttga agcgcgttcg cggtgccacc tccgcagccg
     2101 cccccaggcc cttgctcgga tccatggctc gctggatcag ccgcgtcgtc gtctccagct
     2161 gattcatcca gatggaagac tgctttacct gatgccgcgc gaagttttcc agcgcgccga
     2221 ccagcagatt ggtgggcgtc tcggactgag cggcgaagaa gcccagaggg ggcgccgtga
     2281 ccttacgggg ctccggcgtc acgtccatga tggtttcaat catcttctgc ccggccatcc
     2341 cgtcgaggca ggcgtggtgg gcgcggttgt agtaagcgta gcggcctccc tcgagaccgg
     2401 tcagcaccca caggtcccac agaggtcggc tcatgtccag cggcgtcgcg tgaagctcgg
     2461 caatcgtcgc ttcgaactcc tcccggcctc ccggagcggg gacctcgatt gtgcggacgt
     2521 gatgatcgat gtcgaaatcc ccatcacgca cccataccgg atgatcgaga tcgaacggcg
     2581 ccctttggag cttgttggtg aagtacggca caagatggac gcgctccagc aacagcgcct
     2641 tgagccgagc cacgaactcg ccgggaaccg ttcccggcag cggctcgagc acctgaaccg
     2701 aagcgatgtg atgcgggcat ctttcggttt cgttgtagag aaaacccgcg tctaaagcac
     2761 ccagcttgta cacttgaaac ctccatgatt ggtgtcacgt tgacgacggc cgccccgatt
     2821 tccggtaggc caacgagggc acggaatccg ccgcgtcgac tgacttctca gcaaaccccg
     2881 tgccatcagg caaaaatcgt ttaacccgtt gaatttaaat aacattattg tacgatccgc
     2941 tgtgcccgtc ctggtccacg ttggtgcgca gcgctgatcg cgcaccaaaa ccgcgccggt
     3001 tccgcgaacc gatggcgccg acatggcccc aagacatggc cccaaaatag tcgcaatatg
     3061 ttgcgttgca atatcaggaa aaagcgcgca gtttagcgtc agattgctac acgtcaatta
     3121 ccgagagcga aattatccgt cagagtgcct attttggggc ttcggtcaga ggcgaccgtg
     3181 tctattgcaa cgcagcataa acgtgttgac cgaggtaggt ttattcgtaa cactcgaatc
     3241 acctggaacg cagggctgcc cgtgtacgaa gaagtcgagg tcgtcgagca ccgtcagaag
     3301 ggcccatcgg tttgggattc cgccacggag ctgccgcgaa tccttctcga ggtcacttca
     3361 ctagcctaca gctggccact tctcaccagc gcgcctcgcg gggacggtca tcccatcctc
     3421 gtgctccccg ggtttacggc cggtgacgag tccaccctgg ttctacgccg ttttctcagc
     3481 cggttgaact acgttccgca tgcctgggaa cagggtcaga acacgggcag cttcgaagtg
     3541 caggaacgac tcgttgcgca cttttatcgg ctcacggcca acctggatcg caagatctct
     3601 ctcatcggcc agagtctggg cggcgtctac gcccgtgccc tggcccggca attcccggat
     3661 cgcattcgac aggtgatcac cctcggcagc cccttctcgt cccaggggcc ggaggccacg
     3721 aacgcgctgg tcgcgcgcct gttccagtac atgtcgggca tgacggccga agaaatgcgc
     3781 gatcagatgc tcgtgctccc cgaagcctct ctctcggtac ccaccaccgc catctacagc
     3841 aagtctgacg gcgtggtcca ctggtcgagc tgcctggagc tggacggcga gcagagcgag
     3901 aacgtcgagg tgctgggcag ccattcgggc atggcccaca acccgctcgt ctttcacgtc
     3961 cttgccgacc ggctcgccca gcccgaggga gaatggcgcc ccttccagcg cggcaaaggc
     4021 tgtcgatcgt tgctgttccc caagccggaa gcgcgttcct gaggatgcgc gagctcaaga
     4081 agtatcccaa ccggcgcctt tacgatctcg acgaaagtca ctacgtgacc gtggacgatg
     4141 ttcgtcagat gatcgtcgca ggcgaaagca tccgcgtgct cgacagcaag gacggctccg
     4201 acatcacccg atcgatcctt ctgcagatcc ttgcggaaca ggaggctgaa ggccacgagc
     4261 ccattctgac caaccgggcc atcgagcaga tcatccgttt ctacggacag aatttcggca
     4321 gcgtcgtgtc ccgatacatc gagcagagca ttctgacctt cctggagcac caggatcagt
     4381 ttcgccgcag gatgcgcgaa atgtccgacc tcaacccgct caatttcgtc cgccaggcca
     4441 tggaacgaac gtttggcgat accgatcgcg atggggggcg cgacgggggg cgcaacaagg
     4501 gagacaagta gcccctgccc ttcagctgcc gggcctcgcg aggattctgg aaatcaggcg
     4561 atagacgtcc gccatgaagc caggaaggac cagcacgggg ccacgccagc gaggcgcctc
     4621 gaggcgcagc aacggcagct ggctcatgag aaacgcggct tccacggccg cacgcccctc
     4681 cagcagctgg ttcagtcgtc ccggcggagc aatgaactcg cggtcggcta tttcttgccc
     4741 tgcatgggat cgccctcgcc cgcgaactcc ggcgccggct cgtgaccgag aaaaatcttg
     4801 cccaggtcct ggtatagcgg atgggctacc cagcgatggg tcgcacagcc gtgagcgtcc
     4861 ctcagcttgc gctccagagg gctgtacatg cgcattccgc tggtaccggc cgcgttgtga
     4921 atgacgtcca cgacttccat ggacgcgttc gcagcgtacg tgcaggcaag ccgtcccatg
     4981 taggtgcgtt ccgcggatgg ggtgggtgcc cccgcctcga gctcgtcatg caccgcgtcc
     5041 atggcctcca tgaggaaagc ccgggccgcg cgaagcttgg cctcacactc ccccatcttg
     5101 tactgggcaa tgggccggtc ccgcagggtg gaagacgaga gcatcggaat cttcgactgc
     5161 gccagatcgg cgaaggtatc cagagcgccc cgggcaacgc cgatggcgac cgctgccttg
     5221 ttgtaggcaa ggcgaagcgg caccggcacc cgatagacgg gattgggata gtgagcggga
     5281 agcgacacca gctgaacggg aacgaaccgc tccggaacga gcgcgccctc ggcccggacg
     5341 tcgtggcttc cggaccctcg cagcccggcg acgtcccagg tatccacgat ttcccattcg
     5401 cttcgatgga ggaaccacat gcgcaccatg ggcatgccgt tctcgtccat cttcggcgcg
     5461 tcgttctcga cgatcggcgc ggccatgaag caccactcgg cgttgtgaca gccgctgatg
     5521 aaggtggtct gtccccacac ccggtaactg ccgtcctcct gggccaccgc cttggtgggc
     5581 atgctgcccg gcccgccgcc gccgcacatg atcacccgag ggttgtccag atagacttcc
     5641 ttggccagct ccttgtccat acccccggcg gccatggcgt tgatctccga gcccagcatg
     5701 acgttccagc caacggagcc gtcaatggcc gagattgcct ccagcacctc gatggtctcg
     5761 cgaatggtcg cgttctcacc gccgagctct tcgggtagcg tgaaacggaa aaatccggaa
     5821 tccaccagcg tccggatcgt ctcatccggc acgcgccgca gtttctgggc ttcgtcgcca
     5881 ccctcttcga tgacgctctt caacgacgcg acccgttcca gcaacgttcc gctcacgggt
     5941 ggtatcagtt cttctgttgc aatctgctca gccacttctc gtctacctct caactctccg
     6001 cataccagga ttttacacga cccttcgcgg atgtctcacg ttcccgcggg cgcacgcccg
     6061 gcccccgccg cagcggcggg tggacgggtt tcgagtcccg tgggagattt ggaggctgag
     6121 gtcggaatcg aaccggcgta cacggagttg cagtccgctg catgaccact ctgccactca
     6181 gccttcgtcg ccggccggcg cgcgacgtca gtcgcggtcg ccctgcgaac acgccggcga
     6241 attctagcgg gctttaggag cgatcgcgag ggtcttcgcc cggctcgcct tccgggccgc
     6301 cgtaaccgga cttcagcgtc ggccaggcag catgcagata cagggccatg gaggcgaggg
     6361 tcatggcggc ggcaacgtaa aggagcccat agccgagcag cacccagggc cgatcgaaag
     6421 ccggcgggtt ggccagcagc acgaccaccg cgatcatctg aaaggtggtt ttcaccctcc
     6481 ccagccagga aaccgcaacg acgccgcggc ggttcatttc cgccatccac tcgcgcagcg
     6541 cgctgatgac gatctctcgc ccgacgatca ccagcgccgg gagcgtcaac cacagcgtgc
     6601 ggtggtggcc gatgagaagc acgagagcgg tgaccacgat gagcttgtcc gccaccgggt
     6661 cgagaaacgc cccgaagggc gtggtctggt tgaggcgtcg ggccaggtaa ccgtccagcc
     6721 agtcggtgaa tgcggccagc gcaaacagcg tggctgccag gaggtaagta cgttctcccg
     6781 ggatgacgta gacgagcacg aaaaccggca cgaaaagaat tcgccccagc gtgagcagat
     6841 tgggtaagtt gatgaccggt ttcatggctg tgtgctcacc gcgtaacgat cactccgcgt
     6901 gcagggtacc gtagatctct tccgccagcc tgcggctgat tccgggcacc ttggcgatct
     6961 cctcgacgct ggcacctttc acgccggaga gcccaccgaa gtgggtcaac agctcgcgcc
     7021 gccgcttagg ccccacgccg gcgacgtcat cgagctccga tcggctgcgt ttgcgctgac
     7081 ggcgttggcg gtggccggtg atggcaaagc ggtgcgcttc gtcgcgaatg tgctgaagca
     7141 gatgagcggc agccccctgc tctgggtagt ccacctccgc gccgttagca aacagctttt
     7201 ccagtcccgg ctttctgctg ggccctttgg cgatggccag caggtgcacg ccgtcgacct
     7261 gcaactcgtc gagaacgtcc ttagcccgct gcagttgccc gcggccaccg tcgatcacca
     7321 gcacgtcggg cagctttcct tcgccctgct tcaagcgagt gtatcgacgc cggagcgctt
     7381 gctccatagc tgcgtagtca tcgccggccg ccacatcctc gatgttgaat cgccgatagt
     7441 ccgacttgag cggcccgttg cggtcgaaga cgacgcagga cgcgaccgtg gcctcgccca
     7501 tggtgtggct ga
//
LOCUS       DBNHLM010000360         6122 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_415321, whole genome shotgun sequence.
ACCESSION   DBNHLM010000360 DBNHLM010000000
VERSION     DBNHLM010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6122)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_415321"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1928)
                     /gene="ftsH"
                     /locus_tag="AC16QF_16520"
     CDS             complement(<1..1928)
                     /gene="ftsH"
                     /locus_tag="AC16QF_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014819038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR6450215.1"
                     /translation="MMLAAWMLFLVAQFWRESSQVAAIPYSTFLEYQQAGRVADLVVG
                     TDQITGSIVAPEEGQPERFRTTRVDPELADRLAEDGIEFSGAVESTWLQRVLAWVLPM
                     ALVLAIWIFLQRRLGQSRGLGGGIMSVGKNKAKIYVEEDVKVSFEDVAGLAEAKEELK
                     EIIGFLRQPERYGRLGARLPKGILLVGPPGTGKTLLARAVAGEAGVAFFSISGSEFVE
                     MFVGVGAARVRDLFEQARGQAPCIIFIDELDAMGRARGMGPMAGGNDEKEQTLNQLLS
                     ELDGFDPSQGVVLLAATNRPEILDAALLRSGRFDRQILVDRPDKQARIEILRLHLRTL
                     SLAPSTEAEQIAALTPGFTGADLANLVNEAAILATRDDATHVGMEDFNRAVERIVAGL
                     EKKNRLLNPHERKVTAYHEMGHAMVARALPGSDPVHKVSIIPRGIGALGYTIQRPIED
                     RFLMSSGELRDKMTVLLGGWAAESIVFSEISTGASDDLARATDIARSMVTRYGMDDGL
                     GMVSLESERSTFLQMPGEYAATRRDFSEQTAREVDCAVRDLITQAFERAVAILEVHRG
                     AFTEGAERLLEKETLRGDELPTLITEPARPTPADREATPEVRRESNAARRAEADRRDI
                     ERADDEGMTVAGPRSLLP"
     gene            2322..2930
                     /locus_tag="AC16QF_16525"
     CDS             2322..2930
                     /locus_tag="AC16QF_16525"
                     /inference="COORDINATES: protein motif:HMM:NF014068.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HIR6450216.1"
                     /translation="MPYYAFLDAGLEVDIASPQGGRVPVEPNSMGWPLATDADRRFKR
                     DSTAMRQLAQSLRVGDLDPATYDVVFLVGGWGAAYDLGQSSELGEFMTQANANGAVIG
                     GVCHGPLGLLQATDSDGRPLVEGRRLTAVSDVQVQQLGIEITPLHPETELRELGALYE
                     KDTAFRDFFATHVVVDGNLVTGQNQNSGAETAHRMMEILAGR"
     gene            4192..5532
                     /gene="nhaA"
                     /locus_tag="AC16QF_16530"
     CDS             4192..5532
                     /gene="nhaA"
                     /locus_tag="AC16QF_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HIR6450217.1"
                     /translation="MVAGDRSGTDLPTVVVDRFTGPLARFLKVEAATGVLLLLAAVAA
                     LGLANSPWSVSFLAFWETPIGLHFGPFDFSRSLRHWINDGLMTFFFFVIALELKRALI
                     LGELRDLRVASLPFAGALGGMIVPASLYFVLMSGRPGANGWGTVMATDTAFVIGCLAL
                     FGSRIPASLRVFAVSVAVFDDVGAILIVAAVYGGALNLNALTLGMLGVVAVAVCARLG
                     MRSIPLYFLLGGAVWLCFDASGIHATMAGVVLGLMTPTRVWVSDTRLRGVLGRALSHP
                     EGEHWSGDSVERSDLLRAGTAVRESLSPLERLEMMLHPWVGFLIMPLFALANAGIAIS
                     GADIGQPVSVAIFAALVFGKPVGVLFFSGIAVRLGFAIRPLGLDWPLVAACALLTGIG
                     FTMSLFIAGLAYPPAMLNAAKVGVLTGSLVSALGGLLALLWLTFRRRAAQEAEL"
BASE COUNT          953 a         2013 c         2014 g         1142 t
ORIGIN      
        1 tggggcagaa gcgaccgggg tccggcgacg gtcattcctt catcgtcggc gcgttcgata
       61 tctcgacggt ccgcctccgc tcggcgggcc gcgttcgatt ccctccgcac ctctggcgtc
      121 gcttccctgt ctgccggcgt cggtctcgcc ggttcggtga tcaaggtcgg cagctcgtcg
      181 ccgcggagcg tctccttttc gagcagtcgc tccgcgcctt cggtgaacgc gccgcggtgg
      241 acctccagga tcgcgaccgc gcgctcgaac gcctgggtga tcagatcgcg cacggcacag
      301 tccacctccc gagcggtctg ttcggagaag tcgcgccgcg tggcggcata ctcgcccggc
      361 atctggagga aggtcgagcg ctcgctttcg agcgagacca tgccgaggcc gtcgtccatc
      421 ccgtaccgag tgaccatgct gcgagcgatg tcggtcgccc tggcaaggtc atccgacgcg
      481 cctgtggaga tctcgctgaa cacgatggac tcggcggccc acccgcccag cagcacggtc
      541 atcttgtccc tgagctctcc gctcgacatc aggaaccggt cctcgatggg acgctggatc
      601 gtgtagccga gagctccgat tccacgcggg atgatcgaca ccttgtgaac tggatcgctg
      661 ccgggaagcg cccgggccac catggcgtgt cccatctcgt ggtacgccgt gacctttcgc
      721 tcgtgcgggt tgagtagccg attcttcttc tcgaggccgg cgacgattcg ctccacggcg
      781 cggttgaagt cttccatacc gacgtgggtg gcgtcgtcgc gggtggccag gatggccgcc
      841 tcgttgacca ggttggccag gtcggccccg gtgaagccgg gcgtgagcgc ggcgatttgc
      901 tcggcctcgg tgctcggagc gaggctcagc gtccggaggt gcaacctgag gatctcgatg
      961 cgcgcctgct tgtcggggcg atcgacgagg atttggcgat cgaagcgccc cgagcgcagc
     1021 agcgccgcgt ccaggatctc gggtcggttg gtggccgcga gcagaacgac gccctggctg
     1081 ggatcgaagc cgtcgagctc ggaaaggagc tgattcaggg tctgctcctt ctcgtcgttg
     1141 ccaccggcca tcggccccat gccacgggca cggcccatcg cgtcgagctc gtcgatgaag
     1201 atgatgcagg gagcctgccc gcgcgcctgc tcgaacaggt cgcggacgcg agccgctccg
     1261 acacctacga acatctccac gaactcgctg ccgctgatgg agaagaacgc aacgccggcc
     1321 tccccggcga cggctcgagc cagcaacgtc ttccctgtgc ccggcggtcc caccagcagg
     1381 atccccttgg gcaggcgagc cccgagccga ccgtagcgct cgggttggcg caggaagccg
     1441 atgatctcct tcagctcctc cttcgcctcg gcgagtccgg ccacgtcctc gaagctgact
     1501 ttgacgtcct cttcgacgta gatcttcgcc ttgttcttgc ccacggacat gatgccgccg
     1561 ccgagccctc tgctctggcc gagtcggcgc tgtaggaaga tccagatcgc gagtaccagg
     1621 gccatgggca ggacccacgc gagcactcgc tggagccacg tgctctccac ggcgccgctg
     1681 aattcgatgc cgtcctccgc gagccggtcg gccagctcag gatcgacgcg cgtggtgcgg
     1741 aagcgctcgg gctgaccttc ttcgggcgct acgatgctcc cagttatctg gtcggtaccg
     1801 actacgaggt cggcgacgcg gccagcctgc tgatactcga ggaaggtgct gtatgggatg
     1861 gcggccacct ggctgctttc gcgccagaat tgggccacca gaaagagcat ccacgccgcc
     1921 agcatcatgt accagaggtt gaagcccttc gacgtaatgc ggcgggccag ccggctgccg
     1981 ggcgacgctc ctcgcttcgg gccttgcgac gcgacctcat cgcccgagga agcgtcctgc
     2041 gcaggccctg ggccgacgtc gccgggcgcc gagctcgacg tctccacggg agcccgagag
     2101 ccctcgtgcc tgcgccgtat cttcatgaag ctcctcggtc gctctgcaat caacccgcta
     2161 ggcccgccgc gatggatgac gcggtatcat tttttgaagt atccgcagag ggacttggat
     2221 caagctagtg gagcgcggcg gccggccgta tcgcacgaaa gagtgagtcg cctgtgctct
     2281 gcccgcatgg cggcggtact catgagccgc gggagttgtc cgtgccctac tacgccttcc
     2341 tggacgccgg gctcgaggtc gacatcgcca gccctcaagg gggtcgcgtc cccgtcgagc
     2401 cgaactcgat ggggtggcct ctggcgacgg acgcggatcg ccggttcaag cgggactcga
     2461 cggccatgcg ccagcttgcg cagtccttgc gcgtcggcga cctcgaccct gcgacgtacg
     2521 acgtcgtctt cctcgtggga ggctggggcg ccgcctacga tctaggacag agctcggagc
     2581 tgggtgagtt catgacccag gcgaacgcga acggcgccgt gatcggaggc gtctgccacg
     2641 ggccgctggg gctccttcag gccacggact cggatggacg gccactggtc gagggacgca
     2701 gactcaccgc cgtgagcgat gtccaggtcc agcagctcgg catcgagatc acgccgctgc
     2761 accccgagac ggagctacgc gagctcggcg cgctgtacga gaaggacacg gcttttcgcg
     2821 acttcttcgc gacgcacgtg gtggtcgacg gcaacctcgt gaccggccag aaccagaaca
     2881 gcggggccga gaccgcgcac cgtatgatgg agattcttgc agggcgatga ggcacgtcgc
     2941 ggccacgctc ctcgtcctga ctgctagggg aaatctccca caacgaccgt cgaacacccg
     3001 ccgatactgc ggtcgctcga cccgggtatc ttaaagatgc gcctgacgtg gcgccccagc
     3061 gatttgaggc ggattgcagc tgggagatca atctgctaaa cccgccgggg gtcccgagcg
     3121 gagggaatag cccgggaccc cgcagttttc ttaccccccg ttcgcgagcg atggtggggc
     3181 gagggctcag ccgatcaggc gggcgcttcg gacctgcggt atcgtgagct caggtgcccc
     3241 gacctgcgtc agaaccggcc ggggcactcc gtcacgtacc agacggagca gcgaatcggc
     3301 acacgttgct gttgagtccc gacggcgtcg tcacgaagtc ggcattcagg gtctctccga
     3361 cagcatcccc gtcgtaggcg caggagaggc acctataccc agggtggcgg tgacttcagc
     3421 tacactgcgg ctggaatcac ctaccggggc tcggtacacg ggtcgttcac gatcgcgatc
     3481 gacgagtcgg atctgacgtt cgcgacaacc gccgatccgg cccgatggcg cacgtacacg
     3541 agacccgggc gctgagtggg agtcgtcggg cggagtcttc tccttgcgtc gtgcggtcac
     3601 ctgcaacgga acctgcacca tcgcctacgg gcgccggcca tcgctgacga gcgcatcgta
     3661 cgagagaacc tggagtttcc ggcgtccctc ggcgctgacc atagccccga cgaatcctga
     3721 tgagcgtggg gtcggtagtt cacctggagc gggcagagcc cgcggagggg agcgagggcc
     3781 ggtatgacgg tcgcgaagcg ccggtcaggt aagcccgcag ctgaaatcta cccaggccca
     3841 ctgagggaag gggaagcccc gcttggtctt aggtggccgg gcggggtttc cccgttccta
     3901 ctcacgagga agtctccgtt gttctatccg ttcgcgcccg gaacgggacg ccagcgcctt
     3961 ccttcgcacg ccctgatctg ggtgctgata gaacttcaga atggtcgcgg gctccttcca
     4021 gccacccagt ggttcatgag gtcggaagcg aacttccgac aggctgaatg cctgctgagg
     4081 tctccaggtg tttcccgtcg atcatgtaga tcgtgaggcg accaccatcg gtcgcccgtg
     4141 gtgaaaatga gttcctggca ttgcgctata tttccgtatg ggatgcgaga cgtggtagcg
     4201 ggggatcgaa gcggaaccga cctccctacc gtcgtcgtgg accgattcac cggccccctc
     4261 gcgcgtttcc taaaggtgga ggccgcgacc ggcgttctgt tgctcctcgc cgcggtcgcc
     4321 gcactgggcc tggcgaactc accgtggtcc gtatcctttc tggcattttg ggagacgccc
     4381 atcggcctcc acttcggccc gttcgatttc agccgctcgt tgcggcactg gatcaatgac
     4441 gggttgatga cgttcttctt cttcgtcatc gctctggagc tcaagcgggc gctgattctc
     4501 ggcgagcttc gtgatctgcg cgtggcgtcg cttccgttcg ccggagcact cggaggcatg
     4561 atcgtaccgg cgagcctgta tttcgtgctc atgagcggcc ggcccggcgc gaacggctgg
     4621 ggcacggtga tggcgaccga caccgccttc gtcatcggct gcctggcgct cttcggctct
     4681 cgcatcccgg cgagcctgcg cgtgttcgcc gtgtcggtgg cggtgttcga tgacgtgggt
     4741 gccatcctga tcgtagccgc ggtctacggg ggtgccctga acttgaacgc tctgaccctc
     4801 gggatgctcg gagtcgtggc ggttgcggtc tgtgcccgcc tggggatgag gagtattccc
     4861 ctctacttcc tccttggagg cgcggtgtgg ctttgcttcg atgcttccgg catccatgcg
     4921 acgatggctg gcgtcgttct gggtctgatg acgccgacac gcgtatgggt gagcgacaca
     4981 cgtctccgag gcgtcctcgg ccgcgccctt tcccatccgg agggtgagca ctggagcggg
     5041 gactctgtgg agcgaagcga cctgctgcga gccggcactg cagtcaggga gtcgctcagc
     5101 ccgttggaac ggctggaaat gatgctccac ccatgggtcg gattcctgat catgccattg
     5161 ttcgctctgg ccaatgccgg catcgcgatc tccggtgccg acatcggaca gcccgtctcc
     5221 gtggcgatct tcgccgcact ggtcttcggc aagccggtcg gcgtcctgtt cttcagtggg
     5281 atcgccgtac gcctcggatt cgccatccgc ccgcttggct tggactggcc cttggtggcg
     5341 gcctgcgccc tcctgaccgg gatcggcttc accatgtcgc tgttcatcgc gggcctggcc
     5401 taccccccag cgatgctcaa cgctgccaaa gtcggcgttc tcacggggtc gctggtatcc
     5461 gccttgggcg gtctccttgc gctactctgg ctgacgtttc ggagacgggc cgcacaagaa
     5521 gcggaactct gaagcgccct ttgcgctgga gcggtcgctc acccacgacg cgggtaagta
     5581 cctgggcctc gaatgggcag gcaagcgttg cggcgggcct gaatagcgcg ccagcgaggg
     5641 cccgataccg tgttcgcgaa gcgcctgtcg gcgaggcccg cagctgaaat ctacccaggc
     5701 ccactgaagc tcccccgatc actgaccgag agcttcttca tgttcgtcgg ctaccgacct
     5761 cgcgaccgcg cagcgggctg tgtgccatat caggatcgcc cgccctgagt cggcaagagc
     5821 gcgacttgcc tgcgacgatg cagtgcatct agtctgcgcg tccgttcgca cggatgcagc
     5881 tgtgacacaa cctgcaccag cgcctaccgt cggtggctgt cgctgaccgg cgtgcagagt
     5941 gcgaaagccc aatgattcca gcgcccacca gcgcagaccc gcgccccggc ggattctgat
     6001 aagcgtgagg tcggtagttc aaatctaccc aggcccactg agcccgctcg gtcacgacgg
     6061 ccgggcgggc ttcttcatgc cataacgtcg agaactaccg acctcacggg tgcactccgg
     6121 tg
//
LOCUS       DBNHLM010000361        35675 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_355365, whole genome shotgun sequence.
ACCESSION   DBNHLM010000361 DBNHLM010000000
VERSION     DBNHLM010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 35675)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 35675)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35675
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_355365"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..333)
                     /locus_tag="AC16QF_16535"
     tRNA            complement(243..333)
                     /locus_tag="AC16QF_16535"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(296..298),aa:Ser,seq:cga)
     gene            complement(392..1183)
                     /locus_tag="AC16QF_16540"
     CDS             complement(392..1183)
                     /locus_tag="AC16QF_16540"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6450218.1"
                     /translation="MTAFASLGSGSRGNGTLVDLGGELILVDCGFTLKQAENRFRRLD
                     VRPGDLSAILVTHEHADHAGGVAALSHKYAVPVFASHGTFKATRNELVGRDIDSHASF
                     DVGAVTVMPVIVPHDAREPTQFVFQHGAVRIGVISDLGQITPFVIEQYQGCDGLMMES
                     NHDREMLLRGRYPESVKRRIAGNLGHLSNEQAASFLEQVGHADLRVAVGHVSEENNHP
                     ELLEETFARFSGQVAELTFATQEDGLGWIRLPEGGVSAAAALGAG"
     gene            complement(1180..2292)
                     /gene="bamC"
                     /locus_tag="AC16QF_16545"
     CDS             complement(1180..2292)
                     /gene="bamC"
                     /locus_tag="AC16QF_16545"
                     /inference="COORDINATES: protein motif:HMM:NF018509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HIR6450219.1"
                     /translation="MRVARALLVVPLVAGCSWLNDDEGIFVNKSDDYLDAREGPGLYV
                     PEDLATGRVSDPFPIPPSPEQMNPEFYPQRPPQPSAIYASDTRDEVRIQRLGDRRWLA
                     LPESPTTVWPKLKQFLAENGVPIALEMGPEGRIDTDWLLISEDTYRDVVRLVIRDGRD
                     IAELTTGRDRIRFRLEQGMRERTCEVHIRHENDSLGLPAADSLVDIEGVASDIADIEN
                     DMLNEFGAYVAAKVSEQTVSMVAQEISAGAKSVLTRNEQGDPTLILYLDYERAWAALG
                     QAIARAEMDVTDQNRDEGTYYVNITEAFLAGEEKKGFFGRMFSFGGGEDAKELQIQME
                     RSGEEIYSVSVLDENAAPVDRELGQQLLIMIREFAS"
     gene            complement(2310..3185)
                     /gene="dapA"
                     /locus_tag="AC16QF_16550"
     CDS             complement(2310..3185)
                     /gene="dapA"
                     /locus_tag="AC16QF_16550"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003086270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR6450220.1"
                     /translation="MFTGSIVALITPMTAGMEVDWTALERLIGWHLEEGTDGIVPVGT
                     TGESATLNIEEHLEVIRRTVSVVSGRVPVIAGTGANATAEAIHLTREAQKIGVDASLS
                     VTPYYNRPTQEGLYRHYAAIAEAVDLPIVLYNVPPRTGRDMTADTVARLAEIGNIVGI
                     KEACGDPERVAEIRGLVAEDFFVLSGEDGQTLRMFELGAVGCISVTANVLPRRMAEFC
                     RAWREGDVDLARAIDSELQPIHEILFVEPSPQPTKWALYEMGMVDRGIRLPMLELSEE
                     NRPELIRRLKAVGAL"
     gene            complement(3267..4370)
                     /locus_tag="AC16QF_16555"
     CDS             complement(3267..4370)
                     /locus_tag="AC16QF_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR6450221.1"
                     /translation="MRFLEVIGGWINRHFSNEEAIYLVVLIVIISTVVFIFGSMLAPV
                     LIGLIVAFLLQGLVKRLVDAGLGGGLAVYLTFIIFLGALIALVLFVVPIVWQQLRALV
                     DALPNGIVQVQALIRDLANTSPGGLVTPEQVDKWLGSLTGELGNLSRRLLNTLLTQVP
                     SVVALMIYTVIVPFSVFFFLKDRDELMRWFLSLLPEERPLLDRVGAEMNLQLANYVRG
                     KFVEILIVGVTTYVTFVLFGLNYAALLAVLVGLSVLIPFVGAAVVTIPVALVAVVQFG
                     WSLPLAGVLGAYLIIQFLDGNFLVPLLISSAVNLHPITIISAVLAFGGLWGIWGVLFA
                     IPLATLVKSIYNAWPRQEELAEASGDGLVEVDG"
     gene            complement(4370..4603)
                     /locus_tag="AC16QF_16560"
     CDS             complement(4370..4603)
                     /locus_tag="AC16QF_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR6450222.1"
                     /translation="MTEPVVVDAKGLNCPMPLLKAKKALNELSPGELVKVLATDPGSV
                     RDFEVFSRQSGHALLESLREDDTYIYLLRKKPG"
     gene            4732..6210
                     /locus_tag="AC16QF_16565"
     CDS             4732..6210
                     /locus_tag="AC16QF_16565"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR6450223.1"
                     /translation="MQDSRAKQRRLSGAGAVIRLGIGAALCLLTAAASTDVSDDLPVL
                     GDASSSMISPELERQIGRDFLKQVHAGLPTISDPVLKHFVTRQLTDLLQYSQVRENLL
                     GVVLIDSDQINAFAAPGGVVGVNLGLLLHAQDVHEYSSVMAHELAHLSQRHFARGVEE
                     ARSKTLPTIASLLAAIMIGAVGGGEAGMAAISASQAASQSSQLRYSRGREQEADRIGL
                     NTIVRAGMDPDGMARMFERMQRNYRFSRTPPEFLLTHPLSETRISDAKSQAREYPQRD
                     YADSPDYAMMRVRAQIYYAPNAAGAVAQFEKAVRDDPESVPARYGLALAQSRAGLHAE
                     AIAGGDALFTADPQKILYIASYAELLIEAEKLDQATRLLSHHLALNPDNATLAAMYSE
                     ALIRDQRYREAEAVLERQSVVNKDDVDVWYNLAEVSGKAGNIIGVHRARAEYFALHGA
                     YQRAIQHLEYARRLVREDNQQLHAMLDQRINDMRMALRMRRS"
     gene            complement(6207..7256)
                     /gene="nadA"
                     /locus_tag="AC16QF_16570"
     CDS             complement(6207..7256)
                     /gene="nadA"
                     /locus_tag="AC16QF_16570"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR6450224.1"
                     /translation="MSEATLPDYTLSADRVPVYEPEVLGPERVADLKSEIADLLAARG
                     AVLVAHYYVDGHVQDLAEETGGFVSDSLEMARFGHDHAAETLVVAGVRFMGETAKILS
                     PEKRVIMPTLEAECSLDIGCPPDDFEAFCKANPGRTVVVYANTSARVKAMSDWVVTSS
                     VALPIVEMLDARGEKILWAPDKHLGHYIQTQTGADMLLWSGACVVHEEFKSKALFDLK
                     AVYPEAGVLVHPESPAAVISIADVVGSTSQIIKAAQELPHDTFIVATDRGIFHKLRQR
                     NPDKRFIEAPTAGEGATCRSCAHCPWMAMNELSTLAAMLRSEGAMTANEIHVDPEIGR
                     RAMIPLKRMLDFKVP"
     gene            7368..7970
                     /locus_tag="AC16QF_16575"
     CDS             7368..7970
                     /locus_tag="AC16QF_16575"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6450225.1"
                     /translation="MYTLYEMGDSGNCYKIRLLMHQLDVPFERVPTDILQGRSRTESF
                     LALNPNGKVPLLVLPDGRPLAESNAILSYLARGTAYLPDEAYEQALVLQWLFFEQYSH
                     EPYIATNRFWIHILKDPVGHAQEIAAGHPKGEAALGVMETHLAAEEFFVAGRYTIADI
                     ALYAYTHLAHQGGYDLDPFPAVRAWLARVKAQPRHSPIDA"
     gene            7977..8843
                     /locus_tag="AC16QF_16580"
     CDS             7977..8843
                     /locus_tag="AC16QF_16580"
                     /inference="COORDINATES: protein motif:HMM:NF014951.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR6450226.1"
                     /translation="MPEILFLLGEAALARNDNHVRLPEAFAQAGWQVTALPHDCLGVH
                     AGRIHLDGHDPDRFDRIWPLGLGPRETFFDRMQMLAALDQRRLVIGADAFTYLHGKYR
                     WLDLMPETHAANDAEALARVIASGGDWVIKPPAGSYGQGVQVIREGEDPMPALRALTG
                     GEARRYCLVQRYVPEIVRGEKRTLVAGGEVCGTYLRTPTKDHRANLSAGGRASAAKLT
                     EEEARLVEGLTVELLSLGAAFAAIDIVAGRLMEVNVANPGGLATLENVYGRDPTPQAV
                     AALMAYWARTGG"
     gene            9055..9927
                     /locus_tag="AC16QF_16585"
     CDS             9055..9927
                     /locus_tag="AC16QF_16585"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6450227.1"
                     /translation="MGMLDGKVAVVTGAGGGIGRSHAKLLAKEGAAVVVNDLGGARDG
                     TGASQSMADTVVDEIIADGGRAIANYGSVSDKAAAQAMIDAAVSEFGRIDIMIANAGI
                     LRDKSFKNMDDEMWDVVLDVHLRGTYLTVKAAYDRMLEQGEGGRIIMTSSTSGLLGNF
                     GQTNYGAAKAGIAGFMRCLWLEGLKYGINVNVLVPTATSRLTEDILPEDIQDRFPPET
                     VSPAIVWLCTDEAKEVTGRQWLVAGNNVSLLSWQITPIADRDVSEGPWDVADIGQRIL
                     ASKESWPPINPLRG"
     gene            9938..11140
                     /locus_tag="AC16QF_16590"
     CDS             9938..11140
                     /locus_tag="AC16QF_16590"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6450228.1"
                     /translation="MSGPLEGFKVIDLSAVVSGPLTGALLADLGADVIKVERTVTGDI
                     QRNVGSRRNGFSGFFHVLNRGKRSIALNLTTREGCDIVHRLSADADVVLQNFRPGVVE
                     RLGIDYARLCADNPGLVYLSISGFGQSGPRAKDRAYDPIIQAYAGITDVQGSIHRDNP
                     DQPEQVNMLLLDKLTAWTGCQAIMAALLARSRTGKGQHVELSMLDTAISFIWSDVAAD
                     LILQGDDVDRRPPIRASGTMQTYADGHGFTMTLSQPEFEGLCRAYGLDHIAEDARFAS
                     LDLRMRHRAELREIISSEVDEVALELTVAQAEERLLRHEVPFARVRRLADLPEDEQIQ
                     HNQMFRELNHPVAGPLRDARPAPRFASTPAEPGGPAPSVGEHTRQILEEISMGAETEK
                     LFERGIVS"
     gene            11137..12240
                     /locus_tag="AC16QF_16595"
     CDS             11137..12240
                     /locus_tag="AC16QF_16595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6450229.1"
                     /translation="MNFGFTEEQNMLRDQVARFMQEACPIPKVREIGASQTGFDAALW
                     QQAAQLGWLSLIIPEENGGLGLRWIDLTVVLEETARGLCPLPIASQAMTAAAILRCGS
                     DSQKSSWLPAMAAGEVVCTTALYDDPNWIHPDAVQLTATPVEGGVRLDGVKPFVPDAL
                     AADYLLLAYRGPDGLGLAALSRDQVTFTPQPQMDSTKPAGRVVMDAVTVGDEFLLPLS
                     EADLDYLTDVGAVAVTAEMVGAAEAALNMTSEYAKERIQFGKPIGQYQGVKHRLADIY
                     VDVESFKSLLYYAAWAVDEAPDELPRAISMAKGYASDAFARIGIDGIGLHGAIGFTAE
                     YDIQLYLKRSKWARPMYGDSDYHLDRVATLGGL"
     gene            12240..13400
                     /locus_tag="AC16QF_16600"
     CDS             12240..13400
                     /locus_tag="AC16QF_16600"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6450230.1"
                     /translation="MDFEYTADEQAFREEVRDFLATNLPPREERGKDFLANWLTNVRE
                     KGWVGFSWPKEFGGSDGGLVEQAILREEMAVAKAPPLGTSFMGLAWVGPGIIQYGTED
                     QKRRFIPDILDSKVIWCTGYSEPNHGSDLAAIQCKAVREGDHYVVNGQKIWTSGAPWS
                     QWIILLVRTDFDVDVKHKGITCLLVPMDSPGVEVKPIENMAGDRHFAEVFFTDVKVPL
                     ENRLGDEGDGWKVTVSALANERSSIGEVTQMFGKLDTLKELVRTTTRQGRPASEDPKV
                     RRTIAMFETKIAAMKYNGLRYLTKQIKGEPLSSETSVNKLHRASLEIEMDDFAVELTG
                     QAGLLLSGSEEAVSGGSWAKSSLSWPNVVIGGGTPNIQRNIIAERILGQPKD"
     gene            complement(13427..14026)
                     /locus_tag="AC16QF_16605"
     CDS             complement(13427..14026)
                     /locus_tag="AC16QF_16605"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6450231.1"
                     /translation="MSVDVRIGLISDTHIPEARKTLWPQVFTAFDGVDMIMHAGDIHE
                     LYVLDALEEVAPVVAARGNGEDGGGGRPVQPDDPRVKYAWLLELGGLWVGLTHYVPVP
                     ERPPQFTLARWVERYFPERTPDIIVSGDTHREIIAEVQGVLCVNPGSPTYPHNYDTQY
                     GTLGFLDLVDGEARPSVWQITDGGIEPFDWDAVPPWKRR"
     gene            complement(14023..15291)
                     /locus_tag="AC16QF_16610"
     CDS             complement(14023..15291)
                     /locus_tag="AC16QF_16610"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6450232.1"
                     /translation="MSSRNIYQALEVPDFRWLWVGSLASSFAMNMQIVARGWLVYTLT
                     SSALDLAWVTLSFMVPMVAFSLWGGVVADRLPKRTIIAIAQALNTLATIVMGVIIFSD
                     LVSFWDFVWFGFFNGTVLALSMPARQAFAPELLPDRLIFTAMALNTTSWNLSRILGPA
                     FAGFLIAWVAAGDTSSTYGVGIVYVVIAALYFLSMATVLLVRKPGIVSSREQRKSPLT
                     DMADAFRYVWANPPVFGLIVLSIVPFLFGMPINTLLPAFNEDILGGGPDDLGLLMSTM
                     GMGAIVGSLLLAGVNDFRHRAAWLIATCVGWGAFTCAFGFSQTLMLSAGLLAVIGFLS
                     SWNMSLNRGLLQLQVDDHMRGRILSVDMMSHGLMPLGVIPISLIAEQFSVATALVVSG
                     AIFIVVVLLLLVVSPSIKRVDDDLQPVSGG"
     gene            complement(15394..16236)
                     /locus_tag="AC16QF_16615"
     CDS             complement(15394..16236)
                     /locus_tag="AC16QF_16615"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6450233.1"
                     /translation="MLKWQIGEVSVTQIVELSTASLGPYLLPQADPGALLEVPWLRPF
                     IDDDGRIVLSVHALVVESEGERLIVDTCIGNDKERTYPRWNRMQTDFLDRLAQAGYPP
                     ETIDTVMCTHMHVDHVGWNTRLTDDGWVPTFTRARYLFAEDEWAYWQREPQEFGPVVE
                     DSVQPIFDAGLAEVVGQTHRVTDEVWLEPTPGHTPGHVSVHIASAGEEAIITGDMIHH
                     PCQIAHPEWSSSADWNQEASAETRDAFLERYADRPVLVIGTHFAAPTAGHIVRDGESF
                     RLDY"
     gene            16377..17162
                     /locus_tag="AC16QF_16620"
     CDS             16377..17162
                     /locus_tag="AC16QF_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6450234.1"
                     /translation="MTRFGDIAVETTGHVAVLEIQRPPNNFFDVDLINAMGDAFEAMD
                     EDANVRALVLAADGKHFCAGANFGNRDEQSRRQARTATDGNPLYSAAVRLFACRKPVI
                     AAVQGAAVGGGFGLAVMPDFRVVCPETRFTANFVKLGFHPGFGLTYTLPRLIGQQRAN
                     LMFMTGRRIDGETACEWGLADILTDRDSLRARAMELAQEIAENAPLAVTSVRATLREG
                     VAEAVKRQTDHEFIEQHRLQQTEDHKEGVRAVAERRPGNFKGR"
     gene            complement(17149..18564)
                     /locus_tag="AC16QF_16625"
     CDS             complement(17149..18564)
                     /locus_tag="AC16QF_16625"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6450235.1"
                     /translation="MADGSPTPYADPRARVPNTAVVAYGAPGIGAGYMYLMMSLYVMK
                     FSTDVLLIAPAVMGVIFSVSRIWDAVSDPLAGYLSDRTRSRMGRRRTWILGSMIPIGG
                     AFIMVFAPPDGLSDGALVAWMAVAIIGFYSAMTGFLVPHLSLGAELSESYHERSRLFG
                     VRHGCFTFGSILSLVSFYFLISAEQQGAEEVRNTAFWLAVLAGGVMCLLMAFGVWKLR
                     ERADYQGRVNENPIQAFADVWRNRHARIIIAVSFIENVGSAAIGALTLYVAQYVVGAP
                     MWAPAIILCYMIPSTFSVPLWIPLSRRFGKIRLWIFSLVLTGVAFGSMFVLPFMESMP
                     LKLAYIATAAVFAGFAAGCGGTVSPSIQGDVIDYDEYLTGERKEGSYFAAWNFVYKSA
                     TGIMLLLTGFVLQASGFVPNQEQTMTVQLAMITLYSLFPLVCYSIGSLLLKRFDLDED
                     AHGEIRRALDERNAGRAASAP"
     gene            complement(18582..19358)
                     /locus_tag="AC16QF_16630"
     CDS             complement(18582..19358)
                     /locus_tag="AC16QF_16630"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6450236.1"
                     /translation="MKLSKLSRSVEGKVVIVTGAASGMGRATARVFADEGAKVAAMDI
                     NAEPLNEVVDEIAAAGRDIKGWALDLSDPDAIVEVFAEVPDHFGGIDILVNNAGISMF
                     APIDSDDYEHAWERSLTVLLTAHTRTIRAALPYLRKAEAARIINIASTEGLGATKFGS
                     PYTAAKTGVIGLTRSLAVELGEEGITVNCICPGPILTGMTAAIPDEAKAEFARRRVAL
                     KRYADPEEVAHATLSLALPAACFITGVALPVDGGLTIRNA"
     gene            19503..20843
                     /locus_tag="AC16QF_16635"
     CDS             19503..20843
                     /locus_tag="AC16QF_16635"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6450237.1"
                     /translation="MAEAYEFHDDDVIAPEVYGRHGHPHGAFAWLRANDPLRYVEPTE
                     YLPFWAVTKHADIVEIEKQPEIFASEPRPILVKEAQSPEVRQDVLMQIFERLKDSPKL
                     LEVLATIEQGGLIRSLVQMDPPDHPKYRTLVQPWFKPSNIKRLEDSLTRIIANILDEM
                     MGDGSERELEFVQDVAVWPPLKLITELLGVPEEDEWRILKLTNELFAGDDPEMKRAGD
                     DPLSIIDTIKDLYDYFTRLTDERRANPTEDLSSYIANGRIDGEYLPYKELISYYIIVA
                     TAGHETTRTAISGGLLALLENPEQMSLLRNNIDDEDLIKLAVEEMIRWTTPVSQFSRT
                     AMRDYELRGKQIRQGDALGLFYASANRDEEIFDDPFNFRVDRQPNRHLAFGTGPHICL
                     GILLARLEMRIFFKQFLPRIEKMELVDRGPEYLKASFVHGLKHLPIRFQLRPAR"
     gene            20863..21906
                     /locus_tag="AC16QF_16640"
     CDS             20863..21906
                     /locus_tag="AC16QF_16640"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6450238.1"
                     /translation="MSAVLDTLGERFAAYLTKSWKQPATTLHIEQIPGGASRETYRVR
                     VSIGGQEQGLILRRDPVSSLIDTERSLEYRTYEAVFPTSIPVPEPLLLEEDAVHLERP
                     FSVMREIGGCESSAQSLAAPPYQAMRETIGRRKWEILGQLASLDLSELGVDAFMAVPD
                     DPARRELEHWSKVIADDALHPQPIAEAARRWLERNLPEPSGRFALVHGDYRSGNFLYD
                     EEGVIRGVLDWEMAHIGDPLEDLAWSLDPLWSWPERELAGSLLPREQAIGIWEQASGI
                     TVDRDAFRWWQVFASYKGLAIWISSTEDFVNGASKEPILAVAGWLMSDRQNRILVDRL
                     NPRSDHAYAEPIV"
     gene            21903..22316
                     /locus_tag="AC16QF_16645"
     CDS             21903..22316
                     /locus_tag="AC16QF_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450239.1"
                     /translation="MIPNPSKALVDLAMKLSTGVLPEIGSPYAAANAGMTAVLLIMLS
                     QDSERAIANRMNDIDELKALFADAAAFPDRVGLASFVDAEPDSLLLADVDRLHADGMR
                     LLIDLHAWAESHDAGLDQEIWAFLGRHADRNRFDV"
     gene            22417..22492
                     /locus_tag="AC16QF_16650"
     tRNA            22417..22492
                     /locus_tag="AC16QF_16650"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22450..22452,aa:Lys,seq:ttt)
     gene            complement(22500..23354)
                     /gene="rfaP"
                     /locus_tag="AC16QF_16655"
     CDS             complement(22500..23354)
                     /gene="rfaP"
                     /locus_tag="AC16QF_16655"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011703.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HIR6450240.1"
                     /translation="MSGRERIELRSDLGQRWHGLDVFDVVDDLRGESVREMAGRSTIR
                     FELDGAGFYAKRHFGTGWREIFKNLLTLRRPVIDAGNEYRATRALSAAGVSTLEIAGF
                     GLRGRNPARRRSFLITDELAPVKSLEAYVLRWPEEPPAPGLKRRLIEEVAAISRAMHG
                     AGINHRDYYLCHLLMLHPELFGPQAPDPRIFVVDLHRAEIRNAVPYRWLVKDLGSLYF
                     SAYEIALTRGDRLRFLAAYFQMPWRDVLARHSGLIADIAKRADRLYRKGRRKGILPRQ
                     VAGLAPTR"
     gene            complement(23373..24887)
                     /locus_tag="AC16QF_16660"
     CDS             complement(23373..24887)
                     /locus_tag="AC16QF_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019485859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6450241.1"
                     /translation="MQQFDMVIVGAGVSGIGAACHFREKCPGKTFAILESRDAIGGTW
                     DLFRYPGIRSDSDMHTFGYNFKPWRNPKAIADGPSIREYVREAAREHDVDPHIRFNHR
                     LRSARWSSDSATWTLEVETPDGVVEFSCNFLFMCGGYYSYAGGYTPDFPGMADFEGPV
                     VHPQQWPDELDYSSKRVLIIGSGATAVTLLPAIAERAAHVTMLQRSPTYMVSRPDRDA
                     IANRLRRIFPEKVAYAITRWKNTTLQQFFYRRTRVNPEWVKDRLLRGVRAQLGPDFDV
                     QTHFTPSYNPWDQRLCLVPNGDFFRALRQGKATVVTDLIESFTSSGVRLKSGQTIEAD
                     VIVTATGLNLEVMSGVEFWVDEAKVNFPDTFTYKGMMFSGVPNLVQTFGYINASWTLR
                     ADLTSEYVCRLVNRMDELGVSQVAAQLREQDRDMPGKQWIDDFTPGYMQRSMHLFPKQ
                     GEKDPWRNTQNYAQDKKAIRHAPLEDGVLTFCNPDSAAPRLAGRASVARDQDAA"
     gene            complement(24998..25885)
                     /locus_tag="AC16QF_16665"
     CDS             complement(24998..25885)
                     /locus_tag="AC16QF_16665"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HIR6450242.1"
                     /translation="MPRIEDYLTPEQVEACLRRTDWQAARVLGVNYLMIVAAFALSIA
                     WPNPLTIVLSVLILGGRQLGMEVLVHDCAHSVFFARRRVNEIVGHWLCGGLVNTNLYR
                     YRDYHLGHHRYAGTDKDPDLYLAQMYPAPRDSMRRKLLRDVTGQTGIRDTWRQLRVMK
                     PGRNAPFLVLHGMMIAALAAVGALWAYLLWWAAYVFVYPLLTRVRYMGEHGVCFDYAS
                     ADPRENTSSTVASWWERLLVAPNRVNFHLEHHLLASVPLYHLPKMQRWLKESGFYGDK
                     DCFSHGYLDVVRKATVPAA"
     gene            25984..26787
                     /locus_tag="AC16QF_16670"
     CDS             25984..26787
                     /locus_tag="AC16QF_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450243.1"
                     /translation="MDDTNLTARQLILALLDSAPAPQIQAASLIAAGALFGMDPGAIR
                     VATARLVRDRVLESTARGQYTVGGGGAGLHRTVLSWADLEETLKPWAGEWLAVYLGSL
                     KRTNKTLVRARERALQLKGFVTVDPGLAVRPANLKASLPELLEDLRLLGLDPSAGLYR
                     IAETADGSRFAGLWDTDGLARRYQRHIDEIARSTRGIAELSPRAAAKETLLVGRAAIR
                     DILRDPLLPAELVDSRLRRELVHRMKKYDRLGKSCWRAFYADVGKADQR"
     gene            complement(26778..27446)
                     /gene="queC"
                     /locus_tag="AC16QF_16675"
     CDS             complement(26778..27446)
                     /gene="queC"
                     /locus_tag="AC16QF_16675"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HIR6450244.1"
                     /translation="MKRAIVLLSGGLDSATTLAVAKDEGFDCYALSIDYGQRHGAELA
                     AAREVARRAGVIEHRVIPVDVSVFGGSALTDRSIDVPEQPSEGIPITYVPARNTVFLS
                     LALAWAETLGLRDLFIGVNAVDYSGYPDCRPAFIFAFENLANLATKAGVEGDWYHVHA
                     PLIELSKAGIVELGISLGVDFGITVSCYQPDDDAAACGRCDSCRLRAQGFEEAGVEDP
                     TRYR"
     gene            complement(27443..28102)
                     /gene="queE"
                     /locus_tag="AC16QF_16680"
     CDS             complement(27443..28102)
                     /gene="queE"
                     /locus_tag="AC16QF_16680"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HIR6450245.1"
                     /translation="MTEQATRQLRISEIFLSLQGETSTVGLPTVFVRLTGCPLRCHYC
                     DTAYAFSGGEVMTLEDILARVRDLGVRRVTVTGGEPLAQPDCLGLLTSLCDEGFAVSL
                     ETSGALDVAGVDERVVKIIDVKTPGSGEVSRNRWENLAHCADGDQFKFVICHREDYEW
                     ARLKCEEHKLFEGACEVLFSPSSAELPARDLADWIVRDRLPVRMQIQLHKYLWGDQPG
                     R"
     gene            complement(28099..28857)
                     /gene="ybgF"
                     /locus_tag="AC16QF_16685"
     CDS             complement(28099..28857)
                     /gene="ybgF"
                     /locus_tag="AC16QF_16685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HIR6450246.1"
                     /translation="MTSPPKHRLRLVLKLALAGFLGSVVGPVAAEMTTETQSSAVPAP
                     AGDASEQRRLAELFYQLQVLRQEVQDLRGLVEEQSYQLNRLAKDQQEQYLDLDRRVAA
                     LRAQAPEPTLAETSPPPGGPVPGGVPGSERDAYTAAFNQMKNRQFDEAVASFNALVTD
                     YPNGQFTPNAFYWLGELHLAQADDEMARESFSQVINLYPDHQKAPDALYKLGIVHHRL
                     GDTANALQYLERVTAEYPQSSAAGLARTYSAEIR"
     gene            complement(28864..29406)
                     /gene="pal"
                     /locus_tag="AC16QF_16690"
     CDS             complement(28864..29406)
                     /gene="pal"
                     /locus_tag="AC16QF_16690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HIR6450247.1"
                     /translation="MTTRWFRTLLAIFFFGAVLAGCATGGQPAPAEPVAEPVETVDTT
                     PPPAPPQPTPPPAFDPNGNPFAPGTQRLLSRTFYFEYDRAVINPDDLASLELHASILR
                     ENPDRKIVIEGHCDERGTREYNLALGERRADAMRSFLTSAGVSRRQIETVSYGEERPE
                     DPGHDEGAWARNRRAIMIYR"
     gene            complement(29468..30781)
                     /gene="tolB"
                     /locus_tag="AC16QF_16695"
     CDS             complement(29468..30781)
                     /gene="tolB"
                     /locus_tag="AC16QF_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003086128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HIR6450248.1"
                     /translation="MSALVCLVLMLTSAATLAAESLDTIIIDAGYDKQTRIAVVPFRQ
                     GPEFSDRQQMGDIIGFDLVRSGQFSPLGMENMLSFPTTREEVFFRDWRVLGIEYLVIG
                     RTGVDAAGNLEVTYELFDVFNERRMTGGTTTALRSQWRDVAHRVADEVFEAITGIPGA
                     FSTRILYVLAQNVGSPDEAFRLEVADSDGERSRTLFSSTQPLLSASWSPDGTRVAYVS
                     FEGGRPSIVLQNVATGARERLTQFSGINGSPVFSPDGKRLALVLSRDGNPEIYIMDLD
                     DRRLRRITTHHAIDTEPSWTPDGKGVIFTSDRGGRPQIYQLDLATNFIERLTFQGDYN
                     ARARLLPDGKHLIFVHRRQGVFHIAWQDLERDDVRVLTQTSLDESPSLAPNGTMLIYA
                     TQDRGRGILAVVSIDGRVKYRLPSSSGDVREPAWSPYLDVLERVD"
     gene            complement(30799..31518)
                     /gene="tolA"
                     /locus_tag="AC16QF_16700"
     CDS             complement(30799..31518)
                     /gene="tolA"
                     /locus_tag="AC16QF_16700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HIR6450249.1"
                     /translation="MPAVRDYALPLLLALVLHALAFLSLRAGWNPETAEVRAIKPQIV
                     RSTLVMMEPKAKPVPRPKAAAKASPLPARQPEEPKPKAEARPADPPQAPPPDVGAERR
                     RAAQAERQERLEQLAEASFLDALETEAADLAESLAAEEAEAVAQTFRYGIYERVVANW
                     SRPPSARNNMQARLLVELVPTGDVVSVTLIEGSGNEAFDRSAEAAVRKARRFEVPKDS
                     DVFERYFRRFTLLFRPEDLLR"
     gene            complement(31525..31962)
                     /gene="tolR"
                     /locus_tag="AC16QF_16705"
     CDS             complement(31525..31962)
                     /gene="tolR"
                     /locus_tag="AC16QF_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HIR6450250.1"
                     /translation="MAKRRKPMSEINVVPYIDVMLVLLVIFMATAPLLTQGVIVDLPK
                     APSEPITDRQDDPLVVSIREDGAMFVNLGMQNADDESTRVTIYSLEDQAGKILRARPE
                     VPVYVKADHKLDYGAVVRVMTVLQRVGAQSVGLITDPPELAGE"
     gene            complement(31973..32656)
                     /gene="tolQ"
                     /locus_tag="AC16QF_16710"
     CDS             complement(31973..32656)
                     /gene="tolQ"
                     /locus_tag="AC16QF_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HIR6450251.1"
                     /translation="MPEQLSIFQLIANASLLVQVVMLLLALASVASWVMIFQRWFFLR
                     RVLASIADFEEHFWSGIDLRALYNELEAQDDLTGIESIFVAGFKEYTRLSEQSGADAD
                     SIMQGAQRAMRVALNREEENLETHLAFLATVGSTSPYVGLFGTGWGIMNSFRSLASMS
                     QATLASVAPGISEALVATAMGLFAAIPAVIGYNRFSARVEVMMSRYEAFSDELTGILH
                     RAAHARGGR"
     gene            complement(32668..33717)
                     /gene="ruvB"
                     /locus_tag="AC16QF_16715"
     CDS             complement(32668..33717)
                     /gene="ruvB"
                     /locus_tag="AC16QF_16715"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR6450252.1"
                     /translation="MSVEPDRLVSPREDGREGGFDRALRPERLDDYVGQCAVREQMEI
                     FIQAARARDEALDHTLIFGPPGLGKTTLANIIANEMGVAIKSTSGPVLERPAELAAML
                     TNLEEGDVLFVDEIHRLSPVVEEILYPALEDFQLDILIGEGPAARSLKLELPRFTLVG
                     ATTRAGLLTSPLRDRFGIVQRLEFYTVEELTQIVSRSAAKLGLEIHPEGATEVARRSR
                     GTPRIANRLLRRVRDWAEVKADGVINRKIADAALNMLKVDAQGFDSMDRRLLSAIIEK
                     FSGGPVGLDSLAAAISEERDTIEDVLEPFLIQSGYLMRTPRGRVATEQAYLHFGLSVP
                     EASEPSGGQPRLDLR"
     gene            complement(33714..34322)
                     /gene="ruvA"
                     /locus_tag="AC16QF_16720"
     CDS             complement(33714..34322)
                     /gene="ruvA"
                     /locus_tag="AC16QF_16720"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR6450253.1"
                     /translation="MIGRIQGQLVEVAENVILVNAGGICYEVELTSTALGRLPGADAP
                     VDLFTHFLVREDAQLLYGFASRDERDLFRALIRITGVGPKLALALISSIPLADLARAV
                     QTNDVALLTRVPGVGRKTAERLLVELKDRLADIVVVAEAGIPARGGKSAEEAEEVLVA
                     LGYKPQEAQRVVSDVLAEGSASTEEIVRAALRHIAMRKEVSG"
     gene            complement(34319..34792)
                     /gene="ruvC"
                     /locus_tag="AC16QF_16725"
     CDS             complement(34319..34792)
                     /gene="ruvC"
                     /locus_tag="AC16QF_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR6450254.1"
                     /translation="MALRILGIDPGSRITGYGLVEVSGGRIRYLASGCVRPRAKAFPE
                     RLAEIYTGIRSVISEHGPEVLAIEQVFMARNPDSALKLGQARGVAIAAAVQCGLPVHE
                     YAARTVKQAVVGSGRAGKEQVQHMIRAILDLPGAPAPDAADALAIAICHVNTSRP"
     gene            complement(34800..35540)
                     /locus_tag="AC16QF_16730"
     CDS             complement(34800..35540)
                     /locus_tag="AC16QF_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6450255.1"
                     /translation="MAGHSKWANIKHRKAAQDVKRGKLYTKIIREITVAARLGGGEVA
                     DNPRLRAAMDKALGANMPKDTIERAIARGAGGQDGSDVEELVYEGYGPAGVAILVEAM
                     TDNRNRTVAEVRHAFSKHGGNLGTDGSVAYLFNRQGVISFAPGADEEQIMEVVLEAGA
                     EDIETEGDGSVSVYTPWESLSEVVGALKAEQLEPDNAEVTMVASTYSACEPEDAEKVL
                     QLIDALEELDDVQNVYSNGDFPDSVLGA"
BASE COUNT         6612 a        11767 c        11303 g         5993 t
ORIGIN      
        1 cgacgttctg gtcaggccac gtcggggctg caatccccgg cgctggccgc tcttcccata
       61 agcaaggttg gctacaggta cacctatggg tacaccattt tcggaatctt ccattccgct
      121 gagtgtacct gcactctctt ccgttcgctt attctggtgt acccggaagc agactgcaag
      181 gcataacaaa tgggcgtttc agctaaatta tgagaaaaat cagccgaact tcagggggtt
      241 aatggcggag agggtgggat tcgaacccac ggaccccttg cgaggtcact tgatttcgag
      301 tcaagcccgt tcggccagct ccggcacctc tccgtgaggt cagatcagca agggcgacat
      361 tctgagcaaa ccgagggacg ttttccagcg attagccggc gcccaaggcg gcggccgcgc
      421 tgacgccgcc ctcgggcagg cggatccagc caagcccatc ctcctgagtg gcgaatgtca
      481 gctcggcaac ctggccgctg aaccgggcga aggtttcttc cagcagctcg gggtggttgt
      541 tttcctcgct gacgtggccg acggcaacgc gcaggtccgc atgacccacc tgctccagga
      601 acgacgctgc ctgctcgttg gatagatggc cgagattgcc ggcgatgcgg cgcttcacgc
      661 tttccggata cctgccgcgc aggagcattt cgcggtcgtg gttgctttcc atcatcaggc
      721 cgtcgcagcc ctgatactgc tcgatcacga aaggggtaat ctggccgagg tcggagatga
      781 cgccgatgcg gacggcaccg tgctggaaga caaactgcgt cggctcccgg gcgtcgtggg
      841 gtacgatcac cggcatcacg gtcacggcgc cgacgtcgaa gctggcatgg gaatcgatgt
      901 ccctgcccac gagctcattg cgggtggcct tgaaggttcc gtgggaagca aacacgggaa
      961 ccgcgtattt gtggctgagc gccgcgacac caccagcgtg gtcagcgtgc tcgtgggtga
     1021 ccaggatcgc cgagagatcg ccgggccgga catcgagacg gcgaaagcgg ttctcggcct
     1081 gcttcagggt gaatccgcag tccacgagga tcagctctcc acccagatcg acgagcgtac
     1141 cgttgccccg gctgccgctg cccagcgagg caaaagccgt caactggcaa actcgcggat
     1201 catgatcagc agctgttgcc ccagctcccg gtcgacggga gccgcgttct catcgaggac
     1261 ggaaaccgag tagatctcct ccccggaacg ctccatctga atctgaagtt ctttggcgtc
     1321 ttcgccaccg ccgaacgaga acatccggcc gaagaaccct tttttctctt ctccggccag
     1381 aaacgcttcg gtgatgttca cgtagtaggt gccctcgtcg cgattctggt cggtgacgtc
     1441 catctcggcc cgggcgatgg cctgacccag ggcggcccaa gcccgctcgt agtcgaggta
     1501 caggatgagg gtcggatctc cctgctcgtt acgggtgagc accgacttcg cgcccgctga
     1561 gatctcctgt gccaccatgg aaacggtctg ctcggatact ttggcagcga cgtaagcgcc
     1621 gaactcgttg agcatgtcgt tttcgatgtc cgcgatgtct gaggcgacgc cctcgatgtc
     1681 cacgagagag tctgcagcag gcaaccccag gctgtcgttc tcatgacgga tgtgaacctc
     1741 acaagttcgc tcacgcatcc cttgctcgag tcggaaccgg attcggtcgc ggcccgtggt
     1801 cagctcggca atgtcccggc cgtcgcggat gacaaggcga accacgtcac gataagtgtc
     1861 ctcgctgatg agcagccagt ccgtgtcgat gcgcccttcc ggacccatct ccagcgcgat
     1921 gggcacgccg ttctccgcca ggaactgctt gagcttcggc cataccgtgg tgggcgactc
     1981 gggcagcgcc agccagcgcc tgtctcccaa ccgctgaatg cggacttcgt cacgggtgtc
     2041 gctggcgtaa atggcgctgg gctgcggcgg gcgctgcggg tagaactccg gattcatctg
     2101 ctcgggagac ggcgggatgg gaaaggggtc ggacacccgc ccggtggcca gatcctcggg
     2161 aacataaagt ccggggccct cccgggcatc aaggtagtca tcgctcttgt tgacgaagat
     2221 gccctcgtcg tcgttgagcc acgagcatcc cgccacgagc ggcacgacca gcagcgctct
     2281 cgcgactctc atcaggcagt gcgcgcgcat cacagagccc caaccgcctt caggcgccgg
     2341 atcagctccg gacggttttc ctctgaaagt tccagcatag gcagacgtat gccgcgatcc
     2401 accataccca tctcgtagag cgcccacttc gtcggttgag gactcggctc cacgaacagg
     2461 atctcgtgaa tcggctgcaa ctccgaatcg atggcccgcg caaggtccac gtccccctca
     2521 cgccaggcac ggcagaactc ggccattctg cgcggcagca cgttggcggt caccgagatg
     2581 caacccaccg cgccgagttc gaacatcctc agcgtctgcc catcctctcc agacagaacg
     2641 aagaagtcct cagcgacgag accgcggatc tcggcaaccc gctccgggtc gccgcacgct
     2701 tccttgatgc ctacgatgtt gccgatttcg gcaagacgcg ccaccgtatc ggccgtcatg
     2761 tcgcgaccgg tgcgtggcgg aacgttatag agcacgatgg gaagatccac cgcttcggca
     2821 atcgctgcgt agtgacgata aagaccttcc tgggtcgggc ggttgtagta gggcgtgacc
     2881 gacaggctcg cgtccacgcc gatcttctgt gcttcccgtg tcaggtgaat ggcctctgcc
     2941 gtcgcgttgg cgccggttcc cgcgatcacc ggtacccgtc cggacaccac cgacaccgtc
     3001 cgccggatga cctcgaggtg ctcctcgata ttgagcgtgg cagactcgcc cgtggttccc
     3061 acggggacga taccgtccgt gccttcctcc agatgccagc cgatcagacg ctccagggcg
     3121 gtccagtcga cctccatccc cgcggtcatg ggcgtgatca gggcaacgat gctacctgtg
     3181 aacatatcgc tctctagctg ggcgacccgg ccctagggac gcggggagca gaaccgcgcc
     3241 atggtacttg agcggcgcaa cggggctcaa ccatcgactt cgaccaggcc gtccccgctg
     3301 gcttccgcca actcctcctg gcgcggccag gcgttgtaga tggacttcac cagcgtcgcc
     3361 agaggaatgg caaaaagaac cccccatatc ccccacaggc cgccgaaagc gagaacggca
     3421 gagatgatgg tgatggggtg caggttgacg gcgctggaaa tcagcagcgg gaccagaaaa
     3481 ttgccgtcca ggaactgaat gatcaggtac gcgcccagca cgcccgccag gggcagggac
     3541 catccgaact gcacgacggc caccagcgcg acggggatcg tcaccacggc cgccccgaca
     3601 aacggaatca gcaccgagag ccccacgagc accgctagga gcgcggcgta attgagaccg
     3661 aacagcacga aggtcacgta ggtggtcacg ccaacgatga ggatctcgac aaacttgccg
     3721 cgaacgtagt tggccagctg aaggttcatc tccgcgccga cccggtccag cagcgggcgc
     3781 tcctcgggga gcagagagag gaaccagcgc atgagctcgt cacgatcctt caggaagaag
     3841 aacaccgaga aaggcacgat caccgtgtag atcatcagcg ccaccacgga cgggacctgg
     3901 gtgaggagcg tgtttagcag cctgcggctc aggttgccca actctcccgt cagcgagccc
     3961 agccacttgt ctacctgctc aggcgtcacc agcccacccg gcgaggtgtt ggccagatca
     4021 cggatgaggg cctgcacctg cacgatcccg ttcggaagcg cgtcgaccag ggcacgaagc
     4081 tgctgccaga cgatgggcac cacgaagaga acgagggcga tcagggcgcc aaggaagatg
     4141 atgaacgtca ggtaaacggc taacccgccg ccgagccccg cgtccaccag acgtttcacc
     4201 aaaccctgga ggagaaaggc gacgatgagc ccgatcagca ccggggccag catcgacccg
     4261 aagatgaaca ccacggtaga gatgatgacg atgaggacca ccaggtagat ggcttcctca
     4321 ttggagaagt gccggttgat ccagccgcct atgacctcta gaaagcgcat cagcccggct
     4381 tctttcgcag caggtagatg taggtatcgt cctcgcgaag cgattccagc agagcgtggc
     4441 cgctctgccg cgaaaacacc tcgaagtcgc gaaccgatcc gggatcggtg gcgaggacct
     4501 tcacaagctc gcccggggag agctcgttga gagctttctt ggctttaagg agcggcatcg
     4561 ggcagttcag gcccttggca tcgaccacta cgggttcggt catgttcggt tcagcgcacg
     4621 gatgagcggc agcaggatgc ctatcataag acttttgaac ttttcccgcc cgcaggtggc
     4681 taaaaccaga gcaggttctg cggcacaccg ccatccagta tgatctccag aatgcaagac
     4741 tcccgagcga agcagcgacg tctgagtggc gccggggccg tgattcgcct cggcatcggc
     4801 gctgcgttgt gcctgctcac ggccgcagcc tccacggacg tcagcgacga cctgcccgtg
     4861 ctcggcgacg cctcctcgag catgatctcc ccggaactcg aacgccagat cggcagggat
     4921 ttcctcaagc aggtgcacgc gggcctgccg accatcagcg accccgttct caaacacttc
     4981 gtcacgcgcc agctgaccga cctgcttcaa tactcccagg tcagggaaaa cttgctgggc
     5041 gtggtgctca tcgacagcga tcagatcaac gcctttgccg cccccggcgg cgtggtgggc
     5101 gtcaacctgg gacttctgct gcatgcccag gacgtgcacg aatactcctc ggtcatggca
     5161 cacgagcttg ctcacctcag ccagcgccac ttcgccaggg gcgtcgagga ggcccgcagc
     5221 aaaaccctgc ccaccatcgc ctccctgctg gccgccatca tgatcggcgc cgtgggcggg
     5281 ggcgaagcgg gcatggcggc catatctgct tcccaggcgg ccagccagtc cagccagctg
     5341 cgctacagcc ggggccgcga gcaggaagcc gaccggatcg gcctcaacac catcgtccgg
     5401 gccggcatgg atccggacgg catggcgcga atgtttgaga ggatgcagcg caactaccgc
     5461 ttttcccgca cgccgccgga gttcctgctg acccacccgt tgagcgagac ccgcatctcg
     5521 gacgcgaaaa gccaggcccg cgagtacccc caacgcgact acgccgactc acccgactac
     5581 gccatgatgc gggtccgcgc ccagatctac tacgcgccca acgccgccgg ggccgtggcc
     5641 cagttcgaga aggctgtgcg ggacgatccc gagtcggtcc ccgcgcgcta tgggctggcc
     5701 ctggcgcaga gcagagcggg tctgcacgcc gaggccatcg ccggcggtga tgctctgttc
     5761 accgccgacc ctcagaaaat tctctacatc gcgtcatacg cagagctgct catcgaggcg
     5821 gagaaactcg atcaggcgac ccgtctgctg tcgcatcacc tggccctcaa cccggacaac
     5881 gccaccctgg cggccatgta cagcgaggcg ctcatcaggg accagcggta ccgggaggct
     5941 gaggcggtgc tggagcggca aagcgtcgtc aacaaggacg acgtggacgt gtggtacaac
     6001 ctcgccgagg tatcaggcaa agcgggcaac atcatcggcg tgcaccgggc tcgcgccgag
     6061 tacttcgcgc tgcacggcgc ctaccagcgg gccatccagc atctggaata cgcgcgccgc
     6121 ctggtgcggg aagacaacca gcagcttcac gccatgctgg atcagcgcat caacgacatg
     6181 cggatggcgc tgcggatgcg gcggtcctag ggcaccttga agtcgagcat cctcttgagc
     6241 gggatcatcg cccgacgacc gatctcaggg tccacatgaa tctcgttggc cgtcatcgcc
     6301 ccttcgctgc gcagcatggc cgccagggtg gataactcat tcatcgccat ccaagggcag
     6361 tgggcacagc tgcggcaggt tgcgccctcg ccggccgtgg gcgcttctat gaatcgcttg
     6421 tccgggttgc gctggcgaag cttgtggaag atgccgcggt cggtggccac gatgaaggtg
     6481 tcgtggggca gctcctgggc cgccttgatg atctggctgg tcgagcccac cacgtcggct
     6541 attgaaatca ccgcggccgg tgactcgggg tgcacgagga cgcccgcctc cggataaacg
     6601 gcctttaggt cgaagagcgc cttcgacttg aactcttcgt ggaccacaca ggcgccgctc
     6661 cacagcagca tgtcggcgcc ggtctgagtc tgaatgtaat gtcccagatg cttgtcgggg
     6721 gcccagagta tcttttcacc tcgggcgtcg agcatctcca cgatgggcag ggccacgctg
     6781 ctggtcacca cccagtcgga catcgctttg acccgggccg aggtattggc gtagacgacc
     6841 accgtccggc caggattggc tttgcagaag gcctcgaagt cgtccggcgg gcagccgatg
     6901 tccagagagc actcggcctc gagcgtcggc atgatcaccc gcttctctgg cgagagaatc
     6961 ttggcggtct cgcccatgaa acgcacgccc gcgaccacca gcgtctcagc ggcgtggtcg
     7021 tgcccgaatc gggccatttc cagcgagtcg gagacgaacc cgcccgtttc ctctgcgagg
     7081 tcctgaacgt gaccgtccac gtagtaatgg gccaccagca cggcgccgcg ggcggcgagc
     7141 agatcggcaa tctcggattt cagatctgca acccgttccg gcccgagcac ctccggctcg
     7201 tagacgggta cccggtccgc ggagagggtg tagtctggca gcgtagcttc gctcatgaca
     7261 tcgagaatat tggaagcgca gggaaaatca cagttcccgt gcaaccgtcg cctgtttata
     7321 tgaccatctt tttattgggg cgaatcgacg aaaaccaagg aggttcgatg tacacgcttt
     7381 acgagatggg agactcggga aactgttata agatccggct attgatgcac cagctggatg
     7441 ttcctttcga gcgagtaccc accgacatcc tccaggggcg gtcgcgcacg gaatcgttcc
     7501 tggcgctgaa tcccaatggc aaggtcccgc tgctcgttct acctgacggg cgcccgctgg
     7561 cggagtcaaa cgccattctg agctaccttg cgcgaggtac ggcctacctc ccggacgagg
     7621 cttacgagca ggcgctggtg ctgcaatggc tcttcttcga gcagtacagc catgagccct
     7681 atatcgccac caaccgattc tggattcaca tactcaagga ccccgtcggc cacgcacagg
     7741 agattgcggc cggacatccc aagggcgagg cggcccttgg agtgatggaa acccatctcg
     7801 ccgcggagga gttcttcgtc gccggccgct acaccatcgc ggacatcgcc ctgtatgcct
     7861 acacccacct cgcccaccag ggtggctacg acctggatcc ctttccggcg gttagggcct
     7921 ggcttgcgcg ggtcaaggca cagccgcgcc acagtcccat cgacgcctga ccgcccgtgc
     7981 cggagattct ctttctgctg ggagaggcgg cactcgcccg caacgacaac cacgtgcggt
     8041 tgcccgaagc gttcgcccag gcgggctggc aggtcaccgc cctgccccat gactgcctgg
     8101 gcgttcacgc cggcaggatc cacctcgatg ggcacgaccc cgaccgcttc gaccggatat
     8161 ggccgctcgg gctcggcccc agggagacgt tcttcgaccg catgcagatg ctcgcggcgc
     8221 tggaccagcg acgtctggtg atcggcgccg atgctttcac ctaccttcac ggcaagtacc
     8281 ggtggctgga cctcatgccc gagacccatg ctgcaaacga cgccgaggcc ctggcacgag
     8341 taatcgccag cggcggcgac tgggttatca agccccccgc cgggagctac ggtcaggggg
     8401 tgcaggtgat ccgggaaggt gaagacccca tgcccgcgct gcgcgccctc acgggcggcg
     8461 aggcgcgacg ttactgcctg gtccagcgct acgtccccga gatcgtccgg ggcgagaagc
     8521 gcaccctggt agcgggcggg gaagtctgcg gcacctacct gcgcacaccc acgaaagacc
     8581 atcgtgccaa cctgagcgcg ggcggtcggg cgagcgctgc caagttgacg gaggaggaag
     8641 ccaggctcgt cgaggggctg acggtcgagc tgctgagcct cggcgccgcg ttcgcagcca
     8701 tcgacatcgt ggccggccgg ctgatggaag ttaacgtggc caatccgggc gggttggcca
     8761 ccctcgagaa cgtctacgga cgcgacccga cgccccaagc cgtggccgcg ctcatggcct
     8821 actgggcccg aaccggcggc tgagcccggc gcttgcgttt gcggcgcttc gccttagact
     8881 gcaccgttcg tccgattcgg cgggcgaagg actgggggtt tgaacgttcc gctcagcctg
     8941 cggcaggcaa cgccgctcgg gcgcgtcaac cgttgcagca cgcactgcag caagcacctc
     9001 ccctacgctt cctgccgacc ggactgctga atcgcacaga acaaggagat cgatatggga
     9061 atgctcgacg gcaaagttgc tgtggtgacc ggcgcaggcg gcggtatcgg acgatcccac
     9121 gccaagctgc tggccaagga aggggccgcc gtggtggtca acgacctggg cggcgcccgc
     9181 gacggcacgg gagcatccca gtccatggcc gacaccgtcg tggacgagat catcgcagac
     9241 ggcggccggg cgatcgcgaa ctacggctcc gtctccgaca aggccgcagc ccaggccatg
     9301 atcgatgccg ccgtcagcga gttcgggcgc atcgacatca tgatcgccaa cgccggcatt
     9361 ctccgcgaca agtctttcaa gaacatggac gacgagatgt gggacgtggt cctcgacgtt
     9421 catcttcgtg gcacctacct caccgtcaaa gccgcctatg accggatgct cgaacaggga
     9481 gagggcggcc ggatcatcat gaccagctcc acctcggggc tgctgggcaa cttcggccag
     9541 accaactacg gcgcggccaa agccggcatt gcgggattca tgcgctgcct ctggctcgag
     9601 ggcctgaagt acggcatcaa cgtcaacgtg cttgtaccca ccgcaacgtc gcggctcacc
     9661 gaagacatcc tccccgagga catccaggac cgattcccgc cggagaccgt atcgccggcc
     9721 atcgtctggc tgtgcacgga cgaggccaag gaggtcaccg gccggcagtg gctggtcgcc
     9781 ggcaacaacg tctcgctgct ctcctggcag atcaccccca tcgccgaccg ggacgtctcg
     9841 gaagggccgt gggacgtggc ggacatcggg cagaggatcc tggcgagcaa ggagagctgg
     9901 ccgccgatca atcctctccg gggctgaggc aggctccatg tccggtcccc tcgaaggctt
     9961 caaggtcatc gatctgtccg cggtggtctc cgggccgctg accggagccc tgctcgccga
    10021 cctgggtgcc gatgtcatca aggtcgagcg gacggtcacc ggcgacatcc agcgcaacgt
    10081 gggcagtcgc cggaacggtt tttccggatt ctttcacgtg ctcaaccgag gcaagcggtc
    10141 catcgcgctg aatctcacca cccgggaggg ctgcgacatc gtccaccgcc tctccgcgga
    10201 cgccgacgtg gttctgcaga acttcaggcc cggtgtggtg gagcgtctgg gcatcgatta
    10261 cgcgaggctt tgtgctgaca atccggggct cgtctacctt tccatcagcg ggttcggcca
    10321 gtccgggccc cgggccaaag atcgggccta cgaccccatt atccaggcct acgccggcat
    10381 caccgacgtg caggggagca ttcatcggga caatcccgac cagcccgagc aggtgaacat
    10441 gctgctgctg gacaagctga cggcctggac cgggtgccag gcgatcatgg ccgctctgct
    10501 ggcacgcagc cgcacgggca agggccagca cgtggagctg tccatgctgg acaccgccat
    10561 ctcgttcatc tggtccgacg tggccgccga cctgattctg cagggggacg acgtcgacag
    10621 gcgcccgccc atccgcgctt ccggcaccat gcagacctac gccgacggcc acggcttcac
    10681 gatgacgctg agccagcctg agttcgaggg cctgtgccgt gcctacggcc tggaccacat
    10741 cgccgaggac gcccgcttcg ccagcctgga cctgcgcatg cggcaccgcg ccgagctgcg
    10801 cgagatcatc agctccgagg tcgacgaggt ggcgctggag ctcacggtgg cgcaggcgga
    10861 ggagaggctg ctgcgtcacg aggttccttt cgcccgggtg cggcgcctgg ccgacctgcc
    10921 cgaagacgag cagatccagc acaaccagat gttccgggag ctgaatcacc ccgtggcggg
    10981 accattgcgc gacgcgaggc ccgccccgcg attcgccagc accccggcgg agcccggcgg
    11041 gcccgcgccg agcgtgggag agcacacgcg acagattctg gaggaaatca gcatgggcgc
    11101 cgagaccgag aaactgttcg agcgaggtat cgtttcatga attttggctt caccgaggag
    11161 cagaacatgc tgcgcgacca ggtcgcgcgc ttcatgcagg aggcctgccc cattcccaag
    11221 gtgcgcgaga tcggtgcttc acagaccggc ttcgatgcgg ctctttggca acaggcggcg
    11281 cagctcggct ggctgagcct catcatcccc gaggagaacg gcggccttgg actgcgctgg
    11341 atcgatctga ccgtggtgct ggaggaaacg gcccggggac tttgcccgct gcccatcgcc
    11401 tcccaggcaa tgaccgccgc cgccatcctc agatgtggca gcgactcgca gaaatcgagc
    11461 tggctgccag ccatggcggc cggggaagtc gtttgcacca cggcgctgta cgatgatccg
    11521 aactggattc atcccgacgc cgtgcagctc accgcaacgc ccgtggaggg cggcgtgcgc
    11581 ctggacggcg tcaagccttt cgtgcccgac gctctcgccg ccgactacct gctgctggcc
    11641 taccgagggc ccgacgggct ggggctggca gcactcagcc gcgaccaggt cacctttacc
    11701 ccgcagccgc agatggacag caccaagccg gccggacgtg tggtcatgga cgcggtcacc
    11761 gtcggcgatg agtttctgct gccgctgagc gaggcggatc tcgactacct cacggacgtc
    11821 ggcgccgtcg ccgtgacggc ggagatggtg ggtgccgccg aggccgccct gaacatgacc
    11881 agcgagtacg ccaaggagcg catccagttc ggcaagccaa tcgggcagta tcagggcgtc
    11941 aagcaccgac tggcggacat atacgtcgac gtcgagtcgt tcaagtcgct gctgtactac
    12001 gccgcctggg ccgtcgacga agcgcccgac gagttgcccc gggccatctc catggcaaag
    12061 ggttacgcct ccgacgcctt tgccagaatc ggcattgacg gcatcggcct gcacggcgcc
    12121 atcggcttca ccgccgagta cgacattcag ctttacctca aacggtcgaa gtgggcgcgc
    12181 cccatgtacg gggactccga ctatcacctg gatcgggtcg caaccctggg aggtttgtag
    12241 tggatttcga gtacacggcc gacgagcagg cgttcaggga agaggttcgc gattttctgg
    12301 cgaccaacct gccgccccgg gaagaacgcg gcaaggactt cctcgccaac tggctgacga
    12361 acgtcagaga gaagggatgg gtcgggtttt cctggccgaa ggagttcggc gggagcgacg
    12421 gcgggctggt ggaacaggcc atcctccggg aagagatggc cgtggccaag gccccgccgc
    12481 tcggcacctc gttcatgggt ctggcctggg tgggccccgg catcatccag tacgggacgg
    12541 aggatcagaa acgccgcttc atccccgaca tcctggacag caaggtcatc tggtgcacgg
    12601 gctactccga gccgaaccac gggtccgacc ttgccgccat tcagtgcaag gccgtgcgtg
    12661 agggcgacca ctacgtcgtc aacggtcaga agatctggac cagcggcgcg ccgtggtccc
    12721 aatggatcat cctcctggta cggacggact tcgacgtgga cgtgaagcac aagggcatca
    12781 cgtgcctgct ggtaccgatg gattctccag gcgtcgaggt caagcccatc gagaacatgg
    12841 ccggtgaccg gcatttcgcc gaggtcttct ttaccgacgt gaaggttccg ctggagaacc
    12901 gtcttggcga cgagggagac ggctggaagg tcaccgtgtc cgcccttgcc aatgagcgct
    12961 ccagcatcgg cgaggtcacc cagatgttcg gcaagctcga cacgctcaaa gaactcgtca
    13021 ggaccactac caggcaggga cggcccgcaa gcgaggatcc caaggtcagg cggacgatcg
    13081 cgatgttcga aaccaagatc gccgcgatga agtacaacgg tttgcgttac ctgaccaagc
    13141 agatcaaggg cgagccgttg tccagcgaga catcggtgaa caagctgcat cgcgcaagcc
    13201 tcgagatcga gatggacgac ttcgcggtgg agctcaccgg tcaggccggg ctcctgctct
    13261 ccggcagcga ggaagccgtg tcaggaggaa gctgggcaaa gtcgagcctt tcctggccga
    13321 acgtggtgat cggcggcggc acgcccaata tccagcgcaa catcatcgcc gagcgcatcc
    13381 tcgggcagcc caaggactga cctagccggg ccaacgggcc cgccggtcag cgacgcttcc
    13441 agggcggcac ggcgtcccag tcgaagggct cgatgccgcc gtcggtgatc tgccagacgc
    13501 tgggccttgc ctccccgtcg acgaggtcga gaaaccccag cgtgccgtac tgggtatcgt
    13561 agttgtgggg ataggtgggc gagcccggat tcacgcagag cacgccctgc acctccgcga
    13621 tgatctcccg gtgggtgtcc cccgagacga tgatatcggg cgtccgctcg ggaaagtacc
    13681 gttcaaccca cctcgccaga gtgaactgtg gcgggcgttc gggcaccggc acgtaatgag
    13741 tcaggcccac ccagagaccc ccgagttcca gcagccaggc atacttcacg cgcggatcat
    13801 ccggctgcac gggtcgaccg ccgccaccat cctccccgtt gccccgggcg gcaaccaccg
    13861 gggctacctc ctccagggca tccaggacgt acagctcgtg aatgtccccc gcgtgcatga
    13921 tcatgtcgac gccgtcgaag gcggtgaaga cctgcggcca gagggttttg cgcgcctccg
    13981 gaatgtgtgt gtctgatatc aggccgatgc gcacgtcgac gctcacccgc ccgagacggg
    14041 ctgcaggtca tcgtccaccc gcttgatcga aggggacacc accagaagca acagcaccac
    14101 cacgatgaag atcgctccgc tgaccaccag cgccgtggcc acgctgaact gttcggcaat
    14161 gaggctgatg ggaatgacgc ccagcggcat gagcccgtgg gacatcatgt cgacgctgag
    14221 tatccgcccc cgcatgtggt cgtccacctg aagctggagc agcccgcggt tgagcgacat
    14281 gttccaggag ctcagaaagc cgatcaccgc gagcagcccg gcggagagca tcagggtctg
    14341 ggagaagccg aacgcgcagg tgaaggcccc ccagcctacg caggtagcaa tgagccaggc
    14401 cgcgcggtgc cggaagtcgt tcacgccggc cagcagcagg gatccgacga tggcgcccat
    14461 gcccatggtc gacatcagca gccccagatc gtccggcccc ccgcccagaa tgtcctcgtt
    14521 gaacgccggc agcagggtgt tgatgggcat gccgaacaga aacggcacga tggagagaac
    14581 gatcaggccg aataccggcg ggttggccca gacgtaacga aaggcgtccg ccatatcggt
    14641 gagcggggac ttgcgctgct cgcgggaact cacgatgccg ggcttgcgta ccagcaggac
    14701 ggtcgccatg gaaaggaagt agagcgcggc aatcacgacg tagacgatgc ctaccccgta
    14761 ggtggaggac gtgtcgcccg ccgcgaccca cgcgatgaga aagcctgcaa acgcggggcc
    14821 gagaatgcgt gagaggttcc agctggtggt gttcagcgcc atggccgtga agatcagcct
    14881 atccggcagg agctcgggcg cgaacgcctg gcgggcgggc atggaaagcg cgagaacggt
    14941 tccattgaag aaaccgaacc agacgaaatc ccagaaagac accagatcgg agaagatgat
    15001 cacacccatg acgatggtcg ccagggtgtt caacgcctgc gcgatggcga tgatggtgcg
    15061 cttcgggagc cggtcggcca ccacgccacc ccacagcgag aaggccacca tgggcaccat
    15121 gaacgacagg gtcacccagg ccaggtcaag agccgacgac gtcaacgtgt agaccagcca
    15181 gccccgggcg acgatctgca tgttcatggc gaaagacgaa gccaggctgc ccacccaaag
    15241 ccagcggaag tcgggcactt ccagagcttg gtagatgttt cgtgacgaca acggcggatc
    15301 cggtagggtc gggcaagccc gtcactttag cacagccgcg gggcgtcacc gtcccagccc
    15361 gaaccccgcg ggagggccgg ccgggcaata aaatcagtag tcgagccgga agctttcccc
    15421 gtcacgcacg atgtgcccgg cagtcggcgc ggcaaaatgg gtcccgatca cgagcacggg
    15481 acggtcggca tatcgctcga gaaacgcatc gcgcgtctcg gcgctggcct cctgattcca
    15541 gtccgcgctg ctcgaccatt ccggatgagc gatctggcag ggatgatgga tcatgtcccc
    15601 cgtgatgatg gcttcctctc ctgcggaagc aatgtgcacg ctcacgtgac cgggcgtgtg
    15661 cccgggcgtc ggctcgagcc agacctcgtc cgtgacccga tgggtctgcc ccaccacctc
    15721 ggcgagcccg gcatcgaaga tcggctgcac cgagtcctcc accacggggc cgaactcctg
    15781 cggctcgcgc tgccagtagg cccactcgtc ctcggcgaag agatagcgcg cccgggtgaa
    15841 agtgggtacc cacccatcgt ccgtcagccg cgtgttccag ccaacgtgat cgacgtgcat
    15901 gtgcgtgcac atcaccgtgt ctatggtctc cgggggatac ccggcctgag cgagccgatc
    15961 cagaaaatcg gtctgcatgc ggttccagcg cgggtaggtg cgctccttgt cgttgcctat
    16021 gcaggtatcg acgatcagcc gctccccttc ggactccacc acgagggcat ggacgctcaa
    16081 cactatccgg ccgtcgtcgt cgatgaacgg ccgaagccag ggtacctcga gaagcgcacc
    16141 cggatcggcc tggggcagca ggtacgggcc gagcgaggct gtggagagct ccacgatctg
    16201 cgtgaccgaa acctcaccaa tctgccattt cagcatggaa tccccctgac ctcaccccga
    16261 cccggcgagc cttgcagatc ggctgacggc ggtcaaatcc cgccgttccc cggccttggg
    16321 cgcggcatgc catgtcataa tgtcgctcgt cagctacata ccccggggga gagatcatga
    16381 ccagattcgg cgacatcgcg gtggagacca ccggacacgt ggcggtgctg gaaatacagc
    16441 gtccgcccaa caacttcttc gacgtggacc tgatcaacgc catgggggat gccttcgagg
    16501 ccatggacga ggatgccaac gtccgggcgc tggtcctggc cgccgacggc aagcacttct
    16561 gcgcgggcgc caacttcggc aaccgggacg agcagtcccg ccgccaggcg cgcacggcca
    16621 ccgacggcaa cccgctctac tcggctgccg ttcggctgtt cgcctgcagg aagccggtga
    16681 tcgccgcggt gcagggagcc gccgtcgggg gcggtttcgg ccttgccgtc atgccggact
    16741 ttcgggtcgt ctgtcccgag acccggttca ccgccaactt cgtgaagctc ggcttccacc
    16801 cgggcttcgg cctcacctac accctgcccc ggctcatcgg ccagcagcgc gccaatctga
    16861 tgttcatgac gggccggcgc atcgacggcg aaaccgcctg cgagtggggc ctggccgaca
    16921 ttctgacgga ccgggactcc ctccgtgccc gagccatgga gctcgctcag gaaattgcgg
    16981 agaacgcgcc gctcgcggtt acctcggttc gggcaaccct gcgtgagggc gtcgccgaag
    17041 ccgtgaagcg ccagaccgac cacgagttca tcgagcagca tcgcctgcag cagaccgagg
    17101 accacaagga aggcgtgcgc gccgtggccg aacgacggcc gggcaatttc aaggggcgct
    17161 agcggccctg ccggcgttcc gctcgtccag ggcgcgacgg atctctccgt gggcgtcctc
    17221 gtcgagatcg aagcgtttga gaagcagcga gccaatgctg tagcagacca gcggaaacag
    17281 gctgtacagc gtgatcatgg ccagctgaac cgtcatggtc tgttcctgat tgggcacgaa
    17341 tcccgatgcc tgcagcacga atcccgtgag cagaagcatg atgcccgtcg cgctcttgta
    17401 cacgaaattc caggccgcaa agtacgagcc ctcctttcgt tcccccgtca gatactcgtc
    17461 atagtcgatg acgtcaccct ggatggacgg cgacaccgtc ccaccgcagc cggcagcgaa
    17521 gccggcaaag acggcggcgg tggcgatgta ggccagcttg aggggcatgc tctccatgaa
    17581 cgggagcacg aacatgctgc caaacgcgac accggtgagc accagcgaga agatccagag
    17641 cctgatcttg ccgaaccgtc tcgaaagcgg aatccaaagg ggcaccgaga aggtggaagg
    17701 aatcatgtag cagaggatga tggcaggggc ccacatgggc gcgccgacca cgtactgggc
    17761 gacgtaaagt gtcagcgccc cgatggccgc ggagccgacg ttctcgatga aagacacggc
    17821 aatgatgatg cgggcatgcc ggttgcgcca aacgtcggca aatgcctgaa tcgggttctc
    17881 gttgaccctt ccctggtagt cggcgcgctc cctcagcttc cagacaccga aggccatgag
    17941 caggcacata acgccgccgg ccagaaccgc cagccagaag gccgtgtttc tgacctcctc
    18001 ggctccctgc tgctcagcgc tgatcagaaa gtagaagctg acgagagaga gaatcgagcc
    18061 gaaggtgaag cagccgtggc gaacgccgaa cagtcggctc cgctcgtggt agctctcgga
    18121 caactcggca cccagcgaaa ggtgcggcac caggaatccg gtcattgcgg agtagaaacc
    18181 gatgatggca acggccatcc aggccaccag ggcgccgtcg ctgaggccgt ccggcggcgc
    18241 gaagaccatg atgaaggcac cgccgatggg aatcatgctg cccagaatcc aggttcgcct
    18301 tcggcccatg cggctgcgcg tgcgatcgct gaggtagccg gccagcggat cggataccgc
    18361 gtcccagata cgcgatacgc tgaagatgac gcccatgacg gccggcgcga tgagcagcac
    18421 gtccgtggag aacttcatga cgtaaaggct catcatcagg tacatgtacc cagccccgat
    18481 accgggcgct ccgtaggcga ccacggcggt gttgggcacg cgagcgcggg ggtccgcgta
    18541 cggcgtcggc gagccgtctg ccacccgacc ccgcccgcgg ctcaggcgtt gcggatggtc
    18601 agaccgccat ccaccggcag ggctacgccc gtgatgaagc aggctgccgg caacgccagg
    18661 ctcaacgtgg cgtgggcgac ctcttcggga tccgcatagc gcttgagggc cacccggcgt
    18721 cgcgcgaact cggccttggc ttcgtcggga atggcagccg tcatgcccgt gaggatcggt
    18781 cccggacaga tacagttcac ggtgatgcct tcctcaccaa gctccaccgc cagggagcgc
    18841 gtcaacccga tgacgcccgt cttcgccgcc gtgtaagggc tgccgaactt cgtcgcgccg
    18901 agcccctcgg tggaagcgat gttgatgatc ctggcagcct cggccttgcg cagataaggg
    18961 agcgccgcgc ggatcgttcg agtatgcgcc gtgagcagca cggtcaagga tcgttcccag
    19021 gcgtgctcgt aatcatcgct gtcgatgggc gcgaacattg atatgcccgc gttgttcacc
    19081 agaatgtcga tgccgccgaa atgatcgggc acctctgcga acacctcgac aatggcgtcg
    19141 gggtcggaaa gatcgagcgc ccagcccttg atgtcacggc ccgcggcagc gatctcgtcg
    19201 acaacctcgt tgagcggctc agcgttgatg tccatggccg cgaccttggc gccctcgtcg
    19261 gcaaagaccc gcgccgtggc gcgccccata ccgctggccg caccggtgac gatgaccacc
    19321 ttgccttcca ccgatcggct gagcttgctg agcttcatat cgtgtacctc gtgacggccg
    19381 gagcggtctg cccgttcgat gcggtcccgt gccactctag ggagcaccat cgcacattgc
    19441 aatcggccgg ggaatcccgc taacgtaaac cacttcaccc accggcggga gttccctcga
    19501 agatggcaga agcctacgaa ttccacgacg acgacgtgat cgctccggag gtttacgggc
    19561 gccacggcca tccccatggc gcgtttgcct ggctccgcgc caacgacccg ctgcgctacg
    19621 tcgagcccac cgagtacctc cccttctggg ccgtgaccaa gcacgccgac atcgtcgaga
    19681 tcgagaagca gccggagatc ttcgcaagcg aaccaaggcc gatcctggtc aaggaggcac
    19741 agagcccgga ggtgcgccag gatgtcctga tgcagatctt cgagaggctc aaggactcgc
    19801 cgaagctcct ggaggtgctg gccaccatcg agcagggcgg gttgatccgc tcgctggtcc
    19861 agatggatcc cccagaccac ccgaagtacc ggacgctggt ccaaccctgg ttcaagccat
    19921 ccaacatcaa acgcctcgag gacagcttga cccgcatcat cgcaaacata ctcgacgaga
    19981 tgatgggcga cggctccgag cgcgagctgg agttcgtcca ggacgttgcc gtctggccgc
    20041 cgctcaagct gatcaccgag ctgctgggcg tgcccgagga ggacgagtgg cggatcctca
    20101 agctcaccaa cgagctgttt gccggcgacg acccggagat gaaacgggca ggcgacgatc
    20161 cgctctccat catcgacacc atcaaggacc tgtacgacta cttcacccgc ttgactgacg
    20221 aacggcgcgc caaccccacg gaggaccttt cctcgtacat cgccaacggc cgcatcgacg
    20281 gcgagtacct gccgtacaag gagctgatct cctactacat catcgtcgcg acggccggcc
    20341 acgagaccac gcgcacggcc atttccggtg gtctgctcgc cctgctcgaa aacccggagc
    20401 agatgagtct gctgcggaac aacatcgacg acgaggacct gatcaagctc gctgtggagg
    20461 agatgatccg ctggacgacg ccggtcagtc agttctcccg caccgccatg cgggactacg
    20521 agctgcgcgg caagcagatt cgccagggcg acgcgctggg cctgttctac gcctccgcca
    20581 accgggacga ggaaatcttc gacgacccct tcaatttcag ggtcgaccgc cagcccaacc
    20641 gtcacctcgc cttcgggacc gggcctcaca tctgtctcgg catcctgctg gcgcggctgg
    20701 agatgcggat attcttcaag cagttcctgc cccgcatcga gaagatggaa ctggtcgacc
    20761 ggggtccgga gtacctcaag gcaagcttcg tgcacgggct caagcacctg ccgattcggt
    20821 tccagctgag accggcgcgg tgagcacacg ggcaaccggg tcatgagcgc ggttctcgat
    20881 accctgggcg agcgctttgc cgcttacctg acaaagagct ggaagcagcc ggcaacgacg
    20941 ctgcacatcg agcagattcc cggcggcgct tccagggaaa cctaccgcgt cagggtttca
    21001 atcggtgggc aagagcaagg actcatactt cggcgcgatc ccgtttccag cctgatcgac
    21061 accgagcgtt ccctcgagta ccgaacctac gaggcagtat ttccgacgtc gatccccgtg
    21121 cctgagccgc tgctgctcga ggaggacgcc gtccacctgg aacggccgtt ttccgtgatg
    21181 cgcgagatcg gcggctgcga gtccagcgcg cagtccctcg cagcaccgcc ctaccaggcc
    21241 atgcgcgaga ccatcggccg gcgaaagtgg gaaattctcg gccagctcgc gtcgctcgac
    21301 ttaagcgagc tgggcgtcga cgccttcatg gcggtgccgg acgatcctgc acgacgggag
    21361 ctcgagcact ggtcgaaggt cattgccgac gacgcgctgc atccccagcc catcgcggaa
    21421 gctgcccgcc gctggctgga gcgaaatctg cccgagccgt cgggccgctt tgccctggtc
    21481 cacggcgact atcgctcggg caacttcctc tacgatgaag aaggcgtcat tcggggcgtg
    21541 ctggactggg agatggccca catcggcgat cccctggagg atctggcatg gtccctcgat
    21601 cccctgtggt catggcccga gcgcgagctt gccggctccc tgctgccgcg ggagcaggcc
    21661 atcggtattt gggaacaagc cagcggcatc accgtggaca gagacgcctt ccgctggtgg
    21721 caggtattcg cctcctacaa ggggctggcg atctggatct cctccaccga ggacttcgtc
    21781 aacggcgcgt ccaaggagcc catactggca gtggccggct ggctgatgag cgaccgtcag
    21841 aaccggattc tggtggaccg cttgaacccc cgctccgacc acgcctacgc ggagcccatc
    21901 gtatgattcc caacccctcg aaggctctcg tcgacctggc catgaagctt tccaccggcg
    21961 tgcttccgga aatcggctca ccttacgcgg cagcgaatgc gggcatgacg gccgtcctgc
    22021 tgatcatgct ctcacaggac agcgaacggg ccatcgcgaa ccgcatgaac gacatcgacg
    22081 agctcaaagc tcttttcgcg gacgccgcgg cgtttccgga tcgggtcggg ctcgcgtcct
    22141 tcgtcgatgc cgagcccgac tcgctcctgc tggcagacgt ggaccggctg cacgcagacg
    22201 gtatgaggct cctcatcgac ctgcacgcct gggcggaatc ccacgacgcc gggctcgatc
    22261 aggagatctg ggcattcctg ggacgccacg ccgatcggaa ccgcttcgac gtctgaacgg
    22321 gcgccgctcc catggaagcg accccaccgg caagggttga gatgggccct tacgctgact
    22381 acaattcgcg ctcttttttg ccgccatccg gcaagagggt cgttagctca gttggtagag
    22441 caccgggctt ttaacccgtt ggtcctgggt tcgagcccca gacgacccac cacctccact
    22501 catcgcgtcg gcgccagccc ggcaacctgg cgtggcagga tcccctttct tcgccccttc
    22561 cggtacaagc gatcggcccg ctttgcgatg tcggcgatca gaccggaatg ccgagcgaga
    22621 acgtcgcgcc acggcatctg aaagtaggct gccagaaatc gcaggcggtc cccccgggtc
    22681 agggcgatct cgtaagcgga gaaatagagg ctgcccagat ccttgaccag ccaccggtac
    22741 ggcacggcgt tccggatctc ggcgcgatgc aggtcaacca cgaagatgcg ggggtccggg
    22801 gcctgcggcc caaagagctc gggatgcagc atcagaaggt gacacaggta gtagtccctg
    22861 tgattgatac ccgccccgtg catggcgcgg gagatggcgg ccacctcctc gatcaaccgg
    22921 cgtttcagcc caggcgcggg cggctcctcc ggccagcgca gcacgtaggc ttcaaggctc
    22981 tttaccggtg caagctcgtc ggtgatgaga aacgaccggc gcctcgcggg gttacgccca
    23041 cgcagcccga agccggcgat ttccagggtg ctcaccccgg cagccgagag cgctcgagtg
    23101 gcacgatact cgttcccggc gtctatgacc gggcgacgca gcgtcagcag gttcttgaat
    23161 atttcccgcc agccggttcc aaaatgccgc ttggcgtaga acccggctcc gtcgagctcg
    23221 aagcggatgg tggagcggcc ggccatctcc cggacgctct caccgcgcag gtcgtccacg
    23281 acatcgaaga cgtccagccc atgccagcgc tggccgagat ccgatctgag ctcaatccgt
    23341 tcgcgcccgc tcatacggat cgggcggtgc ggtcaggccg cgtcctgatc acgagccacg
    23401 ctcgcccttc cggcgagtct cggcgcggcc gagtccggat tgcagaaagt aaggaccccg
    23461 tcctccagtg gagcgtgcct gatggccttt ttgtcctggg cataattctg ggtattgcgc
    23521 cagggatctt tctcgccctg cttcggaaac agatgcatgg agcgctgcat gtatcccggc
    23581 gtgaagtcgt cgatccactg cttgcccggc atgtcacgat cctgctcgcg caactgcgca
    23641 gcgacctggg aaacaccgag ctcgtccatg cggttgacaa gcctgcacac gtattctgag
    23701 gtaaggtcag cgcgcaacgt ccaggaggcg ttgatgtagc cgaacgtctg aaccaggttc
    23761 ggcacgccgg agaacatcat gcccttgtag gtgaacgtgt ccgggaagtt gactttggcc
    23821 tcgtcaaccc agaactcgac gcccgacatc acctcgagat tcaggcccgt cgccgtgacg
    23881 atgacgtcgg cctcgatggt ctgcccggac ttcagcctga ccccggagga ggtgaaggac
    23941 tcgatcaggt cggtcaccac ggtggccttg ccctggcgca gggcgcggaa aaagtcgccg
    24001 ttaggcacca ggcagagccg ctggtcccac gggttgtaac tcggcgtgaa gtgggtctgc
    24061 acatcgaagt ccggaccgag ctgtgcccgc actccacgga gcagacgatc ctttacccac
    24121 tccggattca ccctggtgcg gcgatagaag aactgctgca gggtcgtgtt cttccagcgg
    24181 gtgatcgcat acgccacctt ctccgggaag atccggcgga gccggttggc gatggcatcc
    24241 cgatcgggac gggaaaccat gtaggtcggc gatctctgca gcatggtgac gtgcgctgcc
    24301 cgctcggcga tcgccggcag cagcgtcacc gccgtggccc cgctgccgat tatcagcacg
    24361 cgcttgctcg aatagtcgag ctcatccggc cactgctggg ggtgaacgac gggaccctcg
    24421 aaatcggcca tgcccgggaa gtcgggcgtg tagccgcccg cgtagctgta gtagccgccg
    24481 cacatgaaga gaaagttgca ggagaactcc accacgccat cgggggtttc cacctcgagc
    24541 gtccaggtag ccgagtccga gctccagcgg gcggagcgca ggcggtggtt gaagcggatg
    24601 tgaggatcga cgtcgtgctc ccgcgccgct tcccgcacgt actccctgat ggacgggccg
    24661 tcggcgatgg ccttcggatt gcgccagggt ttgaagttgt acccgaacgt gtgcatgtcg
    24721 ctgtcggagc ggattcccgg atagcggaac aggtcccagg tacccccaat ggcatcgcgc
    24781 gactcgagaa tggcaaacgt cttccccgga catttctccc gaaagtggca ggcggctccg
    24841 atgccggata cacctgctcc cacgatgacc atgtcgaatt gctgcatctt gaagctcacc
    24901 tcaaccctgc cggcccgatg ccgacgccca cgtcaatggg cggatcacaa aggggcagga
    24961 ttgtgccgct tgcaaggctc atttgcaccc cgaccgttta cgcggcgggc accgtcgcct
    25021 tcctgaccac gtccaggtag ccgtgagaga agcagtcctt gtcgccgtaa aagccgcttt
    25081 ccttcagcca acgctgcatc ttcggcaggt gatagagcgg aacgctggca agcaggtggt
    25141 gctcgagatg gaagttcacg cgattcggag cgaccagcag gcgttcccac caggacgcca
    25201 ccgtgctgct cgtgttctcg cgggggtcgg cgctcgcata gtcaaagcac acaccgtgct
    25261 cacccatgta gcgtacgcgc gtcagcagcg ggtacacgaa gacgtaggcc gcccaccaga
    25321 gcaggtacgc ccacaaggcc cccacggcgg cgagagccgc gatcatcatc ccatgaagaa
    25381 ccaggaacgg cgcgttgcga cccggcttca tcacccgcag ctgccgccac gtgtcacgaa
    25441 tgcccgtctg cccagtgacg tcgcgaagca gcttgcgacg catgctgtcg cgcggggccg
    25501 ggtacatctg cgccaggtac aggtcgggat ccttgtccgt gcccgcgtaa cggtgatggc
    25561 cgaggtgata gtcccggtag cgatagaggt tggtattgac cagcccgccg cacagccagt
    25621 gccccacgat ctcgttgacc ctgcgccgcg cgaagaacac gctgtgggcg caatcgtgca
    25681 cgagcacttc catacccagc tgccgcccgc ccagaatcag cacgctcaac acgatggtca
    25741 gcgggtttgg ccaggctatg gaaagcgcga acgcggcgac gatcatcaga taattcacgc
    25801 ctagcacgcg ggcagcctgc cagtcagttc ggcgcaggca cgcctcgacc tgctcgggcg
    25861 tcagataatc ttcgatacgc ggcacgtcag ccaacgggca cctcctcgcc gtttcaccac
    25921 ggcatggcgg tatttattac acgttttgac cttaatagtc aaaatttgtt atttataagc
    25981 ggcatggacg ataccaacct caccgccagg cagctgatcc tcgccctgct cgattcagcg
    26041 cctgcgccgc agatccaggc cgcatcgctg atcgcggccg gcgcgctgtt cggtatggat
    26101 ccgggcgcca ttcgggtggc gacggcgcgt ctggtccgcg atcgggttct cgagagcacg
    26161 gcgcgggggc agtacaccgt gggcgggggc ggagccggcc tccaccggac cgtgctctcg
    26221 tgggcggatc tggaagaaac gctcaaaccc tgggccgggg aatggcttgc cgtctatctc
    26281 ggcagcctga agcgcaccaa caagaccctg gttcgcgccc gtgagcgagc attgcagctc
    26341 aaaggcttcg tcactgtcga cccagggctg gcggttcgcc ccgccaatct caaagcctcc
    26401 ctgcccgagt tgctcgagga cctgcgcctg ctcggtctcg acccgtccgc cggtctctat
    26461 cgcatcgcgg aaacggcaga cggctcccgc tttgctgggc tttgggacac cgacggtctc
    26521 gccaggcgct accagcgcca catcgacgag atcgcccgca gcacccgagg catagccgag
    26581 ctgtctcccc gcgcagccgc gaaggaaacg ctgctcgtcg ggcgcgccgc catcagggac
    26641 atcctccggg acccgctgct gcccgcggag ctcgtcgaca gccgtctgcg gcgggaactg
    26701 gtgcaccgga tgaaaaagta cgaccgtctc gggaagtcct gctggcgggc gttctacgcg
    26761 gacgtcggca aagccgatca gcggtaacgg gtcggatcct ccaccccggc ttcctcgaat
    26821 ccctgggcac gaagacggca gctgtcgcac ctgccacatg cggctgcgtc atcgtccggc
    26881 tgataacagg acaccgtgat gccgaagtcg acgccgagcg agatccccag ctccacgatc
    26941 ccggctttgc tgagctcgat gagcggcgcg tgaacatgat accaatcgcc ctccaccccg
    27001 gccttggtgg ccaggttggc aaggttctcg aaggcgaaga tgaaggccgg acggcaatcg
    27061 ggatagcctg agtaatctac ggcgttgacg ccgatgaaaa ggtcgcgcag cccgagcgtt
    27121 tcagcccaag ccagcgctag cgagaggaac acggtgttcc gggccggcac gtaggtgatg
    27181 ggaatgccct cgctgggctg ttcgggcacg tcgattgatc tgtcggtgag cgcagatccg
    27241 ccgaagacgg aaacgtccac cgggatcacg cggtgctcga tcacccccgc ccgtcgcgcg
    27301 acttccctgg ccgcagcgag ctccgcccca tgccgctggc cgtagtcgat cgagagcgcg
    27361 tagcagtcga agccctcgtc cttggccacg gcaagcgtag tggcggaatc cagcccgccc
    27421 gacaggagca cgatggcgcg cttcatcttc ccggttgatc tccccacagg tatttgtgca
    27481 gctggatctg catcctcacc ggaagccggt cccggacgat ccaatcagcc aggtcacggg
    27541 ctggcagctc cgcgctgctc ggcgagaaca gcacttcgca agcgccttcg aacagcttgt
    27601 gctcctcgca cttgagcctc gcccactcgt agtcctcacg atgacagatc acgaacttga
    27661 actgatcccc gtccgcgcaa tgcgcaagat tttcccatcg gttacgggac acctcgccgg
    27721 atcccggcgt tttgacgtcg atgatcttca cgacgcgctc gtccacgccg gccacgtcca
    27781 gcgcaccgct ggtctccaga gagaccgcaa atccctcgtc gcacagggag gtgagaagac
    27841 cgagacaatc gggctgagcc agcggttctc cgccggtcac cgtgacccgg cggacaccca
    27901 ggtcacgcac acgggccagg atgtcttcca gagtcatcac ctcgcccccg ctgaaggcgt
    27961 acgccgtgtc gcagtaatgg caccgcagcg gacacccggt gaggcggacg aagaccgtgg
    28021 gcaggccaac ggtgctggtc tccccctgga gggacagaaa aatttccgat atgcgaagct
    28081 ggcgcgttgc ctgttcggtc accgaatctc cgccgagtag gtccgggcga ggccggccgc
    28141 cgagctttgc gggtactcgg cggtcacgcg ctcgagatac tgcagcgcat tcgccgtgtc
    28201 accgagccgg tggtgaacta tgcccagctt gtagagggca tccggcgcct tctggtgatc
    28261 ggggtacagg ttgatcacct gggagaagga ttccctcgcc atctcgtcgt cggcctgggc
    28321 cagatgaagc tcgccaagcc agtaaaaagc gttgggcgtg aactgaccat tgggataatc
    28381 cgtgaccagc gcgttgaacg acgccaccgc ctcgtcgaac tgccggttct tcatctgatt
    28441 gaaggcggcc gtgtaagcat cgcgttccga gccgggcacg ccgccgggca ccgggccgcc
    28501 gggtggcggc gacgtctcgg ccagggtggg ttcgggcgcc tgcgcgcgca gcgcggccac
    28561 ccggcgatcc agatcgaggt actgctcctg ctgatccttg gcgagccggt tcagctggta
    28621 gctctgctct tccaccaggc cccggagatc ctggacctcc tgcctgagaa cctgcagctg
    28681 atagaagagc tccgccagcc ggcgctgctc gctggcgtcc ccggccggcg caggcacggc
    28741 ggaactctgg gtttcggtcg tcatctcggc cgcgaccggg ccgacgaccg aacctagaaa
    28801 cccggcgagg gccagcttca gcaccagccg cagacggtgt tttggcggtg acgtcaccac
    28861 ggtctagcgg tagatcatga tggcccggcg attccgcgcc caggcgccct cgtcgtggcc
    28921 cgggtcctcg gggcgctctt ccccgtagct cacggtttcg atctggcgtc gagagacacc
    28981 cgccgaggtc agaaaacttc gcatcgcgtc cgcccggcgt tcgccgagcg ccaggttgta
    29041 ctcccgggtg ccgcgctcgt cacaatgccc ctcgatgacg atcttgcggt ccgggttctc
    29101 ccttaagatg gaagcgtgca gctccaggga agccaggtcg tccgggttga tgaccgcacg
    29161 gtcgtactcg aagtagaacg tccgcgacag gagccgctgg gttcccggcg cgaaaggatt
    29221 gccgttcgga tcgaaggccg gcggcggcgt aggctggggt ggggccggcg gcggggtcgt
    29281 gtccaccgtc tcgacaggct cggccacggg ttccgctgga gcgggctggc ctccggttgc
    29341 gcagcccgcc agaaccgccc cgaaaaagaa aatcgcgagg agagtccgaa accaccgtgt
    29401 tgtcatctgt atctcctgtg ttgctggggt tccacggctg ctgagaactg ccaggctcgg
    29461 agcccgttca gtccaccctc tccagaacat cgagataagg ggaccaggcc ggctcccgaa
    29521 catcacccga ggaagagggc aatctgtact tcacgcggcc gtcgatcgaa acgacggcga
    29581 gtataccccg cccccgatcc tgcgtcgcat agattagcat cgtgccgttg ggcgcaagcg
    29641 acggagactc gtccagggac gtctgggtga gcaccctgac gtcgtcgcgc tcgaggtcct
    29701 gccaggctat gtgaaacacc ccctgtcgac ggtgtacgaa aataaggtgc ttgccgtccg
    29761 gcaggaggcg cgcccgggca ttgtagtcac cctgaaacgt gagccgctct atgaagttgg
    29821 tcgcgaggtc gagctggtag atctgtggcc tgccgccgcg gtccgaggtg aatatcacgc
    29881 ccttgccatc cggcgtccag ctgggctcgg tgtcgatggc gtggtgggtg gtgatgcggc
    29941 gcaggcgccg atcgtcgaga tccatgatat agatctcggg gttgccgtcc cgcgagagca
    30001 ccagagcgag ccgcttgcca tccggcgaga agacggggga accgttgatt cccgaaaact
    30061 gggtcagccg ctcccgcgcc cccgtggcga cgttctgcag cacgatggac ggccgtcctc
    30121 cctcgaagga tacgtaggcc acgcgggtcc catcgggcga ccagctggcc gaaaggagtg
    30181 gttgggtgga cgagaacagc gtccgcgacc gctcgccgtc cgagtcggcc acctccagcc
    30241 gaaacgcctc gtccggggac ccgacgttct gcgcgagaac gtacagaatg cgggtggaga
    30301 acgcgccggg aatgccggtg atcgcctcga agacctcgtc ggcgacgcga tgggccacgt
    30361 cccgccactg gctgcgcagc gccgtggtcg tgccgcccgt catccgacgc tcgttgaaca
    30421 cgtcgaacag ctcgtaggtg acctcgagat tgccggcggc gtccacgccc gtgcgcccta
    30481 tgacaagata ctcgatgccg agcacgcgcc agtcgcggaa gaagacttct tccctggtgg
    30541 tgggaaacga cagcatgttc tccatgccaa ggggcgaaaa ctggccgctg cgaacaaggt
    30601 cgaacccgat gatgtcaccc atctgctgac gatcggaaaa ctccggcccc tgccggaacg
    30661 gcaccacggc gatgcgcgtc tgcttgtcgt accccgcgtc gatgatgatg gtgtccaggc
    30721 tctccgcggc aagggtcgcg gccgacgtca gcatcaagac caggcagacg agcgcggaca
    30781 ggcagcgggc aaagttaatc aacgcagcag gtcctccgga cggaaaagca gtgtgaaacg
    30841 acggaagtag cgttcgaaga cgtccgaatc tttcggcacc tcgaaacgcc gtgcttttcg
    30901 cacggccgcc tcggccgagc ggtcgaatgc ctcgttgccg ctcccctcga tgagcgttac
    30961 cgaaacgacg tcgccagtcg gcacgagctc gacgagcaaa cgggcttgca tgttgtttcg
    31021 cgcgctgggt ggccgcgacc agttggcgac cacgcgttcg tagatcccgt aacggaaggt
    31081 ctgcgcgacg gcttcggcct cctccgcagc caggctctcg gcgagatcgg cggcctcggt
    31141 ctccagggcg tccagaaacg aggcttcggc gagctgctcg agacgctcct gccgctcggc
    31201 ctgtgcggct cgtctgcgct cggccccgac gtctggcgga ggcgcctggg gggggtccgc
    31261 aggtcgcgct tccgccttgg gcttcggctc ttcgggctgc cgggcaggca ggggtgaggc
    31321 cttggcggcg gctttcggtc tcgggacggg ttttgccttg ggctccatca tgaccagcgt
    31381 cgaccgcacg atctgcggct tgatggcgcg cacctcggcg gtctcggggt tccagccggc
    31441 ccggagagag agaaaggcca gagcgtgcag taccagcgcc agcagcagcg gcagcgcgta
    31501 gtcacggacg gccggcattg ctcgtcactc cccggcaagc tcgggcgggt cggtaatcag
    31561 accgacgctc tgcgcgccca cccgctgcag gacggtcatc acccggacca cggctccgta
    31621 gtccagcttg tgatcggcct tcacgtagac ggggacctcg ggacgcgcac gcaggatctt
    31681 gccggcctgg tcctccagcg agtagatcgt cacccgggtg ctctcgtcgt ccgcattctg
    31741 catgccgagg ttgacgaaca tggcgccgtc ctcacggatg gagaccacta ggggatcatc
    31801 ctgtcggtcc gtgatgggct cggaaggtgc tttcggcagg tcgacgatga cgccctgcgt
    31861 cagcagaggc gcggtggcca tgaagatcac gagcagcacg agcatcacgt cgatgtacgg
    31921 cacgacgttg atctccgaca tgggcttcct gcgcttggcc atgaacctgc cttcacctcc
    31981 ccccgcgggc atgcgccgcg cggtggagga tgccggtcaa ctcgtcgctg aacgcctcgt
    32041 aacggctcat catgacttcc acccgggcgg aaaaccggtt gtaaccgatc accgccggta
    32101 tcgccgcgaa gagccccatg gccgtcgcta ccagagcttc cgaaatgccc ggcgccaccg
    32161 aggcgagcgt cgcctgactc atgctggcca ggcttcggaa ggaattcatg atgccccacc
    32221 cggtcccgaa caggccgacg tacgggctcg tcgatcccac cgtagcgaga aaggccagat
    32281 gggtttcgag gttttcctct tcccggttca aggccacccg catggcccgc tgggctccct
    32341 gcatgatgga atccgcatcc gcaccggact gctccgacag ccgggtgtac tccttgaagc
    32401 cggccacgaa gatggattcg atacccgtga ggtcgtcttg ggcttccagc tcgttgtaca
    32461 acgcccgcag gtcgatcccc gaccagaagt gctcctcgaa gtcggcgatg gaagccagca
    32521 cccggcgcag gaagaaccat ctctgaaaaa tcatgaccca cgaggcgacg gacgcgagcg
    32581 cgagcagcag catgaccacc tgaaccagca ggctggcgtt ggcgatcagc tgaaatatgg
    32641 acaactgctc aggcacgatc gatcaactca cctcagatcg aggcgcggct ggccaccgga
    32701 gggctccgag gcttccggca cgctcaggcc gaagtgcagg tacgcctgct ccgtggccac
    32761 cctgccgcgg ggggttcgca tgaggtaacc gctctggatc agaaacggct cgaggacatc
    32821 ctcgatggta tcgcgctctt cgctgatggc ggccgccagg gagtcgaggc ccaccggccc
    32881 gccggagaat ttctcgatga tggccgagag caaccgtcgg tccatgctgt cgaatccctg
    32941 agcgtccact ttcaacatgt tcagagccgc gtccgcgatc ttccggttga tcaccccgtc
    33001 cgccttgacc tccgcccagt cgcgcacccg gcggagcagc cggttcgcga tgcggggcgt
    33061 tccacgagat cttcgcgcca cctcggtcgc gccctccgga tgaatctcca acccgagctt
    33121 cgccgcggat cgcgaaacga tctgcgtgag ttcttcgacc gtgtaaaact cgagtcgttg
    33181 cacgatgccg aagcgatctc gaagcggcga cgtcagcaga ccggcgcggg tggtcgcgcc
    33241 gaccagcgtg aatcgcggca gctcgagctt gagcgaccgg gccgccgggc cttcgccgat
    33301 gaggatgtcg agctgaaaat cctccagcgc gggataaagg atttcctcca ccaccgggct
    33361 gaggcggtga atctcgtcga cgaagagcac gtctccctcc tcgagattgg tcagcatggc
    33421 ggcaagctct gccggacgct cgagcaccgg cccggaggtg gacttgatgg ccacgcccat
    33481 ctcgttggcg atgatgtttg ccaacgtggt cttgccgagg ccgggcggcc cgaagatgag
    33541 cgtgtgatcc agcgcttcgt ctcgggcgcg ggctgcttgg atgaagatct ccatctgctc
    33601 cctgaccgcg cactgcccga cgtagtcgtc gagccgctcg ggccgcagcg cacgatcgaa
    33661 cccgccctca cggccatctt cccgggggct gaccaaccga tccggctcga cgctcacccg
    33721 ctcacctcct tgcgcatggc aatgtgcctc agggcggcac gcacgatctc ttccgtgctg
    33781 gcggaccctt cggccagcac gtcgctcacg acgcgctgcg cctcctgggg tttgtagccc
    33841 agcgccacca ggacctcctc ggcttcctca gccgacttgc ctcctcgggc cgggattcca
    33901 gcctcagcga ccacgacgat gtcggcgagc cggtccttga gttccaccag aagccgctcg
    33961 gccgttttcc tgcccacgcc gggaacccgg gtcagcaggg cgacgtcatt ggtctgcacg
    34021 gcgcgcgcca gatcggccag cggaatgctc gatatcaggg ccaacgccag cttggggccc
    34081 acgccggtaa tccggatcag ggccctgaag aggtcacgct cgtcccggct ggcaaagccg
    34141 tagagcagct gagcgtcctc gcgaaccaga aagtgcgtga agaggtcgac gggggcgtcc
    34201 gccccgggca accggcccag cgcggtgctg gtcaactcca cctcgtagca gataccaccg
    34261 gcgttgacga ggatcacgtt ttcggcgacc tcgacgagct gaccttggat tcgcccgatc
    34321 atgggcggga agtattgacg tgacaaatgg caatcgcaag cgcatccgcc gcgtcgggtg
    34381 ccggcgcccc gggaaggtcg aggatcgccc tgatcatgtg ctggacctgc tcctttcccg
    34441 cgcgccccga gccgaccacc gcctgcttga ccgtgcgggc cgcgtactcg tggacgggca
    34501 acccgcattg gacggcggcg gctatcgcca cgccgcgggc ctgtcccagc ttcagcgccg
    34561 agtcgggatt ccgggccatg aatacctgct cgatggccag cacctcgggg ccgtgctccg
    34621 agatcacgct ccggatgccg gtgtagatct ccgccagacg ctcgggaaac gctttcgcgc
    34681 ggggccggac gcagccgctg gcgagatagc ggatccggcc gccggacacc tcgaccaagc
    34741 cgtagccggt aatgcgggaa cccggatcga taccaaggat ccggagcgcc atcctcgcct
    34801 caggcgccca gcacgctgtc cggaaagtcg ccgttgctgt agacgttctg cacgtcgtcc
    34861 agctcttcca gcgcgtcgat gagctgcagc accttctcgg cgtcctccgg ctcgcacgcg
    34921 ctgtaggtcg aggccaccat cgtcacctcg gcgttgtccg gctcgagctg ctcggccttg
    34981 agcgctccga ccacctcgct cagcgactcc cagggggtgt agacggaaac cgagccgtct
    35041 ccctcggtct ctatgtcctc ggcaccggcc tcgagcacca cctccatgat ctgctcctcg
    35101 tcagcgcccg gggcgaagct gatgacgccc tgacgattga agagatacgc cacggacccg
    35161 tccgtgccca ggttccctcc gtgcttgctg aacgcatgtc ggacctccgc aaccgtgcgg
    35221 ttgcggttgt cggtcatggc ctcgacgaga atcgcgacgc ccgctgggcc gtacccttca
    35281 tagacgagct cttcgacgtc gctgccatcc tgccccccgg cgccccgggc gatggcgcgc
    35341 tctatggtgt ccttcggcat gtttgcgccc agggctttgt ccatggcggc gcgcaggcgc
    35401 gggttgtcgg ccacctcgcc gcccccgagc cgggcggcta ccgtgatctc acgaatgatc
    35461 ttcgtgtaaa gcttgccgcg cttgacatcc tgcgcggctt tcctgtgctt gatgttcgcc
    35521 cacttgctgt gaccggccat gcccgcctcc gattgatcct cagttcgtgc gcacgcggat
    35581 gtggagctct cgcaactgat gctcgtctac aggtccgggc gcatcggtca tgggacacga
    35641 cgccgtctgg gtcttgggaa acgcgatcac gtcgc
//
LOCUS       DBNHLM010000362        16482 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1372914, whole genome shotgun sequence.
ACCESSION   DBNHLM010000362 DBNHLM010000000
VERSION     DBNHLM010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 16482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16482
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1372914"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="AC16QF_16735"
     CDS             complement(<1..827)
                     /locus_tag="AC16QF_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450 family protein"
                     /protein_id="HIR6450256.1"
                     /translation="MTEINRMENPVCPASLAEATLADAEVLECPYPTYDLLREQAPVW
                     QDPRTGIFVVTRYEDLRMICTDTERFSNHRPSNDHDKLTGIARKAYELFVEKGWVPGE
                     SLAARDDPEHRQMRSIFNEAFRPKRINALDSEVQALAYRLIDDFVDDGHCNFVKQFAV
                     PLPLIIICRQMGAREEDVWQIKAWTDAWFRGTGFNLTEEEVIWATEMEIQAQHYFQPI
                     FERLRKEPDGTLLSDLVNTEVPGWGRTLNDNELHAEMMQDTFVGGSETTTNALSAGI"
     gene            867..1769
                     /locus_tag="AC16QF_16740"
     CDS             867..1769
                     /locus_tag="AC16QF_16740"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6450257.1"
                     /translation="MTMTSGKRPDAGRYQDVFFESDDGLDLYARDYPNPDAPLTVLCL
                     HGLTRNSADFADLAAALQPDFRVLAMDQRGRGRSAYDPDPSRYALPTYVKDAFALMDH
                     LALEHVAVIGTSMGGLMAMLMAARQPDRICGIVFNDVGPVVEAEGLRRIQGYVGKGGE
                     IASWADAVALTQANNGAAFPGLSEADWLAFARRLFVENDAGVPVLAYDAKISEPMQAD
                     QTTAVPADLWPVFDAMGEIPLLVVRGALSDILSRETVEEMARRHPGTTAVEVPDRGHA
                     PMLDEPEAMQAVSEFLAALGGKRG"
     gene            complement(1784..3067)
                     /locus_tag="AC16QF_16745"
     CDS             complement(1784..3067)
                     /locus_tag="AC16QF_16745"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6450258.1"
                     /translation="METEHQTGCPYRHADEIDFMDPVVQENWFDAYDIIREESPAYFM
                     PQIGMYVLTRYDDIEHVLRRPTQFTVGPDVQSTEPLIKHPEALALYEEKGWRRYTPLG
                     ENLPKHAHYRGLVDPALTAAAIRKRESFVRATVNELIDEWIDEGEIEFIDRFAEPLPM
                     IVIAELLGFPRMDLPQLKEWSYAWVLPFSRGLSLEQEMWAVNKHIELQHYIFETMQRK
                     RKDPKDDIITRLTRIEIEDVDIGAKRPLTDTEIIGITDHLLIGGNETTTFALSNGLWL
                     LFRNPDVYRALQADRDKIKNFVEEVLRIESPTQGLYRFVTEDCEIGGVPVPKGATLSI
                     RYGAGNHDPGRFPHPDTPELGRKNAGRHLAFGIGEHVCPGATLSRFEQRWAWEILLER
                     LRNIRPAPGKNDYKHRGGMWVRALEEIYMQFDKAG"
     gene            3178..3624
                     /locus_tag="AC16QF_16750"
     CDS             3178..3624
                     /locus_tag="AC16QF_16750"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6450259.1"
                     /translation="MIVGVHHVAIGVPDFETGLAFYRDVLGFEVLSASELSGNELADR
                     AIGLPDVRAQVAMLKAPNIYIELWSYSNPEPDDRRQRPCDFGYPHFALQVRDMQTEYA
                     RLSAAGMEFVGEPVELGGSAAIYGKDPFGNVIEIYEIRDPEIAQLS"
     gene            3713..3937
                     /locus_tag="AC16QF_16755"
     CDS             3713..3937
                     /locus_tag="AC16QF_16755"
                     /inference="COORDINATES: protein motif:HMM:NF016231.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HP1242/YdcH family protein"
                     /protein_id="HIR6450260.1"
                     /translation="MFEYEQDAVQALLKANDRFQDLYSQHAALKIQVHEAEIGVHPMD
                     DLTLNQLKRQKLLAKDKMAHMIQKYQRRHA"
     gene            complement(3977..4729)
                     /locus_tag="AC16QF_16760"
     CDS             complement(3977..4729)
                     /locus_tag="AC16QF_16760"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6450261.1"
                     /translation="MRLEGRTCVVTGGSSGIGAATVRRFVAEGAEVLIADIGVEQARG
                     LAAELGDAVSVVECDVRFEDSVRGVAAAAHARWDHVDVLVNNAGSELNRAYDEATVEE
                     WDRVQDTDLKGPWLMCKHLVPRMVERGSGSVINVSSLNGLVGFPLSTAYGGAKGGLVV
                     FTRDMAIELASSGVRFNCVCPGVIETPMMERWTDLMPDKGEAQEMLRGVMPIGRMGRA
                     DEVAGAILFFASDDSSLCQGAVLSVDGGFTAQ"
     gene            complement(4791..5660)
                     /locus_tag="AC16QF_16765"
     CDS             complement(4791..5660)
                     /locus_tag="AC16QF_16765"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6450262.1"
                     /translation="MKDFAGRTAVITGGASGIGLAMAQRFAEERMNVVLADIEENALR
                     AAVEHLEARQHRALGVVTDTMRRDAIEALLERAVAEFGNVHVLCNNAGVVVGGEHVPV
                     WEIPDADWEWVMGVNFYGVLYGLQTFVPHMLAHGEPGHIVNTASVAALMPGGDTYGVS
                     KHGVLVISEALHRDLRAAGSALGASVLCPGWVDTKIAEAERNRPTAMTSAKNPEGHGL
                     PLGDTLKQGMPPEVVAEKVLNSIREERFYILPHAGWDEVVLGRVDAIVARGAPYELDR
                     EALVAKRGLGEEV"
     gene            5761..7143
                     /locus_tag="AC16QF_16770"
     CDS             5761..7143
                     /locus_tag="AC16QF_16770"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIR6450263.1"
                     /translation="MSGKTRTEKDSMGALEVPADALWSAQTQRAVENFPISGIRMPGA
                     FIRSLGLIKAAAAHVNADLGVLPAELALPIVEAAERVAAGSVDDHFPVDVFQTGSGTS
                     SNMNANEVIARLASDASGLEVHPNDHVNAGQSSNDVIPTAIHVSAVLATREDLLPALA
                     HLAGVLRERAAGLQGVVKTGRTHLMDAMPIRLGQEVGAWATQVEQDIARIRDTLPRCE
                     RLAQGGTAVGTGVNAHPEFAVRFARRLSDVTGVPFLPSEDLPASLASQDAAVELSGQL
                     KVTAVSLMKIANDLRWMNSGPLAGIAEIMLPALQPGSSIMPGKVNPVIPEAIAMVCAQ
                     VIGNDATVTVAGQSGNFQLNVMLPVIAYNLIQSIELLGSGARLLADKALKEFEVAEDT
                     ISERLARNPILVTALNARVGYDAGAAIAKRAYAEGRSVLEVALEMTTLSREELERLLD
                     PAALAGPPRS"
     gene            complement(7168..7572)
                     /locus_tag="AC16QF_16775"
     CDS             complement(7168..7572)
                     /locus_tag="AC16QF_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0015671 - oxygen transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HIR6450264.1"
                     /translation="MSDLVLESLDAAAETGQDVTEAVNRAFHDREPQAAGLMDHMDEH
                     MLGRMMQEVLMVLMSENPEDQLEYLNFEVGSHLGYGVTAPMYGALFAAVRDCVRDLVG
                     EAWSPATETAWKQRIRSLQEIVETTAKAPADA"
     gene            complement(7565..8821)
                     /locus_tag="AC16QF_16780"
     CDS             complement(7565..8821)
                     /locus_tag="AC16QF_16780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HIR6450265.1"
                     /translation="MIARAPGRHALLFILITIFADTVAFGIIIPVLPKLIAELGDTSI
                     SEAASYGGLLMFVFAVMQFLFAPVIGNLSDRFGRRPVLLISLAALGIDYVIMGLAPSL
                     VWLFLGRMVAGVAAATFATANAYVADITPDEQRAARFGLIGAAWGVGFVLGPAIGGLL
                     GEMDMRLPFFAAAGLALANVVYGFVVLPETLARDRRRPFVLKRANPLGAIAGLARYPV
                     IPGLLVAIVLYFIGHDVNPSTWTYYVLHKFQWSEGQIGLALAAVGLSSAVVSGTLVGP
                     IVARLGEARTAYLGFCMAALSCLGYAFASQGWMIFPSIAVGAFMGLVMPSLRSIMSQM
                     VPEDGQGELQGAIASLMGLTAIFAPLIMTQVFRYFSAQEAGVYFPGASFLLAGLLMLL
                     GALVVMVVLRHPSAPPGQPSSETGDV"
     gene            complement(8876..9106)
                     /locus_tag="AC16QF_16785"
     CDS             complement(8876..9106)
                     /locus_tag="AC16QF_16785"
                     /inference="COORDINATES: protein motif:HMM:NF044586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450266.1"
                     /translation="MSKGTTSQSSNADLRRQLDADVEKFLKSGKKIQQIPTGVSSQDY
                     TKGRKHIVLGKSKPRTPETRTPETRTPEKGSE"
     gene            complement(9215..9622)
                     /locus_tag="AC16QF_16790"
     CDS             complement(9215..9622)
                     /locus_tag="AC16QF_16790"
                     /inference="COORDINATES: protein motif:HMM:NF013722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6450267.1"
                     /translation="MQVEAGDVIPEWVMERVAPERMRTMAAILRDPNPVHWDRKAVAA
                     LGFGDHTINQGPLGLSYMINMLHEWAGPHCLKRVFMRFPQVVLDEDHVIARGVVTAVR
                     ELDGAREADCEIWLEREGAGRLLEGSAVVRLPD"
     gene            complement(9612..10061)
                     /locus_tag="AC16QF_16795"
     CDS             complement(9612..10061)
                     /locus_tag="AC16QF_16795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450268.1"
                     /translation="MTMPDASEIEALVGHEFPGGKYTVAHWENFLLTECTGAEQLPEG
                     MVHPVVMFHMPILGAGTSIGEMFALGQAESDYSIGIESYDWEFFGTLEEDVEYSISAR
                     VTEADRRRSGDRVYDRIQFQFDVADPGGTVLARSTITWTYGRSGDAS"
     gene            10183..10734
                     /locus_tag="AC16QF_16800"
     CDS             10183..10734
                     /locus_tag="AC16QF_16800"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6450269.1"
                     /translation="MSKIDPYASWLPLEARAAAETNPRHKQLLLEVRDHMEHEIKGAL
                     EPLMATLTAEPVYHFWGNEPSVLNGRDAVRDFYKGMIAMGGQQFQVVVDRIVVDDGAV
                     VTEGQVKQVYKGAALKAMGMDAVGGERVADDDLFMTTAQLVTVWPADAVGKLIGEDIY
                     FGHDPFRNVERIGREDLPDYYQI"
     gene            10734..12443
                     /gene="ggt"
                     /locus_tag="AC16QF_16805"
     CDS             10734..12443
                     /gene="ggt"
                     /locus_tag="AC16QF_16805"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione gamma-glutamate
                     hydrolase [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR6450270.1"
                     /translation="MIPKTKTAYYGIGWLLMLLALTGQTALAASAAHRAQHGMVVTQS
                     AIASDVGARVLRAGGNAIDAAVATAFALAVTYPEAGNIGGGGFLVYQPQEGGADAYDF
                     RETAPTGSHPQMWLDEDGEYSPARQYVGPVAAGVPGTVAGLHKAWRDHGSLPWAELVA
                     PAIGLARDGFAIPEDLARSIAATLPQMEAYPASLAQFSRDGAPMAPGELLVQRDLART
                     LELIAADGPDAFYRGPIAEQIVALMASTGGLITAGDLASYRAVKRKPIRGTYRGYEIV
                     SMPPPSSGGVALVQTLNLLEGYDIAGEGFGSARTLHLITEALRRTFADRARYLADPDF
                     VKDMPVRRLTSKSYAAELRETISLNRAGTSSPETFEWPTASNDTAHFSVVDAQRNAVS
                     LTYTLNGRYGNQMVVPGAGFLLNNEMGDFNPVPGLTDERGHIGTTPNLAAPGKRMLSS
                     MSPTIVLKDGELFMVTGARGGRSIISTVLQTIMNVVDHGMNAQAAVDARRIHHQWLPD
                     RLRYEPQSFSRDTLNALRWLGHEIEQGGASWSNAEVIVVANDGWLEGGDDRRRSRGSV
                     AGY"
     gene            12485..13339
                     /locus_tag="AC16QF_16810"
     CDS             12485..13339
                     /locus_tag="AC16QF_16810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6450271.1"
                     /translation="MKIGLSNFPTEYSISAVALARAAEERGFESLWVVEHTHIPASRR
                     TPYPIGGDLPSIYWESYEPFTFLAQAAAVTTDLRVGTGICLVPEHHPIALAKRVASLD
                     ALSGGRFLFGVGAGWNAEELENHGVAFGDRWKVLREHVLAMKACWTEKDAEYHGRFVD
                     FDPVWVEPKPVQKPHPPIYIGASSTWAMERIVEYGDGWLPVPVPEFDERLALLNRLCE
                     ERGRDRSEIDVCLMTVVDSADTLAGIRDKGVNRVILSLPTLGEDEALRVLDGYANVVE
                     WAKEIGDG"
     gene            complement(13353..13928)
                     /locus_tag="AC16QF_16815"
     CDS             complement(13353..13928)
                     /locus_tag="AC16QF_16815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6450272.1"
                     /translation="MADQDRKKWDQRYAAGAYADRPHPSAILADWLPRLSAGDALDIA
                     CGAGRNALHMARAGFRVDAVDVSAEALARAASAAAEAGLEVNWMQRDLDTGLPVEGPY
                     DLIVMIRYVDESLLRALPALLRPGGVLIVEEHLTTEEPVIGPRDARFRVMPGALRAAV
                     PDLEVLEATDTVTDDPDGRRVALARLVARKP"
     gene            14149..14511
                     /locus_tag="AC16QF_16820"
     CDS             14149..14511
                     /locus_tag="AC16QF_16820"
                     /inference="COORDINATES: protein motif:HMM:NF039701.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HIR6450273.1"
                     /translation="MEDILVPISLFLVIGAVISLSFYFRYRTRQEIQSTVRAAIERGQ
                     ELTPEVLDGLSDALNSRHGDLRRGIISLAIGIALFLFAGLLGEPDAEGPLMAISAFPL
                     LLGIGYLAMWFFLRRKAG"
     gene            14530..15081
                     /locus_tag="AC16QF_16825"
     CDS             14530..15081
                     /locus_tag="AC16QF_16825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6450274.1"
                     /translation="MEVSDETLVQQVVRLKDLQAFERLVQRHEKRILHLQRRFIRDHA
                     LAEDLCQETFLRAWDRLETFSGRGSFAAWLTKLAYNIFLQHRRKHAARTSRETPYEEE
                     AEAGQTALEGPAPNADELPDLPRLLAILPEADRLVLLLSYGHGLSIAEIADVLETPDG
                     TIKSQIHRAKDKIRRHFSIGAAA"
     gene            15078..15425
                     /locus_tag="AC16QF_16830"
     CDS             15078..15425
                     /locus_tag="AC16QF_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450275.1"
                     /translation="MSTTNPGNDPHKDPLDALLSEAFQQDIEQVGDADMAGRVMRTLK
                     RRTRLRAAILGVSMAAGLSLATALALPGLTDLLRLLAEQAPVEPSSWGVLPIAALLAI
                     LTPWLFSLVDDRV"
     gene            15590..16144
                     /locus_tag="AC16QF_16835"
     CDS             15590..16144
                     /locus_tag="AC16QF_16835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6450276.1"
                     /translation="MTQPARETLAAMLQVHLGRLRRLALVLTENPDDAEDLVQDVVVK
                     LCGRPHEVAGVRDLSTWLNRVVYNQFIDDRRRRKRQPLQLVGSADELDAAMPGESAEP
                     MLEALCKDRDQRLGDAVNALNAEQRMVLLLHDSQGYRLTEIHQLTDIPLGTLKSRLHR
                     ARSRMRVLLDSERVPDDRSEPDQG"
     gene            16261..>16482
                     /locus_tag="AC16QF_16840"
     CDS             16261..>16482
                     /locus_tag="AC16QF_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015371271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR6450277.1"
                     /translation="MIYDNILQTIGNTPIVRLNRTGPDHVEMYVKVEAFNPLASVKDR
                     LAYAIIKDATDSGALQPGQTVVEATSGNTG"
BASE COUNT         2956 a         5587 c         5252 g         2687 t
ORIGIN      
        1 atgatgccgg cggaaagcgc gttggtggtg gtctccgagc cgcccacgaa cgtatcctgc
       61 atcatctcgg cgtgcagctc gttgtcgttg agcgtcctgc cccagccggg cacctcggtg
      121 ttcaccagat ccgacagcag ggtgccgtcc ggctctttgc gcagccgctc gaaaatgggc
      181 tggaagtagt gctgggcctg gatctccatc tccgtggccc agatcacctc ctcttcggtg
      241 aggttgaagc cggtgcccct gaaccaggcg tccgtccacg ccttgatctg ccagacgtcc
      301 tcctccctcg cgcccatctg ccggcagatg atgatcagcg gcaggggcac ggcgaactgc
      361 ttgacgaagt tgcagtggcc gtcgtcgacg aagtcgtcga tcagacggta ggcgagcgcc
      421 tgcacctccg aatccagcgc gttgatccgc ttggggcgga aggcttcgtt aaagatggag
      481 cgcatctgcc ggtgctccgg gtcgtcccga gccgccagcg attcgccggg cacccagccc
      541 ttctctacaa agagctcgta ggccttgcgc gcgatgccgg tgagcttgtc gtgatcgttg
      601 ctcggccggt ggttgctgaa gcgctcggtg tccgtgcaga tcatacgcag atcctcgtag
      661 cgggtgacca cgaagatgcc cgtgcgcggg tcctgccaga cgggggcctg ctcgcgcaga
      721 aggtcgtagg tgggataggg gcactcgagg acctcggcgt ccgccagggt ggcctcggcc
      781 aggctcgccg ggcagaccgg attctccatt cggttgattt ctgtcattcc attaccatgc
      841 gcatctttcg ccacgctaat gctgccatga ccatgacttc aggcaagcgc cccgacgcgg
      901 gccgctacca ggacgtgttt ttcgaaagcg acgacgggct ggatctttac gcccgcgact
      961 accccaaccc ggacgcgccg ctgaccgtgc tctgcctgca cgggctgacc cgcaactcgg
     1021 cagattttgc ggatctggcc gcggcgctcc agccggactt tcgcgtgctg gccatggacc
     1081 agcgaggccg gggccggtcc gcttacgatc ccgacccgag ccgttacgcg ctgccgacat
     1141 acgtgaagga cgcgtttgcc ctgatggacc acctggcgct cgagcacgtg gccgtcatcg
     1201 gcacctccat gggcgggctc atggccatgc tcatggccgc gcggcagccg gaccgtatct
     1261 gcggcatcgt tttcaacgac gttggcccgg tggtggaggc cgaaggtctg cgccgcatcc
     1321 agggctacgt gggtaagggc ggcgagatcg cctcgtgggc agacgcggtg gcgctgaccc
     1381 aggccaacaa cggcgcggct ttccctggct tgagcgaggc cgactggctc gccttcgccc
     1441 ggcgcctgtt cgtcgagaac gacgctggcg ttccggtgct ggcctacgat gcgaagatct
     1501 ccgagccaat gcaggcggac cagaccacgg ccgtacccgc cgatctctgg cccgtcttcg
     1561 acgccatggg cgagattccg ctgctggtgg tgcgcggcgc gctctcggac attctctccc
     1621 gggaaaccgt cgaggagatg gcgcggcgcc atcccggcac caccgctgtg gaggtgcccg
     1681 atcgcggcca cgcgccgatg ctggacgaac cggaagccat gcaggcggtg agcgagtttc
     1741 tcgccgcgct cggcggcaaa cgcggctgag gtcctgacag gcgctatccc gccttgtcga
     1801 actgcatgta gatctcctcg agggcgcgga cccacatgcc gccgcggtgc ttgtagtcgt
     1861 tcttgcccgg cgccggccgg atgttgcgca gccgctcgag caggatctcc caggcccagc
     1921 gctgctcgaa gcggctcagg gtggcacctg ggcagacgtg ctcgccgatc ccgaacgcca
     1981 ggtgccggcc cgcgttcttg cgacccagct ccggcgtgtc cgggtgcgga aagcgcccgg
     2041 ggtcgtgatt cccggcaccg tagcggatgc tgagggtagc gcccttggga acgggaaccc
     2101 cgccgatctc gcagtcctcg gtgacgaacc ggtagaggcc ctgagtgggg gattcgatgc
     2161 gcagcacctc ttcgacgaag ttcttgatct tgtcgcgatc ggcctgcaat gcccggtaga
     2221 cgtccgggtt gcgaaagagc agccacagcc cgttggaaag cgcgaacgtg gtggtctcgt
     2281 tgccgccgat cagcaggtga tcggtgatgc cgatgatctc cgtgtcggtg agcgggcgct
     2341 tggcgccgat gtccacgtcc tcgatctcga tccgggtgag ccgggtgatg atgtcgtcct
     2401 tcggatcctt ccgtttgcgc tgcatggtct cgaagatgta gtgctgcagc tcgatgtgct
     2461 tgttcacagc ccacatttcc tgctcgaggg acagcccgcg ggaaaacggc agcacccagg
     2521 cgtaggacca ctccttgagc tggggcagat ccatgcgggg aaaaccgagg agctcggcga
     2581 tgacgatcat cggcagcggc tcggcgaacc ggtcgatgaa ctcgatctcg ccctcgtcga
     2641 tccactcgtc gatgagctcg ttgacggtag cgcgcacgaa gctttcacgc ttcctgatgg
     2701 ccgccgccgt cagcgcaggg tccaccaacc cacggtagtg ggcgtgcttg ggcaggttct
     2761 caccaagcgg cgtgtagcga cgccagcctt tttcctcgta tagcgcgagc gcctcgggat
     2821 gcttgatgag cggttcggtg ctctgcacgt ccgggcccac cgtgaactgg gtcggccggc
     2881 gcagcacgtg ctcgatgtcg tcgtagcggg tcagcacgta catgccgatc tggggcatga
     2941 agtaggcggg cgactcctca cgaatgatgt cgtaggcgtc gaaccagttc tcctgcacca
     3001 ccggatccat gaaatcgatc tcgtccgcgt gccggtacgg acaccccgtc tgatgctcgg
     3061 tctccatgat gctcccctct cccttaaaac cctgccaact atacaattta tacaattgcg
     3121 ccccgaagag acggtaccat caaggccctc agcattcagc aacgcaaggg gatagcaatg
     3181 atcgtaggcg ttcaccacgt ggccatcggc gttccggatt tcgagacggg cctggccttt
     3241 tatcgggacg tgctcggttt cgaggtcttg agcgcaagcg agctctccgg aaatgagctc
     3301 gccgaccggg ccatcgggct gcccgacgtg cgggcccagg tcgccatgct gaaagcacca
     3361 aacatctaca tcgagctgtg gagctacagc aacccggagc cggacgacag acgccagcgc
     3421 ccgtgcgact tcggctaccc ccacttcgcg ctgcaggtca gggacatgca gaccgagtac
     3481 gcaaggctct cggcagccgg catggaattc gtcggcgagc cggtggagct cggcgggtcc
     3541 gccgccatct acggtaagga cccgttcgga aacgtcatcg agatctacga gatccgcgat
     3601 cccgaaatcg cccagctgag ctgacgacct ccgccgaaac agccccgcag tcttgtaagc
     3661 cacggaattt ttgactagtt tcagacgtgc acaacctggg aaggggtgaa gcatgttcga
     3721 gtacgaacag gatgcggtgc aggcgcttct gaaagcgaac gaccgctttc aggacctcta
     3781 cagccagcac gctgcgctga agatccaggt ccacgaggcg gagatcggcg tccatcctat
     3841 ggatgatctg accctaaacc aactcaagcg ccagaagctc ctcgccaagg acaagatggc
     3901 gcacatgatc cagaaatacc aacgccggca cgcctgaccc cgacccgcgg ggcctgccga
     3961 cgtccatctg cgcggcctac tgggcggtaa agcctccgtc cacggagagt acggcgccct
     4021 ggcagaggct ggagtcgtcg gaggcgaaga acagaattgc tccagcaacc tcatccgccc
     4081 ggcccatgcg gccgatgggc atgacgcccc gcaacatttc ctgagcctct cccttgtcgg
     4141 gcatcagatc tgtccagcgt tccatcatgg gggtctcgat gacgcccggg cagacacagt
     4201 tgaaacgcac cccggagctg gcgagctcga tggccatgtc gcgggtgaag accaccagac
     4261 cgcccttggc cccaccgtag gcggtcgaca gaggaaaccc caccaggccg ttgagcgagg
     4321 atacgttgat cacgctgccg ctgccgcgct ccaccatccg ggggaccagg tgcttgcaca
     4381 tgagccaggg acccttcaga tcggtgtcct gaacccggtc ccattcctca acggtggcct
     4441 cgtcgtaggc gcggttgagc tccgagcccg cgttgttgac gagcacgtcc acatggtccc
     4501 accgcgcatg ggccgcggcc gccacgccgc gcacgctgtc ctcgaagcgc acgtcgcact
     4561 ccaccacgct caccgcatcg ccgagctccg cggcaagccc gcgcgcctgc tcgacgccga
     4621 tgtcggcaat cagcacctcg gccccctcag ccacgaaccg gcgaacggtg gcggccccga
     4681 tgccgctcga cccgcccgtc accacgcacg ttctgccctc aagcctcatc ctgtctctcc
     4741 ttcaacctgg tcccccggcc cgcggctcgc gcctgccagg ccggcaatgc tcatacttcc
     4801 tcgccgagac cccgcttggc caccagggct tcgcgatcga gctcgtaagg cgcaccccgg
     4861 gcgacgatgg cgtcgaccct gcccagaacc acctcgtccc agccggcgtg ggggaggatg
     4921 tagaaccgct cctcccggat ggagttcagc accttttccg ccaccacctc cggcggcata
     4981 ccctgcttga gggtatctcc cagcggcagc ccatgccctt cggggttttt cgcgctagtc
     5041 atggccgtgg gccggttgcg ctcggcttcg gcgatcttcg tatccaccca ccccgggcag
     5101 aggacggacg cgccgagggc cgaaccggcc gcgcggagat cccggtgaag cgcctcgctg
     5161 atgaccagca ccccgtgctt gctgacgccg taggtgtcgc ccccgggcat gagggcggcg
     5221 accgacgccg tgttgacgat gtgcccgggc tcgccgtggg ccagcatgtg cggcacgaag
     5281 gtctggagcc cgtaaagaac cccgtagaag ttgacgccca tgacccattc ccagtcggcg
     5341 tccggaatct cccagacggg cacgtgctcg ccgccgacga cgacgcccgc gttgttgcag
     5401 agcacgtgaa cgttcccgaa ctcggcaacg gcgcgctcga gaagcgcttc gatagcgtcg
     5461 cgccgcatgg tgtccgtgac cacgccgagg gcccggtgct gacgggcttc gagatgctcg
     5521 accgcagcgc gcagcgcatt ctcttcgata tccgcgagca ccacgttcat gcgctcctcg
     5581 gcaaaccgct gcgccatggc aagcccgatg ccgctggcac ccccggtgat gaccgccgtg
     5641 cggcccgcaa agtctttcat gtccgctccc cgaacgagcg ttctaagata acaggattgc
     5701 gcggcgcctc tacaccgatc ggttaagatc ctgctgctgc cgcgcgatca ggagaaaccc
     5761 atgagcggaa agacgcgaac cgagaaagac agcatgggag cgctcgaggt gcccgccgat
     5821 gccttgtggt cggcacagac ccagcgcgcc gtggagaact tccccatcag cgggatcagg
     5881 atgcccggcg ccttcatccg ttcgctgggc ctgatcaagg ccgccgcggc acacgtcaac
     5941 gcggatctcg gcgtgcttcc ggcggagctt gcgctgccca tcgtcgaggc cgccgagcgg
     6001 gtcgccgccg gcagcgtgga cgatcacttc cccgtggacg tcttccagac cggatcgggc
     6061 accagcagca acatgaacgc caacgaggtc atcgcccgcc ttgcatccga tgcatcgggg
     6121 ctggaggtgc atcccaacga tcacgtgaat gccggccaga gcagcaacga cgtgatcccc
     6181 acggccatcc acgtcagcgc agtgctcgcc acgcgggaag acctgttgcc ggcattggcg
     6241 catctggccg gcgtcctgag agagcgggcg gccgggttgc agggcgtggt gaagacgggc
     6301 cgcacgcacc tcatggacgc catgcccatc cggttgggcc aggaggtggg ggcgtgggcc
     6361 actcaggtgg agcaggacat cgcccgcatt cgcgacaccc tgccacgctg tgagcgcctc
     6421 gcccagggcg gcaccgcggt gggaaccggc gtcaacgcgc atccggagtt cgccgtgcgg
     6481 ttcgcccgtc gcctcagcga cgtcaccggc gttcccttcc tccccagcga agatctgccc
     6541 gcaagcctcg ccagccagga cgctgccgtt gagctctccg ggcagctgaa ggtcaccgcg
     6601 gtgagcctca tgaagatcgc caacgacctg cgctggatga acagcgggcc gctggccggc
     6661 atcgcggaga tcatgctgcc ggcgcttcaa cccggcagca gcatcatgcc gggcaaggtc
     6721 aatccggtaa tccccgaagc catcgccatg gtctgcgccc aggtgatcgg caacgacgcc
     6781 acggtgacgg tggccggcca gtcggggaac ttccagctga acgtaatgct gcccgtcatc
     6841 gcgtacaacc tgattcagag catcgagctg ctcggcagtg gcgcccggct gctggccgac
     6901 aaggcgctca aggaattcga ggtggcggag gacacgatca gcgaacggct cgcgcgcaac
     6961 cccattctgg tgaccgcgct gaacgcgcgg gtgggctacg atgccggggc ggccatcgcc
     7021 aaacgggcgt acgccgaggg ccgctcggtg ctcgaggtcg ccctggagat gacgacgctc
     7081 agccgcgagg agctcgagcg cctgctggac cccgctgcgc tcgccggacc gccgcgaagc
     7141 tgaaccgccc gtccggcggc gcctcgctca cgcgtcggcg ggagctttcg cggtggtctc
     7201 gacgatctcc tgaaggcttc ggatgcgctg tttccaggcc gtttccgtgg ccgggctcca
     7261 ggcctcgccg accaggtctc gcacgcagtc gcgcaccgcg gcaaacaggg cgccgtacat
     7321 gggcgcggta acgccgtaac caaggtgcga gccgacctcg aagttcagat actccagctg
     7381 atcctcgggg ttctcggaca tcagcaccat gagcacctcc tgcatcatgc gaccaagcat
     7441 gtgctcgtcc atgtgatcca tgaggcccgc cgcttgaggt tcccggtcgt ggaaggcccg
     7501 gttgacggcc tcggtcacgt cctgaccggt ttcggccgcg gcgtccaagg attccagaac
     7561 cagatcagac atcgccagtc tccgacgaag gttggccggg aggcgccgaa ggatgcctga
     7621 gcacgaccat caccaccagc gcgccaagga gcatcagcag tcccgccaga agaaacgaag
     7681 cgccggggaa gtaaacgccg gcttcctgag ccgaaaaata tctgaagacc tgggtcatga
     7741 tcagcggcgc gaagatggcc gtcagcccca tgagactggc aatcgcgccc tgcagctccc
     7801 cctgcccgtc ctccggcacc atctgcgaca tgatgctgcg cagagacggc atgaccaggc
     7861 ccatgaacgc acccacggca atgctgggga agatcatcca tccctggctc gcaaacgcat
     7921 agcccaggca gctcagcgca gccatgcaga atcccaggta cgccgtgcgc gcctcgccca
     7981 gacgggcaac gatcgggccg accagggtgc cggaaaccac ggcgctggaa agcccgaccg
     8041 ccgccagcgc gaggccgatc tggccttcgg accactgaaa cttgtgcagg acgtagtacg
     8101 tccaggtgct ggggttgacg tcgtggccga tgaagtagag cacgatggca acgagcagcc
     8161 cgggaatcac cgggtagcgg gcaagcccgg cgatggcgcc cagcgggttc gcccgtttga
     8221 gcacgaaagg ccgccgccgg tcccgcgcaa gggtttccgg cagcaccacg aacccgtaaa
     8281 ccacgttggc cagggccagc cccgcggcgg caaagaaagg cagcctcatg tccatctcgc
     8341 cgagcagccc gccaatggcc gggccgagca cgaaccccac accccaggcc gctccgatca
     8401 ggccgaagcg ggcagcccgc tgctcgtcgg gcgtgatgtc ggccacgtag gcgttggcgg
     8461 tggcgaaagt cgccgccgcc acgcccgcga ccatgcggcc gagaaacagc cacacgaggc
     8521 tgggcgccag ccccatgatc acgtagtcga tgccgagcgc ggccagggag atcagcagaa
     8581 cgggccggcg accgaagcgg tccgaaaggt ttccgatcac cggcgcgaag agaaactgca
     8641 tgaccgcgaa gacgaacatc agaagaccgc cgtagctggc cgcttcgctg atggaggtat
     8701 ccccgagttc ggcgatcagc ttcggcaaca ccgggatgat gatgccgaac gcaaccgtgt
     8761 ccgcgaagat ggtgatgagg atgaacagca gcgcgtgccg tcccggcgcg cgcgcgatca
     8821 cgcgatcatg tcccgggctg gcacgccgac cccggcggga acatcagggt gacggctact
     8881 cggacccttt ctcgggagtc cgcgtctcgg gagtccgcgt ctcgggagtc cgcggtttcg
     8941 atttgccgag aacgatgtgc ttgcggccct tggtgtagtc ctgcgagctg accccggtcg
     9001 gaatctgctg aatcttcttg cccgacttta gaaatttctc gacgtccgca tcgagttgcc
     9061 ggcgaaggtc cgcattcgac gactgactgg tagtgccttt gctcatgatg aagagggtcc
     9121 gctaacgttc ggcgacgacg gtattcgccg tcactggaag atgcatcatg cccgcacgga
     9181 gcgagcaaag ccagcggacc tccccggaaa gcggctaatc cggcaaccgg acgacggccg
     9241 agccttccag gagccggccc gctccctcgc gctccagcca gatttcgcaa tccgcctcgc
     9301 gagctccatc cagctccctc acggcagtga cgacgccgcg ggcgatcacg tgatcctcgt
     9361 cgagtaccac ctggggaaaa cgcatgaaca cccgcttgag acagtgcgga ccggcccact
     9421 cgtgcagcat gttgatcatg tagctcaggc ccagtggacc ctggttgatg gtgtgatcac
     9481 cgaagcccag cgcggcaacc gctttgcggt cccagtgcac ggggttggga tcgcggagaa
     9541 tggcggccat ggtgcgcatg cgctccggcg ccacgcgttc catgacccac tccggaatga
     9601 cgtcgcctgc ctcaacttgc atcgccgctc ctcccatacg tccaggtgat ggtgctccgg
     9661 gcgaggacgg tgccccccgg atcggccacg tcgaactgaa actggatccg gtcgtagacg
     9721 cgatccccac tgcggcgacg atcagcctcg gtcacgcgtg cggagatcga gtactccacg
     9781 tcctcctcca gcgtgccgaa gaactcccag tcatagctct cgatgccgat ggagtaatcg
     9841 gattccgcct gacccagggc gaacatctcg ccgatgctcg tgccggctcc gagaatcggc
     9901 atgtggaaca tgaccaccgg gtgcaccatg ccctcgggca gctgctctgc gcccgtgcac
     9961 tcggtcagca ggaagttctc ccagtgagcg accgtgtatt tgccgcccgg aaactcgtgg
    10021 cccactagag cctcgatttc ggacgcgtcc ggcatcgtca tgccacattc cccttcttga
    10081 tccagcgacc aacattcaaa ccaatgaccg ggacctggtc aacggacggt tcagcctgac
    10141 gcgtcgggcc ggtaacatct gcagaccgca aaggagaccc agatgtcaaa gatcgacccg
    10201 tacgccagct ggcttcctct ggaagcgcgt gcagcggccg aaaccaatcc ccggcacaag
    10261 cagctgctgc tggaggtacg cgaccacatg gagcacgaga tcaaaggcgc gctggagcca
    10321 ctaatggcaa cgctgaccgc ggagcccgtc taccatttct ggggcaacga gccgtcggtg
    10381 ctcaacggcc gcgacgcggt cagggatttc tacaagggca tgatcgccat ggggggtcag
    10441 cagttccagg tagtcgtcga ccgcatcgtg gtggacgacg gcgccgtggt taccgaaggg
    10501 caggtaaagc aggtctacaa aggcgcagcc ctgaaggcca tgggaatgga cgccgtgggc
    10561 ggtgaacggg tggcggacga cgatctgttc atgaccaccg cccagctggt caccgtctgg
    10621 cccgccgacg ccgtcggcaa gctcatcggc gaggacatct acttcgggca cgatccgttc
    10681 cgcaacgtcg agcggatcgg ccgggaagat ctgcccgact actaccagat ctgatgatcc
    10741 ccaagaccaa gaccgcatac tacgggatcg gctggctgct aatgctgctg gcccttaccg
    10801 gccagacggc cctggcggcc agcgccgcgc accgcgccca gcacggcatg gtggtcaccc
    10861 agagcgccat cgcctcggac gtcggcgccc gggtgctgcg ggcgggcggc aacgccatcg
    10921 atgctgccgt ggcgaccgca tttgcgctgg cagtgaccta tccggaagcc ggcaacatcg
    10981 gcggcggcgg tttcctggtg taccaacccc aagagggcgg cgcggacgcc tacgactttc
    11041 gggagaccgc gcccaccgga tcacacccgc agatgtggct ggacgaagac ggcgagtact
    11101 cgccggcgcg gcagtacgtc ggccccgtgg ccgccggcgt gccgggaacg gtcgccggcc
    11161 tgcacaaggc atggcgcgat cacgggtccc tgccctgggc agagctggtg gcgcccgcca
    11221 tcgggctcgc ccgcgacggt ttcgccatcc ccgaggacct ggcccgcagc atagcggcca
    11281 ccctgccgca gatggaggcc tacccagcct ccctggctca gttcagccga gacggcgcgc
    11341 ccatggcgcc cggtgagctc ctggtgcagc gcgatctggc ccgaaccctg gaactcatcg
    11401 ccgccgatgg tcccgacgca ttctaccggg gcccgatcgc cgagcagatc gtcgcgttga
    11461 tggcctccac cgggggcctg atcaccgccg gggatctggc gtcctaccga gccgtcaagc
    11521 gcaagccgat ccgcggcacg taccggggct acgagatcgt ctccatgccg ccgcccagct
    11581 cgggcggcgt ggcgctggtg cagacgctca acctgctgga gggctacgac atcgccgggg
    11641 agggcttcgg ttccgcccgc actctgcacc tgatcaccga ggcgttgcgc cgcacgttcg
    11701 ccgatcgcgc gcggtatctg gccgacccgg acttcgtcaa ggacatgccc gtgcggcgcc
    11761 tgacatcgaa gtcctacgcc gccgagctgc gcgaaacgat ctcgctgaac cgcgcaggca
    11821 cgtcctcacc ggagaccttc gagtggccga cggccagcaa cgatacggcc catttctcag
    11881 tcgtggacgc ccagcgcaac gccgtgtcgc tgacctacac gctcaacggc cggtacggca
    11941 accagatggt ggtgccaggc gccggctttc tgctgaacaa cgagatgggc gatttcaatc
    12001 cggtgcccgg gctgaccgac gagcgcgggc acatcggcac cacacccaac ctcgcggcac
    12061 ccggcaagcg catgctctcg agcatgtcgc cgacgatcgt gttgaaggac ggcgagctct
    12121 tcatggtgac aggagcgcgg ggcggacgct ccatcatcag caccgtgctg cagaccatca
    12181 tgaacgtggt cgaccacggc atgaacgccc aggcggcggt ggacgcccgt cgaattcatc
    12241 atcagtggct gccggaccga ctgcgctacg agccgcagag cttttcgcgc gacacgctga
    12301 acgcgctgcg ctggctgggc cacgagatag agcagggcgg ggcatcctgg agcaacgccg
    12361 aggtcatagt ggtagcgaac gacggctggc tggagggcgg cgacgaccgc cgacgctccc
    12421 gcgggtccgt ggccgggtac tgagcgcacg cggcgaccgt ttcaactcaa tgaggagcaa
    12481 cgacatgaag atcgggctgt ccaactttcc caccgaatat tccatctccg ccgtggccct
    12541 tgcccgagcg gccgaggaac gcggcttcga gtccttgtgg gtggtggagc acacccatat
    12601 acccgcctcc cggcggacgc cctacccaat aggcggcgac ctgccgagca tctactggga
    12661 aagctacgag ccgttcacgt tcctcgccca ggcagccgcc gtgaccaccg acctgcgggt
    12721 cggcaccggt atctgcctgg tcccggagca tcaccccatc gccctggcaa aacgggtggc
    12781 gtccctggac gccctgtcgg gcggacgctt cctgttcggc gtgggcgcgg gatggaacgc
    12841 cgaggagctg gagaaccacg gcgtggcgtt cggcgaccgc tggaaggtgc tgcgagagca
    12901 cgtcctggcc atgaaggcat gctggaccga gaaagatgcc gagtaccacg gcaggttcgt
    12961 cgacttcgat cccgtctggg tggaacccaa gccagtgcag aagccccatc cgccgatata
    13021 catcggggcg tcctccacct gggccatgga gcggatcgtg gaatacggcg acggctggct
    13081 gccggtcccg gtacccgagt tcgacgagcg ccttgctctg ctgaaccggc tctgcgagga
    13141 acgcggccgt gaccgcagcg agatcgacgt ctgcctgatg accgtggtgg acagcgccga
    13201 caccctggcc ggcatccggg acaaaggcgt caaccgggta atcctgtctc tgcccacgct
    13261 gggcgaggac gaggcgctgc gcgtcctgga cggctatgcc aacgtcgttg agtgggcgaa
    13321 ggagatcggc gacggctgag ccggccggtt gctcagggct ttctggcaac cagccgggcg
    13381 agggccaccc tgcgcccatc ggggtcgtcg gtaaccgtgt ccgttgcctc gagcacctcg
    13441 agatcgggca ccgcggcgcg cagcgcgccc ggcatcaccc gaaagcgagc atcccgcgga
    13501 ccaatcaccg gctcttcggt ggtcagatgc tcctcgacga tcaggacccc tccagggcgc
    13561 agcaatgccg gcagcgctcg tagcagcgac tcgtcgacgt agcgaatcat gacgatgagg
    13621 tcgtagggcc cctccacggg caggccggtg tccaggtcgc gctgcatcca gttcacctcg
    13681 agcccggcct cggcggccgc cgaggctgcc ctggccagag cctctgcgga gacatcaacg
    13741 gcatcgaccc gaaaaccagc gcgagccatg tgcagggcgt tgcggcccgc cccgcaggcg
    13801 atgtccagag catcaccggc ggaaagccgg ggcagccaat ccgcgaggat ggcgctgggg
    13861 tgcggacgat cggcgtaagc gccggccgca tagcgctgat cccacttctt ccggtcctga
    13921 tccgccacgg gctctccagg tgatcgggcg ttcacgtgat tttcacagcg tccggggcac
    13981 atttcaccct ggacgccccg ggaaccggcc ctcccctctc cccacctcgg tcccggggca
    14041 tccttcatct ccccccaccg tctccgactt ttcgctcccg gcatgcaacc ggaagcggaa
    14101 aacgtgcatc agtccagtta gagccacaga aaattacgga gacagatcgt ggaagacatt
    14161 ctcgtgccca tctcgctgtt cctcgtcata ggagccgtca tctcactgtc cttttacttc
    14221 cgataccgga cccgccagga aatccagagc acggtccggg cggccatcga gcgcggccag
    14281 gagttgacgc ccgaggtgct cgacggcctc tccgatgccc tgaactcccg ccacggcgac
    14341 ctgcgtcgcg gaatcatttc gctggccatc ggcatcgcgc ttttcctttt tgcagggctt
    14401 ctcggcgagc cggacgcgga gggtcccctg atggccattt ctgccttccc gctgctgctg
    14461 gggatcggct atctcgcgat gtggttcttc ctgcgccgca aagccggatg acagtggtct
    14521 aatggcgtga tggaggtcag cgacgaaacc ctggtacagc aggtcgttcg gctgaaggat
    14581 ctgcaggcgt tcgagcgcct ggtccagcgg cacgagaagc gcatcctgca ccttcagcgc
    14641 cgcttcatca gggaccacgc cctggcggag gacctctgcc aggaaacgtt tctccgggcg
    14701 tgggaccggc tggaaacctt cagcgggcgg ggcagtttcg ctgcctggct caccaagctg
    14761 gcctacaaca tctttctgca gcaccgcaga aagcatgccg ccagaacgag ccgtgaaacc
    14821 ccatacgagg aagaggctga ggcagggcag acggcgctcg agggcccggc gccaaatgcc
    14881 gacgagttgc cggacctgcc tcgtctgctg gcgatacttc ccgaggccga ccggctcgtg
    14941 ctgctgctga gttacggcca cgggctgagc atcgcagaaa tcgccgacgt tctcgaaacg
    15001 ccggacggta cgataaaatc ccagatccac cgggcaaaag acaagattcg caggcacttc
    15061 agcatagggg ccgcagcatg agcactacca atcctggcaa cgatcctcac aaggatcctc
    15121 tcgacgccct gctcagcgag gcgttccaac aggacatcga gcaggtcggc gatgcagaca
    15181 tggctggccg ggttatgcga actctcaagc ggcgcaccag gctgcgcgcc gccatcctgg
    15241 gggtgagcat ggcagcgggt ctttcgttgg caacagccct ggccctgccg ggccttaccg
    15301 acctgctgcg cctgctcgca gagcaggccc ccgttgagcc ctcttcctgg ggagtcctgc
    15361 ccatcgctgc gcttctggcc atcctcacgc cctggctctt ctccctggtg gacgaccgcg
    15421 tctgacctga aggagcgcat cgcgacggcg cgctcgctca actttgcggg ctcgcgcaca
    15481 cttttccctt ccgccaggca cccattgtgg gcacacgttc gagcctttgc tggtccgagg
    15541 tgcaacactg atccgcgatc gcagtttgat caggaccacg aaacctccca tgactcaacc
    15601 cgctcgggaa acccttgcag ccatgctgca ggttcatctg ggcaggctcc gacgcctggc
    15661 cctggttctc acggaaaacc cggacgacgc cgaggacctg gtgcaggacg tggtagtcaa
    15721 gctctgcgga cggccgcacg aagtcgctgg cgtccgggat ctttccacat ggctcaaccg
    15781 cgttgtctat aaccagttca tcgacgaccg gcgccggcga aagcgccagc cgctgcagct
    15841 ggtcgggtcg gccgacgagc tcgatgccgc catgcccggc gagtccgccg agccgatgct
    15901 ggaggccctc tgcaaggacc gcgaccaacg cctgggggac gccgtgaacg ctctgaacgc
    15961 cgagcagagg atggtgctgc tcctgcacga ttcgcagggc taccgtctca ccgagattca
    16021 ccagcttacg gacattccgc tgggcactct gaaatccagg ctgcatcggg cccgctcccg
    16081 aatgcgcgtg ctgctggaca gcgagagggt cccggacgac cgatcagaac ctgaccaggg
    16141 ctgaccgaac cgccccggaa gaactcgaaa cgccccgacc ggctacgccc acctggatga
    16201 ggcaaacccc attggggtat cgtgggctgc agcgaagttc gcacacggag tggcatcccg
    16261 atgatttacg acaacatcct gcagaccatc ggcaacacgc cgatcgtcag gctcaatcgg
    16321 accggccccg accacgtgga gatgtacgtg aaggtcgagg cgttcaaccc gctggcatcc
    16381 gtgaaggacc gcctcgccta cgccatcatc aaggacgcca cggactccgg cgcgctgcag
    16441 ccgggacaga ccgtggtcga ggccacgtcg ggcaacacgg gc
//
LOCUS       DBNHLM010000363        17818 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1492716, whole genome shotgun sequence.
ACCESSION   DBNHLM010000363 DBNHLM010000000
VERSION     DBNHLM010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 17818)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17818)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17818
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1492716"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="AC16QF_16845"
     CDS             complement(<1..224)
                     /locus_tag="AC16QF_16845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6450278.1"
                     /translation="MSNIARLLTTAILIGVAALAGPTAAQESGLPRVVVQTSVGSFEV
                     ELYPDRAPASVANFLSLVDEGFYDGLIFHRV"
     gene            complement(246..1016)
                     /locus_tag="AC16QF_16850"
     CDS             complement(246..1016)
                     /locus_tag="AC16QF_16850"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003626.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HIR6450279.1"
                     /translation="MAVLSVNLNKVALLRNARGADQPSVTGAAKTVLAAGAHGITVHP
                     RPDQRHVRPDDVGDLADLLRAYPGIEYNIEGNPFAGPRDNGYPGFDALIESARPHQAT
                     LVPDTDNQLTSDHGWDLTGDNGRLSDHINRYQAAGARVSLFMDPVPEHIARARDVGAD
                     RIELYTGPFAQTVAEFGLDHEESLASLDRYQKAAACAAELGLGVNAGHDLDTHNLRLF
                     RQIEQVAEVSIGHALISDALEVGLTAAVRAYLDVLGHH"
     gene            complement(1020..1661)
                     /locus_tag="AC16QF_16855"
     CDS             complement(1020..1661)
                     /locus_tag="AC16QF_16855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HIR6450280.1"
                     /translation="MALKSKSCLAALLMLAGLTLSARAETVYISDTFAVPLRSGPSNS
                     HRILHRGLPSGTELTVLERDDEGQFARVRTTRGTEGWIPQQYLVAEPIARDRLDEASR
                     EIERLSSLVAEREAEIAQLRQAGARTWEEIEAGAQPGNRGPAGASAASPGERRLMELN
                     ERLRHEFERLAAERDELKSSARQRWLLIGAGIMLAGIAIGVILKARPRRSGWS"
     gene            complement(1645..2205)
                     /locus_tag="AC16QF_16860"
     CDS             complement(1645..2205)
                     /locus_tag="AC16QF_16860"
                     /inference="COORDINATES: protein motif:HMM:NF016189.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane-spanning protein YciB"
                     /protein_id="HIR6450281.1"
                     /translation="MNQLLDFIPIVLFVAVFFLADIYYATAALMIAVTLQVLAYRLLK
                     KPIGRELALTFWASVIFGGLTLVFRNELFIQWKPTIVNWLLCASLLGSHFVGQRNLME
                     RMLGHQLTLVDQVWVRLNFGWAFGFFLAGALNLVVAYNFSMEFWVSYKLIGGFGLTLV
                     YIVITLVYLSKKGFLTDKPEGLGIEK"
     gene            2305..2916
                     /locus_tag="AC16QF_16865"
     CDS             2305..2916
                     /locus_tag="AC16QF_16865"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR6450282.1"
                     /translation="MSQYFVVHPSHPQRRLLVTAAQIISGGGLVVYPTDTAYALGCHI
                     GDKQSLESIRRIRRLDKRHHFTLTCRDLSEISLYARVSDANYRILKHLTPGPFTFLLP
                     ATREVPRRLVHPKRRTIGLRIPNNVIALELLAVLGEPMMTTTMRLADQDEPFSDPEQI
                     RESLEHEVDLIIDGGPGGVVPTTVVDLTGAVPEVIRQGLGVFE"
     gene            2980..4194
                     /locus_tag="AC16QF_16870"
     CDS             2980..4194
                     /locus_tag="AC16QF_16870"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR6450283.1"
                     /translation="MSSNESSHRVLSGMRPTGRLHLGQYHGVIRNWLELQHGYECFFF
                     VADWHALTTGYEDTGNIEQNTFDMVIDWLAAGVNPGTATVFVQSWVPEHAELHLLLSM
                     ITPVSWLERVPSYKDQQARLNDRDLSTYGFLGYPVLQSADILMYRAGNVPVGADQVAH
                     VELTREIARRFNHVYGREPGFEDLAIAAIGKMGKKAARLYKSLRRGYVEQGDGEALER
                     ARALLAEQSNLSIGDRERLFGYLEGGGRIILPEPQALLNKAAKVPGLDGEKMSKSYQN
                     TIAMREAPDEVEKKVRTMITDPARVRRDDPGDPANCPVFALHEIYSDAETRAWVTEGC
                     TTAGIGCIDCKKPLIDAINAEQEAMRERAQQFEDDRDLVHSILVEGSEKARDVARETL
                     DDVRAAIGLAHR"
     gene            4191..5027
                     /locus_tag="AC16QF_16875"
     CDS             4191..5027
                     /locus_tag="AC16QF_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR6450284.1"
                     /translation="MSIDTEGAAAPHLLKLREQGNDTLALVRGEAVTELPKDLYIPPQ
                     ALEVFLETFEGPLDLLLYLIRRENLDILDIRVAEITAQYMSYIELMTAMQLELAGEYL
                     VMAAMLAEIKSRMLLPRPAYADEDEDDPRAELVRRLQEYEQIKNAAEGIDALPRLERE
                     IFIASAERPEVVKRHAEPDVDLKEVLVSLAEVLRRAEMFQHHEVYLEPLSVRERMTAI
                     LDRVNSASDFVSFGELFRAEEGRRGVVVTFLALMELVRESLLDFVQNEPYAPIYVRAA
                     GE"
     gene            5032..5742
                     /gene="scpB"
                     /locus_tag="AC16QF_16880"
     CDS             5032..5742
                     /gene="scpB"
                     /locus_tag="AC16QF_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR6450285.1"
                     /translation="MNEAVDDARLKAVIDAVLLAAGEPVSVNQILALFGPGELEGEDV
                     RARVRACLRELESEAAGRGYQLKRVASGYRYQVRQELSEWVSRLWDEKPPRYSRALME
                     TLALVAYRQPLTRGDIEQVRGVSVSPNIMRTLLERGWVRVVGQREAPGRPSMYGTTRA
                     FLDYFNLKSLDQLPPLEEIRALIEPIVVNDEAEEELRPEITVEMPADMTELAPEREDA
                     ESPEPMTAEVVPLSSLPQ"
     gene            5785..6606
                     /locus_tag="AC16QF_16885"
     CDS             5785..6606
                     /locus_tag="AC16QF_16885"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR6450286.1"
                     /translation="MSPEKSEKLQKHLADLAYGSRREMEAWIRAGRVEVNGEAAHLGQ
                     RISSEDRICVDGRPVTGKRTDWARVLVYNKPAGEICTRRDPEGRSTVFDGLPRLQRGR
                     WIAIGRLDLQTSGLLLFTNDGALADRMMRPATGLDREYAVRVNGKLSESQERRLTDGV
                     MVDGERLQFSDIKYYDGRGTNHWYHVVLMEGRNREIRRLFESVGLSVSRLKRVRFGPV
                     ALPSTLPRGRYAELSYEDLKTLYGLLKLPLRARPAKGGRGPRTSFLLPYPDLPIV"
     gene            complement(6609..6767)
                     /locus_tag="AC16QF_16890"
     CDS             complement(6609..6767)
                     /locus_tag="AC16QF_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450287.1"
                     /translation="MEDHNVGAKIFEDVFEFEDDFETEAEANVEAFYVALKMALARLE
                     AYATATPE"
     gene            6949..7362
                     /locus_tag="AC16QF_16895"
     CDS             6949..7362
                     /locus_tag="AC16QF_16895"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6450288.1"
                     /translation="MRHVGDILAEKSKDIVWIGPLESVYDALKLMNEKDVGAVLVQID
                     EYLVGILSERDYMRKVILDNHAAKQIKVSEVMTRDVVTVTREDTVVHCIALTKKHHVR
                     HLPVVEEGKAIGMISLRDLFLDVIHEKVGEVGDED"
     gene            complement(7402..8340)
                     /locus_tag="AC16QF_16900"
     CDS             complement(7402..8340)
                     /locus_tag="AC16QF_16900"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR6450289.1"
                     /translation="MAEIIARDAKFADPTTTADGKPRASVAPTGLTTLWVNTGTLCNL
                     TCENCYIESSPTNDRLAYISRAEVRAYLDEIAGSDLETEEIGFTGGEPFMNPDIMGML
                     EDSLGAGHRVLVLTNAMRPMMKHARGLLALGSRYGDALTLRVSVDHFEPDRHRQERGP
                     RSWRPMMEGLRWLSESGFAVHIAGRTRWGEDEHTLRAGFAALFAAEEIAVDAHDPASL
                     VLFPEMDTSADVPEITTECWDLLGRRPEDQMCASSRMVVKRRGAETPEVVACTLLPYD
                     ERFAMGPTLASSLRPVALNHPHCAKFCVLGGGSCSA"
     gene            8530..9294
                     /locus_tag="AC16QF_16905"
     CDS             8530..9294
                     /locus_tag="AC16QF_16905"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6450290.1"
                     /translation="MGRVEGKVAIVTGGASNPGLGRTTALTLAREGAIVVVTDVDLEG
                     AEATAAEIRDAGGRALALHQDVTDEGRWAEVIAATVDAHGRLDVLVNNAGIAVLMPLA
                     EMSLADWNRQIEVNLTSVFLGCKHAAPAMREAGGGSIVNLSSVAGLVGLPTCVAYGAA
                     KGGVRIMSKSVAVELAEHDIRCNSVHPGFIWTNMQAQSAGASAPGGGAMPRDRVPLGR
                     MGDPQDIANCVLYLASDESSYVTGAEFTVDAGMTAQ"
     gene            complement(9366..10166)
                     /locus_tag="AC16QF_16910"
     CDS             complement(9366..10166)
                     /locus_tag="AC16QF_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HIR6450291.1"
                     /translation="MPKSPEYPELEAARAGWRFQPEDDCLRGKVILVTGAGDGIGAAA
                     ARTYACYGADVVLLGRTRSKLETVFDWIEAETDTRPVIVPCDLRALDDASADALHDAI
                     ADGYGRLDGILHCASALGPKVPIALFPTADWQEVLQVNVTAPFLLTRTLLPLLNAAPA
                     ASVVLVSSTVGRKGRAYWGAYAVSKHALEGLMEVLADEHEHAGIIRVNSLNPGATRTA
                     MRAAAYPAENPASVPTPEARMDVFVYLMADASNGISGEALDAREWRGP"
     gene            complement(10150..10842)
                     /gene="ubiG"
                     /locus_tag="AC16QF_16915"
     CDS             complement(10150..10842)
                     /gene="ubiG"
                     /locus_tag="AC16QF_16915"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HIR6450292.1"
                     /translation="MNVDQAEIGKFDALAARWWDPEGEFKPLHDINPVRLNYLMEHCD
                     IRSRPVVDVGCGGGILAESLAREGATVTGIDMAESPLAVARLHAEDSGVQVEYLATTA
                     EDLAAERAGRFGTVACLEMLEHVPDYASTVKACADLAAPDGKLVFSTINRNPKAYLLA
                     ILGAEYVLGLLPRGTHDYDKFIRPAELAAAVRAAGLTVKEIIGMRYNPFTRQCSLTPD
                     VDVNYMLYAEKS"
     gene            complement(10839..12158)
                     /locus_tag="AC16QF_16920"
     CDS             complement(10839..12158)
                     /locus_tag="AC16QF_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HIR6450293.1"
                     /translation="MPSTPCDLIIDADWILPIVPAGSVLQEASIAIADGRILKIGDRE
                     LIHGEHQPREVHNLPGHALLPGLINAHGHAAMTLLRGFAEDAPLENWLSDHIWPTEAR
                     LVDADFVRDGARLAMAEMLKTGTTCFSDMYFFPEVTAAEARAAGMRAQIAFPVIEFPN
                     AWSASSDEGFHKGLALHDDYRRDPLIRVAFGPHATYSVSEGDLERILVYSEELDAHVQ
                     IHLHETAREVAEARDRVGMSWLRYLDERGLLGPNLQAVHATHLDDEEIDLLAENNVHV
                     VHCPLSNLKLASGICATTTLLARGVNVALGTDGAASNNGLDLLLEARLATLLAKLRDG
                     DAGALPAHRALEMATLGGARALGMEAQIGSLEPGKAADMVAIDLTGLKFQPLFDPVAQ
                     LVHTTSGDGVAHVWVAGRQLLRDGQLCTLDEQAILRAARGWRERISQ"
     gene            12294..14879
                     /gene="gyrA"
                     /locus_tag="AC16QF_16925"
     CDS             12294..14879
                     /gene="gyrA"
                     /locus_tag="AC16QF_16925"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020299636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR6450294.1"
                     /translation="MSDLGSDQGREILPVNIEDELRQSYLDYAMSVIVGRALPDVRDG
                     LKPVHRRVLFTMSELNNTFNRPYMKSARVVGEVMGKYHPHGDVAIYDTVVRLAQDFSM
                     RYMLVDGQGNFGSIDGDPPAAMRYTEVRMARIAGELLADLDKETVSFVPNYDDTLTMP
                     EVLPTRVPNLLVNGSSGIAVGMATNIPPHNLREVVAACLALLDDPSLTVDDLMVHIHG
                     PDFPTAAIINGRAGIVQAYRTGRGRIYVRARAEVISDGKSNKDTILITEIPYQLNKAR
                     LIEKIAELVKEKKIEGITELRDESDKDGLRVVIELRRGEPGEVVLNNLYAQTQLQSVF
                     GINCVALVDGQPRVLNLKQMLGAFVQHRREVVTRRSIYLLRRARQRGHILEGQAVALA
                     NIDAVVELIKASRTGADARTALMSRGWMSEAVQKLLERAGSDACRPEGLGEEFGFREG
                     LYYLSEEQAQAILELRLQRLTALEQDKLIEDYQVILDEIADLQDILGSEERLVSVIRA
                     ELEEIRDTYGDERRTEILSSHEDLSAEDLITEEDLVVTISHQGYAKTQSLDTYQAQRR
                     GGRGKAATAVKDEDFVEHLLIANSHATLLCFSNLGKVYWLKAYQIPRASRGAKGRPLV
                     NMLPLAADERITVVLPIEDFAEDRFVFMATANGTVKKTPLEQFARQRSTGLIALELEE
                     GNTLVGAAFTDGHCDVLLVSSSGKAVRFKESDVRAMGRTARGVRGIRLAGDHRVISLI
                     IPAPDGLILSASENGYGKRTVVDEFPVKGRGGQGVIAMQRSDRNGDVVGVVQVFGGDE
                     LMLISNMGTLVRTAADEVSVQGRNTQGVRLINLRGDEKLVGVERIVDSESAEADEESA
                     DADEE"
     gene            14876..15964
                     /gene="serC"
                     /locus_tag="AC16QF_16930"
     CDS             14876..15964
                     /gene="serC"
                     /locus_tag="AC16QF_16930"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HIR6450295.1"
                     /translation="MNRAINFSAGPAALPTQVLQQAQAELLDYAGTGMSVMEMSHRSA
                     EFIDIAERAERDFRELLQISDDYHVLFLQGGATLQFAMVPLNLLGDRGTADYVNTGSW
                     STKAIKEAARLCSVNVVASAEEHGFDRIPDPAAWACSADAAYLHICSNETIGGVQFHS
                     FPDRAGGPPLVADMSSDILSRPIDVSRFGLIYAGAQKNVGPAGLTLVVVRKDLVGAAR
                     PGTPSMLDYAVHAEAGSMSNTPPTYAWYLAGLVFAWIRSLGGVAALDELNRRKAGKLY
                     AAIDGSNLFRAPVVVEDRSIMNVPFTLPDGELDAAFLAGAKDRGLLNLKGHRSVGGMR
                     ASLYNAVPESAVDALISYMQEFETDHFR"
     gene            15961..17058
                     /gene="pheA"
                     /locus_tag="AC16QF_16935"
     CDS             15961..17058
                     /gene="pheA"
                     /locus_tag="AC16QF_16935"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR6450296.1"
                     /translation="MSTRDDRLKAVRDRIDAIDRELQKLLNERAACALEVGEIKQADP
                     DAEAPVFYRPEREAQILSRLKQENQGPLSDDDVARLFREIISCCLNLEQPLTIAYLGP
                     AGTYTEAATVKQFGHFAGTAALASIDEVFREVETGGAHYGVVPVENSTEGMVNHTLDC
                     FIDSPLKICAEVELPIHHAFMVAPDASGEGIEEILSHSQSLAQCRTWLDAHYPGVPRT
                     PVNSNAEAARLVAARTNAAAVAGDMAAERYGLRVIASHIEDQTDNKTRFLVIGQQRVG
                     PSGRDKTSILVSTRNEPGALYKILEPFHRHGISLSRIETRPARSGSWSYVFFIDFDGH
                     GSEPAIEAVMREVGDVAMEVRSLGSYPQAVV"
     gene            17059..>17818
                     /locus_tag="AC16QF_16940"
     CDS             17059..>17818
                     /locus_tag="AC16QF_16940"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR6450297.1"
                     /translation="MSPRVNPRIKALKPYQPGKPVEEVTRELGLVDVIKLASNENPRG
                     PGELVRQAIDAACGDLARYPDGNAFALKQALARHFGVRESQLTVGNGSNDVLDLAARM
                     SLSPGAEAVISEHAFVVYRLATLSAGGELVEVPARNYGADLDGILDAITDATAIVFLA
                     NPNNPTGTWVGEGALVRFLDEVPEHVWVVLDEAYHEYVEAAGYPNGMELQRRYPNLIV
                     TRTFSKIHGLAALRVGFSVSSEEAADLMNRARQPF"
BASE COUNT         3107 a         5679 c         5965 g         3067 t
ORIGIN      
        1 acccggtgga agatcagccc gtcgtagaaa ccctcgtcga cgagtgagag gaagttggct
       61 accgaggcgg gagcgcggtc cggatagagc tctacctcga agctgcccac gctggtctga
      121 acgacgactc tcggcagccc gctctcttgc gccgcggttg gaccggccaa ggcagcgacg
      181 ccgatcaata tggcggtagt caggagtcgt gcgatgttgc tcatgatgcc caatcgtccg
      241 gtaggtcagt ggtgtccgag gacgtccaga taagcgcgca cggctgccgt gagccccact
      301 tccagggcat cgcttatcag cgcgtgtcct atggagacct ccgcgacctg ctcgatctgt
      361 cggaacagcc gcaggttgtg ggtgtcgagg tcgtgcccgg cgttgacgcc gagcccgagc
      421 tcggcggcac aggccgccgc tttctgatac ctgtccaggc tggcgaggct ttcctcatga
      481 tccagcccga actctgccac ggtctgtgcg aacggaccgg tgtagagctc gatgcggtcc
      541 gccccgacgt cgcgggcgcg ggcgatatgc tctggcaccg ggtccatgaa gagacttacc
      601 cgggcgccgg cggcctggta gcgattgatg tggtcggata gtcggccgtt gtcgccggtg
      661 agatcccacc cgtggtcgga ggtcagctgg ttgtcggtgt cgggcaccag ggtcgcctgg
      721 tggggccggg cactttcgat gagcgcatca aaccccggat agccgttgtc tcggggaccg
      781 gcgaaggggt tgccctcgat gttgtactcg ataccggggt atgcccgcag caggtcagcc
      841 aggtcgccca cgtcgtcggg gcgcacgtga cgctggtccg ggcgtgggtg cacggtgatg
      901 ccatgggcgc ccgcggcaag caccgtcttc gccgctccgg tgacgctggg ctggtcagca
      961 ccgcgggcgt tgcgcagcag ggccaccttg ttcaggttta cgctcagaac cgccaaggat
     1021 cagctccagc cgctgcgacg gggacgggct ttgaggatca ccccgatggc gatgcctgcc
     1081 agcatgatgc cggctccgat cagcagccag cgctggcgcg cgctgctctt cagctcgtca
     1141 cgctcggcgg ccagccgttc gaactcgtgg cgcagacgct cgttcaactc catcaggcgc
     1201 cgctcgccgg ggctggcagc gcttgcgcct gcagggccgc ggttgccggg ctgggcgccg
     1261 gcctcgatct cctcccaggt tcgcgcccct gcctggcgca gctgggcaat ctcggcttcc
     1321 cgctccgcga cgagactgct cagccgctcg atctctcggc tcgcctcgtc gagccgatcc
     1381 cgcgcgatgg gctcggcaac gaggtattgc tgcgggatcc agccttccgt tcctcgggtg
     1441 gtccgcaccc gggcgaactg gccctcgtcg tcccgttcca gcacggtcag ctccgttccg
     1501 ctggggagcc cccggtggag tatccgatgc gagttcgacg gtccgcttcg aagcgggacc
     1561 gcgaaggtgt cggagatgta gaccgtctcg gcccgggccg acagggtgag gccggcgagc
     1621 atgagcaggg ctgccaggca ggatttactt ttcaatgcca aggccctctg gcttatccgt
     1681 gagaaaacct ttcttgctca ggtataccag cgtgatgacg atgtagacga gcgtgaggcc
     1741 gaaacccccg atcagcttgt agctcaccca gaactccatg gagaagttgt acgccaccac
     1801 gaggttgagc gcgccggcga gaaagaagcc gaacgcccag ccgaagttga gtctcaccca
     1861 cacctgatcc accaacgtca gctggtgacc gagcatgcgc tccatgaggt tgcgctgccc
     1921 cacgaagtgg ctccccagca gcgatgcgca cagaagccag ttgacgatgg tcggcttcca
     1981 ctgaatgaac agctcgttgc ggaaaaccag ggtgagcccg ccgaatatga cgctcgccca
     2041 gaaggtcagc gccagctcgc ggccaatggg tttcttcaaa aggcgatagg cgagaacctg
     2101 cagggtcacg gcgatcatga gcgctgccgt ggcgtagtag atgtcggcaa ggaagaaaac
     2161 ggccacgaac agcacgatgg ggatgaagtc gagcagctga ttcatcgttg attggccgaa
     2221 tagccccgct gcgcgcgggg atcctggata aagctaagcg tgtgataata cgagaaaccg
     2281 agtgggcatg gtaagcaggg gtctgtgagt cagtatttcg tcgtgcatcc ttcgcacccg
     2341 cagcggcggc ttctcgtcac cgcggctcag atcatctcgg ggggcgggct ggtcgtctat
     2401 ccgaccgata cggcctacgc gctcggatgc cacatcggcg acaagcagtc gctggagagc
     2461 attcgtcgaa tccgccgcct ggacaagcgt catcacttca ccctgacctg ccgggatctc
     2521 tcggagatct cgctgtacgc ccgcgtttcg gatgcgaact accgcattct caagcacctc
     2581 accccgggac cgttcacgtt cctcctgccg gcgacccggg aggtcccacg tcggctggtt
     2641 cacccgaagc ggcgcacgat cggcctgaga atcccgaaca acgtcatcgc gctcgaactg
     2701 ctcgccgtgc tcggcgagcc catgatgacg accaccatgc ggcttgccga tcaggacgag
     2761 cccttttcgg atccggagca gatccgggag agcctggagc acgaggtgga cctcatcatc
     2821 gacggcggcc cgggcggcgt ggtgcccacc acagtcgtcg acctcaccgg agcggtgccg
     2881 gaggtgatac ggcagggctt gggcgtgttc gagtgaacgg gtggttttgc cgggccccag
     2941 ccctataatg ccgcctcagt ttgtagttga gaggctgcgt tgagcagtaa cgagtcgagt
     3001 caccgggtac tctcggggat gcgtccgacc ggccggctgc acctcggcca gtatcacggc
     3061 gtcatcagga actggctcga gctgcagcac ggctacgaat gtttcttctt cgtggcggac
     3121 tggcacgcgt tgactaccgg ctacgaagac accggtaaca tcgagcagaa caccttcgac
     3181 atggtgatcg actggctcgc tgcgggtgtc aacccgggta cggccaccgt cttcgtgcag
     3241 tcctgggtgc ccgagcacgc cgagctgcat ctgctgctgt cgatgatcac cccggtgagc
     3301 tggctcgagc gggtgccgag ctacaaggac cagcaggccc gcctgaacga ccgcgacctt
     3361 tcgacctacg gctttctcgg gtaccccgtt ctccagtctg cggacatact gatgtacaga
     3421 gcgggcaacg tgcccgtggg tgccgatcag gtggcacacg tcgagctgac ccgggagatc
     3481 gcccgccgct tcaatcacgt ctacgggcgg gagccggggt tcgaagacct cgcgatagcg
     3541 gccattggca agatgggcaa gaaggccgcg cggctctaca aaagcctgcg acgaggatac
     3601 gtcgagcagg gggacggcga ggccctcgag cgagcgcgag ccctgctggc ggagcagtca
     3661 aacctgtcca ttggcgatcg cgaacggctc ttcggctacc tcgaaggtgg tgggcgtatc
     3721 atccttcccg agcctcaggc cctgttgaac aaggcggcga aggtgcccgg gctggacggg
     3781 gagaagatgt ccaagtccta ccagaacacg atcgccatga gggaagctcc cgacgaggtg
     3841 gaaaagaagg tccgcaccat gattaccgat ccggcgcgcg ttcgccgtga cgatccgggc
     3901 gatccggcga actgtcccgt attcgcgctg cacgagatat actcggacgc cgagacccgc
     3961 gcctgggtga ccgaaggctg caccaccgcg gggattggct gcatcgactg caagaagccg
     4021 ctgatcgatg ccatcaacgc ggagcaggaa gccatgcgcg aacgggcgca gcaattcgag
     4081 gacgaccgcg atctggtgca ctccatcctc gtggaaggct ccgaaaaggc ccgcgacgtc
     4141 gcgcgggaaa ccctcgacga cgtgcgcgct gccataggac tggcccaccg atgagcatcg
     4201 acacggaagg cgctgccgct ccccatctgc tgaagcttcg tgagcagggc aacgacaccc
     4261 tggcgctcgt tcggggcgaa gcggtaaccg agctgcccaa ggatctctac attcctccgc
     4321 aggcgctcga ggttttcctc gagacgttcg agggcccgct cgatctgctt ctgtacctga
     4381 tccgcaggga gaatctcgac attctggaca tccgggtcgc cgagatcacc gcgcagtaca
     4441 tgagctacat cgagctgatg acggccatgc agctggagct ggccggagag tacctggtga
     4501 tggccgccat gctggcggag atcaaatcga ggatgctgct tccacgtccg gcttatgccg
     4561 acgaagatga agacgacccc cgtgcggagc tggtccgccg gctgcaggag tacgagcaga
     4621 tcaagaacgc ggcagagggc atagatgccc tgccgcgact ggaaagagag atattcatcg
     4681 ccagtgccga gcgcccggaa gtcgtgaagc gccacgcgga gccggacgtc gacctcaagg
     4741 aggtgctcgt ttcgctggcg gaggtgctgc ggcgggccga gatgtttcag catcacgagg
     4801 tgtacctgga gccgctatcg gtacgcgagc gcatgacggc catcctcgac cgggttaatt
     4861 cggcaagcga ctttgtctcg ttcggcgagc tgttcagagc cgaagagggg cgcaggggtg
     4921 tggtcgtcac cttcctggcg ctgatggagc tggtgcgcga atccctgctg gatttcgtcc
     4981 agaacgaacc gtacgcgccc atttacgtgc gcgcggctgg agaatgagcc catgaacgaa
     5041 gctgtggacg atgcgagact caaggccgtc atcgatgcgg tcctgctcgc ggcgggagag
     5101 ccggtatccg taaaccagat cctcgcgctg tttggccccg gcgagctcga aggtgaagac
     5161 gtcagagccc gggtgcgcgc ctgtctcagg gagctggagt ccgaagcggc cggcaggggc
     5221 taccagctca agcgcgtggc cagcggctac cgctatcagg tccgtcagga gctcagcgag
     5281 tgggtgagtc gtctgtggga cgagaagccg ccgcgctatt cccgggcgct gatggagact
     5341 ctcgctctgg tggcgtacag gcagcccctg acccggggcg acatcgagca ggtccgcggc
     5401 gtcagcgtga gccccaacat catgcgaacg ctgctcgagc gtgggtgggt tcgcgttgtc
     5461 ggtcagcggg aagcgcccgg gaggccctcc atgtacggga ccacgagagc gtttctcgac
     5521 tatttcaacc tcaaaagcct cgaccagctg ccgccattgg aggagatcag ggccctaatc
     5581 gagcccatcg tcgtcaacga cgaagccgaa gaggagctgc gcccagagat caccgtagag
     5641 atgccggccg acatgacgga gctcgcacca gagcgggaag acgccgagtc gccagagccc
     5701 atgaccgcag aggtggtgcc gctttcgagc ttgccgcaat aggggatcct tccagccctt
     5761 aggggcgttg gcccccggaa aacgatgtcc ccggaaaaat cagagaaact gcagaaacac
     5821 cttgccgacc tggcttacgg ttcgcggcgt gagatggaag cgtggatccg ggccgggcgg
     5881 gtcgaggtga acggcgaggc ggcccatctc ggccagcgga tctcgtctga agaccgcatc
     5941 tgcgtcgatg ggcggccggt cacgggcaag cgcaccgact gggcgcgcgt gctcgtctac
     6001 aacaagcccg ctggtgagat ctgcacccgc cgtgaccccg agggccgcag caccgtcttc
     6061 gacgggctgc cccggctgca gcgcggccgc tggatcgcca tcggccggct cgatctgcaa
     6121 acgagcgggc tcctcctctt caccaacgac ggggcccttg ccgatcgcat gatgcgcccg
     6181 gcgacgggtc tcgaccggga atacgcggtc cgcgtcaacg gcaagctcag cgaaagccag
     6241 gagcgtcggc tcaccgatgg ggtgatggtc gacggcgagc ggctgcaatt ctcggacatc
     6301 aagtactacg acgggcgtgg caccaatcac tggtatcacg tggttctgat ggagggccgg
     6361 aatcgtgaga tcaggcgact gttcgagagt gtcggcctgt cggtcagccg gctcaagcgg
     6421 gtccggttcg gtcccgtggc gcttccttct accctccctc gcggacgcta tgccgagttg
     6481 agttacgagg atctgaaaac cctgtacggc ctgctcaagc tgccgctgcg ggctcgcccc
     6541 gccaagggcg ggcgtggtcc gcgcacctct tttctgctgc cgtaccccga cctgccgatc
     6601 gtctgaggct attccggcgt ggcggtcgcg tacgcttcga gccgggcgag tgccatcttg
     6661 agggcgacgt agaacgcttc cacgtttgcc tctgcttcgg tttcgaaatc gtcctcaaac
     6721 tcaaagacgt cttcgaagat ctttgcgccg acgttgtgat cttccatgat ctgactatcc
     6781 cgttgttgtt ggttggtgag ccagatctta ttcaagtcgt ccccccgggc tgtgacgcgg
     6841 ttcccagttt cgcgccatgg caggtacccg ctgacccggt catcgggcgg ttgcttacag
     6901 cgtcggggcg atctactgtt aggcggcggc aatctggggg gatactgaat gcggcacgta
     6961 ggggacatac tggccgagaa gtccaaagac atcgtctgga tcggcccgct cgaatccgtg
     7021 tacgacgcgc tcaagctcat gaacgaaaag gacgtgggcg ctgtgctcgt gcagatcgat
     7081 gagtatctcg tcgggatcct gtcagagcgg gactacatgc ggaaagtgat tctggacaat
     7141 catgcggcca agcagatcaa agtctccgag gtgatgaccc gggacgtggt cacggttacc
     7201 cgggaagaca ccgtcgtcca ttgcatcgcg ctgacgaaaa agcaccacgt ccgtcatctt
     7261 cccgtggtgg aggaaggaaa ggccatcggc atgatctcgc tgcgcgacct ctttctagac
     7321 gtcatccacg agaaggtggg tgaagtcggc gacgaagact gacagcggcg cgtccagcgg
     7381 ccgtccatcg tgtgaggagc gtcaggctga gcagctgccg ccgccgagga cgcagaactt
     7441 ggcgcagtgg ggatggttca gcgcgaccgg ccgcaggctg gaagccagcg tagggcccat
     7501 ggcgaagcgc tcgtcgtagg gcagcagcgt gcacgcgacc acctcggggg tttccgcccc
     7561 cctgcgcttg accaccattc gagagcttgc gcacatctga tcctccggcc gccgtcccag
     7621 cagatcccag cattcggtgg tgatctcggg cacatcggcg ctggtgtcca tctccgggaa
     7681 cagcaccagg ctcgcgggat catgcgcgtc gacggcgatc tcctcggcgg caaacagggc
     7741 ggcgaagccg gcccgaaggg tgtgctcgtc ctctccccag cgggtgcgcc cggcgatgtg
     7801 aacggcgaag ccggactcgc tcagccagcg taacccctcc atcatcggcc gccaggaacg
     7861 cgggcctctc tcctgtcggt ggcggtcagg ctcgaagtgg tcgactgaaa cccgcagcgt
     7921 cagagcgtcc ccgtagcggg agcccagggc cagcagcccg cgcgcgtgct tcatcatcgg
     7981 ccgcatggcg ttggtgagca ccagaacgcg atggcccgcc cccaggctgt cctcgagcat
     8041 gcccatgatg tcggggttca tgaatggctc gccgccggta aagccgatct cctcggtttc
     8101 gaggtcggag ccggcgatct cgtcgagata tgctcgaacc tcggcgcgag agatgtaggc
     8161 caggcggtcg ttggtcgggc tggactcgat gtagcagttc tcgcaggtca ggttgcacag
     8221 ggtgccggta ttgacccaca gggtcgtcag tccggttgga gccacggatg ccctgggctt
     8281 gccgtctgcc gtggtggtcg ggtcggcgaa cttcgcgtcc cgcgcgatga tctcggccat
     8341 ctctgattgc ccttgcgccg gcgtttgcgg cgctcgtcct gctcgatcgg tctctcatgc
     8401 tgacaggccg ccttctcccc cgcaacgggc gtacacgggt tcgtccggaa agacgagctt
     8461 tgtccgacac aagccggttt gccatactcg cgacctcttt tctgcagtcg acacaccggg
     8521 agcggcgata tgggacgagt tgagggcaaa gtggccatcg tgacgggcgg ggcgtccaat
     8581 cccggcctcg gccgcacgac ggcgctgacg ctggcgcgcg agggcgcgat cgtcgtggta
     8641 accgacgtgg atctggaagg tgccgaggcc accgcggcgg agataaggga tgcgggagga
     8701 agggcgctgg cgctgcacca ggacgtcacc gacgagggcc gctgggccga ggtcatcgcc
     8761 gctacggtgg atgctcacgg caggctggac gtcctggtca acaacgccgg gatcgcggtg
     8821 ctcatgcccc ttgccgagat gtctctggcg gactggaacc ggcagatcga ggtcaacctg
     8881 accagcgtct tccttggctg caagcacgcg gcaccggcca tgcgcgaggc gggtggcggg
     8941 tccatcgtca acctttcgtc ggtggctggt ctggtggggt tgccgacctg cgtcgcttac
     9001 ggggcggcca agggcggggt ccggatcatg tccaagagcg tcgccgtgga gctcgccgag
     9061 cacgacattc gctgcaactc ggtccacccc ggattcatct ggaccaacat gcaggctcag
     9121 tccgcgggtg cgtcagcccc cggcggtggc gcgatgcccc gggaccgggt gccgctggga
     9181 cgcatgggtg atccccagga catcgccaac tgcgttctct acctcgcctc cgacgagtcg
     9241 agctacgtca ccggcgccga gttcaccgtg gatgcgggta tgacggcgca gtagctggcc
     9301 ggctccgttc aggtcctccg gtaccgcgtt ggtttgagat cggcccgccg cagcgagcag
     9361 ggctgtcacg gaccgcgcca ctcacgcgca tccagcgcct ccccgctgat gccgttcgag
     9421 gcgtccgcca tcaggtagac gaacacgtcc atccgcgcct ctggcgtcgg cacgctggcg
     9481 gggttctcgg caggataggc ggcagcccgc atggccgtgc gcgtggcgcc cgggttcagg
     9541 ctgttcacgc ggatgatgcc ggcgtgctcg tgctcgtcgg ccagaacctc catcaggccc
     9601 tccagcgcgt gcttggaaac ggcgtaggct ccccagtagg cccggccctt gcgccccacc
     9661 gtggaggaga ccagcacgac cgacgcggct ggtgccgcgt tcagcagggg cagcagggtt
     9721 cgggtcagca ggaagggtgc ggtgacgttg acctgcagca cctcctgcca gtccgccgtt
     9781 ggaaacaggg cgatgggcac tttcgggccg agggccgagg cgcagtgcag gatgccgtcg
     9841 agccggccgt agccgtcggc gatggcgtcg tgcagggcgt cggcgctcgc gtcatccaaa
     9901 gctctgagat cgcaggggac gatcaccggc cgggtgtccg tctcggcctc gatccagtcg
     9961 aacacggttt ccagcttgga tcgcgtgcgc cccagcagca ccacgtcggc accgtagcag
    10021 gcgtacgttc tcgccgccgc cgcgccgatc ccgtcgccgg ctccggtgac caggatcacc
    10081 ttgcctcgca ggcagtcgtc ttccggctgg aaccgccagc cggcgcgcgc cgcctccagc
    10141 tcggggtact caggactttt cggcatagag catgtagttc acgtcgacgt cgggcgtcaa
    10201 cgagcactgc cgtgtgaacg ggttgtagcg cataccgatg atctctttca cggtcagccc
    10261 tgcggcgcga acggccgccg cgagctccgc ggggcggatg aacttgtcgt agtcgtgggt
    10321 gcctctgggc agcaggccca ggacgtactc ggcgcccagg atggccagca gatacgcctt
    10381 tggattgcgg ttgatggtgg agaaaaccag ttttccatcc ggtgctgcga ggtctgcgca
    10441 ggctttcacc gtggaagcgt agtcggggac gtgctcgagc atttccaggc aggccaccgt
    10501 gccgaacctg ccggcgcgct ccgcggccag gtcctcagcg gtggtagcca ggtactccac
    10561 ttgaacgccg ctgtcctccg cgtgcaggcg agccacggcc agggggctct ccgccatgtc
    10621 gatgcccgtc accgtagcgc cctcccgagc gaggctttcc gccagaatgc ccccgccgca
    10681 gcccacgtct accaccgggc gcgagcggat gtcgcagtgc tccatgagat agttgagccg
    10741 cacggggttg atgtcgtgga gcggcttgaa ttccccttcg ggatcccacc agcgcgcggc
    10801 cagggcgtcg aatttgccga tctccgcctg atccacgttc attgggatat tcgctcccgc
    10861 cacccgcgcg ccgcccgcag gatggcttgt tcgtccagcg tgcacagctg accgtcgcgc
    10921 agcagttgcc gccccgccac ccagacgtgg gccaccccgt ctccggacgt ggtgtggacc
    10981 agttgcgcga ccggatcgaa caggggctgg aatttcagcc cggtcaggtc gatggcgacc
    11041 atgtccgcgg ccttgcccgg ttccagcgaa ccgatctgcg cttccatgcc cagggctcgc
    11101 gcgccgccga gggtcgccat ctccagtgcc cgatgggccg gcagggcgcc cgcgtcgccg
    11161 tctctgagct tggccagcag cgttgccagg cgtgcttcca agagcaggtc gaggccgttg
    11221 ttcgacgcgg cgccgtcggt tcccagggcc acgttcaccc cccgggcgag cagggtcgtg
    11281 gtggcgcaga tgccgcttgc cagcttgagg ttcgacagcg ggcaatggac cacgtgcacg
    11341 ttgttctctg cgagcaggtc gatctcctcg tcgtccaggt gcgtggcgtg gacggcctgc
    11401 agattcggcc cgagcagccc gcgctcgtcg agatagcgca gccagctcat tccgacccgg
    11461 tcgcgagcct cggcgacctc ccgcgcggtc tcgtgaaggt ggatctgcac atgggcgtcg
    11521 agttcctcgg agtacacgag gatccgctcg aggtcgccct cggagacgct gtacgtggcg
    11581 tggggcccga acgcgacccg gatgagaggg tcgcgccggt agtcgtcgtg gagcgccagg
    11641 cccttgtgga acccctcgtc ggagcttgcg ctccacgcgt tgggaaactc gatgacggga
    11701 aaggcgatct gggcccgcat gccggccgcc cgcgcctccg cagccgtcac ctcgggaaag
    11761 aagtacatgt ccgagaagca ggtcgtgccg gtcttgagca tttcggccat ggccagccgg
    11821 gcgccgtcgc ggacgaagtc cgcatccacg agccgagcct cggtgggcca gatgtggtcg
    11881 ctcagccagt tctccagcgg cgcgtcctcc gcaaagccgc gcagcagcgt catggcggcg
    11941 tggccatggg cattgatcag ccccgggagc agcgcgtggc cgggcaggtt gtgcacctcc
    12001 cggggctggt gctcgccgtg aatcagctcg cgatcgccaa tcttcaggat cctgccgtcg
    12061 gcaatggcaa tgctcgcctc ctggagcacc gagcctgcag gcacgatggg cagtatccag
    12121 tcggcatcga tgatcagatc gcatggggtg gacggcatcg gccgattata gcccctccgg
    12181 ccgggcgtag gcgggttcat agagcgcgga gacgggcgga attccgtggt caaaagtatc
    12241 aggttcgcct gcgcttcgtg gtaaaatggc gcgcttttct gcagcggttc catatgtccg
    12301 acctggggtc cgatcagggc cgggagatcc tcccggtcaa catcgaagac gagcttcgac
    12361 agtcttatct cgattacgcc atgagcgtga tcgtcgggcg cgcccttccc gacgttcgtg
    12421 acggcctcaa gcccgtgcac aggcgggtgc tgttcacgat gagcgagctg aacaacacct
    12481 tcaaccggcc ctacatgaag tccgcccggg tggtaggtga ggtcatgggc aagtaccacc
    12541 cgcacggcga tgtcgccatc tacgacaccg tcgtccgact ggcccaggac ttctccatgc
    12601 gttacatgct cgtggacggc caggggaact tcggctccat cgacggggac ccgccggcag
    12661 ccatgaggta caccgaggtg cgcatggcgc gcatcgccgg ggagctgctg gccgacctcg
    12721 acaaggaaac ggtgagtttc gtaccgaatt acgacgacac cctcaccatg ccggaggtgc
    12781 tcccgacccg ggttccgaac ctgctcgtca acggcagctc cggtatcgcc gtgggcatgg
    12841 ccacgaacat accgccccac aatcttcgcg aggtcgtggc cgcctgcctg gcgctgctgg
    12901 acgatccgtc gctgaccgtc gacgacctca tggtgcacat ccacggtccg gactttccca
    12961 ctgccgccat catcaacggc cgggccggca tcgtgcaggc ttaccgaacc ggtcgcgggc
    13021 gcatctacgt tcgtgcgcgc gcggaagtga tcagtgacgg taagtccaac aaagacacca
    13081 tattgatcac cgagatcccc taccagctca acaaggcccg gctcatcgag aagatcgccg
    13141 agctggtcaa ggagaagaag atcgaaggga tcacggagct gcgggacgag tccgacaagg
    13201 acggcctgcg cgtcgtgatt gagctgcgcc gaggcgagcc gggcgaggtg gtgctgaaca
    13261 acctctacgc ccagacccag ctccaatcgg tgttcggcat caactgcgtg gccctggtag
    13321 acggccagcc tcgggtgctg aacctaaagc agatgttggg ggcgttcgtt cagcaccggc
    13381 gcgaagtggt cacccggcgg tcgatctacc tgctgcgtcg agcccgccag cgcggccaca
    13441 tcctggaagg ccaggccgtg gcgctggcca acatcgacgc cgtggtggag ctcatcaagg
    13501 cctcccggac gggtgcggac gcgcgtacgg cgctcatgag cagaggctgg atgtcggagg
    13561 cggtccagaa gctgctggag agagccggca gcgatgcctg tcggccggag ggcctgggcg
    13621 aagagttcgg ctttcgcgag gggctctact acctctccga ggagcaggcc caggcgatcc
    13681 tggagctcag gctgcagcgc ctcaccgccc tcgagcagga caagctcatc gaagactatc
    13741 aggtgattct cgacgagatt gccgatcttc aggacattct cggctccgaa gagcggctcg
    13801 tctcggtcat tcgagcggaa ctcgaggaga ttcgggacac ctacggcgac gagcgcagaa
    13861 cggagattct ctcctcccac gaggacctct cggccgagga tctgatcacc gaggaagacc
    13921 tggtggtcac catctcccat cagggctacg cgaaaaccca gtcgctggac acttatcagg
    13981 cgcagcggcg cggcgggcgc ggcaaggcgg ccacggcggt gaaggacgag gacttcgtgg
    14041 agcacctgct catcgccaac agccacgcca cgctgctgtg cttttcgaat ctgggcaagg
    14101 tctactggct gaaggcctat cagattcccc gcgcgagccg gggcgccaag ggccggccgc
    14161 tggtgaacat gctgccgctg gcggcggatg agcggatcac ggtggttctt cccatcgagg
    14221 atttcgcgga ggatcgcttc gttttcatgg ccaccgccaa cgggacggtg aagaagacgc
    14281 cgctggagca gtttgcccgc cagcgctcca ccgggctcat cgcgttggag ctggaggaag
    14341 gtaacaccct cgttggcgcc gcgttcacgg acggtcactg cgacgtcctg ctcgtttcga
    14401 gctccgggaa agccgtgcgc ttcaaggaat cggacgttcg cgcaatgggc cgcaccgcca
    14461 ggggcgttcg cgggatccgc ctggcgggtg atcatcgggt gatctccctc atcattccgg
    14521 ccccggacgg attgatcttg agcgcgagcg agaacggcta cggcaagcgc acggtggtgg
    14581 acgagtttcc ggtgaaagga cgtggcggcc agggcgtcat cgccatgcag cgcagcgacc
    14641 gcaacggcga cgtggtgggt gtcgtccagg tcttcggcgg agacgagctg atgctcatca
    14701 gcaacatggg cacgctggtg cgaactgcgg cggacgaggt gtccgtgcag gggcgcaata
    14761 cccagggggt tcgcctgatc aacctgcgcg gcgacgagaa gctcgtggga gtggagcgaa
    14821 tcgtcgattc ggagagcgct gaggcggacg aagaaagcgc cgacgcagac gaagaatgaa
    14881 ccgcgcgatc aacttctcgg ccggcccggc ggcgctgccc acacaggtgc tgcagcaggc
    14941 ccaggccgaa ctgctggact acgccggcac gggcatgtcg gtcatggaga tgagccaccg
    15001 cagcgcggag ttcatcgaca ttgccgagcg cgccgagcgg gatttcagag aactcctgca
    15061 gatcagcgac gactatcacg tgctgtttct gcagggaggc gccacgctgc agttcgccat
    15121 ggtgcccctg aacctgctgg gcgatcgtgg cacggcggat tacgtcaaca cgggctcctg
    15181 gtcgaccaag gcgatcaagg aagccgcccg cctgtgctcg gtcaacgtgg ttgcgagtgc
    15241 cgaggagcac ggtttcgacc ggattccgga tcctgctgcg tgggcctgca gcgctgatgc
    15301 cgcctacctc catatatgct cgaacgagac catcggaggg gttcagttcc acagctttcc
    15361 cgaccgcgcc ggcggcccgc cgctggtggc agacatgtcg tcggacatcc tgtcccggcc
    15421 catcgacgtc agccgtttcg gtctcatcta cgccggcgcc cagaagaacg tcgggccggc
    15481 gggactgacg ctcgtcgtcg tgcgcaagga tctggttggg gccgcgcggc ccggcacgcc
    15541 cagcatgctg gactacgccg ttcacgccga ggcggggtcc atgtcaaaca ccccgccgac
    15601 ctacgcctgg tacctggcgg ggctggtgtt cgcctggatc aggtccctgg gcggggtcgc
    15661 cgccctcgat gagctcaacc gccgcaaggc gggcaagctc tacgcggcca tcgacggcag
    15721 caatctgttc cgcgcgccgg tggtggttga agaccgatcc atcatgaacg tgccgttcac
    15781 gctccccgac ggcgagctgg atgctgcatt cctggccggc gccaaggacc gcggcctgct
    15841 caatctgaag ggacaccgaa gcgtgggcgg gatgcgggcg agcctttaca acgcggtgcc
    15901 cgaatccgcg gtggatgcgc tgatctcgta catgcaggaa ttcgagaccg atcacttcag
    15961 atgagcaccc gggacgacag gctcaaagcg gtccgcgacc gtatcgacgc catcgaccgg
    16021 gagctccaga aactcctgaa cgagcgggct gcctgcgcgc tggaggtggg tgagatcaag
    16081 caggcggacc cggatgcgga ggcgccggtg ttctatcggc cggagcggga agcccagatc
    16141 ctttcgcggc tcaagcagga gaaccagggg ccgctgagcg acgacgacgt cgcgcgcctt
    16201 ttccgcgaga tcatttcctg ctgtctgaat ctcgagcagc ctctgaccat cgcttacctc
    16261 gggccggcgg gaacctacac cgaagccgcc accgtcaagc agttcgggca ctttgcggga
    16321 acggcggcgc tcgcatccat cgacgaagtg tttcgcgagg tggaaactgg cggcgcccat
    16381 tacggcgtgg tgccggtgga gaactccacg gaaggcatgg tgaaccacac gctcgactgc
    16441 ttcatcgact cgccgctgaa aatctgcgcg gaggtggagc tgcccatcca tcacgccttc
    16501 atggtggcgc cggacgccag cggcgagggc atcgaggaga ttctgtccca ctcccagtcg
    16561 ctcgctcagt gccgcacctg gctggacgcg cactatcccg gcgttccccg cacgcccgtc
    16621 aacagcaatg ccgaggcagc gaggctggtt gccgcccgta ccaatgctgc cgccgttgcg
    16681 ggcgacatgg cggccgaacg ttacggactg cgggtcatcg cctcccacat cgaggaccag
    16741 acggacaaca agacgcgctt tctggtcatc ggccagcagc gcgtgggtcc gagcggtcgt
    16801 gacaagacct ccatcctggt ttccacccgc aacgagcccg gcgcgctcta caagattctc
    16861 gagcccttcc atcgacacgg catcagcctg tcgcgtatcg agacgcggcc ggccagatcg
    16921 ggcagctggt cctacgtctt cttcatcgat ttcgacgggc atggcagcga gccggcaatc
    16981 gaagccgtca tgcgggaggt cggcgacgtg gccatggagg tccgttcgct gggctcctat
    17041 ccccaggcgg tggtctgaat gtcgccgcgc gttaacccca ggatcaaggc gctcaagccg
    17101 tatcagcccg gcaagccggt agaagaagtc acccgggaac tcgggctggt cgacgtgatc
    17161 aagctggcga gcaacgagaa ccctcgcggg cccggcgagc tggtgcggca ggccatcgat
    17221 gcggcctgtg gagaccttgc ccgctacccg gacggcaacg cgttcgcgct caagcaggcg
    17281 ctggcccggc atttcggggt gcgggagagc cagctcaccg ttggcaacgg ctccaacgac
    17341 gtcctcgatc tggccgcgcg catgagcctg tccccggggg ccgaagcggt gatctcggaa
    17401 cacgccttcg tcgtctaccg cctggccacc ctgtccgcgg gtggcgagct cgtcgaggtg
    17461 ccggctcgga actacggcgc ggatctcgac ggaattctgg atgccatcac cgacgccacg
    17521 gccatcgtct ttctcgccaa ccccaacaac cccaccggca cctgggtggg cgaaggggcg
    17581 ttggtgcggt tcttggacga ggttcccgag cacgtctggg tcgttctgga cgaggcctat
    17641 cacgaatacg tggaggcggc cggataccct aacggcatgg agcttcagcg gcgctatccg
    17701 aacctgatcg tcacgcgcac gttctcgaag attcacgggc ttgccgccct tcgagtgggc
    17761 ttcagcgtga gctccgagga agcggccgac ctgatgaacc gggcccgcca acccttca
//
LOCUS       DBNHLM010000364         7808 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_535506, whole genome shotgun sequence.
ACCESSION   DBNHLM010000364 DBNHLM010000000
VERSION     DBNHLM010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7808)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_535506"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="AC16QF_16945"
     CDS             <1..267
                     /locus_tag="AC16QF_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6450298.1"
                     /translation="EALIARTFVDQCVLKLVNGELDAVTASMAKYWCTQKQCEIIDEC
                     LQLHGGAGYMDEYEISRMYADARVQTIYGGTNEIMKELIGRSLG"
     gene            275..1555
                     /locus_tag="AC16QF_16950"
     CDS             275..1555
                     /locus_tag="AC16QF_16950"
                     /inference="COORDINATES: protein motif:HMM:NF018913.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450299.1"
                     /translation="MIRYWRFYWPLALTGLAMVLAVQFQNAALARFPEAVTELAVFAL
                     AYSAYALFHASLNFVAQLSNVYARSPQGTSRSWRFATAASTCIMLPLLALAHTDYGAD
                     ALGSIYGIDDGLVERVTEYLAYLAPLLLISAQRFYYNGLLVQARLTGWVTTLTVVYLT
                     SVLVGLIIGFTLGLKPVHVLVGAEGIALLIQLALSVLIKASRYAPPADPEHADLSYGE
                     LLRFYLPVSTTAVMFALSRPILYAFVARTPNGVLAIAAMRVAFDFATIFQQAANQFRH
                     FFVTFGWNDLGIKRRFLTVICAGITAIMLAVALTPLSELIWGKLMGIPEPVRDIAEHV
                     LVVMCLMPALIVFRNYYHGRLMVERRTSGMAAGGILRVIAILLAAQACTMMGWLNYLS
                     ASFILILGFLVEALVALVAAQKGQGLRANATANM"
     gene            complement(1510..1869)
                     /locus_tag="AC16QF_16955"
     CDS             complement(1510..1869)
                     /locus_tag="AC16QF_16955"
                     /inference="COORDINATES: protein motif:HMM:NF016161.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HIR6450300.1"
                     /translation="MLKSIHLILAYLTVLGFVVRGIWSLVDSPLKNQKWVRISPHVID
                     TALLVLGVALAVQLSVSPLEGWLAAKLAGLLAYIGFGVLTMRASSTPLKLAGFAGALA
                     SVGYIFAVAFARSPWPF"
     gene            1957..3420
                     /locus_tag="AC16QF_16960"
     CDS             1957..3420
                     /locus_tag="AC16QF_16960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR6450301.1"
                     /translation="MNIDQAIDQAMRPLADAVSGFIFFSVPVLGTQIPLIVVWLILGG
                     LFFTVYLRFVNIRGFAHAIGLVSGRYHNDQHPGEISQFQALTTAVSGTVGIGNIAGVA
                     VAISMGGPGATLWLIIAGLLGMSTKFAECTLGLKFRRHHEDGSVSGGPMYYLERGLAE
                     RNWPRVGRGLGLFYAASMVIGCLGIGNMFQSNQAAAIFIDVSGGDASYFQDKAWLLGL
                     IMAGAVGLVIIGGIRSIARTTSRLVPGMALLYVISALAIIAINAAELPAAFAAIWAGA
                     FSPEGIAGGLIGVMIIGFRRAVFSNEAGLGSAAIAHSTAKTERPVSEGYVALLEPFID
                     TVVICTMTALVIITTVYEPGLTSGNVSGIELTTRAFASTLSWSPIPLSIAAILFAFST
                     MIAWSYYGLKAFTYLVGHHRVADLGFKIFFLVFVVLGSSVQLGALVDLSDALVFVVAI
                     PNLLGLYLLAPTVRAELRAYESNMPVPNGAAPAPDST"
     gene            3475..4344
                     /locus_tag="AC16QF_16965"
     CDS             3475..4344
                     /locus_tag="AC16QF_16965"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6450302.1"
                     /translation="MEGPTSHVYFSQRLRLHYVDWGNPDAPPMLMIHGGRDHCRNWDW
                     VARSLRDRYHIIAPDLRGHGDSQWMLGGSYNQIDYVYDIAQLLTQKDMTPVTIIGHSL
                     GGSIALTYSGLFPETVKKLVAIEGMGPPPEFIKERLAVPVADRLHQWISDLRELSGRI
                     PRRYATLEEAYERMQTENPHLSEEQARHLTIHGSNQNEDGTYSWKFDNYVRAFPPIGI
                     DFEEQYKLHGRITCPTLLIRGTESWASDPSEDGRAARFQNATVRNIERAGHWVHHDQL
                     DEFLEIVDGFLAD"
     gene            4347..5408
                     /locus_tag="AC16QF_16970"
     CDS             4347..5408
                     /locus_tag="AC16QF_16970"
                     /inference="COORDINATES: protein motif:HMM:NF013621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450303.1"
                     /translation="MSQRAVILHRSPWQGVFHVTGGGSGLLAELLTTPGASGTVLEAV
                     VPYAQAALADLLGRAPDQACSPSTARSLAMSAFDRARDLGAREPFGLGCTASLATERM
                     KRGKHRACIAVQTTTSTYSATLQLEGDRASEERDLVELIWHALSRTLELHLESAPPDT
                     FDGGLTSALEHWRALVLGEETAHATAPHDGRLLLPGSFNPLHRAHRRMLELAEHLTGL
                     EGAYELSIVNVDKPLLDYTEIEERLSQFEAPVWLTRLPTFVDKARYFPGAHFAVGVDT
                     IIRITEAGYYGSQADRDAALDELATLGTRFLVFGRILNDAFLTLSDLDLPATFRALCR
                     EVTEAEFNEPISSTEIRRR"
     gene            complement(5423..6313)
                     /locus_tag="AC16QF_16975"
     CDS             complement(5423..6313)
                     /locus_tag="AC16QF_16975"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF018533.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fpg/Nei family DNA glycosylase"
                     /protein_id="HIR6450304.1"
                     /translation="MPELPDLTVYVEHLERRLVGQPLEAIRIASPFVLRSVEPTVDEV
                     CGRRILGCHRLAKQLVMELEGEHFVVVHLMIAGRLKWQKPSAPLPKRNALAAFDVPAG
                     SLLLTEASSKKRASLRLVRGAQALGALNPGGLEVLSTDLAAFASRLRESNHTLKRALT
                     DQHVFAGIGNAYSDEILLRARLSPFKRPRDMADGEIQRLHEATKGVLGEWTDRLRAAA
                     GDDFPSKVTAFHPEMSVHGKYRQPCPVCGAPVQRIVYAENEANYCARCQTGGRLLADR
                     SLSRLLKENWPKRVEELEGG"
     gene            complement(6306..7685)
                     /locus_tag="AC16QF_16980"
     CDS             complement(6306..7685)
                     /locus_tag="AC16QF_16980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR6450305.1"
                     /translation="MSEGGQAGELQGRRPGIWELAFPSILSNLSFTLVGLVQTKFVGE
                     LGAQALAAVGAGQRVFFAMQAILMAVSAGTTALVARAWGAGDYVEASRVTMASLVLAG
                     AFSVVVAVIGLFFAPVIAGVFGLDPETLGMASENIRWLCVFNVAFAVNFILGAALRAS
                     GDAWSPLWISGAVNVVNVPLLYVFIFGEFGFPRMGVAGAAIAAGIAFSIGGAVLLGLW
                     VKQKFRVKHVASGWWRKARLRQLLDIGYPAAVEQGVFQLGFFIFLMLIGNFYGTEAFA
                     AYNIGANMLMVCMTVGFGFSIAGATLVGQHLGANDPDGAARSGWRSTGLAVLAMGGLG
                     GVVCFYAEELAVFFIGDEPLTVDYTVQFTYILGAMMPLLAVEFTIGGALRGAGDTRFP
                     LMATFLGLIGMRCGLAALATYLGVPVFWVYAALIGDYLLKGVLLISRFRGGRWKTAIR
                     YREPEALGA"
BASE COUNT         1394 a         2657 c         2415 g         1342 t
ORIGIN      
        1 gaggccctca tcgcccgcac cttcgtggac cagtgcgtgc tcaagctcgt caacggcgag
       61 ctcgacgccg tcacggcatc catggccaag tactggtgca cccagaagca gtgcgaaatc
      121 atcgacgagt gcctgcagct gcacggcggt gccggctaca tggacgagta cgagatctcg
      181 cggatgtacg ccgatgcaag agtgcagacc atttacggcg gcaccaacga gatcatgaag
      241 gagctcatcg gccgcagcct gggatagttc caggatgatc cggtactggc gcttctactg
      301 gcctctcgcc ctcaccggcc tggccatggt actggccgtg cagttccaga acgcggcgct
      361 cgcccgtttc ccggaagccg ttaccgagct ggccgtgttc gcgctggcat acagcgcgta
      421 cgcccttttc cacgccagcc tgaacttcgt cgcgcaactc tcgaacgtct acgctcgcag
      481 cccccagggc acctcacgca gctggcgatt cgccaccgct gcgagcacgt gcatcatgct
      541 gccgctgctc gcgctcgctc acaccgacta cggcgccgac gcgctgggct cgatctacgg
      601 catcgacgac gggctcgtcg agcgggtcac ggaatacctc gcctacctcg ctcctctcct
      661 gctgatcagc gcgcagcgct tctactacaa cggtctgctc gtgcaggccc gattgaccgg
      721 ctgggtgacc acgctgaccg tcgtctacct caccagcgtg ctggtcgggc tgatcatcgg
      781 cttcacgctg ggactcaagc ccgtgcacgt tctggtcggc gcggagggca tcgccctgct
      841 gatccagctg gcgctgtccg tgctgatcaa agcaagccgc tacgcccctc cggcagaccc
      901 ggagcacgcg gacctgtcct acggcgagct gcttcggttt tatctcccgg tgtcgaccac
      961 ggcggtcatg ttcgcgctgt cacggcccat cctctacgcg ttcgtggcac gcacgcccaa
     1021 cggagtgctc gccatcgccg ccatgcgcgt agcgttcgac ttcgccacca tttttcagca
     1081 ggccgccaac cagtttcgcc acttcttcgt gaccttcggt tggaacgacc tgggaatcaa
     1141 gcgccgcttc ctcaccgtga tctgcgcggg tatcaccgcg atcatgctgg ccgtggcgct
     1201 cacacccctg tccgagctca tctggggcaa gctgatgggc atcccggaac cggtccgcga
     1261 catagccgag catgtgcttg tggtgatgtg cctgatgcca gctctgatcg tgttccggaa
     1321 ctactatcac ggccggctga tggtcgagcg ccggacgtcg ggcatggccg ctggcggcat
     1381 tctcagagtc atcgcgatac ttcttgcggc gcaggcatgc acgatgatgg gctggctgaa
     1441 ctacctgagc gcgtcgttca tcctgattct gggctttctg gtggaagcgc tggtggcgct
     1501 cgtagcggct cagaaaggcc agggactgcg ggcaaatgcc accgcgaata tgtagcctac
     1561 gcttgccagg gcaccagcga acccagcgag cttcagcggc gtcgagctcg cccgcatcgt
     1621 gaggactccg aatccgatgt aggccaggag cccggcgagc tttgctgcca gccagccttc
     1681 cagtggcgaa acgctgagct gcacggccag cgccacaccg agcacgagca gcgccgtatc
     1741 gatgacgtgc ggcgagatac gcacccactt ctggttcttc agcggagagt cgaccaggga
     1801 ccagattccc ctgaccacaa acccgagcac cgtcaggtag gcaagaatca gatggatcga
     1861 tttcagcatt gtctctcccc gggcggtcct cgatcacttc gtttatgatc ggcctcccag
     1921 cgcattcaag caacccgaca cctcggaaaa ctatctatga atattgatca ggccatcgac
     1981 caggccatgc ggccccttgc ggacgcggtg tccggattca tcttcttctc tgtgcccgtt
     2041 ctcggaaccc agatcccgct gatcgtggtc tggcttatcc tcggggggct tttcttcaca
     2101 gtgtatctgc gcttcgtcaa cattcgcggc ttcgcccacg ccatcgggct ggtcagcggg
     2161 cgctaccaca acgaccaaca cccgggtgag atctcgcagt tccaggcttt gacgactgcg
     2221 gtatcgggca cggtgggaat aggcaacatc gccggggtag ccgtggccat ttccatgggc
     2281 gggcccggcg cgacgctgtg gctgatcatc gccggcctgc tgggcatgtc gacgaagttc
     2341 gccgaatgca ccctcgggct caagtttcgg cggcatcacg aggacggctc ggtttccgga
     2401 ggcccgatgt attatctcga gcgcggcctg gcggagcgca actggccgcg ggtcgggcgc
     2461 gggctcgggc tgttctacgc ggcatccatg gtcatcggct gtctcggtat cggcaacatg
     2521 tttcagtcga accaggcggc agccatcttc atagacgtca gcggcggcga tgcgagctac
     2581 ttccaggaca aggcttggct actgggcctg atcatggccg gcgcggtagg gctggtaatc
     2641 atcggcggca tccgttccat tgcgcgcacc acctcgaggc tggtccccgg tatggcgctg
     2701 ctgtacgtga tctcggcgct tgccatcatc gccatcaacg cggccgaact gcctgccgcc
     2761 ttcgccgcca tctgggcggg cgccttctct ccggagggta tagccggagg cctcatcggc
     2821 gtcatgatca tcggtttccg cagagccgtg ttctccaacg aggccgggct cggctccgct
     2881 gccatcgccc actccacggc gaagaccgaa cgcccggtga gcgaaggcta cgtcgctctc
     2941 ctggagccct tcatcgacac cgtcgtgatc tgcaccatga ccgccctggt catcatcacg
     3001 acggtgtacg aacccgggct gaccagcggc aacgtgagcg ggatcgagct caccacccgc
     3061 gcgtttgcga gcacgctgtc atggtcgccg ataccactgt ccatcgccgc catcctcttc
     3121 gcgttctcca ccatgattgc ctggtcctac tacggcctga aggccttcac ctatctggtg
     3181 ggccaccacc gggtcgccga cctgggcttc aagatcttct tcctcgtctt cgtggttctg
     3241 ggcagcagcg ttcagctggg tgccctggtg gacctctccg acgcgctcgt gttcgtggtc
     3301 gcgatcccca acctgctggg gctgtacctg cttgccccca cagtgcgggc cgagctgagg
     3361 gcctatgaat ccaacatgcc ggtgcccaac ggcgcggcgc cggctcccga cagcacctga
     3421 gccgggcgcg ttataatgcg cgagctctca gcacgccgga acgctttatc gacaatggaa
     3481 ggacccacct cccacgttta cttctcccag cggctcaggc tgcattacgt ggattggggc
     3541 aaccccgacg ctccacccat gctgatgatt cacggcggcc gcgatcactg caggaactgg
     3601 gattgggtgg cccggtcact tcgcgaccgc taccacatca tcgcccccga cctgcgcggt
     3661 cacggcgact ctcagtggat gctcggcggg agctacaacc agatcgacta cgtgtacgac
     3721 atcgcccagc tgctgactca gaaggacatg acgccggtga cgatcatcgg gcactcgctg
     3781 ggtggctcca tcgcgctcac ttactcgggt ctgttcccgg agaccgtgaa gaagctggta
     3841 gccatcgagg gcatggggcc gccgcccgag ttcatcaagg aacggctcgc cgtaccagtg
     3901 gcggaccggc tgcaccagtg gatctccgat ctgcgcgagc tttcgggaag gatcccccgt
     3961 cgctacgcga ccctggagga agcctacgag cgcatgcaga cggaaaatcc tcacttgagc
     4021 gaggagcagg cacggcacct gacgatacat ggcagcaacc agaacgagga cggcacctac
     4081 agctggaagt tcgacaacta cgtacgggct ttcccgccca tcggcatcga cttcgaggag
     4141 cagtacaagc tccatggccg catcacctgc cccaccctgc tgattcgcgg caccgagtcc
     4201 tgggcgtccg accccagcga ggacggccgc gccgcgcgct tccagaacgc caccgtgcgc
     4261 aacatcgaaa gggccggaca ctgggtccac cacgatcagc tcgacgagtt tctggaaatc
     4321 gtggacggtt ttctcgccga ctgagaatga gtcagcgcgc ggtcattctg caccgctccc
     4381 cctggcaggg cgtgttccac gtaaccggcg gcggatcggg tctgctggcc gagctgctga
     4441 ccacgcccgg cgcctcgggt accgtcctgg aggcggtggt tccgtacgcc caggcagcac
     4501 tggccgacct cctcggccgc gcgcccgacc aggcctgctc gccgtccacc gcccgctccc
     4561 ttgccatgag cgccttcgac cgcgcccggg atctcggggc ccgcgagccc ttcgggctcg
     4621 gttgcacggc gagcctggca accgaacgga tgaagcgcgg gaaacaccgc gcctgcatag
     4681 ccgtgcagac gacgaccagc acctacagcg ccacgctgca gctggaaggc gaccgggcca
     4741 gcgaggagcg cgatctggtg gagctcatct ggcacgcgct cagccggacg ctagagctgc
     4801 acctggagtc ggcaccgccc gatactttcg acggcggtct gaccagcgcg ctggaacact
     4861 ggcgcgcgct ggtgctcggt gaggagaccg cccacgccac cgcaccgcac gacggccgtc
     4921 tgctgctgcc cggctcgttc aatccgctcc atcgggcgca ccgccggatg ctcgagctgg
     4981 ccgagcatct caccggcctc gagggggcat acgagctgtc gatcgtgaac gtggacaaac
     5041 cgctgctgga ctacacggaa atcgaggagc ggctcagcca gttcgaggcg cccgtctggc
     5101 tgacccgact gccgactttc gtcgacaagg cccgttactt cccgggcgct cattttgccg
     5161 tcggcgtgga cacgatcatc cgcatcaccg aagccggcta ctacggcagc caggccgacc
     5221 gggacgcggc ccttgacgag cttgccactc tcggtacgcg ctttctcgtc ttcggcagaa
     5281 ttctgaacga cgccttcctg acgctctccg atctggacct gccggcgacg ttccgggcgc
     5341 tgtgccgcga ggtgaccgag gcggagttca acgagccgat ctcctcaacg gagatccggc
     5401 gacgctagcc caacggggcc gctcagccgc cctcgagctc ttccacccgc ttcggccagt
     5461 tctccttgag caggcgggac agggaccggt ccgccagcag gcgcccgccc gtctggcatc
     5521 gagcgcagta gttggcctcg ttctcggcat acacgatgcg ctgcacggga gcgccgcaaa
     5581 cgggacacgg ctggcgatac tttccgtgca cggacatctc cggatggaaa gcggtcacct
     5641 tcgacgggaa gtcgtccccg gccgccgccc ggagacggtc cgtccactcg ccaagcacgc
     5701 ccttggttgc ctcatggagc cgctggatct caccgtccgc catgtcccgc gggcgcttga
     5761 acggcgacag ccttgcccgc agcaggatct cgtcggagta ggcgttgccg atgcccgcaa
     5821 agacgtgctg atccgtcaga gcccgcttca gcgtgtggtt ggattcgcgc agtcgagacg
     5881 caaaggcggc aagatcggtc gagaggacct cgagaccgcc cggattcagc gctccaaggg
     5941 cctgggcgcc gcgcaccagc cgcagcgacg cccgcttctt gctgctggct tccgtgagca
     6001 gcaggctgcc cgcgggaacg tcgaaggcag ccagcgcgtt gcgttttggc aggggcgcgc
     6061 tcggcttctg ccacttcaga cggcccgcga tcatgaggtg aacgaccacg aagtgctcgc
     6121 cctccagctc catgaccagc tgcttggcga gccgatggca acccaagata cgccggccgc
     6181 agacctcgtc caccgtcggt tcgaccgagc ggagcacgaa agggctggca atgcggatgg
     6241 cctcgagcgg ctggcccacc agtcgcctct cgagatgctc gacgtagacg gtcaggtcgg
     6301 gcagctcagg caccgagggc ctccggttcc cggtagcgga tggccgtttt ccagcggccg
     6361 ccgcggaacc gcgagatgag caggacgccc ttgagcaggt agtcgccgat cagtgctgcg
     6421 tacacccaga acacgggcac gccgagatag gtggccaggg ccgcgagccc gcagcgcatg
     6481 ccgatgagcc cgagaaaggt cgccatgagc gggaagcggg tgtcgccagc acccctgagc
     6541 gcgccgccga tggtgaactc gacggccagc agcggcatca tggcgccgag gatgtaggtg
     6601 aactgcaccg tgtagtccac ggtaaggggc tcgtcaccta tgaaaaagac ggccagctcc
     6661 tctgcataga agcagacgac cccccccaag ccacccatgg cgagcaccgc aaggcccgtg
     6721 gagcgccagc cgctgcgggc ggcgccgtcc ggatcgttcg cgccgaggtg ctggcccacc
     6781 agcgtcgcgc cggcgatgga gaagccgaac ccgacggtca tgcacaccat gagcatgttg
     6841 gcgccgatgt tgtaggcggc gaaagcttcg gtaccgtaga agtttccgat cagcatcagg
     6901 aagatgaaga agccgagctg gaacaccccc tgctcgaccg ctgccgggta gccgatgtcg
     6961 agaagctggc gcaaccgcgc cttgcgccac cagccgctcg ccacgtgctt gacccggaac
     7021 ttctgtttga cccagagccc gagaaggacg gccccgccga tggagaacgc gatgccggcc
     7081 gcaatggctg ctccggcaac ccccatgcgg ggaaaaccga actcgccgaa gatgaacacg
     7141 tagagcagcg gaacgttgac gacgttgacc gcgccgctga tccaaagagg cgaccaggca
     7201 tctccagatg cgcgcagcgc ggcgcccagg atgaagttga cggcgaaggc gacgttgaaa
     7261 acacacagcc agcggatgtt ctctgaggcc atgccaagcg tttccggatc gagcccgaag
     7321 acgccggcaa tgacaggggc gaaaaacaga ccgatcaccg cgaccacgac cgagaaggcg
     7381 ccggcgagaa ccagcgaggc catggtgacg cggctcgcct cgacgtagtc cccggcgccc
     7441 caggctctgg ccactagagc cgtggtgccc gcgctgaccg ccatcagaat ggcctgcatg
     7501 gcaaagaaga cacgctgccc tgcgcccacg gccgcgagcg cctgggcacc cagctcgccg
     7561 acgaacttgg tctgcacaag accaacgagg gtgaatgaca ggttgctcag gatggacgga
     7621 aacgccagtt cccagatgcc cggtcgccgt ccctgaagtt ctcctgcctg accgccttcg
     7681 gacatctgcc tccttctcga acgcaccagg ttgcttatgg aatgcccgcc gcgctggctg
     7741 ctaatatcct ccgctcgctc gaattcagca aacggggata aggatcttga ctgaatacac
     7801 cgacatcg
//
LOCUS       DBNHLM010000365        12774 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_415775, whole genome shotgun sequence.
ACCESSION   DBNHLM010000365 DBNHLM010000000
VERSION     DBNHLM010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12774)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12774)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12774
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_415775"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..558
                     /locus_tag="AC16QF_16985"
     CDS             25..558
                     /locus_tag="AC16QF_16985"
                     /inference="COORDINATES: protein motif:HMM:NF016064.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HIR6450306.1"
                     /translation="MNTLIIGFLIGCAFGAILYMGGASSYRRILGTLLLKDMWIIKLM
                     ATAIGVGTLGVYLLDLGGLAHMSIKPAYIWGIALGGIIFGIGWAVSGYCPGTCVVGTS
                     EGKRDALFTLLGAFVGAFVFSLVFPYLESGLIERANFGSITLEDVLGFNGIYLAIPFS
                     LALIALAFFGLRDRYGQ"
     gene            complement(595..1068)
                     /locus_tag="AC16QF_16990"
     CDS             complement(595..1068)
                     /locus_tag="AC16QF_16990"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR6450307.1"
                     /translation="MQRSNPIPQAGLLALASLLASLPWADALAEGMNDEKPFAEAHVI
                     LQVSDADPSRHQTVLDIANNLTRHYGGPDMADIEIIAFSSGVKILLTTDNPHTQRIAN
                     LMENGVRFYVCGNTLDTIERRDGKRPVVLPGVREVQTGVAFMIEEIAKGYVPVHP"
     gene            complement(1093..2841)
                     /gene="soxB"
                     /locus_tag="AC16QF_16995"
     CDS             complement(1093..2841)
                     /gene="soxB"
                     /locus_tag="AC16QF_16995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HIR6450308.1"
                     /translation="MNRREFLEILGAASITAQLSGCTSLRTARPDDYYALPAWGDARL
                     LHITDTHAQLNPIYFREPSINLGVGAARGQLPHLVGRNLLDVLTSTNPRDVHAFTCID
                     FSEAAERYGRVGGFAYVKTLIDTLRGESGALLLDGGDTWQGSGTSLWTRGRDMVEACN
                     LLGVDVMTGHWEFTYQAQEVLDNADLLSADFVSQNVRATEDSLFEGTHVFDEDTGHVF
                     PPYVVKELNGRRIAIIGQSFPYTPIANPQRFIPDWTFGIRESDLAELVAQVRSRERVD
                     AVVLLSHNGMDVDLKLAGRVPGIDFILGGHTHDGVPVPAVVANPGGRTVVTNAGSNGK
                     FVGVLDIKFGAAGIDGYRYRLLPVFANLLEPDPQMQAHIDRVREPYLQRLEEPLAVTD
                     SLLYRRGNFNGTFDQVICDALRQVLDAQISLSPGFRWGTSVLPGQTITMENVMDQTGM
                     TYPETYVREMRGEEIRVILESVADNLFNPDPYYQQGGDMVRVGGLRYSCAPDAPNGER
                     IRNLELTDGTPLQAGAAYKVAGWATVGERSPGPPVWDVVADYLRDQGTIRIDKANEPT
                     LEGVKDDPGIARYDCS"
     gene            complement(2838..3236)
                     /locus_tag="AC16QF_17000"
     CDS             complement(2838..3236)
                     /locus_tag="AC16QF_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450309.1"
                     /translation="MINYLSKIGVLSLVGILCAGCAEPGQESVADATDGPAAVQRAEP
                     PEGLDPAARATWLIEAAQAAYADSQAAGHAWVTASRALEDARTAAARGDFASAGAQGE
                     KALRLAEASLAQARTEEGAWQDRFPKPPAQ"
     gene            complement(3233..3979)
                     /gene="soxA"
                     /locus_tag="AC16QF_17005"
     CDS             complement(3233..3979)
                     /gene="soxA"
                     /locus_tag="AC16QF_17005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HIR6450310.1"
                     /translation="MALRDYYAGRFPQIALEDHRDGVYAIDAGAREQWMEMEEFPPYE
                     FAVDEGAELFSTPFTSGAGYSDCFGDEGDVAGQFPHFSEEEATVVTLPMAVNACRDSH
                     GEAPLAYEGAEMARLTAYIAYASRGQQINVPEPASAAAAAAYQAGKRFYATRRGQLNF
                     ACTSCHVQLAGNRLRAERLSASLGHVTHWPVYRFKWEDVGSLHKRFQECNSQVGAEPF
                     DEQSEPYRNLEYFLAYMSNGLELNGPATRK"
     gene            complement(4096..4407)
                     /gene="soxZ"
                     /locus_tag="AC16QF_17010"
     CDS             complement(4096..4407)
                     /gene="soxZ"
                     /locus_tag="AC16QF_17010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HIR6450311.1"
                     /translation="MANDVKIRGKITDGVATVKSLMVHPMETGTRRDADGNLIPAHYI
                     EKVAAYKNDQEVLTADWGASISKNPFLSFRVKNAAPGDVIRITWVDNTGATGAGEAVL
                     K"
     gene            complement(4432..4878)
                     /gene="soxY"
                     /locus_tag="AC16QF_17015"
     CDS             complement(4432..4878)
                     /gene="soxY"
                     /locus_tag="AC16QF_17015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HIR6450312.1"
                     /translation="MALNRRKWITSMATAGAAVLLPLRLLADTAAAFKAADANTALKQ
                     LFGDLPVEESTAIEFKIPDIAENGAVVPVTVSTDMPGVRNISIVVDENPNPLSASFNI
                     SPEAFADVSTRVKMGNSSVVHAYVRTADKVYTTSKEVKVTIGGCGG"
     gene            complement(4894..5247)
                     /gene="soxX"
                     /locus_tag="AC16QF_17020"
     CDS             complement(4894..5247)
                     /gene="soxX"
                     /locus_tag="AC16QF_17020"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HIR6450313.1"
                     /translation="MIARAALVLTTALLVVLAPDQAKAEAPDDLERGKAIASDRNAGN
                     CLSCHMMGDGELPGNSGPPLLQMRLRFPDRAVLRAQIWDATERNPDSVMPPYGRNLIL
                     TEEEIDRVVDYVLSL"
     gene            complement(5244..6182)
                     /locus_tag="AC16QF_17025"
     CDS             complement(5244..6182)
                     /locus_tag="AC16QF_17025"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6450314.1"
                     /translation="MYRFLSAALVVLAAGCGAPQEAAEPSKPTYDDSRVGHFGYGKPA
                     SAEEIAGWDIDIRPDGLGLPQGSGSVEDGEWLYEAQCAECHGSFGEGVGRFPVLSGGK
                     GSLTDPRPSKTVGSYWPYTSTLYDYIRRTMPFTQPESLTADETYAVTAYVLYLNDLVE
                     DDFVLTHENLASVRLPNEGNFVPDPRPDVSNTRCMTDCRDPAAISVLSEVPTYVPASM
                     TMEEIAEAEPAHPGAATYDQYCSICHDVGVAGAPRIGDAAAWSPRLERGMAALEMNAI
                     NGIASEAGVMPAKGGFAHLADDEVNAAVAYMVEGSQ"
     gene            complement(6166..7479)
                     /gene="soxC"
                     /locus_tag="AC16QF_17030"
     CDS             complement(6166..7479)
                     /gene="soxC"
                     /locus_tag="AC16QF_17030"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017134966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HIR6450315.1"
                     /translation="MRQRQLDQIVKSVAESGDKLPREERRRFLRQGLSLAGATAAASF
                     AGGALADSGNLPPNVPSWTRRLGPGVLTNPYGQPSPFESHVQRRTVDWLTADRIAAIS
                     FTPLGDLNGIITPSGVVFERYHAGAPAIDPNQHRLMIHGMVKRPIILTMDDLMRFPSV
                     SMIRFLECPANGGMEWRGAQMDRLQFSHGMLACCEWTGVLLSTLLEEVGLEADASWIL
                     AEGADGAHMSRSIPMEKALDDALIVYAQNGEALRPEQGYPVRLLNPGWEGNTSIKWLR
                     RLEVGDKPWHHREETSKYTDLMADGTAREFTFVQECNSVVTHPCPERPLKGKGKYFIE
                     GLAWSGRGKIRHVDVSVDGGVTWREARFTSQVLPRALTRFGLEWDWDGSPALIQSRAV
                     DETGYVQPTYGQLKAVRGTNSIYHKNAIHTWRLEPNGTVSNVQIS"
     gene            7616..7951
                     /locus_tag="AC16QF_17035"
     CDS             7616..7951
                     /locus_tag="AC16QF_17035"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6450316.1"
                     /translation="MELEVMQENAGRAADLMKLLGHPHRLMILCQLKEGECSVGELSE
                     KIGINQSPLSQHLARMRHEGVVSSRREAQTVYYSLAGPEVAAVISLLYELYCQPANEE
                     MQAPTSAAR"
     gene            complement(8009..8743)
                     /locus_tag="AC16QF_17040"
     CDS             complement(8009..8743)
                     /locus_tag="AC16QF_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450317.1"
                     /translation="MRALFLLSISLMSISCWQRDDLVPDMAILPELGNAPVQDPAHTE
                     AFEVQLNDVVYQVEPQYDYDLHGLVVSYRHHDGDRMLHRRWNDHLNVADLCVVWGANA
                     AQLDLNEFEFRNGQFTCFFKTRDQAAWRAFEKAGLSNNHLLTEDPYLRHMIERVRIGD
                     QIRIRGWLASYGNEYGFSRGTSTTRTDTGNGACETIYVRDFAILASMENGWRTVFDGS
                     ALLAVTLGLLWTVGVMGGRFEHRSEY"
     gene            8903..10855
                     /locus_tag="AC16QF_17045"
     CDS             8903..10855
                     /locus_tag="AC16QF_17045"
                     /inference="COORDINATES: protein motif:HMM:NF012927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR6450318.1"
                     /translation="MALYDNLFEPIEVGPVRIPNRIVRSPHGTGLLGEDLIAYHEARA
                     RGGVGMSTIQATGVHPGAPSGIPIFSDSCRPLLEQIAERMAPHGMKMFLQVYHPGAAG
                     GMGNAEAWSASAIPNPLAGVVPIAMTKTMIDDVVASFAAAAKRCCECGLDGVDVHASS
                     GYLIHEFLSPALNQRTDDYGGSLENRMRFMLEVIEAIRSELGDAAFAVGVRLPNHDYV
                     PGGLTPEMNAEIAQRVDPLVDYVSLHMGAYWRFHKLIAPSDDPLGVEMPSNEPITRVV
                     SKPTIVVGRIMTLDHASHIVDTGAADMVSMVRALIADPELVNKARRRDEHKIRPCIGS
                     NMGCVGQLMTAGRISCVVNIAAAREAKVTHDPETKAAAPKRIMVVGGGPAGLEFARTA
                     AMRGHEVELHEAMKVLGGQVTMAASAPHRADLGAITSWLAEEIDGLGVTVHLNSMVEP
                     DMIAERAPDEVVIATGTTPRADGFQVSTPAASVPGYDLPHVYSSWDLLGFGDITHIEG
                     PAVVFDDTGSFEAISVADVLLGAGVPVTMVSRLNAIGETLPYPPVTVGAARERLMSGD
                     FDFVGGHYLRSINPQEVEVGVLYTERSRRIAARTVILVSFNAPNREIAEALTAQGHTP
                     HLIGDVRARSSIMSAIHGAAELGRRI"
     gene            10909..>12774
                     /locus_tag="AC16QF_17050"
     CDS             10909..>12774
                     /locus_tag="AC16QF_17050"
                     /inference="COORDINATES: protein motif:HMM:NF025038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6450319.1"
                     /translation="MANELNPEQLLRRVDETGFPFATTADVVALEGPVAQTRAREAVD
                     FGVGIKFPGYNVYALGPAQTDKRALILARLKELAGGQDAPDDWCYLQNFEDPARPISL
                     RLPAGVGRRFRHHVQKFISSVRTMVPAALVSDEYRQKSQQLASSLQQRQSSDAAALEA
                     EARELELAMLPSPNGFIFAPVSDGKVMEEDAFMALDEGERQRIQNAIESMTDRLITRL
                     RDYPQFQQELVRQQRELVEKTAEQVITVLLARVRQRYQVYEEVMRYLARVQNELLANV
                     DKIVALERGPQTPMMPGVDPERFLERFNVNLIVDGSEADGVPVLYESNPSLDNLVGKL
                     EHRMELGTPVTDFNLIRPGALHRANGGYLLLDAERVIQKPFAWEALKRALADRSVRIE
                     SVSQLMSLTYSISLEPDPIPLDVKIVLLGSRRLYHLLRAYDPDFEELFKVVADFDDHV
                     DWSPENVDAYFGMIAQIVKQTGLKHLTAGAVARVLEHSGRMVEDRERLSTHIADVRDL
                     LSEADYIAGQTDATAIDREHVSKAVEQRIYRLDRFRELVRENVARGVILVESSGSRVG
                     QINGLSVVNLGQLSFGQPMRITATARLGRGELIDIERESKLGGNIHSKAVMIVSSF"
BASE COUNT         2114 a         4064 c         4225 g         2371 t
ORIGIN      
        1 gggcgcgctg aggaggatca gcaaatgaac acattgatca tcggtttcct catcggttgt
       61 gctttcggcg cgattcttta catgggcggt gccagcagct atcggcggat cctgggcact
      121 ctgctgctca aggacatgtg gatcatcaag ctcatggcta cggccattgg cgtcggcacg
      181 ctgggcgtct acctgctcga cctcggcggt ctcgcccaca tgtcgatcaa gcctgcctac
      241 atctggggga tcgccctggg aggcatcatc ttcgggatcg gttgggcggt ctccgggtat
      301 tgcccgggta cctgtgtggt gggcacgtca gagggcaaac gcgacgcgct gttcaccctg
      361 ctcggcgcct tcgtgggcgc tttcgtgttc tccctggtct ttccttacct ggagtccggg
      421 ctcatcgaac gagccaattt cggcagcatc accctcgagg acgtgctcgg tttcaacggg
      481 atttacctgg cgatcccgtt cagtcttgcg ctgatcgccc tggccttctt cgggttgcgg
      541 gaccgctacg ggcagtgacg gaagggcgcc gccacagcgg cgccgcccga cccttcaagg
      601 gtgcacgggc acgtagccct tagcgatctc ttcgatcatg aaagcgacgc cggtctgtac
      661 ctctctcacg ccgggcagaa cgaccggccg cttgccgtcg cgacgctcga tggtgtccag
      721 ggtgtttcca cagacgtaga aacgcacgcc gttctccatg aggttggcga tccgctgcgt
      781 gtgcggattg tccgtcgtca gaagtatctt gacgcctgag ctgaaggcga tgatctcgat
      841 gtccgccatg tcgggaccgc cgtagtgccg ggtgaggttg ttggctatgt ccagcaccgt
      901 ctggtggcgc gaaggatccg cgtcgctgac ctgaaggatg acgtgtgcct cggcgaatgg
      961 cttttcgtcg ttcatgcctt ctgccagggc gtcagcccat ggcaggctcg ccaggaggct
     1021 tgccagcgcg agcaggccgg cttgggggat agggttggat ctctgcatgg gtctcccagg
     1081 acggatcgat tctcaggagc aatcgtaccg cgcgatgccg ggatcgtctt tcacgccctc
     1141 gagcgtcggc tcgttggcct tgtcgatgcg gatggtgccc tggtcgcgca ggtaatcggc
     1201 gaccacgtcc cagacgggcg gccccggaga gcgctcgccg acggtggccc agccggccac
     1261 tttgtaggcg gccccggcct gcagcggagt gccgtcggtg agttcgaggt tcctgatccg
     1321 ttccccgttc ggcgcgtccg gcgcgcagct gtagcgcagc ccgccgaccc gcaccatgtc
     1381 gccaccctgc tggtagtagg gatccggatt gaagaggttg tctgccacgc tttccaagat
     1441 tacgcggatc tcttctccgc gcatttcccg gacgtaggtc tccggatagg tcatcccggt
     1501 ctggtccatc acgttctcca tcgtgatggt ctggcccggc agcacggacg ttccccaccg
     1561 aaaacccggg gaaaggctga tctgcgcatc gagaacctgg cgcagggcgt cgcagatcac
     1621 ctgatcgaac gtgccgttga agttgccgcg tcggtataaa agagaatcgg tgaccgcgag
     1681 gggctcctcc aggcgctgca gatacggctc gcgcacccga tcgatgtggg cctgcatctg
     1741 ggggtcgggc tcgagaaggt tcgcaaacac gggcagcagc cggtagcggt aaccgtcgat
     1801 gccggccgcg ccgaacttga tgtccagcac gccaacgaac tttccgttcg acccggcgtt
     1861 ggtcaccacc gttcttccac ccgggttggc gacgaccgcc ggtacgggga cgccatcgtg
     1921 agtgtggcca ccgagtatga agtcgattcc gggcacgcgg ccggccagct tcaggtccac
     1981 gtccatgccg ttgtgggaga gcaggaccac tgcatccacc cgctcccggg agcgaacctg
     2041 cgccacgagc tccgccagat ctgattcccg aataccgaag gtccagtccg ggatgaatcg
     2101 ctgggggttg gcgatggggg tataggggaa ggactggccg atgatggcga tccggcggcc
     2161 gtttagctcc ttgaccacgt acggcgggaa gacgtgtccg gtatcctcat cgaatacgtg
     2221 ggttccttcg aagagtgagt cctccgtggc ccggacgttc tgcgaaacga agtcggcgct
     2281 gagcagatct gcgttgtcga gcacttcctg ggcctggtag gtgaactccc agtgtccggt
     2341 catcacgtcg acgccgagaa ggttgcaggc ttccaccatg tcccgtccgc gggtccaaag
     2401 cgacgttccg gatccctgcc aggtgtcgcc gccatcgaga agcagcgccc cggactcgcc
     2461 gcgcagcgtg tcgatcagcg tcttcacgta ggcgaaacca ccgacccgac catagcgctc
     2521 ggcggcctcg gaaaagtcga tgcaggtgaa cgcgtgaacg tcgcgggggt tcgtcgaagt
     2581 aagcacgtcg agcaggttgc gacccaccag gtgaggcagc tggccgcgcg ctgcgcccac
     2641 gccgaggttg atgctgggtt cccggaagta gatgggattg agctgtgcgt gggtgtcggt
     2701 gatgtgcagc aggcgggcgt cgccccaggc cgggagcgcg tagtaatcgt cgggccgtgc
     2761 ggtgcgaagc gacgtgcagc cggaaagctg agcggtgatt gacgccgcgc cgaggatctc
     2821 caggaattct cggcggttca ttgggctggg ggcttgggaa agcggtcctg ccaggcgcct
     2881 tcttcggtgc gcgcctgcgc cagagatgcc tcggccagac ggagcgcttt ctcgccctgg
     2941 gcgcccgcgc tggcgaaatc gccccgggcg gccgccgtgc gcgcgtcttc gagggcgcgg
     3001 ctggcggtca cccacgcgtg ccccgcggcc tgagaatcag cgtaggccgc ctgggcggcc
     3061 tcgatgagcc acgttgctcg cgcggcggga tccagaccct caggtggctc ggctcgctgg
     3121 accgcagccg gtccgtctgt ggcgtcggct accgattcct gcccaggctc cgcgcagccg
     3181 gcacacagaa tgccgacgag ggagagaact ccgattttgc tcaggtagtt gatcatttgc
     3241 gcgttgccgg tccgttcagc tcgagcccgt tgctcatgta ggcgagaaag tactccaggt
     3301 tccggtaggg ctcgctctgc tcgtcgaacg gttccgcacc cacctggctg ttgcattcct
     3361 ggaagcgctt gtggaggctg ccgacgtcct cccatttgaa gcggtagacg ggccagtggg
     3421 tcacatggcc cagcgacgcg ctcaaccgct ccgcccgcag ccggttgccg gcaagctgta
     3481 cgtggcagct cgtgcacgcg aaattcagct ggcccctgcg cgtggcgtaa aagcgtttgc
     3541 cggcctggta ggcggctgcc gccgccgcgc tggcaggctc aggcacgttg atctgctgac
     3601 ctctcgaggc gtaggcgatg taggcggtga gcctggccat ctccgcccct tcgtaggcaa
     3661 gcggtgcctc cccgtgacta tcccggcagg cgtttacggc catgggcagc gtgaccacgg
     3721 tcgcttcctc ctcgctgaaa tgcgggaact gaccggccac gtccccctcg tcaccgaagc
     3781 agtcgctgta accggctccg ctggtgaagg gcgtggaaaa cagctcggca ccctcgtcga
     3841 cggcgaactc gtatggcggg aactcttcca tctccatcca ctgctcgcgc gcgccggcgt
     3901 cgatggcgta aacaccgtcc ctgtgatctt ccagcgcgat ctgcgggaag cggccggcgt
     3961 agtagtcgcg cagggccatg cgatcgctct ccggatccgc gcttgctcct gagcaggaaa
     4021 cgagcgcgag cacggcaatg gccgtgggcc gccgccaagc gacgggcctc ggtccgcgtc
     4081 ggcgttcagg cgcgctcact tgagaaccgc ttcgccggcc ccggtggctc cggtgttgtc
     4141 cacccaggtg atgcggatga cgtccccggg cgccgcgttc tttactctga aggagaggaa
     4201 gggattcttg gagatggaag ctccccagtc cgcagtcagc acttcctgat cgttcttgta
     4261 ggcggcgacc ttctcgatgt agtgggcggg gatcaggttg ccgtcggcgt cccggcgcgt
     4321 cccggtttcc atcgggtgca ccatgagaga ttttacggtc gctacgccgt ctgtgatctt
     4381 gccgcgaatt tttacgtcat ttgccatgtc gtcagtttcc tcgcttgtcg ctcagccgcc
     4441 gcagccgccg atggtgacct tcacctcttt gctcgtcgtg tagaccttgt ccgcggttcg
     4501 cacgtaagcg tgaacgacgg agctgtttcc catcttgacc cgggtggata cgtcggcgaa
     4561 ggcttccgga gatatgttga agctggccga caacgggttg gggttctcgt ccacgactat
     4621 gctgatgttc cgaacgccgg gcatgtccgt ggataccgtc acggggacga cggcgccgtt
     4681 ctcggcgatg tcggggattt tgaactcgat ggcggtactc tcttccaccg gcagatcgcc
     4741 gaagagctgc ttgagcgccg tgtttgcatc cgcggccttg aaagcggctg cggtatccgc
     4801 gagcaggcga agcggcagca gcactgcggc gccggccgtg gccatgctcg tgatccattt
     4861 gcgtcgattt aaagccatag cttcctcgtg gttttacagt gagagaacgt agtcgaccac
     4921 ccggtcgatc tcctcctcgg tcagaatcag gttgcgtccg tagggcggca tgaccgaatc
     4981 cggattgcgc tcggtggcgt cccagatctg cgctcgcagc acggcccggt ccgggaatct
     5041 caggcgcatc tgcagcaggg gcgggccgct gttgcccggc agctcgccgt ctcccatcat
     5101 gtgacaggac aggcagttgc cggcattgcg atcagatgcg atggccttgc cccgctccag
     5161 gtcgtcgggg gcctcggcct tggcctggtc cggcgccaaa acgaccagca gggcggttgt
     5221 caggaccagc gcggctcgtg caatcattga gaaccctcca ccatataggc caccgctgca
     5281 ttgacctcgt cgtccgccag gtgcgcgaag ccgccttttg ccggcatgac gccagcctcc
     5341 gatgcgattc cgttgatggc gttcatttcc agggccgcca tgccgcgctc gaggcgcggt
     5401 gaccaggcag cggcgtcgcc gatgcggggt gcgccggcga caccgacatc gtgacatatg
     5461 ctgcagtact gatcgtacgt cgccgcgccc ggatgggccg gttcggcctc ggcgatctcc
     5521 tccatggtca tggaggccgg cacgtaggtt ggaacttcgg agagcacgct gatggccgcg
     5581 gggtcgcggc aatcggtcat gcagcgcgtg ttgctcacgt ccggccgggg gtccggcacg
     5641 aagttgccct cgttgggaag acgcacggag gccagatttt cgtgggtgag cacgaagtcg
     5701 tcttcgacca gatcattcag gtacaggacg taggcggtca ccgcgtacgt ctcgtcggcc
     5761 gtcagggact ccggctgcgt gaagggcatg gtgcggcgga tgtagtcgta cagggtgctg
     5821 gtgtagggcc agtagctgcc aacggttttg ctgggccgcg ggtctgtcag cgagcccttt
     5881 ccgccagaca gcacggggaa gcgcccgacc ccttcgccga aggatccgtg gcactcggcg
     5941 cactgcgcct cgtagagcca ctcgccgtcc tcgacggatc cgctcccctg gggaagtccc
     6001 aggccgtccg gtctgatgtc gatgtcccac cccgcgattt cctcggcgct cgccggcttg
     6061 ccgtagccga agtggccgac gcggctgtcg tcgtacgtcg gcttggatgg ttcggccgcc
     6121 tcctgggggg caccgcagcc agcggcgagg acgacgagcg cggcactaag aaatctgtac
     6181 attgctcacc gtcccgttgg gttccagccg ccaggtgtga atggcgttct tgtgatagat
     6241 ggagttggtg ccgcgcacgg ccttgagctg cccgtaggtg ggctgaacat agccggtctc
     6301 gtcgacggcg cggctctgga tcagtgcggg cgagccgtcc cagtcccact ccaggccgaa
     6361 tcgcgtcagc gcccttggca gcacctggct ggtgaagcgc gcttcccgcc aggtcacgcc
     6421 tccatccacc gagacgtcca cgtgcctgat cttgccgcgg ccggaccagg cgagcccttc
     6481 gatgaaatac tttcctttgc ccttgagggg cctttcgggg cagggatggg tcacgacgga
     6541 gttgcactcc tggacgaagg tgaactcgcg tgcggtgccg tctgccatca ggtcggtgta
     6601 cttggaggtc tcttcgcggt gatgccacgg tttgtcgccg acctcgagcc gtcgaagcca
     6661 cttgatcgag gtgttgccct cccatcccgg attcagcagg cgcaccggat atccctgctc
     6721 cggccgcagg gcctcgccgt tctgggcgta cacgatgagc gcatcgtcca gcgccttctc
     6781 catggggatg ctgcggctca tgtgggcgcc gtccgcgcct tcggccagga tccagctggc
     6841 gtcggcctcc agcccgacct cctcgagcag ggtggagagc agcacgcccg tccactcgca
     6901 gcaggcgagc atgccgtggc tgaactgcag ccggtccatc tgggcgcctc gccactccat
     6961 gcctccgttg gcgggacatt cgagaaaccg gatcatgctc accgacggga agcgcatcag
     7021 atcatccatg gtcagaatga tcgggcgctt gaccatgccg tggatcatca accggtgctg
     7081 gttcgggtcg atggccggcg cgccggcgtg atagcgctcg aacaccacgc cgctcggcgt
     7141 gatgatgccg ttgagatcgc ccagaggcgt gaagctgatg gcggcgattc ggtccgccgt
     7201 cagccagtcc acggtgcgcc gctggacgtg ggattcgaag ggggagggtt ggccgtaggg
     7261 gttggtcaga acgcccggac ccagccgccg ggtccatgac gggacgtttg gaggaaggtt
     7321 gcccgaatcc gcgagcgccc cgccggcgaa gctcgcagcc gccgtggctc cggcgaggct
     7381 caaaccctgc cgcaggaagc gtcttcgctc ctcccgtggc agcttgtcgc cggactcggc
     7441 aacggacttc acgatctgat ccagctgacg ttgacgcacg ctccctccgc gtatattttc
     7501 cgtcgcgcga tgcgaagagt tagtatatta taaatttctt aagtaaaggc aagccgaaat
     7561 atcggctgga tttggagggt cgaaaaacgg cggcccgcca tcggagtatt cgttgatgga
     7621 actcgaagtc atgcaggaaa acgccggccg ggcggccgac ctgatgaagc tgctcgggca
     7681 cccgcaccgg ctcatgattc tctgccagct caaggaaggc gaatgctccg tgggcgagct
     7741 cagcgagaag atcggcatca atcagtcgcc gctgtcccag catcttgccc gcatgcgcca
     7801 cgagggcgtg gtgagcagca ggcgcgaggc gcagacggtc tactactcgc tcgccggccc
     7861 ggaagtcgca gcggtgatct cgctgctcta cgagctctac tgccaaccgg cgaacgagga
     7921 gatgcaggcg ccgacttcgg cggcccgctg agccccctcg agacgctttc aggcgtcagc
     7981 cgtccttatc acagtcggtt ctacccggtc agtattcgga tcggtgctcg aagcgcccgc
     8041 ccatgacgcc caccgtccac agcagcccca gggtgacggc cagcagagca ctgccatcga
     8101 aaacggtgcg ccagccgttt tccatcgacg ccagaatcgc gaaatcccga acgtagatgg
     8161 tctcgcacgc gccgttgccg gtgtccgtac gcgtcgtgct cgttccccgt gagaaaccgt
     8221 actcgttgcc gtagctcgca agccatcccc ggatgcggat ctggtcgccg atccttaccc
     8281 gttcgatcat gtgccgaagg taggggtcct cggttagcag gtggttgttg gacagcccag
     8341 ccttttcaaa cgcgcgccag gcggcctgat ccctggtttt gaaaaagcac gtgaactggc
     8401 cgttcctgaa ctcgaactcg ttcaggtcga gctgcgcggc gttcgcaccc caaaccacgc
     8461 acagatcggc gacgttgaga tgatcgttcc agcgacggtg gagcatgcgg tcgccgtcgt
     8521 gatgccgata ggaaaccacc agcccgtgga ggtcgtagtc gtactggggc tccacctggt
     8581 agacgacgtc gttcagctgg acttcgaacg cctccgtgtg cgccgggtcc tgaaccggcg
     8641 cattgccgag ttctggaagg atcgccatgt ccgggacgag gtcgtctcgc tgccagcagg
     8701 aaatggacat cagactgatc gatagcagga acagtgcccg caccaaccgc tccactgaga
     8761 aatcctcatt gggccgattc gtgcattgtc cgtaacgggt gcgacggagt cgtggacgag
     8821 aggcacgttt ttgcccgttc ctgcggttcc tgtttgagcc aagccgggct agagtccagg
     8881 gttgcagagg gaggaatacg ccatggcgct ttacgacaac ctgttcgagc ccatcgaggt
     8941 cggccccgtg aggataccga accggatcgt ccgctcgccc cacggaaccg ggctgctcgg
     9001 agaagatctg atcgcctatc acgaggcgcg cgcgcgcggc ggcgtcggga tgtcgaccat
     9061 ccaggcaacg ggcgttcacc ccggggcacc ttcgggcata ccgatcttct cggacagttg
     9121 ccgcccgctg ctcgagcaga tcgccgagcg catggcccct cacgggatga agatgtttct
     9181 gcaggtctac catcccggcg cggccggcgg catgggcaac gcggaggcct ggtccgccag
     9241 cgccattccc aaccccctcg ccggcgtcgt gcccatcgcc atgacgaaga ccatgatcga
     9301 cgacgtggtg gccagcttcg ccgccgcagc gaagcgctgc tgcgagtgcg ggctggacgg
     9361 cgtcgacgtg cacgcctcga gcggttacct gattcacgag ttcctctcgc cggcgctcaa
     9421 ccagcgcacg gacgactacg gcgggtctct ggaaaaccgc atgcgtttca tgctcgaggt
     9481 catcgaagcc atccgcagcg agcttgggga cgcggccttc gccgtgggcg tgcgcctgcc
     9541 gaatcacgac tacgtccccg gcggcctgac gccggagatg aacgcggaga ttgcccagcg
     9601 cgttgatccg cttgtcgact acgtctcgct ccacatgggg gcctactggc ggttccacaa
     9661 gctcatcgcg ccctccgacg accccctcgg ggtggagatg ccctctaacg agccgatcac
     9721 gagggtggtt tcgaagccga ccatcgtggt cgggcgcatc atgaccctcg accatgccag
     9781 ccacatcgtc gacacgggcg cggccgacat ggtttccatg gtccgggcgc tgatcgccga
     9841 tccggagctg gttaacaagg cgcgccgccg ggacgagcac aagatccggc cgtgcatcgg
     9901 gtcgaacatg ggatgcgtcg gccagctcat gaccgccgga cgcatcagct gcgtggtgaa
     9961 catagccgcg gcccgcgagg ccaaagtgac ccacgatccc gagaccaagg ccgcggcacc
    10021 caaacgcatc atggtggtcg gcggcgggcc ggccggcctc gagttcgcgc gcacggctgc
    10081 catgcgcggt cacgaggtgg agcttcacga agccatgaag gtgctcggcg ggcaggtgac
    10141 catggcggct tcagcgcctc accgggcgga cctcggcgcc atcacgagct ggctggcgga
    10201 ggagatcgac gggctcggcg tgaccgtgca tctcaactcc atggtggagc ctgacatgat
    10261 tgccgagcgg gctccggacg aggtggtgat cgcaaccgga acgacgcccc gcgcggacgg
    10321 gtttcaggtg tccactcccg cagcctcggt gcccggctac gacctgcccc acgtctactc
    10381 ctcctgggac ctgctcggct ttggcgacat cacccacatc gaggggccgg cggtggtgtt
    10441 cgacgacacc ggctcgtttg aggcgatctc ggtggccgac gtgctgctcg gcgccggcgt
    10501 gccggtgacc atggtttcgc gcctgaacgc catcggcgag accctccctt acccgcccgt
    10561 taccgttggc gcggcccggg agagattgat gtccggtgat ttcgatttcg tcggcgggca
    10621 ctatctgcgc tcgatcaacc cccaggaggt ggaagtgggg gtgctttaca ccgagcgcag
    10681 ccgacgcatc gcggcgcgca cggtgatcct ggtgtctttc aacgcaccca acagagaaat
    10741 cgccgaggcg ctcacggccc agggccacac gcctcacctc attggcgacg tgcgggcgcg
    10801 cagcagcatc atgtccgcca ttcatggggc ggccgagctg gggcggcgca tctgatactg
    10861 cggcaatcca cgatagcggg tgccgggagg ggaggctaga aaggcggcat ggcaaacgaa
    10921 ctcaatcccg agcagctgct tcgccgtgtg gacgaaaccg gattcccctt tgcgaccacg
    10981 gcggacgtcg tggcgttgga gggcccggtg gctcagaccc gggcccggga ggccgtcgac
    11041 ttcggcgtcg ggatcaagtt tcccggctac aacgtgtacg ccctcgggcc ggcgcagacc
    11101 gacaagaggg cgctgatcct tgcgcggttg aaggagcttg ccggcggcca ggacgctccc
    11161 gacgactggt gctacctgca aaacttcgaa gaccccgcgc gaccgatcag cctgcgtctg
    11221 ccggctggtg tggggcgccg tttccggcac cacgtccaga agttcatcag cagcgtgcgg
    11281 accatggtgc cggctgcgct ggtgtccgac gagtaccgtc agaagagcca gcagctggca
    11341 agctctctgc agcagcgtca gagcagcgac gccgctgccc tggaagccga ggcgcgggag
    11401 ctggaactcg ccatgctgcc ctcgccgaac ggcttcatct ttgcccccgt gagtgacggc
    11461 aaggtgatgg aagaggatgc cttcatggcg ctcgacgaag gcgagcgtca gcgcatccag
    11521 aatgccatcg aatcaatgac ggaccgactg atcacgcgcc ttcgcgatta tccccagttt
    11581 cagcaggagc tcgtccggca gcagcgtgag ctcgtggaga agacggccga gcaggtcatc
    11641 acggtgctgc tcgccagagt ccgccagcgc taccaggttt acgaagaggt gatgcgatat
    11701 ctcgctcggg tgcagaatga gctgcttgcg aacgtggaca agatcgtcgc tctggagcga
    11761 gggccgcaga cgccgatgat gccgggagtc gatccggagc gcttcctgga gaggttcaac
    11821 gtcaacctga tcgtcgacgg ctccgaggcg gacggcgtgc cggtgctcta cgagagcaat
    11881 ccgagcctcg acaacctcgt gggcaagctc gagcaccgga tggagctcgg gacgccggtg
    11941 acggatttca acctgatccg cccgggcgcg ctccaccggg ccaacggggg ctacctgctg
    12001 ctggatgccg aacgcgtgat ccagaagccg ttcgcctggg aagcgctcaa gcgagcgctg
    12061 gccgaccgct ctgtacggat cgaatcggtg agccagctga tgagcctgac ctattccatc
    12121 tcgttggagc cggatccgat ccctctggac gtgaagatcg ttctgctcgg atcgcgccgc
    12181 ctctaccacc tgctgcgcgc ttacgatccg gacttcgaag agctgttcaa ggtggtggcc
    12241 gatttcgacg atcacgtgga ctggtcgccc gagaacgtgg acgcctactt cggcatgatc
    12301 gctcagatcg tcaagcagac gggtctcaag cacctcacgg ccggtgccgt ggcgcgggtc
    12361 ctcgagcaca gcgggcggat ggtcgaagac cgtgagcgtc tctccacgca catcgccgac
    12421 gtgcgcgatc ttctcagcga agcggactac atcgctggtc agacggacgc gaccgccatc
    12481 gaccgggagc atgtctccaa agcggtggag cagcgcatct accgcctcga ccggtttcgc
    12541 gagctggtgc gggagaacgt ggcgcgcggg gtgatcctgg tcgagtcgtc cggttcccgc
    12601 gtcggccaga tcaacggcct gtcggtggtc aatctcggtc agctgagctt cggccagccc
    12661 atgcggatca cggcgaccgc ccggcttggc cggggcgagc tgatcgacat cgagcgtgaa
    12721 tccaagcttg ggggcaacat ccattccaag gcggttatga tcgtctcgtc cttt
//
LOCUS       DBNHLM010000366         2669 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1134565, whole genome shotgun sequence.
ACCESSION   DBNHLM010000366 DBNHLM010000000
VERSION     DBNHLM010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1134565"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="AC16QF_17055"
     CDS             complement(<1..694)
                     /locus_tag="AC16QF_17055"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6450320.1"
                     /translation="MLKTENYRTMLVERRENGVGVLTLNRPERLNAVNGTMHSELARF
                     SLDFASDPELRVLVITGAGRAFCAGGDFSPDDPVGSNPDGTDMWAEARQIVDRMLECE
                     KPIISAVNGYAMGLGATVALLADVVVVSPKAVIADTHVAMGIGAGDGGQLIWPHLIGV
                     NRAKYYLMSGDRIDGREAAEMGLASFCVEDHDDLMPKALEIAHRLAAGAPQAIAASKA
                     AVNAYLRSVSAMV"
     gene            838..>2669
                     /locus_tag="AC16QF_17060"
     CDS             838..>2669
                     /locus_tag="AC16QF_17060"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR6450321.1"
                     /translation="MTTIAGADASFEICWDHLGIPHVYASTVADAYRGMGYVEGYERL
                     WQIHLSCLYANGNAAADMGERFVAQDALHRAFGVPASYDLPESPGDWVVDAYLEGLNA
                     YVDTLDDPPPEFRHTGALPRRFTRADVAARYRFTNFFQHRSWPNKLMLGRLMATHGVE
                     WFRGHLLRFSDEDEKLIELLREPLKSLNLRPINLLFPDAPVSGSNNWVVTGSLSASGK
                     PMLGMDPHQAYSIPNTFFYVHLHAPGWDVFGASFPGVPYFMMGYTKKIAWGLTTGFVD
                     NYDVYVEEIAGDRYRTPDGWAEVERRTETIGVRGGTTREVEIASTRHGPLLEPLTDAL
                     EMTANGPRRYQTAVSWNLTHLETSAGALALLPLAEDAAGFGDALFEGDVCPLVNNIIC
                     VDTKDDIRRFVAATLPARKGVTGMVPLPGWDPDCDFRWSRADELLVEQAPASGFALTA
                     NNDTMGDLGPFPIHNFPTLPARAQRIRDLLEAGGSADGKFTPADFERMQLDLYDYRAR
                     DQIPGILMALEGREDDDLELAAALLRRWDFRASGESAAACLYYPFVDRVWQRTFMMAV
                     LDDDVLRGIPLAAPGLTRFDVDAFLAEGSPWRAHRELLFLTMAEV"
BASE COUNT          453 a          915 c          830 g          471 t
ORIGIN      
        1 tgaccatcgc cgagacggag cgaagatagg cgttcaccgc cgccttcgac gcggcgatcg
       61 cctgaggcgc gccagcggca agccggtgcg ctatctccag cgccttcggc atgaggtcgt
      121 cgtgatcctc cacgcagaag ctggcgagcc ccatttctgc ggcctccctc ccgtcgatcc
      181 gatcccccga catcaggtag tacttggcgc gattcacacc gatcaggtgc ggccagatga
      241 gctggccccc gtcacccgcg ccaatgccca tggccacgtg ggtgtccgca atcacggctt
      301 tgggagacac gaccacgacg tcggccagca gcgcaacggt agcgccaagc cccatggcgt
      361 acccgttgac ggccgagatg atgggttttt cgcattccag catgcggtcg acgatctgcc
      421 gggcttccgc ccacatgtcg gtcccatcgg gatttgaacc caccgggtcg tcggggctga
      481 aatcgccgcc cgcgcagaac gcccggcccg cgccggtgat gaccagcacg cgcagttccg
      541 ggtcgctggc aaagtccagg ctgaagcgcg caagctccga atgcatggtg ccgttcaccg
      601 cgttgagacg ctcggggcga ttgagcgtaa gcacaccgac gccgttctcc cgtctctcga
      661 cgagcatggt ccgatagttt tccgtcttga gcatgtgaga tgtcctccga gttgtcagcc
      721 agctttgttg aaacctgcac caccatcttc gatccgctac tctacggcga aacggtccgg
      781 cagagaattc atcatccccc ggacacccac ccaagtgctc gatagaggaa taccagcatg
      841 acgaccatcg caggagcgga cgcctcgttc gagatctgct gggaccacct gggaatcccc
      901 cacgtgtatg cctccacggt cgccgacgcc taccggggca tgggctacgt ggagggttac
      961 gagcgcctgt ggcagattca cctgagctgc ctgtacgcca acggcaatgc cgccgccgac
     1021 atgggagagc gattcgtggc tcaggacgcc ctgcatcgcg ccttcggcgt gcctgccagc
     1081 tacgatcttc ccgagagtcc cggtgactgg gtggtggacg cctatctcga gggattgaac
     1141 gcctacgtcg acaccctcga tgaccctccc cccgagttcc gccatacggg cgccctgccc
     1201 cggcgcttca cgcgggccga cgttgccgcg cgctaccgat tcaccaactt ctttcagcac
     1261 cggtcgtggc cgaacaagct catgctcggc cggctgatgg ccacccacgg cgtggagtgg
     1321 ttccgcggcc acctgctgcg cttctccgac gaggacgaga aactcatcga gctgctgcgt
     1381 gagcctctca aatcgctcaa cctcaggccc atcaacctgc tcttcccgga tgcgccggta
     1441 tccggcagca acaactgggt ggtgactggc agcctgtccg cttccggaaa gcccatgctg
     1501 gggatggatc cacatcaggc ctactcgatc ccgaacacgt tcttctacgt gcacctccac
     1561 gcgcccggct gggacgtgtt cggcgcttcg tttcccggcg tgccctactt catgatgggc
     1621 tacacgaaga agatcgcctg gggactcacc accgggtttg tggacaacta cgacgtctac
     1681 gtcgaggaga tcgccgggga ccgctaccgc acgcccgatg gctgggccga ggtggaaagg
     1741 cggaccgaga ccatcggggt ccggggcggc accacgcggg aggtcgagat tgcctcgacg
     1801 cgtcacggac cgctgctcga gcccctgacc gacgcgctcg aaatgaccgc caacgggccg
     1861 cggcgctacc agaccgcggt gagctggaac ctcactcatc ttgagacgtc ggccggcgcg
     1921 cttgccctgc tgccgctcgc cgaggatgcc gccgggttcg gcgacgcgct tttcgaaggt
     1981 gacgtctgcc cgcttgtcaa caacatcatc tgcgtcgaca ccaaggacga catcaggcgc
     2041 ttcgtcgcgg cgaccctgcc cgcacgcaag ggcgtgacgg gcatggtccc gcttcccgga
     2101 tgggatcccg actgcgactt tcggtggtcg cgagccgatg agcttctggt cgagcaggcg
     2161 cccgcctcgg gcttcgcgct tacggccaac aacgacacca tgggcgattt gggacctttc
     2221 ccgatccata acttcccgac gctgccggcg cgcgcgcagc ggattcgcga cctgctcgaa
     2281 gcgggcggct ccgcggacgg gaagttcacc ccggccgatt tcgagcgcat gcagctggac
     2341 ctttacgact accgggcaag ggatcagatt cccgggatcc tcatggcgct ggaaggtcgc
     2401 gaggacgacg acctcgagct cgccgcagcc ctactgcgcc gatgggattt ccgggcgagc
     2461 ggcgagtcgg cggccgcatg cctttactac ccgttcgtgg atcgggtctg gcagcggacg
     2521 ttcatgatgg ccgtgctgga cgacgacgtc cttcggggca ttccgctggc ggcgccgggc
     2581 ctcacccgct tcgacgtgga cgcgtttctc gccgagggat caccatggcg cgcgcaccgg
     2641 gaactgctgt tcctgaccat ggccgaggt
//
LOCUS       DBNHLM010000367         4123 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_57063, whole genome shotgun sequence.
ACCESSION   DBNHLM010000367 DBNHLM010000000
VERSION     DBNHLM010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_57063"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /gene="bamA"
                     /locus_tag="AC16QF_17065"
     CDS             complement(<1..1495)
                     /gene="bamA"
                     /locus_tag="AC16QF_17065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HIR6450322.1"
                     /translation="MTGRGARIVVLLALLGSASLARADAFTISDIRLQGLQRVSAGTV
                     FNVLPVSVGDTIDEVSVRQLVRLLFRSGYFDDIRMARDGDILVVTFKERPAIESIEID
                     GNKAIKTDALLDGLGQQGLREGEIFKQATLERVGLELERQYVSQGRYGAAIDTQVEQL
                     PRNRVAIKIEIEEGKTSGIRHINIVGDEVFEQEELLDQLELQHPSLFSFYRNDDKYAR
                     EKLTADLEKLESYYRDRGYVEFDIESTQVSITPDRRQVYITVNIEEGDKYTVSGVNLI
                     GELSDVNPDDLKRLFLVAEGQVFSQALVTATEERLTQALGNSGYTFASASGVPRVSED
                     GTVEVVFNVDSGKRAYVRRVSFAGNKVTQDEVMRREMRQMEGGWASTAQIDFSKVRLE
                     RLGYFKGVSVETPEVPGTDDQIDVEFTVEEQPSGSVSATLGYSQGYGLIVGGNYQENN
                     VLGTGNSLGLGISYSDFQQSISFNYFNPYYTLDSISRGFNLFARRLDY"
     gene            complement(1500..2834)
                     /gene="rseP"
                     /locus_tag="AC16QF_17070"
     CDS             complement(1500..2834)
                     /gene="rseP"
                     /locus_tag="AC16QF_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017479411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR6450323.1"
                     /translation="MDFLQYPLALLVTLGVLVTFHELGHFVIARWSGVTVVRFSVGFG
                     KPFWSWRDRRGTEFALAYIPLGGYVRMLDEREPGVIEYDRRSTDVSYNELSVWWRMAI
                     ALGGPAANFLLAIIVYWVLFVIGTITYAPLLGSVGADSAIGRAGLREHEELIAVDGVE
                     TSSWQAVNMALAGRLGESGEIELTTRRPGSDSVRSGAVPISSWHTGVDEPDLLGSLGI
                     VPALPPIVAQVVPDSPAERAGFRRWDRIVSVDGEAVADWGQWVDLIRNAPDRRLSVTV
                     DRSGQVLVLDVTPDVQEAAGGEVTGFLGVGAYTKEIRYGVLAAVPKSLWESWDKTALT
                     LGLLKKMITGHVSVKNLSGPITIAKVAGDSARSGWRFFFSVLALLSISLGVLNLLPIP
                     ILDGGHILFCGAEAVTGRPVSERVQILGTQIGLFMVASLMLLAIYNDISRLL"
     gene            complement(2955..3746)
                     /locus_tag="AC16QF_17075"
     CDS             complement(2955..3746)
                     /locus_tag="AC16QF_17075"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007949337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR6450324.1"
                     /translation="MLRTRILTALIIAPLAVAALYLLPASWFAVAYGLVAALGAYEWG
                     GLIGLRRFWSRLAYAALFVVAAGMTWWLDRGFGLAIVVGSIVWIVAAGLVLTYPATAA
                     VVREPWSNGLLGLFVLWGAWSAVVEVRGEPGSGGANWVMWLLVLVWSADVGAYFAGRR
                     FGRRKLAPAVSPGKTWEGAFGGMLVSLALCGGALLVLGLPAWWLLAVAGLVVVSIFGD
                     LFESVIKRVRGVKDSSNLLPGHGGILDRIDSLLAVLPVFALILAR"
     gene            complement(3827..>4123)
                     /locus_tag="AC16QF_17080"
     CDS             complement(3827..>4123)
                     /locus_tag="AC16QF_17080"
                     /inference="COORDINATES: protein motif:HMM:NF013424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HIR6450325.1"
                     /translation="DIAQAARALARQVREGECSLEEIDEERFAAHLSLGGLPDPDICI
                     RTGGDHRISNFLLWDFAYTELYFTDTYWPDFDAACLHEALNWFDDRQRRFGRRT"
BASE COUNT          807 a         1390 c         1264 g          662 t
ORIGIN      
        1 cgtaatcgag ccggcgggca aagaggttga atccccggct gatactgtcc agcgtgtagt
       61 aggggttgaa gtagttgaag ctgatggact gctggaagtc cgagtagctg atgccgagcc
      121 cgaggctgtt cccggtgccg agcacgttgt tctcctgata gttgccgccg acgatgagac
      181 cgtaaccctg ggagtagccg agcgtcgcgg agacgctccc cgacggctgc tcttccaccg
      241 tgaactccac gtcgatctga tcgtcggtcc ccggaacctc gggcgtttcc acgctgacgc
      301 ccttgaaata gccgagccgc tcgagccgga ccttggagaa gtcgatctgc gccgtggaag
      361 cccagccccc ttccatctgc cgcatctctc tgcgcatcac ctcgtcctgg gtgaccttgt
      421 tgccggcaaa ggaaacacgg cgcacgtatg cccgcttgcc ggaatccacg ttgaacacga
      481 cctcgaccgt gccgtcctcg ctgacgcgag gtacgcccga ggcggatgcg aacgtgtaac
      541 cggagttgcc gagggcttgg gtgagccgtt cctccgttgc ggtgacgaga gcctgagaga
      601 aaacctgccc ctcggccacg aggaacagac gcttgaggtc gtcggggttc acgtcgctga
      661 gctcgccgat caggttgacg ccgctgaccg tgtacttgtc gccttcctct atgttcaccg
      721 tgatgtagac ctggcgccgg tccggcgtga tgctcacctg ggtcgactcg atgtcgaact
      781 ccacatagcc gcgatcgcgg tagtaggact ccagcttctc cagatcggcc gtgagcttct
      841 cccgcgcgta cttgtcgtcg ttgcggtaga aggaaaagag gctcgggtgc tgcaactcca
      901 gctgatcgag cagctcttcc tgctcgaaga cctcgtcgcc gacgatgttg atgtgccgga
      961 tgccggaagt ctttccttcc tcgatttcga tcttgatggc cacacgattc cggggcagct
     1021 gctcaacctg ggtgtcgatg gcggcgccgt aacggccctg agaaacgtac tgccgctcca
     1081 gctccagccc gacccgctcc agggtcgcct gcttgaagat ttccccctcc cgcagtccct
     1141 gctggcccag cccgtcgagg agcgcatccg tcttgatcgc cttgttgccg tcgatctcga
     1201 tgctctcgat ggccggccgt tccttgaacg tgaccaccag gatgtcgccg tcgcgagcca
     1261 tgcggatgtc gtcgaaatag cccgacctga acagcaggcg taccagctgg cggacgctca
     1321 cctcgtcgat ggtgtccccg acgctcacgg ggagcacgtt gaaaaccgtc cccgcggaca
     1381 cccgctgaag accctgcaaa cgtatgtccg atatggtgaa ggcgtctgcc cgagccagac
     1441 ttgccgaccc gagcagggcc agcagcacaa cgattcgcgc tcccctcccc gtcatcgttt
     1501 cagagcagcc tggagatatc gttgtagatc gccagcagca tcaggctggc caccatgaac
     1561 aggccgatct gggtccccag aatctgaacc cgctcggaga ccggccggcc ggtaaccgcc
     1621 tccgccccgc aaaacaggat gtggcccccg tcgagaatgg gaatgggcag caggttgagc
     1681 acgcccagcg agatgctgag cagcgcgaga acgctgaaga agaagcgcca ccccgagcgg
     1741 gccgagtcac cggccacctt cgcgatggtg atgggcccac tgagattctt gacggacacg
     1801 tgcccggtga tcatcttctt gagcaggcca agcgtcagcg cggtcttgtc ccaggactcc
     1861 cagaggctct tgggaacggc agcaagcacg ccgtaccgga tctccttggt gtacgcgccc
     1921 accccgagga agccggtcac ctcgccgccg gcagcttcct ggacgtccgg cgtcacgtcc
     1981 agcaccagca cctgtccgga acggtctacg gtcacgctca agcgccggtc cggggcattg
     2041 cgtatcagat ccacccattg gccccagtcg gcgaccgcct ctccatcaac agagacgatc
     2101 cgatcccagc gacggaaccc ggcgcgctct gccgggctgt ccggcaccac ctgggcgacg
     2161 atggggggca gtgcgggcac gatcccgagc gagccgagca gatccggttc gtcaacaccc
     2221 gtgtgccagc tggaaatcgg cacggcaccg gagcgtaccg aatccgatcc gggtctccgg
     2281 gtagtgagct cgatctcgcc gctctcgccc agccgccccg cgagcgccat gttcaccgcc
     2341 tgccaggagg acgtctcgac gccgtccacg gcgatgagct cctcgtgctc gcgcagcccc
     2401 gccctgccga tggcggaatc cgcccctacg ctgcccaaaa gcggggcgta ggtgatcgta
     2461 ccgatcacaa agagcaccca gtagacgatg atggccagca ggaaattggc agccggcccg
     2521 cccaaagcga tggccatgcg ccaccagacg ctgagctcgt tgtaggagac gtccgtgctg
     2581 cggcggtcgt actcgatgac gccaggctcc cgctcgtcga gcattctcac gtagccgccc
     2641 agcggaatgt acgcaagagc gaactcggtg ccgcgccggt cgcgccacga ccagaaaggt
     2701 ttcccgaagc cgaccgagaa gcggacgacg gtgacgcccg accagcgcgc gatgacgaaa
     2761 tgacccagtt cgtggaaagt caccaggacg ccgagagtca ccagcagggc aaggggatat
     2821 tgcagaaaat ccataaactg agtgcgacag atgccccgta tcaaaacttc agacagcagc
     2881 ggtgctcgcc ggttcgcccc gccctggaat tacccgaccg tcgtaaagac catcggcgag
     2941 cgcatcataa tggatcagcg ggcgagaatc agcgcgaaaa ccggcagcac cgccaggagg
     3001 gagtcgattc tgtccaggat acctccgtgt ccgggaagta agttgctcga atctttgacg
     3061 ccccgcaccc gcttgatcac gctctcgaaa aggtcaccga atatggagac cacgacgagc
     3121 cctgcgaccg cgagcagcca ccaggctggc agacccagca ccagcaaagc gccaccgcaa
     3181 agcgcgaggc tgaccagcat gccgccaaag gccccttccc aggtcttgcc ggggcttacg
     3241 gccggcgcca gcttgcgccg gccgaatcgc cgccctgcga aataggcccc cacgtcggcg
     3301 ctccagacca gcacgagcaa ccacatgacc cagttggctc cgccgcttcc gggctcgcca
     3361 cgcacctcta cgaccgccga ccatgcgccc cagagaacga acaggcccaa caggccgttg
     3421 ctccagggtt cccggacaac ggcagcggtc gcggggtatg tcaataccag gcccgccgcc
     3481 acgatccaga cgatggatcc gaccacaatg gccagcccga accctctatc gagccaccag
     3541 gtcatcccgg cggccaccac gaacagcgcg gcgtaagcca gacgcgacca gaaccggcgc
     3601 agcccgatga gcccccccca ctcgtaggcg cccagggcag ccaccaggcc gtaggcaacg
     3661 gcgaaccagg aggcggggag cagatacagc gcagcgacgg cgagcggcgc tatgatcagg
     3721 gcagtgagga tccgggtccg cagcacggct cagaccactg tcggtcgcat cggtgaggac
     3781 ggctgccggg gtagagctcg ccgctcgtgc ccgggcaacg ggctcgtcat gttcgacggc
     3841 cgaagcgacg ctggcggtca tcgaaccagt tcagcgcttc atgcaggcag gccgcatcga
     3901 aatccggcca gtacgtgtcg gtgaagtaga gttcggtgta ggcgaaatcc cagagcagga
     3961 aattgctgat ccgatgatct ccgcccgtcc ggatacagat gtcgggatcg ggtagcccgc
     4021 ccagggagag atgggcggca aaccgttcct cgtcgatttc ctccaggctg cattcgccct
     4081 cgcgaacttg ccgcgcaagg gcgcgggcgg cctgggcaat atc
//
LOCUS       DBNHLM010000368         4168 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_117200, whole genome shotgun sequence.
ACCESSION   DBNHLM010000368 DBNHLM010000000
VERSION     DBNHLM010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_117200"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="AC16QF_17085"
     CDS             <1..860
                     /locus_tag="AC16QF_17085"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR6450326.1"
                     /translation="AALYTNSTLAMDPRTGKIVWYFQHVPGETIDMEVGFERVLVDVE
                     GARLLFTVGKDGILWKLDRATGQYVAHVETIEQNIFEHVDPETGRVRYREDIRSAGVG
                     ETFSACPGIYGGHNWQAMAYSPQARSLVIPLHRLCADMVGRPVESKVGEGGFGGDSRS
                     YEMPGSNGNLGRLVAVDAATLEERWVHEQRAMFMTGALTTAGGLVFIGDLDRYVKAFD
                     VADGRLLWQARLGAPTHGYPVTYRAGGKQYLAVPTGMGVFRALTAVISPDIYQPANGQ
                     ALYVFELPN"
     gene            935..2644
                     /locus_tag="AC16QF_17090"
     CDS             935..2644
                     /locus_tag="AC16QF_17090"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR6450327.1"
                     /translation="MGGDAIDLGTMLLGLLGGLAIFLFGLDQLTNMLKVTAGARMGDL
                     LAKMTTNRFKGVLAGGVTTAVIQSSSVTTVLVVGFVSAGLMTLQQSIGVIMGAEIGTT
                     ITAQIIAFKITEYALVAVVVGFVMQAFFDNDLVKRYGTMVFGLGLVFFGMFMMGEATR
                     SLRDYPPFVDLLAQMDNRVLTILLSAGFTALIQSSSATTGVIIVLGSQGLISLEQGIA
                     LVFGANIGTCVTAVLASIGKKHRGLQVALVHVTFNVGGVLMWFAFIDELAAFSRWLSP
                     ASPGLVGADRLAEEIPRQIANAHTLFNVTNTLIFIWFTGAFAWAVTRLAPGKRSSGAG
                     IFEPRYLDSALVQTPALALDAARREIVRMGDHLVPMIRRCGTAVLRGSHADLARIREL
                     HGEVDELHVEIVRFLGRVSREEIAPRDSDELHQYLNIATLYESVGDIVETELVRVGAE
                     RIRHNVRVSADTENVMSALADKVVWALETATRAVDLEDASLARDVIAAKESVNRIADE
                     IGSRLMNRLVAEEPHRTSTYRLESQLIEHYKRVYYLAKRIAKLVASAESASGQDDAQA
                     VIA"
     gene            2774..3973
                     /locus_tag="AC16QF_17095"
     CDS             2774..3973
                     /locus_tag="AC16QF_17095"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6450328.1"
                     /translation="MDHLFDGLKVVDAASFLAGPGAGTVLSDFGADVIKVEPLHGDGY
                     RTLRGNYPIDYNWQLTSRNKRSIALDLDQEEGREVLRTLMADADVLLVNYIGDQLHRF
                     GLAYETVRQHNPGIVYAHLTGYGTEGPDAAKRGFDSTAWWARTGMMDLVRDPGQAPLI
                     GVPGFGDHSSAMSLFGAIMMGLYRRERTGEGSYVSTSLVANGVWANGMQLQGVIAGID
                     LGAIRQEKGWTNPFTSVYGSSDGRYVLLTVTNQAREWPALCKALGHQEWLEDERFADF
                     RTLFRNRVELIRLISEATETMTLAELTAALDAHGITYGVVSQMAEVVDDPHLRAAGII
                     VDTASPDESYAATIANPISMAEAPKRSHGRAPEIGEHSREILREHGFTDEQIDELERC
                     RVIGQHR"
     gene            3987..>4168
                     /locus_tag="AC16QF_17100"
     CDS             3987..>4168
                     /locus_tag="AC16QF_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HIR6450329.1"
                     /translation="MLNPEQVRAAALELNAAERERRQIRPTSVTYQGMTVEDAYAVQD
                     AWVKLKIDSGRRIVGR"
BASE COUNT          720 a         1267 c         1407 g          774 t
ORIGIN      
        1 acgcggcgtt gtacaccaac tccacgctgg ccatggatcc gcgaaccggg aagatcgtct
       61 ggtacttcca gcacgttccg ggtgaaacca tcgacatgga ggtggggttc gagcgggtgc
      121 tggtggacgt cgaaggcgca cggctgctgt ttaccgtcgg caaggacggc attctctgga
      181 aactcgatcg ggcgacgggg cagtacgtgg ctcatgtgga aaccatcgag cagaacatct
      241 tcgagcacgt cgatcccgag accggtcggg tgcgctatcg cgaggacatc cggtccgctg
      301 gcgtcgggga gacgttcagc gcctgtccgg gtatctacgg cggtcacaac tggcaggcga
      361 tggcttacag cccccaggcg cgaagcctcg tcattccgct gcatcggctc tgtgccgaca
      421 tggtcgggcg ccccgtggag tcgaaggtgg gagaaggcgg gttcgggggt gattcacgct
      481 cctacgagat gcctggcagc aatggcaacc tgggccgcct ggttgcggtg gatgctgcca
      541 ccctggagga gcgttgggtt cacgagcagc gggccatgtt catgaccggg gccctgacca
      601 cggcaggtgg tctcgtgttc atcggtgacc tggaccgcta cgtcaaggca ttcgacgtcg
      661 ccgacgggcg ccttctgtgg caggcacggc tcggcgcgcc cacacacggg tatcccgtca
      721 cctatcgtgc gggcgggaag cagtatctgg cggtgcctac cgggatgggc gttttcaggg
      781 cgctcaccgc ggtcatcagc cccgacatct accagcctgc caacggccag gcgttgtatg
      841 tcttcgaatt gccgaactga gcgctctttc agcgcctggg cactcgcggc caacgtgacg
      901 gggcggtcac gatgagtcgg gtgagatagc gagcatgggg ggtgacgcca tcgacctggg
      961 aaccatgctc ctcggccttc tcggcgggct ggcgattttt ctgtttgggc tcgatcagct
     1021 caccaacatg ctcaaggtca ccgccggtgc gcgcatgggt gacctgctgg ccaagatgac
     1081 gacgaaccgg ttcaaggggg ttctggccgg cggtgtgacc acggccgtga tccaatcgtc
     1141 gtcggtcacc acggtgctcg tcgtcgggtt cgtgtcggcg ggcctgatga cgctgcagca
     1201 gtccattggc gtcatcatgg gcgcggagat cggcaccacg atcaccgctc agatcattgc
     1261 cttcaagatc accgagtatg ccctggtcgc cgtggtggtg gggtttgtca tgcaggcgtt
     1321 cttcgacaac gatctggtca agcgctacgg gacgatggtc ttcggcctgg gcctcgtctt
     1381 cttcggcatg ttcatgatgg gcgaagcgac gcggtcgctg cgggattacc cccctttcgt
     1441 cgacctgctg gcgcagatgg acaaccgggt tctcacgatt ctcctcagcg ccggatttac
     1501 ggccctgatt cagagctcgt cggcgaccac gggcgtgatc atcgtgctgg gcagccaggg
     1561 gctcatttcc ctcgagcagg ggatagctct ggttttcggc gccaacatcg gcacctgcgt
     1621 gaccgccgtg ctggcttcca tcggaaagaa acaccgcggg ctccaggtcg ccctggtcca
     1681 cgtcaccttc aacgttggcg gggtgctgat gtggttcgcc tttatagacg agcttgccgc
     1741 cttctcgcga tggctgtctc ctgcgtctcc ggggttggtc ggtgcggatc gtctcgcgga
     1801 ggagatcccg aggcagatcg ccaacgccca tacgctgttc aacgtcacga atacgctgat
     1861 cttcatctgg tttaccgggg catttgcgtg ggcggtaact cggctcgcgc cggggaagcg
     1921 cagctcgggc gccgggatct tcgagccgcg ttacctggac tcggccctgg ttcagacgcc
     1981 ggcccttgcc ctggacgccg ctcggcggga gatcgtcagg atgggcgacc atctcgtgcc
     2041 catgatcagg cgatgcggga ccgcggtgct gcgcgggtcc catgcggatc tggccaggat
     2101 tcgggagctg catggagagg tcgatgagct gcacgtggag atcgtccgct ttttggggcg
     2161 cgtgtccagg gaggagatcg cgcctcgaga ctccgacgag cttcaccagt atctgaacat
     2221 cgcaacgctc tacgaaagcg tcggggatat cgtcgagaca gagctcgtgc gggtgggcgc
     2281 tgagcgtatc aggcacaacg tgcgggtcag cgcggacacg gagaacgtga tgagcgcgct
     2341 tgcggacaag gtcgtctggg cgctggagac ggcgacccgc gcggtcgatc tggaggatgc
     2401 gtcgctggct cgtgacgtga ttgccgccaa ggagtccgtg aaccggattg cggacgagat
     2461 aggctcccgg ctgatgaacc gactcgtggc cgaggagccc caccgcacct ccacctatcg
     2521 gctcgaaagc cagctgatcg agcattacaa gcgcgtgtac tatctggcga aacgcattgc
     2581 caagctggtt gcctcggccg agtcagcaag tggccaggac gatgcccaag ccgtgattgc
     2641 gtgacccgaa cgtcccgcgg cgtttcggtc attgcgcggg tgagatccgg cgatggattc
     2701 tgttctgacg gtggtcggca ttgatccccg ggcgcgggtc tctgacaatg gggcgagcag
     2761 ggagaatctg gctatggatc atctgtttga cggcctgaag gtcgtggatg ccgcctcttt
     2821 tctggccggg ccgggggcgg ggaccgtgct atcggatttc ggtgcggacg tgatcaaggt
     2881 cgaaccgctc catggcgacg gctaccgcac cctcaggggc aactacccca tcgactacaa
     2941 ctggcagctc acttcccgca acaagcgaag cattgccctc gacctcgacc aggaagaagg
     3001 acgggaagtg ctccgcactc tcatggccga tgcggacgtt ctgctcgtca actacatcgg
     3061 cgatcagctg caccgtttcg gcctggcgta cgagacggtc aggcagcaca atcccggaat
     3121 cgtctacgcc cacctcacgg gttacgggac cgagggcccg gatgccgcca agcgtggatt
     3181 cgattccacc gcttggtggg cgcgcacggg gatgatggac ctggtgaggg atccggggca
     3241 ggcgccgctg atcggcgtgc ccggctttgg cgaccactcc agcgccatgt cgttgttcgg
     3301 agccatcatg atgggcttgt accgtcgtga gcgaacgggg gagggcagct atgtctccac
     3361 ctcgctggtg gcgaacgggg tctgggctaa cggcatgcag ctgcagggcg tcatcgcggg
     3421 aatcgatctc ggcgccattc gccaggagaa gggatggacc aacccgttca cctccgtcta
     3481 cggatccagc gatggtcgtt acgtgctcct gaccgtgacc aaccaggccc gcgagtggcc
     3541 ggcgctgtgc aaagcgctcg gtcaccagga gtggctcgaa gacgagcggt tcgccgattt
     3601 cagaacgctg ttccggaatc gggtcgagct catccggctg atcagtgagg ccacggagac
     3661 catgacgctc gcagaactca cggcggccct ggatgcccat ggcatcacct acggcgtggt
     3721 cagccagatg gccgaggtgg tggacgaccc tcatcttcgc gccgcgggca tcatcgtcga
     3781 caccgcttct cccgacgaga gctacgccgc taccatcgcg aaccccatca gcatggccga
     3841 ggcccccaaa cgctctcacg ggcgggcgcc cgagatcggc gagcactccc gtgagattct
     3901 ccgcgagcac ggctttacgg acgagcagat cgacgagctg gagcgatgcc gcgtcatcgg
     3961 ccagcacagg tagtgacacc gcgcccatgc tgaaccccga gcaggttcgc gcggccgccc
     4021 tggagctgaa cgccgccgag cgggagcggc gccagatccg acccacgtcc gtcacgtacc
     4081 agggaatgac cgtagaagac gcctacgcgg tgcaggacgc gtgggtcaag ctgaagatcg
     4141 attccggcag acgcatcgtc gggcgcaa
//
LOCUS       DBNHLM010000369        18045 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_773971, whole genome shotgun sequence.
ACCESSION   DBNHLM010000369 DBNHLM010000000
VERSION     DBNHLM010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 18045)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18045)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18045
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_773971"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="AC16QF_17105"
     CDS             complement(<1..440)
                     /locus_tag="AC16QF_17105"
                     /inference="COORDINATES: protein motif:HMM:NF015557.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR6450330.1"
                     /translation="MPEVAVESSLHAINAHAVAVMLLTVSALYLFTRERIPMEVTSVG
                     LLAILAVGFSIFPFPGIEPTDFFHGLGHEALVAVCALMVVGQGLVRTGALEPVGRLLG
                     KIWSRAPFLSFLITLIVGAVLSAFVNNTPIVVLLLPILISVCLRT"
     gene            complement(572..2740)
                     /locus_tag="AC16QF_17110"
     CDS             complement(572..2740)
                     /locus_tag="AC16QF_17110"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HIR6450331.1"
                     /translation="MRKSSRTLFRSRSVMLAGTGLCIVAAWLASIAAAENPQLALSTP
                     TETADLLSPLDVHPRISLTIVEQLRHNHYLEKELDDQQSSHIFENYLGMLDGAKVYFT
                     AADVAAMERYRYKLDDALVRGNLVPAFEIFNRYQERLTDRLEFLLEELALGLKQIDFT
                     AEDQIEIERENAPWPANEAELDDLWRKRLKAAVLSMKLNGKELDEIQTLLTKRYTNRL
                     KQSRQTKSEDAFQLYVNAFATTYDPHTQYFSPRTSQNFNINMSLSLEGIGAVLRSEDE
                     YTSIVELVPAGPADKSGLVKPADRIVSVGQGDTGPLIDVVGWRLDDVVELIRGPKGSQ
                     VRLEIIPGDSEDDTSRVISITRNTVKLEEQAAQKKLLTMERNGQTQKIGVIEIPTFYV
                     DFKAIQQGDPNYKSTTRDVRLLIEELKAEGIDGLVVDLRNNGGGALQEADSLTGLFIK
                     SGPTVQVKSASRRPLVYSDDDNRAVWDGPLAVLVNRLSASASEIFAGAIQDYERGIII
                     GSQTFGKGTVQTLVPLNRGQLKITAAKYYRVSGQSTQHQGIIPDIEFPEIYDAERIGE
                     SALDDAMPWDMIQPAVYPRSHQIQPVLAELNSKHEERAGGDPDFQYFEALAQRSRENN
                     RRTHVSLNEKERVVQKKDEDTWRLDLENQLRVAKGEEPLESIDDLDDREADEDEAEDP
                     FGDPMIRESANILLDYIGLTRQIAYVEDLDKAAGPATAIQ"
     gene            3132..3809
                     /gene="bamJ"
                     /locus_tag="AC16QF_17115"
     CDS             3132..3809
                     /gene="bamJ"
                     /locus_tag="AC16QF_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BamJ family beta-barrel-assembly machinery
                     protein"
                     /protein_id="HIR6450332.1"
                     /translation="MGCQPAGDGTAADAQAAEESAELALDDQVAQASYGIGYTIASNL
                     DSQFAGSLNTEALMAGLQDSMEGRERRLSEAAIQTAIAALAQKQQEAVAARSADVLAA
                     GRAFLEENGRRDGVSTTASGLQYEIIATGDGPKPGASDTVSTHYHGTLIDGSVFDSSV
                     ERGQPASFAVNRVIPGWTEALQLMSVGSKWRLFIPPELAYGDRAAGSIPPNSTLIFEV
                     ELLAVDG"
     gene            3857..4126
                     /locus_tag="AC16QF_17120"
     CDS             3857..4126
                     /locus_tag="AC16QF_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450333.1"
                     /translation="MSGERRRPELGNTLTKRRMEPRRRGRLARTILLGTVAVAFSLFW
                     VARELELDTEELLGFLGASAVFVGLMVILGLVGAAAVLIFKRLGR"
     gene            complement(4142..4825)
                     /gene="lepB"
                     /locus_tag="AC16QF_17125"
     CDS             complement(4142..4825)
                     /gene="lepB"
                     /locus_tag="AC16QF_17125"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR6450334.1"
                     /translation="MTGRLHEHWVTWRGFALFLAIMLVFRSAVADWNQVPTASMFPSI
                     FVGDRIVIDKLAYGLRIPFTHQSITSWDDPHRGDVITFTSPEDERLLIKRVVGIPGDT
                     VALDGNRLVVNGQTARYTELTATERRALDGVADREYRLLRESLLGSQRLVMVRSSRVQ
                     DDDLPPTVVPPGQYLVLGDNRDNSRDSRSIGLVARDRILGKATAVAFSLNYDRYYAPR
                     VDRFFADLP"
     gene            complement(4834..5130)
                     /locus_tag="AC16QF_17130"
     CDS             complement(4834..5130)
                     /locus_tag="AC16QF_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HIR6450335.1"
                     /translation="MDKDQENAIEAAVFRRLLEHLDEHKDVQNIDLMNLAGFCRNCLS
                     KWYRAACEDAGVAMDYEAARERVYGMPYADWKSRYQAEATPEQLAGFESGRQQG"
     gene            5247..6026
                     /locus_tag="AC16QF_17135"
     CDS             5247..6026
                     /locus_tag="AC16QF_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR6450336.1"
                     /translation="MELLTNPEIWAAFVTLTILEIVLGIDNIIFISILTSRLPPEQRA
                     RGRVLGLGLAMLMRILLLLSLAGIMRLTTDLFEVMGQGISGRDLILIFGGAFLMAKAT
                     REVHNALEGEAHEGQGAGAPTFAGVLLQIALIDMVFSLDSVITAVGLVDEVPVMVAAV
                     ISAVLVMMVAAGPISDFVEDHPTVKMLALAFLLLIGLTLMGEGIEIHTPKGYIYFAMA
                     FSFGVEILNIRAKKRRKDHVMLRKTQLADLVASRSEKGRPD"
     gene            complement(6039..6815)
                     /gene="yaaA"
                     /locus_tag="AC16QF_17140"
     CDS             complement(6039..6815)
                     /gene="yaaA"
                     /locus_tag="AC16QF_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HIR6450337.1"
                     /translation="MLAVLSPAKTLDFETPSPTRKHSLPVFLDESKQLVETLRTQSPA
                     DLAQLMSMSTKLADQNYQRYTDWQTPFTAGNAKQAALAFKGDVYLGLEPGTFSERDFS
                     WAQKHIRILSGLHGILKPLDLIQPYRLEMGTRLKTRRGADLYAFWGQKITDELNAALA
                     EQKRPVLINLASNEYFDAVDPGRLNARIVTPTFKDLKNGRYRFMSFFAKRARGMMAGY
                     LVKNRVNTLKGLREFDWGGYYYSEDLSRGDDWVFLRDKPA"
     gene            6867..7586
                     /locus_tag="AC16QF_17145"
     CDS             6867..7586
                     /locus_tag="AC16QF_17145"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR6450338.1"
                     /translation="MRIIGRTDRGRVRGSNQDAWACDAELGVAVLADGMGGLTGGEVA
                     SRAAVECVLGHLAGVPCIDADSVREAIAAANGRVLELSREQADGDMGTTLVVWARQAP
                     GRYLLGHVGDSRAYRLSGAVLEPLTRDHSVVQAMVDDGRLSEADALCAPDRNLITRAV
                     GLEVDVFADVVELEYEAEDAFLLCSDGLTDLLAPDELAEICDGHLGPAMRDLPDSLVN
                     AANAAGGYDNITVVLVYNCDP"
     gene            7606..7920
                     /locus_tag="AC16QF_17150"
     CDS             7606..7920
                     /locus_tag="AC16QF_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HIR6450339.1"
                     /translation="MSIQDRINERLQSRFDLHHLEVVNESGGHNVPPGSETHFKVILV
                     SPQFEGLRLIQRHRLVHETLAEELASGVHALAVHTYTDAEWRSRFGSAPMSPPCEGGG
                     RA"
     gene            7896..8855
                     /gene="trhO"
                     /locus_tag="AC16QF_17155"
                     /gene_synonym="yceA"
     CDS             7896..8855
                     /gene="trhO"
                     /locus_tag="AC16QF_17155"
                     /gene_synonym="yceA"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent tRNA uridine(34) hydroxylase
                     TrhO"
                     /protein_id="HIR6450340.1"
                     /translation="MRGRRAGVTSVVTFYKFVRLLDPSGLRDELDGMACELALKGTIL
                     LAGEGINGTVTGNAVALQKFLGALERLPEFSGLAVKYSSAAAGNPVFHRLKVRVKPEI
                     VSFGVSGLDPGGRTGTHVGAEEWNELLDDPEVTVVDTRNDYEVAIGSFPGAVNPATQS
                     FRQFADFVADHLDPDRDTRVAMFCTGGIRCEKASAYLLERGFAEVYQLDGGILNYLET
                     VKGEANRWEGECFVFDQRVSVDRHLREGSYEQCFACRRPLSTDDLQSPDYRPGISCPH
                     CAQEQPEARRAALAERARQVALAARRGEAHIGVRQGSQGRTNG"
     gene            complement(8852..9442)
                     /locus_tag="AC16QF_17160"
     CDS             complement(8852..9442)
                     /locus_tag="AC16QF_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HIR6450341.1"
                     /translation="MIALIAGVSPASARSKILVMGDSISAAYGIQRDAGWVALLDRRL
                     SETASPWQVVNASISGETTGGGLARLPKALEAHDPEIVIIELGGNDGLRGYPVSRIRE
                     NLDKLVELAVTGGRRVLLLGMRIPPNYGPRYTRAFEDMYRDVAEAHGVRFVPFILEAV
                     ALNPGLMQDDGIHPTAEAQQKLLDSVWPELQQVLDG"
     gene            9462..10136
                     /locus_tag="AC16QF_17165"
     CDS             9462..10136
                     /locus_tag="AC16QF_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6450342.1"
                     /translation="MTNQPVIRTEGVSKSVPTSEGELSILESIDLEINAGETVAIVGA
                     SGSGKTTLLGILAGLDLPTSGRVYLVGEELTALDEEQRARVRGEQVGFVFQTFQLLRS
                     LTALENVMLPGELRGDGDAQPEALRVLDKVGLGARTGHYPTQLSGGEQQRVAIARAFA
                     SRPNVLFADEPTGNLDTATGQRIIDLLFELNAEYATTLVLVTHDDRLASRCERVVEIV
                     AGRITS"
     gene            10133..12625
                     /locus_tag="AC16QF_17170"
     CDS             10133..12625
                     /locus_tag="AC16QF_17170"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6450343.1"
                     /translation="MSIKLVLRLAWRDWRGGELGLLLVALMLAVGTVTAVSLFVDRLH
                     QALIDESADFLAADRRISGSRPIPDEFRLAASAKGVEHADTMSFPSMVFVGDDNSQLV
                     SVKAVGAGYPLRGVLKIADEPFAPGVPTSDIPAPGEVWLDARLFPALGIRPGDSIEVG
                     VAPLKVAKVLTSEPDRGGSMYDLGPRLLMRLDDVPATQVVQPGSRIAYRLLLRGAESD
                     LESLKAELPLAPNYRWQGIRESSPSIGSALARAESFLLLGGLLGVLLAGVAVALSAHR
                     YALRHYDHVGILKTLGATPNQILSGYLMLLLAVGSAAVVLGLTGGAALHLGIVALLQS
                     LIPVDLPMPSARPFLLGAATGLICAVAFALPPLLHLKNVSPMRVIRRDLGAAPPSQYL
                     SYGAAVAGSVGLLIWYSGSASLTAWTILGTVVVLLVFGALAMLLLRGGRAVGMQAGSG
                     WRLALAGLQRRRRENVAQILIFGLAIMLLLILVLLRSSLLDEWQGQVPEDAPNHFVLN
                     IVPHEVDELATMLDDFTGSHGGLFPMIRGRIVAANGVDADAWERDHQPVDAPGPRLQS
                     ERNLTWAADLPDDNQVVKGRWWSPDEARALVSLEDEYAQSLGLDVGDRLRFDIAGRFV
                     EVEVASLRRLDWESMRPNFFIIFAPQVLSDFPATYMTSFHLERDEKPFLNRFLARFPT
                     VTVIEVDAIIAQVQSIILRVTQAVELVLALVVASGCLVLVASIQASRDGRMSELALVR
                     TLGGSRRLIAGSLVAEFAALGLFAGTVAVVGAEATVAILQSQVFELPVRLHPWLWLLG
                     PVIGSVLILIVGTLGTRGLISTPPVMVLRGLD"
     gene            12739..13590
                     /gene="ttcA"
                     /locus_tag="AC16QF_17175"
     CDS             12739..13590
                     /gene="ttcA"
                     /locus_tag="AC16QF_17175"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HIR6450344.1"
                     /translation="MRKHGYDMNKLHRKLRRKVGQAIADYGMIEEGDRVMVCLSGGKD
                     SYTLLDILLNLQRSAPVAFEIVAVNLDQKQPGYPADVLPGYLEGLDVSYHILEQDTYS
                     IVKAVTPAGKTTCPVCSRLRRGILYNFAASIGASKIALGHHLDDVVETLFLNMFHGAR
                     LKAMPPKLKSDDGRNVVIRPLYYCRERDIARWAEHREYPIIPCNLCGSQENLQRQVIK
                     SMLARWDAEQPGRVENIARSLSAVVPSHLGDRGLFDFAGLSSDDGVEKLQLLDLTPRC
                     VNAMPPR"
     gene            complement(13553..>18045)
                     /locus_tag="AC16QF_17180"
     CDS             complement(13553..>18045)
                     /locus_tag="AC16QF_17180"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6450345.1"
                     /translation="AGRLRPALTKGLLTTATGSKEAPGDQHQLLYRFAHPRVRSLVYA
                     EMAQEAKSTIHFAIADECKRGHRPAGRTLLQIAHHLNAAADPIGLDKDKRLEIAHYNL
                     LAAKESIGQGAFQPAYKFCRSGLSLLSELPDEPIFIELCQSAAEAAFYCSDFEQLDRV
                     IASIPHNTSALDEIRVRAAVVQNHLGAAREQAFDALESLGYVRPATGINPIGQAFRRL
                     GRKRAPDAAPKPAPVLEDIRLQHTFRLLGYLLHAGYHLGSEGLDALTDDMLSEAARHG
                     YSAEIAFAFAARAVSAVADHRIGIAQRYAAEARALAGQFSGDAFSIRAETLLNGLVDP
                     WSGGLDDTLGGLSRNIDASMACQDYEFAATATAFYATNGLLRGLDLASLRRELNEQIS
                     RLSGLQHVTGVNISLFVQQIIGSLLGHAEADGESDHFRELSISNSEDAVAHGYVYVLR
                     LYYAVLFDDFRGAANILELAEQFGPALAGSPLLIVWKFADALVNLRSGNTAGLRRARA
                     SLRELKQWHQDGADHAEPKVILLQAELNWQRGATTQAIECYEVAADKARRLGLANDEA
                     LAYELAARACESNGRADFAKLFARNAHQAYLRWGANAKASHLEREFPSMLDDALHPAT
                     VIGDLTDLTVRDFSSMSIRSSLDGSEINANVLDTTTVLRAAQTISSEIVLDRVLTKLL
                     RLALEHAGAQKACMLLAHDGRLFVEAIASVDGGPTRRESPATPLEASDDVPESIVHFV
                     ARTKQALVLGDATAEDVFTQDPYIVRKQPLSVLCVPIVHRNEITGILYVEHRWLTGVF
                     TAQRVEVLALLASQAAISIENARLYADLHDTQEQYRALYDNAIEGLFRIDPDGALLSA
                     NPTLARILGFDDVKQLLAEYRDLIDRVFLSREDASHFLTNLEEHGLLNVFEAQGVTRN
                     GRTFWMALTARLSHDPEQGAYIDGSLVDISERMKREQADKHRQIAEAATQAKSEFLAN
                     MSHEIRTPMNAIIGFSKLALETGLDPKQHEYLSSIRAASENLLSLVNDVLDFSKIEAG
                     KLTLEHRPFKLADTLKEVERLFRTEVRKSNLELNFDDNTAAHAEFPGDGVIIGDPLRL
                     QQVLVNLVSNALKFTEEGSISVTAQVCPCEAGELRVEFAVRDTGIGIKPEHQARLFES
                     FEQAETSTTRRYGGTGLGLTICKRLVSLMGGEMSVASVPDQGSCFTFTAVFGLRDAQD
                     LMPADVRQRRRNLRALNRREFLVAEDNPINQQLSLEFLQRTGARVDIAQTGREAVERA
                     TSKAYDAILMDIHMPELDGLQACRILREQQITTPIIAVSADAIEASQQAALEAGCNAY
                     VSKPIDFDLLLAELSRVLPEQPADAASVDAPASAPAEHEASTTPGELPEIQLPGIDLD
                     TAIRGHNGNVRLMLKLMGDFGRYYGDAGAKMRAHVTDNHLEEAERLAHNLHGVAGSFG
                     ADRLKQASKTLELALAKGDESNLLGLVQSFEVAMNEVLESAETLANHPFTEAASRSRN
                     EG"
BASE COUNT         2902 a         5647 c         6072 g         3424 t
ORIGIN      
        1 gtgcgcaggc agacgctgat gagaatgggc agcagcagaa ccacgatggg cgtgttgttc
       61 acgaacgccg acagcacggc gccgacgatg agcgtgatca gaaacgacag gaacggagcg
      121 cggctccaga tcttgccgag caatcgaccc acgggctcga gcgcccctgt ccggaccagg
      181 ccctggccga ccaccatcag ggcgcagacg gccaccagcg cttcgtggcc gagcccgtga
      241 aagaaatcgg tgggctcaat gcccgggaag ggaaagatgg agaatccaac tgcgaggatg
      301 gcgagcagac cgacgctggt cacctccatg ggtatgcgct cgcgggtgaa gagatagagc
      361 gcgctgaccg tgagcagcat aacggcgacc gcgtgagcgt tgatcgcatg caggctgctt
      421 tctacagcca cttcaggcat cgtgcgtctg acaagaaagc aacttagatg acgtccacga
      481 gaaggtcgga gaagtgtacg tctattctgg ggtcaaagcc tgcagaaatc tctcaatatt
      541 ggcccggagc gtcgggtcgc ccggcgattt tctactggat ggccgtcgcg ggcccggcag
      601 ccttgtcgag gtcttccacg taggcgatct gccgggtgag gccgatgtag tcgagcagaa
      661 tgttggcgct ctcgcgaatc atgggatcgc cgaaaggatc ctccgcttcg tcctcgtccg
      721 cctcccgatc gtccaggtcg tcgatggatt ccagcggctc ttctcctttg gcgacccgga
      781 gctgattctc cagatccagg cgccacgtgt cctcgtcctt cttctgcacc acgcgctctt
      841 tctcgttcag ggagacgtgg gtgcgacggt tgttctcgcg gctgcgctgg gcgagggctt
      901 cgaagtactg gaagtcagga tcgccgcccg cacgctcttc gtgcttggag ttgagctccg
      961 caaggaccgg ctgaatctga tgtgatcgcg ggtacacggc gggctggatc atgtcccagg
     1021 gcatggcgtc atcgagggcg ctttcgccga tacgctccgc gtcgtatatc tccgggaact
     1081 cgatgtcggg aatgatgccc tggtgctggg tgctctggcc ggacacccga tagtacttgg
     1141 ccgcggtgat cttcagctga ccccggttca aggggaccag ggtctgcacc gtgcctttgc
     1201 cgaaggtctg gctgccgatg atgatgccgc gctcgtagtc ctgtatggcg cccgcgaaga
     1261 tctccgaggc cgaagcggag agacgattga ccagcaccgc gagggggccg tcccacacgg
     1321 cccggttgtc gtcgtccgaa tagaccaggg gccgtcgcga ggcggatttg acctgcacgg
     1381 tgggcccgga cttgatgaac agcccggtga gcgaatccgc ttcctgcagg gcgccaccgc
     1441 cgttgttgcg aagatcgacg acgaggccgt ctatgccctc cgccttgagc tcctcgatca
     1501 gcagccgcac gtctcgcgtg gtgctcttgt agttgggatc gccctgctga atggccttga
     1561 agtcgacata gaacgtcggg atctcgatga cgcctatctt ctgggtctgg ccgttgcgct
     1621 ccatggtgag cagcttcttc tgggcggcct gctcctcgag cttcacggtg ttccgcgtga
     1681 tgctgatcac gcggctggtg tcgtcctccg agtcgccggg gatgatctcc agcctcacct
     1741 gggagccctt ggggccacgg atgagctcca cgacgtcgtc gagccgccag cccaccacgt
     1801 cgatcagcgg tccggtatcg ccctggccca cgctgacgat ccggtcggcc ggcttgacca
     1861 gcccggattt gtccgcgggc ccggcgggga cgagctcgac gatggacgtg tactcgtcct
     1921 ccgagcgcag aacggcgccg atgccttcca gggaaagcga catgttgatg ttgaagttct
     1981 gcgacgttcg cggcgagaag tactgggtgt gcgggtcgta ggtggtcgcg aaggcgttca
     2041 cgtagagctg aaacgcatct tcgctcttgg tctgccggct ctgcttgagc cggttggtgt
     2101 agcgcttggt cagcagggtc tggatctcgt cgagctcttt gccgttgagc ttcatgctga
     2161 gcaccgctgc ttttagccgc ttgcgccaca gatcatccag ctcagcctcg ttcgcgggcc
     2221 acggggcgtt ctcgcgctcg atctcgatct ggtcctcggc ggtgaagtcg atctgcttca
     2281 gacccagcgc cagctcttcc agcaggaact ccagccggtc ggtcaggcgc tcctggtacc
     2341 ggttgaagat ctcgaaggcg ggcacgaggt tgccccgcac cagagcatcg tccagcttgt
     2401 agcgatagcg ctccatcgcc gccacgtcgg cggcggtgaa gtacaccttg gcaccatcca
     2461 gcatgccgag atagttctcg aagatgtggc tcgactgctg gtcgtccagt tccttttcga
     2521 ggtagtggtt gtgacgcagc tgctcgacga tggtgaggct gatccgcggg tgaacgtcca
     2581 gcggcgacag aaggtcggcg gtctctgtcg gggtagacag cgccagctgc ggattctcag
     2641 ccgctgcgat ggaggcgagc caggccgcaa cgatgcataa gccggtgccc gccagcatga
     2701 ccgagcggga ccggaagagg gttctagagc ttttgcgcat aacgtgagtg atctgatggt
     2761 gccctgaatg aaaatgtggg tcgcattggc ggcgtcgggt ccaggccctg gccttcgtat
     2821 tgttcgtaac tcagtcgccg gggatctgca gattttcgcc ggtccggtcg gcaaactcgc
     2881 ttctgccacc cgtttcggta caaactgtgc cgcaactgat cgctacttct gtagatacca
     2941 tttctcctgg gtggttcccc caaaccactc cagcgagact atctaaatgc ccgcatgcgt
     3001 actgttgcgg gaatgtaacc cggtgtctgg gtgtgggcaa ccggggcagg gagcccaaat
     3061 tgatggaatg ccgaacggac atgagtactt ggtcaagaaa tttcgcatct ttgctggtgg
     3121 tgctgctggc gatggggtgc cagccggccg gggacggcac cgcggcggat gcgcaggcgg
     3181 cagaggaatc cgcagagctg gcgctggacg atcaggtggc ccaggccagc tacgggatcg
     3241 ggtacaccat cgcgagcaat ctggactccc agtttgcggg ctccctgaac accgaggccc
     3301 tgatggcggg ccttcaggac agcatggagg gtcgggagcg ccggctttcc gaggcggcga
     3361 tccagaccgc catcgcggcg ctggcacaga agcagcagga ggcggtggcc gctcgctccg
     3421 cggatgtgct ggccgccggc cgggcgtttc tggaggaaaa cgggcgccgg gacggcgtca
     3481 gcacgacggc gtcgggactg cagtacgaga tcatcgcgac cggcgacggt cccaagccgg
     3541 gcgcctcgga caccgtgagc acgcactatc atggcaccct gatcgacggc agcgtgttcg
     3601 acagctccgt ggaacgcggc cagccggcaa gctttgccgt caaccgggtg attccgggct
     3661 ggacggaagc gctccagctg atgtcggtgg gttccaagtg gcgtctgttc attccgcccg
     3721 agctcgcata cggagatcgg gcagcgggct ccatccctcc caactcgacg ctgatcttcg
     3781 aggtcgagct cctggccgtt gacggttagc acgcacgtgg ctcatcgcga gcgggtctgg
     3841 acggcggccg cttcccatga gcggtgagcg ccgccgccct gagctcggca acacgctgac
     3901 caagcggcgc atggagcccc gcaggcgcgg gcgccttgcc cggaccatac tgcttggcac
     3961 ggtggcggtg gcgttctcgc tgttctgggt cgctcgggaa ctggagctcg acaccgagga
     4021 gctgctgggt ttcctgggtg ccagcgccgt gttcgtcggg ttgatggtga tactcgggct
     4081 ggtgggcgcg gccgccgtcc tgatcttcaa gcgcctcggc cgctgacggc ccgctcacgg
     4141 atcagggaag atcggcaaaa aagcggtcaa ccctcggcgc gtagtaccga tcgtagttca
     4201 gggagaaggc gacggccgtt gccttgccga gaatccgatc ccgggccacc agaccgatgc
     4261 tgcgcgagtc ccggctgttg tctcgattgt cgccgagcac caggtattgg ccgggaggca
     4321 cgacggtggg cggcaggtcg tcgtcttgca cacgggagct tcgcaccatc accagtcgct
     4381 ggctgcccag cagagattcc cgcagcagcc gatactcccg gtcggcgacg ccgtccagcg
     4441 cccggcgttc ggtggccgtc agctcggtgt agcgggcggt ctggccgttg acgacgagcc
     4501 ggttcccgtc gagcgcgacg gtgtcgccgg gtatcccgac aacgcgcttg atcagcagcc
     4561 gctcgtcttc gggcgaggtg aaggtgatca cgtcaccgcg atgggggtca tcccagctgg
     4621 tgatggactg gtgcgtaaaa ggaattctca gcccgtaggc cagcttgtcg atcacgatgc
     4681 gatcgccgac gaagatggag ggaaacatgg aagccgtcgg cacctgattc cagtctgcca
     4741 cggcggagcg aaagaccagc atgatggcca gaaagagggc aaagcctcgc caggtgaccc
     4801 agtgctcgtg cagtcggccg gtcacgctcc tgatcagccc tgctgccggc ccgattcgaa
     4861 gccggccagc tgctcgggcg tcgcctcggc ctggtagcgg gacttccaat ccgcgtaggg
     4921 cataccgtag acccgctccc gcgccgcttc gtagtccatg gcgacgccgg cgtcctcgca
     4981 ggcggcccga taccacttcg acaggcagtt gcggcagaaa ccggccaggt tcatcaggtc
     5041 gatgttctgc acgtccttgt gctcgtcgag atgctccagc aggcgccgaa acaccgcggc
     5101 ctctatggcg ttttcctgat ctttgtccac gacggatccc aaagttgaca cgcgttggcc
     5161 gcattctacc gaaaacttgc ggcctgggtg cttcggcaga aatcctgtgc ttcaatgccc
     5221 gcaagtcaac cggctgacgg cagattgtgg agcttctcac caacccggag atttgggccg
     5281 cgttcgtcac gctgacgatc ctcgagatcg tgctcgggat cgacaacatc atcttcatct
     5341 ccatactcac cagtcgcctg ccgcctgagc agcgtgcccg cggccgcgtg ctcggtcttg
     5401 gcctggccat gctcatgcgc attctgctgc tgctctcgct tgccgggatc atgcggttga
     5461 ccacggacct tttcgaggtg atgggccagg gcatatcggg ccgcgacctg atactgatct
     5521 tcggcggcgc cttcctcatg gcgaaagcca cccgggaagt tcacaacgcc ctggaaggtg
     5581 aagctcacga aggtcagggc gccggcgcgc cgacgttcgc gggcgttctg ttgcagatcg
     5641 ccctgatcga catggtcttc tcgctggatt cggtgatcac ggcggtaggc ctggtggacg
     5701 aggtgccggt gatggttgcc gccgtgatct ccgcggtgct ggtgatgatg gtcgccgcgg
     5761 gaccgatcag tgatttcgtg gaagatcacc caacggtgaa gatgctcgcg ctggcgtttc
     5821 tgcttctcat cgggctcacg ctgatgggcg aaggcatcga gatccacacg cccaagggat
     5881 acatctactt tgccatggcg ttttcgttcg gagtggagat cctcaacatc cgggccaaga
     5941 aacgcaggaa ggatcacgtg atgctgcgca agacgcagct tgccgatctg gttgcctcgc
     6001 ggtcggagaa gggccggccg gattagccgg ccgccggctc aggcgggctt gtcacgcaga
     6061 aagacccaat cgtctccccg ggacagatcc tcggaatagt agtagccgcc ccagtcgaac
     6121 tcgcgaagcc ctttgagcgt gttgaccctg ttcttcacca ggtagccggc catcattccc
     6181 cgcgcccgct ttgcgaagaa ggacatgaag cggtacctgc cgttcttgag gtccttgaag
     6241 gtgggcgtga cgatccgcgc gttcaggcgg cctgggtcga cggcgtcgaa atactcgttg
     6301 gaggcgaggt tgatcagcac cgggcgtttc tgctcggcca gggcggcgtt cagctcgtcg
     6361 gtgatcttct gaccccagaa agcgtagaga tcggccccgc ggcgggtctt gagccgggtg
     6421 cccatctcca ggcggtacgg ttgtatgaga tcaagtggct tcaggattcc atgcaggccg
     6481 gacagtatgc ggatgtgctt ctgtgcccag ctgaagtcac gctcggagaa cgtgcccggt
     6541 tccaggccga ggtacacgtc gcccttgaac gccagggcgg cctgcttggc gttgcccgcc
     6601 gtgaacggag tttgccaatc ggtgtagcgc tggtagttct gatcggcgag ctttgtgctc
     6661 atgctcatca gctgtgccag gtctgccggg gactgagttc tgagcgtctc caccagctgc
     6721 tttgactcgt cgagaaaaac tggcaggctg tgcttgcgcg tgggggaggg cgtctcgaaa
     6781 tctaatgttt ttgccgggga tagaactgcc agcatttggc cggaggctcc tggattgatt
     6841 cgggtgccaa gtgtaacgga attcgcgtga gaatcatcgg acgcacggat cgcggccggg
     6901 tgcgcggcag caaccaggac gcctgggcgt gcgacgcaga gctcggcgtc gccgtgctcg
     6961 ccgacggcat gggggggttg accggcgggg aggtggcaag ccgggcggcg gtggagtgcg
     7021 tcctgggaca cctggccggc gttccctgca tcgatgccga cagcgtcagg gaggccatcg
     7081 ccgcggcgaa cggcagagtt ttggaacttt cccgcgagca ggctgatggc gacatgggaa
     7141 cgaccttggt ggtctgggcc cggcaggcgc ccggccgcta cctgctcggc cacgtgggcg
     7201 actcgcgcgc gtaccggctg agcggcgccg tcctcgagcc gcttacccgc gatcacagcg
     7261 tcgtgcaggc catggtggat gatggccggc tgagcgaagc ggacgccctg tgcgcgcccg
     7321 atcgcaatct gatcacccgg gcggtcgggc tcgaggtgga tgtgttcgcg gacgtggtgg
     7381 agctcgagta cgaagcggaa gatgcgtttc tgctctgctc cgatggcctc accgatctgc
     7441 tcgctccgga cgagctggcg gaaatctgcg acggccatct agggccggcc atgcgggatc
     7501 ttccggacag cctcgtgaat gccgcgaacg cggcgggggg atacgacaac atcaccgtgg
     7561 tgctcgtcta taattgtgac ccctgatggt ttgcaactgc tgtcgatgtc gatccaagac
     7621 cggataaacg agcgtcttca atcccgcttt gatctgcacc acctcgaggt ggtcaacgaa
     7681 agcggcgggc acaacgtgcc gcccgggtcg gagacccact tcaaggtgat tctcgtctcc
     7741 ccgcagttcg aaggcctgcg cctgatacag cgccaccggc tggtccacga gacgctcgcc
     7801 gaggagttgg cctccggcgt gcacgcgctg gccgttcaca cctacaccga cgccgagtgg
     7861 cgctcgcgct tcggcagcgc gcccatgtcg ccgccatgcg agggcggcgg gcgggcgtga
     7921 ccagcgtcgt cacgttctac aagttcgtgc gcctcctcga tccctcgggg ctgcgcgacg
     7981 agctcgatgg catggcttgc gagctggcgc tgaagggaac gatcctgctc gccggggaag
     8041 gcatcaacgg aacggtcacg ggaaatgccg tggctctgca gaaatttctg ggcgccctcg
     8101 agcggttgcc ggaattctcc ggcctggcgg tcaagtattc ttccgcggct gcgggcaatc
     8161 cggtgttcca ccggctcaag gttcgcgtca agccggagat cgtcagcttc ggcgtttctg
     8221 gcctcgaccc gggtggccgg accggcaccc atgtgggtgc cgaggaatgg aacgaactgc
     8281 tcgacgaccc cgaggtgacc gtcgtcgaca cgcgcaacga ctacgaagtt gcgatcggct
     8341 cctttcccgg tgccgtcaac ccggcgacgc agagcttccg ccagtttgcg gacttcgtcg
     8401 ccgaccacct cgaccccgac cgcgacacca gggttgccat gttctgcacc ggtggcattc
     8461 gttgcgagaa ggcctctgcc tatctgctcg aacgtggctt cgccgaggta tatcagctcg
     8521 acggcggcat cctcaattac ctggagacgg taaaagggga ggcgaatcgt tgggaaggcg
     8581 aatgctttgt gttcgaccag cgcgtctccg tcgaccggca tcttcgggag ggctcgtacg
     8641 agcagtgctt tgcctgtcgg cggccgttgt cgaccgatga cctgcaatcg cccgactacc
     8701 gccccggcat ttcctgcccc cattgcgccc aggagcagcc cgaggcccgg cgagccgcgc
     8761 tcgccgagcg ggcccggcag gtggcgctgg ccgctcgccg tggtgaggcg cacatcggcg
     8821 tccgccaggg gagccagggg agaacgaacg gctagccgtc cagcacctgc tgcagctcgg
     8881 gccagacgga atccagcagc ttttgttgcg cctcagccgt tggatggatc ccgtcgtcct
     8941 gcatcaggcc cgggttcaag gccaccgcct ccaggatgaa aggcacgaaa cgcacgccgt
     9001 gggcctcggc gacgtcgcga tacatgtcct cgaaagcgcg cgtatagcgg ggcccgtagt
     9061 tgggcgggat cctcatgccg agcaggagca ccctgcgccc gcccgtcacg gccagctcca
     9121 cgagcttgtc gagattttcc cgtatgcggc tcaccgggta gccgcgcaga ccatcgttgc
     9181 cgcccagctc gatgatgacg atctcgggat cgtgagcttc gagggccttg ggcaggcgag
     9241 ccagccctcc gcccgtggtt tcgccgctga tgctggcgtt gaccacctgc cacgggcttg
     9301 cggtttcgct gagtcgccgg tccagcaggg cgacccagcc ggcgtcgcgt tgaataccgt
     9361 acgcagcgct gatactgtcg cccatcacca gaattttcga ccgcgcgctg gccggcgaga
     9421 cgcccgcgat cagcgcaatc atcagggtca ggaataccga tttgacgaac cagccggtaa
     9481 ttcgtaccga aggggtgtcc aaaagcgtgc cgacgagtga gggggaactc tccatcttgg
     9541 agagcatcga cctcgaaatc aacgcgggag aaaccgtcgc catcgttggc gcctccggct
     9601 cgggcaagac cacgcttctg ggtattctcg ccgggctcga cctgccgacc agcggccggg
     9661 tctatctcgt cggtgaggag ctgacggccc ttgacgagga gcagagggcg cgggttcgcg
     9721 gcgagcaggt cgggttcgtc tttcagactt ttcagcttct gcgcagcctg accgcgttgg
     9781 agaacgtcat gcttcccggc gagctgcgcg gcgatgggga tgcgcagccc gaagctctgc
     9841 gggtgctgga caaggtcggg ctcggcgcgc gcacggggca ctatccgact cagctttccg
     9901 gcggtgagca gcagcgggtt gccatcgcga gggctttcgc ttcccggccc aacgtgctgt
     9961 ttgcggatga gcccaccgga aatctggata ctgccacggg ccagcgcatc atcgacctgc
    10021 tcttcgagct caacgccgag tacgccacca cgctggttct cgtcacccac gatgatcgtc
    10081 tcgcctcccg ttgtgagcgg gtggtggaga tcgtggcagg caggattacc tcgtgagcat
    10141 caagctggtg ctgcggctgg cctggcgcga ctggcgtggc ggggagctcg gtctcctgct
    10201 cgtcgccctg atgctggcgg tggggacggt aacggccgtg agcctcttcg tagaccgact
    10261 gcaccaggcg ctcatcgatg aatccgccga ctttctcgcc gcggacagac gcatcagcgg
    10321 cagccgcccg atcccggacg agttccggct tgcggcgtcg gccaagggcg tggagcacgc
    10381 tgacaccatg tccttcccct ccatggtttt cgtcggtgac gacaacagcc agctggtcag
    10441 cgtcaaagcc gttggagcag gctacccgct gcgaggcgtc ctgaagatcg ccgatgagcc
    10501 ctttgcgccc ggcgtgccga catctgacat accggcgccg ggggaggtct ggttggatgc
    10561 gcgcctcttc cctgcgctgg gaattcgacc gggggactcg atcgaggtag gcgttgcccc
    10621 gctcaaggtc gcaaaggtgc tgacctcaga gccggatcgg ggcggcagca tgtacgacct
    10681 ggggccgcgc ctgctcatgc ggctggacga cgtgcccgcc acgcaggtcg tgcagcccgg
    10741 cagccgcatt gcctatcgcc tgctgctgcg tggtgcggaa tccgatctcg agagcctcaa
    10801 ggccgagctg ccgctcgccc ccaactaccg ttggcagggc attcgcgaaa gcagtccctc
    10861 catcggctcg gccctggctc gcgcggagag ctttctgctg ctcggtggtt tgctcggcgt
    10921 tctgctggcc ggcgtggcgg tcgcattgag cgcgcatcgc tacgcgcttc gccactacga
    10981 tcatgtgggc attctgaaaa ccctgggcgc gacgccgaat cagatccttt ccggctacct
    11041 catgctcctg ctcgccgtag gaagcgccgc cgtcgtgctg ggcctgaccg ggggcgcggc
    11101 cctgcacctg gggatcgttg ccttgctgca gtctctgatc ccggtggacc tgccgatgcc
    11161 gagcgcgcgc ccgttcctgc tgggcgccgc cacgggcctc atctgcgcgg tggcgtttgc
    11221 cctgccgccc ttgctgcatc tcaagaacgt ctctcccatg cgagtgattc gcagggacct
    11281 cggtgctgcc ccgccgtccc agtatctgag ttacggggct gcggtggcgg gaagcgtggg
    11341 cctgctgatc tggtacagcg gcagcgcgtc cctcacggcg tggaccatac tcggcaccgt
    11401 ggtcgtcctg ctggtattcg gggctctcgc gatgctgctg ctgcgcggcg gtcgcgcggt
    11461 cggcatgcag gcgggcagcg ggtggcggct ggcgctggcc ggtctgcagc gtcgccgtcg
    11521 ggagaacgtg gcccagatac tgattttcgg tctcgccatc atgttgctgc tgatcctggt
    11581 cctgcttcgc tcgtcgttgc tcgacgagtg gcagggacag gtgccggaag acgcgcccaa
    11641 ccatttcgtt ctcaacatcg ttccgcacga ggtggacgag ctcgcgacca tgctggacga
    11701 ttttacgggc agccacgggg gcctgtttcc catgatccgc ggccgcatcg tggccgccaa
    11761 cggggtggat gccgacgcgt gggagcggga ccatcaaccg gtggacgcgc cgggcccgcg
    11821 acttcagtcg gagcgaaatc tcacctgggc cgccgacctg ccggacgaca atcaggtggt
    11881 caaaggacgc tggtggtcgc cggacgaggc ccgggccctg gtttccctcg aggacgagta
    11941 tgcccagagc cttgggctgg acgtcggcga ccggctgcgc ttcgacattg ccggtcgctt
    12001 cgtggaggtg gaagtcgcca gcctgcggcg gctcgattgg gagagcatgc gccccaattt
    12061 cttcatcatc ttcgctcccc aggtgctcag cgatttcccc gctacctata tgaccagctt
    12121 tcatctcgag cgggacgaga agccgtttct caaccggttc ctggccaggt ttcccacggt
    12181 caccgtcatc gaagtcgatg ccatcattgc tcaggttcag agcatcatcc tcagagtgac
    12241 ccaggccgtt gagctcgtgc tcgccctggt ggtggcgtcc ggatgcctgg tgctggtggc
    12301 cagcatccag gccagccgcg acggccgcat gagtgagctc gcgctggtcc gcacgctggg
    12361 gggcagccgc cgcctcatcg cggggtcgct ggtcgccgaa ttcgccgcgc tcggcctgtt
    12421 tgccggcacg gtcgccgtgg tcggggcgga agccacggtg gccatcctgc aaagccaggt
    12481 tttcgagctg ccggtgcgtc tgcatccctg gctgtggctc ctgggtccgg tgatcgggtc
    12541 cgtgctcatc ctcatcgtcg ggaccctggg cacgcgcggg ctgatctcta cgccccccgt
    12601 catggtgctg cgcggtctgg attgacccgt cgtgagttgt cagcgccgat ccgtgctgct
    12661 aaaaatccgt tcccacgcca tcccaaggcc ctgatgaagg cagacccaat ggaccccgcc
    12721 gcgaggtcgg agcgcgcaat gcgcaagcac ggctacgaca tgaacaagct gcacagaaag
    12781 ctcaggcgca aagttggcca ggccatcgcc gattacggaa tgatcgagga aggcgatcgg
    12841 gtgatggtct gcctgtcggg aggcaaggat tcctacacgc tgctggacat tctgctcaat
    12901 ctccagagaa gcgcgccggt cgccttcgag atcgtcgccg ttaacctgga tcagaagcag
    12961 cccgggtatc cggcggacgt gctgcccggg tacctggaag ggctggacgt ttcctatcac
    13021 atcctggaac aggacacgta ttccatcgtc aaggcggtca ctccggcggg caagacgacc
    13081 tgcccggttt gctcgcggct gcggcgcggc attctctaca acttcgctgc gagcatcggc
    13141 gcgagcaaga tcgcgctggg acaccacctt gatgacgtgg tggaaacgct gtttctcaac
    13201 atgttccatg gggcgcggct gaaggccatg ccgccgaagc tgaaaagcga cgacggacgc
    13261 aacgtggtga tacgaccgct ctactactgc cgggagcgcg acattgcccg ctgggccgag
    13321 caccgagagt atcccatcat cccctgcaac ctctgcggtt cccaggagaa tcttcagcgg
    13381 caggtgatca agtcgatgct cgcccgctgg gacgccgagc agcccggacg cgtggagaat
    13441 attgcccgca gcctcagtgc cgtggtgccg tcccatctcg gcgaccgcgg ccttttcgat
    13501 ttcgccggcc tttcctcgga cgatggcgtc gagaagcttc agctgctcga tctcacccct
    13561 cgttgcgtga acgcgatgcc gcctcggtga aggggtggtt ggccagggtc tctgccgatt
    13621 ccagcacctc gttcatggcc acctcgaaac tctgaaccag cccgagcagg ttgctctcgt
    13681 cgcccttggc cagagcgagc tcgagggtct ttgaagcctg cttgaggcgg tcggccccga
    13741 agctgccggc cacgccgtgc aggttgtggg cgagccgctc ggcctcctcg agatggttgt
    13801 cggtcacgtg ggcacgcatc tttgccccgg cgtcgccgta gtacctgccg aaatccccca
    13861 tgagtttcag catgaggcgg acgttgccgt tgtgtcccct gatggccgtg tccaggtcga
    13921 ttccaggcag ctgaatctca ggaagctcgc ccggcgtcgt tgacgcttcg tgctcggcag
    13981 gcgcggacgc cggagcatcg acgctagcgg cgtccgccgg ctgctcgggc agcacccggg
    14041 acagctcggc cagcagcagg tcgaagtcga tgggcttcga cacgtaggcg ttgcagcccg
    14101 cctccagcgc cgcttgctgg ctggcttcga tggcgtccgc ggagaccgcg atgatgggcg
    14161 tcgtgatctg ctgctcgcgc aggatccggc atgcctggag cccatccagt tcgggcatgt
    14221 gtatgtccat gagaatcgcg tcgtatgctt tgctggtcgc gcgctccacc gcctcgcgcc
    14281 cggtctgggc aatgtccacc cgtgccccgg tgcgctgcag gaactccagg gacagttgct
    14341 ggttgatggg gttgtcctcc gcgaccagga actctctgcg atttagcgcg cgcaggttgc
    14401 gccgccgctg acgcacgtct gcgggcatca ggtcctgagc gtcccgaagg ccgaacacgg
    14461 cggtgaaggt gaagcagctt ccctggtcgg gcaccgaggc cacggacatc tcgcccccca
    14521 tcagcgacac cagccgtttg cagatcgtca gccccagccc agtgcccccg tagcgccggg
    14581 tggtggaggt ctccgcctgc tcgaacgact cgaagagtcg tgcctggtgc tcgggtttga
    14641 tcccgatccc ggtgtctctg acggcgaact cgacgcgcag ctcgccggcc tcgcagggac
    14701 agacctgggc cgtgaccgag atgctcccct cctcggtgaa cttcagcgcg ttgctgacca
    14761 gattgaccag cacctgctga agcctcaggg ggtcgccgat gatcacgccg tcgccgggaa
    14821 actcggcgtg cgccgcggtg ttgtcgtcga agttgagctc gagattgctt ttgcgaacct
    14881 cggtccggaa cagacgttcc acctctttga gcgtgtcggc cagcttgaag ggtcggtgtt
    14941 cgagggtgag cttgcccgct tctattttcg agaagtccag aacgtcgttg accagcgaga
    15001 gcaggttctc cgatgcggcc cgtatggagg acaggtactc gtgctgcttg gggtcgagcc
    15061 cggtctccag cgccagcttg gagaagccga tgatggcatt catcggcgtc cggatctcgt
    15121 gactcatgtt ggccaggaac tcgcttttcg cctgggttgc ggcctcggcg atctgccggt
    15181 gcttgtcggc ctgctcccgc ttcatgcgct cggagatgtc gacgagagag ccgtcgatgt
    15241 aggcgccctg ctcggggtcg tggctcaggc gcgccgttag cgccatccag aatgtgcggc
    15301 cgttgcgcgt gaccccctgg gcctcgaaga cgttgagcag gccgtgctct tcgaggttgg
    15361 tgaggaagtg gctggcgtct tcccggctca ggaagacgcg gtcgatgaga tcacggtact
    15421 cggccagcag ctgcttgacg tcgtcgaagc cgagaattct tgccagcgtc gggttggcgc
    15481 tcagcagcgc gccgtcgggg tcgatccgga agagcccttc gatggcgttg tcgtacagcg
    15541 ctcggtactg ctcctgagtg tcgtgcaggt cggcgtacag ccgcgcgttc tcgatcgaga
    15601 tcgccgcctg agaggcgagc agggcgagga cttccacccg ctgggcagtg aaaacgccgg
    15661 tgagccagcg gtgctcgacg tagaggattc cggtgatctc gttgcggtgc acgatgggca
    15721 cgcagaggac ggacagcggc tgtttgcgaa cgatgtatgg atcctgggta aagacgtcct
    15781 ccgcggtggc gtcgccaagc accagcgcct gcttggtgcg cgccacgaag tgaacgatgc
    15841 tctcgggcac gtcgtcggag gcctccagcg gggtcgccgg gctctcgcgg cgggtcgggc
    15901 cgccgtccac cgaggcgatg gcttcgacga acagccttcc gtcgtgggcg agcagcatgc
    15961 aggctttctg ggcgccggca tgctccagcg ccaggcgcag cagcttcgtc aggacgcggt
    16021 cgaggacgat ctcgctggag atggtttgag ccgcgcgcag caccgtggtg gtgtccagga
    16081 cgttcgcgtt gatctcgctt ccatccaggc tcgaccggat ggacatggag ctgaagtcgc
    16141 gcacggtgag gtcggtcagg tctccgatca cggtcgcagg atgcagcgca tcgtcgagca
    16201 tgctcgggaa ttcccgctcg aggtgactgg ctttcgcatt tgcgccccag cgcaggtagg
    16261 cctgatgagc gttgcgcgcg aagagcttgg cgaagtcggc ccgaccgttg gactcgcagg
    16321 ctctggccgc cagctcgtag gccagcgcct cgtcgttggc aaggccgagc cgacgcgctt
    16381 tgtcggcggc gacctcgtag cactcgatgg cctgggtggt ggcgccgcgc tgccagttca
    16441 gctcggcctg cagcaggatg accttcggct ccgcgtggtc ggcgccgtcc tggtgccact
    16501 gtttcagctc acgcagcgac gcgcgtgccc ttcgtaggcc agccgtgttg cccgagcgca
    16561 ggttcaccag agcgtcggcg aatttccaga cgatgagcag aggcgacccc gccagcgcgg
    16621 ggccgaactg ctcggcgagc tcgagaatgt tggccgcccc cctgaaatcg tcgaagagca
    16681 cggcgtagta gaggcgcagc acgtacacgt agccgtgggc gaccgcgtcc tcgctgttgg
    16741 aaatgctcag ctccctgaag tggtccgact cgccgtcggc ttccgcatga cccagcaggc
    16801 tgccgatgat ctgctgaacg aacagcgaga tgttgacgcc cgtgacgtgc tgaagaccgg
    16861 aaagtctgga aatctgctcg ttcagctctc gccgtaggga cgccagatcg agcccgcgaa
    16921 gcaggccgtt ggttgcgtag aaggccgtgg ccgtggcagc aaactcgtag tcctggcagg
    16981 ccatgctggc gtcgatgttg cgcgaaagcc cgccaagcgt gtcgtccagc ccccccgacc
    17041 atgggtccac caggccgttc agcagggttt ccgcgcggat ggagaaggcg tcccctgaga
    17101 actggccggc aagggctcgc gcctccgcgg cgtagcgctg cgcgatcccg atccggtggt
    17161 cggcgacggc gctgacggca cgcgcggcga acgcaaaggc gatctcggcg ctgtagccgt
    17221 gccgtgccgc ctcgctgagc atgtcgtctg taagggcgtc caggccctcg ctgcccaggt
    17281 ggtatccggc gtgcaacagg tagccgagca accggaaggt gtgctgcagg cgaatgtcct
    17341 cgagcaccgg ggccggtttg ggcgcggcgt ccggcgcgcg cttcctgccg agcctgcgga
    17401 aagcctggcc gatggggttg atgccggttg cgggtcgcac gtaccccagg ctctcgaggg
    17461 cgtcgaacgc ctgctcgcgg gctgcgccca ggtgattctg gaccaccgcc gcgcgaaccc
    17521 tgatctcgtc cagggcgctg gtattgtgcg gaatgctggc gatgacccgg tcgagctgct
    17581 cgaagtcgct gcagtagaag gcggcctcgg cagcgctctg gcacagctcg atgaagatgg
    17641 gctcgtcggg cagctcgctc aagagcgaca gcccgctgcg gcagaacttg taggcgggct
    17701 ggaaggcgcc ctggccgata ctctcctttg cggcgagaag gttgtagtgc gcgatttcca
    17761 ggcgtttgtc cttgtcgagc ccaatgggat ccgcggcggc gttgaggtga tgggcgatct
    17821 gcaggagcgt cctgcccgcc ggcctgtggc cgcgcttgca ctcatcggcg atggcgaagt
    17881 gaatcgtcga cttggcttcc tgggccatct ccgcgtacac cagcgacctc acccttggat
    17941 gggcgaaccg gtagagcagc tggtgctgat ctcccggcgc ttccttgctg cccgtcgcgg
    18001 tggtgagcag gcctttggtc agcgccggcc gcagccgccc ggcga
//
LOCUS       DBNHLM010000370         5841 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_237876, whole genome shotgun sequence.
ACCESSION   DBNHLM010000370 DBNHLM010000000
VERSION     DBNHLM010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_237876"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="AC16QF_17185"
     CDS             complement(<1..362)
                     /locus_tag="AC16QF_17185"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6450346.1"
                     /translation="MMRIGISVSSSYQVDDPREGARNMIERARAARDAGLDSLFVGDH
                     HVTPFTYYQNTPILGRMLAEWSEMPFGALYLLPLWHPVVLAEQIATLASIAPGRFIMQ
                     CGLGDDRQGKAMGVDMSRR"
     gene            complement(376..936)
                     /locus_tag="AC16QF_17190"
     CDS             complement(376..936)
                     /locus_tag="AC16QF_17190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6450347.1"
                     /translation="MADETDTQATNYDDVRQYRLDPEREQELVNTAGECVFCWANKAG
                     HPIGVITAYVPVKGKIWMTATRERVRIKAIARDGRSSVVISSKGTPMGGGKTVTYKGH
                     TVIHDDQDTKMWMYNLLAQGMAASKDEGNEFTKGLDPEMFVRFLDTPERVVLEFTPEM
                     SIPFDGDKMAMGTAAAYAAFAKENQS"
     gene            1053..2411
                     /locus_tag="AC16QF_17195"
     CDS             1053..2411
                     /locus_tag="AC16QF_17195"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6450348.1"
                     /translation="MKPVRNKPSYREIIQGDATPVADVLALTANPQQSTEDIPFEHYI
                     SDEFFEREMRKMWPKVWQIACREEHIPEVGDYFVYDIGRYSILVVRSAEDQIKAYHNS
                     CLHRGTKLKPSGTAGFSEKLQCPFHGWTYQLDGTLEEVPCAWEFPHLDYEANRLPEVL
                     AESWNGFVFINMDQDAQPLLQYLEVLPEHFASWRFDDWYVASHVRKELHCNWKVAMEG
                     FMEAYHTPVVHPEMCQVVGDWNMQHDIFGDHVSRDLCPMGVSSPSLDEPLSEFELLRR
                     ARPGNPLTSETGSAELPDGKTARMVMAEETRRSFRDQFGMDLSDLSDAEVIDSLKYNV
                     FPNLMFSGGAAIHGLSIFRPLGYDTNKCTIDRLTFLPVPKDGRRPPPAEVITIAEGES
                     YATADTLNEFTAHVLDQDTSIMRLQQEGMHASAKGAETLSTYQESRVRWLHETLEKYL
                     SR"
     gene            2444..3670
                     /locus_tag="AC16QF_17200"
     CDS             2444..3670
                     /locus_tag="AC16QF_17200"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6450349.1"
                     /translation="MEKSEFFADARRDLTGPIHGIRVLEITTSWAGPMAGCMLADLGA
                     EVIKVEHPDGEIGRVLGPHLPGGAHLSLLYETANRNKRNLSVDMRKEVGRDLLIDLGR
                     TCDLVVENFKPGTLHGWGLGYEHFCAAKPDIVYVSISGFGQFGPHQARAAYDPLVQAQ
                     SGWMSLNGEIGGGPVKAPTFLGDDLAGMQAALAALAALRHRDATGEGQHVDVALMDAI
                     LGASNMFPSMARLGIEAPRTANQFPQVAPFNVYACRGGSVFLGMSLNSHWEIFANAVG
                     RPELVEDPRYATTELRTQNRESVDELVSRWCSEQDQMRVVELLGGAGLPVAPVNDYRA
                     ATDDPNIEARDMLTDIELSDGSRAPLLGPVPKFSRTPIGVRSGAPTIGQDNASILDEL
                     GIDDARRRALREAGVI"
     gene            3777..>5841
                     /locus_tag="AC16QF_17205"
     CDS             3777..>5841
                     /locus_tag="AC16QF_17205"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6450350.1"
                     /translation="MRTARFSVCMGARMNEGDRAGGSTARGAGVAFGLVLALLGSQAS
                     ASAIEEIVVSARAVEQSVRDVPVAITAFNEEVMDNMALTSFDDIAAYTPSVDILRISS
                     GNGTSVSIRGISSSAGSLGIEQSVAIVIDGVYYPQGRSINEGLFDTSQVAILKGPQAL
                     YFGKNATAGVISVMTNDPGDELEILGKIGYETELKTTRAEGVLSFPINDRWGVRLALQ
                     GTDMSDGWLENTAQDDVYVTFEGSQDNPAPKDAENPGEESFYGRVTLKGELSDAVTLR
                     LKGSYSDFEYNTMTLGEQYECSALNGVPHITLNGDPDPVTGWQRPTPLGLGECEANRA
                     RGLNPIPPAVAATSKDLSRFGGELGEDYESYSFTADLELDLENVFIKTFLNYHDQTVG
                     WVIDADNNEKTNIFASENNQFQNFSFETRAATRFDGPVNAVLGFYYQSTDRKFRQEVI
                     FAGAEDSTADPTNRFIAYDKISETDGETVSGYGELIWDVSEQWQVTAGARYIWENKDS
                     FFTQPYVNFAFSGLFVAGTLEDDSSFDDFIPEVTVRYQPNDDVTVYAAYKQGFKSGGF
                     DNGAIYSTLNPDPLADITFEPETVEGFEAGIKAALFDGTVNVELDAYYYEYEELQLNF
                     FNSNVFAYRTLNAGAAETTGSELQVQWAPPGVDGLTLLASVAYNDASYKDFIAPCSGG
                     QSFSGG"
BASE COUNT         1054 a         1845 c         1887 g         1055 t
ORIGIN      
        1 cgtcgggaca tgtccacccc catggccttg ccctgccggt cgtcgcccag cccgcactgc
       61 atgatgaagc gccccggcgc gatgctcgcc agggtggcga tctgctctgc cagcacgacg
      121 gggtgccaca ggggcagcag gtagagcgcg ccgaacggca tctcgctcca ctcggccagc
      181 atgcgtccca ggatcggcgt gttctgatag taggtgaacg gcgtgacgtg gtggtcgccg
      241 acgaacaggg agtcgagccc cgcgtccctg gcggcacggg cgcgctcgat catgttgcgc
      301 gcgccctcgc gggggtcatc aacctggtag ctggaagaga ccgatatgcc gatgcgcatc
      361 atgccgccct cgccgtcagg actggttttc cttggcaaac gccgcgtacg ccgccgccgt
      421 gcccatggcc atcttgtcgc cgtcgaaggg aatggacatc tccggggtga actccagcac
      481 gacgcgttcg ggagtatcca ggaagcgcac gaacatctcc gggtccagcc ccttggtgaa
      541 ctcgttgccc tcgtcttttg acgctgccat gccctgggcc aagaggttgt acatccacat
      601 cttggtatcc tggtcgtcgt ggatgaccgt gtggcctttg taggtaaccg tcttgccgcc
      661 acccatgggg gtgcccttgc tcgagatcac caccgatgac cggccgtctc tggcgatggc
      721 tttgatgcgc acgcgctcgc gggtggccgt catccatatc ttgcctttca cgggcacgta
      781 ggccgtgatc acgccgatgg ggtgacccgc tttgttcgcc cagcagaaga cgcactcgcc
      841 ggcggtgttg accagctcct gctcgcgttc cgggtccagc cgatactgcc ggacgtcgtc
      901 gtagttggtt gcctgggtat cggtttcatc ggccatcgtg gtgctccctt tcaacgtgcc
      961 ttcgccccgg tgtgttcgac gcttgatgcg tggttggccg gcaagtataa cggtttgcac
     1021 atcggctcga tgggccgtat ggggaggaca tcatgaagcc tgtgcgtaac aagccaagtt
     1081 accgggagat cattcaaggc gatgccacgc ctgttgcgga cgttctcgcg ctcacggcga
     1141 acccgcagca gagcacagaa gacattccgt tcgagcatta catctcagac gagttcttcg
     1201 aacgcgagat gcgcaagatg tggcccaaag tctggcagat cgcctgccgc gaggagcaca
     1261 tccccgaggt gggcgattac ttcgtctacg acatcggccg ctactcgatc ctggtggtgc
     1321 gcagcgcgga agatcagatc aaggcgtacc acaactcctg cctgcatcgc ggcaccaagc
     1381 tcaagccctc gggcactgct ggattctcgg agaagcttca gtgcccgttt cacggctgga
     1441 cctatcagct cgacgggact ctcgaggagg tgccctgcgc ctgggaattt cctcatctgg
     1501 attacgaggc caaccggttg ccggaggtgc tggctgagtc ctggaacggc ttcgtgttca
     1561 tcaacatgga ccaggacgcg cagccattgc ttcagtatct cgaggtgctg cccgagcatt
     1621 tcgcgagctg gcgcttcgat gactggtacg tggcgagcca cgtccgaaag gagcttcact
     1681 gcaactggaa ggtcgccatg gaaggcttca tggaggccta ccacacgccc gtggtgcacc
     1741 ccgagatgtg ccaggtggtc ggcgactgga acatgcagca cgacatattc ggagaccacg
     1801 tcagccgcga tctgtgtccc atgggcgtct cgagcccgtc tctcgacgag ccgttgagcg
     1861 agttcgagct gctaaggcgc gcgcggccgg gcaacccgct gacgtcggag accggatcag
     1921 ccgagcttcc ggacggcaag acggcccgga tggtgatggc cgaggagacc cgacgcagtt
     1981 tcagggatca gttcggtatg gacctctccg atctcagcga tgcggaggtg atcgattctc
     2041 tgaaatacaa cgtctttccc aatctgatgt tctccggcgg tgcggccatc cacgggctca
     2101 gcatatttcg ccccctcggc tacgacacca acaagtgcac catcgaccgc ttgacgtttc
     2161 tgcccgtccc gaaggacggt cggcggccgc cgccggcgga ggtgatcacc attgccgagg
     2221 gcgagtcata tgccacggcg gacaccctga acgagttcac cgcccacgtc ctcgaccagg
     2281 acaccagcat catgcggctg cagcaggagg gcatgcacgc ttcggccaag ggggcggaga
     2341 cgctctcgac ctatcaggaa tcccgcgttc gctggctcca cgagacgctg gagaagtatc
     2401 tttcccggta gcggcgcagc cgcagcagcg caagggggct agtttggaga agtcggagtt
     2461 ctttgcggac gctcgccgcg acctgaccgg tcccatccac ggtatccgcg tgctcgagat
     2521 caccacatcc tgggccgggc cgatggccgg ctgcatgctc gccgatctgg gtgccgaggt
     2581 catcaaggtg gagcacccgg acggcgagat cggccgggtt ctgggcccgc atctgccggg
     2641 aggcgctcac ctttctcttc tttacgagac cgccaaccgc aacaaacgca acctgagcgt
     2701 tgacatgcgc aaggaggttg gtcgcgacct gctcatcgac ctcggccgga cgtgcgatct
     2761 ggtggtggag aacttcaagc ccggaaccct gcacggttgg ggcctgggtt acgagcactt
     2821 ctgcgcggca aagcccgaca tcgtctacgt ttccatcagc gggttcggtc agttcgggcc
     2881 acaccaggca cgggccgcct acgacccgct ggtgcaggcg caatcggggt ggatgtccct
     2941 gaacggagag atcggcggcg gcccggtcaa ggcgcccacc ttcctgggcg atgacctggc
     3001 cgggatgcag gcagcgctgg ccgcgctggc tgcgcttcga catcgggacg ccaccggtga
     3061 gggccagcac gtggacgttg cgctgatgga cgccattctc ggcgcctcca acatgttccc
     3121 gtccatggcg cggctcggca tcgaagcgcc ccgcacggcg aaccagtttc cccaggtagc
     3181 gcccttcaac gtctacgcct gccgcggcgg ctcggtcttt ctcggcatgt cgctgaactc
     3241 ccactgggag atctttgcca atgccgtggg gcgcccggag ctggtcgagg atccccgtta
     3301 cgcgaccacc gagctgcgca cgcagaatcg ggagagcgtc gatgagcttg tttcgcggtg
     3361 gtgcagcgag caggaccaga tgcgcgtggt ggagctgctc ggcggcgcgg gcctgcccgt
     3421 ggcgccggtc aacgactacc gcgccgcgac ggacgatccc aacatcgaag cccgcgacat
     3481 gctgaccgac atcgagctct cggatggcag ccgggctccg ttgctcgggc cggtgccgaa
     3541 gttctcccgt acgccaatcg gcgtgcgctc gggcgcgccg accatcgggc aggacaacgc
     3601 ctcaattctc gatgagctcg gcatcgacga cgcgcggcgc cgggcgttgc gggaggccgg
     3661 ggtgatatga acggggccgt cgctgctgcg tgagccgaag cgtcggggtg gttgcctgca
     3721 ggtccggaag gcgcacataa tcaaaagagt tgaatccatc cctggggagg gtagatatga
     3781 gaacggcgag attctctgtg tgcatgggtg cgcgcatgaa cgagggcgat agagctggcg
     3841 ggtcgaccgc acgtggggcc ggcgtcgctt tcggcctggt cctggcgctg ctcggtagcc
     3901 aggcgtctgc cagcgccatc gaggagatcg tggtttccgc ccgggccgtg gagcagtccg
     3961 ttcgggacgt gccggtggcc attacggcgt tcaacgagga ggtcatggac aacatggcgc
     4021 tgaccagctt cgacgacatc gcggcatata ccccatcggt ggacatcctt cgcatatcga
     4081 gtggcaacgg aacgtccgtc agcatccggg ggatatcctc cagtgccggg agcctgggca
     4141 tcgagcagtc cgttgcgatc gtcatcgacg gggtctacta cccccagggc cgctccatca
     4201 acgaaggcct cttcgacacc agccaggtgg cgattctcaa agggccccag gcgctgtatt
     4261 tcggcaagaa cgccactgcc ggggtgatct cggtgatgac caacgatccc ggcgacgagc
     4321 tggagatcct gggcaagatc ggctacgaga cggagctcaa gaccacccgc gccgaggggg
     4381 tcctgtcttt ccccatcaac gaccgatggg gtgtgcgtct ggcgctgcag ggcaccgaca
     4441 tgtccgacgg ctggctcgag aacacggcgc aggacgacgt ttacgttacg ttcgagggca
     4501 gtcaggataa tcctgcgccc aaagacgcgg agaaccccgg ggaagagtcg ttctacggcc
     4561 gcgtgaccct caagggcgag ctcagcgacg cggtgaccct gaggctcaag gggtcgtact
     4621 cggatttcga gtacaacacc atgaccctgg gggaacagta cgagtgctct gcgctcaatg
     4681 gcgttcccca catcaccctg aatggcgacc ctgacccggt caccggctgg cagcggccga
     4741 cgccgctggg cctgggagag tgcgaggcga atcgggcgcg tgggctgaat cccataccgc
     4801 cggcagtcgc ggcgacgtcc aaggacctca gccgcttcgg cggggagctg ggcgaggact
     4861 acgagtccta cagcttcacg gctgatctgg agctcgatct ggagaatgtg ttcatcaaga
     4921 cgttcctcaa ctatcacgac cagaccgtgg gctgggtcat cgacgcggac aacaatgaga
     4981 agaccaacat cttcgccagc gagaacaacc agttccagaa cttctccttc gagacccgcg
     5041 cggccacccg gtttgacggc cccgtcaacg ccgtgctggg cttctactac cagagcacgg
     5101 accggaagtt ccgccaggag gtgatcttcg ccggcgcgga ggactccacg gcggatccga
     5161 ccaatcgctt catcgcctac gacaagatca gcgagaccga cggcgagacg gtctccggct
     5221 acggggagct gatctgggac gtttccgagc agtggcaggt cacggcaggc gcgcgctaca
     5281 tctgggagaa caaggactcg tttttcaccc agccctacgt gaacttcgct ttctcagggc
     5341 tcttcgtggc gggcactttg gaagacgaca gctcctttga cgacttcatc cctgaggtga
     5401 ccgtccgtta tcagcccaac gacgacgtca ccgtttacgc ggcctacaag cagggcttca
     5461 agtccggtgg cttcgacaac ggcgccatct acagcaccct gaacccggac cccctggccg
     5521 acatcacctt cgagccggag acggtggagg gcttcgaagc cggcatcaag gcagccctgt
     5581 tcgacggtac cgtgaacgtg gagctcgacg cctactacta cgagtacgag gagctgcagc
     5641 tgaacttctt caactccaac gtgtttgcct atcggacgtt gaacgccggc gcggcggaga
     5701 cgacgggcag cgagctgcag gttcaatggg cgccgccggg cgtcgatggc ctgacgctcc
     5761 tggcctcggt ggcctacaac gatgcgagct acaaggactt catcgccccg tgcagcggcg
     5821 ggcagtcctt cagcggcggc t
//
LOCUS       DBNHLM010000371        11166 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_835293, whole genome shotgun sequence.
ACCESSION   DBNHLM010000371 DBNHLM010000000
VERSION     DBNHLM010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11166)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11166)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11166
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_835293"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            366..809
                     /locus_tag="AC16QF_17210"
     CDS             366..809
                     /locus_tag="AC16QF_17210"
                     /inference="COORDINATES: protein motif:HMM:NF025691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HIR6450351.1"
                     /translation="MEEVMKQRLTSRNLWRRVLYMLLFVLAYGIAEAVVAVVVVVQFF
                     FILISGRANENLLVFGNNLSTYVRQILQFQTFNSEYLAYPFGDWPDEGLGSELWLEEG
                     TAEEAEAAPPVAGSEPPDEAEPDSGVAERGPIEGSADPEEPDPRG"
     gene            complement(826..1158)
                     /locus_tag="AC16QF_17215"
     CDS             complement(826..1158)
                     /locus_tag="AC16QF_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450352.1"
                     /translation="MNQRVVAGILALALCLHALPASARLYSYVNEEGDYVVSQKKPKN
                     VDEYAVLTDEGEFIRLVKRKRDDVPITHWRPWYMPNEPDPINPVEPGLEQRTPEVLIE
                     EVDGPEPN"
     gene            complement(1213..4575)
                     /locus_tag="AC16QF_17220"
     CDS             complement(1213..4575)
                     /locus_tag="AC16QF_17220"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6450353.1"
                     /translation="MNGPIAWFARNHIAANLLLAVMVLAGLLSLPIMPQKDFPDLDYK
                     LITVSVRYLGAAPEEVEDGVCIRIEEAIEGLQGIERIRSNAVEGRCTVNVELFENADD
                     NKVLGDVKNRVDAIETFPQEAEKPIITLVAAERTVVEIAVTGPSDERELKSIAQQVRD
                     DLAALPGITRVDVSGVRPYEISIEISEATLRRYGLSFDEVANAVRAASLDLPGGAIKT
                     ESGEVLLRTEGQAYRGSEFEDLVILTRGDGTRVFLRDLATVVDGFEDTDERVWFDNKP
                     AALVQVFRVGDQDILAVTRAVKSYLDDADERLPQGVDLTIWNDNSEFMRDRLDTLMNS
                     ARQGFMLVLLMLALFLRPRLAFWVSVGVPAAFLGAIFVANLAGLSIDGISTFGFILVL
                     GILVDDAVVIGERVHSLQSRAGESPPSETLISAAIEGTRQVSMPVIFGVLTTITAFLP
                     LTFGPGPTGQIQSVISVIVLCCLTFSLIESQLVLPAHLGHRRVQTAGGEVGLMMVPLV
                     AIVLMEFAWDLRSFFAIGIGTTAVLIAWSIQGGFERFAARVIALQKQFSDRLEHVIQF
                     RFRRLVSAATDARYVTAAGAVAILGITVGLVAGGHMPFTYFPSLPADRISAKLTMPLG
                     TPARVTEEIVTNIAVTAEVLQRELASEQPDQPPVRFILAAVGAHPVSSAGLGAVIAPP
                     AARTGGHMGEVTLQLTPSDERDLSTLEIANRWRDATGPIPDAVEFTFATSTFSVGSEI
                     DIQLAGPDLEELANVAAEIRSELAAYPGVIDITDSFRSGKRELKLDILPAGEALGLNL
                     GALARQVRQAFYGEEIQRIQRGRDDVRVMLRYTEAERRALGSLDEMRIRTRNGSEVPF
                     ATVASADLGRGFSAIKRTDGRRVVNVTADVDRNVITANEVLADFGSGPLRTILRDHPR
                     VSFSLEGTRREQEDSIASLLPMFLMALAVIYALLAVPLRSYTQPLIIMSVIPFAMAGA
                     VWGHMIMKSLGLLSNIVIMSVTGGIAACGVVVNSSLVLVHGVNARRREGMDIHSAVVE
                     AAVTRCRPILLTSLTTFAGLSPLLLNQSVQAQILIPMATSVAWGVVFATVVTLLVVPV
                     GYLILDDLGRKLSRGRTSVSPGSADAGASAGR"
     gene            complement(4572..5735)
                     /locus_tag="AC16QF_17225"
     CDS             complement(4572..5735)
                     /locus_tag="AC16QF_17225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6450354.1"
                     /translation="MNRRLQAALVLLGGFAVAVLVFLSGPKMQPQAPSAIPPLVRVIE
                     AEPREVQMTSHTHGTVVPRTESDLVPEVDGRVTAVSPSLVSGGFFNQGDVLLEIEPVD
                     YEVALEHARAGLARAESDLENARKSLTRIQDLVRRGATSDSDRDDAANRERIAQATLR
                     EARARLSRAERDLDRTRLVAPYQGRVRNERVDVGQFVKRGNPVATIYAVDFAEVRLPI
                     QDEELAFLDLPLGYTGDESSIPIPVTLRARFAGVEHEWQGEVVRTEGELDPATRMVHV
                     VARVPEPYVSRDGRPPLSVGLFVDADIQGKTLSDVVILPRSALRGGDRVLVVDQDERL
                     RFRPVDVYRVAEEEVYIVGGLARGERVCISPLQSTSDGMLVRVADVEQRAAPS"
     gene            complement(5732..6406)
                     /locus_tag="AC16QF_17230"
     CDS             complement(5732..6406)
                     /locus_tag="AC16QF_17230"
                     /inference="COORDINATES: protein motif:HMM:NF037008.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6450355.1"
                     /translation="MAQASRPLRADSAATRESILEASVKLFGERGYRNTSLRAITDAA
                     DVNLAAANYHFGSKAQLLDAAFERCIAPINDERMRRLRQLQASSSSPSVEALVRAFVD
                     LEFALTDNEHLDQFLAHLFAEPKSVSVPLLEKTFAPVVQAYFAALHAALPDVPVEVLR
                     WRFHLMIGSMIQLARFRHPLNFFQADSPPAEGSLQAGIDQLVEFVVAGLTQPGTSGDG
                     ASGDGE"
     gene            6555..6929
                     /locus_tag="AC16QF_17235"
     CDS             6555..6929
                     /locus_tag="AC16QF_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6450356.1"
                     /translation="MLGYATIGVNDMDRAVAFYDALLAELGAKQLMGMDRIKMYGTSP
                     DAGMLAICIPYDEEPQSPGNGNMVAISAGSREAVDRLYAKAMELGAADEGEPGERNPV
                     FYGAYVRDLDGNKLCFYEMKLG"
     gene            6951..7490
                     /locus_tag="AC16QF_17240"
     CDS             6951..7490
                     /locus_tag="AC16QF_17240"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6450357.1"
                     /translation="MRSAQAAPATDGESERLHRRYRQGRVFPRAVVASVDIQAPAREV
                     WAVLVDFARYPEWNPFTPEVEAELAVGAPVRLRVSMPGRRELERVEQINRVEPERALC
                     WGMHMGHRALLCANRWQVLEPVRAATRYTTADRFSGLLVPLVMALYGGPMQLGFQQMA
                     DALKVRVEGTLPAGGAAGR"
     gene            7498..8679
                     /locus_tag="AC16QF_17245"
     CDS             7498..8679
                     /locus_tag="AC16QF_17245"
                     /inference="COORDINATES: protein motif:HMM:NF018452.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HIR6450358.1"
                     /translation="MRKLAVGLLGLFRRGALLWHKMRGRTLDDVAAERIVSGRSWEEF
                     CDTLKAAGAALTFPGAPRDPFNQAEGYRYLTRLTRAGLEAFVEYADAAAPVIHRVAHE
                     TVKLGSDNPDNYYQTARINGAWDYRITGRRNTIHYLSFGTQIGHYGQGGGMPPTGFID
                     DKDLEIDPDGSFTIVLSPRRQPGNWLPMTEETGTLIVRQTYLDRAAEEPADLSIERVD
                     GGGTPGQVTPALMDAGLKDASTLVAGASLLFAKWARDFQSHTNRLPMFDQEVSNRAGG
                     DPNITYYHSHWTLAPDEALVIESLPPACEHWNFQLNNYWMESLDDRYHTIHTNKHLAT
                     YRPDGSVRIVVAHQDPGVPNWISTVGHASGTMSFRWIRAEATPEPTTRVVRLADVASL
                     A"
     gene            8672..9916
                     /locus_tag="AC16QF_17250"
     CDS             8672..9916
                     /locus_tag="AC16QF_17250"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR6450359.1"
                     /translation="MPDFRPPRLPWPVRAFNRMGPALGATGLLPALDAEHMMAEARRR
                     AGLSDFGEDWFRAPLDALLGSINGEARLSALGRTIIRKRIVDALTTRLRARALLDAYP
                     EIESFDPGRVLVIAGLQRTGTTLLHRLLGADPRIRAVLSWEALNPVPLSGPAERERRI
                     RQALRAERGLKYIAPEFFAIHPVEHDMPEEDVLLLDVSFMSQSAEATMHVPTYARWLE
                     EHDHAPAYRYLRQLLSVLLWQRHAPTLLLKSPHHLEHLDTVLDVFPNATIIQTHRDPQ
                     QTVPSFASMVCHGASIFSDRVDVPRLTDHWLRKIRRVVERSLTARDRHGTARFVDVSY
                     YDLIGDPMAELARVYEAAGLAFDPQAQQGARETLRRQARDRFGRHRYRREDFGLTREL
                     IEGELGFYRRRFDIPFEEEAAS"
     gene            9913..10929
                     /locus_tag="AC16QF_17255"
     CDS             9913..10929
                     /locus_tag="AC16QF_17255"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6450360.1"
                     /translation="MNVKPTTDAPNAVGHRGVVSALVTALSDLVGRRDQLEPMLPDER
                     LDGRTYLVTGASSGLGKAAAVRLASAGAEVIMACRSGIPEAGDDVMRASGSGQVRMLE
                     VDLFDLESASAMCDRLRDDKQHLDGVVFNAGLMPLSARRGPQGFEEMFAVHFLSSRLI
                     VERLLADGVVAPSVDPATRPRLVFVSSEAHRSARPIDFEQFGAFVDYGIRGGMAEYGY
                     SKLHLCTYAVELARRLSSSDGPGVAVHALCPGPVASNIAREAPPWLKPVVGPIIRWLF
                     APPERAVEPVLLLAAGRAVEGRTGVYLHMMREKTFSSLAADPDNGRRLWEASGALIEP
                     FLRR"
     gene            complement(10919..>11166)
                     /locus_tag="AC16QF_17260"
     CDS             complement(10919..>11166)
                     /locus_tag="AC16QF_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanone monooxygenase"
                     /protein_id="HIR6450361.1"
                     /translation="HLYANGISRIETTQAAEKGWVRHVAEEGDATLFPLANSWYVGAN
                     IPGKTRVFMPYVGGMGKYRDHCDRSAKHGYEGFVLSA"
BASE COUNT         1907 a         3629 c         3709 g         1921 t
ORIGIN      
        1 tacccgatgg ctgaagttcc atcagatcga ctcgtccggc tatccccggg tgcacgacca
       61 cctcgggcgc atgggggaac gtcccgccgt tcgcgcggcg cttgaggccg aggccgccgc
      121 ggcttgagtc gccggaacgc ccccgcgccc gcctgtgggg tgtgcggctt cgcctcaatg
      181 tggtgaatct cgccatcttc gaggtgggcc tgtgagggca accatctgaa aatcatggga
      241 aaaaagaatt ggtaccaatc ttgcgacact gcctggcaac agagggcccc aagcggcccg
      301 ggcgaactcg cccctgtgtc cgtcgaaccc tcagccgtga tgtgttggca aatcaaggag
      361 gcgccatgga agaagtgatg aagcaacggc tcacgagccg aaacctctgg aggcgcgtcc
      421 tttacatgct gctcttcgtg cttgcctacg gcattgccga ggcggtcgtg gcggtggtgg
      481 tggtcgtgca gttcttcttc atcctgatct cgggtcgggc caacgagaac ctgctcgttt
      541 tcgggaacaa tctcagcacc tatgtccgcc agatcctgca gtttcagacc ttcaacagcg
      601 agtatctggc ttatccgttc ggggactggc cggacgaggg tctgggtagc gagctctggc
      661 tcgaggaggg cacggccgag gaggcggagg ccgcgccccc ggtggccggg tcagagcctc
      721 cggacgaagc ggagcccgac tccggcgtgg ccgaacgcgg tccgatcgag ggatcggctg
      781 atccggagga gcccgacccg agaggctgag cgcgatgggt ctcggctagt tgggctcggg
      841 cccgtcgacc tcctcgatga gcacctccgg ggtacgttgc tccagcccgg gctccacggg
      901 attgatggga tccggctcgt ttggcatgta ccacgggcgc cagtgggtga tggggacgtc
      961 gtcgcgcttg cgcttgacca gacgaatgaa ctcgccctca tcggtaagga cggcgtactc
     1021 gtccacgttc ttcggctttt tctgcgaaac cacgtagtcg ccctcctcgt tgacgtacga
     1081 gtagaggcgg gccgatgcgg gtagcgcgtg caggcagagt gcgagcgcga ggatgccggc
     1141 aacgacccgc tggttcatct ggtcgatccg ctggctgttt tgctggagcg gcgtcattgt
     1201 ctgcatatca cctcaccgcc cggcggaggc tccggcgtcg gcgctgcctg gcgaaacgga
     1261 cgtccggccc cggctgagct tcctgccgag atcatcgagt ataaggtaac cgacgggcac
     1321 caccagcagg gtgaccaccg tggcgaagac gacgccccag gctacggagg tggccatggg
     1381 gatgaggatc tgcgcctgca cgctctggtt gagcagcagg ggtgacaggc cggcaaacgt
     1441 agtgagtgac gtcagcagga ttggccggca gcgcgttacc gctgcctcga ccaccgcgct
     1501 gtggatgtcc atgccctctc gacgccgcgc gtttacaccg tgcaccagca cgaggctcga
     1561 gttgacgacg accccgcagg ctgcgatgcc acccgtcacc gacatgatca cgatgttgga
     1621 cagcagccca agcgacttca tgatcatgtg cccccacacg gccccggcca tggcaaaggg
     1681 gatcaccgac atgatgatca gcggctgcgt gtacgagcgc agcggcacgg ccagcagagc
     1741 gtaaattacc gccagcgcca tcaggaacat ggggagcagg ctggcgatac tgtcctcctg
     1801 ctcccggcgt gtgccttcca ggctgaagct gaccctgggg tggtcgcgca gaatggtcct
     1861 cagcggtcct gaaccgaaat cggccagcac ctcgttcgcc gtgatcacgt ttcgatccac
     1921 gtcggcggtc acgttcacca cgcggcgacc gtcggttcgc ttgatggcgg agaagccgcg
     1981 gccgagatcg gcgctcgcaa ccgtggcgaa gggaacctcg gagccgttgc gggtgcggat
     2041 gcgcatctcg tcaagggacc cgagagcgcg gcgctccgcc tcggtgtagc gcagcatcac
     2101 ccggacgtcg tcgcggccgc gctgaatccg ctgaatttct tcgccgtaga acgcctgtcg
     2161 gacctggcga gccagggcgc ccaggttcag cccgagcgct tctcccgccg ggaggatatc
     2221 gagcttcagc tcccgttttc cggagcggaa agagtcggtg atatcgatga cgcccgggta
     2281 ggcggccagc tcgctgcgaa tctcggccgc cacgttcgcc aactcttcca gatcgggccc
     2341 ggcgagttgg atgtcgatct cggagcctac gctgaaagtg gaggtcgcga aggtaaactc
     2401 cacggcatcc gggatggggc cggttgcgtc ccgccatcgg ttcgcgatct ccagggtgct
     2461 gaggtcgcgt tcgtcgctgg gcgtaagctg cagcgttacc tcccccatgt gccctcccgt
     2521 cctggcggcc ggaggcgcga tgacggcgcc caggccggcg gagctcacgg ggtgcgcgcc
     2581 cacggccgcc aggatgaagc gcacgggcgg ctgatcgggc tgttcgctgg cgagctctcg
     2641 ctgcagcacc tccgccgtga cggcgatgtt ggtgacgatt tcctcggtga cccgggccgg
     2701 cgttcccaga ggcatcgtga gcttcgcgct gatccggtcc gccggcagcg agggaaaata
     2761 agtgaacggc atgtgtccgc cggcgaccag gccaaccgtt atcccgagaa tggccaccgc
     2821 gccggccgcg gtgacgtagc gggcgtccgt ggccgcgctg accaggcgcc ggaagcggaa
     2881 ctggatgacg tgctcgaggc ggtcggaaaa ttgcttctgc agggcgatca cccgagcggc
     2941 aaagcgctcg aagcctcctt ggatgctcca ggcgatcagc accgccgtcg tgccaatgcc
     3001 gatggcaaaa aagctgcgca ggtcccaggc gaactccatc agcacgatgg ccaccagcgg
     3061 aaccatcatc aggcccacct cgcccccggc ggtctgcacg cggcggtgac ccagatgcgc
     3121 gggcagtacc agctgggatt cgatcagcga gaacgtcagg caacaaagca cgatcacgct
     3181 gatcaccgac tggatctgcc ctgtgggccc cggcccgaag gtgagcggta agaacgccgt
     3241 gatcgtggtc agcaccccga agatgacggg catggatacc tgtcgtgtgc cctcgatggc
     3301 cgcgctgatg agcgtttcac tcgggggtga ctcacccgcc cgggactgca gagagtgcac
     3361 gcgctcgccg atcaccaccg cgtcatccac cagaatgccc agcacgagta tgaagccgaa
     3421 ggtggaaatg ccgtcgatgg acaaaccggc cagattggcg acgaagattg cgcccagaaa
     3481 ggccgcgggt acgcccacgc tgacccagaa agccaggcgt ggacgtagga acaaggcgag
     3541 catcaggagc accagcatga atccctgccg cgcgctgttc atcagggtat ccagacgatc
     3601 tcgcatgaac tcggaattgt cgttccagat cgtcagatcc acgccctgtg gcagacgttc
     3661 gtcggcatca tccaggtagg atttcacggc tctggtcact gccagaatgt cctggtcgcc
     3721 cactcggaaa acctgcacga gcgcggcggg cttgttgtcg aaccagaccc gctcgtcggt
     3781 gtcctcgaag ccgtcgacca ctgtggccag gtcccgcagg aacacacggg taccatcccc
     3841 gcgcgtgagt atcaccaggt cctcgaactc tgagccccgg tatgcctgac cctcggtgcg
     3901 cagcaggact tcgccgcttt cggtcttgat ggcgccgcct ggaaggtcca gagaggcggc
     3961 gcgcacggcg ttggcgacct cgtcgaagct caaaccgtag cggcgcaggg tcgcctcgga
     4021 aatttcgatg gagatctcgt agggccgcac gccagagacg tccacccggg tgattcccgg
     4081 aagagctgcc agatcgtcgc gcacctgttg agcaatggac ttgagctccc gctcgtcgct
     4141 cgggccggtc acggcaatct cgaccacggt tctctctgcc gcgaccagcg tgatgatggg
     4201 tttctcagcc tcctggggga acgtctcgat ggcgtcgacc cgattcttga cgtcgccgag
     4261 aaccttgttg tcatcggcgt tctcgaacag ctccacgttc accgtgcagc ggccctccac
     4321 ggcattggat cggatgcgct cgatcccctg taggccctcg atggcttcct cgatgcggat
     4381 gcacacgccg tcctcgacct cttcgggagc ggcacccagg tagcgcaccg acaccgtgat
     4441 cagtttgtag tccaggtccg ggaagtcctt ttgaggcatg atcggcaggg atagcaggcc
     4501 ggccagaacc atgaccgcaa gcagaaggtt ggctgcgatg tggttgcggg caaaccaggc
     4561 gatggggccg ttcatgaggg cgcggctcgc tgctcgacat cggcaacgcg aaccagcatg
     4621 ccatcgctgg tgctctgcag cggcgagatg cagacccgct cgccccgggc caggccgccg
     4681 acgatgtaga cctcctcctc tgccactcgg tagacgtcaa cgggcctgaa gcgcagcctc
     4741 tcgtcctgat ccacgacgag cacccgatcg cctcccctca gtgccgatcg gggcagaatg
     4801 accacgtcac tgagcgtttt gccttgaatg tccgcatcga cgaagagccc gaccgacaga
     4861 ggcggccgcc cgtcccggct gacgtagggc tcgggaactc ttgcgactac gtgcaccatg
     4921 cgggtcgccg ggtccagctc accttccgta cgcacgacct cgccctgcca ctcatgctcc
     4981 acgccggcga aacgggcacg cagggtcacc ggtatgggga tcgaagactc gtcgcccgta
     5041 tacccgagcg gcagatcgag aaacgcgagc tcctcgtctt gaatgggtag ccggacctcc
     5101 gcgaagtcca cggcgtagat cgtcgccacc gggttgcccc gcttgacgaa ctggccgacg
     5161 tccacccttt cgttgcgaac ccgcccctgg tagggcgcga ccaggcgggt cctgtccagg
     5221 tcgcgttccg ctctggagag ccgcgcccgc gcttcgcgca gcgtggcctg agcaatgcgt
     5281 tcccggtttg ccgcgtcgtc ccgatccgag tcgctggtcg ctccgcggcg gacgagatcc
     5341 tgaatgcggg tcagtgactt cctggcgttc tccagatcac tctctgccct ggccaaacct
     5401 gcccgggcat gctcgagggc cacctcgtag tcgacgggtt cgatctccag cagcacgtcg
     5461 ccctggttga agaaaccgcc ggagacgagg gacggcgaga ctgccgtgac acggccgtcc
     5521 acctccggca ccagatcgct ttccgtgcgc gggaccaccg tgccgtgggt gtggctcgtc
     5581 atctgcacct cacggggctc ggcctcgatg acccgtacga gcggaggaat ggcgcttggc
     5641 gcctggggct gcattttcgg cccgctcaga aacaccagca cggccacggc aaagccccct
     5701 aggagcacca gcgcggcctg aagccttcga ttcattcacc gtctccgctg gcaccgtctc
     5761 cgctggttcc cggctgggtg agccccgcaa cgacgaactc cacgagctga tcgatgcctg
     5821 cttgcaggga gccttctgcg gggggcgagt ctgcctgaaa gaagttcaat gggtgtctga
     5881 accgcgccag ctggatcatc gatccgatca tgaggtgaaa gcgccagcgc aatacctcca
     5941 cggggacgtc gggcagcgcg gcgtgcaggg cggcgaagta ggcctgcaca accggcgcga
     6001 aggtcttctc gagcagcggc acggagacgg atttcggttc cgcgaacaga tgggcgagga
     6061 actggtcgag gtgctcgttg tccgtgagcg cgaattccag atcgacgaag gcgcgaacga
     6121 gcgcttccac cgaaggcgag ctggacgacg cctgaagctg gcgcaggcgc cgcatgcgct
     6181 cgtcgttgat tggcgcgatg cagcgctcga acgctgcatc gagcagctga gcctttgacc
     6241 cgaagtgata attggcggcg gccaggttca cgtcggcggc gtcggtaatg gccctcagcg
     6301 acgtgttgcg gtagccgcgc tcgccgaaaa gcttcaccga ggcttcgaga atactttccc
     6361 gggtggctgc cgagtctgcg cgcaacggtc tggatgcctg agccacgcgg cattcctagt
     6421 tcaaacggtc gtttaaatta gcacgcggtg ctcgggactg tcacgcgctg attgcgaact
     6481 cggctggcac gcggggcgcg gcggcgcgac aatgccgctc gctatctctc aacgagccaa
     6541 aaggagggcc tctcatgctg ggctacgcaa ccattggggt gaatgacatg gatcgggcgg
     6601 tcgccttcta cgatgcgctg ctcgcggagc tgggtgcaaa gcagctcatg ggaatggacc
     6661 gcatcaagat gtacggaacc agtccggatg ccggcatgct ggccatctgc atcccttacg
     6721 atgaagaacc tcaaagcccc ggcaatggca acatggtggc catatccgcc ggatcccggg
     6781 aggccgtgga tcggctctac gccaaggcga tggagctcgg tgctgccgac gagggagaac
     6841 ccggcgagcg caatcccgtc ttctatggcg cctacgtcag ggatctggac ggcaacaagc
     6901 tctgcttcta cgagatgaag ctggggtagc cgccaggcgg cgatccggtc atgcgatccg
     6961 ctcaggcggc gcccgcgacg gacggtgaaa gcgagcgact tcatcggcgg taccggcagg
     7021 ggcgcgtgtt tcctcgcgcc gtggttgcca gcgtggacat ccaggcgccg gcccgggagg
     7081 tctgggcagt gctggtggat tttgcccgct atccggaatg gaacccgttc acgcctgagg
     7141 tagaggcgga gctggctgtg ggcgcgccgg ttcgcctgcg ggtgagcatg ccgggtcggc
     7201 gcgagctaga gcgggtggag cagatcaatc gggtggaacc ggagcgcgcg ctctgctggg
     7261 gcatgcacat gggtcatcgg gccctgctgt gcgccaaccg ctggcaggtt ctcgagccgg
     7321 tgagggcggc cacgcgatac accaccgcgg accggttcag cggtctcctg gtgccgctgg
     7381 tcatggcgct ctacgggggc ccgatgcagc ttggcttcca gcagatggcc gacgcgctga
     7441 aggtgcgtgt agaaggcacg ctgcccgccg gcggagctgc cggccgatga ggagatcatg
     7501 agaaaactcg cggtggggtt gctcggcctg tttcgcaggg gtgcgctgct ctggcacaag
     7561 atgcgcgggc ggaccctgga cgacgtggct gccgagcgca tcgtcagcgg ccgttcctgg
     7621 gaggagttct gcgacaccct caaagccgct ggcgcggcgc tgacctttcc gggcgctccg
     7681 cgcgatccct tcaaccaggc cgaaggctac cgctacctca cccggctgac ccgggcgggg
     7741 ctcgaagctt tcgtggagta tgccgacgcc gcggcgccgg tcatccatcg cgtcgcccac
     7801 gagacggtca agctcggctc cgacaatccc gacaactact accagacggc gcgcatcaac
     7861 ggcgcctggg attaccgcat caccggcagg cgaaacacga tccactacct gagcttcggc
     7921 acccagatcg gccactacgg tcaggggggc ggcatgccgc ccacgggctt catcgacgac
     7981 aaagacctgg agatcgatcc ggacggctcg ttcaccatcg tgctcagccc tcgccggcag
     8041 cccggtaact ggttacccat gaccgaagag acgggaaccc tcatcgtccg tcagacctac
     8101 ctcgaccgtg cggccgagga gcccgccgat ctgtctatcg agcgggtgga cggcggcggc
     8161 acgccgggtc aggtgacccc ggcactgatg gacgcgggcc tcaaggatgc gagcacgctg
     8221 gtggcaggcg catcgctcct gttcgctaag tgggcgaggg atttccagtc ccacaccaac
     8281 cggttgccca tgttcgatca ggaggtctcc aaccgggcgg gcggggaccc caacatcact
     8341 tactatcaca gtcactggac gctcgccccc gacgaggcgc tggtcatcga gtccctgccg
     8401 ccggcctgcg agcactggaa cttccagctg aacaactact ggatggaaag cctcgacgac
     8461 cgctaccaca cgattcacac gaacaagcac ctggccacct accgcccgga cggctcggtg
     8521 cggatcgtgg tcgctcacca ggatccgggg gttccgaact ggatcagcac cgtgggtcac
     8581 gcttcgggaa ccatgagctt ccgctggatt cgggcggagg cgacgccgga gccgaccacc
     8641 cgggtcgtca ggctagccga cgtggcgagc cttgcctgac ttccgccctc cgcggctccc
     8701 gtggcccgtg cgcgccttca accggatggg ccccgcgctt ggtgcgacgg ggctcctgcc
     8761 agcattggat gccgagcaca tgatggcgga agcgcgtcgg cgagcgggcc tttcggactt
     8821 tggcgaagac tggttccgcg cgccgctgga cgccctgttg ggctcgatca acggcgaggc
     8881 ccggcttagc gctctgggca gaaccatcat ccgcaagcgc atcgtcgacg ctctcaccac
     8941 gcgtctgcgc gcccgggcgc tcctggacgc gtatccggaa attgagtcct tcgacccggg
     9001 cagggtgctg gtcatcgccg ggctgcagcg cacgggaacc acccttctgc atcgcctcct
     9061 cggcgccgat ccgcgcatcc gggcggtgct ctcgtgggaa gcgctcaacc cggtgcccct
     9121 gagcggcccg gcagagcgtg agcgcaggat ccgccaggcc ctgcgcgccg aacggggact
     9181 gaagtacatc gcgccggagt tctttgccat tcaccccgtc gagcacgaca tgcccgagga
     9241 agacgtgctg ctgctcgatg tctcgttcat gagccagtcc gccgaggcga ccatgcacgt
     9301 tcccacctac gctcgctggc tggaggagca cgaccacgcg cccgcctatc ggtatctcag
     9361 gcagcttctg agcgtgctgc tgtggcagcg ccacgcgccg accctgttgc tcaagtcacc
     9421 tcatcacctg gagcatctgg ataccgtgct cgacgttttt ccgaacgcga cgatcattca
     9481 aacccaccgc gacccgcagc agacggtgcc gtcgtttgcc agcatggtct gtcacggggc
     9541 ttcgatattc agcgatcggg tggacgtccc caggctcacc gatcactggc tccgcaagat
     9601 ccgtcgggtg gtggagcgct ccctgacggc ccgggatcga cacgggacgg cgcgcttcgt
     9661 ggacgtctcc tactacgacc tcatcggcga tccgatggct gaactcgctc gagtctatga
     9721 ggctgctggc ctggcctttg accctcaggc gcagcagggt gcccgcgaga cgttgcgccg
     9781 gcaggcccgc gaccgcttcg gccggcatcg ctatcgccgt gaggacttcg gtctgacccg
     9841 ggaactgatc gagggcgagc tggggttcta ccgcaggcgt ttcgacatcc ccttcgagga
     9901 agaggccgca tcatgaacgt caagccgacc acggacgcgc ccaacgccgt cgggcaccgc
     9961 ggcgtcgtga gcgcgcttgt cacggcgctg agcgatctgg tcggacgaag agatcagctc
    10021 gagccgatgc tgcctgacga gcggctggac ggtcggacat atctggtcac cggcgccagc
    10081 agcggcctgg gcaaagcggc ggccgtgcgc ctggcgagcg cgggcgcgga agtgatcatg
    10141 gcctgccgga gcgggattcc cgaagccggc gacgacgtca tgcgcgcgtc gggcagcggg
    10201 caggtcagga tgctggaggt ggatcttttc gacctcgaga gcgcctcggc catgtgcgac
    10261 cgtcttcggg atgacaagca gcacctcgac ggtgtcgttt tcaacgccgg gctcatgccg
    10321 ctttctgcgc ggcgcgggcc ccagggcttc gaggagatgt tcgccgtgca ctttctgtcc
    10381 agccggctca tcgtcgagcg gctgctggcg gacggtgtgg ttgcgccgag tgtcgacccg
    10441 gcgacccggc cacggctcgt gttcgtctcc tccgaggcgc atcggtctgc caggcccatc
    10501 gacttcgagc agtttggcgc gttcgtcgac tacggaattc gcggcggcat ggccgagtac
    10561 ggctacagca aactgcatct gtgcacgtat gccgtggagc tggcccgcag gctgagctcg
    10621 agcgacgggc cgggggtagc cgtgcacgcc ctgtgcccgg gcccggtggc ctcgaacatc
    10681 gccagggaag cgccgccctg gttgaagcct gtggttgggc ctatcatccg atggctgttc
    10741 gccccgcccg agcgagcggt ggagcctgtg cttctgctcg ccgccgggcg tgcagtggag
    10801 ggtcgtaccg gcgtttacct tcacatgatg cgcgaaaaga ccttctcatc gctggccgcc
    10861 gatcccgata acggccgccg cctgtgggag gcgagcggcg ccctgatcga gccgttcctc
    10921 aggcgctgag gacgaatccc tcgtagccgt gcttggctga gcggtcgcag tgatcgcggt
    10981 atttgcccat gccgcccacg taaggcatga aaacgcgggt tttgcccggg atgttcgcgc
    11041 cgacgtacca ggagttggcc agtggaaaca acgtcgcgtc gccttcctcg gcgacgtgcc
    11101 ggacccagcc cttctcggcg gcctgcgtcg tctcgatgcg tgagatgccg ttcgcgtaga
    11161 gatgct
//
LOCUS       DBNHLM010000372         6268 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1374625, whole genome shotgun sequence.
ACCESSION   DBNHLM010000372 DBNHLM010000000
VERSION     DBNHLM010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1374625"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="AC16QF_17265"
     CDS             complement(<1..560)
                     /locus_tag="AC16QF_17265"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6450362.1"
                     /translation="MRLSADEAERYARDGFLIRECAFTEQELSSLRLAAERAADAAWR
                     LSRSGRTYVLDGNRFVDAGHVTVQFEHAAGSETIRVVEPVAELDADLNALVDDARICE
                     PMRDLVGSEEIALWTDKLNLKRPREGSGFGWHQDSPYWIHECRHVDHLPNVMVTLDDA
                     SEANGCFRVVRGSHKRGCLPGRADGT"
     gene            complement(584..1744)
                     /locus_tag="AC16QF_17270"
     CDS             complement(584..1744)
                     /locus_tag="AC16QF_17270"
                     /inference="COORDINATES: protein motif:HMM:NF024863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR6450363.1"
                     /translation="MGSFTIAEIVPDRVCVRGALDFDHRPAGLSPRRLPAWTRPQLPR
                     EMDVMVRMPSGVRLAFTTDAAAIGLTFLATNMVMPPETRRPIPFDLEIDGALWSVASD
                     RGNTILLDRARPGAFELVKGDPDTVLFEDLPAGEKQCELWLPHNAYVELRELTLDDGA
                     FLAEPALDPRPRWVHYGSSISHCMEADHPGLIWPAVAARRAGASLTNLGFGGQCHLDQ
                     FVARTIREVDADVISIKTGINVINRDSMRERVFTPALHGFLDTVREGKPDTPIILISP
                     IYCPSAESRPGPTFPGRDGRFFTVSGHDEIREGCLTLRRVREIIEEVVEQRVAGGDRA
                     LAYVDGLDLFGAADAEDLPDDLHPNAAGYVRMGERFAPALADVLSSPETGGR"
     gene            complement(1752..2417)
                     /locus_tag="AC16QF_17275"
     CDS             complement(1752..2417)
                     /locus_tag="AC16QF_17275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6450364.1"
                     /translation="MEQLDLSNDDLLATTRAVRKRLDFDRPVEREALEECMELALQAP
                     TGSNAQGWHFVFVTDADKRGRIAELYQQAFSGYATSPMSAAAVHAGDPTMEQTQERVM
                     SSAQYLADNLHRAPVFLIPCIAGRFDGFSGEGANVAQASSFGSVIPAAWSFMLAARAR
                     GLGTCWTTLHLMHERKVADILGIPYEEITQVALIPVAYTKGTEFKRAPRKPMEGVVHF
                     ENW"
     gene            2565..2960
                     /locus_tag="AC16QF_17280"
     CDS             2565..2960
                     /locus_tag="AC16QF_17280"
                     /inference="COORDINATES: protein motif:HMM:NF024515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HIR6450365.1"
                     /translation="MRICGMGAAIFTLLLTGAASHAEMMAQSRAGHFLVTARSHLDPP
                     ATNRMHSWTLHVVDAAGQIVEDASIEVVGGMPDHDHGLPTAPRVTAYLGGGDYLLEGM
                     RFHMNGGWRIELTLRTDDVTDVAVFEIEL"
     gene            2963..4234
                     /locus_tag="AC16QF_17285"
     CDS             2963..4234
                     /locus_tag="AC16QF_17285"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR6450366.1"
                     /translation="MPGRVNTAILLAACLAIGGLVYRLAEPTGARWSEAELAIIESLW
                     LGNLGEAPPDPTNEVADSLAAAALGHRLFFDTRLSRDGTVSCATCHKPESYFTDDLRV
                     GIGAGVGTRHTMTVVGAAWSPWYFWDGRRDSQWSQALTPLESPAEHAGTRTEFVHLLA
                     DDAGYSAAYEQIFGPLPDLTDAARFPRRASPLGTPEQQDAWWSMAPADQTAVNEAFAN
                     MGKALAAYQRELRPGPGRFDAYAQALASGEEPSGSAALSAAEVAGLRLFIGDASCVNC
                     HNGPLFSNNEFHNTGVLPGVGQLPAMGRASGVREARSDAFNCLGPFSADPLRTCAELR
                     FAKSSDDLVGAQRTPSLRNVSRTPPFMHAGQIASLGEVLEHYRRAEPSLVGHNEAKPL
                     DLRQDQMRFLEAFLRTLDGDVAAEDRWLAPP"
     gene            complement(4245..5162)
                     /locus_tag="AC16QF_17290"
     CDS             complement(4245..5162)
                     /locus_tag="AC16QF_17290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6450367.1"
                     /translation="MSDVEFGIGLRRLDSAAEDARLAESLGYEFVTTGEHVFFHGPVG
                     NGLISLAAAAGATQRIRLMSTITLVPLYPPALLAKQVAALDVVSGGRFQLGVGVGGEF
                     PREFEACGVPVSERGARTNEALEVMKRLWTEDDVRFEGPFTSLPGVTLNPKPTQKPHP
                     AIWISGRSDAAMRRCARYGTGWLPYMYTPEMLHESLATIAQHSESVRRSEPVRPGLFI
                     FFAVHPDHDRAVAMAAERLSVQYNQDFTKLVGRYALAGDPAACIARLREYVDAGASTI
                     ILNSACPGDYVAENERLLAQEVLPALRAG"
     gene            complement(5159..>6268)
                     /locus_tag="AC16QF_17295"
     CDS             complement(5159..>6268)
                     /locus_tag="AC16QF_17295"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6450368.1"
                     /translation="FAGRMLADLGADVVKVEPPEGDITRLWGRVIGGVPGYYHQQNAG
                     KRNICVDLRAPGARDLVFELVREADVLIENYRADVMPRLGLAYETLKSVNPRLIMLSV
                     SGFGHGGPESHRPAYAPIVHAEAGLLDRQARRGDIPFRDLPLSVADTNASLHGLVGVL
                     AAVILRERTGLGQHLDIAMIDATIATDDQLHYDLEDSEDTGPLRADTWQTGAGPILIS
                     ADFRHLWRLLTGDLGLADPTHKEMDLETKIGLRRQAVAEFMEGLETWESVEAAMASMN
                     LAWGRVRNAATMREQPTVRARAAIVEVDDGAGGRRPIPQSPYRFSAARSGVRGPAPGR
                     GEHNLEVLGDWLGKTEADVEALVAGGVLKTGEEES"
BASE COUNT         1011 a         2181 c         2073 g         1003 t
ORIGIN      
        1 tgggtgccgt ccgcgcgacc cggcagacat cctcgtttgt gacttccccg caccacccgg
       61 aaacaaccgt tggcctcgct ggcgtcgtcg agggtcacca tcacgttggg cagatggtcg
      121 acgtgtcggc actcgtgtat ccagtagggc gaatcctgat gccagccgaa gccggagcct
      181 tcccgcgggc gcttgaggtt gagcttgtcc gtccacaggg cgatctcctc cgagccaacc
      241 aggtcccgca tcggctcgca gatacgcgcg tcgtcgacca gggcgttcag gtccgcgtcg
      301 agctccgcaa cgggctccac gacgcgaatc gtctcgctgc cggcggcgtg ctcgaactga
      361 acggtgacgt gtccggcatc gacgaaacgg ttgccgtcca ggacgtaggt ccttccgctg
      421 cggctcaggc gccaggcggc gtccgccgcc cgctccgccg caagccgcag tgacgagagc
      481 tcctgctccg tgaatgcgca ctcgcggatc aggaaaccgt cacgcgcgta gcgctcagcc
      541 tcatcggcac tgagccgcaa gcccctctga ctggacgccg gcgtcaacgg ccgccggttt
      601 ccgggctgct cagaacgtcg gcgagcgcag gcgcaaaacg ctcgcccatg cgaacgtatc
      661 cagcagcgtt cggatgcaga tcgtcgggca gatcctcagc atcggccgca ccgaagaggt
      721 cgaggccgtc aacgtaggcg agcgcccggt caccgccggc aacacgctgc tcgaccacct
      781 cctcgatgat ctcccgcacc cggcgcagcg tcaggcaacc ctcacgaatc tcgtcgtgac
      841 cgctgaccgt gaagaaccgg ccatcccggc cggggaacgt gggtccgggc cgggattcgg
      901 cgctggggca gtagatgggg gagatcagga tgatgggcgt gtccggtttt ccttccctga
      961 cggtgtccag aaacccgtgc agcgccggcg tgaacacccg ctcgcgcatg gagtcccgat
     1021 tgatcacgtt gattccggtc ttgatgctga tcacgtcagc gtccacttca cgaatggtcc
     1081 gggcgacgaa ctgatccaga tggcactgcc cgccgaaacc caggttcgtc aggctggcgc
     1141 ccgcccgccg cgcggccacg gcgggccaga tcagtccagg atgatctgcc tccatgcaat
     1201 gactgatgga gctgccgtag tgaacccagc ggggacgcgg atcgagggcg ggctctgcga
     1261 ggaacgcccc gtcgtcgagc gtcagctcac gcagctccac ataggcgttg tgaggcagcc
     1321 acagctcgca ttgcttttca cccgcgggca gatcttcgaa gaggaccgtg tcggggtcgc
     1381 ccttcacgag ctcgaacgcg ccgggccggg cgcggtcgag cagaatcgtg ttgcccctgt
     1441 ccgatgcgac gctccacaac gcgccgtcga tctcgaggtc gaagggaatc gggcggcgcg
     1501 tctccggcgg catcaccatg ttggtggcca ggaacgtcag gccaatggcg gcggcgtcgg
     1561 tggtgaacgc aagacgcacg cccgacggca tgcgcaccat cacgtccatc tcccgcggca
     1621 gttgcggccg cgtccaggcc ggcagtcgtc gcgggctgag accggccggc ctgtggtcga
     1681 agtccagcgc gccccggacg catacccggt ccggcacgat ctcggcgatg gtgaacgacc
     1741 ccaagcgagc gctaccagtt ctcgaagtga acgacgccct ccatgggctt gcgcggcgcc
     1801 cgcttgaact ccgtgccctt ggtataggct accggtatga gcgccacctg ggtgatctcc
     1861 tcgtagggga tgcccagaat atccgcgacc ttgcgctcgt gcatgaggtg gagcgtcgtc
     1921 cagcaggttc cgagcccgcg ggcccgcgct gccagcatga agctccacgc cgccggtatc
     1981 accgagccga atgagctcgc ctgggcgacg ttggctccct cgccggagaa gccgtcgaaa
     2041 cggccggcga tacaggggat cagaaacacc ggcgcccggt gcaggttgtc ggcgagatac
     2101 tgcgccgaag acatgacacg ctcctgggtc tgctccatgg tcgggtctcc ggcgtgcacg
     2161 gccgcagccg acatcgggct cgtcgcataa ccggagaagg cctgctggta gagctcggca
     2221 atccggcccc gcttgtccgc gtcggtgacg aacacgaagt gccagccctg agcgttggat
     2281 cccgtgggcg cctgcagcgc tagctccatg cactcctcga gcgcttcgcg ctccacgggc
     2341 cgatcgaaat ccaggcgctt gcgcaccgcc cgggtggtgg cgagcaggtc gtcgttgctg
     2401 aggtcgagtt gctccatgac ttggcattct ccggttcgtc gcgaaagatc ctgctttatg
     2461 cggcgacggt tttctccggt caagggcaat cgcccgggcc gggctttttt tgcgcgtctt
     2521 ccccgcctgc gctacgctgc cgcagcatgt acttggagtt ctcgatgcgc atctgcggga
     2581 tgggcgcagc catctttacc ctgttgctga ccggcgcggc ctcacacgcc gagatgatgg
     2641 cgcaatccag agccggccat tttctggtca ctgcccgaag ccacctcgac cccccggcaa
     2701 ccaaccgcat gcacagctgg accctccatg tagtggatgc ggcagggcag atcgttgagg
     2761 acgcctcgat cgaggtcgtc ggggggatgc ccgaccacga ccacgggttg cccacggccc
     2821 ctcgagtgac cgcctacctg ggaggcggcg actacctcct cgaaggcatg aggttccaca
     2881 tgaacggcgg ctggcgaatc gagctgaccc tgcgaacgga cgacgtcact gacgttgccg
     2941 tcttcgaaat cgagctgtag acgtgcccgg gcgcgtgaac accgccatcc tgctggccgc
     3001 ctgccttgcc atcggcgggc tcgtctaccg gctggctgag cccaccggtg cccgatggtc
     3061 cgaggcagag ctggccatca tcgagtcgtt gtggctgggc aacctgggcg aggcgccgcc
     3121 cgacccgaca aacgaggtcg ccgacagcct ggcagccgcc gcgctgggcc accggctctt
     3181 cttcgacacg cggctgagcc gcgacggcac cgtctcctgt gcgacctgtc acaagcccga
     3241 gagctacttc acggacgatc tgagggtcgg catcggcgcc ggcgtcggta cccgccacac
     3301 catgaccgtc gtcggcgcgg cctggagccc gtggtacttc tgggacggac gacgggacag
     3361 ccagtggtcc caggcgctca caccgctgga aagtcccgcg gaacacgcgg gcacgcgaac
     3421 ggagttcgtc cacctgctcg ccgacgacgc gggttactcc gccgcctacg agcagatctt
     3481 cggccccctt cccgacctga cggacgcggc gcgttttccc cgccgcgcga gcccgctggg
     3541 gaccccggag cagcaggacg cctggtggtc catggccccg gccgatcaga cagcggtgaa
     3601 cgaggctttc gccaacatgg gcaaggcgct agccgcctac cagcgcgagc tgcgaccggg
     3661 accgggccga ttcgacgcct acgcgcaggc gctggcgagc ggcgaggaac cctccggcag
     3721 cgccgcgttg tccgccgccg aggtagcagg cctgcggctg ttcatcggag acgccagctg
     3781 cgtcaactgc cacaacggcc cgctgttcag caacaacgag ttccacaata ccggcgtgct
     3841 gccgggggtg ggtcagctgc cggccatggg ccgggccagc ggcgtgcggg aagcgcgcag
     3901 cgacgccttc aactgcctgg gccccttcag cgccgacccg cttcgtacct gcgcggagct
     3961 tcggttcgcg aagtcgagcg acgatctggt cggcgcccaa cgcacccctt ctctgcgcaa
     4021 cgtgagccgg acgccgccct tcatgcacgc cgggcagatc gcttcgctgg gcgaagttct
     4081 cgagcactat cgccgggcag agccgtccct ggtcggtcac aacgaggcca agcccctcga
     4141 cctcaggcag gaccagatgc gcttcctgga ggccttcctg cgaacgctgg acggtgacgt
     4201 cgcggccgaa gatcgctggc tcgcgccgcc ctaatcgggc tgagtcagcc ggcgcgcagg
     4261 gcgggcagaa cctcctgggc gaggagccgc tcgttctccg ccacgtagtc tcccgggcag
     4321 gccgagttca ggatgatggt gcttgcgccg gcgtccacgt actcacgcag ccgggcgatg
     4381 caggccgccg ggtcgccggc gagggcgtaa cggccgacca gcttcgtgaa gtcctggttg
     4441 tactgcacgg aaagccgctc ggccgccatg gcgacggcac ggtcgtgatc ggggtgcacg
     4501 gcgaagaaga tgaacaggcc gggccgaacg ggctccgagc gccgcacgct ttccgagtgt
     4561 tgcgcgatgg tcgccaggct ctcgtggagc atttcggggg tatacatgta aggcagccag
     4621 ccggtgccgt agcgggcaca gcgacgcatg gccgcgtcgc tgcgcccgga tatccagatg
     4681 gcgggatgcg gtttctgcgt cggcttcgga ttcagcgtca cccccggcaa cgaggtgaag
     4741 gggccctcga accttacgtc gtcctccgtc cagagccgct tcatgacctc cagcgcttcg
     4801 ttggtgcgcg cgccccgctc gctcaccggc acgccgcacg cctcgaactc cctgggaaac
     4861 tcaccgccca caccgacccc gagttgaaag cggccgccgg ataccacgtc cagagccgcc
     4921 acctgcttgg cgagcagcgc aggcgggtac agcggcacca gggtgatggt gctcataagc
     4981 cggatgcgct gcgtggcgcc agccgctgcc gccagcgaga tgaggccgtt gccgaccggg
     5041 ccgtggaaaa aaacgtgctc gcccgtcgtg acgaactcgt accccaggga ttccgcgagg
     5101 cgggcgtcct cggccgcgct gtcgagcctc ctcagcccga tcccgaactc cacgtcgctc
     5161 atgattcttc ctccccggtt ttcaggacac cgcccgcaac cagcgcctcg acgtccgcct
     5221 ctgtcttgcc gagccaatct ccgagcacct cgagattgtg ctctccccgg cccggcgccg
     5281 ggccgcgcac gccgcttctc gccgctgaga accgatacgg ggattgagga atcggccgcc
     5341 ggccgccggc accgtcgtcc acctccacga tggcggcccg ggcgcggaca gtgggctgct
     5401 cgcgcatggt cgcggcgttg cggacgcgac cccaggccag gttcatgctg gccatggcgg
     5461 cctccaccga ttcccaggtt tccagccctt ccatgaattc ggccaccgcc tgtcggcgca
     5521 ggccgatctt ggtttccagg tccatttcct tgtgggtggg atcagccaga cccaggtccc
     5581 ccgtgagcag ccgccagagg tgacgaaagt ccgcggagat caggatgggc cctgcccccg
     5641 tctgccaggt gtccgcacgc agcgggcccg tatcctccga atcctccagg tcgtagtgga
     5701 gctgatcgtc cgtggcaatc gtggcgtcga tcatggcgat gtccaggtgc tggcccagtc
     5761 ccgttcgctc gcgaaggatg acggcggcca gcacgccgac cagcccgtgc agcgaggcgt
     5821 tggtgtcggc aacggacagc ggcagatccc ggaacgggat gtcgccgcgt cgggcctgcc
     5881 gatcgagcag ccctgcctcg gcgtgcacga tgggcgcgta ggccggccga tgagactccg
     5941 gcccgccgtg cccgaatccg gataccgaca gcatgatgag acgcgggttg acggacttga
     6001 gagtctcgta ggcgaggccg agacgcggca tcacgtccgc gcggtaattc tcgatgagaa
     6061 cgtccgcctc ccgaaccagc tcgaagacga gatcccgggc cccgggcgcg cgcaggtcga
     6121 cgcagatgtt ccgcttgccc gcgttctgct gatggtagta gccgggaacg ccgccgatga
     6181 ccctgcccca caggcgcgtg atgtcgccct cgggcggctc caccttgacg acgtcggccc
     6241 cgagatcggc cagcatgcgc ccggcgaa
//
LOCUS       DBNHLM010000373         3512 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1194710, whole genome shotgun sequence.
ACCESSION   DBNHLM010000373 DBNHLM010000000
VERSION     DBNHLM010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1194710"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..1454
                     /locus_tag="AC16QF_17300"
     CDS             291..1454
                     /locus_tag="AC16QF_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="steroid 3-ketoacyl-CoA thiolase"
                     /protein_id="HIR6450369.1"
                     /translation="MEDVYIVEAVRSPIGKRGKGLAGLMPADLLGAVQKAALERSGIA
                     PEKVGQVVGGCVSQVGEQSFNIARIAWLSQGLPEEVAATTVDSQCGSSQQATSLGAAM
                     VASGMENVVMCCGVEMMTRVPLGSNMKDGVPMGASYMEHYQPTSQFQGAAMIAEEYQL
                     TREDTDAFGLKSQQNAARAWQEGRFDREIAPVEAPVLDDSGQPTGETRVVERDEGPRE
                     TTLEGLQKLEPVPGQPIHTAGTSSQVSDGAAVVILANRKAVDELGLKPRARIVATTLV
                     GVDPVTMLKGPIPASRKVLDQAGLGVDDIDVFEVNEAFASVVLAWQKELNPDPERVNA
                     NGGAIALGHPTGSTGARLITTALHELERSGGRYGLIAMCCGGGLGTGTIIERI"
     gene            complement(1458..2186)
                     /locus_tag="AC16QF_17305"
     CDS             complement(1458..2186)
                     /locus_tag="AC16QF_17305"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6450370.1"
                     /translation="MFEEPNRRRIYLLRHAEAAYLDDDGNITDDVRAVGLTPTGRIQA
                     RKQAAVLASVEFDRAVCSGLPRTVETAKHILAYRDHPELETVSALEEIQGARGLAPQA
                     DVKAWLAMVANPWSGADHPEAQFLGGERFVDFAARVTPAFDAIVADHSWQTLLLVLHG
                     AVNRMLFNHIIGIPWSAKTSIEQDNCCINVIDVDTADDGATERFLIRGVNLTAYDLNK
                     SSFYLTHMEQAAQRMAGRVTGNAL"
     gene            complement(2179..3009)
                     /locus_tag="AC16QF_17310"
     CDS             complement(2179..3009)
                     /locus_tag="AC16QF_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6450371.1"
                     /translation="MTYTRMNVSAENGVGRIELNQPDEYNRMPLAFWRELPLALRKLD
                     ASGDVRALVLSSTGKHFSAGMDLSVFTGSARRDLDRGRAGEAGRRHIDRMQNVFNGLE
                     AVRMPVLAAVQGGCVGGGVDLVAACDARYCTADAFFCIQEINIGMAADVGTLQRLPRL
                     IPDGLMRELAYTGRRMHADEALSCGLVNAVYDSHEQMLEAVGAIAAEIASKSPLAVTS
                     TKHLLNYSRDHGVHDALAYQQLWMGAVTQGGEMAEYFKAKADGRDPVYADLPPLEDDV
                     "
     gene            3325..>3512
                     /locus_tag="AC16QF_17315"
     CDS             3325..>3512
                     /locus_tag="AC16QF_17315"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6450372.1"
                     /translation="MLTFADPLRRAVQIAAEDTAVIHDGARYSYAEFNRRCGQLVAVL
                     RELGIQRGDRVAILAGNC"
BASE COUNT          603 a         1071 c         1228 g          610 t
ORIGIN      
        1 tgagcagccc gccgatgatt gcgtttgtca cgactttccg gtcggttacg tgagggtggg
       61 cgatcttgcc acgaagagcg caacctgcct agttcccggc gtaacccggg atgagtcccg
      121 gtagtaaagt gtggccagga tctagagagg atctgcggag tgcagacgac tgacggtgcg
      181 gaaattctga ttgaggatcg gggttcgcga agctgagcgt ccggaaaccc gtggcgagct
      241 gccctggctc gggtagaatg cggcgctctc gtccagggag ttcgtaagca atggaagacg
      301 tctacatcgt cgaggccgtc cgcagcccca tcggcaagcg tggtaagggg cttgccggcc
      361 tgatgccggc cgatctgctc ggagccgttc agaaggcggc gctggagcgc agcggcatcg
      421 cgccggagaa agtcgggcag gtggtgggcg gctgcgtgtc ccaggtgggc gagcagtcgt
      481 tcaacatcgc ccggattgcc tggctgtccc aggggctgcc cgaggaagtg gcggccacca
      541 ccgtcgattc ccagtgcggc tccagccagc aggccacgtc cctgggggcg gccatggtgg
      601 cctccggcat ggagaacgtg gtcatgtgct gcggcgtgga aatgatgact cgggtcccgc
      661 tgggatcgaa catgaaggac ggcgtgccca tgggggcgag ctacatggag cactaccagc
      721 caaccagcca gttccagggc gcagccatga ttgccgagga gtaccagctc acccgtgaag
      781 atacggacgc gttcgggctg aagagccagc agaacgcggc ccgtgcctgg caggaagggc
      841 gcttcgaccg cgagatcgcg cccgtcgaag cacccgtgct cgacgattcc ggccagccca
      901 cgggagaaac ccgcgtggtc gagcgcgatg agggtccccg cgagacgacg cttgaaggat
      961 tgcagaaact ggagccggtg cccggccagc cgattcacac ggccgggacc agctcccagg
     1021 tctccgacgg cgcggccgtg gtgattctcg ccaaccgcaa ggcggtggac gagctgggtc
     1081 tcaagccgcg ggctcgaatc gtcgccacca ccctggtcgg ggtcgatccg gtgacgatgc
     1141 tcaagggtcc gatccccgca tcgcgtaagg tgctggatca ggcgggtctg ggcgtggacg
     1201 acattgacgt gttcgaggtc aacgaagcat ttgcctcggt ggtgctggcc tggcagaagg
     1261 agctcaatcc ggatcccgag cgcgtgaacg ccaatggcgg ggccattgca ttaggccatc
     1321 ccaccggctc caccggggcg cgcctgatca ccaccgcgct gcacgagctc gagcgcagcg
     1381 gcggtcgcta cgggctgatc gccatgtgct gcggcggcgg gctgggcacg ggaaccatca
     1441 tcgagcggat ctgaagctca aagcgcgttg ccggtcaccc ggccggccat gcgctgggcc
     1501 gcctgctcca tgtgggtcag gtagaagctc gacttgttga ggtcgtaggc cgtgaggttc
     1561 acgccgcgga tgagaaaccg ctcggtggcg ccgtcgtccg cggtgtccac gtcgatcacg
     1621 ttgatgcagc agttgtcctg ctcgatggac gtcttcgcgc tccacggaat gccgatgatg
     1681 tggttgaaga gcatgcgatt gaccgcgccg tggagcacga gcagcagggt ctgccaggag
     1741 tggtcggcta cgatggcatc gaaagcgggg gtgacccggg cggcgaagtc gacgaagcgc
     1801 tccccgccga ggaactgcgc ttccggatga tcggcgccgg accagggatt ggcgaccatt
     1861 gcgagccacg ccttcacgtc cgcctggggc gccagaccgc gggcgccctg gatttcctct
     1921 agagcagaga ccgtctccag ctctggatga tcccggtacg cgaggatgtg ctttgccgtc
     1981 tccaccgtgc gggggagccc ggaacacacc gcccggtcga actccacgct cgcaaggacg
     2041 gccgcctgct tgcgcgcctg gattctgccg gtgggcgtca ggccgaccgc gcggacgtcg
     2101 tcggtgatgt tgccgtcgtc gtcgaggtag gccgcttccg cgtggcggag caggtagatt
     2161 cggcggcggt tcggctcctc aaacatcgtc ttccagcggc ggcagatcgg cataaaccgg
     2221 atcgcgcccg tcggccttgg ccttgaagta ctcggccatc tcgccgccct gggtcacggc
     2281 gcccatccac agctgctggt aggccagcgc gtcatgcacg ccgtggtcgc gcgagtagtt
     2341 gagcaggtgc ttggtgctgg tgaccgccag cggagatttg ctggcgatct ccgccgcgat
     2401 ggcacctacg gcttcgagca tctgctcgtg ggaatcgtaa acggcgttga cgagcccgca
     2461 ggaaagcgct tcgtccgcat gcatccgccg gcccgtgtag gcgagctccc gcatgaggcc
     2521 atccgggatg agtcggggga gccgctgcag ggtgccaacg tccgcggcca tgccgatgtt
     2581 gatctcctgg atgcagaaaa aggcgtccgc cgtgcagtag cgggcatcgc aggcggccac
     2641 cagatccacg ccgcccccaa cgcagccgcc ctgaacggca gccagaacgg gcatgcgaac
     2701 cgcctccaga ccgttgaata cgttctgcat ccggtcgatg tggcggcggc ccgcttcgcc
     2761 ggcgcgtcca cggtccaggt cccgtcgggc agagcctgtg aagacggaga gatccatgcc
     2821 ggcactgaaa tgcttgccag tggaggagag aacgagggcg cgcacgtcgc cgctggcatc
     2881 gagtttgcgc agcgcgagag gcagctctct ccagaaggcc agcggcatgc ggttgtactc
     2941 gtccggctgg ttgagctcga ttcggccgac cccgttttcg gccgagacgt tcattcgagt
     3001 gtacgtcatg acagtctcgt tgcggttaac caagcatgtt accgattctg cccgcgggcg
     3061 gacaccgtcg gctgttggaa gatggcattg ccagcgccct ggaagccgct gataatcggc
     3121 cccgtgtcgg gcaacagata aggaggtgat cagtgtatct atctgcaacc tatgcagagg
     3181 aggggctggt ctcctgatcg gcttctcgag aacgggcccc tcaacggggc ccgttttttt
     3241 tggcctgcca ggtcgggcgt atttccacgg ccggaccttt ctgggagact ccggcgatct
     3301 gcgggaaatg tttccggggg aaagatgctg acattcgcgg atccgttgcg tagggccgtc
     3361 cagatcgccg ccgaggacac ggcggtgata cacgatggcg cgcgctacag ctacgcagag
     3421 ttcaatcgcc gctgtggcca gctcgttgct gttctgcgcg agctcggaat tcagcgcggt
     3481 gaccgggtgg cgatcctggc gggcaactgc aa
//
LOCUS       DBNHLM010000374        35329 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1313238, whole genome shotgun sequence.
ACCESSION   DBNHLM010000374 DBNHLM010000000
VERSION     DBNHLM010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 35329)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 35329)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35329
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1313238"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..480
                     /locus_tag="AC16QF_17320"
     CDS             106..480
                     /locus_tag="AC16QF_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HIR6450373.1"
                     /translation="MPANVPLAAGALFGLTGVVMGALGAHGLAGQLSESGVDIWDTAV
                     LYHLTHALALVAVGVRLGQKVPPPWPVVLAGWGFGVGILLFSGSLYVLALGGPPVFGP
                     LTPLGGLALCAGWVALLAAARR"
     gene            500..1180
                     /gene="trmB"
                     /locus_tag="AC16QF_17325"
     CDS             500..1180
                     /gene="trmB"
                     /locus_tag="AC16QF_17325"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF014447.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIR6450374.1"
                     /translation="MEERTYLRRRGRMTRGQARALDRAGDTMIVDSTSPDWPAVFGRQ
                     APLGVEVGFGMGHALLDWAAECPDWNLVGIEVYQPGIGALLLGLDSQGSTNVRVLEAD
                     ARQALAAGFEPDSIDEVRILFPDPWPKKRHHKRRLVQPDFVRLLASRMRPGARLGLAT
                     DWQDYAQHMLTVLDAEPLLQNRSGQGSYAERPSGRPTTRFEERGRRLGHQVWDLGYER
                     KRETTESR"
     gene            complement(1144..2247)
                     /gene="hemW"
                     /locus_tag="AC16QF_17330"
     CDS             complement(1144..2247)
                     /gene="hemW"
                     /locus_tag="AC16QF_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR6450375.1"
                     /translation="MGPVGLYAHFPWCVRKCPYCDFNSHPVRGSIDEAGYVSALLADL
                     RKNLPRDVPIGSVFFGGGTPSLFHAPAFAAVLEEVASQLTEDAEVTMEANPGTTEHSD
                     LRAYRQAGINRLSIGAQSFDDEALERLGRIHHAAETLDCAARARSAGFENINIDLMYG
                     LPHQTPDQAVQDLEAALALEPDHISWYQLTIEPRTEFARRPPPIPPEDRIDETEQRGR
                     DRLEQAGFERYEVSAYARPGRRSRHNVNYWSFGDYVGIGAGAHGKLTSGGRVARTVKP
                     SQPRLYLQDPESQQRQIVEAEALPFEFMLNALRLRDGVPLDQFTQRTGLALEALDPVW
                     GELVDEGLVHRHRLATTPEGYRYLDSVVSRFLS"
     gene            complement(2247..2834)
                     /gene="rdgB"
                     /locus_tag="AC16QF_17335"
     CDS             complement(2247..2834)
                     /gene="rdgB"
                     /locus_tag="AC16QF_17335"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR6450376.1"
                     /translation="MSIVLASNNPGKLAELRLLIAPLGLDAVSQRDLGIASAPETRAT
                     FIENALEKARHVSAAAGMPAIADDSGLAVPALGGAPGVRSARYAGEDATDAENNAKLV
                     AAMADMDDRRASFHCAVVFMRAPDDPVPVVATAAWSGRIVADPRGTGGFGYDPHFFID
                     ETDCTAAELDPSVKNEISHRGRAMRALCAALAGVV"
     gene            complement(2842..3279)
                     /locus_tag="AC16QF_17340"
     CDS             complement(2842..3279)
                     /locus_tag="AC16QF_17340"
                     /inference="COORDINATES: protein motif:HMM:NF025822.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4426 domain-containing protein"
                     /protein_id="HIR6450377.1"
                     /translation="MVKTGAGWICALILSWAGTPGHAEQRQSFGGWDVHYVVLPTGFL
                     KPAIAAQYGITRGRDRALVNISVLDPTGAPQKVSIEGTVKNLLGQLQPLAFREVTEGQ
                     AVYYLAAVKHTHEEVLRFEISLSAPDAPPMQLKFQQKLYWEDT"
     gene            complement(3279..3887)
                     /gene="metW"
                     /locus_tag="AC16QF_17345"
     CDS             complement(3279..3887)
                     /gene="metW"
                     /locus_tag="AC16QF_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008364885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HIR6450378.1"
                     /translation="MRPDLEIIAEVIKPGARVLDLGCGEGELLAYLQTSKQVNGYGLE
                     IDPDNITTCLHNGVNVIEQDLDEGLDNFPDDSFDLVVMTETLQAVQNPDRMLDDMLRI
                     GGECIVTFPNFGYWRCRAYLALRGRMPVSEHLPHAWYDTPNIHLCTFKDFERLCHSKG
                     LPVIDRFVVDERHLARRLMQRFPNFFGHIAFYRLGRPNSGEA"
     gene            complement(3877..5031)
                     /gene="metX"
                     /locus_tag="AC16QF_17350"
     CDS             complement(3877..5031)
                     /gene="metX"
                     /locus_tag="AC16QF_17350"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase MetX"
                     /protein_id="HIR6450379.1"
                     /translation="MSSSLPSDSVGLVSPQVFAHPSGFSLRCGETLEGFELVYETYGR
                     LNAARSNAILVCHALSGSHHAAGYHSEDDKRPGWWESCIGPGKPIDTDRFFVVCPNNL
                     GGCHGSTGPTTVDPRTGEPFGPNFPVVTVEDWVDTQALLADHLGIDRFAAVVGGSLGG
                     MQAMRWATDYPDRLHAAVLIACAARLSAQNIAFNEIARQAIISDPEFHGGNYRQLGVN
                     PDLGLMLARMVGHVTYLSDDGMRQRFGRQLKSGDIETAQDVEFQVESYLHHQGRSFSQ
                     RFDANSYVLMTRALDYFDPAREHDDDLVKALASATCRFCVISFTTDWRFSVARSKELV
                     DALIAAGKNVASAIIESEHGHDSFLLNVPRYFDVLRAYLGRIATEAVDAP"
     gene            complement(5132..5668)
                     /locus_tag="AC16QF_17355"
     CDS             complement(5132..5668)
                     /locus_tag="AC16QF_17355"
                     /inference="COORDINATES: protein motif:HMM:NF014389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR6450380.1"
                     /translation="MAEVLYYATSFILQVIAFLFVVRFLLQACRADFYNPISQGIVKA
                     TDVVLKPVRMVLPGYRNLDFAALLAALIVEVVMILALGAIADRPTGTAVQIATSGVLQ
                     MILLGLFIIKWSILIVIIASFLAPGNYHPALALLQQLTEPVLAPARKLLPPMGGLDFS
                     PILVFLILGVIERVLLQI"
     gene            complement(5699..6508)
                     /gene="proC"
                     /locus_tag="AC16QF_17360"
     CDS             complement(5699..6508)
                     /gene="proC"
                     /locus_tag="AC16QF_17360"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR6450381.1"
                     /translation="MSSHITFIGAGNMATSLIKGLIASGHDAATIRASDPAPEQLGKI
                     AELGVATSDDNNAQVRGADVVILAVKPQVMASVAGGLTALSTDQLLVSIAAGVPMSSL
                     AGWTSSEQPIVRCMPNTPALLGAGVTALCANERVSPAQRELAESVLATAGRTLWVSLE
                     DQLDAVTAVSGSGPAYFFYLMEAMIEAGQALGLSAETAVTLTLETARGAALMALEGPP
                     PAQLRANVTSPGGTTQAALEILDAADCRGIINAAVAAAAERSRELAIEFGR"
     gene            complement(6542..7219)
                     /locus_tag="AC16QF_17365"
     CDS             complement(6542..7219)
                     /locus_tag="AC16QF_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR6450382.1"
                     /translation="MHSISHNVASVRSCVVSAARAAQRDPDEVCLIAVSKRQPARAIE
                     EAHGAGLKDFGENYLQEALPKIAALGHLPLTWHFIGALQSNKTRQVARHFDWVHTVDR
                     PKIARRLNEQCPDDKTLQVCLQVNIDADPGKAGVDPAAVADLLKELVDLERICVRGLM
                     TILDPNADAPESFARMAKLFAGLAPLAAGRWDTLSMGMSNDYEQAIMAGATHVRIGTA
                     IFGAREG"
     gene            7385..8419
                     /locus_tag="AC16QF_17370"
     CDS             7385..8419
                     /locus_tag="AC16QF_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR6450383.1"
                     /translation="MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEH
                     QQVHSLIYEIMNDKQRKDFEEFLETDFSVEVPGVARFRVNAFNQNRGAGAVFRTIPSK
                     VLTMEDLDMGQVFRDICLVPRGLVLVTGPTGSGKSTSLAAMVDFINDHRYDHILTIED
                     PIEFVHESKKCLVNQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAA
                     ETGHLVFGTLHTTSAAKTIDRVIDVFPAEEKSMVRSMLSESLQSVISQTLLKRVNGGR
                     VAAHEIMIGTAAIRNLIREDKVAQMYSAIQTGSSVGMQTLDQCLLDLVQKRTITRDSA
                     REKAKNPDDF"
     gene            8437..9576
                     /locus_tag="AC16QF_17375"
     CDS             8437..9576
                     /locus_tag="AC16QF_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HIR6450384.1"
                     /translation="MQFEKLLKLVVEKGASDLFITAGKPPMLKVNGKLFAAGTTNLSA
                     DQSRDVVHSIMDEDQKKQFHDHHECNFAIHSEGLGRFRVSAFQQRNLAGMVLRRIEVN
                     IPLVSDLGLPPILEDLSMTRRGLIIFVGATGTGKSTSLAAMIGHRNEHSSGHIISIED
                     PIEFVHQHRGCVVTQREVGVDTESFEVALKNTLRQAPDVIMIGEVRTRETMEHAVAFA
                     ETGHLCLCTLHANNANQALDRIIHFFPSDRHSQVWMDLSLNLRAMVAQQLVPTVDGQG
                     RRAAIEILINTPLMADHIRKGEVHLLKELMSKSTEHGMQTFDQALYQLYSEGEISHEA
                     ALQHADSPNDLRLMIKLGSDANPDLMGQVEGLTIEEDEPAGTFLR"
     gene            complement(9598..9987)
                     /gene="ruvX"
                     /locus_tag="AC16QF_17380"
     CDS             complement(9598..9987)
                     /gene="ruvX"
                     /locus_tag="AC16QF_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR6450385.1"
                     /translation="MKQGHVLAFDFGLKHIGVAVGQSVTRTASPLITIAARDGTPDWS
                     DVERLITEWQPAVLLVGLPLNMDDSESEMSLKARGFAERMGRRFGVPIELVDERLTSF
                     AARESGSDRPHEAAATMIAESWLAADG"
     gene            complement(9995..10555)
                     /locus_tag="AC16QF_17385"
     CDS             complement(9995..10555)
                     /locus_tag="AC16QF_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003297209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR6450386.1"
                     /translation="MAADTSLKNQFLLAMPGLAGTYFGDTVTYVCEHSADGAMGLMVN
                     RPTEVTLQALLADLGIDSSAVSADIPVMEGGPVASERGFVLHSDDRAFDSSLSLGDGL
                     MLNSTREVLEAIAAGEGPADFLVAMGYAGWGGGQLEQELLENAWLTCPGNTDVLFRTP
                     FAERVNQAAAALGIDFRLMSSQAGHA"
     gene            complement(10545..11405)
                     /locus_tag="AC16QF_17390"
     CDS             complement(10545..11405)
                     /locus_tag="AC16QF_17390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6450387.1"
                     /translation="MSAAAITPRDRLGFTLFLAVSLHAAIILGVSFTADLDLLESPTV
                     EVTFAQHSDLEAPEDADFIAQTNQLGSGTESEVLETTTDQEAEFFANEFADAISNPLP
                     TIEQNQLDSRELVTTISEVDDVAPIEEETPVEENVNPQPATDIRFDDLAKEIASLEAR
                     IAADQQAKAKQPRVKRLTSVSAKSADEAAYLNMWRQKVERIGNANYPDSRVYGNLRML
                     VVLSYDGTLKDVRILESSGHKSLDDAALRIVRLAAPYQQFPVEMRKKYDQLEIIRTWK
                     FSRSGARLGS"
     gene            complement(11430..12416)
                     /gene="gshB"
                     /locus_tag="AC16QF_17395"
     CDS             complement(11430..12416)
                     /gene="gshB"
                     /locus_tag="AC16QF_17395"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004419666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HIR6450388.1"
                     /translation="MSLKIAFVMDPLPTLSLKKDSTLAMIRAAQLKGWQVGYLEQGDL
                     LLHEGAPCGMLRSLELVGPFADSLDPAEAGDRWYQLSEAALTPLAELDVIMMRKDPPF
                     DMEYIYSTYLLERAEALGVLVVNKPQGLRDCNEKFFATAFQECCPPLVVTRSEAALRE
                     FQRTHGNVVFKPLDGMGGQGVFRIMEGDENLGVIIETLTKGGTAQIMGQKFIPEIASG
                     DKRVLLIDGEPIPYALARIPMAGEARGNLAAGGTGEGRELTERDRWIAAQVGPELRRR
                     GLLFVGMDVIGDYLTEVNVTCPTCIRELDAQFSLNIGGMLMDTISAKLDAQA"
     gene            12672..13052
                     /gene="pilG"
                     /locus_tag="AC16QF_17400"
     CDS             12672..13052
                     /gene="pilG"
                     /locus_tag="AC16QF_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HIR6450389.1"
                     /translation="MEQELKGVKVLVIDDSKTIRRSAENLLTKEGYDVITATDGFDAL
                     AKIVDSRPGIIFVDVMMPRLDGYQTCALIKNNSEFKTTPVVMLSSKDGLFDKAKGRIV
                     GSDQYITKPFSKDELLGTIRNLVH"
     gene            13078..13443
                     /locus_tag="AC16QF_17405"
     CDS             13078..13443
                     /locus_tag="AC16QF_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010795388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6450390.1"
                     /translation="MARILVVDDSPSETHQLANALSRHGHQVLTAASGSDGISLANEE
                     QPDIILMDVVMPGINGFQATRQLTRSQGTSHIPVIIVSSKSQDADRVWGKRQGAQGYL
                     TKPVDDRTLISTVNEALSA"
     gene            13452..13994
                     /locus_tag="AC16QF_17410"
     CDS             13452..13994
                     /locus_tag="AC16QF_17410"
                     /inference="COORDINATES: protein motif:HMM:NF013731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR6450391.1"
                     /translation="MNGQAQNILTDIRATTIANAAPLPSRENEHLEWQGVGYQLGGVR
                     LVSPMGEISEILAVPKFATLPGVKPWMLGVANVRGRLIPIIDLHNFLDMRTTTPMVQW
                     RVLVVEDEELIAGFVVEQSLGIQHFLEDSFEESTAEEVAALKPYLRGAFHQGGCVFYE
                     AHLKSILRDEKFFDVADKTN"
     gene            14139..15242
                     /locus_tag="AC16QF_17415"
                     /pseudo
     CDS             14139..15242
                     /locus_tag="AC16QF_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851231.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            15252..21149
                     /locus_tag="AC16QF_17420"
     CDS             15252..21149
                     /locus_tag="AC16QF_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR6450392.1"
                     /translation="MATSRENFALDWVKGALQESLDEAIAALDSFAEAGHGEAQMREC
                     MTALHQVHGALVMLELKGTTLLAEHLERLAQHLMIGAVGDSVGAGQCLMQGLLELPGY
                     LDEVQRGLSDAIEPMIPLINEVRGHLDERPLEGAMVTDLGVASEAAIERFDGIDGVEK
                     AGRIRAAYQQVLLCILRGDDRERAVITLRKIAEVLSRVCLGTPHETQWRALGEFAASL
                     ATDTGPIDPDAAKLLRRIDSEIRTLSRDGLGVAGSPVSLDLIKQLLDACRQRGYRSEA
                     ITDISNALAGRGDGGGLAMNGLKALVSASLVVREELALVKDQLDLFARARGRASVDLN
                     SLLAPLKQIRSTLTLLGFASAHEIVTQQLEALEQLASAEAVDPQDLFSVASALVRVDE
                     SMASFTEGDQEVEKITNDAQRTVTVEVRNGLEPVRQAVLEYIAAGWDSEPLAGVPEQI
                     AALCGALEMIPLRRAARILSSCGAYLRRDLLEAGATPGMDAMETLADAITAVDYYLQR
                     TAEDITGGIEDILDVADASLEKLGYAGIVDERPAAQPGEAFQAETAGVEETAEEGFDW
                     EGFVSEAADLREEPASSDAQDQEAPESEPELIDFEPVALEGGSDDEPEAAQAASADAE
                     PEAEPEDASAVEETPVAASAPSPALPPLSPAIAEKTSRLADAFESDEEIVEIFVEEVD
                     EVLETINGCLPRWGEHLSDEDSLTELRRAFHTLKGSGRMVGANIIGELAWSVENMLNR
                     LIDGTFEPTGQFALVVEEATSLMPYLRDAFEAQRAPDLDAMGRIMEKADVLASGGSLE
                     EQAPVAEEEPAVEAAEAGEGFEALGLFVSESEEHLAVLALCDDGEDFVLDEDAYRALH
                     TLAGSAGMAGIEVVSELARPTYEVALAARSEQEVTRASGAVAEFLRAATTALACMIET
                     LRAGSEPEEELQLIADADRLLVEIQETGESKLDELMKLPALAVVMDAEEFLGGWARGV
                     MDLEFAGKLIGALGEIADLARATERSAVADLAAALRATHEELEQETISPESHVALSRA
                     HETLLQQFDAMAAQQTVALATAEIASLTSLREAVTEPSSVEAFGTGAGSSAGSDYAEG
                     AGSRGAEDVAAGDTGTPAEPPAEAGPRVASLPIASLLPEEVDEDIIGIFFEEAEEILE
                     SLDNSIHEWTGEPENRLFLENLLRGLHTLKGGARLSGLSVLGDYTHEFETFLIDFQLS
                     SAPLPEGFFTDLGARYDELEILVSRIRKAIESGERGESFAEAPASATSTPPAEPVAEP
                     ERVAEEDTTGAAAPTDEQPQPAEEAPAQASEVPDLAAAEASAEEAEAEELPAQEIAAS
                     EETDEAEPEPVAEPEPVAAPAPVLRVAPEPSEEAPSAPDPTRADRAKEMVRVGAGLLE
                     ELVNLAGESSIVRARIEQGMSDFTGALDEMETTIERVREQLRRLEIETEAQILFRRDT
                     LEGPGYDEFDPLEMDRYSQLQQLSRGLSESASDMLDLKDTLLFKARESETLLLQQARI
                     NTELQEGLMRTRMVPFGRLLPRLRRIVRQVSRELGKDVELHAHNMEGELDRNLLERMV
                     PPLEHMLRNAVDHGIESAEERRRAGKPNTGRIDIRLTREGGDVVIEIADDGAGINVDS
                     VRAKAIERGLMAENSVLGDEEICQFVLAPGFSTAKSVTQISGRGVGMDVVHSEVKQLG
                     GSINIVSRARKGSRFVVRVPFTVSVNRALMVSVGEDLYAIPLNTIEGIVLLSAEELDK
                     VHPPLSKPFEYAGVRYNVRYLGSYLGREYRGNPSQAAVPVVLVRSGDRAVAVHVDAVQ
                     GSREIVVKSLGPQFAGVGGVSGATILGDGSVVVILDLLALIRAQKGDGLTVTRRVMPG
                     AAKTRCVMVVDDSVTVRKVTSRLLERQGMDVLVAKDGIEAIAMLQERRPDVMLLDIEM
                     PRMDGFEVARQVRRDERVQDLPIVMISSRTGEKHQEHAAELGVNRFLGKPFQENELLA
                     TIDSLVN"
     gene            21160..21651
                     /locus_tag="AC16QF_17425"
     CDS             21160..21651
                     /locus_tag="AC16QF_17425"
                     /inference="COORDINATES: protein motif:HMM:NF013731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR6450393.1"
                     /translation="MEPATAQELIEMSCVMIPLQETNLLLPNICVAEILPWRRVSSIE
                     DAPKWCLGMLEWRGEVIPVVRFERMGDASVEDAEEGRCLIVMNRSRTIEGLPFYALAA
                     EGLPRIVQLTAEDMSNVPTNLGVSETMALRVGSEPAIIPNLQYLEVQVANLVRGQETA
                     KQA"
     gene            complement(21648..22367)
                     /locus_tag="AC16QF_17430"
     CDS             complement(21648..22367)
                     /locus_tag="AC16QF_17430"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIR6450394.1"
                     /translation="MSRRIYLEASLLPGATVRLDPDHSHYLTRVMRLKAGDALRCFDG
                     SGREHAARLAEPNPRSCSIEIAEELRNAAPATVRLHLAQAWLKGQAMDLVIQKATELG
                     VTDIWPIEALRSNVRIDAGRTAGRLMHWRGIAKSATEQSERLFLPRVHEPRRLEALLT
                     ERPCQRLILLDPGAAALPGALPFDSLVLMVGPEGGWTEHERELAQAAGADRYGLGDLV
                     LRAETAPIAALAAVRQSWGWR"
     gene            complement(22364..23221)
                     /locus_tag="AC16QF_17435"
     CDS             complement(22364..23221)
                     /locus_tag="AC16QF_17435"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR6450395.1"
                     /translation="MPERGQRPRLSFEFFPPRSEEGFASLARVTAKLDELRPDFYSCT
                     YGAGGSTRDGTKQTVERLVSSGHATAPHLSIGADEEETTFAMLDGYRGMGIDRIVALR
                     GDVPSGIGRSRFRANAQALVGRIRKHSGSHFHIEVAAYPEVHPDARSPGSDLEFFRRK
                     VDAGADSAITQYFYSPHAYYDFVDRCAGAGVTIPIYPGIMPITSYEGLIRFSDNCGAD
                     VPRWLRKHLEAFGDDDDGLRRFGIETVSRLCDDLLRAGAPGLHFYTLNRWGASRAICQ
                     NLGLANRDG"
     gene            complement(23247..24401)
                     /gene="metK"
                     /locus_tag="AC16QF_17440"
     CDS             complement(23247..24401)
                     /gene="metK"
                     /locus_tag="AC16QF_17440"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007021908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR6450396.1"
                     /translation="MNEYSIFTSESVGEGHPDKMADQISDAVLDAMLEQDAESRVACE
                     TLIKNNLVVLAGEIRTDAWVDVDRLARQVISDIGYGSHDEGFDPWDCTVMNALGVQSS
                     DIAMGVDETSEHEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQKQAEVRRSSLKF
                     LRPDAKSQLTFRYDEAGNPAAIEAIVLSTQHSPDVDQKTIAEAVMEEIIKPVVPSNWI
                     SKDTSIFINPTGQFVIGGPVGDCGLTGRKIIVDTYGGVARHGGGAFSGKDPSKVDRSA
                     AYAGRYVAKNIVAAGLADRCELQVSYAIGVAEPTSISINTFGTGKLDDERIIQLVREH
                     FDLRPKGLIDMLDLKRPIYRNTAAYGHFGRSEPEFTWERTDKAEALAAAL"
     gene            24632..26614
                     /gene="tkt"
                     /locus_tag="AC16QF_17445"
     CDS             24632..26614
                     /gene="tkt"
                     /locus_tag="AC16QF_17445"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR6450397.1"
                     /translation="MVTRTDRANAIRALAMDAVQAANSGHPGAPMGLADIAEVLWRDF
                     LKHNPADPAWANRDRFVLSNGHGSMLLYSVLHLSGYAVTMDDLRSFRQLHSRTPGHPE
                     YSECPGVETTTGPLGQGLANAVGMALAEERLSRRFNRDGFSVVDHRTWVIVGDGCLME
                     GISHEAASLAGTLQLGKLICIYDDNGISIDGDVKAWFTEDVPARFEAYGWRVIRDVDG
                     HDPNSIQRALDDAVSRQDRPTLVCCRTVIGFGAPNKQGTADVHGSALGEEEVANARRE
                     LGWAAPPFEIPEPVYASWDCREKGAEQQRSWEALLAEYRAAHPEDAAELERRVSGDLP
                     EGWEGPIRALAEASQEAMEPLETRKSSQRCLAALAGALPELFGGSADLTGSNGTRWDG
                     ADEDRYLSFGVREFGMTAITNGMALHGGFRPFSGTFLVFMEYARNAVRLAALMKIPNI
                     FVYTHDSVAVGEDGPTHQPIEQLTNLRTTPGLSTWRPCDTVESAVAWEAAVASRQRPT
                     ALIFTRQKTAPQPRSAQAFADIRRGGYVLKAEAGTLAAVVIATGSEVEIAVAAAASLE
                     SDGVGVRVVSMPSTDVFLSQDRAYQDAVLPPALRARVAVEAAHPDYWYRFVGLDGAVV
                     GIDRFGLSAPGGQAMAALGITADNVAEAVRSVLKEA"
     gene            26622..27785
                     /locus_tag="AC16QF_17450"
     CDS             26622..27785
                     /locus_tag="AC16QF_17450"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR6450398.1"
                     /translation="MSQRRNMTDLDLAERRVLIREDLNVPIRDGRITSDARVRAALPT
                     IEAAMRAGARTLVMSHLGRPVEGEFDPALSLAPVAGRLGELLGVDVPLVGLDDVGVID
                     PGAVSLLENVRFLKGEGADDEALARRLASLCDIYVMDAFATAHRAQASTHGVAKFAPV
                     ACAGPLLAGELEALERALRAPARPMTAIVGGAKVSTKLEVLDTLATIADRIIVGGGIA
                     NTFIAAAGHPVGRSLYEEDLLEAARSIAAKVEVPLPVDVMVGERLDAGAVAVLRGLDE
                     VRPDEMILDIGPETARRYAAELGSAGTILWNGPLGVFEFDQFGEGTRVVAQAIASSGA
                     FSIAGGGDTLAAIDKYDVRDGVSYISTGGGAFLEFVEGKTLPAVAVLASRAEG"
     gene            27799..28758
                     /locus_tag="AC16QF_17455"
     CDS             27799..28758
                     /locus_tag="AC16QF_17455"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6450399.1"
                     /translation="MEVGFDPDSLRQALKPLVPGSPTVLTPQEDAYLRHYGIHFVEEY
                     PDLAHHFGAVETDSHSVAAHLWSPRDARGTAVIVHGYYDHTGLYGHLIRHLIDRGLAV
                     LSFDLPGHGLSTGAPATIETFDHYVAAFDACIAALGAHLPKPWHLLGQSTGGAVAMEW
                     LLANGVARATSPFSGIVLLAPLVRPYLWPVGRLFYEVVRRFITERERTFSVNAENPEF
                     IEFLKHHDPLQARVLPVQWVTAMVAWKKRFVSRAPSDLGLLVVQGTADRTVDHRYNLK
                     IIEQLFDAKVVYVPEARHHLVNESVVLRQQIFQAVDAQLDAAG"
     gene            28766..30142
                     /locus_tag="AC16QF_17460"
     CDS             28766..30142
                     /locus_tag="AC16QF_17460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013974677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6450400.1"
                     /translation="MTSAHGQLTSALSPSQVRTDPDSLHEWGRDWTRTAEPAPAAVVF
                     PETTADVVAIVRLANSEGLKLVPSGGRTGLSGGAVAADGEVVVSFDRMNRVLEFSAAD
                     RLLSCQAGVVTRTVQDFARERGLSYPVDFASSGSSQIGGNIATNAGGIKVIRYGLTRD
                     WIAGLRVVTGAGELLDCNRGLVKNATGYDLRHLFVGSEGTLGFIVEADVRLAPAPAPQ
                     TVMLLGVTEFPRLIDVLSVLQSTVVLSAFEFFSELALQKVLSHRKLRRPLAETAPFYA
                     LVEFDTADLEPATAAYEAAVGGGWVCDGVVSQSEGQAAALWSLREGISESIAQFTPYK
                     NDLSVRVSVLPEFLAQVDGLVAQSYPGFEVCWYGHIGDGNLHLNILKPGGLSLAEFYA
                     RCHAISPSLFELVARHGGSVSAEHGVGLLKRDFIGYSRSAAEIEIMRALKGVLDPNQI
                     MNPGKLLS"
     gene            complement(30177..30842)
                     /gene="rpiA"
                     /locus_tag="AC16QF_17465"
     CDS             complement(30177..30842)
                     /gene="rpiA"
                     /locus_tag="AC16QF_17465"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HIR6450401.1"
                     /translation="MDQDQLKRSAAEAALAYIEPKLGSASIVGVGTGSTTNHFIDALG
                     AIRHKFDAAVASSEASAARLKAVGIPVIDLNAAAAVAVYVDGADEVNAARQLIKGGGG
                     ALTREKIVAASADEFVCIVDESKVVQTLGAFPLPVEVIPMARGLAARAIVGLGGAPEL
                     REGFVTDNGNIILDVYELTITDPAAMESAVNNIVGVVCNGVFAAQAADLVIVAGASGV
                     TSF"
     gene            30915..32426
                     /gene="ilvA"
                     /locus_tag="AC16QF_17470"
     CDS             30915..32426
                     /gene="ilvA"
                     /locus_tag="AC16QF_17470"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HIR6450402.1"
                     /translation="MEKYVKKILSSRVYEAAVETPLQPARNLSIRLGNHVRLKREDMQ
                     PVFSFKIRGAYNRIVQLTEEERGRGVIAASAGNHAQGVAYSAQQLGIKALIVMGRNTP
                     SIKVNAVRALGAKVVLHGDAYDEAAAHALKLAERNGYVFVHPYDDPDVIAGQGTVGVE
                     ILNQHVAVPDAIFVPVGGGGLIAGIAAYVKYLAPDTRIIGVEAEGSACLAAAMAAGRR
                     VRLPFESLDLFADGVSVAQIGKETFKVARRCVDEVITANTDEICAAIKDLFDDTRSIA
                     EPAGALAVAGLKKFVEREGVRNLNLVAVVSGANVNFDRLRHISERAEVGEHREVVLGV
                     TIPEKPGSFLKFCKMLGNRPVSEFNYRFATDREAQVYVGLQVSAADDRAELMKSLSTG
                     YVVHDMTDNEAAKLHVRHMVGGRTFGSKPELLYRFEFPERPGALLRFLTGLGSRWNIT
                     MFHYRNHGAAWGRVLVGFEAQPRDRRSLGEYLKRIGYRYWEETDNPAYQLFLR"
     gene            complement(32433..32966)
                     /gene="def"
                     /locus_tag="AC16QF_17475"
     CDS             complement(32433..32966)
                     /gene="def"
                     /locus_tag="AC16QF_17475"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR6450403.1"
                     /translation="MAVRKIIRMGHPTLRRVARPLAGEEIRSEAVGRLIGDMIDTLHD
                     YGGIGLAAPQINESVRLAIIEIPGGPTRYGEIPALPLTVFINPSVEVVDPATAGYWEG
                     CLSVPGLRGFVERPQHVRVNALDQEGKPLDLELKGFLATVFQHEFDHLDGRLYVDHLK
                     DPALLAFDEEFERYLAE"
     gene            complement(32986..33570)
                     /locus_tag="AC16QF_17480"
     CDS             complement(32986..33570)
                     /locus_tag="AC16QF_17480"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR6450404.1"
                     /translation="MTSTLERQELRRFFRERRRALGSHQQREHALRVARHLLNNPVSW
                     RSRRIGAYWANDGEVSLAPLLGELEYRNRQLALPVVSTAGRMAFYRYHRAAPMLVNRY
                     GIPEPGPGSAFVDGRSLDLILLPLVAFDEYGVRLGMGAGYYDRFLGALPEPLRPRIVG
                     IAHEVQRSADPLPFQDWDIPLDGIVTERGWQAFT"
     gene            complement(33592..33802)
                     /gene="ssrS"
                     /locus_tag="AC16QF_17485"
     ncRNA           complement(33592..33802)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC16QF_17485"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(33875..34189)
                     /locus_tag="AC16QF_17490"
     CDS             complement(33875..34189)
                     /locus_tag="AC16QF_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR6450405.1"
                     /translation="MSEEATTVSVKILDKEYQVSCPAEEVDALSRSARYLDEQMRSIR
                     ESGKIFGMDRIAVMAALNIANEFINSSKSLESTHGVVSQKVTELTERVGRALSEQKQL
                     NL"
     gene            complement(34203..34409)
                     /locus_tag="AC16QF_17495"
     CDS             complement(34203..34409)
                     /locus_tag="AC16QF_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HIR6450406.1"
                     /translation="MADTEWTALESKVDELIELCSVLTKENRALRAQQQNWTTERAKL
                     IEKNELAKARVESMITRLKALEQD"
     gene            34531..35073
                     /locus_tag="AC16QF_17500"
     CDS             34531..35073
                     /locus_tag="AC16QF_17500"
                     /inference="COORDINATES: protein motif:HMM:NF015642.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HIR6450407.1"
                     /translation="MADLTALALGAVESLSVAEIHGAVCGLAVASEGGIELGALVELV
                     GIEALRDEHSVGEFVNAAVDALHAEDLSFAPLLPDDHVDLALRLEALGEWCGAFLAGF
                     ASGSRFQELGEEQIPEEAREIIGDFTYIANVDGSEAGDGEDPESAERDYAEVHEYVKV
                     GALLLRTLLGGDAPEETARP"
     gene            35112..>35329
                     /locus_tag="AC16QF_17505"
     CDS             35112..>35329
                     /locus_tag="AC16QF_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017845109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HIR6450408.1"
                     /translation="MSLMDEGSIAVLPAAGQRFRNRNTEYPFRQDSDFYYLTGFAEPD
                     AVLVLAPGRAHGQVILFCREREARAERY"
BASE COUNT         6188 a        11112 c        11790 g         6239 t
ORIGIN      
        1 gcgtgtcggc ggagcggatc cgccagatcg agcagaatgc gatcaagaag ctgaaagccg
       61 cgtgtatgag cgacgccgcc tgatccgctg ggcatcgggg gtacgatgcc ggccaacgtt
      121 cctttagccg cgggagccct cttcggcctg accggcgtag tgatgggcgc ccttggcgcc
      181 cacgggcttg ccgggcaact gtctgaaagc ggcgtcgata tctgggacac cgccgtgctc
      241 taccatctca cgcacgccct agccctcgtc gccgtgggcg tgcgcttggg ccagaaggtg
      301 ccgccgccgt ggccggtcgt tctggccggc tgggggtttg gtgtcggcat cctgctattc
      361 tccggcagcc tttacgtgct cgctctgggc gggccacccg tcttcggccc tctgacgccc
      421 ctgggtggcc tcgcgctctg tgccggctgg gtcgccctgc tcgccgccgc ccgccgctga
      481 gcgccgctcg ggacacgcat tggaagaaag gacttacctg cgccgccgcg ggcggatgac
      541 tcggggtcag gctcgggccc tcgaccgagc gggcgacacg atgatcgtcg actcgacatc
      601 gcccgactgg cctgcggtgt tcggccggca agccccgctg ggggtggagg tcggcttcgg
      661 gatgggacat gccctgctgg actgggcggc cgagtgcccg gattggaacc tggtggggat
      721 cgaggtctat cagccgggga taggcgctct gctgctgggc ctggactccc agggctcgac
      781 caacgttcgc gtgctggaag cggacgcgag gcaggcgctg gctgcgggct ttgagcccga
      841 ctcaatcgac gaggtgcgga tactctttcc ggatccctgg cccaagaaac gtcaccacaa
      901 gcgccggttg gtgcagcccg atttcgtccg tctccttgcg tcgagaatgc ggcctggggc
      961 tcggctcggc ttggctaccg actggcagga ctatgcccag cacatgctga ccgttctcga
     1021 tgcggagccg ttgctgcaga accgatcggg gcagggttcc tatgccgagc gcccttcggg
     1081 tcgcccgacc acgcgcttcg aggagcgtgg ccggcggctc gggcatcagg tgtgggatct
     1141 tggctacgaa agaaagcgcg agaccaccga gtccaggtag cgatagccct cgggggtggt
     1201 tgcgaggcga tggcggtgaa cgaggccttc atccactagc tcgccccaga ccggatcgag
     1261 agcctccagg gcaagccccg tgcgctgggt gaactggtcc agggggacac catcgcggag
     1321 gcgaagcgcg ttgagcatga actcgaaggg cagagcttcg gcttcaacga tctgccgctg
     1381 ctggctttcg gggtcctgca ggtaaaggcg cggctggctg ggctttaccg tgcgggccac
     1441 ccgaccaccg cttgtcagct tgccgtgggc gcccgctccg atcccgacgt agtcgccgaa
     1501 cgaccagtag ttcacgttgt gacgcgatcg ccgacctggc ctggcgtacg ctgagacctc
     1561 gtagcgctcg aagcctgcct gctcgagccg atcacgaccc cgttgctccg tctcgtcgat
     1621 tcggtcctcc gggggaatcg gggggggtcg ccttgcgaac tcggtgcgcg gctcgatggt
     1681 gagctgatac cagctgatgt gatccggttc cagggccagg gcggcctcga ggtcctgaac
     1741 tgcctggtcc ggagtctggt gcgggagacc gtacatcagg tcgatgttga tgttctcgaa
     1801 cccggccgat ctggcgcgcg cggcgcaatc cagggtttcg gcggcatgat ggatgcgccc
     1861 aagcctctcg agcgcctcgt cgtcgaacga ctgcgctccg atggacaggc ggttgatgcc
     1921 cgcctgccga taggcgcgca ggtctgaatg ctcggtggtt ccggggttgg cttccatggt
     1981 cacctcggcg tcctccgtca gctgggaggc gacctcctcg aggacggcgg cgaacgccgg
     2041 ggcatggaac aggctgggcg tcccaccgcc gaagaagacg ctgccgatgg gcacgtcgcg
     2101 cggcaggttc tttcgcaggt cggcaagcag cgcgctcacg tagccggcct cgtcgataga
     2161 ccctcgaacg gggtgagaat tgaaatcgca gtacgggcac ttgcgcacgc accagggaaa
     2221 gtgggcgtac aacccgaccg gccccatcaa acgactccgg ccagcgcggc gcacagcgcg
     2281 cgcatggccc gaccgcggtg gctgatctcg ttcttcacgg acgggtccag ctcggcggcc
     2341 gtgcaatccg tctcgtcgat gaagaaatgc ggatcgtaac cgaacccgcc cgtgcccctg
     2401 ggatcggcaa cgatgcgacc gctccaggcg gccgtggcga cgacgggtac agggtcgtcg
     2461 ggtgcgcgca tgaagaccac ggcgcagtga aagcttgcgc ggcggtcgtc catgtcggcc
     2521 atggcggcga ccagcttggc gttgttctcc gcatctgtgg catcttcgcc cgcatagcgg
     2581 gcggagcgca cgccgggcgc cccgcccagc gccggcacgg cgagtccgga gtcgtcggcg
     2641 atcgccggca tgcctgccgc ggcgctgacg tggcgggctt tctccagcgc gttctcgatg
     2701 aacgtggccc gtgtttccgg tgcgctcgca atgccgaggt cgcgctggct gaccgcatcc
     2761 aaccccagcg gggcgatcag gagccgcaac tcggcaagct tgccggggtt gttgctggcg
     2821 agaacgatgc tcaccgcggg atcacgtgtc ttcccagtac agcttctgct ggaacttgag
     2881 ctgcatgggc ggcgcgtcgg gagcgctaag ggaaatctcg aaccggagca cctcctcgtg
     2941 ggtgtgcttg acggccgcca gatagtagac ggcctggccc tcggtgacct cccggaacgc
     3001 cagcggctgc agctgaccca acagattctt cacggtgcct tcgatggaga ccttctgggg
     3061 agcgcccgtc gggtcgagca cggaaatatt gaccagggcg cggtcgcggc cccgagtgat
     3121 cccgtattgc gcggcgatgg ccggtttcag gaagccggtc ggcagcacca cgtagtgcac
     3181 gtcccagcca ccgaaggact gccgctgctc ggcgtgtccc ggcgtccccg cccacgacag
     3241 gatgagcgcg cagatccagc cagcgcccgt cttcaccatc aggcctctcc cgaattcggt
     3301 ctgcccagcc tatagaaggc gatgtgaccg aagaagttgg gaaatcgctg catcagccgt
     3361 cttgcgagat gccgctcgtc gacgacgaag cgatcgatga ccggtaggcc tttgctgtgg
     3421 cacagccgtt cgaaatcctt gaaggtgcac aggtggatgt tgggcgtgtc gtaccaggcg
     3481 tgaggcaggt gctcggagac gggcatacgg ccgcgcagcg ccagatacgc gcggcaacgc
     3541 cagtagccga aattcggaaa cgtgacgatg cactcgccgc cgatccggag catgtcgtcg
     3601 agcatgcggt cggggttctg caccgcttgc agcgtctcgg tcatcaccac caggtcgaag
     3661 ctgtcatccg gaaaattgtc gagcccctca tcgagatcct gctcgatgac gttgacgccg
     3721 ttgtgcaggc aggtggtgat gttgtcggga tcgatttcca gcccgtagcc gttgacctgc
     3781 tttgaagtct gaagataagc gagcagctcg ccctcgccgc aaccgagatc gagcacccgg
     3841 gctccaggtt tgatcacctc cgcgatgatc tccaggtcag ggcgcatcga cggcctccgt
     3901 ggcgatgcgg cccagatagg cccgaagcac gtcgaagtat cgcggcacgt tcaggagaaa
     3961 cgagtcgtgg ccgtgctccg actcgatgat ggcgctggcc acgttcttgc ccgccgcgat
     4021 gagcgcgtcc accagctcct tcgagcgcgc cacggagaag cgccagtccg tggtgaacga
     4081 gatgacgcag aagcggcagg tggcggatgc cagcgctttg accaggtcgt cgtcgtgctc
     4141 ccgggccggg tcgaagtagt ccagtgccct ggtcatcagc acgtagctgt tggcgtcgaa
     4201 gcgctgagag aacgagcggc cctggtggtg cagatagctc tccacctgaa actccacgtc
     4261 ctgggcggtt tcgatatccc ctgacttcag ctgccggccg aagcgctggc gcatgccatc
     4321 gtcggacaga taggtgacgt gacctaccat gcgcgcgagc atgaggccca ggtccgggtt
     4381 gacgcccagc tgccggtagt tgccgccgtg aaactccgga tccgagatga tggcctgccg
     4441 tgcgatctcg ttgaaagcga tgttctgagc ggacagcctg gcggcgcagg cgatcagcac
     4501 ggcggcgtga aggcgatcgg gatagtcggt tgcccagcgc atggcctgca tgccccccag
     4561 gctgccaccg acgacggcgg cgaagcgatc gatgcccagg tggtcggcga gcagggcctg
     4621 agtatctacc cagtcttcaa cggtgacgac gggaaagttt ggcccgaacg gttcacccgt
     4681 gcgggggtcg accgtggtcg gccccgtgct gccgtggcag ccgccgagat tgttggggca
     4741 gacgacgaag aaccggtctg tgtcgattgg cttgccgggc ccgatgcagc tttcccacca
     4801 cccgggtcgt ttgtcgtcct cggagtggta gccggcagcg tgatggctcc cggagagagc
     4861 atggcagacg agaatcgcgt tgctccgcgc agcgttgagc cgtccgtagg tctcgtagac
     4921 gagctcgaac ccctcgagcg tctcaccgca gcgaagcgaa aagccggagg gatgcgcgaa
     4981 gacctgcggg ctgaccaggc ccacggaatc ggatggcaag ctgctcgaca ctgagtaggc
     5041 cacgaagaac gggacggcga gtctaaagca agggcttcaa ggccatcaac cgggtcggtc
     5101 ggggcatccg gccggggcgc agccgattag ctcagatctg cagcagcacc cgttcgatga
     5161 cgccgaggat caggaaaacg aggatcggcg agaagtcgag accgcccatg ggtggcagga
     5221 gcttgcgggc cggtgccagg acgggctctg tgagctgctg gagcagggcc agcgccgggt
     5281 ggtagttacc cggagcgagg aagctcgcga tgatcacgat cagaatcgac cacttgatga
     5341 tgaacagccc cagcagaatc atctgcagca cccctgaggt tgcgatctgc acagcggttc
     5401 ccgtgggccg atcggcgatg gcccccaggg cgaggatcat cacgacctcc acgatgagcg
     5461 ctgccaggag ggcggcgaaa tccagatttc ggtatccggg cagcaccatc cggaccggct
     5521 tcaacaccac gtcggtggct ttgacgatcc cttgagaaat ggggttgtag aagtcggccc
     5581 ggcatgcctg cagcaggaaa cgaaccacga acaggaaggc aatgacctgc agaatgaagc
     5641 tggttgcgta atataagacc tctgccatgc tcgttgccta ttcccctgat tacgacgttt
     5701 agcgaccgaa ctcgatggcg agctcgcggg atcgttccgc ggcggccgct acagcggcgt
     5761 tgatgatacc ccggcagtcg gcggcatcca gtatttccag cgccgcctgg gtcgtcccgc
     5821 ccggagaggt cacatttgcc ctgagctgcg cgggtggggg cccttcgaga gccatcagcg
     5881 cggcgccgcg ggcggtttcc agcgtcaggg tgacggcggt ctcggcgctg agccccaggg
     5941 cctggccggc ttcgatcatc gcttccatca ggtaaaagaa gtaggcgggc ccgctgccgg
     6001 agacggcggt caccgcgtcg agctgatctt ccaaggatac ccagagggtc ctgcccgcgg
     6061 tggccagcac gctttctgcg agctcccttt gagccggcga gacgcgctcg ttggcgcaca
     6121 gggcggtgac tccggcgccg agaagggccg gcgtgttcgg catgcagcgg acgatgggct
     6181 gctcgctgct ggtccaaccg gcaaggctcg acatgggaac gccggcggcg atggagacca
     6241 ggagctgatc cgtgctcagc gccgtcagcc ccccggctac cgaggccatg acctgcggct
     6301 tgaccgcgag gatcaccacg tccgcaccgc gtacctgggc attgttgtcg tcggaggttg
     6361 ccacgccgag ctcggcgatc ttgcccagct gctcgggtgc ggggtcgctg gcgcgaatgg
     6421 tcgccgcatc gtgaccgctg gcgatcaggc ctttgatcag gctggtcgcc atgttgccgg
     6481 cgccaatgaa agtaatgtga ctgctcaacg gatcggaact ccgggtagtg gctcgttcgg
     6541 gttagccctc gcgcgcgccg aagatggcgg tgccgatgcg cacgtgggtg gcgccggcca
     6601 ttatggcctg ctcgtagtcg ttgctcatcc ccatggacag ggtatcccag cgaccggcgg
     6661 cgaggggcgc gagcccggcg aacagcttcg ccatgcgcgc aaagctctcc ggggcatccg
     6721 cgttcgggtc gaggatggtc atgaggccgc gcacgcagat ccgctcgaga tcaacgagct
     6781 ccttcagcag atcggcgacc gcggctgggt cgacgccggc cttgccggga tcggcgtcta
     6841 tgttgacctg cagacagacc tgcagggttt tgtcgtcagg acactgctcg ttgagccggc
     6901 gggcgatctt gggtcgatcc accgtatgca cccagtcgaa atgccgcgcc acctggcggg
     6961 tcttgttcga ctgcagcgcg ccgatgaagt gccaggtcag gggcagatgc ccgagcgcgg
     7021 cgatcttcgg cagcgcttcc tgcagatagt tttcgccgaa gtccttcaag ccggcgccgt
     7081 gggcctcttc gatggcacgg gccggttgcc gcttggagac tgcgatcagg cagacctcgt
     7141 cggggtcccg ctgtgcggct cgcgccgcag agacaacaca ggaacgcacg ctggcgacgt
     7201 tgtgagagat cgagtgcaat ttcgttttgc caactgcgtc gcagatttcg catgtctccg
     7261 gcagagcgag gagggattca tggttaaagc ccaggtcaga taatactata cagcccggat
     7321 tcgcagcggc tttcgttgcc atttcggcga gtttgaagcc caagcgagta gacaaggatc
     7381 gctgatggac attaccgaac tcctcgcttt cagcgccaag cagggcgcat cggacctcca
     7441 cctgtccgcg gggcttccgc cgatgattcg tgttgacggt gacgttcgcc ggatcaacct
     7501 gccgccgctc gagcaccagc aggtgcattc gctgatctac gagatcatga acgacaagca
     7561 gcgcaaggac ttcgaagagt tcctcgaaac cgacttctcc gtggaagttc ccggcgttgc
     7621 gagattccgt gtcaacgcgt tcaaccagaa tcgtggcgcg ggcgcggtgt tccggaccat
     7681 cccgtccaag gtgctcacca tggaagacct ggacatgggg caggttttcc gcgacatctg
     7741 cctcgttccc aggggcctgg tgctcgtcac gggacctacg ggatccggta aaagcacgtc
     7801 gctcgccgcc atggtcgact tcatcaacga ccatcgctac gaccacatcc tcaccatcga
     7861 agatcccatc gaattcgttc acgagagcaa gaagtgcctg gtgaaccagc gcgaagtgca
     7921 tcgagatact cacgggttca acgaagccct gcgctcggcg ctgcgggagg acccggacat
     7981 catcctcgtc ggcgagatgc gcgacttgga gacgatccgg ctagcgctca ccgccgccga
     8041 aacgggccac ctcgtgttcg gcacgctgca cacgacgtcg gcggccaaga ccatcgaccg
     8101 ggtcatcgac gtgtttcccg cagaagagaa gtccatggtt cggtccatgc tctcggagtc
     8161 gctgcagtcg gtgatctcgc aaacgctcct caagcgggtc aacggcgggc gcgtggccgc
     8221 tcacgagatc atgatcggca cggcggccat ccggaacctg attcgcgagg acaaggtcgc
     8281 gcagatgtac tcggccatcc agaccggcag ctccgtaggc atgcagacgc tggatcagtg
     8341 cctgctcgat ctcgttcaga agcgcacgat cacccgcgac tcggctcgcg agaaagccaa
     8401 gaacccggat gacttctaac ggcggaggtt agcgccatgc aattcgaaaa gctgttgaaa
     8461 ctggtggtgg aaaaaggcgc gtcggacctt ttcatcaccg ccggcaagcc gcccatgctc
     8521 aaggtcaacg ggaagctgtt cgccgctgga accaccaacc tgtctgccga tcagtcccgg
     8581 gatgtcgtgc acagcatcat ggacgaggac cagaagaagc agtttcacga ccatcacgag
     8641 tgcaacttcg ccattcattc cgaagggctg ggtcgttttc gggtgagcgc gttccagcag
     8701 cgcaacctgg caggcatggt gctgcggcgc atcgaggtca acatccctct ggtctccgat
     8761 ctgggcctgc cgcccatcct ggaggacctg tcgatgacgc ggcgcggcct catcattttc
     8821 gtgggggcga ccggcaccgg taagtccacg tcgctggccg ccatgatcgg tcatcgcaac
     8881 gagcactcct cgggtcacat catcagcatc gaggacccca tcgaattcgt gcaccagcac
     8941 aggggctgcg tcgtgaccca gcgcgaggtg ggcgtagaca cggaaagctt cgaggtcgcc
     9001 ctcaagaaca cgctgcgcca ggcgccggac gtcatcatga ttggtgaggt gcggacccgg
     9061 gaaaccatgg agcacgcggt ggcctttgcg gaaaccggtc acctctgcct ctgcaccctc
     9121 cacgccaaca acgccaacca ggcgctggac aggatcattc acttcttccc cagcgaccgt
     9181 cactcccagg tgtggatgga tctttctctc aacctgcgcg ccatggtggc ccagcagctc
     9241 gtccccacgg tggacggaca gggacggcgg gcagcgatcg agatactgat caacacgccg
     9301 ctgatggccg accacatccg taaaggtgag gtgcatctgc tcaaagagct gatgtccaaa
     9361 tccaccgagc acggcatgca gacgttcgac caggcgctct atcagctcta cagcgagggc
     9421 gagatcagcc acgaggctgc gctgcagcat gcggactcgc cgaacgacct gaggctgatg
     9481 atcaagctgg gttccgatgc aaacccggac ctgatgggtc aggtcgaagg gttgaccatc
     9541 gaagaagacg agccggcggg taccttcctg cgctgagagc cgctcttccc cagcggttca
     9601 gccatcggcc gccaaccagg attccgcgat catcgttgct gcggcctcgt gaggccgatc
     9661 gctgccggat tcgcgcgccg caaacgaagt cagccgctcg tcgaccagct cgatgggaac
     9721 gccgaaccgg cggcccattc gctcggcgaa cccgcgagcc ttgagcgaca tttcgctctc
     9781 gctgtcgtcc atgttaaggg gtaggcccac cagcagcacc gccggctgcc actcggtaat
     9841 cagccgttcc acgtccgacc agtccggcgt tccgtccctg gccgcgatgg tgatcagcgg
     9901 gctggccgtt cgggtaaccg attggccaac ggccacgccg atgtgcttca gcccgaagtc
     9961 gaacgccagc acgtgaccct gcttcacgcg gctgtcaggc gtggcccgcc tgtgaggaca
    10021 tcagccggaa atcgatgccc agcgcggcag ccgcctggtt cacgcgttct gcgaaaggcg
    10081 tgcgaaagag gacgtcggtg tttccagggc aggtgagcca ggcgttttcc agcagctcct
    10141 gctccagctg gcccccgccc cagccggcgt atcccatggc gaccaggaag tccgcgggcc
    10201 cctcgccggc cgcaatggcc tcgagcacct cgcgggtgga gttgagcatc agcccgtccc
    10261 ccagagagag cgaagagtca aaggctcggt cgtcgctgtg cagcacgaat ccgcgctcgc
    10321 tcgccacggg ccccccttcc atcacgggta tgtccgccga cacggcgctg gagtctatgc
    10381 cgagatcggc cagcagcgcc tgcagcgtaa cttccgtcgg gcgattgacc atcaggccca
    10441 tggcgccgtc ggcgctgtgc tcgcatacgt aggtgaccgt gtccccgaaa taggtgccag
    10501 ccaggccggg catggcgagc aggaactggt ttttcaggct ggtatcagct gccaaggcgg
    10561 gctccggagc gtgagaattt ccaggtgcga atgatctcca actgatcgta cttcttgcgc
    10621 atctccaccg gaaactgctg gtagggcgca gccagacgga cgattcgaag cgcggcgtcg
    10681 tcgagggatt tgtgcccgga agactccaga atccggacgt ccttgagcgt gccgtcgtag
    10741 ctcagcacca cgagcatccg cagattgccg tacacccggc tgtcgggata gttggcgttg
    10801 ccgatgcgct cgaccttctg acgccacatg ttcaggtagg cggcttcgtc tgctgacttg
    10861 gcggacacgc tggtgagccg cttcaccctt ggctgcttcg ccttggcctg ctggtccgcg
    10921 gcgatcctgg cttccaggct cgcgatctcc ttcgccagat cgtcgaagcg aatgtcggtt
    10981 gccggctgcg gattgacgtt ttcctccacc ggcgtctctt cttcaatggg cgccacgtcg
    11041 tctacctcgg agatggtcgt caccagctcc cgagagtcaa gctggttctg ctcgatggtc
    11101 ggcaaggggt tggatatcgc gtcggcgaac tcgttggcga aaaactccgc ttcctggtcc
    11161 gtcgtggtct ccagcacttc gctctcggtg ccgctgccga gctggttggt ctgagcgatg
    11221 aagtcggcat cctcgggtgc ttcgaggtcg ctgtgctggg caaacgtcac ctcgaccgta
    11281 gggctttcga gcaggtcgag gtctgccgtg aagctgacgc cgagaatgat ggcggcgtgc
    11341 agcgagacgg ccagaaacag ggtgaagccc agacggtctc gtggagtgat ggcggcggcg
    11401 ctcatgtttg cagggtgtcg acggggcgtt caggcctgag catctagctt ggcagatatc
    11461 gtgtccatga gcataccgcc gatattcagc gaaaactgtg catcgagttc ccgtatgcag
    11521 gtcgggcagg tgacgtttac ctccgtgaga tagtcgccga tgacgtccat gcccacgaac
    11581 agcaggcccc gccgacgcag ctcgggaccg acctgagcgg cgatccatcg gtcgcgctcg
    11641 gtgagctccc gtccctcgcc cgtgccgccc gccgccaggt tgcctctcgc ctcgccggcc
    11701 atggggattc tggccagggc gtaggggatc ggctcgccat cgatgagcag aacgcgcttg
    11761 tccccgctgg caatctccgg aatgaacttc tggcccatga tctgggcggt gccgcctttc
    11821 gtgagcgtct cgatgatgac gcccaggttc tcgtcgccct ccatgatgcg gaacacgccc
    11881 tggcccccca tgccgtcaag gggcttgaac accacgttgc cgtgagtccg ttgaaactcg
    11941 cgcagcgccg cttcggaccg ggtcacgacc agtggggggc aacattcctg gaaggcggtg
    12001 gcaaagaact tctcgttgca gtcgcgcagc ccttggggtt tgttgaccac cagcacgccc
    12061 aacgcttcgg cccgttcgag gaggtaggtg gagtagatgt actccatgtc gaacggcggg
    12121 tctttgcgca tcatgatcac gtccagctcg gcgagcgggg taagggccgc ttcgctgagc
    12181 tgataccagc gatcgccggc ctcggccgga tcgagggaat cggcaaacgg cccgacgagc
    12241 tccagggatc ggagcatccc gcagggcgcg ccctcatgga gcagcaggtc cccctgctcg
    12301 agatagccta cctgccaccc cttgagctgg gctgcgcgga tcattgccag cgtgctgtcc
    12361 ttcttcagcg acagggtggg cagagggtcc atcacgaagg cgattttgag actcatgggt
    12421 tctgtggtgc tccgggcgag ccgggacaga agttgctccg gctggggcgg taactagctt
    12481 ggcggtcgta gcgtaacccc aaaaatgaga acgaattccc tttcttatcc aacgcttata
    12541 gatcgtttcg ggacaatcat gatacaacgg gagcgttcgg taggtacgtg aaaatcctgc
    12601 cgacagcctt gatgacgcca tactcaatgg acggtgacac gacggtcaag tcggaagtag
    12661 aaggaccaga tatggaacag gaactcaaag gcgttaaggt gctggtaatc gacgacagta
    12721 agaccattcg tcgaagcgcc gagaatctgc tgaccaagga agggtatgac gtcatcaccg
    12781 ccacggacgg ctttgacgcg ctcgcgaaaa tcgtcgattc acggccggga atcatcttcg
    12841 tcgacgtcat gatgcctcgt cttgacggtt accagacctg cgcgctcatc aagaacaaca
    12901 gcgaattcaa gactaccccc gtagtcatgc tctcgagcaa ggacggcctc ttcgacaaag
    12961 ccaaaggccg gatcgtgggt tcggatcagt acatcaccaa gcccttcagc aaggacgagc
    13021 tgctcggcac gatccggaac ctcgtccatt aggaagcagg cacaggacgc gcagaagatg
    13081 gcaaggattc tcgtagtcga cgactctccc tcggagacgc accagctggc caacgcgctt
    13141 tcgcgtcatg gtcaccaggt gctgacggct gcctccggca gcgacgggat cagtctggcc
    13201 aacgaagagc agcctgacat cattctgatg gacgtcgtga tgcctggtat caacggcttt
    13261 caggcaacgc ggcagctcac gagaagccag ggtaccagcc acattccggt gatcatcgtc
    13321 tcctccaaga gccaggatgc ggaccgggtc tggggcaaac gccagggcgc gcaaggctac
    13381 ctgaccaaac ccgtggacga tcgcacgctg atcagcacgg tcaacgaggc tttgagcgcc
    13441 taatccttgt catgaacggt caagcgcaaa acatactcac ggacatccgg gccaccacca
    13501 tcgccaacgc ggcgcctctt ccttcccggg agaacgagca tctggagtgg cagggtgtcg
    13561 gttatcagct gggcggtgtc cgcctggttt ctccaatggg cgagatcagc gagattctcg
    13621 ccgtgccgaa gttcgcgacg ctgcccggcg tcaaaccctg gatgctcggg gtggccaacg
    13681 tccgcggcag gctcataccg atcatcgacc tccacaattt cctcgacatg cgcacgacca
    13741 cgcccatggt tcagtggcgc gtgctggtag tggaagacga ggaactgatc gcaggcttcg
    13801 tggtggagca gtcgctcggc attcagcatt ttctagaaga cagttttgaa gaaagcacgg
    13861 cggaggaagt agcggcactg aagccctact tgcgcggtgc ttttcatcag ggagggtgcg
    13921 ttttttacga ggcgcatctt aagtcgatat tgagggacga gaagttcttc gatgttgcgg
    13981 acaagactaa ctaatcaggt tgtacggaaa tgaaaacagg aaagttgtct ccgttgctag
    14041 tggcgttgat gtgcttgctg gtcggcacga ttgcagggat ggcctacgtt gtcgtcactg
    14101 atgaagcatc catcatcaac aatacgggtc ttctgttcgc cgttgccggt atcgtgctcg
    14161 tcgccgccat cgcggcgatg atggtcagcc agacgcgccg gcagctcgtt gagcaggcgt
    14221 cacagaacga acgcaatcag gccgcgattc ttcagctgct cgacgagatc gccgatctcg
    14281 gtgagggcga tctgaggatt caggcctcgg tgacggagaa cttcaccggc gcgatagccg
    14341 attcgatcaa cttcgccatc gaccagatgc gcgggctcgt atccaacatc aacaacctgt
    14401 cgcgtcaggt tgctaaagcc gcgggcgaca gccaggagac ggcatccagc ctcgctggcg
    14461 cagccgagag ccaggcacga gagattgccg ccgcgtccca ggcgatcaac gagatggccg
    14521 tctccatcga tcacgtctcc tcgaacgccg ccgagtctgc atcggttgca gagcgggccg
    14581 tgggcatcgc caacaaagga tcggacgtgg tccaggacac catccacggc atggacgtga
    14641 ttcggggcca gatccaggaa acgtcgaagc ggatcaagcg cctcggcgag tccagccagg
    14701 agatcggcga catcgtatct ctcatcaacg acatcgcgga ccagacgaac attctctccc
    14761 tcaacgccgc cattcaggcc tccatggccg gtgatgccgg tcgaggattt gccgttgtgg
    14821 ccgacgaggt gcagcggctg gctgaacgat ccagcgcggc aaccaagcag atcgcggctc
    14881 tggtgaagac cattcagacg gacaccaacg gggccgtaat ctccatggag cagaccaccg
    14941 cagaggtcgt caacggtgcc aagcgcgccc aggacgccgg ggtggctctc gaggagatcg
    15001 aaagcgtatc cacgtcgctg gccgagctga tccagaacat cagcaacgcg gcacgccagc
    15061 aggctgcctc tgcgggccac gtttccaata caatgaacgt cattcaagag attaccgcgc
    15121 agaccgttga cagcaccgag caaacggcga cttcgatcgg taacctggcc gccatggcca
    15181 acgagatgcg cgaatcggtc gaaggcttca agctgccggc ccagtaccaa agccaggatt
    15241 aagacctcgg tatggcgaca agccgggaaa attttgccct cgactgggta aagggcgcgc
    15301 tgcaggagtc tctggacgaa gcgatcgcgg cgctagactc gttcgctgaa gccggtcacg
    15361 gcgaagcgca gatgcgcgaa tgcatgacgg ctctgcacca ggtgcacggc gctctggtga
    15421 tgctggagct gaagggcacc acgctgctgg ccgagcacct ggagcggctg gcccagcacc
    15481 tgatgatcgg tgcggtcggc gatagcgttg gggccggtca gtgcctcatg caggggcttc
    15541 tcgagctgcc cgggtatctc gacgaggtgc agcgcgggct ctccgacgcg atcgaaccca
    15601 tgatcccgct gatcaacgag gtccgcggtc atctggacga gcggccgttg gaaggcgcga
    15661 tggtcaccga cctgggcgtc gccagcgaag cggccataga gcgcttcgat gggatcgacg
    15721 gcgtagagaa ggcggggcgg atacgggcgg cttaccagca ggtcctgctc tgcatcctgc
    15781 ggggagacga tcgcgaacgg gcggtcatca ccctgcgaaa gattgccgag gtgctgtcca
    15841 gggtatgcct gggcactcct catgagaccc agtggcgcgc gctcggcgaa tttgccgcga
    15901 gcctggccac ggacaccggc cccatcgacc cggacgcggc aaagctgctg cgccggatcg
    15961 actcggagat ccgcacgctg tccagagacg ggctcggagt tgccggcagc ccggtttctc
    16021 tagacctgat caagcagctt ctggatgcgt gtcgccagcg ggggtatcgc agcgaagcga
    16081 tcaccgacat tagcaatgcg ctggcgggcc gcggagacgg cggcgggctg gccatgaacg
    16141 gcttaaaagc cctggtgagc gcaagcttgg tggtgcgcga agagctcgcg ctggtcaaag
    16201 accagctgga cctgttcgcg cgcgcgcgtg gtcgtgccag cgttgacctg aactcgctct
    16261 tggcgcccct caagcagatc cgcagcacgc tgacgctgct cggtttcgca agtgctcacg
    16321 agatcgtcac gcagcagctc gaagcgctcg agcagctggc gtcggccgaa gccgtcgacc
    16381 cccaggacct cttcagcgtc gccagcgcgc tcgtacgtgt agacgagagc atggcgagct
    16441 tcaccgaggg cgatcaggag gtcgaaaaga tcaccaacga cgcccagcgc accgtcaccg
    16501 tggaggtgcg taacggcctc gagccggtca gacaggccgt gctcgagtac atcgccgcgg
    16561 gttgggactc ggaacccctc gccggcgtgc cggagcagat tgccgctttg tgcggcgctc
    16621 tggagatgat tccgctgcgg cgcgcggccc ggattctgag cagctgtggc gcatacctgc
    16681 gacgagacct gctggaggcg ggtgccaccc cgggcatgga tgccatggaa accctggcag
    16741 acgccatcac ggccgtcgac tactacctcc agcgcacggc tgaggacatt actggcggga
    16801 tcgaggacat tctagacgtc gcggacgcaa gcctcgagaa gctcgggtat gcgggcatcg
    16861 tcgacgagcg cccggccgcg cagccgggcg aagcgtttca ggccgagacc gccggcgtcg
    16921 aagagaccgc ggaggaaggc ttcgactggg agggctttgt ctcagaggct gccgacctgc
    16981 gcgaagagcc tgcctcgtcg gatgctcagg atcaggaagc gcccgaatcc gagccggagc
    17041 tcatcgactt cgagcccgtt gccctcgagg gcggctcgga cgacgagccg gaagcagcgc
    17101 aggctgcttc cgccgacgcc gagccggaag cagagccgga ggacgcctcc gctgtggaag
    17161 agactccggt ggccgccagc gcgccgtcac ccgcgctgcc gccgctgtcg ccggccatcg
    17221 ccgaaaagac cagccggctg gccgacgcgt tcgaatccga cgaggaaatc gtcgagatat
    17281 tcgtggagga agtcgacgaa gttctggaga ccatcaacgg gtgtctgcct cgctggggcg
    17341 agcatctgag cgacgaggac tcgcttaccg agctgcgtcg cgcgtttcac acgctcaagg
    17401 gcagcggccg catggtcggc gccaacatca tcggcgagct ggcctggtcg gtggagaaca
    17461 tgctcaaccg gctcattgac ggcaccttcg agcccaccgg acagtttgcg ctggtcgttg
    17521 aagaagcaac gtcgttgatg ccgtatctgc gcgatgcgtt cgaagcccag cgggcccccg
    17581 atctcgacgc tatgggccga atcatggaga aggccgacgt gctggcgtcg ggcggctccc
    17641 tggaggagca ggcgccggtc gcggaggaag agccggccgt ggaagctgcg gaagcaggcg
    17701 agggcttcga ggcgcttggc ctgttcgtat cggaatccga ggagcatctg gccgtgcttg
    17761 ccctctgcga cgacggagaa gatttcgttc tcgacgagga tgcctacaga gcgctgcaca
    17821 ccctggctgg cagtgcgggc atggcgggca ttgaggtggt gagcgagctg gcccgtccta
    17881 cctatgaggt ggctctcgcc gcccggtctg aacaggaggt cacgcgagct tcaggtgcgg
    17941 ttgccgagtt ccttcgggcc gcgacgacgg cgctggcctg catgatagag acgttgagag
    18001 ccggctcgga gcccgaggaa gagctgcagc tcatcgcaga tgccgatcgg ctgctggtgg
    18061 agatacagga gaccggagag agcaagctcg acgagctcat gaagctgccg gccctggcag
    18121 tcgtcatgga tgcagaggag tttctgggcg gttgggctcg cggcgtcatg gacctcgagt
    18181 tcgccggtaa gctgatcggg gctctgggcg agatcgctga cctcgcgcga gcgactgagc
    18241 gctcagcggt cgccgatctg gcagccgcgc tgcgagccac tcacgaggag ctcgagcagg
    18301 agacgatcag cccggagagc catgtggctc tatcgcgcgc ccacgagacc ctgctccaac
    18361 agttcgacgc catggccgcc cagcagacgg ttgccctggc taccgcggag atcgcatcgc
    18421 tcacgtcact gcgtgaggcg gtcacggaac cgtcctcggt ggaagcgttc ggaaccggag
    18481 cgggctcttc tgccggcagc gactacgccg agggtgcagg atcccgcggc gcagaagatg
    18541 tcgccgcggg cgacaccggc acgccggccg agccgccggc ggaggccggc ccacgggtcg
    18601 cctccctgcc catcgcttcg ctgcttcctg aagaagtcga cgaggacatc atcggtatct
    18661 tcttcgagga agccgaggag atccttgaat cgctggacaa cagtattcac gaatggacgg
    18721 gtgagcccga gaacaggttg ttcctggaga acctgctgcg tggccttcac acgctcaagg
    18781 gcggggcccg tctttcgggg ttgtcggtcc tcggtgacta cacccacgag ttcgagacct
    18841 tcctcatcga ctttcagtta tccagcgcgc cgctgcccga gggcttcttc accgatctag
    18901 gcgcccgtta cgacgagctc gagatcctgg tcagcaggat tcgcaaggcc atcgaatcgg
    18961 gtgaaagggg cgaatccttc gccgaggcgc cggccagcgc gacgtcgacg cccccggcag
    19021 agcccgttgc ggagccggag cgtgtggcgg aagaagacac gacgggcgca gccgctccga
    19081 cagacgagca gccgcagccc gccgaggagg cgccagcgca ggcgagcgaa gtgcccgacc
    19141 tggcggcggc cgaagcatca gccgaggaag cagaggccga ggagcttccg gcccaggaaa
    19201 tcgcggccag cgaggagacc gacgaagccg agcccgagcc ggtcgcagag ccggagccgg
    19261 ttgctgcgcc tgcccccgtg ctgcgggtgg ccccggagcc gtccgaggaa gcgccgagcg
    19321 cgcccgatcc cacacgggcc gaccgggcca aggagatggt ccgcgtaggg gccggactgc
    19381 tcgaagagct cgtgaacctt gccggcgaaa gcagcatcgt cagggcccgc atcgagcagg
    19441 gcatgagcga cttcaccggc gccctggacg agatggaaac caccatcgag cgcgtgcgcg
    19501 agcagctgcg gcgcctcgag atcgaaaccg aagcgcagat cctgttccgt cgggacacgc
    19561 tggaaggccc gggttacgac gagttcgacc cgctcgagat ggatcgatac tcgcagcttc
    19621 agcagctctc ccgagggctg tcggagtccg catccgacat gctcgatctg aaagacaccc
    19681 tgctgttcaa ggcccgtgag tccgagactc tcctgctcca gcaggcgcgg atcaatacgg
    19741 aactgcagga agggctcatg cgcacgcgca tggtgccgtt cggccgcctg ttaccgcgcc
    19801 tgcgaaggat cgtgcgacag gtttcccgtg agcttggcaa ggacgtcgag ctgcacgcgc
    19861 acaacatgga aggcgagctg gaccgcaacc tcctggaacg gatggtgccg ccgctcgagc
    19921 acatgttgcg aaacgcggtg gaccacggga tcgagagcgc cgaggaacga cgccgcgccg
    19981 gcaagccgaa caccgggcgt atcgacatcc ggctgacccg cgaaggcggc gacgtggtga
    20041 tcgagatcgc cgacgacggg gccggcatca acgtcgattc cgttcgtgcc aaggccatcg
    20101 agcgagggct gatggcagaa aactcggtcc tgggcgacga agagatctgc cagttcgttc
    20161 tggctcccgg attcagcacc gccaagtcgg taacgcagat atccggtcgc ggcgtgggca
    20221 tggacgtggt gcacagcgag gtcaagcaac tcggcgggag catcaacatc gtctcgcggg
    20281 cgcgcaaggg ctcccgcttc gtcgttcgcg ttcccttcac cgtatcggta aaccgcgcgc
    20341 tcatggtgtc ggtgggagag gatctctacg ccatcccgct caacaccatc gaaggcatag
    20401 tcctgctcag cgccgaggag ctggataagg tgcatccgcc cctttccaag cccttcgagt
    20461 acgccggcgt ccgctacaac gtgcgatatc tcggcagcta tcttggccgt gaataccgcg
    20521 gcaacccgtc tcaggcggcg gtgccggtgg tgctcgtccg gtctggtgac cgggcagtgg
    20581 ccgtgcatgt ggatgcggtc cagggcagcc gggaaatcgt agtgaagagc ctgggcccgc
    20641 agttcgcggg tgtgggcggt gtgtccggtg caacgattct cggggacggc agcgttgtcg
    20701 tgatcctcga tctccttgcg ctgattcgcg cgcagaaggg cgacgggctg accgtcaccc
    20761 gtcgggtcat gcctggcgcg gcgaagacac gatgcgtcat ggtggtggac gactcggtca
    20821 cggttcgcaa ggtgacctcc cggctgctgg agcggcaggg catggacgtc ctcgtggcca
    20881 aggacggcat cgaagccatt gccatgctgc aggaacgccg cccggacgtg atgttgctcg
    20941 atatcgagat gccgcgcatg gacggcttcg aggtggctcg ccaggtgcgg cgcgacgagc
    21001 gcgtgcagga cctgccgatt gtcatgatca gctcgcgaac gggcgagaag caccaggagc
    21061 atgccgccga gctcggggtg aaccgattcc tcggcaagcc gttccaggag aacgagctgc
    21121 tcgccaccat cgactcgctg gtgaactaaa gagaagccca tggaacctgc taccgcacaa
    21181 gaactcatcg aaatgtcctg cgtgatgatc ccgctgcagg agacgaatct gctgctaccc
    21241 aacatctgcg tagcggagat tctgccctgg cgccgggtca gcagcatcga ggacgcgccg
    21301 aagtggtgtc tcgggatgct ggagtggcgt ggcgaggtca tccccgtggt tcgtttcgag
    21361 cgaatgggcg atgcgtcggt ggaagatgcg gaagagggtc gctgcctcat cgtgatgaac
    21421 cgctctcgca ccatcgaggg gctgccgttt tacgccctgg cggcggaggg tctgccgcgc
    21481 atcgtgcagc tcaccgccga ggacatgtcg aacgtcccaa ccaatcttgg tgtgtccgag
    21541 accatggccc tcagggttgg cagcgagccg gccatcatcc ccaacctgca gtatctcgag
    21601 gttcaggtgg ccaacctggt gcgaggccag gaaaccgcca agcaggctta gcgccagccc
    21661 caggactgtc ggaccgcagc gagggcggcg atgggcgcgg tttcggcccg gagcacaaga
    21721 tcccccaagc cgtagcgatc ggctcccgcc gcttgagcca gctcccgttc gtgctcggtc
    21781 caccccccct cgggtccgac catcaggacc agcgagtcga aaggcagcgc gccgggaaga
    21841 gccgctgccc ctggatcgag caggatgagc cgctggcagg ggcgttcggt caaaagcgct
    21901 tcgagccgtc ggggctcgtg cacccgcggc aggaacagcc gctcgctctg ctccgttgcg
    21961 ctcttggcga ttccccgcca gtgcatcagg cggccagccg tgcgccccgc gtcgattctg
    22021 acgttgctgc gcagggcctc gatgggccag atgtccgtga ccccgagctc ggttgctttc
    22081 tgaatcacga gatccatggc ctggcctttc agccatgcct gggccaggtg cagccggaca
    22141 gttgccggcg cggcgttgcg cagctcctcg gcgatctcga tcgagcagga gcgggggttg
    22201 ggttcggcca gccgggcagc atgctcgcgc ccgctgccgt caaagcatcg cagagcgtcc
    22261 ccggccttca ggcgcatcac ccgcgtgagg tagtgggaat ggtcaggatc gaggcgaacg
    22321 gttgcaccgg gcagcagcga cgcctccagg tagatgcgcc gactcatccg tcccggttag
    22381 ccaagcccag gttctgacag atcgcccggc tcgcgcccca tcggttgagg gtatagaaat
    22441 gcagaccggg agcgcctgcg cgcagcaggt cgtcgcacag gcggctgacg gtttcgatgc
    22501 cgaaccggcg caggccgtcg tcgtcatcgc cgaaggcttc cagatgcttg cgcagccagc
    22561 ggggcacgtc cgcgccgcag ttgtcggaga aacgaatcag gccctcgtag ctggtaatgg
    22621 gcatgattcc cgggtagatg ggtatggtca cccccgcccc cgcgcagcgg tcgacaaagt
    22681 cgtaataggc gtgcgggctg tagaagtact gcgtgatcgc gctgtcggcc ccggcgtcta
    22741 ctttgcgccg gaagaattcc aggtcggagc cgggggatct ggcgtcggga tggacctcgg
    22801 gataggcggc cacctcgata tggaagtggc tgccggaatg cttccggatc cggcccacca
    22861 gggcttgcgc gtttgcgcga aagcgcgagc gtccgatgcc cgaaggaaca tccccacgca
    22921 acgccacgat acggtcgatg cccatgccgc gatacccatc gagcatggcg aacgtggtct
    22981 cctcctcgtc ggccccgatg gacaggtgcg gcgcggtggc gtgcccgctg ctcacgagcc
    23041 gctcgaccgt ctgcttggtg ccgtcccggg tcgatccgcc ggcgccgtac gtgcaggaat
    23101 aaaaatctgg ccgcaactcg tcgagtttag ccgtcacccg ggcgaggctt gcgaaaccct
    23161 cctcgctgcg gggcgggaag aactcgaagc tgagccttgg ccgctggcct cgttcgggca
    23221 tgctgacgcg ttactccggg ttgacgttac agcgctgccg ccagtgcctc cgccttgtcg
    23281 gtgcgctccc aggtgaattc gggctcagat ctgccgaaat gaccgtatgc ggccgtgttc
    23341 cgatagatcg ggcgcttcag atccagcatg tcgatgaggc ctttcgggcg caaatcaaaa
    23401 tgctcacgta cgagttgaat gatcctctcg tcgtcgagct tgcccgtgcc gaaagtgttg
    23461 atgctgatgg acgtgggctc ggcgacgccg atggcgtagc tcacctggag ctcgcatctg
    23521 tcggccagcc ccgcggcgac gatgttcttg gccacgtagc gccccgcgta agcggcggaa
    23581 cggtcgacct tggacgggtc tttgcccgag aaggctccgc ccccatgccg cgccacgccg
    23641 ccgtaggtgt cgacgatgat cttccgcccg gtcagcccgc aatcgccgac gggaccgccg
    23701 atcacgaact gaccggtagg gttgatgaag atgctggtgt ctttcgagat ccagttgctc
    23761 ggcaccacgg gcttgatgat ctcttccatg accgcctcgg cgatggtctt ctggtccacg
    23821 tctggcgagt gctgggtgga cagcacgatg gcttcgatgg ccgcggggtt gccggcctcg
    23881 tcatagcgaa acgtgagctg gcttttggcg tcgggccgca gaaatttcag cgagctccgg
    23941 cggacctcgg cctgcttctg aacgagccgg tgggcgtagg tgatgggggc cggcatgagc
    24001 acgtcggttt cgttggttgc gtagccgaac atgaggccct gatcgccggc accctgctcg
    24061 tgctcgctgg tctcgtccac gcccatggcg atgtcggagg actgcacccc gagcgcgttc
    24121 atcaccgtgc agtcccaggg gtcgaaacct tcgtcgtggc tgccgtaacc gatgtccgag
    24181 atgacctgac gggccagccg gtcgacgtcg acccatgcat cggtgcggat ctcgccggcg
    24241 agaacgacca gattgttctt gatcagcgtc tcgcaggcga cgcgggactc tgcatcctgt
    24301 tcgagcatgg cgtcgagcac cgcatcggag atttgatccg ccatcttgtc ggggtgcccc
    24361 tcgcccaccg actccgaggt gaatatgctg tattcgttca tgtttcttcc ttccggttct
    24421 cttccaagcc ctgccaggcc ctgttgcggc aagcggcgca ttgtacagct tttctctggt
    24481 tcgactcacg gcgcggcctt tccgagccca ggcggccagt gcccgatggg gcgcgcggca
    24541 tttgcgcatg tcggtgcgag taacggacaa tagacagggt cggcaggggg ctggcaccga
    24601 tccttactct tacatcgcta gcgaaaacca aatggtaacg aggactgaca gggcgaacgc
    24661 cattcgtgcg ctcgccatgg acgcggtgca ggccgctaac tccggtcatc ccggggcgcc
    24721 catgggtctg gccgacattg cagaagtgct gtggcgcgac tttctcaagc acaacccggc
    24781 ggatccggcc tgggccaacc gcgatcggtt cgtcctttcc aacggtcacg gatccatgct
    24841 gctgtacagc gtgctgcatc tgtcggggta tgcggtcacc atggacgacc tgcgctcgtt
    24901 caggcagctt cactcgcgca cgcccggtca cccggagtac agcgaatgtc cgggtgtcga
    24961 aacgaccacc ggtccgctgg gccagggcct ggctaacgca gtgggaatgg cgctggccga
    25021 agagcggctc tcccggcgct tcaaccgcga cggtttctcg gtggtagatc accggacctg
    25081 ggtgattgtc ggtgacggct gcctcatgga ggggatctct cacgaggcgg cttcgcttgc
    25141 cggcaccctg cagctcggca agctcatctg catctacgac gacaacggca tctccatcga
    25201 tggggacgtc aaagcctggt tcacggagga cgtcccggct cgctttgagg cctatggttg
    25261 gcgcgtcata cgcgacgtgg acgggcacga cccgaattcg attcagcggg ctctggacga
    25321 cgcggtgagc cggcaggatc gaccaaccct ggtttgctgc aggacggtga tcggttttgg
    25381 cgcgcccaac aagcagggaa ctgccgacgt gcacggctcg gcgctgggcg aagaagaggt
    25441 ggcaaacgcc cgccgggagc tcggctgggc tgcgccgccc ttcgagattc cggagcccgt
    25501 ctacgcctcc tgggactgtc gggagaaagg cgccgagcag cagcggtcct gggaggcgct
    25561 gctggcggag tatcgcgccg cgcacccgga ggatgcggcg gagctcgaac gccgcgtgtc
    25621 cggcgatctg ccggaaggtt gggagggtcc gatccgcgcc ctggccgaag cgtcccagga
    25681 ggcgatggag cccctggaga cgcggaaatc gtcccagcga tgccttgccg cgctggccgg
    25741 cgccctgccg gagcttttcg gaggctccgc cgatctcacg ggctccaacg ggacccgctg
    25801 ggatggtgca gacgaagacc ggtatttgag cttcggcgtt cgcgagttcg gcatgacggc
    25861 catcaccaac ggcatggccc tacacggggg ctttcgaccg ttctccggaa cttttctggt
    25921 gttcatggag tatgcccgca acgccgtgcg gcttgcggcg ctgatgaaga tccccaacat
    25981 cttcgtgtac acccatgact ccgtggccgt cggtgaggac ggcccgaccc atcagcccat
    26041 cgagcagctc accaatcttc ggaccacgcc cggattgtcg acgtggcgcc cttgcgacac
    26101 cgtggagagt gcggttgcct gggaagcggc ggtggcgtcc cggcagcgac cgaccgcgct
    26161 gatattcacc cgccagaaga cggcgcccca gccccgttct gctcaggcgt tcgccgacat
    26221 ccgccgggga ggttatgtcc tgaaagccga ggccggcacg ctggccgccg tggtcatcgc
    26281 cacggggtcc gaggtggaga tcgccgtggc ggcggcagcg agcctcgagt cggacggcgt
    26341 cggggtgcgg gtggtctcca tgccctccac cgacgttttc ttgtcccagg accgtgccta
    26401 ccaggacgcc gtattgcccc ctgcgctgcg agcgcgggtg gcggtggagg ccgcgcatcc
    26461 tgactactgg tatcgattcg tgggcctgga cggcgccgtc gtgggcatcg accgcttcgg
    26521 tctctccgcg ccgggtggcc aggccatggc cgccctcggc atcaccgcgg acaacgtcgc
    26581 cgaggccgtc cggagcgttc tgaaggaggc ctgatctttt catgtcgcag cgaaggaaca
    26641 tgacggatct ggatctcgcg gagcggcggg tgctgatccg ggaggatctg aacgtgccga
    26701 tccgcgatgg ccgtatcacg agcgacgcgc gggtccgggc ggcgctgccg accatcgagg
    26761 ccgccatgcg cgccggcgcg agaaccctgg tgatgtctca cctcggccgc ccggtggagg
    26821 gcgagttcga cccggcgttg tccttggccc ccgtggcggg gcggctgggc gagttgctgg
    26881 gcgtggatgt cccattggtc ggcctggacg acgtgggcgt gatcgacccc ggcgccgtct
    26941 cgctgctgga gaacgttcgc tttctaaagg gcgagggtgc cgatgacgaa gccctcgcca
    27001 ggcggctggc gtcgctgtgc gacatctacg tcatggatgc gtttgccacc gcccatcgcg
    27061 cacaggcttc cacccacggc gttgcgaagt tcgccccggt ggcctgcgcc ggaccgttgc
    27121 tggccggtga gctcgaggcg ttggagcgcg ccttgagggc gcccgcccgg cccatgacgg
    27181 ccatcgtcgg gggtgcgaag gtttcgacca agctcgaagt cctggacacc ttggcgacca
    27241 ttgccgaccg catcatcgtt ggcggcggca tagccaatac cttcatcgct gccgccggtc
    27301 accccgtggg gcgctcgctc tacgaggagg acctgctgga agcggcgcgc agcattgccg
    27361 ccaaggtgga ggtgccgctg ccggtggacg tgatggtggg cgagcgcctg gacgcgggcg
    27421 cggtggcggt gctgcggggg ctcgacgagg tccgccccga cgagatgatc ctggacatcg
    27481 gtccggagac ggcccgacgt tacgcggcag agcttgggtc tgcgggcacc attctctgga
    27541 acggcccgct gggcgtcttc gagttcgacc agttcggcga gggcactcgc gtggttgccc
    27601 aggccatcgc gtccagcggt gcgttctcca tagccggcgg cggggatacc ctggcagcca
    27661 tcgacaagta cgacgtgcgc gatggggtgt cctacatctc caccggggga ggcgcgttcc
    27721 tcgagttcgt ggagggcaag accctgcccg ccgtggccgt cctcgcgagc cgggctgaag
    27781 gttaacggtc agcggtcgat ggaagtcggt ttcgacccgg acagcctcag gcaggcgctt
    27841 aagcccctcg tccccggctc tcccacggtg ctgacgccgc aggaggatgc ttacctgcgg
    27901 cactacggca ttcacttcgt cgaggagtat cccgacctgg cccatcattt cggggcggtg
    27961 gaaacggatt cgcacagcgt cgccgcccat ctctggtcgc cgcgcgatgc ccggggcacc
    28021 gccgtgatcg tgcacggcta ctacgaccac acgggccttt acgggcacct gatccgccac
    28081 ctcatcgacc gcgggcttgc cgtgctgtcg ttcgacctgc ccgggcacgg gctatccacc
    28141 ggcgcgccgg ccaccatcga aacgtttgat cactacgtcg ccgcgttcga cgcctgcatc
    28201 gcggcgctcg gcgcgcacct ccccaagcca tggcacctgc tgggccagag cacgggaggc
    28261 gcggtggcca tggagtggct cctggccaac ggcgtcgctc gggcgaccag cccgttttcg
    28321 ggcatcgtgc tcctagcacc gctggtgcgg ccgtacctgt ggcccgtcgg ccggctcttc
    28381 tacgaggtcg tgcggcgctt catcaccgag cgggagcgaa cgttctccgt caacgccgag
    28441 aatccggagt tcatcgagtt cctcaagcac cacgacccgc tgcaggcgcg cgtgctgccc
    28501 gttcagtggg tcaccgccat ggtggcttgg aagaagcgct tcgtcagccg cgcgccaagc
    28561 gatctggggc tgctggtggt gcagggcacc gccgaccgca cggtcgacca tcgctacaac
    28621 ctgaagatca tcgagcagct cttcgatgcc aaggtggtct atgtccccga ggcgcgacac
    28681 cacctggtca acgagtcggt ggtcctccgc caacagattt ttcaggcggt ggatgcacag
    28741 ctcgatgccg ccggatgagc gggggatgac atctgcgcac gggcagctga cctccgcgct
    28801 atcgccttcg caggtccgca ctgaccccga cagcctgcac gagtgggggc gggactggac
    28861 ccggactgcc gagcctgcgc ctgccgccgt cgtttttccc gagactaccg ctgacgtggt
    28921 cgccatcgtc cgcctggcca actccgaggg cctgaagctg gtgccgtcgg gtgggcgcac
    28981 gggtctttcc gggggtgcgg tggccgcgga cggagaggtg gtggtttcct tcgaccggat
    29041 gaaccgggtg ctcgagttca gcgccgcaga ccgcctgctc agctgtcagg ccggcgtcgt
    29101 cacgcggacc gtccaggact ttgcccggga gcgtgggctg tcttacccgg tggatttcgc
    29161 gtcctccggg tcgagccaga tcggcggcaa cattgcgacc aacgcggggg gcatcaaggt
    29221 gatccgctac gggctcaccc gggactggat agccgggctc agggtcgtga cgggcgccgg
    29281 cgagctgttg gactgcaatc gcggattggt caagaacgcg accggatacg atctcagaca
    29341 cctgttcgtg ggctccgagg gcacgctggg tttcatcgtg gaggcggatg tccggctggc
    29401 tcccgctccg gctccgcaaa cggtcatgct gctcggcgtg accgagttcc cgaggctgat
    29461 cgacgtgctg tccgtgctgc agagcaccgt cgtgctctcc gcgttcgagt tcttctccga
    29521 acttgcgctg cagaaggttc tctcgcatcg gaagctcagg cgacccctgg ccgagacggc
    29581 gcccttctat gcgctggtgg agttcgacac ggcggactta gagcccgcga cggcggccta
    29641 tgaggccgcg gtgggcggcg gctgggtgtg tgacggcgtc gtcagccagt ccgagggcca
    29701 ggcggcggcg ctgtggtcgc tcagggaggg catctcggag agcatcgcgc agttcacgcc
    29761 ttacaagaac gacctgagcg ttcgcgtctc ggtgttgccg gagttcctcg ctcaggtcga
    29821 cggcctggtt gcccagagct atcccggctt cgaggtgtgc tggtacgggc acatcggcga
    29881 cggcaacctc catctcaaca tcctcaagcc cggcgggctc tcgcttgcgg agttctacgc
    29941 tcgctgccac gccatcagcc ccagcctctt cgagctcgtg gcccgccacg gcggcagcgt
    30001 atctgccgag cacggcgttg gcctgctgaa acgcgacttc atcggctact cccgcagcgc
    30061 ggccgagata gagatcatgc gcgcgctcaa gggcgtgctc gatccgaatc agatcatgaa
    30121 cccgggcaag ctcctctcct gaggctcatg cgccgcaggg cgcggatccc ggctcctcag
    30181 aacgacgtca cgccggatgc gccggcgacg atgaccaggt cggcggcctg ggcggcaaac
    30241 acgccgttgc ataccacgcc gacgatgttg ttcacggccg actccatggc ggccgggtcg
    30301 gtgatggtca gctcgtacac gtcgaggatg atgttgccgt tgtcggtgac gaaaccctcg
    30361 cgcagctcgg gagcgccgcc gaggccgacg atggcgcgcg cggccagacc tctcgccatg
    30421 gggatgacct ccacgggcag agggaacgcg cccagtgtct gaacgacctt tgattcgtcg
    30481 acgatgcaaa cgaactcgtc ggcggacgcc gccacgatct tttccctggt gagcgccccg
    30541 ccgccgccct tgatgagctg ccttgccgcg ttcacctcgt cggcgccgtc cacgtaaacc
    30601 gccacggccg ccgcagcgtt cagatcgatg accggaatgc cgacagcctt gagccgggcc
    30661 gcgctcgctt cgctgctggc aacggcggca tcgaatttgt gcctgatggc gccgagcgcg
    30721 tcgatgaagt ggttggtggt ggagccggta cccacgccga cgatgctcgc gctgccgagc
    30781 ttgggctcga tgtaggccag cgctgcctct gcggcactgc gcttgagctg atcttggtcc
    30841 atacttacgt ccccgaagcc gtgctcgatg cgcggcgccc acaaaccgct gcgaccctaa
    30901 ctcatacgat tgccgtggaa aagtacgtta aaaagattct ctcctcgcgt gtgtacgaag
    30961 cggccgtgga gaccccgctg cagcccgcgc gcaatctgtc gatccgcctg ggtaaccacg
    31021 tgcggctcaa gcgggaagac atgcagcccg tcttctcgtt caagattcgc ggtgcttaca
    31081 acaggatcgt tcagctcacc gaggaggagc ggggccgggg agtcatcgcg gcgtccgccg
    31141 gcaatcacgc ccagggcgtc gcctactctg cccagcagct cggaatcaag gcgctcatag
    31201 tgatggggcg caacacgccg agcatcaagg tgaacgcggt gcgcgccctg ggcgccaagg
    31261 tcgtgctgca cggggacgcc tacgacgagg cggccgccca cgcgttgaag ctggccgagc
    31321 gcaacggcta cgtgttcgtc catccctacg acgaccccga cgtcatcgcg ggtcagggca
    31381 ccgtgggcgt ggagattctc aaccagcacg tcgccgtgcc ggacgccatc ttcgttccgg
    31441 tgggcggcgg cgggctgatc gccggtatcg ccgcctacgt caagtacctc gcgcccgata
    31501 cgcgcatcat cggtgttgag gcggaaggct cggcgtgcct ggcggcggcc atggcggccg
    31561 gtcggcgggt gaggctgccc ttcgagagcc ttgatctttt cgccgacggc gtgtctgtgg
    31621 ctcagatcgg caaggaaacc ttcaaggtgg ccaggcggtg cgtcgatgaa gtcatcaccg
    31681 ccaacacaga cgagatctgc gccgcgataa aagacctctt cgacgatacc cgctcaatcg
    31741 cggagccggc gggcgccctg gcggtggccg ggctgaagaa gttcgtcgag cgggagggcg
    31801 ttcggaacct gaacctggtt gccgtggtca gcggtgccaa cgtgaacttc gaccggctgc
    31861 ggcacatctc cgagcgggcg gaggtggggg agcaccgcga agtcgtcctg ggcgtcacga
    31921 tcccggagaa gccgggcagc ttccttaagt tctgcaagat gctcggcaac cgccctgtca
    31981 gcgagttcaa ctaccgattc gccacggacc gggaagcaca ggtctacgtc ggcctgcagg
    32041 tatcggcggc cgacgatagg gccgagctca tgaaaagcct ctccacaggc tacgtcgtgc
    32101 acgacatgac ggacaacgag gctgcaaagc tgcacgtccg acacatggtg ggcgggcgca
    32161 ccttcggcag caagccggag ctcctctatc gattcgagtt tcccgagcgg ccgggtgcgc
    32221 tgcttcgctt tctgaccggc ctggggtctc gctggaacat cacgatgttt cattatcgta
    32281 atcatggggc cgcatggggg cgcgttctgg tgggcttcga ggctcagcca cgcgaccggc
    32341 gctccctggg cgagtacctc aagcgcatcg gctaccgcta ctgggaagag acggacaacc
    32401 cggcctacca gctgtttctg cgctaggggc cgttactcgg ccagatagcg ctcgaactcc
    32461 tcgtcgaatg ccagcagggc tggatccttg agatggtcca cgtacaaccg accgtccaga
    32521 tggtcgaact cgtgctgaaa caccgtggcg aggaaccctt tcagctccag atcgagcggt
    32581 ttgccctcct ggtcgagcgc gttcacgcga acatgctggg gccgttccac gaatcctcgc
    32641 agccccggaa ccgacaggca cccctcccag tagcccgcgg tggcgggatc cacgacctcc
    32701 acggacgggt tgatgaacac cgtcagcggc agagcgggga tctcgccgta gcgggtgggg
    32761 ccgccgggaa tctcgatgat ggccagccgc acggactcgt tgatctgcgg ggccgcgaga
    32821 cctatgccgc cgtagtcgtg aagggtgtcg atcatgtcgc cgatcagacg gcccaccgcc
    32881 tcgctgcgga tctcttcgcc ggccagcggc ctggcgaccc ttcggagggt ggggtgtccc
    32941 atgcggatga ttttgcgaac ggccatgggc ggagacgata cggggtcagg tgaacgcttg
    33001 ccagccgcgc tcggtcacga tgccgtccag agggatgtcc cagtcctgga acggcagcgg
    33061 gtcggcgctt cgctgcactt cgtgggcgat gccgacgatc cggggccgaa gcggttcggg
    33121 cagcgcgccc aggaaccggt cgtagtagcc cgcacccatg cccaggcgaa caccgtactc
    33181 gtcgaaggcg accagcggga gaaggatcag gtccaggctt ctgccgtcca cgaaagccga
    33241 gccggggccc ggctcgggaa tcccgtagcg gttcaccagc atgggcgcgg ccctatggta
    33301 acggtaaaag gccattctcc ctgcggtgct gactaccggc agagcgagct ggcggtttcg
    33361 gtactccagc tcgccgagca ggggcgcgag gctcacttct ccatcgttgg cccagtacgc
    33421 gccgattcgc ctgctgcgcc atgacacggg gttgttgagc aggtgtcggg ctacccggag
    33481 cgcgtgctct cgctgctgat gagagccgag ggcccgcctg cgctcacgga agaaacggcg
    33541 aagttcctga cgttcgaggg tgctggtcat ggcagggaac cgggttggaa agagtctccc
    33601 gaaaatgccg ctgtttggtt tgccctgaac cgttgtggtt caaggcgggg acccgggcga
    33661 cgtctaaggc ttcccgatct gcaccaccgt ttggataacc gcgggcggta tcggacaggc
    33721 tcaccggcgt cagcgccgag gtctcctata gtttatctaa cggctcaagg acgacaccaa
    33781 ctggcgaaca ttccaggaga cagcacggag tatagcccaa ttgacgtcgt tgccacgggt
    33841 tgacaaccgg tcacgatcga gaactgtgaa accgttaaag gttcagctgt ttctgttccg
    33901 agagcgcgcg gccgacccgc tcggtgagct cggtcacctt ctggctgacg acgccgtgag
    33961 tggattccaa agacttggag ctgttgatga actcgttggc gatgttcagc gcggccatca
    34021 cggcgatccg gtccatcccg aaaatcttgc cggactcgcg tatggaacgc atctgctcgt
    34081 caaggtagcg ggcagacctc gagagcgcgt ccacctcttc ggcgggacag ctgacctgat
    34141 actccttgtc gaggatcttc acgcttacgg tggtggcttc ttcgctcatg tcccctgctt
    34201 cgctaatcct gctcgagcgc cttcagtcgg gtgatcatcg attcgacccg ggctttggcc
    34261 agctcgtttt tctcaatgag cttcgcccgc tccgtggtcc agttctgctg ttgtgctcgc
    34321 agcgcccgat tctccttggt cagcacgctg catagctcta tgagctcatc cactttgctc
    34381 tcgagcgccg tccattctgt gtcggccatg ccaggcttca cgcgtattct gcttatcgca
    34441 ggatagtgtc gcctgaatgg gctggcatgt gaagcatgta acttctgcta gcattaggcc
    34501 tatcccgggg cacgaagctc gataggcgac atggcggacc tgacggcgct cgcacttggt
    34561 gcggtggaat ctctttcggt tgccgagatc cacggcgccg tgtgcgggct ggcagtcgcc
    34621 tccgaggggg gaatcgagct gggcgctctc gtcgagctgg tgggcatcga ggcccttcgg
    34681 gacgaacact ccgttggaga atttgttaac gccgccgtgg acgcgctcca cgccgaggat
    34741 ctcagctttg ctccgctcct cccggacgac catgtggacc tggcgctgcg cctggaagcg
    34801 ctgggcgaat ggtgcggcgc gttcctggcc ggctttgctt caggatcccg atttcaggaa
    34861 ctcggagaag agcagattcc ggaggaggcc cgggaaatca tcggcgactt cacctatatc
    34921 gccaacgttg acggctcgga ggccggcgac ggggaggatc cggaatccgc ggagagggac
    34981 tatgccgagg tgcacgagta cgtgaaggtc ggcgcgttgc ttctgcgcac cctcctgggt
    35041 ggcgacgcgc ctgaagagac ggcgcggccg tgattgccgc tgcggagtac cggcgccgcc
    35101 gtcagcgcct catgagcctg atggacgaag ggtccatcgc cgttctgccg gcggccggcc
    35161 agcgtttccg taaccgcaac acggagtatc cgttccgcca ggacagtgac ttctactatc
    35221 tgacgggctt tgcggagccc gacgccgttc tggtgctggc gcccgggcgt gcgcacggtc
    35281 aggtcatcct cttctgcaga gagcgcgagg cgcgcgccga gcgctacga
//
LOCUS       DBNHLM010000375         4551 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_178576, whole genome shotgun sequence.
ACCESSION   DBNHLM010000375 DBNHLM010000000
VERSION     DBNHLM010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_178576"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /gene="crtI"
                     /locus_tag="AC16QF_17510"
     CDS             <1..1004
                     /gene="crtI"
                     /locus_tag="AC16QF_17510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007253234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR6450409.1"
                     /translation="EQLRQAFSFHTLLVGGNPFNTSSIYTLIHSLEQRWGVHFPKGGT
                     GALVQGLVRLFGDLGGELKLNTPVAEILTSGDRVTGVRTDNGWEGRFDAVASNADVVF
                     TYERLLGRTARGRAAARALRRKRFSPSLFVVYFGVRGSYPELKHHSILFGPRYRELLK
                     EIYAGPGLPDDFSLYLHAPSVTDPSLAPPGCSTFYVLSPVPNLKAADLDWSELGPRYR
                     DHILAYLEKHYLPGLGERLVTTRTFTPDDFQTQLNAHLGSAFSLEPVLWQSAWFRTHN
                     RDDVLKGLYFAGAGTHPGAGIPGVVGSAKATSRLMLDDFGRTYPDSDRSDQFAKEMA"
     gene            1005..1934
                     /locus_tag="AC16QF_17515"
     CDS             1005..1934
                     /locus_tag="AC16QF_17515"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014597913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HIR6450410.1"
                     /translation="MSDVFAASASIISRGSKSFAAAARLFDPTTRGHAIMLYAWCRHC
                     DDEIDGQDLGIGQRSPAPPEQRARLEQLRRDTEAAMRGEAVDGTPFVAFQRVFLECDI
                     PRNYPLELLDGFAMDVEGHECRTLSDTLRYCYHVAGTVGGMMATVMGIRDPEVVARAV
                     DLGIAFQLTNIARDVMQDAADGRCYLPRQWLADAGVPEDTGQFPDHRDVIYGQTIRIL
                     DEAERYYESARHGIGRLRFRNAWAIASALAIYRDIGRVVRRDGPDVWQHRATVGVGRK
                     LVLLLKGAALAGLQVTFGRRRTAPIRRGLWSAS"
     gene            complement(1923..2408)
                     /locus_tag="AC16QF_17520"
     CDS             complement(1923..2408)
                     /locus_tag="AC16QF_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020305761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR6450411.1"
                     /translation="MTVVVNAACFLTALAIMEWVAWVTHKYVMHGFLWSLHESHHQPR
                     KGRFERNDWFAVFFAVPSIVLIYLGNNGYPAALWGGLGVAAYGLIYFIFHDVIVHRRL
                     DTGYAPRSRYMRRIVHAHRLHHAVSEKHGAVSFGFVYAPPIPVLRAEMKALAASGASG
                     S"
     gene            complement(2405..3307)
                     /locus_tag="AC16QF_17525"
     CDS             complement(2405..3307)
                     /locus_tag="AC16QF_17525"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR6450412.1"
                     /translation="MDAIRHSVEARLRELSPSAAEGGERIEHAIQYSLLDGGKRLRPL
                     LTILTASTFDADPDLALDPACAIEMVHTASLVLDDLPCMDDALQRRGKPATHVAHGED
                     IAILSAVSLISEAFGVIARAPGIAPEVRTSIVGRLADAIGIQGLSAGQERDLHEAQST
                     TSRAALEKRHHQKTGALFVACLEAGARIAGVDETTMAAMTKFGERVGIGFQILDDLLD
                     LSASESTGKDAHRDEGKATFATVMTIQEAEREAITCLDNALAELADQIADAGPFAVFV
                     DLVVRAYADQVSRRGSEIAGALRP"
     gene            complement(3415..>4551)
                     /locus_tag="AC16QF_17530"
     CDS             complement(3415..>4551)
                     /locus_tag="AC16QF_17530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR6450413.1"
                     /translation="WWLLALSVLLRYLHDHQPRYMILLGVIFGLALLTKLAIAAFILC
                     LLGTSALYARWMFYRTESWAGSAIALSMLAPFVMWQAVSGWPLLEFVQAYNVSEPEPM
                     VLDQPLFGLIATMNPPFLLIWLPGLIYSLMAPKPIRVVGSAAVATLVFFLAAGVKFYF
                     ATPLFALFTALGAVLWQRWLSSSGARSCLLAILLISGVAAVPIAAPVLAPTMLQQVAN
                     FIRDAEQDKVADDDAPIGRYFPHFAEMHGWPELTEWVTRAYEGLPSARRPEVAVVAAY
                     FGQAGALNQLDKADRLPVVHSGHMNYYLWWQADEPNARLEDVLLVGFHPKELEPLFAE
                     ITILDRFSCNRCMTREQGAYLIRARRPLIDPEEIRDRLKRFHFF"
BASE COUNT          843 a         1414 c         1493 g          801 t
ORIGIN      
        1 acgagcagct gcggcaggcg ttcagctttc acacgctgct ggtcggcggc aatccgttca
       61 acacctcgtc catctacacg ctgattcact ccctcgagca gcgctggggc gtgcactttc
      121 caaagggtgg aaccggcgct ctggtgcagg ggctggtccg ccttttcggc gatcttggag
      181 gcgagctcaa gctcaacacg ccggtggcgg agatattgac cagcggcgac cgcgtcacgg
      241 gtgtccggac ggacaacggc tgggagggac ggttcgacgc cgtggccagc aacgccgacg
      301 tggtgttcac ctatgaaagg ctgcttggcc ggacggcgcg gggcagagcg gccgcccgcg
      361 cgctgcggcg caaaagattc agcccctcgc tgttcgtggt ttactttggc gtcaggggca
      421 gctaccccga actgaagcac catagcattc tcttcggtcc gcgttaccgg gagcttctca
      481 aggaaattta cgccggtccc gggctgccgg acgatttctc gttgtatctg cacgcgccca
      541 gcgttaccga cccctctctc gccccgccag gctgctcgac cttctacgtg ttgtcgccgg
      601 tacccaatct caaggcggcc gacctcgact ggtccgagct tggcccgcgt taccgggatc
      661 acattcttgc gtacctggaa aagcactatc tcccggggct cggtgagcgt ctggtcacca
      721 cccgcacgtt cacgccggat gactttcaga cgcagctcaa cgcgcatctt ggctcggcgt
      781 tttctttaga acccgtgcta tggcaaagcg cctggttccg cacgcacaac cgcgacgacg
      841 tgctcaaagg cctttacttt gccggagcgg gcacgcaccc gggcgcgggg atacccggcg
      901 tggtcggctc cgccaaggcc acatcgcgcc tgatgctcga tgacttcggc cgaacgtatc
      961 cggacagcga ccggtcggat cagttcgcca aggagatggc ctgagtgagt gacgttttcg
     1021 cagccagcgc gtcgatcatc tccaggggct cgaaaagctt cgctgcggcc gcgcgcctgt
     1081 ttgacccgac gacccggggt cacgcgatca tgctatatgc gtggtgccgc cactgcgatg
     1141 acgagatcga cggccaggat ctcggcatcg ggcagcgctc tccggcacct ccggagcagc
     1201 gcgcgcggct ggagcagttg cggcgggata ccgaagcggc catgcgcggt gaggcggtcg
     1261 acggaacccc gttcgtcgcc tttcagagag tctttctcga atgcgacatt ccccggaact
     1321 acccgctgga attgctcgat gggttcgcca tggacgtcga gggtcatgag tgccgcacgc
     1381 tcagcgacac gctgcgttac tgctatcacg tggcagggac cgtgggcggg atgatggcga
     1441 cggtcatggg gattcgtgat ccagaggtgg tggctcgggc cgtcgacctg gggatcgcgt
     1501 ttcaactgac caacattgcc cgcgacgtca tgcaggacgc tgccgacgga cgctgctacc
     1561 tgcccaggca atggctcgcc gacgccgggg tgcccgagga caccgggcag tttcccgacc
     1621 accgcgacgt catctacggt cagaccattc ggattctcga cgaggccgag cgctactacg
     1681 agtcggcaag acacggcatt ggccgcctta ggtttcgcaa tgcctgggcg atcgccagcg
     1741 cgcttgccat ctatcgcgac attggtcggg tcgttcgccg tgacgggcct gacgtctggc
     1801 agcaccgggc gaccgtcggc gtaggccgaa agctcgtgct gcttcttaaa ggagcagcgt
     1861 tagcgggact gcaggttacg ttcgggcgac gccggacggc ccctatcaga cgtgggctct
     1921 ggtcagcttc ctgacgcccc ggaggccgcc agggctttca tctccgcccg caatacggga
     1981 atcggcggcg cgtagacgaa gccgaaagat accgcgccat gcttttcgga cacggcgtgg
     2041 tgaaggcgat gcgcgtgaac gatacgacgc atgtagcggc tgcgcggcgc gtaaccggtg
     2101 tcgaggcgtc ggtggacgat cacgtcgtga aagatgaagt agatcagccc gtaggccgcg
     2161 acgccgagcc cgccccagag cgcggccgga tagccattgt tgccgagata gatgagcacg
     2221 atggacggta cggcaaagaa cacggcaaac cagtcgttgc gctcgaagcg cccctttcgg
     2281 ggctgatgat gcgattcgtg caggctccag agaaacccgt gcatcacgta cttgtgggtg
     2341 acccaggcca cccattccat gatggcgaga gcggtcagga aacaggcggc gttgacgacg
     2401 acggtcacgg ccgcaacgcg ccagcgatct cggaccccct cctgctcacc tgatcggcgt
     2461 aggcgcgaac caccagatcc acgaaaacgg cgaacggccc cgcgtctgcg atctggtcgg
     2521 ccagttcggc aagcgcgtta tcgaggcagg tgatggcttc acgttcggct tcctgaatcg
     2581 tcatcacggt ggcgaaggtg gctttgccct cgtcgcgatg agcgtcctta ccggtggact
     2641 cggatgcgga caggtccaga agatcgtcca gaatctggaa accgatgccg acccgctcgc
     2701 cgaatttcgt catcgcggcc atggtggtct cgtccacgcc ggcgatgcgg gcgccggctt
     2761 ccaggcacgc cacgaacaga gcgcccgttt tctgatgatg gcgcttctcg agagcggccc
     2821 ggctggtcgt actctgggcc tcatgcagat cgcgctcctg cccggcagag agcccttgga
     2881 ttccgatcgc gtccgccagg cggccgacga tgctcgttcg cacttccggc gcgatccccg
     2941 gcgcccgggc gattacgccg aacgcctcgc ttatcaggga aaccgccgag aggatggcga
     3001 tgtcctcgcc gtgggcgacg tgcgtcgccg gcttgcctcg acgctgaagg gcgtcgtcca
     3061 tgcagggcag gtcatccagg acgagcgagg cggtatgtac catctcgatt gcgcaggctg
     3121 gatccagcgc cagatcgggg tctgcgtcga atgtcgaagc agtcaggatg gtgagcagcg
     3181 gcctgagccg cttgccgccg tccagcaggc tgtattggat ggcgtgctcg atgcgctcgc
     3241 cgccttcggc agcggacggc gaaagctcgc gaaggcgcgc ttccaccgag tggcggatcg
     3301 cgtccaactt cgtaatgcta tctggcgccg cctgctccag gccaagtctg gcttcactca
     3361 atttggcaaa accttgtctg ggacaggtca ttttgcctac atctgcccgg cgactcaaaa
     3421 gaaatggaag cgcttgagcc tgtcgcggat ctcttcggga tcaatcagcg gccgtcttgc
     3481 gcgaatcaga taggcgccct gttcccgcgt catgcagcga ttgcaggaga agcggtcgag
     3541 gatggtgatt tccgcaaaga gcggttcgag ctcctttggg tggaagccga cgaggagcac
     3601 gtcctcgagc cgggcattcg gctcgtccgc ctgccaccag aggtagtagt tcatgtggcc
     3661 ggaatgcacg acgggcagac ggtcggcctt gtccagctgg ttgagggcgc cggcctggcc
     3721 gaaatacgcg gcgaccacgg cgacttcggg tctccgggcg gagggcaggc cttcgtaggc
     3781 ccgggtcacc cattcggtca gctcaggcca tccatgcatc tccgcaaagt gcggaaagta
     3841 tcgcccgatg ggtgcgtcat cgtctgcgac tttgtcctgt tcggcgtccc gaatgaagtt
     3901 tgccacctgc tgaagcatgg tgggcgcgag cacgggggcg gcaatcggaa cggccgcgac
     3961 tccggagatc aggagaatgg cgagcaggca gctgcgggcg cccgagctgg aaagccagcg
     4021 ctgccagagc acggcgccca aggcggtgaa cagcgcgaac agcggcgttg caaagtagaa
     4081 cttcaccccg gcggcgagaa agaaaaccag ggtcgccacc gctgcgctcc cgaccacccg
     4141 tatgggcttc ggagccatca gcgagtaaat cagacccgga agccagatca gcaagaacgg
     4201 ggggttcatg gtggcgatga ggccgaagag aggctgatcg agaaccatgg gctccggttc
     4261 gctgacattg taagcctgga cgaactccag caacggccac cccgaaaccg cctgccacat
     4321 cacgaacggg gccaacatgc tcagagctat ggccgacccc gcccaggact ccgttcggta
     4381 gaacatccat cgtgcgtaca gggcgctggt gcccagcagg cacaagatga acgcggcgat
     4441 ggcaagcttg gtgagaagtg cgaggccgaa gatgacgcct agcaggatca tgtagcgggg
     4501 ctgatggtca tgcaggtagc gaagcagcac agacaatgca agcagccacc a
//
LOCUS       DBNHLM010000376         7401 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_297047, whole genome shotgun sequence.
ACCESSION   DBNHLM010000376 DBNHLM010000000
VERSION     DBNHLM010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7401)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_297047"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="AC16QF_17535"
     CDS             <1..371
                     /locus_tag="AC16QF_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HIR6450414.1"
                     /translation="DVGPVVAGNIHSFFANDDGARAVDALLEAGIRWPAVAVETGAQP
                     LLGQTWVLTGTLESMTRDAAKSELVALGAKVSGSVSGKTSQVVAGPGAGSKLEKARDL
                     GVPVMDEAEFLEVLESHRHG"
     gene            364..981
                     /locus_tag="AC16QF_17540"
     CDS             364..981
                     /locus_tag="AC16QF_17540"
                     /inference="COORDINATES: protein motif:HMM:NF039960.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR6450415.1"
                     /translation="MGRARVARAAVLGLSVVVMAGCASAPPSKPDQICSIFREKSGWY
                     KDARRAEKRWDMPISVGMAFVYKESSYRSDARPERERLLWVVPWRRPSSAFGYAQITD
                     AAWREYLDDTRGFLRERDDFGTALDFVGWYNDRSHRRLGIAKNDAYNLYLAYYVGHGG
                     YKRGHHRKKPEVRRYARRVSEKSKRYAAQLSRCEKEFRKPWWRPI"
     gene            1027..3120
                     /gene="dinG"
                     /locus_tag="AC16QF_17545"
     CDS             1027..3120
                     /gene="dinG"
                     /locus_tag="AC16QF_17545"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008729.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HIR6450416.1"
                     /translation="MSEALLNDELKGVIQTGYRAWLDARGFRPRRGQREMIALIARTL
                     TGDAPRIVAVEAGTGTGKTAAYCLAAIPTGAALDKTVVISTATVALQEQVVLRDLPDL
                     ASRSGLTFSFTLAKGRGRYVCLKRLDDRLRFAEPSEIPLFEDTGEEGAALYQAMLNAF
                     ANKTWNGDRDEWTEEVDPQLWRAVTTDHRGCSNNRCGFFRQCPFFKARGALDAADVIV
                     ANHDLVLADLSLGGGVVLPPPEDCIYVLDEAHHLPEKTQQHFSASTRVLATGQWVDNV
                     ARVIGSLTQRFGRPDELVSLASRASEHGAAFAEAMMRMGDDLQGLSFEPRGEGLATHR
                     FRLGQVPAELAEQAQLALEPLTLLAGVISAVHELLQKAVAGELPWDNGFEAEDWLPAV
                     GQLETRALATMALLEDFGRGAAEFSKDGSPQGLKARWVNETAGDLEMVSAPLQPGQLL
                     QEALWSSCYAAVCTSATLTALGTFDRFLEQVGLGSVNAVRIPSPFDFQRLATLTVPSM
                     RSDPREFEAHSQEVAALLPGLLADDPGALVLFTSWRQMHEVIGQLPEDLLDKLQVQGE
                     ASKQALLSRHRSAVDEGRQSVLVGLASFAEGVDLPDDYCRHVVIVKLPFAVPDDPMDQ
                     AMAEWVEARGRNAFFEIAVPDAALKLVQACGRLVRHEGDFGRITLLDRRIVTARYGRA
                     LLDSLPPYRRELNPS"
     gene            complement(3107..5911)
                     /locus_tag="AC16QF_17550"
     CDS             complement(3107..5911)
                     /locus_tag="AC16QF_17550"
                     /inference="COORDINATES: protein motif:HMM:NF027513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HIR6450417.1"
                     /translation="MLKLIPLALLSLVLGSCANEPARPPAPDPGAPVVIAKSPNDERS
                     YRYLVLDNELRVLLVSDPAADMAAASLVVLRGSFHEPAEFAGLAHFLEHMLFIGTEKY
                     PEVDSFKQFVAAQGGSSNAYTANDHTNYFMEVRTEAFREALDRFAQFFISPLIDPAYT
                     EREKNAVHSEYQLQLKDDGWRGLHAFKAAMSPEHPGSRFSLGTLSTLGPGVDDALKKF
                     REENYSADQMILTALSNEPLDELESWVAPMFDAIENRSIGPAKPLPEAFPRERLPLTL
                     SYRPTRDRRSVTYQFPVPAPDDYYRQKPAMYVTNLLGHEGAGSLHARLKAAGWIESLS
                     SAVYRFDEQNSFITVDMDLTQTGSGHVDEITDLLFAYIDMLKGHSPEAWRYEEQAKVA
                     RLGFRFQEKSGPTAFVYRVTPLLDRFPPQDVLAAPYLMTEFDPKLIRRYISYLTPENL
                     LMQMVGPDAPTDRVEPWFEVPYGLTRTQRRPDPTEDPALGLPGPNRFLPDSLELLAAD
                     GDGPRLAIDDAGLSLWLDRDTEFGTPRANVYLLLGLRDGLESPRDIAMAQLYQRLVND
                     ASNQLAYPAWLAGLSYRLSLAPQGFQLIISGYSDKQAALLDAVIENLTDVPIDAAKFE
                     LYQAELVREWRNFAQERPVTQTYSALWFLLLSSSWPPEALASALENAKPEDLAAWRSK
                     RLDVFDVTGLAHGNVDAAAIDDIADLIREHLPQQGFGLSQPQVRTVAESLKLAVPVDH
                     PDAAMILHVQDPDTSYEARARSVMAAHLLSEPYFTSLRTEQQLGYVVDITARALRRRG
                     AITFLIQSPVASPAQLEAATRQFVTDQLAALQDMPTEQFEESRAGLIARLTEQDSNLG
                     QRSQRYWADIETGVTSLDSRERIAEAVNGLAVSDMIAYLKNLQQKLDDQRLLIYHDGQ
                     FPPGPLGGREIESVQAFKRG"
     gene            complement(5949..6275)
                     /locus_tag="AC16QF_17555"
     CDS             complement(5949..6275)
                     /locus_tag="AC16QF_17555"
                     /inference="COORDINATES: protein motif:HMM:NF019301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HIR6450418.1"
                     /translation="MTVSPPLPLGSTASWFLASWTEFLGGILLLFGLGTRLVAKAKDI
                     LREHGDYRYLNGSGSIVKLNGGIEFAAIYIAMLLALLVIGGGRYLSVDYDVGLLGRTR
                     ASAQRE"
     gene            6408..7289
                     /locus_tag="AC16QF_17560"
     CDS             6408..7289
                     /locus_tag="AC16QF_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450419.1"
                     /translation="MAGLTLDDLAQALLAGSEPWTDPGALAARLDPDIPEDWRNLAAV
                     VVAAGATGTIGVGGGQGAGKSTLAAAMVEASRLAGRKAQACSLDDFYLTRQERLALAR
                     TVHPLFVTRGVPGTHDVDLALSVLRGLEGVGVTRVPVFDKGRDDRAPGPEWRAVEGPV
                     ESIVFEGWCLGAPPEPAERLIEPMNSLEREEDANRTWRSHVNDVLGESYQRLWSAMDR
                     LLYLQVPDMAAVHDWRAQQELALPPSRRMSGGQLDRFLAHYERLTRWMLETLPERADV
                     VVELGRDHRISGVRRNS"
BASE COUNT         1216 a         2295 c         2593 g         1297 t
ORIGIN      
        1 cggacgtggg cccggtggtc gccggcaaca ttcatagctt ctttgccaac gacgacggcg
       61 cccgggccgt agacgcgctg ctggaggcgg gaatccggtg gccggcggta gccgtcgaga
      121 ccggcgcgca gccgctcctc gggcagacct gggtgctgac cggcacgctg gagagcatga
      181 ccagggatgc ggccaagtcg gagctcgttg ccctgggcgc caaggtgagc ggctcggtgt
      241 cggggaagac gtcccaggtg gtggccggcc cgggcgccgg cagcaagctg gaaaaggcgc
      301 gcgacctggg ggtgccggtg atggacgaag ccgagtttct cgaggtcctg gagagccacc
      361 gccatgggta gggctcgcgt cgctcgggcc gccgttctcg gcttgagcgt ggtcgtgatg
      421 gccgggtgcg ccagcgcgcc gccgtcgaag ccggatcaga tctgctcgat cttccgggaa
      481 aaatccggtt ggtacaaaga cgccaggcgt gccgagaagc gctgggacat gcccatctcc
      541 gtgggcatgg cgttcgtcta caaggagtcg tcctaccggt ccgacgcgag gccggagcgt
      601 gagcgcctgt tgtgggtagt gccctggcgg cggccgtcct cggcgtttgg ttacgcacag
      661 atcaccgatg ccgcctggcg ggaatacctc gacgacacca gagggttcct gcgcgagcgc
      721 gacgacttcg gcacggccct ggatttcgtc ggctggtaca acgaccgcag tcaccggcgt
      781 ctcggcatcg ccaaaaacga tgcctacaat ctctatctcg cctactacgt cggccatggc
      841 ggctacaagc ggggacatca ccggaagaag ccggaggtcc gccgttatgc tcgacgggtc
      901 tcggagaaga gcaagcgata cgcggcgcag ctgtcgcgct gcgagaaaga gttccgcaag
      961 ccctggtggc ggccgatttg agcgttgccg gtggccagtg atcgaccggc aacgtaagct
     1021 cgctcgatga gcgaggccct cctcaacgac gagctgaaag gcgttattca gaccggatac
     1081 cgtgcctggc tcgacgcgag gggctttcgg ccccgacgcg gccagcggga gatgatcgcg
     1141 ctgatcgccc ggacgctgac cggtgacgcg ccgcgcatcg tggccgtgga ggcgggcact
     1201 gggacgggca agacggcggc ctattgcctg gcggccatcc ccacgggcgc tgctctggac
     1261 aagacggtgg ttatcagcac cgcgacggtg gccctgcagg agcaggtggt cctgcgcgac
     1321 ctccccgatc tggcgagccg ttctggcctg acgttcagtt tcaccctggc caaagggcgg
     1381 gggcgctacg tctgcctgaa gcggctcgac gatcggctgc gatttgcgga gccctctgag
     1441 atccccctgt tcgaggacac gggcgaggag ggcgcggccc tttaccaggc catgctcaac
     1501 gcgtttgcga acaaaacctg gaacggcgat cgcgacgagt ggaccgagga ggtcgacccg
     1561 cagctgtggc gggccgtgac cacggaccat cggggatgca gcaacaatcg gtgtggcttc
     1621 tttcggcagt gcccgttctt caaggccagg ggagcgctcg acgccgccga cgtcatcgtc
     1681 gccaaccacg acctggtgct ggccgacctc agcctcggtg gcggcgtcgt tctgccgccg
     1741 ccggaggact gcatctatgt tctcgacgag gcgcaccatc tgccggagaa aacccagcag
     1801 catttctcgg cctccacgcg ggtcctcgcc accgggcagt gggtggacaa cgttgcccgg
     1861 gtcatcggca gcttgacgca gcgtttcggg cgaccggacg agctcgtcag tctcgccagc
     1921 agggcctcgg agcacggcgc cgcgttcgct gaagccatga tgcgcatggg ggacgatctc
     1981 caggggctca gcttcgaacc gcgcggtgaa ggcctggcaa cgcaccgctt caggctggga
     2041 caggtaccag cggagctggc agagcaggcg cagctggcgc tggagccgct cacgctcctt
     2101 gccggcgtga tctccgccgt ccacgagctg ctgcagaaag cggttgccgg cgagttgccc
     2161 tgggacaacg gcttcgaggc ggaggactgg ctgccggccg tggggcagct ggaaacgcgc
     2221 gcgcttgcga ccatggctct gctggaggat ttcggccgcg gcgcggcaga gttttcaaaa
     2281 gacggctcgc cccagggatt gaaggcccgc tgggtcaacg agaccgcggg tgatctggaa
     2341 atggtcagcg ccccgctgca gcccggccag cttctgcagg aggcgctctg gtcgagttgc
     2401 tacgcggccg tgtgcacctc ggcgacgctg actgctctgg gcaccttcga ccgctttctg
     2461 gagcaggtgg gcctgggttc ggtcaacgcg gtccggattc cgagcccatt cgactttcag
     2521 cggctggcga cgttgacggt gccgagcatg cgctcggacc ccagggagtt cgaggcgcac
     2581 agccaggagg tggccgcgct cctgccggga ttgcttgccg atgacccggg cgcgctggtg
     2641 ctgttcacgt cctggcggca gatgcacgag gtgatcggac agctcccgga ggacctgctg
     2701 gacaagctgc aggttcaggg cgaggcctcc aagcaggcgc ttctatcccg gcaccgcagc
     2761 gcggtggacg agggtcggca gagcgtcctg gtcggccttg ccagcttcgc cgagggtgtc
     2821 gacctgcccg acgactactg ccggcacgtg gtcatcgtca aactgccctt tgccgtgccg
     2881 gacgatccga tggatcaggc catggccgaa tgggtcgaag cccgcgggcg caacgcgttc
     2941 ttcgagatcg cggtgccgga cgcggcgctc aagctggtcc aggcatgcgg gcgcctggtc
     3001 agacacgaag gagacttcgg ccggatcacg ttgctcgaca gacgcatcgt cacggcgcgt
     3061 tacgggcgtg ccctgctcga ctcgctgcct ccctacaggc gggagctcaa cccctcttga
     3121 aggcctggac gctctcgatc tccctgccgc caagggggcc cggcggaaat tggccgtcgt
     3181 gataaatgag cagtcgctgg tcgtccagct tctgctgaag gttcttcaga taggcgatca
     3241 tgtcgctcac ggcgaggccg ttcacggcct ctgcgatcct ttcccgggag tcgaggctgg
     3301 ttacgcccgt ttcgatatcc gcccagtagc gctggctgcg ctggccgagg ttgctgtcct
     3361 gctcggtcaa ccgcgcgatg aggcccgccc ggctctcctc gaactgctcg gtcggcatgt
     3421 cctgcagggc ggccagctga tcggtgacga actggcgggt ggccgcctcg agctgagccg
     3481 ggcttgccac cggtgactgg atcaggaagg tgatggcgcc ccgtctgcgg agcgcccgag
     3541 cggtgatgtc caccacgtag ccgagctgct gctccgtgcg caggcttgtg aagtacggtt
     3601 cgctcaggag gtgcgcggcc atcacgctgc gggcgcgcgc ctcgtagctg gtgtccggat
     3661 cctgcacgtg aagaatcatc gccgcgtccg ggtggtccac ggggaccgcg agcttcagtg
     3721 actcggcaac ggtgcggacc tggggctggc tgaggccgaa accctgctgg ggcaggtgct
     3781 cgcggatcag atcggcgatg tcgtcgatgg cggcagcgtc cacgtttccg tgcgccaggc
     3841 cggtcacgtc gaaaacgtcg agcctcttgc tgcgccaggc cgccaggtcc tccggctttg
     3901 cgttctccag ggcagatgcg agcgcctcgg gaggccagct gctcgacagc agcagaaacc
     3961 agagcgcgct gtaggtctgg gtgaccggac gttcctgggc gaagttgcgc cactcccgca
     4021 ccagttccgc ctggtagagc tcgaactttg cggcatcgat gggtacgtcg gtgaggtttt
     4081 cgatcaccgc gtcgagcagg gcggcctgct tgtcgctgta acccgagatg atgagctgaa
     4141 aaccctgggg tgcgagagac agtcggtagc tcaggcccgc gagccaggcc gggtaggcca
     4201 gctggttgct ggcgtcgttg acgaggcgct gatagagctg ggccatggcg atgtcgcggg
     4261 gggactcgag cccgtcacgc aggcccagca gcaggtagac gttggcccgt ggcgtgccaa
     4321 actcggtatc gcggtcgagc cacagcgaca ggccggcgtc gtcgatggcc agccgcggcc
     4381 cgtcgccgtc cgcggcgagc agctcgagag aatccggaag gaaccggttc ggccccggca
     4441 ggccgagggc ggggtcctcc gtcggatctg gtcgccgttg ggttcgggtc aggccgtagg
     4501 gcacctcgaa ccagggctcc acccgatcgg tgggcgcgtc gggccccacc atctgcatca
     4561 gcaggttctc gggcgtgagg taactgatgt aacgacgaat cagcttgggg tcgaactcgg
     4621 tcatcaggta cggcgcggcc aggacgtcct gtggcggaaa acgatcgagc aggggcgtca
     4681 cccggtagac gaaagcggtg ggcccggatt tttcctggaa ccggaagccg agcctggcca
     4741 ccttggcctg ttcctcgtag cgccaggcct ctggcgagtg ccctttgagc atgtcgatgt
     4801 aagcgaacag caggtcggtg atctcgtcga cgtgcccgct gccggtctgg gtgagatcca
     4861 tgtccacggt gatgaacgag ttctgctcgt cgaaacggta gacggcgctg ctcagcgact
     4921 ctatccagcc tgcggctttc agccgggcgt gaaggcttcc cgcgccctcg tgccccagca
     4981 ggttcgttac gtacatggcc ggtttctggc ggtagtagtc gtcgggggct gggacgggaa
     5041 actgataggt gaccgagcgc cggtcgcgcg ttggccggta gctcagggtc aacggcaacc
     5101 gctcccgggg aaaggcttcg ggaaggggct ttgcagggcc gatggatcgg ttctcgatgg
     5161 cgtcgaacat gggcgccacc cagctctcca gctcgtcgag aggttcgttg gagagggcgg
     5221 tgaggatcat ctggtctgcc gagtaattct cctcgcgaaa cttcttcagc gcgtcatcca
     5281 cccccgggcc cagggtcgac agggtgccga gagagaaccg ggatcccggg tgctccgggc
     5341 tcatggccgc cttgaaagcg tgaaggcccc gccagccgtc atcctttagc tgcagctggt
     5401 actccgagtg aacggcattc ttctcccgct cggtgtacgc aggatcgatg agcgggctga
     5461 tgaagaactg cgcgaatcga tcgagggctt cccggaaggc ctcggtcctg acctccatga
     5521 agtagtttgt atggtcgttg gccgtgtacg cgttggacga ccctccctgg gcggcgacga
     5581 actgcttgaa cgagtccacc tccggatact tctcggtgcc gatgaacagc atgtgctcca
     5641 gaaagtgggc gaggccggcg aactcggcgg gctcgtgaaa gctccctcgc agcacgacca
     5701 gcgaggcagc ggccatgtcc gctgccgggt cggatacgag cagcacccgc agttcgttat
     5761 cgagaacgag gtagcggtag ctcctttcgt cgttcggact cttggcgatg acgaccggag
     5821 ccccgggatc gggggccggc gggcgcgccg gttcgttggc gcacgatccc agcaccagtg
     5881 aaagcagcgc gagcggtatg agtttcagca cgtttcggtt tccagttgtt gagcggtccg
     5941 ggcggcgatc attcccgttg tgcggacgcg cgagttctcc cgagcagccc gacatcgtag
     6001 tcgacgctga gataacggcc acctccgatc accagcaacg ccaggagcat ggcgatgtag
     6061 atggcggcaa attcgatgcc gccgttcagc ttgacgatgc tgccgctgcc gttgagatac
     6121 cgatagtcgc cgtgttctcg caggatgtcc tttgctttgg cgaccagccg ggtgccaagt
     6181 ccgaacagca gcaggatgcc tccaaggaac tcggtccagg aagccaggaa ccacgaagcc
     6241 gtcgagccga gcggcaatgg tggcgagacg gtcatgccag tcagctacgg ttgctatgat
     6301 tggctggatg aggcttctcc catgtttttc agaattagac tcgaaggcgg agcagggttc
     6361 cccggagggc ggtccatgat gcacaaaccg tcaggtgatg gaaaaccatg gcgggtctga
     6421 cactcgacga cctggcccag gcgctgctcg caggttccga gccatggacg gatcccgggg
     6481 cgctggcggc ccgcctcgat cccgacattc ccgaagactg gcgcaatctg gcggccgtcg
     6541 tcgtcgctgc gggagcgacg ggcaccatcg gcgtcggcgg gggccaggga gcgggcaagt
     6601 cgactctggc tgcggcgatg gtcgaagcca gccgcctggc cggacgaaag gcgcaggcct
     6661 gctcgctcga tgatttctat ctcaccaggc aagagcgtct ggctctggca cgaaccgttc
     6721 atccgctctt cgtgacccgg ggcgttcccg gtacccatga cgtcgatctg gcgctgtccg
     6781 ttctgcgcgg gctcgagggt gtcggcgtca ccagggtgcc cgtattcgac aaaggccggg
     6841 acgatcgtgc gcccggcccg gagtggcgtg cggtcgaggg cccggtagag agcatcgtct
     6901 tcgagggctg gtgcctgggc gccccgcccg agcctgccga acgtctcatc gagccgatga
     6961 actcgctcga gcgcgaagag gatgcgaacc ggacctggcg ctcccacgtg aacgatgttc
     7021 tgggcgagag ctatcaacgg ctttggagcg cgatggatcg cctgctctac ctccaggttc
     7081 cggacatggc cgccgtgcac gactggcgcg cacagcagga gctggcgctg cccccgtcca
     7141 ggcgcatgtc gggcggtcag ctcgaccggt tccttgccca ctacgagcgg ctgacgcgct
     7201 ggatgctcga aaccctgccc gaacgggcag acgtggtcgt cgagctcggt cgcgaccatc
     7261 ggatctcggg cgttcgcagg aattcctgac ccgtaggccg tattcatgat ccgttcagtc
     7321 gtcggtcctt aatctggcgg ccgtcattgc agtcgaatgg agaatgacga tgtatgcatc
     7381 ccacacagcg aaaactctgc t
//
LOCUS       DBNHLM010000377         8870 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1375277, whole genome shotgun sequence.
ACCESSION   DBNHLM010000377 DBNHLM010000000
VERSION     DBNHLM010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8870)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8870)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8870
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1375277"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="AC16QF_17565"
     CDS             complement(<1..429)
                     /locus_tag="AC16QF_17565"
                     /inference="COORDINATES: protein motif:HMM:NF013117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6450420.1"
                     /translation="MQRIIDLLNVQLFTLGEQPVTVMQLLVVPLLIVVGILLIRWGAR
                     VISRRMMAREASPDVVHLVRRVFYIVAIAILVITALDLVNVPLTAFAFVSGAIAIGVG
                     FGAQNIINNFISGWILMWERPIRIGDFLEMGEVRGTVESIN"
     gene            559..1920
                     /locus_tag="AC16QF_17570"
     CDS             559..1920
                     /locus_tag="AC16QF_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR6450421.1"
                     /translation="MLKRSGQTFPALNVYLTRLVEGRLWLQVIIGMVLGILTGVAIGP
                     AAGLLDMETGVVVGNWLAFPGRLFLVTIQMVVVPLVFASIVRGLTGGPDMAELKRVGI
                     WVSGFFLVTTALAATIGIQITLLLEPGNHLDLGTQLMDQLSAADPGYVPTAPMLQNLP
                     DIVIDMLPGNPLNALVEGQMLQIVLFAFIIGLALISLPEATSRPLADLMASVQEVCMA
                     VVRWAMTIAPFAVFGLMAQLTSRTGLQTLIGLTAYVGTVLLGLLALLAVQLAFVRLFS
                     PYGPCRFLSAARELLLLAFSTSSSAAVMPLSMKTAMEKFRVRASVAQLVIPLGATINM
                     NGTALYQAVAAVFLAQVFGVEMTGAGLALIVAMAIGASIGSPATPGVGIVILASILAG
                     IGVPPAGIALIIGVDRILDMCRTAVNVSGDIVACIFADRWVLGERARDGAESPSPPEE
                     AVT"
     gene            complement(1936..3177)
                     /locus_tag="AC16QF_17575"
     CDS             complement(1936..3177)
                     /locus_tag="AC16QF_17575"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6450422.1"
                     /translation="MRGTIARLSALLTAVAILLIGHGLQLTLLPVHAEGLGWSASAIG
                     TSGSAYFLGFVVGCVLIPPVVGRVGHIRTFMVMGALATVALLAAGLLVTLPAWLIFRF
                     VTGFALSGLYMVIESWLVDVTPSNNRGTILALYTVICLLGMSVGQAFLGIASPMSLEL
                     FMAGAALICLAIVPVGLARVAAPHPIPSARFSPAVLLKASRVAMVCAFAGGLVTGAFW
                     TLGPVLARAFGLDAAQVGGMMGAGIIGGAAVQIPVGRLSDRTDRRLVIGGLMAAGALV
                     ALCGWFLAGEDSIALSVIMFLFGAATFCIYAVCIVHASDNADIPLVEIASGILIMNSA
                     GSIAGPIVVALLMDRFGPESFFAYVLACLAPVAVWVFYRTAVVERSREHEHASILPRT
                     TQAIAELSPSEPDVGTDTANN"
     gene            complement(3186..3935)
                     /locus_tag="AC16QF_17580"
     CDS             complement(3186..3935)
                     /locus_tag="AC16QF_17580"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6450423.1"
                     /translation="MTTLVRIDSFDFPPGRKITARYQVVSQLGAGWESEVYLIRETAT
                     GIERTAKVFFPHRNRGNKVARRTAEKLHKLRDCPALIQYVAQETITVRRQAVTVLISE
                     YVEGDLLSEFIARQPGGRMHRFEALHLLRALALALEPIHERREYHGDLHAGNVIVRRV
                     GLDFDVRLIDVYHLDQRKSLSVKDDVCDAIRIFYDALGGQRFYARQSPEIKAICRGLK
                     RSLISERFRTAGQLRRYLDTMSWEFEPRRGR"
     gene            complement(3988..4398)
                     /gene="mce"
                     /locus_tag="AC16QF_17585"
     CDS             complement(3988..4398)
                     /gene="mce"
                     /locus_tag="AC16QF_17585"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HIR6450424.1"
                     /translation="MIGKLNHVAIVVPDLESAAALYRDTLGATVSDPVPLPDHGVTTV
                     FVELPNSKIELLHPLGDGSPVANYLERNPKGGMHHLCYEVQDIEAAIAHLVAEGATVL
                     GDGKPSTGAHGLPVVFLHPKDFCGTLIELEEVAA"
     gene            complement(4427..6595)
                     /gene="scpA"
                     /locus_tag="AC16QF_17590"
     CDS             complement(4427..6595)
                     /gene="scpA"
                     /locus_tag="AC16QF_17590"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HIR6450425.1"
                     /translation="MAENPTRKSDPAGQDEATWKELAAKELRGKPLDELTWVSPEGIP
                     IKPLYTEADLEEIEHLGSFPGLEPYVRGPRATMYTNRPWTIRQYAGFSTAEESNAFYR
                     QNLAAGQKGLSVAFDLATHRGFDSDHPRVVGDVGMAGVAIDSVEDMKILFDGIPLDQM
                     SVSMTMNGAVLPVMAGYIVAAEEQGVAPKDLAGTIQNDILKEFMVRNTFIYPPEPSMR
                     IVADIIGYTAEHMPRFNSISISGYHMQEAGANAVQEVAFTLADGIEYVRAALVTGQEI
                     DAFAPRLSFFFCIGMNFFMEIAKLRAARVLWHELMSQFKPQNPTSLMLRTHCQTSGVS
                     LQEHDPYNNVVRTTIEAMAAVLGGTQSLHTNAFDEAIALPTPFSARIARNTQIIIAEE
                     SQMTRTVDPLGGSYYVESLTNSIVEAAREIIDEVEAMGGMTKAVVAGMPKQRIEEASA
                     RRQARVERGEDVIVGVNKYKLEKDDSVFDVLAVDNSKVRDAQLKRLARIRASRDETAT
                     QAALDALTEAARTGEGNLLDLSVAATRLRATVGEVSTALEKVWNRHTPVARMTPGVYA
                     DAHQEDEQFVRVRDEVKVFTELEGRPPSILVVKMGQDGHDRGAKVISNAFGDLGFEVK
                     MGPLFQTPAEAASQATDEAVNVVGVSTLAGGHKTLVPEMIEALKAAGNEDVIVVVGGV
                     IPQKDYQFLYDCGVHAIYGPGTNIPEAASDVLQLIPGKNR"
     gene            complement(6692..7102)
                     /locus_tag="AC16QF_17595"
     CDS             complement(6692..7102)
                     /locus_tag="AC16QF_17595"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HIR6450426.1"
                     /translation="MITENADQDQFITDHRWGVLTTLRQDGQPVSSVVAYARDGDTLV
                     VSTPGTTFKARSAGRDGRATLCVITNQEPFNFVSVEGRVDVETDELVRNTRLVFENIE
                     GTGFVEPEDLEGWLAAEQRVILRIHPQRVFGVIR"
     gene            complement(7188..8690)
                     /locus_tag="AC16QF_17600"
     CDS             complement(7188..8690)
                     /locus_tag="AC16QF_17600"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR6450427.1"
                     /translation="MKQKWYLLALVSAALVCQIAQAGVDTVRVSEAFREASVAANSDR
                     ELAPDQPPDQRLAVGMQLTAHVARSHTRDFIRRPLTSVGNLLAATFTGATGIVKELAL
                     DFVLVPRLASKAARPLSSSRGMDIEEWENDLDRITGTERTRGQMKLLVDGEAFFDRLI
                     AEIAAARRSIEIRTYIFDNDDYAVAIADLLRRRSREVAVTVMADGLGTIGGTLATADS
                     QPADYVMPVSMGAYLKEGSGVRLQMLGNTWFMGDHTKTYIFDERVAFLGGMNIGREYR
                     YDWHDIMVELTGPVVGELSSEAAQAAREDRLGDLALLGSTKRTPRTDGAEGGQELRLL
                     RTRPHDAQIYRAQLEAIRRAEKHIFVQNAYLGDDQIIYELAKARRRGVDVRVILPARP
                     DSELVRRSNALAINTLRRFGVRVYLYPGMTHVKAAIYDGWACFGTANFDRLSLRINKE
                     VNIATSDPAFVGSLRAQVFDVDFARSREVVDDEPTGASDHLYERIVDILL"
BASE COUNT         1589 a         2931 c         2788 g         1562 t
ORIGIN      
        1 gttgatcgat tccacggtgc cgcggacttc gcccatttcc agaaagtcac cgattcttat
       61 gggcctttcc cacatcagga tccagccgct gatgaagttg ttaatgatgt tctgagcccc
      121 gaaacccacc ccgatggcga tggcgcccga gacgaaggcg aacgccgtca gcggaacgtt
      181 gacgagatcg agggcggtga tgaccagaat ggcgatcgct acgatgtaga agacgcgccg
      241 gacgaggtgg acgacgtcgg ggctggcctc ccgcgccatc atgcgccgcg agatgacgcg
      301 ggccccccag cggatcagca gaatgccgac cacgatcagc agcggaacca ccagcagctg
      361 catgacggtc accggctgct caccgagagt aaagagctgc acgttgagca ggtcgataat
      421 tcgctgcacg tccggaccct ggccgcaaac agctgatatt gtactgcaga gcatcggtaa
      481 acccgtatag acgggccgct cacccgaatg tttcttaacc gccggaatcg cgggagctgg
      541 acggaattgg cgaacttcat gctcaagcgg tcgggacaga ccttcccggc gttgaacgtt
      601 tacctgaccc gcctggtgga aggcaggctg tggctgcagg tcatcatcgg catggtgctg
      661 ggaatcctga ccggcgtggc catcggcccg gcggccgggc ttctggacat ggagaccggt
      721 gtggtggtgg gaaactggct ggccttcccg ggccggctgt tcctggtgac catacagatg
      781 gtcgtggtac ccctcgtctt cgcctccatc gtgcgcggcc tgaccggcgg gcccgacatg
      841 gccgagttga aacgggtcgg catctgggtg agcggcttct tcctcgtcac gacggccctg
      901 gccgccacca taggcattca gatcaccctg ctcctcgagc cgggcaacca ccttgatctg
      961 ggcactcagc tgatggacca gctctcggcc gccgaccccg ggtacgtgcc gacggcgccc
     1021 atgctgcaga acctgccgga catcgtcatc gacatgcttc ccggcaaccc gctcaacgcg
     1081 ctggtcgagg gccagatgct gcagatcgtg ctgttcgcct tcatcatcgg gctcgcgctt
     1141 atctcgctgc cagaggcgac gtcccgacct ctggccgatc tcatggcttc cgtccaggaa
     1201 gtgtgcatgg ccgtcgtgcg ctgggcgatg accatcgccc cgttcgccgt gttcggtctg
     1261 atggctcagc tgacgagccg aaccgggctg cagacgctga tcgggctgac cgcctacgtc
     1321 ggcaccgtgc tcctgggtct gctggcgttg ctggcggtgc agctggcttt cgtgcgcttg
     1381 ttctccccct atggcccctg ccggtttctc tccgcggcga gggaattgct gctcctcgcc
     1441 ttcagcacct cgagctcggc cgccgtgatg ccgctctcca tgaagacggc catggaaaaa
     1501 ttccgggtgc gcgcgagcgt ggcgcagctg gtgattcccc tcggcgcgac gatcaacatg
     1561 aacggcaccg cgctgtatca ggcagtggcc gccgtctttc ttgcccaggt cttcggggtg
     1621 gaaatgaccg gcgcgggtct ggccctgatc gtcgccatgg ccatcggggc atccatcgga
     1681 tcccccgcca cgccgggcgt cggcatcgtc atcctggcaa gcatcctggc cggcatcggc
     1741 gtgcctcccg cggggatcgc gctgatcatc ggcgtggaca ggattctcga catgtgccgc
     1801 acggcggtga acgtcagcgg cgacatcgtt gcctgcatat tcgccgatcg ctgggtgctg
     1861 ggagagcgag cgagggacgg cgcggagagc ccgagcccgc ccgaagaggc cgtcacctag
     1921 atccccgagc cgctctcaat tattggcagt gtcggtgccg acgtcgggct cgctgggcga
     1981 gagctccgcg atggcctgcg tcgtgcgagg gagaatgctg gcatgctcgt gctcccgcga
     2041 acgctccacc acggcggtac gatagaagac ccagacggcg accggagcca gacaggctag
     2101 cacgtaggcg aagaagctct ccggaccaaa gcgatccatc agcagcgcta cgacgatggg
     2161 gcccgcgatg gaaccagcgc tgttcatgat gagaataccg cttgctatct cgaccagcgg
     2221 aatgtccgca ttgtcactgg cgtgcacgat gcagaccgcg tatatgcaga acgtcgccgc
     2281 accaaagaga aacatgatga ccgacagcgc gatggagtct tcaccagcaa ggaaccagcc
     2341 gcacagcgcc accagagcgc cggccgccat gagcccgccg atcaccagtc gccggtcggt
     2401 acgatccgag agccggccga cgggtatctg aaccgcggca cctccaatga tgcccgcacc
     2461 catcatcccg ccgacctgcg cggcgtccag gccgaaggct cgcgccaaaa ccggtccgag
     2521 cgtccagaaa gcgcctgtca ccagaccgcc tgcaaacgcg cagaccatgg cgacccggga
     2581 tgctttcagc aggacggcgg gggagaagcg agccgaggga atggggtgcg gtgcggccac
     2641 ccgcgccaga cccacgggca cgatcgccag gcagatgagg gcagcgcctg ccatgaagag
     2701 ctccaggctc atgggtgagg ctatgccgag gaaagcctgc cccacggaca tccccagcag
     2761 gcagatgacc gtgtagagag ccagtatcgt tccccgattg ttcgatggcg tcacgtccac
     2821 cagccagctt tcgatcacca tgtagagacc ggacagcgcg aacccggtaa cgaaccggaa
     2881 gatgagccag gcaggcaggg tcacgagcaa cccggcagcc agcagcgcaa cggtggccaa
     2941 ggcccccatc accatgaaag tgcgaatgtg gccgaccctg ccgaccaccg gtggaatcag
     3001 aacgcagccc accacgaaac ccagaaaata ggccgacccg ctggtaccga tggcggaagc
     3061 cgaccaaccc agaccctctg cgtgcaccgg caggagcgta agctggagac cgtggccgat
     3121 cagcaggatg gcgacagcgg tcagcagagc gctcagacgg gcgatcgttc cgcgcatggt
     3181 tctgcctacc gccctcgccg gggctcgaat tcccaggaca tggtgtccag gtaacggcgc
     3241 agctgaccag cggtgcgaaa gcgctccgag atgagcgaac gcttgagacc gcgacagatc
     3301 gccttgatct caggcgattg ccgggcgtag aaacgctggc cgcccagggc atcgtaaaag
     3361 atccggatgg catcgcagac gtcgtccttt acgctcaacg actttcgctg atcgaggtgg
     3421 tagacgtcga tgagccggac atcgaaatcg agaccgaccc gacgcacgat gacattgccc
     3481 gcgtgcagat cgccgtggta ctcgcgacgc tcgtgaatcg gttcgagcgc cagcgccagg
     3541 gcccgcagca ggtgcagcgc ctcgaagcgg tgcattcgcc cacccggctg ccgcgcgatg
     3601 aactcgctga gcagatcgcc ctccacgtac tccgagatga gcaccgtcac ggcctgccgc
     3661 ctgacggtga tcgtttcctg ggccacgtac tggatcagcg ccgggcagtc cctgagcttg
     3721 tgtagctttt ccgccgtgcg gcgcgccacc ttattgcccc ggtttcggtg cggaaagaac
     3781 actttggccg tccgctcgat accggtggcc gtctcgcgga tgagatagac ctcactttcc
     3841 cagccggctc caagctggct gacgacctga tatcgggccg tgatcttgcg gcccggcgga
     3901 aaatcgaagc tgtcgatgcg gacaagggtg gtcaccgctg catcaagcct ctcgggcgct
     3961 caggggctgg ctacgatccg ggcacgttca ggcagcaact tcttcgagct cgatcagcgt
     4021 gccgcagaaa tccttggggt gcagaaagac cacgggcagg ccatgagccc ccgtcgacgg
     4081 cttgccgtcc ccaagcaccg tggcgccctc cgcgacgagg tgcgcgatgg cggcttcgat
     4141 gtcctggacc tcgtagcaca ggtgatgcat cccgcccttc gggttccgct cgaggtagtt
     4201 cgccaccgga gatccgtcac ccagcggatg tagcagctcg atcttgctgt tgggcagctc
     4261 gacgaagacc gtcgtgacgc cgtgatccgg gagcggcacg ggatcggaca ccgtggcgcc
     4321 cagcgtgtcc ctgtagagtg ccgccgcgga ttcgaggtcc gggacgacga tggcaacgtg
     4381 gttcagctta ccgatcattt gctcttccgt tccgtgttca ggttgttcat cggttcttgc
     4441 ccggaatcag ctgcagcacg tcgctggccg cctccggtat gttggtgccc ggcccgtaga
     4501 tggcgtgcac gccgcagtcg tagagaaact gatagtcctt ctgcggaatc accccgccca
     4561 ccacgacgat cacgtcctcg ttacccgccg ctttgagcgc ttcgatcatc tcgggcacca
     4621 gcgtcttgtg tcctccagcc agggtcgaga cgcctacgac gttcaccgcc tcgtcggtcg
     4681 cctgagacgc ggcctccgcc ggggtctgga acagcggccc catcttcact tcgaagccaa
     4741 ggtcgccgaa agcattggag atgaccttgg ccccgcggtc gtggccgtcc tggcccatct
     4801 tgaccaccag gatgctcggc ggccgccctt cgagctcggt gaacaccttg acctcgtcgc
     4861 gaacccgcac gaactgctcg tcctcctgat gggcgtcggc atacaccccc ggggtcattc
     4921 gcgctacggg cgtatggcga ttccagacct tctccagcgc ggttgagacc tcgccgacgg
     4981 ttgcccgcag gcgcgtcgct gccacgctga gatccagcag gttgccctcg cccgtcctgg
     5041 cggcctcggt gagcgcgtcc agggcggcct gagtggcggt ctcgtcccgg gaggcgcgaa
     5101 tgcgcgccag ccgcttgagc tgcgcgtcgc gcaccttgct gttgtccacc gcgagaacgt
     5161 cgaagacgga gtcatccttt tcgagcttgt acttgttcac ccccacgatt acgtcctcgc
     5221 cccgctcgac ccgggcctgg cggcgcgccg aggcctcctc gatgcgctgc ttgggcatgc
     5281 cggccacgac cgccttggtc atgccgccca tggcctcgac ctcgtcgatg atctcacggg
     5341 ccgcctccac gatggagttg gtcaggcttt ccacgtaata gctgccgccc agggggtcga
     5401 ccgtgcgggt catctgggac tcttcggcga tgatgatctg ggtgttgcgg gcgatgcgcg
     5461 cggagaacgg cgtaggcagc gcgatggcct cgtcgaacgc gttggtatgc aggctctggg
     5521 tacccccgag cacggccgcc atggcctcga tcgtcgtcct gaccacgttg ttgtagggat
     5581 cgtgctcctg cagcgacacc cccgaggtct ggcagtgggt acgcagcatc agcgaggtag
     5641 ggttctgcgg cttgaactga ctcataagct cgtgccagag aacgcgcgcc gcccgcagct
     5701 tggcgatctc catgaagaag ttcatcccga tgcagaagaa gaacgacaga cgcggcgcga
     5761 acgcgtcgat ctcctgcccc gtcaccaggg cggcacggac gtactcgatc ccgtcggcca
     5821 gcgtgaaggc cacttcctga accgcgttgg cccccgcctc ctgcatgtga tagccggaga
     5881 tggagatgga attgaagcgc ggcatgtgct cggcggtgta gccgatgata tccgccacga
     5941 tccgcatgct cggctcgggc gggtagatga acgtgttgcg gaccatgaac tccttgagga
     6001 tgtcgttctg gatggtcccc gcgagatcct tgggcgccac cccctgctcc tcagcggcga
     6061 cgatgtagcc ggccatcacc ggcagaacgg cgccgttcat ggtcatggac accgacatct
     6121 gatccagcgg aattccgtcg aagagaatct tcatgtcctc cacggagtcg atggcgaccc
     6181 ccgccatgcc gacgtcgccc acgacgcgcg ggtggtccga gtcgaacccc cggtgggtgg
     6241 ccagatcaaa ggccaccgac aggcccttct gccccgcagc caggttctgg cggtaaaagg
     6301 cgttggattc ctccgccgtg gagaagcccg cgtactgacg gatggtccac ggacgattgg
     6361 tgtacatggt cgcgcgcggc ccgcgaacgt atggctcgag accggggaat gagcccagat
     6421 gctcgatttc ctcgagatcc gcttcggtgt acagcggctt gatcgggatg ccctccgggc
     6481 tcacccaggt gagctcgtcg agcggtttgc cgcgcagttc cttcgcagcc agctctttcc
     6541 aggtcgcctc gtcctgtccc gcagggtcgc tcttgcgtgt cgggttctct gccatggttc
     6601 ctacctcgag cctcaggagt cgcgcccagc gcgcaaacgg ccggcgatta tagcaacgtc
     6661 cagccccgcg gggaccggcg gatgaccccc gtcagcgaat aaccccgaac acgcgttggg
     6721 ggtgtatccg cagaatcacc cgctgctcgg ccgcaagcca gccctccaga tcttccggct
     6781 cgacgaagcc ggtgccctcg atgttctcga acaccagccg cgtgttgcga acaagctcgt
     6841 cggtctccac gtcgacccgc ccctccaccg agacgaaatt gaacggctcc tgattggtaa
     6901 tgacgcagag cgtggcccgc ccgtcgcggc cggccgaacg cgccttgaac gtcgtgcccg
     6961 gcgtgctgac cacgagcgtg tcgccgtcgc gcgcgtaagc caccaccgag ctgaccggct
     7021 ggccatcctg gcgcagcgtc gtcagaaccc cccagcgatg atcggtgatg aactgatcct
     7081 gatcggcgtt ctcggtaatc aaggcgaccc tccacaagca ttcctgtcca gaccatcgac
     7141 gatagcccaa caccgttcag cgaccaaggg tcttgcgccc gcagcgtcta caggaggatg
     7201 tccacgatgc gctcgtagag atggtcggac gctccggtgg gctcgtcgtc cacaacctcg
     7261 cgcgaccggg cgaagtccac gtcgaacacc tgggcgcgaa gcgacccgac gaacgcggga
     7321 tcggacgttg cgatgttcac ctctttgttg atgcgcaggc tgagccggtc gaagttggcg
     7381 gttccaaagc acgcccagcc gtcatagatg gccgctttca cgtgggtcat cccgggatag
     7441 aggtagacgc gaacgccgaa gcgtcgcaat gtgttgatcg ccagggcatt gctgcgccga
     7501 accagctcgc tgtcgggccg cgccggcagg atcacccgca cgtcgacccc gcgtcgccgc
     7561 gctttcgcga gctcgtagat gatctggtca tctcccaggt aagcgttctg cacgaaaatg
     7621 tgcttttccg cccgccggat ggcctcgagc tgcgctcggt aaatctgcgc atcatgaggg
     7681 cgcgtgcgta gcagccgcag ctcctggccg ccctccgcac cgtccgtcct gggggtcctt
     7741 ttggtggagc cgagcagggc cagatcgcca agccggtctt cccgggccgc ctgagcggcc
     7801 tcgctggaca gctcgcccac cacaggcccg gtgagctcga ccatgatgtc gtgccagtcg
     7861 tagcggtact cccggccgat gttcatgccg ccaaggaacg ccacccgctc atcgaagata
     7921 taggtcttcg tgtgatcgcc catgaaccag gtgttgccaa gcatctgcag acgcacgccg
     7981 gaaccttcct tcaggtaggc acccatggac acgggcatga cgtaatcggc cggttgcgag
     8041 tcggccgttg ccaacgtgcc accgatggtg ccgaggccgt ccgccatcac cgtcacggca
     8101 acctcgcgcg atcgccgccg cagcagatcg gcgatggcga ccgcatagtc gtcgttgtcg
     8161 aagatgtagg tccggatctc gatggatcgg cgcgcggccg ctatctcggc gatgagcctg
     8221 tcgaagaacg cttcaccatc caccagaagt ttcatctgcc cccgcgtgcg ttcggttccc
     8281 gtaatccggt ccaggtcgtt ctcccactcc tcgatatcca tgcccctcga cgaggaaagt
     8341 ggacgagccg ccttgcttgc cagcctgggt accaggacga agtcgagagc aagctcctta
     8401 acgatccccg tggctccagt aaacgtggcc gcaagcagat tgcccaccga ggtaagcggc
     8461 cgccgaatga agtcacgagt gtggctgcgc gccacgtggg cggtgagctg catgccgacg
     8521 gcaagccgct gatcgggcgg ttgatccggc gcgagctccc gatccgagtt ggcagccacc
     8581 gatgcctccc ggaacgcctc agacacgcgc accgtatcga ccccggcctg ggcgatttgg
     8641 caaaccagag ctgcggacac cagtgccagc aaataccact tctgcttcac ctcgaattct
     8701 cctcaccgtt cagatttccg aggttcaacg gtagatgccg cgcggcagac ccgcctgagg
     8761 gggcgcgaaa agaatgattt gggtttctta gggttcttta ggccgagcgt cggggggccg
     8821 ccataggaga gcgcaatcaa aaaagcccgc acaaggcggg ccgttttgat
//
LOCUS       DBNHLM010000378         6517 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_836332, whole genome shotgun sequence.
ACCESSION   DBNHLM010000378 DBNHLM010000000
VERSION     DBNHLM010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6517)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_836332"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1549)
                     /locus_tag="AC16QF_17605"
     CDS             complement(101..1549)
                     /locus_tag="AC16QF_17605"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR6450428.1"
                     /translation="MSVSLRQLLEDPDAPEVPIRGLAVDSREVRPGDAFVAISGDRSD
                     GQDYVAEACRNGAVAVLSERPLAAGLELEGCPAVVVPDLKLRRGALAARVHEWPSRRL
                     RCIGVTGTNGKTSIAHFIADLAGRLGRTAGYMGTIGWGRPGALERTRLTTEDGVTVQR
                     RLGQLLDAGCDWVAMEVSSHALSQGRVAEVEFDVAVLSNLSRDHLDYHQTEAAYADAK
                     ASLFRWPSLDTAVVNLDDAFGRRVARSLGPGVALLGYGRQAELGWHDLVFTDQGVRGR
                     WRSPWGAADLALPLAGEFSVANMAAAIGALCCSGLAFDDVVEAAASVAQVPGRMEFLH
                     APGRPAVVVDYAHTPDALDKVLEALRRHTSGALVCVFGCGGDRDPGKRPLMAQAAERR
                     ADVIWMTSDNPRSEDPERIIDDMAAGLRGAACTHRCVDRREAIARAVENASAGDLVVV
                     AGKGHEDYQEVAGRRLSYSDRVEVANALGVNV"
     gene            complement(1546..3198)
                     /locus_tag="AC16QF_17610"
     CDS             complement(1546..3198)
                     /locus_tag="AC16QF_17610"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR6450429.1"
                     /translation="MKGWRHYTLVGGFALGCVAVIVRLGYLNWTEREFLQEQGDARSV
                     RSEVLPAYRGVVYDRHGEPLAVSTPVFAVWLDPSRAGLTDESLGALAELLELDPARLS
                     ASVQSNGHREFLYLKRRVPWDRAQAVRELGISGVHLQSEYRRYYPASETTSHVVGTTG
                     IEDSGLEGIELSFDGRLRGEHGRKIVLKDQRGETIKDLDYVASPKFGDDLNLSLDLRL
                     QFLAYRELKSAVVGHRATSGSLVMLDARSGEILALVNQPSYNPNELGSVEVGNMRNRA
                     VTDVYEPGSTVKPFTVLAALESGRFAADTVIDTGPGYFRVGGKLVQDPVNRGAITVGE
                     ALQVSSQVGIAKIALDLEERAVYDVLSRAGVGEPVNTGLPGEAWASLDDAGLSSLVGR
                     ATLAYGYGLAISPLQLAQAYLTLATGGVRVPVSIVKQEVVRPGTRVFDADMIADVVGM
                     MEGVTDNDGTAPKARVVGYRVAGKTGTARKVGASGYDDERHVALFAGIAPVSDPRLVI
                     VVVVNEPKGDARGGGSVAAPVFGRVAARSMRLLGVRPDGESA"
     gene            complement(3195..3461)
                     /gene="ftsL"
                     /locus_tag="AC16QF_17615"
     CDS             complement(3195..3461)
                     /gene="ftsL"
                     /locus_tag="AC16QF_17615"
                     /inference="COORDINATES: protein motif:HMM:NF016860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HIR6450430.1"
                     /translation="MSRLYGWIVATLLVAVTVSGVQVAFSSQKVRQLHADLQAAQRVQ
                     DEELAEHSRLLLERSAQTSYVNVERVAEAELGMIFPDRVEPIEP"
     gene            complement(3458..4390)
                     /gene="rsmH"
                     /locus_tag="AC16QF_17620"
     CDS             complement(3458..4390)
                     /gene="rsmH"
                     /locus_tag="AC16QF_17620"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR6450431.1"
                     /translation="MSNTHEPVLCQETVATLTGMGNSTVRQGVYVDATFGRGGHSRAL
                     LAVLGDESRVVALDRDPAAVAAGREMASSEPRLTICHARFSDLRRALASLDIESVQGV
                     LMDLGVSSPQLDDPHRGFSFLRSGPLDMRMDPGAGMSAAQWLNSASEQEIAGVLRSLG
                     EERYARRIAAAIAAARPISDTGELVALVRAAQPRATPGKHEATRVFQAVRMHVNDELG
                     ELESGLRQAFEMLSAGGRLAVIGFHSLEDRMVKRFFKSLTEPPPLPRRLPVRAAAQVV
                     PARSVAGPVRAGAAERARNPRSRSAVLRVVEKIS"
     gene            complement(4387..4845)
                     /gene="mraZ"
                     /locus_tag="AC16QF_17625"
     CDS             complement(4387..4845)
                     /gene="mraZ"
                     /locus_tag="AC16QF_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR6450432.1"
                     /translation="MFRGINSATLDGKGRMALPTRHRESVVAVSEGRLVVTIDMREKC
                     LLMYPLPEWEVVQRKLESLSNIGEQARLLQRLLIGHATDLELDGQGRVLLPQMLRGYA
                     ALEKKLVLVGQGNKIEIWSDSLWQSRMNTWLSDGAGELLANGDEFTGLSV"
     gene            complement(5162..5540)
                     /gene="rnpB"
                     /locus_tag="AC16QF_17630"
     ncRNA           complement(5162..5540)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC16QF_17630"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5581..6399)
                     /gene="rsmI"
                     /locus_tag="AC16QF_17635"
     CDS             complement(5581..6399)
                     /gene="rsmI"
                     /locus_tag="AC16QF_17635"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR6450433.1"
                     /translation="MSGVLYVVATPIGNLDDITRRGADVLASVQIVAAEDTRRSQVLL
                     AAIGATQAELVSFHAHNEQNVARRLMAALQAGGDVALVSDAGTPLVSDPGFDLIRRCW
                     EEGVRVVPVPGASSVLAALSVCPLPAGRFFFEGFLPARAGQRRERLRHLAGRTEAVVF
                     FEAPHRVAACLADLEQLVPQRRLMVGREMTKRHESLHCGTPGELREALSSTLRGEFVF
                     VMEAAAGAGLDADAAEVMEILCAELAPAQAARLGSRLLKMDRSVLYRIALEQRS"
BASE COUNT         1162 a         2326 c         2021 g         1008 t
ORIGIN      
        1 agcgccgtca tcacgctcac catggttcgg aatgtcaggt agtggaacac gccgaagccg
       61 ctcacatatc cggacaggta ttcggtcagc cacaacagca tcagacgttc actcccagcg
      121 cgttggcgac ctcgacccgg tcgctgtagc tcaggcggcg cccggccact tcctggtagt
      181 cctcgtggcc tttgcccgcc accaccacca ggtcgcccgc cgaggcgttc tccaccgccc
      241 gggcgatcgc ctcgcggcga tcgacgcagc ggtgcgtgca cgccgcgccc cgcaaccctg
      301 ccgccatgtc gtcgatgatc cgctccggat cttccgaccg ggggttgtcg ctcgtcatcc
      361 agatcacgtc cgcgcgacgc tcggcggcct gggccatgag cggccgcttg cccggatcac
      421 ggtcgccgcc gcaaccaaac acgcacacca gcgcgccgga cgtgtggcgc ctgagcgcct
      481 cgagcacctt gtccagagcg tccggcgtgt gggcgtagtc caccaccacc gcggggcgcc
      541 ccggcgcgtg aagaaactcc atccgaccgg gaacctgggc aaccgaggcc gccgcttcca
      601 ccacgtcgtc gaaggcgagc ccggagcagc acagcgctcc tatggcggcc gccatgttgg
      661 ccaccgagaa ctcgccggcc aggggcaacg ccagatcggc ggcgccccag ggcgaccgcc
      721 agcggccgcg aacgccctgg tcggtgaaga cgagatcgtg ccagccgagc tccgcctggc
      781 ggccgtagcc gagcagggcg acgccgggcc caagagatcg cgccacgcga cggccgaacg
      841 cgtcgtcgag attgaccacc gccgtgtcga gagacggcca ccggaacagg gatgccttgg
      901 catccgcgta ggccgcctcg gtctggtggt agtcgaggtg gtcgcgggac agattggaca
      961 gcacggcgac gtcgaactcc acctcggcaa cccgcccctg actgagggcg tgggagctga
     1021 cttccatggc aacccaatcg cagcccgcat ccagaagctg gcccagacgg cgctgcacgg
     1081 tcaccccatc ctcggtggtg agcctcgtgc gttccaacgc cccgggcctg ccccagccga
     1141 tggtgcccat gtagccggcg gtccggccga ggcgtccggc cagatcggcg atgaagtggg
     1201 caatggacgt cttgccgttt gtccccgtca ccccgataca cctcagccgg cggctcggcc
     1261 actcgtgcac gcgagctgcc agggcgcccc ggcgcagctt gagatcgggc acgaccacgg
     1321 ccgggcagcc ttccagctcc agccccgccg ccagcggtcg ctccgacagc acggccaccg
     1381 cgccgttacg gcaggcttcg gcgacgtaat cctgcccgtc cgaacgatcc ccggagatcg
     1441 ccacgaacgc atcccccgga cgaacctccc gggagtccac cgccagcccc ctgatcggca
     1501 cctcgggggc gtcggggtct tcgagcagct gccgcaggga tacgctcacg ctgactcccc
     1561 gtccgggcgt acgcccagaa gacgcatgga gcgcgcggcc acccgaccaa acaccggcgc
     1621 ggcaacgctg ccgccgccgc gggcatcgcc cttgggttcg ttgaccacca cgacgatgac
     1681 cagtcgcgga tccgataccg gcgctattcc ggcaaacagc gcgacgtggc gctcgtcgtc
     1741 gtagcccgag gcgcccacct tgcgggcagt gcccgtcttg cctgccaccc ggtagcccac
     1801 cacgcgggcc ttgggcgcgg tgccgtcgtt gtcggtgacg ccctccatca tcccgacgac
     1861 gtccgcaatc atgtccgcat cgaacacgcg cgtgcccgga cgcacgactt cctgcttcac
     1921 gatggacacc ggcacccgga cgccaccggt tgcaagcgtg aggtaggctt gtgcgagctg
     1981 caggggcgag atggcgagac cgtaaccgta ggccagcgtg gcacggccca ccaggctgga
     2041 gaggcccgcg tcgtcgagcg acgcccaggc ctcgcccggc aggccggtat tgacgggctc
     2101 acccacaccg gcacgactga gaacgtcgta cacggcccgc tcctccagat ccagcgcgat
     2161 cttcgcgata cccacctggc tggatacctg cagcgcttcg ccgacggtga tggcaccccg
     2221 gttgaccggg tcctgaacca gcttgccgcc aacccggaag taacccggcc cggtatcgat
     2281 gaccgtatcg gcggcgaagc gaccggactc gagggcggcg agcaccgtga acggctttac
     2341 ggtggacccg ggctcgtaca cgtccgtgac cgcgcggttg cgcatgttcc ccacttccac
     2401 cgagccgagc tcgttggggt tgtaggacgg ctgattgacc agggcgagaa tctcgccgct
     2461 gcgcgcatcc agcatgacga gggagcccga cgtggcacga tgacccacca ccgcggactt
     2521 gagctcccga taggcgagaa actgcagcct cagatcgagg ctcaggttca ggtcgtcgcc
     2581 gaatttcggc gaggcgacgt aatcgagatc cttgatggtc tcgccgcgct gatccttcag
     2641 gacgatcttc cgtccgtgct cgccgcgaag ccggccgtcg aaggagagct cgatcccctc
     2701 cagcccggaa tcctcgatgc cggtggtgcc gaccacgtga gacgtcgtct cgctggccgg
     2761 gtagtagcgg cgatactcgg actgcaggtg aacgcccgag atgcccagct ccctgacggc
     2821 ctgcgcccgg tcccagggga cccggcgctt gaggtagaga aactcccgat ggccgttgct
     2881 ctgcacggac gcggacaggc gagcggggtc cagttcgagc aactcggcaa gcgcgcccag
     2941 cgactcgtcc gtcaggccag cacggcttgg atccaaccag accgcgaaaa cgggggtgct
     3001 gactgccagc ggctcgccat ggcggtcgta gacgacaccg cgataggccg gcagcacctc
     3061 gctgcgaacg gatctggcat cgccctgctc ctgcaggaac tcccgttcgg tccagttgag
     3121 gtaaccgagc cgaacgatca cggccacgca gcccagagcg aagcctccca ccagcgtgta
     3181 gtgcctccag cccttcatgg ctcgatgggc tccacccggt cggggaagat catgccaagc
     3241 tcggcttccg ccacccgttc gacgttcacg tatgaggtct gcgcgctgcg ctccaggagc
     3301 agccggctgt gctcggcgag ttcctcgtcc tgaacacgct gcgcggcctg aagatcggcg
     3361 tgaagctgcc gcactttctg gctgctgaac gcaacctgca cgccggacac cgtgaccgcg
     3421 accagcaggg ttgccacgat ccagccgtaa agccgactca tgagattttc tccaccaccc
     3481 gaaggacggc gctgcgcgag cgcgggttgc gcgcccgttc ggcagcgcca gcgcggacgg
     3541 gccctgccac cgagcgcgcc gggacgacct gagccgccgc ccttaccggt aaccggcgcg
     3601 gcaacggcgg gggctccgtt aagctcttga agaagcgctt gaccatccgg tcttccagag
     3661 agtgaaagcc gatcaccgcc aggcgccccc cggcagatag catctcgaat gcctgtcgca
     3721 acccgctctc gagctcgcca agctcatcgt tgacgtgcat ccgcaccgcc tggaacacgc
     3781 gggtggcctc atgcttgccg ggtgtggctc gcggctgcgc cgcgcgcacc agcgccacca
     3841 gctcaccggt atcggagatt ggccgggccg ccgcgatggc ggcggcgatg cgcctggcat
     3901 agcgctcctc tccgagcgat cgcagcacgc cggcgatctc ctgctccgaa gccgaattga
     3961 gccactgcgc ggcagacatt cccgcgcccg ggtccatgcg catgtccagg ggcccggacc
     4021 gcagaaagct gaatcctcga tgaggatcat ccagctgggg agaggagacg ccgagatcca
     4081 tgagcacgcc ctgcaccgac tcgatgtcga gcgaggcaag cgcccggcgc agatcagaaa
     4141 agcgcgcgtg acaaatggtc aaacggggct cggagctggc catctccctt cccgccgcga
     4201 cggccgccgg gtcccgatcc agggcaacca cccggctctc gtcaccaaga accgccagga
     4261 gcgcgcgcga atggccaccg cgcccgaacg tggcgtcgac gtacacgccc tgcctgactg
     4321 tcgagttccc catccccgtc aatgtcgcca ccgtctcctg gcaaagcacc ggctcgtgcg
     4381 tattgctcaa accgacaatc cggtgaactc gtcgccgttg gccagcagtt ccccagcccc
     4441 atccgagagc caggtgttca tccggctctg ccacagcgaa tcgctccaaa tttctatctt
     4501 gttgccctga ccgaccagca ccagcttctt ttccaatgcc gcatagcctc tgagcatctg
     4561 cggcagcagc acccgaccct ggccgtccag ctcgaggtcc gtggcatggc cgatcagcag
     4621 ccgctgcagc aaccgggcct gctcaccgat gttcgaaagg ctttcgagct tgcgctgcac
     4681 cacctcccat tccggaagcg ggtacatcag caggcacttt tcccgcatgt cgatggtcac
     4741 gaccaggcgc ccctcgctca cggcgaccac cgactcgcga tgacgggtcg gcagagccat
     4801 gcgccccttg ccatccagag tggcgctgtt gatgcctcga aacatgactg ccctgggggc
     4861 cgcgcgaatc ggtcaaaatg aaccaggtca cgaccgattc agacggaatc tcccacaatc
     4921 tcccactaaa tcccaaattg gcacaccacc agccacgata tcgacccaca ggcttgcctg
     4981 tcaagcgagg aaaacgtcga taacctacgg aaatttatgg attttttcgg cgcagagagg
     5041 ctgtcagaag gcagcgggca gctttatctt cttagttaac ctcaggtaga agcaggtatc
     5101 tagaattcac cttaacttct agacccaatc ggcattaaaa atcccgtttt aggtgcgaaa
     5161 agaaacgagt cggccgataa gccgggttct gttgccgcgt ccgagaacgc agccgacagt
     5221 cattcatcta gggcgttcgt caccgaacgc ctcgtgcgac ctacccgaac ccggtgcgga
     5281 ccacaccatg gggttcctat ttggccttgc tccaggcggg gtttacctag ccgcgcagtg
     5341 ttaccacgcg cgcggtgcgc tcttaccgca ccctttcacc cttaccgttc gcagcgccgg
     5401 agcgccgcgc cttaggcggt ttgctttctg ctgcactttc cgtgggctcg cgccccccag
     5461 gcgttacctg gcacctcgtc ctgtggagcc cggactttcc tctcgcagct gatgctgcca
     5521 gcgactgtct ggccgactcg agggcaagac taatccccgg accggcggat tgccaggccc
     5581 tcaggagcgc tgctccaggg cgatccggta gagcacggag cggtccatct tgagcaggcg
     5641 cgagccgagc cgtgcggctt gagccggagc cagctcggca caaaggatct ccatgacctc
     5701 ggcggcgtcc gcgtcgaggc cagctcccgc ggcggcctcc atcacgaaga cgaactcccc
     5761 ccgaagggtg ctcgacagcg cctcgcgcag ctctcccggg gtgccgcagt gcagactctc
     5821 gtgacgtttg gtcatttccc gcccgaccat gagtcgccgc tgcgggacca gctgctccag
     5881 atccgcaagg caggcggcga cccggtgggg cgcctcgaag aagacgacgg cttccgtgcg
     5941 tccggcaaga tgccgcagcc gctcgcggcg ctgccccgcc cttgccggca gaaagccctc
     6001 gaagaaaaag cggccggccg gaagaggaca gacggacaga gcagccagga ccgagctcgc
     6061 gccggggacg ggaaccaccc ggacgccttc ctcccagcag cgccggatga gatcgaagcc
     6121 cgggtcggat accaggggcg tgccggcgtc ggagaccagt gccacatcgc cacccgcctg
     6181 cagcgcggcc atgagacgcc gcgccacgtt ctgctcgttg tgcgcgtgga acgagaccag
     6241 ctcggcctgg gttgcgccga ttgctgctaa cagcacctga gagcgtctgg tatcctcggc
     6301 ggcgactatc tgcaccgacg ccaggacatc ggcgcctcga cgagtgatgt cgtcgaggtt
     6361 tccaattggc gtggcaacga cgtagagaac accagacatg gcagcaaacc ggataagggc
     6421 ctgggcttgg gcgttcgcta ttctcgcagt gtccgcctgt caaagcacgc cgctcgagac
     6481 aatcgaagcg aacgtgcccg aagtctcccc ctcatct
//
LOCUS       DBNHLM010000379         5685 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_717066, whole genome shotgun sequence.
ACCESSION   DBNHLM010000379 DBNHLM010000000
VERSION     DBNHLM010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_717066"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="AC16QF_17640"
     CDS             complement(<1..594)
                     /locus_tag="AC16QF_17640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6450434.1"
                     /translation="MADWDERKRRARESYQRFVPDADPERVLASMERSLGALGTFAFA
                     AVGEMWDRPELSRRDRSLMVIAVLAAQARDEELTLHTRIGMRHGLSRTEIEEINLHVA
                     AYAGFPAAMAAQRRMDQAFREAQGVEHIDDREGAEHLGDDERLRRAADVRRTLAGAEA
                     ALDPEADLASLRDYLGGVGEWAMKWAFGEVWSRPQLSR"
     gene            complement(623..865)
                     /locus_tag="AC16QF_17645"
     CDS             complement(623..865)
                     /locus_tag="AC16QF_17645"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR6450435.1"
                     /translation="MLVSLNGALRAAAGGQDSVNIEASTIRQLIERLVDRYPAMAGEV
                     DKGIAVAIDGVVYRDDWTQRIPENAEVFLMPRIAGG"
     gene            complement(855..3140)
                     /locus_tag="AC16QF_17650"
     CDS             complement(855..3140)
                     /locus_tag="AC16QF_17650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6450436.1"
                     /translation="MAHDNSYTGQKFQVIGNRFARPDGVDKVTGRARYGADANAPGQL
                     VARVLRSPHAHARIKKIDTSKAEKLSGVKAVITSDDLPDLTGGNRGMTDILENCMARG
                     RALYDGHAVAAVAAIDEQTARKALKLIKVTYQMLPHVTDVDEAMQPEAPVIHDWVFTG
                     GMEPKPTKPSNVANRTEMGHGDVDAGFAAADVIVERSYKTEQTHQGYIEPHACLASVN
                     PDGSAELWVTTQGPFSYRNVCAGLLGLDIAKLKVTASEIGGGFGGKTHVWAEPLALAL
                     SRKANRPVKLVMTREEVFRATGPTASTSIDVKIGAKKNGEITAATLDMRYQGGAFPGI
                     WGMLGCMTGFACYDIKNQRSVGWDVIVNRPKVATYRAPSAPMAAFAVESTMDIVAGEV
                     GMDAVDFRLKNVAREGSQAPYGPIFGPIGIESALQEVKKHPQMKARLKKNQGRGVACG
                     FWFNIGGQTCVDLNIGEDGTVNLAVGTVDVGGARSSLGMIAAEELGISYDDVKVHVAD
                     TASLGHNDTTEGSRGTFSSGMAAIFAARDAIEVLKDRAAQMLDVPRDAIVWEKGEARA
                     KGTQYSNLPKLSLREIAAKAARTGGPIAGHNEVTADGAGVAFAAHICDVEVDPETGLT
                     RVLRYTVIQDAGKAIHPDYVEGQFQGAAAQGIGWGLNEEYVYGEDGRLQNPGFLDYRI
                     PVCSDLPFIDTKILEVPNPNHPYGIRGVGEASIVPPLGAIGNAIANATGVRMQHVPMS
                     PVRVLDALDQAEASESQVSAG"
     gene            complement(3168..3656)
                     /locus_tag="AC16QF_17655"
     CDS             complement(3168..3656)
                     /locus_tag="AC16QF_17655"
                     /inference="COORDINATES: protein motif:HMM:NF013923.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR6450437.1"
                     /translation="MSNAVLVSTTINGDTVEFACPADEVLLDSLRDRVGLTGVKEGCG
                     TGDCGACSVTLDGELVCSCLVLGVEADGRAIGTVEGLTENGDLHPLQQNFIDHAALQC
                     GICTPGFLVAAKALLDRNPNPTETEIRYALAGNLYRCTGYDKIVRAVQASAKDLRKAK
                     KR"
     gene            complement(3653..4513)
                     /locus_tag="AC16QF_17660"
     CDS             complement(3653..4513)
                     /locus_tag="AC16QF_17660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR6450438.1"
                     /translation="MRYESPASTKEAVALLAQEKGSAFVLAGGTDLLVRMKAELIEPD
                     LVVDIKRIPAMTDIRVTEKGFRIGAAVSAAQLGRHKALCKAWPGVVEAANLIGSDQIQ
                     GRCTVVGNLCNASPAADSVPALVAAGAKAVIVGRNGRRTVPVEKVITGPGRTSLEKGE
                     VIEALTLPKPASRTGDAYLRFTPRTEMDIAVVGVGISLALERGTVKKARVCLGAVAPT
                     PILVPAAARALIGSKLDDVALARLATACSAACNPIDDKRGTAAYRIRVAGVLARRAAT
                     IAFQRAGGKA"
     gene            4812..>5685
                     /locus_tag="AC16QF_17665"
     CDS             4812..>5685
                     /locus_tag="AC16QF_17665"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6450439.1"
                     /translation="MPQREVIEGLYSGATYSPYAERSFPSEVFWGETHLHTGLSLDAG
                     LFGNILGHEDAYRLARGEQITSSSGLPIKLGRPLDWLVIADHSDMMGIATDIQRGAPN
                     IRAVPKGREWSDGFKQGGQAAAEAAFDLIQHFAQFKVPPELVEQYSPGSPIYSGVWDS
                     IIETADRFNDPGRFTAFIGFEWTSVPKGFNLHRNVVLRDDGRRASQVMPLTTQPPLGT
                     TDPLDLYQWLEDYESKTGGQALALAHNGNLSNGWMFPMGKTYAGGRVNKEYVQLRAKW
                     EPLYEVTQIKGDGEA"
BASE COUNT          908 a         1895 c         1796 g         1086 t
ORIGIN      
        1 gcgggagagc tgcggccgcg accagacctc cccgaacgcc catttcatcg cccactcgcc
       61 aacgccgccg agatagtcgc gcagcgacgc cagatcggct tccggatcga gggcagcctc
      121 ggcgccggcg agcgtgcgcc gcacgtcggc cgcccgacgc agccgctcgt cgtcgccgag
      181 atgctccgct ccctcgcggt cgtcgatgtg ttccacgcct tgggcttcgc gaaaagcctg
      241 atccatacgc cgctgtgcgg ccatggccgc aggaaagccc gcgtaggcgg cgacgtggag
      301 gttgatctcc tcgatctcgg tgcggctgag cccgtgtcgc atcccgatgc gggtatgcag
      361 agtcagctcc tcgtcgcggg cctgggccgc cagcacggcg atgaccatga ggctgcgatc
      421 ccggcggctg agctcgggcc ggtcccacat ctcgccgacc gcagcaaaag caaacgttcc
      481 cagcgcgccg aggctgcgct ccatggatgc cagcacgcgc tcggggtctg cgtccggtac
      541 gaagcgttgg tacgattccc tcgcgcgccg tttgcgctcg tcccagtcag ccatggctta
      601 cctctgctcg gttttttctg gctcaaccac ccgcaatgcg cggcatgaga aagacctcgg
      661 cgttctccgg aatccgctgg gtccagtcgt cacggtagac cacgccgtcg atggcgacgg
      721 cgatgccttt gtccacctct ccggccatgg cgggatagcg atccaccaac ctctcgatca
      781 gctgccgaat ggttgacgcc tcgatgttca ccgagtcctg gcccccggcg gcagcacgca
      841 gcgccccgtt gaggctaacc agcactgacc tggctttccg acgcctctgc ctgatccagg
      901 gcgtcgagca cgcgtaccgg cgacatcggc acgtgctgca tgcgcacacc ggttgcgttg
      961 gcgatcgcgt tcccgatggc gcccagcggt ggaacgatgg acgcttcacc caccccacgg
     1021 ataccgtagg gatggttggg gttcgggact tccagaatct tcgtgtcgat gaagggcagg
     1081 tctgagcaga cggggatccg gtagtccagg aagcccggat tctgcaggcg accatcttcg
     1141 ccgtagacgt attcctcgtt tagcccccag ccaatgccct gggccgcggc gccctggaac
     1201 tggccctcca cgtagtcggg atgaatggcc ttgcccgcgt cctgaatgac cgtgtaccgc
     1261 agcacacggg tcagtccggt ctcgggatcc acctcgacgt cgcagatgtg agcggcgaaa
     1321 gcgacacccg ccccgtccgc cgtcacctcg ttgtggcccg cgatggggcc acccgtgcgt
     1381 gcggccttgg cggcgatctc cctgagagag agcttgggca ggttgctgta ttgggtaccc
     1441 tttgcacgcg cctcgccctt ctcccagacg atggcgtccc ggggcacgtc cagcatctgc
     1501 gcggcgcgat ccttcaacac ctcgatggcg tcccgggcgg caaatatagc cgccatgccc
     1561 gaggagaagg tgccccggct gccttccgtg gtgtcgttgt ggccaaggga tgcggtgtcg
     1621 gccacgtgca ccttgacgtc gtcataggaa attcccagtt cctccgcggc gatcattccc
     1681 agggacgagc gcgcgccgcc cacgtctacg gttccaactg ccagattgac ggtgccgtct
     1741 tctccgatgt tgagatccac acaggtctga ccaccgatgt tgaaccagaa gccgcaggcg
     1801 acgccccttc cctggttctt cttcaggcgg gccttcatct gcgggtgttt cttcacctcc
     1861 tgcaacgcgg actcgatgcc gatgggaccg aagatcggac cgtaaggtgc ctgcgagccc
     1921 tccctggcga cgtttttgag acggaaatcc accgcatcca tgccgacctc gccggctacg
     1981 atgtccatgg tgctttccac cgcgaacgcc gccatgggtg ccgagggcgc ccggtaggtg
     2041 gccaccttcg gccggttgac gatgacgtcc cagcccacgg atcgctggtt cttgatgtcg
     2101 taacaggcga aaccggtcat gcatcccagc atgccccaga tgccgggaaa cgcgccgccc
     2161 tgatagcgca tgtccagggt cgctgcggtg atctcgccgt tcttcttggc gccgatcttc
     2221 acgtcgatgg acgtggacgc cgttgggccg gtggcgcgaa agacctcttc gcgggtcatc
     2281 accagcttga cgggccggtt ggccttgcgg gaaagggcca gagccagggg ctcagcccag
     2341 acgtgggtct taccgccgaa gccgccgccg atctccgagg ccgtcacctt cagcttggcg
     2401 atgtccaggc ccagcaggcc ggcgcacacg ttccggtacg agaacggtcc ctgggtggtt
     2461 acccacagct ccgccgagcc atccgggttg accgatgcca ggcaagcgtg gggctcgatg
     2521 tagccctggt gggtctgctc ggttttgtac gaccgctcca cgatcacgtc ggctgccgcg
     2581 aacccggcat ccacgtcgcc gtggcccatt tccgtgcggt tggcgacgtt cgagggtttt
     2641 gtcggcttgg gctccatgcc gccggtgaac acccagtcgt gaatcacggg cgcctccggt
     2701 tgcatcgctt cgtccacgtc ggtgacgtgg ggaagcatct gataggtcac cttgatcagc
     2761 ttcagcgcct tgcgggccgt ctgctcgtct atggcggcca ccgcggccac tgcgtgaccg
     2821 tcgtagagcg cccgcccgcg ggccatgcag ttctccagga tgtccgtcat cccgcgattc
     2881 ccgccggtca ggtccggcag atcgtcgctc gtgatgacgg ctttgacgcc ggataatttc
     2941 tccgccttgg acgtgtcgat cttcttgatc ctggcgtggg catgaggtga gcggagaacc
     3001 cgggcgacca gttgacccgg tgcattggcg tccgcaccgt aacgagcccg gccggttacc
     3061 ttgtcgacgc cgtcgggccg ggcgaaccgg ttgccgatca cctggaattt ctgtccggtg
     3121 tagctgttgt cgtgggccat gtcgaattcc tatcgtcgcg tcagtggtta acgtttcttg
     3181 gctttgcgca gatccttggc ggaggcctgc accgcgcgaa cgatcttgtc gtagccggtg
     3241 cagcggtaca gattgcccgc cagggcatag cgaatttccg tctccgtcgg gttcggattt
     3301 ctgtctagca gcgctttcgc cgcgacgagg aaccccggag tgcagatgcc gcactgcaga
     3361 gcggcatgat cgatgaagtt ctgctgtaag gggtgcaggt cgccgttctc ggtgagacct
     3421 tccaccgtgc cgatggcgcg gccgtcggct tccacgccga gcaccaggca ggagcacacc
     3481 agctcaccgt ccagcgtcac gctgcaggca ccgcagtcgc ccgtcccgca gccctccttg
     3541 acgccggtca gaccgacccg gtcgcgcagg gagtccagca gcacctcgtc tgccgggcag
     3601 gcgaactcga ccgtgtcgcc gttgatggtc gtggatacta aaaccgcgtt gctcatgcct
     3661 tacctcctgc gcgttgaaac gcgatcgtcg ccgcgcgccg cgccagcact cccgccacgc
     3721 ggatgcggta agccgccgtg ccgcgtttgt cgtcgatggg gttgcaggcc gcggagcatg
     3781 ctgtggccag cctggccagc gcgacgtcgt cgagcttgga tccgatcagt gctcttgccg
     3841 ccgccggcac cagaatcggc gtgggcgcca ccgcccccag gcagacgcgc gcttttttga
     3901 ccgtgcctcg ctccagcgcc aggctgatcc cgacgcccac cacggcgata tccatctcgg
     3961 ttcgaggggt gaaacgcaag taggcgtcgc ccgtgcggct ggccggtttc gggagcgtga
     4021 gggcttcgat gacctcgcct ttttccaggg aggttctgcc cggcccggtg atcaccttct
     4081 ccaccggtac cgttcttcgt ccgttccggc ccacgatgac ggcctttgcc ccggcggcga
     4141 ccagggccgg cacgctgtcg gccgcgggcg atgcgttgca caggtttccg accaccgtgc
     4201 agcgcccctg aatctgatcc gagccgatga gattggccgc ctccaccacg cccggccacg
     4261 ccttgcacag ggctttgtgc cggccgagct gggcagcgga aacggccgct ccgatccgaa
     4321 accccttctc ggtcacccgg atgtcggtca ttgctggaat gcgtttgatg tcgacaacga
     4381 ggtcgggttc gatcagctcg gccttcatcc tgaccagcag gtcggtgccg cccgccagca
     4441 cgaaggctga gcctttttcc tgggccagca gcgcgacggc ttctttcgtc gaggctgggg
     4501 attcgtatct catcgtgagt gtcctgtggc tgaagtcggt gagggatcag acgatctccg
     4561 catcctatcc gcggcgccgc cttccggcaa cgatctcgtc ttcgagcgaa gctgattgac
     4621 cgggatcgat tcattcgtcc acacgaatcg tctaccattc gagcatcggg ggaaaacgtc
     4681 agtcggcgcg cgctggctgc aggctgaaaa aaatttaaca cgccctcgat gaggagtccg
     4741 gcatgacacg tcctatcgca ctgtatttgt tccttctggc acctctgacg gcatcggcct
     4801 cggacatcgg aatgccgcaa agggaggtca tcgaagggct ctactctggg gccacatact
     4861 cgccttacgc cgagcgctcg ttcccgagcg aggtcttctg gggagagacc caccttcaca
     4921 ccggcctgtc tctcgacgct gggctgttcg gaaacattct gggccacgaa gacgcttatc
     4981 gcctggctcg aggcgagcag atcacgtcct cgagtggtct gcccatcaag ctgggccgac
     5041 ccctcgactg gctggtgatc gccgaccact ccgacatgat gggcatcgcc accgacattc
     5101 agcgaggcgc ccccaacatt cgcgcggtgc cgaaaggccg cgagtggagc gacgggttca
     5161 agcagggggg gcaggcggct gccgaggccg cgttcgatct cattcagcac tttgcgcagt
     5221 tcaaggttcc cccggagctg gtcgagcagt attcccctgg ttcgcccatc tattccggcg
     5281 tgtgggacag catcatcgag acggccgacc gcttcaacga tcccgggcgt ttcaccgctt
     5341 tcatcgggtt cgaatggacg tcggttccca agggtttcaa cctgcatcgc aacgtggtgc
     5401 tgcgagacga cggccgacgg gccagtcagg tgatgcccct gaccacccag ccgccgttgg
     5461 ggaccaccga ccctctggat ctctaccagt ggttggagga ctacgagagc aaaaccggcg
     5521 gtcaggcgct ggctctggcg cacaacggga acctgtccaa cggttggatg tttcccatgg
     5581 gcaagacata cgcggggggc cgcgtgaaca aggagtacgt gcagctgcgc gcgaagtggg
     5641 agccgcttta cgaggtcacc cagatcaagg gagacggcga agcgc
//
LOCUS       DBNHLM010000380         3535 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_598051, whole genome shotgun sequence.
ACCESSION   DBNHLM010000380 DBNHLM010000000
VERSION     DBNHLM010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_598051"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="AC16QF_17670"
     CDS             complement(<1..207)
                     /locus_tag="AC16QF_17670"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR6450440.1"
                     /translation="MLLCLDVGNSQIYCGVYDGDRLTVQFRRTSTVRSSSDELGVFLR
                     GALRENGVSPDGITQIAICSVVPDV"
     gene            complement(222..998)
                     /gene="panC"
                     /locus_tag="AC16QF_17675"
     CDS             complement(222..998)
                     /gene="panC"
                     /locus_tag="AC16QF_17675"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR6450441.1"
                     /translation="MKVLSSLGDWRAFRAGLVGEIGFVPTMGALHEGHAQLLQRSREA
                     NDHTVVSIYVNPTQFNNPADLDSYPGTLGEDLCRARELGVNAVLIPSYDELYPDDFRF
                     RVEEHEFSRELCGAHRPGHFTGVMTVVLKLLNLVRPRRAYFGEKDYQQYLLIRDMCDA
                     FFVDVEIVPCATVREEDGLALSSRNALLDEKGRARAGRLNRLLRDAPSDAAASRALEQ
                     AGFEVDYVVTKADRRFAAATLRCGEREVRLIDNVAIAQHG"
     gene            complement(995..1783)
                     /gene="panB"
                     /locus_tag="AC16QF_17680"
     CDS             complement(995..1783)
                     /gene="panB"
                     /locus_tag="AC16QF_17680"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR6450442.1"
                     /translation="MNVLDFPARKLNREKISMVTCYDFWSAQILEQTEVDCLLVGDSL
                     AMVMHGFDSTVHADTALMALHVAAVRRGAPSKFIVADMPFLAARKGLQPAMDAVQTLM
                     QAGANAVKIEGEAGQAELMTHIVQSGVPVMGHLGLTPQSVHTIGGHKVQGRTDEAAEQ
                     LRSSAEALAAAGCFALVLECVPSRLAAKVTRAIDIPTIGIGAGPETDGQVLVLQDLLG
                     MNPDFKPKFLRHYANGFESVSGAVNRFHADTQRGCFPSKSESYA"
     gene            complement(2072..3415)
                     /locus_tag="AC16QF_17685"
     CDS             complement(2072..3415)
                     /locus_tag="AC16QF_17685"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR6450443.1"
                     /translation="MMWNDSESEIFAQMAGRLAAAFADLPAFSAGLLDRDAVEVLRET
                     AQRLADNYPYFHPLYAGQMLKPPHPVARLAYGLAMLINPNNHALDGGRASSEMERAAV
                     AELAAMFGWQTHLGHLTSGGTMANLEALWIAGALQPGGVVLASEQAHYTHERLSGVLR
                     LPFEPIAADASGKMRMDALEDRLRRGGVGTVVATVGTTGFGAVDPLADIVELRHRYGF
                     RLHADAAYGGYFVLAGNLQAETRRHFDAMGAVDSIVVDPHKHGLQPYGCGSVLFRDPA
                     VGAFYKHDSPYTYFTSDALHLGEISLECSRAGAAAVALWATHRLLPPRPGGEFARGLE
                     RCRDAAEALHGRLAGHHRFLPLVQPELDIVAWAVLGADAEEASAAGRRLFEEAGELGL
                     HLALVQAPGSMIAHRWPEGADIPEGPVTCLRSVLMKPEHLDWLDRIWSLLEDLAG"
BASE COUNT          669 a         1203 c         1075 g          588 t
ORIGIN      
        1 aacgtcgggc acgacggagc agatggcgat ctgagtgatc ccgtcgggtg acacgccgtt
       61 ttctcgcagc gcgccgcgca gaaacactcc gagctcgtcc gaggagctac gcaccgtaga
      121 ggttcgtcga aactgcacgg tgaggcggtc gccatcgtag acgccgcagt agatctggct
      181 gttacccaca tccaaacaga gcagcatgcc tcctcctcgg cctagccgtg ctgcgcgatc
      241 gcaacgttgt cgatcagtcg gacttctctt tcgccgcagc gcaaagtggc ggcggcgaat
      301 cgccgatcgg ctttggtgac cacgtagtcc acctcgaatc ccgcctgctc gagagcccgg
      361 gaagcagcag catcgctcgg cgcgtcgcgc agcagccgat tcagccgacc agcacgcgcg
      421 cggcctttct cgtccagcaa cgcgtttcgg gaactcagcg ccagcccgtc ctcctcgcgt
      481 accgtggcac atggcacgat ttcgacatcc acgaagaacg catcgcacat gtcacgtatc
      541 agaagatact gctgataatc cttttcgccg aagtacgcgc gccgcggccg gacaaggttg
      601 aggagcttga gcaccacggt catcacgcca gtgaagtgcc ccggacgatg cgccccgcaa
      661 agctcacggc tgaactcgtg ttcctcgact cgaaaccgga agtcatcggg atagagctcg
      721 tcgtagctcg gtatcagcac cgcgttcacg ccgagctccc gggcgcggca aaggtcctcg
      781 ccgagcgtcc cagggtagct gtcgagatcg gcgggattgt tgaactgagt ggggttcaca
      841 tagatgctca caaccgtgtg gtcgttcgct tcccgactgc gctggagcag ctgggcatga
      901 ccttcatgga gcgcgcccat ggtcggcacg aacccgatct cgccgacgag cccggcgcgg
      961 aacgcccgcc aatccccgag gctggagagg actttcacgc gtaggactcc gatttcgacg
     1021 gaaagcagcc gcgctgggtg tcggcgtgaa accggttgac ggcgccgctc accgactcga
     1081 acccgttggc gtaatgacgc agaaacttgg gcttgaaatc gggattcatg ccgagcagat
     1141 cctgcagaac gagcacctgc ccatccgttt ccggtccggc tccaataccg atcgtcggaa
     1201 tgtcgatggc ccgggtgacc tttgcggcga gccgcgaggg cacgcactcg agaaccagcg
     1261 caaagcaacc ggccgcggcc agcgcctcgg cagaggaccg cagctgctcg gcggcctcgt
     1321 cggtccggcc ctgcaccttg tgcccgccga tggtgtgtac cgactgaggg gtgagcccga
     1381 ggtgtcccat gaccggaacg cccgactgca cgatgtgcgt catcagctcg gcctgccccg
     1441 cttcgccctc gatcttcacg gcgtttgcgc cggcctgcat cagcgtctgg acggcgtcca
     1501 tcgcgggctg cagccccttg cgggcggcaa gaaacggcat gtcggcgacg atgaatttcg
     1561 acggcgcgcc ccgacgcacg gcggcgacgt gcaacgccat cagcgcggta tccgcatgca
     1621 ccgtcgagtc gaatccgtgc atgaccatcg ccaggctgtc accgaccagc agacaatcca
     1681 cctcggtctg ctccaggatc tgagcagacc agaagtcata acaggtgacc atcgagattt
     1741 tttctcgatt aagtttgcgc gcgggaaaat caagcacatt cactgcgggg ccctccggtt
     1801 gcgatcaccg atgcagtacc cgcacgagcg gcgcacgccg ttctgcttca ggtacctgtc
     1861 ctttcatcgg gatcaagtcc aattgcctat ggcggttcaa atctctgcct ggcgccgacg
     1921 tcaatgcggg gcgccacggt ctccgtgctg agcatcggag ccgcagaaaa ccctacgtca
     1981 ctctcacagg aaagtccatc cgctccggtg aacgcgcaac gggcgcaaaa tgagacccaa
     2041 aacccctttc tccttccgcg tgaatggagt ttcagccggc cagatcctcc agcagtgacc
     2101 agattcggtc cagccaatcc agatgttccg gcttcatgag caccgaccgc agacaggtca
     2161 ccggcccctc gggaatgtcc gcgccctcgg gccagcggtg agcaatcatc gaccccggcg
     2221 cctggaccag cgccaggtgc agcccgagct caccggcttc ctcaaacagg cgccggcccg
     2281 ccgcgctggc ctcctcggca tctgccccca acaccgccca tgcgacgatg tcgagctcgg
     2341 gctgaacgag gggcaggaac ctgtggtgac cggcgagtcg gccgtggaga gcttcagcgg
     2401 cgtcgcgaca gcgctcgagc ccccgggcga actcgccacc cggcctcggg ggcagcaacc
     2461 gatgggttgc ccacagcgcg acggccgccg cccccgcccg cgagcattcc aggcttatct
     2521 ccccgagatg cagcgcgtcg gaggtgaagt acgtgtaagg cgagtcatgc ttgtaaaacg
     2581 cgcccaccgc cggatcgcga aacagcacgc tgccgcagcc gtaaggctgc agtccatgct
     2641 tgtgcggatc caccacgatg gaatcgactg cacccatggc atcgaaatgc cgacgggtct
     2701 ctgcctgaag gttgccggcg agaacgaaat agccaccgta agcggcatcg gcgtgcagcc
     2761 gaaacccata gcggtgccgt aattcaacga tgtcggcgag aggatctacg gctccgaagc
     2821 ccgtggtgcc aacggtagcg accaccgtac ccacgccacc gcggcggagc cgatcctcca
     2881 gcgcatccat ccgcatcttc cccgaggcat ctgcggcgat cggctcgaac ggcagcctca
     2941 gcacgccgct caggcgctca tgggtatagt gcgcctgctc cgaggcgagc acgaccccac
     3001 cgggctgcag cgcgccggcg atccacagcg cttccaggtt ggccatcgtt ccgccgctgg
     3061 tgagatgtcc gagatgggtc tgccagccga acatggctgc cagttccgcc acggccgccc
     3121 gctccatctc ggagctggcg cggcctccat ccagcgcgtg gttgttcgga ttgatgagca
     3181 tggcaagacc gtatgcgagc cgggccaccg gatgcggcgg cttcagcatc tgccccgcgt
     3241 acagcgggtg gaagtacggg tagttgtctg cgagacgctg cgcggtctcc cgcagaacct
     3301 cgaccgcatc acggtcgagg agcccggcgc tgaaggctgg aaggtcggca aatgctgcag
     3361 cgagtcgacc agccatctgc gcgaatatct ccgactcgga gtcgttccac atcatcagcc
     3421 tagctctcca ttgccctgct catgccgccg gatcagcatg ggcgagacca cctaaggatg
     3481 agggccctta gcgctcgatc ccccgacagc tgcccctcat cgacctcgga cgatc
//
LOCUS       DBNHLM010000381         6123 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1137237, whole genome shotgun sequence.
ACCESSION   DBNHLM010000381 DBNHLM010000000
VERSION     DBNHLM010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1137237"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1292
                     /locus_tag="AC16QF_17690"
     CDS             153..1292
                     /locus_tag="AC16QF_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008041547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR6450444.1"
                     /translation="MSEQDHESEKAAEAQPESATAEKAVKRGGQMIGLVIVVTLVWYL
                     LADRYTPYTTQARVQGYVIGVAPKVSGLVTQVWAQNNREVQQGERLFEIDRSEYQIAV
                     GKARSDLENARRQVEAGRAAVEASRANLAAALANRVRAEKDYDRLQRLHSRDPGTIST
                     RRLEMSRASLDQAVAAVSAAESNVQAAIEQMGGEDEASNAILQSAQSAVARAELDLSN
                     TIVTASSRGVITDLRADVGQFAGAGSAVMTLIAVHDLWISAEFTENNLGHMRSGTEVE
                     ILFDVVPGRVFSGRIRSLGLGVSAGQSHPPGTLPTIQNNRDWLRQSQRFAVIVDFDPN
                     QHGELRSQLRIGGQASVMGLTEGHGVLNLLGQLYMRIMSWLSYAY"
     gene            1310..2347
                     /locus_tag="AC16QF_17695"
     CDS             1310..2347
                     /locus_tag="AC16QF_17695"
                     /inference="COORDINATES: protein motif:HMM:NF022616.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2955 domain-containing protein"
                     /protein_id="HIR6450445.1"
                     /translation="MPVQARRVFRLSGVMAVSLALSYAMQMDLPFIAPIFALLLTAAP
                     GPPKGLKALVGLSLVLAVTLGVGLLLVPLLLNYPVTAILIAGAGIYFSNYVSVNLGKG
                     AVGALLTAGVTLISAAGTLSFPLAVTVIEALVLGVGIAVISQWIVYPWFPENESFVPP
                     APPAATPSGSNWIALRATLVVLPAYLLALTNPAMYLPIIMKSVSLGQQSSVVDARDAG
                     RVLLGSTFLGGCFAIVFWFALGVATNLWTFTLLMLLVGVYFSGKLYQVIPTKFPAAFW
                     QNVVVTMLILLGSAVQDSDSGKDVYQAFAVRMSLFVAVTCYAWFAIEVLELLRSRRLG
                     RPEDALGVGGG"
     gene            2350..2553
                     /locus_tag="AC16QF_17700"
     CDS             2350..2553
                     /locus_tag="AC16QF_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450446.1"
                     /translation="MNLLYGLLVMLVLVAGNHAQVGIWGLLFMLLGPLQAIDGILMIG
                     VSTAVLMAAVGNVIRKTAGVDED"
     gene            2550..3188
                     /locus_tag="AC16QF_17705"
     CDS             2550..3188
                     /locus_tag="AC16QF_17705"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR6450447.1"
                     /translation="MTQASPVKIWLVTDPLCSWCWGMSQAVEAAVVRLEGEAEFDFLL
                     GGINVHGTQPIGEYGRRHLMRIWREVQETTGQTFGFRLPDVLVYNSTAACMAVEAVRR
                     LTGKAPFGYLHRLQQLFFVEGRDINDPDLLAGIARDFGCDRNAFAALLDDPAVADHVR
                     DEFAGARLYGTNALPSVLWEGSGGRELLAGGYADADMLVELIRAKRNSQPPA"
     gene            complement(3160..4242)
                     /gene="metX"
                     /locus_tag="AC16QF_17710"
     CDS             complement(3160..4242)
                     /gene="metX"
                     /locus_tag="AC16QF_17710"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015371272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase MetX"
                     /protein_id="HIR6450448.1"
                     /translation="MSATQKFTFQGTFAMRRGSISNPTFAFETLGRLNAQRDNAVLIF
                     TGLSPSAHVASSTADPSSGWWEDMVGPDKPIDTDRYFVVCVNSLGSCFGSTGPASINP
                     ETGKHYRLDFPVLTLEDVANGGAAVLDHLDVQAVNTVLGASMGGMTALAFALLHPDRA
                     RRLLSISSAARSLPFSIAVRSLQREIIRSDPGWKNGNYAFDEGPVTGMRLARKLGMIS
                     YRSADEWAERFGRERSVDGQDADSPFGIDFEVESYLEAHAKKFVGTFDANCYLYLSRA
                     MDLFDATDHGGSLDSALKRVRLEQALVIGVETDFLFPVEQQEELALGLQRYVSDLSFR
                     RLPSIHGHDSFLVDMDRFRPAVASFF"
     gene            complement(4319..5449)
                     /locus_tag="AC16QF_17715"
     CDS             complement(4319..5449)
                     /locus_tag="AC16QF_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007946297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR6450449.1"
                     /translation="MPGLLPNVDPDGLLEYSVVYTDRALNHMSLAFRQVMRDISESLK
                     SVYGAASAVVVPGSGTFGMEAVARQFATGRKCLVLRNGWFSYRWSQILEMGRMPTETI
                     VLKARQAEPGTQAPFAPAPLDEVVATIAAEKPNVVFAPHVETSCGMILPDDYLREVAN
                     AVHAVGGLFVLDCIASGAIWVDMKEIGVDVLISAPQKGWTGTPCCALVMLGTGALGAM
                     EGATSTSFACDLGKWLEIMRAYENGGHAYHATMPTDGLRILADVMREIEAFGYETARE
                     RQEVLGRRVRGLLEGHGIASVAAEGYQAPGVVVSYTTDPDIQSGRKFADAGLQIAAGV
                     PLQCDEPDDFRTFRLGLFGLDKLADVDRTVASLEDALNGVLP"
     gene            complement(5476..5568)
                     /locus_tag="AC16QF_17720"
     tRNA            complement(5476..5568)
                     /locus_tag="AC16QF_17720"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5532..5534),aa:Sec,seq:tca)
     gene            complement(5581..>6123)
                     /locus_tag="AC16QF_17725"
     CDS             complement(5581..>6123)
                     /locus_tag="AC16QF_17725"
                     /inference="COORDINATES: protein motif:HMM:NF020672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB domain-containing protein"
                     /protein_id="HIR6450450.1"
                     /translation="EEQSGLQGLKANQVGAPVPERFKSELLRELAGAGKLTQRAGHYL
                     PAEHEVALSESEARLLDRISGQLDQIQPPSVGDLAKQIGVPVAELKRQLQPLAGKRRL
                     VRVSETRYYLPAPLSSLVHVVDALSSKGPFTVREFRDAADIGRNVAIEVLEYLDGRGY
                     TKRQGDVRRVVGDPSRVMAT"
BASE COUNT         1056 a         1915 c         2009 g         1143 t
ORIGIN      
        1 gggggtggtt ggacggagat gcccatcggt gagctgctgc ccgagaccgt tcgggagaag
       61 atgcgcactc ggagtaattg gggtgacctg ctcgatgcgc cgctgcccgt gaatcaggag
      121 ggccctgtgc cggccgaagg agggacaacc ccatgagcga gcaagatcac gagtcggaga
      181 aggccgcgga agctcaaccg gaatcggcaa cggccgaaaa agccgtcaag cgtggcggtc
      241 aaatgatcgg tctggtgatc gtcgtcaccc tcgtctggta cctcctggca gaccgctaca
      301 cgccatatac cacccaggca agggtgcagg gctacgtcat cggggtggca ccgaaggtgt
      361 ccggcctcgt gacccaggtg tgggcgcaga acaatcgcga ggtccagcaa ggggaacgac
      421 tgttcgagat cgaccgctct gagtaccaga ttgccgtggg caaggcgcgc tcggacctgg
      481 aaaacgcccg cagacaggta gaggccggac gcgccgcggt agaggcttct cgcgccaacc
      541 tcgccgctgc cctcgccaat cgggtcaggg cggagaagga ctacgatcgt cttcagcgac
      601 ttcactcccg ggatcccggg acgatctcca cccggcggct ggaaatgtcc agagcctcgc
      661 tcgaccaggc ggtagccgcg gtctcggcgg ccgagtcgaa tgtccaagct gccatcgagc
      721 agatgggggg cgaggacgaa gcctccaacg ccattctgca atccgcacag agcgcggtcg
      781 cgcgcgcgga gctcgatctg tccaatacca tcgtcacggc ttcttcccgc ggcgtgatca
      841 cggatcttcg cgcggacgtc ggccagttcg ccggtgcggg cagcgcggtc atgacgctca
      901 tcgcggtgca cgacctttgg atcagcgccg agttcacgga gaacaacctg gggcatatgc
      961 gaagcggcac cgaggtggag atcctcttcg acgtggtgcc gggcagagtc ttttccggca
     1021 ggatacgcag cctcggcctc ggagtgagcg ccggccagtc tcatccgccg gggacgctgc
     1081 ctaccattca gaacaaccgg gactggctga gacagtccca gcgctttgcc gtcatcgtgg
     1141 acttcgatcc gaaccagcac ggcgaacttc gctcgcagct gcgcatcggg ggccaggcgt
     1201 cggtgatggg tctcactgaa gggcacgggg tgctgaacct gctgggtcag ctctacatgc
     1261 gcatcatgag ctggctgtcc tatgcttact gatcgggaag gcctctgaaa tgccggtcca
     1321 ggctcgacga gtcttccggc tgagcggcgt gatggccgtc tctctggcgc tcagctacgc
     1381 catgcagatg gatctgccgt tcatcgcgcc gatttttgcg ctgctgctca cggcggctcc
     1441 gggcccgccc aagggcctga aagccctggt cgggctttcc ctggtgctgg cagttacgct
     1501 gggcgtcggg ctgctgctcg ttcccttgct gctcaactat cccgtcaccg ccatcctgat
     1561 cgcgggagcg ggcatctact tcagcaacta tgtgagcgtc aatctgggca agggtgccgt
     1621 cggggcgctt ttgacggcgg gcgtcaccct gatatcggcg gcgggtacgc tgagttttcc
     1681 gctggcggtg accgtgatcg aagcgctggt gttgggtgtt ggcattgcag tcatcagcca
     1741 gtggatcgtc tacccctggt ttccggaaaa cgagtcgttc gtacctccgg caccgccggc
     1801 tgcgactccg agcgggagca actggattgc cctgcgagca accctcgtcg tgctgcccgc
     1861 ctacctgctc gcgctcacca atcccgccat gtacctgccg atcatcatga aatcggtctc
     1921 tcttggtcag cagagctcgg tcgtcgatgc ccgcgacgcg ggccgggtgc tgctcggatc
     1981 gacgtttctc ggtggctgct ttgccatcgt gttctggttt gccctcggcg tggcgacgaa
     2041 cctgtggacg ttcacactgc tgatgctgct ggttggcgtg tacttctccg gcaagctcta
     2101 tcaggtcatc ccgacgaagt ttccagccgc gttctggcag aacgtggtgg tgacgatgct
     2161 gatcctgctt ggctccgcgg ttcaggacag cgattccgga aaggacgtct accaagcctt
     2221 tgccgtgcgt atgagcctgt tcgtggcggt tacctgctac gcgtggtttg caattgaagt
     2281 gctcgagctg cttcgatctc gacgactcgg tcggcccgag gatgcgttgg gcgtaggagg
     2341 cggctgaaaa tgaacctgct ctacggtctt ctcgtgatgc tcgtgctggt tgcgggcaac
     2401 cacgcccagg tgggaatctg ggggctgctc ttcatgctcc tcggacccct tcaagccatc
     2461 gacggaattc tgatgatcgg ggtctctacg gccgtgctga tggccgccgt tggtaacgtg
     2521 atcaggaaga ccgcgggggt cgacgaggat tgactcaggc atcaccggtc aagatatggc
     2581 tcgtcaccga ccccctgtgc tcgtggtgct ggggcatgtc ccaagcggtc gaagcagcgg
     2641 tcgtgcgcct ggagggcgaa gccgagttcg acttcctgct cggcgggatc aacgtgcacg
     2701 gcacccagcc gatcggcgag tacggtcgtc gtcatctgat gcggatctgg cgcgaggttc
     2761 aggagaccac cggtcagacc tttggttttc gcctgccgga cgtcctcgtt tacaacagca
     2821 cggctgcctg catggccgtc gaggcggtga ggcgcttgac cggcaaggca ccgttcggat
     2881 atctgcaccg cctccagcag ttgttcttcg tggagggccg cgacatcaac gatccagacc
     2941 tgctcgcggg gatcgcgcgc gacttcggtt gcgaccgaaa cgcattcgct gcgctgctgg
     3001 acgacccggc agtggccgac cacgttcgtg acgaatttgc cggcgcgcgc ctgtatggca
     3061 ccaacgcgct gcccagtgtg ctgtgggaag gaagcggtgg ccgggaacta ctggcgggcg
     3121 gttacgcgga tgctgacatg ctggtggagc tcattcgtgc taaaagaaac tcgcaaccgc
     3181 cggcctgaag cggtccatgt cgaccagaaa cgaatcgtgt ccgtggatgc tcggcaggcg
     3241 tcgaaaggac aggtcgctta cgtaacgttg cagccccagc gcgagttcct cctgctgctc
     3301 gacgggaaag aggaagtcgg tctccacccc gatcaccagg gcctgttcca gcctgacccg
     3361 tttcagtgcg ctgtcgagac tgccaccatg atccgtggcg tcgaaaagat ccatcgcccg
     3421 ggaaagatag agatagcagt tggcatcgaa cgtgccgacg aacttcttcg cgtgagcctc
     3481 cagatacgat tcgacctcga agtctatgcc gaaggggctg tcggcatcct gcccgtcgac
     3541 gctgcgctcg cggccaaagc gctctgccca ctcgtcggcc gatcggtagg agatcatgcc
     3601 aagcttgcgg gccagccgca tgcccgtcac cggcccttcg tcgaaagcgt agttgccgtt
     3661 cttccagcca ggatcgctgc gaatgatctc gcgctgaagg gaacggacgg cgatggaaaa
     3721 cggcaggctg cgcgctgctg acgagatgga gaggagccgg cgcgcccgat caggatggag
     3781 cagggcgaac gccagcgccg tcatcccgcc catggaggcc cccaagaccg tgttcaccgc
     3841 ctggacgtcc aggtggtcga gaacggcggc cccgccgttg gcgacgtcct ccagcgtgag
     3901 caccggaaag tcgagccggt agtgcttacc ggtttcgggg ttgatggaag ccgggcccgt
     3961 gctgccgaag cagctgccaa gcgagttcac gcagacgacg aaatagcggt cggtgtcgat
     4021 gggcttgtcc ggtcccacca tgtcctccca ccatccggag gaggggtctg ctgttgacga
     4081 tgccacgtgg gccgatggcg acaggcctgt gaagatgagc accgcgttgt cgcgctgcgc
     4141 gttcagccga ccgagcgttt caaaggcgaa ggtcgggttg ctgatggacc cgcgccgcat
     4201 ggcaaaggtg ccctggaatg tgaacttttg cgtggcgctc atgttgccgt cgccagaccg
     4261 atgagttgtc ggtaacagat ccgcccagcg gagctgcgga gggcggatac taacatcgtc
     4321 aggggagcac gccgttcaac gcgtcttcca ggctggcgac ggttcggtcg acgtcggcga
     4381 gcttgtccag cccaaacaga cccaggcgga acgtccgaaa gtcgtccggc tcgtcgcact
     4441 gaagcggcac tcccgcagcg atctgcagac cggcgtcggc gaatttccgt cccgactgaa
     4501 tgtccgggtc ggtggtgtag ctcacgacca cgccgggggc ctgatacccc tcggccgcca
     4561 cgctggcgat gccgtgacct tcgagcagcc cgcgcacccg gcggcccaag acctcctggc
     4621 gctcgcgggc ggtttcgtaa ccgaaggctt cgatttcgcg catgacgtct gcgagtatgc
     4681 gcaacccgtc ggtgggcatc gtggcgtggt atgcgtgccc cccgttttcg tatgcgcgca
     4741 tgatctccag ccacttgccg aggtcgcagg cgaagctggt gctcgtggcg ccctccatcg
     4801 ccccgagggc gcctgttccc agcatcacca aagcgcagca gggagtcccc gtccagccct
     4861 tttgaggtgc gctgatcaac acgtcgacgc cgatctcctt catgtctacc cagatcgccc
     4921 cggaagcgat gcagtcgagc acgaacagcc cgccgacggc atgtacggcg ttggcaacct
     4981 cgcggagata gtcgtcgggc aggatcatgc cgcacgacgt ctccacatgg ggtgcgaaaa
     5041 cgacgttggg cttctcggcg gcgatggtgg caaccacctc gtcgaggggc gccggggcga
     5101 aaggcgcctg ggtgcccggc tcggcctgcc gggccttcag cacgatggtt tccgtgggca
     5161 tgcggcccat ctctaggatc tggctccagc gatagctgaa ccatccgtta cgcaggacga
     5221 gacattttct gccggtggcg aattgcctgg cgacggcctc catgccgaag gtcccgctgc
     5281 cgggcaccac cacggcgctc gccgcgccgt agaccgactt gagtgattcg gagatgtccc
     5341 gcatgacctg ccggaacgca agcgacatat gattgagtgc ccggtcggtg tacaccaccg
     5401 aatactcgag caggccgtcg gggtcgacgt tgggcagcag tccgggcatg tgttcctcgt
     5461 cagtcagcag gaaaatggcg gaagaggtag gagtcgaacc caccaggcac gtcatcgcac
     5521 ctcaacggct ttgaaggccg cgcgccccac cggggacgat gctcttccga aggcagttta
     5581 tcatgtcgcc atcactcggc tcgggtcacc gaccaccctc cggacgtccc cctggcgctt
     5641 tgtgtagccg cggccgtcca gatactcgag cacctcgatg gccacgttgc gcccgatgtc
     5701 ggcagcgtcc cgaaactccc tgacggtaaa aggccctttg ctgctcaagg cgtcgacgac
     5761 gtggaccagg gacgacaacg gggcgggtag gtagtagcgc gtctcgctca cgcgcaccag
     5821 gcgccgcttg ccggcgagag gctgcagctg gcgcttcagc tcagccaccg gcacgccgat
     5881 ctgcttggcg agatcgccga cgctgggagg ttggatctga tcgagctgac cggaaatgcg
     5941 atcgagcagt cgtgcttctg attcgctcag cgcgacctcg tgttccgcgg gcaggtagtg
     6001 cccggcccgc tgagtgagct ttccagcccc ggccagctcg cgcagcagct cggacttgaa
     6061 ccgctcgggt accggcgcgc cgacctggtt ggcttttagt ccctggagcc cggactgctc
     6121 ctc
//
LOCUS       DBNHLM010000382         3044 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_1376191, whole genome shotgun sequence.
ACCESSION   DBNHLM010000382 DBNHLM010000000
VERSION     DBNHLM010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_1376191"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..89
                     /locus_tag="AC16QF_17730"
     CDS             6..89
                     /locus_tag="AC16QF_17730"
                     /inference="COORDINATES: protein motif:HMM:NF012652.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450451.1"
                     /translation="MVGEIRERETAEIAVQAALTVLICTEN"
     gene            complement(146..916)
                     /gene="istB"
                     /locus_tag="AC16QF_17735"
     CDS             complement(146..916)
                     /gene="istB"
                     /locus_tag="AC16QF_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HIR6450452.1"
                     /translation="MSAEAMVTTMRGLKLFGMAQAVSELAQQQGPAFKKTEPVLEDLI
                     KAEVAEREVRSINYQMKAARFPAYRDLSSFEFAEASVDETLIRALHRCEFIDNAENVV
                     FIGGPGTGKTHLATAIGIQAIMHHHLRVRCFSTIELVNQLEIEKANGKPGKIANRLTH
                     ADLVILDELGYLPFSQAGGALLFHLLSKLYEKTAVMITTNLSFSEWASVFGDPKMTTA
                     LLDRLTHHCHIVETGNDSYRFKHSSTRTKKETKTRKSD"
     gene            complement(906..2429)
                     /gene="istA"
                     /locus_tag="AC16QF_17740"
     CDS             complement(906..2429)
                     /gene="istA"
                     /locus_tag="AC16QF_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HIR6450453.1"
                     /translation="MIDVALVSVIRRWHLREGISIREIARRTKLSRNTIRKYLADGTL
                     EPKYPQRKSPSKLDPYAEVLASWLEREAIRSRKQRRNLRQLYGDLVALGYRGSYDRVC
                     AFARAWRRQQQEAARASRGTYVPLVFAPGEAFQFDWSEDWAVIGGERTKLSVAQFKLC
                     HSRAFVLRAYPSMTHEMLFDAHNHAFSVLGGIPKRGIYDNMKTAVDKVGRGKARIVNA
                     RFKAMTGHFLFEAEFCTPAAGWEKGQVEKNVRDARPRIWHEVPKFGDLRALNDWLERR
                     CCVLWEEIRHPEQPARSVAEVWADERAHLMKMPPLFDGFIEETKRVSPTCLVSFDRNR
                     YSVPACFANRPVSLRAYADKLVIAAEGRTIAEHVRVFSRGRDHPVGTVYDWRHYLTVI
                     QRKPGALRNGAPFAELPAAFKSLQRILLKRPGGDREMVDILALVLHHEEQAVVCAVEL
                     ALESGTVSKQHVLNLLSRLLDTEPPAPIDTPERLTLLEEPRANVKRYDHLRGRRHVG"
     gene            2746..>3044
                     /locus_tag="AC16QF_17745"
     CDS             2746..>3044
                     /locus_tag="AC16QF_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450454.1"
                     /translation="MKLTELIPAADDLLSLEPEELAGCLIEYINALPTGDRGNLNRGN
                     FSDRRLVAGYEEGKQPACQRALMEAWAVLEREGLLAHRPEDHHGWYFVTRRGATMT"
BASE COUNT          556 a          924 c          954 g          610 t
ORIGIN      
        1 tcgtcatggt cggcgaaatc cgcgagcgcg agacggccga gatcgcggtg caggcagcgc
       61 tgacggtgtt gatttgcacc gaaaactgac ccgggttttg catttaaaat tgacccgcct
      121 ggtcggccct gagtatggct cagggctagt ctgattttct agttttcgtc tccttcttgg
      181 tcctggttga gctgtgcttg aagcggtagc tgtcgttgcc ggtctcgacg atatggcaat
      241 ggtgcgtgag ccgatcgagc agcgcggtgg tcatcttcgg atcgccaaac acggaagccc
      301 attccgagaa gctcaggttg gtcgtgatca tcacggcggt tttctcgtag agcttgctca
      361 ggagatggaa cagcagcgcg ccgccggcct gtgagaacgg cagatagccg agctcgtcga
      421 ggatgacgag atcggcgtgg gttaggcggt tggcgatctt gccgggtttg ccgttggcct
      481 tttcgatctc gagctggttg acgagctcga tggtcgagaa gcaacggacc ctcaaatgat
      541 gatgcatgat ggcctggatg ccgatggcgg tggccaggtg cgtcttgccg gtgccgggtc
      601 cgccgatgaa gacgacgttc tcggcgttgt cgatgaactc acagcggtgc agggccctga
      661 tgagcgtctc gtcgacgctg gcctcggcga actcgaagct cgacagatcc cggtaagccg
      721 ggaatctcgc agccttcatc tgatagttga tcgagcggac ctcgcgctcg gcgacttcgg
      781 ccttgatgag gtcctcgagc acgggctcgg tcttcttgaa cgccggtccc tgctgctggg
      841 cgagctccga gacggcctgc gccataccga agagcttcaa gccccgcatc gtcgtgacca
      901 tggcctcagc cgacatggcg gcggcctcgt aagtggtcgt agcgcttcac attggctcgg
      961 ggctcctcca gcagcgtgag tcgctcgggc gtgtcgatcg gcgccggcgg ctcggtgtcg
     1021 aggaggcggc ttaagaggtt gagcacgtgc tgcttggaga ccgtgcccga ctcgagcgcg
     1081 agctccacgg cgcagacgac cgcctgctcc tcgtggtgca gcaccagggc gaggatatcc
     1141 accatctccc gatccccgcc ggggcgcttt aggaggatcc gctgcagcga tttgaaggcc
     1201 gccggcagct cggcaaaggg cgcgccgttc ctgagcgcgc cgggcttgcg ctggatcacg
     1261 gtcagataat ggcgccagtc gtagaccgtg ccgaccggat gatcgcggcc gcggctgaag
     1321 acccggacgt gctcggcaat cgtgcgacct tcggcggcaa tgacgagctt gtctgcatag
     1381 gctcgcaagc tcaccggccg gttggcgaag caggccggga cgctataacg gttacggtcg
     1441 aagctcacca ggcaggtcgg cgaaacccgc ttggtctcct cgatgaaacc gtcgaacagc
     1501 ggtggcatct tcatcagatg agcccgctca tcggcccaga cctcggccac ggatcgagcc
     1561 ggttgctccg gatggcggat ctcctcccag agcacgcagc agcggcgctc gagccagtcg
     1621 ttgagcgccc tcagatcacc gaacttcggt acctcgtgcc agatccgcgg gcgcgcatca
     1681 cggacgttct tctccacctg gcctttctcc cagccagctg ccggcgtgca aaactccgct
     1741 tcgaacaaaa aatgcccggt catggccttg aagcgggcgt tgacgatccg ggccttgcca
     1801 cgcccgacct tatccaccgc cgtcttcatg ttgtcgtaga tgccgcgctt ggggatgccg
     1861 ccgagcacgc taaaggcgtg gttatgggcg tcgaacagca tctcgtgcgt catcgacgga
     1921 taggcccgca gcacaaacgc ccggctgtgg cagagcttga actgggcaac agacagcttg
     1981 gtccgctccc cgccgatgac cgcccagtcc tcgctccagt cgaactgaaa cgcctcgccc
     2041 ggggcgaaca ccagcggtac gtaggtgccc cggcttgcgc gggcagcttc ctgctgctgc
     2101 cggcgccagg cgcgagcaaa agcacagacc cggtcgtagg agccccgata gcccagggcc
     2161 acgaggtccc cgtacaactg ccgcagatta cgccgctgtt tgcggctccg tatcgcctcg
     2221 cgctccagcc acgaggccag cacctccgca tacggatcca gcttgctcgg gctcttgcgt
     2281 tgcgggtact tcggctccag cgtcccgtcc gccaagtact tgcgaatggt gttcctggag
     2341 agctttgtgc gcctggctat ctcgcgaatc gatatccctt cccgaagatg ccagcgccga
     2401 atcacactga ctaacgccac gtcgatcact ccttacatct ccctgctctc caaagcaggg
     2461 caagggttta cacgtgggtc agaattagat gcaaatacct agcctgggtg ggtcagtttt
     2521 ggatgcaaat caacattcgc taatctctca gccaacctcg gtctgagcat ttccgggtaa
     2581 ttcgcccaga ttcggcgctc gacgagtgct cgactgacta ggtagattcc tctctgcccc
     2641 tgcactcgcc acgccatata cgccgggcca gaaaaccggc ggcgggtgtt ggggttgctt
     2701 cgcttcggcg gggctttttt gtggcagact ctttcgctta gaacaatgaa actaacagag
     2761 ctaattccag cggcggatga tcttctgagc ctagagcccg aggagttggc tggctgtctg
     2821 atcgaataca tcaacgcgct tccgacgggc gaccgtggga atcttaatcg gggcaacttt
     2881 agtgacaggc ggctagtggc tggctacgaa gaagggaaac agcctgcttg ccagcgcgct
     2941 ttgatggaag catgggcagt cctagagcgg gaaggattgc ttgctcacag accagaagat
     3001 catcacggct ggtacttcgt tactcgaaga ggcgcgacga tgac
//
LOCUS       DBNHLM010000383        16583 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_478038, whole genome shotgun sequence.
ACCESSION   DBNHLM010000383 DBNHLM010000000
VERSION     DBNHLM010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 16583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16583
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_478038"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..620
                     /locus_tag="AC16QF_17750"
     CDS             138..620
                     /locus_tag="AC16QF_17750"
                     /inference="COORDINATES: protein motif:HMM:NF042724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6640 family protein"
                     /protein_id="HIR6450455.1"
                     /translation="MPRSFRAELMPVDNPFDQNLIPRILMSLMALAIAIGPMFADFNK
                     THATNPLWPPHARFHVVWQVLTQAGISVVALVLLWRASPDYVLHLYLAAALIYVWLLT
                     FFMTLASIKVYQGALRDVNGIEPFRFNIMGKVREVDTNLFGVVLFTVLNTVAALLIRG
                     "
     gene            628..1680
                     /locus_tag="AC16QF_17755"
     CDS             628..1680
                     /locus_tag="AC16QF_17755"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR6450456.1"
                     /translation="MRIIVIVACVAIVLAGALFGAGLYFSAPVFDERVSEPPDGAFDI
                     LPVSEALTFARTKDGRLLLVRSADADAIEAVDLGAALDTVATDPLAILRTHGYQRVAA
                     RLTGVAQAVPLEQLGMPFDPGYPHIAAGTNFRAHAEEVGLDEGPFLFPKLTRATAWDA
                     RVPARTRLDYEVELCAVALGVYSADGPAPLGFVLCNDFTDRWALLTEIDLDTPMGQSG
                     FPDAKGGAGMLPIGPFLLVPSRDADFHRRLEIGLAVNDRLRQRDSAGLMIWSPHEIAL
                     TALENCEAPFVSTAGSHRLTPCDAIDEATLLLTGTPAGVAFHFLNIWRPGSYLRSGDR
                     VLAYGSHLGVLRNTIE"
     gene            1820..3019
                     /locus_tag="AC16QF_17760"
     CDS             1820..3019
                     /locus_tag="AC16QF_17760"
                     /inference="COORDINATES: protein motif:HMM:NF014954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6450457.1"
                     /translation="MNVQDDVPYTVEDLTAEWLSEGLKESGVLEGAAVTGFTMDVLGD
                     QVGFNGEVVIIAPTYSPPSASAPASLVLKIPTASKNRIMGQTMGLYEKEIRFYRDLQP
                     GLNIRTPRHYFSALDVADDPDVILERLRGMNRLPIWMIRGIVALANRFVSGNPRRYAL
                     LIEDLSGYRMGDQAAGCSDEDARHIVAAMARLHAQFWASETLDEKSWITPYAVTSRIM
                     QMRFLQGVDQYLCDEGGSLTPGRRDLIGWLRKNGIALTETFGDEPATLLHGDVRLDNI
                     CFDDATGEVVLFDWQTILAGPGASDLAYFISATLPGDADESQVDELIDVYHGELSQHG
                     VDVSRDRLRWQYEVGMLTMLHRVLPTTYPDQMELDAGRGLPLLQAWISRIYNRLEQID
                     HERILKI"
     gene            complement(3110..3469)
                     /locus_tag="AC16QF_17765"
     CDS             complement(3110..3469)
                     /locus_tag="AC16QF_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450458.1"
                     /translation="MAWHSAGCRVHRGAGATLYLQNLLNFDWEQTRSPAGAIIRLSTD
                     GSLRQSVPDAHVVGKFNPPVMTTADLALKFDPEYRKVAERFLADPDACQRQLKYTATP
                     VDLIFGSTAELRAIAAV"
     gene            complement(3545..5065)
                     /locus_tag="AC16QF_17770"
     CDS             complement(3545..5065)
                     /locus_tag="AC16QF_17770"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0032787 - monocarboxylic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6450459.1"
                     /translation="MSVKAYDWVAYHARQTPEKLALVDLHSNRQFSYRDMQERTARLA
                     GAMRDGFGVRPGDRVAALLNNSSDCMEMNFACMKLGAIYVPLNWRLTVSELEYIVGDA
                     EPSLLIHEDVFSDEAEALKERCSVRALLETTASGTGNAYEAAIDAAAPLTGSAEITHD
                     DTWVIMYTSGTTGHPKGCMITYGMTFWNVVNLLNPHRLSEDMVNLITLPLFHTGGLNV
                     YGNPAVHMGGTNIVMRSFDPGETLRILQDAEYGITHMIGVPANYLFMSQHPAFAEADL
                     SRLRAMAVGGAPTSLELLKIYADKGNALQQAFGMTETSPLVTALTPANALDKPGSAGL
                     AVLHTEIRLVDEEAREITAPNVVGELWVKGPNVTPGYWRRPDANESSFTDGWLHTGDA
                     AYQDEDGFIYIVDRWKDMYISGGENVYPAEVENVIYQLPAVAEVAVIGVPHERWGETG
                     QAVIVLKEGESLSEEAVLKHCKDKLARFKQPSSVRFIDEIPHNATGKQLKRELREQ"
     gene            complement(5220..6806)
                     /locus_tag="AC16QF_17775"
     CDS             complement(5220..6806)
                     /locus_tag="AC16QF_17775"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR6450460.1"
                     /translation="MKKQLNPKALFASYLLLVVGCAPAEPGLGEQDAFQQDYWIENYV
                     GDLSFPWSLAWLPDGSLLITERLGKIKRVRNGEIVGEIAGVPEVMTASPYDGLLDIKL
                     DPDFQTDPFVYLTYTRGTATARTGVVYRARLDGESLVDGRELFSTSPPAPAGGPNITR
                     IQFLPDKSMVIAVGSSGNPGNGMVQRVDGDIGKIIRINRDGTIPADNALAAIAPDARP
                     ELWATGLRSLGGFALDDDNRLWGLDIGPQGGDELNLLEPGGNYGWPLVTWGFDYSGKA
                     LSDRQTAAGFVDPVVVWSPSIAPSGLVYYRGDAFPSWQGDLFVGSLADQSIRRLRIRG
                     TTLVHQERLLPDLNERIRSLATGPDGFLYALTDSSNGKILRVRPGRPAPHELARVAQP
                     FEMTEGADIFEKLKEHGVMQSEETSLADQAAYDPARAETLFVQTCGSCHVFEDAGSGN
                     IGPKLDGVVGRRSGTLPGFSYSEALANSETSVVWGYFTLTAFLTNPQAYYPGNRMAAA
                     PLRYEDALQISMYLNHGETF"
     gene            complement(6848..7282)
                     /locus_tag="AC16QF_17780"
     CDS             complement(6848..7282)
                     /locus_tag="AC16QF_17780"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6450461.1"
                     /translation="MKQTQPIECGIPVVDLDRMLEFYCSVLGCRESRRADIPAQLSQR
                     LSVAPDGYVNVWLETPGGEIIKLMRPPEAPEPNNPPEHLATRGGIAYLTFYCSDLHET
                     LAAAEAQGAELRSDRALIDPERPLRLCFFSDPEGNIIELVET"
     gene            7669..7989
                     /locus_tag="AC16QF_17785"
     CDS             7669..7989
                     /locus_tag="AC16QF_17785"
                     /inference="COORDINATES: protein motif:HMM:NF019491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HIR6450462.1"
                     /translation="MSSTNRMIRHIVLVKFREDAPDELRQELVRLSQWGRDADYVSGY
                     VCGWGMQPNLYPGAGEEWDWGMSLDMAEEDAKRYAEDPTHTAIPQEVIDCAEKFAILD
                     FLME"
     gene            8057..8689
                     /locus_tag="AC16QF_17790"
     CDS             8057..8689
                     /locus_tag="AC16QF_17790"
                     /inference="COORDINATES: protein motif:HMM:NF016087.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE"
                     /protein_id="HIR6450463.1"
                     /translation="MNDVRKLGALLCLLAGCGACGPTGDAPGITAAPDPADDATQTPV
                     DGHSSRDSLDWSGVYSGVTPCADCPGIRTTLTLHGDGSFERTRFYLERSAAPQRQAGQ
                     FLWNASGSAITLQEPEGHGQHYKVAENALIHLDRAGKPITGELAGRYVLSKHRRDPAI
                     EGRRWTLVALQGRPLEADAPGSTPSLLLDPEQSLAAESCEPGLHTANHSL"
     gene            complement(8677..9405)
                     /locus_tag="AC16QF_17795"
     CDS             complement(8677..9405)
                     /locus_tag="AC16QF_17795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6450464.1"
                     /translation="MRNLAEAFRRLARTTRFRLSAEGRKPHAYCCPVCGYRGPFADLV
                     QPQGVRLHARCPACRCLERHRLQALVLDGLDQRLFRNARILHLAPEPFLEQRFRTLSR
                     RYISADRFAGGVDCLANLEQLPFADESLDLVYASHVLEHVQDDRRAIAEIHRVLRPGG
                     LAILPVPIQAQQTVEYGEARADEHHHVRAPGLDYYDRYRSVFERVDEVASGDFDPRYQ
                     LFALEPHEGRVRARRDLVPLCYKE"
     gene            complement(9402..10535)
                     /locus_tag="AC16QF_17800"
     CDS             complement(9402..10535)
                     /locus_tag="AC16QF_17800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6450465.1"
                     /translation="MVVAQRLGLPSQTWVARQLQTMNAFTPSVLTWETVAGTPPLGAP
                     VVELPFDPEPYAGAGRWMFRLRHAGDRNFFGALGAEARALSQELGALDPDVVLCHFGH
                     TALRLLPVARSLGIPLVAHFHGMDLAAMLDRWFYRSSLLRHFRHFDASIVVGSFQLRI
                     LEGLGLPTDQVHVIPCGVPTQMFVPGQRTEPGPIRFVSVGRLVEEKGMEECLLAFAKL
                     GQALGDAELTIVGEGPDRERLRALTDSLGITSRVTFAGALTPAAVAELLSGSDIYVQH
                     SHREGFGVALAEAASAGLPVVSTDVGGIPDQVVANETGFLIDVGDVAGMARAMARLGN
                     DPALRAQLGTAGRRRMVEKFDTRSQTGKLEAVLLSVLKDADRK"
     gene            complement(10584..11393)
                     /locus_tag="AC16QF_17805"
     CDS             complement(10584..11393)
                     /locus_tag="AC16QF_17805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIR6450466.1"
                     /translation="MDSESSISVRVEGVALNFRGVGADDHVLAVMRESGAIYEQSVLL
                     KILHQLNGRKGAAVDAGAFIGTHSVFFSRICGLAPVLAFEADPETFTVLSQNLKDNDA
                     RSVVAQNVALGNRIGRAELRQGAAGNRGTVSVDPRDDGTLAITTIDVAVAEMAGWEPV
                     VLIKIDVEGAEIDVLQGAEETITRDMPLLCVEAHGPAQFAELARFLHSCGYGIVDCLG
                     GSPTYIADVTRTGDLKAVLSRRIWQLRSRMRGTWTRAAMRRLAIIVGPWQN"
     gene            complement(11386..12822)
                     /locus_tag="AC16QF_17810"
     CDS             complement(11386..12822)
                     /locus_tag="AC16QF_17810"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR6450467.1"
                     /translation="MITLAAQGIKLAVQISAIAILARLLAPADFGLIAMITAVTGFLF
                     LLKDGGLSMATMQSIVLSPQQVTNLFWINAGLGLTLMLITMALAPLVAWFYGEAKLVG
                     ATCAISLVFFMGGLHTQQQAMLNRQMRYRLLAGIEISAMVAGAAAGVLWALAARNYWA
                     LILMPLAQAAVSTLLYYLCCDWRPGLPKRLFETRAQLGFAGRLTVSGIAGYAARNADN
                     LLIGWYWGAAALGYYNQAYRLLLMPVRQINDPLTRIAVPVLSRLAREPDRFRRYFLGI
                     LGAILWLTMPAVALLVAFADTIILLVLGPGWGETVVLFRALALAALVQPVLNALGWLY
                     IATGRAGGFMKVSMVNAVVLIVAFAAALPFGTGAMAWTFSGVVVGLMLPLTAIACRDT
                     PVAMKDVINTAASPVTLSVLMLCVALLSEDLLARWLERGLLAEAAAALVSILCVLATA
                     CVWKRSRQQLSTLLPALRQSVLDGGGNG"
     gene            12981..14306
                     /locus_tag="AC16QF_17815"
     CDS             12981..14306
                     /locus_tag="AC16QF_17815"
                     /inference="COORDINATES: protein motif:HMM:NF024968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6450468.1"
                     /translation="MSRQQQTHRNRLKVAFSPFWGSDGHAGMPRIERFVRWLQHAGAS
                     IVVVAAGCEDDLSREEAIVVYRSRDPLGLYRTDPDRPAPAPRRPNRFRRMLSYLLFVP
                     DPSRVWASRLAADRKLLEVLQGTTHVLASSPPESVHPGAARLARRLGAQYIMDMRDGW
                     LDEPLRPLLQRSALRRWLERRLEARCIAAAAAILVTSDSWRSLLSARYRLAEPRIRVL
                     TNAYPPTRHYPPAAPVSAGLLVHAGRFEGSDPRRRPAGLLEPLLSAVCRNQSLQGEIL
                     LPGAHTPGDLLALESFATEFAAIGWKLRISTPISRPQLLSELNIAGGLLLLSASRAQI
                     PGKLFEYLPTGRPILALVPGDGATWRLCEALEQVFLVDAGNPGAFAGRIDEFLAAAHD
                     DKRFPVPPEYSEDALAEVFLSSLVTPECAITADDSAGAGAKGLNGKADL"
     gene            complement(14303..14980)
                     /locus_tag="AC16QF_17820"
     CDS             complement(14303..14980)
                     /locus_tag="AC16QF_17820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HIR6450469.1"
                     /translation="MEYDEVVLGRRSIRGFSKEPVPKALIREVVEIAMRAPSSLNTQP
                     WHFHVVSGEPLDRIREGNTERNLAGVPDSREFRGHGGYKGEHRERQKQIAGQLFEAMG
                     IDWGDKPRRQDWVLRGFRQFDAPVSVVVTYDRSIHGGDIAPFDCGAVTNALVNAAWSR
                     GLGCVINSQGIMQSPVVREHARIPDDEVIMICVAMGFPDDSFPANAVVSERKPVDEAV
                     SFVGFQD"
     gene            complement(15086..16348)
                     /locus_tag="AC16QF_17825"
     CDS             complement(15086..16348)
                     /locus_tag="AC16QF_17825"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6450470.1"
                     /translation="MTRDSGGIHAIRVIDVDAHLHEPLDWIEQTDPALAGKLGPPARF
                     MDVANSVFGIANPALAELSEGQPPSGGYETILPGFVHHLEMTDQRQPERQCGSDDDPF
                     CGAEARLAFCDERGIDVQFLNPTFLVGAFVQAARARRFELMGEIRQCWNRWAADQVHG
                     HADRLIPVTQIDLSNVPRSIEEMTRMRERGSRAFAIPESPVGKPRFARNGQPALAKSL
                     THPDFEPLWSAAEDLGMAAIAHVGFSRERINPGWANNGADDLMTYSFLNMVVNPQLAP
                     QLLLAALAFDGVFERHPKLTVIVEEVGIAWLPPLLDALDQGVGRAGHRALEDGAYRPD
                     FAGSAYNLPLSPSEYLRRQVRVTPLVASQPLHPTIDLVPEMLCFSSDYPHVEGSGEAM
                     AVCERQLGTLSDEAHRAFYAGVGEMIGL"
     gene            complement(16345..>16583)
                     /locus_tag="AC16QF_17830"
     CDS             complement(16345..>16583)
                     /locus_tag="AC16QF_17830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6450471.1"
                     /translation="SRRDRSLVVVAILTALGQEAELAFHIPWGLNHGLTRAEVEEIMT
                     HLCLYTGFPRAVEGMRAAKHAFAELDGQATAQPQ"
BASE COUNT         2893 a         5317 c         5272 g         3101 t
ORIGIN      
        1 ctctgagggc ttgcaggagg ctcagttaca gcgcacctgt gagcacgtcc tgcgcgttct
       61 ggggctgacg cccgaggagg ccaggcgcga agtggagggc gtcgccgggc atgtgctgct
      121 cggagacgaa ctgagtcttg ccgagatctt tccgcgctga gttgatgccc gtggacaacc
      181 cgttcgacca gaatctgatt ccccgcatcc tcatgagcct gatggccttg gccatagcga
      241 tcggtcccat gtttgccgat ttcaacaaaa cccacgccac gaacccgctg tggccgccac
      301 atgcgcgctt tcacgtggtc tggcaggtgc tgacccaggc tggtatcagc gtggtcgccc
      361 tggtgctgct gtggcgagcg tcccctgact atgtgttgca cctctacctc gcggccgcgc
      421 tcatctatgt ctggctcctc accttcttca tgaccctcgc ctcgattaag gtttaccagg
      481 gcgcgcttcg ggatgtgaac ggcatcgaac ccttccggtt caacataatg ggcaaggtgc
      541 gcgaggtgga cacgaatctc ttcggtgtgg ttctttttac cgtgctgaat acggttgccg
      601 cgctgctgat ccgggggtga acgagtcatg aggatcatcg tcatcgtcgc ctgcgtggcg
      661 attgtgctcg ctggggcgct ctttggtgcc ggcctctatt tttctgcacc ggttttcgac
      721 gagcgcgtct cagaaccccc cgatggtgcc ttcgacattc tgcccgtcag cgaggcgctg
      781 acctttgccc gcacgaagga tggccggctg ctgcttgtgc gcagcgccga cgcggatgcc
      841 atagaggccg tcgacctcgg cgccgcgctc gacacggtgg caacagaccc gttggccatt
      901 ctgcgcacac acggctacca gcgggtggcg gcaaggctga caggcgtggc ccaagcggtt
      961 ccgctggagc agctcggcat gccgttcgac cccggctacc cgcacatcgc tgcggggacc
     1021 aatttccgcg cccatgccga ggaagtgggc ctggacgagg ggcccttcct gtttcccaag
     1081 ctgacccggg ctacagcgtg ggacgcacgg gtaccggcac gtacgcgcct ggattacgag
     1141 gtcgagctct gcgccgttgc cctcggcgtc tactccgccg acgggcctgc cccgctgggt
     1201 ttcgtgctgt gcaacgactt taccgatcgc tgggcgctcc tgacggaaat cgacctggac
     1261 acgccgatgg gccaatccgg atttcccgac gccaagggcg gggcgggcat gctgcccatc
     1321 gggccattcc tgctggtgcc cagtcgcgat gcggactttc accggcgcct ggaaatcggc
     1381 ctcgcggtca acgatcgtct gcggcagcgc gactccgcgg gcctgatgat ctggtcgccc
     1441 cacgagatcg ccttgaccgc gctcgagaac tgcgaagcgc ccttcgtcag caccgccggc
     1501 agccaccggc tcacgccgtg cgatgccatt gacgaggcga cgttgcttct taccgggacg
     1561 ccggccggtg tcgcgtttca ttttctgaac atatggcggc cgggctctta cctgcgatcg
     1621 ggcgatcggg tgcttgctta cggttcgcac ctgggcgtgc ttcgcaacac catcgagtga
     1681 gcgctctcaa cgcgcggacg tcgtgttgac cctgcgtgac ttttggttgc tagaatgatg
     1741 gatgtgacat ttcggagccg gatgcgcgag cccctgcagt ccgtactccg aaaagcgatc
     1801 tccgctccgg atccgaacga tgaacgtaca agacgacgtg ccctacaccg tcgaggacct
     1861 gaccgccgag tggctgtctg aggggctcaa ggaatccggc gtgctcgagg gcgccgccgt
     1921 caccgggttc accatggacg tgctcggtga ccaggtgggc ttcaacggcg aggtcgtcat
     1981 catcgcgccg acttactcgc cgccctcggc aagcgctccc gccagcctgg tcctcaagat
     2041 ccctacggcg tccaagaacc gcatcatggg gcagaccatg gggctgtacg aaaaggagat
     2101 ccgcttctac cgggacctgc agccgggtct gaacatccgg acaccccgtc actacttcag
     2161 cgcgctggat gtggctgacg atcccgacgt gatactcgag cggttgcggg gcatgaaccg
     2221 gctgccgatc tggatgatcc gcggcatcgt cgcgctcgcc aaccggttcg tgagcggcaa
     2281 cccgcgtcga tatgcgctgc tcatcgaaga cctgagtggc tatcgtatgg gagatcaggc
     2341 tgctggttgc tctgacgagg atgctcgcca catcgttgca gccatggctc gcctccacgc
     2401 ccagttctgg gccagcgaaa cgctcgatga aaagagctgg atcacgccat acgccgtcac
     2461 cagcaggatc atgcagatgc gttttctgca gggtgttgat cagtacctct gcgatgaggg
     2521 cgggagtctg acgccgggcc gccgggatct catcggctgg cttcgaaaga acggcatcgc
     2581 gctcacggag acgttcggtg atgaaccagc caccctcctg cacggagacg ttcgcctgga
     2641 caacatctgt ttcgatgatg ccaccggcga ggtcgtgctg tttgactggc agacgatcct
     2701 ggccggtcca ggcgcttcag accttgccta cttcatcagc gcgacgctcc ccggggacgc
     2761 cgatgaatct caggtcgacg agctcatcga cgtctaccac ggtgagcttt cgcaacacgg
     2821 ggtagacgtc tcacgggatc ggctgcgctg gcagtacgag gtgggcatgc tgacgatgct
     2881 gcatcgggtg ctccccacca cctatccgga ccagatggaa ctcgatgccg ggcgcggctt
     2941 gccgttgctg caggcctgga tctcgcggat ctacaaccgg ctcgagcaga ttgaccacga
     3001 gcgtattctt aagatctgaa gcctacaggt cgaagcggtc cagccgcatg ccattgaccc
     3061 aggcggcgac gaagtcgctc aggaaaagtt ccttcccgtc gtcgtaggcc tagaccgcgg
     3121 caattgccct cagctcggcg gtggagccga agatcagatc cactggcgtg gcggtgtact
     3181 tgagttgccg ctggcacgca tccggatcag ccaggaaccg ctccgcgact ttcctgtact
     3241 ccggatcgaa cttcagcgcg agatcggcgg tggtcattac gggcgggttg aacttgccca
     3301 cgacgtgggc gtccggcacg gactggcgta gcgatccgtc cgtggagagc cggatgatgg
     3361 cgcccgcagg gctgcgggtc tgctcccagt cgaagttcag caggttctgc aggtacagcg
     3421 tcgcccctgc tccccgatgg acgcggcacc cggcgctgtg ccatgccatg cggatgaaca
     3481 gccgacctta gttacccaac tcggccggcc accagaactg agttgaacgc ggctggccga
     3541 gggctcattg ctctctgagc tcgcgtttga gctgcttgcc ggtggcgttg tgcgggatct
     3601 cgtcgatgaa gcgcaccgag ctcggctgct tgaaccgtgc cagcttgtcc ttgcagtgct
     3661 tcagcacggc ttcttccgac aggctttctc cctctttcag cacgatcacg gcctggccag
     3721 tctcgcccca gcgctcgtgg ggcaccccga tcacggccac ctcggccacc gccggcagct
     3781 ggtagatgac gttctccacc tccgccggat agacgttttc accccctgag atgtacatgt
     3841 ccttccagcg gtccacgatg tagatgaacc catcctcgtc ctgataggct gcatcccccg
     3901 tgtgcagcca gccgtctgtg aacgagcttt cgttcgcgtc cggtcggcgc cagtaaccgg
     3961 gcgtcacgtt cgggcctttc acccacagct ccccgaccac gttcggcgcg gtgatctccc
     4021 gggcctcctc gtcgaccaga cggatctcgg tatgcagcac cgcaagccct gccgagcctg
     4081 gcttgtccag cgcgttggcg ggcgtcagcg cggtcaccag ggggctggtt tcggtcatgc
     4141 cgaacgcctg ctgcaacgcg ttgcccttgt cggcgtagat cttcaggagc tccagtgacg
     4201 tgggcgcccc gcctaccgcc atggcacgaa gtcgggagag gtcggcttcg gcaaatgccg
     4261 gatgctggct catgaacaga tagttggcag gcacgccgat catgtgggta atgccgtatt
     4321 cggcatcctg gaggatgcgc agggtctcgc ccgggtcgaa cgagcgcatc acgatgttgg
     4381 tgcctcccat gtgcacggcc ggatttccgt agacgttcaa cccccccgtg tgaaacagcg
     4441 gcagcgttat caggttcacc atgtcctctg acagccgatg ggggttcaac aggttcacca
     4501 cgttccagaa cgtcatgccg taagtgatca tgcagccctt ggggtggccg gtggttccgg
     4561 aggtgtacat gatcacccag gtgtcgtcgt gggtgatctc cgcgctcccg gtcagcggtg
     4621 cggccgcgtc gatcgccgcc tcgtaagcgt tgccggttcc gcttgcggtg gtttcgagca
     4681 aggcgcgaac gctgcatcgc tccttgagcg cttcggcctc gtcgctgaaa acatcctcgt
     4741 ggatcaacag gctcggctcg gcgtcgccaa cgatgtactc cagctcggac accgtcagcc
     4801 gccagttgag aggcacgtag atggctccga gcttcatgca ggcgaagttc atctccatgc
     4861 agtccgaaga gttgttcagc agcgccgcca cgcggtcacc gggccgaacg ccgaaaccgt
     4921 cgcgcatcgc ccccgcgagt ctcgccgttc gttcctgcat gtcgcgataa gagaattgcc
     4981 gattcgaatg caggtcgacc agcgccagct tctccggcgt ctgccgggcg tgataggcga
     5041 cccagtcata ggctttcacg gacatctgcg ggctcccctc cctctcgatg atggggagta
     5101 tagacgatgg cttgcagcct gcgccccgca ggccgcagga gccgcgaacg ggttcgctgc
     5161 ccgcggcaac gtcacccaaa cggcccgggg acctggccag tcggacgcgt cgatgtcagc
     5221 tagaaggttt ccccatggtt caggtacatg ctgatctgca gcgcgtcttc gtagcgcagc
     5281 ggcgccgccg ccatcctgtt gccaggatag taggcctgcg ggttggtcag gaaggcagta
     5341 agggtgaagt atccccagac gacgctcgtt tcgctgttgg ccaaggcctc ggaatacgaa
     5401 aatccgggca gcgtgccgga tcggcggccc acgacgccat ccagcttcgg gccgatgttg
     5461 ccgctgccag cgtcctcgaa cacatggcag cttccgcaag tctgcacgaa cagggtttct
     5521 gcgcgcgccg ggtcgtaagc ggcctgatcc gccaggctgg tctcctcgct ctgcatgacg
     5581 ccgtgttcct ttagcttttc gaatatgtct gcgccttcgg tcatctcgaa aggctgagct
     5641 acccgggcca gctcatgtgg cgccggtcgt cccggacgga ctcggagaat cttgccgttg
     5701 gaagaatcgg tcaacgcgta gagaaagccg tcgggtcctg tggccaggga gcggatgcgt
     5761 tcgttgaggt cgggcagcag tcgttcctgg tgcacgagcg tggtaccgcg aattctcagg
     5821 cgcctgatgg actgatcggc gagagagccg acgaagagat cgccctgcca gctgggaaag
     5881 gcgtcgccgc gatagtagac gagcccggaa ggcgcgatgg acgggctcca caccaccacc
     5941 gggtcgacga acccggcagc cgtctgccgg tctgagagcg cttttccgga atagtcgaat
     6001 ccccaggtca ccagcggcca gccgtagttt ccgccgggtt cgaggaggtt cagctcatcg
     6061 ccaccctggg gaccgatgtc cagaccccac agccggttgt cgtcatccag ggcaaagccc
     6121 cccagggatc tcagaccggt ggcccacagt tccggcctcg catcgggggc gatggcggcg
     6181 agcgcgttgt ccgcggggat cgtcccatcc cgattgatgc gaatgatctt gccgatgtct
     6241 ccatcgaccc gctgcaccat gccgttgccg gggttgccgc tggacccgac ggcgatcacc
     6301 atggatttgt ccggaagaaa ttgaattcgc gtgatgttcg ggccgcctgc tggtgcaggc
     6361 ggcgaggtgc tgaacagctc ccgtccgtcg accaggctct cgccatccag ccgcgcccgg
     6421 taaacgacgc cggtccgcgc cgttgccgtg ccccgggtgt aggtgaggta aacgaacggg
     6481 tcggtctgga aatccggatc gagcttgatg tcgagcaggc cgtcgtaggg actggccgtc
     6541 atcacctcgg gcaccccggc aatctcgccg acgatctcgc cgttgcgaac ccgcttgatt
     6601 ttgcccagcc gctcggtgat caggaggctg ccgtccggga gccaggccag ggaccagggg
     6661 aagctcagat cgccgacgta gttctcgatc cagtagtcct gttggaaggc gtcctgttca
     6721 ccgaggccag gctcggccgg cgcgcaaccg acgaccagca gcagatacga ggcgaaaagg
     6781 gccttggggt tcagttgctt tttcattttg ttcactttcc agttgccctt tgcgcgcgtc
     6841 cgcaggttca ggtttccacc agctcgatga tgttaccttc aggatcggag aagaagcata
     6901 agcgtagcgg tctttccggg tcgatgagcg cgcggtccga gcgcagctcg gcgccctgag
     6961 cctcggcggc cgccagggtc tcgtgaaggt cgctgcagta gaaggtcaga taggcgatcc
     7021 cgcccctggt tgccagatgc tcggggggat tgttcggctc cggcgcttct ggtggcctca
     7081 tgagcttgat gatttccccg ccgggcgtct ccagccagac gttgacgtaa ccatccgggg
     7141 ccaccgagag ccgttggctc agctgggcgg gaatgtccgc gcgccgggac tctctgcagc
     7201 ccagcacgct gcaatagaac tcgagcatcc ggtccaggtc gaccacggga atgccgcatt
     7261 cgatgggttg ggtttgcttc atcggttttc ctcgttggcg gcggccgccg gttctggggc
     7321 ctcggcggca gcgcgcgccg ttgtctggcc tgccccgccc ggcgaatcgg agatcggtgt
     7381 gagagcgtca tggattctgc ctgcgcgctt ctcggctgga aagcccttcc cggggttcct
     7441 caacacgtct ggcggcgggc gcggatcccg acgaagcacg gcggcgtgag tatatttttc
     7501 gatcggtggc ggttcgtctg aagcgttgtc gaggtccgaa cgtcgcagcg gcggtgttgg
     7561 cagcctccgc cgcctcggcg atcatctcgc cttgagggtc attgccggac tggcgttcgc
     7621 tgcggacgtt ggttgcggcc cgtggggaac taaccgggag aatcagctat gagctcaacc
     7681 aaccgaatga tacgtcacat cgttctggtc aagtttcgtg aagatgcacc ggacgagctg
     7741 cgccaggaac tcgtccggct ttcgcagtgg ggcagggatg cggactacgt gtccgggtac
     7801 gtctgcgggt ggggcatgca acccaatcta tatcccggag ctggtgagga atgggactgg
     7861 gggatgtccc tggacatggc ggaggaagat gccaaacgct atgcagagga ccctacccac
     7921 acggccattc cccaggaagt gatcgactgc gcggagaagt ttgcaatcct ggacttcctg
     7981 atggaatagg cgggccggct cggtcagggg tcttcgacga gcggggcatc gcttcccgac
     8041 tttgcggacg tggttgatga atgatgtgag aaaactcggc gcgctgctgt gtctactggc
     8101 cggttgcggc gcgtgcggcc ccacggggga tgctcccgga atcacggccg ctccagaccc
     8161 ggcagacgat gcgacgcaga cgcccgtcga tgggcattcg agtcgcgatt ccctggattg
     8221 gtccggcgtc tattcgggcg ttacgccctg cgccgactgt ccggggatcc ggaccacgct
     8281 cacgttgcat ggtgacggca gtttcgagcg aacccgcttc tatctagagc gaagcgccgc
     8341 tccgcagaga caggccgggc agttcctgtg gaacgcatcg ggaagcgcca taaccctgca
     8401 ggaaccggag ggccatggtc agcactacaa ggtcgcagag aacgcgttga ttcacctcga
     8461 ccgcgctggc aagccgatta ccggtgagct ggcgggacgc tacgtgctga gcaagcaccg
     8521 ccgtgacccg gccatcgaag gcaggaggtg gacgctcgtc gcgcttcagg gcaggccgct
     8581 cgaggcggat gcgcctggaa gcacgccctc cctgttgctc gatccggagc agtcgctcgc
     8641 tgccgaatct tgcgagcctg gccttcacac cgcgaatcat tccttgtagc aaagtggcac
     8701 cagatcccgc cgcgcccgta cccgaccttc atggggctcc agtgcgaaga gctggtagcg
     8761 tgggtcgaag tcgccggaag cgacctcgtc gacccgttcg aacaccgacc tgtatcggtc
     8821 gtaataatcc aggccgggcg cgcgcacatg atggtgttcg tccgcccggg cctctccgta
     8881 ctcgacggtc tgttgcgctt gtatcggcac aggcaggatc gccaggccac ccggacgcag
     8941 tactcgatgg atttccgcta tcgcgcggcg atcatcctgc acgtgctcga gcacgtggga
     9001 ggcgtacacc agatcgaggc tttcgtccgc gaatggcagt tgttcgagat tcgccaggca
     9061 atccacgccg ccggcaaatc ggtccgccga aatgtaccgt cggctcaagg tacggaaccg
     9121 ttgctccaga aacggttctg gagccaggtg caggatgcgg gcgttgcgga acagccgctg
     9181 atccaggccg tccagcacca gagcttgcag ccggtgccgc tccaggcagc ggcaggcagg
     9241 acagcgagcg tgcagtcgca cgccctgagg ctgcaccaag tctgcaaaag gcccgcggta
     9301 accgcatacc gggcagcagt aagcgtgggg cttgcgtccc tcggccgaca accggaaccg
     9361 cgtggtgcgg gccaggcgcc gaaatgcttc cgccaggttc ctcatttccg gtcggcgtcc
     9421 ttcagcaccg acagcagcac ggcttcgagc ttgccggtct gggaacgcgt gtcaaacttc
     9481 tcaaccatgc gtcggcgccc cgctgtcccg agttgtgcgc gcagcgccgg gtcgtttcct
     9541 agccgcgcca tggcccgcgc catgccggca acgtctccta cgtcgatcag aaatccggtt
     9601 tcgttcgcaa cgacctgatc cgggatgcca ccaacatccg tgctgacgac cggcaatccc
     9661 gctgaggccg cttcggccag ggcgacgcca aaaccctccc ggtgtgagtg ctggacgtag
     9721 atatcggagc cgctcagtag ttctgcaacg gctgcgggcg tcaacgctcc ggcgaaggtc
     9781 acccggctgg tgatgccgag cgagtcggtc agagccctta agcgttcccg atcgggacct
     9841 tcgcccacga tggtcagctc agcgtcgcca agggcttgcc ccagcttcgc gaaggctaac
     9901 agacactctt ccatgccctt ttcctctacc aggcgcccga cgctgacgaa tcgtattggg
     9961 cccggctcgg ttcgctggcc gggcacgaac atttgcgtgg gcacgccgca gggaatgacg
    10021 tgcacttggt ccgtggggag gcccagccct tcgagaatcc gaagctgaaa gctcccaacg
    10081 acaatcgagg cgtcgaagtg ccggaaatgg cgcagtaggc tggaccggta aaaccagcgg
    10141 tccagcattg cggccagatc cattccgtga aagtgggcta ccagggggat gcccagagag
    10201 cgtgcaaccg gtagtagtcg cagcgcggtg tggccgaaat gacatagcac cacgtccggg
    10261 tccagagcgc cgagctcctg gcttagggcc cgggcttccg cgcccagggc gccaaaaaaa
    10321 ttccggtccc ctgcatgcct caagcggaac atccaacggc ctgcgcccgc ataaggttct
    10381 gggtcaaagg gtagctctac caccggcgcc cctagcggcg gcgtccccgc caccgtctcc
    10441 caggtgagga ccgagggcgt gaacgcgttc attgtctgca gttgcctggc tacccaggtc
    10501 tggctgggca agcccagacg ttgcgcgacc accacgactt ttctccggcc ctgcactgtg
    10561 ctcggatttc cctcaggtac tcgttagttc tgccacggac cgacgatgat tgccaggcgc
    10621 cgcattgcgg cccgggtcca ggtcccacgc attcggcttc gtagctgcca aatcctgcgc
    10681 gacagtaccg cttttagatc tccggtgcgc gtcacgtcgg cgatgtaggt gggcgatccg
    10741 cccagacaat cgacgattcc atacccgcac gagtggagga accgcgccaa ctcggcgaac
    10801 tgcgcagggc cgtgggcttc cacacacaga agcggcatgt ccctggttat ggtttcctct
    10861 gcgccctgca gcacgtcgat ttccgcccct tccacatcga tcttgatcag caccacgggc
    10921 tcccagccag ccatctccgc cactgccacg tcgatggtgg taatcgccag ggtgccgtca
    10981 tcacgggggt ccaccgagac ggtgccgcga ttgcccgccg caccctgacg cagctcggca
    11041 cgcccgatgc ggttgccaag ggcgacgttt tgggccacga cggagcgcgc gtcgttgtct
    11101 ttcaggttct ggctcagcac ggtgaaagtt tccggatctg cctcgaaagc cagtaccgga
    11161 gccagcccgc agattcgaga gaagaagacc gagtgggtgc caatgaacgc gccggcatcc
    11221 accgcggcgc ccttcctgcc gttgagctga tgcaatatct tcagcagcac gctctgctcg
    11281 taaattgcgc cagactcgcg catcaccgcc agcacatgat catccgcgcc gacaccccga
    11341 aagttcagcg caacgccctc aacccggacg cttatggaag attcgctatc cattaccacc
    11401 gccgtctaga accgactgcc gcagggcggg caacagcgta ctcagctgct gcctggatcg
    11461 cttccacacg caggctgttg ccagcacgca gaggatgctg accagagcgg ccgcagcttc
    11521 cgccagcagc ccccgctcca gccagcgagc cagaaggtct tcggagagca gcgcgacgca
    11581 gagcatgagg acgctcagtg ttaccggcga agcggcggtg ttgataacat ccttcatcgc
    11641 caccggggtg tcgcgacatg cgatcgcagt gaggggcagc atcagcccaa ccaccacacc
    11701 gctgaaagtc caggccatgg ccccggtccc gaaaggtaat gccgctgcaa acgcgacgat
    11761 cagcaccacg gcattgacca tgctgacctt catgaagccg cccgccctcc cggtggcaat
    11821 gtacagccag ccaagtgcgt tgagcaccgg ttgcaccaag gctgccagag ccagtgcccg
    11881 aaacagcacg acggtctccc cccagcccgg accgaggacc agcaggatga tcgtgtccgc
    11941 gaaggcaacc agcagcgcaa cggcaggcat cgtcagccac aatattgcgc ccagaatgcc
    12001 cagaaaatag cgtcggaatc ggtccggctc gcgcgccagg cggctcagta ccggcaccgc
    12061 aatccgggtc agcgggtcgt tgatttgtcg caccggcatc agcagcaatc ggtaagcctg
    12121 gttgtagtag cccagcgccg ctgcacccca gtaccagccg atcagcaggt tgtccgcgtt
    12181 gcgggccgca tagccggcga ttccggagac ggtaaggcgg ccggcgaacc cgagctgtgc
    12241 ccgggtctcg aataggcgct tcggcaaacc tggccgccag tcgcagcaca aatagtagag
    12301 cagagtgctg acggcggcct gagctagcgg catcaggatc aaagcccagt aattgcgcgc
    12361 cgccagcgcc cagagcacgc ccgctgcggc tcccgccacc atggcggaga tttcgatccc
    12421 ggccagaagc cgataccgca tctgccggtt gagcattgct tgctgctggg tatgcaaacc
    12481 tcccatgaag aagaccagac ttatggcgca ggtggcgccg acgagcttgg cttccccgta
    12541 gaaccaggcc acgaggggcg caagcgccat ggttatcagc attagcgtca ggccaagccc
    12601 tgcattgatc cagaacaggt tggtcacctg ttgagggctg agcacgatgc tctgcatggt
    12661 cgccatgctc aggccgccgt ccttgagcag aaatagaaag cccgtgactg cggttatcat
    12721 tgcgatcagg ccgaaatccg cgggcgccag caaccgcgcc agtatggcga tggcgctgat
    12781 ctgaactgcg agcttgatcc cctgtgcggc cagggtaatc atgccgcccc gtgcagttcg
    12841 gccgcttagg ccggtgccct ggtcgacctc tgcagaccag ggttcattct caatggggga
    12901 gcgaggctcg gacatgaatc tgaaactggc gacaatacgt tgaattctgg cacatttttc
    12961 atgtaatgcc cgtgcgcccg atgagtcggc aacagcaaac gcaccgtaac agattgaaag
    13021 tagcgtttag ccctttctgg gggtcggatg gccatgccgg aatgccgagg attgagcggt
    13081 tcgtccgctg gttacagcat gcgggggcat cgatcgtggt cgtggccgcc ggttgcgagg
    13141 acgacctgag tcgagaggaa gccattgtcg tctaccgcag ccgcgatccg ctcggcttgt
    13201 accgcactga tccggatcgc cccgcacccg ccccccgccg gcccaaccgc tttcgcagaa
    13261 tgctttccta tctgctcttc gtgcccgatc cgagccgggt atgggccagt cggctggccg
    13321 cagaccggaa gctgctggag gtgcttcagg gcactaccca tgtgcttgca tcgagcccgc
    13381 ctgaatccgt tcaccctggc gcggcacggc ttgcccgccg tctcggcgct caatacataa
    13441 tggacatgcg tgacggctgg ctggacgaac ctctgcggcc acttctgcag cgttcggctc
    13501 tgcgccgctg gctggaacgg cgcctggaag cgcgctgtat tgctgccgct gctgcaattc
    13561 tggtgacatc ggacagttgg cgctcgctgc tcagcgcgag gtatcgccta gcggaaccgc
    13621 gcatccgcgt gctcaccaac gcttaccccc caacgcggca ttatccgcca gccgcgcctg
    13681 tctcggccgg gttgctggtt catgccgggc gatttgaggg ttccgatcca cggcggcgac
    13741 ctgccggtct cctggaaccg ctgctctcag cggtgtgcag gaaccagtcc ttgcaaggcg
    13801 agattctcct tcccggcgcg cacactccgg gagaccttct cgctctcgag agcttcgcga
    13861 ccgagttcgc ggcgattgga tggaagcttc gaatcagcac gccgatatcc cgaccccagc
    13921 tgttgtctga gttgaacatt gctggcgggt tgttgctgct atccgcatcg cgggcacaga
    13981 tacccggtaa actgttcgag tatttgccca cgggtcgacc gatcctggcg ctcgttcctg
    14041 gtgacggtgc cacgtggcgg ctgtgcgagg cactggaaca ggtttttctg gtcgacgccg
    14101 gcaaccccgg agcctttgcc ggaaggatcg atgagttcct tgctgccgcc cacgatgaca
    14161 agcgattccc cgtgccgccc gagtactcgg aggacgcctt agcggaggtc ttcctgtcgt
    14221 cgctcgtgac gccggaatgc gccatcactg cagatgattc tgcaggcgca ggtgcgaagg
    14281 ggcttaacgg gaaggctgat ctttagtctt ggaaccccac gaacgaaacg gcttcgtcca
    14341 cgggtttgcg ctctgaaacc acggcgttgg ccggaaagct gtcgtccgga aagcccatgg
    14401 ccacgcagat catgatcacc tcgtcgtccg gaatgcgcgc gtgctcgcga acgacggggg
    14461 actgcatgat gccctggctg ttgatgacgc atccaagccc cctggaccag gcggcgttga
    14521 cgagcgcgtt ggtgaccgct ccgcagtcga agggcgcgat atcgccgccg tggatggatc
    14581 ggtcgtaggt caccacgacc gagaccgggg cgtcgaactg acgaaatcca cgcagaaccc
    14641 agtcctgccg gcggggtttg tcgccccagt cgatgcccat ggcttcgaac agttgccccg
    14701 cgatctgttt ctgccgttcc cggtgctccc ccttgtagcc gccgtgcccg cggaactccc
    14761 tggaatccgg cacgccggca aggttccgct cggtattgcc ttcgcgaatc cggtccagcg
    14821 gctctccgct gaccacatga aagtgccagg gctgggtatt gagcgaggag ggcgctcgca
    14881 tcgcgatttc cacaacctcg cgtatcagcg ccttgggaac cggctccttg ctgaagcctc
    14941 ggatgctccg gcggccgaga acgacttcat cgtattccaa cgatggctcc tgagtgagaa
    15001 agctcggatt ctacctcgtt tgccgggttg accagctccc gtccaggctg ggcatacggg
    15061 catccagagc tgcgacgacg gcgcgtcaga gcccgatcat ctcccccacc cccgcataga
    15121 aagcccggtg ggcctcgtcg ctcagggttc ccagctgtcg ctcgcacacc gccatggctt
    15181 cgccggaacc ttccacatgg ggatagtcgc tggagaagca gagcatctcg ggaaccaggt
    15241 cgatggtggg gtgtagaggt tgcgaggcca ccagcggggt cacgcgaacc tgtcggcgca
    15301 ggtactcgga gggcgagagc ggcaggttgt acgcggatcc cgcaaagtcc ggccggtagg
    15361 cgccatcttc cagcgcccgg tgaccggccc gccccacgcc ctggtccagg gcgtcgagca
    15421 gaggcggcag ccaggcgatg ccgacttcct ccacgatcac agtaagcttg gggtgtcgct
    15481 cgaacacgcc atcgaaggcc agagcggcca gcagcagctg cggagcgagc tgggggttga
    15541 ccaccatgtt cagaaagctg taggtcatca gatcgtcggc accgttgttg gcccacccgg
    15601 ggttgatgcg ttcccgggaa aatccgacgt gggcgatggc cgccatgccc aggtcctcgg
    15661 ccgccgacca cagcggttcg aaatccgggt gggtcagcga tttggccagc gcgggctggc
    15721 cgttgcgtgc gaatcgcggt ttgcccaccg gggactccgg gatcgcgaac gcccggctgc
    15781 cgcgctcgcg catgcgcgtc atctcctcga tggaccgcgg gacgttggac aggtcgatct
    15841 gtgtgaccgg aatgagccgg tccgcgtggc cgtgcacctg atcggccgcc catcggttcc
    15901 agcactggcg aatttcgccc atgagctcga agcgtcgtgc gcgtgctgcc tggacgaacg
    15961 ctcccaccag gaacgtgggg ttcaggaact ggacgtcgat gcctcgttcg tcgcagaacg
    16021 ccaggcgcgc ctcggctccg cagaaagggt cgtcgtctga tccacactga cgctccggtt
    16081 ggcgctggtc ggtcatctcc aggtgatgca cgaagccagg caggatggtt tcgtagccac
    16141 ccgagggggg ctgcccctcg gacagctcgg cgagagcggg attcgcgatc ccgaaaaccg
    16201 aattagccac gtccatgaag cgtgccggcg gccccaactt cccggcgagc gcggggtctg
    16261 tctgctcgat ccagtcgagc ggctcgtgga gatgggcgtc gacgtcgatc acgcggatcg
    16321 cgtgaatccc cccagagtca cgagtcattg cggttgtgcc gtggcctgcc cgtcgagctc
    16381 cgcgaaggcg tgcttggcgg cccgcatgcc ctcgactgcc cttggaaacc cggtgtacag
    16441 gcacaggtgg gtcatgatct cctcgacctc agcgcgcgta aggccatggt tgagacccca
    16501 ggggatgtga aaggcgagct cggcctcctg gccgagggcg gtcaggatgg cgacgaccac
    16561 cagggagcgg tctctgcggg aga
//
LOCUS       DBNHLM010000384         5232 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04847
            SRR25158336_k127_298833, whole genome shotgun sequence.
ACCESSION   DBNHLM010000384 DBNHLM010000000
VERSION     DBNHLM010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414255
            Sequence Read Archive: SRR25158336
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:50:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,571
            CDSs (total)                      :: 3,538
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158336_k127_298833"
                     /isolate="MAG04847"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.19 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="AC16QF_17835"
     CDS             complement(<1..800)
                     /locus_tag="AC16QF_17835"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR6450472.1"
                     /translation="MSVPTSRTSAAALLACLLVAAAATEAASYRLPEGGEDVIGASIS
                     LTLAYEDTFAGVAQQFGVGYQELVDANPGVDPWLPGDGARIVMPTAYVLPSAPREGIV
                     INVAEYRLYYYPPDRGSVITFPVGVGRMDFPTPLISTKVVTRIENPSWTPTDAARREH
                     AEMGDPLPHVVPPGPDNPLGHLAIQLSAPGYFIHGTNKPFGVGQGVSHGCVRLYPGHI
                     ETLSELVPNGTPVYIVNEPYKVGWRDGELWIEAHRNLYGENQASELVAL"
     gene            948..1787
                     /locus_tag="AC16QF_17840"
     CDS             948..1787
                     /locus_tag="AC16QF_17840"
                     /inference="COORDINATES: protein motif:HMM:NF046782.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR6450473.1"
                     /translation="MPINPDAVGAEGRPVRRSWSSKDALLYAVGVGSGTDELAFTTET
                     TKDVAQRVLPTFAVIIGGGGVPMDAIGSFNPALMVHGEQGIELYDEIPPDGEIDSVGK
                     VTAIWDKGSAAVLEFESTSTNVATGKPLLKTRTSLFCRGEGGWGGDRGPSEKVEFPDR
                     EPDHQVTYATREDQALTYRLSGDRNPLHSDPSFAALGGFEKPILHGLCTWGFTGRALL
                     HTLCDGDPSRFKSMNSRFSKPVIPGDELTISIWVVDEGRALFRTTNQNGDAVIDQGVF
                     TYA"
     gene            1813..2205
                     /locus_tag="AC16QF_17845"
     CDS             1813..2205
                     /locus_tag="AC16QF_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6450474.1"
                     /translation="MIELVLRLSLWISLPLNAGAAYILAFPSSWPGQLLGLPAAPDPV
                     YAYLAAYLVALLGLAYGWMALQPSIVRPLLWLGALGKGGAFAVAAALWLAGLARAPLA
                     AAVTVDLLLAAFWIAWLTGTRGKASADG"
     gene            complement(2259..3653)
                     /locus_tag="AC16QF_17850"
     CDS             complement(2259..3653)
                     /locus_tag="AC16QF_17850"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6450475.1"
                     /translation="MTDLHYLSLAGVCRRMKSGELSASAVTEAILARIEALDSNLNSF
                     ATVMADEARAVAARLDQDRADGKPLGALHGVPVAVKDLFDTRGVVTASGTRVMGERVP
                     DEDATVVRRLKDAGAVIVGKTQLTEGAYGTHHPDVDPPKNPWDGDYWPGVSSSGSGVA
                     VAAGLSFGALGSDTGGSIRFPSACCGLVGIKPTYGRVSRYGAFPLAESLDHVGPMARS
                     VEDAARLLQVLAGADPYDVTTQPDPVPNYAASVADTLPGLRIGVDWDYVSKGVDEPVV
                     ETIREALEIFRELGATVVDIEVPASAATLVRGWGVTCGMECARAHRDFYPDRKAEYGP
                     VLAAFIDYGRAVSREDYGLLETVRAEFRDGLNGCLESADVIIAPCMPELPPPADALAD
                     GPRRPSDRADLITFTAPFNYSGHPTITLPAKLERGSLPRAFQLIARPLGEPTLVRVGS
                     AFESALGLDAHPIP"
     gene            complement(3650..5047)
                     /locus_tag="AC16QF_17855"
     CDS             complement(3650..5047)
                     /locus_tag="AC16QF_17855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6450476.1"
                     /translation="MTDVVVAELIEALGEDCVLTGEDVHSRQAGIWRSDTIQAKALVR
                     PRTTAEVSRTLAICNAHGQSIVPHGGLTGLVESAITGPDDVALSLERMNRIEEINPVD
                     RTMVVQSGVILQVLQEAAEEHQLMFPLDLGGRGSCTIGGNISTNAGGNRVIRYGMTRD
                     MVLGLEAVLADGTVLSSMNQMIKNNAGYDLKQLFIGTEGSLGVVTRAVLRLREQPACR
                     ATLLVSLDEFAKLAQFLKHMDAALGGALSAFEVMWNNFYTLVTTPPAENQAPIPRDYP
                     YYALVEAMGSDDAAVEAALAEAYEKSLITDAVIAQSEAQRLALWALRDSVEHTARYAP
                     IYTFDVSLRISHMESYLAEVNARLEARYQDVNNFTFGHMGDGNLHLVISVGEGGPETR
                     RAVEACVYEPLAAIQGSVSAEHGVGLEKKPYLGISRSEQEIELMRTIKRALDPKGILN
                     PGKIFDSASVNEAAA"
BASE COUNT          910 a         1702 c         1677 g          943 t
ORIGIN      
        1 agcgcaacga gttcggaagc ctggttttcg ccatataggt tgcggtgcgc ctctatccag
       61 agctcgccat cgcgccatcc caccttgtag ggctcattga cgatgtatac gggcgtcccg
      121 ttcggaacga gctccgacag cgtctctatg tggcccggat acaggcggac gcagccgtgg
      181 ctgacaccct gacctacgcc gaacggtttg ttggtgccgt gaatgaaata tccaggcgcg
      241 ctcagctgaa tggctagatg ccccagaggg ttgtcaggcc ctggcggcac cacgtgggga
      301 agcggatcac ccatctctgc atgctcccgc cgggccgcat ctgttggggt ccagctaggg
      361 ttctctatcc tggtgaccac cttggtgctg atgagcggcg tggggaagtc catccgccct
      421 acccccaccg ggaacgtgat gaccgagccg cgatcaggcg gatagtagta gagccgatac
      481 tcggcgacgt ttatgacgat cccttccctt ggcgcggagg gcagcacgta ggcggtgggc
      541 atgacgatac gggcaccatc tccgggtagc cagggatcca ccccggggtt ggcatccacc
      601 agttcctgat agcccacgcc gaattgctga gccacacctg caaaggtgtc ttcgtaggcg
      661 agcgtcaggc tgatgctggc gccgatgacg tcctctccgc cttcgggcag ccgataggac
      721 gccgcttcgg tcgcggccgc cgcaacaagc aggcaggcca gcagggctgc ggcagaagtc
      781 cgtgatgtcg ggacagacaa gtcgttctca aaccatcggc cgggaggacg ccattgtccc
      841 catctggatt cccaggtcca gtccgctgcc gcggctgcat tcgcgttgat cgcaatgggg
      901 cccacggcta tggtagcggg catccatgta agcgggagga tcttcgcatg ccaatcaacc
      961 cagatgccgt cggcgccgag ggacgtccgg tgcgccgcag ctggtcgtcc aaagatgctt
     1021 tgctctacgc tgtgggcgtc ggttcaggca ccgatgagct cgctttcacc accgagacca
     1081 caaaggacgt ggcgcaaagg gttctgccca ccttcgccgt gatcatcggc ggcggcggtg
     1141 tgccgatgga cgccatcggg tccttcaacc ccgcgctcat ggtccatggc gagcagggca
     1201 tcgagctcta cgacgagatc ccgccggacg gagagatcga tagcgtgggt aaagtgaccg
     1261 ccatctggga caaaggctcc gcggcggtgc tggagttcga atccacatcc acgaacgtcg
     1321 ccacgggcaa gccgcttctg aagacacgaa cgagcctctt ctgccgcggc gaagggggct
     1381 ggggcggcga tcgagggccc agcgagaaag tggaatttcc cgaccgcgag ccggaccatc
     1441 aggtcaccta cgcgaccagg gaggatcagg cgctcaccta ccggctctca ggcgatcgca
     1501 acccgctgca ttcggatcct tcctttgcgg cgctcggcgg cttcgagaag cccatactgc
     1561 acgggctgtg cacgtgggga ttcaccggtc gcgccctcct gcacaccctt tgcgacggag
     1621 acccgagccg attcaagagc atgaacagcc ggttttccaa gcccgtgatc cccggggacg
     1681 agttgacgat ctcgatatgg gtggtggacg aaggcagggc tttgttcagg acgaccaatc
     1741 agaacggcga cgcggtcatc gatcagggcg tgttcacata cgcctgatag cgtgcgcctg
     1801 cggcgagcgg caatgatcga gcttgttctg cggctgtcgc tctggataag cctgccgctc
     1861 aacgccggtg ctgcctacat cctggctttc ccctcgtcct ggccgggtca gctgctgggc
     1921 ctgccggcag cgcccgatcc cgtgtacgcc taccttgcag catatctggt ggccctgctc
     1981 gggctcgcct acggctggat ggccctgcaa ccgagcatcg tcaggcccct gctgtggttg
     2041 ggcgcccttg gcaagggggg cgccttcgcg gtggcggcag ccctttggct tgccggtctg
     2101 gctcgcgctc ccctggcggc cgccgtgacc gtcgacctgc tgctcgcggc gttctggatc
     2161 gcctggctca caggcacgcg gggaaaggcg agcgctgacg gttgaacgct tgatttccgg
     2221 gtcaggcgta gccagcgacg ccgggcgtcg gggcccggtc agggaatggg atgcgcgtcc
     2281 aggccgagag cgctttcgaa cgcgctgccg acacgaacga gggtaggctc tcccaggggt
     2341 cgcgcgatga gctgaaaggc tctcggcagg ctgccccgtt cgagcttcgc cggcagggtg
     2401 atggtcgggt gaccggaata attgaaaggc gcggtaaagg tgatgagatc cgcccgatct
     2461 gacggccgtc gtggtccgtc cgccaatgcg tcggctgggg gcggcaactc gggcatgcag
     2521 ggagcgatga tgacgtcggc gctttccagg cacccgttga gcccatcgcg aaactccgcg
     2581 cggacggttt cgagcaatcc gtaatcctcc cgggaaaccg cccgcccgta atcgatgaag
     2641 gccgccagca cgggtccgta ttccgccttg cggtcgggat agaaatcccg gtgggcacgc
     2701 gcgcactcca taccgcaggt gactccccac cctcgcacga gcgtcgcggc actcgccggc
     2761 acctcgatat cgaccaccgt ggcgccgagc tcccgaaaga tctccagggc ttcccggatc
     2821 gtctccacca cgggctcgtc gacgcctttg gacacgtagt cccagtcgac gccaattcga
     2881 aggcccggca gggtatcggc cacagaggca gcgtagttgg gcactgggtc gggctgcgtg
     2941 gtgacgtcgt acggatcggc gcccgcgagc acctgaagca gccgggcggc gtcctcaacg
     3001 ctgcgcgcca tggggcccac gtgatccagc gactcggcta ggggaaacgc accgtatcgg
     3061 cttacccttc cgtacgtagg cttgatgccg acgagcccgc agcaggcgct cggaaaacgg
     3121 atgctgccgc ccgtgtccga gccgagcgcg ccaaaggaca gccctgccgc caccgccacc
     3181 ccggatcccg aagacgatac gccgggccag tagtcgccgt cccaggggtt cttcggcggg
     3241 tccacgtccg ggtgatgggt cccgtaggca ccctccgtga gctgcgtctt gccgacgatc
     3301 accgcacctg catccttgag ccggcgcacc accgtggcat cctcgtccgg aacccgctcg
     3361 cccatgaccc gagtgccgct ggcggttacc acgccgcggg tatcgaacag gtccttcacc
     3421 gcgacgggaa cgccgtggag ggcgcccagc ggcttgccgt ccgcacgatc ctgatcgagg
     3481 cgggcagcaa ccgcgcgggc ctcgtcggcc atgaccgttg caaagctgtt caggttcgag
     3541 tcgagggctt cgatacgggc cagtatcgcc tcggtgaccg ccgacgcgct gagctcgccg
     3601 ctcttcatcc ggcgacacac accggccagc gacaggtagt gaaggtcggt catgcagccg
     3661 cttcgttcac cgatgcgctg tcgaagatct tgccgggatt gaggatgccc ttgggatcga
     3721 gggcccgctt gatcgtcctc atcagctcga tttcctgctc gctgcgggaa atgccgagat
     3781 agggcttctt ctcgagacca acgccatgct ccgcggagac cgagccctga atcgcagcaa
     3841 gcggctcgta gacgcacgcc tccactgccc tgcgagtctc gggacctccc tccccgacgg
     3901 agatgaccag gtgcaggttt ccgtctccca tgtgaccgaa ggtgaaattg ttgacgtcct
     3961 gataacgcgc ttccaggcgg gcattgacct ccgcgaggta tgactccatg tgggagatgc
     4021 gcaggctcac gtcgaaagtg tagatcgggg cgtaacgggc cgtgtgctcc acgctgtctc
     4081 gcaacgccca cagcgccagg cgctgagctt ccgactgcgc gatgaccgca tccgtaatga
     4141 gagatttttc gtaagcctcg gcgagggcgg cttccaccgc agcgtcatcg ctgcccatgg
     4201 cctcgaccag cgcgtagtag ggataatccc ggggaatcgg cgcctggttc tccgcgggcg
     4261 gcgtggtgac cagggtatag aagttgttcc acatgacctc gaaggcggaa agcgcgcctc
     4321 ccagcgctgc gtccatgtgc ttgaggaact gcgccagctt ggcgaactcg tccagcgaga
     4381 cgagcagggt cgcacggcag gcaggctgct cacgcagccg caacactgcc cgggtgacca
     4441 ccccgaggct tccttccgtg ccgatgaaaa gctgcttgag gtcgtagccg gcgttgttct
     4501 tgatcatctg gttcatgctg gagagcaccg tgccgtcagc gagcaccgct tccaggccca
     4561 gaaccatgtc ccgcgtcatg ccgtagcgaa tgacccgatt ccctccggcg ttggtagata
     4621 tgttgccgcc gatggtgcag ctgccgcgcc cgcccagatc gagcggaaac atgagctggt
     4681 gctcctcggc agcttcctga agcacctgca gaattacccc gctctgaacc accatggttc
     4741 gatctaccgg attgatttcc tcgatccggt tcatgcgttc cagcgagaga gcgacgtcgt
     4801 ccgggccggt aatggcgctt tccaccagcc cggtcagccc gccatgggga acgatgctct
     4861 ggccgtgggc attgcagatg gccagggtgc ggctgacttc cgcagtggtc ctgggccgga
     4921 cgagcgcttt ggcctggatc gtatccgagc gccagatgcc agcctggcgg ctgtgcacgt
     4981 cttccccggt caaaacgcag tcttccccca aagcttcgat gagttcggct acgacgacgt
     5041 cagtcatggc ttgatgtcct ggaatgcggc ggaagccgag cttacctcag aagcgaacgt
     5101 cctgcctcaa ggccacccat ccccgctggc cgggagggtg cagcattgtt tacctttggt
     5161 tctcgcccga ccctcaacga gcacgtcatg gccagaacct tcaacaaagc ctactacgac
     5221 cgctactacc gc
//
